doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
29298890	13	0	theme	APOBEC3	2417:2423	arg1	effects					2368:2374	the effects	2364:2374	the effects of the host intrinsic restriction factor APOBEC3	2364:2423	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	2	1	theme	viral	526:530	arg1	intermediates					544:556	viral replication intermediates	526:556	viral replication intermediates	526:556	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	9	2	theme	opposite	1583:1590	arg1	polarity					1604:1611	the opposite (NcytoCexo) polarity	1579:1611	the opposite (NcytoCexo) polarity	1579:1611	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	6	3	theme	A3	1185:1186	arg1	proteins					1188:1195	the mA3 and human A3 proteins	1167:1195	the mA3 and human A3 proteins	1167:1195	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	9	4	theme	NcytoCexo	1593:1601	arg1	polarity					1604:1611	the opposite (NcytoCexo) polarity	1579:1611	the opposite (NcytoCexo) polarity	1579:1611	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	13	5	theme	restriction	2398:2408	arg1	APOBEC3					2417:2423	the host intrinsic restriction factor APOBEC3	2379:2423	the host intrinsic restriction factor APOBEC3	2379:2423	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	11	6	theme	Gag	1935:1937	arg1	protein					1947:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	11	7	theme	host	1999:2002	arg1	defenses					2018:2025	host innate immune defenses	1999:2025	host innate immune defenses	1999:2025	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	11	8	theme	innate	2004:2009	arg1	defenses					2018:2025	host innate immune defenses	1999:2025	host innate immune defenses	1999:2025	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	3	9	theme	mouse	682:686	arg1	mA3					697:699	mA3	697:699	mA3	697:699	gPr80 also increases the resistance of MLVs to deamination and restriction by mouse APOBEC3 (mA3).
29298890	3	9	theme	mouse	682:686	arg1	APOBEC3					688:694	mouse APOBEC3	682:694	mouse APOBEC3 (mA3)	682:700	gPr80 also increases the resistance of MLVs to deamination and restriction by mouse APOBEC3 (mA3).
29298890	0	10	theme	I	102:102	arg1	Protein					122:128	a Type I Integral Membrane Protein	95:128	a Type I Integral Membrane Protein	95:128	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	11	11	theme	MLV-encoded	1910:1920	arg1	protein					1947:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	1	12	theme	murine	171:176	arg1	MLVs					196:199	MLVs	196:199	MLVs	196:199	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	12	theme	murine	171:176	arg1	viruses					187:193	murine leukemia viruses	171:193	murine leukemia viruses (MLVs)	171:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	14	13	theme	protein	2505:2511	arg1	role					2487:2490	the elusive role	2475:2490	the elusive role of the gPr80 protein	2475:2511	Overall this study provides further insight into the elusive role of the gPr80 protein.
29298890	6	14	theme	mA3	1171:1173	arg1	proteins					1188:1195	the mA3 and human A3 proteins	1167:1195	the mA3 and human A3 proteins	1167:1195	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	0	15	theme	Membrane	113:120	arg1	Protein					122:128	a Type I Integral Membrane Protein	95:128	a Type I Integral Membrane Protein	95:128	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	1	16	theme	viruses	187:193	arg1	gPr80					161:165	gPr80	161:165	gPr80	161:165	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	16	theme	viruses	187:193	arg1	protein					152:158	The glycosylated Gag protein	131:158	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs)	131:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	8	17	theme	NexoCcyto	1365:1373	arg1	orientation					1375:1385	the NexoCcyto orientation	1361:1385	the NexoCcyto orientation of a type I integral membrane protein	1361:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	7	18	theme	purified	1288:1295	arg1	viruses					1297:1303	purified viruses	1288:1303	purified viruses	1288:1303	We also show that full-length gPr80 is detected in purified viruses.
29298890	10	19	theme	new	1698:1700	arg1	insight					1702:1708	new insight	1698:1708	new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1698:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	9	20	theme	type	1634:1637	arg1	typical					1623:1629	typical	1623:1629	typical	1623:1629	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	9	20	theme	type	1634:1637	arg1	population					1495:1504	a large population	1487:1504	a large population	1487:1504	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	6	21	theme	capsid	1219:1224	arg1	stability					1226:1234	capsid stability	1219:1234	capsid stability	1219:1234	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	9	22	theme	integral	1642:1649	arg1	proteins					1660:1667	integral membrane proteins	1642:1667	type II integral membrane proteins	1634:1667	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	8	23	theme	type	1392:1395	arg1	protein					1417:1423	a type I integral membrane protein	1390:1423	a type I integral membrane protein	1390:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	10	24	theme	complex	1719:1725	arg1	nature					1727:1732	the complex nature	1715:1732	the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1715:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	8	25	theme	integral	1399:1406	arg1	protein					1417:1423	a type I integral membrane protein	1390:1423	a type I integral membrane protein	1390:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	1	26	gly	glycosylated	135:146	arg1	gPr80					161:165	gPr80	161:165	gPr80	161:165	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	26	gly	glycosylated	135:146	arg1	protein					152:158	The glycosylated Gag protein	131:158	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs)	131:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	27	theme	Gag	148:150	arg1	gPr80					161:165	gPr80	161:165	gPr80	161:165	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	27	theme	Gag	148:150	arg1	protein					152:158	The glycosylated Gag protein	131:158	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs)	131:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	8	28	theme	protein	1417:1423	arg1	orientation					1375:1385	the NexoCcyto orientation	1361:1385	the NexoCcyto orientation of a type I integral membrane protein	1361:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	12	29	theme	extended	2104:2111	arg1	sequence					2120:2127	an 88-amino-acid extended leader sequence	2087:2127	an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum	2087:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	1	30	theme	host-encoded	308:319	arg1	SERINC3					370:376	SERINC3	370:376	SERINC3	370:376	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	30	theme	host-encoded	308:319	arg1	SERINC5					383:389	SERINC5	383:389	SERINC5	383:389	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	30	theme	host-encoded	308:319	arg1	APOBEC3					361:367	APOBEC3	361:367	APOBEC3	361:367	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	30	theme	host-encoded	308:319	arg1	factors					344:350	host-encoded retroviral restriction factors	308:350	host-encoded retroviral restriction factors	308:350	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	5	31	theme	gPr80	901:905	arg1	function					889:896	the function	885:896	the function of gPr80	885:905	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	2	32	theme	immune	448:453	arg1	restriction					455:465	host innate immune restriction	436:465	host innate immune restriction	436:465	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	2	33	theme	cytosolic	581:589	arg1	sensors					595:601	cytosolic DNA sensors	581:601	cytosolic DNA sensors	581:601	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	1	34	theme	restriction	332:342	arg1	SERINC3					370:376	SERINC3	370:376	SERINC3	370:376	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	34	theme	restriction	332:342	arg1	SERINC5					383:389	SERINC5	383:389	SERINC5	383:389	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	34	theme	restriction	332:342	arg1	APOBEC3					361:367	APOBEC3	361:367	APOBEC3	361:367	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	34	theme	restriction	332:342	arg1	factors					344:350	host-encoded retroviral restriction factors	308:350	host-encoded retroviral restriction factors	308:350	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	7	35	theme	full-length	1255:1265	arg1	gPr80					1267:1271	full-length gPr80	1255:1271	full-length gPr80	1255:1271	We also show that full-length gPr80 is detected in purified viruses.
29298890	2	36	theme	host	436:439	arg1	restriction					455:465	host innate immune restriction	436:465	host innate immune restriction	436:465	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	12	37	theme	protein	2074:2080	arg1	gPr80					2028:2032	gPr80	2028:2032	gPr80	2028:2032	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	12	37	theme	protein	2074:2080	arg1	variant					2039:2045	a variant	2037:2045	a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum	2037:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	0	38	theme	Full-Length	0:10	arg1	Gag					25:27	Full-Length Glycosylated Gag	0:27	Full-Length Glycosylated Gag of Murine Leukemia Virus	0:52	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	1	39	theme	multiple	228:235	arg1	roles					237:241	multiple roles	228:241	multiple roles	228:241	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	12	40	theme	endoplasmic	2195:2205	arg1	reticulum					2207:2215	the endoplasmic reticulum	2191:2215	the endoplasmic reticulum	2191:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	9	41	theme	population	1495:1504	arg1	existence					1474:1482	the existence	1470:1482	the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins	1470:1667	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	10	42	theme	accessory	1751:1759	arg1	Viruses					1780:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses	1737:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1737:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	0	43	theme	Murine	32:37	arg1	Virus					48:52	Murine Leukemia Virus	32:52	Murine Leukemia Virus	32:52	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	1	44	theme	retrovirus	259:268	arg1	release					270:276	retrovirus release	259:276	retrovirus release	259:276	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	9	45	theme	Env-deficient	1509:1521	arg1	VLPs					1545:1548	VLPs	1545:1548	VLPs	1545:1548	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	9	45	theme	Env-deficient	1509:1521	arg1	particles					1534:1542	Env-deficient virus-like particles	1509:1542	Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity	1509:1611	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	5	46	theme	Gag	1080:1082	arg1	polyprotein					1095:1105	the Pr65 Gag structural polyprotein	1071:1105	the Pr65 Gag structural polyprotein	1071:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	10	47	theme	MLV	1741:1743	arg1	Viruses					1780:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses	1737:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1737:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	4	48	theme	N-linked	751:758	arg1	sites					774:778	three N-linked glycosylation sites	745:778	its three N-linked glycosylation sites	741:778	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	0	49	theme	Virus	48:52	arg1	Gag					25:27	Full-Length Glycosylated Gag	0:27	Full-Length Glycosylated Gag of Murine Leukemia Virus	0:52	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	9	50	theme	particles	1534:1542	arg1	typical					1623:1629	typical	1623:1629	typical	1623:1629	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	9	50	theme	particles	1534:1542	arg1	population					1495:1504	a large population	1487:1504	a large population	1487:1504	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	4	51	theme	accessory	717:725	arg1	protein					727:733	the gPr80 accessory protein	707:733	the gPr80 accessory protein	707:733	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	13	52	theme	glycans	2274:2280	arg1	contributions					2251:2263	the specific contributions	2238:2263	the specific contributions of gPr80 glycans and capsid stability	2238:2301	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	13	53	theme	specific	2242:2249	arg1	contributions					2251:2263	the specific contributions	2238:2263	the specific contributions of gPr80 glycans and capsid stability	2238:2301	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	10	54	dep	Viruses	1780:1786	arg1	persist					1847:1853	persist	1847:1853	persist in their hosts	1847:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	10	54	dep	Viruses	1780:1786	arg1	evolved					1793:1799	evolved	1793:1799	have evolved numerous strategies to infect	1788:1829	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	10	54	dep	Viruses	1780:1786	arg1	spread					1832:1837	spread	1832:1837	spread in	1832:1840	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	10	55	theme	Viruses	1780:1786	arg1	nature					1727:1732	the complex nature	1715:1732	the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1715:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	13	56	theme	factor	2410:2415	arg1	APOBEC3					2417:2423	the host intrinsic restriction factor APOBEC3	2379:2423	the host intrinsic restriction factor APOBEC3	2379:2423	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	2	57	theme	replication	532:542	arg1	intermediates					544:556	viral replication intermediates	526:556	viral replication intermediates	526:556	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	12	58	theme	Pr65	2065:2068	arg1	protein					2074:2080	the structural Pr65 Gag protein	2050:2080	the structural Pr65 Gag protein	2050:2080	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	4	59	theme	resistance	802:811	arg1	mechanisms					813:822	these resistance mechanisms	796:822	these resistance mechanisms	796:822	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	4	60	theme	glycosylation	760:772	arg1	sites					774:778	three N-linked glycosylation sites	745:778	its three N-linked glycosylation sites	741:778	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	6	61	theme	human	1179:1183	arg1	proteins					1188:1195	the mA3 and human A3 proteins	1167:1195	the mA3 and human A3 proteins	1167:1195	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	11	62	theme	glycosylated	1922:1933	arg1	protein					1947:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	11	63	theme	immune	2011:2016	arg1	defenses					2018:2025	host innate immune defenses	1999:2025	host innate immune defenses	1999:2025	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	6	64	attach	linked	1209:1214	arg1	stability					1226:1234	capsid stability	1219:1234	capsid stability	1219:1234	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	6	64	attach	linked	1209:1214	arg2	sensitivity					1137:1147	sensitivity	1137:1147	sensitivity to deamination by the mA3 and human A3 proteins	1137:1195	In fact, we demonstrate that sensitivity to deamination by the mA3 and human A3 proteins is directly linked to capsid stability.
29298890	0	65	theme	Integral	104:111	arg1	Protein					122:128	a Type I Integral Membrane Protein	95:128	a Type I Integral Membrane Protein	95:128	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	13	66	theme	capsid	2286:2291	arg1	stability					2293:2301	capsid stability	2286:2301	capsid stability	2286:2301	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	13	67	theme	host	2383:2386	arg1	APOBEC3					2417:2423	the host intrinsic restriction factor APOBEC3	2379:2423	the host intrinsic restriction factor APOBEC3	2379:2423	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	4	68	gly	glycosylation	760:772	arg2	three					745:749	three	745:749	three	745:749	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	4	68	gly	glycosylation	760:772	arg2	sites					774:778	three N-linked glycosylation sites	745:778	its three N-linked glycosylation sites	741:778	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	2	69	theme	capsid	484:489	arg1	stability					491:499	capsid stability	484:499	capsid stability	484:499	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	2	69	theme	capsid	484:489	arg1	feature					504:510	a feature	502:510	a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors	502:601	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	1	70	theme	leukemia	178:185	arg1	MLVs					196:199	MLVs	196:199	MLVs	196:199	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	70	theme	leukemia	178:185	arg1	viruses					187:193	murine leukemia viruses	171:193	murine leukemia viruses (MLVs)	171:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	5	71	theme	capsid	1016:1021	arg1	stability					1023:1031	capsid stability	1016:1031	capsid stability	1016:1031	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	14	72	theme	elusive	2479:2485	arg1	role					2487:2490	the elusive role	2475:2490	the elusive role of the gPr80 protein	2475:2511	Overall this study provides further insight into the elusive role of the gPr80 protein.
29298890	8	73	theme	I	1397:1397	arg1	protein					1417:1423	a type I integral membrane protein	1390:1423	a type I integral membrane protein	1390:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	9	74	theme	membrane	1651:1658	arg1	proteins					1660:1667	integral membrane proteins	1642:1667	type II integral membrane proteins	1634:1667	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	8	75	theme	membrane	1408:1415	arg1	protein					1417:1423	a type I integral membrane protein	1390:1423	a type I integral membrane protein	1390:1423	However, our results suggest that gPr80 is inserted in the NexoCcyto orientation of a type I integral membrane protein.
29298890	0	76	theme	Viral	77:81	arg1	Envelope					83:90	the Viral Envelope	73:90	the Viral Envelope	73:90	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	1	77	theme	glycosylated	135:146	arg1	gPr80					161:165	gPr80	161:165	gPr80	161:165	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	77	theme	glycosylated	135:146	arg1	protein					152:158	The glycosylated Gag protein	131:158	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs)	131:200	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	14	78	theme	further	2454:2460	arg1	insight					2462:2468	further insight	2454:2468	further insight into the elusive role of the gPr80 protein	2454:2511	Overall this study provides further insight into the elusive role of the gPr80 protein.
29298890	5	79	dep	characterized	871:883	arg1	revealed					931:938	revealed	931:938	revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein	931:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	4	80	with	protein	727:733	arg1	sites					774:778	three N-linked glycosylation sites	745:778	its three N-linked glycosylation sites	741:778	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	9	81	dep	type	1634:1637	arg1	proteins					1660:1667	integral membrane proteins	1642:1667	type II integral membrane proteins	1634:1667	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	5	82	theme	Pr65	1075:1078	arg1	polyprotein					1095:1105	the Pr65 Gag structural polyprotein	1071:1105	the Pr65 Gag structural polyprotein	1071:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	2	83	theme	DNA	591:593	arg1	sensors					595:601	cytosolic DNA sensors	581:601	cytosolic DNA sensors	581:601	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	12	84	theme	88-amino-acid	2090:2102	arg1	sequence					2120:2127	an 88-amino-acid extended leader sequence	2087:2127	an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum	2087:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	0	85	theme	Type	97:100	arg1	Protein					122:128	a Type I Integral Membrane Protein	95:128	a Type I Integral Membrane Protein	95:128	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	1	86	theme	retroviral	321:330	arg1	SERINC3					370:376	SERINC3	370:376	SERINC3	370:376	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	86	theme	retroviral	321:330	arg1	SERINC5					383:389	SERINC5	383:389	SERINC5	383:389	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	86	theme	retroviral	321:330	arg1	APOBEC3					361:367	APOBEC3	361:367	APOBEC3	361:367	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	1	86	theme	retroviral	321:330	arg1	factors					344:350	host-encoded retroviral restriction factors	308:350	host-encoded retroviral restriction factors	308:350	The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5.
29298890	4	87	link	N-linked	751:758	arg1	sites					774:778	three N-linked glycosylation sites	745:778	its three N-linked glycosylation sites	741:778	How the gPr80 accessory protein, with its three N-linked glycosylation sites, contributes to these resistance mechanisms is still not fully understood.
29298890	2	88	theme	innate	441:446	arg1	restriction					455:465	host innate immune restriction	436:465	host innate immune restriction	436:465	One way in which gPr80 helps MLVs to escape host innate immune restriction is by increasing capsid stability, a feature that protects viral replication intermediates from being detected by cytosolic DNA sensors.
29298890	11	89	gly	glycosylated	1922:1933	arg1	protein					1947:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	the MLV-encoded glycosylated Gag (gPr80) protein	1906:1953	Here we analyze the details of how the MLV-encoded glycosylated Gag (gPr80) protein protects the virus from being restricted by host innate immune defenses.
29298890	0	90	theme	Glycosylated	12:23	arg1	Gag					25:27	Full-Length Glycosylated Gag	0:27	Full-Length Glycosylated Gag of Murine Leukemia Virus	0:52	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	5	91	theme	parallel	1047:1054	arg1	involvement					1056:1066	their parallel involvement	1041:1066	their parallel involvement in the Pr65 Gag structural polyprotein	1041:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	13	92	theme	stability	2293:2301	arg1	contributions					2251:2263	the specific contributions	2238:2263	the specific contributions of gPr80 glycans and capsid stability	2238:2301	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	12	93	theme	Gag	2070:2072	arg1	protein					2074:2080	the structural Pr65 Gag protein	2050:2080	the structural Pr65 Gag protein	2050:2080	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	12	94	with	variant	2039:2045	arg1	sequence					2120:2127	an 88-amino-acid extended leader sequence	2087:2127	an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum	2087:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	9	95	theme	large	1489:1493	arg1	typical					1623:1629	typical	1623:1629	typical	1623:1629	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	9	95	theme	large	1489:1493	arg1	population					1495:1504	a large population	1487:1504	a large population	1487:1504	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	10	96	theme	protein.IMPORTANCE	1761:1778	arg1	Viruses					1780:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses	1737:1786	the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts	1737:1868	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	0	97	theme	Leukemia	39:46	arg1	Virus					48:52	Murine Leukemia Virus	32:52	Murine Leukemia Virus	32:52	Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.
29298890	5	98	from	involvement	1056:1066	arg1	polyprotein					1095:1105	the Pr65 Gag structural polyprotein	1071:1105	the Pr65 Gag structural polyprotein	1071:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	5	99	theme	structural	1084:1093	arg1	polyprotein					1095:1105	the Pr65 Gag structural polyprotein	1071:1105	the Pr65 Gag structural polyprotein	1071:1105	Here we further characterized the function of gPr80 and, more specifically, revealed that the asparagines targeted for glycosylation in gPr80 also contribute to capsid stability through their parallel involvement in the Pr65 Gag structural polyprotein.
29298890	13	100	theme	intrinsic	2388:2396	arg1	APOBEC3					2417:2423	the host intrinsic restriction factor APOBEC3	2379:2423	the host intrinsic restriction factor APOBEC3	2379:2423	This study dissects the specific contributions of gPr80 glycans and capsid stability in helping the virus to infect cells, spread, and counteract the effects of the host intrinsic restriction factor APOBEC3.
29298890	9	101	theme	virus-like	1523:1532	arg1	VLPs					1545:1548	VLPs	1545:1548	VLPs	1545:1548	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	9	101	theme	virus-like	1523:1532	arg1	particles					1534:1542	Env-deficient virus-like particles	1509:1542	Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity	1509:1611	Additionally, our experiments have revealed the existence of a large population of Env-deficient virus-like particles (VLPs) harboring gPr80 inserted in the opposite (NcytoCexo) polarity, which is typical of type II integral membrane proteins.
29298890	10	102	theme	numerous	1801:1808	arg1	strategies					1810:1819	numerous strategies	1801:1819	numerous strategies	1801:1819	Overall this study provides new insight into the complex nature of the MLV gPr80 accessory protein.IMPORTANCE Viruses have evolved numerous strategies to infect, spread in, and persist in their hosts.
29298890	12	103	theme	structural	2054:2063	arg1	protein					2074:2080	the structural Pr65 Gag protein	2050:2080	the structural Pr65 Gag protein	2050:2080	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	12	104	theme	leader	2113:2118	arg1	sequence					2120:2127	an 88-amino-acid extended leader sequence	2087:2127	an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum	2087:2215	gPr80 is a variant of the structural Pr65 Gag protein with an 88-amino-acid extended leader sequence that directs the protein for translation and glycosylation in the endoplasmic reticulum.
29298890	7	105	located	detected	1276:1283	arg1	viruses					1297:1303	purified viruses	1288:1303	purified viruses	1288:1303	We also show that full-length gPr80 is detected in purified viruses.
29298890	7	105	located	detected	1276:1283	arg2	gPr80					1267:1271	full-length gPr80	1255:1271	full-length gPr80	1255:1271	We also show that full-length gPr80 is detected in purified viruses.
29298890	3	106	theme	MLVs	643:646	arg1	resistance					629:638	the resistance	625:638	the resistance of MLVs to deamination and restriction	625:677	gPr80 also increases the resistance of MLVs to deamination and restriction by mouse APOBEC3 (mA3).
31054677	1	0	theme	drying	325:330	arg1	procedures					332:341	different drying procedures	315:341	different drying procedures	315:341	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	0	theme	drying	325:330	arg1	freeze-					373:379	freeze-	373:379	freeze-	373:379	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	0	theme	drying	325:330	arg1	vacuum-					364:370	vacuum-	364:370	vacuum-	364:370	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	0	theme	drying	325:330	arg1	air-					358:361	hot air-	354:361	hot air-	354:361	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	0	theme	drying	325:330	arg1	spray-drying					386:397	spray-drying	386:397	spray-drying	386:397	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	4	1	theme	retention	836:844	arg1	2.32 g/g					858:865	2.32 g/g	858:865	2.32 g/g	858:865	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	1	theme	retention	836:844	arg1	capacities					846:855	oil retention capacities	832:855	oil retention capacities (2.32 g/g)	832:866	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	6	2	theme	acid	1025:1028	arg1	content					1030:1036	a higher uronic acid content	1009:1036	a higher uronic acid content	1009:1036	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	4	3	theme	oil	832:834	arg1	2.32 g/g					858:865	2.32 g/g	858:865	2.32 g/g	858:865	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	3	theme	oil	832:834	arg1	capacities					846:855	oil retention capacities	832:855	oil retention capacities (2.32 g/g)	832:866	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	1	4	theme	residue	254:260	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	4	theme	residue	254:260	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	0	5	theme	shoot	96:100	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	5	theme	shoot	96:100	arg1	residues					102:109	shoot residues	96:109	shoot residues of bamboo (Chimonobambusa quadrangularis)	96:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	6	6	theme	uronic	1018:1023	arg1	content					1030:1036	a higher uronic acid content	1009:1036	a higher uronic acid content	1009:1036	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	0	7	theme	bamboo	114:119	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	5	8	theme	antioxidant	957:967	arg1	ability					969:975	the most potent antioxidant ability	941:975	the most potent antioxidant ability	941:975	Moreover, freeze-dried CPS exhibited the most potent antioxidant ability.
31054677	1	9	theme	BSR	263:265	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	9	theme	BSR	263:265	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	2	10	theme	antioxidant	513:523	arg1	activity					525:532	antioxidant activity	513:532	antioxidant activity	513:532	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	4	11	theme	air-	877:880	arg1	CPS					899:901	hot air- and vacuum-dried CPS	873:901	hot air- and vacuum-dried CPS	873:901	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	0	12	theme	Chimonobambusa	122:135	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	13	from	activity	63:70	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	13	from	activity	63:70	arg1	residues					102:109	shoot residues	96:109	shoot residues of bamboo (Chimonobambusa quadrangularis)	96:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	2	14	dep	compositions	462:473	arg1	the					449:451	the	449:451	the	449:451	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	15	theme	procedures	435:444	arg1	influences					404:413	The influences	400:413	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS	400:539	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	16	theme	chemical	453:460	arg1	compositions					462:473	chemical compositions	453:473	chemical compositions	453:473	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	4	17	theme	vacuum-dried	886:897	arg1	CPS					899:901	hot air- and vacuum-dried CPS	873:901	hot air- and vacuum-dried CPS	873:901	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	2	18	theme	drying	428:433	arg1	procedures					435:444	different drying procedures	418:444	different drying procedures	418:444	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	4	19	theme	better	804:809	arg1	5.17 g/g					818:825	5.17 g/g	818:825	5.17 g/g	818:825	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	19	theme	better	804:809	arg1	water					811:815	better water	804:815	better water (5.17 g/g)	804:826	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	3	20	contain	possessed	603:611	arg1	CPS					599:601	freeze-dried CPS	586:601	freeze-dried CPS	586:601	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	yield					640:644	the highest polysaccharide yield	613:644	the highest polysaccharide yield (8.86%)	613:652	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	%					683:683	9.42%	679:683	9.42%	679:683	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	content					670:676	uronic acid content	658:676	uronic acid content	658:676	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	weight					724:729	the lowest medium-high molecular weight	691:729	the lowest medium-high molecular weight (117.49 kDa)	691:742	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	content					756:762	glucose content	748:762	glucose content (5.81 mol%)	748:774	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	%					773:773	5.81 mol%	765:773	5.81 mol%	765:773	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	117.49 kDa					732:741	117.49 kDa	732:741	117.49 kDa	732:741	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	20	contain	possessed	603:611	arg2	%					651:651	8.86%	647:651	8.86%	647:651	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	1	21	theme	hot	354:356	arg1	air-					358:361	hot air-	354:361	hot air-	354:361	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	22	theme	present	190:196	arg1	study					198:202	the present study	186:202	the present study	186:202	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	2	23	theme	different	418:426	arg1	procedures					435:444	different drying procedures	418:444	different drying procedures	418:444	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	1	24	theme	-derived	267:274	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	24	theme	-derived	267:274	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	7	25	theme	CPS	1291:1293	arg1	production					1264:1273	industrial production	1253:1273	industrial production of high quality CPS	1253:1293	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	7	26	theme	industrial	1253:1262	arg1	production					1264:1273	industrial production	1253:1273	industrial production of high quality CPS	1253:1293	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	0	27	theme	properties	36:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.	0:181	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	7	28	theme	high	1278:1281	arg1	CPS					1291:1293	high quality CPS	1278:1293	high quality CPS	1278:1293	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	7	29	theme	quality	1283:1289	arg1	CPS					1291:1293	high quality CPS	1278:1293	high quality CPS	1278:1293	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	6	30	theme	glucose	1072:1078	arg1	content					1080:1086	lower glucose content	1066:1086	lower glucose content	1066:1086	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	6	31	theme	correlation	987:997	arg1	analysis					999:1006	correlation analysis	987:1006	correlation analysis	987:1006	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	0	32	theme	physicochemical	20:34	arg1	properties					36:45	physicochemical properties	20:45	physicochemical properties	20:45	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	3	33	theme	medium-high	702:712	arg1	weight					724:729	the lowest medium-high molecular weight	691:729	the lowest medium-high molecular weight (117.49 kDa)	691:742	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	33	theme	medium-high	702:712	arg1	117.49 kDa					732:741	117.49 kDa	732:741	117.49 kDa	732:741	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	0	34	theme	quadrangularis	137:150	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	34	theme	quadrangularis	137:150	arg1	residues					102:109	shoot residues	96:109	shoot residues of bamboo (Chimonobambusa quadrangularis)	96:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	35	from	residues	102:109	arg1	polysaccharides					75:89	polysaccharides	75:89	polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis)	75:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	35	from	residues	102:109	arg1	properties					36:45	physicochemical properties	20:45	physicochemical properties	20:45	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	35	from	residues	102:109	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	6	36	theme	lower	1066:1070	arg1	content					1080:1086	lower glucose content	1066:1086	lower glucose content	1066:1086	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	0	37	theme	antioxidant	51:61	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	3	38	theme	molecular	714:722	arg1	weight					724:729	the lowest medium-high molecular weight	691:729	the lowest medium-high molecular weight (117.49 kDa)	691:742	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	38	theme	molecular	714:722	arg1	117.49 kDa					732:741	117.49 kDa	732:741	117.49 kDa	732:741	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	2	39	theme	CPS	537:539	arg1	compositions					462:473	chemical compositions	453:473	chemical compositions	453:473	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	39	theme	CPS	537:539	arg1	characteristics					492:506	physicochemical characteristics	476:506	physicochemical characteristics	476:506	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	39	theme	CPS	537:539	arg1	activity					525:532	antioxidant activity	513:532	antioxidant activity	513:532	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	6	40	theme	CPS	1173:1175	arg1	activity					1144:1151	the better antioxidant activity	1121:1151	the better antioxidant activity of the freeze-dried CPS	1121:1175	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	1	41	theme	bamboo	209:214	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	41	theme	bamboo	209:214	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	6	42	theme	higher	1011:1016	arg1	content					1030:1036	a higher uronic acid content	1009:1036	a higher uronic acid content	1009:1036	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	5	43	theme	potent	950:955	arg1	ability					969:975	the most potent antioxidant ability	941:975	the most potent antioxidant ability	941:975	Moreover, freeze-dried CPS exhibited the most potent antioxidant ability.
31054677	1	44	theme	shoot	216:220	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	44	theme	shoot	216:220	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	3	45	theme	glucose	748:754	arg1	content					756:762	glucose content	748:762	glucose content (5.81 mol%)	748:774	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	45	theme	glucose	748:754	arg1	%					773:773	5.81 mol%	765:773	5.81 mol%	765:773	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	0	46	theme	drying	164:169	arg1	procedures					171:180	drying procedures	164:180	drying procedures	164:180	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	2	47	theme	physicochemical	476:490	arg1	characteristics					492:506	physicochemical characteristics	476:506	physicochemical characteristics	476:506	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	48	from	influences	404:413	arg1	compositions					462:473	chemical compositions	453:473	chemical compositions	453:473	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	48	from	influences	404:413	arg1	characteristics					492:506	physicochemical characteristics	476:506	physicochemical characteristics	476:506	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	2	48	from	influences	404:413	arg1	activity					525:532	antioxidant activity	513:532	antioxidant activity	513:532	The influences of different drying procedures on the chemical compositions, physicochemical characteristics, and antioxidant activity of CPS were investigated.
31054677	7	49	theme	above	1189:1193	arg1	results					1195:1201	the above results	1185:1201	the above results	1185:1201	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	0	50	theme	activity	63:70	arg1	Characterization					0:15	Characterization	0:15	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.	0:181	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	4	51	theme	freeze-dried	783:794	arg1	CPS					796:798	freeze-dried CPS	783:798	freeze-dried CPS	783:798	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	1	52	theme	different	315:323	arg1	procedures					332:341	different drying procedures	315:341	different drying procedures	315:341	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	52	theme	different	315:323	arg1	freeze-					373:379	freeze-	373:379	freeze-	373:379	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	52	theme	different	315:323	arg1	vacuum-					364:370	vacuum-	364:370	vacuum-	364:370	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	52	theme	different	315:323	arg1	air-					358:361	hot air-	354:361	hot air-	354:361	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	52	theme	different	315:323	arg1	spray-drying					386:397	spray-drying	386:397	spray-drying	386:397	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	3	53	theme	acid	665:668	arg1	content					670:676	uronic acid content	658:676	uronic acid content	658:676	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	54	theme	freeze-dried	586:597	arg1	CPS					599:601	freeze-dried CPS	586:601	freeze-dried CPS	586:601	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	4	55	contain	had	800:802	arg2	2.32 g/g					858:865	2.32 g/g	858:865	2.32 g/g	858:865	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	55	contain	had	800:802	arg2	5.17 g/g					818:825	5.17 g/g	818:825	5.17 g/g	818:825	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	55	contain	had	800:802	arg2	capacities					846:855	oil retention capacities	832:855	oil retention capacities (2.32 g/g)	832:866	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	55	contain	had	800:802	arg1	CPS					796:798	freeze-dried CPS	783:798	freeze-dried CPS	783:798	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	4	55	contain	had	800:802	arg2	water					811:815	better water	804:815	better water (5.17 g/g)	804:826	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31054677	3	56	theme	highest	617:623	arg1	%					683:683	9.42%	679:683	9.42%	679:683	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	56	theme	highest	617:623	arg1	%					651:651	8.86%	647:651	8.86%	647:651	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	56	theme	highest	617:623	arg1	yield					640:644	the highest polysaccharide yield	613:644	the highest polysaccharide yield (8.86%)	613:652	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	57	theme	lowest	695:700	arg1	weight					724:729	the lowest medium-high molecular weight	691:729	the lowest medium-high molecular weight (117.49 kDa)	691:742	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	57	theme	lowest	695:700	arg1	117.49 kDa					732:741	117.49 kDa	732:741	117.49 kDa	732:741	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	5	58	theme	freeze-dried	914:925	arg1	CPS					927:929	freeze-dried CPS	914:929	freeze-dried CPS	914:929	Moreover, freeze-dried CPS exhibited the most potent antioxidant ability.
31054677	3	59	theme	polysaccharide	625:638	arg1	%					683:683	9.42%	679:683	9.42%	679:683	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	59	theme	polysaccharide	625:638	arg1	%					651:651	8.86%	647:651	8.86%	647:651	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	59	theme	polysaccharide	625:638	arg1	yield					640:644	the highest polysaccharide yield	613:644	the highest polysaccharide yield (8.86%)	613:652	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	6	60	theme	freeze-dried	1160:1171	arg1	CPS					1173:1175	the freeze-dried CPS	1156:1175	the freeze-dried CPS	1156:1175	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	6	61	theme	molecular	1045:1053	arg1	weight					1055:1060	lower molecular weight	1039:1060	lower molecular weight	1039:1060	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	3	62	theme	5.81 mol	765:772	arg1	content					756:762	glucose content	748:762	glucose content (5.81 mol%)	748:774	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	3	62	theme	5.81 mol	765:772	arg1	%					773:773	5.81 mol%	765:773	5.81 mol%	765:773	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	0	63	theme	procedures	171:180	arg1	Effect					154:159	Effect	154:159	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.	0:181	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	6	64	theme	antioxidant	1132:1142	arg1	activity					1144:1151	the better antioxidant activity	1121:1151	the better antioxidant activity of the freeze-dried CPS	1121:1175	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	6	65	theme	lower	1039:1043	arg1	weight					1055:1060	lower molecular weight	1039:1060	lower molecular weight	1039:1060	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	1	66	theme	Chimonobambusa	223:236	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	66	theme	Chimonobambusa	223:236	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	3	67	theme	uronic	658:663	arg1	content					670:676	uronic acid content	658:676	uronic acid content	658:676	The results indicate that freeze-dried CPS possessed the highest polysaccharide yield (8.86%) and uronic acid content (9.42%), and the lowest medium-high molecular weight (117.49 kDa) and glucose content (5.81 mol%).
31054677	6	68	theme	better	1125:1130	arg1	activity					1144:1151	the better antioxidant activity	1121:1151	the better antioxidant activity of the freeze-dried CPS	1121:1175	Based on correlation analysis, a higher uronic acid content, lower molecular weight and lower glucose content might be closely associated with the better antioxidant activity of the freeze-dried CPS.
31054677	7	69	theme	optimal	1234:1240	arg1	method					1242:1247	the optimal method	1230:1247	the optimal method for industrial production of high quality CPS	1230:1293	Hence, the above results revealed freeze-drying was the optimal method for industrial production of high quality CPS.
31054677	1	70	theme	quadrangularis	238:251	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	70	theme	quadrangularis	238:251	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	0	71	theme	polysaccharides	75:89	arg1	properties					36:45	physicochemical properties	20:45	physicochemical properties	20:45	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	71	theme	polysaccharides	75:89	arg1	activity					63:70	antioxidant activity	51:70	antioxidant activity	51:70	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	72	from	properties	36:45	arg1	quadrangularis					137:150	bamboo (Chimonobambusa quadrangularis)	114:151	bamboo (Chimonobambusa quadrangularis)	114:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	72	from	properties	36:45	arg1	residues					102:109	shoot residues	96:109	shoot residues of bamboo (Chimonobambusa quadrangularis)	96:151	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	0	73	dep	Characterization	0:15	arg1	Effect					154:159	Effect	154:159	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.	0:181	Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures.
31054677	1	74	link	-derived	267:274	arg1	CPS					293:295	CPS	293:295	CPS	293:295	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	1	74	link	-derived	267:274	arg1	polysaccharides					276:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides	205:290	the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS)	205:296	In the present study, the bamboo shoot (Chimonobambusa quadrangularis) residue (BSR)-derived polysaccharides (CPS) were dried using different drying procedures, including hot air-, vacuum-, freeze-, and spray-drying.
31054677	4	75	theme	hot	873:875	arg1	CPS					899:901	hot air- and vacuum-dried CPS	873:901	hot air- and vacuum-dried CPS	873:901	Also, freeze-dried CPS had better water (5.17 g/g) and oil retention capacities (2.32 g/g) than hot air- and vacuum-dried CPS.
31826432	0	0	from	characterization	13:28	arg1	Sagittaria					79:88	Sagittaria	79:88	Sagittaria	79:88	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	1	theme	polysaccharides	212:226	arg1	fractions					199:207	different fractions	189:207	different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs)	189:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	4	2	theme	macrophage	1025:1034	arg1	proliferation					1036:1048	macrophage proliferation	1025:1048	macrophage proliferation	1025:1048	SPCs could significantly promote macrophage proliferation, NO release and phagocytosis.
31826432	0	3	from	fractions	199:207	arg1	L					257:257	Sagittaria sagittifolia L	233:257	Sagittaria sagittifolia L	233:257	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	2	4	theme	Molecular	594:602	arg1	weight					604:609	Molecular weight	594:609	Molecular weight	594:609	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	4	5	theme	NO	1051:1052	arg1	release					1054:1060	NO release	1051:1060	NO release	1051:1060	SPCs could significantly promote macrophage proliferation, NO release and phagocytosis.
31826432	3	6	theme	other	968:972	arg1	components					980:989	the other three components	964:989	the other three components	964:989	In addition, SPC-70 exhibited stronger antioxidant activity in vitro than the other three components.
31826432	0	7	from	Preparation	0:10	arg1	Sagittaria					79:88	Sagittaria	79:88	Sagittaria	79:88	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	8	theme	Sagittaria	233:242	arg1	L					257:257	Sagittaria sagittifolia L	233:257	Sagittaria sagittifolia L	233:257	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	1	9	theme	neutral	448:454	arg1	sugar					456:460	neutral sugar	448:460	neutral sugar	448:460	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	2	10	with	mannose	818:824	arg1	ratios					882:887	dramatically different mole ratios	854:887	dramatically different mole ratios	854:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	0	11	theme	ultrasonic	270:279	arg1	extraction					290:299	ultrasonic assisted extraction	270:299	ultrasonic assisted extraction (UAE)	270:305	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	11	theme	ultrasonic	270:279	arg1	UAE					302:304	UAE	302:304	UAE	302:304	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	12	theme	preliminary	123:133	arg1	characteristics					146:160	the preliminary structural characteristics	119:160	the preliminary structural characteristics	119:160	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	2	13	with	glucose	827:833	arg1	ratios					882:887	dramatically different mole ratios	854:887	dramatically different mole ratios	854:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	0	14	theme	sagittifolia	244:255	arg1	L					257:257	Sagittaria sagittifolia L	233:257	Sagittaria sagittifolia L	233:257	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	5	15	theme	S.	1141:1142	arg1	polysaccharides					1160:1174	S. sagittifolia L. polysaccharides	1141:1174	S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture	1141:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	0	16	from	bioactivity	34:44	arg1	Sagittaria					79:88	Sagittaria	79:88	Sagittaria	79:88	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	2	17	theme	monosaccharide	615:628	arg1	composition					630:640	monosaccharide composition	615:640	monosaccharide composition	615:640	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	0	18	theme	assisted	281:288	arg1	extraction					290:299	ultrasonic assisted extraction	270:299	ultrasonic assisted extraction (UAE)	270:305	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	18	theme	assisted	281:288	arg1	UAE					302:304	UAE	302:304	UAE	302:304	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	2	19	with	galactose	839:847	arg1	ratios					882:887	dramatically different mole ratios	854:887	dramatically different mole ratios	854:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	5	20	theme	L.	1157:1158	arg1	polysaccharides					1160:1174	S. sagittifolia L. polysaccharides	1141:1174	S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture	1141:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	2	21	theme	neutral	754:760	arg1	SPC-60					666:671	SPC-60	666:671	SPC-60	666:671	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	2	21	theme	neutral	754:760	arg1	polysaccharide					762:775	a neutral polysaccharide	752:775	a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios	752:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	2	21	theme	neutral	754:760	arg1	SPC-90					728:733	SPC-90	728:733	SPC-90	728:733	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	2	21	theme	neutral	754:760	arg1	SPC-80					705:710	SPC-80	705:710	SPC-80	705:710	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	2	21	theme	neutral	754:760	arg1	SPC-70					685:690	SPC-70	685:690	SPC-70	685:690	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	5	22	theme	polysaccharides	1160:1174	arg1	applications					1125:1136	applications	1125:1136	applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture	1125:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	0	23	dep	Sagittaria	79:88	arg1	L.					103:104	Sagittaria sagittifolia L.	79:104	Sagittaria sagittifolia L.	79:104	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	1	24	theme	ultraviolet	560:570	arg1	spectra					585:591	the ultraviolet and infrared spectra	556:591	the ultraviolet and infrared spectra	556:591	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	2	25	theme	mole	877:880	arg1	ratios					882:887	dramatically different mole ratios	854:887	dramatically different mole ratios	854:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	0	26	theme	structural	135:144	arg1	characteristics					146:160	the preliminary structural characteristics	119:160	the preliminary structural characteristics	119:160	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	27	dep	polysaccharides	317:331	arg1	SPC-80					350:355	SPC-80	350:355	SPC-80	350:355	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	27	dep	polysaccharides	317:331	arg1	polysaccharides					317:331	four new polysaccharides	308:331	four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90)	308:367	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	27	dep	polysaccharides	317:331	arg1	SPC-90					361:366	SPC-90	361:366	SPC-90	361:366	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	28	theme	biological	166:175	arg1	activity					177:184	biological activity	166:184	biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs)	166:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	1	29	theme	infrared	576:583	arg1	spectra					585:591	the ultraviolet and infrared spectra	556:591	the ultraviolet and infrared spectra	556:591	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	1	30	contain	contained	468:476	arg2	proteins					478:485	proteins	478:485	proteins	478:485	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	1	30	contain	contained	468:476	arg1	four					463:466	four	463:466	four	463:466	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	1	30	contain	contained	468:476	arg2	four					463:466	four	463:466	four	463:466	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	1	30	contain	contained	468:476	arg1	structure					513:521	their structure	507:521	their structure	507:521	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	1	30	contain	contained	468:476	arg2	acids					498:502	uronic acids	491:502	uronic acids	491:502	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	2	31	theme	different	867:875	arg1	ratios					882:887	dramatically different mole ratios	854:887	dramatically different mole ratios	854:887	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
31826432	0	32	from	Sagittaria	79:88	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	32	from	Sagittaria	79:88	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	32	from	Sagittaria	79:88	arg1	bioactivity					34:44	bioactivity	34:44	bioactivity	34:44	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	32	from	Sagittaria	79:88	arg1	fractions					64:72	polysaccharide fractions	49:72	polysaccharide fractions from Sagittaria sagittifolia L.	49:104	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	3	33	theme	antioxidant	929:939	arg1	activity					941:948	stronger antioxidant activity	920:948	stronger antioxidant activity	920:948	In addition, SPC-70 exhibited stronger antioxidant activity in vitro than the other three components.
31826432	0	34	theme	fractions	64:72	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	34	theme	fractions	64:72	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	34	theme	fractions	64:72	arg1	bioactivity					34:44	bioactivity	34:44	bioactivity	34:44	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	1	35	theme	uronic	491:496	arg1	acids					498:502	uronic acids	491:502	uronic acids	491:502	The results implied that except for neutral sugar, four contained proteins and uronic acids in their structure, which were further confirmed by the ultraviolet and infrared spectra.
31826432	0	36	theme	polysaccharide	49:62	arg1	fractions					64:72	polysaccharide fractions	49:72	polysaccharide fractions from Sagittaria sagittifolia L.	49:104	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	37	from	L	257:257	arg1	SPCs					261:264	SPCs	261:264	SPCs	261:264	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	37	from	L	257:257	arg1	polysaccharides					212:226	polysaccharides	212:226	polysaccharides from Sagittaria sagittifolia L. (SPCs)	212:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	37	from	L	257:257	arg1	fractions					199:207	different fractions	189:207	different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs)	189:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	38	theme	new	313:315	arg1	SPC-80					350:355	SPC-80	350:355	SPC-80	350:355	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	38	theme	new	313:315	arg1	SPC-90					361:366	SPC-90	361:366	SPC-90	361:366	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	38	theme	new	313:315	arg1	polysaccharides					317:331	four new polysaccharides	308:331	four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90)	308:367	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	5	39	theme	sagittifolia	1144:1155	arg1	polysaccharides					1160:1174	S. sagittifolia L. polysaccharides	1141:1174	S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture	1141:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	0	40	theme	fractions	199:207	arg1	characteristics					146:160	the preliminary structural characteristics	119:160	the preliminary structural characteristics	119:160	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	0	40	theme	fractions	199:207	arg1	activity					177:184	biological activity	166:184	biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs)	166:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	3	41	theme	stronger	920:927	arg1	activity					941:948	stronger antioxidant activity	920:948	stronger antioxidant activity	920:948	In addition, SPC-70 exhibited stronger antioxidant activity in vitro than the other three components.
31826432	5	42	theme	industry	1215:1222	arg1	development					1200:1210	the development	1196:1210	the development of industry and agriculture	1196:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	0	43	theme	different	189:197	arg1	fractions					199:207	different fractions	189:207	different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs)	189:265	Preparation, characterization and bioactivity of polysaccharide fractions from Sagittaria sagittifolia L. To ascertain the preliminary structural characteristics and biological activity of different fractions of polysaccharides from Sagittaria sagittifolia L. (SPCs) by ultrasonic assisted extraction (UAE), four new polysaccharides (SPC-60, SPC-70, SPC-80 and SPC-90) were successively fractionated by ethanol.
31826432	5	44	theme	agriculture	1228:1238	arg1	development					1200:1210	the development	1196:1210	the development of industry and agriculture	1196:1238	Thus, these results provided a reference for applications of S. sagittifolia L. polysaccharides which would benefit the development of industry and agriculture.
31826432	2	45	dep	weight	604:609	arg1	analysis					642:649	analysis	642:649	analysis	642:649	Molecular weight and monosaccharide composition analysis exhibited that SPC-60 (52.0 kDa), SPC-70 (294.9 kDa), SPC-80 (230.6 kDa) and SPC-90 (229.4 kDa) were a neutral polysaccharide composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with dramatically different mole ratios.
30456405	0	0	theme	physicochemical	66:80	arg1	characteristics					94:108	the physicochemical and sensory characteristics	62:108	the physicochemical and sensory characteristics of gluten-free	62:123	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	2	1	theme	bean	446:449	arg1	flour					451:455	fava bean flour	441:455	fava bean flour	441:455	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	5	2	theme	color	1019:1023	arg1	parameters					1025:1034	color parameters	1019:1034	color parameters	1019:1034	Xanthan gum increased the cakes' firmness after cooking; in what concerns color parameters, hydrocolloids did not interfere.
30456405	0	3	dep	cakes	125:129	arg1	based					131:135	based	131:135	cakes based on fava beans (Phaseolus lunatus)	125:169	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	1	4	theme	nutritional	236:246	arg1	value					248:252	low nutritional value	232:252	low nutritional value	232:252	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	2	5	theme	fava	441:444	arg1	flour					451:455	fava bean flour	441:455	fava bean flour	441:455	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	6	theme	nutritional	1346:1356	arg1	value					1358:1362	high nutritional value	1341:1362	high nutritional value	1341:1362	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	1	7	theme	value	248:252	arg1	sources					221:227	starch sources	214:227	starch sources of low nutritional value	214:252	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	0	8	theme	sensory	86:92	arg1	characteristics					94:108	the physicochemical and sensory characteristics	62:108	the physicochemical and sensory characteristics of gluten-free	62:123	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	9	from	Influence	0:8	arg1	characteristics					94:108	the physicochemical and sensory characteristics	62:108	the physicochemical and sensory characteristics of gluten-free	62:123	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	6	10	theme	higher	1186:1191	arg1	levels					1193:1198	higher levels	1186:1198	higher levels of moisture, proteins, lipids and ashes	1186:1238	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	2	11	theme	work	277:280	arg1	objective					259:267	The objective	255:267	The objective of this work	255:280	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	12	theme	high	1341:1344	arg1	value					1358:1362	high nutritional value	1341:1362	high nutritional value	1341:1362	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	2	13	theme	xanthan	478:484	arg1	gum					486:488	xanthan gum	478:488	xanthan gum	478:488	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	3	14	theme	bubbles	653:659	arg1	microscopy					631:640	microscopy	631:640	microscopy	631:640	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	14	theme	bubbles	653:659	arg1	viscosity					599:607	viscosity	599:607	viscosity	599:607	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	14	theme	bubbles	653:659	arg1	gravity					619:625	specific gravity	610:625	specific gravity	610:625	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	2	15	theme	%	523:523	arg1	proportions					525:535	0.5 and 1.0% proportions	512:535	0.5 and 1.0% proportions	512:535	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	16	theme	sensory	1107:1113	arg1	acceptance					1115:1124	better sensory acceptance	1100:1124	better sensory acceptance	1100:1124	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	6	17	theme	fava	1085:1088	arg1	beans					1090:1094	fava beans	1085:1094	fava beans	1085:1094	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	4	18	theme	specific	884:891	arg1	gravity					893:899	specific gravity	884:899	specific gravity	884:899	Hydrocolloids increased the viscosity, specific gravity, and incorporation of air into the batters.
30456405	6	19	theme	hydrocolloids	1158:1170	arg1	presence					1146:1153	the presence	1142:1153	the presence of hydrocolloids	1142:1170	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	3	20	theme	sensorial	823:831	arg1	properties					833:842	physicochemical (chemical composition, specific volume, texture and color) and sensorial properties	744:842	physicochemical (chemical composition, specific volume, texture and color) and sensorial properties	744:842	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	2	21	theme	cakes	365:369	arg1	production					332:341	the production	328:341	the production of gluten-free sponge cakes	328:369	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	2	22	theme	commercial	554:563	arg1	reference					565:573	a commercial reference	552:573	a commercial reference	552:573	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	23	theme	food	1310:1313	arg1	option					1315:1320	an excellent food option	1297:1320	an excellent food option without gluten and high nutritional value	1297:1362	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	2	24	theme	sponge	358:363	arg1	cakes					365:369	gluten-free sponge cakes	346:369	gluten-free sponge cakes	346:369	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	3	25	theme	air	649:651	arg1	bubbles					653:659	the air bubbles	645:659	the air bubbles	645:659	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	2	26	theme	fava	293:296	arg1	beans					298:302	fava beans	293:302	fava beans (Phaseolus lunatus)	293:322	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	2	26	theme	fava	293:296	arg1	lunatus					315:321	Phaseolus lunatus	305:321	Phaseolus lunatus	305:321	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	27	theme	proteins	1213:1220	arg1	levels					1193:1198	higher levels	1186:1198	higher levels of moisture, proteins, lipids and ashes	1186:1238	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	2	28	theme	gluten-free	346:356	arg1	cakes					365:369	gluten-free sponge cakes	346:369	gluten-free sponge cakes	346:369	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	29	theme	ashes	1234:1238	arg1	levels					1193:1198	higher levels	1186:1198	higher levels of moisture, proteins, lipids and ashes	1186:1238	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	1	30	theme	Most	172:175	arg1	formulations					196:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	3	31	theme	raw	683:685	arg1	cakes					687:691	the raw cakes	679:691	the raw cakes	679:691	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	2	32	dep	galactomannan	460:472	arg1	mixed					503:507	mixed	503:507	mixed at 0.5 and 1.0% proportions	503:535	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	2	32	dep	galactomannan	460:472	arg1	isolated					491:498	isolated	491:498	isolated	491:498	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	1	33	theme	gluten-free	177:187	arg1	formulations					196:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	0	34	theme	hydrocolloids	13:25	arg1	Influence					0:8	Influence	0:8	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free	0:123	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	35	theme	gluten-free	113:123	arg1	characteristics					94:108	the physicochemical and sensory characteristics	62:108	the physicochemical and sensory characteristics of gluten-free	62:123	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	6	36	theme	excellent	1300:1308	arg1	option					1315:1320	an excellent food option	1297:1320	an excellent food option without gluten and high nutritional value	1297:1362	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	1	37	theme	bakery	189:194	arg1	formulations					196:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	4	38	theme	air	923:925	arg1	viscosity					873:881	the viscosity	869:881	the viscosity	869:881	Hydrocolloids increased the viscosity, specific gravity, and incorporation of air into the batters.
30456405	4	38	theme	air	923:925	arg1	gravity					893:899	specific gravity	884:899	specific gravity	884:899	Hydrocolloids increased the viscosity, specific gravity, and incorporation of air into the batters.
30456405	4	38	theme	air	923:925	arg1	incorporation					906:918	incorporation	906:918	incorporation of air into the batters	906:942	Hydrocolloids increased the viscosity, specific gravity, and incorporation of air into the batters.
30456405	2	39	theme	substitution	425:436	arg1	effects					402:408	the effects	398:408	the effects of the partial substitution of fava bean flour	398:455	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	40	contain	had	1096:1098	arg1	Cakes					1070:1074	Cakes	1070:1074	Cakes based on fava beans	1070:1094	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	6	40	contain	had	1096:1098	arg2	acceptance					1115:1124	better sensory acceptance	1100:1124	better sensory acceptance	1100:1124	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	6	41	theme	lipids	1223:1228	arg1	levels					1193:1198	higher levels	1186:1198	higher levels of moisture, proteins, lipids and ashes	1186:1238	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	2	42	theme	partial	417:423	arg1	substitution					425:436	the partial substitution	413:436	the partial substitution of fava bean flour	413:455	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	6	43	theme	commercial	1261:1270	arg1	reference					1272:1280	the commercial reference	1257:1280	the commercial reference	1257:1280	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	5	44	theme	Xanthan	945:951	arg1	gum					953:955	Xanthan gum	945:955	Xanthan gum	945:955	Xanthan gum increased the cakes' firmness after cooking; in what concerns color parameters, hydrocolloids did not interfere.
30456405	0	45	theme	xanthan	46:52	arg1	hydrocolloids					13:25	hydrocolloids	13:25	hydrocolloids (galactomannan and xanthan gum)	13:57	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	45	theme	xanthan	46:52	arg1	gum					54:56	xanthan gum	46:56	xanthan gum	46:56	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	46	theme	fava	140:143	arg1	beans					145:149	fava beans	140:149	fava beans (Phaseolus lunatus)	140:169	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	46	theme	fava	140:143	arg1	lunatus					162:168	Phaseolus lunatus	152:168	Phaseolus lunatus	152:168	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	1	47	contain	have	209:212	arg1	formulations					196:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations	172:207	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	1	47	contain	have	209:212	arg2	sources					221:227	starch sources	214:227	starch sources of low nutritional value	214:252	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	2	48	dep	evaluating	387:396	arg1	addition					375:382	addition	375:382	addition	375:382	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	2	49	theme	Phaseolus	305:313	arg1	beans					298:302	fava beans	293:302	fava beans (Phaseolus lunatus)	293:322	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	2	49	theme	Phaseolus	305:313	arg1	lunatus					315:321	Phaseolus lunatus	305:321	Phaseolus lunatus	305:321	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	0	50	theme	Phaseolus	152:160	arg1	beans					145:149	fava beans	140:149	fava beans (Phaseolus lunatus)	140:169	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	50	theme	Phaseolus	152:160	arg1	lunatus					162:168	Phaseolus lunatus	152:168	Phaseolus lunatus	152:168	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	3	51	theme	specific	610:617	arg1	gravity					619:625	specific gravity	610:625	specific gravity	610:625	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	52	theme	specific	783:790	arg1	volume					792:797	specific volume	783:797	specific volume	783:797	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	1	53	theme	starch	214:219	arg1	sources					221:227	starch sources	214:227	starch sources of low nutritional value	214:252	Most gluten-free bakery formulations have starch sources of low nutritional value.
30456405	6	54	theme	moisture	1203:1210	arg1	levels					1193:1198	higher levels	1186:1198	higher levels of moisture, proteins, lipids and ashes	1186:1238	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	3	55	dep	physicochemical	744:758	arg1	color					812:816	color	812:816	color	812:816	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	55	dep	physicochemical	744:758	arg1	texture					800:806	texture	800:806	texture	800:806	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	55	dep	physicochemical	744:758	arg1	composition					770:780	chemical composition	761:780	chemical composition	761:780	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	55	dep	physicochemical	744:758	arg1	volume					792:797	specific volume	783:797	specific volume	783:797	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	0	56	dep	hydrocolloids	13:25	arg1	hydrocolloids					13:25	hydrocolloids	13:25	hydrocolloids (galactomannan and xanthan gum)	13:57	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	56	dep	hydrocolloids	13:25	arg1	galactomannan					28:40	galactomannan	28:40	galactomannan	28:40	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	0	56	dep	hydrocolloids	13:25	arg1	gum					54:56	xanthan gum	46:56	xanthan gum	46:56	Influence of hydrocolloids (galactomannan and xanthan gum) on the physicochemical and sensory characteristics of gluten-free cakes based on fava beans (Phaseolus lunatus).
30456405	3	57	theme	physicochemical	744:758	arg1	properties					833:842	physicochemical (chemical composition, specific volume, texture and color) and sensorial properties	744:842	physicochemical (chemical composition, specific volume, texture and color) and sensorial properties	744:842	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	3	58	theme	chemical	761:768	arg1	composition					770:780	chemical composition	761:780	chemical composition	761:780	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	6	59	theme	better	1100:1105	arg1	acceptance					1115:1124	better sensory acceptance	1100:1124	better sensory acceptance	1100:1124	Cakes based on fava beans had better sensory acceptance with or without the presence of hydrocolloids and presented higher levels of moisture, proteins, lipids and ashes when compared to the commercial reference, proving to be an excellent food option without gluten and high nutritional value.
30456405	3	60	theme	cooked	704:709	arg1	cakes					711:715	the cooked cakes	700:715	the cooked cakes	700:715	The properties such as viscosity, specific gravity and microscopy of the air bubbles were evaluated in the raw cakes, while the cooked cakes were analyzed according to physicochemical (chemical composition, specific volume, texture and color) and sensorial properties.
30456405	2	61	theme	flour	451:455	arg1	substitution					425:436	the partial substitution	413:436	the partial substitution of fava bean flour	413:455	The objective of this work was to use fava beans (Phaseolus lunatus) for the production of gluten-free sponge cakes, in addition to evaluating the effects of the partial substitution of fava bean flour by galactomannan and xanthan gum, isolated or mixed at 0.5 and 1.0% proportions, compared with a commercial reference.
30456405	1	62	theme	low	232:234	arg1	value					248:252	low nutritional value	232:252	low nutritional value	232:252	Most gluten-free bakery formulations have starch sources of low nutritional value.
30093023	8	0	theme	smart	1306:1310	arg1	composites					1312:1321	the developed smart composites	1292:1321	the developed smart composites	1292:1321	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	7	1	theme	GAGs	1184:1187	arg1	expression					1136:1145	a consistent expression	1123:1145	a consistent expression of collagen II	1123:1160	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	7	1	theme	GAGs	1184:1187	arg1	synthesis					1171:1179	high synthesis	1166:1179	high synthesis of GAGs and proteoglycans	1166:1205	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	3	2	theme	infection	539:547	arg1	threat					549:554	infection threat	539:554	infection threat	539:554	However, they frequently lack mechanical features required for cartilage applications and are commonly subjected to infection threat.
30093023	4	3	theme	molecular	615:623	arg1	spacer					625:630	molecular spacer	615:630	molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	615:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	0	4	theme	composite	90:98	arg1	hydrogels					100:108	composite hydrogels	90:108	composite hydrogels	90:108	Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair.
30093023	5	5	theme	epidermidis	943:953	arg1	proliferation					909:921	the proliferation	905:921	the proliferation of S. aureus and S. epidermidis clinical isolates	905:971	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	5	6	theme	clinical	955:962	arg1	isolates					964:971	clinical isolates	955:971	clinical isolates	955:971	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	0	7	theme	cartilage	114:122	arg1	repair					124:129	cartilage repair	114:129	cartilage repair	114:129	Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair.
30093023	8	8	contain	have	1323:1326	arg2	potential					1336:1344	a great potential	1328:1344	a great potential as tools for cartilage tissue engineering	1328:1386	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	8	8	contain	have	1323:1326	arg1	composites					1312:1321	the developed smart composites	1292:1321	the developed smart composites	1292:1321	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	2	9	from	class	322:326	arg1	respect					272:278	this respect	267:278	this respect	267:278	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	10	theme	hyaluronic	816:825	arg1	acid					827:830	hyaluronic acid	816:830	hyaluronic acid	816:830	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	4	11	link	Nature-derived	777:790	arg1	gels					792:795	several Nature-derived gels	769:795	several Nature-derived gels based on chitosan, hyaluronic acid or alginate	769:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	4	12	theme	Nature-derived	777:790	arg1	gels					792:795	several Nature-derived gels	769:795	several Nature-derived gels based on chitosan, hyaluronic acid or alginate	769:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	7	13	theme	matrix	1251:1256	arg1	formation					1228:1236	the formation	1224:1236	the formation of cartilage matrix	1224:1256	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	4	14	theme	several	769:775	arg1	gels					792:795	several Nature-derived gels	769:795	several Nature-derived gels based on chitosan, hyaluronic acid or alginate	769:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	2	15	theme	Nature-derived	281:294	arg1	hydrogels					296:304	Nature-derived hydrogels	281:304	Nature-derived hydrogels	281:304	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	2	15	theme	Nature-derived	281:294	arg1	class					322:326	a promising class	310:326	a promising class of scaffolds	310:339	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	16	theme	Young	739:743	arg1	modulus					747:753	superior compressive Young's modulus	718:753	superior compressive Young's modulus	718:753	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	1	17	theme	clinical	185:192	arg1	Biomaterials					132:143	Biomaterials	132:143	Biomaterials for cartilage repair	132:164	Biomaterials for cartilage repair are still far from clinical requirements, even if several studies recently focused on this topic.
30093023	1	17	theme	clinical	185:192	arg1	requirements					194:205	clinical requirements	185:205	clinical requirements	185:205	Biomaterials for cartilage repair are still far from clinical requirements, even if several studies recently focused on this topic.
30093023	6	18	theme	stem	1043:1046	arg1	cells					1048:1052	human mesenchymal stem cells	1025:1052	human mesenchymal stem cells seeded on the hydrogels	1025:1076	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	7	19	theme	high	1166:1169	arg1	synthesis					1171:1179	high synthesis	1166:1179	high synthesis of GAGs and proteoglycans	1166:1205	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	5	20	theme	honey	868:872	arg1	addition					849:856	The addition	845:856	The addition of Manuka honey	845:872	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	8	21	theme	great	1330:1334	arg1	potential					1336:1344	a great potential	1328:1344	a great potential as tools for cartilage tissue engineering	1328:1386	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	6	22	theme	cytotoxic	990:998	arg1	effects					1000:1006	no cytotoxic effects	987:1006	no cytotoxic effects	987:1006	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	5	23	theme	Manuka	861:866	arg1	honey					868:872	Manuka honey	861:872	Manuka honey	861:872	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	7	24	theme	collagen	1150:1157	arg1	expression					1136:1145	a consistent expression	1123:1145	a consistent expression of collagen II	1123:1160	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	7	24	theme	collagen	1150:1157	arg1	synthesis					1171:1179	high synthesis	1166:1179	high synthesis of GAGs and proteoglycans	1166:1205	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	6	25	theme	mesenchymal	1031:1041	arg1	cells					1048:1052	human mesenchymal stem cells	1025:1052	human mesenchymal stem cells seeded on the hydrogels	1025:1076	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	2	26	theme	cellular	396:403	arg1	microenvironment					405:420	the native cellular microenvironment	385:420	the native cellular microenvironment	385:420	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	0	27	theme	Antibacterial	0:12	arg1	effectiveness					14:26	Antibacterial effectiveness	0:26	Antibacterial effectiveness	0:26	Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair.
30093023	2	28	link	Nature-derived	281:294	arg1	hydrogels					296:304	Nature-derived hydrogels	281:304	Nature-derived hydrogels	281:304	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	2	28	link	Nature-derived	281:294	arg1	class					322:326	a promising class	310:326	a promising class of scaffolds	310:339	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	8	29	theme	developed	1296:1304	arg1	composites					1312:1321	the developed smart composites	1292:1321	the developed smart composites	1292:1321	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	4	30	theme	superior	718:725	arg1	modulus					747:753	superior compressive Young's modulus	718:753	superior compressive Young's modulus	718:753	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	3	31	theme	mechanical	453:462	arg1	features					464:471	mechanical features	453:471	mechanical features required for cartilage applications	453:507	However, they frequently lack mechanical features required for cartilage applications and are commonly subjected to infection threat.
30093023	0	32	theme	improved	34:41	arg1	properties					54:63	improved mechanical properties	34:63	improved mechanical properties	34:63	Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair.
30093023	6	33	theme	human	1025:1029	arg1	cells					1048:1052	human mesenchymal stem cells	1025:1052	human mesenchymal stem cells seeded on the hydrogels	1025:1076	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	2	34	theme	promising	312:320	arg1	hydrogels					296:304	Nature-derived hydrogels	281:304	Nature-derived hydrogels	281:304	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	2	34	theme	promising	312:320	arg1	class					322:326	a promising class	310:326	a promising class of scaffolds	310:339	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	35	theme	intrinsic	679:687	arg1	properties					703:712	intrinsic antibacterial properties	679:712	intrinsic antibacterial properties	679:712	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	1	36	theme	several	216:222	arg1	studies					224:230	several studies	216:230	several studies	216:230	Biomaterials for cartilage repair are still far from clinical requirements, even if several studies recently focused on this topic.
30093023	4	37	theme	Manuka	599:604	arg1	use					592:594	the innovative use	577:594	the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	577:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	7	38	theme	cartilage	1241:1249	arg1	matrix					1251:1256	cartilage matrix	1241:1256	cartilage matrix	1241:1256	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	4	39	with	composites	663:672	arg1	properties					703:712	intrinsic antibacterial properties	679:712	intrinsic antibacterial properties	679:712	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	2	40	theme	native	389:394	arg1	microenvironment					405:420	the native cellular microenvironment	385:420	the native cellular microenvironment	385:420	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	0	41	theme	mechanical	43:52	arg1	properties					54:63	improved mechanical properties	34:63	improved mechanical properties	34:63	Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair.
30093023	7	42	theme	consistent	1125:1134	arg1	expression					1136:1145	a consistent expression	1123:1145	a consistent expression of collagen II	1123:1160	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	5	43	dep	aureus	929:934	arg1	isolates					964:971	clinical isolates	955:971	clinical isolates	955:971	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	3	44	theme	cartilage	486:494	arg1	applications					496:507	cartilage applications	486:507	cartilage applications	486:507	However, they frequently lack mechanical features required for cartilage applications and are commonly subjected to infection threat.
30093023	7	45	theme	proteoglycans	1193:1205	arg1	expression					1136:1145	a consistent expression	1123:1145	a consistent expression of collagen II	1123:1160	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	7	45	theme	proteoglycans	1193:1205	arg1	synthesis					1171:1179	high synthesis	1166:1179	high synthesis of GAGs and proteoglycans	1166:1205	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	2	46	theme	tissue	355:360	arg1	engineering					362:372	cartilage tissue engineering	345:372	cartilage tissue engineering	345:372	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	47	theme	innovative	581:590	arg1	use					592:594	the innovative use	577:594	the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	577:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	2	48	theme	cartilage	345:353	arg1	engineering					362:372	cartilage tissue engineering	345:372	cartilage tissue engineering	345:372	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	49	theme	compressive	727:737	arg1	modulus					747:753	superior compressive Young's modulus	718:753	superior compressive Young's modulus	718:753	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	1	50	theme	cartilage	149:157	arg1	repair					159:164	cartilage repair	149:164	cartilage repair	149:164	Biomaterials for cartilage repair are still far from clinical requirements, even if several studies recently focused on this topic.
30093023	5	51	theme	aureus	929:934	arg1	proliferation					909:921	the proliferation	905:921	the proliferation of S. aureus and S. epidermidis clinical isolates	905:971	The addition of Manuka honey made hydrogels able to inhibit the proliferation of S. aureus and S. epidermidis clinical isolates.
30093023	4	52	theme	gellan	646:651	arg1	composites					663:672	gellan gum-based composites	646:672	gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	646:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	7	53	theme	chondrogenesis	1089:1102	arg1	experiments					1104:1114	chondrogenesis experiments	1089:1114	chondrogenesis experiments	1089:1114	Moreover, chondrogenesis experiments showed a consistent expression of collagen II and high synthesis of GAGs and proteoglycans, thus indicating the formation of cartilage matrix.
30093023	2	54	theme	scaffolds	331:339	arg1	hydrogels					296:304	Nature-derived hydrogels	281:304	Nature-derived hydrogels	281:304	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	2	54	theme	scaffolds	331:339	arg1	class					322:326	a promising class	310:326	a promising class of scaffolds	310:339	In this respect, Nature-derived hydrogels are a promising class of scaffolds for cartilage tissue engineering, mimicking the native cellular microenvironment.
30093023	4	55	theme	gels	792:795	arg1	respect					758:764	respect	758:764	respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	758:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	4	56	from	composites	663:672	arg1	respect					758:764	respect	758:764	respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	758:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	4	57	theme	antibacterial	689:701	arg1	properties					703:712	intrinsic antibacterial properties	679:712	intrinsic antibacterial properties	679:712	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
30093023	6	58	located	detected	1013:1020	arg1	cells					1048:1052	human mesenchymal stem cells	1025:1052	human mesenchymal stem cells seeded on the hydrogels	1025:1076	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	6	58	located	detected	1013:1020	arg2	effects					1000:1006	no cytotoxic effects	987:1006	no cytotoxic effects	987:1006	Furthermore, no cytotoxic effects were detected on human mesenchymal stem cells seeded on the hydrogels.
30093023	8	59	theme	cartilage	1359:1367	arg1	engineering					1376:1386	cartilage tissue engineering	1359:1386	cartilage tissue engineering	1359:1386	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	8	60	theme	tissue	1369:1374	arg1	engineering					1376:1386	cartilage tissue engineering	1359:1386	cartilage tissue engineering	1359:1386	Overall, these data suggest that the developed smart composites have a great potential as tools for cartilage tissue engineering.
30093023	4	61	theme	gum-based	653:661	arg1	composites					663:672	gellan gum-based composites	646:672	gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate	646:842	This work describes the innovative use of Manuka honey as molecular spacer for preparing gellan gum-based composites with intrinsic antibacterial properties and superior compressive Young's modulus in respect of several Nature-derived gels based on chitosan, hyaluronic acid or alginate.
29580414	1	0	theme	release	149:155	arg1	mechanism					157:165	drug release mechanism	144:165	drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials	144:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	2	1	theme	drug	358:361	arg1	xanthan					297:303	xanthan	297:303	xanthan	297:303	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	1	theme	drug	358:361	arg1	gums					320:323	tragacanth gums	309:323	tragacanth gums	309:323	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	1	theme	drug	358:361	arg1	systems					371:377	modified drug release systems	349:377	modified drug release systems	349:377	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	4	2	theme	Korsmeyer-Peppas	733:748	arg1	models					685:690	kinetic models	677:690	kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas	677:748	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	5	3	theme	xanthan	974:980	arg1	gum					982:984	xanthan gum	974:984	xanthan gum	974:984	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	3	4	theme	semi-empirical	527:540	arg1	models					542:547	semi-empirical models	527:547	semi-empirical models	527:547	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	5	dep	Young-Dupree	505:516	arg1	models					542:547	semi-empirical models	527:547	semi-empirical models	527:547	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	4	6	theme	drug	573:576	arg1	release					578:584	the drug release	569:584	the drug release studies	569:592	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	5	7	theme	tragacanth	918:927	arg1	gum					929:931	tragacanth gum	918:931	wherein tragacanth gum	910:931	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	2	8	theme	release	363:369	arg1	xanthan					297:303	xanthan	297:303	xanthan	297:303	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	8	theme	release	363:369	arg1	gums					320:323	tragacanth gums	309:323	tragacanth gums	309:323	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	8	theme	release	363:369	arg1	systems					371:377	modified drug release systems	349:377	modified drug release systems	349:377	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	5	9	theme	processes	872:880	arg1	proportion					805:814	the proportion	801:814	the proportion of biopolymer, surface properties, and the drug release processes	801:880	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	5	10	theme	usual	943:947	arg1	mechanism					957:965	a usual erosion mechanism	941:965	a usual erosion mechanism	941:965	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	1	11	theme	composition	179:189	arg1	tablets					191:197	binary composition tablets	172:197	binary composition tablets formed by quetiapine fumarate and biopolymer materials	172:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	5	12	theme	drug	1050:1053	arg1	release					1035:1041	the release	1031:1041	the release of the drug	1031:1053	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	5	13	dep	affected	900:907	arg1	while					968:972	while	968:972	while	968:972	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	14	theme	release	578:584	arg1	studies					586:592	the drug release studies	569:592	the drug release studies	569:592	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	1	15	theme	binary	172:177	arg1	tablets					191:197	binary composition tablets	172:197	binary composition tablets formed by quetiapine fumarate and biopolymer materials	172:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	0	16	theme	gum-type	8:15	arg1	biopolymers					17:27	Natural gum-type biopolymers	0:27	Natural gum-type biopolymers as potential modified nonpolar drug	0:63	Natural gum-type biopolymers as potential modified nonpolar drug release systems.
29580414	3	17	theme	sessile	424:430	arg1	method					437:442	the sessile drop method	420:442	the sessile drop method	420:442	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	0	18	theme	Natural	0:6	arg1	biopolymers					17:27	Natural gum-type biopolymers	0:27	Natural gum-type biopolymers as potential modified nonpolar drug	0:63	Natural gum-type biopolymers as potential modified nonpolar drug release systems.
29580414	4	19	theme	order	700:704	arg1	models					685:690	kinetic models	677:690	kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas	677:748	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	3	20	theme	drop	432:435	arg1	method					437:442	the sessile drop method	420:442	the sessile drop method	420:442	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	5	21	dep	wherein	910:916	arg1	gum					929:931	tragacanth gum	918:931	wherein tragacanth gum	910:931	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	22	theme	order	713:717	arg1	models					685:690	kinetic models	677:690	kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas	677:748	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	3	23	theme	surface	455:461	arg1	SFE					476:478	SFE	476:478	SFE	476:478	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	23	theme	surface	455:461	arg1	determinate					485:495	determinate	485:495	determinate	485:495	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	23	theme	surface	455:461	arg1	energy					468:473	the surface free energy	451:473	the surface free energy (SFE)	451:479	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	24	theme	surface	384:390	arg1	studies					392:398	The surface studies	380:398	The surface studies	380:398	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	4	25	theme	other	557:561	arg1	hand					563:566	the other hand	553:566	the other hand	553:566	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	2	26	theme	tragacanth	309:318	arg1	xanthan					297:303	xanthan	297:303	xanthan	297:303	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	26	theme	tragacanth	309:318	arg1	gums					320:323	tragacanth gums	309:323	tragacanth gums	309:323	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	26	theme	tragacanth	309:318	arg1	systems					371:377	modified drug release systems	349:377	modified drug release systems	349:377	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	3	27	theme	free	463:466	arg1	SFE					476:478	SFE	476:478	SFE	476:478	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	27	theme	free	463:466	arg1	determinate					485:495	determinate	485:495	determinate	485:495	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	3	27	theme	free	463:466	arg1	energy					468:473	the surface free energy	451:473	the surface free energy (SFE)	451:479	The surface studies were carried out by the sessile drop method, while the surface free energy (SFE) was determinate through Young-Dupree and OWRK semi-empirical models.
29580414	0	28	theme	modified	42:49	arg1	drug					60:63	potential modified nonpolar drug	32:63	potential modified nonpolar drug	32:63	Natural gum-type biopolymers as potential modified nonpolar drug release systems.
29580414	5	29	theme	drug	859:862	arg1	processes					872:880	the drug release processes	855:880	the drug release processes	855:880	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	30	theme	dissolution	621:631	arg1	tests					633:637	in vitro dissolution tests	612:637	in vitro dissolution tests	612:637	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	1	31	theme	quetiapine	209:218	arg1	fumarate					220:227	quetiapine fumarate and biopolymer materials	209:252	fumarate	220:227	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	0	32	theme	potential	32:40	arg1	drug					60:63	potential modified nonpolar drug	32:63	potential modified nonpolar drug	32:63	Natural gum-type biopolymers as potential modified nonpolar drug release systems.
29580414	5	33	theme	biopolymer	819:828	arg1	proportion					805:814	the proportion	801:814	the proportion of biopolymer, surface properties, and the drug release processes	801:880	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	34	dep	in	612:613	arg1	vitro					615:619	vitro	615:619	vitro	615:619	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	2	35	theme	modified	349:356	arg1	xanthan					297:303	xanthan	297:303	xanthan	297:303	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	35	theme	modified	349:356	arg1	gums					320:323	tragacanth gums	309:323	tragacanth gums	309:323	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	2	35	theme	modified	349:356	arg1	systems					371:377	modified drug release systems	349:377	modified drug release systems	349:377	The biopolymers correspond to xanthan and tragacanth gums, which are projected as modified drug release systems.
29580414	0	36	theme	nonpolar	51:58	arg1	drug					60:63	potential modified nonpolar drug	32:63	potential modified nonpolar drug	32:63	Natural gum-type biopolymers as potential modified nonpolar drug release systems.
29580414	4	37	theme	kinetic	677:683	arg1	models					685:690	kinetic models	677:690	kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas	677:748	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	1	38	theme	surface	121:127	arg1	properties					129:138	surface properties	121:138	surface properties	121:138	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	5	39	theme	release	864:870	arg1	processes					872:880	the drug release processes	855:880	the drug release processes	855:880	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	5	40	theme	swelling	998:1005	arg1	mechanism					1007:1015	a swelling mechanism	996:1015	a swelling mechanism that controls the release of the drug	996:1053	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	41	theme	Higuchi	720:726	arg1	models					685:690	kinetic models	677:690	kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas	677:748	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	4	42	theme	first	707:711	arg1	order					713:717	first order	707:717	first order	707:717	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	5	43	theme	properties	839:848	arg1	proportion					805:814	the proportion	801:814	the proportion of biopolymer, surface properties, and the drug release processes	801:880	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	1	44	theme	biopolymer	233:242	arg1	materials					244:252	quetiapine fumarate and biopolymer materials	209:252	materials	244:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	5	45	theme	erosion	949:955	arg1	mechanism					957:965	a usual erosion mechanism	941:965	a usual erosion mechanism	941:965	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	1	46	from	tablets	191:197	arg1	relationship					100:111	the relationship	96:111	the relationship between surface properties	96:138	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	1	46	from	tablets	191:197	arg1	mechanism					157:165	drug release mechanism	144:165	drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials	144:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29580414	5	47	theme	surface	831:837	arg1	properties					839:848	surface properties	831:848	surface properties	831:848	The results showed that depending on the type and the proportion of biopolymer, surface properties, and the drug release processes are significantly affected, wherein tragacanth gum present a usual erosion mechanism, while xanthan gum describes a swelling mechanism that controls the release of the drug.
29580414	4	48	theme	in	612:613	arg1	tests					633:637	in vitro dissolution tests	612:637	in vitro dissolution tests	612:637	On the other hand, the drug release studies were performed by in vitro dissolution tests, where the data were analyzed through kinetic models of zero order, first order, Higuchi, and Korsmeyer-Peppas.
29580414	1	49	theme	drug	144:147	arg1	mechanism					157:165	drug release mechanism	144:165	drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials	144:252	In this work, the relationship between surface properties and drug release mechanism from binary composition tablets formed by quetiapine fumarate and biopolymer materials was studied.
29309869	0	0	from	anti-hyperglycaemia	15:33	arg1	SI-04					118:122	Pleurotus eryngii SI-04	100:122	Pleurotus eryngii SI-04	100:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	2	1	theme	serum	584:588	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	0	2	theme	Pleurotus	100:108	arg1	SI-04					118:122	Pleurotus eryngii SI-04	100:122	Pleurotus eryngii SI-04	100:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	1	3	from	anti-hyperglycaemia	175:193	arg1	broth					289:293	the broth	285:293	the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	285:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	4	with	mice	341:344	arg1	nephropathy					389:399	streptozotocin (STZ)-induced diabetic nephropathy	351:399	streptozotocin (STZ)-induced diabetic nephropathy (DN)	351:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	0	5	from	Antioxidation	0:12	arg1	SI-04					118:122	Pleurotus eryngii SI-04	100:122	Pleurotus eryngii SI-04	100:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	2	6	theme	VLDL-C	605:610	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	3	7	theme	potential	804:812	arg1	effects					867:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	2	8	theme	antioxidant	647:657	arg1	status					659:664	renal antioxidant status	641:664	renal antioxidant status (GSH-Px, SOD, CAT and MDA)	641:691	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	9	dep	GSH-Px	667:672	arg1	MDA					688:690	MDA	688:690	MDA	688:690	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	9	dep	GSH-Px	667:672	arg1	CAT					680:682	CAT	680:682	CAT	680:682	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	9	dep	GSH-Px	667:672	arg1	SOD					675:677	SOD	675:677	SOD	675:677	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	1	10	theme	Pleurotus	298:306	arg1	SI-04					316:320	Pleurotus eryngii SI-04	298:320	Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	298:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	11	from	mice	341:344	arg1	broth					289:293	the broth	285:293	the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	285:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	4	12	theme	polysaccharides	1058:1072	arg1	compositions					1017:1028	monosaccharide compositions	1002:1028	monosaccharide compositions	1002:1028	In addition, the monosaccharide compositions and bond types of these two polysaccharides were analysed.
29309869	4	12	theme	polysaccharides	1058:1072	arg1	types					1039:1043	bond types	1034:1043	bond types	1034:1043	In addition, the monosaccharide compositions and bond types of these two polysaccharides were analysed.
29309869	2	13	theme	TG	601:602	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	3	14	used	used	941:944	arg2	drug					979:982	natural drug	971:982	natural drug	971:982	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	14	used	used	941:944	arg2	SI-04					926:930	P. eryngii SI-04	915:930	P. eryngii SI-04	915:930	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	14	used	used	941:944	arg2	food					962:965	a functional food	949:965	a functional food	949:965	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	15	theme	P.	915:916	arg1	SI-04					926:930	P. eryngii SI-04	915:930	P. eryngii SI-04	915:930	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	15	theme	P.	915:916	arg1	food					962:965	a functional food	949:965	a functional food	949:965	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	15	theme	P.	915:916	arg1	drug					979:982	natural drug	971:982	natural drug	971:982	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	0	16	from	effects	54:60	arg1	SI-04					118:122	Pleurotus eryngii SI-04	100:122	Pleurotus eryngii SI-04	100:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	2	17	theme	TC	597:598	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	3	18	theme	natural	971:977	arg1	SI-04					926:930	P. eryngii SI-04	915:930	P. eryngii SI-04	915:930	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	18	theme	natural	971:977	arg1	drug					979:982	natural drug	971:982	natural drug	971:982	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	19	theme	anti-hyperglycaemic	827:845	arg1	effects					867:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	2	20	theme	GLU	522:524	arg1	levels					526:531	GLU levels	522:531	GLU levels	522:531	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	21	theme	potential	460:468	arg1	effects					478:484	potential anti-DN effects	460:484	potential anti-DN effects	460:484	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	1	22	theme	diabetic	380:387	arg1	nephropathy					389:399	streptozotocin (STZ)-induced diabetic nephropathy	351:399	streptozotocin (STZ)-induced diabetic nephropathy (DN)	351:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	4	23	dep	compositions	1017:1028	arg1	the					998:1000	the	998:1000	the	998:1000	In addition, the monosaccharide compositions and bond types of these two polysaccharides were analysed.
29309869	1	24	theme	renoprotective	214:227	arg1	effects					229:235	renoprotective effects	214:235	renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	214:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	3	25	theme	eryngii	918:924	arg1	SI-04					926:930	P. eryngii SI-04	915:930	P. eryngii SI-04	915:930	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	25	theme	eryngii	918:924	arg1	food					962:965	a functional food	949:965	a functional food	949:965	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	25	theme	eryngii	918:924	arg1	drug					979:982	natural drug	971:982	natural drug	971:982	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	1	26	from	SI-04	316:320	arg1	mice					341:344	mice	341:344	mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	341:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	0	27	dep	Pleurotus	100:108	arg1	eryngii					110:116	eryngii	110:116	eryngii	110:116	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	1	28	theme	SI-04	316:320	arg1	broth					289:293	the broth	285:293	the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	285:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	2	29	theme	LDL-C	616:620	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	4	30	theme	bond	1034:1037	arg1	types					1039:1043	bond types	1034:1043	bond types	1034:1043	In addition, the monosaccharide compositions and bond types of these two polysaccharides were analysed.
29309869	1	31	theme	present	129:135	arg1	study					137:141	The present study	125:141	The present study	125:141	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	2	32	dep	status	659:664	arg1	GSH-Px					667:672	GSH-Px	667:672	GSH-Px	667:672	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	33	theme	anti-DN	470:476	arg1	effects					478:484	potential anti-DN effects	460:484	potential anti-DN effects	460:484	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	3	34	theme	renoprotective	852:865	arg1	effects					867:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	1	35	theme	extracellular	244:256	arg1	EPS					275:277	EPS	275:277	EPS	275:277	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	35	theme	extracellular	244:256	arg1	polysaccharides					258:272	two extracellular polysaccharides	240:272	two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	240:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	0	36	theme	renoprotective	39:52	arg1	effects					54:60	renoprotective effects	39:60	renoprotective effects	39:60	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	1	37	from	broth	289:293	arg1	effects					229:235	renoprotective effects	214:235	renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	214:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	37	from	broth	289:293	arg1	EPS					275:277	EPS	275:277	EPS	275:277	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	37	from	broth	289:293	arg1	anti-hyperglycaemia					175:193	the anti-hyperglycaemia	171:193	the anti-hyperglycaemia	171:193	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	37	from	broth	289:293	arg1	mice					341:344	mice	341:344	mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	341:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	37	from	broth	289:293	arg1	polysaccharides					258:272	two extracellular polysaccharides	240:272	two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	240:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	37	from	broth	289:293	arg1	hypolipidemia					196:208	hypolipidemia	196:208	hypolipidemia	196:208	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	38	theme	polysaccharides	258:272	arg1	hypolipidemia					196:208	hypolipidemia	196:208	hypolipidemia	196:208	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	38	theme	polysaccharides	258:272	arg1	effects					229:235	renoprotective effects	214:235	renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	214:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	38	theme	polysaccharides	258:272	arg1	anti-hyperglycaemia					175:193	the anti-hyperglycaemia	171:193	the anti-hyperglycaemia	171:193	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	0	39	theme	extracellular	65:77	arg1	polysaccharides					79:93	extracellular polysaccharides	65:93	extracellular polysaccharides from Pleurotus eryngii SI-04	65:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	2	40	theme	UA	564:565	arg1	levels					567:572	UA levels	564:572	UA levels	564:572	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	1	41	theme	-induced	371:378	arg1	nephropathy					389:399	streptozotocin (STZ)-induced diabetic nephropathy	351:399	streptozotocin (STZ)-induced diabetic nephropathy (DN)	351:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	42	dep	SI-04	316:320	arg1	EPS2					332:335	EPS2	332:335	EPS2	332:335	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	1	42	dep	SI-04	316:320	arg1	EPS1					323:326	EPS1	323:326	EPS1	323:326	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	2	43	theme	renal	641:645	arg1	status					659:664	renal antioxidant status	641:664	renal antioxidant status (GSH-Px, SOD, CAT and MDA)	641:691	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	4	44	theme	monosaccharide	1002:1015	arg1	compositions					1017:1028	monosaccharide compositions	1002:1028	monosaccharide compositions	1002:1028	In addition, the monosaccharide compositions and bond types of these two polysaccharides were analysed.
29309869	1	45	dep	Pleurotus	298:306	arg1	eryngii					308:314	eryngii	308:314	eryngii	308:314	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	3	46	theme	functional	951:960	arg1	SI-04					926:930	P. eryngii SI-04	915:930	P. eryngii SI-04	915:930	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	3	46	theme	functional	951:960	arg1	food					962:965	a functional food	949:965	a functional food	949:965	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
29309869	2	47	theme	lipid	590:594	arg1	levels					623:628	serum lipid (TC, TG, VLDL-C and LDL-C) levels	584:628	levels	623:628	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	48	dep	including	487:495	arg1	decreasing					511:520	decreasing	511:520	including significantly decreasing GLU levels	487:531	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	2	49	theme	nephropathy	723:733	arg1	damage					735:740	pathological nephropathy damage	710:740	pathological nephropathy damage	710:740	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	0	50	from	SI-04	118:122	arg1	anti-hyperglycaemia					15:33	anti-hyperglycaemia	15:33	anti-hyperglycaemia	15:33	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	0	50	from	SI-04	118:122	arg1	effects					54:60	renoprotective effects	39:60	renoprotective effects	39:60	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	0	50	from	SI-04	118:122	arg1	Antioxidation					0:12	Antioxidation	0:12	Antioxidation	0:12	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	0	50	from	SI-04	118:122	arg1	polysaccharides					79:93	extracellular polysaccharides	65:93	extracellular polysaccharides from Pleurotus eryngii SI-04	65:122	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	1	51	from	effects	229:235	arg1	broth					289:293	the broth	285:293	the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	285:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	2	52	theme	pathological	710:721	arg1	damage					735:740	pathological nephropathy damage	710:740	pathological nephropathy damage	710:740	The results showed that both EPS1 and EPS2 exhibited potential anti-DN effects, including significantly decreasing GLU levels; suppressing ALB, BUN, CRE and UA levels; reducing serum lipid (TC, TG, VLDL-C and LDL-C) levels; improving renal antioxidant status (GSH-Px, SOD, CAT and MDA); and attenuating pathological nephropathy damage.
29309869	1	53	dep	nephropathy	389:399	arg1	DN					402:403	DN	402:403	DN	402:403	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	0	54	theme	polysaccharides	79:93	arg1	anti-hyperglycaemia					15:33	anti-hyperglycaemia	15:33	anti-hyperglycaemia	15:33	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	0	54	theme	polysaccharides	79:93	arg1	effects					54:60	renoprotective effects	39:60	renoprotective effects	39:60	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	0	54	theme	polysaccharides	79:93	arg1	Antioxidation					0:12	Antioxidation	0:12	Antioxidation	0:12	Antioxidation, anti-hyperglycaemia and renoprotective effects of extracellular polysaccharides from Pleurotus eryngii SI-04.
29309869	1	55	from	hypolipidemia	196:208	arg1	broth					289:293	the broth	285:293	the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN)	285:404	The present study was designed to investigate the anti-hyperglycaemia, hypolipidemia and renoprotective effects of two extracellular polysaccharides (EPS) from the broth of Pleurotus eryngii SI-04 (EPS1 and EPS2) in mice with streptozotocin (STZ)-induced diabetic nephropathy (DN).
29309869	3	56	theme	antioxidant	814:824	arg1	effects					867:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	potential antioxidant, anti-hyperglycaemic, and renoprotective effects	804:873	These results demonstrated that both EPS1 and EPS2 exhibited potential antioxidant, anti-hyperglycaemic, and renoprotective effects against STZ-induced DN, indicating that P. eryngii SI-04 could be used as a functional food and natural drug.
30806622	6	0	located	detected	1145:1152	arg2	aureus					1073:1078	Staphylococcus aureus	1058:1078	Staphylococcus aureus	1058:1078	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	0	located	detected	1145:1152	arg2	albicans					1113:1120	Candida albicans	1105:1120	Candida albicans	1105:1120	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	0	located	detected	1145:1152	arg2	aeruginosa					1093:1102	Pseudomonas aeruginosa	1081:1102	Pseudomonas aeruginosa	1081:1102	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	0	located	detected	1145:1152	arg1	cream					1174:1178	the seaweed mask cream	1157:1178	the seaweed mask cream	1157:1178	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	0	located	detected	1145:1152	arg2	metals					1133:1138	heavy metals	1127:1138	heavy metals	1127:1138	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	0	located	detected	1145:1152	arg2	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	5	1	theme	seaweed	891:897	arg1	spread					983:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	7	2	theme	cosmetic	1415:1422	arg1	products					1424:1431	the cosmetic products	1411:1431	the cosmetic products with anti-aging and moisturizing effects	1411:1472	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	3	theme	Ulva	514:517	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	3	theme	Ulva	514:517	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	3	theme	Ulva	514:517	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	3	theme	Ulva	514:517	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	3	theme	Ulva	514:517	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	4	theme	adhesive	994:1001	arg1	abilities					1003:1011	adhesive abilities	994:1011	adhesive abilities	994:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	1	5	theme	functional	148:157	arg1	ingredient					159:168	functional ingredient	148:168	functional ingredient for cosmetic applications	148:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	1	5	theme	functional	148:157	arg1	compounds					111:119	natural bioactive compounds	93:119	natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications	93:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	4	6	with	protein	587:593	arg1	retention					692:700	moisture retention	683:700	moisture retention	683:700	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	6	with	protein	587:593	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	6	with	protein	587:593	arg1	antibacterial					648:660	high antibacterial	643:660	high antibacterial	643:660	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	6	with	protein	587:593	arg1	proliferation					668:680	cell proliferation	663:680	cell proliferation	663:680	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	7	7	theme	No.	1300:1302	arg1	2011					1337:1340	2011	1337:1340	2011 of the Vietnam Ministry of Health	1337:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	7	theme	No.	1300:1302	arg1	06/2011/TT-BYT					1304:1317	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	8	theme	Ministry	1357:1364	arg1	2011					1337:1340	2011	1337:1340	2011 of the Vietnam Ministry of Health	1337:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	8	theme	Ministry	1357:1364	arg1	06/2011/TT-BYT					1304:1317	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	2	9	theme	seaweeds	288:295	arg1	bioactivities					268:280	bioactivities	268:280	bioactivities	268:280	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	2	9	theme	seaweeds	288:295	arg1	compositions					251:262	the biochemical compositions	235:262	the biochemical compositions	235:262	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	2	10	theme	coastal	312:318	arg1	waters					320:325	coastal waters	312:325	coastal waters of Vietnam	312:336	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	5	11	theme	homogeneous	912:922	arg1	spread					983:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	7	12	theme	seaweed	1185:1191	arg1	mask					1199:1202	The seaweed cream mask	1181:1202	The seaweed cream mask	1181:1202	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	12	theme	seaweed	1185:1191	arg1	safe					1208:1211	safe	1208:1211	safe	1208:1211	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	13	with	products	1424:1431	arg1	effects					1466:1472	anti-aging and moisturizing effects	1438:1472	anti-aging and moisturizing effects	1438:1472	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	14	theme	protein	587:593	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	14	theme	protein	587:593	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	14	theme	protein	587:593	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	14	theme	protein	587:593	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	14	theme	protein	587:593	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	1	15	theme	bioactive	101:109	arg1	ingredient					159:168	functional ingredient	148:168	functional ingredient for cosmetic applications	148:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	1	15	theme	bioactive	101:109	arg1	compounds					111:119	natural bioactive compounds	93:119	natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications	93:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	7	16	theme	human	1248:1252	arg1	skin					1254:1257	normal human skin	1241:1257	normal human skin	1241:1257	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	5	17	theme	storage	947:953	arg1	months					937:942	up to 12 months	928:942	up to 12 months of storage	928:953	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	3	18	theme	seaweeds	412:419	arg1	mixture					401:407	mixture	401:407	mixture of seaweeds extracted with water	401:440	The present study also prepared and evaluated cream mask from mixture of seaweeds extracted with water.
30806622	6	19	theme	aerobic	1023:1029	arg1	albicans					1113:1120	Candida albicans	1105:1120	Candida albicans	1105:1120	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	19	theme	aerobic	1023:1029	arg1	metals					1133:1138	heavy metals	1127:1138	heavy metals	1127:1138	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	19	theme	aerobic	1023:1029	arg1	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	19	theme	aerobic	1023:1029	arg1	aureus					1073:1078	Staphylococcus aureus	1058:1078	Staphylococcus aureus	1058:1078	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	19	theme	aerobic	1023:1029	arg1	aeruginosa					1093:1102	Pseudomonas aeruginosa	1081:1102	Pseudomonas aeruginosa	1081:1102	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	3	20	theme	present	343:349	arg1	study					351:355	The present study	339:355	The present study	339:355	The present study also prepared and evaluated cream mask from mixture of seaweeds extracted with water.
30806622	7	21	theme	Health	1369:1374	arg1	Ministry					1357:1364	the Vietnam Ministry	1345:1364	the Vietnam Ministry of Health	1345:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	22	theme	Vietnam	1349:1355	arg1	Ministry					1357:1364	the Vietnam Ministry	1345:1364	the Vietnam Ministry of Health	1345:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	6	23	theme	mesophilic	1031:1040	arg1	albicans					1113:1120	Candida albicans	1105:1120	Candida albicans	1105:1120	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	23	theme	mesophilic	1031:1040	arg1	metals					1133:1138	heavy metals	1127:1138	heavy metals	1127:1138	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	23	theme	mesophilic	1031:1040	arg1	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	23	theme	mesophilic	1031:1040	arg1	aureus					1073:1078	Staphylococcus aureus	1058:1078	Staphylococcus aureus	1058:1078	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	23	theme	mesophilic	1031:1040	arg1	aeruginosa					1093:1102	Pseudomonas aeruginosa	1081:1102	Pseudomonas aeruginosa	1081:1102	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	24	theme	total	1017:1021	arg1	albicans					1113:1120	Candida albicans	1105:1120	Candida albicans	1105:1120	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	24	theme	total	1017:1021	arg1	metals					1133:1138	heavy metals	1127:1138	heavy metals	1127:1138	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	24	theme	total	1017:1021	arg1	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	24	theme	total	1017:1021	arg1	aureus					1073:1078	Staphylococcus aureus	1058:1078	Staphylococcus aureus	1058:1078	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	24	theme	total	1017:1021	arg1	aeruginosa					1093:1102	Pseudomonas aeruginosa	1081:1102	Pseudomonas aeruginosa	1081:1102	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	5	25	theme	extracts	812:819	arg1	mixture					787:793	a mixture	785:793	a mixture of these seaweed extracts	785:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	26	theme	potential	561:569	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	26	theme	potential	561:569	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	26	theme	potential	561:569	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	26	theme	potential	561:569	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	26	theme	potential	561:569	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	1	27	theme	rich	78:81	arg1	source					83:88	rich source	78:88	rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications	78:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	7	28	theme	cosmetic	1387:1394	arg1	management					1396:1405	cosmetic management	1387:1405	cosmetic management	1387:1405	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	1	29	theme	cosmetic	174:181	arg1	applications					183:194	cosmetic applications	174:194	cosmetic applications	174:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	3	30	theme	cream	385:389	arg1	mask					391:394	cream mask	385:394	cream mask	385:394	The present study also prepared and evaluated cream mask from mixture of seaweeds extracted with water.
30806622	7	31	theme	06/2011/TT-BYT	1304:1317	arg1	provisions					1277:1286	provisions	1277:1286	provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health	1277:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	32	theme	anti-aging	1438:1447	arg1	effects					1466:1472	anti-aging and moisturizing effects	1438:1472	anti-aging and moisturizing effects	1438:1472	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	33	theme	cell	663:666	arg1	proliferation					668:680	cell proliferation	663:680	cell proliferation	663:680	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	34	theme	stable	900:905	arg1	spread					983:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread	891:988	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	6	35	theme	Candida	1105:1111	arg1	albicans					1113:1120	Candida albicans	1105:1120	Candida albicans	1105:1120	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	35	theme	Candida	1105:1111	arg1	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	7	36	theme	dated	1319:1323	arg1	2011					1337:1340	2011	1337:1340	2011 of the Vietnam Ministry of Health	1337:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	36	theme	dated	1319:1323	arg1	06/2011/TT-BYT					1304:1317	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	37	theme	vitamins	629:636	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	37	theme	vitamins	629:636	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	37	theme	vitamins	629:636	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	37	theme	vitamins	629:636	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	37	theme	vitamins	629:636	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	7	38	theme	Circular	1291:1298	arg1	2011					1337:1340	2011	1337:1340	2011 of the Vietnam Ministry of Health	1337:1374	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	38	theme	Circular	1291:1298	arg1	06/2011/TT-BYT					1304:1317	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	Circular No. 06/2011/TT-BYT dated January 25	1291:1334	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	39	theme	rich	571:574	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	39	theme	rich	571:574	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	39	theme	rich	571:574	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	39	theme	rich	571:574	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	39	theme	rich	571:574	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	6	40	theme	heavy	1127:1131	arg1	metals					1133:1138	heavy metals	1127:1138	heavy metals	1127:1138	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	6	40	theme	heavy	1127:1131	arg1	microorganisms					1042:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms	1014:1055	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	5	41	from	analysis	757:764	arg1	mixture					787:793	a mixture	785:793	a mixture of these seaweed extracts	785:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	1	42	theme	natural	93:99	arg1	ingredient					159:168	functional ingredient	148:168	functional ingredient for cosmetic applications	148:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	1	42	theme	natural	93:99	arg1	compounds					111:119	natural bioactive compounds	93:119	natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications	93:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	5	43	theme	yellowish	842:850	arg1	brown					852:856	yellowish brown	842:856	yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	842:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	0	44	theme	Mask	36:39	arg1	Evaluation					16:25	Evaluation	16:25	Evaluation	16:25	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	0	44	theme	Mask	36:39	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	2	45	theme	Vietnam	330:336	arg1	waters					320:325	coastal waters	312:325	coastal waters of Vietnam	312:336	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	5	46	theme	6.1	969:971	arg1	pH					963:964	a pH	961:964	a pH of 6.1	961:971	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	5	47	theme	specific	874:881	arg1	odor					883:886	a specific odor	872:886	a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	872:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	48	theme	high	643:646	arg1	antibacterial					648:660	high antibacterial	643:660	high antibacterial	643:660	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	0	49	theme	Cream	30:34	arg1	Mask					36:39	Cream Mask	30:39	Cream Mask	30:39	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	5	50	theme	Physicochemical	741:755	arg1	analysis					757:764	Physicochemical analysis	741:764	Physicochemical analysis of cream mask from a mixture of these seaweed extracts	741:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	51	with	carotenoids	612:622	arg1	retention					692:700	moisture retention	683:700	moisture retention	683:700	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	51	with	carotenoids	612:622	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	51	with	carotenoids	612:622	arg1	antibacterial					648:660	high antibacterial	643:660	high antibacterial	643:660	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	51	with	carotenoids	612:622	arg1	proliferation					668:680	cell proliferation	663:680	cell proliferation	663:680	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	52	from	brown	852:856	arg1	color					861:865	color	861:865	color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	861:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	7	53	theme	cream	1193:1197	arg1	mask					1199:1202	The seaweed cream mask	1181:1202	The seaweed cream mask	1181:1202	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	7	53	theme	cream	1193:1197	arg1	safe					1208:1211	safe	1208:1211	safe	1208:1211	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	0	54	theme	Vietnamese	46:55	arg1	Seaweeds					57:64	Vietnamese Seaweeds	46:64	Vietnamese Seaweeds	46:64	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	0	55	from	Seaweeds	57:64	arg1	Evaluation					16:25	Evaluation	16:25	Evaluation	16:25	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	0	55	from	Seaweeds	57:64	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Evaluation of Cream Mask from Vietnamese Seaweeds.
30806622	2	56	theme	study	213:217	arg1	aim					201:203	The aim	197:203	The aim of this study	197:217	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	5	57	with	color	861:865	arg1	odor					883:886	a specific odor	872:886	a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	872:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	58	theme	tyrosinase	707:716	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	59	theme	seaweed	804:810	arg1	extracts					812:819	these seaweed extracts	798:819	these seaweed extracts	798:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	5	60	theme	spread	983:988	arg1	odor					883:886	a specific odor	872:886	a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	872:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	61	with	polysaccharide	596:609	arg1	retention					692:700	moisture retention	683:700	moisture retention	683:700	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	61	with	polysaccharide	596:609	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	61	with	polysaccharide	596:609	arg1	antibacterial					648:660	high antibacterial	643:660	high antibacterial	643:660	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	61	with	polysaccharide	596:609	arg1	proliferation					668:680	cell proliferation	663:680	cell proliferation	663:680	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	1	62	theme	compounds	111:119	arg1	source					83:88	rich source	78:88	rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications	78:194	Seaweed is rich source of natural bioactive compounds that could be exploited as functional ingredient for cosmetic applications.
30806622	6	63	theme	mask	1169:1172	arg1	cream					1174:1178	the seaweed mask cream	1157:1178	the seaweed mask cream	1157:1178	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	4	64	dep	Sargassum	490:498	arg1	crassifolium					500:511	Sargassum crassifolium	490:511	Sargassum crassifolium	490:511	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	65	from	mixture	787:793	arg1	analysis					757:764	Physicochemical analysis	741:764	Physicochemical analysis of cream mask from a mixture of these seaweed extracts	741:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	5	65	from	mixture	787:793	arg1	mask					775:778	cream mask	769:778	cream mask from a mixture of these seaweed extracts	769:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	6	66	theme	seaweed	1161:1167	arg1	cream					1174:1178	the seaweed mask cream	1157:1178	the seaweed mask cream	1157:1178	No total aerobic mesophilic microorganisms, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and heavy metals were detected in the seaweed mask cream.
30806622	4	67	with	vitamins	629:636	arg1	retention					692:700	moisture retention	683:700	moisture retention	683:700	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	67	with	vitamins	629:636	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	67	with	vitamins	629:636	arg1	antibacterial					648:660	high antibacterial	643:660	high antibacterial	643:660	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	67	with	vitamins	629:636	arg1	proliferation					668:680	cell proliferation	663:680	cell proliferation	663:680	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	2	68	theme	biochemical	239:249	arg1	compositions					251:262	the biochemical compositions	235:262	the biochemical compositions	235:262	The aim of this study was to evaluate the biochemical compositions and bioactivities of 10 seaweeds collected from coastal waters of Vietnam.
30806622	4	69	theme	inhibitory	718:727	arg1	activities					729:738	tyrosinase inhibitory activities	707:738	tyrosinase inhibitory activities	707:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	70	theme	polysaccharide	596:609	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	70	theme	polysaccharide	596:609	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	70	theme	polysaccharide	596:609	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	70	theme	polysaccharide	596:609	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	70	theme	polysaccharide	596:609	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	5	71	theme	cream	769:773	arg1	mask					775:778	cream mask	769:778	cream mask from a mixture of these seaweed extracts	769:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	5	72	theme	abilities	1003:1011	arg1	odor					883:886	a specific odor	872:886	a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities	872:1011	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	7	73	theme	normal	1241:1246	arg1	skin					1254:1257	normal human skin	1241:1257	normal human skin	1241:1257	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	4	74	theme	moisture	683:690	arg1	retention					692:700	moisture retention	683:700	moisture retention	683:700	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	7	75	dep	06/2011/TT-BYT	1304:1317	arg1	January					1325:1331	January	1325:1331	January	1325:1331	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	5	76	theme	mask	775:778	arg1	analysis					757:764	Physicochemical analysis	741:764	Physicochemical analysis of cream mask from a mixture of these seaweed extracts	741:819	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	77	dep	Kappaphycus	535:545	arg1	alvarezii					547:555	Kappaphycus alvarezii	535:555	Kappaphycus alvarezii	535:555	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	7	78	theme	moisturizing	1453:1464	arg1	effects					1466:1472	anti-aging and moisturizing effects	1438:1472	anti-aging and moisturizing effects	1438:1472	The seaweed cream mask was safe and caused no irritation to normal human skin, and it satisfied provisions of Circular No. 06/2011/TT-BYT dated January 25, 2011 of the Vietnam Ministry of Health, providing cosmetic management for the cosmetic products with anti-aging and moisturizing effects.
30806622	5	79	dep	12	934:935	arg1	to					931:932	to	931:932	to	931:932	Physicochemical analysis of cream mask from a mixture of these seaweed extracts indicated that it is yellowish brown in color with a specific odor of seaweed, stable, and homogeneous for up to 12 months of storage, with a pH of 6.1, and high spread and adhesive abilities.
30806622	4	80	theme	carotenoids	612:622	arg1	lentillifera					476:487	lentillifera	476:487	lentillifera	476:487	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	80	theme	carotenoids	612:622	arg1	sources					576:582	potential rich sources	561:582	potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities	561:738	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	80	theme	carotenoids	612:622	arg1	Kappaphycus					535:545	Kappaphycus	535:545	Kappaphycus	535:545	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	80	theme	carotenoids	612:622	arg1	reticulata					519:528	Ulva reticulata	514:528	Ulva reticulata	514:528	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30806622	4	80	theme	carotenoids	612:622	arg1	Sargassum					490:498	Sargassum	490:498	Sargassum	490:498	The results showed that Caulerpa lentillifera, Sargassum crassifolium, Ulva reticulata, and Kappaphycus alvarezii are potential rich sources of protein, polysaccharide, carotenoids, and vitamins with high antibacterial, cell proliferation, moisture retention, and tyrosinase inhibitory activities.
30879663	0	0	theme	flaxseed	85:92	arg1	gum					94:96	flaxseed gum	85:96	flaxseed gum	85:96	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	1	1	theme	different	139:147	arg1	temperatures					149:160	different temperatures	139:160	different temperatures (25, 40, and 60 °C)	139:180	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	3	2	from	substituents	486:497	arg1	chain					513:517	the xylose chain	502:517	the xylose chain	502:517	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	6	3	theme	G	974:974	arg1	increase					957:964	an increase	954:964	an increase of both G' and G"	954:982	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	0	4	from	Effect	0:5	arg1	behavior					48:55	rheological behavior	36:55	rheological behavior	36:55	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	0	4	from	Effect	0:5	arg1	capacity					73:80	antioxidant capacity	61:80	antioxidant capacity	61:80	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	7	5	theme	elastic	1067:1073	arg1	properties					1075:1084	higher viscous and elastic properties	1048:1084	properties	1075:1084	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	3	6	theme	substituents	486:497	arg1	reduction					473:481	a reduction	471:481	a reduction of substituents on the xylose chain	471:517	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	3	7	theme	xylose	506:511	arg1	chain					513:517	the xylose chain	502:517	the xylose chain	502:517	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	0	8	theme	gum	94:96	arg1	behavior					48:55	rheological behavior	36:55	rheological behavior	36:55	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	0	8	theme	gum	94:96	arg1	capacity					73:80	antioxidant capacity	61:80	antioxidant capacity	61:80	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	4	9	theme	phenolic	580:587	arg1	compounds					589:597	The phenolic compounds	576:597	The phenolic compounds	576:597	The phenolic compounds were also affected by the extraction temperature, influencing the antioxidant capacity of the gum.
30879663	6	10	theme	viscous	876:882	arg1	character					884:892	a prevailing viscous character	863:892	a prevailing viscous character	863:892	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	3	11	theme	main	413:416	arg1	component					418:426	the main component	409:426	the main component	409:426	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	3	11	theme	main	413:416	arg1	xylan					399:403	The SFG xylan	391:403	The SFG xylan	391:403	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	4	12	theme	extraction	625:634	arg1	temperature					636:646	the extraction temperature	621:646	the extraction temperature	621:646	The phenolic compounds were also affected by the extraction temperature, influencing the antioxidant capacity of the gum.
30879663	7	13	theme	extraction	1098:1107	arg1	temperatures					1109:1120	high extraction temperatures	1093:1120	high extraction temperatures	1093:1120	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	6	14	theme	prevailing	865:874	arg1	character					884:892	a prevailing viscous character	863:892	a prevailing viscous character	863:892	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	5	15	theme	SFG	735:737	arg1	solutions					747:755	SFG aqueous solutions	735:755	SFG aqueous solutions	735:755	For all the extraction temperatures, SFG aqueous solutions showed a shear time-independent and shear-thinning behavior.
30879663	3	16	theme	SFG	395:397	arg1	component					418:426	the main component	409:426	the main component	409:426	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	3	16	theme	SFG	395:397	arg1	xylan					399:403	The SFG xylan	391:403	The SFG xylan	391:403	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	2	17	theme	SFG	328:330	arg1	extract					332:338	SFG extract	328:338	SFG extract	328:338	The yield of SFG extract increased as the extraction temperature increased.
30879663	7	18	theme	higher	1048:1053	arg1	viscous					1055:1061	higher viscous and elastic properties	1048:1084	viscous	1055:1061	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	1	19	dep	temperatures	149:160	arg1	40					167:168	40	167:168	40	167:168	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	1	19	dep	temperatures	149:160	arg1	60 °C					175:179	60 °C	175:179	60 °C	175:179	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	0	20	theme	temperature	21:31	arg1	Effect					0:5	Effect	0:5	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.	0:97	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	4	21	theme	gum	693:695	arg1	capacity					677:684	the antioxidant capacity	661:684	the antioxidant capacity of the gum	661:695	The phenolic compounds were also affected by the extraction temperature, influencing the antioxidant capacity of the gum.
30879663	7	22	theme	extraction	1017:1026	arg1	temperatures					1028:1039	low extraction temperatures	1013:1039	low extraction temperatures	1013:1039	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	1	23	theme	Soluble	99:105	arg1	SFG					121:123	SFG	121:123	SFG	121:123	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	1	23	theme	Soluble	99:105	arg1	gum					116:118	Soluble flaxseed gum	99:118	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C)	99:180	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	5	24	theme	shear-thinning	793:806	arg1	behavior					808:815	a shear time-independent and shear-thinning behavior	764:815	a shear time-independent and shear-thinning behavior	764:815	For all the extraction temperatures, SFG aqueous solutions showed a shear time-independent and shear-thinning behavior.
30879663	0	25	theme	extraction	10:19	arg1	temperature					21:31	extraction temperature	10:31	extraction temperature	10:31	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	1	26	theme	phenolics	241:249	arg1	composition					251:261	phenolics composition	241:261	phenolics composition	241:261	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	1	27	theme	flaxseed	107:114	arg1	SFG					121:123	SFG	121:123	SFG	121:123	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	1	27	theme	flaxseed	107:114	arg1	gum					116:118	Soluble flaxseed gum	99:118	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C)	99:180	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	2	28	theme	extraction	357:366	arg1	temperature					368:378	the extraction temperature	353:378	the extraction temperature	353:378	The yield of SFG extract increased as the extraction temperature increased.
30879663	0	29	theme	rheological	36:46	arg1	behavior					48:55	rheological behavior	36:55	rheological behavior	36:55	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	4	30	theme	antioxidant	665:675	arg1	capacity					677:684	the antioxidant capacity	661:684	the antioxidant capacity of the gum	661:695	The phenolic compounds were also affected by the extraction temperature, influencing the antioxidant capacity of the gum.
30879663	5	31	theme	aqueous	739:745	arg1	solutions					747:755	SFG aqueous solutions	735:755	SFG aqueous solutions	735:755	For all the extraction temperatures, SFG aqueous solutions showed a shear time-independent and shear-thinning behavior.
30879663	6	32	theme	temperature	930:940	arg1	decrease					903:910	the decrease	899:910	the decrease of the extraction temperature	899:940	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	7	33	theme	low	1013:1015	arg1	temperatures					1028:1039	low extraction temperatures	1013:1039	low extraction temperatures	1013:1039	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	6	34	theme	oscillatory	831:841	arg1	measurements					843:854	oscillatory measurements	831:854	oscillatory measurements	831:854	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	5	35	theme	time-independent	772:787	arg1	behavior					808:815	a shear time-independent and shear-thinning behavior	764:815	a shear time-independent and shear-thinning behavior	764:815	For all the extraction temperatures, SFG aqueous solutions showed a shear time-independent and shear-thinning behavior.
30879663	7	36	theme	high	1093:1096	arg1	temperatures					1109:1120	high extraction temperatures	1093:1120	high extraction temperatures	1093:1120	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	1	37	theme	surface	264:270	arg1	charge					272:277	surface charge	264:277	surface charge	264:277	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	5	38	theme	extraction	710:719	arg1	temperatures					721:732	all the extraction temperatures	702:732	all the extraction temperatures	702:732	For all the extraction temperatures, SFG aqueous solutions showed a shear time-independent and shear-thinning behavior.
30879663	1	39	theme	rheological	291:301	arg1	properties					303:312	rheological properties	291:312	rheological properties	291:312	Soluble flaxseed gum (SFG) extracted at different temperatures (25, 40, and 60 °C) was analyzed in relation to the yield, polysaccharides and phenolics composition, surface charge, color, and rheological properties.
30879663	3	40	theme	extraction	443:452	arg1	temperature					454:464	the extraction temperature	439:464	the extraction temperature	439:464	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	3	41	from	chain	513:517	arg1	reduction					473:481	a reduction	471:481	a reduction of substituents on the xylose chain	471:517	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	3	42	theme	extraction	552:561	arg1	temperature					563:573	the extraction temperature	548:573	the extraction temperature	548:573	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	2	43	theme	extract	332:338	arg1	yield					319:323	The yield	315:323	The yield of SFG extract	315:338	The yield of SFG extract increased as the extraction temperature increased.
30879663	6	44	theme	G	981:981	arg1	increase					957:964	an increase	954:964	an increase of both G' and G"	954:982	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
30879663	0	45	theme	antioxidant	61:71	arg1	capacity					73:80	antioxidant capacity	61:80	antioxidant capacity	61:80	Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum.
30879663	7	46	theme	antioxidant	1136:1146	arg1	activity					1148:1155	the antioxidant activity	1132:1155	the antioxidant activity	1132:1155	Therefore, SFG extracted at low extraction temperatures showed higher viscous and elastic properties, while high extraction temperatures increased the antioxidant activity.
30879663	3	47	from	reduction	473:481	arg1	chain					513:517	the xylose chain	502:517	the xylose chain	502:517	The SFG xylan was the main component regardless the extraction temperature, but a reduction of substituents on the xylose chain was observed when increasing the extraction temperature.
30879663	6	48	theme	extraction	919:928	arg1	temperature					930:940	the extraction temperature	915:940	the extraction temperature	915:940	Furthermore, oscillatory measurements showed a prevailing viscous character, but the decrease of the extraction temperature resulted in an increase of both G' and G".
29734031	3	0	theme	surface	678:684	arg1	area					686:689	the surface area	674:689	the surface area	674:689	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	1	theme	electrochemical	763:777	arg1	performances					779:790	improved electrochemical performances	754:790	improved electrochemical performances	754:790	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	2	theme	CMC	650:652	arg1	CMC					650:652	CMC	650:652	CMC	650:652	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	2	theme	CMC	650:652	arg1	amount					640:645	a moderate amount	629:645	a moderate amount of CMC	629:652	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	3	theme	moderate	631:638	arg1	CMC					650:652	CMC	650:652	CMC	650:652	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	3	theme	moderate	631:638	arg1	amount					640:645	a moderate amount	629:645	a moderate amount of CMC	629:652	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	2	4	theme	composites	539:548	arg1	properties					513:522	the textural and surface chemical properties	479:522	the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure	479:612	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	1	5	theme	pHzpc	371:375	arg1	analyses					377:384	pHzpc analyses	371:384	pHzpc analyses	371:384	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	6	theme	carboxymethyl	152:164	arg1	CMC					176:178	CMC	176:178	CMC	176:178	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	6	theme	carboxymethyl	152:164	arg1	chitosan					166:173	carboxymethyl chitosan	152:173	carboxymethyl chitosan (CMC)	152:179	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	6	7	theme	sensitive	1366:1374	arg1	biosensor					1338:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	7	theme	sensitive	1366:1374	arg1	sensor					1399:1404	a sensitive, rapid, and disposable sensor	1364:1404	a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity	1364:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	8	theme	environmental	1575:1587	arg1	fields					1589:1594	the biological, clinical and environmental fields	1546:1594	the biological, clinical and environmental fields	1546:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	2	9	theme	LDH-CMC	531:537	arg1	composites					539:548	the LDH-CMC composites	527:548	the LDH-CMC composites	527:548	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	2	10	theme	2D	593:594	arg1	structure					604:612	the 2D layered structure	589:612	the 2D layered structure	589:612	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	4	11	theme	biocompatible	975:987	arg1	microenvironment					989:1004	a biocompatible microenvironment	973:1004	a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion	973:1045	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	12	theme	carbon	912:917	arg1	electrode					924:932	the glass carbon (GC) electrode	902:932	the glass carbon (GC) electrode	902:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	2	13	theme	CMC	462:464	arg1	use					455:457	The use	451:457	The use of CMC	451:464	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	5	14	theme	HRP	1078:1080	arg1	biosensor					1082:1090	The HRP biosensor	1074:1090	The HRP biosensor	1074:1090	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	6	15	theme	low	1411:1413	arg1	cost					1415:1418	low cost	1411:1418	low cost	1411:1418	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	16	theme	high	1442:1445	arg1	selectivity					1447:1457	high selectivity	1442:1457	high selectivity	1442:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	3	17	theme	improved	754:761	arg1	performances					779:790	improved electrochemical performances	754:790	improved electrochemical performances	754:790	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	5	18	with	0.02-6.0 mmol·L-1	1194:1210	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	1	19	theme	cyclic	387:392	arg1	voltammetry					394:404	cyclic voltammetry	387:404	cyclic voltammetry	387:404	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	6	20	from	detection	1519:1527	arg1	fields					1589:1594	the biological, clinical and environmental fields	1546:1594	the biological, clinical and environmental fields	1546:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	0	21	theme	H2O2	109:112	arg1	determination					92:104	the determination	88:104	the determination of H2O2	88:112	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	6	22	theme	real-time	1509:1517	arg1	detection					1519:1527	the real-time detection	1505:1527	the real-time detection of trace H2O2 in the biological, clinical and environmental fields	1505:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	4	23	theme	surface	1060:1066	arg1	area					1068:1071	the high surface area	1051:1071	the high surface area	1051:1071	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	6	24	used	used	1496:1499	arg2	biosensor					1479:1487	the proposed biosensor	1466:1487	the proposed biosensor	1466:1487	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	0	25	theme	peroxide	9:16	arg1	biosensor					18:26	Hydrogen peroxide biosensor	0:26	Hydrogen peroxide biosensor	0:26	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	1	26	theme	UV-vis	334:339	arg1	DRS					341:343	UV-vis DRS	334:343	UV-vis DRS	334:343	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	6	27	theme	H2O2	1538:1541	arg1	detection					1519:1527	the real-time detection	1505:1527	the real-time detection of trace H2O2 in the biological, clinical and environmental fields	1505:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	4	28	theme	high	1055:1058	arg1	area					1068:1071	the high surface area	1051:1071	the high surface area	1051:1071	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	6	29	theme	easy	1421:1424	arg1	preparation					1426:1436	easy preparation	1421:1436	easy preparation	1421:1436	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	0	30	theme	Hydrogen	0:7	arg1	peroxide					9:16	Hydrogen peroxide	0:16	Hydrogen peroxide biosensor	0:26	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	4	31	theme	LDH-CMC	797:803	arg1	composite					805:813	The LDH-CMC composite	793:813	The LDH-CMC composite	793:813	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	31	theme	LDH-CMC	797:803	arg1	matrix					837:842	a support matrix	827:842	a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode	827:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	6	32	theme	proposed	1325:1332	arg1	biosensor					1338:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	32	theme	proposed	1325:1332	arg1	sensor					1399:1404	a sensitive, rapid, and disposable sensor	1364:1404	a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity	1364:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	33	theme	trace	1532:1536	arg1	H2O2					1538:1541	trace H2O2	1532:1541	trace H2O2	1532:1541	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	1	34	theme	electrochemical	411:425	arg1	spectroscopy					437:448	electrochemical impedance spectroscopy	411:448	electrochemical impedance spectroscopy	411:448	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	2	35	theme	layered	596:602	arg1	structure					604:612	the 2D layered structure	589:612	the 2D layered structure	589:612	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	1	36	theme	one-step	261:268	arg1	method					275:280	the one-step urea method	257:280	the one-step urea method	257:280	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	2	37	theme	textural	483:490	arg1	properties					513:522	the textural and surface chemical properties	479:522	the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure	479:612	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	6	38	theme	HRP	1334:1336	arg1	biosensor					1338:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	38	theme	HRP	1334:1336	arg1	sensor					1399:1404	a sensitive, rapid, and disposable sensor	1364:1404	a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity	1364:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	5	39	theme	detection	1223:1231	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	40	theme	low	1219:1221	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	1	41	theme	2D	185:186	arg1	LDH					219:221	LDH	219:221	LDH	219:221	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	41	theme	2D	185:186	arg1	hydroxide					208:216	2D ZnAl layered double hydroxide	185:216	2D ZnAl layered double hydroxide (LDH)	185:222	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	0	42	theme	chitosan/2D	37:47	arg1	composite					74:82	chitosan/2D layered double hydroxide composite	37:82	chitosan/2D layered double hydroxide composite for the determination of H2O2	37:112	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	1	43	theme	urea	270:273	arg1	method					275:280	the one-step urea method	257:280	the one-step urea method	257:280	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	4	44	theme	peroxidase	882:891	arg1	immobilization					852:865	the immobilization	848:865	the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode	848:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	45	theme	H2O2	1032:1035	arg1	diffusion					1037:1045	H2O2 diffusion	1032:1045	H2O2 diffusion	1032:1045	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	1	46	theme	ZnAl	188:191	arg1	LDH					219:221	LDH	219:221	LDH	219:221	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	46	theme	ZnAl	188:191	arg1	hydroxide					208:216	2D ZnAl layered double hydroxide	185:216	2D ZnAl layered double hydroxide (LDH)	185:222	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	4	47	theme	GC	920:921	arg1	electrode					924:932	the glass carbon (GC) electrode	902:932	the glass carbon (GC) electrode	902:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	48	used	used	819:822	arg2	composite					805:813	The LDH-CMC composite	793:813	The LDH-CMC composite	793:813	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	48	used	used	819:822	arg2	matrix					837:842	a support matrix	827:842	a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode	827:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	49	theme	horseradish	870:880	arg1	HRP					894:896	HRP	894:896	HRP	894:896	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	49	theme	horseradish	870:880	arg1	peroxidase					882:891	horseradish peroxidase	870:891	horseradish peroxidase (HRP)	870:897	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	6	50	theme	clinical	1562:1569	arg1	fields					1589:1594	the biological, clinical and environmental fields	1546:1594	the biological, clinical and environmental fields	1546:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	1	51	theme	impedance	427:435	arg1	spectroscopy					437:448	electrochemical impedance spectroscopy	411:448	electrochemical impedance spectroscopy	411:448	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	52	theme	layered	193:199	arg1	LDH					219:221	LDH	219:221	LDH	219:221	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	52	theme	layered	193:199	arg1	hydroxide					208:216	2D ZnAl layered double hydroxide	185:216	2D ZnAl layered double hydroxide (LDH)	185:222	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	0	53	theme	double	57:62	arg1	composite					74:82	chitosan/2D layered double hydroxide composite	37:82	chitosan/2D layered double hydroxide composite for the determination of H2O2	37:112	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	6	54	theme	disposable	1388:1397	arg1	biosensor					1338:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	54	theme	disposable	1388:1397	arg1	sensor					1399:1404	a sensitive, rapid, and disposable sensor	1364:1404	a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity	1364:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	4	55	from	immobilization	852:865	arg1	electrode					924:932	the glass carbon (GC) electrode	902:932	the glass carbon (GC) electrode	902:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	5	56	theme	storage	1302:1308	arg1	stability					1310:1318	long-term storage stability	1292:1318	long-term storage stability	1292:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	0	57	theme	layered	49:55	arg1	composite					74:82	chitosan/2D layered double hydroxide composite	37:82	chitosan/2D layered double hydroxide composite for the determination of H2O2	37:112	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	3	58	theme	permanent	699:707	arg1	density					716:722	the permanent charge density	695:722	the permanent charge density	695:722	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	4	59	theme	support	829:835	arg1	composite					805:813	The LDH-CMC composite	793:813	The LDH-CMC composite	793:813	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	4	59	theme	support	829:835	arg1	matrix					837:842	a support matrix	827:842	a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode	827:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	6	60	theme	biological	1550:1559	arg1	fields					1589:1594	the biological, clinical and environmental fields	1546:1594	the biological, clinical and environmental fields	1546:1594	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	5	61	theme	stability	1310:1318	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	6	62	with	sensor	1399:1404	arg1	cost					1415:1418	low cost	1411:1418	low cost	1411:1418	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	62	with	sensor	1399:1404	arg1	preparation					1426:1436	easy preparation	1421:1436	easy preparation	1421:1436	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	62	with	sensor	1399:1404	arg1	selectivity					1447:1457	high selectivity	1442:1457	high selectivity	1442:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	5	63	theme	linear	1178:1183	arg1	range					1185:1189	a wide linear range	1171:1189	a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1171:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	3	64	theme	charge	709:714	arg1	density					716:722	the permanent charge density	695:722	the permanent charge density	695:722	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	2	65	theme	chemical	504:511	arg1	properties					513:522	the textural and surface chemical properties	479:522	the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure	479:612	The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure.
29734031	5	66	theme	fast	1133:1136	arg1	response					1138:1145	fast response	1133:1145	fast response	1133:1145	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	6	67	theme	proposed	1470:1477	arg1	biosensor					1479:1487	the proposed biosensor	1466:1487	the proposed biosensor	1466:1487	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	1	68	theme	double	201:206	arg1	LDH					219:221	LDH	219:221	LDH	219:221	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	1	68	theme	double	201:206	arg1	hydroxide					208:216	2D ZnAl layered double hydroxide	185:216	2D ZnAl layered double hydroxide (LDH)	185:222	The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy.
29734031	5	69	dep	sensitivity	1117:1127	arg1	a					1102:1102	a	1102:1102	a	1102:1102	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	70	theme	long-term	1292:1300	arg1	stability					1310:1318	long-term storage stability	1292:1318	long-term storage stability	1292:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	71	theme	good	1257:1260	arg1	ability					1280:1286	good anti-interference ability	1257:1286	good anti-interference ability	1257:1286	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	72	theme	12.4 μmol·L-1	1242:1254	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	4	73	theme	glass	906:910	arg1	electrode					924:932	the glass carbon (GC) electrode	902:932	the glass carbon (GC) electrode	902:932	The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area.
29734031	5	74	theme	wide	1173:1176	arg1	range					1185:1189	a wide linear range	1171:1189	a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1171:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	75	theme	anti-interference	1262:1278	arg1	ability					1280:1286	good anti-interference ability	1257:1286	good anti-interference ability	1257:1286	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	76	theme	0.02-6.0 mmol·L-1	1194:1210	arg1	range					1185:1189	a wide linear range	1171:1189	a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1171:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	77	theme	satisfactory	1104:1115	arg1	<3 s					1148:1151	<3 s	1148:1151	<3 s	1148:1151	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	5	77	theme	satisfactory	1104:1115	arg1	sensitivity					1117:1127	satisfactory sensitivity	1104:1127	satisfactory sensitivity	1104:1127	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	0	78	theme	hydroxide	64:72	arg1	composite					74:82	chitosan/2D layered double hydroxide composite	37:82	chitosan/2D layered double hydroxide composite for the determination of H2O2	37:112	Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.
29734031	5	79	theme	ability	1280:1286	arg1	limit					1233:1237	a low detection limit	1217:1237	a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability	1217:1318	The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability.
29734031	6	80	theme	rapid	1377:1381	arg1	biosensor					1338:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor	1321:1346	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	6	80	theme	rapid	1377:1381	arg1	sensor					1399:1404	a sensitive, rapid, and disposable sensor	1364:1404	a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity	1364:1457	The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.
29734031	3	81	theme	composites	731:740	arg1	density					716:722	the permanent charge density	695:722	the permanent charge density	695:722	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
29734031	3	81	theme	composites	731:740	arg1	area					686:689	the surface area	674:689	the surface area	674:689	Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances.
31759005	5	0	theme	significant	582:592	arg1	effect					594:599	no significant effect	579:599	no significant effect	579:599	0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus.
31759005	6	1	theme	DNA	646:648	arg1	concentration					650:662	DNA concentration	646:662	DNA concentration of fecal bacteria cultured with 1% AP	646:700	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	10	2	theme	health	1237:1242	arg1	function					1249:1256	the health care function	1233:1256	the health care function of apple	1233:1265	These data suggest that AP could promote the growth of B. longum indirectly, and provide another basis to understand the health care function of apple.
31759005	7	3	theme	Lactobacillus	808:820	arg1	higher					874:879	higher	874:879	higher	874:879	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	7	3	theme	Lactobacillus	808:820	arg1	number					778:783	the number	774:783	the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP	774:854	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	4	4	theme	TA	529:530	arg1	cloning					532:538	TA cloning	529:538	TA cloning	529:538	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	4	5	theme	bacteria	475:482	arg1	composition					454:464	the composition	450:464	the composition of fecal bacteria of normal SD rats	450:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	3	6	dep	longum	366:371	arg1	rhamnosus					417:425	L. rhamnosus	414:425	L. rhamnosus	414:425	In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus).
31759005	3	6	dep	longum	366:371	arg1	longum					377:382	B. longum	374:382	B. longum	374:382	In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus).
31759005	10	7	theme	care	1244:1247	arg1	function					1249:1256	the health care function	1233:1256	the health care function of apple	1233:1265	These data suggest that AP could promote the growth of B. longum indirectly, and provide another basis to understand the health care function of apple.
31759005	9	8	theme	AP	1067:1068	arg1	products					1055:1062	the metabolic products	1041:1062	the metabolic products of AP	1041:1068	AP can be utilized by one E. coli and the metabolic products of AP could enhance the proliferation of B. longum.
31759005	8	9	theme	coli	938:941	arg1	strains					915:921	Three strains	909:921	Three strains of escherichia coli (E. coli) in fecal bacteria	909:969	Three strains of escherichia coli (E. coli) in fecal bacteria were screened out and analyzed.
31759005	4	10	theme	fecal	469:473	arg1	bacteria					475:482	fecal bacteria	469:482	fecal bacteria of normal SD rats	469:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	9	11	theme	metabolic	1045:1053	arg1	products					1055:1062	the metabolic products	1041:1062	the metabolic products of AP	1041:1068	AP can be utilized by one E. coli and the metabolic products of AP could enhance the proliferation of B. longum.
31759005	7	12	theme	Bifidobacterium	788:802	arg1	higher					874:879	higher	874:879	higher	874:879	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	7	12	theme	Bifidobacterium	788:802	arg1	number					778:783	the number	774:783	the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP	774:854	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	7	13	theme	control	894:900	arg1	group					902:906	control group	894:906	control group	894:906	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	4	14	theme	AP	444:445	arg1	effects					433:439	The effects	429:439	The effects of AP on the composition of fecal bacteria of normal SD rats	429:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	8	15	dep	coli	938:941	arg1	coli					947:950	E. coli	944:950	E. coli	944:950	Three strains of escherichia coli (E. coli) in fecal bacteria were screened out and analyzed.
31759005	5	16	theme	%	567:567	arg1	AP					569:570	0.125-2% AP	560:570	0.125-2% AP	560:570	0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus.
31759005	5	17	theme	0.125-2	560:566	arg1	AP					569:570	0.125-2% AP	560:570	0.125-2% AP	560:570	0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus.
31759005	7	18	theme	%	851:851	arg1	AP					853:854	1% AP	850:854	1% AP	850:854	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	8	19	theme	fecal	956:960	arg1	bacteria					962:969	fecal bacteria	956:969	fecal bacteria	956:969	Three strains of escherichia coli (E. coli) in fecal bacteria were screened out and analyzed.
31759005	7	20	theme	1	850:850	arg1	%					851:851	%	851:851	%	851:851	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	0	21	theme	Apple	0:4	arg1	polysaccharide					6:19	Apple polysaccharide	0:19	Apple polysaccharide	0:19	Apple polysaccharide could promote the growth of Bifidobacterium longum.
31759005	3	22	dep	Lactobacillus	389:401	arg1	rhamnosus					403:411	Lactobacillus rhamnosus	389:411	Lactobacillus rhamnosus	389:411	In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus).
31759005	2	23	theme	high-fat-induced	208:223	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	4	24	from	effects	433:439	arg1	composition					454:464	the composition	450:464	the composition of fecal bacteria of normal SD rats	450:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	6	25	theme	%	697:697	arg1	AP					699:700	1% AP	696:700	1% AP	696:700	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	10	26	theme	longum	1174:1179	arg1	growth					1161:1166	the growth	1157:1166	the growth of B. longum	1157:1179	These data suggest that AP could promote the growth of B. longum indirectly, and provide another basis to understand the health care function of apple.
31759005	1	27	theme	regulating	100:109	arg1	microbiome					111:120	regulating microbiome	100:120	regulating microbiome	100:120	It is widely accepted that regulating microbiome could improve human health.
31759005	10	28	theme	apple	1261:1265	arg1	function					1249:1256	the health care function	1233:1256	the health care function of apple	1233:1265	These data suggest that AP could promote the growth of B. longum indirectly, and provide another basis to understand the health care function of apple.
31759005	5	29	theme	rhamnosus	635:643	arg1	growth					608:613	the growth	604:613	the growth of B. longum and L. rhamnosus	604:643	0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus.
31759005	2	30	theme	polysaccharide	179:192	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	4	31	theme	normal	487:492	arg1	rats					497:500	normal SD rats	487:500	normal SD rats	487:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	2	32	theme	apple	173:177	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	6	33	theme	1	696:696	arg1	%					697:697	%	697:697	%	697:697	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	9	34	theme	longum	1108:1113	arg1	proliferation					1088:1100	the proliferation	1084:1100	the proliferation of B. longum	1084:1113	AP can be utilized by one E. coli and the metabolic products of AP could enhance the proliferation of B. longum.
31759005	4	35	theme	rats	497:500	arg1	bacteria					475:482	fecal bacteria	469:482	fecal bacteria of normal SD rats	469:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	3	36	from	function	309:316	arg1	vitro					327:331	vitro	327:331	vitro	327:331	In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus).
31759005	2	37	theme	microbial	225:233	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	4	38	theme	SD	494:495	arg1	rats					497:500	normal SD rats	487:500	normal SD rats	487:500	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	2	39	theme	reversed	199:206	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	6	40	theme	bacteria	673:680	arg1	concentration					650:662	DNA concentration	646:662	DNA concentration of fecal bacteria cultured with 1% AP	646:700	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	5	41	theme	longum	621:626	arg1	growth					608:613	the growth	604:613	the growth of B. longum and L. rhamnosus	604:643	0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus.
31759005	9	42	used	utilized	1013:1020	arg2	AP					1003:1004	AP	1003:1004	AP	1003:1004	AP can be utilized by one E. coli and the metabolic products of AP could enhance the proliferation of B. longum.
31759005	6	43	theme	fecal	667:671	arg1	bacteria					673:680	fecal bacteria	667:680	fecal bacteria cultured with 1% AP	667:700	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	1	44	theme	human	136:140	arg1	health					142:147	human health	136:147	human health	136:147	It is widely accepted that regulating microbiome could improve human health.
31759005	4	45	theme	16S	544:546	arg1	sequencing					548:557	16S sequencing	544:557	16S sequencing	544:557	The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing.
31759005	3	46	theme	AP	321:322	arg1	function					309:316	the function	305:316	the function of AP in vitro	305:331	In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus).
31759005	2	47	theme	AP	195:196	arg1	dysbiosis					235:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis	173:243	We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated.
31759005	7	48	theme	fecal	825:829	arg1	flora					831:835	fecal flora	825:835	fecal flora incubated by 1% AP	825:854	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	6	49	theme	control	740:746	arg1	group					748:752	control group	740:752	control group	740:752	DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group.
31759005	0	50	theme	longum	65:70	arg1	growth					39:44	the growth	35:44	the growth of Bifidobacterium longum	35:70	Apple polysaccharide could promote the growth of Bifidobacterium longum.
31759005	7	51	from	number	778:783	arg1	flora					831:835	fecal flora	825:835	fecal flora incubated by 1% AP	825:854	qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group.
31759005	8	52	from	strains	915:921	arg1	bacteria					962:969	fecal bacteria	956:969	fecal bacteria	956:969	Three strains of escherichia coli (E. coli) in fecal bacteria were screened out and analyzed.
30611503	0	0	theme	major	72:76	arg1	factor					90:95	a major influencing factor	70:95	a major influencing factor on the texture of lotus rhizomes after cooking	70:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	0	0	theme	major	72:76	arg1	ratio					4:8	The ratio	0:8	The ratio of chelate-soluble fraction to alcohol insoluble residue	0:65	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	4	1	theme	gas	810:812	arg1	chromatography					814:827	gas chromatography	810:827	gas chromatography	810:827	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	2	theme	present	645:651	arg1	polysaccharides					629:643	The cell wall polysaccharides	615:643	The cell wall polysaccharides present in alcohol insoluble residue (AIR)	615:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	6	3	theme	component	949:957	arg1	analysis					959:966	Principal component analysis	939:966	Principal component analysis	939:966	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	6	4	theme	Principal	939:947	arg1	analysis					959:966	Principal component analysis	939:966	Principal component analysis	939:966	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	0	5	theme	influencing	78:88	arg1	factor					90:95	a major influencing factor	70:95	a major influencing factor on the texture of lotus rhizomes after cooking	70:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	0	5	theme	influencing	78:88	arg1	ratio					4:8	The ratio	0:8	The ratio of chelate-soluble fraction to alcohol insoluble residue	0:65	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	2	6	theme	lotus	464:468	arg1	rhizomes					470:477	lotus rhizomes	464:477	lotus rhizomes after cooking	464:491	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	4	7	theme	alcohol	656:662	arg1	AIR					683:685	AIR	683:685	AIR	683:685	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	7	theme	alcohol	656:662	arg1	residue					674:680	alcohol insoluble residue	656:680	alcohol insoluble residue (AIR)	656:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	8	theme	different	717:725	arg1	fractions					727:735	different fractions	717:735	different fractions	717:735	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	5	9	theme	sugar	853:857	arg1	ratios					859:864	the sugar ratios	849:864	the sugar ratios	849:864	We then calculated the sugar ratios to examine the discrepancies in molecular structure among the fractions.
30611503	6	10	theme	rhizomes	1106:1113	arg1	texture					1089:1095	the texture	1085:1095	the texture of lotus rhizomes after cooking	1085:1127	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	2	11	theme	China	363:367	arg1	regions					352:358	different regions	342:358	different regions of China	342:367	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	2	12	theme	key	425:427	arg1	factors					429:435	the key factors	421:435	the key factors that affect the texture of lotus rhizomes after cooking	421:491	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	4	13	attach	present	645:651	arg1	residue					674:680	alcohol insoluble residue	656:680	alcohol insoluble residue (AIR)	656:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	13	attach	present	645:651	arg1	AIR					683:685	AIR	683:685	AIR	683:685	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	13	attach	present	645:651	arg2	polysaccharides					629:643	The cell wall polysaccharides	615:643	The cell wall polysaccharides present in alcohol insoluble residue (AIR)	615:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	6	14	theme	lotus	1100:1104	arg1	rhizomes					1106:1113	lotus rhizomes	1100:1113	lotus rhizomes	1100:1113	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	1	15	theme	cell	164:167	arg1	polysaccharides					174:188	cell wall polysaccharides	164:188	cell wall polysaccharides	164:188	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	1	16	theme	plant-based	253:263	arg1	food					265:268	plant-based food	253:268	plant-based food	253:268	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	1	17	theme	wall	169:172	arg1	polysaccharides					174:188	cell wall polysaccharides	164:188	cell wall polysaccharides	164:188	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	0	18	theme	chelate-soluble	13:27	arg1	fraction					29:36	chelate-soluble fraction	13:36	chelate-soluble fraction	13:36	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	1	19	theme	food	265:268	arg1	texture					242:248	the texture	238:248	the texture of plant-based food after cooking	238:282	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	4	20	theme	monosaccharides	761:775	arg1	composition					746:756	the composition	742:756	the composition of monosaccharides in each fraction	742:792	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	3	21	theme	time	588:591	arg1	periods					593:599	different time periods	578:599	different time periods	578:599	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	5	22	theme	molecular	898:906	arg1	structure					908:916	molecular structure	898:916	molecular structure	898:916	We then calculated the sugar ratios to examine the discrepancies in molecular structure among the fractions.
30611503	4	23	theme	wall	624:627	arg1	polysaccharides					629:643	The cell wall polysaccharides	615:643	The cell wall polysaccharides present in alcohol insoluble residue (AIR)	615:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	0	24	theme	rhizomes	121:128	arg1	texture					104:110	the texture	100:110	the texture of lotus rhizomes after cooking	100:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	6	25	theme	chelate-soluble	1017:1031	arg1	fraction					1033:1040	chelate-soluble fraction	1017:1040	chelate-soluble fraction (CSF)	1017:1046	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	6	25	theme	chelate-soluble	1017:1031	arg1	CSF					1043:1045	CSF	1043:1045	CSF	1043:1045	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	0	26	theme	fraction	29:36	arg1	factor					90:95	a major influencing factor	70:95	a major influencing factor on the texture of lotus rhizomes after cooking	70:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	0	26	theme	fraction	29:36	arg1	ratio					4:8	The ratio	0:8	The ratio of chelate-soluble fraction to alcohol insoluble residue	0:65	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	0	27	theme	lotus	115:119	arg1	rhizomes					121:128	lotus rhizomes	115:128	lotus rhizomes after cooking	115:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	6	28	theme	regression	972:981	arg1	analysis					983:990	regression analysis	972:990	regression analysis	972:990	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	6	29	theme	fraction	1033:1040	arg1	ratio					1008:1012	the ratio	1004:1012	the ratio of chelate-soluble fraction (CSF) to AIR	1004:1053	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	6	29	theme	fraction	1033:1040	arg1	factor					1068:1073	the major factor	1058:1073	the major factor affecting the texture of lotus rhizomes after cooking	1058:1127	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	0	30	theme	insoluble	49:57	arg1	residue					59:65	alcohol insoluble residue	41:65	alcohol insoluble residue	41:65	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	3	31	theme	different	578:586	arg1	periods					593:599	different time periods	578:599	different time periods	578:599	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	3	32	theme	fresh	520:524	arg1	samples					526:532	fresh samples	520:532	fresh samples	520:532	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	2	33	theme	rhizomes	313:320	arg1	varieties					294:302	18 varieties	291:302	18 varieties of lotus rhizomes	291:320	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	2	33	theme	rhizomes	313:320	arg1	rhizomes					313:320	lotus rhizomes	307:320	lotus rhizomes	307:320	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	0	34	theme	alcohol	41:47	arg1	residue					59:65	alcohol insoluble residue	41:65	alcohol insoluble residue	41:65	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	1	35	from	factor	228:233	arg1	texture					242:248	the texture	238:248	the texture of plant-based food after cooking	238:282	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	3	36	theme	samples	526:532	arg1	hardness					507:514	hardness	507:514	hardness	507:514	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	3	36	theme	samples	526:532	arg1	texture					498:504	The texture	494:504	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods	494:599	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	2	37	theme	lotus	307:311	arg1	rhizomes					313:320	lotus rhizomes	307:320	lotus rhizomes	307:320	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	4	38	from	residue	674:680	arg1	present					645:651	present	645:651	present	645:651	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	3	39	theme	thermal	556:562	arg1	treatment					564:572	thermal treatment	556:572	thermal treatment for different time periods	556:599	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	4	40	from	composition	746:756	arg1	fraction					785:792	each fraction	780:792	each fraction	780:792	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	6	41	theme	major	1062:1066	arg1	ratio					1008:1012	the ratio	1004:1012	the ratio of chelate-soluble fraction (CSF) to AIR	1004:1053	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	6	41	theme	major	1062:1066	arg1	factor					1068:1073	the major factor	1058:1073	the major factor affecting the texture of lotus rhizomes after cooking	1058:1127	Principal component analysis and regression analysis showed that the ratio of chelate-soluble fraction (CSF) to AIR is the major factor affecting the texture of lotus rhizomes after cooking.
30611503	0	42	from	factor	90:95	arg1	texture					104:110	the texture	100:110	the texture of lotus rhizomes after cooking	100:142	The ratio of chelate-soluble fraction to alcohol insoluble residue is a major influencing factor on the texture of lotus rhizomes after cooking.
30611503	4	43	theme	insoluble	664:672	arg1	AIR					683:685	AIR	683:685	AIR	683:685	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	43	theme	insoluble	664:672	arg1	residue					674:680	alcohol insoluble residue	656:680	alcohol insoluble residue (AIR)	656:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	1	44	theme	major	210:214	arg1	differences					149:159	The differences	145:159	The differences in cell wall polysaccharides	145:188	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	1	44	theme	major	210:214	arg1	factor					228:233	a major influencing factor	208:233	a major influencing factor on the texture of plant-based food after cooking	208:282	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	3	45	theme	samples	542:548	arg1	hardness					507:514	hardness	507:514	hardness	507:514	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	3	45	theme	samples	542:548	arg1	texture					498:504	The texture	494:504	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods	494:599	The texture (hardness) of fresh samples and the samples after thermal treatment for different time periods was examined.
30611503	2	46	theme	different	342:350	arg1	regions					352:358	different regions	342:358	different regions of China	342:367	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
30611503	5	47	from	discrepancies	881:893	arg1	structure					908:916	molecular structure	898:916	molecular structure	898:916	We then calculated the sugar ratios to examine the discrepancies in molecular structure among the fractions.
30611503	1	48	theme	influencing	216:226	arg1	differences					149:159	The differences	145:159	The differences in cell wall polysaccharides	145:188	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	1	48	theme	influencing	216:226	arg1	factor					228:233	a major influencing factor	208:233	a major influencing factor on the texture of plant-based food after cooking	208:282	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	1	49	from	differences	149:159	arg1	polysaccharides					174:188	cell wall polysaccharides	164:188	cell wall polysaccharides	164:188	The differences in cell wall polysaccharides are considered as a major influencing factor on the texture of plant-based food after cooking.
30611503	4	50	theme	cell	619:622	arg1	polysaccharides					629:643	The cell wall polysaccharides	615:643	The cell wall polysaccharides present in alcohol insoluble residue (AIR)	615:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	51	from	present	645:651	arg1	AIR					683:685	AIR	683:685	AIR	683:685	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	4	51	from	present	645:651	arg1	residue					674:680	alcohol insoluble residue	656:680	alcohol insoluble residue (AIR)	656:686	The cell wall polysaccharides present in alcohol insoluble residue (AIR) were further subdivided into different fractions, and the composition of monosaccharides in each fraction was analyzed by gas chromatography.
30611503	2	52	theme	rhizomes	470:477	arg1	texture					453:459	the texture	449:459	the texture of lotus rhizomes after cooking	449:491	Here, 18 varieties of lotus rhizomes were collected from different regions of China and subjected to analysis, with the aim to identify the key factors that affect the texture of lotus rhizomes after cooking.
31394182	0	0	link	crosslinked	81:91	arg1	derivative					100:109	a crosslinked starch derivative	79:109	a crosslinked starch derivative	79:109	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	4	1	theme	physical	679:686	arg1	blend					688:692	a drug-polymer physical blend	664:692	a drug-polymer physical blend	664:692	Two deterrent compositions were prepared, a drug-polymer physical blend and a drug-polymer chemical complex.
31394182	6	2	theme	major	981:985	arg1	role					987:990	a major role	979:990	a major role	979:990	The studies revealed that the complexation with the drug played a major role in total drug entrapment.
31394182	5	3	theme	complexed	754:762	arg1	form					764:767	its complexed form	750:767	its complexed form	750:767	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	3	4	from	swells	488:493	arg1	and					498:500	and	498:500	and	498:500	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	4	5	theme	drug-polymer	666:677	arg1	blend					688:692	a drug-polymer physical blend	664:692	a drug-polymer physical blend	664:692	Two deterrent compositions were prepared, a drug-polymer physical blend and a drug-polymer chemical complex.
31394182	9	6	from	release	1347:1353	arg1	media					1385:1389	the gastric and intestinal media	1358:1389	the gastric and intestinal media	1358:1389	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	0	7	theme	starch	93:98	arg1	derivative					100:109	a crosslinked starch derivative	79:109	a crosslinked starch derivative	79:109	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	7	8	theme	immediate	1087:1095	arg1	release					1102:1108	a complete and immediate drug release	1072:1108	a complete and immediate drug release	1072:1108	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	8	9	theme	simulated	1189:1197	arg1	media					1210:1214	the simulated intestinal media	1185:1214	the simulated intestinal media	1185:1214	Moreover, the dissolution studies in the simulated intestinal media ruled out the re-complexation potential between the drug and the polymer.
31394182	2	10	theme	sodium	282:287	arg1	glycolate					296:304	a crosslinked sodium starch glycolate	268:304	a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction	268:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	2	10	theme	sodium	282:287	arg1	X-SSG					307:311	X-SSG	307:311	X-SSG	307:311	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	3	11	theme	solution	532:539	arg1	portion					512:518	a portion	510:518	a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection	510:619	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	9	12	theme	legitimate	1401:1410	arg1	use					1412:1414	the legitimate use	1397:1414	the legitimate use	1397:1414	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	0	13	theme	crosslinked	81:91	arg1	derivative					100:109	a crosslinked starch derivative	79:109	a crosslinked starch derivative	79:109	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	7	14	theme	drug	1097:1100	arg1	release					1102:1108	a complete and immediate drug release	1072:1108	a complete and immediate drug release	1072:1108	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	2	15	theme	crosslinked	270:280	arg1	glycolate					296:304	a crosslinked sodium starch glycolate	268:304	a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction	268:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	2	15	theme	crosslinked	270:280	arg1	X-SSG					307:311	X-SSG	307:311	X-SSG	307:311	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	7	16	theme	complete	1074:1081	arg1	release					1102:1108	a complete and immediate drug release	1072:1108	a complete and immediate drug release	1072:1108	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	8	17	theme	re-complexation	1230:1244	arg1	potential					1246:1254	the re-complexation potential	1226:1254	the re-complexation potential between the drug and the polymer	1226:1287	Moreover, the dissolution studies in the simulated intestinal media ruled out the re-complexation potential between the drug and the polymer.
31394182	2	18	theme	aqueous	366:372	arg1	solutions					374:382	aqueous solutions	366:382	aqueous solutions	366:382	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	6	19	with	complexation	945:956	arg1	drug					967:970	the drug	963:970	the drug	963:970	The studies revealed that the complexation with the drug played a major role in total drug entrapment.
31394182	7	20	theme	0.1 N	1113:1117	arg1	HCl					1119:1121	0.1 N HCl	1113:1121	0.1 N HCl	1113:1121	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	9	21	theme	proposed	1294:1301	arg1	composition					1309:1319	The proposed X-SSG composition	1290:1319	The proposed X-SSG composition	1290:1319	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	6	22	theme	total	995:999	arg1	entrapment					1006:1015	total drug entrapment	995:1015	total drug entrapment	995:1015	The studies revealed that the complexation with the drug played a major role in total drug entrapment.
31394182	4	23	theme	deterrent	626:634	arg1	compositions					636:647	Two deterrent compositions	622:647	Two deterrent compositions	622:647	Two deterrent compositions were prepared, a drug-polymer physical blend and a drug-polymer chemical complex.
31394182	2	24	theme	available	425:433	arg1	drug					420:423	the free drug	411:423	the free drug available for extraction	411:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	9	25	theme	X-SSG	1303:1307	arg1	composition					1309:1319	The proposed X-SSG composition	1290:1319	The proposed X-SSG composition	1290:1319	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	2	26	from	complex	335:341	arg1	solutions					374:382	aqueous solutions	366:382	aqueous solutions	366:382	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	1	27	theme	dangerous	186:194	arg1	route					196:200	the most dangerous route	177:200	the most dangerous route of drug abuse, the intravenous route	177:237	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	2	28	theme	drug	420:423	arg1	drug					420:423	the free drug	411:423	the free drug available for extraction	411:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	2	28	theme	drug	420:423	arg1	amount					401:406	the amount	397:406	the amount of the free drug available for extraction	397:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	4	29	theme	chemical	713:720	arg1	complex					722:728	a drug-polymer chemical complex	698:728	a drug-polymer chemical complex	698:728	Two deterrent compositions were prepared, a drug-polymer physical blend and a drug-polymer chemical complex.
31394182	9	30	theme	intravenous	1435:1445	arg1	abuse					1447:1451	an intravenous abuse	1432:1451	an intravenous abuse	1432:1451	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	2	31	theme	free	415:418	arg1	drug					420:423	the free drug	411:423	the free drug available for extraction	411:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	7	32	theme	drug-polymer	1044:1055	arg1	complex					1057:1063	the drug-polymer complex	1040:1063	the drug-polymer complex	1040:1063	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	3	33	theme	drug	527:530	arg1	solution					532:539	the drug solution	523:539	the drug solution	523:539	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	0	34	theme	in	16:17	arg1	evaluation					25:34	in vitro evaluation	16:34	in vitro evaluation	16:34	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	5	35	with	solvents	838:845	arg1	moieties					858:865	ionic moieties	852:865	ionic moieties	852:865	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	2	36	theme	starch	289:294	arg1	glycolate					296:304	a crosslinked sodium starch glycolate	268:304	a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction	268:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	2	36	theme	starch	289:294	arg1	X-SSG					307:311	X-SSG	307:311	X-SSG	307:311	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	1	37	theme	drug	205:208	arg1	route					233:237	the intravenous route	217:237	the intravenous route	217:237	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	1	37	theme	drug	205:208	arg1	abuse					210:214	drug abuse	205:214	drug abuse	205:214	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	2	38	link	crosslinked	270:280	arg1	glycolate					296:304	a crosslinked sodium starch glycolate	268:304	a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction	268:448	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	2	38	link	crosslinked	270:280	arg1	X-SSG					307:311	X-SSG	307:311	X-SSG	307:311	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	8	39	theme	dissolution	1162:1172	arg1	studies					1174:1180	the dissolution studies	1158:1180	the dissolution studies in the simulated intestinal media	1158:1214	Moreover, the dissolution studies in the simulated intestinal media ruled out the re-complexation potential between the drug and the polymer.
31394182	1	40	theme	abuse	210:214	arg1	route					196:200	the most dangerous route	177:200	the most dangerous route of drug abuse, the intravenous route	177:237	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	9	41	theme	drug	1342:1345	arg1	release					1347:1353	a desirable drug release	1330:1353	a desirable drug release in the gastric and intestinal media	1330:1389	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	3	42	theme	subsequent	600:609	arg1	injection					611:619	subsequent injection	600:619	subsequent injection	600:619	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	3	43	theme	available	565:573	arg1	volume					575:580	the available volume	561:580	the available volume for syringing and subsequent injection	561:619	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	0	44	theme	abuse-deterrent	42:56	arg1	formulation					58:68	an abuse-deterrent formulation	39:68	an abuse-deterrent formulation	39:68	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	3	45	theme	crosslinked	468:478	arg1	swells					488:493	the crosslinked polymer swells	464:493	the crosslinked polymer swells in and	464:500	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	5	46	theme	deterrence	784:793	arg1	capacity					795:802	greater deterrence capacity	776:802	greater deterrence capacity	776:802	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	1	47	theme	innovative	115:124	arg1	composition					142:152	An innovative abuse-deterrent composition	112:152	An innovative abuse-deterrent composition	112:152	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	4	48	theme	drug-polymer	700:711	arg1	complex					722:728	a drug-polymer chemical complex	698:728	a drug-polymer chemical complex	698:728	Two deterrent compositions were prepared, a drug-polymer physical blend and a drug-polymer chemical complex.
31394182	7	49	theme	first	1134:1138	arg1	15 min					1140:1145	the first 15 min	1130:1145	the first 15 min	1130:1145	Tablets prepared from the drug-polymer complex showed a complete and immediate drug release in 0.1 N HCl within the first 15 min.
31394182	3	50	link	crosslinked	468:478	arg1	swells					488:493	the crosslinked polymer swells	464:493	the crosslinked polymer swells in and	464:500	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
31394182	2	51	with	complex	335:341	arg1	drugs					357:361	cationic drugs	348:361	cationic drugs	348:361	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	9	52	theme	desirable	1332:1340	arg1	release					1347:1353	a desirable drug release	1330:1353	a desirable drug release in the gastric and intestinal media	1330:1389	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	1	53	theme	abuse-deterrent	126:140	arg1	composition					142:152	An innovative abuse-deterrent composition	112:152	An innovative abuse-deterrent composition	112:152	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	9	54	theme	gastric	1362:1368	arg1	media					1385:1389	the gastric and intestinal media	1358:1389	the gastric and intestinal media	1358:1389	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	6	55	theme	drug	1001:1004	arg1	entrapment					1006:1015	total drug entrapment	995:1015	total drug entrapment	995:1015	The studies revealed that the complexation with the drug played a major role in total drug entrapment.
31394182	5	56	from	composition	735:745	arg1	form					764:767	its complexed form	750:767	its complexed form	750:767	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	0	57	dep	in	16:17	arg1	vitro					19:23	vitro	19:23	vitro	19:23	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	8	58	theme	intestinal	1199:1208	arg1	media					1210:1214	the simulated intestinal media	1185:1214	the simulated intestinal media	1185:1214	Moreover, the dissolution studies in the simulated intestinal media ruled out the re-complexation potential between the drug and the polymer.
31394182	1	59	theme	intravenous	221:231	arg1	route					233:237	the intravenous route	217:237	the intravenous route	217:237	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	1	59	theme	intravenous	221:231	arg1	abuse					210:214	drug abuse	205:214	drug abuse	205:214	An innovative abuse-deterrent composition was developed to deter the most dangerous route of drug abuse, the intravenous route.
31394182	5	60	theme	greater	776:782	arg1	capacity					795:802	greater deterrence capacity	776:802	greater deterrence capacity	776:802	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	5	61	theme	ionic	852:856	arg1	moieties					858:865	ionic moieties	852:865	ionic moieties	852:865	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	9	62	theme	intestinal	1374:1383	arg1	media					1385:1389	the gastric and intestinal media	1358:1389	the gastric and intestinal media	1358:1389	The proposed X-SSG composition provides a desirable drug release in the gastric and intestinal media under the legitimate use while deterring an intravenous abuse.
31394182	0	63	theme	formulation	58:68	arg1	Development					0:10	Development	0:10	Development	0:10	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	0	63	theme	formulation	58:68	arg1	evaluation					25:34	in vitro evaluation	16:34	in vitro evaluation	16:34	Development and in vitro evaluation of an abuse-deterrent formulation based on a crosslinked starch derivative.
31394182	8	64	from	studies	1174:1180	arg1	media					1210:1214	the simulated intestinal media	1185:1214	the simulated intestinal media	1185:1214	Moreover, the dissolution studies in the simulated intestinal media ruled out the re-complexation potential between the drug and the polymer.
31394182	5	65	theme	physical	813:820	arg1	blend					822:826	the physical blend	809:826	the physical blend	809:826	The composition in its complexed form showed greater deterrence capacity than the physical blend except in solvents with ionic moieties, where the deterrence remained almost the same.
31394182	2	66	theme	cationic	348:355	arg1	drugs					357:361	cationic drugs	348:361	cationic drugs	348:361	The composition is based on a crosslinked sodium starch glycolate (X-SSG) that can effectively complex with cationic drugs in aqueous solutions and minimize the amount of the free drug available for extraction.
31394182	3	67	theme	polymer	480:486	arg1	swells					488:493	the crosslinked polymer swells	464:493	the crosslinked polymer swells in and	464:500	Furthermore, the crosslinked polymer swells in and entraps a portion of the drug solution by which it reduces the available volume for syringing and subsequent injection.
29337104	1	0	contain	contain	133:139	arg2	source					152:157	a valuable source	141:157	a valuable source of functional bioactive polysaccharide	141:196	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	1	0	contain	contain	133:139	arg1	seaweeds					124:131	Marine seaweeds	117:131	Marine seaweeds	117:131	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	3	1	dep	spicifera	366:374	arg1	contain					376:382	contain	376:382	contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid	376:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	7	2	theme	sulfated	809:816	arg1	polysaccharide					818:831	the purified sulfated polysaccharide	796:831	the purified sulfated polysaccharide (fraction F2)	796:845	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	7	2	theme	sulfated	809:816	arg1	F2					843:844	fraction F2	834:844	fraction F2	834:844	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	8	3	from	effects	1069:1075	arg1	lines					1090:1094	A549 cell lines	1080:1094	A549 cell lines	1080:1094	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	6	4	theme	%	758:758	arg1	activity					742:749	reducing power activity	727:749	reducing power activity	727:749	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	6	4	theme	%	758:758	arg1	150 μg/mL					713:721	150 μg/mL	713:721	150 μg/mL	713:721	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	0	5	from	potential	10:18	arg1	spicifera					90:98	spicifera	90:98	spicifera	90:98	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	5	from	potential	10:18	arg1	Vahl					101:104	Vahl	101:104	Vahl	101:104	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	7	6	dep	in	988:989	arg1	vitro					991:995	vitro	991:995	vitro	991:995	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	6	7	theme	free	667:670	arg1	radical					672:678	DPPH free radical	662:678	DPPH free radical scavenging activity of 55.55%	662:708	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	1	8	theme	functional	162:171	arg1	polysaccharide					183:196	functional bioactive polysaccharide	162:196	functional bioactive polysaccharide	162:196	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	7	9	theme	potential	930:938	arg1	activity					951:958	their potential anticancer activity	924:958	their potential anticancer activity of in A549 cell lines under in vitro condition	924:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	6	10	theme	DPPH	662:665	arg1	radical					672:678	DPPH free radical	662:678	DPPH free radical scavenging activity of 55.55%	662:708	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	3	11	from	spicifera	366:374	arg1	SPs					354:356	The isolated SPs	341:356	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid	341:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	7	12	theme	anticancer	940:949	arg1	activity					951:958	their potential anticancer activity	924:958	their potential anticancer activity of in A549 cell lines under in vitro condition	924:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	7	13	theme	present	781:787	arg1	study					789:793	the present study	777:793	the present study	777:793	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	0	14	dep	Borgeson	107:114	arg1	potential					10:18	Bioactive potential	0:18	Bioactive potential	0:18	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	14	dep	Borgeson	107:114	arg1	analysis					36:43	composition analysis	24:43	composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl)	24:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	8	15	theme	apoptotic	1059:1067	arg1	effects					1069:1075	potential apoptotic effects	1049:1075	potential apoptotic effects on A549 cell lines	1049:1094	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	3	16	theme	isolated	345:352	arg1	SPs					354:356	The isolated SPs	341:356	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid	341:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	17	theme	uranic	448:453	arg1	acid					455:458	total uranic acid	442:458	total uranic acid	442:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	1	18	theme	bioactive	173:181	arg1	polysaccharide					183:196	functional bioactive polysaccharide	162:196	functional bioactive polysaccharide	162:196	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	3	19	theme	total	393:397	arg1	sugar					399:403	total sugar	393:403	total sugar	393:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	6	20	theme	reducing	727:734	arg1	activity					742:749	reducing power activity	727:749	reducing power activity	727:749	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	7	21	theme	cell	971:974	arg1	lines					976:980	A549 cell lines	966:980	A549 cell lines under in vitro condition	966:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	2	22	theme	SPs	282:284	arg1	feature					271:277	The structural feature	256:277	The structural feature of SPs	256:284	The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis.
29337104	1	23	theme	polysaccharide	183:196	arg1	source					152:157	a valuable source	141:157	a valuable source of functional bioactive polysaccharide	141:196	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	0	24	from	analysis	36:43	arg1	spicifera					90:98	spicifera	90:98	spicifera	90:98	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	24	from	analysis	36:43	arg1	Vahl					101:104	Vahl	101:104	Vahl	101:104	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	2	25	theme	NMR	319:321	arg1	spectra					323:329	1H NMR spectra	316:329	1H NMR spectra	316:329	The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis.
29337104	6	26	theme	power	736:740	arg1	activity					742:749	reducing power activity	727:749	reducing power activity	727:749	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	0	27	theme	Bioactive	0:8	arg1	potential					10:18	Bioactive potential	0:18	Bioactive potential	0:18	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	8	28	theme	potential	1049:1057	arg1	effects					1069:1075	potential apoptotic effects	1049:1075	potential apoptotic effects on A549 cell lines	1049:1094	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	2	29	theme	1H	316:317	arg1	spectra					323:329	1H NMR spectra	316:329	1H NMR spectra	316:329	The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis.
29337104	8	30	theme	cell	1085:1088	arg1	lines					1090:1094	A549 cell lines	1080:1094	A549 cell lines	1080:1094	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	0	31	theme	composition	24:34	arg1	analysis					36:43	composition analysis	24:43	composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl)	24:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	7	32	theme	red	894:896	arg1	seaweed					898:904	red seaweed	894:904	red seaweed	894:904	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	7	33	from	activity	951:958	arg1	lines					976:980	A549 cell lines	966:980	A549 cell lines under in vitro condition	966:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	4	34	theme	SP	514:515	arg1	420 kDa					533:539	420 kDa	533:539	420 kDa	533:539	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	4	34	theme	SP	514:515	arg1	weight					504:509	The active F2 fraction molecular weight	471:509	The active F2 fraction molecular weight of SP	471:515	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	7	35	theme	in	988:989	arg1	condition					997:1005	in vitro condition	988:1005	in vitro condition	988:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	1	36	theme	main	211:214	arg1	role					216:219	main role	211:219	main role for effective anticancer activity	211:253	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	3	37	theme	sugar	399:403	arg1	%					388:388	63.3%	384:388	63.3% of total sugar	384:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	37	theme	sugar	399:403	arg1	%					410:410	21.9%	406:410	21.9% of total sulfate	406:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	37	theme	sugar	399:403	arg1	acid					455:458	total uranic acid	442:458	total uranic acid	442:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	37	theme	sugar	399:403	arg1	sugar					399:403	total sugar	393:403	total sugar	393:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	37	theme	sugar	399:403	arg1	sulfate					421:427	total sulfate	415:427	total sulfate	415:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	37	theme	sugar	399:403	arg1	%					437:437	12.6%	433:437	12.6% of total uranic acid	433:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	%					388:388	63.3%	384:388	63.3% of total sugar	384:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	%					410:410	21.9%	406:410	21.9% of total sulfate	406:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	acid					455:458	total uranic acid	442:458	total uranic acid	442:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	sugar					399:403	total sugar	393:403	total sugar	393:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	sulfate					421:427	total sulfate	415:427	total sulfate	415:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	38	theme	acid	455:458	arg1	%					437:437	12.6%	433:437	12.6% of total uranic acid	433:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	0	39	from	spicifera	90:98	arg1	potential					10:18	Bioactive potential	0:18	Bioactive potential	0:18	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	39	from	spicifera	90:98	arg1	analysis					36:43	composition analysis	24:43	composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl)	24:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	39	from	spicifera	90:98	arg1	polysaccharide					57:70	sulfated polysaccharide	48:70	sulfated polysaccharide from Acanthophora spicifera (Vahl)	48:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	3	40	theme	sulfate	421:427	arg1	%					388:388	63.3%	384:388	63.3% of total sugar	384:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	40	theme	sulfate	421:427	arg1	%					410:410	21.9%	406:410	21.9% of total sulfate	406:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	40	theme	sulfate	421:427	arg1	acid					455:458	total uranic acid	442:458	total uranic acid	442:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	40	theme	sulfate	421:427	arg1	sugar					399:403	total sugar	393:403	total sugar	393:403	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	40	theme	sulfate	421:427	arg1	sulfate					421:427	total sulfate	415:427	total sulfate	415:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	3	40	theme	sulfate	421:427	arg1	%					437:437	12.6%	433:437	12.6% of total uranic acid	433:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	8	41	theme	A549	1080:1083	arg1	lines					1090:1094	A549 cell lines	1080:1094	A549 cell lines	1080:1094	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	4	42	theme	F2	482:483	arg1	420 kDa					533:539	420 kDa	533:539	420 kDa	533:539	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	4	42	theme	F2	482:483	arg1	weight					504:509	The active F2 fraction molecular weight	471:509	The active F2 fraction molecular weight of SP	471:515	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	6	43	theme	%	708:708	arg1	activity					691:698	DPPH free radical scavenging activity	662:698	DPPH free radical scavenging activity of 55.55%	662:708	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	0	44	theme	polysaccharide	57:70	arg1	potential					10:18	Bioactive potential	0:18	Bioactive potential	0:18	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	0	44	theme	polysaccharide	57:70	arg1	analysis					36:43	composition analysis	24:43	composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl)	24:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	4	45	theme	active	475:480	arg1	420 kDa					533:539	420 kDa	533:539	420 kDa	533:539	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	4	45	theme	active	475:480	arg1	weight					504:509	The active F2 fraction molecular weight	471:509	The active F2 fraction molecular weight of SP	471:515	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	1	46	theme	Marine	117:122	arg1	seaweeds					124:131	Marine seaweeds	117:131	Marine seaweeds	117:131	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	0	47	theme	sulfated	48:55	arg1	polysaccharide					57:70	sulfated polysaccharide	48:70	sulfated polysaccharide from Acanthophora spicifera (Vahl)	48:105	Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson.
29337104	4	48	theme	molecular	494:502	arg1	420 kDa					533:539	420 kDa	533:539	420 kDa	533:539	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	4	48	theme	molecular	494:502	arg1	weight					504:509	The active F2 fraction molecular weight	471:509	The active F2 fraction molecular weight of SP	471:515	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	6	49	from	125 μg/mL	763:771	arg1	activity					742:749	reducing power activity	727:749	reducing power activity	727:749	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	6	49	from	125 μg/mL	763:771	arg1	150 μg/mL					713:721	150 μg/mL	713:721	150 μg/mL	713:721	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	7	50	theme	fraction	834:841	arg1	polysaccharide					818:831	the purified sulfated polysaccharide	796:831	the purified sulfated polysaccharide (fraction F2)	796:845	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	7	50	theme	fraction	834:841	arg1	F2					843:844	fraction F2	834:844	fraction F2	834:844	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	2	51	dep	FT-IR	306:310	arg1	analysis					331:338	analysis	331:338	analysis	331:338	The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis.
29337104	4	52	theme	fraction	485:492	arg1	420 kDa					533:539	420 kDa	533:539	420 kDa	533:539	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	4	52	theme	fraction	485:492	arg1	weight					504:509	The active F2 fraction molecular weight	471:509	The active F2 fraction molecular weight of SP	471:515	The active F2 fraction molecular weight of SP was found to be 420 kDa.
29337104	7	53	theme	A549	966:969	arg1	lines					976:980	A549 cell lines	966:980	A549 cell lines under in vitro condition	966:1005	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	1	54	theme	effective	225:233	arg1	activity					246:253	effective anticancer activity	225:253	effective anticancer activity	225:253	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	6	55	theme	scavenging	680:689	arg1	activity					691:698	DPPH free radical scavenging activity	662:698	DPPH free radical scavenging activity of 55.55%	662:708	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	3	56	theme	total	442:446	arg1	acid					455:458	total uranic acid	442:458	total uranic acid	442:458	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	1	57	theme	anticancer	235:244	arg1	activity					246:253	effective anticancer activity	225:253	effective anticancer activity	225:253	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	6	58	theme	radical	672:678	arg1	activity					691:698	DPPH free radical scavenging activity	662:698	DPPH free radical scavenging activity of 55.55%	662:708	Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL.
29337104	2	59	theme	structural	260:269	arg1	feature					271:277	The structural feature	256:277	The structural feature of SPs	256:284	The structural feature of SPs was studied through FT-IR and 1H NMR spectra analysis.
29337104	1	60	theme	valuable	143:150	arg1	source					152:157	a valuable source	141:157	a valuable source of functional bioactive polysaccharide	141:196	Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity.
29337104	3	61	theme	total	415:419	arg1	sulfate					421:427	total sulfate	415:427	total sulfate	415:427	The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found.
29337104	8	62	theme	polysaccharide	1014:1027	arg1	fractions					1029:1037	These polysaccharide fractions	1008:1037	These polysaccharide fractions	1008:1037	These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
29337104	7	63	theme	purified	800:807	arg1	polysaccharide					818:831	the purified sulfated polysaccharide	796:831	the purified sulfated polysaccharide (fraction F2)	796:845	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
29337104	7	63	theme	purified	800:807	arg1	F2					843:844	fraction F2	834:844	fraction F2	834:844	In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition.
30861449	0	0	theme	acetic	94:99	arg1	acid					101:104	acetic acid	94:104	acetic acid production	94:115	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	2	1	from	changes	419:425	arg1	composition					438:448	VFA composition	434:448	VFA composition	434:448	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	2	1	from	changes	419:425	arg1	structure					478:486	the microbial community structure	454:486	the microbial community structure	454:486	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	7	2	theme	separation	1001:1010	arg1	application					973:983	the application	969:983	the application of the membrane separation	969:1010	However, surprisingly, another polysaccharide using genus, Parabacteroides, did not benefit from the application of the membrane separation, suggesting more complicated mechanisms might be involved.
30861449	1	3	theme	interacting	251:261	arg1	acids					234:238	volatile fatty acids	219:238	volatile fatty acids (VFAs)	219:245	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	3	theme	interacting	251:261	arg1	community					204:212	the microbial community	190:212	the microbial community	190:212	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	3	theme	interacting	251:261	arg1	factors					263:269	interacting factors	251:269	interacting factors in anaerobic fermentation	251:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	3	theme	interacting	251:261	arg1	Substrates					178:187	Substrates	178:187	Substrates	178:187	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	3	4	theme	more	553:556	arg1	polysaccharide					558:571	much more polysaccharide	548:571	much more polysaccharide	548:571	The membrane rejected much more polysaccharide than protein.
30861449	4	5	theme	acetic	610:615	arg1	production					591:600	VFA production	587:600	VFA production	587:600	VFA production, mainly acetic acid, was greatly enhanced by the membrane separation.
30861449	4	5	theme	acetic	610:615	arg1	acid					617:620	mainly acetic acid	603:620	mainly acetic acid	603:620	VFA production, mainly acetic acid, was greatly enhanced by the membrane separation.
30861449	0	6	theme	acid	101:104	arg1	production					106:115	acetic acid production	94:115	acetic acid production	94:115	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	0	7	from	benefits	58:65	arg1	bioreactor					142:151	an anaerobic membrane bioreactor	120:151	an anaerobic membrane bioreactor for sludge fermentation	120:175	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	5	8	theme	glycolytic	676:685	arg1	species					687:693	The glycolytic species	672:693	The glycolytic species Christensenella minuta	672:716	The glycolytic species Christensenella minuta was significantly enriched.
30861449	5	9	dep	species	687:693	arg1	minuta					711:716	minuta	711:716	minuta	711:716	The glycolytic species Christensenella minuta was significantly enriched.
30861449	7	10	theme	membrane	992:999	arg1	separation					1001:1010	the membrane separation	988:1010	the membrane separation	988:1010	However, surprisingly, another polysaccharide using genus, Parabacteroides, did not benefit from the application of the membrane separation, suggesting more complicated mechanisms might be involved.
30861449	2	11	theme	sludge	384:389	arg1	fermentation					401:412	sludge anaerobic fermentation	384:412	sludge anaerobic fermentation	384:412	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	2	12	theme	anaerobic	391:399	arg1	fermentation					401:412	sludge anaerobic fermentation	384:412	sludge anaerobic fermentation	384:412	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	0	13	theme	rejection	5:13	arg1	rate					15:18	High rejection rate	0:18	High rejection rate of polysaccharides by microfiltration	0:56	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	6	14	theme	polysaccharide	810:823	arg1	rate					802:805	the higher retention rate	781:805	the higher retention rate of polysaccharide	781:823	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	6	14	theme	polysaccharide	810:823	arg1	explanation					750:760	The explanation	746:760	The explanation	746:760	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	0	15	theme	sludge	157:162	arg1	fermentation					164:175	sludge fermentation	157:175	sludge fermentation	157:175	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	1	16	theme	microbial	194:202	arg1	acids					234:238	volatile fatty acids	219:238	volatile fatty acids (VFAs)	219:245	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	16	theme	microbial	194:202	arg1	community					204:212	the microbial community	190:212	the microbial community	190:212	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	16	theme	microbial	194:202	arg1	factors					263:269	interacting factors	251:269	interacting factors in anaerobic fermentation	251:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	16	theme	microbial	194:202	arg1	Substrates					178:187	Substrates	178:187	Substrates	178:187	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	0	17	theme	High	0:3	arg1	rate					15:18	High rejection rate	0:18	High rejection rate of polysaccharides by microfiltration	0:56	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	1	18	theme	anaerobic	274:282	arg1	fermentation					284:295	anaerobic fermentation	274:295	anaerobic fermentation	274:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	2	19	dep	composition	438:448	arg1	the					430:432	the	430:432	the	430:432	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	2	20	theme	VFA	434:436	arg1	composition					438:448	VFA composition	434:448	VFA composition	434:448	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	0	21	theme	membrane	133:140	arg1	bioreactor					142:151	an anaerobic membrane bioreactor	120:151	an anaerobic membrane bioreactor for sludge fermentation	120:175	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	4	22	theme	VFA	587:589	arg1	production					591:600	VFA production	587:600	VFA production	587:600	VFA production, mainly acetic acid, was greatly enhanced by the membrane separation.
30861449	4	22	theme	VFA	587:589	arg1	acid					617:620	mainly acetic acid	603:620	mainly acetic acid	603:620	VFA production, mainly acetic acid, was greatly enhanced by the membrane separation.
30861449	0	23	theme	anaerobic	123:131	arg1	bioreactor					142:151	an anaerobic membrane bioreactor	120:151	an anaerobic membrane bioreactor for sludge fermentation	120:175	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	4	24	theme	membrane	651:658	arg1	separation					660:669	the membrane separation	647:669	the membrane separation	647:669	VFA production, mainly acetic acid, was greatly enhanced by the membrane separation.
30861449	1	25	theme	volatile	219:226	arg1	VFAs					241:244	VFAs	241:244	VFAs	241:244	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	25	theme	volatile	219:226	arg1	acids					234:238	volatile fatty acids	219:238	volatile fatty acids (VFAs)	219:245	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	25	theme	volatile	219:226	arg1	community					204:212	the microbial community	190:212	the microbial community	190:212	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	25	theme	volatile	219:226	arg1	factors					263:269	interacting factors	251:269	interacting factors in anaerobic fermentation	251:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	25	theme	volatile	219:226	arg1	Substrates					178:187	Substrates	178:187	Substrates	178:187	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	2	26	theme	community	468:476	arg1	structure					478:486	the microbial community structure	454:486	the microbial community structure	454:486	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	0	27	theme	polysaccharides	23:37	arg1	rate					15:18	High rejection rate	0:18	High rejection rate of polysaccharides by microfiltration	0:56	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	6	28	theme	retention	792:800	arg1	rate					802:805	the higher retention rate	781:805	the higher retention rate of polysaccharide	781:823	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	6	28	theme	retention	792:800	arg1	explanation					750:760	The explanation	746:760	The explanation	746:760	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	1	29	theme	fatty	228:232	arg1	VFAs					241:244	VFAs	241:244	VFAs	241:244	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	29	theme	fatty	228:232	arg1	acids					234:238	volatile fatty acids	219:238	volatile fatty acids (VFAs)	219:245	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	29	theme	fatty	228:232	arg1	community					204:212	the microbial community	190:212	the microbial community	190:212	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	29	theme	fatty	228:232	arg1	factors					263:269	interacting factors	251:269	interacting factors in anaerobic fermentation	251:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	1	29	theme	fatty	228:232	arg1	Substrates					178:187	Substrates	178:187	Substrates	178:187	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	2	30	theme	microbial	458:466	arg1	structure					478:486	the microbial community structure	454:486	the microbial community structure	454:486	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	6	31	theme	higher	785:790	arg1	rate					802:805	the higher retention rate	781:805	the higher retention rate of polysaccharide	781:823	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	6	31	theme	higher	785:790	arg1	explanation					750:760	The explanation	746:760	The explanation	746:760	The explanation was inferred to be the higher retention rate of polysaccharide compared with proteins by the microfiltration.
30861449	1	32	from	factors	263:269	arg1	fermentation					284:295	anaerobic fermentation	274:295	anaerobic fermentation	274:295	Substrates, the microbial community, and volatile fatty acids (VFAs) are interacting factors in anaerobic fermentation.
30861449	7	33	theme	complicated	1029:1039	arg1	mechanisms					1041:1050	more complicated mechanisms	1024:1050	more complicated mechanisms	1024:1050	However, surprisingly, another polysaccharide using genus, Parabacteroides, did not benefit from the application of the membrane separation, suggesting more complicated mechanisms might be involved.
30861449	2	34	theme	microfiltration	315:329	arg1	membrane					331:338	a microfiltration membrane	313:338	a microfiltration membrane	313:338	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
30861449	0	35	theme	Christensenella	67:81	arg1	minuta					83:88	Christensenella minuta	67:88	Christensenella minuta	67:88	High rejection rate of polysaccharides by microfiltration benefits Christensenella minuta and acetic acid production in an anaerobic membrane bioreactor for sludge fermentation.
30861449	2	36	theme	conventional	359:370	arg1	reactor					372:378	a conventional reactor	357:378	a conventional reactor for sludge anaerobic fermentation	357:412	In this paper, a microfiltration membrane was installed in a conventional reactor for sludge anaerobic fermentation, and changes in the VFA composition and the microbial community structure after the application were monitored.
31479237	1	0	theme	capsular	214:221	arg1	assemblies					223:232	oil-filled hierarchical capsular assemblies	190:232	oil-filled hierarchical capsular assemblies	190:232	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	2	1	theme	distribution	593:604	arg1	control					525:531	a precise control	515:531	a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks	515:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	3	2	theme	nanocarriers	802:813	arg1	range					769:773	a range	767:773	a range of functional multilayered nanocarriers of interest for biomedical applications	767:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	1	3	theme	assemblies	223:232	arg1	construction					174:185	a one-pot construction	164:185	a one-pot construction of oil-filled hierarchical capsular assemblies	164:232	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	3	4	theme	polysaccharides	734:748	arg1	library					705:711	a library	703:711	a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications	703:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	5	theme	polymer	388:394	arg1	combinations					396:407	polymer combinations	388:407	polymer combinations	388:407	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	3	6	dep	simplicity	634:643	arg1	The					630:632	The	630:632	The	630:632	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	7	theme	nanocapsules	496:507	arg1	class					453:457	a new class	447:457	a new class of mixed/multilayered multicomponent nanocapsules	447:507	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	8	theme	blocks	622:627	arg1	distribution					593:604	spatial distribution	585:604	spatial distribution of the building blocks	585:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	8	theme	blocks	622:627	arg1	dimensions					540:549	the dimensions	536:549	the dimensions	536:549	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	8	theme	blocks	622:627	arg1	thickness/composition					558:578	shell thickness/composition	552:578	shell thickness/composition	552:578	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	3	9	theme	biomedical	831:840	arg1	applications					842:853	biomedical applications	831:853	biomedical applications	831:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	10	theme	spatial	585:591	arg1	distribution					593:604	spatial distribution	585:604	spatial distribution of the building blocks	585:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	11	theme	sequential	348:357	arg1	nanoprecipitations					359:376	simultaneous and/or sequential nanoprecipitations	328:376	simultaneous and/or sequential nanoprecipitations involving polymer combinations	328:407	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	12	theme	shell	552:556	arg1	thickness/composition					558:578	shell thickness/composition	552:578	shell thickness/composition	552:578	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	13	theme	building	613:620	arg1	blocks					622:627	the building blocks	609:627	the building blocks	609:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	1	14	theme	nanoprecipitation	244:260	arg1	technique					262:270	the nanoprecipitation technique	240:270	the nanoprecipitation technique	240:270	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	0	15	theme	Programmable	0:11	arg1	Construction					26:37	Programmable Hierarchical Construction	0:37	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide	0:74	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
31479237	3	16	theme	functional	778:787	arg1	nanocarriers					802:813	functional multilayered nanocarriers	778:813	functional multilayered nanocarriers of interest for biomedical applications	778:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	17	theme	multicomponent	481:494	arg1	nanocapsules					496:507	mixed/multilayered multicomponent nanocapsules	462:507	mixed/multilayered multicomponent nanocapsules	462:507	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	18	theme	dimensions	540:549	arg1	control					525:531	a precise control	515:531	a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks	515:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	3	19	theme	approach	668:675	arg1	tunability					649:658	tunability	649:658	tunability	649:658	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	3	19	theme	approach	668:675	arg1	simplicity					634:643	simplicity	634:643	simplicity	634:643	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	20	theme	new	449:451	arg1	class					453:457	a new class	447:457	a new class of mixed/multilayered multicomponent nanocapsules	447:507	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	21	theme	mixed/multilayered	462:479	arg1	nanocapsules					496:507	mixed/multilayered multicomponent nanocapsules	462:507	mixed/multilayered multicomponent nanocapsules	462:507	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	0	22	theme	Polysaccharide	61:74	arg1	Construction					26:37	Programmable Hierarchical Construction	0:37	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide	0:74	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
31479237	2	23	theme	precise	517:523	arg1	control					525:531	a precise control	515:531	a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks	515:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	0	24	theme	Mixed/Multilayered	42:59	arg1	Polysaccharide					61:74	Mixed/Multilayered Polysaccharide	42:74	Mixed/Multilayered Polysaccharide	42:74	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
31479237	2	25	theme	thickness/composition	558:578	arg1	control					525:531	a precise control	515:531	a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks	515:627	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	26	theme	multicomponent	284:297	arg1	diagrams					305:312	multicomponent phase diagrams	284:312	multicomponent phase diagrams	284:312	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	2	27	theme	phase	299:303	arg1	diagrams					305:312	multicomponent phase diagrams	284:312	multicomponent phase diagrams	284:312	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	1	28	theme	one-pot	166:172	arg1	construction					174:185	a one-pot construction	164:185	a one-pot construction of oil-filled hierarchical capsular assemblies	164:232	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	3	29	theme	multilayered	789:800	arg1	nanocarriers					802:813	functional multilayered nanocarriers	778:813	functional multilayered nanocarriers of interest for biomedical applications	778:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	3	30	theme	ionic	728:732	arg1	polysaccharides					734:748	neutral and ionic polysaccharides	716:748	neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications	716:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	2	31	theme	simultaneous	328:339	arg1	nanoprecipitations					359:376	simultaneous and/or sequential nanoprecipitations	328:376	simultaneous and/or sequential nanoprecipitations involving polymer combinations	328:407	Relying on multicomponent phase diagrams, we show that simultaneous and/or sequential nanoprecipitations involving polymer combinations can be precisely programmed to design a new class of mixed/multilayered multicomponent nanocapsules, with a precise control of the dimensions, shell thickness/composition, and spatial distribution of the building blocks.
31479237	1	32	theme	hierarchical	201:212	arg1	assemblies					223:232	oil-filled hierarchical capsular assemblies	190:232	oil-filled hierarchical capsular assemblies	190:232	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	0	33	theme	Nanoprecipitation	121:137	arg1	Steps					139:143	Simultaneous/Sequential Nanoprecipitation Steps	97:143	Simultaneous/Sequential Nanoprecipitation Steps	97:143	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
31479237	3	34	theme	neutral	716:722	arg1	polysaccharides					734:748	neutral and ionic polysaccharides	716:748	neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications	716:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	1	35	theme	oil-filled	190:199	arg1	assemblies					223:232	oil-filled hierarchical capsular assemblies	190:232	oil-filled hierarchical capsular assemblies	190:232	We report here on a one-pot construction of oil-filled hierarchical capsular assemblies using the nanoprecipitation technique.
31479237	0	36	theme	Simultaneous/Sequential	97:119	arg1	Steps					139:143	Simultaneous/Sequential Nanoprecipitation Steps	97:143	Simultaneous/Sequential Nanoprecipitation Steps	97:143	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
31479237	3	37	theme	interest	818:825	arg1	nanocarriers					802:813	functional multilayered nanocarriers	778:813	functional multilayered nanocarriers of interest for biomedical applications	778:853	The simplicity and tunability of this approach are exemplified here with a library of neutral and ionic polysaccharides giving access to a range of functional multilayered nanocarriers of interest for biomedical applications.
31479237	0	38	theme	Hierarchical	13:24	arg1	Construction					26:37	Programmable Hierarchical Construction	0:37	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide	0:74	Programmable Hierarchical Construction of Mixed/Multilayered Polysaccharide Nanocapsules through Simultaneous/Sequential Nanoprecipitation Steps.
30658141	12	0	theme	injectable	1937:1946	arg1	scaffold					1962:1969	this injectable composite gel scaffold	1932:1969	this injectable composite gel scaffold	1932:1969	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	12	1	dep	delivery	1982:1989	arg1	applications					2019:2030	applications	2019:2030	applications	2019:2030	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	2	2	theme	protein	412:418	arg1	system					429:434	a protein delivery system	410:434	a protein delivery system for soft tissue engineering	410:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	2	2	theme	protein	412:418	arg1	polysaccharides					391:405	natural polysaccharides	383:405	natural polysaccharides	383:405	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	9	3	theme	OAlg/N-Chi	1506:1515	arg1	hydrogels					1517:1525	the soft OAlg/N-Chi hydrogels	1497:1525	the soft OAlg/N-Chi hydrogels	1497:1525	The alginate microspheres can serve as a filler to toughen the soft OAlg/N-Chi hydrogels.
30658141	11	4	theme	lower	1801:1805	arg1	rate					1807:1810	significantly lower rate	1787:1810	significantly lower rate	1787:1810	Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone.
30658141	7	5	theme	swelling	1205:1212	arg1	ratio					1214:1218	swelling ratio	1205:1218	swelling ratio	1205:1218	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	2	6	theme	composite	330:338	arg1	hydrogel					340:347	a covalently cross-linked composite hydrogel	304:347	a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering	304:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	8	7	theme	mechanical	1322:1331	arg1	properties					1344:1353	mechanical and stable properties	1322:1353	mechanical and stable properties of gel scaffolds	1322:1370	The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres.
30658141	5	8	theme	cross-linking	947:959	arg1	technique					961:969	the emulsion cross-linking technique	934:969	the emulsion cross-linking technique	934:969	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	6	9	theme	Bull	972:975	arg1	Albumin					983:989	Bull Serum Albumin	972:989	Bull Serum Albumin (BSA)	972:995	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	6	9	theme	Bull	972:975	arg1	BSA					992:994	BSA	992:994	BSA	992:994	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	8	10	theme	stable	1337:1342	arg1	properties					1344:1353	mechanical and stable properties	1322:1353	mechanical and stable properties of gel scaffolds	1322:1370	The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres.
30658141	2	11	link	cross-linked	317:328	arg1	hydrogel					340:347	a covalently cross-linked composite hydrogel	304:347	a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering	304:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	1	12	theme	medical	216:222	arg1	delivery					275:282	drug delivery	270:282	drug delivery	270:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	12	theme	medical	216:222	arg1	applications					224:235	medical applications	216:235	medical applications including tissue engineering and drug delivery	216:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	12	theme	medical	216:222	arg1	engineering					254:264	tissue engineering	247:264	tissue engineering	247:264	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	5	13	theme	Meanwhile	792:800	arg1	microspheres					812:823	Meanwhile, gel-like microspheres	792:823	Meanwhile, gel-like microspheres	792:823	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	2	14	theme	embedded	349:356	arg1	hydrogel					340:347	a covalently cross-linked composite hydrogel	304:347	a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering	304:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	8	15	theme	scaffolds	1362:1370	arg1	properties					1344:1353	mechanical and stable properties	1322:1353	mechanical and stable properties of gel scaffolds	1322:1370	The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres.
30658141	7	16	theme	gelation	1155:1162	arg1	rate					1164:1167	gelation rate	1155:1167	gelation rate	1155:1167	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	17	theme	BSA	1246:1248	arg1	release					1250:1256	BSA release	1246:1256	BSA release	1246:1256	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	6	18	theme	gel	1028:1030	arg1	microspheres					1032:1043	alginate gel microspheres	1019:1043	alginate gel microspheres	1019:1043	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	0	19	theme	BSA	94:96	arg1	encapsulation					98:110	BSA encapsulation	94:110	BSA encapsulation	94:110	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	10	20	theme	composite	1551:1559	arg1	scaffolds					1565:1573	composite gel scaffolds	1551:1573	composite gel scaffolds containing 0.5 mL volume	1551:1598	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	5	21	theme	2-10 μm	858:864	arg1	diameter					846:853	a diameter	844:853	a diameter of 2-10 μm	844:864	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	2	22	with	embedded	349:356	arg1	microspheres					363:374	microspheres	363:374	microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering	363:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	0	23	theme	tissue	121:126	arg1	engineering					128:138	soft tissue engineering	116:138	soft tissue engineering	116:138	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	6	24	theme	OAlg/N-Chi	1080:1089	arg1	hydrogels					1091:1099	OAlg/N-Chi hydrogels	1080:1099	OAlg/N-Chi hydrogels	1080:1099	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	10	25	theme	0.5 mL	1586:1591	arg1	volume					1593:1598	0.5 mL volume	1586:1598	0.5 mL volume	1586:1598	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	3	26	theme	water-soluble	516:528	arg1	chitosan					530:537	chitosan	530:537	chitosan	530:537	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	7	27	theme	scaffolds	1275:1283	arg1	ratio					1214:1218	swelling ratio	1205:1218	swelling ratio	1205:1218	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	27	theme	scaffolds	1275:1283	arg1	release					1250:1256	BSA release	1246:1256	BSA release	1246:1256	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	27	theme	scaffolds	1275:1283	arg1	morphology					1170:1179	morphology	1170:1179	morphology	1170:1179	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	27	theme	scaffolds	1275:1283	arg1	properties					1193:1202	mechanical properties	1182:1202	mechanical properties	1182:1202	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	27	theme	scaffolds	1275:1283	arg1	degradation					1230:1240	in vitro degradation	1221:1240	in vitro degradation	1221:1240	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	7	27	theme	scaffolds	1275:1283	arg1	rate					1164:1167	gelation rate	1155:1167	gelation rate	1155:1167	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	10	28	theme	scaffolds	1565:1573	arg1	modulus					1540:1546	Compressive modulus	1528:1546	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres	1528:1614	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	2	29	theme	tissue	445:450	arg1	engineering					452:462	soft tissue engineering	440:462	soft tissue engineering	440:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	9	30	theme	alginate	1442:1449	arg1	filler					1479:1484	a filler	1477:1484	a filler	1477:1484	The alginate microspheres can serve as a filler to toughen the soft OAlg/N-Chi hydrogels.
30658141	9	30	theme	alginate	1442:1449	arg1	microspheres					1451:1462	The alginate microspheres	1438:1462	The alginate microspheres	1438:1462	The alginate microspheres can serve as a filler to toughen the soft OAlg/N-Chi hydrogels.
30658141	1	31	theme	Hydrogels	141:149	arg1	materials					186:194	very promising materials	171:194	very promising materials	171:194	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	31	theme	Hydrogels	141:149	arg1	scaffolds					157:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	3	32	theme	composite	484:492	arg1	hydrogel					494:501	This biodegradable composite hydrogel	465:501	This biodegradable composite hydrogel	465:501	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	11	33	theme	control	1831:1837	arg1	hydrogel					1839:1846	control hydrogel	1831:1846	control hydrogel	1831:1846	Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone.
30658141	4	34	theme	oxidized	752:759	arg1	OAlg					771:774	OAlg	771:774	OAlg	771:774	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	34	theme	oxidized	752:759	arg1	alginate					761:768	oxidized alginate	752:768	oxidized alginate (OAlg)	752:775	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	0	35	theme	embedded	59:66	arg1	microspheres					77:88	hydrogel embedded alginate microspheres	50:88	hydrogel embedded alginate microspheres	50:88	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	11	36	theme	controlled	1709:1718	arg1	release					1720:1726	the controlled release	1705:1726	the controlled release of BSA encapsulated within this composite hydrogels	1705:1778	Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone.
30658141	12	37	theme	protein	1974:1980	arg1	delivery					1982:1989	protein delivery	1974:1989	protein delivery	1974:1989	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	12	38	theme	soft	1995:1998	arg1	engineering					2007:2017	soft tissue engineering	1995:2017	soft tissue engineering	1995:2017	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	4	39	theme	aldehyde	701:708	arg1	N-Chi					741:745	N-Chi	741:745	N-Chi	741:745	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	39	theme	aldehyde	701:708	arg1	groups					710:715	amino and aldehyde groups	691:715	groups	710:715	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	39	theme	aldehyde	701:708	arg1	chitosan					731:738	N-succinyl chitosan	720:738	N-succinyl chitosan (N-Chi)	720:746	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	39	theme	aldehyde	701:708	arg1	alginate					761:768	oxidized alginate	752:768	oxidized alginate (OAlg)	752:775	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	39	theme	aldehyde	701:708	arg1	OAlg					771:774	OAlg	771:774	OAlg	771:774	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	10	40	theme	control	1656:1662	arg1	hydrogel					1664:1671	the control hydrogel	1652:1671	the control hydrogel	1652:1671	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	3	41	theme	cross-linking	606:618	arg1	agents					620:625	chemical cross-linking agents	597:625	chemical cross-linking agents	597:625	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	5	42	theme	sodium	881:886	arg1	alginate					888:895	sodium alginate	881:895	sodium alginate with Ca2+ in an aqueous emulsion	881:928	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	12	43	theme	composite	1948:1956	arg1	scaffold					1962:1969	this injectable composite gel scaffold	1932:1969	this injectable composite gel scaffold	1932:1969	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	2	44	theme	delivery	420:427	arg1	system					429:434	a protein delivery system	410:434	a protein delivery system for soft tissue engineering	410:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	2	44	theme	delivery	420:427	arg1	polysaccharides					391:405	natural polysaccharides	383:405	natural polysaccharides	383:405	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	9	45	theme	soft	1501:1504	arg1	hydrogels					1517:1525	the soft OAlg/N-Chi hydrogels	1497:1525	the soft OAlg/N-Chi hydrogels	1497:1525	The alginate microspheres can serve as a filler to toughen the soft OAlg/N-Chi hydrogels.
30658141	12	46	theme	potential	1903:1911	arg1	opportunity					1913:1923	a potential opportunity	1901:1923	a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications	1901:2030	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	5	47	with	alginate	888:895	arg1	Ca2+					902:905	Ca2+	902:905	Ca2+	902:905	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	4	48	theme	amino	691:695	arg1	N-Chi					741:745	N-Chi	741:745	N-Chi	741:745	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	48	theme	amino	691:695	arg1	groups					710:715	amino and aldehyde groups	691:715	groups	710:715	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	48	theme	amino	691:695	arg1	chitosan					731:738	N-succinyl chitosan	720:738	N-succinyl chitosan (N-Chi)	720:746	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	48	theme	amino	691:695	arg1	alginate					761:768	oxidized alginate	752:768	oxidized alginate (OAlg)	752:775	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	48	theme	amino	691:695	arg1	OAlg					771:774	OAlg	771:774	OAlg	771:774	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	7	49	theme	mechanical	1182:1191	arg1	properties					1193:1202	mechanical properties	1182:1202	mechanical properties	1182:1202	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	2	50	theme	cross-linked	317:328	arg1	hydrogel					340:347	a covalently cross-linked composite hydrogel	304:347	a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering	304:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	1	51	theme	wide	202:205	arg1	delivery					275:282	drug delivery	270:282	drug delivery	270:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	51	theme	wide	202:205	arg1	range					207:211	a wide range	200:211	a wide range of medical applications including tissue engineering and drug delivery	200:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	51	theme	wide	202:205	arg1	engineering					254:264	tissue engineering	247:264	tissue engineering	247:264	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	6	52	theme	Serum	977:981	arg1	Albumin					983:989	Bull Serum Albumin	972:989	Bull Serum Albumin (BSA)	972:995	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	6	52	theme	Serum	977:981	arg1	BSA					992:994	BSA	992:994	BSA	992:994	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	4	53	theme	alginate	761:768	arg1	N-Chi					741:745	N-Chi	741:745	N-Chi	741:745	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	53	theme	alginate	761:768	arg1	groups					710:715	amino and aldehyde groups	691:715	groups	710:715	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	53	theme	alginate	761:768	arg1	chitosan					731:738	N-succinyl chitosan	720:738	N-succinyl chitosan (N-Chi)	720:746	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	53	theme	alginate	761:768	arg1	alginate					761:768	oxidized alginate	752:768	oxidized alginate (OAlg)	752:775	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	53	theme	alginate	761:768	arg1	OAlg					771:774	OAlg	771:774	OAlg	771:774	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	1	54	theme	applications	224:235	arg1	delivery					275:282	drug delivery	270:282	drug delivery	270:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	54	theme	applications	224:235	arg1	range					207:211	a wide range	200:211	a wide range of medical applications including tissue engineering and drug delivery	200:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	54	theme	applications	224:235	arg1	engineering					254:264	tissue engineering	247:264	tissue engineering	247:264	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	4	55	theme	chitosan	731:738	arg1	N-Chi					741:745	N-Chi	741:745	N-Chi	741:745	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	55	theme	chitosan	731:738	arg1	groups					710:715	amino and aldehyde groups	691:715	groups	710:715	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	55	theme	chitosan	731:738	arg1	chitosan					731:738	N-succinyl chitosan	720:738	N-succinyl chitosan (N-Chi)	720:746	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	55	theme	chitosan	731:738	arg1	alginate					761:768	oxidized alginate	752:768	oxidized alginate (OAlg)	752:775	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	55	theme	chitosan	731:738	arg1	OAlg					771:774	OAlg	771:774	OAlg	771:774	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	10	56	contain	containing	1575:1584	arg1	scaffolds					1565:1573	composite gel scaffolds	1551:1573	composite gel scaffolds containing 0.5 mL volume	1551:1598	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	10	56	contain	containing	1575:1584	arg2	volume					1593:1598	0.5 mL volume	1586:1598	0.5 mL volume	1586:1598	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	8	57	theme	gel	1358:1360	arg1	scaffolds					1362:1370	gel scaffolds	1358:1370	gel scaffolds	1358:1370	The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres.
30658141	7	58	theme	current	1141:1147	arg1	work					1149:1152	the current work	1137:1152	the current work	1137:1152	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	1	59	theme	based	151:155	arg1	materials					186:194	very promising materials	171:194	very promising materials	171:194	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	59	theme	based	151:155	arg1	scaffolds					157:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	6	60	theme	alginate	1019:1026	arg1	microspheres					1032:1043	alginate gel microspheres	1019:1043	alginate gel microspheres	1019:1043	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	5	61	theme	aqueous	913:919	arg1	emulsion					921:928	an aqueous emulsion	910:928	an aqueous emulsion	910:928	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	1	62	theme	promising	176:184	arg1	materials					186:194	very promising materials	171:194	very promising materials	171:194	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	1	62	theme	promising	176:184	arg1	scaffolds					157:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds	141:165	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	6	63	theme	composite	1114:1122	arg1	scaffold					1124:1131	a composite scaffold	1112:1131	a composite scaffold	1112:1131	Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold.
30658141	0	64	theme	soft	116:119	arg1	engineering					128:138	soft tissue engineering	116:138	soft tissue engineering	116:138	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	5	65	from	alginate	888:895	arg1	emulsion					921:928	an aqueous emulsion	910:928	an aqueous emulsion	910:928	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	5	66	dep	Meanwhile	792:800	arg1	gel-like					803:810	gel-like	803:810	gel-like	803:810	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	8	67	theme	alginate	1415:1422	arg1	microspheres					1424:1435	alginate microspheres	1415:1435	alginate microspheres	1415:1435	The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres.
30658141	10	68	theme	Compressive	1528:1538	arg1	modulus					1540:1546	Compressive modulus	1528:1546	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres	1528:1614	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	5	69	theme	emulsion	938:945	arg1	technique					961:969	the emulsion cross-linking technique	934:969	the emulsion cross-linking technique	934:969	Meanwhile, gel-like microspheres were prepared with a diameter of 2-10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique.
30658141	7	70	theme	composite	1265:1273	arg1	scaffolds					1275:1283	the composite scaffolds	1261:1283	the composite scaffolds	1261:1283	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	10	71	theme	gel	1561:1563	arg1	scaffolds					1565:1573	composite gel scaffolds	1551:1573	composite gel scaffolds containing 0.5 mL volume	1551:1598	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	7	72	dep	in	1221:1222	arg1	vitro					1224:1228	vitro	1224:1228	vitro	1224:1228	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	2	73	theme	soft	440:443	arg1	engineering					452:462	soft tissue engineering	440:462	soft tissue engineering	440:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	0	74	theme	hydrogel	50:57	arg1	microspheres					77:88	hydrogel embedded alginate microspheres	50:88	hydrogel embedded alginate microspheres	50:88	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	4	75	theme	Schiff-base	662:672	arg1	reaction					674:681	the Schiff-base reaction	658:681	the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively	658:789	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	10	76	theme	microspheres	1603:1614	arg1	modulus					1540:1546	Compressive modulus	1528:1546	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres	1528:1614	Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres.
30658141	0	77	theme	alginate	68:75	arg1	microspheres					77:88	hydrogel embedded alginate microspheres	50:88	hydrogel embedded alginate microspheres	50:88	Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering.
30658141	1	78	theme	tissue	247:252	arg1	engineering					254:264	tissue engineering	247:264	tissue engineering	247:264	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	3	79	theme	alginate	543:550	arg1	derivatives					552:562	alginate derivatives	543:562	alginate derivatives	543:562	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	7	80	theme	in	1221:1222	arg1	degradation					1230:1240	in vitro degradation	1221:1240	in vitro degradation	1221:1240	In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined.
30658141	12	81	theme	gel	1958:1960	arg1	scaffold					1962:1969	this injectable composite gel scaffold	1932:1969	this injectable composite gel scaffold	1932:1969	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	3	82	theme	biodegradable	470:482	arg1	hydrogel					494:501	This biodegradable composite hydrogel	465:501	This biodegradable composite hydrogel	465:501	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	3	83	theme	chemical	597:604	arg1	agents					620:625	chemical cross-linking agents	597:625	chemical cross-linking agents	597:625	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30658141	12	84	theme	tissue	2000:2005	arg1	engineering					2007:2017	soft tissue engineering	1995:2017	soft tissue engineering	1995:2017	These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.
30658141	11	85	theme	BSA	1731:1733	arg1	release					1720:1726	the controlled release	1705:1726	the controlled release of BSA encapsulated within this composite hydrogels	1705:1778	Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone.
30658141	2	86	theme	natural	383:389	arg1	system					429:434	a protein delivery system	410:434	a protein delivery system for soft tissue engineering	410:462	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	2	86	theme	natural	383:389	arg1	polysaccharides					391:405	natural polysaccharides	383:405	natural polysaccharides	383:405	This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering.
30658141	1	87	theme	drug	270:273	arg1	delivery					275:282	drug delivery	270:282	drug delivery	270:282	Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery.
30658141	4	88	theme	N-succinyl	720:729	arg1	N-Chi					741:745	N-Chi	741:745	N-Chi	741:745	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	4	88	theme	N-succinyl	720:729	arg1	chitosan					731:738	N-succinyl chitosan	720:738	N-succinyl chitosan (N-Chi)	720:746	The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively.
30658141	11	89	theme	composite	1760:1768	arg1	hydrogels					1770:1778	this composite hydrogels	1755:1778	this composite hydrogels	1755:1778	Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone.
30658141	3	90	theme	agents	620:625	arg1	addition					585:592	addition	585:592	addition of chemical cross-linking agents	585:625	This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents.
30447362	6	0	theme	PHB	921:923	arg1	granules					925:932	PHB granules	921:932	PHB granules	921:932	SEM showed a homogeneous distribution of PHB granules in the TPS matrix.
30447362	1	1	theme	thermoplastic	232:244	arg1	cornstarch					246:255	thermoplastic cornstarch	232:255	thermoplastic cornstarch (TPS) produced by the thermal compression molding method	232:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	1	1	theme	thermoplastic	232:244	arg1	TPS					258:260	TPS	258:260	TPS	258:260	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	0	2	theme	poly	96:99	arg1	PHB					119:121	plasma-treated poly (hydroxybutyrate) PHB	81:121	plasma-treated poly (hydroxybutyrate) PHB	81:121	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	8	3	theme	thermal	1327:1333	arg1	stability					1335:1343	a higher thermal stability	1318:1343	a higher thermal stability	1318:1343	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	2	4	theme	physical	330:337	arg1	properties					352:361	the physical and chemical properties	326:361	the physical and chemical properties	326:361	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	8	5	theme	higher	1320:1325	arg1	stability					1335:1343	a higher thermal stability	1318:1343	a higher thermal stability	1318:1343	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	7	6	theme	stress	1133:1138	arg1	concentrators					1140:1152	stress concentrators	1133:1152	stress concentrators	1133:1152	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	1	7	from	effect	159:164	arg1	cornstarch					246:255	thermoplastic cornstarch	232:255	thermoplastic cornstarch (TPS) produced by the thermal compression molding method	232:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	1	7	from	effect	159:164	arg1	TPS					258:260	TPS	258:260	TPS	258:260	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	4	8	theme	atmospheric	651:661	arg1	gas					697:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	4	9	theme	plasma	628:633	arg1	treatment					635:643	plasma treatment	628:643	plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas	628:699	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	6	10	from	distribution	905:916	arg1	matrix					945:950	the TPS matrix	937:950	the TPS matrix	937:950	SEM showed a homogeneous distribution of PHB granules in the TPS matrix.
30447362	4	11	mod	modified	616:623	arg1	surface					585:591	the surface	581:591	the surface of the PHB granules	581:611	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	4	11	mod	modified	616:623	arg3	treatment					635:643	plasma treatment	628:643	plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas	628:699	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	7	12	theme	plasma-treated	1039:1052	arg1	PHB					1054:1056	plasma-treated PHB	1039:1056	plasma-treated PHB	1039:1056	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	7	13	theme	agglomerated	1070:1081	arg1	particles					1083:1091	the agglomerated particles	1066:1091	the agglomerated particles in the starch matrix, which represented stress concentrators	1066:1152	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	1	14	theme	addition	173:180	arg1	effect					159:164	the effect	155:164	the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method	155:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	2	15	theme	different	375:383	arg1	glycerol					396:403	glycerol	396:403	glycerol as the plasticizer (25, 30 and 35 wt%)	396:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	2	15	theme	different	375:383	arg1	amounts					385:391	different amounts	375:391	different amounts of glycerol as the plasticizer (25, 30 and 35 wt%)	375:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	7	16	theme	composites	995:1004	arg1	micrographs					976:986	the micrographs	972:986	the micrographs of the composites, using the high concentration of plasma-treated PHB,	972:1057	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	7	17	theme	other	960:964	arg1	hand					966:969	the other hand	956:969	the other hand	956:969	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	4	18	theme	air	663:665	arg1	gas					697:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	8	19	with	composites	1273:1282	arg1	PHB					1289:1291	PHB	1289:1291	PHB	1289:1291	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	6	20	theme	homogeneous	893:903	arg1	distribution					905:916	a homogeneous distribution	891:916	a homogeneous distribution of PHB granules in the TPS matrix	891:950	SEM showed a homogeneous distribution of PHB granules in the TPS matrix.
30447362	7	21	theme	high	1017:1020	arg1	concentration					1022:1034	the high concentration	1013:1034	the high concentration of plasma-treated PHB	1013:1056	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	7	22	theme	mechanical	1212:1221	arg1	properties					1223:1232	the poor mechanical properties	1203:1232	the poor mechanical properties	1203:1232	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	2	23	theme	TPS	366:368	arg1	properties					352:361	the physical and chemical properties	326:361	the physical and chemical properties	326:361	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	8	24	theme	TPS	1375:1377	arg1	TPS					1375:1377	TPS	1375:1377	TPS	1375:1377	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	8	24	theme	TPS	1375:1377	arg1	PHB					1393:1395	untreated PHB	1383:1395	untreated PHB	1383:1395	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	8	24	theme	TPS	1375:1377	arg1	composites					1361:1370	the composites	1357:1370	the composites of TPS and untreated PHB	1357:1395	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	7	25	from	particles	1083:1091	arg1	matrix					1107:1112	the starch matrix	1096:1112	the starch matrix	1096:1112	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	7	26	theme	weak	1166:1169	arg1	adhesion					1183:1190	weak interfacial adhesion	1166:1190	weak interfacial adhesion leading to the poor mechanical properties	1166:1232	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	5	27	theme	PHB	744:746	arg1	contents					748:755	the PHB contents	740:755	the PHB contents	740:755	In order to evaluate the influence of the PHB contents before and after the plasma treatment, the composites are characterized by FTIR, TGA, DSC, XRD, SEM, and mechanical tests.
30447362	7	28	theme	interfacial	1171:1181	arg1	adhesion					1183:1190	weak interfacial adhesion	1166:1190	weak interfacial adhesion leading to the poor mechanical properties	1166:1232	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	3	29	theme	35	492:493	arg1	%					494:494	%	494:494	%	494:494	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	1	30	theme	poly	185:188	arg1	PHB					208:210	poly (hydroxybutyrate) PHB	185:210	poly (hydroxybutyrate) PHB as reinforcement	185:227	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	7	31	theme	starch	1100:1105	arg1	matrix					1107:1112	the starch matrix	1096:1112	the starch matrix	1096:1112	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	3	32	theme	%	494:494	arg1	glycerol					496:503	35% glycerol	492:503	35% glycerol	492:503	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	2	33	theme	glycerol	396:403	arg1	glycerol					396:403	glycerol	396:403	glycerol as the plasticizer (25, 30 and 35 wt%)	396:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	2	33	theme	glycerol	396:403	arg1	amounts					385:391	different amounts	375:391	different amounts of glycerol as the plasticizer (25, 30 and 35 wt%)	375:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	0	34	theme	starch	50:55	arg1	composite					57:65	thermoplastic starch composite	36:65	thermoplastic starch composite	36:65	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	5	35	theme	plasma	778:783	arg1	treatment					785:793	the plasma treatment	774:793	the plasma treatment	774:793	In order to evaluate the influence of the PHB contents before and after the plasma treatment, the composites are characterized by FTIR, TGA, DSC, XRD, SEM, and mechanical tests.
30447362	1	36	theme	hydroxybutyrate	191:205	arg1	PHB					208:210	poly (hydroxybutyrate) PHB	185:210	poly (hydroxybutyrate) PHB as reinforcement	185:227	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	0	37	theme	thermoplastic	36:48	arg1	composite					57:65	thermoplastic starch composite	36:65	thermoplastic starch composite	36:65	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	1	38	theme	thermal	279:285	arg1	method					307:312	the thermal compression molding method	275:312	the thermal compression molding method	275:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	7	39	theme	PHB	1054:1056	arg1	concentration					1022:1034	the high concentration	1013:1034	the high concentration of plasma-treated PHB	1013:1056	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	1	40	theme	compression	287:297	arg1	method					307:312	the thermal compression molding method	275:312	the thermal compression molding method	275:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	8	41	theme	composites	1273:1282	arg1	analysis					1257:1264	The thermogravimetric analysis	1235:1264	The thermogravimetric analysis of the composites with PHB treated by plasma	1235:1309	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	3	42	theme	different	509:517	arg1	contents					523:530	different PHB contents	509:530	different PHB contents (10, 20 and 30 wt%)	509:550	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	3	42	theme	different	509:517	arg1	%					549:549	10, 20 and 30 wt%	533:549	10, 20 and 30 wt%	533:549	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	4	43	theme	PHB	600:602	arg1	granules					604:611	the PHB granules	596:611	the PHB granules	596:611	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	0	44	theme	composite	57:65	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	0	44	theme	composite	57:65	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	1	45	theme	molding	299:305	arg1	method					307:312	the thermal compression molding method	275:312	the thermal compression molding method	275:312	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	7	46	dep	composites	995:1004	arg1	using					1007:1011	using	1007:1011	using the high concentration of plasma-treated PHB	1007:1056	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	5	47	theme	mechanical	862:871	arg1	tests					873:877	mechanical tests	862:877	mechanical tests	862:877	In order to evaluate the influence of the PHB contents before and after the plasma treatment, the composites are characterized by FTIR, TGA, DSC, XRD, SEM, and mechanical tests.
30447362	3	48	theme	PHB	519:521	arg1	contents					523:530	different PHB contents	509:530	different PHB contents (10, 20 and 30 wt%)	509:550	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	3	48	theme	PHB	519:521	arg1	%					549:549	10, 20 and 30 wt%	533:549	10, 20 and 30 wt%	533:549	Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared.
30447362	1	49	theme	present	128:134	arg1	work					136:139	The present work	124:139	The present work	124:139	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	4	50	theme	hexafluoride	678:689	arg1	gas					697:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	atmospheric air and sulfur hexafluoride (SF6) gas	651:699	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	6	51	theme	TPS	941:943	arg1	matrix					945:950	the TPS matrix	937:950	the TPS matrix	937:950	SEM showed a homogeneous distribution of PHB granules in the TPS matrix.
30447362	1	52	theme	PHB	208:210	arg1	addition					173:180	the addition	169:180	the addition of poly (hydroxybutyrate) PHB as reinforcement	169:227	The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method.
30447362	4	53	theme	sulfur	671:676	arg1	SF6					692:694	SF6	692:694	SF6	692:694	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	4	53	theme	sulfur	671:676	arg1	hexafluoride					678:689	sulfur hexafluoride	671:689	sulfur hexafluoride (SF6)	671:695	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	4	54	theme	granules	604:611	arg1	surface					585:591	the surface	581:591	the surface of the PHB granules	581:611	Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6) gas.
30447362	0	55	theme	plasma-treated	81:94	arg1	PHB					119:121	plasma-treated poly (hydroxybutyrate) PHB	81:121	plasma-treated poly (hydroxybutyrate) PHB	81:121	Preparation and characterization of thermoplastic starch composite reinforced by plasma-treated poly (hydroxybutyrate) PHB.
30447362	6	56	theme	granules	925:932	arg1	distribution					905:916	a homogeneous distribution	891:916	a homogeneous distribution of PHB granules in the TPS matrix	891:950	SEM showed a homogeneous distribution of PHB granules in the TPS matrix.
30447362	5	57	theme	contents	748:755	arg1	influence					727:735	the influence	723:735	the influence of the PHB contents before and after the plasma treatment	723:793	In order to evaluate the influence of the PHB contents before and after the plasma treatment, the composites are characterized by FTIR, TGA, DSC, XRD, SEM, and mechanical tests.
30447362	8	58	theme	PHB	1393:1395	arg1	TPS					1375:1377	TPS	1375:1377	TPS	1375:1377	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	8	58	theme	PHB	1393:1395	arg1	PHB					1393:1395	untreated PHB	1383:1395	untreated PHB	1383:1395	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	8	58	theme	PHB	1393:1395	arg1	composites					1361:1370	the composites	1357:1370	the composites of TPS and untreated PHB	1357:1395	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	2	59	with	TPS	366:368	arg1	glycerol					396:403	glycerol	396:403	glycerol as the plasticizer (25, 30 and 35 wt%)	396:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	2	59	with	TPS	366:368	arg1	amounts					385:391	different amounts	375:391	different amounts of glycerol as the plasticizer (25, 30 and 35 wt%)	375:442	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	8	60	theme	thermogravimetric	1239:1255	arg1	analysis					1257:1264	The thermogravimetric analysis	1235:1264	The thermogravimetric analysis of the composites with PHB treated by plasma	1235:1309	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
30447362	2	61	theme	chemical	343:350	arg1	properties					352:361	the physical and chemical properties	326:361	the physical and chemical properties	326:361	Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated.
30447362	7	62	theme	poor	1207:1210	arg1	properties					1223:1232	the poor mechanical properties	1203:1232	the poor mechanical properties	1203:1232	On the other hand, the micrographs of the composites, using the high concentration of plasma-treated PHB, showed the agglomerated particles in the starch matrix, which represented stress concentrators, and showed weak interfacial adhesion leading to the poor mechanical properties.
30447362	8	63	theme	untreated	1383:1391	arg1	PHB					1393:1395	untreated PHB	1383:1395	untreated PHB	1383:1395	The thermogravimetric analysis of the composites with PHB treated by plasma showed a higher thermal stability compared to the composites of TPS and untreated PHB.
31201910	2	0	located	found	389:393	arg2	fructose					376:383	only fructose	371:383	only fructose	371:383	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	2	0	located	found	389:393	arg1	structure					417:425	the repeating unit structure	398:425	the repeating unit structure	398:425	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	5	1	theme	levan	625:629	arg1	S81					631:633	The levan S81	621:633	The levan S81	621:633	The levan S81 showed high level of thermal stability determined by DSC and TGA analysis.
31201910	7	2	theme	immunomodulatory	946:961	arg1	role					963:966	immunomodulatory role	946:966	immunomodulatory role	946:966	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	3	theme	radical	1107:1113	arg1	test					1135:1138	hydroxyl radical scavenging activity test	1098:1138	hydroxyl radical scavenging activity test	1098:1138	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	4	theme	high	932:935	arg1	level					937:941	a high level	930:941	a high level of immunomodulatory role	930:966	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	2	5	theme	only	371:374	arg1	fructose					376:383	only fructose	371:383	only fructose	371:383	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	6	6	dep	showed	736:741	arg1	formed					800:805	formed	800:805	showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively	736:894	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	7	7	theme	hydroxyl	1098:1105	arg1	test					1135:1138	hydroxyl radical scavenging activity test	1098:1138	hydroxyl radical scavenging activity test	1098:1138	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	3	8	link	-linked	511:517	arg1	fructan					519:525	a β-(2 → 6)-linked fructan	500:525	a β-(2 → 6)-linked fructan	500:525	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	4	9	attach	presence	568:575	arg2	rings					593:597	the furanoid rings	580:597	the furanoid rings	580:597	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	4	9	attach	presence	568:575	arg1	structure					610:618	the EPS structure	602:618	the EPS structure	602:618	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	6	10	theme	aqueous	783:789	arg1	solution					791:798	its aqueous solution	779:798	its aqueous solution	779:798	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	8	11	dep	immunomodulatory	1266:1281	arg1	a					1246:1246	a	1246:1246	a	1246:1246	These findings reveal potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant.
31201910	0	12	theme	Leuconostoc	77:87	arg1	mesenteroides					89:101	Leuconostoc mesenteroides	77:101	Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities	77:154	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	6	13	theme	AFM	869:871	arg1	analysis					873:880	SEM and AFM analysis	861:880	analysis	873:880	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	5	14	theme	high	642:645	arg1	level					647:651	high level	642:651	high level of thermal stability determined by DSC and TGA analysis	642:707	The levan S81 showed high level of thermal stability determined by DSC and TGA analysis.
31201910	2	15	theme	EPS	312:314	arg1	composition					293:303	The monosaccharide composition	274:303	The monosaccharide composition of the EPS from strain S81	274:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	0	16	theme	immunostimulatory	111:127	arg1	activities					145:154	immunostimulatory and antioxidant activities	111:154	immunostimulatory and antioxidant activities	111:154	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	4	17	theme	EPS	606:608	arg1	structure					610:618	the EPS structure	602:618	the EPS structure	602:618	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	3	18	theme	-linked	511:517	arg1	fructan					519:525	a β-(2 → 6)-linked fructan	500:525	a β-(2 → 6)-linked fructan	500:525	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	3	19	theme	spectroscopy	436:447	arg1	analysis					449:456	The NMR spectroscopy analysis	428:456	The NMR spectroscopy analysis	428:456	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	2	20	theme	unit	412:415	arg1	structure					417:425	the repeating unit structure	398:425	the repeating unit structure	398:425	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	8	21	theme	S81	1208:1210	arg1	potential					1189:1197	potential	1189:1197	potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant	1189:1297	These findings reveal potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant.
31201910	6	22	theme	SEM	861:863	arg1	analysis					873:880	SEM and AFM analysis	861:880	analysis	873:880	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	8	23	theme	levan	1202:1206	arg1	S81					1208:1210	levan S81	1202:1210	levan S81	1202:1210	These findings reveal potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant.
31201910	7	24	theme	levan	913:917	arg1	S81					919:921	the levan S81	909:921	the levan S81	909:921	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	2	25	theme	repeating	402:410	arg1	structure					417:425	the repeating unit structure	398:425	the repeating unit structure	398:425	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	6	26	theme	compact	756:762	arg1	morphology					764:773	a sheet-like compact morphology	743:773	a sheet-like compact morphology	743:773	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	1	27	theme	traditional	205:215	arg1	sourdough					217:225	traditional sourdough	205:225	traditional sourdough	205:225	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	0	28	theme	Partial	0:6	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization of a levan type exopolysaccharide (EPS)	0:63	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	7	29	theme	EC50	1063:1066	arg1	value					1068:1072	EC50 value 1.7 mg mL-1	1063:1084	EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions	1063:1164	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	6	30	theme	sheet-like	745:754	arg1	morphology					764:773	a sheet-like compact morphology	743:773	a sheet-like compact morphology	743:773	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	4	31	from	presence	568:575	arg1	structure					610:618	the EPS structure	602:618	the EPS structure	602:618	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	7	32	theme	in	1146:1147	arg1	conditions					1155:1164	in vitro conditions	1146:1164	in vitro conditions	1146:1164	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	4	33	theme	FTIR	532:535	arg1	analysis					537:544	The FTIR analysis	528:544	The FTIR analysis	528:544	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	0	34	theme	antioxidant	133:143	arg1	activities					145:154	immunostimulatory and antioxidant activities	111:154	immunostimulatory and antioxidant activities	111:154	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	2	35	theme	strain	321:326	arg1	S81					328:330	strain S81	321:330	strain S81	321:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	2	36	from	composition	293:303	arg1	S81					328:330	strain S81	321:330	strain S81	321:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	3	37	theme	levan	482:486	arg1	type					488:491	a levan type	480:491	a levan type EPS as a β-(2 → 6)-linked fructan	480:525	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	3	37	theme	levan	482:486	arg1	EPS					472:474	EPS	472:474	EPS	472:474	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	0	38	theme	type	36:39	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization of a levan type exopolysaccharide (EPS)	0:63	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	1	39	attach	isolated	191:198	arg2	mesenteroides					169:181	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	39	attach	isolated	191:198	arg1	sourdough					217:225	traditional sourdough	205:225	traditional sourdough	205:225	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	39	attach	isolated	191:198	arg2	strain					266:271	an exopolysaccharide (EPS) producer strain	230:271	an exopolysaccharide (EPS) producer strain	230:271	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	40	theme	exopolysaccharide	233:249	arg1	mesenteroides					169:181	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	40	theme	exopolysaccharide	233:249	arg1	strain					266:271	an exopolysaccharide (EPS) producer strain	230:271	an exopolysaccharide (EPS) producer strain	230:271	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	0	41	theme	levan	30:34	arg1	type					36:39	a levan type exopolysaccharide (EPS)	28:63	a levan type exopolysaccharide (EPS)	28:63	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	3	42	theme	NMR	432:434	arg1	analysis					449:456	The NMR spectroscopy analysis	428:456	The NMR spectroscopy analysis	428:456	The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan.
31201910	6	43	theme	compact	831:837	arg1	structure					839:847	a compact structure	829:847	a compact structure detected by SEM and AFM analysis, respectively	829:894	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	7	44	theme	anti-inflammatory	981:997	arg1	IL-4					1008:1011	the anti-inflammatory cytokine IL-4	977:1011	the anti-inflammatory cytokine IL-4	977:1011	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	45	theme	scavenging	1115:1124	arg1	test					1135:1138	hydroxyl radical scavenging activity test	1098:1138	hydroxyl radical scavenging activity test	1098:1138	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	5	46	theme	TGA	696:698	arg1	analysis					700:707	TGA analysis	696:707	TGA analysis	696:707	The levan S81 showed high level of thermal stability determined by DSC and TGA analysis.
31201910	0	47	dep	type	36:39	arg1	EPS					60:62	EPS	60:62	EPS	60:62	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	0	47	dep	type	36:39	arg1	exopolysaccharide					41:57	exopolysaccharide	41:57	a levan type exopolysaccharide (EPS)	28:63	Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
31201910	7	48	theme	cytokine	999:1006	arg1	IL-4					1008:1011	the anti-inflammatory cytokine IL-4	977:1011	the anti-inflammatory cytokine IL-4	977:1011	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	49	theme	strong	1030:1035	arg1	capacity					1049:1056	a strong antioxidant capacity	1028:1056	a strong antioxidant capacity	1028:1056	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	50	theme	activity	1126:1133	arg1	test					1135:1138	hydroxyl radical scavenging activity test	1098:1138	hydroxyl radical scavenging activity test	1098:1138	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	7	51	theme	antioxidant	1037:1047	arg1	capacity					1049:1056	a strong antioxidant capacity	1028:1056	a strong antioxidant capacity	1028:1056	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	6	52	theme	levan	726:730	arg1	S81					732:734	The lyophilised levan S81	710:734	The lyophilised levan S81	710:734	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	7	53	dep	in	1146:1147	arg1	vitro					1149:1153	vitro	1149:1153	vitro	1149:1153	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	5	54	theme	thermal	656:662	arg1	stability					664:672	thermal stability	656:672	thermal stability determined by DSC and TGA analysis	656:707	The levan S81 showed high level of thermal stability determined by DSC and TGA analysis.
31201910	6	55	theme	lyophilised	714:724	arg1	S81					732:734	The lyophilised levan S81	710:734	The lyophilised levan S81	710:734	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	2	56	theme	HPLC	353:356	arg1	analysis					358:365	HPLC analysis	353:365	HPLC analysis	353:365	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	4	57	theme	rings	593:597	arg1	presence					568:575	the presence	564:575	the presence of the furanoid rings in the EPS structure	564:618	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	5	58	theme	stability	664:672	arg1	level					647:651	high level	642:651	high level of thermal stability determined by DSC and TGA analysis	642:707	The levan S81 showed high level of thermal stability determined by DSC and TGA analysis.
31201910	6	59	theme	spheroidal	807:816	arg1	lumps					818:822	spheroidal lumps	807:822	spheroidal lumps	807:822	The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively.
31201910	2	60	theme	monosaccharide	278:291	arg1	composition					293:303	The monosaccharide composition	274:303	The monosaccharide composition of the EPS from strain S81	274:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	7	61	theme	role	963:966	arg1	level					937:941	a high level	930:941	a high level of immunomodulatory role	930:966	Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL-1 obtained by hydroxyl radical scavenging activity test under in vitro conditions.
31201910	4	62	theme	furanoid	584:591	arg1	rings					593:597	the furanoid rings	580:597	the furanoid rings	580:597	The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure.
31201910	1	63	theme	Leuconostoc	157:167	arg1	mesenteroides					169:181	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	63	theme	Leuconostoc	157:167	arg1	strain					266:271	an exopolysaccharide (EPS) producer strain	230:271	an exopolysaccharide (EPS) producer strain	230:271	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	8	64	theme	technological	1216:1228	arg1	purposes					1230:1237	technological purposes	1216:1237	technological purposes	1216:1237	These findings reveal potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant.
31201910	1	65	theme	producer	257:264	arg1	mesenteroides					169:181	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81	157:185	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	1	65	theme	producer	257:264	arg1	strain					266:271	an exopolysaccharide (EPS) producer strain	230:271	an exopolysaccharide (EPS) producer strain	230:271	Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain.
31201910	2	66	from	S81	328:330	arg1	EPS					312:314	the EPS	308:314	the EPS from strain S81	308:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
31201910	2	66	from	S81	328:330	arg1	composition					293:303	The monosaccharide composition	274:303	The monosaccharide composition of the EPS from strain S81	274:330	The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure.
30783145	7	0	theme	infrared	1165:1172	arg1	spectroscopy					1174:1185	infrared spectroscopy	1165:1185	infrared spectroscopy	1165:1185	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	5	1	theme	wall	869:872	arg1	amount					874:879	cell wall amount	864:879	cell wall amount	864:879	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
30783145	2	2	theme	freezing	529:536	arg1	tolerance					538:546	freezing tolerance	529:546	freezing tolerance referred to as sub-zero acclimation (SZA)	529:588	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	3	3	theme	SZA	672:674	arg1	basis					663:667	the molecular basis	649:667	the molecular basis of SZA	649:674	There is comparatively little information available about the molecular basis of SZA.
30783145	0	4	from	modification	52:63	arg1	proteome					98:105	the extracellular proteome	80:105	the extracellular proteome in Arabidopsis thaliana	80:129	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	6	5	theme	wall	958:961	arg1	amount					963:968	cell wall amount	953:968	cell wall amount	953:968	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	0	6	theme	extracellular	84:96	arg1	proteome					98:105	the extracellular proteome	80:105	the extracellular proteome in Arabidopsis thaliana	80:129	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	8	7	theme	wall	1365:1368	arg1	enzymes					1380:1386	cell wall modifying enzymes	1360:1386	cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases	1360:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	7	theme	wall	1365:1368	arg1	endotransglucosylases/hydrolases					1468:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	7	theme	wall	1365:1368	arg1	inhibitors					1442:1451	pectin methylesterase inhibitors	1420:1451	pectin methylesterase inhibitors	1420:1451	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	7	theme	wall	1365:1368	arg1	methylesterases					1403:1417	pectin methylesterases	1396:1417	pectin methylesterases	1396:1417	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	6	8	theme	cell	953:956	arg1	amount					963:968	cell wall amount	953:968	cell wall amount	953:968	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	6	9	theme	significant	929:939	arg1	increase					941:948	a significant increase	927:948	a significant increase in cell wall amount	927:968	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	8	10	theme	proteomic	1318:1326	arg1	approach					1328:1335	a proteomic approach	1316:1335	a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix	1316:1527	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	7	11	theme	chromatography-mass	1239:1257	arg1	spectrometry					1259:1270	gas chromatography-mass spectrometry	1235:1270	gas chromatography-mass spectrometry	1235:1270	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	4	12	theme	important	768:776	arg1	role					778:781	an important role	765:781	an important role	765:781	However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA.
30783145	2	13	theme	many	371:374	arg1	thaliana					411:418	Arabidopsis thaliana	399:418	Arabidopsis thaliana	399:418	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	13	theme	many	371:374	arg1	species					382:388	many plant species	371:388	many plant species	371:388	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	7	14	from	changes	1127:1133	arg1	structure					1138:1146	structure	1138:1146	structure	1138:1146	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	2	15	from	increase	517:524	arg1	tolerance					538:546	freezing tolerance	529:546	freezing tolerance referred to as sub-zero acclimation (SZA)	529:588	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	16	theme	plant	376:380	arg1	thaliana					411:418	Arabidopsis thaliana	399:418	Arabidopsis thaliana	399:418	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	16	theme	plant	376:380	arg1	species					382:388	many plant species	371:388	many plant species	371:388	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	17	theme	mild	457:460	arg1	temperatures					485:496	mild, non-damaging freezing temperatures	457:496	mild, non-damaging freezing temperatures	457:496	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	4	18	theme	cell	733:736	arg1	modification					743:754	cell wall modification	733:754	cell wall modification	733:754	However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA.
30783145	1	19	theme	increased	163:171	arg1	tolerance					188:196	increased plant freezing tolerance	163:196	increased plant freezing tolerance	163:196	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	8	20	theme	extracellular	1508:1520	arg1	matrix					1522:1527	the extracellular matrix	1504:1527	the extracellular matrix	1504:1527	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	1	21	theme	plant	173:177	arg1	tolerance					188:196	increased plant freezing tolerance	163:196	increased plant freezing tolerance	163:196	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	0	22	theme	sub-zero	14:21	arg1	acclimation					23:33	sub-zero acclimation	14:33	sub-zero acclimation	14:33	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	1	23	theme	many	263:266	arg1	changes					309:315	many physiological, biochemical and molecular changes	263:315	many physiological, biochemical and molecular changes that have been extensively investigated	263:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	8	24	theme	xyloglucan	1457:1466	arg1	endotransglucosylases/hydrolases					1468:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	25	theme	modifying	1370:1378	arg1	enzymes					1380:1386	cell wall modifying enzymes	1360:1386	cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases	1360:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	25	theme	modifying	1370:1378	arg1	endotransglucosylases/hydrolases					1468:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	25	theme	modifying	1370:1378	arg1	inhibitors					1442:1451	pectin methylesterase inhibitors	1420:1451	pectin methylesterase inhibitors	1420:1451	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	25	theme	modifying	1370:1378	arg1	methylesterases					1403:1417	pectin methylesterases	1396:1417	pectin methylesterases	1396:1417	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	1	26	theme	freezing	179:186	arg1	tolerance					188:196	increased plant freezing tolerance	163:196	increased plant freezing tolerance	163:196	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	5	27	from	changes	853:859	arg1	composition					882:892	composition	882:892	composition	882:892	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
30783145	5	27	from	changes	853:859	arg1	amount					874:879	cell wall amount	864:879	cell wall amount	864:879	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
30783145	5	27	from	changes	853:859	arg1	structure					898:906	structure	898:906	structure	898:906	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
30783145	1	28	theme	physiological	268:280	arg1	changes					309:315	many physiological, biochemical and molecular changes	263:315	many physiological, biochemical and molecular changes that have been extensively investigated	263:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	0	29	theme	Arabidopsis	110:120	arg1	thaliana					122:129	Arabidopsis thaliana	110:129	Arabidopsis thaliana	110:129	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	3	30	theme	available	633:641	arg1	information					621:631	comparatively little information	600:631	comparatively little information available about the molecular basis of SZA	600:674	There is comparatively little information available about the molecular basis of SZA.
30783145	4	31	theme	wall	738:741	arg1	modification					743:754	cell wall modification	733:754	cell wall modification	733:754	However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA.
30783145	8	32	theme	cell	1360:1363	arg1	enzymes					1380:1386	cell wall modifying enzymes	1360:1386	cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases	1360:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	32	theme	cell	1360:1363	arg1	endotransglucosylases/hydrolases					1468:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	xyloglucan endotransglucosylases/hydrolases	1457:1499	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	32	theme	cell	1360:1363	arg1	inhibitors					1442:1451	pectin methylesterase inhibitors	1420:1451	pectin methylesterase inhibitors	1420:1451	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	8	32	theme	cell	1360:1363	arg1	methylesterases					1403:1417	pectin methylesterases	1396:1417	pectin methylesterases	1396:1417	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	6	33	from	increase	941:948	arg1	amount					963:968	cell wall amount	953:968	cell wall amount	953:968	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	0	34	from	changes	69:75	arg1	proteome					98:105	the extracellular proteome	80:105	the extracellular proteome in Arabidopsis thaliana	80:129	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	6	35	theme	pectin	999:1004	arg1	proportions					984:994	the relative proportions	971:994	the relative proportions of pectin, hemicellulose and cellulose	971:1033	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	1	36	theme	biochemical	283:293	arg1	changes					309:315	many physiological, biochemical and molecular changes	263:315	many physiological, biochemical and molecular changes that have been extensively investigated	263:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	5	37	theme	extensive	843:851	arg1	changes					853:859	extensive changes	843:859	extensive changes in cell wall amount, composition and structure	843:906	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
30783145	3	38	theme	little	614:619	arg1	information					621:631	comparatively little information	600:631	comparatively little information available about the molecular basis of SZA	600:674	There is comparatively little information available about the molecular basis of SZA.
30783145	8	39	theme	enzymes	1380:1386	arg1	accumulation					1344:1355	the accumulation	1340:1355	the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix	1340:1527	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	6	40	theme	cellulose	1025:1033	arg1	proportions					984:994	the relative proportions	971:994	the relative proportions of pectin, hemicellulose and cellulose	971:1033	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	0	41	theme	wall	47:50	arg1	modification					52:63	cell wall modification	42:63	cell wall modification	42:63	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	2	42	theme	additional	506:515	arg1	increase					517:524	an additional increase	503:524	an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA)	503:588	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	43	theme	sub-zero	563:570	arg1	SZA					585:587	SZA	585:587	SZA	585:587	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	2	43	theme	sub-zero	563:570	arg1	acclimation					572:582	sub-zero acclimation	563:582	sub-zero acclimation (SZA)	563:588	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	1	44	dep	low	217:219	arg1	non-freezing					222:233	non-freezing	222:233	non-freezing	222:233	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	8	45	theme	pectin	1396:1401	arg1	methylesterases					1403:1417	pectin methylesterases	1396:1417	pectin methylesterases	1396:1417	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	4	46	theme	transcriptomic	695:708	arg1	studies					710:716	previous transcriptomic studies	686:716	previous transcriptomic studies	686:716	However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA.
30783145	0	47	theme	cell	42:45	arg1	modification					52:63	cell wall modification	42:63	cell wall modification	42:63	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	1	48	theme	molecular	299:307	arg1	changes					309:315	many physiological, biochemical and molecular changes	263:315	many physiological, biochemical and molecular changes that have been extensively investigated	263:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	2	49	dep	mild	457:460	arg1	non-damaging					463:474	non-damaging	463:474	non-damaging	463:474	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	8	50	theme	pectin	1420:1425	arg1	inhibitors					1442:1451	pectin methylesterase inhibitors	1420:1451	pectin methylesterase inhibitors	1420:1451	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	4	51	theme	previous	686:693	arg1	studies					710:716	previous transcriptomic studies	686:716	previous transcriptomic studies	686:716	However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA.
30783145	8	52	from	accumulation	1344:1355	arg1	matrix					1522:1527	the extracellular matrix	1504:1527	the extracellular matrix	1504:1527	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	1	53	theme	changes	309:315	arg1	result					253:258	a result	251:258	a result of many physiological, biochemical and molecular changes that have been extensively investigated	251:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	0	54	from	proteome	98:105	arg1	thaliana					122:129	Arabidopsis thaliana	110:129	Arabidopsis thaliana	110:129	Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.
30783145	6	55	theme	relative	975:982	arg1	proportions					984:994	the relative proportions	971:994	the relative proportions of pectin, hemicellulose and cellulose	971:1033	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	7	56	theme	gas	1235:1237	arg1	spectrometry					1259:1270	gas chromatography-mass spectrometry	1235:1270	gas chromatography-mass spectrometry	1235:1270	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	6	57	theme	hemicellulose	1007:1019	arg1	proportions					984:994	the relative proportions	971:994	the relative proportions of pectin, hemicellulose and cellulose	971:1033	While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA.
30783145	1	58	theme	Cold	132:135	arg1	CA					150:151	CA	150:151	CA	150:151	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	1	58	theme	Cold	132:135	arg1	acclimation					137:147	Cold acclimation	132:147	Cold acclimation (CA)	132:152	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	1	59	theme	low	217:219	arg1	temperatures					235:246	low, non-freezing temperatures	217:246	low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated	217:355	Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated.
30783145	2	60	theme	freezing	476:483	arg1	temperatures					485:496	mild, non-damaging freezing temperatures	457:496	mild, non-damaging freezing temperatures	457:496	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	3	61	theme	molecular	653:661	arg1	basis					663:667	the molecular basis	649:667	the molecular basis of SZA	649:674	There is comparatively little information available about the molecular basis of SZA.
30783145	8	62	theme	methylesterase	1427:1440	arg1	inhibitors					1442:1451	pectin methylesterase inhibitors	1420:1451	pectin methylesterase inhibitors	1420:1451	These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
30783145	2	63	theme	Arabidopsis	399:409	arg1	thaliana					411:418	Arabidopsis thaliana	399:418	Arabidopsis thaliana	399:418	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	7	64	theme	monosaccharide	1191:1204	arg1	composition					1206:1216	monosaccharide composition	1191:1216	monosaccharide composition	1191:1216	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	2	65	theme	subsequent	434:443	arg1	exposure					445:452	a subsequent exposure	432:452	a subsequent exposure to mild, non-damaging freezing temperatures	432:496	In addition, many plant species, such as Arabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA).
30783145	7	66	theme	subtle	1120:1125	arg1	changes					1127:1133	more subtle changes	1115:1133	more subtle changes in structure	1115:1146	However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry.
30783145	5	67	theme	cell	864:867	arg1	amount					874:879	cell wall amount	864:879	cell wall amount	864:879	Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure.
29427682	4	0	theme	H	644:644	arg1	groups					652:657	K, Z, H and D groups	638:657	K, Z, H and D groups respectively	638:670	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	1	from	level	511:515	arg1	Bacteroidetes					550:562	Bacteroidetes	550:562	Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota	550:633	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	1	from	level	511:515	arg1	phylum					539:544	the most predominant phylum	518:544	the most predominant phylum	518:544	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	2	2	theme	sample	357:362	arg1	groups					364:369	the three sample groups	347:369	the three sample groups	347:369	Analysis showed significant structural changes in fecal microbiota among the three sample groups.
29427682	1	3	theme	illumina	247:254	arg1	sequencing					262:271	illumina PE250 sequencing	247:271	illumina PE250 sequencing	247:271	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	0	4	theme	PE250	93:97	arg1	sequencing					99:108	illumina PE250 sequencing	84:108	illumina PE250 sequencing	84:108	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	0	5	from	Effect	0:5	arg1	microbiota					52:61	intestinal microbiota	41:61	intestinal microbiota in mice	41:69	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	2	6	theme	significant	290:300	arg1	changes					313:319	significant structural changes	290:319	significant structural changes in fecal microbiota among the three sample groups	290:369	Analysis showed significant structural changes in fecal microbiota among the three sample groups.
29427682	6	7	theme	mice	1012:1015	arg1	microbiota					1021:1030	shaping mice gut microbiota	1004:1030	shaping mice gut microbiota	1004:1030	In conclusion, our results suggest that polysaccharide type and glycoside may contribute to shaping mice gut microbiota.
29427682	1	8	theme	PE250	256:260	arg1	sequencing					262:271	illumina PE250 sequencing	247:271	illumina PE250 sequencing	247:271	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	0	9	theme	illumina	84:91	arg1	sequencing					99:108	illumina PE250 sequencing	84:108	illumina PE250 sequencing	84:108	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	0	10	from	microbiota	52:61	arg1	mice					66:69	mice	66:69	mice	66:69	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	5	11	theme	Prevotellaceae	848:861	arg1	p<0.05					872:877	p<0.05	872:877	p<0.05	872:877	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	5	11	theme	Prevotellaceae	848:861	arg1	UCG-001					863:869	Prevotellaceae UCG-001	848:869	Prevotellaceae UCG-001 (p<0.05)	848:878	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	5	12	theme	Z	803:803	arg1	group					811:815	the Z and H group	799:815	group	811:815	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	5	13	theme	H	809:809	arg1	group					811:815	the Z and H group	799:815	group	811:815	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	1	14	from	seaweeds	146:153	arg1	polysaccharides					121:135	polysaccharides	121:135	polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera,	121:195	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	1	14	from	seaweeds	146:153	arg1	Effect					111:116	Effect	111:116	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice	111:228	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	4	15	theme	K	638:638	arg1	groups					652:657	K, Z, H and D groups	638:657	K, Z, H and D groups respectively	638:670	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	16	theme	microbiota	624:633	arg1	microbiota					624:633	the fecal microbiota	614:633	the fecal microbiota	614:633	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	16	theme	microbiota	624:633	arg1	%					582:582	58.76%	577:582	58.76%	577:582	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	16	theme	microbiota	624:633	arg1	%					590:590	73.39%	585:590	73.39%	585:590	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	16	theme	microbiota	624:633	arg1	%					598:598	75.38%	593:598	75.38%	593:598	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	16	theme	microbiota	624:633	arg1	%					609:609	64.40%	604:609	64.40% of the fecal microbiota	604:633	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	3	17	from	differences	395:405	arg1	composition					414:424	the composition	410:424	the composition of fecal microbiota	410:444	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	6	18	theme	polysaccharide	952:965	arg1	type					967:970	polysaccharide type	952:970	polysaccharide type	952:970	In conclusion, our results suggest that polysaccharide type and glycoside may contribute to shaping mice gut microbiota.
29427682	4	19	theme	fecal	618:622	arg1	microbiota					624:633	the fecal microbiota	614:633	the fecal microbiota	614:633	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	2	20	theme	fecal	324:328	arg1	microbiota					330:339	fecal microbiota	324:339	fecal microbiota	324:339	Analysis showed significant structural changes in fecal microbiota among the three sample groups.
29427682	3	21	theme	microbiota	435:444	arg1	composition					414:424	the composition	410:424	the composition of fecal microbiota	410:444	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	1	22	from	Effect	111:116	arg1	seaweeds					146:153	two seaweeds	142:153	two seaweeds	142:153	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	1	22	from	Effect	111:116	arg1	haitanensis					165:175	Porphyra haitanensis	156:175	Porphyra haitanensis	156:175	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	1	22	from	Effect	111:116	arg1	prolifera					186:194	Ulva prolifera	181:194	Ulva prolifera	181:194	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	1	22	from	Effect	111:116	arg1	microbiota					211:220	intestinal microbiota	200:220	intestinal microbiota in mice	200:228	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	5	23	theme	K	829:829	arg1	group					831:835	the K group	825:835	the K group	825:835	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	0	24	theme	seaweed	14:20	arg1	polysaccharides					22:36	two seaweed polysaccharides	10:36	two seaweed polysaccharides	10:36	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	6	25	theme	gut	1017:1019	arg1	microbiota					1021:1030	shaping mice gut microbiota	1004:1030	shaping mice gut microbiota	1004:1030	In conclusion, our results suggest that polysaccharide type and glycoside may contribute to shaping mice gut microbiota.
29427682	3	26	theme	phylum	472:477	arg1	levels					489:494	phylum and genus levels	472:494	phylum and genus levels	472:494	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	3	27	theme	significant	383:393	arg1	differences					395:405	significant differences	383:405	significant differences in the composition of fecal microbiota among the three groups	383:467	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	2	28	theme	structural	302:311	arg1	changes					313:319	significant structural changes	290:319	significant structural changes in fecal microbiota among the three sample groups	290:369	Analysis showed significant structural changes in fecal microbiota among the three sample groups.
29427682	1	29	theme	intestinal	200:209	arg1	microbiota					211:220	intestinal microbiota	200:220	intestinal microbiota in mice	200:228	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	0	30	theme	polysaccharides	22:36	arg1	Effect					0:5	Effect	0:5	Effect of two seaweed polysaccharides on intestinal microbiota in mice	0:69	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	3	31	theme	fecal	429:433	arg1	microbiota					435:444	fecal microbiota	429:444	fecal microbiota	429:444	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	4	32	from	Bacteroidetes	550:562	arg1	groups					652:657	K, Z, H and D groups	638:657	K, Z, H and D groups respectively	638:670	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	5	33	theme	Many	758:761	arg1	genera					763:768	Many genera	758:768	Many genera	758:768	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	1	34	from	microbiota	211:220	arg1	mice					225:228	mice	225:228	mice	225:228	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	4	35	theme	D	650:650	arg1	groups					652:657	K, Z, H and D groups	638:657	K, Z, H and D groups respectively	638:670	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	0	36	theme	intestinal	41:50	arg1	microbiota					52:61	intestinal microbiota	41:61	intestinal microbiota in mice	41:69	Effect of two seaweed polysaccharides on intestinal microbiota in mice evaluated by illumina PE250 sequencing.
29427682	4	37	theme	predominant	527:537	arg1	Bacteroidetes					550:562	Bacteroidetes	550:562	Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota	550:633	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	37	theme	predominant	527:537	arg1	phylum					539:544	the most predominant phylum	518:544	the most predominant phylum	518:544	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	1	38	theme	polysaccharides	121:135	arg1	Effect					111:116	Effect	111:116	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice	111:228	Effect of polysaccharides from two seaweeds, Porphyra haitanensis and Ulva prolifera, on intestinal microbiota in mice was evaluated by illumina PE250 sequencing.
29427682	6	39	theme	shaping	1004:1010	arg1	microbiota					1021:1030	shaping mice gut microbiota	1004:1030	shaping mice gut microbiota	1004:1030	In conclusion, our results suggest that polysaccharide type and glycoside may contribute to shaping mice gut microbiota.
29427682	3	40	theme	genus	483:487	arg1	levels					489:494	phylum and genus levels	472:494	phylum and genus levels	472:494	There were significant differences in the composition of fecal microbiota among the three groups at phylum and genus levels.
29427682	4	41	theme	Z	641:641	arg1	groups					652:657	K, Z, H and D groups	638:657	K, Z, H and D groups respectively	638:670	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	4	42	theme	phylum	504:509	arg1	level					511:515	the phylum level	500:515	the phylum level	500:515	At the phylum level, the most predominant phylum was Bacteroidetes contributing 58.76%, 73.39%, 75.38% and 64.40% of the fecal microbiota in K, Z, H and D groups respectively, followed by Firmicutes, contributing 37.61%, 23.99%, 21.87% and 30.82% respectively.
29427682	5	43	theme	Rikenellaceae	884:896	arg1	p<0.01					903:908	p<0.01	903:908	p<0.01	903:908	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	5	43	theme	Rikenellaceae	884:896	arg1	RC9					898:900	Rikenellaceae RC9	884:900	Rikenellaceae RC9 (p<0.01)	884:909	Many genera were significantly higher in the Z and H group than in the K group, including Prevotellaceae UCG-001 (p<0.05) and Rikenellaceae RC9 (p<0.01).
29427682	2	44	from	changes	313:319	arg1	microbiota					330:339	fecal microbiota	324:339	fecal microbiota	324:339	Analysis showed significant structural changes in fecal microbiota among the three sample groups.
31013876	4	0	theme	wall	923:926	arg1	materials					928:936	more suitable wall materials	909:936	more suitable wall materials	909:936	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	8	1	theme	spray-drying	1664:1675	arg1	technique					1677:1685	the spray-drying technique	1660:1685	the spray-drying technique	1660:1685	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	0	2	theme	Powders	213:219	arg1	Quality					190:196	the Quality	186:196	the Quality of Spray-Dried Powders	186:219	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	7	3	theme	milk	1476:1479	arg1	g					1524:1524	0.5 g	1520:1524	0.5 g of beta-cyclodextrin	1520:1545	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	3	theme	milk	1476:1479	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	3	theme	milk	1476:1479	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	3	theme	milk	1476:1479	arg1	g					1466:1466	10 g	1463:1466	10 g of skim milk	1463:1479	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	3	theme	milk	1476:1479	arg1	g					1487:1487	8.96 g	1482:1487	8.96 g of resistant-maltodextrin	1482:1513	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	6	4	theme	skim	1234:1237	arg1	milk					1239:1242	skim milk	1234:1242	skim milk (6-10 g)	1234:1251	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	6	4	theme	skim	1234:1237	arg1	g					1250:1250	6-10 g	1245:1250	6-10 g	1245:1250	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	3	5	from	rich	771:774	arg1	compounds					788:796	volatile compounds	779:796	volatile compounds	779:796	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	4	6	dep	elsholtzia	867:876	arg1	ketones					900:906	ketones	900:906	ketones	900:906	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	0	7	from	Impact	81:86	arg1	Quality					190:196	the Quality	186:196	the Quality of Spray-Dried Powders	186:219	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	0	8	theme	Skim	150:153	arg1	Milk					155:158	Skim Milk	150:158	Skim Milk	150:158	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	3	9	theme	different	618:626	arg1	materials					633:641	different wall materials	618:641	different wall materials	618:641	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	7	10	theme	mixture	1411:1417	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	10	theme	mixture	1411:1417	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	3	11	theme	essential	802:810	arg1	oil					812:814	essential oil	802:814	essential oil from Elsholtzia ciliata herb	802:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	6	12	theme	encapsulation	1327:1339	arg1	efficiency					1341:1350	the encapsulation efficiency	1323:1350	the encapsulation efficiency	1323:1350	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	3	13	theme	ciliata	832:838	arg1	herb					840:843	Elsholtzia ciliata herb	821:843	Elsholtzia ciliata herb	821:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	1	14	theme	various	356:362	arg1	conditions					378:387	various environmental conditions	356:387	various environmental conditions	356:387	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	3	15	theme	materials	633:641	arg1	effects					607:613	the effects	603:613	the effects of different wall materials	603:641	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	6	16	theme	mixture	1101:1107	arg1	design					1121:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	9	17	theme	pharmaceutical	1782:1795	arg1	forms					1797:1801	various pharmaceutical forms	1774:1801	various pharmaceutical forms	1774:1801	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	8	18	contain	had	1575:1577	arg1	agents					1568:1573	These encapsulating agents	1548:1573	These encapsulating agents	1548:1573	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	8	18	contain	had	1575:1577	arg2	performance					1586:1596	a good performance	1579:1596	a good performance	1579:1596	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	6	19	used	used	1132:1135	arg2	design					1121:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	0	20	theme	Resistant-Maltodextrin	91:112	arg1	Impact					81:86	Impact	81:86	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.	0:220	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	2	21	theme	low-soluble	546:556	arg1	compounds					558:566	low-soluble compounds	546:566	low-soluble compounds	546:566	Spray-drying increases the bioavailability of the natural active compounds and improves the solubility of low-soluble compounds.
31013876	1	22	from	conditions	378:387	arg1	pH					419:420	pH	419:420	pH	419:420	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	compounds					341:349	biologically active compounds	321:349	biologically active compounds	321:349	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	stabilization					289:301	stabilization	289:301	stabilization	289:301	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	oxidation					398:406	oxidation	398:406	oxidation	398:406	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	protection					307:316	protection	307:316	protection	307:316	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	temperature					427:437	temperature	427:437	temperature	427:437	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	22	from	conditions	378:387	arg1	moisture					409:416	moisture	409:416	moisture	409:416	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	6	23	theme	wall	1163:1166	arg1	material					1168:1175	wall material	1163:1175	wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g)	1163:1317	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	3	24	from	compounds	788:796	arg1	rich					771:774	rich	771:774	rich	771:774	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	5	25	theme	skim	1021:1024	arg1	milk					1026:1029	skim milk	1021:1029	skim milk	1021:1029	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	6	26	dep	material	1168:1175	arg1	composition					1188:1198	solution's composition	1177:1198	wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g)	1163:1317	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	0	27	theme	Elsholtzia	22:31	arg1	Extract					56:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	2	28	theme	compounds	505:513	arg1	bioavailability					467:481	the bioavailability	463:481	the bioavailability of the natural active compounds	463:513	Spray-drying increases the bioavailability of the natural active compounds and improves the solubility of low-soluble compounds.
31013876	0	29	theme	Herb	41:44	arg1	Extract					56:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	7	30	theme	beta-cyclodextrin	1529:1545	arg1	g					1524:1524	0.5 g	1520:1524	0.5 g of beta-cyclodextrin	1520:1545	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	30	theme	beta-cyclodextrin	1529:1545	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	30	theme	beta-cyclodextrin	1529:1545	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	30	theme	beta-cyclodextrin	1529:1545	arg1	g					1466:1466	10 g	1463:1466	10 g of skim milk	1463:1479	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	30	theme	beta-cyclodextrin	1529:1545	arg1	g					1487:1487	8.96 g	1482:1487	8.96 g of resistant-maltodextrin	1482:1513	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	2	31	theme	natural	490:496	arg1	compounds					505:513	the natural active compounds	486:513	the natural active compounds	486:513	Spray-drying increases the bioavailability of the natural active compounds and improves the solubility of low-soluble compounds.
31013876	3	32	theme	material	661:668	arg1	composition					681:691	wall material solution's composition	656:691	wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb	656:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	0	33	theme	Extract	56:62	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.	0:220	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	1	34	theme	compounds	341:349	arg1	protection					307:316	protection	307:316	protection	307:316	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	34	theme	compounds	341:349	arg1	stabilization					289:301	stabilization	289:301	stabilization	289:301	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	9	35	contain	have	1734:1737	arg1	microparticles					1719:1732	the produced microparticles	1706:1732	the produced microparticles	1706:1732	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	9	35	contain	have	1734:1737	arg2	potential					1746:1754	a good potential	1739:1754	a good potential to be included in various pharmaceutical forms or food supplements	1739:1821	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	6	36	theme	sodium	1206:1211	arg1	caseinate					1213:1221	sodium caseinate	1206:1221	sodium caseinate (0.5-1 g)	1206:1231	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	6	36	theme	sodium	1206:1211	arg1	g					1230:1230	0.5-1 g	1224:1230	0.5-1 g	1224:1230	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	3	37	from	composition	681:691	arg1	properties					712:721	physicochemical properties	696:721	physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb	696:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	1	38	theme	popular	247:253	arg1	Spray-drying					222:233	Spray-drying	222:233	Spray-drying	222:233	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	38	theme	popular	247:253	arg1	method					269:274	the most popular encapsulation method	238:274	the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature	238:437	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	7	39	theme	resistant-maltodextrin	1492:1513	arg1	g					1524:1524	0.5 g	1520:1524	0.5 g of beta-cyclodextrin	1520:1545	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	39	theme	resistant-maltodextrin	1492:1513	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	39	theme	resistant-maltodextrin	1492:1513	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	39	theme	resistant-maltodextrin	1492:1513	arg1	g					1466:1466	10 g	1463:1466	10 g of skim milk	1463:1479	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	39	theme	resistant-maltodextrin	1492:1513	arg1	g					1487:1487	8.96 g	1482:1487	8.96 g of resistant-maltodextrin	1482:1513	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	0	40	dep	Microencapsulation	0:17	arg1	Impact					81:86	Impact	81:86	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.	0:220	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	8	41	theme	ciliata	1630:1636	arg1	extracts					1648:1655	E. ciliata ethanolic extracts	1627:1655	E. ciliata ethanolic extracts	1627:1655	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	4	42	theme	suitable	914:921	arg1	materials					928:936	more suitable wall materials	909:936	more suitable wall materials	909:936	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	8	43	theme	extracts	1648:1655	arg1	microencapsulation					1605:1622	the microencapsulation	1601:1622	the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique	1601:1685	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	3	44	theme	microcapsules	726:738	arg1	properties					712:721	physicochemical properties	696:721	physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb	696:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	0	45	theme	Spray-Dried	201:211	arg1	Powders					213:219	Spray-Dried Powders	201:219	Spray-Dried Powders	201:219	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	7	46	theme	sodium	1445:1450	arg1	caseinate					1452:1460	sodium caseinate	1445:1460	sodium caseinate	1445:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	3	47	theme	rich	771:774	arg1	extract					763:769	extract	763:769	extract rich in volatile compounds	763:796	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	1	48	from	protection	307:316	arg1	conditions					378:387	various environmental conditions	356:387	various environmental conditions	356:387	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	7	49	theme	skim	1471:1474	arg1	milk					1476:1479	skim milk	1471:1479	skim milk	1471:1479	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	4	50	theme	dehydroelsholtzia	882:898	arg1	encapsulation					850:862	encapsulation	850:862	encapsulation of elsholtzia and dehydroelsholtzia ketones	850:906	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	1	51	from	stabilization	289:301	arg1	conditions					378:387	various environmental conditions	356:387	various environmental conditions	356:387	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	9	52	theme	produced	1710:1717	arg1	microparticles					1719:1732	the produced microparticles	1706:1732	the produced microparticles	1706:1732	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	7	53	theme	caseinate	1452:1460	arg1	g					1524:1524	0.5 g	1520:1524	0.5 g of beta-cyclodextrin	1520:1545	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	53	theme	caseinate	1452:1460	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	53	theme	caseinate	1452:1460	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	53	theme	caseinate	1452:1460	arg1	g					1466:1466	10 g	1463:1466	10 g of skim milk	1463:1479	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	53	theme	caseinate	1452:1460	arg1	g					1487:1487	8.96 g	1482:1487	8.96 g of resistant-maltodextrin	1482:1513	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	6	54	theme	physicochemical	1371:1385	arg1	properties					1387:1396	physicochemical properties	1371:1396	physicochemical properties	1371:1396	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	3	55	theme	work	585:588	arg1	aim					573:575	The aim	569:575	The aim of this work	569:588	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	3	56	theme	volatile	779:786	arg1	compounds					788:796	volatile compounds	779:796	volatile compounds	779:796	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	9	57	theme	good	1741:1744	arg1	potential					1746:1754	a good potential	1739:1754	a good potential to be included in various pharmaceutical forms or food supplements	1739:1821	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	3	58	theme	Elsholtzia	821:830	arg1	herb					840:843	Elsholtzia ciliata herb	821:843	Elsholtzia ciliata herb	821:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	6	59	theme	drying	1353:1358	arg1	yield					1360:1364	drying yield	1353:1364	drying yield	1353:1364	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	5	60	theme	phenolics-sodium	993:1008	arg1	caseinate					1010:1018	Four phenolics-sodium caseinate	988:1018	Four phenolics-sodium caseinate	988:1018	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	4	61	theme	sodium	970:975	arg1	caseinate					977:985	sodium caseinate	970:985	sodium caseinate	970:985	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	7	62	theme	optimal	1403:1409	arg1	composition					1419:1429	The optimal mixture composition	1399:1429	The optimal mixture composition	1399:1429	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	7	62	theme	optimal	1403:1409	arg1	g					1440:1440	0.54 g	1435:1440	0.54 g of sodium caseinate	1435:1460	The optimal mixture composition was 0.54 g of sodium caseinate, 10 g of skim milk, 8.96 g of resistant-maltodextrin, and 0.5 g of beta-cyclodextrin.
31013876	3	63	theme	wall	628:631	arg1	materials					633:641	different wall materials	618:641	different wall materials	618:641	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	6	64	theme	composition	1109:1119	arg1	design					1121:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	9	65	theme	various	1774:1780	arg1	forms					1797:1801	various pharmaceutical forms	1774:1801	various pharmaceutical forms	1774:1801	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	2	66	theme	compounds	558:566	arg1	solubility					532:541	the solubility	528:541	the solubility of low-soluble compounds	528:566	Spray-drying increases the bioavailability of the natural active compounds and improves the solubility of low-soluble compounds.
31013876	1	67	theme	environmental	364:376	arg1	conditions					378:387	various environmental conditions	356:387	various environmental conditions	356:387	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	6	68	theme	D-optimal	1091:1099	arg1	mixture					1101:1107	A D-optimal mixture	1089:1107	A D-optimal mixture composition design	1089:1126	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	1	69	dep	stabilization	289:301	arg1	the					285:287	the	285:287	the	285:287	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	9	70	theme	food	1806:1809	arg1	supplements					1811:1821	food supplements	1806:1821	food supplements	1806:1821	It is proven that the produced microparticles have a good potential to be included in various pharmaceutical forms or food supplements.
31013876	0	71	theme	Sodium	132:137	arg1	Caseinate					139:147	Sodium Caseinate	132:147	Sodium Caseinate	132:147	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	6	72	theme	material	1168:1175	arg1	effect					1153:1158	the effect	1149:1158	the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties	1149:1396	A D-optimal mixture composition design was used to evaluate the effect of wall material solution's composition using sodium caseinate (0.5-1 g), skim milk (6-10 g), resistant-maltodextrin (8-12 g), and beta-cyclodextrin (0.5-1 g) for the encapsulation efficiency, drying yield, and physicochemical properties.
31013876	8	73	theme	encapsulating	1554:1566	arg1	agents					1568:1573	These encapsulating agents	1548:1573	These encapsulating agents	1548:1573	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	0	74	theme	ciliata	33:39	arg1	Extract					56:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	8	75	theme	good	1581:1584	arg1	performance					1586:1596	a good performance	1579:1596	a good performance	1579:1596	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	0	76	theme	Ethanolic	46:54	arg1	Extract					56:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Elsholtzia ciliata Herb Ethanolic Extract	22:62	Microencapsulation of Elsholtzia ciliata Herb Ethanolic Extract by Spray-Drying: Impact of Resistant-Maltodextrin Complemented with Sodium Caseinate, Skim Milk, and Beta-Cyclodextrin on the Quality of Spray-Dried Powders.
31013876	2	77	theme	active	498:503	arg1	compounds					505:513	the natural active compounds	486:513	the natural active compounds	486:513	Spray-drying increases the bioavailability of the natural active compounds and improves the solubility of low-soluble compounds.
31013876	3	78	theme	wall	656:659	arg1	material					661:668	wall material	656:668	wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb	656:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	1	79	theme	active	334:339	arg1	pH					419:420	pH	419:420	pH	419:420	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	79	theme	active	334:339	arg1	temperature					427:437	temperature	427:437	temperature	427:437	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	79	theme	active	334:339	arg1	moisture					409:416	moisture	409:416	moisture	409:416	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	79	theme	active	334:339	arg1	oxidation					398:406	oxidation	398:406	oxidation	398:406	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	79	theme	active	334:339	arg1	compounds					341:349	biologically active compounds	321:349	biologically active compounds	321:349	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	3	80	from	herb	840:843	arg1	oil					812:814	essential oil	802:814	essential oil from Elsholtzia ciliata herb	802:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	3	80	from	herb	840:843	arg1	extract					763:769	extract	763:769	extract rich in volatile compounds	763:796	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	3	80	from	herb	840:843	arg1	phenolics					752:760	phenolics	752:760	phenolics	752:760	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	3	81	theme	physicochemical	696:710	arg1	properties					712:721	physicochemical properties	696:721	physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb	696:843	The aim of this work was to study the effects of different wall materials and optimize wall material solution's composition on physicochemical properties of microcapsules loaded with phenolics, extract rich in volatile compounds and essential oil from Elsholtzia ciliata herb.
31013876	4	82	theme	elsholtzia	867:876	arg1	encapsulation					850:862	encapsulation	850:862	encapsulation of elsholtzia and dehydroelsholtzia ketones	850:906	For encapsulation of elsholtzia and dehydroelsholtzia ketones, more suitable wall materials were used-beta-cyclodextrin and sodium caseinate.
31013876	8	83	theme	E.	1627:1628	arg1	extracts					1648:1655	E. ciliata ethanolic extracts	1627:1655	E. ciliata ethanolic extracts	1627:1655	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	5	84	used	used	1083:1086	arg2	caseinate					1010:1018	Four phenolics-sodium caseinate	988:1018	Four phenolics-sodium caseinate	988:1018	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	5	84	used	used	1083:1086	arg2	resistant-maltodextrin-were					1055:1081	resistant-maltodextrin-were	1055:1081	resistant-maltodextrin-were	1055:1081	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	5	84	used	used	1083:1086	arg2	beta-cyclodextrin					1032:1048	beta-cyclodextrin	1032:1048	beta-cyclodextrin	1032:1048	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	5	84	used	used	1083:1086	arg2	milk					1026:1029	skim milk	1021:1029	skim milk	1021:1029	Four phenolics-sodium caseinate, skim milk, beta-cyclodextrin, and resistant-maltodextrin-were used.
31013876	8	85	theme	ethanolic	1638:1646	arg1	extracts					1648:1655	E. ciliata ethanolic extracts	1627:1655	E. ciliata ethanolic extracts	1627:1655	These encapsulating agents had a good performance in the microencapsulation of E. ciliata ethanolic extracts by the spray-drying technique.
31013876	1	86	theme	encapsulation	255:267	arg1	Spray-drying					222:233	Spray-drying	222:233	Spray-drying	222:233	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31013876	1	86	theme	encapsulation	255:267	arg1	method					269:274	the most popular encapsulation method	238:274	the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature	238:437	Spray-drying is the most popular encapsulation method used for the stabilization and protection of biologically active compounds from various environmental conditions, such as oxidation, moisture, pH, and temperature.
31868924	0	0	theme	biomass	91:97	arg1	particles					99:107	individual biomass particles	80:107	individual biomass particles	80:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	4	1	theme	isolated	788:795	arg1	blocks					806:811	isolated building blocks	788:811	isolated building blocks of plant cell wall	788:830	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	2	2	theme	recalcitrance	398:410	arg1	results					412:418	variable recalcitrance results	389:418	variable recalcitrance results	389:418	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	5	3	from	decrease	1063:1070	arg1	cellulose					997:1005	cellulose	997:1005	cellulose	997:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	0	4	theme	individual	80:89	arg1	particles					99:107	individual biomass particles	80:107	individual biomass particles	80:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	5	5	theme	second	969:974	arg1	signal					985:990	second harmonic signal	969:990	second harmonic signal from cellulose	969:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	2	6	theme	variable	389:396	arg1	results					412:418	variable recalcitrance results	389:418	variable recalcitrance results	389:418	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	7	7	theme	enzymatic	1308:1316	arg1	hydrolysis					1318:1327	analyzing enzymatic hydrolysis	1298:1327	analyzing enzymatic hydrolysis in vitro and in situ	1298:1348	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	7	8	dep	cocktails	1393:1401	arg1	targeting					1403:1411	targeting	1403:1411	targeting specific recalcitrant structures	1403:1444	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	7	8	dep	cocktails	1393:1401	arg1	investigating					1449:1461	investigating	1449:1461	investigating locally enzyme anti-inhibitory agents	1449:1499	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	3	9	theme	stover	642:647	arg1	particles					649:657	individual corn stover particles	626:657	individual corn stover particles pretreated with sulfuric acid during hydrolysis	626:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	7	10	theme	specific	1413:1420	arg1	structures					1435:1444	specific recalcitrant structures	1413:1444	specific recalcitrant structures	1413:1444	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	5	11	theme	emission	1040:1047	arg1	reduction					926:934	particle size reduction	912:934	particle size reduction	912:934	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	11	theme	emission	1040:1047	arg1	decrease					957:964	decrease	957:964	decrease of second harmonic signal from cellulose	957:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	11	theme	emission	1040:1047	arg1	collapse					947:954	side wall collapse	937:954	side wall collapse	937:954	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	11	theme	emission	1040:1047	arg1	redshifting					1008:1018	redshifting	1008:1018	redshifting of autofluorescence emission	1008:1047	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	11	theme	emission	1040:1047	arg1	decrease					1063:1070	lifetime decrease	1054:1070	lifetime decrease attributed to the relative increase of lignin	1054:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	0	12	theme	particles	99:107	arg1	hydrolysis					66:75	in situ enzymatic hydrolysis	48:75	in situ enzymatic hydrolysis of individual biomass particles	48:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	5	13	from	decrease	957:964	arg1	cellulose					997:1005	cellulose	997:1005	cellulose	997:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	14	theme	chemical	606:613	arg1	changes					615:621	morphological and chemical changes	588:621	morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis	588:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	15	theme	side	937:940	arg1	collapse					947:954	side wall collapse	937:954	side wall collapse	937:954	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	0	16	theme	autofluorescence	118:133	arg1	imaging					135:141	autofluorescence imaging	118:141	autofluorescence imaging	118:141	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	4	17	theme	blocks	806:811	arg1	lignin					871:876	lignin	871:876	lignin	871:876	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	17	theme	blocks	806:811	arg1	cellulose					841:849	cellulose	841:849	cellulose	841:849	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	17	theme	blocks	806:811	arg1	properties					774:783	the fluorescence properties	757:783	the fluorescence properties	757:783	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	17	theme	blocks	806:811	arg1	hemicellulose					852:864	hemicellulose	852:864	hemicellulose	852:864	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	18	theme	wall	827:830	arg1	blocks					806:811	isolated building blocks	788:811	isolated building blocks of plant cell wall	788:830	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	2	19	theme	big	305:307	arg1	variation					309:317	big variation	305:317	big variation on conversion rates	305:337	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	6	20	theme	tracking	1148:1155	arg1	change					1171:1176	tracking compositional change	1148:1176	tracking compositional change after hydrolysis of individual particles	1148:1217	Based on these observations, tracking compositional change after hydrolysis of individual particles was accomplished.
31868924	1	21	theme	established	182:192	arg1	hydrolysis					154:163	Enzymatic hydrolysis	144:163	Enzymatic hydrolysis of biomass	144:174	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31868924	1	21	theme	established	182:192	arg1	method					194:199	an established method	179:199	an established method for producing biofuels	179:222	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31868924	7	22	theme	analyzing	1298:1306	arg1	hydrolysis					1318:1327	analyzing enzymatic hydrolysis	1298:1327	analyzing enzymatic hydrolysis in vitro and in situ	1298:1348	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	5	23	theme	harmonic	976:983	arg1	signal					985:990	second harmonic signal	969:990	second harmonic signal from cellulose	969:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	24	theme	noninvasive	444:454	arg1	techniques					475:484	noninvasive optical microscopy techniques	444:484	noninvasive optical microscopy techniques	444:484	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	25	from	cellulose	997:1005	arg1	reduction					926:934	particle size reduction	912:934	particle size reduction	912:934	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	25	from	cellulose	997:1005	arg1	decrease					957:964	decrease	957:964	decrease of second harmonic signal from cellulose	957:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	25	from	cellulose	997:1005	arg1	decrease					1063:1070	lifetime decrease	1054:1070	lifetime decrease attributed to the relative increase of lignin	1054:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	25	from	cellulose	997:1005	arg1	redshifting					1008:1018	redshifting	1008:1018	redshifting of autofluorescence emission	1008:1047	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	25	from	cellulose	997:1005	arg1	collapse					947:954	side wall collapse	937:954	side wall collapse	937:954	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	25	from	cellulose	997:1005	arg1	signal					985:990	second harmonic signal	969:990	second harmonic signal from cellulose	969:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	26	theme	morphological	588:600	arg1	changes					615:621	morphological and chemical changes	588:621	morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis	588:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	27	theme	wall	942:945	arg1	collapse					947:954	side wall collapse	937:954	side wall collapse	937:954	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	28	theme	Enzymatic	879:887	arg1	hydrolysis					889:898	Enzymatic hydrolysis	879:898	Enzymatic hydrolysis	879:898	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	2	29	theme	individual	347:356	arg1	particles					358:366	individual particles	347:366	individual particles	347:366	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	3	30	theme	microscopy	464:473	arg1	techniques					475:484	noninvasive optical microscopy techniques	444:484	noninvasive optical microscopy techniques	444:484	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	3	31	theme	corn	637:640	arg1	particles					649:657	individual corn stover particles	626:657	individual corn stover particles pretreated with sulfuric acid during hydrolysis	626:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	0	32	theme	changes	33:39	arg1	Quantification					0:13	Quantification	0:13	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles	0:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	4	33	theme	cell	822:825	arg1	wall					827:830	plant cell wall	816:830	plant cell wall	816:830	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	3	34	dep	such	487:490	arg1	as					492:493	as	492:493	as	492:493	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	35	theme	autofluorescence	1023:1038	arg1	emission					1040:1047	autofluorescence emission	1023:1047	autofluorescence emission	1023:1047	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	6	36	theme	compositional	1157:1169	arg1	change					1171:1176	tracking compositional change	1148:1176	tracking compositional change after hydrolysis of individual particles	1148:1217	Based on these observations, tracking compositional change after hydrolysis of individual particles was accomplished.
31868924	4	37	theme	Morphochemical	708:721	arg1	changes					723:729	Morphochemical changes	708:729	Morphochemical changes	708:729	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	0	38	theme	morphochemical	18:31	arg1	changes					33:39	morphochemical changes	18:39	morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles	18:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	5	39	theme	lifetime	1054:1061	arg1	decrease					1063:1070	lifetime decrease	1054:1070	lifetime decrease attributed to the relative increase of lignin	1054:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	2	40	theme	conversion	322:331	arg1	rates					333:337	conversion rates	322:337	conversion rates	322:337	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	5	41	theme	lignin	1111:1116	arg1	increase					1099:1106	the relative increase	1086:1106	the relative increase of lignin	1086:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	42	theme	size	921:924	arg1	reduction					926:934	particle size reduction	912:934	particle size reduction	912:934	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	0	43	theme	in	48:49	arg1	hydrolysis					66:75	in situ enzymatic hydrolysis	48:75	in situ enzymatic hydrolysis of individual biomass particles	48:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	4	44	theme	building	797:804	arg1	blocks					806:811	isolated building blocks	788:811	isolated building blocks of plant cell wall	788:830	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	7	45	used	used	1366:1369	arg2	paradigm					1273:1280	a paradigm	1271:1280	a paradigm	1271:1280	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	7	46	theme	enzymes	1385:1391	arg1	cocktails					1393:1401	enzymes cocktails	1385:1401	enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents	1385:1499	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	3	47	theme	optical	456:462	arg1	techniques					475:484	noninvasive optical microscopy techniques	444:484	noninvasive optical microscopy techniques	444:484	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	7	48	theme	recalcitrant	1422:1433	arg1	structures					1435:1444	specific recalcitrant structures	1413:1444	specific recalcitrant structures	1413:1444	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	2	49	theme	corn	257:260	arg1	stover					262:267	corn stover	257:267	corn stover	257:267	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	5	50	from	reduction	926:934	arg1	cellulose					997:1005	cellulose	997:1005	cellulose	997:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	51	theme	two-photon	495:504	arg1	microscopy					506:515	two-photon microscopy	495:515	two-photon microscopy	495:515	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	0	52	theme	enzymatic	56:64	arg1	hydrolysis					66:75	in situ enzymatic hydrolysis	48:75	in situ enzymatic hydrolysis of individual biomass particles	48:107	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	3	53	used	used	439:442	arg2	we					436:437	we	436:437	we	436:437	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	0	54	dep	in	48:49	arg1	situ					51:54	situ	51:54	situ	51:54	Quantification of morphochemical changes during in situ enzymatic hydrolysis of individual biomass particles based on autofluorescence imaging.
31868924	3	55	theme	imaging	543:549	arg1	microscopy					551:560	fluorescence lifetime imaging microscopy	521:560	fluorescence lifetime imaging microscopy	521:560	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	2	56	with	material	291:298	arg1	variation					309:317	big variation	305:317	big variation on conversion rates	305:337	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	3	57	theme	particles	649:657	arg1	changes					615:621	morphological and chemical changes	588:621	morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis	588:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	4	58	theme	plant	816:820	arg1	wall					827:830	plant cell wall	816:830	plant cell wall	816:830	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	3	59	theme	individual	626:635	arg1	particles					649:657	individual corn stover particles	626:657	individual corn stover particles pretreated with sulfuric acid during hydrolysis	626:705	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	2	60	theme	Lignocellulosic	225:239	arg1	biomass					241:247	Lignocellulosic biomass	225:247	Lignocellulosic biomass such as corn stover	225:267	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	7	61	theme	enzyme	1471:1476	arg1	agents					1494:1499	locally enzyme anti-inhibitory agents	1463:1499	locally enzyme anti-inhibitory agents	1463:1499	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	4	62	theme	fluorescence	761:772	arg1	lignin					871:876	lignin	871:876	lignin	871:876	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	62	theme	fluorescence	761:772	arg1	cellulose					841:849	cellulose	841:849	cellulose	841:849	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	62	theme	fluorescence	761:772	arg1	properties					774:783	the fluorescence properties	757:783	the fluorescence properties	757:783	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	4	62	theme	fluorescence	761:772	arg1	hemicellulose					852:864	hemicellulose	852:864	hemicellulose	852:864	Morphochemical changes were interpreted based on the fluorescence properties of isolated building blocks of plant cell wall, such as cellulose, hemicellulose, and lignin.
31868924	1	63	theme	Enzymatic	144:152	arg1	hydrolysis					154:163	Enzymatic hydrolysis	144:163	Enzymatic hydrolysis of biomass	144:174	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31868924	1	63	theme	Enzymatic	144:152	arg1	method					194:199	an established method	179:199	an established method for producing biofuels	179:222	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31868924	6	64	theme	particles	1209:1217	arg1	hydrolysis					1184:1193	hydrolysis	1184:1193	hydrolysis of individual particles	1184:1217	Based on these observations, tracking compositional change after hydrolysis of individual particles was accomplished.
31868924	5	65	theme	signal	985:990	arg1	reduction					926:934	particle size reduction	912:934	particle size reduction	912:934	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	65	theme	signal	985:990	arg1	decrease					957:964	decrease	957:964	decrease of second harmonic signal from cellulose	957:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	65	theme	signal	985:990	arg1	collapse					947:954	side wall collapse	937:954	side wall collapse	937:954	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	65	theme	signal	985:990	arg1	redshifting					1008:1018	redshifting	1008:1018	redshifting of autofluorescence emission	1008:1047	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	5	65	theme	signal	985:990	arg1	decrease					1063:1070	lifetime decrease	1054:1070	lifetime decrease attributed to the relative increase of lignin	1054:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	2	66	theme	inhomogeneous	277:289	arg1	material					291:298	inhomogeneous material	277:298	inhomogeneous material with big variation on conversion rates between individual particles	277:366	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	5	67	from	collapse	947:954	arg1	cellulose					997:1005	cellulose	997:1005	cellulose	997:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	68	theme	lifetime	534:541	arg1	microscopy					551:560	fluorescence lifetime imaging microscopy	521:560	fluorescence lifetime imaging microscopy	521:560	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	69	from	redshifting	1008:1018	arg1	cellulose					997:1005	cellulose	997:1005	cellulose	997:1005	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	3	70	theme	fluorescence	521:532	arg1	microscopy					551:560	fluorescence lifetime imaging microscopy	521:560	fluorescence lifetime imaging microscopy	521:560	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	3	71	theme	sulfuric	675:682	arg1	acid					684:687	sulfuric acid	675:687	sulfuric acid	675:687	In this study, we used noninvasive optical microscopy techniques, such as two-photon microscopy and fluorescence lifetime imaging microscopy, to visualize and analyze morphological and chemical changes of individual corn stover particles pretreated with sulfuric acid during hydrolysis.
31868924	5	72	theme	particle	912:919	arg1	reduction					926:934	particle size reduction	912:934	particle size reduction	912:934	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	6	73	theme	individual	1198:1207	arg1	particles					1209:1217	individual particles	1198:1217	individual particles	1198:1217	Based on these observations, tracking compositional change after hydrolysis of individual particles was accomplished.
31868924	2	74	from	variation	309:317	arg1	rates					333:337	conversion rates	322:337	conversion rates	322:337	Lignocellulosic biomass such as corn stover is very inhomogeneous material with big variation on conversion rates between individual particles therefore leading to variable recalcitrance results.
31868924	7	75	theme	anti-inhibitory	1478:1492	arg1	agents					1494:1499	locally enzyme anti-inhibitory agents	1463:1499	locally enzyme anti-inhibitory agents	1463:1499	The methodologies developed offer a paradigm for imaging and analyzing enzymatic hydrolysis in vitro and in situ, which could be used for screening enzymes cocktails targeting specific recalcitrant structures or investigating locally enzyme anti-inhibitory agents.
31868924	5	76	theme	relative	1090:1097	arg1	increase					1099:1106	the relative increase	1086:1106	the relative increase of lignin	1086:1116	Enzymatic hydrolysis resulted in particle size reduction, side wall collapse, decrease of second harmonic signal from cellulose, redshifting of autofluorescence emission, and lifetime decrease attributed to the relative increase of lignin.
31868924	1	77	theme	biomass	168:174	arg1	hydrolysis					154:163	Enzymatic hydrolysis	144:163	Enzymatic hydrolysis of biomass	144:174	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31868924	1	77	theme	biomass	168:174	arg1	method					194:199	an established method	179:199	an established method for producing biofuels	179:222	Enzymatic hydrolysis of biomass is an established method for producing biofuels.
31372513	0	0	theme	liver	95:99	arg1	risk					108:111	liver injury risk	95:111	liver injury risk	95:111	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	5	1	theme	LGG	691:693	arg1	EPS					695:697	LGG EPS	691:697	LGG EPS	691:697	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	3	2	theme	high-fat	475:482	arg1	diet					484:487	a high-fat diet	473:487	a high-fat diet	473:487	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	6	3	theme	injury	974:979	arg1	risk					981:984	liver injury risk	968:984	liver injury risk	968:984	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	5	4	theme	peptide	805:811	arg1	expression					813:822	local antimicrobial peptide expression	785:822	local antimicrobial peptide expression	785:822	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	6	5	theme	Lactobacillus-derived	1005:1025	arg1	EPS					1027:1029	Lactobacillus-derived EPS	1005:1029	Lactobacillus-derived EPS	1005:1029	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	5	6	theme	BL23	914:917	arg1	microbiota					934:943	BL23 EPS-associated microbiota	914:943	BL23 EPS-associated microbiota	914:943	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	5	7	theme	antimicrobial	791:803	arg1	expression					813:822	local antimicrobial peptide expression	785:822	local antimicrobial peptide expression	785:822	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	5	8	theme	distinct	876:883	arg1	compositions					885:896	the distinct compositions	872:896	the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota	872:943	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	0	9	theme	injury	101:106	arg1	risk					108:111	liver injury risk	95:111	liver injury risk	95:111	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	6	10	theme	used	1070:1073	arg1	polysaccharides					1083:1097	commonly used natural polysaccharides	1061:1097	commonly used natural polysaccharides	1061:1097	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	3	11	dep	Lactobacillus	380:392	arg1	GG					404:405	Lactobacillus rhamnosus GG	380:405	Lactobacillus rhamnosus GG (LGG)	380:411	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	3	11	dep	Lactobacillus	380:392	arg1	BL23					426:429	casei BL23	420:429	casei BL23	420:429	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	6	12	theme	activation	1125:1134	arg1	efficiency					1136:1145	their HIF1α activation efficiency	1113:1145	their HIF1α activation efficiency	1113:1145	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	2	13	theme	use	294:296	arg1	risks					279:283	the potential risks	265:283	the potential risks of their use	265:296	However, the potential risks of their use have been less investigated.
31372513	6	14	theme	liver	968:972	arg1	risk					981:984	liver injury risk	968:984	liver injury risk	968:984	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	3	15	theme	BL23	496:499	arg1	EPS					501:503	BL23 EPS	496:503	BL23 EPS	496:503	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	4	16	theme	BL23	590:593	arg1	EPS					595:597	BL23 EPS	590:597	BL23 EPS	590:597	This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis.
31372513	5	17	theme	EPS-	905:908	arg1	compositions					885:896	the distinct compositions	872:896	the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota	872:943	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	3	18	theme	casei	420:424	arg1	BL23					426:429	casei BL23	420:429	casei BL23	420:429	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	3	19	from	Lactobacillus	380:392	arg1	EPS					370:372	EPS	370:372	EPS	370:372	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	3	19	from	Lactobacillus	380:392	arg1	exopolysaccharides					350:367	the exopolysaccharides	346:367	the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23	346:429	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	6	20	theme	natural	1075:1081	arg1	polysaccharides					1083:1097	commonly used natural polysaccharides	1061:1097	commonly used natural polysaccharides	1061:1097	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	4	21	theme	microbial	655:663	arg1	homeostasis					665:675	microbial homeostasis	655:675	microbial homeostasis	655:675	This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis.
31372513	0	22	theme	polysaccharides	21:35	arg1	Ability					0:6	Ability	0:6	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis	0:82	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	6	23	theme	HIF1α	1119:1123	arg1	efficiency					1136:1145	their HIF1α activation efficiency	1113:1145	their HIF1α activation efficiency	1113:1145	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	3	24	theme	hepatic	438:444	arg1	steatosis					446:454	hepatic steatosis	438:454	hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury	438:558	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	0	25	theme	prebiotic	11:19	arg1	polysaccharides					21:35	prebiotic polysaccharides	11:35	prebiotic polysaccharides	11:35	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	5	26	theme	LGG	901:903	arg1	EPS-					905:908	LGG EPS-	901:908	LGG EPS-	901:908	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	3	27	theme	LGG	514:516	arg1	EPS					518:520	LGG EPS	514:520	LGG EPS	514:520	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	5	28	theme	intestinal	740:749	arg1	HIF1α					751:755	intestinal HIF1α	740:755	intestinal HIF1α	740:755	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	6	29	theme	other	1046:1050	arg1	types					1052:1056	other types	1046:1056	other types of commonly used natural polysaccharides	1046:1097	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	0	30	theme	HIF1α-antimicrobial	51:69	arg1	axis					79:82	a HIF1α-antimicrobial peptide axis	49:82	a HIF1α-antimicrobial peptide axis	49:82	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	5	31	theme	local	785:789	arg1	expression					813:822	local antimicrobial peptide expression	785:822	local antimicrobial peptide expression	785:822	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	4	32	theme	LGG	638:640	arg1	EPS					642:644	LGG EPS	638:644	LGG EPS	638:644	This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis.
31372513	5	33	theme	BL23	708:711	arg1	EPS					713:715	BL23 EPS	708:715	BL23 EPS	708:715	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	6	34	link	Lactobacillus-derived	1005:1025	arg1	EPS					1027:1029	Lactobacillus-derived EPS	1005:1029	Lactobacillus-derived EPS	1005:1029	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	3	35	theme	liver	530:534	arg1	inflammation					536:547	liver inflammation	530:547	liver inflammation	530:547	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	1	36	theme	Natural	127:133	arg1	polysaccharides					135:149	Natural polysaccharides	127:149	Natural polysaccharides	127:149	Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet.
31372513	3	37	from	steatosis	446:454	arg1	zebrafish					459:467	zebrafish	459:467	zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury	459:558	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	5	38	theme	microbial	838:846	arg1	homeostasis					848:858	microbial homeostasis	838:858	microbial homeostasis	838:858	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	1	39	theme	high-fat	241:248	arg1	diet					250:253	high-fat diet	241:253	high-fat diet	241:253	Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet.
31372513	6	40	theme	polysaccharides	1083:1097	arg1	types					1052:1056	other types	1046:1056	other types of commonly used natural polysaccharides	1046:1097	Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
31372513	2	41	theme	potential	269:277	arg1	risks					279:283	the potential risks	265:283	the potential risks of their use	265:296	However, the potential risks of their use have been less investigated.
31372513	4	42	theme	gut	607:609	arg1	dysbiosis					621:629	gut microbial dysbiosis	607:629	gut microbial dysbiosis	607:629	This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis.
31372513	5	43	theme	EPS-associated	919:932	arg1	microbiota					934:943	BL23 EPS-associated microbiota	914:943	BL23 EPS-associated microbiota	914:943	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	1	44	theme	hepatic	212:218	arg1	steatosis					220:228	hepatic steatosis	212:228	hepatic steatosis induced by high-fat diet	212:253	Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet.
31372513	4	45	theme	microbial	611:619	arg1	dysbiosis					621:629	gut microbial dysbiosis	607:629	gut microbial dysbiosis	607:629	This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis.
31372513	5	46	theme	increased	762:770	arg1	HIF1α					772:776	increased HIF1α	762:776	increased HIF1α	762:776	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	0	47	theme	peptide	71:77	arg1	axis					79:82	a HIF1α-antimicrobial peptide axis	49:82	a HIF1α-antimicrobial peptide axis	49:82	Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish.
31372513	5	48	theme	microbiota	934:943	arg1	compositions					885:896	the distinct compositions	872:896	the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota	872:943	We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota.
31372513	3	49	from	L.	417:418	arg1	EPS					370:372	EPS	370:372	EPS	370:372	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	3	49	from	L.	417:418	arg1	exopolysaccharides					350:367	the exopolysaccharides	346:367	the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23	346:429	Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury.
31372513	1	50	theme	much	165:168	arg1	attention					170:178	much attention	165:178	much attention	165:178	Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet.
31382336	0	0	theme	stem	86:89	arg1	cells					91:95	bone marrow stem cells	74:95	bone marrow stem cells delivery carrier	74:112	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	1	1	theme	network	139:145	arg1	hydrogel					147:154	a double network hydrogel	130:154	a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel	130:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	2	theme	synthetic	215:223	arg1	hydrogel					258:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel	213:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH)	213:278	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	2	theme	synthetic	215:223	arg1	DNH					275:277	DNH	275:277	DNH	275:277	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	3	theme	marrow	79:84	arg1	cells					91:95	bone marrow stem cells	74:95	bone marrow stem cells delivery carrier	74:112	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	3	4	theme	stem	655:658	arg1	cells					660:664	bone marrow stem cells	643:664	bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro	643:726	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	5	5	theme	cell	848:851	arg1	distribution					853:864	cell distribution	848:864	cell distribution	848:864	Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo.
31382336	1	6	theme	hydrogel	225:232	arg1	hydrogel					258:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel	213:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH)	213:278	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	6	theme	hydrogel	225:232	arg1	DNH					275:277	DNH	275:277	DNH	275:277	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	7	theme	delivery	97:104	arg1	carrier					106:112	bone marrow stem cells delivery carrier	74:112	bone marrow stem cells delivery carrier	74:112	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	3	8	theme	cells	660:664	arg1	encapsulation					626:638	encapsulation	626:638	encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro	626:726	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	6	9	from	hydrogels	1006:1014	arg1	purpose					1038:1044	tissue engineering purpose	1019:1044	tissue engineering purpose	1019:1044	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	1	10	theme	poloxamer-heparin	234:250	arg1	hydrogel					258:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel	213:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH)	213:278	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	10	theme	poloxamer-heparin	234:250	arg1	DNH					275:277	DNH	275:277	DNH	275:277	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	11	theme	cells	91:95	arg1	carrier					106:112	bone marrow stem cells delivery carrier	74:112	bone marrow stem cells delivery carrier	74:112	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	5	12	theme	PoH/GG	915:920	arg1	DNH					922:924	PoH/GG DNH	915:924	PoH/GG DNH	915:924	Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo.
31382336	2	13	theme	sol	479:481	arg1	fraction					483:490	sol fraction	479:490	sol fraction	479:490	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	6	14	theme	engineering	1026:1036	arg1	purpose					1038:1044	tissue engineering purpose	1019:1044	tissue engineering purpose	1019:1044	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	1	15	theme	hydrogel	330:337	arg1	disadvantages					308:320	disadvantages	308:320	disadvantages of each hydrogel	308:337	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	6	16	theme	potential	961:969	arg1	opportunity					971:981	a potential opportunity	959:981	a potential opportunity to apply the composite hydrogels in tissue engineering purpose	959:1044	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	6	17	theme	tissue	1019:1024	arg1	engineering					1026:1036	tissue engineering	1019:1036	tissue engineering purpose	1019:1044	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	4	18	theme	rBMSCs	805:810	arg1	morphology					812:821	rBMSCs morphology	805:821	rBMSCs morphology	805:821	The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype.
31382336	3	19	theme	PoH/GG	708:713	arg1	DNH					715:717	the PoH/GG DNH	704:717	the PoH/GG DNH	704:717	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	5	20	theme	ECM	882:884	arg1	production					886:895	ECM production	882:895	ECM production	882:895	Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo.
31382336	1	21	theme	natural	161:167	arg1	hydrogel					200:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	2	22	theme	surface	411:417	arg1	morphology					419:428	surface morphology	411:428	surface morphology	411:428	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	1	23	theme	PoH	253:255	arg1	hydrogel					258:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel	213:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH)	213:278	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	23	theme	PoH	253:255	arg1	DNH					275:277	DNH	275:277	DNH	275:277	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	3	24	theme	bone	643:646	arg1	marrow					648:653	bone marrow	643:653	bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro	643:726	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	1	25	theme	polysaccharide	169:182	arg1	hydrogel					200:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	26	theme	network	21:27	arg1	hydrogel					29:36	double network hydrogel	14:36	double network hydrogel of poloxamer-heparin/gellan gum	14:68	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	2	27	theme	thermal	517:523	arg1	stability					525:533	thermal stability	517:533	thermal stability	517:533	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	1	28	theme	gellan	184:189	arg1	hydrogel					200:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	29	theme	double	14:19	arg1	hydrogel					29:36	double network hydrogel	14:36	double network hydrogel of poloxamer-heparin/gellan gum	14:68	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	2	30	theme	gelation	431:438	arg1	temperature					440:450	gelation temperature	431:450	gelation temperature	431:450	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	1	31	theme	gum	191:193	arg1	hydrogel					200:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	32	theme	hydrogel	29:36	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.	0:113	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	2	33	theme	mechanical	493:502	arg1	property					504:511	mechanical property	493:511	mechanical property	493:511	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	6	34	theme	Overall	935:941	arg1	results					943:949	Overall results	935:949	Overall results	935:949	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	4	35	theme	DNH	757:759	arg1	system					761:766	the DNH system	753:766	the DNH system	753:766	The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype.
31382336	1	36	theme	GG	196:197	arg1	hydrogel					200:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	a natural polysaccharide gellan gum (GG) hydrogel	159:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	37	theme	gum	66:68	arg1	hydrogel					29:36	double network hydrogel	14:36	double network hydrogel of poloxamer-heparin/gellan gum	14:68	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	4	38	theme	cell	778:781	arg1	survival					783:790	cell survival	778:790	cell survival	778:790	The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype.
31382336	0	39	theme	poloxamer-heparin/gellan	41:64	arg1	gum					66:68	poloxamer-heparin/gellan gum	41:68	poloxamer-heparin/gellan gum	41:68	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	1	40	theme	cell	376:379	arg1	delivery					381:388	cell delivery	376:388	cell delivery	376:388	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	3	41	theme	marrow	648:653	arg1	cells					660:664	bone marrow stem cells	643:664	bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro	643:726	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	3	42	theme	cell	593:596	arg1	vehicle					598:604	cell vehicle	593:604	cell vehicle	593:604	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	1	43	theme	hydrogel	200:207	arg1	hydrogel					258:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel	213:265	a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH)	213:278	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	43	theme	hydrogel	200:207	arg1	DNH					275:277	DNH	275:277	DNH	275:277	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	1	43	theme	hydrogel	200:207	arg1	hydrogel					147:154	a double network hydrogel	130:154	a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel	130:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	0	44	theme	bone	74:77	arg1	cells					91:95	bone marrow stem cells	74:95	bone marrow stem cells delivery carrier	74:112	Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier.
31382336	3	45	theme	composite	570:578	arg1	hydrogel					580:587	the composite hydrogel	566:587	the composite hydrogel for cell vehicle	566:604	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	6	46	theme	composite	996:1004	arg1	hydrogels					1006:1014	the composite hydrogels	992:1014	the composite hydrogels in tissue engineering purpose	992:1044	Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.
31382336	2	47	theme	weight	466:471	arg1	loss					473:476	weight loss	466:476	weight loss	466:476	The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined.
31382336	3	48	theme	hydrogel	580:587	arg1	potential					553:561	The potential	549:561	The potential of the composite hydrogel for cell vehicle	549:604	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	1	49	theme	double	132:137	arg1	hydrogel					147:154	a double network hydrogel	130:154	a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel	130:207	In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery.
31382336	3	50	attach	isolated	666:673	arg1	rBMSCs					689:694	rBMSCs	689:694	rBMSCs	689:694	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	3	50	attach	isolated	666:673	arg1	rabbits					680:686	rabbits	680:686	rabbits (rBMSCs) within the PoH/GG DNH	680:717	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
31382336	3	50	attach	isolated	666:673	arg2	cells					660:664	bone marrow stem cells	643:664	bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro	643:726	The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro.
29500660	3	0	theme	exopolysaccharides	587:604	arg1	production					606:615	exopolysaccharides production	587:615	exopolysaccharides production from industrial grade starch	587:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	3	1	from	starch	639:644	arg1	production					606:615	exopolysaccharides production	587:615	exopolysaccharides production from industrial grade starch	587:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	2	theme	B.	688:689	arg1	subtilis					691:698	B. subtilis NJ308	688:704	B. subtilis NJ308	688:704	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	0	3	theme	carbon	80:85	arg1	source					87:92	sole carbon source	75:92	sole carbon source	75:92	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	5	4	dep	exopolysaccharides	934:951	arg1	3.41 g/L					964:971	3.41 g/L	964:971	3.41 g/L	964:971	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	5	4	dep	exopolysaccharides	934:951	arg1	titration					953:961	titration	953:961	The maximum exopolysaccharides titration (3.41 g/L)	922:972	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	4	5	theme	key	804:806	arg1	genes					808:812	the related key genes	792:812	the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material	792:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	6	from	material	881:888	arg1	exopolysaccharides					842:859	exopolysaccharides	842:859	exopolysaccharides from the starch raw material	842:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	6	from	material	881:888	arg1	pathway					831:837	the synthesis pathway	817:837	the synthesis pathway of exopolysaccharides from the starch raw material	817:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	7	theme	raw	877:879	arg1	material					881:888	the starch raw material	866:888	the starch raw material	866:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	3	8	theme	up-regulated	390:401	arg1	genes					403:407	up-regulated genes	390:407	up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase	390:524	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	3	8	theme	up-regulated	390:401	arg1	elements					539:546	the key elements	531:546	the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch	531:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	9	theme	starch	870:875	arg1	material					881:888	the starch raw material	866:888	the starch raw material	866:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	10	theme	synthesis	821:829	arg1	pathway					831:837	the synthesis pathway	817:837	the synthesis pathway of exopolysaccharides from the starch raw material	817:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	11	theme	exopolysaccharides	842:859	arg1	pathway					831:837	the synthesis pathway	817:837	the synthesis pathway of exopolysaccharides from the starch raw material	817:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	2	12	theme	raw	306:308	arg1	material					310:317	untreated starch raw material	289:317	untreated starch raw material	289:317	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	2	13	theme	starch	299:304	arg1	material					310:317	untreated starch raw material	289:317	untreated starch raw material	289:317	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	1	14	theme	fermentation	173:184	arg1	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	14	theme	fermentation	173:184	arg1	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	3	15	theme	real-time	350:358	arg1	results					364:370	The real-time PCR results	346:370	The real-time PCR results	346:370	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	1	16	used	used	136:139	arg2	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	16	used	used	136:139	arg2	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	0	17	theme	Enhanced	0:7	arg1	production					9:18	Enhanced production	0:18	Enhanced production of exopolysaccharides	0:40	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	1	18	theme	Industrial	95:104	arg1	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	18	theme	Industrial	95:104	arg1	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	0	19	theme	exopolysaccharides	23:40	arg1	production					9:18	Enhanced production	0:18	Enhanced production of exopolysaccharides	0:40	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	4	20	dep	design	743:748	arg1	methods					779:785	methods	779:785	methods	779:785	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	2	21	theme	sole	326:329	arg1	source					338:343	the sole carbon source	322:343	the sole carbon source	322:343	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	1	22	theme	microbial	200:208	arg1	production					229:238	microbial exopolysaccharides production	200:238	microbial exopolysaccharides production	200:238	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	2	23	theme	carbon	331:336	arg1	source					338:343	the sole carbon source	322:343	the sole carbon source	322:343	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	6	24	theme	71.04 mg/L h	1091:1102	arg1	values					1081:1086	volumetric productivity values	1057:1086	volumetric productivity values of 71.04 mg/L h	1057:1102	This corresponds to volumetric productivity values of 71.04 mg/L h.
29500660	3	25	theme	grade	633:637	arg1	starch					639:644	industrial grade starch	622:644	industrial grade starch	622:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	1	26	theme	soluble	112:118	arg1	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	26	theme	soluble	112:118	arg1	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	5	27	theme	maximum	926:932	arg1	exopolysaccharides					934:951	The maximum exopolysaccharides titration (3.41 g/L)	922:972	The maximum exopolysaccharides titration (3.41 g/L)	922:972	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	1	28	theme	exopolysaccharides	210:227	arg1	production					229:238	microbial exopolysaccharides production	200:238	microbial exopolysaccharides production	200:238	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	0	29	theme	industrial	48:57	arg1	starch					65:70	industrial grade starch	48:70	industrial grade starch	48:70	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	4	30	theme	culture	665:671	arg1	conditions					673:682	the culture conditions	661:682	the culture conditions for B. subtilis NJ308	661:704	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	4	31	theme	composite	762:770	arg1	design					772:777	central composite design	754:777	central composite design	754:777	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	3	32	theme	N-acetylglucosamine-1-phosphate	476:506	arg1	uridyltransferase					508:524	N-acetylglucosamine-1-phosphate uridyltransferase	476:524	N-acetylglucosamine-1-phosphate uridyltransferase	476:524	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	2	33	theme	mutant	259:264	arg1	strain					266:271	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	4	34	theme	central	754:760	arg1	design					772:777	central composite design	754:777	central composite design	754:777	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	5	35	theme	initial	996:1002	arg1	concentration					1011:1023	the initial starch concentration	992:1023	the initial starch concentration	992:1023	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	5	35	theme	initial	996:1002	arg1	45 g/L					1029:1034	45 g/L	1029:1034	45 g/L	1029:1034	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	2	36	theme	subtilis	250:257	arg1	strain					266:271	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	1	37	theme	corn	120:123	arg1	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	37	theme	corn	120:123	arg1	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	0	38	theme	grade	59:63	arg1	starch					65:70	industrial grade starch	48:70	industrial grade starch	48:70	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	6	39	theme	volumetric	1057:1066	arg1	values					1081:1086	volumetric productivity values	1057:1086	volumetric productivity values of 71.04 mg/L h	1057:1102	This corresponds to volumetric productivity values of 71.04 mg/L h.
29500660	2	40	theme	Bacillus	241:248	arg1	strain					266:271	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308	241:277	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	4	41	from	pathway	831:837	arg1	material					881:888	the starch raw material	866:888	the starch raw material	866:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	2	42	theme	untreated	289:297	arg1	material					310:317	untreated starch raw material	289:317	untreated starch raw material	289:317	Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source.
29500660	3	43	theme	mutant	563:568	arg1	strain					570:575	B. subtilis mutant strain NJ308	551:581	B. subtilis mutant strain NJ308	551:581	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	1	44	theme	grade	106:110	arg1	substrate					186:194	the fermentation substrate	169:194	the fermentation substrate	169:194	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	1	44	theme	grade	106:110	arg1	starch					125:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch	95:130	Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production.
29500660	3	45	theme	strain	570:575	arg1	genes					403:407	up-regulated genes	390:407	up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase	390:524	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	3	45	theme	strain	570:575	arg1	elements					539:546	the key elements	531:546	the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch	531:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	0	46	theme	sole	75:78	arg1	source					87:92	sole carbon source	75:92	sole carbon source	75:92	Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
29500660	5	47	theme	starch	1004:1009	arg1	concentration					1011:1023	the initial starch concentration	992:1023	the initial starch concentration	992:1023	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	5	47	theme	starch	1004:1009	arg1	45 g/L					1029:1034	45 g/L	1029:1034	45 g/L	1029:1034	The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L.
29500660	3	48	dep	B.	551:552	arg1	subtilis					554:561	subtilis	554:561	subtilis	554:561	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	3	49	theme	B.	551:552	arg1	strain					570:575	B. subtilis mutant strain NJ308	551:581	B. subtilis mutant strain NJ308	551:581	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	50	theme	related	796:802	arg1	genes					808:812	the related key genes	792:812	the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material	792:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	3	51	theme	key	535:537	arg1	genes					403:407	up-regulated genes	390:407	up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase	390:524	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	3	51	theme	key	535:537	arg1	elements					539:546	the key elements	531:546	the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch	531:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	52	theme	Plackett-Burman	727:741	arg1	design					743:748	Plackett-Burman design	727:748	Plackett-Burman design	727:748	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	3	53	theme	PCR	360:362	arg1	results					364:370	The real-time PCR results	346:370	The real-time PCR results	346:370	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	54	theme	real-time	907:915	arg1	PCR					917:919	real-time PCR	907:919	real-time PCR	907:919	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	3	55	theme	industrial	622:631	arg1	starch					639:644	industrial grade starch	622:644	industrial grade starch	622:644	The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch.
29500660	4	56	from	genes	808:812	arg1	pathway					831:837	the synthesis pathway	817:837	the synthesis pathway of exopolysaccharides from the starch raw material	817:888	Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR.
29500660	6	57	theme	productivity	1068:1079	arg1	values					1081:1086	volumetric productivity values	1057:1086	volumetric productivity values of 71.04 mg/L h	1057:1102	This corresponds to volumetric productivity values of 71.04 mg/L h.
30849091	9	0	theme	apparent	1338:1345	arg1	viscosity					1347:1355	the apparent viscosity	1334:1355	the apparent viscosity	1334:1355	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	9	0	theme	apparent	1338:1345	arg1	size					1374:1377	average droplet size	1358:1377	average droplet size	1358:1377	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	1	1	theme	satisfactory	162:173	arg1	shelf-life					175:184	a satisfactory shelf-life	160:184	a satisfactory shelf-life	160:184	Emulsifiers are added to enhance product stability to obtain a satisfactory shelf-life.
30849091	5	2	dep	stability	686:694	arg1	the					673:675	the	673:675	the	673:675	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	6	3	dep	glycol	840:845	arg1	alginate					847:854	alginate	847:854	alginate	847:854	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	6	3	dep	glycol	840:845	arg1	gum					868:870	xanthan gum	860:870	xanthan gum	860:870	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	5	4	theme	5	726:726	arg1	%					727:727	%	727:727	%	727:727	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	4	5	theme	beneficial	498:507	arg1	effects					509:515	the beneficial effects	494:515	the beneficial effects of ultrasound on the processing efficiency	494:558	In addition, the beneficial effects of ultrasound on the processing efficiency are known.
30849091	5	6	theme	50M	634:636	arg1	MPa					629:631	30 MPa	626:631	30 MPa	626:631	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	6	theme	50M	634:636	arg1	Pa					638:639	50M Pa	634:639	50M Pa	634:639	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	7	from	impact	586:591	arg1	stability					686:694	emulsion stability	677:694	emulsion stability	677:694	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	7	from	impact	586:591	arg1	properties					712:721	emulsifying properties	700:721	emulsifying properties	700:721	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	11	8	theme	beverage	1790:1797	arg1	industries					1799:1808	food and beverage industries	1781:1808	food and beverage industries	1781:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	6	9	theme	propylene	830:838	arg1	glycol					840:845	propylene glycol alginate and xanthan gum	830:870	propylene glycol alginate and xanthan gum	830:870	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	11	10	from	shelf-life	1893:1902	arg1	industries					1925:1934	food and beverage industries	1907:1934	food and beverage industries	1907:1934	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	2	11	theme	food	289:292	arg1	composition					274:284	the fatty acid composition	259:284	the fatty acid composition of food	259:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	5	12	theme	emulsion	677:684	arg1	stability					686:694	emulsion stability	677:694	emulsion stability	677:694	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	7	13	theme	ultrasound	1049:1058	arg1	treatment					1060:1068	ultrasound treatment	1049:1068	ultrasound treatment	1049:1068	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	11	14	theme	food	1888:1891	arg1	shelf-life					1893:1902	food shelf-life	1888:1902	food shelf-life in food and beverage industries	1888:1934	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	9	15	theme	emulsion	1422:1429	arg1	range					1409:1413	the distribution range	1392:1413	the distribution range of the emulsion	1392:1429	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	9	16	theme	ultrasonic	1293:1302	arg1	treatment					1304:1312	ultrasonic treatment	1293:1312	ultrasonic treatment	1293:1312	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	5	17	theme	homogenization	610:623	arg1	impact					586:591	the impact	582:591	the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion	582:757	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	18	theme	high-pressure	596:608	arg1	homogenization					610:623	high-pressure homogenization	596:623	high-pressure homogenization (30 MPa, 50M Pa)	596:640	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	2	19	theme	utmost	353:358	arg1	importance					360:369	utmost importance	353:369	utmost importance	353:369	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	8	20	theme	fluorescence	1193:1204	arg1	microscope					1206:1215	the fluorescence microscope	1189:1215	the fluorescence microscope	1189:1215	The micro structure of the emulsion was observed under the fluorescence microscope.
30849091	0	21	theme	ultrasound	49:58	arg1	power					60:64	ultrasound power	49:64	ultrasound power	49:64	Stability of oil-in-water emulsions performed by ultrasound power or high-pressure homogenization.
30849091	2	22	theme	acid	269:272	arg1	composition					274:284	the fatty acid composition	259:284	the fatty acid composition of food	259:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	23	theme	importance	360:369	arg1	emulsions					211:219	stable emulsions	204:219	stable emulsions that do not form peroxides nor change the fatty acid composition of food	204:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	23	theme	importance	360:369	arg1	treatments					311:320	safe treatments	306:320	safe treatments to obtain them	306:335	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	23	theme	importance	360:369	arg1	aspects					342:348	aspects	342:348	aspects of utmost importance	342:369	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	10	24	theme	ultrasound	1601:1610	arg1	treatment					1612:1620	the ultrasound treatment	1597:1620	the ultrasound treatment	1597:1620	However, aggregation in the emulsion appeared only after being subjected to high-pressure homogenization, while the emulsion made by the ultrasound treatment remained stable during 30 days storage.
30849091	7	25	theme	oil-in-water	1010:1021	arg1	emulsion					1023:1030	5% coconut oil-in-water emulsion	999:1030	5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization	999:1100	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	5	26	theme	emulsifying	700:710	arg1	properties					712:721	emulsifying properties	700:721	emulsifying properties	700:721	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	2	27	dep	treatments	311:320	arg1	obtain					325:330	obtain	325:330	to obtain them	322:335	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	3	28	theme	conventional	406:417	arg1	homogenization					386:399	High-pressure homogenization	372:399	High-pressure homogenization	372:399	High-pressure homogenization is a conventional approach to prepare emulsions because of its high efficiency.
30849091	3	28	theme	conventional	406:417	arg1	approach					419:426	a conventional approach	404:426	a conventional approach to prepare emulsions because of its high efficiency	404:478	High-pressure homogenization is a conventional approach to prepare emulsions because of its high efficiency.
30849091	6	29	theme	glycol	840:845	arg1	emulsifier					889:898	emulsifier	889:898	emulsifier	889:898	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	6	29	theme	glycol	840:845	arg1	complexes					793:801	The complexes	789:801	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum	789:870	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	4	30	theme	processing	538:547	arg1	efficiency					549:558	the processing efficiency	534:558	the processing efficiency	534:558	In addition, the beneficial effects of ultrasound on the processing efficiency are known.
30849091	7	31	theme	emulsion	1023:1030	arg1	ζ-potential					984:994	ζ-potential	984:994	ζ-potential	984:994	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	7	31	theme	emulsion	1023:1030	arg1	distribution					943:954	distribution	943:954	distribution	943:954	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	7	31	theme	emulsion	1023:1030	arg1	viscosity					914:922	The apparent viscosity	901:922	The apparent viscosity	901:922	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	7	31	theme	emulsion	1023:1030	arg1	size					934:937	particle size	925:937	particle size	925:937	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	7	31	theme	emulsion	1023:1030	arg1	properties					969:978	emulsifying properties	957:978	emulsifying properties	957:978	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	11	32	from	industries	1799:1808	arg1	feasible					1769:1776	feasible	1769:1776	feasible	1769:1776	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	11	33	theme	beverage	1916:1923	arg1	industries					1925:1934	food and beverage industries	1907:1934	food and beverage industries	1907:1934	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	0	34	theme	emulsions	26:34	arg1	Stability					0:8	Stability	0:8	Stability of oil-in-water emulsions	0:34	Stability of oil-in-water emulsions performed by ultrasound power or high-pressure homogenization.
30849091	7	35	theme	%	1000:1000	arg1	emulsion					1023:1030	5% coconut oil-in-water emulsion	999:1030	5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization	999:1100	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	10	36	theme	days	1648:1651	arg1	storage					1653:1659	30 days storage	1645:1659	30 days storage	1645:1659	However, aggregation in the emulsion appeared only after being subjected to high-pressure homogenization, while the emulsion made by the ultrasound treatment remained stable during 30 days storage.
30849091	11	37	theme	ultrasound	1849:1858	arg1	performance					1834:1844	a better performance	1825:1844	a better performance of ultrasound	1825:1858	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	0	38	theme	oil-in-water	13:24	arg1	emulsions					26:34	oil-in-water emulsions	13:34	oil-in-water emulsions	13:34	Stability of oil-in-water emulsions performed by ultrasound power or high-pressure homogenization.
30849091	6	39	dep	complexes	793:801	arg1	4:6					811:813	3:7and 4:6	804:813	3:7and 4:6	804:813	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	7	40	theme	coconut	1002:1008	arg1	emulsion					1023:1030	5% coconut oil-in-water emulsion	999:1030	5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization	999:1100	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	11	41	theme	valuable	1697:1704	arg1	information					1706:1716	valuable information	1697:1716	valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries	1697:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	5	42	theme	%	727:727	arg1	emulsion					750:757	5% coconut oil-in-water emulsion	726:757	5% coconut oil-in-water emulsion	726:757	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	7	43	theme	high-pressure	1073:1085	arg1	homogenization					1087:1100	high-pressure homogenization	1073:1100	high-pressure homogenization	1073:1100	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	5	44	theme	coconut	729:735	arg1	emulsion					750:757	5% coconut oil-in-water emulsion	726:757	5% coconut oil-in-water emulsion	726:757	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	7	45	theme	5	999:999	arg1	%					1000:1000	%	1000:1000	%	1000:1000	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	6	46	theme	3:7and	804:809	arg1	4:6					811:813	3:7and 4:6	804:813	3:7and 4:6	804:813	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	6	47	theme	xanthan	860:866	arg1	gum					868:870	xanthan gum	860:870	xanthan gum	860:870	The complexes (3:7and 4:6, by weight) of propylene glycol alginate and xanthan gum were selected as emulsifier.
30849091	3	48	theme	High-pressure	372:384	arg1	homogenization					386:399	High-pressure homogenization	372:399	High-pressure homogenization	372:399	High-pressure homogenization is a conventional approach to prepare emulsions because of its high efficiency.
30849091	3	48	theme	High-pressure	372:384	arg1	approach					419:426	a conventional approach	404:426	a conventional approach to prepare emulsions because of its high efficiency	404:478	High-pressure homogenization is a conventional approach to prepare emulsions because of its high efficiency.
30849091	9	49	theme	average	1358:1364	arg1	viscosity					1347:1355	the apparent viscosity	1334:1355	the apparent viscosity	1334:1355	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	9	49	theme	average	1358:1364	arg1	size					1374:1377	average droplet size	1358:1377	average droplet size	1358:1377	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	5	50	theme	emulsion	750:757	arg1	stability					686:694	emulsion stability	677:694	emulsion stability	677:694	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	50	theme	emulsion	750:757	arg1	properties					712:721	emulsifying properties	700:721	emulsifying properties	700:721	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	10	51	theme	high-pressure	1540:1552	arg1	homogenization					1554:1567	high-pressure homogenization	1540:1567	high-pressure homogenization	1540:1567	However, aggregation in the emulsion appeared only after being subjected to high-pressure homogenization, while the emulsion made by the ultrasound treatment remained stable during 30 days storage.
30849091	7	52	theme	emulsifying	957:967	arg1	properties					969:978	emulsifying properties	957:978	emulsifying properties	957:978	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	4	53	from	effects	509:515	arg1	efficiency					549:558	the processing efficiency	534:558	the processing efficiency	534:558	In addition, the beneficial effects of ultrasound on the processing efficiency are known.
30849091	9	54	theme	droplet	1366:1372	arg1	viscosity					1347:1355	the apparent viscosity	1334:1355	the apparent viscosity	1334:1355	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	9	54	theme	droplet	1366:1372	arg1	size					1374:1377	average droplet size	1358:1377	average droplet size	1358:1377	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	5	55	theme	ultrasound	645:654	arg1	power					656:660	ultrasound power	645:660	ultrasound power (270 W)	645:668	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	55	theme	ultrasound	645:654	arg1	W					667:667	270 W	663:667	270 W	663:667	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	11	56	theme	preparation	1737:1747	arg1	methods					1749:1755	emulsion preparation methods	1728:1755	emulsion preparation methods that can be feasible in food and beverage industries	1728:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	8	57	theme	emulsion	1161:1168	arg1	structure					1144:1152	The micro structure	1134:1152	The micro structure of the emulsion	1134:1168	The micro structure of the emulsion was observed under the fluorescence microscope.
30849091	1	58	theme	product	132:138	arg1	stability					140:148	product stability	132:148	product stability	132:148	Emulsifiers are added to enhance product stability to obtain a satisfactory shelf-life.
30849091	4	59	theme	ultrasound	520:529	arg1	effects					509:515	the beneficial effects	494:515	the beneficial effects of ultrasound on the processing efficiency	494:558	In addition, the beneficial effects of ultrasound on the processing efficiency are known.
30849091	5	60	theme	power	656:660	arg1	impact					586:591	the impact	582:591	the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion	582:757	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	9	61	theme	experimental	1222:1233	arg1	results					1235:1241	The experimental results	1218:1241	The experimental results	1218:1241	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	2	62	theme	fatty	263:267	arg1	composition					274:284	the fatty acid composition	259:284	the fatty acid composition of food	259:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	3	63	theme	high	464:467	arg1	efficiency					469:478	its high efficiency	460:478	its high efficiency	460:478	High-pressure homogenization is a conventional approach to prepare emulsions because of its high efficiency.
30849091	11	64	theme	food	1781:1784	arg1	industries					1799:1808	food and beverage industries	1781:1808	food and beverage industries	1781:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	11	65	from	feasible	1769:1776	arg1	industries					1799:1808	food and beverage industries	1781:1808	food and beverage industries	1781:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	2	66	theme	stable	204:209	arg1	emulsions					211:219	stable emulsions	204:219	stable emulsions that do not form peroxides nor change the fatty acid composition of food	204:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	66	theme	stable	204:209	arg1	treatments					311:320	safe treatments	306:320	safe treatments to obtain them	306:335	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	66	theme	stable	204:209	arg1	aspects					342:348	aspects	342:348	aspects of utmost importance	342:369	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	8	67	theme	micro	1138:1142	arg1	structure					1144:1152	The micro structure	1134:1152	The micro structure of the emulsion	1134:1168	The micro structure of the emulsion was observed under the fluorescence microscope.
30849091	2	68	theme	safe	306:309	arg1	emulsions					211:219	stable emulsions	204:219	stable emulsions that do not form peroxides nor change the fatty acid composition of food	204:292	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	68	theme	safe	306:309	arg1	treatments					311:320	safe treatments	306:320	safe treatments to obtain them	306:335	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	2	68	theme	safe	306:309	arg1	aspects					342:348	aspects	342:348	aspects of utmost importance	342:369	For this reason, stable emulsions that do not form peroxides nor change the fatty acid composition of food, as well as safe treatments to obtain them, are aspects of utmost importance.
30849091	11	69	theme	better	1827:1832	arg1	performance					1834:1844	a better performance	1825:1844	a better performance of ultrasound	1825:1858	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	5	70	dep	homogenization	610:623	arg1	MPa					629:631	30 MPa	626:631	30 MPa	626:631	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	5	70	dep	homogenization	610:623	arg1	Pa					638:639	50M Pa	634:639	50M Pa	634:639	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	0	71	theme	high-pressure	69:81	arg1	homogenization					83:96	high-pressure homogenization	69:96	high-pressure homogenization	69:96	Stability of oil-in-water emulsions performed by ultrasound power or high-pressure homogenization.
30849091	11	72	theme	emulsion	1728:1735	arg1	methods					1749:1755	emulsion preparation methods	1728:1755	emulsion preparation methods that can be feasible in food and beverage industries	1728:1808	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	7	73	theme	particle	925:932	arg1	size					934:937	particle size	925:937	particle size	925:937	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	10	74	from	aggregation	1473:1483	arg1	emulsion					1492:1499	the emulsion	1488:1499	the emulsion	1488:1499	However, aggregation in the emulsion appeared only after being subjected to high-pressure homogenization, while the emulsion made by the ultrasound treatment remained stable during 30 days storage.
30849091	5	75	theme	oil-in-water	737:748	arg1	emulsion					750:757	5% coconut oil-in-water emulsion	726:757	5% coconut oil-in-water emulsion	726:757	Therefore, the impact of high-pressure homogenization (30 MPa, 50M Pa) or ultrasound power (270 W) on the emulsion stability and emulsifying properties of 5% coconut oil-in-water emulsion were discussed in this study.
30849091	9	76	theme	high-pressure	1260:1272	arg1	homogenization					1274:1287	high-pressure homogenization	1260:1287	high-pressure homogenization	1260:1287	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
30849091	7	77	theme	apparent	905:912	arg1	viscosity					914:922	The apparent viscosity	901:922	The apparent viscosity	901:922	The apparent viscosity, particle size and distribution, emulsifying properties and ζ-potential of 5% coconut oil-in-water emulsion before and after ultrasound treatment or high-pressure homogenization were investigated and compared.
30849091	11	78	theme	food	1907:1910	arg1	industries					1925:1934	food and beverage industries	1907:1934	food and beverage industries	1907:1934	In conclusion, this study provides valuable information regarding emulsion preparation methods that can be feasible in food and beverage industries, demonstrating a better performance of ultrasound in optimizing and extending food shelf-life in food and beverage industries.
30849091	9	79	theme	distribution	1396:1407	arg1	range					1409:1413	the distribution range	1392:1413	the distribution range of the emulsion	1392:1429	The experimental results showed that both high-pressure homogenization and ultrasonic treatment effectively reduced the apparent viscosity, average droplet size and narrowed the distribution range of the emulsion, compared with the pre-emulsion.
31005370	10	0	dep	carbohydrates	2001:2013	arg1	The					1997:1999	The	1997:1999	The	1997:1999	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	7	1	theme	chemical	1299:1306	arg1	structures					1308:1317	chemical structures	1299:1317	chemical structures	1299:1317	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	2	theme	Chemical	598:605	arg1	profiles					607:614	Chemical profiles	598:614	METHODS Chemical profiles of WEWG and WERG	590:631	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	2	3	theme	ginseng	369:375	arg1	ginseng					303:309	white ginseng	297:309	white ginseng	297:309	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	2	3	theme	ginseng	369:375	arg1	kinds					336:340	the two kinds	328:340	the two kinds of commonly used processed ginseng	328:375	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	7	4	from	structures	1308:1317	arg1	WEWG					1358:1361	WEWG	1358:1361	WEWG	1358:1361	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	8	5	theme	HFD-fed	1816:1822	arg1	mice					1830:1833	HFD-fed obese mice	1816:1833	HFD-fed obese mice	1816:1833	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	1	6	contain	has	188:190	arg1	Ginseng					180:186	BACKGROUND Ginseng	169:186	BACKGROUND Ginseng	169:186	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	1	6	contain	has	188:190	arg2	potential					204:212	therapeutic potential	192:212	therapeutic potential for treating obesity and the associated gut microbiota dysbiosis	192:277	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	3	7	theme	potential	553:561	arg1	mechanisms					563:572	the potential mechanisms	549:572	the potential mechanisms	549:572	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	7	8	from	compositions	1322:1333	arg1	WEWG					1358:1361	WEWG	1358:1361	WEWG	1358:1361	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	9	theme	content	1499:1505	arg1	ratios					1507:1512	higher content ratios	1492:1512	higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1492:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	10	theme	chromatography-tandem	685:705	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	10	theme	chromatography-tandem	685:705	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	11	11	theme	anti-obesity	2352:2363	arg1	effect					2365:2370	the stronger anti-obesity effect	2339:2370	the stronger anti-obesity effect of WEWG as compared to WERG	2339:2398	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	4	12	theme	quadrupole	714:723	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	12	theme	quadrupole	714:723	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	5	13	theme	systemic	949:956	arg1	inflammation					958:969	systemic inflammation	949:969	systemic inflammation	949:969	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	9	14	theme	anti-obesity	1870:1881	arg1	effect					1883:1888	The stronger anti-obesity effect	1857:1888	The stronger anti-obesity effect of white ginseng	1857:1905	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	7	15	dep	contained	1178:1186	arg1	ratios					1507:1512	higher content ratios	1492:1512	higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1492:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	10	16	theme	beneficial	2160:2169	arg1	effects					2171:2177	more beneficial effects	2155:2177	more beneficial effects of WEWG on the gut microbiota dysbiosis	2155:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	0	17	theme	specificity	138:148	arg1	microbiota					157:166	chemically structural/compositional specificity to gut microbiota	102:166	chemically structural/compositional specificity to gut microbiota	102:166	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	11	18	theme	enteric	2260:2266	arg1	disorders					2278:2286	the enteric metabolic disorders	2256:2286	the enteric metabolic disorders	2256:2286	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	7	19	theme	glucose	1455:1461	arg1	polysaccharides					1475:1489	polysaccharides	1475:1489	polysaccharides	1475:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	19	theme	glucose	1455:1461	arg1	residues					1463:1470	glucose residues	1455:1470	glucose residues of polysaccharides	1455:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	3	20	theme	water	466:470	arg1	extracts					472:479	water extracts	466:479	water extracts	466:479	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	4	21	theme	performance	666:676	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	21	theme	performance	666:676	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	10	22	theme	gut	2194:2196	arg1	dysbiosis					2209:2217	the gut microbiota dysbiosis	2190:2217	the gut microbiota dysbiosis	2190:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	0	23	theme	gut	153:155	arg1	microbiota					157:166	chemically structural/compositional specificity to gut microbiota	102:166	chemically structural/compositional specificity to gut microbiota	102:166	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	1	24	theme	associated	243:252	arg1	dysbiosis					269:277	the associated gut microbiota dysbiosis	239:277	the associated gut microbiota dysbiosis	239:277	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	9	25	theme	ginseng	1899:1905	arg1	effect					1883:1888	The stronger anti-obesity effect	1857:1888	The stronger anti-obesity effect of white ginseng	1857:1905	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	11	26	dep	WEWG	2375:2378	arg1	compared					2383:2390	compared	2383:2390	compared to WERG	2383:2398	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	11	27	theme	systemic	2292:2299	arg1	inflammation					2301:2312	systemic inflammation	2292:2312	systemic inflammation	2292:2312	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	7	28	theme	higher	1432:1437	arg1	ratios					1445:1450	higher molar ratios	1432:1450	higher molar ratios of glucose residues of polysaccharides	1432:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	29	theme	performance	770:780	arg1	chromatography					789:802	high performance liquid chromatography	765:802	high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD)	765:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	5	30	theme	microbiota	1008:1017	arg1	dysbiosis					1019:1027	gut microbiota dysbiosis	1004:1027	gut microbiota dysbiosis	1004:1027	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	1	31	theme	BACKGROUND	169:178	arg1	Ginseng					180:186	BACKGROUND Ginseng	169:186	BACKGROUND Ginseng	169:186	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	7	32	theme	ginsenosides	1281:1292	arg1	contents					1193:1200	less contents	1188:1200	less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides	1188:1292	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	5	33	theme	metabolic	980:988	arg1	disorders					990:998	enteric metabolic disorders	972:998	enteric metabolic disorders	972:998	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	9	34	theme	chemical	1952:1959	arg1	profile					1961:1967	its chemical profile	1948:1967	its chemical profile	1948:1967	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	4	35	theme	light	829:833	arg1	scattering					835:844	evaporative light scattering	817:844	evaporative light scattering detector (HPLC-ELSD)	817:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	2	36	theme	red	315:317	arg1	ginseng					319:325	red ginseng	315:325	red ginseng	315:325	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	5	37	from	inflammation	958:969	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	ginsenosides					1700:1711	high-polar-to-low-polar ginsenosides	1676:1711	high-polar-to-low-polar ginsenosides	1676:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	sucrose-to-melibiose					1629:1648	sucrose-to-melibiose	1629:1648	sucrose-to-melibiose	1629:1648	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	oligosaccharides					1517:1532	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	DP4-7					1582:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7	1534:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides)	1534:1626	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	maltose-to-trehalose					1651:1670	maltose-to-trehalose	1651:1670	maltose-to-trehalose	1651:1670	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	38	dep	oligosaccharides	1517:1532	arg1	tetra-/penta-/hexa-/hepta-saccharides					1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	3	39	theme	extracts	472:479	arg1	effects					455:461	PURPOSE Anti-obesity effects	434:461	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG)	434:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	7	40	theme	free	1255:1258	arg1	monosaccharides					1260:1274	free monosaccharides	1255:1274	free monosaccharides	1255:1274	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	40	theme	free	1255:1258	arg1	polysaccharides					1220:1234	polysaccharides	1220:1234	polysaccharides	1220:1234	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	2	41	contain	possess	378:384	arg1	ginseng					303:309	white ginseng	297:309	white ginseng	297:309	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	2	41	contain	possess	378:384	arg1	ginseng					319:325	red ginseng	315:325	red ginseng	315:325	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	2	41	contain	possess	378:384	arg2	effects					409:415	different anti-obesity effects	386:415	different anti-obesity effects	386:415	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	2	41	contain	possess	378:384	arg1	kinds					336:340	the two kinds	328:340	the two kinds of commonly used processed ginseng	328:375	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	5	42	theme	-fed	1051:1054	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	7	43	theme	weight	1408:1413	arg1	distribution					1415:1426	narrower molecular weight distribution	1389:1426	narrower molecular weight distribution	1389:1426	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	0	44	theme	Stronger	0:7	arg1	effect					22:27	Stronger anti-obesity effect	0:27	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.	0:167	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	5	45	from	disorders	990:998	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	10	46	from	carbohydrates	2001:2013	arg1	WEWG					2035:2038	WEWG	2035:2038	WEWG	2035:2038	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	7	47	theme	narrower	1389:1396	arg1	distribution					1415:1426	narrower molecular weight distribution	1389:1426	narrower molecular weight distribution	1389:1426	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	5	48	theme	WEWG/WERG	892:900	arg1	effects					881:887	Anti-obesity effects	868:887	Anti-obesity effects of WEWG/WERG	868:900	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	7	49	theme	high-polar-to-low-polar	1676:1698	arg1	ginsenosides					1700:1711	high-polar-to-low-polar ginsenosides	1676:1711	high-polar-to-low-polar ginsenosides	1676:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	10	50	from	ginsenosides	2019:2030	arg1	WEWG					2035:2038	WEWG	2035:2038	WEWG	2035:2038	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	3	51	theme	Anti-obesity	442:453	arg1	effects					455:461	PURPOSE Anti-obesity effects	434:461	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG)	434:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	3	52	theme	white	484:488	arg1	ginseng					490:496	white ginseng	484:496	white ginseng	484:496	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	7	53	theme	carbohydrates	1205:1217	arg1	contents					1193:1200	less contents	1188:1200	less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides	1188:1292	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	0	54	theme	white	32:36	arg1	ginseng					38:44	white ginseng	32:44	white ginseng	32:44	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	8	55	theme	fat	1738:1740	arg1	accumulation					1742:1753	fat accumulation	1738:1753	fat accumulation	1738:1753	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	2	56	theme	processed	359:367	arg1	ginseng					369:375	commonly used processed ginseng	345:375	commonly used processed ginseng	345:375	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	9	57	dep	CONCLUSION	1846:1855	arg1	appears					1907:1913	appears	1907:1913	appears to correlate with differences in its chemical profile as compared to red ginseng	1907:1994	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	3	58	theme	ginseng	506:512	arg1	effects					455:461	PURPOSE Anti-obesity effects	434:461	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG)	434:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	8	59	theme	obese	1824:1828	arg1	mice					1830:1833	HFD-fed obese mice	1816:1833	HFD-fed obese mice	1816:1833	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	1	60	theme	gut	254:256	arg1	dysbiosis					269:277	the associated gut microbiota dysbiosis	239:277	the associated gut microbiota dysbiosis	239:277	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	4	61	theme	WEWG	619:622	arg1	profiles					607:614	Chemical profiles	598:614	METHODS Chemical profiles of WEWG and WERG	590:631	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	2	62	theme	different	386:394	arg1	effects					409:415	different anti-obesity effects	386:415	different anti-obesity effects	386:415	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	0	63	theme	red	51:53	arg1	ginseng					55:61	red ginseng	51:61	red ginseng	51:61	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	4	64	theme	WERG	628:631	arg1	profiles					607:614	Chemical profiles	598:614	METHODS Chemical profiles of WEWG and WERG	590:631	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	7	65	theme	-to-oligosaccharides	1561:1580	arg1	DP4-7					1582:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7	1534:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides)	1534:1626	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	65	theme	-to-oligosaccharides	1561:1580	arg1	oligosaccharides					1517:1532	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	65	theme	-to-oligosaccharides	1561:1580	arg1	tetra-/penta-/hexa-/hepta-saccharides					1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	11	66	theme	stronger	2343:2350	arg1	effect					2365:2370	the stronger anti-obesity effect	2339:2370	the stronger anti-obesity effect of WEWG as compared to WERG	2339:2398	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	8	67	theme	microbiota	1792:1801	arg1	dysbiosis					1803:1811	gut microbiota dysbiosis	1788:1811	gut microbiota dysbiosis	1788:1811	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	7	68	theme	di-/tri-saccharides	1541:1559	arg1	DP4-7					1582:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7	1534:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides)	1534:1626	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	68	theme	di-/tri-saccharides	1541:1559	arg1	oligosaccharides					1517:1532	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	68	theme	di-/tri-saccharides	1541:1559	arg1	tetra-/penta-/hexa-/hepta-saccharides					1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	69	theme	oligosaccharides	1517:1532	arg1	ratios					1507:1512	higher content ratios	1492:1512	higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1492:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	1	70	theme	therapeutic	192:202	arg1	potential					204:212	therapeutic potential	192:212	therapeutic potential for treating obesity and the associated gut microbiota dysbiosis	192:277	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	7	71	theme	DP2-3	1534:1538	arg1	DP4-7					1582:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7	1534:1586	DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides)	1534:1626	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	71	theme	DP2-3	1534:1538	arg1	oligosaccharides					1517:1532	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1517:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	71	theme	DP2-3	1534:1538	arg1	tetra-/penta-/hexa-/hepta-saccharides					1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	tetra-/penta-/hexa-/hepta-saccharides	1589:1625	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	11	72	theme	WEWG	2375:2378	arg1	effect					2365:2370	the stronger anti-obesity effect	2339:2370	the stronger anti-obesity effect of WEWG as compared to WERG	2339:2398	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	4	73	theme	liquid	678:683	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	73	theme	liquid	678:683	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	10	74	theme	gut	2132:2134	arg1	bacteria					2136:2143	the obesity-associated gut bacteria	2109:2143	the obesity-associated gut bacteria	2109:2143	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	8	75	from	accumulation	1742:1753	arg1	mice					1830:1833	HFD-fed obese mice	1816:1833	HFD-fed obese mice	1816:1833	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	9	76	theme	stronger	1861:1868	arg1	effect					1883:1888	The stronger anti-obesity effect	1857:1888	The stronger anti-obesity effect of white ginseng	1857:1905	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	7	77	theme	higher	1492:1497	arg1	ratios					1507:1512	higher content ratios	1492:1512	higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides	1492:1711	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	78	theme	triple	707:712	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	78	theme	triple	707:712	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	7	79	dep	distribution	1415:1426	arg1	i.e.					1384:1387	i.e.	1384:1387	i.e.	1384:1387	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	11	80	theme	metabolic	2268:2276	arg1	disorders					2278:2286	the enteric metabolic disorders	2256:2286	the enteric metabolic disorders	2256:2286	This consequently better alleviates the enteric metabolic disorders and systemic inflammation, thereby contributing to the stronger anti-obesity effect of WEWG as compared to WERG.
31005370	7	81	theme	polysaccharides	1475:1489	arg1	polysaccharides					1475:1489	polysaccharides	1475:1489	polysaccharides	1475:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	81	theme	polysaccharides	1475:1489	arg1	residues					1463:1470	glucose residues	1455:1470	glucose residues of polysaccharides	1455:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	10	82	theme	more	2155:2158	arg1	effects					2171:2177	more beneficial effects	2155:2177	more beneficial effects of WEWG on the gut microbiota dysbiosis	2155:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	8	83	from	disorders	1774:1782	arg1	mice					1830:1833	HFD-fed obese mice	1816:1833	HFD-fed obese mice	1816:1833	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	9	84	theme	white	1893:1897	arg1	ginseng					1899:1905	white ginseng	1893:1905	white ginseng	1893:1905	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	7	85	theme	residues	1463:1470	arg1	distribution					1415:1426	narrower molecular weight distribution	1389:1426	narrower molecular weight distribution	1389:1426	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	85	theme	residues	1463:1470	arg1	ratios					1445:1450	higher molar ratios	1432:1450	higher molar ratios of glucose residues of polysaccharides	1432:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	86	theme	ultra-high	655:664	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	86	theme	ultra-high	655:664	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	87	dep	METHODS	590:596	arg1	profiles					607:614	Chemical profiles	598:614	METHODS Chemical profiles of WEWG and WERG	590:631	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	10	88	theme	WEWG	2182:2185	arg1	effects					2171:2177	more beneficial effects	2155:2177	more beneficial effects of WEWG on the gut microbiota dysbiosis	2155:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	8	89	from	dysbiosis	1803:1811	arg1	mice					1830:1833	HFD-fed obese mice	1816:1833	HFD-fed obese mice	1816:1833	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	2	90	theme	white	297:301	arg1	ginseng					303:309	white ginseng	297:309	white ginseng	297:309	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	2	90	theme	white	297:301	arg1	kinds					336:340	the two kinds	328:340	the two kinds of commonly used processed ginseng	328:375	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	5	91	theme	gut	1004:1006	arg1	dysbiosis					1019:1027	gut microbiota dysbiosis	1004:1027	gut microbiota dysbiosis	1004:1027	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	7	92	theme	molar	1439:1443	arg1	ratios					1445:1450	higher molar ratios	1432:1450	higher molar ratios of glucose residues of polysaccharides	1432:1489	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	93	theme	high	765:768	arg1	chromatography					789:802	high performance liquid chromatography	765:802	high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD)	765:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	0	94	dep	microbiota	157:166	arg1	to					150:151	to	150:151	to	150:151	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	8	95	theme	metabolic	1764:1772	arg1	disorders					1774:1782	enteric metabolic disorders	1756:1782	enteric metabolic disorders	1756:1782	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	4	96	theme	mass	725:728	arg1	UHPLC-QqQ-MS/MS					744:758	UHPLC-QqQ-MS/MS	744:758	UHPLC-QqQ-MS/MS	744:758	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	96	theme	mass	725:728	arg1	spectrometry					730:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry	655:741	ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS)	655:759	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	10	97	theme	microbiota	2198:2207	arg1	dysbiosis					2209:2217	the gut microbiota dysbiosis	2190:2217	the gut microbiota dysbiosis	2190:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	0	98	theme	potential	71:79	arg1	mechanisms					81:90	the potential mechanisms	67:90	the potential mechanisms involving chemically structural/compositional specificity to gut microbiota	67:166	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	5	99	from	accumulation	935:946	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	5	100	theme	enteric	972:978	arg1	disorders					990:998	enteric metabolic disorders	972:998	enteric metabolic disorders	972:998	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	6	101	dep	RESULTS	1068:1074	arg1	WEWG					1081:1084	WEWG	1081:1084	WEWG	1081:1084	RESULTS Both WEWG and WERG exerted anti-obesity effects, with WEWG stronger than WERG.
31005370	6	101	dep	RESULTS	1068:1074	arg1	RESULTS					1068:1074	RESULTS Both WEWG and WERG	1068:1093	RESULTS Both WEWG and WERG	1068:1093	RESULTS Both WEWG and WERG exerted anti-obesity effects, with WEWG stronger than WERG.
31005370	6	101	dep	RESULTS	1068:1074	arg1	WERG					1090:1093	WERG	1090:1093	WERG	1090:1093	RESULTS Both WEWG and WERG exerted anti-obesity effects, with WEWG stronger than WERG.
31005370	10	102	from	effects	2171:2177	arg1	dysbiosis					2209:2217	the gut microbiota dysbiosis	2190:2217	the gut microbiota dysbiosis	2190:2217	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	7	103	contain	contained	1178:1186	arg1	WEWG					1173:1176	WEWG	1173:1176	WEWG	1173:1176	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	103	contain	contained	1178:1186	arg2	contents					1193:1200	less contents	1188:1200	less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides	1188:1292	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	3	104	dep	ginseng	506:512	arg1	WEWG					515:518	WEWG	515:518	WEWG	515:518	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	3	104	dep	ginseng	506:512	arg1	WERG					524:527	WERG	524:527	WERG	524:527	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	4	105	theme	evaporative	817:827	arg1	scattering					835:844	evaporative light scattering	817:844	evaporative light scattering detector (HPLC-ELSD)	817:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	5	106	theme	fat	931:933	arg1	accumulation					935:946	fat accumulation	931:946	fat accumulation	931:946	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	0	107	theme	structural/compositional	113:136	arg1	microbiota					157:166	chemically structural/compositional specificity to gut microbiota	102:166	chemically structural/compositional specificity to gut microbiota	102:166	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	7	108	theme	molecular	1398:1406	arg1	distribution					1415:1426	narrower molecular weight distribution	1389:1426	narrower molecular weight distribution	1389:1426	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	109	theme	scattering	835:844	arg1	HPLC-ELSD					856:864	HPLC-ELSD	856:864	HPLC-ELSD	856:864	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	4	109	theme	scattering	835:844	arg1	detector					846:853	evaporative light scattering detector	817:853	evaporative light scattering detector (HPLC-ELSD)	817:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	0	110	theme	anti-obesity	9:20	arg1	effect					22:27	Stronger anti-obesity effect	0:27	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.	0:167	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	9	111	theme	red	1984:1986	arg1	ginseng					1988:1994	red ginseng	1984:1994	red ginseng	1984:1994	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	7	112	dep	carbohydrates	1205:1217	arg1	monosaccharides					1260:1274	free monosaccharides	1255:1274	free monosaccharides	1255:1274	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	112	dep	carbohydrates	1205:1217	arg1	polysaccharides					1220:1234	polysaccharides	1220:1234	polysaccharides	1220:1234	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	112	dep	carbohydrates	1205:1217	arg1	oligosaccharides					1237:1252	oligosaccharides	1237:1252	oligosaccharides	1237:1252	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	4	113	theme	liquid	782:787	arg1	chromatography					789:802	high performance liquid chromatography	765:802	high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD)	765:865	METHODS Chemical profiles of WEWG and WERG were characterized by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) and high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD).
31005370	2	114	theme	anti-obesity	396:407	arg1	effects					409:415	different anti-obesity effects	386:415	different anti-obesity effects	386:415	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	5	115	theme	high-fat	1032:1039	arg1	HFD					1047:1049	HFD	1047:1049	HFD	1047:1049	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	5	115	theme	high-fat	1032:1039	arg1	diet					1041:1044	high-fat diet	1032:1044	high-fat diet (HFD)	1032:1050	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	3	116	theme	red	502:504	arg1	ginseng					506:512	red ginseng	502:512	red ginseng (WEWG and WERG)	502:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	0	117	theme	ginseng	38:44	arg1	effect					22:27	Stronger anti-obesity effect	0:27	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.	0:167	Stronger anti-obesity effect of white ginseng over red ginseng and the potential mechanisms involving chemically structural/compositional specificity to gut microbiota.
31005370	6	118	theme	anti-obesity	1103:1114	arg1	effects					1116:1122	anti-obesity effects	1103:1122	anti-obesity effects	1103:1122	RESULTS Both WEWG and WERG exerted anti-obesity effects, with WEWG stronger than WERG.
31005370	3	119	theme	ginseng	490:496	arg1	effects					455:461	PURPOSE Anti-obesity effects	434:461	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG)	434:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	7	120	theme	less	1188:1191	arg1	contents					1193:1200	less contents	1188:1200	less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides	1188:1292	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	121	theme	components	1344:1353	arg1	compositions					1322:1333	compositions	1322:1333	compositions	1322:1333	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	7	121	theme	components	1344:1353	arg1	structures					1308:1317	chemical structures	1299:1317	chemical structures	1299:1317	Compared to WERG, WEWG contained less contents of carbohydrates (polysaccharides, oligosaccharides, free monosaccharides) and ginsenosides, but chemical structures or compositions of these components in WEWG were characteristic, i.e. narrower molecular weight distribution and higher molar ratios of glucose residues of polysaccharides; higher content ratios of oligosaccharides DP2-3 (di-/tri-saccharides)-to-oligosaccharides DP4-7 (tetra-/penta-/hexa-/hepta-saccharides), sucrose-to-melibiose, maltose-to-trehalose and high-polar-to-low-polar ginsenosides.
31005370	3	122	theme	PURPOSE	434:440	arg1	effects					455:461	PURPOSE Anti-obesity effects	434:461	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG)	434:528	PURPOSE Anti-obesity effects of water extracts of white ginseng and red ginseng (WEWG and WERG) were compared, and the potential mechanisms were discussed.
31005370	8	123	theme	enteric	1756:1762	arg1	disorders					1774:1782	enteric metabolic disorders	1756:1782	enteric metabolic disorders	1756:1782	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	10	124	theme	compositional	2080:2092	arg1	specificity					2094:2104	more structural and compositional specificity	2060:2104	more structural and compositional specificity to the obesity-associated gut bacteria	2060:2143	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	2	125	theme	used	354:357	arg1	ginseng					369:375	commonly used processed ginseng	345:375	commonly used processed ginseng	345:375	However, whether white ginseng and red ginseng, the two kinds of commonly used processed ginseng, possess different anti-obesity effects remains unknown.
31005370	5	126	theme	obese	1056:1060	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	10	127	theme	structural	2065:2074	arg1	specificity					2094:2104	more structural and compositional specificity	2060:2104	more structural and compositional specificity to the obesity-associated gut bacteria	2060:2143	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	5	128	from	dysbiosis	1019:1027	arg1	mice					1062:1065	high-fat diet (HFD)-fed obese mice	1032:1065	high-fat diet (HFD)-fed obese mice	1032:1065	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31005370	1	129	theme	microbiota	258:267	arg1	dysbiosis					269:277	the associated gut microbiota dysbiosis	239:277	the associated gut microbiota dysbiosis	239:277	BACKGROUND Ginseng has therapeutic potential for treating obesity and the associated gut microbiota dysbiosis.
31005370	9	130	from	differences	1933:1943	arg1	profile					1961:1967	its chemical profile	1948:1967	its chemical profile	1948:1967	CONCLUSION The stronger anti-obesity effect of white ginseng appears to correlate with differences in its chemical profile as compared to red ginseng.
31005370	6	131	theme	stronger	1135:1142	arg1	WEWG					1130:1133	WEWG	1130:1133	WEWG stronger than WERG	1130:1152	RESULTS Both WEWG and WERG exerted anti-obesity effects, with WEWG stronger than WERG.
31005370	8	132	theme	gut	1788:1790	arg1	dysbiosis					1803:1811	gut microbiota dysbiosis	1788:1811	gut microbiota dysbiosis	1788:1811	WEWG better ameliorated fat accumulation, enteric metabolic disorders and gut microbiota dysbiosis in HFD-fed obese mice than WERG.
31005370	10	133	theme	obesity-associated	2113:2130	arg1	bacteria					2136:2143	the obesity-associated gut bacteria	2109:2143	the obesity-associated gut bacteria	2109:2143	The carbohydrates and ginsenosides in WEWG potentially present more structural and compositional specificity to the obesity-associated gut bacteria, allowing more beneficial effects of WEWG on the gut microbiota dysbiosis.
31005370	5	134	theme	Anti-obesity	868:879	arg1	effects					881:887	Anti-obesity effects	868:887	Anti-obesity effects of WEWG/WERG	868:900	Anti-obesity effects of WEWG/WERG were examined by determining fat accumulation, systemic inflammation, enteric metabolic disorders and gut microbiota dysbiosis in high-fat diet (HFD)-fed obese mice.
31319145	6	0	from	SCF	794:796	arg1	pronounced					780:789	pronounced	780:789	pronounced	780:789	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	5	1	contain	containing	652:661	arg2	chitosan					663:670	chitosan	663:670	chitosan	663:670	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	5	1	contain	containing	652:661	arg1	Films					646:650	Films	646:650	Films containing chitosan, pectin or guar	646:686	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	5	1	contain	containing	652:661	arg2	guar					683:686	guar	683:686	guar	683:686	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	5	1	contain	containing	652:661	arg2	pectin					673:678	pectin	673:678	pectin	673:678	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	3	2	theme	mass	425:428	arg1	permeability					434:445	permeability	434:445	permeability of films to 5-ASA	434:463	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	2	theme	mass	425:428	arg1	loss					412:415	loss	412:415	loss of film mass	412:428	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	2	theme	mass	425:428	arg1	properties					384:393	The mechanical properties	369:393	The mechanical properties	369:393	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	2	theme	mass	425:428	arg1	index					405:409	swelling index	396:409	swelling index	396:409	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	8	3	from	polysaccharides	1076:1090	arg1	film					1097:1100	a film	1095:1100	a film based on Eudragit RS for colon delivery of 5-ASA	1095:1149	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	1	4	theme	composite	213:221	arg1	film					223:226	a composite film	211:226	a composite film based on Eudragit RS for 5-ASA delivery to the colon	211:279	This study aims at identifying the more suitable polysaccharide in a composite film based on Eudragit RS for 5-ASA delivery to the colon.
31319145	0	5	theme	Eudragit	68:75	arg1	RS					77:78	Eudragit RS	68:78	Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon	68:141	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	4	6	contain	containing	557:566	arg2	dextran					578:584	dextran	578:584	dextran	578:584	Films containing inulin or dextran were more flexible and showed better mechanical properties.
31319145	4	6	contain	containing	557:566	arg2	inulin					568:573	inulin	568:573	inulin	568:573	Films containing inulin or dextran were more flexible and showed better mechanical properties.
31319145	4	6	contain	containing	557:566	arg1	Films					551:555	Films	551:555	Films containing inulin or dextran	551:584	Films containing inulin or dextran were more flexible and showed better mechanical properties.
31319145	3	7	dep	gastric	493:499	arg1	SGF					502:504	SGF	502:504	SGF	502:504	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	0	8	theme	subsequent	84:93	arg1	use					95:97	subsequent use	84:97	subsequent use as a coating for delivery of 5-ASA to colon	84:141	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	0	9	from	Screening	0:8	arg1	film					54:57	a composite film	42:57	a composite film	42:57	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	4	10	theme	mechanical	623:632	arg1	properties					634:643	better mechanical properties	616:643	better mechanical properties	616:643	Films containing inulin or dextran were more flexible and showed better mechanical properties.
31319145	6	11	theme	colonic	855:861	arg1	bacteria					863:870	colonic bacteria	855:870	colonic bacteria	855:870	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	5	12	from	swelling	708:715	arg1	SIF					728:730	SIF	728:730	SIF	728:730	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	5	12	from	swelling	708:715	arg1	SGF					720:722	SGF	720:722	SGF	720:722	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	7	13	theme	highest	970:976	arg1	ratio					978:982	the highest ratio	966:982	the highest ratio of permeability	966:998	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	8	14	theme	Eudragit	1111:1118	arg1	RS					1120:1121	Eudragit RS	1111:1121	Eudragit RS for colon delivery of 5-ASA	1111:1149	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	7	15	contain	containing	888:897	arg2	inulin					899:904	inulin	899:904	inulin	899:904	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	15	contain	containing	888:897	arg1	films					882:886	films	882:886	films containing inulin or dextran	882:915	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	15	contain	containing	888:897	arg2	dextran					909:915	dextran	909:915	dextran	909:915	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	6	16	theme	permeation	760:769	arg1	Loss					733:736	Loss	733:736	Loss of film mass and drug permeation	733:769	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	8	17	theme	colon	1127:1131	arg1	delivery					1133:1140	colon delivery	1127:1140	colon delivery of 5-ASA	1127:1149	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	3	18	theme	films	450:454	arg1	permeability					434:445	permeability	434:445	permeability of films to 5-ASA	434:463	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	18	theme	films	450:454	arg1	loss					412:415	loss	412:415	loss of film mass	412:428	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	18	theme	films	450:454	arg1	properties					384:393	The mechanical properties	369:393	The mechanical properties	369:393	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	18	theme	films	450:454	arg1	index					405:409	swelling index	396:409	swelling index	396:409	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	8	19	theme	appropriate	1064:1074	arg1	dextran					1039:1045	dextran	1039:1045	dextran	1039:1045	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	8	19	theme	appropriate	1064:1074	arg1	inulin					1028:1033	inulin	1028:1033	inulin	1028:1033	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	8	19	theme	appropriate	1064:1074	arg1	polysaccharides					1076:1090	appropriate polysaccharides	1064:1090	appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA	1064:1149	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	6	20	theme	drug	755:758	arg1	permeation					760:769	drug permeation	755:769	drug permeation	755:769	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	3	21	theme	mechanical	373:382	arg1	properties					384:393	The mechanical properties	369:393	The mechanical properties	369:393	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	4	22	theme	better	616:621	arg1	properties					634:643	better mechanical properties	616:643	better mechanical properties	616:643	Films containing inulin or dextran were more flexible and showed better mechanical properties.
31319145	5	23	theme	extensive	698:706	arg1	swelling					708:715	extensive swelling	698:715	extensive swelling in SGF and SIF	698:730	Films containing chitosan, pectin or guar exhibited extensive swelling in SGF and SIF.
31319145	1	24	theme	Eudragit	237:244	arg1	RS					246:247	Eudragit RS	237:247	Eudragit RS for 5-ASA delivery to the colon	237:279	This study aims at identifying the more suitable polysaccharide in a composite film based on Eudragit RS for 5-ASA delivery to the colon.
31319145	3	25	theme	gastric	493:499	arg1	fluids					537:542	gastric (SGF), intestinal (SIF) and colonic fluids	493:542	gastric (SGF), intestinal (SIF) and colonic fluids (SCF)	493:548	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	25	theme	gastric	493:499	arg1	SCF					545:547	SCF	545:547	SCF	545:547	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	0	26	theme	polysaccharides	23:37	arg1	Screening					0:8	Screening	0:8	Screening of different polysaccharides in a composite film	0:57	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	3	27	theme	swelling	396:403	arg1	index					405:409	swelling index	396:409	swelling index	396:409	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	0	28	theme	different	13:21	arg1	polysaccharides					23:37	different polysaccharides	13:37	different polysaccharides	13:37	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	6	29	theme	mass	746:749	arg1	Loss					733:736	Loss	733:736	Loss of film mass and drug permeation	733:769	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	2	30	dep	polysaccharides	292:306	arg1	inulin					333:338	inulin	333:338	inulin	333:338	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	30	dep	polysaccharides	292:306	arg1	pectin					309:314	pectin	309:314	pectin	309:314	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	30	dep	polysaccharides	292:306	arg1	chitosan					317:324	chitosan	317:324	chitosan	317:324	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	30	dep	polysaccharides	292:306	arg1	dextran					345:351	dextran	345:351	dextran	345:351	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	30	dep	polysaccharides	292:306	arg1	polysaccharides					292:306	Different polysaccharides	282:306	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran)	282:352	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	30	dep	polysaccharides	292:306	arg1	guar					327:330	guar	327:330	guar	327:330	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	1	31	theme	5-ASA	253:257	arg1	delivery					259:266	5-ASA delivery	253:266	5-ASA delivery to the colon	253:279	This study aims at identifying the more suitable polysaccharide in a composite film based on Eudragit RS for 5-ASA delivery to the colon.
31319145	7	32	theme	minimum	924:930	arg1	index					941:945	minimum swelling index	924:945	minimum swelling index in SGF and SIF	924:960	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	6	33	theme	film	741:744	arg1	mass					746:749	film mass	741:749	film mass	741:749	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	7	34	theme	swelling	932:939	arg1	index					941:945	minimum swelling index	924:945	minimum swelling index in SGF and SIF	924:960	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	35	theme	permeability	987:998	arg1	ratio					978:982	the highest ratio	966:982	the highest ratio of permeability	966:998	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	35	theme	permeability	987:998	arg1	index					941:945	minimum swelling index	924:945	minimum swelling index in SGF and SIF	924:960	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	0	36	theme	5-ASA	128:132	arg1	delivery					116:123	delivery	116:123	delivery of 5-ASA to colon	116:141	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	3	37	theme	colonic	529:535	arg1	fluids					537:542	gastric (SGF), intestinal (SIF) and colonic fluids	493:542	gastric (SGF), intestinal (SIF) and colonic fluids (SCF)	493:548	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	37	theme	colonic	529:535	arg1	SCF					545:547	SCF	545:547	SCF	545:547	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	0	38	theme	composite	44:52	arg1	film					54:57	a composite film	42:57	a composite film	42:57	Screening of different polysaccharides in a composite film based on Eudragit RS for subsequent use as a coating for delivery of 5-ASA to colon.
31319145	7	39	from	ratio	978:982	arg1	SIF					958:960	SIF	958:960	SIF	958:960	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	39	from	ratio	978:982	arg1	SGF					950:952	SGF	950:952	SGF	950:952	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	3	40	theme	film	420:423	arg1	mass					425:428	film mass	420:428	film mass	420:428	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	2	41	theme	Different	282:290	arg1	inulin					333:338	inulin	333:338	inulin	333:338	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	41	theme	Different	282:290	arg1	pectin					309:314	pectin	309:314	pectin	309:314	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	41	theme	Different	282:290	arg1	chitosan					317:324	chitosan	317:324	chitosan	317:324	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	41	theme	Different	282:290	arg1	dextran					345:351	dextran	345:351	dextran	345:351	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	41	theme	Different	282:290	arg1	polysaccharides					292:306	Different polysaccharides	282:306	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran)	282:352	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	2	41	theme	Different	282:290	arg1	guar					327:330	guar	327:330	guar	327:330	Different polysaccharides (pectin, chitosan, guar, inulin, and dextran) were examined.
31319145	1	42	theme	suitable	184:191	arg1	polysaccharide					193:206	the more suitable polysaccharide	175:206	the more suitable polysaccharide	175:206	This study aims at identifying the more suitable polysaccharide in a composite film based on Eudragit RS for 5-ASA delivery to the colon.
31319145	8	43	theme	5-ASA	1145:1149	arg1	delivery					1133:1140	colon delivery	1127:1140	colon delivery of 5-ASA	1127:1149	Accordingly, inulin and dextran are suggested as appropriate polysaccharides in a film based on Eudragit RS for colon delivery of 5-ASA.
31319145	6	44	from	pronounced	780:789	arg1	SCF					794:796	SCF	794:796	SCF	794:796	Loss of film mass and drug permeation was more pronounced in SCF than SIF for all samples indicating their sensitivity to colonic bacteria.
31319145	3	45	theme	intestinal	508:517	arg1	fluids					537:542	gastric (SGF), intestinal (SIF) and colonic fluids	493:542	gastric (SGF), intestinal (SIF) and colonic fluids (SCF)	493:548	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	45	theme	intestinal	508:517	arg1	SCF					545:547	SCF	545:547	SCF	545:547	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	3	46	dep	intestinal	508:517	arg1	SIF					520:522	SIF	520:522	SIF	520:522	The mechanical properties, swelling index, loss of film mass and permeability of films to 5-ASA were recorded at simulating gastric (SGF), intestinal (SIF) and colonic fluids (SCF).
31319145	7	47	from	index	941:945	arg1	SIF					958:960	SIF	958:960	SIF	958:960	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
31319145	7	47	from	index	941:945	arg1	SGF					950:952	SGF	950:952	SGF	950:952	However, films containing inulin or dextran showed minimum swelling index in SGF and SIF and the highest ratio of permeability in SCF to SIF.
29851976	8	0	theme	membrane	1281:1288	arg1	depolarization					1290:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	0	theme	membrane	1281:1288	arg1	independent					1325:1335	independent	1325:1335	independent	1325:1335	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	4	1	used	used	588:591	arg2	electrodes					572:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	6	2	theme	Myc-LCOs	980:987	arg1	application					956:966	A successive application	943:966	A successive application of sulfated Myc-LCOs and Nod-LCOs	943:1000	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	0	3	theme	truncatula	80:89	arg1	hairs					62:66	root hairs	57:66	root hairs of Medicago truncatula	57:89	Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula.
29851976	7	4	dep	models	1153:1158	arg1	contrast					1133:1140	contrast	1133:1140	contrast	1133:1140	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	1	5	theme	high	174:177	arg1	benefit					179:185	a high benefit	172:185	a high benefit for plant growth and crop production	172:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	6	6	theme	sulfated	971:978	arg1	Myc-LCOs					980:987	sulfated Myc-LCOs	971:987	sulfated Myc-LCOs	971:987	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	3	7	theme	symbiotic	348:356	arg1	responses					358:366	very early symbiotic responses	337:366	very early symbiotic responses of Medicago truncatula root hair cells	337:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	1	8	with	interactions	154:165	arg1	benefit					179:185	a high benefit	172:185	a high benefit for plant growth and crop production	172:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	8	9	theme	cytosolic	1340:1348	arg1	signals					1355:1361	cytosolic Ca2+ signals	1340:1361	cytosolic Ca2+ signals	1340:1361	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	10	theme	Ca2+	1375:1378	arg1	spiking					1380:1386	nuclear Ca2+ spiking	1367:1386	nuclear Ca2+ spiking	1367:1386	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	11	theme	nuclear	1367:1373	arg1	spiking					1380:1386	nuclear Ca2+ spiking	1367:1386	nuclear Ca2+ spiking	1367:1386	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	3	12	with	interaction	512:522	arg1	Myc-LCOs					541:548	Myc-LCOs	541:548	Myc-LCOs	541:548	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	12	with	interaction	512:522	arg1	mycorrhiza					529:538	mycorrhiza	529:538	mycorrhiza (Myc-LCOs)	529:549	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	2	13	theme	developmental	273:285	arg1	process					287:293	the developmental process	269:293	the developmental process of these symbioses in detail	269:322	Thus, it is of great interest to understand the developmental process of these symbioses in detail.
29851976	4	14	theme	potential	711:719	arg1	changes					721:727	membrane potential changes	702:727	membrane potential changes	702:727	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	4	15	theme	Ca2+	685:688	arg1	signals					690:696	cytosolic Ca2+ signals	675:696	cytosolic Ca2+ signals	675:696	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	4	16	theme	membrane	702:709	arg1	changes					721:727	membrane potential changes	702:727	membrane potential changes	702:727	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	4	17	theme	sensitive	619:627	arg1	dyes					638:641	Ca2+ sensitive reporter dyes	614:641	Ca2+ sensitive reporter dyes	614:641	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	8	18	theme	signals	1355:1361	arg1	depolarization					1290:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	18	theme	signals	1355:1361	arg1	independent					1325:1335	independent	1325:1335	independent	1325:1335	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	6	19	theme	single	1021:1026	arg1	depolarization					1038:1051	a single transient depolarization	1019:1051	a single transient depolarization	1019:1051	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	0	20	theme	root	57:60	arg1	hairs					62:66	root hairs	57:66	root hairs of Medicago truncatula	57:89	Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula.
29851976	3	21	theme	root	391:394	arg1	cells					401:405	Medicago truncatula root hair cells	371:405	Medicago truncatula root hair cells	371:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	22	theme	specific	455:462	arg1	lipochitinoligosaccharides					428:453	lipochitinoligosaccharides	428:453	lipochitinoligosaccharides	428:453	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	23	theme	truncatula	380:389	arg1	cells					401:405	Medicago truncatula root hair cells	371:405	Medicago truncatula root hair cells	371:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	5	24	theme	sulfated	744:751	arg1	Myc-					753:756	sulfated Myc-	744:756	sulfated Myc- as well as Nod-LCOs	744:776	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	1	25	theme	Arbuscular	92:101	arg1	Mycorrhiza					103:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	25	theme	Arbuscular	92:101	arg1	Symbiosis					130:138	Root Nodule Symbiosis	118:138	Root Nodule Symbiosis	118:138	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	25	theme	Arbuscular	92:101	arg1	interactions					154:165	symbiotic interactions	144:165	symbiotic interactions with a high benefit for plant growth and crop production	144:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	26	theme	plant	191:195	arg1	growth					197:202	plant growth	191:202	plant growth	191:202	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	0	27	theme	Mycorrhizal	0:10	arg1	LCOs					40:43	LCOs	40:43	LCOs	40:43	Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula.
29851976	0	27	theme	Mycorrhizal	0:10	arg1	lipochitinoligosaccharides					12:37	Mycorrhizal lipochitinoligosaccharides	0:37	Mycorrhizal lipochitinoligosaccharides (LCOs)	0:44	Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula.
29851976	6	28	theme	successive	945:954	arg1	application					956:966	A successive application	943:966	A successive application of sulfated Myc-LCOs and Nod-LCOs	943:1000	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	2	29	theme	symbioses	304:312	arg1	process					287:293	the developmental process	269:293	the developmental process of these symbioses in detail	269:322	Thus, it is of great interest to understand the developmental process of these symbioses in detail.
29851976	5	30	theme	signaling	865:873	arg1	molecules					875:883	these signaling molecules	859:883	these signaling molecules	859:883	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	7	31	theme	current	1145:1151	arg1	models					1153:1158	current models	1145:1158	current models	1145:1158	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	8	32	theme	spiking	1380:1386	arg1	depolarization					1290:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	32	theme	spiking	1380:1386	arg1	independent					1325:1335	independent	1325:1335	independent	1325:1335	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	4	33	theme	reporter	629:636	arg1	dyes					638:641	Ca2+ sensitive reporter dyes	614:641	Ca2+ sensitive reporter dyes	614:641	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	3	34	theme	Medicago	371:378	arg1	cells					401:405	Medicago truncatula root hair cells	371:405	Medicago truncatula root hair cells	371:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	4	35	theme	micro	566:570	arg1	electrodes					572:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	5	36	theme	plants	917:922	arg1	depolarization					798:811	a membrane depolarization	787:811	a membrane depolarization	787:811	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	5	36	theme	plants	917:922	arg1	genotype					901:908	the genotype	897:908	the genotype of the plants that were studied	897:940	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	1	37	theme	Root	118:121	arg1	Mycorrhiza					103:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	37	theme	Root	118:121	arg1	Symbiosis					130:138	Root Nodule Symbiosis	118:138	Root Nodule Symbiosis	118:138	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	37	theme	Root	118:121	arg1	interactions					154:165	symbiotic interactions	144:165	symbiotic interactions with a high benefit for plant growth and crop production	144:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	38	theme	crop	208:211	arg1	production					213:222	crop production	208:222	crop production	208:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	4	39	theme	Intracellular	552:564	arg1	electrodes					572:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes	552:581	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	7	40	theme	root	1244:1247	arg1	cells					1254:1258	root hair cells	1244:1258	root hair cells	1244:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	8	41	theme	Nod-LCO	1265:1271	arg1	depolarization					1290:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	41	theme	Nod-LCO	1265:1271	arg1	independent					1325:1335	independent	1325:1335	independent	1325:1335	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	6	42	theme	Nod-LCOs	993:1000	arg1	application					956:966	A successive application	943:966	A successive application of sulfated Myc-LCOs and Nod-LCOs	943:1000	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	3	43	theme	early	342:346	arg1	responses					358:366	very early symbiotic responses	337:366	very early symbiotic responses of Medicago truncatula root hair cells	337:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	44	theme	hair	396:399	arg1	cells					401:405	Medicago truncatula root hair cells	371:405	Medicago truncatula root hair cells	371:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	5	45	theme	molecules	875:883	arg1	composition					844:854	the chemical composition	831:854	the chemical composition of these signaling molecules	831:883	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	3	46	with	stimulation	411:421	arg1	lipochitinoligosaccharides					428:453	lipochitinoligosaccharides	428:453	lipochitinoligosaccharides	428:453	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	46	with	stimulation	411:421	arg1	interaction					512:522	the interaction	508:522	the interaction with mycorrhiza (Myc-LCOs)	508:549	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	7	47	from	changes	1205:1211	arg1	level					1235:1239	the cytosolic Ca2+ level	1216:1239	the cytosolic Ca2+ level of root hair cells	1216:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	4	48	with	combination	597:607	arg1	dyes					638:641	Ca2+ sensitive reporter dyes	614:641	Ca2+ sensitive reporter dyes	614:641	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	6	49	theme	plasma	1091:1096	arg1	responses					1107:1115	plasma membrane responses	1091:1115	plasma membrane responses to Nod-LCOs	1091:1127	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	1	50	theme	Nodule	123:128	arg1	Mycorrhiza					103:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	50	theme	Nodule	123:128	arg1	Symbiosis					130:138	Root Nodule Symbiosis	118:138	Root Nodule Symbiosis	118:138	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	50	theme	Nodule	123:128	arg1	interactions					154:165	symbiotic interactions	144:165	symbiotic interactions with a high benefit for plant growth and crop production	144:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	3	51	theme	cells	401:405	arg1	responses					358:366	very early symbiotic responses	337:366	very early symbiotic responses of Medicago truncatula root hair cells	337:405	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	7	52	theme	cytosolic	1220:1228	arg1	level					1235:1239	the cytosolic Ca2+ level	1216:1239	the cytosolic Ca2+ level of root hair cells	1216:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	8	53	theme	induced	1273:1279	arg1	depolarization					1290:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization	1261:1303	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	8	53	theme	induced	1273:1279	arg1	independent					1325:1335	independent	1325:1335	independent	1325:1335	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	7	54	theme	hair	1249:1252	arg1	cells					1254:1258	root hair cells	1244:1258	root hair cells	1244:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	3	55	with	lipochitinoligosaccharides	428:453	arg1	Myc-LCOs					541:548	Myc-LCOs	541:548	Myc-LCOs	541:548	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	55	with	lipochitinoligosaccharides	428:453	arg1	mycorrhiza					529:538	mycorrhiza	529:538	mycorrhiza (Myc-LCOs)	529:549	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	3	56	theme	nodules	485:491	arg1	induction					472:480	the induction	468:480	the induction of nodules (Nod-LCOs)	468:502	We analysed very early symbiotic responses of Medicago truncatula root hair cells, by stimulation with lipochitinoligosaccharides specific for the induction of nodules (Nod-LCOs), or the interaction with mycorrhiza (Myc-LCOs).
29851976	6	57	theme	membrane	1098:1105	arg1	responses					1107:1115	plasma membrane responses	1091:1115	plasma membrane responses to Nod-LCOs	1091:1127	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	8	58	theme	Ca2+	1350:1353	arg1	signals					1355:1361	cytosolic Ca2+ signals	1340:1361	cytosolic Ca2+ signals	1340:1361	The Nod-LCO induced membrane depolarization thus is most likely independent of cytosolic Ca2+ signals and nuclear Ca2+ spiking.
29851976	1	59	theme	symbiotic	144:152	arg1	Mycorrhiza					103:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza	92:112	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	59	theme	symbiotic	144:152	arg1	Symbiosis					130:138	Root Nodule Symbiosis	118:138	Root Nodule Symbiosis	118:138	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	1	59	theme	symbiotic	144:152	arg1	interactions					154:165	symbiotic interactions	144:165	symbiotic interactions with a high benefit for plant growth and crop production	144:222	Arbuscular Mycorrhiza and Root Nodule Symbiosis are symbiotic interactions with a high benefit for plant growth and crop production.
29851976	2	60	from	process	287:293	arg1	detail					317:322	detail	317:322	detail	317:322	Thus, it is of great interest to understand the developmental process of these symbioses in detail.
29851976	7	61	theme	cells	1254:1258	arg1	level					1235:1239	the cytosolic Ca2+ level	1216:1239	the cytosolic Ca2+ level of root hair cells	1216:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	0	62	theme	Medicago	71:78	arg1	truncatula					80:89	Medicago truncatula	71:89	Medicago truncatula	71:89	Mycorrhizal lipochitinoligosaccharides (LCOs) depolarize root hairs of Medicago truncatula.
29851976	5	63	theme	chemical	835:842	arg1	composition					844:854	the chemical composition	831:854	the chemical composition of these signaling molecules	831:883	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
29851976	2	64	theme	great	240:244	arg1	interest					246:253	great interest	240:253	great interest	240:253	Thus, it is of great interest to understand the developmental process of these symbioses in detail.
29851976	6	65	theme	transient	1028:1036	arg1	depolarization					1038:1051	a single transient depolarization	1019:1051	a single transient depolarization	1019:1051	A successive application of sulfated Myc-LCOs and Nod-LCOs resulted only in a single transient depolarization, indicating that Myc-LCOs can repress plasma membrane responses to Nod-LCOs.
29851976	4	66	theme	cytosolic	675:683	arg1	signals					690:696	cytosolic Ca2+ signals	675:696	cytosolic Ca2+ signals	675:696	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	4	67	theme	Ca2+	614:617	arg1	dyes					638:641	Ca2+ sensitive reporter dyes	614:641	Ca2+ sensitive reporter dyes	614:641	Intracellular micro electrodes were used, in combination with Ca2+ sensitive reporter dyes, to study the relations between cytosolic Ca2+ signals and membrane potential changes.
29851976	7	68	theme	Ca2+	1230:1233	arg1	level					1235:1239	the cytosolic Ca2+ level	1216:1239	the cytosolic Ca2+ level of root hair cells	1216:1258	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	7	69	theme	Nod-LCO-induced	1165:1179	arg1	depolarization					1181:1194	the Nod-LCO-induced depolarization	1161:1194	the Nod-LCO-induced depolarization	1161:1194	In contrast to current models, the Nod-LCO-induced depolarization precedes changes in the cytosolic Ca2+ level of root hair cells.
29851976	5	70	theme	membrane	789:796	arg1	depolarization					798:811	a membrane depolarization	787:811	a membrane depolarization	787:811	We found that sulfated Myc- as well as Nod-LCOs initiate a membrane depolarization, which depends on the chemical composition of these signaling molecules, as well as the genotype of the plants that were studied.
30391591	0	0	theme	Bletilla	92:99	arg1	striata					101:107	Bletilla striata	92:107	Bletilla striata	92:107	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	5	1	theme	immunocompromised	676:692	arg1	mice					700:703	immunocompromised model mice	676:703	immunocompromised model mice	676:703	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	4	2	link	β‑1,4‑linked	517:528	arg1	residues					541:548	β‑1,4‑linked d‑mannosyl residues	517:548	β‑1,4‑linked d‑mannosyl residues	517:548	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	4	3	theme	linear	469:474	arg1	backbone					476:483	a linear backbone	467:483	a linear backbone	467:483	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	0	4	from	characterization	11:26	arg1	tuber					83:87	the tuber	79:87	the tuber of Bletilla striata	79:107	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	5	5	theme	model	694:698	arg1	mice					700:703	immunocompromised model mice	676:703	immunocompromised model mice	676:703	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	3	6	theme	BSP-2	355:359	arg1	proportion					331:340	proportion	331:340	proportion	331:340	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	3	6	theme	BSP-2	355:359	arg1	composition					315:325	composition	315:325	composition	315:325	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	4	7	link	β‑1,4‑linked	554:565	arg1	residues					578:585	β‑1,4‑linked d‑glucosyl residues	554:585	β‑1,4‑linked d‑glucosyl residues	554:585	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	5	8	theme	mice	700:703	arg1	indices					665:671	the thymus and spleen indices	643:671	the thymus and spleen indices of immunocompromised model mice	643:703	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	0	9	theme	striata	101:107	arg1	tuber					83:87	the tuber	79:87	the tuber of Bletilla striata	79:107	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	3	10	from	mannose	366:372	arg1	ratios					395:400	molar ratios	389:400	molar ratios of 4.0:1.0 and 3.0:1.0	389:423	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	3	11	theme	BSP-1	345:349	arg1	proportion					331:340	proportion	331:340	proportion	331:340	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	3	11	theme	BSP-1	345:349	arg1	composition					315:325	composition	315:325	composition	315:325	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	5	12	theme	thymus	647:652	arg1	indices					665:671	the thymus and spleen indices	643:671	the thymus and spleen indices of immunocompromised model mice	643:703	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	3	13	from	glucose	378:384	arg1	ratios					395:400	molar ratios	389:400	molar ratios of 4.0:1.0 and 3.0:1.0	389:423	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	5	14	theme	spleen	658:663	arg1	indices					665:671	the thymus and spleen indices	643:671	the thymus and spleen indices of immunocompromised model mice	643:703	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	0	15	from	activity	46:53	arg1	tuber					83:87	the tuber	79:87	the tuber of Bletilla striata	79:107	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	0	16	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	4	17	theme	d‑glucosyl	567:576	arg1	residues					578:585	β‑1,4‑linked d‑glucosyl residues	554:585	β‑1,4‑linked d‑glucosyl residues	554:585	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	5	18	theme	immunomodulatory	604:619	arg1	effects					621:627	immunomodulatory effects	604:627	immunomodulatory effects	604:627	BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
30391591	3	19	theme	3.0:1.0	417:423	arg1	ratios					395:400	molar ratios	389:400	molar ratios of 4.0:1.0 and 3.0:1.0	389:423	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	0	20	theme	immunological	32:44	arg1	activity					46:53	immunological activity	32:53	immunological activity	32:53	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	4	21	theme	β‑1,4‑linked	554:565	arg1	residues					578:585	β‑1,4‑linked d‑glucosyl residues	554:585	β‑1,4‑linked d‑glucosyl residues	554:585	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	3	22	dep	composition	315:325	arg1	The					311:313	The	311:313	The	311:313	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	1	23	theme	water-soluble	114:126	arg1	BSP-1					145:149	BSP-1	145:149	BSP-1	145:149	Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata.
30391591	1	23	theme	water-soluble	114:126	arg1	polysaccharides					128:142	Two water-soluble polysaccharides	110:142	Two water-soluble polysaccharides	110:142	Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata.
30391591	1	23	theme	water-soluble	114:126	arg1	BSP-2					155:159	BSP-2	155:159	BSP-2	155:159	Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata.
30391591	4	24	contain	had	463:465	arg1	polysaccharides					447:461	The two polysaccharides	439:461	The two polysaccharides	439:461	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	4	24	contain	had	463:465	arg2	backbone					476:483	a linear backbone	467:483	a linear backbone	467:483	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	1	25	theme	Bletilla	208:215	arg1	striata					217:223	Bletilla striata	208:223	Bletilla striata	208:223	Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata.
30391591	3	26	theme	4.0:1.0	405:411	arg1	ratios					395:400	molar ratios	389:400	molar ratios of 4.0:1.0 and 3.0:1.0	389:423	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	4	27	theme	d‑mannosyl	530:539	arg1	residues					541:548	β‑1,4‑linked d‑mannosyl residues	517:548	β‑1,4‑linked d‑mannosyl residues	517:548	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	0	28	from	tuber	83:87	arg1	polysaccharides					58:72	polysaccharides	58:72	polysaccharides from the tuber of Bletilla striata	58:107	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	0	28	from	tuber	83:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	0	28	from	tuber	83:87	arg1	activity					46:53	immunological activity	32:53	immunological activity	32:53	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	2	29	theme	molecular	230:238	arg1	weights					240:246	The molecular weights	226:246	The molecular weights of BSP-1 and BSP-2	226:265	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
30391591	2	29	theme	molecular	230:238	arg1	83.54 kDa					272:280	83.54 kDa	272:280	83.54 kDa	272:280	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
30391591	1	30	theme	striata	217:223	arg1	tuber					199:203	the tuber	195:203	the tuber of Bletilla striata	195:223	Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata.
30391591	3	31	theme	molar	389:393	arg1	ratios					395:400	molar ratios	389:400	molar ratios of 4.0:1.0 and 3.0:1.0	389:423	The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively.
30391591	4	32	theme	β‑1,4‑linked	517:528	arg1	residues					541:548	β‑1,4‑linked d‑mannosyl residues	517:548	β‑1,4‑linked d‑mannosyl residues	517:548	The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues.
30391591	0	33	theme	polysaccharides	58:72	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	0	33	theme	polysaccharides	58:72	arg1	activity					46:53	immunological activity	32:53	immunological activity	32:53	Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata.
30391591	2	34	theme	BSP-2	261:265	arg1	weights					240:246	The molecular weights	226:246	The molecular weights of BSP-1 and BSP-2	226:265	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
30391591	2	34	theme	BSP-2	261:265	arg1	83.54 kDa					272:280	83.54 kDa	272:280	83.54 kDa	272:280	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
30391591	2	35	theme	BSP-1	251:255	arg1	weights					240:246	The molecular weights	226:246	The molecular weights of BSP-1 and BSP-2	226:265	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
30391591	2	35	theme	BSP-1	251:255	arg1	83.54 kDa					272:280	83.54 kDa	272:280	83.54 kDa	272:280	The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively.
31306470	0	0	theme	soluble	95:101	arg1	mimetics					110:117	stabilized soluble trimer mimetics	84:117	stabilized soluble trimer mimetics	84:117	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	6	1	theme	SOSIP	1169:1173	arg1	trimers					1175:1181	full-length and SOSIP trimers	1153:1181	full-length and SOSIP trimers	1153:1181	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	1	2	theme	broadly	225:231	arg1	bNAbs					258:262	bNAbs	258:262	bNAbs	258:262	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	2	theme	broadly	225:231	arg1	antibodies					246:255	broadly neutralizing antibodies	225:255	broadly neutralizing antibodies (bNAbs)	225:263	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	0	3	theme	stabilized	84:93	arg1	mimetics					110:117	stabilized soluble trimer mimetics	84:117	stabilized soluble trimer mimetics	84:117	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	3	4	theme	early	641:645	arg1	sequence					651:658	an early Env sequence	638:658	an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs	638:712	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	3	4	theme	early	641:645	arg1	AMC011					661:666	AMC011	661:666	AMC011	661:666	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	2	5	theme	SOSIP	327:331	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	1	6	theme	neutralizing	233:244	arg1	bNAbs					258:262	bNAbs	258:262	bNAbs	258:262	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	6	theme	neutralizing	233:244	arg1	antibodies					246:255	broadly neutralizing antibodies	225:255	broadly neutralizing antibodies (bNAbs)	225:263	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	6	7	theme	processed	1278:1286	arg1	glycans					1288:1294	slightly less complex and less extensively processed glycans	1235:1294	slightly less complex and less extensively processed glycans	1235:1294	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	3	8	theme	Env	647:649	arg1	sequence					651:658	an early Env sequence	638:658	an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs	638:712	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	3	8	theme	Env	647:649	arg1	AMC011					661:666	AMC011	661:666	AMC011	661:666	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	6	9	theme	intrinsic	1321:1329	arg1	stabilization					1331:1343	the intrinsic stabilization	1317:1343	the intrinsic stabilization	1317:1343	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	2	10	theme	Recombinant	266:276	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	10	theme	Recombinant	266:276	arg1	mimetics					309:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	3	11	theme	transmembrane	529:541	arg1	domain					543:548	the transmembrane domain	525:548	the transmembrane domain	525:548	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	1	12	theme	antibodies	246:255	arg1	target					215:220	the target	211:220	the target of broadly neutralizing antibodies (bNAbs)	211:263	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	0	13	theme	trimer	103:108	arg1	mimetics					110:117	stabilized soluble trimer mimetics	84:117	stabilized soluble trimer mimetics	84:117	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	7	14	theme	full-length	1473:1483	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	14	theme	full-length	1473:1483	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	5	15	theme	non-neutralizing	1004:1019	arg1	antibodies					1021:1030	non-neutralizing antibodies	1004:1030	non-neutralizing antibodies	1004:1030	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	6	16	theme	trimers	1175:1181	arg1	similar					1187:1193	similar	1187:1193	similar	1187:1193	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	6	16	theme	trimers	1175:1181	arg1	composition					1138:1148	the glycan composition	1127:1148	the glycan composition of full-length and SOSIP trimers	1127:1181	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	3	17	theme	unmodified	492:501	arg1	trimer					507:512	a full-length unmodified Env trimer	478:512	a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	478:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	4	18	theme	bNAb	779:782	arg1	PGT145					784:789	bNAb PGT145	779:789	bNAb PGT145	779:789	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	3	19	from	individual	682:691	arg1	sequence					651:658	an early Env sequence	638:658	an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs	638:712	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	3	19	from	individual	682:691	arg1	AMC011					661:666	AMC011	661:666	AMC011	661:666	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	7	20	theme	membrane-associated	1453:1471	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	20	theme	membrane-associated	1453:1471	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	3	21	theme	Env	503:505	arg1	trimer					507:512	a full-length unmodified Env trimer	478:512	a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	478:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	1	22	theme	virus	198:202	arg1	surface					183:189	the surface	179:189	the surface of the virus	179:202	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	2	23	theme	central	355:361	arg1	role					363:366	a central role	353:366	a central role	353:366	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	5	24	theme	SOSIP	1100:1104	arg1	trimer					1106:1111	the SOSIP trimer	1096:1111	the SOSIP trimer	1096:1111	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	2	25	theme	trimer	302:307	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	25	theme	trimer	302:307	arg1	mimetics					309:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	5	26	theme	intrinsic	1069:1077	arg1	stabilization					1079:1091	the intrinsic stabilization	1065:1091	the intrinsic stabilization of the SOSIP trimer	1065:1111	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	5	27	theme	stabilization	1079:1091	arg1	consequence					1050:1060	a consequence	1048:1060	a consequence of the intrinsic stabilization of the SOSIP trimer	1048:1111	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	6	28	theme	full-length	1153:1163	arg1	trimers					1175:1181	full-length and SOSIP trimers	1153:1181	full-length and SOSIP trimers	1153:1181	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	2	29	theme	Env	298:300	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	29	theme	Env	298:300	arg1	mimetics					309:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	6	30	theme	complex	1249:1255	arg1	glycans					1288:1294	slightly less complex and less extensively processed glycans	1235:1294	slightly less complex and less extensively processed glycans	1235:1294	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	2	31	theme	soluble	290:296	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	31	theme	soluble	290:296	arg1	mimetics					309:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	3	32	theme	HIV+	677:680	arg1	individual					682:691	an HIV+ individual	674:691	an HIV+ individual that developed bNAbs	674:712	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	4	33	theme	trimers	846:852	arg1	structures					826:835	the structures	822:835	the structures of SOSIP trimers	822:852	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	2	34	theme	native-like	278:288	arg1	trimers					333:339	SOSIP trimers	327:339	SOSIP trimers	327:339	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	34	theme	native-like	278:288	arg1	mimetics					309:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics	266:316	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	2	35	theme	vaccine	377:383	arg1	research					385:392	HIV-1 vaccine research	371:392	HIV-1 vaccine research aimed at inducing bNAbs	371:416	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	0	36	theme	HIV-1	44:48	arg1	glycoprotein					59:70	native HIV-1 envelope glycoprotein	37:70	native HIV-1 envelope glycoprotein trimers	37:78	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	3	37	theme	thorough	455:462	arg1	comparison					464:473	a direct and thorough comparison	442:473	a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	442:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	1	38	located	located	168:174	arg2	trimer					158:163	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	38	located	located	168:174	arg1	surface					183:189	the surface	179:189	the surface of the virus	179:202	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	38	located	located	168:174	arg2	located					168:174	located	168:174	located	168:174	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	2	39	theme	HIV-1	371:375	arg1	research					385:392	HIV-1 vaccine research	371:392	HIV-1 vaccine research aimed at inducing bNAbs	371:416	Recombinant native-like soluble Env trimer mimetics, such as SOSIP trimers, have taken a central role in HIV-1 vaccine research aimed at inducing bNAbs.
31306470	0	40	theme	native	37:42	arg1	glycoprotein					59:70	native HIV-1 envelope glycoprotein	37:70	native HIV-1 envelope glycoprotein trimers	37:78	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	7	41	theme	Env	1509:1511	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	41	theme	Env	1509:1511	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	1	42	gly	glycoprotein	139:150	arg1	glycoprotein					139:150	HIV-1 envelope glycoprotein	124:150	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	42	gly	glycoprotein	139:150	arg1	Env					153:155	Env	153:155	Env	153:155	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	3	43	theme	soluble	602:608	arg1	trimer					616:621	soluble SOSIP trimer	602:621	soluble SOSIP trimer	602:621	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	4	44	theme	trimer	756:761	arg1	similar					811:817	similar	811:817	similar	811:817	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	4	44	theme	trimer	756:761	arg1	structures					719:728	The structures	715:728	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151	715:799	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	3	45	dep	matched	594:600	arg1	both					624:627	both	624:627	both	624:627	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	0	46	theme	glycoprotein	59:70	arg1	trimers					72:78	native HIV-1 envelope glycoprotein trimers	37:78	native HIV-1 envelope glycoprotein trimers	37:78	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	3	47	theme	SOSIP	610:614	arg1	trimer					616:621	soluble SOSIP trimer	602:621	soluble SOSIP trimer	602:621	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	6	48	theme	glycan	1131:1136	arg1	similar					1187:1193	similar	1187:1193	similar	1187:1193	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	6	48	theme	glycan	1131:1136	arg1	composition					1138:1148	the glycan composition	1127:1148	the glycan composition of full-length and SOSIP trimers	1127:1181	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	1	49	theme	HIV-1	124:128	arg1	glycoprotein					139:150	HIV-1 envelope glycoprotein	124:150	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	49	theme	HIV-1	124:128	arg1	Env					153:155	Env	153:155	Env	153:155	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	0	50	theme	envelope	50:57	arg1	glycoprotein					59:70	native HIV-1 envelope glycoprotein	37:70	native HIV-1 envelope glycoprotein trimers	37:78	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	3	51	contain	containing	514:523	arg1	trimer					507:512	a full-length unmodified Env trimer	478:512	a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	478:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	3	51	contain	containing	514:523	arg2	domain					543:548	the transmembrane domain	525:548	the transmembrane domain	525:548	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	3	51	contain	containing	514:523	arg2	tail					570:573	the cytoplasmic tail	554:573	the cytoplasmic tail	554:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	6	52	theme	SOSIP	1212:1216	arg1	trimer					1218:1223	the SOSIP trimer	1208:1223	the SOSIP trimer	1208:1223	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	3	53	theme	cytoplasmic	558:568	arg1	tail					570:573	the cytoplasmic tail	554:573	the cytoplasmic tail	554:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	1	54	theme	envelope	130:137	arg1	glycoprotein					139:150	HIV-1 envelope glycoprotein	124:150	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	54	theme	envelope	130:137	arg1	Env					153:155	Env	153:155	Env	153:155	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	6	55	contain	possessed	1225:1233	arg1	trimer					1218:1223	the SOSIP trimer	1208:1223	the SOSIP trimer	1208:1223	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	6	55	contain	possessed	1225:1233	arg2	glycans					1288:1294	slightly less complex and less extensively processed glycans	1235:1294	slightly less complex and less extensively processed glycans	1235:1294	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	6	55	contain	possessed	1225:1233	arg2	absence					1360:1366	the absence	1356:1366	slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether	1235:1389	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	4	56	theme	full-length	737:747	arg1	trimer					756:761	the full-length AMC011 trimer	733:761	the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151	733:799	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	4	57	theme	SOSIP	840:844	arg1	trimers					846:852	SOSIP trimers	840:852	SOSIP trimers	840:852	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	5	58	theme	SOSIP	968:972	arg1	trimer					974:979	the SOSIP trimer	964:979	the SOSIP trimer	964:979	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	5	59	theme	full-length	944:954	arg1	trimer					956:961	the full-length trimer	940:961	the full-length trimer	940:961	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	1	60	theme	glycoprotein	139:150	arg1	located					168:174	located	168:174	located	168:174	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	1	60	theme	glycoprotein	139:150	arg1	trimer					158:163	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer	120:163	The HIV-1 envelope glycoprotein (Env) trimer is located on the surface of the virus and is the target of broadly neutralizing antibodies (bNAbs).
31306470	7	61	theme	SOSIP	1497:1501	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	61	theme	SOSIP	1497:1501	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	3	62	theme	trimer	507:512	arg1	comparison					464:473	a direct and thorough comparison	442:473	a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	442:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	5	63	theme	trimer	1106:1111	arg1	stabilization					1079:1091	the intrinsic stabilization	1065:1091	the intrinsic stabilization of the SOSIP trimer	1065:1111	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	3	64	theme	direct	444:449	arg1	comparison					464:473	a direct and thorough comparison	442:473	a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	442:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	7	65	theme	HIV-1	1503:1507	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	65	theme	HIV-1	1503:1507	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	4	66	theme	AMC011	749:754	arg1	trimer					756:761	the full-length AMC011 trimer	733:761	the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151	733:799	The structures of the full-length AMC011 trimer bound to either bNAb PGT145 or PGT151 were very similar to the structures of SOSIP trimers.
31306470	6	67	dep	membrane	1375:1382	arg1	tether					1384:1389	tether	1384:1389	tether	1384:1389	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	5	68	theme	SOSIP	890:894	arg1	trimers					896:902	SOSIP trimers	890:902	SOSIP trimers	890:902	Antigenically, the full-length and SOSIP trimers were comparable, but in contrast to the full-length trimer, the SOSIP trimer did not bind at all to non-neutralizing antibodies, most likely as a consequence of the intrinsic stabilization of the SOSIP trimer.
31306470	6	69	theme	membrane	1375:1382	arg1	absence					1360:1366	the absence	1356:1366	slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether	1235:1389	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	6	69	theme	membrane	1375:1382	arg1	glycans					1288:1294	slightly less complex and less extensively processed glycans	1235:1294	slightly less complex and less extensively processed glycans	1235:1294	Furthermore, the glycan composition of full-length and SOSIP trimers was similar overall, but the SOSIP trimer possessed slightly less complex and less extensively processed glycans, which may relate to the intrinsic stabilization as well as the absence of the membrane tether.
31306470	0	70	gly	glycoprotein	59:70	arg1	glycoprotein					59:70	native HIV-1 envelope glycoprotein	37:70	native HIV-1 envelope glycoprotein trimers	37:78	Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics.
31306470	3	71	theme	full-length	480:490	arg1	trimer					507:512	a full-length unmodified Env trimer	478:512	a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail	478:573	We therefore performed a direct and thorough comparison of a full-length unmodified Env trimer containing the transmembrane domain and the cytoplasmic tail, with the sequence matched soluble SOSIP trimer, both based on an early Env sequence (AMC011) from an HIV+ individual that developed bNAbs.
31306470	7	72	theme	soluble	1489:1495	arg1	immunogens					1524:1533	immunogens	1524:1533	immunogens	1524:1533	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
31306470	7	72	theme	soluble	1489:1495	arg1	trimers					1513:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	membrane-associated full-length and soluble SOSIP HIV-1 Env trimers	1453:1519	These data provide insights into how to best use and improve membrane-associated full-length and soluble SOSIP HIV-1 Env trimers as immunogens.
30566343	6	0	theme	peptide	1184:1190	arg1	discovery					1147:1155	the initial discovery	1135:1155	the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody	1135:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	6	1	theme	human	1400:1404	arg1	antibodies					1419:1428	different human and nonhuman antibodies	1390:1428	different human and nonhuman antibodies	1390:1428	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	7	2	theme	mass	1542:1545	arg1	spectrometry					1547:1558	mass spectrometry	1542:1558	mass spectrometry experiments	1542:1570	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	0	3	from	Antibodies	182:191	arg1	Conjugates					156:165	Homogeneous Conjugates	144:165	Homogeneous Conjugates from Wild-Type Antibodies	144:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	0	3	from	Antibodies	182:191	arg1	Generation					130:139	Generation	130:139	Generation of Homogeneous Conjugates from Wild-Type Antibodies	130:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	3	4	theme	preparing	615:623	arg1	conjugates					634:643	preparing antibody conjugates	615:643	preparing antibody conjugates	615:643	While such methods are effective, they complicate the process of preparing antibody conjugates and can negatively impact biological activity.
30566343	1	5	theme	homogeneous	311:321	arg1	therapies					332:340	therapies	332:340	therapies	332:340	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	7	6	theme	atomic	1579:1584	arg1	resolution					1586:1595	atomic resolution	1579:1595	atomic resolution	1579:1595	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	6	7	theme	concomitant	1276:1286	arg1	photodamage					1299:1309	concomitant UV-induced photodamage	1276:1309	concomitant UV-induced photodamage of the antibody	1276:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	6	8	theme	photoconjugation	1357:1372	arg1	reaction					1374:1381	the photoconjugation reaction	1353:1381	the photoconjugation reaction	1353:1381	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	8	9	theme	antibody-drug	1840:1852	arg1	conjugate					1854:1862	a conventional antibody-drug conjugate	1825:1862	a conventional antibody-drug conjugate in cells, portending well for future biological applications	1825:1923	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	5	10	theme	phage	989:993	arg1	display					995:1001	phage display	989:1001	phage display	989:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	4	11	theme	robust	760:765	arg1	method					795:800	a robust photoaffinity cross-linking method	758:800	a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies	758:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	2	12	theme	site-specific	364:376	arg1	strategies					390:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	1	13	theme	molecules	229:237	arg1	conjugation					208:218	Site-specific conjugation	194:218	Site-specific conjugation of small molecules to antibodies	194:251	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	3	14	theme	conjugates	634:643	arg1	process					604:610	the process	600:610	the process of preparing antibody conjugates	600:643	While such methods are effective, they complicate the process of preparing antibody conjugates and can negatively impact biological activity.
30566343	5	15	theme	13-residue	957:966	arg1	peptide					968:974	a previously described 13-residue peptide	934:974	a previously described 13-residue peptide derived from phage display	934:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	4	16	theme	detailed	729:736	arg1	characterization					738:753	detailed characterization	729:753	detailed characterization	729:753	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	0	17	theme	Conjugates	156:165	arg1	Generation					130:139	Generation	130:139	Generation of Homogeneous Conjugates from Wild-Type Antibodies	130:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	7	18	theme	conjugation	1479:1489	arg1	site					1471:1474	the specific site	1458:1474	the specific site of conjugation on a human antibody	1458:1509	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	7	18	theme	conjugation	1479:1489	arg1	conjugation					1479:1489	conjugation	1479:1489	conjugation	1479:1489	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	0	19	theme	Wild-Type	172:180	arg1	Antibodies					182:191	Wild-Type Antibodies	172:191	Wild-Type Antibodies	172:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	8	20	theme	future	1894:1899	arg1	applications					1912:1923	future biological applications	1894:1923	future biological applications	1894:1923	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	6	21	theme	nonhuman	1410:1417	arg1	antibodies					1419:1428	different human and nonhuman antibodies	1390:1428	different human and nonhuman antibodies	1390:1428	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	4	22	theme	cross-linking	781:793	arg1	method					795:800	a robust photoaffinity cross-linking method	758:800	a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies	758:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	0	23	theme	Peptide-Based	75:87	arg1	Reaction					117:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction	62:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies	62:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	1	24	theme	Site-specific	194:206	arg1	conjugation					208:218	Site-specific conjugation	194:218	Site-specific conjugation of small molecules to antibodies	194:251	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	0	25	theme	Cross-Linking	103:115	arg1	Reaction					117:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction	62:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies	62:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	7	26	theme	spectrometry	1547:1558	arg1	experiments					1560:1570	mass spectrometry experiments	1542:1570	mass spectrometry experiments	1542:1570	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	2	27	theme	bonds	477:481	arg1	modification					409:420	modification	409:420	modification	409:420	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	27	theme	bonds	477:481	arg1	engineering					486:496	engineering	486:496	engineering of an antibody mutant that bears a reactive handle	486:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	27	theme	bonds	477:481	arg1	removal					425:431	removal	425:431	removal	425:431	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	0	28	theme	Structural	31:40	arg1	Characterization					42:57	Structural Characterization	31:57	Structural Characterization	31:57	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	7	29	from	site	1471:1474	arg1	antibody					1502:1509	a human antibody	1494:1509	a human antibody	1494:1509	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	6	30	theme	effective	1163:1171	arg1	peptide					1184:1190	an effective Bpa mutant peptide	1160:1190	an effective Bpa mutant peptide	1160:1190	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	8	31	theme	photoconjugation	1647:1662	arg1	method					1664:1669	the photoconjugation method	1643:1669	the photoconjugation method	1643:1669	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	4	32	theme	wild-type	854:862	arg1	antibodies					864:873	fully glycosylated wild-type antibodies	835:873	fully glycosylated wild-type antibodies	835:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	2	33	theme	interchain	456:465	arg1	bonds					477:481	interchain disulfide bonds	456:481	interchain disulfide bonds	456:481	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	1	34	theme	targeted	323:330	arg1	therapies					332:340	therapies	332:340	therapies	332:340	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	2	35	theme	mutant	513:518	arg1	modification					409:420	modification	409:420	modification	409:420	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	35	theme	mutant	513:518	arg1	engineering					486:496	engineering	486:496	engineering of an antibody mutant that bears a reactive handle	486:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	35	theme	mutant	513:518	arg1	removal					425:431	removal	425:431	removal	425:431	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	6	36	theme	initial	1139:1145	arg1	discovery					1147:1155	the initial discovery	1135:1155	the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody	1135:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	5	37	theme	bound	1113:1117	arg1	antibody					1119:1126	the bound antibody	1109:1126	the bound antibody	1109:1126	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	2	38	theme	reactive	533:540	arg1	handle					542:547	a reactive handle	531:547	a reactive handle	531:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	6	39	theme	reaction	1216:1223	arg1	conditions					1225:1234	the reaction conditions	1212:1234	the reaction conditions	1212:1234	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	1	40	theme	attractive	267:276	arg1	goal					278:281	an attractive goal	264:281	an attractive goal for the development of more homogeneous targeted therapies and diagnostics	264:356	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	5	41	attach	derived	976:982	arg1	display					995:1001	phage display	989:1001	phage display	989:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	5	41	attach	derived	976:982	arg2	peptide					968:974	a previously described 13-residue peptide	934:974	a previously described 13-residue peptide derived from phage display	934:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	8	42	theme	wild-type	1724:1732	arg1	antibody					1734:1741	a wild-type antibody	1722:1741	a wild-type antibody	1722:1741	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	2	43	theme	glycans	445:451	arg1	modification					409:420	modification	409:420	modification	409:420	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	43	theme	glycans	445:451	arg1	engineering					486:496	engineering	486:496	engineering of an antibody mutant that bears a reactive handle	486:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	43	theme	glycans	445:451	arg1	removal					425:431	removal	425:431	removal	425:431	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	7	44	theme	X-ray	1600:1604	arg1	crystallography					1606:1620	X-ray crystallography	1600:1620	X-ray crystallography	1600:1620	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	6	45	theme	mutant	1177:1182	arg1	peptide					1184:1190	an effective Bpa mutant peptide	1160:1190	an effective Bpa mutant peptide	1160:1190	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	6	46	theme	different	1390:1398	arg1	antibodies					1419:1428	different human and nonhuman antibodies	1390:1428	different human and nonhuman antibodies	1390:1428	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	6	47	theme	UV-induced	1288:1297	arg1	photodamage					1299:1309	concomitant UV-induced photodamage	1276:1309	concomitant UV-induced photodamage of the antibody	1276:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	8	48	theme	resulting	1763:1771	arg1	stable					1791:1796	stable	1791:1796	stable	1791:1796	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	8	48	theme	resulting	1763:1771	arg1	conjugate					1773:1781	the resulting conjugate	1759:1781	the resulting conjugate	1759:1781	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	6	49	theme	reaction	1374:1381	arg1	scope					1344:1348	the scope	1340:1348	the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants	1340:1449	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	2	50	contain	bears	525:529	arg2	handle					542:547	a reactive handle	531:547	a reactive handle	531:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	50	contain	bears	525:529	arg1	mutant					513:518	an antibody mutant	501:518	an antibody mutant that bears a reactive handle	501:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	2	51	theme	conjugation	378:388	arg1	strategies					390:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	8	52	theme	conventional	1827:1838	arg1	conjugate					1854:1862	a conventional antibody-drug conjugate	1825:1862	a conventional antibody-drug conjugate in cells, portending well for future biological applications	1825:1923	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	1	53	theme	small	223:227	arg1	molecules					229:237	small molecules	223:237	small molecules	223:237	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	6	54	theme	efficient	1246:1254	arg1	conjugation					1256:1266	efficient conjugation	1246:1266	efficient conjugation	1246:1266	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	0	55	theme	Homogeneous	144:154	arg1	Conjugates					156:165	Homogeneous Conjugates	144:165	Homogeneous Conjugates from Wild-Type Antibodies	144:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	2	56	theme	Most	359:362	arg1	strategies					390:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies	359:399	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	5	57	theme	described	947:955	arg1	peptide					968:974	a previously described 13-residue peptide	934:974	a previously described 13-residue peptide derived from phage display	934:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	3	58	theme	biological	671:680	arg1	activity					682:689	biological activity	671:689	biological activity	671:689	While such methods are effective, they complicate the process of preparing antibody conjugates and can negatively impact biological activity.
30566343	5	59	theme	peptide	968:974	arg1	mutant					924:929	a benzoylphenylalanine (Bpa) mutant	895:929	a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display	895:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	7	60	theme	human	1496:1500	arg1	antibody					1502:1509	a human antibody	1494:1509	a human antibody	1494:1509	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	4	61	gly	glycosylated	841:852	arg1	antibodies					864:873	fully glycosylated wild-type antibodies	835:873	fully glycosylated wild-type antibodies	835:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	6	62	theme	antibody	1434:1441	arg1	mutants					1443:1449	antibody mutants	1434:1449	antibody mutants	1434:1449	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	5	63	theme	Fc	1026:1027	arg1	domain					1029:1034	the Fc domain	1022:1034	the Fc domain	1022:1034	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	0	64	from	Generation	130:139	arg1	Antibodies					182:191	Wild-Type Antibodies	172:191	Wild-Type Antibodies	172:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	4	65	theme	site-specific	806:818	arg1	conjugation					820:830	site-specific conjugation	806:830	site-specific conjugation to fully glycosylated wild-type antibodies	806:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	3	66	theme	antibody	625:632	arg1	conjugates					634:643	preparing antibody conjugates	615:643	preparing antibody conjugates	615:643	While such methods are effective, they complicate the process of preparing antibody conjugates and can negatively impact biological activity.
30566343	6	67	theme	antibody	1318:1325	arg1	photodamage					1299:1309	concomitant UV-induced photodamage	1276:1309	concomitant UV-induced photodamage of the antibody	1276:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	0	68	theme	Efficient	65:73	arg1	Reaction					117:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction	62:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies	62:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	8	69	theme	biological	1901:1910	arg1	applications					1912:1923	future biological applications	1894:1923	future biological applications	1894:1923	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	7	70	theme	specific	1462:1469	arg1	site					1471:1474	the specific site	1458:1474	the specific site of conjugation on a human antibody	1458:1509	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	7	70	theme	specific	1462:1469	arg1	conjugation					1479:1489	conjugation	1479:1489	conjugation	1479:1489	Next, the specific site of conjugation on a human antibody was characterized in detail by mass spectrometry experiments and at atomic resolution by X-ray crystallography.
30566343	4	71	theme	photoaffinity	767:779	arg1	method					795:800	a robust photoaffinity cross-linking method	758:800	a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies	758:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	0	72	theme	Photoaffinity	89:101	arg1	Reaction					117:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction	62:124	an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies	62:191	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	4	73	theme	method	795:800	arg1	development					713:723	development	713:723	development	713:723	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	4	73	theme	method	795:800	arg1	characterization					738:753	detailed characterization	729:753	detailed characterization	729:753	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	0	74	theme	Reaction	117:124	arg1	Optimization					13:24	Optimization	13:24	Optimization	13:24	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	0	74	theme	Reaction	117:124	arg1	Development					0:10	Development	0:10	Development	0:10	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	0	74	theme	Reaction	117:124	arg1	Characterization					42:57	Structural Characterization	31:57	Structural Characterization	31:57	Development, Optimization, and Structural Characterization of an Efficient Peptide-Based Photoaffinity Cross-Linking Reaction for Generation of Homogeneous Conjugates from Wild-Type Antibodies.
30566343	5	75	theme	Bpa	1062:1064	arg1	residue					1066:1072	the Bpa residue	1058:1072	the Bpa residue	1058:1072	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	8	76	from	stable	1791:1796	arg1	plasma					1801:1806	plasma	1801:1806	plasma	1801:1806	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	6	77	theme	Bpa	1173:1175	arg1	peptide					1184:1190	an effective Bpa mutant peptide	1160:1190	an effective Bpa mutant peptide	1160:1190	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	2	78	theme	disulfide	467:475	arg1	bonds					477:481	interchain disulfide bonds	456:481	interchain disulfide bonds	456:481	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	5	79	theme	UV	1042:1043	arg1	irradiation					1045:1055	UV irradiation	1042:1055	UV irradiation	1042:1055	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	4	80	theme	glycosylated	841:852	arg1	antibodies					864:873	fully glycosylated wild-type antibodies	835:873	fully glycosylated wild-type antibodies	835:873	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	1	81	theme	therapies	332:340	arg1	development					291:301	the development	287:301	the development of more homogeneous targeted therapies and diagnostics	287:356	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	2	82	theme	antibody	504:511	arg1	mutant					513:518	an antibody mutant	501:518	an antibody mutant that bears a reactive handle	501:547	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30566343	8	83	theme	cytotoxic	1683:1691	arg1	payload					1693:1699	a cytotoxic payload	1681:1699	a cytotoxic payload	1681:1699	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	8	84	from	plasma	1801:1806	arg1	stable					1791:1796	stable	1791:1796	stable	1791:1796	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	8	84	from	plasma	1801:1806	arg1	conjugate					1773:1781	the resulting conjugate	1759:1781	the resulting conjugate	1759:1781	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	5	85	theme	benzoylphenylalanine	897:916	arg1	mutant					924:929	a benzoylphenylalanine (Bpa) mutant	895:929	a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display	895:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	1	86	theme	diagnostics	346:356	arg1	development					291:301	the development	287:301	the development of more homogeneous targeted therapies and diagnostics	287:356	Site-specific conjugation of small molecules to antibodies represents an attractive goal for the development of more homogeneous targeted therapies and diagnostics.
30566343	5	87	theme	Bpa	919:921	arg1	mutant					924:929	a benzoylphenylalanine (Bpa) mutant	895:929	a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display	895:1001	The method employs a benzoylphenylalanine (Bpa) mutant of a previously described 13-residue peptide derived from phage display to bind tightly to the Fc domain; upon UV irradiation, the Bpa residue forms a diradical that reacts with the bound antibody.
30566343	4	88	dep	development	713:723	arg1	the					709:711	the	709:711	the	709:711	Herein we report the development and detailed characterization of a robust photoaffinity cross-linking method for site-specific conjugation to fully glycosylated wild-type antibodies.
30566343	6	89	theme	conditions	1225:1234	arg1	optimization					1196:1207	optimization	1196:1207	optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody	1196:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	6	89	theme	conditions	1225:1234	arg1	peptide					1184:1190	an effective Bpa mutant peptide	1160:1190	an effective Bpa mutant peptide	1160:1190	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	3	90	theme	such	556:559	arg1	methods					561:567	such methods	556:567	such methods	556:567	While such methods are effective, they complicate the process of preparing antibody conjugates and can negatively impact biological activity.
30566343	6	91	theme	optimization	1196:1207	arg1	discovery					1147:1155	the initial discovery	1135:1155	the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody	1135:1325	After the initial discovery of an effective Bpa mutant peptide and optimization of the reaction conditions to enable efficient conjugation without concomitant UV-induced photodamage of the antibody, we assessed the scope of the photoconjugation reaction across different human and nonhuman antibodies and antibody mutants.
30566343	8	92	from	conjugate	1854:1862	arg1	cells					1867:1871	cells	1867:1871	cells	1867:1871	Finally, we adapted the photoconjugation method to attach a cytotoxic payload site-specifically to a wild-type antibody and showed that the resulting conjugate is both stable in plasma and as potent as a conventional antibody-drug conjugate in cells, portending well for future biological applications.
30566343	2	93	theme	antibody	436:443	arg1	glycans					445:451	antibody glycans	436:451	antibody glycans	436:451	Most site-specific conjugation strategies require modification or removal of antibody glycans or interchain disulfide bonds or engineering of an antibody mutant that bears a reactive handle.
30807800	9	0	contain	possessed	1084:1092	arg2	-27.3 ± 0.9 mV					1109:1122	-27.3 ± 0.9 mV	1109:1122	-27.3 ± 0.9 mV	1109:1122	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	0	contain	possessed	1084:1092	arg2	charge					1101:1106	a high charge	1094:1106	a high charge (-27.3 ± 0.9 mV)	1094:1123	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	0	contain	possessed	1084:1092	arg1	surface					1064:1070	the surface	1060:1070	the surface of the BGCS	1060:1082	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	10	1	theme	amputation	1342:1351	arg1	models					1353:1358	rat-tail amputation models	1333:1358	rat-tail amputation models	1333:1358	Therefore, the BGCS can stop bleeding within 50 s in rat-tail amputation models.
30807800	1	2	theme	casualties	175:184	arg1	bleeding					119:126	bleeding	119:126	bleeding	119:126	Uncontrolled bleeding is an important cause of military and civilian casualties.
30807800	1	2	theme	casualties	175:184	arg1	cause					144:148	an important cause	131:148	an important cause of military and civilian casualties	131:184	Uncontrolled bleeding is an important cause of military and civilian casualties.
30807800	8	3	theme	evaluation	873:882	arg1	results					838:844	The results	834:844	The results of whole blood coagulation evaluation	834:882	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	11	4	theme	trauma	1444:1449	arg1	hemostasis					1451:1460	trauma hemostasis	1444:1460	trauma hemostasis	1444:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	4	5	theme	Bletilla	505:512	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	5	theme	Bletilla	505:512	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	6	theme	rapid	391:395	arg1	method					410:415	a simple, rapid and low-cost method	381:415	a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	381:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	7	theme	natural	435:441	arg1	polysaccharides					443:457	natural polysaccharides	435:457	natural polysaccharides	435:457	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	11	8	theme	GO	1425:1426	arg1	application					1410:1420	the safe application	1401:1420	the safe application of GO in the field of trauma hemostasis	1401:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	4	9	theme	material	496:503	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	9	theme	material	496:503	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	8	10	theme	excellent	978:986	arg1	effect					999:1004	excellent hemostatic effect	978:1004	excellent hemostatic effect	978:1004	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	11	11	theme	safe	1405:1408	arg1	application					1410:1420	the safe application	1401:1420	the safe application of GO in the field of trauma hemostasis	1401:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	4	12	theme	low-cost	401:408	arg1	method					410:415	a simple, rapid and low-cost method	381:415	a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	381:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	8	13	theme	whole	849:853	arg1	evaluation					873:882	whole blood coagulation evaluation	849:882	whole blood coagulation evaluation	849:882	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	8	14	theme	blood	855:859	arg1	evaluation					873:882	whole blood coagulation evaluation	849:882	whole blood coagulation evaluation	849:882	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	4	15	theme	hydrogen	462:469	arg1	bonding					471:477	hydrogen bonding	462:477	hydrogen bonding	462:477	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	9	16	theme	BGCS	1079:1082	arg1	surface					1064:1070	the surface	1060:1070	the surface of the BGCS	1060:1082	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	10	17	from	50 s	1325:1328	arg1	models					1353:1358	rat-tail amputation models	1333:1358	rat-tail amputation models	1333:1358	Therefore, the BGCS can stop bleeding within 50 s in rat-tail amputation models.
30807800	9	18	theme	Further	1007:1013	arg1	studies					1037:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	19	theme	blood	1194:1198	arg1	cell					1200:1203	red blood cell	1190:1203	red blood cell aggregation	1190:1215	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	20	theme	red	1190:1192	arg1	cell					1200:1203	red blood cell	1190:1203	red blood cell aggregation	1190:1215	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	8	21	theme	coagulation	861:871	arg1	evaluation					873:882	whole blood coagulation evaluation	849:882	whole blood coagulation evaluation	849:882	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	8	22	theme	hemostatic	988:997	arg1	effect					999:1004	excellent hemostatic effect	978:1004	excellent hemostatic effect	978:1004	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	5	23	theme	Zeta	660:663	arg1	potential					665:673	Zeta potential	660:673	Zeta potential	660:673	The BGCS was successfully synthesized and characterized by SEM, IR, RAMAN, XRD and Zeta potential analyzer analysis.
30807800	4	24	theme	simple	383:388	arg1	method					410:415	a simple, rapid and low-cost method	381:415	a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	381:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	7	25	theme	water	807:811	arg1	capacity					824:831	good water absorption capacity	802:831	good water absorption capacity	802:831	Besides, the porosity of BGCS was higher than 90% and showed good water absorption capacity.
30807800	9	26	theme	cell	1200:1203	arg1	aggregation					1205:1215	red blood cell aggregation	1190:1215	red blood cell aggregation	1190:1215	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	7	27	theme	absorption	813:822	arg1	capacity					824:831	good water absorption capacity	802:831	good water absorption capacity	802:831	Besides, the porosity of BGCS was higher than 90% and showed good water absorption capacity.
30807800	2	28	theme	more	203:206	arg1	attention					208:216	more attention	203:216	more attention	203:216	GO has received more attention in the field of hemostasis.
30807800	9	29	theme	high	1096:1099	arg1	charge					1101:1106	a high charge	1094:1106	a high charge (-27.3 ± 0.9 mV)	1094:1123	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	29	theme	high	1096:1099	arg1	-27.3 ± 0.9 mV					1109:1122	-27.3 ± 0.9 mV	1109:1122	-27.3 ± 0.9 mV	1109:1122	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	0	30	theme	striata	39:45	arg1	evaluation					16:25	evaluation	16:25	evaluation of Bletilla striata	16:45	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	0	30	theme	striata	39:45	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	3	31	theme	various	267:273	arg1	limitation					275:284	various limitation	267:284	various limitation	267:284	However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity.
30807800	7	32	theme	good	802:805	arg1	capacity					824:831	good water absorption capacity	802:831	good water absorption capacity	802:831	Besides, the porosity of BGCS was higher than 90% and showed good water absorption capacity.
30807800	0	33	theme	Bletilla	30:37	arg1	striata					39:45	Bletilla striata	30:45	Bletilla striata	30:45	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	4	34	theme	polysaccharide/graphene	522:544	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	34	theme	polysaccharide/graphene	522:544	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	3	35	dep	pure	255:258	arg1	GO					260:261	GO	260:261	GO	260:261	However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity.
30807800	9	36	theme	fibrin	1229:1234	arg1	formation					1236:1244	fibrin formation	1229:1244	fibrin formation	1229:1244	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	10	37	theme	rat-tail	1333:1340	arg1	models					1353:1358	rat-tail amputation models	1333:1358	rat-tail amputation models	1333:1358	Therefore, the BGCS can stop bleeding within 50 s in rat-tail amputation models.
30807800	7	38	theme	BGCS	766:769	arg1	%					789:789	higher than 90%	775:789	higher than 90%	775:789	Besides, the porosity of BGCS was higher than 90% and showed good water absorption capacity.
30807800	7	38	theme	BGCS	766:769	arg1	porosity					754:761	the porosity	750:761	the porosity of BGCS	750:769	Besides, the porosity of BGCS was higher than 90% and showed good water absorption capacity.
30807800	0	39	theme	oxide	71:75	arg1	sponge					98:103	oxide composite hemostatic sponge	71:103	oxide composite hemostatic sponge	71:103	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	4	40	theme	striata	514:520	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	40	theme	striata	514:520	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	11	41	theme	new	1381:1383	arg1	perspective					1385:1395	a new perspective	1379:1395	a new perspective for the safe application of GO in the field of trauma hemostasis	1379:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	2	42	theme	hemostasis	234:243	arg1	field					225:229	the field	221:229	the field of hemostasis	221:243	GO has received more attention in the field of hemostasis.
30807800	1	43	theme	important	134:142	arg1	bleeding					119:126	bleeding	119:126	bleeding	119:126	Uncontrolled bleeding is an important cause of military and civilian casualties.
30807800	1	43	theme	important	134:142	arg1	cause					144:148	an important cause	131:148	an important cause of military and civilian casualties	131:184	Uncontrolled bleeding is an important cause of military and civilian casualties.
30807800	5	44	dep	SEM	636:638	arg1	analysis					684:691	analyzer analysis	675:691	analyzer analysis	675:691	The BGCS was successfully synthesized and characterized by SEM, IR, RAMAN, XRD and Zeta potential analyzer analysis.
30807800	4	45	theme	composite	552:560	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	45	theme	composite	552:560	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	3	46	theme	potential	312:320	arg1	thrombosis					322:331	its potential thrombosis	308:331	its potential thrombosis	308:331	However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity.
30807800	8	47	theme	blood	917:921	arg1	coagulation					923:933	blood coagulation	917:933	blood coagulation	917:933	The results of whole blood coagulation evaluation showed that the BGCS can promote blood coagulation within 30 s without anticoagulant, showing excellent hemostatic effect.
30807800	9	48	theme	coagulation	1015:1025	arg1	studies					1037:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	4	49	theme	oxide	546:550	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	49	theme	oxide	546:550	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	11	50	from	application	1410:1420	arg1	field					1435:1439	the field	1431:1439	the field of trauma hemostasis	1431:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	9	51	theme	strong	1136:1141	arg1	stimulation					1152:1162	strong platelet stimulation	1136:1162	strong platelet stimulation	1136:1162	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	52	theme	mechanism	1027:1035	arg1	studies					1037:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies	1007:1043	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	5	53	theme	analyzer	675:682	arg1	analysis					684:691	analyzer analysis	675:691	analyzer analysis	675:691	The BGCS was successfully synthesized and characterized by SEM, IR, RAMAN, XRD and Zeta potential analyzer analysis.
30807800	9	54	theme	blood	1261:1265	arg1	coagulation					1267:1277	blood coagulation	1261:1277	blood coagulation	1261:1277	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	9	55	theme	platelet	1143:1150	arg1	stimulation					1152:1162	strong platelet stimulation	1136:1162	strong platelet stimulation	1136:1162	Further coagulation mechanism studies indicated that the surface of the BGCS possessed a high charge (-27.3 ± 0.9 mV) and showed strong platelet stimulation, the BGCS can also induce red blood cell aggregation, accelerate fibrin formation and accelerate blood coagulation.
30807800	1	56	theme	military	153:160	arg1	casualties					175:184	military and civilian casualties	153:184	military and civilian casualties	153:184	Uncontrolled bleeding is an important cause of military and civilian casualties.
30807800	3	57	contain	has	263:265	arg1	pure					255:258	pure	255:258	pure	255:258	However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity.
30807800	3	57	contain	has	263:265	arg2	limitation					275:284	various limitation	267:284	various limitation	267:284	However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity.
30807800	11	58	theme	hemostasis	1451:1460	arg1	field					1435:1439	the field	1431:1439	the field of trauma hemostasis	1431:1460	The BGCS provides a new perspective for the safe application of GO in the field of trauma hemostasis.
30807800	0	59	theme	hemostatic	87:96	arg1	sponge					98:103	oxide composite hemostatic sponge	71:103	oxide composite hemostatic sponge	71:103	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	0	60	theme	composite	77:85	arg1	sponge					98:103	oxide composite hemostatic sponge	71:103	oxide composite hemostatic sponge	71:103	Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.
30807800	4	61	theme	new	492:494	arg1	BGCS					570:573	BGCS	570:573	BGCS	570:573	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	4	61	theme	new	492:494	arg1	sponge					562:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge	490:567	a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS)	490:574	Herein, we present a simple, rapid and low-cost method to combine GO and natural polysaccharides by hydrogen bonding to prepare a new material Bletilla striata polysaccharide/graphene oxide composite sponge (BGCS).
30807800	6	62	theme	favorable	713:721	arg1	biocompatibility					723:738	favorable biocompatibility	713:738	favorable biocompatibility	713:738	The BGCS exhibited favorable biocompatibility.
30807800	1	63	theme	civilian	166:173	arg1	casualties					175:184	military and civilian casualties	153:184	military and civilian casualties	153:184	Uncontrolled bleeding is an important cause of military and civilian casualties.
31152126	5	0	theme	bacterial	773:781	arg1	community					783:791	the surrounding bacterial community	757:791	the surrounding bacterial community	757:791	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	6	1	theme	accumulation	973:984	arg1	growth					917:922	the growth	913:922	the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition	913:1048	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	5	2	theme	polysaccharides	640:654	arg1	role					632:635	the role	628:635	the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana	628:736	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	10	3	theme	pseudonana	1532:1541	arg1	growth					1507:1512	growth	1507:1512	growth	1507:1512	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	10	3	theme	pseudonana	1532:1541	arg1	survival					1518:1525	survival	1518:1525	survival	1518:1525	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	9	4	theme	lipase	1423:1428	arg1	abundance					1349:1357	increased abundance	1339:1357	increased abundance of oil-degrading bacterial genera	1339:1391	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	9	4	theme	lipase	1423:1428	arg1	activity					1401:1408	the activity	1397:1408	the activity of exoenzyme lipase	1397:1428	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	7	5	theme	carbon	1079:1084	arg1	reserve					1086:1092	a carbon reserve	1077:1092	a carbon reserve	1077:1092	In addition, by acting as a carbon reserve, chrysolaminarin helped in the recovery of T pseudonana in the presence of oil.
31152126	10	6	theme	chrysolaminarin	1450:1464	arg1	synthesis					1466:1474	chrysolaminarin synthesis	1450:1474	chrysolaminarin synthesis	1450:1474	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	0	7	from	Role	0:3	arg1	Bacteria					78:85	its Associated Bacteria	63:85	its Associated Bacteria in Hydrocarbon Presence	63:109	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	0	7	from	Role	0:3	arg1	pseudonana					48:57	Diatom Thalassiosira pseudonana	27:57	Diatom Thalassiosira pseudonana	27:57	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	0	8	theme	Hydrocarbon	90:100	arg1	Presence					102:109	Hydrocarbon Presence	90:109	Hydrocarbon Presence	90:109	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	6	9	theme	pseudonana	929:938	arg1	growth					917:922	the growth	913:922	the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition	913:1048	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	9	10	theme	bacterial	1376:1384	arg1	genera					1386:1391	oil-degrading bacterial genera	1362:1391	oil-degrading bacterial genera	1362:1391	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	0	11	from	pseudonana	48:57	arg1	Presence					102:109	Hydrocarbon Presence	90:109	Hydrocarbon Presence	90:109	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	10	12	dep	composition	1604:1614	arg1	the					1600:1602	the	1600:1602	the	1600:1602	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	8	13	theme	chrysolaminarin	1188:1202	arg1	synthesis					1204:1212	chrysolaminarin synthesis	1188:1212	chrysolaminarin synthesis	1188:1212	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	10	14	theme	community	1658:1666	arg1	activity					1620:1627	activity	1620:1627	activity	1620:1627	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	10	14	theme	community	1658:1666	arg1	composition					1604:1614	composition	1604:1614	composition	1604:1614	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	7	15	theme	T	1137:1137	arg1	pseudonana					1139:1148	T pseudonana	1137:1148	T pseudonana	1137:1148	In addition, by acting as a carbon reserve, chrysolaminarin helped in the recovery of T pseudonana in the presence of oil.
31152126	3	16	theme	substances	414:423	arg1	production					387:396	Increased production	377:396	Increased production of exopolymeric substances	377:423	Increased production of exopolymeric substances was observed after this oil spill.
31152126	3	17	theme	oil	449:451	arg1	spill					453:457	this oil spill	444:457	this oil spill	444:457	Increased production of exopolymeric substances was observed after this oil spill.
31152126	3	18	theme	Increased	377:385	arg1	production					387:396	Increased production	377:396	Increased production of exopolymeric substances	377:423	Increased production of exopolymeric substances was observed after this oil spill.
31152126	4	19	theme	major	486:490	arg1	fraction					492:499	a major fraction	484:499	a major fraction of exopolymeric substances	484:526	Polysaccharides make up a major fraction of exopolymeric substances; however, their physiological role during an oil spill remains poorly understood.
31152126	2	20	theme	substantial	302:312	arg1	oil					325:327	oil	325:327	oil	325:327	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	2	20	theme	substantial	302:312	arg1	amounts					314:320	substantial amounts	302:320	substantial amounts of oil that also impacted the phytoplankton community	302:374	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	6	21	theme	negative	894:901	arg1	effect					903:908	a negative effect	892:908	a negative effect	892:908	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	8	22	theme	free-living	1261:1271	arg1	fraction					1273:1280	the free-living fraction	1257:1280	the free-living fraction	1257:1280	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	7	23	theme	oil	1169:1171	arg1	presence					1157:1164	the presence	1153:1164	the presence of oil	1153:1171	In addition, by acting as a carbon reserve, chrysolaminarin helped in the recovery of T pseudonana in the presence of oil.
31152126	5	24	from	role	632:635	arg1	physiology					674:683	physiology	674:683	physiology	674:683	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	5	24	from	role	632:635	arg1	growth					663:668	growth	663:668	growth	663:668	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	9	25	theme	genera	1386:1391	arg1	abundance					1349:1357	increased abundance	1339:1357	increased abundance of oil-degrading bacterial genera	1339:1391	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	9	25	theme	genera	1386:1391	arg1	activity					1401:1408	the activity	1397:1408	the activity of exoenzyme lipase	1397:1428	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	6	26	theme	chrysolaminarin	862:876	arg1	synthesis					878:886	chrysolaminarin synthesis	862:886	chrysolaminarin synthesis	862:886	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	8	27	theme	synthesis	1204:1212	arg1	Inhibition					1174:1183	Inhibition	1174:1183	Inhibition of chrysolaminarin synthesis	1174:1212	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	9	28	theme	increased	1339:1347	arg1	abundance					1349:1357	increased abundance	1339:1357	increased abundance of oil-degrading bacterial genera	1339:1391	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	5	29	theme	oil-sensitive	692:704	arg1	pseudonana					727:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	10	30	theme	surrounding	1636:1646	arg1	community					1658:1666	the surrounding bacterial community	1632:1666	the surrounding bacterial community	1632:1666	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	2	31	theme	Mexico	292:297	arg1	Gulf					284:287	the Gulf	280:287	the Gulf of Mexico	280:297	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	1	32	theme	critical	190:197	arg1	polysaccharides					152:166	polysaccharides	152:166	polysaccharides	152:166	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	32	theme	critical	190:197	arg1	amount					142:147	a significant amount	128:147	a significant amount	128:147	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	32	theme	critical	190:197	arg1	source					214:219	a critical organic carbon source	188:219	a critical organic carbon source for bacteria	188:232	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	0	33	theme	Polysaccharides	8:22	arg1	Role					0:3	Role	0:3	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.	0:110	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	4	34	theme	oil	573:575	arg1	spill					577:581	an oil spill	570:581	an oil spill	570:581	Polysaccharides make up a major fraction of exopolymeric substances; however, their physiological role during an oil spill remains poorly understood.
31152126	5	35	theme	diatom	706:711	arg1	pseudonana					727:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	6	36	theme	synthesis	878:886	arg1	inhibition					848:857	inhibition	848:857	inhibition of chrysolaminarin synthesis	848:886	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	7	37	from	recovery	1125:1132	arg1	presence					1157:1164	the presence	1153:1164	the presence of oil	1153:1171	In addition, by acting as a carbon reserve, chrysolaminarin helped in the recovery of T pseudonana in the presence of oil.
31152126	1	38	theme	organic	199:205	arg1	polysaccharides					152:166	polysaccharides	152:166	polysaccharides	152:166	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	38	theme	organic	199:205	arg1	amount					142:147	a significant amount	128:147	a significant amount	128:147	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	38	theme	organic	199:205	arg1	source					214:219	a critical organic carbon source	188:219	a critical organic carbon source for bacteria	188:232	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	0	39	theme	Thalassiosira	34:46	arg1	pseudonana					48:57	Diatom Thalassiosira pseudonana	27:57	Diatom Thalassiosira pseudonana	27:57	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	6	40	theme	monosaccharide	958:971	arg1	accumulation					973:984	intracellular monosaccharide accumulation	944:984	intracellular monosaccharide accumulation	944:984	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	5	41	theme	Thalassiosira	713:725	arg1	pseudonana					727:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	the oil-sensitive diatom Thalassiosira pseudonana	688:736	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	0	42	from	Bacteria	78:85	arg1	Presence					102:109	Hydrocarbon Presence	90:109	Hydrocarbon Presence	90:109	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	1	43	theme	carbon	207:212	arg1	polysaccharides					152:166	polysaccharides	152:166	polysaccharides	152:166	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	43	theme	carbon	207:212	arg1	amount					142:147	a significant amount	128:147	a significant amount	128:147	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	43	theme	carbon	207:212	arg1	source					214:219	a critical organic carbon source	188:219	a critical organic carbon source for bacteria	188:232	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	0	44	theme	Diatom	27:32	arg1	pseudonana					48:57	Diatom Thalassiosira pseudonana	27:57	Diatom Thalassiosira pseudonana	27:57	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	6	45	theme	intracellular	944:956	arg1	accumulation					973:984	intracellular monosaccharide accumulation	944:984	intracellular monosaccharide accumulation	944:984	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	5	46	theme	pseudonana	727:736	arg1	physiology					674:683	physiology	674:683	physiology	674:683	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	5	46	theme	pseudonana	727:736	arg1	growth					663:668	growth	663:668	growth	663:668	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	10	47	theme	oil	1562:1564	arg1	presence					1550:1557	the presence	1546:1557	the presence of oil	1546:1564	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	5	48	dep	analyzed	619:626	arg1	shape					751:755	shape	751:755	shape the surrounding bacterial community and its activity in the presence of oil	751:831	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	9	49	theme	oil-degrading	1362:1374	arg1	genera					1386:1391	oil-degrading bacterial genera	1362:1391	oil-degrading bacterial genera	1362:1391	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
31152126	5	50	theme	oil	829:831	arg1	presence					817:824	the presence	813:824	the presence of oil	813:831	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	10	51	theme	T	1530:1530	arg1	pseudonana					1532:1541	T pseudonana	1530:1541	T pseudonana	1530:1541	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	3	52	theme	exopolymeric	401:412	arg1	substances					414:423	exopolymeric substances	401:423	exopolymeric substances	401:423	Increased production of exopolymeric substances was observed after this oil spill.
31152126	4	53	theme	physiological	544:556	arg1	role					558:561	their physiological role	538:561	their physiological role during an oil spill	538:581	Polysaccharides make up a major fraction of exopolymeric substances; however, their physiological role during an oil spill remains poorly understood.
31152126	5	54	from	activity	801:808	arg1	presence					817:824	the presence	813:824	the presence of oil	813:831	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	10	55	theme	important	1485:1493	arg1	role					1495:1498	an important role	1482:1498	an important role	1482:1498	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	2	56	theme	oil	325:327	arg1	oil					325:327	oil	325:327	oil	325:327	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	2	56	theme	oil	325:327	arg1	amounts					314:320	substantial amounts	302:320	substantial amounts of oil that also impacted the phytoplankton community	302:374	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	6	57	theme	T	927:927	arg1	pseudonana					929:938	T pseudonana	927:938	T pseudonana	927:938	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	7	58	theme	pseudonana	1139:1148	arg1	recovery					1125:1132	the recovery	1121:1132	the recovery of T pseudonana in the presence of oil	1121:1171	In addition, by acting as a carbon reserve, chrysolaminarin helped in the recovery of T pseudonana in the presence of oil.
31152126	5	59	from	community	783:791	arg1	presence					817:824	the presence	813:824	the presence of oil	813:831	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	2	60	theme	oil	262:264	arg1	spill					266:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	8	61	from	community	1244:1252	arg1	phycosphere					1297:1307	the phycosphere	1293:1307	the phycosphere	1293:1307	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	8	61	from	community	1244:1252	arg1	fraction					1273:1280	the free-living fraction	1257:1280	the free-living fraction	1257:1280	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	1	62	theme	significant	130:140	arg1	polysaccharides					152:166	polysaccharides	152:166	polysaccharides	152:166	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	62	theme	significant	130:140	arg1	amount					142:147	a significant amount	128:147	a significant amount	128:147	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	62	theme	significant	130:140	arg1	source					214:219	a critical organic carbon source	188:219	a critical organic carbon source for bacteria	188:232	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	4	63	theme	substances	517:526	arg1	fraction					492:499	a major fraction	484:499	a major fraction of exopolymeric substances	484:526	Polysaccharides make up a major fraction of exopolymeric substances; however, their physiological role during an oil spill remains poorly understood.
31152126	2	64	theme	Horizon	254:260	arg1	spill					266:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	8	65	theme	bacterial	1234:1242	arg1	community					1244:1252	the bacterial community	1230:1252	the bacterial community in the free-living fraction but not in the phycosphere	1230:1307	Inhibition of chrysolaminarin synthesis also influenced the bacterial community in the free-living fraction but not in the phycosphere.
31152126	0	66	theme	Associated	67:76	arg1	Bacteria					78:85	its Associated Bacteria	63:85	its Associated Bacteria in Hydrocarbon Presence	63:109	Role of Polysaccharides in Diatom Thalassiosira pseudonana and its Associated Bacteria in Hydrocarbon Presence.
31152126	2	67	theme	Deepwater	244:252	arg1	spill					266:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	6	68	contain	had	888:890	arg2	effect					903:908	a negative effect	892:908	a negative effect	892:908	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	6	68	contain	had	888:890	arg1	inhibition					848:857	inhibition	848:857	inhibition of chrysolaminarin synthesis	848:886	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	2	69	theme	phytoplankton	352:364	arg1	community					366:374	the phytoplankton community	348:374	the phytoplankton community	348:374	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	10	70	theme	bacterial	1648:1656	arg1	community					1658:1666	the surrounding bacterial community	1632:1666	the surrounding bacterial community	1632:1666	Our data show that chrysolaminarin synthesis plays an important role in the growth and survival of T pseudonana in the presence of oil, and its inhibition can influence the composition and activity of the surrounding bacterial community.
31152126	6	71	theme	feedback	1030:1037	arg1	inhibition					1039:1048	feedback inhibition	1030:1048	feedback inhibition	1030:1048	We found that inhibition of chrysolaminarin synthesis had a negative effect on the growth of T pseudonana and intracellular monosaccharide accumulation, which in turn suppressed photosynthesis by feedback inhibition.
31152126	5	72	theme	surrounding	761:771	arg1	community					783:791	the surrounding bacterial community	757:791	the surrounding bacterial community	757:791	Here, we analyzed the role of polysaccharides in the growth and physiology of the oil-sensitive diatom Thalassiosira pseudonana and how they shape the surrounding bacterial community and its activity in the presence of oil.
31152126	4	73	theme	exopolymeric	504:515	arg1	substances					517:526	exopolymeric substances	504:526	exopolymeric substances	504:526	Polysaccharides make up a major fraction of exopolymeric substances; however, their physiological role during an oil spill remains poorly understood.
31152126	2	74	theme	2010	239:242	arg1	spill					266:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill	235:270	The 2010 Deepwater Horizon oil spill exposed the Gulf of Mexico to substantial amounts of oil that also impacted the phytoplankton community.
31152126	1	75	theme	polysaccharides	152:166	arg1	polysaccharides					152:166	polysaccharides	152:166	polysaccharides	152:166	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	75	theme	polysaccharides	152:166	arg1	amount					142:147	a significant amount	128:147	a significant amount	128:147	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	1	75	theme	polysaccharides	152:166	arg1	source					214:219	a critical organic carbon source	188:219	a critical organic carbon source for bacteria	188:232	Diatoms secrete a significant amount of polysaccharides, which can serve as a critical organic carbon source for bacteria.
31152126	9	76	theme	exoenzyme	1413:1421	arg1	lipase					1423:1428	exoenzyme lipase	1413:1428	exoenzyme lipase	1413:1428	Exposure to oil alone led to increased abundance of oil-degrading bacterial genera and the activity of exoenzyme lipase.
30879690	1	0	theme	neutral	165:171	arg1	NFG					183:185	NFG	183:185	NFG	183:185	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	1	0	theme	neutral	165:171	arg1	fraction					173:180	the flaxseed gum neutral fraction	148:180	the flaxseed gum neutral fraction (NFG)	148:186	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	8	1	theme	natural	1127:1133	arg1	NFG					1076:1078	NFG	1076:1078	NFG from flaxseed gum	1076:1096	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	8	1	theme	natural	1127:1133	arg1	immunomodulator					1135:1149	a natural immunomodulator	1125:1149	a natural immunomodulator in functional foods	1125:1169	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	6	2	theme	IκBα	915:918	arg1	phosphorylation					896:910	phosphorylation	896:910	phosphorylation of IκBα and nuclear factor-κB P65	896:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	5	3	theme	JNK	679:681	arg1	phosphorylation					660:674	increased phosphorylation	650:674	increased phosphorylation of JNK and P38	650:689	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	5	4	theme	P38	619:621	arg1	kinases					600:606	Mitogen-activated protein kinases	574:606	Mitogen-activated protein kinases of JNK and P38	574:621	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	1	5	theme	fraction	173:180	arg1	activity					136:143	Immunostimulatory activity	118:143	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG)	118:186	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	8	6	from	immunomodulator	1135:1149	arg1	foods					1165:1169	functional foods	1154:1169	functional foods	1154:1169	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	0	7	theme	TLR2	101:104	arg1	activation					106:115	TLR2 activation	101:115	TLR2 activation	101:115	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	3	8	theme	phagocytic	377:386	arg1	activity					388:395	phagocytic activity	377:395	phagocytic activity of macrophages	377:410	NFG stimulated NO production and phagocytic activity of macrophages.
30879690	6	9	theme	phosphorylation	896:910	arg1	suppression					855:865	the suppression	851:865	the suppression of IκBα	851:873	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	6	9	theme	phosphorylation	896:910	arg1	up-regulation					879:891	up-regulation	879:891	up-regulation of phosphorylation of IκBα and nuclear factor-κB P65	879:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	5	10	theme	P38	687:689	arg1	phosphorylation					660:674	increased phosphorylation	650:674	increased phosphorylation of JNK and P38	650:689	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	4	11	dep	necrosis	467:474	arg1	2.5 ng/mL					499:507	2.5 ng/mL	499:507	2.5 ng/mL	499:507	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	4	11	dep	necrosis	467:474	arg1	42.9 pg/mL					514:523	42.9 pg/mL	514:523	42.9 pg/mL	514:523	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	4	11	dep	necrosis	467:474	arg1	254.7 pg/mL					486:496	254.7 pg/mL	486:496	254.7 pg/mL	486:496	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	3	12	theme	NO	359:360	arg1	production					362:371	NO production	359:371	NO production	359:371	NFG stimulated NO production and phagocytic activity of macrophages.
30879690	4	13	theme	interleukin-6	426:438	arg1	Secretion					413:421	Secretion	413:421	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively)	413:538	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	5	14	theme	JNK	747:749	arg1	inhibitor					759:767	JNK and P38 inhibitor	747:767	JNK and P38 inhibitor	747:767	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	0	15	from	gum	39:41	arg1	heteroglycan					12:23	Xylose rich heteroglycan	0:23	Xylose rich heteroglycan from flaxseed gum	0:41	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	6	16	theme	factor-κB	792:800	arg1	pathway					812:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	2	17	theme	composition	281:291	arg1	analysis					293:300	monosaccharide composition analysis	266:300	monosaccharide composition analysis	266:300	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	0	18	theme	rich	7:10	arg1	heteroglycan					12:23	Xylose rich heteroglycan	0:23	Xylose rich heteroglycan from flaxseed gum	0:41	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	5	19	theme	increased	650:658	arg1	phosphorylation					660:674	increased phosphorylation	650:674	increased phosphorylation of JNK and P38	650:689	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	6	20	theme	Nuclear	784:790	arg1	pathway					812:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	0	21	theme	Xylose	0:5	arg1	heteroglycan					12:23	Xylose rich heteroglycan	0:23	Xylose rich heteroglycan from flaxseed gum	0:41	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	5	22	theme	JNK	611:613	arg1	kinases					600:606	Mitogen-activated protein kinases	574:606	Mitogen-activated protein kinases of JNK and P38	574:621	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	6	23	theme	signaling	802:810	arg1	pathway					812:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway	784:818	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	6	24	theme	IκBα	870:873	arg1	suppression					855:865	the suppression	851:865	the suppression of IκBα	851:873	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	6	24	theme	IκBα	870:873	arg1	up-regulation					879:891	up-regulation	879:891	up-regulation of phosphorylation of IκBα and nuclear factor-κB P65	879:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	7	25	theme	pathways	1060:1067	arg1	activation					1024:1033	the activation	1020:1033	the activation of down-stream signaling pathways	1020:1067	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	4	26	theme	necrosis	467:474	arg1	Secretion					413:421	Secretion	413:421	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively)	413:538	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	2	27	theme	xylose	233:238	arg1	heteroglycan					245:256	a xylose rich heteroglycan	231:256	a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR	231:341	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	2	27	theme	xylose	233:238	arg1	NFG					206:208	NFG	206:208	NFG	206:208	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	0	28	theme	flaxseed	30:37	arg1	gum					39:41	flaxseed gum	30:41	flaxseed gum	30:41	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	8	29	used	used	1117:1120	arg2	NFG					1076:1078	NFG	1076:1078	NFG from flaxseed gum	1076:1096	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	8	29	used	used	1117:1120	arg2	immunomodulator					1135:1149	a natural immunomodulator	1125:1149	a natural immunomodulator in functional foods	1125:1169	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	0	30	from	effects	74:80	arg1	macrophages					85:95	macrophages	85:95	macrophages	85:95	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	8	31	theme	functional	1154:1163	arg1	foods					1165:1169	functional foods	1154:1169	functional foods	1154:1169	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	7	32	theme	down-stream	1038:1048	arg1	pathways					1060:1067	down-stream signaling pathways	1038:1067	down-stream signaling pathways	1038:1067	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	5	33	theme	P38	755:757	arg1	inhibitor					759:767	JNK and P38 inhibitor	747:767	JNK and P38 inhibitor	747:767	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	1	34	theme	Immunostimulatory	118:134	arg1	activity					136:143	Immunostimulatory activity	118:143	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG)	118:186	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	8	35	theme	flaxseed	1085:1092	arg1	gum					1094:1096	flaxseed gum	1085:1096	flaxseed gum	1085:1096	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	6	36	theme	P65	942:944	arg1	phosphorylation					896:910	phosphorylation	896:910	phosphorylation of IκBα and nuclear factor-κB P65	896:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	7	37	theme	signaling	1050:1058	arg1	pathways					1060:1067	down-stream signaling pathways	1038:1067	down-stream signaling pathways	1038:1067	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	5	38	theme	Mitogen-activated	574:590	arg1	kinases					600:606	Mitogen-activated protein kinases	574:606	Mitogen-activated protein kinases of JNK and P38	574:621	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	6	39	theme	factor-κB	932:940	arg1	P65					942:944	nuclear factor-κB P65	924:944	nuclear factor-κB P65	924:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	5	40	theme	NO	698:699	arg1	production					701:710	NO production	698:710	NO production	698:710	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	4	41	theme	interleukin-1β	441:454	arg1	Secretion					413:421	Secretion	413:421	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively)	413:538	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	2	42	theme	monosaccharide	266:279	arg1	analysis					293:300	monosaccharide composition analysis	266:300	monosaccharide composition analysis	266:300	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	5	43	theme	protein	592:598	arg1	kinases					600:606	Mitogen-activated protein kinases	574:606	Mitogen-activated protein kinases of JNK and P38	574:621	Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively.
30879690	8	44	from	gum	1094:1096	arg1	NFG					1076:1078	NFG	1076:1078	NFG from flaxseed gum	1076:1096	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	8	44	from	gum	1094:1096	arg1	immunomodulator					1135:1149	a natural immunomodulator	1125:1149	a natural immunomodulator in functional foods	1125:1169	Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
30879690	4	45	theme	tumor	461:465	arg1	necrosis					467:474	tumor necrosis factor-α	461:483	tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively)	461:538	Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG.
30879690	1	46	theme	gum	161:163	arg1	NFG					183:185	NFG	183:185	NFG	183:185	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	1	46	theme	gum	161:163	arg1	fraction					173:180	the flaxseed gum neutral fraction	148:180	the flaxseed gum neutral fraction (NFG)	148:186	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	7	47	theme	NFG	996:998	arg1	Toll					947:950	Toll	947:950	Toll like receptor-2	947:966	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	7	47	theme	NFG	996:998	arg1	target					986:991	the molecular target	972:991	the molecular target of NFG	972:998	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	1	48	theme	flaxseed	152:159	arg1	NFG					183:185	NFG	183:185	NFG	183:185	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	1	48	theme	flaxseed	152:159	arg1	fraction					173:180	the flaxseed gum neutral fraction	148:180	the flaxseed gum neutral fraction (NFG)	148:186	Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated.
30879690	0	49	theme	immunostimulatory	56:72	arg1	effects					74:80	the immunostimulatory effects	52:80	the immunostimulatory effects on macrophages	52:95	Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation.
30879690	6	50	theme	nuclear	924:930	arg1	P65					942:944	nuclear factor-κB P65	924:944	nuclear factor-κB P65	924:944	Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65.
30879690	2	51	theme	rich	240:243	arg1	heteroglycan					245:256	a xylose rich heteroglycan	231:256	a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR	231:341	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	2	51	theme	rich	240:243	arg1	NFG					206:208	NFG	206:208	NFG	206:208	NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR.
30879690	7	52	theme	molecular	976:984	arg1	Toll					947:950	Toll	947:950	Toll like receptor-2	947:966	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	7	52	theme	molecular	976:984	arg1	target					986:991	the molecular target	972:991	the molecular target of NFG	972:998	Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways.
30879690	3	53	theme	macrophages	400:410	arg1	production					362:371	NO production	359:371	NO production	359:371	NFG stimulated NO production and phagocytic activity of macrophages.
30879690	3	53	theme	macrophages	400:410	arg1	activity					388:395	phagocytic activity	377:395	phagocytic activity of macrophages	377:410	NFG stimulated NO production and phagocytic activity of macrophages.
30320131	9	0	theme	groups	1383:1388	arg1	distribution					1352:1363	the distribution	1348:1363	the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C)	1348:1421	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	1	from	similarity	1334:1343	arg1	distribution					1352:1363	the distribution	1348:1363	the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C)	1348:1421	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	7	2	theme	different	1132:1140	arg1	fractions					1146:1154	the different EPS fractions	1128:1154	the different EPS fractions	1128:1154	The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa.
30320131	4	3	theme	EPS	705:707	arg1	fractions					709:717	the EPS fractions	701:717	the EPS fractions	701:717	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	9	4	dep	infrared	1298:1305	arg1	FTIR					1308:1311	FTIR	1308:1311	FTIR	1308:1311	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	6	5	dep	C7	990:991	arg1	B3					1023:1024	B3	1023:1024	B3	1023:1024	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	3	6	theme	Leuconostoc	644:654	arg1	mesenteroides					656:668	Leuconostoc mesenteroides B3	644:671	Leuconostoc mesenteroides B3	644:671	However, it was similar with both methods for Leuconostoc mesenteroides B3.
30320131	0	7	from	Evaluation	0:9	arg1	Characteristics					98:112	Characteristics	98:112	Characteristics	98:112	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	0	7	from	Evaluation	0:9	arg1	Purification					81:92	Purification	81:92	Purification	81:92	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	6	8	theme	Leuconostoc	997:1007	arg1	mesenteroides					1009:1021	Leuconostoc mesenteroides	997:1021	Leuconostoc mesenteroides	997:1021	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	2	9	theme	ethanol	515:521	arg1	precipitation					523:535	ethanol precipitation	515:535	ethanol precipitation	515:535	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	1	10	theme	10	442:443	arg1	KDa					445:447	KDa	445:447	KDa	445:447	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	11	theme	Lactobacillus	275:287	arg1	C7					299:300	Lactobacillus plantarum C7	275:300	Lactobacillus plantarum C7	275:300	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	11	theme	Lactobacillus	275:287	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	0	12	theme	Exopolysaccharides	117:134	arg1	Characteristics					98:112	Characteristics	98:112	Characteristics	98:112	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	0	12	theme	Exopolysaccharides	117:134	arg1	Purification					81:92	Purification	81:92	Purification	81:92	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	7	13	theme	molecular	1107:1115	arg1	weights					1117:1123	The molecular weights	1103:1123	The molecular weights of the different EPS fractions	1103:1154	The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa.
30320131	1	14	theme	KDa	445:447	arg1	membrane					457:464	a 10 KDa cut-off membrane	440:464	a 10 KDa cut-off membrane	440:464	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	4	15	theme	studied	787:793	arg1	methods					795:801	the two studied methods	779:801	the two studied methods	779:801	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	6	16	theme	different	1079:1087	arg1	ratios					1095:1100	different molar ratios	1079:1100	different molar ratios	1079:1100	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	8	17	theme	EPS-UF	1262:1267	arg1	fractions					1269:1277	EPS-UF fractions	1262:1277	EPS-UF fractions	1262:1277	Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions.
30320131	2	18	dep	lactis	553:558	arg1	lactis					553:558	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	2	18	dep	lactis	553:558	arg1	SLT10					560:564	SLT10	560:564	SLT10	560:564	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	2	18	dep	lactis	553:558	arg1	C7					594:595	Lactobacillus plantarum C7	570:595	Lactobacillus plantarum C7	570:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	1	19	theme	ethanol	370:376	arg1	EPS-ETOH					393:400	EPS-ETOH	393:400	EPS-ETOH	393:400	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	19	theme	ethanol	370:376	arg1	methods					361:367	two methods	357:367	two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF)	357:430	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	19	theme	ethanol	370:376	arg1	precipitation					378:390	ethanol precipitation	370:390	ethanol precipitation (EPS-ETOH)	370:401	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	2	20	theme	EPS	467:469	arg1	recovery					471:478	EPS recovery	467:478	EPS recovery by ultrafiltration	467:497	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	1	21	theme	cut-off	449:455	arg1	membrane					457:464	a 10 KDa cut-off membrane	440:464	a 10 KDa cut-off membrane	440:464	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	5	22	theme	Lactococcus	822:832	arg1	lactis					834:839	Lactococcus lactis SLT10	822:845	Lactococcus lactis SLT10	822:845	EPS isolated from Lactococcus lactis SLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF.
30320131	6	23	theme	molar	1089:1093	arg1	ratios					1095:1100	different molar ratios	1079:1100	different molar ratios	1079:1100	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	6	24	dep	composition	1041:1051	arg1	mannose					1066:1072	mannose	1066:1072	mannose	1066:1072	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	6	24	dep	composition	1041:1051	arg1	glucose					1054:1060	glucose	1054:1060	glucose	1054:1060	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	0	25	dep	Purification	81:92	arg1	the					77:79	the	77:79	the	77:79	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	4	26	theme	monomer	678:684	arg1	composition					686:696	The monomer composition	674:696	The monomer composition of the EPS fractions	674:717	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	2	27	theme	Lactococcus	541:551	arg1	lactis					553:558	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	2	27	theme	Lactococcus	541:551	arg1	SLT10					560:564	SLT10	560:564	SLT10	560:564	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	2	27	theme	Lactococcus	541:551	arg1	C7					594:595	Lactobacillus plantarum C7	570:595	Lactobacillus plantarum C7	570:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	1	28	theme	plantarum	289:297	arg1	C7					299:300	Lactobacillus plantarum C7	275:300	Lactobacillus plantarum C7	275:300	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	28	theme	plantarum	289:297	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	5	29	attach	isolated	808:815	arg1	lactis					834:839	Lactococcus lactis SLT10	822:845	Lactococcus lactis SLT10	822:845	EPS isolated from Lactococcus lactis SLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF.
30320131	5	29	attach	isolated	808:815	arg2	EPS					804:806	EPS	804:806	EPS isolated from Lactococcus lactis SLT10	804:845	EPS isolated from Lactococcus lactis SLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF.
30320131	7	30	theme	EPS	1142:1144	arg1	fractions					1146:1154	the different EPS fractions	1128:1154	the different EPS fractions	1128:1154	The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa.
30320131	0	31	theme	Efficiency	18:27	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.	0:174	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	8	32	theme	EPS-ETOH	1217:1224	arg1	fractions					1226:1234	EPS-ETOH fractions	1217:1234	EPS-ETOH fractions	1217:1234	Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions.
30320131	6	33	theme	Lactobacillus	966:978	arg1	C7					990:991	Lactobacillus plantarum C7	966:991	Lactobacillus plantarum C7	966:991	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	0	34	from	Purification	81:92	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.	0:174	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	4	35	theme	molar	758:762	arg1	ratios					764:769	molar ratios	758:769	molar ratios	758:769	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	0	36	theme	Ethanol	32:38	arg1	Efficiency					18:27	the Efficiency	14:27	the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria	14:173	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	9	37	theme	functional	1372:1381	arg1	C=O					1401:1403	C=O	1401:1403	C=O	1401:1403	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	37	theme	functional	1372:1381	arg1	groups					1383:1388	the functional groups	1368:1388	the functional groups (O-H, C-H, C=O, -COO, and C-O-C)	1368:1421	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	37	theme	functional	1372:1381	arg1	O-H					1391:1393	O-H	1391:1393	O-H	1391:1393	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	37	theme	functional	1372:1381	arg1	-COO					1406:1409	-COO	1406:1409	-COO	1406:1409	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	37	theme	functional	1372:1381	arg1	C-O-C					1416:1420	C-O-C	1416:1420	C-O-C	1416:1420	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	37	theme	functional	1372:1381	arg1	C-H					1396:1398	C-H	1396:1398	C-H	1396:1398	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	0	38	theme	Lactic	154:159	arg1	Bacteria					166:173	Three Lactic Acid Bacteria	148:173	Three Lactic Acid Bacteria	148:173	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	1	39	theme	Lactic	219:224	arg1	Bacteria					231:238	three Lactic Acid Bacteria	213:238	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	40	theme	Leuconostoc	307:317	arg1	B3					333:334	Leuconostoc mesenteroides B3	307:334	Leuconostoc mesenteroides B3	307:334	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	40	theme	Leuconostoc	307:317	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	8	41	theme	fractions	1226:1234	arg1	weights					1206:1212	Molecular weights	1196:1212	Molecular weights of EPS-ETOH fractions	1196:1234	Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions.
30320131	1	42	theme	Acid	226:229	arg1	Bacteria					231:238	three Lactic Acid Bacteria	213:238	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	7	43	theme	fractions	1146:1154	arg1	weights					1117:1123	The molecular weights	1103:1123	The molecular weights of the different EPS fractions	1103:1154	The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa.
30320131	1	44	theme	mesenteroides	319:331	arg1	B3					333:334	Leuconostoc mesenteroides B3	307:334	Leuconostoc mesenteroides B3	307:334	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	44	theme	mesenteroides	319:331	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	0	45	dep	Ethanol	32:38	arg1	Ultrafiltration					58:72	Ultrafiltration	58:72	Ultrafiltration	58:72	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	0	45	dep	Ethanol	32:38	arg1	Precipitation					40:52	Precipitation	40:52	Precipitation	40:52	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	9	46	dep	groups	1383:1388	arg1	C=O					1401:1403	C=O	1401:1403	C=O	1401:1403	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	46	dep	groups	1383:1388	arg1	groups					1383:1388	the functional groups	1368:1388	the functional groups (O-H, C-H, C=O, -COO, and C-O-C)	1368:1421	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	46	dep	groups	1383:1388	arg1	O-H					1391:1393	O-H	1391:1393	O-H	1391:1393	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	46	dep	groups	1383:1388	arg1	-COO					1406:1409	-COO	1406:1409	-COO	1406:1409	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	46	dep	groups	1383:1388	arg1	C-O-C					1416:1420	C-O-C	1416:1420	C-O-C	1416:1420	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	46	dep	groups	1383:1388	arg1	C-H					1396:1398	C-H	1396:1398	C-H	1396:1398	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	1	47	dep	methods	361:367	arg1	methods					361:367	two methods	357:367	two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF)	357:430	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	47	dep	methods	361:367	arg1	EPS-UF					424:429	EPS-UF	424:429	EPS-UF	424:429	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	47	dep	methods	361:367	arg1	EPS-ETOH					393:400	EPS-ETOH	393:400	EPS-ETOH	393:400	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	47	dep	methods	361:367	arg1	ultrafiltration					407:421	ultrafiltration	407:421	ultrafiltration (EPS-UF)	407:430	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	47	dep	methods	361:367	arg1	precipitation					378:390	ethanol precipitation	370:390	ethanol precipitation (EPS-ETOH)	370:401	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	0	48	theme	Acid	161:164	arg1	Bacteria					166:173	Three Lactic Acid Bacteria	148:173	Three Lactic Acid Bacteria	148:173	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	6	49	dep	Lactobacillus	966:978	arg1	plantarum					980:988	plantarum	980:988	plantarum	980:988	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	9	50	attach	isolated	1439:1446	arg1	strains					1463:1469	the three strains	1453:1469	the three strains	1453:1469	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	9	50	attach	isolated	1439:1446	arg2	EPS					1435:1437	the EPS	1431:1437	the EPS isolated from the three strains	1431:1469	Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
30320131	4	51	theme	fractions	709:717	arg1	composition					686:696	The monomer composition	674:696	The monomer composition of the EPS fractions	674:717	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	2	52	theme	Lactobacillus	570:582	arg1	lactis					553:558	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	Lactococcus lactis SLT10 and Lactobacillus plantarum C7	541:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	2	52	theme	Lactobacillus	570:582	arg1	C7					594:595	Lactobacillus plantarum C7	570:595	Lactobacillus plantarum C7	570:595	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	4	53	from	differences	728:738	arg1	structures					743:752	structures	743:752	structures	743:752	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	4	53	from	differences	728:738	arg1	ratios					764:769	molar ratios	758:769	molar ratios	758:769	The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
30320131	1	54	theme	Bacteria	231:238	arg1	B3					333:334	Leuconostoc mesenteroides B3	307:334	Leuconostoc mesenteroides B3	307:334	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	54	theme	Bacteria	231:238	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	54	theme	Bacteria	231:238	arg1	lactis					261:266	Lactococcus lactis SLT10	249:272	Lactococcus lactis SLT10	249:272	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	54	theme	Bacteria	231:238	arg1	C7					299:300	Lactobacillus plantarum C7	275:300	Lactobacillus plantarum C7	275:300	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	0	55	from	Characteristics	98:112	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.	0:174	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	2	56	dep	Lactobacillus	570:582	arg1	plantarum					584:592	plantarum	584:592	plantarum	584:592	EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
30320131	8	57	theme	Molecular	1196:1204	arg1	weights					1206:1212	Molecular weights	1196:1212	Molecular weights of EPS-ETOH fractions	1196:1234	Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions.
30320131	6	58	theme	similar	1033:1039	arg1	composition					1041:1051	similar composition	1033:1051	similar composition (glucose and mannose)	1033:1073	EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
30320131	3	59	with	similar	614:620	arg1	methods					632:638	both methods	627:638	both methods for Leuconostoc mesenteroides B3	627:671	However, it was similar with both methods for Leuconostoc mesenteroides B3.
30320131	0	60	from	Efficiency	18:27	arg1	Characteristics					98:112	Characteristics	98:112	Characteristics	98:112	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	0	60	from	Efficiency	18:27	arg1	Purification					81:92	Purification	81:92	Purification	81:92	Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria.
30320131	3	61	dep	mesenteroides	656:668	arg1	B3					670:671	B3	670:671	Leuconostoc mesenteroides B3	644:671	However, it was similar with both methods for Leuconostoc mesenteroides B3.
30320131	1	62	theme	Lactococcus	249:259	arg1	strains					240:246	three Lactic Acid Bacteria strains	213:246	three Lactic Acid Bacteria strains	213:246	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30320131	1	62	theme	Lactococcus	249:259	arg1	lactis					261:266	Lactococcus lactis SLT10	249:272	Lactococcus lactis SLT10	249:272	Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
30680910	0	0	theme	carboxymethyl	88:100	arg1	starch					102:107	sodium carboxymethyl starch	81:107	sodium carboxymethyl starch	81:107	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	7	1	theme	colour	1110:1115	arg1	QDs					1117:1119	the other colour QDs	1100:1119	the other colour QDs	1100:1119	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	4	2	theme	QDs-starch	542:551	arg1	composite					553:561	the QDs-starch composite	538:561	the QDs-starch composite	538:561	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	0	3	theme	sodium	81:86	arg1	starch					102:107	sodium carboxymethyl starch	81:107	sodium carboxymethyl starch	81:107	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	4	4	theme	Scanning	480:487	arg1	SEM					510:512	SEM	510:512	SEM	510:512	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	4	4	theme	Scanning	480:487	arg1	microscopy					498:507	Scanning electron microscopy	480:507	Scanning electron microscopy (SEM) observation	480:525	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	6	5	theme	Combined	762:769	arg1	lifetime					783:790	Combined fluorescent lifetime	762:790	Combined fluorescent lifetime	762:790	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	5	6	theme	Energy	632:637	arg1	EDX					670:672	EDX	670:672	EDX	670:672	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	5	6	theme	Energy	632:637	arg1	spectroscopy					656:667	Energy dispersive X-ray spectroscopy	632:667	Energy dispersive X-ray spectroscopy (EDX) results	632:681	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	7	7	contain	have	1135:1138	arg1	they					1121:1124	they	1121:1124	they	1121:1124	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	7	7	contain	have	1135:1138	arg2	QYs					1144:1146	the QYs	1140:1146	the QYs	1140:1146	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	5	8	theme	dispersive	639:648	arg1	EDX					670:672	EDX	670:672	EDX	670:672	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	5	8	theme	dispersive	639:648	arg1	spectroscopy					656:667	Energy dispersive X-ray spectroscopy	632:667	Energy dispersive X-ray spectroscopy (EDX) results	632:681	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	2	9	theme	carboxymethyl	265:277	arg1	CMS-Na					287:292	CMS-Na	287:292	CMS-Na	287:292	Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite.
30680910	2	9	theme	carboxymethyl	265:277	arg1	starch					279:284	Sodium carboxymethyl starch	258:284	Sodium carboxymethyl starch (CMS-Na)	258:293	Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite.
30680910	9	10	theme	QD	1368:1369	arg1	composites					1371:1380	high QY QD composites	1360:1380	high QY QD composites	1360:1380	These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
30680910	5	11	theme	intensive	703:711	arg1	Te					724:725	Te	724:725	Te	724:725	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	5	11	theme	intensive	703:711	arg1	tellurium					713:721	intensive tellurium	703:721	intensive tellurium (Te)	703:726	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	7	12	theme	colour	1026:1031	arg1	QDs					1048:1050	the red colour (λem = 660 nm) QDs	1018:1050	the red colour (λem = 660 nm) QDs	1018:1050	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	5	13	theme	element	745:751	arg1	signals					753:759	element signals	745:759	element signals	745:759	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	2	14	theme	Sodium	258:263	arg1	CMS-Na					287:292	CMS-Na	287:292	CMS-Na	287:292	Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite.
30680910	2	14	theme	Sodium	258:263	arg1	starch					279:284	Sodium carboxymethyl starch	258:284	Sodium carboxymethyl starch (CMS-Na)	258:293	Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite.
30680910	1	15	theme	luminescent	172:182	arg1	dots					192:195	well-dispersed and highly luminescent quantum dots	146:195	well-dispersed and highly luminescent quantum dots (QDs) powder	146:208	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	1	15	theme	luminescent	172:182	arg1	QDs					198:200	QDs	198:200	QDs	198:200	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	6	16	theme	quantum	847:853	arg1	yields					855:860	the QDs-starch quantum yields	832:860	the QDs-starch quantum yields (QYs)	832:866	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	6	16	theme	quantum	847:853	arg1	QYs					863:865	QYs	863:865	QYs	863:865	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	8	17	theme	fluorescent	1214:1224	arg1	composite					1237:1245	the fluorescent QDs-starch composite	1210:1245	the fluorescent QDs-starch composite	1210:1245	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	1	18	theme	quantum	184:190	arg1	dots					192:195	well-dispersed and highly luminescent quantum dots	146:195	well-dispersed and highly luminescent quantum dots (QDs) powder	146:208	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	1	18	theme	quantum	184:190	arg1	QDs					198:200	QDs	198:200	QDs	198:200	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	3	19	theme	Ultraviolet	358:368	arg1	light					375:379	Ultraviolet (UV) light	358:379	Ultraviolet (UV) light	358:379	Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent.
30680910	6	20	theme	QDs-starch	836:845	arg1	yields					855:860	the QDs-starch quantum yields	832:860	the QDs-starch quantum yields (QYs)	832:866	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	6	20	theme	QDs-starch	836:845	arg1	QYs					863:865	QYs	863:865	QYs	863:865	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	5	21	dep	tellurium	713:721	arg1	signals					753:759	element signals	745:759	element signals	745:759	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	1	22	theme	dots	192:195	arg1	powder					203:208	well-dispersed and highly luminescent quantum dots (QDs) powder	146:208	well-dispersed and highly luminescent quantum dots (QDs) powder	146:208	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	0	23	theme	High	0:3	arg1	yield					13:17	High quantum yield	0:17	High quantum yield	0:17	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	7	24	theme	red	1022:1024	arg1	colour					1026:1031	the red colour	1018:1031	the red colour (λem = 660 nm) QDs	1018:1050	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	7	24	theme	red	1022:1024	arg1	= 660 nm					1038:1045	λem = 660 nm	1034:1045	λem = 660 nm	1034:1045	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	6	25	theme	mol/g	922:926	arg1	mol/g					940:944	1 × 10-6 mol/g to 2 × 10-6 mol/g	913:944	1 × 10-6 mol/g to 2 × 10-6 mol/g	913:944	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	8	26	theme	half-decay	1253:1262	arg1	time					1264:1267	a half-decay time	1251:1267	a half-decay time of 40.23 s	1251:1278	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	9	27	theme	promising	1325:1333	arg1	dispersant					1339:1348	a promising QDs dispersant	1323:1348	a promising QDs dispersant to obtain high QY QD composites	1323:1380	These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
30680910	9	27	theme	promising	1325:1333	arg1	CMS-Na					1313:1318	the CMS-Na	1309:1318	the CMS-Na	1309:1318	These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
30680910	8	28	theme	QDs-starch	1226:1235	arg1	composite					1237:1245	the fluorescent QDs-starch composite	1210:1245	the fluorescent QDs-starch composite	1210:1245	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	8	29	theme	photobleaching	1173:1186	arg1	experiments					1188:1198	Time-resolved photobleaching experiments	1159:1198	Time-resolved photobleaching experiments	1159:1198	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	0	30	theme	quantum	38:44	arg1	dots					46:49	well-dispersed quantum dots	23:49	well-dispersed quantum dots	23:49	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	9	31	theme	high	1360:1363	arg1	composites					1371:1380	high QY QD composites	1360:1380	high QY QD composites	1360:1380	These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
30680910	3	32	contain	had	411:413	arg1	starches					402:409	the blank starches	392:409	the blank starches	392:409	Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent.
30680910	3	32	contain	had	411:413	arg2	fluorescence					418:429	no fluorescence	415:429	no fluorescence	415:429	Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent.
30680910	4	33	with	morphology	588:597	arg1	diameter					608:615	the diameter	604:615	the diameter around 200 nm	604:629	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	0	34	theme	well-dispersed	23:36	arg1	dots					46:49	well-dispersed quantum dots	23:49	well-dispersed quantum dots	23:49	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	4	35	theme	microscopy	498:507	arg1	observation					515:525	Scanning electron microscopy (SEM) observation	480:525	Scanning electron microscopy (SEM) observation	480:525	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	4	36	contain	has	563:565	arg1	composite					553:561	the QDs-starch composite	538:561	the QDs-starch composite	538:561	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	4	36	contain	has	563:565	arg2	morphology					588:597	the typical particle morphology	567:597	the typical particle morphology with the diameter around 200 nm	567:629	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	3	37	theme	UV	371:372	arg1	light					375:379	Ultraviolet (UV) light	358:379	Ultraviolet (UV) light	358:379	Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent.
30680910	4	38	theme	electron	489:496	arg1	SEM					510:512	SEM	510:512	SEM	510:512	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	4	38	theme	electron	489:496	arg1	microscopy					498:507	Scanning electron microscopy	480:507	Scanning electron microscopy (SEM) observation	480:525	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	5	39	theme	X-ray	650:654	arg1	EDX					670:672	EDX	670:672	EDX	670:672	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	5	39	theme	X-ray	650:654	arg1	spectroscopy					656:667	Energy dispersive X-ray spectroscopy	632:667	Energy dispersive X-ray spectroscopy (EDX) results	632:681	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	3	40	theme	blank	396:400	arg1	starches					402:409	the blank starches	392:409	the blank starches	392:409	Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent.
30680910	8	41	theme	Time-resolved	1159:1171	arg1	experiments					1188:1198	Time-resolved photobleaching experiments	1159:1198	Time-resolved photobleaching experiments	1159:1198	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	5	42	theme	spectroscopy	656:667	arg1	results					675:681	Energy dispersive X-ray spectroscopy (EDX) results	632:681	Energy dispersive X-ray spectroscopy (EDX) results	632:681	Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals.
30680910	6	43	theme	steady-state	796:807	arg1	spectrometer					809:820	steady-state spectrometer	796:820	steady-state spectrometer	796:820	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	8	44	theme	40.23 s	1272:1278	arg1	time					1264:1267	a half-decay time	1251:1267	a half-decay time of 40.23 s	1251:1278	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	1	45	theme	challenging	118:128	arg1	task					130:133	a challenging task	116:133	a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder	116:208	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	1	45	theme	challenging	118:128	arg1	It					110:111	It	110:111	It	110:111	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	9	46	theme	QY	1365:1366	arg1	composites					1371:1380	high QY QD composites	1360:1380	high QY QD composites	1360:1380	These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
30680910	4	47	theme	typical	571:577	arg1	morphology					588:597	the typical particle morphology	567:597	the typical particle morphology with the diameter around 200 nm	567:629	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
30680910	7	48	theme	λem	1034:1036	arg1	colour					1026:1031	the red colour	1018:1031	the red colour (λem = 660 nm) QDs	1018:1050	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	7	48	theme	λem	1034:1036	arg1	= 660 nm					1038:1045	λem = 660 nm	1034:1045	λem = 660 nm	1034:1045	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	1	49	theme	well-dispersed	146:159	arg1	dots					192:195	well-dispersed and highly luminescent quantum dots	146:195	well-dispersed and highly luminescent quantum dots (QDs) powder	146:208	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	1	49	theme	well-dispersed	146:159	arg1	QDs					198:200	QDs	198:200	QDs	198:200	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	1	50	theme	new	216:218	arg1	strategy					220:227	a new strategy	214:227	a new strategy	214:227	It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article.
30680910	6	51	theme	fluorescent	771:781	arg1	lifetime					783:790	Combined fluorescent lifetime	762:790	Combined fluorescent lifetime	762:790	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	8	52	contain	has	1247:1249	arg2	time					1264:1267	a half-decay time	1251:1267	a half-decay time of 40.23 s	1251:1278	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	8	52	contain	has	1247:1249	arg1	composite					1237:1245	the fluorescent QDs-starch composite	1210:1245	the fluorescent QDs-starch composite	1210:1245	Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s.
30680910	2	53	theme	QDs-starch	336:345	arg1	composite					347:355	the QDs-starch composite	332:355	the QDs-starch composite	332:355	Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite.
30680910	7	54	theme	other	1104:1108	arg1	QDs					1117:1119	the other colour QDs	1100:1119	the other colour QDs	1100:1119	For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%.
30680910	6	55	dep	mol/g	940:944	arg1	to					928:929	to	928:929	to	928:929	Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly.
30680910	0	56	theme	quantum	5:11	arg1	yield					13:17	High quantum yield	0:17	High quantum yield	0:17	High quantum yield and well-dispersed quantum dots luminescent composite through sodium carboxymethyl starch.
30680910	4	57	theme	particle	579:586	arg1	morphology					588:597	the typical particle morphology	567:597	the typical particle morphology with the diameter around 200 nm	567:629	Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm.
31622174	0	0	theme	Tyromyces	83:91	arg1	floriformis					93:103	the Basidiomycete Tyromyces floriformis	65:103	the Basidiomycete Tyromyces floriformis	65:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	5	1	theme	building	770:777	arg1	blocks					779:784	the building blocks	766:784	the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	766:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	9	2	theme	acetyl	1443:1448	arg1	donors					1450:1455	acetyl donors	1443:1455	acetyl donors	1443:1455	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	1	3	theme	potent	157:162	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	3	theme	potent	157:162	arg1	producer					185:192	a potent fungal sesquiterpene producer	155:192	a potent fungal sesquiterpene producer	155:192	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	0	4	theme	Basidiomycete	69:81	arg1	floriformis					93:103	the Basidiomycete Tyromyces floriformis	65:103	the Basidiomycete Tyromyces floriformis	65:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	8	5	theme	Postia	1297:1302	arg1	placenta					1304:1311	Postia placenta	1297:1311	Postia placenta	1297:1311	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	10	6	theme	insect-attracting	1730:1746	arg1	sesquiterpenes					1748:1761	the possibly insect-attracting sesquiterpenes	1717:1761	the possibly insect-attracting sesquiterpenes	1717:1761	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	1	7	theme	fungal	164:169	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	7	theme	fungal	164:169	arg1	producer					185:192	a potent fungal sesquiterpene producer	155:192	a potent fungal sesquiterpene producer	155:192	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	8	theme	submerged	246:254	arg1	culture					256:262	submerged culture	246:262	submerged culture	246:262	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	6	9	theme	>40	1094:1096	arg1	mg					1098:1099	mg	1098:1099	mg	1098:1099	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	0	10	from	Response	0:7	arg1	cultures					53:60	submerged cultures	43:60	submerged cultures of the Basidiomycete Tyromyces floriformis	43:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	3	11	theme	qualitative	543:553	arg1	spectrum					563:570	the qualitative product spectrum	539:570	the qualitative product spectrum	539:570	Additives, such as some polysaccharides or lipids, did not affect the qualitative product spectrum but strongly affected the quantitative synthesis.
31622174	0	12	theme	floriformis	93:103	arg1	cultures					53:60	submerged cultures	43:60	submerged cultures of the Basidiomycete Tyromyces floriformis	43:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	0	13	from	cultures	53:60	arg1	Response					0:7	Response	0:7	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.	0:130	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	8	14	theme	fungal	1373:1378	arg1	synthesis					1396:1404	fungal sesquiterpenoid synthesis	1373:1404	fungal sesquiterpenoid synthesis	1373:1404	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	3	15	theme	product	555:561	arg1	spectrum					563:570	the qualitative product spectrum	539:570	the qualitative product spectrum	539:570	Additives, such as some polysaccharides or lipids, did not affect the qualitative product spectrum but strongly affected the quantitative synthesis.
31622174	5	16	theme	inhibitory	848:857	arg1	effect					859:864	no inhibitory effect	845:864	no inhibitory effect	845:864	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	0	17	theme	medium	112:117	arg1	composition					119:129	the medium composition	108:129	the medium composition	108:129	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	9	18	theme	donors	1450:1455	arg1	addition					1431:1438	the addition	1427:1438	the addition of acetyl donors	1427:1455	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	5	19	theme	acid	833:836	arg1	blocks					779:784	the building blocks	766:784	the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	766:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	2	20	theme	fruity	433:438	arg1	α-ylangene					440:449	the fruity α-ylangene	429:449	them the fruity α-ylangene as the main volatile	424:470	Thirty-one sesquiterpenes and sesquiterpenoids were identified in the supernatant, among them the fruity α-ylangene as the main volatile.
31622174	3	21	dep	polysaccharides	497:511	arg1	some					492:495	some	492:495	some	492:495	Additives, such as some polysaccharides or lipids, did not affect the qualitative product spectrum but strongly affected the quantitative synthesis.
31622174	1	22	theme	sesquiterpene	171:183	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	22	theme	sesquiterpene	171:183	arg1	producer					185:192	a potent fungal sesquiterpene producer	155:192	a potent fungal sesquiterpene producer	155:192	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	5	23	theme	arabinose	803:811	arg1	blocks					779:784	the building blocks	766:784	the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	766:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	4	24	theme	Rye	622:624	arg1	arabinoxylan					626:637	Rye arabinoxylan	622:637	Rye arabinoxylan	622:637	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	5	25	theme	Single	747:752	arg1	addition					754:761	Single addition	747:761	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	747:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	0	26	from	synthesis	30:38	arg1	cultures					53:60	submerged cultures	43:60	submerged cultures of the Basidiomycete Tyromyces floriformis	43:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	10	27	theme	symbiotic	1639:1647	arg1	lifestyle					1649:1657	a symbiotic lifestyle	1637:1657	a symbiotic lifestyle with wasps	1637:1668	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	10	28	theme	higher	1692:1697	arg1	concentrations					1699:1712	higher concentrations	1692:1712	higher concentrations of the possibly insect-attracting sesquiterpenes	1692:1761	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	2	29	theme	main	458:461	arg1	volatile					463:470	the main volatile	454:470	the main volatile	454:470	Thirty-one sesquiterpenes and sesquiterpenoids were identified in the supernatant, among them the fruity α-ylangene as the main volatile.
31622174	7	30	theme	α-ylangene	1142:1151	arg1	concentrations					1153:1166	α-ylangene concentrations	1142:1166	α-ylangene concentrations	1142:1166	Both increased as well as decreased α-ylangene concentrations correlated with the intracellular sesquiterpene cyclase activity.
31622174	8	31	theme	cultured	1270:1277	arg1	unicolor					1287:1294	submerged cultured Cerrena unicolor	1260:1294	submerged cultured Cerrena unicolor	1260:1294	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	4	32	theme	α-ylangene	735:744	arg1	synthesis					722:730	the synthesis	718:730	the synthesis of α-ylangene	718:744	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	8	33	theme	Cerrena	1279:1285	arg1	unicolor					1287:1294	submerged cultured Cerrena unicolor	1260:1294	submerged cultured Cerrena unicolor	1260:1294	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	0	34	theme	sesquiterpene	16:28	arg1	synthesis					30:38	the sesquiterpene synthesis	12:38	the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis	12:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	5	35	theme	xylose	814:819	arg1	blocks					779:784	the building blocks	766:784	the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	766:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	9	36	theme	biphasic	1486:1493	arg1	cultures					1495:1502	all biphasic cultures	1482:1502	all biphasic cultures	1482:1502	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	5	37	theme	ferulic	825:831	arg1	acid					833:836	ferulic acid	825:836	ferulic acid	825:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	6	38	theme	donors	1020:1025	arg1	addition					1001:1008	addition	1001:1008	addition of acetyl donors	1001:1025	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	5	39	dep	%	879:879	arg1	w/v					882:884	w/v	882:884	w/v	882:884	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	1	40	theme	Cerrena	205:211	arg1	organism					234:241	a model organism	226:241	a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro	226:332	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	40	theme	Cerrena	205:211	arg1	unicolor					213:220	Cerrena unicolor	205:220	Cerrena unicolor	205:220	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	40	theme	Cerrena	205:211	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	8	41	theme	Similar	1234:1240	arg1	experiments					1242:1252	Similar experiments	1234:1252	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea	1234:1336	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	7	42	theme	sesquiterpene	1202:1214	arg1	activity					1224:1231	the intracellular sesquiterpene cyclase activity	1184:1231	the intracellular sesquiterpene cyclase activity	1184:1231	Both increased as well as decreased α-ylangene concentrations correlated with the intracellular sesquiterpene cyclase activity.
31622174	10	43	theme	sesquiterpenes	1748:1761	arg1	concentrations					1699:1712	higher concentrations	1692:1712	higher concentrations of the possibly insect-attracting sesquiterpenes	1692:1761	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	0	44	theme	synthesis	30:38	arg1	Response					0:7	Response	0:7	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.	0:130	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	1	45	theme	sesquiterpene	298:310	arg1	biosynthesis					312:323	sesquiterpene biosynthesis	298:323	sesquiterpene biosynthesis	298:323	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	5	46	theme	blocks	779:784	arg1	addition					754:761	Single addition	747:761	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	747:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	2	47	dep	them	424:427	arg1	α-ylangene					440:449	the fruity α-ylangene	429:449	them the fruity α-ylangene as the main volatile	424:470	Thirty-one sesquiterpenes and sesquiterpenoids were identified in the supernatant, among them the fruity α-ylangene as the main volatile.
31622174	6	48	theme	α-ylangene	1039:1048	arg1	concentration					1050:1062	the α-ylangene concentration	1035:1062	the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1	1035:1103	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	7	49	theme	cyclase	1216:1222	arg1	activity					1224:1231	the intracellular sesquiterpene cyclase activity	1184:1231	the intracellular sesquiterpene cyclase activity	1184:1231	Both increased as well as decreased α-ylangene concentrations correlated with the intracellular sesquiterpene cyclase activity.
31622174	8	50	theme	sesquiterpenoid	1380:1394	arg1	synthesis					1396:1404	fungal sesquiterpenoid synthesis	1373:1404	fungal sesquiterpenoid synthesis	1373:1404	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	6	51	dep	L-1	1101:1103	arg1	up					1088:1089	up	1088:1089	up	1088:1089	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	9	52	from	polysaccharides	1525:1539	arg1	fungi					1544:1548	fungi	1544:1548	fungi	1544:1548	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	8	53	theme	Coprinopsis	1318:1328	arg1	cinerea					1330:1336	Coprinopsis cinerea	1318:1336	Coprinopsis cinerea	1318:1336	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	0	54	theme	submerged	43:51	arg1	cultures					53:60	submerged cultures	43:60	submerged cultures of the Basidiomycete Tyromyces floriformis	43:103	Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition.
31622174	1	55	from	organism	234:241	arg1	culture					256:262	submerged culture	246:262	submerged culture	246:262	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	9	56	from	synthesis	1469:1477	arg1	cultures					1495:1502	all biphasic cultures	1482:1502	all biphasic cultures	1482:1502	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	3	57	theme	quantitative	598:609	arg1	synthesis					611:619	the quantitative synthesis	594:619	the quantitative synthesis	594:619	Additives, such as some polysaccharides or lipids, did not affect the qualitative product spectrum but strongly affected the quantitative synthesis.
31622174	9	58	theme	lignified	1578:1586	arg1	plants					1588:1593	lignified plants	1578:1593	lignified plants	1578:1593	Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants.
31622174	1	59	theme	Tyromyces	132:140	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	59	theme	Tyromyces	132:140	arg1	unicolor					213:220	Cerrena unicolor	205:220	Cerrena unicolor	205:220	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	59	theme	Tyromyces	132:140	arg1	producer					185:192	a potent fungal sesquiterpene producer	155:192	a potent fungal sesquiterpene producer	155:192	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	59	theme	Tyromyces	132:140	arg1	organism					234:241	a model organism	226:241	a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro	226:332	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	10	60	with	lifestyle	1649:1657	arg1	wasps					1664:1668	wasps	1664:1668	wasps	1664:1668	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	8	61	theme	submerged	1260:1268	arg1	unicolor					1287:1294	submerged cultured Cerrena unicolor	1260:1294	submerged cultured Cerrena unicolor	1260:1294	Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently.
31622174	5	62	theme	significant	963:973	arg1	suppression					975:985	a significant suppression	961:985	a significant suppression	961:985	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	5	63	theme	larger	925:930	arg1	oligosaccharides					932:947	larger oligosaccharides	925:947	larger oligosaccharides	925:947	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	2	64	theme	Thirty-one	335:344	arg1	sesquiterpenes					346:359	Thirty-one sesquiterpenes	335:359	Thirty-one sesquiterpenes	335:359	Thirty-one sesquiterpenes and sesquiterpenoids were identified in the supernatant, among them the fruity α-ylangene as the main volatile.
31622174	10	65	theme	Cerrena	1609:1615	arg1	unicolor					1617:1624	Cerrena unicolor	1609:1624	Cerrena unicolor	1609:1624	In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
31622174	6	66	theme	mg	1098:1099	arg1	L-1					1101:1103	>40 mg L-1	1094:1103	>40 mg L-1	1094:1103	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	4	67	theme	other	643:647	arg1	polysaccharides					649:663	other polysaccharides	643:663	other polysaccharides	643:663	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	4	67	theme	other	643:647	arg1	starch					682:687	starch	682:687	starch	682:687	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	4	67	theme	other	643:647	arg1	agarose					694:700	agarose	694:700	agarose	694:700	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	4	67	theme	other	643:647	arg1	chitin					674:679	chitin	674:679	chitin	674:679	Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of α-ylangene.
31622174	5	68	theme	0.05	875:878	arg1	%					879:879	%	879:879	%	879:879	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	6	69	theme	magnitude	1078:1086	arg1	order					1069:1073	1 order	1067:1073	1 order of magnitude	1067:1086	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31622174	1	70	theme	model	228:232	arg1	organism					234:241	a model organism	226:241	a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro	226:332	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	70	theme	model	228:232	arg1	unicolor					213:220	Cerrena unicolor	205:220	Cerrena unicolor	205:220	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	1	70	theme	model	228:232	arg1	floriformis					142:152	Tyromyces floriformis	132:152	Tyromyces floriformis	132:152	Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro.
31622174	7	71	theme	intracellular	1188:1200	arg1	activity					1224:1231	the intracellular sesquiterpene cyclase activity	1184:1231	the intracellular sesquiterpene cyclase activity	1184:1231	Both increased as well as decreased α-ylangene concentrations correlated with the intracellular sesquiterpene cyclase activity.
31622174	5	72	theme	arabinoxylan	789:800	arg1	blocks					779:784	the building blocks	766:784	the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid	766:836	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	5	73	theme	%	879:879	arg1	32-α-l-arabinofuranosyl-xylobiose					887:919	0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose	875:919	0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose	875:919	Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 32-α-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression.
31622174	6	74	theme	acetyl	1013:1018	arg1	donors					1020:1025	acetyl donors	1013:1025	acetyl donors	1013:1025	In contrast, addition of acetyl donors boosted the α-ylangene concentration by 1 order of magnitude up to >40 mg L-1.
31219358	5	0	theme	final	692:696	arg1	material					698:705	the final material	688:705	the final material	688:705	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	5	1	theme	hemicellulose	671:683	arg1	hemicellulose					671:683	hemicellulose	671:683	hemicellulose	671:683	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	5	1	theme	hemicellulose	671:683	arg1	amount					661:666	the amount	657:666	the amount of hemicellulose in the final material	657:705	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	4	2	theme	ecological	507:516	arg1	material					518:525	an ecological material	504:525	an ecological material suitable for packaging purposes	504:557	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	0	3	theme	Opuntia	64:70	arg1	Mill					90:93	Opuntia ficus indica (L.) Mill	64:93	Opuntia ficus indica (L.) Mill	64:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	5	4	theme	simple	591:596	arg1	able					640:643	able	640:643	able	640:643	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	5	4	theme	simple	591:596	arg1	protocol					598:605	the simple protocol	587:605	the simple protocol based on hot water treatment	587:634	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	4	5	theme	fibrous	433:439	arg1	material					441:448	The fibrous material	429:448	The fibrous material	429:448	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	2	6	theme	large	235:239	arg1	waste					257:261	a large agro industrial waste	233:261	a large agro industrial waste in our territory	233:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	2	7	from	waste	257:261	arg1	territory					270:278	our territory	266:278	our territory	266:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	4	8	theme	suitable	527:534	arg1	material					518:525	an ecological material	504:525	an ecological material suitable for packaging purposes	504:557	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	0	9	theme	indica	78:83	arg1	Mill					90:93	Opuntia ficus indica (L.) Mill	64:93	Opuntia ficus indica (L.) Mill	64:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	2	10	from	cladodes	175:182	arg1	procedures					155:164	green procedures	149:164	green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory	149:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	0	11	theme	L.	86:87	arg1	Mill					90:93	Opuntia ficus indica (L.) Mill	64:93	Opuntia ficus indica (L.) Mill	64:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	2	12	theme	Cellulosic	109:118	arg1	fibres					120:125	Cellulosic fibres	109:125	Cellulosic fibres	109:125	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	3	13	theme	relative	322:329	arg1	composition					331:341	its relative composition	318:341	its relative composition	318:341	The materials have been analysed for its relative composition, applying, IR and TG methodologies and it was characterised by the absence of lignin.
31219358	0	14	theme	Hand-made	0:8	arg1	paper					10:14	Hand-made paper	0:14	Hand-made paper	0:14	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	4	15	theme	handmade	479:486	arg1	paper					488:492	a handmade paper	477:492	a handmade paper obtaining an ecological material suitable for packaging purposes	477:557	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	0	16	dep	Opuntia	64:70	arg1	ficus					72:76	ficus	72:76	ficus	72:76	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	3	17	theme	TG	361:362	arg1	methodologies					364:376	TG methodologies	361:376	TG methodologies	361:376	The materials have been analysed for its relative composition, applying, IR and TG methodologies and it was characterised by the absence of lignin.
31219358	2	18	theme	industrial	246:255	arg1	waste					257:261	a large agro industrial waste	233:261	a large agro industrial waste in our territory	233:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	0	19	theme	Mill	90:93	arg1	waste					55:59	cladode waste	47:59	cladode waste of Opuntia ficus indica (L.) Mill	47:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	2	20	theme	green	149:153	arg1	procedures					155:164	green procedures	149:164	green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory	149:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	2	21	theme	agro	241:244	arg1	waste					257:261	a large agro industrial waste	233:261	a large agro industrial waste in our territory	233:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	4	22	theme	paper	488:492	arg1	manufacture					462:472	the manufacture	458:472	the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes	458:557	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	0	23	theme	green	28:32	arg1	procedure					34:42	green procedure	28:42	green procedure of cladode waste of Opuntia ficus indica (L.) Mill	28:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	2	24	theme	ficus	195:199	arg1	cladodes					175:182	the cladodes	171:182	the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory	171:278	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	2	25	dep	ficus	195:199	arg1	L.					209:210	L.	209:210	L.	209:210	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	2	25	dep	ficus	195:199	arg1	indica					201:206	indica	201:206	Opuntia ficus indica (L.) Mill.	187:217	Cellulosic fibres have been obtained by green procedures from the cladodes of Opuntia ficus indica (L.) Mill., constituting a large agro industrial waste in our territory.
31219358	0	26	theme	cladode	47:53	arg1	waste					55:59	cladode waste	47:59	cladode waste of Opuntia ficus indica (L.) Mill	47:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
31219358	5	27	from	amount	661:666	arg1	material					698:705	the final material	688:705	the final material	688:705	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	5	28	theme	hot	616:618	arg1	treatment					626:634	hot water treatment	616:634	hot water treatment	616:634	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	3	29	theme	lignin	421:426	arg1	absence					410:416	the absence	406:416	the absence of lignin	406:426	The materials have been analysed for its relative composition, applying, IR and TG methodologies and it was characterised by the absence of lignin.
31219358	5	30	theme	water	620:624	arg1	treatment					626:634	hot water treatment	616:634	hot water treatment	616:634	The authors evidenced that the simple protocol based on hot water treatment was able to decrease the amount of hemicellulose in the final material.
31219358	4	31	theme	packaging	540:548	arg1	purposes					550:557	packaging purposes	540:557	packaging purposes	540:557	The fibrous material allowed the manufacture of a handmade paper obtaining an ecological material suitable for packaging purposes.
31219358	0	32	theme	waste	55:59	arg1	procedure					34:42	green procedure	28:42	green procedure of cladode waste of Opuntia ficus indica (L.) Mill	28:93	Hand-made paper obtained by green procedure of cladode waste of Opuntia ficus indica (L.) Mill.
29856921	11	0	contain	containing	2086:2095	arg2	composites					2139:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	11	0	contain	containing	2086:2095	arg1	blends					2079:2084	the adhesive blends	2066:2084	the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites	2066:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	10	1	dep	shape	1862:1866	arg1	inherent					1893:1900	inherent	1893:1900	inherent	1893:1900	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	7	2	theme	surface	1290:1296	arg1	morphologies					1298:1309	their particle surface morphologies	1275:1309	their particle surface morphologies	1275:1309	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	4	3	with	lactose	686:692	arg1	compositions					716:727	varying anomeric compositions	699:727	varying anomeric compositions	699:727	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	4	3	with	lactose	686:692	arg1	properties					745:754	physical properties	736:754	physical properties	736:754	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	2	4	theme	new	354:356	arg1	carriers					358:365	new carriers	354:365	new carriers	354:365	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	9	5	theme	spherical	1726:1734	arg1	particles					1736:1744	predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles	1661:1744	predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm	1661:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	10	6	theme	DPI	1983:1985	arg1	delivery					1987:1994	DPI delivery	1983:1994	DPI delivery	1983:1994	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	2	7	theme	powder	282:287	arg1	development					297:307	dry powder inhaler development	278:307	dry powder inhaler development	278:307	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	4	8	theme	lactose	686:692	arg1	grades					676:681	other grades	670:681	other grades of lactose with varying anomeric compositions and/or physical properties	670:754	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	4	9	theme	resulting	632:640	arg1	particles					642:650	the resulting particles	628:650	the resulting particles	628:650	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	11	10	theme	inferior	2042:2049	arg1	performance					2051:2061	the relatively inferior performance	2027:2061	the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites	2027:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	1	11	theme	administration	166:179	arg1	landscape					181:189	The pulmonary administration landscape	152:189	The pulmonary administration landscape	152:189	The pulmonary administration landscape has rapidly advanced in recent years.
29856921	9	12	theme	mean	1753:1756	arg1	size					1758:1761	a mean size	1751:1761	a mean size of 50.9 ± 0.4 μm	1751:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	7	13	theme	lactose	1138:1144	arg1	grades					1128:1133	The distinct selected grades	1106:1133	The distinct selected grades of lactose	1106:1144	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	11	14	theme	spray-dried	2111:2121	arg1	composites					2139:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	0	15	theme	Compositions	103:114	arg1	Properties					129:138	Physical Properties	120:138	Physical Properties of Lactose	120:149	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	0	15	theme	Compositions	103:114	arg1	Study					77:81	A Comparative Case Study	58:81	A Comparative Case Study of Diverse Anomeric Compositions	58:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	11	16	theme	adhesive	2070:2077	arg1	blends					2079:2084	the adhesive blends	2066:2084	the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites	2066:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	5	17	theme	variable	830:837	arg1	compositions					839:850	variable compositions	830:850	variable compositions	830:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	9	18	theme	±	1771:1771	arg1	μm					1777:1778	50.9 ± 0.4 μm	1766:1778	50.9 ± 0.4 μm	1766:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	7	19	theme	distinct	1110:1117	arg1	grades					1128:1133	The distinct selected grades	1106:1133	The distinct selected grades of lactose	1106:1144	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	2	20	theme	Targeted	229:236	arg1	design					238:243	Targeted design	229:243	Targeted design of particles by spray-drying for dry powder inhaler development	229:307	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	1	21	theme	recent	215:220	arg1	years					222:226	recent years	215:226	recent years	215:226	The pulmonary administration landscape has rapidly advanced in recent years.
29856921	9	22	theme	μm	1777:1778	arg1	size					1758:1761	a mean size	1751:1761	a mean size of 50.9 ± 0.4 μm	1751:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	8	23	theme	Adhesive	1360:1367	arg1	mixtures					1369:1376	Adhesive mixtures	1360:1376	Adhesive mixtures	1360:1376	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	4	24	theme	varying	699:705	arg1	compositions					716:727	varying anomeric compositions	699:727	varying anomeric compositions	699:727	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	8	25	theme	model	1458:1462	arg1	drug					1464:1467	a model drug	1456:1467	a model drug	1456:1467	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	8	26	theme	aerodynamic	1543:1553	arg1	performance					1555:1565	aerodynamic performance	1543:1565	aerodynamic performance	1543:1565	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	8	27	theme	different	1385:1393	arg1	carriers					1403:1410	the different lactose carriers	1381:1410	the different lactose carriers with inhalable-sized salbutamol sulfate	1381:1450	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	7	28	theme	particle	1281:1288	arg1	morphologies					1298:1309	their particle surface morphologies	1275:1309	their particle surface morphologies	1275:1309	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	5	29	from	lactose	785:791	arg1	solutions					817:825	lactose-PEG 200 feed solutions	796:825	lactose-PEG 200 feed solutions of variable compositions	796:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	6	30	theme	differential	1039:1050	arg1	calorimetry					1061:1071	modulated differential scanning calorimetry	1029:1071	modulated differential scanning calorimetry	1029:1071	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	4	31	theme	integrated	497:506	arg1	approach					508:515	an integrated approach	494:515	an integrated approach	494:515	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	8	32	theme	carriers	1403:1410	arg1	mixtures					1369:1376	Adhesive mixtures	1360:1376	Adhesive mixtures	1360:1376	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	0	33	theme	Comparative	60:70	arg1	Study					77:81	A Comparative Case Study	58:81	A Comparative Case Study of Diverse Anomeric Compositions	58:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	5	34	from	composition	770:780	arg1	solutions					817:825	lactose-PEG 200 feed solutions	796:825	lactose-PEG 200 feed solutions of variable compositions	796:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	8	35	theme	Next	1575:1578	arg1	Impactor					1591:1598	a Next Generation Impactor	1573:1598	a Next Generation Impactor	1573:1598	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	8	36	theme	inhalable-sized	1417:1431	arg1	sulfate					1444:1450	inhalable-sized salbutamol sulfate	1417:1450	inhalable-sized salbutamol sulfate	1417:1450	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	9	37	theme	spray-dried	1625:1635	arg1	particles					1637:1645	the spray-dried particles	1621:1645	the spray-dried particles	1621:1645	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	6	38	theme	spray-dried	1001:1011	arg1	particles					1013:1021	the resulting spray-dried particles	987:1021	the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering	987:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	0	39	theme	Diverse	86:92	arg1	Compositions					103:114	Diverse Anomeric Compositions	86:114	Diverse Anomeric Compositions	86:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	10	40	dep	control	1975:1981	arg1	micromeritic					1829:1840	micromeritic	1829:1840	micromeritic	1829:1840	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	0	41	theme	Assessment	0:9	arg1	Design					50:55	Assessment of Dry Powder Inhaler Carrier Targeted Design	0:55	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.	0:150	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	4	42	theme	physical	736:743	arg1	properties					745:754	physical properties	736:754	physical properties	736:754	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	3	43	theme	new	466:468	arg1	carriers					478:485	new lactose carriers	466:485	new lactose carriers	466:485	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	0	44	theme	Dry	14:16	arg1	Powder					18:23	Dry Powder	14:23	Dry Powder	14:23	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	5	45	theme	feed	812:815	arg1	solutions					817:825	lactose-PEG 200 feed solutions	796:825	lactose-PEG 200 feed solutions of variable compositions	796:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	3	46	theme	process	408:414	arg1	aspects					416:422	different formulation and process aspects	382:422	different formulation and process aspects of spray-drying	382:438	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	4	47	theme	polyethylene	561:572	arg1	PEG					586:588	PEG 200	586:592	PEG 200	586:592	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	4	47	theme	polyethylene	561:572	arg1	glycol					574:579	polyethylene glycol 200	561:583	polyethylene glycol 200 (PEG 200)	561:593	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	7	48	theme	laser	1213:1217	arg1	diffraction					1219:1229	laser diffraction	1213:1229	laser diffraction	1213:1229	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	8	49	dep	blend	1521:1525	arg1	homogeneity					1527:1537	homogeneity	1527:1537	homogeneity	1527:1537	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	0	50	theme	Inhaler	25:31	arg1	Design					50:55	Assessment of Dry Powder Inhaler Carrier Targeted Design	0:55	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.	0:150	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	6	51	theme	wide-angle	1077:1086	arg1	scattering					1094:1103	wide-angle X-ray scattering	1077:1103	wide-angle X-ray scattering	1077:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	3	52	theme	formulation	392:402	arg1	aspects					416:422	different formulation and process aspects	382:422	different formulation and process aspects of spray-drying	382:438	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	10	53	theme	surface	1873:1879	arg1	properties					1881:1890	the shape, and surface properties	1858:1890	properties	1881:1890	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	0	54	theme	Targeted	41:48	arg1	Design					50:55	Assessment of Dry Powder Inhaler Carrier Targeted Design	0:55	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.	0:150	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	4	55	dep	in	604:605	arg1	vitro					607:611	vitro	607:611	vitro	607:611	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	8	56	theme	low	1487:1489	arg1	doses					1491:1495	low doses	1487:1495	low doses	1487:1495	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	7	57	theme	scanning	1330:1337	arg1	microscopy					1348:1357	scanning electron microscopy	1330:1357	scanning electron microscopy	1330:1357	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	5	58	theme	lactose	785:791	arg1	composition					770:780	The anomeric composition	757:780	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions	757:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	10	59	theme	particles	1954:1962	arg1	shape					1862:1866	the shape, and surface properties	1858:1890	shape	1862:1866	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	10	59	theme	particles	1954:1962	arg1	properties					1881:1890	the shape, and surface properties	1858:1890	properties	1881:1890	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	2	60	theme	carriers	358:365	arg1	engineering					339:349	engineering	339:349	engineering of new carriers	339:365	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	2	61	theme	inhaler	289:295	arg1	development					297:307	dry powder inhaler development	278:307	dry powder inhaler development	278:307	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	11	62	theme	blends	2079:2084	arg1	performance					2051:2061	the relatively inferior performance	2027:2061	the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites	2027:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	2	63	theme	dry	278:280	arg1	development					297:307	dry powder inhaler development	278:307	dry powder inhaler development	278:307	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	1	64	theme	pulmonary	156:164	arg1	landscape					181:189	The pulmonary administration landscape	152:189	The pulmonary administration landscape	152:189	The pulmonary administration landscape has rapidly advanced in recent years.
29856921	7	65	theme	properties	1196:1205	arg1	terms					1168:1172	terms	1168:1172	terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption	1168:1268	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	5	66	theme	anomeric	761:768	arg1	composition					770:780	The anomeric composition	757:780	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions	757:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	2	67	theme	invaluable	319:328	arg1	tool					330:333	an invaluable tool	316:333	an invaluable tool for engineering of new carriers	316:365	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	6	68	theme	resulting	991:999	arg1	particles					1013:1021	the resulting spray-dried particles	987:1021	the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering	987:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	10	69	theme	anomeric	1921:1928	arg1	composition					1930:1940	anomeric composition	1921:1940	anomeric composition	1921:1940	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	4	70	theme	particles	642:650	arg1	performance					613:623	the in vitro performance	600:623	the in vitro performance of the resulting particles	600:650	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	7	71	theme	selected	1119:1126	arg1	grades					1128:1133	The distinct selected grades	1106:1133	The distinct selected grades of lactose	1106:1144	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	9	72	theme	ratio	1703:1707	arg1	0.8:1					1709:1713	an anomeric ratio 0.8:1	1691:1713	an anomeric ratio 0.8:1 of α to β	1691:1723	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	11	73	theme	lactose-PEG	2123:2133	arg1	composites					2139:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	8	74	theme	lactose	1395:1401	arg1	carriers					1403:1410	the different lactose carriers	1381:1410	the different lactose carriers with inhalable-sized salbutamol sulfate	1381:1450	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	4	75	theme	in	604:605	arg1	performance					613:623	the in vitro performance	600:623	the in vitro performance of the resulting particles	600:650	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	5	76	theme	compositions	839:850	arg1	solutions					817:825	lactose-PEG 200 feed solutions	796:825	lactose-PEG 200 feed solutions of variable compositions	796:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	6	77	theme	anomeric	963:970	arg1	composition					972:982	the solid-state and anomeric composition	943:982	the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering	943:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	11	78	theme	spherical	2101:2109	arg1	composites					2139:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	the spherical spray-dried lactose-PEG 200 composites	2097:2148	This provided an insight into the relatively inferior performance of the adhesive blends containing the spherical spray-dried lactose-PEG 200 composites.
29856921	0	79	theme	Lactose	143:149	arg1	Properties					129:138	Physical Properties	120:138	Physical Properties of Lactose	120:149	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	0	79	theme	Lactose	143:149	arg1	Study					77:81	A Comparative Case Study	58:81	A Comparative Case Study of Diverse Anomeric Compositions	58:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	6	80	theme	solid-state	947:957	arg1	composition					972:982	the solid-state and anomeric composition	943:982	the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering	943:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	2	81	theme	particles	248:256	arg1	design					238:243	Targeted design	229:243	Targeted design of particles by spray-drying for dry powder inhaler development	229:307	Targeted design of particles by spray-drying for dry powder inhaler development offers an invaluable tool for engineering of new carriers.
29856921	5	82	theme	different	884:892	arg1	temperatures					894:905	different temperatures	884:905	different temperatures	884:905	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	6	83	theme	scanning	1052:1059	arg1	calorimetry					1061:1071	modulated differential scanning calorimetry	1029:1071	modulated differential scanning calorimetry	1029:1071	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	0	84	theme	Case	72:75	arg1	Study					77:81	A Comparative Case Study	58:81	A Comparative Case Study of Diverse Anomeric Compositions	58:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	6	85	theme	modulated	1029:1037	arg1	calorimetry					1061:1071	modulated differential scanning calorimetry	1029:1071	modulated differential scanning calorimetry	1029:1071	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	8	86	theme	Generation	1580:1589	arg1	Impactor					1591:1598	a Next Generation Impactor	1573:1598	a Next Generation Impactor	1573:1598	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	4	87	theme	other	670:674	arg1	grades					676:681	other grades	670:681	other grades of lactose with varying anomeric compositions and/or physical properties	670:754	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	7	88	theme	gas	1255:1257	arg1	adsorption					1259:1268	gas adsorption	1255:1268	gas adsorption	1255:1268	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	6	89	theme	particles	1013:1021	arg1	composition					972:982	the solid-state and anomeric composition	943:982	the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering	943:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	0	90	theme	Anomeric	94:101	arg1	Compositions					103:114	Diverse Anomeric Compositions	86:114	Diverse Anomeric Compositions	86:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	5	91	from	solutions	817:825	arg1	composition					770:780	The anomeric composition	757:780	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions	757:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	9	92	theme	particles	1637:1645	arg1	Characterization					1601:1616	Characterization	1601:1616	Characterization of the spray-dried particles	1601:1645	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	8	93	theme	salbutamol	1433:1442	arg1	sulfate					1444:1450	inhalable-sized salbutamol sulfate	1417:1450	inhalable-sized salbutamol sulfate	1417:1450	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	5	94	theme	lactose-PEG	796:806	arg1	solutions					817:825	lactose-PEG 200 feed solutions	796:825	lactose-PEG 200 feed solutions of variable compositions	796:850	The anomeric composition of lactose in lactose-PEG 200 feed solutions of variable compositions was analyzed via polarimetry at different temperatures.
29856921	3	95	theme	different	382:390	arg1	aspects					416:422	different formulation and process aspects	382:422	different formulation and process aspects of spray-drying	382:438	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	4	96	theme	anomeric	707:714	arg1	compositions					716:727	varying anomeric compositions	699:727	varying anomeric compositions	699:727	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	3	97	theme	spray-drying	427:438	arg1	aspects					416:422	different formulation and process aspects	382:422	different formulation and process aspects of spray-drying	382:438	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	0	98	theme	Powder	18:23	arg1	Assessment					0:9	Assessment	0:9	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.	0:150	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	9	99	theme	crystalline	1675:1685	arg1	particles					1736:1744	predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles	1661:1744	predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm	1661:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	4	100	theme	glycol	574:579	arg1	presence					549:556	the presence	545:556	the presence of polyethylene glycol 200 (PEG 200)	545:593	Using an integrated approach, lactose was spray-dried in the presence of polyethylene glycol 200 (PEG 200), and the in vitro performance of the resulting particles was compared with other grades of lactose with varying anomeric compositions and/or physical properties.
29856921	3	101	theme	lactose	470:476	arg1	carriers					478:485	new lactose carriers	466:485	new lactose carriers	466:485	In this work, different formulation and process aspects of spray-drying were exploited to produce new lactose carriers.
29856921	0	102	theme	Carrier	33:39	arg1	Design					50:55	Assessment of Dry Powder Inhaler Carrier Targeted Design	0:55	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.	0:150	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	0	103	dep	Design	50:55	arg1	Properties					129:138	Physical Properties	120:138	Physical Properties of Lactose	120:149	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	0	103	dep	Design	50:55	arg1	Study					77:81	A Comparative Case Study	58:81	A Comparative Case Study of Diverse Anomeric Compositions	58:114	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	6	104	theme	X-ray	1088:1092	arg1	scattering					1094:1103	wide-angle X-ray scattering	1077:1103	wide-angle X-ray scattering	1077:1103	These results were correlated with the solid-state and anomeric composition of the resulting spray-dried particles using modulated differential scanning calorimetry and wide-angle X-ray scattering.
29856921	7	105	theme	micromeritic	1183:1194	arg1	properties					1196:1205	their micromeritic properties	1177:1205	their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption	1177:1268	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	9	106	theme	anomeric	1694:1701	arg1	0.8:1					1709:1713	an anomeric ratio 0.8:1	1691:1713	an anomeric ratio 0.8:1 of α to β	1691:1723	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	7	107	theme	electron	1339:1346	arg1	microscopy					1348:1357	scanning electron microscopy	1330:1357	scanning electron microscopy	1330:1357	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29856921	0	108	theme	Physical	120:127	arg1	Properties					129:138	Physical Properties	120:138	Physical Properties of Lactose	120:149	Assessment of Dry Powder Inhaler Carrier Targeted Design: A Comparative Case Study of Diverse Anomeric Compositions and Physical Properties of Lactose.
29856921	9	109	dep	crystalline	1675:1685	arg1	0.8:1					1709:1713	an anomeric ratio 0.8:1	1691:1713	an anomeric ratio 0.8:1 of α to β	1691:1723	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	8	110	with	carriers	1403:1410	arg1	sulfate					1444:1450	inhalable-sized salbutamol sulfate	1417:1450	inhalable-sized salbutamol sulfate	1417:1450	Adhesive mixtures of the different lactose carriers with inhalable-sized salbutamol sulfate, as a model drug, were prepared in low doses and evaluated for their blend homogeneity and aerodynamic performance using a Next Generation Impactor.
29856921	9	111	with	particles	1736:1744	arg1	size					1758:1761	a mean size	1751:1761	a mean size of 50.9 ± 0.4 μm	1751:1778	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	9	112	theme	α	1718:1718	arg1	0.8:1					1709:1713	an anomeric ratio 0.8:1	1691:1713	an anomeric ratio 0.8:1 of α to β	1691:1723	Characterization of the spray-dried particles revealed that predominantly crystalline (in an anomeric ratio 0.8:1 of α to β) spherical particles with a mean size of 50.9 ± 0.4 μm could be produced.
29856921	10	113	theme	carrier	1946:1952	arg1	particles					1954:1962	carrier particles	1946:1962	carrier particles	1946:1962	Finally, it was apparent that micromeritic, in particular, the shape, and surface properties (inherent to solid-state and anomeric composition) of carrier particles dominantly control DPI delivery.
29856921	7	114	theme	helium	1232:1237	arg1	pycnometry					1239:1248	helium pycnometry	1232:1248	helium pycnometry	1232:1248	The distinct selected grades of lactose were characterized in terms of their micromeritic properties using laser diffraction, helium pycnometry, and gas adsorption, and their particle surface morphologies were evaluated via scanning electron microscopy.
29567106	8	0	theme	limited	2027:2033	arg1	range					2035:2039	a limited range	2025:2039	a limited range of application	2025:2054	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	8	1	theme	Most	1856:1859	arg1	approaches					1869:1878	Most current approaches	1856:1878	Most current approaches	1856:1878	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	6	2	theme	in	1402:1403	arg1	studies					1410:1416	In vitro and in vivo studies	1389:1416	In vitro and in vivo studies	1389:1416	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	4	3	theme	novel	991:995	arg1	approach					997:1004	this novel approach	986:1004	this novel approach	986:1004	We also demonstrate that this novel approach is applicable to a broad range of polysaccharides and acrylates.
29567106	9	4	with	efficiency	2336:2345	arg1	potential					2362:2370	excellent potential	2352:2370	excellent potential for industrial-scale production	2352:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	3	5	theme	acrylate	819:826	arg1	monomers					828:835	acrylate monomers	819:835	acrylate monomers	819:835	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	6	6	theme	In	1389:1390	arg1	studies					1410:1416	In vitro and in vivo studies	1389:1416	In vitro and in vivo studies	1389:1416	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	3	7	from	concentrations	588:601	arg1	formation					531:539	the kilogram-scale formation	512:539	the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt%	512:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	7	from	concentrations	588:601	arg1	nanocarriers					572:583	polysaccharide-polyacrylate nanocarriers	544:583	polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt%	544:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	2	8	theme	postpolymerization	322:339	arg1	strategy					355:362	a postpolymerization self-assembly strategy	320:362	a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application	320:479	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	6	9	dep	in	1402:1403	arg1	vivo					1405:1408	vivo	1405:1408	vivo	1405:1408	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	10	10	theme	unmatched	2574:2582	arg1	flexibility					2605:2615	the unmatched application-specific flexibility	2570:2615	the unmatched application-specific flexibility of nanocarriers	2570:2631	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	9	11	theme	industrial-scale	2376:2391	arg1	production					2393:2402	industrial-scale production	2376:2402	industrial-scale production	2376:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	8	12	theme	self-assembly	1908:1920	arg1	strategy					1922:1929	a post-polymerization self-assembly strategy	1886:1929	a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application	1886:2054	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	1	13	theme	industrial-scale	203:218	arg1	production					220:229	the industrial-scale production	199:229	the industrial-scale production of nanotherapeutics	199:249	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	4	14	theme	broad	1025:1029	arg1	range					1031:1035	a broad range	1023:1035	a broad range of polysaccharides and acrylates	1023:1068	We also demonstrate that this novel approach is applicable to a broad range of polysaccharides and acrylates.
29567106	9	15	theme	free	2113:2116	arg1	polymerization					2126:2139	free radical polymerization	2113:2139	free radical polymerization	2113:2139	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	3	16	theme	various	661:667	arg1	monomers					678:685	various acrylate monomers	661:685	various acrylate monomers	661:685	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	9	17	theme	excellent	2352:2360	arg1	potential					2362:2370	excellent potential	2352:2370	excellent potential for industrial-scale production	2352:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	8	18	theme	separate	1952:1959	arg1	step					1971:1974	a separate synthetic step	1950:1974	a separate synthetic step	1950:1974	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	10	19	theme	biological	2519:2528	arg1	functionalities					2530:2544	the biological functionalities	2515:2544	the biological functionalities of polysaccharides	2515:2563	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	10	20	theme	tumor	2479:2483	arg1	nanotheranostics					2485:2500	tumor nanotheranostics	2479:2500	tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers	2479:2631	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	5	21	theme	capping	1149:1155	arg1	groups					1157:1162	two vinyl capping groups	1139:1162	two vinyl capping groups	1139:1162	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	2	22	theme	current	295:301	arg1	approaches					303:312	Most current approaches	290:312	Most current approaches	290:312	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	9	23	theme	graft	2159:2163	arg1	GISA					2205:2208	GISA	2205:2208	GISA	2205:2208	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	9	23	theme	graft	2159:2163	arg1	self-assembly					2190:2202	the graft copolymerization-induced self-assembly	2155:2202	the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production	2155:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	7	24	dep	STATEMENT	1625:1633	arg1	recognized					1701:1710	recognized	1701:1710	have been recognized for their crucial roles in tumor theranostics	1691:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	3	25	theme	5 wt	612:615	arg1	%					616:616	5 wt%	612:616	5 wt%	612:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	7	26	theme	crucial	1722:1728	arg1	roles					1730:1734	their crucial roles	1716:1734	their crucial roles in tumor theranostics	1716:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	5	27	theme	nanotherapeutic	1364:1378	arg1	systems					1380:1386	anticancer nanotherapeutic systems	1353:1386	anticancer nanotherapeutic systems	1353:1386	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	3	28	theme	copolymerization	921:936	arg1	self-assembly					946:958	a graft copolymerization induced self-assembly	913:958	a graft copolymerization induced self-assembly	913:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	28	theme	copolymerization	921:936	arg1	process					881:887	a single process	872:887	a single process that is thus denoted as a graft copolymerization induced self-assembly	872:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	0	29	theme	drugs	84:88	arg1	delivery					59:66	the delivery	55:66	the delivery of chemotherapy drugs	55:88	Scalable and cleavable polysaccharide nanocarriers for the delivery of chemotherapy drugs.
29567106	1	30	theme	tumor	179:183	arg1	theranostics					185:196	tumor theranostics	179:196	tumor theranostics	179:196	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	8	31	theme	sub-gram	1999:2006	arg1	yields					2014:2019	sub-gram scale yields	1999:2019	sub-gram scale yields	1999:2019	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	6	32	theme	nanocarrier	1452:1462	arg1	use					1440:1442	the use	1436:1442	the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX)	1436:1514	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	7	33	theme	significant	1833:1843	arg1	challenge					1845:1853	a significant challenge	1831:1853	a significant challenge	1831:1853	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	3	34	from	backbones	857:865	arg1	copolymerization					799:814	the free radical graft copolymerization	776:814	the free radical graft copolymerization of acrylate monomers from polysaccharide backbones	776:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	9	35	theme	monomers	2223:2230	arg1	GISA					2205:2208	GISA	2205:2208	GISA	2205:2208	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	9	35	theme	monomers	2223:2230	arg1	self-assembly					2190:2202	the graft copolymerization-induced self-assembly	2155:2202	the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production	2155:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	1	36	theme	polysaccharide-based	97:116	arg1	nanocarriers					118:129	polysaccharide-based nanocarriers	97:129	polysaccharide-based nanocarriers	97:129	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	3	37	theme	acrylate	669:676	arg1	monomers					678:685	various acrylate monomers	661:685	various acrylate monomers	661:685	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	9	38	theme	various	2237:2243	arg1	hydrophobicities					2245:2260	various hydrophobicities	2237:2260	various hydrophobicities	2237:2260	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	2	39	theme	application	469:479	arg1	range					460:464	a limited range	450:464	a limited range of application	450:479	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	2	39	theme	application	469:479	arg1	yields					439:444	subgram scale yields	425:444	subgram scale yields	425:444	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	0	40	theme	cleavable	13:21	arg1	polysaccharide					23:36	cleavable polysaccharide	13:36	cleavable polysaccharide	13:36	Scalable and cleavable polysaccharide nanocarriers for the delivery of chemotherapy drugs.
29567106	7	41	theme	polysaccharide-based	1657:1676	arg1	nanocarriers					1678:1689	polysaccharide-based nanocarriers	1657:1689	polysaccharide-based nanocarriers	1657:1689	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	7	42	theme	nanotherapeutics	1800:1815	arg1	STATEMENT					1625:1633	STATEMENT	1625:1633	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics	1625:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	7	42	theme	nanotherapeutics	1800:1815	arg1	production					1780:1789	the industrial-scale production	1759:1789	the industrial-scale production of these nanotherapeutics	1759:1815	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	7	43	theme	SIGNIFICANCE	1638:1649	arg1	STATEMENT					1625:1633	STATEMENT	1625:1633	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics	1625:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	7	43	theme	SIGNIFICANCE	1638:1649	arg1	production					1780:1789	the industrial-scale production	1759:1789	the industrial-scale production of these nanotherapeutics	1759:1815	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	5	44	theme	disulfide	1119:1127	arg1	group					1129:1133	a disulfide group	1117:1133	a disulfide group	1117:1133	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	6	45	theme	hydrochloride	1496:1508	arg1	delivery					1472:1479	the delivery	1468:1479	the delivery of doxorubicin hydrochloride (DOX)	1468:1514	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	2	46	theme	synthetic	388:396	arg1	step					398:401	a separate synthetic step	377:401	a separate synthetic step	377:401	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	3	47	theme	hydrophobicity-induced	734:755	arg1	self-assembly					757:769	hydrophobicity-induced self-assembly	734:769	hydrophobicity-induced self-assembly	734:769	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	2	48	theme	subgram	425:431	arg1	yields					439:444	subgram scale yields	425:444	subgram scale yields	425:444	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	1	49	theme	significant	267:277	arg1	challenge					279:287	a significant challenge	265:287	a significant challenge	265:287	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	5	50	contain	bears	1111:1115	arg2	group					1129:1133	a disulfide group	1117:1133	a disulfide group	1117:1133	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	5	50	contain	bears	1111:1115	arg2	groups					1157:1162	two vinyl capping groups	1139:1162	two vinyl capping groups	1139:1162	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	5	50	contain	bears	1111:1115	arg1	crosslinker					1094:1104	a crosslinker	1092:1104	a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers	1092:1200	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	3	51	theme	graft	793:797	arg1	copolymerization					799:814	the free radical graft copolymerization	776:814	the free radical graft copolymerization of acrylate monomers from polysaccharide backbones	776:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	5	52	from	environments	1255:1266	arg1	cleavable					1229:1237	cleavable	1229:1237	cleavable	1229:1237	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	3	53	theme	nanocarriers	572:583	arg1	formation					531:539	the kilogram-scale formation	512:539	the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt%	512:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	9	54	theme	cleavable	2275:2283	arg1	nanocarriers					2313:2324	cleavable polysaccharide-polyacrylate nanocarriers	2275:2324	cleavable polysaccharide-polyacrylate nanocarriers	2275:2324	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	8	55	dep	follows	1942:1948	arg1	strategy					1922:1929	a post-polymerization self-assembly strategy	1886:1929	a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application	1886:2054	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	3	56	theme	free	780:783	arg1	radical					785:791	the free radical	776:791	the free radical graft copolymerization of acrylate monomers from polysaccharide backbones	776:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	8	57	theme	application	2044:2054	arg1	yields					2014:2019	sub-gram scale yields	1999:2019	sub-gram scale yields	1999:2019	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	8	57	theme	application	2044:2054	arg1	range					2035:2039	a limited range	2025:2039	a limited range of application	2025:2054	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	2	58	theme	self-assembly	341:353	arg1	strategy					355:362	a postpolymerization self-assembly strategy	320:362	a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application	320:479	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	6	59	theme	DOX	1560:1562	arg1	effects					1549:1555	the side effects	1540:1555	the side effects of DOX	1540:1562	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	5	60	theme	ideal	1312:1316	arg1	candidates					1318:1327	ideal candidates	1312:1327	ideal candidates for the construction of anticancer nanotherapeutic systems	1312:1386	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	6	61	dep	In	1389:1390	arg1	vitro					1392:1396	vitro	1392:1396	vitro	1392:1396	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	3	62	theme	monomers	828:835	arg1	copolymerization					799:814	the free radical graft copolymerization	776:814	the free radical graft copolymerization of acrylate monomers from polysaccharide backbones	776:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	63	theme	up	606:607	arg1	concentrations					588:601	concentrations	588:601	concentrations of up to 5 wt%	588:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	8	64	theme	current	1861:1867	arg1	approaches					1869:1878	Most current approaches	1856:1878	Most current approaches	1856:1878	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	9	65	theme	hydrophobic	2076:2086	arg1	effect					2088:2093	the hydrophobic effect	2072:2093	the hydrophobic effect	2072:2093	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	3	66	from	formation	531:539	arg1	concentrations					588:601	concentrations	588:601	concentrations of up to 5 wt%	588:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	67	theme	one-pot	628:634	arg1	approach					636:643	a one-pot approach	626:643	a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly	626:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	10	68	theme	polysaccharides	2549:2563	arg1	functionalities					2530:2544	the biological functionalities	2515:2544	the biological functionalities of polysaccharides	2515:2563	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	9	69	theme	polysaccharide-polyacrylate	2285:2311	arg1	nanocarriers					2313:2324	cleavable polysaccharide-polyacrylate nanocarriers	2275:2324	cleavable polysaccharide-polyacrylate nanocarriers	2275:2324	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	8	70	theme	post-polymerization	1888:1906	arg1	strategy					1922:1929	a post-polymerization self-assembly strategy	1886:1929	a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application	1886:2054	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	7	71	theme	industrial-scale	1763:1778	arg1	STATEMENT					1625:1633	STATEMENT	1625:1633	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics	1625:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	7	71	theme	industrial-scale	1763:1778	arg1	production					1780:1789	the industrial-scale production	1759:1789	the industrial-scale production of these nanotherapeutics	1759:1815	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	3	72	dep	approach	636:643	arg1	starting					647:654	starting	647:654	starting from various acrylate monomers and polysaccharides	647:705	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	73	theme	polysaccharide	842:855	arg1	backbones					857:865	polysaccharide backbones	842:865	polysaccharide backbones	842:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	1	74	theme	nanotherapeutics	234:249	arg1	production					220:229	the industrial-scale production	199:229	the industrial-scale production of nanotherapeutics	199:249	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	10	75	theme	application-specific	2584:2603	arg1	flexibility					2605:2615	the unmatched application-specific flexibility	2570:2615	the unmatched application-specific flexibility of nanocarriers	2570:2631	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	9	76	theme	radical	2118:2124	arg1	polymerization					2126:2139	free radical polymerization	2113:2139	free radical polymerization	2113:2139	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	7	77	theme	tumor	1739:1743	arg1	theranostics					1745:1756	tumor theranostics	1739:1756	tumor theranostics	1739:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	10	78	theme	nanocarriers	2620:2631	arg1	flexibility					2605:2615	the unmatched application-specific flexibility	2570:2615	the unmatched application-specific flexibility of nanocarriers	2570:2631	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	3	79	theme	single	874:879	arg1	self-assembly					946:958	a graft copolymerization induced self-assembly	913:958	a graft copolymerization induced self-assembly	913:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	79	theme	single	874:879	arg1	process					881:887	a single process	872:887	a single process that is thus denoted as a graft copolymerization induced self-assembly	872:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	5	80	theme	vinyl	1143:1147	arg1	groups					1157:1162	two vinyl capping groups	1139:1162	two vinyl capping groups	1139:1162	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	3	81	theme	kilogram-scale	516:529	arg1	formation					531:539	the kilogram-scale formation	512:539	the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt%	512:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	0	82	theme	chemotherapy	71:82	arg1	drugs					84:88	chemotherapy drugs	71:88	chemotherapy drugs	71:88	Scalable and cleavable polysaccharide nanocarriers for the delivery of chemotherapy drugs.
29567106	2	83	theme	Most	290:293	arg1	approaches					303:312	Most current approaches	290:312	Most current approaches	290:312	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	8	84	theme	synthetic	1961:1969	arg1	step					1971:1974	a separate synthetic step	1950:1974	a separate synthetic step	1950:1974	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	1	85	theme	crucial	162:168	arg1	roles					170:174	their crucial roles	156:174	their crucial roles in tumor theranostics	156:196	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	9	86	theme	copolymerization-induced	2165:2188	arg1	GISA					2205:2208	GISA	2205:2208	GISA	2205:2208	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	9	86	theme	copolymerization-induced	2165:2188	arg1	self-assembly					2190:2202	the graft copolymerization-induced self-assembly	2155:2202	the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production	2155:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	5	87	theme	anticancer	1353:1362	arg1	systems					1380:1386	anticancer nanotherapeutic systems	1353:1386	anticancer nanotherapeutic systems	1353:1386	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	6	88	theme	doxorubicin	1484:1494	arg1	DOX					1511:1513	DOX	1511:1513	DOX	1511:1513	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	6	88	theme	doxorubicin	1484:1494	arg1	hydrochloride					1496:1508	doxorubicin hydrochloride	1484:1508	doxorubicin hydrochloride (DOX)	1484:1514	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	5	89	theme	systems	1380:1386	arg1	construction					1337:1348	the construction	1333:1348	the construction of anticancer nanotherapeutic systems	1333:1386	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	3	90	theme	induced	938:944	arg1	self-assembly					946:958	a graft copolymerization induced self-assembly	913:958	a graft copolymerization induced self-assembly	913:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	90	theme	induced	938:944	arg1	process					881:887	a single process	872:887	a single process that is thus denoted as a graft copolymerization induced self-assembly	872:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	4	91	theme	polysaccharides	1040:1054	arg1	range					1031:1035	a broad range	1023:1035	a broad range of polysaccharides and acrylates	1023:1068	We also demonstrate that this novel approach is applicable to a broad range of polysaccharides and acrylates.
29567106	3	92	theme	graft	915:919	arg1	self-assembly					946:958	a graft copolymerization induced self-assembly	913:958	a graft copolymerization induced self-assembly	913:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	92	theme	graft	915:919	arg1	process					881:887	a single process	872:887	a single process that is thus denoted as a graft copolymerization induced self-assembly	872:958	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	1	93	from	roles	170:174	arg1	theranostics					185:196	tumor theranostics	179:196	tumor theranostics	179:196	While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of nanotherapeutics still remains a significant challenge.
29567106	4	94	theme	acrylates	1060:1068	arg1	range					1031:1035	a broad range	1023:1035	a broad range of polysaccharides and acrylates	1023:1068	We also demonstrate that this novel approach is applicable to a broad range of polysaccharides and acrylates.
29567106	9	95	theme	acrylate	2214:2221	arg1	monomers					2223:2230	acrylate monomers	2214:2230	acrylate monomers with various hydrophobicities	2214:2260	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	9	96	with	monomers	2223:2230	arg1	hydrophobicities					2245:2260	various hydrophobicities	2237:2260	various hydrophobicities	2237:2260	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	8	97	theme	scale	2008:2012	arg1	yields					2014:2019	sub-gram scale yields	1999:2019	sub-gram scale yields	1999:2019	Most current approaches adopt a post-polymerization self-assembly strategy which that follows a separate synthetic step, and thus suffers from sub-gram scale yields and a limited range of application.
29567106	10	98	theme	nanotheranostics	2485:2500	arg1	development					2464:2474	the development	2460:2474	the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers	2460:2631	We envision that these nanocarriers will contribute to the development of tumor nanotheranostics that combine the biological functionalities of polysaccharides with the unmatched application-specific flexibility of nanocarriers.
29567106	2	99	theme	scale	433:437	arg1	yields					439:444	subgram scale yields	425:444	subgram scale yields	425:444	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	6	100	theme	side	1544:1547	arg1	effects					1549:1555	the side effects	1540:1555	the side effects of DOX	1540:1562	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	5	101	theme	tumor	1283:1287	arg1	sites					1289:1293	tumor sites	1283:1293	tumor sites	1283:1293	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	5	102	from	cleavable	1229:1237	arg1	environments					1255:1266	the reducing environments	1242:1266	the reducing environments encountered at tumor sites	1242:1293	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	2	103	theme	limited	452:458	arg1	range					460:464	a limited range	450:464	a limited range of application	450:479	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	5	104	theme	reducing	1246:1253	arg1	environments					1255:1266	the reducing environments	1242:1266	the reducing environments encountered at tumor sites	1242:1293	Notably, by choosing a crosslinker that bears a disulfide group and two vinyl capping groups to structurally lock the nanocarriers, the products are rendered cleavable in the reducing environments encountered at tumor sites and thus provide ideal candidates for the construction of anticancer nanotherapeutic systems.
29567106	9	105	theme	high	2331:2334	arg1	efficiency					2336:2345	a high efficiency	2329:2345	a high efficiency with excellent potential for industrial-scale production	2329:2402	In this study, the hydrophobic effect was combined with free radical polymerization to facilitate the graft copolymerization-induced self-assembly (GISA) of acrylate monomers with various hydrophobicities to construct cleavable polysaccharide-polyacrylate nanocarriers at a high efficiency with excellent potential for industrial-scale production.
29567106	3	106	theme	self-assembly	757:769	arg1	aspects					723:729	aspects	723:729	aspects of hydrophobicity-induced self-assembly	723:769	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	3	107	theme	polysaccharide-polyacrylate	544:570	arg1	nanocarriers					572:583	polysaccharide-polyacrylate nanocarriers	544:583	polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt%	544:616	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
29567106	6	108	theme	chemotherapy	1599:1610	arg1	bio-safety					1581:1590	the bio-safety	1577:1590	the bio-safety of the chemotherapy accordingly	1577:1622	In vitro and in vivo studies demonstrated that the use of this nanocarrier for the delivery of doxorubicin hydrochloride (DOX) significantly decreased the side effects of DOX and improved the bio-safety of the chemotherapy accordingly.
29567106	7	109	from	roles	1730:1734	arg1	theranostics					1745:1756	tumor theranostics	1739:1756	tumor theranostics	1739:1756	STATEMENT OF SIGNIFICANCE While polysaccharide-based nanocarriers have been recognized for their crucial roles in tumor theranostics, the industrial-scale production of these nanotherapeutics still remains a significant challenge.
29567106	2	110	theme	separate	379:386	arg1	step					398:401	a separate synthetic step	377:401	a separate synthetic step	377:401	Most current approaches adopt a postpolymerization self-assembly strategy that follows a separate synthetic step and thus suffers from subgram scale yields and a limited range of application.
29567106	3	111	theme	radical	785:791	arg1	copolymerization					799:814	the free radical graft copolymerization	776:814	the free radical graft copolymerization of acrylate monomers from polysaccharide backbones	776:865	In this study, we demonstrate the kilogram-scale formation of polysaccharide-polyacrylate nanocarriers at concentrations of up to 5 wt% through a one-pot approach - starting from various acrylate monomers and polysaccharides - that combines aspects of hydrophobicity-induced self-assembly with the free radical graft copolymerization of acrylate monomers from polysaccharide backbones into a single process that is thus denoted as a graft copolymerization induced self-assembly.
30535915	10	0	theme	soil	2181:2184	arg1	structure					2186:2194	soil structure	2181:2194	soil structure	2181:2194	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	9	1	theme	inoculated	1932:1941	arg1	soils					1943:1947	the inoculated soils	1928:1947	the inoculated soils	1928:1947	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	7	2	from	particular	1637:1646	arg1	soils					1662:1666	the poorer soils	1651:1666	the poorer soils characterized by lower organic C contents	1651:1708	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	5	3	theme	higher	1216:1221	arg1	release					1223:1229	a higher release	1214:1229	a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS)	1214:1328	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	4	4	theme	species	906:912	arg1	inoculation					872:882	inoculation	872:882	inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing)	872:1011	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	9	5	theme	uronic	1817:1822	arg1	acids					1824:1828	uronic acids	1817:1828	uronic acids	1817:1828	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	9	5	theme	uronic	1817:1822	arg1	rhamnose					1882:1889	rhamnose	1882:1889	rhamnose	1882:1889	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	9	5	theme	uronic	1817:1822	arg1	fucose					1871:1876	fucose	1871:1876	fucose	1871:1876	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	7	6	from	soils	1662:1666	arg1	particular					1637:1646	particular	1637:1646	particular	1637:1646	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	10	7	theme	type	1993:1996	arg1	influence					1975:1983	the influence	1971:1983	the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils	1971:2241	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	7	8	theme	C	1699:1699	arg1	contents					1701:1708	lower organic C contents	1685:1708	lower organic C contents	1685:1708	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	9	9	theme	monosaccharides	1846:1860	arg1	abundance					1804:1812	Low abundance	1800:1812	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose,	1800:1890	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	0	10	theme	Induced	127:133	arg1	Biocrusts					135:143	Induced Biocrusts	127:143	Induced Biocrusts	127:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	5	11	theme	soluble	1101:1107	arg1	LB-EPS					1180:1185	LB-EPS	1180:1185	LB-EPS	1180:1185	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	11	theme	soluble	1101:1107	arg1	fraction					1132:1139	the more soluble and less condensed EPS fraction	1092:1139	the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS)	1092:1186	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	1	12	theme	biotechnological	215:230	arg1	Inoculation					146:156	Inoculation	146:156	Inoculation of soils with cyanobacteria	146:184	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	1	12	theme	biotechnological	215:230	arg1	technique					232:240	a sustainable biotechnological technique	201:240	a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	201:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	10	13	theme	restored	2220:2227	arg1	soils					2237:2241	restored dryland soils	2220:2241	restored dryland soils	2220:2241	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	0	14	from	Characteristics	78:92	arg1	Biocrusts					135:143	Induced Biocrusts	127:143	Induced Biocrusts	127:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	1	15	from	drylands	279:286	arg1	restoration					246:256	restoration	246:256	restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	246:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	10	16	theme	polysaccharidic	2071:2085	arg1	matrix					2087:2092	the polysaccharidic matrix	2067:2092	the polysaccharidic matrix	2067:2092	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	4	17	theme	study	746:750	arg1	objective					728:736	The objective	724:736	The objective of this study	724:750	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	1	18	from	areas	270:274	arg1	drylands					279:286	drylands	279:286	drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	279:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	7	19	theme	different	1547:1555	arg1	types					1557:1561	11 to 12 different types	1538:1561	11 to 12 different types of monosaccharides	1538:1580	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	2	20	theme	sandy	596:600	arg1	textures					607:614	sandy soil textures	596:614	sandy soil textures	596:614	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	0	21	from	Matrix	117:122	arg1	Biocrusts					135:143	Induced Biocrusts	127:143	Induced Biocrusts	127:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	5	22	theme	fraction	1274:1281	arg1	release					1223:1229	a higher release	1214:1229	a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS)	1214:1328	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	3	23	theme	cyanobacterial	653:666	arg1	matrix					684:689	the cyanobacterial polysaccharidic matrix	649:689	the cyanobacterial polysaccharidic matrix	649:689	However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type.
30535915	2	24	theme	cyanobacterial	516:529	arg1	biocrusts					531:539	induced cyanobacterial biocrusts	508:539	induced cyanobacterial biocrusts	508:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	4	25	theme	soil	1026:1029	arg1	types					1031:1035	different soil types	1016:1035	different soil types	1016:1035	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	1	26	theme	soils	161:165	arg1	Inoculation					146:156	Inoculation	146:156	Inoculation of soils with cyanobacteria	146:184	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	1	26	theme	soils	161:165	arg1	technique					232:240	a sustainable biotechnological technique	201:240	a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	201:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	2	27	theme	induced	508:514	arg1	biocrusts					531:539	induced cyanobacterial biocrusts	508:539	induced cyanobacterial biocrusts	508:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	4	28	from	environments	929:940	arg1	common					914:919	common	914:919	common	914:919	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	8	29	theme	high	1733:1736	arg1	abundances					1738:1747	high abundances	1733:1747	high abundances of galactose, mannose, and xylose	1733:1781	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	2	30	theme	existing	556:563	arg1	studies					565:571	the scarce existing studies	545:571	the scarce existing studies	545:571	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	4	31	theme	monosaccharidic	803:817	arg1	composition					819:829	monosaccharidic composition	803:829	monosaccharidic composition	803:829	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	5	32	theme	condensed	1260:1268	arg1	fraction					1274:1281	the less soluble and more condensed EPS fraction	1234:1281	the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS)	1234:1328	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	32	theme	condensed	1260:1268	arg1	TB-EPS					1322:1327	TB-EPS	1322:1327	TB-EPS	1322:1327	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	4	33	dep	Scytonema	982:990	arg1	javanicum					992:1000	Scytonema javanicum (N-fixing)	982:1011	Scytonema javanicum (N-fixing)	982:1011	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	5	34	theme	EPS	1128:1130	arg1	LB-EPS					1180:1185	LB-EPS	1180:1185	LB-EPS	1180:1185	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	34	theme	EPS	1128:1130	arg1	fraction					1132:1139	the more soluble and less condensed EPS fraction	1092:1139	the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS)	1092:1186	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	7	35	theme	complex	1496:1502	arg1	composition					1520:1530	a complex monosaccharidic composition	1494:1530	a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides)	1494:1581	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	2	36	theme	few	401:403	arg1	studies					405:411	few studies	401:411	few studies	401:411	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	0	37	theme	Matrix	117:122	arg1	Characteristics					78:92	the Macromolecular and Chemical Characteristics	46:92	the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts	46:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	1	38	with	Inoculation	146:156	arg1	cyanobacteria					172:184	cyanobacteria	172:184	cyanobacteria	172:184	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	4	39	from	distribution	786:797	arg1	types					1031:1035	different soil types	1016:1035	different soil types	1016:1035	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	7	40	theme	abundant	1609:1616	arg1	monosaccharide					1618:1631	the most abundant monosaccharide	1600:1631	the most abundant monosaccharide	1600:1631	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	5	41	dep	fraction	1170:1177	arg1	i.e.					1142:1145	i.e.	1142:1145	i.e.	1142:1145	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	0	42	theme	Chemical	69:76	arg1	Characteristics					78:92	the Macromolecular and Chemical Characteristics	46:92	the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts	46:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	10	43	from	influence	1975:1983	arg1	distribution					2020:2031	macromolecular distribution	2005:2031	macromolecular distribution	2005:2031	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	43	from	influence	1975:1983	arg1	composition					2052:2062	monosaccharide composition	2037:2062	monosaccharide composition	2037:2062	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	0	44	theme	Soil	0:3	arg1	Type					5:8	Soil Type	0:8	Soil Type	0:8	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	2	45	theme	matrix	498:503	arg1	characteristics					459:473	the macromolecular and chemical characteristics	427:473	the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts	427:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	5	46	theme	bound	1302:1306	arg1	fraction					1312:1319	the tightly bound EPS fraction	1290:1319	the tightly bound EPS fraction	1290:1319	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	47	theme	EPS	1166:1168	arg1	fraction					1170:1177	the loosely bound EPS fraction	1148:1177	the loosely bound EPS fraction	1148:1177	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	3	48	theme	matrix	684:689	arg1	characteristics					630:644	the characteristics	626:644	the characteristics of the cyanobacterial polysaccharidic matrix	626:689	However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type.
30535915	6	49	theme	MW	1367:1368	arg1	< 50 kDa					1381:1388	< 50 kDa	1381:1388	< 50 kDa	1381:1388	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	6	49	theme	MW	1367:1368	arg1	molecules					1370:1378	low MW molecules	1363:1378	low MW molecules (< 50 kDa)	1363:1389	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	10	50	theme	nutrient	2200:2207	arg1	cycling					2209:2215	nutrient cycling	2200:2215	nutrient cycling	2200:2215	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	5	51	dep	fraction	1132:1139	arg1	soil					1084:1087	soil	1084:1087	soil	1084:1087	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	51	dep	fraction	1132:1139	arg1	the					1080:1082	the	1080:1082	the	1080:1082	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	4	52	theme	polysaccharidic	838:852	arg1	matrix					854:859	the polysaccharidic matrix	834:859	the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing)	834:1011	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	10	53	from	composition	2052:2062	arg1	biocrusts					2105:2113	induced biocrusts	2097:2113	induced biocrusts	2097:2113	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	6	54	theme	high	1430:1433	arg1	1100-2000 kDa					1449:1461	1100-2000 kDa	1449:1461	1100-2000 kDa	1449:1461	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	6	54	theme	high	1430:1433	arg1	molecules					1438:1446	high MW molecules	1430:1446	high MW molecules (1100-2000 kDa)	1430:1462	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	1	55	theme	degraded	261:268	arg1	areas					270:274	degraded areas	261:274	degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	261:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	10	56	theme	soil	1988:1991	arg1	type					1993:1996	soil type	1988:1996	soil type	1988:1996	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	8	57	theme	xylose	1776:1781	arg1	abundances					1738:1747	high abundances	1733:1747	high abundances of galactose, mannose, and xylose	1733:1781	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	4	58	theme	arid	924:927	arg1	environments					929:940	arid environments	924:940	arid environments	924:940	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	9	59	theme	Low	1800:1802	arg1	abundance					1804:1812	Low abundance	1800:1812	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose,	1800:1890	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	1	60	theme	due	288:290	arg1	drylands					279:286	drylands	279:286	drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	279:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	10	61	theme	macromolecular	2005:2018	arg1	distribution					2020:2031	macromolecular distribution	2005:2031	macromolecular distribution	2005:2031	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	4	62	theme	cyanobacterial	891:904	arg1	ambiguum					954:961	Phormidium ambiguum	943:961	Phormidium ambiguum (non N-fixing)	943:976	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	62	theme	cyanobacterial	891:904	arg1	Scytonema					982:990	Scytonema	982:990	Scytonema	982:990	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	62	theme	cyanobacterial	891:904	arg1	species					906:912	two cyanobacterial species	887:912	two cyanobacterial species common in arid environments	887:940	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	9	63	theme	acids	1824:1828	arg1	abundance					1804:1812	Low abundance	1800:1812	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose,	1800:1890	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	4	64	theme	common	914:919	arg1	ambiguum					954:961	Phormidium ambiguum	943:961	Phormidium ambiguum (non N-fixing)	943:976	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	64	theme	common	914:919	arg1	Scytonema					982:990	Scytonema	982:990	Scytonema	982:990	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	64	theme	common	914:919	arg1	species					906:912	two cyanobacterial species	887:912	two cyanobacterial species common in arid environments	887:940	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	7	65	theme	organic	1691:1697	arg1	contents					1701:1708	lower organic C contents	1685:1708	lower organic C contents	1685:1708	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	9	66	theme	hydrophobic	1834:1844	arg1	monosaccharides					1846:1860	hydrophobic monosaccharides	1834:1860	hydrophobic monosaccharides	1834:1860	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	9	66	theme	hydrophobic	1834:1844	arg1	rhamnose					1882:1889	rhamnose	1882:1889	rhamnose	1882:1889	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	9	66	theme	hydrophobic	1834:1844	arg1	fucose					1871:1876	fucose	1871:1876	fucose	1871:1876	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	1	67	theme	sustainable	203:213	arg1	Inoculation					146:156	Inoculation	146:156	Inoculation of soils with cyanobacteria	146:184	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	1	67	theme	sustainable	203:213	arg1	technique					232:240	a sustainable biotechnological technique	201:240	a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	201:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	7	68	theme	monosaccharides	1566:1580	arg1	types					1557:1561	11 to 12 different types	1538:1561	11 to 12 different types of monosaccharides	1538:1580	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	10	69	theme	dryland	2229:2235	arg1	soils					2237:2241	restored dryland soils	2220:2241	restored dryland soils	2220:2241	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	70	theme	matrix	2087:2092	arg1	distribution					2020:2031	macromolecular distribution	2005:2031	macromolecular distribution	2005:2031	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	70	theme	matrix	2087:2092	arg1	composition					2052:2062	monosaccharide composition	2037:2062	monosaccharide composition	2037:2062	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	5	71	theme	condensed	1118:1126	arg1	LB-EPS					1180:1185	LB-EPS	1180:1185	LB-EPS	1180:1185	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	71	theme	condensed	1118:1126	arg1	fraction					1132:1139	the more soluble and less condensed EPS fraction	1092:1139	the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS)	1092:1186	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	2	72	theme	soil	602:605	arg1	textures					607:614	sandy soil textures	596:614	sandy soil textures	596:614	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	7	73	dep	12	1544:1545	arg1	to					1541:1542	to	1541:1542	to	1541:1542	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	5	74	theme	EPS	1270:1272	arg1	fraction					1274:1281	the less soluble and more condensed EPS fraction	1234:1281	the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS)	1234:1328	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	74	theme	EPS	1270:1272	arg1	TB-EPS					1322:1327	TB-EPS	1322:1327	TB-EPS	1322:1327	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	1	75	from	restoration	246:256	arg1	drylands					279:286	drylands	279:286	drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	279:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	7	76	theme	lower	1685:1689	arg1	contents					1701:1708	lower organic C contents	1685:1708	lower organic C contents	1685:1708	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	4	77	theme	non	964:966	arg1	ambiguum					954:961	Phormidium ambiguum	943:961	Phormidium ambiguum (non N-fixing)	943:976	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	77	theme	non	964:966	arg1	N-fixing					968:975	non N-fixing	964:975	non N-fixing	964:975	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	10	78	theme	monosaccharide	2037:2050	arg1	composition					2052:2062	monosaccharide composition	2037:2062	monosaccharide composition	2037:2062	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	6	79	theme	low	1363:1365	arg1	< 50 kDa					1381:1388	< 50 kDa	1381:1388	< 50 kDa	1381:1388	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	6	79	theme	low	1363:1365	arg1	molecules					1370:1378	low MW molecules	1363:1378	low MW molecules (< 50 kDa)	1363:1389	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	4	80	theme	different	1016:1024	arg1	types					1031:1035	different soil types	1016:1035	different soil types	1016:1035	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	1	81	dep	role	309:312	arg1	play					368:371	play	368:371	play in the environment	368:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	10	82	theme	induced	2097:2103	arg1	biocrusts					2105:2113	induced biocrusts	2097:2113	induced biocrusts	2097:2113	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	5	83	theme	soluble	1243:1249	arg1	fraction					1274:1281	the less soluble and more condensed EPS fraction	1234:1281	the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS)	1234:1328	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	5	83	theme	soluble	1243:1249	arg1	TB-EPS					1322:1327	TB-EPS	1322:1327	TB-EPS	1322:1327	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	8	84	theme	C-rich	1719:1724	arg1	soils					1726:1730	more C-rich soils	1714:1730	more C-rich soils	1714:1730	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	7	85	theme	poorer	1655:1660	arg1	soils					1662:1666	the poorer soils	1651:1666	the poorer soils characterized by lower organic C contents	1651:1708	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	7	86	theme	monosaccharidic	1504:1518	arg1	composition					1520:1530	a complex monosaccharidic composition	1494:1530	a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides)	1494:1581	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	2	87	from	characteristics	459:473	arg1	biocrusts					531:539	induced cyanobacterial biocrusts	508:539	induced cyanobacterial biocrusts	508:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	10	88	from	development	2151:2161	arg1	soils					2237:2241	restored dryland soils	2220:2241	restored dryland soils	2220:2241	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	88	from	development	2151:2161	arg1	structure					2186:2194	soil structure	2181:2194	soil structure	2181:2194	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	88	from	development	2151:2161	arg1	cycling					2209:2215	nutrient cycling	2200:2215	nutrient cycling	2200:2215	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	0	89	theme	Polysaccharidic	101:115	arg1	Matrix					117:122	the Polysaccharidic Matrix	97:122	the Polysaccharidic Matrix in Induced Biocrusts	97:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	5	90	theme	bound	1160:1164	arg1	fraction					1170:1177	the loosely bound EPS fraction	1148:1177	the loosely bound EPS fraction	1148:1177	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	7	91	from	types	1557:1561	arg1	composition					1520:1530	a complex monosaccharidic composition	1494:1530	a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides)	1494:1581	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	4	92	from	composition	819:829	arg1	types					1031:1035	different soil types	1016:1035	different soil types	1016:1035	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	2	93	theme	scarce	549:554	arg1	studies					565:571	the scarce existing studies	545:571	the scarce existing studies	545:571	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	5	94	theme	EPS	1308:1310	arg1	fraction					1312:1319	the tightly bound EPS fraction	1290:1319	the tightly bound EPS fraction	1290:1319	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	4	95	from	common	914:919	arg1	environments					929:940	arid environments	924:940	arid environments	924:940	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	8	96	theme	galactose	1752:1760	arg1	abundances					1738:1747	high abundances	1733:1747	high abundances of galactose, mannose, and xylose	1733:1781	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	2	97	theme	chemical	450:457	arg1	characteristics					459:473	the macromolecular and chemical characteristics	427:473	the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts	427:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	10	98	from	role	2173:2176	arg1	soils					2237:2241	restored dryland soils	2220:2241	restored dryland soils	2220:2241	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	98	from	role	2173:2176	arg1	structure					2186:2194	soil structure	2181:2194	soil structure	2181:2194	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	98	from	role	2173:2176	arg1	cycling					2209:2215	nutrient cycling	2200:2215	nutrient cycling	2200:2215	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	10	99	theme	biocrust	2142:2149	arg1	development					2151:2161	biocrust development	2142:2161	biocrust development	2142:2161	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	1	100	theme	important	299:307	arg1	role					309:312	the important role	295:312	the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	295:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30535915	8	101	theme	mannose	1763:1769	arg1	abundances					1738:1747	high abundances	1733:1747	high abundances of galactose, mannose, and xylose	1733:1781	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	8	102	located	found	1793:1797	arg2	abundances					1738:1747	high abundances	1733:1747	high abundances of galactose, mannose, and xylose	1733:1781	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	8	102	located	found	1793:1797	arg1	soils					1726:1730	more C-rich soils	1714:1730	more C-rich soils	1714:1730	In more C-rich soils, high abundances of galactose, mannose, and xylose were also found.
30535915	2	103	theme	macromolecular	431:444	arg1	characteristics					459:473	the macromolecular and chemical characteristics	427:473	the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts	427:539	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	4	104	theme	macromolecular	771:784	arg1	distribution					786:797	the macromolecular distribution	767:797	the macromolecular distribution	767:797	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	5	105	theme	higher	1062:1067	arg1	release					1069:1075	a higher release	1060:1075	a higher release	1060:1075	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	2	106	theme	polysaccharidic	482:496	arg1	matrix					498:503	the polysaccharidic matrix	478:503	the polysaccharidic matrix	478:503	So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures.
30535915	3	107	theme	polysaccharidic	668:682	arg1	matrix					684:689	the cyanobacterial polysaccharidic matrix	649:689	the cyanobacterial polysaccharidic matrix	649:689	However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type.
30535915	0	108	theme	Macromolecular	50:63	arg1	Characteristics					78:92	the Macromolecular and Chemical Characteristics	46:92	the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts	46:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	7	109	theme	EPS	1473:1475	arg1	fractions					1477:1485	The two EPS fractions	1465:1485	The two EPS fractions	1465:1485	The two EPS fractions showed a complex monosaccharidic composition (from 11 to 12 different types of monosaccharides), with glucose as the most abundant monosaccharide, in particular in the poorer soils characterized by lower organic C contents.
30535915	10	110	dep	distribution	2020:2031	arg1	the					2001:2003	the	2001:2003	the	2001:2003	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	3	111	theme	soil	713:716	arg1	type					718:721	soil type	713:721	soil type	713:721	However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type.
30535915	9	112	located	found	1896:1900	arg2	abundance					1804:1812	Low abundance	1800:1812	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose,	1800:1890	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	9	112	located	found	1896:1900	arg1	EPS					1909:1911	the EPS	1905:1911	the EPS extracted from the inoculated soils	1905:1947	Low abundance of uronic acids and hydrophobic monosaccharides, such as fucose and rhamnose, was found in the EPS extracted from the inoculated soils.
30535915	5	113	dep	fraction	1312:1319	arg1	i.e.					1284:1287	i.e.	1284:1287	i.e.	1284:1287	S. javanicum promoted a higher release in the soil of the more soluble and less condensed EPS fraction (i.e., the loosely bound EPS fraction, LB-EPS), while P. ambiguum showed a higher release of the less soluble and more condensed EPS fraction (i.e., the tightly bound EPS fraction, TB-EPS).
30535915	10	114	from	distribution	2020:2031	arg1	biocrusts					2105:2113	induced biocrusts	2097:2113	induced biocrusts	2097:2113	Our results point to the influence of soil type on the macromolecular distribution and monosaccharide composition of the polysaccharidic matrix in induced biocrusts, which is likely to affect biocrust development and their role in soil structure and nutrient cycling in restored dryland soils.
30535915	6	115	theme	MW	1435:1436	arg1	1100-2000 kDa					1449:1461	1100-2000 kDa	1449:1461	1100-2000 kDa	1449:1461	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	6	115	theme	MW	1435:1436	arg1	molecules					1438:1446	high MW molecules	1430:1446	high MW molecules (1100-2000 kDa)	1430:1462	LB-EPSs were mainly composed of low MW molecules (< 50 kDa), while TB-EPSs were mainly composed of high MW molecules (1100-2000 kDa).
30535915	0	116	dep	Type	5:8	arg1	Species					28:34	Species	28:34	Species	28:34	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	0	117	from	Biocrusts	135:143	arg1	Characteristics					78:92	the Macromolecular and Chemical Characteristics	46:92	the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts	46:143	Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.
30535915	4	118	theme	matrix	854:859	arg1	distribution					786:797	the macromolecular distribution	767:797	the macromolecular distribution	767:797	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	4	118	theme	matrix	854:859	arg1	composition					819:829	monosaccharidic composition	803:829	monosaccharidic composition	803:829	The objective of this study was to examine the macromolecular distribution and monosaccharidic composition of the polysaccharidic matrix induced by inoculation of two cyanobacterial species common in arid environments, Phormidium ambiguum (non N-fixing) and Scytonema javanicum (N-fixing) in different soil types.
30535915	1	119	theme	areas	270:274	arg1	restoration					246:256	restoration	246:256	restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment	246:390	Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment.
30529355	7	0	theme	new	1029:1031	arg1	structures					1037:1046	the new gel structures	1025:1046	the new gel structures	1025:1046	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	3	1	theme	gel	482:484	arg1	structure					491:499	gel type structure	482:499	gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	482:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	10	2	theme	mechanism	1310:1318	arg1	dependence					1292:1301	the dependence	1288:1301	the dependence of the mechanism of release on the composition of the gels	1288:1360	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	6	3	theme	gels	960:963	arg1	composition					932:942	The chemical composition	919:942	The chemical composition of bioconjugate gels	919:963	The chemical composition of bioconjugate gels was confirmed by FTIR spectroscopy.
30529355	3	4	theme	type	486:489	arg1	structure					491:499	gel type structure	482:499	gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	482:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	10	5	theme	in	1265:1266	arg1	release					1274:1280	The in vitro release	1261:1280	The in vitro release	1261:1280	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	0	6	theme	spiroacetal	94:104	arg1	moieties					106:113	pendant spiroacetal moieties	86:113	pendant spiroacetal moieties	86:113	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	6	7	theme	bioconjugate	947:958	arg1	gels					960:963	bioconjugate gels	947:963	bioconjugate gels	947:963	The chemical composition of bioconjugate gels was confirmed by FTIR spectroscopy.
30529355	9	8	theme	drug	1238:1241	arg1	structures					1212:1221	The new structures	1204:1221	The new structures	1204:1221	The new structures were tested as drug delivery systems.
30529355	9	8	theme	drug	1238:1241	arg1	systems					1252:1258	drug delivery systems	1238:1258	drug delivery systems	1238:1258	The new structures were tested as drug delivery systems.
30529355	0	9	theme	pendant	86:92	arg1	moieties					106:113	pendant spiroacetal moieties	86:113	pendant spiroacetal moieties	86:113	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	5	10	from	effects	855:861	arg1	properties					893:902	various properties	885:902	various properties	885:902	The effects of the composition on various properties were assessed.
30529355	9	11	theme	delivery	1243:1250	arg1	structures					1212:1221	The new structures	1204:1221	The new structures	1204:1221	The new structures were tested as drug delivery systems.
30529355	9	11	theme	delivery	1243:1250	arg1	systems					1252:1258	drug delivery systems	1238:1258	drug delivery systems	1238:1258	The new structures were tested as drug delivery systems.
30529355	10	12	from	dependence	1292:1301	arg1	composition					1338:1348	the composition	1334:1348	the composition of the gels	1334:1360	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	11	13	theme	good	1481:1484	arg1	biocompatibility					1486:1501	a good biocompatibility	1479:1501	a good biocompatibility	1479:1501	In vivo investigations demonstrated a good biocompatibility after systemic administration in mice.
30529355	6	14	theme	chemical	923:930	arg1	composition					932:942	The chemical composition	919:942	The chemical composition of bioconjugate gels	919:963	The chemical composition of bioconjugate gels was confirmed by FTIR spectroscopy.
30529355	1	15	dep	elasticity	120:129	arg1	soft					132:135	soft	132:135	soft	132:135	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	16	theme	human	171:175	arg1	body					177:180	the human body	167:180	the human body	167:180	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	0	17	theme	hybrid	16:21	arg1	network					26:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	4	18	theme	poly	738:741	arg1	copolymer					815:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	10	19	theme	release	1323:1329	arg1	mechanism					1310:1318	the mechanism	1306:1318	the mechanism of release	1306:1329	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	0	20	theme	Multifunctional	0:14	arg1	network					26:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	10	21	dep	in	1265:1266	arg1	vitro					1268:1272	vitro	1268:1272	vitro	1268:1272	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	3	22	theme	relevant	541:548	arg1	biomaterial					550:560	a relevant biomaterial	539:560	a relevant biomaterial for its antimicrobial properties	539:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	3	22	theme	relevant	541:548	arg1	quercetin					515:523	quercetin	515:523	quercetin	515:523	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	11	23	theme	systemic	1509:1516	arg1	administration					1518:1531	systemic administration	1509:1531	systemic administration in mice	1509:1539	In vivo investigations demonstrated a good biocompatibility after systemic administration in mice.
30529355	4	24	theme	natural/synthetic	654:670	arg1	structure					672:680	the natural/synthetic structure	650:680	the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin	650:848	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	9	25	theme	new	1208:1210	arg1	structures					1212:1221	The new structures	1204:1221	The new structures	1204:1221	The new structures were tested as drug delivery systems.
30529355	9	25	theme	new	1208:1210	arg1	systems					1252:1258	drug delivery systems	1238:1258	drug delivery systems	1238:1258	The new structures were tested as drug delivery systems.
30529355	4	26	theme	itaconic	743:750	arg1	copolymer					815:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	10	27	theme	gels	1357:1360	arg1	composition					1338:1348	the composition	1334:1348	the composition of the gels	1334:1360	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	1	28	theme	important	186:194	arg1	characteristics					196:210	important characteristics	186:210	important characteristics of the gels	186:222	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	28	theme	important	186:194	arg1	similarity					153:162	similarity	153:162	similarity to the human body	153:180	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	28	theme	important	186:194	arg1	consistency					137:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	0	29	theme	3D	23:24	arg1	network					26:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network	0:32	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	7	30	theme	enhanced	1053:1060	arg1	capacity					1071:1078	enhanced swelling capacity	1053:1078	enhanced swelling capacity	1053:1078	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	4	31	theme	gel	693:695	arg1	based					697:701	complex gel based	685:701	complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	685:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	5	32	theme	composition	870:880	arg1	effects					855:861	The effects	851:861	The effects of the composition on various properties	851:902	The effects of the composition on various properties were assessed.
30529355	3	33	theme	new	384:386	arg1	strategy					388:395	a new strategy	382:395	a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	382:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	8	34	dep	gels	1146:1149	arg1	responsiveness					1151:1164	responsiveness	1151:1164	responsiveness to stimuli	1151:1175	The investigations evidenced the gels responsiveness to stimuli and their high elasticity.
30529355	3	35	theme	antimicrobial	570:582	arg1	properties					584:593	its antimicrobial properties	566:593	its antimicrobial properties	566:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	7	36	theme	swelling	1062:1069	arg1	capacity					1071:1078	enhanced swelling capacity	1053:1078	enhanced swelling capacity	1053:1078	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	4	37	theme	preparation	620:630	arg1	possibilities					632:644	the preparation possibilities	616:644	the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin	616:848	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	11	38	theme	In	1443:1444	arg1	investigations					1451:1464	In vivo investigations	1443:1464	In vivo investigations	1443:1464	In vivo investigations demonstrated a good biocompatibility after systemic administration in mice.
30529355	7	39	theme	typical	1086:1092	arg1	behavior					1103:1110	a typical gel-like behavior	1084:1110	a typical gel-like behavior	1084:1110	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	10	40	theme	super	1428:1432	arg1	case					1434:1437	super case II	1428:1440	super case II	1428:1440	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	10	41	theme	transport	1376:1384	arg1	behavior					1386:1393	a transport behavior	1374:1393	a transport behavior which varies from the Fickian to super case II	1374:1440	The in vitro release shows the dependence of the mechanism of release on the composition of the gels, evidencing a transport behavior which varies from the Fickian to super case II.
30529355	4	42	theme	hyaluronic	706:715	arg1	acid					717:720	hyaluronic acid	706:720	hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	706:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	0	43	theme	hyaluronic	43:52	arg1	acid					54:57	hyaluronic acid	43:57	hyaluronic acid	43:57	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	6	44	theme	FTIR	982:985	arg1	spectroscopy					987:998	FTIR spectroscopy	982:998	FTIR spectroscopy	982:998	The chemical composition of bioconjugate gels was confirmed by FTIR spectroscopy.
30529355	7	45	theme	gel-like	1094:1101	arg1	behavior					1103:1110	a typical gel-like behavior	1084:1110	a typical gel-like behavior	1084:1110	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	11	46	from	administration	1518:1531	arg1	mice					1536:1539	mice	1536:1539	mice	1536:1539	In vivo investigations demonstrated a good biocompatibility after systemic administration in mice.
30529355	1	47	theme	elasticity	120:129	arg1	characteristics					196:210	important characteristics	186:210	important characteristics of the gels	186:222	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	47	theme	elasticity	120:129	arg1	similarity					153:162	similarity	153:162	similarity to the human body	153:180	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	47	theme	elasticity	120:129	arg1	consistency					137:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	11	48	dep	In	1443:1444	arg1	vivo					1446:1449	vivo	1446:1449	vivo	1446:1449	In vivo investigations demonstrated a good biocompatibility after systemic administration in mice.
30529355	2	49	theme	Such	225:228	arg1	similarities					230:241	Such similarities	225:241	Such similarities	225:241	Such similarities allow the hydrogels to be biocompatible and, as a consequence, they are very attractive to be applied as biomaterials.
30529355	3	50	theme	natural/synthetic	443:459	arg1	conjugate					469:477	a natural/synthetic polymer conjugate	441:477	a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	441:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	1	51	theme	gels	219:222	arg1	characteristics					196:210	important characteristics	186:210	important characteristics of the gels	186:222	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	51	theme	gels	219:222	arg1	similarity					153:162	similarity	153:162	similarity to the human body	153:180	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	1	51	theme	gels	219:222	arg1	consistency					137:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency	116:147	The elasticity, soft consistency and similarity to the human body are important characteristics of the gels.
30529355	4	52	theme	undecane	805:812	arg1	copolymer					815:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	738:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	7	53	theme	gel	1033:1035	arg1	structures					1037:1046	the new gel structures	1025:1046	the new gel structures	1025:1046	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	3	54	theme	polymer	461:467	arg1	conjugate					469:477	a natural/synthetic polymer conjugate	441:477	a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	441:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	5	55	theme	various	885:891	arg1	properties					893:902	various properties	885:902	various properties	885:902	The effects of the composition on various properties were assessed.
30529355	0	56	contain	containing	75:84	arg1	copolymer					65:73	a copolymer	63:73	a copolymer containing pendant spiroacetal moieties	63:113	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	0	56	contain	containing	75:84	arg2	moieties					106:113	pendant spiroacetal moieties	86:113	pendant spiroacetal moieties	86:113	Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.
30529355	4	57	theme	complex	685:691	arg1	based					697:701	complex gel based	685:701	complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	685:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	3	58	theme	biohybrid	414:422	arg1	complex					424:430	a biohybrid complex	412:430	a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties	412:593	This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties.
30529355	8	59	theme	high	1187:1190	arg1	elasticity					1192:1201	their high elasticity	1181:1201	their high elasticity	1181:1201	The investigations evidenced the gels responsiveness to stimuli and their high elasticity.
30529355	4	60	from	based	697:701	arg1	acid					717:720	hyaluronic acid	706:720	hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer	706:823	There are evidenced the preparation possibilities for the natural/synthetic structure as complex gel based on hyaluronic acid conjugated with poly(itaconic anhydride‑co‑3,9‑divinyl‑2,4,8,10‑tetraoxaspiro[5.5] undecane) copolymer decorated with quercetin.
30529355	7	61	contain	have	1048:1051	arg2	capacity					1071:1078	enhanced swelling capacity	1053:1078	enhanced swelling capacity	1053:1078	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	7	61	contain	have	1048:1051	arg1	structures					1037:1046	the new gel structures	1025:1046	the new gel structures	1025:1046	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30529355	7	61	contain	have	1048:1051	arg2	behavior					1103:1110	a typical gel-like behavior	1084:1110	a typical gel-like behavior	1084:1110	It was established that the new gel structures have enhanced swelling capacity and a typical gel-like behavior.
30041037	2	0	theme	crude	349:353	arg1	F					364:364	F	364:364	F	364:364	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	2	0	theme	crude	349:353	arg1	extract					355:361	the crude extract	345:361	the crude extract (F)	345:365	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	6	1	theme	hepatocarcinoma	933:947	arg1	cells					955:959	murine hepatocarcinoma Hca-F cells	926:959	murine hepatocarcinoma Hca-F cells	926:959	F, F1 and F2 showed limited cytotoxicity against murine hepatocarcinoma Hca-F cells in vitro.
30041037	5	2	theme	pure	856:859	arg1	components					811:820	two components	807:820	two components	807:820	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	2	theme	pure	856:859	arg1	fucan					870:874	a pure sulfated fucan	854:874	a pure sulfated fucan	854:874	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	0	3	from	characterization	11:26	arg1	algae					74:78	brown algae	68:78	brown algae Kjellmaniella crassifolia farmed in northern China	68:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	7	4	theme	Hca-F-inoculated	1060:1075	arg1	mice					1077:1080	Hca-F-inoculated mice	1060:1080	Hca-F-inoculated mice	1060:1080	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	8	5	theme	crude	1241:1245	arg1	extract					1247:1253	the crude extract	1237:1253	the crude extract	1237:1253	F1 and F2 did not show competitive antineoplastic efficacy, as did the crude extract.
30041037	3	6	dep	1D	642:643	arg1	spectroscopy					685:696	nuclear magnetic resonance (NMR) spectroscopy	652:696	nuclear magnetic resonance (NMR) spectroscopy	652:696	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	7	theme	magnetic	660:667	arg1	resonance					669:677	nuclear magnetic resonance	652:677	nuclear magnetic resonance (NMR) spectroscopy	652:696	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	7	theme	magnetic	660:667	arg1	NMR					680:682	NMR	680:682	NMR	680:682	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	7	8	theme	lump	1045:1048	arg1	growth					1050:1055	lump growth	1045:1055	lump growth	1045:1055	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	3	9	theme	liquid	561:566	arg1	HPLC					584:587	HPLC	584:587	HPLC	584:587	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	9	theme	liquid	561:566	arg1	chromatography					568:581	high-performance liquid chromatography	544:581	high-performance liquid chromatography (HPLC)	544:588	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	10	theme	isolated	503:510	arg1	fractions					512:520	the isolated fractions	499:520	the isolated fractions	499:520	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	0	11	theme	farmed	106:111	arg1	algae					74:78	brown algae	68:78	brown algae Kjellmaniella crassifolia farmed in northern China	68:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	3	12	theme	nuclear	652:658	arg1	resonance					669:677	nuclear magnetic resonance	652:677	nuclear magnetic resonance (NMR) spectroscopy	652:696	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	12	theme	nuclear	652:658	arg1	NMR					680:682	NMR	680:682	NMR	680:682	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	13	theme	fractions	512:520	arg1	composition					453:463	monosaccharide composition	438:463	monosaccharide composition	438:463	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	13	theme	fractions	512:520	arg1	characteristics					480:494	structural characteristics	469:494	structural characteristics	469:494	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	7	14	from	dose	1001:1004	arg1	administration					976:989	Oral administration	971:989	Oral administration of F at a dose of 450 mg/kg d	971:1019	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	7	15	theme	tumor	1123:1127	arg1	tissues					1129:1135	tumor tissues	1123:1135	tumor tissues	1123:1135	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	2	16	dep	fractions	407:415	arg1	F2					422:423	F2	422:423	F2	422:423	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	2	16	dep	fractions	407:415	arg1	F1					418:419	F1	418:419	F1	418:419	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	2	16	dep	fractions	407:415	arg1	fractions					407:415	three fractions	401:415	three fractions (F1, F2 and F3)	401:431	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	2	16	dep	fractions	407:415	arg1	F3					429:430	F3	429:430	F3	429:430	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	5	17	contain	has	803:805	arg2	fucan					870:874	a pure sulfated fucan	854:874	a pure sulfated fucan	854:874	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	17	contain	has	803:805	arg1	F3					800:801	F3	800:801	F3	800:801	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	17	contain	has	803:805	arg2	components					811:820	two components	807:820	two components	807:820	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	17	contain	has	803:805	arg2	galactofucan					837:848	an acetylated galactofucan	823:848	an acetylated galactofucan	823:848	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	0	18	theme	northern	116:123	arg1	China					125:129	northern China	116:129	northern China	116:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	7	19	from	expression	1109:1118	arg1	tissues					1129:1135	tumor tissues	1123:1135	tumor tissues	1123:1135	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	4	20	theme	glucuronic	783:792	arg1	acid					794:797	glucuronic acid	783:797	glucuronic acid	783:797	F1 is an acetylated galactofucan, and F2 consists of fucose, galactose, mannose and glucuronic acid.
30041037	0	21	dep	farmed	106:111	arg1	crassifolia					94:104	crassifolia	94:104	crassifolia	94:104	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	0	22	from	effects	42:48	arg1	algae					74:78	brown algae	68:78	brown algae Kjellmaniella crassifolia farmed in northern China	68:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	10	23	theme	efficacy	1330:1337	arg1	molecules					1356:1364	the small molecules	1346:1364	the small molecules	1346:1364	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	10	23	theme	efficacy	1330:1337	arg1	origin					1316:1321	The origin	1312:1321	The origin of its efficacy	1312:1337	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	10	23	theme	efficacy	1330:1337	arg1	phenols					1375:1381	phenols	1375:1381	phenols that attached to native fucoidan	1375:1414	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	3	24	dep	composition	453:463	arg1	The					434:436	The	434:436	The	434:436	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	2	25	dep	crassifolia	271:281	arg1	farmed					284:289	farmed	284:289	farmed	284:289	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	0	26	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	6	27	theme	limited	897:903	arg1	cytotoxicity					905:916	limited cytotoxicity	897:916	limited cytotoxicity	897:916	F, F1 and F2 showed limited cytotoxicity against murine hepatocarcinoma Hca-F cells in vitro.
30041037	7	28	theme	450 mg/kg d	1009:1019	arg1	dose					1001:1004	a dose	999:1004	a dose of 450 mg/kg d	999:1019	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	3	29	theme	high-performance	544:559	arg1	HPLC					584:587	HPLC	584:587	HPLC	584:587	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	29	theme	high-performance	544:559	arg1	chromatography					568:581	high-performance liquid chromatography	544:581	high-performance liquid chromatography (HPLC)	544:588	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	10	30	theme	small	1350:1354	arg1	molecules					1356:1364	the small molecules	1346:1364	the small molecules	1346:1364	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	10	30	theme	small	1350:1354	arg1	origin					1316:1321	The origin	1312:1321	The origin of its efficacy	1312:1337	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	10	30	theme	small	1350:1354	arg1	phenols					1375:1381	phenols	1375:1381	phenols that attached to native fucoidan	1375:1414	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	0	31	theme	antitumor	32:40	arg1	effects					42:48	antitumor effects	32:48	antitumor effects	32:48	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	9	32	theme	Crude	1256:1260	arg1	fucoidan					1262:1269	Crude fucoidan	1256:1269	Crude fucoidan	1256:1269	Crude fucoidan could be a promising antitumor adjuvant.
30041037	9	32	theme	Crude	1256:1260	arg1	adjuvant					1302:1309	adjuvant	1302:1309	adjuvant	1302:1309	Crude fucoidan could be a promising antitumor adjuvant.
30041037	10	33	theme	native	1400:1405	arg1	fucoidan					1407:1414	native fucoidan	1400:1414	native fucoidan	1400:1414	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	4	34	theme	acetylated	708:717	arg1	F1					699:700	F1	699:700	F1	699:700	F1 is an acetylated galactofucan, and F2 consists of fucose, galactose, mannose and glucuronic acid.
30041037	4	34	theme	acetylated	708:717	arg1	galactofucan					719:730	an acetylated galactofucan	705:730	an acetylated galactofucan	705:730	F1 is an acetylated galactofucan, and F2 consists of fucose, galactose, mannose and glucuronic acid.
30041037	1	35	theme	bioactivities	197:209	arg1	variety					186:192	a variety	184:192	a variety of bioactivities	184:209	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	1	35	theme	bioactivities	197:209	arg1	bioactivities					197:209	bioactivities	197:209	bioactivities	197:209	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	7	36	theme	FAS	1105:1107	arg1	expression					1109:1118	upregulated FAS expression	1093:1118	upregulated FAS expression in tumor tissues	1093:1135	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	10	37	attach	attached	1388:1395	arg2	phenols					1375:1381	phenols	1375:1381	phenols that attached to native fucoidan	1375:1414	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	10	37	attach	attached	1388:1395	arg1	fucoidan					1407:1414	native fucoidan	1400:1414	native fucoidan	1400:1414	The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan.
30041037	3	38	theme	monosaccharide	438:451	arg1	composition					453:463	monosaccharide composition	438:463	monosaccharide composition	438:463	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	7	39	theme	F	994:994	arg1	administration					976:989	Oral administration	971:989	Oral administration of F at a dose of 450 mg/kg d	971:1019	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	8	40	theme	antineoplastic	1205:1218	arg1	efficacy					1220:1227	competitive antineoplastic efficacy	1193:1227	competitive antineoplastic efficacy	1193:1227	F1 and F2 did not show competitive antineoplastic efficacy, as did the crude extract.
30041037	8	41	theme	competitive	1193:1203	arg1	efficacy					1220:1227	competitive antineoplastic efficacy	1193:1227	competitive antineoplastic efficacy	1193:1227	F1 and F2 did not show competitive antineoplastic efficacy, as did the crude extract.
30041037	3	42	theme	infrared	609:616	arg1	FTIR					632:635	FTIR	632:635	FTIR	632:635	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	42	theme	infrared	609:616	arg1	spectroscopy					618:629	Fourier-transform infrared spectroscopy	591:629	Fourier-transform infrared spectroscopy (FTIR)	591:636	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	0	43	from	algae	74:78	arg1	fucoidans					53:61	fucoidans	53:61	fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China	53:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	0	43	from	algae	74:78	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	0	43	from	algae	74:78	arg1	effects					42:48	antitumor effects	32:48	antitumor effects	32:48	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	7	44	theme	upregulated	1093:1103	arg1	expression					1109:1118	upregulated FAS expression	1093:1118	upregulated FAS expression in tumor tissues	1093:1135	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	1	45	link	alga-derived	138:149	arg1	fucoidan					151:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	1	46	contain	have	179:182	arg1	fucoidan					151:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	1	46	contain	have	179:182	arg2	bioactivities					197:209	bioactivities	197:209	bioactivities	197:209	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	1	46	contain	have	179:182	arg2	variety					186:192	a variety	184:192	a variety of bioactivities	184:209	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	2	47	theme	antitumor	227:235	arg1	effect					237:242	the antitumor effect	223:242	the antitumor effect of fucoidan	223:254	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	0	48	theme	fucoidans	53:61	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	0	48	theme	fucoidans	53:61	arg1	effects					42:48	antitumor effects	32:48	antitumor effects	32:48	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	0	49	from	China	125:129	arg1	farmed					106:111	farmed	106:111	farmed	106:111	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	8	50	dep	did	1233:1235	arg1	as					1230:1231	as	1230:1231	as	1230:1231	F1 and F2 did not show competitive antineoplastic efficacy, as did the crude extract.
30041037	1	51	theme	Brown	132:136	arg1	fucoidan					151:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	2	52	from	farmed	284:289	arg1	China					302:306	China	302:306	China	302:306	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	3	53	theme	Fourier-transform	591:607	arg1	FTIR					632:635	FTIR	632:635	FTIR	632:635	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	3	53	theme	Fourier-transform	591:607	arg1	spectroscopy					618:629	Fourier-transform infrared spectroscopy	591:629	Fourier-transform infrared spectroscopy (FTIR)	591:636	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	1	54	theme	alga-derived	138:149	arg1	fucoidan					151:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan	132:158	Brown alga-derived fucoidan has been proven to have a variety of bioactivities.
30041037	7	55	theme	Oral	971:974	arg1	administration					976:989	Oral administration	971:989	Oral administration of F at a dose of 450 mg/kg d	971:1019	Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control.
30041037	2	56	theme	Kjellmaniella	257:269	arg1	crassifolia					271:281	Kjellmaniella crassifolia	257:281	Kjellmaniella crassifolia (farmed in Dalian, China)	257:307	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	5	57	theme	sulfated	861:868	arg1	components					811:820	two components	807:820	two components	807:820	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	57	theme	sulfated	861:868	arg1	fucan					870:874	a pure sulfated fucan	854:874	a pure sulfated fucan	854:874	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	0	58	from	farmed	106:111	arg1	China					125:129	northern China	116:129	northern China	116:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	3	59	theme	structural	469:478	arg1	characteristics					480:494	structural characteristics	469:494	structural characteristics	469:494	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	6	60	theme	murine	926:931	arg1	cells					955:959	murine hepatocarcinoma Hca-F cells	926:959	murine hepatocarcinoma Hca-F cells	926:959	F, F1 and F2 showed limited cytotoxicity against murine hepatocarcinoma Hca-F cells in vitro.
30041037	0	61	theme	brown	68:72	arg1	algae					74:78	brown algae	68:78	brown algae Kjellmaniella crassifolia farmed in northern China	68:129	Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.
30041037	3	62	theme	resonance	669:677	arg1	spectroscopy					685:696	nuclear magnetic resonance (NMR) spectroscopy	652:696	nuclear magnetic resonance (NMR) spectroscopy	652:696	The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy.
30041037	2	63	theme	fucoidan	247:254	arg1	effect					237:242	the antitumor effect	223:242	the antitumor effect of fucoidan	223:254	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	5	64	theme	acetylated	826:835	arg1	galactofucan					837:848	an acetylated galactofucan	823:848	an acetylated galactofucan	823:848	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	5	64	theme	acetylated	826:835	arg1	components					811:820	two components	807:820	two components	807:820	F3 has two components, an acetylated galactofucan and a pure sulfated fucan.
30041037	2	65	from	China	302:306	arg1	farmed					284:289	farmed	284:289	farmed	284:289	To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3).
30041037	6	66	theme	Hca-F	949:953	arg1	cells					955:959	murine hepatocarcinoma Hca-F cells	926:959	murine hepatocarcinoma Hca-F cells	926:959	F, F1 and F2 showed limited cytotoxicity against murine hepatocarcinoma Hca-F cells in vitro.
29329813	0	0	theme	antioxidant	63:73	arg1	activity					75:82	their in vitro antioxidant activity	48:82	their in vitro antioxidant activity	48:82	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	2	1	from	composition	400:410	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	2	2	from	distribution	434:445	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	1	3	theme	neutral	227:233	arg1	TPSI					256:259	TPSI	256:259	TPSI	256:259	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	3	theme	neutral	227:233	arg1	polysaccharides					239:253	neutral tea polysaccharides	227:253	neutral tea polysaccharides (TPSI)	227:260	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	0	4	from	Effects	0:6	arg1	polysaccharides					29:43	tea polysaccharides	25:43	tea polysaccharides	25:43	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	0	4	from	Effects	0:6	arg1	activity					75:82	their in vitro antioxidant activity	48:82	their in vitro antioxidant activity	48:82	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	0	4	from	Effects	0:6	arg1	activity					101:108	hypoglycemic activity	88:108	hypoglycemic activity	88:108	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	1	5	theme	tea	235:237	arg1	TPSI					256:259	TPSI	256:259	TPSI	256:259	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	5	theme	tea	235:237	arg1	polysaccharides					239:253	neutral tea polysaccharides	227:253	neutral tea polysaccharides (TPSI)	227:260	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	6	theme	antioxidant	474:484	arg1	activities					486:495	the 4 in vitro antioxidant activities	459:495	the 4 in vitro antioxidant activities	459:495	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	1	7	theme	exchange	310:317	arg1	D315					325:328	anion exchange resin D315	304:328	anion exchange resin D315	304:328	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	6	8	theme	products	1151:1158	arg1	development					1091:1101	the development	1087:1101	the development of polysaccharide antioxidants and hypoglycemic products	1087:1158	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	6	9	theme	potential	1064:1072	arg1	pruning					1014:1020	pruning leaves	1014:1027	pruning leaves of tea plant	1014:1040	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	6	9	theme	potential	1064:1072	arg1	resource					1074:1081	a potential resource	1062:1081	a potential resource for the development of polysaccharide antioxidants and hypoglycemic products	1062:1158	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	3	10	theme	metal	766:770	arg1	ions					772:775	their metal ions	760:775	their metal ions	760:775	By comparing TTPS and TPSII, we found that they exhibited different antioxidant activities and inhibitory actions against α-glucosidase after their metal ions were removed.
29329813	1	11	theme	resin	319:323	arg1	D315					325:328	anion exchange resin D315	304:328	anion exchange resin D315	304:328	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	4	12	dep	in	804:805	arg1	vitro					807:811	vitro	807:811	vitro	807:811	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	6	13	theme	hypoglycemic	1138:1149	arg1	products					1151:1158	hypoglycemic products	1138:1158	hypoglycemic products	1138:1158	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	6	14	theme	tea	1032:1034	arg1	plant					1036:1040	tea plant	1032:1040	tea plant	1032:1040	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	0	15	theme	hypoglycemic	88:99	arg1	activity					101:108	hypoglycemic activity	88:108	hypoglycemic activity	88:108	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	3	16	theme	different	676:684	arg1	activities					698:707	different antioxidant activities	676:707	different antioxidant activities	676:707	By comparing TTPS and TPSII, we found that they exhibited different antioxidant activities and inhibitory actions against α-glucosidase after their metal ions were removed.
29329813	4	17	theme	antioxidant	813:823	arg1	activities					825:834	in vitro antioxidant activities	804:834	in vitro antioxidant activities	804:834	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	6	18	dep	pruning	1014:1020	arg1	leaves					1022:1027	leaves	1022:1027	leaves	1022:1027	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	6	19	theme	plant	1036:1040	arg1	pruning					1014:1020	pruning leaves	1014:1027	pruning leaves of tea plant	1014:1040	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	6	19	theme	plant	1036:1040	arg1	resource					1074:1081	a potential resource	1062:1081	a potential resource for the development of polysaccharide antioxidants and hypoglycemic products	1062:1158	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	2	20	theme	molecular	417:425	arg1	distribution					434:445	molecular weight distribution	417:445	molecular weight distribution	417:445	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	3	21	theme	antioxidant	686:696	arg1	activities					698:707	different antioxidant activities	676:707	different antioxidant activities	676:707	By comparing TTPS and TPSII, we found that they exhibited different antioxidant activities and inhibitory actions against α-glucosidase after their metal ions were removed.
29329813	4	22	theme	inhibitory	840:849	arg1	effects					851:857	inhibitory effects	840:857	inhibitory effects on α-glucosidase	840:874	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	6	23	theme	antioxidants	1121:1132	arg1	development					1091:1101	the development	1087:1101	the development of polysaccharide antioxidants and hypoglycemic products	1087:1158	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	0	24	from	polysaccharides	29:43	arg1	Effects					0:6	Effects	0:6	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity	0:108	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	2	25	theme	physicochemical	336:350	arg1	properties					352:361	Some physicochemical properties	331:361	Some physicochemical properties	331:361	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	2	26	theme	metal	588:592	arg1	ions					594:597	metal ions	588:597	metal ions	588:597	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	6	27	theme	polysaccharide	1106:1119	arg1	antioxidants					1121:1132	polysaccharide antioxidants	1106:1132	polysaccharide antioxidants	1106:1132	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	0	28	theme	ions	17:20	arg1	Effects					0:6	Effects	0:6	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity	0:108	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	0	29	dep	in	54:55	arg1	vitro					57:61	vitro	57:61	vitro	57:61	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	2	30	theme	monosaccharide	385:398	arg1	composition					400:410	monosaccharide composition	385:410	monosaccharide composition	385:410	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	0	31	theme	metal	11:15	arg1	ions					17:20	metal ions	11:20	metal ions in tea polysaccharides	11:43	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	3	32	theme	inhibitory	713:722	arg1	actions					724:730	inhibitory actions	713:730	inhibitory actions	713:730	By comparing TTPS and TPSII, we found that they exhibited different antioxidant activities and inhibitory actions against α-glucosidase after their metal ions were removed.
29329813	2	33	dep	in	465:466	arg1	vitro					468:472	vitro	468:472	vitro	468:472	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	6	34	theme	same	985:988	arg1	time					990:993	the same time	981:993	the same time	981:993	At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
29329813	1	35	theme	pruning	177:183	arg1	kinds					168:172	two kinds	164:172	two kinds of pruning leaves of tea plant	164:203	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	0	36	theme	tea	25:27	arg1	polysaccharides					29:43	tea polysaccharides	25:43	tea polysaccharides	25:43	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	2	37	theme	inhibitory	501:510	arg1	effects					512:518	inhibitory effects	501:518	inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions	501:597	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	1	38	theme	acidic	266:271	arg1	TPSII					294:298	TPSII	294:298	TPSII	294:298	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	38	theme	acidic	266:271	arg1	polysaccharides					277:291	acidic tea polysaccharides	266:291	acidic tea polysaccharides (TPSII)	266:299	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	4	39	dep	activities	825:834	arg1	the					800:802	the	800:802	the	800:802	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	2	40	theme	in	465:466	arg1	activities					486:495	the 4 in vitro antioxidant activities	459:495	the 4 in vitro antioxidant activities	459:495	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	1	41	theme	tea	273:275	arg1	TPSII					294:298	TPSII	294:298	TPSII	294:298	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	41	theme	tea	273:275	arg1	polysaccharides					277:291	acidic tea polysaccharides	266:291	acidic tea polysaccharides (TPSII)	266:299	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	42	theme	polysaccharides	546:560	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	4	43	from	activities	825:834	arg1	α-glucosidase					862:874	α-glucosidase	862:874	α-glucosidase	862:874	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	1	44	theme	anion	304:308	arg1	exchange					310:317	anion exchange	304:317	anion exchange resin D315	304:328	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	45	theme	above	540:544	arg1	polysaccharides					546:560	above polysaccharides	540:560	above polysaccharides	540:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	2	46	theme	weight	427:432	arg1	distribution					434:445	molecular weight distribution	417:445	molecular weight distribution	417:445	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	4	47	theme	in	804:805	arg1	activities					825:834	in vitro antioxidant activities	804:834	in vitro antioxidant activities	804:834	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	1	48	theme	Total	111:115	arg1	TTPS					138:141	TTPS	138:141	TTPS	138:141	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	48	theme	Total	111:115	arg1	polysaccharides					121:135	Total tea polysaccharides	111:135	Total tea polysaccharides (TTPS)	111:142	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	49	from	properties	352:361	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	1	50	theme	tea	117:119	arg1	TTPS					138:141	TTPS	138:141	TTPS	138:141	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	1	50	theme	tea	117:119	arg1	polysaccharides					121:135	Total tea polysaccharides	111:135	Total tea polysaccharides (TTPS)	111:142	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	5	51	theme	certain	932:938	arg1	reference					940:948	a certain reference	930:948	a certain reference for tea and soil selection	930:975	The study can be a certain reference for tea and soil selection.
29329813	5	51	theme	certain	932:938	arg1	study					917:921	The study	913:921	The study	913:921	The study can be a certain reference for tea and soil selection.
29329813	1	52	theme	tea	195:197	arg1	plant					199:203	tea plant	195:203	tea plant	195:203	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	53	from	activities	486:495	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	2	54	from	structure	374:382	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
29329813	5	55	dep	tea	954:956	arg1	selection					967:975	selection	967:975	selection	967:975	The study can be a certain reference for tea and soil selection.
29329813	1	56	theme	plant	199:203	arg1	pruning					177:183	pruning leaves	177:190	pruning leaves of tea plant	177:203	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	0	57	theme	in	54:55	arg1	activity					75:82	their in vitro antioxidant activity	48:82	their in vitro antioxidant activity	48:82	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	4	58	from	effects	851:857	arg1	α-glucosidase					862:874	α-glucosidase	862:874	α-glucosidase	862:874	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	4	59	theme	TPSI	879:882	arg1	activities					825:834	in vitro antioxidant activities	804:834	in vitro antioxidant activities	804:834	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	4	59	theme	TPSI	879:882	arg1	effects					851:857	inhibitory effects	840:857	inhibitory effects on α-glucosidase	840:874	However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved.
29329813	0	60	from	ions	17:20	arg1	polysaccharides					29:43	tea polysaccharides	25:43	tea polysaccharides	25:43	Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity.
29329813	1	61	dep	pruning	177:183	arg1	leaves					185:190	leaves	185:190	leaves	185:190	Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315.
29329813	2	62	from	effects	512:518	arg1	α-glucosidase					523:535	α-glucosidase	523:535	α-glucosidase of above polysaccharides	523:560	Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated.
30093019	6	0	theme	highest	991:997	arg1	activity					1011:1018	the highest antioxidant activity	987:1018	the highest antioxidant activity	987:1018	Glu-CPS-1 exhibited the highest antioxidant activity suggested culture conditions could regulate the bioactivity through influencing the structure and properties.
30093019	5	1	from	activity	888:895	arg1	ABTS+					900:904	ABTS+	900:904	ABTS+	900:904	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	5	1	from	activity	888:895	arg1	radicals					919:926	hydroxyl radicals	910:926	hydroxyl radicals	910:926	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	5	1	from	activity	888:895	arg1	radical					958:964	DPPH radical	953:964	DPPH radical	953:964	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	7	2	from	application	1172:1182	arg1	development					1234:1244	the development	1230:1244	the development of polysaccharides with high antioxidant activity	1230:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	7	3	theme	polysaccharides	1249:1263	arg1	development					1234:1244	the development	1230:1244	the development of polysaccharides with high antioxidant activity	1230:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	7	4	with	polysaccharides	1249:1263	arg1	activity					1287:1294	high antioxidant activity	1270:1294	high antioxidant activity	1270:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	0	5	from	Effect	0:5	arg1	activities					79:88	antioxidant activities	67:88	antioxidant activities	67:88	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	5	from	Effect	0:5	arg1	properties					52:61	physicochemical properties	36:61	physicochemical properties	36:61	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	1	6	dep	polysaccharides	142:156	arg1	polysaccharides					142:156	Three polysaccharides	136:156	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1)	136:193	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	1	6	dep	polysaccharides	142:156	arg1	Glu-CPS-1					184:192	Glu-CPS-1	184:192	Glu-CPS-1	184:192	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	1	6	dep	polysaccharides	142:156	arg1	NaCl-CPS-1					169:178	NaCl-CPS-1	169:178	NaCl-CPS-1	169:178	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	1	6	dep	polysaccharides	142:156	arg1	WL-CPS-1					159:166	WL-CPS-1	159:166	WL-CPS-1	159:166	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	0	7	theme	polysaccharides	93:107	arg1	activities					79:88	antioxidant activities	67:88	antioxidant activities	67:88	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	7	theme	polysaccharides	93:107	arg1	properties					52:61	physicochemical properties	36:61	physicochemical properties	36:61	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	8	from	properties	52:61	arg1	Nostoc					114:119	Nostoc	114:119	Nostoc	114:119	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	3	9	with	acid	577:580	arg1	ratios					597:602	different ratios	587:602	different ratios	587:602	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	1	10	theme	mixotrophic	279:289	arg1	conditions					299:308	normal, salt stress and mixotrophic culture conditions	255:308	conditions	299:308	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	4	11	theme	surface	773:779	arg1	morphology					781:790	different surface morphology	763:790	different surface morphology	763:790	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	7	12	theme	conditions	1216:1225	arg1	regulation					1194:1203	proper regulation	1187:1203	proper regulation of culture conditions	1187:1225	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	5	13	theme	strong	870:875	arg1	activity					888:895	strong scavenging activity	870:895	strong scavenging activity on ABTS+ and hydroxyl radicals	870:926	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	4	14	theme	similar	715:721	arg1	fourier					723:729	similar fourier transform infrared spectra, but different surface morphology, chain length and branching	715:818	fourier	723:729	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	7	15	theme	high	1270:1273	arg1	activity					1287:1294	high antioxidant activity	1270:1294	high antioxidant activity	1270:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	7	16	theme	antioxidant	1275:1285	arg1	activity					1287:1294	high antioxidant activity	1270:1294	high antioxidant activity	1270:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	3	17	theme	different	587:595	arg1	ratios					597:602	different ratios	587:602	different ratios	587:602	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	5	18	theme	moderate	932:939	arg1	activity					941:948	moderate activity	932:948	moderate activity on DPPH radical	932:964	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	3	19	theme	chemical	438:445	arg1	composition					447:457	Glu-CPS-1 chemical composition	428:457	Glu-CPS-1 chemical composition	428:457	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	3	20	theme	average	614:620	arg1	weights					632:638	the average molecular weights	610:638	the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa	610:683	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	3	21	theme	uronic	481:486	arg1	acid					488:491	uronic acid	481:491	uronic acid	481:491	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	5	22	theme	scavenging	877:886	arg1	activity					888:895	strong scavenging activity	870:895	strong scavenging activity on ABTS+ and hydroxyl radicals	870:926	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	1	23	theme	culture	291:297	arg1	conditions					299:308	normal, salt stress and mixotrophic culture conditions	255:308	conditions	299:308	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	3	24	with	monosaccharides	546:560	arg1	ratios					597:602	different ratios	587:602	different ratios	587:602	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	3	25	theme	molecular	622:630	arg1	weights					632:638	the average molecular weights	610:638	the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa	610:683	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	3	26	theme	acid	488:491	arg1	contents					493:500	sugar and uronic acid contents	471:500	sugar and uronic acid contents	471:500	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	0	27	theme	conditions	18:27	arg1	Effect					0:5	Effect	0:5	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc	0:119	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	4	28	theme	chain	793:797	arg1	length					799:804	chain length	793:804	chain length	793:804	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	7	29	theme	proper	1187:1192	arg1	regulation					1194:1203	proper regulation	1187:1203	proper regulation of culture conditions	1187:1225	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	2	30	theme	physicochemical	330:344	arg1	properties					346:355	Their physicochemical properties	324:355	Their physicochemical properties	324:355	Their physicochemical properties and antioxidant activities were investigated.
30093019	3	31	theme	Glu-CPS-1	428:436	arg1	composition					447:457	Glu-CPS-1 chemical composition	428:457	Glu-CPS-1 chemical composition	428:457	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	7	32	theme	regulation	1194:1203	arg1	application					1172:1182	the potential application	1158:1182	the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity	1158:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	6	33	dep	suggested	1020:1028	arg1	regulate					1055:1062	regulate	1055:1062	suggested culture conditions could regulate the bioactivity through influencing the structure and properties	1020:1127	Glu-CPS-1 exhibited the highest antioxidant activity suggested culture conditions could regulate the bioactivity through influencing the structure and properties.
30093019	5	34	theme	DPPH	953:956	arg1	radical					958:964	DPPH radical	953:964	DPPH radical	953:964	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	3	35	theme	sugar	471:475	arg1	contents					493:500	sugar and uronic acid contents	471:500	sugar and uronic acid contents	471:500	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	0	36	from	activities	79:88	arg1	Nostoc					114:119	Nostoc	114:119	Nostoc	114:119	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	37	dep	properties	52:61	arg1	the					32:34	the	32:34	the	32:34	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	5	38	theme	hydroxyl	910:917	arg1	radicals					919:926	hydroxyl radicals	910:926	hydroxyl radicals	910:926	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	1	39	theme	Nostoc	228:233	arg1	flagelliforme					235:247	Nostoc flagelliforme	228:247	Nostoc flagelliforme	228:247	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	6	40	theme	culture	1030:1036	arg1	conditions					1038:1047	culture conditions	1030:1047	culture conditions	1030:1047	Glu-CPS-1 exhibited the highest antioxidant activity suggested culture conditions could regulate the bioactivity through influencing the structure and properties.
30093019	3	41	theme	constituent	534:544	arg1	monosaccharides					546:560	nine constituent monosaccharides	529:560	nine constituent monosaccharides	529:560	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	0	42	theme	physicochemical	36:50	arg1	properties					52:61	physicochemical properties	36:61	physicochemical properties	36:61	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	7	43	theme	potential	1162:1170	arg1	application					1172:1182	the potential application	1158:1182	the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity	1158:1294	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	4	44	theme	different	763:771	arg1	morphology					781:790	different surface morphology	763:790	different surface morphology	763:790	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	0	45	from	Nostoc	114:119	arg1	activities					79:88	antioxidant activities	67:88	antioxidant activities	67:88	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	45	from	Nostoc	114:119	arg1	polysaccharides					93:107	polysaccharides	93:107	polysaccharides from Nostoc	93:119	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	0	45	from	Nostoc	114:119	arg1	properties					52:61	physicochemical properties	36:61	physicochemical properties	36:61	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	3	46	theme	kDa	681:683	arg1	weights					632:638	the average molecular weights	610:638	the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa	610:683	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	5	47	from	activity	941:948	arg1	ABTS+					900:904	ABTS+	900:904	ABTS+	900:904	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	5	47	from	activity	941:948	arg1	radicals					919:926	hydroxyl radicals	910:926	hydroxyl radicals	910:926	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	5	47	from	activity	941:948	arg1	radical					958:964	DPPH radical	953:964	DPPH radical	953:964	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	2	48	theme	antioxidant	361:371	arg1	activities					373:382	antioxidant activities	361:382	antioxidant activities	361:382	Their physicochemical properties and antioxidant activities were investigated.
30093019	7	49	theme	culture	1208:1214	arg1	conditions					1216:1225	culture conditions	1208:1225	culture conditions	1208:1225	These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
30093019	0	50	theme	antioxidant	67:77	arg1	activities					79:88	antioxidant activities	67:88	antioxidant activities	67:88	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	1	51	dep	stress	268:273	arg1	salt					263:266	normal, salt stress and mixotrophic culture conditions	255:308	salt	263:266	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	3	52	theme	uronic	570:575	arg1	acid					577:580	one uronic acid	566:580	one uronic acid	566:580	WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively.
30093019	1	53	theme	normal	255:260	arg1	stress					268:273	normal, salt stress and mixotrophic culture conditions	255:308	stress	268:273	Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively.
30093019	5	54	theme	Antioxidant	821:831	arg1	assay					833:837	Antioxidant assay	821:837	Antioxidant assay	821:837	Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical.
30093019	6	55	theme	antioxidant	999:1009	arg1	activity					1011:1018	the highest antioxidant activity	987:1018	the highest antioxidant activity	987:1018	Glu-CPS-1 exhibited the highest antioxidant activity suggested culture conditions could regulate the bioactivity through influencing the structure and properties.
30093019	0	56	theme	culture	10:16	arg1	conditions					18:27	culture conditions	10:27	culture conditions	10:27	Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme.
30093019	4	57	dep	fourier	723:729	arg1	spectra					750:756	infrared spectra	741:756	infrared spectra	741:756	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	4	57	dep	fourier	723:729	arg1	transform					731:739	transform	731:739	transform	731:739	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
30093019	4	58	theme	infrared	741:748	arg1	spectra					750:756	infrared spectra	741:756	infrared spectra	741:756	They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching.
31751690	0	0	theme	guar	96:99	arg1	gum					101:103	hydroxypropyl guar gum	82:103	hydroxypropyl guar gum	82:103	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	5	1	theme	response	778:785	arg1	210 ms					793:798	210 ms	793:798	210 ms	793:798	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	1	theme	response	778:785	arg1	time					787:790	fast response time	773:790	fast response time (210 ms)	773:799	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	3	2	theme	biomass	539:545	arg1	hydrogels					547:555	the biomass hydrogels	535:555	the biomass hydrogels	535:555	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	0	3	theme	hydroxypropyl	82:94	arg1	gum					101:103	hydroxypropyl guar gum	82:103	hydroxypropyl guar gum	82:103	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	7	4	theme	application	1073:1083	arg1	prospect					1085:1092	potential application prospect	1063:1092	potential application prospect	1063:1092	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	1	5	theme	self-healing	162:173	arg1	hydrogels					192:200	self-healing, highly flexible hydrogels	162:200	self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG)	162:238	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	6	6	theme	temperature	955:965	arg1	-20-30 °C					974:982	-20-30 °C	974:982	-20-30 °C	974:982	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	6	6	theme	temperature	955:965	arg1	range					967:971	a wide temperature range	948:971	a wide temperature range (-20-30 °C)	948:983	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	3	7	theme	self-healing	484:495	arg1	efficiency					497:506	self-healing efficiency	484:506	self-healing efficiency	484:506	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	7	8	theme	potential	1063:1071	arg1	prospect					1085:1092	potential application prospect	1063:1092	potential application prospect	1063:1092	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	6	9	theme	flexible	815:822	arg1	sensors					824:830	The obtained flexible sensors	802:830	The obtained flexible sensors	802:830	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	6	10	theme	wide	950:953	arg1	-20-30 °C					974:982	-20-30 °C	974:982	-20-30 °C	974:982	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	6	10	theme	wide	950:953	arg1	range					967:971	a wide temperature range	948:971	a wide temperature range (-20-30 °C)	948:983	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	6	11	theme	pulse	891:895	arg1	rates					897:901	pulse rates	891:901	pulse rates	891:901	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	5	12	theme	large	712:716	arg1	range					725:729	a large strain range	710:729	a large strain range (728%)	710:736	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	12	theme	large	712:716	arg1	%					735:735	728%	732:735	728%	732:735	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	6	13	theme	obtained	806:813	arg1	sensors					824:830	The obtained flexible sensors	802:830	The obtained flexible sensors	802:830	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	5	14	theme	sensor	664:669	arg1	hydrogels					695:703	flexible sensor with these self-healing hydrogels	655:703	flexible sensor with these self-healing hydrogels	655:703	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	15	theme	flexible	655:662	arg1	hydrogels					695:703	flexible sensor with these self-healing hydrogels	655:703	flexible sensor with these self-healing hydrogels	655:703	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	16	theme	with	671:674	arg1	hydrogels					695:703	flexible sensor with these self-healing hydrogels	655:703	flexible sensor with these self-healing hydrogels	655:703	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	2	17	theme	boron-oxygen	390:401	arg1	interactions					420:431	boron-oxygen and metal-ligand interactions	390:431	boron-oxygen and metal-ligand interactions	390:431	The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions.
31751690	6	18	theme	sound	870:874	arg1	vibrations					876:885	sound vibrations	870:885	sound vibrations	870:885	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	3	19	theme	multiple	440:447	arg1	networks					449:456	These multiple networks	434:456	These multiple networks	434:456	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	0	20	theme	Self-healing	0:11	arg1	hydrogels					63:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	1	21	theme	dopamine-coated	264:278	arg1	oxide					297:301	dopamine-coated reduced-graphene oxide	264:301	dopamine-coated reduced-graphene oxide (rGO@PDA)	264:311	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	21	theme	dopamine-coated	264:278	arg1	PDA					308:310	rGO@PDA	304:310	rGO@PDA	304:310	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	5	22	theme	self-healing	682:693	arg1	hydrogels					695:703	flexible sensor with these self-healing hydrogels	655:703	flexible sensor with these self-healing hydrogels	655:703	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	7	23	theme	superior	1014:1021	arg1	stability					1023:1031	superior stability	1014:1031	superior stability	1014:1031	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	1	24	dep	self-healing	162:173	arg1	flexible					183:190	flexible	183:190	flexible	183:190	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	7	25	theme	wearable	1119:1126	arg1	devices					1128:1134	flexible wearable devices	1110:1134	flexible wearable devices	1110:1134	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	1	26	theme	reduced-graphene	280:295	arg1	oxide					297:301	dopamine-coated reduced-graphene oxide	264:301	dopamine-coated reduced-graphene oxide (rGO@PDA)	264:311	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	26	theme	reduced-graphene	280:295	arg1	PDA					308:310	rGO@PDA	304:310	rGO@PDA	304:310	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	7	27	contain	have	1009:1012	arg2	repeatability					1037:1049	repeatability	1037:1049	repeatability	1037:1049	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	7	27	contain	have	1009:1012	arg2	stability					1023:1031	superior stability	1014:1031	superior stability	1014:1031	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	7	27	contain	have	1009:1012	arg1	signals					990:996	The signals	986:996	The signals of sensors	986:1007	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	2	28	theme	metal-ligand	407:418	arg1	interactions					420:431	boron-oxygen and metal-ligand interactions	390:431	boron-oxygen and metal-ligand interactions	390:431	The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions.
31751690	0	29	theme	sensitive	14:22	arg1	hydrogels					63:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	0	30	theme	flexible	124:131	arg1	sensors					133:139	flexible sensors	124:139	flexible sensors	124:139	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	2	31	theme	dynamic	338:344	arg1	networks					346:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions.
31751690	7	32	theme	flexible	1110:1117	arg1	devices					1128:1134	flexible wearable devices	1110:1134	flexible wearable devices	1110:1134	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	0	33	theme	biomass	41:47	arg1	hydrogels					63:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	6	34	theme	knee	929:932	arg1	flexion					934:940	knee flexion	929:940	knee flexion	929:940	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	6	35	dep	scales	913:918	arg1	such					921:924	such	921:924	such	921:924	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	1	36	theme	hydroxypropyl	211:223	arg1	HPG					235:237	HPG	235:237	HPG	235:237	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	36	theme	hydroxypropyl	211:223	arg1	gum					230:232	hydroxypropyl guar gum	211:232	hydroxypropyl guar gum (HPG)	211:238	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	0	37	theme	antifreezing	28:39	arg1	hydrogels					63:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	1	38	theme	rGO	304:306	arg1	oxide					297:301	dopamine-coated reduced-graphene oxide	264:301	dopamine-coated reduced-graphene oxide (rGO@PDA)	264:311	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	38	theme	rGO	304:306	arg1	PDA					308:310	rGO@PDA	304:310	rGO@PDA	304:310	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	5	39	theme	high	739:742	arg1	GF = 11.30					757:766	GF = 11.30	757:766	GF = 11.30	757:766	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	39	theme	high	739:742	arg1	sensitivity					744:754	high sensitivity	739:754	high sensitivity (GF = 11.30)	739:767	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	1	40	theme	guar	225:228	arg1	HPG					235:237	HPG	235:237	HPG	235:237	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	40	theme	guar	225:228	arg1	gum					230:232	hydroxypropyl guar gum	211:232	hydroxypropyl guar gum (HPG)	211:238	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	41	theme	@	307:307	arg1	oxide					297:301	dopamine-coated reduced-graphene oxide	264:301	dopamine-coated reduced-graphene oxide (rGO@PDA)	264:311	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	1	41	theme	@	307:307	arg1	PDA					308:310	rGO@PDA	304:310	rGO@PDA	304:310	In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA).
31751690	5	42	theme	strain	718:723	arg1	range					725:729	a large strain range	710:729	a large strain range (728%)	710:736	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	42	theme	strain	718:723	arg1	%					735:735	728%	732:735	728%	732:735	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	4	43	theme	@	583:583	arg1	PDA					584:586	rGO@PDA	580:586	rGO@PDA	580:586	It is noteworthy that rGO@PDA can enhance conductivity and flexibility properties.
31751690	2	44	theme	reversible	327:336	arg1	networks					346:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions.
31751690	0	45	theme	nanocomposite	49:61	arg1	hydrogels					63:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels	0:71	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	4	46	theme	rGO	580:582	arg1	PDA					584:586	rGO@PDA	580:586	rGO@PDA	580:586	It is noteworthy that rGO@PDA can enhance conductivity and flexibility properties.
31751690	7	47	theme	sensors	1001:1007	arg1	signals					990:996	The signals	986:996	The signals of sensors	986:1007	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	6	48	theme	human	848:852	arg1	motion					854:859	human motion	848:859	human motion (such as sound vibrations and pulse rates)	848:902	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	3	49	theme	hydrogels	547:555	arg1	efficiency					497:506	self-healing efficiency	484:506	self-healing efficiency	484:506	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	3	49	theme	hydrogels	547:555	arg1	strength					523:530	mechanical strength	512:530	mechanical strength	512:530	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	2	50	theme	multiple	318:325	arg1	networks					346:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks	314:353	The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions.
31751690	4	51	dep	conductivity	600:611	arg1	properties					629:638	properties	629:638	properties	629:638	It is noteworthy that rGO@PDA can enhance conductivity and flexibility properties.
31751690	0	52	from	application	109:119	arg1	sensors					133:139	flexible sensors	124:139	flexible sensors	124:139	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	3	53	theme	mechanical	512:521	arg1	strength					523:530	mechanical strength	512:530	mechanical strength	512:530	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	0	54	from	gum	101:103	arg1	sensors					133:139	flexible sensors	124:139	flexible sensors	124:139	Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.
31751690	3	55	dep	efficiency	497:506	arg1	the					480:482	the	480:482	the	480:482	These multiple networks significantly improve the self-healing efficiency and mechanical strength of the biomass hydrogels.
31751690	5	56	contain	have	705:708	arg2	sensitivity					744:754	high sensitivity	739:754	high sensitivity (GF = 11.30)	739:767	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg2	%					735:735	728%	732:735	728%	732:735	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg2	210 ms					793:798	210 ms	793:798	210 ms	793:798	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg2	GF = 11.30					757:766	GF = 11.30	757:766	GF = 11.30	757:766	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg1	hydrogels					695:703	flexible sensor with these self-healing hydrogels	655:703	flexible sensor with these self-healing hydrogels	655:703	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg2	time					787:790	fast response time	773:790	fast response time (210 ms)	773:799	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	56	contain	have	705:708	arg2	range					725:729	a large strain range	710:729	a large strain range (728%)	710:736	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	57	theme	fast	773:776	arg1	210 ms					793:798	210 ms	793:798	210 ms	793:798	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	5	57	theme	fast	773:776	arg1	time					787:790	fast response time	773:790	fast response time (210 ms)	773:799	Impressively, flexible sensor with these self-healing hydrogels have a large strain range (728%), high sensitivity (GF = 11.30) and fast response time (210 ms).
31751690	6	58	theme	large	907:911	arg1	scales					913:918	large scales	907:918	large scales (such as knee flexion)	907:941	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
31751690	7	59	theme	devices	1128:1134	arg1	field					1101:1105	the field	1097:1105	the field of flexible wearable devices	1097:1134	The signals of sensors have superior stability and repeatability, which show potential application prospect in the field of flexible wearable devices.
31751690	6	60	dep	motion	854:859	arg1	such					862:865	such	862:865	such	862:865	The obtained flexible sensors can also detect human motion (such as sound vibrations and pulse rates) to large scales (such as knee flexion) over a wide temperature range (-20-30 °C).
29341931	6	0	theme	ca.	960:962	arg1	12 nm					964:968	ca. 12 nm	960:968	ca. 12 nm	960:968	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	8	1	from	decrease	1191:1198	arg1	permeability					1203:1214	permeability	1203:1214	permeability	1203:1214	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	3	2	theme	xyloglucan	516:525	arg1	xyloglucan					516:525	xyloglucan	516:525	xyloglucan	516:525	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	3	2	theme	xyloglucan	516:525	arg1	amount					506:511	a large amount	498:511	a large amount of xyloglucan	498:525	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	1	3	theme	biomedical	280:289	arg1	applications					291:302	biomedical applications	280:302	biomedical applications	280:302	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	11	4	theme	cellulose	1891:1899	arg1	nanofibers					1901:1910	cellulose nanofibers	1891:1910	cellulose nanofibers	1891:1910	The capsules were assembled using pectin, xyloglucan and cellulose in the form of cellulose nanofibers.
29341931	14	5	theme	plant	2414:2418	arg1	cells					2420:2424	natural plant cells	2406:2424	natural plant cells found in vegetables and fruits	2406:2455	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	5	6	theme	live	666:669	arg1	staining					676:683	The live cell staining	662:683	The live cell staining	662:683	The live cell staining revealed that the microcapsules' surface enhanced cell growth and also the non-toxic nature of the microcapsules.
29341931	8	7	theme	capsule-wall	1248:1259	arg1	composition					1261:1271	the capsule-wall composition	1244:1271	the capsule-wall composition	1244:1271	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	7	8	dep	trapping	1119:1126	arg1	fraction					1130:1137	a fraction	1128:1137	trapping a fraction of the 70 kDa dextrans in the capsule interior	1119:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	7	9	theme	250 mM	1097:1102	arg1	trapping					1119:1126	trapping	1119:1126	trapping a fraction of the 70 kDa dextrans in the capsule interior	1119:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	7	9	theme	250 mM	1097:1102	arg1	solution					1109:1116	250 mM NaCl solution	1097:1116	250 mM NaCl solution	1097:1116	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	14	10	theme	cell	2299:2302	arg1	capsules					2304:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	9	11	theme	biological	1430:1439	arg1	medium					1441:1446	a model biological medium	1422:1446	a model biological medium	1422:1446	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	10	12	theme	capsule	1732:1738	arg1	walls					1740:1744	capsule walls	1732:1744	capsule walls that mimic the primary cell wall found in natural plant cells	1732:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	1	13	theme	stimuli-responsive	208:225	arg1	properties					243:252	stimuli-responsive release/loading properties	208:252	stimuli-responsive release/loading properties	208:252	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	10	14	theme	cell	1769:1772	arg1	wall					1774:1777	the primary cell wall	1757:1777	the primary cell wall found in natural plant cells	1757:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	13	15	theme	gastro-intestinal	2257:2273	arg1	tract					2275:2279	the gastro-intestinal tract	2253:2279	the gastro-intestinal tract	2253:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	8	16	theme	direct	1222:1227	arg1	decrease					1191:1198	The decrease	1187:1198	The decrease in permeability	1187:1214	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	16	theme	direct	1222:1227	arg1	consequence					1229:1239	a direct consequence	1220:1239	a direct consequence of the capsule-wall composition	1220:1271	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	6	17	theme	2000 kDa	975:982	arg1	weight					917:922	an average molecular weight	896:922	an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	896:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	2	18	dep	mimic	323:327	arg1	to					320:321	to	320:321	to	320:321	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	11	19	theme	nanofibers	1901:1910	arg1	form					1883:1886	the form	1879:1886	the form of cellulose nanofibers	1879:1910	The capsules were assembled using pectin, xyloglucan and cellulose in the form of cellulose nanofibers.
29341931	7	20	theme	other	1018:1022	arg1	hand					1024:1027	the other hand	1014:1027	the other hand	1014:1027	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	14	21	theme	Such	2282:2285	arg1	capsules					2304:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	5	22	theme	cell	735:738	arg1	growth					740:745	cell growth	735:745	cell growth	735:745	The live cell staining revealed that the microcapsules' surface enhanced cell growth and also the non-toxic nature of the microcapsules.
29341931	13	23	theme	model	2066:2070	arg1	medium					2083:2088	a model biological medium	2064:2088	a model biological medium	2064:2088	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	10	24	theme	natural	1788:1794	arg1	cells					1802:1806	natural plant cells	1788:1806	natural plant cells	1788:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	13	25	theme	targeted	2232:2239	arg1	delivery					2241:2248	colon targeted delivery	2226:2248	colon targeted delivery in the gastro-intestinal tract	2226:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	26	theme	colon	2226:2230	arg1	delivery					2241:2248	colon targeted delivery	2226:2248	colon targeted delivery in the gastro-intestinal tract	2226:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	3	27	theme	first	486:490	arg1	time					492:495	the first time	482:495	the first time	482:495	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	8	28	theme	low	1312:1314	arg1	xyloglucan					1295:1304	xyloglucan	1295:1304	xyloglucan	1295:1304	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	28	theme	low	1312:1314	arg1	amount					1316:1321	a low amount	1310:1321	a low amount of charged molecules such as pectin	1310:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	28	theme	low	1312:1314	arg1	molecules					1334:1342	charged molecules	1326:1342	charged molecules such as pectin	1326:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	28	theme	low	1312:1314	arg1	pectin					1352:1357	pectin	1352:1357	pectin	1352:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	13	29	theme	saline	2038:2043	arg1	conditions					2045:2054	saline conditions	2038:2054	saline conditions	2038:2054	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	30	theme	biological	2193:2202	arg1	applications					2204:2215	biological applications	2193:2215	biological applications	2193:2215	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	30	theme	biological	2193:2202	arg1	delivery					2241:2248	colon targeted delivery	2226:2248	colon targeted delivery in the gastro-intestinal tract	2226:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	11	31	from	cellulose	1866:1874	arg1	form					1883:1886	the form	1879:1886	the form of cellulose nanofibers	1879:1910	The capsules were assembled using pectin, xyloglucan and cellulose in the form of cellulose nanofibers.
29341931	1	32	theme	renewable	158:166	arg1	biopolymers					168:178	renewable biopolymers	158:178	renewable biopolymers	158:178	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	7	33	from	dextrans	1153:1160	arg1	interior					1177:1184	the capsule interior	1165:1184	the capsule interior	1165:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	8	34	theme	molecules	1334:1342	arg1	xyloglucan					1295:1304	xyloglucan	1295:1304	xyloglucan	1295:1304	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	34	theme	molecules	1334:1342	arg1	presence					1283:1290	the presence	1279:1290	the presence of xyloglucan	1279:1304	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	34	theme	molecules	1334:1342	arg1	amount					1316:1321	a low amount	1310:1321	a low amount of charged molecules such as pectin	1310:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	34	theme	molecules	1334:1342	arg1	molecules					1334:1342	charged molecules	1326:1342	charged molecules such as pectin	1326:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	34	theme	molecules	1334:1342	arg1	pectin					1352:1357	pectin	1352:1357	pectin	1352:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	0	35	theme	wall	94:97	arg1	composition					99:109	capsule wall composition	86:109	capsule wall composition	86:109	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	13	36	theme	capsules	2026:2033	arg1	permeability					2010:2021	The low permeability	2002:2021	The low permeability	2002:2021	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	7	37	from	interior	1177:1184	arg1	fraction					1130:1137	a fraction	1128:1137	trapping a fraction of the 70 kDa dextrans in the capsule interior	1119:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	14	38	from	cells	2420:2424	arg1	release					2380:2386	the digestion and release	2362:2386	release	2380:2386	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	14	38	from	cells	2420:2424	arg1	digestion					2366:2374	the digestion and release	2362:2386	digestion	2366:2374	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	2	39	theme	wall	404:407	arg1	polysaccharides					409:423	cell wall polysaccharides	399:423	cell wall polysaccharides	399:423	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	39	theme	wall	404:407	arg1	pectin					433:438	pectin	433:438	pectin	433:438	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	39	theme	wall	404:407	arg1	nanofibers					466:475	xyloglucan and cellulose nanofibers	441:475	xyloglucan and cellulose nanofibers	441:475	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	0	40	theme	Bioinspired	0:10	arg1	capsules					12:19	Bioinspired capsules	0:19	Bioinspired capsules	0:19	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	2	41	theme	cellulose	456:464	arg1	nanofibers					466:475	xyloglucan and cellulose nanofibers	441:475	xyloglucan and cellulose nanofibers	441:475	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	41	theme	cellulose	456:464	arg1	polysaccharides					409:423	cell wall polysaccharides	399:423	cell wall polysaccharides	399:423	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	14	42	located	found	2426:2430	arg2	cells					2420:2424	natural plant cells	2406:2424	natural plant cells found in vegetables and fruits	2406:2455	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	14	42	located	found	2426:2430	arg1	fruits					2450:2455	fruits	2450:2455	fruits	2450:2455	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	14	42	located	found	2426:2430	arg1	vegetables					2435:2444	vegetables	2435:2444	vegetables	2435:2444	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	2	43	theme	xyloglucan	441:450	arg1	nanofibers					466:475	xyloglucan and cellulose nanofibers	441:475	xyloglucan and cellulose nanofibers	441:475	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	43	theme	xyloglucan	441:450	arg1	polysaccharides					409:423	cell wall polysaccharides	399:423	cell wall polysaccharides	399:423	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	11	44	from	xyloglucan	1851:1860	arg1	form					1883:1886	the form	1879:1886	the form of cellulose nanofibers	1879:1910	The capsules were assembled using pectin, xyloglucan and cellulose in the form of cellulose nanofibers.
29341931	7	45	theme	dextrans	1153:1160	arg1	fraction					1130:1137	a fraction	1128:1137	trapping a fraction of the 70 kDa dextrans in the capsule interior	1119:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	13	46	theme	dietary	2139:2145	arg1	fibers					2147:2152	dietary fibers	2139:2152	dietary fibers only	2139:2157	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	47	theme	average	899:905	arg1	weight					917:922	an average molecular weight	896:922	an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	896:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	9	48	theme	biological	1551:1560	arg1	applications					1562:1573	biological applications	1551:1573	biological applications	1551:1573	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	48	theme	biological	1551:1560	arg1	delivery					1599:1606	colon targeted delivery	1584:1606	colon targeted delivery in the gastro-intestinal tract	1584:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	49	with	dextrans	882:889	arg1	weight					917:922	an average molecular weight	896:922	an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	896:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	14	50	theme	natural	2406:2412	arg1	cells					2420:2424	natural plant cells	2406:2424	natural plant cells found in vegetables and fruits	2406:2455	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	10	51	theme	first	1674:1678	arg1	time					1680:1683	the first time	1670:1683	the first time	1670:1683	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	7	52	from	fraction	1130:1137	arg1	interior					1177:1184	the capsule interior	1165:1184	the capsule interior	1165:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	13	53	theme	low	2006:2008	arg1	permeability					2010:2021	The low permeability	2002:2021	The low permeability	2002:2021	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	54	theme	fluorescent	870:880	arg1	dextrans					882:889	fluorescent dextrans	870:889	fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	870:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	8	55	dep	presence	1283:1290	arg1	i.e.					1274:1277	i.e.	1274:1277	i.e.	1274:1277	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	9	56	theme	capsules	1384:1391	arg1	permeability					1368:1379	The low permeability	1360:1379	The low permeability	1360:1379	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	57	theme	12 nm	964:968	arg1	diameter					948:955	hydrodynamic diameter	935:955	hydrodynamic diameter of ca. 12 nm	935:968	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	9	58	theme	targeted	1590:1597	arg1	delivery					1599:1606	colon targeted delivery	1584:1606	colon targeted delivery in the gastro-intestinal tract	1584:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	59	theme	saline	1396:1401	arg1	conditions					1403:1412	saline conditions	1396:1412	saline conditions	1396:1412	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	4	60	theme	stimuli-responsive	599:616	arg1	permeability					627:638	permeability	627:638	permeability	627:638	These capsules demonstrated stimuli-responsive (ON/OFF) permeability and biocompatibility.
29341931	3	61	theme	such	556:559	arg1	capsules					561:568	such capsules	556:568	such capsules	556:568	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	13	62	theme	capsule	2108:2114	arg1	wall					2116:2119	a capsule wall	2106:2119	a capsule wall that is made from dietary fibers only	2106:2157	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	63	theme	hydrodynamic	935:946	arg1	diameter					948:955	hydrodynamic diameter	935:955	hydrodynamic diameter of ca. 12 nm	935:968	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	9	64	theme	gastro-intestinal	1615:1631	arg1	tract					1633:1637	the gastro-intestinal tract	1611:1637	the gastro-intestinal tract	1611:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	3	65	theme	large	500:504	arg1	xyloglucan					516:525	xyloglucan	516:525	xyloglucan	516:525	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	3	65	theme	large	500:504	arg1	amount					506:511	a large amount	498:511	a large amount of xyloglucan	498:525	For the first time, a large amount of xyloglucan was successfully included in such capsules.
29341931	7	66	theme	NaCl	1104:1107	arg1	trapping					1119:1126	trapping	1119:1126	trapping a fraction of the 70 kDa dextrans in the capsule interior	1119:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	7	66	theme	NaCl	1104:1107	arg1	solution					1109:1116	250 mM NaCl solution	1097:1116	250 mM NaCl solution	1097:1116	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	1	67	theme	selective	181:189	arg1	permeability					191:202	selective permeability	181:202	selective permeability	181:202	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	6	68	theme	70 kDa	927:932	arg1	weight					917:922	an average molecular weight	896:922	an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	896:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	5	69	theme	cell	671:674	arg1	staining					676:683	The live cell staining	662:683	The live cell staining	662:683	The live cell staining revealed that the microcapsules' surface enhanced cell growth and also the non-toxic nature of the microcapsules.
29341931	2	70	theme	plant	333:337	arg1	cell-wall					347:355	the plant primary cell-wall	329:355	the plant primary cell-wall	329:355	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	9	71	theme	model	1424:1428	arg1	medium					1441:1446	a model biological medium	1422:1446	a model biological medium	1422:1446	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	72	from	permeability	2010:2021	arg1	conditions					2045:2054	saline conditions	2038:2054	saline conditions	2038:2054	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	72	from	permeability	2010:2021	arg1	medium					2083:2088	a model biological medium	2064:2088	a model biological medium	2064:2088	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	0	73	theme	permeability	114:125	arg1	properties					127:136	permeability properties	114:136	permeability properties	114:136	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	14	74	theme	plant	2293:2297	arg1	capsules					2304:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	12	75	theme	stimuli-responsive	1939:1956	arg1	permeability					1967:1978	permeability	1967:1978	permeability	1967:1978	The capsules demonstrated stimuli-responsive (ON/OFF) permeability and biocompatibility.
29341931	0	76	from	influence	73:81	arg1	properties					127:136	permeability properties	114:136	permeability properties	114:136	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	8	77	theme	composition	1261:1271	arg1	decrease					1191:1198	The decrease	1187:1198	The decrease in permeability	1187:1214	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	77	theme	composition	1261:1271	arg1	consequence					1229:1239	a direct consequence	1220:1239	a direct consequence of the capsule-wall composition	1220:1271	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	1	78	theme	release/loading	227:241	arg1	properties					243:252	stimuli-responsive release/loading properties	208:252	stimuli-responsive release/loading properties	208:252	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	5	79	theme	non-toxic	760:768	arg1	nature					770:775	the non-toxic nature	756:775	the non-toxic nature of the microcapsules	756:796	The live cell staining revealed that the microcapsules' surface enhanced cell growth and also the non-toxic nature of the microcapsules.
29341931	4	80	dep	stimuli-responsive	599:616	arg1	ON/OFF					619:624	ON/OFF	619:624	ON/OFF	619:624	These capsules demonstrated stimuli-responsive (ON/OFF) permeability and biocompatibility.
29341931	10	81	theme	primary	1761:1767	arg1	wall					1774:1777	the primary cell wall	1757:1777	the primary cell wall found in natural plant cells	1757:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	9	82	from	delivery	1599:1606	arg1	tract					1633:1637	the gastro-intestinal tract	1611:1637	the gastro-intestinal tract	1611:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	83	dep	54 nm	989:993	arg1	ca.					985:987	ca.	985:987	ca.	985:987	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	14	84	theme	model	2287:2291	arg1	capsules					2304:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules	2282:2311	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	10	85	theme	plant	1796:1800	arg1	cells					1802:1806	natural plant cells	1788:1806	natural plant cells	1788:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	9	86	theme	capsule	1466:1472	arg1	wall					1474:1477	a capsule wall	1464:1477	a capsule wall that is made from dietary fibers only	1464:1515	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	87	from	use	1544:1546	arg1	applications					1562:1573	biological applications	1551:1573	biological applications	1551:1573	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	87	from	use	1544:1546	arg1	delivery					1599:1606	colon targeted delivery	1584:1606	colon targeted delivery in the gastro-intestinal tract	1584:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	8	88	theme	xyloglucan	1295:1304	arg1	xyloglucan					1295:1304	xyloglucan	1295:1304	xyloglucan	1295:1304	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	88	theme	xyloglucan	1295:1304	arg1	presence					1283:1290	the presence	1279:1290	the presence of xyloglucan	1279:1304	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	88	theme	xyloglucan	1295:1304	arg1	amount					1316:1321	a low amount	1310:1321	a low amount of charged molecules such as pectin	1310:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	88	theme	xyloglucan	1295:1304	arg1	molecules					1334:1342	charged molecules	1326:1342	charged molecules such as pectin	1326:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	88	theme	xyloglucan	1295:1304	arg1	pectin					1352:1357	pectin	1352:1357	pectin	1352:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	5	89	theme	microcapsules	784:796	arg1	nature					770:775	the non-toxic nature	756:775	the non-toxic nature of the microcapsules	756:796	The live cell staining revealed that the microcapsules' surface enhanced cell growth and also the non-toxic nature of the microcapsules.
29341931	13	90	theme	biological	2072:2081	arg1	medium					2083:2088	a model biological medium	2064:2088	a model biological medium	2064:2088	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	10	91	contain	possess	1724:1730	arg1	that					1719:1722	that	1719:1722	that	1719:1722	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	10	91	contain	possess	1724:1730	arg2	walls					1740:1744	capsule walls	1732:1744	capsule walls that mimic the primary cell wall found in natural plant cells	1732:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	0	92	theme	capsule	86:92	arg1	composition					99:109	capsule wall composition	86:109	capsule wall composition	86:109	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	9	93	theme	dietary	1497:1503	arg1	fibers					1505:1510	dietary fibers	1497:1510	dietary fibers only	1497:1515	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	8	94	theme	charged	1326:1332	arg1	molecules					1334:1342	charged molecules	1326:1342	charged molecules such as pectin	1326:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	8	94	theme	charged	1326:1332	arg1	pectin					1352:1357	pectin	1352:1357	pectin	1352:1357	The decrease in permeability was a direct consequence of the capsule-wall composition, i.e. the presence of xyloglucan and a low amount of charged molecules such as pectin.
29341931	2	95	theme	primary	339:345	arg1	cell-wall					347:355	the plant primary cell-wall	329:355	the plant primary cell-wall	329:355	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	0	96	theme	composition	99:109	arg1	influence					73:81	The influence	69:81	The influence of capsule wall composition on permeability properties	69:136	Bioinspired capsules based on nanocellulose, xyloglucan and pectin - The influence of capsule wall composition on permeability properties.
29341931	12	97	dep	stimuli-responsive	1939:1956	arg1	ON/OFF					1959:1964	ON/OFF	1959:1964	ON/OFF	1959:1964	The capsules demonstrated stimuli-responsive (ON/OFF) permeability and biocompatibility.
29341931	7	98	theme	capsule	1169:1175	arg1	interior					1177:1184	the capsule interior	1165:1184	the capsule interior	1165:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	6	99	from	permeable	857:865	arg1	water					802:806	water	802:806	water	802:806	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	2	100	theme	cell	399:402	arg1	polysaccharides					409:423	cell wall polysaccharides	399:423	cell wall polysaccharides	399:423	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	100	theme	cell	399:402	arg1	pectin					433:438	pectin	433:438	pectin	433:438	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	2	100	theme	cell	399:402	arg1	nanofibers					466:475	xyloglucan and cellulose nanofibers	441:475	xyloglucan and cellulose nanofibers	441:475	Here, in order to mimic the plant primary cell-wall, microcapsules have been fabricated using cell wall polysaccharides, namely pectin, xyloglucan and cellulose nanofibers.
29341931	10	101	located	found	1779:1783	arg1	cells					1802:1806	natural plant cells	1788:1806	natural plant cells	1788:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	10	101	located	found	1779:1783	arg2	wall					1774:1777	the primary cell wall	1757:1777	the primary cell wall found in natural plant cells	1757:1806	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	7	102	theme	70 kDa	1146:1151	arg1	dextrans					1153:1160	the 70 kDa dextrans	1142:1160	the 70 kDa dextrans in the capsule interior	1142:1184	On the other hand, the permeability dropped quickly when the capsules were exposed to 250 mM NaCl solution, trapping a fraction of the 70 kDa dextrans in the capsule interior.
29341931	6	103	theme	molecular	907:915	arg1	weight					917:922	an average molecular weight	896:922	an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively	896:1008	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	1	104	theme	important	262:270	arg1	role					272:275	an important role	259:275	an important role	259:275	Materials based on renewable biopolymers, selective permeability and stimuli-responsive release/loading properties play an important role in biomedical applications.
29341931	14	105	theme	nutrients	2391:2399	arg1	release					2380:2386	the digestion and release	2362:2386	release	2380:2386	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	14	105	theme	nutrients	2391:2399	arg1	digestion					2366:2374	the digestion and release	2362:2386	digestion	2366:2374	Such model plant cell capsules might also further improve the understanding for the digestion and release of nutrients from natural plant cells found in vegetables and fruits.
29341931	13	106	from	use	2186:2188	arg1	applications					2204:2215	biological applications	2193:2215	biological applications	2193:2215	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	106	from	use	2186:2188	arg1	delivery					2241:2248	colon targeted delivery	2226:2248	colon targeted delivery in the gastro-intestinal tract	2226:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	13	107	from	delivery	2241:2248	arg1	tract					2275:2279	the gastro-intestinal tract	2253:2279	the gastro-intestinal tract	2253:2279	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	108	theme	low	1364:1366	arg1	permeability					1368:1379	The low permeability	1360:1379	The low permeability	1360:1379	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	109	from	permeability	1368:1379	arg1	conditions					1403:1412	saline conditions	1396:1412	saline conditions	1396:1412	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	9	109	from	permeability	1368:1379	arg1	medium					1441:1446	a model biological medium	1422:1446	a model biological medium	1422:1446	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29341931	6	110	from	water	802:806	arg1	microcapsules					813:825	the microcapsules	809:825	the microcapsules	809:825	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	6	110	from	water	802:806	arg1	permeable					857:865	permeable	857:865	permeable	857:865	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	6	111	dep	70 kDa	927:932	arg1	diameter					948:955	hydrodynamic diameter	935:955	hydrodynamic diameter of ca. 12 nm	935:968	In water, the microcapsules were completely and partially permeable to fluorescent dextrans with an average molecular weight of 70 kDa (hydrodynamic diameter of ca. 12 nm) and 2000 kDa (ca. 54 nm), respectively.
29341931	10	112	theme	SIGNIFICANCE	1653:1664	arg1	STATEMENT					1640:1648	STATEMENT	1640:1648	STATEMENT	1640:1648	STATEMENT OF SIGNIFICANCE For the first time, microcapsules have been prepared that possess capsule walls that mimic the primary cell wall found in natural plant cells.
29341931	9	113	theme	colon	1584:1588	arg1	delivery					1599:1606	colon targeted delivery	1584:1606	colon targeted delivery in the gastro-intestinal tract	1584:1637	The low permeability of capsules in saline conditions (and in a model biological medium), combined with a capsule wall that is made from dietary fibers only, potentially enables their use in biological applications, such as colon targeted delivery in the gastro-intestinal tract.
29489058	10	0	theme	A2Sr5	1597:1601	arg1	glasses					1613:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	12	1	theme	mineralization	1866:1879	arg1	extent					1856:1861	The extent	1852:1861	The extent of mineralization	1852:1879	The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5.
29489058	12	1	theme	mineralization	1866:1879	arg1	greatest					1896:1903	greatest	1896:1903	greatest	1896:1903	The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5.
29489058	6	2	theme	individual	938:947	arg1	microparticles					949:962	individual microparticles	938:962	individual microparticles	938:962	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	13	3	dep	injectable	2032:2041	arg1	self-gelling					2044:2055	self-gelling	2044:2055	self-gelling	2044:2055	The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
29489058	9	4	theme	bioactive	1450:1458	arg1	preparations					1466:1477	all four bioactive glass preparations	1441:1477	all four bioactive glass preparations	1441:1477	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	10	5	theme	cells	1674:1678	arg1	adhesion					1635:1642	adhesion	1635:1642	adhesion	1635:1642	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	10	5	theme	cells	1674:1678	arg1	growth					1648:1653	growth	1648:1653	growth	1648:1653	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	2	6	theme	gum	338:340	arg1	hydrogels					347:355	gellan gum (GG) hydrogels	331:355	gellan gum (GG) hydrogels	331:355	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	1	7	theme	hydrogel	216:223	arg1	biomaterials					225:236	hydrogel biomaterials	216:236	hydrogel biomaterials	216:236	Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration.
29489058	6	8	theme	microparticles	1033:1046	arg1	distribution					1001:1012	the size distribution	992:1012	the size distribution of bioactive glass microparticles and their aggregates	992:1067	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	8	theme	microparticles	1033:1046	arg1	distribution					1080:1091	their distribution	1074:1091	their distribution in GG hydrogels	1074:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	5	9	theme	glass	744:748	arg1	preparation					750:760	the standard 45S5 bioactive glass preparation	716:760	the standard 45S5 bioactive glass preparation	716:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	3	10	theme	injectable	577:586	arg1	composites					602:611	novel injectable, self-gelling composites	571:611	novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass	571:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	10	theme	injectable	577:586	arg1	hydrogels					619:627	GG hydrogels	616:627	GG hydrogels containing 20% bioactive glass	616:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	9	11	theme	antibacterial	1489:1501	arg1	activity					1503:1510	antibacterial activity	1489:1510	antibacterial activity against methicillin-resistant Staphylococcus aureus	1489:1562	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	6	12	theme	bioactive	1017:1025	arg1	microparticles					1033:1046	bioactive glass microparticles	1017:1046	bioactive glass microparticles	1017:1046	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	7	13	theme	melt-derived	1126:1137	arg1	standard					1168:1175	standard	1168:1175	standard	1168:1175	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	7	13	theme	melt-derived	1126:1137	arg1	preparation					1144:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	3	14	theme	hydrogels	619:627	arg1	composites					602:611	novel injectable, self-gelling composites	571:611	novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass	571:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	14	theme	hydrogels	619:627	arg1	hydrogels					619:627	GG hydrogels	616:627	GG hydrogels containing 20% bioactive glass	616:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	13	15	theme	injectable	2032:2041	arg1	composites					2057:2066	injectable, self-gelling composites	2032:2066	injectable, self-gelling composites	2032:2066	The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
29489058	5	16	theme	45S5	729:732	arg1	preparation					750:760	the standard 45S5 bioactive glass preparation	716:760	the standard 45S5 bioactive glass preparation	716:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	3	17	theme	bioactive	644:652	arg1	glass					654:658	20% bioactive glass	640:658	20% bioactive glass	640:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	18	theme	20	640:641	arg1	%					642:642	%	642:642	%	642:642	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	10	19	contain	containing	1576:1585	arg1	Composites					1565:1574	Composites	1565:1574	Composites containing A2Zn5 and A2Sr5 bioactive glasses	1565:1619	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	10	19	contain	containing	1576:1585	arg2	glasses					1613:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	6	20	from	distribution	1080:1091	arg1	hydrogels					1099:1107	GG hydrogels	1096:1107	GG hydrogels	1096:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	8	21	theme	A2Sr	1327:1330	arg1	particles					1348:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	13	22	theme	bioactive	2001:2009	arg1	glass					2011:2015	bioactive glass	2001:2015	bioactive glass	2001:2015	The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
29489058	0	23	theme	bioactive	82:90	arg1	microparticles					98:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	8	24	theme	glass	1342:1346	arg1	particles					1348:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	2	25	theme	bioactive	522:530	arg1	microparticles					538:551	the amorphous bioactive glass microparticles	508:551	the amorphous bioactive glass microparticles	508:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	26	theme	High-resolution	114:128	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	26	theme	High-resolution	114:128	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	11	27	from	incubation	1816:1825	arg1	fluid					1845:1849	simulated body fluid	1830:1849	simulated body fluid	1830:1849	All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid.
29489058	13	28	theme	choice	1991:1996	arg1	importance					1973:1982	the importance	1969:1982	the importance of the choice of bioactive glass	1969:2015	The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
29489058	2	29	theme	GG	389:390	arg1	solution					392:399	GG solution	389:399	GG solution	389:399	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	30	theme	Novel	0:4	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	30	theme	Novel	0:4	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	30	theme	Novel	0:4	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	30	theme	Novel	0:4	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	10	31	dep	adhesion	1635:1642	arg1	the					1631:1633	the	1631:1633	the	1631:1633	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	2	32	theme	composition	445:455	arg1	microparticles					422:435	bioactive glass microparticles	406:435	bioactive glass microparticles of 45S5 composition	406:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	33	theme	gellan	17:22	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	33	theme	gellan	17:22	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	33	theme	gellan	17:22	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	33	theme	gellan	17:22	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	2	34	theme	glass	416:420	arg1	microparticles					422:435	bioactive glass microparticles	406:435	bioactive glass microparticles of 45S5 composition	406:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	35	theme	hydrogel	28:35	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	35	theme	hydrogel	28:35	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	35	theme	hydrogel	28:35	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	35	theme	hydrogel	28:35	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	2	36	theme	hydrogel	469:476	arg1	formation					478:486	hydrogel formation	469:486	hydrogel formation by ion release from the amorphous bioactive glass microparticles	469:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	7	37	theme	calcium-rich	1201:1212	arg1	preparation					1231:1241	a calcium-rich, sol-gel derived preparation	1199:1241	a calcium-rich, sol-gel derived preparation (A2)	1199:1246	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	7	37	theme	calcium-rich	1201:1212	arg1	A2					1244:1245	A2	1244:1245	A2	1244:1245	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	6	38	theme	diameter	900:907	arg1	microparticles					882:895	microparticles	882:895	microparticles	882:895	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	5	39	contain	containing	705:714	arg2	preparation					750:760	the standard 45S5 bioactive glass preparation	716:760	the standard 45S5 bioactive glass preparation	716:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	5	39	contain	containing	705:714	arg1	Composites					694:703	Composites	694:703	Composites containing the standard 45S5 bioactive glass preparation	694:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	6	40	theme	X-ray	788:792	arg1	technique					851:859	a highly sensitive technique	832:859	a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels	832:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	40	theme	X-ray	788:792	arg1	tomography					808:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	2	41	with	mixing	379:384	arg1	microparticles					422:435	bioactive glass microparticles	406:435	bioactive glass microparticles of 45S5 composition	406:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	3	42	dep	injectable	577:586	arg1	self-gelling					589:600	self-gelling	589:600	self-gelling	589:600	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	43	contain	containing	629:638	arg2	glass					654:658	20% bioactive glass	640:658	20% bioactive glass	640:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	43	contain	containing	629:638	arg1	hydrogels					619:627	GG hydrogels	616:627	GG hydrogels containing 20% bioactive glass	616:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	11	44	theme	simulated	1830:1838	arg1	fluid					1845:1849	simulated body fluid	1830:1849	simulated body fluid	1830:1849	All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid.
29489058	10	45	theme	bioactive	1603:1611	arg1	glasses					1613:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	7	46	dep	derived	1223:1229	arg1	sol-gel					1215:1221	sol-gel	1215:1221	sol-gel	1215:1221	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	6	47	theme	capable	861:867	arg1	technique					851:859	a highly sensitive technique	832:859	a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels	832:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	47	theme	capable	861:867	arg1	tomography					808:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	1	48	theme	bone	297:300	arg1	regeneration					302:313	bone regeneration	297:313	bone regeneration	297:313	Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration.
29489058	6	49	theme	sensitive	841:849	arg1	technique					851:859	a highly sensitive technique	832:859	a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels	832:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	49	theme	sensitive	841:849	arg1	tomography					808:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	9	50	contain	containing	1430:1439	arg1	Composites					1419:1428	Composites	1419:1428	Composites containing all four bioactive glass preparations	1419:1477	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	9	50	contain	containing	1430:1439	arg2	preparations					1466:1477	all four bioactive glass preparations	1441:1477	all four bioactive glass preparations	1441:1477	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	9	51	theme	glass	1460:1464	arg1	preparations					1466:1477	all four bioactive glass preparations	1441:1477	all four bioactive glass preparations	1441:1477	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	10	52	theme	osteoblast-like	1658:1672	arg1	cells					1674:1678	osteoblast-like cells	1658:1678	osteoblast-like cells	1658:1678	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	12	53	contain	containing	1920:1929	arg1	composites					1909:1918	composites	1909:1918	composites containing A2Zn5 and 45S5	1909:1944	The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5.
29489058	12	53	contain	containing	1920:1929	arg2	45S5					1941:1944	45S5	1941:1944	45S5	1941:1944	The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5.
29489058	12	53	contain	containing	1920:1929	arg2	A2Zn5					1931:1935	A2Zn5	1931:1935	A2Zn5	1931:1935	The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5.
29489058	5	54	theme	bioactive	734:742	arg1	preparation					750:760	the standard 45S5 bioactive glass preparation	716:760	the standard 45S5 bioactive glass preparation	716:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	2	55	theme	gellan	331:336	arg1	GG					343:344	GG	343:344	GG	343:344	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	2	55	theme	gellan	331:336	arg1	gum					338:340	gellan gum	331:340	gellan gum (GG) hydrogels	331:355	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	1	56	theme	biomaterials	225:236	arg1	Mineralization					198:211	Mineralization	198:211	Mineralization of hydrogel biomaterials	198:236	Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration.
29489058	6	57	theme	glass	1027:1031	arg1	microparticles					1033:1046	bioactive glass microparticles	1017:1046	bioactive glass microparticles	1017:1046	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	7	58	theme	used	1121:1124	arg1	standard					1168:1175	standard	1168:1175	standard	1168:1175	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	7	58	theme	used	1121:1124	arg1	preparation					1144:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	3	59	theme	GG	616:617	arg1	hydrogels					619:627	GG hydrogels	616:627	GG hydrogels containing 20% bioactive glass	616:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	0	60	theme	in	180:181	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	60	theme	in	180:181	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	6	61	theme	size	996:999	arg1	distribution					1001:1012	the size distribution	992:1012	the size distribution of bioactive glass microparticles and their aggregates	992:1067	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	7	62	theme	45S5	1139:1142	arg1	standard					1168:1175	standard	1168:1175	standard	1168:1175	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	7	62	theme	45S5	1139:1142	arg1	preparation					1144:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	5	63	theme	standard	720:727	arg1	preparation					750:760	the standard 45S5 bioactive glass preparation	716:760	the standard 45S5 bioactive glass preparation	716:760	Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff.
29489058	3	64	theme	%	642:642	arg1	glass					654:658	20% bioactive glass	640:658	20% bioactive glass	640:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	0	65	dep	in	180:181	arg1	vitro					183:187	vitro	183:187	vitro	183:187	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	3	66	theme	novel	571:575	arg1	composites					602:611	novel injectable, self-gelling composites	571:611	novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass	571:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	3	66	theme	novel	571:575	arg1	hydrogels					619:627	GG hydrogels	616:627	GG hydrogels containing 20% bioactive glass	616:658	This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass.
29489058	6	67	theme	GG	1096:1097	arg1	hydrogels					1099:1107	GG hydrogels	1096:1107	GG hydrogels	1096:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	2	68	from	microparticles	538:551	arg1	release					495:501	ion release	491:501	ion release from the amorphous bioactive glass microparticles	491:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	8	69	theme	A2	1313:1314	arg1	particles					1348:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	0	70	theme	Sr-enriched	70:80	arg1	microparticles					98:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	8	71	theme	A2Zn	1317:1320	arg1	particles					1348:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	0	72	theme	glass	92:96	arg1	microparticles					98:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	2	73	theme	ion	491:493	arg1	release					495:501	ion release	491:501	ion release from the amorphous bioactive glass microparticles	491:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	13	74	theme	glass	2011:2015	arg1	choice					1991:1996	the choice	1987:1996	the choice of bioactive glass	1987:2015	The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
29489058	6	75	theme	aggregates	1058:1067	arg1	distribution					1001:1012	the size distribution	992:1012	the size distribution of bioactive glass microparticles and their aggregates	992:1067	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	75	theme	aggregates	1058:1067	arg1	distribution					1080:1091	their distribution	1074:1091	their distribution in GG hydrogels	1074:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	2	76	theme	glass	532:536	arg1	microparticles					538:551	the amorphous bioactive glass microparticles	508:551	the amorphous bioactive glass microparticles	508:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	8	77	theme	bioactive	1332:1340	arg1	particles					1348:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles	1313:1356	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	0	78	theme	X-ray	130:134	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	78	theme	X-ray	130:134	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	2	79	theme	amorphous	512:520	arg1	microparticles					538:551	the amorphous bioactive glass microparticles	508:551	the amorphous bioactive glass microparticles	508:551	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	7	80	dep	calcium-rich	1201:1212	arg1	derived					1223:1229	derived	1223:1229	derived	1223:1229	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	9	81	dep	Staphylococcus	1542:1555	arg1	aureus					1557:1562	aureus	1557:1562	aureus	1557:1562	Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus.
29489058	2	82	theme	solution	392:399	arg1	mixing					379:384	simple mixing	372:384	simple mixing of GG solution with bioactive glass microparticles of 45S5 composition	372:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	83	theme	injectable	6:15	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	83	theme	injectable	6:15	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	83	theme	injectable	6:15	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	83	theme	injectable	6:15	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	84	theme	gum	24:26	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	84	theme	gum	24:26	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	84	theme	gum	24:26	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	84	theme	gum	24:26	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	2	85	theme	45S5	440:443	arg1	composition					445:455	45S5 composition	440:455	45S5 composition	440:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	11	86	theme	calcium-deficient	1778:1794	arg1	hydroxyapatite					1796:1809	calcium-deficient hydroxyapatite	1778:1809	calcium-deficient hydroxyapatite	1778:1809	All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid.
29489058	8	87	theme	GG	1395:1396	arg1	hydrogels					1398:1406	GG hydrogels	1395:1406	GG hydrogels than 45S5	1395:1416	A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5.
29489058	2	88	theme	bioactive	406:414	arg1	microparticles					422:435	bioactive glass microparticles	406:435	bioactive glass microparticles of 45S5 composition	406:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	0	89	theme	Zn-	62:64	arg1	microparticles					98:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Zn- and Sr-enriched bioactive glass microparticles	62:111	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	90	dep	composites	37:46	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	90	dep	composites	37:46	arg1	antibacterial					162:174	antibacterial	162:174	antibacterial	162:174	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	90	dep	composites	37:46	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	90	dep	composites	37:46	arg1	testing					189:195	in vitro testing	180:195	in vitro testing	180:195	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	10	91	theme	A2Zn5	1587:1591	arg1	glasses					1613:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	A2Zn5 and A2Sr5 bioactive glasses	1587:1619	Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5.
29489058	11	92	theme	body	1840:1843	arg1	fluid					1845:1849	simulated body fluid	1830:1849	simulated body fluid	1830:1849	All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid.
29489058	6	93	theme	microcomputed	794:806	arg1	technique					851:859	a highly sensitive technique	832:859	a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels	832:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	93	theme	microcomputed	794:806	arg1	tomography					808:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography	788:817	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	6	94	from	distribution	1001:1012	arg1	hydrogels					1099:1107	GG hydrogels	1096:1107	GG hydrogels	1096:1107	X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels.
29489058	2	95	theme	simple	372:377	arg1	mixing					379:384	simple mixing	372:384	simple mixing of GG solution with bioactive glass microparticles of 45S5 composition	372:455	In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles.
29489058	11	96	with	mineralization	1758:1771	arg1	hydroxyapatite					1796:1809	calcium-deficient hydroxyapatite	1778:1809	calcium-deficient hydroxyapatite	1778:1809	All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid.
29489058	0	97	theme	microcomputed	136:148	arg1	composites					37:46	Novel injectable gellan gum hydrogel composites	0:46	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.	0:196	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	0	97	theme	microcomputed	136:148	arg1	tomography					150:159	High-resolution X-ray microcomputed tomography	114:159	High-resolution X-ray microcomputed tomography	114:159	Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing.
29489058	7	98	link	melt-derived	1126:1137	arg1	standard					1168:1175	standard	1168:1175	standard	1168:1175	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29489058	7	98	link	melt-derived	1126:1137	arg1	preparation					1144:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation	1110:1154	The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5).
29397980	4	0	theme	methanol	925:932	arg1	eluents					940:946	methanol based eluents	925:946	methanol based eluents at different isocratic compositions	925:982	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	6	1	theme	package	1421:1427	arg1	preparation					1429:1439	technical package preparation	1411:1439	technical package preparation	1411:1439	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	2	theme	technical	529:537	arg1	package					539:545	The analytical technical package	514:545	The analytical technical package of validated methods	514:566	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	5	3	gly	glycopeptide	1136:1147	arg2	glycopeptide					1136:1147	another macrocyclic glycopeptide chiral selector	1116:1163	another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles	1116:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	1	4	theme	clinical	258:265	arg1	trials					267:272	clinical trials	258:272	clinical trials for the treatment of Alzheimer's disease	258:313	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	6	5	theme	manufacturing	1476:1488	arg1	facilities					1490:1499	manufacturing facilities	1476:1499	manufacturing facilities	1476:1499	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	1	6	theme	β-site	180:185	arg1	protein					205:211	β-site amyloid precursor protein	180:211	β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease	180:313	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	5	7	theme	verubecestat	1069:1080	arg1	determination					1052:1064	EE determination	1049:1064	EE determination of verubecestat	1049:1080	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	0	8	theme	entire	111:116	arg1	route					141:145	the entire verubecestat synthetic route	107:145	the entire verubecestat synthetic route	107:145	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	4	9	theme	bonded	834:839	arg1	Teicoplanin					821:831	Teicoplanin	821:831	Teicoplanin	821:831	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	9	theme	bonded	834:839	arg1	selector					811:818	a single chiral selector	795:818	a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate	795:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	6	10	theme	entire	1284:1289	arg1	route					1314:1318	the entire verubecestat synthetic route	1280:1318	the entire verubecestat synthetic route using only two chiral columns	1280:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	5	11	theme	EE	1049:1050	arg1	determination					1052:1064	EE determination	1049:1064	EE determination of verubecestat	1049:1080	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	6	12	theme	enantiopurity	1254:1266	arg1	analysis					1268:1275	fast and reliable enantiopurity analysis	1236:1275	fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns	1236:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	13	theme	RPLC	603:606	arg1	separations					608:618	enantioselective SFC and RPLC separations	578:618	separations	608:618	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	4	14	theme	based	934:938	arg1	eluents					940:946	methanol based eluents	925:946	methanol based eluents at different isocratic compositions	925:982	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	3	15	theme	enantioselective	578:593	arg1	SFC					595:597	enantioselective SFC and RPLC separations	578:618	SFC	595:597	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	4	16	theme	chiral	771:776	arg1	columns					778:784	recently developed chiral columns	752:784	recently developed chiral columns	752:784	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	0	17	theme	synthetic	131:139	arg1	route					141:145	the entire verubecestat synthetic route	107:145	the entire verubecestat synthetic route	107:145	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	6	18	theme	reliable	1245:1252	arg1	analysis					1268:1275	fast and reliable enantiopurity analysis	1236:1275	fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns	1236:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	6	19	theme	HPLC	1386:1389	arg1	system					1391:1396	a conventional HPLC system	1371:1396	a conventional HPLC system	1371:1396	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	20	theme	methods	560:566	arg1	package					539:545	The analytical technical package	514:545	The analytical technical package of validated methods	514:566	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	1	21	theme	protein	205:211	arg1	Verubecestat					148:159	Verubecestat	148:159	Verubecestat	148:159	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	1	21	theme	protein	205:211	arg1	inhibitor					167:175	an inhibitor	164:175	an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease	164:313	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	1	22	theme	amyloid	187:193	arg1	protein					205:211	β-site amyloid precursor protein	180:211	β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease	180:313	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	0	23	theme	verubecestat	118:129	arg1	route					141:145	the entire verubecestat synthetic route	107:145	the entire verubecestat synthetic route	107:145	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	6	24	theme	conventional	1373:1384	arg1	system					1391:1396	a conventional HPLC system	1371:1396	a conventional HPLC system	1371:1396	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	2	25	theme	final	468:472	arg1	ingredient					496:505	final active pharmaceutical ingredient	468:505	final active pharmaceutical ingredient (API)	468:511	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	25	theme	final	468:472	arg1	API					508:510	API	508:510	API	508:510	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	1	26	theme	precursor	195:203	arg1	protein					205:211	β-site amyloid precursor protein	180:211	β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease	180:313	Verubecestat is an inhibitor of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) being evaluated in clinical trials for the treatment of Alzheimer's disease.
29397980	0	27	theme	glycopeptide	12:23	arg1	selectors					32:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	6	28	theme	technical	1411:1419	arg1	preparation					1429:1439	technical package preparation	1411:1439	technical package preparation	1411:1439	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	2	29	theme	route	326:330	arg1	development					332:342	Synthetic route development	316:342	Synthetic route development	316:342	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	30	theme	excess	417:422	arg1	determination					429:441	enantiomeric excess (ee) determination	404:441	enantiomeric excess (ee) determination of each intermediate	404:462	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	0	31	theme	Macrocyclic	0:10	arg1	selectors					32:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	4	32	theme	triethylammonium	899:914	arg1	acetate					916:922	triethylammonium acetate	899:922	triethylammonium acetate	899:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	2	33	theme	Synthetic	316:324	arg1	route					326:330	Synthetic route	316:330	Synthetic route development	316:342	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	34	theme	enantiomeric	404:415	arg1	ee					425:426	ee	425:426	ee	425:426	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	34	theme	enantiomeric	404:415	arg1	excess					417:422	enantiomeric excess	404:422	enantiomeric excess (ee) determination of each intermediate	404:462	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	4	35	theme	good	992:995	arg1	enatioseparation					997:1012	good enatioseparation	992:1012	good enatioseparation of all verubecestat intermediates	992:1046	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	5	36	theme	macrocyclic	1124:1134	arg1	selector					1156:1163	another macrocyclic glycopeptide chiral selector	1116:1163	another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles	1116:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	5	36	theme	macrocyclic	1124:1134	arg1	NicoShell					1105:1113	NicoShell	1105:1113	NicoShell	1105:1113	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	4	37	theme	chiral	804:809	arg1	Teicoplanin					821:831	Teicoplanin	821:831	Teicoplanin	821:831	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	37	theme	chiral	804:809	arg1	selector					811:818	a single chiral selector	795:818	a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate	795:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	2	38	theme	intermediate	451:462	arg1	determination					429:441	enantiomeric excess (ee) determination	404:441	enantiomeric excess (ee) determination of each intermediate	404:462	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	38	theme	intermediate	451:462	arg1	API					508:510	API	508:510	API	508:510	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	38	theme	intermediate	451:462	arg1	ingredient					496:505	final active pharmaceutical ingredient	468:505	final active pharmaceutical ingredient (API)	468:511	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	0	39	theme	route	141:145	arg1	analysis					95:102	enantiopurity analysis	81:102	enantiopurity analysis of the entire verubecestat synthetic route	81:145	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	6	40	theme	route	1314:1318	arg1	analysis					1268:1275	fast and reliable enantiopurity analysis	1236:1275	fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns	1236:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	6	40	theme	route	1314:1318	arg1	phases					1361:1366	mobile phases	1354:1366	mobile phases	1354:1366	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	4	41	theme	single	797:802	arg1	Teicoplanin					821:831	Teicoplanin	821:831	Teicoplanin	821:831	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	41	theme	single	797:802	arg1	selector					811:818	a single chiral selector	795:818	a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate	795:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	6	42	theme	method	1442:1447	arg1	validation					1449:1458	method validation	1442:1458	method validation	1442:1458	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	43	theme	analytical	518:527	arg1	package					539:545	The analytical technical package	514:545	The analytical technical package of validated methods	514:566	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	5	44	theme	glycopeptide	1136:1147	arg1	selector					1156:1163	another macrocyclic glycopeptide chiral selector	1116:1163	another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles	1116:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	5	44	theme	glycopeptide	1136:1147	arg1	NicoShell					1105:1113	NicoShell	1105:1113	NicoShell	1105:1113	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	3	45	dep	polysaccharide-based	653:672	arg1	CSPs					700:703	CSPs	700:703	CSPs	700:703	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	45	dep	polysaccharide-based	653:672	arg1	phases					692:697	chiral stationary phases	674:697	chiral stationary phases (CSPs)	674:704	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	45	dep	polysaccharide-based	653:672	arg1	phases					717:722	phases	717:722	phases	717:722	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	46	theme	stationary	681:690	arg1	CSPs					700:703	CSPs	700:703	CSPs	700:703	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	46	theme	stationary	681:690	arg1	phases					692:697	chiral stationary phases	674:697	chiral stationary phases (CSPs)	674:704	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	0	47	gly	glycopeptide	12:23	arg2	glycopeptide					12:23	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	4	48	theme	intermediates	1034:1046	arg1	enatioseparation					997:1012	good enatioseparation	992:1012	good enatioseparation of all verubecestat intermediates	992:1046	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	49	theme	verubecestat	1021:1032	arg1	intermediates					1034:1046	all verubecestat intermediates	1017:1046	all verubecestat intermediates	1017:1046	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	50	theme	different	951:959	arg1	compositions					971:982	different isocratic compositions	951:982	different isocratic compositions	951:982	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	5	51	theme	porous	1196:1201	arg1	particles					1203:1211	2.7 μm superficially porous particles	1175:1211	2.7 μm superficially porous particles	1175:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	4	52	theme	developed	761:769	arg1	columns					778:784	recently developed chiral columns	752:784	recently developed chiral columns	752:784	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	0	53	theme	core-shell	52:61	arg1	particles					63:71	core-shell particles	52:71	core-shell particles	52:71	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	6	54	theme	synthetic	1304:1312	arg1	route					1314:1318	the entire verubecestat synthetic route	1280:1318	the entire verubecestat synthetic route using only two chiral columns	1280:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	2	55	theme	pharmaceutical	481:494	arg1	ingredient					496:505	final active pharmaceutical ingredient	468:505	final active pharmaceutical ingredient (API)	468:511	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	55	theme	pharmaceutical	481:494	arg1	API					508:510	API	508:510	API	508:510	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	3	56	theme	multiple	626:633	arg1	combinations					724:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	2	57	theme	diastereoselective	353:370	arg1	transformations					372:386	diastereoselective transformations	353:386	diastereoselective transformations	353:386	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	4	58	theme	2.7 μm	844:849	arg1	particles					862:870	2.7 μm core-shell particles	844:870	2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate	844:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	5	59	theme	chiral	1149:1154	arg1	selector					1156:1163	another macrocyclic glycopeptide chiral selector	1116:1163	another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles	1116:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	5	59	theme	chiral	1149:1154	arg1	NicoShell					1105:1113	NicoShell	1105:1113	NicoShell	1105:1113	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	3	60	theme	polysaccharide-based	653:672	arg1	combinations					724:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	6	61	theme	mobile	1354:1359	arg1	phases					1361:1366	mobile phases	1354:1366	mobile phases	1354:1366	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	4	62	theme	columns	778:784	arg1	Evaluation					738:747	Evaluation	738:747	Evaluation of recently developed chiral columns	738:784	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	63	theme	isocratic	961:969	arg1	compositions					971:982	different isocratic compositions	951:982	different isocratic compositions	951:982	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	6	64	theme	chiral	1335:1340	arg1	columns					1342:1348	only two chiral columns	1326:1348	only two chiral columns	1326:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	65	theme	chiral	674:679	arg1	CSPs					700:703	CSPs	700:703	CSPs	700:703	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	65	theme	chiral	674:679	arg1	phases					692:697	chiral stationary phases	674:697	chiral stationary phases (CSPs)	674:704	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	3	66	theme	validated	550:558	arg1	methods					560:566	validated methods	550:566	validated methods	550:566	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	6	67	theme	fast	1236:1239	arg1	analysis					1268:1275	fast and reliable enantiopurity analysis	1236:1275	fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns	1236:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	0	68	theme	enantiopurity	81:93	arg1	analysis					95:102	enantiopurity analysis	81:102	enantiopurity analysis of the entire verubecestat synthetic route	81:145	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	5	69	theme	bonded	1165:1170	arg1	selector					1156:1163	another macrocyclic glycopeptide chiral selector	1116:1163	another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles	1116:1211	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	5	69	theme	bonded	1165:1170	arg1	NicoShell					1105:1113	NicoShell	1105:1113	NicoShell	1105:1113	EE determination of verubecestat is easily performed on NicoShell, another macrocyclic glycopeptide chiral selector bonded to 2.7 μm superficially porous particles.
29397980	4	70	theme	core-shell	851:860	arg1	particles					862:870	2.7 μm core-shell particles	844:870	2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate	844:922	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	4	71	from	compositions	971:982	arg1	eluents					940:946	methanol based eluents	925:946	methanol based eluents at different isocratic compositions	925:982	Evaluation of recently developed chiral columns revealed a single chiral selector (Teicoplanin) bonded to 2.7 μm core-shell particles using H3PO4 in H2O/ACN and triethylammonium acetate: methanol based eluents at different isocratic compositions allowed good enatioseparation of all verubecestat intermediates.
29397980	6	72	theme	verubecestat	1291:1302	arg1	route					1314:1318	the entire verubecestat synthetic route	1280:1318	the entire verubecestat synthetic route using only two chiral columns	1280:1348	This approach enables fast and reliable enantiopurity analysis of the entire verubecestat synthetic route using only two chiral columns and mobile phases on a conventional HPLC system, simplifying technical package preparation, method validation and transfer to manufacturing facilities.
29397980	3	73	theme	mobile	710:715	arg1	combinations					724:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations	626:735	The analytical technical package of validated methods relies on enantioselective SFC and RPLC separations using multiple 3 and 5 μm coated polysaccharide-based chiral stationary phases (CSPs) and mobile phases combinations.
29397980	0	74	theme	chiral	25:30	arg1	selectors					32:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors	0:40	Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.
29397980	2	75	theme	active	474:479	arg1	ingredient					496:505	final active pharmaceutical ingredient	468:505	final active pharmaceutical ingredient (API)	468:511	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
29397980	2	75	theme	active	474:479	arg1	API					508:510	API	508:510	API	508:510	Synthetic route development involves diastereoselective transformations with a need for enantiomeric excess (ee) determination of each intermediate and final active pharmaceutical ingredient (API).
31457009	5	0	theme	cisternae	763:771	arg1	number					753:758	the number	749:758	the number of cisternae	749:771	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	6	1	theme	functional	983:992	arg1	modulation					1024:1033	the modulation	1020:1033	the modulation of glycan-mediated physiological functions	1020:1076	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	6	1	theme	functional	983:992	arg1	investigation					994:1006	the functional investigation	979:1006	the functional investigation of glycans	979:1017	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	3	2	theme	glycosylation	345:357	arg1	machinery					359:367	the glycosylation machinery	341:367	the glycosylation machinery	341:367	This heterogeneity is not random but is controlled by the organization of the glycosylation machinery in the Golgi cisternae.
31457009	5	3	theme	N-glycosylation	662:676	arg1	potential					678:686	the N-glycosylation potential	658:686	the N-glycosylation potential	658:686	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	2	4	from	structures	192:201	arg1	molecules					215:223	separate molecules	206:223	separate molecules of the same protein	206:243	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	4	5	theme	process	459:465	arg1	model					430:434	a computational model	414:434	a computational model of the N-glycosylation process	414:465	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	4	6	theme	differing	589:597	arg1	degrees					599:605	differing degrees	589:605	differing degrees of heterogeneity	589:622	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	2	7	from	molecules	215:223	arg1	variety					174:180	a variety	172:180	a variety of glycan structures on separate molecules of the same protein, known as glycoforms	172:264	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	2	7	from	molecules	215:223	arg1	structures					192:201	glycan structures	185:201	glycan structures	185:201	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	4	8	theme	N-glycosylation	443:457	arg1	process					459:465	the N-glycosylation process	439:465	the N-glycosylation process	439:465	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	5	9	theme	near	802:805	arg1	homogeneity					807:817	near homogeneity	802:817	near homogeneity for N-glycans	802:831	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	6	10	theme	computational	887:899	arg1	study					901:905	this computational study	882:905	this computational study	882:905	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	0	11	theme	N-Glycosylation	4:18	arg1	Potential					31:39	The N-Glycosylation Processing Potential	0:39	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus	0:72	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus.
31457009	2	12	theme	Mammalian	142:150	arg1	cells					152:156	Mammalian cells	142:156	Mammalian cells	142:156	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	6	13	theme	functions	1068:1076	arg1	modulation					1024:1033	the modulation	1020:1033	the modulation of glycan-mediated physiological functions	1020:1076	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	6	13	theme	functions	1068:1076	arg1	investigation					994:1006	the functional investigation	979:1006	the functional investigation of glycans	979:1017	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	2	14	theme	structures	192:201	arg1	variety					174:180	a variety	172:180	a variety of glycan structures on separate molecules of the same protein, known as glycoforms	172:264	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	2	14	theme	structures	192:201	arg1	structures					192:201	glycan structures	185:201	glycan structures	185:201	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	1	15	theme	inherent	95:102	arg1	Heterogeneity					75:87	Heterogeneity	75:87	Heterogeneity	75:87	Heterogeneity is an inherent feature of the glycosylation process.
31457009	1	15	theme	inherent	95:102	arg1	feature					104:110	an inherent feature	92:110	an inherent feature of the glycosylation process	92:139	Heterogeneity is an inherent feature of the glycosylation process.
31457009	6	16	theme	physiological	1054:1066	arg1	functions					1068:1076	glycan-mediated physiological functions	1038:1076	glycan-mediated physiological functions	1038:1076	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	5	17	dep	demonstrate	646:656	arg1	limits					773:778	limits	773:778	limits the goal of achieving near homogeneity for N-glycans	773:831	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	4	18	theme	different	533:541	arg1	cisternae					543:551	different cisternae	533:551	different cisternae	533:551	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	5	19	theme	mammalian	706:714	arg1	apparatus					722:730	the mammalian Golgi apparatus	702:730	the mammalian Golgi apparatus	702:730	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	6	20	theme	glycan-mediated	1038:1052	arg1	functions					1068:1076	glycan-mediated physiological functions	1038:1076	glycan-mediated physiological functions	1038:1076	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	0	21	theme	Processing	20:29	arg1	Potential					31:39	The N-Glycosylation Processing Potential	0:39	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus	0:72	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus.
31457009	4	22	theme	N-glycan	560:567	arg1	biosynthesis					569:580	N-glycan biosynthesis	560:580	N-glycan biosynthesis	560:580	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	5	23	theme	Golgi	716:720	arg1	apparatus					722:730	the mammalian Golgi apparatus	702:730	the mammalian Golgi apparatus	702:730	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	2	24	theme	glycan	185:190	arg1	structures					192:201	glycan structures	185:201	glycan structures	185:201	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	5	25	theme	apparatus	722:730	arg1	limits					692:697	limits	692:697	limits	692:697	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	5	25	theme	apparatus	722:730	arg1	potential					678:686	the N-glycosylation potential	658:686	the N-glycosylation potential	658:686	Using this model, we demonstrate the N-glycosylation potential and limits of the mammalian Golgi apparatus, for example how the number of cisternae limits the goal of achieving near homogeneity for N-glycans.
31457009	4	26	theme	computational	416:428	arg1	model					430:434	a computational model	414:434	a computational model of the N-glycosylation process	414:465	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	2	27	theme	protein	237:243	arg1	molecules					215:223	separate molecules	206:223	separate molecules of the same protein	206:243	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	4	28	theme	glycosylation	504:516	arg1	machinery					518:526	the glycosylation machinery	500:526	the glycosylation machinery	500:526	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	6	29	theme	glycoforms	861:870	arg1	production					838:847	The production	834:847	The production of specific glycoforms guided by this computational study	834:905	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	2	30	theme	same	232:235	arg1	protein					237:243	the same protein	228:243	the same protein	228:243	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	0	31	theme	Golgi	58:62	arg1	Apparatus					64:72	the Mammalian Golgi Apparatus	44:72	the Mammalian Golgi Apparatus	44:72	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus.
31457009	6	32	theme	specific	852:859	arg1	glycoforms					861:870	specific glycoforms	852:870	specific glycoforms guided by this computational study	852:905	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	0	33	theme	Mammalian	48:56	arg1	Apparatus					64:72	the Mammalian Golgi Apparatus	44:72	the Mammalian Golgi Apparatus	44:72	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus.
31457009	6	34	theme	glycoform	931:939	arg1	engineering					941:951	"glycoform engineering	930:951	"glycoform engineering	930:951	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	6	34	theme	glycoform	931:939	arg1	"					953:953	"	953:953	" which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology	953:1098	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	1	35	theme	glycosylation	119:131	arg1	process					133:139	the glycosylation process	115:139	the glycosylation process	115:139	Heterogeneity is an inherent feature of the glycosylation process.
31457009	4	36	theme	machinery	518:526	arg1	organization					484:495	the organization	480:495	the organization of the glycosylation machinery into different cisternae	480:551	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	2	37	theme	separate	206:213	arg1	molecules					215:223	separate molecules	206:223	separate molecules of the same protein	206:243	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	1	38	theme	process	133:139	arg1	Heterogeneity					75:87	Heterogeneity	75:87	Heterogeneity	75:87	Heterogeneity is an inherent feature of the glycosylation process.
31457009	1	38	theme	process	133:139	arg1	feature					104:110	an inherent feature	92:110	an inherent feature of the glycosylation process	92:139	Heterogeneity is an inherent feature of the glycosylation process.
31457009	4	39	theme	heterogeneity	610:622	arg1	degrees					599:605	differing degrees	589:605	differing degrees of heterogeneity	589:622	In this work, we use a computational model of the N-glycosylation process to probe how the organization of the glycosylation machinery into different cisternae drives N-glycan biosynthesis toward differing degrees of heterogeneity.
31457009	6	40	theme	glycans	1011:1017	arg1	modulation					1024:1033	the modulation	1020:1033	the modulation of glycan-mediated physiological functions	1020:1076	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	6	40	theme	glycans	1011:1017	arg1	investigation					994:1006	the functional investigation	979:1006	the functional investigation of glycans	979:1017	The production of specific glycoforms guided by this computational study could pave the way for "glycoform engineering," which will find uses in the functional investigation of glycans, the modulation of glycan-mediated physiological functions, and in biotechnology.
31457009	2	41	from	variety	174:180	arg1	molecules					215:223	separate molecules	206:223	separate molecules of the same protein	206:243	Mammalian cells often produce a variety of glycan structures on separate molecules of the same protein, known as glycoforms.
31457009	3	42	from	organization	325:336	arg1	cisternae					382:390	the Golgi cisternae	372:390	the Golgi cisternae	372:390	This heterogeneity is not random but is controlled by the organization of the glycosylation machinery in the Golgi cisternae.
31457009	0	43	theme	Apparatus	64:72	arg1	Potential					31:39	The N-Glycosylation Processing Potential	0:39	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus	0:72	The N-Glycosylation Processing Potential of the Mammalian Golgi Apparatus.
31457009	3	44	theme	Golgi	376:380	arg1	cisternae					382:390	the Golgi cisternae	372:390	the Golgi cisternae	372:390	This heterogeneity is not random but is controlled by the organization of the glycosylation machinery in the Golgi cisternae.
31457009	3	45	theme	machinery	359:367	arg1	organization					325:336	the organization	321:336	the organization of the glycosylation machinery in the Golgi cisternae	321:390	This heterogeneity is not random but is controlled by the organization of the glycosylation machinery in the Golgi cisternae.
30445090	0	0	from	profiles	21:28	arg1	glabra					90:95	glabra	90:95	glabra	90:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	Gal					863:865	Gal	863:865	Gal	863:865	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	Rha					868:870	Rha	868:870	Rha	868:870	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	Glc					888:890	Glc	888:890	Glc	888:890	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	monosaccharides					846:860	The five monosaccharides	837:860	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc)	837:891	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	Xyl					873:875	Xyl	873:875	Xyl	873:875	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	1	dep	monosaccharides	846:860	arg1	GlcA					878:881	GlcA	878:881	GlcA	878:881	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	2	2	used	used	444:447	arg2	Chemometrics					427:438	Chemometrics	427:438	Chemometrics	427:438	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	1	3	theme	Fourier-transform	184:200	arg1	FT-IR					225:229	FT-IR	225:229	FT-IR	225:229	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	3	theme	Fourier-transform	184:200	arg1	spectroscopy					211:222	Fourier-transform infrared spectroscopy	184:222	Fourier-transform infrared spectroscopy (FT-IR)	184:230	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	3	4	theme	FT-IR	715:719	arg1	fingerprints					721:732	the HPSEC, PCD-HPLC, and FT-IR fingerprints	690:732	fingerprints	721:732	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	4	5	theme	clustering	793:802	arg1	analysis					804:811	clustering analysis	793:811	clustering analysis based on their nuances	793:834	The samples from different regions could be classified by clustering analysis based on their nuances.
30445090	3	6	contain	had	649:651	arg2	degree					660:665	a high degree	653:665	a high degree of similarity	653:679	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	3	6	contain	had	649:651	arg1	polysaccharides					597:611	polysaccharides	597:611	polysaccharides from 18 batches of Sarcandra glabra	597:647	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	5	7	dep	FT-IR	916:920	arg1	1411 cm-1					937:945	1411 cm-1	937:945	1411 cm-1	937:945	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	7	dep	FT-IR	916:920	arg1	3371 cm-1					923:931	3371 cm-1	923:931	3371 cm-1	923:931	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	3	8	theme	present	571:577	arg1	study					579:583	the present study	567:583	the present study	567:583	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	1	9	theme	infrared	202:209	arg1	FT-IR					225:229	FT-IR	225:229	FT-IR	225:229	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	9	theme	infrared	202:209	arg1	spectroscopy					211:222	Fourier-transform infrared spectroscopy	184:222	Fourier-transform infrared spectroscopy (FT-IR)	184:230	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	0	10	from	analysis	47:54	arg1	glabra					90:95	glabra	90:95	glabra	90:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	1	11	from	analysis	344:351	arg1	SGPs					399:402	SGPs	399:402	SGPs	399:402	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	11	from	analysis	344:351	arg1	glabra					391:396	glabra	391:396	glabra	391:396	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	3	12	theme	study	579:583	arg1	results					556:562	The results	552:562	The results of the present study	552:583	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	0	13	theme	fingerprint	9:19	arg1	profiles					21:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	2	14	from	regions	515:521	arg1	SGPs					495:498	SGPs	495:498	SGPs from different regions based on their fingerprints	495:549	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	2	14	from	regions	515:521	arg1	differences					480:490	differences	480:490	differences	480:490	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	2	14	from	regions	515:521	arg1	similarity					465:474	similarity	465:474	similarity	465:474	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	1	15	theme	Multiple	98:105	arg1	chromatography					160:173	high performance size-exclusion chromatography	128:173	high performance size-exclusion chromatography (HPSEC)	128:181	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	15	theme	Multiple	98:105	arg1	techniques					107:116	Multiple techniques	98:116	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	98:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	15	theme	Multiple	98:105	arg1	spectroscopy					211:222	Fourier-transform infrared spectroscopy	184:222	Fourier-transform infrared spectroscopy (FT-IR)	184:230	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	15	theme	Multiple	98:105	arg1	chromatography					286:299	pre-column derivatization high-performance liquid chromatography	236:299	pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	236:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	0	16	theme	Multiple	0:7	arg1	profiles					21:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	4	17	from	regions	762:768	arg1	samples					739:745	The samples	735:745	The samples from different regions	735:768	The samples from different regions could be classified by clustering analysis based on their nuances.
30445090	1	18	theme	fingerprint	332:342	arg1	analysis					344:351	the fingerprint analysis	328:351	the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions	328:424	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	19	theme	high	128:131	arg1	chromatography					160:173	high performance size-exclusion chromatography	128:173	high performance size-exclusion chromatography (HPSEC)	128:181	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	19	theme	high	128:131	arg1	HPSEC					176:180	HPSEC	176:180	HPSEC	176:180	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	0	20	from	glabra	90:95	arg1	profiles					21:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	0	20	from	glabra	90:95	arg1	analysis					47:54	chemometrics analysis	34:54	chemometrics analysis of polysaccharides from Sarcandra glabra	34:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	0	20	from	glabra	90:95	arg1	polysaccharides					59:73	polysaccharides	59:73	polysaccharides from Sarcandra glabra	59:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	3	21	theme	glabra	642:647	arg1	batches					621:627	18 batches	618:627	18 batches of Sarcandra glabra	618:647	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	1	22	theme	pre-column	236:245	arg1	chromatography					286:299	pre-column derivatization high-performance liquid chromatography	236:299	pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	236:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	22	theme	pre-column	236:245	arg1	PCD-HPLC					302:309	PCD-HPLC	302:309	PCD-HPLC	302:309	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	0	23	theme	chemometrics	34:45	arg1	analysis					47:54	chemometrics analysis	34:54	chemometrics analysis of polysaccharides from Sarcandra glabra	34:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	3	24	theme	similarity	670:679	arg1	degree					660:665	a high degree	653:665	a high degree of similarity	653:679	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	5	25	theme	quality	990:996	arg1	control					998:1004	the quality control	986:1004	the quality control of Sarcandra glabra	986:1024	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	1	26	theme	derivatization	247:260	arg1	chromatography					286:299	pre-column derivatization high-performance liquid chromatography	236:299	pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	236:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	26	theme	derivatization	247:260	arg1	PCD-HPLC					302:309	PCD-HPLC	302:309	PCD-HPLC	302:309	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	0	27	theme	polysaccharides	59:73	arg1	profiles					21:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles	0:28	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	0	27	theme	polysaccharides	59:73	arg1	analysis					47:54	chemometrics analysis	34:54	chemometrics analysis of polysaccharides from Sarcandra glabra	34:95	Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.
30445090	2	28	dep	similarity	465:474	arg1	the					461:463	the	461:463	the	461:463	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	1	29	theme	different	408:416	arg1	regions					418:424	different regions	408:424	different regions	408:424	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	30	theme	performance	133:143	arg1	chromatography					160:173	high performance size-exclusion chromatography	128:173	high performance size-exclusion chromatography (HPSEC)	128:181	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	30	theme	performance	133:143	arg1	HPSEC					176:180	HPSEC	176:180	HPSEC	176:180	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	31	from	glabra	391:396	arg1	analysis					344:351	the fingerprint analysis	328:351	the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions	328:424	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	31	from	glabra	391:396	arg1	polysaccharides					360:374	the polysaccharides	356:374	the polysaccharides from Sarcandra glabra (SGPs) in different regions	356:424	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	32	theme	polysaccharides	360:374	arg1	analysis					344:351	the fingerprint analysis	328:351	the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions	328:424	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	3	33	theme	Sarcandra	632:640	arg1	glabra					642:647	Sarcandra glabra	632:647	Sarcandra glabra	632:647	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	1	34	theme	size-exclusion	145:158	arg1	chromatography					160:173	high performance size-exclusion chromatography	128:173	high performance size-exclusion chromatography (HPSEC)	128:181	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	34	theme	size-exclusion	145:158	arg1	HPSEC					176:180	HPSEC	176:180	HPSEC	176:180	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	5	35	theme	glabra	1019:1024	arg1	control					998:1004	the quality control	986:1004	the quality control of Sarcandra glabra	986:1024	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	4	36	theme	different	752:760	arg1	regions					762:768	different regions	752:768	different regions	752:768	The samples from different regions could be classified by clustering analysis based on their nuances.
30445090	1	37	theme	high-performance	262:277	arg1	chromatography					286:299	pre-column derivatization high-performance liquid chromatography	236:299	pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	236:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	37	theme	high-performance	262:277	arg1	PCD-HPLC					302:309	PCD-HPLC	302:309	PCD-HPLC	302:309	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	3	38	theme	HPSEC	694:698	arg1	PCD-HPLC					701:708	the HPSEC, PCD-HPLC, and FT-IR fingerprints	690:732	PCD-HPLC	701:708	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	5	39	theme	herb	969:972	arg1	markers					974:980	herb markers	969:980	herb markers for the quality control of Sarcandra glabra	969:1024	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	39	theme	herb	969:972	arg1	wavelengths					901:911	the wavelengths	897:911	the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1)	897:946	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	39	theme	herb	969:972	arg1	monosaccharides					846:860	The five monosaccharides	837:860	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc)	837:891	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	markers					974:980	herb markers	969:980	herb markers for the quality control of Sarcandra glabra	969:1024	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	wavelengths					901:911	the wavelengths	897:911	the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1)	897:946	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	Gal					863:865	Gal	863:865	Gal	863:865	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	Rha					868:870	Rha	868:870	Rha	868:870	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	Glc					888:890	Glc	888:890	Glc	888:890	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	monosaccharides					846:860	The five monosaccharides	837:860	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc)	837:891	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	Xyl					873:875	Xyl	873:875	Xyl	873:875	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	5	40	theme	FT-IR	916:920	arg1	GlcA					878:881	GlcA	878:881	GlcA	878:881	The five monosaccharides (Gal, Rha, Xyl, GlcA, and Glc) and the wavelengths of FT-IR (3371 cm-1 and 1411 cm-1) could be selected as herb markers for the quality control of Sarcandra glabra.
30445090	1	41	theme	liquid	279:284	arg1	chromatography					286:299	pre-column derivatization high-performance liquid chromatography	236:299	pre-column derivatization high-performance liquid chromatography (PCD-HPLC)	236:310	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	1	41	theme	liquid	279:284	arg1	PCD-HPLC					302:309	PCD-HPLC	302:309	PCD-HPLC	302:309	Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different regions.
30445090	3	42	from	batches	621:627	arg1	polysaccharides					597:611	polysaccharides	597:611	polysaccharides from 18 batches of Sarcandra glabra	597:647	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	2	43	theme	different	505:513	arg1	regions					515:521	different regions	505:521	different regions based on their fingerprints	505:549	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	2	44	from	differences	480:490	arg1	regions					515:521	different regions	505:521	different regions based on their fingerprints	505:549	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	3	45	theme	high	655:658	arg1	degree					660:665	a high degree	653:665	a high degree of similarity	653:679	The results of the present study showed that polysaccharides from 18 batches of Sarcandra glabra had a high degree of similarity based on the HPSEC, PCD-HPLC, and FT-IR fingerprints.
30445090	2	46	from	similarity	465:474	arg1	regions					515:521	different regions	505:521	different regions based on their fingerprints	505:549	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	2	47	theme	SGPs	495:498	arg1	differences					480:490	differences	480:490	differences	480:490	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30445090	2	47	theme	SGPs	495:498	arg1	similarity					465:474	similarity	465:474	similarity	465:474	Chemometrics was used to evaluate the similarity and differences of SGPs from different regions based on their fingerprints.
30641994	1	0	theme	diseases	330:337	arg1	treatment					281:289	the treatment	277:289	the treatment of neurasthenia, tumors, and digestive diseases	277:337	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	7	1	theme	strain	1479:1484	arg1	content					1442:1448	high polysaccharide content breeding	1422:1457	high polysaccharide content breeding of the H. erinaceus strain	1422:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	2	2	theme	polysaccharide	358:371	arg1	components					373:382	Six intracellular polysaccharide components	340:382	Six intracellular polysaccharide components	340:382	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	3	dep	strain	503:508	arg1	atmospheric					452:462	atmospheric	452:462	atmospheric	452:462	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	3	dep	strain	503:508	arg1	temperature					473:483	room temperature	468:483	room temperature	468:483	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	6	4	theme	ethanol	1263:1269	arg1	stimulation					1244:1254	stimulation	1244:1254	stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis	1244:1344	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	0	5	theme	Mutagenesis	58:68	arg1	Effects					0:6	Effects	0:6	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.	0:171	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	6	6	theme	H.	1305:1306	arg1	erinaceus					1308:1316	H. erinaceus	1305:1316	H. erinaceus induced by ARTP mutagenesis	1305:1344	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	2	7	theme	intracellular	344:356	arg1	components					373:382	Six intracellular polysaccharide components	340:382	Six intracellular polysaccharide components	340:382	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	7	8	theme	product	1565:1571	arg1	development					1573:1583	healthy product development	1557:1583	healthy product development	1557:1583	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	2	9	theme	original	524:531	arg1	0605					541:544	0605	541:544	0605	541:544	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	9	theme	original	524:531	arg1	strain					533:538	the original strain	520:538	the original strain (0605)	520:545	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	0	10	from	Effects	0:6	arg1	Characteristics					89:103	Physicochemical Characteristics	73:103	Physicochemical Characteristics	73:103	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	0	10	from	Effects	0:6	arg1	Activity					116:123	Immune Activity	109:123	Immune Activity In Vitro	109:132	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	4	11	theme	body	791:794	arg1	yield					773:777	the yield	769:777	the yield of fruiting body cultivated by mutated strain increased by 22%	769:840	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	4	11	theme	body	791:794	arg1	content					865:871	the polysaccharide content	846:871	the polysaccharide content	846:871	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	5	12	theme	polysaccharide	1020:1033	arg1	components					1035:1044	polysaccharide components	1020:1044	polysaccharide components from H. erinaceus induced by ARTP mutagenesis	1020:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	0	13	theme	Physicochemical	73:87	arg1	Characteristics					89:103	Physicochemical Characteristics	73:103	Physicochemical Characteristics	73:103	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	6	14	theme	TNF-α	1184:1188	arg1	production					1190:1199	TNF-α production	1184:1199	TNF-α production	1184:1199	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	6	15	theme	macrophages	1215:1225	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	4	16	theme	fruiting	782:789	arg1	body					791:794	fruiting body	782:794	fruiting body cultivated by mutated strain increased by 22%	782:840	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	7	17	theme	polysaccharide	1427:1440	arg1	content					1442:1448	high polysaccharide content breeding	1422:1457	high polysaccharide content breeding of the H. erinaceus strain	1422:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	0	18	theme	Immune	109:114	arg1	Activity					116:123	Immune Activity	109:123	Immune Activity In Vitro	109:132	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	5	19	theme	original	1138:1145	arg1	one					1147:1149	the original one	1134:1149	the original one	1134:1149	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	6	20	theme	ARTP	1329:1332	arg1	mutagenesis					1334:1344	ARTP mutagenesis	1329:1344	ARTP mutagenesis	1329:1344	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	5	21	from	compositions	1004:1015	arg1	erinaceus					1054:1062	H. erinaceus	1051:1062	H. erinaceus induced by ARTP mutagenesis	1051:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	22	theme	monosaccharide	989:1002	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	22	theme	monosaccharide	989:1002	arg1	compositions					1004:1015	the monosaccharide compositions	985:1015	the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis	985:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	2	23	theme	erinaceus	406:414	arg1	bodies					425:430	H. erinaceus fruiting bodies	403:430	H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605)	403:545	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	24	theme	room	468:471	arg1	temperature					473:483	room temperature	468:483	room temperature	468:483	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	7	25	theme	erinaceus	1469:1477	arg1	strain					1479:1484	the H. erinaceus strain	1462:1484	the H. erinaceus strain	1462:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	3	26	from	activities	709:718	arg1	macrophages					732:742	RAW264.7 macrophages	723:742	RAW264.7 macrophages	723:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	6	27	theme	IL-10	1166:1170	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	2	28	theme	H.	403:404	arg1	bodies					425:430	H. erinaceus fruiting bodies	403:430	H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605)	403:545	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	29	theme	mutagenic	493:501	arg1	321					511:513	321	511:513	321	511:513	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	29	theme	mutagenic	493:501	arg1	strain					503:508	ARTP (atmospheric and room temperature plasma) mutagenic strain	446:508	ARTP (atmospheric and room temperature plasma) mutagenic strain (321)	446:514	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	6	30	theme	%	1261:1261	arg1	ethanol					1263:1269	20% ethanol	1259:1269	20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis	1259:1344	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	6	31	theme	IL-1β	1173:1177	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	3	32	from	characteristics	621:635	arg1	macrophages					732:742	RAW264.7 macrophages	723:742	RAW264.7 macrophages	723:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	7	33	theme	quality	1535:1541	arg1	resources					1543:1551	high quality resources	1530:1551	high quality resources	1530:1551	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	2	34	dep	atmospheric	452:462	arg1	plasma					485:490	plasma	485:490	plasma	485:490	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	5	35	theme	ARTP	1075:1078	arg1	mutagenesis					1080:1090	ARTP mutagenesis	1075:1090	ARTP mutagenesis	1075:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	3	36	theme	components	661:670	arg1	activities					709:718	their potential immunomodulatory activities	676:718	their potential immunomodulatory activities on RAW264.7 macrophages	676:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	3	36	theme	components	661:670	arg1	characteristics					621:635	the physicochemical characteristics	601:635	the physicochemical characteristics of these polysaccharide components	601:670	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	4	37	theme	ARTP	929:932	arg1	mutagenesis					934:944	ARTP mutagenesis	929:944	ARTP mutagenesis	929:944	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	7	38	theme	H.	1590:1591	arg1	erinaceus					1593:1601	H. erinaceus	1590:1601	H. erinaceus	1590:1601	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	5	39	theme	weight	961:966	arg1	distribution					968:979	The molecular weight distribution	947:979	The molecular weight distribution	947:979	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	39	theme	weight	961:966	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	7	40	theme	practical	1401:1409	arg1	ARTP					1376:1379	ARTP	1376:1379	ARTP	1376:1379	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	7	40	theme	practical	1401:1409	arg1	method					1411:1416	an efficient and practical method	1384:1416	an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain	1384:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	1	41	theme	main	199:202	arg1	substance					211:219	the main active substance	195:219	the main active substance contained in Hericium erinaceus	195:251	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	1	41	theme	main	199:202	arg1	polysaccharide					177:190	The polysaccharide	173:190	The polysaccharide	173:190	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	0	42	theme	Atmospheric	11:21	arg1	Mutagenesis					58:68	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	11:68	Mutagenesis	58:68	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	6	43	theme	production	1190:1199	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	5	44	theme	molecular	951:959	arg1	distribution					968:979	The molecular weight distribution	947:979	The molecular weight distribution	947:979	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	44	theme	molecular	951:959	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	3	45	theme	polysaccharide	646:659	arg1	components					661:670	these polysaccharide components	640:670	these polysaccharide components	640:670	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	7	46	theme	high	1422:1425	arg1	content					1442:1448	high polysaccharide content breeding	1422:1457	high polysaccharide content breeding of the H. erinaceus strain	1422:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	1	47	theme	active	204:209	arg1	substance					211:219	the main active substance	195:219	the main active substance contained in Hericium erinaceus	195:251	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	1	47	theme	active	204:209	arg1	polysaccharide					177:190	The polysaccharide	173:190	The polysaccharide	173:190	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	0	48	theme	Temperature	32:42	arg1	Mutagenesis					58:68	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	11:68	Mutagenesis	58:68	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	1	49	theme	neurasthenia	294:305	arg1	treatment					281:289	the treatment	277:289	the treatment of neurasthenia, tumors, and digestive diseases	277:337	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	1	50	used	used	269:272	arg2	substance					211:219	the main active substance	195:219	the main active substance contained in Hericium erinaceus	195:251	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	1	50	used	used	269:272	arg2	polysaccharide					177:190	The polysaccharide	173:190	The polysaccharide	173:190	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	5	51	theme	H.	1051:1052	arg1	erinaceus					1054:1062	H. erinaceus	1051:1062	H. erinaceus induced by ARTP mutagenesis	1051:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	52	from	erinaceus	1054:1062	arg1	components					1035:1044	polysaccharide components	1020:1044	polysaccharide components from H. erinaceus induced by ARTP mutagenesis	1020:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	52	from	erinaceus	1054:1062	arg1	distribution					968:979	The molecular weight distribution	947:979	The molecular weight distribution	947:979	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	52	from	erinaceus	1054:1062	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	52	from	erinaceus	1054:1062	arg1	compositions					1004:1015	the monosaccharide compositions	985:1015	the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis	985:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	7	53	theme	H.	1466:1467	arg1	strain					1479:1484	the H. erinaceus strain	1462:1484	the H. erinaceus strain	1462:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	0	54	theme	Room	27:30	arg1	Mutagenesis					58:68	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	11:68	Mutagenesis	58:68	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	6	55	from	erinaceus	1308:1316	arg1	polysaccharides					1284:1298	polysaccharides	1284:1298	polysaccharides from H. erinaceus induced by ARTP mutagenesis	1284:1344	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	2	56	theme	fruiting	416:423	arg1	bodies					425:430	H. erinaceus fruiting bodies	403:430	H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605)	403:545	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	1	57	theme	tumors	308:313	arg1	treatment					281:289	the treatment	277:289	the treatment of neurasthenia, tumors, and digestive diseases	277:337	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	0	58	theme	erinaceus	146:154	arg1	Polysaccharides					156:170	Hericium erinaceus Polysaccharides	137:170	Hericium erinaceus Polysaccharides	137:170	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	7	59	theme	healthy	1557:1563	arg1	development					1573:1583	healthy product development	1557:1583	healthy product development	1557:1583	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	7	60	theme	efficient	1387:1395	arg1	ARTP					1376:1379	ARTP	1376:1379	ARTP	1376:1379	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	7	60	theme	efficient	1387:1395	arg1	method					1411:1416	an efficient and practical method	1384:1416	an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain	1384:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	6	61	theme	20	1259:1260	arg1	%					1261:1261	%	1261:1261	%	1261:1261	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	0	62	theme	Plasma	44:49	arg1	Mutagenesis					58:68	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	11:68	Mutagenesis	58:68	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	4	63	theme	original	907:914	arg1	one					916:918	the original one	903:918	the original one owing to ARTP mutagenesis	903:944	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	0	64	theme	Hericium	137:144	arg1	Polysaccharides					156:170	Hericium erinaceus Polysaccharides	137:170	Hericium erinaceus Polysaccharides	137:170	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	3	65	theme	potential	682:690	arg1	activities					709:718	their potential immunomodulatory activities	676:718	their potential immunomodulatory activities on RAW264.7 macrophages	676:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	4	66	theme	polysaccharide	850:863	arg1	content					865:871	the polysaccharide content	846:871	the polysaccharide content	846:871	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	3	67	theme	RAW264.7	723:730	arg1	macrophages					732:742	RAW264.7 macrophages	723:742	RAW264.7 macrophages	723:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	3	68	theme	immunomodulatory	692:707	arg1	activities					709:718	their potential immunomodulatory activities	676:718	their potential immunomodulatory activities on RAW264.7 macrophages	676:742	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	3	69	theme	physicochemical	605:619	arg1	characteristics					621:635	the physicochemical characteristics	601:635	the physicochemical characteristics of these polysaccharide components	601:670	This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages.
30641994	7	70	theme	breeding	1450:1457	arg1	content					1442:1448	high polysaccharide content breeding	1422:1457	high polysaccharide content breeding of the H. erinaceus strain	1422:1484	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	6	71	theme	NO	1156:1157	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	5	72	from	distribution	968:979	arg1	erinaceus					1054:1062	H. erinaceus	1051:1062	H. erinaceus induced by ARTP mutagenesis	1051:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	0	73	theme	Polysaccharides	156:170	arg1	Characteristics					89:103	Physicochemical Characteristics	73:103	Physicochemical Characteristics	73:103	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	0	73	theme	Polysaccharides	156:170	arg1	Activity					116:123	Immune Activity	109:123	Immune Activity In Vitro	109:132	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	4	74	theme	mutated	810:816	arg1	strain					818:823	mutated strain	810:823	mutated strain increased by 22%	810:840	The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis.
30641994	6	75	theme	IL-6	1160:1163	arg1	activities					1201:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities	1152:1210	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages	1152:1225	The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis.
30641994	1	76	theme	Hericium	234:241	arg1	erinaceus					243:251	Hericium erinaceus	234:251	Hericium erinaceus	234:251	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	7	77	theme	high	1530:1533	arg1	resources					1543:1551	high quality resources	1530:1551	high quality resources	1530:1551	These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
30641994	5	78	theme	components	1035:1044	arg1	distribution					968:979	The molecular weight distribution	947:979	The molecular weight distribution	947:979	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	78	theme	components	1035:1044	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	78	theme	components	1035:1044	arg1	compositions					1004:1015	the monosaccharide compositions	985:1015	the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis	985:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	79	from	that	1126:1129	arg1	distribution					968:979	The molecular weight distribution	947:979	The molecular weight distribution	947:979	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	79	from	that	1126:1129	arg1	different					1111:1119	different	1111:1119	different	1111:1119	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	5	79	from	that	1126:1129	arg1	compositions					1004:1015	the monosaccharide compositions	985:1015	the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis	985:1090	The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one.
30641994	0	80	theme	ARTP	52:55	arg1	Mutagenesis					58:68	Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	11:68	Mutagenesis	58:68	Effects of Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis on Physicochemical Characteristics and Immune Activity In Vitro of Hericium erinaceus Polysaccharides.
30641994	1	81	theme	digestive	320:328	arg1	diseases					330:337	digestive diseases	320:337	digestive diseases	320:337	The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases.
30641994	2	82	theme	ARTP	446:449	arg1	321					511:513	321	511:513	321	511:513	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30641994	2	82	theme	ARTP	446:449	arg1	strain					503:508	ARTP (atmospheric and room temperature plasma) mutagenic strain	446:508	ARTP (atmospheric and room temperature plasma) mutagenic strain (321)	446:514	Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively.
30282014	4	0	theme	study	764:768	arg1	part					752:755	The second part	741:755	The second part of the study	741:768	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	6	1	theme	foam	1187:1190	arg1	tray					1192:1195	the starch foam tray	1176:1195	the starch foam tray	1176:1195	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	1	2	theme	bio-based	222:230	arg1	carbonate					240:248	bio-based calcium carbonate	222:248	bio-based calcium carbonate	222:248	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	7	3	theme	commercial	1371:1380	arg1	CaCO3					1382:1386	commercial CaCO3	1371:1386	commercial CaCO3	1371:1386	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	2	4	theme	X-ray	516:520	arg1	spectroscopy					522:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy	443:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX)	443:539	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	4	theme	X-ray	516:520	arg1	EDX					536:538	EDX	536:538	EDX	536:538	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	7	5	theme	whiteness	1329:1337	arg1	values					1339:1344	the whiteness values	1325:1344	the whiteness values of starch foam tray	1325:1364	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	4	6	theme	commercial	942:951	arg1	CaCO3					953:957	commercial CaCO3	942:957	commercial CaCO3	942:957	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	1	7	theme	carbonate	240:248	arg1	source					212:217	a source	210:217	a source of bio-based calcium carbonate which can be used as a filler in starch foam tray	210:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	7	theme	carbonate	240:248	arg1	filler					273:278	a filler	271:278	a filler in starch foam tray	271:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	7	theme	carbonate	240:248	arg1	macroalga					176:184	The calcified green macroalga	156:184	The calcified green macroalga	156:184	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	7	8	theme	tray	1361:1364	arg1	values					1339:1344	the whiteness values	1325:1344	the whiteness values of starch foam tray	1325:1364	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	7	8	theme	tray	1361:1364	arg1	changes					1289:1295	more significant changes	1272:1295	more significant changes in the color parameters	1272:1319	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	2	9	theme	Scanning	545:552	arg1	SEM					575:577	SEM	575:577	SEM	575:577	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	9	theme	Scanning	545:552	arg1	microscopy					563:572	Scanning election microscopy	545:572	Scanning election microscopy (SEM)	545:578	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	3	10	theme	particles	621:629	arg1	rod-like					635:642	rod-like	635:642	rod-like	635:642	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	3	10	theme	particles	621:629	arg1	morphology					585:594	The morphology	581:594	The morphology of the macroalgal powder particles	581:629	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	7	11	theme	starch	1349:1354	arg1	tray					1361:1364	starch foam tray	1349:1364	starch foam tray	1349:1364	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	7	12	theme	color	1304:1308	arg1	parameters					1310:1319	the color parameters	1300:1319	the color parameters	1300:1319	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	1	13	theme	green	170:174	arg1	source					212:217	a source	210:217	a source of bio-based calcium carbonate which can be used as a filler in starch foam tray	210:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	13	theme	green	170:174	arg1	macroalga					176:184	The calcified green macroalga	156:184	The calcified green macroalga	156:184	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	13	theme	green	170:174	arg1	macroloba					196:204	macroloba	196:204	macroloba	196:204	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	4	14	theme	H.	821:822	arg1	macroloba					824:832	H. macroloba	821:832	H. macroloba	821:832	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	3	15	theme	organic	648:654	arg1	materials					656:664	organic materials	648:664	organic materials (e.g. polysaccharide)	648:686	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	2	16	theme	X-ray	473:477	arg1	diffraction					479:489	X-ray diffraction	473:489	X-ray diffraction (XRD)	473:495	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	16	theme	X-ray	473:477	arg1	spectrometry					452:463	infrared spectrometry	443:463	infrared spectrometry (FTIR)	443:470	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	16	theme	X-ray	473:477	arg1	XRD					492:494	XRD	492:494	XRD	492:494	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	7	17	theme	significant	1277:1287	arg1	changes					1289:1295	more significant changes	1272:1295	more significant changes in the color parameters	1272:1319	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	6	18	theme	commercial	1216:1225	arg1	CaCO3					1227:1231	commercial CaCO3	1216:1231	commercial CaCO3	1216:1231	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	2	19	theme	calcium	367:373	arg1	carbonate					375:383	calcium carbonate	367:383	calcium carbonate of this species	367:399	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	20	theme	Energy	498:503	arg1	spectroscopy					522:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy	443:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX)	443:539	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	20	theme	Energy	498:503	arg1	EDX					536:538	EDX	536:538	EDX	536:538	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	6	21	theme	macroalgal	1123:1132	arg1	powder					1134:1139	the macroalgal powder	1119:1139	the macroalgal powder	1119:1139	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	0	22	theme	composite	127:135	arg1	trays					149:153	composite starch foam trays	127:153	composite starch foam trays	127:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	4	23	theme	carbonate	806:814	arg1	effects					787:793	the effects	783:793	the effects of calcium carbonate from H. macroloba on the properties of starch foam tray	783:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	7	24	theme	macroalgal	1247:1256	arg1	powder					1258:1263	the macroalgal powder	1243:1263	the macroalgal powder	1243:1263	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	5	25	with	trays	1002:1006	arg1	powder					1024:1029	macroalgal powder	1013:1029	macroalgal powder	1013:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	3	26	theme	aragonite	711:719	arg1	carbonate					700:708	calcium carbonate	692:708	calcium carbonate (aragonite form)	692:725	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	3	26	theme	aragonite	711:719	arg1	form					721:724	aragonite form	711:724	aragonite form	711:724	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	0	27	theme	foam	144:147	arg1	trays					149:153	composite starch foam trays	127:153	composite starch foam trays	127:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	28	contain	had	1031:1033	arg1	trays					1002:1006	the composites starch foam trays	975:1006	the composites starch foam trays with macroalgal powder	975:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	5	28	contain	had	1031:1033	arg2	strength					1049:1056	better impact strength	1035:1056	better impact strength	1035:1056	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	1	29	theme	starch	283:288	arg1	tray					295:298	starch foam tray	283:298	starch foam tray	283:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	7	30	theme	foam	1356:1359	arg1	tray					1361:1364	starch foam tray	1349:1364	starch foam tray	1349:1364	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	0	31	theme	macroloba	65:73	arg1	Decaisne					75:82	Halimeda macroloba Decaisne	56:82	Halimeda macroloba Decaisne	56:82	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	31	theme	macroloba	65:73	arg1	macroalga					45:53	calcified green macroalga	29:53	calcified green macroalga (Halimeda macroloba Decaisne)	29:83	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	32	from	Comparison	0:9	arg1	properties					113:122	the properties	109:122	the properties of composite starch foam trays	109:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	33	theme	composites	979:988	arg1	trays					1002:1006	the composites starch foam trays	975:1006	the composites starch foam trays with macroalgal powder	975:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	4	34	theme	starch	855:860	arg1	tray					867:870	starch foam tray	855:870	starch foam tray	855:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	2	35	theme	election	554:561	arg1	SEM					575:577	SEM	575:577	SEM	575:577	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	35	theme	election	554:561	arg1	microscopy					563:572	Scanning election microscopy	545:572	Scanning election microscopy (SEM)	545:578	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	0	36	theme	commercial	89:98	arg1	CaCO3					100:104	commercial CaCO3	89:104	commercial CaCO3	89:104	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	37	theme	foam	997:1000	arg1	trays					1002:1006	the composites starch foam trays	975:1006	the composites starch foam trays with macroalgal powder	975:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	4	38	theme	starch	913:918	arg1	tray					925:928	starch foam tray	913:928	starch foam tray filled with commercial CaCO3	913:957	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	2	39	theme	infrared	443:450	arg1	diffraction					479:489	X-ray diffraction	473:489	X-ray diffraction (XRD)	473:495	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	39	theme	infrared	443:450	arg1	FTIR					466:469	FTIR	466:469	FTIR	466:469	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	39	theme	infrared	443:450	arg1	spectrometry					452:463	infrared spectrometry	443:463	infrared spectrometry (FTIR)	443:470	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	5	40	theme	starch/commercial	1063:1079	arg1	trays					1102:1106	starch/commercial CaCO3 composite foam trays	1063:1106	starch/commercial CaCO3 composite foam trays	1063:1106	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	4	41	theme	second	745:750	arg1	part					752:755	The second part	741:755	The second part of the study	741:768	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	4	42	theme	tray	925:928	arg1	properties					899:908	the properties	895:908	the properties of starch foam tray filled with commercial CaCO3	895:957	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	5	43	theme	composite	1087:1095	arg1	trays					1102:1106	starch/commercial CaCO3 composite foam trays	1063:1106	starch/commercial CaCO3 composite foam trays	1063:1106	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	2	44	dep	composition	338:348	arg1	the					334:336	the	334:336	the	334:336	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	5	45	theme	better	1035:1040	arg1	strength					1049:1056	better impact strength	1035:1056	better impact strength	1035:1056	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	2	46	theme	species	393:399	arg1	carbonate					375:383	calcium carbonate	367:383	calcium carbonate of this species	367:399	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	0	47	theme	green	39:43	arg1	Decaisne					75:82	Halimeda macroloba Decaisne	56:82	Halimeda macroloba Decaisne	56:82	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	47	theme	green	39:43	arg1	macroalga					45:53	calcified green macroalga	29:53	calcified green macroalga (Halimeda macroloba Decaisne)	29:83	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	48	from	properties	113:122	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.	0:154	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	3	49	theme	powder	614:619	arg1	particles					621:629	the macroalgal powder particles	599:629	the macroalgal powder particles	599:629	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	1	50	from	filler	273:278	arg1	tray					295:298	starch foam tray	283:298	starch foam tray	283:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	5	51	theme	macroalgal	1013:1022	arg1	powder					1024:1029	macroalgal powder	1013:1029	macroalgal powder	1013:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	6	52	theme	starch	1180:1185	arg1	tray					1192:1195	the starch foam tray	1176:1195	the starch foam tray	1176:1195	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	1	53	theme	calcium	232:238	arg1	carbonate					240:248	bio-based calcium carbonate	222:248	bio-based calcium carbonate	222:248	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	54	used	used	263:266	arg2	filler					273:278	a filler	271:278	a filler in starch foam tray	271:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	54	used	used	263:266	arg2	source					212:217	a source	210:217	a source of bio-based calcium carbonate which can be used as a filler in starch foam tray	210:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	54	used	used	263:266	arg2	macroalga					176:184	The calcified green macroalga	156:184	The calcified green macroalga	156:184	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	2	55	theme	dispersive	505:514	arg1	spectroscopy					522:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy	443:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX)	443:539	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	55	theme	dispersive	505:514	arg1	EDX					536:538	EDX	536:538	EDX	536:538	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	6	56	theme	thermal	1154:1160	arg1	properties					1162:1171	the thermal properties	1150:1171	the thermal properties of the starch foam tray	1150:1195	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	1	57	theme	calcified	160:168	arg1	source					212:217	a source	210:217	a source of bio-based calcium carbonate which can be used as a filler in starch foam tray	210:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	57	theme	calcified	160:168	arg1	macroalga					176:184	The calcified green macroalga	156:184	The calcified green macroalga	156:184	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	1	57	theme	calcified	160:168	arg1	macroloba					196:204	macroloba	196:204	macroloba	196:204	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	4	58	theme	tray	867:870	arg1	properties					841:850	the properties	837:850	the properties of starch foam tray	837:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	4	59	from	macroloba	824:832	arg1	effects					787:793	the effects	783:793	the effects of calcium carbonate from H. macroloba on the properties of starch foam tray	783:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	4	59	from	macroloba	824:832	arg1	carbonate					806:814	calcium carbonate	798:814	calcium carbonate from H. macroloba	798:832	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	6	60	theme	tray	1192:1195	arg1	properties					1162:1171	the thermal properties	1150:1171	the thermal properties of the starch foam tray	1150:1195	Moreover, the macroalgal powder affected the thermal properties of the starch foam tray equally as well as commercial CaCO3.
30282014	3	61	theme	calcium	692:698	arg1	carbonate					700:708	calcium carbonate	692:708	calcium carbonate (aragonite form)	692:725	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	3	61	theme	calcium	692:698	arg1	form					721:724	aragonite form	711:724	aragonite form	711:724	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	0	62	theme	starch	137:142	arg1	trays					149:153	composite starch foam trays	127:153	composite starch foam trays	127:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	63	theme	trays	149:153	arg1	properties					113:122	the properties	109:122	the properties of composite starch foam trays	109:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	4	64	theme	calcium	798:804	arg1	carbonate					806:814	calcium carbonate	798:814	calcium carbonate from H. macroloba	798:832	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	0	65	theme	Halimeda	56:63	arg1	Decaisne					75:82	Halimeda macroloba Decaisne	56:82	Halimeda macroloba Decaisne	56:82	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	65	theme	Halimeda	56:63	arg1	macroalga					45:53	calcified green macroalga	29:53	calcified green macroalga (Halimeda macroloba Decaisne)	29:83	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	4	66	from	effects	787:793	arg1	macroloba					824:832	H. macroloba	821:832	H. macroloba	821:832	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	4	66	from	effects	787:793	arg1	properties					841:850	the properties	837:850	the properties of starch foam tray	837:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	4	67	theme	foam	862:865	arg1	tray					867:870	starch foam tray	855:870	starch foam tray	855:870	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	0	68	from	effects	18:24	arg1	properties					113:122	the properties	109:122	the properties of composite starch foam trays	109:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	1	69	theme	foam	290:293	arg1	tray					295:298	starch foam tray	283:298	starch foam tray	283:298	The calcified green macroalga, Halimeda macroloba, is a source of bio-based calcium carbonate which can be used as a filler in starch foam tray.
30282014	3	70	dep	polysaccharide	672:685	arg1	e.g.					667:670	e.g.	667:670	e.g.	667:670	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	7	71	from	values	1339:1344	arg1	parameters					1310:1319	the color parameters	1300:1319	the color parameters	1300:1319	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	0	72	theme	CaCO3	100:104	arg1	effects					18:24	the effects	14:24	the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays	14:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	73	theme	starch	990:995	arg1	trays					1002:1006	the composites starch foam trays	975:1006	the composites starch foam trays with macroalgal powder	975:1029	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	2	74	theme	spectrometry	452:463	arg1	spectroscopy					522:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy	443:533	infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX)	443:539	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	74	theme	spectrometry	452:463	arg1	EDX					536:538	EDX	536:538	EDX	536:538	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	75	theme	study	327:331	arg1	part					314:317	the first part	304:317	the first part of this study	304:331	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	76	theme	carbonate	375:383	arg1	composition					338:348	composition	338:348	composition	338:348	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	2	76	theme	carbonate	375:383	arg1	structure					354:362	structure	354:362	structure	354:362	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	0	77	theme	effects	18:24	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.	0:154	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	78	theme	CaCO3	1081:1085	arg1	trays					1102:1106	starch/commercial CaCO3 composite foam trays	1063:1106	starch/commercial CaCO3 composite foam trays	1063:1106	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	4	79	theme	foam	920:923	arg1	tray					925:928	starch foam tray	913:928	starch foam tray filled with commercial CaCO3	913:957	The second part of the study investigated the effects of calcium carbonate from H. macroloba on the properties of starch foam tray and compared them with the properties of starch foam tray filled with commercial CaCO3.
30282014	0	80	theme	calcified	29:37	arg1	Decaisne					75:82	Halimeda macroloba Decaisne	56:82	Halimeda macroloba Decaisne	56:82	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	0	80	theme	calcified	29:37	arg1	macroalga					45:53	calcified green macroalga	29:53	calcified green macroalga (Halimeda macroloba Decaisne)	29:83	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	81	theme	foam	1097:1100	arg1	trays					1102:1106	starch/commercial CaCO3 composite foam trays	1063:1106	starch/commercial CaCO3 composite foam trays	1063:1106	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	7	82	from	changes	1289:1295	arg1	parameters					1310:1319	the color parameters	1300:1319	the color parameters	1300:1319	However, the macroalgal powder caused more significant changes in the color parameters and the whiteness values of starch foam tray than commercial CaCO3.
30282014	0	83	theme	macroalga	45:53	arg1	effects					18:24	the effects	14:24	the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays	14:153	Comparison of the effects of calcified green macroalga (Halimeda macroloba Decaisne) and commercial CaCO3 on the properties of composite starch foam trays.
30282014	5	84	theme	impact	1042:1047	arg1	strength					1049:1056	better impact strength	1035:1056	better impact strength	1035:1056	Interestingly, the composites starch foam trays with macroalgal powder had better impact strength than starch/commercial CaCO3 composite foam trays.
30282014	2	85	dep	Fourier	425:431	arg1	transform					433:441	transform	433:441	transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM)	433:578	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30282014	3	86	theme	macroalgal	603:612	arg1	particles					621:629	the macroalgal powder particles	599:629	the macroalgal powder particles	599:629	The morphology of the macroalgal powder particles was rod-like and organic materials (e.g. polysaccharide) and calcium carbonate (aragonite form) were present.
30282014	2	87	theme	first	308:312	arg1	part					314:317	the first part	304:317	the first part of this study	304:331	In the first part of this study, the composition and structure of calcium carbonate of this species were investigated using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX) and Scanning election microscopy (SEM).
30910676	5	0	theme	radical	851:857	arg1	activities					859:868	higher scavenging radical activities	833:868	higher scavenging radical activities	833:868	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	6	1	theme	α-glucosidase	1010:1022	arg1	activity					1024:1031	α-glucosidase activity	1010:1031	α-glucosidase activity in a noncompetitive inhibition type	1010:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	4	2	theme	selenyl	770:776	arg1	groups					778:783	selenyl groups	770:783	selenyl groups	770:783	FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups.
30910676	2	3	theme	2419 μg/g	366:374	arg1	content					355:361	the selenium content	342:361	the selenium content of 2419 μg/g	342:374	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	0	4	from	characterization	16:31	arg1	pallidum					118:125	Sargassum pallidum	108:125	Sargassum pallidum	108:125	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	1	5	from	effects	168:174	arg1	properties					226:235	physicochemical and biological properties	195:235	physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum	195:293	This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum.
30910676	3	6	theme	monosaccharide	562:575	arg1	composition					577:587	monosaccharide composition	562:587	monosaccharide composition	562:587	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	4	7	theme	FT-IR	650:654	arg1	spectroscopy					656:667	FT-IR spectroscopy	650:667	FT-IR spectroscopy	650:667	FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups.
30910676	8	8	theme	new	1236:1238	arg1	ingredient					1263:1272	a new selenium-complementary ingredient	1234:1272	a new selenium-complementary ingredient in functional foods	1234:1292	These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
30910676	7	9	theme	IC50	1074:1077	arg1	1.579					1133:1137	1.579	1133:1137	1.579	1133:1137	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	9	theme	IC50	1074:1077	arg1	values					1079:1084	The IC50 values	1070:1084	The IC50 values of SPP, Se-SPP and acarbose	1070:1112	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	9	theme	IC50	1074:1077	arg1	2.742 mg/mL					1150:1160	2.742 mg/mL	1150:1160	2.742 mg/mL	1150:1160	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	9	theme	IC50	1074:1077	arg1	0.896					1140:1144	0.896	1140:1144	0.896	1140:1144	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	6	10	theme	inhibitory	989:998	arg1	effect					1000:1005	inhibitory effect	989:1005	inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type	989:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	8	11	used	used	1218:1221	arg2	Se-SPP					1204:1209	Se-SPP	1204:1209	Se-SPP	1204:1209	These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
30910676	3	12	theme	surface	611:617	arg1	morphology					619:628	surface morphology	611:628	surface morphology	611:628	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	0	13	from	activities	63:72	arg1	pallidum					118:125	Sargassum pallidum	108:125	Sargassum pallidum	108:125	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	2	14	theme	selenized	300:308	arg1	derivative					310:319	The selenized derivative	296:319	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g	296:374	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	1	15	theme	physicochemical	195:209	arg1	properties					226:235	physicochemical and biological properties	195:235	physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum	195:293	This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum.
30910676	1	16	theme	selenylation	179:190	arg1	effects					168:174	the effects	164:174	the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum	164:293	This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum.
30910676	8	17	theme	selenium-complementary	1240:1261	arg1	ingredient					1263:1272	a new selenium-complementary ingredient	1234:1272	a new selenium-complementary ingredient in functional foods	1234:1292	These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
30910676	3	18	from	changes	529:535	arg1	composition					549:559	chemical composition	540:559	chemical composition	540:559	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	18	from	changes	529:535	arg1	composition					577:587	monosaccharide composition	562:587	monosaccharide composition	562:587	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	18	from	changes	529:535	arg1	weight					600:605	molecular weight	590:605	molecular weight	590:605	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	18	from	changes	529:535	arg1	morphology					619:628	surface morphology	611:628	surface morphology	611:628	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	2	19	theme	selenite/dilute	402:416	arg1	method					430:435	sodium selenite/dilute nitric acid method	395:435	sodium selenite/dilute nitric acid method	395:435	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	8	20	theme	functional	1277:1286	arg1	foods					1288:1292	functional foods	1277:1292	functional foods	1277:1292	These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
30910676	5	21	theme	higher	833:838	arg1	activities					859:868	higher scavenging radical activities	833:868	higher scavenging radical activities	833:868	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	3	22	theme	polysaccharides	633:647	arg1	composition					549:559	chemical composition	540:559	chemical composition	540:559	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	22	theme	polysaccharides	633:647	arg1	composition					577:587	monosaccharide composition	562:587	monosaccharide composition	562:587	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	22	theme	polysaccharides	633:647	arg1	weight					600:605	molecular weight	590:605	molecular weight	590:605	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	3	22	theme	polysaccharides	633:647	arg1	morphology					619:628	surface morphology	611:628	surface morphology	611:628	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	2	23	theme	sodium	395:400	arg1	method					430:435	sodium selenite/dilute nitric acid method	395:435	sodium selenite/dilute nitric acid method	395:435	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	0	24	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	25	from	pallidum	118:125	arg1	antioxidant					34:44	antioxidant	34:44	antioxidant	34:44	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	25	from	pallidum	118:125	arg1	activities					63:72	hypoglycemic activities	50:72	hypoglycemic activities	50:72	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	25	from	pallidum	118:125	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	25	from	pallidum	118:125	arg1	polysaccharides					87:101	selenized polysaccharides	77:101	selenized polysaccharides from Sargassum pallidum	77:125	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	5	26	theme	native	912:917	arg1	SPP					919:921	native SPP	912:921	native SPP	912:921	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	1	27	theme	biological	215:224	arg1	properties					226:235	physicochemical and biological properties	195:235	physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum	195:293	This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum.
30910676	3	28	theme	molecular	590:598	arg1	weight					600:605	molecular weight	590:605	molecular weight	590:605	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	2	29	theme	SPP	324:326	arg1	derivative					310:319	The selenized derivative	296:319	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g	296:374	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	0	30	theme	hypoglycemic	50:61	arg1	activities					63:72	hypoglycemic activities	50:72	hypoglycemic activities	50:72	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	4	31	theme	groups	778:783	arg1	substitution					754:765	the substitution	750:765	the substitution of selenyl groups	750:783	FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups.
30910676	5	32	theme	Bioactivity	786:796	arg1	assay					798:802	Bioactivity assay	786:802	Bioactivity assay	786:802	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	2	33	theme	nitric	418:423	arg1	method					430:435	sodium selenite/dilute nitric acid method	395:435	sodium selenite/dilute nitric acid method	395:435	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	6	34	theme	inhibition	1053:1062	arg1	type					1064:1067	a noncompetitive inhibition type	1036:1067	a noncompetitive inhibition type	1036:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	3	35	theme	Physicochemical	438:452	arg1	characterization					454:469	Physicochemical characterization	438:469	Physicochemical characterization	438:469	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	7	36	theme	acarbose	1105:1112	arg1	1.579					1133:1137	1.579	1133:1137	1.579	1133:1137	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	36	theme	acarbose	1105:1112	arg1	values					1079:1084	The IC50 values	1070:1084	The IC50 values of SPP, Se-SPP and acarbose	1070:1112	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	36	theme	acarbose	1105:1112	arg1	2.742 mg/mL					1150:1160	2.742 mg/mL	1150:1160	2.742 mg/mL	1150:1160	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	36	theme	acarbose	1105:1112	arg1	0.896					1140:1144	0.896	1140:1144	0.896	1140:1144	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	2	37	theme	acid	425:428	arg1	method					430:435	sodium selenite/dilute nitric acid method	395:435	sodium selenite/dilute nitric acid method	395:435	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	5	38	theme	ion	882:884	arg1	activities					896:905	ferrous ion chelating activities	874:905	ferrous ion chelating activities	874:905	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	6	39	theme	noncompetitive	1038:1051	arg1	type					1064:1067	a noncompetitive inhibition type	1036:1067	a noncompetitive inhibition type	1036:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	5	40	theme	scavenging	840:849	arg1	activities					859:868	higher scavenging radical activities	833:868	higher scavenging radical activities	833:868	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	3	41	theme	chemical	540:547	arg1	composition					549:559	chemical composition	540:559	chemical composition	540:559	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	5	42	theme	chelating	886:894	arg1	activities					896:905	ferrous ion chelating activities	874:905	ferrous ion chelating activities	874:905	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	8	43	from	ingredient	1263:1272	arg1	foods					1288:1292	functional foods	1277:1292	functional foods	1277:1292	These results suggest that Se-SPP can be used to develop a new selenium-complementary ingredient in functional foods.
30910676	7	44	theme	Se-SPP	1094:1099	arg1	1.579					1133:1137	1.579	1133:1137	1.579	1133:1137	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	44	theme	Se-SPP	1094:1099	arg1	values					1079:1084	The IC50 values	1070:1084	The IC50 values of SPP, Se-SPP and acarbose	1070:1112	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	44	theme	Se-SPP	1094:1099	arg1	2.742 mg/mL					1150:1160	2.742 mg/mL	1150:1160	2.742 mg/mL	1150:1160	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	44	theme	Se-SPP	1094:1099	arg1	0.896					1140:1144	0.896	1140:1144	0.896	1140:1144	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	2	45	theme	selenium	346:353	arg1	content					355:361	the selenium content	342:361	the selenium content of 2419 μg/g	342:374	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30910676	6	46	from	effect	1000:1005	arg1	activity					1024:1031	α-glucosidase activity	1010:1031	α-glucosidase activity in a noncompetitive inhibition type	1010:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	5	47	theme	ferrous	874:880	arg1	activities					896:905	ferrous ion chelating activities	874:905	ferrous ion chelating activities	874:905	Bioactivity assay showed that Se-SPP exhibited higher scavenging radical activities and ferrous ion chelating activities than native SPP.
30910676	4	48	theme	new	683:685	arg1	peak					698:701	a new absorption peak	681:701	a new absorption peak	681:701	FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups.
30910676	7	49	theme	SPP	1089:1091	arg1	1.579					1133:1137	1.579	1133:1137	1.579	1133:1137	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	49	theme	SPP	1089:1091	arg1	values					1079:1084	The IC50 values	1070:1084	The IC50 values of SPP, Se-SPP and acarbose	1070:1112	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	49	theme	SPP	1089:1091	arg1	2.742 mg/mL					1150:1160	2.742 mg/mL	1150:1160	2.742 mg/mL	1150:1160	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	7	49	theme	SPP	1089:1091	arg1	0.896					1140:1144	0.896	1140:1144	0.896	1140:1144	The IC50 values of SPP, Se-SPP and acarbose were determined as 1.579, 0.896 and 2.742 mg/mL, respectively.
30910676	0	50	theme	polysaccharides	87:101	arg1	antioxidant					34:44	antioxidant	34:44	antioxidant	34:44	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	50	theme	polysaccharides	87:101	arg1	activities					63:72	hypoglycemic activities	50:72	hypoglycemic activities	50:72	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	50	theme	polysaccharides	87:101	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	1	51	theme	polysaccharide	240:253	arg1	properties					226:235	physicochemical and biological properties	195:235	physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum	195:293	This study was carried out to study the effects of selenylation on physicochemical and biological properties of polysaccharide (SPP) extracted from Sargassum pallidum.
30910676	3	52	theme	selenylation	486:497	arg1	modification					499:510	selenylation modification	486:510	selenylation modification	486:510	Physicochemical characterization indicated that selenylation modification resulted in some changes in chemical composition, monosaccharide composition, molecular weight and surface morphology of polysaccharides.
30910676	0	53	theme	selenized	77:85	arg1	polysaccharides					87:101	selenized polysaccharides	77:101	selenized polysaccharides from Sargassum pallidum	77:125	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	0	54	from	antioxidant	34:44	arg1	pallidum					118:125	Sargassum pallidum	108:125	Sargassum pallidum	108:125	Physicochemical characterization, antioxidant and hypoglycemic activities of selenized polysaccharides from Sargassum pallidum.
30910676	4	55	theme	absorption	687:696	arg1	peak					698:701	a new absorption peak	681:701	a new absorption peak	681:701	FT-IR spectroscopy showed that a new absorption peak appeared at 675 cm-1 in Se-SPP probably due to the substitution of selenyl groups.
30910676	6	56	from	activity	1024:1031	arg1	type					1064:1067	a noncompetitive inhibition type	1036:1067	a noncompetitive inhibition type	1036:1067	Compared with SPP and acarbose, Se-SPP showed more significantly inhibitory effect on α-glucosidase activity in a noncompetitive inhibition type.
30910676	2	57	with	derivative	310:319	arg1	content					355:361	the selenium content	342:361	the selenium content of 2419 μg/g	342:374	The selenized derivative of SPP (Se-SPP) with the selenium content of 2419 μg/g was synthesized by sodium selenite/dilute nitric acid method.
30536035	2	0	theme	sugar	455:459	arg1	contents					461:468	their total sugar contents	443:468	their total sugar contents	443:468	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	1	from	treatment	326:334	arg1	GFP-4					339:343	GFP-4	339:343	GFP-4	339:343	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	2	theme	heat	164:167	arg1	treatment					169:177	heat treatment	164:177	heat treatment	164:177	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	3	theme	HepG2	793:797	arg1	cells					799:803	HepG2 cells	793:803	HepG2 cells	793:803	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	4	theme	%	493:493	arg1	results					728:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	0	5	from	Effects	0:6	arg1	Activity					74:81	Antitumor Activity	64:81	Antitumor Activity	64:81	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	5	from	Effects	0:6	arg1	Characteristics					44:58	Structural Characteristics	33:58	Structural Characteristics	33:58	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	2	6	theme	chromatography	556:569	arg1	results					627:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	3	7	from	Grifola	913:919	arg1	polysaccharides					892:906	polysaccharides	892:906	polysaccharides from Grifola frondosa	892:928	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	0	8	theme	Polysaccharides	86:100	arg1	Activity					74:81	Antitumor Activity	64:81	Antitumor Activity	64:81	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	8	theme	Polysaccharides	86:100	arg1	Characteristics					44:58	Structural Characteristics	33:58	Structural Characteristics	33:58	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	9	from	Characteristics	44:58	arg1	frondosa					115:122	frondosa	115:122	frondosa	115:122	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	2	10	theme	ion	552:554	arg1	IC					572:573	IC	572:573	IC	572:573	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	10	theme	ion	552:554	arg1	chromatography					556:569	ion chromatography	552:569	ion chromatography (IC)	552:574	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	11	theme	JC-1	723:726	arg1	results					728:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	12	theme	80 °C	320:324	arg1	treatment					326:334	80 °C treatment	320:334	80 °C treatment on GFP-4	320:343	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	3	13	theme	heat	865:868	arg1	treatment					870:878	heat treatment	865:878	heat treatment at 80 °C on polysaccharides from Grifola frondosa	865:928	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	2	14	theme	infrared	598:605	arg1	FTIR					621:624	FTIR	621:624	FTIR	621:624	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	14	theme	infrared	598:605	arg1	spectroscopy					607:618	Fourier-transform infrared spectroscopy	580:618	Fourier-transform infrared spectroscopy (FTIR)	580:625	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	15	from	effects	153:159	arg1	activity					232:239	in vitro antitumor activity	213:239	in vitro antitumor activity	213:239	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	15	from	effects	153:159	arg1	characteristics					193:207	structural characteristics	182:207	structural characteristics	182:207	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	16	from	effects	782:788	arg1	cells					799:803	HepG2 cells	793:803	HepG2 cells	793:803	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	17	dep	GFP-4	283:287	arg1	extracted					290:298	extracted	290:298	extracted at 4 °C	290:306	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	18	theme	treatment	169:177	arg1	effects					153:159	the effects	149:159	the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa	149:280	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	19	theme	Fourier-transform	580:596	arg1	FTIR					621:624	FTIR	621:624	FTIR	621:624	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	19	theme	Fourier-transform	580:596	arg1	spectroscopy					607:618	Fourier-transform infrared spectroscopy	580:618	Fourier-transform infrared spectroscopy (FTIR)	580:625	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	20	dep	GFP-80	350:355	arg1	extracted					358:366	extracted	358:366	extracted at 80 °C	358:375	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	21	theme	MTT	715:717	arg1	results					728:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results	475:734	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	22	theme	structural	182:191	arg1	characteristics					193:207	structural characteristics	182:207	structural characteristics	182:207	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	23	theme	composition	410:420	arg1	analysis					422:429	the chemical composition analysis	397:429	the chemical composition analysis	397:429	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	0	24	theme	Treatment	16:24	arg1	Effects					0:6	Effects	0:6	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.	0:123	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	2	25	theme	chromatography	528:541	arg1	results					627:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	26	theme	spectroscopy	607:618	arg1	results					627:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results	496:633	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	3	27	theme	antitumor	980:988	arg1	effects					990:996	their antitumor effects	974:996	their antitumor effects	974:996	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	0	28	theme	Heat	11:14	arg1	Treatment					16:24	Heat Treatment	11:24	Heat Treatment	11:24	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	2	29	theme	gel-permeation	513:526	arg1	HPGPC					544:548	HPGPC	544:548	HPGPC	544:548	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	29	theme	gel-permeation	513:526	arg1	chromatography					528:541	high-performance gel-permeation chromatography	496:541	high-performance gel-permeation chromatography (HPGPC)	496:549	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	30	dep	GFP-4-80	310:317	arg1	treatment					326:334	80 °C treatment	320:334	80 °C treatment on GFP-4	320:343	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	31	theme	in	213:214	arg1	activity					232:239	in vitro antitumor activity	213:239	in vitro antitumor activity	213:239	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	32	theme	total	449:453	arg1	contents					461:468	their total sugar contents	443:468	their total sugar contents	443:468	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	3	33	dep	Grifola	913:919	arg1	frondosa					921:928	Grifola frondosa	913:928	Grifola frondosa	913:928	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	2	34	theme	high-performance	496:511	arg1	HPGPC					544:548	HPGPC	544:548	HPGPC	544:548	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	34	theme	high-performance	496:511	arg1	chromatography					528:541	high-performance gel-permeation chromatography	496:541	high-performance gel-permeation chromatography (HPGPC)	496:549	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	0	35	from	Activity	74:81	arg1	frondosa					115:122	frondosa	115:122	frondosa	115:122	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	36	dep	Characteristics	44:58	arg1	the					29:31	the	29:31	the	29:31	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	1	37	from	frondosa	273:280	arg1	polysaccharides					244:258	polysaccharides	244:258	polysaccharides from Grifola frondosa	244:280	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	37	from	frondosa	273:280	arg1	activity					232:239	in vitro antitumor activity	213:239	in vitro antitumor activity	213:239	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	37	from	frondosa	273:280	arg1	characteristics					193:207	structural characteristics	182:207	structural characteristics	182:207	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	38	theme	chemical	401:408	arg1	analysis					422:429	the chemical composition analysis	397:429	the chemical composition analysis	397:429	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	1	39	theme	antitumor	222:230	arg1	activity					232:239	in vitro antitumor activity	213:239	in vitro antitumor activity	213:239	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	40	dep	%	493:493	arg1	demonstrated					635:646	demonstrated	635:646	demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment	635:712	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	0	41	theme	Structural	33:42	arg1	Characteristics					44:58	Structural Characteristics	33:58	Structural Characteristics	33:58	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	42	from	frondosa	115:122	arg1	Activity					74:81	Antitumor Activity	64:81	Antitumor Activity	64:81	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	42	from	frondosa	115:122	arg1	Polysaccharides					86:100	Polysaccharides	86:100	Polysaccharides from Grifola frondosa	86:122	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	0	42	from	frondosa	115:122	arg1	Characteristics					44:58	Structural Characteristics	33:58	Structural Characteristics	33:58	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	3	43	from	treatment	870:878	arg1	polysaccharides					892:906	polysaccharides	892:906	polysaccharides from Grifola frondosa	892:928	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	1	44	from	activity	232:239	arg1	frondosa					273:280	frondosa	273:280	frondosa	273:280	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	3	45	from	80 °C	883:887	arg1	treatment					870:878	heat treatment	865:878	heat treatment at 80 °C on polysaccharides from Grifola frondosa	865:928	Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
30536035	2	46	theme	inhibitory	771:780	arg1	effects					782:788	higher inhibitory effects	764:788	higher inhibitory effects on HepG2 cells	764:803	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	47	theme	heat	699:702	arg1	treatment					704:712	80 °C heat treatment	693:712	80 °C heat treatment	693:712	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	2	48	theme	higher	764:769	arg1	effects					782:788	higher inhibitory effects	764:788	higher inhibitory effects on HepG2 cells	764:803	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
30536035	0	49	theme	Antitumor	64:72	arg1	Activity					74:81	Antitumor Activity	64:81	Antitumor Activity	64:81	Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa.
30536035	1	50	from	characteristics	193:207	arg1	frondosa					273:280	frondosa	273:280	frondosa	273:280	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	51	theme	polysaccharides	244:258	arg1	activity					232:239	in vitro antitumor activity	213:239	in vitro antitumor activity	213:239	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	51	theme	polysaccharides	244:258	arg1	characteristics					193:207	structural characteristics	182:207	structural characteristics	182:207	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	1	52	dep	in	213:214	arg1	vitro					216:220	vitro	216:220	vitro	216:220	This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa.
30536035	2	53	theme	80 °C	693:697	arg1	treatment					704:712	80 °C heat treatment	693:712	80 °C heat treatment	693:712	GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80.
31016560	0	0	theme	ion	97:99	arg1	spectrometry					111:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	4	1	theme	liquid	675:680	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	1	theme	liquid	675:680	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	2	2	theme	glycosaminoglycans	329:346	arg1	class					320:324	a structurally complex class	297:324	a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	297:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	2	2	theme	glycosaminoglycans	329:346	arg1	/heparin					285:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	5	3	located	detected	885:892	arg2	disaccharides					861:873	the four rare N-unsubstituted disaccharides	831:873	the four rare N-unsubstituted disaccharides	831:873	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	5	3	located	detected	885:892	arg1	quantities					909:918	significant quantities	897:918	significant quantities	897:918	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	0	4	theme	chromatography-electrospray	69:95	arg1	spectrometry					111:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	7	5	theme	antitumor	1177:1185	arg1	activities					1140:1149	the biological activities	1125:1149	the biological activities (i.e. anticoagulation and antitumor action) of CCA	1125:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	5	theme	antitumor	1177:1185	arg1	action					1187:1192	antitumor action	1177:1192	antitumor action	1177:1192	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	4	6	theme	sensitivity	663:673	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	6	theme	sensitivity	663:673	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	1	7	theme	traditional	235:245	arg1	medicine					255:262	traditional Chinese medicine	235:262	traditional Chinese medicine	235:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	0	8	theme	mass	106:109	arg1	spectrometry					111:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	0	9	from	Analysis	0:7	arg1	asini					53:57	Colla corii asini	41:57	Colla corii asini	41:57	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	1	10	theme	Chinese	247:253	arg1	medicine					255:262	traditional Chinese medicine	235:262	traditional Chinese medicine	235:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	0	11	theme	trap	101:104	arg1	spectrometry					111:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	0	12	from	asini	53:57	arg1	Analysis					0:7	Analysis	0:7	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.	0:123	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	0	12	from	asini	53:57	arg1	sulfate/heparin					20:34	Heparan sulfate/heparin	12:34	Heparan sulfate/heparin from Colla corii asini	12:57	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	2	13	theme	sulfate	273:279	arg1	class					320:324	a structurally complex class	297:324	a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	297:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	2	13	theme	sulfate	273:279	arg1	/heparin					285:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	1	14	theme	medicine	255:262	arg1	asini					137:141	Colla corii asini	125:141	Colla corii asini (CCA) made from donkey-hide	125:169	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	14	theme	medicine	255:262	arg1	food					209:212	a health-care food	195:212	a health-care food	195:212	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	14	theme	medicine	255:262	arg1	ingredient					221:230	an ingredient	218:230	an ingredient of traditional Chinese medicine	218:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	4	15	theme	trap/time-of-flight	701:719	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	15	theme	trap/time-of-flight	701:719	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	7	16	theme	activities	1140:1149	arg1	understanding					1108:1120	a better understanding	1099:1120	a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA	1099:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	2	17	theme	Heparan	265:271	arg1	sulfate					273:279	Heparan sulfate	265:279	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	2	17	theme	Heparan	265:271	arg1	HS					282:283	HS	282:283	HS	282:283	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	5	18	theme	rare	840:843	arg1	disaccharides					861:873	the four rare N-unsubstituted disaccharides	831:873	the four rare N-unsubstituted disaccharides	831:873	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	4	19	theme	GAG	546:548	arg1	fractions					550:558	GAG fractions	546:558	GAG fractions containing HS/heparin	546:580	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	7	20	dep	anticoagulation	1157:1171	arg1	i.e.					1152:1155	i.e.	1152:1155	i.e.	1152:1155	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	4	21	theme	disaccharide	615:626	arg1	compositions					628:639	their disaccharide compositions	609:639	their disaccharide compositions	609:639	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	2	22	theme	activities	411:420	arg1	range					391:395	a wide range	384:395	a wide range of biological activities	384:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	6	23	theme	HS/heparin	980:989	arg1	fractions					991:999	HS/heparin fractions	980:999	HS/heparin fractions indicating chains with differing domain structures	980:1050	The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures.
31016560	2	24	theme	biological	400:409	arg1	activities					411:420	biological activities	400:420	biological activities	400:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	7	25	theme	biological	1129:1138	arg1	activities					1140:1149	the biological activities	1125:1149	the biological activities (i.e. anticoagulation and antitumor action) of CCA	1125:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	25	theme	biological	1129:1138	arg1	anticoagulation					1157:1171	anticoagulation	1157:1171	anticoagulation	1157:1171	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	25	theme	biological	1129:1138	arg1	action					1187:1192	antitumor action	1177:1192	antitumor action	1177:1192	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	26	theme	better	1101:1106	arg1	understanding					1108:1120	a better understanding	1099:1120	a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA	1099:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	0	27	theme	sulfate/heparin	20:34	arg1	Analysis					0:7	Analysis	0:7	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.	0:123	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	4	28	attach	isolated	587:594	arg2	fractions					550:558	GAG fractions	546:558	GAG fractions containing HS/heparin	546:580	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	28	attach	isolated	587:594	arg1	CCA					601:603	CCA	601:603	CCA	601:603	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	2	29	theme	wide	386:389	arg1	range					391:395	a wide range	384:395	a wide range of biological activities	384:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	0	30	theme	Heparan	12:18	arg1	sulfate/heparin					20:34	Heparan sulfate/heparin	12:34	Heparan sulfate/heparin from Colla corii asini	12:57	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	5	31	theme	significant	897:907	arg1	quantities					909:918	significant quantities	897:918	significant quantities	897:918	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	4	32	theme	high	658:661	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	32	theme	high	658:661	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	0	33	theme	Colla	41:45	arg1	asini					53:57	Colla corii asini	41:57	Colla corii asini	41:57	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	2	34	theme	complex	312:318	arg1	class					320:324	a structurally complex class	297:324	a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	297:420	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	2	34	theme	complex	312:318	arg1	/heparin					285:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin	265:292	Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.
31016560	7	35	theme	CCA	1198:1200	arg1	activities					1140:1149	the biological activities	1125:1149	the biological activities (i.e. anticoagulation and antitumor action) of CCA	1125:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	35	theme	CCA	1198:1200	arg1	anticoagulation					1157:1171	anticoagulation	1157:1171	anticoagulation	1157:1171	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	35	theme	CCA	1198:1200	arg1	action					1187:1192	antitumor action	1177:1192	antitumor action	1177:1192	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	6	36	theme	domain	1034:1039	arg1	structures					1041:1050	domain structures	1034:1050	domain structures	1034:1050	The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures.
31016560	5	37	theme	seen	808:811	arg1	disaccharides					816:828	the eight commonly seen HS disaccharides	789:828	the eight commonly seen HS disaccharides	789:828	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	7	38	theme	novel	1058:1062	arg1	information					1075:1085	This novel structural information	1053:1085	This novel structural information	1053:1085	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	1	39	theme	health-care	197:207	arg1	asini					137:141	Colla corii asini	125:141	Colla corii asini (CCA) made from donkey-hide	125:169	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	39	theme	health-care	197:207	arg1	food					209:212	a health-care food	195:212	a health-care food	195:212	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	7	40	dep	activities	1140:1149	arg1	activities					1140:1149	the biological activities	1125:1149	the biological activities (i.e. anticoagulation and antitumor action) of CCA	1125:1200	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	40	dep	activities	1140:1149	arg1	anticoagulation					1157:1171	anticoagulation	1157:1171	anticoagulation	1157:1171	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	40	dep	activities	1140:1149	arg1	action					1187:1192	antitumor action	1177:1192	antitumor action	1177:1192	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	7	41	theme	structural	1064:1073	arg1	information					1075:1085	This novel structural information	1053:1085	This novel structural information	1053:1085	This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
31016560	1	42	theme	Colla	125:129	arg1	asini					137:141	Colla corii asini	125:141	Colla corii asini (CCA) made from donkey-hide	125:169	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	42	theme	Colla	125:129	arg1	food					209:212	a health-care food	195:212	a health-care food	195:212	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	42	theme	Colla	125:129	arg1	ingredient					221:230	an ingredient	218:230	an ingredient of traditional Chinese medicine	218:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	42	theme	Colla	125:129	arg1	CCA					144:146	CCA	144:146	CCA	144:146	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	0	43	theme	corii	47:51	arg1	asini					53:57	Colla corii asini	41:57	Colla corii asini	41:57	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	1	44	used	used	187:190	arg2	food					209:212	a health-care food	195:212	a health-care food	195:212	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	44	used	used	187:190	arg2	ingredient					221:230	an ingredient	218:230	an ingredient of traditional Chinese medicine	218:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	44	used	used	187:190	arg2	CCA					144:146	CCA	144:146	CCA	144:146	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	44	used	used	187:190	arg2	asini					137:141	Colla corii asini	125:141	Colla corii asini (CCA) made from donkey-hide	125:169	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	45	theme	corii	131:135	arg1	asini					137:141	Colla corii asini	125:141	Colla corii asini (CCA) made from donkey-hide	125:169	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	45	theme	corii	131:135	arg1	food					209:212	a health-care food	195:212	a health-care food	195:212	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	45	theme	corii	131:135	arg1	ingredient					221:230	an ingredient	218:230	an ingredient of traditional Chinese medicine	218:262	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	1	45	theme	corii	131:135	arg1	CCA					144:146	CCA	144:146	CCA	144:146	Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine.
31016560	4	46	theme	mass	721:724	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	46	theme	mass	721:724	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	0	47	theme	liquid	62:67	arg1	spectrometry					111:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	liquid chromatography-electrospray ion trap mass spectrometry	62:122	Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.
31016560	4	48	contain	containing	560:569	arg2	HS/heparin					571:580	HS/heparin	571:580	HS/heparin	571:580	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	48	contain	containing	560:569	arg1	fractions					550:558	GAG fractions	546:558	GAG fractions containing HS/heparin	546:580	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	3	49	theme	possible	469:476	arg1	characteristics					489:503	their possible structural characteristics	463:503	their possible structural characteristics	463:503	However, their presence within CCA, and their possible structural characteristics, were previously unknown.
31016560	5	50	theme	N-unsubstituted	845:859	arg1	disaccharides					861:873	the four rare N-unsubstituted disaccharides	831:873	the four rare N-unsubstituted disaccharides	831:873	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	6	51	theme	disaccharide	925:936	arg1	compositions					938:949	The disaccharide compositions	921:949	The disaccharide compositions	921:949	The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures.
31016560	5	52	theme	HS	813:814	arg1	disaccharides					816:828	the eight commonly seen HS disaccharides	789:828	the eight commonly seen HS disaccharides	789:828	This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities.
31016560	3	53	theme	structural	478:487	arg1	characteristics					489:503	their possible structural characteristics	463:503	their possible structural characteristics	463:503	However, their presence within CCA, and their possible structural characteristics, were previously unknown.
31016560	4	54	theme	chromatography-ion	682:699	arg1	LC-MS-ITTOF					740:750	LC-MS-ITTOF	740:750	LC-MS-ITTOF	740:750	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
31016560	4	54	theme	chromatography-ion	682:699	arg1	spectrometry					726:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	658:737	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	658:751	In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).
30175949	11	0	theme	Waals	1571:1575	arg1	forces					1577:1582	van der Waals forces	1563:1582	van der Waals forces	1563:1582	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	1	1	theme	cell	256:259	arg1	wall					261:264	the cell wall	252:264	the cell wall	252:264	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	0	2	from	fruit	156:160	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	2	from	fruit	156:160	arg1	structure					54:62	structure	54:62	structure	54:62	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	2	from	fruit	156:160	arg1	proteins					122:129	three alpha-expansin proteins	101:129	three alpha-expansin proteins from Fragaria chiloensis fruit	101:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	11	3	theme	van	1563:1565	arg1	forces					1577:1582	van der Waals forces	1563:1582	van der Waals forces	1563:1582	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	7	4	with	module	935:940	arg1	structure					966:974	a β-sandwich structure	953:974	a β-sandwich structure	953:974	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	6	5	theme	protein	804:810	arg1	models					812:817	3D protein models	801:817	3D protein models	801:817	With the aim of elucidating their roles, 3D protein models were built using comparative modeling methodology.
30175949	1	6	theme	cell	177:180	arg1	proteins					187:194	cell wall proteins	177:194	cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit	177:327	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	1	6	theme	cell	177:180	arg1	Expansins					163:171	Expansins	163:171	Expansins	163:171	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	0	7	theme	chiloensis	145:154	arg1	fruit					156:160	Fragaria chiloensis fruit	136:160	Fragaria chiloensis fruit	136:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	12	8	theme	dynamic	1631:1637	arg1	view					1639:1642	a dynamic view	1629:1642	a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale	1629:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	5	9	theme	expression	725:734	arg1	patterns					736:743	differential but overlapping expression patterns	696:743	differential but overlapping expression patterns	696:743	Interestingly, differential but overlapping expression patterns were observed.
30175949	9	10	theme	protein-ligand	1205:1218	arg1	interactions					1220:1231	Their protein-ligand interactions	1199:1231	Their protein-ligand interactions	1199:1231	Their protein-ligand interactions were evaluated, showing favorable binding affinity energies with xyloglucan, homogalacturonan, and cellulose, cellulose being the best ligand.
30175949	4	11	theme	chiloensis	649:658	arg1	fruit					660:664	F. chiloensis fruit	646:664	F. chiloensis fruit	646:664	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	13	12	theme	H.	1771:1772	arg1	Sarma					1774:1778	Ramaswamy H. Sarma	1761:1778	Ramaswamy H. Sarma	1761:1778	Communicated by Ramaswamy H. Sarma.
30175949	7	13	theme	glycosyl	1061:1068	arg1	hydrolase					1070:1078	the family 45 glycosyl hydrolase	1047:1078	the family 45 glycosyl hydrolase	1047:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	7	14	theme	protein	1036:1042	arg1	core					1028:1031	the catalytic core	1014:1031	the catalytic core of protein of the family 45 glycosyl hydrolase	1014:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	7	15	theme	family	1051:1056	arg1	hydrolase					1070:1078	the family 45 glycosyl hydrolase	1047:1078	the family 45 glycosyl hydrolase	1047:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	11	16	theme	forces	1577:1582	arg1	contribution					1547:1558	the major contribution	1537:1558	the major contribution of van der Waals forces and non-polar interactions	1537:1609	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	9	17	theme	favorable	1257:1265	arg1	energies					1284:1291	favorable binding affinity energies	1257:1291	favorable binding affinity energies	1257:1291	Their protein-ligand interactions were evaluated, showing favorable binding affinity energies with xyloglucan, homogalacturonan, and cellulose, cellulose being the best ligand.
30175949	7	18	theme	comparable	1000:1009	arg1	domain					993:998	a catalytic domain	981:998	a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase	981:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	7	19	with	domain	993:998	arg1	structure					966:974	a β-sandwich structure	953:974	a β-sandwich structure	953:974	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	9	20	theme	affinity	1275:1282	arg1	energies					1284:1291	favorable binding affinity energies	1257:1291	favorable binding affinity energies	1257:1291	Their protein-ligand interactions were evaluated, showing favorable binding affinity energies with xyloglucan, homogalacturonan, and cellulose, cellulose being the best ligand.
30175949	7	21	theme	catalytic	983:991	arg1	domain					993:998	a catalytic domain	981:998	a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase	981:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	12	22	theme	molecular	1728:1736	arg1	scale					1738:1742	the molecular scale	1724:1742	the molecular scale	1724:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	4	23	theme	full-length	513:523	arg1	sequences					525:533	Their full-length sequences	507:533	Their full-length sequences	507:533	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	11	24	theme	major	1541:1545	arg1	contribution					1547:1558	the major contribution	1537:1558	the major contribution of van der Waals forces and non-polar interactions	1537:1609	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	0	25	theme	interaction	75:85	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	12	26	theme	putative	1694:1701	arg1	ligands					1713:1719	putative cell wall ligands	1694:1719	putative cell wall ligands at the molecular scale	1694:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	0	27	from	differences	35:45	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	27	from	differences	35:45	arg1	structure					54:62	structure	54:62	structure	54:62	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	11	28	theme	Molecular	1459:1467	arg1	analyses					1519:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	12	29	theme	wall	1708:1711	arg1	ligands					1713:1719	putative cell wall ligands	1694:1719	putative cell wall ligands at the molecular scale	1694:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	0	30	theme	Comparative	0:10	arg1	study					22:26	Comparative in silico study	0:26	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.	0:161	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	2	31	dep	Fragaria	438:445	arg1	chiloensis					447:456	chiloensis	447:456	chiloensis	447:456	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	1	32	theme	fruit	285:289	arg1	ripening					273:280	ripening	273:280	ripening	273:280	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	11	33	theme	Born-surface	1491:1502	arg1	MM-GBSA					1510:1516	MM-GBSA	1510:1516	MM-GBSA	1510:1516	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	11	33	theme	Born-surface	1491:1502	arg1	area					1504:1507	mechanics-generalized Born-surface area	1469:1507	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	2	34	theme	expansins	396:404	arg1	transcripts					406:416	specific expansins transcripts	387:416	specific expansins transcripts	387:416	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	0	35	theme	differences	35:45	arg1	study					22:26	Comparative in silico study	0:26	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.	0:161	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	11	36	theme	interactions	1598:1609	arg1	contribution					1547:1558	the major contribution	1537:1558	the major contribution of van der Waals forces and non-polar interactions	1537:1609	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	8	37	theme	central	1111:1117	arg1	part					1119:1122	the central part	1107:1122	the central part of each expansin	1107:1139	An open groove located at the central part of each expansin was described; however, the shape and size are different.
30175949	6	38	theme	modeling	848:855	arg1	methodology					857:867	comparative modeling methodology	836:867	comparative modeling methodology	836:867	With the aim of elucidating their roles, 3D protein models were built using comparative modeling methodology.
30175949	7	39	theme	cellulose	917:925	arg1	module					935:940	cellulose binding module	917:940	cellulose binding module(CBM ) with a β-sandwich structure	917:974	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	7	39	theme	cellulose	917:925	arg1	CBM					942:944	CBM	942:944	CBM	942:944	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	8	40	theme	expansin	1132:1139	arg1	part					1119:1122	the central part	1107:1122	the central part of each expansin	1107:1139	An open groove located at the central part of each expansin was described; however, the shape and size are different.
30175949	10	41	theme	small	1385:1389	arg1	differences					1391:1401	small differences	1385:1401	small differences	1385:1401	However, small differences were observed between the protein-ligand conformations.
30175949	11	42	theme	der	1567:1569	arg1	forces					1577:1582	van der Waals forces	1563:1582	van der Waals forces	1563:1582	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	4	43	theme	fruit	660:664	arg1	softening					633:641	softening	633:641	softening of F. chiloensis fruit	633:664	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	6	44	theme	3D	801:802	arg1	models					812:817	3D protein models	801:817	3D protein models	801:817	With the aim of elucidating their roles, 3D protein models were built using comparative modeling methodology.
30175949	1	45	theme	wall	182:185	arg1	proteins					187:194	cell wall proteins	177:194	cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit	177:327	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	1	45	theme	wall	182:185	arg1	Expansins					163:171	Expansins	163:171	Expansins	163:171	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	7	46	theme	hydrolase	1070:1078	arg1	protein					1036:1042	protein	1036:1042	protein of the family 45 glycosyl hydrolase	1036:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	11	47	theme	non-polar	1588:1596	arg1	interactions					1598:1609	non-polar interactions	1588:1609	non-polar interactions	1588:1609	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	12	48	theme	interaction	1647:1657	arg1	view					1639:1642	a dynamic view	1629:1642	a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale	1629:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	13	49	theme	Ramaswamy	1761:1769	arg1	Sarma					1774:1778	Ramaswamy H. Sarma	1761:1778	Ramaswamy H. Sarma	1761:1778	Communicated by Ramaswamy H. Sarma.
30175949	0	50	theme	Fragaria	136:143	arg1	fruit					156:160	Fragaria chiloensis fruit	136:160	Fragaria chiloensis fruit	136:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	1	51	theme	several	212:218	arg1	changes					241:247	changes	241:247	changes in the cell wall during ripening of fruit, which matches softening of the fruit	241:327	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	1	51	theme	several	212:218	arg1	processes					220:228	several processes	212:228	several processes	212:228	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	0	52	from	structure	54:62	arg1	fruit					156:160	Fragaria chiloensis fruit	136:160	Fragaria chiloensis fruit	136:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	52	from	structure	54:62	arg1	study					22:26	Comparative in silico study	0:26	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.	0:161	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	4	53	theme	F.	646:647	arg1	fruit					660:664	F. chiloensis fruit	646:664	F. chiloensis fruit	646:664	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	1	54	from	changes	241:247	arg1	wall					261:264	the cell wall	252:264	the cell wall	252:264	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	0	55	theme	proteins	122:129	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	55	theme	proteins	122:129	arg1	structure					54:62	structure	54:62	structure	54:62	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	12	56	from	scale	1738:1742	arg1	ligands					1713:1719	putative cell wall ligands	1694:1719	putative cell wall ligands at the molecular scale	1694:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	5	57	theme	differential	696:707	arg1	patterns					736:743	differential but overlapping expression patterns	696:743	differential but overlapping expression patterns	696:743	Interestingly, differential but overlapping expression patterns were observed.
30175949	7	58	theme	catalytic	1018:1026	arg1	core					1028:1031	the catalytic core	1014:1031	the catalytic core of protein of the family 45 glycosyl hydrolase	1014:1078	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	0	59	from	properties	87:96	arg1	fruit					156:160	Fragaria chiloensis fruit	136:160	Fragaria chiloensis fruit	136:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	59	from	properties	87:96	arg1	study					22:26	Comparative in silico study	0:26	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.	0:161	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	9	60	theme	binding	1267:1273	arg1	energies					1284:1291	favorable binding affinity energies	1257:1291	favorable binding affinity energies	1257:1291	Their protein-ligand interactions were evaluated, showing favorable binding affinity energies with xyloglucan, homogalacturonan, and cellulose, cellulose being the best ligand.
30175949	0	61	from	study	22:26	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	0	61	from	study	22:26	arg1	structure					54:62	structure	54:62	structure	54:62	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	5	62	theme	overlapping	713:723	arg1	patterns					736:743	differential but overlapping expression patterns	696:743	differential but overlapping expression patterns	696:743	Interestingly, differential but overlapping expression patterns were observed.
30175949	0	63	theme	ligand	68:73	arg1	properties					87:96	ligand interaction properties	68:96	ligand interaction properties	68:96	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	1	64	theme	fruit	323:327	arg1	softening					306:314	softening	306:314	softening of the fruit	306:327	Expansins are cell wall proteins associated with several processes, including changes in the cell wall during ripening of fruit, which matches softening of the fruit.
30175949	8	65	from	part	1119:1122	arg1	located					1096:1102	located	1096:1102	located	1096:1102	An open groove located at the central part of each expansin was described; however, the shape and size are different.
30175949	0	66	theme	alpha-expansin	107:120	arg1	proteins					122:129	three alpha-expansin proteins	101:129	three alpha-expansin proteins from Fragaria chiloensis fruit	101:160	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	10	67	theme	protein-ligand	1429:1442	arg1	conformations					1444:1456	the protein-ligand conformations	1425:1456	the protein-ligand conformations	1425:1456	However, small differences were observed between the protein-ligand conformations.
30175949	0	68	theme	in	12:13	arg1	study					22:26	Comparative in silico study	0:26	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.	0:161	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	7	69	theme	β-sandwich	955:964	arg1	structure					966:974	a β-sandwich structure	953:974	a β-sandwich structure	953:974	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	12	70	theme	cell	1703:1706	arg1	ligands					1713:1719	putative cell wall ligands	1694:1719	putative cell wall ligands at the molecular scale	1694:1742	The data provide a dynamic view of interaction between expansins and cellulose as putative cell wall ligands at the molecular scale.
30175949	2	71	theme	Fragaria	438:445	arg1	fruit					458:462	Fragaria chiloensis fruit	438:462	Fragaria chiloensis fruit	438:462	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	4	72	theme	qRT-PCR	562:568	arg1	analyses					586:593	qRT-PCR (real-time PCR) analyses	562:593	qRT-PCR (real-time PCR) analyses	562:593	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	11	73	theme	area	1504:1507	arg1	analyses					1519:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	8	74	theme	open	1084:1087	arg1	groove					1089:1094	An open groove	1081:1094	An open groove located at the central part of each expansin	1081:1139	An open groove located at the central part of each expansin was described; however, the shape and size are different.
30175949	0	75	dep	structure	54:62	arg1	the					50:52	the	50:52	the	50:52	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
30175949	4	76	theme	real-time	571:579	arg1	qRT-PCR					562:568	qRT-PCR	562:568	qRT-PCR (real-time PCR) analyses	562:593	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	4	76	theme	real-time	571:579	arg1	PCR					581:583	real-time PCR	571:583	real-time PCR	571:583	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	11	77	theme	mechanics-generalized	1469:1489	arg1	MM-GBSA					1510:1516	MM-GBSA	1510:1516	MM-GBSA	1510:1516	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	11	77	theme	mechanics-generalized	1469:1489	arg1	area					1504:1507	mechanics-generalized Born-surface area	1469:1507	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses	1459:1526	Molecular mechanics-generalized Born-surface area (MM-GBSA) analyses indicate the major contribution of van der Waals forces and non-polar interactions.
30175949	2	78	theme	transcripts	406:416	arg1	expression					373:382	expression	373:382	expression of specific expansins transcripts	373:416	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	8	79	theme	located	1096:1102	arg1	groove					1089:1094	An open groove	1081:1094	An open groove located at the central part of each expansin	1081:1139	An open groove located at the central part of each expansin was described; however, the shape and size are different.
30175949	2	80	theme	specific	387:394	arg1	transcripts					406:416	specific expansins transcripts	387:416	specific expansins transcripts	387:416	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	9	81	theme	best	1363:1366	arg1	ligand					1368:1373	the best ligand	1359:1373	the best ligand	1359:1373	Their protein-ligand interactions were evaluated, showing favorable binding affinity energies with xyloglucan, homogalacturonan, and cellulose, cellulose being the best ligand.
30175949	2	82	from	increase	361:368	arg1	expression					373:382	expression	373:382	expression of specific expansins transcripts	373:416	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	2	83	theme	fruit	458:462	arg1	softening					425:433	softening	425:433	softening of Fragaria chiloensis fruit	425:462	We have previously reported an increase in expression of specific expansins transcripts during softening of Fragaria chiloensis fruit.
30175949	4	84	theme	transcript	602:611	arg1	accumulation					613:624	their transcript accumulation	596:624	their transcript accumulation during softening of F. chiloensis fruit	596:664	Their full-length sequences were obtained, and through qRT-PCR (real-time PCR) analyses, their transcript accumulation during softening of F. chiloensis fruit was confirmed.
30175949	7	85	theme	binding	927:933	arg1	module					935:940	cellulose binding module	917:940	cellulose binding module(CBM ) with a β-sandwich structure	917:974	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	7	85	theme	binding	927:933	arg1	CBM					942:944	CBM	942:944	CBM	942:944	The models obtained were similar and displayed cellulose binding module(CBM ) with a β-sandwich structure, and a catalytic domain comparable to the catalytic core of protein of the family 45 glycosyl hydrolase.
30175949	6	86	theme	comparative	836:846	arg1	methodology					857:867	comparative modeling methodology	836:867	comparative modeling methodology	836:867	With the aim of elucidating their roles, 3D protein models were built using comparative modeling methodology.
30175949	0	87	dep	in	12:13	arg1	silico					15:20	silico	15:20	silico	15:20	Comparative in silico study of the differences in the structure and ligand interaction properties of three alpha-expansin proteins from Fragaria chiloensis fruit.
31497643	0	0	theme	multispectral	64:76	arg1	imaging					78:84	2D to 3D x-ray Raman multispectral imaging	43:84	2D to 3D x-ray Raman multispectral imaging	43:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	5	1	theme	organic	1050:1056	arg1	specimens					1058:1066	organic specimens	1050:1066	organic specimens	1050:1066	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	1	2	theme	3D	124:125	arg1	problem					196:202	an unsolved problem	184:202	an unsolved problem in paleontology and cultural heritage	184:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	2	theme	3D	124:125	arg1	imaging					127:133	The in situ two-dimensional (2D) and 3D imaging	87:133	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils	87:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	2	3	theme	53-million-year-old	496:514	arg1	amber					516:520	53-million-year-old amber	496:520	53-million-year-old amber	496:520	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	1	4	theme	paleontology	207:218	arg1	heritage					233:240	paleontology and cultural heritage	207:240	paleontology and cultural heritage	207:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	0	5	theme	Raman	58:62	arg1	imaging					78:84	2D to 3D x-ray Raman multispectral imaging	43:84	2D to 3D x-ray Raman multispectral imaging	43:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	0	6	from	speciation	7:16	arg1	fossils					29:35	organic fossils	21:35	organic fossils using 2D to 3D x-ray Raman multispectral imaging	21:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	4	7	theme	chemical	787:794	arg1	signature					796:804	an exceptionally well preserved remaining chemical signature	745:804	an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion	745:886	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	3	8	theme	fossil	555:560	arg1	imaging					534:540	The 2D XRS imaging	523:540	The 2D XRS imaging of the plant fossil	523:560	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	5	9	theme	in	930:931	arg1	imaging					958:964	in situ chemical speciation imaging	930:964	in situ chemical speciation imaging of fossilized organic materials	930:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	1	10	theme	cultural	224:231	arg1	heritage					233:240	paleontology and cultural heritage	207:240	paleontology and cultural heritage	207:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	3	11	theme	micrometric	610:620	arg1	"					630:630	micrometric "pockets"	610:630	micrometric "pockets" of preservation	610:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	4	12	theme	typical	806:812	arg1	signature					796:804	an exceptionally well preserved remaining chemical signature	745:804	an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion	745:886	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	4	13	theme	3D	699:700	arg1	imaging					706:712	The 3D XRS imaging	695:712	The 3D XRS imaging of the insect cuticle	695:734	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	2	14	theme	2D	328:329	arg1	images					338:343	2D and 3D images	328:343	images	338:343	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	5	15	theme	specimens	1058:1066	arg1	understanding					1033:1045	our understanding	1029:1045	our understanding of organic specimens	1029:1066	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	0	16	dep	imaging	78:84	arg1	to					46:47	to	46:47	to	46:47	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	3	17	with	composition	593:603	arg1	"					630:630	micrometric "pockets"	610:630	micrometric "pockets" of preservation	610:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	3	18	theme	preservation	635:646	arg1	"					630:630	micrometric "pockets"	610:630	micrometric "pockets" of preservation	610:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	4	19	theme	remaining	777:785	arg1	signature					796:804	an exceptionally well preserved remaining chemical signature	745:804	an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion	745:886	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	1	20	theme	chemical	142:149	arg1	speciation					151:160	the chemical speciation	138:160	the chemical speciation of organic fossils	138:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	0	21	theme	Carbon	0:5	arg1	speciation					7:16	Carbon speciation	0:16	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.	0:85	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	4	22	theme	preserved	767:775	arg1	signature					796:804	an exceptionally well preserved remaining chemical signature	745:804	an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion	745:886	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	2	23	theme	Raman	262:266	arg1	scattering					268:277	x-ray Raman scattering	256:277	x-ray Raman scattering	256:277	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	1	24	theme	speciation	151:160	arg1	problem					196:202	an unsolved problem	184:202	an unsolved problem in paleontology and cultural heritage	184:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	24	theme	speciation	151:160	arg1	imaging					127:133	The in situ two-dimensional (2D) and 3D imaging	87:133	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils	87:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	0	25	theme	organic	21:27	arg1	fossils					29:35	organic fossils	21:35	organic fossils using 2D to 3D x-ray Raman multispectral imaging	21:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	3	26	theme	geological	675:684	arg1	history					686:692	its geological history	671:692	its geological history	671:692	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	2	27	theme	ancient	468:474	arg1	insect					476:481	an ancient insect	465:481	an ancient insect	465:481	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	2	28	theme	x-ray	256:260	arg1	scattering					268:277	x-ray Raman scattering	256:277	x-ray Raman scattering	256:277	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	5	29	theme	speciation	947:956	arg1	imaging					958:964	in situ chemical speciation imaging	930:964	in situ chemical speciation imaging of fossilized organic materials	930:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	2	30	theme	chemistry	359:367	arg1	images					338:343	2D and 3D images	328:343	images	338:343	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	1	31	theme	in	91:92	arg1	problem					196:202	an unsolved problem	184:202	an unsolved problem in paleontology and cultural heritage	184:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	31	theme	in	91:92	arg1	imaging					127:133	The in situ two-dimensional (2D) and 3D imaging	87:133	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils	87:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	32	theme	organic	165:171	arg1	fossils					173:179	organic fossils	165:179	organic fossils	165:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	4	33	theme	hollowed-out	865:876	arg1	inclusion					878:886	a largely hollowed-out inclusion	855:886	a largely hollowed-out inclusion	855:886	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	2	34	theme	3D	335:336	arg1	images					338:343	2D and 3D images	328:343	images	338:343	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	4	35	theme	cuticle	728:734	arg1	imaging					706:712	The 3D XRS imaging	695:712	The 3D XRS imaging of the insect cuticle	695:734	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	1	36	theme	fossils	173:179	arg1	speciation					151:160	the chemical speciation	138:160	the chemical speciation of organic fossils	138:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	2	37	theme	preserved	390:398	arg1	plant					420:424	a fossil plant	411:424	a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber	411:520	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	2	37	theme	preserved	390:398	arg1	specimens					400:408	two exceptionally preserved specimens	372:408	two exceptionally preserved specimens	372:408	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	3	38	theme	plant	549:553	arg1	fossil					555:560	the plant fossil	545:560	the plant fossil	545:560	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	2	39	theme	carbon	352:357	arg1	chemistry					359:367	the carbon chemistry	348:367	the carbon chemistry	348:367	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	5	40	dep	in	930:931	arg1	situ					933:936	situ	933:936	situ	933:936	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	5	41	theme	new	909:911	arg1	perspectives					913:924	up new perspectives	906:924	up new perspectives for in situ chemical speciation imaging of fossilized organic materials	906:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	1	42	from	problem	196:202	arg1	heritage					233:240	paleontology and cultural heritage	207:240	paleontology and cultural heritage	207:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	2	43	theme	fossil	413:418	arg1	plant					420:424	a fossil plant	411:424	a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber	411:520	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	2	43	theme	fossil	413:418	arg1	specimens					400:408	two exceptionally preserved specimens	372:408	two exceptionally preserved specimens	372:408	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	2	44	from	K-edge	313:318	arg1	imaging					291:297	x-ray Raman scattering (XRS)-based imaging	256:297	x-ray Raman scattering (XRS)-based imaging at the carbon K-edge	256:318	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	2	45	theme	-based	284:289	arg1	imaging					291:297	x-ray Raman scattering (XRS)-based imaging	256:297	x-ray Raman scattering (XRS)-based imaging at the carbon K-edge	256:318	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	0	46	theme	2D	43:44	arg1	imaging					78:84	2D to 3D x-ray Raman multispectral imaging	43:84	2D to 3D x-ray Raman multispectral imaging	43:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	4	47	theme	polysaccharides	817:831	arg1	typical					806:812	typical	806:812	typical	806:812	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	5	48	theme	organic	980:986	arg1	materials					988:996	fossilized organic materials	969:996	fossilized organic materials	969:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	5	49	theme	fossilized	969:978	arg1	materials					988:996	fossilized organic materials	969:996	fossilized organic materials	969:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	1	50	theme	two-dimensional	99:113	arg1	problem					196:202	an unsolved problem	184:202	an unsolved problem in paleontology and cultural heritage	184:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	50	theme	two-dimensional	99:113	arg1	imaging					127:133	The in situ two-dimensional (2D) and 3D imaging	87:133	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils	87:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	4	51	theme	insect	721:726	arg1	cuticle					728:734	the insect cuticle	717:734	the insect cuticle	717:734	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	5	52	theme	materials	988:996	arg1	imaging					958:964	in situ chemical speciation imaging	930:964	in situ chemical speciation imaging of fossilized organic materials	930:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	3	53	theme	2D	527:528	arg1	imaging					534:540	The 2D XRS imaging	523:540	The 2D XRS imaging of the plant fossil	523:560	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	2	54	dep	-based	284:289	arg1	scattering					268:277	x-ray Raman scattering	256:277	x-ray Raman scattering	256:277	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	1	55	dep	two-dimensional	99:113	arg1	2D					116:117	2D	116:117	2D	116:117	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	3	56	theme	homogeneous	572:582	arg1	composition					593:603	a homogeneous chemical composition	570:603	a homogeneous chemical composition with micrometric "pockets" of preservation	570:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	4	57	theme	XRS	702:704	arg1	imaging					706:712	The 3D XRS imaging	695:712	The 3D XRS imaging of the insect cuticle	695:734	The 3D XRS imaging of the insect cuticle displays an exceptionally well preserved remaining chemical signature typical of polysaccharides such as chitin around a largely hollowed-out inclusion.
31497643	1	58	theme	unsolved	187:194	arg1	problem					196:202	an unsolved problem	184:202	an unsolved problem in paleontology and cultural heritage	184:240	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	1	58	theme	unsolved	187:194	arg1	imaging					127:133	The in situ two-dimensional (2D) and 3D imaging	87:133	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils	87:179	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
31497643	0	59	theme	x-ray	52:56	arg1	imaging					78:84	2D to 3D x-ray Raman multispectral imaging	43:84	2D to 3D x-ray Raman multispectral imaging	43:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	3	60	theme	chemical	584:591	arg1	composition					593:603	a homogeneous chemical composition	570:603	a homogeneous chemical composition with micrometric "pockets" of preservation	570:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	3	61	theme	XRS	530:532	arg1	imaging					534:540	The 2D XRS imaging	523:540	The 2D XRS imaging of the plant fossil	523:560	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	2	62	theme	carbon	306:311	arg1	K-edge					313:318	the carbon K-edge	302:318	the carbon K-edge	302:318	Here, we use x-ray Raman scattering (XRS)-based imaging at the carbon K-edge to form 2D and 3D images of the carbon chemistry in two exceptionally preserved specimens, a fossil plant dating back from the Carboniferous and an ancient insect entrapped in 53-million-year-old amber.
31497643	0	63	theme	3D	49:50	arg1	imaging					78:84	2D to 3D x-ray Raman multispectral imaging	43:84	2D to 3D x-ray Raman multispectral imaging	43:84	Carbon speciation in organic fossils using 2D to 3D x-ray Raman multispectral imaging.
31497643	3	64	theme	pockets	623:629	arg1	"					630:630	micrometric "pockets"	610:630	micrometric "pockets" of preservation	610:646	The 2D XRS imaging of the plant fossil reveals a homogeneous chemical composition with micrometric "pockets" of preservation, likely inherited from its geological history.
31497643	5	65	theme	chemical	938:945	arg1	imaging					958:964	in situ chemical speciation imaging	930:964	in situ chemical speciation imaging of fossilized organic materials	930:996	Our results open up new perspectives for in situ chemical speciation imaging of fossilized organic materials, with the potential to enhance our understanding of organic specimens and their paleobiology.
31497643	1	66	dep	in	91:92	arg1	situ					94:97	situ	94:97	situ	94:97	The in situ two-dimensional (2D) and 3D imaging of the chemical speciation of organic fossils is an unsolved problem in paleontology and cultural heritage.
30296293	3	0	theme	qualitative	310:320	arg1	analysis					322:329	a qualitative analysis	308:329	a qualitative analysis of LJP	308:336	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	6	1	theme	cell	935:938	arg1	activity					940:947	natural killer (NK) cell activity	915:947	natural killer (NK) cell activity	915:947	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	1	2	theme	herbal	168:173	arg1	japonica					138:145	Lonicera japonica	129:145	Lonicera japonica	129:145	Lonicera japonica is a typical Chinese herbal medicine.
30296293	1	2	theme	herbal	168:173	arg1	medicine					175:182	a typical Chinese herbal medicine	150:182	a typical Chinese herbal medicine	150:182	Lonicera japonica is a typical Chinese herbal medicine.
30296293	0	3	theme	immunosuppressed	78:93	arg1	mice					95:98	immunosuppressed mice	78:98	immunosuppressed mice	78:98	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	0	4	from	activity	17:24	arg1	mice					95:98	immunosuppressed mice	78:98	immunosuppressed mice	78:98	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	0	4	from	activity	17:24	arg1	Lonicera					57:64	Lonicera	57:64	Lonicera	57:64	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	7	5	theme	CTX-treated	1105:1115	arg1	mice					1117:1120	the CTX-treated mice	1101:1120	the CTX-treated mice	1101:1120	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	4	6	theme	LJP	591:593	arg1	function					579:586	the immunomodulatory function	558:586	the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models	558:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30296293	6	7	theme	organ	839:843	arg1	index					845:849	the organ index	835:849	the organ index	835:849	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	0	8	from	Lonicera	57:64	arg1	polysaccharide					37:50	a novel polysaccharide	29:50	a novel polysaccharide from Lonicera japonica	29:73	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	0	8	from	Lonicera	57:64	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice	0:98	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	7	9	dep	cytokines	1013:1021	arg1	Interferon-γ					1077:1088	Interferon-γ	1077:1088	Interferon-γ (IFN-γ)	1077:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	cytokines					1013:1021	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	interleukin					1023:1033	interleukin	1023:1033	interleukin (IL-2)	1023:1040	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	IFN-γ					1091:1095	IFN-γ	1091:1095	IFN-γ	1091:1095	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	factor					1058:1063	tumor necrosis factor	1043:1063	tumor necrosis factor (TNF-α)	1043:1071	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	IL-2					1036:1039	IL-2	1036:1039	IL-2	1036:1039	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	9	dep	cytokines	1013:1021	arg1	TNF-α					1066:1070	TNF-α	1066:1070	TNF-α	1066:1070	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	3	10	theme	liquid	500:505	arg1	HPLC					523:526	HPLC	523:526	HPLC	523:526	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	10	theme	liquid	500:505	arg1	chromatography					507:520	the pre-column derivatization high performance liquid chromatography	453:520	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	4	11	theme	mouse	646:650	arg1	models					652:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30296293	6	12	from	index	845:849	arg1	mice					964:967	CTX-treated mice	952:967	CTX-treated mice	952:967	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	6	13	theme	natural	915:921	arg1	killer					923:928	natural killer	915:928	natural killer (NK) cell activity	915:947	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	6	13	theme	natural	915:921	arg1	NK					931:932	NK	931:932	NK	931:932	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	4	14	from	function	579:586	arg1	models					652:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30296293	3	15	theme	LJP	443:445	arg1	composition					428:438	the monosaccharide composition	409:438	the monosaccharide composition of LJP	409:445	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	8	16	theme	T-lymphocytes	1161:1173	arg1	subsets					1175:1181	the T-lymphocytes subsets	1157:1181	the T-lymphocytes subsets of spleen	1157:1191	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	6	17	theme	killer	923:928	arg1	activity					940:947	natural killer (NK) cell activity	915:947	natural killer (NK) cell activity	915:947	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	2	18	from	Lonicera	249:256	arg1	polysaccharides					228:242	polysaccharides	228:242	polysaccharides from Lonicera japonica (LJP)	228:271	We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP).
30296293	3	19	theme	monosaccharide	413:426	arg1	composition					428:438	the monosaccharide composition	409:438	the monosaccharide composition of LJP	409:445	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	0	20	theme	Immunomodulatory	0:15	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice	0:98	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	4	21	theme	immunomodulatory	562:577	arg1	function					579:586	the immunomodulatory function	558:586	the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models	558:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30296293	9	22	used	used	1321:1324	arg2	agent					1358:1362	a potential immunomodulatory agent	1329:1362	a potential immunomodulatory agent	1329:1362	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	9	22	used	used	1321:1324	arg2	LJP					1308:1310	LJP	1308:1310	LJP	1308:1310	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	3	23	theme	Infrared	366:373	arg1	FT-IR					389:393	FT-IR	389:393	FT-IR	389:393	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	23	theme	Infrared	366:373	arg1	Spectrometer					375:386	Infrared Spectrometer	366:386	Infrared Spectrometer (FT-IR)	366:394	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	9	24	theme	potential	1331:1339	arg1	LJP					1308:1310	LJP	1308:1310	LJP	1308:1310	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	9	24	theme	potential	1331:1339	arg1	agent					1358:1362	a potential immunomodulatory agent	1329:1362	a potential immunomodulatory agent	1329:1362	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	3	25	theme	pre-column	457:466	arg1	HPLC					523:526	HPLC	523:526	HPLC	523:526	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	25	theme	pre-column	457:466	arg1	chromatography					507:520	the pre-column derivatization high performance liquid chromatography	453:520	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	6	26	from	phagocytosis	897:908	arg1	mice					964:967	CTX-treated mice	952:967	CTX-treated mice	952:967	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	9	27	theme	immunomodulatory	1341:1356	arg1	LJP					1308:1310	LJP	1308:1310	LJP	1308:1310	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	9	27	theme	immunomodulatory	1341:1356	arg1	agent					1358:1362	a potential immunomodulatory agent	1329:1362	a potential immunomodulatory agent	1329:1362	Therefore, LJP could be used as a potential immunomodulatory agent.
30296293	5	28	theme	typical	725:731	arg1	polysaccharides					733:747	typical polysaccharides	725:747	typical polysaccharides consisting of 6 types of monosaccharides	725:788	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	3	29	theme	derivatization	468:481	arg1	HPLC					523:526	HPLC	523:526	HPLC	523:526	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	29	theme	derivatization	468:481	arg1	chromatography					507:520	the pre-column derivatization high performance liquid chromatography	453:520	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	0	30	theme	polysaccharide	37:50	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice	0:98	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	5	31	theme	polysaccharides	733:747	arg1	absorption					711:720	the characteristic absorption	692:720	the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides	692:788	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	0	32	dep	Lonicera	57:64	arg1	japonica					66:73	Lonicera japonica	57:73	Lonicera japonica	57:73	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	0	33	theme	novel	31:35	arg1	polysaccharide					37:50	a novel polysaccharide	29:50	a novel polysaccharide from Lonicera japonica	29:73	Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide.
30296293	8	34	theme	spleen	1186:1191	arg1	subsets					1175:1181	the T-lymphocytes subsets	1157:1181	the T-lymphocytes subsets of spleen	1157:1191	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	6	35	theme	macrophage	886:895	arg1	phagocytosis					897:908	macrophage phagocytosis	886:908	macrophage phagocytosis	886:908	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	2	36	dep	Lonicera	249:256	arg1	japonica					258:265	Lonicera japonica	249:265	Lonicera japonica (LJP)	249:271	We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP).
30296293	6	37	from	proliferation	871:883	arg1	mice					964:967	CTX-treated mice	952:967	CTX-treated mice	952:967	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	7	38	theme	tumor	1043:1047	arg1	TNF-α					1066:1070	TNF-α	1066:1070	TNF-α	1066:1070	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	38	theme	tumor	1043:1047	arg1	cytokines					1013:1021	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	38	theme	tumor	1043:1047	arg1	factor					1058:1063	tumor necrosis factor	1043:1063	tumor necrosis factor (TNF-α)	1043:1071	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	5	39	theme	characteristic	696:709	arg1	absorption					711:720	the characteristic absorption	692:720	the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides	692:788	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	1	40	theme	Lonicera	129:136	arg1	japonica					138:145	Lonicera japonica	129:145	Lonicera japonica	129:145	Lonicera japonica is a typical Chinese herbal medicine.
30296293	1	40	theme	Lonicera	129:136	arg1	medicine					175:182	a typical Chinese herbal medicine	150:182	a typical Chinese herbal medicine	150:182	Lonicera japonica is a typical Chinese herbal medicine.
30296293	6	41	theme	CTX-treated	952:962	arg1	mice					964:967	CTX-treated mice	952:967	CTX-treated mice	952:967	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	7	42	theme	necrosis	1049:1056	arg1	TNF-α					1066:1070	TNF-α	1066:1070	TNF-α	1066:1070	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	42	theme	necrosis	1049:1056	arg1	cytokines					1013:1021	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	42	theme	necrosis	1049:1056	arg1	factor					1058:1063	tumor necrosis factor	1043:1063	tumor necrosis factor (TNF-α)	1043:1071	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	43	theme	cytokines	1013:1021	arg1	levels					997:1002	the levels	993:1002	the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice	993:1120	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	3	44	theme	chromatography	507:520	arg1	method					529:534	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	5	45	contain	had	688:690	arg2	absorption					711:720	the characteristic absorption	692:720	the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides	692:788	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	5	45	contain	had	688:690	arg1	LJP					684:686	LJP	684:686	LJP	684:686	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	3	46	dep	Fourier	348:354	arg1	Transform					356:364	Transform	356:364	Transform Infrared Spectrometer (FT-IR)	356:394	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	6	47	theme	lymphocyte	860:869	arg1	proliferation					871:883	splenic lymphocyte proliferation	852:883	splenic lymphocyte proliferation	852:883	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	8	48	theme	immunosuppressed	1249:1264	arg1	mice					1266:1269	immunosuppressed mice	1249:1269	immunosuppressed mice from another perspective	1249:1294	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	3	49	theme	high	483:486	arg1	HPLC					523:526	HPLC	523:526	HPLC	523:526	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	49	theme	high	483:486	arg1	chromatography					507:520	the pre-column derivatization high performance liquid chromatography	453:520	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	6	50	theme	splenic	852:858	arg1	proliferation					871:883	splenic lymphocyte proliferation	852:883	splenic lymphocyte proliferation	852:883	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	8	51	from	perspective	1284:1294	arg1	mice					1266:1269	immunosuppressed mice	1249:1269	immunosuppressed mice from another perspective	1249:1294	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	3	52	theme	performance	488:498	arg1	HPLC					523:526	HPLC	523:526	HPLC	523:526	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	3	52	theme	performance	488:498	arg1	chromatography					507:520	the pre-column derivatization high performance liquid chromatography	453:520	the pre-column derivatization high performance liquid chromatography (HPLC) method	453:534	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	8	53	theme	LJP-induced	1208:1218	arg1	activity					1237:1244	LJP-induced immunomodulatory activity	1208:1244	LJP-induced immunomodulatory activity	1208:1244	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	5	54	theme	monosaccharides	774:788	arg1	types					765:769	6 types	763:769	6 types of monosaccharides	763:788	The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides.
30296293	7	55	theme	serum	1007:1011	arg1	interleukin					1023:1033	interleukin	1023:1033	interleukin (IL-2)	1023:1040	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	55	theme	serum	1007:1011	arg1	factor					1058:1063	tumor necrosis factor	1043:1063	tumor necrosis factor (TNF-α)	1043:1071	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	55	theme	serum	1007:1011	arg1	cytokines					1013:1021	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ)	1007:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	7	55	theme	serum	1007:1011	arg1	Interferon-γ					1077:1088	Interferon-γ	1077:1088	Interferon-γ (IFN-γ)	1077:1096	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	1	56	theme	typical	152:158	arg1	japonica					138:145	Lonicera japonica	129:145	Lonicera japonica	129:145	Lonicera japonica is a typical Chinese herbal medicine.
30296293	1	56	theme	typical	152:158	arg1	medicine					175:182	a typical Chinese herbal medicine	150:182	a typical Chinese herbal medicine	150:182	Lonicera japonica is a typical Chinese herbal medicine.
30296293	7	57	from	levels	997:1002	arg1	mice					1117:1120	the CTX-treated mice	1101:1120	the CTX-treated mice	1101:1120	LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice.
30296293	4	58	theme	immunosuppressed	629:644	arg1	models					652:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30296293	3	59	theme	LJP	334:336	arg1	analysis					322:329	a qualitative analysis	308:329	a qualitative analysis of LJP	308:336	In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method.
30296293	8	60	theme	immunomodulatory	1220:1235	arg1	activity					1237:1244	LJP-induced immunomodulatory activity	1208:1244	LJP-induced immunomodulatory activity	1208:1244	Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective.
30296293	1	61	theme	Chinese	160:166	arg1	japonica					138:145	Lonicera japonica	129:145	Lonicera japonica	129:145	Lonicera japonica is a typical Chinese herbal medicine.
30296293	1	61	theme	Chinese	160:166	arg1	medicine					175:182	a typical Chinese herbal medicine	150:182	a typical Chinese herbal medicine	150:182	Lonicera japonica is a typical Chinese herbal medicine.
30296293	6	62	from	activity	940:947	arg1	mice					964:967	CTX-treated mice	952:967	CTX-treated mice	952:967	In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice.
30296293	4	63	theme	-induced	620:627	arg1	models					652:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	cyclophosphamide (CTX)-induced immunosuppressed mouse models	598:657	We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models.
30623807	1	0	theme	shearing	144:151	arg1	action					153:158	hydraulic shearing action	134:158	hydraulic shearing action	134:158	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	7	1	theme	inner	1469:1473	arg1	EPS					1489:1491	inner tightly bound EPS	1469:1491	inner tightly bound EPS	1469:1491	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	7	2	dep	fragment	1379:1386	arg1	flocs					1388:1392	flocs	1388:1392	flocs	1388:1392	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	7	3	theme	surface	1425:1431	arg1	erosion					1433:1439	surface erosion	1425:1439	surface erosion	1425:1439	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	7	4	theme	bound	1483:1487	arg1	EPS					1489:1491	inner tightly bound EPS	1469:1491	inner tightly bound EPS	1469:1491	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	4	5	theme	gradient	875:882	arg1	product					851:857	the product	847:857	the product of the velocity gradient and time	847:891	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	1	6	theme	action	153:158	arg1	effects					123:129	the effects	119:129	the effects of hydraulic shearing action on activated sludge floc aggregation	119:195	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	3	7	theme	breakage	713:720	arg1	model					722:726	the breakage model	709:726	the breakage model	709:726	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	8	theme	floc	567:570	arg1	size					572:575	floc size	567:575	floc size	567:575	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	1	9	theme	different	258:266	arg1	strengths					274:282	different shear strengths	258:282	different shear strengths	258:282	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	5	10	theme	surface	1123:1129	arg1	erosion					1131:1137	surface erosion	1123:1137	surface erosion	1123:1137	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	4	11	theme	sludge	787:792	arg1	flocs					794:798	activated sludge flocs	777:798	activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	777:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	3	12	theme	polymer	541:547	arg1	substances					549:558	extracellular polymer substances	527:558	extracellular polymer substances (EPS)	527:564	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	12	theme	polymer	541:547	arg1	EPS					561:563	EPS	561:563	EPS	561:563	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	1	13	theme	shear	268:272	arg1	strengths					274:282	different shear strengths	258:282	different shear strengths	258:282	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	2	14	theme	particle	394:401	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	2	15	theme	analyser	408:415	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	3	16	theme	re-growth	732:740	arg1	mechanism					742:750	re-growth mechanism	732:750	re-growth mechanism	732:750	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	6	17	theme	bound	1260:1264	arg1	EPS					1266:1268	tightly bound EPS	1252:1268	tightly bound EPS	1252:1268	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	6	18	from	EPS	1206:1208	arg1	sludge					1213:1218	sludge	1213:1218	sludge	1213:1218	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	1	19	theme	activated	163:171	arg1	aggregation					185:195	activated sludge floc aggregation	163:195	activated sludge floc aggregation	163:195	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	0	20	theme	hydraulic	8:16	arg1	strength					24:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength and mechanism of activated sludge floc re-growth after breakage.
30623807	4	21	theme	velocity	866:873	arg1	gradient					875:882	the velocity gradient	862:882	the velocity gradient	862:882	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	1	22	from	effects	123:129	arg1	aggregation					185:195	activated sludge floc aggregation	163:195	activated sludge floc aggregation	163:195	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	1	23	theme	sludge	173:178	arg1	aggregation					185:195	activated sludge floc aggregation	163:195	activated sludge floc aggregation	163:195	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	0	24	theme	Optimal	0:6	arg1	strength					24:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength and mechanism of activated sludge floc re-growth after breakage.
30623807	5	25	attach	released	987:994	arg2	substances					976:985	The substances	972:985	The substances released from sludge	972:1006	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	5	25	attach	released	987:994	arg1	sludge					1001:1006	sludge	1001:1006	sludge	1001:1006	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	7	26	theme	re-growth	1512:1520	arg1	performance					1522:1532	re-growth performance	1512:1532	re-growth performance	1512:1532	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	7	27	theme	shear	1360:1364	arg1	strength					1366:1373	moderate hydraulic shear strength	1341:1373	moderate hydraulic shear strength	1341:1373	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	1	28	theme	floc	180:183	arg1	aggregation					185:195	activated sludge floc aggregation	163:195	activated sludge floc aggregation	163:195	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	4	29	theme	strength	828:835	arg1	value					840:844	a hydraulic shear strength GT value	810:844	a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	810:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	5	30	theme	shear	1023:1027	arg1	strength					1029:1036	shear strength	1023:1036	shear strength	1023:1036	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	4	31	theme	activated	777:785	arg1	flocs					794:798	activated sludge flocs	777:798	activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	777:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	7	32	theme	activated	1312:1320	arg1	flocs					1329:1333	activated sludge flocs	1312:1333	activated sludge flocs	1312:1333	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	0	33	theme	shear	18:22	arg1	strength					24:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength	0:31	Optimal hydraulic shear strength and mechanism of activated sludge floc re-growth after breakage.
30623807	4	34	theme	re-growth	923:931	arg1	performance					933:943	enhanced re-growth performance	914:943	enhanced re-growth performance	914:943	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	2	35	theme	size	364:367	arg1	variations					369:378	the floc size variations	355:378	the floc size variations using a laser particle size analyser on-line monitoring technique	355:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	6	36	from	spectrum	1180:1187	arg1	sludge					1213:1218	sludge	1213:1218	sludge	1213:1218	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	1	37	theme	floc	198:201	arg1	aggregation					203:213	floc aggregation	198:213	floc aggregation	198:213	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	2	38	theme	laser	388:392	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	2	39	theme	monitoring	425:434	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	7	40	theme	flocs	1329:1333	arg1	breakage					1300:1307	the breakage	1296:1307	the breakage of activated sludge flocs under moderate hydraulic shear strength	1296:1373	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	4	41	theme	GT	837:838	arg1	value					840:844	a hydraulic shear strength GT value	810:844	a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	810:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	1	42	theme	aggregation	203:213	arg1	performances					239:250	floc aggregation, breakage and re-growth performances	198:250	floc aggregation, breakage and re-growth performances under different shear strengths	198:282	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	4	43	theme	56,280	897:902	arg1	value					840:844	a hydraulic shear strength GT value	810:844	a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	810:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	7	44	theme	moderate	1341:1348	arg1	strength					1366:1373	moderate hydraulic shear strength	1341:1373	moderate hydraulic shear strength	1341:1373	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	2	45	theme	floc	359:362	arg1	variations					369:378	the floc size variations	355:378	the floc size variations using a laser particle size analyser on-line monitoring technique	355:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	7	46	theme	EPS	1489:1491	arg1	exposure					1457:1464	the exposure	1453:1464	the exposure of inner tightly bound EPS	1453:1491	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	6	47	theme	bound	1200:1204	arg1	EPS					1206:1208	loosely bound EPS	1192:1208	loosely bound EPS in sludge	1192:1218	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	7	48	theme	sludge	1322:1327	arg1	flocs					1329:1333	activated sludge flocs	1312:1333	activated sludge flocs	1312:1333	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	5	49	theme	floc	1059:1062	arg1	mode					1073:1076	the floc breakage mode	1055:1076	the floc breakage mode	1055:1076	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	3	50	theme	surface	605:611	arg1	erosion					613:619	surface erosion	605:619	surface erosion	605:619	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	51	theme	substances	549:558	arg1	structures					634:643	chemical structures	625:643	chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism	625:750	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	51	theme	substances	549:558	arg1	size					572:575	floc size	567:575	floc size	567:575	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	51	theme	substances	549:558	arg1	composition					512:522	the composition	508:522	the composition of extracellular polymer substances (EPS)	508:564	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	51	theme	substances	549:558	arg1	substances					578:587	substances	578:587	substances released due to surface erosion	578:619	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	52	theme	extracellular	527:539	arg1	substances					549:558	extracellular polymer substances	527:558	extracellular polymer substances (EPS)	527:564	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	52	theme	extracellular	527:539	arg1	EPS					561:563	EPS	561:563	EPS	561:563	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	7	53	theme	hydraulic	1350:1358	arg1	strength					1366:1373	moderate hydraulic shear strength	1341:1373	moderate hydraulic shear strength	1341:1373	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	4	54	theme	enhanced	914:921	arg1	performance					933:943	enhanced re-growth performance	914:943	enhanced re-growth performance	914:943	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	5	55	theme	breakage	1064:1071	arg1	mode					1073:1076	the floc breakage mode	1055:1076	the floc breakage mode	1055:1076	The substances released from sludge increased with shear strength, indicating that the floc breakage mode gradually transitioned from fragmentation to surface erosion.
30623807	4	56	theme	hydraulic	812:820	arg1	value					840:844	a hydraulic shear strength GT value	810:844	a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	810:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	7	57	theme	smaller	1399:1405	arg1	particles					1407:1415	smaller particles	1399:1415	smaller particles without surface erosion	1399:1439	The results suggest that the breakage of activated sludge flocs under moderate hydraulic shear strength can fragment flocs into smaller particles without surface erosion and promote the exposure of inner tightly bound EPS, thereby improving re-growth performance.
30623807	3	58	dep	transform	659:667	arg1	infrared					669:676	infrared	669:676	transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism	659:750	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	3	59	dep	Fourier	651:657	arg1	transform					659:667	transform	659:667	transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism	659:750	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	6	60	theme	EPS	1206:1208	arg1	similar					1233:1239	similar	1233:1239	similar	1233:1239	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	6	60	theme	EPS	1206:1208	arg1	spectrum					1180:1187	the spectrum	1176:1187	the spectrum of loosely bound EPS in sludge	1176:1218	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	4	61	theme	time	888:891	arg1	product					851:857	the product	847:857	the product of the velocity gradient and time	847:891	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	1	62	theme	breakage	216:223	arg1	performances					239:250	floc aggregation, breakage and re-growth performances	198:250	floc aggregation, breakage and re-growth performances under different shear strengths	198:282	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	0	63	theme	floc	67:70	arg1	re-growth					72:80	sludge floc re-growth	60:80	sludge floc re-growth	60:80	Optimal hydraulic shear strength and mechanism of activated sludge floc re-growth after breakage.
30623807	2	64	theme	on-line	417:423	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	3	65	theme	chemical	625:632	arg1	structures					634:643	chemical structures	625:643	chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism	625:750	The flocs after breakage were characterised by investigating the composition of extracellular polymer substances (EPS), floc size, substances released due to surface erosion and chemical structures using Fourier transform infrared spectroscopy (FTIR) to clarify the breakage model and re-growth mechanism.
30623807	2	66	theme	size	403:406	arg1	technique					436:444	a laser particle size analyser on-line monitoring technique	386:444	a laser particle size analyser on-line monitoring technique	386:444	Performances were evaluated by measuring the floc size variations using a laser particle size analyser on-line monitoring technique.
30623807	4	67	dep	value	840:844	arg1	product					851:857	the product	847:857	the product of the velocity gradient and time	847:891	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	0	68	theme	sludge	60:65	arg1	re-growth					72:80	sludge floc re-growth	60:80	sludge floc re-growth	60:80	Optimal hydraulic shear strength and mechanism of activated sludge floc re-growth after breakage.
30623807	4	69	theme	shear	822:826	arg1	value					840:844	a hydraulic shear strength GT value	810:844	a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280	810:902	The results showed that activated sludge flocs broken at a hydraulic shear strength GT value (the product of the velocity gradient and time) of 56,280 exhibited enhanced re-growth performance compared with the control.
30623807	1	70	theme	hydraulic	134:142	arg1	action					153:158	hydraulic shearing action	134:158	hydraulic shearing action	134:158	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30623807	6	71	from	sludge	1213:1218	arg1	similar					1233:1239	similar	1233:1239	similar	1233:1239	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	6	71	from	sludge	1213:1218	arg1	spectrum					1180:1187	the spectrum	1176:1187	the spectrum of loosely bound EPS in sludge	1176:1218	FTIR revealed that, after breakage, the spectrum of loosely bound EPS in sludge tended to be similar to that of tightly bound EPS.
30623807	1	72	theme	re-growth	229:237	arg1	performances					239:250	floc aggregation, breakage and re-growth performances	198:250	floc aggregation, breakage and re-growth performances under different shear strengths	198:282	In order to evaluate the effects of hydraulic shearing action on activated sludge floc aggregation, floc aggregation, breakage and re-growth performances under different shear strengths were systematically examined.
30326226	4	0	theme	antioxidant	478:488	arg1	activity					490:497	the antioxidant activity	474:497	the antioxidant activity of WSP1	474:505	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	5	1	from	fucose	754:759	arg1	ratio					786:790	a molar ratio	778:790	a molar ratio of 1:3.09	778:800	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	0	2	theme	polysaccharide	82:95	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	0	2	theme	polysaccharide	82:95	arg1	characterization					21:36	molecular characterization	11:36	molecular characterization	11:36	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	0	2	theme	polysaccharide	82:95	arg1	activity					54:61	antioxidant activity	42:61	antioxidant activity	42:61	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	0	2	theme	polysaccharide	82:95	arg1	Berk					160:163	Berk	160:163	Berk	160:163	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	4	3	theme	WSP1	502:505	arg1	activity					490:497	the antioxidant activity	474:497	the antioxidant activity of WSP1	474:505	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	5	4	theme	molecular	688:696	arg1	9 kDa					717:721	9 kDa	717:721	9 kDa	717:721	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	5	4	theme	molecular	688:696	arg1	weight					698:703	the molecular weight	684:703	the molecular weight of WSP1	684:711	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	6	5	theme	methylation	840:850	arg1	analysis					852:859	methylation analysis	840:859	methylation analysis	840:859	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	4	6	theme	scavenging	562:571	arg1	ability					573:579	scavenging ability	562:579	scavenging ability on DPPH radical and hydroxyl radical	562:616	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	3	7	theme	UV	380:381	arg1	spectroscopy					383:394	UV spectroscopy	380:394	UV spectroscopy	380:394	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	3	7	theme	UV	380:381	arg1	GPC					368:370	GPC	368:370	GPC	368:370	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	6	8	theme	→2-α-l-Fucp-1→	932:945	arg1	3→					961:962	→2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	932:962	follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	923:962	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	4	9	from	power	555:559	arg1	radical					610:616	hydroxyl radical	601:616	hydroxyl radical	601:616	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	9	from	power	555:559	arg1	ion					652:654	ferrous ion	644:654	ferrous ion	644:654	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	9	from	power	555:559	arg1	radical					589:595	DPPH radical	584:595	DPPH radical	584:595	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	5	10	from	galactose	765:773	arg1	ratio					786:790	a molar ratio	778:790	a molar ratio of 1:3.09	778:800	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	7	11	theme	concentration	1009:1021	arg1	range					1023:1027	the concentration range	1005:1027	the concentration range tested in this experiment	1005:1053	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	12	contain	had	1061:1063	arg2	ability					1083:1089	strong scavenging ability	1065:1089	strong scavenging ability	1065:1089	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	12	contain	had	1061:1063	arg1	WSP1					1056:1059	WSP1	1056:1059	WSP1	1056:1059	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	4	13	from	ability	633:639	arg1	radical					610:616	hydroxyl radical	601:616	hydroxyl radical	601:616	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	13	from	ability	633:639	arg1	ion					652:654	ferrous ion	644:654	ferrous ion	644:654	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	13	from	ability	633:639	arg1	radical					589:595	DPPH radical	584:595	DPPH radical	584:595	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	14	theme	chelating	623:631	arg1	ability					633:639	chelating ability	623:639	chelating ability on ferrous ion	623:654	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	0	15	theme	fruiting	116:123	arg1	body					125:128	the fruiting body	112:128	the fruiting body of Termitornyces albuminosus	112:157	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	6	16	theme	monosaccharide	812:825	arg1	composition					827:837	monosaccharide composition	812:837	monosaccharide composition	812:837	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	5	17	theme	1:3.09	795:800	arg1	ratio					786:790	a molar ratio	778:790	a molar ratio of 1:3.09	778:800	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	3	18	theme	FT-IR	397:401	arg1	spectroscopy					403:414	FT-IR spectroscopy	397:414	FT-IR spectroscopy	397:414	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	3	18	theme	FT-IR	397:401	arg1	GPC					368:370	GPC	368:370	GPC	368:370	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	4	19	from	ability	573:579	arg1	radical					610:616	hydroxyl radical	601:616	hydroxyl radical	601:616	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	19	from	ability	573:579	arg1	ion					652:654	ferrous ion	644:654	ferrous ion	644:654	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	19	from	ability	573:579	arg1	radical					589:595	DPPH radical	584:595	DPPH radical	584:595	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	7	20	theme	DPPH	1094:1097	arg1	radical					1099:1105	DPPH radical	1094:1105	DPPH radical	1094:1105	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	21	theme	scavenging	1072:1081	arg1	ability					1083:1089	strong scavenging ability	1065:1089	strong scavenging ability	1065:1089	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	6	22	theme	WSP1	901:904	arg1	follows					923:929	follows	923:929	follows	923:929	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	6	22	theme	WSP1	901:904	arg1	unit					893:896	the possible repeating unit	870:896	the possible repeating unit of WSP1	870:904	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	4	23	theme	hydroxyl	601:608	arg1	radical					610:616	hydroxyl radical	601:616	hydroxyl radical	601:616	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	0	24	theme	molecular	11:19	arg1	characterization					21:36	molecular characterization	11:36	molecular characterization	11:36	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	3	25	theme	monosaccharide	298:311	arg1	composition					313:323	monosaccharide composition	298:323	monosaccharide composition	298:323	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	2	26	theme	polysaccharide	189:202	arg1	WSP1					204:207	A water-soluble polysaccharide WSP1	173:207	A water-soluble polysaccharide WSP1	173:207	A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus.
30326226	0	27	theme	antioxidant	42:52	arg1	activity					54:61	antioxidant activity	42:61	antioxidant activity	42:61	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	3	28	theme	molecular	280:288	arg1	weight					290:295	Its molecular weight	276:295	Its molecular weight	276:295	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	2	29	theme	water-soluble	175:187	arg1	WSP1					204:207	A water-soluble polysaccharide WSP1	173:207	A water-soluble polysaccharide WSP1	173:207	A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus.
30326226	3	30	dep	NMR	439:441	arg1	1D					444:445	1D	444:445	1D	444:445	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	3	30	dep	NMR	439:441	arg1	2D					451:452	2D	451:452	2D	451:452	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	5	31	theme	molar	780:784	arg1	ratio					786:790	a molar ratio	778:790	a molar ratio of 1:3.09	778:800	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	7	32	theme	strong	1065:1070	arg1	ability					1083:1089	strong scavenging ability	1065:1089	strong scavenging ability	1065:1089	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	6	33	theme	repeating	883:891	arg1	follows					923:929	follows	923:929	follows	923:929	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	6	33	theme	repeating	883:891	arg1	unit					893:896	the possible repeating unit	870:896	the possible repeating unit of WSP1	870:904	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	0	34	theme	albuminosus	147:157	arg1	body					125:128	the fruiting body	112:128	the fruiting body of Termitornyces albuminosus	112:157	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	5	35	theme	WSP1	708:711	arg1	9 kDa					717:721	9 kDa	717:721	9 kDa	717:721	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	5	35	theme	WSP1	708:711	arg1	weight					698:703	the molecular weight	684:703	the molecular weight of WSP1	684:711	The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09.
30326226	6	36	theme	possible	874:881	arg1	follows					923:929	follows	923:929	follows	923:929	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	6	36	theme	possible	874:881	arg1	unit					893:896	the possible repeating unit	870:896	the possible repeating unit of WSP1	870:904	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	3	37	theme	molecular	329:337	arg1	structure					339:347	molecular structure	329:347	molecular structure	329:347	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	2	38	theme	fruiting	232:239	arg1	body					241:244	the fruiting body	228:244	the fruiting body of Termitornyces albuminosus	228:273	A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus.
30326226	3	39	theme	methylation	417:427	arg1	GPC					368:370	GPC	368:370	GPC	368:370	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	3	39	theme	methylation	417:427	arg1	analysis					429:436	methylation analysis	417:436	methylation analysis	417:436	Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC-MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM.
30326226	6	40	dep	follows	923:929	arg1	3→					961:962	→2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	932:962	follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	923:962	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	7	41	theme	antioxidant	969:979	arg1	assay					981:985	The antioxidant assay	965:985	The antioxidant assay	965:985	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	42	theme	radical	1169:1175	arg1	scavenger					1177:1185	a powerful radical scavenger	1158:1185	a powerful radical scavenger	1158:1185	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	42	theme	radical	1169:1175	arg1	WSP1					1124:1127	WSP1	1124:1127	WSP1	1124:1127	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	43	used	used	1150:1153	arg2	scavenger					1177:1185	a powerful radical scavenger	1158:1185	a powerful radical scavenger	1158:1185	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	43	used	used	1150:1153	arg2	WSP1					1124:1127	WSP1	1124:1127	WSP1	1124:1127	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	44	theme	powerful	1160:1167	arg1	scavenger					1177:1185	a powerful radical scavenger	1158:1185	a powerful radical scavenger	1158:1185	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	7	44	theme	powerful	1160:1167	arg1	WSP1					1124:1127	WSP1	1124:1127	WSP1	1124:1127	The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
30326226	6	45	theme	6-α-d-Galp-1	948:959	arg1	3→					961:962	→2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	932:962	follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→	923:962	Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows: →2-α-l-Fucp-1→ (6-α-d-Galp-1)3→.
30326226	0	46	theme	water-soluble	68:80	arg1	polysaccharide					82:95	a water-soluble polysaccharide	66:95	a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus	66:157	Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.)
30326226	2	47	theme	albuminosus	263:273	arg1	body					241:244	the fruiting body	228:244	the fruiting body of Termitornyces albuminosus	228:273	A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus.
30326226	2	48	theme	Termitornyces	249:261	arg1	albuminosus					263:273	Termitornyces albuminosus	249:273	Termitornyces albuminosus	249:273	A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus.
30326226	4	49	theme	ferrous	644:650	arg1	ion					652:654	ferrous ion	644:654	ferrous ion	644:654	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
30326226	4	50	theme	DPPH	584:587	arg1	radical					589:595	DPPH radical	584:595	DPPH radical	584:595	Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion.
31566961	0	0	theme	Methyl	108:113	arg1	Derivatives					123:133	Methyl Alditol Derivatives	108:133	Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry	108:174	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	2	1	dep	composition	547:557	arg1	methods					559:565	methods	559:565	the common glycosyl composition methods	527:565	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	1	2	theme	monosaccharide	194:207	arg1	composition					209:219	the monosaccharide composition	190:219	the monosaccharide composition of plant and microbial cell wall polysaccharides	190:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	8	3	theme	polysaccharides	1626:1640	arg1	analysis					1604:1611	composition analysis	1592:1611	composition analysis of insoluble polysaccharides	1592:1640	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	4	4	theme	stable	986:991	arg1	residues					993:1000	less stable residues	981:1000	less stable residues	981:1000	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	5	5	theme	initial	1198:1204	arg1	step					1221:1224	the initial derivatization step	1194:1224	the initial derivatization step	1194:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	4	6	theme	harsh	942:946	arg1	conditions					948:957	very harsh conditions	937:957	very harsh conditions	937:957	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	6	7	theme	depolymerization	1426:1441	arg1	efficiency					1393:1402	the efficiency	1389:1402	the efficiency of the acid-catalyzed depolymerization	1389:1441	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	1	8	theme	composition	209:219	arg1	Knowledge					177:185	Knowledge	177:185	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides	177:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	1	9	theme	polysaccharide	307:320	arg1	structure					322:330	polysaccharide structure	307:330	polysaccharide structure	307:330	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	5	10	theme	derivatization	1206:1219	arg1	step					1221:1224	the initial derivatization step	1194:1224	the initial derivatization step	1194:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	5	11	theme	new	1021:1023	arg1	approach					1025:1032	a new approach	1019:1032	a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step	1019:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	1	12	theme	structure	322:330	arg1	understanding					290:302	the understanding	286:302	the understanding of polysaccharide structure and function	286:343	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	0	13	theme	Alditol	115:121	arg1	Derivatives					123:133	Methyl Alditol Derivatives	108:133	Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry	108:174	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	7	14	theme	GC/MS	1552:1556	arg1	analysis					1558:1565	GC/MS analysis	1552:1565	GC/MS analysis	1552:1565	After the hydrolysis, the partially methylated monosaccharides are reduced to alditols and remethylated for GC/MS analysis.
31566961	3	15	from	insoluble	606:614	arg1	addition					571:578	addition	571:578	addition	571:578	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	8	16	theme	types	1736:1740	arg1	quantification					1714:1727	quantification	1714:1727	quantification of all types of sugars, including uronic acids,	1714:1775	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	0	17	theme	Gas	139:141	arg1	Spectrometry					163:174	Gas Chromatography/Mass Spectrometry	139:174	Gas Chromatography/Mass Spectrometry	139:174	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	8	18	theme	insoluble	1616:1624	arg1	polysaccharides					1626:1640	insoluble polysaccharides	1616:1640	insoluble polysaccharides	1616:1640	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	8	19	theme	same	1784:1787	arg1	column					1789:1794	the same column	1780:1794	the same column	1780:1794	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	2	20	theme	glycosyl	538:545	arg1	composition					547:557	the common glycosyl composition methods	527:565	the common glycosyl composition methods	527:565	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	1	21	theme	plant	224:228	arg1	polysaccharides					254:268	plant and microbial cell wall polysaccharides	224:268	polysaccharides	254:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	0	22	theme	Monosaccharide	14:27	arg1	Analysis					41:48	Comprehensive Monosaccharide Composition Analysis	0:48	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.	0:175	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	6	23	theme	key	1231:1233	arg1	finding					1235:1241	Our key finding	1227:1241	Our key finding	1227:1241	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	3	24	from	addition	571:578	arg1	insoluble					606:614	insoluble	606:614	insoluble	606:614	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	3	25	theme	viscous	651:657	arg1	gels					659:662	highly viscous gels	644:662	highly viscous gels	644:662	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	8	26	dep	enabling	1583:1590	arg1	addition					1571:1578	addition	1571:1578	addition	1571:1578	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	0	27	theme	Comprehensive	0:12	arg1	Analysis					41:48	Comprehensive Monosaccharide Composition Analysis	0:48	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.	0:175	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	1	28	theme	function	336:343	arg1	understanding					290:302	the understanding	286:302	the understanding of polysaccharide structure and function	286:343	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	4	29	theme	composition	807:817	arg1	analysis					819:826	monosaccharide composition analysis	792:826	monosaccharide composition analysis	792:826	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	8	30	theme	response	1817:1824	arg1	factors					1826:1832	consistent response factors	1806:1832	consistent response factors for different monosaccharide classes	1806:1869	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	1	31	theme	microbial	234:242	arg1	polysaccharides					254:268	plant and microbial cell wall polysaccharides	224:268	polysaccharides	254:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	4	32	theme	residues	993:1000	arg1	loss					973:976	loss	973:976	loss of less stable residues	973:1000	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	2	33	theme	hydrolytic	365:374	arg1	stability					376:384	the hydrolytic stability	361:384	the hydrolytic stability of the glycosidic bonds	361:408	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	5	34	theme	polysaccharides	1091:1105	arg1	analysis					1079:1086	the monosaccharide composition analysis	1048:1086	the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step	1048:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	2	35	theme	signal	508:513	arg1	response					515:522	signal response	508:522	signal response	508:522	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	2	36	theme	response	515:522	arg1	inconsistency					491:503	inconsistency	491:503	inconsistency of signal response in the common glycosyl composition methods	491:565	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	0	37	theme	Chromatography/Mass	143:161	arg1	Spectrometry					163:174	Gas Chromatography/Mass Spectrometry	139:174	Gas Chromatography/Mass Spectrometry	139:174	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	0	38	dep	Permethylation	82:95	arg1	Produce					100:106	Produce	100:106	To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry	97:174	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	1	39	theme	wall	249:252	arg1	polysaccharides					254:268	plant and microbial cell wall polysaccharides	224:268	polysaccharides	254:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	0	40	theme	Insoluble	53:61	arg1	Polysaccharides					63:77	Insoluble Polysaccharides	53:77	Insoluble Polysaccharides	53:77	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	3	41	theme	many	581:584	arg1	polysaccharides					586:600	many polysaccharides	581:600	many polysaccharides	581:600	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	8	42	theme	sugars	1745:1750	arg1	types					1736:1740	all types	1732:1740	all types	1732:1740	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	8	42	theme	sugars	1745:1750	arg1	acids					1770:1774	uronic acids	1763:1774	uronic acids	1763:1774	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	4	43	theme	samples	896:902	arg1	portions					873:880	the soluble portions	861:880	the soluble portions of biological samples	861:902	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	2	44	theme	monosaccharides	439:453	arg1	susceptibility					421:434	the susceptibility	417:434	the susceptibility of monosaccharides to acid-catalyzed degradation	417:483	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	1	45	theme	polysaccharides	254:268	arg1	composition					209:219	the monosaccharide composition	190:219	the monosaccharide composition of plant and microbial cell wall polysaccharides	190:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	4	46	theme	biological	885:894	arg1	samples					896:902	biological samples	885:902	biological samples	885:902	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	5	47	from	permethylation	1168:1181	arg1	DMSO					1186:1189	DMSO	1186:1189	DMSO	1186:1189	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	6	48	theme	acid-catalyzed	1411:1424	arg1	depolymerization					1426:1441	the acid-catalyzed depolymerization	1407:1441	the acid-catalyzed depolymerization	1407:1441	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	8	49	contain	has	1660:1662	arg1	method					1648:1653	this method	1643:1653	this method	1643:1653	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	8	49	contain	has	1660:1662	arg2	advantages					1668:1677	the advantages	1664:1677	the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column	1664:1794	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	8	50	theme	different	1838:1846	arg1	classes					1863:1869	different monosaccharide classes	1838:1869	different monosaccharide classes	1838:1869	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	0	51	theme	Polysaccharides	63:77	arg1	Analysis					41:48	Comprehensive Monosaccharide Composition Analysis	0:48	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.	0:175	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	2	52	theme	bonds	404:408	arg1	stability					376:384	the hydrolytic stability	361:384	the hydrolytic stability of the glycosidic bonds	361:408	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	6	53	from	factor	1379:1384	arg1	efficiency					1393:1402	the efficiency	1389:1402	the efficiency of the acid-catalyzed depolymerization	1389:1441	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	8	54	theme	monosaccharide	1848:1861	arg1	classes					1863:1869	different monosaccharide classes	1838:1869	different monosaccharide classes	1838:1869	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	5	55	theme	monosaccharide	1052:1065	arg1	analysis					1079:1086	the monosaccharide composition analysis	1048:1086	the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step	1048:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	4	56	theme	soluble	865:871	arg1	portions					873:880	the soluble portions	861:880	the soluble portions of biological samples	861:902	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	6	57	theme	hydrolysis	1312:1321	arg1	step					1323:1326	the hydrolysis step	1308:1326	the hydrolysis step	1308:1326	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	2	58	theme	glycosidic	393:402	arg1	bonds					404:408	the glycosidic bonds	389:408	the glycosidic bonds	389:408	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	3	59	dep	insoluble	606:614	arg1	soluble					627:633	soluble	627:633	soluble	627:633	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	7	60	theme	methylated	1480:1489	arg1	monosaccharides					1491:1505	the partially methylated monosaccharides	1466:1505	the partially methylated monosaccharides	1466:1505	After the hydrolysis, the partially methylated monosaccharides are reduced to alditols and remethylated for GC/MS analysis.
31566961	3	61	theme	traditional	726:736	arg1	methods					738:744	traditional methods	726:744	traditional methods	726:744	In addition, many polysaccharides are insoluble, partially soluble, or form highly viscous gels in water, and this also hinders or even prevents detection by traditional methods.
31566961	5	62	theme	composition	1067:1077	arg1	analysis					1079:1086	the monosaccharide composition analysis	1048:1086	the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step	1048:1224	Here we present a new approach to accomplish the monosaccharide composition analysis of polysaccharides, including those that are not or sparingly soluble, based on permethylation in DMSO as the initial derivatization step.
31566961	2	63	from	inconsistency	491:503	arg1	composition					547:557	the common glycosyl composition methods	527:565	the common glycosyl composition methods	527:565	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	8	64	theme	consistent	1806:1815	arg1	factors					1826:1832	consistent response factors	1806:1832	consistent response factors for different monosaccharide classes	1806:1869	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	8	65	theme	uronic	1763:1768	arg1	acids					1770:1774	uronic acids	1763:1774	uronic acids	1763:1774	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	4	66	theme	monosaccharide	792:805	arg1	analysis					819:826	monosaccharide composition analysis	792:826	monosaccharide composition analysis	792:826	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
31566961	2	67	from	Differences	346:356	arg1	susceptibility					421:434	the susceptibility	417:434	the susceptibility of monosaccharides to acid-catalyzed degradation	417:483	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	2	67	from	Differences	346:356	arg1	stability					376:384	the hydrolytic stability	361:384	the hydrolytic stability of the glycosidic bonds	361:408	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	1	68	theme	cell	244:247	arg1	polysaccharides					254:268	plant and microbial cell wall polysaccharides	224:268	polysaccharides	254:268	Knowledge of the monosaccharide composition of plant and microbial cell wall polysaccharides is critical for the understanding of polysaccharide structure and function.
31566961	2	69	theme	acid-catalyzed	458:471	arg1	degradation					473:483	acid-catalyzed degradation	458:483	acid-catalyzed degradation	458:483	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	6	70	from	differences	1337:1347	arg1	solubility					1352:1361	solubility	1352:1361	solubility	1352:1361	Our key finding is that the permethylation solubilizes the polysaccharide before the hydrolysis step, so that differences in solubility are no longer a factor in the efficiency of the acid-catalyzed depolymerization.
31566961	8	71	theme	composition	1592:1602	arg1	analysis					1604:1611	composition analysis	1592:1611	composition analysis of insoluble polysaccharides	1592:1640	In addition to enabling composition analysis of insoluble polysaccharides, this method also has the advantages that it is comprehensive, allowing quantification of all types of sugars, including uronic acids, on the same column and gives consistent response factors for different monosaccharide classes.
31566961	0	72	theme	Composition	29:39	arg1	Analysis					41:48	Comprehensive Monosaccharide Composition Analysis	0:48	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.	0:175	Comprehensive Monosaccharide Composition Analysis of Insoluble Polysaccharides by Permethylation To Produce Methyl Alditol Derivatives for Gas Chromatography/Mass Spectrometry.
31566961	2	73	theme	common	531:536	arg1	composition					547:557	the common glycosyl composition methods	527:565	the common glycosyl composition methods	527:565	Differences in the hydrolytic stability of the glycosidic bonds and in the susceptibility of monosaccharides to acid-catalyzed degradation cause inconsistency of signal response in the common glycosyl composition methods.
31566961	4	74	theme	available	770:778	arg1	methods					780:786	currently available methods	760:786	currently available methods for monosaccharide composition analysis	760:826	As a result, currently available methods for monosaccharide composition analysis lack accuracy and are limited to the soluble portions of biological samples or expose the polysaccharides to very harsh conditions, resulting in loss of less stable residues.
30243245	0	0	theme	based	90:94	arg1	frameworks					110:119	highly stable zirconium based metal-organic frameworks	66:119	highly stable zirconium based metal-organic frameworks	66:119	Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks.
30243245	6	1	theme	resulted	900:907	arg1	heterogeneity					909:921	the resulted heterogeneity	896:921	the resulted heterogeneity	896:921	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	1	2	theme	enhanced	175:182	arg1	stability					184:192	enhanced stability	175:192	enhanced stability	175:192	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	0	3	theme	zirconium	80:88	arg1	frameworks					110:119	highly stable zirconium based metal-organic frameworks	66:119	highly stable zirconium based metal-organic frameworks	66:119	Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks.
30243245	5	4	theme	85	699:700	arg1	%					701:701	%	701:701	%	701:701	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	3	5	theme	NH2	548:550	arg1	groups					552:557	NH2 groups	548:557	NH2 groups	548:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	1	6	with	system	163:168	arg1	reusability					198:208	reusability	198:208	reusability	198:208	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	1	6	with	system	163:168	arg1	stability					184:192	enhanced stability	175:192	enhanced stability	175:192	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	5	7	theme	maximum	650:656	arg1	activity					658:665	The maximum activity	646:665	The maximum activity attained at 55 °C	646:683	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	5	7	theme	maximum	650:656	arg1	kept					694:697	kept	694:697	kept 85%	694:701	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	0	8	theme	metal-organic	96:108	arg1	frameworks					110:119	highly stable zirconium based metal-organic frameworks	66:119	highly stable zirconium based metal-organic frameworks	66:119	Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks.
30243245	2	9	theme	carboxymethyl	392:404	arg1	cellulose					406:414	carboxymethyl cellulose	392:414	carboxymethyl cellulose	392:414	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
30243245	3	10	theme	extra	526:530	arg1	sites					539:543	the extra anchor sites	522:543	the extra anchor sites of NH2 groups	522:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	3	10	theme	extra	526:530	arg1	groups					552:557	NH2 groups	548:557	NH2 groups	548:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	7	11	theme	practical	1118:1126	arg1	application					1128:1138	practical application	1118:1138	practical application	1118:1138	The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
30243245	2	12	theme	cellulase	305:313	arg1	composites					315:324	two cellulase composites	301:324	two cellulase composites	301:324	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
30243245	3	13	theme	precursor	497:505	arg1	UiO-66					507:512	the precursor UiO-66	493:512	the precursor UiO-66	493:512	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	3	14	theme	functionalized	425:438	arg1	MOF					451:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	2	15	theme	composite	347:355	arg1	activity					335:342	the activity	331:342	the activity of composite	331:355	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
30243245	3	16	theme	UiO-66-NH2	440:449	arg1	MOF					451:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	6	17	theme	mild	975:978	arg1	centrifuge					980:989	mild centrifuge	975:989	mild centrifuge	975:989	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	1	18	theme	cellulose	214:222	arg1	hydrolysis					224:233	cellulose hydrolysis	214:233	cellulose hydrolysis	214:233	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	4	19	theme	enhanced	593:600	arg1	thermostability					602:616	enhanced thermostability	593:616	enhanced thermostability	593:616	The immobilized cellulase showed enhanced thermostability, pH tolerance and lifetime.
30243245	0	20	theme	cellulose	14:22	arg1	Hydrolysis					0:9	Hydrolysis	0:9	Hydrolysis of cellulose using cellulase	0:38	Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks.
30243245	4	21	theme	pH	619:620	arg1	tolerance					622:630	pH tolerance	619:630	pH tolerance	619:630	The immobilized cellulase showed enhanced thermostability, pH tolerance and lifetime.
30243245	1	22	theme	Developing	122:131	arg1	system					163:168	Developing a new cellulase-MOF composite system	122:168	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis	122:233	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	3	23	theme	higher	465:470	arg1	loading					480:486	higher protein loading	465:486	higher protein loading	465:486	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	5	24	theme	residual	731:738	arg1	%					758:758	72%	756:758	72% after ten cycles	756:775	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	5	24	theme	residual	731:738	arg1	activities					740:749	the residual activities	727:749	the residual activities	727:749	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	3	25	theme	protein	472:478	arg1	loading					480:486	higher protein loading	465:486	higher protein loading	465:486	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	4	26	theme	immobilized	564:574	arg1	cellulase					576:584	The immobilized cellulase	560:584	The immobilized cellulase	560:584	The immobilized cellulase showed enhanced thermostability, pH tolerance and lifetime.
30243245	7	27	theme	ultrahigh	1077:1085	arg1	activities					1087:1096	ultrahigh activities	1077:1096	ultrahigh activities	1077:1096	The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
30243245	5	28	theme	30 days	791:797	arg1	storage					799:805	30 days storage	791:805	30 days storage	791:805	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	7	29	theme	cellulase-MOF	1048:1060	arg1	composite					1062:1070	developing cellulase-MOF composite	1037:1070	developing cellulase-MOF composite	1037:1070	The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
30243245	6	30	theme	MOF	844:846	arg1	groups					834:839	The abundant NH2 and COOH groups	808:839	groups	834:839	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	6	30	theme	MOF	844:846	arg1	MOF					844:846	MOF	844:846	MOF	844:846	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	6	30	theme	MOF	844:846	arg1	NH2					821:823	The abundant NH2 and COOH groups	808:839	NH2	821:823	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	2	31	theme	adsorption	255:264	arg1	strategy					266:273	Physical adsorption strategy	246:273	Physical adsorption strategy	246:273	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
30243245	3	32	theme	anchor	532:537	arg1	sites					539:543	the extra anchor sites	522:543	the extra anchor sites of NH2 groups	522:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	3	32	theme	anchor	532:537	arg1	groups					552:557	NH2 groups	548:557	NH2 groups	548:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	7	33	theme	composite	1062:1070	arg1	future					1027:1032	future	1027:1032	future	1027:1032	The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
30243245	5	34	theme	%	701:701	arg1	activity					658:665	The maximum activity	646:665	The maximum activity attained at 55 °C	646:683	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	5	34	theme	%	701:701	arg1	kept					694:697	kept	694:697	kept 85%	694:701	The maximum activity attained at 55 °C could be kept 85% when used at 80 °C, and the residual activities were 72% after ten cycles and 65% after 30 days storage.
30243245	2	35	theme	Physical	246:253	arg1	strategy					266:273	Physical adsorption strategy	246:273	Physical adsorption strategy	246:273	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
30243245	1	36	theme	new	135:137	arg1	system					163:168	Developing a new cellulase-MOF composite system	122:168	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis	122:233	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	6	37	theme	abundant	812:819	arg1	NH2					821:823	The abundant NH2 and COOH groups	808:839	NH2	821:823	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	1	38	theme	cellulase-MOF	139:151	arg1	system					163:168	Developing a new cellulase-MOF composite system	122:168	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis	122:233	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	7	39	theme	developing	1037:1046	arg1	composite					1062:1070	developing cellulase-MOF composite	1037:1070	developing cellulase-MOF composite	1037:1070	The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.
30243245	6	40	theme	COOH	829:832	arg1	groups					834:839	The abundant NH2 and COOH groups	808:839	groups	834:839	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	6	40	theme	COOH	829:832	arg1	MOF					844:846	MOF	844:846	MOF	844:846	The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge.
30243245	1	41	theme	composite	153:161	arg1	system					163:168	Developing a new cellulase-MOF composite system	122:168	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis	122:233	Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed.
30243245	0	42	theme	stable	73:78	arg1	frameworks					110:119	highly stable zirconium based metal-organic frameworks	66:119	highly stable zirconium based metal-organic frameworks	66:119	Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks.
30243245	3	43	theme	groups	552:557	arg1	sites					539:543	the extra anchor sites	522:543	the extra anchor sites of NH2 groups	522:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	3	43	theme	groups	552:557	arg1	groups					552:557	NH2 groups	548:557	NH2 groups	548:557	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	3	44	theme	NH2	421:423	arg1	MOF					451:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF	417:453	The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups.
30243245	2	45	theme	cellulose	406:414	arg1	hydrolysis					378:387	hydrolysis	378:387	hydrolysis of carboxymethyl cellulose	378:414	Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose.
31254574	4	0	theme	antioxidant	1044:1054	arg1	activities					1056:1065	the antioxidant activities	1040:1065	the antioxidant activities of MP	1040:1071	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	1	1	theme	infrared	358:365	arg1	spectroscopy					367:378	infrared spectroscopy	358:378	infrared spectroscopy	358:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	3	2	theme	free	757:760	arg1	scavenging					770:779	excellent free radical scavenging	747:779	excellent free radical scavenging activities	747:790	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	3	theme	radical	762:768	arg1	scavenging					770:779	excellent free radical scavenging	747:779	excellent free radical scavenging activities	747:790	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	4	dep	model	463:467	arg1	model					486:490	model	486:490	model	486:490	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	5	theme	polysaccharide	82:95	arg1	activity					47:54	the antioxidant activity	31:54	the antioxidant activity of Mesona chinensis Benth polysaccharide	31:95	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	0	5	theme	polysaccharide	82:95	arg1	effect					116:121	its protective effect	101:121	its protective effect on cellular oxidative stress	101:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	2	6	theme	chain	653:657	arg1	degradation					629:639	the degradation	625:639	the degradation of the main chain of MP	625:663	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	2	6	theme	chain	653:657	arg1	inconspicuous					669:681	inconspicuous	669:681	inconspicuous	669:681	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	1	7	theme	free	473:476	arg1	radical					478:484	free radical	473:484	free radical	473:484	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	8	theme	sulfated	167:174	arg1	modification					176:187	sulfated modification	167:187	sulfated modification	167:187	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	3	9	theme	dismutase	887:895	arg1	activity					903:910	superoxide dismutase (SOD) activity	876:910	superoxide dismutase (SOD) activity	876:910	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	0	10	theme	protective	105:114	arg1	effect					116:121	its protective effect	101:121	its protective effect on cellular oxidative stress	101:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	11	theme	scavenging	770:779	arg1	activities					781:790	excellent free radical scavenging activities	747:790	excellent free radical scavenging activities	747:790	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	2	12	theme	chemical	566:573	arg1	composition					575:585	chemical composition	566:585	chemical composition	566:585	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	1	13	theme	high	381:384	arg1	chromatography					413:426	high performance anion exchange chromatography	381:426	high performance anion exchange chromatography	381:426	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	13	theme	high	381:384	arg1	Fourier					340:346	Fourier	340:346	Fourier transform infrared spectroscopy	340:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	14	from	activities	239:248	arg1	Benth					295:299	Benth	295:299	Benth	295:299	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	15	theme	performance	386:396	arg1	chromatography					413:426	high performance anion exchange chromatography	381:426	high performance anion exchange chromatography	381:426	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	15	theme	performance	386:396	arg1	Fourier					340:346	Fourier	340:346	Fourier transform infrared spectroscopy	340:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	2	16	theme	MP	662:663	arg1	chain					653:657	the main chain	644:657	the main chain of MP	644:663	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	1	17	from	effect	157:162	arg1	properties					212:221	physicochemical properties	196:221	physicochemical properties	196:221	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	17	from	effect	157:162	arg1	activities					239:248	antioxidant activities	227:248	antioxidant activities	227:248	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	4	18	theme	MP	1125:1126	arg1	application					1110:1120	application	1110:1120	application	1110:1120	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	4	18	theme	MP	1125:1126	arg1	development					1094:1104	development	1094:1104	development	1094:1104	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	1	19	theme	modification	176:187	arg1	effect					157:162	The effect	153:162	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth	153:299	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	20	from	activity	47:54	arg1	stress					145:150	cellular oxidative stress	126:150	cellular oxidative stress	126:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	21	theme	superoxide	876:885	arg1	SOD					898:900	SOD	898:900	SOD	898:900	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	21	theme	superoxide	876:885	arg1	dismutase					887:895	superoxide dismutase	876:895	superoxide dismutase (SOD) activity	876:910	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	4	22	theme	MP	1070:1071	arg1	activities					1056:1065	the antioxidant activities	1040:1065	the antioxidant activities of MP	1040:1071	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	1	23	theme	exchange	404:411	arg1	chromatography					413:426	high performance anion exchange chromatography	381:426	high performance anion exchange chromatography	381:426	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	23	theme	exchange	404:411	arg1	Fourier					340:346	Fourier	340:346	Fourier transform infrared spectroscopy	340:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	24	from	Benth	295:299	arg1	MP					269:270	MP	269:270	MP	269:270	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	24	from	Benth	295:299	arg1	polysaccharide					253:266	polysaccharide	253:266	polysaccharide (MP) from Mesona chinensis Benth	253:299	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	24	from	Benth	295:299	arg1	properties					212:221	physicochemical properties	196:221	physicochemical properties	196:221	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	24	from	Benth	295:299	arg1	activities					239:248	antioxidant activities	227:248	antioxidant activities	227:248	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	2	25	theme	significant	544:554	arg1	change					556:561	a significant change	542:561	a significant change in chemical composition and the apparent structure of MP	542:618	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	1	26	dep	Fourier	340:346	arg1	transform					348:356	transform	348:356	transform infrared spectroscopy	348:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	27	theme	Sulfated	0:7	arg1	modification					9:20	Sulfated modification	0:20	Sulfated modification	0:20	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	2	28	theme	main	648:651	arg1	chain					653:657	the main chain	644:657	the main chain of MP	644:663	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	3	29	theme	Benth	710:714	arg1	SMP					732:734	SMP	732:734	SMP	732:734	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	29	theme	Benth	710:714	arg1	polysaccharide					716:729	Sulfated Mesona chinensis Benth polysaccharide	684:729	Sulfated Mesona chinensis Benth polysaccharide (SMP)	684:735	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	30	theme	physicochemical	196:210	arg1	properties					212:221	physicochemical properties	196:221	physicochemical properties	196:221	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	31	theme	oxidative	135:143	arg1	stress					145:150	cellular oxidative stress	126:150	cellular oxidative stress	126:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	4	32	dep	development	1094:1104	arg1	the					1090:1092	the	1090:1092	the	1090:1092	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	0	33	theme	cellular	126:133	arg1	stress					145:150	cellular oxidative stress	126:150	cellular oxidative stress	126:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	2	34	theme	sulfated	513:520	arg1	modification					522:533	sulfated modification	513:533	sulfated modification	513:533	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	4	35	theme	sulfated	984:991	arg1	method					1022:1027	an effective method	1009:1027	an effective method to improve the antioxidant activities of MP	1009:1071	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	4	35	theme	sulfated	984:991	arg1	modification					993:1004	sulfated modification	984:1004	sulfated modification	984:1004	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	3	36	theme	Sulfated	684:691	arg1	SMP					732:734	SMP	732:734	SMP	732:734	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	36	theme	Sulfated	684:691	arg1	polysaccharide					716:729	Sulfated Mesona chinensis Benth polysaccharide	684:729	Sulfated Mesona chinensis Benth polysaccharide (SMP)	684:735	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	37	theme	antioxidant	227:237	arg1	activities					239:248	antioxidant activities	227:248	antioxidant activities	227:248	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	38	theme	antioxidant	35:45	arg1	activity					47:54	the antioxidant activity	31:54	the antioxidant activity of Mesona chinensis Benth polysaccharide	31:95	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	1	39	theme	chemical	322:329	arg1	methods					331:337	chemical methods	322:337	chemical methods	322:337	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	39	theme	chemical	322:329	arg1	Fourier					340:346	Fourier	340:346	Fourier transform infrared spectroscopy	340:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	40	theme	Mesona	59:64	arg1	polysaccharide					82:95	Mesona chinensis Benth polysaccharide	59:95	Mesona chinensis Benth polysaccharide	59:95	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	41	theme	MP	830:831	arg1	effect					820:825	the protective effect	805:825	the protective effect of MP on cellular oxidative stress	805:860	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	4	42	theme	effective	1012:1020	arg1	method					1022:1027	an effective method	1009:1027	an effective method to improve the antioxidant activities of MP	1009:1071	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	4	42	theme	effective	1012:1020	arg1	modification					993:1004	sulfated modification	984:1004	sulfated modification	984:1004	This study suggested that sulfated modification is an effective method to improve the antioxidant activities of MP, so as to expand the development and application of MP.
31254574	1	43	theme	anion	398:402	arg1	chromatography					413:426	high performance anion exchange chromatography	381:426	high performance anion exchange chromatography	381:426	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	43	theme	anion	398:402	arg1	Fourier					340:346	Fourier	340:346	Fourier transform infrared spectroscopy	340:378	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	3	44	theme	protective	809:818	arg1	effect					820:825	the protective effect	805:825	the protective effect of MP on cellular oxidative stress	805:860	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	45	theme	oxidative	446:454	arg1	model					463:467	oxidative stress model	446:467	oxidative stress model	446:467	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	46	theme	stress	456:461	arg1	model					463:467	oxidative stress model	446:467	oxidative stress model	446:467	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	47	from	properties	212:221	arg1	Benth					295:299	Benth	295:299	Benth	295:299	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	2	48	theme	MP	617:618	arg1	structure					604:612	the apparent structure	591:612	the apparent structure of MP	591:618	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	2	48	theme	MP	617:618	arg1	composition					575:585	chemical composition	566:585	chemical composition	566:585	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	0	49	from	effect	116:121	arg1	stress					145:150	cellular oxidative stress	126:150	cellular oxidative stress	126:150	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	50	theme	oxidative	845:853	arg1	stress					855:860	cellular oxidative stress	836:860	cellular oxidative stress	836:860	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	51	theme	Mesona	693:698	arg1	SMP					732:734	SMP	732:734	SMP	732:734	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	51	theme	Mesona	693:698	arg1	polysaccharide					716:729	Sulfated Mesona chinensis Benth polysaccharide	684:729	Sulfated Mesona chinensis Benth polysaccharide (SMP)	684:735	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	2	52	from	change	556:561	arg1	structure					604:612	the apparent structure	591:612	the apparent structure of MP	591:618	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	2	52	from	change	556:561	arg1	composition					575:585	chemical composition	566:585	chemical composition	566:585	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	3	53	from	effect	820:825	arg1	stress					855:860	cellular oxidative stress	836:860	cellular oxidative stress	836:860	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	54	theme	polysaccharide	253:266	arg1	properties					212:221	physicochemical properties	196:221	physicochemical properties	196:221	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	1	54	theme	polysaccharide	253:266	arg1	activities					239:248	antioxidant activities	227:248	antioxidant activities	227:248	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	0	55	theme	Benth	76:80	arg1	polysaccharide					82:95	Mesona chinensis Benth polysaccharide	59:95	Mesona chinensis Benth polysaccharide	59:95	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	56	theme	chinensis	700:708	arg1	SMP					732:734	SMP	732:734	SMP	732:734	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	56	theme	chinensis	700:708	arg1	polysaccharide					716:729	Sulfated Mesona chinensis Benth polysaccharide	684:729	Sulfated Mesona chinensis Benth polysaccharide (SMP)	684:735	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	1	57	dep	properties	212:221	arg1	the					192:194	the	192:194	the	192:194	The effect of sulfated modification on the physicochemical properties and antioxidant activities of polysaccharide (MP) from Mesona chinensis Benth were investigated by chemical methods, Fourier transform infrared spectroscopy, high performance anion exchange chromatography, and establishing oxidative stress model and free radical model.
31254574	2	58	theme	apparent	595:602	arg1	structure					604:612	the apparent structure	591:612	the apparent structure of MP	591:618	Results showed that sulfated modification caused a significant change in chemical composition and the apparent structure of MP, but the degradation of the main chain of MP was inconspicuous.
31254574	0	59	theme	chinensis	66:74	arg1	polysaccharide					82:95	Mesona chinensis Benth polysaccharide	59:95	Mesona chinensis Benth polysaccharide	59:95	Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress.
31254574	3	60	theme	cellular	836:843	arg1	stress					855:860	cellular oxidative stress	836:860	cellular oxidative stress	836:860	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	61	theme	malondialdehyde	927:941	arg1	content					949:955	malondialdehyde (MDA) content	927:955	malondialdehyde (MDA) content	927:955	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
31254574	3	62	theme	excellent	747:755	arg1	scavenging					770:779	excellent free radical scavenging	747:779	excellent free radical scavenging activities	747:790	Sulfated Mesona chinensis Benth polysaccharide (SMP) exhibited excellent free radical scavenging activities and improved the protective effect of MP on cellular oxidative stress by increasing superoxide dismutase (SOD) activity and decreasing malondialdehyde (MDA) content.
29428383	7	0	contain	has	1223:1225	arg2	groups					1276:1281	both α and β glycosidic bonds and massive acetyl groups	1227:1281	both α and β glycosidic bonds and massive acetyl groups	1227:1281	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	7	0	contain	has	1223:1225	arg1	PSP					1219:1221	PSP	1219:1221	PSP	1219:1221	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	1	1	theme	edodes	163:168	arg1	by-product					183:192	a by-product	181:192	a by-product of mushroom industries causing environmental pollution	181:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	1	1	theme	edodes	163:168	arg1	substrate					170:178	spent Lentinus edodes substrate	148:178	spent Lentinus edodes substrate	148:178	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	6	2	theme	reducing	1028:1035	arg1	galactose					1128:1136	galactose	1128:1136	galactose	1128:1136	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	2	theme	reducing	1028:1035	arg1	glucose					1116:1122	glucose	1116:1122	glucose	1116:1122	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	2	theme	reducing	1028:1035	arg1	sugars					1037:1042	more reducing sugars	1023:1042	more reducing sugars	1023:1042	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	1	3	theme	substrate	170:178	arg1	potential					135:143	the potential	131:143	the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution	131:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	7	4	theme	NMR	1179:1181	arg1	spectroscopy					1183:1194	FT-IR and NMR spectroscopy	1169:1194	spectroscopy	1183:1194	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	8	5	theme	polysaccharide	1463:1476	arg1	development					1436:1446	the development	1432:1446	the development of antioxidant polysaccharide from spent Lentinus edodes substrate	1432:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	4	6	theme	SLSP-F	695:700	arg1	radicals					737:744	SLSP-F on DPPH, ABTS and superoxide anion radicals	695:744	SLSP-F on DPPH, ABTS and superoxide anion radicals	695:744	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	2	7	theme	extraction	314:323	arg1	process					325:331	The extraction process	310:331	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP)	310:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	5	8	theme	purified	937:944	arg1	PSP					954:956	PSP	954:956	PSP	954:956	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	5	8	theme	purified	937:944	arg1	SLSP-F					946:951	the purified SLSP-F	933:951	the purified SLSP-F (PSP)	933:957	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	8	9	theme	Lentinus	1489:1496	arg1	substrate					1505:1513	spent Lentinus edodes substrate	1483:1513	spent Lentinus edodes substrate	1483:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	6	10	from	acids	1055:1059	arg1	composition					1073:1083	chemical composition	1064:1083	chemical composition	1064:1083	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	10	from	acids	1055:1059	arg1	contents					1096:1103	higher contents	1089:1103	higher contents of xylose	1089:1113	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	11	theme	chemical	1064:1071	arg1	composition					1073:1083	chemical composition	1064:1083	chemical composition	1064:1083	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	7	12	from	LP-F	1308:1311	arg1	different					1293:1301	different	1293:1301	different	1293:1301	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	1	13	theme	antioxidant	282:292	arg1	polysaccharide					294:307	antioxidant polysaccharide	282:307	antioxidant polysaccharide	282:307	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	7	14	theme	FT-IR	1169:1173	arg1	spectroscopy					1183:1194	FT-IR and NMR spectroscopy	1169:1194	spectroscopy	1183:1194	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	6	15	contain	has	1019:1021	arg2	acids					1055:1059	uronic acids	1048:1059	uronic acids	1048:1059	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	15	contain	has	1019:1021	arg1	PSP					1015:1017	PSP	1015:1017	PSP	1015:1017	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	15	contain	has	1019:1021	arg2	galactose					1128:1136	galactose	1128:1136	galactose	1128:1136	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	15	contain	has	1019:1021	arg2	sugars					1037:1042	more reducing sugars	1023:1042	more reducing sugars	1023:1042	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	15	contain	has	1019:1021	arg2	glucose					1116:1122	glucose	1116:1122	glucose	1116:1122	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	4	16	theme	freeze-dried	843:854	arg1	LP-F					888:891	LP-F	888:891	LP-F	888:891	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	4	16	theme	freeze-dried	843:854	arg1	polysaccharide					872:885	freeze-dried Lentinus edodes polysaccharide	843:885	freeze-dried Lentinus edodes polysaccharide (LP-F)	843:892	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	6	17	theme	more	1023:1026	arg1	galactose					1128:1136	galactose	1128:1136	galactose	1128:1136	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	17	theme	more	1023:1026	arg1	glucose					1116:1122	glucose	1116:1122	glucose	1116:1122	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	17	theme	more	1023:1026	arg1	sugars					1037:1042	more reducing sugars	1023:1042	more reducing sugars	1023:1042	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	18	theme	monosaccharide	1141:1154	arg1	composition					1156:1166	monosaccharide composition	1141:1166	monosaccharide composition	1141:1166	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	4	19	theme	EC50	680:683	arg1	0.051mg/mL					750:759	0.051mg/mL	750:759	0.051mg/mL	750:759	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	4	19	theme	EC50	680:683	arg1	values					685:690	The EC50 values	676:690	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals	676:744	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	8	20	from	substrate	1505:1513	arg1	polysaccharide					1463:1476	antioxidant polysaccharide	1451:1476	antioxidant polysaccharide from spent Lentinus edodes substrate	1451:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	8	20	from	substrate	1505:1513	arg1	development					1436:1446	the development	1432:1446	the development of antioxidant polysaccharide from spent Lentinus edodes substrate	1432:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	4	21	dep	Lentinus	856:863	arg1	edodes					865:870	edodes	865:870	edodes	865:870	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	5	22	contain	has	959:961	arg2	weight					975:980	a molecular weight	963:980	a molecular weight of 16.77kDa	963:992	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	5	22	contain	has	959:961	arg1	PSP					954:956	PSP	954:956	PSP	954:956	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	5	22	contain	has	959:961	arg1	SLSP-F					946:951	the purified SLSP-F	933:951	the purified SLSP-F (PSP)	933:957	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	6	23	theme	uronic	1048:1053	arg1	acids					1055:1059	uronic acids	1048:1059	uronic acids	1048:1059	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	7	24	theme	linked	1337:1342	arg1	residue					1353:1359	1, 3 linked α-D-Manp residue	1332:1359	1, 3 linked α-D-Manp residue with some acetyl groups	1332:1383	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	8	25	theme	edodes	1498:1503	arg1	substrate					1505:1513	spent Lentinus edodes substrate	1483:1513	spent Lentinus edodes substrate	1483:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	2	26	theme	Lentinus	342:349	arg1	edodes					351:356	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	7	27	theme	glycosidic	1240:1249	arg1	bonds					1251:1255	α and β glycosidic bonds	1232:1255	α and β glycosidic bonds	1232:1255	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	2	28	theme	morphological	463:475	arg1	property					477:484	morphological property	463:484	morphological property	463:484	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	4	29	theme	superoxide	720:729	arg1	anion					731:735	superoxide anion	720:735	superoxide anion	720:735	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	2	30	theme	spent	336:340	arg1	edodes					351:356	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	4	31	theme	radicals	737:744	arg1	0.051mg/mL					750:759	0.051mg/mL	750:759	0.051mg/mL	750:759	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	4	31	theme	radicals	737:744	arg1	values					685:690	The EC50 values	676:690	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals	676:744	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	7	32	theme	α	1232:1232	arg1	bonds					1251:1255	α and β glycosidic bonds	1232:1255	α and β glycosidic bonds	1232:1255	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	3	33	theme	best	591:594	arg1	quality					596:602	the best quality	587:602	the best quality	587:602	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	2	34	theme	chemical	441:448	arg1	composition					450:460	chemical composition	441:460	chemical composition	441:460	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	35	dep	edodes	351:356	arg1	polysaccharide					368:381	substrate polysaccharide	358:381	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	35	dep	edodes	351:356	arg1	SLSP					384:387	SLSP	384:387	SLSP	384:387	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	8	36	theme	spent	1483:1487	arg1	substrate					1505:1513	spent Lentinus edodes substrate	1483:1513	spent Lentinus edodes substrate	1483:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	7	37	theme	acetyl	1371:1376	arg1	groups					1378:1383	some acetyl groups	1366:1383	some acetyl groups	1366:1383	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	3	38	theme	activity	666:673	arg1	terms					607:611	terms	607:611	terms of the polysaccharide yield (13.00%) and antioxidant activity	607:673	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	3	39	theme	freeze-dried	550:561	arg1	SLSP-F					569:574	SLSP-F	569:574	SLSP-F	569:574	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	3	39	theme	freeze-dried	550:561	arg1	SLSP					563:566	freeze-dried SLSP	550:566	freeze-dried SLSP (SLSP-F)	550:575	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	1	40	theme	industries	206:215	arg1	by-product					183:192	a by-product	181:192	a by-product of mushroom industries causing environmental pollution	181:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	1	40	theme	industries	206:215	arg1	substrate					170:178	spent Lentinus edodes substrate	148:178	spent Lentinus edodes substrate	148:178	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	7	41	theme	acetyl	1269:1274	arg1	groups					1276:1281	both α and β glycosidic bonds and massive acetyl groups	1227:1281	both α and β glycosidic bonds and massive acetyl groups	1227:1281	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	7	42	dep	groups	1276:1281	arg1	massive					1261:1267	massive	1261:1267	massive	1261:1267	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	7	42	dep	groups	1276:1281	arg1	both					1227:1230	both	1227:1230	both	1227:1230	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	0	43	from	Lentinus	84:91	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	0	43	from	Lentinus	84:91	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity	34:53	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	0	43	from	Lentinus	84:91	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	3	44	theme	polysaccharide	620:633	arg1	yield					635:639	the polysaccharide yield	616:639	the polysaccharide yield (13.00%)	616:648	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	3	44	theme	polysaccharide	620:633	arg1	%					647:647	13.00%	642:647	13.00%	642:647	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	2	45	theme	methods	430:436	arg1	effects					412:418	the effects	408:418	the effects of drying methods on chemical composition, morphological property and antioxidant activity	408:509	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	0	46	theme	antioxidant	34:44	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity	34:53	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	7	47	with	residue	1353:1359	arg1	groups					1378:1383	some acetyl groups	1366:1383	some acetyl groups	1366:1383	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	7	48	theme	α-D-Manp	1344:1351	arg1	residue					1353:1359	1, 3 linked α-D-Manp residue	1332:1359	1, 3 linked α-D-Manp residue with some acetyl groups	1332:1383	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	8	49	theme	reliable	1410:1417	arg1	approach					1419:1426	a reliable approach	1408:1426	a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate	1408:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	3	50	theme	yield	635:639	arg1	terms					607:611	terms	607:611	terms of the polysaccharide yield (13.00%) and antioxidant activity	607:673	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	2	51	theme	drying	423:428	arg1	methods					430:436	drying methods	423:436	drying methods	423:436	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	0	52	theme	polysaccharide	58:71	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	0	52	theme	polysaccharide	58:71	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity	34:53	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	0	52	theme	polysaccharide	58:71	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	8	53	from	development	1436:1446	arg1	substrate					1505:1513	spent Lentinus edodes substrate	1483:1513	spent Lentinus edodes substrate	1483:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	3	54	theme	antioxidant	654:664	arg1	activity					666:673	antioxidant activity	654:673	antioxidant activity	654:673	Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity.
29428383	4	55	theme	Lentinus	856:863	arg1	LP-F					888:891	LP-F	888:891	LP-F	888:891	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	4	55	theme	Lentinus	856:863	arg1	polysaccharide					872:885	freeze-dried Lentinus edodes polysaccharide	843:885	freeze-dried Lentinus edodes polysaccharide (LP-F)	843:892	The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051mg/mL, 0.379mg/mL, 0.719mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F).
29428383	6	56	theme	xylose	1108:1113	arg1	composition					1073:1083	chemical composition	1064:1083	chemical composition	1064:1083	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	56	theme	xylose	1108:1113	arg1	contents					1096:1103	higher contents	1089:1103	higher contents of xylose	1089:1113	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	7	57	link	linked	1337:1342	arg1	residue					1353:1359	1, 3 linked α-D-Manp residue	1332:1359	1, 3 linked α-D-Manp residue with some acetyl groups	1332:1383	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	2	58	from	effects	412:418	arg1	composition					450:460	chemical composition	441:460	chemical composition	441:460	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	58	from	effects	412:418	arg1	property					477:484	morphological property	463:484	morphological property	463:484	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	58	from	effects	412:418	arg1	activity					502:509	antioxidant activity	490:509	antioxidant activity	490:509	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	59	theme	substrate	358:366	arg1	polysaccharide					368:381	substrate polysaccharide	358:381	spent Lentinus edodes substrate polysaccharide (SLSP)	336:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	2	59	theme	substrate	358:366	arg1	SLSP					384:387	SLSP	384:387	SLSP	384:387	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	6	60	theme	higher	1089:1094	arg1	contents					1096:1103	higher contents	1089:1103	higher contents of xylose	1089:1113	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	5	61	theme	molecular	965:973	arg1	weight					975:980	a molecular weight	963:980	a molecular weight of 16.77kDa	963:992	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	7	62	dep	both	1227:1230	arg1	bonds					1251:1255	α and β glycosidic bonds	1232:1255	α and β glycosidic bonds	1232:1255	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	2	63	theme	edodes	351:356	arg1	process					325:331	The extraction process	310:331	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP)	310:388	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	7	64	theme	β	1238:1238	arg1	bonds					1251:1255	α and β glycosidic bonds	1232:1255	α and β glycosidic bonds	1232:1255	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	0	65	theme	spent	78:82	arg1	Lentinus					84:91	spent Lentinus	78:91	spent Lentinus	78:91	Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate.
29428383	1	66	theme	environmental	225:237	arg1	pollution					239:247	environmental pollution	225:247	environmental pollution	225:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	5	67	theme	16.77kDa	985:992	arg1	weight					975:980	a molecular weight	963:980	a molecular weight of 16.77kDa	963:992	After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77kDa.
29428383	1	68	theme	mushroom	197:204	arg1	industries					206:215	mushroom industries	197:215	mushroom industries causing environmental pollution	197:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	1	69	theme	spent	148:152	arg1	by-product					183:192	a by-product	181:192	a by-product of mushroom industries causing environmental pollution	181:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	1	69	theme	spent	148:152	arg1	substrate					170:178	spent Lentinus edodes substrate	148:178	spent Lentinus edodes substrate	148:178	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	7	70	dep	has	1223:1225	arg1	different					1293:1301	different	1293:1301	different	1293:1301	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428383	2	71	theme	antioxidant	490:500	arg1	activity					502:509	antioxidant activity	490:509	antioxidant activity	490:509	The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated.
29428383	8	72	theme	antioxidant	1451:1461	arg1	polysaccharide					1463:1476	antioxidant polysaccharide	1451:1476	antioxidant polysaccharide from spent Lentinus edodes substrate	1451:1513	The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
29428383	6	73	from	sugars	1037:1042	arg1	composition					1073:1083	chemical composition	1064:1083	chemical composition	1064:1083	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	6	73	from	sugars	1037:1042	arg1	contents					1096:1103	higher contents	1089:1103	higher contents of xylose	1089:1113	Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition.
29428383	1	74	theme	Lentinus	154:161	arg1	by-product					183:192	a by-product	181:192	a by-product of mushroom industries causing environmental pollution	181:247	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	1	74	theme	Lentinus	154:161	arg1	substrate					170:178	spent Lentinus edodes substrate	148:178	spent Lentinus edodes substrate	148:178	This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide.
29428383	7	75	theme	spectroscopy	1183:1194	arg1	analysis					1196:1203	FT-IR and NMR spectroscopy analysis	1169:1203	FT-IR and NMR spectroscopy analysis	1169:1203	FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups.
29428386	4	0	theme	polysaccharides	905:919	arg1	yield					921:925	a maximum polysaccharides yield	895:925	a maximum polysaccharides yield of 22.87%	895:935	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	0	1	from	optimization	21:32	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	1	from	optimization	21:32	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	6	2	theme	reducing	1211:1218	arg1	power					1220:1224	reducing power	1211:1224	reducing power	1211:1224	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	5	3	theme	relative	1115:1122	arg1	viscosity					1124:1132	relative viscosity	1115:1132	relative viscosity	1115:1132	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	1	4	theme	surface	335:341	arg1	methodology					343:353	response surface methodology	326:353	response surface methodology (RSM)	326:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	4	theme	surface	335:341	arg1	RSM					356:358	RSM	356:358	RSM	356:358	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	6	5	theme	stronger	1202:1209	arg1	capacities					1249:1258	stronger reducing power and radical scavenging capacities	1202:1258	stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals	1202:1289	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	7	6	theme	antioxidant	1406:1416	arg1	activities					1418:1427	the antioxidant activities	1402:1427	the antioxidant activities of polysaccharides from comfrey root	1402:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	1	7	theme	comfrey	274:280	arg1	CRPs					314:317	comfrey (Symphytum officinale L.) root (CRPs)	274:318	comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	274:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	6	8	dep	showed	1195:1200	arg1	compared					1291:1298	compared	1291:1298	showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods	1195:1338	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	4	9	theme	extraction	827:836	arg1	74min					844:848	74min	844:848	74min	844:848	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	4	9	theme	extraction	827:836	arg1	time					838:841	extraction time	827:841	extraction time (74min)	827:849	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	5	10	theme	drying	948:953	arg1	methods					955:961	Different drying methods	938:961	Different drying methods	938:961	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	7	11	theme	freeze	1352:1357	arg1	method					1383:1388	a good method	1376:1388	a good method for keeping the antioxidant activities of polysaccharides from comfrey root	1376:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	7	11	theme	freeze	1352:1357	arg1	drying					1359:1364	freeze drying	1352:1364	freeze drying	1352:1364	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	3	12	from	effects	543:549	arg1	properties					665:674	physicochemical properties	649:674	physicochemical properties	649:674	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	12	from	effects	543:549	arg1	activities					692:701	antioxidant activities	680:701	antioxidant activities	680:701	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	4	13	theme	maximum	897:903	arg1	yield					921:925	a maximum polysaccharides yield	895:925	a maximum polysaccharides yield of 22.87%	895:935	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	0	14	theme	antioxidant	98:108	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	5	15	theme	CRPs	1024:1027	arg1	properties					1010:1019	the physicochemical properties	990:1019	the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight	990:1165	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	1	16	from	CRPs	314:317	arg1	polysaccharides					253:267	polysaccharides	253:267	polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	253:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	16	from	CRPs	314:317	arg1	conditions					239:248	the extraction conditions	224:248	the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	224:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	17	theme	Symphytum	283:291	arg1	comfrey					274:280	comfrey	274:280	comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	274:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	17	theme	Symphytum	283:291	arg1	L.					304:305	Symphytum officinale L.	283:305	Symphytum officinale L.	283:305	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	0	18	theme	polysaccharides	124:138	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	18	theme	polysaccharides	124:138	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	3	19	theme	antioxidant	680:690	arg1	activities					692:701	antioxidant activities	680:701	antioxidant activities	680:701	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	4	20	theme	liquid-solid	798:809	arg1	ratio					811:815	liquid-solid ratio	798:815	liquid-solid ratio (15mL/g)	798:824	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	4	20	theme	liquid-solid	798:809	arg1	15mL/g					818:823	15mL/g	818:823	15mL/g	818:823	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	4	21	theme	optimal	731:737	arg1	conditions					739:748	The optimal conditions	727:748	The optimal conditions to extract the polysaccharides	727:779	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	2	22	theme	variables	383:391	arg1	effects					366:372	The effects	362:372	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs	362:496	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	5	23	contain	had	963:965	arg2	effects					979:985	significant effects	967:985	significant effects	967:985	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	5	23	contain	had	963:965	arg1	methods					955:961	Different drying methods	938:961	Different drying methods	938:961	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	3	24	theme	physicochemical	649:663	arg1	properties					665:674	physicochemical properties	649:674	physicochemical properties	649:674	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	5	25	theme	chemical	1041:1048	arg1	composition					1050:1060	the chemical composition	1037:1060	the chemical composition (contents of total polysaccharides and uronic acid)	1037:1112	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	4	26	dep	extract	753:759	arg1	polysaccharides					765:779	the polysaccharides	761:779	the polysaccharides	761:779	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	0	27	theme	condition	11:19	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	28	from	effects	38:44	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	28	from	effects	38:44	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	6	29	dep	HD	1322:1323	arg1	methods					1332:1338	methods	1332:1338	methods	1332:1338	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	3	30	theme	CRPs	706:709	arg1	properties					665:674	physicochemical properties	649:674	physicochemical properties	649:674	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	30	theme	CRPs	706:709	arg1	activities					692:701	antioxidant activities	680:701	antioxidant activities	680:701	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	31	theme	methods	561:567	arg1	effects					543:549	the effects	539:549	the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs	539:709	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	6	32	theme	radical	1230:1236	arg1	scavenging					1238:1247	radical scavenging	1230:1247	radical scavenging	1230:1247	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	0	33	theme	Extraction	0:9	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	1	34	theme	officinale	293:302	arg1	comfrey					274:280	comfrey	274:280	comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	274:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	34	theme	officinale	293:302	arg1	L.					304:305	Symphytum officinale L.	283:305	Symphytum officinale L.	283:305	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	6	35	theme	CRPs	1168:1171	arg1	drying					1173:1178	CRPs drying	1168:1178	CRPs drying with FD method	1168:1193	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	4	36	theme	%	935:935	arg1	yield					921:925	a maximum polysaccharides yield	895:925	a maximum polysaccharides yield of 22.87%	895:935	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	6	37	theme	FD	1185:1186	arg1	method					1188:1193	FD method	1185:1193	FD method	1185:1193	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	5	38	theme	physicochemical	994:1008	arg1	properties					1010:1019	the physicochemical properties	990:1019	the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight	990:1165	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	5	39	theme	acid	1108:1111	arg1	contents					1063:1070	contents	1063:1070	contents of total polysaccharides and uronic acid	1063:1111	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	6	40	theme	CRPs	1305:1308	arg1	drying					1310:1315	CRPs drying	1305:1315	CRPs drying	1305:1315	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	2	41	theme	extraction	423:432	arg1	time					434:437	extraction time	423:437	extraction time	423:437	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	6	42	theme	DPPH	1268:1271	arg1	radicals					1282:1289	DPPH and ABTS radicals	1268:1289	radicals	1282:1289	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	3	43	theme	drying	554:559	arg1	drying					587:592	hot air drying	579:592	hot air drying (HD)	579:597	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	43	theme	drying	554:559	arg1	drying					630:635	freeze drying	623:635	freeze drying (FD)	623:640	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	43	theme	drying	554:559	arg1	drying					607:612	vacuum drying	600:612	vacuum drying (VD)	600:617	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	43	theme	drying	554:559	arg1	methods					561:567	drying methods	554:567	drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD)	554:640	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	2	44	theme	extraction	473:482	arg1	yield					484:488	the extraction yield	469:488	the extraction yield of CRPs	469:496	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	1	45	theme	root	308:311	arg1	CRPs					314:317	comfrey (Symphytum officinale L.) root (CRPs)	274:318	comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	274:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	0	46	theme	Symphytum	154:162	arg1	L.					175:176	Symphytum officinale L.	154:176	Symphytum officinale L.	154:176	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	46	theme	Symphytum	154:162	arg1	comfrey					145:151	comfrey	145:151	comfrey (Symphytum officinale L.)	145:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	47	from	properties	83:92	arg1	L.					175:176	Symphytum officinale L.	154:176	Symphytum officinale L.	154:176	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	47	from	properties	83:92	arg1	comfrey					145:151	comfrey	145:151	comfrey (Symphytum officinale L.)	145:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	7	48	theme	polysaccharides	1432:1446	arg1	activities					1418:1427	the antioxidant activities	1402:1427	the antioxidant activities of polysaccharides from comfrey root	1402:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	2	49	theme	liquid-solid	403:414	arg1	ratio					416:420	liquid-solid ratio	403:420	liquid-solid ratio	403:420	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	7	50	from	root	1461:1464	arg1	polysaccharides					1432:1446	polysaccharides	1432:1446	polysaccharides from comfrey root	1432:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	7	50	from	root	1461:1464	arg1	activities					1418:1427	the antioxidant activities	1402:1427	the antioxidant activities of polysaccharides from comfrey root	1402:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	4	51	theme	extraction	856:865	arg1	95°C					880:883	95°C	880:883	95°C	880:883	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	4	51	theme	extraction	856:865	arg1	temperature					867:877	extraction temperature	856:877	extraction temperature (95°C)	856:884	The optimal conditions to extract the polysaccharides were as follows: liquid-solid ratio (15mL/g), extraction time (74min), and extraction temperature (95°C), allowed a maximum polysaccharides yield of 22.87%.
29428386	7	52	from	activities	1418:1427	arg1	root					1461:1464	comfrey root	1453:1464	comfrey root	1453:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	5	53	theme	Different	938:946	arg1	methods					955:961	Different drying methods	938:961	Different drying methods	938:961	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	6	54	theme	ABTS	1277:1280	arg1	radicals					1282:1289	DPPH and ABTS radicals	1268:1289	radicals	1282:1289	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	0	55	theme	methods	56:62	arg1	optimization					21:32	Extraction condition optimization	0:32	Extraction condition optimization	0:32	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	55	theme	methods	56:62	arg1	effects					38:44	effects	38:44	effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.)	38:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	3	56	dep	properties	665:674	arg1	the					645:647	the	645:647	the	645:647	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	6	57	theme	scavenging	1238:1247	arg1	capacities					1249:1258	stronger reducing power and radical scavenging capacities	1202:1258	stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals	1202:1289	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	7	58	theme	comfrey	1453:1459	arg1	root					1461:1464	comfrey root	1453:1464	comfrey root	1453:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	3	59	theme	hot	579:581	arg1	drying					587:592	hot air drying	579:592	hot air drying (HD)	579:597	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	59	theme	hot	579:581	arg1	HD					595:596	HD	595:596	HD	595:596	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	2	60	from	effects	366:372	arg1	yield					484:488	the extraction yield	469:488	the extraction yield of CRPs	469:496	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	0	61	theme	drying	49:54	arg1	methods					56:62	drying methods	49:62	drying methods	49:62	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	5	62	theme	polysaccharides	1081:1095	arg1	contents					1063:1070	contents	1063:1070	contents of total polysaccharides and uronic acid	1063:1111	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	0	63	theme	officinale	164:173	arg1	L.					175:176	Symphytum officinale L.	154:176	Symphytum officinale L.	154:176	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	63	theme	officinale	164:173	arg1	comfrey					145:151	comfrey	145:151	comfrey (Symphytum officinale L.)	145:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	5	64	dep	composition	1050:1060	arg1	contents					1063:1070	contents	1063:1070	contents of total polysaccharides and uronic acid	1063:1111	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	3	65	theme	air	583:585	arg1	drying					587:592	hot air drying	579:592	hot air drying (HD)	579:597	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	65	theme	air	583:585	arg1	HD					595:596	HD	595:596	HD	595:596	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	5	66	theme	significant	967:977	arg1	effects					979:985	significant effects	967:985	significant effects	967:985	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	7	67	theme	good	1378:1381	arg1	method					1383:1388	a good method	1376:1388	a good method for keeping the antioxidant activities of polysaccharides from comfrey root	1376:1464	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	7	67	theme	good	1378:1381	arg1	drying					1359:1364	freeze drying	1352:1364	freeze drying	1352:1364	Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharides from comfrey root.
29428386	5	68	theme	total	1075:1079	arg1	polysaccharides					1081:1095	total polysaccharides	1075:1095	total polysaccharides	1075:1095	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	1	69	theme	extraction	228:237	arg1	conditions					239:248	the extraction conditions	224:248	the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	224:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	70	from	conditions	239:248	arg1	CRPs					314:317	comfrey (Symphytum officinale L.) root (CRPs)	274:318	comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	274:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	0	71	from	activities	110:119	arg1	L.					175:176	Symphytum officinale L.	154:176	Symphytum officinale L.	154:176	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	71	from	activities	110:119	arg1	comfrey					145:151	comfrey	145:151	comfrey (Symphytum officinale L.)	145:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	3	72	theme	vacuum	600:605	arg1	VD					615:616	VD	615:616	VD	615:616	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	72	theme	vacuum	600:605	arg1	drying					607:612	vacuum drying	600:612	vacuum drying (VD)	600:617	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	6	73	dep	stronger	1202:1209	arg1	power					1220:1224	reducing power	1211:1224	reducing power	1211:1224	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	5	74	theme	molecular	1150:1158	arg1	weight					1160:1165	molecular weight	1150:1165	molecular weight	1150:1165	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	0	75	theme	physicochemical	67:81	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	3	76	theme	freeze	623:628	arg1	FD					638:639	FD	638:639	FD	638:639	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	3	76	theme	freeze	623:628	arg1	drying					630:635	freeze drying	623:635	freeze drying (FD)	623:640	Moreover, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the physicochemical properties and antioxidant activities of CRPs were evaluated.
29428386	5	77	theme	uronic	1101:1106	arg1	acid					1108:1111	uronic acid	1101:1111	uronic acid	1101:1111	Different drying methods had significant effects on the physicochemical properties of CRPs such as the chemical composition (contents of total polysaccharides and uronic acid), relative viscosity, solubility and molecular weight.
29428386	0	78	from	comfrey	145:151	arg1	properties					83:92	physicochemical properties	67:92	physicochemical properties	67:92	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	78	from	comfrey	145:151	arg1	activities					110:119	antioxidant activities	98:119	antioxidant activities	98:119	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	0	78	from	comfrey	145:151	arg1	polysaccharides					124:138	polysaccharides	124:138	polysaccharides from comfrey (Symphytum officinale L.)	124:177	Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from comfrey (Symphytum officinale L.) root.
29428386	2	79	theme	CRPs	493:496	arg1	yield					484:488	the extraction yield	469:488	the extraction yield of CRPs	469:496	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	1	80	theme	response	326:333	arg1	methodology					343:353	response surface methodology	326:353	response surface methodology (RSM)	326:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	1	80	theme	response	326:333	arg1	RSM					356:358	RSM	356:358	RSM	356:358	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
29428386	2	81	theme	extraction	443:452	arg1	temperature					454:464	extraction temperature	443:464	extraction temperature	443:464	The effects of three variables including liquid-solid ratio, extraction time and extraction temperature on the extraction yield of CRPs were taken into consideration.
29428386	6	82	with	drying	1173:1178	arg1	method					1188:1193	FD method	1185:1193	FD method	1185:1193	CRPs drying with FD method showed stronger reducing power and radical scavenging capacities against DPPH and ABTS radicals compared with CRPs drying with HD and VD methods.
29428386	1	83	theme	polysaccharides	253:267	arg1	conditions					239:248	the extraction conditions	224:248	the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM)	224:359	This study was designed to investigate the extraction conditions of polysaccharides from comfrey (Symphytum officinale L.) root (CRPs) using response surface methodology (RSM).
31304048	6	0	theme	branched	972:979	arg1	glycans					981:987	other complex branched glycans	958:987	other complex branched glycans	958:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	4	1	theme	Core	605:608	arg1	glycans					612:618	sialyl Core 2 glycans	598:618	sialyl Core 2 glycans	598:618	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	4	2	theme	chemoenzymatic	528:541	arg1	strategies					543:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	6	3	theme	diverse	883:889	arg1	glycans					907:913	access diverse Core 2 O-GalNAc glycans	876:913	access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	876:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	1	4	theme	common	162:167	arg1	modifications					196:208	common protein post-translational modifications	162:208	common protein post-translational modifications in eukaryotes	162:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	4	theme	common	162:167	arg1	glycans					128:134	O-GalNAc glycans	119:134	O-GalNAc glycans	119:134	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	4	theme	common	162:167	arg1	glycans					150:156	mucin-type glycans	139:156	mucin-type glycans	139:156	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	4	5	theme	sialyl	598:603	arg1	glycans					612:618	sialyl Core 2 glycans	598:618	sialyl Core 2 glycans	598:618	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	0	6	theme	Core	103:106	arg1	Glycans					110:116	Sialyl Core 2 Glycans	96:116	Sialyl Core 2 Glycans	96:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	6	7	theme	other	958:962	arg1	glycans					981:987	other complex branched glycans	958:987	other complex branched glycans	958:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	6	8	theme	access	876:881	arg1	glycans					907:913	access diverse Core 2 O-GalNAc glycans	876:913	access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	876:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	4	9	theme	glycans	612:618	arg1	array					589:593	a diverse array	579:593	a diverse array of sialyl Core 2 glycans	579:618	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	0	10	theme	Sialyl	96:101	arg1	Glycans					110:116	Sialyl Core 2 Glycans	96:116	Sialyl Core 2 Glycans	96:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	4	11	theme	regioselective	486:499	arg1	strategies					543:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	0	12	theme	Glycans	110:116	arg1	Synthesis					83:91	Systematic Synthesis	72:91	Systematic Synthesis of Sialyl Core 2 Glycans	72:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	1	13	theme	protein	169:175	arg1	modifications					196:208	common protein post-translational modifications	162:208	common protein post-translational modifications in eukaryotes	162:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	13	theme	protein	169:175	arg1	glycans					128:134	O-GalNAc glycans	119:134	O-GalNAc glycans	119:134	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	13	theme	protein	169:175	arg1	glycans					150:156	mucin-type glycans	139:156	mucin-type glycans	139:156	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	5	14	theme	glycosylation	795:807	arg1	sequences					809:817	altered glycosylation sequences	787:817	altered glycosylation sequences	787:817	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	4	15	theme	multienzyme	509:519	arg1	strategies					543:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	6	16	theme	regioselective	849:862	arg1	strategy					864:871	a general regioselective strategy	839:871	a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	839:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	1	17	theme	post-translational	177:194	arg1	modifications					196:208	common protein post-translational modifications	162:208	common protein post-translational modifications in eukaryotes	162:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	17	theme	post-translational	177:194	arg1	glycans					128:134	O-GalNAc glycans	119:134	O-GalNAc glycans	119:134	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	17	theme	post-translational	177:194	arg1	glycans					150:156	mucin-type glycans	139:156	mucin-type glycans	139:156	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	0	18	theme	One-Pot	15:21	arg1	Strategies					57:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies	0:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans	0:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	4	19	theme	one-pot	501:507	arg1	strategies					543:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	6	20	theme	general	841:847	arg1	strategy					864:871	a general regioselective strategy	839:871	a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	839:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	2	21	theme	types	337:341	arg1	glycoproteins					305:317	glycoproteins	305:317	glycoproteins	305:317	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	2	21	theme	types	337:341	arg1	mucins					323:328	mucins	323:328	mucins	323:328	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	3	22	theme	defined	437:443	arg1	glycans					461:467	structurally defined Core 2 O-GalNAc glycans	424:467	structurally defined Core 2 O-GalNAc glycans	424:467	To better understand their biological roles, it is important to obtain structurally defined Core 2 O-GalNAc glycans.
31304048	0	23	theme	Regioselective	0:13	arg1	Strategies					57:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies	0:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans	0:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	4	24	theme	diverse	581:587	arg1	array					589:593	a diverse array	579:593	a diverse array of sialyl Core 2 glycans	579:618	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	5	25	theme	OPME	663:666	arg1	systems					668:674	OPME systems	663:674	OPME systems containing a glycosyltransferase with restricted acceptor specificity	663:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	3	26	theme	Core	445:448	arg1	glycans					461:467	structurally defined Core 2 O-GalNAc glycans	424:467	structurally defined Core 2 O-GalNAc glycans	424:467	To better understand their biological roles, it is important to obtain structurally defined Core 2 O-GalNAc glycans.
31304048	0	27	theme	Multienzyme	23:33	arg1	Strategies					57:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies	0:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans	0:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	2	28	theme	branched	253:260	arg1	glycans					241:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	2	28	theme	branched	253:260	arg1	structures					262:271	branched structures	253:271	branched structures that are broadly distributed in glycoproteins and mucins of all types of cells	253:350	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	0	29	theme	OPME	36:39	arg1	Strategies					57:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies	0:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans	0:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	5	30	dep	achieved	645:652	arg1	using					657:661	using	657:661	using OPME systems containing a glycosyltransferase with restricted acceptor specificity	657:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	5	30	dep	achieved	645:652	arg1	differentiating					752:766	differentiating	752:766	differentiating the branches using altered glycosylation sequences	752:817	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	2	31	gly	glycoproteins	305:317	arg1	glycoproteins					305:317	glycoproteins	305:317	glycoproteins	305:317	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	6	32	theme	O-GalNAc	898:905	arg1	glycans					907:913	access diverse Core 2 O-GalNAc glycans	876:913	access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	876:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	0	33	theme	Chemoenzymatic	42:55	arg1	Strategies					57:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies	0:66	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans	0:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	5	34	theme	restricted	714:723	arg1	specificity					734:744	restricted acceptor specificity	714:744	restricted acceptor specificity	714:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	6	35	theme	complex	964:970	arg1	glycans					981:987	other complex branched glycans	958:987	other complex branched glycans	958:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	2	36	theme	O-GalNAc	232:239	arg1	glycans					241:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	2	36	theme	O-GalNAc	232:239	arg1	structures					262:271	branched structures	253:271	branched structures that are broadly distributed in glycoproteins and mucins of all types of cells	253:350	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	1	37	theme	O-GalNAc	119:126	arg1	glycans					128:134	O-GalNAc glycans	119:134	O-GalNAc glycans	119:134	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	37	theme	O-GalNAc	119:126	arg1	modifications					196:208	common protein post-translational modifications	162:208	common protein post-translational modifications in eukaryotes	162:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	37	theme	O-GalNAc	119:126	arg1	glycans					150:156	mucin-type glycans	139:156	mucin-type glycans	139:156	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	6	38	theme	glycans	981:987	arg1	synthesis					945:953	the synthesis	941:953	the synthesis of other complex branched glycans	941:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	5	39	theme	acceptor	725:732	arg1	specificity					734:744	restricted acceptor specificity	714:744	restricted acceptor specificity	714:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	2	40	theme	Core	225:228	arg1	glycans					241:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans	225:247	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	2	40	theme	Core	225:228	arg1	structures					262:271	branched structures	253:271	branched structures that are broadly distributed in glycoproteins and mucins of all types of cells	253:350	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	2	41	theme	cells	346:350	arg1	types					337:341	all types	333:341	all types of cells	333:350	Core 2 O-GalNAc glycans are branched structures that are broadly distributed in glycoproteins and mucins of all types of cells.
31304048	5	42	theme	altered	787:793	arg1	sequences					809:817	altered glycosylation sequences	787:817	altered glycosylation sequences	787:817	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	1	43	from	modifications	196:208	arg1	eukaryotes					213:222	eukaryotes	213:222	eukaryotes	213:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	0	44	theme	Systematic	72:81	arg1	Synthesis					83:91	Systematic Synthesis	72:91	Systematic Synthesis of Sialyl Core 2 Glycans	72:116	Regioselective One-Pot Multienzyme (OPME) Chemoenzymatic Strategies for Systematic Synthesis of Sialyl Core 2 Glycans.
31304048	6	45	theme	Core	891:894	arg1	glycans					907:913	access diverse Core 2 O-GalNAc glycans	876:913	access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans	876:987	This work provides a general regioselective strategy to access diverse Core 2 O-GalNAc glycans which can be extended for the synthesis of other complex branched glycans.
31304048	1	46	theme	mucin-type	139:148	arg1	modifications					196:208	common protein post-translational modifications	162:208	common protein post-translational modifications in eukaryotes	162:222	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	46	theme	mucin-type	139:148	arg1	glycans					150:156	mucin-type glycans	139:156	mucin-type glycans	139:156	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	1	46	theme	mucin-type	139:148	arg1	glycans					128:134	O-GalNAc glycans	119:134	O-GalNAc glycans	119:134	O-GalNAc glycans or mucin-type glycans are common protein post-translational modifications in eukaryotes.
31304048	3	47	theme	O-GalNAc	452:459	arg1	glycans					461:467	structurally defined Core 2 O-GalNAc glycans	424:467	structurally defined Core 2 O-GalNAc glycans	424:467	To better understand their biological roles, it is important to obtain structurally defined Core 2 O-GalNAc glycans.
31304048	5	48	contain	containing	676:685	arg2	glycosyltransferase					689:707	a glycosyltransferase	687:707	a glycosyltransferase with restricted acceptor specificity	687:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	5	48	contain	containing	676:685	arg1	systems					668:674	OPME systems	663:674	OPME systems containing a glycosyltransferase with restricted acceptor specificity	663:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	4	49	theme	OPME	522:525	arg1	strategies					543:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	regioselective one-pot multienzyme (OPME) chemoenzymatic strategies	486:552	We present here regioselective one-pot multienzyme (OPME) chemoenzymatic strategies to systematically access a diverse array of sialyl Core 2 glycans.
31304048	5	50	with	glycosyltransferase	689:707	arg1	specificity					734:744	restricted acceptor specificity	714:744	restricted acceptor specificity	714:744	Regioselectivity can be achieved by using OPME systems containing a glycosyltransferase with restricted acceptor specificity or by differentiating the branches using altered glycosylation sequences.
31304048	3	51	theme	biological	380:389	arg1	roles					391:395	their biological roles	374:395	their biological roles	374:395	To better understand their biological roles, it is important to obtain structurally defined Core 2 O-GalNAc glycans.
31810277	2	0	theme	submerged	506:514	arg1	fermentation					516:527	submerged fermentation	506:527	submerged fermentation of I. obliquus	506:542	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	1	1	theme	composition	357:367	arg1	accumulation					290:301	the simultaneous accumulation	273:301	the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition	273:367	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	5	2	theme	μg/mL	1116:1120	arg1	concentration					1097:1109	the concentration	1093:1109	the concentration of 4 μg/mL	1093:1120	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	0	3	theme	Hypoglycaemic	69:81	arg1	Activity					83:90	Hypoglycaemic Activity	69:90	Hypoglycaemic Activity	69:90	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	6	4	from	activity	1367:1374	arg1	growth					1336:1341	the mycelial growth	1323:1341	the mycelial growth	1323:1341	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	7	5	theme	agents	1463:1468	arg1	addition					1429:1436	the addition	1425:1436	the addition of different stimulatory agents	1425:1468	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	2	6	theme	stimulatory	380:390	arg1	agents					392:397	Different stimulatory agents	370:397	Different stimulatory agents (VB6, VB1, betulin and birch extract)	370:435	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	6	theme	stimulatory	380:390	arg1	betulin					410:416	betulin	410:416	betulin	410:416	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	6	theme	stimulatory	380:390	arg1	extract					428:434	birch extract	422:434	birch extract	422:434	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	6	theme	stimulatory	380:390	arg1	VB1					405:407	VB1	405:407	VB1	405:407	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	6	theme	stimulatory	380:390	arg1	VB6					400:402	VB6	400:402	VB6	400:402	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	6	7	from	effect	1313:1318	arg1	growth					1336:1341	the mycelial growth	1323:1341	the mycelial growth	1323:1341	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	7	8	theme	polysaccharides	1512:1526	arg1	composition					1497:1507	the monosaccharide composition	1478:1507	the monosaccharide composition	1478:1507	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	0	9	theme	Polysaccharides	95:109	arg1	Production					54:63	Production	54:63	Production	54:63	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	0	9	theme	Polysaccharides	95:109	arg1	Activity					83:90	Hypoglycaemic Activity	69:90	Hypoglycaemic Activity	69:90	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	2	10	dep	agents	392:397	arg1	agents					392:397	Different stimulatory agents	370:397	Different stimulatory agents (VB6, VB1, betulin and birch extract)	370:435	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	10	dep	agents	392:397	arg1	betulin					410:416	betulin	410:416	betulin	410:416	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	10	dep	agents	392:397	arg1	extract					428:434	birch extract	422:434	birch extract	422:434	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	10	dep	agents	392:397	arg1	VB1					405:407	VB1	405:407	VB1	405:407	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	10	dep	agents	392:397	arg1	VB6					400:402	VB6	400:402	VB6	400:402	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	6	11	contain	had	1281:1283	arg1	extract					1273:1279	birch extract	1267:1279	birch extract	1267:1279	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	6	11	contain	had	1281:1283	arg2	activity					1367:1374	the polysaccharides activity	1347:1374	the polysaccharides activity	1347:1374	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	6	11	contain	had	1281:1283	arg2	effect					1313:1318	a significantly stimulatory effect	1285:1318	a significantly stimulatory effect on the mycelial growth	1285:1341	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	6	12	theme	mycelial	1327:1334	arg1	growth					1336:1341	the mycelial growth	1323:1341	the mycelial growth	1323:1341	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	4	13	theme	stimulatory	771:781	arg1	agents					783:788	all the four stimulatory agents	758:788	all the four stimulatory agents	758:788	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	1	14	theme	simultaneous	277:288	arg1	accumulation					290:301	the simultaneous accumulation	273:301	the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition	273:367	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	0	15	theme	Inonotus	116:123	arg1	obliquus					125:132	Inonotus obliquus	116:132	Inonotus obliquus	116:132	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	2	16	from	effects	465:471	arg1	exo-polysaccharides					483:501	active exo-polysaccharides	476:501	active exo-polysaccharides	476:501	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	6	17	theme	EPS	1239:1241	arg1	yield					1243:1247	the EPS yield	1235:1247	the EPS yield	1235:1247	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	3	18	theme	mycelial	549:556	arg1	content					582:588	The mycelial biomass, reducing sugar content	545:588	content	582:588	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	4	19	contain	had	937:939	arg1	EPS					898:900	EPS	898:900	EPS from 4 μg/mL VB1-containing medium	898:935	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	4	19	contain	had	937:939	arg2	effect					950:955	the best effect	941:955	the best effect	941:955	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	2	20	theme	I.	532:533	arg1	obliquus					535:542	I. obliquus	532:542	I. obliquus	532:542	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	7	21	theme	polysaccharide	1558:1571	arg1	activity					1573:1580	polysaccharide activity	1558:1580	polysaccharide activity	1558:1580	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	3	22	theme	biomass	558:564	arg1	content					582:588	The mycelial biomass, reducing sugar content	545:588	content	582:588	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	2	23	theme	obliquus	535:542	arg1	fermentation					516:527	submerged fermentation	506:527	submerged fermentation of I. obliquus	506:542	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	24	theme	active	476:481	arg1	exo-polysaccharides					483:501	active exo-polysaccharides	476:501	active exo-polysaccharides	476:501	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	5	25	theme	%	1146:1146	arg1	increase					1129:1136	a increase	1127:1136	a increase of 50.79% and 114.46%, respectively	1127:1172	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	7	26	theme	different	1441:1449	arg1	agents					1463:1468	different stimulatory agents	1441:1468	different stimulatory agents	1441:1468	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	6	27	contain	had	1196:1198	arg2	effect					1214:1219	a significant effect	1200:1219	a significant effect	1200:1219	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	6	27	contain	had	1196:1198	arg1	betulin					1188:1194	betulin	1188:1194	betulin	1188:1194	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	0	28	theme	Simultaneous	0:11	arg1	Use					13:15	Simultaneous Use	0:15	Simultaneous Use of Stimulatory Agents	0:37	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	5	29	theme	%	1158:1158	arg1	increase					1129:1136	a increase	1127:1136	a increase of 50.79% and 114.46%, respectively	1127:1172	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	7	30	theme	stimulatory	1451:1461	arg1	agents					1463:1468	different stimulatory agents	1441:1468	different stimulatory agents	1441:1468	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	3	31	theme	EPS	591:593	arg1	yield					595:599	EPS yield	591:599	EPS yield	591:599	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	5	32	theme	EPS	1086:1088	arg1	production					1051:1060	maximum production	1043:1060	maximum production of mycelial biomass and EPS	1043:1088	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	0	33	theme	Stimulatory	20:30	arg1	Agents					32:37	Stimulatory Agents	20:37	Stimulatory Agents	20:37	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	4	34	theme	best	945:948	arg1	effect					950:955	the best effect	941:955	the best effect	941:955	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	5	35	theme	maximum	1043:1049	arg1	production					1051:1060	maximum production	1043:1060	maximum production of mycelial biomass and EPS	1043:1088	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	3	36	theme	sugar	576:580	arg1	content					582:588	The mycelial biomass, reducing sugar content	545:588	content	582:588	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	7	37	contain	had	1535:1537	arg2	correlation					1541:1551	a correlation	1539:1551	a correlation with polysaccharide activity	1539:1580	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	7	37	contain	had	1535:1537	arg1	composition					1497:1507	the monosaccharide composition	1478:1507	the monosaccharide composition	1478:1507	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	1	38	theme	exo-polysaccharides	306:324	arg1	accumulation					290:301	the simultaneous accumulation	273:301	the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition	273:367	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	6	39	theme	stimulatory	1301:1311	arg1	effect					1313:1318	a significantly stimulatory effect	1285:1318	a significantly stimulatory effect on the mycelial growth	1285:1341	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	0	40	from	Activity	83:90	arg1	obliquus					125:132	Inonotus obliquus	116:132	Inonotus obliquus	116:132	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	0	41	dep	Production	54:63	arg1	the					50:52	the	50:52	the	50:52	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	1	42	theme	stimulatory	214:224	arg1	agents					226:231	stimulatory agents	214:231	stimulatory agents	214:231	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	3	43	theme	α-glucosidase	605:617	arg1	rate					630:633	α-glucosidase inhibition rate	605:633	α-glucosidase inhibition rate	605:633	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	7	44	theme	monosaccharide	1482:1495	arg1	composition					1497:1507	the monosaccharide composition	1478:1507	the monosaccharide composition	1478:1507	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	2	45	theme	birch	422:426	arg1	agents					392:397	Different stimulatory agents	370:397	Different stimulatory agents (VB6, VB1, betulin and birch extract)	370:435	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	45	theme	birch	422:426	arg1	extract					428:434	birch extract	422:434	birch extract	422:434	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	0	46	theme	Agents	32:37	arg1	Use					13:15	Simultaneous Use	0:15	Simultaneous Use of Stimulatory Agents	0:37	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	6	47	theme	polysaccharides	1351:1365	arg1	activity					1367:1374	the polysaccharides activity	1347:1374	the polysaccharides activity	1347:1374	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	0	48	theme	Submerged	137:145	arg1	Fermentation					147:158	Submerged Fermentation	137:158	Submerged Fermentation	137:158	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	3	49	theme	inhibition	619:628	arg1	rate					630:633	α-glucosidase inhibition rate	605:633	α-glucosidase inhibition rate	605:633	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	4	50	theme	IC50	975:978	arg1	value					980:984	an estimated IC50 value	962:984	an estimated IC50 value 24.34 μg/mL	962:996	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	4	51	theme	estimated	965:973	arg1	value					980:984	an estimated IC50 value	962:984	an estimated IC50 value 24.34 μg/mL	962:996	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	4	52	theme	VB1-containing	915:928	arg1	medium					930:935	4 μg/mL VB1-containing medium	907:935	4 μg/mL VB1-containing medium	907:935	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	0	53	from	Production	54:63	arg1	obliquus					125:132	Inonotus obliquus	116:132	Inonotus obliquus	116:132	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	4	54	theme	EPS	868:870	arg1	α-glucosidase					851:863	α-glucosidase	851:863	α-glucosidase of EPS	851:870	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	2	55	theme	Different	370:378	arg1	agents					392:397	Different stimulatory agents	370:397	Different stimulatory agents (VB6, VB1, betulin and birch extract)	370:435	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	55	theme	Different	370:378	arg1	betulin					410:416	betulin	410:416	betulin	410:416	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	55	theme	Different	370:378	arg1	extract					428:434	birch extract	422:434	birch extract	422:434	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	55	theme	Different	370:378	arg1	VB1					405:407	VB1	405:407	VB1	405:407	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	2	55	theme	Different	370:378	arg1	VB6					400:402	VB6	400:402	VB6	400:402	Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus.
31810277	4	56	theme	μg/mL	909:913	arg1	medium					930:935	4 μg/mL VB1-containing medium	907:935	4 μg/mL VB1-containing medium	907:935	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	4	57	from	medium	930:935	arg1	EPS					898:900	EPS	898:900	EPS from 4 μg/mL VB1-containing medium	898:935	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	4	58	theme	inhibitory	823:832	arg1	activity					834:841	the inhibitory activity	819:841	the inhibitory activity against α-glucosidase of EPS than the control	819:887	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	6	59	theme	birch	1267:1271	arg1	extract					1273:1279	birch extract	1267:1279	birch extract	1267:1279	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	3	60	theme	monosaccharide	690:703	arg1	composition					705:715	monosaccharide composition	690:715	monosaccharide composition	690:715	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	6	61	theme	significant	1202:1212	arg1	effect					1214:1219	a significant effect	1200:1219	a significant effect	1200:1219	In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1.
31810277	7	62	with	correlation	1541:1551	arg1	activity					1573:1580	polysaccharide activity	1558:1580	polysaccharide activity	1558:1580	Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.
31810277	5	63	theme	mycelial	1065:1072	arg1	biomass					1074:1080	mycelial biomass	1065:1080	mycelial biomass	1065:1080	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	4	64	theme	agents	783:788	arg1	addition					746:753	the addition	742:753	the addition of all the four stimulatory agents	742:788	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	1	65	theme	liquid	236:241	arg1	obliquus					261:268	liquid cultured Inonotus obliquus	236:268	liquid cultured Inonotus obliquus	236:268	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	4	66	theme	μg/mL	992:996	arg1	value					980:984	an estimated IC50 value	962:984	an estimated IC50 value 24.34 μg/mL	962:996	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	5	67	theme	biomass	1074:1080	arg1	production					1051:1060	maximum production	1043:1060	maximum production of mycelial biomass and EPS	1043:1088	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	5	68	theme	stimulatory	1014:1024	arg1	agents					1026:1031	the four stimulatory agents	1005:1031	the four stimulatory agents	1005:1031	Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively.
31810277	1	69	from	effect	195:200	arg1	accumulation					290:301	the simultaneous accumulation	273:301	the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition	273:367	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	1	70	theme	cultured	243:250	arg1	obliquus					261:268	liquid cultured Inonotus obliquus	236:268	liquid cultured Inonotus obliquus	236:268	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	4	71	theme	24.34	986:990	arg1	μg/mL					992:996	μg/mL	992:996	μg/mL	992:996	The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL.
31810277	1	72	theme	monosaccharide	342:355	arg1	composition					357:367	their monosaccharide composition	336:367	their monosaccharide composition	336:367	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
31810277	0	73	from	obliquus	125:132	arg1	Polysaccharides					95:109	Polysaccharides	95:109	Polysaccharides from Inonotus obliquus	95:132	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	0	73	from	obliquus	125:132	arg1	Production					54:63	Production	54:63	Production	54:63	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	0	73	from	obliquus	125:132	arg1	Activity					83:90	Hypoglycaemic Activity	69:90	Hypoglycaemic Activity	69:90	Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation.
31810277	3	74	dep	content	582:588	arg1	reducing					567:574	The mycelial biomass, reducing sugar content	545:588	reducing	567:574	The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition.
31810277	1	75	theme	Inonotus	252:259	arg1	obliquus					261:268	liquid cultured Inonotus obliquus	236:268	liquid cultured Inonotus obliquus	236:268	This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition.
29342936	7	0	theme	anti-inflammatory	1324:1340	arg1	effective					1371:1379	effective	1371:1379	effective	1371:1379	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	0	theme	anti-inflammatory	1324:1340	arg1	activities					1342:1351	The anti-inflammatory activities	1320:1351	The anti-inflammatory activities of HAPS	1320:1359	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	3	1	theme	light	739:743	arg1	scattering					745:754	evaporative light scattering	727:754	evaporative light scattering detection (HPGFC-ELSD)	727:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	8	2	theme	bioactivity	1585:1595	arg1	improvement					1597:1607	the anti-inflammatory and bioactivity improvement	1559:1607	the anti-inflammatory and bioactivity improvement	1559:1607	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	6	3	theme	FT-IR	1227:1231	arg1	analysis					1241:1248	FT-IR and NMR analysis	1227:1248	analysis	1241:1248	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	2	4	theme	tonic	375:379	arg1	effect					381:386	the tonic effect	371:386	the tonic effect of Radix Astragalus	371:406	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	4	5	theme	ultra-performance	829:845	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	5	theme	ultra-performance	829:845	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	5	6	theme	essential	1034:1042	arg1	mannose					1060:1066	mannose	1060:1066	mannose	1060:1066	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	5	6	theme	essential	1034:1042	arg1	components					1044:1053	the essential components	1030:1053	the essential components	1030:1053	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	8	7	theme	molecular	1634:1642	arg1	changes					1654:1660	molecular structure changes	1634:1660	molecular structure changes	1634:1660	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	7	8	dep	contents	1419:1426	arg1	the					1412:1414	the	1412:1414	the	1412:1414	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	6	9	theme	acid	1296:1299	arg1	presence					1277:1284	the presence	1273:1284	the presence of uronic acid and acetyl groups	1273:1317	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	6	10	theme	NMR	1237:1239	arg1	analysis					1241:1248	FT-IR and NMR analysis	1227:1248	analysis	1241:1248	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	2	11	theme	pharmacological	433:447	arg1	due					519:521	due	519:521	due	519:521	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	11	theme	pharmacological	433:447	arg1	activity					449:456	the improved pharmacological activity	420:456	the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS)	420:508	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	6	12	theme	uronic	1289:1294	arg1	acid					1296:1299	uronic acid	1289:1299	uronic acid	1289:1299	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	7	13	theme	IFN-γ	1450:1454	arg1	contents					1419:1426	NO contents	1416:1426	NO contents	1416:1426	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	13	theme	IFN-γ	1450:1454	arg1	expression					1436:1445	the expression	1432:1445	the expression of IFN-γ, IL-1β, IL-22 and TNF-α	1432:1478	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	3	14	theme	scattering	745:754	arg1	detection					756:764	evaporative light scattering detection	727:764	evaporative light scattering detection (HPGFC-ELSD)	727:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	3	14	theme	scattering	745:754	arg1	HPGFC-ELSD					767:776	HPGFC-ELSD	767:776	HPGFC-ELSD	767:776	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	0	15	theme	Structure	0:8	arg1	Characterization					10:25	Structure Characterization	0:25	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.	0:116	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	2	16	theme	polysaccharide	488:501	arg1	due					519:521	due	519:521	due	519:521	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	16	theme	polysaccharide	488:501	arg1	activity					449:456	the improved pharmacological activity	420:456	the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS)	420:508	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	8	17	theme	structure	1644:1652	arg1	changes					1654:1660	molecular structure changes	1634:1660	molecular structure changes	1634:1660	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	4	18	theme	pre-column	937:946	arg1	derivatization					948:961	pre-column derivatization	937:961	pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP)	937:1003	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	2	19	theme	structural	526:535	arg1	changes					537:543	structural changes	526:543	structural changes during processing	526:561	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	3	20	theme	APS	598:600	arg1	weights					578:584	The molecular weights	564:584	The molecular weights of HAPS and APS	564:600	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	3	20	theme	APS	598:600	arg1	Da					617:618	1,695,788 Da	607:618	1,695,788 Da	607:618	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	2	21	theme	Astragalus	477:486	arg1	HAPS					504:507	HAPS	504:507	HAPS	504:507	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	21	theme	Astragalus	477:486	arg1	polysaccharide					488:501	honey-processed Astragalus polysaccharide	461:501	honey-processed Astragalus polysaccharide (HAPS)	461:508	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	22	theme	immunomodulatory	332:347	arg1	effects					349:355	its immunomodulatory effects	328:355	its immunomodulatory effects	328:355	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	23	theme	Astragalus	397:406	arg1	effect					381:386	the tonic effect	371:386	the tonic effect of Radix Astragalus	371:406	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	0	24	theme	Honey-Processed	30:44	arg1	Polysaccharides					57:71	Honey-Processed Astragalus Polysaccharides	30:71	Honey-Processed Astragalus Polysaccharides	30:71	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	6	25	theme	groups	1312:1317	arg1	presence					1277:1284	the presence	1273:1284	the presence of uronic acid and acetyl groups	1273:1317	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	3	26	theme	HAPS	589:592	arg1	weights					578:584	The molecular weights	564:584	The molecular weights of HAPS and APS	564:600	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	3	26	theme	HAPS	589:592	arg1	Da					617:618	1,695,788 Da	607:618	1,695,788 Da	607:618	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	7	27	theme	TNF-α	1474:1478	arg1	contents					1419:1426	NO contents	1416:1426	NO contents	1416:1426	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	27	theme	TNF-α	1474:1478	arg1	expression					1436:1445	the expression	1432:1445	the expression of IFN-γ, IL-1β, IL-22 and TNF-α	1432:1478	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	1	28	theme	Chinese	216:222	arg1	medicine					224:231	a traditional Chinese medicine	202:231	a traditional Chinese medicine processing method	202:249	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	29	theme	Honey-processed	118:132	arg1	form					157:160	a dosage form	148:160	a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect	148:284	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	29	theme	Honey-processed	118:132	arg1	Astragalus					134:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	7	30	theme	-induced	1507:1514	arg1	cells					1525:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	5	31	theme	0.06:28.34:0.58:0.24:0.33:0.21	1146:1175	arg1	ratios					1136:1141	molar ratios	1130:1141	molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56	1130:1210	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	6	32	theme	results	1258:1264	arg1	analysis					1241:1248	FT-IR and NMR analysis	1227:1248	analysis	1241:1248	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	1	33	theme	medicine	224:231	arg1	method					244:249	a traditional Chinese medicine processing method	202:249	a traditional Chinese medicine processing method	202:249	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	0	34	theme	Polysaccharides	57:71	arg1	Characterization					10:25	Structure Characterization	0:25	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.	0:116	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	3	35	theme	high	666:669	arg1	chromatography					698:711	high performance gel filtration chromatography	666:711	high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD)	666:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	2	36	theme	improved	424:431	arg1	due					519:521	due	519:521	due	519:521	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	36	theme	improved	424:431	arg1	activity					449:456	the improved pharmacological activity	420:456	the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS)	420:508	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	4	37	theme	mass	893:896	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	37	theme	mass	893:896	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	1	38	theme	traditional	204:214	arg1	medicine					224:231	a traditional Chinese medicine	202:231	a traditional Chinese medicine processing method	202:249	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	39	theme	processing	233:242	arg1	method					244:249	a traditional Chinese medicine processing method	202:249	a traditional Chinese medicine processing method	202:249	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	0	40	theme	Astragalus	46:55	arg1	Polysaccharides					57:71	Honey-Processed Astragalus Polysaccharides	30:71	Honey-Processed Astragalus Polysaccharides	30:71	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	5	41	theme	0.27:12.83:1.63:0.71:1.04:0.56	1181:1210	arg1	ratios					1136:1141	molar ratios	1130:1141	molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56	1130:1210	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	2	42	theme	honey-processed	461:475	arg1	HAPS					504:507	HAPS	504:507	HAPS	504:507	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	42	theme	honey-processed	461:475	arg1	polysaccharide					488:501	honey-processed Astragalus polysaccharide	461:501	honey-processed Astragalus polysaccharide (HAPS)	461:508	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	8	43	dep	potential	1678:1686	arg1	immunomodulatory					1688:1703	immunomodulatory	1688:1703	immunomodulatory	1688:1703	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	8	44	theme	anti-inflammatory	1563:1579	arg1	improvement					1597:1607	the anti-inflammatory and bioactivity improvement	1559:1607	the anti-inflammatory and bioactivity improvement	1559:1607	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	8	45	theme	potential	1678:1686	arg1	action					1705:1710	the potential immunomodulatory action	1674:1710	the potential immunomodulatory action	1674:1710	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	7	46	theme	IL-1β	1457:1461	arg1	contents					1419:1426	NO contents	1416:1426	NO contents	1416:1426	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	46	theme	IL-1β	1457:1461	arg1	expression					1436:1445	the expression	1432:1445	the expression of IFN-γ, IL-1β, IL-22 and TNF-α	1432:1478	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	47	theme	HAPS	1356:1359	arg1	effective					1371:1379	effective	1371:1379	effective	1371:1379	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	47	theme	HAPS	1356:1359	arg1	activities					1342:1351	The anti-inflammatory activities	1320:1351	The anti-inflammatory activities of HAPS	1320:1359	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	48	theme	IL-22	1464:1468	arg1	contents					1419:1426	NO contents	1416:1426	NO contents	1416:1426	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	7	48	theme	IL-22	1464:1468	arg1	expression					1436:1445	the expression	1432:1445	the expression of IFN-γ, IL-1β, IL-22 and TNF-α	1432:1478	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	6	49	theme	HAPS	1253:1256	arg1	results					1258:1264	HAPS results	1253:1264	HAPS results	1253:1264	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	3	50	theme	filtration	687:696	arg1	chromatography					698:711	high performance gel filtration chromatography	666:711	high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD)	666:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	5	51	theme	molar	1130:1134	arg1	ratios					1136:1141	molar ratios	1130:1141	molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56	1130:1210	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	1	52	theme	dosage	150:155	arg1	form					157:160	a dosage form	148:160	a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect	148:284	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	52	theme	dosage	150:155	arg1	Astragalus					134:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	5	53	theme	glucuronic	1097:1106	arg1	acid					1108:1111	glucuronic acid	1097:1111	glucuronic acid	1097:1111	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	7	54	theme	RAW264.7	1516:1523	arg1	cells					1525:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	1	55	theme	Radix	165:169	arg1	Astragalus					171:180	Radix Astragalus	165:180	Radix Astragalus mixed with honey by a traditional Chinese medicine processing method	165:249	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	4	56	theme	liquid	847:852	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	56	theme	liquid	847:852	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	57	theme	time-of-flight	878:891	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	57	theme	time-of-flight	878:891	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	7	58	from	contents	1419:1426	arg1	cells					1525:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	4	59	theme	monosaccharide	784:797	arg1	composition					799:809	The monosaccharide composition	780:809	The monosaccharide composition	780:809	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	8	60	dep	changes	1654:1660	arg1	bearing					1663:1669	bearing	1663:1669	bearing on the potential immunomodulatory action	1663:1710	This findings suggest that the anti-inflammatory and bioactivity improvement might be associated with molecular structure changes, bearing on the potential immunomodulatory action.
29342936	3	61	theme	molecular	568:576	arg1	weights					578:584	The molecular weights	564:584	The molecular weights of HAPS and APS	564:600	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	3	61	theme	molecular	568:576	arg1	Da					617:618	1,695,788 Da	607:618	1,695,788 Da	607:618	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	3	62	theme	performance	671:681	arg1	chromatography					698:711	high performance gel filtration chromatography	666:711	high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD)	666:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	2	63	theme	Astragalus	287:296	arg1	APS					314:316	APS	314:316	APS	314:316	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	2	63	theme	Astragalus	287:296	arg1	polysaccharide					298:311	Astragalus polysaccharide	287:311	Astragalus polysaccharide	287:311	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	5	64	dep	mannose	1060:1066	arg1	ratios					1136:1141	molar ratios	1130:1141	molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56	1130:1210	The results showed that the essential components were mannose, glucose, xylose, arabinose, glucuronic acid and rhamnose, is molar ratios of 0.06:28.34:0.58:0.24:0.33:0.21 and 0.27:12.83:1.63:0.71:1.04:0.56, respectively.
29342936	6	65	theme	acetyl	1305:1310	arg1	groups					1312:1317	acetyl groups	1305:1317	acetyl groups	1305:1317	FT-IR and NMR analysis of HAPS results showed the presence of uronic acid and acetyl groups.
29342936	0	66	theme	Activity	99:106	arg1	Characterization					10:25	Structure Characterization	0:25	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.	0:116	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	3	67	theme	gel	683:685	arg1	chromatography					698:711	high performance gel filtration chromatography	666:711	high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD)	666:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	4	68	theme	quadrupole	867:876	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	68	theme	quadrupole	867:876	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	7	69	theme	NO	1416:1417	arg1	contents					1419:1426	NO contents	1416:1426	NO contents	1416:1426	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	2	70	theme	Radix	391:395	arg1	Astragalus					397:406	Radix Astragalus	391:406	Radix Astragalus	391:406	Astragalus polysaccharide (APS), perform its immunomodulatory effects by relying on the tonic effect of Radix Astragalus, therefore, the improved pharmacological activity of honey-processed Astragalus polysaccharide (HAPS) might be due to structural changes during processing.
29342936	4	71	with	derivatization	948:961	arg1	PMP					1000:1002	PMP	1000:1002	PMP	1000:1002	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	71	with	derivatization	948:961	arg1	1-phenyl-3-methyl-5-pyrazolone					968:997	1-phenyl-3-methyl-5-pyrazolone	968:997	1-phenyl-3-methyl-5-pyrazolone (PMP)	968:1003	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	0	72	theme	Anti-Inflammatory	81:97	arg1	Activity					99:106	Its Anti-Inflammatory Activity	77:106	Its Anti-Inflammatory Activity In Vitro	77:115	Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.
29342936	3	73	theme	evaporative	727:737	arg1	scattering					745:754	evaporative light scattering	727:754	evaporative light scattering detection (HPGFC-ELSD)	727:777	The molecular weights of HAPS and APS were 1,695,788 Da, 2,047,756 Da, respectively, as determined by high performance gel filtration chromatography combined with evaporative light scattering detection (HPGFC-ELSD).
29342936	4	74	theme	chromatogram	854:865	arg1	UPLC/ESI-Q-TOF-MS					912:928	UPLC/ESI-Q-TOF-MS	912:928	UPLC/ESI-Q-TOF-MS	912:928	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	4	74	theme	chromatogram	854:865	arg1	spectrometry					898:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry	829:909	ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS)	829:929	The monosaccharide composition was determined by ultra-performance liquid chromatogram quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP).
29342936	7	75	from	expression	1436:1445	arg1	cells					1525:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	lipopolysaccharide (LPS)-induced RAW264.7 cells	1483:1529	The anti-inflammatory activities of HAPS were more effective than those of APS according to the NO contents and the expression of IFN-γ, IL-1β, IL-22 and TNF-α in lipopolysaccharide (LPS)-induced RAW264.7 cells.
29342936	1	76	theme	Astragalus	171:180	arg1	form					157:160	a dosage form	148:160	a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect	148:284	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	76	theme	Astragalus	171:180	arg1	Astragalus					134:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus	118:143	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
29342936	1	77	theme	tonic	273:277	arg1	effect					279:284	the tonic effect	269:284	the tonic effect	269:284	Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which strengthens the tonic effect.
31756484	2	0	theme	fermentation	407:418	arg1	time					420:423	the suitable fermentation time	394:423	the suitable fermentation time	394:423	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	5	1	theme	chemical	1170:1177	arg1	analysis					1179:1186	chemical analysis	1170:1186	chemical analysis	1170:1186	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	0	2	theme	exopolysaccharides	80:97	arg1	optimization					24:35	optimization	24:35	optimization	24:35	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	0	2	theme	exopolysaccharides	80:97	arg1	activity					68:75	antioxidant activity	56:75	antioxidant activity	56:75	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	0	2	theme	exopolysaccharides	80:97	arg1	purification					38:49	purification	38:49	purification	38:49	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	6	3	dep	in	1330:1331	arg1	vitro					1333:1337	vitro	1333:1337	vitro	1333:1337	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	2	4	theme	suitable	398:405	arg1	time					420:423	the suitable fermentation time	394:423	the suitable fermentation time	394:423	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	7	5	theme	functional	1498:1507	arg1	studies					1509:1515	functional studies	1498:1515	functional studies	1498:1515	However, further structure elucidation and functional studies need to be continued.
31756484	4	6	theme	culture	896:902	arg1	temperature					904:914	culture temperature 28 °C	896:920	culture temperature 28 °C	896:920	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	7	7	theme	structure	1472:1480	arg1	elucidation					1482:1492	further structure elucidation	1464:1492	further structure elucidation	1464:1492	However, further structure elucidation and functional studies need to be continued.
31756484	2	8	theme	variable	553:560	arg1	method					572:577	the one variable at a time method	545:577	the one variable at a time method (OVAT)	545:584	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	2	8	theme	variable	553:560	arg1	OVAT					580:583	OVAT	580:583	OVAT	580:583	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	4	9	theme	1.41 g/L	872:879	arg1	MnCl2					881:885	1.41 g/L MnCl2	872:885	1.41 g/L MnCl2	872:885	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	10	theme	optimal	709:715	arg1	medium					717:722	The optimal medium	705:722	The optimal medium	705:722	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	11	theme	EPSs	976:979	arg1	yield					967:971	the yield	963:971	the yield of EPSs to 2.89 g/L	963:991	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	0	12	theme	growth-promoting	123:138	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	4	13	from	broth	762:766	arg1	temperature					904:914	culture temperature 28 °C	896:920	culture temperature 28 °C	896:920	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	extract					839:845	9.88 g/L malt extract	825:845	9.88 g/L malt extract	825:845	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	pH					888:889	pH 7.5	888:893	pH 7.5	888:893	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	maltose					816:822	3.65 g/L maltose	807:822	3.65 g/L maltose	807:822	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	200 rpm					927:933	200 rpm	927:933	200 rpm for 7 days	927:944	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	extract					863:869	3.40 g/L yeast extract	848:869	3.40 g/L yeast extract	848:869	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	flasks					789:794	250 mL Erlenmeyer flasks	771:794	250 mL Erlenmeyer flasks	771:794	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	13	from	broth	762:766	arg1	MnCl2					881:885	1.41 g/L MnCl2	872:885	1.41 g/L MnCl2	872:885	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	temperature					904:914	culture temperature 28 °C	896:920	culture temperature 28 °C	896:920	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	extract					839:845	9.88 g/L malt extract	825:845	9.88 g/L malt extract	825:845	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	pH					888:889	pH 7.5	888:893	pH 7.5	888:893	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	maltose					816:822	3.65 g/L maltose	807:822	3.65 g/L maltose	807:822	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	200 rpm					927:933	200 rpm	927:933	200 rpm for 7 days	927:944	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	extract					863:869	3.40 g/L yeast extract	848:869	3.40 g/L yeast extract	848:869	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	flasks					789:794	250 mL Erlenmeyer flasks	771:794	250 mL Erlenmeyer flasks	771:794	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	14	theme	Erlenmeyer	778:787	arg1	MnCl2					881:885	1.41 g/L MnCl2	872:885	1.41 g/L MnCl2	872:885	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	0	15	theme	plant	117:121	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	6	16	theme	radicals	1295:1302	arg1	ability					1231:1237	scavenging ability	1220:1237	scavenging ability	1220:1237	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	6	16	theme	radicals	1295:1302	arg1	power					1252:1256	reducing power	1243:1256	reducing power	1243:1256	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	6	17	theme	anion	1276:1280	arg1	ability					1231:1237	scavenging ability	1220:1237	scavenging ability	1220:1237	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	6	17	theme	anion	1276:1280	arg1	power					1252:1256	reducing power	1243:1256	reducing power	1243:1256	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	4	18	theme	250 mL	771:776	arg1	temperature					904:914	culture temperature 28 °C	896:920	culture temperature 28 °C	896:920	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	extract					839:845	9.88 g/L malt extract	825:845	9.88 g/L malt extract	825:845	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	pH					888:889	pH 7.5	888:893	pH 7.5	888:893	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	maltose					816:822	3.65 g/L maltose	807:822	3.65 g/L maltose	807:822	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	200 rpm					927:933	200 rpm	927:933	200 rpm for 7 days	927:944	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	extract					863:869	3.40 g/L yeast extract	848:869	3.40 g/L yeast extract	848:869	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	flasks					789:794	250 mL Erlenmeyer flasks	771:794	250 mL Erlenmeyer flasks	771:794	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	18	theme	250 mL	771:776	arg1	MnCl2					881:885	1.41 g/L MnCl2	872:885	1.41 g/L MnCl2	872:885	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	2	19	theme	inoculation	439:449	arg1	volume					451:456	inoculation volume	439:456	inoculation volume	439:456	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	6	20	dep	ability	1231:1237	arg1	The					1216:1218	The	1216:1218	The	1216:1218	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	4	21	theme	yeast	857:861	arg1	extract					863:869	3.40 g/L yeast extract	848:869	3.40 g/L yeast extract	848:869	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	22	dep	were	738:741	arg1	broth					762:766	100 mL broth	755:766	100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L	755:991	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	2	23	theme	carbon	477:482	arg1	sources					497:503	the carbon and nitrogen sources	473:503	sources	497:503	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	4	24	theme	3.40 g/L	848:855	arg1	extract					863:869	3.40 g/L yeast extract	848:869	3.40 g/L yeast extract	848:869	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	5	25	theme	purified	998:1005	arg1	5180EPS-1					1013:1021	5180EPS-1	1013:1021	5180EPS-1 (MW 58.9 kDa)	1013:1035	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	5	25	theme	purified	998:1005	arg1	EPSs					1007:1010	Two purified EPSs	994:1010	Two purified EPSs	994:1010	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	5	25	theme	purified	998:1005	arg1	5180EPS-2					1041:1049	5180EPS-2	1041:1049	5180EPS-2 (10.5 kDa)	1041:1060	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	6	26	theme	antioxidants	1441:1452	arg1	source					1423:1428	a new source	1417:1428	a new source of natural antioxidants	1417:1452	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	7	27	theme	further	1464:1470	arg1	elucidation					1482:1492	further structure elucidation	1464:1492	further structure elucidation	1464:1492	However, further structure elucidation and functional studies need to be continued.
31756484	1	28	theme	antioxidant	336:346	arg1	activity					348:355	antioxidant activity	336:355	antioxidant activity	336:355	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	0	29	theme	actinobacterium	151:165	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	4	30	theme	9.88 g/L	825:832	arg1	extract					839:845	9.88 g/L malt extract	825:845	9.88 g/L malt extract	825:845	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	4	31	theme	100 mL	755:760	arg1	broth					762:766	100 mL broth	755:766	100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L	755:991	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	6	32	theme	natural	1433:1439	arg1	antioxidants					1441:1452	natural antioxidants	1433:1452	natural antioxidants	1433:1452	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	1	33	theme	endophytic	227:236	arg1	5180					290:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	5	34	theme	glucuronic	1112:1121	arg1	acid					1123:1126	glucuronic acid	1112:1126	glucuronic acid	1112:1126	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	0	35	theme	endophytic	140:149	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	3	36	used	used	626:629	arg2	design					615:620	a central composition design	593:620	a central composition design	593:620	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	1	37	theme	actinobacterium	238:252	arg1	5180					290:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	2	38	theme	EPSs	509:512	arg1	production					514:523	EPSs production	509:523	EPSs production	509:523	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	0	39	theme	halophytocola	183:195	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	1	40	theme	Glutamicibacter	254:268	arg1	5180					290:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	3	41	theme	central	595:601	arg1	design					615:620	a central composition design	593:620	a central composition design	593:620	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	2	42	theme	pH	459:460	arg1	value					462:466	pH value	459:466	pH value	459:466	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	6	43	theme	new	1419:1421	arg1	source					1423:1428	a new source	1417:1428	a new source of natural antioxidants	1417:1452	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	1	44	theme	exopolysaccharides	360:377	arg1	activity					348:355	antioxidant activity	336:355	antioxidant activity	336:355	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	1	44	theme	exopolysaccharides	360:377	arg1	production					321:330	production	321:330	production	321:330	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	0	45	theme	Glutamicibacter	167:181	arg1	KLBMP					197:201	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	113:206	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	3	46	theme	fermentation	635:646	arg1	optimization					659:670	fermentation conditions optimization	635:670	fermentation conditions optimization	635:670	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	3	47	theme	composition	603:613	arg1	design					615:620	a central composition design	593:620	a central composition design	593:620	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	1	48	theme	halophytocola	270:282	arg1	5180					290:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	0	49	theme	antioxidant	56:66	arg1	activity					68:75	antioxidant activity	56:75	antioxidant activity	56:75	Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180.
31756484	5	50	theme	galacturonic	1084:1095	arg1	acid					1097:1100	galacturonic acid	1084:1100	galacturonic acid	1084:1100	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	6	51	theme	reducing	1243:1250	arg1	power					1252:1256	reducing power	1243:1256	reducing power	1243:1256	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	3	52	theme	conditions	648:657	arg1	optimization					659:670	fermentation conditions optimization	635:670	fermentation conditions optimization	635:670	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	4	53	theme	malt	834:837	arg1	extract					839:845	9.88 g/L malt extract	825:845	9.88 g/L malt extract	825:845	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	6	54	theme	EPSs	1373:1376	arg1	activities					1351:1360	the moderate in vitro antioxidant activities	1317:1360	the moderate in vitro antioxidant activities of the two EPSs	1317:1376	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	2	55	theme	nitrogen	488:495	arg1	sources					497:503	the carbon and nitrogen sources	473:503	sources	497:503	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	6	56	theme	antioxidant	1339:1349	arg1	activities					1351:1360	the moderate in vitro antioxidant activities	1317:1360	the moderate in vitro antioxidant activities of the two EPSs	1317:1376	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	6	57	theme	superoxide	1265:1274	arg1	anion					1276:1280	the superoxide anion and hydroxyl radicals	1261:1302	anion	1276:1280	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	3	58	theme	maximum	686:692	arg1	yield					698:702	the maximum EPS yield	682:702	the maximum EPS yield	682:702	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	3	59	theme	EPS	694:696	arg1	yield					698:702	the maximum EPS yield	682:702	the maximum EPS yield	682:702	Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield.
31756484	5	60	theme	antioxidant	1192:1202	arg1	evaluation					1204:1213	antioxidant evaluation	1192:1213	antioxidant evaluation	1192:1213	Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation.
31756484	1	61	dep	production	321:330	arg1	the					317:319	the	317:319	the	317:319	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	1	62	theme	KLBMP	284:288	arg1	5180					290:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180	224:293	In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs).
31756484	6	63	theme	scavenging	1220:1229	arg1	ability					1231:1237	scavenging ability	1220:1237	scavenging ability	1220:1237	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	6	64	theme	in	1330:1331	arg1	activities					1351:1360	the moderate in vitro antioxidant activities	1317:1360	the moderate in vitro antioxidant activities of the two EPSs	1317:1376	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	4	65	theme	3.65 g/L	807:814	arg1	maltose					816:822	3.65 g/L maltose	807:822	3.65 g/L maltose	807:822	The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L.
31756484	6	66	theme	moderate	1321:1328	arg1	activities					1351:1360	the moderate in vitro antioxidant activities	1317:1360	the moderate in vitro antioxidant activities of the two EPSs	1317:1376	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
31756484	2	67	from	time	567:570	arg1	variable					553:560	variable	553:560	variable	553:560	First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT).
31756484	6	68	theme	hydroxyl	1286:1293	arg1	radicals					1295:1302	the superoxide anion and hydroxyl radicals	1261:1302	radicals	1295:1302	The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants.
30840861	2	0	theme	Young	470:474	arg1	moduli					476:481	their Young moduli	464:481	their Young moduli	464:481	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	0	theme	Young	470:474	arg1	e.g.					484:487	e.g.	484:487	e.g.	484:487	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	1	1	theme	different	319:327	arg1	media					342:346	different cell growing media	319:346	different cell growing media	319:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	4	2	theme	HA	774:775	arg1	composites					790:799	HA:HNTs cryogel composites	774:799	HA:HNTs cryogel composites	774:799	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	3	theme	cells	930:934	arg1	proliferation					854:866	the proliferation	850:866	the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116)	850:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	3	4	theme	clotting	732:739	arg1	index					741:745	a blood clotting index	724:745	a blood clotting index of 17.3 ± 4.8	724:759	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	3	5	theme	hemolysis	634:642	arg1	ratio					644:648	1.37 ± 0.11% hemolysis ratio	621:648	1.37 ± 0.11% hemolysis ratio	621:648	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	1	6	theme	cell	329:332	arg1	media					342:346	different cell growing media	319:346	different cell growing media	319:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	2	7	theme	1:2	545:547	arg1	ratio					536:540	a HA:HNTs weight ratio	519:540	a HA:HNTs weight ratio of 1:2	519:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	1	8	theme	biocompatible	160:172	arg1	composites					182:191	mechanically strong and biocompatible cryogel composites	136:191	mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media	136:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	5	9	theme	proliferation	1096:1108	arg1	increase					1080:1087	an increase	1077:1087	an increase of MSC proliferation	1077:1108	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	4	10	theme	cancer	960:965	arg1	HCT116					974:979	HCT116	974:979	HCT116	974:979	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	10	theme	cancer	960:965	arg1	cells					967:971	human colon cancer cells	948:971	human colon cancer cells (HCT116)	948:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	1	11	theme	growing	334:340	arg1	media					342:346	different cell growing media	319:346	different cell growing media	319:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	4	12	theme	HNTs	777:780	arg1	composites					790:799	HA:HNTs cryogel composites	774:799	HA:HNTs cryogel composites	774:799	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	13	theme	mesenchymal	875:885	arg1	MSC					899:901	MSC	899:901	MSC	899:901	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	13	theme	mesenchymal	875:885	arg1	cells					892:896	rat mesenchymal stem cells	871:896	rat mesenchymal stem cells (MSC)	871:902	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	0	14	theme	tissue	85:90	arg1	engineering					92:102	tissue engineering	85:102	tissue engineering	85:102	Cryogel composites based on hyaluronic acid and halloysite nanotubes as scaffold for tissue engineering.
30840861	3	15	theme	blood	726:730	arg1	index					741:745	a blood clotting index	724:745	a blood clotting index of 17.3 ± 4.8	724:759	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	5	16	theme	increased	1017:1025	arg1	amount					1027:1032	an increased amount	1014:1032	an increased amount of HNT embedding into HA cryogels	1014:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	5	16	theme	increased	1017:1025	arg1	embedding					1041:1049	HNT embedding	1037:1049	HNT embedding into HA cryogels	1037:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	3	17	theme	HA	655:656	arg1	ratio					670:674	a HA:HNTs weight ratio	653:674	a HA:HNTs weight ratio of 1:2	653:681	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	5	18	theme	MSC	1092:1094	arg1	proliferation					1096:1108	MSC proliferation	1092:1108	MSC proliferation	1092:1108	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	1	19	theme	cryogel	174:180	arg1	composites					182:191	mechanically strong and biocompatible cryogel composites	136:191	mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media	136:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	4	20	from	materials	837:845	arg1	proliferation					854:866	the proliferation	850:866	the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116)	850:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	5	21	theme	embedding	1041:1049	arg1	amount					1027:1032	an increased amount	1014:1032	an increased amount of HNT embedding into HA cryogels	1014:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	5	21	theme	embedding	1041:1049	arg1	embedding					1041:1049	HNT embedding	1037:1049	HNT embedding into HA cryogels	1037:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	4	22	theme	cryogel	782:788	arg1	composites					790:799	HA:HNTs cryogel composites	774:799	HA:HNTs cryogel composites	774:799	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	1	23	theme	composites	182:191	arg1	preparation					121:131	preparation	121:131	preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media	121:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	4	24	theme	human	905:909	arg1	HeLa					937:940	HeLa	937:940	HeLa	937:940	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	24	theme	human	905:909	arg1	cells					930:934	human cervical carcinoma cells	905:934	human cervical carcinoma cells (HeLa)	905:941	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	2	25	from	increase	452:459	arg1	moduli					476:481	their Young moduli	464:481	their Young moduli	464:481	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	25	from	increase	452:459	arg1	e.g.					484:487	e.g.	484:487	e.g.	484:487	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	25	from	increase	452:459	arg1	38 ± 1					495:500	38 ± 1	495:500	38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2	495:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	3	26	theme	weight	663:668	arg1	ratio					670:674	a HA:HNTs weight ratio	653:674	a HA:HNTs weight ratio of 1:2	653:681	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	3	27	with	compatible	605:614	arg1	ratio					644:648	1.37 ± 0.11% hemolysis ratio	621:648	1.37 ± 0.11% hemolysis ratio	621:648	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	0	28	theme	Cryogel	0:6	arg1	composites					8:17	Cryogel composites	0:17	Cryogel composites based on hyaluronic acid and halloysite	0:57	Cryogel composites based on hyaluronic acid and halloysite nanotubes as scaffold for tissue engineering.
30840861	1	29	theme	various	258:264	arg1	compositions					266:277	various compositions	258:277	various compositions	258:277	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	3	30	from	ratio	670:674	arg1	compatible					605:614	compatible	605:614	compatible	605:614	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	4	31	theme	cells	967:971	arg1	proliferation					854:866	the proliferation	850:866	the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116)	850:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	32	theme	carcinoma	920:928	arg1	HeLa					937:940	HeLa	937:940	HeLa	937:940	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	32	theme	carcinoma	920:928	arg1	cells					930:934	human cervical carcinoma cells	905:934	human cervical carcinoma cells (HeLa)	905:941	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	1	33	theme	compositions	266:277	arg1	acid					213:216	hyaluronic acid	202:216	hyaluronic acid (HA)	202:221	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	33	theme	compositions	266:277	arg1	HA					219:220	HA	219:220	HA	219:220	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	33	theme	compositions	266:277	arg1	nanotubes					238:246	halloysite nanotubes	227:246	halloysite nanotubes (HNTs) of various compositions	227:277	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	33	theme	compositions	266:277	arg1	HNTs					249:252	HNTs	249:252	HNTs	249:252	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	33	theme	compositions	266:277	arg1	applications					290:301	their applications	284:301	their applications as scaffold for different cell growing media	284:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	4	34	theme	stem	887:890	arg1	MSC					899:901	MSC	899:901	MSC	899:901	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	34	theme	stem	887:890	arg1	cells					892:896	rat mesenchymal stem cells	871:896	rat mesenchymal stem cells (MSC)	871:902	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	1	35	theme	hyaluronic	202:211	arg1	acid					213:216	hyaluronic acid	202:216	hyaluronic acid (HA)	202:221	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	35	theme	hyaluronic	202:211	arg1	HA					219:220	HA	219:220	HA	219:220	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	2	36	theme	HA	419:420	arg1	cryogels					422:429	HA cryogels	419:429	HA cryogels	419:429	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	4	37	theme	excellent	818:826	arg1	materials					837:845	excellent scaffold materials	818:845	excellent scaffold materials	818:845	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	2	38	theme	HNTs	409:412	arg1	incorporation					392:404	the incorporation	388:404	the incorporation of HNTs into HA cryogels	388:429	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	5	39	theme	HNT	1037:1039	arg1	embedding					1041:1049	HNT embedding	1037:1049	HNT embedding into HA cryogels	1037:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	3	40	theme	HNTs	658:661	arg1	ratio					670:674	a HA:HNTs weight ratio	653:674	a HA:HNTs weight ratio of 1:2	653:681	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	4	41	theme	scaffold	828:835	arg1	materials					837:845	excellent scaffold materials	818:845	excellent scaffold materials	818:845	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	42	theme	human	948:952	arg1	HCT116					974:979	HCT116	974:979	HCT116	974:979	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	42	theme	human	948:952	arg1	cells					967:971	human colon cancer cells	948:971	human colon cancer cells (HCT116)	948:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	0	43	theme	hyaluronic	28:37	arg1	acid					39:42	hyaluronic acid	28:42	hyaluronic acid	28:42	Cryogel composites based on hyaluronic acid and halloysite nanotubes as scaffold for tissue engineering.
30840861	4	44	theme	cells	892:896	arg1	proliferation					854:866	the proliferation	850:866	the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116)	850:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	3	45	theme	based	567:571	arg1	cryogels					573:580	HA:HNTs based cryogels	559:580	HA:HNTs based cryogels	559:580	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	2	46	theme	~2.5-fold	442:450	arg1	increase					452:459	a ~2.5-fold increase	440:459	a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2	440:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	47	theme	weight	529:534	arg1	ratio					536:540	a HA:HNTs weight ratio	519:540	a HA:HNTs weight ratio of 1:2	519:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	48	from	ratio	536:540	arg1	99 ± 4 kPa					505:514	99 ± 4 kPa	505:514	99 ± 4 kPa at a HA:HNTs weight ratio of 1:2	505:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	3	49	theme	1.37 ± 0.11	621:631	arg1	%					632:632	%	632:632	%	632:632	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	2	50	theme	HNTs	524:527	arg1	ratio					536:540	a HA:HNTs weight ratio	519:540	a HA:HNTs weight ratio of 1:2	519:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	3	51	theme	1:2	679:681	arg1	ratio					670:674	a HA:HNTs weight ratio	653:674	a HA:HNTs weight ratio of 1:2	653:681	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	4	52	theme	cervical	911:918	arg1	HeLa					937:940	HeLa	937:940	HeLa	937:940	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	52	theme	cervical	911:918	arg1	cells					930:934	human cervical carcinoma cells	905:934	human cervical carcinoma cells (HeLa)	905:941	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	2	53	theme	HA	521:522	arg1	ratio					536:540	a HA:HNTs weight ratio	519:540	a HA:HNTs weight ratio of 1:2	519:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	54	from	38 ± 1	495:500	arg1	moduli					476:481	their Young moduli	464:481	their Young moduli	464:481	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	54	from	38 ± 1	495:500	arg1	increase					452:459	a ~2.5-fold increase	440:459	a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2	440:547	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	2	54	from	38 ± 1	495:500	arg1	e.g.					484:487	e.g.	484:487	e.g.	484:487	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	3	55	theme	HNTs	562:565	arg1	cryogels					573:580	HA:HNTs based cryogels	559:580	HA:HNTs based cryogels	559:580	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	2	56	theme	Uniaxial	349:356	arg1	tests					370:374	Uniaxial compression tests	349:374	Uniaxial compression tests	349:374	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	4	57	theme	colon	954:958	arg1	HCT116					974:979	HCT116	974:979	HCT116	974:979	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	57	theme	colon	954:958	arg1	cells					967:971	human colon cancer cells	948:971	human colon cancer cells (HCT116)	948:980	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	58	theme	rat	871:873	arg1	MSC					899:901	MSC	899:901	MSC	899:901	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	4	58	theme	rat	871:873	arg1	cells					892:896	rat mesenchymal stem cells	871:896	rat mesenchymal stem cells (MSC)	871:902	Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116).
30840861	5	59	theme	cell	987:990	arg1	studies					992:998	The cell studies	983:998	The cell studies	983:998	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	2	60	theme	compression	358:368	arg1	tests					370:374	Uniaxial compression tests	349:374	Uniaxial compression tests	349:374	Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2.
30840861	3	61	theme	17.3 ± 4.8	750:759	arg1	index					741:745	a blood clotting index	724:745	a blood clotting index of 17.3 ± 4.8	724:759	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	1	62	theme	halloysite	227:236	arg1	HNTs					249:252	HNTs	249:252	HNTs	249:252	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	1	62	theme	halloysite	227:236	arg1	nanotubes					238:246	halloysite nanotubes	227:246	halloysite nanotubes (HNTs) of various compositions	227:277	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	5	63	theme	HA	1056:1057	arg1	cryogels					1059:1066	HA cryogels	1056:1066	HA cryogels	1056:1066	The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
30840861	3	64	theme	%	632:632	arg1	ratio					644:648	1.37 ± 0.11% hemolysis ratio	621:648	1.37 ± 0.11% hemolysis ratio	621:648	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	3	65	theme	HA	559:560	arg1	cryogels					573:580	HA:HNTs based cryogels	559:580	HA:HNTs based cryogels	559:580	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
30840861	1	66	theme	strong	149:154	arg1	composites					182:191	mechanically strong and biocompatible cryogel composites	136:191	mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media	136:346	We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media.
30840861	3	67	theme	thrombogenic	697:708	arg1	activity					710:717	thrombogenic activity	697:717	thrombogenic activity	697:717	Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8.
29578013	4	0	from	effects	467:473	arg1	concentrations					508:521	different bath concentrations	493:521	different bath concentrations	493:521	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	0	from	effects	467:473	arg1	crystallinity					554:566	crystallinity	554:566	crystallinity	554:566	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	0	from	effects	467:473	arg1	morphology					573:582	morphology	573:582	morphology	573:582	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	0	from	effects	467:473	arg1	performance					541:551	mechanical performance	530:551	mechanical performance	530:551	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	7	1	theme	grooves	920:926	arg1	presence					908:915	the presence	904:915	the presence of grooves on the fiber surface	904:947	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	2	2	theme	7wt	339:341	arg1	NaOH					344:347	7wt% NaOH	339:347	7wt% NaOH	339:347	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	3	3	contain	containing	434:443	arg1	bath					429:432	a coagulation bath	415:432	a coagulation bath containing H2SO4 and Na2SO4	415:460	The fiber was stretched in a coagulation bath containing H2SO4 and Na2SO4.
29578013	3	3	contain	containing	434:443	arg2	Na2SO4					455:460	Na2SO4	455:460	Na2SO4	455:460	The fiber was stretched in a coagulation bath containing H2SO4 and Na2SO4.
29578013	3	3	contain	containing	434:443	arg2	H2SO4					445:449	H2SO4	445:449	H2SO4	445:449	The fiber was stretched in a coagulation bath containing H2SO4 and Na2SO4.
29578013	0	4	theme	coagulation	87:97	arg1	baths					99:103	different coagulation baths	77:103	different coagulation baths	77:103	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	7	5	from	presence	908:915	arg1	surface					941:947	the fiber surface	931:947	the fiber surface	931:947	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	9	6	dep	results	1117:1123	arg1	basis					1095:1099	basis	1095:1099	basis	1095:1099	On the basis of the obtained results, we finally optimized the coagulation bath composition and concentration.
29578013	9	6	dep	results	1117:1123	arg1	the					1091:1093	the	1091:1093	the	1091:1093	On the basis of the obtained results, we finally optimized the coagulation bath composition and concentration.
29578013	4	7	theme	mechanical	530:539	arg1	performance					541:551	mechanical performance	530:551	mechanical performance	530:551	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	8	8	from	percentage	963:972	arg1	fibers					1015:1020	the C/AKP composite fibers	995:1020	the C/AKP composite fibers	995:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	2	9	contain	containing	328:337	arg2	NaOH					344:347	7wt% NaOH	339:347	7wt% NaOH	339:347	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	9	contain	containing	328:337	arg1	solution					319:326	an aqueous solution	308:326	an aqueous solution	308:326	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	9	contain	containing	328:337	arg2	urea					359:362	12wt% urea	353:362	12wt% urea	353:362	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	9	contain	containing	328:337	arg1	eco-friendly					374:385	eco-friendly	374:385	eco-friendly	374:385	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	9	contain	containing	328:337	arg1	solvent					296:302	the solvent	292:302	the solvent	292:302	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	6	10	theme	bath	767:770	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	10	theme	bath	767:770	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	8	11	theme	bonds	986:990	arg1	percentage					963:972	percentage	963:972	percentage	963:972	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	8	11	theme	bonds	986:990	arg1	type					954:957	type	954:957	type	954:957	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	6	12	theme	coagulation	755:765	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	12	theme	coagulation	755:765	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	8	13	dep	type	954:957	arg1	The					950:952	The	950:952	The	950:952	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	5	14	theme	bath	744:747	arg1	concentration					723:735	the concentration	719:735	the concentration of the bath	719:747	The break strength firstly increased and then decreased with an increase in the concentration of the bath.
29578013	1	15	theme	wet	189:191	arg1	spinning					193:200	wet spinning	189:200	wet spinning	189:200	In this paper, cellulose/Antarctic krill protein composite fibers were obtained by wet spinning.
29578013	8	16	dep	transform	1047:1055	arg1	infrared					1057:1064	infrared	1057:1064	transform infrared spectroscopy (FT-IR)	1047:1085	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	8	17	from	type	954:957	arg1	fibers					1015:1020	the C/AKP composite fibers	995:1020	the C/AKP composite fibers	995:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	2	18	theme	12wt	353:356	arg1	urea					359:362	12wt% urea	353:362	12wt% urea	353:362	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	8	19	dep	Fourier	1039:1045	arg1	transform					1047:1055	transform	1047:1055	transform infrared spectroscopy (FT-IR)	1047:1085	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	7	20	theme	microscopy	865:874	arg1	observations					882:893	Scanning electron microscopy (SEM) observations	847:893	Scanning electron microscopy (SEM) observations	847:893	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	6	21	from	concentration	772:784	arg1	crystallinity					821:833	the crystallinity	817:833	the crystallinity	817:833	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	21	from	concentration	772:784	arg1	%					844:844	15.16%	839:844	15.16%	839:844	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	0	22	theme	krill	34:38	arg1	fibers					58:63	cellulose/Antarctic krill protein composite fibers	14:63	cellulose/Antarctic krill protein composite fibers	14:63	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	4	23	theme	bath	503:506	arg1	concentrations					508:521	different bath concentrations	493:521	different bath concentrations	493:521	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	8	24	theme	composite	1005:1013	arg1	fibers					1015:1020	the C/AKP composite fibers	995:1020	the C/AKP composite fibers	995:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	9	25	theme	obtained	1108:1115	arg1	results					1117:1123	the obtained results	1104:1123	the obtained results	1104:1123	On the basis of the obtained results, we finally optimized the coagulation bath composition and concentration.
29578013	4	26	theme	C/AKP	591:595	arg1	fibers					607:612	the C/AKP composite fibers	587:612	the C/AKP composite fibers	587:612	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	0	27	theme	cellulose/Antarctic	14:32	arg1	fibers					58:63	cellulose/Antarctic krill protein composite fibers	14:63	cellulose/Antarctic krill protein composite fibers	14:63	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	7	28	attach	presence	908:915	arg1	surface					941:947	the fiber surface	931:947	the fiber surface	931:947	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	7	28	attach	presence	908:915	arg2	grooves					920:926	grooves	920:926	grooves	920:926	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	8	29	theme	C/AKP	999:1003	arg1	fibers					1015:1020	the C/AKP composite fibers	995:1020	the C/AKP composite fibers	995:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	5	30	from	increase	707:714	arg1	concentration					723:735	the concentration	719:735	the concentration of the bath	719:747	The break strength firstly increased and then decreased with an increase in the concentration of the bath.
29578013	3	31	theme	coagulation	417:427	arg1	bath					429:432	a coagulation bath	415:432	a coagulation bath containing H2SO4 and Na2SO4	415:460	The fiber was stretched in a coagulation bath containing H2SO4 and Na2SO4.
29578013	0	32	theme	composite	48:56	arg1	fibers					58:63	cellulose/Antarctic krill protein composite fibers	14:63	cellulose/Antarctic krill protein composite fibers	14:63	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	2	33	theme	aqueous	311:317	arg1	solution					319:326	an aqueous solution	308:326	an aqueous solution	308:326	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	33	theme	aqueous	311:317	arg1	eco-friendly					374:385	eco-friendly	374:385	eco-friendly	374:385	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	33	theme	aqueous	311:317	arg1	solvent					296:302	the solvent	292:302	the solvent	292:302	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	4	34	theme	fibers	607:612	arg1	crystallinity					554:566	crystallinity	554:566	crystallinity	554:566	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	34	theme	fibers	607:612	arg1	morphology					573:582	morphology	573:582	morphology	573:582	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	34	theme	fibers	607:612	arg1	performance					541:551	mechanical performance	530:551	mechanical performance	530:551	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	0	35	theme	protein	40:46	arg1	fibers					58:63	cellulose/Antarctic krill protein composite fibers	14:63	cellulose/Antarctic krill protein composite fibers	14:63	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	4	36	theme	coagulation	478:488	arg1	effects					467:473	The effects	463:473	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers	463:612	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	9	37	theme	coagulation	1151:1161	arg1	composition					1168:1178	the coagulation bath composition	1147:1178	the coagulation bath composition	1147:1178	On the basis of the obtained results, we finally optimized the coagulation bath composition and concentration.
29578013	4	38	from	concentrations	508:521	arg1	coagulation					478:488	coagulation	478:488	coagulation at different bath concentrations	478:521	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	4	38	from	concentrations	508:521	arg1	effects					467:473	The effects	463:473	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers	463:612	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	8	39	theme	hydrogen	977:984	arg1	bonds					986:990	hydrogen bonds	977:990	hydrogen bonds in the C/AKP composite fibers	977:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	4	40	theme	composite	597:605	arg1	fibers					607:612	the C/AKP composite fibers	587:612	the C/AKP composite fibers	587:612	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	6	41	theme	/12wt	808:812	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	41	theme	/12wt	808:812	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	0	42	theme	fibers	58:63	arg1	Properties					0:9	Properties	0:9	Properties of cellulose/Antarctic krill protein composite fibers	0:63	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	4	43	theme	different	493:501	arg1	concentrations					508:521	different bath concentrations	493:521	different bath concentrations	493:521	The effects of coagulation at different bath concentrations on the mechanical performance, crystallinity, and morphology of the C/AKP composite fibers were studied systematically.
29578013	5	44	theme	break	647:651	arg1	strength					653:660	The break strength	643:660	The break strength	643:660	The break strength firstly increased and then decreased with an increase in the concentration of the bath.
29578013	6	45	theme	%	807:807	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	45	theme	%	807:807	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	2	46	theme	recyclable	226:235	arg1	C					248:248	C	248:248	C	248:248	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	46	theme	recyclable	226:235	arg1	materials					211:219	The raw materials	203:219	The raw materials	203:219	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	46	theme	recyclable	226:235	arg1	cellulose					237:245	recyclable cellulose	226:245	recyclable cellulose (C)	226:249	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	7	47	theme	fiber	935:939	arg1	surface					941:947	the fiber surface	931:947	the fiber surface	931:947	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	1	48	theme	cellulose/Antarctic	121:139	arg1	fibers					165:170	cellulose/Antarctic krill protein composite fibers	121:170	cellulose/Antarctic krill protein composite fibers	121:170	In this paper, cellulose/Antarctic krill protein composite fibers were obtained by wet spinning.
29578013	7	49	theme	Scanning	847:854	arg1	SEM					877:879	SEM	877:879	SEM	877:879	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	7	49	theme	Scanning	847:854	arg1	microscopy					865:874	Scanning electron microscopy	847:874	Scanning electron microscopy (SEM) observations	847:893	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	9	50	theme	bath	1163:1166	arg1	composition					1168:1178	the coagulation bath composition	1147:1178	the coagulation bath composition	1147:1178	On the basis of the obtained results, we finally optimized the coagulation bath composition and concentration.
29578013	6	51	theme	12wt	803:806	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	51	theme	12wt	803:806	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	2	52	theme	krill	265:269	arg1	AKP					280:282	AKP	280:282	AKP	280:282	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	52	theme	krill	265:269	arg1	protein					271:277	Antarctic krill protein	255:277	Antarctic krill protein (AKP)	255:283	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	1	53	theme	krill	141:145	arg1	fibers					165:170	cellulose/Antarctic krill protein composite fibers	121:170	cellulose/Antarctic krill protein composite fibers	121:170	In this paper, cellulose/Antarctic krill protein composite fibers were obtained by wet spinning.
29578013	7	54	theme	electron	856:863	arg1	SEM					877:879	SEM	877:879	SEM	877:879	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	7	54	theme	electron	856:863	arg1	microscopy					865:874	Scanning electron microscopy	847:874	Scanning electron microscopy (SEM) observations	847:893	Scanning electron microscopy (SEM) observations revealed the presence of grooves on the fiber surface.
29578013	2	55	theme	raw	207:209	arg1	materials					211:219	The raw materials	203:219	The raw materials	203:219	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	55	theme	raw	207:209	arg1	cellulose					237:245	recyclable cellulose	226:245	recyclable cellulose (C)	226:249	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	56	theme	Antarctic	255:263	arg1	AKP					280:282	AKP	280:282	AKP	280:282	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	56	theme	Antarctic	255:263	arg1	protein					271:277	Antarctic krill protein	255:277	Antarctic krill protein (AKP)	255:283	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	1	57	theme	protein	147:153	arg1	fibers					165:170	cellulose/Antarctic krill protein composite fibers	121:170	cellulose/Antarctic krill protein composite fibers	121:170	In this paper, cellulose/Antarctic krill protein composite fibers were obtained by wet spinning.
29578013	8	58	from	fibers	1015:1020	arg1	percentage					963:972	percentage	963:972	percentage	963:972	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	8	58	from	fibers	1015:1020	arg1	type					954:957	type	954:957	type	954:957	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
29578013	0	59	theme	different	77:85	arg1	baths					99:103	different coagulation baths	77:103	different coagulation baths	77:103	Properties of cellulose/Antarctic krill protein composite fibers prepared in different coagulation baths.
29578013	6	60	theme	H2SO2/Na2SO4	789:800	arg1	%					813:813	12wt%/12wt%	803:813	12wt%/12wt%	803:813	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	6	60	theme	H2SO2/Na2SO4	789:800	arg1	concentration					772:784	a coagulation bath concentration	753:784	a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%)	753:814	At a coagulation bath concentration of H2SO2/Na2SO4 (12wt%/12wt%), the crystallinity was 15.16%.
29578013	1	61	theme	composite	155:163	arg1	fibers					165:170	cellulose/Antarctic krill protein composite fibers	121:170	cellulose/Antarctic krill protein composite fibers	121:170	In this paper, cellulose/Antarctic krill protein composite fibers were obtained by wet spinning.
29578013	2	62	theme	%	357:357	arg1	urea					359:362	12wt% urea	353:362	12wt% urea	353:362	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	2	63	theme	%	342:342	arg1	NaOH					344:347	7wt% NaOH	339:347	7wt% NaOH	339:347	The raw materials were recyclable cellulose (C) and Antarctic krill protein (AKP), while the solvent was an aqueous solution containing 7wt% NaOH and 12wt% urea, which is eco-friendly.
29578013	8	64	from	bonds	986:990	arg1	fibers					1015:1020	the C/AKP composite fibers	995:1020	the C/AKP composite fibers	995:1020	The type and percentage of hydrogen bonds in the C/AKP composite fibers were analyzed by Fourier transform infrared spectroscopy (FT-IR).
31677836	4	0	theme	moieties	1033:1040	arg1	mass					974:977	the molecular mass	960:977	the molecular mass	960:977	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	4	0	theme	moieties	1033:1040	arg1	sequences					994:1002	glycosylation sequences	980:1002	glycosylation sequences	980:1002	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	4	0	theme	moieties	1033:1040	arg1	structure					1009:1017	structure	1009:1017	structure	1009:1017	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	3	1	theme	ion	756:758	arg1	mode					760:763	negative ion mode	747:763	negative ion mode	747:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	0	2	theme	stress	184:189	arg1	oligosaccharides					90:105	oligosaccharides	90:105	oligosaccharides	90:105	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	2	theme	stress	184:189	arg1	lipids					79:84	ubiquitous minor lipids	62:84	ubiquitous minor lipids	62:84	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	2	theme	stress	184:189	arg1	inhibition					160:169	their inhibition	154:169	their inhibition of oxidative stress in human plasma	154:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	3	theme	C18	363:365	arg1	column					372:377	a water-preconditioned short C18 open column	334:377	a water-preconditioned short C18 open column	334:377	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	5	4	theme	lipopolysaccharide	1115:1132	arg1	combinations					1086:1097	combinations	1086:1097	combinations of one selected lipopolysaccharide	1086:1132	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	0	5	theme	oligosaccharides	90:105	arg1	characterization					42:57	Electrospray ionization mass spectrometry characterization	0:57	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	3	6	theme	chromatography	680:693	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	0	7	theme	oxidative	174:182	arg1	stress					184:189	oxidative stress	174:189	oxidative stress in human plasma	174:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	8	theme	methanol/water	416:429	arg1	gradient					404:411	a gradient	402:411	a gradient of methanol/water	402:429	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	5	9	theme	selected	1106:1113	arg1	lipopolysaccharide					1115:1132	lipopolysaccharide	1115:1132	one selected lipopolysaccharide	1102:1132	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	0	10	from	inhibition	160:169	arg1	plasma					200:205	human plasma	194:205	human plasma	194:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	10	from	inhibition	160:169	arg1	milk					110:113	milk	110:113	milk of the camel (Camelus dromedarius)	110:148	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	3	11	theme	electrospray	695:706	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	0	12	theme	human	194:198	arg1	plasma					200:205	human plasma	194:205	human plasma	194:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	5	13	theme	functional	1234:1243	arg1	study					1252:1256	a density functional theory study	1224:1256	a density functional theory study	1224:1256	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	0	14	dep	camel	122:126	arg1	dromedarius					137:147	Camelus dromedarius	129:147	Camelus dromedarius	129:147	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	15	theme	minor	250:254	arg1	lipids					256:261	minor lipids	250:261	minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water	250:429	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	4	16	theme	glycosylation	980:992	arg1	sequences					994:1002	glycosylation sequences	980:1002	glycosylation sequences	980:1002	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	4	17	theme	revealed	1050:1057	arg1	compounds					1059:1067	the revealed compounds	1046:1067	the revealed compounds	1046:1067	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	0	18	theme	camel	122:126	arg1	milk					110:113	milk	110:113	milk of the camel (Camelus dromedarius)	110:148	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	19	theme	water-preconditioned	336:355	arg1	column					372:377	a water-preconditioned short C18 open column	334:377	a water-preconditioned short C18 open column	334:377	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	4	20	theme	tandem	880:885	arg1	spectrometry					892:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	1	21	from	loading	320:326	arg1	column					372:377	a water-preconditioned short C18 open column	334:377	a water-preconditioned short C18 open column	334:377	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	1	22	theme	short	357:361	arg1	column					372:377	a water-preconditioned short C18 open column	334:377	a water-preconditioned short C18 open column	334:377	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	4	23	theme	ionization	869:878	arg1	spectrometry					892:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	5	24	theme	theory	1245:1250	arg1	study					1252:1256	a density functional theory study	1224:1256	a density functional theory study	1224:1256	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	3	25	from	spectrometry	731:742	arg1	mode					760:763	negative ion mode	747:763	negative ion mode	747:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	0	26	theme	ionization	13:22	arg1	spectrometry					29:40	Electrospray ionization mass spectrometry	0:40	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	27	from	characterization	42:57	arg1	milk					110:113	milk	110:113	milk of the camel (Camelus dromedarius)	110:148	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	2	28	theme	particular	613:622	arg1	polysaccharides					624:638	particular polysaccharides	613:638	particular polysaccharides	613:638	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	3	29	theme	negative	747:754	arg1	mode					760:763	negative ion mode	747:763	negative ion mode	747:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	0	30	theme	Electrospray	0:11	arg1	spectrometry					29:40	Electrospray ionization mass spectrometry	0:40	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	5	31	theme	density	1226:1232	arg1	study					1252:1256	a density functional theory study	1224:1256	a density functional theory study	1224:1256	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	4	32	theme	mass	887:890	arg1	spectrometry					892:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	5	33	theme	combinations	1086:1097	arg1	sequence					1074:1081	The sequence	1070:1081	The sequence	1070:1081	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	6	34	theme	glycosphingolipids	1332:1349	arg1	fraction					1303:1310	the tested fraction	1292:1310	the tested fraction	1292:1310	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	34	theme	glycosphingolipids	1332:1349	arg1	source					1322:1327	a rich source	1315:1327	a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity	1315:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	0	35	theme	spectrometry	29:40	arg1	characterization					42:57	Electrospray ionization mass spectrometry characterization	0:57	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	36	theme	methanol	266:273	arg1	fraction					275:282	methanol fraction	266:282	methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water	266:429	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	4	37	theme	molecular	964:972	arg1	mass					974:977	the molecular mass	960:977	the molecular mass	960:977	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	2	38	theme	fatty	660:664	arg1	acids					666:670	free fatty acids	655:670	free fatty acids	655:670	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	39	theme	free	655:658	arg1	acids					666:670	free fatty acids	655:670	free fatty acids	655:670	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	0	40	theme	mass	24:27	arg1	spectrometry					29:40	Electrospray ionization mass spectrometry	0:40	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	41	from	stress	184:189	arg1	plasma					200:205	human plasma	194:205	human plasma	194:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	42	from	lipids	256:261	arg1	fraction					275:282	methanol fraction	266:282	methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water	266:429	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	1	43	theme	open	367:370	arg1	column					372:377	a water-preconditioned short C18 open column	334:377	a water-preconditioned short C18 open column	334:377	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	2	44	theme	fractionation	459:471	arg1	efficiency					473:482	high fractionation efficiency	454:482	high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides,	454:569	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	1	45	dep	loading	320:326	arg1	it					328:329	it	328:329	it	328:329	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	6	46	theme	antioxidant	1399:1409	arg1	activity					1411:1418	antioxidant activity	1399:1418	antioxidant activity	1399:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	0	47	from	oligosaccharides	90:105	arg1	plasma					200:205	human plasma	194:205	human plasma	194:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	47	from	oligosaccharides	90:105	arg1	milk					110:113	milk	110:113	milk of the camel (Camelus dromedarius)	110:148	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	6	48	theme	tested	1296:1301	arg1	fraction					1303:1310	the tested fraction	1292:1310	the tested fraction	1292:1310	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	48	theme	tested	1296:1301	arg1	source					1322:1327	a rich source	1315:1327	a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity	1315:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	2	49	theme	high	454:457	arg1	efficiency					473:482	high fractionation efficiency	454:482	high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides,	454:569	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	4	50	theme	saccharide	1022:1031	arg1	moieties					1033:1040	saccharide moieties	1022:1040	saccharide moieties	1022:1040	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	3	51	used	used	769:772	arg2	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	6	52	theme	lipopolysaccharides	1352:1370	arg1	fraction					1303:1310	the tested fraction	1292:1310	the tested fraction	1292:1310	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	52	theme	lipopolysaccharides	1352:1370	arg1	source					1322:1327	a rich source	1315:1327	a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity	1315:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	0	53	theme	minor	73:77	arg1	lipids					79:84	ubiquitous minor lipids	62:84	ubiquitous minor lipids	62:84	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	54	theme	raw	299:301	arg1	milk					309:312	raw camel milk	299:312	raw camel milk	299:312	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	6	55	theme	oligosaccharides	1377:1392	arg1	fraction					1303:1310	the tested fraction	1292:1310	the tested fraction	1292:1310	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	55	theme	oligosaccharides	1377:1392	arg1	source					1322:1327	a rich source	1315:1327	a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity	1315:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	0	56	from	lipids	79:84	arg1	plasma					200:205	human plasma	194:205	human plasma	194:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	56	from	lipids	79:84	arg1	milk					110:113	milk	110:113	milk of the camel (Camelus dromedarius)	110:148	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	4	57	theme	Electrospray	856:867	arg1	spectrometry					892:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry	856:903	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	3	58	theme	mass	726:729	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	5	59	theme	lipopolysaccharides	1186:1204	arg1	backbone					1160:1167	the backbone	1156:1167	the backbone of the remaining lipopolysaccharides	1156:1204	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	0	60	theme	ubiquitous	62:71	arg1	lipids					79:84	ubiquitous minor lipids	62:84	ubiquitous minor lipids	62:84	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	1	61	theme	camel	303:307	arg1	milk					309:312	raw camel milk	299:312	raw camel milk	299:312	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	4	62	theme	relevant	930:937	arg1	data					939:942	relevant data	930:942	relevant data	930:942	Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds.
31677836	2	63	from	constituents	596:607	arg1	acids					666:670	free fatty acids	655:670	free fatty acids	655:670	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	63	from	constituents	596:607	arg1	proteins					641:648	proteins	641:648	proteins	641:648	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	63	from	constituents	596:607	arg1	polysaccharides					624:638	particular polysaccharides	613:638	particular polysaccharides	613:638	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	64	theme	other	590:594	arg1	constituents					596:607	other constituents	590:607	other constituents	590:607	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	0	65	from	plasma	200:205	arg1	oligosaccharides					90:105	oligosaccharides	90:105	oligosaccharides	90:105	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	65	from	plasma	200:205	arg1	lipids					79:84	ubiquitous minor lipids	62:84	ubiquitous minor lipids	62:84	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	0	65	from	plasma	200:205	arg1	inhibition					160:169	their inhibition	154:169	their inhibition of oxidative stress in human plasma	154:205	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	3	66	theme	Liquid	673:678	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	6	67	with	source	1322:1327	arg1	activity					1411:1418	antioxidant activity	1399:1418	antioxidant activity	1399:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	0	68	theme	inhibition	160:169	arg1	characterization					42:57	Electrospray ionization mass spectrometry characterization	0:57	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	6	69	theme	rich	1317:1320	arg1	fraction					1303:1310	the tested fraction	1292:1310	the tested fraction	1292:1310	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	69	theme	rich	1317:1320	arg1	source					1322:1327	a rich source	1315:1327	a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity	1315:1418	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	6	70	theme	obtained	1263:1270	arg1	results					1272:1278	The obtained results	1259:1278	The obtained results	1259:1278	The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.
31677836	2	71	theme	minor	487:491	arg1	lipids					493:498	minor lipids	487:498	minor lipids	487:498	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	71	theme	minor	487:491	arg1	lipopolysaccharides					529:547	lipopolysaccharides	529:547	lipopolysaccharides	529:547	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	71	theme	minor	487:491	arg1	oligosaccharides					553:568	oligosaccharides	553:568	oligosaccharides	553:568	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	71	theme	minor	487:491	arg1	glycosphingolipids					509:526	glycosphingolipids	509:526	glycosphingolipids	509:526	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	2	72	theme	lipids	493:498	arg1	efficiency					473:482	high fractionation efficiency	454:482	high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides,	454:569	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	0	73	from	milk	110:113	arg1	characterization					42:57	Electrospray ionization mass spectrometry characterization	0:57	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	5	74	theme	remaining	1176:1184	arg1	lipopolysaccharides					1186:1204	the remaining lipopolysaccharides	1172:1204	the remaining lipopolysaccharides	1172:1204	The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study.
31677836	1	75	theme	study	224:228	arg1	aim					212:214	The aim	208:214	The aim of this study	208:228	The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water.
31677836	0	76	theme	lipids	79:84	arg1	characterization					42:57	Electrospray ionization mass spectrometry characterization	0:57	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.	0:206	Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma.
31677836	3	77	theme	ionization	708:717	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	3	78	theme	tandem	719:724	arg1	spectrometry					731:742	Liquid chromatography electrospray ionization tandem mass spectrometry	673:742	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode	673:763	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
31677836	2	79	theme	C18	436:438	arg1	column					440:445	The C18 column	432:445	The C18 column	432:445	The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids.
31677836	3	80	theme	new	789:791	arg1	glycosphingolipids					793:810	21 new glycosphingolipids	786:810	21 new glycosphingolipids	786:810	Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides.
30756330	2	0	theme	harder	334:339	arg1	woods					341:345	harder woods	334:345	harder woods	334:345	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	1	1	theme	groups	248:253	arg1	richness					267:274	functional feeding groups and species richness	229:274	functional feeding groups and species richness of aquatic insects in a tropical stream	229:314	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	2	2	with	communities	375:385	arg1	profile					435:441	functional food group profile	413:441	functional food group profile	413:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	2	with	communities	375:385	arg1	richness					400:407	richness	400:407	richness	400:407	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	2	with	communities	375:385	arg1	species					392:398	species richness and functional food group profile	392:441	species richness and functional food group profile	392:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	1	3	theme	species	259:265	arg1	richness					267:274	functional feeding groups and species richness	229:274	functional feeding groups and species richness of aquatic insects in a tropical stream	229:314	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	6	4	theme	higher	939:944	arg1	concentrations					946:959	higher concentrations	939:959	higher concentrations of lignin, cellulose, and hemicellulose	939:999	Psychotria grandis shows higher concentrations of lignin, cellulose, and hemicellulose, followed by G. lindeniana and M. leucantha.
30756330	8	5	theme	species	1290:1296	arg1	composition					1268:1278	the composition	1264:1278	the composition of insect species associated with the plants	1264:1323	We did not find significant differences in the composition of insect species associated with the plants.
30756330	7	6	theme	highest	1132:1138	arg1	rates					1150:1154	highest breakdown rates	1132:1154	four times highest breakdown rates	1121:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	4	7	with	leucantha	803:811	arg1	wood					830:833	low-density wood	818:833	low-density wood	818:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	7	with	leucantha	803:811	arg1	medium-density					774:787	medium-density	774:787	medium-density	774:787	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	7	with	leucantha	803:811	arg1	high-density					736:747	high-density	736:747	high-density	736:747	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	3	8	theme	harder	577:582	arg1	woods					584:588	harder woods	577:588	harder woods	577:588	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	3	8	theme	harder	577:582	arg1	substrate					599:607	less substrate	594:607	less substrate suitable for biofilm production	594:639	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	1	9	theme	structural	197:206	arg1	compounds					208:216	carbon structural compounds	190:216	carbon structural compounds	190:216	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	6	10	theme	hemicellulose	987:999	arg1	concentrations					946:959	higher concentrations	939:959	higher concentrations of lignin, cellulose, and hemicellulose	939:999	Psychotria grandis shows higher concentrations of lignin, cellulose, and hemicellulose, followed by G. lindeniana and M. leucantha.
30756330	7	11	theme	Breakdown	1046:1054	arg1	rates					1056:1060	Breakdown rates	1046:1060	Breakdown rates	1046:1060	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	7	12	theme	plant	1082:1086	arg1	species					1088:1094	plant species	1082:1094	plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value	1082:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	10	13	contain	have	1535:1538	arg2	species					1551:1557	three more species	1540:1557	three more species	1540:1557	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	10	13	contain	have	1535:1538	arg2	groups					1590:1595	two more insect functional groups	1563:1595	two more insect functional groups	1563:1595	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	10	13	contain	have	1535:1538	arg1	Plants					1495:1500	Plants	1495:1500	Plants that decompose faster on average	1495:1533	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	4	14	theme	Gomidesia	710:718	arg1	lindeniana					720:729	Gomidesia lindeniana	710:729	Gomidesia lindeniana with high-density	710:747	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	7	15	theme	richness	1205:1212	arg1	value					1214:1218	average three more species in the species richness value	1163:1218	average three more species in the species richness value	1163:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	1	16	theme	wood	132:135	arg1	densities					144:152	hardness or different wood carbon densities	110:152	densities	144:152	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	7	17	from	species	1182:1188	arg1	species					1197:1203	the species	1193:1203	the species	1193:1203	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	3	18	from	abundant	548:555	arg1	softwood					560:567	softwood	560:567	softwood	560:567	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	0	19	theme	Cuban	59:63	arg1	Stream					84:89	a Cuban Montane Rainforest Stream	57:89	a Cuban Montane Rainforest Stream	57:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	0	20	from	Effects	0:6	arg1	Communities					42:52	Aquatic Insect Communities	27:52	Aquatic Insect Communities in a Cuban Montane Rainforest Stream	27:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	3	21	from	softwood	560:567	arg1	abundant					548:555	abundant	548:555	abundant	548:555	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	0	22	theme	Rainforest	73:82	arg1	Stream					84:89	a Cuban Montane Rainforest Stream	57:89	a Cuban Montane Rainforest Stream	57:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	4	23	theme	low-density	818:828	arg1	wood					830:833	low-density wood	818:833	low-density wood	818:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	2	24	theme	different	443:451	arg1	communities					375:385	aquatic insect communities	360:385	aquatic insect communities with species richness and functional food group profile different from softwoods	360:466	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	9	25	theme	aquatic	1393:1399	arg1	communities					1408:1418	aquatic insect communities	1393:1418	aquatic insect communities	1393:1418	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	2	26	theme	insect	368:373	arg1	communities					375:385	aquatic insect communities	360:385	aquatic insect communities with species richness and functional food group profile different from softwoods	360:466	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	9	27	theme	functional	1366:1375	arg1	organization					1377:1388	functional organization	1366:1388	functional organization	1366:1388	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	2	28	theme	group	429:433	arg1	profile					435:441	functional food group profile	413:441	functional food group profile	413:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	28	theme	group	429:433	arg1	species					392:398	species richness and functional food group profile	392:441	species richness and functional food group profile	392:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	29	theme	functional	413:422	arg1	profile					435:441	functional food group profile	413:441	functional food group profile	413:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	29	theme	functional	413:422	arg1	species					392:398	species richness and functional food group profile	392:441	species richness and functional food group profile	392:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	0	30	theme	Wood	11:14	arg1	Density					16:22	Wood Density	11:22	Wood Density	11:22	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	1	31	theme	aquatic	279:285	arg1	insects					287:293	aquatic insects	279:293	aquatic insects	279:293	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	6	32	theme	Psychotria	914:923	arg1	grandis					925:931	Psychotria grandis	914:931	Psychotria grandis	914:931	Psychotria grandis shows higher concentrations of lignin, cellulose, and hemicellulose, followed by G. lindeniana and M. leucantha.
30756330	7	33	contain	having	1114:1119	arg1	leucantha					1104:1112	M. leucantha	1101:1112	M. leucantha having four times highest breakdown rates	1101:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	7	33	contain	having	1114:1119	arg2	times					1126:1130	four times	1121:1130	four times highest breakdown rates	1121:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	9	34	theme	insect	1401:1406	arg1	communities					1408:1418	aquatic insect communities	1393:1418	aquatic insect communities	1393:1418	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	7	35	theme	species	1182:1188	arg1	value					1214:1218	average three more species in the species richness value	1163:1218	average three more species in the species richness value	1163:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	1	36	theme	tropical	300:307	arg1	stream					309:314	a tropical stream	298:314	a tropical stream	298:314	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	1	37	theme	compounds	208:216	arg1	concentration					173:185	the concentration	169:185	the concentration of carbon structural compounds	169:216	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	4	38	theme	Aquatic	642:648	arg1	insects					650:656	Aquatic insects	642:656	Aquatic insects associated with the following plants	642:693	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	6	39	theme	cellulose	972:980	arg1	concentrations					946:959	higher concentrations	939:959	higher concentrations of lignin, cellulose, and hemicellulose	939:999	Psychotria grandis shows higher concentrations of lignin, cellulose, and hemicellulose, followed by G. lindeniana and M. leucantha.
30756330	10	40	theme	insect	1572:1577	arg1	groups					1590:1595	two more insect functional groups	1563:1595	two more insect functional groups	1563:1595	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	7	41	theme	average	1163:1169	arg1	value					1214:1218	average three more species in the species richness value	1163:1218	average three more species in the species richness value	1163:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	11	42	theme	carbon	1641:1646	arg1	trees					1656:1660	high carbon density trees	1636:1660	high carbon density trees	1636:1660	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	1	43	theme	feeding	240:246	arg1	groups					248:253	functional feeding groups	229:253	functional feeding groups	229:253	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	6	44	theme	lignin	964:969	arg1	concentrations					946:959	higher concentrations	939:959	higher concentrations of lignin, cellulose, and hemicellulose	939:999	Psychotria grandis shows higher concentrations of lignin, cellulose, and hemicellulose, followed by G. lindeniana and M. leucantha.
30756330	11	45	theme	tropical	1665:1672	arg1	forests					1674:1680	tropical forests	1665:1680	tropical forests	1665:1680	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	9	46	theme	lignin	1467:1472	arg1	woods					1488:1492	the woods	1484:1492	the woods	1484:1492	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	9	46	theme	lignin	1467:1472	arg1	amount					1474:1479	lignin amount	1467:1479	lignin amount of the woods	1467:1492	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	4	47	with	lindeniana	720:729	arg1	wood					830:833	low-density wood	818:833	low-density wood	818:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	47	with	lindeniana	720:729	arg1	medium-density					774:787	medium-density	774:787	medium-density	774:787	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	47	with	lindeniana	720:729	arg1	high-density					736:747	high-density	736:747	high-density	736:747	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	7	48	theme	breakdown	1140:1148	arg1	rates					1150:1154	highest breakdown rates	1132:1154	four times highest breakdown rates	1121:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	0	49	theme	Insect	35:40	arg1	Communities					42:52	Aquatic Insect Communities	27:52	Aquatic Insect Communities in a Cuban Montane Rainforest Stream	27:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	8	50	from	differences	1249:1259	arg1	composition					1268:1278	the composition	1264:1278	the composition of insect species associated with the plants	1264:1323	We did not find significant differences in the composition of insect species associated with the plants.
30756330	4	51	theme	following	678:686	arg1	plants					688:693	the following plants	674:693	the following plants	674:693	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	11	52	theme	aquatic	1693:1699	arg1	biodiversity					1701:1712	aquatic biodiversity	1693:1712	aquatic biodiversity	1693:1712	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	1	53	from	richness	267:274	arg1	stream					309:314	a tropical stream	298:314	a tropical stream	298:314	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	9	54	theme	woods	1488:1492	arg1	woods					1488:1492	the woods	1484:1492	the woods	1484:1492	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	9	54	theme	woods	1488:1492	arg1	rates					1457:1461	the breakdown rates	1443:1461	the breakdown rates	1443:1461	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	9	54	theme	woods	1488:1492	arg1	amount					1474:1479	lignin amount	1467:1479	lignin amount of the woods	1467:1492	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	8	55	theme	insect	1283:1288	arg1	species					1290:1296	insect species	1283:1296	insect species associated with the plants	1283:1323	We did not find significant differences in the composition of insect species associated with the plants.
30756330	5	56	theme	abundant	876:883	arg1	Diptera					836:842	Diptera	836:842	Diptera	836:842	Diptera and Ephemeroptera were the most abundant groups sampled in the woods.
30756330	5	56	theme	abundant	876:883	arg1	groups					885:890	the most abundant groups	867:890	the most abundant groups sampled in the woods	867:911	Diptera and Ephemeroptera were the most abundant groups sampled in the woods.
30756330	5	56	theme	abundant	876:883	arg1	Ephemeroptera					848:860	Ephemeroptera	848:860	Ephemeroptera	848:860	Diptera and Ephemeroptera were the most abundant groups sampled in the woods.
30756330	7	57	dep	times	1126:1130	arg1	rates					1150:1154	highest breakdown rates	1132:1154	four times highest breakdown rates	1121:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	3	58	theme	suitable	609:616	arg1	woods					584:588	harder woods	577:588	harder woods	577:588	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	3	58	theme	suitable	609:616	arg1	substrate					599:607	less substrate	594:607	less substrate suitable for biofilm production	594:639	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	1	59	theme	carbon	190:195	arg1	compounds					208:216	carbon structural compounds	190:216	carbon structural compounds	190:216	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	8	60	theme	significant	1237:1247	arg1	differences					1249:1259	significant differences	1237:1259	significant differences in the composition of insect species associated with the plants	1237:1323	We did not find significant differences in the composition of insect species associated with the plants.
30756330	1	61	theme	hardness	110:117	arg1	densities					144:152	hardness or different wood carbon densities	110:152	densities	144:152	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	4	62	theme	Psychotria	750:759	arg1	grandis					761:767	Psychotria grandis	750:767	Psychotria grandis with medium-density	750:787	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	3	63	theme	biofilm	622:628	arg1	production					630:639	biofilm production	622:639	biofilm production	622:639	We also expected that collector-gatherers and collector-filterers will be more abundant in softwood because harder woods are less substrate suitable for biofilm production.
30756330	1	64	theme	different	122:130	arg1	densities					144:152	hardness or different wood carbon densities	110:152	densities	144:152	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	0	65	theme	Montane	65:71	arg1	Stream					84:89	a Cuban Montane Rainforest Stream	57:89	a Cuban Montane Rainforest Stream	57:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	1	66	theme	carbon	137:142	arg1	densities					144:152	hardness or different wood carbon densities	110:152	densities	144:152	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	0	67	from	Communities	42:52	arg1	Stream					84:89	a Cuban Montane Rainforest Stream	57:89	a Cuban Montane Rainforest Stream	57:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	2	68	from	softwoods	458:466	arg1	different					443:451	different	443:451	different	443:451	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	4	69	theme	Meriania	794:801	arg1	leucantha					803:811	Meriania leucantha	794:811	Meriania leucantha with low-density wood	794:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	7	70	with	species	1088:1094	arg1	leucantha					1104:1112	M. leucantha	1101:1112	M. leucantha having four times highest breakdown rates	1101:1154	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	2	71	theme	aquatic	360:366	arg1	communities					375:385	aquatic insect communities	360:385	aquatic insect communities with species richness and functional food group profile different from softwoods	360:466	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	0	72	theme	Density	16:22	arg1	Effects					0:6	Effects	0:6	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.	0:90	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	2	73	theme	food	424:427	arg1	profile					435:441	functional food group profile	413:441	functional food group profile	413:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	73	theme	food	424:427	arg1	species					392:398	species richness and functional food group profile	392:441	species richness and functional food group profile	392:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	0	74	theme	Aquatic	27:33	arg1	Communities					42:52	Aquatic Insect Communities	27:52	Aquatic Insect Communities in a Cuban Montane Rainforest Stream	27:89	Effects of Wood Density on Aquatic Insect Communities in a Cuban Montane Rainforest Stream.
30756330	1	75	theme	insects	287:293	arg1	richness					267:274	functional feeding groups and species richness	229:274	functional feeding groups and species richness of aquatic insects in a tropical stream	229:314	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	11	76	from	loss	1628:1631	arg1	forests					1674:1680	tropical forests	1665:1680	tropical forests	1665:1680	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	9	77	dep	richness	1353:1360	arg1	the					1349:1351	the	1349:1351	the	1349:1351	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	2	78	dep	species	392:398	arg1	profile					435:441	functional food group profile	413:441	functional food group profile	413:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	78	dep	species	392:398	arg1	richness					400:407	richness	400:407	richness	400:407	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	2	78	dep	species	392:398	arg1	species					392:398	species richness and functional food group profile	392:441	species richness and functional food group profile	392:441	We expected that harder woods would harbor aquatic insect communities with species richness and functional food group profile different from softwoods.
30756330	7	79	theme	more	1177:1180	arg1	species					1182:1188	three more species	1171:1188	average three more species in the species richness value	1163:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	9	80	theme	communities	1408:1418	arg1	richness					1353:1360	richness	1353:1360	richness	1353:1360	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	9	80	theme	communities	1408:1418	arg1	organization					1377:1388	functional organization	1366:1388	functional organization	1366:1388	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30756330	11	81	theme	trees	1656:1660	arg1	loss					1628:1631	the loss	1624:1631	the loss of high carbon density trees in tropical forests	1624:1680	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	4	82	dep	analyzed	700:707	arg1	lindeniana					720:729	Gomidesia lindeniana	710:729	Gomidesia lindeniana with high-density	710:747	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	82	dep	analyzed	700:707	arg1	leucantha					803:811	Meriania leucantha	794:811	Meriania leucantha with low-density wood	794:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	82	dep	analyzed	700:707	arg1	grandis					761:767	Psychotria grandis	750:767	Psychotria grandis with medium-density	750:787	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	10	83	theme	more	1546:1549	arg1	species					1551:1557	three more species	1540:1557	three more species	1540:1557	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	11	84	theme	density	1648:1654	arg1	trees					1656:1660	high carbon density trees	1636:1660	high carbon density trees	1636:1660	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	7	85	from	species	1088:1094	arg1	value					1214:1218	average three more species in the species richness value	1163:1218	average three more species in the species richness value	1163:1218	Breakdown rates are different among plant species with M. leucantha having four times highest breakdown rates and on average three more species in the species richness value.
30756330	10	86	theme	functional	1579:1588	arg1	groups					1590:1595	two more insect functional groups	1563:1595	two more insect functional groups	1563:1595	Plants that decompose faster on average have three more species and two more insect functional groups.
30756330	4	87	with	grandis	761:767	arg1	wood					830:833	low-density wood	818:833	low-density wood	818:833	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	87	with	grandis	761:767	arg1	medium-density					774:787	medium-density	774:787	medium-density	774:787	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	4	87	with	grandis	761:767	arg1	high-density					736:747	high-density	736:747	high-density	736:747	Aquatic insects associated with the following plants were analyzed: Gomidesia lindeniana with high-density, Psychotria grandis with medium-density, and Meriania leucantha with low-density wood.
30756330	1	88	theme	functional	229:238	arg1	groups					248:253	functional feeding groups	229:253	functional feeding groups	229:253	We tested whether hardness or different wood carbon densities, considered as the concentration of carbon structural compounds, influence functional feeding groups and species richness of aquatic insects in a tropical stream.
30756330	11	89	theme	high	1636:1639	arg1	trees					1656:1660	high carbon density trees	1636:1660	high carbon density trees	1636:1660	Our findings suggest that the loss of high carbon density trees in tropical forests can affect aquatic biodiversity.
30756330	9	90	theme	breakdown	1447:1455	arg1	rates					1457:1461	the breakdown rates	1443:1461	the breakdown rates	1443:1461	We found evidence that the richness and functional organization of aquatic insect communities were mostly related to the breakdown rates and lignin amount of the woods.
30682479	2	0	theme	gel	437:439	arg1	firmness					415:422	the firmness	411:422	the firmness of egg white gel	411:439	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	1	1	dep	values	288:293	arg1	4.0					296:298	4.0	296:298	4.0	296:298	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	1	1	dep	values	288:293	arg1	7.0					304:306	7.0	304:306	7.0	304:306	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	4	2	theme	composite	806:814	arg1	hydrogels					833:841	the composite gellan/egg white hydrogels	802:841	the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0	802:866	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	5	3	theme	white	1076:1080	arg1	proteins					1082:1089	egg white proteins	1072:1089	egg white proteins	1072:1089	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	1	4	theme	egg	239:241	arg1	hydrogels					262:270	egg white heat-induced hydrogels	239:270	egg white heat-induced hydrogels	239:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	0	5	theme	functional	56:65	arg1	properties					84:93	the structural, functional, and degradation properties	40:93	the structural, functional, and degradation properties of egg white heat-induced hydrogels	40:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	5	6	theme	gellan	1095:1100	arg1	gum					1102:1104	gellan gum	1095:1104	gellan gum	1095:1104	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	4	7	theme	gellan/egg	816:825	arg1	hydrogels					833:841	the composite gellan/egg white hydrogels	802:841	the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0	802:866	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	1	8	theme	white	243:247	arg1	hydrogels					262:270	egg white heat-induced hydrogels	239:270	egg white heat-induced hydrogels	239:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	0	9	theme	degradation	72:82	arg1	properties					84:93	the structural, functional, and degradation properties	40:93	the structural, functional, and degradation properties of egg white heat-induced hydrogels	40:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	0	10	from	Effects	0:6	arg1	properties					84:93	the structural, functional, and degradation properties	40:93	the structural, functional, and degradation properties of egg white heat-induced hydrogels	40:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	4	11	theme	white	827:831	arg1	hydrogels					833:841	the composite gellan/egg white hydrogels	802:841	the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0	802:866	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	2	12	theme	hydrogels	586:594	arg1	rupturing					559:567	the rupturing	555:567	the rupturing of the egg white hydrogels	555:594	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	6	13	with	enrichment	1150:1159	arg1	gum					1191:1193	gellan gum	1184:1193	gellan gum	1184:1193	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	1	14	theme	heat-induced	249:260	arg1	hydrogels					262:270	egg white heat-induced hydrogels	239:270	egg white heat-induced hydrogels	239:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	3	15	theme	egg	664:666	arg1	hydrogels					674:682	egg white hydrogels	664:682	egg white hydrogels	664:682	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	2	16	theme	compressive	523:533	arg1	stress					535:540	the compressive stress	519:540	the compressive stress required for the rupturing of the egg white hydrogels	519:594	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	2	17	theme	white	580:584	arg1	hydrogels					586:594	the egg white hydrogels	572:594	the egg white hydrogels	572:594	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	1	18	theme	hydrogels	262:270	arg1	properties					225:234	the technological functionality and in vitro degradation properties	168:234	the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels	168:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	4	19	theme	corresponding	904:916	arg1	hydrogels					953:961	the corresponding single-component egg white protein hydrogels	900:961	the corresponding single-component egg white protein hydrogels	900:961	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	3	20	theme	white	668:672	arg1	hydrogels					674:682	egg white hydrogels	664:682	egg white hydrogels	664:682	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	2	21	theme	egg	576:578	arg1	hydrogels					586:594	the egg white hydrogels	572:594	the egg white hydrogels	572:594	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	6	22	theme	gastrointestinal	1250:1265	arg1	degradation					1267:1277	in vitro gastrointestinal degradation	1241:1277	in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery	1241:1354	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	3	23	with	enrichment	601:610	arg1	gellan					617:622	gellan	617:622	gellan	617:622	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	6	24	theme	egg	1164:1166	arg1	gels					1174:1177	egg white gels	1164:1177	egg white gels	1164:1177	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	6	25	theme	site-specific	1323:1335	arg1	delivery					1347:1354	the site-specific bioactive delivery	1319:1354	the site-specific bioactive delivery	1319:1354	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	5	26	theme	egg	1072:1074	arg1	proteins					1082:1089	egg white proteins	1072:1089	egg white proteins	1072:1089	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	4	27	theme	denser	872:877	arg1	microstructures					879:893	denser microstructures	872:893	denser microstructures	872:893	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	6	28	theme	bioactive	1337:1345	arg1	delivery					1347:1354	the site-specific bioactive delivery	1319:1354	the site-specific bioactive delivery	1319:1354	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	2	29	theme	white	431:435	arg1	gel					437:439	egg white gel	427:439	egg white gel	427:439	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	6	30	theme	gellan	1184:1189	arg1	gum					1191:1193	gellan gum	1184:1193	gellan gum	1184:1193	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	5	31	dep	Fourier	968:974	arg1	transform					976:984	transform	976:984	transform infra-red (FT-IR) spectroscopy	976:1015	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	2	32	theme	gellan	354:359	arg1	gum					361:363	gellan gum	354:363	gellan gum	354:363	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	2	33	theme	gellan	472:477	arg1	addition					460:467	the addition	456:467	the addition of gellan	456:477	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	6	34	dep	in	1241:1242	arg1	vitro					1244:1248	vitro	1244:1248	vitro	1244:1248	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	4	35	contain	had	868:870	arg2	microstructures					879:893	denser microstructures	872:893	denser microstructures	872:893	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	4	35	contain	had	868:870	arg1	hydrogels					833:841	the composite gellan/egg white hydrogels	802:841	the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0	802:866	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	1	36	theme	different	275:283	arg1	values					288:293	different pH values	275:293	different pH values (4.0 and 7.0)	275:307	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	0	37	theme	white	102:106	arg1	hydrogels					121:129	egg white heat-induced hydrogels	98:129	egg white heat-induced hydrogels	98:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	6	38	theme	gels	1174:1177	arg1	enrichment					1150:1159	the enrichment	1146:1159	the enrichment of egg white gels with gellan gum	1146:1193	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	1	39	theme	technological	172:184	arg1	functionality					186:198	technological functionality	172:198	technological functionality	172:198	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	1	40	theme	pH	285:286	arg1	values					288:293	different pH values	275:293	different pH values (4.0 and 7.0)	275:307	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	0	41	theme	egg	98:100	arg1	hydrogels					121:129	egg white heat-induced hydrogels	98:129	egg white heat-induced hydrogels	98:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	1	42	theme	functionality	186:198	arg1	properties					225:234	the technological functionality and in vitro degradation properties	168:234	the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels	168:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	2	43	theme	egg	427:429	arg1	gel					437:439	egg white gel	427:439	egg white gel	427:439	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	0	44	theme	hydrogels	121:129	arg1	properties					84:93	the structural, functional, and degradation properties	40:93	the structural, functional, and degradation properties of egg white heat-induced hydrogels	40:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	4	45	theme	electron	761:768	arg1	SEM					782:784	SEM	782:784	SEM	782:784	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	4	45	theme	electron	761:768	arg1	microscopy					770:779	The scanning electron microscopy	748:779	The scanning electron microscopy (SEM)	748:785	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	0	46	theme	gellan	26:31	arg1	gum					33:35	gellan gum	26:35	gellan gum	26:35	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	4	47	theme	white	939:943	arg1	hydrogels					953:961	the corresponding single-component egg white protein hydrogels	900:961	the corresponding single-component egg white protein hydrogels	900:961	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	0	48	theme	heat-induced	108:119	arg1	hydrogels					121:129	egg white heat-induced hydrogels	98:129	egg white heat-induced hydrogels	98:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	4	49	theme	scanning	752:759	arg1	SEM					782:784	SEM	782:784	SEM	782:784	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	4	49	theme	scanning	752:759	arg1	microscopy					770:779	The scanning electron microscopy	748:779	The scanning electron microscopy (SEM)	748:785	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	1	50	theme	in	204:205	arg1	degradation					213:223	in vitro degradation	204:223	in vitro degradation	204:223	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	3	51	theme	water	724:728	arg1	capacity					738:745	their water holding capacity	718:745	their water holding capacity	718:745	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	4	52	theme	single-component	918:933	arg1	hydrogels					953:961	the corresponding single-component egg white protein hydrogels	900:961	the corresponding single-component egg white protein hydrogels	900:961	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	5	53	theme	FT-IR	997:1001	arg1	spectroscopy					1004:1015	infra-red (FT-IR) spectroscopy	986:1015	infra-red (FT-IR) spectroscopy	986:1015	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	6	54	theme	in	1241:1242	arg1	degradation					1267:1277	in vitro gastrointestinal degradation	1241:1277	in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery	1241:1354	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	5	55	used	used	1026:1029	arg2	Fourier					968:974	The Fourier	964:974	The Fourier transform infra-red (FT-IR) spectroscopy	964:1015	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	6	56	theme	white	1168:1172	arg1	gels					1174:1177	egg white gels	1164:1177	egg white gels	1164:1177	Furthermore, the results revealed that the enrichment of egg white gels with gellan gum drastically increased their stability against in vitro gastrointestinal degradation which can enhance their applications in the site-specific bioactive delivery.
30682479	5	57	theme	infra-red	986:994	arg1	spectroscopy					1004:1015	infra-red (FT-IR) spectroscopy	986:1015	infra-red (FT-IR) spectroscopy	986:1015	The Fourier transform infra-red (FT-IR) spectroscopy also was used to characterize the interactions between egg white proteins and gellan gum.
30682479	3	58	theme	hydrogels	674:682	arg1	behavior					652:659	the swelling behavior	639:659	the swelling behavior of egg white hydrogels	639:682	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	2	59	with	enrichment	338:347	arg1	gum					361:363	gellan gum	354:363	gellan gum	354:363	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	1	60	dep	in	204:205	arg1	vitro					207:211	vitro	207:211	vitro	207:211	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	2	61	from	value	371:375	arg1	enrichment					338:347	the enrichment	334:347	the enrichment with gellan gum at pH value of 7.0	334:382	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	1	62	theme	degradation	213:223	arg1	properties					225:234	the technological functionality and in vitro degradation properties	168:234	the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels	168:270	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30682479	4	63	theme	egg	935:937	arg1	hydrogels					953:961	the corresponding single-component egg white protein hydrogels	900:961	the corresponding single-component egg white protein hydrogels	900:961	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	2	64	theme	pH	368:369	arg1	value					371:375	pH value	368:375	pH value of 7.0	368:382	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	2	65	theme	7.0	380:382	arg1	value					371:375	pH value	368:375	pH value of 7.0	368:382	The results showed that the enrichment with gellan gum at pH value of 7.0 did not drastically modify the firmness of egg white gel, but at pH 4.0 the addition of gellan significantly improved the hardness and the compressive stress required for the rupturing of the egg white hydrogels.
30682479	3	66	theme	holding	730:736	arg1	capacity					738:745	their water holding capacity	718:745	their water holding capacity	718:745	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	0	67	theme	structural	44:53	arg1	properties					84:93	the structural, functional, and degradation properties	40:93	the structural, functional, and degradation properties of egg white heat-induced hydrogels	40:129	Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels.
30682479	4	68	theme	protein	945:951	arg1	hydrogels					953:961	the corresponding single-component egg white protein hydrogels	900:961	the corresponding single-component egg white protein hydrogels	900:961	The scanning electron microscopy (SEM) indicated that the composite gellan/egg white hydrogels formed at pH 4.0 and 7.0 had denser microstructures than the corresponding single-component egg white protein hydrogels.
30682479	3	69	theme	swelling	643:650	arg1	behavior					652:659	the swelling behavior	639:659	the swelling behavior of egg white hydrogels	639:682	The enrichment with gellan also modulated the swelling behavior of egg white hydrogels as well as significantly improved their water holding capacity.
30682479	1	70	theme	Gellan	132:137	arg1	gum					139:141	Gellan gum	132:141	Gellan gum	132:141	Gellan gum was employed to modulate the technological functionality and in vitro degradation properties of egg white heat-induced hydrogels at different pH values (4.0 and 7.0).
30806252	6	0	theme	spleen	1203:1208	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	5	1	theme	CD4+	985:988	arg1	lymphocytes					1000:1010	CD4+ to CD8+ T lymphocytes	985:1010	CD4+ to CD8+ T lymphocytes	985:1010	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	2	from	indexes	791:797	arg1	thymus					773:778	the thymus	769:778	the thymus	769:778	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	3	theme	lymphocytes	956:966	arg1	counts					937:942	the counts	933:942	the counts of CD3+CD4+ lymphocytes	933:966	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	3	theme	lymphocytes	956:966	arg1	ratio					976:980	the ratio	972:980	the ratio of CD4+ to CD8+ T lymphocytes	972:1010	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	4	from	Activities	17:26	arg1	bisporus					84:91	bisporus	84:91	bisporus	84:91	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	4	from	Activities	17:26	arg1	Bodies					120:125	Fruiting Bodies	111:125	Fruiting Bodies	111:125	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	4	from	Activities	17:26	arg1	Mycelia					140:146	Cultured Mycelia	131:146	Cultured Mycelia	131:146	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	4	from	Activities	17:26	arg1	Mushroom					65:72	White Button Mushroom	52:72	White Button Mushroom	52:72	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	7	5	theme	immunotherapeutic	1471:1487	arg1	FPS					1389:1391	FPS	1389:1391	FPS	1389:1391	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	5	theme	immunotherapeutic	1471:1487	arg1	IPS					1394:1396	IPS	1394:1396	IPS	1394:1396	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	5	theme	immunotherapeutic	1471:1487	arg1	EPS					1403:1405	EPS	1403:1405	EPS	1403:1405	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	5	theme	immunotherapeutic	1471:1487	arg1	medicines					1489:1497	potential immunotherapeutic medicines	1461:1497	potential immunotherapeutic medicines	1461:1497	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	5	6	theme	serum	818:822	arg1	cytokines					824:832	serum cytokines	818:832	serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)	818:927	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	7	theme	thymus	1192:1197	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	5	8	theme	CD8+	993:996	arg1	lymphocytes					1000:1010	CD4+ to CD8+ T lymphocytes	985:1010	CD4+ to CD8+ T lymphocytes	985:1010	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	2	9	from	methylation	333:343	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	9	from	methylation	333:343	arg1	mycelia					446:452	cultured mycelia	437:452	cultured mycelia (IPS)	437:458	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	9	from	methylation	333:343	arg1	broth					478:482	fermentation broth	465:482	fermentation broth (EPS)	465:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	9	from	methylation	333:343	arg1	IPS					455:457	IPS	455:457	IPS	455:457	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	9	from	methylation	333:343	arg1	EPS					485:487	EPS	485:487	EPS	485:487	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	9	from	methylation	333:343	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	mycelia					446:452	cultured mycelia	437:452	cultured mycelia (IPS)	437:458	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	broth					478:482	fermentation broth	465:482	fermentation broth (EPS)	465:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	IPS					455:457	IPS	455:457	IPS	455:457	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	EPS					485:487	EPS	485:487	EPS	485:487	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	10	from	composition	320:330	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	5	11	theme	CD3+CD8+	1051:1058	arg1	lymphocytes					1060:1070	CD3+CD8+ lymphocytes	1051:1070	CD3+CD8+ lymphocytes	1051:1070	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	12	from	Mushroom	65:72	arg1	Polysaccharides					31:45	Polysaccharides	31:45	Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice	31:183	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	12	from	Mushroom	65:72	arg1	Activities					17:26	Immunomodulatory Activities	0:26	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.	0:184	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	6	13	theme	immunoglobin	1345:1356	arg1	G					1358:1358	immunoglobin G	1345:1358	immunoglobin G	1345:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	5	14	theme	lymphocytes	1060:1070	arg1	counts					1041:1046	the counts	1037:1046	the counts of CD3+CD8+ lymphocytes in normal mice	1037:1085	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	15	theme	Fruiting	111:118	arg1	Bodies					120:125	Fruiting Bodies	111:125	Fruiting Bodies	111:125	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	15	theme	Fruiting	111:118	arg1	Mushroom					65:72	White Button Mushroom	52:72	White Button Mushroom	52:72	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	5	16	theme	cytokines	824:832	arg1	amounts					807:813	the amounts	803:813	the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)	803:927	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	16	theme	cytokines	824:832	arg1	cytokines					824:832	serum cytokines	818:832	serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)	818:927	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	17	theme	cyclophosphamide-treated	1100:1123	arg1	mice					1125:1128	cyclophosphamide-treated mice	1100:1128	cyclophosphamide-treated mice	1100:1128	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	7	18	theme	immune	1528:1533	arg1	function					1535:1542	inadequate immune function	1517:1542	inadequate immune function	1517:1542	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	5	19	theme	tumor	871:875	arg1	TNF-α					896:900	TNF-α	896:900	TNF-α	896:900	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	19	theme	tumor	871:875	arg1	factor-α					886:893	tumor necrosis factor-α	871:893	tumor necrosis factor-α (TNF-α)	871:901	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	20	theme	spleen	784:789	arg1	indexes					791:797	spleen indexes	784:797	spleen indexes	784:797	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	21	theme	lymphocyte	1219:1228	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	22	theme	fermentation	465:476	arg1	EPS					485:487	EPS	485:487	EPS	485:487	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	22	theme	fermentation	465:476	arg1	broth					478:482	fermentation broth	465:482	fermentation broth (EPS)	465:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	5	23	theme	necrosis	877:884	arg1	TNF-α					896:900	TNF-α	896:900	TNF-α	896:900	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	23	theme	necrosis	877:884	arg1	factor-α					886:893	tumor necrosis factor-α	871:893	tumor necrosis factor-α (TNF-α)	871:901	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	24	theme	phagocytotic	1245:1256	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	25	theme	fruiting	414:421	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	25	theme	fruiting	414:421	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	6	26	theme	macrophages	1281:1291	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	26	theme	macrophages	1281:1291	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	26	theme	macrophages	1281:1291	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	26	theme	macrophages	1281:1291	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	5	27	dep	lymphocytes	1000:1010	arg1	to					990:991	to	990:991	to	990:991	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	2	28	from	activities	367:376	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	28	from	activities	367:376	arg1	mycelia					446:452	cultured mycelia	437:452	cultured mycelia (IPS)	437:458	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	28	from	activities	367:376	arg1	broth					478:482	fermentation broth	465:482	fermentation broth (EPS)	465:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	28	from	activities	367:376	arg1	IPS					455:457	IPS	455:457	IPS	455:457	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	28	from	activities	367:376	arg1	EPS					485:487	EPS	485:487	EPS	485:487	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	28	from	activities	367:376	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	4	29	theme	different	670:678	arg1	structures					695:704	different polysaccharide structures	670:704	different polysaccharide structures	670:704	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	5	30	from	increases	756:764	arg1	thymus					773:778	the thymus	769:778	the thymus	769:778	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	2	31	theme	bisporus	405:412	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	31	theme	bisporus	405:412	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	0	32	theme	Immunomodulatory	0:15	arg1	Activities					17:26	Immunomodulatory Activities	0:26	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.	0:184	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	6	33	from	mice	1125:1128	arg1	able					1158:1161	able	1158:1161	able	1158:1161	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	33	from	mice	1125:1128	arg1	FPS					1135:1137	the FPS	1131:1137	the FPS	1131:1137	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	34	theme	A.	402:403	arg1	FPS					431:433	FPS	431:433	FPS	431:433	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	34	theme	A.	402:403	arg1	bodies					423:428	A. bisporus fruiting bodies	402:428	A. bisporus fruiting bodies (FPS)	402:434	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	0	35	theme	Polysaccharides	31:45	arg1	Activities					17:26	Immunomodulatory Activities	0:26	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.	0:184	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	5	36	theme	CD3+CD4+	947:954	arg1	lymphocytes					956:966	CD3+CD4+ lymphocytes	947:966	CD3+CD4+ lymphocytes	947:966	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	37	theme	TNF-α	1334:1338	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	37	theme	TNF-α	1334:1338	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	37	theme	TNF-α	1334:1338	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	37	theme	TNF-α	1334:1338	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	38	theme	monosaccharide	305:318	arg1	composition					320:330	the monosaccharide composition	301:330	the monosaccharide composition	301:330	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	6	39	theme	IL-17	1327:1331	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	39	theme	IL-17	1327:1331	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	39	theme	IL-17	1327:1331	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	39	theme	IL-17	1327:1331	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	40	theme	peritoneal	1270:1279	arg1	macrophages					1281:1291	peritoneal macrophages	1270:1291	peritoneal macrophages	1270:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	41	theme	cultured	437:444	arg1	IPS					455:457	IPS	455:457	IPS	455:457	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	41	theme	cultured	437:444	arg1	mycelia					446:452	cultured mycelia	437:452	cultured mycelia (IPS)	437:458	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	4	42	contain	possessed	660:668	arg1	FPS					642:644	FPS	642:644	FPS	642:644	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	4	42	contain	possessed	660:668	arg2	structures					695:704	different polysaccharide structures	670:704	different polysaccharide structures	670:704	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	4	42	contain	possessed	660:668	arg1	IPS					647:649	IPS	647:649	IPS	647:649	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	4	42	contain	possessed	660:668	arg1	EPS					656:658	EPS	656:658	EPS	656:658	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	4	43	theme	polysaccharide	680:693	arg1	structures					695:704	different polysaccharide structures	670:704	different polysaccharide structures	670:704	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	5	44	theme	remarkable	745:754	arg1	increases					756:764	remarkable increases	745:764	remarkable increases in the thymus	745:778	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	45	theme	White	52:56	arg1	bisporus					84:91	bisporus	84:91	bisporus	84:91	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	45	theme	White	52:56	arg1	Bodies					120:125	Fruiting Bodies	111:125	Fruiting Bodies	111:125	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	45	theme	White	52:56	arg1	Mycelia					140:146	Cultured Mycelia	131:146	Cultured Mycelia	131:146	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	45	theme	White	52:56	arg1	Mushroom					65:72	White Button Mushroom	52:72	White Button Mushroom	52:72	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	6	46	from	able	1158:1161	arg1	mice					1125:1128	cyclophosphamide-treated mice	1100:1128	cyclophosphamide-treated mice	1100:1128	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	5	47	from	counts	1041:1046	arg1	mice					1082:1085	normal mice	1075:1085	normal mice	1075:1085	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	2	48	from	broth	478:482	arg1	polysaccharides					381:395	polysaccharides	381:395	polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS)	381:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	48	from	broth	478:482	arg1	methylation					333:343	methylation	333:343	methylation	333:343	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	48	from	broth	478:482	arg1	composition					320:330	the monosaccharide composition	301:330	the monosaccharide composition	301:330	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	48	from	broth	478:482	arg1	activities					367:376	immunomodulatory activities	350:376	immunomodulatory activities	350:376	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	4	49	theme	methylation	607:617	arg1	results					619:625	the methylation results	603:625	the methylation results	603:625	However, the methylation results indicated that FPS, IPS, and EPS possessed different polysaccharide structures.
30806252	5	50	theme	T	998:998	arg1	lymphocytes					1000:1010	CD4+ to CD8+ T lymphocytes	985:1010	CD4+ to CD8+ T lymphocytes	985:1010	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	51	theme	Cultured	131:138	arg1	Mycelia					140:146	Cultured Mycelia	131:146	Cultured Mycelia	131:146	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	51	theme	Cultured	131:138	arg1	Mushroom					65:72	White Button Mushroom	52:72	White Button Mushroom	52:72	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	3	52	theme	glucose	562:568	arg1	EPS					575:577	EPS	575:577	EPS	575:577	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	3	52	theme	glucose	562:568	arg1	IPS					554:556	IPS	554:556	IPS	554:556	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	3	52	theme	glucose	562:568	arg1	mannose					545:551	mannose	545:551	mannose	545:551	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	1	53	theme	important	214:222	arg1	edible					224:229	a very important edible	207:229	a very important edible	207:229	Agaricus bisporus is a very important edible and medicinal mushroom.
30806252	1	53	theme	important	214:222	arg1	bisporus					195:202	bisporus	195:202	bisporus	195:202	Agaricus bisporus is a very important edible and medicinal mushroom.
30806252	5	54	theme	lymphocytes	1000:1010	arg1	counts					937:942	the counts	933:942	the counts of CD3+CD4+ lymphocytes	933:966	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	5	54	theme	lymphocytes	1000:1010	arg1	ratio					976:980	the ratio	972:980	the ratio of CD4+ to CD8+ T lymphocytes	972:1010	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	0	55	theme	Healthy	151:157	arg1	Mice					180:183	Healthy and Immunosuppressed Mice	151:183	Healthy and Immunosuppressed Mice	151:183	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	3	56	theme	galactose	583:591	arg1	EPS					575:577	EPS	575:577	EPS	575:577	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	3	56	theme	galactose	583:591	arg1	IPS					554:556	IPS	554:556	IPS	554:556	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	3	56	theme	galactose	583:591	arg1	mannose					545:551	mannose	545:551	mannose	545:551	The results indicated that FPS was mainly composed of mannose; IPS, of glucose; and EPS, of galactose.
30806252	6	57	theme	IL-10	1320:1324	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	57	theme	IL-10	1320:1324	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	57	theme	IL-10	1320:1324	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	57	theme	IL-10	1320:1324	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	2	58	theme	immunomodulatory	350:365	arg1	activities					367:376	immunomodulatory activities	350:376	immunomodulatory activities	350:376	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	0	59	theme	Button	58:63	arg1	bisporus					84:91	bisporus	84:91	bisporus	84:91	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	59	theme	Button	58:63	arg1	Bodies					120:125	Fruiting Bodies	111:125	Fruiting Bodies	111:125	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	59	theme	Button	58:63	arg1	Mycelia					140:146	Cultured Mycelia	131:146	Cultured Mycelia	131:146	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	0	59	theme	Button	58:63	arg1	Mushroom					65:72	White Button Mushroom	52:72	White Button Mushroom	52:72	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	7	60	with	patients	1503:1510	arg1	function					1535:1542	inadequate immune function	1517:1542	inadequate immune function	1517:1542	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	2	61	from	bodies	423:428	arg1	polysaccharides					381:395	polysaccharides	381:395	polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS)	381:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	61	from	bodies	423:428	arg1	methylation					333:343	methylation	333:343	methylation	333:343	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	61	from	bodies	423:428	arg1	composition					320:330	the monosaccharide composition	301:330	the monosaccharide composition	301:330	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	61	from	bodies	423:428	arg1	activities					367:376	immunomodulatory activities	350:376	immunomodulatory activities	350:376	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	0	62	theme	Immunosuppressed	163:178	arg1	Mice					180:183	Healthy and Immunosuppressed Mice	151:183	Healthy and Immunosuppressed Mice	151:183	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	6	63	theme	IL-6	1314:1317	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	63	theme	IL-6	1314:1317	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	63	theme	IL-6	1314:1317	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	63	theme	IL-6	1314:1317	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	0	64	from	Mycelia	140:146	arg1	Mice					180:183	Healthy and Immunosuppressed Mice	151:183	Healthy and Immunosuppressed Mice	151:183	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
30806252	7	65	theme	potential	1461:1469	arg1	FPS					1389:1391	FPS	1389:1391	FPS	1389:1391	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	65	theme	potential	1461:1469	arg1	IPS					1394:1396	IPS	1394:1396	IPS	1394:1396	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	65	theme	potential	1461:1469	arg1	EPS					1403:1405	EPS	1403:1405	EPS	1403:1405	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	65	theme	potential	1461:1469	arg1	medicines					1489:1497	potential immunotherapeutic medicines	1461:1497	potential immunotherapeutic medicines	1461:1497	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	6	66	theme	G	1358:1358	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	66	theme	G	1358:1358	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	66	theme	G	1358:1358	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	66	theme	G	1358:1358	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	7	67	theme	immunomodulatory	1433:1448	arg1	agents					1450:1455	immunomodulatory agents	1433:1455	immunomodulatory agents	1433:1455	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	67	theme	immunomodulatory	1433:1448	arg1	IPS					1394:1396	IPS	1394:1396	IPS	1394:1396	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	67	theme	immunomodulatory	1433:1448	arg1	EPS					1403:1405	EPS	1403:1405	EPS	1403:1405	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	7	67	theme	immunomodulatory	1433:1448	arg1	FPS					1389:1391	FPS	1389:1391	FPS	1389:1391	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	5	68	theme	normal	1075:1080	arg1	mice					1082:1085	normal mice	1075:1085	normal mice	1075:1085	Furthermore, FPS, IPS, and EPS caused remarkable increases in the thymus and spleen indexes; in the amounts of serum cytokines containing interleukin (IL)-2, IL-4, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); in the counts of CD3+CD4+ lymphocytes and the ratio of CD4+ to CD8+ T lymphocytes; however, they decreased the counts of CD3+CD8+ lymphocytes in normal mice.
30806252	6	69	theme	IL-2	1308:1311	arg1	levels					1298:1303	levels	1298:1303	levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G	1298:1358	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	69	theme	IL-2	1308:1311	arg1	indexes					1210:1216	the thymus and spleen indexes	1188:1216	indexes	1210:1216	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	69	theme	IL-2	1308:1311	arg1	proliferation					1230:1242	lymphocyte proliferation	1219:1242	lymphocyte proliferation	1219:1242	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	6	69	theme	IL-2	1308:1311	arg1	activity					1258:1265	phagocytotic activity	1245:1265	phagocytotic activity of peritoneal macrophages	1245:1291	Finally, in cyclophosphamide-treated mice, the FPS, IPS, and EPS were able to significantly restore the thymus and spleen indexes, lymphocyte proliferation, phagocytotic activity of peritoneal macrophages, and levels of IL-2, IL-6, IL-10, IL-17, TNF-α, and immunoglobin G.
30806252	1	70	theme	medicinal	235:243	arg1	mushroom					245:252	medicinal mushroom	235:252	medicinal mushroom	235:252	Agaricus bisporus is a very important edible and medicinal mushroom.
30806252	2	71	from	mycelia	446:452	arg1	polysaccharides					381:395	polysaccharides	381:395	polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS)	381:488	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	71	from	mycelia	446:452	arg1	methylation					333:343	methylation	333:343	methylation	333:343	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	71	from	mycelia	446:452	arg1	composition					320:330	the monosaccharide composition	301:330	the monosaccharide composition	301:330	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	71	from	mycelia	446:452	arg1	activities					367:376	immunomodulatory activities	350:376	immunomodulatory activities	350:376	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	72	theme	polysaccharides	381:395	arg1	methylation					333:343	methylation	333:343	methylation	333:343	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	72	theme	polysaccharides	381:395	arg1	composition					320:330	the monosaccharide composition	301:330	the monosaccharide composition	301:330	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	2	72	theme	polysaccharides	381:395	arg1	activities					367:376	immunomodulatory activities	350:376	immunomodulatory activities	350:376	In this study, we systematically investigated the monosaccharide composition, methylation, and immunomodulatory activities of polysaccharides from A. bisporus fruiting bodies (FPS), cultured mycelia (IPS), and fermentation broth (EPS).
30806252	7	73	theme	inadequate	1517:1526	arg1	function					1535:1542	inadequate immune function	1517:1542	inadequate immune function	1517:1542	These findings suggest that FPS, IPS, and EPS could all be exploited as immunomodulatory agents and potential immunotherapeutic medicines for patients with inadequate immune function.
30806252	0	74	from	Bodies	120:125	arg1	Mice					180:183	Healthy and Immunosuppressed Mice	151:183	Healthy and Immunosuppressed Mice	151:183	Immunomodulatory Activities of Polysaccharides from White Button Mushroom, Agaricus bisporus (Agaricomycetes), Fruiting Bodies and Cultured Mycelia in Healthy and Immunosuppressed Mice.
31499110	0	0	theme	reproductive	193:204	arg1	performance					206:216	reproductive performance	193:216	reproductive performance	193:216	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	4	1	theme	liquid	671:676	arg1	chromatography					678:691	high-performance liquid chromatography	654:691	high-performance liquid chromatography	654:691	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	9	2	theme	reproductive	1625:1636	arg1	performances					1638:1649	reproductive performances	1625:1649	reproductive performances	1625:1649	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	6	3	theme	X-ray	979:983	arg1	diffraction					985:995	X-ray diffraction	979:995	X-ray diffraction	979:995	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	7	4	theme	hydrogen	1155:1162	arg1	peroxide					1164:1171	hydrogen peroxide	1155:1171	hydrogen peroxide scavenging (66.3 ± 1.16%)	1155:1197	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	8	5	theme	pathogenic	1407:1416	arg1	bacteria					1418:1425	the pathogenic bacteria	1403:1425	the pathogenic bacteria	1403:1425	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	3	6	theme	carbon	501:506	arg1	presence					489:496	the presence	485:496	the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%)	485:575	The elemental analysis confirmed the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%).
31499110	10	7	theme	nutritional	1749:1759	arg1	properties					1761:1770	pharmaceutical as well as nutritional properties	1723:1770	pharmaceutical as well as nutritional properties	1723:1770	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	7	8	theme	total	1268:1272	arg1	activities					1311:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	9	theme	antioxidant	1068:1078	arg1	activity					1080:1087	potent antioxidant activity	1061:1087	potent antioxidant activity in DPPH (76.45 ± 0.49%)	1061:1111	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	10	theme	antioxidant	1274:1284	arg1	activities					1311:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	0	11	dep	In	123:124	arg1	vitro					126:130	vitro	126:130	vitro	126:130	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	10	12	theme	isolated	1684:1691	arg1	SPs					1693:1695	the isolated SPs	1680:1695	the isolated SPs from S. platensis	1680:1713	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	0	13	theme	bioactive	102:110	arg1	potential					112:120	its bioactive potential	98:120	its bioactive potential	98:120	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	8	14	theme	diffusion	1453:1461	arg1	assays					1498:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	3	15	theme	hydrogen	525:532	arg1	presence					489:496	the presence	485:496	the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%)	485:575	The elemental analysis confirmed the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%).
31499110	6	16	theme	UV-visible	954:963	arg1	spectroscopy					965:976	UV-visible spectroscopy	954:976	UV-visible spectroscopy	954:976	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	0	17	theme	In	123:124	arg1	antioxidant					132:142	In vitro antioxidant	123:142	In vitro antioxidant	123:142	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	8	18	theme	disc	1448:1451	arg1	diffusion					1453:1461	disc diffusion	1448:1461	disc diffusion	1448:1461	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	5	19	theme	fucose	832:837	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	4	20	theme	monosaccharide	582:595	arg1	composition					597:607	monosaccharide composition	582:607	monosaccharide composition	582:607	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	10	21	from	platensis	1705:1713	arg1	SPs					1693:1695	the isolated SPs	1680:1695	the isolated SPs from S. platensis	1680:1713	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	10	22	theme	pharmaceutical	1723:1736	arg1	properties					1761:1770	pharmaceutical as well as nutritional properties	1723:1770	pharmaceutical as well as nutritional properties	1723:1770	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	5	23	theme	glucose	805:811	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	glucose					805:811	glucose	805:811	glucose	805:811	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	fucose					832:837	fucose	832:837	fucose	832:837	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	mannose					840:846	mannose	840:846	mannose	840:846	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	weight					877:882	the molecular weight	863:882	the molecular weight of SPs	863:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	rhamnose					814:821	rhamnose	814:821	rhamnose	814:821	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	xylose					824:829	xylose	824:829	xylose	824:829	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	24	theme	SPs	887:889	arg1	galactose					849:857	galactose	849:857	galactose	849:857	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	7	25	theme	potent	1061:1066	arg1	activity					1080:1087	potent antioxidant activity	1061:1087	potent antioxidant activity in DPPH (76.45 ± 0.49%)	1061:1111	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	9	26	theme	Danio	1584:1588	arg1	rerio					1590:1594	the Danio rerio	1580:1594	the Danio rerio	1580:1594	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	5	27	theme	galactose	849:857	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	8	28	theme	protein	1482:1488	arg1	leakage					1490:1496	protein leakage	1482:1496	protein leakage	1482:1496	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	7	29	theme	obtained	1038:1045	arg1	SPs					1047:1049	The obtained SPs	1034:1049	The obtained SPs	1034:1049	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	30	theme	peroxide	1164:1171	arg1	%					1196:1196	66.3 ± 1.16%	1185:1196	66.3 ± 1.16%	1185:1196	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	30	theme	peroxide	1164:1171	arg1	scavenging					1173:1182	hydrogen peroxide scavenging	1155:1182	hydrogen peroxide scavenging (66.3 ± 1.16%)	1155:1197	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	10	31	theme	S.	1702:1703	arg1	platensis					1705:1713	S. platensis	1702:1713	S. platensis	1702:1713	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	7	32	theme	hydroxyl	1200:1207	arg1	scavenging					1209:1218	hydroxyl scavenging	1200:1218	hydroxyl scavenging (68.6 ± 3.2%)	1200:1232	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	32	theme	hydroxyl	1200:1207	arg1	%					1231:1231	68.6 ± 3.2%	1221:1231	68.6 ± 3.2%	1221:1231	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	33	theme	nitric	1235:1240	arg1	oxide					1242:1246	nitric oxide	1235:1246	nitric oxide (81.36 ± 1.85%)	1235:1262	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	33	theme	nitric	1235:1240	arg1	%					1261:1261	81.36 ± 1.85%	1249:1261	81.36 ± 1.85%	1249:1261	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	5	34	theme	rhamnose	814:821	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	9	35	theme	2	1555:1555	arg1	%					1556:1556	%	1556:1556	%	1556:1556	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	4	36	theme	SPs	633:635	arg1	composition					597:607	monosaccharide composition	582:607	monosaccharide composition	582:607	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	4	36	theme	SPs	633:635	arg1	weight					623:628	molecular weight	613:628	molecular weight	613:628	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	1	37	theme	Spirulina	286:294	arg1	platensis					296:304	Spirulina platensis	286:304	Spirulina platensis	286:304	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	8	38	theme	highest	1364:1370	arg1	efficacy					1386:1393	the highest antibacterial efficacy	1360:1393	the highest antibacterial efficacy against the pathogenic bacteria	1360:1425	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	2	39	theme	isolated	311:318	arg1	SPs					320:322	The isolated SPs	307:322	The isolated SPs	307:322	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	6	40	theme	13C	1016:1018	arg1	NMR					1020:1022	13C NMR	1016:1022	13C NMR	1016:1022	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	0	41	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	8	42	theme	leakage	1490:1496	arg1	assays					1498:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	5	43	dep	showed	781:786	arg1	1016 kDa					895:902	1016 kDa	895:902	1016 kDa	895:902	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	4	44	theme	high-performance	654:669	arg1	chromatography					678:691	high-performance liquid chromatography	654:691	high-performance liquid chromatography	654:691	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	3	45	theme	elemental	456:464	arg1	analysis					466:473	The elemental analysis	452:473	The elemental analysis	452:473	The elemental analysis confirmed the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%).
31499110	0	46	theme	sulfated	45:52	arg1	polysaccharide					54:67	sulfated polysaccharide	45:67	sulfated polysaccharide	45:67	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	6	47	theme	SPs	938:940	arg1	characterization					918:933	the characterization	914:933	the characterization of SPs	914:940	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	0	48	theme	antibacterial	145:157	arg1	activity					159:166	antibacterial activity	145:166	antibacterial activity	145:166	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	7	49	theme	absorbance:1.66 ± 0.02	1287:1308	arg1	activities					1311:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	total antioxidant (absorbance:1.66 ± 0.02) activities	1268:1320	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	4	50	theme	molecular	613:621	arg1	weight					623:628	molecular weight	613:628	molecular weight	613:628	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	9	51	theme	%	1556:1556	arg1	SPs					1558:1560	2% SPs	1555:1560	2% SPs	1555:1560	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	0	52	from	platensis	84:92	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	52	from	platensis	84:92	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	10	53	contain	possess	1715:1721	arg2	properties					1761:1770	pharmaceutical as well as nutritional properties	1723:1770	pharmaceutical as well as nutritional properties	1723:1770	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	10	53	contain	possess	1715:1721	arg1	SPs					1693:1695	the isolated SPs	1680:1695	the isolated SPs from S. platensis	1680:1713	This finding confirmed that the isolated SPs from S. platensis possess pharmaceutical as well as nutritional properties.
31499110	5	54	theme	mannose	840:846	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	5	55	theme	molecular	867:875	arg1	weight					877:882	the molecular weight	863:882	the molecular weight of SPs	863:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	7	56	dep	absorbance	1130:1139	arg1	1.3 ± 0.02					1142:1151	1.3 ± 0.02	1142:1151	1.3 ± 0.02	1142:1151	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	5	57	theme	composition	760:770	arg1	analysis					772:779	The monosaccharide composition analysis	741:779	The monosaccharide composition analysis	741:779	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	4	58	dep	composition	597:607	arg1	The					578:580	The	578:580	The	578:580	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	8	59	theme	agar	1464:1467	arg1	bioassay					1469:1476	agar bioassay	1464:1476	agar bioassay	1464:1476	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	0	60	theme	polysaccharide	54:67	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	60	theme	polysaccharide	54:67	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	3	61	theme	nitrogen	553:560	arg1	presence					489:496	the presence	485:496	the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%)	485:575	The elemental analysis confirmed the presence of carbon (18.01 ± 0.10%), hydrogen (1.83 ± 0.02%) and nitrogen (3.43 ± 0.01%).
31499110	8	62	theme	antibacterial	1372:1384	arg1	efficacy					1386:1393	the highest antibacterial efficacy	1360:1393	the highest antibacterial efficacy against the pathogenic bacteria	1360:1425	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	2	63	contain	contains	324:331	arg1	%					394:394	38.7 ± 0.30%	383:394	38.7 ± 0.30%	383:394	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg1	%					421:421	7.1 ± 0.15%	411:421	7.1 ± 0.15%	411:421	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg1	SPs					320:322	The isolated SPs	307:322	The isolated SPs	307:322	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg2	carbohydrate					333:344	carbohydrate	333:344	carbohydrate	333:344	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg1	%					436:436	7.9 ± 0.4%	427:436	7.9 ± 0.4%	427:436	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg2	sulfate					347:353	sulfate	347:353	sulfate	347:353	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg1	%					408:408	21.3 ± 0.87%	397:408	21.3 ± 0.87%	397:408	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg2	SPs					320:322	The isolated SPs	307:322	The isolated SPs	307:322	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg2	protein					356:362	protein	356:362	protein	356:362	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	2	63	contain	contains	324:331	arg2	acid					375:378	uronic acid	368:378	uronic acid	368:378	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	9	64	theme	SPs	1558:1560	arg1	supplementation					1536:1550	the supplementation	1532:1550	the supplementation of 2% SPs through a feed to the Danio rerio fish	1532:1599	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	4	65	theme	exclusion	702:710	arg1	chromatography					712:725	size exclusion chromatography	697:725	size exclusion chromatography	697:725	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	0	66	theme	Zebrafish	172:180	arg1	growth					182:187	Zebrafish growth	172:187	Zebrafish growth	172:187	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	67	from	potential	112:120	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	67	from	potential	112:120	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	5	68	theme	xylose	824:829	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	4	69	theme	size	697:700	arg1	chromatography					712:725	size exclusion chromatography	697:725	size exclusion chromatography	697:725	The monosaccharide composition and molecular weight of SPs were analyzed by high-performance liquid chromatography and size exclusion chromatography respectively.
31499110	9	70	theme	feed	1572:1575	arg1	fish					1596:1599	a feed to the Danio rerio fish	1570:1599	a feed to the Danio rerio fish	1570:1599	Furthermore, the supplementation of 2% SPs through a feed to the Danio rerio fish enhances the growth and reproductive performances.
31499110	1	71	theme	sulfated	238:245	arg1	polysaccharide					247:260	the sulfated polysaccharide	234:260	the sulfated polysaccharide (SPs)	234:266	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	1	71	theme	sulfated	238:245	arg1	SPs					263:265	SPs	263:265	SPs	263:265	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	5	72	theme	monosaccharide	745:758	arg1	analysis					772:779	The monosaccharide composition analysis	741:779	The monosaccharide composition analysis	741:779	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	6	73	theme	1H	1005:1006	arg1	NMR					1008:1010	1H NMR	1005:1010	1H NMR	1005:1010	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	1	74	attach	isolated	272:279	arg1	platensis					296:304	Spirulina platensis	286:304	Spirulina platensis	286:304	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	1	74	attach	isolated	272:279	arg2	SPs					263:265	SPs	263:265	SPs	263:265	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	1	74	attach	isolated	272:279	arg2	polysaccharide					247:260	the sulfated polysaccharide	234:260	the sulfated polysaccharide (SPs)	234:266	In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis.
31499110	8	75	theme	bioassay	1469:1476	arg1	assays					1498:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	disc diffusion, agar bioassay and protein leakage assays	1448:1503	In addition, SPs revealed the highest antibacterial efficacy against the pathogenic bacteria Vibrio vulnificus in disc diffusion, agar bioassay and protein leakage assays at 100 μg/ml.
31499110	5	76	theme	weight	877:882	arg1	existence					792:800	the existence	788:800	the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs	788:889	The monosaccharide composition analysis showed the existence of glucose, rhamnose, xylose, fucose, mannose, galactose and the molecular weight of SPs was 1016 kDa.
31499110	0	77	theme	Spirulina	74:82	arg1	platensis					84:92	Spirulina platensis	74:92	Spirulina platensis	74:92	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	6	78	dep	spectroscopy	965:976	arg1	analysis					1024:1031	analysis	1024:1031	analysis	1024:1031	Further, the characterization of SPs was done by UV-visible spectroscopy, X-ray diffraction, FT-IR, 1H NMR and 13C NMR analysis.
31499110	0	79	dep	Isolation	0:8	arg1	antioxidant					132:142	In vitro antioxidant	123:142	In vitro antioxidant	123:142	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	79	dep	Isolation	0:8	arg1	activity					159:166	antibacterial activity	145:166	antibacterial activity	145:166	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	0	79	dep	Isolation	0:8	arg1	growth					182:187	Zebrafish growth	172:187	Zebrafish growth	172:187	Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance.
31499110	2	80	theme	uronic	368:373	arg1	acid					375:378	uronic acid	368:378	uronic acid	368:378	The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively.
31499110	7	81	from	activity	1080:1087	arg1	%					1110:1110	76.45 ± 0.49%	1098:1110	76.45 ± 0.49%	1098:1110	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31499110	7	81	from	activity	1080:1087	arg1	DPPH					1092:1095	DPPH	1092:1095	DPPH (76.45 ± 0.49%)	1092:1111	The obtained SPs exhibited potent antioxidant activity in DPPH (76.45 ± 0.49%), reducing power (absorbance: 1.3 ± 0.02), hydrogen peroxide scavenging (66.3 ± 1.16%), hydroxyl scavenging (68.6 ± 3.2%), nitric oxide (81.36 ± 1.85%) and total antioxidant (absorbance:1.66 ± 0.02) activities at 5 mg/ml.
31793675	0	0	theme	gut	104:106	arg1	composition					119:129	gut microbiota composition	104:129	gut microbiota composition in high fat diet-fed golden hamsters	104:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	6	1	theme	PRACTICAL	1318:1326	arg1	APPLICATIONS					1328:1339	PRACTICAL APPLICATIONS	1318:1339	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.	1318:1527	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	5	2	theme	above-mentioned	1078:1092	arg1	findings					1094:1101	the above-mentioned findings	1074:1101	the above-mentioned findings	1074:1101	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	5	3	theme	lipid-lowering	1271:1284	arg1	ingredient					1286:1295	a potential lipid-lowering ingredient	1259:1295	a potential lipid-lowering ingredient in functional foods	1259:1315	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	5	3	theme	lipid-lowering	1271:1284	arg1	PC					1239:1240	PC	1239:1240	PC	1239:1240	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	2	4	theme	serum	570:574	arg1	TG					596:597	TG	596:597	TG	596:597	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	4	theme	serum	570:574	arg1	triglyceride					582:593	serum total triglyceride	570:593	serum total triglyceride (TG)	570:598	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	1	5	theme	dysfunctional	356:368	arg1	barrier					381:387	dysfunctional intestinal barrier	356:387	dysfunctional intestinal barrier	356:387	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	2	6	theme	cholesterol	607:617	arg1	contents					558:565	the contents	554:565	the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST)	554:705	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	6	7	theme	novel	1476:1480	arg1	ingredient					1497:1506	a novel lipid-lowering ingredient	1474:1506	a novel lipid-lowering ingredient in functional foods	1474:1526	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	1	8	theme	nonalcoholic	262:273	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	1	8	theme	nonalcoholic	262:273	arg1	disease					287:293	nonalcoholic fatty liver disease	262:293	nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier	262:387	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	1	9	theme	intestinal	370:379	arg1	barrier					381:387	dysfunctional intestinal barrier	356:387	dysfunctional intestinal barrier	356:387	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	2	10	theme	total	601:605	arg1	cholesterol					607:617	total cholesterol	601:617	total cholesterol (TC)	601:622	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	10	theme	total	601:605	arg1	TC					620:621	TC	620:621	TC	620:621	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	5	11	from	ingredient	1286:1295	arg1	foods					1311:1315	functional foods	1300:1315	functional foods	1300:1315	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	1	12	theme	fatty	275:279	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	1	12	theme	fatty	275:279	arg1	disease					287:293	nonalcoholic fatty liver disease	262:293	nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier	262:387	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	0	13	theme	microbiota	108:117	arg1	composition					119:129	gut microbiota composition	104:129	gut microbiota composition in high fat diet-fed golden hamsters	104:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	2	14	theme	cholesterol	649:659	arg1	contents					558:565	the contents	554:565	the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST)	554:705	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	1	15	theme	liver	281:285	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	1	15	theme	liver	281:285	arg1	disease					287:293	nonalcoholic fatty liver disease	262:293	nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier	262:387	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	0	16	theme	high	134:137	arg1	hamsters					159:166	high fat diet-fed golden hamsters	134:166	high fat diet-fed golden hamsters	134:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	2	17	theme	low-density	625:635	arg1	LDL-C					662:666	LDL-C	662:666	LDL-C	662:666	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	17	theme	low-density	625:635	arg1	cholesterol					649:659	low-density lipoprotein cholesterol	625:659	low-density lipoprotein cholesterol (LDL-C)	625:667	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	18	theme	lipoprotein	637:647	arg1	LDL-C					662:666	LDL-C	662:666	LDL-C	662:666	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	18	theme	lipoprotein	637:647	arg1	cholesterol					649:659	low-density lipoprotein cholesterol	625:659	low-density lipoprotein cholesterol (LDL-C)	625:667	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	7	19	theme	metabolic	1727:1735	arg1	MetS					1747:1750	MetS	1747:1750	MetS	1747:1750	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	7	19	theme	metabolic	1727:1735	arg1	syndrome					1737:1744	metabolic syndrome	1727:1744	metabolic syndrome (MetS)	1727:1751	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	3	20	theme	bacteria	830:837	arg1	abundances					805:814	the relative abundances	792:814	the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers	792:892	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	5	21	used	used	1251:1254	arg2	ingredient					1286:1295	a potential lipid-lowering ingredient	1259:1295	a potential lipid-lowering ingredient in functional foods	1259:1315	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	5	21	used	used	1251:1254	arg2	PC					1239:1240	PC	1239:1240	PC	1239:1240	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	2	22	theme	aspartate	674:682	arg1	AST					702:704	AST	702:704	AST	702:704	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	22	theme	aspartate	674:682	arg1	aminotransferase					684:699	aspartate aminotransferase	674:699	aspartate aminotransferase (AST)	674:705	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	6	23	dep	APPLICATIONS	1328:1339	arg1	ameliorate					1351:1360	ameliorate	1351:1360	ameliorate	1351:1360	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	4	24	theme	Alloprevotella	959:972	arg1	abundances					933:942	the abundances	929:942	the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes	929:1004	Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles.
31793675	4	25	theme	serum	1038:1042	arg1	profiles					1050:1057	serum lipid profiles	1038:1057	serum lipid profiles	1038:1057	Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles.
31793675	5	26	theme	lipid	1135:1139	arg1	disorders					1151:1159	lipid metabolic disorders	1135:1159	lipid metabolic disorders	1135:1159	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	6	27	theme	lipid-lowering	1482:1495	arg1	ingredient					1497:1506	a novel lipid-lowering ingredient	1474:1506	a novel lipid-lowering ingredient in functional foods	1474:1526	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	4	28	theme	Bifidobacterium	975:989	arg1	abundances					933:942	the abundances	929:942	the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes	929:1004	Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles.
31793675	6	29	theme	gut	1430:1432	arg1	microbiota					1434:1443	specific gut microbiota	1421:1443	specific gut microbiota	1421:1443	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	0	30	theme	Ganoderma	0:8	arg1	polysaccharide					10:23	Ganoderma polysaccharide	0:23	Ganoderma polysaccharide	0:23	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	6	31	theme	metabolism	1368:1377	arg1	disorder					1379:1386	lipid metabolism disorder	1362:1386	lipid metabolism disorder	1362:1386	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	1	32	theme	sucrose	193:199	arg1	intake					201:206	sucrose intake	193:206	sucrose intake	193:206	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	0	33	theme	diet-fed	143:150	arg1	hamsters					159:166	high fat diet-fed golden hamsters	134:166	high fat diet-fed golden hamsters	134:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	2	34	theme	lucidum	437:443	arg1	polysaccharide					445:458	Ganoderma lucidum polysaccharide	427:458	Ganoderma lucidum polysaccharide	427:458	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	4	35	theme	lipid	1044:1048	arg1	profiles					1050:1057	serum lipid profiles	1038:1057	serum lipid profiles	1038:1057	Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles.
31793675	7	36	theme	microbiota	1647:1656	arg1	alterations					1658:1668	the gut microbiota alterations	1639:1668	the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS)	1639:1751	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	0	37	theme	fat	139:141	arg1	hamsters					159:166	high fat diet-fed golden hamsters	134:166	high fat diet-fed golden hamsters	134:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	2	38	from	hyperlipidemia	506:519	arg1	hamsters					532:539	HFD-fed hamsters	524:539	HFD-fed hamsters	524:539	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	7	39	theme	PC	1690:1691	arg1	supplementation					1693:1707	PC supplementation	1690:1707	PC supplementation	1690:1707	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	2	40	theme	Ganoderma	427:435	arg1	lucidum					437:443	Ganoderma lucidum	427:443	Ganoderma lucidum polysaccharide	427:458	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	6	41	theme	lipid	1362:1366	arg1	disorder					1379:1386	lipid metabolism disorder	1362:1386	lipid metabolism disorder	1362:1386	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	7	42	theme	syndrome	1737:1744	arg1	context					1716:1722	the context	1712:1722	the context of metabolic syndrome (MetS)	1712:1751	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	2	43	theme	present	397:403	arg1	study					405:409	the present study	393:409	the present study	393:409	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	4	44	theme	Alistipes	996:1004	arg1	abundances					933:942	the abundances	929:942	the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes	929:1004	Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles.
31793675	0	45	theme	golden	152:157	arg1	hamsters					159:166	high fat diet-fed golden hamsters	134:166	high fat diet-fed golden hamsters	134:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	3	46	theme	microbiota	768:777	arg1	composition					736:746	the composition	732:746	the composition of gastrointestinal microbiota	732:777	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	0	47	theme	metabolic	71:79	arg1	disorders					81:89	lipid metabolic disorders	65:89	lipid metabolic disorders	65:89	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	7	48	theme	gut	1643:1645	arg1	alterations					1658:1668	the gut microbiota alterations	1639:1668	the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS)	1639:1751	We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).
31793675	0	49	theme	lipid	65:69	arg1	disorders					81:89	lipid metabolic disorders	65:89	lipid metabolic disorders	65:89	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	3	50	theme	gastrointestinal	751:766	arg1	microbiota					768:777	gastrointestinal microbiota	751:777	gastrointestinal microbiota	751:777	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	2	51	theme	total	576:580	arg1	TG					596:597	TG	596:597	TG	596:597	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	51	theme	total	576:580	arg1	triglyceride					582:593	serum total triglyceride	570:593	serum total triglyceride (TG)	570:598	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	5	52	theme	functional	1300:1309	arg1	foods					1311:1315	functional foods	1300:1315	functional foods	1300:1315	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	1	53	theme	disturbed	314:322	arg1	gastrointestinal microbiota					324:350	disturbed gastrointestinal microbiota	314:350	disturbed gastrointestinal microbiota	314:350	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	2	54	theme	aminotransferase	684:699	arg1	contents					558:565	the contents	554:565	the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST)	554:705	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	2	55	theme	triglyceride	582:593	arg1	contents					558:565	the contents	554:565	the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST)	554:705	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	0	56	from	composition	119:129	arg1	hamsters					159:166	high fat diet-fed golden hamsters	134:166	high fat diet-fed golden hamsters	134:166	Ganoderma polysaccharide and chitosan synergistically ameliorate lipid metabolic disorders and modulate gut microbiota composition in high fat diet-fed golden hamsters.
31793675	6	57	theme	functional	1511:1520	arg1	foods					1522:1526	functional foods	1511:1526	functional foods	1511:1526	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	2	58	theme	HFD-fed	524:530	arg1	hamsters					532:539	HFD-fed hamsters	524:539	HFD-fed hamsters	524:539	In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST).
31793675	6	59	from	ingredient	1497:1506	arg1	foods					1522:1526	functional foods	1511:1526	functional foods	1511:1526	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	6	60	theme	specific	1421:1428	arg1	microbiota					1434:1443	specific gut microbiota	1421:1443	specific gut microbiota	1421:1443	PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods.
31793675	3	61	theme	beneficial	819:828	arg1	bacteria					830:837	beneficial bacteria	819:837	beneficial bacteria	819:837	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	3	61	theme	beneficial	819:828	arg1	Oscillibacter					860:872	Oscillibacter	860:872	Oscillibacter	860:872	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	3	61	theme	beneficial	819:828	arg1	SCFA-producers					879:892	SCFA-producers	879:892	SCFA-producers	879:892	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	3	61	theme	beneficial	819:828	arg1	Prevotella					848:857	Prevotella	848:857	Prevotella	848:857	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
31793675	5	62	theme	metabolic	1141:1149	arg1	disorders					1151:1159	lipid metabolic disorders	1135:1159	lipid metabolic disorders	1135:1159	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	1	63	theme	High-fat	169:176	arg1	HFD					184:186	HFD	184:186	HFD	184:186	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	1	63	theme	High-fat	169:176	arg1	diet					178:181	High-fat diet	169:181	High-fat diet (HFD)	169:187	High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier.
31793675	5	64	theme	potential	1261:1269	arg1	ingredient					1286:1295	a potential lipid-lowering ingredient	1259:1295	a potential lipid-lowering ingredient in functional foods	1259:1315	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	5	64	theme	potential	1261:1269	arg1	PC					1239:1240	PC	1239:1240	PC	1239:1240	Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods.
31793675	3	65	theme	relative	796:803	arg1	abundances					805:814	the relative abundances	792:814	the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers	792:892	Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers.
29729339	8	0	from	death	1180:1184	arg1	cells					1207:1211	F2 treated cancer cells	1189:1211	F2 treated cancer cells	1189:1211	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	1	1	theme	anticancer	153:162	arg1	potential					164:172	the antioxidant and anticancer potential	133:172	the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum	133:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	8	2	theme	confocal	1068:1075	arg1	analysis					1104:1111	The fluorescence and confocal laser scanning microscopic analysis	1047:1111	analysis	1104:1111	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	4	3	theme	sulphate	521:528	arg1	content					530:536	sulphate content	521:536	sulphate content	521:536	Among them, fraction 2 (F2) showed the higher yield percentage, fucose and sulphate content.
29729339	7	4	theme	HCT-15	927:932	arg1	lines					939:943	HCT-15 cell lines	927:943	HCT-15 cell lines	927:943	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	6	5	theme	nitric	823:828	arg1	oxide					830:834	nitric oxide	823:834	nitric oxide radical scavenging activity (51.81 ± 1.04%)	823:878	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	9	6	located	found	1247:1251	arg1	cells					1278:1282	the F2 treated cancer cells	1256:1282	the F2 treated cancer cells	1256:1282	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	9	6	located	found	1247:1251	arg2	release					1235:1241	LDH release	1231:1241	LDH release	1231:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	9	6	located	found	1247:1251	arg2	amount					1221:1226	Higher amount	1214:1226	Higher amount of LDH release	1214:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	8	7	theme	morphological	1140:1152	arg1	changes					1154:1160	the apoptotic morphological changes	1126:1160	the apoptotic morphological changes	1126:1160	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	0	8	from	Investigation	0:12	arg1	polycystum					81:90	Sargassum polycystum	71:90	Sargassum polycystum	71:90	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	7	9	theme	cell	934:937	arg1	lines					939:943	HCT-15 cell lines	927:943	HCT-15 cell lines	927:943	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	5	10	theme	F2	620:621	arg1	properties					602:611	structural and functional properties	576:611	structural and functional properties of the F2	576:621	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	5	10	theme	F2	620:621	arg1	composition					563:573	monosaccharide composition	548:573	monosaccharide composition	548:573	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	9	11	theme	treated	1263:1269	arg1	cells					1278:1282	the F2 treated cancer cells	1256:1282	the F2 treated cancer cells	1256:1282	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	8	12	theme	apoptotic	1130:1138	arg1	changes					1154:1160	the apoptotic morphological changes	1126:1160	the apoptotic morphological changes	1126:1160	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	4	13	theme	higher	485:490	arg1	yield					492:496	the higher yield	481:496	the higher yield percentage	481:507	Among them, fraction 2 (F2) showed the higher yield percentage, fucose and sulphate content.
29729339	6	14	theme	scavenging	783:792	arg1	activity					794:801	hydrogen peroxide scavenging activity	765:801	hydrogen peroxide scavenging activity (71.76 ± 2.14%)	765:817	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	14	theme	scavenging	783:792	arg1	%					816:816	71.76 ± 2.14%	804:816	71.76 ± 2.14%	804:816	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	8	15	theme	microscopic	1092:1102	arg1	analysis					1104:1111	The fluorescence and confocal laser scanning microscopic analysis	1047:1111	analysis	1104:1111	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	6	16	theme	peroxide	774:781	arg1	activity					794:801	hydrogen peroxide scavenging activity	765:801	hydrogen peroxide scavenging activity (71.76 ± 2.14%)	765:817	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	16	theme	peroxide	774:781	arg1	%					816:816	71.76 ± 2.14%	804:816	71.76 ± 2.14%	804:816	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	8	17	theme	treated	1192:1198	arg1	cells					1207:1211	F2 treated cancer cells	1189:1211	F2 treated cancer cells	1189:1211	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	6	18	theme	hydrogen	765:772	arg1	peroxide					774:781	hydrogen peroxide	765:781	hydrogen peroxide scavenging activity (71.76 ± 2.14%)	765:817	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	2	19	theme	exchange	292:299	arg1	chromatography					301:314	DEAE cellulose-ion exchange chromatography	273:314	DEAE cellulose-ion exchange chromatography	273:314	The isolated fucoidan was successfully purified by DEAE cellulose-ion exchange chromatography and dialysis.
29729339	1	20	theme	fucoidan	177:184	arg1	potential					164:172	the antioxidant and anticancer potential	133:172	the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum	133:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	7	21	theme	MCF-7	917:921	arg1	viability					904:912	The cell viability	895:912	The cell viability of MCF-7 and HCT-15 cell lines	895:943	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	5	22	theme	functional	591:600	arg1	properties					602:611	structural and functional properties	576:611	structural and functional properties of the F2	576:621	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	5	22	theme	functional	591:600	arg1	composition					563:573	monosaccharide composition	548:573	monosaccharide composition	548:573	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	9	23	theme	cancer	1271:1276	arg1	cells					1278:1282	the F2 treated cancer cells	1256:1282	the F2 treated cancer cells	1256:1282	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	6	24	theme	scavenging	689:698	arg1	activity					700:707	highest DPPH radical scavenging activity	668:707	highest DPPH radical scavenging activity (55.94 ± 0.69%)	668:723	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	24	theme	scavenging	689:698	arg1	%					722:722	55.94 ± 0.69%	710:722	55.94 ± 0.69%	710:722	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	8	25	theme	cell	1166:1169	arg1	death					1180:1184	cell mediated death	1166:1184	cell mediated death	1166:1184	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	9	26	theme	control	1293:1299	arg1	group					1301:1305	the control group	1289:1305	the control group	1289:1305	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	1	27	theme	present	97:103	arg1	study					105:109	The present study	93:109	The present study	93:109	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	6	28	theme	radical	681:687	arg1	activity					700:707	highest DPPH radical scavenging activity	668:707	highest DPPH radical scavenging activity (55.94 ± 0.69%)	668:723	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	28	theme	radical	681:687	arg1	%					722:722	55.94 ± 0.69%	710:722	55.94 ± 0.69%	710:722	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	29	theme	radical	836:842	arg1	activity					855:862	nitric oxide radical scavenging activity	823:862	nitric oxide radical scavenging activity (51.81 ± 1.04%)	823:878	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	29	theme	radical	836:842	arg1	%					877:877	51.81 ± 1.04%	865:877	51.81 ± 1.04%	865:877	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	4	30	dep	yield	492:496	arg1	percentage					498:507	percentage	498:507	the higher yield percentage	481:507	Among them, fraction 2 (F2) showed the higher yield percentage, fucose and sulphate content.
29729339	10	31	theme	anticancer	1402:1411	arg1	property					1413:1420	anticancer property	1402:1420	anticancer property	1402:1420	Thus, the present finding proved that the isolated F2 encompasses significant antioxidant and anticancer property.
29729339	7	32	theme	cell	899:902	arg1	viability					904:912	The cell viability	895:912	The cell viability of MCF-7 and HCT-15 cell lines	895:943	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	6	33	theme	DPPH	676:679	arg1	activity					700:707	highest DPPH radical scavenging activity	668:707	highest DPPH radical scavenging activity (55.94 ± 0.69%)	668:723	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	33	theme	DPPH	676:679	arg1	%					722:722	55.94 ± 0.69%	710:722	55.94 ± 0.69%	710:722	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	1	34	theme	Sargassum	200:208	arg1	polycystum					210:219	Sargassum polycystum	200:219	Sargassum polycystum	200:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	3	35	theme	chemical	400:407	arg1	constitution					409:420	its chemical constitution	396:420	its chemical constitution	396:420	Totally four active fractions (F1-F4) were collected and explored its chemical constitution by calorimetric assays.
29729339	6	36	theme	scavenging	844:853	arg1	activity					855:862	nitric oxide radical scavenging activity	823:862	nitric oxide radical scavenging activity (51.81 ± 1.04%)	823:878	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	36	theme	scavenging	844:853	arg1	%					877:877	51.81 ± 1.04%	865:877	51.81 ± 1.04%	865:877	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	37	theme	highest	668:674	arg1	activity					700:707	highest DPPH radical scavenging activity	668:707	highest DPPH radical scavenging activity (55.94 ± 0.69%)	668:723	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	37	theme	highest	668:674	arg1	%					722:722	55.94 ± 0.69%	710:722	55.94 ± 0.69%	710:722	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	10	38	theme	significant	1374:1384	arg1	antioxidant					1386:1396	significant antioxidant	1374:1396	significant antioxidant	1374:1396	Thus, the present finding proved that the isolated F2 encompasses significant antioxidant and anticancer property.
29729339	0	39	theme	potential	44:52	arg1	Investigation					0:12	Investigation	0:12	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.	0:91	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	10	40	theme	isolated	1350:1357	arg1	F2					1359:1360	the isolated F2	1346:1360	the isolated F2	1346:1360	Thus, the present finding proved that the isolated F2 encompasses significant antioxidant and anticancer property.
29729339	0	41	from	polycystum	81:90	arg1	potential					44:52	antioxidant and anticancer potential	17:52	antioxidant and anticancer potential of fucoidan from Sargassum polycystum	17:90	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	0	41	from	polycystum	81:90	arg1	Investigation					0:12	Investigation	0:12	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.	0:91	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	0	42	theme	anticancer	33:42	arg1	potential					44:52	antioxidant and anticancer potential	17:52	antioxidant and anticancer potential of fucoidan from Sargassum polycystum	17:90	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	8	43	theme	F2	1189:1190	arg1	cells					1207:1211	F2 treated cancer cells	1189:1211	F2 treated cancer cells	1189:1211	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	6	44	theme	absorbance	747:756	arg1	rate					758:761	0.33 absorbance rate	742:761	0.33 absorbance rate	742:761	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	44	theme	absorbance	747:756	arg1	power					735:739	reducing power	726:739	reducing power (0.33 absorbance rate)	726:762	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	5	45	theme	monosaccharide	548:561	arg1	properties					602:611	structural and functional properties	576:611	structural and functional properties of the F2	576:621	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	5	45	theme	monosaccharide	548:561	arg1	composition					563:573	monosaccharide composition	548:573	monosaccharide composition	548:573	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	0	46	theme	fucoidan	57:64	arg1	potential					44:52	antioxidant and anticancer potential	17:52	antioxidant and anticancer potential of fucoidan from Sargassum polycystum	17:90	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	7	47	theme	estimated	998:1006	arg1	IC50					1008:1011	an estimated IC50	995:1011	an estimated IC50 was 20 and 50 μg/ml respectively	995:1044	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	1	48	attach	isolated	186:193	arg1	polycystum					210:219	Sargassum polycystum	200:219	Sargassum polycystum	200:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	1	48	attach	isolated	186:193	arg2	fucoidan					177:184	fucoidan	177:184	fucoidan isolated from Sargassum polycystum	177:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	7	49	theme	F2	987:988	arg1	concentration					970:982	the concentration	966:982	the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively	966:1044	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	8	50	theme	cancer	1200:1205	arg1	cells					1207:1211	F2 treated cancer cells	1189:1211	F2 treated cancer cells	1189:1211	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	3	51	theme	active	343:348	arg1	F1-F4					361:365	F1-F4	361:365	F1-F4	361:365	Totally four active fractions (F1-F4) were collected and explored its chemical constitution by calorimetric assays.
29729339	3	51	theme	active	343:348	arg1	fractions					350:358	four active fractions	338:358	four active fractions (F1-F4)	338:366	Totally four active fractions (F1-F4) were collected and explored its chemical constitution by calorimetric assays.
29729339	9	52	theme	LDH	1231:1233	arg1	release					1235:1241	LDH release	1231:1241	LDH release	1231:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	2	53	theme	isolated	226:233	arg1	fucoidan					235:242	The isolated fucoidan	222:242	The isolated fucoidan	222:242	The isolated fucoidan was successfully purified by DEAE cellulose-ion exchange chromatography and dialysis.
29729339	6	54	theme	oxide	830:834	arg1	activity					855:862	nitric oxide radical scavenging activity	823:862	nitric oxide radical scavenging activity (51.81 ± 1.04%)	823:878	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	54	theme	oxide	830:834	arg1	%					877:877	51.81 ± 1.04%	865:877	51.81 ± 1.04%	865:877	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	8	55	theme	scanning	1083:1090	arg1	analysis					1104:1111	The fluorescence and confocal laser scanning microscopic analysis	1047:1111	analysis	1104:1111	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	8	56	from	changes	1154:1160	arg1	cells					1207:1211	F2 treated cancer cells	1189:1211	F2 treated cancer cells	1189:1211	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	2	57	theme	cellulose-ion	278:290	arg1	chromatography					301:314	DEAE cellulose-ion exchange chromatography	273:314	DEAE cellulose-ion exchange chromatography	273:314	The isolated fucoidan was successfully purified by DEAE cellulose-ion exchange chromatography and dialysis.
29729339	5	58	theme	structural	576:585	arg1	properties					602:611	structural and functional properties	576:611	structural and functional properties of the F2	576:621	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	5	58	theme	structural	576:585	arg1	composition					563:573	monosaccharide composition	548:573	monosaccharide composition	548:573	Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR.
29729339	8	59	theme	laser	1077:1081	arg1	analysis					1104:1111	The fluorescence and confocal laser scanning microscopic analysis	1047:1111	analysis	1104:1111	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
29729339	2	60	theme	DEAE	273:276	arg1	chromatography					301:314	DEAE cellulose-ion exchange chromatography	273:314	DEAE cellulose-ion exchange chromatography	273:314	The isolated fucoidan was successfully purified by DEAE cellulose-ion exchange chromatography and dialysis.
29729339	6	61	theme	reducing	726:733	arg1	rate					758:761	0.33 absorbance rate	742:761	0.33 absorbance rate	742:761	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	6	61	theme	reducing	726:733	arg1	power					735:739	reducing power	726:739	reducing power (0.33 absorbance rate)	726:762	F2 shows highest DPPH radical scavenging activity (55.94 ± 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 ± 2.14%) and nitric oxide radical scavenging activity (51.81 ± 1.04%) at 1000 μg/ml.
29729339	9	62	theme	Higher	1214:1219	arg1	amount					1221:1226	Higher amount	1214:1226	Higher amount of LDH release	1214:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	9	62	theme	Higher	1214:1219	arg1	release					1235:1241	LDH release	1231:1241	LDH release	1231:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	10	63	theme	present	1318:1324	arg1	finding					1326:1332	the present finding	1314:1332	the present finding	1314:1332	Thus, the present finding proved that the isolated F2 encompasses significant antioxidant and anticancer property.
29729339	1	64	theme	antioxidant	137:147	arg1	potential					164:172	the antioxidant and anticancer potential	133:172	the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum	133:219	The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum.
29729339	9	65	theme	release	1235:1241	arg1	amount					1221:1226	Higher amount	1214:1226	Higher amount of LDH release	1214:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	9	65	theme	release	1235:1241	arg1	release					1235:1241	LDH release	1231:1241	LDH release	1231:1241	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	3	66	theme	calorimetric	425:436	arg1	assays					438:443	calorimetric assays	425:443	calorimetric assays	425:443	Totally four active fractions (F1-F4) were collected and explored its chemical constitution by calorimetric assays.
29729339	9	67	theme	F2	1260:1261	arg1	cells					1278:1282	the F2 treated cancer cells	1256:1282	the F2 treated cancer cells	1256:1282	Higher amount of LDH release was found in the F2 treated cancer cells than the control group.
29729339	0	68	theme	antioxidant	17:27	arg1	potential					44:52	antioxidant and anticancer potential	17:52	antioxidant and anticancer potential of fucoidan from Sargassum polycystum	17:90	Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum.
29729339	7	69	theme	lines	939:943	arg1	viability					904:912	The cell viability	895:912	The cell viability of MCF-7 and HCT-15 cell lines	895:943	The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 μg/ml respectively.
29729339	8	70	theme	mediated	1171:1178	arg1	death					1180:1184	cell mediated death	1166:1184	cell mediated death	1166:1184	The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells.
30342121	0	0	from	Laminaria	21:29	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Laminaria japonica	0:38	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30342121	2	1	theme	diet-induced	351:362	arg1	obesity					364:370	diet-induced obesity	351:370	diet-induced obesity in a mice model	351:386	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	2	theme	adipose	469:475	arg1	tissues					477:483	the liver and adipose tissues	455:483	tissues	477:483	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	3	from	accumulation	439:450	arg1	tissues					477:483	the liver and adipose tissues	455:483	tissues	477:483	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	3	4	theme	gut	613:615	arg1	microbiota					617:626	the gut microbiota	609:626	the gut microbiota	609:626	Such effects were associated with the modulation of the gut microbiota, involving some members of the Bacteroidetes phylum.
30342121	1	5	theme	LJPs	253:256	arg1	mechanisms					239:248	mechanisms	239:248	mechanisms	239:248	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	1	5	theme	LJPs	253:256	arg1	effects					227:233	effects	227:233	effects	227:233	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	0	6	theme	gut	104:106	arg1	microbiota					108:117	the gut microbiota	100:117	the gut microbiota	100:117	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30342121	3	7	theme	Bacteroidetes	659:671	arg1	members					644:650	some members	639:650	some members of the Bacteroidetes phylum	639:678	Such effects were associated with the modulation of the gut microbiota, involving some members of the Bacteroidetes phylum.
30342121	1	8	dep	Laminaria	141:149	arg1	japonica					151:158	Laminaria japonica	141:158	Laminaria japonica (LJPs)	141:165	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	5	9	theme	syndrome	957:964	arg1	health					984:989	the human gut health	970:989	the human gut health	970:989	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	5	9	theme	syndrome	957:964	arg1	improvement					932:942	the improvement	928:942	the improvement of metabolic syndrome	928:964	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	2	10	theme	lipids	504:509	arg1	profile					511:517	lipids profile	504:517	lipids profile	504:517	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	0	11	from	syndrome	61:68	arg1	mice					80:83	BALB/c mice	73:83	BALB/c mice	73:83	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30342121	4	12	theme	microbial	756:764	arg1	metabolites					766:776	microbial metabolites	756:776	microbial metabolites production	756:787	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	1	13	from	Laminaria	141:149	arg1	Polysaccharides					120:134	Polysaccharides	120:134	Polysaccharides from Laminaria japonica (LJPs)	120:165	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	4	14	theme	metabolites	766:776	arg1	production					778:787	microbial metabolites production	756:787	microbial metabolites production	756:787	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	2	15	dep	accumulation	439:450	arg1	morphology					528:537	the morphology	524:537	the morphology of the intestine	524:554	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	15	dep	accumulation	439:450	arg1	e.g.					430:433	e.g.	430:433	e.g.	430:433	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	15	dep	accumulation	439:450	arg1	composition					491:501	body composition	486:501	body composition	486:501	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	15	dep	accumulation	439:450	arg1	profile					511:517	lipids profile	504:517	lipids profile	504:517	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	1	16	from	mechanisms	239:248	arg1	diseases					285:292	its related diseases	273:292	its related diseases	273:292	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	1	16	from	mechanisms	239:248	arg1	obesity					261:267	obesity	261:267	obesity	261:267	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	2	17	theme	mice	377:380	arg1	model					382:386	a mice model	375:386	a mice model	375:386	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	18	theme	fat	435:437	arg1	accumulation					439:450	fat accumulation	435:450	fat accumulation in the liver and adipose tissues	435:483	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	1	19	theme	various	188:194	arg1	properties					207:216	various functional properties	188:216	various functional properties	188:216	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	3	20	dep	Bacteroidetes	659:671	arg1	phylum					673:678	phylum	673:678	phylum	673:678	Such effects were associated with the modulation of the gut microbiota, involving some members of the Bacteroidetes phylum.
30342121	2	21	from	obesity	364:370	arg1	model					382:386	a mice model	375:386	a mice model	375:386	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	4	22	theme	nutrient	725:732	arg1	utilization					734:744	nutrient utilization	725:744	nutrient utilization	725:744	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	1	23	theme	functional	196:205	arg1	properties					207:216	various functional properties	188:216	various functional properties	188:216	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	1	24	theme	related	277:283	arg1	diseases					285:292	its related diseases	273:292	its related diseases	273:292	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	3	25	theme	microbiota	617:626	arg1	modulation					595:604	the modulation	591:604	the modulation of the gut microbiota	591:626	Such effects were associated with the modulation of the gut microbiota, involving some members of the Bacteroidetes phylum.
30342121	2	26	theme	obesity-related	402:416	arg1	parameters					418:427	obesity-related parameters	402:427	obesity-related parameters	402:427	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	0	27	theme	metabolic	51:59	arg1	syndrome					61:68	metabolic syndrome	51:68	metabolic syndrome in BALB/c mice	51:83	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30342121	1	28	contain	have	183:186	arg1	Polysaccharides					120:134	Polysaccharides	120:134	Polysaccharides from Laminaria japonica (LJPs)	120:165	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	1	28	contain	have	183:186	arg2	properties					207:216	various functional properties	188:216	various functional properties	188:216	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	5	29	theme	human	974:978	arg1	health					984:989	the human gut health	970:989	the human gut health	970:989	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	2	30	theme	liver	459:463	arg1	tissues					477:483	the liver and adipose tissues	455:483	tissues	477:483	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	5	31	theme	gut	980:982	arg1	health					984:989	the human gut health	970:989	the human gut health	970:989	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	4	32	from	metabolism	815:824	arg1	body					833:836	the body	829:836	the body	829:836	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	1	33	dep	effects	227:233	arg1	the					223:225	the	223:225	the	223:225	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	3	34	theme	Such	557:560	arg1	effects					562:568	Such effects	557:568	Such effects	557:568	Such effects were associated with the modulation of the gut microbiota, involving some members of the Bacteroidetes phylum.
30342121	2	35	theme	intestine	546:554	arg1	morphology					528:537	the morphology	524:537	the morphology of the intestine	524:554	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	35	theme	intestine	546:554	arg1	composition					491:501	body composition	486:501	body composition	486:501	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	2	35	theme	intestine	546:554	arg1	profile					511:517	lipids profile	504:517	lipids profile	504:517	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	4	36	theme	lipids	808:813	arg1	metabolism					815:824	lipids metabolism	808:824	lipids metabolism in the body	808:836	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	4	37	dep	improve	717:723	arg1	regulate					799:806	regulate	799:806	regulate lipids metabolism in the body	799:836	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	5	38	theme	metabolic	947:955	arg1	syndrome					957:964	metabolic syndrome	947:964	metabolic syndrome	947:964	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	4	39	theme	gut	696:698	arg1	microbiota					700:709	the gut microbiota	692:709	the gut microbiota	692:709	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	5	40	theme	food	908:911	arg1	japonica					879:886	L. japonica	876:886	L. japonica	876:886	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	5	40	theme	food	908:911	arg1	supplement					913:922	food supplement	908:922	food supplement for the improvement of metabolic syndrome and the human gut health	908:989	In short, it sought to indicate that L. japonica can be developed as food supplement for the improvement of metabolic syndrome and the human gut health.
30342121	1	41	from	effects	227:233	arg1	diseases					285:292	its related diseases	273:292	its related diseases	273:292	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	1	41	from	effects	227:233	arg1	obesity					261:267	obesity	261:267	obesity	261:267	Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear.
30342121	0	42	dep	Laminaria	21:29	arg1	japonica					31:38	Laminaria japonica	21:38	Laminaria japonica	21:38	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30342121	2	43	theme	body	486:489	arg1	composition					491:501	body composition	486:501	body composition	486:501	This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related parameters, e.g. fat accumulation in the liver and adipose tissues, body composition, lipids profile, and the morphology of the intestine.
30342121	4	44	from	Changes	681:687	arg1	microbiota					700:709	the gut microbiota	692:709	the gut microbiota	692:709	Changes in the gut microbiota could improve nutrient utilization, increase microbial metabolites production, and thus regulate lipids metabolism in the body.
30342121	0	45	theme	BALB/c	73:78	arg1	mice					80:83	BALB/c mice	73:83	BALB/c mice	73:83	Polysaccharides from Laminaria japonica alleviated metabolic syndrome in BALB/c mice by normalizing the gut microbiota.
30559205	4	0	theme	protein	916:922	arg1	expression					884:893	expression	884:893	expression of the corresponding protein	884:922	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	3	1	dep	gut	574:576	arg1	requires					651:658	requires	651:658	requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization	651:760	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	1	2	theme	environmental	137:149	arg1	diet					178:181	the host diet	169:181	the host diet	169:181	The composition of the gut microbiota is largely determined by environmental factors including the host diet.
30559205	1	2	theme	environmental	137:149	arg1	factors					151:157	environmental factors	137:157	environmental factors including the host diet	137:181	The composition of the gut microbiota is largely determined by environmental factors including the host diet.
30559205	5	3	theme	complex	1152:1158	arg1	diet					1180:1183	a complex polysaccharide-rich diet	1150:1183	a complex polysaccharide-rich diet	1150:1183	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	4	4	theme	corresponding	902:914	arg1	protein					916:922	the corresponding protein	898:922	the corresponding protein	898:922	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	2	5	theme	Dietary	184:190	arg1	components					192:201	Dietary components	184:201	Dietary components	184:201	Dietary components are believed to influence the composition of the gut microbiota by serving as nutrients to a subset of microbes, thereby favoring their expansion.
30559205	5	6	theme	polysaccharide-rich	1160:1178	arg1	diet					1180:1183	a complex polysaccharide-rich diet	1150:1183	a complex polysaccharide-rich diet	1150:1183	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	7	theme	wild-type	1002:1010	arg1	thetaiotaomicron					1015:1030	wild-type B. thetaiotaomicron	1002:1030	wild-type B. thetaiotaomicron	1002:1030	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	8	dep	glucose	1059:1065	arg1	disaccharide					1082:1093	a disaccharide	1080:1093	a disaccharide composed of glucose and fructose	1080:1126	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	6	9	theme	beneficial	1341:1350	arg1	microbes					1352:1359	beneficial microbes	1341:1359	beneficial microbes independently of supplying nutrients to the microbiota	1341:1414	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	3	10	theme	colonization	749:760	arg1	regulator					736:744	regulator	736:744	regulator of colonization	736:760	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	6	11	theme	host	1266:1269	arg1	intestine					1271:1279	the host intestine	1262:1279	the host intestine	1262:1279	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	3	12	theme	leader	667:672	arg1	region					674:679	the 5' leader region	660:679	the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization	660:760	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	3	13	theme	gut	502:504	arg1	colonization					506:517	gut colonization	502:517	gut colonization	502:517	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	4	14	theme	roc	784:786	arg1	mRNA					795:798	the roc leader mRNA	780:798	the roc leader mRNA	780:798	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	0	15	from	factor	38:43	arg1	symbiont					64:71	a mammalian gut symbiont	48:71	a mammalian gut symbiont	48:71	Dietary sugar silences a colonization factor in a mammalian gut symbiont.
30559205	5	16	from	glucose	1059:1065	arg1	rich					1051:1054	rich	1051:1054	rich	1051:1054	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	2	17	theme	microbiota	256:265	arg1	composition					233:243	the composition	229:243	the composition of the gut microbiota	229:265	Dietary components are believed to influence the composition of the gut microbiota by serving as nutrients to a subset of microbes, thereby favoring their expansion.
30559205	6	18	theme	gut	1321:1323	arg1	colonization					1325:1336	gut colonization	1321:1336	gut colonization	1321:1336	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	4	19	theme	mRNA	795:798	arg1	Incorporation					763:775	Incorporation	763:775	Incorporation of the roc leader mRNA in front of a heterologous gene	763:830	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	3	20	from	diet	443:446	arg1	prevalent					418:426	prevalent	418:426	prevalent	418:426	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	1	21	theme	host	173:176	arg1	diet					178:181	the host diet	169:181	the host diet	169:181	The composition of the gut microbiota is largely determined by environmental factors including the host diet.
30559205	0	22	theme	Dietary	0:6	arg1	sugar					8:12	Dietary sugar	0:12	Dietary sugar	0:12	Dietary sugar silences a colonization factor in a mammalian gut symbiont.
30559205	3	23	theme	mRNA	688:691	arg1	region					674:679	the 5' leader region	660:679	the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization	660:760	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	4	24	theme	leader	788:793	arg1	mRNA					795:798	the roc leader mRNA	780:798	the roc leader mRNA	780:798	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	5	25	theme	B.	1012:1013	arg1	thetaiotaomicron					1015:1030	wild-type B. thetaiotaomicron	1002:1030	wild-type B. thetaiotaomicron	1002:1030	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	26	theme	engineered	928:937	arg1	strain					939:944	An engineered strain	925:944	An engineered strain refractory to Roc silencing by these sugars	925:988	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	4	27	dep	expression	884:893	arg1	off					880:882	off	880:882	off	880:882	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	6	28	theme	colonization	1325:1336	arg1	regulation					1307:1316	the microbiota: regulation	1291:1316	the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota	1291:1414	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	3	29	theme	commensal	578:586	arg1	Silencing					617:625	commensal Bacteroides thetaiotaomicron Silencing	578:625	commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose	578:649	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	3	30	theme	dietary	378:384	arg1	fructose					386:393	dietary fructose	378:393	dietary fructose	378:393	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	3	31	theme	Bacteroides	588:598	arg1	Silencing					617:625	commensal Bacteroides thetaiotaomicron Silencing	578:625	commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose	578:649	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	5	32	theme	refractory	946:955	arg1	strain					939:944	An engineered strain	925:944	An engineered strain refractory to Roc silencing by these sugars	925:988	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	0	33	theme	colonization	25:36	arg1	factor					38:43	a colonization factor	23:43	a colonization factor in a mammalian gut symbiont	23:71	Dietary sugar silences a colonization factor in a mammalian gut symbiont.
30559205	3	34	theme	human	568:572	arg1	gut					574:576	the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization	564:760	the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization	564:760	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	5	35	from	rich	1051:1054	arg1	mice					1141:1144	mice	1141:1144	mice fed a complex polysaccharide-rich diet	1141:1183	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	35	from	rich	1051:1054	arg1	glucose					1059:1065	glucose	1059:1065	glucose	1059:1065	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	36	from	mice	1141:1144	arg1	rich					1051:1054	rich	1051:1054	rich	1051:1054	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	5	37	theme	Roc	960:962	arg1	silencing					964:972	Roc silencing	960:972	Roc silencing by these sugars	960:988	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
30559205	3	38	from	prevalent	418:426	arg1	diet					443:446	the Western diet	431:446	the Western diet	431:446	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	1	39	theme	gut	97:99	arg1	microbiota					101:110	the gut microbiota	93:110	the gut microbiota	93:110	The composition of the gut microbiota is largely determined by environmental factors including the host diet.
30559205	4	40	theme	heterologous	814:825	arg1	gene					827:830	a heterologous gene	812:830	a heterologous gene	812:830	Incorporation of the roc leader mRNA in front of a heterologous gene was sufficient for fructose and glucose to turn off expression of the corresponding protein.
30559205	1	41	theme	microbiota	101:110	arg1	composition					78:88	The composition	74:88	The composition of the gut microbiota	74:110	The composition of the gut microbiota is largely determined by environmental factors including the host diet.
30559205	2	42	theme	gut	252:254	arg1	microbiota					256:265	the gut microbiota	248:265	the gut microbiota	248:265	Dietary components are believed to influence the composition of the gut microbiota by serving as nutrients to a subset of microbes, thereby favoring their expansion.
30559205	2	43	theme	microbes	306:313	arg1	subset					296:301	a subset	294:301	a subset of microbes	294:313	Dietary components are believed to influence the composition of the gut microbiota by serving as nutrients to a subset of microbes, thereby favoring their expansion.
30559205	3	44	theme	thetaiotaomicron	600:615	arg1	Silencing					617:625	commensal Bacteroides thetaiotaomicron Silencing	578:625	commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose	578:649	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	6	45	theme	microbiota	1295:1304	arg1	regulation					1307:1316	the microbiota: regulation	1291:1316	the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota	1291:1414	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	3	46	theme	Western	435:441	arg1	diet					443:446	the Western diet	431:446	the Western diet	431:446	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	0	47	theme	gut	60:62	arg1	symbiont					64:71	a mammalian gut symbiont	48:71	a mammalian gut symbiont	48:71	Dietary sugar silences a colonization factor in a mammalian gut symbiont.
30559205	3	48	theme	sugars	553:558	arg1	utilization					532:542	utilization	532:542	utilization of these sugars	532:558	However, we now report that dietary fructose and glucose, which are prevalent in the Western diet, specifically silence a protein that is necessary for gut colonization, but not for utilization of these sugars, by the human gut commensal Bacteroides thetaiotaomicron Silencing by fructose and glucose requires the 5' leader region of the mRNA specifying the protein, designated Roc for regulator of colonization.
30559205	6	49	theme	dietary	1221:1227	arg1	sugars					1229:1234	dietary sugars	1221:1234	dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota	1221:1414	Our findings underscore a role for dietary sugars that escape absorption by the host intestine and reach the microbiota: regulation of gut colonization by beneficial microbes independently of supplying nutrients to the microbiota.
30559205	0	50	theme	mammalian	50:58	arg1	symbiont					64:71	a mammalian gut symbiont	48:71	a mammalian gut symbiont	48:71	Dietary sugar silences a colonization factor in a mammalian gut symbiont.
30559205	5	51	theme	rich	1051:1054	arg1	diet					1046:1049	a diet	1044:1049	a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet	1044:1183	An engineered strain refractory to Roc silencing by these sugars outcompeted wild-type B. thetaiotaomicron in mice fed a diet rich in glucose and sucrose (a disaccharide composed of glucose and fructose), but not in mice fed a complex polysaccharide-rich diet.
31726797	5	0	theme	Caco-2	726:731	arg1	cells					733:737	H2O2-injured Caco-2 cells	713:737	H2O2-injured Caco-2 cells	713:737	Moreover, LW-XG exhibited excellent protective effect on H2O2-injured Caco-2 cells.
31726797	2	1	theme	molar	355:359	arg1	ratio					361:365	a molar ratio	353:365	a molar ratio of 1.63:1.5:1.0	353:381	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	2	2	from	glucose	312:318	arg1	ratio					361:365	a molar ratio	353:365	a molar ratio of 1.63:1.5:1.0	353:381	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	4	3	theme	reducing	640:647	arg1	power					649:653	good ferric reducing power	628:653	good ferric reducing power	628:653	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	6	4	theme	oxidative	852:860	arg1	damage					862:867	the oxidative damage	848:867	the oxidative damage induced by the overproduction of reactive oxygen species	848:924	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	2	5	from	mannose	321:327	arg1	ratio					361:365	a molar ratio	353:365	a molar ratio of 1.63:1.5:1.0	353:381	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	4	6	theme	scavenging	546:555	arg1	ability					557:563	a good scavenging ability	539:563	a good scavenging ability	539:563	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	5	7	theme	excellent	682:690	arg1	effect					703:708	excellent protective effect	682:708	excellent protective effect	682:708	Moreover, LW-XG exhibited excellent protective effect on H2O2-injured Caco-2 cells.
31726797	2	8	theme	LW-XG	302:306	arg1	composition					287:297	The monosaccharide composition	268:297	The monosaccharide composition of LW-XG	268:306	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	2	8	theme	LW-XG	302:306	arg1	glucose					312:318	glucose	312:318	glucose	312:318	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	4	9	theme	good	541:544	arg1	ability					557:563	a good scavenging ability	539:563	a good scavenging ability	539:563	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	5	10	theme	protective	692:701	arg1	effect					703:708	excellent protective effect	682:708	excellent protective effect	682:708	Moreover, LW-XG exhibited excellent protective effect on H2O2-injured Caco-2 cells.
31726797	3	11	theme	commercial	462:471	arg1	Da					493:494	2.95 × 106 Da	482:494	2.95 × 106 Da	482:494	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	3	11	theme	commercial	462:471	arg1	xanthan					473:479	commercial xanthan	462:479	commercial xanthan (2.95 × 106 Da)	462:495	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	2	12	from	acid	345:348	arg1	ratio					361:365	a molar ratio	353:365	a molar ratio of 1.63:1.5:1.0	353:381	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	4	13	theme	hydroxyl	606:613	arg1	radicals					615:622	hydroxyl radicals	606:622	hydroxyl radicals	606:622	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	1	14	theme	present	88:94	arg1	work					96:99	the present work	84:99	the present work	84:99	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	3	15	theme	molecular	388:396	arg1	Da					429:430	4.07 × 104 Da	418:430	4.07 × 104 Da	418:430	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	3	15	theme	molecular	388:396	arg1	weight					398:403	The molecular weight	384:403	The molecular weight of LW-XG	384:412	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	2	16	theme	1.63:1.5:1.0	370:381	arg1	ratio					361:365	a molar ratio	353:365	a molar ratio of 1.63:1.5:1.0	353:381	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	6	17	used	used	791:794	arg2	LW-XG					776:780	LW-XG	776:780	LW-XG	776:780	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	6	18	theme	work	756:759	arg1	Results					740:746	Results	740:746	Results of this work	740:759	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	1	19	theme	commercial	193:202	arg1	xanthan					204:210	commercial xanthan	193:210	commercial xanthan	193:210	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	0	20	theme	Antioxidant	21:31	arg1	Activity					33:40	Antioxidant Activity	21:40	Antioxidant Activity	21:40	Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum.
31726797	6	21	theme	species	918:924	arg1	overproduction					884:897	the overproduction	880:897	the overproduction of reactive oxygen species	880:924	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	1	22	theme	xanthan	204:210	arg1	biodegradation					175:188	biodegradation	175:188	biodegradation of commercial xanthan	175:210	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	3	23	theme	LW-XG	408:412	arg1	Da					429:430	4.07 × 104 Da	418:430	4.07 × 104 Da	418:430	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	3	23	theme	LW-XG	408:412	arg1	weight					398:403	The molecular weight	384:403	The molecular weight of LW-XG	384:412	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	6	24	theme	oxygen	911:916	arg1	species					918:924	reactive oxygen species	902:924	reactive oxygen species	902:924	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	3	25	theme	×	423:423	arg1	Da					429:430	4.07 × 104 Da	418:430	4.07 × 104 Da	418:430	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	3	25	theme	×	423:423	arg1	weight					398:403	The molecular weight	384:403	The molecular weight of LW-XG	384:412	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	4	26	theme	ferric	633:638	arg1	power					649:653	good ferric reducing power	628:653	good ferric reducing power	628:653	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	6	27	theme	reactive	902:909	arg1	species					918:924	reactive oxygen species	902:924	reactive oxygen species	902:924	Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.
31726797	0	28	theme	Xanthan	68:74	arg1	Gum					76:78	a Low-Molecular-Weight Xanthan Gum	45:78	a Low-Molecular-Weight Xanthan Gum	45:78	Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum.
31726797	1	29	dep	fungus	230:235	arg1	Chaetomiumglobosum					237:254	Chaetomiumglobosum	237:254	Chaetomiumglobosum	237:254	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	4	30	contain	had	535:537	arg2	ability					557:563	a good scavenging ability	539:563	a good scavenging ability	539:563	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	4	30	contain	had	535:537	arg1	LW-XG					529:533	LW-XG	529:533	LW-XG	529:533	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	1	31	theme	low-molecular-weight	104:123	arg1	gum					133:135	a low-molecular-weight xanthan gum	102:135	a low-molecular-weight xanthan gum (LW-XG)	102:143	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	1	31	theme	low-molecular-weight	104:123	arg1	LW-XG					138:142	LW-XG	138:142	LW-XG	138:142	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	0	32	theme	Low-Molecular-Weight	47:66	arg1	Gum					76:78	a Low-Molecular-Weight Xanthan Gum	45:78	a Low-Molecular-Weight Xanthan Gum	45:78	Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum.
31726797	4	33	theme	Antioxidant	498:508	arg1	assays					510:515	Antioxidant assays	498:515	Antioxidant assays	498:515	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	1	34	theme	xanthan	125:131	arg1	gum					133:135	a low-molecular-weight xanthan gum	102:135	a low-molecular-weight xanthan gum (LW-XG)	102:143	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	1	34	theme	xanthan	125:131	arg1	LW-XG					138:142	LW-XG	138:142	LW-XG	138:142	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	2	35	theme	monosaccharide	272:285	arg1	composition					287:297	The monosaccharide composition	268:297	The monosaccharide composition of LW-XG	268:306	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	2	35	theme	monosaccharide	272:285	arg1	glucose					312:318	glucose	312:318	glucose	312:318	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	5	36	theme	H2O2-injured	713:724	arg1	cells					733:737	H2O2-injured Caco-2 cells	713:737	H2O2-injured Caco-2 cells	713:737	Moreover, LW-XG exhibited excellent protective effect on H2O2-injured Caco-2 cells.
31726797	2	37	theme	glucuronic	334:343	arg1	acid					345:348	glucuronic acid	334:348	glucuronic acid	334:348	The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0.
31726797	1	38	theme	endophytic	219:228	arg1	6882					262:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	1	39	theme	fungus	230:235	arg1	6882					262:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
31726797	4	40	theme	superoxide	583:592	arg1	anions					594:599	superoxide anions	583:599	superoxide anions	583:599	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	3	41	theme	×	487:487	arg1	Da					493:494	2.95 × 106 Da	482:494	2.95 × 106 Da	482:494	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	3	41	theme	×	487:487	arg1	xanthan					473:479	commercial xanthan	462:479	commercial xanthan (2.95 × 106 Da)	462:495	The molecular weight of LW-XG was 4.07 × 104 Da and much smaller than that of commercial xanthan (2.95 × 106 Da).
31726797	0	42	theme	Gum	76:78	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum.
31726797	0	42	theme	Gum	76:78	arg1	Activity					33:40	Antioxidant Activity	21:40	Antioxidant Activity	21:40	Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum.
31726797	4	43	theme	DPPH	568:571	arg1	radicals					573:580	DPPH radicals	568:580	DPPH radicals	568:580	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	4	44	theme	good	628:631	arg1	power					649:653	good ferric reducing power	628:653	good ferric reducing power	628:653	Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power.
31726797	1	45	theme	CGMCC	256:260	arg1	6882					262:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	the endophytic fungus Chaetomiumglobosum CGMCC 6882	215:265	In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882.
30882809	0	0	theme	phosvitin	88:96	arg1	properties					70:79	physicochemical and emulsifying properties	38:79	physicochemical and emulsifying properties of egg phosvitin	38:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	7	1	theme	smaller	1039:1045	arg1	droplets					1051:1058	smaller oil droplets	1039:1058	smaller oil droplets	1039:1058	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	0	2	theme	egg	84:86	arg1	phosvitin					88:96	egg phosvitin	84:96	egg phosvitin	84:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	8	3	dep	ratio	1220:1224	arg1	 6					1232:1233	 6	1232:1233	a mass ratio of 1 : 6	1213:1233	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	3	4	theme	Polysaccharide	374:387	arg1	conjugation					389:399	Polysaccharide conjugation	374:399	Polysaccharide conjugation	374:399	Polysaccharide conjugation could improve the biofunctional properties of proteins via altering their structures.
30882809	0	5	from	Role	0:3	arg1	properties					70:79	physicochemical and emulsifying properties	38:79	physicochemical and emulsifying properties of egg phosvitin	38:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	8	6	theme	phosphate	1102:1110	arg1	groups					1112:1117	phosphate groups	1102:1117	phosphate groups	1102:1117	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	6	7	theme	surface	848:854	arg1	hydrophobicity					856:869	a lower surface hydrophobicity	840:869	a lower surface hydrophobicity	840:869	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	7	8	theme	tertiary	923:930	arg1	changes					943:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	8	9	theme	mass	1215:1218	arg1	ratio					1220:1224	a mass ratio	1213:1224	a mass ratio of 1 : 6	1213:1233	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	5	10	theme	more	757:760	arg1	groups					774:779	more hydrophobic groups	757:779	more hydrophobic groups	757:779	The results showed that PSV underwent an unfolding process when conjugated with pectin at a mass ratio of 1 : 2, exposing more hydrophobic groups.
30882809	6	11	theme	lower	842:846	arg1	hydrophobicity					856:869	a lower surface hydrophobicity	840:869	a lower surface hydrophobicity	840:869	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	4	12	with	conjugation	526:536	arg1	pectin					543:548	pectin	543:548	pectin	543:548	In this research, PSV was subjected to conjugation with pectin, and changes in physicochemical characteristics and functionalities were determined.
30882809	0	13	theme	binding	114:120	arg1	capacity					122:129	the calcium binding capacity	102:129	the calcium binding capacity of its phosphopeptides	102:152	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	8	14	attach	derived	1128:1134	arg2	PPP					1124:1126	the PPP	1120:1126	the PPP derived from copolymers	1120:1150	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	8	14	attach	derived	1128:1134	arg1	copolymers					1141:1150	copolymers	1141:1150	copolymers	1141:1150	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	7	15	theme	secondary	909:917	arg1	changes					943:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	0	16	theme	calcium	106:112	arg1	capacity					122:129	the calcium binding capacity	102:129	the calcium binding capacity of its phosphopeptides	102:152	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	0	17	theme	physicochemical	38:52	arg1	properties					70:79	physicochemical and emulsifying properties	38:79	physicochemical and emulsifying properties of egg phosvitin	38:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	2	18	theme	native	306:311	arg1	PSV					324:326	PSV	324:326	PSV	324:326	It is difficult to hydrolyze native phosvitin (PSV) to release PPP due to its compact structure.
30882809	2	18	theme	native	306:311	arg1	phosvitin					313:321	native phosvitin	306:321	native phosvitin (PSV)	306:327	It is difficult to hydrolyze native phosvitin (PSV) to release PPP due to its compact structure.
30882809	5	19	theme	hydrophobic	762:772	arg1	groups					774:779	more hydrophobic groups	757:779	more hydrophobic groups	757:779	The results showed that PSV underwent an unfolding process when conjugated with pectin at a mass ratio of 1 : 2, exposing more hydrophobic groups.
30882809	8	20	theme	functional	1277:1286	arg1	foods					1288:1292	functional foods	1277:1292	functional foods	1277:1292	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	3	21	theme	biofunctional	419:431	arg1	properties					433:442	the biofunctional properties	415:442	the biofunctional properties of proteins	415:454	Polysaccharide conjugation could improve the biofunctional properties of proteins via altering their structures.
30882809	6	22	theme	refolded	816:823	arg1	structure					825:833	a refolded structure	814:833	a refolded structure with a lower surface hydrophobicity and a higher thermal stability	814:900	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	0	23	theme	conjugation	23:33	arg1	Role					0:3	Role	0:3	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin	0:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	0	23	theme	conjugation	23:33	arg1	capacity					122:129	the calcium binding capacity	102:129	the calcium binding capacity of its phosphopeptides	102:152	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	5	24	theme	1 	741:742	arg1	ratio					732:736	a mass ratio	725:736	a mass ratio of 1 	725:742	The results showed that PSV underwent an unfolding process when conjugated with pectin at a mass ratio of 1 : 2, exposing more hydrophobic groups.
30882809	7	25	theme	PSV	990:992	arg1	properties					976:985	the emulsifying properties	960:985	the emulsifying properties of PSV	960:992	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	1	26	theme	calcium	208:214	arg1	bioavailability					216:230	calcium bioavailability	208:230	calcium bioavailability	208:230	Phosvitin phosphopeptides (PPP) effectively enhanced calcium bioavailability via inhibiting calcium-phosphate deposition.
30882809	0	27	theme	polysaccharide	8:21	arg1	conjugation					23:33	polysaccharide conjugation	8:33	polysaccharide conjugation	8:33	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	3	28	theme	proteins	447:454	arg1	properties					433:442	the biofunctional properties	415:442	the biofunctional properties of proteins	415:454	Polysaccharide conjugation could improve the biofunctional properties of proteins via altering their structures.
30882809	0	29	theme	phosphopeptides	138:152	arg1	Role					0:3	Role	0:3	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin	0:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	0	29	theme	phosphopeptides	138:152	arg1	capacity					122:129	the calcium binding capacity	102:129	the calcium binding capacity of its phosphopeptides	102:152	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	0	30	theme	emulsifying	58:68	arg1	properties					70:79	physicochemical and emulsifying properties	38:79	physicochemical and emulsifying properties of egg phosvitin	38:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30882809	7	31	with	production	1010:1019	arg1	droplets					1051:1058	smaller oil droplets	1039:1058	smaller oil droplets	1039:1058	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	8	32	theme	stronger	1164:1171	arg1	capacity					1189:1196	a stronger calcium binding capacity	1162:1196	a stronger calcium binding capacity	1162:1196	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	2	33	theme	compact	355:361	arg1	structure					363:371	its compact structure	351:371	its compact structure	351:371	It is difficult to hydrolyze native phosvitin (PSV) to release PPP due to its compact structure.
30882809	6	34	with	structure	825:833	arg1	stability					892:900	a higher thermal stability	875:900	a higher thermal stability	875:900	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	6	34	with	structure	825:833	arg1	hydrophobicity					856:869	a lower surface hydrophobicity	840:869	a lower surface hydrophobicity	840:869	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	4	35	theme	physicochemical	566:580	arg1	characteristics					582:596	physicochemical characteristics	566:596	physicochemical characteristics	566:596	In this research, PSV was subjected to conjugation with pectin, and changes in physicochemical characteristics and functionalities were determined.
30882809	5	36	theme	mass	727:730	arg1	ratio					732:736	a mass ratio	725:736	a mass ratio of 1 	725:742	The results showed that PSV underwent an unfolding process when conjugated with pectin at a mass ratio of 1 : 2, exposing more hydrophobic groups.
30882809	6	37	theme	thermal	884:890	arg1	stability					892:900	a higher thermal stability	875:900	a higher thermal stability	875:900	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	5	38	theme	unfolding	676:684	arg1	process					686:692	an unfolding process	673:692	an unfolding process	673:692	The results showed that PSV underwent an unfolding process when conjugated with pectin at a mass ratio of 1 : 2, exposing more hydrophobic groups.
30882809	6	39	theme	Excessive	782:790	arg1	glycosylation					792:804	Excessive glycosylation	782:804	Excessive glycosylation	782:804	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	8	40	theme	groups	1112:1117	arg1	redistribution					1084:1097	redistribution	1084:1097	redistribution of phosphate groups	1084:1117	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	7	41	theme	emulsifying	964:974	arg1	properties					976:985	the emulsifying properties	960:985	the emulsifying properties of PSV	960:992	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	8	42	theme	binding	1181:1187	arg1	capacity					1189:1196	a stronger calcium binding capacity	1162:1196	a stronger calcium binding capacity	1162:1196	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	6	43	theme	higher	877:882	arg1	stability					892:900	a higher thermal stability	875:900	a higher thermal stability	875:900	Excessive glycosylation induced a refolded structure with a lower surface hydrophobicity and a higher thermal stability.
30882809	7	44	theme	emulsions	1024:1032	arg1	production					1010:1019	the production	1006:1019	the production of emulsions with smaller oil droplets	1006:1058	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	1	45	theme	calcium-phosphate	247:263	arg1	deposition					265:274	calcium-phosphate deposition	247:274	calcium-phosphate deposition	247:274	Phosvitin phosphopeptides (PPP) effectively enhanced calcium bioavailability via inhibiting calcium-phosphate deposition.
30882809	8	46	theme	calcium	1173:1179	arg1	capacity					1189:1196	a stronger calcium binding capacity	1162:1196	a stronger calcium binding capacity	1162:1196	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	8	47	theme	1 	1229:1230	arg1	ratio					1220:1224	a mass ratio	1213:1224	a mass ratio of 1 : 6	1213:1233	Simultaneously, due to redistribution of phosphate groups, the PPP derived from copolymers exhibited a stronger calcium binding capacity, especially at a mass ratio of 1 : 6, possessing a potential to be utilized in functional foods.
30882809	7	48	theme	oil	1047:1049	arg1	droplets					1051:1058	smaller oil droplets	1039:1058	smaller oil droplets	1039:1058	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	2	49	theme	due	344:346	arg1	PPP					340:342	PPP	340:342	PPP due to its compact structure	340:371	It is difficult to hydrolyze native phosvitin (PSV) to release PPP due to its compact structure.
30882809	4	50	from	changes	555:561	arg1	functionalities					602:616	functionalities	602:616	functionalities	602:616	In this research, PSV was subjected to conjugation with pectin, and changes in physicochemical characteristics and functionalities were determined.
30882809	4	50	from	changes	555:561	arg1	characteristics					582:596	physicochemical characteristics	566:596	physicochemical characteristics	566:596	In this research, PSV was subjected to conjugation with pectin, and changes in physicochemical characteristics and functionalities were determined.
30882809	1	51	theme	Phosvitin	155:163	arg1	PPP					182:184	PPP	182:184	PPP	182:184	Phosvitin phosphopeptides (PPP) effectively enhanced calcium bioavailability via inhibiting calcium-phosphate deposition.
30882809	1	51	theme	Phosvitin	155:163	arg1	phosphopeptides					165:179	Phosvitin phosphopeptides	155:179	Phosvitin phosphopeptides (PPP)	155:185	Phosvitin phosphopeptides (PPP) effectively enhanced calcium bioavailability via inhibiting calcium-phosphate deposition.
30882809	7	52	theme	structural	932:941	arg1	changes					943:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes	903:949	These secondary and tertiary structural changes improved the emulsifying properties of PSV and allowed the production of emulsions with smaller oil droplets.
30882809	0	53	from	capacity	122:129	arg1	properties					70:79	physicochemical and emulsifying properties	38:79	physicochemical and emulsifying properties of egg phosvitin	38:96	Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides.
30452991	5	0	theme	3-ethylbenzothiazoline-6-sulfonic	813:845	arg1	2,2'-azino-bis					798:811	2,2'-azino-bis	798:811	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	798:858	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	0	theme	3-ethylbenzothiazoline-6-sulfonic	813:845	arg1	acid					847:850	3-ethylbenzothiazoline-6-sulfonic acid	813:850	3-ethylbenzothiazoline-6-sulfonic acid	813:850	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	2	1	from	effect	490:495	arg1	cells					522:526	H2O2-challenged IEC-6 cells	500:526	H2O2-challenged IEC-6 cells	500:526	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	2	2	theme	IEC-6	402:406	arg1	migration					413:421	IEC-6 cell migration	402:421	IEC-6 cell migration	402:421	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	0	3	theme	protective	96:105	arg1	effect					107:112	their protective effect	90:112	their protective effect against H2O2-induced oxidative damage in IEC-6 cells	90:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	0	4	from	characterization	12:27	arg1	hongkongensis					72:84	Crassostrea hongkongensis	60:84	Crassostrea hongkongensis	60:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	7	5	theme	survival	1175:1182	arg1	rate					1184:1187	the survival rate	1171:1187	the survival rate of H2O2-treated IEC-6 cells	1171:1215	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	1	6	from	activity	186:193	arg1	cells					245:249	intestine epithelial cells	224:249	intestine epithelial cells (IEC-6)	224:257	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	1	6	from	activity	186:193	arg1	IEC-6					252:256	IEC-6	252:256	IEC-6	252:256	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	0	7	theme	H2O2-induced	122:133	arg1	damage					145:150	H2O2-induced oxidative damage	122:150	H2O2-induced oxidative damage in IEC-6 cells	122:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	9	8	theme	NF-κB	1664:1668	arg1	pathway					1670:1676	the NF-κB pathway	1660:1676	the NF-κB pathway	1660:1676	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	9	9	theme	IEC-6	1567:1571	arg1	cells					1573:1577	IEC-6 cells	1567:1577	IEC-6 cells	1567:1577	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	9	10	theme	proinflammatory	1614:1628	arg1	secretion					1638:1646	proinflammatory factors secretion	1614:1646	proinflammatory factors secretion	1614:1646	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	5	11	theme	scavenging	768:777	arg1	activities					779:788	high scavenging activities	763:788	high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC)	763:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	0	12	from	hongkongensis	72:84	arg1	effect					107:112	their protective effect	90:112	their protective effect against H2O2-induced oxidative damage in IEC-6 cells	90:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	0	12	from	hongkongensis	72:84	arg1	characterization					12:27	Composition characterization	0:27	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis	0:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	0	12	from	hongkongensis	72:84	arg1	polysaccharides					39:53	oyster polysaccharides	32:53	oyster polysaccharides from Crassostrea hongkongensis	32:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	3	13	theme	IEC-6	604:608	arg1	migration					615:623	IEC-6 cell migration	604:623	IEC-6 cell migration	604:623	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	6	14	with	heteropolysaccharide	958:977	arg1	esters					993:998	sulfuric esters	984:998	sulfuric esters	984:998	And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa.
30452991	7	15	theme	intracellular	1230:1242	arg1	levels					1274:1279	intracellular reactive oxygen species (ROS) levels	1230:1279	intracellular reactive oxygen species (ROS) levels	1230:1279	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	9	16	theme	cell	1698:1701	arg1	apoptosis					1703:1711	cell apoptosis	1698:1711	cell apoptosis	1698:1711	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	6	17	theme	molecular	1083:1091	arg1	weight					1093:1098	an average molecular weight	1072:1098	an average molecular weight of 41.81 kDa	1072:1111	And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa.
30452991	3	18	theme	rapid	583:587	arg1	stimulation					589:599	rapid stimulation	583:599	rapid stimulation of IEC-6 cell migration	583:623	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	6	19	theme	41.81 kDa	1103:1111	arg1	weight					1093:1098	an average molecular weight	1072:1098	an average molecular weight of 41.81 kDa	1072:1111	And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa.
30452991	1	20	theme	oyster	198:203	arg1	polysaccharides					205:219	oyster polysaccharides	198:219	oyster polysaccharides	198:219	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	0	21	theme	oxidative	135:143	arg1	damage					145:150	H2O2-induced oxidative damage	122:150	H2O2-induced oxidative damage in IEC-6 cells	122:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	7	22	theme	H2O2-treated	1192:1203	arg1	cells					1211:1215	H2O2-treated IEC-6 cells	1192:1215	H2O2-treated IEC-6 cells	1192:1215	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	2	23	theme	H2O2-challenged	500:514	arg1	cells					522:526	H2O2-challenged IEC-6 cells	500:526	H2O2-challenged IEC-6 cells	500:526	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	1	24	theme	polysaccharides	205:219	arg1	activity					186:193	The proliferative activity	168:193	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6)	168:257	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	2	25	theme	IEC-6	516:520	arg1	cells					522:526	H2O2-challenged IEC-6 cells	500:526	H2O2-challenged IEC-6 cells	500:526	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	9	26	theme	radical	1583:1589	arg1	scavenging					1591:1600	radical scavenging	1583:1600	radical scavenging	1583:1600	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	2	27	theme	antioxidant	427:437	arg1	activity					439:446	antioxidant activity	427:446	antioxidant activity	427:446	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	0	28	theme	Composition	0:10	arg1	characterization					12:27	Composition characterization	0:27	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis	0:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	5	29	contain	possessed	753:761	arg1	fraction					744:751	The CHP2-3 fraction	733:751	The CHP2-3 fraction	733:751	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	29	contain	possessed	753:761	arg2	activities					779:788	high scavenging activities	763:788	high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC)	763:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	1	30	theme	intestine	224:232	arg1	cells					245:249	intestine epithelial cells	224:249	intestine epithelial cells (IEC-6)	224:257	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	1	30	theme	intestine	224:232	arg1	IEC-6					252:256	IEC-6	252:256	IEC-6	252:256	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	0	31	theme	oyster	32:37	arg1	polysaccharides					39:53	oyster polysaccharides	32:53	oyster polysaccharides from Crassostrea hongkongensis	32:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	5	32	theme	oxygen	864:869	arg1	ORAC					900:903	ORAC	900:903	ORAC	900:903	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	32	theme	oxygen	864:869	arg1	capacity					890:897	oxygen radical absorbance capacity	864:897	oxygen radical absorbance capacity (ORAC)	864:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	0	33	theme	IEC-6	155:159	arg1	cells					161:165	IEC-6 cells	155:165	IEC-6 cells	155:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	8	34	from	increases	1342:1350	arg1	secretion					1359:1367	the secretion	1355:1367	the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6)	1355:1418	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	3	35	theme	cell	610:613	arg1	migration					615:623	IEC-6 cell migration	604:623	IEC-6 cell migration	604:623	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	8	36	theme	nuclear	1436:1442	arg1	activation					1466:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	1	37	theme	epithelial	234:243	arg1	cells					245:249	intestine epithelial cells	224:249	intestine epithelial cells (IEC-6)	224:257	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	1	37	theme	epithelial	234:243	arg1	IEC-6					252:256	IEC-6	252:256	IEC-6	252:256	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	5	38	theme	absorbance	879:888	arg1	ORAC					900:903	ORAC	900:903	ORAC	900:903	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	38	theme	absorbance	879:888	arg1	capacity					890:897	oxygen radical absorbance capacity	864:897	oxygen radical absorbance capacity (ORAC)	864:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	39	theme	CHP2-3	737:742	arg1	fraction					744:751	The CHP2-3 fraction	733:751	The CHP2-3 fraction	733:751	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	0	40	from	effect	107:112	arg1	hongkongensis					72:84	Crassostrea hongkongensis	60:84	Crassostrea hongkongensis	60:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	3	41	theme	migration	615:623	arg1	stimulation					589:599	rapid stimulation	583:599	rapid stimulation of IEC-6 cell migration	583:623	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	3	42	theme	C30-60	533:538	arg1	%					539:539	%	539:539	%	539:539	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	7	43	theme	oxygen	1253:1258	arg1	ROS					1269:1271	ROS	1269:1271	ROS	1269:1271	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	7	43	theme	oxygen	1253:1258	arg1	species					1260:1266	reactive oxygen species	1244:1266	intracellular reactive oxygen species (ROS) levels	1230:1279	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	3	44	theme	polysaccharides	553:567	arg1	fraction					541:548	The C30-60% fraction	529:548	The C30-60% fraction of polysaccharides (CHP2)	529:574	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	9	45	theme	vital	1524:1528	arg1	role					1530:1533	a vital role	1522:1533	a vital role	1522:1533	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	8	46	theme	H2O2-mediated	1328:1340	arg1	increases					1342:1350	H2O2-mediated increases	1328:1350	H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6)	1328:1418	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	8	47	theme	interleukin-1β	1372:1385	arg1	secretion					1359:1367	the secretion	1355:1367	the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6)	1355:1418	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	6	48	theme	average	1075:1081	arg1	weight					1093:1098	an average molecular weight	1072:1098	an average molecular weight of 41.81 kDa	1072:1111	And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa.
30452991	0	49	theme	polysaccharides	39:53	arg1	effect					107:112	their protective effect	90:112	their protective effect against H2O2-induced oxidative damage in IEC-6 cells	90:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	0	49	theme	polysaccharides	39:53	arg1	characterization					12:27	Composition characterization	0:27	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis	0:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	7	50	theme	IEC-6	1205:1209	arg1	cells					1211:1215	H2O2-treated IEC-6 cells	1192:1215	H2O2-treated IEC-6 cells	1192:1215	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	5	51	theme	radical	871:877	arg1	ORAC					900:903	ORAC	900:903	ORAC	900:903	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	51	theme	radical	871:877	arg1	capacity					890:897	oxygen radical absorbance capacity	864:897	oxygen radical absorbance capacity (ORAC)	864:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	7	52	theme	cells	1211:1215	arg1	rate					1184:1187	the survival rate	1171:1187	the survival rate of H2O2-treated IEC-6 cells	1171:1215	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	5	53	theme	high	763:766	arg1	activities					779:788	high scavenging activities	763:788	high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC)	763:904	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	5	54	with	comparison	910:919	arg1	fractions					932:940	other fractions	926:940	other fractions	926:940	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	0	55	theme	Crassostrea	60:70	arg1	hongkongensis					72:84	Crassostrea hongkongensis	60:84	Crassostrea hongkongensis	60:84	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	7	56	theme	reactive	1244:1251	arg1	ROS					1269:1271	ROS	1269:1271	ROS	1269:1271	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	7	56	theme	reactive	1244:1251	arg1	species					1260:1266	reactive oxygen species	1244:1266	intracellular reactive oxygen species (ROS) levels	1230:1279	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	0	57	from	damage	145:150	arg1	cells					161:165	IEC-6 cells	155:165	IEC-6 cells	155:165	Composition characterization of oyster polysaccharides from Crassostrea hongkongensis and their protective effect against H2O2-induced oxidative damage in IEC-6 cells.
30452991	8	58	theme	factor-κB	1444:1452	arg1	activation					1466:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	7	59	theme	species	1260:1266	arg1	levels					1274:1279	intracellular reactive oxygen species (ROS) levels	1230:1279	intracellular reactive oxygen species (ROS) levels	1230:1279	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	2	60	theme	polysaccharides	375:389	arg1	ability					357:363	the ability	353:363	the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells	353:526	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	6	61	theme	sulfuric	984:991	arg1	esters					993:998	sulfuric esters	984:998	sulfuric esters	984:998	And CHP2-3 was heteropolysaccharide with sulfuric esters, and it was mainly composed of glucose, galactose and arabinose and had an average molecular weight of 41.81 kDa.
30452991	9	62	theme	oxidative	1547:1555	arg1	damage					1557:1562	oxidative damage	1547:1562	oxidative damage in IEC-6 cells	1547:1577	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	2	63	theme	oyster	368:373	arg1	polysaccharides					375:389	oyster polysaccharides	368:389	oyster polysaccharides	368:389	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	8	64	theme	interleukin-6	1399:1411	arg1	secretion					1359:1367	the secretion	1355:1367	the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6)	1355:1418	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	8	65	theme	NF-κB	1455:1459	arg1	activation					1466:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	9	66	theme	factors	1630:1636	arg1	secretion					1638:1646	proinflammatory factors secretion	1614:1646	proinflammatory factors secretion	1614:1646	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	1	67	theme	proliferative	172:184	arg1	activity					186:193	The proliferative activity	168:193	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6)	168:257	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	3	68	theme	%	539:539	arg1	fraction					541:548	The C30-60% fraction	529:548	The C30-60% fraction of polysaccharides (CHP2)	529:574	The C30-60% fraction of polysaccharides (CHP2) showed rapid stimulation of IEC-6 cell migration after wounding.
30452991	7	69	with	Pretreatment	1114:1125	arg1	CHP2-3					1141:1146	CHP2-3	1141:1146	CHP2-3	1141:1146	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	7	69	with	Pretreatment	1114:1125	arg1	CHP2					1132:1135	CHP2	1132:1135	CHP2	1132:1135	Pretreatment with CHP2 and CHP2-3 significantly improved the survival rate of H2O2-treated IEC-6 cells, and reduced intracellular reactive oxygen species (ROS) levels.
30452991	2	70	theme	cytoprotective	475:488	arg1	effect					490:495	their cytoprotective effect	469:495	their cytoprotective effect on H2O2-challenged IEC-6 cells	469:526	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	5	71	theme	other	926:930	arg1	fractions					932:940	other fractions	926:940	other fractions	926:940	The CHP2-3 fraction possessed high scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC), in comparison with other fractions.
30452991	1	72	theme	5-fluorouracil-induced	270:291	arg1	inflammation					304:315	5-fluorouracil-induced intestinal inflammation	270:315	5-fluorouracil-induced intestinal inflammation	270:315	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
30452991	9	73	from	damage	1557:1562	arg1	cells					1573:1577	IEC-6 cells	1567:1577	IEC-6 cells	1567:1577	These results indicate that CHP2-3 may play a vital role in reducing oxidative damage in IEC-6 cells via radical scavenging, decreasing proinflammatory factors secretion, inhibiting the NF-κB pathway, and thus, reducing cell apoptosis.
30452991	8	74	theme	p65	1462:1464	arg1	activation					1466:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	nuclear factor-κB (NF-κB) p65 activation	1436:1475	Moreover, CHP2-3 also significantly decreased H2O2-mediated increases in the secretion of interleukin-1β (IL-1β) and interleukin-6 (IL-6), and attenuated nuclear factor-κB (NF-κB) p65 activation.
30452991	2	75	theme	cell	408:411	arg1	migration					413:421	IEC-6 cell migration	402:421	IEC-6 cell migration	402:421	In this study, we aimed to measure the ability of oyster polysaccharides to promote IEC-6 cell migration and antioxidant activity and further describe their cytoprotective effect on H2O2-challenged IEC-6 cells.
30452991	1	76	theme	intestinal	293:302	arg1	inflammation					304:315	5-fluorouracil-induced intestinal inflammation	270:315	5-fluorouracil-induced intestinal inflammation	270:315	The proliferative activity of oyster polysaccharides in intestine epithelial cells (IEC-6) alleviated 5-fluorouracil-induced intestinal inflammation.
29897509	4	0	theme	serum	940:944	arg1	IgG					946:948	serum IgG	940:948	serum IgG	940:948	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	6	1	dep	performance	1179:1189	arg1	the					1168:1170	the	1168:1170	the	1168:1170	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	4	2	theme	digestive	836:844	arg1	protease					876:883	protease	876:883	protease	876:883	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	2	theme	digestive	836:844	arg1	peroxidase					924:933	glutathione peroxidase	912:933	glutathione peroxidase	912:933	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	2	theme	digestive	836:844	arg1	amylase					855:861	amylase	855:861	amylase	855:861	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	2	theme	digestive	836:844	arg1	lipase					864:869	lipase	864:869	lipase	864:869	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	2	theme	digestive	836:844	arg1	dismutase					898:906	superoxide dismutase	887:906	superoxide dismutase	887:906	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	2	theme	digestive	836:844	arg1	enzymes					846:852	digestive enzymes	836:852	digestive enzymes (amylase, lipase, and protease)	836:884	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	5	3	theme	AMP	1041:1043	arg1	dose					1045:1048	excessive AMP dose	1031:1048	excessive AMP dose (>1 g kg-1)	1031:1060	However, excessive AMP dose (>1 g kg-1) could not improve its efficiency further.
29897509	5	3	theme	AMP	1041:1043	arg1	kg-1					1056:1059	>1 g kg-1	1051:1059	>1 g kg-1	1051:1059	However, excessive AMP dose (>1 g kg-1) could not improve its efficiency further.
29897509	1	4	from	effects	154:160	arg1	immunity					264:271	immunity	264:271	immunity	264:271	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	1	4	from	effects	154:160	arg1	performance					248:258	growth performance	241:258	growth performance	241:258	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	0	5	theme	juvenile	99:106	arg1	broilers					108:115	juvenile broilers	99:115	juvenile broilers	99:115	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	0	6	from	Effect	0:5	arg1	immunity					87:94	immunity	87:94	immunity	87:94	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	0	6	from	Effect	0:5	arg1	performance					71:81	growth performance	64:81	growth performance	64:81	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	6	7	theme	immune	1195:1200	arg1	responses					1202:1210	immune responses	1195:1210	immune responses	1195:1210	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	2	8	theme	liquid	312:317	arg1	chromatography					319:332	High-performance liquid chromatography	295:332	High-performance liquid chromatography	295:332	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	4	9	theme	malondialdehyde	982:996	arg1	level					998:1002	lower malondialdehyde level	976:1002	lower malondialdehyde level	976:1002	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	10	theme	superoxide	887:896	arg1	enzymes					846:852	digestive enzymes	836:852	digestive enzymes (amylase, lipase, and protease)	836:884	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	10	theme	superoxide	887:896	arg1	dismutase					898:906	superoxide dismutase	887:906	superoxide dismutase	887:906	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	IgM					951:953	IgM	951:953	IgM	951:953	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	gains					811:815	higher body weight gains	792:815	higher body weight gains	792:815	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	activities					822:831	the activities	818:831	the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase	818:933	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	level					998:1002	lower malondialdehyde level	976:1002	lower malondialdehyde level	976:1002	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	IgA					960:962	IgA	960:962	IgA	960:962	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	11	theme	enzymes	846:852	arg1	IgG					946:948	serum IgG	940:948	serum IgG	940:948	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	2	12	theme	High-performance	295:310	arg1	chromatography					319:332	High-performance liquid chromatography	295:332	High-performance liquid chromatography	295:332	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	1	13	theme	juvenile	276:283	arg1	broilers					285:292	juvenile broilers	276:292	juvenile broilers	276:292	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	4	14	theme	glutathione	912:922	arg1	enzymes					846:852	digestive enzymes	836:852	digestive enzymes (amylase, lipase, and protease)	836:884	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	14	theme	glutathione	912:922	arg1	peroxidase					924:933	glutathione peroxidase	912:933	glutathione peroxidase	912:933	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	1	15	theme	dietary	165:171	arg1	supplementation					218:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	0	16	theme	broilers	108:115	arg1	immunity					87:94	immunity	87:94	immunity	87:94	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	0	16	theme	broilers	108:115	arg1	performance					71:81	growth performance	64:81	growth performance	64:81	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	3	17	theme	juvenile	688:695	arg1	broilers					697:704	juvenile broilers	688:704	juvenile broilers	688:704	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	6	18	theme	growth	1172:1177	arg1	performance					1179:1189	growth performance	1172:1189	growth performance	1172:1189	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	4	19	theme	juvenile	734:741	arg1	broilers					743:750	the juvenile broilers	730:750	the juvenile broilers fed with AMP-containing diets	730:780	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	1	20	theme	Astragalus	173:182	arg1	supplementation					218:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	3	21	theme	broilers	697:704	arg1	immunity					676:683	immunity	676:683	immunity	676:683	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	3	21	theme	broilers	697:704	arg1	performance					660:670	the growth performance	649:670	the growth performance	649:670	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	4	22	dep	gains	811:815	arg1	levels					964:969	levels	964:969	levels	964:969	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	2	23	theme	sugar	347:351	arg1	analysis					365:372	High-performance liquid chromatography spectrum and sugar composition analysis	295:372	analysis	365:372	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	1	24	theme	membranaceus	184:195	arg1	supplementation					218:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	1	25	theme	broilers	285:292	arg1	immunity					264:271	immunity	264:271	immunity	264:271	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	1	25	theme	broilers	285:292	arg1	performance					248:258	growth performance	241:258	growth performance	241:258	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	1	26	theme	polysaccharide	197:210	arg1	supplementation					218:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	0	27	theme	Astragalus	18:27	arg1	polysaccharide					42:55	dietary Astragalus membranaceus polysaccharide	10:55	dietary Astragalus membranaceus polysaccharide	10:55	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	0	28	dep	performance	71:81	arg1	the					60:62	the	60:62	the	60:62	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	0	29	theme	dietary	10:16	arg1	polysaccharide					42:55	dietary Astragalus membranaceus polysaccharide	10:55	dietary Astragalus membranaceus polysaccharide	10:55	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	2	30	theme	chromatography	319:332	arg1	spectrum					334:341	High-performance liquid chromatography spectrum and sugar composition analysis	295:372	spectrum	334:341	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	1	31	theme	AMP	213:215	arg1	supplementation					218:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	dietary Astragalus membranaceus polysaccharide (AMP) supplementation	165:232	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	0	32	theme	polysaccharide	42:55	arg1	Effect					0:5	Effect	0:5	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.	0:116	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	1	33	dep	performance	248:258	arg1	the					237:239	the	237:239	the	237:239	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	4	34	theme	higher	792:797	arg1	gains					811:815	higher body weight gains	792:815	higher body weight gains	792:815	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	2	35	theme	11,078 Da	446:454	arg1	weight					436:441	a molecular weight	424:441	a molecular weight of 11,078 Da	424:454	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	0	36	theme	membranaceus	29:40	arg1	polysaccharide					42:55	dietary Astragalus membranaceus polysaccharide	10:55	dietary Astragalus membranaceus polysaccharide	10:55	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	4	37	dep	enzymes	846:852	arg1	lipase					864:869	lipase	864:869	lipase	864:869	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	37	dep	enzymes	846:852	arg1	protease					876:883	protease	876:883	protease	876:883	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	37	dep	enzymes	846:852	arg1	amylase					855:861	amylase	855:861	amylase	855:861	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	4	37	dep	enzymes	846:852	arg1	enzymes					846:852	digestive enzymes	836:852	digestive enzymes (amylase, lipase, and protease)	836:884	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	6	38	theme	AMP	1152:1154	arg1	administration					1134:1147	dietary administration	1126:1147	dietary administration of AMP	1126:1154	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	1	39	theme	supplementation	218:232	arg1	effects					154:160	the effects	150:160	the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers	150:292	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	4	40	theme	weight	804:809	arg1	gains					811:815	higher body weight gains	792:815	higher body weight gains	792:815	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	5	41	theme	excessive	1031:1039	arg1	dose					1045:1048	excessive AMP dose	1031:1048	excessive AMP dose (>1 g kg-1)	1031:1060	However, excessive AMP dose (>1 g kg-1) could not improve its efficiency further.
29897509	5	41	theme	excessive	1031:1039	arg1	kg-1					1056:1059	>1 g kg-1	1051:1059	>1 g kg-1	1051:1059	However, excessive AMP dose (>1 g kg-1) could not improve its efficiency further.
29897509	4	42	theme	body	799:802	arg1	gains					811:815	higher body weight gains	792:815	higher body weight gains	792:815	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	6	43	theme	broilers	1224:1231	arg1	performance					1179:1189	growth performance	1172:1189	growth performance	1172:1189	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	6	43	theme	broilers	1224:1231	arg1	responses					1202:1210	immune responses	1195:1210	immune responses	1195:1210	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	3	44	theme	growth	653:658	arg1	performance					660:670	the growth performance	649:670	the growth performance	649:670	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	6	45	theme	dietary	1126:1132	arg1	administration					1134:1147	dietary administration	1126:1147	dietary administration of AMP	1126:1154	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	4	46	theme	feeding	721:727	arg1	wk					715:716	6 wk	713:716	6 wk of feeding	713:727	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	6	47	theme	juvenile	1215:1222	arg1	broilers					1224:1231	juvenile broilers	1215:1231	juvenile broilers	1215:1231	Results indicate that dietary administration of AMP can improve the growth performance and immune responses of juvenile broilers.
29897509	2	48	with	heteropolysaccharide	398:417	arg1	weight					436:441	a molecular weight	424:441	a molecular weight of 11,078 Da	424:454	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	3	49	theme	AMP	571:573	arg1	levels					561:566	four different levels	546:566	four different levels of AMP (0, 0.5, 1, and 2 g kg-1)	546:599	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	2	50	theme	composition	353:363	arg1	analysis					365:372	High-performance liquid chromatography spectrum and sugar composition analysis	295:372	analysis	365:372	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	3	51	from	effects	638:644	arg1	immunity					676:683	immunity	676:683	immunity	676:683	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	3	51	from	effects	638:644	arg1	performance					660:670	the growth performance	649:670	the growth performance	649:670	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	2	52	theme	molecular	426:434	arg1	weight					436:441	a molecular weight	424:441	a molecular weight of 11,078 Da	424:454	High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose.
29897509	1	53	theme	growth	241:246	arg1	performance					248:258	growth performance	241:258	growth performance	241:258	This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers.
29897509	0	54	theme	growth	64:69	arg1	performance					71:81	growth performance	64:81	growth performance	64:81	Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers.
29897509	4	55	theme	AMP-containing	761:774	arg1	diets					776:780	AMP-containing diets	761:780	AMP-containing diets	761:780	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
29897509	3	56	theme	different	551:559	arg1	levels					561:566	four different levels	546:566	four different levels of AMP (0, 0.5, 1, and 2 g kg-1)	546:599	Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers.
29897509	4	57	theme	lower	976:980	arg1	level					998:1002	lower malondialdehyde level	976:1002	lower malondialdehyde level	976:1002	After 6 wk of feeding, the juvenile broilers fed with AMP-containing diets exhibited higher body weight gains; the activities of digestive enzymes (amylase, lipase, and protease), superoxide dismutase and glutathione peroxidase; and serum IgG, IgM, and IgA levels; and lower malondialdehyde level than the control.
31226378	4	0	theme	air	448:450	arg1	permeability					452:463	air permeability	448:463	air permeability (porosity)	448:474	Films were characterized by SEM, air permeability (porosity), tensile strength, burst strength, TGA and WVP.
31226378	4	0	theme	air	448:450	arg1	porosity					466:473	porosity	466:473	porosity	466:473	Films were characterized by SEM, air permeability (porosity), tensile strength, burst strength, TGA and WVP.
31226378	8	1	dep	friendly	1082:1089	arg1	sustainable					1105:1115	sustainable	1105:1115	sustainable	1105:1115	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	8	1	dep	friendly	1082:1089	arg1	low-cost					1092:1099	low-cost	1092:1099	low-cost	1092:1099	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	8	2	theme	composite	1035:1043	arg1	materials					1045:1053	cellulosic composite materials	1024:1053	cellulosic composite materials	1024:1053	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	6	3	theme	aggregation	837:847	arg1	signs					818:822	signs	818:822	signs of particles aggregation	818:847	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	5	4	theme	Antimicrobial	524:536	arg1	activity					538:545	Antimicrobial activity	524:545	Antimicrobial activity in opposition to E. coli, B. mycoides and C. albicans	524:599	Antimicrobial activity in opposition to E. coli, B. mycoides and C. albicans were also investigated.
31226378	5	5	from	activity	538:545	arg1	opposition					550:559	opposition	550:559	opposition to E. coli, B. mycoides and C. albicans	550:599	Antimicrobial activity in opposition to E. coli, B. mycoides and C. albicans were also investigated.
31226378	6	6	dep	antimicrobial	688:700	arg1	reduce					771:776	reduce	771:776	reduce porosity & air permeability	771:804	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	7	theme	particles	827:835	arg1	aggregation					837:847	particles aggregation	827:847	particles aggregation	827:847	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	2	8	from	gelatin	303:309	arg1	compositions					277:288	the film compositions	268:288	the film compositions from CMC and gelatin or shellac	268:320	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	2	9	theme	film	272:275	arg1	compositions					277:288	the film compositions	268:288	the film compositions from CMC and gelatin or shellac	268:320	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	2	10	from	CMC	295:297	arg1	compositions					277:288	the film compositions	268:288	the film compositions from CMC and gelatin or shellac	268:320	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	8	11	theme	friendly	1082:1089	arg1	materials					1127:1135	environmentally friendly, low-cost and sustainable packaging materials	1066:1135	environmentally friendly, low-cost and sustainable packaging materials	1066:1135	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	2	12	from	shellac	314:320	arg1	compositions					277:288	the film compositions	268:288	the film compositions from CMC and gelatin or shellac	268:320	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	1	13	theme	carboxymethyl	158:170	arg1	CMC					183:185	CMC	183:185	CMC	183:185	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	1	13	theme	carboxymethyl	158:170	arg1	cellulose					172:180	carboxymethyl cellulose	158:180	carboxymethyl cellulose (CMC)	158:186	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	3	14	theme	composite	327:335	arg1	films					337:341	The composite films	323:341	The composite films	323:341	The composite films were prepared by solution casting in presence of glycerol (30% weight).
31226378	7	15	theme	%	868:868	arg1	one					899:901	one	899:901	one	899:901	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	7	15	theme	%	868:868	arg1	film					871:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	0	16	theme	natural	6:12	arg1	films					24:28	Novel natural composite films	0:28	Novel natural composite films as packaging materials with enhanced properties	0:76	Novel natural composite films as packaging materials with enhanced properties.
31226378	3	17	from	casting	369:375	arg1	presence					380:387	presence	380:387	presence of glycerol (30% weight)	380:412	The composite films were prepared by solution casting in presence of glycerol (30% weight).
31226378	1	18	theme	present	83:89	arg1	work					91:94	The present work	79:94	The present work	79:94	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	0	19	theme	Novel	0:4	arg1	films					24:28	Novel natural composite films	0:28	Novel natural composite films as packaging materials with enhanced properties	0:76	Novel natural composite films as packaging materials with enhanced properties.
31226378	4	20	theme	burst	495:499	arg1	strength					501:508	burst strength	495:508	burst strength	495:508	Films were characterized by SEM, air permeability (porosity), tensile strength, burst strength, TGA and WVP.
31226378	6	21	theme	tensile	703:709	arg1	strength					711:718	tensile strength	703:718	tensile strength	703:718	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	21	theme	tensile	703:709	arg1	antimicrobial					688:700	good antimicrobial	683:700	good antimicrobial	683:700	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	7	22	theme	50/50	863:867	arg1	one					899:901	one	899:901	one	899:901	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	7	22	theme	50/50	863:867	arg1	film					871:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	0	23	theme	composite	14:22	arg1	films					24:28	Novel natural composite films	0:28	Novel natural composite films as packaging materials with enhanced properties	0:76	Novel natural composite films as packaging materials with enhanced properties.
31226378	0	24	theme	packaging	33:41	arg1	materials					43:51	packaging materials	33:51	packaging materials	33:51	Novel natural composite films as packaging materials with enhanced properties.
31226378	7	25	theme	thermal	959:965	arg1	stability					967:975	a high thermal stability	952:975	a high thermal stability up to 800 °C	952:988	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	6	26	theme	air	789:791	arg1	permeability					793:804	porosity & air permeability	778:804	porosity & air permeability	778:804	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	2	27	theme	composites	218:227	arg1	series					235:240	Four composites films series	213:240	Four composites films series	213:240	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	3	28	theme	%	404:404	arg1	weight					406:411	30% weight	402:411	30% weight	402:411	The composite films were prepared by solution casting in presence of glycerol (30% weight).
31226378	3	28	theme	%	404:404	arg1	glycerol					392:399	glycerol	392:399	glycerol (30% weight)	392:412	The composite films were prepared by solution casting in presence of glycerol (30% weight).
31226378	3	29	theme	glycerol	392:399	arg1	presence					380:387	presence	380:387	presence of glycerol (30% weight)	380:412	The composite films were prepared by solution casting in presence of glycerol (30% weight).
31226378	6	30	theme	&	787:787	arg1	permeability					793:804	porosity & air permeability	778:804	porosity & air permeability	778:804	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	0	31	with	films	24:28	arg1	properties					67:76	enhanced properties	58:76	enhanced properties	58:76	Novel natural composite films as packaging materials with enhanced properties.
31226378	8	32	theme	packaging	1117:1125	arg1	materials					1127:1135	environmentally friendly, low-cost and sustainable packaging materials	1066:1135	environmentally friendly, low-cost and sustainable packaging materials	1066:1135	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	6	33	theme	porosity	778:785	arg1	permeability					793:804	porosity & air permeability	778:804	porosity & air permeability	778:804	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	34	theme	good	683:686	arg1	strength					711:718	tensile strength	703:718	tensile strength	703:718	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	34	theme	good	683:686	arg1	transparency					734:745	transparency	734:745	transparency	734:745	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	34	theme	good	683:686	arg1	properties					759:768	elongation properties	748:768	elongation properties	748:768	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	34	theme	good	683:686	arg1	homogeneity					721:731	homogeneity	721:731	homogeneity	721:731	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	34	theme	good	683:686	arg1	antimicrobial					688:700	good antimicrobial	683:700	good antimicrobial	683:700	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	7	35	theme	high	954:957	arg1	stability					967:975	a high thermal stability	952:975	a high thermal stability up to 800 °C	952:988	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	4	36	theme	tensile	477:483	arg1	strength					485:492	tensile strength	477:492	tensile strength	477:492	Films were characterized by SEM, air permeability (porosity), tensile strength, burst strength, TGA and WVP.
31226378	7	37	theme	thermal	919:925	arg1	stability					927:935	thermal stability	919:935	thermal stability	919:935	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	1	38	theme	novel	119:123	arg1	films					143:147	novel natural composite films	119:147	novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac	119:210	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	7	39	theme	Gelatin/CMC	850:860	arg1	one					899:901	one	899:901	one	899:901	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	7	39	theme	Gelatin/CMC	850:860	arg1	film					871:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film	850:874	Gelatin/CMC (50/50%) film was the most important one with respect to thermal stability, which exhibit a high thermal stability up to 800 °C.
31226378	1	40	theme	natural	125:131	arg1	films					143:147	novel natural composite films	119:147	novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac	119:210	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	8	41	theme	cellulosic	1024:1033	arg1	materials					1045:1053	cellulosic composite materials	1024:1053	cellulosic composite materials	1024:1053	This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials.
31226378	6	42	theme	elongation	748:757	arg1	properties					759:768	elongation properties	748:768	elongation properties	748:768	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	6	42	theme	elongation	748:757	arg1	antimicrobial					688:700	good antimicrobial	683:700	good antimicrobial	683:700	The results revealed that, films exhibit flexibility with good antimicrobial, tensile strength, homogeneity, transparency, elongation properties, reduce porosity & air permeability and without signs of particles aggregation.
31226378	1	43	theme	composite	133:141	arg1	films					143:147	novel natural composite films	119:147	novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac	119:210	The present work was devoted to prepare novel natural composite films based on carboxymethyl cellulose (CMC), gelatin and/or shellac.
31226378	0	44	theme	enhanced	58:65	arg1	properties					67:76	enhanced properties	58:76	enhanced properties	58:76	Novel natural composite films as packaging materials with enhanced properties.
31226378	2	45	theme	films	229:233	arg1	series					235:240	Four composites films series	213:240	Four composites films series	213:240	Four composites films series were prepared by altering the film compositions from CMC and gelatin or shellac.
31226378	3	46	theme	solution	360:367	arg1	casting					369:375	solution casting	360:375	solution casting in presence of glycerol (30% weight)	360:412	The composite films were prepared by solution casting in presence of glycerol (30% weight).
29408578	0	0	theme	cell	104:107	arg1	viability					109:117	In vitro cell viability	95:117	In vitro cell viability	95:117	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	5	1	theme	serum	799:803	arg1	albumin					805:811	bovine serum albumin	792:811	bovine serum albumin	792:811	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	7	2	theme	human	1132:1136	arg1	hMSCs					1162:1166	hMSCs	1162:1166	hMSCs	1162:1166	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	7	2	theme	human	1132:1136	arg1	cells					1155:1159	the human mesenchymal stem cells	1128:1159	the human mesenchymal stem cells (hMSCs)	1128:1167	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	5	3	theme	prepared	851:858	arg1	scaffolds					860:868	all the prepared scaffolds	843:868	all the prepared scaffolds	843:868	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	6	4	theme	higher	919:924	arg1	concentration					929:941	the higher HA concentration	915:941	the higher HA concentration (40wt%)	915:949	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	4	theme	higher	919:924	arg1	%					948:948	40wt%	944:948	40wt%	944:948	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	5	5	theme	albumin	805:811	arg1	adsorption					778:787	adsorption	778:787	adsorption	778:787	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	5	5	theme	albumin	805:811	arg1	SBF					770:772	SBF	770:772	SBF	770:772	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	5	5	theme	albumin	805:811	arg1	viability					829:837	cell viability	824:837	SBF and adsorption of bovine serum albumin as well as cell viability	770:837	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	1	6	dep	in	286:287	arg1	vitro					289:293	vitro	289:293	vitro	289:293	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	7	7	theme	mesenchymal	1138:1148	arg1	hMSCs					1162:1166	hMSCs	1162:1166	hMSCs	1162:1166	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	7	7	theme	mesenchymal	1138:1148	arg1	cells					1155:1159	the human mesenchymal stem cells	1128:1159	the human mesenchymal stem cells (hMSCs)	1128:1167	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	2	8	theme	lyophilization	366:379	arg1	technique					381:389	the lyophilization technique	362:389	the lyophilization technique	362:389	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	5	9	from	bioactivity	755:765	arg1	adsorption					778:787	adsorption	778:787	adsorption	778:787	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	5	9	from	bioactivity	755:765	arg1	SBF					770:772	SBF	770:772	SBF	770:772	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	5	9	from	bioactivity	755:765	arg1	viability					829:837	cell viability	824:837	SBF and adsorption of bovine serum albumin as well as cell viability	770:837	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	9	10	used	used	1422:1425	arg2	material					1404:1411	This novel composite material	1383:1411	This novel composite material	1383:1411	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	10	used	used	1422:1425	arg2	scaffold					1459:1466	a scaffold material to deliver cells	1457:1492	a scaffold material to deliver cells	1457:1492	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	10	used	used	1422:1425	arg2	molecules					1518:1526	biologically active molecules	1498:1526	biologically active molecules	1498:1526	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	6	11	theme	HA	926:927	arg1	concentration					929:941	the higher HA concentration	915:941	the higher HA concentration (40wt%)	915:949	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	11	theme	HA	926:927	arg1	%					948:948	40wt%	944:948	40wt%	944:948	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	9	12	theme	tissue	1435:1440	arg1	engineering					1442:1452	bone tissue engineering	1430:1452	bone tissue engineering	1430:1452	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	5	13	theme	in	746:747	arg1	bioactivity					755:765	The in vitro bioactivity	742:765	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds	742:868	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	1	14	theme	enhanced	277:284	arg1	regeneration					300:311	enhanced in vitro bone regeneration	277:311	enhanced in vitro bone regeneration	277:311	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	7	15	theme	fabricated	1106:1115	arg1	systems					1117:1123	the fabricated systems	1102:1123	the fabricated systems	1102:1123	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	3	16	theme	scaffolds	565:573	arg1	properties					538:547	The physicochemical, thermal behavior and morphology properties	485:547	properties	538:547	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	5	17	theme	cell	824:827	arg1	viability					829:837	cell viability	824:837	SBF and adsorption of bovine serum albumin as well as cell viability	770:837	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	9	18	theme	bone	1430:1433	arg1	engineering					1442:1452	bone tissue engineering	1430:1452	bone tissue engineering	1430:1452	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	7	19	theme	systems	1117:1123	arg1	non-toxicity					1086:1097	the non-toxicity	1082:1097	the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs)	1082:1167	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	2	20	theme	hydrogel	466:473	arg1	networks					475:482	composite hydrogel networks	456:482	composite hydrogel networks	456:482	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	7	21	from	non-toxicity	1086:1097	arg1	hMSCs					1162:1166	hMSCs	1162:1166	hMSCs	1162:1166	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	7	21	from	non-toxicity	1086:1097	arg1	cells					1155:1159	the human mesenchymal stem cells	1128:1159	the human mesenchymal stem cells (hMSCs)	1128:1167	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	4	22	theme	investigated	680:691	arg1	scaffolds					693:701	investigated scaffolds	680:701	investigated scaffolds	680:701	Furthermore, the mechanical behavior of the under investigated scaffolds was determined using texture analyzer.
29408578	0	23	theme	alginate/hydroxyethyl	6:26	arg1	scaffold					63:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold	0:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.	0:168	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	5	24	dep	in	746:747	arg1	vitro					749:753	vitro	749:753	vitro	749:753	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	1	25	theme	/hydroxyethylcellulose	190:211	arg1	HA					235:236	HA	235:236	HA	235:236	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	1	25	theme	/hydroxyethylcellulose	190:211	arg1	/hydroxyapatite					218:232	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite	170:232	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds	170:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	0	26	theme	Novel	0:4	arg1	scaffold					63:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold	0:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.	0:168	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	1	27	theme	in	286:287	arg1	regeneration					300:311	enhanced in vitro bone regeneration	277:311	enhanced in vitro bone regeneration	277:311	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	2	28	theme	SA/HEC/HA	318:326	arg1	composites					328:337	The SA/HEC/HA composites	314:337	The SA/HEC/HA composites	314:337	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	0	29	theme	composite	53:61	arg1	scaffold					63:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold	0:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.	0:168	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	8	30	theme	Proliferation	1170:1182	arg1	capability					1184:1193	Proliferation capability	1170:1193	Proliferation capability	1170:1193	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	0	31	theme	mesenchymal	146:156	arg1	cells					163:167	human mesenchymal stem cells	140:167	human mesenchymal stem cells	140:167	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	0	32	dep	In	95:96	arg1	vitro					98:102	vitro	98:102	vitro	98:102	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	3	33	theme	physicochemical	489:503	arg1	properties					538:547	The physicochemical, thermal behavior and morphology properties	485:547	properties	538:547	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	0	34	theme	cellulose/hydroxyapatite	28:51	arg1	scaffold					63:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold	0:70	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.	0:168	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	1	35	theme	bone	295:298	arg1	regeneration					300:311	enhanced in vitro bone regeneration	277:311	enhanced in vitro bone regeneration	277:311	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	0	36	theme	human	140:144	arg1	cells					163:167	human mesenchymal stem cells	140:167	human mesenchymal stem cells	140:167	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	2	37	theme	ions	443:446	arg1	presence					423:430	the presence	419:430	the presence of calcium ions	419:446	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	8	38	theme	tested	1222:1227	arg1	scaffolds					1229:1237	the tested scaffolds	1218:1237	the tested scaffolds after 3 and 7days	1218:1255	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	6	39	theme	mechanical	964:973	arg1	bioactivity					997:1007	bioactivity	997:1007	bioactivity	997:1007	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	39	theme	mechanical	964:973	arg1	properties					975:984	the mechanical properties	960:984	the mechanical properties (23.9MPa)	960:994	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	39	theme	mechanical	964:973	arg1	adsorption					1021:1030	protein adsorption	1013:1030	protein adsorption	1013:1030	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	39	theme	mechanical	964:973	arg1	23.9MPa					987:993	23.9MPa	987:993	23.9MPa	987:993	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	0	40	theme	cells	163:167	arg1	proliferation					123:135	proliferation	123:135	proliferation	123:135	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	0	40	theme	cells	163:167	arg1	viability					109:117	In vitro cell viability	95:117	In vitro cell viability	95:117	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	2	41	theme	calcium	435:441	arg1	ions					443:446	calcium ions	435:446	calcium ions	435:446	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	1	42	dep	/hydroxyethylcellulose	190:211	arg1	HEC					214:216	HEC	214:216	HEC	214:216	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	1	43	theme	/hydroxyapatite	218:232	arg1	scaffolds					249:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds	170:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds	170:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	0	44	theme	stem	158:161	arg1	cells					163:167	human mesenchymal stem cells	140:167	human mesenchymal stem cells	140:167	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	8	45	theme	HA-containing	1273:1285	arg1	scaffold					1287:1294	the higher HA-containing scaffold	1262:1294	the higher HA-containing scaffold	1262:1294	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	2	46	theme	composite	456:464	arg1	networks					475:482	composite hydrogel networks	456:482	composite hydrogel networks	456:482	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	4	47	theme	the	670:672	arg1	behavior					658:665	the mechanical behavior	643:665	the mechanical behavior of the under investigated scaffolds	643:701	Furthermore, the mechanical behavior of the under investigated scaffolds was determined using texture analyzer.
29408578	7	48	theme	stem	1150:1153	arg1	hMSCs					1162:1166	hMSCs	1162:1166	hMSCs	1162:1166	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	7	48	theme	stem	1150:1153	arg1	cells					1155:1159	the human mesenchymal stem cells	1128:1159	the human mesenchymal stem cells (hMSCs)	1128:1167	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	9	49	theme	active	1511:1516	arg1	molecules					1518:1526	biologically active molecules	1498:1526	biologically active molecules	1498:1526	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	49	theme	active	1511:1516	arg1	material					1404:1411	This novel composite material	1383:1411	This novel composite material	1383:1411	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	7	50	theme	Cell	1033:1036	arg1	viability					1038:1046	Cell viability	1033:1046	Cell viability of the tested scaffolds	1033:1070	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	0	51	theme	bone	76:79	arg1	regeneration					81:92	bone regeneration	76:92	bone regeneration	76:92	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	9	52	theme	novel	1388:1392	arg1	molecules					1518:1526	biologically active molecules	1498:1526	biologically active molecules	1498:1526	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	52	theme	novel	1388:1392	arg1	scaffold					1459:1466	a scaffold material to deliver cells	1457:1492	a scaffold material to deliver cells	1457:1492	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	52	theme	novel	1388:1392	arg1	material					1404:1411	This novel composite material	1383:1411	This novel composite material	1383:1411	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	7	53	theme	tested	1055:1060	arg1	scaffolds					1062:1070	the tested scaffolds	1051:1070	the tested scaffolds	1051:1070	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	0	54	dep	scaffold	63:70	arg1	proliferation					123:135	proliferation	123:135	proliferation	123:135	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	0	54	dep	scaffold	63:70	arg1	viability					109:117	In vitro cell viability	95:117	In vitro cell viability	95:117	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	3	55	theme	morphology	527:536	arg1	properties					538:547	The physicochemical, thermal behavior and morphology properties	485:547	properties	538:547	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	9	56	theme	composite	1394:1402	arg1	molecules					1518:1526	biologically active molecules	1498:1526	biologically active molecules	1498:1526	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	56	theme	composite	1394:1402	arg1	scaffold					1459:1466	a scaffold material to deliver cells	1457:1492	a scaffold material to deliver cells	1457:1492	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	9	56	theme	composite	1394:1402	arg1	material					1404:1411	This novel composite material	1383:1411	This novel composite material	1383:1411	This novel composite material could be used in bone tissue engineering as a scaffold material to deliver cells and biologically active molecules.
29408578	3	57	theme	thermal	506:512	arg1	properties					538:547	The physicochemical, thermal behavior and morphology properties	485:547	properties	538:547	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	8	58	theme	HA-free	1364:1370	arg1	scaffolds					1372:1380	HA-free scaffolds	1364:1380	HA-free scaffolds	1364:1380	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	2	59	attach	cross-linked	403:414	arg3	presence					423:430	the presence	419:430	the presence of calcium ions	419:446	The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks.
29408578	3	60	theme	behavior	514:521	arg1	properties					538:547	The physicochemical, thermal behavior and morphology properties	485:547	properties	538:547	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	8	61	theme	cell	1313:1316	arg1	populations					1318:1328	increased cell populations	1303:1328	increased cell populations	1303:1328	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	0	62	theme	In	95:96	arg1	viability					109:117	In vitro cell viability	95:117	In vitro cell viability	95:117	Novel alginate/hydroxyethyl cellulose/hydroxyapatite composite scaffold for bone regeneration: In vitro cell viability and proliferation of human mesenchymal stem cells.
29408578	8	63	theme	higher	1266:1271	arg1	scaffold					1287:1294	the higher HA-containing scaffold	1262:1294	the higher HA-containing scaffold	1262:1294	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	4	64	theme	mechanical	647:656	arg1	behavior					658:665	the mechanical behavior	643:665	the mechanical behavior of the under investigated scaffolds	643:701	Furthermore, the mechanical behavior of the under investigated scaffolds was determined using texture analyzer.
29408578	4	65	theme	texture	724:730	arg1	analyzer					732:739	texture analyzer	724:739	texture analyzer	724:739	Furthermore, the mechanical behavior of the under investigated scaffolds was determined using texture analyzer.
29408578	8	66	theme	increased	1303:1311	arg1	populations					1318:1328	increased cell populations	1303:1328	increased cell populations	1303:1328	Proliferation capability was also confirmed for the tested scaffolds after 3 and 7days, but the higher HA-containing scaffold showed increased cell populations specially after 7days compared to HA-free scaffolds.
29408578	1	67	theme	composite	239:247	arg1	scaffolds					249:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds	170:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds	170:257	Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration.
29408578	5	68	theme	bovine	792:797	arg1	albumin					805:811	bovine serum albumin	792:811	bovine serum albumin	792:811	The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
29408578	7	69	theme	scaffolds	1062:1070	arg1	viability					1038:1046	Cell viability	1033:1046	Cell viability of the tested scaffolds	1033:1070	Cell viability of the tested scaffolds confirmed the non-toxicity of the fabricated systems on the human mesenchymal stem cells (hMSCs).
29408578	3	70	theme	prepared	556:563	arg1	scaffolds					565:573	the prepared scaffolds	552:573	the prepared scaffolds	552:573	The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM.
29408578	6	71	theme	protein	1013:1019	arg1	adsorption					1021:1030	protein adsorption	1013:1030	protein adsorption	1013:1030	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29408578	6	71	theme	protein	1013:1019	arg1	properties					975:984	the mechanical properties	960:984	the mechanical properties (23.9MPa)	960:994	The results indicated that the higher HA concentration (40wt%) enhanced the mechanical properties (23.9MPa), bioactivity and protein adsorption.
29636094	8	0	theme	green	2237:2241	arg1	turtles					2243:2249	juvenile green turtles	2228:2249	juvenile green turtles after settlement into coastal habitats	2228:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	3	1	from	waters	677:682	arg1	turtles					652:658	24 young wild and captive green turtles	620:658	24 young wild and captive green turtles from the coastal waters of Brazil	620:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	1	from	waters	677:682	arg1	rectum					610:615	rectum	610:615	rectum	610:615	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	1	from	waters	677:682	arg1	feces					600:604	the feces	596:604	the feces	596:604	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	4	2	theme	different	1274:1282	arg1	units					1306:1310	6321 different operational taxonomic units	1269:1310	6321 different operational taxonomic units (at 97% sequence homology cutoff)	1269:1344	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	2	3	theme	Green	381:385	arg1	turtles					387:393	Green turtles	381:393	Green turtles (Chelonia mydas)	381:410	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	2	3	theme	Green	381:385	arg1	mydas					405:409	Chelonia mydas	396:409	Chelonia mydas	396:409	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	7	4	theme	tropical	1942:1949	arg1	regions					1973:1979	tropical than from subtropical regions	1942:1979	tropical than from subtropical regions	1942:1979	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	8	5	theme	general	2092:2098	arg1	microbiota					2100:2109	the same general microbiota	2083:2109	the same general microbiota	2083:2109	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	1	6	theme	herbivory	277:285	arg1	acquisition					262:272	the independent acquisition	246:272	the independent acquisition of herbivory	246:285	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	3	7	theme	curved	700:705	arg1	length					716:721	curved carapace length	700:721	curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome	700:917	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	7	8	theme	subtropical	1961:1971	arg1	regions					1973:1979	tropical than from subtropical regions	1942:1979	tropical than from subtropical regions	1942:1979	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	3	9	theme	gut	987:989	arg1	microbiome					991:1000	the gut microbiome	983:1000	the gut microbiome of green turtles from tropical and subtropical regions	983:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	4	10	theme	97	1316:1317	arg1	%					1318:1318	%	1318:1318	%	1318:1318	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	1	11	theme	cellulose	193:201	arg1	assimilation					221:232	cellulose and hemicellulose assimilation	193:232	assimilation	221:232	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	3	12	theme	green	1133:1137	arg1	turtles					1139:1145	green turtles	1133:1145	green turtles	1133:1145	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	4	13	theme	sequence	1320:1327	arg1	homology					1329:1336	97% sequence homology	1316:1336	97% sequence homology cutoff	1316:1343	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	8	14	dep	CONCLUSIONS	1982:1992	arg1	indicate					2008:2015	indicate	2008:2015	indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin	2008:2145	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	8	14	dep	CONCLUSIONS	1982:1992	arg1	suggest					2152:2158	suggest	2152:2158	suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats	2152:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	1	15	theme	hemicellulose	207:219	arg1	assimilation					221:232	cellulose and hemicellulose assimilation	193:232	assimilation	221:232	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	7	16	theme	turtle	1899:1904	arg1	size					1906:1909	turtle size	1899:1909	turtle size	1899:1909	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	4	17	theme	taxonomic	1296:1304	arg1	units					1306:1310	6321 different operational taxonomic units	1269:1310	6321 different operational taxonomic units (at 97% sequence homology cutoff)	1269:1344	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	3	18	dep	modifies	1102:1109	arg1	3					1063:1063	3	1063:1063	3	1063:1063	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	5	19	theme	high	1558:1561	arg1	levels					1563:1568	high levels	1558:1568	high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1558:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	8	20	contain	had	2079:2081	arg2	microbiota					2100:2109	the same general microbiota	2083:2109	the same general microbiota	2083:2109	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	8	20	contain	had	2079:2081	arg1	turtles					2037:2043	juvenile green turtles	2022:2043	juvenile green turtles from the coastal waters of Brazil	2022:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	3	21	from	change	857:862	arg1	diversity					887:895	diversity	887:895	diversity	887:895	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	21	from	change	857:862	arg1	composition					871:881	the composition	867:881	the composition	867:881	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	22	theme	wild	629:632	arg1	turtles					652:658	24 young wild and captive green turtles	620:658	24 young wild and captive green turtles from the coastal waters of Brazil	620:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	5	23	theme	Prophyromonas	1589:1601	arg1	Firmicutes					1543:1552	Firmicutes	1543:1552	Firmicutes	1543:1552	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	23	theme	Prophyromonas	1589:1601	arg1	levels					1563:1568	high levels	1558:1568	high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1558:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	3	24	dep	microbiota	574:583	arg1	modifies					1102:1109	modifies	1102:1109	modifies the gut microbiota of green turtles	1102:1145	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	24	dep	microbiota	574:583	arg1	exist					936:940	exist	936:940	exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions	936:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	5	25	theme	green	1395:1399	arg1	turtles					1401:1407	the juvenile green turtles	1382:1407	the juvenile green turtles less than 45 cm of curved carapace length	1382:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	6	26	theme	mixed	1838:1842	arg1	diet					1844:1847	a macroalgae/fish mixed diet	1820:1847	a macroalgae/fish mixed diet	1820:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	3	27	theme	ontogenetic	789:799	arg1	shift					809:813	the ontogenetic dietary shift	785:813	the ontogenetic dietary shift after settlement	785:830	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	0	28	theme	green	75:79	arg1	turtles					81:87	juvenile green turtles	66:87	juvenile green turtles	66:87	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	3	29	theme	turtles	1011:1017	arg1	microbiome					991:1000	the gut microbiome	983:1000	the gut microbiome of green turtles from tropical and subtropical regions	983:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	2	30	theme	pelagic	536:542	arg1	animals					544:550	pelagic animals	536:550	pelagic animals	536:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	5	31	theme	less	1409:1412	arg1	turtles					1401:1407	the juvenile green turtles	1382:1407	the juvenile green turtles less than 45 cm of curved carapace length	1382:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	4	32	dep	rRNA	1207:1210	arg1	reads					1212:1216	reads	1212:1216	reads	1212:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	3	33	theme	subtropical	1037:1047	arg1	regions					1049:1055	tropical and subtropical regions	1024:1055	tropical and subtropical regions	1024:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	34	dep	followed	835:842	arg1	1					782:782	1	782:782	1	782:782	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	0	35	theme	Fast	0:3	arg1	acquisition					5:15	Fast acquisition	0:15	Fast acquisition of a polysaccharide fermenting gut	0:50	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	3	36	theme	tropical	1024:1031	arg1	regions					1049:1055	tropical and subtropical regions	1024:1055	tropical and subtropical regions	1024:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	4	37	theme	rRNA	1207:1210	arg1	library					1166:1172	A genomic library	1156:1172	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads	1148:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	2	38	theme	marine	443:448	arg1	tetrapods					450:458	marine tetrapods	443:458	marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals	443:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	6	39	theme	green	1730:1734	arg1	CCL					1752:1754	wild green turtles > 45 cm CCL	1725:1754	wild green turtles > 45 cm CCL	1725:1754	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	5	40	theme	length	1444:1449	arg1	45 cm					1419:1423	45 cm	1419:1423	45 cm of curved carapace length	1419:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	3	41	from	feces	600:604	arg1	present					585:591	present	585:591	present	585:591	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	41	from	feces	600:604	arg1	waters					677:682	the coastal waters	665:682	the coastal waters of Brazil	665:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	2	42	theme	groups	433:438	arg1	tetrapods					450:458	marine tetrapods	443:458	marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals	443:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	2	42	theme	groups	433:438	arg1	one					416:418	one	416:418	one	416:418	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	2	42	theme	groups	433:438	arg1	groups					433:438	the three groups	423:438	the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals	423:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	3	43	theme	turtles	652:658	arg1	rectum					610:615	rectum	610:615	rectum	610:615	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	43	theme	turtles	652:658	arg1	feces					600:604	the feces	596:604	the feces	596:604	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	44	from	rectum	610:615	arg1	present					585:591	present	585:591	present	585:591	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	44	from	rectum	610:615	arg1	waters					677:682	the coastal waters	665:682	the coastal waters of Brazil	665:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	5	45	theme	Ruminococaceae	1604:1617	arg1	Firmicutes					1543:1552	Firmicutes	1543:1552	Firmicutes	1543:1552	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	45	theme	Ruminococaceae	1604:1617	arg1	levels					1563:1568	high levels	1558:1568	high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1558:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	0	46	theme	polysaccharide	22:35	arg1	gut					48:50	a polysaccharide fermenting gut	20:50	a polysaccharide fermenting gut	20:50	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	1	47	theme	new	317:319	arg1	relationships					335:347	new endosymbiotic relationships	317:347	new endosymbiotic relationships between tetrapods and microbes	317:378	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	8	48	theme	fermenting	2199:2208	arg1	microbiota					2214:2223	a polysaccharide fermenting gut microbiota	2182:2223	a polysaccharide fermenting gut microbiota	2182:2223	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	8	49	theme	green	2031:2035	arg1	turtles					2037:2043	juvenile green turtles	2022:2043	juvenile green turtles from the coastal waters of Brazil	2022:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	2	50	theme	herbivorous	468:478	arg1	diet					480:483	an herbivorous diet	465:483	an herbivorous diet and which acquire it after several years consuming pelagic animals	465:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	3	51	theme	captive	638:644	arg1	turtles					652:658	24 young wild and captive green turtles	620:658	24 young wild and captive green turtles from the coastal waters of Brazil	620:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	0	52	theme	gut	48:50	arg1	acquisition					5:15	Fast acquisition	0:15	Fast acquisition of a polysaccharide fermenting gut	0:50	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	1	53	theme	relationships	335:347	arg1	establishment					300:312	the establishment	296:312	the establishment of new endosymbiotic relationships between tetrapods and microbes	296:378	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	8	54	theme	microbiota	2214:2223	arg1	acquisition					2167:2177	a fast acquisition	2160:2177	a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats	2160:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	3	55	theme	gut	904:906	arg1	microbiome					908:917	the gut microbiome	900:917	the gut microbiome	900:917	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	6	56	theme	only	1692:1695	arg1	profile					1708:1714	the only microbiota profile	1688:1714	the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1688:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	56	theme	only	1692:1695	arg1	this					1679:1682	this	1679:1682	this	1679:1682	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	5	57	theme	curved	1428:1433	arg1	length					1444:1449	curved carapace length	1428:1449	curved carapace length	1428:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	7	58	from	regions	1973:1979	arg1	turtles					1929:1935	turtles	1929:1935	turtles from tropical than from subtropical regions	1929:1979	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	8	59	theme	coastal	2054:2060	arg1	waters					2062:2067	the coastal waters	2050:2067	the coastal waters of Brazil	2050:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	4	60	theme	bacterial	1193:1201	arg1	rRNA					1207:1210	2,186,596 valid bacterial 16S rRNA reads	1177:1216	2,186,596 valid bacterial 16S rRNA reads	1177:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	2	61	theme	Chelonia	396:403	arg1	turtles					387:393	Green turtles	381:393	Green turtles (Chelonia mydas)	381:410	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	2	61	theme	Chelonia	396:403	arg1	mydas					405:409	Chelonia mydas	396:409	Chelonia mydas	396:409	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	5	62	theme	phyla	1519:1523	arg1	representatives					1496:1510	representatives	1496:1510	representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1496:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	1	63	theme	independent	250:260	arg1	acquisition					262:272	the independent acquisition	246:272	the independent acquisition of herbivory	246:285	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	3	64	theme	omnivorous	1085:1094	arg1	diets					1096:1100	omnivorous diets	1085:1100	omnivorous diets	1085:1100	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	8	65	from	waters	2062:2067	arg1	turtles					2037:2043	juvenile green turtles	2022:2043	juvenile green turtles from the coastal waters of Brazil	2022:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	7	66	theme	microbial	1864:1872	arg1	diversity					1874:1882	microbial diversity	1864:1882	microbial diversity	1864:1882	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	3	67	theme	Brazil	687:692	arg1	waters					677:682	the coastal waters	665:682	the coastal waters of Brazil	665:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	8	68	theme	coastal	2273:2279	arg1	habitats					2281:2288	coastal habitats	2273:2288	coastal habitats	2273:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	8	69	theme	same	2087:2090	arg1	microbiota					2100:2109	the same general microbiota	2083:2109	the same general microbiota	2083:2109	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	6	70	theme	> 45 cm	1744:1750	arg1	CCL					1752:1754	wild green turtles > 45 cm CCL	1725:1754	wild green turtles > 45 cm CCL	1725:1754	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	4	71	from	cutoff	1338:1343	arg1	units					1306:1310	6321 different operational taxonomic units	1269:1310	6321 different operational taxonomic units (at 97% sequence homology cutoff)	1269:1344	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	3	72	theme	gut	1115:1117	arg1	microbiota					1119:1128	the gut microbiota	1111:1128	the gut microbiota of green turtles	1111:1145	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	73	theme	carapace	707:714	arg1	length					716:721	curved carapace length	700:721	curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome	700:917	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	74	theme	present	585:591	arg1	differences					924:934	present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences	585:934	present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences	585:934	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	75	theme	coastal	669:675	arg1	waters					677:682	the coastal waters	665:682	the coastal waters of Brazil	665:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	6	76	theme	size	1804:1807	arg1	turtles					1789:1795	the captive green turtles	1771:1795	the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1771:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	0	77	theme	coastal	124:130	arg1	habitats					132:139	coastal habitats	124:139	coastal habitats	124:139	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	3	78	theme	turtles	1139:1145	arg1	microbiota					1119:1128	the gut microbiota	1111:1128	the gut microbiota of green turtles	1111:1145	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	8	79	theme	body	2126:2129	arg1	size					2131:2134	body size	2126:2134	body size	2126:2134	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	4	80	theme	%	1318:1318	arg1	homology					1329:1336	97% sequence homology	1316:1336	97% sequence homology cutoff	1316:1343	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	3	81	with	turtles	652:658	arg1	length					716:721	curved carapace length	700:721	curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome	700:917	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	82	from	microbiome	991:1000	arg1	regions					1049:1055	tropical and subtropical regions	1024:1055	tropical and subtropical regions	1024:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	4	83	theme	homology	1329:1336	arg1	cutoff					1338:1343	97% sequence homology cutoff	1316:1343	97% sequence homology cutoff	1316:1343	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	6	84	located	found	1716:1720	arg1	turtles					1789:1795	the captive green turtles	1771:1795	the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1771:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	84	located	found	1716:1720	arg2	this					1679:1682	this	1679:1682	this	1679:1682	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	84	located	found	1716:1720	arg1	CCL					1752:1754	wild green turtles > 45 cm CCL	1725:1754	wild green turtles > 45 cm CCL	1725:1754	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	84	located	found	1716:1720	arg1	most					1763:1766	most	1763:1766	most	1763:1766	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	84	located	found	1716:1720	arg2	profile					1708:1714	the only microbiota profile	1688:1714	the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1688:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	85	theme	green	1783:1787	arg1	turtles					1789:1795	the captive green turtles	1771:1795	the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1771:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	4	86	theme	operational	1284:1294	arg1	units					1306:1310	6321 different operational taxonomic units	1269:1310	6321 different operational taxonomic units (at 97% sequence homology cutoff)	1269:1344	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	0	87	from	settlement	110:119	arg1	habitats					132:139	coastal habitats	124:139	coastal habitats	124:139	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	3	88	theme	young	623:627	arg1	turtles					652:658	24 young wild and captive green turtles	620:658	24 young wild and captive green turtles from the coastal waters of Brazil	620:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	2	89	with	tetrapods	450:458	arg1	diet					480:483	an herbivorous diet	465:483	an herbivorous diet and which acquire it after several years consuming pelagic animals	465:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	7	90	dep	than	1951:1954	arg1	from					1956:1959	from	1956:1959	from	1956:1959	Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions.
29636094	3	91	from	present	585:591	arg1	rectum					610:615	rectum	610:615	rectum	610:615	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	91	from	present	585:591	arg1	feces					600:604	the feces	596:604	the feces	596:604	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	0	92	theme	juvenile	66:73	arg1	turtles					81:87	juvenile green turtles	66:87	juvenile green turtles	66:87	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	1	93	theme	BACKGROUND	142:151	arg1	Tetrapods					153:161	BACKGROUND Tetrapods	142:161	BACKGROUND Tetrapods	142:161	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	8	94	theme	fast	2162:2165	arg1	acquisition					2167:2177	a fast acquisition	2160:2177	a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats	2160:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	3	95	theme	green	1005:1009	arg1	turtles					1011:1017	green turtles	1005:1017	green turtles from tropical and subtropical regions	1005:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	96	theme	gradual	849:855	arg1	change					857:862	a gradual change	847:862	a gradual change in the composition and diversity of the gut microbiome	847:917	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	97	from	regions	1049:1055	arg1	microbiome					991:1000	the gut microbiome	983:1000	the gut microbiome of green turtles from tropical and subtropical regions	983:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	97	from	regions	1049:1055	arg1	turtles					1011:1017	green turtles	1005:1017	green turtles from tropical and subtropical regions	1005:1055	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	8	98	theme	juvenile	2228:2235	arg1	turtles					2243:2249	juvenile green turtles	2228:2249	juvenile green turtles after settlement into coastal habitats	2228:2288	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	4	99	theme	genomic	1158:1164	arg1	library					1166:1172	A genomic library	1156:1172	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads	1148:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	6	100	theme	macroalgae/fish	1822:1836	arg1	diet					1844:1847	a macroalgae/fish mixed diet	1820:1847	a macroalgae/fish mixed diet	1820:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	3	101	theme	dietary	801:807	arg1	shift					809:813	the ontogenetic dietary shift	785:813	the ontogenetic dietary shift after settlement	785:830	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	102	theme	microbiome	991:1000	arg1	composition					954:964	the composition	950:964	the composition	950:964	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	102	theme	microbiome	991:1000	arg1	diversity					970:978	diversity	970:978	diversity	970:978	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	103	attach	present	585:591	arg1	rectum					610:615	rectum	610:615	rectum	610:615	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	103	attach	present	585:591	arg2	differences					924:934	present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences	585:934	present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences	585:934	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	103	attach	present	585:591	arg1	feces					600:604	the feces	596:604	the feces	596:604	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	2	104	theme	several	512:518	arg1	years					520:524	several years	512:524	several years consuming pelagic animals	512:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	5	105	theme	Lachnospiraceae	1624:1638	arg1	Firmicutes					1543:1552	Firmicutes	1543:1552	Firmicutes	1543:1552	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	105	theme	Lachnospiraceae	1624:1638	arg1	levels					1563:1568	high levels	1558:1568	high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1558:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	2	106	theme	tetrapods	450:458	arg1	tetrapods					450:458	marine tetrapods	443:458	marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals	443:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	2	106	theme	tetrapods	450:458	arg1	groups					433:438	the three groups	423:438	the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals	423:550	Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals.
29636094	6	107	theme	turtles	1736:1742	arg1	CCL					1752:1754	wild green turtles > 45 cm CCL	1725:1754	wild green turtles > 45 cm CCL	1725:1754	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	5	108	theme	juvenile	1386:1393	arg1	turtles					1401:1407	the juvenile green turtles	1382:1407	the juvenile green turtles less than 45 cm of curved carapace length	1382:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	109	dep	phyla	1519:1523	arg1	Firmicutes					1543:1552	Firmicutes	1543:1552	Firmicutes	1543:1552	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	109	dep	phyla	1519:1523	arg1	levels					1563:1568	high levels	1558:1568	high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1558:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	5	109	dep	phyla	1519:1523	arg1	Bacteroidetes					1525:1537	the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1515:1663	the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1515:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	8	110	theme	juvenile	2022:2029	arg1	turtles					2037:2043	juvenile green turtles	2022:2043	juvenile green turtles from the coastal waters of Brazil	2022:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	6	111	theme	wild	1725:1728	arg1	CCL					1752:1754	wild green turtles > 45 cm CCL	1725:1754	wild green turtles > 45 cm CCL	1725:1754	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	3	112	theme	green	646:650	arg1	turtles					652:658	24 young wild and captive green turtles	620:658	24 young wild and captive green turtles from the coastal waters of Brazil	620:692	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	5	113	theme	latter	1651:1656	arg1	phylum					1658:1663	the latter phylum	1647:1663	the latter phylum	1647:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	0	114	theme	fermenting	37:46	arg1	gut					48:50	a polysaccharide fermenting gut	20:50	a polysaccharide fermenting gut	20:50	Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
29636094	5	115	theme	fecal	1463:1467	arg1	microbiota					1469:1478	a fecal microbiota	1461:1478	a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum	1461:1663	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	1	116	theme	endosymbiotic	321:333	arg1	relationships					335:347	new endosymbiotic relationships	317:347	new endosymbiotic relationships between tetrapods and microbes	317:378	BACKGROUND Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes.
29636094	8	117	theme	polysaccharide	2184:2197	arg1	microbiota					2214:2223	a polysaccharide fermenting gut microbiota	2182:2223	a polysaccharide fermenting gut microbiota	2182:2223	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	6	118	theme	microbiota	1697:1706	arg1	profile					1708:1714	the only microbiota profile	1688:1714	the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1688:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	6	118	theme	microbiota	1697:1706	arg1	this					1679:1682	this	1679:1682	this	1679:1682	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	4	119	theme	valid	1187:1191	arg1	rRNA					1207:1210	2,186,596 valid bacterial 16S rRNA reads	1177:1216	2,186,596 valid bacterial 16S rRNA reads	1177:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	4	120	dep	RESULTS	1148:1154	arg1	library					1166:1172	A genomic library	1156:1172	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads	1148:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	8	121	theme	gut	2210:2212	arg1	microbiota					2214:2223	a polysaccharide fermenting gut microbiota	2182:2223	a polysaccharide fermenting gut microbiota	2182:2223	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
29636094	3	122	theme	microbiome	908:917	arg1	diversity					887:895	diversity	887:895	diversity	887:895	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	3	122	theme	microbiome	908:917	arg1	composition					871:881	the composition	867:881	the composition	867:881	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	6	123	theme	captive	1775:1781	arg1	turtles					1789:1795	the captive green turtles	1771:1795	the captive green turtles of any size feeding on a macroalgae/fish mixed diet	1771:1847	Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet.
29636094	5	124	theme	carapace	1435:1442	arg1	length					1444:1449	curved carapace length	1428:1449	curved carapace length	1428:1449	The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum.
29636094	4	125	theme	16S	1203:1205	arg1	rRNA					1207:1210	2,186,596 valid bacterial 16S rRNA reads	1177:1216	2,186,596 valid bacterial 16S rRNA reads	1177:1216	RESULTS A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff).
29636094	3	126	theme	diets	1096:1100	arg1	consumption					1070:1080	the consumption	1066:1080	the consumption of omnivorous diets	1066:1100	We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles.
29636094	8	127	theme	Brazil	2072:2077	arg1	waters					2062:2067	the coastal waters	2050:2067	the coastal waters of Brazil	2050:2077	CONCLUSIONS These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats.
32146468	3	0	theme	EP	305:306	arg1	production					325:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	2	1	theme	recent	115:120	arg1	years					122:126	recent years	115:126	recent years	115:126	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	5	2	theme	glycoside	504:512	arg1	hydrolases					514:523	glycoside hydrolases	504:523	glycoside hydrolases that have the potential to hydrolyze EP	504:563	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	6	3	theme	strain	596:601	arg1	capability					582:591	the capability	578:591	the capability of strain H11 to hydrolyze EP	578:621	To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized.
32146468	7	4	theme	Response	684:691	arg1	methodology					701:711	Response surface methodology	684:711	Response surface methodology analysis	684:720	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	7	5	theme	surface	693:699	arg1	methodology					701:711	Response surface methodology	684:711	Response surface methodology analysis	684:720	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	0	6	theme	sp	103:104	arg1	Enzymes					85:91	the Crude Enzymes	75:91	the Crude Enzymes of Vibrio sp	75:104	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	10	7	theme	system	1167:1172	arg1	application					1134:1144	The combined application	1121:1144	The combined application of the H11 enzymatic system and anaerobic fermentation	1121:1199	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	3	8	theme	hydrolase	346:354	arg1	system					356:361	the hydrolase system	342:361	the hydrolase system of Vibrio sp	342:374	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	8	9	theme	EP	831:832	arg1	g/L					824:826	50 g/L	821:826	50 g/L of EP	821:832	When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%.
32146468	0	10	from	Polysaccharides	56:70	arg1	Production					27:36	Enhanced Fermentable Sugar Production	0:36	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.	0:105	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	7	11	from	strain	768:773	arg1	production					752:761	maximal enzymatic production	734:761	maximal enzymatic production from strain H11	734:777	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	10	12	theme	anaerobic	1178:1186	arg1	fermentation					1188:1199	anaerobic fermentation	1178:1199	anaerobic fermentation	1178:1199	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	10	13	theme	comprehensive	1234:1246	arg1	application					1248:1258	the comprehensive application	1230:1258	the comprehensive application of EP	1230:1264	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	2	14	theme	large-scale	129:139	arg1	outbreaks					141:149	large-scale outbreaks	129:149	large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters	129:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	10	15	theme	EP	1263:1264	arg1	application					1248:1258	the comprehensive application	1230:1258	the comprehensive application of EP	1230:1264	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	3	16	theme	sp	373:374	arg1	system					356:361	the hydrolase system	342:361	the hydrolase system of Vibrio sp	342:374	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	9	17	theme	6.74	1025:1028	arg1	mL/L					1006:1009	3,217 mL/L	1000:1009	3,217 mL/L of biogas and 6.74 g/L of biosolvents	1000:1047	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	0	18	theme	Fermentable	9:19	arg1	Production					27:36	Enhanced Fermentable Sugar Production	0:36	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.	0:105	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	3	19	theme	enzymatic	309:317	arg1	production					325:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	0	20	theme	Enhanced	0:7	arg1	Production					27:36	Enhanced Fermentable Sugar Production	0:36	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.	0:105	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	10	21	theme	fermentation	1188:1199	arg1	application					1134:1144	The combined application	1121:1144	The combined application of the H11 enzymatic system and anaerobic fermentation	1121:1199	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	7	22	theme	26-h	799:802	arg1	incubation					804:813	26-h incubation	799:813	26-h incubation	799:813	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	9	23	theme	7.29	1078:1081	arg1	%					1082:1082	7.29%	1078:1082	7.29%	1078:1082	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	5	24	contain	contain	440:446	arg2	genes					473:477	711 carbohydrate-related genes	448:477	711 carbohydrate-related genes	448:477	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	5	24	contain	contain	440:446	arg1	Strain					416:421	Strain H11	416:425	Strain H11	416:425	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	6	25	theme	culture	633:639	arg1	medium					641:646	the culture medium	629:646	the culture medium	629:646	To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized.
32146468	0	26	theme	Sugar	21:25	arg1	Production					27:36	Enhanced Fermentable Sugar Production	0:36	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.	0:105	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	9	27	dep	biogas	1014:1019	arg1	g/L					1030:1032	g/L	1030:1032	g/L	1030:1032	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	2	28	theme	Enteromorpha	169:180	arg1	prolifera					182:190	the green alga Enteromorpha prolifera	154:190	the green alga Enteromorpha prolifera in China's offshore waters	154:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	8	29	theme	H11	858:860	arg1	enzyme					862:867	crude H11 enzyme	852:867	crude H11 enzyme	852:867	When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%.
32146468	8	30	theme	crude	852:856	arg1	enzyme					862:867	crude H11 enzyme	852:867	crude H11 enzyme	852:867	When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%.
32146468	0	31	theme	Enteromorpha	43:54	arg1	Polysaccharides					56:70	Enteromorpha Polysaccharides	43:70	Enteromorpha Polysaccharides	43:70	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	2	32	theme	alga	164:167	arg1	prolifera					182:190	the green alga Enteromorpha prolifera	154:190	the green alga Enteromorpha prolifera in China's offshore waters	154:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	5	33	theme	carbohydrate-related	452:471	arg1	genes					473:477	711 carbohydrate-related genes	448:477	711 carbohydrate-related genes	448:477	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	10	34	theme	combined	1125:1132	arg1	application					1134:1144	The combined application	1121:1144	The combined application of the H11 enzymatic system and anaerobic fermentation	1121:1199	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	2	35	theme	green	158:162	arg1	prolifera					182:190	the green alga Enteromorpha prolifera	154:190	the green alga Enteromorpha prolifera in China's offshore waters	154:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	9	36	theme	%	1082:1082	arg1	increases					1055:1063	increases	1055:1063	increases of 28.17 and 7.29%	1055:1082	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	3	37	theme	polysaccharide	289:302	arg1	production					325:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	7	38	theme	maximal	734:740	arg1	production					752:761	maximal enzymatic production	734:761	maximal enzymatic production from strain H11	734:777	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	4	39	theme	microbial	398:406	arg1	strain					408:413	an EP-utilizing microbial strain	382:413	an EP-utilizing microbial strain	382:413	H11, an EP-utilizing microbial strain.
32146468	2	40	theme	offshore	203:210	arg1	waters					212:217	China's offshore waters	195:217	China's offshore waters	195:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	9	41	theme	28.17	1068:1072	arg1	increases					1055:1063	increases	1055:1063	increases of 28.17 and 7.29%	1055:1082	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	5	42	contain	have	530:533	arg2	potential					539:547	the potential to hydrolyze EP	535:563	the potential to hydrolyze EP	535:563	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	5	42	contain	have	530:533	arg1	hydrolases					514:523	glycoside hydrolases	504:523	glycoside hydrolases that have the potential to hydrolyze EP	504:563	Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP.
32146468	10	43	theme	enzymatic	1157:1165	arg1	system					1167:1172	the H11 enzymatic system	1149:1172	the H11 enzymatic system	1149:1172	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	7	44	theme	enzymatic	742:750	arg1	production					752:761	maximal enzymatic production	734:761	maximal enzymatic production from strain H11	734:777	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	4	45	theme	EP-utilizing	385:396	arg1	strain					408:413	an EP-utilizing microbial strain	382:413	an EP-utilizing microbial strain	382:413	H11, an EP-utilizing microbial strain.
32146468	10	46	theme	H11	1153:1155	arg1	system					1167:1172	the H11 enzymatic system	1149:1172	the H11 enzymatic system	1149:1172	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	3	47	theme	Enteromorpha	276:287	arg1	production					325:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	8	48	theme	sugars	904:909	arg1	concentration					874:886	the concentration	870:886	the concentration of fermentation sugars	870:909	When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%.
32146468	9	49	theme	biosolvents	1037:1047	arg1	6.74					1025:1028	6.74	1025:1028	6.74	1025:1028	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	9	49	theme	biosolvents	1037:1047	arg1	biogas					1014:1019	biogas	1014:1019	biogas	1014:1019	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	4	50	dep	strain	408:413	arg1	H11					377:379	H11	377:379	H11	377:379	H11, an EP-utilizing microbial strain.
32146468	0	51	theme	Crude	79:83	arg1	Enzymes					85:91	the Crude Enzymes	75:91	the Crude Enzymes of Vibrio sp	75:104	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
32146468	2	52	theme	prolifera	182:190	arg1	outbreaks					141:149	large-scale outbreaks	129:149	large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters	129:217	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	2	53	theme	serious	232:238	arg1	threat					240:245	a serious threat	230:245	a serious threat	230:245	In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat.
32146468	8	54	theme	fermentation	891:902	arg1	sugars					904:909	fermentation sugars	891:909	fermentation sugars	891:909	When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%.
32146468	7	55	theme	methodology	701:711	arg1	analysis					713:720	Response surface methodology analysis	684:720	Response surface methodology analysis	684:720	Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation.
32146468	3	56	theme	sugar	319:323	arg1	production					325:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	Enteromorpha polysaccharide (EP) enzymatic sugar production	276:334	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	10	57	contain	has	1201:1203	arg2	potential					1209:1217	the potential to improve the comprehensive application of EP	1205:1264	the potential to improve the comprehensive application of EP	1205:1264	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	10	57	contain	has	1201:1203	arg1	application					1134:1144	The combined application	1121:1144	The combined application of the H11 enzymatic system and anaerobic fermentation	1121:1199	The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
32146468	3	58	theme	Vibrio	366:371	arg1	sp					373:374	Vibrio sp	366:374	Vibrio sp	366:374	This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp.
32146468	9	59	theme	biogas	1014:1019	arg1	mL/L					1006:1009	3,217 mL/L	1000:1009	3,217 mL/L of biogas and 6.74 g/L of biosolvents	1000:1047	Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls.
32146468	0	60	theme	Vibrio	96:101	arg1	sp					103:104	Vibrio sp	96:104	Vibrio sp	96:104	Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp.
31101261	7	0	theme	composite	1232:1240	arg1	H2O2					1269:1272	the GR-guar/CuO composite modified electrode detects H2O2	1216:1272	the GR-guar/CuO composite modified electrode detects H2O2 in the response	1216:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	1	1	theme	materials	278:286	arg1	synthesis					216:224	A novel and cost-effective synthesis	189:224	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials	189:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	6	2	theme	voltammetric	954:965	arg1	studies					967:973	Cyclic voltammetric studies	947:973	Cyclic voltammetric studies	947:973	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	4	3	theme	simple	713:718	arg1	method					733:738	A simple sonochemical method	711:738	A simple sonochemical method	711:738	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	9	4	theme	H2O2	1535:1538	arg1	detection					1522:1530	the detection	1518:1530	the detection of H2O2	1518:1538	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	3	5	theme	physicochemical	566:580	arg1	methods					599:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	7	6	theme	optimized	1181:1189	arg1	conditions					1204:1213	optimized experimental conditions	1181:1213	optimized experimental conditions	1181:1213	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	1	7	theme	novel	191:195	arg1	synthesis					216:224	A novel and cost-effective synthesis	189:224	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials	189:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	8	8	theme	5.8 nM	1365:1370	arg1	limit					1356:1360	a lower detection limit	1338:1360	a lower detection limit of 5.8 nM with high sensitivity	1338:1392	The sensor shows a lower detection limit of 5.8 nM with high sensitivity.
31101261	0	9	theme	electrochemical	119:133	arg1	detection					135:143	enhanced electrochemical detection	110:143	enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples	110:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	1	10	theme	cost-effective	201:214	arg1	synthesis					216:224	A novel and cost-effective synthesis	189:224	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials	189:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	6	11	theme	screen-printed	1024:1037	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	11	theme	screen-printed	1024:1037	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	8	12	theme	high	1377:1380	arg1	sensitivity					1382:1392	high sensitivity	1377:1392	high sensitivity	1377:1392	The sensor shows a lower detection limit of 5.8 nM with high sensitivity.
31101261	5	13	theme	stabilized	864:873	arg1	CuO-guar					880:887	CuO-guar	880:887	CuO-guar	880:887	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	5	13	theme	stabilized	864:873	arg1	CuO					875:877	stabilized CuO	864:877	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	6	14	theme	composite	1005:1013	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	14	theme	composite	1005:1013	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	3	15	theme	successful	632:641	arg1	exfoliation					643:653	the successful exfoliation	628:653	the successful exfoliation of graphite and formation of the GR-guar/CuO composite	628:708	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	4	16	theme	guar	777:780	arg1	graphite					804:811	guar stabilized exfoliated graphite	777:811	guar stabilized exfoliated graphite (GR-guar)	777:821	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	4	16	theme	guar	777:780	arg1	GR-guar					814:820	GR-guar	814:820	GR-guar	814:820	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	6	17	theme	electro-reduction	1076:1092	arg1	ability					1094:1100	enhanced electro-reduction ability	1067:1100	enhanced electro-reduction ability towards H2O2	1067:1113	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	2	18	theme	exfoliated	511:520	arg1	composite					545:553	exfoliated graphite (GR-guar/CuO) composite	511:553	exfoliated graphite (GR-guar/CuO) composite	511:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	0	19	theme	exfoliated	73:82	arg1	graphite					84:91	exfoliated graphite	73:91	exfoliated graphite	73:91	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	1	20	theme	small	382:386	arg1	molecules					388:396	small molecules	382:396	small molecules	382:396	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	7	21	from	H2O2	1269:1272	arg1	response					1281:1288	the response	1277:1288	the response	1277:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	10	22	theme	fabricated	1559:1568	arg1	sensor					1570:1575	the fabricated sensor	1555:1575	the fabricated sensor	1555:1575	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	6	23	dep	GR-guar	1120:1126	arg1	SPCEs					1168:1172	graphite/CuO-guar modified SPCEs	1141:1172	graphite/CuO-guar modified SPCEs	1141:1172	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	9	24	theme	GR-guar/CuO	1411:1421	arg1	sensor					1433:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	24	theme	GR-guar/CuO	1411:1421	arg1	reproducible					1450:1461	reproducible	1450:1461	reproducible	1450:1461	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	5	25	theme	flower-like	828:838	arg1	CuO					840:842	The flower-like CuO	824:842	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites	824:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	4	26	used	used	749:752	arg2	method					733:738	A simple sonochemical method	711:738	A simple sonochemical method	711:738	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	2	27	theme	biopolymer	463:472	arg1	synthesis					434:442	the synthesis	430:442	the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite	430:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	4	28	theme	exfoliated	793:802	arg1	graphite					804:811	guar stabilized exfoliated graphite	777:811	guar stabilized exfoliated graphite (GR-guar)	777:821	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	4	28	theme	exfoliated	793:802	arg1	GR-guar					814:820	GR-guar	814:820	GR-guar	814:820	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	0	29	theme	Sonochemical	0:11	arg1	synthesis					13:21	Sonochemical synthesis	0:21	Sonochemical synthesis of gum guar biopolymer	0:44	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	1	30	theme	substantial	302:312	arg1	attention					314:322	substantial attention	302:322	substantial attention	302:322	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	6	31	theme	graphite/CuO-guar	1141:1157	arg1	SPCEs					1168:1172	graphite/CuO-guar modified SPCEs	1141:1172	graphite/CuO-guar modified SPCEs	1141:1172	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	10	32	theme	H2O2	1619:1622	arg1	detection					1606:1614	the accurate detection	1593:1614	the accurate detection of H2O2 in real samples	1593:1638	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	6	33	contain	had	1063:1065	arg2	ability					1094:1100	enhanced electro-reduction ability	1067:1100	enhanced electro-reduction ability towards H2O2	1067:1113	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	33	contain	had	1063:1065	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	33	contain	had	1063:1065	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	2	34	theme	guar	451:454	arg1	gum					447:449	gum guar	447:454	gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite	447:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	2	34	theme	guar	451:454	arg1	guar					457:460	guar	457:460	guar	457:460	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	5	35	theme	CuO	875:877	arg1	composites					890:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	0	36	theme	guar	30:33	arg1	biopolymer					35:44	gum guar biopolymer	26:44	gum guar biopolymer	26:44	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	1	37	theme	broad	329:333	arg1	range					335:339	a broad range	327:339	a broad range application including electroanalysis of small molecules	327:396	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	0	38	dep	stabilized	46:55	arg1	Application					94:104	Application	94:104	Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples	94:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	2	39	theme	cupric	485:490	arg1	oxide					492:496	cupric oxide	485:496	cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite	485:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	9	40	theme	excellent	1471:1479	arg1	selectivity					1481:1491	excellent selectivity	1471:1491	excellent selectivity	1471:1491	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	3	41	theme	GR-guar/CuO	688:698	arg1	composite					700:708	the GR-guar/CuO composite	684:708	the GR-guar/CuO composite	684:708	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	7	42	theme	electrode	1251:1259	arg1	H2O2					1269:1272	the GR-guar/CuO composite modified electrode detects H2O2	1216:1272	the GR-guar/CuO composite modified electrode detects H2O2 in the response	1216:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	10	43	theme	real	1627:1630	arg1	samples					1632:1638	real samples	1627:1638	real samples	1627:1638	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	1	44	theme	biopolymer-based	229:244	arg1	materials					278:286	biopolymer-based organic and inorganic composite materials	229:286	biopolymer-based organic and inorganic composite materials	229:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	0	45	theme	copper	57:62	arg1	oxide					64:68	copper oxide	57:68	copper oxide	57:68	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	7	46	theme	GR-guar/CuO	1220:1230	arg1	H2O2					1269:1272	the GR-guar/CuO composite modified electrode detects H2O2	1216:1272	the GR-guar/CuO composite modified electrode detects H2O2 in the response	1216:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	1	47	theme	composite	268:276	arg1	materials					278:286	biopolymer-based organic and inorganic composite materials	229:286	biopolymer-based organic and inorganic composite materials	229:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	3	48	theme	characterization	582:597	arg1	methods					599:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	6	49	theme	Cyclic	947:952	arg1	studies					967:973	Cyclic voltammetric studies	947:973	Cyclic voltammetric studies	947:973	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	7	50	theme	experimental	1191:1202	arg1	conditions					1204:1213	optimized experimental conditions	1181:1213	optimized experimental conditions	1181:1213	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	3	51	theme	Different	556:564	arg1	methods					599:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	5	52	theme	GR-guar	847:853	arg1	composites					890:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	8	53	theme	detection	1346:1354	arg1	limit					1356:1360	a lower detection limit	1338:1360	a lower detection limit of 5.8 nM with high sensitivity	1338:1392	The sensor shows a lower detection limit of 5.8 nM with high sensitivity.
31101261	6	54	theme	carbon	1039:1044	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	54	theme	carbon	1039:1044	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	0	55	theme	H2O2	148:151	arg1	detection					135:143	enhanced electrochemical detection	110:143	enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples	110:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	4	56	theme	sonochemical	720:731	arg1	method					733:738	A simple sonochemical method	711:738	A simple sonochemical method	711:738	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	6	57	theme	modified	1015:1022	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	57	theme	modified	1015:1022	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	5	58	from	CuO	840:842	arg1	composites					890:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	6	59	theme	GR-guar/CuO	993:1003	arg1	SPCE					1057:1060	SPCE	1057:1060	SPCE	1057:1060	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	6	59	theme	GR-guar/CuO	993:1003	arg1	electrode					1046:1054	the GR-guar/CuO composite modified screen-printed carbon electrode	989:1054	the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE)	989:1061	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	0	60	theme	pharmaceutical	165:178	arg1	samples					180:186	pharmaceutical samples	165:186	pharmaceutical samples	165:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	3	61	theme	formation	671:679	arg1	exfoliation					643:653	the successful exfoliation	628:653	the successful exfoliation of graphite and formation of the GR-guar/CuO composite	628:708	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	0	62	theme	biopolymer	35:44	arg1	synthesis					13:21	Sonochemical synthesis	0:21	Sonochemical synthesis of gum guar biopolymer	0:44	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	10	63	from	detection	1606:1614	arg1	samples					1632:1638	real samples	1627:1638	real samples	1627:1638	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	3	64	theme	graphite	658:665	arg1	exfoliation					643:653	the successful exfoliation	628:653	the successful exfoliation of graphite and formation of the GR-guar/CuO composite	628:708	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	3	65	used	used	612:615	arg2	methods					599:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods	556:605	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	5	66	theme	hydrothermal	926:937	arg1	method					939:944	a hydrothermal method	924:944	a hydrothermal method	924:944	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	2	67	theme	graphite	522:529	arg1	composite					545:553	exfoliated graphite (GR-guar/CuO) composite	511:553	exfoliated graphite (GR-guar/CuO) composite	511:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	6	68	theme	enhanced	1067:1074	arg1	ability					1094:1100	enhanced electro-reduction ability	1067:1100	enhanced electro-reduction ability towards H2O2	1067:1113	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	1	69	theme	molecules	388:396	arg1	electroanalysis					363:377	electroanalysis	363:377	electroanalysis of small molecules	363:396	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	9	70	theme	as-prepared	1399:1409	arg1	sensor					1433:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	70	theme	as-prepared	1399:1409	arg1	reproducible					1450:1461	reproducible	1450:1461	reproducible	1450:1461	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	0	71	theme	enhanced	110:117	arg1	detection					135:143	enhanced electrochemical detection	110:143	enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples	110:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	9	72	theme	composite	1423:1431	arg1	sensor					1433:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	72	theme	composite	1423:1431	arg1	reproducible					1450:1461	reproducible	1450:1461	reproducible	1450:1461	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	2	73	theme	GR-guar/CuO	532:542	arg1	composite					545:553	exfoliated graphite (GR-guar/CuO) composite	511:553	exfoliated graphite (GR-guar/CuO) composite	511:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	6	74	theme	modified	1159:1166	arg1	SPCEs					1168:1172	graphite/CuO-guar modified SPCEs	1141:1172	graphite/CuO-guar modified SPCEs	1141:1172	Cyclic voltammetric studies revealed that the GR-guar/CuO composite modified screen-printed carbon electrode (SPCE) had enhanced electro-reduction ability towards H2O2 than GR-guar and pristine graphite/CuO-guar modified SPCEs.
31101261	8	75	theme	lower	1340:1344	arg1	limit					1356:1360	a lower detection limit	1338:1360	a lower detection limit of 5.8 nM with high sensitivity	1338:1392	The sensor shows a lower detection limit of 5.8 nM with high sensitivity.
31101261	4	76	theme	stabilized	782:791	arg1	graphite					804:811	guar stabilized exfoliated graphite	777:811	guar stabilized exfoliated graphite (GR-guar)	777:821	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	4	76	theme	stabilized	782:791	arg1	GR-guar					814:820	GR-guar	814:820	GR-guar	814:820	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	0	77	theme	gum	26:28	arg1	biopolymer					35:44	gum guar biopolymer	26:44	gum guar biopolymer	26:44	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	5	78	theme	guar	859:862	arg1	composites					890:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	GR-guar and guar stabilized CuO (CuO-guar) composites	847:899	The flower-like CuO on GR-guar and guar stabilized CuO (CuO-guar) composites were synthesized using a hydrothermal method.
31101261	3	79	theme	composite	700:708	arg1	formation					671:679	formation	671:679	formation	671:679	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	3	79	theme	composite	700:708	arg1	graphite					658:665	graphite	658:665	graphite	658:665	Different physicochemical characterization methods were used to confirm the successful exfoliation of graphite and formation of the GR-guar/CuO composite.
31101261	4	80	theme	graphite	804:811	arg1	preparation					762:772	the preparation	758:772	the preparation of guar stabilized exfoliated graphite (GR-guar)	758:821	A simple sonochemical method has been used for the preparation of guar stabilized exfoliated graphite (GR-guar).
31101261	2	81	theme	gum	447:449	arg1	biopolymer					463:472	gum guar (guar) biopolymer	447:472	gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite	447:553	In this perspective, we report the synthesis of gum guar (guar) biopolymer stabilized cupric oxide decorated on exfoliated graphite (GR-guar/CuO) composite.
31101261	7	82	theme	detects	1261:1267	arg1	H2O2					1269:1272	the GR-guar/CuO composite modified electrode detects H2O2	1216:1272	the GR-guar/CuO composite modified electrode detects H2O2 in the response	1216:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	10	83	used	used	1584:1587	arg2	sensor					1570:1575	the fabricated sensor	1555:1575	the fabricated sensor	1555:1575	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	10	84	theme	accurate	1597:1604	arg1	detection					1606:1614	the accurate detection	1593:1614	the accurate detection of H2O2 in real samples	1593:1638	Consequently, the fabricated sensor can be used for the accurate detection of H2O2 in real samples.
31101261	9	85	contain	had	1467:1469	arg2	practicality					1497:1508	practicality	1497:1508	practicality	1497:1508	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	85	contain	had	1467:1469	arg2	selectivity					1481:1491	excellent selectivity	1471:1491	excellent selectivity	1471:1491	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	85	contain	had	1467:1469	arg1	sensor					1433:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor	1395:1438	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	9	85	contain	had	1467:1469	arg1	reproducible					1450:1461	reproducible	1450:1461	reproducible	1450:1461	The as-prepared GR-guar/CuO composite sensor is highly reproducible and had excellent selectivity and practicality towards the detection of H2O2.
31101261	7	86	theme	modified	1242:1249	arg1	H2O2					1269:1272	the GR-guar/CuO composite modified electrode detects H2O2	1216:1272	the GR-guar/CuO composite modified electrode detects H2O2 in the response	1216:1288	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	1	87	theme	range	335:339	arg1	application					341:351	a broad range application	327:351	a broad range application including electroanalysis of small molecules	327:396	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	7	88	dep	1296.6 µM	1310:1318	arg1	to					1307:1308	to	1307:1308	to	1307:1308	Under optimized experimental conditions, the GR-guar/CuO composite modified electrode detects H2O2 in the response ranges from 0.02 to 1296.6 µM.
31101261	1	89	theme	organic	246:252	arg1	materials					278:286	biopolymer-based organic and inorganic composite materials	229:286	biopolymer-based organic and inorganic composite materials	229:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
31101261	0	90	from	detection	135:143	arg1	milk					156:159	milk	156:159	milk	156:159	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	0	90	from	detection	135:143	arg1	samples					180:186	pharmaceutical samples	165:186	pharmaceutical samples	165:186	Sonochemical synthesis of gum guar biopolymer stabilized copper oxide on exfoliated graphite: Application for enhanced electrochemical detection of H2O2 in milk and pharmaceutical samples.
31101261	8	91	with	limit	1356:1360	arg1	sensitivity					1382:1392	high sensitivity	1377:1392	high sensitivity	1377:1392	The sensor shows a lower detection limit of 5.8 nM with high sensitivity.
31101261	1	92	theme	inorganic	258:266	arg1	materials					278:286	biopolymer-based organic and inorganic composite materials	229:286	biopolymer-based organic and inorganic composite materials	229:286	A novel and cost-effective synthesis of biopolymer-based organic and inorganic composite materials have received substantial attention in a broad range application including electroanalysis of small molecules.
30527747	0	0	theme	dendritic	104:112	arg1	cells					114:118	dendritic cells	104:118	dendritic cells	104:118	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	2	1	from	isolate	444:450	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	2	theme	tuberculosis	361:372	arg1	H37Rv					374:378	Mycobacterium tuberculosis H37Rv	347:378	Mycobacterium tuberculosis H37Rv	347:378	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	4	3	theme	key	750:752	arg1	role					754:757	a key role	748:757	a key role	748:757	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	5	4	theme	novel	1108:1112	arg1	strategy					1143:1150	a novel polysaccharide immunological strategy	1106:1150	a novel polysaccharide immunological strategy against tuberculosis	1106:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	5	5	theme	polysaccharide	1114:1127	arg1	strategy					1143:1150	a novel polysaccharide immunological strategy	1106:1150	a novel polysaccharide immunological strategy against tuberculosis	1106:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	6	theme	Mycobacterium	347:359	arg1	H37Rv					374:378	Mycobacterium tuberculosis H37Rv	347:378	Mycobacterium tuberculosis H37Rv	347:378	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	4	7	theme	LAM	732:734	arg1	end					725:727	the terminal end	712:727	the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection	712:832	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	5	8	theme	immunological	1129:1141	arg1	strategy					1143:1150	a novel polysaccharide immunological strategy	1106:1150	a novel polysaccharide immunological strategy against tuberculosis	1106:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	9	theme	structural	283:292	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	0	10	theme	cells	114:118	arg1	activation					90:99	activation	90:99	activation	90:99	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	0	10	theme	cells	114:118	arg1	maturation					75:84	maturation	75:84	maturation	75:84	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	4	11	theme	residues	688:695	arg1	difference					637:646	the major structural difference	616:646	the major structural difference of these LAMs	616:660	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	11	theme	residues	688:695	arg1	amount					669:674	the amount	665:674	the amount of mannosyl residues	665:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	11	theme	residues	688:695	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	12	theme	terminal	716:723	arg1	end					725:727	the terminal end	712:727	the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection	712:832	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	2	13	from	strains	338:344	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	4	14	theme	mycobacterial	810:822	arg1	infection					824:832	mycobacterial infection	810:832	mycobacterial infection	810:832	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	0	15	theme	Mannosylated	0:11	arg1	structures					13:22	Mannosylated structures	0:22	Mannosylated structures of mycobacterial lipoarabinomannans	0:58	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	3	16	theme	immunomodulatory	563:578	arg1	properties					580:589	the immunomodulatory properties	559:589	the immunomodulatory properties	559:589	QGD101, was analyzed and further evaluated whether these LAMs can induce DC maturation and promote the immunomodulatory properties.
30527747	2	17	theme	M.	453:454	arg1	isolate					444:450	a newly discovered clinical isolate	416:450	a newly discovered clinical isolate	416:450	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	17	theme	M.	453:454	arg1	sp					456:457	M. sp	453:457	M. sp	453:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	18	theme	LAMs	308:311	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	19	theme	Mycobacterium	381:393	arg1	smegmatis					395:403	Mycobacterium smegmatis mc2155	381:410	Mycobacterium smegmatis mc2155	381:410	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	0	20	theme	mycobacterial	27:39	arg1	lipoarabinomannans					41:58	mycobacterial lipoarabinomannans	27:58	mycobacterial lipoarabinomannans	27:58	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	5	21	gly	glycosylated	897:908	arg1	structure					910:918	the glycosylated structure	893:918	the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis	893:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	22	from	H37Rv	374:378	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	23	theme	clinical	435:442	arg1	isolate					444:450	a newly discovered clinical isolate	416:450	a newly discovered clinical isolate	416:450	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	23	theme	clinical	435:442	arg1	sp					456:457	M. sp	453:457	M. sp	453:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	5	24	theme	immunomodulatory	935:950	arg1	property					952:959	its immunomodulatory property	931:959	its immunomodulatory property	931:959	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	25	theme	discovered	424:433	arg1	isolate					444:450	a newly discovered clinical isolate	416:450	a newly discovered clinical isolate	416:450	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	2	25	theme	discovered	424:433	arg1	sp					456:457	M. sp	453:457	M. sp	453:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	5	26	theme	glycosylated	897:908	arg1	structure					910:918	the glycosylated structure	893:918	the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis	893:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	5	27	theme	potential	992:1000	arg1	approach					1002:1009	a potential approach	990:1009	a potential approach for identification of different mycobacteria	990:1054	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	4	28	theme	LAMs	657:660	arg1	difference					637:646	the major structural difference	616:646	the major structural difference of these LAMs	616:660	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	28	theme	LAMs	657:660	arg1	amount					669:674	the amount	665:674	the amount of mannosyl residues	665:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	28	theme	LAMs	657:660	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	0	29	theme	lipoarabinomannans	41:58	arg1	structures					13:22	Mannosylated structures	0:22	Mannosylated structures of mycobacterial lipoarabinomannans	0:58	Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.
30527747	5	30	theme	strategy	1143:1150	arg1	development					1091:1101	the development	1087:1101	the development of a novel polysaccharide immunological strategy against tuberculosis	1087:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	31	from	smegmatis	395:403	arg1	difference					294:303	the structural difference	279:303	the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp	279:457	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	1	32	theme	strong	228:233	arg1	response					242:249	a strong immune response	226:249	a strong immune response	226:249	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	4	33	theme	DCs	800:802	arg1	response					788:795	the divergent response	774:795	the divergent response of DCs	774:802	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	2	34	dep	difference	294:303	arg1	study					272:276	this study	267:276	this study	267:276	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	4	35	from	end	725:727	arg1	difference					637:646	the major structural difference	616:646	the major structural difference of these LAMs	616:660	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	35	from	end	725:727	arg1	amount					669:674	the amount	665:674	the amount of mannosyl residues	665:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	35	from	end	725:727	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	5	36	theme	LAM	923:925	arg1	structure					910:918	the glycosylated structure	893:918	the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis	893:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	5	37	theme	different	1033:1041	arg1	mycobacteria					1043:1054	different mycobacteria	1033:1054	different mycobacteria	1033:1054	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	2	38	theme	mycobacterial	324:336	arg1	strains					338:344	three mycobacterial strains	318:344	three mycobacterial strains	318:344	In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc2155 and a newly discovered clinical isolate, M. sp.
30527747	1	39	theme	immune	235:240	arg1	response					242:249	a strong immune response	226:249	a strong immune response	226:249	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	3	40	theme	DC	533:534	arg1	maturation					536:545	DC maturation	533:545	DC maturation	533:545	QGD101, was analyzed and further evaluated whether these LAMs can induce DC maturation and promote the immunomodulatory properties.
30527747	5	41	theme	mycobacteria	1043:1054	arg1	identification					1015:1028	identification	1015:1028	identification of different mycobacteria	1015:1054	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	5	42	theme	property	952:959	arg1	structure					910:918	the glycosylated structure	893:918	the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis	893:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	4	43	theme	mannosyl	679:686	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	5	44	theme	important	865:873	arg1	relevance					875:883	an important relevance	862:883	an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis	862:1171	Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.
30527747	4	45	theme	divergent	778:786	arg1	response					788:795	the divergent response	774:795	the divergent response of DCs	774:802	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	1	46	theme	important	151:159	arg1	Lipoarabinomannan					121:137	Lipoarabinomannan	121:137	Lipoarabinomannan (LAM)	121:143	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	1	46	theme	important	151:159	arg1	factor					170:175	an important virulent factor	148:175	an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host	148:261	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	4	47	theme	structural	626:635	arg1	difference					637:646	the major structural difference	616:646	the major structural difference of these LAMs	616:660	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	47	theme	structural	626:635	arg1	amount					669:674	the amount	665:674	the amount of mannosyl residues	665:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	47	theme	structural	626:635	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	1	48	theme	virulent	161:168	arg1	Lipoarabinomannan					121:137	Lipoarabinomannan	121:137	Lipoarabinomannan (LAM)	121:143	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	1	48	theme	virulent	161:168	arg1	factor					170:175	an important virulent factor	148:175	an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host	148:261	Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host.
30527747	4	49	theme	major	620:624	arg1	difference					637:646	the major structural difference	616:646	the major structural difference of these LAMs	616:660	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	49	theme	major	620:624	arg1	amount					669:674	the amount	665:674	the amount of mannosyl residues	665:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
30527747	4	49	theme	major	620:624	arg1	residues					688:695	mannosyl residues	679:695	mannosyl residues	679:695	The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection.
29476852	2	0	theme	ultrasonic-assisted	473:491	arg1	extraction					493:502	ultrasonic-assisted extraction	473:502	ultrasonic-assisted extraction (UAM)	473:508	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	2	0	theme	ultrasonic-assisted	473:491	arg1	UAM					505:507	UAM	505:507	UAM	505:507	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	7	1	theme	mucilage	1155:1162	arg1	characteristics					1129:1143	the basic characteristics	1119:1143	the basic characteristics of native mucilage in the carpel of N. odorata	1119:1190	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	4	2	theme	in	669:670	arg1	activity					690:697	in vitro antioxidant activity	669:697	in vitro antioxidant activity	669:697	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	1	3	theme	mucilage	241:248	arg1	amount					219:224	a large amount	211:224	a large amount of transparent mucilage	211:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	1	3	theme	mucilage	241:248	arg1	mucilage					241:248	transparent mucilage	229:248	transparent mucilage	229:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	0	4	theme	mucilage	74:81	arg1	properties					56:65	the physicochemical and functional properties	21:65	the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	21:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	0	5	from	study	12:16	arg1	properties					56:65	the physicochemical and functional properties	21:65	the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	21:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	5	6	theme	methylation	988:998	arg1	degree					978:983	the degree	974:983	the degree of methylation	974:998	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	5	6	theme	methylation	988:998	arg1	different					1004:1012	different	1004:1012	different	1004:1012	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	7	7	theme	N.	1181:1182	arg1	odorata					1184:1190	N. odorata	1181:1190	N. odorata	1181:1190	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	4	8	dep	in	669:670	arg1	vitro					672:676	vitro	672:676	vitro	672:676	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	7	9	from	characteristics	1129:1143	arg1	carpel					1171:1176	the carpel	1167:1176	the carpel of N. odorata	1167:1190	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	7	10	theme	native	1148:1153	arg1	mucilage					1155:1162	native mucilage	1148:1162	native mucilage	1148:1162	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	0	11	from	properties	56:65	arg1	carpel					90:95	the carpel	86:95	the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	86:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	5	12	theme	major	905:909	arg1	differences					920:930	major spectral differences	905:930	major spectral differences	905:930	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	4	13	theme	lower	713:717	arg1	viscosity					719:727	a lower viscosity	711:727	a lower viscosity	711:727	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	0	14	theme	Nymphaea	100:107	arg1	odorata					109:115	Nymphaea odorata	100:115	Nymphaea odorata using ultrasonic and classical heating extractions	100:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	5	15	theme	infrared	796:803	arg1	spectra					805:811	infrared spectra	796:811	infrared spectra	796:811	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	2	16	theme	mucilage	405:412	arg1	properties					386:395	the physicochemical and functional properties	351:395	the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM)	351:508	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	4	17	theme	water-holding	733:745	arg1	capacity					747:754	water-holding capacity	733:754	water-holding capacity	733:754	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	5	18	theme	dialyzed	831:838	arg1	HWM					840:842	the dialyzed HWM	827:842	the dialyzed HWM	827:842	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	2	19	theme	hot	442:444	arg1	HWM					464:466	HWM	464:466	HWM	464:466	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	2	19	theme	hot	442:444	arg1	extraction					452:461	conventional hot water extraction	429:461	conventional hot water extraction (HWM)	429:467	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	1	20	theme	cooked	173:178	arg1	carpel					180:185	The cooked carpel	169:185	The cooked carpel of Nymphaea odorata	169:205	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	2	21	theme	water	446:450	arg1	HWM					464:466	HWM	464:466	HWM	464:466	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	2	21	theme	water	446:450	arg1	extraction					452:461	conventional hot water extraction	429:461	conventional hot water extraction (HWM)	429:467	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	4	22	theme	yield	625:629	arg1	production					631:640	a higher yield production	616:640	a higher yield production	616:640	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	5	23	contain	have	900:903	arg2	differences					920:930	major spectral differences	905:930	major spectral differences	905:930	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	5	23	contain	have	900:903	arg1	UAM					848:850	UAM	848:850	UAM	848:850	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	5	23	contain	have	900:903	arg1	HWM					840:842	the dialyzed HWM	827:842	the dialyzed HWM	827:842	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	4	24	theme	phenol	643:648	arg1	concentration					650:662	phenol concentration	643:662	phenol concentration	643:662	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	0	25	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.	0:167	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	4	26	contain	had	707:709	arg2	capacity					747:754	water-holding capacity	733:754	water-holding capacity	733:754	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	4	26	contain	had	707:709	arg2	viscosity					719:727	a lower viscosity	711:727	a lower viscosity	711:727	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	4	26	contain	had	707:709	arg1	it					704:705	it	704:705	it	704:705	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	3	27	theme	mouse	557:561	arg1	NIH/3 T3					581:588	NIH/3 T3	581:588	NIH/3 T3	581:588	Neither HWM nor UAM affected the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL.
29476852	3	27	theme	mouse	557:561	arg1	fibroblasts					568:578	mouse skin fibroblasts	557:578	mouse skin fibroblasts (NIH/3 T3)	557:589	Neither HWM nor UAM affected the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL.
29476852	2	28	theme	physicochemical	355:369	arg1	properties					386:395	the physicochemical and functional properties	351:395	the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM)	351:508	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	2	29	theme	conventional	429:440	arg1	HWM					464:466	HWM	464:466	HWM	464:466	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	2	29	theme	conventional	429:440	arg1	extraction					452:461	conventional hot water extraction	429:461	conventional hot water extraction (HWM)	429:467	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	1	30	theme	basic	264:268	arg1	characteristics					270:284	the basic characteristics	260:284	the basic characteristics of this mucilage	260:301	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	6	31	theme	weight	1077:1082	arg1	polysaccharides					1084:1098	low-molecular weight polysaccharides	1063:1098	low-molecular weight polysaccharides	1063:1098	Compared to HWMD, UAMD in low-molecular weight polysaccharides increased.
29476852	1	32	theme	Nymphaea	190:197	arg1	odorata					199:205	Nymphaea odorata	190:205	Nymphaea odorata	190:205	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	8	33	theme	main	1305:1308	arg1	composition					1310:1320	the main composition	1301:1320	the main composition of HWMD and UAMD	1301:1337	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	8	33	theme	main	1305:1308	arg1	acid					1292:1295	glucuronic acid	1281:1295	glucuronic acid	1281:1295	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	0	34	theme	odorata	109:115	arg1	carpel					90:95	the carpel	86:95	the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	86:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	6	35	theme	low-molecular	1063:1075	arg1	polysaccharides					1084:1098	low-molecular weight polysaccharides	1063:1098	low-molecular weight polysaccharides	1063:1098	Compared to HWMD, UAMD in low-molecular weight polysaccharides increased.
29476852	1	36	theme	odorata	199:205	arg1	carpel					180:185	The cooked carpel	169:185	The cooked carpel of Nymphaea odorata	169:205	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	0	37	from	mucilage	74:81	arg1	carpel					90:95	the carpel	86:95	the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	86:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	8	38	theme	UAMD	1334:1337	arg1	composition					1310:1320	the main composition	1301:1320	the main composition of HWMD and UAMD	1301:1337	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	8	38	theme	UAMD	1334:1337	arg1	acid					1292:1295	glucuronic acid	1281:1295	glucuronic acid	1281:1295	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	4	39	theme	higher	618:623	arg1	production					631:640	a higher yield production	616:640	a higher yield production	616:640	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	0	40	theme	physicochemical	25:39	arg1	properties					56:65	the physicochemical and functional properties	21:65	the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	21:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	0	41	theme	ultrasonic	123:132	arg1	extractions					156:166	ultrasonic and classical heating extractions	123:166	extractions	156:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	1	42	theme	mucilage	294:301	arg1	characteristics					270:284	the basic characteristics	260:284	the basic characteristics of this mucilage	260:301	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	0	43	theme	heating	148:154	arg1	extractions					156:166	ultrasonic and classical heating extractions	123:166	extractions	156:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	4	44	contain	had	612:614	arg2	concentration					650:662	phenol concentration	643:662	phenol concentration	643:662	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	4	44	contain	had	612:614	arg1	UAM					608:610	UAM	608:610	UAM	608:610	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	4	44	contain	had	612:614	arg2	activity					690:697	in vitro antioxidant activity	669:697	in vitro antioxidant activity	669:697	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	4	44	contain	had	612:614	arg2	production					631:640	a higher yield production	616:640	a higher yield production	616:640	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	3	45	theme	skin	563:566	arg1	NIH/3 T3					581:588	NIH/3 T3	581:588	NIH/3 T3	581:588	Neither HWM nor UAM affected the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL.
29476852	3	45	theme	skin	563:566	arg1	fibroblasts					568:578	mouse skin fibroblasts	557:578	mouse skin fibroblasts (NIH/3 T3)	557:589	Neither HWM nor UAM affected the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL.
29476852	4	46	theme	antioxidant	678:688	arg1	activity					690:697	in vitro antioxidant activity	669:697	in vitro antioxidant activity	669:697	UAM had a higher yield production, phenol concentration, and in vitro antioxidant activity, but it had a lower viscosity and water-holding capacity than that of HWM.
29476852	0	47	theme	functional	45:54	arg1	properties					56:65	the physicochemical and functional properties	21:65	the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	21:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	5	48	dep	Fourier	778:784	arg1	transform					786:794	transform	786:794	transform infrared spectra	786:811	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	0	49	theme	classical	138:146	arg1	extractions					156:166	ultrasonic and classical heating extractions	123:166	extractions	156:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	3	50	theme	fibroblasts	568:578	arg1	viability					544:552	the viability	540:552	the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL	540:605	Neither HWM nor UAM affected the viability of mouse skin fibroblasts (NIH/3 T3) below 100 μg/mL.
29476852	7	51	theme	various	1216:1222	arg1	extractions					1224:1234	various extractions	1216:1234	various extractions	1216:1234	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	1	52	contain	has	207:209	arg1	carpel					180:185	The cooked carpel	169:185	The cooked carpel of Nymphaea odorata	169:205	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	1	52	contain	has	207:209	arg2	mucilage					241:248	transparent mucilage	229:248	transparent mucilage	229:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	1	52	contain	has	207:209	arg2	amount					219:224	a large amount	211:224	a large amount of transparent mucilage	211:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	6	53	from	UAMD	1055:1058	arg1	polysaccharides					1084:1098	low-molecular weight polysaccharides	1063:1098	low-molecular weight polysaccharides	1063:1098	Compared to HWMD, UAMD in low-molecular weight polysaccharides increased.
29476852	8	54	theme	HWMD	1325:1328	arg1	composition					1310:1320	the main composition	1301:1320	the main composition of HWMD and UAMD	1301:1337	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	8	54	theme	HWMD	1325:1328	arg1	acid					1292:1295	glucuronic acid	1281:1295	glucuronic acid	1281:1295	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	1	55	theme	large	213:217	arg1	amount					219:224	a large amount	211:224	a large amount of transparent mucilage	211:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	1	55	theme	large	213:217	arg1	mucilage					241:248	transparent mucilage	229:248	transparent mucilage	229:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
29476852	0	56	from	carpel	90:95	arg1	properties					56:65	the physicochemical and functional properties	21:65	the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions	21:166	Comparative study on the physicochemical and functional properties of the mucilage in the carpel of Nymphaea odorata using ultrasonic and classical heating extractions.
29476852	8	57	theme	glucuronic	1281:1290	arg1	composition					1310:1320	the main composition	1301:1320	the main composition of HWMD and UAMD	1301:1337	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	8	57	theme	glucuronic	1281:1290	arg1	acid					1292:1295	glucuronic acid	1281:1295	glucuronic acid	1281:1295	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	5	58	theme	spectral	911:918	arg1	differences					920:930	major spectral differences	905:930	major spectral differences	905:930	The Fourier transform infrared spectra revealed that the dialyzed HWM and UAM, named HWMD and UAMD, respectively, appeared to have major spectral differences at 1730 cm-1 and 1605 cm-1, implying that the degree of methylation was different between HWMD and UAMD.
29476852	7	59	theme	odorata	1184:1190	arg1	carpel					1171:1176	the carpel	1167:1176	the carpel of N. odorata	1167:1190	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	2	60	theme	functional	375:384	arg1	properties					386:395	the physicochemical and functional properties	351:395	the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM)	351:508	This study compared the physicochemical and functional properties of this mucilage obtained using conventional hot water extraction (HWM) and ultrasonic-assisted extraction (UAM).
29476852	7	61	theme	basic	1123:1127	arg1	characteristics					1129:1143	the basic characteristics	1119:1143	the basic characteristics of native mucilage in the carpel of N. odorata	1119:1190	Indeed, the basic characteristics of native mucilage in the carpel of N. odorata were greatly changed by various extractions.
29476852	8	62	theme	sugar	1251:1255	arg1	analysis					1257:1264	sugar analysis	1251:1264	sugar analysis	1251:1264	Nevertheless, sugar analysis indicated that glucuronic acid was the main composition of HWMD and UAMD.
29476852	1	63	theme	transparent	229:239	arg1	mucilage					241:248	transparent mucilage	229:248	transparent mucilage	229:248	The cooked carpel of Nymphaea odorata has a large amount of transparent mucilage; however, the basic characteristics of this mucilage have not yet been reported.
31712147	4	0	theme	expression	699:708	arg1	levels					710:715	the relative mRNA and protein expression levels	669:715	levels	710:715	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	1	1	theme	present	145:151	arg1	study					153:157	The present study	141:157	The present study	141:157	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	0	2	theme	organic	74:80	arg1	acids					82:86	organic acids	74:86	organic acids	74:86	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	0	3	from	effect	11:16	arg1	metabolism					102:111	glycolipid metabolism	91:111	glycolipid metabolism	91:111	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	0	3	from	effect	11:16	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	6	4	theme	fatty	1185:1189	arg1	acids					1191:1195	short-chain fatty acids	1173:1195	short-chain fatty acids	1173:1195	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	2	5	theme	GFWE	342:345	arg1	characteristics					323:337	Structural characteristics	312:337	Structural characteristics of GFWE	312:345	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	0	6	theme	glycolipid	91:100	arg1	metabolism					102:111	glycolipid metabolism	91:111	glycolipid metabolism	91:111	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	5	7	theme	serum	1084:1088	arg1	glucose					1090:1096	serum glucose	1084:1096	serum glucose	1084:1096	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	4	8	theme	peroxisome	770:779	arg1	receptors-α					804:814	peroxisome proliferator-activated receptors-α	770:814	peroxisome proliferator-activated receptors-α	770:814	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	1	9	theme	regulatory	240:249	arg1	effects					251:257	its regulatory effects	236:257	its regulatory effects on glycolipid metabolism and gut microbiota in rats	236:309	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	3	10	theme	glucose	592:598	arg1	recovery					574:581	recovery	574:581	recovery of serum glucose and lipid	574:608	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	10	theme	glucose	592:598	arg1	reverse					615:621	reverse	615:621	reverse of liver tissue damage	615:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	10	theme	glucose	592:598	arg1	gain					568:571	weight gain	561:571	weight gain	561:571	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	1	11	from	microbiota	292:301	arg1	rats					306:309	rats	306:309	rats	306:309	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	7	12	theme	gut	1326:1328	arg1	microbiota					1330:1339	gut microbiota	1326:1339	gut microbiota	1326:1339	These results indicated that GFWE could be provided as a potential candidate to regulate glycolipid metabolism and gut microbiota.
31712147	3	13	theme	recovery	574:581	arg1	inhibition					547:556	inhibition	547:556	inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage	547:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	0	14	from	rich	46:49	arg1	polysaccharides					54:68	polysaccharides	54:68	polysaccharides	54:68	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	0	14	from	rich	46:49	arg1	acids					82:86	organic acids	74:86	organic acids	74:86	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	1	15	from	metabolism	273:282	arg1	rats					306:309	rats	306:309	rats	306:309	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	2	16	theme	liquid	430:435	arg1	chromatography					437:450	ultra-high-performance liquid chromatography	407:450	ultra-high-performance liquid chromatography	407:450	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	3	17	theme	weight	561:566	arg1	gain					568:571	weight gain	561:571	weight gain	561:571	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	6	18	theme	total	1152:1156	arg1	acids					1163:1167	total bile acids	1152:1167	total bile acids	1152:1167	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	4	19	theme	regulatory	862:871	arg1	factor-1c					903:911	sterol regulatory element-binding transcription factor-1c	855:911	sterol regulatory element-binding transcription factor-1c	855:911	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	2	20	with	chromatography	437:450	arg1	spectrometry					462:473	mass spectrometry	457:473	mass spectrometry	457:473	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	3	21	theme	gain	568:571	arg1	inhibition					547:556	inhibition	547:556	inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage	547:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	2	22	theme	liquid	381:386	arg1	chromatography					388:401	high performance liquid chromatography	364:401	high performance liquid chromatography	364:401	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	6	23	theme	acids	1163:1167	arg1	levels					1142:1147	the levels	1138:1147	the levels of total bile acids and short-chain fatty acids in the cecum	1138:1208	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	2	24	with	chromatography	388:401	arg1	spectrometry					462:473	mass spectrometry	457:473	mass spectrometry	457:473	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	2	25	theme	performance	369:379	arg1	chromatography					388:401	high performance liquid chromatography	364:401	high performance liquid chromatography	364:401	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	0	26	theme	Regulatory	0:9	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.	0:139	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	5	27	dep	abundances	970:979	arg1	abundances					970:979	the abundances	966:979	the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters	966:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	5	27	dep	abundances	970:979	arg1	Barnesiella					1018:1028	Barnesiella	1018:1028	Barnesiella	1018:1028	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	5	27	dep	abundances	970:979	arg1	Oscillibacter					1000:1012	Oscillibacter	1000:1012	Oscillibacter	1000:1012	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	4	28	theme	CoA	924:926	arg1	carboxylase					928:938	acetyl CoA carboxylase	917:938	acetyl CoA carboxylase	917:938	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	29	theme	relative	673:680	arg1	mRNA					682:685	the relative mRNA and protein expression levels	669:715	mRNA	682:685	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	30	theme	acetyl	917:922	arg1	carboxylase					928:938	acetyl CoA carboxylase	917:938	acetyl CoA carboxylase	917:938	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	2	31	theme	high	364:367	arg1	chromatography					388:401	high performance liquid chromatography	364:401	high performance liquid chromatography	364:401	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	0	32	theme	Grifola	21:27	arg1	extract					38:44	Grifola frondosa extract	21:44	Grifola frondosa extract rich in polysaccharides and organic acids	21:86	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	1	33	theme	glycolipid	262:271	arg1	metabolism					273:282	glycolipid metabolism	262:282	glycolipid metabolism	262:282	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	4	34	theme	proliferator-activated	781:802	arg1	receptors-α					804:814	peroxisome proliferator-activated receptors-α	770:814	peroxisome proliferator-activated receptors-α	770:814	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	5	35	theme	lipid	1102:1106	arg1	parameters					1108:1117	lipid parameters	1102:1117	lipid parameters	1102:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	1	36	theme	water	191:195	arg1	extract					197:203	water extract	191:203	water extract of Grifola frondosa (GFWE)	191:230	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	4	37	theme	kinase-α	760:767	arg1	levels					710:715	the relative mRNA and protein expression levels	669:715	levels	710:715	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	37	theme	kinase-α	760:767	arg1	mRNA					682:685	the relative mRNA and protein expression levels	669:715	mRNA	682:685	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	0	38	theme	gut	117:119	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	0	39	from	acids	82:86	arg1	rich					46:49	rich	46:49	rich	46:49	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	3	40	theme	serum	586:590	arg1	glucose					592:598	serum glucose	586:598	serum glucose	586:598	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	1	41	theme	extract	197:203	arg1	composition					176:186	the composition	172:186	the composition of water extract of Grifola frondosa (GFWE)	172:230	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	1	41	theme	extract	197:203	arg1	effects					251:257	its regulatory effects	236:257	its regulatory effects on glycolipid metabolism and gut microbiota in rats	236:309	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	0	42	theme	extract	38:44	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.	0:139	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	5	43	theme	bacteria	991:998	arg1	abundances					970:979	the abundances	966:979	the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters	966:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	5	43	theme	bacteria	991:998	arg1	Barnesiella					1018:1028	Barnesiella	1018:1028	Barnesiella	1018:1028	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	5	43	theme	bacteria	991:998	arg1	Oscillibacter					1000:1012	Oscillibacter	1000:1012	Oscillibacter	1000:1012	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	4	44	theme	element-binding	873:887	arg1	factor-1c					903:911	sterol regulatory element-binding transcription factor-1c	855:911	sterol regulatory element-binding transcription factor-1c	855:911	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	2	45	theme	ultra-high-performance	407:428	arg1	chromatography					437:450	ultra-high-performance liquid chromatography	407:450	ultra-high-performance liquid chromatography	407:450	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	0	46	theme	frondosa	29:36	arg1	extract					38:44	Grifola frondosa extract	21:44	Grifola frondosa extract rich in polysaccharides and organic acids	21:86	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	1	47	theme	gut	288:290	arg1	microbiota					292:301	gut microbiota	288:301	gut microbiota	288:301	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	5	48	with	correlation	1067:1077	arg1	glucose					1090:1096	serum glucose	1084:1096	serum glucose	1084:1096	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	5	48	with	correlation	1067:1077	arg1	parameters					1108:1117	lipid parameters	1102:1117	lipid parameters	1102:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	4	49	theme	receptors-α	804:814	arg1	levels					710:715	the relative mRNA and protein expression levels	669:715	levels	710:715	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	49	theme	receptors-α	804:814	arg1	mRNA					682:685	the relative mRNA and protein expression levels	669:715	mRNA	682:685	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	2	50	theme	mass	457:460	arg1	spectrometry					462:473	mass spectrometry	457:473	mass spectrometry	457:473	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	3	51	theme	hypoglycemic	480:491	arg1	activities					511:520	The hypoglycemic and hypolipidemic activities	476:520	The hypoglycemic and hypolipidemic activities of GFWE	476:528	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	7	52	theme	potential	1268:1276	arg1	candidate					1278:1286	a potential candidate	1266:1286	a potential candidate to regulate glycolipid metabolism and gut microbiota	1266:1339	These results indicated that GFWE could be provided as a potential candidate to regulate glycolipid metabolism and gut microbiota.
31712147	7	52	theme	potential	1268:1276	arg1	GFWE					1240:1243	GFWE	1240:1243	GFWE	1240:1243	These results indicated that GFWE could be provided as a potential candidate to regulate glycolipid metabolism and gut microbiota.
31712147	0	53	from	polysaccharides	54:68	arg1	rich					46:49	rich	46:49	rich	46:49	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	3	54	theme	lipid	604:608	arg1	recovery					574:581	recovery	574:581	recovery of serum glucose and lipid	574:608	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	54	theme	lipid	604:608	arg1	reverse					615:621	reverse	615:621	reverse of liver tissue damage	615:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	54	theme	lipid	604:608	arg1	gain					568:571	weight gain	561:571	weight gain	561:571	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	6	55	theme	acids	1191:1195	arg1	levels					1142:1147	the levels	1138:1147	the levels of total bile acids and short-chain fatty acids in the cecum	1138:1208	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	3	56	theme	GFWE	525:528	arg1	activities					511:520	The hypoglycemic and hypolipidemic activities	476:520	The hypoglycemic and hypolipidemic activities of GFWE	476:528	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	0	57	theme	rich	46:49	arg1	extract					38:44	Grifola frondosa extract	21:44	Grifola frondosa extract rich in polysaccharides and organic acids	21:86	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	6	58	theme	short-chain	1173:1183	arg1	acids					1191:1195	short-chain fatty acids	1173:1195	short-chain fatty acids	1173:1195	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	4	59	theme	transcription	889:901	arg1	factor-1c					903:911	sterol regulatory element-binding transcription factor-1c	855:911	sterol regulatory element-binding transcription factor-1c	855:911	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	60	theme	adenine	720:726	arg1	kinase-α					760:767	adenine monophosphate activated protein kinase-α	720:767	adenine monophosphate activated protein kinase-α	720:767	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	61	theme	monophosphate	728:740	arg1	kinase-α					760:767	adenine monophosphate activated protein kinase-α	720:767	adenine monophosphate activated protein kinase-α	720:767	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	0	62	from	metabolism	102:111	arg1	rats					135:138	rats	135:138	rats	135:138	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	3	63	theme	tissue	632:637	arg1	damage					639:644	liver tissue damage	626:644	liver tissue damage	626:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	64	theme	reverse	615:621	arg1	inhibition					547:556	inhibition	547:556	inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage	547:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	2	65	theme	Structural	312:321	arg1	characteristics					323:337	Structural characteristics	312:337	Structural characteristics of GFWE	312:345	Structural characteristics of GFWE were detected by high performance liquid chromatography and ultra-high-performance liquid chromatography with mass spectrometry.
31712147	1	66	theme	frondosa	216:223	arg1	extract					197:203	water extract	191:203	water extract of Grifola frondosa (GFWE)	191:230	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	4	67	theme	glucokinase	821:831	arg1	levels					710:715	the relative mRNA and protein expression levels	669:715	levels	710:715	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	4	67	theme	glucokinase	821:831	arg1	mRNA					682:685	the relative mRNA and protein expression levels	669:715	mRNA	682:685	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	5	68	theme	caecal	984:989	arg1	bacteria					991:998	caecal bacteria	984:998	caecal bacteria	984:998	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	3	69	theme	damage	639:644	arg1	recovery					574:581	recovery	574:581	recovery of serum glucose and lipid	574:608	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	69	theme	damage	639:644	arg1	reverse					615:621	reverse	615:621	reverse of liver tissue damage	615:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	3	69	theme	damage	639:644	arg1	gain					568:571	weight gain	561:571	weight gain	561:571	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	1	70	from	composition	176:186	arg1	metabolism					273:282	glycolipid metabolism	262:282	glycolipid metabolism	262:282	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	1	70	from	composition	176:186	arg1	microbiota					292:301	gut microbiota	288:301	gut microbiota	288:301	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	4	71	theme	activated	742:750	arg1	kinase-α					760:767	adenine monophosphate activated protein kinase-α	720:767	adenine monophosphate activated protein kinase-α	720:767	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	3	72	theme	hypolipidemic	497:509	arg1	activities					511:520	The hypoglycemic and hypolipidemic activities	476:520	The hypoglycemic and hypolipidemic activities of GFWE	476:528	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	4	73	theme	protein	752:758	arg1	kinase-α					760:767	adenine monophosphate activated protein kinase-α	720:767	adenine monophosphate activated protein kinase-α	720:767	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	7	74	theme	glycolipid	1300:1309	arg1	metabolism					1311:1320	glycolipid metabolism	1300:1320	glycolipid metabolism	1300:1320	These results indicated that GFWE could be provided as a potential candidate to regulate glycolipid metabolism and gut microbiota.
31712147	5	75	theme	negative	1058:1065	arg1	correlation					1067:1077	a significant negative correlation	1044:1077	a significant negative correlation with serum glucose and lipid parameters	1044:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	4	76	theme	sterol	855:860	arg1	factor-1c					903:911	sterol regulatory element-binding transcription factor-1c	855:911	sterol regulatory element-binding transcription factor-1c	855:911	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	1	77	from	effects	251:257	arg1	metabolism					273:282	glycolipid metabolism	262:282	glycolipid metabolism	262:282	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	1	77	from	effects	251:257	arg1	microbiota					292:301	gut microbiota	288:301	gut microbiota	288:301	The present study investigated the composition of water extract of Grifola frondosa (GFWE) and its regulatory effects on glycolipid metabolism and gut microbiota in rats.
31712147	6	78	theme	bile	1158:1161	arg1	acids					1163:1167	total bile acids	1152:1167	total bile acids	1152:1167	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31712147	0	79	from	microbiota	121:130	arg1	rats					135:138	rats	135:138	rats	135:138	Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats.
31712147	5	80	theme	significant	1046:1056	arg1	correlation					1067:1077	a significant negative correlation	1044:1077	a significant negative correlation with serum glucose and lipid parameters	1044:1117	Moreover, GFWE increased the abundances of caecal bacteria Oscillibacter and Barnesiella, which showed a significant negative correlation with serum glucose and lipid parameters.
31712147	3	81	theme	liver	626:630	arg1	damage					639:644	liver tissue damage	626:644	liver tissue damage	626:644	The hypoglycemic and hypolipidemic activities of GFWE were obtained by inhibition of weight gain, recovery of serum glucose and lipid, and reverse of liver tissue damage.
31712147	4	82	theme	protein	691:697	arg1	levels					710:715	the relative mRNA and protein expression levels	669:715	levels	710:715	GFWE has up-regulated the relative mRNA and protein expression levels of adenine monophosphate activated protein kinase-α, peroxisome proliferator-activated receptors-α, and glucokinase, while down-regulated sterol regulatory element-binding transcription factor-1c and acetyl CoA carboxylase.
31712147	6	83	from	levels	1142:1147	arg1	cecum					1204:1208	the cecum	1200:1208	the cecum	1200:1208	And GFWE enhanced the levels of total bile acids and short-chain fatty acids in the cecum.
31362137	4	0	theme	%	665:665	arg1	DE					657:658	a DE	655:658	a DE of 76% mixed with arabinogalactan	655:692	Highly methyl esterified homogalacturonan with a DE of 76% mixed with arabinogalactan were found.
31362137	6	1	theme	apple	936:940	arg1	pectins					942:948	cashew apple pectins	929:948	cashew apple pectins	929:948	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	1	2	theme	nut	308:310	arg1	production					312:321	the nut production	304:321	the nut production	304:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	6	3	theme	industrial	1049:1058	arg1	production					1086:1095	this industrial waste from the cashew nut production	1044:1095	this industrial waste from the cashew nut production	1044:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	6	4	theme	cashew	929:934	arg1	pectins					942:948	cashew apple pectins	929:948	cashew apple pectins	929:948	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	1	5	theme	production	312:321	arg1	waste					295:299	an industrial waste	281:299	an industrial waste of the nut production	281:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	5	6	theme	rhamnogalacturonan	825:842	arg1	arabinogalactan					771:785	a highly branched type II arabinogalactan	745:785	a highly branched type II arabinogalactan (AG II)	745:793	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	6	theme	rhamnogalacturonan	825:842	arg1	rhamnogalacturonan					825:842	a type I rhamnogalacturonan	816:842	a type I rhamnogalacturonan in which the AG II could be anchored	816:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	6	theme	rhamnogalacturonan	825:842	arg1	amounts					805:811	small amounts	799:811	small amounts of a type I rhamnogalacturonan in which the AG II could be anchored	799:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	6	theme	rhamnogalacturonan	825:842	arg1	AG					788:789	AG II	788:792	AG II	788:792	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	0	7	theme	chemical	73:80	arg1	characterization					82:97	chemical characterization	73:97	chemical characterization	73:97	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	7	theme	chemical	73:80	arg1	Pectins					0:6	Pectins	0:6	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.	0:98	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	6	8	theme	pectins	942:948	arg1	structure					916:924	the chemical structure	903:924	the chemical structure of cashew apple pectins	903:948	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	6	9	theme	nut	1082:1084	arg1	production					1086:1095	this industrial waste from the cashew nut production	1044:1095	this industrial waste from the cashew nut production	1044:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	1	10	from	carbohydrates	252:264	arg1	rich					244:247	rich	244:247	rich	244:247	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	2	11	located	found	422:426	arg1	apple					438:442	cashew apple	431:442	cashew apple	431:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	2	11	located	found	422:426	arg2	polysaccharides					406:420	cell wall polysaccharides	396:420	cell wall polysaccharides found in cashew apple	396:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	1	12	theme	tropical	146:153	arg1	tree					165:168	a tropical evergreen tree	144:168	a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production	144:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	12	theme	tropical	146:153	arg1	tree					111:114	The cashew tree	100:114	The cashew tree (Anacardium occidentale)	100:139	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	2	13	theme	polysaccharides	406:420	arg1	structure					383:391	the chemical structure	370:391	the chemical structure of cell wall polysaccharides found in cashew apple	370:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	5	14	theme	II	768:769	arg1	arabinogalactan					771:785	a highly branched type II arabinogalactan	745:785	a highly branched type II arabinogalactan (AG II)	745:793	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	14	theme	II	768:769	arg1	AG					788:789	AG II	788:792	AG II	788:792	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	6	15	dep	nutritional	985:995	arg1	uses					1035:1038	uses	1035:1038	uses for this industrial waste from the cashew nut production	1035:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	1	16	theme	evergreen	155:163	arg1	tree					165:168	a tropical evergreen tree	144:168	a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production	144:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	16	theme	evergreen	155:163	arg1	tree					111:114	The cashew tree	100:114	The cashew tree (Anacardium occidentale)	100:139	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	2	17	theme	wall	401:404	arg1	polysaccharides					406:420	cell wall polysaccharides	396:420	cell wall polysaccharides found in cashew apple	396:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	0	18	from	fruit	26:30	arg1	Extraction					58:67	Extraction	58:67	Extraction	58:67	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	18	from	fruit	26:30	arg1	characterization					82:97	chemical characterization	73:97	chemical characterization	73:97	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	18	from	fruit	26:30	arg1	Pectins					0:6	Pectins	0:6	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.	0:98	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	1	19	from	rich	244:247	arg1	carbohydrates					252:264	carbohydrates	252:264	carbohydrates	252:264	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	6	20	theme	chemical	907:914	arg1	structure					916:924	the chemical structure	903:924	the chemical structure of cashew apple pectins	903:948	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	2	21	theme	cell	396:399	arg1	polysaccharides					406:420	cell wall polysaccharides	396:420	cell wall polysaccharides found in cashew apple	396:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	1	22	theme	rich	244:247	arg1	apple					226:230	the cashew apple	215:230	the cashew apple	215:230	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	22	theme	rich	244:247	arg1	peduncle					235:242	a peduncle	233:242	a peduncle rich in carbohydrates	233:264	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	5	23	theme	type	818:821	arg1	rhamnogalacturonan					825:842	a type I rhamnogalacturonan	816:842	a type I rhamnogalacturonan in which the AG II could be anchored	816:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	0	24	theme	apple	20:24	arg1	fruit					26:30	cashew apple fruit	13:30	cashew apple fruit (Anacardium occidentale)	13:55	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	24	theme	apple	20:24	arg1	occidentale					44:54	Anacardium occidentale	33:54	Anacardium occidentale	33:54	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	2	25	theme	chemical	374:381	arg1	structure					383:391	the chemical structure	370:391	the chemical structure of cell wall polysaccharides found in cashew apple	370:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	0	26	theme	cashew	13:18	arg1	fruit					26:30	cashew apple fruit	13:30	cashew apple fruit (Anacardium occidentale)	13:55	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	26	theme	cashew	13:18	arg1	occidentale					44:54	Anacardium occidentale	33:54	Anacardium occidentale	33:54	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	3	27	theme	monosaccharide	518:531	arg1	composition					533:543	monosaccharide composition	518:543	monosaccharide composition	518:543	In this work, its pectic polysaccharides have been characterized through monosaccharide composition, HPSEC, methylation and 13C and 1H/13C HSQC-DEPT-NMR analyses.
31362137	6	28	theme	waste	1060:1064	arg1	production					1086:1095	this industrial waste from the cashew nut production	1044:1095	this industrial waste from the cashew nut production	1044:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	2	29	theme	cashew	431:436	arg1	apple					438:442	cashew apple	431:442	cashew apple	431:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	1	30	dep	tree	165:168	arg1	considered					270:279	considered	270:279	considered an industrial waste of the nut production	270:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	30	dep	tree	165:168	arg1	cultivated					178:187	cultivated	178:187	largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates	170:264	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	4	31	with	homogalacturonan	633:648	arg1	DE					657:658	a DE	655:658	a DE of 76% mixed with arabinogalactan	655:692	Highly methyl esterified homogalacturonan with a DE of 76% mixed with arabinogalactan were found.
31362137	2	32	theme	available	354:362	arg1	data					349:352	no data	346:352	no data available about the chemical structure of cell wall polysaccharides found in cashew apple	346:442	Until now, there were no data available about the chemical structure of cell wall polysaccharides found in cashew apple.
31362137	5	33	theme	branched	754:761	arg1	arabinogalactan					771:785	a highly branched type II arabinogalactan	745:785	a highly branched type II arabinogalactan (AG II)	745:793	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	33	theme	branched	754:761	arg1	AG					788:789	AG II	788:792	AG II	788:792	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	34	theme	type	763:766	arg1	arabinogalactan					771:785	a highly branched type II arabinogalactan	745:785	a highly branched type II arabinogalactan (AG II)	745:793	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	34	theme	type	763:766	arg1	AG					788:789	AG II	788:792	AG II	788:792	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	1	35	theme	cashew	104:109	arg1	tree					165:168	a tropical evergreen tree	144:168	a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production	144:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	35	theme	cashew	104:109	arg1	tree					111:114	The cashew tree	100:114	The cashew tree (Anacardium occidentale)	100:139	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	35	theme	cashew	104:109	arg1	occidentale					128:138	Anacardium occidentale	117:138	Anacardium occidentale	117:138	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	0	36	theme	Anacardium	33:42	arg1	fruit					26:30	cashew apple fruit	13:30	cashew apple fruit (Anacardium occidentale)	13:55	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	36	theme	Anacardium	33:42	arg1	occidentale					44:54	Anacardium occidentale	33:54	Anacardium occidentale	33:54	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	1	37	theme	industrial	284:293	arg1	waste					295:299	an industrial waste	281:299	an industrial waste of the nut production	281:321	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	6	38	theme	cashew	1075:1080	arg1	production					1086:1095	this industrial waste from the cashew nut production	1044:1095	this industrial waste from the cashew nut production	1044:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	4	39	dep	esterified	622:631	arg1	methyl					615:620	methyl	615:620	methyl	615:620	Highly methyl esterified homogalacturonan with a DE of 76% mixed with arabinogalactan were found.
31362137	3	40	theme	1H/13C	577:582	arg1	analyses					598:605	1H/13C HSQC-DEPT-NMR analyses	577:605	1H/13C HSQC-DEPT-NMR analyses	577:605	In this work, its pectic polysaccharides have been characterized through monosaccharide composition, HPSEC, methylation and 13C and 1H/13C HSQC-DEPT-NMR analyses.
31362137	6	41	theme	from	1066:1069	arg1	production					1086:1095	this industrial waste from the cashew nut production	1044:1095	this industrial waste from the cashew nut production	1044:1095	These findings about the chemical structure of cashew apple pectins could contribute to develop future nutritional, biotechnological and pharmacological uses for this industrial waste from the cashew nut production.
31362137	3	42	theme	HSQC-DEPT-NMR	584:596	arg1	analyses					598:605	1H/13C HSQC-DEPT-NMR analyses	577:605	1H/13C HSQC-DEPT-NMR analyses	577:605	In this work, its pectic polysaccharides have been characterized through monosaccharide composition, HPSEC, methylation and 13C and 1H/13C HSQC-DEPT-NMR analyses.
31362137	1	43	theme	Anacardium	117:126	arg1	tree					111:114	The cashew tree	100:114	The cashew tree (Anacardium occidentale)	100:139	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	43	theme	Anacardium	117:126	arg1	occidentale					128:138	Anacardium occidentale	117:138	Anacardium occidentale	117:138	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	5	44	theme	small	799:803	arg1	rhamnogalacturonan					825:842	a type I rhamnogalacturonan	816:842	a type I rhamnogalacturonan in which the AG II could be anchored	816:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	44	theme	small	799:803	arg1	amounts					805:811	small amounts	799:811	small amounts of a type I rhamnogalacturonan in which the AG II could be anchored	799:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	5	45	theme	I	823:823	arg1	rhamnogalacturonan					825:842	a type I rhamnogalacturonan	816:842	a type I rhamnogalacturonan in which the AG II could be anchored	816:879	This latter was purified and presented a highly branched type II arabinogalactan (AG II) and small amounts of a type I rhamnogalacturonan in which the AG II could be anchored.
31362137	4	46	theme	esterified	622:631	arg1	homogalacturonan					633:648	Highly methyl esterified homogalacturonan	608:648	Highly methyl esterified homogalacturonan with a DE of 76% mixed with arabinogalactan	608:692	Highly methyl esterified homogalacturonan with a DE of 76% mixed with arabinogalactan were found.
31362137	0	47	dep	Pectins	0:6	arg1	Extraction					58:67	Extraction	58:67	Extraction	58:67	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	47	dep	Pectins	0:6	arg1	characterization					82:97	chemical characterization	73:97	chemical characterization	73:97	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	0	47	dep	Pectins	0:6	arg1	Pectins					0:6	Pectins	0:6	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.	0:98	Pectins from cashew apple fruit (Anacardium occidentale): Extraction and chemical characterization.
31362137	3	48	theme	pectic	463:468	arg1	polysaccharides					470:484	its pectic polysaccharides	459:484	its pectic polysaccharides	459:484	In this work, its pectic polysaccharides have been characterized through monosaccharide composition, HPSEC, methylation and 13C and 1H/13C HSQC-DEPT-NMR analyses.
31362137	1	49	theme	cashew	219:224	arg1	apple					226:230	the cashew apple	215:230	the cashew apple	215:230	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31362137	1	49	theme	cashew	219:224	arg1	peduncle					235:242	a peduncle	233:242	a peduncle rich in carbohydrates	233:264	The cashew tree (Anacardium occidentale) is a tropical evergreen tree largely cultivated in Brazil, which produces the cashew apple, a peduncle rich in carbohydrates and considered an industrial waste of the nut production.
31494158	9	0	with	systems	1440:1446	arg1	properties					1470:1479	improved thermal properties	1453:1479	improved thermal properties	1453:1479	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	3	1	theme	compatibility	594:606	arg1	level					585:589	a lower level	577:589	a lower level of compatibility	577:606	However, higher inclusion of POSS results in the formation of aggregates and thus a lower level of compatibility.
31494158	4	2	located	localized	735:743	arg2	POSS					717:720	POSS	717:720	POSS	717:720	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	4	2	located	localized	735:743	arg1	phases					768:773	the PLA and PCL phases	752:773	the PLA and PCL phases	752:773	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	1	3	theme	oligomeric	201:210	arg1	nanoparticles					234:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	2	4	theme	lower	414:418	arg1	%					450:450	0.5 and 1 wt%	438:450	0.5 and 1 wt%	438:450	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	2	4	theme	lower	414:418	arg1	contents					420:427	lower contents	414:427	lower contents of POSS (0.5 and 1 wt%)	414:451	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	1	5	theme	hydrophobic	305:315	arg1	poly					317:320	hydrophobic poly	305:320	hydrophobic poly(lactic acid) (PLA)	305:339	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	5	theme	hydrophobic	305:315	arg1	acid					329:332	lactic acid	322:332	lactic acid	322:332	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	5	theme	hydrophobic	305:315	arg1	PLA					336:338	PLA	336:338	PLA	336:338	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	4	6	theme	PLA	756:758	arg1	phases					768:773	the PLA and PCL phases	752:773	the PLA and PCL phases	752:773	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	5	7	theme	differential	785:796	arg1	calorimetry					807:817	differential scanning calorimetry	785:817	differential scanning calorimetry	785:817	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	1	8	theme	silsesquioxane	212:225	arg1	nanoparticles					234:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	5	9	theme	22.9	855:858	arg1	%					859:859	%	859:859	%	859:859	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	8	10	theme	good	1293:1296	arg1	dispersion					1303:1312	good POSS dispersion	1293:1312	good POSS dispersion	1293:1312	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	0	11	from	Study	121:125	arg1	properties					138:147	thermal properties	130:147	thermal properties	130:147	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	7	12	theme	crystallization	1039:1053	arg1	behavior					1055:1062	the crystallization behavior	1035:1062	the crystallization behavior	1035:1062	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	3	13	theme	lower	579:583	arg1	level					585:589	a lower level	577:589	a lower level of compatibility	577:606	However, higher inclusion of POSS results in the formation of aggregates and thus a lower level of compatibility.
31494158	4	14	theme	PCL	764:766	arg1	phases					768:773	the PLA and PCL phases	752:773	the PLA and PCL phases	752:773	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	1	15	theme	POSS	228:231	arg1	nanoparticles					234:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	16	theme	lactic	322:327	arg1	poly					317:320	hydrophobic poly	305:320	hydrophobic poly(lactic acid) (PLA)	305:339	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	16	theme	lactic	322:327	arg1	acid					329:332	lactic acid	322:332	lactic acid	322:332	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	PCL					367:369	PCL	367:369	PCL	367:369	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	acid					329:332	lactic acid	322:332	lactic acid	322:332	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	poly					345:348	poly	345:348	poly(ɛ-caprolactone) (PCL)	345:370	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	ɛ-caprolactone					350:363	ɛ-caprolactone	350:363	ɛ-caprolactone	350:363	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	poly					317:320	hydrophobic poly	305:320	hydrophobic poly(lactic acid) (PLA)	305:339	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	1	17	with	compatibility	264:276	arg1	PLA					336:338	PLA	336:338	PLA	336:338	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	6	18	theme	crystallites	1004:1015	arg1	formation					981:989	formation	981:989	formation of these PLA crystallites	981:1015	However, the PCL's crystallinity is slightly hampered due to formation of these PLA crystallites.
31494158	7	19	used	utilized	1232:1239	arg2	%					1217:1217	3 and 5 wt%	1207:1217	3 and 5 wt%	1207:1217	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	7	19	used	utilized	1232:1239	arg2	contents					1197:1204	moderate to high contents	1180:1204	moderate to high contents (3 and 5 wt%) of POSS	1180:1226	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	5	20	theme	31.6	864:867	arg1	%					859:859	%	859:859	%	859:859	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	8	21	theme	lower	1347:1351	arg1	contents					1353:1360	lower contents	1347:1360	lower contents	1347:1360	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	0	22	theme	Enhanced	0:7	arg1	compatibility					9:21	Enhanced compatibility	0:21	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.	0:148	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	7	23	theme	POSS	1223:1226	arg1	%					1217:1217	3 and 5 wt%	1207:1217	3 and 5 wt%	1207:1217	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	7	23	theme	POSS	1223:1226	arg1	contents					1197:1204	moderate to high contents	1180:1204	moderate to high contents (3 and 5 wt%) of POSS	1180:1226	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	0	24	theme	starch	26:31	arg1	compatibility					9:21	Enhanced compatibility	0:21	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.	0:148	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	1	25	theme	nanoparticles	234:246	arg1	ability					179:185	the ability	175:185	the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL)	175:370	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	5	26	theme	scanning	798:805	arg1	calorimetry					807:817	differential scanning calorimetry	785:817	differential scanning calorimetry	785:817	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	9	27	theme	efficient	1396:1404	arg1	compatibilizer					1406:1419	an efficient compatibilizer	1393:1419	an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties	1393:1479	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	9	27	theme	efficient	1396:1404	arg1	POSS					1378:1381	POSS	1378:1381	POSS	1378:1381	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	7	28	theme	thermal	1136:1142	arg1	stability					1144:1152	the composite's thermal stability	1120:1152	the composite's thermal stability	1120:1152	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	8	29	theme	analysis	1261:1268	arg1	results					1270:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	7	30	theme	thermal	1081:1087	arg1	kinetics					1101:1108	the thermal degradation kinetics	1077:1108	the thermal degradation kinetics	1077:1108	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	8	31	theme	Dynamic	1242:1248	arg1	results					1270:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	0	32	theme	nanoparticles	106:118	arg1	incorporation					84:96	incorporation	84:96	incorporation of POSS nanoparticles	84:118	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	8	33	theme	mechanical	1250:1259	arg1	results					1270:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results	1242:1276	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	7	34	with	contrast	1021:1028	arg1	behavior					1055:1062	the crystallization behavior	1035:1062	the crystallization behavior	1035:1062	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	7	35	dep	high	1192:1195	arg1	to					1189:1190	to	1189:1190	to	1189:1190	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	9	36	theme	improved	1453:1460	arg1	properties					1470:1479	improved thermal properties	1453:1479	improved thermal properties	1453:1479	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	0	37	theme	POSS	101:104	arg1	nanoparticles					106:118	POSS nanoparticles	101:118	POSS nanoparticles	101:118	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	3	38	theme	higher	504:509	arg1	inclusion					511:519	higher inclusion	504:519	higher inclusion of POSS	504:527	However, higher inclusion of POSS results in the formation of aggregates and thus a lower level of compatibility.
31494158	0	39	with	starch	26:31	arg1	poly					38:41	poly	38:41	poly(lactic acid)	38:54	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	0	39	with	starch	26:31	arg1	acid					50:53	lactic acid	43:53	lactic acid	43:53	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	0	39	with	starch	26:31	arg1	poly					60:63	poly	60:63	poly(ɛ-caprolactone)	60:79	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	0	39	with	starch	26:31	arg1	ɛ-caprolactone					65:78	ɛ-caprolactone	65:78	ɛ-caprolactone	65:78	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	5	40	dep	%	859:859	arg1	to					861:862	to	861:862	to	861:862	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	0	41	theme	lactic	43:48	arg1	poly					38:41	poly	38:41	poly(lactic acid)	38:54	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	0	41	theme	lactic	43:48	arg1	acid					50:53	lactic acid	43:53	lactic acid	43:53	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	2	42	theme	system	487:492	arg1	compatibility					466:478	the compatibility	462:478	the compatibility of the system	462:492	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	4	43	theme	Transmission	609:620	arg1	microscopy					631:640	Transmission electron microscopy	609:640	Transmission electron microscopy	609:640	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	2	44	theme	Morphological	373:385	arg1	analysis					387:394	Morphological analysis	373:394	Morphological analysis	373:394	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	5	45	theme	POSS	904:907	arg1	%					916:916	0.5 wt%	910:916	0.5 wt%	910:916	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	5	45	theme	POSS	904:907	arg1	content					893:899	a very low content	882:899	a very low content of POSS (0.5 wt%)	882:917	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	9	46	theme	PLA/starch/PCL	1425:1438	arg1	systems					1440:1446	PLA/starch/PCL systems	1425:1446	PLA/starch/PCL systems with improved thermal properties	1425:1479	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	1	47	theme	hydrophilic	281:291	arg1	starch					293:298	hydrophilic starch	281:298	hydrophilic starch	281:298	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	0	48	theme	thermal	130:136	arg1	properties					138:147	thermal properties	130:147	thermal properties	130:147	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	7	49	theme	high	1192:1195	arg1	%					1217:1217	3 and 5 wt%	1207:1217	3 and 5 wt%	1207:1217	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	7	49	theme	high	1192:1195	arg1	contents					1197:1204	moderate to high contents	1180:1204	moderate to high contents (3 and 5 wt%) of POSS	1180:1226	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	3	50	theme	POSS	524:527	arg1	inclusion					511:519	higher inclusion	504:519	higher inclusion of POSS	504:527	However, higher inclusion of POSS results in the formation of aggregates and thus a lower level of compatibility.
31494158	5	51	theme	low	889:891	arg1	%					916:916	0.5 wt%	910:916	0.5 wt%	910:916	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	5	51	theme	low	889:891	arg1	content					893:899	a very low content	882:899	a very low content of POSS (0.5 wt%)	882:917	Based on differential scanning calorimetry, PLA's crystallinity increases from 22.9% to 31.6% upon adding a very low content of POSS (0.5 wt%).
31494158	6	52	theme	PLA	1000:1002	arg1	crystallites					1004:1015	these PLA crystallites	994:1015	these PLA crystallites	994:1015	However, the PCL's crystallinity is slightly hampered due to formation of these PLA crystallites.
31494158	3	53	theme	aggregates	557:566	arg1	formation					544:552	the formation	540:552	the formation of aggregates	540:566	However, higher inclusion of POSS results in the formation of aggregates and thus a lower level of compatibility.
31494158	4	54	theme	electron	622:629	arg1	microscopy					631:640	Transmission electron microscopy	609:640	Transmission electron microscopy	609:640	Transmission electron microscopy revealed that PCL acts as an intermediate between PLA and starch, and that POSS is primarily localized within the PLA and PCL phases.
31494158	2	55	theme	POSS	432:435	arg1	%					450:450	0.5 and 1 wt%	438:450	0.5 and 1 wt%	438:450	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	2	55	theme	POSS	432:435	arg1	contents					420:427	lower contents	414:427	lower contents of POSS (0.5 and 1 wt%)	414:451	Morphological analysis demonstrated that lower contents of POSS (0.5 and 1 wt%) enhances the compatibility of the system.
31494158	8	56	theme	POSS	1298:1301	arg1	dispersion					1303:1312	good POSS dispersion	1293:1312	good POSS dispersion	1293:1312	Dynamic mechanical analysis results also confirmed good POSS dispersion within the matrix, especially at lower contents.
31494158	0	57	dep	compatibility	9:21	arg1	Study					121:125	Study	121:125	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.	0:148	Enhanced compatibility of starch with poly(lactic acid) and poly(ɛ-caprolactone) by incorporation of POSS nanoparticles: Study on thermal properties.
31494158	7	58	theme	degradation	1089:1099	arg1	kinetics					1101:1108	the thermal degradation kinetics	1077:1108	the thermal degradation kinetics	1077:1108	In contrast with the crystallization behavior and based on the thermal degradation kinetics, we found the composite's thermal stability is greatly increased when moderate to high contents (3 and 5 wt%) of POSS are utilized.
31494158	1	59	theme	starch	293:298	arg1	compatibility					264:276	the compatibility	260:276	the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL)	260:370	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31494158	9	60	theme	thermal	1462:1468	arg1	properties					1470:1479	improved thermal properties	1453:1479	improved thermal properties	1453:1479	In conclusion, POSS serves as an efficient compatibilizer for PLA/starch/PCL systems with improved thermal properties.
31494158	1	61	theme	polyhedral	190:199	arg1	nanoparticles					234:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	polyhedral oligomeric silsesquioxane (POSS) nanoparticles	190:246	In this work, we explore the ability of polyhedral oligomeric silsesquioxane (POSS) nanoparticles to increase the compatibility of hydrophilic starch with hydrophobic poly(lactic acid) (PLA) and poly(ɛ-caprolactone) (PCL).
31108145	2	0	theme	high	474:477	arg1	HPLC					514:517	HPLC	514:517	HPLC	514:517	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	0	theme	high	474:477	arg1	chromatography					498:511	high performance liquid chromatography	474:511	high performance liquid chromatography (HPLC)	474:518	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	6	1	theme	Western	1296:1302	arg1	analysis					1309:1316	Western blot analysis	1296:1316	Western blot analysis	1296:1316	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	2	theme	blot	1304:1307	arg1	analysis					1309:1316	Western blot analysis	1296:1316	Western blot analysis	1296:1316	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	2	3	theme	composition	358:368	arg1	Analysis					331:338	Analysis	331:338	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc)	331:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	1	4	theme	H2O2-induced	238:249	arg1	damage					261:266	H2O2-induced oxidative damage	238:266	H2O2-induced oxidative damage in IPEC-1 cells	238:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	0	5	theme	oxidative	93:101	arg1	stress					103:108	hydrogen peroxide-induced oxidative stress	67:108	hydrogen peroxide-induced oxidative stress	67:108	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	8	6	theme	pathway	1537:1543	arg1	activation					1498:1507	activation	1498:1507	activation of the Keap1/Nrf2 signaling pathway	1498:1543	This protection mechanism may be related to activation of the Keap1/Nrf2 signaling pathway.
31108145	1	7	theme	Hemp	169:172	arg1	HSP					195:197	HSP	195:197	HSP	195:197	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	7	theme	Hemp	169:172	arg1	polysaccharide					179:192	Hemp seed polysaccharide	169:192	Hemp seed polysaccharide (HSP)	169:198	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	8	theme	oxidative	251:259	arg1	damage					261:266	H2O2-induced oxidative damage	238:266	H2O2-induced oxidative damage in IPEC-1 cells	238:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	2	9	dep	Fourier	524:530	arg1	transform					532:540	transform	532:540	transform infrared spectroscopy (FT-IR)	532:570	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	7	10	theme	present	1337:1343	arg1	study					1345:1349	the present study	1333:1349	the present study	1333:1349	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	5	11	theme	fluorescent	993:1003	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	11	theme	fluorescent	993:1003	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	12	theme	reverse	1018:1024	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	12	theme	reverse	1018:1024	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	7	13	contain	has	1373:1375	arg1	HSP0.2					1366:1371	HSP0.2	1366:1371	HSP0.2	1366:1371	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	7	13	contain	has	1373:1375	arg2	effect					1392:1397	the protective effect	1377:1397	the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress	1377:1451	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	4	14	theme	LDH	716:718	arg1	release					705:711	the release	701:711	the release of LDH and MDA	701:726	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	6	15	theme	Kelch-like	1247:1256	arg1	protein					1273:1279	Kelch-like ECH-associated protein 1	1247:1281	Kelch-like ECH-associated protein 1 (Keap1)	1247:1289	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	15	theme	Kelch-like	1247:1256	arg1	Keap1					1284:1288	Keap1	1284:1288	Keap1	1284:1288	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	1	16	from	mechanism	301:309	arg1	damage					261:266	H2O2-induced oxidative damage	238:266	H2O2-induced oxidative damage in IPEC-1 cells	238:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	4	17	theme	MDA	724:726	arg1	release					705:711	the release	701:711	the release of LDH and MDA	701:726	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	6	18	theme	expression	1114:1123	arg1	level					1125:1129	the expression level	1110:1129	the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1110:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	4	19	theme	CAT	795:797	arg1	activities					765:774	the activities	761:774	the activities of SOD, GSH-Px and CAT	761:797	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	1	20	from	structure	156:164	arg1	damage					261:266	H2O2-induced oxidative damage	238:266	H2O2-induced oxidative damage in IPEC-1 cells	238:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	6	21	theme	factor	1204:1209	arg1	level					1125:1129	the expression level	1110:1129	the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1110:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	1	22	theme	seed	174:177	arg1	HSP					195:197	HSP	195:197	HSP	195:197	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	22	theme	seed	174:177	arg1	polysaccharide					179:192	Hemp seed polysaccharide	169:192	Hemp seed polysaccharide (HSP)	169:198	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	2	23	dep	transform	532:540	arg1	infrared					542:549	infrared	542:549	transform infrared spectroscopy (FT-IR)	532:570	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	24	from	composition	358:368	arg1	polysaccharide					422:435	polysaccharide	422:435	polysaccharide of Hemp seed (HSPc)	422:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	25	theme	seed	445:448	arg1	polysaccharide					422:435	polysaccharide	422:435	polysaccharide of Hemp seed (HSPc)	422:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	1	26	theme	polysaccharide	179:192	arg1	structure					156:164	structure	156:164	structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	156:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	26	theme	polysaccharide	179:192	arg1	mechanism					301:309	the possible mechanism	288:309	the possible mechanism of this protection	288:328	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	0	27	theme	seed	5:8	arg1	polysaccharides					10:24	Hemp seed polysaccharides	0:24	Hemp seed polysaccharides	0:24	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	6	28	theme	protein	1273:1279	arg1	level					1238:1242	the level	1234:1242	the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis	1234:1316	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	2	29	theme	liquid	491:496	arg1	HPLC					514:517	HPLC	514:517	HPLC	514:517	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	29	theme	liquid	491:496	arg1	chromatography					498:511	high performance liquid chromatography	474:511	high performance liquid chromatography (HPLC)	474:518	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	6	30	theme	factor	1177:1182	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	30	theme	factor	1177:1182	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	0	31	theme	Hemp	0:3	arg1	polysaccharides					10:24	Hemp seed polysaccharides	0:24	Hemp seed polysaccharides	0:24	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	1	32	theme	IPEC-1	271:276	arg1	cells					278:282	IPEC-1 cells	271:282	IPEC-1 cells	271:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	6	33	theme	ECH-associated	1258:1271	arg1	protein					1273:1279	Kelch-like ECH-associated protein 1	1247:1281	Kelch-like ECH-associated protein 1 (Keap1)	1247:1289	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	33	theme	ECH-associated	1258:1271	arg1	Keap1					1284:1288	Keap1	1284:1288	Keap1	1284:1288	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	2	34	from	structure	374:382	arg1	polysaccharide					422:435	polysaccharide	422:435	polysaccharide of Hemp seed (HSPc)	422:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	6	35	theme	Nuclear	1169:1175	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	35	theme	Nuclear	1169:1175	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	2	36	theme	Hemp	440:443	arg1	seed					445:448	Hemp seed	440:448	Hemp seed (HSPc)	440:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	36	theme	Hemp	440:443	arg1	HSPc					451:454	HSPc	451:454	HSPc	451:454	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	1	37	from	damage	261:266	arg1	mechanism					301:309	the possible mechanism	288:309	the possible mechanism of this protection	288:328	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	37	from	damage	261:266	arg1	effect					219:224	the protective effect	204:224	the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	204:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	37	from	damage	261:266	arg1	HSP					195:197	HSP	195:197	HSP	195:197	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	37	from	damage	261:266	arg1	structure					156:164	structure	156:164	structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	156:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	37	from	damage	261:266	arg1	cells					278:282	IPEC-1 cells	271:282	IPEC-1 cells	271:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	37	from	damage	261:266	arg1	polysaccharide					179:192	Hemp seed polysaccharide	169:192	Hemp seed polysaccharide (HSP)	169:198	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	6	38	theme	transcription	1148:1160	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	38	theme	transcription	1148:1160	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	5	39	theme	antioxidant	915:925	arg1	enzymes					927:933	antioxidant enzymes	915:933	antioxidant enzymes	915:933	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	40	theme	enzymes	927:933	arg1	expression					901:910	the gene expression	892:910	the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	892:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	3	41	contain	contain	618:624	arg2	groups					634:639	sulfate groups	626:639	sulfate groups	626:639	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	3	41	contain	contain	618:624	arg1	HSP0.2					611:616	HSP0.2	611:616	HSP0.2	611:616	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	3	41	contain	contain	618:624	arg1	HSP0					602:605	HSP0	602:605	HSP0	602:605	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	3	41	contain	contain	618:624	arg2	polysaccharides					661:675	sulfated polysaccharides	652:675	sulfated polysaccharides	652:675	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	7	42	theme	H2O2-induecd	1423:1434	arg1	stress					1446:1451	H2O2-induecd oxidative stress	1423:1451	H2O2-induecd oxidative stress	1423:1451	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	7	43	theme	oxidative	1436:1444	arg1	stress					1446:1451	H2O2-induecd oxidative stress	1423:1451	H2O2-induecd oxidative stress	1423:1451	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	3	44	theme	sulfate	626:632	arg1	polysaccharides					661:675	sulfated polysaccharides	652:675	sulfated polysaccharides	652:675	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	3	44	theme	sulfate	626:632	arg1	groups					634:639	sulfate groups	626:639	sulfate groups	626:639	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	2	45	theme	monosaccharide	343:356	arg1	composition					358:368	monosaccharide composition	343:368	monosaccharide composition	343:368	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	5	46	theme	phase	939:943	arg1	enzymes					963:969	phase II detoxification enzymes	939:969	phase II detoxification enzymes	939:969	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	0	47	theme	epithelial	45:54	arg1	cells					56:60	intestinal epithelial cells	34:60	intestinal epithelial cells	34:60	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	4	48	theme	cells	688:692	arg1	model					694:698	IPEC-1 cells model	681:698	IPEC-1 cells model	681:698	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	8	49	theme	protection	1459:1468	arg1	mechanism					1470:1478	This protection mechanism	1454:1478	This protection mechanism	1454:1478	This protection mechanism may be related to activation of the Keap1/Nrf2 signaling pathway.
31108145	4	50	theme	SOD	779:781	arg1	activities					765:774	the activities	761:774	the activities of SOD, GSH-Px and CAT	761:797	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	0	51	theme	intestinal	34:43	arg1	cells					56:60	intestinal epithelial cells	34:60	intestinal epithelial cells	34:60	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	1	52	theme	possible	292:299	arg1	mechanism					301:309	the possible mechanism	288:309	the possible mechanism of this protection	288:328	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	7	53	theme	IPEC-1	1402:1407	arg1	cells					1409:1413	IPEC-1 cells	1402:1413	IPEC-1 cells	1402:1413	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	5	54	theme	quantitative	1005:1016	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	54	theme	quantitative	1005:1016	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	7	55	theme	protective	1381:1390	arg1	effect					1392:1397	the protective effect	1377:1397	the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress	1377:1451	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
31108145	1	56	theme	protective	208:217	arg1	effect					219:224	the protective effect	204:224	the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	204:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	4	57	theme	GSH-Px	784:789	arg1	activities					765:774	the activities	761:774	the activities of SOD, GSH-Px and CAT	761:797	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	8	58	theme	Keap1/Nrf2	1516:1525	arg1	pathway					1537:1543	the Keap1/Nrf2 signaling pathway	1512:1543	the Keap1/Nrf2 signaling pathway	1512:1543	This protection mechanism may be related to activation of the Keap1/Nrf2 signaling pathway.
31108145	6	59	theme	intracellular	1134:1146	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	59	theme	intracellular	1134:1146	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	8	60	theme	signaling	1527:1535	arg1	pathway					1537:1543	the Keap1/Nrf2 signaling pathway	1512:1543	the Keap1/Nrf2 signaling pathway	1512:1543	This protection mechanism may be related to activation of the Keap1/Nrf2 signaling pathway.
31108145	4	61	theme	HSP0.2-treated	840:853	arg1	group					855:859	HSP0.2-treated group	840:859	HSP0.2-treated group	840:859	In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP0 and HSP0.2-treated group.
31108145	5	62	theme	chain	1051:1055	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	62	theme	chain	1051:1055	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	63	theme	transcription-polymerase	1026:1049	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	63	theme	transcription-polymerase	1026:1049	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	1	64	theme	study	131:135	arg1	purpose					115:121	The purpose	111:121	The purpose of this study	111:135	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	5	65	theme	real-time	983:991	arg1	qRT-PCR					1067:1073	qRT-PCR	1067:1073	qRT-PCR	1067:1073	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	65	theme	real-time	983:991	arg1	reaction					1057:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction	983:1064	real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	983:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	3	66	theme	sulfated	652:659	arg1	polysaccharides					661:675	sulfated polysaccharides	652:675	sulfated polysaccharides	652:675	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	3	66	theme	sulfated	652:659	arg1	groups					634:639	sulfate groups	626:639	sulfate groups	626:639	The results showed that both HSP0 and HSP0.2 contain sulfate groups, which are sulfated polysaccharides.
31108145	5	67	theme	enzymes	963:969	arg1	expression					901:910	the gene expression	892:910	the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	892:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	6	68	theme	factor	1162:1167	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	68	theme	factor	1162:1167	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	1	69	theme	effect	219:224	arg1	structure					156:164	structure	156:164	structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	156:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	69	theme	effect	219:224	arg1	mechanism					301:309	the possible mechanism	288:309	the possible mechanism of this protection	288:328	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	5	70	theme	detoxification	948:961	arg1	enzymes					963:969	phase II detoxification enzymes	939:969	phase II detoxification enzymes	939:969	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	5	71	theme	gene	896:899	arg1	expression					901:910	the gene expression	892:910	the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR)	892:1074	HSP0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
31108145	0	72	theme	peroxide-induced	76:91	arg1	stress					103:108	hydrogen peroxide-induced oxidative stress	67:108	hydrogen peroxide-induced oxidative stress	67:108	Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress.
31108145	6	73	theme	erythroid-2-related	1184:1202	arg1	factor					1204:1209	intracellular transcription factor Nuclear factor erythroid-2-related factor 2	1134:1211	intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2)	1134:1218	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	6	73	theme	erythroid-2-related	1184:1202	arg1	Nrf2					1214:1217	Nrf2	1214:1217	Nrf2	1214:1217	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	2	74	theme	structure	374:382	arg1	Analysis					331:338	Analysis	331:338	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc)	331:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	75	theme	fractions	391:399	arg1	composition					358:368	monosaccharide composition	343:368	monosaccharide composition	343:368	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	75	theme	fractions	391:399	arg1	structure					374:382	structure	374:382	structure	374:382	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	1	76	theme	HSP	229:231	arg1	effect					219:224	the protective effect	204:224	the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	204:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	76	theme	HSP	229:231	arg1	HSP					195:197	HSP	195:197	HSP	195:197	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	76	theme	HSP	229:231	arg1	polysaccharide					179:192	Hemp seed polysaccharide	169:192	Hemp seed polysaccharide (HSP)	169:198	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	2	77	theme	performance	479:489	arg1	HPLC					514:517	HPLC	514:517	HPLC	514:517	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	77	theme	performance	479:489	arg1	chromatography					498:511	high performance liquid chromatography	474:511	high performance liquid chromatography (HPLC)	474:518	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	1	78	theme	protection	319:328	arg1	structure					156:164	structure	156:164	structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells	156:282	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	1	78	theme	protection	319:328	arg1	mechanism					301:309	the possible mechanism	288:309	the possible mechanism of this protection	288:328	The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H2O2-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection.
31108145	2	79	dep	fractions	391:399	arg1	fractions					391:399	two fractions	387:399	two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc)	387:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	79	dep	fractions	391:399	arg1	HSP0.2					410:415	HSP0.2	410:415	HSP0.2	410:415	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	79	dep	fractions	391:399	arg1	HSP0					401:404	HSP0	401:404	HSP0	401:404	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	80	from	polysaccharide	422:435	arg1	fractions					391:399	two fractions	387:399	two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc)	387:455	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	80	from	polysaccharide	422:435	arg1	composition					358:368	monosaccharide composition	343:368	monosaccharide composition	343:368	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	80	from	polysaccharide	422:435	arg1	HSP0					401:404	HSP0	401:404	HSP0	401:404	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	80	from	polysaccharide	422:435	arg1	HSP0.2					410:415	HSP0.2	410:415	HSP0.2	410:415	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	2	80	from	polysaccharide	422:435	arg1	structure					374:382	structure	374:382	structure	374:382	Analysis of monosaccharide composition and structure of two fractions HSP0 and HSP0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR).
31108145	6	81	with	level	1238:1242	arg1	analysis					1309:1316	Western blot analysis	1296:1316	Western blot analysis	1296:1316	In addition, HSP0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis.
31108145	7	82	theme	cells	1409:1413	arg1	effect					1392:1397	the protective effect	1377:1397	the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress	1377:1451	Collectively, the present study suggested that HSP0.2 has the protective effect of IPEC-1 cells against H2O2-induecd oxidative stress.
29322139	0	0	theme	gut	77:79	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	3	1	theme	control	674:680	arg1	group					682:686	the normal control group	663:686	the normal control group	663:686	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	2	2	theme	treated	573:579	arg1	mice					581:584	CTX treated mice	569:584	CTX treated mice	569:584	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	5	3	theme	short	1363:1367	arg1	acids					1381:1385	these short chain fatty acids	1357:1385	these short chain fatty acids	1357:1385	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	3	4	theme	Lachnospiraceae	764:778	arg1	levels					739:744	increased levels	729:744	increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira	729:795	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	1	5	theme	purple	350:355	arg1	potatoes					363:370	purple sweet potatoes	350:370	purple sweet potatoes	350:370	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	2	6	theme	CTX	569:571	arg1	mice					581:584	CTX treated mice	569:584	CTX treated mice	569:584	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	5	7	theme	acids	1381:1385	arg1	production					1343:1352	the production	1339:1352	the production of these short chain fatty acids in CTX treated mice	1339:1405	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	1	8	theme	sweet	357:361	arg1	potatoes					363:370	purple sweet potatoes	350:370	purple sweet potatoes	350:370	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	3	9	from	levels	812:817	arg1	mice					874:877	normal mice	867:877	normal mice	867:877	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	5	10	theme	normal	1299:1304	arg1	mice					1306:1309	normal mice	1299:1309	normal mice	1299:1309	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	3	11	theme	Bacteroidetes	749:761	arg1	levels					739:744	increased levels	729:744	increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira	729:795	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	0	12	theme	microbiota	81:90	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	5	13	theme	butyric	1283:1289	arg1	acid					1291:1294	butyric acid	1283:1294	butyric acid	1283:1294	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	5	14	theme	CTX	1390:1392	arg1	mice					1402:1405	CTX treated mice	1390:1405	CTX treated mice	1390:1405	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	5	15	from	production	1237:1246	arg1	mice					1306:1309	normal mice	1299:1309	normal mice	1299:1309	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	3	16	theme	Sutterella	853:862	arg1	levels					812:817	levels	812:817	levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice	812:877	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	4	17	theme	polysaccharide	936:949	arg1	groups					961:966	all the three polysaccharide treatment groups	922:966	all the three polysaccharide treatment groups	922:966	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	0	18	theme	normal	107:112	arg1	mice					143:146	normal and cyclophosphamide treated mice	107:146	normal and cyclophosphamide treated mice	107:146	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	0	19	from	potatoes	45:52	arg1	polysaccharides					11:25	polysaccharides	11:25	polysaccharides from purple sweet potatoes	11:52	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	0	19	from	potatoes	45:52	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.	0:147	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	5	20	theme	chain	1369:1373	arg1	acids					1381:1385	these short chain fatty acids	1357:1385	these short chain fatty acids	1357:1385	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	4	21	theme	lower	1090:1094	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	2	22	dep	cytokine	534:541	arg1	IL-6					553:556	IL-6	553:556	IL-6	553:556	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	2	22	dep	cytokine	534:541	arg1	IL-2					544:547	IL-2	544:547	IL-2	544:547	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	3	23	theme	Alcaligenaceae	834:847	arg1	levels					812:817	levels	812:817	levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice	812:877	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	4	24	theme	model	901:905	arg1	group					915:919	a model control group	899:919	a model control group	899:919	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	4	25	theme	treated	1171:1177	arg1	mice					1179:1182	CTX treated mice	1167:1182	CTX treated mice	1167:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	3	26	theme	normal	667:672	arg1	group					682:686	the normal control group	663:686	the normal control group	663:686	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	4	27	from	levels	1096:1101	arg1	mice					1179:1182	CTX treated mice	1167:1182	CTX treated mice	1167:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	2	28	theme	TNF-α	626:630	arg1	levels					616:621	the levels	612:621	the levels of TNF-α	612:630	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	2	28	theme	TNF-α	626:630	arg1	IL-6					642:645	IL-6	642:645	IL-6	642:645	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	2	28	theme	TNF-α	626:630	arg1	IL-2					633:636	IL-2	633:636	IL-2	633:636	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	5	29	theme	propionic	1264:1272	arg1	acid					1274:1277	propionic acid	1264:1277	propionic acid	1264:1277	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	4	30	theme	higher	986:991	arg1	abundances					993:1002	relatively higher abundances	975:1002	relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira	975:1084	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	1	31	theme	concentrated	280:291	arg1	CASP					324:327	CASP	324:327	CASP	324:327	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	1	31	theme	concentrated	280:291	arg1	polysaccharide					308:321	concentrated alkali-soluble polysaccharide	280:321	concentrated alkali-soluble polysaccharide (CASP)	280:328	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	5	32	theme	treated	1394:1400	arg1	mice					1402:1405	CTX treated mice	1390:1405	CTX treated mice	1390:1405	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	4	33	from	mice	1179:1182	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	4	34	theme	Ruminococcus	1056:1067	arg1	abundances					993:1002	relatively higher abundances	975:1002	relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira	975:1084	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	1	35	theme	water-soluble	196:208	arg1	polysaccharide					210:223	water-soluble polysaccharide	196:223	water-soluble polysaccharide (WSP)	196:229	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	1	35	theme	water-soluble	196:208	arg1	WSP					226:228	WSP	226:228	WSP	226:228	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	0	36	theme	polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.	0:147	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	1	37	theme	alkali-soluble	293:306	arg1	CASP					324:327	CASP	324:327	CASP	324:327	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	1	37	theme	alkali-soluble	293:306	arg1	polysaccharide					308:321	concentrated alkali-soluble polysaccharide	280:321	concentrated alkali-soluble polysaccharide (CASP)	280:328	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	2	38	theme	cytokine	534:541	arg1	levels					559:564	immune cytokine (IL-2 and IL-6) levels	527:564	immune cytokine (IL-2 and IL-6) levels	527:564	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	5	39	theme	fatty	1375:1379	arg1	acids					1381:1385	these short chain fatty acids	1357:1385	these short chain fatty acids	1357:1385	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	3	40	theme	normal	867:872	arg1	mice					874:877	normal mice	867:877	normal mice	867:877	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	0	41	theme	sweet	39:43	arg1	potatoes					45:52	purple sweet potatoes	32:52	purple sweet potatoes	32:52	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	2	42	theme	immune	527:532	arg1	cytokine					534:541	immune cytokine	527:541	immune cytokine (IL-2 and IL-6) levels	527:564	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	4	43	theme	Oscillospira	1073:1084	arg1	abundances					993:1002	relatively higher abundances	975:1002	relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira	975:1084	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	0	44	theme	purple	32:37	arg1	potatoes					45:52	purple sweet potatoes	32:52	purple sweet potatoes	32:52	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	3	45	theme	treatment	702:710	arg1	groups					712:717	CASP treatment groups	697:717	CASP treatment groups	697:717	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	0	46	theme	treated	135:141	arg1	mice					143:146	normal and cyclophosphamide treated mice	107:146	normal and cyclophosphamide treated mice	107:146	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	0	47	from	response	64:71	arg1	mice					143:146	normal and cyclophosphamide treated mice	107:146	normal and cyclophosphamide treated mice	107:146	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	4	48	theme	Sutterella	1153:1162	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	3	49	theme	increased	729:737	arg1	levels					739:744	increased levels	729:744	increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira	729:795	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	4	50	theme	treatment	951:959	arg1	groups					961:966	all the three polysaccharide treatment groups	922:966	all the three polysaccharide treatment groups	922:966	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	4	51	theme	control	907:913	arg1	group					915:919	a model control group	899:919	a model control group	899:919	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	2	52	theme	spleen	510:515	arg1	index					517:521	the spleen index	506:521	the spleen index	506:521	The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6.
29322139	0	53	from	Effects	0:6	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	0	53	from	Effects	0:6	arg1	potatoes					45:52	purple sweet potatoes	32:52	purple sweet potatoes	32:52	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	0	53	from	Effects	0:6	arg1	response					64:71	immune response	57:71	immune response	57:71	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	5	54	theme	acid	1291:1294	arg1	production					1237:1246	the production	1233:1246	the production of acetic acid, propionic acid and butyric acid in normal mice	1233:1309	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	4	55	theme	CTX	1167:1169	arg1	mice					1179:1182	CTX treated mice	1167:1182	CTX treated mice	1167:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	3	56	theme	CASP	697:700	arg1	groups					712:717	CASP treatment groups	697:717	CASP treatment groups	697:717	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	5	57	theme	acetic	1251:1256	arg1	acid					1258:1261	acetic acid	1251:1261	acetic acid	1251:1261	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	0	58	from	composition	92:102	arg1	mice					143:146	normal and cyclophosphamide treated mice	107:146	normal and cyclophosphamide treated mice	107:146	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	4	59	theme	Proteobacteria	1118:1131	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	5	60	theme	acid	1274:1277	arg1	production					1237:1246	the production	1233:1246	the production of acetic acid, propionic acid and butyric acid in normal mice	1233:1309	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	3	61	theme	Oscillospira	784:795	arg1	levels					739:744	increased levels	729:744	increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira	729:795	As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice.
29322139	4	62	theme	Firmicutes	1106:1115	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	4	63	dep	Lachnospiraceae	1039:1053	arg1	Ruminococcaceae					1022:1036	Ruminococcaceae	1022:1036	Ruminococcaceae	1022:1036	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	1	64	theme	treated	431:437	arg1	mice					439:442	treated mice	431:442	treated mice	431:442	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	4	65	from	Proteobacteria	1118:1131	arg1	mice					1179:1182	CTX treated mice	1167:1182	CTX treated mice	1167:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	5	66	from	production	1343:1352	arg1	mice					1402:1405	CTX treated mice	1390:1405	CTX treated mice	1390:1405	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	4	67	theme	Lachnospiraceae	1039:1053	arg1	abundances					993:1002	relatively higher abundances	975:1002	relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira	975:1084	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	1	68	theme	dilute	232:237	arg1	DASP					270:273	DASP	270:273	DASP	270:273	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	1	68	theme	dilute	232:237	arg1	polysaccharide					254:267	dilute alkali-soluble polysaccharide	232:267	dilute alkali-soluble polysaccharide (DASP)	232:274	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	0	69	theme	immune	57:62	arg1	response					64:71	immune response	57:71	immune response	57:71	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	1	70	dep	normal	397:402	arg1	mice					439:442	treated mice	431:442	treated mice	431:442	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	4	71	theme	Alcaligenaceae	1134:1147	arg1	levels					1096:1101	lower levels	1090:1101	lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice	1090:1182	When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice.
29322139	0	72	dep	treated	135:141	arg1	cyclophosphamide					118:133	cyclophosphamide	118:133	cyclophosphamide	118:133	Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice.
29322139	5	73	theme	acid	1258:1261	arg1	production					1237:1246	the production	1233:1246	the production of acetic acid, propionic acid and butyric acid in normal mice	1233:1309	Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
29322139	1	74	theme	alkali-soluble	239:252	arg1	DASP					270:273	DASP	270:273	DASP	270:273	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
29322139	1	74	theme	alkali-soluble	239:252	arg1	polysaccharide					254:267	dilute alkali-soluble polysaccharide	232:267	dilute alkali-soluble polysaccharide (DASP)	232:274	In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage.
30658444	0	0	theme	Proposed	74:81	arg1	Biosimilar					94:103	a Proposed Etanercept Biosimilar	72:103	a Proposed Etanercept Biosimilar	72:103	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	0	0	theme	Proposed	74:81	arg1	Altebrel™					61:69	Altebrel™	61:69	Altebrel™	61:69	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	9	1	theme	significant	1430:1440	arg1	effect					1442:1447	no significant effect	1427:1447	no significant effect	1427:1447	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	10	2	theme	in-vitro	1599:1606	arg1	functionality					1608:1620	in-vitro functionality	1599:1620	in-vitro functionality	1599:1620	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	10	2	theme	in-vitro	1599:1606	arg1	glycosylation					1562:1574	glycosylation	1562:1574	glycosylation	1562:1574	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	3	3	theme	current	503:509	arg1	study					511:515	the current study	499:515	the current study	499:515	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	6	4	theme	peptide	942:948	arg1	mapping					950:956	Three enzyme peptide mapping	929:956	Three enzyme peptide mapping	929:956	Three enzyme peptide mapping resulted in complete identification of the primary structure.
30658444	10	5	theme	high	1534:1537	arg1	similarity					1539:1548	a high similarity	1532:1548	a high similarity in terms of glycosylation, primary structure and in-vitro functionality	1532:1620	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	10	6	theme	etanercept	1492:1501	arg1	Analysis					1480:1487	Analysis	1480:1487	Analysis	1480:1487	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	4	7	theme	primary	677:683	arg1	analysis					694:701	primary sequence analysis	677:701	primary sequence analysis	677:701	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	8	gly	N/O-glycosylation	718:734	arg2	sites					736:740	N/O-glycosylation sites	718:740	N/O-glycosylation sites	718:740	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	6	9	theme	enzyme	935:940	arg1	mapping					950:956	Three enzyme peptide mapping	929:956	Three enzyme peptide mapping	929:956	Three enzyme peptide mapping resulted in complete identification of the primary structure.
30658444	2	10	theme	structural	293:302	arg1	properties					329:338	physicochemical, structural, functional and clinical properties	276:338	physicochemical, structural, functional and clinical properties	276:338	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	0	11	theme	Etanercept	83:92	arg1	Biosimilar					94:103	a Proposed Etanercept Biosimilar	72:103	a Proposed Etanercept Biosimilar	72:103	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	0	11	theme	Etanercept	83:92	arg1	Altebrel™					61:69	Altebrel™	61:69	Altebrel™	61:69	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	4	12	theme	sequence	685:692	arg1	analysis					694:701	primary sequence analysis	677:701	primary sequence analysis	677:701	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	5	13	theme	in-vitro	879:886	arg1	functionality					888:900	in-vitro functionality	879:900	in-vitro functionality of etanercept	879:914	N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed.
30658444	9	14	gly	N-deglycosylated	1279:1294	arg1	samples					1324:1330	N-deglycosylated and non-modified etanercept samples	1279:1330	N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1279:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	7	15	theme	biodrugs	1133:1140	arg1	structure					1120:1128	the primary structure	1108:1128	the primary structure of biodrugs	1108:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	4	16	theme	sites	736:740	arg1	quantification					746:759	quantification	746:759	quantification of methionine oxidation	746:783	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	16	theme	sites	736:740	arg1	evaluation					704:713	evaluation	704:713	evaluation of N/O-glycosylation sites	704:740	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	16	theme	sites	736:740	arg1	analysis					694:701	primary sequence analysis	677:701	primary sequence analysis	677:701	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	2	17	theme	physicochemical	276:290	arg1	properties					329:338	physicochemical, structural, functional and clinical properties	276:338	physicochemical, structural, functional and clinical properties	276:338	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	4	18	theme	spectrometric	641:653	arg1	analysis					655:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	2	19	contain	has	368:370	arg1	biosimilar					357:366	a biosimilar	355:366	a biosimilar	355:366	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	2	19	contain	has	368:370	arg2	differences					387:397	no significant differences	372:397	no significant differences	372:397	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	2	20	theme	clinical	479:486	arg1	trials					488:493	extensive clinical trials	469:493	extensive clinical trials	469:493	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	4	21	theme	oxidation	775:783	arg1	quantification					746:759	quantification	746:759	quantification of methionine oxidation	746:783	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	21	theme	oxidation	775:783	arg1	evaluation					704:713	evaluation	704:713	evaluation of N/O-glycosylation sites	704:740	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	21	theme	oxidation	775:783	arg1	analysis					694:701	primary sequence analysis	677:701	primary sequence analysis	677:701	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	9	22	theme	in-vitro	1456:1463	arg1	functionality					1465:1477	its in-vitro functionality	1452:1477	its in-vitro functionality	1452:1477	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	1	23	theme	reliable	166:173	arg1	studies					189:195	reliable comparability studies	166:195	reliable comparability studies of biosimilars	166:210	The demand for reliable comparability studies of biosimilars grows with their increased market share.
30658444	5	24	theme	permethylation	818:831	arg1	derivatization					833:846	permethylation derivatization	818:846	permethylation derivatization	818:846	N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed.
30658444	7	25	theme	structure	1120:1128	arg1	analysis					1096:1103	the analysis	1092:1103	the analysis of the primary structure of biodrugs	1092:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	9	26	theme	functional	1254:1263	arg1	properties					1265:1274	the functional properties	1250:1274	the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1250:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	2	27	theme	clinical	320:327	arg1	properties					329:338	physicochemical, structural, functional and clinical properties	276:338	physicochemical, structural, functional and clinical properties	276:338	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	1	28	theme	comparability	175:187	arg1	studies					189:195	reliable comparability studies	166:195	reliable comparability studies of biosimilars	166:210	The demand for reliable comparability studies of biosimilars grows with their increased market share.
30658444	0	29	theme	Structural	0:9	arg1	Analysis					49:56	Structural and In Vitro Functional Comparability Analysis	0:56	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.	0:149	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	4	30	used	used	668:671	arg2	analysis					655:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	4	31	theme	N/O-glycosylation	718:734	arg1	sites					736:740	N/O-glycosylation sites	718:740	N/O-glycosylation sites	718:740	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	2	32	theme	significant	375:385	arg1	differences					387:397	no significant differences	372:397	no significant differences	372:397	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	6	33	theme	structure	1009:1017	arg1	identification					979:992	complete identification	970:992	complete identification of the primary structure	970:1017	Three enzyme peptide mapping resulted in complete identification of the primary structure.
30658444	0	34	theme	Primary	115:121	arg1	Sequence					123:130	Primary Sequence	115:130	Primary Sequence	115:130	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	2	35	theme	functional	305:314	arg1	properties					329:338	physicochemical, structural, functional and clinical properties	276:338	physicochemical, structural, functional and clinical properties	276:338	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	9	36	theme	properties	1265:1274	arg1	investigation					1233:1245	investigation	1233:1245	investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1233:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	6	37	theme	primary	1001:1007	arg1	structure					1009:1017	the primary structure	997:1017	the primary structure	997:1017	Three enzyme peptide mapping resulted in complete identification of the primary structure.
30658444	7	38	theme	primary	1112:1118	arg1	structure					1120:1128	the primary structure	1108:1128	the primary structure of biodrugs	1108:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	9	39	theme	N-deglycosylated	1279:1294	arg1	samples					1324:1330	N-deglycosylated and non-modified etanercept samples	1279:1330	N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1279:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	3	40	dep	Enbrel®	518:524	arg1	etanercept					527:536	etanercept	527:536	etanercept	527:536	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	3	40	dep	Enbrel®	518:524	arg1	originator					543:552	the originator	539:552	the originator	539:552	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	1	41	theme	biosimilars	200:210	arg1	studies					189:195	reliable comparability studies	166:195	reliable comparability studies of biosimilars	166:210	The demand for reliable comparability studies of biosimilars grows with their increased market share.
30658444	0	42	theme	Comparability	35:47	arg1	Analysis					49:56	Structural and In Vitro Functional Comparability Analysis	0:56	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.	0:149	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	10	43	theme	primary	1577:1583	arg1	structure					1585:1593	primary structure	1577:1593	primary structure	1577:1593	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	10	43	theme	primary	1577:1583	arg1	glycosylation					1562:1574	glycosylation	1562:1574	glycosylation	1562:1574	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	5	44	theme	N-glycans	866:874	arg1	effect					856:861	the effect	852:861	the effect of N-glycans on in-vitro functionality of etanercept	852:914	N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed.
30658444	3	45	theme	proposed	574:581	arg1	biosimilar					583:592	the proposed biosimilar	570:592	the proposed biosimilar	570:592	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	3	45	theme	proposed	574:581	arg1	Altebrel™					559:567	Altebrel™	559:567	Altebrel™	559:567	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	5	46	theme	etanercept	905:914	arg1	functionality					888:900	in-vitro functionality	879:900	in-vitro functionality of etanercept	879:914	N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed.
30658444	4	47	theme	"	634:634	arg1	analysis					655:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	0	48	theme	Functional	24:33	arg1	Analysis					49:56	Structural and In Vitro Functional Comparability Analysis	0:56	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.	0:149	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	2	49	theme	extensive	469:477	arg1	trials					488:493	extensive clinical trials	469:493	extensive clinical trials	469:493	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	9	50	theme	non-modified	1300:1311	arg1	samples					1324:1330	N-deglycosylated and non-modified etanercept samples	1279:1330	N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1279:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	0	51	dep	Analysis	49:56	arg1	Focus					106:110	Focus	106:110	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.	0:149	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	2	52	theme	originator	406:415	arg1	product					417:423	the originator product	402:423	the originator product	402:423	These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials.
30658444	10	53	theme	biosimilar	1511:1520	arg1	Analysis					1480:1487	Analysis	1480:1487	Analysis	1480:1487	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	8	54	theme	N/O-glycan	1147:1156	arg1	structures					1158:1167	New N/O-glycan structures	1143:1167	New N/O-glycan structures	1143:1167	New N/O-glycan structures were identified and all the N-glycans were quantified.
30658444	9	55	theme	etanercept	1313:1322	arg1	samples					1324:1330	N-deglycosylated and non-modified etanercept samples	1279:1330	N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1279:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	7	56	theme	total	1042:1046	arg1	chromatograms					1052:1064	total ion chromatograms	1042:1064	total ion chromatograms	1042:1064	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	7	56	theme	total	1042:1046	arg1	datasets					1079:1086	valuable datasets	1070:1086	valuable datasets for the analysis of the primary structure of biodrugs	1070:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	9	57	theme	in-vitro	1377:1384	arg1	bioassay					1386:1393	in-vitro bioassay	1377:1393	in-vitro bioassay	1377:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	6	58	theme	complete	970:977	arg1	identification					979:992	complete identification	970:992	complete identification of the primary structure	970:1017	Three enzyme peptide mapping resulted in complete identification of the primary structure.
30658444	8	59	theme	New	1143:1145	arg1	structures					1158:1167	New N/O-glycan structures	1143:1167	New N/O-glycan structures	1143:1167	New N/O-glycan structures were identified and all the N-glycans were quantified.
30658444	0	60	from	Focus	106:110	arg1	Sequence					123:130	Primary Sequence	115:130	Primary Sequence	115:130	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	0	60	from	Focus	106:110	arg1	Glycosylation					136:148	Glycosylation	136:148	Glycosylation	136:148	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	5	61	from	effect	856:861	arg1	functionality					888:900	in-vitro functionality	879:900	in-vitro functionality of etanercept	879:914	N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed.
30658444	7	62	theme	valuable	1070:1077	arg1	chromatograms					1052:1064	total ion chromatograms	1042:1064	total ion chromatograms	1042:1064	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	7	62	theme	valuable	1070:1077	arg1	datasets					1079:1086	valuable datasets	1070:1086	valuable datasets for the analysis of the primary structure of biodrugs	1070:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	3	63	theme	direct	605:610	arg1	comparison					612:621	direct comparison	605:621	direct comparison	605:621	In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison.
30658444	7	64	theme	ion	1048:1050	arg1	chromatograms					1052:1064	total ion chromatograms	1042:1064	total ion chromatograms	1042:1064	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	7	64	theme	ion	1048:1050	arg1	datasets					1079:1086	valuable datasets	1070:1086	valuable datasets for the analysis of the primary structure of biodrugs	1070:1140	It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs.
30658444	9	65	contain	has	1423:1425	arg2	effect					1442:1447	no significant effect	1427:1447	no significant effect	1427:1447	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	9	65	contain	has	1423:1425	arg1	N-glycosylation					1407:1421	N-glycosylation	1407:1421	N-glycosylation	1407:1421	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	9	66	theme	surface	1338:1344	arg1	resonance					1354:1362	surface plasmon resonance	1338:1362	surface plasmon resonance analysis	1338:1371	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	4	67	theme	methionine	764:773	arg1	oxidation					775:783	methionine oxidation	764:783	methionine oxidation	764:783	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	10	68	theme	glycosylation	1562:1574	arg1	terms					1553:1557	terms	1553:1557	terms of glycosylation, primary structure and in-vitro functionality	1553:1620	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30658444	9	69	theme	samples	1324:1330	arg1	properties					1265:1274	the functional properties	1250:1274	the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay	1250:1393	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	1	70	theme	increased	229:237	arg1	share					246:250	their increased market share	223:250	their increased market share	223:250	The demand for reliable comparability studies of biosimilars grows with their increased market share.
30658444	9	71	theme	plasmon	1346:1352	arg1	resonance					1354:1362	surface plasmon resonance	1338:1362	surface plasmon resonance analysis	1338:1371	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	4	72	theme	mass	636:639	arg1	analysis					655:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis	624:662	"Bottom-up" mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation.
30658444	1	73	theme	market	239:244	arg1	share					246:250	their increased market share	223:250	their increased market share	223:250	The demand for reliable comparability studies of biosimilars grows with their increased market share.
30658444	0	74	theme	Altebrel™	61:69	arg1	Analysis					49:56	Structural and In Vitro Functional Comparability Analysis	0:56	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.	0:149	Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.
30658444	9	75	theme	resonance	1354:1362	arg1	analysis					1364:1371	surface plasmon resonance analysis	1338:1371	surface plasmon resonance analysis	1338:1371	Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality.
30658444	10	76	from	similarity	1539:1548	arg1	terms					1553:1557	terms	1553:1557	terms of glycosylation, primary structure and in-vitro functionality	1553:1620	Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.
30156756	0	0	theme	Muscle	86:91	arg1	Tissue					93:98	Highly Aligned Muscle Tissue	71:98	Highly Aligned Muscle Tissue	71:98	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	4	1	theme	GelMA	504:508	arg1	hydrogels					510:518	GelMA hydrogels	504:518	GelMA hydrogels	504:518	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	4	2	from	versatility	525:535	arg1	properties					557:566	their mechanical properties	540:566	their mechanical properties	540:566	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	4	2	from	versatility	525:535	arg1	structures					583:592	lasting 3D structures	572:592	lasting 3D structures	572:592	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	7	3	theme	hydrogels	1020:1028	arg1	properties					983:992	Physical properties	974:992	Physical properties of the obtained composite hydrogels	974:1028	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	0	4	theme	Aligned	78:84	arg1	Tissue					93:98	Highly Aligned Muscle Tissue	71:98	Highly Aligned Muscle Tissue	71:98	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	7	5	theme	pristine	1157:1164	arg1	GelMA					1166:1170	pristine GelMA	1157:1170	pristine GelMA	1157:1170	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	7	6	theme	obtained	1001:1008	arg1	hydrogels					1020:1028	the obtained composite hydrogels	997:1028	the obtained composite hydrogels	997:1028	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	5	7	theme	versatile	655:663	arg1	scaffolds					700:708	versatile, lasting, and mechanically tunable scaffolds	655:708	versatile, lasting, and mechanically tunable scaffolds	655:708	In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented.
30156756	5	8	theme	lasting	666:672	arg1	scaffolds					700:708	versatile, lasting, and mechanically tunable scaffolds	655:708	versatile, lasting, and mechanically tunable scaffolds	655:708	In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented.
30156756	6	9	theme	synthetic	843:851	arg1	material					853:860	a synthetic material	841:860	a synthetic material	841:860	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	0	10	theme	Tissue	93:98	arg1	Bioprinting					56:66	3D Bioprinting	53:66	3D Bioprinting of Highly Aligned Muscle Tissue	53:98	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	2	11	theme	photopolymerizable	273:290	arg1	hydrogel					292:299	a photopolymerizable hydrogel	271:299	a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride	271:369	Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride.
30156756	2	11	theme	photopolymerizable	273:290	arg1	Gelatinmethacryloyl					240:258	Gelatinmethacryloyl	240:258	Gelatinmethacryloyl (GelMA)	240:266	Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride.
30156756	9	12	theme	biocompatible	1337:1349	arg1	biomaterials					1389:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	5	13	theme	composite	622:630	arg1	biomaterials					632:643	composite biomaterials	622:643	composite biomaterials	622:643	In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented.
30156756	9	14	theme	myotube	1502:1508	arg1	formation					1510:1518	myotube formation	1502:1518	myotube formation	1502:1518	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	1	15	theme	complex	168:174	arg1	muscle					232:237	skeletal and cardiac muscle	211:237	muscle	232:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	1	15	theme	complex	168:174	arg1	tissues					194:200	complex functional living tissues	168:200	complex functional living tissues	168:200	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	0	16	theme	3D	53:54	arg1	Bioprinting					56:66	3D Bioprinting	53:66	3D Bioprinting of Highly Aligned Muscle Tissue	53:98	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	9	17	theme	novel	1351:1355	arg1	biomaterials					1389:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	5	18	theme	biomaterials	632:643	arg1	library					611:617	a library	609:617	a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds	609:708	In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented.
30156756	1	19	theme	functional	176:185	arg1	muscle					232:237	skeletal and cardiac muscle	211:237	muscle	232:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	1	19	theme	functional	176:185	arg1	tissues					194:200	complex functional living tissues	168:200	complex functional living tissues	168:200	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	9	20	theme	biomaterials	1389:1400	arg1	library					1326:1332	a library	1324:1332	a library of biocompatible novel and totally versatile composite biomaterials	1324:1400	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	7	21	theme	C2C12	1119:1123	arg1	cells					1132:1136	C2C12 murine cells	1119:1136	C2C12 murine cells	1119:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	7	22	theme	Physical	974:981	arg1	properties					983:992	Physical properties	974:992	Physical properties of the obtained composite hydrogels	974:1028	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	1	23	theme	living	187:192	arg1	muscle					232:237	skeletal and cardiac muscle	211:237	muscle	232:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	1	23	theme	living	187:192	arg1	tissues					194:200	complex functional living tissues	168:200	complex functional living tissues	168:200	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	0	24	theme	Composite	0:8	arg1	Biomaterials					10:21	Composite Biomaterials	0:21	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.	0:99	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	4	25	theme	lasting	572:578	arg1	structures					583:592	lasting 3D structures	572:592	lasting 3D structures	572:592	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	6	26	theme	hydrogel	957:964	arg1	blends					966:971	photopolymerizable hydrogel blends	938:971	photopolymerizable hydrogel blends	938:971	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	6	27	theme	methacrylic	814:824	arg1	anhydride					826:834	methacrylic anhydride	814:834	methacrylic anhydride	814:834	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	2	28	theme	natural	313:319	arg1	gelatin					321:327	natural gelatin	313:327	natural gelatin functionalized with methacrylic anhydride	313:369	Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride.
30156756	1	29	theme	tissues	194:200	arg1	printing					156:163	the direct 3D printing	142:163	the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle	142:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	0	30	theme	Long-Lasting	26:37	arg1	Scaffolds					39:47	Long-Lasting Scaffolds	26:47	Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue	26:98	Composite Biomaterials as Long-Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue.
30156756	8	31	theme	high	1199:1202	arg1	resistance					1204:1213	high resistance	1199:1213	high resistance to degradation maintaining the 3D structure with high fidelity over several weeks	1199:1295	All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks.
30156756	7	32	theme	fibers	1107:1112	arg1	development					1076:1086	development	1076:1086	development	1076:1086	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	7	32	theme	fibers	1107:1112	arg1	growth					1065:1070	growth	1065:1070	growth	1065:1070	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	1	33	theme	New	101:103	arg1	materials					119:127	New biocompatible materials	101:127	New biocompatible materials	101:127	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	3	34	theme	tissue	469:474	arg1	engineering					476:486	tissue engineering	469:486	tissue engineering	469:486	However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering.
30156756	7	35	from	cells	1132:1136	arg1	development					1076:1086	development	1076:1086	development	1076:1086	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	7	35	from	cells	1132:1136	arg1	growth					1065:1070	growth	1065:1070	growth	1065:1070	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	7	35	from	cells	1132:1136	arg1	fibers					1107:1112	skeletal muscle fibers	1091:1112	skeletal muscle fibers from C2C12 murine cells	1091:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	1	36	theme	biocompatible	105:117	arg1	materials					119:127	New biocompatible materials	101:127	New biocompatible materials	101:127	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	7	37	theme	murine	1125:1130	arg1	cells					1132:1136	C2C12 murine cells	1119:1136	C2C12 murine cells	1119:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	4	38	theme	3D	580:581	arg1	structures					583:592	lasting 3D structures	572:592	lasting 3D structures	572:592	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	7	39	theme	skeletal	1091:1098	arg1	fibers					1107:1112	skeletal muscle fibers	1091:1112	skeletal muscle fibers from C2C12 murine cells	1091:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	8	40	theme	several	1283:1289	arg1	weeks					1291:1295	several weeks	1283:1295	several weeks	1283:1295	All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks.
30156756	9	41	theme	composite	1379:1387	arg1	biomaterials					1389:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	8	42	theme	3D	1246:1247	arg1	structure					1249:1257	the 3D structure	1242:1257	the 3D structure with high fidelity	1242:1276	All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks.
30156756	7	43	theme	muscle	1100:1105	arg1	fibers					1107:1112	skeletal muscle fibers	1091:1112	skeletal muscle fibers from C2C12 murine cells	1091:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	6	44	theme	ethylene	876:883	arg1	poly					871:874	poly	871:874	poly(ethylene glycol) diacrylate	871:902	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	6	44	theme	ethylene	876:883	arg1	glycol					885:890	ethylene glycol	876:890	ethylene glycol	876:890	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	9	45	theme	tunable	1440:1446	arg1	properties					1459:1468	tunable mechanical properties	1440:1468	tunable mechanical properties that give structure and support myotube formation and alignment	1440:1532	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	1	46	theme	skeletal	211:218	arg1	muscle					232:237	skeletal and cardiac muscle	211:237	muscle	232:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	8	47	theme	high	1264:1267	arg1	fidelity					1269:1276	high fidelity	1264:1276	high fidelity	1264:1276	All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks.
30156756	9	48	theme	versatile	1369:1377	arg1	biomaterials					1389:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	biocompatible novel and totally versatile composite biomaterials	1337:1400	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
30156756	3	49	theme	single	409:414	arg1	hydrogel					416:423	a single hydrogel	407:423	a single hydrogel that meets all the desirable properties for tissue engineering	407:486	However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering.
30156756	6	50	theme	poly	871:874	arg1	diacrylate					893:902	poly(ethylene glycol) diacrylate	871:902	poly(ethylene glycol) diacrylate	871:902	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	2	51	theme	methacrylic	349:359	arg1	anhydride					361:369	methacrylic anhydride	349:369	methacrylic anhydride	349:369	Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride.
30156756	4	52	theme	mechanical	546:555	arg1	properties					557:566	their mechanical properties	540:566	their mechanical properties	540:566	In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures.
30156756	5	53	theme	tunable	692:698	arg1	scaffolds					700:708	versatile, lasting, and mechanically tunable scaffolds	655:708	versatile, lasting, and mechanically tunable scaffolds	655:708	In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented.
30156756	6	54	theme	carboxymethyl	759:771	arg1	cellulose					773:781	carboxymethyl cellulose	759:781	carboxymethyl cellulose	759:781	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	8	55	with	structure	1249:1257	arg1	fidelity					1269:1276	high fidelity	1264:1276	high fidelity	1264:1276	All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks.
30156756	7	56	from	growth	1065:1070	arg1	cells					1132:1136	C2C12 murine cells	1119:1136	C2C12 murine cells	1119:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	1	57	theme	cardiac	224:230	arg1	muscle					232:237	skeletal and cardiac muscle	211:237	muscle	232:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	1	58	theme	direct	146:151	arg1	printing					156:163	the direct 3D printing	142:163	the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle	142:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	3	59	theme	desirable	444:452	arg1	properties					454:463	all the desirable properties	436:463	all the desirable properties for tissue engineering	436:486	However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering.
30156756	7	60	from	development	1076:1086	arg1	cells					1132:1136	C2C12 murine cells	1119:1136	C2C12 murine cells	1119:1136	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	6	61	theme	photopolymerizable	938:955	arg1	blends					966:971	photopolymerizable hydrogel blends	938:971	photopolymerizable hydrogel blends	938:971	Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends.
30156756	7	62	theme	composite	1010:1018	arg1	hydrogels					1020:1028	the obtained composite hydrogels	997:1028	the obtained composite hydrogels	997:1028	Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA.
30156756	1	63	theme	3D	153:154	arg1	printing					156:163	the direct 3D printing	142:163	the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle	142:237	New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle.
30156756	9	64	theme	mechanical	1448:1457	arg1	properties					1459:1468	tunable mechanical properties	1440:1468	tunable mechanical properties that give structure and support myotube formation and alignment	1440:1532	Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
31712159	7	0	from	muricatum	1129:1137	arg1	polysaccharide					1101:1114	the neutral polysaccharide	1089:1114	the neutral polysaccharide from Solanum muricatum	1089:1137	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	1	1	theme	neutral	150:156	arg1	SMP-0b					173:178	a novel neutral polysaccharide SMP-0b	142:178	a novel neutral polysaccharide SMP-0b	142:178	In the present study, a novel neutral polysaccharide SMP-0b was extracted and purified from the pulp of Solanum muricatum.
31712159	5	2	theme	backbone	709:716	arg1	chain					718:722	the backbone chain	705:722	the backbone chain of SMP-0b	705:732	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	1	3	theme	polysaccharide	158:171	arg1	SMP-0b					173:178	a novel neutral polysaccharide SMP-0b	142:178	a novel neutral polysaccharide SMP-0b	142:178	In the present study, a novel neutral polysaccharide SMP-0b was extracted and purified from the pulp of Solanum muricatum.
31712159	4	4	theme	SMP-0b	632:637	arg1	structure					619:627	The structure	615:627	The structure of SMP-0b	615:637	The structure of SMP-0b was characterized by methylation and NMR analysis.
31712159	5	5	theme	1→	765:766	arg1	-α-d-Glcp-					795:804	→4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→	751:807	-α-d-Glcp-	795:804	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	6	6	theme	immunomodulation	883:898	arg1	assays					900:905	immunomodulation assays	883:905	immunomodulation assays in vitro	883:914	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	3	7	theme	permeation	588:597	arg1	chromatography					599:612	high performance gel permeation chromatography	567:612	high performance gel permeation chromatography	567:612	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	7	8	theme	functional	1199:1208	arg1	food					1210:1213	functional food	1199:1213	functional food to enhance immunity	1199:1233	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	7	9	theme	neutral	1093:1099	arg1	polysaccharide					1101:1114	the neutral polysaccharide	1089:1114	the neutral polysaccharide from Solanum muricatum	1089:1137	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	7	10	contain	have	1145:1148	arg1	polysaccharide					1101:1114	the neutral polysaccharide	1089:1114	the neutral polysaccharide from Solanum muricatum	1089:1137	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	7	10	contain	have	1145:1148	arg2	potential					1150:1158	potential	1150:1158	potential	1150:1158	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	6	11	theme	immunomodulatory	939:954	arg1	activity					956:963	good immunomodulatory activity	934:963	good immunomodulatory activity	934:963	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	5	12	dep	-α-d-Glcp-	795:804	arg1	→3,6					769:772	→4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→	751:807	→3,6	769:772	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	5	12	dep	-α-d-Glcp-	795:804	arg1	→6					792:793	→6	792:793	→6	792:793	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	5	12	dep	-α-d-Glcp-	795:804	arg1	1→					785:786	1→	785:786	1→	785:786	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	3	13	theme	performance	572:582	arg1	chromatography					599:612	high performance gel permeation chromatography	567:612	high performance gel permeation chromatography	567:612	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	3	14	theme	molecular	475:483	arg1	weight					485:490	number-average molecular weight	460:490	number-average molecular weight	460:490	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	3	15	theme	gel	584:586	arg1	chromatography					599:612	high performance gel permeation chromatography	567:612	high performance gel permeation chromatography	567:612	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	2	16	theme	5.31:2.92:42.23:25.38	397:417	arg1	ratio					388:392	the molar ratio	378:392	the molar ratio of 5.31:2.92:42.23:25.38	378:417	Monosaccharide composition analysis revealed that SMP-0b was mainly composed of l-arabinose, d-mannose, d-glucose and d-galactose with the molar ratio of 5.31:2.92:42.23:25.38.
31712159	7	17	from	immunomodulator	1166:1180	arg1	food					1210:1213	functional food	1199:1213	functional food to enhance immunity	1199:1233	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	5	18	theme	-β-d-Manp-	774:783	arg1	-α-d-Glcp-					795:804	→4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→	751:807	-α-d-Glcp-	795:804	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	6	19	theme	macrophage	1044:1053	arg1	cells					1055:1059	RAW 264.7 macrophage cells	1034:1059	RAW 264.7 macrophage cells	1034:1059	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	6	20	theme	good	934:937	arg1	activity					956:963	good immunomodulatory activity	934:963	good immunomodulatory activity	934:963	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	3	21	dep	weight	449:454	arg1	The					420:422	The	420:422	The	420:422	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	5	22	dep	-α-d-Glcp-	865:874	arg1	→4					862:863	→4	862:863	→4	862:863	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	5	22	dep	-α-d-Glcp-	865:874	arg1	1→					855:856	1→	855:856	1→	855:856	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	3	23	theme	number-average	460:473	arg1	weight					485:490	number-average molecular weight	460:490	number-average molecular weight	460:490	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	0	24	theme	immunomodulatory	31:46	arg1	activity					48:55	immunomodulatory activity	31:55	immunomodulatory activity	31:55	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	6	25	theme	cells	1055:1059	arg1	proliferation					999:1011	proliferation	999:1011	proliferation	999:1011	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	6	25	theme	cells	1055:1059	arg1	production					1020:1029	NO production	1017:1029	NO production	1017:1029	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	5	26	theme	side	818:821	arg1	chain					823:827	the side chain	814:827	the side chain	814:827	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	3	27	theme	molecular	439:447	arg1	weight					449:454	weight-average molecular weight	424:454	weight-average molecular weight	424:454	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	5	28	theme	-β-d-Galp-	754:763	arg1	-α-d-Glcp-					795:804	→4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→	751:807	-α-d-Glcp-	795:804	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	7	29	from	supplement	1185:1194	arg1	food					1210:1213	functional food	1199:1213	functional food to enhance immunity	1199:1233	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	3	30	theme	SMP-0b	495:500	arg1	weight					485:490	number-average molecular weight	460:490	number-average molecular weight	460:490	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	3	30	theme	SMP-0b	495:500	arg1	weight					449:454	weight-average molecular weight	424:454	weight-average molecular weight	424:454	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	0	31	theme	new	62:64	arg1	polysaccharide					74:87	a new neutral polysaccharide	60:87	a new neutral polysaccharide	60:87	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	6	32	theme	in	907:908	arg1	assays					900:905	immunomodulation assays	883:905	immunomodulation assays in vitro	883:914	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	5	33	theme	SMP-0b	727:732	arg1	chain					718:722	the backbone chain	705:722	the backbone chain of SMP-0b	705:732	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	6	34	dep	in	907:908	arg1	vitro					910:914	vitro	910:914	vitro	910:914	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	3	35	theme	weight-average	424:437	arg1	weight					449:454	weight-average molecular weight	424:454	weight-average molecular weight	424:454	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	1	36	theme	present	127:133	arg1	study					135:139	the present study	123:139	the present study	123:139	In the present study, a novel neutral polysaccharide SMP-0b was extracted and purified from the pulp of Solanum muricatum.
31712159	0	37	dep	Structure	0:8	arg1	characterization					10:25	characterization	10:25	characterization	10:25	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	7	38	dep	immunomodulator	1166:1180	arg1	an					1163:1164	an	1163:1164	an	1163:1164	The results suggested that the neutral polysaccharide from Solanum muricatum might have potential as an immunomodulator or supplement in functional food to enhance immunity.
31712159	1	39	theme	novel	144:148	arg1	SMP-0b					173:178	a novel neutral polysaccharide SMP-0b	142:178	a novel neutral polysaccharide SMP-0b	142:178	In the present study, a novel neutral polysaccharide SMP-0b was extracted and purified from the pulp of Solanum muricatum.
31712159	0	40	theme	polysaccharide	74:87	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	0	40	theme	polysaccharide	74:87	arg1	activity					48:55	immunomodulatory activity	31:55	immunomodulatory activity	31:55	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	6	41	theme	NO	1017:1018	arg1	production					1020:1029	NO production	1017:1029	NO production	1017:1029	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	2	42	theme	composition	258:268	arg1	analysis					270:277	Monosaccharide composition analysis	243:277	Monosaccharide composition analysis	243:277	Monosaccharide composition analysis revealed that SMP-0b was mainly composed of l-arabinose, d-mannose, d-glucose and d-galactose with the molar ratio of 5.31:2.92:42.23:25.38.
31712159	6	43	theme	RAW	1034:1036	arg1	cells					1055:1059	RAW 264.7 macrophage cells	1034:1059	RAW 264.7 macrophage cells	1034:1059	In immunomodulation assays in vitro, SMP-0b exhibited good immunomodulatory activity and could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells.
31712159	2	44	theme	molar	382:386	arg1	ratio					388:392	the molar ratio	378:392	the molar ratio of 5.31:2.92:42.23:25.38	378:417	Monosaccharide composition analysis revealed that SMP-0b was mainly composed of l-arabinose, d-mannose, d-glucose and d-galactose with the molar ratio of 5.31:2.92:42.23:25.38.
31712159	5	45	theme	α-l-Araf-	845:853	arg1	-α-d-Glcp-					865:874	α-l-Araf-(1→ and →4)-α-d-Glcp-(1→	845:877	α-l-Araf-(1→ and →4)-α-d-Glcp-(1→	845:877	It showed that the backbone chain of SMP-0b was consisted of →4)-β-d-Galp-(1→, →3,6)-β-d-Manp-(1→ and →6)-α-d-Glcp-(1→, and the side chain was composed of α-l-Araf-(1→ and →4)-α-d-Glcp-(1→.
31712159	1	46	theme	muricatum	232:240	arg1	pulp					216:219	the pulp	212:219	the pulp of Solanum muricatum	212:240	In the present study, a novel neutral polysaccharide SMP-0b was extracted and purified from the pulp of Solanum muricatum.
31712159	0	47	theme	neutral	66:72	arg1	polysaccharide					74:87	a new neutral polysaccharide	60:87	a new neutral polysaccharide	60:87	Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum.
31712159	2	48	theme	Monosaccharide	243:256	arg1	analysis					270:277	Monosaccharide composition analysis	243:277	Monosaccharide composition analysis	243:277	Monosaccharide composition analysis revealed that SMP-0b was mainly composed of l-arabinose, d-mannose, d-glucose and d-galactose with the molar ratio of 5.31:2.92:42.23:25.38.
31712159	3	49	theme	high	567:570	arg1	chromatography					599:612	high performance gel permeation chromatography	567:612	high performance gel permeation chromatography	567:612	The weight-average molecular weight and number-average molecular weight of SMP-0b was calculated to be 13.51 kDa and 9.91 kDa respectively through high performance gel permeation chromatography.
31712159	4	50	theme	NMR	676:678	arg1	analysis					680:687	NMR analysis	676:687	NMR analysis	676:687	The structure of SMP-0b was characterized by methylation and NMR analysis.
30423354	8	0	theme	mechanical	1130:1139	arg1	properties					1141:1150	the mechanical properties	1126:1150	the mechanical properties of DRCs except flexural modulus	1126:1182	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	9	1	theme	antibacterial	1236:1248	arg1	properties					1250:1259	excellent antibacterial properties	1226:1259	excellent antibacterial properties	1226:1259	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	6	2	contain	containing	869:878	arg2	2 wt					880:883	2 wt	880:883	2 wt	880:883	RESULTS Compared with DRCs without CNC/ZnO nanohybrids, DRCs containing 2 wt.
30423354	6	2	contain	containing	869:878	arg1	DRCs					864:867	DRCs	864:867	DRCs containing 2 wt	864:883	RESULTS Compared with DRCs without CNC/ZnO nanohybrids, DRCs containing 2 wt.
30423354	6	2	contain	containing	869:878	arg1	nanohybrids					851:861	CNC/ZnO nanohybrids	843:861	CNC/ZnO nanohybrids	843:861	RESULTS Compared with DRCs without CNC/ZnO nanohybrids, DRCs containing 2 wt.
30423354	7	3	contain	possess	907:913	arg2	modulus					956:962	flexural modulus	947:962	flexural modulus	947:962	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	3	contain	possess	907:913	arg2	strength					934:941	higher compressive strength	915:941	higher compressive strength	915:941	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	3	contain	possess	907:913	arg1	nanohybrids					895:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	1	4	theme	cellulose	178:186	arg1	nanohybrids					221:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	4	5	from	morphology	625:634	arg1	surface					667:673	the surface	663:673	the surface of DRCs after incubation	663:698	The antibacterial activity of DRCs to Streptococcus mutans was determined and the morphology of Streptococcus mutans on the surface of DRCs after incubation was observed.
30423354	10	6	theme	mechanical	1437:1446	arg1	properties					1466:1475	the mechanical and antibacterial properties	1433:1475	the mechanical and antibacterial properties of DRCs	1433:1483	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	6	7	theme	CNC/ZnO	843:849	arg1	DRCs					864:867	DRCs	864:867	DRCs containing 2 wt	864:883	RESULTS Compared with DRCs without CNC/ZnO nanohybrids, DRCs containing 2 wt.
30423354	6	7	theme	CNC/ZnO	843:849	arg1	nanohybrids					851:861	CNC/ZnO nanohybrids	843:861	CNC/ZnO nanohybrids	843:861	RESULTS Compared with DRCs without CNC/ZnO nanohybrids, DRCs containing 2 wt.
30423354	7	8	theme	higher	915:920	arg1	strength					934:941	higher compressive strength	915:941	higher compressive strength	915:941	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	8	9	theme	excess	1082:1087	arg1	use					1089:1091	The excess use	1078:1091	The excess use of CNC/ZnO nanohybrids	1078:1114	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	7	10	theme	statistical	994:1004	arg1	difference					1006:1015	no significantly statistical difference	977:1015	no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness	977:1075	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	10	theme	statistical	994:1004	arg1	P＞0.05					1018:1023	P＞0.05	1018:1023	P＞0.05	1018:1023	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	11	theme	CNC/ZnO	887:893	arg1	nanohybrids					895:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	11	12	theme	prepared	1503:1510	arg1	DRCs					1512:1515	The prepared DRCs	1499:1515	SIGNIFICANCE The prepared DRCs	1486:1515	SIGNIFICANCE The prepared DRCs are promising to address bulk fracture and secondary caries.
30423354	5	13	theme	Zn	774:775	arg1	content					777:783	flexural test and Zn content	756:783	flexural test and Zn content in DRCs	756:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	8	14	theme	CNC/ZnO	1096:1102	arg1	nanohybrids					1104:1114	CNC/ZnO nanohybrids	1096:1114	CNC/ZnO nanohybrids	1096:1114	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	1	15	from	effect	168:173	arg1	DRCs					261:264	DRCs	261:264	DRCs	261:264	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	1	15	from	effect	168:173	arg1	composites					249:258	dental resin composites	236:258	dental resin composites (DRCs)	236:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	1	16	theme	nanocrystal/zinc	188:203	arg1	nanohybrids					221:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	9	17	theme	%	1269:1269	arg1	reduction					1271:1279	a 78% reduction	1265:1279	a 78% reduction in bacterial number	1265:1299	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	3	18	theme	mechanical	398:407	arg1	properties					409:418	The mechanical properties	394:418	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness	394:521	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	10	19	theme	positive	1411:1418	arg1	influence					1420:1428	a positive influence	1409:1428	a positive influence	1409:1428	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	1	20	theme	oxide	205:209	arg1	nanohybrids					221:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	0	21	theme	antibacterial	7:19	arg1	composites					34:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	7	22	theme	%	885:885	arg1	nanohybrids					895:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids	885:905	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	3	23	theme	compressive	438:448	arg1	strength					450:457	compressive strength	438:457	compressive strength	438:457	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	0	24	theme	Strong	0:5	arg1	composites					34:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	8	25	theme	nanohybrids	1104:1114	arg1	use					1089:1091	The excess use	1078:1091	The excess use of CNC/ZnO nanohybrids	1078:1114	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	10	26	theme	DRCs	1480:1483	arg1	properties					1466:1475	the mechanical and antibacterial properties	1433:1475	the mechanical and antibacterial properties of DRCs	1433:1483	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	5	27	theme	test	765:768	arg1	content					777:783	flexural test and Zn content	756:783	flexural test and Zn content in DRCs	756:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	1	28	theme	CNC/ZnO	212:218	arg1	nanohybrids					221:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids	178:231	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	0	29	theme	resin	28:32	arg1	composites					34:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	9	30	contain	containing	1190:1199	arg1	DRCs					1185:1188	DRCs	1185:1188	DRCs containing CNC/ZnO nanohybrids	1185:1219	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	9	30	contain	containing	1190:1199	arg2	nanohybrids					1209:1219	CNC/ZnO nanohybrids	1201:1219	CNC/ZnO nanohybrids	1201:1219	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	5	31	theme	fractured	732:740	arg1	surface					742:748	fractured surface	732:748	fractured surface after flexural test and Zn content in DRCs	732:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	9	32	theme	bacterial	1284:1292	arg1	number					1294:1299	bacterial number	1284:1299	bacterial number	1284:1299	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	11	33	theme	bulk	1542:1545	arg1	fracture					1547:1554	bulk fracture	1542:1554	bulk fracture	1542:1554	SIGNIFICANCE The prepared DRCs are promising to address bulk fracture and secondary caries.
30423354	3	34	theme	flexural	478:485	arg1	strength					487:494	flexural strength	478:494	flexural strength	478:494	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	0	35	theme	dental	21:26	arg1	composites					34:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites	0:43	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	10	36	contain	have	1404:1407	arg2	influence					1420:1428	a positive influence	1409:1428	a positive influence	1409:1428	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	10	36	contain	have	1404:1407	arg1	amounts					1373:1379	The small amounts	1363:1379	The small amounts of CNC/ZnO nanohybrids	1363:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	10	36	contain	have	1404:1407	arg1	nanohybrids					1392:1402	CNC/ZnO nanohybrids	1384:1402	CNC/ZnO nanohybrids	1384:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	5	37	theme	surface	742:748	arg1	morphology					718:727	The morphology	714:727	The morphology of fractured surface after flexural test and Zn content in DRCs	714:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	10	38	theme	small	1367:1371	arg1	amounts					1373:1379	The small amounts	1363:1379	The small amounts of CNC/ZnO nanohybrids	1363:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	10	38	theme	small	1367:1371	arg1	nanohybrids					1392:1402	CNC/ZnO nanohybrids	1384:1402	CNC/ZnO nanohybrids	1384:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	1	39	theme	nanohybrids	221:231	arg1	effect					168:173	the reinforcing and antibacterial effect	134:173	the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs)	134:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	3	40	theme	DRCs	423:426	arg1	properties					409:418	The mechanical properties	394:418	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness	394:521	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	2	41	theme	CNC	355:357	arg1	surface					344:350	the surface	340:350	the surface of CNC	340:357	METHODS CNC/ZnO nanohybrids were prepared through precipitating Zn2+ on the surface of CNC and then introduced into the DRCs.
30423354	5	42	from	content	777:783	arg1	DRCs					788:791	DRCs	788:791	DRCs	788:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	2	43	dep	METHODS	268:274	arg1	prepared					301:308	prepared	301:308	prepared through precipitating Zn2+ on the surface of CNC	301:357	METHODS CNC/ZnO nanohybrids were prepared through precipitating Zn2+ on the surface of CNC and then introduced into the DRCs.
30423354	2	43	dep	METHODS	268:274	arg1	introduced					368:377	introduced	368:377	then introduced into the DRCs	363:391	METHODS CNC/ZnO nanohybrids were prepared through precipitating Zn2+ on the surface of CNC and then introduced into the DRCs.
30423354	4	44	theme	DRCs	678:681	arg1	surface					667:673	the surface	663:673	the surface of DRCs after incubation	663:698	The antibacterial activity of DRCs to Streptococcus mutans was determined and the morphology of Streptococcus mutans on the surface of DRCs after incubation was observed.
30423354	5	45	theme	flexural	756:763	arg1	content					777:783	flexural test and Zn content	756:783	flexural test and Zn content in DRCs	756:791	The morphology of fractured surface after flexural test and Zn content in DRCs were analyzed.
30423354	7	46	theme	Vickers	1055:1061	arg1	microhardness					1063:1075	Vickers microhardness	1055:1075	Vickers microhardness	1055:1075	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	1	47	theme	dental	236:241	arg1	DRCs					261:264	DRCs	261:264	DRCs	261:264	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	1	47	theme	dental	236:241	arg1	composites					249:258	dental resin composites	236:258	dental resin composites (DRCs)	236:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	0	48	theme	nanocrystal/zinc	66:81	arg1	nanohybrids					89:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	4	49	theme	antibacterial	547:559	arg1	activity					561:568	The antibacterial activity	543:568	The antibacterial activity of DRCs to Streptococcus mutans	543:600	The antibacterial activity of DRCs to Streptococcus mutans was determined and the morphology of Streptococcus mutans on the surface of DRCs after incubation was observed.
30423354	10	50	theme	antibacterial	1452:1464	arg1	properties					1466:1475	the mechanical and antibacterial properties	1433:1475	the mechanical and antibacterial properties of DRCs	1433:1483	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	3	51	theme	Vickers	501:507	arg1	microhardness					509:521	Vickers microhardness	501:521	Vickers microhardness	501:521	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	0	52	theme	cellulose	56:64	arg1	nanohybrids					89:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	4	53	theme	DRCs	573:576	arg1	activity					561:568	The antibacterial activity	543:568	The antibacterial activity of DRCs to Streptococcus mutans	543:600	The antibacterial activity of DRCs to Streptococcus mutans was determined and the morphology of Streptococcus mutans on the surface of DRCs after incubation was observed.
30423354	8	54	theme	DRCs	1155:1158	arg1	properties					1141:1150	the mechanical properties	1126:1150	the mechanical properties of DRCs except flexural modulus	1126:1182	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	9	55	theme	2	1318:1318	arg1	%					1319:1319	%	1319:1319	%	1319:1319	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	10	56	theme	nanohybrids	1392:1402	arg1	amounts					1373:1379	The small amounts	1363:1379	The small amounts of CNC/ZnO nanohybrids	1363:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	10	56	theme	nanohybrids	1392:1402	arg1	nanohybrids					1392:1402	CNC/ZnO nanohybrids	1384:1402	CNC/ZnO nanohybrids	1384:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	1	57	dep	OBJECTIVE	102:110	arg1	is					120:121	is	120:121	is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs)	120:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	11	58	theme	secondary	1560:1568	arg1	caries					1570:1575	secondary caries	1560:1575	secondary caries	1560:1575	SIGNIFICANCE The prepared DRCs are promising to address bulk fracture and secondary caries.
30423354	9	59	theme	%	1319:1319	arg1	nanohybrids					1329:1339	2% CNC/ZnO nanohybrids	1318:1339	2% CNC/ZnO nanohybrids	1318:1339	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	2	60	theme	CNC/ZnO	276:282	arg1	nanohybrids					284:294	CNC/ZnO nanohybrids	276:294	CNC/ZnO nanohybrids	276:294	METHODS CNC/ZnO nanohybrids were prepared through precipitating Zn2+ on the surface of CNC and then introduced into the DRCs.
30423354	9	61	theme	CNC/ZnO	1201:1207	arg1	nanohybrids					1209:1219	CNC/ZnO nanohybrids	1201:1219	CNC/ZnO nanohybrids	1201:1219	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	1	62	theme	reinforcing	138:148	arg1	effect					168:173	the reinforcing and antibacterial effect	134:173	the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs)	134:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	10	63	dep	CONCLUSION	1352:1361	arg1	have					1404:1407	have	1404:1407	have a positive influence on the mechanical and antibacterial properties of DRCs	1404:1483	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	1	64	theme	resin	243:247	arg1	DRCs					261:264	DRCs	261:264	DRCs	261:264	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	1	64	theme	resin	243:247	arg1	composites					249:258	dental resin composites	236:258	dental resin composites (DRCs)	236:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	8	65	theme	flexural	1167:1174	arg1	modulus					1176:1182	flexural modulus	1167:1182	flexural modulus	1167:1182	The excess use of CNC/ZnO nanohybrids decreases the mechanical properties of DRCs except flexural modulus.
30423354	9	66	theme	CNC/ZnO	1321:1327	arg1	nanohybrids					1329:1339	2% CNC/ZnO nanohybrids	1318:1339	2% CNC/ZnO nanohybrids	1318:1339	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	9	67	from	reduction	1271:1279	arg1	number					1294:1299	bacterial number	1284:1299	bacterial number	1284:1299	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	10	68	theme	CNC/ZnO	1384:1390	arg1	nanohybrids					1392:1402	CNC/ZnO nanohybrids	1384:1402	CNC/ZnO nanohybrids	1384:1402	CONCLUSION The small amounts of CNC/ZnO nanohybrids have a positive influence on the mechanical and antibacterial properties of DRCs.
30423354	7	69	theme	flexural	947:954	arg1	modulus					956:962	flexural modulus	947:962	flexural modulus	947:962	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	70	theme	flexural	1033:1040	arg1	strength					1042:1049	the flexural strength	1029:1049	the flexural strength	1029:1049	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	1	71	theme	antibacterial	154:166	arg1	effect					168:173	the reinforcing and antibacterial effect	134:173	the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs)	134:265	OBJECTIVE The aim is to explore the reinforcing and antibacterial effect of cellulose nanocrystal/zinc oxide (CNC/ZnO) nanohybrids on dental resin composites (DRCs).
30423354	3	72	theme	flexural	460:467	arg1	modulus					469:475	flexural modulus	460:475	flexural modulus	460:475	The mechanical properties of DRCs including compressive strength, flexural modulus, flexural strength, and Vickers microhardness were characterized.
30423354	11	73	dep	SIGNIFICANCE	1486:1497	arg1	DRCs					1512:1515	The prepared DRCs	1499:1515	SIGNIFICANCE The prepared DRCs	1486:1515	SIGNIFICANCE The prepared DRCs are promising to address bulk fracture and secondary caries.
30423354	0	74	theme	oxide	83:87	arg1	nanohybrids					89:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	cellulose nanocrystal/zinc oxide nanohybrids	56:99	Strong antibacterial dental resin composites containing cellulose nanocrystal/zinc oxide nanohybrids.
30423354	9	75	theme	excellent	1226:1234	arg1	properties					1250:1259	excellent antibacterial properties	1226:1259	excellent antibacterial properties	1226:1259	DRCs containing CNC/ZnO nanohybrids show excellent antibacterial properties and a 78% reduction in bacterial number is obtained when 2% CNC/ZnO nanohybrids are added.
30423354	7	76	from	difference	1006:1015	arg1	strength					1042:1049	the flexural strength	1029:1049	the flexural strength	1029:1049	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	76	from	difference	1006:1015	arg1	microhardness					1063:1075	Vickers microhardness	1055:1075	Vickers microhardness	1055:1075	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	7	77	theme	compressive	922:932	arg1	strength					934:941	higher compressive strength	915:941	higher compressive strength	915:941	% CNC/ZnO nanohybrids possess higher compressive strength and flexural modulus and there is no significantly statistical difference (P＞0.05) on the flexural strength and Vickers microhardness.
30423354	4	78	theme	mutans	653:658	arg1	morphology					625:634	the morphology	621:634	the morphology of Streptococcus mutans on the surface of DRCs after incubation	621:698	The antibacterial activity of DRCs to Streptococcus mutans was determined and the morphology of Streptococcus mutans on the surface of DRCs after incubation was observed.
31655418	3	0	theme	1H	626:627	arg1	analysis					641:648	1H and 13C NMR analysis	626:648	1H and 13C NMR analysis	626:648	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	6	1	theme	oligosaccharides	1162:1177	arg1	production					1096:1105	production	1096:1105	production	1096:1105	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	6	1	theme	oligosaccharides	1162:1177	arg1	characterisation					1122:1137	functional characterisation	1111:1137	functional characterisation	1111:1137	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	3	2	theme	1 → 6	574:578	arg1	linkages					581:588	α-(1 → 6) linkages	571:588	α-(1 → 6) linkages	571:588	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	1	3	theme	donor-acceptor	309:322	arg1	sugars					324:329	donor-acceptor sugars	309:329	donor-acceptor sugars	309:329	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	3	theme	donor-acceptor	309:322	arg1	oligosaccharides					157:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	0	4	theme	prebiotic	94:102	arg1	functions					126:134	prebiotic and immune-modulatory functions	94:134	prebiotic and immune-modulatory functions	94:134	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	5	5	theme	IL-4	1001:1004	arg1	cytokines					1022:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	6	6	theme	future	1215:1220	arg1	applications					1222:1233	future applications	1215:1233	future applications	1215:1233	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	3	7	link	gentiobiose-derived	684:702	arg1	oligosaccharides					704:719	these gentiobiose-derived oligosaccharides	678:719	these gentiobiose-derived oligosaccharides	678:719	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	4	8	located	observed	858:865	arg2	growth					847:852	no growth	844:852	no growth	844:852	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	4	8	located	observed	858:865	arg1	strains					879:885	pathogen strains	870:885	pathogen strains	870:885	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	0	9	theme	functions	126:134	arg1	determination					77:89	determination	77:89	determination	77:89	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	0	9	theme	functions	126:134	arg1	E81					69:71	glucansucrase E81	55:71	glucansucrase E81	55:71	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	2	10	link	gentiobiose-derived	403:421	arg1	oligosaccharides					423:438	gentiobiose-derived oligosaccharides	403:438	gentiobiose-derived oligosaccharides up to DP 8	403:449	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	0	11	link	gentiobiose-derived	15:33	arg1	oligosaccharides					35:50	gentiobiose-derived oligosaccharides	15:50	gentiobiose-derived oligosaccharides	15:50	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	3	12	theme	1 → 3	597:601	arg1	linkages					604:611	α-(1 → 3) linkages	594:611	α-(1 → 3) linkages	594:611	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	0	13	theme	immune-modulatory	108:124	arg1	functions					126:134	prebiotic and immune-modulatory functions	94:134	prebiotic and immune-modulatory functions	94:134	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	6	14	link	gentiobiose-derived	1142:1160	arg1	oligosaccharides					1162:1177	gentiobiose-derived oligosaccharides	1142:1177	gentiobiose-derived oligosaccharides establishing a promising avenue for future applications	1142:1233	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	5	15	theme	dependent	1056:1064	arg1	manner					1066:1071	a dose dependent manner	1049:1071	a dose dependent manner	1049:1071	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	5	16	from	production	987:996	arg1	HT29 cells					1035:1044	HT29 cells	1035:1044	HT29 cells	1035:1044	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	2	17	theme	reaction	350:357	arg1	products					359:366	The reaction products	346:366	The reaction products	346:366	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	5	18	theme	cytokines	1022:1030	arg1	production					987:996	the production	983:996	the production of IL-4, IL12 and TNF-α cytokines in HT29 cells	983:1044	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	5	19	theme	IL12	1007:1010	arg1	cytokines					1022:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	3	20	with	gentiobiose	554:564	arg1	linkages					581:588	α-(1 → 6) linkages	571:588	α-(1 → 6) linkages	571:588	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	3	20	with	gentiobiose	554:564	arg1	linkages					604:611	α-(1 → 3) linkages	594:611	α-(1 → 3) linkages	594:611	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	4	21	theme	probiotic	802:810	arg1	strains					812:818	probiotic strains	802:818	probiotic strains	802:818	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	3	22	theme	α-	594:595	arg1	linkages					604:611	α-(1 → 3) linkages	594:611	α-(1 → 3) linkages	594:611	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	1	23	theme	glucansucrase	219:231	arg1	E81					233:235	glucansucrase E81	219:235	glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose	219:304	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	2	24	dep	DP	446:447	arg1	up					440:441	up	440:441	up	440:441	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	1	25	theme	E81	233:235	arg1	reaction					207:214	the acceptor reaction	194:214	the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose	194:304	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	2	26	theme	TLC	386:388	arg1	analysis					390:397	TLC analysis	386:397	TLC analysis	386:397	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	6	27	dep	production	1096:1105	arg1	the					1092:1094	the	1092:1094	the	1092:1094	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	6	28	theme	promising	1194:1202	arg1	avenue					1204:1209	a promising avenue	1192:1209	a promising avenue for future applications	1192:1233	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	6	29	theme	functional	1111:1120	arg1	characterisation					1122:1137	functional characterisation	1111:1137	functional characterisation	1111:1137	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	4	30	theme	in	726:727	arg1	function					745:752	The in vitro prebiotic function	722:752	The in vitro prebiotic function of the oligosaccharides	722:776	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	0	31	theme	oligosaccharides	35:50	arg1	Preparation					0:10	Preparation	0:10	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.	0:135	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	4	32	theme	pathogen	870:877	arg1	strains					879:885	pathogen strains	870:885	pathogen strains	870:885	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	1	33	with	E81	273:275	arg1	gentiobiose					294:304	gentiobiose	294:304	gentiobiose	294:304	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	33	with	E81	273:275	arg1	sucrose					282:288	sucrose	282:288	sucrose	282:288	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	34	theme	Lactobacillus	251:263	arg1	E81					273:275	Lactobacillus reuteri E81	251:275	Lactobacillus reuteri E81 with sucrose and gentiobiose	251:304	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	0	35	theme	gentiobiose-derived	15:33	arg1	oligosaccharides					35:50	gentiobiose-derived oligosaccharides	15:50	gentiobiose-derived oligosaccharides	15:50	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	3	36	theme	gentiobiose-derived	684:702	arg1	oligosaccharides					704:719	these gentiobiose-derived oligosaccharides	678:719	these gentiobiose-derived oligosaccharides	678:719	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	1	37	theme	Gentiobiose-derived	137:155	arg1	sugars					324:329	donor-acceptor sugars	309:329	donor-acceptor sugars	309:329	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	37	theme	Gentiobiose-derived	137:155	arg1	oligosaccharides					157:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	5	38	theme	immune-modulatory	932:948	arg1	functions					950:958	immune-modulatory functions	932:958	immune-modulatory functions	932:958	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	0	39	theme	glucansucrase	55:67	arg1	E81					69:71	glucansucrase E81	55:71	glucansucrase E81	55:71	Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions.
31655418	4	40	theme	oligosaccharides	761:776	arg1	function					745:752	The in vitro prebiotic function	722:752	The in vitro prebiotic function of the oligosaccharides	722:776	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	3	41	theme	oligosaccharides	704:719	arg1	structure					665:673	the structure	661:673	the structure of these gentiobiose-derived oligosaccharides	661:719	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	3	42	theme	13C	633:635	arg1	NMR					637:639	13C NMR	633:639	13C NMR	633:639	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	6	43	theme	gentiobiose-derived	1142:1160	arg1	oligosaccharides					1162:1177	gentiobiose-derived oligosaccharides	1142:1177	gentiobiose-derived oligosaccharides establishing a promising avenue for future applications	1142:1233	This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
31655418	2	44	theme	ESI-MS/MS	509:517	arg1	analysis					519:526	ESI-MS/MS analysis	509:526	ESI-MS/MS analysis	509:526	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	2	45	theme	acceptor	474:481	arg1	reaction					483:490	the acceptor reaction	470:490	the acceptor reaction	470:490	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	3	46	gly	glycosylation	533:545	arg1	gentiobiose					554:564	the gentiobiose	550:564	the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages	550:611	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	3	47	theme	α-	571:572	arg1	linkages					581:588	α-(1 → 6) linkages	571:588	α-(1 → 6) linkages	571:588	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	5	48	theme	Gentiobiose-derived	888:906	arg1	oligosaccharides					908:923	Gentiobiose-derived oligosaccharides	888:923	Gentiobiose-derived oligosaccharides	888:923	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	2	49	theme	gentiobiose-derived	403:421	arg1	oligosaccharides					423:438	gentiobiose-derived oligosaccharides	403:438	gentiobiose-derived oligosaccharides up to DP 8	403:449	The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis.
31655418	5	50	link	Gentiobiose-derived	888:906	arg1	oligosaccharides					908:923	Gentiobiose-derived oligosaccharides	888:923	Gentiobiose-derived oligosaccharides	888:923	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	3	51	theme	NMR	637:639	arg1	analysis					641:648	1H and 13C NMR analysis	626:648	1H and 13C NMR analysis	626:648	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	3	52	theme	gentiobiose	554:564	arg1	glycosylation					533:545	The glycosylation	529:545	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages	529:611	The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides.
31655418	4	53	dep	in	726:727	arg1	vitro					729:733	vitro	729:733	vitro	729:733	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	4	54	theme	prebiotic	735:743	arg1	function					745:752	The in vitro prebiotic function	722:752	The in vitro prebiotic function of the oligosaccharides	722:776	The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains.
31655418	5	55	theme	TNF-α	1016:1020	arg1	cytokines					1022:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	IL-4, IL12 and TNF-α cytokines	1001:1030	Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner.
31655418	1	56	dep	Lactobacillus	251:263	arg1	reuteri					265:271	reuteri	265:271	reuteri	265:271	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	57	link	Gentiobiose-derived	137:155	arg1	sugars					324:329	donor-acceptor sugars	309:329	donor-acceptor sugars	309:329	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	57	link	Gentiobiose-derived	137:155	arg1	oligosaccharides					157:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides	137:172	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
31655418	1	58	theme	acceptor	198:205	arg1	reaction					207:214	the acceptor reaction	194:214	the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose	194:304	Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively.
29392382	7	0	theme	functional	1389:1398	arg1	measurements					1410:1421	functional diversity measurements	1389:1421	functional diversity measurements	1389:1421	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	7	1	theme	cellulose	1620:1628	arg1	degradation					1648:1658	cellulose and hemicellulose degradation	1620:1658	cellulose and hemicellulose degradation	1620:1658	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	1	2	theme	microbial	102:110	arg1	communities					112:122	Enriched microbial communities	93:122	Enriched microbial communities	93:122	Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation.
29392382	3	3	theme	industrial	618:627	arg1	processes					629:637	more controlled industrial processes	602:637	more controlled industrial processes	602:637	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	4	4	theme	active	759:764	arg1	MAMC					787:790	MAMC	787:790	MAMC	787:790	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	4	4	theme	active	759:764	arg1	Consortia					776:784	an effective lignocellulose-degrading minimal active microbial Consortia	713:784	an effective lignocellulose-degrading minimal active microbial Consortia (MAMC)	713:791	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	6	5	theme	capacity	1300:1307	arg1	number					1234:1239	the number	1230:1239	the number of functional groups, metabolic potential, and degradation capacity	1230:1307	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	1	6	theme	lignocellulose	220:233	arg1	degradation					235:245	lignocellulose degradation	220:245	lignocellulose degradation	220:245	Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation.
29392382	2	7	theme	dynamic	360:366	arg1	nature					368:373	intrinsically dynamic nature	346:373	intrinsically dynamic nature	346:373	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	4	8	theme	lignocellulose-degrading	726:749	arg1	MAMC					787:790	MAMC	787:790	MAMC	787:790	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	4	8	theme	lignocellulose-degrading	726:749	arg1	Consortia					776:784	an effective lignocellulose-degrading minimal active microbial Consortia	713:784	an effective lignocellulose-degrading minimal active microbial Consortia (MAMC)	713:791	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	2	9	theme	industrial	280:289	arg1	processes					303:311	fully controlled industrial degradation processes	263:311	fully controlled industrial degradation processes	263:311	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	5	10	theme	reductive-screening	916:934	arg1	approach					936:943	a reductive-screening approach	914:943	a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization	914:1022	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	2	11	dep	nature	368:373	arg1	the					342:344	the	342:344	the	342:344	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	1	12	theme	environmental	139:151	arg1	samples					153:159	environmental samples	139:159	environmental samples	139:159	Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation.
29392382	9	13	theme	assembled	1934:1942	arg1	communities					1954:1964	seven assembled synthetic communities	1928:1964	seven assembled synthetic communities	1928:1964	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	7	14	theme	lignocellulosic	1521:1535	arg1	lignin					1547:1552	lignin	1547:1552	lignin	1547:1552	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	7	14	theme	lignocellulosic	1521:1535	arg1	fraction					1537:1544	the most complex lignocellulosic fraction	1504:1544	the most complex lignocellulosic fraction (lignin)	1504:1553	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	5	15	theme	lignocellulose	856:869	arg1	material					871:878	lignocellulose material	856:878	lignocellulose material from sugarcane origin	856:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	11	16	theme	degrading	2189:2197	arg1	community					2209:2217	the most effective degrading synthetic community	2170:2217	the most effective degrading synthetic community	2170:2217	was found to be the most effective degrading synthetic community.
29392382	9	17	theme	communities	1954:1964	arg1	total					1919:1923	A total	1917:1923	A total of seven assembled synthetic communities	1917:1964	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	9	17	theme	communities	1954:1964	arg1	MAMC					2004:2007	the most effective MAMC	1985:2007	the most effective MAMC	1985:2007	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	7	18	theme	degradation	1362:1372	arg1	capacities					1374:1383	degradation capacities	1362:1383	degradation capacities	1362:1383	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	5	19	theme	bacteria	826:833	arg1	enrichment					807:816	an enrichment	804:816	an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin	804:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	5	20	theme	functional	1113:1122	arg1	groups					1124:1129	four metabolic functional groups	1098:1129	four metabolic functional groups	1098:1129	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	6	21	dep	compositional	1153:1165	arg1	replicates					1167:1176	replicates	1167:1176	replicates	1167:1176	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	2	22	theme	species	478:484	arg1	number					440:445	a large number	432:445	a large number of culturable and unculturable species	432:484	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	2	23	theme	culturable	450:459	arg1	species					478:484	culturable and unculturable species	450:484	culturable and unculturable species	450:484	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	7	24	theme	MAMC	1338:1341	arg1	characterization					1314:1329	The characterization	1310:1329	The characterization of the MAMC according to their degradation capacities and functional diversity measurements	1310:1421	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	0	25	theme	Microbial	41:49	arg1	Consortia					51:59	Effective Minimal Active Microbial Consortia	16:59	Effective Minimal Active Microbial Consortia for Lignocellulose Degradation	16:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	1	26	theme	Enriched	93:100	arg1	communities					112:122	Enriched microbial communities	93:122	Enriched microbial communities	93:122	Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation.
29392382	7	27	theme	complex	1513:1519	arg1	lignin					1547:1552	lignin	1547:1552	lignin	1547:1552	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	7	27	theme	complex	1513:1519	arg1	fraction					1537:1544	the most complex lignocellulosic fraction	1504:1544	the most complex lignocellulosic fraction (lignin)	1504:1553	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	9	28	theme	effective	1994:2002	arg1	total					1919:1923	A total	1917:1923	A total of seven assembled synthetic communities	1917:1964	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	9	28	theme	effective	1994:2002	arg1	MAMC					2004:2007	the most effective MAMC	1985:2007	the most effective MAMC	1985:2007	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	5	29	theme	strains	1077:1083	arg1	selection					1036:1044	a selection	1034:1044	a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups	1034:1129	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	0	30	theme	Effective	16:24	arg1	Consortia					51:59	Effective Minimal Active Microbial Consortia	16:59	Effective Minimal Active Microbial Consortia for Lignocellulose Degradation	16:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	7	31	dep	revealed	1423:1430	arg1	whereas					1612:1618	whereas	1612:1618	whereas	1612:1618	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	1	32	theme	selective	169:177	arg1	processes					179:187	selective processes	169:187	selective processes	169:187	Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation.
29392382	2	33	theme	unculturable	465:476	arg1	species					478:484	culturable and unculturable species	450:484	culturable and unculturable species	450:484	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	3	34	theme	complex	503:509	arg1	consortia					543:551	less complex but equally effective microbial consortia	498:551	less complex but equally effective microbial consortia	498:551	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	0	35	theme	Active	34:39	arg1	Consortia					51:59	Effective Minimal Active Microbial Consortia	16:59	Effective Minimal Active Microbial Consortia for Lignocellulose Degradation	16:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	0	36	theme	Lignocellulose	65:78	arg1	Degradation					80:90	Lignocellulose Degradation	65:90	Lignocellulose Degradation	65:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	2	37	theme	communities	407:417	arg1	nature					368:373	intrinsically dynamic nature	346:373	intrinsically dynamic nature	346:373	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	2	37	theme	communities	407:417	arg1	complexity					379:388	complexity	379:388	complexity	379:388	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	0	38	theme	Consortia	51:59	arg1	Construction					0:11	Construction	0:11	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation	0:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	6	39	theme	metabolic	1263:1271	arg1	potential					1273:1281	metabolic potential	1263:1281	metabolic potential	1263:1281	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	3	40	theme	microbial	533:541	arg1	consortia					543:551	less complex but equally effective microbial consortia	498:551	less complex but equally effective microbial consortia	498:551	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	7	41	theme	functional	1437:1446	arg1	diversity					1448:1456	functional diversity	1437:1456	functional diversity	1437:1456	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	8	42	theme	MAMC	1807:1810	arg1	selection					1784:1792	the selection	1780:1792	the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present	1780:1914	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	8	43	theme	rates	1877:1881	arg1	%					1856:1856	up 96.5%	1849:1856	up 96.5% of the degradation rates	1849:1881	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	8	43	theme	rates	1877:1881	arg1	rates					1877:1881	the degradation rates	1861:1881	the degradation rates	1861:1881	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	7	44	theme	diversity	1400:1408	arg1	measurements					1410:1421	functional diversity measurements	1389:1421	functional diversity measurements	1389:1421	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	3	45	theme	controlled	607:616	arg1	processes					629:637	more controlled industrial processes	602:637	more controlled industrial processes	602:637	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	7	46	theme	metabolic	1585:1593	arg1	complementarity					1595:1609	metabolic complementarity	1585:1609	metabolic complementarity	1585:1609	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	4	47	theme	ecological	658:667	arg1	theory					669:674	ecological theory	658:674	ecological theory	658:674	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	7	48	theme	hemicellulose	1634:1646	arg1	degradation					1648:1658	cellulose and hemicellulose degradation	1620:1658	cellulose and hemicellulose degradation	1620:1658	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	4	49	theme	minimal	751:757	arg1	MAMC					787:790	MAMC	787:790	MAMC	787:790	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	4	49	theme	minimal	751:757	arg1	Consortia					776:784	an effective lignocellulose-degrading minimal active microbial Consortia	713:784	an effective lignocellulose-degrading minimal active microbial Consortia (MAMC)	713:791	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	7	50	theme	complementarity	1595:1609	arg1	importance					1571:1580	the importance	1567:1580	the importance of metabolic complementarity	1567:1609	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	4	51	theme	microbial	766:774	arg1	MAMC					787:790	MAMC	787:790	MAMC	787:790	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	4	51	theme	microbial	766:774	arg1	Consortia					776:784	an effective lignocellulose-degrading minimal active microbial Consortia	713:784	an effective lignocellulose-degrading minimal active microbial Consortia (MAMC)	713:791	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	6	52	theme	degradation	1288:1298	arg1	capacity					1300:1307	degradation capacity	1288:1307	degradation capacity	1288:1307	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	2	53	theme	degradation	291:301	arg1	processes					303:311	fully controlled industrial degradation processes	263:311	fully controlled industrial degradation processes	263:311	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	5	54	theme	molecular	954:962	arg1	phenotyping					964:974	molecular phenotyping	954:974	molecular phenotyping	954:974	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	4	55	theme	effective	716:724	arg1	MAMC					787:790	MAMC	787:790	MAMC	787:790	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	4	55	theme	effective	716:724	arg1	Consortia					776:784	an effective lignocellulose-degrading minimal active microbial Consortia	713:784	an effective lignocellulose-degrading minimal active microbial Consortia (MAMC)	713:791	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	2	56	theme	controlled	269:278	arg1	processes					303:311	fully controlled industrial degradation processes	263:311	fully controlled industrial degradation processes	263:311	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	11	57	theme	synthetic	2199:2207	arg1	community					2209:2217	the most effective degrading synthetic community	2170:2217	the most effective degrading synthetic community	2170:2217	was found to be the most effective degrading synthetic community.
29392382	6	58	theme	MAMC	1181:1184	arg1	compositional					1153:1165	compositional	1153:1165	compositional	1153:1165	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	11	59	theme	effective	2179:2187	arg1	community					2209:2217	the most effective degrading synthetic community	2170:2217	the most effective degrading synthetic community	2170:2217	was found to be the most effective degrading synthetic community.
29392382	5	60	theme	metabolic	997:1005	arg1	characterization					1007:1022	metabolic characterization	997:1022	metabolic characterization	997:1022	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	9	61	theme	synthetic	1944:1952	arg1	communities					1954:1964	seven assembled synthetic communities	1928:1964	seven assembled synthetic communities	1928:1964	A total of seven assembled synthetic communities were identified as the most effective MAMC.
29392382	7	62	theme	fraction	1537:1544	arg1	degradation					1489:1499	the degradation	1485:1499	the degradation of the most complex lignocellulosic fraction (lignin)	1485:1553	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	8	63	theme	screening	1728:1736	arg1	method					1738:1743	The screening method	1724:1743	The screening method described here	1724:1758	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	5	64	theme	soil	821:824	arg1	bacteria					826:833	soil bacteria	821:833	soil bacteria capable of degrading lignocellulose material from sugarcane origin	821:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	5	65	from	origin	895:900	arg1	material					871:878	lignocellulose material	856:878	lignocellulose material from sugarcane origin	856:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	5	66	theme	metabolic	1103:1111	arg1	groups					1124:1129	four metabolic functional groups	1098:1129	four metabolic functional groups	1098:1129	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	6	67	contain	containing	1186:1195	arg2	species					1202:1208	five species	1197:1208	five species each	1197:1213	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	6	67	contain	containing	1186:1195	arg1	MAMC					1181:1184	MAMC	1181:1184	MAMC	1181:1184	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	6	68	theme	groups	1255:1260	arg1	number					1234:1239	the number	1230:1239	the number of functional groups, metabolic potential, and degradation capacity	1230:1307	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	5	69	theme	capable	835:841	arg1	bacteria					826:833	soil bacteria	821:833	soil bacteria capable of degrading lignocellulose material from sugarcane origin	821:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	6	70	dep	species	1202:1208	arg1	each					1210:1213	each	1210:1213	each	1210:1213	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	5	71	theme	lignocellulose-degrading	1052:1075	arg1	strains					1077:1083	18 lignocellulose-degrading strains	1049:1083	18 lignocellulose-degrading strains representing four metabolic functional groups	1049:1129	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	2	72	theme	large	434:438	arg1	number					440:445	a large number	432:445	a large number of culturable and unculturable species	432:484	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	0	73	theme	Minimal	26:32	arg1	Consortia					51:59	Effective Minimal Active Microbial Consortia	16:59	Effective Minimal Active Microbial Consortia for Lignocellulose Degradation	16:90	Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation.
29392382	3	74	theme	effective	523:531	arg1	consortia					543:551	less complex but equally effective microbial consortia	498:551	less complex but equally effective microbial consortia	498:551	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	5	75	theme	sugarcane	885:893	arg1	origin					895:900	sugarcane origin	885:900	sugarcane origin	885:900	Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups.
29392382	10	76	theme	Paenibacillus	2064:2076	arg1	sp.					2078:2080	Paenibacillus sp.	2064:2080	Paenibacillus sp.	2064:2080	A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp.
29392382	6	77	theme	potential	1273:1281	arg1	number					1234:1239	the number	1230:1239	the number of functional groups, metabolic potential, and degradation capacity	1230:1307	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	8	78	theme	effective	1797:1805	arg1	MAMC					1807:1810	effective MAMC	1797:1810	effective MAMC	1797:1810	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	2	79	theme	microbial	397:405	arg1	communities					407:417	the microbial communities	393:417	the microbial communities	393:417	Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species.
29392382	4	80	theme	enrichment	680:689	arg1	principles					691:700	enrichment principles	680:700	enrichment principles	680:700	Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC).
29392382	3	81	theme	consortia	543:551	arg1	use					491:493	The use	487:493	The use of less complex but equally effective microbial consortia	487:551	The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes.
29392382	8	82	theme	degradation	1865:1875	arg1	rates					1877:1881	the degradation rates	1861:1881	the degradation rates	1861:1881	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	7	83	theme	functional	1702:1711	arg1	diversity					1713:1721	functional diversity	1702:1721	functional diversity	1702:1721	The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity.
29392382	6	84	theme	functional	1244:1253	arg1	groups					1255:1260	functional groups	1244:1260	functional groups	1244:1260	We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity.
29392382	8	85	theme	degradation	1819:1829	arg1	potential					1831:1839	potential	1831:1839	potential	1831:1839	The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present.
29392382	10	86	theme	Brevundimonas	2136:2148	arg1	sp					2150:2151	Brevundimonas sp	2136:2151	Brevundimonas sp	2136:2151	A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp.
31324025	4	0	theme	carbohydrates	710:722	arg1	extracts					660:667	E2 extracts	657:667	E2 extracts	657:667	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	4	0	theme	carbohydrates	710:722	arg1	mixture					676:682	a mixture	674:682	a mixture of minerals, proteins and carbohydrates	674:722	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	0	1	from	Characterization	9:24	arg1	Posidonia					53:61	Posidonia	53:61	Posidonia	53:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	4	2	theme	structural	530:539	arg1	characterization					541:556	structural characterization	530:556	structural characterization	530:556	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	7	3	theme	waste	1121:1125	arg1	biomass					1127:1133	P. oceanica waste biomass	1109:1133	P. oceanica waste biomass	1109:1133	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	1	4	theme	oceanica	97:104	arg1	biomass					112:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated.
31324025	6	5	theme	E3	905:906	arg1	extracts					908:915	All E2 and E3 extracts	894:915	All E2 and E3 extracts	894:915	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	7	6	theme	biomass	1127:1133	arg1	potential					1096:1104	the potential	1092:1104	the potential of P. oceanica waste biomass for the production of bioactive extracts	1092:1174	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	6	7	theme	several	941:947	arg1	fungi					959:963	several foodborne fungi	941:963	several foodborne fungi	941:963	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	2	8	theme	water	316:320	arg1	methods					322:328	ultrasound-assisted and hot water methods	288:328	ultrasound-assisted and hot water methods	288:328	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	5	9	theme	proteins	864:871	arg1	presence					852:859	the presence	848:859	the presence of proteins and polysaccharides	848:891	All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides.
31324025	5	10	theme	high	760:763	arg1	capacity					777:784	remarkably high antioxidant capacity	749:784	remarkably high antioxidant capacity	749:784	All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides.
31324025	0	11	from	Posidonia	53:61	arg1	Characterization					9:24	In-Depth Characterization	0:24	In-Depth Characterization of Bioactive Extracts from Posidonia	0:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	0	11	from	Posidonia	53:61	arg1	Extracts					39:46	Bioactive Extracts	29:46	Bioactive Extracts from Posidonia	29:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	7	12	theme	P.	1109:1110	arg1	biomass					1127:1133	P. oceanica waste biomass	1109:1133	P. oceanica waste biomass	1109:1133	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	2	13	theme	hot	312:314	arg1	methods					322:328	ultrasound-assisted and hot water methods	288:328	ultrasound-assisted and hot water methods	288:328	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	4	14	theme	minerals	687:694	arg1	extracts					660:667	E2 extracts	657:667	E2 extracts	657:667	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	4	14	theme	minerals	687:694	arg1	mixture					676:682	a mixture	674:682	a mixture of minerals, proteins and carbohydrates	674:722	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	1	15	theme	different	171:179	arg1	methodologies					181:193	different methodologies	171:193	different methodologies	171:193	Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated.
31324025	5	16	theme	antioxidant	765:775	arg1	capacity					777:784	remarkably high antioxidant capacity	749:784	remarkably high antioxidant capacity	749:784	All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides.
31324025	6	17	theme	E2	898:899	arg1	extracts					908:915	All E2 and E3 extracts	894:915	All E2 and E3 extracts	894:915	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	7	18	theme	oceanica	1112:1119	arg1	biomass					1127:1133	P. oceanica waste biomass	1109:1133	P. oceanica waste biomass	1109:1133	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	1	19	theme	Posidonia	87:95	arg1	biomass					112:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated.
31324025	3	20	theme	organic	460:466	arg1	solvents					468:475	organic solvents	460:475	organic solvents	460:475	Moreover, a conventional protocol with organic solvents was applied, yielding E3 extracts.
31324025	5	21	theme	phenolic	817:824	arg1	compounds					826:834	phenolic compounds	817:834	phenolic compounds	817:834	All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides.
31324025	6	22	theme	E3	977:978	arg1	extracts					980:987	only E3 extracts	972:987	only E3 extracts	972:987	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	2	23	dep	ethanol-affinity	364:379	arg1	E1					382:383	E1	382:383	E1: ethanol soluble; E2: non-soluble	382:417	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	3	24	theme	conventional	433:444	arg1	protocol					446:453	a conventional protocol	431:453	a conventional protocol with organic solvents	431:475	Moreover, a conventional protocol with organic solvents was applied, yielding E3 extracts.
31324025	0	25	theme	In-Depth	0:7	arg1	Characterization					9:24	In-Depth Characterization	0:24	In-Depth Characterization of Bioactive Extracts from Posidonia	0:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	4	26	theme	E3	586:587	arg1	extracts					589:596	E1 and E3 extracts	579:596	E1 and E3 extracts	579:596	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	1	27	theme	waste	106:110	arg1	biomass					112:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass	87:118	Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated.
31324025	0	28	theme	Bioactive	29:37	arg1	Extracts					39:46	Bioactive Extracts	29:46	Bioactive Extracts from Posidonia	29:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	2	29	dep	E1	382:383	arg1	non-soluble					407:417	non-soluble	407:417	non-soluble	407:417	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	2	29	dep	E1	382:383	arg1	ethanol					386:392	ethanol soluble	386:400	E1: ethanol soluble; E2: non-soluble	382:417	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	2	29	dep	E1	382:383	arg1	E2					403:404	E2	403:404	E1: ethanol soluble; E2: non-soluble	382:417	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	1	30	theme	bioactive	205:213	arg1	properties					215:224	their bioactive properties	199:224	their bioactive properties	199:224	Posidonia oceanica waste biomass has been valorised to produce extracts by means of different methodologies and their bioactive properties have been evaluated.
31324025	7	31	theme	extracts	1167:1174	arg1	production					1143:1152	the production	1139:1152	the production of bioactive extracts	1139:1174	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	6	32	theme	norovirus	1062:1070	arg1	infectivity					1017:1027	the infectivity	1013:1027	the infectivity of feline calicivirus and murine norovirus	1013:1070	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	7	33	theme	bioactive	1157:1165	arg1	extracts					1167:1174	bioactive extracts	1157:1174	bioactive extracts	1157:1174	These results show the potential of P. oceanica waste biomass for the production of bioactive extracts.
31324025	6	34	theme	murine	1055:1060	arg1	norovirus					1062:1070	murine norovirus	1055:1070	murine norovirus	1055:1070	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	0	35	theme	Extracts	39:46	arg1	Characterization					9:24	In-Depth Characterization	0:24	In-Depth Characterization of Bioactive Extracts from Posidonia	0:61	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	6	36	theme	fungi	959:963	arg1	growth					931:936	the growth	927:936	the growth of several foodborne fungi	927:963	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	5	37	theme	polysaccharides	877:891	arg1	presence					852:859	the presence	848:859	the presence of proteins and polysaccharides	848:891	All the extracts showed remarkably high antioxidant capacity, which was not only related to phenolic compounds but also to the presence of proteins and polysaccharides.
31324025	2	38	theme	Water-based	247:257	arg1	extracts					259:266	Water-based extracts	247:266	Water-based extracts	247:266	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	6	39	theme	foodborne	949:957	arg1	fungi					959:963	several foodborne fungi	941:963	several foodborne fungi	941:963	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	4	40	theme	E1	579:580	arg1	extracts					589:596	E1 and E3 extracts	579:596	E1 and E3 extracts	579:596	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	3	41	with	protocol	446:453	arg1	solvents					468:475	organic solvents	460:475	organic solvents	460:475	Moreover, a conventional protocol with organic solvents was applied, yielding E3 extracts.
31324025	2	42	theme	ultrasound-assisted	288:306	arg1	methods					322:328	ultrasound-assisted and hot water methods	288:328	ultrasound-assisted and hot water methods	288:328	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
31324025	6	43	theme	calicivirus	1039:1049	arg1	infectivity					1017:1027	the infectivity	1013:1027	the infectivity of feline calicivirus and murine norovirus	1013:1070	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	3	44	theme	E3	499:500	arg1	extracts					502:509	E3 extracts	499:509	E3 extracts	499:509	Moreover, a conventional protocol with organic solvents was applied, yielding E3 extracts.
31324025	6	45	theme	feline	1032:1037	arg1	calicivirus					1039:1049	feline calicivirus	1032:1049	feline calicivirus	1032:1049	All E2 and E3 extracts inhibited the growth of several foodborne fungi, while only E3 extracts decreased substantially the infectivity of feline calicivirus and murine norovirus.
31324025	4	46	theme	E2	657:658	arg1	extracts					660:667	E2 extracts	657:667	E2 extracts	657:667	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	4	46	theme	E2	657:658	arg1	mixture					676:682	a mixture	674:682	a mixture of minerals, proteins and carbohydrates	674:722	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	4	47	theme	proteins	697:704	arg1	extracts					660:667	E2 extracts	657:667	E2 extracts	657:667	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	4	47	theme	proteins	697:704	arg1	mixture					676:682	a mixture	674:682	a mixture of minerals, proteins and carbohydrates	674:722	Compositional and structural characterization confirmed that while E1 and E3 extracts were mainly composed of minerals and lipids, respectively, E2 extracts were a mixture of minerals, proteins and carbohydrates.
31324025	0	48	theme	Waste	72:76	arg1	Biomass					78:84	Waste Biomass	72:84	Waste Biomass	72:84	In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass.
31324025	2	49	theme	soluble	394:400	arg1	ethanol					386:392	ethanol soluble	386:400	E1: ethanol soluble; E2: non-soluble	382:417	Water-based extracts were produced using ultrasound-assisted and hot water methods and classified according to their ethanol-affinity (E1: ethanol soluble; E2: non-soluble).
29425873	2	0	theme	high-throughput	476:490	arg1	technology					503:512	high-throughput sequencing technology	476:512	high-throughput sequencing technology	476:512	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	1	from	effects	420:426	arg1	microbiota					443:452	gut microbiota	439:452	gut microbiota	439:452	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	1	2	from	algae	174:178	arg1	polysaccharides					146:160	Sulfated polysaccharides	137:160	Sulfated polysaccharides from marine algae	137:178	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	2	3	dep	in	286:287	arg1	vitro					289:293	vitro	289:293	vitro	289:293	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	3	4	theme	gut	701:703	arg1	microbiota					705:714	gut microbiota	701:714	gut microbiota	701:714	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	5	theme	Salivary	515:522	arg1	amylase					524:530	Salivary amylase	515:530	Salivary amylase	515:530	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	2	6	from	fermentability	313:326	arg1	AnPs					391:394	AnPs	391:394	AnPs	391:394	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	6	from	fermentability	313:326	arg1	nodosum					382:388	the brown seaweed Ascophyllum nodosum	352:388	the brown seaweed Ascophyllum nodosum (AnPs)	352:395	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	0	7	from	Digestibility	0:12	arg1	nodosum					76:82	the brown seaweed Ascophyllum nodosum	46:82	the brown seaweed Ascophyllum nodosum	46:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	0	7	from	Digestibility	0:12	arg1	microbiota					116:125	the human gut microbiota	102:125	the human gut microbiota in vitro	102:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	2	8	theme	in	286:287	arg1	digestibility					295:307	digestibility	295:307	digestibility	295:307	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	5	9	theme	SCFA	961:964	arg1	content					966:972	the total SCFA content	951:972	the total SCFA content after fermentation	951:991	Furthermore, the total SCFA content after fermentation increased significantly.
29425873	0	10	theme	human	106:110	arg1	microbiota					116:125	the human gut microbiota	102:125	the human gut microbiota in vitro	102:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	6	11	contain	have	1050:1053	arg1	AnPs					1045:1048	AnPs	1045:1048	AnPs	1045:1048	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	6	11	contain	have	1050:1053	arg2	potential					1055:1063	potential uses	1055:1068	potential uses as functional food components to improve human gut health	1055:1126	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	4	12	theme	Bacteroidetes	841:853	arg1	abundance					828:836	the relative abundance	815:836	the relative abundance of Bacteroidetes and Firmicutes	815:868	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	3	13	theme	artificial	533:542	arg1	juice					552:556	artificial gastric juice	533:556	artificial gastric juice	533:556	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	1	14	theme	marine	167:172	arg1	algae					174:178	marine algae	167:178	marine algae	167:178	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	3	15	theme	gastric	544:550	arg1	juice					552:556	artificial gastric juice	533:556	artificial gastric juice	533:556	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	4	16	theme	obesity	929:935	arg1	risk					921:924	the risk	917:924	the risk of obesity	917:935	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	6	17	theme	food	1084:1087	arg1	components					1089:1098	functional food components	1073:1098	functional food components	1073:1098	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	4	18	theme	Firmicutes	859:868	arg1	abundance					828:836	the relative abundance	815:836	the relative abundance of Bacteroidetes and Firmicutes	815:868	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	6	19	theme	functional	1073:1082	arg1	components					1089:1098	functional food components	1073:1098	functional food components	1073:1098	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	2	20	theme	AnPs	431:434	arg1	effects					420:426	the effects	416:426	the effects of AnPs on gut microbiota	416:452	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	3	21	theme	sugar	649:653	arg1	weight					621:626	the molecular weight	607:626	the molecular weight of AnPs and reducing sugar	607:653	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	2	22	from	nodosum	382:388	arg1	digestibility					295:307	digestibility	295:307	digestibility	295:307	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	22	from	nodosum	382:388	arg1	polysaccharides					331:345	polysaccharides	331:345	polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs)	331:395	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	22	from	nodosum	382:388	arg1	fermentability					313:326	fermentability	313:326	fermentability	313:326	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	4	23	theme	gut	769:771	arg1	microbiota					773:782	the gut microbiota	765:782	the gut microbiota	765:782	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	1	24	theme	various	188:194	arg1	bioactivities					196:208	various bioactivities	188:208	various bioactivities with potential benefits for human health and well-being	188:264	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	0	25	theme	polysaccharide	26:39	arg1	Digestibility					0:12	Digestibility	0:12	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum	0:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	0	25	theme	polysaccharide	26:39	arg1	effect					92:97	its effect	88:97	its effect on the human gut microbiota in vitro	88:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	5	26	theme	total	955:959	arg1	content					966:972	the total SCFA content	951:972	the total SCFA content after fermentation	951:991	Furthermore, the total SCFA content after fermentation increased significantly.
29425873	0	27	theme	sulfated	17:24	arg1	polysaccharide					26:39	sulfated polysaccharide	17:39	sulfated polysaccharide from the brown seaweed Ascophyllum nodosum	17:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	3	28	contain	had	580:582	arg1	juice					552:556	artificial gastric juice	533:556	artificial gastric juice	533:556	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	28	contain	had	580:582	arg1	juice					574:578	intestinal juice	563:578	intestinal juice	563:578	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	28	contain	had	580:582	arg1	amylase					524:530	Salivary amylase	515:530	Salivary amylase	515:530	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	28	contain	had	580:582	arg2	effect					587:592	no effect	584:592	no effect	584:592	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	0	29	theme	gut	112:114	arg1	microbiota					116:125	the human gut microbiota	102:125	the human gut microbiota in vitro	102:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	3	30	theme	AnPs	631:634	arg1	weight					621:626	the molecular weight	607:626	the molecular weight of AnPs and reducing sugar	607:653	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	31	theme	intestinal	563:572	arg1	juice					574:578	intestinal juice	563:578	intestinal juice	563:578	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	1	32	with	bioactivities	196:208	arg1	benefits					225:232	potential benefits	215:232	potential benefits for human health and well-being	215:264	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	1	33	theme	potential	215:223	arg1	benefits					225:232	potential benefits	215:232	potential benefits for human health and well-being	215:264	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	0	34	from	nodosum	76:82	arg1	Digestibility					0:12	Digestibility	0:12	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum	0:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	0	34	from	nodosum	76:82	arg1	polysaccharide					26:39	sulfated polysaccharide	17:39	sulfated polysaccharide from the brown seaweed Ascophyllum nodosum	17:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	0	34	from	nodosum	76:82	arg1	effect					92:97	its effect	88:97	its effect on the human gut microbiota in vitro	88:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	2	35	theme	gut	439:441	arg1	microbiota					443:452	gut microbiota	439:452	gut microbiota	439:452	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	4	36	theme	relative	819:826	arg1	abundance					828:836	the relative abundance	815:836	the relative abundance of Bacteroidetes and Firmicutes	815:868	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	2	37	theme	polysaccharides	331:345	arg1	digestibility					295:307	digestibility	295:307	digestibility	295:307	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	37	theme	polysaccharides	331:345	arg1	fermentability					313:326	fermentability	313:326	fermentability	313:326	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	4	38	theme	microbiota	773:782	arg1	composition					750:760	the composition	746:760	the composition of the gut microbiota	746:782	AnPs significantly modulated the composition of the gut microbiota; in particular, they increased the relative abundance of Bacteroidetes and Firmicutes, suggesting the potential for AnPs to decrease the risk of obesity.
29425873	3	39	theme	reducing	640:647	arg1	sugar					649:653	reducing sugar	640:653	reducing sugar	640:653	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	3	40	theme	molecular	611:619	arg1	weight					621:626	the molecular weight	607:626	the molecular weight of AnPs and reducing sugar	607:653	Salivary amylase, artificial gastric juice, and intestinal juice had no effect on AnPs, but the molecular weight of AnPs and reducing sugar decreased significantly after fermentation by gut microbiota.
29425873	6	41	theme	human	1111:1115	arg1	health					1121:1126	human gut health	1111:1126	human gut health	1111:1126	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	1	42	theme	human	238:242	arg1	health					244:249	human health	238:249	human health	238:249	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	0	43	from	effect	92:97	arg1	nodosum					76:82	the brown seaweed Ascophyllum nodosum	46:82	the brown seaweed Ascophyllum nodosum	46:82	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	0	43	from	effect	92:97	arg1	microbiota					116:125	the human gut microbiota	102:125	the human gut microbiota in vitro	102:134	Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro.
29425873	6	44	dep	potential	1055:1063	arg1	uses					1065:1068	uses	1065:1068	uses	1065:1068	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	6	45	theme	gut	1117:1119	arg1	health					1121:1126	human gut health	1111:1126	human gut health	1111:1126	These results suggest that AnPs have potential uses as functional food components to improve human gut health.
29425873	2	46	theme	sequencing	492:501	arg1	technology					503:512	high-throughput sequencing technology	476:512	high-throughput sequencing technology	476:512	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	1	47	theme	Sulfated	137:144	arg1	polysaccharides					146:160	Sulfated polysaccharides	137:160	Sulfated polysaccharides from marine algae	137:178	Sulfated polysaccharides from marine algae exhibit various bioactivities with potential benefits for human health and well-being.
29425873	2	48	from	digestibility	295:307	arg1	AnPs					391:394	AnPs	391:394	AnPs	391:394	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
29425873	2	48	from	digestibility	295:307	arg1	nodosum					382:388	the brown seaweed Ascophyllum nodosum	352:388	the brown seaweed Ascophyllum nodosum (AnPs)	352:395	In this study, the in vitro digestibility and fermentability of polysaccharides from the brown seaweed Ascophyllum nodosum (AnPs) were examined, and the effects of AnPs on gut microbiota were determined using high-throughput sequencing technology.
30096842	6	0	contain	has	1515:1517	arg2	type					1426:1429	this type	1421:1429	this type of layered scaffolds	1421:1450	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	0	contain	has	1515:1517	arg2	analogue					1471:1478	an analogue	1468:1478	an analogue of the osteochondral matrix	1468:1506	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	0	contain	has	1515:1517	arg2	potential					1519:1527	potential	1519:1527	potential	1519:1527	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	0	contain	has	1515:1517	arg1	it					1512:1513	it	1512:1513	it	1512:1513	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	4	1	with	molecules	903:911	arg1	weight					936:941	limited molecular weight	918:941	limited molecular weight	918:941	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	5	2	theme	chondrocytes	1160:1171	arg1	growth					1145:1150	the growth	1141:1150	the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively	1141:1257	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	3	3	theme	nanofibrous	659:669	arg1	unit					680:683	the integrated nanofibrous membrane unit	644:683	the integrated nanofibrous membrane unit	644:683	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	5	4	theme	bony	1388:1391	arg1	layer					1393:1397	the bony layer	1384:1397	the bony layer	1384:1397	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	1	5	theme	silk	163:166	arg1	SF					177:178	SF	177:178	SF	177:178	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	5	theme	silk	163:166	arg1	fibroin					168:174	silk fibroin	163:174	silk fibroin (SF)	163:179	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	6	theme	gradient	330:337	arg1	compositional					339:351	gradient compositional	330:351	gradient compositional	330:351	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	4	7	theme	membrane	855:862	arg1	permeable					885:893	permeable	885:893	permeable	885:893	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	4	7	theme	membrane	855:862	arg1	unit					864:867	The nanofibrous membrane unit	839:867	The nanofibrous membrane unit	839:867	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	4	7	theme	membrane	855:862	arg1	able					951:954	able	951:954	able	951:954	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	1	8	with	units	319:323	arg1	compositional					339:351	gradient compositional	330:351	gradient compositional	330:351	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	8	with	units	319:323	arg1	features					368:375	structural features	357:375	structural features	357:375	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	9	theme	CH/SF/HA	227:234	arg1	composites					236:245	CH/SF/HA composites	227:245	CH/SF/HA composites	227:245	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	0	10	theme	Calcified	107:115	arg1	Layer					117:121	Calcified Layer	107:121	Calcified Layer	107:121	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	3	11	theme	middle	703:708	arg1	layer					710:714	the middle layer	699:714	the middle layer for mimicking the calcified layer and the top layer	699:766	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	4	12	theme	nanofibrous	843:853	arg1	permeable					885:893	permeable	885:893	permeable	885:893	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	4	12	theme	nanofibrous	843:853	arg1	unit					864:867	The nanofibrous membrane unit	839:867	The nanofibrous membrane unit	839:867	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	4	12	theme	nanofibrous	843:853	arg1	able					951:954	able	951:954	able	951:954	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	6	13	theme	matrix	1501:1506	arg1	type					1426:1429	this type	1421:1429	this type of layered scaffolds	1421:1450	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	13	theme	matrix	1501:1506	arg1	potential					1519:1527	potential	1519:1527	potential	1519:1527	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	13	theme	matrix	1501:1506	arg1	analogue					1471:1478	an analogue	1468:1478	an analogue of the osteochondral matrix	1468:1506	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	0	14	theme	Osteochondral	126:138	arg1	Matrix					140:145	Osteochondral Matrix	126:145	Osteochondral Matrix	126:145	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	2	15	dep	CH/HA	427:431	arg1	composites					443:452	composites	443:452	composites	443:452	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	6	16	theme	osteochondral	1487:1499	arg1	matrix					1501:1506	the osteochondral matrix	1483:1506	the osteochondral matrix	1483:1506	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	2	17	theme	membrane	390:397	arg1	unit					399:402	The optimal membrane unit	378:402	The optimal membrane unit	378:402	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	4	18	theme	limited	918:924	arg1	weight					936:941	limited molecular weight	918:941	limited molecular weight	918:941	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	1	19	theme	structural	357:366	arg1	features					368:375	structural features	357:375	structural features	357:375	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	2	20	theme	optimal	382:388	arg1	unit					399:402	The optimal membrane unit	378:402	The optimal membrane unit	378:402	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	0	21	theme	Nanofibrous	15:25	arg1	Unit					36:39	Chitosan-Based Nanofibrous Membrane Unit	0:39	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.	0:146	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	5	22	theme	neotissue	1371:1379	arg1	preservation					1309:1320	the phenotype preservation	1295:1320	the phenotype preservation of seeded chondrocytes	1295:1343	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	5	22	theme	neotissue	1371:1379	arg1	mineralization					1353:1366	the mineralization	1349:1366	the mineralization of neotissue	1349:1379	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	0	23	theme	Chitosan-Based	0:13	arg1	Unit					36:39	Chitosan-Based Nanofibrous Membrane Unit	0:39	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.	0:146	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	4	24	theme	cross	999:1003	arg1	unit					1009:1012	cross the unit	999:1012	cross the unit	999:1012	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	3	25	theme	calcified	734:742	arg1	layer					744:748	the calcified layer	730:748	the calcified layer	730:748	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	3	26	theme	bottom	545:550	arg1	layer					552:556	The bottom layer	541:556	The bottom layer of the scaffold	541:572	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	3	27	theme	CH/SF	790:794	arg1	composites					796:805	CH/SF composites	790:805	CH/SF composites for acting as a chondral layer	790:836	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	0	28	theme	Membrane	27:34	arg1	Unit					36:39	Chitosan-Based Nanofibrous Membrane Unit	0:39	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.	0:146	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	1	29	theme	resulting	255:263	arg1	composites					265:274	the resulting composites	251:274	the resulting composites	251:274	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	6	30	theme	osteochondral	1532:1544	arg1	repair					1546:1551	osteochondral repair	1532:1551	osteochondral repair	1532:1551	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	4	31	theme	osteochondral	1073:1085	arg1	matrix					1087:1092	the osteochondral matrix	1069:1092	the osteochondral matrix	1069:1092	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	6	32	theme	layered	1434:1440	arg1	scaffolds					1442:1450	layered scaffolds	1434:1450	layered scaffolds	1434:1450	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	0	33	theme	Gradient	46:53	arg1	Compositional					55:67	Gradient Compositional	46:67	Gradient Compositional	46:67	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	3	34	theme	subchondral	625:635	arg1	layer					637:641	a subchondral layer	623:641	a subchondral layer	623:641	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	3	34	theme	subchondral	625:635	arg1	it					610:611	it	610:611	it	610:611	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	2	35	theme	osteochondral	519:531	arg1	repair					533:538	osteochondral repair	519:538	osteochondral repair	519:538	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	0	36	with	Unit	36:39	arg1	Compositional					55:67	Gradient Compositional	46:67	Gradient Compositional	46:67	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	0	36	with	Unit	36:39	arg1	Features					84:91	Structural Features	73:91	Structural Features	73:91	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	4	37	from	layer	1060:1064	arg1	matrix					1087:1092	the osteochondral matrix	1069:1092	the osteochondral matrix	1069:1092	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	4	38	theme	seeded	971:976	arg1	cells					978:982	the seeded cells	967:982	the seeded cells	967:982	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	3	39	theme	top	758:760	arg1	layer					762:766	the top layer	754:766	the top layer	754:766	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	0	40	theme	Structural	73:82	arg1	Features					84:91	Structural Features	73:91	Structural Features	73:91	Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix.
30096842	3	41	theme	scaffold	565:572	arg1	layer					552:556	The bottom layer	541:556	The bottom layer of the scaffold	541:572	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	5	42	theme	Layered	1095:1101	arg1	scaffolds					1103:1111	Layered scaffolds	1095:1111	Layered scaffolds	1095:1111	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	3	43	theme	membrane	671:678	arg1	unit					680:683	the integrated nanofibrous membrane unit	644:683	the integrated nanofibrous membrane unit	644:683	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	1	44	used	used	211:214	arg2	hydroxyapatite					186:199	hydroxyapatite	186:199	hydroxyapatite (HA)	186:204	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	44	used	used	211:214	arg2	Chitosan					148:155	Chitosan	148:155	Chitosan (CH)	148:160	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	44	used	used	211:214	arg2	fibroin					168:174	silk fibroin	163:174	silk fibroin (SF)	163:179	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	44	used	used	211:214	arg2	CH					158:159	CH	158:159	CH	158:159	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	44	used	used	211:214	arg2	SF					177:178	SF	177:178	SF	177:178	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	1	44	used	used	211:214	arg2	HA					202:203	HA	202:203	HA	202:203	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	2	45	used	used	408:411	arg2	unit					399:402	The optimal membrane unit	378:402	The optimal membrane unit	378:402	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	3	46	theme	chondral	823:830	arg1	layer					832:836	a chondral layer	821:836	a chondral layer	821:836	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	1	47	theme	nanofibrous	298:308	arg1	units					319:323	nanofibrous membrane units	298:323	nanofibrous membrane units with gradient compositional and structural features	298:375	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	3	48	theme	integrated	648:657	arg1	unit					680:683	the integrated nanofibrous membrane unit	644:683	the integrated nanofibrous membrane unit	644:683	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	5	49	theme	osteoblasts	1177:1187	arg1	growth					1145:1150	the growth	1141:1150	the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively	1141:1257	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	5	50	theme	seeded	1325:1330	arg1	chondrocytes					1332:1343	seeded chondrocytes	1325:1343	seeded chondrocytes	1325:1343	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	6	51	theme	scaffolds	1442:1450	arg1	analogue					1471:1478	an analogue	1468:1478	an analogue of the osteochondral matrix	1468:1506	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	51	theme	scaffolds	1442:1450	arg1	potential					1519:1527	potential	1519:1527	potential	1519:1527	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	6	51	theme	scaffolds	1442:1450	arg1	type					1426:1429	this type	1421:1429	this type of layered scaffolds	1421:1450	Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.
30096842	5	52	theme	chondrocytes	1332:1343	arg1	preservation					1309:1320	the phenotype preservation	1295:1320	the phenotype preservation of seeded chondrocytes	1295:1343	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	5	52	theme	chondrocytes	1332:1343	arg1	mineralization					1353:1366	the mineralization	1349:1366	the mineralization of neotissue	1349:1379	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	5	53	theme	phenotype	1299:1307	arg1	preservation					1309:1320	the phenotype preservation	1295:1320	the phenotype preservation of seeded chondrocytes	1295:1343	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	4	54	theme	calcified	1050:1058	arg1	layer					1060:1064	the calcified layer	1046:1064	the calcified layer in the osteochondral matrix	1046:1092	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
30096842	2	55	theme	scaffold	489:496	arg1	type					469:472	a type	467:472	a type of three-layer scaffold that is intended for osteochondral repair	467:538	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	5	56	theme	bony	1234:1237	arg1	layer					1239:1243	bony layer	1234:1243	bony layer	1234:1243	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	3	57	theme	CH/HA	589:593	arg1	composites					595:604	CH/HA composites	589:604	CH/HA composites	589:604	The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer.
30096842	2	58	theme	three-layer	477:487	arg1	scaffold					489:496	three-layer scaffold	477:496	three-layer scaffold that is intended for osteochondral repair	477:538	The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair.
30096842	1	59	theme	membrane	310:317	arg1	units					319:323	nanofibrous membrane units	298:323	nanofibrous membrane units with gradient compositional and structural features	298:375	Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features.
30096842	5	60	theme	chondral	1215:1222	arg1	layer					1224:1228	their chondral layer	1209:1228	their chondral layer	1209:1228	Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer.
30096842	4	61	theme	molecular	926:934	arg1	weight					936:941	limited molecular weight	918:941	limited molecular weight	918:941	The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix.
31518624	2	0	from	glucose	281:287	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	4	1	theme	scavenging	521:530	arg1	effects					532:538	strong scavenging effects	514:538	strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical	514:608	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	4	2	theme	superoxide	571:580	arg1	anions					582:587	superoxide anions	571:587	superoxide anions	571:587	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	5	3	theme	GCP-WS	736:741	arg1	abilities					723:731	the corresponding scavenging abilities	694:731	the corresponding scavenging abilities of GCP-WS	694:741	Results displayed that at the concentration of 5 mg/mL, the corresponding scavenging abilities of GCP-WS reached 83.08 ± 2.58%, 85.69 ± 1.62%, 79.38 ± 2.03% and 81.39 ± 1.47%, respectively.
31518624	4	4	theme	strong	514:519	arg1	effects					532:538	strong scavenging effects	514:538	strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical	514:608	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	2	5	from	fructose	298:305	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	2	6	from	xylose	290:295	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	4	7	theme	hydroxyl	593:600	arg1	radical					602:608	hydroxyl radical	593:608	hydroxyl radical	593:608	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	4	8	theme	Antioxidant	475:485	arg1	assays					487:492	Antioxidant assays	475:492	Antioxidant assays	475:492	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	2	9	from	acid	322:325	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	2	10	theme	21.46:1.58:1.11:55.15:36.37:7.04:7.34	347:383	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	4	11	from	effects	532:538	arg1	radical					548:554	DPPH radical	543:554	DPPH radical	543:554	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	4	11	from	effects	532:538	arg1	radical					562:568	ABTS radical	557:568	ABTS radical	557:568	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	4	11	from	effects	532:538	arg1	anions					582:587	superoxide anions	571:587	superoxide anions	571:587	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	4	11	from	effects	532:538	arg1	radical					602:608	hydroxyl radical	593:608	hydroxyl radical	593:608	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	6	12	theme	antioxidant	887:897	arg1	agent					899:903	an effective antioxidant agent	874:903	an effective antioxidant agent	874:903	GCP-WS shows the potential for development as an effective antioxidant agent.
31518624	1	13	theme	Chaetomium	157:166	arg1	6882					183:186	Chaetomium globosum CGMCC 6882	157:186	Chaetomium globosum CGMCC 6882 from wheat straw	157:203	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	6	14	theme	effective	877:885	arg1	agent					899:903	an effective antioxidant agent	874:903	an effective antioxidant agent	874:903	GCP-WS shows the potential for development as an effective antioxidant agent.
31518624	4	15	dep	revealed	494:501	arg1	had					510:512	had	510:512	revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner	494:635	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	3	16	theme	GCP-WS	417:422	arg1	weight					407:412	molecular weight	397:412	molecular weight of GCP-WS	397:422	Meanwhile, molecular weight of GCP-WS was 3.538 × 104 Da with a polydispersity of 1.043.
31518624	0	17	theme	Antioxidant	0:10	arg1	activity					12:19	Antioxidant activity	0:19	Antioxidant activity of a polysaccharide	0:39	Antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882.
31518624	4	18	theme	ABTS	557:560	arg1	radical					562:568	ABTS radical	557:568	ABTS radical	557:568	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	1	19	theme	present	88:94	arg1	work					96:99	present work	88:99	present work	88:99	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	1	20	theme	CGMCC	177:181	arg1	6882					183:186	Chaetomium globosum CGMCC 6882	157:186	Chaetomium globosum CGMCC 6882 from wheat straw	157:203	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	5	21	theme	corresponding	698:710	arg1	abilities					723:731	the corresponding scavenging abilities	694:731	the corresponding scavenging abilities of GCP-WS	694:741	Results displayed that at the concentration of 5 mg/mL, the corresponding scavenging abilities of GCP-WS reached 83.08 ± 2.58%, 85.69 ± 1.62%, 79.38 ± 2.03% and 81.39 ± 1.47%, respectively.
31518624	5	22	theme	scavenging	712:721	arg1	abilities					723:731	the corresponding scavenging abilities	694:731	the corresponding scavenging abilities of GCP-WS	694:741	Results displayed that at the concentration of 5 mg/mL, the corresponding scavenging abilities of GCP-WS reached 83.08 ± 2.58%, 85.69 ± 1.62%, 79.38 ± 2.03% and 81.39 ± 1.47%, respectively.
31518624	3	23	theme	1.043	468:472	arg1	polydispersity					450:463	a polydispersity	448:463	a polydispersity of 1.043	448:472	Meanwhile, molecular weight of GCP-WS was 3.538 × 104 Da with a polydispersity of 1.043.
31518624	1	24	theme	wheat	193:197	arg1	straw					199:203	wheat straw	193:203	wheat straw	193:203	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	0	25	theme	polysaccharide	26:39	arg1	activity					12:19	Antioxidant activity	0:19	Antioxidant activity of a polysaccharide	0:39	Antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882.
31518624	0	26	theme	Chaetomium	53:62	arg1	CGMCC					73:77	Chaetomium globosum CGMCC 6882	53:82	Chaetomium globosum CGMCC 6882	53:82	Antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882.
31518624	2	27	theme	Monosaccharide	206:219	arg1	composition					221:231	Monosaccharide composition	206:231	Monosaccharide composition of GCP-WS	206:241	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	1	28	from	straw	199:203	arg1	6882					183:186	Chaetomium globosum CGMCC 6882	157:186	Chaetomium globosum CGMCC 6882 from wheat straw	157:203	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	3	29	theme	molecular	397:405	arg1	weight					407:412	molecular weight	397:412	molecular weight of GCP-WS	397:422	Meanwhile, molecular weight of GCP-WS was 3.538 × 104 Da with a polydispersity of 1.043.
31518624	5	30	theme	5 mg/mL	685:691	arg1	concentration					668:680	the concentration	664:680	the concentration of 5 mg/mL	664:691	Results displayed that at the concentration of 5 mg/mL, the corresponding scavenging abilities of GCP-WS reached 83.08 ± 2.58%, 85.69 ± 1.62%, 79.38 ± 2.03% and 81.39 ± 1.47%, respectively.
31518624	1	31	dep	Chaetomium	157:166	arg1	globosum					168:175	globosum	168:175	globosum	168:175	In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw.
31518624	2	32	theme	glucuronic	311:320	arg1	acid					322:325	glucuronic acid	311:325	glucuronic acid	311:325	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	2	32	theme	glucuronic	311:320	arg1	rhamnose					247:254	rhamnose	247:254	rhamnose	247:254	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	4	33	theme	DPPH	543:546	arg1	radical					548:554	DPPH radical	543:554	DPPH radical	543:554	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	3	34	with	3.538 × 104 Da	428:441	arg1	polydispersity					450:463	a polydispersity	448:463	a polydispersity of 1.043	448:472	Meanwhile, molecular weight of GCP-WS was 3.538 × 104 Da with a polydispersity of 1.043.
31518624	2	35	from	galactose	270:278	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	4	36	theme	dose-dependent	615:628	arg1	manner					630:635	a dose-dependent manner	613:635	a dose-dependent manner	613:635	Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, superoxide anions and hydroxyl radical in a dose-dependent manner.
31518624	0	37	theme	globosum	64:71	arg1	CGMCC					73:77	Chaetomium globosum CGMCC 6882	53:82	Chaetomium globosum CGMCC 6882	53:82	Antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882.
31518624	2	38	theme	GCP-WS	236:241	arg1	composition					221:231	Monosaccharide composition	206:231	Monosaccharide composition of GCP-WS	206:241	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31518624	2	39	theme	molar	332:336	arg1	ratio					338:342	a molar ratio	330:342	a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34	330:383	Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34.
31258789	12	0	theme	glycome	2038:2044	arg1	profiles					2046:2053	IgG glycome profiles	2034:2053	IgG glycome profiles in a large number of preoperative peritoneal metastasis serums	2034:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	4	1	theme	G	606:606	arg1	N-glycome					614:622	Immunoglobulin G (IgG) N-glycome	591:622	Immunoglobulin G (IgG) N-glycome	591:622	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	14	2	theme	metastasis	2239:2248	arg1	understanding					2211:2223	the understanding	2207:2223	the understanding of peritoneal metastasis	2207:2248	These findings enhance the understanding of peritoneal metastasis.
31258789	7	3	theme	peritoneal	1156:1165	arg1	group					1178:1182	peritoneal metastasis group	1156:1182	peritoneal metastasis group	1156:1182	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	1	4	theme	advanced	253:260	arg1	cancer					270:275	advanced gastric cancer	253:275	advanced gastric cancer	253:275	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	9	5	theme	0.77	1665:1668	arg1	value					1629:1633	characteristic curve (AUC) value	1602:1633	characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer	1602:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	11	6	with	patients	1889:1896	arg1	cancer					1911:1916	gastric cancer	1903:1916	gastric cancer	1903:1916	Two patients with gastric cancer were selected to perform and demonstrate the usage of the diagnostic workflow.
31258789	9	7	theme	validation	1671:1680	arg1	0.77					1665:1668	0.77	1665:1668	0.77	1665:1668	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	7	theme	validation	1671:1680	arg1	cohort					1682:1687	validation cohort	1671:1687	validation cohort	1671:1687	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	5	8	theme	training	891:898	arg1	n=249					908:912	n=249	908:912	n=249	908:912	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	5	8	theme	training	891:898	arg1	cohort					900:905	training cohort	891:905	training cohort (n=249)	891:913	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	10	9	theme	diagnostic	1793:1802	arg1	performance					1804:1814	The diagnostic performance	1789:1814	The diagnostic performance of this model	1789:1828	The diagnostic performance of this model was further validated in a combined cohort (AUC=0.79).
31258789	8	10	theme	IgG	1242:1244	arg1	glycosylation					1246:1258	IgG glycosylation	1242:1258	IgG glycosylation	1242:1258	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	12	11	theme	serums	2111:2116	arg1	number					2066:2071	a large number	2058:2071	a large number of preoperative peritoneal metastasis serums	2058:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	0	12	theme	Gastric	118:124	arg1	Cancer					126:131	Advanced Gastric Cancer	109:131	Advanced Gastric Cancer	109:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	8	13	gly	sialylation	1272:1282	arg1	metastasis					1330:1339	peritoneal metastasis	1319:1339	peritoneal metastasis	1319:1339	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	2	14	theme	operative	302:310	arg1	diagnosis					312:320	operative diagnosis	302:320	operative diagnosis	302:320	However, operative diagnosis could bring both mental and physical trauma and economic burden for patients.
31258789	1	15	theme	gastric	203:209	arg1	cancer					211:216	gastric cancer	203:216	gastric cancer	203:216	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	9	16	theme	good	1704:1707	arg1	potential					1709:1717	good potential	1704:1717	good potential	1704:1717	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	4	17	theme	potential	659:667	arg1	biomarkers					669:678	potential biomarkers	659:678	potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set	659:801	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	15	18	theme	reliable	2332:2339	arg1	predictor					2341:2349	a reliable predictor	2330:2349	a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer	2330:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	15	18	theme	reliable	2332:2339	arg1	glyco-model					2309:2319	the newly established glyco-model	2287:2319	the newly established glyco-model	2287:2319	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	8	19	gly	fucosylation	1288:1299	arg1	metastasis					1330:1339	peritoneal metastasis	1319:1339	peritoneal metastasis	1319:1339	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	5	20	theme	patients	843:850	arg1	total					815:819	A total	813:819	A total of 373 gastric cancer patients	813:850	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	6	21	theme	IgG	950:952	arg1	composition					964:974	The IgG N-glycome composition	946:974	The IgG N-glycome composition	946:974	The IgG N-glycome composition was analyzed by ultra-performance liquid chromatography.
31258789	15	22	from	predictor	2341:2349	arg1	patients					2395:2402	patients	2395:2402	patients with advanced gastric cancer	2395:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	4	23	theme	gastric	750:756	arg1	cancer					758:763	advanced gastric cancer	741:763	advanced gastric cancer	741:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	15	24	theme	metastasis	2381:2390	arg1	presence					2358:2365	the presence	2354:2365	the presence of peritoneal metastasis in patients with advanced gastric cancer	2354:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	9	25	theme	peritoneal	1737:1746	arg1	metastasis					1748:1757	peritoneal metastasis	1737:1757	peritoneal metastasis	1737:1757	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	12	26	theme	peritoneal	2089:2098	arg1	serums					2111:2116	preoperative peritoneal metastasis serums	2076:2116	preoperative peritoneal metastasis serums	2076:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	4	27	theme	peritoneal	714:723	arg1	metastasis					725:734	peritoneal metastasis	714:734	peritoneal metastasis	714:734	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	13	28	theme	peritoneal	2161:2170	arg1	metastasis					2172:2181	peritoneal metastasis	2161:2181	peritoneal metastasis	2161:2181	The IgG glycan was highly associated with peritoneal metastasis.
31258789	11	29	theme	diagnostic	1976:1985	arg1	workflow					1987:1994	the diagnostic workflow	1972:1994	the diagnostic workflow	1972:1994	Two patients with gastric cancer were selected to perform and demonstrate the usage of the diagnostic workflow.
31258789	1	30	theme	Peritoneal	146:155	arg1	metastasis					157:166	Peritoneal metastasis	146:166	Peritoneal metastasis	146:166	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	9	31	theme	gastric	1773:1779	arg1	cancer					1781:1786	advanced gastric cancer	1764:1786	advanced gastric cancer	1764:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	8	32	theme	bisecting	1394:1402	arg1	GlcNAc					1404:1409	bisecting GlcNAc	1394:1409	bisecting GlcNAc	1394:1409	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	5	33	theme	validation	919:928	arg1	n=124					938:942	n=124	938:942	n=124	938:942	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	5	33	theme	validation	919:928	arg1	cohort					930:935	validation cohort	919:935	validation cohort (n=124)	919:943	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	15	34	from	patients	2395:2402	arg1	predictor					2341:2349	a reliable predictor	2330:2349	a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer	2330:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	15	34	from	patients	2395:2402	arg1	glyco-model					2309:2319	the newly established glyco-model	2287:2319	the newly established glyco-model	2287:2319	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	12	35	theme	preoperative	2076:2087	arg1	serums					2111:2116	preoperative peritoneal metastasis serums	2076:2116	preoperative peritoneal metastasis serums	2076:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	0	36	theme	Peritoneal	82:91	arg1	Metastasis					93:102	Peritoneal Metastasis	82:102	Peritoneal Metastasis	82:102	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	6	37	theme	ultra-performance	992:1008	arg1	chromatography					1017:1030	ultra-performance liquid chromatography	992:1030	ultra-performance liquid chromatography	992:1030	The IgG N-glycome composition was analyzed by ultra-performance liquid chromatography.
31258789	5	38	theme	cancer	836:841	arg1	patients					843:850	373 gastric cancer patients	824:850	373 gastric cancer patients	824:850	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	15	39	theme	established	2297:2307	arg1	predictor					2341:2349	a reliable predictor	2330:2349	a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer	2330:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	15	39	theme	established	2297:2307	arg1	glyco-model					2309:2319	the newly established glyco-model	2287:2319	the newly established glyco-model	2287:2319	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	9	40	theme	characteristic	1602:1615	arg1	AUC					1624:1626	AUC	1624:1626	AUC	1624:1626	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	40	theme	characteristic	1602:1615	arg1	curve					1617:1621	characteristic curve	1602:1621	characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer	1602:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	4	41	theme	sample	792:797	arg1	set					799:801	the comprehensive sample set	774:801	the comprehensive sample set	774:801	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	7	42	theme	cancer	1205:1210	arg1	group					1212:1216	advanced gastric cancer group	1188:1216	advanced gastric cancer group	1188:1216	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	3	43	theme	non-invasive	416:427	arg1	necessary					442:450	necessary	442:450	necessary	442:450	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	3	43	theme	non-invasive	416:427	arg1	biomarker					429:437	a non-invasive biomarker	414:437	a non-invasive biomarker	414:437	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	7	44	theme	advanced	1188:1195	arg1	group					1212:1216	advanced gastric cancer group	1188:1216	advanced gastric cancer group	1188:1216	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	0	45	theme	Immunoglobulin	19:32	arg1	Glycome					36:42	Serum Immunoglobulin G Glycome	13:42	Serum Immunoglobulin G Glycome	13:42	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	10	46	theme	combined	1857:1864	arg1	AUC=0.79					1874:1881	AUC=0.79	1874:1881	AUC=0.79	1874:1881	The diagnostic performance of this model was further validated in a combined cohort (AUC=0.79).
31258789	10	46	theme	combined	1857:1864	arg1	cohort					1866:1871	a combined cohort	1855:1871	a combined cohort (AUC=0.79)	1855:1882	The diagnostic performance of this model was further validated in a combined cohort (AUC=0.79).
31258789	2	47	theme	physical	350:357	arg1	trauma					359:364	both mental and physical trauma	334:364	both mental and physical trauma	334:364	However, operative diagnosis could bring both mental and physical trauma and economic burden for patients.
31258789	0	48	theme	Glycome	36:42	arg1	Value					4:8	The Value	0:8	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.	0:132	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	15	49	attach	presence	2358:2365	arg1	patients					2395:2402	patients	2395:2402	patients with advanced gastric cancer	2395:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	15	49	attach	presence	2358:2365	arg2	metastasis					2381:2390	peritoneal metastasis	2370:2390	peritoneal metastasis	2370:2390	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	2	50	theme	mental	339:344	arg1	trauma					359:364	both mental and physical trauma	334:364	both mental and physical trauma	334:364	However, operative diagnosis could bring both mental and physical trauma and economic burden for patients.
31258789	4	51	from	cancer	758:763	arg1	biomarkers					669:678	potential biomarkers	659:678	potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set	659:801	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	4	51	from	cancer	758:763	arg1	discrimination					696:709	preoperative discrimination	683:709	preoperative discrimination of peritoneal metastasis from advanced gastric cancer	683:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	9	52	theme	glycomics	1452:1460	arg1	profile					1462:1468	the significant glycomics profile	1436:1468	the significant glycomics profile	1436:1468	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	3	53	theme	patients	528:535	arg1	quality					517:523	survival quality	508:523	survival quality of patients	508:535	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	15	54	theme	advanced	2409:2416	arg1	cancer					2426:2431	advanced gastric cancer	2409:2431	advanced gastric cancer	2409:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	12	55	theme	IgG	2034:2036	arg1	profiles					2046:2053	IgG glycome profiles	2034:2053	IgG glycome profiles in a large number of preoperative peritoneal metastasis serums	2034:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	8	56	from	differences	1227:1237	arg1	glycosylation					1246:1258	IgG glycosylation	1242:1258	IgG glycosylation	1242:1258	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	4	57	theme	Immunoglobulin	591:604	arg1	IgG					609:611	IgG	609:611	IgG	609:611	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	4	57	theme	Immunoglobulin	591:604	arg1	G					606:606	Immunoglobulin G	591:606	Immunoglobulin G (IgG) N-glycome	591:622	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	14	58	theme	peritoneal	2228:2237	arg1	metastasis					2239:2248	peritoneal metastasis	2228:2248	peritoneal metastasis	2228:2248	These findings enhance the understanding of peritoneal metastasis.
31258789	1	59	theme	gastric	262:268	arg1	cancer					270:275	advanced gastric cancer	253:275	advanced gastric cancer	253:275	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	12	60	theme	large	2060:2064	arg1	number					2066:2071	a large number	2058:2071	a large number of preoperative peritoneal metastasis serums	2058:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	7	61	theme	same	1117:1120	arg1	structures					1122:1131	the same structures	1113:1131	the same structures	1113:1131	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	9	62	theme	glycan	1502:1507	arg1	GP23					1541:1544	GP23	1541:1544	GP23	1541:1544	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	62	theme	glycan	1502:1507	arg1	peaks					1509:1513	five glycan peaks	1497:1513	five glycan peaks (GP6, GP9, GP11, GP21 and GP23)	1497:1545	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	62	theme	glycan	1502:1507	arg1	GP21					1532:1535	GP21	1532:1535	GP21	1532:1535	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	4	63	from	biomarkers	669:678	arg1	cancer					758:763	advanced gastric cancer	741:763	advanced gastric cancer	741:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	4	63	from	biomarkers	669:678	arg1	discrimination					696:709	preoperative discrimination	683:709	preoperative discrimination of peritoneal metastasis from advanced gastric cancer	683:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	1	64	theme	poor	185:188	arg1	prognosis					190:198	poor prognosis	185:198	poor prognosis in gastric cancer	185:216	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	8	65	theme	Several	1219:1225	arg1	differences					1227:1237	Several differences	1219:1237	Several differences in IgG glycosylation	1219:1258	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	4	66	theme	preoperative	683:694	arg1	discrimination					696:709	preoperative discrimination	683:709	preoperative discrimination of peritoneal metastasis from advanced gastric cancer	683:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	12	67	theme	metastasis	2100:2109	arg1	serums					2111:2116	preoperative peritoneal metastasis serums	2076:2116	preoperative peritoneal metastasis serums	2076:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	15	68	theme	gastric	2418:2424	arg1	cancer					2426:2431	advanced gastric cancer	2409:2431	advanced gastric cancer	2409:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	6	69	theme	N-glycome	954:962	arg1	composition					964:974	The IgG N-glycome composition	946:974	The IgG N-glycome composition	946:974	The IgG N-glycome composition was analyzed by ultra-performance liquid chromatography.
31258789	8	70	theme	peritoneal	1319:1328	arg1	metastasis					1330:1339	peritoneal metastasis	1319:1339	peritoneal metastasis	1319:1339	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	7	71	theme	glycan	1054:1059	arg1	peaks					1061:1065	Twenty-four glycan peaks	1042:1065	Twenty-four glycan peaks	1042:1065	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	4	72	theme	advanced	741:748	arg1	cancer					758:763	advanced gastric cancer	741:763	advanced gastric cancer	741:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	7	73	theme	gastric	1197:1203	arg1	group					1212:1216	advanced gastric cancer group	1188:1216	advanced gastric cancer group	1188:1216	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	11	74	theme	workflow	1987:1994	arg1	usage					1963:1967	the usage	1959:1967	the usage of the diagnostic workflow	1959:1994	Two patients with gastric cancer were selected to perform and demonstrate the usage of the diagnostic workflow.
31258789	15	75	from	presence	2358:2365	arg1	patients					2395:2402	patients	2395:2402	patients with advanced gastric cancer	2395:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	8	76	theme	neutral	1348:1354	arg1	glycosylation					1356:1368	neutral glycosylation	1348:1368	neutral glycosylation	1348:1368	Several differences in IgG glycosylation were found: sialylation and fucosylation were increased in peritoneal metastasis, while neutral glycosylation, monogalacosylation and bisecting GlcNAc were decreased.
31258789	3	77	theme	diagnosis	486:494	arg1	burden					466:471	the burden	462:471	the burden of operative diagnosis	462:494	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	0	78	theme	Preoperative	51:62	arg1	Discrimination					64:77	the Preoperative Discrimination	47:77	the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer	47:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	15	79	theme	peritoneal	2370:2379	arg1	metastasis					2381:2390	peritoneal metastasis	2370:2390	peritoneal metastasis	2370:2390	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	1	80	from	prognosis	190:198	arg1	cancer					211:216	gastric cancer	203:216	gastric cancer	203:216	Background: Peritoneal metastasis, associated with poor prognosis in gastric cancer, is difficult to discriminate from advanced gastric cancer preoperatively.
31258789	9	81	theme	advanced	1764:1771	arg1	cancer					1781:1786	advanced gastric cancer	1764:1786	advanced gastric cancer	1764:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	12	82	from	profiles	2046:2053	arg1	number					2066:2071	a large number	2058:2071	a large number of preoperative peritoneal metastasis serums	2058:2116	Conclusions: Here we firstly present IgG glycome profiles in a large number of preoperative peritoneal metastasis serums.
31258789	0	83	from	Value	4:8	arg1	Discrimination					64:77	the Preoperative Discrimination	47:77	the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer	47:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	0	83	from	Value	4:8	arg1	Cancer					126:131	Advanced Gastric Cancer	109:131	Advanced Gastric Cancer	109:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	4	84	theme	metastasis	725:734	arg1	discrimination					696:709	preoperative discrimination	683:709	preoperative discrimination of peritoneal metastasis from advanced gastric cancer	683:763	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	13	85	theme	IgG	2123:2125	arg1	glycan					2127:2132	The IgG glycan	2119:2132	The IgG glycan	2119:2132	The IgG glycan was highly associated with peritoneal metastasis.
31258789	0	86	theme	Metastasis	93:102	arg1	Discrimination					64:77	the Preoperative Discrimination	47:77	the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer	47:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	6	87	theme	liquid	1010:1015	arg1	chromatography					1017:1030	ultra-performance liquid chromatography	992:1030	ultra-performance liquid chromatography	992:1030	The IgG N-glycome composition was analyzed by ultra-performance liquid chromatography.
31258789	0	88	theme	Advanced	109:116	arg1	Cancer					126:131	Advanced Gastric Cancer	109:131	Advanced Gastric Cancer	109:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	5	89	theme	gastric	828:834	arg1	patients					843:850	373 gastric cancer patients	824:850	373 gastric cancer patients	824:850	Methods: A total of 373 gastric cancer patients were enrolled and randomly sorted into training cohort (n=249) and validation cohort (n=124).
31258789	15	90	with	patients	2395:2402	arg1	cancer					2426:2431	advanced gastric cancer	2409:2431	advanced gastric cancer	2409:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	11	91	theme	gastric	1903:1909	arg1	cancer					1911:1916	gastric cancer	1903:1916	gastric cancer	1903:1916	Two patients with gastric cancer were selected to perform and demonstrate the usage of the diagnostic workflow.
31258789	0	92	theme	Serum	13:17	arg1	Glycome					36:42	Serum Immunoglobulin G Glycome	13:42	Serum Immunoglobulin G Glycome	13:42	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	9	93	theme	curve	1617:1621	arg1	value					1629:1633	characteristic curve (AUC) value	1602:1633	characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer	1602:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	4	94	theme	comprehensive	778:790	arg1	set					799:801	the comprehensive sample set	774:801	the comprehensive sample set	774:801	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	3	95	theme	operative	476:484	arg1	diagnosis					486:494	operative diagnosis	476:494	operative diagnosis	476:494	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	0	96	theme	G	34:34	arg1	Glycome					36:42	Serum Immunoglobulin G Glycome	13:42	Serum Immunoglobulin G Glycome	13:42	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	0	97	from	Cancer	126:131	arg1	Discrimination					64:77	the Preoperative Discrimination	47:77	the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer	47:131	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	0	97	from	Cancer	126:131	arg1	Value					4:8	The Value	0:8	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.	0:132	The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer.
31258789	7	98	theme	metastasis	1167:1176	arg1	group					1178:1182	peritoneal metastasis group	1156:1182	peritoneal metastasis group	1156:1182	Results: Twenty-four glycan peaks were directly detected and 15 traits based on the same structures were evaluated between peritoneal metastasis group and advanced gastric cancer group.
31258789	9	99	dep	peaks	1509:1513	arg1	GP23					1541:1544	GP23	1541:1544	GP23	1541:1544	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	99	dep	peaks	1509:1513	arg1	GP21					1532:1535	GP21	1532:1535	GP21	1532:1535	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	99	dep	peaks	1509:1513	arg1	peaks					1509:1513	five glycan peaks	1497:1513	five glycan peaks (GP6, GP9, GP11, GP21 and GP23)	1497:1545	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	3	100	theme	survival	508:515	arg1	quality					517:523	survival quality	508:523	survival quality of patients	508:535	Consequently, a non-invasive biomarker is necessary to reduce the burden of operative diagnosis and improve survival quality of patients.
31258789	9	101	theme	significant	1440:1450	arg1	profile					1462:1468	the significant glycomics profile	1436:1468	the significant glycomics profile	1436:1468	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	102	theme	0.80	1638:1641	arg1	value					1629:1633	characteristic curve (AUC) value	1602:1633	characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer	1602:1786	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	10	103	theme	model	1824:1828	arg1	performance					1804:1814	The diagnostic performance	1789:1814	The diagnostic performance of this model	1789:1828	The diagnostic performance of this model was further validated in a combined cohort (AUC=0.79).
31258789	15	104	theme	presence	2358:2365	arg1	predictor					2341:2349	a reliable predictor	2330:2349	a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer	2330:2431	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	15	104	theme	presence	2358:2365	arg1	glyco-model					2309:2319	the newly established glyco-model	2287:2319	the newly established glyco-model	2287:2319	Besides, our results suggested that the newly established glyco-model could be a reliable predictor of the presence of peritoneal metastasis in patients with advanced gastric cancer.
31258789	9	105	theme	training	1644:1651	arg1	0.80					1638:1641	0.80	1638:1641	0.80	1638:1641	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	9	105	theme	training	1644:1651	arg1	cohort					1653:1658	training cohort	1644:1658	training cohort	1644:1658	Based on the significant glycomics profile, a glyco-model composed of five glycan peaks (GP6, GP9, GP11, GP21 and GP23) was established with area under the receiver operating characteristic curve (AUC) value of 0.80 (training cohort) and 0.77 (validation cohort), which showed good potential in discriminating peritoneal metastasis from advanced gastric cancer.
31258789	4	106	theme	peritoneal	628:637	arg1	metastasis					639:648	peritoneal metastasis	628:648	peritoneal metastasis	628:648	This study aims to elucidate the correlation between Immunoglobulin G (IgG) N-glycome and peritoneal metastasis and find potential biomarkers in preoperative discrimination of peritoneal metastasis from advanced gastric cancer based on the comprehensive sample set.
31258789	2	107	theme	economic	370:377	arg1	burden					379:384	economic burden	370:384	economic burden for patients	370:397	However, operative diagnosis could bring both mental and physical trauma and economic burden for patients.
31499104	6	0	theme	residues	869:876	arg1	O-6					848:850	position O-6	839:850	position O-6 of the branching residues	839:876	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	4	1	theme	molecular	281:289	arg1	weight					291:296	The average molecular weight	269:296	The average molecular weight of this polysaccharide	269:319	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	4	1	theme	molecular	281:289	arg1	1.5 × 104 Da					341:352	1.5 × 104 Da	341:352	1.5 × 104 Da	341:352	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	6	2	theme	periodate	501:509	arg1	oxidation					511:519	periodate oxidation	501:519	periodate oxidation	501:519	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	0	3	from	elucidation	10:20	arg1	spores					79:84	spores	79:84	spores of Ganoderma lucidum	79:105	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	6	4	theme	terminal	776:783	arg1	residue					816:822	terminal and (1 → 6)-β-d-glucopyranosyl residue	776:822	residue	816:822	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	5	theme	-β-d-glucopyranosyl	796:814	arg1	residue					816:822	terminal and (1 → 6)-β-d-glucopyranosyl residue	776:822	residue	816:822	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	6	theme	β-d-glucose	586:596	arg1	residue					598:604	β-d-glucose residue	586:604	β-d-glucose residue	586:604	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	0	7	theme	Ganoderma	89:97	arg1	lucidum					99:105	Ganoderma lucidum	89:105	Ganoderma lucidum	89:105	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	7	8	theme	in	906:907	arg1	assays					899:904	Anti-tumor activity assays	879:904	Anti-tumor activity assays in vivo	879:912	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	7	9	dep	in	906:907	arg1	vivo					909:912	vivo	909:912	vivo	909:912	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	9	10	theme	potential	1092:1100	arg1	WGLP					1076:1079	WGLP	1076:1079	WGLP	1076:1079	It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.
31499104	9	10	theme	potential	1092:1100	arg1	agent					1113:1117	a potential anti-tumor agent	1090:1117	a potential anti-tumor agent used in the clinic	1090:1136	It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.
31499104	2	11	theme	new	131:133	arg1	constituents					146:157	new anti-tumor constituents	131:157	new anti-tumor constituents	131:157	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	2	12	theme	lucidum	248:254	arg1	spores					228:233	spores	228:233	spores of Ganoderma lucidum	228:254	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	0	13	theme	lucidum	99:105	arg1	spores					79:84	spores	79:84	spores of Ganoderma lucidum	79:105	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	6	14	theme	position	839:846	arg1	O-6					848:850	position O-6	839:850	position O-6 of the branching residues	839:876	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	15	theme	side	751:754	arg1	chain					756:760	the side chain	747:760	the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue	747:822	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	2	16	theme	Ganoderma	238:246	arg1	lucidum					248:254	Ganoderma lucidum	238:254	Ganoderma lucidum	238:254	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	2	17	dep	obtained	214:221	arg1	Fr					257:258	Fr	257:258	Fr.	257:259	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	0	18	from	activity	37:44	arg1	spores					79:84	spores	79:84	spores of Ganoderma lucidum	79:105	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	8	19	theme	toxic	1023:1027	arg1	reactions					1029:1037	no drug-related toxic reactions	1007:1037	no drug-related toxic reactions	1007:1037	Furthermore, no drug-related toxic reactions were observed.
31499104	6	20	theme	Smith	525:529	arg1	degradation					531:541	Smith degradation	525:541	Smith degradation	525:541	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	21	theme	branching	859:867	arg1	residues					869:876	the branching residues	855:876	the branching residues	855:876	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	0	22	theme	Structure	0:8	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	8	23	theme	drug-related	1010:1021	arg1	reactions					1029:1037	no drug-related toxic reactions	1007:1037	no drug-related toxic reactions	1007:1037	Furthermore, no drug-related toxic reactions were observed.
31499104	7	24	theme	Anti-tumor	879:888	arg1	assays					899:904	Anti-tumor activity assays	879:904	Anti-tumor activity assays in vivo	879:912	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	6	25	theme	hexasaccharide	653:666	arg1	unit					645:648	an average repeating unit	624:648	an average repeating unit of hexasaccharide	624:666	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	0	26	theme	anti-tumor	26:35	arg1	activity					37:44	anti-tumor activity	26:44	anti-tumor activity in vivo	26:52	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	6	27	theme	13C	475:477	arg1	spectroscopy					483:494	13C NMR spectroscopy	475:494	13C NMR spectroscopy	475:494	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	7	28	theme	activity	890:897	arg1	assays					899:904	Anti-tumor activity assays	879:904	Anti-tumor activity assays in vivo	879:912	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	7	29	theme	S180	963:966	arg1	growth					974:979	the S180 tumor growth	959:979	the S180 tumor growth in the mice	959:991	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	0	30	from	spores	79:84	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	0	30	from	spores	79:84	arg1	activity					37:44	anti-tumor activity	26:44	anti-tumor activity in vivo	26:52	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	0	30	from	spores	79:84	arg1	polysaccharide					59:72	a polysaccharide	57:72	a polysaccharide from spores of Ganoderma lucidum	57:105	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	6	31	dep	terminal	776:783	arg1	1 → 6					790:794	1 → 6	790:794	1 → 6	790:794	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	32	theme	repeating	635:643	arg1	unit					645:648	an average repeating unit	624:648	an average repeating unit of hexasaccharide	624:666	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	7	33	from	growth	974:979	arg1	mice					988:991	the mice	984:991	the mice	984:991	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	6	34	theme	NMR	479:481	arg1	spectroscopy					483:494	13C NMR spectroscopy	475:494	13C NMR spectroscopy	475:494	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	35	contain	contained	576:584	arg2	residue					598:604	β-d-glucose residue	586:604	β-d-glucose residue	586:604	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	35	contain	contained	576:584	arg1	WGLP					571:574	WGLP	571:574	WGLP	571:574	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	2	36	theme	anti-tumor	135:144	arg1	constituents					146:157	new anti-tumor constituents	131:157	new anti-tumor constituents	131:157	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	6	37	theme	methylation	446:456	arg1	analysis					458:465	methylation analysis	446:465	methylation analysis	446:465	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	2	38	theme	water-soluble	175:187	arg1	WGLP					205:208	WGLP	205:208	WGLP	205:208	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	2	38	theme	water-soluble	175:187	arg1	polysaccharide					189:202	a water-soluble polysaccharide	173:202	a water-soluble polysaccharide	173:202	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.)
31499104	6	39	theme	-β-d-glucopyranosyl	708:726	arg1	residues					728:735	(1 → 3)-β-d-glucopyranosyl residues	701:735	(1 → 3)-β-d-glucopyranosyl residues	701:735	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	40	theme	average	627:633	arg1	unit					645:648	an average repeating unit	624:648	an average repeating unit of hexasaccharide	624:666	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	4	41	theme	polysaccharide	306:319	arg1	weight					291:296	The average molecular weight	269:296	The average molecular weight of this polysaccharide	269:319	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	4	41	theme	polysaccharide	306:319	arg1	1.5 × 104 Da					341:352	1.5 × 104 Da	341:352	1.5 × 104 Da	341:352	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	7	42	theme	tumor	968:972	arg1	growth					974:979	the S180 tumor growth	959:979	the S180 tumor growth in the mice	959:991	Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice.
31499104	4	43	theme	average	273:279	arg1	weight					291:296	The average molecular weight	269:296	The average molecular weight of this polysaccharide	269:319	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	4	43	theme	average	273:279	arg1	1.5 × 104 Da					341:352	1.5 × 104 Da	341:352	1.5 × 104 Da	341:352	The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da.
31499104	5	44	theme	Monosaccharide	355:368	arg1	analysis					382:389	Monosaccharide composition analysis	355:389	Monosaccharide composition analysis	355:389	Monosaccharide composition analysis indicated that it was composed solely of glucose.
31499104	0	45	theme	polysaccharide	59:72	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	0	45	theme	polysaccharide	59:72	arg1	activity					37:44	anti-tumor activity	26:44	anti-tumor activity in vivo	26:52	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	0	46	dep	elucidation	10:20	arg1	Fr					108:109	Fr	108:109	Fr.	108:110	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.)
31499104	9	47	theme	anti-tumor	1102:1111	arg1	WGLP					1076:1079	WGLP	1076:1079	WGLP	1076:1079	It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.
31499104	9	47	theme	anti-tumor	1102:1111	arg1	agent					1113:1117	a potential anti-tumor agent	1090:1117	a potential anti-tumor agent used in the clinic	1090:1136	It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.
31499104	5	48	theme	composition	370:380	arg1	analysis					382:389	Monosaccharide composition analysis	355:389	Monosaccharide composition analysis	355:389	Monosaccharide composition analysis indicated that it was composed solely of glucose.
31499104	6	49	attach	attached	827:834	arg3	O-6					848:850	position O-6	839:850	position O-6 of the branching residues	839:876	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	49	attach	attached	827:834	arg2	chain					756:760	the side chain	747:760	the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue	747:822	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
31499104	6	49	attach	attached	827:834	arg1	backbone					678:685	a backbone	676:685	a backbone	676:685	From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues.
30261252	4	0	theme	%	613:613	arg1	fructose					615:622	16.15% fructose	608:622	16.15% fructose	608:622	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	5	1	theme	Morphological	670:682	arg1	study					684:688	Morphological study	670:688	Morphological study by scanning electron microscopy	670:720	Morphological study by scanning electron microscopy demonstrated less porous flakes like structure.
30261252	9	2	theme	EPS	1279:1281	arg1	nature					1269:1274	concentration-dependent water soluble nature	1231:1274	concentration-dependent water soluble nature of EPS	1231:1281	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	7	3	theme	X-ray	909:913	arg1	pattern					927:933	The X-ray diffraction pattern	905:933	The X-ray diffraction pattern	905:933	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	8	4	theme	gravimetric	1036:1046	arg1	TGA					1058:1060	TGA	1058:1060	TGA	1058:1060	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	8	4	theme	gravimetric	1036:1046	arg1	analysis					1048:1055	Thermal gravimetric analysis	1028:1055	Thermal gravimetric analysis (TGA)	1028:1061	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	11	5	theme	in-vitro	1503:1510	arg1	activity					1523:1530	in-vitro anti-tumor activity	1503:1530	in-vitro anti-tumor activity	1503:1530	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	9	6	theme	good	1288:1291	arg1	water					1293:1297	good water	1288:1297	good water (98.8%)	1288:1305	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	9	6	theme	good	1288:1291	arg1	%					1304:1304	98.8%	1300:1304	98.8%	1300:1304	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	2	7	theme	first	313:317	arg1	report					319:324	the first report	309:324	the first report to reveal its structural-functional properties and biological activities	309:397	This is the first report to reveal its structural-functional properties and biological activities.
30261252	2	7	theme	first	313:317	arg1	This					301:304	This	301:304	This	301:304	This is the first report to reveal its structural-functional properties and biological activities.
30261252	10	8	dep	oils	1444:1447	arg1	all					1425:1427	all	1425:1427	all	1425:1427	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
30261252	3	9	with	composition	481:491	arg1	HPLC					506:509	HPLC	506:509	HPLC	506:509	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	3	9	with	composition	481:491	arg1	TLC					498:500	TLC	498:500	TLC	498:500	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	7	10	dep	pattern	927:933	arg1	observations					980:991	observations	980:991	observations	980:991	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	8	11	theme	rheological	1067:1077	arg1	studies					1079:1085	rheological studies	1067:1085	rheological studies	1067:1085	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	4	12	theme	%	649:649	arg1	acid					664:667	28.18% galacturonic acid	644:667	28.18% galacturonic acid	644:667	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	5	13	theme	porous	740:745	arg1	flakes					747:752	less porous flakes	735:752	less porous flakes	735:752	Morphological study by scanning electron microscopy demonstrated less porous flakes like structure.
30261252	0	14	from	PASS26	108:113	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	14	from	PASS26	108:113	arg1	characterization					39:54	structural-functional characterization	17:54	structural-functional characterization	17:54	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	14	from	PASS26	108:113	arg1	exopolysaccharide					62:78	an exopolysaccharide	59:78	an exopolysaccharide from Bacillus licheniformis PASS26	59:113	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	1	15	theme	salt	245:248	arg1	pan					250:252	a salt pan	243:252	a salt pan inhabiting bacteria	243:272	The exopolysaccharide (EPS) synthesized by Bacillus licheniformis PASS26, a salt pan inhabiting bacteria was successfully purified.
30261252	1	15	theme	salt	245:248	arg1	PASS26					235:240	PASS26	235:240	PASS26	235:240	The exopolysaccharide (EPS) synthesized by Bacillus licheniformis PASS26, a salt pan inhabiting bacteria was successfully purified.
30261252	5	16	theme	electron	702:709	arg1	microscopy					711:720	scanning electron microscopy	693:720	scanning electron microscopy	693:720	Morphological study by scanning electron microscopy demonstrated less porous flakes like structure.
30261252	9	17	theme	holding	1324:1330	arg1	capacity					1332:1339	holding capacity	1324:1339	holding capacity	1324:1339	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	12	18	theme	pharmaceutical	1661:1674	arg1	industries					1676:1685	food and pharmaceutical industries	1652:1685	food and pharmaceutical industries	1652:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	3	19	theme	low	404:406	arg1	56 kDa					426:431	56 kDa	426:431	56 kDa	426:431	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	3	19	theme	low	404:406	arg1	weight					418:423	low molecular weight	404:423	The low molecular weight (56 kDa) polysaccharide	400:447	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	0	20	from	Purification	0:11	arg1	PASS26					108:113	PASS26	108:113	PASS26	108:113	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	21	theme	in-vitro	120:127	arg1	antitumor					129:137	in-vitro antitumor	120:137	in-vitro antitumor	120:137	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	8	22	theme	thermal	1106:1112	arg1	stability					1114:1122	moderate thermal stability	1097:1122	moderate thermal stability	1097:1122	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	7	23	theme	semi-crystalline	1003:1018	arg1	nature					1020:1025	semi-crystalline nature	1003:1025	semi-crystalline nature	1003:1025	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	10	24	theme	emulsification	1368:1381	arg1	activity					1383:1390	moderate emulsification activity	1359:1390	moderate emulsification activity	1359:1390	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
30261252	12	25	theme	food	1652:1655	arg1	industries					1676:1685	food and pharmaceutical industries	1652:1685	food and pharmaceutical industries	1652:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	11	26	theme	wound	1536:1540	arg1	healing					1542:1548	wound healing	1536:1548	wound healing	1536:1548	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	11	27	theme	activity	1523:1530	arg1	efficiency					1550:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	3	28	theme	monosaccharide	466:479	arg1	composition					481:491	monosaccharide composition	466:491	monosaccharide composition with TLC and HPLC	466:509	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	12	29	from	applications	1636:1647	arg1	industries					1676:1685	food and pharmaceutical industries	1652:1685	food and pharmaceutical industries	1652:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	3	30	theme	weight	418:423	arg1	polysaccharide					434:447	The low molecular weight (56 kDa) polysaccharide	400:447	The low molecular weight (56 kDa) polysaccharide	400:447	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	4	31	theme	9.89	591:594	arg1	%					595:595	%	595:595	%	595:595	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	0	32	theme	structural-functional	17:37	arg1	characterization					39:54	structural-functional characterization	17:54	structural-functional characterization	17:54	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	8	33	theme	30-350 °C	1140:1148	arg1	range					1131:1135	a range	1129:1135	a range of 30-350 °C and semi-viscous nature	1129:1172	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	9	34	from	Studies	1188:1194	arg1	properties					1210:1219	functional properties	1199:1219	functional properties	1199:1219	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	10	35	theme	moderate	1359:1366	arg1	activity					1383:1390	moderate emulsification activity	1359:1390	moderate emulsification activity	1359:1390	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
30261252	8	36	theme	semi-viscous	1154:1165	arg1	nature					1167:1172	semi-viscous nature	1154:1172	semi-viscous nature	1154:1172	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	12	37	theme	potential	1626:1634	arg1	applications					1636:1647	potential applications	1626:1647	potential applications in food and pharmaceutical industries	1626:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	4	38	theme	18.44	575:579	arg1	%					580:580	%	580:580	%	580:580	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	0	39	theme	exopolysaccharide	62:78	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	39	theme	exopolysaccharide	62:78	arg1	characterization					39:54	structural-functional characterization	17:54	structural-functional characterization	17:54	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	12	40	theme	significant	1573:1583	arg1	properties					1611:1620	significant functional and biological properties	1573:1620	significant functional and biological properties for potential applications in food and pharmaceutical industries	1573:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	6	41	theme	polysaccharide	889:902	arg1	nature					875:880	a partially charged nature	855:880	a partially charged nature of the polysaccharide	855:902	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	10	42	theme	excellent	1397:1405	arg1	stability					1407:1415	excellent stability	1397:1415	excellent stability against all the food grade oils and hydrocarbons tested	1397:1471	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
30261252	6	43	theme	charged	867:873	arg1	nature					875:880	a partially charged nature	855:880	a partially charged nature of the polysaccharide	855:902	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	9	44	theme	functional	1199:1208	arg1	properties					1210:1219	functional properties	1199:1219	functional properties	1199:1219	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	4	45	theme	27.32	625:629	arg1	%					630:630	%	630:630	%	630:630	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	10	46	theme	grade	1438:1442	arg1	oils					1444:1447	the food grade oils	1429:1447	the food grade oils	1429:1447	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
30261252	9	47	theme	soluble	1261:1267	arg1	nature					1269:1274	concentration-dependent water soluble nature	1231:1274	concentration-dependent water soluble nature of EPS	1231:1281	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	7	48	theme	scanning	952:959	arg1	DSC					975:977	DSC	975:977	DSC	975:977	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	7	48	theme	scanning	952:959	arg1	calorimetric					961:972	differential scanning calorimetric	939:972	differential scanning calorimetric (DSC)	939:978	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	8	49	theme	Thermal	1028:1034	arg1	TGA					1058:1060	TGA	1058:1060	TGA	1058:1060	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	8	49	theme	Thermal	1028:1034	arg1	analysis					1048:1055	Thermal gravimetric analysis	1028:1055	Thermal gravimetric analysis (TGA)	1028:1061	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	4	50	theme	16.15	608:612	arg1	%					613:613	%	613:613	%	613:613	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	11	51	theme	interesting	1491:1501	arg1	efficiency					1550:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	7	52	theme	diffraction	915:925	arg1	pattern					927:933	The X-ray diffraction pattern	905:933	The X-ray diffraction pattern	905:933	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	6	53	theme	nitrogen	834:841	arg1	quantity					822:829	a small quantity	814:829	a small quantity of nitrogen	814:841	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	6	54	theme	quantity	822:829	arg1	presence					802:809	the presence	798:809	the presence of a small quantity of nitrogen	798:841	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	2	55	theme	structural-functional	340:360	arg1	properties					362:371	its structural-functional properties	336:371	its structural-functional properties	336:371	This is the first report to reveal its structural-functional properties and biological activities.
30261252	6	56	theme	small	816:820	arg1	quantity					822:829	a small quantity	814:829	a small quantity of nitrogen	814:841	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	5	57	theme	less	735:738	arg1	flakes					747:752	less porous flakes	735:752	less porous flakes	735:752	Morphological study by scanning electron microscopy demonstrated less porous flakes like structure.
30261252	4	58	theme	28.18	644:648	arg1	%					649:649	%	649:649	%	649:649	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	4	59	theme	galacturonic	651:662	arg1	acid					664:667	28.18% galacturonic acid	644:667	28.18% galacturonic acid	644:667	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	5	60	theme	scanning	693:700	arg1	microscopy					711:720	scanning electron microscopy	693:720	scanning electron microscopy	693:720	Morphological study by scanning electron microscopy demonstrated less porous flakes like structure.
30261252	4	61	theme	EPS	566:568	arg1	nature					556:561	the hetero-polymeric nature	535:561	the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid	535:667	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	9	62	dep	water	1293:1297	arg1	capacity					1332:1339	holding capacity	1324:1339	holding capacity	1324:1339	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	0	63	with	Purification	0:11	arg1	activities					157:166	in-vitro antitumor and wound healing activities	120:166	in-vitro antitumor and wound healing activities	120:166	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	64	from	characterization	39:54	arg1	PASS26					108:113	PASS26	108:113	PASS26	108:113	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	8	65	theme	moderate	1097:1104	arg1	stability					1114:1122	moderate thermal stability	1097:1122	moderate thermal stability	1097:1122	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	0	66	theme	healing	149:155	arg1	activities					157:166	in-vitro antitumor and wound healing activities	120:166	in-vitro antitumor and wound healing activities	120:166	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	0	67	theme	antitumor	129:137	arg1	activities					157:166	in-vitro antitumor and wound healing activities	120:166	in-vitro antitumor and wound healing activities	120:166	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	3	68	theme	molecular	408:416	arg1	56 kDa					426:431	56 kDa	426:431	56 kDa	426:431	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	3	68	theme	molecular	408:416	arg1	weight					418:423	low molecular weight	404:423	The low molecular weight (56 kDa) polysaccharide	400:447	The low molecular weight (56 kDa) polysaccharide was analyzed for monosaccharide composition with TLC and HPLC.
30261252	0	69	theme	wound	143:147	arg1	healing					149:155	wound healing	143:155	wound healing	143:155	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	4	70	theme	hetero-polymeric	539:554	arg1	nature					556:561	the hetero-polymeric nature	535:561	the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid	535:667	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	11	71	theme	anti-tumor	1512:1521	arg1	activity					1523:1530	in-vitro anti-tumor activity	1503:1530	in-vitro anti-tumor activity	1503:1530	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	4	72	theme	%	595:595	arg1	galactose					597:605	9.89% galactose	591:605	9.89% galactose	591:605	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	6	73	theme	Elemental	770:778	arg1	analysis					780:787	Elemental analysis	770:787	Elemental analysis	770:787	Elemental analysis revealed the presence of a small quantity of nitrogen, indicating a partially charged nature of the polysaccharide.
30261252	11	74	theme	healing	1542:1548	arg1	efficiency					1550:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	interesting in-vitro anti-tumor activity and wound healing efficiency	1491:1559	Studies revealed interesting in-vitro anti-tumor activity and wound healing efficiency.
30261252	8	75	theme	nature	1167:1172	arg1	range					1131:1135	a range	1129:1135	a range of 30-350 °C and semi-viscous nature	1129:1172	Thermal gravimetric analysis (TGA) and rheological studies displayed moderate thermal stability over a range of 30-350 °C and semi-viscous nature respectively.
30261252	12	76	theme	biological	1600:1609	arg1	properties					1611:1620	significant functional and biological properties	1573:1620	significant functional and biological properties for potential applications in food and pharmaceutical industries	1573:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	9	77	theme	concentration-dependent	1231:1253	arg1	nature					1269:1274	concentration-dependent water soluble nature	1231:1274	concentration-dependent water soluble nature of EPS	1231:1281	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	4	78	theme	%	580:580	arg1	glucose					582:588	18.44% glucose	575:588	18.44% glucose	575:588	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	0	79	with	characterization	39:54	arg1	activities					157:166	in-vitro antitumor and wound healing activities	120:166	in-vitro antitumor and wound healing activities	120:166	Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities.
30261252	2	80	theme	biological	377:386	arg1	activities					388:397	biological activities	377:397	biological activities	377:397	This is the first report to reveal its structural-functional properties and biological activities.
30261252	4	81	theme	%	630:630	arg1	mannose					632:638	27.32% mannose	625:638	27.32% mannose	625:638	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	9	82	theme	water	1255:1259	arg1	nature					1269:1274	concentration-dependent water soluble nature	1231:1274	concentration-dependent water soluble nature of EPS	1231:1281	Studies on functional properties displayed concentration-dependent water soluble nature of EPS with good water (98.8%) and oil (101.7%) holding capacity.
30261252	4	83	with	nature	556:561	arg1	fructose					615:622	16.15% fructose	608:622	16.15% fructose	608:622	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	4	83	with	nature	556:561	arg1	mannose					632:638	27.32% mannose	625:638	27.32% mannose	625:638	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	4	83	with	nature	556:561	arg1	acid					664:667	28.18% galacturonic acid	644:667	28.18% galacturonic acid	644:667	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	4	83	with	nature	556:561	arg1	galactose					597:605	9.89% galactose	591:605	9.89% galactose	591:605	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	4	83	with	nature	556:561	arg1	glucose					582:588	18.44% glucose	575:588	18.44% glucose	575:588	The analyses confirmed the hetero-polymeric nature of EPS with 18.44% glucose, 9.89% galactose, 16.15% fructose, 27.32% mannose and 28.18% galacturonic acid.
30261252	7	84	theme	differential	939:950	arg1	DSC					975:977	DSC	975:977	DSC	975:977	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	7	84	theme	differential	939:950	arg1	calorimetric					961:972	differential scanning calorimetric	939:972	differential scanning calorimetric (DSC)	939:978	The X-ray diffraction pattern and differential scanning calorimetric (DSC) observations reflected semi-crystalline nature.
30261252	12	85	theme	functional	1585:1594	arg1	properties					1611:1620	significant functional and biological properties	1573:1620	significant functional and biological properties for potential applications in food and pharmaceutical industries	1573:1685	EPS showed significant functional and biological properties for potential applications in food and pharmaceutical industries.
30261252	10	86	theme	food	1433:1436	arg1	oils					1444:1447	the food grade oils	1429:1447	the food grade oils	1429:1447	The EPS acquired moderate emulsification activity with excellent stability against all the food grade oils and hydrocarbons tested.
31499114	7	0	theme	vinyl	1350:1354	arg1	both					1365:1368	both	1365:1368	both	1365:1368	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	7	0	theme	vinyl	1350:1354	arg1	polymer					1356:1362	the vinyl polymer	1346:1362	the vinyl polymer	1346:1362	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	4	1	theme	X-ray	823:827	arg1	diffractometry					829:842	X-ray diffractometry	823:842	X-ray diffractometry	823:842	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	9	2	theme	CNC/polymer	1961:1971	arg1	composites					1973:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	9	3	theme	composites	1973:1982	arg1	performance					1890:1900	the thermal performance	1878:1900	the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites	1878:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	9	3	theme	composites	1973:1982	arg1	resistance					1914:1923	heat/flame resistance	1903:1923	heat/flame resistance	1903:1923	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	8	4	theme	nematic	1530:1536	arg1	organization					1538:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	2	5	dep	P	550:550	arg1	AA = 95:5-70:30					570:584	AA = 95:5-70:30	570:584	AA = 95:5-70:30	570:584	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	7	6	theme	AA	1335:1336	arg1	unit					1338:1341	the AA unit	1331:1341	the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-),	1331:1411	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	7	7	from	CNC	1323:1325	arg1	both					1365:1368	both	1365:1368	both	1365:1368	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	7	7	from	CNC	1323:1325	arg1	polymer					1356:1362	the vinyl polymer	1346:1362	the vinyl polymer	1346:1362	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	4	8	theme	time	873:876	arg1	Polymorphism					758:769	Polymorphism	758:769	Polymorphism of CaCO3 deposited inside the films	758:805	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	4	8	theme	time	873:876	arg1	function					849:856	a function	847:856	a function of the soaking time (1-5 day) and also of the matrix composition	847:921	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	8	9	theme	mineralized	1574:1584	arg1	films					1586:1590	the mineralized films	1570:1590	the mineralized films	1570:1590	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	7	10	from	unit	1338:1341	arg1	both					1365:1368	both	1365:1368	both	1365:1368	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	7	10	from	unit	1338:1341	arg1	polymer					1356:1362	the vinyl polymer	1346:1362	the vinyl polymer	1346:1362	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	8	11	theme	ionic	1737:1741	arg1	strength					1743:1750	ionic strength	1737:1750	ionic strength	1737:1750	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	1	12	theme	nematic	228:234	arg1	structure					236:244	a chiral nematic structure	219:244	a chiral nematic structure of CNC assembly	219:260	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	3	13	contain	containing	694:703	arg2	Ca2+					705:708	Ca2+	705:708	Ca2+	705:708	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	3	13	contain	containing	694:703	arg1	solution					685:692	a salt solution	678:692	a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9)	678:755	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	3	13	contain	containing	694:703	arg2	HCO3-					714:718	HCO3-	714:718	HCO3-	714:718	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	5	14	theme	amorphous	983:991	arg1	soaking					1024:1030	1-day soaking	1018:1030	1-day soaking	1018:1030	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	14	theme	amorphous	983:991	arg1	ACC					1012:1014	ACC	1012:1014	ACC	1012:1014	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	14	theme	amorphous	983:991	arg1	carbonate					1001:1009	amorphous calcium carbonate	983:1009	amorphous calcium carbonate (ACC) (1-day soaking)	983:1031	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	1	15	theme	CNC	249:251	arg1	assembly					253:260	CNC assembly	249:260	CNC assembly	249:260	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	4	16	theme	matrix	904:909	arg1	composition					911:921	the matrix composition	900:921	the matrix composition	900:921	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	1	17	theme	CNC/vinyl	344:352	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	17	theme	CNC/vinyl	344:352	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	18	theme	cellulose	164:172	arg1	CNC					187:189	CNC	187:189	CNC	187:189	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	18	theme	cellulose	164:172	arg1	nanocrystal					174:184	cellulose nanocrystal	164:184	cellulose nanocrystal (CNC)	164:190	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	0	19	theme	chiral	83:88	arg1	structure					110:118	a chiral nematic mesomorphic structure	81:118	a chiral nematic mesomorphic structure	81:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	1	20	theme	lyotropics	362:371	arg1	process					310:316	a polymerization process	293:316	a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	293:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	2	21	theme	methacrylate-co-acrylic	519:541	arg1	acid					543:546	2-hydroxyethyl methacrylate-co-acrylic acid	504:546	2-hydroxyethyl methacrylate-co-acrylic acid	504:546	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	2	21	theme	methacrylate-co-acrylic	519:541	arg1	CNC/poly					495:502	CNC/poly	495:502	CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol)	495:592	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	8	22	theme	wet	1797:1799	arg1	process					1801:1807	the wet process	1793:1807	the wet process	1793:1807	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	1	23	theme	7-11 wt	374:380	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	23	theme	7-11 wt	374:380	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	6	24	theme	stable	1221:1226	arg1	calcite					1228:1234	stable calcite	1221:1234	stable calcite	1221:1234	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	6	25	from	prominent	1240:1248	arg1	mineralization					1156:1169	the mineralization	1152:1169	the mineralization of the CNC/P(HEMA-co-AA) series	1152:1201	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	7	26	from	deposition	1467:1476	arg1	matrix					1498:1503	the swollen film matrix	1481:1503	the swollen film matrix	1481:1503	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	8	27	dep	kept	1555:1558	arg1	dried					1593:1597	dried	1593:1597	dried	1593:1597	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	9	28	theme	thermal	1882:1888	arg1	performance					1890:1900	the thermal performance	1878:1900	the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites	1878:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	9	28	theme	thermal	1882:1888	arg1	resistance					1914:1923	heat/flame resistance	1903:1923	heat/flame resistance	1903:1923	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	1	29	dep	using	158:162	arg1	wherein					211:217	wherein	211:217	wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	211:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	2	30	theme	composites	419:428	arg1	series					401:406	Two series	397:406	Two series of polymer composites	397:428	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	1	31	theme	polymerization	295:308	arg1	process					310:316	a polymerization process	293:316	a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	293:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	2	32	dep	CNC/poly	495:502	arg1	HEMA-co-AA					552:561	HEMA-co-AA	552:561	HEMA-co-AA	552:561	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	2	32	dep	CNC/poly	495:502	arg1	HEMA					565:568	HEMA	565:568	HEMA	565:568	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	2	32	dep	CNC/poly	495:502	arg1	P					550:550	P	550:550	P(HEMA-co-AA)	550:562	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	1	33	theme	CaCO3	121:125	arg1	mineralization					127:140	CaCO3 mineralization	121:140	CaCO3 mineralization	121:140	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	7	34	theme	swollen	1485:1491	arg1	matrix					1498:1503	the swollen film matrix	1481:1503	the swollen film matrix	1481:1503	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	2	35	from	AA = 95:5-70:30	570:584	arg1	mol					589:591	mol	589:591	mol	589:591	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	4	36	theme	CaCO3	774:778	arg1	deposited					780:788	CaCO3 deposited	774:788	CaCO3 deposited inside the films	774:805	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	2	37	theme	2-hydroxyethyl	454:467	arg1	CNC/poly					445:452	CNC/poly	445:452	CNC/poly(2-hydroxyethyl methacrylate) (PHEMA)	445:489	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	2	37	theme	2-hydroxyethyl	454:467	arg1	methacrylate					469:480	2-hydroxyethyl methacrylate	454:480	2-hydroxyethyl methacrylate	454:480	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	6	38	theme	vaterite	1278:1285	arg1	appearance					1264:1273	minor appearance	1258:1273	minor appearance of vaterite	1258:1285	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	1	39	theme	precursory	325:334	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	39	theme	precursory	325:334	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	5	40	theme	aragonite	1122:1130	arg1	mixture					1098:1104	a mixture	1096:1104	a mixture of vaterite and aragonite (5-day soaking)	1096:1146	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	9	41	theme	mesomorphic	1933:1943	arg1	composites					1973:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	7	42	dep	group	1389:1393	arg1	-COO-					1405:1409	-COO-	1405:1409	-COO-	1405:1409	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	7	42	dep	group	1389:1393	arg1	-SO3-					1396:1400	-SO3-	1396:1400	-SO3-	1396:1400	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	8	43	from	increase	1725:1732	arg1	surroundings					1765:1776	the CNCs' surroundings	1755:1776	the CNCs' surroundings accompanied by the wet process	1755:1807	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	6	44	theme	minor	1258:1262	arg1	appearance					1264:1273	minor appearance	1258:1273	minor appearance of vaterite	1258:1285	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	6	45	theme	CNC/P	1178:1182	arg1	series					1196:1201	the CNC/P(HEMA-co-AA) series	1174:1201	the CNC/P(HEMA-co-AA) series	1174:1201	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	0	46	with	composites	32:41	arg1	nanocrystals					58:69	cellulose nanocrystals	48:69	cellulose nanocrystals providing a chiral nematic mesomorphic structure	48:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	5	47	theme	metastable	1036:1045	arg1	soaking					1075:1081	3-day soaking	1069:1081	3-day soaking	1069:1081	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	47	theme	metastable	1036:1045	arg1	vaterite					1059:1066	metastable crystalline vaterite	1036:1066	metastable crystalline vaterite (3-day soaking)	1036:1082	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	3	48	theme	composite	659:667	arg1	films					669:673	the composite films	655:673	the composite films	655:673	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	8	49	theme	chiral	1523:1528	arg1	organization					1538:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	5	50	theme	vaterite	1109:1116	arg1	mixture					1098:1104	a mixture	1096:1104	a mixture of vaterite and aragonite (5-day soaking)	1096:1146	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	7	51	theme	mesofiller	1312:1321	arg1	CNC					1323:1325	the mesofiller CNC	1308:1325	the mesofiller CNC	1308:1325	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	8	52	theme	attributable	1705:1716	arg1	mineralization					1689:1702	the mineralization	1685:1702	the mineralization	1685:1702	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	0	53	theme	cellulose	48:56	arg1	nanocrystals					58:69	cellulose nanocrystals	48:69	cellulose nanocrystals providing a chiral nematic mesomorphic structure	48:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	3	54	theme	salt	680:683	arg1	solution					685:692	a salt solution	678:692	a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9)	678:755	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	1	55	theme	/polymer	191:198	arg1	composites					200:209	/polymer composites	191:209	/polymer composites	191:209	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	5	56	theme	crystalline	1047:1057	arg1	soaking					1075:1081	3-day soaking	1069:1081	3-day soaking	1069:1081	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	56	theme	crystalline	1047:1057	arg1	vaterite					1059:1066	metastable crystalline vaterite	1036:1066	metastable crystalline vaterite (3-day soaking)	1036:1082	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	7	57	theme	CaCO3	1461:1465	arg1	deposition					1467:1476	the CaCO3 deposition	1457:1476	the CaCO3 deposition in the swollen film matrix	1457:1503	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	4	58	theme	soaking	865:871	arg1	time					873:876	the soaking time	861:876	the soaking time (1-5 day)	861:886	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	4	58	theme	soaking	865:871	arg1	1-5 day					879:885	1-5 day	879:885	1-5 day	879:885	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	8	59	theme	strength	1743:1750	arg1	increase					1725:1732	the increase	1721:1732	the increase of ionic strength in the CNCs' surroundings accompanied by the wet process	1721:1807	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	1	60	theme	chiral	221:226	arg1	structure					236:244	a chiral nematic structure	219:244	a chiral nematic structure of CNC assembly	219:260	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	6	61	theme	series	1196:1201	arg1	mineralization					1156:1169	the mineralization	1152:1169	the mineralization of the CNC/P(HEMA-co-AA) series	1152:1201	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	3	62	theme	low-basic	728:736	arg1	pH ≤ 9					749:754	pH ≤ 9	749:754	pH ≤ 9	749:754	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	3	62	theme	low-basic	728:736	arg1	condition					738:746	a low-basic condition	726:746	a low-basic condition (pH ≤ 9)	726:755	The mineralization was allowed to proceed solely by soaking the composite films in a salt solution containing Ca2+ and HCO3- under a low-basic condition (pH ≤ 9).
31499114	6	63	theme	HEMA-co-AA	1184:1193	arg1	series					1196:1201	the CNC/P(HEMA-co-AA) series	1174:1201	the CNC/P(HEMA-co-AA) series	1174:1201	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	5	64	theme	calcium	993:999	arg1	soaking					1024:1030	1-day soaking	1018:1030	1-day soaking	1018:1030	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	64	theme	calcium	993:999	arg1	ACC					1012:1014	ACC	1012:1014	ACC	1012:1014	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	64	theme	calcium	993:999	arg1	carbonate					1001:1009	amorphous calcium carbonate	983:1009	amorphous calcium carbonate (ACC) (1-day soaking)	983:1031	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	0	65	theme	mesomorphic	98:108	arg1	structure					110:118	a chiral nematic mesomorphic structure	81:118	a chiral nematic mesomorphic structure	81:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	0	66	theme	nematic	90:96	arg1	structure					110:118	a chiral nematic mesomorphic structure	81:118	a chiral nematic mesomorphic structure	81:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	1	67	theme	monomer	354:360	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	67	theme	monomer	354:360	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	4	68	theme	composition	911:921	arg1	Polymorphism					758:769	Polymorphism	758:769	Polymorphism of CaCO3 deposited inside the films	758:805	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	4	68	theme	composition	911:921	arg1	function					849:856	a function	847:856	a function of the soaking time (1-5 day) and also of the matrix composition	847:921	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	0	69	from	mineralization	6:19	arg1	composites					32:41	polymer composites	24:41	polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure	24:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	8	70	theme	helical	1614:1620	arg1	pitch					1622:1626	the helical pitch	1610:1626	the helical pitch	1610:1626	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	2	71	theme	2-hydroxyethyl	504:517	arg1	acid					543:546	2-hydroxyethyl methacrylate-co-acrylic acid	504:546	2-hydroxyethyl methacrylate-co-acrylic acid	504:546	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	2	71	theme	2-hydroxyethyl	504:517	arg1	CNC/poly					495:502	CNC/poly	495:502	CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol)	495:592	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	6	72	theme	calcite	1228:1234	arg1	prominent					1240:1248	prominent	1240:1248	prominent	1240:1248	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	6	72	theme	calcite	1228:1234	arg1	formation					1208:1216	the formation	1204:1216	the formation of stable calcite	1204:1234	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	1	73	theme	%	381:381	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	73	theme	%	381:381	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	74	from	CNC	383:385	arg1	feed					390:393	feed	390:393	feed	390:393	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	5	75	theme	1-day	1018:1022	arg1	soaking					1024:1030	1-day soaking	1018:1030	1-day soaking	1018:1030	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	75	theme	1-day	1018:1022	arg1	carbonate					1001:1009	amorphous calcium carbonate	983:1009	amorphous calcium carbonate (ACC) (1-day soaking)	983:1031	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	0	76	theme	polymer	24:30	arg1	composites					32:41	polymer composites	24:41	polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure	24:118	CaCO3 mineralization in polymer composites with cellulose nanocrystals providing a chiral nematic mesomorphic structure.
31499114	2	77	theme	polymer	411:417	arg1	composites					419:428	polymer composites	411:428	polymer composites	411:428	Two series of polymer composites were prepared: CNC/poly(2-hydroxyethyl methacrylate) (PHEMA) and CNC/poly(2-hydroxyethyl methacrylate-co-acrylic acid) (P(HEMA-co-AA), HEMA:AA = 95:5-70:30 in mol).
31499114	7	78	theme	film	1493:1496	arg1	matrix					1498:1503	the swollen film matrix	1481:1503	the swollen film matrix	1481:1503	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	9	79	theme	heat/flame	1903:1912	arg1	performance					1890:1900	the thermal performance	1878:1900	the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites	1878:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	9	79	theme	heat/flame	1903:1912	arg1	resistance					1914:1923	heat/flame resistance	1903:1923	heat/flame resistance	1903:1923	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	5	80	theme	5-day	1133:1137	arg1	vaterite					1109:1116	vaterite	1109:1116	vaterite	1109:1116	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	80	theme	5-day	1133:1137	arg1	soaking					1139:1145	5-day soaking	1133:1145	5-day soaking	1133:1145	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	81	theme	3-day	1069:1073	arg1	soaking					1075:1081	3-day soaking	1069:1081	3-day soaking	1069:1081	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	5	81	theme	3-day	1069:1073	arg1	vaterite					1059:1066	metastable crystalline vaterite	1036:1066	metastable crystalline vaterite (3-day soaking)	1036:1082	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	7	82	theme	anionic	1381:1387	arg1	group					1389:1393	an anionic group	1378:1393	an anionic group (-SO3- or -COO-)	1378:1410	It was deduced that the mesofiller CNC and the AA unit in the vinyl polymer, both bearing an anionic group (-SO3- or -COO-), contributed to capturing Ca2+ to facilitate the CaCO3 deposition in the swollen film matrix.
31499114	5	83	theme	polymorphic	953:963	arg1	form					965:968	the polymorphic form	949:968	the polymorphic form	949:968	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	4	84	theme	deposited	780:788	arg1	Polymorphism					758:769	Polymorphism	758:769	Polymorphism of CaCO3 deposited inside the films	758:805	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	4	84	theme	deposited	780:788	arg1	function					849:856	a function	847:856	a function of the soaking time (1-5 day) and also of the matrix composition	847:921	Polymorphism of CaCO3 deposited inside the films was examined by X-ray diffractometry as a function of the soaking time (1-5 day) and also of the matrix composition.
31499114	1	85	theme	aqueous	336:342	arg1	lyotropics					362:371	the precursory aqueous CNC/vinyl monomer lyotropics	321:371	the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed)	321:394	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	1	85	theme	aqueous	336:342	arg1	CNC					383:385	7-11 wt% CNC	374:385	7-11 wt% CNC	374:385	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
31499114	6	86	from	mineralization	1156:1169	arg1	prominent					1240:1248	prominent	1240:1248	prominent	1240:1248	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	6	86	from	mineralization	1156:1169	arg1	formation					1208:1216	the formation	1204:1216	the formation of stable calcite	1204:1234	In the mineralization of the CNC/P(HEMA-co-AA) series, the formation of stable calcite was prominent besides minor appearance of vaterite.
31499114	5	87	theme	CNC/PHEMA	931:939	arg1	series					941:946	the CNC/PHEMA series	927:946	the CNC/PHEMA series	927:946	In the CNC/PHEMA series, the polymorphic form changed from amorphous calcium carbonate (ACC) (1-day soaking) to metastable crystalline vaterite (3-day soaking) and then to a mixture of vaterite and aragonite (5-day soaking).
31499114	9	88	theme	order-retaining	1945:1959	arg1	composites					1973:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	the mesomorphic order-retaining CNC/polymer composites	1929:1982	Thermogravimetry showed that the mineralization definitely improved the thermal performance (heat/flame resistance) of the mesomorphic order-retaining CNC/polymer composites.
31499114	8	89	theme	pre-invested	1510:1521	arg1	organization					1538:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization	1506:1549	The pre-invested chiral nematic organization was kept in any of the mineralized films (dried); however, the helical pitch was appreciably reduced relative to that observed before the mineralization, attributable to the increase of ionic strength in the CNCs' surroundings accompanied by the wet process.
31499114	1	90	theme	assembly	253:260	arg1	structure					236:244	a chiral nematic structure	219:244	a chiral nematic structure of CNC assembly	219:260	CaCO3 mineralization was carried out using cellulose nanocrystal (CNC)/polymer composites wherein a chiral nematic structure of CNC assembly was immobilized in advance via a polymerization process of the precursory aqueous CNC/vinyl monomer lyotropics (7-11 wt% CNC in feed).
30579901	5	0	theme	highest	1053:1059	arg1	weight					1071:1076	highest molecular weight	1053:1076	highest molecular weight of ~55% and 16 × 104 g·mol-1	1053:1105	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	1	theme	barley	467:472	arg1	bran					474:477	barley bran	467:477	barley bran	467:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	2	2	used	used	586:589	arg2	types					563:567	two types	559:567	two types of xylanase	559:579	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	2	used	used	586:589	arg2	enzymes					490:496	Different enzymes	480:496	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase	480:579	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	2	used	used	586:589	arg2	glucoamylase					529:540	glucoamylase	529:540	glucoamylase	529:540	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	2	used	used	586:589	arg2	α-amylase					508:516	α-amylase	508:516	α-amylase	508:516	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	2	used	used	586:589	arg2	pullulanase					543:553	pullulanase	543:553	pullulanase	543:553	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	2	used	used	586:589	arg2	protease					519:526	protease	519:526	protease	519:526	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	4	3	theme	%	968:968	arg1	weight					955:960	lowest molecular weight	938:960	lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively	938:1002	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	4	3	theme	%	968:968	arg1	purity					927:932	highest β-glucan purity	910:932	highest β-glucan purity	910:932	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	1	4	theme	weight	255:260	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	4	theme	weight	255:260	arg1	behavior					288:295	rheological behavior	276:295	rheological behavior	276:295	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	4	theme	weight	255:260	arg1	properties					314:323	microstructural properties	298:323	microstructural properties	298:323	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	4	theme	weight	255:260	arg1	factors					340:346	compositional factors	326:346	compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran	326:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	0	5	theme	rheological	76:86	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	5	theme	rheological	76:86	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	5	6	theme	molecular	1061:1069	arg1	weight					1071:1076	highest molecular weight	1053:1076	highest molecular weight of ~55% and 16 × 104 g·mol-1	1053:1105	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	7	theme	industrial	1435:1444	arg1	processes					1446:1454	the digestive track and industrial processes	1411:1454	processes	1446:1454	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	8	theme	study	160:164	arg1	aim					148:150	The aim	144:150	The aim of this study	144:164	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	5	9	theme	extraction	1191:1200	arg1	yield					1202:1206	extraction yield	1191:1206	extraction yield	1191:1206	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	10	theme	α-amylase	1129:1137	arg1	treatment					1139:1147	only α-amylase treatment	1124:1147	only α-amylase treatment	1124:1147	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	11	theme	respective	1015:1024	arg1	purity					1042:1047	β-glucan purity	1033:1047	β-glucan purity	1033:1047	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	0	12	from	Effect	0:5	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	12	from	Effect	0:5	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	5	13	dep	purity	1042:1047	arg1	the					1011:1013	the	1011:1013	the	1011:1013	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	4	14	theme	2 × 104 g·mol-1	974:988	arg1	weight					955:960	lowest molecular weight	938:960	lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively	938:1002	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	4	14	theme	2 × 104 g·mol-1	974:988	arg1	purity					927:932	highest β-glucan purity	910:932	highest β-glucan purity	910:932	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	0	15	theme	microstructural	92:106	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	15	theme	microstructural	92:106	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	3	16	theme	three-step	657:666	arg1	procedures					681:690	The three-step purification procedures	653:690	The three-step purification procedures with α-amylase, protease and xylanase	653:728	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	16	theme	three-step	657:666	arg1	increasing					766:775	increasing	766:775	increasing the β-glucan content	766:796	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	16	theme	three-step	657:666	arg1	removing					805:812	removing	805:812	removing starch, protein and pentosans from the barley bran in 4 h	805:870	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	16	theme	three-step	657:666	arg1	efficient					753:761	efficient	753:761	efficient	753:761	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	4	17	dep	β-glucan	918:925	arg1	highest					910:916	highest	910:916	highest	910:916	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	5	18	dep	showed	1251:1256	arg1	affect					1289:1294	affect	1289:1294	affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes	1289:1454	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	19	theme	rheological	276:286	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	19	theme	rheological	276:286	arg1	behavior					288:295	rheological behavior	276:295	rheological behavior	276:295	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	0	20	theme	barley	122:127	arg1	β-glucan					134:141	barley bran β-glucan	122:141	barley bran β-glucan	122:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	3	21	theme	purification	668:679	arg1	procedures					681:690	The three-step purification procedures	653:690	The three-step purification procedures with α-amylase, protease and xylanase	653:728	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	21	theme	purification	668:679	arg1	increasing					766:775	increasing	766:775	increasing the β-glucan content	766:796	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	21	theme	purification	668:679	arg1	removing					805:812	removing	805:812	removing starch, protein and pentosans from the barley bran in 4 h	805:870	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	21	theme	purification	668:679	arg1	efficient					753:761	efficient	753:761	efficient	753:761	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	2	22	theme	Different	480:488	arg1	pullulanase					543:553	pullulanase	543:553	pullulanase	543:553	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	22	theme	Different	480:488	arg1	α-amylase					508:516	α-amylase	508:516	α-amylase	508:516	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	22	theme	Different	480:488	arg1	protease					519:526	protease	519:526	protease	519:526	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	22	theme	Different	480:488	arg1	types					563:567	two types	559:567	two types of xylanase	559:579	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	22	theme	Different	480:488	arg1	enzymes					490:496	Different enzymes	480:496	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase	480:579	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	22	theme	Different	480:488	arg1	glucoamylase					529:540	glucoamylase	529:540	glucoamylase	529:540	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	23	theme	xylanase	572:579	arg1	α-amylase					508:516	α-amylase	508:516	α-amylase	508:516	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	23	theme	xylanase	572:579	arg1	pullulanase					543:553	pullulanase	543:553	pullulanase	543:553	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	23	theme	xylanase	572:579	arg1	types					563:567	two types	559:567	two types of xylanase	559:579	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	23	theme	xylanase	572:579	arg1	glucoamylase					529:540	glucoamylase	529:540	glucoamylase	529:540	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	2	23	theme	xylanase	572:579	arg1	protease					519:526	protease	519:526	protease	519:526	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	5	24	theme	β-glucan	1033:1040	arg1	purity					1042:1047	β-glucan purity	1033:1047	β-glucan purity	1033:1047	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	25	theme	extraction	411:420	arg1	yield					422:426	extraction yield	411:426	extraction yield (%) of the fiber samples obtained from barley bran	411:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	25	theme	extraction	411:420	arg1	%					429:429	%	429:429	%	429:429	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	5	26	theme	digestive	1415:1423	arg1	track					1425:1429	the digestive track and industrial processes	1411:1454	track	1425:1429	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	27	theme	enzymatic	1267:1275	arg1	extractions					1277:1287	enzymatic extractions	1267:1287	enzymatic extractions	1267:1287	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	28	theme	lowest	1026:1031	arg1	purity					1042:1047	β-glucan purity	1033:1047	β-glucan purity	1033:1047	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	2	29	from	bran	647:650	arg1	β-glucan					626:633	extract β-glucan	618:633	extract β-glucan from barley bran	618:650	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	4	30	theme	molecular	945:953	arg1	weight					955:960	lowest molecular weight	938:960	lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively	938:1002	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	5	31	theme	16 × 104 g·mol-1	1090:1105	arg1	purity					1042:1047	β-glucan purity	1033:1047	β-glucan purity	1033:1047	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	31	theme	16 × 104 g·mol-1	1090:1105	arg1	weight					1071:1076	highest molecular weight	1053:1076	highest molecular weight of ~55% and 16 × 104 g·mol-1	1053:1105	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	0	32	theme	enzymatic	20:28	arg1	extractions					30:40	different enzymatic extractions	10:40	different enzymatic extractions	10:40	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	33	theme	β-glucan	134:141	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	33	theme	β-glucan	134:141	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	2	34	theme	special	594:600	arg1	combinations					602:613	special combinations	594:613	special combinations to extract β-glucan from barley bran	594:650	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	5	35	theme	β-glucan-enriched	1338:1354	arg1	fibers					1356:1361	the β-glucan-enriched fibers	1334:1361	the β-glucan-enriched fibers	1334:1361	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	0	36	theme	different	10:18	arg1	extractions					30:40	different enzymatic extractions	10:40	different enzymatic extractions	10:40	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	1	37	theme	microstructural	298:312	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	37	theme	microstructural	298:312	arg1	properties					314:323	microstructural properties	298:323	microstructural properties	298:323	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	0	38	theme	bran	129:132	arg1	β-glucan					134:141	barley bran β-glucan	122:141	barley bran β-glucan	122:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	3	39	theme	barley	853:858	arg1	bran					860:863	the barley bran	849:863	the barley bran in 4 h	849:870	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	5	40	theme	study	1245:1249	arg1	results					1229:1235	the results	1225:1235	the results of this study	1225:1249	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	41	theme	different	200:208	arg1	procedures					231:240	different enzymatic extraction procedures	200:240	different enzymatic extraction procedures	200:240	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	0	42	theme	extractions	30:40	arg1	Effect					0:5	Effect	0:5	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.	0:142	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	4	43	theme	β-glucan	918:925	arg1	purity					927:932	highest β-glucan purity	910:932	highest β-glucan purity	910:932	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	3	44	from	bran	860:863	arg1	4 h					868:870	4 h	868:870	4 h	868:870	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	1	45	theme	enzymatic	210:218	arg1	procedures					231:240	different enzymatic extraction procedures	200:240	different enzymatic extraction procedures	200:240	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	0	46	theme	weight	55:60	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	46	theme	weight	55:60	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	1	47	theme	compositional	326:338	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	47	theme	compositional	326:338	arg1	factors					340:346	compositional factors	326:346	compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran	326:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	2	48	theme	barley	640:645	arg1	bran					647:650	barley bran	640:650	barley bran	640:650	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	0	49	theme	molecular	45:53	arg1	properties					108:117	rheological and microstructural properties	76:117	rheological and microstructural properties of barley bran β-glucan	76:141	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	0	49	theme	molecular	45:53	arg1	distribution					62:73	molecular weight distribution	45:73	molecular weight distribution	45:73	Effect of different enzymatic extractions on molecular weight distribution, rheological and microstructural properties of barley bran β-glucan.
30579901	5	50	theme	%	1084:1084	arg1	purity					1042:1047	β-glucan purity	1033:1047	β-glucan purity	1033:1047	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	50	theme	%	1084:1084	arg1	weight					1071:1076	highest molecular weight	1053:1076	highest molecular weight of ~55% and 16 × 104 g·mol-1	1053:1105	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	3	51	dep	efficient	753:761	arg1	procedures					681:690	The three-step purification procedures	653:690	The three-step purification procedures with α-amylase, protease and xylanase	653:728	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	51	dep	efficient	753:761	arg1	increasing					766:775	increasing	766:775	increasing the β-glucan content	766:796	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	51	dep	efficient	753:761	arg1	efficient					753:761	efficient	753:761	efficient	753:761	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	51	dep	efficient	753:761	arg1	removing					805:812	removing	805:812	removing starch, protein and pentosans from the barley bran in 4 h	805:870	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	52	with	procedures	681:690	arg1	α-amylase					697:705	α-amylase	697:705	α-amylase	697:705	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	52	with	procedures	681:690	arg1	xylanase					721:728	xylanase	721:728	xylanase	721:728	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	3	52	with	procedures	681:690	arg1	protease					708:715	protease	708:715	protease	708:715	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	1	53	from	effects	189:195	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	53	from	effects	189:195	arg1	behavior					288:295	rheological behavior	276:295	rheological behavior	276:295	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	53	from	effects	189:195	arg1	properties					314:323	microstructural properties	298:323	microstructural properties	298:323	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	53	from	effects	189:195	arg1	factors					340:346	compositional factors	326:346	compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran	326:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	2	54	theme	extract	618:624	arg1	β-glucan					626:633	extract β-glucan	618:633	extract β-glucan from barley bran	618:650	Different enzymes including α-amylase, protease, glucoamylase, pullulanase and two types of xylanase were used in special combinations to extract β-glucan from barley bran.
30579901	1	55	theme	fiber	439:443	arg1	samples					445:451	the fiber samples	435:451	the fiber samples obtained from barley bran	435:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	56	theme	extraction	220:229	arg1	procedures					231:240	different enzymatic extraction procedures	200:240	different enzymatic extraction procedures	200:240	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	5	57	theme	fibers	1356:1361	arg1	relationship					1318:1329	molecular-rheological relationship	1296:1329	molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes	1296:1454	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	58	theme	samples	445:451	arg1	pentosans					379:387	pentosans	379:387	pentosans (%)	379:391	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	%					404:404	%	404:404	%	404:404	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	starch					367:372	starch	367:372	starch (%)	367:376	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	%					390:390	%	390:390	%	390:390	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	%					375:375	%	375:375	%	375:375	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	ash					358:360	ash	358:360	ash (%)	358:364	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	β-glucan					394:401	β-glucan	394:401	β-glucan (%)	394:405	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	%					363:363	%	363:363	%	363:363	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	yield					422:426	extraction yield	411:426	extraction yield (%) of the fiber samples obtained from barley bran	411:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	58	theme	samples	445:451	arg1	%					429:429	%	429:429	%	429:429	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	3	59	theme	β-glucan	781:788	arg1	content					790:796	the β-glucan content	777:796	the β-glucan content	777:796	The three-step purification procedures with α-amylase, protease and xylanase have been proved to be efficient in increasing the β-glucan content and in removing starch, protein and pentosans from the barley bran in 4 h.
30579901	1	60	theme	procedures	231:240	arg1	effects					189:195	the effects	185:195	the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran	185:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	4	61	theme	lowest	938:943	arg1	weight					955:960	lowest molecular weight	938:960	lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively	938:1002	The procedures also have resulted in highest β-glucan purity and lowest molecular weight of ~89% and 2 × 104 g·mol-1, respectively.
30579901	5	62	theme	different	1173:1181	arg1	purity					1183:1188	different purity	1173:1188	different purity	1173:1188	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	63	theme	molecular-rheological	1296:1316	arg1	relationship					1318:1329	molecular-rheological relationship	1296:1329	molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes	1296:1454	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	64	from	function	1399:1406	arg1	track					1425:1429	the digestive track and industrial processes	1411:1454	track	1425:1429	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	5	64	from	function	1399:1406	arg1	processes					1446:1454	the digestive track and industrial processes	1411:1454	processes	1446:1454	While the respective lowest β-glucan purity and highest molecular weight of ~55% and 16 × 104 g·mol-1 were obtained by only α-amylase treatment in 1.5 h. Regardless of different purity, extraction yield and composition, the results of this study showed that how enzymatic extractions affect molecular-rheological relationship of the β-glucan-enriched fibers, which may help to understand their function in the digestive track and industrial processes.
30579901	1	65	theme	molecular	245:253	arg1	distribution					262:273	molecular weight distribution	245:273	molecular weight distribution	245:273	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	65	theme	molecular	245:253	arg1	behavior					288:295	rheological behavior	276:295	rheological behavior	276:295	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	65	theme	molecular	245:253	arg1	properties					314:323	microstructural properties	298:323	microstructural properties	298:323	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
30579901	1	65	theme	molecular	245:253	arg1	factors					340:346	compositional factors	326:346	compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran	326:477	The aim of this study was to investigate the effects of different enzymatic extraction procedures on molecular weight distribution, rheological behavior, microstructural properties, compositional factors including ash (%), starch (%), pentosans (%), β-glucan (%) and extraction yield (%) of the fiber samples obtained from barley bran.
31525416	3	0	theme	EPS	688:690	arg1	P-EPS					693:697	the B-2 pure EPS (P-EPS)	675:698	the B-2 pure EPS (P-EPS)	675:698	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	5	1	theme	B-2	830:832	arg1	EPS					834:836	B-2 EPS	830:836	B-2 EPS	830:836	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	9	2	theme	skimmed	1311:1317	arg1	milk					1319:1322	sucrose-supplemented skimmed milk	1290:1322	sucrose-supplemented skimmed milk	1290:1322	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	5	3	theme	certain	842:848	arg1	DPPH					881:884	DPPH	881:884	DPPH	881:884	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	3	theme	certain	842:848	arg1	1,1-diphenyl-2-picrylhydrazyl					850:878	certain 1,1-diphenyl-2-picrylhydrazyl	842:878	certain 1,1-diphenyl-2-picrylhydrazyl (DPPH)	842:885	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	9	4	theme	different	1363:1371	arg1	degrees					1373:1379	different degrees	1363:1379	different degrees of solidification	1363:1397	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	1	5	theme	response	276:283	arg1	RSM					306:308	RSM	306:308	RSM	306:308	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	1	5	theme	response	276:283	arg1	methodology					293:303	response surface methodology	276:303	response surface methodology (RSM)	276:309	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	3	6	theme	B-2	679:681	arg1	P-EPS					693:697	the B-2 pure EPS (P-EPS)	675:698	the B-2 pure EPS (P-EPS)	675:698	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	6	7	theme	B-2	1053:1055	arg1	EPS					1057:1059	the B-2 EPS	1049:1059	the B-2 EPS	1049:1059	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	1	8	theme	surface	285:291	arg1	RSM					306:308	RSM	306:308	RSM	306:308	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	1	8	theme	surface	285:291	arg1	methodology					293:303	response surface methodology	276:303	response surface methodology (RSM)	276:309	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	10	9	theme	bacteria	1451:1458	arg1	growth					1431:1436	the growth	1427:1436	the growth of probiotic bacteria, especially Lactobacillus delbrueckii	1427:1496	Moreover, B-2 EPS promoted the growth of probiotic bacteria, especially Lactobacillus delbrueckii.
31525416	9	10	theme	solidification	1384:1397	arg1	degrees					1373:1379	different degrees	1363:1379	different degrees of solidification	1363:1397	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	9	11	theme	milk	1319:1322	arg1	concentrations					1272:1285	different concentrations	1262:1285	different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification	1262:1397	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	10	12	theme	probiotic	1441:1449	arg1	delbrueckii					1486:1496	Lactobacillus delbrueckii	1472:1496	Lactobacillus delbrueckii	1472:1496	Moreover, B-2 EPS promoted the growth of probiotic bacteria, especially Lactobacillus delbrueckii.
31525416	10	12	theme	probiotic	1441:1449	arg1	bacteria					1451:1458	probiotic bacteria	1441:1458	probiotic bacteria	1441:1458	Moreover, B-2 EPS promoted the growth of probiotic bacteria, especially Lactobacillus delbrueckii.
31525416	5	13	theme	scavenging	977:986	arg1	abilities					988:996	nitroso radical (NO2-) scavenging abilities	954:996	nitroso radical (NO2-) scavenging abilities	954:996	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	1	14	with	conditions	146:155	arg1	structure					209:217	a highly branched structure	191:217	a highly branched structure extracted from Leuconostoc citreum B-2	191:256	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	6	15	theme	EPS	1057:1059	arg1	temperature					1034:1044	The degradation temperature	1018:1044	The degradation temperature of the B-2 EPS	1018:1059	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	6	15	theme	EPS	1057:1059	arg1	313 °C					1065:1070	313 °C	1065:1070	313 °C	1065:1070	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	2	16	theme	pH	408:409	arg1	conditions					367:376	the optimal fermentation conditions	342:376	the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	342:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	9	17	theme	sucrose-supplemented	1290:1309	arg1	milk					1319:1322	sucrose-supplemented skimmed milk	1290:1322	sucrose-supplemented skimmed milk	1290:1322	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	5	18	theme	reducing	1002:1009	arg1	power					1011:1015	reducing power	1002:1015	reducing power	1002:1015	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	7	19	from	effect	1145:1150	arg1	oils					1165:1168	vegetable oils	1155:1168	vegetable oils	1155:1168	The emulsification effect on vegetable oils was better than that of hydrocarbons.
31525416	0	20	dep	Leuconostoc	104:114	arg1	citreum					116:122	Leuconostoc citreum B-2	104:126	Leuconostoc citreum B-2	104:126	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	0	20	dep	Leuconostoc	104:114	arg1	B-2					124:126	B-2	124:126	Leuconostoc citreum B-2	104:126	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	3	21	theme	P-EPS	693:697	arg1	sulfur					656:661	sulfur	656:661	sulfur	656:661	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	3	21	theme	P-EPS	693:697	arg1	nitrogen					643:650	nitrogen	643:650	nitrogen	643:650	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	3	21	theme	P-EPS	693:697	arg1	%					717:717	39.05 ± 0.25%	705:717	39.05 ± 0.25%	705:717	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	3	21	theme	P-EPS	693:697	arg1	hydrogen					633:640	hydrogen	633:640	hydrogen	633:640	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	3	21	theme	P-EPS	693:697	arg1	carbon					625:630	the carbon	621:630	the carbon	621:630	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	2	22	theme	extract	396:402	arg1	conditions					367:376	the optimal fermentation conditions	342:376	the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	342:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	4	23	theme	acid	791:794	arg1	high					800:803	high	800:803	high	800:803	The content of uronic acid was high, reaching 20.94 ± 6.30%.
31525416	4	23	theme	acid	791:794	arg1	content					773:779	The content	769:779	The content of uronic acid	769:794	The content of uronic acid was high, reaching 20.94 ± 6.30%.
31525416	4	24	theme	uronic	784:789	arg1	acid					791:794	uronic acid	784:794	uronic acid	784:794	The content of uronic acid was high, reaching 20.94 ± 6.30%.
31525416	1	25	theme	branched	200:207	arg1	structure					209:217	a highly branched structure	191:217	a highly branched structure extracted from Leuconostoc citreum B-2	191:256	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	2	26	theme	6.12	438:441	arg1	pH					408:409	pH	408:409	pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	408:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	26	theme	6.12	438:441	arg1	sucrose					381:387	sucrose	381:387	sucrose	381:387	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	26	theme	6.12	438:441	arg1	extract					396:402	yeast extract	390:402	yeast extract	390:402	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	6	27	contain	have	1097:1100	arg2	stability					1115:1123	high thermal stability	1102:1123	high thermal stability	1102:1123	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	6	27	contain	have	1097:1100	arg1	EPS					1093:1095	B-2 EPS	1089:1095	B-2 EPS	1089:1095	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	6	28	theme	thermal	1107:1113	arg1	stability					1115:1123	high thermal stability	1102:1123	high thermal stability	1102:1123	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	2	29	theme	5.94 g/L	425:432	arg1	pH					408:409	pH	408:409	pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	408:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	29	theme	5.94 g/L	425:432	arg1	sucrose					381:387	sucrose	381:387	sucrose	381:387	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	29	theme	5.94 g/L	425:432	arg1	extract					396:402	yeast extract	390:402	yeast extract	390:402	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	5	30	theme	nitroso	954:960	arg1	radical					962:968	nitroso radical	954:968	nitroso radical (NO2-) scavenging abilities	954:996	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	30	theme	nitroso	954:960	arg1	NO2-					971:974	NO2-	971:974	NO2-	971:974	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	6	31	theme	degradation	1022:1032	arg1	temperature					1034:1044	The degradation temperature	1018:1044	The degradation temperature of the B-2 EPS	1018:1059	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	6	31	theme	degradation	1022:1032	arg1	313 °C					1065:1070	313 °C	1065:1070	313 °C	1065:1070	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	2	32	theme	EPS	466:468	arg1	59.33 ± 1.34 g/L					480:495	59.33 ± 1.34 g/L	480:495	59.33 ± 1.34 g/L	480:495	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	32	theme	EPS	466:468	arg1	yield					470:474	the B-2 EPS yield	458:474	the B-2 EPS yield	458:474	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	33	theme	optimal	346:352	arg1	conditions					367:376	the optimal fermentation conditions	342:376	the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	342:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	5	34	theme	radical	962:968	arg1	abilities					988:996	nitroso radical (NO2-) scavenging abilities	954:996	nitroso radical (NO2-) scavenging abilities	954:996	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	3	35	theme	composition	588:598	arg1	analysis					600:607	Chemical composition analysis	579:607	Chemical composition analysis	579:607	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	2	36	theme	yeast	390:394	arg1	extract					396:402	yeast extract	390:402	yeast extract	390:402	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	37	theme	99.80 g/L	414:422	arg1	pH					408:409	pH	408:409	pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	408:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	37	theme	99.80 g/L	414:422	arg1	sucrose					381:387	sucrose	381:387	sucrose	381:387	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	37	theme	99.80 g/L	414:422	arg1	extract					396:402	yeast extract	390:402	yeast extract	390:402	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	38	theme	B-2	462:464	arg1	59.33 ± 1.34 g/L					480:495	59.33 ± 1.34 g/L	480:495	59.33 ± 1.34 g/L	480:495	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	38	theme	B-2	462:464	arg1	yield					470:474	the B-2 EPS yield	458:474	the B-2 EPS yield	458:474	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	0	39	theme	exopolysaccharides	37:54	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	0	39	theme	exopolysaccharides	37:54	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	6	40	theme	high	1102:1105	arg1	stability					1115:1123	high thermal stability	1102:1123	high thermal stability	1102:1123	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	3	41	dep	carbon	625:630	arg1	contents					663:670	contents	663:670	contents	663:670	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	9	42	theme	citreum	1233:1239	arg1	B-2					1241:1243	citreum B-2	1233:1243	citreum B-2	1233:1243	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	10	43	theme	B-2	1410:1412	arg1	EPS					1414:1416	B-2 EPS	1410:1416	B-2 EPS	1410:1416	Moreover, B-2 EPS promoted the growth of probiotic bacteria, especially Lactobacillus delbrueckii.
31525416	5	44	theme	hydrogen	928:935	arg1	H2O2					947:950	H2O2	947:950	H2O2	947:950	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	44	theme	hydrogen	928:935	arg1	peroxide					937:944	hydrogen peroxide	928:944	hydrogen peroxide (H2O2)	928:951	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	0	45	with	characterization	17:32	arg1	structure					79:87	a highly branched structure	61:87	a highly branched structure	61:87	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	2	46	theme	fermentation	354:365	arg1	conditions					367:376	the optimal fermentation conditions	342:376	the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	342:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	47	theme	greater	519:525	arg1	times					513:517	5.93 times	508:517	5.93 times greater than that from the initial nonoptimized conditions	508:576	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	2	48	theme	nonoptimized	554:565	arg1	conditions					567:576	the initial nonoptimized conditions	542:576	the initial nonoptimized conditions	542:576	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	3	49	theme	Chemical	579:586	arg1	analysis					600:607	Chemical composition analysis	579:607	Chemical composition analysis	579:607	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	7	50	theme	vegetable	1155:1163	arg1	oils					1165:1168	vegetable oils	1155:1168	vegetable oils	1155:1168	The emulsification effect on vegetable oils was better than that of hydrocarbons.
31525416	1	51	theme	fermentation	133:144	arg1	conditions					146:155	The fermentation conditions	129:155	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2	129:256	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	2	52	theme	initial	546:552	arg1	conditions					567:576	the initial nonoptimized conditions	542:576	the initial nonoptimized conditions	542:576	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31525416	6	53	theme	B-2	1089:1091	arg1	EPS					1093:1095	B-2 EPS	1089:1095	B-2 EPS	1089:1095	The degradation temperature of the B-2 EPS was 313 °C, indicating that B-2 EPS have high thermal stability.
31525416	5	54	theme	superoxide	904:913	arg1	O2-					922:924	O2-	922:924	O2-	922:924	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	54	theme	superoxide	904:913	arg1	anion					915:919	superoxide anion	904:919	superoxide anion (O2-)	904:925	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	0	55	with	Optimization	0:11	arg1	structure					79:87	a highly branched structure	61:87	a highly branched structure	61:87	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	5	56	contain	had	838:840	arg2	peroxide					937:944	hydrogen peroxide	928:944	hydrogen peroxide (H2O2)	928:951	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	abilities					988:996	nitroso radical (NO2-) scavenging abilities	954:996	nitroso radical (NO2-) scavenging abilities	954:996	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	DPPH					881:884	DPPH	881:884	DPPH	881:884	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	power					1011:1015	reducing power	1002:1015	reducing power	1002:1015	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	hydroxyl					888:895	hydroxyl	888:895	hydroxyl (·OH)	888:901	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	anion					915:919	superoxide anion	904:919	superoxide anion (O2-)	904:925	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	·OH					898:900	·OH	898:900	·OH	898:900	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	1,1-diphenyl-2-picrylhydrazyl					850:878	certain 1,1-diphenyl-2-picrylhydrazyl	842:878	certain 1,1-diphenyl-2-picrylhydrazyl (DPPH)	842:885	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	O2-					922:924	O2-	922:924	O2-	922:924	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg2	H2O2					947:950	H2O2	947:950	H2O2	947:950	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	5	56	contain	had	838:840	arg1	EPS					834:836	B-2 EPS	830:836	B-2 EPS	830:836	B-2 EPS had certain 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (·OH), superoxide anion (O2-), hydrogen peroxide (H2O2), nitroso radical (NO2-) scavenging abilities and reducing power.
31525416	9	57	contain	have	1358:1361	arg1	milk					1350:1353	the skimmed milk	1338:1353	the skimmed milk	1338:1353	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	9	57	contain	have	1358:1361	arg2	degrees					1373:1379	different degrees	1363:1379	different degrees of solidification	1363:1397	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	1	58	dep	Leuconostoc	234:244	arg1	citreum					246:252	Leuconostoc citreum B-2	234:256	Leuconostoc citreum B-2	234:256	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	1	58	dep	Leuconostoc	234:244	arg1	B-2					254:256	B-2	254:256	Leuconostoc citreum B-2	234:256	The fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM).
31525416	7	59	theme	emulsification	1130:1143	arg1	better					1174:1179	better	1174:1179	better	1174:1179	The emulsification effect on vegetable oils was better than that of hydrocarbons.
31525416	7	59	theme	emulsification	1130:1143	arg1	effect					1145:1150	The emulsification effect	1126:1150	The emulsification effect on vegetable oils	1126:1168	The emulsification effect on vegetable oils was better than that of hydrocarbons.
31525416	9	60	theme	skimmed	1342:1348	arg1	milk					1350:1353	the skimmed milk	1338:1353	the skimmed milk	1338:1353	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	9	61	theme	different	1262:1270	arg1	concentrations					1272:1285	different concentrations	1262:1285	different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification	1262:1397	citreum B-2 was activated in different concentrations of sucrose-supplemented skimmed milk, which caused the skimmed milk to have different degrees of solidification.
31525416	0	62	theme	branched	70:77	arg1	structure					79:87	a highly branched structure	61:87	a highly branched structure	61:87	Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2.
31525416	3	63	theme	pure	683:686	arg1	P-EPS					693:697	the B-2 pure EPS (P-EPS)	675:698	the B-2 pure EPS (P-EPS)	675:698	Chemical composition analysis showed that the carbon, hydrogen, nitrogen and sulfur contents of the B-2 pure EPS (P-EPS) were 39.05 ± 0.25%, 7.19 ± 0.08%, 0.72 ± 0.03% and 0%, respectively.
31525416	2	64	theme	sucrose	381:387	arg1	conditions					367:376	the optimal fermentation conditions	342:376	the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively	342:455	The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.94 g/L and 6.12, respectively, the B-2 EPS yield was 59.33 ± 1.34 g/L, which was 5.93 times greater than that from the initial nonoptimized conditions.
31122447	3	0	theme	classical	346:354	arg1	method					356:361	The classical method	342:361	The classical method	342:361	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	5	1	from	crosslinker	820:830	arg1	media					880:884	an aqueous media	869:884	an aqueous media	869:884	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	10	2	theme	oligosaccharides	1568:1583	arg1	isolation					1555:1563	isolation	1555:1563	isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1555:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	7	3	theme	binding	1085:1091	arg1	capacity					1093:1100	binding capacity	1085:1100	binding capacity	1085:1100	This binding to the targeted molecule was affected by reaction temperature with regard to binding capacity and specificity.
31122447	4	4	theme	polymer	619:625	arg1	material					627:634	a novel polymer material	611:634	a novel polymer material	611:634	In this study, we explored the possibility for rapid separation of oligosaccharides using a novel polymer material.
31122447	0	5	theme	imprinted	89:97	arg1	polymer					99:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	Selective binding of heparin oligosaccharides in a magnetic thermoresponsive molecularly imprinted polymer.
31122447	6	6	theme	specific	952:959	arg1	binding					961:967	specific binding	952:967	specific binding to the template molecule	952:992	Incubation of the MIP with hepairn oligosaccharides demonstrated specific binding to the template molecule.
31122447	9	7	theme	pseudo-second-order	1242:1260	arg1	model					1270:1274	The pseudo-second-order kinetic model	1238:1274	The pseudo-second-order kinetic model	1238:1274	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	4	8	theme	novel	613:617	arg1	material					627:634	a novel polymer material	611:634	a novel polymer material	611:634	In this study, we explored the possibility for rapid separation of oligosaccharides using a novel polymer material.
31122447	8	9	theme	monomers	1228:1235	arg1	compositions					1195:1206	the compositions	1191:1206	the compositions of multi-functional monomers	1191:1235	The recognition specificity and selectivity can be modulated by varying the compositions of multi-functional monomers.
31122447	9	10	theme	kinetic	1262:1268	arg1	model					1270:1274	The pseudo-second-order kinetic model	1238:1274	The pseudo-second-order kinetic model	1238:1274	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	5	11	theme	magnetic	641:648	arg1	MIPs					699:702	MIPs	699:702	MIPs	699:702	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	5	11	theme	magnetic	641:648	arg1	polymers					689:696	The magnetic thermoresponsive molecularly imprinted polymers	637:696	The magnetic thermoresponsive molecularly imprinted polymers (MIPs)	637:703	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	10	12	from	polysaccharides	1596:1610	arg1	isolation					1555:1563	isolation	1555:1563	isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1555:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	12	from	polysaccharides	1596:1610	arg1	sulfate					1626:1632	heparan sulfate	1618:1632	heparan sulfate	1618:1632	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	12	from	polysaccharides	1596:1610	arg1	sulfate					1651:1657	chondoriting sulfate	1638:1657	chondoriting sulfate	1638:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	12	from	polysaccharides	1596:1610	arg1	oligosaccharides					1568:1583	oligosaccharides	1568:1583	oligosaccharides from other polysaccharides	1568:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	8	13	theme	multi-functional	1211:1226	arg1	monomers					1228:1235	multi-functional monomers	1211:1235	multi-functional monomers	1211:1235	The recognition specificity and selectivity can be modulated by varying the compositions of multi-functional monomers.
31122447	5	14	theme	thermoresponsive	650:665	arg1	MIPs					699:702	MIPs	699:702	MIPs	699:702	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	5	14	theme	thermoresponsive	650:665	arg1	polymers					689:696	The magnetic thermoresponsive molecularly imprinted polymers	637:696	The magnetic thermoresponsive molecularly imprinted polymers (MIPs)	637:703	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	2	15	theme	disaccharides	300:312	arg1	compositions					284:295	its molecular structure e.g. compositions	255:295	its molecular structure e.g. compositions of disaccharides and oligosaccharides units	255:339	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	10	16	theme	heparin	1496:1502	arg1	tetra-saccharides					1475:1491	tetra-saccharides	1475:1491	tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1475:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	16	theme	heparin	1496:1502	arg1	di-					1467:1469	di-	1467:1469	di-	1467:1469	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	17	theme	chondoriting	1638:1649	arg1	sulfate					1651:1657	chondoriting sulfate	1638:1657	chondoriting sulfate	1638:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	17	theme	chondoriting	1638:1649	arg1	oligosaccharides					1568:1583	oligosaccharides	1568:1583	oligosaccharides from other polysaccharides	1568:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	6	18	theme	hepairn	914:920	arg1	oligosaccharides					922:937	hepairn oligosaccharides	914:937	hepairn oligosaccharides	914:937	Incubation of the MIP with hepairn oligosaccharides demonstrated specific binding to the template molecule.
31122447	5	19	theme	imprinted	679:687	arg1	MIPs					699:702	MIPs	699:702	MIPs	699:702	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	5	19	theme	imprinted	679:687	arg1	polymers					689:696	The magnetic thermoresponsive molecularly imprinted polymers	637:696	The magnetic thermoresponsive molecularly imprinted polymers (MIPs)	637:703	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	10	20	theme	rapid	1447:1451	arg1	separation					1453:1462	rapid separation	1447:1462	rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1447:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	21	theme	heparan	1618:1624	arg1	sulfate					1626:1632	heparan sulfate	1618:1632	heparan sulfate	1618:1632	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	21	theme	heparan	1618:1624	arg1	oligosaccharides					1568:1583	oligosaccharides	1568:1583	oligosaccharides from other polysaccharides	1568:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	1	22	theme	thrombotic	188:197	arg1	diseases					199:206	thrombotic diseases	188:206	thrombotic diseases	188:206	Heparin is a highly sulfated polysaccharide, applied in clinic for treatment of thrombotic diseases.
31122447	6	23	with	Incubation	887:896	arg1	oligosaccharides					922:937	hepairn oligosaccharides	914:937	hepairn oligosaccharides	914:937	Incubation of the MIP with hepairn oligosaccharides demonstrated specific binding to the template molecule.
31122447	9	24	theme	Langmuir	1280:1287	arg1	model					1298:1302	Langmuir isotherm model	1280:1302	Langmuir isotherm model	1280:1302	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	3	25	theme	nitrous	436:442	arg1	s					451:451	nitrous acid I s	436:451	nitrous acid I s	436:451	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	6	26	theme	MIP	905:907	arg1	Incubation					887:896	Incubation	887:896	Incubation of the MIP with hepairn oligosaccharides	887:937	Incubation of the MIP with hepairn oligosaccharides demonstrated specific binding to the template molecule.
31122447	9	27	theme	isotherm	1289:1296	arg1	model					1298:1302	Langmuir isotherm model	1280:1302	Langmuir isotherm model	1280:1302	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	3	28	theme	size	456:459	arg1	chromatography					471:484	size exclusion chromatography	456:484	size exclusion chromatography	456:484	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	0	29	theme	Selective	0:8	arg1	binding					10:16	Selective binding	0:16	Selective binding of heparin	0:27	Selective binding of heparin oligosaccharides in a magnetic thermoresponsive molecularly imprinted polymer.
31122447	4	30	theme	oligosaccharides	588:603	arg1	separation					574:583	rapid separation	568:583	rapid separation of oligosaccharides	568:603	In this study, we explored the possibility for rapid separation of oligosaccharides using a novel polymer material.
31122447	10	31	theme	tetra-saccharides	1475:1491	arg1	separation					1453:1462	rapid separation	1447:1462	rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1447:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	3	32	theme	exclusion	461:469	arg1	chromatography					471:484	size exclusion chromatography	456:484	size exclusion chromatography	456:484	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	0	33	theme	heparin	21:27	arg1	binding					10:16	Selective binding	0:16	Selective binding of heparin	0:27	Selective binding of heparin oligosaccharides in a magnetic thermoresponsive molecularly imprinted polymer.
31122447	5	34	theme	surface	835:841	arg1	polymerization					851:864	surface radical polymerization	835:864	surface radical polymerization	835:864	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	2	35	theme	units	335:339	arg1	compositions					284:295	its molecular structure e.g. compositions	255:295	its molecular structure e.g. compositions of disaccharides and oligosaccharides units	255:339	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	10	36	theme	di-	1467:1469	arg1	separation					1453:1462	rapid separation	1447:1462	rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate	1447:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	3	37	theme	time-consuming	497:510	arg1	oligosaccharides					378:393	the oligosaccharides	374:393	the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process	374:518	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	3	37	theme	time-consuming	497:510	arg1	process					512:518	a time-consuming process	495:518	a time-consuming process	495:518	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	8	38	theme	recognition	1123:1133	arg1	specificity					1135:1145	The recognition specificity	1119:1145	The recognition specificity	1119:1145	The recognition specificity and selectivity can be modulated by varying the compositions of multi-functional monomers.
31122447	2	39	theme	oligosaccharides	318:333	arg1	units					335:339	oligosaccharides units	318:339	oligosaccharides units	318:339	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	9	40	theme	best	1316:1319	arg1	fit					1321:1323	the best fit	1312:1323	the best fit to the equilibrium adsorption of heparin disaccharides by MIPs	1312:1386	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	1	41	theme	diseases	199:206	arg1	treatment					175:183	treatment	175:183	treatment of thrombotic diseases	175:206	Heparin is a highly sulfated polysaccharide, applied in clinic for treatment of thrombotic diseases.
31122447	5	42	theme	aqueous	872:878	arg1	media					880:884	an aqueous media	869:884	an aqueous media	869:884	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	5	43	theme	radical	843:849	arg1	polymerization					851:864	surface radical polymerization	835:864	surface radical polymerization	835:864	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	3	44	theme	acid	444:447	arg1	s					451:451	nitrous acid I s	436:451	nitrous acid I s	436:451	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	5	45	theme	heparin	728:734	arg1	disaccharide					736:747	heparin disaccharide	728:747	heparin disaccharide	728:747	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	5	45	theme	heparin	728:734	arg1	template					754:761	a template	752:761	a template	752:761	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	0	46	theme	thermoresponsive	60:75	arg1	polymer					99:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	Selective binding of heparin oligosaccharides in a magnetic thermoresponsive molecularly imprinted polymer.
31122447	10	47	theme	new	1418:1420	arg1	material					1422:1429	the new material	1414:1429	the new material	1414:1429	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	48	dep	oligosaccharides	1568:1583	arg1	sulfate					1626:1632	heparan sulfate	1618:1632	heparan sulfate	1618:1632	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	48	dep	oligosaccharides	1568:1583	arg1	sulfate					1651:1657	chondoriting sulfate	1638:1657	chondoriting sulfate	1638:1657	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	10	48	dep	oligosaccharides	1568:1583	arg1	oligosaccharides					1568:1583	oligosaccharides	1568:1583	oligosaccharides from other polysaccharides	1568:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	3	49	theme	I	449:449	arg1	s					451:451	nitrous acid I s	436:451	nitrous acid I s	436:451	The classical method to isolate the oligosaccharides after depolymerization by heparinases or nitrous acid I s by size exclusion chromatography which is a time-consuming process.
31122447	6	50	theme	template	976:983	arg1	molecule					985:992	the template molecule	972:992	the template molecule	972:992	Incubation of the MIP with hepairn oligosaccharides demonstrated specific binding to the template molecule.
31122447	10	51	theme	other	1590:1594	arg1	polysaccharides					1596:1610	other polysaccharides	1590:1610	other polysaccharides	1590:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	9	52	theme	equilibrium	1332:1342	arg1	adsorption					1344:1353	the equilibrium adsorption	1328:1353	the equilibrium adsorption of heparin disaccharides by MIPs	1328:1386	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	9	53	theme	disaccharides	1366:1378	arg1	adsorption					1344:1353	the equilibrium adsorption	1328:1353	the equilibrium adsorption of heparin disaccharides by MIPs	1328:1386	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	1	54	theme	sulfated	128:135	arg1	polysaccharide					137:150	a highly sulfated polysaccharide	119:150	a highly sulfated polysaccharide	119:150	Heparin is a highly sulfated polysaccharide, applied in clinic for treatment of thrombotic diseases.
31122447	1	54	theme	sulfated	128:135	arg1	Heparin					108:114	Heparin	108:114	Heparin	108:114	Heparin is a highly sulfated polysaccharide, applied in clinic for treatment of thrombotic diseases.
31122447	4	55	theme	rapid	568:572	arg1	separation					574:583	rapid separation	568:583	rapid separation of oligosaccharides	568:603	In this study, we explored the possibility for rapid separation of oligosaccharides using a novel polymer material.
31122447	2	56	theme	biological	213:222	arg1	related					244:250	related	244:250	related	244:250	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	2	56	theme	biological	213:222	arg1	activity					224:231	The biological activity	209:231	The biological activity	209:231	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	5	57	theme	functional	788:797	arg1	monomer					799:805	functional monomer	788:805	functional monomer	788:805	The magnetic thermoresponsive molecularly imprinted polymers (MIPs) were synthesized using heparin disaccharide as a template, AEM, NIPAAm, and AAm as functional monomer, and MBAA as crosslinker by surface radical polymerization in an aqueous media.
31122447	7	58	theme	reaction	1049:1056	arg1	temperature					1058:1068	reaction temperature	1049:1068	reaction temperature	1049:1068	This binding to the targeted molecule was affected by reaction temperature with regard to binding capacity and specificity.
31122447	2	59	theme	structure	269:277	arg1	compositions					284:295	its molecular structure e.g. compositions	255:295	its molecular structure e.g. compositions of disaccharides and oligosaccharides units	255:339	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	10	60	used	used	1438:1441	arg2	material					1422:1429	the new material	1414:1429	the new material	1414:1429	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31122447	2	61	theme	molecular	259:267	arg1	structure					269:277	its molecular structure	255:277	its molecular structure e.g. compositions of disaccharides and oligosaccharides units	255:339	The biological activity is closely related to its molecular structure e.g. compositions of disaccharides and oligosaccharides units.
31122447	0	62	theme	magnetic	51:58	arg1	polymer					99:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	a magnetic thermoresponsive molecularly imprinted polymer	49:105	Selective binding of heparin oligosaccharides in a magnetic thermoresponsive molecularly imprinted polymer.
31122447	9	63	theme	heparin	1358:1364	arg1	disaccharides					1366:1378	heparin disaccharides	1358:1378	heparin disaccharides	1358:1378	The pseudo-second-order kinetic model and Langmuir isotherm model provide the best fit to the equilibrium adsorption of heparin disaccharides by MIPs.
31122447	7	64	theme	targeted	1015:1022	arg1	molecule					1024:1031	the targeted molecule	1011:1031	the targeted molecule	1011:1031	This binding to the targeted molecule was affected by reaction temperature with regard to binding capacity and specificity.
31122447	10	65	from	isolation	1555:1563	arg1	polysaccharides					1596:1610	other polysaccharides	1590:1610	other polysaccharides	1590:1610	The results suggest that the new material can be used for rapid separation of di- and tetra-saccharides of heparin, which can also be adapted to the applications for isolation of oligosaccharides from other polysaccharides, e.g. heparan sulfate and chondoriting sulfate.
31253317	7	0	theme	lactose	1065:1071	arg1	crystallization					1073:1087	lactose crystallization	1065:1087	lactose crystallization	1065:1087	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	1	1	from	difference	92:101	arg1	formula					113:119	infant formula	106:119	infant formula (IF)	106:124	Compositional difference in infant formula (IF) tends to influence its functionality and storage behaviour.
31253317	4	2	theme	Scanning	493:500	arg1	microscopy					511:520	Scanning electron microscopy	493:520	Scanning electron microscopy	493:520	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	7	3	theme	crystallization	1073:1087	arg1	role					1057:1060	a possible role	1046:1060	a possible role of lactose crystallization in preferential migration of triglycerides to particle surface	1046:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	2	4	dep	commercial	282:291	arg1	A					297:297	A	297:297	A	297:297	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	2	4	dep	commercial	282:291	arg1	B					303:303	B	303:303	B	303:303	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	7	5	from	%	1022:1022	arg1	others					1027:1032	others	1027:1032	others	1027:1032	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	3	6	theme	X-ray	343:347	arg1	diffraction					349:359	X-ray diffraction	343:359	X-ray diffraction	343:359	Lactose crystallization measured by X-ray diffraction ranged between 2 and 32 % and was observed to decrease with increasing IF stages, which directly correlates with their composition.
31253317	7	7	theme	restricted	879:888	arg1	presence					890:897	restricted presence	879:897	restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF	879:971	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	8	theme	fatty	937:941	arg1	acids					943:947	unsaturated fatty acids	925:947	unsaturated fatty acids (C18:1 and C18:2)	925:965	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	8	theme	fatty	937:941	arg1	C18:2					960:964	C18:2	960:964	C18:2	960:964	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	8	theme	fatty	937:941	arg1	C18:1					950:954	C18:1	950:954	C18:1	950:954	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	2	9	theme	commercial	282:291	arg1	stages					268:273	different stages	258:273	different stages of two commercial IF (A and B)	258:304	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	7	10	theme	free-fat	854:861	arg1	analysis					863:870	Surface free-fat analysis	846:870	Surface free-fat analysis	846:870	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	11	theme	acids	943:947	arg1	presence					890:897	restricted presence	879:897	restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF	879:971	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	1	12	theme	Compositional	78:90	arg1	difference					92:101	Compositional difference	78:101	Compositional difference in infant formula (IF)	78:124	Compositional difference in infant formula (IF) tends to influence its functionality and storage behaviour.
31253317	1	13	theme	storage	167:173	arg1	behaviour					175:183	storage behaviour	167:183	storage behaviour	167:183	Compositional difference in infant formula (IF) tends to influence its functionality and storage behaviour.
31253317	7	14	theme	preferential	1092:1103	arg1	migration					1105:1113	preferential migration	1092:1113	preferential migration of triglycerides to particle surface	1092:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	15	theme	possible	1048:1055	arg1	role					1057:1060	a possible role	1046:1060	a possible role of lactose crystallization in preferential migration of triglycerides to particle surface	1046:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	16	theme	particle	1135:1142	arg1	surface					1144:1150	particle surface	1135:1150	particle surface	1135:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	17	theme	unsaturated	925:935	arg1	acids					943:947	unsaturated fatty acids	925:947	unsaturated fatty acids (C18:1 and C18:2)	925:965	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	17	theme	unsaturated	925:935	arg1	C18:2					960:964	C18:2	960:964	C18:2	960:964	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	17	theme	unsaturated	925:935	arg1	C18:1					950:954	C18:1	950:954	C18:1	950:954	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	18	theme	Surface	846:852	arg1	analysis					863:870	Surface free-fat analysis	846:870	Surface free-fat analysis	846:870	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	0	19	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and surface composition of infant formula powders.
31253317	6	20	theme	fat	774:776	arg1	composition					778:788	surface-free fat composition	761:788	surface-free fat composition	761:788	Bulk and surface-free fat composition was significantly (p < 0.05) different for all samples.
31253317	1	21	theme	infant	106:111	arg1	formula					113:119	infant formula	106:119	infant formula (IF)	106:124	Compositional difference in infant formula (IF) tends to influence its functionality and storage behaviour.
31253317	5	22	theme	lactose	712:718	arg1	crystallization					720:734	lactose crystallization	712:734	lactose crystallization	712:734	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	0	23	theme	surface	31:37	arg1	composition					39:49	surface composition	31:49	surface composition	31:49	Physicochemical properties and surface composition of infant formula powders.
31253317	7	24	theme	fat	917:919	arg1	%					904:904	5-10%	900:904	5-10% of surface fat	900:919	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	24	theme	fat	917:919	arg1	fat					917:919	surface fat	909:919	surface fat	909:919	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	6	25	theme	surface-free	761:772	arg1	composition					778:788	surface-free fat composition	761:788	surface-free fat composition	761:788	Bulk and surface-free fat composition was significantly (p < 0.05) different for all samples.
31253317	6	26	dep	significantly	794:806	arg1	p < 0.05					809:816	p < 0.05	809:816	p < 0.05	809:816	Bulk and surface-free fat composition was significantly (p < 0.05) different for all samples.
31253317	2	27	dep	composition	211:221	arg1	the					207:209	the	207:209	the	207:209	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	4	28	theme	lactose	560:566	arg1	presence					536:543	the presence	532:543	the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size	532:632	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	5	29	theme	surface	696:702	arg1	fat					704:706	surface fat	696:706	surface fat	696:706	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	5	30	located	observed	679:686	arg1	contrary					642:649	the contrary	638:649	the contrary	638:649	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	5	30	located	observed	679:686	arg2	correlation					663:673	a negative correlation	652:673	a negative correlation	652:673	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	5	30	located	observed	679:686	arg1	samples					743:749	all samples	739:749	all samples	739:749	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	4	31	theme	particle	620:627	arg1	size					629:632	the powder particle size	609:632	the powder particle size	609:632	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	4	32	theme	crystalline	548:558	arg1	lactose					560:566	crystalline lactose	548:566	crystalline lactose which significantly (p < 0.05) increased the powder particle size	548:632	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	2	33	theme	physico-chemical	227:242	arg1	properties					244:253	physico-chemical properties	227:253	physico-chemical properties	227:253	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	7	34	from	role	1057:1060	arg1	migration					1105:1113	preferential migration	1092:1113	preferential migration of triglycerides to particle surface	1092:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	4	35	dep	significantly	574:586	arg1	p < 0.05					589:596	p < 0.05	589:596	p < 0.05	589:596	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	7	36	theme	triglycerides	1118:1130	arg1	migration					1105:1113	preferential migration	1092:1113	preferential migration of triglycerides to particle surface	1092:1150	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	37	theme	higher	978:983	arg1	lactose					997:1003	higher crystalline lactose	978:1003	higher crystalline lactose	978:1003	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	0	38	theme	formula	61:67	arg1	powders					69:75	infant formula powders	54:75	infant formula powders	54:75	Physicochemical properties and surface composition of infant formula powders.
31253317	4	39	theme	electron	502:509	arg1	microscopy					511:520	Scanning electron microscopy	493:520	Scanning electron microscopy	493:520	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	5	40	theme	negative	654:661	arg1	correlation					663:673	a negative correlation	652:673	a negative correlation	652:673	On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples.
31253317	7	41	theme	crystalline	985:995	arg1	lactose					997:1003	higher crystalline lactose	978:1003	higher crystalline lactose	978:1003	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	2	42	dep	A	297:297	arg1	IF					293:294	IF	293:294	IF	293:294	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	0	43	theme	infant	54:59	arg1	powders					69:75	infant formula powders	54:75	infant formula powders	54:75	Physicochemical properties and surface composition of infant formula powders.
31253317	2	44	theme	stages	268:273	arg1	composition					211:221	composition	211:221	composition	211:221	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	2	44	theme	stages	268:273	arg1	properties					244:253	physico-chemical properties	227:253	physico-chemical properties	227:253	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	7	45	theme	surface	909:915	arg1	fat					917:919	surface fat	909:919	surface fat	909:919	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	2	46	theme	different	258:266	arg1	stages					268:273	different stages	258:273	different stages of two commercial IF (A and B)	258:304	The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B).
31253317	7	47	dep	presence	890:897	arg1	%					904:904	5-10%	900:904	5-10% of surface fat	900:919	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	7	47	dep	presence	890:897	arg1	fat					917:919	surface fat	909:919	surface fat	909:919	Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
31253317	3	48	theme	Lactose	307:313	arg1	crystallization					315:329	Lactose crystallization	307:329	Lactose crystallization measured by X-ray diffraction	307:359	Lactose crystallization measured by X-ray diffraction ranged between 2 and 32 % and was observed to decrease with increasing IF stages, which directly correlates with their composition.
31253317	4	49	theme	powder	613:618	arg1	size					629:632	the powder particle size	609:632	the powder particle size	609:632	Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size.
31253317	0	50	theme	powders	69:75	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and surface composition of infant formula powders.
31253317	0	50	theme	powders	69:75	arg1	composition					39:49	surface composition	31:49	surface composition	31:49	Physicochemical properties and surface composition of infant formula powders.
31513223	0	0	theme	pseudomallei	111:122	arg1	epitopes					86:93	the predominant epitopes	70:93	the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens	70:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	2	1	theme	measures	528:535	arg1	development					500:510	the development	496:510	the development of prophylactic measures against melioidosis and glanders	496:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	6	2	theme	synthetic	1156:1164	arg1	compounds					1166:1174	these synthetic compounds	1150:1174	these synthetic compounds	1150:1174	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	2	3	theme	prophylactic	515:526	arg1	measures					528:535	prophylactic measures	515:535	prophylactic measures against melioidosis and glanders	515:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	1	4	theme	potential	371:379	arg1	glanders					176:183	glanders	176:183	glanders	176:183	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	4	theme	potential	371:379	arg1	Melioidosis					160:170	Melioidosis	160:170	Melioidosis	160:170	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	4	theme	potential	371:379	arg1	agents					394:399	potential bioterrorism agents	371:399	potential bioterrorism agents	371:399	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	4	5	theme	Bp	924:925	arg1	epitopes					912:919	the main substitution epitopes	890:919	the main substitution epitopes of Bp	890:925	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	4	6	theme	Bm	931:932	arg1	OAgs					938:941	Bm LPS OAgs	931:941	Bm LPS OAgs	931:941	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	1	7	theme	bioterrorism	381:392	arg1	glanders					176:183	glanders	176:183	glanders	176:183	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	7	theme	bioterrorism	381:392	arg1	Melioidosis					160:170	Melioidosis	160:170	Melioidosis	160:170	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	7	theme	bioterrorism	381:392	arg1	agents					394:399	potential bioterrorism agents	371:399	potential bioterrorism agents	371:399	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	4	8	theme	LPS	934:936	arg1	OAgs					938:941	Bm LPS OAgs	931:941	Bm LPS OAgs	931:941	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	5	9	theme	inner	1089:1093	arg1	rhamnose					1095:1102	the inner rhamnose	1085:1102	the inner rhamnose into a 6-deoxy-l-talose residue	1085:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	5	10	theme	glycosylation	1013:1025	arg1	strategy					1027:1034	a sequential glycosylation strategy	1000:1034	a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue	1000:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	6	11	theme	melioidosis	1219:1229	arg1	serum					1239:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	1	12	from	issues	336:341	arg1	countries					357:365	developing countries	346:365	developing countries	346:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	7	13	theme	suitable	1355:1362	arg1	candidates					1364:1373	suitable candidates	1355:1373	suitable candidates for the development of vaccines and/or diagnostic tools against melioidosis and glanders	1355:1462	Our results suggest that these tetrasaccharides could be suitable candidates for the development of vaccines and/or diagnostic tools against melioidosis and glanders.
31513223	4	14	theme	substitution	899:910	arg1	epitopes					912:919	the main substitution epitopes	890:919	the main substitution epitopes of Bp	890:925	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	7	15	theme	vaccines	1398:1405	arg1	development					1383:1393	the development	1379:1393	the development of vaccines and/or diagnostic tools against melioidosis and glanders	1379:1462	Our results suggest that these tetrasaccharides could be suitable candidates for the development of vaccines and/or diagnostic tools against melioidosis and glanders.
31513223	7	16	theme	tools	1425:1429	arg1	development					1383:1393	the development	1379:1393	the development of vaccines and/or diagnostic tools against melioidosis and glanders	1379:1462	Our results suggest that these tetrasaccharides could be suitable candidates for the development of vaccines and/or diagnostic tools against melioidosis and glanders.
31513223	0	17	theme	mallei	141:146	arg1	epitopes					86:93	the predominant epitopes	70:93	the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens	70:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	4	18	theme	main	894:897	arg1	epitopes					912:919	the main substitution epitopes	890:919	the main substitution epitopes of Bp	890:925	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	1	19	theme	Gram-negative	213:225	arg1	bacteria					227:234	the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm)	209:294	the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm)	209:294	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	0	20	dep	pseudomallei	111:122	arg1	O-antigens					148:157	O-antigens	148:157	O-antigens	148:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	3	21	theme	d-glucose	730:738	arg1	residues					761:768	d-glucose and 6-deoxy-l-talose residues	730:768	d-glucose and 6-deoxy-l-talose residues	730:768	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	2	22	theme	vaccine	473:479	arg1	candidates					481:490	attractive vaccine candidates	462:490	attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders	462:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	22	theme	vaccine	473:479	arg1	lipopolysaccharides					412:430	Bp and Bm lipopolysaccharides	402:430	Bp and Bm lipopolysaccharides (LPS)	402:436	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	23	theme	Bm	409:410	arg1	candidates					481:490	attractive vaccine candidates	462:490	attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders	462:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	23	theme	Bm	409:410	arg1	LPS					433:435	LPS	433:435	LPS	433:435	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	23	theme	Bm	409:410	arg1	lipopolysaccharides					412:430	Bp and Bm lipopolysaccharides	402:430	Bp and Bm lipopolysaccharides (LPS)	402:436	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	1	24	from	agents	394:399	arg1	countries					357:365	developing countries	346:365	developing countries	346:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	6	25	theme	native	1283:1288	arg1	OAg					1293:1295	native Bp OAg	1283:1295	native Bp OAg	1283:1295	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	3	26	theme	similar	602:608	arg1	LPSs					610:613	structurally similar LPSs	589:613	structurally similar LPSs	589:613	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	3	27	theme	OAg	638:640	arg1	portion					643:649	the O-antigen (OAg) portion	623:649	the O-antigen (OAg) portion	623:649	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	0	28	theme	serum-reactive	20:33	arg1	tetrasaccharides					45:60	serum-reactive synthetic tetrasaccharides	20:60	serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens	20:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	1	29	theme	urgent	315:320	arg1	issues					336:341	urgent public health issues	315:341	urgent public health issues in developing countries	315:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	29	theme	urgent	315:320	arg1	glanders					176:183	glanders	176:183	glanders	176:183	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	29	theme	urgent	315:320	arg1	Melioidosis					160:170	Melioidosis	160:170	Melioidosis	160:170	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	3	30	theme	repeating	685:693	arg1	unit					695:698	unit	695:698	unit	695:698	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	3	30	theme	repeating	685:693	arg1	disaccharide					705:716	a disaccharide	703:716	a disaccharide composed of d-glucose and 6-deoxy-l-talose residues	703:768	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	5	31	theme	sequential	1002:1011	arg1	strategy					1027:1034	a sequential glycosylation strategy	1000:1034	a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue	1000:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	3	32	dep	consists	651:658	arg1	acetylated					798:807	acetylated	798:807	acetylated	798:807	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	3	32	dep	consists	651:658	arg1	methylated					813:822	methylated	813:822	methylated	813:822	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	1	33	theme	public	322:327	arg1	issues					336:341	urgent public health issues	315:341	urgent public health issues in developing countries	315:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	33	theme	public	322:327	arg1	glanders					176:183	glanders	176:183	glanders	176:183	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	33	theme	public	322:327	arg1	Melioidosis					160:170	Melioidosis	160:170	Melioidosis	160:170	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	34	dep	bacteria	227:234	arg1	mallei					284:289	Burkholderia mallei	271:289	Burkholderia mallei (Bm)	271:294	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	34	dep	bacteria	227:234	arg1	Bp					263:264	Bp	263:264	Bp	263:264	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	34	dep	bacteria	227:234	arg1	Bm					292:293	Bm	292:293	Bm	292:293	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	34	dep	bacteria	227:234	arg1	pseudomallei					249:260	Burkholderia pseudomallei	236:260	Burkholderia pseudomallei (Bp)	236:265	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	7	35	theme	diagnostic	1414:1423	arg1	tools					1425:1429	diagnostic tools	1414:1429	diagnostic tools	1414:1429	Our results suggest that these tetrasaccharides could be suitable candidates for the development of vaccines and/or diagnostic tools against melioidosis and glanders.
31513223	1	36	theme	health	329:334	arg1	issues					336:341	urgent public health issues	315:341	urgent public health issues in developing countries	315:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	36	theme	health	329:334	arg1	glanders					176:183	glanders	176:183	glanders	176:183	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	1	36	theme	health	329:334	arg1	Melioidosis					160:170	Melioidosis	160:170	Melioidosis	160:170	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	0	37	theme	synthetic	35:43	arg1	tetrasaccharides					45:60	serum-reactive synthetic tetrasaccharides	20:60	serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens	20:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	4	38	theme	tetrasaccharides	863:878	arg1	synthesis					846:854	the synthesis	842:854	the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs	842:941	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	0	39	theme	predominant	74:84	arg1	epitopes					86:93	the predominant epitopes	70:93	the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens	70:157	Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes of Burkholderia pseudomallei and Burkholderia mallei O-antigens.
31513223	3	40	theme	O-antigen	627:635	arg1	portion					643:649	the O-antigen (OAg) portion	623:649	the O-antigen (OAg) portion	623:649	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	6	41	theme	Thai	1214:1217	arg1	serum					1239:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	2	42	theme	attractive	462:471	arg1	candidates					481:490	attractive vaccine candidates	462:490	attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders	462:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	42	theme	attractive	462:471	arg1	lipopolysaccharides					412:430	Bp and Bm lipopolysaccharides	402:430	Bp and Bm lipopolysaccharides (LPS)	402:436	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	4	43	theme	OAgs	938:941	arg1	synthesis					846:854	the synthesis	842:854	the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs	842:941	Herein we report the synthesis of two tetrasaccharides mimicking the main substitution epitopes of Bp and Bm LPS OAgs.
31513223	5	44	theme	rhamnose	1095:1102	arg1	epimerization					1068:1080	the late-stage epimerization	1053:1080	the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue	1053:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	6	45	theme	culture-confirmed	1196:1212	arg1	serum					1239:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	1	46	theme	developing	346:355	arg1	countries					357:365	developing countries	346:365	developing countries	346:365	Melioidosis and glanders, respectively caused by the Gram-negative bacteria Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), are considered as urgent public health issues in developing countries and potential bioterrorism agents.
31513223	6	47	theme	OAg	1293:1295	arg1	antigenicity					1267:1278	the antigenicity	1263:1278	the antigenicity of native Bp OAg	1263:1295	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	3	48	dep	express	581:587	arg1	wherein					615:621	wherein	615:621	wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated	615:822	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	6	49	theme	patient	1231:1237	arg1	serum					1239:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	culture-confirmed Thai melioidosis patient serum	1196:1243	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	5	50	theme	6-deoxy-l-talose	1111:1126	arg1	residue					1128:1134	a 6-deoxy-l-talose residue	1109:1134	a 6-deoxy-l-talose residue	1109:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	6	51	theme	Bp	1290:1291	arg1	OAg					1293:1295	native Bp OAg	1283:1295	native Bp OAg	1283:1295	We show that these synthetic compounds strongly react with culture-confirmed Thai melioidosis patient serum and closely mimic the antigenicity of native Bp OAg.
31513223	5	52	dep	strategy	1027:1034	arg1	relying					1042:1048	relying	1042:1048	relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue	1042:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	5	53	theme	tetrasaccharides	964:979	arg1	assembly					948:955	The assembly	944:955	The assembly of the tetrasaccharides	944:979	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	5	54	theme	late-stage	1057:1066	arg1	epimerization					1068:1080	the late-stage epimerization	1053:1080	the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue	1053:1134	The assembly of the tetrasaccharides was achieved using a sequential glycosylation strategy while relying on the late-stage epimerization of the inner rhamnose into a 6-deoxy-l-talose residue.
31513223	3	55	theme	6-deoxy-l-talose	744:759	arg1	residues					761:768	d-glucose and 6-deoxy-l-talose residues	730:768	d-glucose and 6-deoxy-l-talose residues	730:768	Bp and Bm express structurally similar LPSs wherein the O-antigen (OAg) portion consists of a heteropolymer whose repeating unit is a disaccharide composed of d-glucose and 6-deoxy-l-talose residues, the latter being diversely acetylated and methylated.
31513223	2	56	theme	Bp	402:403	arg1	candidates					481:490	attractive vaccine candidates	462:490	attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders	462:568	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	56	theme	Bp	402:403	arg1	LPS					433:435	LPS	433:435	LPS	433:435	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
31513223	2	56	theme	Bp	402:403	arg1	lipopolysaccharides					412:430	Bp and Bm lipopolysaccharides	402:430	Bp and Bm lipopolysaccharides (LPS)	402:436	Bp and Bm lipopolysaccharides (LPS) have been identified as attractive vaccine candidates for the development of prophylactic measures against melioidosis and glanders.
29763701	0	0	theme	bodies	105:110	arg1	polysaccharides					44:58	water soluble non-digestible polysaccharides	15:58	water soluble non-digestible polysaccharides	15:58	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	0	0	theme	bodies	105:110	arg1	activities					82:91	anti-inflammatory activities	64:91	anti-inflammatory activities of fruiting bodies	64:110	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	5	1	theme	helix	686:690	arg1	conformation					692:703	triple helix conformation	679:703	triple helix conformation	679:703	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	7	2	theme	COX-2	1045:1049	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	2	theme	COX-2	1045:1049	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	3	3	theme	strains	562:568	arg1	bodies					525:530	fruiting bodies	516:530	fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	516:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	0	4	theme	fruiting	96:103	arg1	bodies					105:110	fruiting bodies	96:110	fruiting bodies	96:110	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	7	5	theme	TXNP	1132:1135	arg1	potency					1121:1127	the potency	1117:1127	the potency of TXNP	1117:1135	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	5	theme	TXNP	1132:1135	arg1	stronger					1141:1148	stronger	1141:1148	stronger	1141:1148	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	1	6	theme	important	198:206	arg1	β-glucans					184:192	β-glucans	184:192	β-glucans	184:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	6	theme	important	198:206	arg1	Polysaccharides					158:172	Polysaccharides	158:172	Polysaccharides including β-glucans	158:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	6	theme	important	198:206	arg1	components					218:227	important bioactive components	198:227	important bioactive components of mushroom	198:239	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	0	7	from	Differences	0:10	arg1	polysaccharides					44:58	water soluble non-digestible polysaccharides	15:58	water soluble non-digestible polysaccharides	15:58	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	0	7	from	Differences	0:10	arg1	activities					82:91	anti-inflammatory activities	64:91	anti-inflammatory activities of fruiting bodies	64:110	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	0	7	from	Differences	0:10	arg1	strains					149:155	two cultivated Xylaria nigripes strains	117:155	two cultivated Xylaria nigripes strains	117:155	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	7	8	theme	PGE2	1004:1007	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	8	theme	PGE2	1004:1007	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	5	9	contain	had	673:675	arg2	weight					719:724	molecular weight	709:724	molecular weight	709:724	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	9	contain	had	673:675	arg1	TXNP					668:671	TXNP	668:671	TXNP	668:671	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	9	contain	had	673:675	arg2	conformation					692:703	triple helix conformation	679:703	triple helix conformation	679:703	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	10	theme	CXNP	772:775	arg1	14.7 kDa					781:788	14.7 kDa	781:788	14.7 kDa	781:788	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	10	theme	CXNP	772:775	arg1	weight					762:767	the molecular weight	748:767	the molecular weight of CXNP	748:775	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	11	theme	molecular	709:717	arg1	weight					719:724	molecular weight	709:724	molecular weight	709:724	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	8	12	theme	anti-inflammatory	1373:1389	arg1	potency					1391:1397	their anti-inflammatory potency	1367:1397	their anti-inflammatory potency	1367:1397	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	3	13	from	activities	436:445	arg1	bodies					525:530	fruiting bodies	516:530	fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	516:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	7	14	theme	TNF-α	994:998	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	14	theme	TNF-α	994:998	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	1	15	theme	bioactive	208:216	arg1	β-glucans					184:192	β-glucans	184:192	β-glucans	184:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	15	theme	bioactive	208:216	arg1	Polysaccharides					158:172	Polysaccharides	158:172	Polysaccharides including β-glucans	158:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	15	theme	bioactive	208:216	arg1	components					218:227	important bioactive components	198:227	important bioactive components of mushroom	198:239	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	0	16	theme	cultivated	121:130	arg1	strains					149:155	two cultivated Xylaria nigripes strains	117:155	two cultivated Xylaria nigripes strains	117:155	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	6	17	theme	monosaccharide	795:808	arg1	glucose					848:854	glucose	848:854	glucose	848:854	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	6	17	theme	monosaccharide	795:808	arg1	composition					810:820	The monosaccharide composition	791:820	The monosaccharide composition of TXNP	791:828	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	8	18	theme	physicochemical	1256:1270	arg1	characteristics					1272:1286	the different physicochemical characteristics	1242:1286	the different physicochemical characteristics of polysaccharides from TXN and CXN	1242:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	8	19	theme	cultivation	1203:1213	arg1	conditions					1189:1198	similar conditions	1181:1198	similar conditions of cultivation and extraction procedures	1181:1239	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	3	20	theme	anti-inflammatory	418:434	arg1	activities					436:445	anti-inflammatory activities	418:445	anti-inflammatory activities	418:445	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	21	dep	polysaccharides	479:493	arg1	CXNP					505:508	CXNP	505:508	CXNP	505:508	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	21	dep	polysaccharides	479:493	arg1	TXNP					496:499	TXNP	496:499	TXNP	496:499	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	21	dep	polysaccharides	479:493	arg1	polysaccharides					479:493	water soluble non-digestible polysaccharides	450:493	water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	450:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	7	22	theme	IL-1β	987:991	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	22	theme	IL-1β	987:991	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	3	23	dep	strains	562:568	arg1	CXN					579:581	CXN	579:581	CXN	579:581	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	23	dep	strains	562:568	arg1	TXN					571:573	TXN	571:573	TXN	571:573	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	23	dep	strains	562:568	arg1	strains					562:568	two cultivated X. nigripes strains	535:568	two cultivated X. nigripes strains (TXN and CXN)	535:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	2	24	used	used	303:306	arg2	nigripes					250:257	Xylaria nigripes	242:257	Xylaria nigripes	242:257	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	2	24	used	used	303:306	arg2	fungus					282:287	a popular medicinal fungus	262:287	a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness	262:355	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	8	25	theme	polysaccharides	1291:1305	arg1	characteristics					1272:1286	the different physicochemical characteristics	1242:1286	the different physicochemical characteristics of polysaccharides from TXN and CXN	1242:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	5	26	theme	triple	679:684	arg1	conformation					692:703	triple helix conformation	679:703	triple helix conformation	679:703	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	6	27	theme	TXNP	825:828	arg1	glucose					848:854	glucose	848:854	glucose	848:854	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	6	27	theme	TXNP	825:828	arg1	composition					810:820	The monosaccharide composition	791:820	The monosaccharide composition of TXNP	791:828	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	2	28	theme	mental	342:347	arg1	illness					349:355	mental illness	342:355	mental illness	342:355	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	1	29	theme	mushroom	232:239	arg1	β-glucans					184:192	β-glucans	184:192	β-glucans	184:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	29	theme	mushroom	232:239	arg1	Polysaccharides					158:172	Polysaccharides	158:172	Polysaccharides including β-glucans	158:192	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	1	29	theme	mushroom	232:239	arg1	components					218:227	important bioactive components	198:227	important bioactive components of mushroom	198:239	Polysaccharides including β-glucans are important bioactive components of mushroom.
29763701	0	30	theme	soluble	21:27	arg1	polysaccharides					44:58	water soluble non-digestible polysaccharides	15:58	water soluble non-digestible polysaccharides	15:58	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	8	31	theme	similar	1181:1187	arg1	conditions					1189:1198	similar conditions	1181:1198	similar conditions of cultivation and extraction procedures	1181:1239	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	7	32	theme	lipopolysaccharide-induced	1068:1093	arg1	macrophages					1104:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	33	theme	NO	983:984	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	33	theme	NO	983:984	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	8	34	theme	extraction	1219:1228	arg1	procedures					1230:1239	extraction procedures	1219:1239	extraction procedures	1219:1239	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	7	35	theme	iNOS	1039:1042	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	35	theme	iNOS	1039:1042	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	5	36	dep	conformation	692:703	arg1	a					677:677	a	677:677	a	677:677	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	0	37	theme	nigripes	140:147	arg1	strains					149:155	two cultivated Xylaria nigripes strains	117:155	two cultivated Xylaria nigripes strains	117:155	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	7	38	from	production	969:978	arg1	macrophages					1104:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	39	from	expression	1025:1034	arg1	macrophages					1104:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	3	40	theme	polysaccharides	479:493	arg1	characteristics					398:412	physicochemical characteristics	382:412	physicochemical characteristics	382:412	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	40	theme	polysaccharides	479:493	arg1	activities					436:445	anti-inflammatory activities	418:445	anti-inflammatory activities	418:445	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	5	41	theme	molecular	752:760	arg1	14.7 kDa					781:788	14.7 kDa	781:788	14.7 kDa	781:788	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	41	theme	molecular	752:760	arg1	weight					762:767	the molecular weight	748:767	the molecular weight of CXNP	748:775	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	4	42	contain	possessed	624:632	arg2	spectra					659:665	relatively similar FT-IR spectra	634:665	relatively similar FT-IR spectra	634:665	Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra.
29763701	4	42	contain	possessed	624:632	arg1	CXNP					619:622	CXNP	619:622	CXNP	619:622	Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra.
29763701	4	42	contain	possessed	624:632	arg1	TXNP					610:613	TXNP	610:613	TXNP	610:613	Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra.
29763701	0	43	theme	non-digestible	29:42	arg1	polysaccharides					44:58	water soluble non-digestible polysaccharides	15:58	water soluble non-digestible polysaccharides	15:58	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	8	44	theme	procedures	1230:1239	arg1	conditions					1189:1198	similar conditions	1181:1198	similar conditions of cultivation and extraction procedures	1181:1239	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	3	45	from	characteristics	398:412	arg1	bodies					525:530	fruiting bodies	516:530	fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	516:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	2	46	theme	medicinal	272:280	arg1	fungus					282:287	a popular medicinal fungus	262:287	a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness	262:355	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	2	46	theme	medicinal	272:280	arg1	nigripes					250:257	Xylaria nigripes	242:257	Xylaria nigripes	242:257	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	3	47	theme	physicochemical	382:396	arg1	characteristics					398:412	physicochemical characteristics	382:412	physicochemical characteristics	382:412	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	0	48	theme	anti-inflammatory	64:80	arg1	activities					82:91	anti-inflammatory activities	64:91	anti-inflammatory activities of fruiting bodies	64:110	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	2	49	theme	popular	264:270	arg1	fungus					282:287	a popular medicinal fungus	262:287	a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness	262:355	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	2	49	theme	popular	264:270	arg1	nigripes					250:257	Xylaria nigripes	242:257	Xylaria nigripes	242:257	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	5	50	theme	853.8 kDa	729:737	arg1	conformation					692:703	triple helix conformation	679:703	triple helix conformation	679:703	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	5	50	theme	853.8 kDa	729:737	arg1	weight					719:724	molecular weight	709:724	molecular weight	709:724	TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa.
29763701	3	51	theme	X.	550:551	arg1	CXN					579:581	CXN	579:581	CXN	579:581	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	51	theme	X.	550:551	arg1	TXN					571:573	TXN	571:573	TXN	571:573	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	51	theme	X.	550:551	arg1	strains					562:568	two cultivated X. nigripes strains	535:568	two cultivated X. nigripes strains (TXN and CXN)	535:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	52	theme	non-digestible	464:477	arg1	CXNP					505:508	CXNP	505:508	CXNP	505:508	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	52	theme	non-digestible	464:477	arg1	TXNP					496:499	TXNP	496:499	TXNP	496:499	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	52	theme	non-digestible	464:477	arg1	polysaccharides					479:493	water soluble non-digestible polysaccharides	450:493	water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	450:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	7	53	theme	NF-κB	1055:1059	arg1	expression					1025:1034	the expression	1021:1034	the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages	1021:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	7	53	theme	NF-κB	1055:1059	arg1	production					969:978	the production	965:978	the production of NO, IL-1β, TNF-α and PGE2	965:1007	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	3	54	from	bodies	525:530	arg1	CXNP					505:508	CXNP	505:508	CXNP	505:508	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	54	from	bodies	525:530	arg1	polysaccharides					479:493	water soluble non-digestible polysaccharides	450:493	water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	450:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	54	from	bodies	525:530	arg1	TXNP					496:499	TXNP	496:499	TXNP	496:499	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	54	from	bodies	525:530	arg1	characteristics					398:412	physicochemical characteristics	382:412	physicochemical characteristics	382:412	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	54	from	bodies	525:530	arg1	activities					436:445	anti-inflammatory activities	418:445	anti-inflammatory activities	418:445	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	55	theme	nigripes	553:560	arg1	CXN					579:581	CXN	579:581	CXN	579:581	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	55	theme	nigripes	553:560	arg1	TXN					571:573	TXN	571:573	TXN	571:573	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	55	theme	nigripes	553:560	arg1	strains					562:568	two cultivated X. nigripes strains	535:568	two cultivated X. nigripes strains (TXN and CXN)	535:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	56	dep	characteristics	398:412	arg1	the					378:380	the	378:380	the	378:380	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	8	57	from	characteristics	1272:1286	arg1	TXN					1312:1314	TXN	1312:1314	TXN	1312:1314	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	8	57	from	characteristics	1272:1286	arg1	CXN					1320:1322	CXN	1320:1322	CXN	1320:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	4	58	theme	FT-IR	653:657	arg1	spectra					659:665	relatively similar FT-IR spectra	634:665	relatively similar FT-IR spectra	634:665	Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra.
29763701	7	59	theme	RAW264.7	1095:1102	arg1	macrophages					1104:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	the lipopolysaccharide-induced RAW264.7 macrophages	1064:1114	Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger.
29763701	2	60	theme	Xylaria	242:248	arg1	fungus					282:287	a popular medicinal fungus	262:287	a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness	262:355	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	2	60	theme	Xylaria	242:248	arg1	nigripes					250:257	Xylaria nigripes	242:257	Xylaria nigripes	242:257	Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness.
29763701	8	61	from	CXN	1320:1322	arg1	polysaccharides					1291:1305	polysaccharides	1291:1305	polysaccharides from TXN and CXN	1291:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	8	61	from	CXN	1320:1322	arg1	characteristics					1272:1286	the different physicochemical characteristics	1242:1286	the different physicochemical characteristics of polysaccharides from TXN and CXN	1242:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	3	62	theme	cultivated	539:548	arg1	CXN					579:581	CXN	579:581	CXN	579:581	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	62	theme	cultivated	539:548	arg1	TXN					571:573	TXN	571:573	TXN	571:573	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	62	theme	cultivated	539:548	arg1	strains					562:568	two cultivated X. nigripes strains	535:568	two cultivated X. nigripes strains (TXN and CXN)	535:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	8	63	theme	different	1246:1254	arg1	characteristics					1272:1286	the different physicochemical characteristics	1242:1286	the different physicochemical characteristics of polysaccharides from TXN and CXN	1242:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	4	64	theme	similar	645:651	arg1	spectra					659:665	relatively similar FT-IR spectra	634:665	relatively similar FT-IR spectra	634:665	Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra.
29763701	8	65	from	differences	1352:1362	arg1	potency					1391:1397	their anti-inflammatory potency	1367:1397	their anti-inflammatory potency	1367:1397	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	3	66	theme	fruiting	516:523	arg1	bodies					525:530	fruiting bodies	516:530	fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	516:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	6	67	contain	contained	870:878	arg2	glucose					900:906	glucose	900:906	glucose	900:906	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	6	67	contain	contained	870:878	arg2	mannose					888:894	mannose	888:894	mannose	888:894	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	6	67	contain	contained	870:878	arg2	xylose					880:885	xylose	880:885	xylose	880:885	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	6	67	contain	contained	870:878	arg1	CXNP					865:868	CXNP	865:868	CXNP	865:868	The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose.
29763701	0	68	from	strains	149:155	arg1	polysaccharides					44:58	water soluble non-digestible polysaccharides	15:58	water soluble non-digestible polysaccharides	15:58	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	0	68	from	strains	149:155	arg1	Differences					0:10	Differences	0:10	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.	0:156	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	0	68	from	strains	149:155	arg1	activities					82:91	anti-inflammatory activities	64:91	anti-inflammatory activities of fruiting bodies	64:110	Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains.
29763701	3	69	theme	soluble	456:462	arg1	CXNP					505:508	CXNP	505:508	CXNP	505:508	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	69	theme	soluble	456:462	arg1	TXNP					496:499	TXNP	496:499	TXNP	496:499	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	3	69	theme	soluble	456:462	arg1	polysaccharides					479:493	water soluble non-digestible polysaccharides	450:493	water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN)	450:582	This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN).
29763701	8	70	from	TXN	1312:1314	arg1	polysaccharides					1291:1305	polysaccharides	1291:1305	polysaccharides from TXN and CXN	1291:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
29763701	8	70	from	TXN	1312:1314	arg1	characteristics					1272:1286	the different physicochemical characteristics	1242:1286	the different physicochemical characteristics of polysaccharides from TXN and CXN	1242:1322	This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
30300696	2	0	theme	Scanning	379:386	arg1	microscopy					397:406	Scanning electron microscopy	379:406	Scanning electron microscopy	379:406	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	6	1	dep	hydrogels	958:966	arg1	we					968:969	we	968:969	we	968:969	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	4	2	theme	shape	786:790	arg1	range					792:796	different shape range	776:796	different shape range from 49% to 89%	776:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	2	3	theme	carboxymethyl	300:312	arg1	chitosan					314:321	carboxymethyl chitosan	300:321	carboxymethyl chitosan	300:321	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	3	4	theme	3D	575:576	arg1	printer					578:584	Hot-Melt extruded 3D printer	557:584	Hot-Melt extruded 3D printer	557:584	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	0	5	theme	Snakegourd	0:9	arg1	preparation					51:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	0	6	from	application	67:77	arg1	printing					85:92	3D printing	82:92	3D printing	82:92	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	6	7	theme	future	1009:1014	arg1	applications					1016:1027	future applications	1009:1027	future applications	1009:1027	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	4	8	theme	different	776:784	arg1	range					792:796	different shape range	776:796	different shape range from 49% to 89%	776:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	9	theme	acidic	678:683	arg1	conditions					697:706	acidic or alkaline conditions	678:706	conditions	697:706	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	2	10	theme	traditional	275:285	arg1	medicine					287:294	Chinese traditional medicine	267:294	Chinese traditional medicine	267:294	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	6	11	theme	delivery	1037:1044	arg1	system					1046:1051	drug delivery system	1032:1051	drug delivery system	1032:1051	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	0	12	theme	root/Astragalus	11:25	arg1	preparation					51:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	0	13	from	preparation	51:61	arg1	printing					85:92	3D printing	82:92	3D printing	82:92	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	5	14	theme	rectangular	902:912	arg1	shape					914:918	rectangular shape	902:918	rectangular shape	902:918	Therefore, there is a significant difference in the release between circular, cube and rectangular shape.
30300696	5	14	theme	rectangular	902:912	arg1	circular					883:890	circular	883:890	circular	883:890	Therefore, there is a significant difference in the release between circular, cube and rectangular shape.
30300696	2	15	theme	FTIR	409:412	arg1	analysis					414:421	FTIR analysis	409:421	FTIR analysis	409:421	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	2	16	theme	cytotoxicity	459:470	arg1	tests					472:476	cytotoxicity tests	459:476	cytotoxicity tests	459:476	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	3	17	with	patches	518:524	arg1	shapes					547:552	three different shapes	531:552	three different shapes	531:552	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	1	18	theme	good	110:113	arg1	retention					121:129	good water retention	110:129	good water retention	110:129	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	3	19	from	effects	602:608	arg1	release					637:643	the release	633:643	the release of drug	633:651	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	0	20	theme	hydrogel	42:49	arg1	preparation					51:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	4	21	theme	49	803:804	arg1	%					805:805	%	805:805	%	805:805	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	1	22	theme	water	115:119	arg1	retention					121:129	good water retention	110:129	good water retention	110:129	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	3	23	theme	different	613:621	arg1	shapes					623:628	different shapes	613:628	different shapes	613:628	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	1	24	theme	medical	200:206	arg1	industry					208:215	the medical industry	196:215	the medical industry	196:215	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	0	25	theme	polysaccharide	27:40	arg1	preparation					51:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation	0:61	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	4	26	with	patches	763:769	arg1	range					792:796	different shape range	776:796	different shape range from 49% to 89%	776:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	6	27	contain	have	980:983	arg2	potential					995:1003	excellent potential	985:1003	excellent potential for future applications	985:1027	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	6	27	contain	have	980:983	arg1	hydrogels					958:966	the hydrogels	954:966	the hydrogels we prepared	954:978	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	4	28	theme	alkaline	688:695	arg1	conditions					697:706	acidic or alkaline conditions	678:706	conditions	697:706	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	29	theme	patches	763:769	arg1	rate					736:739	the BSA cumulative release rate	709:739	the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%	709:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	2	30	theme	active	232:237	arg1	polysaccharide					239:252	active polysaccharide	232:252	active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel	232:351	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	6	31	theme	drug	1032:1035	arg1	system					1046:1051	drug delivery system	1032:1051	drug delivery system	1032:1051	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	5	32	theme	significant	837:847	arg1	difference					849:858	a significant difference	835:858	a significant difference in the release between circular, cube and rectangular shape	835:918	Therefore, there is a significant difference in the release between circular, cube and rectangular shape.
30300696	3	33	theme	drug	648:651	arg1	release					637:643	the release	633:643	the release of drug	633:651	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	3	34	theme	shapes	623:628	arg1	effects					602:608	the effects	598:608	the effects of different shapes on the release of drug	598:651	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	4	35	from	%	812:812	arg1	range					792:796	different shape range	776:796	different shape range from 49% to 89%	776:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	36	dep	%	805:805	arg1	to					807:808	to	807:808	to	807:808	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	37	theme	hydrogel	754:761	arg1	patches					763:769	the three hydrogel patches	744:769	the three hydrogel patches with different shape range from 49% to 89%	744:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	38	theme	BSA	713:715	arg1	rate					736:739	the BSA cumulative release rate	709:739	the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%	709:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	39	theme	89	810:811	arg1	%					805:805	%	805:805	%	805:805	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	1	40	contain	have	105:108	arg2	retention					121:129	good water retention	110:129	good water retention	110:129	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	1	40	contain	have	105:108	arg1	Hydrogels					95:103	Hydrogels	95:103	Hydrogels	95:103	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	1	40	contain	have	105:108	arg2	biodegradability					153:168	biodegradability	153:168	biodegradability	153:168	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	1	40	contain	have	105:108	arg2	biocompatibility					132:147	biocompatibility	132:147	biocompatibility	132:147	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	2	41	theme	Chinese	267:273	arg1	medicine					287:294	Chinese traditional medicine	267:294	Chinese traditional medicine	267:294	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	3	42	theme	composite	494:502	arg1	hydrogel					504:511	the composite hydrogel	490:511	the composite hydrogel into patches with three different shapes	490:552	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	6	43	theme	excellent	985:993	arg1	potential					995:1003	excellent potential	985:1003	excellent potential for future applications	985:1027	Through the study, we found that the hydrogels we prepared have excellent potential for future applications in drug delivery system.
30300696	3	44	theme	Hot-Melt	557:564	arg1	printer					578:584	Hot-Melt extruded 3D printer	557:584	Hot-Melt extruded 3D printer	557:584	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	3	45	theme	extruded	566:573	arg1	printer					578:584	Hot-Melt extruded 3D printer	557:584	Hot-Melt extruded 3D printer	557:584	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	4	46	theme	cumulative	717:726	arg1	rate					736:739	the BSA cumulative release rate	709:739	the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%	709:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	4	47	theme	release	728:734	arg1	rate					736:739	the BSA cumulative release rate	709:739	the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%	709:812	The results show, under acidic or alkaline conditions, the BSA cumulative release rate of the three hydrogel patches with different shape range from 49% to 89%.
30300696	1	48	used	used	188:191	arg2	they					174:177	they	174:177	they	174:177	Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry.
30300696	5	49	from	difference	849:858	arg1	release					867:873	the release	863:873	the release	863:873	Therefore, there is a significant difference in the release between circular, cube and rectangular shape.
30300696	3	50	theme	different	537:545	arg1	shapes					547:552	three different shapes	531:552	three different shapes	531:552	We printed the composite hydrogel into patches with three different shapes by Hot-Melt extruded 3D printer and studied the effects of different shapes on the release of drug.
30300696	2	51	contain	have	227:230	arg1	we					224:225	we	224:225	we	224:225	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	2	51	contain	have	227:230	arg2	polysaccharide					239:252	active polysaccharide	232:252	active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel	232:351	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
30300696	0	52	theme	3D	82:83	arg1	printing					85:92	3D printing	82:92	3D printing	82:92	Snakegourd root/Astragalus polysaccharide hydrogel preparation and application in 3D printing.
30300696	2	53	theme	electron	388:395	arg1	microscopy					397:406	Scanning electron microscopy	379:406	Scanning electron microscopy	379:406	Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation, release and cytotoxicity tests.
31667366	8	0	theme	therapeutic	1027:1037	arg1	antibody					1050:1057	a therapeutic monoclonal antibody	1025:1057	a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy	1025:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	8	0	theme	therapeutic	1027:1037	arg1	MW11-h317					1012:1020	MW11-h317	1012:1020	MW11-h317	1012:1020	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	3	1	theme	Monoclonal	304:313	arg1	drugs					330:334	Monoclonal antibody-based drugs	304:334	Monoclonal antibody-based drugs targeting PD-1 pathway	304:357	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	4	2	with	interactions	518:529	arg1	PD-L1/L2					536:543	PD-L1/L2	536:543	PD-L1/L2	536:543	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	0	3	theme	Asn58	85:89	arg1	glycosylation					91:103	PD-1 Asn58 glycosylation	80:103	PD-1 Asn58 glycosylation	80:103	Identification of a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation.
31667366	7	4	theme	PD-1	944:947	arg1	antibodies					949:958	the first two approved clinical PD-1 antibodies	912:958	the first two approved clinical PD-1 antibodies	912:958	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	4	theme	PD-1	944:947	arg1	pembrolizumab					975:987	pembrolizumab	975:987	pembrolizumab	975:987	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	4	theme	PD-1	944:947	arg1	nivolumab					961:969	nivolumab	961:969	nivolumab	961:969	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	0	5	theme	PD-1	80:83	arg1	glycosylation					91:103	PD-1 Asn58 glycosylation	80:103	PD-1 Asn58 glycosylation	80:103	Identification of a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation.
31667366	8	6	theme	monoclonal	1039:1048	arg1	antibody					1050:1057	a therapeutic monoclonal antibody	1025:1057	a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy	1025:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	8	6	theme	monoclonal	1039:1048	arg1	MW11-h317					1012:1020	MW11-h317	1012:1020	MW11-h317	1012:1020	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	6	7	theme	Asn58	804:808	arg1	glycosylation					810:822	Asn58 glycosylation	804:822	Asn58 glycosylation	804:822	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	7	8	theme	glycan	858:863	arg1	different					897:905	different	897:905	different	897:905	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	8	theme	glycan	858:863	arg1	epitope					865:871	The unique glycan epitope	847:871	The unique glycan epitope in PD-1 to MW11-h317	847:892	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	8	9	theme	PD-1	1062:1065	arg1	glycosylation-targeting					1067:1089	PD-1 glycosylation-targeting	1062:1089	PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy	1062:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	5	10	theme	tumor	623:627	arg1	growth					629:634	tumor growth	623:634	tumor growth in mouse model	623:649	MW11-h317 can effectively induce T-cell-mediated immune response and inhibit tumor growth in mouse model.
31667366	1	11	theme	inhibitory	140:149	arg1	receptor					151:158	inhibitory receptor	140:158	inhibitory receptor	140:158	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	3	12	theme	PD-1	346:349	arg1	pathway					351:357	PD-1 pathway	346:357	PD-1 pathway	346:357	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	3	13	from	promise	380:386	arg1	therapy					398:404	cancer therapy	391:404	cancer therapy	391:404	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	7	14	theme	approved	926:933	arg1	antibodies					949:958	the first two approved clinical PD-1 antibodies	912:958	the first two approved clinical PD-1 antibodies	912:958	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	14	theme	approved	926:933	arg1	pembrolizumab					975:987	pembrolizumab	975:987	pembrolizumab	975:987	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	14	theme	approved	926:933	arg1	nivolumab					961:969	nivolumab	961:969	nivolumab	961:969	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	5	15	theme	mouse	639:643	arg1	model					645:649	mouse model	639:649	mouse model	639:649	MW11-h317 can effectively induce T-cell-mediated immune response and inhibit tumor growth in mouse model.
31667366	7	16	theme	clinical	935:942	arg1	antibodies					949:958	the first two approved clinical PD-1 antibodies	912:958	the first two approved clinical PD-1 antibodies	912:958	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	16	theme	clinical	935:942	arg1	pembrolizumab					975:987	pembrolizumab	975:987	pembrolizumab	975:987	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	16	theme	clinical	935:942	arg1	nivolumab					961:969	nivolumab	961:969	nivolumab	961:969	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	17	theme	unique	851:856	arg1	different					897:905	different	897:905	different	897:905	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	17	theme	unique	851:856	arg1	epitope					865:871	The unique glycan epitope	847:871	The unique glycan epitope in PD-1 to MW11-h317	847:892	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	8	18	theme	glycosylation-targeting	1067:1089	arg1	antibody					1050:1057	a therapeutic monoclonal antibody	1025:1057	a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy	1025:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	8	18	theme	glycosylation-targeting	1067:1089	arg1	MW11-h317					1012:1020	MW11-h317	1012:1020	MW11-h317	1012:1020	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	5	19	theme	T-cell-mediated	579:593	arg1	response					602:609	T-cell-mediated immune response	579:609	T-cell-mediated immune response	579:609	MW11-h317 can effectively induce T-cell-mediated immune response and inhibit tumor growth in mouse model.
31667366	2	20	theme	antitumor	284:292	arg1	response					294:301	active T-cell-mediated antitumor response	261:301	active T-cell-mediated antitumor response	261:301	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	1	21	theme	immune	164:169	arg1	protein					182:188	immune checkpoint protein	164:188	immune checkpoint protein	164:188	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	0	22	theme	monoclonal	20:29	arg1	antibody					31:38	a monoclonal antibody	18:38	a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation	18:103	Identification of a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation.
31667366	7	23	theme	first	916:920	arg1	antibodies					949:958	the first two approved clinical PD-1 antibodies	912:958	the first two approved clinical PD-1 antibodies	912:958	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	23	theme	first	916:920	arg1	pembrolizumab					975:987	pembrolizumab	975:987	pembrolizumab	975:987	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	23	theme	first	916:920	arg1	nivolumab					961:969	nivolumab	961:969	nivolumab	961:969	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	5	24	theme	immune	595:600	arg1	response					602:609	T-cell-mediated immune response	579:609	T-cell-mediated immune response	579:609	MW11-h317 can effectively induce T-cell-mediated immune response and inhibit tumor growth in mouse model.
31667366	2	25	theme	T-cell-mediated	268:282	arg1	response					294:301	active T-cell-mediated antitumor response	261:301	active T-cell-mediated antitumor response	261:301	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	6	26	theme	complex	692:698	arg1	structure					660:668	Crystal structure	652:668	Crystal structure of PD-1/MW11-h317 Fab complex	652:698	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	1	27	theme	checkpoint	171:180	arg1	protein					182:188	immune checkpoint protein	164:188	immune checkpoint protein	164:188	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	3	28	theme	great	374:378	arg1	promise					380:386	great promise	374:386	great promise in cancer therapy	374:404	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	5	29	from	growth	629:634	arg1	model					645:649	mouse model	639:649	mouse model	639:649	MW11-h317 can effectively induce T-cell-mediated immune response and inhibit tumor growth in mouse model.
31667366	2	30	theme	active	261:266	arg1	response					294:301	active T-cell-mediated antitumor response	261:301	active T-cell-mediated antitumor response	261:301	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	6	31	theme	Fab	688:690	arg1	complex					692:698	PD-1/MW11-h317 Fab complex	673:698	PD-1/MW11-h317 Fab complex	673:698	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	2	32	theme	PD-1	219:222	arg1	interaction					204:214	the interaction	200:214	the interaction of PD-1 and its ligands PD-L1/ L2	200:248	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	6	33	theme	PD-1/MW11-h317	673:686	arg1	complex					692:698	PD-1/MW11-h317 Fab complex	673:698	PD-1/MW11-h317 Fab complex	673:698	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	2	34	dep	PD-1	219:222	arg1	L2					247:248	PD-L1/ L2	240:248	PD-L1/ L2	240:248	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	0	35	theme	antibody	31:38	arg1	Identification					0:13	Identification	0:13	Identification of a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation.	0:104	Identification of a monoclonal antibody that targets PD-1 in a manner requiring PD-1 Asn58 glycosylation.
31667366	4	36	theme	PD-1	513:516	arg1	interactions					518:529	PD-1 interactions	513:529	PD-1 interactions with PD-L1/L2	513:543	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	8	37	theme	efficient	1108:1116	arg1	alternative					1118:1128	efficient alternative	1108:1128	efficient alternative for cancer therapy	1108:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	4	38	theme	monoclonal	449:458	arg1	antibody					460:467	an anti-PD-1 monoclonal antibody	436:467	an anti-PD-1 monoclonal antibody	436:467	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	4	38	theme	monoclonal	449:458	arg1	MW11-h317					425:433	MW11-h317	425:433	MW11-h317	425:433	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	3	39	theme	cancer	391:396	arg1	therapy					398:404	cancer therapy	391:404	cancer therapy	391:404	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	8	40	theme	cancer	1134:1139	arg1	therapy					1141:1147	cancer therapy	1134:1147	cancer therapy	1134:1147	These results suggest MW11-h317 as a therapeutic monoclonal antibody of PD-1 glycosylation-targeting which may become efficient alternative for cancer therapy.
31667366	4	41	theme	anti-PD-1	439:447	arg1	antibody					460:467	an anti-PD-1 monoclonal antibody	436:467	an anti-PD-1 monoclonal antibody	436:467	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	4	41	theme	anti-PD-1	439:447	arg1	MW11-h317					425:433	MW11-h317	425:433	MW11-h317	425:433	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	4	42	theme	high	479:482	arg1	affinity					484:491	high affinity	479:491	high affinity for PD-1	479:500	Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for PD-1 and blocks PD-1 interactions with PD-L1/L2.
31667366	1	43	theme	Programmed	106:115	arg1	death					122:126	Programmed cell death 1	106:128	Programmed cell death 1 (PD-1)	106:135	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	1	43	theme	Programmed	106:115	arg1	PD-1					131:134	PD-1	131:134	PD-1	131:134	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	1	44	theme	cell	117:120	arg1	death					122:126	Programmed cell death 1	106:128	Programmed cell death 1 (PD-1)	106:135	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	1	44	theme	cell	117:120	arg1	PD-1					131:134	PD-1	131:134	PD-1	131:134	Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein.
31667366	7	45	dep	MW11-h317	884:892	arg1	to					881:882	to	881:882	to	881:882	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	2	46	theme	PD-L1/	240:245	arg1	L2					247:248	PD-L1/ L2	240:248	PD-L1/ L2	240:248	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	6	47	theme	critical	832:839	arg1	role					841:844	a critical role	830:844	a critical role	830:844	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	2	48	theme	ligands	232:238	arg1	interaction					204:214	the interaction	200:214	the interaction of PD-1 and its ligands PD-L1/ L2	200:248	Blocking the interaction of PD-1 and its ligands PD-L1/ L2 is able to active T-cell-mediated antitumor response.
31667366	6	49	theme	PD-1	749:752	arg1	glycosylation					732:744	glycosylation	732:744	glycosylation of PD-1	732:752	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	6	49	theme	PD-1	749:752	arg1	loops					722:726	the loops	718:726	the loops	718:726	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	6	50	theme	Crystal	652:658	arg1	structure					660:668	Crystal structure	652:668	Crystal structure of PD-1/MW11-h317 Fab complex	652:698	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	3	51	theme	antibody-based	315:328	arg1	drugs					330:334	Monoclonal antibody-based drugs	304:334	Monoclonal antibody-based drugs targeting PD-1 pathway	304:357	Monoclonal antibody-based drugs targeting PD-1 pathway have exhibited great promise in cancer therapy.
31667366	7	52	from	epitope	865:871	arg1	MW11-h317					884:892	MW11-h317	884:892	MW11-h317	884:892	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	53	from	antibodies	949:958	arg1	different					897:905	different	897:905	different	897:905	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	7	53	from	antibodies	949:958	arg1	epitope					865:871	The unique glycan epitope	847:871	The unique glycan epitope in PD-1 to MW11-h317	847:892	The unique glycan epitope in PD-1 to MW11-h317 is different from the first two approved clinical PD-1 antibodies, nivolumab and pembrolizumab.
31667366	6	54	gly	glycosylation	732:744	arg1	PD-1					749:752	PD-1	749:752	PD-1	749:752	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	6	54	gly	glycosylation	732:744	arg1	binding					786:792	binding	786:792	binding	786:792	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
31667366	6	54	gly	glycosylation	732:744	arg1	recognition					770:780	recognition	770:780	recognition	770:780	Crystal structure of PD-1/MW11-h317 Fab complex reveals that both the loops and glycosylation of PD-1 are involved in recognition and binding, in which Asn58 glycosylation plays a critical role.
30481221	7	0	dep	activity	1627:1634	arg1	DPPH					1637:1640	DPPH	1637:1640	DPPH	1637:1640	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	11	1	theme	nutraceutical	2189:2201	arg1	industries					2222:2231	nutraceutical and pharmaceutical industries	2189:2231	nutraceutical and pharmaceutical industries	2189:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	11	2	theme	bioactive	2084:2092	arg1	compounds					2094:2102	bioactive compounds	2084:2102	bioactive compounds	2084:2102	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	9	3	dep	showed	1787:1792	arg1	5.2					1864:1866	5.2	1864:1866	5.2	1864:1866	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	3	dep	showed	1787:1792	arg1	3.1					1857:1859	3.1	1857:1859	3.1	1857:1859	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	7	4	dep	DPPH	1637:1640	arg1	IC50					1656:1659	6 vs 18 mg/L IC50	1643:1659	6 vs 18 mg/L IC50 for the ethanol extract	1643:1683	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	5	theme	Bark	1452:1455	arg1	activity					1469:1476	Bark antioxidant activity	1452:1476	Bark antioxidant activity	1452:1476	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	1	6	theme	extractives	279:289	arg1	characteristics					195:209	the chemical and thermal characteristics	170:209	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	6	theme	extractives	279:289	arg1	fractions					395:403	different bark fractions	380:403	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	6	theme	extractives	279:289	arg1	potential					256:264	the phytochemical and antioxidant potential	222:264	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	3	7	dep	mineral	820:826	arg1	%					840:840	0.4%	837:840	0.4%	837:840	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	7	dep	mineral	820:826	arg1	%					864:864	20.3%	860:864	20.3%	860:864	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	7	dep	mineral	820:826	arg1	%					872:872	3.8%	869:872	3.8%	869:872	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	7	dep	mineral	820:826	arg1	%					832:832	3.9%	829:832	3.9%	829:832	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	5	8	theme	fractions	1144:1152	arg1	size					1127:1130	particle size	1118:1130	particle size of the bark fractions	1118:1152	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	7	9	dep	IC50	1656:1659	arg1	mg/L					1651:1654	mg/L	1651:1654	mg/L	1651:1654	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	10	theme	radical	1608:1614	arg1	scavenging					1616:1625	free radical scavenging	1603:1625	free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract)	1603:1684	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	4	11	theme	bark	934:937	arg1	fractions					939:947	bark fractions	934:947	bark fractions	934:947	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	9	12	theme	lower	1801:1805	arg1	volatiles					1807:1815	a much lower volatiles	1794:1815	a much lower volatiles to fixed carbon ratio (V/FC)	1794:1844	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	1	13	theme	bark	390:393	arg1	fractions					395:403	different bark fractions	380:403	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	14	theme	flavonoids	1354:1363	arg1	wood					1319:1322	wood	1319:1322	wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher)	1319:1402	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	9	15	theme	carbon	1826:1831	arg1	V/FC					1840:1843	V/FC	1840:1843	V/FC	1840:1843	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	15	theme	carbon	1826:1831	arg1	ratio					1833:1837	fixed carbon ratio	1820:1837	fixed carbon ratio (V/FC)	1820:1844	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	3	16	dep	higher	813:818	arg1	extractives					847:857	extractives	847:857	extractives	847:857	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	16	dep	higher	813:818	arg1	mineral					820:826	mineral	820:826	mineral	820:826	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	16	dep	higher	813:818	arg1	polysaccharides					885:899	lower polysaccharides	879:899	lower polysaccharides (48% vs 71%)	879:912	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	1	17	theme	mechanical	417:426	arg1	fractionation					428:440	mechanical fractionation	417:440	mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	417:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	18	theme	water	1264:1268	arg1	extracts					1270:1277	water extracts	1264:1277	water extracts	1264:1277	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	2	19	dep	possible	604:611	arg1	uses					613:616	uses	613:616	uses	613:616	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	11	20	theme	production	2111:2120	arg1	potential					2122:2130	the production potential	2107:2130	the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2107:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	11	21	with	extractives	2067:2077	arg1	compounds					2094:2102	bioactive compounds	2084:2102	bioactive compounds	2084:2102	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	11	21	with	extractives	2067:2077	arg1	potential					2122:2130	the production potential	2107:2130	the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2107:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	6	22	theme	ethanol	1252:1258	arg1	profile					1241:1247	The phytochemical profile	1223:1247	The phytochemical profile of ethanol and water extracts	1223:1277	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	6	23	dep	tannins	1381:1387	arg1	higher					1396:1401	higher	1396:1401	higher	1396:1401	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	1	24	dep	abies	304:308	arg1	bark					310:313	bark	310:313	the Picea abies bark from an industrial mill	294:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	2	25	theme	size	682:685	arg1	process					697:703	an initial size reduction process	671:703	an initial size reduction process to achieve fractions with different characteristics	671:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	11	26	theme	possible	2009:2016	arg1	upgrade					2018:2024	their possible upgrade	2003:2024	their possible upgrade	2003:2024	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	2	27	dep	bark	579:582	arg1	determine					594:602	determine	594:602	to better determine possible uses other than burning for energy production	584:657	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	2	27	dep	bark	579:582	arg1	test					666:669	test	666:669	to test an initial size reduction process to achieve fractions with different characteristics	663:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	2	28	theme	abies	573:577	arg1	bark					579:582	the Picea abies bark	563:582	the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics	563:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	0	29	theme	thermal	94:100	arg1	properties					102:111	thermal properties	94:111	thermal properties	94:111	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	7	30	theme	ethanol	1582:1588	arg1	extract					1590:1596	the ethanol extract	1578:1596	the ethanol extract	1578:1596	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	6	31	theme	total	1327:1331	arg1	phenols					1333:1339	total phenols	1327:1339	total phenols (2x higher)	1327:1351	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	4	32	dep	fraction	1036:1043	arg1	%					1054:1054	19%	1052:1054	19%	1052:1054	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	4	32	dep	fraction	1036:1043	arg1	%					1062:1062	25%	1060:1062	25%	1060:1062	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	4	32	dep	fraction	1036:1043	arg1	%					1049:1049	3.9%	1046:1049	3.9%	1046:1049	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	1	33	from	mill	334:337	arg1	bark					310:313	bark	310:313	the Picea abies bark from an industrial mill	294:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	34	theme	tannins	1381:1387	arg1	wood					1319:1322	wood	1319:1322	wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher)	1319:1402	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	11	35	theme	chemical	1971:1978	arg1	features					1980:1987	chemical features	1971:1987	chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	1971:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	0	36	dep	composition	81:91	arg1	activity					132:139	activity	132:139	activity	132:139	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	1	37	theme	Picea	298:302	arg1	abies					304:308	the Picea abies bark from an industrial mill	294:337	the Picea abies bark from an industrial mill	294:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	37	theme	Picea	298:302	arg1	components					360:369	their wood and bark components	340:369	their wood and bark components	340:369	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	38	theme	particle	1437:1444	arg1	size					1446:1449	particle size	1437:1449	particle size	1437:1449	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	9	39	theme	same	1882:1885	arg1	value					1902:1906	the same higher heating value	1878:1906	the same higher heating value (20.3 MJ/kg)	1878:1919	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	39	theme	same	1882:1885	arg1	MJ/kg					1914:1918	20.3 MJ/kg	1909:1918	20.3 MJ/kg	1909:1918	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	0	40	theme	Picea	34:38	arg1	abies					40:44	Picea abies	34:44	Picea abies industrial bark	34:60	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	8	41	theme	bark	1709:1712	arg1	fractions					1714:1722	The different bark fractions	1695:1722	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity was very similar.
30481221	5	42	theme	particle	1118:1125	arg1	size					1127:1130	particle size	1118:1130	particle size of the bark fractions	1118:1152	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	9	43	theme	heating	1894:1900	arg1	value					1902:1906	the same higher heating value	1878:1906	the same higher heating value (20.3 MJ/kg)	1878:1919	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	43	theme	heating	1894:1900	arg1	MJ/kg					1914:1918	20.3 MJ/kg	1909:1918	20.3 MJ/kg	1909:1918	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	0	44	theme	industrial	46:55	arg1	bark					57:60	Picea abies industrial bark	34:60	Picea abies industrial bark	34:60	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	8	45	theme	antioxidant	1724:1734	arg1	activity					1736:1743	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity was very similar.
30481221	2	46	theme	different	731:739	arg1	characteristics					741:755	different characteristics	731:755	different characteristics	731:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	2	47	from	knowledge	550:558	arg1	bark					579:582	the Picea abies bark	563:582	the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics	563:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	5	48	theme	relative	1201:1208	arg1	composition					1210:1220	the same relative composition	1192:1220	the same relative composition	1192:1220	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	11	49	link	hemicellulose-derived	2136:2156	arg1	oligomers					2158:2166	hemicellulose-derived oligomers	2136:2166	hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2136:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	1	50	theme	phytochemical	226:238	arg1	potential					256:264	the phytochemical and antioxidant potential	222:264	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	7	51	theme	ferric-reducing	1511:1525	arg1	power					1539:1543	ferric-reducing antioxidant power	1511:1543	ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract)	1511:1597	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	11	52	theme	possible	2173:2180	arg1	use					2182:2184	possible use	2173:2184	possible use in nutraceutical and pharmaceutical industries	2173:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	5	53	theme	Polysaccharide	1079:1092	arg1	contents					1094:1101	Polysaccharide contents	1079:1101	Polysaccharide contents	1079:1101	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	1	54	theme	antioxidant	244:254	arg1	potential					256:264	the phytochemical and antioxidant potential	222:264	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	7	55	theme	ethanol	1669:1675	arg1	extract					1677:1683	the ethanol extract	1665:1683	the ethanol extract	1665:1683	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	56	theme	antioxidant	1457:1467	arg1	activity					1469:1476	Bark antioxidant activity	1452:1476	Bark antioxidant activity	1452:1476	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	1	57	theme	coarse	498:503	arg1	B6					505:506	coarse B6	498:506	coarse B6	498:506	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	mm					491:492	0.450 < Φ<0.850 mm	475:492	0.450 < Φ<0.850 mm	475:492	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	B1					448:449	fine B1	443:449	fine B1	443:449	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	mm					518:519	2 < Φ<10 mm	509:519	2 < Φ<10 mm	509:519	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	B3					471:472	medium B3	464:472	medium B3	464:472	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	B6					505:506	coarse B6	498:506	coarse B6	498:506	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	58	dep	fractionation	428:440	arg1	mm					460:461	Φ<0.180 mm	452:461	Φ<0.180 mm	452:461	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	59	theme	fine	443:446	arg1	B1					448:449	fine B1	443:449	fine B1	443:449	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	59	theme	fine	443:446	arg1	mm					518:519	2 < Φ<10 mm	509:519	2 < Φ<10 mm	509:519	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	3	60	dep	polysaccharides	885:899	arg1	%					904:904	48%	902:904	48%	902:904	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	60	dep	polysaccharides	885:899	arg1	%					911:911	71%	909:911	71%	909:911	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	1	61	theme	polar	273:277	arg1	extractives					279:289	the polar extractives	269:289	the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components	269:369	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	2	62	theme	initial	674:680	arg1	process					697:703	an initial size reduction process	671:703	an initial size reduction process to achieve fractions with different characteristics	671:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	6	63	theme	phytochemical	1227:1239	arg1	profile					1241:1247	The phytochemical profile	1223:1247	The phytochemical profile of ethanol and water extracts	1223:1277	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	1	64	theme	chemical	174:181	arg1	characteristics					195:209	the chemical and thermal characteristics	170:209	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	3	65	theme	lower	879:883	arg1	polysaccharides					885:899	lower polysaccharides	879:899	lower polysaccharides (48% vs 71%)	879:912	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	4	66	theme	higher	966:971	arg1	ash					973:975	higher ash	966:975	higher ash (6.3%)	966:982	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	4	66	theme	higher	966:971	arg1	%					981:981	6.3%	978:981	6.3%	978:981	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	9	67	theme	thermal	1768:1774	arg1	properties					1776:1785	thermal properties	1768:1785	Bark thermal properties	1763:1785	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	5	68	theme	bark	1139:1142	arg1	fractions					1144:1152	the bark fractions	1135:1152	the bark fractions	1135:1152	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	1	69	theme	thermal	187:193	arg1	characteristics					195:209	the chemical and thermal characteristics	170:209	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	2	70	with	fractions	716:724	arg1	characteristics					741:755	different characteristics	731:755	different characteristics	731:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	1	71	theme	medium	464:469	arg1	B3					471:472	medium B3	464:472	medium B3	464:472	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	72	theme	higher	1369:1374	arg1	flavonoids					1354:1363	flavonoids	1354:1363	flavonoids (3x higher)	1354:1375	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	2	73	theme	reduction	687:695	arg1	process					697:703	an initial size reduction process	671:703	an initial size reduction process to achieve fractions with different characteristics	671:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	1	74	theme	different	380:388	arg1	fractions					395:403	different bark fractions	380:403	the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm)	170:520	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	7	75	theme	scavenging	1616:1625	arg1	activity					1627:1634	free radical scavenging activity	1603:1634	free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract)	1603:1684	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	9	76	dep	Bark	1763:1766	arg1	properties					1776:1785	thermal properties	1768:1785	Bark thermal properties	1763:1785	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	6	77	theme	higher	1289:1294	arg1	contents					1296:1303	higher contents	1289:1303	higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher)	1289:1402	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	7	78	theme	free	1603:1606	arg1	scavenging					1616:1625	free radical scavenging	1603:1625	free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract)	1603:1684	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	9	79	theme	fixed	1820:1824	arg1	V/FC					1840:1843	V/FC	1840:1843	V/FC	1840:1843	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	79	theme	fixed	1820:1824	arg1	ratio					1833:1837	fixed carbon ratio	1820:1837	fixed carbon ratio (V/FC)	1820:1844	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	6	80	theme	extracts	1270:1277	arg1	profile					1241:1247	The phytochemical profile	1223:1247	The phytochemical profile of ethanol and water extracts	1223:1277	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	7	81	dep	mg/L	1651:1654	arg1	18					1648:1649	18	1648:1649	18	1648:1649	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	81	dep	mg/L	1651:1654	arg1	6					1643:1643	6	1643:1643	6	1643:1643	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	6	82	theme	higher	1345:1350	arg1	2x					1342:1343	2x	1342:1343	2x higher	1342:1350	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	0	83	theme	chemical	72:79	arg1	composition					81:91	chemical composition	72:91	chemical composition	72:91	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	1	84	theme	present	146:152	arg1	work					154:157	The present work	142:157	The present work	142:157	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	2	85	theme	energy	641:646	arg1	production					648:657	energy production	641:657	energy production	641:657	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	1	86	theme	wood	346:349	arg1	abies					304:308	the Picea abies bark from an industrial mill	294:337	the Picea abies bark from an industrial mill	294:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	86	theme	wood	346:349	arg1	components					360:369	their wood and bark components	340:369	their wood and bark components	340:369	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	87	theme	phenols	1333:1339	arg1	wood					1319:1322	wood	1319:1322	wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher)	1319:1402	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	3	88	theme	lignin	799:804	arg1	contents					914:921	similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents	791:921	similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents	791:921	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	11	89	theme	high	2062:2065	arg1	extractives					2067:2077	the high extractives	2058:2077	the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2058:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	2	90	theme	Picea	567:571	arg1	abies					573:577	Picea abies	567:577	the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics	563:755	The aim is to increase the knowledge on the Picea abies bark to better determine possible uses other than burning for energy production and to test an initial size reduction process to achieve fractions with different characteristics.
30481221	0	91	theme	polar	117:121	arg1	extracts					123:130	polar extracts	117:130	polar extracts	117:130	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	1	92	theme	bark	355:358	arg1	abies					304:308	the Picea abies bark from an industrial mill	294:337	the Picea abies bark from an industrial mill	294:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	1	92	theme	bark	355:358	arg1	components					360:369	their wood and bark components	340:369	their wood and bark components	340:369	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	7	93	dep	power	1539:1543	arg1	methods					1686:1692	methods	1686:1692	methods	1686:1692	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	93	dep	power	1539:1543	arg1	FRAP					1546:1549	FRAP	1546:1549	FRAP	1546:1549	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	7	93	dep	power	1539:1543	arg1	mmolFe2+/gExt					1560:1572	10 vs 6 mmolFe2+/gExt	1552:1572	10 vs 6 mmolFe2+/gExt for the ethanol extract	1552:1596	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	11	94	dep	point	1994:1998	arg1	taking					2038:2043	taking	2038:2043	taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2038:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	0	95	theme	integrated	3:12	arg1	characterization					14:29	An integrated characterization	0:29	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.	0:140	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	3	96	dep	lignin	799:804	arg1	%					809:809	27%	807:809	27%	807:809	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	96	dep	lignin	799:804	arg1	higher					813:818	higher	813:818	higher	813:818	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	3	96	dep	lignin	799:804	arg1	similar					791:797	similar	791:797	similar	791:797	Compared to wood, bark presented similar lignin (27%), higher mineral (3.9% vs 0.4%) and extractives (20.3% vs 3.8%) and lower polysaccharides (48% vs 71%) contents.
30481221	11	97	theme	extractives	2067:2077	arg1	advantage					2045:2053	advantage	2045:2053	advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2045:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	11	98	from	use	2182:2184	arg1	industries					2222:2231	nutraceutical and pharmaceutical industries	2189:2231	nutraceutical and pharmaceutical industries	2189:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	5	99	dep	increased	1103:1111	arg1	%					1157:1157	38%	1155:1157	38%	1155:1157	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	5	99	dep	increased	1103:1111	arg1	%					1164:1164	52%	1162:1164	52% for B1 and B6	1162:1178	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	8	100	theme	different	1699:1707	arg1	fractions					1714:1722	The different bark fractions	1695:1722	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity was very similar.
30481221	11	101	with	oligomers	2158:2166	arg1	use					2182:2184	possible use	2173:2184	possible use in nutraceutical and pharmaceutical industries	2173:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	11	102	theme	pharmaceutical	2207:2220	arg1	industries					2222:2231	nutraceutical and pharmaceutical industries	2189:2231	nutraceutical and pharmaceutical industries	2189:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30481221	6	103	with	tendency	1423:1430	arg1	size					1446:1449	particle size	1437:1449	particle size	1437:1449	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	0	104	theme	abies	40:44	arg1	bark					57:60	Picea abies industrial bark	34:60	Picea abies industrial bark	34:60	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	1	105	theme	abies	304:308	arg1	extractives					279:289	the polar extractives	269:289	the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components	269:369	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	6	106	dep	phenols	1333:1339	arg1	2x					1342:1343	2x	1342:1343	2x higher	1342:1350	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	8	107	theme	fractions	1714:1722	arg1	activity					1736:1743	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity	1695:1743	The different bark fractions antioxidant activity was very similar.
30481221	9	108	theme	higher	1887:1892	arg1	value					1902:1906	the same higher heating value	1878:1906	the same higher heating value (20.3 MJ/kg)	1878:1919	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	9	108	theme	higher	1887:1892	arg1	MJ/kg					1914:1918	20.3 MJ/kg	1909:1918	20.3 MJ/kg	1909:1918	Bark thermal properties showed a much lower volatiles to fixed carbon ratio (V/FC) than wood (3.1 vs 5.2) although the same higher heating value (20.3 MJ/kg).
30481221	0	109	theme	bark	57:60	arg1	characterization					14:29	An integrated characterization	0:29	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.	0:140	An integrated characterization of Picea abies industrial bark regarding chemical composition, thermal properties and polar extracts activity.
30481221	7	110	theme	antioxidant	1527:1537	arg1	power					1539:1543	ferric-reducing antioxidant power	1511:1543	ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract)	1511:1597	Bark antioxidant activity was higher than that of wood for ferric-reducing antioxidant power (FRAP, 10 vs 6 mmolFe2+/gExt for the ethanol extract) and free radical scavenging activity (DPPH, 6 vs 18 mg/L IC50 for the ethanol extract) methods.
30481221	6	111	theme	increasing	1412:1421	arg1	tendency					1423:1430	an increasing tendency	1409:1430	an increasing tendency with particle size	1409:1449	The phytochemical profile of ethanol and water extracts presented higher contents for bark than wood of total phenols (2x higher), flavonoids (3x higher) and tannins (4-10x higher) with an increasing tendency with particle size.
30481221	5	112	theme	same	1196:1199	arg1	composition					1210:1220	the same relative composition	1192:1220	the same relative composition	1192:1220	Polysaccharide contents increased with particle size of the bark fractions (38% vs 52% for B1 and B6) but showed the same relative composition.
30481221	1	113	theme	industrial	323:332	arg1	mill					334:337	an industrial mill	320:337	an industrial mill	320:337	The present work determines the chemical and thermal characteristics as well as the phytochemical and antioxidant potential of the polar extractives of the Picea abies bark from an industrial mill, their wood and bark components and also different bark fractions obtained by mechanical fractionation (fine B1, Φ<0.180 mm, medium B3, 0.450 < Φ<0.850 mm and coarse B6, 2 < Φ<10 mm).
30481221	4	114	theme	coarse	1029:1034	arg1	fraction					1036:1043	the coarse fraction	1025:1043	the coarse fraction (3.9%, 19% and 25% respectively)	1025:1076	Regarding bark fractions the fines showed higher ash (6.3%), extractives (25%) and lignin (29%) than the coarse fraction (3.9%, 19% and 25% respectively).
30481221	11	115	theme	hemicellulose-derived	2136:2156	arg1	oligomers					2158:2166	hemicellulose-derived oligomers	2136:2166	hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries	2136:2231	Bark presented chemical features that point to their possible upgrade, whether by taking advantage of the high extractives with bioactive compounds or the production potential for hemicellulose-derived oligomers with possible use in nutraceutical and pharmaceutical industries.
30361022	0	0	theme	oil	91:93	arg1	encapsulation					40:52	encapsulation	40:52	encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion	40:113	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	2	1	theme	Higher	552:557	arg1	efficiency					573:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	1	2	theme	double	162:167	arg1	emulsions					169:177	The double emulsions	158:177	The double emulsions	158:177	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	0	3	theme	fish	86:89	arg1	oil					91:93	fish oil	86:93	fish oil	86:93	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	7	4	theme	significant	1053:1063	arg1	differences					1065:1075	No significant differences	1050:1075	No significant differences	1050:1075	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	1	5	theme	physical	381:388	arg1	characteristics					390:404	physical characteristics	381:404	physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …)	381:486	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	4	6	theme	SFA	819:821	arg1	values					800:805	The higher values	789:805	The higher values of PUFA and SFA	789:821	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	0	7	theme	W1/O/W2	98:104	arg1	emulsion					106:113	W1/O/W2 emulsion	98:113	W1/O/W2 emulsion	98:113	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	1	8	theme	characteristics	390:404	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	3	9	theme	wall	774:777	arg1	material					779:786	other wall material	768:786	other wall material	768:786	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	1	10	theme	inulin	337:342	arg1	Fuc					364:366	Fuc	364:366	Fuc	364:366	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	10	theme	inulin	337:342	arg1	Inu					345:347	inulin (Inu)	337:348	inulin (Inu)	337:348	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	9	11	with	use	1357:1359	arg1	WPC					1376:1378	WPC	1376:1378	WPC	1376:1378	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	9	12	theme	oxidative	1510:1518	arg1	stability					1520:1528	higher oxidative stability	1503:1528	higher oxidative stability	1503:1528	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	2	13	located	observed	604:611	arg2	solubility					588:597	solubility	588:597	solubility	588:597	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	2	13	located	observed	604:611	arg2	efficiency					573:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	2	13	located	observed	604:611	arg1	microcapsules					624:636	Fuc-WPC microcapsules	616:636	Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively)	616:678	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	8	14	theme	Sensory	1177:1183	arg1	evaluation					1185:1194	Sensory evaluation	1177:1194	Sensory evaluation of fortified natural yogurt with microcapsules	1177:1241	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	1	15	theme	particle	407:414	arg1	efficiency					461:470	encapsulation efficiency	447:470	encapsulation efficiency	447:470	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	15	theme	particle	407:414	arg1	distribution					421:432	particle size distribution	407:432	particle size distribution	407:432	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	15	theme	particle	407:414	arg1	…					485:485	…	485:485	…	485:485	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	15	theme	particle	407:414	arg1	solubility					473:482	solubility	473:482	solubility	473:482	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	15	theme	particle	407:414	arg1	morphology					435:444	morphology	435:444	morphology	435:444	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	3	16	theme	higher	736:741	arg1	stability					753:761	higher oxidative stability	736:761	higher oxidative stability	736:761	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	1	17	theme	oxidative	489:497	arg1	stability					499:507	oxidative stability	489:507	oxidative stability	489:507	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	18	from	fucoidan	354:361	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	4	19	theme	higher	793:798	arg1	values					800:805	The higher values	789:805	The higher values of PUFA and SFA	789:821	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	1	20	theme	size	416:419	arg1	efficiency					461:470	encapsulation efficiency	447:470	encapsulation efficiency	447:470	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	20	theme	size	416:419	arg1	distribution					421:432	particle size distribution	407:432	particle size distribution	407:432	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	20	theme	size	416:419	arg1	…					485:485	…	485:485	…	485:485	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	20	theme	size	416:419	arg1	solubility					473:482	solubility	473:482	solubility	473:482	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	20	theme	size	416:419	arg1	morphology					435:444	morphology	435:444	morphology	435:444	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	5	21	theme	porous	941:946	arg1	structure					948:956	a more porous structure	934:956	a more porous structure	934:956	The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC.
30361022	1	22	theme	stability	499:507	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	23	theme	freeze-dried	183:194	arg1	microcapsules					196:208	freeze-dried microcapsules	183:208	freeze-dried microcapsules	183:208	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	0	24	theme	WPC-inulin-fucoidan	6:24	arg1	complexes					26:34	WPC-inulin-fucoidan complexes	6:34	WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality	6:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	24	theme	WPC-inulin-fucoidan	6:24	arg1	Characterization					116:131	Characterization	116:131	Characterization	116:131	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	24	theme	WPC-inulin-fucoidan	6:24	arg1	quality					149:155	nutritional quality	137:155	nutritional quality	137:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	25	from	encapsulation	40:52	arg1	emulsion					106:113	W1/O/W2 emulsion	98:113	W1/O/W2 emulsion	98:113	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	4	26	theme	PUFA	810:813	arg1	values					800:805	The higher values	789:805	The higher values of PUFA and SFA	789:821	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	5	27	theme	Fuc-WPC	889:895	arg1	micrographs					915:925	The Fuc-WPC and Inu + Fuc-WPC micrographs	885:925	The Fuc-WPC and Inu + Fuc-WPC micrographs	885:925	The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC.
30361022	2	28	dep	microcapsules	624:636	arg1	30.26 mg/100 g					650:663	30.26 mg/100 g	650:663	30.26 mg/100 g	650:663	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	2	28	dep	microcapsules	624:636	arg1	%					644:644	86.31%	639:644	86.31%	639:644	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	8	29	theme	fishy	1256:1260	arg1	flavor					1262:1267	lower fishy flavor	1250:1267	lower fishy flavor	1250:1267	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	6	30	theme	mean	983:986	arg1	size					997:1000	The mean particle size	979:1000	The mean particle size	979:1000	The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm.
30361022	2	31	theme	encapsulation	559:571	arg1	efficiency					573:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency	552:582	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	9	32	theme	inulin	1364:1369	arg1	use					1357:1359	the use	1353:1359	the use of inulin with WPC as a wall materal	1353:1396	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	1	33	theme	nutritional	510:520	arg1	quality					522:528	nutritional quality	510:528	nutritional quality	510:528	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	8	34	theme	lower	1250:1254	arg1	flavor					1262:1267	lower fishy flavor	1250:1267	lower fishy flavor	1250:1267	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	8	35	theme	yogurt	1217:1222	arg1	evaluation					1185:1194	Sensory evaluation	1177:1194	Sensory evaluation of fortified natural yogurt with microcapsules	1177:1241	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	4	36	located	observed	828:835	arg1	microcapsules					856:868	Inu-WPC Fuc-WPC microcapsules	840:868	Inu-WPC Fuc-WPC microcapsules	840:868	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	4	36	located	observed	828:835	arg2	values					800:805	The higher values	789:805	The higher values of PUFA and SFA	789:821	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	7	37	theme	released	1098:1105	arg1	oil					1107:1109	the released oil	1094:1109	the released oil	1094:1109	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	5	38	theme	Inu + Fuc-WPC	901:913	arg1	micrographs					915:925	The Fuc-WPC and Inu + Fuc-WPC micrographs	885:925	The Fuc-WPC and Inu + Fuc-WPC micrographs	885:925	The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC.
30361022	1	39	theme	quality	522:528	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	40	theme	fish	221:224	arg1	FPH					247:249	FPH	247:249	FPH	247:249	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	40	theme	fish	221:224	arg1	hydrolysate					234:244	fish protein hydrolysate	221:244	fish protein hydrolysate (FPH)	221:250	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	3	41	from	combination	685:695	arg1	material					720:727	the wall material	711:727	the wall material	711:727	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	9	42	with	use	1468:1470	arg1	WPC					1489:1491	WPC	1489:1491	WPC	1489:1491	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	1	43	contain	containing	210:219	arg2	FO					266:267	FO	266:267	FO	266:267	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	43	contain	containing	210:219	arg2	hydrolysate					234:244	fish protein hydrolysate	221:244	fish protein hydrolysate (FPH)	221:250	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	43	contain	containing	210:219	arg2	oil					261:263	fish oil	256:263	fish oil (FO)	256:268	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	43	contain	containing	210:219	arg2	FPH					247:249	FPH	247:249	FPH	247:249	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	43	contain	containing	210:219	arg1	emulsions					169:177	The double emulsions	158:177	The double emulsions	158:177	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	8	44	with	yogurt	1217:1222	arg1	microcapsules					1229:1241	microcapsules	1229:1241	microcapsules	1229:1241	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	1	45	theme	protein	226:232	arg1	FPH					247:249	FPH	247:249	FPH	247:249	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	45	theme	protein	226:232	arg1	hydrolysate					234:244	fish protein hydrolysate	221:244	fish protein hydrolysate (FPH)	221:250	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	7	46	theme	fatty	1119:1123	arg1	composition					1130:1140	the fatty acid composition	1115:1140	the fatty acid composition	1115:1140	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	3	47	theme	Fuc-WPC	700:706	arg1	combination					685:695	The combination	681:695	The combination of Fuc-WPC in the wall material	681:727	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	9	48	theme	fucoidan	1475:1482	arg1	use					1468:1470	the use	1464:1470	the use of fucoidan with WPC	1464:1491	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	1	49	theme	in	534:535	arg1	release					543:549	in vitro release	534:549	in vitro release	534:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	7	50	theme	acid	1125:1128	arg1	composition					1130:1140	the fatty acid composition	1115:1140	the fatty acid composition	1115:1140	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	0	51	theme	nutritional	137:147	arg1	complexes					26:34	WPC-inulin-fucoidan complexes	6:34	WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality	6:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	51	theme	nutritional	137:147	arg1	quality					149:155	nutritional quality	137:155	nutritional quality	137:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	3	52	theme	wall	715:718	arg1	material					720:727	the wall material	711:727	the wall material	711:727	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	8	53	theme	Inu-WPC	1272:1278	arg1	samples					1280:1286	Inu-WPC samples	1272:1286	Inu-WPC samples	1272:1286	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	0	54	theme	protein	62:68	arg1	hydrolysate					70:80	fish protein hydrolysate	57:80	fish protein hydrolysate	57:80	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	1	55	theme	whey	301:304	arg1	WPC					327:329	WPC	327:329	WPC	327:329	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	55	theme	whey	301:304	arg1	concentrate					314:324	whey protein concentrate	301:324	whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	301:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	4	56	theme	Inu-WPC	840:846	arg1	microcapsules					856:868	Inu-WPC Fuc-WPC microcapsules	840:868	Inu-WPC Fuc-WPC microcapsules	840:868	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	1	57	theme	encapsulation	447:459	arg1	efficiency					461:470	encapsulation efficiency	447:470	encapsulation efficiency	447:470	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	57	theme	encapsulation	447:459	arg1	distribution					421:432	particle size distribution	407:432	particle size distribution	407:432	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	0	58	theme	fish	57:60	arg1	hydrolysate					70:80	fish protein hydrolysate	57:80	fish protein hydrolysate	57:80	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	1	59	theme	protein	306:312	arg1	WPC					327:329	WPC	327:329	WPC	327:329	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	59	theme	protein	306:312	arg1	concentrate					314:324	whey protein concentrate	301:324	whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	301:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	8	60	theme	fortified	1199:1207	arg1	yogurt					1217:1222	fortified natural yogurt	1199:1222	fortified natural yogurt with microcapsules	1199:1241	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	8	61	theme	natural	1209:1215	arg1	yogurt					1217:1222	fortified natural yogurt	1199:1222	fortified natural yogurt with microcapsules	1199:1241	Sensory evaluation of fortified natural yogurt with microcapsules showed lower fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC.
30361022	7	62	located	observed	1082:1089	arg1	composition					1130:1140	the fatty acid composition	1115:1140	the fatty acid composition	1115:1140	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	7	62	located	observed	1082:1089	arg2	differences					1065:1075	No significant differences	1050:1075	No significant differences	1050:1075	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	7	62	located	observed	1082:1089	arg1	oil					1107:1109	the released oil	1094:1109	the released oil	1094:1109	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	1	63	theme	concentrate	314:324	arg1	complexs					289:296	complexs	289:296	complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	289:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	9	64	theme	good	1410:1413	arg1	characteristics					1415:1429	good characteristics	1410:1429	good characteristics	1410:1429	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	1	65	with	concentrate	314:324	arg1	Fuc					364:366	Fuc	364:366	Fuc	364:366	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	65	with	concentrate	314:324	arg1	Inu					345:347	inulin (Inu)	337:348	inulin (Inu)	337:348	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	65	with	concentrate	314:324	arg1	fucoidan					354:361	fucoidan	354:361	fucoidan	354:361	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	66	theme	release	543:549	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	6	67	theme	particle	988:995	arg1	size					997:1000	The mean particle size	979:1000	The mean particle size	979:1000	The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm.
30361022	4	68	theme	Fuc-WPC	848:854	arg1	microcapsules					856:868	Inu-WPC Fuc-WPC microcapsules	840:868	Inu-WPC Fuc-WPC microcapsules	840:868	The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively.
30361022	0	69	dep	complexes	26:34	arg1	complexes					26:34	WPC-inulin-fucoidan complexes	6:34	WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality	6:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	69	dep	complexes	26:34	arg1	Characterization					116:131	Characterization	116:131	Characterization	116:131	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	0	69	dep	complexes	26:34	arg1	quality					149:155	nutritional quality	137:155	nutritional quality	137:155	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	2	70	theme	Fuc-WPC	616:622	arg1	microcapsules					624:636	Fuc-WPC microcapsules	616:636	Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively)	616:678	Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively).
30361022	9	71	theme	higher	1503:1508	arg1	stability					1520:1528	higher oxidative stability	1503:1528	higher oxidative stability	1503:1528	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	3	72	theme	other	768:772	arg1	material					779:786	other wall material	768:786	other wall material	768:786	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	1	73	dep	in	534:535	arg1	vitro					537:541	vitro	537:541	vitro	537:541	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	7	74	theme	gastrointestinal	1149:1164	arg1	digestion					1166:1174	gastrointestinal digestion	1149:1174	gastrointestinal digestion	1149:1174	No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion.
30361022	1	75	dep	characteristics	390:404	arg1	efficiency					461:470	encapsulation efficiency	447:470	encapsulation efficiency	447:470	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	75	dep	characteristics	390:404	arg1	distribution					421:432	particle size distribution	407:432	particle size distribution	407:432	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	75	dep	characteristics	390:404	arg1	…					485:485	…	485:485	…	485:485	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	75	dep	characteristics	390:404	arg1	solubility					473:482	solubility	473:482	solubility	473:482	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	75	dep	characteristics	390:404	arg1	morphology					435:444	morphology	435:444	morphology	435:444	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	3	76	theme	oxidative	743:751	arg1	stability					753:761	higher oxidative stability	736:761	higher oxidative stability	736:761	The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material.
30361022	0	77	theme	hydrolysate	70:80	arg1	encapsulation					40:52	encapsulation	40:52	encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion	40:113	Using WPC-inulin-fucoidan complexes for encapsulation of fish protein hydrolysate and fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
30361022	9	78	theme	wall	1385:1388	arg1	materal					1390:1396	a wall materal	1383:1396	a wall materal	1383:1396	In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
30361022	1	79	from	Inu	345:347	arg1	terms					372:376	terms	372:376	terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release	372:549	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	80	theme	fish	256:259	arg1	FO					266:267	FO	266:267	FO	266:267	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
30361022	1	80	theme	fish	256:259	arg1	oil					261:263	fish oil	256:263	fish oil (FO)	256:268	The double emulsions and freeze-dried microcapsules containing fish protein hydrolysate (FPH) and fish oil (FO) were stabilized by complexs of whey protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release.
31733248	4	0	theme	MB	853:854	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	5	1	attach	removal	868:874	arg3	system					898:903	the binary system	887:903	the binary system	887:903	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	5	1	attach	removal	868:874	arg2	dyes					879:882	dyes	879:882	dyes	879:882	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	1	2	theme	powdered	176:183	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	2	theme	powdered	176:183	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	2	theme	powdered	176:183	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	0	3	theme	aqueous	68:74	arg1	mediums					76:82	aqueous mediums	68:82	aqueous mediums onto cellulose-based	68:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	3	4	theme	MB	628:629	arg1	adsorption					614:623	the adsorption	610:623	the adsorption of MB and CV onto adsorbents	610:652	Kinetics modeling shows that the adsorption of MB and CV onto adsorbents was well described by pseudo-first-order.
31733248	5	5	from	removal	868:874	arg1	system					898:903	the binary system	887:903	the binary system	887:903	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	6	6	theme	high	1039:1042	arg1	capacities					1055:1064	very high adsorption capacities	1034:1064	very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems	1034:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	1	7	theme	binary	378:383	arg1	adsorption					385:394	the single and binary adsorption	363:394	the single and binary adsorption of MB and CV dyes	363:412	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	2	8	theme	dyes	504:507	arg1	solution					509:516	initial dyes solution	496:516	initial dyes solution	496:516	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	9	dep	investigated	469:480	arg1	25-300 mg·L-1					565:577	25-300 mg·L-1	565:577	25-300 mg·L-1	565:577	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	9	dep	investigated	469:480	arg1	concentration					550:562	initial dyes concentration	537:562	initial dyes concentration (25-300 mg·L-1)	537:578	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	9	dep	investigated	469:480	arg1	time					527:530	contact time	519:530	contact time	519:530	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	9	dep	investigated	469:480	arg1	pH					483:484	pH (3-11)	483:491	pH (3-11) of initial dyes solution	483:516	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	6	10	theme	single	1127:1132	arg1	systems					1144:1150	single or binary systems	1127:1150	single or binary systems	1127:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	2	11	theme	initial	496:502	arg1	solution					509:516	initial dyes solution	496:516	initial dyes solution	496:516	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	6	12	theme	adsorption	1044:1053	arg1	capacities					1055:1064	very high adsorption capacities	1034:1064	very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems	1034:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	0	13	theme	citrus	114:119	arg1	composite					144:152	citrus peels/calcium alginate composite	114:152	citrus peels/calcium alginate composite	114:152	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	2	14	theme	contact	519:525	arg1	time					527:530	contact time	519:530	contact time	519:530	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	6	15	theme	treatment	1114:1122	arg1	field					1099:1103	the field	1095:1103	the field of water treatment in single or binary systems	1095:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	1	16	theme	encapsulated	189:200	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	16	theme	encapsulated	189:200	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	16	theme	encapsulated	189:200	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	17	theme	single	367:372	arg1	adsorption					385:394	the single and binary adsorption	363:394	the single and binary adsorption of MB and CV dyes	363:412	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	4	18	theme	homogeneous	813:823	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	4	19	from	capacities	717:726	arg1	CPAA-A					731:736	CPAA-A	731:736	CPAA-A	731:736	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	6	20	theme	water	1108:1112	arg1	treatment					1114:1122	water treatment	1108:1122	water treatment	1108:1122	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	1	21	theme	cellulose-based	202:216	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	21	theme	cellulose-based	202:216	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	21	theme	cellulose-based	202:216	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	0	22	from	studies	34:40	arg1	mediums					76:82	aqueous mediums	68:82	aqueous mediums onto cellulose-based	68:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	4	23	theme	MB	703:704	arg1	capacities					717:726	MB adsorption capacities	703:726	MB adsorption capacities	703:726	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	1	24	theme	modified	218:225	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	24	theme	modified	218:225	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	24	theme	modified	218:225	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	0	25	theme	Single	0:5	arg1	studies					34:40	Single and competitive adsorption studies	0:40	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based	0:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	4	26	theme	monolayer	829:837	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	1	27	theme	citrus	227:232	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	27	theme	citrus	227:232	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	27	theme	citrus	227:232	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	0	28	theme	adsorption	23:32	arg1	studies					34:40	Single and competitive adsorption studies	0:40	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based	0:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	6	29	theme	binary	1137:1142	arg1	systems					1144:1150	single or binary systems	1127:1150	single or binary systems	1127:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	0	30	theme	alginate	135:142	arg1	composite					144:152	citrus peels/calcium alginate composite	114:152	citrus peels/calcium alginate composite	114:152	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	5	31	theme	dyes	995:998	arg1	presence					979:986	the simultaneous presence	962:986	the simultaneous presence of the dyes in solution	962:1010	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	5	32	from	solution	1003:1010	arg1	presence					979:986	the simultaneous presence	962:986	the simultaneous presence of the dyes in solution	962:1010	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	5	33	from	dyes	995:998	arg1	solution					1003:1010	solution	1003:1010	solution	1003:1010	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	0	34	theme	competitive	11:21	arg1	studies					34:40	Single and competitive adsorption studies	0:40	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based	0:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	0	35	theme	peels/calcium	121:133	arg1	composite					144:152	citrus peels/calcium alginate composite	114:152	citrus peels/calcium alginate composite	114:152	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	0	36	from	mediums	76:82	arg1	dyes					58:61	two cationic dyes	45:61	two cationic dyes from aqueous mediums onto cellulose-based	45:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	0	36	from	mediums	76:82	arg1	studies					34:40	Single and competitive adsorption studies	0:40	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based	0:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	4	37	theme	adsorption	706:715	arg1	capacities					717:726	MB adsorption capacities	703:726	MB adsorption capacities	703:726	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	5	38	from	presence	979:986	arg1	solution					1003:1010	solution	1003:1010	solution	1003:1010	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	5	39	theme	simultaneous	966:977	arg1	presence					979:986	the simultaneous presence	962:986	the simultaneous presence of the dyes in solution	962:1010	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	4	40	theme	Langmuir	790:797	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	6	41	from	field	1099:1103	arg1	systems					1144:1150	single or binary systems	1127:1150	single or binary systems	1127:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	6	41	from	field	1099:1103	arg1	useful					1085:1090	useful	1085:1090	useful	1085:1090	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	2	42	theme	solution	509:516	arg1	25-300 mg·L-1					565:577	25-300 mg·L-1	565:577	25-300 mg·L-1	565:577	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	42	theme	solution	509:516	arg1	concentration					550:562	initial dyes concentration	537:562	initial dyes concentration (25-300 mg·L-1)	537:578	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	42	theme	solution	509:516	arg1	time					527:530	contact time	519:530	contact time	519:530	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	42	theme	solution	509:516	arg1	pH					483:484	pH (3-11)	483:491	pH (3-11) of initial dyes solution	483:516	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	0	43	theme	cationic	49:56	arg1	dyes					58:61	two cationic dyes	45:61	two cationic dyes from aqueous mediums onto cellulose-based	45:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	2	44	theme	dyes	545:548	arg1	25-300 mg·L-1					565:577	25-300 mg·L-1	565:577	25-300 mg·L-1	565:577	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	44	theme	dyes	545:548	arg1	concentration					550:562	initial dyes concentration	537:562	initial dyes concentration (25-300 mg·L-1)	537:578	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	1	45	theme	MB	399:400	arg1	adsorption					385:394	the single and binary adsorption	363:394	the single and binary adsorption of MB and CV dyes	363:412	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	4	46	theme	showing	805:811	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	5	47	theme	dyes	879:882	arg1	removal					868:874	The removal	864:874	The removal of dyes in the binary system	864:903	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	2	48	theme	adsorption	443:452	arg1	phenomena					454:462	adsorption phenomena	443:462	adsorption phenomena	443:462	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	49	theme	initial	537:543	arg1	25-300 mg·L-1					565:577	25-300 mg·L-1	565:577	25-300 mg·L-1	565:577	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	2	49	theme	initial	537:543	arg1	concentration					550:562	initial dyes concentration	537:562	initial dyes concentration (25-300 mg·L-1)	537:578	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	3	50	theme	CV	635:636	arg1	adsorption					614:623	the adsorption	610:623	the adsorption of MB and CV onto adsorbents	610:652	Kinetics modeling shows that the adsorption of MB and CV onto adsorbents was well described by pseudo-first-order.
31733248	4	51	theme	model	799:803	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	6	52	from	useful	1085:1090	arg1	field					1099:1103	the field	1095:1103	the field of water treatment in single or binary systems	1095:1150	CPAA-A and CPAA show very high adsorption capacities proving to be very useful in the field of water treatment in single or binary systems.
31733248	4	53	theme	CV	860:861	arg1	adsorption					839:848	Langmuir model showing homogeneous and monolayer adsorption	790:848	Langmuir model showing homogeneous and monolayer adsorption of MB and CV	790:861	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	4	54	from	CV	696:697	arg1	CPAA-A					731:736	CPAA-A	731:736	CPAA-A	731:736	CV and MB adsorption capacities on CPAA-A were respectively 881.36 and 923.07 mg·g-1 found by Langmuir model showing homogeneous and monolayer adsorption of MB and CV.
31733248	3	55	theme	Kinetics	581:588	arg1	modeling					590:597	Kinetics modeling	581:597	Kinetics modeling	581:597	Kinetics modeling shows that the adsorption of MB and CV onto adsorbents was well described by pseudo-first-order.
31733248	1	56	theme	CV	406:407	arg1	dyes					409:412	CV dyes	406:412	CV dyes	406:412	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	57	theme	dyes	409:412	arg1	adsorption					385:394	the single and binary adsorption	363:394	the single and binary adsorption of MB and CV dyes	363:412	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	5	58	theme	binary	891:896	arg1	system					898:903	the binary system	887:903	the binary system	887:903	The removal of dyes in the binary system shows that adsorption was slightly or nether affected by the simultaneous presence of the dyes in solution.
31733248	1	59	dep	peels	234:238	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	59	dep	peels	234:238	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	59	dep	peels	234:238	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	60	theme	Acetic	155:160	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	60	theme	Acetic	155:160	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	60	theme	Acetic	155:160	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	0	61	theme	dyes	58:61	arg1	studies					34:40	Single and competitive adsorption studies	0:40	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based	0:103	Single and competitive adsorption studies of two cationic dyes from aqueous mediums onto cellulose-based modified citrus peels/calcium alginate composite.
31733248	1	62	theme	Boehm	332:336	arg1	FTIR					301:304	FTIR	301:304	FTIR	301:304	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	62	theme	Boehm	332:336	arg1	analysis					338:345	Boehm analysis	332:345	Boehm analysis	332:345	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	2	63	theme	Many	415:418	arg1	parameters					420:429	Many parameters	415:429	Many parameters that affect adsorption phenomena	415:462	Many parameters that affect adsorption phenomena were investigated: pH (3-11) of initial dyes solution, contact time, and initial dyes concentration (25-300 mg·L-1).
31733248	1	64	theme	acid-modified	162:174	arg1	CPAA					240:243	CPAA	240:243	CPAA	240:243	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	64	theme	acid-modified	162:174	arg1	peels					234:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels	155:238	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A	155:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31733248	1	64	theme	acid-modified	162:174	arg1	CPAA-A					249:254	CPAA-A	249:254	CPAA-A	249:254	Acetic acid-modified powdered and encapsulated cellulose-based modified citrus peels CPAA and CPAA-A were easily prepared and characterized using FTIR, DRX, BET, SEM, pHPZC and Boehm analysis, to investigate the single and binary adsorption of MB and CV dyes.
31751731	2	0	theme	observed	420:427	arg1	data					429:432	The observed data	416:432	The observed data	416:432	The observed data was evaluated by statistically and a mathematical model (polynomial) was developed to predict the polysaccharide yield.
31751731	2	1	theme	polysaccharide	532:545	arg1	yield					547:551	the polysaccharide yield	528:551	the polysaccharide yield	528:551	The observed data was evaluated by statistically and a mathematical model (polynomial) was developed to predict the polysaccharide yield.
31751731	1	2	from	seeds	183:187	arg1	polysaccharide					151:164	polysaccharide	151:164	polysaccharide from jamun fruit seeds (waste)	151:195	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	0	3	theme	fruit	86:90	arg1	seeds					92:96	waste jamun fruit seeds	74:96	waste jamun fruit seeds	74:96	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	1	4	theme	composite	367:375	arg1	SL					337:338	SL	337:338	SL	337:338	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	4	theme	composite	367:375	arg1	rotatable					377:385	liquid (SL) ratio) five-level central composite rotatable	329:385	liquid (SL) ratio) five-level central composite rotatable	329:385	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	5	theme	jamun	171:175	arg1	waste					190:194	waste	190:194	waste	190:194	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	5	theme	jamun	171:175	arg1	seeds					183:187	jamun fruit seeds	171:187	jamun fruit seeds (waste)	171:195	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	6	theme	liquid	329:334	arg1	SL					337:338	SL	337:338	SL	337:338	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	6	theme	liquid	329:334	arg1	rotatable					377:385	liquid (SL) ratio) five-level central composite rotatable	329:385	liquid (SL) ratio) five-level central composite rotatable	329:385	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	3	7	theme	attained	761:768	arg1	condition					778:786	attained optimal condition	761:786	attained optimal condition	761:786	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	8	theme	1:15 g/ml	702:710	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	8	theme	1:15 g/ml	702:710	arg1	pH					660:661	pH	660:661	pH of 3.2	660:668	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	8	theme	1:15 g/ml	702:710	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	8	theme	1:15 g/ml	702:710	arg1	MWT					671:673	MWT	671:673	MWT of 3.1 min	671:684	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	4	9	theme	polysaccharide	844:857	arg1	yield					835:839	The experimental yield	818:839	The experimental yield of polysaccharide (4.71 ± 0.02%)	818:872	The experimental yield of polysaccharide (4.71 ± 0.02%) was matched with predicted value (4.72%).
31751731	3	10	theme	w	657:657	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	10	theme	w	657:657	arg1	pH					660:661	pH	660:661	pH of 3.2	660:668	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	10	theme	w	657:657	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	10	theme	w	657:657	arg1	MWT					671:673	MWT	671:673	MWT of 3.1 min	671:684	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	11	theme	experimental	387:398	arg1	CCRED					408:412	CCRED	408:412	CCRED	408:412	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	11	theme	experimental	387:398	arg1	design					400:405	solid to liquid (SL) ratio) five-level central composite rotatable experimental design	320:405	solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	320:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	12	theme	fruit	177:181	arg1	waste					190:194	waste	190:194	waste	190:194	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	12	theme	fruit	177:181	arg1	seeds					183:187	jamun fruit seeds	171:187	jamun fruit seeds (waste)	171:195	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	13	theme	solid	320:324	arg1	CCRED					408:412	CCRED	408:412	CCRED	408:412	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	13	theme	solid	320:324	arg1	design					400:405	solid to liquid (SL) ratio) five-level central composite rotatable experimental design	320:405	solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	320:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	7	14	theme	plant	1239:1243	arg1	sample					1245:1250	plant sample	1239:1250	plant sample	1239:1250	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	0	15	theme	assisted	10:17	arg1	extraction					19:28	Microwave assisted extraction	0:28	Microwave assisted extraction	0:28	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	3	16	dep	condition	623:631	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	16	dep	condition	623:631	arg1	pH					660:661	pH	660:661	pH of 3.2	660:668	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	16	dep	condition	623:631	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	16	dep	condition	623:631	arg1	MWT					671:673	MWT	671:673	MWT of 3.1 min	671:684	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	7	17	theme	raw	1221:1223	arg1	morphology					1207:1216	the morphology	1203:1216	the morphology of raw	1203:1223	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	0	18	theme	Microwave	0:8	arg1	extraction					19:28	Microwave assisted extraction	0:28	Microwave assisted extraction	0:28	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	3	19	theme	3.2	666:668	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	19	theme	3.2	666:668	arg1	pH					660:661	pH	660:661	pH of 3.2	660:668	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	19	theme	3.2	666:668	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	19	theme	3.2	666:668	arg1	MWT					671:673	MWT	671:673	MWT of 3.1 min	671:684	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	5	20	theme	polysaccharide	951:964	arg1	properties					933:942	Physico-chemical properties	916:942	Physico-chemical properties of the polysaccharide extracted at optimal condition	916:995	Physico-chemical properties of the polysaccharide extracted at optimal condition was investigated.
31751731	3	21	theme	ideal	609:613	arg1	condition					623:631	the ideal optimal condition	605:631	the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml)	605:711	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	22	theme	microwave	278:286	arg1	power					288:292	microwave power	278:292	microwave power	278:292	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	22	theme	microwave	278:286	arg1	four-factor					265:275	four-factor	265:275	four-factor (microwave power (MWP)	265:298	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	4	23	theme	experimental	822:833	arg1	yield					835:839	The experimental yield	818:839	The experimental yield of polysaccharide (4.71 ± 0.02%)	818:872	The experimental yield of polysaccharide (4.71 ± 0.02%) was matched with predicted value (4.72%).
31751731	7	24	used	utilized	1184:1191	arg2	Microscopy					1158:1167	Scanning Electron Microscopy	1140:1167	Scanning Electron Microscopy (SEM)	1140:1173	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	7	24	used	utilized	1184:1191	arg2	SEM					1170:1172	SEM	1170:1172	SEM	1170:1172	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	0	25	from	seeds	92:96	arg1	extraction					19:28	Microwave assisted extraction	0:28	Microwave assisted extraction	0:28	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	0	25	from	seeds	92:96	arg1	characterization					34:49	characterization	34:49	characterization	34:49	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	3	26	used	used	590:593	arg2	technique					577:585	Numerical optimization technique	554:585	Numerical optimization technique	554:585	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	2	27	theme	mathematical	471:482	arg1	model					484:488	a mathematical model	469:488	a mathematical model (polynomial)	469:501	The observed data was evaluated by statistically and a mathematical model (polynomial) was developed to predict the polysaccharide yield.
31751731	2	27	theme	mathematical	471:482	arg1	polynomial					491:500	polynomial	491:500	polynomial	491:500	The observed data was evaluated by statistically and a mathematical model (polynomial) was developed to predict the polysaccharide yield.
31751731	1	28	theme	microwave	200:208	arg1	technique					249:257	microwave assisted solid-liquid extraction (MAE) technique	200:257	microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	200:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	4	29	theme	predicted	891:899	arg1	value					901:905	predicted value	891:905	predicted value (4.72%)	891:913	The experimental yield of polysaccharide (4.71 ± 0.02%) was matched with predicted value (4.72%).
31751731	4	29	theme	predicted	891:899	arg1	%					912:912	4.72%	908:912	4.72%	908:912	The experimental yield of polysaccharide (4.71 ± 0.02%) was matched with predicted value (4.72%).
31751731	5	30	theme	optimal	979:985	arg1	condition					987:995	optimal condition	979:995	optimal condition	979:995	Physico-chemical properties of the polysaccharide extracted at optimal condition was investigated.
31751731	1	31	theme	assisted	210:217	arg1	technique					249:257	microwave assisted solid-liquid extraction (MAE) technique	200:257	microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	200:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	3	32	theme	min	682:684	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	32	theme	min	682:684	arg1	pH					660:661	pH	660:661	pH of 3.2	660:668	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	32	theme	min	682:684	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	32	theme	min	682:684	arg1	MWT					671:673	MWT	671:673	MWT of 3.1 min	671:684	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	7	33	theme	Scanning	1140:1147	arg1	SEM					1170:1172	SEM	1170:1172	SEM	1170:1172	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	7	33	theme	Scanning	1140:1147	arg1	Microscopy					1158:1167	Scanning Electron Microscopy	1140:1167	Scanning Electron Microscopy (SEM)	1140:1173	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	7	34	theme	Electron	1149:1156	arg1	SEM					1170:1172	SEM	1170:1172	SEM	1170:1172	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	7	34	theme	Electron	1149:1156	arg1	Microscopy					1158:1167	Scanning Electron Microscopy	1140:1167	Scanning Electron Microscopy (SEM)	1140:1173	Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.
31751731	1	35	theme	current	116:122	arg1	work					124:127	current work	116:127	current work	116:127	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	0	36	theme	polysaccharide	54:67	arg1	extraction					19:28	Microwave assisted extraction	0:28	Microwave assisted extraction	0:28	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	0	36	theme	polysaccharide	54:67	arg1	characterization					34:49	characterization	34:49	characterization	34:49	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	3	37	theme	microwave	634:642	arg1	power					644:648	microwave power	634:648	microwave power of 515 w	634:657	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	38	theme	ratio	341:345	arg1	SL					337:338	SL	337:338	SL	337:338	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	38	theme	ratio	341:345	arg1	rotatable					377:385	liquid (SL) ratio) five-level central composite rotatable	329:385	liquid (SL) ratio) five-level central composite rotatable	329:385	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	5	39	theme	Physico-chemical	916:931	arg1	properties					933:942	Physico-chemical properties	916:942	Physico-chemical properties of the polysaccharide extracted at optimal condition	916:995	Physico-chemical properties of the polysaccharide extracted at optimal condition was investigated.
31751731	1	40	theme	work	124:127	arg1	intention					103:111	The intention	99:111	The intention of current work	99:127	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	6	41	dep	Spectroscopy	1042:1053	arg1	carried					1078:1084	carried	1078:1084	were carried out in this study to characterize the polysaccharide	1073:1137	Fourier Transform Infrared Spectroscopy (FTIR) evaluation were carried out in this study to characterize the polysaccharide.
31751731	3	42	theme	optimal	615:621	arg1	condition					623:631	the ideal optimal condition	605:631	the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml)	605:711	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	43	theme	Numerical	554:562	arg1	technique					577:585	Numerical optimization technique	554:585	Numerical optimization technique	554:585	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	6	44	theme	Infrared	1033:1040	arg1	FTIR					1056:1059	FTIR	1056:1059	FTIR	1056:1059	Fourier Transform Infrared Spectroscopy (FTIR) evaluation were carried out in this study to characterize the polysaccharide.
31751731	6	44	theme	Infrared	1033:1040	arg1	Spectroscopy					1042:1053	Infrared Spectroscopy	1033:1053	Infrared Spectroscopy (FTIR) evaluation were carried out in this study to characterize the polysaccharide	1033:1137	Fourier Transform Infrared Spectroscopy (FTIR) evaluation were carried out in this study to characterize the polysaccharide.
31751731	3	45	theme	polysaccharide	742:755	arg1	yield					733:737	maximal yield	725:737	maximal yield of polysaccharide	725:755	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	46	theme	solid-liquid	219:230	arg1	technique					249:257	microwave assisted solid-liquid extraction (MAE) technique	200:257	microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	200:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	47	theme	five-level	348:357	arg1	SL					337:338	SL	337:338	SL	337:338	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	47	theme	five-level	348:357	arg1	rotatable					377:385	liquid (SL) ratio) five-level central composite rotatable	329:385	liquid (SL) ratio) five-level central composite rotatable	329:385	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	3	48	theme	optimization	564:575	arg1	technique					577:585	Numerical optimization technique	554:585	Numerical optimization technique	554:585	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	49	theme	maximal	725:731	arg1	yield					733:737	maximal yield	725:737	maximal yield of polysaccharide	725:755	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	50	theme	extraction	232:241	arg1	technique					249:257	microwave assisted solid-liquid extraction (MAE) technique	200:257	microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	200:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	0	51	theme	jamun	80:84	arg1	seeds					92:96	waste jamun fruit seeds	74:96	waste jamun fruit seeds	74:96	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	1	52	theme	central	359:365	arg1	SL					337:338	SL	337:338	SL	337:338	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	1	52	theme	central	359:365	arg1	rotatable					377:385	liquid (SL) ratio) five-level central composite rotatable	329:385	liquid (SL) ratio) five-level central composite rotatable	329:385	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	0	53	theme	waste	74:78	arg1	seeds					92:96	waste jamun fruit seeds	74:96	waste jamun fruit seeds	74:96	Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds.
31751731	1	54	dep	power	288:292	arg1	MWP					295:297	MWP	295:297	MWP	295:297	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31751731	3	55	theme	SL	690:691	arg1	ratio					693:697	SL ratio	690:697	SL ratio of 1:15 g/ml	690:710	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	3	56	theme	optimal	770:776	arg1	condition					778:786	attained optimal condition	761:786	attained optimal condition	761:786	Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated.
31751731	1	57	theme	MAE	244:246	arg1	technique					249:257	microwave assisted solid-liquid extraction (MAE) technique	200:257	microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED)	200:413	The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED).
31000229	4	0	dep	mono-	684:688	arg1	The					680:682	The	680:682	The	680:682	The mono- and two-dimensional electrophoretic profiles of Prosopis spp.
31000229	2	1	from	algarrobo	277:285	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	6	2	theme	germ	1014:1017	arg1	flour					1019:1023	carob germ flour	1008:1023	carob germ flour	1008:1023	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	3	3	theme	proteomics	642:651	arg1	methods					671:677	proteomics and complementary methods	642:677	methods	671:677	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	3	4	theme	ruscifolia	608:617	arg1	counterparts					619:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	3	5	theme	nigra	595:599	arg1	counterparts					619:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	6	6	theme	carob	1008:1012	arg1	flour					1019:1023	carob germ flour	1008:1023	carob germ flour	1008:1023	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	8	7	theme	nutritional	1169:1179	arg1	value					1181:1185	a more adequate nutritional value	1153:1185	a more adequate nutritional value than C. siliqua	1153:1201	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	9	8	dep	in	1336:1337	arg1	vitro					1339:1343	vitro	1339:1343	vitro	1339:1343	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	9	theme	algarrobo	1264:1272	arg1	flour					1279:1283	algarrobo germ flour	1264:1283	algarrobo germ flour	1264:1283	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	6	10	theme	ATR-FTIR	898:905	arg1	spectroscopy					908:919	infrared (ATR-FTIR) spectroscopy	888:919	infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua	888:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	3	11	theme	germ	537:540	arg1	content					550:556	the germ protein content	533:556	the germ protein content of carob	533:565	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	0	12	theme	Prosopis	90:97	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis	0:97	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	2	13	theme	nutrient	144:151	arg1	composition					153:163	exclusive nutrient composition	134:163	exclusive nutrient composition	134:163	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	4	14	theme	Prosopis	738:745	arg1	profiles					726:733	two-dimensional electrophoretic profiles	694:733	two-dimensional electrophoretic profiles	694:733	The mono- and two-dimensional electrophoretic profiles of Prosopis spp.
31000229	4	14	theme	Prosopis	738:745	arg1	mono-					684:688	mono-	684:688	mono-	684:688	The mono- and two-dimensional electrophoretic profiles of Prosopis spp.
31000229	5	15	theme	significant	816:826	arg1	differences					828:838	significant differences	816:838	significant differences	816:838	were very similar among one another, while C. siliqua exhibited significant differences.
31000229	2	16	theme	exclusive	134:142	arg1	composition					153:163	exclusive nutrient composition	134:163	exclusive nutrient composition	134:163	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	4	17	theme	electrophoretic	710:724	arg1	profiles					726:733	two-dimensional electrophoretic profiles	694:733	two-dimensional electrophoretic profiles	694:733	The mono- and two-dimensional electrophoretic profiles of Prosopis spp.
31000229	6	18	theme	germ	1057:1060	arg1	flour					1062:1066	Prosopis germ flour	1048:1066	Prosopis germ flour	1048:1066	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	8	19	theme	essential	1216:1224	arg1	acid					1232:1235	essential amino acid	1216:1235	essential amino acid complement	1216:1246	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	6	20	theme	infrared	888:895	arg1	spectroscopy					908:919	infrared (ATR-FTIR) spectroscopy	888:919	infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua	888:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	5	21	dep	one	776:778	arg1	another					780:786	another	780:786	another	780:786	were very similar among one another, while C. siliqua exhibited significant differences.
31000229	2	22	from	vinal	325:329	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	0	23	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis	0:97	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	2	24	from	carob	229:233	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	8	25	theme	acid	1232:1235	arg1	complement					1237:1246	essential amino acid complement	1216:1246	essential amino acid complement	1216:1246	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	6	26	theme	food	1083:1086	arg1	applications					1102:1113	baking and food technological applications	1072:1113	baking and food technological applications	1072:1113	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	3	27	theme	protein	542:548	arg1	content					550:556	the germ protein content	533:556	the germ protein content of carob	533:565	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	6	28	theme	technological	1088:1100	arg1	applications					1102:1113	baking and food technological applications	1072:1113	baking and food technological applications	1072:1113	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	6	29	theme	baking	1072:1077	arg1	applications					1102:1113	baking and food technological applications	1072:1113	baking and food technological applications	1072:1113	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	2	30	theme	health-promoting	400:415	arg1	ingredient					417:426	health-promoting ingredient	400:426	health-promoting ingredient	400:426	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	8	31	theme	complement	1237:1246	arg1	terms					1207:1211	terms	1207:1211	terms of essential amino acid complement	1207:1246	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	2	32	theme	Prosopis	288:295	arg1	alba					297:300	Prosopis alba	288:300	Prosopis alba	288:300	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	6	33	theme	total	852:856	arg1	reflectance-Fourier					858:876	Attenuated total reflectance-Fourier	841:876	Attenuated total reflectance-Fourier	841:876	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	0	34	theme	protein	24:30	arg1	composition					32:42	protein composition	24:42	protein composition	24:42	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	9	35	theme	in	1336:1337	arg1	digestion					1372:1380	in vitro simulated gastrointestinal digestion	1336:1380	in vitro simulated gastrointestinal digestion	1336:1380	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	36	theme	minor	1435:1439	arg1	proportions					1441:1451	only minor proportions	1430:1451	only minor proportions of low molecular weight peptides	1430:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	6	37	theme	Prosopis	1048:1055	arg1	flour					1062:1066	Prosopis germ flour	1048:1066	Prosopis germ flour	1048:1066	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	2	38	theme	nutritional	378:388	arg1	value					390:394	a high nutritional value	371:394	a high nutritional value	371:394	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	38	theme	nutritional	378:388	arg1	potential					358:366	potential	358:366	potential	358:366	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	4	39	theme	two-dimensional	694:708	arg1	profiles					726:733	two-dimensional electrophoretic profiles	694:733	two-dimensional electrophoretic profiles	694:733	The mono- and two-dimensional electrophoretic profiles of Prosopis spp.
31000229	6	40	theme	C.	980:981	arg1	siliqua					983:989	C. siliqua	980:989	C. siliqua	980:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	9	41	theme	acids	1420:1424	arg1	acids					1420:1424	free amino acids	1409:1424	free amino acids	1409:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	41	theme	acids	1420:1424	arg1	peptides					1477:1484	low molecular weight peptides	1456:1484	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	41	theme	acids	1420:1424	arg1	amounts					1398:1404	high amounts	1393:1404	high amounts of free amino acids	1393:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	41	theme	acids	1420:1424	arg1	proportions					1441:1451	only minor proportions	1430:1451	only minor proportions of low molecular weight peptides	1430:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	42	theme	properties	181:190	arg1	virtue					124:129	virtue	124:129	virtue of exclusive nutrient composition and nutritional properties	124:190	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	0	43	dep	Ceratonia	65:73	arg1	L.					83:84	Ceratonia siliqua L.	65:84	Ceratonia siliqua L.	65:84	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	2	44	theme	high	373:376	arg1	value					390:394	a high nutritional value	371:394	a high nutritional value	371:394	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	44	theme	high	373:376	arg1	potential					358:366	potential	358:366	potential	358:366	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	9	45	theme	simulated	1345:1353	arg1	digestion					1372:1380	in vitro simulated gastrointestinal digestion	1336:1380	in vitro simulated gastrointestinal digestion	1336:1380	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	46	theme	nutritional	169:179	arg1	properties					181:190	nutritional properties	169:190	nutritional properties	169:190	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	9	47	theme	low	1456:1458	arg1	weight					1470:1475	low molecular weight	1456:1475	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	48	dep	carob	229:233	arg1	nigra					315:319	Prosopis nigra	306:319	Prosopis nigra	306:319	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	48	dep	carob	229:233	arg1	alba					297:300	Prosopis alba	288:300	Prosopis alba	288:300	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	0	49	theme	composition	32:42	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis	0:97	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	9	50	theme	gastrointestinal	1355:1370	arg1	digestion					1372:1380	in vitro simulated gastrointestinal digestion	1336:1380	in vitro simulated gastrointestinal digestion	1336:1380	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	51	theme	weight	1470:1475	arg1	peptides					1477:1484	low molecular weight peptides	1456:1484	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	52	contain	have	353:356	arg2	value					390:394	a high nutritional value	371:394	a high nutritional value	371:394	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	52	contain	have	353:356	arg2	potential					358:366	potential	358:366	potential	358:366	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	52	contain	have	353:356	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	53	theme	European	220:227	arg1	carob					229:233	European carob	220:233	European carob (Ceratonia siliqua L.)	220:256	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	53	theme	European	220:227	arg1	L.					254:255	L.	254:255	L.	254:255	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	9	54	theme	amino	1414:1418	arg1	acids					1420:1424	free amino acids	1409:1424	free amino acids	1409:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	55	theme	food	432:435	arg1	formulations					437:448	food formulations	432:448	food formulations	432:448	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	6	56	theme	structural	952:961	arg1	conformation					963:974	a dominant β-sheet structural conformation	933:974	a dominant β-sheet structural conformation for C. siliqua	933:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	2	57	theme	composition	153:163	arg1	virtue					124:129	virtue	124:129	virtue of exclusive nutrient composition and nutritional properties	124:190	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	9	58	theme	peptides	1477:1484	arg1	acids					1420:1424	free amino acids	1409:1424	free amino acids	1409:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	58	theme	peptides	1477:1484	arg1	peptides					1477:1484	low molecular weight peptides	1456:1484	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	58	theme	peptides	1477:1484	arg1	amounts					1398:1404	high amounts	1393:1404	high amounts of free amino acids	1393:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	58	theme	peptides	1477:1484	arg1	proportions					1441:1451	only minor proportions	1430:1451	only minor proportions of low molecular weight peptides	1430:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	1	59	theme	germ	109:112	arg1	flour					114:118	germ flour	109:118	germ flour	109:118	seed germ flour.
31000229	0	60	theme	digestibility	48:60	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis	0:97	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	6	61	theme	β-sheet	944:950	arg1	conformation					963:974	a dominant β-sheet structural conformation	933:974	a dominant β-sheet structural conformation for C. siliqua	933:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	9	62	theme	flour	1279:1283	arg1	samples					1285:1291	Both carob and algarrobo germ flour samples	1249:1291	Both carob and algarrobo germ flour samples	1249:1291	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	63	theme	carob	1254:1258	arg1	samples					1285:1291	Both carob and algarrobo germ flour samples	1249:1291	Both carob and algarrobo germ flour samples	1249:1291	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	3	64	theme	carob	561:565	arg1	content					550:556	the germ protein content	533:556	the germ protein content of carob	533:565	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	3	65	theme	compositional	476:488	arg1	properties					505:514	their compositional and functional properties	470:514	their compositional and functional properties	470:514	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	6	66	theme	Attenuated	841:850	arg1	reflectance-Fourier					858:876	Attenuated total reflectance-Fourier	841:876	Attenuated total reflectance-Fourier	841:876	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	6	67	theme	dominant	935:942	arg1	conformation					963:974	a dominant β-sheet structural conformation	933:974	a dominant β-sheet structural conformation for C. siliqua	933:989	Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob germ flour might more suited than Prosopis germ flour for baking and food technological applications.
31000229	2	68	theme	germ	198:201	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	3	69	theme	alba	586:589	arg1	counterparts					619:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	the P. alba, P. nigra and P. ruscifolia counterparts	579:630	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	2	70	theme	Prosopis	306:313	arg1	nigra					315:319	Prosopis nigra	306:319	Prosopis nigra	306:319	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	9	71	theme	high	1393:1396	arg1	acids					1420:1424	free amino acids	1409:1424	free amino acids	1409:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	71	theme	high	1393:1396	arg1	peptides					1477:1484	low molecular weight peptides	1456:1484	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	71	theme	high	1393:1396	arg1	amounts					1398:1404	high amounts	1393:1404	high amounts of free amino acids	1393:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	72	theme	seed	193:196	arg1	flours					203:208	seed germ flours	193:208	seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia)	193:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	8	73	theme	C.	1192:1193	arg1	siliqua					1195:1201	C. siliqua	1192:1201	C. siliqua	1192:1201	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	8	74	theme	amino	1226:1230	arg1	acid					1232:1235	essential amino acid	1216:1235	essential amino acid complement	1216:1246	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31000229	9	75	theme	germ	1274:1277	arg1	flour					1279:1283	algarrobo germ flour	1264:1283	algarrobo germ flour	1264:1283	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	9	76	theme	molecular	1460:1468	arg1	weight					1470:1475	low molecular weight	1456:1475	low molecular weight peptides	1456:1484	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	2	77	theme	Prosopis	332:339	arg1	vinal					325:329	vinal	325:329	vinal (Prosopis ruscifolia)	325:351	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	2	77	theme	Prosopis	332:339	arg1	ruscifolia					341:350	Prosopis ruscifolia	332:350	Prosopis ruscifolia	332:350	By virtue of exclusive nutrient composition and nutritional properties, seed germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations.
31000229	0	78	theme	Ceratonia	65:73	arg1	digestibility					48:60	digestibility	48:60	digestibility	48:60	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	0	78	theme	Ceratonia	65:73	arg1	Prosopis					90:97	Prosopis	90:97	Prosopis	90:97	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	0	78	theme	Ceratonia	65:73	arg1	composition					32:42	protein composition	24:42	protein composition	24:42	Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp.
31000229	3	79	theme	complementary	657:669	arg1	methods					671:677	proteomics and complementary methods	642:677	methods	671:677	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	5	80	theme	C.	795:796	arg1	siliqua					798:804	C. siliqua	795:804	C. siliqua	795:804	were very similar among one another, while C. siliqua exhibited significant differences.
31000229	3	81	theme	functional	494:503	arg1	properties					505:514	their compositional and functional properties	470:514	their compositional and functional properties	470:514	In order to define their compositional and functional properties, we investigated the germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods.
31000229	9	82	theme	free	1409:1412	arg1	acids					1420:1424	free amino acids	1409:1424	free amino acids	1409:1424	Both carob and algarrobo germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
31000229	8	83	theme	adequate	1160:1167	arg1	value					1181:1185	a more adequate nutritional value	1153:1185	a more adequate nutritional value than C. siliqua	1153:1201	contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement.
31349142	4	0	theme	acid	1024:1027	arg1	cluster					1042:1048	the legionaminic acid biosynthesis cluster	1007:1048	the legionaminic acid biosynthesis cluster	1007:1048	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	8	1	theme	present	1530:1536	arg1	consistent					1558:1567	consistent	1558:1567	consistent	1558:1567	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	1	theme	present	1530:1536	arg1	genes					1524:1528	the genes	1520:1528	the genes present at the K locus	1520:1551	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	0	2	theme	di-N-acyl	151:159	arg1	derivatives					161:171	different di-N-acyl derivatives	141:171	different di-N-acyl derivatives of legionaminic acid	141:192	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	1	3	theme	K54	233:235	arg1	polysaccharides					246:260	The K8 and K54 capsular polysaccharides	222:260	polysaccharides	246:260	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	2	4	theme	tetrasaccharide	566:580	arg1	repeats					582:588	related branched tetrasaccharide repeats	549:588	related branched tetrasaccharide repeats (K units) of the polysaccharides	549:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	2	4	theme	tetrasaccharide	566:580	arg1	units					593:597	K units	591:597	K units	591:597	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	4	5	from	O-7	930:932	arg1	difference					898:907	The difference	894:907	The difference in the acyl group at O-7 on the sidechain legionaminic acid	894:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	5	from	O-7	930:932	arg1	group					921:925	the acyl group	912:925	the acyl group at O-7 on the sidechain legionaminic acid	912:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	5	from	O-7	930:932	arg1	due					972:974	due	972:974	due	972:974	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	0	6	theme	acid	189:192	arg1	derivatives					161:171	different di-N-acyl derivatives	141:171	different di-N-acyl derivatives of legionaminic acid	141:192	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	8	7	theme	K8	1612:1613	arg1	structures					1594:1603	structures	1594:1603	structures	1594:1603	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	7	theme	K8	1612:1613	arg1	composition					1578:1588	composition	1578:1588	composition	1578:1588	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	1	8	theme	2-methyl-1-propanol	450:468	arg1	presence					438:445	the presence	434:445	the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy	434:518	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	8	9	theme	K54	1619:1621	arg1	polysaccharides					1632:1646	the K8 and K54 capsular polysaccharides	1608:1646	polysaccharides	1632:1646	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	4	10	theme	acyl	916:919	arg1	group					921:925	the acyl group	912:925	the acyl group at O-7 on the sidechain legionaminic acid	912:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	8	11	theme	polysaccharides	1632:1646	arg1	structures					1594:1603	structures	1594:1603	structures	1594:1603	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	11	theme	polysaccharides	1632:1646	arg1	composition					1578:1588	composition	1578:1588	composition	1578:1588	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	1	12	theme	partial	369:375	arg1	hydrolysis					382:391	partial acid hydrolysis	369:391	partial acid hydrolysis	369:391	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	2	13	theme	related	549:555	arg1	repeats					582:588	related branched tetrasaccharide repeats	549:588	related branched tetrasaccharide repeats (K units) of the polysaccharides	549:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	2	13	theme	related	549:555	arg1	units					593:597	K units	591:597	K units	591:597	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	8	14	dep	composition	1578:1588	arg1	the					1574:1576	the	1574:1576	the	1574:1576	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	3	15	theme	capsule	850:856	arg1	clusters					863:870	the KL8 and KL54 capsule gene clusters	833:870	the KL8 and KL54 capsule gene clusters	833:870	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	5	16	theme	first	1178:1182	arg1	sugar					1184:1188	the first sugar	1174:1188	the first sugar of both K units	1174:1204	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	5	16	theme	first	1178:1182	arg1	d-GlcpNAc					1226:1234	d-GlcpNAc	1226:1234	d-GlcpNAc	1226:1234	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	2	17	theme	5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic	661:725	arg1	acid					742:745	5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid	661:745	5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid	661:745	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	6	18	theme	Gtr20	1248:1252	arg1	glycosyltransferase					1254:1272	The shared Gtr20 glycosyltransferase	1237:1272	The shared Gtr20 glycosyltransferase	1237:1272	The shared Gtr20 glycosyltransferase, also encoded in KL63, forms the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage, and Gtr19 was predicted to form α-d-GalpNAc-(1 → 3)-l-FucpNAc.
31349142	1	19	theme	selective	397:405	arg1	solvolysis					407:416	selective solvolysis	397:416	selective solvolysis with CF3CO2H	397:429	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	6	20	theme	-d-GlcpNAc	1326:1335	arg1	linkage					1337:1343	the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage	1303:1343	the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage	1303:1343	The shared Gtr20 glycosyltransferase, also encoded in KL63, forms the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage, and Gtr19 was predicted to form α-d-GalpNAc-(1 → 3)-l-FucpNAc.
31349142	7	21	theme	Leg	1485:1487	arg1	derivative					1489:1498	the Leg derivative	1481:1498	the Leg derivative	1481:1498	Gtr18 from KL8 is 75% identical to Gtr108 from KL54 and both would link the Leg derivative to d-GalpNAc.
31349142	3	22	theme	clusters	863:870	arg1	sequences					820:828	The sequences	816:828	The sequences of the KL8 and KL54 capsule gene clusters	816:870	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	3	22	theme	clusters	863:870	arg1	related					885:891	related	885:891	related	885:891	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	2	23	from	~2.5:1	808:813	arg1	-3-hydroxybutanoyl					766:783	-3-hydroxybutanoyl	766:783	-3-hydroxybutanoyl	766:783	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	1	24	dep	1D	482:483	arg1	1H					492:493	1H	492:493	1H	492:493	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	24	dep	1D	482:483	arg1	spectroscopy					507:518	13C NMR spectroscopy	499:518	13C NMR spectroscopy	499:518	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	4	25	theme	sidechain	941:949	arg1	acid					964:967	the sidechain legionaminic acid	937:967	the sidechain legionaminic acid	937:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	8	26	from	locus	1547:1551	arg1	present					1530:1536	present	1530:1536	present	1530:1536	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	5	27	theme	unit	1080:1083	arg1	polymerases					1085:1095	the K unit polymerases	1074:1095	the K unit polymerases	1074:1095	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	1	28	with	hydrolysis	382:391	arg1	CF3CO2H					423:429	CF3CO2H	423:429	CF3CO2H	423:429	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	0	29	theme	K	0:0	arg1	units					2:6	K units	0:6	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52	0:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	4	30	theme	legionaminic	1011:1022	arg1	cluster					1042:1048	the legionaminic acid biosynthesis cluster	1007:1048	the legionaminic acid biosynthesis cluster	1007:1048	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	8	31	with	consistent	1558:1567	arg1	structures					1594:1603	structures	1594:1603	structures	1594:1603	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	31	with	consistent	1558:1567	arg1	composition					1578:1588	composition	1578:1588	composition	1578:1588	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	1	32	with	analysis	359:366	arg1	CF3CO2H					423:429	CF3CO2H	423:429	CF3CO2H	423:429	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	0	33	contain	have	105:108	arg1	units					2:6	K units	0:6	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52	0:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	33	contain	have	105:108	arg2	structure					119:127	the same structure	110:127	the same structure	110:127	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	34	theme	K8	15:16	arg1	units					2:6	K units	0:6	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52	0:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	35	theme	polysaccharides	35:49	arg1	units					2:6	K units	0:6	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52	0:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	2	36	from	-3-hydroxybutanoyl	766:783	arg1	~2.5:1					808:813	the ratio ~2.5:1	798:813	the ratio ~2.5:1	798:813	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	0	37	theme	K54	22:24	arg1	polysaccharides					35:49	the K8 and K54 capsular polysaccharides	11:49	polysaccharides	35:49	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	4	38	from	differences	979:989	arg1	genes					998:1002	two genes	994:1002	two genes in the legionaminic acid biosynthesis cluster	994:1048	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	8	39	theme	K	1545:1545	arg1	locus					1547:1551	the K locus	1541:1551	the K locus	1541:1551	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	5	40	theme	K	1198:1198	arg1	units					1200:1204	both K units	1193:1204	both K units	1193:1204	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	2	41	dep	-3-hydroxybutanoyl	766:783	arg1	R					764:764	R	764:764	R	764:764	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	4	42	theme	biosynthesis	1029:1040	arg1	cluster					1042:1048	the legionaminic acid biosynthesis cluster	1007:1048	the legionaminic acid biosynthesis cluster	1007:1048	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	6	43	theme	shared	1241:1246	arg1	glycosyltransferase					1254:1272	The shared Gtr20 glycosyltransferase	1237:1272	The shared Gtr20 glycosyltransferase	1237:1272	The shared Gtr20 glycosyltransferase, also encoded in KL63, forms the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage, and Gtr19 was predicted to form α-d-GalpNAc-(1 → 3)-l-FucpNAc.
31349142	1	44	theme	13C	499:501	arg1	spectroscopy					507:518	13C NMR spectroscopy	499:518	13C NMR spectroscopy	499:518	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	2	45	theme	repeats	582:588	arg1	structures					535:544	The following structures	521:544	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides	521:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	0	46	theme	legionaminic	176:187	arg1	acid					189:192	legionaminic acid	176:192	legionaminic acid	176:192	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	1	47	attach	isolated	267:274	arg2	polysaccharides					246:260	The K8 and K54 capsular polysaccharides	222:260	polysaccharides	246:260	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	47	attach	isolated	267:274	arg2	K8					226:227	The K8 and K54 capsular polysaccharides	222:260	K8	226:227	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	47	attach	isolated	267:274	arg1	baumannii					295:303	Acinetobacter baumannii	281:303	Acinetobacter baumannii BAL 097 and RCH52	281:321	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	48	theme	capsular	237:244	arg1	polysaccharides					246:260	The K8 and K54 capsular polysaccharides	222:260	polysaccharides	246:260	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	2	49	theme	branched	557:564	arg1	repeats					582:588	related branched tetrasaccharide repeats	549:588	related branched tetrasaccharide repeats (K units) of the polysaccharides	549:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	2	49	theme	branched	557:564	arg1	units					593:597	K units	591:597	K units	591:597	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	7	50	from	KL54	1456:1459	arg1	Gtr108					1444:1449	Gtr108	1444:1449	Gtr108 from KL54	1444:1459	Gtr18 from KL8 is 75% identical to Gtr108 from KL54 and both would link the Leg derivative to d-GalpNAc.
31349142	2	51	theme	K	591:591	arg1	repeats					582:588	related branched tetrasaccharide repeats	549:588	related branched tetrasaccharide repeats (K units) of the polysaccharides	549:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	2	51	theme	K	591:591	arg1	units					593:597	K units	591:597	K units	591:597	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	0	52	theme	same	114:117	arg1	structure					119:127	the same structure	110:127	the same structure	110:127	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	4	53	from	group	921:925	arg1	acid					964:967	the sidechain legionaminic acid	937:967	the sidechain legionaminic acid	937:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	7	54	attach	link	1476:1479	arg1	d-GalpNAc					1503:1511	d-GalpNAc	1503:1511	d-GalpNAc	1503:1511	Gtr18 from KL8 is 75% identical to Gtr108 from KL54 and both would link the Leg derivative to d-GalpNAc.
31349142	7	54	attach	link	1476:1479	arg2	both					1465:1468	both	1465:1468	both	1465:1468	Gtr18 from KL8 is 75% identical to Gtr108 from KL54 and both would link the Leg derivative to d-GalpNAc.
31349142	8	55	theme	capsular	1623:1630	arg1	polysaccharides					1632:1646	the K8 and K54 capsular polysaccharides	1608:1646	polysaccharides	1632:1646	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	1	56	theme	acid	377:380	arg1	hydrolysis					382:391	partial acid hydrolysis	369:391	partial acid hydrolysis	369:391	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	7	57	from	KL8	1420:1422	arg1	Gtr18					1409:1413	Gtr18	1409:1413	Gtr18 from KL8	1409:1422	Gtr18 from KL8 is 75% identical to Gtr108 from KL54 and both would link the Leg derivative to d-GalpNAc.
31349142	0	58	theme	different	141:149	arg1	derivatives					161:171	different di-N-acyl derivatives	141:171	different di-N-acyl derivatives of legionaminic acid	141:192	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	4	59	from	difference	898:907	arg1	acid					964:967	the sidechain legionaminic acid	937:967	the sidechain legionaminic acid	937:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	59	from	difference	898:907	arg1	O-7					930:932	O-7	930:932	O-7	930:932	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	59	from	difference	898:907	arg1	group					921:925	the acyl group	912:925	the acyl group at O-7 on the sidechain legionaminic acid	912:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	3	60	theme	gene	858:861	arg1	clusters					863:870	the KL8 and KL54 capsule gene clusters	833:870	the KL8 and KL54 capsule gene clusters	833:870	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	2	61	theme	following	525:533	arg1	structures					535:544	The following structures	521:544	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides	521:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	3	62	theme	KL54	845:848	arg1	clusters					863:870	the KL8 and KL54 capsule gene clusters	833:870	the KL8 and KL54 capsule gene clusters	833:870	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	4	63	theme	legionaminic	951:962	arg1	acid					964:967	the sidechain legionaminic acid	937:967	the sidechain legionaminic acid	937:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	3	64	theme	KL8	837:839	arg1	clusters					863:870	the KL8 and KL54 capsule gene clusters	833:870	the KL8 and KL54 capsule gene clusters	833:870	The sequences of the KL8 and KL54 capsule gene clusters were closely related.
31349142	0	65	theme	BAL	87:89	arg1	097					91:93	BAL 097	87:93	BAL 097	87:93	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	2	66	theme	polysaccharides	607:621	arg1	repeats					582:588	related branched tetrasaccharide repeats	549:588	related branched tetrasaccharide repeats (K units) of the polysaccharides	549:621	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	2	66	theme	polysaccharides	607:621	arg1	units					593:597	K units	591:597	K units	591:597	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	0	67	contain	contain	133:139	arg1	units					2:6	K units	0:6	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52	0:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	67	contain	contain	133:139	arg2	derivatives					161:171	different di-N-acyl derivatives	141:171	different di-N-acyl derivatives of legionaminic acid	141:192	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	1	68	theme	NMR	503:505	arg1	spectroscopy					507:518	13C NMR spectroscopy	499:518	13C NMR spectroscopy	499:518	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	69	with	solvolysis	407:416	arg1	CF3CO2H					423:429	CF3CO2H	423:429	CF3CO2H	423:429	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	70	dep	baumannii	295:303	arg1	BAL					305:307	BAL 097 and RCH52	305:321	BAL	305:307	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	70	dep	baumannii	295:303	arg1	RCH52					317:321	RCH52	317:321	RCH52	317:321	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	6	71	theme	α-d-GalpNAc-	1378:1389	arg1	-l-FucpNAc					1397:1406	α-d-GalpNAc-(1 → 3)-l-FucpNAc	1378:1406	α-d-GalpNAc-(1 → 3)-l-FucpNAc	1378:1406	The shared Gtr20 glycosyltransferase, also encoded in KL63, forms the α-l-FucpNAc-(1 → 3)-d-GlcpNAc linkage, and Gtr19 was predicted to form α-d-GalpNAc-(1 → 3)-l-FucpNAc.
31349142	1	72	theme	sugar	353:357	arg1	analysis					359:366	sugar analysis	353:366	sugar analysis	353:366	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	8	73	attach	present	1530:1536	arg2	consistent					1558:1567	consistent	1558:1567	consistent	1558:1567	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	73	attach	present	1530:1536	arg2	genes					1524:1528	the genes	1520:1528	the genes present at the K locus	1520:1551	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	8	73	attach	present	1530:1536	arg1	locus					1547:1551	the K locus	1541:1551	the K locus	1541:1551	Hence the genes present at the K locus were consistent with the composition and structures of the K8 and K54 capsular polysaccharides.
31349142	0	74	dep	Acinetobacter	63:75	arg1	RCH52					99:103	RCH52	99:103	RCH52	99:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	74	dep	Acinetobacter	63:75	arg1	097					91:93	BAL 097	87:93	BAL 097	87:93	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	0	74	dep	Acinetobacter	63:75	arg1	baumannii					77:85	Acinetobacter baumannii BAL 097 and RCH52	63:103	Acinetobacter baumannii BAL 097 and RCH52	63:103	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	5	75	theme	K	1078:1078	arg1	polymerases					1085:1095	the K unit polymerases	1074:1095	the K unit polymerases	1074:1095	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	5	76	theme	wzy	1055:1057	arg1	different					1106:1114	different	1106:1114	different	1106:1114	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	5	76	theme	wzy	1055:1057	arg1	genes					1059:1063	The wzy genes	1051:1063	The wzy genes encoding the K unit polymerases	1051:1095	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	5	77	theme	K	1156:1156	arg1	units					1158:1162	the K units	1152:1162	the K units	1152:1162	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	2	78	dep	5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic	661:725	arg1	legionaminic					728:739	legionaminic	728:739	legionaminic	728:739	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	4	79	from	genes	998:1002	arg1	cluster					1042:1048	the legionaminic acid biosynthesis cluster	1007:1048	the legionaminic acid biosynthesis cluster	1007:1048	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	80	from	acid	964:967	arg1	difference					898:907	The difference	894:907	The difference in the acyl group at O-7 on the sidechain legionaminic acid	894:967	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	4	80	from	acid	964:967	arg1	due					972:974	due	972:974	due	972:974	The difference in the acyl group at O-7 on the sidechain legionaminic acid is due to differences in two genes in the legionaminic acid biosynthesis cluster.
31349142	0	81	theme	capsular	26:33	arg1	polysaccharides					35:49	the K8 and K54 capsular polysaccharides	11:49	polysaccharides	35:49	K units of the K8 and K54 capsular polysaccharides produced by Acinetobacter baumannii BAL 097 and RCH52 have the same structure but contain different di-N-acyl derivatives of legionaminic acid and are linked differently.
31349142	5	82	theme	different	1125:1133	arg1	linkages					1135:1142	different linkages	1125:1142	different linkages between the K units	1125:1162	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	1	83	theme	1D	482:483	arg1	presence					438:445	the presence	434:445	the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy	434:518	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	1	84	dep	along	471:475	arg1	with					477:480	with	477:480	with	477:480	The K8 and K54 capsular polysaccharides were isolated from Acinetobacter baumannii BAL 097 and RCH52, respectively, and studied by sugar analysis, partial acid hydrolysis and selective solvolysis with CF3CO2H in the presence of 2-methyl-1-propanol, along with 1D and 2D 1H and 13C NMR spectroscopy.
31349142	2	85	theme	ratio	802:806	arg1	~2.5:1					808:813	the ratio ~2.5:1	798:813	the ratio ~2.5:1	798:813	The following structures of related branched tetrasaccharide repeats (K units) of the polysaccharides were established: where Leg indicates 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid and R indicates (R)-3-hydroxybutanoyl or acetyl in the ratio ~2.5:1.
31349142	5	86	theme	units	1200:1204	arg1	sugar					1184:1188	the first sugar	1174:1188	the first sugar of both K units	1174:1204	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31349142	5	86	theme	units	1200:1204	arg1	d-GlcpNAc					1226:1234	d-GlcpNAc	1226:1234	d-GlcpNAc	1226:1234	The wzy genes encoding the K unit polymerases are also different and make different linkages between the K units, allowing the first sugar of both K units to be identified as d-GlcpNAc.
31630309	0	0	theme	date	78:81	arg1	seeds					88:92	date palm seeds	78:92	date palm seeds	78:92	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
31630309	3	1	theme	treated	593:599	arg1	seeds					601:605	the treated seeds	589:605	the treated seeds	589:605	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	1	2	from	effects	151:157	arg1	composition					233:243	fiber composition	227:243	fiber composition	227:243	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	1	2	from	effects	151:157	arg1	degradability					255:267	rumen degradability	249:267	rumen degradability	249:267	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	10	3	theme	%	1557:1557	arg1	NaOH					1559:1562	4% NaOH	1556:1562	4% NaOH	1556:1562	In conclusion, our results showed that treating DPS with 4% NaOH increased the seeds' ruminal degradability, and subjecting DPS to sonication further improved their degradability in the rumen.
31630309	6	4	theme	unsonicated	1055:1065	arg1	seeds					1067:1071	unsonicated seeds	1055:1071	unsonicated seeds	1055:1071	The degradability of seeds' NDF and OM were greater (P < 0.05) for the sonicated than unsonicated seeds.
31630309	2	5	theme	first	302:306	arg1	trial					308:312	the first trial	298:312	the first trial	298:312	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	6	6	dep	greater	1013:1019	arg1	P < 0.05					1022:1029	P < 0.05	1022:1029	P < 0.05	1022:1029	The degradability of seeds' NDF and OM were greater (P < 0.05) for the sonicated than unsonicated seeds.
31630309	1	7	theme	fiber	227:231	arg1	composition					233:243	fiber composition	227:243	fiber composition	227:243	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	0	8	theme	seeds	88:92	arg1	degradability					61:73	the rumen degradability	51:73	the rumen degradability of date palm seeds	51:92	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
31630309	4	9	theme	untreated	672:680	arg1	seeds					682:686	untreated seeds	672:686	untreated seeds	672:686	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	5	10	theme	second	855:860	arg1	trial					862:866	the second trial	851:866	the second trial	851:866	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	7	11	dep	seen	1108:1111	arg1	whereas					1138:1144	whereas	1138:1144	whereas	1138:1144	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	0	12	theme	palm	83:86	arg1	seeds					88:92	date palm seeds	78:92	date palm seeds	78:92	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
31630309	10	13	theme	ruminal	1585:1591	arg1	degradability					1593:1605	the seeds' ruminal degradability	1574:1605	the seeds' ruminal degradability	1574:1605	In conclusion, our results showed that treating DPS with 4% NaOH increased the seeds' ruminal degradability, and subjecting DPS to sonication further improved their degradability in the rumen.
31630309	10	14	with	DPS	1547:1549	arg1	NaOH					1559:1562	4% NaOH	1556:1562	4% NaOH	1556:1562	In conclusion, our results showed that treating DPS with 4% NaOH increased the seeds' ruminal degradability, and subjecting DPS to sonication further improved their degradability in the rumen.
31630309	7	15	theme	NDF	1086:1088	arg1	degradability					1090:1102	The highest NDF degradability	1074:1102	The highest NDF degradability	1074:1102	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	8	16	theme	third	1222:1226	arg1	trial					1228:1232	the third trial	1218:1232	the third trial	1218:1232	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	9	17	theme	%	1491:1491	arg1	NaOH					1493:1496	the 4% NaOH	1486:1496	the 4% NaOH	1486:1496	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	8	18	from	sonication	1268:1277	arg1	solutions					1297:1305	different NaOH solutions	1282:1305	different NaOH solutions (1%, 2%, 4% NaOH)	1282:1323	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	8	19	theme	NaOH	1292:1295	arg1	solutions					1297:1305	different NaOH solutions	1282:1305	different NaOH solutions (1%, 2%, 4% NaOH)	1282:1323	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	4	20	theme	lignin	711:716	arg1	content					718:724	lignin content	711:724	lignin content	711:724	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	3	21	theme	untreated	460:468	arg1	seeds					470:474	untreated seeds	460:474	untreated seeds	460:474	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	2	22	theme	incubation	330:339	arg1	effects					319:325	the effects	315:325	the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability	315:430	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	4	23	dep	lower	731:735	arg1	P < 0.05					738:745	P < 0.05	738:745	P < 0.05	738:745	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	8	24	theme	different	1282:1290	arg1	solutions					1297:1305	different NaOH solutions	1282:1305	different NaOH solutions (1%, 2%, 4% NaOH)	1282:1323	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	9	25	theme	untreated	1353:1361	arg1	seeds					1363:1367	untreated seeds	1353:1367	untreated seeds	1353:1367	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	1	26	theme	rumen	249:253	arg1	degradability					255:267	rumen degradability	249:267	rumen degradability	249:267	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	10	27	theme	treating	1538:1545	arg1	DPS					1547:1549	treating DPS	1538:1549	treating DPS with 4% NaOH	1538:1562	In conclusion, our results showed that treating DPS with 4% NaOH increased the seeds' ruminal degradability, and subjecting DPS to sonication further improved their degradability in the rumen.
31630309	7	28	theme	sonication	1187:1196	arg1	P > 0.05					1204:1211	P > 0.05	1204:1211	P > 0.05	1204:1211	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	7	28	theme	sonication	1187:1196	arg1	time					1198:1201	sonication time	1187:1201	sonication time (P > 0.05)	1187:1212	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	1	29	theme	chemical	162:169	arg1	treatment					171:179	chemical treatment	162:179	chemical treatment	162:179	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	9	30	theme	4	1490:1490	arg1	%					1491:1491	%	1491:1491	%	1491:1491	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	9	31	theme	seeds	1397:1401	arg1	degradability					1380:1392	the rumen degradability	1370:1392	the rumen degradability of seeds' NDF and OM	1370:1413	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	9	31	theme	seeds	1397:1401	arg1	highest					1462:1468	highest	1462:1468	highest	1462:1468	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	7	32	theme	OM	1150:1151	arg1	degradability					1153:1165	the OM degradability	1146:1165	the OM degradability	1146:1165	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	4	33	theme	incubated	831:839	arg1	seeds					841:845	the incubated seeds	827:845	the incubated seeds	827:845	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	1	34	theme	treatment	171:179	arg1	effects					151:157	the effects	147:157	the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS)	147:292	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	2	35	theme	sodium	361:366	arg1	NaOH					379:382	NaOH	379:382	NaOH	379:382	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	35	theme	sodium	361:366	arg1	hydroxide					368:376	4% sodium hydroxide	358:376	4% sodium hydroxide (NaOH)	358:383	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	8	36	dep	%	1317:1317	arg1	NaOH					1319:1322	NaOH	1319:1322	4% NaOH	1316:1322	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	9	37	dep	seeds	1397:1401	arg1	NDF					1404:1406	NDF	1404:1406	NDF	1404:1406	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	9	37	dep	seeds	1397:1401	arg1	seeds					1397:1401	seeds	1397:1401	seeds' NDF and OM	1397:1413	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	9	37	dep	seeds	1397:1401	arg1	OM					1412:1413	OM	1412:1413	OM	1412:1413	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	3	38	theme	ruminal	481:487	arg1	degradability					489:501	the ruminal degradability	477:501	the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM)	477:562	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	38	theme	ruminal	481:487	arg1	highest					616:622	highest	616:622	highest	616:622	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	2	39	theme	4	358:358	arg1	%					359:359	%	359:359	%	359:359	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	40	theme	fiber	392:396	arg1	content					398:404	DPS fiber content	388:404	DPS fiber content	388:404	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	1	41	theme	date	272:275	arg1	seeds					282:286	date palm seeds	272:286	date palm seeds (DPS)	272:292	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	1	41	theme	date	272:275	arg1	DPS					289:291	DPS	289:291	DPS	289:291	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	4	42	dep	higher	805:810	arg1	P < 0.05					813:820	P < 0.05	813:820	P < 0.05	813:820	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	5	43	theme	different	902:910	arg1	times					923:927	different sonication times	902:927	different sonication times (5, 10, 20, and 30 min)	902:951	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	3	44	theme	detergent	518:526	arg1	NDF					535:537	NDF	535:537	NDF	535:537	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	44	theme	detergent	518:526	arg1	fiber					528:532	DPS neutral detergent fiber	506:532	DPS neutral detergent fiber (NDF)	506:538	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	2	45	from	sonication	344:353	arg1	NaOH					379:382	NaOH	379:382	NaOH	379:382	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	45	from	sonication	344:353	arg1	hydroxide					368:376	4% sodium hydroxide	358:376	4% sodium hydroxide (NaOH)	358:383	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	1	46	theme	sonication	185:194	arg1	processing					209:218	sonication (ultrasound) processing	185:218	sonication (ultrasound) processing	185:218	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	0	47	theme	sonication	23:32	arg1	Effect					0:5	Effect	0:5	Effect of alkaline and sonication	0:32	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
31630309	1	48	theme	palm	277:280	arg1	seeds					282:286	date palm seeds	272:286	date palm seeds (DPS)	272:292	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	1	48	theme	palm	277:280	arg1	DPS					289:291	DPS	289:291	DPS	289:291	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	2	49	theme	DPS	388:390	arg1	content					398:404	DPS fiber content	388:404	DPS fiber content	388:404	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	3	50	theme	organic	544:550	arg1	OM					560:561	OM	560:561	OM	560:561	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	50	theme	organic	544:550	arg1	matter					552:557	organic matter	544:557	organic matter (OM)	544:562	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	51	theme	fiber	528:532	arg1	degradability					489:501	the ruminal degradability	477:501	the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM)	477:562	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	51	theme	fiber	528:532	arg1	highest					616:622	highest	616:622	highest	616:622	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	2	52	theme	sonication	344:353	arg1	effects					319:325	the effects	315:325	the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability	315:430	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	1	53	theme	seeds	282:286	arg1	composition					233:243	fiber composition	227:243	fiber composition	227:243	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	1	53	theme	seeds	282:286	arg1	degradability					255:267	rumen degradability	249:267	rumen degradability	249:267	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	4	54	theme	sonicated	756:764	arg1	seeds					766:770	the sonicated seeds	752:770	the sonicated seeds	752:770	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	2	55	theme	ruminal	410:416	arg1	degradability					418:430	ruminal degradability	410:430	ruminal degradability	410:430	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	3	56	theme	DPS	506:508	arg1	NDF					535:537	NDF	535:537	NDF	535:537	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	56	theme	DPS	506:508	arg1	fiber					528:532	DPS neutral detergent fiber	506:532	DPS neutral detergent fiber (NDF)	506:538	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	2	57	from	effects	319:325	arg1	NaOH					379:382	NaOH	379:382	NaOH	379:382	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	57	from	effects	319:325	arg1	content					398:404	DPS fiber content	388:404	DPS fiber content	388:404	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	57	from	effects	319:325	arg1	degradability					418:430	ruminal degradability	410:430	ruminal degradability	410:430	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	57	from	effects	319:325	arg1	hydroxide					368:376	4% sodium hydroxide	358:376	4% sodium hydroxide (NaOH)	358:383	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	1	58	theme	main	99:102	arg1	objective					104:112	The main objective	95:112	The main objective of this research	95:129	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	4	59	theme	cellulose	783:791	arg1	higher					805:810	higher	805:810	higher	805:810	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	4	59	theme	cellulose	783:791	arg1	content					793:799	the cellulose content	779:799	the cellulose content	779:799	Relative to untreated seeds, the hemicellulose and lignin content were lower (P < 0.05) for the sonicated seeds, while the cellulose content was higher (P < 0.05) for the incubated seeds.
31630309	9	60	theme	rumen	1374:1378	arg1	degradability					1380:1392	the rumen degradability	1370:1392	the rumen degradability of seeds' NDF and OM	1370:1413	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	9	60	theme	rumen	1374:1378	arg1	highest					1462:1468	highest	1462:1468	highest	1462:1468	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	1	61	dep	composition	233:243	arg1	the					223:225	the	223:225	the	223:225	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	3	62	theme	neutral	510:516	arg1	NDF					535:537	NDF	535:537	NDF	535:537	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	62	theme	neutral	510:516	arg1	fiber					528:532	DPS neutral detergent fiber	506:532	DPS neutral detergent fiber (NDF)	506:538	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	10	63	theme	4	1556:1556	arg1	%					1557:1557	%	1557:1557	%	1557:1557	In conclusion, our results showed that treating DPS with 4% NaOH increased the seeds' ruminal degradability, and subjecting DPS to sonication further improved their degradability in the rumen.
31630309	5	64	theme	sonication	912:921	arg1	times					923:927	different sonication times	902:927	different sonication times (5, 10, 20, and 30 min)	902:951	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	3	65	theme	sonicated	643:651	arg1	seeds					653:657	the sonicated seeds	639:657	the sonicated seeds	639:657	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	9	66	dep	highest	1462:1468	arg1	P < 0.05					1471:1478	P < 0.05	1471:1478	P < 0.05	1471:1478	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	2	67	from	incubation	330:339	arg1	NaOH					379:382	NaOH	379:382	NaOH	379:382	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	67	from	incubation	330:339	arg1	hydroxide					368:376	4% sodium hydroxide	358:376	4% sodium hydroxide (NaOH)	358:383	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	3	68	theme	matter	552:557	arg1	degradability					489:501	the ruminal degradability	477:501	the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM)	477:562	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	3	68	theme	matter	552:557	arg1	highest					616:622	highest	616:622	highest	616:622	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	1	69	theme	ultrasound	197:206	arg1	processing					209:218	sonication (ultrasound) processing	185:218	sonication (ultrasound) processing	185:218	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	3	70	dep	increased	564:572	arg1	P < 0.05					575:582	P < 0.05	575:582	P < 0.05	575:582	Relative to untreated seeds, the ruminal degradability of DPS neutral detergent fiber (NDF) and organic matter (OM) increased (P < 0.05) for the treated seeds and were highest (P < 0.05) for the sonicated seeds.
31630309	6	71	dep	seeds	990:994	arg1	NDF					997:999	NDF	997:999	NDF	997:999	The degradability of seeds' NDF and OM were greater (P < 0.05) for the sonicated than unsonicated seeds.
31630309	6	71	dep	seeds	990:994	arg1	seeds					990:994	seeds' NDF and OM	990:1006	seeds' NDF and OM	990:1006	The degradability of seeds' NDF and OM were greater (P < 0.05) for the sonicated than unsonicated seeds.
31630309	6	71	dep	seeds	990:994	arg1	OM					1005:1006	OM	1005:1006	OM	1005:1006	The degradability of seeds' NDF and OM were greater (P < 0.05) for the sonicated than unsonicated seeds.
31630309	5	72	dep	times	923:927	arg1	20					937:938	20	937:938	20	937:938	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	5	72	dep	times	923:927	arg1	30 min					945:950	30 min	945:950	30 min	945:950	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	5	72	dep	times	923:927	arg1	10					933:934	10	933:934	10	933:934	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	5	72	dep	times	923:927	arg1	5					930:930	5	930:930	5	930:930	In the second trial, the effects of subjecting DPS to different sonication times (5, 10, 20, and 30 min) were evaluated.
31630309	9	73	theme	NaOH	1434:1437	arg1	concentrations					1439:1452	all NaOH concentrations	1430:1452	all NaOH concentrations	1430:1452	Relative to untreated seeds, the rumen degradability of seeds' NDF and OM increased with all NaOH concentrations but was highest (P < 0.05) with the 4% NaOH.
31630309	8	74	dep	solutions	1297:1305	arg1	%					1309:1309	1%	1308:1309	1%	1308:1309	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	8	74	dep	solutions	1297:1305	arg1	%					1313:1313	2%	1312:1313	2%	1312:1313	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	8	74	dep	solutions	1297:1305	arg1	%					1317:1317	4%	1316:1317	4% NaOH	1316:1322	In the third trial, the effects of subjecting DPS to sonication in different NaOH solutions (1%, 2%, 4% NaOH) were evaluated.
31630309	7	75	theme	30 min	1119:1124	arg1	sonication					1126:1135	30 min sonication	1119:1135	30 min sonication	1119:1135	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	2	76	theme	%	359:359	arg1	NaOH					379:382	NaOH	379:382	NaOH	379:382	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	2	76	theme	%	359:359	arg1	hydroxide					368:376	4% sodium hydroxide	358:376	4% sodium hydroxide (NaOH)	358:383	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	1	77	theme	research	122:129	arg1	objective					104:112	The main objective	95:112	The main objective of this research	95:129	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	7	78	theme	highest	1078:1084	arg1	degradability					1090:1102	The highest NDF degradability	1074:1102	The highest NDF degradability	1074:1102	The highest NDF degradability was seen after 30 min sonication, whereas the OM degradability was not affected by sonication time (P > 0.05).
31630309	1	79	theme	processing	209:218	arg1	effects					151:157	the effects	147:157	the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS)	147:292	The main objective of this research was to evaluate the effects of chemical treatment and sonication (ultrasound) processing on the fiber composition and rumen degradability of date palm seeds (DPS).
31630309	0	80	theme	rumen	55:59	arg1	degradability					61:73	the rumen degradability	51:73	the rumen degradability of date palm seeds	51:92	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
31630309	2	81	from	hydroxide	368:376	arg1	effects					319:325	the effects	315:325	the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability	315:430	In the first trial, the effects of incubation or sonication in 4% sodium hydroxide (NaOH) on DPS fiber content and ruminal degradability were evaluated.
31630309	0	82	theme	alkaline	10:17	arg1	Effect					0:5	Effect	0:5	Effect of alkaline and sonication	0:32	Effect of alkaline and sonication pretreatments on the rumen degradability of date palm seeds.
29458100	1	0	theme	boletus	178:184	arg1	mushrooms					186:194	thirteen boletus mushrooms	169:194	thirteen boletus mushrooms representing five different species collected in Southwest China	169:259	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	7	1	theme	natural	1185:1191	arg1	luridus					1058:1064	Suillellus luridus	1047:1064	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China	1047:1110	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	7	1	theme	natural	1185:1191	arg1	antioxidant					1193:1203	a natural antioxidant	1183:1203	a natural antioxidant	1183:1203	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	6	2	theme	significant	908:918	arg1	correlation					920:930	a significant correlation	906:930	a significant correlation with their monosaccharide composition	906:968	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	1	3	theme	mushrooms	186:194	arg1	stipes					159:164	stipes	159:164	stipes	159:164	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	1	3	theme	mushrooms	186:194	arg1	caps					150:153	caps	150:153	caps	150:153	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	4	4	from	stipes	672:677	arg1	ranges					620:625	the molecular weight ranges	599:625	the molecular weight ranges of the polysaccharides from the caps and the stipes	599:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	4	from	stipes	672:677	arg1	polysaccharides					634:648	the polysaccharides	630:648	the polysaccharides from the caps and the stipes	630:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	0	5	from	Characterization	0:15	arg1	mushrooms					85:93	thirteen boletus mushrooms	68:93	thirteen boletus mushrooms	68:93	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	5	6	theme	FT-IR	680:684	arg1	analysis					695:702	FT-IR spectral analysis	680:702	FT-IR spectral analysis of the polysaccharides	680:725	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	7	7	theme	superior	1127:1134	arg1	activity					1148:1155	superior antioxidant activity	1127:1155	superior antioxidant activity	1127:1155	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	5	8	theme	spectral	686:693	arg1	analysis					695:702	FT-IR spectral analysis	680:702	FT-IR spectral analysis of the polysaccharides	680:725	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	5	9	theme	pyranose	824:831	arg1	ring					833:836	a pyranose ring	822:836	a pyranose ring	822:836	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	7	10	contain	had	1112:1114	arg1	luridus					1058:1064	Suillellus luridus	1047:1064	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China	1047:1110	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	7	10	contain	had	1112:1114	arg1	antioxidant					1193:1203	a natural antioxidant	1183:1203	a natural antioxidant	1183:1203	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	7	10	contain	had	1112:1114	arg2	activity					1148:1155	superior antioxidant activity	1127:1155	superior antioxidant activity	1127:1155	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	4	11	from	caps	659:662	arg1	ranges					620:625	the molecular weight ranges	599:625	the molecular weight ranges of the polysaccharides from the caps and the stipes	599:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	11	from	caps	659:662	arg1	polysaccharides					634:648	the polysaccharides	630:648	the polysaccharides from the caps and the stipes	630:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	12	theme	significant	558:568	arg1	differences					570:580	significant differences	558:580	significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes	558:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	7	13	theme	Suillellus	1047:1056	arg1	luridus					1058:1064	Suillellus luridus	1047:1064	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China	1047:1110	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	7	13	theme	Suillellus	1047:1056	arg1	antioxidant					1193:1203	a natural antioxidant	1183:1203	a natural antioxidant	1183:1203	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
29458100	1	14	theme	Water-soluble	96:108	arg1	polysaccharides					110:124	Water-soluble polysaccharides	96:124	Water-soluble polysaccharides	96:124	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	6	15	theme	anomeric	1023:1030	arg1	configuration					1032:1044	anomeric configuration	1023:1044	anomeric configuration	1023:1044	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	5	16	contain	contained	812:820	arg1	most					741:744	most	741:744	most	741:744	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	5	16	contain	contained	812:820	arg2	ring					833:836	a pyranose ring	822:836	a pyranose ring	822:836	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	5	16	contain	contained	812:820	arg1	polysaccharides					749:763	polysaccharides	749:763	polysaccharides from boletus mushrooms (except Boletus edulis)	749:810	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	5	17	theme	polysaccharides	711:725	arg1	analysis					695:702	FT-IR spectral analysis	680:702	FT-IR spectral analysis of the polysaccharides	680:725	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	6	18	theme	polysaccharides	873:887	arg1	related					985:991	related	985:991	related	985:991	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	6	18	theme	polysaccharides	873:887	arg1	activities					855:864	The antioxidant activities	839:864	The antioxidant activities of the polysaccharides in stipes	839:897	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	1	19	theme	different	214:222	arg1	species					224:230	five different species	209:230	five different species collected in Southwest China	209:259	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	4	20	theme	polysaccharides	634:648	arg1	ranges					620:625	the molecular weight ranges	599:625	the molecular weight ranges of the polysaccharides from the caps and the stipes	599:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	6	21	theme	monosaccharide	943:956	arg1	composition					958:968	their monosaccharide composition	937:968	their monosaccharide composition	937:968	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	2	22	theme	relationships	368:380	arg1	evaluation					335:344	an evaluation	332:344	an evaluation of structure-function relationships	332:380	Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships.
29458100	0	23	theme	antioxidant	21:31	arg1	activities					33:42	antioxidant activities	21:42	antioxidant activities	21:42	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	5	24	theme	Boletus	796:802	arg1	edulis					804:809	Boletus edulis	796:809	Boletus edulis	796:809	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	4	25	theme	broad	523:527	arg1	range					546:550	a broad molecular weight range	521:550	a broad molecular weight range	521:550	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	2	26	theme	structure-function	349:366	arg1	relationships					368:380	structure-function relationships	349:380	structure-function relationships	349:380	Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships.
29458100	6	27	with	correlation	920:930	arg1	composition					958:968	their monosaccharide composition	937:968	their monosaccharide composition	937:968	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	0	28	theme	polysaccharides	47:61	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	0	28	theme	polysaccharides	47:61	arg1	activities					33:42	antioxidant activities	21:42	antioxidant activities	21:42	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	1	29	theme	Southwest	245:253	arg1	China					255:259	Southwest China	245:259	Southwest China	245:259	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	4	30	from	ranges	620:625	arg1	caps					659:662	the caps	655:662	the caps	655:662	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	30	from	ranges	620:625	arg1	stipes					672:677	the stipes	668:677	the stipes	668:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	31	theme	weight	613:618	arg1	ranges					620:625	the molecular weight ranges	599:625	the molecular weight ranges of the polysaccharides from the caps and the stipes	599:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	32	theme	weight	539:544	arg1	range					546:550	a broad molecular weight range	521:550	a broad molecular weight range	521:550	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	6	33	from	polysaccharides	873:887	arg1	stipes					892:897	stipes	892:897	stipes	892:897	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	4	34	theme	molecular	603:611	arg1	ranges					620:625	the molecular weight ranges	599:625	the molecular weight ranges of the polysaccharides from the caps and the stipes	599:677	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	4	35	theme	molecular	529:537	arg1	range					546:550	a broad molecular weight range	521:550	a broad molecular weight range	521:550	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	6	36	theme	antioxidant	843:853	arg1	related					985:991	related	985:991	related	985:991	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	6	36	theme	antioxidant	843:853	arg1	activities					855:864	The antioxidant activities	839:864	The antioxidant activities of the polysaccharides in stipes	839:897	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	6	37	theme	molecular	1002:1010	arg1	weight					1012:1017	their molecular weight	996:1017	their molecular weight	996:1017	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	5	38	theme	boletus	770:776	arg1	mushrooms					778:786	boletus mushrooms	770:786	boletus mushrooms (except Boletus edulis)	770:810	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	4	39	theme	Most	498:501	arg1	samples					503:509	Most samples	498:509	Most samples	498:509	Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes.
29458100	0	40	from	mushrooms	85:93	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from thirteen boletus mushrooms	47:93	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	0	40	from	mushrooms	85:93	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	0	40	from	mushrooms	85:93	arg1	activities					33:42	antioxidant activities	21:42	antioxidant activities	21:42	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	3	41	dep	monosaccharides	431:445	arg1	arabinose					447:455	arabinose	447:455	arabinose	447:455	The polysaccharides were composed mainly of the monosaccharides arabinose, xylose, mannose, glucose and galactose.
29458100	2	42	theme	activities	313:322	arg1	Investigations					262:275	Investigations	262:275	Investigations of their structures and antioxidant activities	262:322	Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships.
29458100	6	43	from	activities	855:864	arg1	stipes					892:897	stipes	892:897	stipes	892:897	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	5	44	from	mushrooms	778:786	arg1	polysaccharides					749:763	polysaccharides	749:763	polysaccharides from boletus mushrooms (except Boletus edulis)	749:810	FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring.
29458100	1	45	dep	caps	150:153	arg1	the					146:148	the	146:148	the	146:148	Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China.
29458100	2	46	theme	antioxidant	301:311	arg1	activities					313:322	antioxidant activities	301:322	antioxidant activities	301:322	Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships.
29458100	6	47	from	stipes	892:897	arg1	related					985:991	related	985:991	related	985:991	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	6	47	from	stipes	892:897	arg1	activities					855:864	The antioxidant activities	839:864	The antioxidant activities of the polysaccharides in stipes	839:897	The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration.
29458100	0	48	theme	boletus	77:83	arg1	mushrooms					85:93	thirteen boletus mushrooms	68:93	thirteen boletus mushrooms	68:93	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	0	49	from	activities	33:42	arg1	mushrooms					85:93	thirteen boletus mushrooms	68:93	thirteen boletus mushrooms	68:93	Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.
29458100	2	50	theme	structures	286:295	arg1	Investigations					262:275	Investigations	262:275	Investigations of their structures and antioxidant activities	262:322	Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships.
29458100	7	51	theme	antioxidant	1136:1146	arg1	activity					1148:1155	superior antioxidant activity	1127:1155	superior antioxidant activity	1127:1155	Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant.
31121211	0	0	theme	intracerebral	91:103	arg1	hemorrhage					105:114	intracerebral hemorrhage	91:114	intracerebral hemorrhage	91:114	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	8	1	theme	reduction	1098:1106	arg1	terms					1077:1081	terms	1077:1081	terms of brain edema reduction and neural apoptosis inhibition	1077:1138	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	2	2	theme	drug	361:364	arg1	bioavailability					366:380	the drug bioavailability	357:380	the drug bioavailability	357:380	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	8	3	theme	edema	1092:1096	arg1	reduction					1098:1106	brain edema reduction	1086:1106	brain edema reduction	1086:1106	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	6	4	theme	HAMC	776:779	arg1	gel					781:783	the HAMC gel	772:783	the HAMC gel	772:783	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	2	5	from	transport	319:327	arg1	gel					343:345	intranasal gel	332:345	intranasal gel	332:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	8	6	theme	neural	1112:1117	arg1	inhibition					1129:1138	neural apoptosis inhibition	1112:1138	neural apoptosis inhibition	1112:1138	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	8	7	theme	CS	1005:1006	arg1	NPs + HAMC					1008:1017	CS NPs + HAMC	1005:1017	CS NPs + HAMC	1005:1017	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	9	8	theme	intranasal	1326:1335	arg1	system					1351:1356	this intranasal drug delivery system	1321:1356	this intranasal drug delivery system	1321:1356	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	4	9	theme	chitosan	597:604	arg1	nanoparticles					606:618	chitosan nanoparticles	597:618	chitosan nanoparticles (CS NPs)	597:627	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	4	9	theme	chitosan	597:604	arg1	NPs					624:626	CS NPs	621:626	CS NPs	621:626	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	2	10	theme	absorption	289:298	arg1	site					300:303	the absorption site	285:303	the absorption site	285:303	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	8	11	theme	inhibition	1129:1138	arg1	terms					1077:1081	terms	1077:1081	terms of brain edema reduction and neural apoptosis inhibition	1077:1138	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	9	12	theme	ICH	1435:1437	arg1	treatment					1422:1430	the treatment	1418:1430	the treatment of ICH	1418:1437	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	6	13	theme	NPs	765:767	arg1	migration					749:757	The oriented migration	736:757	The oriented migration of CS NPs in the HAMC gel	736:783	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	3	14	theme	intranasal	432:441	arg1	gel					443:445	a novel intranasal gel	424:445	a novel intranasal gel with oriented drug migration	424:474	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	8	15	theme	apoptosis	1119:1127	arg1	inhibition					1129:1138	neural apoptosis inhibition	1112:1138	neural apoptosis inhibition	1112:1138	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	8	16	theme	NPs + HAMC	1008:1017	arg1	composite					1023:1031	the CS NPs + HAMC (+) composite	1001:1031	the CS NPs + HAMC (+) composite	1001:1031	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	6	17	theme	CS	762:763	arg1	NPs					765:767	CS NPs	762:767	CS NPs	762:767	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	7	18	theme	CS	946:947	arg1	composites					960:969	CS NPs + HAMC composites	946:969	CS NPs + HAMC composites	946:969	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	2	19	theme	ICH	397:399	arg1	outcome					386:392	outcome	386:392	outcome	386:392	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	2	19	theme	ICH	397:399	arg1	bioavailability					366:380	the drug bioavailability	357:380	the drug bioavailability	357:380	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	0	20	theme	enhanced	3:10	arg1	delivery					37:44	An enhanced charge-driven intranasal delivery	0:44	An enhanced charge-driven intranasal delivery of nicardipine	0:59	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	8	21	theme	brain	1086:1090	arg1	reduction					1098:1106	brain edema reduction	1086:1106	brain edema reduction	1086:1106	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	9	22	theme	therapeutic	1393:1403	arg1	strategy					1405:1412	a new therapeutic strategy	1387:1412	a new therapeutic strategy for the treatment of ICH	1387:1437	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	8	23	theme	stronger	1042:1049	arg1	effect					1067:1072	a stronger neuroprotective effect	1040:1072	a stronger neuroprotective effect	1040:1072	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	7	24	theme	ICH	878:880	arg1	model					886:890	a blood-induced ICH rat model	862:890	a blood-induced ICH rat model	862:890	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	2	25	theme	drug	314:317	arg1	transport					319:327	slow drug transport	309:327	slow drug transport in intranasal gel	309:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	3	26	theme	novel	426:430	arg1	gel					443:445	a novel intranasal gel	424:445	a novel intranasal gel with oriented drug migration	424:474	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	0	27	theme	intranasal	26:35	arg1	delivery					37:44	An enhanced charge-driven intranasal delivery	0:44	An enhanced charge-driven intranasal delivery of nicardipine	0:59	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	8	28	theme	Intranasal	972:981	arg1	administration					983:996	Intranasal administration	972:996	Intranasal administration of the CS NPs + HAMC (+) composite	972:1031	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	6	29	theme	oriented	740:747	arg1	migration					749:757	The oriented migration	736:757	The oriented migration of CS NPs in the HAMC gel	736:783	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	2	30	theme	slow	309:312	arg1	transport					319:327	slow drug transport	309:327	slow drug transport in intranasal gel	309:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	9	31	contain	has	1358:1360	arg1	system					1351:1356	this intranasal drug delivery system	1321:1356	this intranasal drug delivery system	1321:1356	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	9	31	contain	has	1358:1360	arg2	potential					1366:1374	the potential to provide a new therapeutic strategy for the treatment of ICH	1362:1437	the potential to provide a new therapeutic strategy for the treatment of ICH	1362:1437	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	1	32	theme	intracerebral	216:228	arg1	hemorrhage					230:239	an intracerebral hemorrhage	213:239	an intracerebral hemorrhage (ICH)	213:245	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	1	32	theme	intracerebral	216:228	arg1	ICH					242:244	ICH	242:244	ICH	242:244	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	0	33	theme	charge-driven	12:24	arg1	delivery					37:44	An enhanced charge-driven intranasal delivery	0:44	An enhanced charge-driven intranasal delivery of nicardipine	0:59	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	9	34	theme	charge-driven	1293:1305	arg1	strategy					1307:1314	the charge-driven strategy	1289:1314	the charge-driven strategy	1289:1314	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	8	35	theme	composite	1023:1031	arg1	administration					983:996	Intranasal administration	972:996	Intranasal administration of the CS NPs + HAMC (+) composite	972:1031	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	1	36	theme	drug	128:131	arg1	delivery					133:140	Intranasal drug delivery	117:140	Intranasal drug delivery	117:140	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	2	37	from	time	277:280	arg1	gel					343:345	intranasal gel	332:345	intranasal gel	332:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	1	38	theme	hemorrhage	230:239	arg1	intervention					197:208	the intervention	193:208	the intervention of an intracerebral hemorrhage (ICH)	193:245	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	9	39	from	nose	1253:1256	arg1	transport					1238:1246	the oriented and rapid drug transport	1210:1246	the oriented and rapid drug transport from nose to brain	1210:1265	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	6	40	from	migration	749:757	arg1	gel					781:783	the HAMC gel	772:783	the HAMC gel	772:783	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	5	41	theme	gel	689:691	arg1	side					677:680	one side	673:680	one side of the gel	673:691	Subsequently, one side of the gel was coated with a positively charged film.
31121211	6	42	theme	drug	809:812	arg1	bioavailability					814:828	the drug bioavailability	805:828	the drug bioavailability	805:828	The oriented migration of CS NPs in the HAMC gel was determined, and the drug bioavailability was also enhanced.
31121211	4	43	theme	HAMC	649:652	arg1	gel					654:656	an HAMC gel	646:656	an HAMC gel	646:656	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	8	44	theme	NPs + HAMC	1159:1168	arg1	composite					1170:1178	the CS NPs + HAMC composite	1152:1178	the CS NPs + HAMC composite	1152:1178	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	8	45	theme	CS	1156:1157	arg1	composite					1170:1178	the CS NPs + HAMC composite	1152:1178	the CS NPs + HAMC composite	1152:1178	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	3	46	theme	oriented	452:459	arg1	migration					466:474	oriented drug migration	452:474	oriented drug migration	452:474	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	9	47	theme	new	1389:1391	arg1	strategy					1405:1412	a new therapeutic strategy	1387:1412	a new therapeutic strategy for the treatment of ICH	1387:1437	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	3	48	theme	charge-driven	488:500	arg1	strategy					502:509	a charge-driven strategy	486:509	a charge-driven strategy to attenuate brain injury after ICH	486:545	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	0	49	theme	nicardipine	49:59	arg1	delivery					37:44	An enhanced charge-driven intranasal delivery	0:44	An enhanced charge-driven intranasal delivery of nicardipine	0:59	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	4	50	theme	Nicardipine	548:558	arg1	NCD					575:577	NCD	575:577	NCD	575:577	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	4	50	theme	Nicardipine	548:558	arg1	hydrochloride					560:572	Nicardipine hydrochloride	548:572	Nicardipine hydrochloride (NCD)	548:578	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	8	51	theme	neuroprotective	1051:1065	arg1	effect					1067:1072	a stronger neuroprotective effect	1040:1072	a stronger neuroprotective effect	1040:1072	Intranasal administration of the CS NPs + HAMC (+) composite showed a stronger neuroprotective effect in terms of brain edema reduction and neural apoptosis inhibition compared to the CS NPs + HAMC composite.
31121211	9	52	theme	oriented	1214:1221	arg1	transport					1238:1246	the oriented and rapid drug transport	1210:1246	the oriented and rapid drug transport from nose to brain	1210:1265	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	7	53	theme	NPs + HAMC	949:958	arg1	composites					960:969	CS NPs + HAMC composites	946:969	CS NPs + HAMC composites	946:969	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	7	54	theme	rat	882:884	arg1	model					886:890	a blood-induced ICH rat model	862:890	a blood-induced ICH rat model	862:890	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	7	55	theme	composites	960:969	arg1	effect					936:941	the therapeutic effect	920:941	the therapeutic effect of CS NPs + HAMC composites	920:969	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	2	56	theme	intranasal	332:341	arg1	gel					343:345	intranasal gel	332:345	intranasal gel	332:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	1	57	theme	Intranasal	117:126	arg1	delivery					133:140	Intranasal drug delivery	117:140	Intranasal drug delivery	117:140	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	9	58	theme	drug	1337:1340	arg1	system					1351:1356	this intranasal drug delivery system	1321:1356	this intranasal drug delivery system	1321:1356	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	3	59	with	gel	443:445	arg1	migration					466:474	oriented drug migration	452:474	oriented drug migration	452:474	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	9	60	theme	rapid	1227:1231	arg1	transport					1238:1246	the oriented and rapid drug transport	1210:1246	the oriented and rapid drug transport from nose to brain	1210:1265	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	2	61	theme	retention	267:275	arg1	time					277:280	the short retention time	257:280	the short retention time at the absorption site	257:303	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	1	62	theme	alternative	154:164	arg1	approach					180:187	an alternative and effective approach	151:187	an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH)	151:245	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	2	63	from	site	300:303	arg1	transport					319:327	slow drug transport	309:327	slow drug transport in intranasal gel	309:345	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	2	63	from	site	300:303	arg1	time					277:280	the short retention time	257:280	the short retention time at the absorption site	257:303	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	9	64	theme	delivery	1342:1349	arg1	system					1351:1356	this intranasal drug delivery system	1321:1356	this intranasal drug delivery system	1321:1356	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	5	65	theme	charged	722:728	arg1	film					730:733	a positively charged film	709:733	a positively charged film	709:733	Subsequently, one side of the gel was coated with a positively charged film.
31121211	9	66	theme	drug	1233:1236	arg1	transport					1238:1246	the oriented and rapid drug transport	1210:1246	the oriented and rapid drug transport from nose to brain	1210:1265	These results suggested that the oriented and rapid drug transport from nose to brain can be achieved using the charge-driven strategy, and this intranasal drug delivery system has the potential to provide a new therapeutic strategy for the treatment of ICH.
31121211	2	67	theme	short	261:265	arg1	time					277:280	the short retention time	257:280	the short retention time at the absorption site	257:303	However, the short retention time at the absorption site and slow drug transport in intranasal gel influence the drug bioavailability and outcome of ICH.
31121211	3	68	theme	drug	461:464	arg1	migration					466:474	oriented drug migration	452:474	oriented drug migration	452:474	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	3	69	theme	brain	524:528	arg1	injury					530:535	brain injury	524:535	brain injury	524:535	Herein, we fabricated a novel intranasal gel with oriented drug migration utilizing a charge-driven strategy to attenuate brain injury after ICH.
31121211	7	70	theme	blood-induced	864:876	arg1	model					886:890	a blood-induced ICH rat model	862:890	a blood-induced ICH rat model	862:890	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	0	71	theme	brain	72:76	arg1	injury					78:83	brain injury	72:83	brain injury	72:83	An enhanced charge-driven intranasal delivery of nicardipine attenuates brain injury after intracerebral hemorrhage.
31121211	7	72	theme	therapeutic	924:934	arg1	effect					936:941	the therapeutic effect	920:941	the therapeutic effect of CS NPs + HAMC composites	920:969	Furthermore, a blood-induced ICH rat model was established to evaluate the therapeutic effect of CS NPs + HAMC composites.
31121211	1	73	theme	effective	170:178	arg1	approach					180:187	an alternative and effective approach	151:187	an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH)	151:245	Intranasal drug delivery provided an alternative and effective approach for the intervention of an intracerebral hemorrhage (ICH).
31121211	4	74	theme	CS	621:622	arg1	nanoparticles					606:618	chitosan nanoparticles	597:618	chitosan nanoparticles (CS NPs)	597:627	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
31121211	4	74	theme	CS	621:622	arg1	NPs					624:626	CS NPs	621:626	CS NPs	621:626	Nicardipine hydrochloride (NCD) was entrapped in chitosan nanoparticles (CS NPs) and dispersed in an HAMC gel.
30516980	2	0	from	fruit	189:193	arg1	Polysaccharides					161:175	Polysaccharides	161:175	Polysaccharides from feijoa fruit	161:193	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	7	1	dep	structure	1192:1200	arg1	the					1188:1190	the	1188:1190	the	1188:1190	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	2	from	activities	1216:1225	arg1	gut					1258:1260	the human gut	1248:1260	the human gut	1248:1260	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	3	from	structure	1192:1200	arg1	gut					1258:1260	the human gut	1248:1260	the human gut	1248:1260	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	6	4	from	differences	936:946	arg1	preference					957:966	their preference	951:966	their preference for the two polysaccharides in this preparation	951:1014	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	7	5	from	gut	1258:1260	arg1	structure					1192:1200	structure	1192:1200	structure	1192:1200	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	5	from	gut	1258:1260	arg1	activities					1216:1225	metabolic activities	1206:1225	metabolic activities	1206:1225	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	3	6	theme	polysaccharide	358:371	arg1	extracts					373:380	The two major polysaccharide extracts	344:380	The two major polysaccharide extracts	344:380	The two major polysaccharide extracts consisted of mainly pectic polysaccharides and hemicellulosic polysaccharides [xyloglucan (77%) and arabinoxylan (16%)].
30516980	0	7	from	Characterization	0:15	arg1	Fruits					48:53	Feijoa Fruits	41:53	Feijoa Fruits	41:53	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	6	8	theme	Constituent	821:831	arg1	sugar					833:837	Constituent sugar and glycosyl linkage analysis	821:867	sugar	833:837	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	4	9	theme	polysaccharide	596:609	arg1	preparations					611:622	these polysaccharide preparations	590:622	these polysaccharide preparations	590:622	A collection of commensal Bacteroides species was screened for growth in culture using these polysaccharide preparations and placed into five categories based on their preference for each substrate.
30516980	6	10	theme	hemicellulose	906:918	arg1	fraction					920:927	the hemicellulose fraction	902:927	the hemicellulose fraction	902:927	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	5	11	theme	pectic	747:752	arg1	polysaccharides					754:768	the pectic polysaccharides	743:768	the pectic polysaccharides	743:768	Most of the species tested could utilize the pectic polysaccharides, but growth on the hemicellulose was more limited.
30516980	3	12	theme	major	352:356	arg1	extracts					373:380	The two major polysaccharide extracts	344:380	The two major polysaccharide extracts	344:380	The two major polysaccharide extracts consisted of mainly pectic polysaccharides and hemicellulosic polysaccharides [xyloglucan (77%) and arabinoxylan (16%)].
30516980	0	13	from	Fruits	48:53	arg1	Polysaccharides					20:34	Polysaccharides	20:34	Polysaccharides from Feijoa Fruits	20:53	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	0	13	from	Fruits	48:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)	0:78	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	0	13	from	Fruits	48:53	arg1	Berg					73:76	Berg	73:76	Berg	73:76	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	7	14	theme	microbiota	1234:1243	arg1	structure					1192:1200	structure	1192:1200	structure	1192:1200	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	14	theme	microbiota	1234:1243	arg1	activities					1216:1225	metabolic activities	1206:1225	metabolic activities	1206:1225	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	4	15	from	growth	566:571	arg1	culture					576:582	culture	576:582	culture using these polysaccharide preparations	576:622	A collection of commensal Bacteroides species was screened for growth in culture using these polysaccharide preparations and placed into five categories based on their preference for each substrate.
30516980	7	16	theme	metabolic	1206:1214	arg1	activities					1216:1225	metabolic activities	1206:1225	metabolic activities	1206:1225	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	17	theme	human	1252:1256	arg1	gut					1258:1260	the human gut	1248:1260	the human gut	1248:1260	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	18	theme	pectic	1112:1117	arg1	polysaccharides					1119:1133	pectic polysaccharides	1112:1133	pectic polysaccharides	1112:1133	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	3	19	theme	pectic	402:407	arg1	polysaccharides					409:423	mainly pectic polysaccharides	395:423	mainly pectic polysaccharides	395:423	The two major polysaccharide extracts consisted of mainly pectic polysaccharides and hemicellulosic polysaccharides [xyloglucan (77%) and arabinoxylan (16%)].
30516980	7	20	theme	polysaccharides	1119:1133	arg1	arabinoxylan					1152:1163	arabinoxylan	1152:1163	arabinoxylan	1152:1163	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	20	theme	polysaccharides	1119:1133	arg1	xyloglucan					1136:1145	xyloglucan	1136:1145	xyloglucan	1136:1145	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	20	theme	polysaccharides	1119:1133	arg1	hydrolysis					1098:1107	differential hydrolysis	1085:1107	differential hydrolysis of pectic polysaccharides	1085:1133	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	2	21	theme	cell	314:317	arg1	walls					319:323	the primary cell walls	302:323	the primary cell walls of eudicotyledons	302:341	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	6	22	from	polysaccharides	980:994	arg1	preparation					1004:1014	this preparation	999:1014	this preparation	999:1014	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	2	23	theme	primary	306:312	arg1	walls					319:323	the primary cell walls	302:323	the primary cell walls of eudicotyledons	302:341	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	0	24	theme	Polysaccharides	20:34	arg1	Characterization					0:15	Characterization	0:15	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)	0:78	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	0	24	theme	Polysaccharides	20:34	arg1	Berg					73:76	Berg	73:76	Berg	73:76	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	3	25	theme	hemicellulosic	429:442	arg1	polysaccharides					444:458	hemicellulosic polysaccharides	429:458	hemicellulosic polysaccharides	429:458	The two major polysaccharide extracts consisted of mainly pectic polysaccharides and hemicellulosic polysaccharides [xyloglucan (77%) and arabinoxylan (16%)].
30516980	2	26	theme	feijoa	182:187	arg1	fruit					189:193	feijoa fruit	182:193	feijoa fruit	182:193	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	0	27	theme	Feijoa	41:46	arg1	Fruits					48:53	Feijoa Fruits	41:53	Feijoa Fruits	41:53	Characterization of Polysaccharides from Feijoa Fruits ( Acca sellowiana Berg.)
30516980	2	28	located	found	293:297	arg2	those					277:281	those	277:281	those	277:281	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	2	28	located	found	293:297	arg1	walls					319:323	the primary cell walls	302:323	the primary cell walls of eudicotyledons	302:341	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	7	29	theme	differential	1085:1096	arg1	hydrolysis					1098:1107	differential hydrolysis	1085:1107	differential hydrolysis of pectic polysaccharides	1085:1133	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	7	30	from	microbiota	1234:1243	arg1	gut					1258:1260	the human gut	1248:1260	the human gut	1248:1260	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	4	31	theme	Bacteroides	529:539	arg1	species					541:547	commensal Bacteroides species	519:547	commensal Bacteroides species	519:547	A collection of commensal Bacteroides species was screened for growth in culture using these polysaccharide preparations and placed into five categories based on their preference for each substrate.
30516980	7	32	theme	genus	1062:1066	arg1	Bacteroides					1068:1078	the genus Bacteroides	1058:1078	the genus Bacteroides	1058:1078	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	1	33	theme	Growth	105:110	arg1	Substrates					112:121	Growth Substrates	105:121	Growth Substrates	105:121	and Their Utilization as Growth Substrates by Gut Commensal Bacteroides Species.
30516980	7	34	theme	Bacteroides	1068:1078	arg1	members					1047:1053	the members	1043:1053	the members of the genus Bacteroides	1043:1078	Our data demonstrate that the members of the genus Bacteroides show differential hydrolysis of pectic polysaccharides, xyloglucan, and arabinoxylan, which might influence the structure and metabolic activities of the microbiota in the human gut.
30516980	5	35	from	growth	775:780	arg1	hemicellulose					789:801	the hemicellulose	785:801	the hemicellulose	785:801	Most of the species tested could utilize the pectic polysaccharides, but growth on the hemicellulose was more limited.
30516980	4	36	theme	species	541:547	arg1	collection					505:514	A collection	503:514	A collection of commensal Bacteroides species	503:547	A collection of commensal Bacteroides species was screened for growth in culture using these polysaccharide preparations and placed into five categories based on their preference for each substrate.
30516980	6	37	theme	linkage	852:858	arg1	analysis					860:867	Constituent sugar and glycosyl linkage analysis	821:867	analysis	860:867	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	2	38	theme	eudicotyledons	328:341	arg1	walls					319:323	the primary cell walls	302:323	the primary cell walls of eudicotyledons	302:341	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	6	39	theme	glycosyl	843:850	arg1	analysis					860:867	Constituent sugar and glycosyl linkage analysis	821:867	analysis	860:867	Constituent sugar and glycosyl linkage analysis showed that species that grew on the hemicellulose fraction showed differences in their preference for the two polysaccharides in this preparation.
30516980	1	40	theme	Gut	126:128	arg1	Species					152:158	Gut Commensal Bacteroides Species	126:158	Gut Commensal Bacteroides Species	126:158	and Their Utilization as Growth Substrates by Gut Commensal Bacteroides Species.
30516980	2	41	theme	polysaccharides	249:263	arg1	composition					228:238	the composition	224:238	the composition of these polysaccharides	224:263	Polysaccharides from feijoa fruit were extracted and analyzed; the composition of these polysaccharides conforms to those typically found in the primary cell walls of eudicotyledons.
30516980	1	42	theme	Commensal	130:138	arg1	Species					152:158	Gut Commensal Bacteroides Species	126:158	Gut Commensal Bacteroides Species	126:158	and Their Utilization as Growth Substrates by Gut Commensal Bacteroides Species.
30516980	1	43	theme	Bacteroides	140:150	arg1	Species					152:158	Gut Commensal Bacteroides Species	126:158	Gut Commensal Bacteroides Species	126:158	and Their Utilization as Growth Substrates by Gut Commensal Bacteroides Species.
30516980	4	44	theme	commensal	519:527	arg1	species					541:547	commensal Bacteroides species	519:547	commensal Bacteroides species	519:547	A collection of commensal Bacteroides species was screened for growth in culture using these polysaccharide preparations and placed into five categories based on their preference for each substrate.
31527444	8	0	theme	HO-1	1579:1582	arg1	expression					1592:1601	HO-1 protein expression	1579:1601	HO-1 protein expression	1579:1601	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	5	1	theme	aging	698:702	arg1	model					710:714	d-galactose-induced aging mouse model	678:714	d-galactose-induced aging mouse model	678:714	The mechanism was investigated exploring d-galactose-induced aging mouse model.
31527444	8	2	theme	expression	1592:1601	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	ratio					1476:1480	raised Bcl-2/Bax ratio	1459:1480	raised Bcl-2/Bax ratio	1459:1480	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	PI3K					1324:1327	PI3K	1324:1327	PI3K	1324:1327	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	Akt					1533:1535	enhanced Akt	1524:1535	enhanced Akt	1524:1535	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	phosphorylation					1538:1552	phosphorylation	1538:1552	phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression	1538:1601	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	expression					1641:1650	Bax protein expression	1629:1650	Bax protein expression	1629:1650	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	O3a					1401:1403	forkhead box O3a	1388:1403	forkhead box O3a (FOXO3a)	1388:1412	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	Akt					1331:1333	Akt	1331:1333	Akt	1331:1333	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	3-kinase					1314:1321	upregulated phosphatidylinositol 3-kinase	1281:1321	upregulated phosphatidylinositol 3-kinase (PI3K)	1281:1328	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	caspase-3					1614:1622	decreased caspase-3	1604:1622	decreased caspase-3	1604:1622	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	2	theme	expression	1592:1601	arg1	FOXO3a					1406:1411	FOXO3a	1406:1411	FOXO3a	1406:1411	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	4	3	theme	experiments	409:419	arg1	series					399:404	A series	397:404	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy	389:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	8	4	theme	decreased	1604:1612	arg1	caspase-3					1614:1622	decreased caspase-3	1604:1622	decreased caspase-3	1604:1622	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	5	theme	Bax	1629:1631	arg1	expression					1641:1650	Bax protein expression	1629:1650	Bax protein expression	1629:1650	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	4	6	theme	monosaccharide	450:463	arg1	composition					465:475	monosaccharide composition	450:475	monosaccharide composition	450:475	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	8	7	theme	AMC	1194:1196	arg1	polysaccharides					1198:1212	AMC polysaccharides	1194:1212	AMC polysaccharides	1194:1212	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	7	8	theme	Glc	1022:1024	arg1	acid					1016:1019	glucuronic acid	1005:1019	glucuronic acid (Glc A)	1005:1027	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	7	8	theme	Glc	1022:1024	arg1	A					1026:1026	Glc A	1022:1026	Glc A	1022:1026	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	1	9	theme	Aging	137:141	arg1	Mice					143:146	d-Galactose-Induced Aging Mice	117:146	d-Galactose-Induced Aging Mice	117:146	Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway.
31527444	8	10	theme	box	1397:1399	arg1	O3a					1401:1403	forkhead box O3a	1388:1403	forkhead box O3a (FOXO3a)	1388:1412	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	10	theme	box	1397:1399	arg1	FOXO3a					1406:1411	FOXO3a	1406:1411	FOXO3a	1406:1411	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	11	theme	d-galactose-induced	1234:1252	arg1	changes					1254:1260	d-galactose-induced changes	1234:1260	d-galactose-induced changes in mice	1234:1268	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	4	12	dep	METHODS	389:395	arg1	series					399:404	A series	397:404	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy	389:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	0	13	theme	Athyrium	77:84	arg1	multidentatum					86:98	Athyrium multidentatum	77:98	Athyrium multidentatum	77:98	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	3	14	theme	protective	308:317	arg1	mechanism					319:327	the protective mechanism	304:327	the protective mechanism against d-galactose-induced oxidative stress in aging mice	304:386	Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.
31527444	9	15	theme	AMC	1664:1666	arg1	polysaccharides					1668:1682	CONCLUSION AMC polysaccharides	1653:1682	CONCLUSION AMC polysaccharides	1653:1682	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	8	16	theme	upregulated	1281:1291	arg1	PI3K					1324:1327	PI3K	1324:1327	PI3K	1324:1327	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	16	theme	upregulated	1281:1291	arg1	3-kinase					1314:1321	upregulated phosphatidylinositol 3-kinase	1281:1321	upregulated phosphatidylinositol 3-kinase (PI3K)	1281:1328	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	7	17	theme	0.077:0.088:0.09:1:0.375:0.354:0.04	1116:1150	arg1	ratio					1107:1111	a molar ratio	1099:1111	a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw)	1099:1191	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	3	18	theme	oxidative	357:365	arg1	stress					367:372	d-galactose-induced oxidative stress	337:372	d-galactose-induced oxidative stress in aging mice	337:386	Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.
31527444	6	19	theme	transcription	748:760	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	19	theme	transcription	748:760	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	2	20	from	multidentatum	250:262	arg1	polysaccharides					220:234	the polysaccharides	216:234	the polysaccharides from Athyrium multidentatum	216:262	The purpose of this study was to characterize the polysaccharides from Athyrium multidentatum (Doll.)
31527444	9	21	theme	oxidative	1715:1723	arg1	stress					1725:1730	d-galactose-induced oxidative stress	1695:1730	d-galactose-induced oxidative stress	1695:1730	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	0	22	from	Activity	44:51	arg1	multidentatum					86:98	Athyrium multidentatum	77:98	Athyrium multidentatum	77:98	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	6	23	theme	real-time	730:738	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	23	theme	real-time	730:738	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	0	24	theme	Structural	0:9	arg1	Doll					101:104	Doll	101:104	Doll	101:104	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	24	theme	Structural	0:9	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization	0:26	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	8	25	theme	raised	1459:1464	arg1	ratio					1476:1480	raised Bcl-2/Bax ratio	1459:1480	raised Bcl-2/Bax ratio	1459:1480	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	4	26	theme	1H	531:532	arg1	resonance					551:559	1H nuclear magnetic resonance	531:559	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	4	26	theme	1H	531:532	arg1	NMR					565:567	1H NMR	562:567	1H NMR	562:567	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	8	27	theme	factor-erythroid	1344:1359	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	27	theme	factor-erythroid	1344:1359	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	6	28	theme	western	802:808	arg1	blotting					810:817	western blotting	802:817	western blotting	802:817	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	0	29	theme	Polysaccharides	56:70	arg1	Doll					101:104	Doll	101:104	Doll	101:104	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	29	theme	Polysaccharides	56:70	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization	0:26	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	29	theme	Polysaccharides	56:70	arg1	Activity					44:51	Antioxidant Activity	32:51	Antioxidant Activity	32:51	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	9	30	theme	PI3K/AKT	1769:1776	arg1	pathway					1778:1784	the PI3K/AKT pathway	1765:1784	the PI3K/AKT pathway	1765:1784	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	8	31	theme	downregulated	1483:1495	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	ratio					1476:1480	raised Bcl-2/Bax ratio	1459:1480	raised Bcl-2/Bax ratio	1459:1480	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	PI3K					1324:1327	PI3K	1324:1327	PI3K	1324:1327	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	Akt					1533:1535	enhanced Akt	1524:1535	enhanced Akt	1524:1535	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	phosphorylation					1538:1552	phosphorylation	1538:1552	phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression	1538:1601	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	expression					1641:1650	Bax protein expression	1629:1650	Bax protein expression	1629:1650	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	O3a					1401:1403	forkhead box O3a	1388:1403	forkhead box O3a (FOXO3a)	1388:1412	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	Akt					1331:1333	Akt	1331:1333	Akt	1331:1333	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	3-kinase					1314:1321	upregulated phosphatidylinositol 3-kinase	1281:1321	upregulated phosphatidylinositol 3-kinase (PI3K)	1281:1328	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	caspase-3					1614:1622	decreased caspase-3	1604:1622	decreased caspase-3	1604:1622	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	32	theme	Akt	1557:1559	arg1	FOXO3a					1406:1411	FOXO3a	1406:1411	FOXO3a	1406:1411	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	33	theme	mRNA	1507:1510	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	2	34	dep	characterize	203:214	arg1	Doll					265:268	Doll	265:268	Doll	265:268	The purpose of this study was to characterize the polysaccharides from Athyrium multidentatum (Doll.)
31527444	6	35	theme	chain	773:777	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	35	theme	chain	773:777	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	7	36	theme	AMC	930:932	arg1	polysaccharides					934:948	AMC polysaccharides	930:948	AMC polysaccharides	930:948	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	4	37	dep	Fourier	478:484	arg1	transform					486:494	transform	486:494	transform infrared (FT-IR) spectroscopy	486:524	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	5	38	theme	d-galactose-induced	678:696	arg1	model					710:714	d-galactose-induced aging mouse model	678:714	d-galactose-induced aging mouse model	678:714	The mechanism was investigated exploring d-galactose-induced aging mouse model.
31527444	6	39	theme	gene	855:858	arg1	expression					872:881	the gene and protein expression	851:881	expression	872:881	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	7	40	dep	composed	962:969	arg1	Fuc					1091:1093	Fuc	1091:1093	Fuc	1091:1093	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	8	41	theme	protein	1584:1590	arg1	expression					1592:1601	HO-1 protein expression	1579:1601	HO-1 protein expression	1579:1601	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	9	42	theme	anti-aging	1828:1837	arg1	activity					1839:1846	their anti-aging activity	1822:1846	their anti-aging activity	1822:1846	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	4	43	theme	resonance	551:559	arg1	spectroscopy					570:581	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	7	44	dep	showed	918:923	arg1	FINDINGS					897:904	KEY FINDINGS	893:904	KEY FINDINGS	893:904	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	7	45	theme	KEY	893:895	arg1	FINDINGS					897:904	KEY FINDINGS	893:904	KEY FINDINGS	893:904	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	8	46	theme	hemeoxygenase-1	1419:1433	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	6	47	from	expression	872:881	arg1	liver					886:890	liver	886:890	liver	886:890	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	3	48	from	stress	367:372	arg1	mice					383:386	aging mice	377:386	aging mice	377:386	Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.
31527444	8	49	theme	forkhead	1388:1395	arg1	O3a					1401:1403	forkhead box O3a	1388:1403	forkhead box O3a (FOXO3a)	1388:1412	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	49	theme	forkhead	1388:1395	arg1	FOXO3a					1406:1411	FOXO3a	1406:1411	FOXO3a	1406:1411	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	7	50	with	ratio	1107:1111	arg1	weight					1169:1174	a molecular weight	1157:1174	a molecular weight of 33203 Da (Mw)	1157:1191	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	4	51	theme	magnetic	542:549	arg1	resonance					551:559	1H nuclear magnetic resonance	531:559	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	4	51	theme	magnetic	542:549	arg1	NMR					565:567	1H NMR	562:567	1H NMR	562:567	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	5	52	theme	mouse	704:708	arg1	model					710:714	d-galactose-induced aging mouse model	678:714	d-galactose-induced aging mouse model	678:714	The mechanism was investigated exploring d-galactose-induced aging mouse model.
31527444	8	53	theme	protein	1633:1639	arg1	expression					1641:1650	Bax protein expression	1629:1650	Bax protein expression	1629:1650	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	1	54	theme	d-Galactose-Induced	117:135	arg1	Mice					143:146	d-Galactose-Induced Aging Mice	117:146	d-Galactose-Induced Aging Mice	117:146	Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway.
31527444	4	55	theme	molecular	432:440	arg1	weight					442:447	molecular weight	432:447	molecular weight	432:447	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	7	56	theme	molecular	1159:1167	arg1	weight					1169:1174	a molecular weight	1157:1174	a molecular weight of 33203 Da (Mw)	1157:1191	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	7	57	theme	glucuronic	1005:1014	arg1	acid					1016:1019	glucuronic acid	1005:1019	glucuronic acid (Glc A)	1005:1027	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	7	57	theme	glucuronic	1005:1014	arg1	A					1026:1026	Glc A	1022:1026	Glc A	1022:1026	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	1	58	theme	PI3K/AKT	152:159	arg1	Pathway					161:167	PI3K/AKT Pathway	152:167	PI3K/AKT Pathway	152:167	Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway.
31527444	3	59	theme	d-galactose-induced	337:355	arg1	stress					367:372	d-galactose-induced oxidative stress	337:372	d-galactose-induced oxidative stress in aging mice	337:386	Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.
31527444	8	60	theme	HO-1	1436:1439	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	9	61	theme	CONCLUSION	1653:1662	arg1	polysaccharides					1668:1682	CONCLUSION AMC polysaccharides	1653:1682	CONCLUSION AMC polysaccharides	1653:1682	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	0	62	from	Characterization	11:26	arg1	multidentatum					86:98	Athyrium multidentatum	77:98	Athyrium multidentatum	77:98	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	3	63	theme	aging	377:381	arg1	mice					383:386	aging mice	377:386	aging mice	377:386	Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.
31527444	4	64	theme	infrared	496:503	arg1	spectroscopy					513:524	infrared (FT-IR) spectroscopy	496:524	infrared (FT-IR) spectroscopy	496:524	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	6	65	theme	polymerase	762:771	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	65	theme	polymerase	762:771	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	7	66	theme	molar	1101:1105	arg1	ratio					1107:1111	a molar ratio	1099:1111	a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw)	1099:1191	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	9	67	theme	d-galactose-induced	1695:1713	arg1	stress					1725:1730	d-galactose-induced oxidative stress	1695:1730	d-galactose-induced oxidative stress	1695:1730	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	4	68	theme	FT-IR	506:510	arg1	spectroscopy					513:524	infrared (FT-IR) spectroscopy	496:524	infrared (FT-IR) spectroscopy	496:524	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	8	69	theme	phosphatidylinositol	1293:1312	arg1	PI3K					1324:1327	PI3K	1324:1327	PI3K	1324:1327	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	69	theme	phosphatidylinositol	1293:1312	arg1	3-kinase					1314:1321	upregulated phosphatidylinositol 3-kinase	1281:1321	upregulated phosphatidylinositol 3-kinase (PI3K)	1281:1328	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	6	70	theme	reverse	740:746	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	70	theme	reverse	740:746	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	4	71	theme	1H	562:563	arg1	resonance					551:559	1H nuclear magnetic resonance	531:559	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	4	71	theme	1H	562:563	arg1	NMR					565:567	1H NMR	562:567	1H NMR	562:567	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	6	72	theme	Quantitative	717:728	arg1	RT-qPCR					789:795	RT-qPCR	789:795	RT-qPCR	789:795	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	6	72	theme	Quantitative	717:728	arg1	reaction					779:786	Quantitative real-time reverse transcription polymerase chain reaction	717:786	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR)	717:796	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	0	73	theme	Antioxidant	32:42	arg1	Activity					44:51	Antioxidant Activity	32:51	Antioxidant Activity	32:51	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	8	74	from	changes	1254:1260	arg1	mice					1265:1268	mice	1265:1268	mice	1265:1268	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	9	75	theme	cell	1736:1739	arg1	apoptosis					1741:1749	cell apoptosis	1736:1749	cell apoptosis	1736:1749	CONCLUSION AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.
31527444	8	76	theme	Bcl-2/Bax	1466:1474	arg1	ratio					1476:1480	raised Bcl-2/Bax ratio	1459:1480	raised Bcl-2/Bax ratio	1459:1480	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	2	77	theme	study	190:194	arg1	purpose					174:180	The purpose	170:180	The purpose of this study	170:194	The purpose of this study was to characterize the polysaccharides from Athyrium multidentatum (Doll.)
31527444	4	78	theme	nuclear	534:540	arg1	resonance					551:559	1H nuclear magnetic resonance	531:559	1H nuclear magnetic resonance (1H NMR) spectroscopy	531:581	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	4	78	theme	nuclear	534:540	arg1	NMR					565:567	1H NMR	562:567	1H NMR	562:567	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	6	79	theme	blotting	810:817	arg1	assays					819:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays	717:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays	717:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	8	80	theme	mRNA	1442:1445	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	81	theme	caspase-3	1497:1505	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	82	theme	nuclear	1336:1342	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	82	theme	nuclear	1336:1342	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	0	83	from	multidentatum	86:98	arg1	Doll					101:104	Doll	101:104	Doll	101:104	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	83	from	multidentatum	86:98	arg1	Polysaccharides					56:70	Polysaccharides	56:70	Polysaccharides from Athyrium multidentatum	56:98	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	83	from	multidentatum	86:98	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization	0:26	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	0	83	from	multidentatum	86:98	arg1	Activity					44:51	Antioxidant Activity	32:51	Antioxidant Activity	32:51	Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.)
31527444	8	84	theme	2-related	1361:1369	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	84	theme	2-related	1361:1369	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	6	85	theme	protein	864:870	arg1	expression					872:881	the gene and protein expression	851:881	expression	872:881	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	7	86	theme	Da	1185:1186	arg1	weight					1169:1174	a molecular weight	1157:1174	a molecular weight of 33203 Da (Mw)	1157:1191	KEY FINDINGS Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw).
31527444	8	87	theme	enhanced	1524:1531	arg1	Akt					1533:1535	enhanced Akt	1524:1535	enhanced Akt	1524:1535	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	4	88	theme	AMC	616:618	arg1	polysaccharides					620:634	AMC polysaccharides	616:634	AMC polysaccharides	616:634	METHODS A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides.
31527444	6	89	theme	reaction	779:786	arg1	assays					819:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays	717:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays	717:824	Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.
31527444	8	90	theme	Nrf2	1570:1573	arg1	Nrf2					1381:1384	Nrf2	1381:1384	Nrf2	1381:1384	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	ratio					1476:1480	raised Bcl-2/Bax ratio	1459:1480	raised Bcl-2/Bax ratio	1459:1480	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	PI3K					1324:1327	PI3K	1324:1327	PI3K	1324:1327	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	Akt					1533:1535	enhanced Akt	1524:1535	enhanced Akt	1524:1535	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	factor					1371:1376	nuclear factor-erythroid 2-related factor 2	1336:1378	nuclear factor-erythroid 2-related factor 2 (Nrf2)	1336:1385	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	expression					1447:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	hemeoxygenase-1 (HO-1) mRNA expression	1419:1456	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	phosphorylation					1538:1552	phosphorylation	1538:1552	phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression	1538:1601	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	expression					1641:1650	Bax protein expression	1629:1650	Bax protein expression	1629:1650	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	O3a					1401:1403	forkhead box O3a	1388:1403	forkhead box O3a (FOXO3a)	1388:1412	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	Akt					1331:1333	Akt	1331:1333	Akt	1331:1333	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	3-kinase					1314:1321	upregulated phosphatidylinositol 3-kinase	1281:1321	upregulated phosphatidylinositol 3-kinase (PI3K)	1281:1328	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	expression					1512:1521	downregulated caspase-3 mRNA expression	1483:1521	downregulated caspase-3 mRNA expression	1483:1521	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	caspase-3					1614:1622	decreased caspase-3	1604:1622	decreased caspase-3	1604:1622	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
31527444	8	90	theme	Nrf2	1570:1573	arg1	FOXO3a					1406:1411	FOXO3a	1406:1411	FOXO3a	1406:1411	AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.
30989978	6	0	from	protein	645:651	arg1	batches					681:687	71 batches	678:687	71 batches	678:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	11	1	theme	multiple	1237:1244	arg1	indicators					1246:1255	multiple indicators	1237:1255	multiple indicators	1237:1255	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	6	2	theme	zoning	704:709	arg1	quality					693:699	the quality	689:699	the quality of zoning	689:709	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	12	3	theme	eco-environmental	1319:1335	arg1	factors					1337:1343	the eco-environmental factors	1315:1343	the eco-environmental factors	1315:1343	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	10	4	from	content	1079:1085	arg1	Mongolia					1108:1115	Inner Mongolia	1102:1115	Inner Mongolia	1102:1115	The content of naringin in Inner Mongolia was relatively high.
30989978	4	5	theme	planted	503:509	arg1	barbarum					514:521	planted L. barbarum	503:521	planted L. barbarum	503:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	15	6	contain	have	1811:1814	arg2	cultivated					1846:1855	L. barbarum cultivated	1834:1855	L. barbarum cultivated	1834:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	15	6	contain	have	1811:1814	arg2	amount					1824:1829	a small amount	1816:1829	a small amount of L. barbarum cultivated	1816:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	15	6	contain	have	1811:1814	arg1	Liaoning					1778:1785	Liaoning	1778:1785	Liaoning	1778:1785	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	15	6	contain	have	1811:1814	arg1	province					1797:1804	province	1797:1804	province	1797:1804	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	12	7	with	composition	1462:1472	arg1	proportion					1483:1492	low proportion	1479:1492	low proportion	1479:1492	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	6	8	from	components	615:624	arg1	batches					681:687	71 batches	678:687	71 batches	678:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	13	9	theme	central	1648:1654	arg1	areas					1656:1660	the northwest and south central areas	1624:1660	the northwest and south central areas	1624:1660	The literatures such as " Chinese Flora" are mostly recorded that L. barbarum are planted in the northwest and south central areas.
30989978	2	10	theme	limited	118:124	arg1	resources					131:139	limited wild resources	118:139	limited wild resources	118:139	Due to limited wild resources,Lycii Fructus is mainly derived from the cultivation of Lycium barbarum when it circulate in the market.
30989978	12	11	theme	chemical	1291:1298	arg1	components					1300:1309	the four chemical components	1282:1309	the four chemical components	1282:1309	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	9	12	theme	protein	1027:1033	arg1	high					1069:1072	high	1069:1072	high	1069:1072	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	9	12	theme	protein	1027:1033	arg1	content					1035:1041	the protein content	1023:1041	the protein content of Ningxia	1023:1052	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	11	13	theme	different	1172:1180	arg1	areas					1192:1196	different producing areas	1172:1196	different producing areas	1172:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	3	14	from	quality	312:318	arg1	regions					352:358	different regions	342:358	different regions	342:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	16	15	theme	barbarum	1974:1981	arg1	cultivation					1956:1966	the cultivation	1952:1966	the cultivation of L. barbarum	1952:1981	The result shows that in the middle temperature zone and warm temperature zone there are also the cultivation of L. barbarum.
30989978	14	16	theme	barbarum	1708:1715	arg1	record					1675:1680	no record	1672:1680	no record of artificial planting L. barbarum in the northeast region	1672:1739	There is no record of artificial planting L. barbarum in the northeast region.
30989978	12	17	theme	chemical	1453:1460	arg1	composition					1462:1472	the chemical composition	1449:1472	the chemical composition with low proportion	1449:1492	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	7	18	from	contents	810:817	arg1	area					884:887	the sampling area	871:887	the sampling area	871:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	15	19	theme	cultivated	1846:1855	arg1	cultivated					1846:1855	L. barbarum cultivated	1834:1855	L. barbarum cultivated	1834:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	15	19	theme	cultivated	1846:1855	arg1	amount					1824:1829	a small amount	1816:1829	a small amount of L. barbarum cultivated	1816:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	7	20	theme	naringin	859:866	arg1	contents					810:817	the contents	806:817	the contents of polysaccharides,protein,vitamins and naringin in the sampling area	806:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	3	21	from	regions	352:358	arg1	quality					312:318	the quality	308:318	the quality of Lycii Fructus from different regions	308:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	3	21	from	regions	352:358	arg1	Fructus					329:335	Lycii Fructus	323:335	Lycii Fructus from different regions	323:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	7	22	theme	vitamins	846:853	arg1	contents					810:817	the contents	806:817	the contents of polysaccharides,protein,vitamins and naringin in the sampling area	806:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	13	23	theme	Flora	1565:1569	arg1	"					1570:1570	" Chinese Flora"	1555:1570	" Chinese Flora"	1555:1570	The literatures such as " Chinese Flora" are mostly recorded that L. barbarum are planted in the northwest and south central areas.
30989978	7	24	theme	protein	838:844	arg1	contents					810:817	the contents	806:817	the contents of polysaccharides,protein,vitamins and naringin in the sampling area	806:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	12	25	theme	percentage	1394:1403	arg1	content					1405:1411	percentage content	1394:1411	percentage content	1394:1411	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	12	26	with	composition	1358:1368	arg1	percentage					1380:1389	high percentage	1375:1389	high percentage of percentage content	1375:1411	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	7	27	theme	polysaccharides	822:836	arg1	contents					810:817	the contents	806:817	the contents of polysaccharides,protein,vitamins and naringin in the sampling area	806:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	1	28	theme	Lycii	53:57	arg1	Fructus					59:65	Lycii Fructus	53:65	Lycii Fructus	53:65	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	1	28	theme	Lycii	53:57	arg1	medicine					101:108	a commonly used Chinese herbal medicine	70:108	a commonly used Chinese herbal medicine	70:108	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	4	29	theme	county	406:411	arg1	areas					413:417	the county areas	402:417	the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum	402:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	11	30	theme	Lycii	1153:1157	arg1	Fructus					1159:1165	Lycii Fructus	1153:1165	Lycii Fructus from different producing areas	1153:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	6	31	theme	chemical	606:613	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	8	32	theme	vitamin	905:911	arg1	content					913:919	the vitamin content	901:919	the vitamin content in Inner Mongolia	901:937	Among them,the vitamin content in Inner Mongolia was relatively high.
30989978	8	32	theme	vitamin	905:911	arg1	high					954:957	high	954:957	high	954:957	Among them,the vitamin content in Inner Mongolia was relatively high.
30989978	13	33	theme	"	1555:1555	arg1	"					1570:1570	" Chinese Flora"	1555:1570	" Chinese Flora"	1555:1570	The literatures such as " Chinese Flora" are mostly recorded that L. barbarum are planted in the northwest and south central areas.
30989978	11	34	from	quality	1142:1148	arg1	areas					1192:1196	different producing areas	1172:1196	different producing areas	1172:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	2	35	attach	derived	165:171	arg1	cultivation					182:192	the cultivation	178:192	the cultivation of Lycium barbarum when it circulate in the market	178:243	Due to limited wild resources,Lycii Fructus is mainly derived from the cultivation of Lycium barbarum when it circulate in the market.
30989978	2	35	attach	derived	165:171	arg2	Fructus					147:153	Fructus	147:153	Fructus	147:153	Due to limited wild resources,Lycii Fructus is mainly derived from the cultivation of Lycium barbarum when it circulate in the market.
30989978	3	36	theme	Lycii	323:327	arg1	Fructus					329:335	Lycii Fructus	323:335	Lycii Fructus from different regions	323:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	4	37	theme	Mongolia	463:470	arg1	areas					413:417	the county areas	402:417	the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum	402:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	15	38	theme	L.	1834:1835	arg1	cultivated					1846:1855	L. barbarum cultivated	1834:1855	L. barbarum cultivated	1834:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	0	39	theme	quality	10:16	arg1	regionalization					18:32	quality regionalization	10:32	quality regionalization of Lycii Fructus	10:49	[Study on quality regionalization of Lycii Fructus].
30989978	12	40	theme	high	1375:1378	arg1	percentage					1380:1389	high percentage	1375:1389	high percentage of percentage content	1375:1411	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	1	41	theme	Chinese	86:92	arg1	Fructus					59:65	Lycii Fructus	53:65	Lycii Fructus	53:65	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	1	41	theme	Chinese	86:92	arg1	medicine					101:108	a commonly used Chinese herbal medicine	70:108	a commonly used Chinese herbal medicine	70:108	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	12	42	theme	soil	1517:1520	arg1	factors					1522:1528	soil factors	1517:1528	soil factors	1517:1528	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	9	43	from	content	964:970	arg1	Xingjiang					993:1001	Xingjiang	993:1001	Xingjiang	993:1001	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	6	44	theme	Arc	735:737	arg1	GIS					739:741	Arc GIS	735:741	Arc GIS	735:741	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	6	45	from	batches	681:687	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	0	46	theme	Fructus	43:49	arg1	regionalization					18:32	quality regionalization	10:32	quality regionalization of Lycii Fructus	10:49	[Study on quality regionalization of Lycii Fructus].
30989978	9	47	theme	polysaccharide	975:988	arg1	high					1018:1021	high	1018:1021	high	1018:1021	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	9	47	theme	polysaccharide	975:988	arg1	content					964:970	The content	960:970	The content of polysaccharide in Xingjiang	960:1001	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	4	48	theme	L.	511:512	arg1	barbarum					514:521	planted L. barbarum	503:521	planted L. barbarum	503:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	11	49	theme	indicators	1246:1255	arg1	evaluation					1223:1232	a comprehensive evaluation	1207:1232	a comprehensive evaluation of multiple indicators	1207:1255	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	12	50	theme	factors	1337:1343	arg1	analysis					1270:1277	the analysis	1266:1277	the analysis of the four chemical components and the eco-environmental factors	1266:1343	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	16	51	theme	temperature	1894:1904	arg1	zone					1906:1909	middle temperature zone	1887:1909	middle temperature zone	1887:1909	The result shows that in the middle temperature zone and warm temperature zone there are also the cultivation of L. barbarum.
30989978	13	52	theme	northwest	1628:1636	arg1	areas					1656:1660	the northwest and south central areas	1624:1660	the northwest and south central areas	1624:1660	The literatures such as " Chinese Flora" are mostly recorded that L. barbarum are planted in the northwest and south central areas.
30989978	5	53	theme	samples	564:570	arg1	batches					553:559	71 batches	550:559	71 batches of samples	550:570	The county areas obtained 71 batches of samples.
30989978	2	54	theme	wild	126:129	arg1	resources					131:139	limited wild resources	118:139	limited wild resources	118:139	Due to limited wild resources,Lycii Fructus is mainly derived from the cultivation of Lycium barbarum when it circulate in the market.
30989978	5	55	theme	county	528:533	arg1	areas					535:539	The county areas	524:539	The county areas	524:539	The county areas obtained 71 batches of samples.
30989978	9	56	theme	Ningxia	1046:1052	arg1	high					1069:1072	high	1069:1072	high	1069:1072	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	9	56	theme	Ningxia	1046:1052	arg1	content					1035:1041	the protein content	1023:1041	the protein content of Ningxia	1023:1052	The content of polysaccharide in Xingjiang was relatively high,the protein content of Ningxia was relatively high.
30989978	12	57	theme	components	1300:1309	arg1	analysis					1270:1277	the analysis	1266:1277	the analysis of the four chemical components and the eco-environmental factors	1266:1343	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	11	58	theme	producing	1182:1190	arg1	areas					1192:1196	different producing areas	1172:1196	different producing areas	1172:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	12	59	theme	low	1479:1481	arg1	proportion					1483:1492	low proportion	1479:1492	low proportion	1479:1492	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	3	60	theme	Related	246:252	arg1	studies					254:260	Related studies	246:260	Related studies	246:260	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	11	61	theme	comprehensive	1209:1221	arg1	evaluation					1223:1232	a comprehensive evaluation	1207:1232	a comprehensive evaluation of multiple indicators	1207:1255	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	2	62	theme	barbarum	204:211	arg1	cultivation					182:192	the cultivation	178:192	the cultivation of Lycium barbarum when it circulate in the market	178:243	Due to limited wild resources,Lycii Fructus is mainly derived from the cultivation of Lycium barbarum when it circulate in the market.
30989978	14	63	theme	northeast	1724:1732	arg1	region					1734:1739	the northeast region	1720:1739	the northeast region	1720:1739	There is no record of artificial planting L. barbarum in the northeast region.
30989978	1	64	theme	herbal	94:99	arg1	Fructus					59:65	Lycii Fructus	53:65	Lycii Fructus	53:65	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	1	64	theme	herbal	94:99	arg1	medicine					101:108	a commonly used Chinese herbal medicine	70:108	a commonly used Chinese herbal medicine	70:108	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	8	65	from	content	913:919	arg1	Mongolia					930:937	Inner Mongolia	924:937	Inner Mongolia	924:937	Among them,the vitamin content in Inner Mongolia was relatively high.
30989978	10	66	theme	naringin	1090:1097	arg1	content					1079:1085	The content	1075:1085	The content of naringin in Inner Mongolia	1075:1115	The content of naringin in Inner Mongolia was relatively high.
30989978	10	66	theme	naringin	1090:1097	arg1	high					1132:1135	high	1132:1135	high	1132:1135	The content of naringin in Inner Mongolia was relatively high.
30989978	7	67	theme	sampling	875:882	arg1	area					884:887	the sampling area	871:887	the sampling area	871:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	12	68	theme	climatic	1428:1435	arg1	factors					1437:1443	climatic factors	1428:1443	climatic factors	1428:1443	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	4	69	theme	research	380:387	arg1	team					389:392	our research team	376:392	our research team	376:392	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	3	70	from	differences	293:303	arg1	quality					312:318	the quality	308:318	the quality of Lycii Fructus from different regions	308:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	4	71	theme	Gansu	430:434	arg1	areas					413:417	the county areas	402:417	the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum	402:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	12	72	theme	content	1405:1411	arg1	percentage					1380:1389	high percentage	1375:1389	high percentage of percentage content	1375:1411	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	16	73	theme	middle	1887:1892	arg1	zone					1906:1909	middle temperature zone	1887:1909	middle temperature zone	1887:1909	The result shows that in the middle temperature zone and warm temperature zone there are also the cultivation of L. barbarum.
30989978	10	74	theme	Inner	1102:1106	arg1	Mongolia					1108:1115	Inner Mongolia	1102:1115	Inner Mongolia	1102:1115	The content of naringin in Inner Mongolia was relatively high.
30989978	6	75	theme	components	615:624	arg1	content					590:596	the content	586:596	the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	586:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	4	76	theme	Ningxia	422:428	arg1	areas					413:417	the county areas	402:417	the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum	402:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	11	77	theme	Fructus	1159:1165	arg1	quality					1142:1148	The quality	1138:1148	The quality of Lycii Fructus from different producing areas	1138:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	11	78	from	areas	1192:1196	arg1	quality					1142:1148	The quality	1138:1148	The quality of Lycii Fructus from different producing areas	1138:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	11	78	from	areas	1192:1196	arg1	Fructus					1159:1165	Lycii Fructus	1153:1165	Lycii Fructus from different producing areas	1153:1196	The quality of Lycii Fructus from different producing areas requires a comprehensive evaluation of multiple indicators.
30989978	8	79	theme	Inner	924:928	arg1	Mongolia					930:937	Inner Mongolia	924:937	Inner Mongolia	924:937	Among them,the vitamin content in Inner Mongolia was relatively high.
30989978	3	80	theme	different	342:350	arg1	regions					352:358	different regions	342:358	different regions	342:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	13	81	theme	Chinese	1557:1563	arg1	"					1570:1570	" Chinese Flora"	1555:1570	" Chinese Flora"	1555:1570	The literatures such as " Chinese Flora" are mostly recorded that L. barbarum are planted in the northwest and south central areas.
30989978	16	82	theme	temperature	1920:1930	arg1	zone					1932:1935	warm temperature zone	1915:1935	warm temperature zone	1915:1935	The result shows that in the middle temperature zone and warm temperature zone there are also the cultivation of L. barbarum.
30989978	1	83	theme	used	81:84	arg1	Fructus					59:65	Lycii Fructus	53:65	Lycii Fructus	53:65	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	1	83	theme	used	81:84	arg1	medicine					101:108	a commonly used Chinese herbal medicine	70:108	a commonly used Chinese herbal medicine	70:108	Lycii Fructus is a commonly used Chinese herbal medicine.
30989978	3	84	theme	Fructus	329:335	arg1	quality					312:318	the quality	308:318	the quality of Lycii Fructus from different regions	308:358	Related studies have shown that there are some differences in the quality of Lycii Fructus from different regions.
30989978	15	85	theme	barbarum	1837:1844	arg1	cultivated					1846:1855	L. barbarum cultivated	1834:1855	L. barbarum cultivated	1834:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	7	86	from	differences	791:801	arg1	contents					810:817	the contents	806:817	the contents of polysaccharides,protein,vitamins and naringin in the sampling area	806:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	4	87	theme	Xinjiang	444:451	arg1	areas					413:417	the county areas	402:417	the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum	402:521	In August 2017,our research team went to the county areas of Ningxia,Gansu,Qinghai,Xinjiang and Inner Mongolia,which included 13 counties and planted L. barbarum.
30989978	6	88	theme	naringin	666:673	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	6	89	from	vitamins	653:660	arg1	batches					681:687	71 batches	678:687	71 batches	678:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	15	90	theme	small	1818:1822	arg1	cultivated					1846:1855	L. barbarum cultivated	1834:1855	L. barbarum cultivated	1834:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	15	90	theme	small	1818:1822	arg1	amount					1824:1829	a small amount	1816:1829	a small amount of L. barbarum cultivated	1816:1855	Through this study,it is found that Liaoning and Jilin province also have a small amount of L. barbarum cultivated.
30989978	16	91	theme	warm	1915:1918	arg1	zone					1932:1935	warm temperature zone	1915:1935	warm temperature zone	1915:1935	The result shows that in the middle temperature zone and warm temperature zone there are also the cultivation of L. barbarum.
30989978	6	92	theme	vitamins	653:660	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	7	93	theme	significant	779:789	arg1	differences					791:801	significant differences	779:801	significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area	779:887	The results showed that there were significant differences in the contents of polysaccharides,protein,vitamins and naringin in the sampling area.
30989978	6	94	from	naringin	666:673	arg1	batches					681:687	71 batches	678:687	71 batches	678:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	6	95	theme	protein	645:651	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	12	96	theme	chemical	1349:1356	arg1	composition					1358:1368	the chemical composition	1345:1368	the chemical composition with high percentage of percentage content	1345:1411	Through the analysis of the four chemical components and the eco-environmental factors,the chemical composition with high percentage of percentage content is affected by climatic factors,and the chemical composition with low proportion is greatly affected by soil factors.
30989978	6	97	from	polysaccharides	629:643	arg1	batches					681:687	71 batches	678:687	71 batches	678:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	6	98	theme	polysaccharides	629:643	arg1	components					615:624	four chemical components	601:624	four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches	601:687	According to the content of four chemical components of polysaccharides,protein,vitamins and naringin in 71 batches,the quality of zoning was studied by SPSS and Arc GIS.
30989978	14	99	from	record	1675:1680	arg1	region					1734:1739	the northeast region	1720:1739	the northeast region	1720:1739	There is no record of artificial planting L. barbarum in the northeast region.
29616312	8	0	theme	following	1108:1116	arg1	composition					1124:1134	the following sugar composition	1104:1134	the following sugar composition (% w/w)	1104:1142	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	8	0	theme	following	1108:1116	arg1	w/w					1139:1141	% w/w	1137:1141	% w/w	1137:1141	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	12	1	theme	EPS	1709:1711	arg1	fraction					1713:1720	EPS fraction	1709:1720	EPS fraction	1709:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	11	2	theme	due	1557:1559	arg1	hydrophilicity					1541:1554	an extremely high hydrophilicity	1523:1554	an extremely high hydrophilicity	1523:1554	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	5	3	theme	extracellular	707:719	arg1	synthesis					729:737	extracellular polymer synthesis	707:737	extracellular polymer synthesis	707:737	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	3	4	from	industry	538:545	arg1	by-product					512:521	a by-product	510:521	a by-product from the dairy industry	510:545	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	12	5	theme	glucosamine	1668:1678	arg1	presence					1656:1663	the presence	1652:1663	the presence of glucosamine in the highest proportion in EPS fraction	1652:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	5	theme	glucosamine	1668:1678	arg1	analysis					1610:1617	The analysis	1606:1617	The analysis of its functional properties	1606:1646	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	11	6	theme	PGA	1593:1595	arg1	presence					1581:1588	the simultaneous presence	1564:1588	the simultaneous presence of PGA and EPS	1564:1603	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	5	7	theme	synthesis	729:737	arg1	level					698:702	The highest level	686:702	The highest level of extracellular polymer synthesis by C. canadensis 28	686:757	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	8	located	observed	763:770	arg1	concentration					798:810	an unusually high NaCl concentration	775:810	an unusually high NaCl concentration (15% w/v)	775:820	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	8	located	observed	763:770	arg2	level					698:702	The highest level	686:702	The highest level of extracellular polymer synthesis by C. canadensis 28	686:757	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	8	located	observed	763:770	arg1	w/v					817:819	15% w/v	813:819	15% w/v	813:819	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	8	9	theme	%	1137:1137	arg1	composition					1124:1134	the following sugar composition	1104:1134	the following sugar composition (% w/w)	1104:1142	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	8	9	theme	%	1137:1137	arg1	w/w					1139:1141	% w/w	1137:1141	% w/w	1137:1141	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	10	10	theme	PGA	1476:1478	arg1	fraction					1480:1487	PGA fraction	1476:1487	PGA fraction	1476:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	7	11	theme	w/w	1067:1069	arg1	%					1065:1065	75.7% w/w	1061:1069	75.7% w/w	1061:1069	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	7	11	theme	w/w	1067:1069	arg1	acid					1049:1052	polyglutamic acid	1036:1052	polyglutamic acid (PGA) (75.7% w/w)	1036:1070	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	1	12	theme	promising	193:201	arg1	perspectives					203:214	promising perspectives	193:214	promising perspectives for their biotechnological exploration	193:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	5	13	theme	C.	742:743	arg1	canadensis					745:754	C. canadensis 28	742:757	C. canadensis 28	742:757	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	2	14	theme	polymer	372:378	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	14	theme	polymer	372:378	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	9	15	theme	foaming	1369:1375	arg1	ability					1377:1383	good foaming ability	1364:1383	good foaming ability	1364:1383	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	6	16	theme	polymer	857:863	arg1	analysis					832:839	Chemical analysis	823:839	Chemical analysis of the purified polymer	823:863	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	1	17	theme	biotechnological	226:241	arg1	exploration					243:253	their biotechnological exploration	220:253	their biotechnological exploration	220:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	2	18	theme	bacterium	276:284	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	18	theme	bacterium	276:284	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	7	19	theme	polyglutamic	1036:1047	arg1	%					1065:1065	75.7% w/w	1061:1069	75.7% w/w	1061:1069	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	7	19	theme	polyglutamic	1036:1047	arg1	PGA					1055:1057	PGA	1055:1057	PGA	1055:1057	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	7	19	theme	polyglutamic	1036:1047	arg1	acid					1049:1052	polyglutamic acid	1036:1052	polyglutamic acid (PGA) (75.7% w/w)	1036:1070	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	2	20	theme	Moderate	256:263	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	20	theme	Moderate	256:263	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	7	21	theme	protein	991:997	arg1	fraction					999:1006	the protein fraction	987:1006	the protein fraction	987:1006	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	6	22	theme	protein	942:948	arg1	fraction					950:957	protein fraction	942:957	protein fraction (72% w/w)	942:967	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	6	22	theme	protein	942:948	arg1	%					962:962	72% w/w	960:966	72% w/w	960:966	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	12	23	from	analysis	1610:1617	arg1	proportion					1695:1704	the highest proportion	1683:1704	the highest proportion in EPS fraction	1683:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	24	theme	properties	1637:1646	arg1	presence					1656:1663	the presence	1652:1663	the presence of glucosamine in the highest proportion in EPS fraction	1652:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	24	theme	properties	1637:1646	arg1	analysis					1610:1617	The analysis	1606:1617	The analysis of its functional properties	1606:1646	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	2	25	attach	isolated	321:328	arg1	salterns					343:350	salterns	343:350	salterns	343:350	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	25	attach	isolated	321:328	arg2	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	25	attach	isolated	321:328	arg2	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	0	26	theme	Chromohalobacter	70:85	arg1	canadensis					87:96	a halophilic bacterium Chromohalobacter canadensis 28	47:99	a halophilic bacterium Chromohalobacter canadensis 28	47:99	Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.
29616312	7	27	theme	HPLC	970:973	arg1	analysis					975:982	HPLC analysis	970:982	HPLC analysis of the protein fraction	970:1006	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	1	28	theme	compounds	158:166	arg1	lot					147:149	a lot	145:149	a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration	145:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	1	28	theme	compounds	158:166	arg1	compounds					158:166	new compounds	154:166	new compounds whose properties suggest promising perspectives for their biotechnological exploration	154:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	3	29	theme	best	409:412	arg1	source					421:426	The best carbon source	405:426	The best carbon source for extracellular polymer production	405:463	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	3	29	theme	best	409:412	arg1	lactose					481:487	lactose	481:487	lactose	481:487	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	12	30	attach	presence	1656:1663	arg2	properties					1637:1646	its functional properties	1622:1646	its functional properties	1622:1646	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	30	attach	presence	1656:1663	arg1	proportion					1695:1704	the highest proportion	1683:1704	the highest proportion in EPS fraction	1683:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	30	attach	presence	1656:1663	arg2	glucosamine					1668:1678	glucosamine	1668:1678	glucosamine	1668:1678	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	5	31	theme	high	788:791	arg1	concentration					798:810	an unusually high NaCl concentration	775:810	an unusually high NaCl concentration (15% w/v)	775:820	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	31	theme	high	788:791	arg1	w/v					817:819	15% w/v	813:819	15% w/v	813:819	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	12	32	from	application	1796:1806	arg1	industry					1825:1832	the cosmetics industry	1811:1832	the cosmetics industry	1811:1832	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	10	33	contain	containing	1465:1474	arg1	polymer					1457:1463	a polymer	1455:1463	a polymer containing PGA fraction	1455:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	10	33	contain	containing	1465:1474	arg2	fraction					1480:1487	PGA fraction	1476:1487	PGA fraction	1476:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	3	34	theme	extracellular	432:444	arg1	production					454:463	extracellular polymer production	432:463	extracellular polymer production	432:463	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	0	35	theme	Extracellular	0:12	arg1	substance					22:30	Extracellular polymer substance	0:30	Extracellular polymer substance	0:30	Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.
29616312	12	36	theme	C.	1775:1776	arg1	canadensis					1778:1787	C. canadensis 28	1775:1790	C. canadensis 28	1775:1790	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	12	37	from	presence	1656:1663	arg1	proportion					1695:1704	the highest proportion	1683:1704	the highest proportion in EPS fraction	1683:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	10	38	theme	first	1398:1402	arg1	report					1404:1409	the first report	1394:1409	the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction	1394:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	10	38	theme	first	1398:1402	arg1	This					1386:1389	This	1386:1389	This	1386:1389	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	6	39	theme	exopolysaccharide	893:909	arg1	fraction					917:924	an exopolysaccharide (EPS) fraction	890:924	an exopolysaccharide (EPS) fraction (14.3% w/w)	890:936	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	6	39	theme	exopolysaccharide	893:909	arg1	%					931:931	14.3% w/w	927:935	14.3% w/w	927:935	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	0	40	theme	halophilic	49:58	arg1	canadensis					87:96	a halophilic bacterium Chromohalobacter canadensis 28	47:99	a halophilic bacterium Chromohalobacter canadensis 28	47:99	Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.
29616312	9	41	theme	high	1286:1289	arg1	behavior					1300:1307	high swelling behavior	1286:1307	high swelling behavior	1286:1307	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	4	42	theme	culture	574:580	arg1	medium					582:587	the culture medium	570:587	the culture medium	570:587	After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold.
29616312	2	43	theme	EP	391:392	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	43	theme	EP	391:392	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	8	44	theme	EPS	1073:1075	arg1	analysis					1086:1093	EPS fraction analysis	1073:1093	EPS fraction analysis	1073:1093	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	11	45	theme	simultaneous	1568:1579	arg1	presence					1581:1588	the simultaneous presence	1564:1588	the simultaneous presence of PGA and EPS	1564:1603	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	11	46	theme	high	1536:1539	arg1	hydrophilicity					1541:1554	an extremely high hydrophilicity	1523:1554	an extremely high hydrophilicity	1523:1554	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	6	47	theme	w/w	933:935	arg1	fraction					917:924	an exopolysaccharide (EPS) fraction	890:924	an exopolysaccharide (EPS) fraction (14.3% w/w)	890:936	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	6	47	theme	w/w	933:935	arg1	%					931:931	14.3% w/w	927:935	14.3% w/w	927:935	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	12	48	from	proportion	1695:1704	arg1	fraction					1713:1720	EPS fraction	1709:1720	EPS fraction	1709:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	5	49	theme	polymer	721:727	arg1	synthesis					729:737	extracellular polymer synthesis	707:737	extracellular polymer synthesis	707:737	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	6	50	theme	purified	848:855	arg1	polymer					857:863	the purified polymer	844:863	the purified polymer	844:863	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	9	51	theme	stabilizing	1336:1346	arg1	properties					1348:1357	stabilizing properties	1336:1357	stabilizing properties	1336:1357	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	8	52	theme	sugar	1118:1122	arg1	composition					1124:1134	the following sugar composition	1104:1134	the following sugar composition (% w/w)	1104:1142	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	8	52	theme	sugar	1118:1122	arg1	w/w					1139:1141	% w/w	1137:1141	% w/w	1137:1141	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	12	53	theme	highest	1687:1693	arg1	proportion					1695:1704	the highest proportion	1683:1704	the highest proportion in EPS fraction	1683:1720	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	4	54	theme	polymer	637:643	arg1	biosynthesis					645:656	polymer biosynthesis	637:656	polymer biosynthesis	637:656	After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold.
29616312	6	55	theme	fraction	917:924	arg1	presence					878:885	the presence	874:885	the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w)	874:967	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	5	56	theme	highest	690:696	arg1	level					698:702	The highest level	686:702	The highest level of extracellular polymer synthesis by C. canadensis 28	686:757	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	6	57	theme	Chemical	823:830	arg1	analysis					832:839	Chemical analysis	823:839	Chemical analysis of the purified polymer	823:863	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	2	58	theme	substance	380:388	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	58	theme	substance	380:388	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	9	59	theme	good	1364:1367	arg1	ability					1377:1383	good foaming ability	1364:1383	good foaming ability	1364:1383	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	2	60	theme	extracellular	358:370	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	60	theme	extracellular	358:370	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	6	61	theme	w/w	964:966	arg1	fraction					950:957	protein fraction	942:957	protein fraction (72% w/w)	942:967	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	6	61	theme	w/w	964:966	arg1	%					962:962	72% w/w	960:966	72% w/w	960:966	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	7	62	theme	acid	1049:1052	arg1	presence					1024:1031	the main presence	1015:1031	the main presence of polyglutamic acid (PGA) (75.7% w/w)	1015:1070	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	2	63	theme	Chromohalobacter	286:301	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	63	theme	Chromohalobacter	286:301	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	64	theme	halophilic	265:274	arg1	producer					395:402	an extracellular polymer substance (EP) producer	355:402	an extracellular polymer substance (EP) producer	355:402	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	2	64	theme	halophilic	265:274	arg1	canadensis					303:312	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28	256:315	Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer.
29616312	6	65	theme	fraction	950:957	arg1	presence					878:885	the presence	874:885	the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w)	874:967	Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w).
29616312	7	66	theme	main	1019:1022	arg1	presence					1024:1031	the main presence	1015:1031	the main presence of polyglutamic acid (PGA) (75.7% w/w)	1015:1070	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	7	67	theme	fraction	999:1006	arg1	analysis					975:982	HPLC analysis	970:982	HPLC analysis of the protein fraction	970:1006	HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w).
29616312	1	68	theme	lot	147:149	arg1	microorganisms					113:126	Halophilic microorganisms	102:126	Halophilic microorganisms	102:126	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	1	68	theme	lot	147:149	arg1	producers					132:140	producers	132:140	producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration	132:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	1	69	theme	new	154:156	arg1	compounds					158:166	new compounds	154:166	new compounds whose properties suggest promising perspectives for their biotechnological exploration	154:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	12	70	theme	functional	1626:1635	arg1	properties					1637:1646	its functional properties	1622:1646	its functional properties	1622:1646	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	5	71	theme	%	815:815	arg1	concentration					798:810	an unusually high NaCl concentration	775:810	an unusually high NaCl concentration (15% w/v)	775:820	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	71	theme	%	815:815	arg1	w/v					817:819	15% w/v	813:819	15% w/v	813:819	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	3	72	theme	carbon	414:419	arg1	source					421:426	The best carbon source	405:426	The best carbon source for extracellular polymer production	405:463	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	3	72	theme	carbon	414:419	arg1	lactose					481:487	lactose	481:487	lactose	481:487	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	5	73	theme	NaCl	793:796	arg1	concentration					798:810	an unusually high NaCl concentration	775:810	an unusually high NaCl concentration (15% w/v)	775:820	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	5	73	theme	NaCl	793:796	arg1	w/v					817:819	15% w/v	813:819	15% w/v	813:819	The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v).
29616312	0	74	theme	polymer	14:20	arg1	substance					22:30	Extracellular polymer substance	0:30	Extracellular polymer substance	0:30	Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.
29616312	12	75	theme	cosmetics	1815:1823	arg1	industry					1825:1832	the cosmetics industry	1811:1832	the cosmetics industry	1811:1832	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	1	76	theme	Halophilic	102:111	arg1	microorganisms					113:126	Halophilic microorganisms	102:126	Halophilic microorganisms	102:126	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	1	76	theme	Halophilic	102:111	arg1	producers					132:140	producers	132:140	producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration	132:253	Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration.
29616312	3	77	theme	polymer	446:452	arg1	production					454:463	extracellular polymer production	432:463	extracellular polymer production	432:463	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	4	78	theme	physicochemical	593:607	arg1	conditions					609:618	physicochemical conditions	593:618	physicochemical conditions for cultivation	593:634	After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold.
29616312	9	79	theme	PGA	1257:1259	arg1	fractions					1269:1277	PGA and EPS fractions	1257:1277	fractions	1269:1277	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	9	80	theme	EPS	1265:1267	arg1	fractions					1269:1277	PGA and EPS fractions	1257:1277	fractions	1269:1277	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	11	81	theme	EPS	1601:1603	arg1	presence					1581:1588	the simultaneous presence	1564:1588	the simultaneous presence of PGA and EPS	1564:1603	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	0	82	theme	bacterium	60:68	arg1	canadensis					87:96	a halophilic bacterium Chromohalobacter canadensis 28	47:99	a halophilic bacterium Chromohalobacter canadensis 28	47:99	Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.
29616312	12	83	theme	EP	1757:1758	arg1	potential					1744:1752	the potential	1740:1752	the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry	1740:1832	The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.
29616312	4	84	theme	medium	582:587	arg1	conditions					609:618	physicochemical conditions	593:618	physicochemical conditions for cultivation	593:634	After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold.
29616312	4	84	theme	medium	582:587	arg1	optimization					554:565	optimization	554:565	optimization of the culture medium	554:587	After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold.
29616312	10	85	theme	able	1436:1439	arg1	bacterium					1426:1434	halophilic bacterium	1415:1434	halophilic bacterium able to synthesize a polymer containing PGA fraction	1415:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
29616312	11	86	theme	synthesized	1494:1504	arg1	biopolymer					1506:1515	The synthesized biopolymer	1490:1515	The synthesized biopolymer	1490:1515	The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS.
29616312	3	87	theme	dairy	532:536	arg1	industry					538:545	the dairy industry	528:545	the dairy industry	528:545	The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry.
29616312	9	88	theme	swelling	1291:1298	arg1	behavior					1300:1307	high swelling behavior	1286:1307	high swelling behavior	1286:1307	The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability.
29616312	8	89	theme	fraction	1077:1084	arg1	analysis					1086:1093	EPS fraction analysis	1073:1093	EPS fraction analysis	1073:1093	EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9.
29616312	10	90	theme	halophilic	1415:1424	arg1	bacterium					1426:1434	halophilic bacterium	1415:1434	halophilic bacterium able to synthesize a polymer containing PGA fraction	1415:1487	This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction.
31751721	8	0	theme	time	952:955	arg1	conditions					909:918	different conditions	899:918	different conditions of temperature, pH, and heating time	899:955	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	3	1	theme	α-lactalbumin	143:155	arg1	conjugates					116:125	conjugates	116:125	conjugates of tara gum and α-lactalbumin	116:155	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	7	2	theme	%	735:735	arg1	p < 0.05					742:749	p < 0.05	742:749	p < 0.05	742:749	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	7	2	theme	%	735:735	arg1	FAG					737:739	% FAG	735:739	% FAG (p < 0.05)	735:750	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	3	3	theme	heat-gelation	179:191	arg1	process					193:199	the heat-gelation process	175:199	the heat-gelation process with pH adjustment	175:218	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	8	4	theme	lower	863:867	arg1	sizes					857:861	average sizes	849:861	average sizes lower than 300 nm	849:879	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	7	5	theme	FAG	737:739	arg1	values					718:723	different values	708:723	different values of BI and % FAG (p < 0.05)	708:750	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	10	6	theme	tara	1258:1261	arg1	conjugates					1244:1253	conjugates	1244:1253	conjugates of tara gum and α-lactalbumin	1244:1283	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	5	7	theme	circular	529:536	arg1	dichroism					538:546	circular dichroism	529:546	circular dichroism	529:546	Nanostructured systems were characterized by dynamic light scattering, ζ-potential, circular dichroism, and intrinsic fluorescence to evaluate the structures.
31751721	7	8	theme	BI	728:729	arg1	values					718:723	different values	708:723	different values of BI and % FAG (p < 0.05)	708:750	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	8	9	theme	heating	944:950	arg1	time					952:955	heating time	944:955	heating time	944:955	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	9	10	theme	spray-dried	1113:1123	arg1	systems					1125:1131	spray-dried systems	1113:1131	spray-dried systems	1113:1131	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	9	11	theme	α-helix	1094:1100	arg1	content					1102:1108	the α-helix content	1090:1108	the α-helix content in spray-dried systems	1090:1131	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	4	12	theme	lyophilized	303:313	arg1	mixtures					330:337	lyophilized or spray-dried mixtures	303:337	lyophilized or spray-dried mixtures of TG and α-la	303:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	3	13	dep	tara	130:133	arg1	gum					135:137	gum	135:137	gum	135:137	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	5	14	theme	Nanostructured	445:458	arg1	systems					460:466	Nanostructured systems	445:466	Nanostructured systems	445:466	Nanostructured systems were characterized by dynamic light scattering, ζ-potential, circular dichroism, and intrinsic fluorescence to evaluate the structures.
31751721	7	15	from	intense	796:802	arg1	mixtures					819:826	lyophilized mixtures	807:826	lyophilized mixtures	807:826	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	4	16	theme	browning	380:387	arg1	BI					396:397	BI	396:397	BI	396:397	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	16	theme	browning	380:387	arg1	index					389:393	browning index	380:393	browning index (BI)	380:398	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	17	theme	spray-dried	318:328	arg1	mixtures					330:337	lyophilized or spray-dried mixtures	303:337	lyophilized or spray-dried mixtures of TG and α-la	303:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	0	18	theme	Nanostructured	0:13	arg1	conjugates					15:24	Nanostructured conjugates	0:24	Nanostructured conjugates from tara gum and α-lactalbumin.	0:57	Nanostructured conjugates from tara gum and α-lactalbumin.
31751721	2	19	theme	Structural	67:76	arg1	characterization					78:93	Structural characterization	67:93	Structural characterization.	67:94	Structural characterization.
31751721	10	20	dep	tara	1258:1261	arg1	tara					1258:1261	tara gum and α-lactalbumin	1258:1283	tara gum and α-lactalbumin	1258:1283	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	10	20	dep	tara	1258:1261	arg1	α-lactalbumin					1271:1283	α-lactalbumin	1271:1283	α-lactalbumin	1271:1283	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	10	20	dep	tara	1258:1261	arg1	gum					1263:1265	gum	1263:1265	gum	1263:1265	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	7	21	theme	lyophilized	677:687	arg1	mixtures					689:696	The spray-dried and lyophilized mixtures	657:696	The spray-dried and lyophilized mixtures	657:696	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	4	22	theme	dry-heating	281:291	arg1	method					293:298	the dry-heating method	277:298	the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la	277:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	9	23	theme	circular	970:977	arg1	dichroism					979:987	circular dichroism	970:987	circular dichroism	970:987	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	8	24	with	Nanostructures	829:842	arg1	sizes					857:861	average sizes	849:861	average sizes lower than 300 nm	849:879	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	3	25	theme	pH	206:207	arg1	adjustment					209:218	pH adjustment	206:218	pH adjustment	206:218	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	3	26	from	conjugates	116:125	arg1	Nanostructures					96:109	Nanostructures	96:109	Nanostructures from conjugates of tara gum and α-lactalbumin	96:155	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	9	27	theme	dichroism	979:987	arg1	Analyses					958:965	Analyses	958:965	Analyses of circular dichroism and intrinsic fluorescence	958:1014	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	7	28	theme	lyophilized	807:817	arg1	mixtures					819:826	lyophilized mixtures	807:826	lyophilized mixtures	807:826	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	4	29	from	method	293:298	arg1	mixtures					330:337	lyophilized or spray-dried mixtures	303:337	lyophilized or spray-dried mixtures of TG and α-la	303:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	5	30	theme	dynamic	490:496	arg1	scattering					504:513	dynamic light scattering	490:513	dynamic light scattering	490:513	Nanostructured systems were characterized by dynamic light scattering, ζ-potential, circular dichroism, and intrinsic fluorescence to evaluate the structures.
31751721	4	31	theme	%	437:437	arg1	groups					429:434	free amino groups	418:434	free amino groups (% FAG)	418:442	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	31	theme	%	437:437	arg1	FAG					439:441	% FAG	437:441	% FAG	437:441	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	9	32	theme	conformational	1023:1036	arg1	changes					1038:1044	conformational changes	1023:1044	conformational changes	1023:1044	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	9	33	theme	intrinsic	993:1001	arg1	fluorescence					1003:1014	intrinsic fluorescence	993:1014	intrinsic fluorescence	993:1014	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	7	34	from	mixtures	819:826	arg1	glycosylation					773:785	the glycosylation	769:785	the glycosylation	769:785	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	7	34	from	mixtures	819:826	arg1	intense					796:802	intense	796:802	intense	796:802	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	3	35	theme	tara	130:133	arg1	conjugates					116:125	conjugates	116:125	conjugates of tara gum and α-lactalbumin	116:155	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	9	36	theme	fluorescence	1003:1014	arg1	Analyses					958:965	Analyses	958:965	Analyses of circular dichroism and intrinsic fluorescence	958:1014	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	10	37	theme	studied	1171:1177	arg1	systems					1179:1185	the studied systems	1167:1185	the studied systems	1167:1185	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	7	38	theme	different	708:716	arg1	values					718:723	different values	708:723	different values of BI and % FAG (p < 0.05)	708:750	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	9	39	from	content	1102:1108	arg1	systems					1125:1131	spray-dried systems	1113:1131	spray-dried systems	1113:1131	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	8	40	theme	temperature	923:933	arg1	conditions					909:918	different conditions	899:918	different conditions of temperature, pH, and heating time	899:955	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	4	41	theme	amino	423:427	arg1	groups					429:434	free amino groups	418:434	free amino groups (% FAG)	418:442	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	41	theme	amino	423:427	arg1	FAG					439:441	% FAG	437:441	% FAG	437:441	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	8	42	theme	different	899:907	arg1	conditions					909:918	different conditions	899:918	different conditions of temperature, pH, and heating time	899:955	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	4	43	theme	Maillard	253:260	arg1	reaction					262:269	Maillard reaction	253:269	Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la	253:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	3	44	with	process	193:199	arg1	adjustment					209:218	pH adjustment	206:218	pH adjustment	206:218	Nanostructures from conjugates of tara gum and α-lactalbumin were obtained via the heat-gelation process with pH adjustment.
31751721	6	45	theme	appropriate	613:623	arg1	2 days					649:654	2 days	649:654	2 days	649:654	The most appropriate time of conjugation was 2 days.
31751721	6	45	theme	appropriate	613:623	arg1	time					625:628	The most appropriate time	604:628	The most appropriate time of conjugation	604:643	The most appropriate time of conjugation was 2 days.
31751721	4	46	theme	free	418:421	arg1	groups					429:434	free amino groups	418:434	free amino groups (% FAG)	418:442	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	46	theme	free	418:421	arg1	FAG					439:441	% FAG	437:441	% FAG	437:441	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	0	47	dep	tara	31:34	arg1	tara					31:34	tara gum and α-lactalbumin	31:56	tara gum and α-lactalbumin	31:56	Nanostructured conjugates from tara gum and α-lactalbumin.
31751721	0	47	dep	tara	31:34	arg1	α-lactalbumin					44:56	α-lactalbumin	44:56	α-lactalbumin	44:56	Nanostructured conjugates from tara gum and α-lactalbumin.
31751721	0	47	dep	tara	31:34	arg1	gum					36:38	gum	36:38	gum	36:38	Nanostructured conjugates from tara gum and α-lactalbumin.
31751721	5	48	theme	intrinsic	553:561	arg1	fluorescence					563:574	intrinsic fluorescence	553:574	intrinsic fluorescence	553:574	Nanostructured systems were characterized by dynamic light scattering, ζ-potential, circular dichroism, and intrinsic fluorescence to evaluate the structures.
31751721	8	49	theme	pH	936:937	arg1	conditions					909:918	different conditions	899:918	different conditions of temperature, pH, and heating time	899:955	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	7	50	theme	spray-dried	661:671	arg1	mixtures					689:696	The spray-dried and lyophilized mixtures	657:696	The spray-dried and lyophilized mixtures	657:696	The spray-dried and lyophilized mixtures presented different values of BI and % FAG (p < 0.05), indicating that the glycosylation was more intense in lyophilized mixtures.
31751721	10	51	from	conjugates	1244:1253	arg1	nanostructures					1224:1237	nanostructures	1224:1237	nanostructures from conjugates of tara gum and α-lactalbumin	1224:1283	The characteristics presented by the studied systems showed that it is possible to obtain nanostructures from conjugates of tara gum and α-lactalbumin.
31751721	4	52	theme	TG	342:343	arg1	mixtures					330:337	lyophilized or spray-dried mixtures	303:337	lyophilized or spray-dried mixtures of TG and α-la	303:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	5	53	theme	light	498:502	arg1	scattering					504:513	dynamic light scattering	490:513	dynamic light scattering	490:513	Nanostructured systems were characterized by dynamic light scattering, ζ-potential, circular dichroism, and intrinsic fluorescence to evaluate the structures.
31751721	4	54	theme	groups	429:434	arg1	BI					396:397	BI	396:397	BI	396:397	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	54	theme	groups	429:434	arg1	percentage					404:413	percentage	404:413	percentage of free amino groups (% FAG)	404:442	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	4	54	theme	groups	429:434	arg1	index					389:393	browning index	380:393	browning index (BI)	380:398	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	9	55	from	decrease	1078:1085	arg1	content					1102:1108	the α-helix content	1090:1108	the α-helix content in spray-dried systems	1090:1131	Analyses of circular dichroism and intrinsic fluorescence showed conformational changes in the nanostructures, mainly a decrease in the α-helix content in spray-dried systems.
31751721	8	56	theme	average	849:855	arg1	sizes					857:861	average sizes	849:861	average sizes lower than 300 nm	849:879	Nanostructures with average sizes lower than 300 nm were formed under different conditions of temperature, pH, and heating time.
31751721	0	57	from	tara	31:34	arg1	conjugates					15:24	Nanostructured conjugates	0:24	Nanostructured conjugates from tara gum and α-lactalbumin.	0:57	Nanostructured conjugates from tara gum and α-lactalbumin.
31751721	4	58	theme	α-la	349:352	arg1	mixtures					330:337	lyophilized or spray-dried mixtures	303:337	lyophilized or spray-dried mixtures of TG and α-la	303:352	The conjugates were produced by Maillard reaction using the dry-heating method in lyophilized or spray-dried mixtures of TG and α-la and were characterized by browning index (BI) and percentage of free amino groups (% FAG).
31751721	6	59	theme	conjugation	633:643	arg1	2 days					649:654	2 days	649:654	2 days	649:654	The most appropriate time of conjugation was 2 days.
31751721	6	59	theme	conjugation	633:643	arg1	time					625:628	The most appropriate time	604:628	The most appropriate time of conjugation	604:643	The most appropriate time of conjugation was 2 days.
31472439	0	0	theme	composite	69:77	arg1	production					79:88	wood plastic composite production	56:88	wood plastic composite production	56:88	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	3	1	theme	SPC	681:683	arg1	bonding					665:671	the interfacial bonding	649:671	the interfacial bonding of both SPC and WPC	649:691	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	4	2	theme	environmental	782:794	arg1	impact					796:801	the environmental impact	778:801	the environmental impact	778:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	0	3	theme	plastic	61:67	arg1	production					79:88	wood plastic composite production	56:88	wood plastic composite production	56:88	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	6	4	theme	bonded	1208:1213	arg1	flour					1220:1224	wood flour	1215:1224	wood flour	1215:1224	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	4	5	from	impact	796:801	arg1	value					767:771	the economic value	754:771	the economic value from the environmental impact	754:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	1	6	theme	novel	154:158	arg1	development					160:170	a novel development	152:170	a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept	152:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	7	theme	circular	233:240	arg1	concept					250:256	the circular economy concept	229:256	the circular economy concept	229:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	2	8	theme	substitution	477:488	arg1	potentials					490:499	the substitution potentials	473:499	the substitution potentials	473:499	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	9	9	theme	WPC	1602:1604	arg1	production					1588:1597	the production	1584:1597	the production of WPC	1584:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	1	10	theme	economy	242:248	arg1	concept					250:256	the circular economy concept	229:256	the circular economy concept	229:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	6	11	theme	microscope	1103:1112	arg1	analysis					1120:1127	Scanning electron microscope (SEM) analysis	1085:1127	Scanning electron microscope (SEM) analysis	1085:1127	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	8	12	dep	better	1422:1427	arg1	economic					1447:1454	economic	1447:1454	economic	1447:1454	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	8	12	dep	better	1422:1427	arg1	environmental					1429:1441	environmental	1429:1441	environmental	1429:1441	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	4	13	theme	economic	758:765	arg1	value					767:771	the economic value	754:771	the economic value from the environmental impact	754:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	2	14	theme	plastic	380:386	arg1	WPC					399:401	wood plastic composite (WPC)	375:402	wood plastic composite (WPC)	375:402	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	5	15	theme	tensile	897:903	arg1	strength					918:925	the tensile and flexural strength	893:925	the tensile and flexural strength of the composites	893:943	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	9	16	from	fibres	1574:1579	arg1	production					1588:1597	the production	1584:1597	the production of WPC	1584:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	2	17	theme	sludge	409:414	arg1	cellulose					416:424	sludge cellulose	409:424	sludge cellulose	409:424	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	3	18	theme	interfacial	653:663	arg1	bonding					665:671	the interfacial bonding	649:671	the interfacial bonding of both SPC and WPC	649:691	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	9	19	theme	promising	1521:1529	arg1	alternative					1543:1553	a promising sustainable alternative	1519:1553	a promising sustainable alternative to wood or natural fibres in the production of WPC	1519:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	6	20	theme	Scanning	1085:1092	arg1	SEM					1115:1117	SEM	1115:1117	SEM	1115:1117	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	6	20	theme	Scanning	1085:1092	arg1	microscope					1103:1112	Scanning electron microscope	1085:1112	Scanning electron microscope (SEM) analysis	1085:1127	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	9	21	from	wood	1558:1561	arg1	production					1588:1597	the production	1584:1597	the production of WPC	1584:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	5	22	theme	bonding	1076:1082	arg1	refinery					1052:1059	the refinery	1048:1059	the refinery of interfacial bonding	1048:1082	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	0	23	theme	circular	2:9	arg1	use					19:21	A circular economy use	0:21	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.	0:130	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	6	24	theme	wood	1215:1218	arg1	flour					1220:1224	wood flour	1215:1224	wood flour	1215:1224	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	9	25	theme	sludge	1499:1504	arg1	cellulose					1506:1514	sludge cellulose	1499:1514	sludge cellulose	1499:1514	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	6	26	theme	sludge	1229:1234	arg1	cellulose					1236:1244	sludge cellulose	1229:1244	sludge cellulose	1229:1244	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	1	27	theme	sludge	175:180	arg1	composite					200:208	sludge cellulose plastic composite	175:208	sludge cellulose plastic composite (SPC)	175:214	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	27	theme	sludge	175:180	arg1	SPC					211:213	SPC	211:213	SPC	211:213	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	3	28	theme	WPC	689:691	arg1	bonding					665:671	the interfacial bonding	649:671	the interfacial bonding of both SPC and WPC	649:691	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	6	29	theme	embedded	1188:1195	arg1	flour					1220:1224	wood flour	1215:1224	wood flour	1215:1224	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	1	30	theme	cellulose	182:190	arg1	composite					200:208	sludge cellulose plastic composite	175:208	sludge cellulose plastic composite (SPC)	175:214	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	30	theme	cellulose	182:190	arg1	SPC					211:213	SPC	211:213	SPC	211:213	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	7	31	theme	comparable	1332:1341	arg1	properties					1352:1361	comparable flexural properties	1332:1361	comparable flexural properties	1332:1361	WPC was marginally more thermally stable than SPC, while SPC suggested comparable flexural properties.
31472439	4	32	theme	circular	723:730	arg1	economy					732:738	circular economy	723:738	circular economy - to decouple the economic value from the environmental impact	723:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	6	33	theme	interface	1158:1166	arg1	improvement					1143:1153	the improvement	1139:1153	the improvement of interface	1139:1166	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	1	34	theme	plastic	192:198	arg1	composite					200:208	sludge cellulose plastic composite	175:208	sludge cellulose plastic composite (SPC)	175:214	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	34	theme	plastic	192:198	arg1	SPC					211:213	SPC	211:213	SPC	211:213	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	0	35	theme	economy	11:17	arg1	use					19:21	A circular economy use	0:21	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.	0:130	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	1	36	theme	recovered	267:275	arg1	cellulose					284:292	recovered sludge cellulose	267:292	recovered sludge cellulose	267:292	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	0	37	theme	eco-efficiency	105:118	arg1	assessment					120:129	eco-efficiency assessment	105:129	eco-efficiency assessment	105:129	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	8	38	theme	assessment	1379:1388	arg1	results					1390:1396	Eco-efficiency assessment results	1364:1396	Eco-efficiency assessment results	1364:1396	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	1	39	theme	composite	200:208	arg1	development					160:170	a novel development	152:170	a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept	152:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	0	40	theme	recovered	26:34	arg1	cellulose					43:51	recovered sludge cellulose	26:51	recovered sludge cellulose	26:51	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	1	41	theme	sludge	277:282	arg1	cellulose					284:292	recovered sludge cellulose	267:292	recovered sludge cellulose	267:292	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	8	42	theme	Eco-efficiency	1364:1377	arg1	results					1390:1396	Eco-efficiency assessment results	1364:1396	Eco-efficiency assessment results	1364:1396	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	5	43	theme	composites	934:943	arg1	strength					918:925	the tensile and flexural strength	893:925	the tensile and flexural strength of the composites	893:943	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	2	44	theme	wood	375:378	arg1	WPC					399:401	wood plastic composite (WPC)	375:402	wood plastic composite (WPC)	375:402	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	9	45	theme	natural	1566:1572	arg1	fibres					1574:1579	natural fibres	1566:1579	natural fibres in the production of WPC	1566:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	4	46	theme	developed	850:858	arg1	process					860:866	the developed process	846:866	the developed process	846:866	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	3	47	theme	maleic	554:559	arg1	MA					572:573	MA	572:573	MA	572:573	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	3	47	theme	maleic	554:559	arg1	anhydride					561:569	maleic anhydride	554:569	maleic anhydride (MA)	554:574	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	0	48	theme	cellulose	43:51	arg1	use					19:21	A circular economy use	0:21	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.	0:130	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	5	49	theme	interfacial	1064:1074	arg1	bonding					1076:1082	interfacial bonding	1064:1082	interfacial bonding	1064:1082	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	9	50	theme	sustainable	1531:1541	arg1	alternative					1543:1553	a promising sustainable alternative	1519:1553	a promising sustainable alternative to wood or natural fibres in the production of WPC	1519:1604	The latter turns sludge cellulose as a promising sustainable alternative to wood or natural fibres in the production of WPC.
31472439	3	51	theme	vinyltrimethoxysilane	580:600	arg1	agents					618:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	8	52	theme	better	1422:1427	arg1	performance					1456:1466	better environmental and economic performance	1422:1466	better environmental and economic performance	1422:1466	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	0	53	theme	sludge	36:41	arg1	cellulose					43:51	recovered sludge cellulose	26:51	recovered sludge cellulose	26:51	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	1	54	theme	wastewater	299:308	arg1	WWTP					327:330	WWTP	327:330	WWTP	327:330	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	54	theme	wastewater	299:308	arg1	plant					320:324	wastewater treatment plant	299:324	wastewater treatment plant (WWTP)	299:331	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	4	55	theme	eco-efficiency	804:817	arg1	analysis					819:826	eco-efficiency analysis	804:826	eco-efficiency analysis	804:826	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	3	56	theme	properties	542:551	arg1	integration					527:537	the integration	523:537	the integration of properties	523:551	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	2	57	from	wood	367:370	arg1	WPC					399:401	wood plastic composite (WPC)	375:402	wood plastic composite (WPC)	375:402	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	1	58	theme	treatment	310:318	arg1	WWTP					327:330	WWTP	327:330	WWTP	327:330	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	1	58	theme	treatment	310:318	arg1	plant					320:324	wastewater treatment plant	299:324	wastewater treatment plant (WWTP)	299:331	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	5	59	theme	flexural	909:916	arg1	strength					918:925	the tensile and flexural strength	893:925	the tensile and flexural strength of the composites	893:943	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	0	60	dep	use	19:21	arg1	Recycling					91:99	Recycling	91:99	Recycling	91:99	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	0	60	dep	use	19:21	arg1	assessment					120:129	eco-efficiency assessment	105:129	eco-efficiency assessment	105:129	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	4	61	theme	economy	732:738	arg1	aim					716:718	the main aim	707:718	the main aim of circular economy - to decouple the economic value from the environmental impact	707:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	8	62	contain	had	1418:1420	arg1	SPC					1414:1416	the SPC	1410:1416	the SPC	1410:1416	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	8	62	contain	had	1418:1420	arg2	performance					1456:1466	better environmental and economic performance	1422:1466	better environmental and economic performance	1422:1466	Eco-efficiency assessment results showed that the SPC had better environmental and economic performance than the WPC.
31472439	7	63	theme	flexural	1343:1350	arg1	properties					1352:1361	comparable flexural properties	1332:1361	comparable flexural properties	1332:1361	WPC was marginally more thermally stable than SPC, while SPC suggested comparable flexural properties.
31472439	4	64	theme	main	711:714	arg1	aim					716:718	the main aim	707:718	the main aim of circular economy - to decouple the economic value from the environmental impact	707:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	1	65	with	line	219:222	arg1	concept					250:256	the circular economy concept	229:256	the circular economy concept	229:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31472439	0	66	theme	wood	56:59	arg1	production					79:88	wood plastic composite production	56:88	wood plastic composite production	56:88	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	3	67	theme	coupling	609:616	arg1	agents					618:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	3	68	theme	anhydride	561:569	arg1	agents					618:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents	554:623	In order to maximise the integration of properties, maleic anhydride (MA) and vinyltrimethoxysilane (VTMS) coupling agents were employed to refine the interfacial bonding of both SPC and WPC.
31472439	2	69	theme	composite	388:396	arg1	WPC					399:401	wood plastic composite (WPC)	375:402	wood plastic composite (WPC)	375:402	Bearing the aim of replacing the wood in wood plastic composite (WPC) with sludge cellulose, WPC was developed in parallel for determining the substitution potentials.
31472439	6	70	theme	electron	1094:1101	arg1	SEM					1115:1117	SEM	1115:1117	SEM	1115:1117	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	6	70	theme	electron	1094:1101	arg1	microscope					1103:1112	Scanning electron microscope	1085:1112	Scanning electron microscope (SEM) analysis	1085:1127	Scanning electron microscope (SEM) analysis confirmed the improvement of interface by identifying well embedded and firmly bonded wood flour or sludge cellulose in the matrix.
31472439	4	71	with	line	697:700	arg1	aim					716:718	the main aim	707:718	the main aim of circular economy - to decouple the economic value from the environmental impact	707:801	In line with the main aim of circular economy - to decouple the economic value from the environmental impact, eco-efficiency analysis was performed for the developed process.
31472439	5	72	from	VTMS	1040:1043	arg1	refinery					1052:1059	the refinery	1048:1059	the refinery of interfacial bonding	1048:1082	The results showed that the tensile and flexural strength of the composites were substantially enhanced after both treatments, while MA appeared to be more efficient than VTMS in the refinery of interfacial bonding.
31472439	0	73	from	use	19:21	arg1	production					79:88	wood plastic composite production	56:88	wood plastic composite production	56:88	A circular economy use of recovered sludge cellulose in wood plastic composite production: Recycling and eco-efficiency assessment.
31472439	1	74	from	development	160:170	arg1	line					219:222	line	219:222	line with the circular economy concept	219:256	This paper presents a novel development of sludge cellulose plastic composite (SPC) in line with the circular economy concept by using recovered sludge cellulose from wastewater treatment plant (WWTP).
31228072	0	0	theme	cationic	105:112	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	1	1	theme	etherifying	318:328	arg1	agent					330:334	the etherifying agent	314:334	the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively	314:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	1	theme	etherifying	318:328	arg1	starch					181:186	a crosslinked carboxymethyl starch	153:186	a crosslinked carboxymethyl starch (CCMS)	153:193	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	2	theme	anatase	825:831	arg1	TiO2					833:836	anatase TiO2	825:836	anatase TiO2	825:836	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	1	3	theme	crosslinked	155:165	arg1	agent					330:334	the etherifying agent	314:334	the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively	314:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	3	theme	crosslinked	155:165	arg1	CCMS					189:192	CCMS	189:192	CCMS	189:192	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	3	theme	crosslinked	155:165	arg1	starch					181:186	a crosslinked carboxymethyl starch	153:186	a crosslinked carboxymethyl starch (CCMS)	153:193	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	7	4	theme	significant	1170:1180	arg1	synergies					1210:1218	significant adsorption-photodegradation synergies	1170:1218	significant adsorption-photodegradation synergies	1170:1218	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	7	5	theme	TiO2/CCMS	1083:1091	arg1	composite					1093:1101	the TiO2/CCMS composite	1079:1101	the TiO2/CCMS composite	1079:1101	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	2	6	theme	CCMS	473:476	arg1	surface					458:464	the surface	454:464	the surface of the CCMS	454:476	Nano-TiO2 was loaded onto the surface of the CCMS by the sol-gel method to obtain a TiO2/CCMS composite.
31228072	1	7	theme	carboxymethyl	167:179	arg1	agent					330:334	the etherifying agent	314:334	the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively	314:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	7	theme	carboxymethyl	167:179	arg1	CCMS					189:192	CCMS	189:192	CCMS	189:192	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	7	theme	carboxymethyl	167:179	arg1	starch					181:186	a crosslinked carboxymethyl starch	153:186	a crosslinked carboxymethyl starch (CCMS)	153:193	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	2	8	theme	sol-gel	485:491	arg1	method					493:498	the sol-gel method	481:498	the sol-gel method to obtain a TiO2/CCMS composite	481:530	Nano-TiO2 was loaded onto the surface of the CCMS by the sol-gel method to obtain a TiO2/CCMS composite.
31228072	0	9	theme	yellow	121:126	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	5	10	theme	CCMS	943:946	arg1	surface					928:934	the surface	924:934	the surface of the CCMS	924:946	The surface morphology obtained by SEM showed that there were nano-TiO2 particles on the surface of the CCMS.
31228072	5	11	theme	surface	843:849	arg1	morphology					851:860	The surface morphology	839:860	The surface morphology obtained by SEM	839:876	The surface morphology obtained by SEM showed that there were nano-TiO2 particles on the surface of the CCMS.
31228072	4	12	theme	XRD	707:709	arg1	patterns					723:730	XRD diffraction patterns	707:730	XRD diffraction patterns	707:730	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	0	13	theme	golden	114:119	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	4	14	theme	TiO2	833:836	arg1	combination					797:807	a combination	795:807	a combination of the CCMS and anatase TiO2	795:836	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	14	theme	TiO2	833:836	arg1	form					759:762	the crystal form	747:762	the crystal form of the TiO2/CCMS composite	747:789	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	0	15	link	TiO2/crosslinked	2:17	arg1	composite					40:48	A TiO2/crosslinked carboxymethyl starch composite	0:48	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption	0:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	15	link	TiO2/crosslinked	2:17	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	16	theme	dye	133:135	arg1	photodegradation					85:100	photodegradation	85:100	photodegradation of cationic golden yellow X-GL dye	85:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	16	theme	dye	133:135	arg1	composite					40:48	A TiO2/crosslinked carboxymethyl starch composite	0:48	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption	0:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	16	theme	dye	133:135	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	4	17	contain	contained	687:695	arg2	titanium					697:704	titanium	697:704	titanium	697:704	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	17	contain	contained	687:695	arg1	composition					639:649	the surface chemical composition	618:649	the surface chemical composition of the TiO2/CCMS composite material	618:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	7	18	theme	UV	1126:1127	arg1	irradiation					1129:1139	UV irradiation	1126:1139	UV irradiation	1126:1139	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	0	19	theme	X-GL	128:131	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	7	20	theme	adsorption-photodegradation	1036:1062	arg1	performance					1064:1074	The adsorption-photodegradation performance	1032:1074	The adsorption-photodegradation performance of the TiO2/CCMS composite	1032:1101	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	0	21	theme	TiO2/crosslinked	2:17	arg1	composite					40:48	A TiO2/crosslinked carboxymethyl starch composite	0:48	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption	0:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	21	theme	TiO2/crosslinked	2:17	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	1	22	theme	crosslinking	356:367	arg1	series					346:351	a series	344:351	a series of crosslinking, alkalization, and etherification reactions, respectively	344:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	23	theme	CCMS	816:819	arg1	combination					797:807	a combination	795:807	a combination of the CCMS and anatase TiO2	795:836	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	23	theme	CCMS	816:819	arg1	form					759:762	the crystal form	747:762	the crystal form of the TiO2/CCMS composite	747:789	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	24	theme	surface	622:628	arg1	composition					639:649	the surface chemical composition	618:649	the surface chemical composition of the TiO2/CCMS composite material	618:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	0	25	theme	starch	33:38	arg1	composite					40:48	A TiO2/crosslinked carboxymethyl starch composite	0:48	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption	0:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	25	theme	starch	33:38	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	1	26	theme	crosslinking	269:280	arg1	agent					282:286	the crosslinking agent	265:286	the crosslinking agent	265:286	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	27	link	crosslinked	155:165	arg1	agent					330:334	the etherifying agent	314:334	the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively	314:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	27	link	crosslinked	155:165	arg1	CCMS					189:192	CCMS	189:192	CCMS	189:192	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	1	27	link	crosslinked	155:165	arg1	starch					181:186	a crosslinked carboxymethyl starch	153:186	a crosslinked carboxymethyl starch (CCMS)	153:193	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	28	theme	diffraction	711:721	arg1	patterns					723:730	XRD diffraction patterns	707:730	XRD diffraction patterns	707:730	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	0	29	theme	carboxymethyl	19:31	arg1	composite					40:48	A TiO2/crosslinked carboxymethyl starch composite	0:48	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption	0:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	0	29	theme	carboxymethyl	19:31	arg1	dye					133:135	cationic golden yellow X-GL dye	105:135	cationic golden yellow X-GL dye	105:135	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	3	30	theme	TiO2/CCMS	537:545	arg1	composite					547:555	The TiO2/CCMS composite	533:555	The TiO2/CCMS composite	533:555	The TiO2/CCMS composite was characterized by XPS, XRD, SEM, and BET.
31228072	6	31	theme	composite	992:1000	arg1	area					970:973	The specific surface area	949:973	The specific surface area of the TiO2/CCMS composite	949:1000	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	6	31	theme	composite	992:1000	arg1	larger					1006:1011	larger	1006:1011	larger	1006:1011	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	2	32	theme	TiO2/CCMS	512:520	arg1	composite					522:530	a TiO2/CCMS composite	510:530	a TiO2/CCMS composite	510:530	Nano-TiO2 was loaded onto the surface of the CCMS by the sol-gel method to obtain a TiO2/CCMS composite.
31228072	1	33	theme	alkalization	370:381	arg1	series					346:351	a series	344:351	a series of crosslinking, alkalization, and etherification reactions, respectively	344:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	34	theme	composite	781:789	arg1	combination					797:807	a combination	795:807	a combination of the CCMS and anatase TiO2	795:836	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	34	theme	composite	781:789	arg1	form					759:762	the crystal form	747:762	the crystal form of the TiO2/CCMS composite	747:789	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	6	35	theme	TiO2/CCMS	982:990	arg1	composite					992:1000	the TiO2/CCMS composite	978:1000	the TiO2/CCMS composite	978:1000	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	4	36	theme	TiO2/CCMS	771:779	arg1	composite					781:789	the TiO2/CCMS composite	767:789	the TiO2/CCMS composite	767:789	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	1	37	theme	corn	213:216	arg1	starch					218:223	corn starch	213:223	corn starch as the raw material	213:243	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	7	38	theme	composite	1093:1101	arg1	performance					1064:1074	The adsorption-photodegradation performance	1032:1074	The adsorption-photodegradation performance of the TiO2/CCMS composite	1032:1101	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	1	39	theme	chloroacetic	293:304	arg1	acid					306:309	chloroacetic acid	293:309	chloroacetic acid	293:309	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	40	theme	composite	668:676	arg1	material					678:685	the TiO2/CCMS composite material	654:685	the TiO2/CCMS composite material	654:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	0	41	theme	high-efficiency	54:68	arg1	adsorption					70:79	high-efficiency adsorption	54:79	high-efficiency adsorption	54:79	A TiO2/crosslinked carboxymethyl starch composite for high-efficiency adsorption and photodegradation of cationic golden yellow X-GL dye.
31228072	1	42	theme	etherification	388:401	arg1	reactions					403:411	etherification reactions	388:411	etherification reactions	388:411	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	4	43	dep	CCMS	816:819	arg1	the					812:814	the	812:814	the	812:814	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	44	theme	TiO2/CCMS	658:666	arg1	material					678:685	the TiO2/CCMS composite material	654:685	the TiO2/CCMS composite material	654:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	1	45	theme	reactions	403:411	arg1	series					346:351	a series	344:351	a series of crosslinking, alkalization, and etherification reactions, respectively	344:425	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
31228072	5	46	theme	nano-TiO2	901:909	arg1	particles					911:919	nano-TiO2 particles	901:919	nano-TiO2 particles	901:919	The surface morphology obtained by SEM showed that there were nano-TiO2 particles on the surface of the CCMS.
31228072	4	47	theme	crystal	751:757	arg1	combination					797:807	a combination	795:807	a combination of the CCMS and anatase TiO2	795:836	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	47	theme	crystal	751:757	arg1	form					759:762	the crystal form	747:762	the crystal form of the TiO2/CCMS composite	747:789	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	4	48	theme	material	678:685	arg1	composition					639:649	the surface chemical composition	618:649	the surface chemical composition of the TiO2/CCMS composite material	618:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	7	49	theme	adsorption-photodegradation	1182:1208	arg1	synergies					1210:1218	significant adsorption-photodegradation synergies	1170:1218	significant adsorption-photodegradation synergies	1170:1218	The adsorption-photodegradation performance of the TiO2/CCMS composite was also studied under UV irradiation, and the results showed that significant adsorption-photodegradation synergies occurred.
31228072	6	50	theme	surface	962:968	arg1	area					970:973	The specific surface area	949:973	The specific surface area of the TiO2/CCMS composite	949:1000	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	6	50	theme	surface	962:968	arg1	larger					1006:1011	larger	1006:1011	larger	1006:1011	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	6	51	theme	specific	953:960	arg1	area					970:973	The specific surface area	949:973	The specific surface area of the TiO2/CCMS composite	949:1000	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	6	51	theme	specific	953:960	arg1	larger					1006:1011	larger	1006:1011	larger	1006:1011	The specific surface area of the TiO2/CCMS composite was larger than that of CCMS.
31228072	4	52	theme	chemical	630:637	arg1	composition					639:649	the surface chemical composition	618:649	the surface chemical composition of the TiO2/CCMS composite material	618:685	XPS showed that the surface chemical composition of the TiO2/CCMS composite material contained titanium; XRD diffraction patterns indicated that the crystal form of the TiO2/CCMS composite was a combination of the CCMS and anatase TiO2.
31228072	1	53	theme	raw	232:234	arg1	material					236:243	the raw material	228:243	the raw material	228:243	In this paper, a crosslinked carboxymethyl starch (CCMS) was prepared with corn starch as the raw material, epichlorohydrin as the crosslinking agent, and chloroacetic acid as the etherifying agent through a series of crosslinking, alkalization, and etherification reactions, respectively.
30196422	6	0	theme	@	1059:1059	arg1	NCs					1066:1068	CNPs@Fe3O4 NCs	1055:1068	CNPs@Fe3O4 NCs	1055:1068	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	9	1	theme	On-plate	1643:1650	arg1	desalting					1652:1660	On-plate desalting	1643:1660	On-plate desalting	1643:1660	On-plate desalting enhanced sensitivity by reducing sample loss.
30196422	8	2	theme	biological	1623:1632	arg1	samples					1634:1640	biological samples	1623:1640	biological samples	1623:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	6	3	theme	several	1134:1140	arg1	orders					1142:1147	several orders	1134:1147	several orders of magnitude	1134:1160	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	1	4	theme	biological	163:172	arg1	folding					200:206	protein folding	192:206	protein folding	192:206	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	4	theme	biological	163:172	arg1	transduction					219:230	signal transduction	212:230	signal transduction	212:230	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	4	theme	biological	163:172	arg1	processes					174:182	many biological processes	158:182	many biological processes such as protein folding and signal transduction	158:230	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	9	5	theme	sample	1695:1700	arg1	loss					1702:1705	sample loss	1695:1705	sample loss	1695:1705	On-plate desalting enhanced sensitivity by reducing sample loss.
30196422	6	6	theme	glycan	1107:1112	arg1	intensity					1121:1129	glycan signal intensity	1107:1129	glycan signal intensity	1107:1129	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	4	7	theme	times	651:655	arg1	ease					614:617	ease	614:617	ease of operation and short analysis times	614:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	2	8	theme	different	280:288	arg1	diseases					300:307	different mammalian diseases	280:307	different mammalian diseases such as breast and liver cancers	280:340	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	2	8	theme	different	280:288	arg1	breast					317:322	breast	317:322	breast	317:322	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	2	8	theme	different	280:288	arg1	cancers					334:340	liver cancers	328:340	liver cancers	328:340	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	8	9	theme	CNPs	1418:1421	arg1	NCs					1429:1431	CNPs@Fe3O4 NCs	1418:1431	CNPs@Fe3O4 NCs	1418:1431	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	5	10	theme	magnetic	844:851	arg1	matrix					917:922	a new MALDI matrix	905:922	a new MALDI matrix	905:922	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	10	theme	magnetic	844:851	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	10	theme	magnetic	844:851	arg1	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	10	theme	magnetic	844:851	arg1	co-matrix					927:935	co-matrix	927:935	co-matrix	927:935	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	8	11	theme	Fe3O4	1423:1427	arg1	NCs					1429:1431	CNPs@Fe3O4 NCs	1418:1431	CNPs@Fe3O4 NCs	1418:1431	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	6	12	theme	in-source	1209:1217	arg1	ISD					1226:1228	ISD	1226:1228	ISD	1226:1228	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	6	12	theme	in-source	1209:1217	arg1	decay					1219:1223	in-source decay	1209:1223	in-source decay (ISD) fragmentation	1209:1243	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	1	13	gly	glycosylated	122:133	arg1	proteins					109:116	all known proteins	99:116	all known proteins	99:116	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	13	gly	glycosylated	122:133	arg1	%					94:94	More than 50%	82:94	More than 50% of all known proteins	82:116	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	5	14	theme	human	1012:1016	arg1	samples					1030:1036	human blood serum samples	1012:1036	human blood serum samples	1012:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	1	15	theme	known	103:107	arg1	proteins					109:116	all known proteins	99:116	all known proteins	99:116	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	7	16	theme	simultaneous	1330:1341	arg1	study					1343:1347	the simultaneous study	1326:1347	the simultaneous study of intact and fragmented glycans	1326:1380	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	4	17	theme	ionization	724:733	arg1	efficiency					735:744	their low ionization efficiency	714:744	their low ionization efficiency	714:744	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	5	18	theme	serum	1024:1028	arg1	samples					1030:1036	human blood serum samples	1012:1036	human blood serum samples	1012:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	0	19	theme	MS-based	62:69	arg1	glycomics					71:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.
30196422	5	20	theme	Fe3O4	881:885	arg1	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	20	theme	Fe3O4	881:885	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	8	21	theme	MALDI-MS	1527:1534	arg1	analysis					1536:1543	direct MALDI-MS analysis	1520:1543	direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples	1520:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	4	22	theme	structures	699:708	arg1	complexity					678:687	the complexity	674:687	the complexity of glycan structures and their low ionization efficiency	674:744	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	23	theme	matrix-assisted	507:521	arg1	spectrometry					556:567	matrix-assisted laser desorption ionization-mass spectrometry	507:567	matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS)	507:578	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	23	theme	matrix-assisted	507:521	arg1	MALDI-MS					570:577	MALDI-MS	570:577	MALDI-MS	570:577	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	5	24	attach	derived	965:971	arg1	samples					1030:1036	human blood serum samples	1012:1036	human blood serum samples	1012:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	24	attach	derived	965:971	arg1	glycoproteins					994:1006	different model glycoproteins	978:1006	different model glycoproteins	978:1006	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	24	attach	derived	965:971	arg2	glycans					957:963	glycans	957:963	glycans derived from different model glycoproteins and human blood serum samples	957:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	3	25	theme	glycans	374:380	arg1	characterization					354:369	characterization	354:369	characterization of glycans	354:380	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	0	26	theme	Magnetic	0:7	arg1	carbon					9:14	Magnetic carbon	0:14	Magnetic carbon	0:14	Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.
30196422	5	27	theme	MALDI	911:915	arg1	matrix					917:922	a new MALDI matrix	905:922	a new MALDI matrix	905:922	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	27	theme	MALDI	911:915	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	7	28	theme	NCs	1295:1297	arg1	concentrations					1299:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	7	29	theme	@	1288:1288	arg1	concentrations					1299:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	8	30	theme	direct	1520:1525	arg1	analysis					1536:1543	direct MALDI-MS analysis	1520:1543	direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples	1520:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	4	31	theme	sensitive	811:819	arg1	analysis					828:835	sensitive glycan analysis	811:835	sensitive glycan analysis	811:835	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	5	32	theme	model	988:992	arg1	glycoproteins					994:1006	different model glycoproteins	978:1006	different model glycoproteins	978:1006	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	4	33	theme	desorption	529:538	arg1	spectrometry					556:567	matrix-assisted laser desorption ionization-mass spectrometry	507:567	matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS)	507:578	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	33	theme	desorption	529:538	arg1	MALDI-MS					570:577	MALDI-MS	570:577	MALDI-MS	570:577	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	2	34	theme	liver	328:332	arg1	cancers					334:340	liver cancers	328:340	liver cancers	328:340	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	0	35	theme	co-matrix	42:50	arg1	glycomics					71:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.
30196422	8	36	theme	unpurified	1548:1557	arg1	glycans					1573:1579	unpurified permethylated glycans	1548:1579	unpurified permethylated glycans derived from both model glycoproteins and biological samples	1548:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	5	37	theme	glycans	957:963	arg1	analysis					945:952	the analysis	941:952	the analysis of glycans derived from different model glycoproteins and human blood serum samples	941:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	8	38	theme	glycans	1573:1579	arg1	analysis					1536:1543	direct MALDI-MS analysis	1520:1543	direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples	1520:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	1	39	theme	signal	212:217	arg1	transduction					219:230	signal transduction	212:230	signal transduction	212:230	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	6	40	theme	Fe3O4	1060:1064	arg1	NCs					1066:1068	CNPs@Fe3O4 NCs	1055:1068	CNPs@Fe3O4 NCs	1055:1068	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	6	41	theme	CNPs	1055:1058	arg1	NCs					1066:1068	CNPs@Fe3O4 NCs	1055:1068	CNPs@Fe3O4 NCs	1055:1068	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	8	42	theme	model	1599:1603	arg1	glycoproteins					1605:1617	model glycoproteins	1599:1617	model glycoproteins	1599:1617	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	3	43	theme	human	482:486	arg1	diseases					488:495	many human diseases	477:495	many human diseases	477:495	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	5	44	theme	carbon	853:858	arg1	matrix					917:922	a new MALDI matrix	905:922	a new MALDI matrix	905:922	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	44	theme	carbon	853:858	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	44	theme	carbon	853:858	arg1	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	44	theme	carbon	853:858	arg1	co-matrix					927:935	co-matrix	927:935	co-matrix	927:935	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	1	45	theme	many	158:161	arg1	folding					200:206	protein folding	192:206	protein folding	192:206	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	45	theme	many	158:161	arg1	transduction					219:230	signal transduction	212:230	signal transduction	212:230	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	45	theme	many	158:161	arg1	processes					174:182	many biological processes	158:182	many biological processes such as protein folding and signal transduction	158:230	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	3	46	dep	development	446:456	arg1	the					442:444	the	442:444	the	442:444	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	6	47	theme	signal	1114:1119	arg1	intensity					1121:1129	glycan signal intensity	1107:1129	glycan signal intensity	1107:1129	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	2	48	theme	mammalian	290:298	arg1	diseases					300:307	different mammalian diseases	280:307	different mammalian diseases such as breast and liver cancers	280:340	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	2	48	theme	mammalian	290:298	arg1	breast					317:322	breast	317:322	breast	317:322	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	2	48	theme	mammalian	290:298	arg1	cancers					334:340	liver cancers	328:340	liver cancers	328:340	Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers.
30196422	7	49	theme	pseudo-MS3	1387:1396	arg1	analysis					1398:1405	pseudo-MS3 analysis	1387:1405	pseudo-MS3 analysis	1387:1405	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	8	50	theme	@	1422:1422	arg1	NCs					1429:1431	CNPs@Fe3O4 NCs	1418:1431	CNPs@Fe3O4 NCs	1418:1431	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	4	51	theme	analysis	642:649	arg1	times					651:655	short analysis times	636:655	short analysis times	636:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	5	52	theme	CNPs	876:879	arg1	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	52	theme	CNPs	876:879	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	7	53	theme	fragmented	1363:1372	arg1	glycans					1374:1380	intact and fragmented glycans	1352:1380	intact and fragmented glycans	1352:1380	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	7	54	theme	intact	1352:1357	arg1	glycans					1374:1380	intact and fragmented glycans	1352:1380	intact and fragmented glycans	1352:1380	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	5	55	theme	blood	1018:1022	arg1	samples					1030:1036	human blood serum samples	1012:1036	human blood serum samples	1012:1036	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	1	56	theme	proteins	109:116	arg1	proteins					109:116	all known proteins	99:116	all known proteins	99:116	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	1	56	theme	proteins	109:116	arg1	%					94:94	More than 50%	82:94	More than 50% of all known proteins	82:116	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	7	57	theme	glycans	1374:1380	arg1	study					1343:1347	the simultaneous study	1326:1347	the simultaneous study of intact and fragmented glycans	1326:1380	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	7	57	theme	glycans	1374:1380	arg1	analysis					1398:1405	pseudo-MS3 analysis	1387:1405	pseudo-MS3 analysis	1387:1405	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	4	58	theme	low	720:722	arg1	efficiency					735:744	their low ionization efficiency	714:744	their low ionization efficiency	714:744	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	5	59	used	used	897:900	arg2	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	59	used	used	897:900	arg2	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	59	used	used	897:900	arg2	co-matrix					927:935	co-matrix	927:935	co-matrix	927:935	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	59	used	used	897:900	arg2	matrix					917:922	a new MALDI matrix	905:922	a new MALDI matrix	905:922	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	60	theme	@	880:880	arg1	NCs					887:889	CNPs@Fe3O4 NCs	876:889	CNPs@Fe3O4 NCs	876:889	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	60	theme	@	880:880	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	8	61	theme	desalt	1466:1471	arg1	samples					1473:1479	desalt samples	1466:1479	desalt samples	1466:1479	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	6	62	theme	magnitude	1152:1160	arg1	orders					1142:1147	several orders	1134:1147	several orders of magnitude	1134:1160	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	10	63	theme	Graphical	1708:1716	arg1	abstract					1718:1725	Graphical abstract	1708:1725	Graphical abstract	1708:1725	Graphical abstract ᅟ.
30196422	4	64	theme	laser	523:527	arg1	spectrometry					556:567	matrix-assisted laser desorption ionization-mass spectrometry	507:567	matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS)	507:578	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	64	theme	laser	523:527	arg1	MALDI-MS					570:577	MALDI-MS	570:577	MALDI-MS	570:577	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	65	theme	glycan	692:697	arg1	structures					699:708	glycan structures	692:708	glycan structures	692:708	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	8	66	gly	glycoproteins	1605:1617	arg1	glycoproteins					1605:1617	model glycoproteins	1599:1617	model glycoproteins	1599:1617	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	8	67	attach	derived	1581:1587	arg2	glycans					1573:1579	unpurified permethylated glycans	1548:1579	unpurified permethylated glycans derived from both model glycoproteins and biological samples	1548:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	8	67	attach	derived	1581:1587	arg1	samples					1634:1640	biological samples	1623:1640	biological samples	1623:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	8	67	attach	derived	1581:1587	arg1	glycoproteins					1605:1617	model glycoproteins	1599:1617	model glycoproteins	1599:1617	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	8	68	theme	MALDI	1493:1497	arg1	plate					1499:1503	MALDI plate	1493:1503	MALDI plate	1493:1503	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	5	69	theme	new	907:909	arg1	matrix					917:922	a new MALDI matrix	905:922	a new MALDI matrix	905:922	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	5	69	theme	new	907:909	arg1	nanocomposites					860:873	magnetic carbon nanocomposites	844:873	magnetic carbon nanocomposites (CNPs@Fe3O4 NCs)	844:890	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	7	70	theme	Fe3O4	1289:1293	arg1	concentrations					1299:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	4	71	theme	efficiency	735:744	arg1	complexity					678:687	the complexity	674:687	the complexity of glycan structures and their low ionization efficiency	674:744	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	3	72	theme	better	418:423	arg1	understanding					425:437	a better understanding	416:437	a better understanding of the development and progression of many human diseases	416:495	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	7	73	theme	CNPs	1284:1287	arg1	concentrations					1299:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	CNPs@Fe3O4 NCs concentrations	1284:1312	The latter was attained by modulating CNPs@Fe3O4 NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS3 analysis.
30196422	5	74	gly	glycoproteins	994:1006	arg1	glycoproteins					994:1006	different model glycoproteins	978:1006	different model glycoproteins	978:1006	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	4	75	theme	several	587:593	arg1	advantages					595:604	several advantages	587:604	several advantages such as ease of operation and short analysis times	587:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	75	theme	several	587:593	arg1	ease					614:617	ease	614:617	ease of operation and short analysis times	614:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	3	76	theme	progression	462:472	arg1	understanding					425:437	a better understanding	416:437	a better understanding of the development and progression of many human diseases	416:495	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	0	77	theme	MALDI	36:40	arg1	glycomics					71:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.
30196422	5	78	theme	different	978:986	arg1	glycoproteins					994:1006	different model glycoproteins	978:1006	different model glycoproteins	978:1006	Here, magnetic carbon nanocomposites (CNPs@Fe3O4 NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples.
30196422	4	79	theme	ionization-mass	540:554	arg1	spectrometry					556:567	matrix-assisted laser desorption ionization-mass spectrometry	507:567	matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS)	507:578	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	4	79	theme	ionization-mass	540:554	arg1	MALDI-MS					570:577	MALDI-MS	570:577	MALDI-MS	570:577	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	3	80	theme	development	446:456	arg1	understanding					425:437	a better understanding	416:437	a better understanding of the development and progression of many human diseases	416:495	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	0	81	theme	enhancing	52:60	arg1	glycomics					71:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	a MALDI co-matrix enhancing MS-based glycomics	34:79	Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics.
30196422	8	82	theme	permethylated	1559:1571	arg1	glycans					1573:1579	unpurified permethylated glycans	1548:1579	unpurified permethylated glycans derived from both model glycoproteins and biological samples	1548:1640	Moreover, CNPs@Fe3O4 NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples.
30196422	4	83	theme	glycan	821:826	arg1	analysis					828:835	sensitive glycan analysis	811:835	sensitive glycan analysis	811:835	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	3	84	theme	diseases	488:495	arg1	development					446:456	development	446:456	development	446:456	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	3	84	theme	diseases	488:495	arg1	progression					462:472	progression	462:472	progression	462:472	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	6	85	theme	decay	1219:1223	arg1	fragmentation					1231:1243	in-source decay (ISD) fragmentation	1209:1243	in-source decay (ISD) fragmentation	1209:1243	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	4	86	theme	operation	622:630	arg1	ease					614:617	ease	614:617	ease of operation and short analysis times	614:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
30196422	3	87	theme	many	477:480	arg1	diseases					488:495	many human diseases	477:495	many human diseases	477:495	Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases.
30196422	1	88	theme	protein	192:198	arg1	folding					200:206	protein folding	192:206	protein folding	192:206	More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction.
30196422	6	89	theme	NCs	1066:1068	arg1	addition					1043:1050	The addition	1039:1050	The addition of CNPs@Fe3O4 NCs to the matrix	1039:1082	The addition of CNPs@Fe3O4 NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation.
30196422	4	90	theme	short	636:640	arg1	times					651:655	short analysis times	636:655	short analysis times	636:655	Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis.
31355634	6	0	theme	mechanical	1387:1396	arg1	tuning					1377:1382	tuning	1377:1382	tuning of mechanical, thermal, and surface properties of the nanopapers produced	1377:1456	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	2	1	theme	foam-like	383:391	arg1	properties					404:413	foam-like mechanical properties	383:413	foam-like mechanical properties	383:413	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	5	2	theme	organic	1262:1268	arg1	residues					1276:1283	organic lipid residues	1262:1283	organic lipid residues in the nanopapers	1262:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	3	theme	agricultural	253:264	arg1	wastes					281:286	agricultural and industrial wastes	253:286	agricultural and industrial wastes	253:286	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	3	4	theme	fungal	707:712	arg1	microfilaments					714:727	fungal microfilaments	707:727	fungal microfilaments	707:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	6	5	dep	HCl	1304:1306	arg1	treatments					1316:1325	treatments	1316:1325	treatments	1316:1325	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	1	6	theme	commercial	170:179	arg1	interest					194:201	commercial and academic interest	170:201	interest	194:201	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	5	7	theme	lipid	1270:1274	arg1	residues					1276:1283	organic lipid residues	1262:1283	organic lipid residues in the nanopapers	1262:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	8	theme	hydrophobic	1200:1210	arg1	properties					1220:1229	useful hydrophobic surface properties	1193:1229	useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers	1193:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	9	dep	low-cost	293:300	arg1	sustainable					303:313	sustainable	303:313	sustainable	303:313	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	4	10	theme	common	803:808	arg1	reference					819:827	a common mushroom reference	801:827	a common mushroom reference	801:827	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	5	11	theme	residues	1276:1283	arg1	presence					1250:1257	the presence	1246:1257	the presence of organic lipid residues in the nanopapers	1246:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	12	theme	industrial	270:279	arg1	wastes					281:286	agricultural and industrial wastes	253:286	agricultural and industrial wastes	253:286	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	3	13	from	wastes	670:675	arg1	form					699:702	the form	695:702	the form of fungal microfilaments	695:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	5	14	from	residues	1276:1283	arg1	nanopapers					1292:1301	the nanopapers	1288:1301	the nanopapers	1288:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	4	15	theme	byproduct	887:895	arg1	molasses					897:904	sugarcane byproduct molasses	877:904	sugarcane byproduct molasses	877:904	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	3	16	theme	natural	576:582	arg1	chitin					605:610	chitin	605:610	chitin	605:610	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	16	theme	natural	576:582	arg1	nanofibrils					584:594	natural nanofibrils	576:594	natural nanofibrils	576:594	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	16	theme	natural	576:582	arg1	chitosan					616:623	chitosan	616:623	chitosan	616:623	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	17	theme	Fungal	483:488	arg1	growth					490:495	Fungal growth	483:495	Fungal growth	483:495	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	17	theme	Fungal	483:488	arg1	method					541:546	a low-cost method	530:546	a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments	530:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	18	from	byproducts	681:690	arg1	form					699:702	the form	695:702	the form of fungal microfilaments	695:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	5	19	theme	MPa	1139:1141	arg1	values					1119:1124	values	1119:1124	values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom)	1119:1175	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	4	20	theme	sugarcane	877:885	arg1	molasses					897:904	sugarcane byproduct molasses	877:904	sugarcane byproduct molasses	877:904	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	3	21	theme	nanofibrils	584:594	arg1	generation					562:571	on-demand generation	552:571	on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments	552:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	1	22	theme	academic	185:192	arg1	interest					194:201	commercial and academic interest	170:201	interest	194:201	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	0	23	theme	Low-Cost	14:21	arg1	Mycelium					23:30	Waste-Derived Low-Cost Mycelium	0:30	Waste-Derived Low-Cost Mycelium	0:30	Waste-Derived Low-Cost Mycelium Nanopapers with Tunable Mechanical and Surface Properties.
31355634	5	24	from	nanopapers	1292:1301	arg1	presence					1250:1257	the presence	1246:1257	the presence of organic lipid residues in the nanopapers	1246:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	25	theme	crustacean-derived	981:998	arg1	chitin					1000:1005	crustacean-derived chitin	981:1005	crustacean-derived chitin	981:1005	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	0	26	theme	Waste-Derived	0:12	arg1	Mycelium					23:30	Waste-Derived Low-Cost Mycelium	0:30	Waste-Derived Low-Cost Mycelium	0:30	Waste-Derived Low-Cost Mycelium Nanopapers with Tunable Mechanical and Surface Properties.
31355634	5	27	theme	useful	1193:1198	arg1	properties					1220:1229	useful hydrophobic surface properties	1193:1229	useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers	1193:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	3	28	used	utilized	518:525	arg2	method					541:546	a low-cost method	530:546	a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments	530:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	28	used	utilized	518:525	arg2	growth					490:495	Fungal growth	483:495	Fungal growth	483:495	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	6	29	dep	mechanical	1387:1396	arg1	thermal					1399:1405	thermal	1399:1405	thermal	1399:1405	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	1	30	theme	low-cost	293:300	arg1	materials					325:333	low-cost, sustainable composite materials	293:333	low-cost, sustainable composite materials	293:333	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	1	31	theme	vegetative	105:114	arg1	Mycelium					91:98	Mycelium	91:98	Mycelium	91:98	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	1	31	theme	vegetative	105:114	arg1	growth					116:121	the vegetative growth	101:121	the vegetative growth of filamentous fungi	101:142	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	5	32	theme	tensile	1058:1064	arg1	strengths					1066:1074	much higher tensile strengths	1046:1074	much higher tensile strengths	1046:1074	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	2	33	theme	filler	462:467	arg1	constituents					469:480	their weak organic filler constituents	443:480	their weak organic filler constituents	443:480	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	3	34	theme	on-demand	552:560	arg1	generation					562:571	on-demand generation	552:571	on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments	552:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	2	35	theme	organic	454:460	arg1	constituents					469:480	their weak organic filler constituents	443:480	their weak organic filler constituents	443:480	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	6	36	theme	properties	1420:1429	arg1	tuning					1377:1382	tuning	1377:1382	tuning of mechanical, thermal, and surface properties of the nanopapers produced	1377:1456	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	4	37	theme	mycelium	859:866	arg1	species					841:847	various species	833:847	various species of fungal mycelium grown on sugarcane byproduct molasses	833:904	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	4	37	theme	mycelium	859:866	arg1	reference					819:827	a common mushroom reference	801:827	a common mushroom reference	801:827	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	7	38	theme	applications	1513:1524	arg1	coatings					1536:1543	coatings	1536:1543	coatings	1536:1543	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	38	theme	applications	1513:1524	arg1	membranes					1546:1554	membranes	1546:1554	membranes	1546:1554	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	38	theme	applications	1513:1524	arg1	range					1504:1508	a wide range	1497:1508	a wide range of applications including coatings, membranes, packaging, and paper	1497:1576	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	38	theme	applications	1513:1524	arg1	paper					1572:1576	paper	1572:1576	paper	1572:1576	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	38	theme	applications	1513:1524	arg1	packaging					1557:1565	packaging	1557:1565	packaging	1557:1565	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	2	39	theme	weak	449:452	arg1	constituents					469:480	their weak organic filler constituents	443:480	their weak organic filler constituents	443:480	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	5	40	theme	Polymer	907:913	arg1	yields					915:920	Polymer yields	907:920	Polymer yields of ∼10-26%	907:931	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	41	theme	composite	315:323	arg1	materials					325:333	low-cost, sustainable composite materials	293:333	low-cost, sustainable composite materials	293:333	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	6	42	theme	surface	1412:1418	arg1	properties					1420:1429	mechanical, thermal, and surface properties	1387:1429	properties	1420:1429	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	3	43	theme	liquid	663:668	arg1	wastes					670:675	liquid wastes	663:675	liquid wastes	663:675	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	4	44	theme	fungal	852:857	arg1	mycelium					859:866	fungal mycelium	852:866	fungal mycelium	852:866	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	5	45	theme	MPa	1162:1164	arg1	values					1119:1124	values	1119:1124	values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom)	1119:1175	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	6	46	theme	nanopapers	1438:1447	arg1	mechanical					1387:1396	mechanical	1387:1396	mechanical	1387:1396	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	3	47	theme	low-cost	532:539	arg1	growth					490:495	Fungal growth	483:495	Fungal growth	483:495	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	47	theme	low-cost	532:539	arg1	method					541:546	a low-cost method	530:546	a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments	530:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	48	theme	microfilaments	714:727	arg1	form					699:702	the form	695:702	the form of fungal microfilaments	695:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	5	49	from	presence	1250:1257	arg1	nanopapers					1292:1301	the nanopapers	1288:1301	the nanopapers	1288:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	50	theme	existing	1085:1092	arg1	materials					1103:1111	the existing mycelium materials	1081:1111	the existing mycelium materials	1081:1111	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	51	dep	properties	1220:1229	arg1	addition					1181:1188	addition	1181:1188	addition	1181:1188	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	52	theme	filamentous	126:136	arg1	fungi					138:142	filamentous fungi	126:142	filamentous fungi	126:142	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	5	53	dep	∼25	1135:1137	arg1	to					1132:1133	to	1132:1133	to	1132:1133	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	54	theme	mycelium	1094:1101	arg1	materials					1103:1111	the existing mycelium materials	1081:1111	the existing mycelium materials	1081:1111	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	4	55	theme	various	833:839	arg1	species					841:847	various species	833:847	various species of fungal mycelium grown on sugarcane byproduct molasses	833:904	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	1	56	theme	fungi	138:142	arg1	Mycelium					91:98	Mycelium	91:98	Mycelium	91:98	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	1	56	theme	fungi	138:142	arg1	growth					116:121	the vegetative growth	101:121	the vegetative growth of filamentous fungi	101:142	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	3	57	attach	isolated	649:656	arg2	chitosan					616:623	chitosan	616:623	chitosan	616:623	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	57	attach	isolated	649:656	arg2	chitin					605:610	chitin	605:610	chitin	605:610	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	57	attach	isolated	649:656	arg1	byproducts					681:690	byproducts	681:690	byproducts in the form of fungal microfilaments	681:727	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	57	attach	isolated	649:656	arg1	wastes					670:675	liquid wastes	663:675	liquid wastes	663:675	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	3	57	attach	isolated	649:656	arg2	nanofibrils					584:594	natural nanofibrils	576:594	natural nanofibrils	576:594	Fungal growth can be alternatively utilized as a low-cost method for on-demand generation of natural nanofibrils, such as chitin and chitosan, which can be grown and isolated from liquid wastes and byproducts in the form of fungal microfilaments.
31355634	4	58	theme	mushroom	810:817	arg1	reference					819:827	a common mushroom reference	801:827	a common mushroom reference	801:827	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	7	59	theme	wide	1499:1502	arg1	coatings					1536:1543	coatings	1536:1543	coatings	1536:1543	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	59	theme	wide	1499:1502	arg1	membranes					1546:1554	membranes	1546:1554	membranes	1546:1554	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	59	theme	wide	1499:1502	arg1	range					1504:1508	a wide range	1497:1508	a wide range of applications including coatings, membranes, packaging, and paper	1497:1576	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	59	theme	wide	1499:1502	arg1	paper					1572:1576	paper	1572:1576	paper	1572:1576	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	7	59	theme	wide	1499:1502	arg1	packaging					1557:1565	packaging	1557:1565	packaging	1557:1565	This potentially enables their use in a wide range of applications including coatings, membranes, packaging, and paper.
31355634	1	60	from	interest	194:201	arg1	years					213:217	recent years	206:217	recent years	206:217	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	4	61	theme	polymer	755:761	arg1	extracts					763:770	polymer extracts	755:770	polymer extracts	755:770	This study characterized polymer extracts and nanopapers produced from a common mushroom reference and various species of fungal mycelium grown on sugarcane byproduct molasses.
31355634	2	62	theme	mycelium	345:352	arg1	composites					354:363	mycelium composites	345:363	mycelium composites	345:363	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	5	63	link	crustacean-derived	981:998	arg1	chitin					1000:1005	crustacean-derived chitin	981:1005	crustacean-derived chitin	981:1005	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	1	64	theme	recent	206:211	arg1	years					213:217	recent years	206:217	recent years	206:217	Mycelium, the vegetative growth of filamentous fungi, has attracted increasing commercial and academic interest in recent years because of its ability to upcycle agricultural and industrial wastes into low-cost, sustainable composite materials.
31355634	5	65	theme	%	931:931	arg1	yields					915:920	Polymer yields	907:920	Polymer yields of ∼10-26%	907:931	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	0	66	theme	Surface	71:77	arg1	Properties					79:88	Tunable Mechanical and Surface Properties	48:88	Properties	79:88	Waste-Derived Low-Cost Mycelium Nanopapers with Tunable Mechanical and Surface Properties.
31355634	5	67	theme	higher	1051:1056	arg1	strengths					1066:1074	much higher tensile strengths	1046:1074	much higher tensile strengths	1046:1074	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	5	68	theme	surface	1212:1218	arg1	properties					1220:1229	useful hydrophobic surface properties	1193:1229	useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers	1193:1301	Polymer yields of ∼10-26% were achieved, which are comparable to those of crustacean-derived chitin, and the nanopapers produced exhibited much higher tensile strengths than the existing mycelium materials, with values of up to ∼25 MPa (mycelium) and ∼98 MPa (mushroom), in addition to useful hydrophobic surface properties resulting from the presence of organic lipid residues in the nanopapers.
31355634	2	69	theme	mechanical	393:402	arg1	properties					404:413	foam-like mechanical properties	383:413	foam-like mechanical properties	383:413	However, mycelium composites typically exhibit foam-like mechanical properties, primarily originating from their weak organic filler constituents.
31355634	6	70	used	used	1332:1335	arg2	HCl					1304:1306	HCl	1304:1306	HCl	1304:1306	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
31355634	6	70	used	used	1332:1335	arg2	H2O2					1311:1314	H2O2	1311:1314	H2O2	1311:1314	HCl or H2O2 treatments were used to remove these impurities facilitating tuning of mechanical, thermal, and surface properties of the nanopapers produced.
29975387	4	0	theme	α-l-Araf	870:877	arg1	backbone					822:829	a backbone	820:829	a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp	820:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	6	1	theme	inhibitory	1251:1260	arg1	activity					1262:1269	outstanding α-glucosidase inhibitory activity	1225:1269	outstanding α-glucosidase inhibitory activity	1225:1269	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	3	2	theme	resonance	750:758	arg1	NMR					774:776	NMR	774:776	NMR	774:776	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	2	theme	resonance	750:758	arg1	spectroscopy					760:771	nuclear magnetic resonance spectroscopy	733:771	nuclear magnetic resonance spectroscopy (NMR)	733:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	6	3	theme	outstanding	1225:1235	arg1	activity					1262:1269	outstanding α-glucosidase inhibitory activity	1225:1269	outstanding α-glucosidase inhibitory activity	1225:1269	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	3	4	theme	nuclear	733:739	arg1	resonance					750:758	nuclear magnetic resonance	733:758	nuclear magnetic resonance spectroscopy (NMR)	733:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	5	theme	first	552:556	arg1	time					558:561	the first time	548:561	the first time	548:561	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	4	6	link	-linked	862:868	arg1	α-l-Araf					870:877	1→5)-linked α-l-Araf	858:877	1→5)-linked α-l-Araf	858:877	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	7	from	treatment	257:265	arg1	China					291:295	China	291:295	China	291:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	3	8	theme	composition	581:591	arg1	spectroscopy					627:638	Fourier transform-infrared spectroscopy	600:638	Fourier transform-infrared spectroscopy (FT-IR)	600:646	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	8	theme	composition	581:591	arg1	analysis					661:668	methylation analysis	649:668	methylation analysis	649:668	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	8	theme	composition	581:591	arg1	chromatography					675:688	gas chromatography	671:688	gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR)	671:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	8	theme	composition	581:591	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	1	9	theme	fruit	192:196	arg1	food					210:213	a subtropical fruit, functional food	178:213	a subtropical fruit, functional food	178:213	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	1	9	theme	fruit	192:196	arg1	fruit					169:173	Psidium guajava fruit	153:173	Psidium guajava fruit	153:173	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	7	10	theme	glucose	1558:1564	arg1	level					1566:1570	the postprandial blood glucose level	1535:1570	the postprandial blood glucose level	1535:1570	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	4	11	theme	β-d-Galp	917:924	arg1	backbone					822:829	a backbone	820:829	a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp	820:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	0	12	from	application	72:82	arg1	guava					58:62	green guava	52:62	green guava	52:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	7	13	theme	postprandial	1539:1550	arg1	level					1566:1570	the postprandial blood glucose level	1535:1570	the postprandial blood glucose level	1535:1570	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	4	14	theme	-linked	884:890	arg1	β-d-Galp					892:899	1→6)-linked β-d-Galp	880:899	1→6)-linked β-d-Galp	880:899	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	15	theme	transform-infrared	608:625	arg1	spectroscopy					627:638	Fourier transform-infrared spectroscopy	600:638	Fourier transform-infrared spectroscopy (FT-IR)	600:646	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	15	theme	transform-infrared	608:625	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	15	theme	transform-infrared	608:625	arg1	FT-IR					641:645	FT-IR	641:645	FT-IR	641:645	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	6	16	theme	α-glucosidase	1237:1249	arg1	activity					1262:1269	outstanding α-glucosidase inhibitory activity	1225:1269	outstanding α-glucosidase inhibitory activity	1225:1269	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	5	17	theme	irregular	1151:1159	arg1	flakes					1161:1166	irregular flakes	1151:1166	irregular flakes with rounded-spherical pores	1151:1195	Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores.
29975387	3	18	theme	methylation	649:659	arg1	analysis					661:668	methylation analysis	649:668	methylation analysis	649:668	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	18	theme	methylation	649:659	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	4	19	theme	-linked	949:955	arg1	β-d-GalpA					1046:1054	1→3)-linked β-d-GalpA	1034:1054	1→3)-linked β-d-GalpA	1034:1054	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	19	theme	-linked	949:955	arg1	β-d-GlcpA					1023:1031	1→3)-linked β-d-GlcpA	1011:1031	1→3)-linked β-d-GlcpA	1011:1031	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	19	theme	-linked	949:955	arg1	α-l-Rhap					1001:1008	1→3)-linked α-l-Rhap	989:1008	1→3)-linked α-l-Rhap	989:1008	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	19	theme	-linked	949:955	arg1	α-l-Araf					979:986	1→3)-linked α-l-Araf	967:986	1→3)-linked α-l-Araf	967:986	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	19	theme	-linked	949:955	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	20	theme	-linked	1079:1085	arg1	β-d-Galp					1087:1094	→1)-linked β-d-Galp	1076:1094	→1)-linked β-d-Galp	1076:1094	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	21	dep	β-d-Galp	892:899	arg1	1→6					880:882	1→6	880:882	1→6	880:882	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	22	link	-linked	993:999	arg1	α-l-Rhap					1001:1008	1→3)-linked α-l-Rhap	989:1008	1→3)-linked α-l-Rhap	989:1008	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	22	link	-linked	993:999	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	23	theme	Psidium	153:159	arg1	food					210:213	a subtropical fruit, functional food	178:213	a subtropical fruit, functional food	178:213	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	1	23	theme	Psidium	153:159	arg1	fruit					169:173	Psidium guajava fruit	153:173	Psidium guajava fruit	153:173	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	0	24	from	Characterization	0:15	arg1	guava					58:62	green guava	52:62	green guava	52:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	2	25	theme	heteropolysaccharide	380:399	arg1	GP70-3					401:406	a novel heteropolysaccharide GP70-3	372:406	a novel heteropolysaccharide GP70-3	372:406	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	6	26	theme	2.539	1303:1307	arg1	μM					1317:1318	2.539 ± 0.144 μM	1303:1318	2.539 ± 0.144 μM	1303:1318	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	2	27	theme	ethanol	442:448	arg1	precipitation					450:462	ethanol precipitation	442:462	ethanol precipitation	442:462	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	6	28	theme	positive	1366:1373	arg1	acarbose					1383:1390	the positive control acarbose	1362:1390	the positive control acarbose (IC50 value of 4.744 ± 0.026 mM)	1362:1423	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	5	29	theme	structure	1106:1114	arg1	studies					1116:1122	Advanced structure studies	1097:1122	Advanced structure studies	1097:1122	Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores.
29975387	4	30	dep	β-d-GlcpA	1023:1031	arg1	1→3					1011:1013	1→3	1011:1013	1→3	1011:1013	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	31	theme	-linked	993:999	arg1	α-l-Rhap					1001:1008	1→3)-linked α-l-Rhap	989:1008	1→3)-linked α-l-Rhap	989:1008	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	31	theme	-linked	993:999	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	32	theme	mellitus	279:286	arg1	treatment					257:265	the adjuvant treatment	244:265	the adjuvant treatment of diabetes mellitus in China	244:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	4	33	link	-linked	949:955	arg1	β-d-GalpA					1046:1054	1→3)-linked β-d-GalpA	1034:1054	1→3)-linked β-d-GalpA	1034:1054	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	33	link	-linked	949:955	arg1	β-d-GlcpA					1023:1031	1→3)-linked β-d-GlcpA	1011:1031	1→3)-linked β-d-GlcpA	1011:1031	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	33	link	-linked	949:955	arg1	α-l-Rhap					1001:1008	1→3)-linked α-l-Rhap	989:1008	1→3)-linked α-l-Rhap	989:1008	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	33	link	-linked	949:955	arg1	α-l-Araf					979:986	1→3)-linked α-l-Araf	967:986	1→3)-linked α-l-Araf	967:986	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	33	link	-linked	949:955	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	34	link	-linked	1079:1085	arg1	β-d-Galp					1087:1094	→1)-linked β-d-Galp	1076:1094	→1)-linked β-d-Galp	1076:1094	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	0	35	theme	heteropolysaccharide	26:45	arg1	application					72:82	its application	68:82	its application as an α-glucosidase inhibitor for the treatment of type II diabetes	68:150	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	0	35	theme	heteropolysaccharide	26:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of a new heteropolysaccharide from green guava	0:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	4	36	theme	-linked	840:846	arg1	β-d-Galp					848:855	1→3,6)-linked β-d-Galp	834:855	1→3,6)-linked β-d-Galp	834:855	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	5	37	with	flakes	1161:1166	arg1	pores					1191:1195	rounded-spherical pores	1173:1195	rounded-spherical pores	1173:1195	Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores.
29975387	2	38	theme	responsible	335:345	arg1	components					324:333	the active components	313:333	the active components responsible for its health benefits	313:369	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	0	39	theme	green	52:56	arg1	guava					58:62	green guava	52:62	green guava	52:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	2	40	theme	active	317:322	arg1	components					324:333	the active components	313:333	the active components responsible for its health benefits	313:369	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	1	41	theme	traditional	219:229	arg1	medicine					231:238	traditional medicine	219:238	traditional medicine	219:238	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	6	42	theme	higher	1342:1347	arg1	times					1336:1340	1867 times	1331:1340	1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM)	1331:1423	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	4	43	dep	α-l-Araf	979:986	arg1	1→3					967:969	1→3	967:969	1→3	967:969	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	44	theme	GP70-3	522:527	arg1	characterization					502:517	Structural characterization	491:517	Structural characterization of GP70-3	491:527	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	6	45	theme	IC50	1393:1396	arg1	value					1398:1402	IC50 value	1393:1402	IC50 value of 4.744 ± 0.026 mM	1393:1422	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	4	46	theme	-linked	862:868	arg1	α-l-Araf					870:877	1→5)-linked α-l-Araf	858:877	1→5)-linked α-l-Araf	858:877	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	47	from	China	291:295	arg1	treatment					257:265	the adjuvant treatment	244:265	the adjuvant treatment of diabetes mellitus in China	244:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	1	48	theme	adjuvant	248:255	arg1	treatment					257:265	the adjuvant treatment	244:265	the adjuvant treatment of diabetes mellitus in China	244:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	4	49	link	-linked	884:890	arg1	β-d-Galp					892:899	1→6)-linked β-d-Galp	880:899	1→6)-linked β-d-Galp	880:899	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	50	from	mellitus	279:286	arg1	China					291:295	China	291:295	China	291:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	4	51	link	-linked	1015:1021	arg1	β-d-GlcpA					1023:1031	1→3)-linked β-d-GlcpA	1011:1031	1→3)-linked β-d-GlcpA	1011:1031	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	51	link	-linked	1015:1021	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	52	theme	guajava	161:167	arg1	food					210:213	a subtropical fruit, functional food	178:213	a subtropical fruit, functional food	178:213	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	1	52	theme	guajava	161:167	arg1	fruit					169:173	Psidium guajava fruit	153:173	Psidium guajava fruit	153:173	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	4	53	theme	β-d-Galp	848:855	arg1	backbone					822:829	a backbone	820:829	a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp	820:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	54	theme	magnetic	741:748	arg1	resonance					750:758	nuclear magnetic resonance	733:758	nuclear magnetic resonance spectroscopy (NMR)	733:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	4	55	dep	contained	810:818	arg1	branched					927:934	branched	927:934	branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA	927:1054	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	55	dep	contained	810:818	arg1	terminated					1060:1069	terminated	1060:1069	terminated with →1)-linked β-d-Galp	1060:1094	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	56	theme	-linked	1038:1044	arg1	β-d-GalpA					1046:1054	1→3)-linked β-d-GalpA	1034:1054	1→3)-linked β-d-GalpA	1034:1054	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	56	theme	-linked	1038:1044	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	57	theme	monosaccharide	566:579	arg1	spectroscopy					627:638	Fourier transform-infrared spectroscopy	600:638	Fourier transform-infrared spectroscopy (FT-IR)	600:646	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	57	theme	monosaccharide	566:579	arg1	analysis					661:668	methylation analysis	649:668	methylation analysis	649:668	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	57	theme	monosaccharide	566:579	arg1	chromatography					675:688	gas chromatography	671:688	gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR)	671:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	57	theme	monosaccharide	566:579	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	1	58	theme	subtropical	180:190	arg1	food					210:213	a subtropical fruit, functional food	178:213	a subtropical fruit, functional food	178:213	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	1	58	theme	subtropical	180:190	arg1	fruit					169:173	Psidium guajava fruit	153:173	Psidium guajava fruit	153:173	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	7	59	theme	blood	1552:1556	arg1	level					1566:1570	the postprandial blood glucose level	1535:1570	the postprandial blood glucose level	1535:1570	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	0	60	theme	type	135:138	arg1	diabetes					143:150	type II diabetes	135:150	type II diabetes	135:150	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	6	61	theme	mM	1421:1422	arg1	value					1398:1402	IC50 value	1393:1402	IC50 value of 4.744 ± 0.026 mM	1393:1422	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	4	62	theme	-linked	909:915	arg1	β-d-Galp					917:924	1→3)-linked β-d-Galp	905:924	1→3)-linked β-d-Galp	905:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	63	dep	β-d-Galp	917:924	arg1	1→3					905:907	1→3	905:907	1→3	905:907	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	0	64	theme	diabetes	143:150	arg1	treatment					122:130	the treatment	118:130	the treatment of type II diabetes	118:150	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	3	65	theme	Fourier	600:606	arg1	spectroscopy					627:638	Fourier transform-infrared spectroscopy	600:638	Fourier transform-infrared spectroscopy (FT-IR)	600:646	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	65	theme	Fourier	600:606	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	65	theme	Fourier	600:606	arg1	FT-IR					641:645	FT-IR	641:645	FT-IR	641:645	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	4	66	dep	β-d-GalpA	1046:1054	arg1	1→3					1034:1036	1→3	1034:1036	1→3	1034:1036	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	7	67	theme	α-glucosidase	1498:1510	arg1	inhibitor					1512:1520	an α-glucosidase inhibitor	1495:1520	an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes	1495:1600	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	4	68	theme	β-d-Galp	892:899	arg1	backbone					822:829	a backbone	820:829	a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp	820:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	69	contain	contained	810:818	arg1	GP70-3					803:808	GP70-3	803:808	GP70-3	803:808	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	69	contain	contained	810:818	arg2	backbone					822:829	a backbone	820:829	a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp	820:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	0	70	theme	α-glucosidase	90:102	arg1	inhibitor					104:112	an α-glucosidase inhibitor	87:112	an α-glucosidase inhibitor for the treatment of type II diabetes	87:150	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	7	71	theme	polysaccharides	1458:1472	arg1	consumption					1437:1447	consumption	1437:1447	consumption of guava polysaccharides	1437:1472	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	0	72	from	guava	58:62	arg1	application					72:82	its application	68:82	its application as an α-glucosidase inhibitor for the treatment of type II diabetes	68:150	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	0	72	from	guava	58:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of a new heteropolysaccharide from green guava	0:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	0	72	from	guava	58:62	arg1	heteropolysaccharide					26:45	a new heteropolysaccharide	20:45	a new heteropolysaccharide from green guava	20:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	6	73	theme	μM	1317:1318	arg1	value					1294:1298	an IC50 value	1286:1298	an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM)	1286:1423	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	6	74	theme	±	1309:1309	arg1	μM					1317:1318	2.539 ± 0.144 μM	1303:1318	2.539 ± 0.144 μM	1303:1318	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	4	75	dep	α-l-Rhap	1001:1008	arg1	1→3					989:991	1→3	989:991	1→3	989:991	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	76	link	-linked	840:846	arg1	β-d-Galp					848:855	1→3,6)-linked β-d-Galp	834:855	1→3,6)-linked β-d-Galp	834:855	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	77	link	-linked	971:977	arg1	α-l-Araf					979:986	1→3)-linked α-l-Araf	967:986	1→3)-linked α-l-Araf	967:986	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	77	link	-linked	971:977	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	2	78	theme	novel	374:378	arg1	GP70-3					401:406	a novel heteropolysaccharide GP70-3	372:406	a novel heteropolysaccharide GP70-3	372:406	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	6	79	theme	control	1375:1381	arg1	acarbose					1383:1390	the positive control acarbose	1362:1390	the positive control acarbose (IC50 value of 4.744 ± 0.026 mM)	1362:1423	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	6	80	theme	IC50	1289:1292	arg1	value					1294:1298	an IC50 value	1286:1298	an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM)	1286:1423	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	0	81	theme	new	22:24	arg1	heteropolysaccharide					26:45	a new heteropolysaccharide	20:45	a new heteropolysaccharide from green guava	20:62	Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes.
29975387	1	82	theme	diabetes	270:277	arg1	mellitus					279:286	diabetes mellitus	270:286	diabetes mellitus in China	270:295	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	2	83	theme	water	424:428	arg1	extraction					430:439	water extraction	424:439	water extraction	424:439	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	3	84	theme	gas	671:673	arg1	chromatography					675:688	gas chromatography	671:688	gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR)	671:777	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	84	theme	gas	671:673	arg1	assay					593:597	monosaccharide composition assay	566:597	monosaccharide composition assay	566:597	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	7	85	theme	guava	1452:1456	arg1	polysaccharides					1458:1472	guava polysaccharides	1452:1472	guava polysaccharides	1452:1472	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	7	86	theme	type	1585:1588	arg1	diabetes					1593:1600	type II diabetes	1585:1600	type II diabetes	1585:1600	Therefore, consumption of guava polysaccharides may be beneficial as an α-glucosidase inhibitor for reducing the postprandial blood glucose level and treating type II diabetes.
29975387	2	87	theme	column	468:473	arg1	chromatography					475:488	column chromatography	468:488	column chromatography	468:488	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
29975387	4	88	dep	α-l-Araf	870:877	arg1	1→5					858:860	1→5	858:860	1→5	858:860	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	6	89	dep	acarbose	1383:1390	arg1	value					1398:1402	IC50 value	1393:1402	IC50 value of 4.744 ± 0.026 mM	1393:1422	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	4	90	theme	-linked	971:977	arg1	α-l-Araf					979:986	1→3)-linked α-l-Araf	967:986	1→3)-linked α-l-Araf	967:986	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	90	theme	-linked	971:977	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	91	theme	mass	703:706	arg1	GC-MS					722:726	GC-MS	722:726	GC-MS	722:726	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	3	91	theme	mass	703:706	arg1	spectrometry					708:719	mass spectrometry	703:719	mass spectrometry (GC-MS)	703:727	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	6	92	theme	0.026	1415:1419	arg1	±					1413:1413	±	1413:1413	±	1413:1413	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	5	93	theme	Advanced	1097:1104	arg1	studies					1116:1122	Advanced structure studies	1097:1122	Advanced structure studies	1097:1122	Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores.
29975387	4	94	theme	-linked	1015:1021	arg1	β-d-GlcpA					1023:1031	1→3)-linked β-d-GlcpA	1011:1031	1→3)-linked β-d-GlcpA	1011:1031	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	94	theme	-linked	1015:1021	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	3	95	theme	Structural	491:500	arg1	characterization					502:517	Structural characterization	491:517	Structural characterization of GP70-3	491:527	Structural characterization of GP70-3 was elucidated for the first time by monosaccharide composition assay, Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
29975387	6	96	theme	4.744	1407:1411	arg1	±					1413:1413	±	1413:1413	±	1413:1413	Moreover, GP70-3 exhibited outstanding α-glucosidase inhibitory activity in vitro, with an IC50 value of 2.539 ± 0.144 μM, which was 1867 times higher than that of the positive control acarbose (IC50 value of 4.744 ± 0.026 mM).
29975387	5	97	theme	rounded-spherical	1173:1189	arg1	pores					1191:1195	rounded-spherical pores	1173:1195	rounded-spherical pores	1173:1195	Advanced structure studies showed GP70-3 consisted of irregular flakes with rounded-spherical pores.
29975387	4	98	link	-linked	909:915	arg1	β-d-Galp					917:924	1→3)-linked β-d-Galp	905:924	1→3)-linked β-d-Galp	905:924	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	1	99	dep	fruit	192:196	arg1	functional					199:208	functional	199:208	functional	199:208	Psidium guajava fruit is a subtropical fruit, functional food and traditional medicine for the adjuvant treatment of diabetes mellitus in China.
29975387	4	100	link	-linked	1038:1044	arg1	β-d-GalpA					1046:1054	1→3)-linked β-d-GalpA	1034:1054	1→3)-linked β-d-GalpA	1034:1054	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	4	100	link	-linked	1038:1044	arg1	α-l-Araf					957:964	1→2,3,5)-linked α-l-Araf	941:964	1→2,3,5)-linked α-l-Araf	941:964	The data revealed that GP70-3 contained a backbone of 1→3,6)-linked β-d-Galp, 1→5)-linked α-l-Araf, 1→6)-linked β-d-Galp and 1→3)-linked β-d-Galp, branched with 1→2,3,5)-linked α-l-Araf, 1→3)-linked α-l-Araf, 1→3)-linked α-l-Rhap, 1→3)-linked β-d-GlcpA, 1→3)-linked β-d-GalpA and terminated with →1)-linked β-d-Galp.
29975387	2	101	theme	health	355:360	arg1	benefits					362:369	its health benefits	351:369	its health benefits	351:369	To investigate the active components responsible for its health benefits, a novel heteropolysaccharide GP70-3 was purified by water extraction, ethanol precipitation and column chromatography.
31006977	10	0	theme	biosynthetic	1612:1623	arg1	pathway					1625:1631	the alginate biosynthetic pathway	1599:1631	the alginate biosynthetic pathway	1599:1631	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	10	1	from	strain	1669:1674	arg1	functional					1641:1650	functional	1641:1650	functional	1641:1650	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	6	2	theme	commercial	920:929	arg1	production					931:940	commercial production	920:940	commercial production of alginate	920:952	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	9	3	theme	biosynthesis	1464:1475	arg1	MucE					1433:1436	MucE	1433:1436	MucE	1433:1436	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	9	3	theme	biosynthesis	1464:1475	arg1	activator					1442:1450	an activator	1439:1450	an activator of alginate biosynthesis	1439:1475	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	0	4	theme	commercial	71:80	arg1	production					82:91	commercial production	71:91	commercial production of alginate	71:103	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.
31006977	8	5	with	injection	1204:1212	arg1	P. aeruginosa					1229:1241	wild-type P. aeruginosa	1219:1241	wild-type P. aeruginosa	1219:1241	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	0	6	theme	alginate	96:103	arg1	production					82:91	commercial production	71:91	commercial production of alginate	71:103	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.
31006977	7	7	theme	key	1027:1029	arg1	genes					1045:1049	five key pathogenicity genes	1022:1049	five key pathogenicity genes	1022:1049	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	1	8	used	used	163:166	arg2	Alginate					106:113	Alginate	106:113	Alginate	106:113	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	8	used	used	163:166	arg2	agent					181:185	a gelling agent	171:185	a gelling agent in foods, cosmetics and healthcare products	171:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	8	used	used	163:166	arg2	polysaccharide					131:144	an important polysaccharide	118:144	an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products	118:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	8	9	theme	Intraperitoneal	1128:1142	arg1	injection					1144:1152	Intraperitoneal injection	1128:1152	Intraperitoneal injection of mice with PGN5	1128:1170	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	6	10	theme	alginate	945:952	arg1	production					931:940	commercial production	920:940	commercial production of alginate	920:952	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	11	11	theme	genetic	1681:1687	arg1	versatility					1689:1699	The genetic versatility	1677:1699	The genetic versatility of P. aeruginosa	1677:1716	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	3	12	theme	alginate	542:549	arg1	sources					531:537	more reliable and customizable sources	500:537	more reliable and customizable sources of alginate	500:549	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	4	13	theme	alginate	582:589	arg1	production					591:600	alginate production	582:600	alginate production	582:600	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	9	14	theme	large	1374:1378	arg1	alginate					1391:1398	alginate	1391:1398	alginate	1391:1398	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	9	14	theme	large	1374:1378	arg1	amounts					1380:1386	large amounts	1374:1386	large amounts of alginate	1374:1398	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	3	15	theme	customizable	518:529	arg1	sources					531:537	more reliable and customizable sources	500:537	more reliable and customizable sources of alginate	500:549	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	4	16	theme	alginate-containing	676:694	arg1	biofilms					696:703	alginate-containing biofilms	676:703	alginate-containing biofilms	676:703	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	3	17	theme	alginates	434:442	arg1	biotechnological					409:424	biotechnological	409:424	biotechnological	409:424	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	9	18	dep	overexpression	1415:1428	arg1	response					1403:1410	response	1403:1410	response	1403:1410	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	8	19	theme	95	1255:1256	arg1	%					1257:1257	%	1257:1257	%	1257:1257	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	5	20	theme	immunocompromised	804:820	arg1	patients					822:829	immunocompromised patients	804:829	immunocompromised patients	804:829	However, P. aeruginosa is an opportunistic pathogen that can cause life-threatening infections in immunocompromised patients.
31006977	10	21	theme	modified	1660:1667	arg1	strain					1669:1674	this modified strain	1655:1674	this modified strain	1655:1674	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	1	22	theme	gelling	173:179	arg1	agent					181:185	a gelling agent	171:185	a gelling agent in foods, cosmetics and healthcare products	171:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	22	theme	gelling	173:179	arg1	polysaccharide					131:144	an important polysaccharide	118:144	an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products	118:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	9	23	theme	alginate	1391:1398	arg1	alginate					1391:1398	alginate	1391:1398	alginate	1391:1398	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	9	23	theme	alginate	1391:1398	arg1	amounts					1380:1386	large amounts	1374:1386	large amounts of alginate	1374:1398	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	3	24	dep	biotechnological	409:424	arg1	uses					426:429	uses	426:429	uses	426:429	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	7	25	theme	recombination	974:986	arg1	strategy					988:995	a homologous recombination strategy	961:995	a homologous recombination strategy	961:995	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	8	26	with	injection	1144:1152	arg1	PGN5					1167:1170	PGN5	1167:1170	PGN5	1167:1170	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	3	27	theme	ocean	362:366	arg1	temperature					368:378	ocean temperature	362:378	ocean temperature	362:378	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	8	28	theme	mice	1157:1160	arg1	injection					1144:1152	Intraperitoneal injection	1128:1152	Intraperitoneal injection of mice with PGN5	1128:1170	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	2	29	theme	brown	292:296	arg1	seaweed					298:304	brown seaweed	292:304	brown seaweed	292:304	Currently, all alginate used commercially is extracted from brown seaweed.
31006977	4	30	theme	common	631:636	arg1	bacterium					652:660	a common Gram-negative bacterium	629:660	a common Gram-negative bacterium that can form alginate-containing biofilms	629:703	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	4	30	theme	common	631:636	arg1	aeruginosa					617:626	Pseudomonas aeruginosa	605:626	Pseudomonas aeruginosa	605:626	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	8	31	theme	%	1257:1257	arg1	mortality					1259:1267	95% mortality	1255:1267	95% mortality	1255:1267	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	10	32	theme	wild-type	1558:1566	arg1	P. aeruginosa					1568:1580	wild-type P. aeruginosa	1558:1580	wild-type P. aeruginosa	1558:1580	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	11	33	theme	specific	1783:1790	arg1	compositions					1801:1812	specific chemical compositions	1783:1812	specific chemical compositions	1783:1812	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	11	34	with	alginates	1768:1776	arg1	compositions					1801:1812	specific chemical compositions	1783:1812	specific chemical compositions	1783:1812	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	11	34	with	alginates	1768:1776	arg1	properties					1827:1836	physical properties	1818:1836	physical properties	1818:1836	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	8	35	theme	PGN5	1320:1323	arg1	virulence					1307:1315	the systemic virulence	1294:1315	the systemic virulence of PGN5	1294:1323	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	0	36	theme	strain	34:39	arg1	Generation					0:9	Generation	0:9	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.	0:104	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.
31006977	11	37	theme	chemical	1792:1799	arg1	compositions					1801:1812	specific chemical compositions	1783:1812	specific chemical compositions	1783:1812	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	3	38	theme	specific	449:456	arg1	properties					458:467	specific properties	449:467	specific properties	449:467	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	0	39	theme	attenuated	23:32	arg1	strain					34:39	a highly attenuated strain	14:39	a highly attenuated strain of Pseudomonas aeruginosa	14:65	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.
31006977	3	40	theme	biotechnological	409:424	arg1	number					399:404	the increasing number	384:404	the increasing number of biotechnological uses of alginates with specific properties	384:467	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	3	40	theme	biotechnological	409:424	arg1	temperature					368:378	ocean temperature	362:378	ocean temperature	362:378	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	4	41	theme	Gram-negative	638:650	arg1	bacterium					652:660	a common Gram-negative bacterium	629:660	a common Gram-negative bacterium that can form alginate-containing biofilms	629:703	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	4	41	theme	Gram-negative	638:650	arg1	aeruginosa					617:626	Pseudomonas aeruginosa	605:626	Pseudomonas aeruginosa	605:626	An alternative to seaweed for alginate production is Pseudomonas aeruginosa, a common Gram-negative bacterium that can form alginate-containing biofilms.
31006977	3	42	theme	increasing	388:397	arg1	number					399:404	the increasing number	384:404	the increasing number of biotechnological uses of alginates with specific properties	384:467	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	8	43	theme	wild-type	1219:1227	arg1	P. aeruginosa					1229:1241	wild-type P. aeruginosa	1219:1241	wild-type P. aeruginosa	1219:1241	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	7	44	theme	marker-free	1103:1113	arg1	PGN5					1122:1125	the marker-free strain PGN5	1099:1125	the marker-free strain PGN5	1099:1125	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	3	45	theme	environmental	321:333	arg1	changes					335:341	environmental changes	321:341	environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties	321:467	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	8	46	theme	%	1185:1185	arg1	mortality					1187:1195	0% mortality	1184:1195	0% mortality	1184:1195	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	3	47	theme	emerging	482:489	arg1	need					491:494	an emerging need	479:494	an emerging need for more reliable and customizable sources of alginate	479:549	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	5	48	theme	opportunistic	735:747	arg1	pathogen					749:756	an opportunistic pathogen	732:756	an opportunistic pathogen that can cause life-threatening infections in immunocompromised patients	732:829	However, P. aeruginosa is an opportunistic pathogen that can cause life-threatening infections in immunocompromised patients.
31006977	5	48	theme	opportunistic	735:747	arg1	P. aeruginosa					715:727	P. aeruginosa	715:727	P. aeruginosa	715:727	However, P. aeruginosa is an opportunistic pathogen that can cause life-threatening infections in immunocompromised patients.
31006977	9	49	theme	MucE	1433:1436	arg1	overexpression					1415:1428	overexpression	1415:1428	overexpression of MucE, an activator of alginate biosynthesis	1415:1475	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
31006977	7	50	theme	strain	1115:1120	arg1	PGN5					1122:1125	the marker-free strain PGN5	1099:1125	the marker-free strain PGN5	1099:1125	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	11	51	theme	physical	1818:1825	arg1	properties					1827:1836	physical properties	1818:1836	physical properties	1818:1836	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	10	52	from	functional	1641:1650	arg1	strain					1669:1674	this modified strain	1655:1674	this modified strain	1655:1674	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	7	53	theme	P. aeruginosa	1060:1072	arg1	chromosome					1074:1083	the P. aeruginosa chromosome	1056:1083	the P. aeruginosa chromosome	1056:1083	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	3	54	with	alginates	434:442	arg1	properties					458:467	specific properties	449:467	specific properties	449:467	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	6	55	theme	P. aeruginosa	882:894	arg1	strain					896:901	a non-pathogenic P. aeruginosa strain	865:901	a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate	865:952	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	6	55	theme	P. aeruginosa	882:894	arg1	safe					911:914	safe	911:914	safe	911:914	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	1	56	theme	important	121:129	arg1	Alginate					106:113	Alginate	106:113	Alginate	106:113	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	56	theme	important	121:129	arg1	polysaccharide					131:144	an important polysaccharide	118:144	an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products	118:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	56	theme	important	121:129	arg1	agent					181:185	a gelling agent	171:185	a gelling agent in foods, cosmetics and healthcare products	171:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	7	57	theme	homologous	963:972	arg1	recombination					974:986	a homologous recombination	961:986	a homologous recombination strategy	961:995	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	8	58	theme	0	1184:1184	arg1	%					1185:1185	%	1185:1185	%	1185:1185	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	6	59	theme	non-pathogenic	867:880	arg1	strain					896:901	a non-pathogenic P. aeruginosa strain	865:901	a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate	865:952	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	6	59	theme	non-pathogenic	867:880	arg1	safe					911:914	safe	911:914	safe	911:914	Therefore, we sought to engineer a non-pathogenic P. aeruginosa strain that is safe for commercial production of alginate.
31006977	11	60	theme	P. aeruginosa	1704:1716	arg1	versatility					1689:1699	The genetic versatility	1677:1699	The genetic versatility of P. aeruginosa	1677:1716	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	1	61	theme	healthcare	211:220	arg1	products					222:229	healthcare products	211:229	healthcare products	211:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	5	62	theme	life-threatening	773:788	arg1	infections					790:799	life-threatening infections	773:799	life-threatening infections	773:799	However, P. aeruginosa is an opportunistic pathogen that can cause life-threatening infections in immunocompromised patients.
31006977	1	63	from	agent	181:185	arg1	products					222:229	healthcare products	211:229	healthcare products	211:229	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	63	from	agent	181:185	arg1	foods					190:194	foods	190:194	foods	190:194	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	1	63	from	agent	181:185	arg1	cosmetics					197:205	cosmetics	197:205	cosmetics	197:205	Alginate is an important polysaccharide that is commonly used as a gelling agent in foods, cosmetics and healthcare products.
31006977	3	64	theme	reliable	505:512	arg1	sources					531:537	more reliable and customizable sources	500:537	more reliable and customizable sources of alginate	500:549	However, with environmental changes such as increasing ocean temperature and the increasing number of biotechnological uses of alginates with specific properties, there is an emerging need for more reliable and customizable sources of alginate.
31006977	11	65	dep	biomedical	1871:1880	arg1	needs					1882:1886	needs	1882:1886	needs	1882:1886	The genetic versatility of P. aeruginosa will allow us to further engineer PGN5 to produce alginates with specific chemical compositions and physical properties to meet different industrial and biomedical needs.
31006977	10	66	theme	alginate	1603:1610	arg1	pathway					1625:1631	the alginate biosynthetic pathway	1599:1631	the alginate biosynthetic pathway	1599:1631	The alginate produced by PGN5 is structurally identical to alginate produced by wild-type P. aeruginosa, indicating that the alginate biosynthetic pathway remains functional in this modified strain.
31006977	0	67	theme	aeruginosa	56:65	arg1	strain					34:39	a highly attenuated strain	14:39	a highly attenuated strain of Pseudomonas aeruginosa	14:65	Generation of a highly attenuated strain of Pseudomonas aeruginosa for commercial production of alginate.
31006977	7	68	theme	pathogenicity	1031:1043	arg1	genes					1045:1049	five key pathogenicity genes	1022:1049	five key pathogenicity genes	1022:1049	Using a homologous recombination strategy, we sequentially deleted five key pathogenicity genes from the P. aeruginosa chromosome, resulting in the marker-free strain PGN5.
31006977	8	69	theme	systemic	1298:1305	arg1	virulence					1307:1315	the systemic virulence	1294:1315	the systemic virulence of PGN5	1294:1323	Intraperitoneal injection of mice with PGN5 resulted in 0% mortality, while injection with wild-type P. aeruginosa resulted in 95% mortality, providing evidence that the systemic virulence of PGN5 is highly attenuated.
31006977	9	70	theme	alginate	1455:1462	arg1	biosynthesis					1464:1475	alginate biosynthesis	1455:1475	alginate biosynthesis	1455:1475	Importantly, PGN5 produces large amounts of alginate in response to overexpression of MucE, an activator of alginate biosynthesis.
29589895	12	0	from	medium	2103:2108	arg1	biocompatible					2062:2074	biocompatible	2062:2074	biocompatible	2062:2074	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	6	1	theme	range	1138:1142	arg1	pores					1119:1123	interconnected pores	1104:1123	interconnected pores of 10-250 μm range	1104:1142	All scaffolds exhibited interconnected pores of 10-250 μm range.
29589895	9	2	theme	mouse	1459:1463	arg1	cells					1476:1480	mouse osteoblast cells	1459:1480	mouse osteoblast cells	1459:1480	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	4	3	theme	XRD	894:896	arg1	patterns					898:905	the XRD patterns	890:905	the XRD patterns	890:905	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	3	4	theme	microscopy	727:736	arg1	techniques					738:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	5	theme	Ca2+-mediated	568:580	arg1	crosslinking					582:593	Ca2+-mediated crosslinking	568:593	Ca2+-mediated crosslinking (chemical crosslinking)	568:617	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	5	theme	Ca2+-mediated	568:580	arg1	crosslinking					605:616	chemical crosslinking	596:616	chemical crosslinking	596:616	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	6	theme	scanning	709:716	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy	709:736	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	0	7	theme	Graphene	0:7	arg1	Tool					17:20	Graphene Oxide-A Tool	0:20	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.	0:138	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	12	8	from	biocompatible	2062:2074	arg1	medium					2103:2108	a biological medium	2090:2108	a biological medium	2090:2108	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	4	9	theme	crystalline	869:879	arg1	peaks					881:885	crystalline peaks	869:885	crystalline peaks	869:885	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	11	10	theme	osteoblasts	1870:1880	arg1	ability					1859:1865	the osteogenic ability	1844:1865	the osteogenic ability of osteoblasts	1844:1880	However, chemical crosslinking of SA-CS-Col-GO showed no effect on the osteogenic ability of osteoblasts.
29589895	2	11	theme	graphene	350:357	arg1	oxide					359:363	graphene oxide	350:363	graphene oxide (GO)	350:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	9	12	theme	various	1496:1502	arg1	scaffolds					1504:1512	various scaffolds	1496:1512	various scaffolds	1496:1512	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	12	13	dep	scaffolds	1956:1964	arg1	used					2120:2123	used	2120:2123	could be used for application in engineering bone tissues	2111:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	12	13	dep	scaffolds	1956:1964	arg1	preserved					1971:1979	preserved	1971:1979	preserved porous structure with elongated Col fibrils	1971:2023	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	5	14	link	crosslinked	1057:1067	arg1	scaffolds					1069:1077	chemically crosslinked scaffolds	1046:1077	chemically crosslinked scaffolds	1046:1077	GO scaffolds showed increased mechanical properties which were further increased for chemically crosslinked scaffolds.
29589895	8	15	theme	SA-CS-Col-GO	1265:1276	arg1	scaffold					1278:1285	the SA-CS-Col-GO scaffold	1261:1285	the SA-CS-Col-GO scaffold with or without chemical crosslinking	1261:1323	Moreover, the SA-CS-Col-GO scaffold with or without chemical crosslinking was more stable as compared to SA-CS or SA-CS-Col scaffolds when placed in aqueous solution.
29589895	8	15	theme	SA-CS-Col-GO	1265:1276	arg1	stable					1334:1339	stable	1334:1339	stable	1334:1339	Moreover, the SA-CS-Col-GO scaffold with or without chemical crosslinking was more stable as compared to SA-CS or SA-CS-Col scaffolds when placed in aqueous solution.
29589895	10	16	theme	significant	1665:1675	arg1	increase					1677:1684	a significant increase	1663:1684	a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites	1663:1774	These measurements showed a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites.
29589895	4	17	theme	ion	927:929	arg1	complex					931:937	polyelectrolyte ion complex	911:937	polyelectrolyte ion complex	911:937	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	2	18	theme	SA-CS-Col	299:307	arg1	scaffolds					326:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds	265:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO)	265:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	12	19	used	used	2120:2123	arg2	composites					2040:2049	these composites	2034:2049	these composites	2034:2049	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	12	20	theme	bone	2156:2159	arg1	tissues					2161:2167	engineering bone tissues	2144:2167	engineering bone tissues	2144:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	4	21	theme	SA-CS-Col	783:791	arg1	matrix					793:798	the SA-CS-Col matrix	779:798	the SA-CS-Col matrix	779:798	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	1	22	theme	biodegradable	153:165	arg1	scaffold					167:174	a biodegradable scaffold	151:174	a biodegradable scaffold	151:174	Developing a biodegradable scaffold remains a major challenge in bone tissue engineering.
29589895	2	23	dep	oxide	359:363	arg1	GO					366:367	GO	366:367	GO	366:367	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	0	24	theme	Crosslinking	56:67	arg1	Scaffolds					101:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	2	25	theme	novel	265:269	arg1	scaffolds					326:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds	265:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO)	265:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	1	26	from	challenge	192:200	arg1	engineering					217:227	bone tissue engineering	205:227	bone tissue engineering	205:227	Developing a biodegradable scaffold remains a major challenge in bone tissue engineering.
29589895	0	27	theme	Alginate-Chitosan-Collagen	74:99	arg1	Scaffolds					101:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	1	28	theme	major	186:190	arg1	challenge					192:200	a major challenge	184:200	a major challenge in bone tissue engineering	184:227	Developing a biodegradable scaffold remains a major challenge in bone tissue engineering.
29589895	2	29	theme	composite	316:324	arg1	scaffolds					326:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds	265:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO)	265:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	5	30	theme	increased	981:989	arg1	properties					1002:1011	increased mechanical properties	981:1011	increased mechanical properties which were further increased for chemically crosslinked scaffolds	981:1077	GO scaffolds showed increased mechanical properties which were further increased for chemically crosslinked scaffolds.
29589895	8	31	theme	aqueous	1400:1406	arg1	solution					1408:1415	aqueous solution	1400:1415	aqueous solution	1400:1415	Moreover, the SA-CS-Col-GO scaffold with or without chemical crosslinking was more stable as compared to SA-CS or SA-CS-Col scaffolds when placed in aqueous solution.
29589895	10	32	theme	SA-CS-Col-GO	1712:1723	arg1	scaffold					1725:1732	the SA-CS-Col-GO scaffold	1708:1732	the SA-CS-Col-GO scaffold	1708:1732	These measurements showed a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites.
29589895	0	33	theme	Tissue	120:125	arg1	Engineering					127:137	Bone Tissue Engineering	115:137	Bone Tissue Engineering	115:137	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	4	34	from	reduction	856:864	arg1	patterns					898:905	the XRD patterns	890:905	the XRD patterns	890:905	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	4	34	from	reduction	856:864	arg1	complex					931:937	polyelectrolyte ion complex	911:937	polyelectrolyte ion complex	911:937	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	9	35	theme	alizarin	1612:1619	arg1	staining					1627:1634	alizarin red S staining	1612:1634	alizarin red S staining	1612:1634	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	10	36	attach	attached	1696:1703	arg2	cells					1690:1694	cells	1690:1694	cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites	1690:1774	These measurements showed a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites.
29589895	10	36	attach	attached	1696:1703	arg1	scaffold					1725:1732	the SA-CS-Col-GO scaffold	1708:1732	the SA-CS-Col-GO scaffold	1708:1732	These measurements showed a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites.
29589895	7	37	theme	porosity/swelling	1213:1229	arg1	ratio					1231:1235	the porosity/swelling ratio	1209:1235	the porosity/swelling ratio	1209:1235	By increasing the crosslinking density with Ca2+, a decrease in the porosity/swelling ratio was observed.
29589895	9	38	theme	S	1625:1625	arg1	staining					1627:1634	alizarin red S staining	1612:1634	alizarin red S staining	1612:1634	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	6	39	theme	interconnected	1104:1117	arg1	pores					1119:1123	interconnected pores	1104:1123	interconnected pores of 10-250 μm range	1104:1142	All scaffolds exhibited interconnected pores of 10-250 μm range.
29589895	2	40	theme	alginate-chitosan-collagen	271:296	arg1	scaffolds					326:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds	265:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO)	265:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	3	41	theme	compressive	488:498	arg1	modulus					500:506	compressive modulus	488:506	compressive modulus	488:506	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	12	42	theme	free	1941:1944	arg1	scaffolds					1956:1964	free SA-CS-Col scaffolds	1941:1964	free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues	1941:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	3	43	theme	water	466:470	arg1	absorption					472:481	water absorption	466:481	water absorption	466:481	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	12	44	theme	potential	1910:1918	arg1	use					1920:1922	the potential use	1906:1922	the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues	1906:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	10	45	theme	SA-CS-Col	1755:1763	arg1	composites					1765:1774	SA-CS-Col composites	1755:1774	SA-CS-Col composites	1755:1774	These measurements showed a significant increase for cells attached to the SA-CS-Col-GO scaffold compared to SA-CS or SA-CS-Col composites.
29589895	9	46	theme	in	1429:1430	arg1	studies					1450:1456	in vitro biochemical studies	1429:1456	in vitro biochemical studies	1429:1456	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	5	47	dep	scaffolds	964:972	arg1	GO					961:962	GO	961:962	GO	961:962	GO scaffolds showed increased mechanical properties which were further increased for chemically crosslinked scaffolds.
29589895	3	48	theme	X-ray	680:684	arg1	XRD					699:701	XRD	699:701	XRD	699:701	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	48	theme	X-ray	680:684	arg1	diffraction					686:696	X-ray diffraction	680:696	X-ray diffraction (XRD)	680:702	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	4	49	theme	crosslinking	815:826	arg1	density					828:834	both crosslinking density	810:834	both crosslinking density	810:834	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	9	50	theme	biochemical	1438:1448	arg1	studies					1450:1456	in vitro biochemical studies	1429:1456	in vitro biochemical studies	1429:1456	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	7	51	theme	crosslinking	1163:1174	arg1	density					1176:1182	the crosslinking density	1159:1182	the crosslinking density	1159:1182	By increasing the crosslinking density with Ca2+, a decrease in the porosity/swelling ratio was observed.
29589895	2	52	theme	porous	380:385	arg1	structures					387:396	porous structures	380:396	porous structures	380:396	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	4	53	theme	peaks	881:885	arg1	reduction					856:864	the reduction	852:864	the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex	852:937	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	6	54	theme	μm	1135:1136	arg1	range					1138:1142	10-250 μm range	1128:1142	10-250 μm range	1128:1142	All scaffolds exhibited interconnected pores of 10-250 μm range.
29589895	9	55	theme	osteoblast	1465:1474	arg1	cells					1476:1480	mouse osteoblast cells	1459:1480	mouse osteoblast cells	1459:1480	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	2	56	theme	-based	309:314	arg1	scaffolds					326:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds	265:334	novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO)	265:368	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	3	57	theme	electron	718:725	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy	709:736	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	58	theme	chemical	596:603	arg1	crosslinking					582:593	Ca2+-mediated crosslinking	568:593	Ca2+-mediated crosslinking (chemical crosslinking)	568:617	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	3	58	theme	chemical	596:603	arg1	crosslinking					605:616	chemical crosslinking	596:616	chemical crosslinking	596:616	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	1	59	theme	bone	205:208	arg1	engineering					217:227	bone tissue engineering	205:227	bone tissue engineering	205:227	Developing a biodegradable scaffold remains a major challenge in bone tissue engineering.
29589895	3	60	theme	diffraction	686:696	arg1	techniques					738:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	8	61	theme	chemical	1303:1310	arg1	crosslinking					1312:1323	chemical crosslinking	1303:1323	chemical crosslinking	1303:1323	Moreover, the SA-CS-Col-GO scaffold with or without chemical crosslinking was more stable as compared to SA-CS or SA-CS-Col scaffolds when placed in aqueous solution.
29589895	1	62	theme	tissue	210:215	arg1	engineering					217:227	bone tissue engineering	205:227	bone tissue engineering	205:227	Developing a biodegradable scaffold remains a major challenge in bone tissue engineering.
29589895	3	63	dep	Fourier	645:651	arg1	transform					653:661	transform	653:661	transform infrared (FTIR)	653:677	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	9	64	dep	in	1429:1430	arg1	vitro					1432:1436	vitro	1432:1436	vitro	1432:1436	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	9	65	theme	cell	1532:1535	arg1	proliferation					1537:1549	cell proliferation	1532:1549	cell proliferation	1532:1549	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	3	66	theme	Fourier	645:651	arg1	techniques					738:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	12	67	from	application	2129:2139	arg1	tissues					2161:2167	engineering bone tissues	2144:2167	engineering bone tissues	2144:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	4	68	theme	polyelectrolyte	911:925	arg1	complex					931:937	polyelectrolyte ion complex	911:937	polyelectrolyte ion complex	911:937	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	12	69	theme	engineering	2144:2154	arg1	tissues					2161:2167	engineering bone tissues	2144:2167	engineering bone tissues	2144:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	0	70	theme	Free	69:72	arg1	Scaffolds					101:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	45:109	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	3	71	theme	Raman	638:642	arg1	techniques					738:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques	638:747	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	9	72	theme	MTT	1560:1562	arg1	assay					1564:1568	MTT assay	1560:1568	MTT assay	1560:1568	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	3	73	dep	infrared	663:670	arg1	FTIR					673:676	FTIR	673:676	FTIR	673:676	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	11	74	theme	SA-CS-Col-GO	1811:1822	arg1	crosslinking					1795:1806	chemical crosslinking	1786:1806	chemical crosslinking of SA-CS-Col-GO	1786:1822	However, chemical crosslinking of SA-CS-Col-GO showed no effect on the osteogenic ability of osteoblasts.
29589895	0	75	theme	Scaffolds	101:109	arg1	Preparation					30:40	the Preparation	26:40	the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds	26:109	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	8	76	theme	SA-CS-Col	1365:1373	arg1	scaffolds					1375:1383	SA-CS-Col scaffolds	1365:1383	SA-CS-Col scaffolds	1365:1383	Moreover, the SA-CS-Col-GO scaffold with or without chemical crosslinking was more stable as compared to SA-CS or SA-CS-Col scaffolds when placed in aqueous solution.
29589895	11	77	theme	osteogenic	1848:1857	arg1	ability					1859:1865	the osteogenic ability	1844:1865	the osteogenic ability of osteoblasts	1844:1880	However, chemical crosslinking of SA-CS-Col-GO showed no effect on the osteogenic ability of osteoblasts.
29589895	0	78	theme	Bone	115:118	arg1	Engineering					127:137	Bone Tissue Engineering	115:137	Bone Tissue Engineering	115:137	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	12	79	theme	Col	2013:2015	arg1	fibrils					2017:2023	elongated Col fibrils	2003:2023	elongated Col fibrils	2003:2023	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	7	80	from	decrease	1197:1204	arg1	ratio					1231:1235	the porosity/swelling ratio	1209:1235	the porosity/swelling ratio	1209:1235	By increasing the crosslinking density with Ca2+, a decrease in the porosity/swelling ratio was observed.
29589895	4	81	theme	GO	771:772	arg1	incorporation					754:766	The incorporation	750:766	The incorporation of GO into the SA-CS-Col matrix	750:798	The incorporation of GO into the SA-CS-Col matrix increased both crosslinking density as indicated by the reduction of crystalline peaks in the XRD patterns and polyelectrolyte ion complex as confirmed by FTIR.
29589895	3	82	dep	scaffolds	512:520	arg1	GO					509:510	GO	509:510	GO	509:510	To characterize porosity, water absorption, and compressive modulus, GO scaffolds (SA-CS-Col-GO) were prepared with and without Ca2+-mediated crosslinking (chemical crosslinking) and analyzed using Raman, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and scanning electron microscopy techniques.
29589895	0	83	theme	Oxide-A	9:15	arg1	Tool					17:20	Graphene Oxide-A Tool	0:20	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.	0:138	Graphene Oxide-A Tool for the Preparation of Chemically Crosslinking Free Alginate-Chitosan-Collagen Scaffolds for Bone Tissue Engineering.
29589895	12	84	theme	porous	1981:1986	arg1	structure					1988:1996	porous structure	1981:1996	porous structure	1981:1996	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	9	85	theme	red	1621:1623	arg1	staining					1627:1634	alizarin red S staining	1612:1634	alizarin red S staining	1612:1634	To perform in vitro biochemical studies, mouse osteoblast cells were grown on various scaffolds and evaluated for cell proliferation by using MTT assay and mineralization and differentiation by alizarin red S staining.
29589895	5	86	theme	mechanical	991:1000	arg1	properties					1002:1011	increased mechanical properties	981:1011	increased mechanical properties which were further increased for chemically crosslinked scaffolds	981:1077	GO scaffolds showed increased mechanical properties which were further increased for chemically crosslinked scaffolds.
29589895	12	87	theme	SA-CS-Col	1946:1954	arg1	scaffolds					1956:1964	free SA-CS-Col scaffolds	1941:1964	free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues	1941:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	2	88	theme	freeze-drying	415:427	arg1	technique					429:437	the freeze-drying technique	411:437	the freeze-drying technique	411:437	This study was aimed at developing novel alginate-chitosan-collagen (SA-CS-Col)-based composite scaffolds consisting of graphene oxide (GO) to enrich porous structures, elicited by the freeze-drying technique.
29589895	5	89	theme	crosslinked	1057:1067	arg1	scaffolds					1069:1077	chemically crosslinked scaffolds	1046:1077	chemically crosslinked scaffolds	1046:1077	GO scaffolds showed increased mechanical properties which were further increased for chemically crosslinked scaffolds.
29589895	11	90	theme	chemical	1786:1793	arg1	crosslinking					1795:1806	chemical crosslinking	1786:1806	chemical crosslinking of SA-CS-Col-GO	1786:1822	However, chemical crosslinking of SA-CS-Col-GO showed no effect on the osteogenic ability of osteoblasts.
29589895	12	91	theme	GO	1927:1928	arg1	use					1920:1922	the potential use	1906:1922	the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues	1906:2167	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	12	92	theme	elongated	2003:2011	arg1	fibrils					2017:2023	elongated Col fibrils	2003:2023	elongated Col fibrils	2003:2023	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
29589895	12	93	theme	biological	2092:2101	arg1	medium					2103:2108	a biological medium	2090:2108	a biological medium	2090:2108	These studies indicate the potential use of GO to prepare free SA-CS-Col scaffolds with preserved porous structure with elongated Col fibrils and that these composites, which are biocompatible and stable in a biological medium, could be used for application in engineering bone tissues.
30245679	0	0	theme	Halophile	74:82	arg1	sp					95:96	the Halophile Halorubrum sp	70:96	the Halophile Halorubrum sp	70:96	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	0	1	from	Sugars	17:22	arg1	Archaea					27:33	Archaea	27:33	Archaea	27:33	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	3	2	theme	N-linked	212:219	arg1	glycans					221:227	N-linked glycans	212:227	N-linked glycans	212:227	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	4	3	theme	legionaminic	444:455	arg1	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	3	theme	legionaminic	444:455	arg1	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	3	theme	legionaminic	444:455	arg1	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	3	theme	legionaminic	444:455	arg1	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	3	theme	legionaminic	444:455	arg1	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	12	4	theme	pathway	1613:1619	arg1	example					1569:1575	the first example	1559:1575	the first example of a legionaminic acid biosynthesis pathway in Archaea	1559:1630	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	10	5	theme	legionaminic	1228:1239	arg1	acid					1241:1244	legionaminic acid	1228:1244	legionaminic acid	1228:1244	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	10	6	theme	2,4-di-N-acetyl-6-deoxymannose	1068:1097	arg1	condensation					1052:1063	the condensation	1048:1063	the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid	1048:1151	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	10	7	theme	LegI	1026:1029	arg1	activities					1012:1021	the activities	1008:1021	the activities	1008:1021	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	12	8	from	example	1569:1575	arg1	Archaea					1624:1630	Archaea	1624:1630	Archaea	1624:1630	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	11	9	theme	genome	1327:1332	arg1	analysis					1334:1341	Further genome analysis	1319:1341	Further genome analysis	1319:1341	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	4	10	theme	pseudaminic	421:431	arg1	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	10	theme	pseudaminic	421:431	arg1	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	10	theme	pseudaminic	421:431	arg1	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	10	theme	pseudaminic	421:431	arg1	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	10	theme	pseudaminic	421:431	arg1	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	8	11	theme	sequences	814:822	arg1	identification					796:809	the identification	792:809	the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway	792:887	PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway.
30245679	5	12	theme	Halorubrum	680:689	arg1	sp					691:692	the haloarchaea Halorubrum sp	664:692	the haloarchaea Halorubrum sp	664:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	5	13	theme	N-linked	608:615	arg1	glycan					617:622	the N-linked glycan	604:622	the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp	604:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	12	14	theme	life	1780:1783	arg1	domain					1770:1775	this domain	1765:1775	this domain of life	1765:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	14	theme	life	1780:1783	arg1	life					1780:1783	life	1780:1783	life	1780:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	11	15	theme	pathway	1521:1527	arg1	components					1487:1496	components	1487:1496	components of the N-glycosylation pathway in this organism	1487:1544	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	0	16	theme	Halorubrum	84:93	arg1	sp					95:96	the Halophile Halorubrum sp	70:96	the Halophile Halorubrum sp	70:96	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	4	17	link	protein-linked	504:517	arg1	glycans					519:525	protein-linked glycans	504:525	protein-linked glycans	504:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	18	theme	nine-carbon	375:385	arg1	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	18	theme	nine-carbon	375:385	arg1	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	18	theme	nine-carbon	375:385	arg1	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	18	theme	nine-carbon	375:385	arg1	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	18	theme	nine-carbon	375:385	arg1	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	11	19	from	pathway	1521:1527	arg1	organism					1537:1544	this organism	1532:1544	this organism	1532:1544	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	10	20	theme	cytidine	1196:1203	arg1	CMP					1220:1222	CMP	1220:1222	CMP	1220:1222	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	10	20	theme	cytidine	1196:1203	arg1	monophosphate					1205:1217	cytidine monophosphate	1196:1217	cytidine monophosphate (CMP)	1196:1223	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	11	21	theme	acid	1404:1407	arg1	pathway					1422:1428	the legionaminic acid biosynthetic pathway	1387:1428	the legionaminic acid biosynthetic pathway	1387:1428	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	12	22	theme	archaeal	1684:1691	arg1	N-glycosylation					1693:1707	archaeal N-glycosylation	1684:1707	archaeal N-glycosylation	1684:1707	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	22	theme	archaeal	1684:1691	arg1	modification					1749:1760	an almost universal post-translational modification	1710:1760	an almost universal post-translational modification in this domain of life	1710:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	4	23	theme	nonulosonic	348:358	arg1	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	23	theme	nonulosonic	348:358	arg1	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	23	theme	nonulosonic	348:358	arg1	NulOs					367:371	NulOs	367:371	NulOs	367:371	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	23	theme	nonulosonic	348:358	arg1	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	23	theme	nonulosonic	348:358	arg1	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	23	theme	nonulosonic	348:358	arg1	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	11	24	theme	Further	1319:1325	arg1	analysis					1334:1341	Further genome analysis	1319:1341	Further genome analysis	1319:1341	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	11	25	theme	biosynthetic	1409:1420	arg1	pathway					1422:1428	the legionaminic acid biosynthetic pathway	1387:1428	the legionaminic acid biosynthetic pathway	1387:1428	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	10	26	theme	legionaminic	1135:1146	arg1	acid					1148:1151	legionaminic acid	1135:1151	legionaminic acid	1135:1151	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	0	27	theme	Acid-Like	7:15	arg1	Sugars					17:22	Sialic Acid-Like Sugars	0:22	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.	0:97	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	2	28	theme	post-translational	125:142	arg1	modification					144:155	a post-translational modification	123:155	a post-translational modification that occurs in all three domains	123:188	N-glycosylation is a post-translational modification that occurs in all three domains.
30245679	2	28	theme	post-translational	125:142	arg1	N-glycosylation					104:118	N-glycosylation	104:118	N-glycosylation	104:118	N-glycosylation is a post-translational modification that occurs in all three domains.
30245679	9	29	dep	co-transcription	951:966	arg1	was					943:945	was	943:945	was	943:945	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	9	29	dep	co-transcription	951:966	arg1	as					940:941	as	940:941	as	940:941	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	12	30	theme	biosynthesis	1600:1611	arg1	pathway					1613:1619	a legionaminic acid biosynthesis pathway	1580:1619	a legionaminic acid biosynthesis pathway	1580:1619	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	11	31	theme	pathway	1422:1428	arg1	enzymes					1376:1382	enzymes	1376:1382	enzymes of the legionaminic acid biosynthetic pathway	1376:1428	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	0	32	theme	Sialic	0:5	arg1	Sugars					17:22	Sialic Acid-Like Sugars	0:22	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.	0:97	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	7	33	theme	sp	770:771	arg1	Analysis					743:750	Analysis	743:750	Analysis of the Halorubrum sp.	743:772	Analysis of the Halorubrum sp.
30245679	12	34	theme	post-translational	1730:1747	arg1	N-glycosylation					1693:1707	archaeal N-glycosylation	1684:1707	archaeal N-glycosylation	1684:1707	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	34	theme	post-translational	1730:1747	arg1	modification					1749:1760	an almost universal post-translational modification	1710:1760	an almost universal post-translational modification in this domain of life	1710:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	11	35	from	components	1487:1496	arg1	organism					1537:1544	this organism	1532:1544	this organism	1532:1544	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	8	36	theme	acid	863:866	arg1	pathway					881:887	the legionaminic acid biosynthesis pathway	846:887	the legionaminic acid biosynthesis pathway	846:887	PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway.
30245679	4	37	theme	glycans	519:525	arg1	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	37	theme	glycans	519:525	arg1	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	37	theme	glycans	519:525	arg1	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	37	theme	glycans	519:525	arg1	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	37	theme	glycans	519:525	arg1	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	12	38	theme	legionaminic	1582:1593	arg1	pathway					1613:1619	a legionaminic acid biosynthesis pathway	1580:1619	a legionaminic acid biosynthesis pathway	1580:1619	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	5	39	attach	attached	624:631	arg2	glycan					617:622	the N-linked glycan	604:622	the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp	604:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	5	39	attach	attached	624:631	arg1	glycoprotein					648:659	the S-layer glycoprotein	636:659	the S-layer glycoprotein of the haloarchaea Halorubrum sp	636:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	5	40	link	N-linked	608:615	arg1	glycan					617:622	the N-linked glycan	604:622	the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp	604:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	9	41	theme	genes	919:923	arg1	transcription					894:906	The transcription	890:906	The transcription of pathway genes	890:923	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	10	42	theme	Haloferax	1280:1288	arg1	volcanii					1290:1297	Haloferax volcanii	1280:1297	Haloferax volcanii	1280:1297	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	11	43	from	organism	1537:1544	arg1	components					1487:1496	components	1487:1496	components of the N-glycosylation pathway in this organism	1487:1544	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	7	44	theme	Halorubrum	759:768	arg1	sp					770:771	the Halorubrum sp	755:771	the Halorubrum sp	755:771	Analysis of the Halorubrum sp.
30245679	3	45	theme	compositional	249:261	arg1	diversity					263:271	compositional diversity	249:271	compositional diversity not observed in either Eukarya or Bacteria	249:314	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	10	46	dep	catalyzes	1170:1178	arg1	both					1247:1250	both	1247:1250	both	1247:1250	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	5	47	theme	haloarchaea	668:678	arg1	sp					691:692	the haloarchaea Halorubrum sp	664:692	the haloarchaea Halorubrum sp	664:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	8	48	theme	biosynthesis	868:879	arg1	pathway					881:887	the legionaminic acid biosynthesis pathway	846:887	the legionaminic acid biosynthesis pathway	846:887	PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway.
30245679	3	49	link	N-linked	212:219	arg1	glycans					221:227	N-linked glycans	212:227	N-linked glycans	212:227	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	3	50	located	observed	277:284	arg1	Bacteria					307:314	Bacteria	307:314	Bacteria	307:314	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	3	50	located	observed	277:284	arg1	Eukarya					296:302	Eukarya	296:302	Eukarya	296:302	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	3	50	located	observed	277:284	arg2	diversity					263:271	compositional diversity	249:271	compositional diversity not observed in either Eukarya or Bacteria	249:314	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	3	51	theme	diversity	263:271	arg1	degree					239:244	a degree	237:244	a degree of compositional diversity not observed in either Eukarya or Bacteria	237:314	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	5	52	theme	sp	691:692	arg1	glycoprotein					648:659	the S-layer glycoprotein	636:659	the S-layer glycoprotein of the haloarchaea Halorubrum sp	636:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	10	53	theme	LegF	1158:1161	arg1	activities					1012:1021	the activities	1008:1021	the activities	1008:1021	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	6	54	theme	legionaminic	724:735	arg1	acid					737:740	an N-formylated legionaminic acid	708:740	an N-formylated legionaminic acid	708:740	PV6 includes an N-formylated legionaminic acid.
30245679	0	55	theme	Acid	49:52	arg1	Biosynthesis					54:65	Legionaminic Acid Biosynthesis	36:65	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.	0:97	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	9	56	theme	pathway	911:917	arg1	genes					919:923	pathway genes	911:923	pathway genes	911:923	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	0	57	from	Biosynthesis	54:65	arg1	sp					95:96	the Halophile Halorubrum sp	70:96	the Halophile Halorubrum sp	70:96	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	6	58	theme	N-formylated	711:722	arg1	acid					737:740	an N-formylated legionaminic acid	708:740	an N-formylated legionaminic acid	708:740	PV6 includes an N-formylated legionaminic acid.
30245679	11	59	theme	N-glycosylation	1505:1519	arg1	pathway					1521:1527	the N-glycosylation pathway	1501:1527	the N-glycosylation pathway in this organism	1501:1544	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	0	60	theme	Legionaminic	36:47	arg1	Biosynthesis					54:65	Legionaminic Acid Biosynthesis	36:65	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.	0:97	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	4	61	theme	protein-linked	504:517	arg1	glycans					519:525	protein-linked glycans	504:525	protein-linked glycans	504:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	11	62	theme	legionaminic	1391:1402	arg1	pathway					1422:1428	the legionaminic acid biosynthetic pathway	1387:1428	the legionaminic acid biosynthetic pathway	1387:1428	Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism.
30245679	5	63	gly	glycoprotein	648:659	arg1	glycoprotein					648:659	the S-layer glycoprotein	636:659	the S-layer glycoprotein of the haloarchaea Halorubrum sp	636:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	12	64	theme	universal	1720:1728	arg1	N-glycosylation					1693:1707	archaeal N-glycosylation	1684:1707	archaeal N-glycosylation	1684:1707	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	64	theme	universal	1720:1728	arg1	modification					1749:1760	an almost universal post-translational modification	1710:1760	an almost universal post-translational modification in this domain of life	1710:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	65	theme	acid	1595:1598	arg1	pathway					1613:1619	a legionaminic acid biosynthesis pathway	1580:1619	a legionaminic acid biosynthesis pathway	1580:1619	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	8	66	theme	PV6	774:776	arg1	genome					778:783	PV6 genome	774:783	PV6 genome	774:783	PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway.
30245679	12	67	theme	first	1563:1567	arg1	example					1569:1575	the first example	1559:1575	the first example of a legionaminic acid biosynthesis pathway in Archaea	1559:1630	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	5	68	theme	S-layer	640:646	arg1	glycoprotein					648:659	the S-layer glycoprotein	636:659	the S-layer glycoprotein of the haloarchaea Halorubrum sp	636:692	In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp.
30245679	4	69	theme	sialic	407:412	arg1	acids					414:418	sialic acids	407:418	sialic acids	407:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	0	70	dep	Sugars	17:22	arg1	Biosynthesis					54:65	Legionaminic Acid Biosynthesis	36:65	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.	0:97	Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp.
30245679	4	71	located	detected	478:485	arg2	acids					457:461	legionaminic acids	444:461	legionaminic acids	444:461	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg2	components					490:499	components	490:499	components of protein-linked glycans	490:525	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg2	acids					433:437	pseudaminic acids	421:437	pseudaminic acids	421:437	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg1	Eukarya					530:536	Eukarya	530:536	Eukarya	530:536	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg2	acids					360:364	nonulosonic acids	348:364	nonulosonic acids (NulOs)	348:372	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg2	sugars					387:392	nine-carbon sugars	375:392	nine-carbon sugars that include sialic acids	375:418	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	4	71	located	detected	478:485	arg2	NulOs					367:371	NulOs	367:371	NulOs	367:371	As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea.
30245679	10	72	theme	monophosphate	1205:1217	arg1	addition					1184:1191	the addition	1180:1191	the addition of cytidine monophosphate (CMP) to legionaminic acid	1180:1244	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	12	73	from	modification	1749:1760	arg1	domain					1770:1775	this domain	1765:1775	this domain of life	1765:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	12	73	from	modification	1749:1760	arg1	life					1780:1783	life	1780:1783	life	1780:1783	In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
30245679	10	74	theme	phosphoenolpyruvate	1103:1121	arg1	condensation					1052:1063	the condensation	1048:1063	the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid	1048:1151	In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated.
30245679	9	75	theme	genes	988:992	arg1	several					971:977	several	971:977	several	971:977	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	9	76	theme	several	971:977	arg1	co-transcription					951:966	the co-transcription	947:966	the co-transcription of several of these genes	947:992	The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes.
30245679	8	77	theme	legionaminic	850:861	arg1	pathway					881:887	the legionaminic acid biosynthesis pathway	846:887	the legionaminic acid biosynthesis pathway	846:887	PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway.
30245679	3	78	attach	present	229:235	arg1	Archaea					194:200	Archaea	194:200	Archaea	194:200	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
30245679	3	78	attach	present	229:235	arg2	glycans					221:227	N-linked glycans	212:227	N-linked glycans	212:227	In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria.
29953890	8	0	theme	pharmaceutical	1161:1174	arg1	applications					1176:1187	food and pharmaceutical applications	1152:1187	food and pharmaceutical applications	1152:1187	These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
29953890	4	1	theme	molar	533:537	arg1	ratio					539:543	a molar ratio	531:543	a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis	531:584	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	7	2	theme	CAT	1052:1054	arg1	activity					1056:1063	SOD, GSH-Px and CAT activity	1036:1063	SOD, GSH-Px and CAT activity	1036:1063	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	0	3	from	characterization	10:25	arg1	Walp.					71:75	Lepidium meyenii Walp.	54:75	Lepidium meyenii Walp.	54:75	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	5	4	theme	NMR	623:625	arg1	analysis					636:643	NMR spectrum analysis	623:643	NMR spectrum analysis	623:643	Methylation combined with 1D and 2D NMR spectrum analysis revealed that MP1 was an arabinogalactan.
29953890	0	5	theme	activity	98:105	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.	0:171	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	5	6	theme	spectrum	627:634	arg1	analysis					636:643	NMR spectrum analysis	623:643	NMR spectrum analysis	623:643	Methylation combined with 1D and 2D NMR spectrum analysis revealed that MP1 was an arabinogalactan.
29953890	5	7	dep	1D	613:614	arg1	analysis					636:643	NMR spectrum analysis	623:643	NMR spectrum analysis	623:643	Methylation combined with 1D and 2D NMR spectrum analysis revealed that MP1 was an arabinogalactan.
29953890	0	8	theme	antioxidant	86:96	arg1	activity					98:105	its antioxidant activity	82:105	its antioxidant activity	82:105	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	7	9	theme	GSH-Px	1041:1046	arg1	activity					1056:1063	SOD, GSH-Px and CAT activity	1036:1063	SOD, GSH-Px and CAT activity	1036:1063	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	6	10	theme	peroxidation	824:835	arg1	inhibition					837:846	lipid peroxidation inhibition	818:846	lipid peroxidation inhibition	818:846	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	6	11	theme	DPPH	745:748	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	1	12	theme	new	297:299	arg1	MP1					317:319	MP1	317:319	MP1	317:319	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	12	theme	new	297:299	arg1	polysaccharide					301:314	a new polysaccharide	295:314	a new polysaccharide (MP1)	295:320	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	0	13	theme	protective	111:120	arg1	effect					122:127	protective effect	111:127	protective effect	111:127	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	6	14	theme	lipid	818:822	arg1	peroxidation					824:835	lipid peroxidation	818:835	lipid peroxidation inhibition	818:846	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	1	15	theme	structural	177:186	arg1	characterization					188:203	The structural characterization	173:203	The structural characterization	173:203	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	15	theme	structural	177:186	arg1	activity					218:225	antioxidant activity	206:225	antioxidant activity	206:225	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	15	theme	structural	177:186	arg1	effect					242:247	protective effect	231:247	protective effect	231:247	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	6	16	theme	vitro	736:740	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	4	17	theme	PMP-HPLC-UV	565:575	arg1	analysis					577:584	PMP-HPLC-UV analysis	565:584	PMP-HPLC-UV analysis	565:584	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	4	18	from	galactose	518:526	arg1	ratio					539:543	a molar ratio	531:543	a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis	531:584	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	6	19	theme	chelating	807:815	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	7	20	theme	LDH	1012:1014	arg1	level					1016:1020	LDH level	1012:1020	LDH level	1012:1020	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	0	21	theme	Structure	0:8	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.	0:171	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	1	22	theme	polysaccharide	301:314	arg1	characterization					188:203	The structural characterization	173:203	The structural characterization	173:203	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	22	theme	polysaccharide	301:314	arg1	activity					218:225	antioxidant activity	206:225	antioxidant activity	206:225	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	22	theme	polysaccharide	301:314	arg1	effect					242:247	protective effect	231:247	protective effect	231:247	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	6	23	theme	Fe2+	802:805	arg1	chelating					807:815	Fe2+ chelating	802:815	Fe2+ chelating	802:815	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	1	24	theme	antioxidant	206:216	arg1	characterization					188:203	The structural characterization	173:203	The structural characterization	173:203	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	24	theme	antioxidant	206:216	arg1	activity					218:225	antioxidant activity	206:225	antioxidant activity	206:225	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	7	25	theme	RAW264.7	910:917	arg1	cells					919:923	RAW264.7 cells	910:923	RAW264.7 cells	910:923	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	7	26	theme	stable	965:970	arg1	viability					977:985	stable cell viability	965:985	stable cell viability	965:985	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	4	27	from	arabinose	504:512	arg1	ratio					539:543	a molar ratio	531:543	a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis	531:584	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	7	28	theme	cell	972:975	arg1	viability					977:985	stable cell viability	965:985	stable cell viability	965:985	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	6	29	theme	antioxidant	712:722	arg1	activity					724:731	a moderate antioxidant activity	701:731	a moderate antioxidant activity	701:731	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	8	30	theme	potential	1094:1102	arg1	utilization					1104:1114	the potential utilization	1090:1114	the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications	1090:1187	These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
29953890	6	31	theme	moderate	703:710	arg1	activity					724:731	a moderate antioxidant activity	701:731	a moderate antioxidant activity	701:731	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	3	32	theme	HPLC-ELSD	436:444	arg1	analysis					446:453	HPLC-ELSD analysis	436:453	HPLC-ELSD analysis	436:453	The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis.
29953890	6	33	dep	assays	867:872	arg1	in					742:743	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	8	34	theme	MP1	1119:1121	arg1	utilization					1104:1114	the potential utilization	1090:1114	the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications	1090:1187	These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
29953890	1	35	theme	protective	231:240	arg1	characterization					188:203	The structural characterization	173:203	The structural characterization	173:203	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	1	35	theme	protective	231:240	arg1	effect					242:247	protective effect	231:247	protective effect	231:247	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	0	36	theme	polysaccharide	34:47	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.	0:171	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	0	37	theme	H2O2-induced	137:148	arg1	injury					150:155	H2O2-induced injury	137:155	H2O2-induced injury	137:155	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	4	38	theme	2:1	548:550	arg1	ratio					539:543	a molar ratio	531:543	a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis	531:584	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	0	39	theme	meyenii	63:69	arg1	Walp.					71:75	Lepidium meyenii Walp.	54:75	Lepidium meyenii Walp.	54:75	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	3	40	theme	molecular	382:390	arg1	4.67 × 105 Da					419:431	4.67 × 105 Da	419:431	4.67 × 105 Da	419:431	The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis.
29953890	3	40	theme	molecular	382:390	arg1	weight					392:397	The molecular weight	378:397	The molecular weight	378:397	The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis.
29953890	0	41	from	Walp.	71:75	arg1	activity					98:105	its antioxidant activity	82:105	its antioxidant activity	82:105	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	0	41	from	Walp.	71:75	arg1	effect					122:127	protective effect	111:127	protective effect	111:127	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	0	41	from	Walp.	71:75	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.	0:171	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	0	41	from	Walp.	71:75	arg1	polysaccharide					34:47	one polysaccharide	30:47	one polysaccharide from Lepidium meyenii Walp.	30:75	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	6	42	theme	radicals	781:788	arg1	scavenging					790:799	superoxide and hydroxyl radicals scavenging	757:799	superoxide and hydroxyl radicals scavenging	757:799	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	0	43	theme	Lepidium	54:61	arg1	Walp.					71:75	Lepidium meyenii Walp.	54:75	Lepidium meyenii Walp.	54:75	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	1	44	theme	Lepidium	336:343	arg1	Walp					353:356	Lepidium meyenii Walp	336:356	Lepidium meyenii Walp	336:356	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	0	45	theme	RAW264.7	157:164	arg1	cells					166:170	RAW264.7 cells	157:170	RAW264.7 cells	157:170	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	4	46	theme	Monosaccharide	456:469	arg1	composition					471:481	Monosaccharide composition	456:481	Monosaccharide composition	456:481	Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis.
29953890	0	47	dep	polysaccharide	34:47	arg1	cells					166:170	RAW264.7 cells	157:170	RAW264.7 cells	157:170	Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.
29953890	6	48	theme	hydroxyl	772:779	arg1	radicals					781:788	superoxide and hydroxyl radicals	757:788	radicals	781:788	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	6	49	contain	possessed	691:699	arg2	activity					724:731	a moderate antioxidant activity	701:731	a moderate antioxidant activity	701:731	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	6	49	contain	possessed	691:699	arg1	MP1					687:689	MP1	687:689	MP1	687:689	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	1	50	theme	meyenii	345:351	arg1	Walp					353:356	Lepidium meyenii Walp	336:356	Lepidium meyenii Walp	336:356	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	6	51	theme	inhibition	837:846	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	7	52	theme	SOD	1036:1038	arg1	activity					1056:1063	SOD, GSH-Px and CAT activity	1036:1063	SOD, GSH-Px and CAT activity	1036:1063	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	6	53	theme	power	861:865	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	6	54	theme	scavenging	790:799	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	6	55	theme	superoxide	757:766	arg1	radicals					781:788	superoxide and hydroxyl radicals	757:788	radicals	781:788	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	7	56	theme	H2O2-induced	930:941	arg1	injury					943:948	H2O2-induced injury	930:948	H2O2-induced injury	930:948	MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity.
29953890	1	57	theme	H2O2-induced	257:268	arg1	cells					286:290	H2O2-induced injury RAW264.7 cells	257:290	H2O2-induced injury RAW264.7 cells	257:290	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	8	58	theme	food	1152:1155	arg1	applications					1176:1187	food and pharmaceutical applications	1152:1187	food and pharmaceutical applications	1152:1187	These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
29953890	6	59	theme	reducing	852:859	arg1	power					861:865	reducing power	852:865	reducing power	852:865	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	1	60	theme	injury	270:275	arg1	cells					286:290	H2O2-induced injury RAW264.7 cells	257:290	H2O2-induced injury RAW264.7 cells	257:290	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
29953890	6	61	theme	ABTS	751:754	arg1	assays					867:872	DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	745:872	vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays	736:872	MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays.
29953890	8	62	theme	natural	1128:1134	arg1	antioxidant					1136:1146	a natural antioxidant	1126:1146	a natural antioxidant for food and pharmaceutical applications	1126:1187	These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.
29953890	1	63	theme	RAW264.7	277:284	arg1	cells					286:290	H2O2-induced injury RAW264.7 cells	257:290	H2O2-induced injury RAW264.7 cells	257:290	The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp.
30274077	0	0	theme	seed	101:104	arg1	adhesion					111:118	seed cell adhesion	101:118	seed cell adhesion	101:118	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	5	1	theme	bone	729:732	arg1	BMSCs					758:762	BMSCs	758:762	BMSCs	758:762	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	5	1	theme	bone	729:732	arg1	cells					751:755	bone mesenchymal stem cells	729:755	bone mesenchymal stem cells (BMSCs)	729:763	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	9	2	theme	SAP/nHA/CTS	1269:1279	arg1	scaffold					1281:1288	a SAP/nHA/CTS scaffold	1267:1288	a SAP/nHA/CTS scaffold	1267:1288	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	5	3	theme	mesenchymal	734:744	arg1	BMSCs					758:762	BMSCs	758:762	BMSCs	758:762	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	5	3	theme	mesenchymal	734:744	arg1	cells					751:755	bone mesenchymal stem cells	729:755	bone mesenchymal stem cells (BMSCs)	729:763	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	2	4	from	scaffolds	303:311	arg1	poor					287:290	poor	287:290	poor	287:290	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	2	5	from	poor	287:290	arg1	scaffolds					303:311	nHA/CTS scaffolds	295:311	nHA/CTS scaffolds	295:311	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	0	6	theme	cell	106:109	arg1	adhesion					111:118	seed cell adhesion	101:118	seed cell adhesion	101:118	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	8	7	dep	report	1095:1100	arg1	promote					1160:1166	promote	1160:1166	promote the reconstruction of femoral condylar bone defects	1160:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	8	7	dep	report	1095:1100	arg1	increase					1133:1140	increase	1133:1140	increase cell adhesion	1133:1154	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	2	8	theme	cell	259:262	arg1	adhesion					264:271	cell adhesion	259:271	cell adhesion	259:271	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	5	9	theme	scaffold	811:818	arg1	properties					793:802	the mechanical properties	778:802	the mechanical properties of the scaffold	778:818	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	8	10	theme	defects	1212:1218	arg1	reconstruction					1172:1185	the reconstruction	1168:1185	the reconstruction of femoral condylar bone defects	1168:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	8	11	theme	bone	1207:1210	arg1	defects					1212:1218	femoral condylar bone defects	1190:1218	femoral condylar bone defects	1190:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	5	12	theme	cells	751:755	arg1	adhesion					717:724	the adhesion	713:724	the adhesion of bone mesenchymal stem cells (BMSCs)	713:763	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	8	13	theme	SAP/nHA/CTS	1107:1117	arg1	scaffolds					1119:1127	SAP/nHA/CTS scaffolds	1107:1127	SAP/nHA/CTS scaffolds	1107:1127	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	4	14	theme	self-assembling	574:588	arg1	peptide					590:596	self-assembling peptide	574:596	self-assembling peptide (SAP)	574:602	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	4	14	theme	self-assembling	574:588	arg1	material					607:614	a material	605:614	a material which is similar to the extracellular matrix	605:659	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	4	14	theme	self-assembling	574:588	arg1	similar					625:631	similar	625:631	similar	625:631	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	4	14	theme	self-assembling	574:588	arg1	SAP					599:601	SAP	599:601	SAP	599:601	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	2	15	theme	three-dimensional	349:365	arg1	environment					367:377	an ideal three-dimensional environment	340:377	an ideal three-dimensional environment for seed cells	340:392	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	6	16	used	used	854:857	arg2	scaffold					836:843	This composite scaffold	821:843	This composite scaffold	821:843	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	7	17	theme	mineral	1036:1042	arg1	density					1044:1050	bone mineral density	1031:1050	bone mineral density	1031:1050	Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests.
30274077	2	18	theme	ideal	343:347	arg1	environment					367:377	an ideal three-dimensional environment	340:377	an ideal three-dimensional environment for seed cells	340:392	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	0	19	theme	Self-assembling	0:14	arg1	peptide					16:22	Self-assembling peptide and nHA/CTS composite scaffolds	0:54	peptide	16:22	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	8	20	theme	femoral	1190:1196	arg1	defects					1212:1218	femoral condylar bone defects	1190:1218	femoral condylar bone defects	1190:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	8	21	theme	first	1089:1093	arg1	this					1077:1080	this	1077:1080	this	1077:1080	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	8	21	theme	first	1089:1093	arg1	report					1095:1100	the first report	1085:1100	the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects	1085:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	8	22	theme	condylar	1198:1205	arg1	defects					1212:1218	femoral condylar bone defects	1190:1218	femoral condylar bone defects	1190:1218	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	0	23	theme	nHA/CTS	28:34	arg1	scaffolds					46:54	Self-assembling peptide and nHA/CTS composite scaffolds	0:54	scaffolds	46:54	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	5	24	theme	mechanical	782:791	arg1	properties					793:802	the mechanical properties	778:802	the mechanical properties of the scaffold	778:818	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	8	25	theme	cell	1142:1145	arg1	adhesion					1147:1154	cell adhesion	1142:1154	cell adhesion	1142:1154	To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects.
30274077	6	26	theme	mouse	905:909	arg1	model					911:915	a mouse model	903:915	a mouse model	903:915	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	7	27	theme	bone	1031:1034	arg1	density					1044:1050	bone mineral density	1031:1050	bone mineral density	1031:1050	Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests.
30274077	6	28	theme	composite	826:834	arg1	scaffold					836:843	This composite scaffold	821:843	This composite scaffold	821:843	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	1	29	theme	Porous	121:126	arg1	scaffolds					128:136	Porous scaffolds	121:136	Porous scaffolds	121:136	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	3	30	theme	BTE	447:449	arg1	scaffolds					451:459	these BTE scaffolds	441:459	these BTE scaffolds	441:459	These deficiencies limit the applicability of these BTE scaffolds to repair bone defects.
30274077	5	31	theme	stem	746:749	arg1	BMSCs					758:762	BMSCs	758:762	BMSCs	758:762	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	5	31	theme	stem	746:749	arg1	cells					751:755	bone mesenchymal stem cells	729:755	bone mesenchymal stem cells (BMSCs)	729:763	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	4	32	theme	composite	528:536	arg1	scaffold					538:545	a composite scaffold	526:545	a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix	526:659	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	1	33	used	used	211:214	arg2	scaffolds					128:136	Porous scaffolds	121:136	Porous scaffolds	121:136	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	2	34	theme	seed	383:386	arg1	cells					388:392	seed cells	383:392	seed cells	383:392	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	0	35	theme	composite	36:44	arg1	scaffolds					46:54	Self-assembling peptide and nHA/CTS composite scaffolds	0:54	scaffolds	46:54	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	9	36	theme	novel	1299:1303	arg1	strategy					1317:1324	a novel prospective strategy	1297:1324	a novel prospective strategy for healing extensive bone defects	1297:1359	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	9	36	theme	novel	1299:1303	arg1	BTE					1257:1259	BTE	1257:1259	BTE using a SAP/nHA/CTS scaffold	1257:1288	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	5	37	theme	SAP/nHA/CTS	676:686	arg1	scaffolds					688:696	SAP/nHA/CTS scaffolds	676:696	SAP/nHA/CTS scaffolds	676:696	We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold.
30274077	9	38	theme	bone	1348:1351	arg1	defects					1353:1359	healing extensive bone defects	1330:1359	healing extensive bone defects	1330:1359	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	0	39	theme	bone	64:67	arg1	regeneration					69:80	bone regeneration	64:80	bone regeneration	64:80	Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion.
30274077	9	40	theme	prospective	1305:1315	arg1	strategy					1317:1324	a novel prospective strategy	1297:1324	a novel prospective strategy for healing extensive bone defects	1297:1359	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	9	40	theme	prospective	1305:1315	arg1	BTE					1257:1259	BTE	1257:1259	BTE using a SAP/nHA/CTS scaffold	1257:1288	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	3	41	theme	repair	464:469	arg1	defects					476:482	repair bone defects	464:482	repair bone defects	464:482	These deficiencies limit the applicability of these BTE scaffolds to repair bone defects.
30274077	6	42	theme	bone	888:891	arg1	defect					893:898	a femoral condylar bone defect	869:898	a femoral condylar bone defect	869:898	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	7	43	theme	microcomputerized	997:1013	arg1	tomography					1015:1024	microcomputerized tomography	997:1024	microcomputerized tomography	997:1024	Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests.
30274077	3	44	theme	bone	471:474	arg1	defects					476:482	repair bone defects	464:482	repair bone defects	464:482	These deficiencies limit the applicability of these BTE scaffolds to repair bone defects.
30274077	6	45	theme	condylar	879:886	arg1	defect					893:898	a femoral condylar bone defect	869:898	a femoral condylar bone defect	869:898	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	7	46	theme	H&E	983:985	arg1	staining					987:994	H&E staining	983:994	H&E staining	983:994	Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests.
30274077	3	47	theme	scaffolds	451:459	arg1	applicability					424:436	the applicability	420:436	the applicability of these BTE scaffolds to repair bone defects	420:482	These deficiencies limit the applicability of these BTE scaffolds to repair bone defects.
30274077	9	48	theme	healing	1330:1336	arg1	defects					1353:1359	healing extensive bone defects	1330:1359	healing extensive bone defects	1330:1359	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	9	49	theme	extensive	1338:1346	arg1	defects					1353:1359	healing extensive bone defects	1330:1359	healing extensive bone defects	1330:1359	Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
30274077	2	50	theme	nHA/CTS	295:301	arg1	scaffolds					303:311	nHA/CTS scaffolds	295:311	nHA/CTS scaffolds	295:311	However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells.
30274077	1	51	theme	bone	219:222	arg1	BTE					244:246	BTE	244:246	BTE	244:246	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	1	51	theme	bone	219:222	arg1	engineering					231:241	bone tissue engineering	219:241	bone tissue engineering (BTE)	219:247	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	6	52	from	repair	862:867	arg1	model					911:915	a mouse model	903:915	a mouse model	903:915	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	4	53	theme	extracellular	640:652	arg1	matrix					654:659	the extracellular matrix	636:659	the extracellular matrix	636:659	To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix.
30274077	1	54	theme	tissue	224:229	arg1	BTE					244:246	BTE	244:246	BTE	244:246	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	1	54	theme	tissue	224:229	arg1	engineering					231:241	bone tissue engineering	219:241	bone tissue engineering (BTE)	219:247	Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE).
30274077	6	55	theme	femoral	871:877	arg1	defect					893:898	a femoral condylar bone defect	869:898	a femoral condylar bone defect	869:898	This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model.
30274077	7	56	dep	staining	987:994	arg1	tests					1052:1056	tests	1052:1056	tests	1052:1056	Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests.
31426978	4	0	link	6-linked	515:522	arg1	α-D-Glcp					524:531	6-linked α-D-Glcp	515:531	6-linked α-D-Glcp	515:531	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	α-D-Glcp					499:506	4-linked α-D-Glcp	490:506	4-linked α-D-Glcp	490:506	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	1					487:487	1	487:487	1	487:487	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg1	backbone					456:463	The backbone	452:463	The backbone of CDA-0.05	452:475	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	1					537:537	1	537:537	1	537:537	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	1					509:509	1	509:509	1	509:509	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	4					512:512	4	512:512	4	512:512	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	α-D-Glcp					524:531	6-linked α-D-Glcp	515:531	6-linked α-D-Glcp	515:531	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	1	contain	contained	477:485	arg2	β-D-Galp					549:556	4-linked β-D-Galp	540:556	4-linked β-D-Galp	540:556	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	3	2	from	galactose	411:419	arg1	ratio					432:436	a molar ratio	424:436	a molar ratio of 96.4: 3.6	424:449	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	5	3	theme	test	659:662	arg1	results					664:670	Bioactivity test results	647:670	Bioactivity test results	647:670	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	4	4	theme	4-linked	540:547	arg1	1					487:487	1	487:487	1	487:487	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	4	theme	4-linked	540:547	arg1	β-D-Galp					549:556	4-linked β-D-Galp	540:556	4-linked β-D-Galp	540:556	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	7	5	theme	gut	1144:1146	arg1	bacteria					1148:1155	gut bacteria	1144:1155	gut bacteria	1144:1155	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	5	6	theme	Bacteroides	741:751	arg1	species					730:736	three species	724:736	three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis)	724:800	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	5	7	dep	Bacteroides	741:751	arg1	thetaiotaomicron					757:772	thetaiotaomicron	757:772	thetaiotaomicron	757:772	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	5	7	dep	Bacteroides	741:751	arg1	ovatus					778:783	B. ovatus	775:783	B. ovatus	775:783	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	5	7	dep	Bacteroides	741:751	arg1	fragilis					792:799	B. fragilis	789:799	B. fragilis	789:799	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	4	8	theme	6-linked	619:626	arg1	residues					637:644	6-linked α-D-Glcp residues	619:644	6-linked α-D-Glcp residues	619:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	8	theme	6-linked	619:626	arg1	1					613:613	1	613:613	1	613:613	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	3	9	theme	composition	338:348	arg1	analysis					350:357	The monosaccharide composition analysis	319:357	The monosaccharide composition analysis	319:357	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	2	10	contain	have	159:162	arg1	polysaccharides					139:153	polysaccharides	139:153	polysaccharides	139:153	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	2	10	contain	have	159:162	arg2	impact					164:169	impact	164:169	impact	164:169	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	7	11	theme	fibers	1082:1087	arg1	CDA-0.05					991:998	CDA-0.05	991:998	CDA-0.05	991:998	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	7	11	theme	fibers	1082:1087	arg1	part					1074:1077	part	1074:1077	part of fibers or drugs candidate to benefit human body by regulating gut bacteria	1074:1155	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	4	12	theme	CDA-0.05	468:475	arg1	backbone					456:463	The backbone	452:463	The backbone of CDA-0.05	452:475	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	7	13	dep	fibers	1082:1087	arg1	candidate					1098:1106	candidate	1098:1106	candidate	1098:1106	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	2	14	theme	7.96 kDa	260:267	arg1	weight					250:255	an average molecular weight	229:255	an average molecular weight of 7.96 kDa	229:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	7	15	theme	intestinal	1023:1032	arg1	homeostasis					1034:1044	intestinal homeostasis	1023:1044	intestinal homeostasis	1023:1044	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	2	16	with	CDA-0.05	215:222	arg1	weight					250:255	an average molecular weight	229:255	an average molecular weight of 7.96 kDa	229:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	4	17	link	6-linked	619:626	arg1	residues					637:644	6-linked α-D-Glcp residues	619:644	6-linked α-D-Glcp residues	619:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	17	link	6-linked	619:626	arg1	1					613:613	1	613:613	1	613:613	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	18	theme	1	613:613	arg1	C-6					606:608	C-6	606:608	C-6 of 1, 4, 6-linked α-D-Glcp residues	606:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	19	theme	4-linked	490:497	arg1	α-D-Glcp					499:506	4-linked α-D-Glcp	490:506	4-linked α-D-Glcp	490:506	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	20	link	4-linked	490:497	arg1	α-D-Glcp					499:506	4-linked α-D-Glcp	490:506	4-linked α-D-Glcp	490:506	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	2	21	theme	neutral	192:198	arg1	CDA-0.05					215:222	a neutral polysaccharide CDA-0.05	190:222	a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa	190:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	3	22	theme	molar	426:430	arg1	ratio					432:436	a molar ratio	424:436	a molar ratio of 96.4: 3.6	424:449	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	2	23	theme	molecular	240:248	arg1	weight					250:255	an average molecular weight	229:255	an average molecular weight of 7.96 kDa	229:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	3	24	theme	3.6	447:449	arg1	ratio					432:436	a molar ratio	424:436	a molar ratio of 96.4: 3.6	424:449	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	2	25	theme	gut	174:176	arg1	microbiota					178:187	gut microbiota	174:187	gut microbiota	174:187	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	1	26	theme	intestinal	88:97	arg1	bacteria					99:106	intestinal bacteria	88:106	intestinal bacteria	88:106	and its bioactivity on intestinal bacteria strains.
31426978	4	27	theme	6-linked	515:522	arg1	α-D-Glcp					524:531	6-linked α-D-Glcp	515:531	6-linked α-D-Glcp	515:531	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	7	28	theme	human	1119:1123	arg1	body					1125:1128	human body	1119:1128	human body	1119:1128	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	7	29	theme	drugs	1092:1096	arg1	CDA-0.05					991:998	CDA-0.05	991:998	CDA-0.05	991:998	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	7	29	theme	drugs	1092:1096	arg1	part					1074:1077	part	1074:1077	part of fibers or drugs candidate to benefit human body by regulating gut bacteria	1074:1155	These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
31426978	5	30	theme	Bioactivity	647:657	arg1	results					664:670	Bioactivity test results	647:670	Bioactivity test results	647:670	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	4	31	theme	α-D-Glcp	585:592	arg1	branches					564:571	branches	564:571	branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues	564:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	5	32	theme	species	730:736	arg1	growth					714:719	the growth	710:719	the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis)	710:800	Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly.
31426978	6	33	theme	probiotics	863:872	arg1	growth					874:879	some probiotics growth	858:879	some probiotics growth	858:879	Furthermore, CDA-0.05 could also promote some probiotics growth, such as Lactobacillus casei, Lactobacillus plantarum and Lactobacillus reuteri.
31426978	4	34	link	4-linked	540:547	arg1	1					487:487	1	487:487	1	487:487	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	34	link	4-linked	540:547	arg1	β-D-Galp					549:556	4-linked β-D-Galp	540:556	4-linked β-D-Galp	540:556	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	2	35	theme	polysaccharide	200:213	arg1	CDA-0.05					215:222	a neutral polysaccharide CDA-0.05	190:222	a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa	190:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
31426978	4	36	theme	α-D-Glcp	628:635	arg1	residues					637:644	6-linked α-D-Glcp residues	619:644	6-linked α-D-Glcp residues	619:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	36	theme	α-D-Glcp	628:635	arg1	1					613:613	1	613:613	1	613:613	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	37	attach	attached	594:601	arg2	branches					564:571	branches	564:571	branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues	564:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	37	attach	attached	594:601	arg3	C-6					606:608	C-6	606:608	C-6 of 1, 4, 6-linked α-D-Glcp residues	606:644	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	38	link	T-linked	576:583	arg1	α-D-Glcp					585:592	T-linked α-D-Glcp	576:592	T-linked α-D-Glcp	576:592	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	4	39	theme	T-linked	576:583	arg1	α-D-Glcp					585:592	T-linked α-D-Glcp	576:592	T-linked α-D-Glcp	576:592	The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues.
31426978	1	40	from	bioactivity	73:83	arg1	bacteria					99:106	intestinal bacteria	88:106	intestinal bacteria	88:106	and its bioactivity on intestinal bacteria strains.
31426978	3	41	theme	monosaccharide	323:336	arg1	analysis					350:357	The monosaccharide composition analysis	319:357	The monosaccharide composition analysis	319:357	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	3	42	from	glucose	399:405	arg1	ratio					432:436	a molar ratio	424:436	a molar ratio of 96.4: 3.6	424:449	The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6.
31426978	2	43	theme	average	232:238	arg1	weight					250:255	an average molecular weight	229:255	an average molecular weight of 7.96 kDa	229:267	To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma.
30851325	6	0	theme	anionic	527:533	arg1	EPS-A28					513:519	EPS-A28	513:519	EPS-A28	513:519	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	6	0	theme	anionic	527:533	arg1	heteropolysaccharide					535:554	an anionic heteropolysaccharide	524:554	an anionic heteropolysaccharide with a molecular weight of 780 kDa	524:589	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	7	1	theme	Monosaccharide	651:664	arg1	composition					666:676	Monosaccharide composition	651:676	Monosaccharide composition	651:676	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	14	2	from	polymer	1479:1485	arg1	care					1496:1499	wound care	1490:1499	wound care	1490:1499	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	8	3	theme	acid	845:848	arg1	residues					850:857	uronic acid residues	838:857	uronic acid residues	838:857	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	9	4	dep	800 °C	971:976	arg1	up					965:966	up	965:966	up	965:966	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	6	5	theme	helical	612:618	arg1	structure					620:628	triple helical structure	605:628	triple helical structure in aqueous solution	605:648	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	2	6	theme	biopolymers	277:287	arg1	bacteria					145:152	Marine bacteria	138:152	Marine bacteria	138:152	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	2	6	theme	biopolymers	277:287	arg1	source					251:256	a source	249:256	a source of newer bioactive biopolymers	249:287	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	1	7	from	PRIM-28	67:73	arg1	healing					120:126	wound healing	114:126	wound healing	114:126	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	1	7	from	PRIM-28	67:73	arg1	vitro					131:135	vitro	131:135	vitro	131:135	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	1	7	from	PRIM-28	67:73	arg1	proliferation					96:108	cell proliferation	91:108	cell proliferation	91:108	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	7	8	theme	glucosamine	719:729	arg1	units					741:745	glucose and N-acetyl glucosamine repeating units	698:745	glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93	698:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	7	8	theme	glucosamine	719:729	arg1	acid					692:695	mannuronic acid	681:695	mannuronic acid	681:695	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	12	9	theme	proliferative	1147:1159	arg1	property					1161:1168	The proliferative property	1143:1168	The proliferative property of the EPS-A28	1143:1183	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	2	10	theme	bioactive	267:275	arg1	biopolymers					277:287	newer bioactive biopolymers	261:287	newer bioactive biopolymers	261:287	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	7	11	theme	repeating	731:739	arg1	units					741:745	glucose and N-acetyl glucosamine repeating units	698:745	glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93	698:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	7	11	theme	repeating	731:739	arg1	acid					692:695	mannuronic acid	681:695	mannuronic acid	681:695	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	9	12	theme	residues	944:951	arg1	residues					944:951	residues	944:951	residues	944:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	9	12	theme	residues	944:951	arg1	%					939:939	190 °C and 40%	926:939	190 °C and 40% of residues	926:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	2	13	theme	Marine	138:143	arg1	source					251:256	a source	249:256	a source of newer bioactive biopolymers	249:287	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	2	13	theme	Marine	138:143	arg1	bacteria					145:152	Marine bacteria	138:152	Marine bacteria	138:152	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	14	14	theme	bioactive	1469:1477	arg1	EPS-A28					1416:1422	EPS-A28	1416:1422	EPS-A28	1416:1422	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	14	14	theme	bioactive	1469:1477	arg1	polymer					1479:1485	a multifunctional bioactive polymer	1451:1485	a multifunctional bioactive polymer in wound care	1451:1499	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	2	15	theme	newer	261:265	arg1	biopolymers					277:287	newer bioactive biopolymers	261:287	newer bioactive biopolymers	261:287	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	5	16	theme	standard	440:447	arg1	methods					449:455	standard methods	440:455	standard methods	440:455	The EPS was characterised using standard methods and tested for its bioactivities using in vitro models.
30851325	3	17	theme	marine	330:335	arg1	bacterium					337:345	a marine bacterium	328:345	a marine bacterium identified as Alteromonas sp	328:374	This study reports an EPS produced by a marine bacterium identified as Alteromonas sp.
30851325	0	18	from	bacteria	42:49	arg1	exopolysaccharide					12:28	A bioactive exopolysaccharide	0:28	A bioactive exopolysaccharide from marine bacteria	0:49	A bioactive exopolysaccharide from marine bacteria Alteromonas sp.
30851325	14	19	theme	multifunctional	1453:1467	arg1	EPS-A28					1416:1422	EPS-A28	1416:1422	EPS-A28	1416:1422	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	14	19	theme	multifunctional	1453:1467	arg1	polymer					1479:1485	a multifunctional bioactive polymer	1451:1485	a multifunctional bioactive polymer in wound care	1451:1499	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	5	20	theme	in	496:497	arg1	models					505:510	in vitro models	496:510	in vitro models	496:510	The EPS was characterised using standard methods and tested for its bioactivities using in vitro models.
30851325	0	21	theme	bioactive	2:10	arg1	exopolysaccharide					12:28	A bioactive exopolysaccharide	0:28	A bioactive exopolysaccharide from marine bacteria	0:49	A bioactive exopolysaccharide from marine bacteria Alteromonas sp.
30851325	10	22	theme	keratinocytes	1078:1090	arg1	proliferation					1018:1030	proliferation	1018:1030	proliferation	1018:1030	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	10	22	theme	keratinocytes	1078:1090	arg1	migration					1036:1044	migration	1036:1044	migration	1036:1044	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	12	23	theme	EPS-A28	1177:1183	arg1	property					1161:1168	The proliferative property	1143:1168	The proliferative property of the EPS-A28	1143:1183	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	12	24	theme	marker	1257:1262	arg1	Ki-67					1265:1269	fibroblast proliferation marker (Ki-67)	1232:1270	fibroblast proliferation marker (Ki-67)	1232:1270	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	7	25	theme	mannuronic	681:690	arg1	units					741:745	glucose and N-acetyl glucosamine repeating units	698:745	glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93	698:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	7	25	theme	mannuronic	681:690	arg1	acid					692:695	mannuronic acid	681:695	mannuronic acid	681:695	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	12	26	theme	binding	1295:1301	arg1	capability					1281:1290	its capability	1277:1290	its capability of binding to cell surface	1277:1317	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	9	27	theme	thermal	864:870	arg1	analysis					872:879	The thermal analysis	860:879	The thermal analysis	860:879	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	1	28	theme	cell	91:94	arg1	proliferation					96:108	cell proliferation	91:108	cell proliferation	91:108	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	14	29	theme	wound	1490:1494	arg1	care					1496:1499	wound care	1490:1499	wound care	1490:1499	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	3	30	theme	Alteromonas	361:371	arg1	sp					373:374	Alteromonas sp	361:374	Alteromonas sp	361:374	This study reports an EPS produced by a marine bacterium identified as Alteromonas sp.
30851325	12	31	theme	Ki-67	1265:1269	arg1	expression					1218:1227	the increased expression	1204:1227	the increased expression of fibroblast proliferation marker (Ki-67)	1204:1270	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	12	32	theme	fibroblast	1232:1241	arg1	Ki-67					1265:1269	fibroblast proliferation marker (Ki-67)	1232:1270	fibroblast proliferation marker (Ki-67)	1232:1270	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	7	33	from	units	741:745	arg1	1:3.67:0.93					760:770	the ratio 1:3.67:0.93	750:770	the ratio 1:3.67:0.93	750:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	7	34	theme	N-acetyl	710:717	arg1	glucosamine					719:729	N-acetyl glucosamine	710:729	N-acetyl glucosamine	710:729	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	0	35	dep	sp	63:64	arg1	exopolysaccharide					12:28	A bioactive exopolysaccharide	0:28	A bioactive exopolysaccharide from marine bacteria	0:49	A bioactive exopolysaccharide from marine bacteria Alteromonas sp.
30851325	14	36	used	used	1443:1446	arg2	polymer					1479:1485	a multifunctional bioactive polymer	1451:1485	a multifunctional bioactive polymer in wound care	1451:1499	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	14	36	used	used	1443:1446	arg2	EPS-A28					1416:1422	EPS-A28	1416:1422	EPS-A28	1416:1422	These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.
30851325	0	37	theme	marine	35:40	arg1	bacteria					42:49	marine bacteria	35:49	marine bacteria	35:49	A bioactive exopolysaccharide from marine bacteria Alteromonas sp.
30851325	9	38	theme	partial	888:894	arg1	degradation					896:906	partial degradation	888:906	partial degradation of the EPS-A28 at 190 °C and 40% of residues	888:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	9	39	from	%	939:939	arg1	degradation					896:906	partial degradation	888:906	partial degradation of the EPS-A28 at 190 °C and 40% of residues	888:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	9	39	from	%	939:939	arg1	EPS-A28					915:921	the EPS-A28	911:921	the EPS-A28 at 190 °C and 40% of residues	911:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	13	40	theme	nitric	1336:1341	arg1	oxide					1343:1347	nitric oxide	1336:1347	nitric oxide	1336:1347	It also induced nitric oxide and arginase synthesis in macrophages.
30851325	5	41	dep	in	496:497	arg1	vitro					499:503	vitro	499:503	vitro	499:503	The EPS was characterised using standard methods and tested for its bioactivities using in vitro models.
30851325	6	42	theme	molecular	563:571	arg1	weight					573:578	a molecular weight	561:578	a molecular weight of 780 kDa	561:589	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	13	43	theme	arginase	1353:1360	arg1	synthesis					1362:1370	arginase synthesis	1353:1370	arginase synthesis	1353:1370	It also induced nitric oxide and arginase synthesis in macrophages.
30851325	7	44	theme	glucose	698:704	arg1	units					741:745	glucose and N-acetyl glucosamine repeating units	698:745	glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93	698:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	7	44	theme	glucose	698:704	arg1	acid					692:695	mannuronic acid	681:695	mannuronic acid	681:695	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
30851325	2	45	theme	functional	214:223	arg1	properties					225:234	unique structural and functional properties	192:234	unique structural and functional properties	192:234	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	8	46	theme	uronic	838:843	arg1	residues					850:857	uronic acid residues	838:857	uronic acid residues	838:857	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	2	47	with	exopolysaccharides	162:179	arg1	properties					225:234	unique structural and functional properties	192:234	unique structural and functional properties	192:234	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	0	48	theme	Alteromonas	51:61	arg1	sp					63:64	Alteromonas sp	51:64	Alteromonas sp	51:64	A bioactive exopolysaccharide from marine bacteria Alteromonas sp.
30851325	12	49	dep	established	1189:1199	arg1	capability					1281:1290	its capability	1277:1290	its capability of binding to cell surface	1277:1317	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	2	50	theme	structural	199:208	arg1	properties					225:234	unique structural and functional properties	192:234	unique structural and functional properties	192:234	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	10	51	theme	dermal	1049:1054	arg1	HDF					1069:1071	HDF	1069:1071	HDF	1069:1071	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	10	51	theme	dermal	1049:1054	arg1	fibroblasts					1056:1066	dermal fibroblasts	1049:1066	dermal fibroblasts (HDF)	1049:1072	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	12	52	theme	proliferation	1243:1255	arg1	Ki-67					1265:1269	fibroblast proliferation marker (Ki-67)	1232:1270	fibroblast proliferation marker (Ki-67)	1232:1270	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	1	53	theme	wound	114:118	arg1	healing					120:126	wound healing	114:126	wound healing	114:126	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	8	54	theme	phosphate	824:832	arg1	presence					802:809	the presence	798:809	the presence of sulphate, phosphate and uronic acid residues	798:857	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	6	55	from	structure	620:628	arg1	solution					641:648	aqueous solution	633:648	aqueous solution	633:648	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	2	56	theme	unique	192:197	arg1	properties					225:234	unique structural and functional properties	192:234	unique structural and functional properties	192:234	Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers.
30851325	6	57	with	heteropolysaccharide	535:554	arg1	weight					573:578	a molecular weight	561:578	a molecular weight of 780 kDa	561:589	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	8	58	theme	FT-IR	777:781	arg1	spectra					783:789	The FT-IR spectra	773:789	The FT-IR spectra	773:789	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	9	59	theme	EPS-A28	915:921	arg1	degradation					896:906	partial degradation	888:906	partial degradation of the EPS-A28 at 190 °C and 40% of residues	888:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	12	60	theme	cell	1306:1309	arg1	surface					1311:1317	cell surface	1306:1317	cell surface	1306:1317	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	11	61	theme	cycle	1136:1140	arg1	S-phase					1120:1126	the S-phase	1116:1126	the S-phase of cell cycle	1116:1140	EPS-A28 could increase the S-phase of cell cycle.
30851325	1	62	from	role	83:86	arg1	healing					120:126	wound healing	114:126	wound healing	114:126	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	1	62	from	role	83:86	arg1	vitro					131:135	vitro	131:135	vitro	131:135	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	1	62	from	role	83:86	arg1	proliferation					96:108	cell proliferation	91:108	cell proliferation	91:108	PRIM-28 and its role in cell proliferation and wound healing in vitro.
30851325	9	63	dep	showed	881:886	arg1	stable					958:963	stable	958:963	stable	958:963	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	6	64	theme	triple	605:610	arg1	structure					620:628	triple helical structure	605:628	triple helical structure in aqueous solution	605:648	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	11	65	theme	cell	1131:1134	arg1	cycle					1136:1140	cell cycle	1131:1140	cell cycle	1131:1140	EPS-A28 could increase the S-phase of cell cycle.
30851325	12	66	theme	increased	1208:1216	arg1	expression					1218:1227	the increased expression	1204:1227	the increased expression of fibroblast proliferation marker (Ki-67)	1204:1270	The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface.
30851325	6	67	theme	aqueous	633:639	arg1	solution					641:648	aqueous solution	633:648	aqueous solution	633:648	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	9	68	from	degradation	896:906	arg1	residues					944:951	residues	944:951	residues	944:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	9	68	from	degradation	896:906	arg1	%					939:939	190 °C and 40%	926:939	190 °C and 40% of residues	926:951	The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C.
30851325	8	69	theme	sulphate	814:821	arg1	presence					802:809	the presence	798:809	the presence of sulphate, phosphate and uronic acid residues	798:857	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	6	70	theme	780 kDa	583:589	arg1	weight					573:578	a molecular weight	561:578	a molecular weight of 780 kDa	561:589	EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution.
30851325	10	71	theme	fibroblasts	1056:1066	arg1	proliferation					1018:1030	proliferation	1018:1030	proliferation	1018:1030	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	10	71	theme	fibroblasts	1056:1066	arg1	migration					1036:1044	migration	1036:1044	migration	1036:1044	It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes.
30851325	8	72	theme	residues	850:857	arg1	presence					802:809	the presence	798:809	the presence of sulphate, phosphate and uronic acid residues	798:857	The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues.
30851325	7	73	theme	ratio	754:758	arg1	1:3.67:0.93					760:770	the ratio 1:3.67:0.93	750:770	the ratio 1:3.67:0.93	750:770	Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93.
31018583	0	0	theme	Polysaccharides	90:104	arg1	Purification					31:42	Purification	31:42	Purification	31:42	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	0	theme	Polysaccharides	90:104	arg1	Characterization					53:68	Partial Characterization	45:68	Partial Characterization	45:68	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	0	theme	Polysaccharides	90:104	arg1	Extraction					19:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	0	theme	Polysaccharides	90:104	arg1	Bioactivity					75:85	Bioactivity	75:85	Bioactivity	75:85	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	1	1	theme	activity	337:344	arg1	knowledge					307:315	knowledge	307:315	knowledge of the bactericidal activity of P. ginseng polysaccharides	307:374	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	4	2	theme	±	913:913	arg1	yields					895:900	the actual yields	884:900	the actual yields	884:900	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	2	theme	±	913:913	arg1	%					919:919	41.6% ± 0.09%	907:919	41.6% ± 0.09%	907:919	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	6	3	theme	105	1101:1103	arg1	×					1099:1099	×	1099:1099	×	1099:1099	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	4	4	contain	possessed	783:791	arg1	polysaccharides					724:738	P. ginseng polysaccharides	713:738	P. ginseng polysaccharides extracted by microwave-assisted extraction	713:781	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	4	contain	possessed	783:791	arg2	yield					813:817	a higher extraction yield	793:817	a higher extraction yield than hot water extraction (WPPG)	793:850	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	7	5	theme	uronic	1289:1294	arg1	content					1301:1307	uronic acid content	1289:1307	uronic acid content	1289:1307	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	4	6	theme	%	911:911	arg1	yields					895:900	the actual yields	884:900	the actual yields	884:900	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	6	theme	%	911:911	arg1	%					919:919	41.6% ± 0.09%	907:919	41.6% ± 0.09%	907:919	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	1	7	theme	Panax	173:177	arg1	ginseng					179:185	Panax ginseng	173:185	Panax ginseng	173:185	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	0	8	theme	Panax	111:115	arg1	ginseng					117:123	Panax ginseng	111:123	Panax ginseng	111:123	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	1	9	theme	P.	349:350	arg1	polysaccharides					360:374	P. ginseng polysaccharides	349:374	P. ginseng polysaccharides	349:374	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	10	10	theme	antibacterial	1713:1725	arg1	agent					1727:1731	an antioxidant and antibacterial agent	1694:1731	an antioxidant and antibacterial agent	1694:1731	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	10	10	theme	antibacterial	1713:1725	arg1	MPPG					1669:1672	MPPG	1669:1672	MPPG	1669:1672	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	4	11	theme	water	828:832	arg1	WPPG					846:849	WPPG	846:849	WPPG	846:849	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	11	theme	water	828:832	arg1	extraction					834:843	hot water extraction	824:843	hot water extraction (WPPG)	824:850	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	10	12	theme	antioxidant	1697:1707	arg1	agent					1727:1731	an antioxidant and antibacterial agent	1694:1731	an antioxidant and antibacterial agent	1694:1731	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	10	12	theme	antioxidant	1697:1707	arg1	MPPG					1669:1672	MPPG	1669:1672	MPPG	1669:1672	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	2	13	theme	microwave-assisted	525:542	arg1	composition					582:592	their chemical composition	567:592	their chemical composition	567:592	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	1	14	theme	ginseng	352:358	arg1	polysaccharides					360:374	P. ginseng polysaccharides	349:374	P. ginseng polysaccharides	349:374	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	4	15	theme	hot	824:826	arg1	WPPG					846:849	WPPG	846:849	WPPG	846:849	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	15	theme	hot	824:826	arg1	extraction					834:843	hot water extraction	824:843	hot water extraction (WPPG)	824:850	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	10	16	theme	effective	1606:1614	arg1	extraction					1589:1598	microwave-assisted extraction	1570:1598	microwave-assisted extraction	1570:1598	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	10	16	theme	effective	1606:1614	arg1	method					1616:1621	an effective method	1603:1621	an effective method for obtaining P. ginseng polysaccharides	1603:1662	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	2	17	dep	composition	582:592	arg1	compare					559:565	compare	559:565	compare	559:565	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	1	18	theme	polysaccharides	360:374	arg1	activity					337:344	the bactericidal activity	320:344	the bactericidal activity of P. ginseng polysaccharides	320:374	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	19	from	substance	160:168	arg1	ginseng					179:185	Panax ginseng	173:185	Panax ginseng	173:185	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	6	20	theme	2.07	1094:1097	arg1	×					1099:1099	×	1099:1099	×	1099:1099	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	8	21	theme	Mw	1442:1443	arg1	content					1470:1476	its highly Mw and abundant uronic acid content	1431:1476	its highly Mw and abundant uronic acid content	1431:1476	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	4	22	theme	actual	888:893	arg1	yields					895:900	the actual yields	884:900	the actual yields	884:900	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	22	theme	actual	888:893	arg1	%					919:919	41.6% ± 0.09%	907:919	41.6% ± 0.09%	907:919	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	5	23	theme	polysaccharides	1000:1014	arg1	characterization					980:995	the preliminary characterization	964:995	the preliminary characterization of polysaccharides	964:1014	Moreover, the preliminary characterization of polysaccharides was identified after purification.
31018583	4	24	theme	extraction	802:811	arg1	yield					813:817	a higher extraction yield	793:817	a higher extraction yield than hot water extraction (WPPG)	793:850	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	25	theme	P.	713:714	arg1	polysaccharides					724:738	P. ginseng polysaccharides	713:738	P. ginseng polysaccharides extracted by microwave-assisted extraction	713:781	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	0	26	theme	Microwave-Assisted	0:17	arg1	Extraction					19:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	8	27	theme	abundant	1449:1456	arg1	content					1470:1476	its highly Mw and abundant uronic acid content	1431:1476	its highly Mw and abundant uronic acid content	1431:1476	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	7	28	contain	had	1272:1274	arg1	MPPG					1267:1270	MPPG	1267:1270	MPPG	1267:1270	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	7	28	contain	had	1272:1274	arg2	content					1301:1307	uronic acid content	1289:1307	uronic acid content	1289:1307	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	7	28	contain	had	1272:1274	arg2	Mw					1285:1286	a higher Mw	1276:1286	a higher Mw	1276:1286	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	7	28	contain	had	1272:1274	arg2	content					1318:1324	Glu content	1314:1324	Glu content	1314:1324	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	4	29	theme	higher	795:800	arg1	yield					813:817	a higher extraction yield	793:817	a higher extraction yield than hot water extraction (WPPG)	793:850	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	6	30	theme	IR	1235:1236	arg1	spectra					1238:1244	IR spectra	1235:1244	IR spectra	1235:1244	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	7	31	theme	higher	1278:1283	arg1	Mw					1285:1286	a higher Mw	1276:1286	a higher Mw	1276:1286	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	6	32	with	WPPG	1055:1058	arg1	weight					1079:1084	the molecular weight	1065:1084	the molecular weight (Mw) of 2.07 × 105 Da	1065:1106	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	6	32	with	WPPG	1055:1058	arg1	Mw					1087:1088	Mw	1087:1088	Mw	1087:1088	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	7	33	theme	Glu	1314:1316	arg1	content					1318:1324	Glu content	1314:1324	Glu content	1314:1324	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	1	34	theme	microwave-assisted	197:214	arg1	extraction					216:225	microwave-assisted extraction	197:225	microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG)	197:275	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	8	35	theme	acid	1465:1468	arg1	content					1470:1476	its highly Mw and abundant uronic acid content	1431:1476	its highly Mw and abundant uronic acid content	1431:1476	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	2	36	theme	chemical	573:580	arg1	composition					582:592	their chemical composition	567:592	their chemical composition	567:592	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	10	37	theme	P.	1637:1638	arg1	polysaccharides					1648:1662	P. ginseng polysaccharides	1637:1662	P. ginseng polysaccharides	1637:1662	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	8	38	theme	antioxidant	1349:1359	arg1	activity					1361:1368	the antioxidant activity	1345:1368	the antioxidant activity of MPPG	1345:1376	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	8	38	theme	antioxidant	1349:1359	arg1	higher					1382:1387	higher	1382:1387	higher	1382:1387	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	0	39	theme	Partial	45:51	arg1	Characterization					53:68	Partial Characterization	45:68	Partial Characterization	45:68	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	8	40	theme	uronic	1458:1463	arg1	content					1470:1476	its highly Mw and abundant uronic acid content	1431:1476	its highly Mw and abundant uronic acid content	1431:1476	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	6	41	theme	typical	1173:1179	arg1	characteristics					1181:1195	the typical characteristics	1169:1195	the typical characteristics of polysaccharides	1169:1214	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	6	42	theme	molecular	1069:1077	arg1	weight					1079:1084	the molecular weight	1065:1084	the molecular weight (Mw) of 2.07 × 105 Da	1065:1106	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	6	42	theme	molecular	1069:1077	arg1	Mw					1087:1088	Mw	1087:1088	Mw	1087:1088	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	2	43	theme	polysaccharides	463:477	arg1	extraction					438:447	the extraction	434:447	the extraction of P. ginseng polysaccharides	434:477	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	9	44	theme	bactericidal	1516:1527	arg1	activity					1529:1536	high bactericidal activity	1511:1536	high bactericidal activity	1511:1536	Besides, both of them exhibited high bactericidal activity.
31018583	3	45	theme	antibacterial	628:640	arg1	activities					658:667	their antibacterial and antioxidant activities	622:667	their antibacterial and antioxidant activities	622:667	In addition, their antibacterial and antioxidant activities were also determined.
31018583	10	46	theme	microwave-assisted	1570:1587	arg1	extraction					1589:1598	microwave-assisted extraction	1570:1598	microwave-assisted extraction	1570:1598	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	10	46	theme	microwave-assisted	1570:1587	arg1	method					1616:1621	an effective method	1603:1621	an effective method for obtaining P. ginseng polysaccharides	1603:1662	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	2	47	theme	ginseng	455:461	arg1	polysaccharides					463:477	P. ginseng polysaccharides	452:477	P. ginseng polysaccharides	452:477	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	2	48	theme	extraction-and	544:557	arg1	composition					582:592	their chemical composition	567:592	their chemical composition	567:592	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	8	49	theme	MPPG	1373:1376	arg1	activity					1361:1368	the antioxidant activity	1345:1368	the antioxidant activity of MPPG	1345:1376	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	8	49	theme	MPPG	1373:1376	arg1	higher					1382:1387	higher	1382:1387	higher	1382:1387	More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content.
31018583	7	50	theme	acid	1296:1299	arg1	content					1301:1307	uronic acid content	1289:1307	uronic acid content	1289:1307	Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content.
31018583	2	51	theme	P.	452:453	arg1	polysaccharides					463:477	P. ginseng polysaccharides	452:477	P. ginseng polysaccharides	452:477	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	6	52	theme	polysaccharides	1200:1214	arg1	characteristics					1181:1195	the typical characteristics	1169:1195	the typical characteristics of polysaccharides	1169:1214	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	4	53	theme	microwave-assisted	753:770	arg1	extraction					772:781	microwave-assisted extraction	753:781	microwave-assisted extraction	753:781	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	4	54	theme	±	931:931	arg1	%					937:937	28.5% ± 1.62%	925:937	28.5% ± 1.62%	925:937	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	0	55	from	ginseng	117:123	arg1	Purification					31:42	Purification	31:42	Purification	31:42	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	55	from	ginseng	117:123	arg1	Characterization					53:68	Partial Characterization	45:68	Partial Characterization	45:68	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	55	from	ginseng	117:123	arg1	Extraction					19:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction	0:28	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	0	55	from	ginseng	117:123	arg1	Bioactivity					75:85	Bioactivity	75:85	Bioactivity	75:85	Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.
31018583	9	56	theme	high	1511:1514	arg1	activity					1529:1536	high bactericidal activity	1511:1536	high bactericidal activity	1511:1536	Besides, both of them exhibited high bactericidal activity.
31018583	10	57	theme	ginseng	1640:1646	arg1	polysaccharides					1648:1662	P. ginseng polysaccharides	1637:1662	P. ginseng polysaccharides	1637:1662	These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.
31018583	4	58	theme	%	929:929	arg1	%					937:937	28.5% ± 1.62%	925:937	28.5% ± 1.62%	925:937	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	5	59	theme	preliminary	968:978	arg1	characterization					980:995	the preliminary characterization	964:995	the preliminary characterization of polysaccharides	964:1014	Moreover, the preliminary characterization of polysaccharides was identified after purification.
31018583	1	60	theme	main	148:151	arg1	substance					160:168	a main active substance	146:168	a main active substance in Panax ginseng	146:185	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	60	theme	main	148:151	arg1	Polysaccharides					126:140	Polysaccharides	126:140	Polysaccharides	126:140	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	61	theme	P.	243:244	arg1	MPPG					271:274	MPPG	271:274	MPPG	271:274	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	61	theme	P.	243:244	arg1	polysaccharides					254:268	P. ginseng polysaccharides	243:268	P. ginseng polysaccharides (MPPG)	243:275	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	2	62	theme	water	504:508	arg1	extraction					510:519	two methods-hot water extraction	488:519	two methods-hot water extraction	488:519	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	6	63	theme	Da	1105:1106	arg1	weight					1079:1084	the molecular weight	1065:1084	the molecular weight (Mw) of 2.07 × 105 Da	1065:1106	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	6	63	theme	Da	1105:1106	arg1	Mw					1087:1088	Mw	1087:1088	Mw	1087:1088	The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra.
31018583	4	64	theme	optimized	858:866	arg1	conditions					868:877	optimized conditions	858:877	optimized conditions	858:877	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31018583	1	65	theme	active	153:158	arg1	substance					160:168	a main active substance	146:168	a main active substance in Panax ginseng	146:185	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	65	theme	active	153:158	arg1	Polysaccharides					126:140	Polysaccharides	126:140	Polysaccharides	126:140	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	66	theme	ginseng	246:252	arg1	MPPG					271:274	MPPG	271:274	MPPG	271:274	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	1	66	theme	ginseng	246:252	arg1	polysaccharides					254:268	P. ginseng polysaccharides	243:268	P. ginseng polysaccharides (MPPG)	243:275	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	2	67	theme	methods-hot	492:502	arg1	extraction					510:519	two methods-hot water extraction	488:519	two methods-hot water extraction	488:519	Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods-hot water extraction and microwave-assisted extraction-and compare their chemical composition and structure.
31018583	1	68	theme	bactericidal	324:335	arg1	activity					337:344	the bactericidal activity	320:344	the bactericidal activity of P. ginseng polysaccharides	320:374	Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low.
31018583	3	69	theme	antioxidant	646:656	arg1	activities					658:667	their antibacterial and antioxidant activities	622:667	their antibacterial and antioxidant activities	622:667	In addition, their antibacterial and antioxidant activities were also determined.
31018583	4	70	theme	ginseng	716:722	arg1	polysaccharides					724:738	P. ginseng polysaccharides	713:738	P. ginseng polysaccharides extracted by microwave-assisted extraction	713:781	The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively.
31805322	4	0	theme	linear	688:693	arg1	backbone					695:702	a linear backbone	686:702	a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose	686:785	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	2	1	theme	glucose	395:401	arg1	arabinose					441:449	arabinose	441:449	arabinose	441:449	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	galactose					456:464	galactose	456:464	galactose	456:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	amount					422:427	trace amount	416:427	trace amount of mannose, arabinose, and galactose	416:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	mannose					432:438	mannose	432:438	mannose	432:438	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	presence					371:378	the presence	367:378	the presence of predominant glucose (98.03%)	367:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	1	theme	glucose	395:401	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	1	2	theme	ethanol	259:265	arg1	extraction					247:256	hot water extraction	237:256	hot water extraction	237:256	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	1	2	theme	ethanol	259:265	arg1	precipitation					267:279	ethanol precipitation	259:279	ethanol precipitation	259:279	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	2	3	theme	predominant	383:393	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	3	theme	predominant	383:393	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	4	4	contain	has	682:684	arg1	GPN					678:680	GPN	678:680	GPN	678:680	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	4	4	contain	has	682:684	arg2	backbone					695:702	a linear backbone	686:702	a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose	686:785	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	3	5	theme	1,4-linked	555:564	arg1	Glcp					566:569	1,4-linked Glcp	555:569	1,4-linked Glcp	555:569	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	3	6	theme	T-linked	572:579	arg1	Glcp					581:584	T-linked Glcp	572:584	T-linked Glcp	572:584	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	6	7	theme	structure	971:979	arg1	existence					945:953	the existence	941:953	the existence of triple helix structure	941:979	Congo red assay confirmed the existence of triple helix structure.
31805322	4	8	theme	1,6-linked	731:740	arg1	α-D-Glcp					742:749	1,6-linked α-D-Glcp	731:749	1,6-linked α-D-Glcp	731:749	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	4	9	link	1,4-linked	707:716	arg1	α-D-Glcp					718:725	1,4-linked α-D-Glcp	707:725	1,4-linked α-D-Glcp	707:725	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	3	10	from	bonds	532:536	arg1	GPN					541:543	GPN	541:543	GPN	541:543	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	3	11	theme	main	516:519	arg1	bonds					532:536	the main glycosidic bonds	512:536	the main glycosidic bonds in GPN	512:543	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	6	12	theme	helix	965:969	arg1	structure					971:979	triple helix structure	958:979	triple helix structure	958:979	Congo red assay confirmed the existence of triple helix structure.
31805322	3	13	link	1,4-linked	555:564	arg1	Glcp					566:569	1,4-linked Glcp	555:569	1,4-linked Glcp	555:569	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	8	14	theme	favorable	1147:1155	arg1	stability					1165:1173	favorable thermal stability	1147:1173	favorable thermal stability	1147:1173	GPN also displayed favorable thermal stability.
31805322	3	15	theme	GC-MS	483:487	arg1	analysis					489:496	Methylation and GC-MS analysis	467:496	analysis	489:496	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	5	16	theme	residue	906:912	arg1	C-3					897:899	the C-3	893:899	the C-3 of D residue	893:912	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	1	17	theme	mass	181:184	arg1	38.7 kDa					186:193	molecular mass 38.7 kDa	171:193	molecular mass 38.7 kDa	171:193	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	5	18	link	1-linked	872:879	arg1	β-D-Glcp					881:888	terminal 1-linked β-D-Glcp	863:888	terminal 1-linked β-D-Glcp	863:888	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	8	19	theme	thermal	1157:1163	arg1	stability					1165:1173	favorable thermal stability	1147:1173	favorable thermal stability	1147:1173	GPN also displayed favorable thermal stability.
31805322	4	20	theme	1D/2D	654:658	arg1	spectroscopy					664:675	1D/2D NMR spectroscopy	654:675	1D/2D NMR spectroscopy	654:675	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	2	21	theme	galactose	456:464	arg1	arabinose					441:449	arabinose	441:449	arabinose	441:449	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	galactose					456:464	galactose	456:464	galactose	456:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	amount					422:427	trace amount	416:427	trace amount of mannose, arabinose, and galactose	416:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	mannose					432:438	mannose	432:438	mannose	432:438	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	presence					371:378	the presence	367:378	the presence of predominant glucose (98.03%)	367:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	21	theme	galactose	456:464	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	9	22	theme	G.glabra	1186:1193	arg1	polysaccharide					1195:1208	G.glabra polysaccharide	1186:1208	G.glabra polysaccharide	1186:1208	Moreover, G.glabra polysaccharide showed good antioxidant activity.
31805322	4	23	link	1,6-linked	731:740	arg1	α-D-Glcp					742:749	1,6-linked α-D-Glcp	731:749	1,6-linked α-D-Glcp	731:749	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	5	24	theme	terminal	863:870	arg1	β-D-Glcp					881:888	terminal 1-linked β-D-Glcp	863:888	terminal 1-linked β-D-Glcp	863:888	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	0	25	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra.
31805322	6	26	theme	triple	958:963	arg1	structure					971:979	triple helix structure	958:979	triple helix structure	958:979	Congo red assay confirmed the existence of triple helix structure.
31805322	1	27	with	polysaccharide	139:152	arg1	38.7 kDa					186:193	molecular mass 38.7 kDa	171:193	molecular mass 38.7 kDa	171:193	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	4	28	theme	NMR	660:662	arg1	spectroscopy					664:675	1D/2D NMR spectroscopy	654:675	1D/2D NMR spectroscopy	654:675	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	2	29	theme	composition	336:346	arg1	analysis					348:355	Monosaccharide composition analysis	321:355	Monosaccharide composition analysis	321:355	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	7	30	contain	had	1035:1037	arg2	fibrous					1049:1055	fibrous	1049:1055	fibrous	1049:1055	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	7	30	contain	had	1035:1037	arg2	filaments					1058:1066	filaments	1058:1066	filaments	1058:1066	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	7	30	contain	had	1035:1037	arg1	GPN					1031:1033	the GPN	1027:1033	the GPN	1027:1033	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	5	31	theme	1-linked	872:879	arg1	β-D-Glcp					881:888	terminal 1-linked β-D-Glcp	863:888	terminal 1-linked β-D-Glcp	863:888	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	0	32	theme	antioxidant	32:42	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra.
31805322	2	33	theme	Monosaccharide	321:334	arg1	analysis					348:355	Monosaccharide composition analysis	321:355	Monosaccharide composition analysis	321:355	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	arabinose					441:449	arabinose	441:449	arabinose	441:449	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	galactose					456:464	galactose	456:464	galactose	456:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	amount					422:427	trace amount	416:427	trace amount of mannose, arabinose, and galactose	416:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	mannose					432:438	mannose	432:438	mannose	432:438	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	presence					371:378	the presence	367:378	the presence of predominant glucose (98.03%)	367:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	34	theme	arabinose	441:449	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	1	35	theme	column	298:303	arg1	chromatography					305:318	column chromatography	298:318	column chromatography	298:318	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	9	36	theme	good	1217:1220	arg1	activity					1234:1241	good antioxidant activity	1217:1241	good antioxidant activity	1217:1241	Moreover, G.glabra polysaccharide showed good antioxidant activity.
31805322	1	37	theme	molecular	171:179	arg1	38.7 kDa					186:193	molecular mass 38.7 kDa	171:193	molecular mass 38.7 kDa	171:193	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	2	38	theme	mannose	432:438	arg1	arabinose					441:449	arabinose	441:449	arabinose	441:449	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	galactose					456:464	galactose	456:464	galactose	456:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	amount					422:427	trace amount	416:427	trace amount of mannose, arabinose, and galactose	416:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	mannose					432:438	mannose	432:438	mannose	432:438	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	presence					371:378	the presence	367:378	the presence of predominant glucose (98.03%)	367:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	38	theme	mannose	432:438	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	3	39	link	1,4,6-linked	587:598	arg1	Glcp					600:603	1,4,6-linked Glcp	587:603	1,4,6-linked Glcp	587:603	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	9	40	theme	antioxidant	1222:1232	arg1	activity					1234:1241	good antioxidant activity	1217:1241	good antioxidant activity	1217:1241	Moreover, G.glabra polysaccharide showed good antioxidant activity.
31805322	5	41	theme	main	840:843	arg1	α-D-Glcp					850:857	main side α-D-Glcp	840:857	main side α-D-Glcp	840:857	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	2	42	theme	trace	416:420	arg1	arabinose					441:449	arabinose	441:449	arabinose	441:449	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	42	theme	trace	416:420	arg1	glucose					395:401	predominant glucose	383:401	predominant glucose (98.03%)	383:410	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	42	theme	trace	416:420	arg1	galactose					456:464	galactose	456:464	galactose	456:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	42	theme	trace	416:420	arg1	amount					422:427	trace amount	416:427	trace amount of mannose, arabinose, and galactose	416:464	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	42	theme	trace	416:420	arg1	mannose					432:438	mannose	432:438	mannose	432:438	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	2	42	theme	trace	416:420	arg1	%					409:409	98.03%	404:409	98.03%	404:409	Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose.
31805322	1	43	attach	isolated	199:206	arg1	glabra					225:230	glabra	225:230	glabra	225:230	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	1	43	attach	isolated	199:206	arg2	polysaccharide					139:152	A water-soluble polysaccharide	123:152	A water-soluble polysaccharide	123:152	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	3	44	theme	Methylation	467:477	arg1	analysis					489:496	Methylation and GC-MS analysis	467:496	analysis	489:496	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	3	45	link	T-linked	572:579	arg1	Glcp					581:584	T-linked Glcp	572:584	T-linked Glcp	572:584	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	3	46	theme	1,4,6-linked	587:598	arg1	Glcp					600:603	1,4,6-linked Glcp	587:603	1,4,6-linked Glcp	587:603	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	7	47	theme	XRD	1000:1002	arg1	analysis					1004:1011	SEM and XRD analysis	992:1011	SEM and XRD analysis	992:1011	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	1	48	theme	water-soluble	125:137	arg1	polysaccharide					139:152	A water-soluble polysaccharide	123:152	A water-soluble polysaccharide	123:152	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	5	49	theme	side	792:795	arg1	chain					797:801	The side chain	788:801	The side chain	788:801	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	6	50	theme	red	921:923	arg1	assay					925:929	Congo red assay	915:929	Congo red assay	915:929	Congo red assay confirmed the existence of triple helix structure.
31805322	3	51	theme	glycosidic	521:530	arg1	bonds					532:536	the main glycosidic bonds	512:536	the main glycosidic bonds in GPN	512:543	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	5	52	theme	D	904:904	arg1	residue					906:912	D residue	904:912	D residue	904:912	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	6	53	theme	Congo	915:919	arg1	assay					925:929	Congo red assay	915:929	Congo red assay	915:929	Congo red assay confirmed the existence of triple helix structure.
31805322	7	54	theme	SEM	992:994	arg1	analysis					1004:1011	SEM and XRD analysis	992:1011	SEM and XRD analysis	992:1011	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	4	55	theme	α-D-Glcp	718:725	arg1	backbone					695:702	a linear backbone	686:702	a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose	686:785	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	1	56	theme	hot	237:239	arg1	extraction					247:256	hot water extraction	237:256	hot water extraction	237:256	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	1	56	theme	hot	237:239	arg1	precipitation					267:279	ethanol precipitation	259:279	ethanol precipitation	259:279	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	4	57	theme	glucose	779:785	arg1	C-4					772:774	C-4	772:774	C-4 of glucose	772:785	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	4	58	theme	α-D-Glcp	742:749	arg1	backbone					695:702	a linear backbone	686:702	a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose	686:785	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	4	59	theme	1,4-linked	707:716	arg1	α-D-Glcp					718:725	1,4-linked α-D-Glcp	707:725	1,4-linked α-D-Glcp	707:725	Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose.
31805322	1	60	theme	water	241:245	arg1	extraction					247:256	hot water extraction	237:256	hot water extraction	237:256	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	1	60	theme	water	241:245	arg1	precipitation					267:279	ethanol precipitation	259:279	ethanol precipitation	259:279	A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography.
31805322	0	61	theme	polysaccharide	74:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra.
31805322	0	61	theme	polysaccharide	74:87	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra.
31805322	5	62	theme	side	845:848	arg1	α-D-Glcp					850:857	main side α-D-Glcp	840:857	main side α-D-Glcp	840:857	The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue.
31805322	3	63	theme	1,6-linked	610:619	arg1	Glcp					621:624	1,6-linked Glcp	610:624	1,6-linked Glcp	610:624	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
31805322	0	64	theme	soluble	66:72	arg1	polysaccharide					74:87	a water soluble polysaccharide	58:87	a water soluble polysaccharide	58:87	Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra.
31805322	7	65	dep	crystalline	1091:1101	arg1	structure					1117:1125	structure	1117:1125	structure	1117:1125	Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure.
31805322	3	66	link	1,6-linked	610:619	arg1	Glcp					621:624	1,6-linked Glcp	610:624	1,6-linked Glcp	610:624	Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp.
30192956	0	0	theme	chemical	64:71	arg1	composition					73:83	diverse chemical composition	56:83	diverse chemical composition	56:83	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	4	1	theme	perennial	844:852	arg1	ryegrass					854:861	perennial ryegrass	844:861	perennial ryegrass	844:861	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	9	2	theme	model	1532:1536	arg1	utility					1517:1523	the utility	1513:1523	the utility of the model as a predictive tool	1513:1557	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	2	3	dep	stoichiometry	520:532	arg1	settings					535:542	settings	535:542	settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios	535:611	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	1	4	theme	perennial	241:249	arg1	chicory					275:281	chicory	275:281	chicory	275:281	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	4	theme	perennial	241:249	arg1	ryegrass					251:258	sheep fed perennial ryegrass	231:258	sheep fed perennial ryegrass	231:258	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	4	theme	perennial	241:249	arg1	clover					267:272	white clover	261:272	white clover	261:272	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	0	5	theme	diverse	56:62	arg1	composition					73:83	diverse chemical composition	56:83	diverse chemical composition	56:83	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	8	6	theme	fodder	1406:1411	arg1	crops					1413:1417	annual fodder crops	1399:1417	annual fodder crops	1399:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	6	7	theme	acetogenic	1116:1125	arg1	stoichiometry					1127:1139	a predominantly acetogenic stoichiometry	1100:1139	a predominantly acetogenic stoichiometry	1100:1139	Except for forage rape, robust predictions were obtained for all forages using fast degradation kinetics and a predominantly acetogenic stoichiometry.
30192956	10	8	theme	digestion	1602:1610	arg1	rates					1612:1616	digestion rates	1602:1616	digestion rates	1602:1616	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	8	9	theme	annual	1399:1404	arg1	crops					1413:1417	annual fodder crops	1399:1417	annual fodder crops	1399:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	3	10	theme	acetate	733:739	arg1	ratio					755:759	acetate to propionate ratio	733:759	acetate to propionate ratio (A:P)	733:765	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	1	11	theme	bulb	315:318	arg1	varieties					320:328	bulb varieties	315:328	bulb varieties	315:328	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	3	12	dep	ratio	755:759	arg1	to					741:742	to	741:742	to	741:742	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	12	dep	ratio	755:759	arg1	A					762:762	A	762:762	A:P	762:764	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	9	13	theme	stoichiometries	1476:1490	arg1	understanding					1437:1449	a clear understanding	1429:1449	a clear understanding of degradation rates and stoichiometries	1429:1490	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	0	14	theme	composition	73:83	arg1	forages					45:51	fresh forages	39:51	fresh forages of diverse chemical composition	39:83	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	9	15	theme	predictive	1543:1552	arg1	tool					1554:1557	a predictive tool	1541:1557	a predictive tool	1541:1557	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	2	16	theme	fatty	595:599	arg1	acid					601:604	glucogenic short-chain fatty acid	572:604	glucogenic short-chain fatty acid	572:604	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	9	17	theme	clear	1431:1435	arg1	understanding					1437:1449	a clear understanding	1429:1449	a clear understanding of degradation rates and stoichiometries	1429:1490	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	1	18	theme	white	261:265	arg1	ryegrass					251:258	sheep fed perennial ryegrass	231:258	sheep fed perennial ryegrass	231:258	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	18	theme	white	261:265	arg1	clover					267:272	white clover	261:272	white clover	261:272	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	7	19	dep	rape	1171:1174	arg1	forage					1164:1169	forage	1164:1169	forage	1164:1169	Model predictions for forage rape were enhanced using slow degradation kinetics and a predominantly propionic stoichiometry.
30192956	1	20	dep	turnip	297:302	arg1	leafy					305:309	leafy	305:309	leafy	305:309	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	20	dep	turnip	297:302	arg1	varieties					320:328	bulb varieties	315:328	bulb varieties	315:328	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	2	21	theme	glucogenic	572:581	arg1	acid					601:604	glucogenic short-chain fatty acid	572:604	glucogenic short-chain fatty acid	572:604	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	2	22	theme	short-chain	583:593	arg1	acid					601:604	glucogenic short-chain fatty acid	572:604	glucogenic short-chain fatty acid	572:604	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	7	23	theme	degradation	1201:1211	arg1	kinetics					1213:1220	slow degradation kinetics	1196:1220	slow degradation kinetics	1196:1220	Model predictions for forage rape were enhanced using slow degradation kinetics and a predominantly propionic stoichiometry.
30192956	2	24	theme	degradation	410:420	arg1	rate					422:425	substrate degradation rate	400:425	substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion)	400:498	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	3	25	dep	A	762:762	arg1	P					764:764	P	764:764	A:P	762:764	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	6	26	theme	robust	1015:1020	arg1	predictions					1022:1032	robust predictions	1015:1032	robust predictions	1015:1032	Except for forage rape, robust predictions were obtained for all forages using fast degradation kinetics and a predominantly acetogenic stoichiometry.
30192956	1	27	theme	VFA	155:157	arg1	predictions					159:169	VFA predictions	155:169	VFA predictions	155:169	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	2	28	theme	substrate	400:408	arg1	rate					422:425	substrate degradation rate	400:425	substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion)	400:498	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	3	29	theme	methane	665:671	arg1	g/d					691:693	pCH4: g/d	685:693	pCH4: g/d	685:693	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	29	theme	methane	665:671	arg1	production					673:682	methane production	665:682	methane production (pCH4: g/d)	665:694	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	2	30	theme	nonglucogenic	555:567	arg1	ratios					606:611	nonglucogenic to glucogenic short-chain fatty acid ratios	555:611	nonglucogenic to glucogenic short-chain fatty acid ratios	555:611	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	9	31	theme	degradation	1454:1464	arg1	rates					1466:1470	degradation rates	1454:1470	degradation rates	1454:1470	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	9	32	theme	rates	1466:1470	arg1	understanding					1437:1449	a clear understanding	1429:1449	a clear understanding of degradation rates and stoichiometries	1429:1490	However, a clear understanding of degradation rates and stoichiometries is needed to enhance the utility of the model as a predictive tool.
30192956	6	33	dep	rape	1009:1012	arg1	forage					1002:1007	forage	1002:1007	forage	1002:1007	Except for forage rape, robust predictions were obtained for all forages using fast degradation kinetics and a predominantly acetogenic stoichiometry.
30192956	0	34	theme	sheep	16:20	arg1	model					28:32	a sheep rumen model	14:32	a sheep rumen model	14:32	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	2	35	theme	digestion	489:497	arg1	rate					453:456	the rate	449:456	the rate of cellulose and hemicellulose digestion	449:497	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	10	36	theme	individual	1668:1677	arg1	species					1686:1692	individual forage species	1668:1692	individual forage species	1668:1692	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	3	37	theme	pCH4	685:688	arg1	g/d					691:693	pCH4: g/d	685:693	pCH4: g/d	685:693	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	37	theme	pCH4	685:688	arg1	production					673:682	methane production	665:682	methane production (pCH4: g/d)	665:694	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	2	38	theme	hemicellulose	475:487	arg1	digestion					489:497	hemicellulose digestion	475:497	hemicellulose digestion	475:497	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	1	39	theme	sheep	90:94	arg1	MollyRum14					111:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	10	40	theme	stoichiometries	1622:1636	arg1	adjustment					1588:1597	continuous adjustment	1577:1597	continuous adjustment of digestion rates and stoichiometries	1577:1636	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	0	41	theme	model	28:32	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.	0:84	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	7	42	theme	slow	1196:1199	arg1	kinetics					1213:1220	slow degradation kinetics	1196:1220	slow degradation kinetics	1196:1220	Model predictions for forage rape were enhanced using slow degradation kinetics and a predominantly propionic stoichiometry.
30192956	1	43	theme	respiration-chamber	189:207	arg1	trials					209:214	respiration-chamber trials	189:214	respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory,	189:282	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	0	44	theme	rumen	22:26	arg1	model					28:32	a sheep rumen model	14:32	a sheep rumen model	14:32	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	2	45	theme	cellulose	461:469	arg1	rate					453:456	the rate	449:456	the rate of cellulose and hemicellulose digestion	449:497	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	3	46	theme	yCH4	712:715	arg1	DMI					723:725	yCH4: g/kg DMI	712:725	yCH4: g/kg DMI	712:725	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	46	theme	yCH4	712:715	arg1	yield					705:709	methane yield	697:709	methane yield (yCH4: g/kg DMI)	697:726	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	0	47	theme	fresh	39:43	arg1	forages					45:51	fresh forages	39:51	fresh forages of diverse chemical composition	39:83	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	2	48	theme	fermentation	507:518	arg1	stoichiometry					520:532	fermentation stoichiometry	507:532	fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios)	507:612	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	6	49	theme	degradation	1075:1085	arg1	kinetics					1087:1094	fast degradation kinetics	1070:1094	fast degradation kinetics	1070:1094	Except for forage rape, robust predictions were obtained for all forages using fast degradation kinetics and a predominantly acetogenic stoichiometry.
30192956	1	50	from	trials	209:214	arg1	data					179:182	data	179:182	data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory,	179:282	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	51	theme	rumen	96:100	arg1	MollyRum14					111:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	4	52	theme	model	798:802	arg1	superior					831:838	superior	831:838	superior	831:838	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	4	52	theme	model	798:802	arg1	ability					783:789	The predictive ability	768:789	The predictive ability of the model for both pCH4 and yCH4	768:825	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	2	53	dep	rate	422:425	arg1	settings					428:435	settings	428:435	settings that affect the rate of cellulose and hemicellulose digestion	428:497	We assessed the model's response to substrate degradation rate (settings that affect the rate of cellulose and hemicellulose digestion) and to fermentation stoichiometry (settings that alter nonglucogenic to glucogenic short-chain fatty acid ratios).
30192956	3	54	theme	Model	615:619	arg1	predictions					621:631	Model predictions	615:631	Model predictions	615:631	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	6	55	theme	fast	1070:1073	arg1	kinetics					1087:1094	fast degradation kinetics	1070:1094	fast degradation kinetics	1070:1094	Except for forage rape, robust predictions were obtained for all forages using fast degradation kinetics and a predominantly acetogenic stoichiometry.
30192956	1	56	theme	submodel	102:109	arg1	MollyRum14					111:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14	86:120	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	3	57	theme	methane	697:703	arg1	DMI					723:725	yCH4: g/kg DMI	712:725	yCH4: g/kg DMI	712:725	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	57	theme	methane	697:703	arg1	yield					705:709	methane yield	697:709	methane yield (yCH4: g/kg DMI)	697:726	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	0	58	with	Evaluation	0:9	arg1	forages					45:51	fresh forages	39:51	fresh forages of diverse chemical composition	39:83	Evaluation of a sheep rumen model with fresh forages of diverse chemical composition.
30192956	10	59	theme	continuous	1577:1586	arg1	adjustment					1588:1597	continuous adjustment	1577:1597	continuous adjustment of digestion rates and stoichiometries	1577:1636	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	3	60	theme	g/kg	718:721	arg1	DMI					723:725	yCH4: g/kg DMI	712:725	yCH4: g/kg DMI	712:725	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	3	60	theme	g/kg	718:721	arg1	yield					705:709	methane yield	697:709	methane yield (yCH4: g/kg DMI)	697:726	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	4	61	theme	predictive	772:781	arg1	superior					831:838	superior	831:838	superior	831:838	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	4	61	theme	predictive	772:781	arg1	ability					783:789	The predictive ability	768:789	The predictive ability of the model for both pCH4 and yCH4	768:825	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	8	62	theme	fresh	1375:1379	arg1	forages					1381:1387	fresh forages	1375:1387	fresh forages including annual fodder crops	1375:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	8	62	theme	fresh	1375:1379	arg1	crops					1413:1417	annual fodder crops	1399:1417	annual fodder crops	1399:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	7	63	theme	propionic	1242:1250	arg1	stoichiometry					1252:1264	a predominantly propionic stoichiometry	1226:1264	a predominantly propionic stoichiometry	1226:1264	Model predictions for forage rape were enhanced using slow degradation kinetics and a predominantly propionic stoichiometry.
30192956	8	64	theme	forages	1381:1387	arg1	variety					1364:1370	a variety	1362:1370	a variety of fresh forages including annual fodder crops	1362:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	8	64	theme	forages	1381:1387	arg1	forages					1381:1387	fresh forages	1375:1387	fresh forages including annual fodder crops	1375:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	8	64	theme	forages	1381:1387	arg1	crops					1413:1417	annual fodder crops	1399:1417	annual fodder crops	1399:1417	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	7	65	theme	Model	1142:1146	arg1	predictions					1148:1158	Model predictions	1142:1158	Model predictions for forage rape	1142:1174	Model predictions for forage rape were enhanced using slow degradation kinetics and a predominantly propionic stoichiometry.
30192956	10	66	dep	species	1686:1692	arg1	forage					1679:1684	forage	1679:1684	forage	1679:1684	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	8	67	theme	methane	1329:1335	arg1	emissions					1337:1345	methane emissions	1329:1345	methane emissions	1329:1345	These results indicate that MollyRum14 is suitable to predict methane emissions from sheep fed a variety of fresh forages including annual fodder crops.
30192956	10	68	theme	rates	1612:1616	arg1	adjustment					1588:1597	continuous adjustment	1577:1597	continuous adjustment of digestion rates and stoichiometries	1577:1636	This would allow continuous adjustment of digestion rates and stoichiometries to be potentially tailored to individual forage species.
30192956	3	69	theme	propionate	744:753	arg1	ratio					755:759	acetate to propionate ratio	733:759	acetate to propionate ratio (A:P)	733:765	Model predictions were evaluated against data for methane production (pCH4: g/d), methane yield (yCH4: g/kg DMI), and acetate to propionate ratio (A:P).
30192956	1	70	theme	sheep	231:235	arg1	chicory					275:281	chicory	275:281	chicory	275:281	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	70	theme	sheep	231:235	arg1	ryegrass					251:258	sheep fed perennial ryegrass	231:258	sheep fed perennial ryegrass	231:258	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	70	theme	sheep	231:235	arg1	clover					267:272	white clover	261:272	white clover	261:272	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	4	71	theme	other	872:876	arg1	forages					878:884	other forages	872:884	other forages	872:884	The predictive ability of the model for both pCH4 and yCH4 was superior for perennial ryegrass than for other forages.
30192956	1	72	theme	fed	237:239	arg1	chicory					275:281	chicory	275:281	chicory	275:281	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	72	theme	fed	237:239	arg1	ryegrass					251:258	sheep fed perennial ryegrass	231:258	sheep fed perennial ryegrass	231:258	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30192956	1	72	theme	fed	237:239	arg1	clover					267:272	white clover	261:272	white clover	261:272	The sheep rumen submodel MollyRum14 was evaluated on its methane and VFA predictions against data from respiration-chamber trials conducted with sheep fed perennial ryegrass, white clover, chicory, forage rape, turnip (leafy and bulb varieties), swedes, kale, or forage radish.
30205128	11	0	theme	casting	1335:1341	arg1	technique					1343:1351	the consolidated solvent casting technique	1310:1351	the consolidated solvent casting technique	1310:1351	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	1	1	theme	orodispersible	163:176	arg1	ODF					185:187	ODF	185:187	ODF	185:187	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	1	1	theme	orodispersible	163:176	arg1	films					178:182	orodispersible films	163:182	maltodextrins orodispersible films (ODF)	149:188	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	7	2	theme	heating	866:872	arg1	temperature					874:884	heating temperature	866:884	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	8	3	theme	maximum	1017:1023	arg1	loading					1030:1036	The maximum drug loading	1013:1036	The maximum drug loading	1013:1036	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	8	3	theme	maximum	1017:1023	arg1	%					1050:1050	about 40%	1042:1050	about 40%	1042:1050	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	8	4	used	used	1074:1077	arg2	drug					1088:1091	model drug	1082:1091	model drug	1082:1091	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	8	4	used	used	1074:1077	arg2	paracetamol					1058:1068	paracetamol	1058:1068	paracetamol	1058:1068	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	7	5	theme	material	931:938	arg1	distance					945:952	needle-packaging material foil distance	914:952	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	4	6	theme	heated	529:534	arg1	chamber					497:503	the chamber	493:503	the chamber of the ram-extruder and heated	493:534	Then, the mixture is fed in the chamber of the ram-extruder and heated.
30205128	8	7	theme	model	1082:1086	arg1	drug					1088:1091	model drug	1082:1091	model drug	1082:1091	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	8	7	theme	model	1082:1086	arg1	paracetamol					1058:1068	paracetamol	1058:1068	paracetamol	1058:1068	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	7	8	dep	print	792:796	arg1	mixture					800:806	a mixture	798:806	print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio	792:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	5	9	theme	further	616:622	arg1	manipulations					624:636	further manipulations	616:636	further manipulations	616:636	ODF are individually printed on the packaging material foil and sealed without further manipulations.
30205128	7	10	theme	foil	940:943	arg1	distance					945:952	needle-packaging material foil distance	914:952	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	3	11	dep	glycerine	453:461	arg1	i.e.					448:451	i.e.	448:451	i.e.	448:451	First, maltodextrins, drug, and other excipients are mixed in a mortar and wetted with the plasticizer (i.e. glycerine).
30205128	10	12	theme	disintegration	1163:1176	arg1	time					1178:1181	disintegration time	1163:1181	disintegration time (<1 min)	1163:1190	specifications for disintegration time (<1 min).
30205128	10	12	theme	disintegration	1163:1176	arg1	<1 min					1184:1189	<1 min	1184:1189	<1 min	1184:1189	specifications for disintegration time (<1 min).
30205128	7	13	theme	w/w	846:848	arg1	ratio					850:854	a 80/20 w/w ratio	838:854	a 80/20 w/w ratio	838:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	14	theme	80/20	840:844	arg1	ratio					850:854	a 80/20 w/w ratio	838:854	a 80/20 w/w ratio	838:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	1	15	theme	preparation	134:144	arg1	feasibility					100:110	the feasibility	96:110	the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing	96:226	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	11	16	theme	consolidated	1314:1325	arg1	technique					1343:1351	the consolidated solvent casting technique	1310:1351	the consolidated solvent casting technique	1310:1351	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	5	17	theme	packaging	573:581	arg1	foil					592:595	the packaging material foil	569:595	the packaging material foil	569:595	ODF are individually printed on the packaging material foil and sealed without further manipulations.
30205128	7	18	theme	needle-packaging	914:929	arg1	distance					945:952	needle-packaging material foil distance	914:952	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	6	19	theme	process	679:685	arg1	variables					687:695	process variables	679:695	process variables	679:695	The critical formulation attributes and process variables were investigated to define the processability space.
30205128	11	20	theme	solvent	1327:1333	arg1	technique					1343:1351	the consolidated solvent casting technique	1310:1351	the consolidated solvent casting technique	1310:1351	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	5	21	theme	material	583:590	arg1	foil					592:595	the packaging material foil	569:595	the packaging material foil	569:595	ODF are individually printed on the packaging material foil and sealed without further manipulations.
30205128	0	22	theme	orodispersible	13:26	arg1	films					28:32	orodispersible films	13:32	orodispersible films	13:32	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	7	23	dep	resulted	856:863	arg1	temperature					874:884	heating temperature	866:884	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	24	from	print	792:796	arg1	ratio					850:854	a 80/20 w/w ratio	838:854	a 80/20 w/w ratio	838:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	25	theme	needle	894:899	arg1	gauge					901:905	needle gauge	894:905	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	11	26	theme	paracetamol	1220:1230	arg1	pattern					1209:1215	The dissolution pattern	1193:1215	The dissolution pattern of paracetamol	1193:1230	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	6	27	theme	formulation	652:662	arg1	attributes					664:673	The critical formulation attributes	639:673	The critical formulation attributes	639:673	The critical formulation attributes and process variables were investigated to define the processability space.
30205128	11	28	theme	dissolution	1197:1207	arg1	pattern					1209:1215	The dissolution pattern	1193:1215	The dissolution pattern of paracetamol	1193:1230	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	8	29	theme	drug	1025:1028	arg1	loading					1030:1036	The maximum drug loading	1013:1036	The maximum drug loading	1013:1036	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	8	29	theme	drug	1025:1028	arg1	%					1050:1050	about 40%	1042:1050	about 40%	1042:1050	The maximum drug loading was about 40%, when paracetamol was used as model drug.
30205128	9	30	theme	compounded	1098:1107	arg1	ODF					1109:1111	The compounded ODF	1094:1111	The compounded ODF	1094:1111	The compounded ODF complied with USP and Ph. Eur.
30205128	6	31	theme	critical	643:650	arg1	attributes					664:673	The critical formulation attributes	639:673	The critical formulation attributes	639:673	The critical formulation attributes and process variables were investigated to define the processability space.
30205128	1	32	theme	hot-melt	193:200	arg1	printing					219:226	hot-melt ram-extrusion 3D printing	193:226	hot-melt ram-extrusion 3D printing	193:226	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	7	33	theme	optimal	770:776	arg1	conditions					778:787	the optimal conditions	766:787	the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio	766:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	34	theme	maximum	963:969	arg1	rate					977:980	maximum print rate	963:980	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	1	35	dep	maltodextrins	149:161	arg1	ODF					185:187	ODF	185:187	ODF	185:187	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	1	35	dep	maltodextrins	149:161	arg1	films					178:182	orodispersible films	163:182	maltodextrins orodispersible films (ODF)	149:188	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	1	36	theme	ram-extrusion	202:214	arg1	printing					219:226	hot-melt ram-extrusion 3D printing	193:226	hot-melt ram-extrusion 3D printing	193:226	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	0	37	theme	melt	41:44	arg1	printing					63:70	hot melt ram extrusion 3D printing	37:70	hot melt ram extrusion 3D printing	37:70	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	4	38	theme	ram-extruder	512:523	arg1	chamber					497:503	the chamber	493:503	the chamber of the ram-extruder and heated	493:534	Then, the mixture is fed in the chamber of the ram-extruder and heated.
30205128	1	39	theme	3D	216:217	arg1	printing					219:226	hot-melt ram-extrusion 3D printing	193:226	hot-melt ram-extrusion 3D printing	193:226	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	0	40	theme	hot	37:39	arg1	printing					63:70	hot melt ram extrusion 3D printing	37:70	hot melt ram extrusion 3D printing	37:70	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	11	41	theme	similar	1278:1284	arg1	composition					1286:1296	a similar composition	1276:1296	a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology	1276:1409	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	7	42	dep	temperature	874:884	arg1	0.6 mm					955:960	0.6 mm	955:960	0.6 mm	955:960	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	50 mm/s					983:989	50 mm/s	983:989	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	85 °C					887:891	85 °C	887:891	85 °C	887:891	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	angle					1000:1004	filling angle	992:1004	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	rate					977:980	maximum print rate	963:980	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	gauge					901:905	needle gauge	894:905	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	120°					1007:1010	120°	1007:1010	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	distance					945:952	needle-packaging material foil distance	914:952	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	42	dep	temperature	874:884	arg1	18 G					908:911	18 G	908:911	18 G	908:911	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	0	43	theme	extrusion	50:58	arg1	printing					63:70	hot melt ram extrusion 3D printing	37:70	hot melt ram extrusion 3D printing	37:70	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	7	44	theme	maltodextrins/glycerine	811:833	arg1	mixture					800:806	a mixture	798:806	print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio	792:854	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	7	45	theme	filling	992:998	arg1	angle					1000:1004	filling angle	992:1004	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	0	46	theme	ram	46:48	arg1	printing					63:70	hot melt ram extrusion 3D printing	37:70	hot melt ram extrusion 3D printing	37:70	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	1	47	theme	maltodextrins	149:161	arg1	preparation					134:144	the extemporaneous preparation	115:144	the extemporaneous preparation of maltodextrins orodispersible films (ODF)	115:188	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	6	48	theme	processability	729:742	arg1	space					744:748	the processability space	725:748	the processability space	725:748	The critical formulation attributes and process variables were investigated to define the processability space.
30205128	11	49	theme	technology	1400:1409	arg1	suitability					1372:1382	the suitability	1368:1382	the suitability of the proposed technology	1368:1409	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	2	50	theme	technological	266:278	arg1	operations					280:289	three simple technological operations	253:289	three simple technological operations which can be also implemented in a pharmacy setting	253:341	This method consists of three simple technological operations which can be also implemented in a pharmacy setting.
30205128	7	51	theme	print	971:975	arg1	rate					977:980	maximum print rate	963:980	heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°	866:1010	In particular, the optimal conditions to print a mixture of maltodextrins/glycerine in a 80/20 w/w ratio resulted: heating temperature: 85 °C; needle gauge: 18 G; needle-packaging material foil distance: 0.6 mm; maximum print rate: 50 mm/s; filling angle: 120°.
30205128	1	52	theme	extemporaneous	119:132	arg1	preparation					134:144	the extemporaneous preparation	115:144	the extemporaneous preparation of maltodextrins orodispersible films (ODF)	115:188	This work demonstrates the feasibility of the extemporaneous preparation of maltodextrins orodispersible films (ODF) by hot-melt ram-extrusion 3D printing.
30205128	2	53	theme	simple	259:264	arg1	operations					280:289	three simple technological operations	253:289	three simple technological operations which can be also implemented in a pharmacy setting	253:341	This method consists of three simple technological operations which can be also implemented in a pharmacy setting.
30205128	2	54	theme	pharmacy	326:333	arg1	setting					335:341	a pharmacy setting	324:341	a pharmacy setting	324:341	This method consists of three simple technological operations which can be also implemented in a pharmacy setting.
30205128	11	55	theme	proposed	1391:1398	arg1	technology					1400:1409	the proposed technology	1387:1409	the proposed technology	1387:1409	The dissolution pattern of paracetamol overlapped with that obtained from ODF with a similar composition prepared by the consolidated solvent casting technique, demonstrating the suitability of the proposed technology.
30205128	0	56	theme	3D	60:61	arg1	printing					63:70	hot melt ram extrusion 3D printing	37:70	hot melt ram extrusion 3D printing	37:70	Personalized orodispersible films by hot melt ram extrusion 3D printing.
30205128	3	57	theme	other	376:380	arg1	excipients					382:391	other excipients	376:391	other excipients	376:391	First, maltodextrins, drug, and other excipients are mixed in a mortar and wetted with the plasticizer (i.e. glycerine).
29786840	7	0	theme	natural	1370:1376	arg1	dye					1378:1380	a natural dye	1368:1380	a natural dye for food and pharmaceutical products	1368:1417	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	7	0	theme	natural	1370:1376	arg1	application					1353:1363	possible application	1344:1363	possible application	1344:1363	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	4	1	theme	anthocyanins	775:786	arg1	retention					788:796	high anthocyanins retention	770:796	high anthocyanins retention (>87.5%)	770:805	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	1	theme	anthocyanins	775:786	arg1	%					804:804	>87.5%	799:804	>87.5%	799:804	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	2	theme	Glass	128:132	arg1	Temperature					145:155	Glass Transition Temperature	128:155	Glass Transition Temperature	128:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	10	3	theme	product	1774:1780	arg1	life					1762:1765	the shelf life	1752:1765	the shelf life of the product	1752:1780	Therefore, a milk-blackberry powder becomes an alternative, which may reduce postharvesting waste and increase the shelf life of the product without significantly altering its nutritional characteristics.
29786840	9	4	theme	Milk	1530:1533	arg1	components					1535:1544	Milk components	1530:1544	Milk components	1530:1544	Milk components could act as a wall material and emulsifying agent, protecting the flavonoids from blackberry.
29786840	9	4	theme	Milk	1530:1533	arg1	material					1566:1573	a wall material	1559:1573	a wall material	1559:1573	Milk components could act as a wall material and emulsifying agent, protecting the flavonoids from blackberry.
29786840	4	5	theme	other	925:929	arg1	formulations					931:942	other formulations	925:942	other formulations	925:942	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	8	6	theme	intrinsic	1509:1517	arg1	nutrients					1519:1527	intrinsic nutrients	1509:1527	intrinsic nutrients	1509:1527	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	7	7	theme	possible	1344:1351	arg1	dye					1378:1380	a natural dye	1368:1380	a natural dye for food and pharmaceutical products	1368:1417	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	7	7	theme	possible	1344:1351	arg1	application					1353:1363	possible application	1344:1363	possible application	1344:1363	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	4	8	theme	Arabic	753:758	arg1	%					747:747	milk-70% blackberry pulp-5%	721:747	milk-70% blackberry pulp-5% gum Arabic	721:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	6	9	theme	Powder	1035:1040	arg1	stability					1042:1050	Powder stability	1035:1050	Powder stability	1035:1050	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	6	10	theme	content	1124:1130	arg1	values					1083:1088	the critical values	1070:1088	the critical values of relative humidity and moisture content	1070:1130	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	3	11	theme	bulk	463:466	arg1	density					468:474	bulk density	463:474	bulk density	463:474	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	11	theme	bulk	463:466	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	1	12	theme	Spray	158:162	arg1	dryer					164:168	Spray dryer	158:168	Spray dryer	158:168	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	4	13	theme	powder	812:817	arg1	content					828:834	low powder moisture content	808:834	low powder moisture content of 0.0265 g H2 O/g dry matter	808:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	1	14	used	used	174:177	arg2	dryer					164:168	Spray dryer	158:168	Spray dryer	158:168	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	6	15	theme	sorption	1205:1212	arg1	data					1223:1226	sorption isotherm data	1205:1226	sorption isotherm data	1205:1226	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	6	16	theme	humidity	1102:1109	arg1	values					1083:1088	the critical values	1070:1088	the critical values of relative humidity and moisture content	1070:1130	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	0	17	theme	Sorption	114:121	arg1	Properties					86:95	the Physical Properties	73:95	the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature	73:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	7	18	dep	APPLICATION	1239:1249	arg1	due					1291:1293	due	1291:1293	due	1291:1293	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	7	18	dep	APPLICATION	1239:1249	arg1	interest					1263:1270	The growing interest	1251:1270	The growing interest in blackberries	1251:1286	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	6	19	theme	glass	1174:1178	arg1	transition					1180:1189	glass transition	1174:1189	glass transition	1174:1189	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	3	20	theme	water	504:508	arg1	activity					510:517	water activity	504:517	water activity	504:517	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	20	theme	water	504:508	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	1	21	dep	%	239:239	arg1	%					243:243	75%	241:243	25%:75% (w/w)	237:249	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	1	21	dep	%	239:239	arg1	w/w					246:248	w/w	246:248	w/w	246:248	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	7	22	theme	PRACTICAL	1229:1237	arg1	APPLICATION					1239:1249	PRACTICAL APPLICATION	1229:1249	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.	1229:1418	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	4	23	theme	best	648:651	arg1	results					653:659	The best results	644:659	The best results	644:659	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	24	theme	Transition	134:143	arg1	Temperature					145:155	Glass Transition Temperature	128:155	Glass Transition Temperature	128:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	3	25	theme	anthocyanins	544:555	arg1	content					557:563	anthocyanins content	544:563	anthocyanins content	544:563	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	25	theme	anthocyanins	544:555	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	4	26	theme	matter	859:864	arg1	solubility					886:895	a higher powder solubility	870:895	a higher powder solubility (>71.8%)	870:904	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	26	theme	matter	859:864	arg1	%					903:903	>71.8%	898:903	>71.8%	898:903	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	26	theme	matter	859:864	arg1	retention					788:796	high anthocyanins retention	770:796	high anthocyanins retention (>87.5%)	770:805	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	26	theme	matter	859:864	arg1	%					804:804	>87.5%	799:804	>87.5%	799:804	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	26	theme	matter	859:864	arg1	content					828:834	low powder moisture content	808:834	low powder moisture content of 0.0265 g H2 O/g dry matter	808:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	27	theme	Carrier	56:62	arg1	Agent					64:68	Carrier Agent	56:68	Carrier Agent	56:68	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	4	28	theme	0.0265 g	839:846	arg1	matter					859:864	0.0265 g H2 O/g dry matter	839:864	0.0265 g H2 O/g dry matter	839:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	29	theme	paste	683:687	arg1	composition					689:699	the paste composition	679:699	the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic	679:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	30	theme	Physical	77:84	arg1	Properties					86:95	the Physical Properties	73:95	the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature	73:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	5	31	theme	water	961:965	arg1	content					967:973	water content	961:973	water content	961:973	The increase of water content led to a decrease of powder's glass transition temperature.
29786840	4	32	theme	O/g	851:853	arg1	matter					859:864	0.0265 g H2 O/g dry matter	839:864	0.0265 g H2 O/g dry matter	839:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	33	from	Effect	46:51	arg1	Properties					86:95	the Physical Properties	73:95	the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature	73:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	2	34	theme	powder	338:343	arg1	quality					345:351	powder quality	338:351	powder quality	338:351	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	0	35	theme	Milk-Blackberry	16:30	arg1	Mixture					37:43	Milk-Blackberry Pulp Mixture	16:43	Milk-Blackberry Pulp Mixture	16:43	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	7	36	from	interest	1263:1270	arg1	blackberries					1275:1286	blackberries	1275:1286	blackberries	1275:1286	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	4	37	theme	powder	879:884	arg1	solubility					886:895	a higher powder solubility	870:895	a higher powder solubility (>71.8%)	870:904	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	37	theme	powder	879:884	arg1	%					903:903	>71.8%	898:903	>71.8%	898:903	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	38	theme	blackberry	730:739	arg1	%					747:747	milk-70% blackberry pulp-5%	721:747	milk-70% blackberry pulp-5% gum Arabic	721:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	0	39	theme	Mixture	37:43	arg1	Spray-Drying					0:11	Spray-Drying	0:11	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.	0:156	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	7	40	theme	pharmaceutical	1395:1408	arg1	products					1410:1417	pharmaceutical products	1395:1417	pharmaceutical products	1395:1417	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	8	41	theme	pulp	1451:1454	arg1	mixture					1429:1435	a mixture	1427:1435	Drying a mixture of blackberry pulp and milk	1420:1463	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	10	42	theme	postharvesting	1718:1731	arg1	waste					1733:1737	postharvesting waste	1718:1737	postharvesting waste	1718:1737	Therefore, a milk-blackberry powder becomes an alternative, which may reduce postharvesting waste and increase the shelf life of the product without significantly altering its nutritional characteristics.
29786840	2	43	theme	20DE	275:278	arg1	adjuvants					308:316	adjuvants	308:316	adjuvants	308:316	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	43	theme	20DE	275:278	arg1	Arabic					288:293	20DE and gum Arabic	275:293	20DE and gum Arabic	275:293	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	43	theme	20DE	275:278	arg1	10DE					266:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	5	44	theme	transition	1011:1020	arg1	temperature					1022:1032	powder's glass transition temperature	996:1032	powder's glass transition temperature	996:1032	The increase of water content led to a decrease of powder's glass transition temperature.
29786840	8	45	theme	milk	1460:1463	arg1	mixture					1429:1435	a mixture	1427:1435	Drying a mixture of blackberry pulp and milk	1420:1463	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	4	46	theme	%	706:706	arg1	composition					689:699	the paste composition	679:699	the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic	679:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	3	47	from	emphasis	387:394	arg1	activity					510:517	water activity	504:517	water activity	504:517	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	content					557:563	anthocyanins content	544:563	anthocyanins content	544:563	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	content					454:460	moisture content	445:460	moisture content	445:460	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	hygroscopicity					566:579	hygroscopicity	566:579	hygroscopicity	566:579	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	size					486:489	particle size	477:489	particle size	477:489	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	morphology					492:501	morphology	492:501	morphology	492:501	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	retention					533:541	anthocyanins retention	520:541	anthocyanins retention	520:541	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	solubility					582:591	solubility	582:591	solubility	582:591	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	density					468:474	bulk density	463:474	bulk density	463:474	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	47	from	emphasis	387:394	arg1	sorption					600:607	water sorption	594:607	water sorption	594:607	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	8	48	theme	nutrients	1519:1527	arg1	range					1500:1504	a broad range	1492:1504	a broad range of intrinsic nutrients	1492:1527	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	8	49	dep	Drying	1420:1425	arg1	mixture					1429:1435	a mixture	1427:1435	Drying a mixture of blackberry pulp and milk	1420:1463	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	3	50	theme	moisture	445:452	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	50	theme	moisture	445:452	arg1	content					454:460	moisture content	445:460	moisture content	445:460	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	4	51	dep	concentrated	708:719	arg1	%					747:747	milk-70% blackberry pulp-5%	721:747	milk-70% blackberry pulp-5% gum Arabic	721:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	1	52	theme	milk-blackberry	207:221	arg1	mixture					228:234	a milk-blackberry pulp mixture	205:234	a milk-blackberry pulp mixture (25%:75% (w/w))	205:250	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	0	53	dep	Spray-Drying	0:11	arg1	Effect					46:51	Effect	46:51	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.	0:156	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	3	54	theme	transition	620:629	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	54	theme	transition	620:629	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	6	55	theme	state	1156:1160	arg1	diagrams					1162:1169	state diagrams	1156:1169	state diagrams of glass transition combined with sorption isotherm data	1156:1226	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	2	56	theme	gum	284:286	arg1	adjuvants					308:316	adjuvants	308:316	adjuvants	308:316	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	56	theme	gum	284:286	arg1	Arabic					288:293	20DE and gum Arabic	275:293	20DE and gum Arabic	275:293	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	56	theme	gum	284:286	arg1	10DE					266:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	4	57	theme	high	770:773	arg1	retention					788:796	high anthocyanins retention	770:796	high anthocyanins retention (>87.5%)	770:805	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	57	theme	high	770:773	arg1	%					804:804	>87.5%	799:804	>87.5%	799:804	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	8	58	theme	broad	1494:1498	arg1	range					1500:1504	a broad range	1492:1504	a broad range of intrinsic nutrients	1492:1527	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	7	59	theme	anthocyanins	1314:1325	arg1	presence					1302:1309	the presence	1298:1309	the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products	1298:1417	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	10	60	theme	nutritional	1817:1827	arg1	characteristics					1829:1843	its nutritional characteristics	1813:1843	its nutritional characteristics	1813:1843	Therefore, a milk-blackberry powder becomes an alternative, which may reduce postharvesting waste and increase the shelf life of the product without significantly altering its nutritional characteristics.
29786840	6	61	theme	moisture	1115:1122	arg1	content					1124:1130	moisture content	1115:1130	moisture content	1115:1130	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	10	62	theme	milk-blackberry	1654:1668	arg1	powder					1670:1675	a milk-blackberry powder	1652:1675	a milk-blackberry powder	1652:1675	Therefore, a milk-blackberry powder becomes an alternative, which may reduce postharvesting waste and increase the shelf life of the product without significantly altering its nutritional characteristics.
29786840	6	63	theme	isotherm	1214:1221	arg1	data					1223:1226	sorption isotherm data	1205:1226	sorption isotherm data	1205:1226	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	3	64	theme	particle	477:484	arg1	size					486:489	particle size	477:489	particle size	477:489	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	64	theme	particle	477:484	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	4	65	theme	low	808:810	arg1	content					828:834	low powder moisture content	808:834	low powder moisture content of 0.0265 g H2 O/g dry matter	808:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	66	theme	moisture	819:826	arg1	content					828:834	low powder moisture content	808:834	low powder moisture content of 0.0265 g H2 O/g dry matter	808:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	6	67	theme	relative	1093:1100	arg1	humidity					1102:1109	relative humidity	1093:1109	relative humidity	1093:1109	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	6	68	theme	transition	1180:1189	arg1	diagrams					1162:1169	state diagrams	1156:1169	state diagrams of glass transition combined with sorption isotherm data	1156:1226	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	9	69	theme	wall	1561:1564	arg1	components					1535:1544	Milk components	1530:1544	Milk components	1530:1544	Milk components could act as a wall material and emulsifying agent, protecting the flavonoids from blackberry.
29786840	9	69	theme	wall	1561:1564	arg1	material					1566:1573	a wall material	1559:1573	a wall material	1559:1573	Milk components could act as a wall material and emulsifying agent, protecting the flavonoids from blackberry.
29786840	0	70	theme	Water	108:112	arg1	Sorption					114:121	Water Sorption	108:121	Water Sorption	108:121	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	7	71	theme	growing	1255:1261	arg1	due					1291:1293	due	1291:1293	due	1291:1293	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	7	71	theme	growing	1255:1261	arg1	interest					1263:1270	The growing interest	1251:1270	The growing interest in blackberries	1251:1286	PRACTICAL APPLICATION The growing interest in blackberries is due to the presence of anthocyanins which have shown possible application as a natural dye for food and pharmaceutical products.
29786840	0	72	theme	Temperature	145:155	arg1	Properties					86:95	the Physical Properties	73:95	the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature	73:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	1	73	dep	mixture	228:234	arg1	%					239:239	25%	237:239	25%:75% (w/w)	237:249	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
29786840	2	74	used	used	300:303	arg2	Arabic					288:293	20DE and gum Arabic	275:293	20DE and gum Arabic	275:293	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	74	used	used	300:303	arg2	10DE					266:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	74	used	used	300:303	arg2	adjuvants					308:316	adjuvants	308:316	adjuvants	308:316	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	0	75	theme	Agent	64:68	arg1	Effect					46:51	Effect	46:51	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.	0:156	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	3	76	theme	anthocyanins	520:531	arg1	retention					533:541	anthocyanins retention	520:541	anthocyanins retention	520:541	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	76	theme	anthocyanins	520:531	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	4	77	theme	dry	855:857	arg1	matter					859:864	0.0265 g H2 O/g dry matter	839:864	0.0265 g H2 O/g dry matter	839:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	2	78	theme	Maltodextrin	253:264	arg1	adjuvants					308:316	adjuvants	308:316	adjuvants	308:316	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	78	theme	Maltodextrin	253:264	arg1	Arabic					288:293	20DE and gum Arabic	275:293	20DE and gum Arabic	275:293	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	2	78	theme	Maltodextrin	253:264	arg1	10DE					266:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE	253:269	Maltodextrin 10DE and 20DE and gum Arabic were used as adjuvants in order to improve powder quality.
29786840	0	79	theme	Powder	100:105	arg1	Properties					86:95	the Physical Properties	73:95	the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature	73:155	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	5	80	theme	content	967:973	arg1	increase					949:956	The increase	945:956	The increase of water content	945:973	The increase of water content led to a decrease of powder's glass transition temperature.
29786840	4	81	theme	H2	848:849	arg1	matter					859:864	0.0265 g H2 O/g dry matter	839:864	0.0265 g H2 O/g dry matter	839:864	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	3	82	dep	characteristics	428:442	arg1	activity					510:517	water activity	504:517	water activity	504:517	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	content					557:563	anthocyanins content	544:563	anthocyanins content	544:563	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	content					454:460	moisture content	445:460	moisture content	445:460	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	hygroscopicity					566:579	hygroscopicity	566:579	hygroscopicity	566:579	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	size					486:489	particle size	477:489	particle size	477:489	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	morphology					492:501	morphology	492:501	morphology	492:501	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	retention					533:541	anthocyanins retention	520:541	anthocyanins retention	520:541	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	solubility					582:591	solubility	582:591	solubility	582:591	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	density					468:474	bulk density	463:474	bulk density	463:474	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	82	dep	characteristics	428:442	arg1	sorption					600:607	water sorption	594:607	water sorption	594:607	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	0	83	theme	Pulp	32:35	arg1	Mixture					37:43	Milk-Blackberry Pulp Mixture	16:43	Milk-Blackberry Pulp Mixture	16:43	Spray-Drying of Milk-Blackberry Pulp Mixture: Effect of Carrier Agent on the Physical Properties of Powder, Water Sorption, and Glass Transition Temperature.
29786840	8	84	theme	blackberry	1440:1449	arg1	pulp					1451:1454	blackberry pulp	1440:1454	blackberry pulp	1440:1454	Drying a mixture of blackberry pulp and milk may produce particles with a broad range of intrinsic nutrients.
29786840	4	85	theme	higher	872:877	arg1	solubility					886:895	a higher powder solubility	870:895	a higher powder solubility (>71.8%)	870:904	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	85	theme	higher	872:877	arg1	%					903:903	>71.8%	898:903	>71.8%	898:903	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	4	86	theme	%	728:728	arg1	%					747:747	milk-70% blackberry pulp-5%	721:747	milk-70% blackberry pulp-5% gum Arabic	721:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	5	87	theme	glass	1005:1009	arg1	temperature					1022:1032	powder's glass transition temperature	996:1032	powder's glass transition temperature	996:1032	The increase of water content led to a decrease of powder's glass transition temperature.
29786840	9	88	theme	emulsifying	1579:1589	arg1	agent					1591:1595	emulsifying agent	1579:1595	emulsifying agent	1579:1595	Milk components could act as a wall material and emulsifying agent, protecting the flavonoids from blackberry.
29786840	3	89	theme	water	594:598	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	89	theme	water	594:598	arg1	sorption					600:607	water sorption	594:607	water sorption	594:607	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	activity					510:517	water activity	504:517	water activity	504:517	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	content					557:563	anthocyanins content	544:563	anthocyanins content	544:563	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	content					454:460	moisture content	445:460	moisture content	445:460	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	hygroscopicity					566:579	hygroscopicity	566:579	hygroscopicity	566:579	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	size					486:489	particle size	477:489	particle size	477:489	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	morphology					492:501	morphology	492:501	morphology	492:501	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	retention					533:541	anthocyanins retention	520:541	anthocyanins retention	520:541	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	solubility					582:591	solubility	582:591	solubility	582:591	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	density					468:474	bulk density	463:474	bulk density	463:474	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	90	theme	physicochemical	412:426	arg1	sorption					600:607	water sorption	594:607	water sorption	594:607	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	5	91	theme	temperature	1022:1032	arg1	decrease					984:991	a decrease	982:991	a decrease of powder's glass transition temperature	982:1032	The increase of water content led to a decrease of powder's glass transition temperature.
29786840	10	92	theme	shelf	1756:1760	arg1	life					1762:1765	the shelf life	1752:1765	the shelf life of the product	1752:1780	Therefore, a milk-blackberry powder becomes an alternative, which may reduce postharvesting waste and increase the shelf life of the product without significantly altering its nutritional characteristics.
29786840	3	93	theme	physical	399:406	arg1	activity					510:517	water activity	504:517	water activity	504:517	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	content					557:563	anthocyanins content	544:563	anthocyanins content	544:563	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	content					454:460	moisture content	445:460	moisture content	445:460	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	hygroscopicity					566:579	hygroscopicity	566:579	hygroscopicity	566:579	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	size					486:489	particle size	477:489	particle size	477:489	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	morphology					492:501	morphology	492:501	morphology	492:501	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	retention					533:541	anthocyanins retention	520:541	anthocyanins retention	520:541	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	solubility					582:591	solubility	582:591	solubility	582:591	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	density					468:474	bulk density	463:474	bulk density	463:474	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	93	theme	physical	399:406	arg1	sorption					600:607	water sorption	594:607	water sorption	594:607	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	94	theme	glass	614:618	arg1	characteristics					428:442	physical and physicochemical characteristics	399:442	physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature	399:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	3	94	theme	glass	614:618	arg1	temperature					631:641	glass transition temperature	614:641	glass transition temperature	614:641	The particles were analyzed with emphasis on physical and physicochemical characteristics: moisture content, bulk density, particle size, morphology, water activity, anthocyanins retention, anthocyanins content, hygroscopicity, solubility, water sorption, and glass transition temperature.
29786840	4	95	theme	concentrated	708:719	arg1	%					706:706	25%	704:706	25% concentrated milk-70% blackberry pulp-5% gum Arabic	704:758	The best results were obtained for the paste composition of 25% concentrated milk-70% blackberry pulp-5% gum Arabic, yielding high anthocyanins retention (>87.5%), low powder moisture content of 0.0265 g H2 O/g dry matter and a higher powder solubility (>71.8%) when compared with other formulations.
29786840	6	96	theme	critical	1074:1081	arg1	values					1083:1088	the critical values	1070:1088	the critical values of relative humidity and moisture content	1070:1130	Powder stability was evaluated and the critical values of relative humidity and moisture content were estimated based on state diagrams of glass transition combined with sorption isotherm data.
29786840	1	97	theme	pulp	223:226	arg1	mixture					228:234	a milk-blackberry pulp mixture	205:234	a milk-blackberry pulp mixture (25%:75% (w/w))	205:250	Spray dryer was used to produce particles from a milk-blackberry pulp mixture (25%:75% (w/w)).
31250530	4	0	theme	dermatan	931:938	arg1	sulfate					940:946	dermatan sulfate	931:946	dermatan sulfate	931:946	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	6	1	theme	chondroitin	1345:1355	arg1	sulfate					1357:1363	chondroitin sulfate	1345:1363	chondroitin sulfate	1345:1363	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	5	2	with	media	1097:1101	arg1	accuracy					1193:1200	highly acceptable accuracy	1175:1200	highly acceptable accuracy	1175:1200	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	2	with	media	1097:1101	arg1	precision					1206:1214	precision	1206:1214	precision	1206:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	1	3	theme	chondroitin	304:314	arg1	sulfate					316:322	chondroitin sulfate	304:322	chondroitin sulfate	304:322	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	6	4	theme	capillary	1241:1249	arg1	electrophoresis					1251:1265	the proposed capillary electrophoresis	1228:1265	the proposed capillary electrophoresis approach	1228:1274	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	4	5	theme	flow	736:739	arg1	pump					741:744	an electroosmotic flow pump	718:744	an electroosmotic flow pump	718:744	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	3	6	theme	w/v	602:604	arg1	glycol					619:624	0.5% w/v polyethylene glycol	597:624	0.5% w/v polyethylene glycol	597:624	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	5	7	from	sulfate	1053:1059	arg1	fluid					1118:1122	cerebrospinal fluid	1104:1122	cerebrospinal fluid	1104:1122	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	7	from	sulfate	1053:1059	arg1	products					1134:1141	cosmetic products	1125:1141	cosmetic products	1125:1141	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	7	from	sulfate	1053:1059	arg1	samples					1162:1168	supplementary samples	1148:1168	supplementary samples	1148:1168	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	7	from	sulfate	1053:1059	arg1	media					1097:1101	cell culture media	1084:1101	cell culture media	1084:1101	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	1	8	theme	large-volume	202:213	arg1	stacking					222:229	large-volume sample stacking	202:229	large-volume sample stacking using an electroosmotic flow pump	202:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	0	9	theme	cosmetic	121:128	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	10	theme	cosmetic	1125:1132	arg1	products					1134:1141	cosmetic products	1125:1141	cosmetic products	1125:1141	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	1	11	theme	flow	255:258	arg1	pump					260:263	an electroosmotic flow pump	237:263	an electroosmotic flow pump	237:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	4	12	theme	hyaluronic	953:962	arg1	acid					964:967	hyaluronic acid	953:967	hyaluronic acid	953:967	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	4	13	theme	capillary	779:787	arg1	system					805:810	the proposed capillary electrophoresis system	766:810	the proposed capillary electrophoresis system coupled with UV detection	766:836	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	6	14	theme	sulfate	1375:1381	arg1	determination					1328:1340	the determination	1324:1340	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment	1324:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	4	15	theme	system	805:810	arg1	sensitivity					751:761	the sensitivity	747:761	the sensitivity of the proposed capillary electrophoresis system coupled with UV detection	747:836	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	5	16	theme	developed	988:996	arg1	method					998:1003	The developed method	984:1003	The developed method	984:1003	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	17	from	acid	98:101	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	18	with	samples	1162:1168	arg1	accuracy					1193:1200	highly acceptable accuracy	1175:1200	highly acceptable accuracy	1175:1200	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	18	with	samples	1162:1168	arg1	precision					1206:1214	precision	1206:1214	precision	1206:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	19	theme	dermatan	66:73	arg1	sulfate					75:81	dermatan sulfate	66:81	dermatan sulfate	66:81	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	20	theme	acceptable	1182:1191	arg1	accuracy					1193:1200	highly acceptable accuracy	1175:1200	highly acceptable accuracy	1175:1200	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	21	theme	cerebrospinal	1427:1439	arg1	media					1420:1424	cell culture media	1407:1424	cell culture media	1407:1424	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	21	theme	cerebrospinal	1427:1439	arg1	fluid					1441:1445	cerebrospinal fluid	1427:1445	cerebrospinal fluid	1427:1445	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	1	22	with	electrophoresis	181:195	arg1	stacking					222:229	large-volume sample stacking	202:229	large-volume sample stacking using an electroosmotic flow pump	202:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	2	23	used	used	393:396	arg2	design					382:387	Central composite design	364:387	Central composite design	364:387	Central composite design was used to simultaneously optimize the parameters for capillary electrophoresis separation.
31250530	0	24	theme	acid	98:101	arg1	determination					28:40	determination	28:40	determination	28:40	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	24	theme	acid	98:101	arg1	preconcentration					7:22	Online preconcentration	0:22	Online preconcentration	0:22	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	25	theme	supplementary	1148:1160	arg1	samples					1162:1168	supplementary samples	1148:1168	supplementary samples	1148:1168	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	26	from	sulfate	57:63	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	3	27	theme	capillary	496:504	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	27	theme	capillary	496:504	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	0	28	theme	biological	106:115	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	29	theme	chondroitin	1041:1051	arg1	sulfate					1053:1059	chondroitin sulfate	1041:1059	chondroitin sulfate	1041:1059	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	30	theme	sulfate	75:81	arg1	determination					28:40	determination	28:40	determination	28:40	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	30	theme	sulfate	75:81	arg1	preconcentration					7:22	Online preconcentration	0:22	Online preconcentration	0:22	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	31	theme	Online	0:5	arg1	preconcentration					7:22	Online preconcentration	0:22	Online preconcentration	0:22	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	1	32	theme	sulfate	316:322	arg1	determination					287:299	the determination	283:299	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid	283:361	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	3	33	theme	200 mM	538:543	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	33	theme	200 mM	538:543	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	5	34	from	samples	1162:1168	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	1	35	theme	dermatan	325:332	arg1	sulfate					334:340	dermatan sulfate	325:340	dermatan sulfate	325:340	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	5	36	with	products	1134:1141	arg1	accuracy					1193:1200	highly acceptable accuracy	1175:1200	highly acceptable accuracy	1175:1200	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	36	with	products	1134:1141	arg1	precision					1206:1214	precision	1206:1214	precision	1206:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	2	37	theme	electrophoresis	454:468	arg1	separation					470:479	capillary electrophoresis separation	444:479	capillary electrophoresis separation	444:479	Central composite design was used to simultaneously optimize the parameters for capillary electrophoresis separation.
31250530	6	38	theme	supplementary	1462:1474	arg1	media					1420:1424	cell culture media	1407:1424	cell culture media	1407:1424	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	38	theme	supplementary	1462:1474	arg1	samples					1476:1482	supplementary samples	1462:1482	supplementary samples without sample pretreatment	1462:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	5	39	theme	cell	1084:1087	arg1	media					1097:1101	cell culture media	1084:1101	cell culture media	1084:1101	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	40	theme	sulfate	57:63	arg1	determination					28:40	determination	28:40	determination	28:40	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	40	theme	sulfate	57:63	arg1	preconcentration					7:22	Online preconcentration	0:22	Online preconcentration	0:22	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	1	41	theme	hyaluronic	347:356	arg1	acid					358:361	hyaluronic acid	347:361	hyaluronic acid	347:361	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	2	42	theme	Central	364:370	arg1	design					382:387	Central composite design	364:387	Central composite design	364:387	Central composite design was used to simultaneously optimize the parameters for capillary electrophoresis separation.
31250530	2	43	theme	capillary	444:452	arg1	separation					470:479	capillary electrophoresis separation	444:479	capillary electrophoresis separation	444:479	Central composite design was used to simultaneously optimize the parameters for capillary electrophoresis separation.
31250530	5	44	theme	acid	1076:1079	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	45	theme	sulfate	1357:1363	arg1	determination					1328:1340	the determination	1324:1340	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment	1324:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	0	46	from	samples	130:136	arg1	determination					28:40	determination	28:40	determination	28:40	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	46	from	samples	130:136	arg1	preconcentration					7:22	Online preconcentration	0:22	Online preconcentration	0:22	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	3	47	theme	polyethylene	606:617	arg1	glycol					619:624	0.5% w/v polyethylene glycol	597:624	0.5% w/v polyethylene glycol	597:624	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	5	48	from	determination	1024:1036	arg1	fluid					1118:1122	cerebrospinal fluid	1104:1122	cerebrospinal fluid	1104:1122	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	48	from	determination	1024:1036	arg1	products					1134:1141	cosmetic products	1125:1141	cosmetic products	1125:1141	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	48	from	determination	1024:1036	arg1	samples					1162:1168	supplementary samples	1148:1168	supplementary samples	1148:1168	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	48	from	determination	1024:1036	arg1	media					1097:1101	cell culture media	1084:1101	cell culture media	1084:1101	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	49	theme	electrophoresis	1251:1265	arg1	approach					1267:1274	the proposed capillary electrophoresis approach	1228:1274	the proposed capillary electrophoresis approach	1228:1274	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	3	50	theme	%	600:600	arg1	glycol					619:624	0.5% w/v polyethylene glycol	597:624	0.5% w/v polyethylene glycol	597:624	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	5	51	from	acid	1076:1079	arg1	fluid					1118:1122	cerebrospinal fluid	1104:1122	cerebrospinal fluid	1104:1122	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	51	from	acid	1076:1079	arg1	products					1134:1141	cosmetic products	1125:1141	cosmetic products	1125:1141	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	51	from	acid	1076:1079	arg1	samples					1162:1168	supplementary samples	1148:1168	supplementary samples	1148:1168	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	51	from	acid	1076:1079	arg1	media					1097:1101	cell culture media	1084:1101	cell culture media	1084:1101	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	52	theme	cell	1407:1410	arg1	media					1420:1424	cell culture media	1407:1424	cell culture media	1407:1424	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	52	theme	cell	1407:1410	arg1	fluid					1441:1445	cerebrospinal fluid	1427:1445	cerebrospinal fluid	1427:1445	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	52	theme	cell	1407:1410	arg1	samples					1476:1482	supplementary samples	1462:1482	supplementary samples without sample pretreatment	1462:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	52	theme	cell	1407:1410	arg1	cosmetic					1448:1455	cosmetic	1448:1455	cosmetic	1448:1455	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	53	theme	proposed	1232:1239	arg1	electrophoresis					1251:1265	the proposed capillary electrophoresis	1228:1265	the proposed capillary electrophoresis approach	1228:1274	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	4	54	theme	electroosmotic	721:734	arg1	pump					741:744	an electroosmotic flow pump	718:744	an electroosmotic flow pump	718:744	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	3	55	theme	background	631:640	arg1	pH					655:656	pH 4	655:658	pH 4	655:658	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	55	theme	background	631:640	arg1	electrolyte					642:652	a background electrolyte	629:652	a background electrolyte	629:652	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	1	56	theme	sample	215:220	arg1	stacking					222:229	large-volume sample stacking	202:229	large-volume sample stacking using an electroosmotic flow pump	202:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	4	57	theme	chondroitin	910:920	arg1	sulfate					922:928	chondroitin sulfate	910:928	chondroitin sulfate	910:928	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	5	58	from	media	1097:1101	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	4	59	theme	sample	696:701	arg1	stacking					703:710	large-volume sample stacking	683:710	large-volume sample stacking using an electroosmotic flow pump	683:744	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	2	60	theme	composite	372:380	arg1	design					382:387	Central composite design	364:387	Central composite design	364:387	Central composite design was used to simultaneously optimize the parameters for capillary electrophoresis separation.
31250530	0	61	theme	capillary	144:152	arg1	electrophoresis					154:168	capillary electrophoresis	144:168	capillary electrophoresis	144:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	1	62	theme	electroosmotic	240:253	arg1	pump					260:263	an electroosmotic flow pump	237:263	an electroosmotic flow pump	237:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	0	63	from	sulfate	75:81	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	4	64	theme	proposed	770:777	arg1	system					805:810	the proposed capillary electrophoresis system	766:810	the proposed capillary electrophoresis system coupled with UV detection	766:836	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	6	65	theme	dermatan	1366:1373	arg1	sulfate					1375:1381	dermatan sulfate	1366:1381	dermatan sulfate	1366:1381	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	5	66	theme	cerebrospinal	1104:1116	arg1	fluid					1118:1122	cerebrospinal fluid	1104:1122	cerebrospinal fluid	1104:1122	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	4	67	theme	electrophoresis	789:803	arg1	system					805:810	the proposed capillary electrophoresis system	766:810	the proposed capillary electrophoresis system coupled with UV detection	766:836	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	3	68	theme	dihydrogen	552:561	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	68	theme	dihydrogen	552:561	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	5	69	from	fluid	1118:1122	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	0	70	from	determination	28:40	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	0	71	theme	hyaluronic	87:96	arg1	acid					98:101	hyaluronic acid	87:101	hyaluronic acid	87:101	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	1	72	theme	Capillary	171:179	arg1	electrophoresis					181:195	Capillary electrophoresis	171:195	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump	171:263	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	6	73	theme	acid	1399:1402	arg1	determination					1328:1340	the determination	1324:1340	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment	1324:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	0	74	from	preconcentration	7:22	arg1	samples					130:136	biological and cosmetic samples	106:136	biological and cosmetic samples using capillary electrophoresis	106:168	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	5	75	with	fluid	1118:1122	arg1	accuracy					1193:1200	highly acceptable accuracy	1175:1200	highly acceptable accuracy	1175:1200	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	75	with	fluid	1118:1122	arg1	precision					1206:1214	precision	1206:1214	precision	1206:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	76	theme	culture	1412:1418	arg1	media					1420:1424	cell culture media	1407:1424	cell culture media	1407:1424	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	76	theme	culture	1412:1418	arg1	fluid					1441:1445	cerebrospinal fluid	1427:1445	cerebrospinal fluid	1427:1445	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	76	theme	culture	1412:1418	arg1	samples					1476:1482	supplementary samples	1462:1482	supplementary samples without sample pretreatment	1462:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	76	theme	culture	1412:1418	arg1	cosmetic					1448:1455	cosmetic	1448:1455	cosmetic	1448:1455	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	3	77	theme	electrophoresis	506:520	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	77	theme	electrophoresis	506:520	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	6	78	theme	hyaluronic	1388:1397	arg1	acid					1399:1402	hyaluronic acid	1388:1402	hyaluronic acid	1388:1402	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	3	79	theme	optimized	486:494	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	79	theme	optimized	486:494	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	6	80	theme	sample	1492:1497	arg1	pretreatment					1499:1510	sample pretreatment	1492:1510	sample pretreatment	1492:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	4	81	theme	UV	825:826	arg1	detection					828:836	UV detection	825:836	UV detection	825:836	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	3	82	theme	sodium	545:550	arg1	phosphate					563:571	200 mM sodium dihydrogen phosphate	538:571	200 mM sodium dihydrogen phosphate	538:571	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	3	82	theme	sodium	545:550	arg1	conditions					522:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions	482:531	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	0	83	theme	chondroitin	45:55	arg1	sulfate					57:63	chondroitin sulfate	45:63	chondroitin sulfate	45:63	Online preconcentration and determination of chondroitin sulfate, dermatan sulfate and hyaluronic acid in biological and cosmetic samples using capillary electrophoresis.
31250530	1	84	theme	sulfate	334:340	arg1	determination					287:299	the determination	283:299	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid	283:361	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	4	85	theme	3	893:893	arg1	detection					880:888	detection	880:888	detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively	880:981	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	5	86	from	products	1134:1141	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	5	87	theme	culture	1089:1095	arg1	media					1097:1101	cell culture media	1084:1101	cell culture media	1084:1101	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	3	88	theme	200 mM	574:579	arg1	butylamine					581:590	200 mM butylamine	574:590	200 mM butylamine	574:590	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31250530	1	89	theme	acid	358:361	arg1	determination					287:299	the determination	283:299	the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid	283:361	Capillary electrophoresis with large-volume sample stacking using an electroosmotic flow pump was developed for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid.
31250530	5	90	theme	sulfate	1053:1059	arg1	determination					1024:1036	the determination	1020:1036	the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision	1020:1214	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	6	91	from	determination	1328:1340	arg1	media					1420:1424	cell culture media	1407:1424	cell culture media	1407:1424	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	91	from	determination	1328:1340	arg1	fluid					1441:1445	cerebrospinal fluid	1427:1445	cerebrospinal fluid	1427:1445	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	91	from	determination	1328:1340	arg1	samples					1476:1482	supplementary samples	1462:1482	supplementary samples without sample pretreatment	1462:1510	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	6	91	from	determination	1328:1340	arg1	cosmetic					1448:1455	cosmetic	1448:1455	cosmetic	1448:1455	Therefore, the proposed capillary electrophoresis approach was found to be simple, rapid, and reliable for the determination of chondroitin sulfate, dermatan sulfate, and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic, and supplementary samples without sample pretreatment.
31250530	5	92	theme	hyaluronic	1065:1074	arg1	acid					1076:1079	hyaluronic acid	1065:1079	hyaluronic acid	1065:1079	The developed method was applied to the determination of chondroitin sulfate and hyaluronic acid in cell culture media, cerebrospinal fluid, cosmetic products, and supplementary samples with highly acceptable accuracy and precision.
31250530	4	93	theme	large-volume	683:694	arg1	stacking					703:710	large-volume sample stacking	683:710	large-volume sample stacking using an electroosmotic flow pump	683:744	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	4	94	theme	detection	880:888	arg1	limits					870:875	limits	870:875	limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively	870:981	Exploiting large-volume sample stacking using an electroosmotic flow pump, the sensitivity of the proposed capillary electrophoresis system coupled with UV detection was significantly improved with limits of detection of 3, 5, 1 mg/L for chondroitin sulfate, dermatan sulfate, and hyaluronic acid, respectively.
31250530	3	95	theme	0.5	597:599	arg1	%					600:600	%	600:600	%	600:600	The optimized capillary electrophoresis conditions were 200 mM sodium dihydrogen phosphate, 200 mM butylamine, and 0.5% w/v polyethylene glycol as a background electrolyte, pH 4 and -16 kV.
31067487	6	0	theme	EPS	948:950	arg1	spectra					923:929	The thermogram profile and SEM-EDX spectra	888:929	spectra	923:929	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	0	theme	EPS	948:950	arg1	profile					903:909	The thermogram profile and SEM-EDX spectra	888:929	profile	903:909	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	2	1	theme	media	381:385	arg1	components					387:396	media components	381:396	media components using Response surface methodology	381:431	The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology.
31067487	1	2	theme	probiont	266:273	arg1	GD5					301:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
31067487	0	3	theme	exopolysaccharide	83:99	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	6	4	theme	extracted	938:946	arg1	EPS					948:950	the extracted EPS	934:950	the extracted EPS	934:950	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	7	5	dep	revealed	1127:1134	arg1	corroborated					1205:1216	corroborated	1205:1216	corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement	1205:1295	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	1	6	theme	Streptomyces	275:286	arg1	GD5					301:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
31067487	0	7	theme	Streptomyces	116:127	arg1	GD5					142:144	probiotic Streptomyces griseorubens GD5	106:144	probiotic Streptomyces griseorubens GD5	106:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	4	8	theme	FT-IR	566:570	arg1	hydroxyl					614:621	hydroxyl	614:621	hydroxyl	614:621	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	8	theme	FT-IR	566:570	arg1	groups					644:649	amide groups	638:649	amide groups	638:649	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	8	theme	FT-IR	566:570	arg1	linkages					604:611	β-glycosidic linkages	591:611	β-glycosidic linkages	591:611	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	8	theme	FT-IR	566:570	arg1	carboxyl					624:631	carboxyl	624:631	carboxyl	624:631	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	8	theme	FT-IR	566:570	arg1	spectra					572:578	FT-IR spectra	566:578	FT-IR spectra	566:578	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	5	9	theme	NMR	703:705	arg1	spectra					707:713	NMR spectra	703:713	NMR spectra	703:713	Glucose, galactose, and mannose were detected from NMR spectra whilst the occurrence of arabinose, glucose, galactose, mannose, and xylose, were detected from HPLC chromatogram, signifying that GD5EPS is heteropolysaccharide in nature.
31067487	1	10	theme	griseorubens	288:299	arg1	GD5					301:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
31067487	0	11	theme	probiotic	106:114	arg1	GD5					142:144	probiotic Streptomyces griseorubens GD5	106:144	probiotic Streptomyces griseorubens GD5	106:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	4	12	theme	functional	531:540	arg1	groups					542:547	Several functional groups	523:547	Several functional groups	523:547	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	6	13	with	thermo-stable	981:993	arg1	units					1043:1047	flake-like structural units	1021:1047	flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na	1021:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	2	14	theme	EPS	310:312	arg1	production					314:323	The EPS production	306:323	The EPS production	306:323	The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology.
31067487	4	15	theme	amide	638:642	arg1	linkages					604:611	β-glycosidic linkages	591:611	β-glycosidic linkages	591:611	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	15	theme	amide	638:642	arg1	groups					644:649	amide groups	638:649	amide groups	638:649	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	15	theme	amide	638:642	arg1	spectra					572:578	FT-IR spectra	566:578	FT-IR spectra	566:578	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	0	16	from	attributes	61:70	arg1	GD5					142:144	probiotic Streptomyces griseorubens GD5	106:144	probiotic Streptomyces griseorubens GD5	106:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	6	17	theme	Mg	1092:1093	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	18	theme	P	1089:1089	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	3	19	theme	GD5EPS	468:473	arg1	properties					454:463	The physio-chemical properties	434:463	The physio-chemical properties of GD5EPS	434:473	The physio-chemical properties of GD5EPS were examined by various analytical techniques.
31067487	1	20	theme	bio-functional	178:191	arg1	attributes					193:202	the bio-functional attributes	174:202	the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5	174:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
31067487	0	21	theme	griseorubens	129:140	arg1	GD5					142:144	probiotic Streptomyces griseorubens GD5	106:144	probiotic Streptomyces griseorubens GD5	106:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	6	22	theme	SEM-EDX	915:921	arg1	spectra					923:929	The thermogram profile and SEM-EDX spectra	888:929	spectra	923:929	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	23	theme	flake-like	1021:1030	arg1	units					1043:1047	flake-like structural units	1021:1047	flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na	1021:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	7	24	theme	antioxidant	1146:1156	arg1	efficacies					1187:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	0	25	theme	optimization	12:23	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	4	26	theme	Several	523:529	arg1	groups					542:547	Several functional groups	523:547	Several functional groups	523:547	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	2	27	theme	surface	413:419	arg1	methodology					421:431	Response surface methodology	404:431	Response surface methodology	404:431	The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology.
31067487	0	28	theme	Statistical	0:10	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	2	29	theme	Response	404:411	arg1	methodology					421:431	Response surface methodology	404:431	Response surface methodology	404:431	The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology.
31067487	0	30	theme	physio-chemical	26:40	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	5	31	theme	arabinose	740:748	arg1	occurrence					726:735	the occurrence	722:735	the occurrence of arabinose, glucose, galactose, mannose, and xylose,	722:790	Glucose, galactose, and mannose were detected from NMR spectra whilst the occurrence of arabinose, glucose, galactose, mannose, and xylose, were detected from HPLC chromatogram, signifying that GD5EPS is heteropolysaccharide in nature.
31067487	1	32	theme	exopolysaccharide	210:226	arg1	attributes					193:202	the bio-functional attributes	174:202	the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5	174:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
31067487	6	33	theme	Na	1107:1108	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	7	34	theme	food	1281:1284	arg1	EPS					1223:1225	EPS	1223:1225	EPS derived from S. griseorubens GD5	1223:1258	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	7	34	theme	food	1281:1284	arg1	supplement					1286:1295	a promising food supplement	1269:1295	a promising food supplement	1269:1295	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	0	35	theme	bio-functional	46:59	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	6	36	theme	thermogram	892:901	arg1	profile					903:909	The thermogram profile and SEM-EDX spectra	888:929	profile	903:909	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	37	with	units	1043:1047	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	3	38	theme	various	492:498	arg1	techniques					511:520	various analytical techniques	492:520	various analytical techniques	492:520	The physio-chemical properties of GD5EPS were examined by various analytical techniques.
31067487	6	39	theme	O	1086:1086	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	3	40	theme	analytical	500:509	arg1	techniques					511:520	various analytical techniques	492:520	various analytical techniques	492:520	The physio-chemical properties of GD5EPS were examined by various analytical techniques.
31067487	5	41	theme	HPLC	811:814	arg1	chromatogram					816:827	HPLC chromatogram	811:827	HPLC chromatogram	811:827	Glucose, galactose, and mannose were detected from NMR spectra whilst the occurrence of arabinose, glucose, galactose, mannose, and xylose, were detected from HPLC chromatogram, signifying that GD5EPS is heteropolysaccharide in nature.
31067487	4	42	located	detected	554:561	arg1	groups					644:649	amide groups	638:649	amide groups	638:649	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	42	located	detected	554:561	arg2	groups					542:547	Several functional groups	523:547	Several functional groups	523:547	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	42	located	detected	554:561	arg1	hydroxyl					614:621	hydroxyl	614:621	hydroxyl	614:621	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	42	located	detected	554:561	arg1	spectra					572:578	FT-IR spectra	566:578	FT-IR spectra	566:578	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	42	located	detected	554:561	arg1	carboxyl					624:631	carboxyl	624:631	carboxyl	624:631	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	4	42	located	detected	554:561	arg1	linkages					604:611	β-glycosidic linkages	591:611	β-glycosidic linkages	591:611	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	3	43	theme	physio-chemical	438:452	arg1	properties					454:463	The physio-chemical properties	434:463	The physio-chemical properties of GD5EPS	434:473	The physio-chemical properties of GD5EPS were examined by various analytical techniques.
31067487	7	44	attach	derived	1227:1233	arg1	GD5					1256:1258	GD5	1256:1258	GD5	1256:1258	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	7	44	attach	derived	1227:1233	arg2	supplement					1286:1295	a promising food supplement	1269:1295	a promising food supplement	1269:1295	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	7	44	attach	derived	1227:1233	arg2	EPS					1223:1225	EPS	1223:1225	EPS derived from S. griseorubens GD5	1223:1258	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	7	45	theme	emulsifying	1159:1169	arg1	efficacies					1187:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	0	46	from	GD5	142:144	arg1	exopolysaccharide					83:99	a novel exopolysaccharide	75:99	a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5	75:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	0	46	from	GD5	142:144	arg1	attributes					61:70	Statistical optimization, physio-chemical and bio-functional attributes	0:70	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.	0:145	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	7	47	theme	antibiofilm	1175:1185	arg1	efficacies					1187:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	4	48	theme	β-glycosidic	591:602	arg1	linkages					604:611	β-glycosidic linkages	591:611	β-glycosidic linkages	591:611	Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups.
31067487	5	49	from	heteropolysaccharide	856:875	arg1	nature					880:885	nature	880:885	nature	880:885	Glucose, galactose, and mannose were detected from NMR spectra whilst the occurrence of arabinose, glucose, galactose, mannose, and xylose, were detected from HPLC chromatogram, signifying that GD5EPS is heteropolysaccharide in nature.
31067487	6	50	theme	Ca	1099:1100	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	7	51	theme	potential	1136:1144	arg1	efficacies					1187:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	potential antioxidant, emulsifying and antibiofilm efficacies	1136:1196	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	0	52	theme	novel	77:81	arg1	exopolysaccharide					83:99	a novel exopolysaccharide	75:99	a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5	75:144	Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
31067487	6	53	theme	elemental	1058:1066	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	54	theme	C	1083:1083	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	6	55	theme	K	1096:1096	arg1	composition					1068:1078	the elemental composition	1054:1078	the elemental composition of C, O, P, Mg, K, Ca, and Na	1054:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	7	56	theme	promising	1271:1279	arg1	EPS					1223:1225	EPS	1223:1225	EPS derived from S. griseorubens GD5	1223:1258	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	7	56	theme	promising	1271:1279	arg1	supplement					1286:1295	a promising food supplement	1269:1295	a promising food supplement	1269:1295	The GD5EPS also revealed potential antioxidant, emulsifying and antibiofilm efficacies, which corroborated that EPS derived from S. griseorubens GD5 might be a promising food supplement.
31067487	6	57	theme	structural	1032:1041	arg1	units					1043:1047	flake-like structural units	1021:1047	flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na	1021:1108	The thermogram profile and SEM-EDX spectra of the extracted EPS indicated that it was highly thermo-stable, compact and porous, with flake-like structural units with the elemental composition of C, O, P, Mg, K, Ca, and Na.
31067487	2	58	theme	components	387:396	arg1	concentration					364:376	the concentration	360:376	the concentration of media components using Response surface methodology	360:431	The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology.
31067487	1	59	theme	actinobacterial	250:264	arg1	GD5					301:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	the actinobacterial probiont Streptomyces griseorubens GD5	246:303	In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5.
30840139	9	0	theme	process	1632:1638	arg1	cytocompatibility					1596:1612	the cytocompatibility	1592:1612	the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation	1592:1744	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	3	1	theme	layer-wise	727:736	arg1	printing					738:745	layer-wise printing	727:745	layer-wise printing	727:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	7	2	dep	~790	1493:1496	arg1	to					1490:1491	to	1490:1491	to	1490:1491	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	5	3	theme	methacrylated	1028:1040	arg1	acid					1053:1056	methacrylated hyaluronic acid	1028:1056	methacrylated hyaluronic acid	1028:1056	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	3	4	theme	hydrogels	714:722	arg1	composition					699:709	graded composition	692:709	graded composition of hydrogels by layer-wise printing	692:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	5	5	theme	acetyl	969:974	arg1	GMA					977:979	GMA	977:979	GMA	977:979	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	5	theme	acetyl	969:974	arg1	constant					986:993	constant	986:993	constant	986:993	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	5	theme	acetyl	969:974	arg1	acetyl					969:974	gelatin methacryloyl acetyl	948:974	gelatin methacryloyl acetyl (GMA)	948:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	5	theme	acetyl	969:974	arg1	%					998:998	10%	996:998	10%	996:998	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	5	theme	acetyl	969:974	arg1	amount					938:943	the amount	934:943	the amount of gelatin methacryloyl acetyl (GMA)	934:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	1	6	theme	adequate	274:281	arg1	hydrogels					158:166	Biobased hydrogels	149:166	Biobased hydrogels	149:166	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	1	6	theme	adequate	274:281	arg1	matrices					283:290	adequate matrices	274:290	adequate matrices for cell encapsulation	274:313	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	0	7	theme	swelling	102:109	arg1	control					91:97	independent control	79:97	independent control of swelling and stiffness in printable hydrogels	79:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	7	8	theme	GMA	1362:1364	arg1	DM					1352:1353	the DM	1348:1353	the DM of the GMA	1348:1364	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	6	9	dep	~600	1286:1289	arg1	to					1283:1284	to	1283:1284	to	1283:1284	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	6	10	theme	methacryloylation	1155:1171	arg1	degree					1145:1150	one fixed degree	1135:1150	one fixed degree of methacryloylation (DM)	1135:1176	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	3	11	with	hydrogels	677:685	arg1	composition					699:709	graded composition	692:709	graded composition of hydrogels by layer-wise printing	692:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	6	12	theme	first	1100:1104	arg1	set					1106:1108	the first set	1096:1108	the first set of formulations	1096:1124	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	6	12	theme	first	1100:1104	arg1	formulations					1113:1124	formulations	1113:1124	formulations	1113:1124	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	9	13	theme	photoinitiator	1650:1663	arg1	phenyl-2,4,6-trimethylbenzoylphosphinate					1673:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	10	14	theme	new	1852:1854	arg1	matrix					1856:1861	new matrix	1852:1861	new matrix	1852:1861	Glycosaminoglycan gradient was proved stable for 28 d,encapsulated chondrocytes were viable and produced new matrix.
30840139	3	15	theme	contribution	552:563	arg1	aim					540:542	The aim	536:542	The aim of this contribution	536:563	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	2	16	theme	simple	383:388	arg1	formulations					399:410	simple hydrogel formulations	383:410	simple hydrogel formulations	383:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	2	17	theme	formulations	399:410	arg1	swelling					351:358	swelling	351:358	swelling (EDS)	351:364	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	2	17	theme	formulations	399:410	arg1	EDS					361:363	EDS	361:363	EDS	361:363	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	2	17	theme	formulations	399:410	arg1	stiffness					370:378	stiffness	370:378	stiffness of simple hydrogel formulations	370:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	0	18	theme	stiffness	115:123	arg1	control					91:97	independent control	79:97	independent control of swelling and stiffness in printable hydrogels	79:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	9	19	theme	UVA	1730:1732	arg1	irradiation					1734:1744	layer-wise UVA irradiation	1719:1744	layer-wise UVA irradiation	1719:1744	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	2	20	theme	hydrogel	390:397	arg1	formulations					399:410	simple hydrogel formulations	383:410	simple hydrogel formulations	383:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	6	21	dep	~25	1243:1245	arg1	to					1240:1241	to	1240:1241	to	1240:1241	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	6	22	theme	fixed	1139:1143	arg1	degree					1145:1150	one fixed degree	1135:1150	one fixed degree of methacryloylation (DM)	1135:1176	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	2	23	theme	equilibrium	329:339	arg1	degree					341:346	the equilibrium degree	325:346	the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations	325:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	1	24	theme	extracellular	199:211	arg1	matrix					213:218	native extracellular matrix	192:218	native extracellular matrix due to their high water content	192:250	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	0	25	theme	Advanced	0:7	arg1	formulation					9:19	Advanced formulation	0:19	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.	0:147	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	7	26	theme	concentration	1401:1413	arg1	increase					1381:1388	increase	1381:1388	increase of polymer concentration	1381:1413	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	5	27	theme	gelatin	948:954	arg1	GMA					977:979	GMA	977:979	GMA	977:979	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	27	theme	gelatin	948:954	arg1	acetyl					969:974	gelatin methacryloyl acetyl	948:974	gelatin methacryloyl acetyl (GMA)	948:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	7	28	theme	both	1443:1446	arg1	increase					1431:1438	an increase	1428:1438	an increase of both, G' from ~11 to ~18 kPa	1428:1470	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	7	28	theme	both	1443:1446	arg1	EDS					1476:1478	EDS	1476:1478	EDS	1476:1478	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	0	29	theme	methacryl-	24:33	arg1	biomolecules					55:66	methacryl- and acetyl-modified biomolecules	24:66	methacryl- and acetyl-modified biomolecules	24:66	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	1	30	theme	cell	296:299	arg1	encapsulation					301:313	cell encapsulation	296:313	cell encapsulation	296:313	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	4	31	theme	formulations	776:787	arg1	formulations					776:787	formulations	776:787	formulations	776:787	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	4	31	theme	formulations	776:787	arg1	sets					768:771	two sets	764:771	two sets	764:771	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	6	32	theme	storage	1200:1206	arg1	G					1217:1217	G'	1217:1218	G'	1217:1218	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	6	32	theme	storage	1200:1206	arg1	modulus					1208:1214	the storage modulus	1196:1214	the storage modulus (G')	1196:1219	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	1	33	theme	due	220:222	arg1	matrix					213:218	native extracellular matrix	192:218	native extracellular matrix due to their high water content	192:250	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	2	34	theme	Increasing	436:445	arg1	concentration					455:467	Increasing polymer concentration	436:467	Increasing polymer concentration	436:467	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	0	35	theme	printable	128:136	arg1	hydrogels					138:146	printable hydrogels	128:146	printable hydrogels	128:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	5	36	theme	acid	1053:1056	arg1	proportion					1014:1023	the proportion	1010:1023	the proportion of methacrylated hyaluronic acid and chondroitin sulfate	1010:1080	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	3	37	theme	standard	586:593	arg1	correlation					595:605	this standard correlation	581:605	this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing	581:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	7	38	theme	formulations	1314:1325	arg1	formulations					1314:1325	formulations	1314:1325	formulations	1314:1325	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	7	38	theme	formulations	1314:1325	arg1	set					1307:1309	the second set	1296:1309	the second set of formulations	1296:1325	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	0	39	theme	acetyl-modified	39:53	arg1	biomolecules					55:66	methacryl- and acetyl-modified biomolecules	24:66	methacryl- and acetyl-modified biomolecules	24:66	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	8	40	theme	glycosaminoglycan-graded	1547:1570	arg1	hydrogel					1572:1579	a glycosaminoglycan-graded hydrogel	1545:1579	a glycosaminoglycan-graded hydrogel	1545:1579	By dispensing these compositions, we created a glycosaminoglycan-graded hydrogel.
30840139	0	41	from	control	91:97	arg1	hydrogels					138:146	printable hydrogels	128:146	printable hydrogels	128:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	4	42	theme	total	854:858	arg1	concentration					871:883	total biopolymer concentration	854:883	total biopolymer concentration (10.6%, 11.5%, 13.0%)	854:905	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	6	43	used	used	1182:1185	arg2	GMA					1126:1128	GMA	1126:1128	GMA with one fixed degree of methacryloylation (DM)	1126:1176	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	5	44	dep	constant	986:993	arg1	acetyl					969:974	gelatin methacryloyl acetyl	948:974	gelatin methacryloyl acetyl (GMA)	948:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	44	dep	constant	986:993	arg1	constant					986:993	constant	986:993	constant	986:993	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	44	dep	constant	986:993	arg1	%					998:998	10%	996:998	10%	996:998	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	44	dep	constant	986:993	arg1	amount					938:943	the amount	934:943	the amount of gelatin methacryloyl acetyl (GMA)	934:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	7	45	dep	~18	1464:1466	arg1	to					1461:1462	to	1461:1462	to	1461:1462	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	9	46	theme	irradiation	1734:1744	arg1	cytocompatibility					1596:1612	the cytocompatibility	1592:1612	the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation	1592:1744	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	5	47	theme	chondroitin	1062:1072	arg1	sulfate					1074:1080	chondroitin sulfate	1062:1080	chondroitin sulfate	1062:1080	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	2	48	theme	polymer	447:453	arg1	concentration					455:467	Increasing polymer concentration	436:467	Increasing polymer concentration	436:467	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	1	49	theme	high	233:236	arg1	content					244:250	their high water content	227:250	their high water content	227:250	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	6	50	with	GMA	1126:1128	arg1	degree					1145:1150	one fixed degree	1135:1150	one fixed degree of methacryloylation (DM)	1135:1176	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	5	51	theme	sulfate	1074:1080	arg1	proportion					1014:1023	the proportion	1010:1023	the proportion of methacrylated hyaluronic acid and chondroitin sulfate	1010:1080	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	3	52	theme	polymer	615:621	arg1	content					623:629	polymer content	615:629	polymer content	615:629	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	6	53	theme	formulations	1113:1124	arg1	set					1106:1108	the first set	1096:1108	the first set of formulations	1096:1124	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	6	53	theme	formulations	1113:1124	arg1	formulations					1113:1124	formulations	1113:1124	formulations	1113:1124	In the first set of formulations GMA with one fixed degree of methacryloylation (DM) was used, whereby the storage modulus (G') increased from ~10 to ~25 kPa and the EDS decreased from ~700 to ~600%.
30840139	0	54	theme	biomolecules	55:66	arg1	formulation					9:19	Advanced formulation	0:19	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.	0:147	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	10	55	theme	Glycosaminoglycan	1747:1763	arg1	gradient					1765:1772	Glycosaminoglycan gradient	1747:1772	Glycosaminoglycan gradient	1747:1772	Glycosaminoglycan gradient was proved stable for 28 d,encapsulated chondrocytes were viable and produced new matrix.
30840139	3	56	theme	graded	692:697	arg1	composition					699:709	graded composition	692:709	graded composition of hydrogels by layer-wise printing	692:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	4	57	theme	biopolymer	860:869	arg1	concentration					871:883	total biopolymer concentration	854:883	total biopolymer concentration (10.6%, 11.5%, 13.0%)	854:905	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	10	58	theme	encapsulated	1801:1812	arg1	chondrocytes					1814:1825	encapsulated chondrocytes	1801:1825	encapsulated chondrocytes	1801:1825	Glycosaminoglycan gradient was proved stable for 28 d,encapsulated chondrocytes were viable and produced new matrix.
30840139	2	59	theme	swelling	351:358	arg1	degree					341:346	the equilibrium degree	325:346	the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations	325:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	7	60	theme	polymer	1393:1399	arg1	concentration					1401:1413	polymer concentration	1393:1413	polymer concentration	1393:1413	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	4	61	theme	different	815:823	arg1	compositions					825:836	three different compositions	809:836	three different compositions	809:836	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	7	62	theme	second	1300:1305	arg1	formulations					1314:1325	formulations	1314:1325	formulations	1314:1325	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	7	62	theme	second	1300:1305	arg1	set					1307:1309	the second set	1296:1309	the second set of formulations	1296:1325	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	5	63	theme	methacryloyl	956:967	arg1	GMA					977:979	GMA	977:979	GMA	977:979	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	5	63	theme	methacryloyl	956:967	arg1	acetyl					969:974	gelatin methacryloyl acetyl	948:974	gelatin methacryloyl acetyl (GMA)	948:980	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	1	64	theme	water	238:242	arg1	content					244:250	their high water content	227:250	their high water content	227:250	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	7	65	from	kPa	1468:1470	arg1	increase					1431:1438	an increase	1428:1438	an increase of both, G' from ~11 to ~18 kPa	1428:1470	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	7	65	from	kPa	1468:1470	arg1	EDS					1476:1478	EDS	1476:1478	EDS	1476:1478	In the second set of formulations we gradually lowered the DM of the GMA in parallel to increase of polymer concentration and achieved an increase of both, G' from ~11 to ~18 kPa and EDS from ~690 to ~790%.
30840139	3	66	theme	hydrogels	677:685	arg1	assembly					665:672	the assembly	661:672	this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing	581:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	3	66	theme	hydrogels	677:685	arg1	correlation					595:605	this standard correlation	581:605	this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing	581:745	The aim of this contribution was to decouple this standard correlation between polymer content, stiffness and EDS as well as the assembly of hydrogels with graded composition of hydrogels by layer-wise printing.
30840139	9	67	theme	lithium	1665:1671	arg1	phenyl-2,4,6-trimethylbenzoylphosphinate					1673:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	1	68	theme	Biobased	149:156	arg1	hydrogels					158:166	Biobased hydrogels	149:166	Biobased hydrogels	149:166	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	1	68	theme	Biobased	149:156	arg1	matrices					283:290	adequate matrices	274:290	adequate matrices for cell encapsulation	274:313	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	0	69	theme	independent	79:89	arg1	control					91:97	independent control	79:97	independent control of swelling and stiffness in printable hydrogels	79:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	5	70	theme	hyaluronic	1042:1051	arg1	acid					1053:1056	methacrylated hyaluronic acid	1028:1056	methacrylated hyaluronic acid	1028:1056	Within these compositions the amount of gelatin methacryloyl acetyl (GMA) was constant (10%), whereas the proportion of methacrylated hyaluronic acid and chondroitin sulfate increased.
30840139	0	71	dep	formulation	9:19	arg1	achieve					71:77	achieve	71:77	to achieve independent control of swelling and stiffness in printable hydrogels	68:146	Advanced formulation of methacryl- and acetyl-modified biomolecules to achieve independent control of swelling and stiffness in printable hydrogels.
30840139	4	72	dep	concentration	871:883	arg1	%					890:890	10.6%	886:890	10.6%	886:890	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	4	72	dep	concentration	871:883	arg1	%					897:897	11.5%	893:897	11.5%	893:897	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	4	72	dep	concentration	871:883	arg1	%					904:904	13.0%	900:904	13.0%	900:904	We investigated two sets of formulations, which consisted of three different compositions with increasing total biopolymer concentration (10.6%, 11.5%, 13.0%).
30840139	2	73	theme	stiffness	370:378	arg1	degree					341:346	the equilibrium degree	325:346	the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations	325:410	However, the equilibrium degree of swelling (EDS) and stiffness of simple hydrogel formulations are typically confined: Increasing polymer concentration results in increasing stiffness and simultaneously decreasing EDS.
30840139	1	74	theme	native	192:197	arg1	matrix					213:218	native extracellular matrix	192:218	native extracellular matrix due to their high water content	192:250	Biobased hydrogels are considered to mimic native extracellular matrix due to their high water content and are considered as adequate matrices for cell encapsulation.
30840139	9	75	theme	dispensing	1621:1630	arg1	process					1632:1638	the dispensing process	1617:1638	the dispensing process	1617:1638	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	9	76	theme	layer-wise	1719:1728	arg1	irradiation					1734:1744	layer-wise UVA irradiation	1719:1744	layer-wise UVA irradiation	1719:1744	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	9	77	theme	phenyl-2,4,6-trimethylbenzoylphosphinate	1673:1712	arg1	cytocompatibility					1596:1612	the cytocompatibility	1592:1612	the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation	1592:1744	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
30840139	9	78	theme	used	1645:1648	arg1	phenyl-2,4,6-trimethylbenzoylphosphinate					1673:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate	1641:1712	We proved the cytocompatibility of the dispensing process, the used photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate, and layer-wise UVA irradiation.
29579981	0	0	theme	Carignan	80:87	arg1	variety					95:101	Carignan grape variety	80:101	Carignan grape variety	80:101	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	0	1	from	effects	9:15	arg1	profiles					57:64	the polyphenolic and polysaccharidic profiles	20:64	the polyphenolic and polysaccharidic profiles	20:64	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	3	2	theme	several	488:494	arg1	fractions					496:504	several fractions	488:504	several fractions	488:504	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	0	3	theme	variety	95:101	arg1	Location					0:7	Location effects on the polyphenolic and polysaccharidic profiles and colour	0:75	Location	0:7	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	0	3	theme	variety	95:101	arg1	colour					70:75	colour	70:75	colour	70:75	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	3	4	theme	polysaccharides	531:545	arg1	composition					369:379	polyphenolic composition	356:379	polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins)	356:482	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	4	theme	polysaccharides	531:545	arg1	fractions					496:504	several fractions	488:504	several fractions	488:504	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	4	5	theme	locations	750:758	arg1	fingerprints					720:731	fingerprints	720:731	fingerprints of the different locations of the Maule valley wines	720:784	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	0	6	theme	grape	89:93	arg1	variety					95:101	Carignan grape variety	80:101	Carignan grape variety	80:101	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	3	7	dep	composition	369:379	arg1	derivatives					418:428	hydroxycinnamic acid derivatives	397:428	hydroxycinnamic acid derivatives	397:428	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	7	dep	composition	369:379	arg1	flavonols					456:464	flavonols	456:464	flavonols	456:464	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	7	dep	composition	369:379	arg1	anthocyanins					470:481	anthocyanins	470:481	anthocyanins	470:481	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	7	dep	composition	369:379	arg1	acids					390:394	benzoic acids	382:394	benzoic acids	382:394	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	7	dep	composition	369:379	arg1	stilbenes					431:439	stilbenes	431:439	stilbenes	431:439	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	7	dep	composition	369:379	arg1	flavan-3-ols					442:453	flavan-3-ols	442:453	flavan-3-ols	442:453	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	8	theme	cis-resveratrol-glucoside	909:933	arg1	content					851:857	higher content	844:857	higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal)	844:958	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	9	theme	Andes	824:828	arg1	Mountains					830:838	the Andes Mountains	820:838	the Andes Mountains	820:838	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	3	10	theme	proanthocyanidins	509:525	arg1	composition					369:379	polyphenolic composition	356:379	polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins)	356:482	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	3	10	theme	proanthocyanidins	509:525	arg1	fractions					496:504	several fractions	488:504	several fractions	488:504	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	4	11	theme	production	669:678	arg1	year					680:683	the production year	665:683	the production year	665:683	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	3	12	theme	benzoic	382:388	arg1	acids					390:394	benzoic acids	382:394	benzoic acids	382:394	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	13	contain	had	840:842	arg2	content					851:857	higher content	844:857	higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal)	844:958	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	13	contain	had	840:842	arg1	wines					793:797	wines	793:797	wines from zones closer to the Andes Mountains	793:838	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	4	14	theme	valley	773:778	arg1	wines					780:784	the Maule valley wines	763:784	the Maule valley wines	763:784	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	3	15	theme	polyphenolic	356:367	arg1	composition					369:379	polyphenolic composition	356:379	polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins)	356:482	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	16	from	zones	804:808	arg1	wines					793:797	wines	793:797	wines from zones closer to the Andes Mountains	793:838	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	4	17	theme	different	740:748	arg1	locations					750:758	the different locations	736:758	the different locations of the Maule valley wines	736:784	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	3	18	theme	hydroxycinnamic	397:411	arg1	derivatives					418:428	hydroxycinnamic acid derivatives	397:428	hydroxycinnamic acid derivatives	397:428	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	19	theme	higher	844:849	arg1	content					851:857	higher content	844:857	higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal)	844:958	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	3	20	theme	acid	413:416	arg1	derivatives					418:428	hydroxycinnamic acid derivatives	397:428	hydroxycinnamic acid derivatives	397:428	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	21	theme	closer	810:815	arg1	zones					804:808	zones	804:808	zones closer to the Andes Mountains	804:838	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	0	22	theme	Chilean	118:124	arg1	region					132:137	the Chilean Maule region	114:137	the Chilean Maule region	114:137	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	5	23	dep	B3	874:875	arg1	Melozal					951:957	Melozal	951:957	Melozal	951:957	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	23	dep	B3	874:875	arg1	Loncomilla					936:945	Loncomilla	936:945	Loncomilla	936:945	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	1	24	theme	chemical	173:180	arg1	characterization					182:197	chemical characterization	173:197	chemical characterization	173:197	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	4	25	theme	wines	780:784	arg1	locations					750:758	the different locations	736:758	the different locations of the Maule valley wines	736:784	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	5	26	theme	colorimetric	1047:1058	arg1	terms					1060:1064	chemical and colorimetric terms	1034:1064	chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule)	1034:1105	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	26	theme	colorimetric	1047:1058	arg1	Sauzal					1078:1083	Sauzal	1078:1083	Sauzal	1078:1083	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	26	theme	colorimetric	1047:1058	arg1	Maule					1100:1104	Maule	1100:1104	Maule	1100:1104	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	2	27	theme	production	274:283	arg1	years					285:289	two production years	270:289	two production years	270:289	Carignan red wines from six locations and two production years of the Chilean Maule valley.
29579981	1	28	theme	characterization	182:197	arg1	study					164:168	a study	162:168	a study of chemical characterization and colour parameters of cv	162:225	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	5	29	theme	polysaccharides	889:903	arg1	content					851:857	higher content	844:857	higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal)	844:958	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	30	theme	procyanidin	862:872	arg1	Caliboro					878:885	Caliboro	878:885	Caliboro	878:885	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	30	theme	procyanidin	862:872	arg1	B3					874:875	procyanidin B3	862:875	procyanidin B3 (Caliboro)	862:886	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	0	31	theme	polyphenolic	24:35	arg1	profiles					57:64	the polyphenolic and polysaccharidic profiles	20:64	the polyphenolic and polysaccharidic profiles	20:64	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	0	32	theme	Maule	126:130	arg1	region					132:137	the Chilean Maule region	114:137	the Chilean Maule region	114:137	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	4	33	theme	anthocyanins	625:636	arg1	content					614:620	significantly (p < 0.05) different content	579:620	significantly (p < 0.05) different content of anthocyanins	579:636	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	1	34	theme	colour	203:208	arg1	parameters					210:219	colour parameters	203:219	colour parameters	203:219	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	4	35	dep	significantly	579:591	arg1	p < 0.05					594:601	p < 0.05	594:601	p < 0.05	594:601	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	0	36	theme	polysaccharidic	41:55	arg1	profiles					57:64	the polyphenolic and polysaccharidic profiles	20:64	the polyphenolic and polysaccharidic profiles	20:64	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	3	37	theme	chemical	324:331	arg1	study					333:337	The chemical study	320:337	The chemical study	320:337	The chemical study was performed on polyphenolic composition (benzoic acids, hydroxycinnamic acid derivatives, stilbenes, flavan-3-ols, flavonols and anthocyanins) and several fractions of proanthocyanidins and polysaccharides.
29579981	5	38	theme	unifying	1015:1022	arg1	effect					1024:1029	a unifying effect	1013:1029	a unifying effect	1013:1029	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	2	39	theme	valley	312:317	arg1	years					285:289	two production years	270:289	two production years	270:289	Carignan red wines from six locations and two production years of the Chilean Maule valley.
29579981	2	39	theme	valley	312:317	arg1	locations					256:264	six locations	252:264	six locations	252:264	Carignan red wines from six locations and two production years of the Chilean Maule valley.
29579981	5	40	theme	Pacific	990:996	arg1	Ocean					998:1002	the Pacific Ocean	986:1002	the Pacific Ocean	986:1002	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	2	41	theme	Maule	306:310	arg1	valley					312:317	the Chilean Maule valley	294:317	the Chilean Maule valley	294:317	Carignan red wines from six locations and two production years of the Chilean Maule valley.
29579981	1	42	theme	parameters	210:219	arg1	study					164:168	a study	162:168	a study of chemical characterization and colour parameters of cv	162:225	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	5	43	theme	chemical	1034:1041	arg1	terms					1060:1064	chemical and colorimetric terms	1034:1064	chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule)	1034:1105	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	43	theme	chemical	1034:1041	arg1	Sauzal					1078:1083	Sauzal	1078:1083	Sauzal	1078:1083	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	43	theme	chemical	1034:1041	arg1	Maule					1100:1104	Maule	1100:1104	Maule	1100:1104	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	4	44	theme	Maule	767:771	arg1	wines					780:784	the Maule valley wines	763:784	the Maule valley wines	763:784	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	2	45	theme	Chilean	298:304	arg1	valley					312:317	the Chilean Maule valley	294:317	the Chilean Maule valley	294:317	Carignan red wines from six locations and two production years of the Chilean Maule valley.
29579981	4	46	theme	different	604:612	arg1	content					614:620	significantly (p < 0.05) different content	579:620	significantly (p < 0.05) different content of anthocyanins	579:636	Results revealed that although significantly (p < 0.05) different content of anthocyanins were observed according to the production year, it could be possible to establish fingerprints of the different locations of the Maule valley wines.
29579981	5	47	theme	B3	874:875	arg1	content					851:857	higher content	844:857	higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal)	844:958	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	1	48	theme	cv	224:225	arg1	characterization					182:197	chemical characterization	173:197	chemical characterization	173:197	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	1	48	theme	cv	224:225	arg1	parameters					210:219	colour parameters	203:219	colour parameters	203:219	This paper reports on a study of chemical characterization and colour parameters of cv.
29579981	0	49	dep	Location	0:7	arg1	effects					9:15	effects	9:15	effects on the polyphenolic and polysaccharidic profiles	9:64	Location effects on the polyphenolic and polysaccharidic profiles and colour of Carignan grape variety wines from the Chilean Maule region.
29579981	5	50	dep	terms	1060:1064	arg1	Maule					1100:1104	Maule	1100:1104	Maule	1100:1104	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	50	dep	terms	1060:1064	arg1	Sauzal					1078:1083	Sauzal	1078:1083	Sauzal	1078:1083	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29579981	5	50	dep	terms	1060:1064	arg1	terms					1060:1064	chemical and colorimetric terms	1034:1064	chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule)	1034:1105	Thus, wines from zones closer to the Andes Mountains had higher content of procyanidin B3 (Caliboro), polysaccharides and cis-resveratrol-glucoside (Loncomilla and Melozal), whereas the proximity to the Pacific Ocean provoked a unifying effect in chemical and colorimetric terms (Cauquenes, Sauzal and Huerta del Maule).
29501428	1	0	theme	mode	195:198	arg1	CZE-MS					219:224	CZE-MS	219:224	CZE-MS	219:224	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	0	theme	mode	195:198	arg1	spectrometry					205:216	negative ion mode mass spectrometry	182:216	negative ion mode mass spectrometry (CZE-MS)	182:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	2	1	with	modification	331:342	arg1	reagents					415:422	neutral or cationic reagents	395:422	neutral or cationic reagents	395:422	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	0	2	theme	electrophoresis-mass	88:107	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	1	3	theme	mass	200:203	arg1	CZE-MS					219:224	CZE-MS	219:224	CZE-MS	219:224	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	3	theme	mass	200:203	arg1	spectrometry					205:216	negative ion mode mass spectrometry	182:216	negative ion mode mass spectrometry (CZE-MS)	182:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	6	4	theme	sulfate	1019:1025	arg1	oligosaccharides					1027:1042	heparin/heparan sulfate oligosaccharides	1003:1042	heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12	1003:1083	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	2	5	theme	separation	369:378	arg1	capillary					380:388	the separation capillary	365:388	the separation capillary	365:388	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	5	6	theme	epimeric	917:924	arg1	variation					926:934	epimeric variation	917:934	epimeric variation	917:934	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	2	7	theme	Covalent	322:329	arg1	modification					331:342	Covalent modification	322:342	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents	322:422	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	6	8	theme	heparin/heparan	1003:1017	arg1	sulfate					1019:1025	heparin/heparan sulfate	1003:1025	heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12	1003:1083	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	2	9	theme	capillary	380:388	arg1	wall					357:360	the inner wall	347:360	the inner wall of the separation capillary	347:388	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	3	10	from	dp4	680:682	arg1	analysis					637:644	the analysis	633:644	the analysis of sulfated oligosaccharides from dp4 to dp12	633:690	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	3	10	from	dp4	680:682	arg1	oligosaccharides					658:673	sulfated oligosaccharides	649:673	sulfated oligosaccharides from dp4 to dp12	649:690	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	5	11	theme	approach	833:840	arg1	capability					814:823	the capability	810:823	the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid	810:953	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	3	12	theme	flow	566:569	arg1	interface					571:579	a sheath flow interface	557:579	a sheath flow interface	557:579	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	3	13	from	analysis	637:644	arg1	dp4					680:682	dp4	680:682	dp4 to dp12	680:690	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	6	14	theme	oligosaccharides	1027:1042	arg1	mixture					992:998	a complex mixture	982:998	a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12	982:1083	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	6	15	theme	chain	1055:1059	arg1	length					1061:1066	chain length	1055:1066	chain length	1055:1066	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	6	16	theme	molecular	1102:1110	arg1	compositions					1112:1123	more than 80 molecular compositions	1089:1123	more than 80 molecular compositions	1089:1123	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	2	17	theme	reproducible	476:487	arg1	separations					489:499	reproducible separations	476:499	reproducible separations	476:499	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	3	18	theme	sheath	559:564	arg1	interface					571:579	a sheath flow interface	557:579	a sheath flow interface	557:579	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	3	19	theme	reliable	594:601	arg1	method					603:608	a rapid and reliable method	582:608	a rapid and reliable method	582:608	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	2	20	theme	cationic	406:413	arg1	reagents					415:422	neutral or cationic reagents	395:422	neutral or cationic reagents	395:422	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	0	21	theme	Heparin/heparan	0:14	arg1	sulfate					16:22	Heparin/heparan sulfate	0:22	Heparin/heparan sulfate	0:22	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	1	22	theme	Reverse	123:129	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	22	theme	Reverse	123:129	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	4	23	theme	Several	693:699	arg1	mixtures					711:718	Several different mixtures	693:718	Several different mixtures	693:718	Several different mixtures have been separated and detected by mass spectrometry.
29501428	1	24	theme	glycosaminoglycan	294:310	arg1	mixtures					312:319	glycosaminoglycan mixtures	294:319	glycosaminoglycan mixtures	294:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	25	theme	polarity	131:138	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	25	theme	polarity	131:138	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	2	26	theme	durable	446:452	arg1	surface					454:460	a stable and durable surface	433:460	a stable and durable surface that provides reproducible separations	433:499	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	5	27	theme	subtle	853:858	arg1	variation					926:934	epimeric variation	917:934	epimeric variation	917:934	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	5	27	theme	subtle	853:858	arg1	differences					860:870	subtle differences	853:870	subtle differences	853:870	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	5	27	theme	subtle	853:858	arg1	position					904:911	sulfation position	894:911	sulfation position	894:911	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	1	28	theme	mixtures	312:319	arg1	analysis					282:289	the analysis	278:289	the analysis of glycosaminoglycan mixtures	278:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	29	theme	capillary	140:148	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	29	theme	capillary	140:148	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	2	30	theme	neutral	395:401	arg1	reagents					415:422	neutral or cationic reagents	395:422	neutral or cationic reagents	395:422	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	0	31	theme	modified	47:54	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	4	32	theme	different	701:709	arg1	mixtures					711:718	Several different mixtures	693:718	Several different mixtures	693:718	Several different mixtures have been separated and detected by mass spectrometry.
29501428	6	33	theme	mass	1153:1156	arg1	measurement					1158:1168	accurate mass measurement	1144:1168	accurate mass measurement	1144:1168	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	2	34	theme	stable	435:440	arg1	surface					454:460	a stable and durable surface	433:460	a stable and durable surface that provides reproducible separations	433:499	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	5	35	theme	sulfation	894:902	arg1	position					904:911	sulfation position	894:911	sulfation position	894:911	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	3	36	theme	oligosaccharides	658:673	arg1	analysis					637:644	the analysis	633:644	the analysis of sulfated oligosaccharides from dp4 to dp12	633:690	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	5	37	theme	uronic	943:948	arg1	acid					950:953	the uronic acid	939:953	the uronic acid	939:953	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	0	38	theme	polarity	64:71	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	6	39	theme	complex	984:990	arg1	mixture					992:998	a complex mixture	982:998	a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12	982:1083	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	6	40	theme	accurate	1144:1151	arg1	measurement					1158:1168	accurate mass measurement	1144:1168	accurate mass measurement	1144:1168	The system was applied to a complex mixture of heparin/heparan sulfate oligosaccharides varying in chain length from dp3 to dp12 and more than 80 molecular compositions were identified by accurate mass measurement.
29501428	1	41	theme	zone	150:153	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	41	theme	zone	150:153	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	0	42	theme	reverse	56:62	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	5	43	from	differences	860:870	arg1	structure					875:883	structure	875:883	structure	875:883	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	3	44	theme	cation-coated	529:541	arg1	capillary					543:551	a cation-coated capillary	527:551	a cation-coated capillary	527:551	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	4	45	theme	mass	756:759	arg1	spectrometry					761:772	mass spectrometry	756:772	mass spectrometry	756:772	Several different mixtures have been separated and detected by mass spectrometry.
29501428	2	46	theme	wall	357:360	arg1	modification					331:342	Covalent modification	322:342	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents	322:422	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	3	47	theme	rapid	584:588	arg1	method					603:608	a rapid and reliable method	582:608	a rapid and reliable method	582:608	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	5	48	theme	acid	950:953	arg1	variation					926:934	epimeric variation	917:934	epimeric variation	917:934	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	5	48	theme	acid	950:953	arg1	position					904:911	sulfation position	894:911	sulfation position	894:911	The mixtures were selected to test the capability of this approach to resolve subtle differences in structure, such as sulfation position and epimeric variation of the uronic acid.
29501428	3	49	theme	sulfated	649:656	arg1	oligosaccharides					658:673	sulfated oligosaccharides	649:673	sulfated oligosaccharides from dp4 to dp12	649:690	By combining CZE-MS with a cation-coated capillary and a sheath flow interface, a rapid and reliable method has been developed for the analysis of sulfated oligosaccharides from dp4 to dp12.
29501428	2	50	theme	inner	351:355	arg1	wall					357:360	the inner wall	347:360	the inner wall of the separation capillary	347:388	Covalent modification of the inner wall of the separation capillary with neutral or cationic reagents produces a stable and durable surface that provides reproducible separations.
29501428	1	51	theme	effective	245:253	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	51	theme	effective	245:253	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	0	52	theme	zone	83:86	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	1	53	theme	negative	182:189	arg1	CZE-MS					219:224	CZE-MS	219:224	CZE-MS	219:224	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	53	theme	negative	182:189	arg1	spectrometry					205:216	negative ion mode mass spectrometry	182:216	negative ion mode mass spectrometry (CZE-MS)	182:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	0	54	theme	capillary	73:81	arg1	spectrometry					109:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry	36:120	Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.
29501428	1	55	theme	ion	191:193	arg1	CZE-MS					219:224	CZE-MS	219:224	CZE-MS	219:224	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	55	theme	ion	191:193	arg1	spectrometry					205:216	negative ion mode mass spectrometry	182:216	negative ion mode mass spectrometry (CZE-MS)	182:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	56	theme	sensitive	259:267	arg1	electrophoresis					155:169	Reverse polarity capillary zone electrophoresis	123:169	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS)	123:225	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
29501428	1	56	theme	sensitive	259:267	arg1	tool					269:272	an effective and sensitive tool	242:272	an effective and sensitive tool for the analysis of glycosaminoglycan mixtures	242:319	Reverse polarity capillary zone electrophoresis coupled to negative ion mode mass spectrometry (CZE-MS) is shown to be an effective and sensitive tool for the analysis of glycosaminoglycan mixtures.
30926505	10	0	theme	regeneration	1501:1512	arg1	view					1428:1431	view	1428:1431	view of easy preparation and recovery, effectiveness adsorption and good regeneration	1428:1512	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	9	1	theme	starting	1378:1385	arg1	ones					1387:1390	starting ones	1378:1390	starting ones	1378:1390	Furthermore, milligels displayed good reusability after five adsorption/desorption cycles and with an augment in their removal ability compared to starting ones, reaching 714 mg g-1 for R-C/D1.
30926505	0	2	theme	efficient	92:100	arg1	adsorbents					102:111	reusable and efficient adsorbents	79:111	reusable and efficient adsorbents of cationic dye methylene blue	79:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	0	2	theme	efficient	92:100	arg1	beads					70:74	sulfate composite beads	52:74	sulfate composite beads	52:74	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	10	3	theme	good	1496:1499	arg1	regeneration					1501:1512	good regeneration	1496:1512	good regeneration	1496:1512	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	10	4	theme	preparation	1441:1451	arg1	view					1428:1431	view	1428:1431	view of easy preparation and recovery, effectiveness adsorption and good regeneration	1428:1512	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	9	5	theme	adsorption/desorption	1292:1312	arg1	cycles					1314:1319	five adsorption/desorption cycles	1287:1319	five adsorption/desorption cycles	1287:1319	Furthermore, milligels displayed good reusability after five adsorption/desorption cycles and with an augment in their removal ability compared to starting ones, reaching 714 mg g-1 for R-C/D1.
30926505	9	6	dep	an	1330:1331	arg1	augment					1333:1339	augment	1333:1339	augment in their removal ability compared to starting ones	1333:1390	Furthermore, milligels displayed good reusability after five adsorption/desorption cycles and with an augment in their removal ability compared to starting ones, reaching 714 mg g-1 for R-C/D1.
30926505	0	7	theme	cationic	116:123	arg1	dye					125:127	cationic dye	116:127	cationic dye methylene blue	116:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	9	8	theme	removal	1350:1356	arg1	ability					1358:1364	their removal ability	1344:1364	their removal ability	1344:1364	Furthermore, milligels displayed good reusability after five adsorption/desorption cycles and with an augment in their removal ability compared to starting ones, reaching 714 mg g-1 for R-C/D1.
30926505	6	9	theme	adsorption	939:948	arg1	studies					950:956	isotherm adsorption studies	930:956	isotherm adsorption studies	930:956	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	10	10	theme	easy	1436:1439	arg1	preparation					1441:1451	preparation	1441:1451	preparation	1441:1451	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	4	11	theme	DS	615:616	arg1	effect					605:610	The effect	601:610	The effect of DS on adsorption of composites for cationic dye methylene blue (MB)	601:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	10	12	theme	adsorption	1481:1490	arg1	view					1428:1431	view	1428:1431	view of easy preparation and recovery, effectiveness adsorption and good regeneration	1428:1512	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	4	13	theme	time	755:758	arg1	contact					760:766	time contact	755:766	time contact	755:766	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	3	14	theme	porous	504:509	arg1	structure					511:519	porous structure	504:519	porous structure	504:519	The composites exhibited porous structure and enhance in swelling properties with enriching DS as well as pH-sensitivity.
30926505	4	15	from	effect	605:610	arg1	adsorption					621:630	adsorption	621:630	adsorption of composites for cationic dye methylene blue (MB)	621:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	1	16	theme	pore-forming	372:383	arg1	template					385:392	pore-forming template	372:392	pore-forming template	372:392	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	8	17	theme	endothermic	1203:1213	arg1	nature					1223:1228	spontaneous and endothermic process nature	1187:1228	spontaneous and endothermic process nature	1187:1228	Thermodynamic study revealed spontaneous and endothermic process nature.
30926505	5	18	theme	adsorption	831:840	arg1	performances					842:853	adsorption performances	831:853	adsorption performances	831:853	The results revealed that adsorption performances were remarkably improved by increasing DS content into beads.
30926505	4	19	theme	dye	769:771	arg1	concentration					773:785	dye concentration	769:785	dye concentration	769:785	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	1	20	theme	crosslinked-carboxymethyl	192:216	arg1	CMC					229:231	CMC	229:231	CMC	229:231	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	20	theme	crosslinked-carboxymethyl	192:216	arg1	cellulose					218:226	crosslinked-carboxymethyl cellulose	192:226	crosslinked-carboxymethyl cellulose (CMC)	192:232	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	0	21	theme	dye	125:127	arg1	adsorbents					102:111	reusable and efficient adsorbents	79:111	reusable and efficient adsorbents of cationic dye methylene blue	79:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	0	21	theme	dye	125:127	arg1	beads					70:74	sulfate composite beads	52:74	sulfate composite beads	52:74	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	10	22	theme	effectiveness	1467:1479	arg1	adsorption					1481:1490	effectiveness adsorption	1467:1490	effectiveness adsorption	1467:1490	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	8	23	theme	process	1215:1221	arg1	nature					1223:1228	spontaneous and endothermic process nature	1187:1228	spontaneous and endothermic process nature	1187:1228	Thermodynamic study revealed spontaneous and endothermic process nature.
30926505	2	24	theme	composites	409:418	arg1	C/Dx					420:423	The milligels composites C/Dx	395:423	The milligels composites C/Dx	395:423	The milligels composites C/Dx were characterized by FTIR, SEM/EDX and TGA analyses.
30926505	0	25	theme	porous	13:18	arg1	carboxymethyl					20:32	Eco-friendly porous carboxymethyl	0:32	Eco-friendly porous carboxymethyl	0:32	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	8	26	theme	Thermodynamic	1158:1170	arg1	study					1172:1176	Thermodynamic study	1158:1176	Thermodynamic study	1158:1176	Thermodynamic study revealed spontaneous and endothermic process nature.
30926505	4	27	theme	adsorbent	737:745	arg1	dosage					747:752	adsorbent dosage	737:752	adsorbent dosage	737:752	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	10	28	theme	wastewater	1573:1582	arg1	treatment					1584:1592	wastewater treatment	1573:1592	wastewater treatment	1573:1592	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	2	29	theme	milligels	399:407	arg1	composites					409:418	milligels composites	399:418	The milligels composites C/Dx	395:423	The milligels composites C/Dx were characterized by FTIR, SEM/EDX and TGA analyses.
30926505	0	30	theme	Eco-friendly	0:11	arg1	carboxymethyl					20:32	Eco-friendly porous carboxymethyl	0:32	Eco-friendly porous carboxymethyl	0:32	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	4	31	dep	investigated	687:698	arg1	dosage					747:752	adsorbent dosage	737:752	adsorbent dosage	737:752	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	31	dep	investigated	687:698	arg1	contact					760:766	time contact	755:766	time contact	755:766	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	31	dep	investigated	687:698	arg1	concentration					773:785	dye concentration	769:785	dye concentration	769:785	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	31	dep	investigated	687:698	arg1	temperature					792:802	temperature	792:802	temperature	792:802	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	31	dep	investigated	687:698	arg1	pH					733:734	pH	733:734	pH	733:734	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	3	32	from	structure	511:519	arg1	properties					545:554	swelling properties	536:554	swelling properties	536:554	The composites exhibited porous structure and enhance in swelling properties with enriching DS as well as pH-sensitivity.
30926505	1	33	theme	ionotropic	303:312	arg1	gelation					314:321	ionotropic gelation	303:321	ionotropic gelation	303:321	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	10	34	theme	low-cost	1550:1557	arg1	composites					1519:1528	the composites	1515:1528	the composites	1515:1528	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	10	34	theme	low-cost	1550:1557	arg1	adsorbents					1559:1568	low-cost adsorbents	1550:1568	low-cost adsorbents in wastewater treatment	1550:1592	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	9	35	theme	good	1264:1267	arg1	reusability					1269:1279	good reusability	1264:1279	good reusability	1264:1279	Furthermore, milligels displayed good reusability after five adsorption/desorption cycles and with an augment in their removal ability compared to starting ones, reaching 714 mg g-1 for R-C/D1.
30926505	4	36	theme	cationic	650:657	arg1	methylene					663:671	cationic dye methylene blue	650:676	cationic dye methylene blue (MB)	650:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	36	theme	cationic	650:657	arg1	MB					679:680	MB	679:680	MB	679:680	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	0	37	theme	blue	139:142	arg1	dye					125:127	cationic dye	116:127	cationic dye methylene blue	116:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	8	38	theme	spontaneous	1187:1197	arg1	nature					1223:1228	spontaneous and endothermic process nature	1187:1228	spontaneous and endothermic process nature	1187:1228	Thermodynamic study revealed spontaneous and endothermic process nature.
30926505	3	39	theme	swelling	536:543	arg1	properties					545:554	swelling properties	536:554	swelling properties	536:554	The composites exhibited porous structure and enhance in swelling properties with enriching DS as well as pH-sensitivity.
30926505	0	40	theme	composite	60:68	arg1	adsorbents					102:111	reusable and efficient adsorbents	79:111	reusable and efficient adsorbents of cationic dye methylene blue	79:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	0	40	theme	composite	60:68	arg1	beads					70:74	sulfate composite beads	52:74	sulfate composite beads	52:74	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	1	41	theme	dextran	238:244	arg1	DS					255:256	DS	255:256	DS	255:256	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	41	theme	dextran	238:244	arg1	sulfate					246:252	dextran sulfate	238:252	dextran sulfate (DS)	238:257	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	0	42	theme	sulfate	52:58	arg1	adsorbents					102:111	reusable and efficient adsorbents	79:111	reusable and efficient adsorbents of cationic dye methylene blue	79:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	0	42	theme	sulfate	52:58	arg1	beads					70:74	sulfate composite beads	52:74	sulfate composite beads	52:74	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	6	43	theme	pseudo	967:972	arg1	models					1022:1027	befitting models	1012:1027	befitting models	1012:1027	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	43	theme	pseudo	967:972	arg1	second-order					974:985	pseudo second-order	967:985	pseudo second-order	967:985	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	43	theme	pseudo	967:972	arg1	isotherm					1000:1007	Langmuir isotherm	991:1007	Langmuir isotherm	991:1007	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	10	44	from	adsorbents	1559:1568	arg1	treatment					1584:1592	wastewater treatment	1573:1592	wastewater treatment	1573:1592	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	7	45	theme	Langmuir	1042:1049	arg1	adsorption					1063:1072	The maximum Langmuir equilibrium adsorption	1030:1072	The maximum Langmuir equilibrium adsorption capacity (qm)	1030:1086	The maximum Langmuir equilibrium adsorption capacity (qm) was found to increase from 82 mg g-1 for C/D0 to 526 mg g-1 for C/D1.
30926505	4	46	theme	dye	659:661	arg1	methylene					663:671	cationic dye methylene blue	650:676	cationic dye methylene blue (MB)	650:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	46	theme	dye	659:661	arg1	MB					679:680	MB	679:680	MB	679:680	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	5	47	theme	DS	894:895	arg1	content					897:903	DS content	894:903	DS content	894:903	The results revealed that adsorption performances were remarkably improved by increasing DS content into beads.
30926505	6	48	theme	Langmuir	991:998	arg1	models					1022:1027	befitting models	1012:1027	befitting models	1012:1027	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	48	theme	Langmuir	991:998	arg1	second-order					974:985	pseudo second-order	967:985	pseudo second-order	967:985	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	48	theme	Langmuir	991:998	arg1	isotherm					1000:1007	Langmuir isotherm	991:1007	Langmuir isotherm	991:1007	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	7	49	theme	equilibrium	1051:1061	arg1	adsorption					1063:1072	The maximum Langmuir equilibrium adsorption	1030:1072	The maximum Langmuir equilibrium adsorption capacity (qm)	1030:1086	The maximum Langmuir equilibrium adsorption capacity (qm) was found to increase from 82 mg g-1 for C/D0 to 526 mg g-1 for C/D1.
30926505	1	50	theme	sodium	338:343	arg1	SDS					364:366	SDS	364:366	SDS	364:366	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	50	theme	sodium	338:343	arg1	sulfate					355:361	sodium n-dodecyl sulfate	338:361	sodium n-dodecyl sulfate (SDS)	338:367	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	6	51	theme	isotherm	930:937	arg1	studies					950:956	isotherm adsorption studies	930:956	isotherm adsorption studies	930:956	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	10	52	theme	recovery	1457:1464	arg1	view					1428:1431	view	1428:1431	view of easy preparation and recovery, effectiveness adsorption and good regeneration	1428:1512	In view of easy preparation and recovery, effectiveness adsorption and good regeneration, the composites could be applied as low-cost adsorbents in wastewater treatment.
30926505	4	53	theme	influencing	712:722	arg1	factors					724:730	influencing factors	712:730	influencing factors	712:730	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	1	54	theme	n-dodecyl	345:353	arg1	SDS					364:366	SDS	364:366	SDS	364:366	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	54	theme	n-dodecyl	345:353	arg1	sulfate					355:361	sodium n-dodecyl sulfate	338:361	sodium n-dodecyl sulfate (SDS)	338:367	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	7	55	theme	adsorption	1063:1072	arg1	qm					1084:1085	qm	1084:1085	qm	1084:1085	The maximum Langmuir equilibrium adsorption capacity (qm) was found to increase from 82 mg g-1 for C/D0 to 526 mg g-1 for C/D1.
30926505	7	55	theme	adsorption	1063:1072	arg1	capacity					1074:1081	The maximum Langmuir equilibrium adsorption capacity	1030:1081	The maximum Langmuir equilibrium adsorption capacity (qm)	1030:1086	The maximum Langmuir equilibrium adsorption capacity (qm) was found to increase from 82 mg g-1 for C/D0 to 526 mg g-1 for C/D1.
30926505	7	56	theme	maximum	1034:1040	arg1	adsorption					1063:1072	The maximum Langmuir equilibrium adsorption	1030:1072	The maximum Langmuir equilibrium adsorption capacity (qm)	1030:1086	The maximum Langmuir equilibrium adsorption capacity (qm) was found to increase from 82 mg g-1 for C/D0 to 526 mg g-1 for C/D1.
30926505	1	57	theme	Eco-friendly	145:156	arg1	beads					177:181	Eco-friendly hydrogel composite beads	145:181	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network	145:281	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	6	58	theme	befitting	1012:1020	arg1	models					1022:1027	befitting models	1012:1027	befitting models	1012:1027	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	58	theme	befitting	1012:1020	arg1	second-order					974:985	pseudo second-order	967:985	pseudo second-order	967:985	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	6	58	theme	befitting	1012:1020	arg1	isotherm					1000:1007	Langmuir isotherm	991:1007	Langmuir isotherm	991:1007	Kinetics and isotherm adsorption studies revealed pseudo second-order and Langmuir isotherm as befitting models.
30926505	1	59	theme	hydrogel	158:165	arg1	beads					177:181	Eco-friendly hydrogel composite beads	145:181	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network	145:281	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	60	theme	composite	167:175	arg1	beads					177:181	Eco-friendly hydrogel composite beads	145:181	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network	145:281	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	4	61	theme	blue	673:676	arg1	methylene					663:671	cationic dye methylene blue	650:676	cationic dye methylene blue (MB)	650:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	61	theme	blue	673:676	arg1	MB					679:680	MB	679:680	MB	679:680	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	4	62	theme	composites	635:644	arg1	adsorption					621:630	adsorption	621:630	adsorption of composites for cationic dye methylene blue (MB)	621:681	The effect of DS on adsorption of composites for cationic dye methylene blue (MB) was investigated by changing influencing factors: pH, adsorbent dosage, time contact, dye concentration, and temperature.
30926505	0	63	theme	reusable	79:86	arg1	adsorbents					102:111	reusable and efficient adsorbents	79:111	reusable and efficient adsorbents of cationic dye methylene blue	79:142	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	0	63	theme	reusable	79:86	arg1	beads					70:74	sulfate composite beads	52:74	sulfate composite beads	52:74	Eco-friendly porous carboxymethyl cellulose/dextran sulfate composite beads as reusable and efficient adsorbents of cationic dye methylene blue.
30926505	1	64	theme	sulfate	355:361	arg1	presence					326:333	presence	326:333	presence of sodium n-dodecyl sulfate (SDS)	326:367	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	2	65	theme	TGA	465:467	arg1	analyses					469:476	TGA analyses	465:476	TGA analyses	465:476	The milligels composites C/Dx were characterized by FTIR, SEM/EDX and TGA analyses.
30926505	1	66	theme	embedded	259:266	arg1	CMC					229:231	CMC	229:231	CMC	229:231	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30926505	1	66	theme	embedded	259:266	arg1	cellulose					218:226	crosslinked-carboxymethyl cellulose	192:226	crosslinked-carboxymethyl cellulose (CMC)	192:232	Eco-friendly hydrogel composite beads based on crosslinked-carboxymethyl cellulose (CMC) and dextran sulfate (DS) embedded within network were prepared using ionotropic gelation in presence of sodium n-dodecyl sulfate (SDS) as pore-forming template.
30269844	4	0	theme	RAM	966:968	arg1	extracts					948:955	polysaccharide extracts	933:955	polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt)	933:1022	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	3	1	theme	IEC-6	538:542	arg1	cells					544:548	IEC-6 cells	538:548	IEC-6 cells	538:548	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	8	2	theme	accelerated	1591:1601	arg1	migration					1603:1611	accelerated migration	1591:1611	accelerated migration of IEC-6 cells	1591:1626	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	12	3	theme	intestinal	2314:2323	arg1	cells					2336:2340	intestinal epithelial cells	2314:2340	intestinal epithelial cells	2314:2340	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	4	4	theme	RA	869:870	arg1	4-Aminopyridine					801:815	4-Aminopyridine	801:815	4-Aminopyridine (40 μmol·L-1, 4-AP)	801:835	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	μmol·L-1					733:740	5 μmol·L-1	731:740	5 μmol·L-1	731:740	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	DFMO					905:908	DFMO	905:908	DFMO	905:908	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	mmol·L-1					784:791	2.5 mmol·L-1	780:791	2.5 mmol·L-1	780:791	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	SPD					915:917	SPD	915:917	SPD	915:917	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	spermidine					719:728	spermidine	719:728	spermidine (5 μmol·L-1, SPD)	719:746	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	μmol·L-1					821:828	40 μmol·L-1	818:828	40 μmol·L-1	818:828	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	extracts					857:864	the polysaccharide extracts	838:864	the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1)	838:902	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	alpha-difluoromethylornithine					749:777	alpha-difluoromethylornithine	749:777	alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO)	749:798	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	4	theme	RA	869:870	arg1	DFMO					923:926	DFMO	923:926	DFMO	923:926	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	10	5	theme	P	1915:1915	arg1	<					1917:1917	P < 0.05	1915:1922	P < 0.05	1915:1922	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	8	6	theme	polyamine	1549:1557	arg1	content					1559:1565	cellular polyamine content	1540:1565	cellular polyamine content	1540:1565	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	9	7	theme	control	1828:1834	arg1	level					1836:1840	control level	1828:1840	control level	1828:1840	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	3	8	theme	single-edged	570:581	arg1	blade					589:593	a single-edged razor blade	568:593	a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates	568:658	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	11	9	theme	Molar	2042:2046	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	4	10	theme	[Ca2+	1013:1017	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	8	11	theme	cells	1622:1626	arg1	cyt					1583:1585	elevated [Ca2+]cyt	1568:1585	elevated [Ca2+]cyt	1568:1585	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	8	11	theme	cells	1622:1626	arg1	content					1559:1565	cellular polyamine content	1540:1565	cellular polyamine content	1540:1565	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	8	11	theme	cells	1622:1626	arg1	migration					1603:1611	accelerated migration	1591:1611	accelerated migration of IEC-6 cells	1591:1626	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	1	12	used	used	303:306	arg2	Astragali					206:214	Astragali	206:214	Astragali	206:214	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	1	12	used	used	303:306	arg2	membranaceus					186:197	Astragalus membranaceus	175:197	Astragalus membranaceus (Radix Astragali, RA)	175:219	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	1	12	used	used	303:306	arg2	macrocephala					238:249	macrocephala	238:249	macrocephala	238:249	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	3	13	with	model	527:531	arg1	cells					544:548	IEC-6 cells	538:548	IEC-6 cells	538:548	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	2	14	from	effects	384:390	arg1	herbs					435:439	these two herbs	425:439	these two herbs	425:439	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	2	14	from	effects	384:390	arg1	migration					455:463	IEC-6 cell migration	444:463	IEC-6 cell migration	444:463	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	4	15	theme	cytosolic	992:1000	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	0	16	theme	polyamine-mediated	144:161	arg1	channel					166:172	the polyamine-mediated K+ channel	140:172	the polyamine-mediated K+ channel	140:172	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	4	17	dep	μmol·L-1	821:828	arg1	4-AP					831:834	4-AP	831:834	4-AP	831:834	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	12	18	theme	polysaccharide	2266:2279	arg1	extracts					2281:2288	RAM polysaccharide extracts	2262:2288	RAM polysaccharide extracts	2262:2288	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	4	19	theme	Ca2+	1007:1010	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	3	20	theme	layers	622:627	arg1	diameter					605:612	the diameter	601:612	the diameter of cell layers	601:627	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	4	21	theme	RAM	875:877	arg1	4-Aminopyridine					801:815	4-Aminopyridine	801:815	4-Aminopyridine (40 μmol·L-1, 4-AP)	801:835	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	μmol·L-1					733:740	5 μmol·L-1	731:740	5 μmol·L-1	731:740	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	DFMO					905:908	DFMO	905:908	DFMO	905:908	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	mmol·L-1					784:791	2.5 mmol·L-1	780:791	2.5 mmol·L-1	780:791	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	SPD					915:917	SPD	915:917	SPD	915:917	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	spermidine					719:728	spermidine	719:728	spermidine (5 μmol·L-1, SPD)	719:746	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	μmol·L-1					821:828	40 μmol·L-1	818:828	40 μmol·L-1	818:828	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	extracts					857:864	the polysaccharide extracts	838:864	the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1)	838:902	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	alpha-difluoromethylornithine					749:777	alpha-difluoromethylornithine	749:777	alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO)	749:798	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	21	theme	RAM	875:877	arg1	DFMO					923:926	DFMO	923:926	DFMO	923:926	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	22	theme	confocal	1049:1056	arg1	microscopy					1058:1067	laser confocal microscopy	1043:1067	laser confocal microscopy	1043:1067	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	8	23	theme	<	1659:1659	arg1	P					1657:1657	P < 0.01	1657:1664	P < 0.01	1657:1664	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	8	23	theme	<	1659:1659	arg1	controls					1647:1654	the controls	1643:1654	the controls (P < 0.01)	1643:1665	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	12	24	theme	injury	2430:2435	arg1	healing					2437:2443	intestinal injury healing	2419:2443	intestinal injury healing	2419:2443	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	3	25	from	blade	589:593	arg1	plates					653:658	six-well polystyrene plates	632:658	six-well polystyrene plates	632:658	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	11	26	theme	D-ribose	2058:2065	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	12	27	theme	RAM	2262:2264	arg1	extracts					2281:2288	RAM polysaccharide extracts	2262:2288	RAM polysaccharide extracts	2262:2288	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	11	28	from	2.2	2213:2215	arg1	RAM					2220:2222	RAM	2220:2222	RAM	2220:2222	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	6	29	theme	migration	1268:1276	arg1	assay					1278:1282	A cell migration assay	1261:1282	A cell migration assay	1261:1282	A cell migration assay was carried out using Image-Pro Plus software.
30269844	8	30	with	treatment	1464:1472	arg1	extracts					1494:1501	polysaccharide extracts	1479:1501	polysaccharide extracts	1479:1501	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	9	31	dep	restored	1795:1802	arg1	<					1845:1845	P < 0.01	1843:1850	P < 0.01	1843:1850	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	4	32	dep	mmol·L-1	784:791	arg1	DFMO					794:797	DFMO	794:797	DFMO	794:797	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	5	33	theme	mRNA	1133:1136	arg1	expression					1138:1147	Kv1.1 mRNA expression	1127:1147	Kv1.1 mRNA expression	1127:1147	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	10	34	theme	protein	1879:1885	arg1	expressions					1887:1897	protein expressions	1879:1897	protein expressions	1879:1897	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	0	35	theme	epithelial	100:109	arg1	migration					116:124	intestinal epithelial cell migration	89:124	intestinal epithelial cell migration	89:124	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	11	36	theme	L-rhamnose	2081:2090	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	11	37	theme	D-arabinose	2068:2078	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	4	38	theme	cellular	1074:1081	arg1	content					1093:1099	cellular polyamine content	1074:1099	cellular polyamine content	1074:1099	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	2	39	theme	extracts	411:418	arg1	effects					384:390	the effects	380:390	the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration	380:463	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	0	40	theme	Polysaccharide	0:13	arg1	extracts					15:22	Polysaccharide extracts	0:22	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala	0:79	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	4	41	dep	μmol·L-1	733:740	arg1	SPD					743:745	SPD	743:745	SPD	743:745	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	12	42	theme	cells	2336:2340	arg1	migration					2301:2309	migration	2301:2309	migration of intestinal epithelial cells	2301:2340	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	2	43	theme	IEC-6	444:448	arg1	migration					455:463	IEC-6 cell migration	444:463	IEC-6 cell migration	444:463	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	12	44	theme	signaling	2382:2390	arg1	pathway					2392:2398	a polyamine-Kv1.1 channel activated signaling pathway	2346:2398	a polyamine-Kv1.1 channel activated signaling pathway	2346:2398	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	7	45	theme	extracts	1430:1437	arg1	composition					1395:1405	the monosaccharide composition	1376:1405	the monosaccharide composition of both polysaccharide extracts	1376:1437	In addition, GC-MS was introduced to analyze the monosaccharide composition of both polysaccharide extracts.
30269844	10	46	theme	extract	1946:1952	arg1	treatment					1954:1962	polysaccharide extract treatment	1931:1962	polysaccharide extract treatment	1931:1962	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	5	47	theme	protein	1195:1201	arg1	expressions					1203:1213	RhoA protein expressions	1190:1213	RhoA protein expressions	1190:1213	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	0	48	theme	membranaceus	38:49	arg1	extracts					15:22	Polysaccharide extracts	0:22	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala	0:79	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	2	49	theme	underlying	492:501	arg1	mechanisms					503:512	the potential underlying mechanisms	478:512	the potential underlying mechanisms	478:512	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	4	50	theme	polysaccharide	933:946	arg1	extracts					948:955	polysaccharide extracts	933:955	polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt)	933:1022	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	1	51	dep	Astragali	206:214	arg1	RA					217:218	RA	217:218	RA	217:218	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	3	52	theme	migration	517:525	arg1	model					527:531	A migration model	515:531	A migration model with IEC-6 cells	515:548	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	0	53	theme	Atractylodes	55:66	arg1	macrocephala					68:79	Atractylodes macrocephala	55:79	Atractylodes macrocephala	55:79	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	9	54	theme	DFMO	1736:1739	arg1	effects					1725:1731	the inhibitory effects	1710:1731	the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt	1710:1783	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	8	55	theme	polysaccharide	1479:1492	arg1	extracts					1494:1501	polysaccharide extracts	1479:1501	polysaccharide extracts	1479:1501	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	1	56	dep	macrocephala	238:249	arg1	Macrocephalae					273:285	Macrocephalae	273:285	Macrocephalae	273:285	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	1	56	dep	macrocephala	238:249	arg1	RAM					288:290	RAM	288:290	RAM	288:290	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	2	57	from	herbs	435:439	arg1	effects					384:390	the effects	380:390	the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration	380:463	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	2	57	from	herbs	435:439	arg1	extracts					411:418	polysaccharides extracts	395:418	polysaccharides extracts from these two herbs	395:439	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	9	58	theme	cellular	1744:1751	arg1	content					1763:1769	cellular polyamine content	1744:1769	cellular polyamine content	1744:1769	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	11	59	theme	D-mannose	2093:2101	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	5	60	theme	blotting	1242:1249	arg1	analysis					1251:1258	Western blotting analysis	1234:1258	Western blotting analysis	1234:1258	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	8	61	theme	elevated	1568:1575	arg1	cyt					1583:1585	elevated [Ca2+]cyt	1568:1585	elevated [Ca2+]cyt	1568:1585	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	2	62	theme	potential	482:490	arg1	mechanisms					503:512	the potential underlying mechanisms	478:512	the potential underlying mechanisms	478:512	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	9	63	from	effects	1725:1731	arg1	cyt					1781:1783	[Ca2+]cyt	1775:1783	[Ca2+]cyt	1775:1783	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	9	63	from	effects	1725:1731	arg1	content					1763:1769	cellular polyamine content	1744:1769	cellular polyamine content	1744:1769	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	6	64	theme	cell	1263:1266	arg1	assay					1278:1282	A cell migration assay	1261:1282	A cell migration assay	1261:1282	A cell migration assay was carried out using Image-Pro Plus software.
30269844	4	65	theme	polysaccharide	842:855	arg1	extracts					857:864	the polysaccharide extracts	838:864	the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1)	838:902	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	9	66	theme	[Ca2+	1775:1779	arg1	cyt					1781:1783	[Ca2+]cyt	1775:1783	[Ca2+]cyt	1775:1783	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	10	67	theme	protein	2008:2014	arg1	expression					2016:2025	RhoA protein expression	2003:2025	RhoA protein expression	2003:2025	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	11	68	theme	D-glucose	2104:2112	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	8	69	theme	cellular	1540:1547	arg1	content					1559:1565	cellular polyamine content	1540:1565	cellular polyamine content	1540:1565	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	12	70	theme	epithelial	2325:2334	arg1	cells					2336:2340	intestinal epithelial cells	2314:2340	intestinal epithelial cells	2314:2340	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	10	71	theme	polysaccharide	1931:1944	arg1	treatment					1954:1962	polysaccharide extract treatment	1931:1962	polysaccharide extract treatment	1931:1962	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	8	72	theme	IEC-6	1616:1620	arg1	cells					1622:1626	IEC-6 cells	1616:1626	IEC-6 cells	1616:1626	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	4	73	theme	free	1002:1005	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	74	theme	control	685:691	arg1	media					693:697	control media	685:697	control media	685:697	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	0	75	theme	K+	163:164	arg1	channel					166:172	the polyamine-mediated K+ channel	140:172	the polyamine-mediated K+ channel	140:172	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	3	76	theme	razor	583:587	arg1	blade					589:593	a single-edged razor blade	568:593	a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates	568:658	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	9	77	theme	cell	1810:1813	arg1	migration					1815:1823	IEC-6 cell migration	1804:1823	IEC-6 cell migration	1804:1823	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	3	78	theme	cell	617:620	arg1	layers					622:627	cell layers	617:627	cell layers	617:627	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	9	79	dep	<	1845:1845	arg1	<					1855:1855	< 0.05	1855:1860	< 0.05	1855:1860	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	12	80	with	treatment	2240:2248	arg1	RA					2255:2256	RA	2255:2256	RA	2255:2256	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	12	80	with	treatment	2240:2248	arg1	extracts					2281:2288	RAM polysaccharide extracts	2262:2288	RAM polysaccharide extracts	2262:2288	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	12	81	theme	intestinal	2419:2428	arg1	healing					2437:2443	intestinal injury healing	2419:2443	intestinal injury healing	2419:2443	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	10	82	theme	RhoA	2003:2006	arg1	expression					2016:2025	RhoA protein expression	2003:2025	RhoA protein expression	2003:2025	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	10	83	dep	increased	1904:1912	arg1	<					1917:1917	P < 0.05	1915:1922	P < 0.05	1915:1922	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	3	84	theme	polystyrene	641:651	arg1	plates					653:658	six-well polystyrene plates	632:658	six-well polystyrene plates	632:658	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	9	85	theme	P	1843:1843	arg1	<					1845:1845	P < 0.01	1843:1850	P < 0.01	1843:1850	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	9	86	theme	IEC-6	1804:1808	arg1	migration					1815:1823	IEC-6 cell migration	1804:1823	IEC-6 cell migration	1804:1823	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	0	87	theme	intestinal	89:98	arg1	migration					116:124	intestinal epithelial cell migration	89:124	intestinal epithelial cell migration	89:124	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	1	88	theme	Astragalus	175:184	arg1	membranaceus					186:197	Astragalus membranaceus	175:197	Astragalus membranaceus (Radix Astragali, RA)	175:219	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	1	88	theme	Astragalus	175:184	arg1	Astragali					206:214	Astragali	206:214	Astragali	206:214	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	4	89	theme	laser	1043:1047	arg1	microscopy					1058:1067	laser confocal microscopy	1043:1067	laser confocal microscopy	1043:1067	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	5	90	theme	Kv1.1	1127:1131	arg1	expression					1138:1147	Kv1.1 mRNA expression	1127:1147	Kv1.1 mRNA expression	1127:1147	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	0	91	theme	cell	111:114	arg1	migration					116:124	intestinal epithelial cell migration	89:124	intestinal epithelial cell migration	89:124	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	3	92	theme	six-well	632:639	arg1	plates					653:658	six-well polystyrene plates	632:658	six-well polystyrene plates	632:658	A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates.
30269844	12	93	theme	activated	2372:2380	arg1	pathway					2392:2398	a polyamine-Kv1.1 channel activated signaling pathway	2346:2398	a polyamine-Kv1.1 channel activated signaling pathway	2346:2398	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	10	94	theme	polyamine-deficient	1967:1985	arg1	cells					1993:1997	polyamine-deficient IEC-6 cells	1967:1997	polyamine-deficient IEC-6 cells	1967:1997	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	10	95	theme	Kv1.1	1864:1868	arg1	mRNA					1870:1873	Kv1.1 mRNA	1864:1873	Kv1.1 mRNA	1864:1873	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30269844	4	96	dep	RAM	966:968	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	9	97	theme	Polysaccharide	1668:1681	arg1	extracts					1683:1690	Polysaccharide extracts	1668:1690	Polysaccharide extracts	1668:1690	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	4	98	theme	polyamine	1083:1091	arg1	content					1093:1099	cellular polyamine content	1074:1099	cellular polyamine content	1074:1099	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	2	99	theme	polysaccharides	395:409	arg1	extracts					411:418	polysaccharides extracts	395:418	polysaccharides extracts from these two herbs	395:439	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	0	100	theme	Astragalus	27:36	arg1	membranaceus					38:49	Astragalus membranaceus	27:49	Astragalus membranaceus	27:49	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	2	101	theme	cell	450:453	arg1	migration					455:463	IEC-6 cell migration	444:463	IEC-6 cell migration	444:463	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	11	102	theme	D-galactose	2119:2129	arg1	ratios					2048:2053	Molar ratios	2042:2053	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose	2042:2129	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	5	103	theme	RhoA	1190:1193	arg1	expressions					1203:1213	RhoA protein expressions	1190:1213	RhoA protein expressions	1190:1213	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	1	104	theme	gastrointestinal	317:332	arg1	diseases					334:341	gastrointestinal diseases	317:341	gastrointestinal diseases	317:341	Astragalus membranaceus (Radix Astragali, RA) and Atractylodes macrocephala (Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases.
30269844	4	105	theme	Next	986:989	arg1	cyt					1019:1021	Next, cytosolic free Ca2+ ([Ca2+]cyt	986:1021	cyt	1019:1021	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	9	106	theme	inhibitory	1714:1723	arg1	effects					1725:1731	the inhibitory effects	1710:1731	the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt	1710:1783	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	0	107	theme	macrocephala	68:79	arg1	extracts					15:22	Polysaccharide extracts	0:22	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala	0:79	Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K+ channel.
30269844	7	108	theme	polysaccharide	1415:1428	arg1	extracts					1430:1437	both polysaccharide extracts	1410:1437	both polysaccharide extracts	1410:1437	In addition, GC-MS was introduced to analyze the monosaccharide composition of both polysaccharide extracts.
30269844	4	109	theme	RA	960:961	arg1	extracts					948:955	polysaccharide extracts	933:955	polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt)	933:1022	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	8	110	theme	RA	1506:1507	arg1	treatment					1464:1472	treatment	1464:1472	treatment with polysaccharide extracts of RA or RAM	1464:1514	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	5	111	theme	Western	1234:1240	arg1	analysis					1251:1258	Western blotting analysis	1234:1258	Western blotting analysis	1234:1258	Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis.
30269844	2	112	theme	present	351:357	arg1	study					359:363	the present study	347:363	the present study	347:363	In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms.
30269844	8	113	theme	[Ca2+	1577:1581	arg1	cyt					1583:1585	elevated [Ca2+]cyt	1568:1585	elevated [Ca2+]cyt	1568:1585	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	7	114	theme	monosaccharide	1380:1393	arg1	composition					1395:1405	the monosaccharide composition	1376:1405	the monosaccharide composition of both polysaccharide extracts	1376:1437	In addition, GC-MS was introduced to analyze the monosaccharide composition of both polysaccharide extracts.
30269844	9	115	theme	polyamine	1753:1761	arg1	content					1763:1769	cellular polyamine content	1744:1769	cellular polyamine content	1744:1769	Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca2+]cyt, but also restored IEC-6 cell migration to control level (P < 0.01 or < 0.05).
30269844	8	116	theme	RAM	1512:1514	arg1	treatment					1464:1472	treatment	1464:1472	treatment with polysaccharide extracts of RA or RAM	1464:1514	The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca2+]cyt and accelerated migration of IEC-6 cells, compared with the controls (P < 0.01).
30269844	4	117	contain	containing	708:717	arg2	extracts					857:864	the polysaccharide extracts	838:864	the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1)	838:902	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	μmol·L-1					821:828	40 μmol·L-1	818:828	40 μmol·L-1	818:828	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	spermidine					719:728	spermidine	719:728	spermidine (5 μmol·L-1, SPD)	719:746	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	alpha-difluoromethylornithine					749:777	alpha-difluoromethylornithine	749:777	alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO)	749:798	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	DFMO					923:926	DFMO	923:926	DFMO	923:926	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	μmol·L-1					733:740	5 μmol·L-1	731:740	5 μmol·L-1	731:740	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	4-Aminopyridine					801:815	4-Aminopyridine	801:815	4-Aminopyridine (40 μmol·L-1, 4-AP)	801:835	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	DFMO					905:908	DFMO	905:908	DFMO	905:908	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg2	mmol·L-1					784:791	2.5 mmol·L-1	780:791	2.5 mmol·L-1	780:791	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	4	117	contain	containing	708:717	arg1	media					693:697	control media	685:697	control media	685:697	The cells were grown in control media or media containing spermidine (5 μmol·L-1, SPD), alpha-difluoromethylornithine (2.5 mmol·L-1, DFMO), 4-Aminopyridine (40 μmol·L-1, 4-AP), the polysaccharide extracts of RA or RAM (50, 100, or 200 mg·L-1), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca2+ ([Ca2+]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC.
30269844	11	118	from	6.3	2169:2171	arg1	RA					2176:2177	RA	2176:2177	RA	2176:2177	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	11	118	from	6.3	2169:2171	arg1	2.2					2213:2215	2.2	2213:2215	2.2	2213:2215	Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0 : 14.1 : 0.3 : 19.9 : 181.3 : 6.3 in RA and 1.0 : 4.3 : 0.1 : 5.7 : 2.8 : 2.2 in RAM.
30269844	12	119	dep	activated	2372:2380	arg1	channel					2364:2370	channel	2364:2370	channel	2364:2370	In conclusion, treatment with RA and RAM polysaccharide extracts stimulated migration of intestinal epithelial cells via a polyamine-Kv1.1 channel activated signaling pathway, which facilitated intestinal injury healing.
30269844	10	120	theme	IEC-6	1987:1991	arg1	cells					1993:1997	polyamine-deficient IEC-6 cells	1967:1997	polyamine-deficient IEC-6 cells	1967:1997	Kv1.1 mRNA and protein expressions were increased (P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased.
30531893	8	0	theme	microbial	1542:1550	arg1	community					1552:1560	a pelagic microbial community	1532:1560	a pelagic microbial community	1532:1560	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	1	1	from	cycling	306:312	arg1	ocean					321:325	the ocean	317:325	the ocean	317:325	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	7	2	theme	bacteria	1434:1441	arg1	rate					1417:1420	the growth rate	1406:1420	the growth rate of specific bacteria	1406:1441	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	6	3	theme	Sharing	1072:1078	arg1	producing					1103:1111	extracellular enzyme producing	1082:1111	extracellular enzyme producing	1082:1111	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	3	4	theme	processing	681:690	arg1	mode					663:666	a newly-identified 'selfish' mode	634:666	a newly-identified 'selfish' mode of substrate processing	634:690	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	3	5	theme	newly-identified	636:651	arg1	mode					663:666	a newly-identified 'selfish' mode	634:666	a newly-identified 'selfish' mode of substrate processing	634:690	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	1	6	theme	substrates	240:249	arg1	degradation					197:207	the degradation	193:207	the degradation of high molecular weight (HMW) substrates	193:249	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	7	7	theme	individual	1263:1272	arg1	mechanisms					1274:1283	individual mechanisms	1263:1283	individual mechanisms	1263:1283	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	0	8	from	Selfish	0:6	arg1	Ocean					57:61	the Atlantic Ocean	44:61	the Atlantic Ocean	44:61	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	7	9	from	structure	1328:1336	arg1	location					1376:1383	a given location	1368:1383	a given location	1368:1383	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	7	9	from	structure	1328:1336	arg1	time					1389:1392	time	1389:1392	time	1389:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	0	10	theme	biogeographical	66:80	arg1	study					82:86	a biogeographical study	64:86	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.	0:122	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	8	11	theme	significant	1575:1585	arg1	effects					1597:1603	significant follow-on effects	1575:1603	significant follow-on effects for carbon cycling	1575:1622	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	7	12	theme	population	1317:1326	arg1	structure					1328:1336	the initial population structure	1305:1336	the initial population structure of the bacterial community at a given location and time	1305:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	4	13	theme	mechanisms	776:785	arg1	balance					732:738	the balance	728:738	the balance of individual substrate utilisation mechanisms	728:785	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	0	14	theme	bacterial	91:99	arg1	utilisation					111:121	bacterial substrate utilisation	91:121	bacterial substrate utilisation	91:121	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	2	15	from	changes	426:432	arg1	composition					457:467	microbial community composition	437:467	microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time	437:530	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	6	16	theme	non-enzyme	1132:1141	arg1	producing					1143:1151	non-enzyme producing	1132:1151	non-enzyme producing	1132:1151	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	6	16	theme	non-enzyme	1132:1141	arg1	scavenging					1119:1128	'scavenging'	1118:1129	'scavenging' (non-enzyme producing)	1118:1152	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	1	17	theme	individual	156:165	arg1	bacteria					181:188	individual heterotrophic bacteria	156:188	individual heterotrophic bacteria	156:188	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	0	18	from	bacteria	32:39	arg1	Ocean					57:61	the Atlantic Ocean	44:61	the Atlantic Ocean	44:61	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	1	19	from	constraints	284:294	arg1	cycling					306:312	carbon cycling	299:312	carbon cycling in the ocean	299:325	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	2	20	from	sites	336:340	arg1	Ocean					358:362	the Atlantic Ocean	345:362	the Atlantic Ocean	345:362	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	4	21	theme	different	790:798	arg1	locations					800:808	different locations	790:808	different locations	790:808	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	1	22	theme	heterotrophic	167:179	arg1	bacteria					181:188	individual heterotrophic bacteria	156:188	individual heterotrophic bacteria	156:188	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	2	23	theme	tracking	417:424	arg1	changes					426:432	tracking changes	417:432	tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time	417:530	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	8	24	theme	follow-on	1587:1595	arg1	effects					1597:1603	significant follow-on effects	1575:1603	significant follow-on effects for carbon cycling	1575:1622	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	4	25	theme	individual	743:752	arg1	mechanisms					776:785	individual substrate utilisation mechanisms	743:785	individual substrate utilisation mechanisms	743:785	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	8	26	theme	carbon	1609:1614	arg1	cycling					1616:1622	carbon cycling	1609:1622	carbon cycling	1609:1622	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	7	27	theme	growth	1410:1415	arg1	rate					1417:1420	the growth rate	1406:1420	the growth rate of specific bacteria	1406:1441	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	2	28	theme	Atlantic	349:356	arg1	Ocean					358:362	the Atlantic Ocean	345:362	the Atlantic Ocean	345:362	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	2	29	theme	matter	407:412	arg1	processing					385:394	the processing	381:394	the processing of organic matter	381:412	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	0	30	theme	utilisation	111:121	arg1	study					82:86	a biogeographical study	64:86	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.	0:122	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	7	31	theme	specific	1425:1432	arg1	bacteria					1434:1441	specific bacteria	1425:1441	specific bacteria	1425:1441	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	4	32	theme	utilisation	764:774	arg1	mechanisms					776:785	individual substrate utilisation mechanisms	743:785	individual substrate utilisation mechanisms	743:785	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	4	33	theme	distinct	850:857	arg1	mechanisms					881:890	distinct substrate utilisation mechanisms	850:890	distinct substrate utilisation mechanisms	850:890	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	2	34	theme	organic	399:405	arg1	matter					407:412	organic matter	399:412	organic matter	399:412	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	0	35	theme	substrate	101:109	arg1	utilisation					111:121	bacterial substrate utilisation	91:121	bacterial substrate utilisation	91:121	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	4	36	theme	substrate	754:762	arg1	mechanisms					776:785	individual substrate utilisation mechanisms	743:785	individual substrate utilisation mechanisms	743:785	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	2	37	theme	community	447:455	arg1	composition					457:467	microbial community composition	437:467	microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time	437:530	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	7	38	theme	given	1370:1374	arg1	location					1376:1383	a given location	1368:1383	a given location	1368:1383	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	3	39	theme	selfish	654:660	arg1	mode					663:666	a newly-identified 'selfish' mode	634:666	a newly-identified 'selfish' mode of substrate processing	634:690	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	3	40	theme	considerable	581:592	arg1	fraction					594:601	a considerable fraction	579:601	a considerable fraction of heterotrophic bacteria	579:627	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	5	41	theme	fluorescently-labelled	920:941	arg1	polysaccharides					943:957	fluorescently-labelled polysaccharides	920:957	fluorescently-labelled polysaccharides	920:957	Through FISH and uptake of fluorescently-labelled polysaccharides, 'selfish' organisms were identified as belonging to the Bacteroidetes, Planctomycetes and Gammaproteobacteria.
30531893	0	42	theme	scavenging	21:30	arg1	bacteria					32:39	scavenging bacteria	21:39	scavenging bacteria	21:39	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	2	43	theme	microbial	437:445	arg1	composition					457:467	microbial community composition	437:467	microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time	437:530	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	8	44	theme	different	1511:1519	arg1	members					1521:1527	different members	1511:1527	different members of a pelagic microbial community	1511:1560	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	1	45	theme	high	212:215	arg1	HMW					235:237	HMW	235:237	HMW	235:237	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	1	45	theme	high	212:215	arg1	weight					227:232	high molecular weight	212:232	high molecular weight (HMW) substrates	212:249	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	1	46	theme	carbon	299:304	arg1	cycling					306:312	carbon cycling	299:312	carbon cycling in the ocean	299:325	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	0	47	theme	Atlantic	48:55	arg1	Ocean					57:61	the Atlantic Ocean	44:61	the Atlantic Ocean	44:61	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	4	48	theme	utilisation	869:879	arg1	mechanisms					881:890	distinct substrate utilisation mechanisms	850:890	distinct substrate utilisation mechanisms	850:890	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	6	49	theme	Alteromonadaceae	1194:1209	arg1	clades					1221:1226	the Alteromonadaceae and SAR11 clades	1190:1226	clades	1221:1226	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	8	50	theme	same	1461:1464	arg1	substrate					1466:1474	the same substrate	1457:1474	the same substrate	1457:1474	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	2	51	dep	composition	457:467	arg1	hydrolysed					511:520	hydrolysed	511:520	were enzymatically hydrolysed over time	492:530	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	4	52	theme	substrate	859:867	arg1	mechanisms					881:890	distinct substrate utilisation mechanisms	850:890	distinct substrate utilisation mechanisms	850:890	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	7	53	theme	community	1355:1363	arg1	structure					1328:1336	the initial population structure	1305:1336	the initial population structure of the bacterial community at a given location and time	1305:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	8	54	theme	different	1493:1501	arg1	ways					1503:1506	different ways	1493:1506	different ways	1493:1506	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	3	55	theme	heterotrophic	606:618	arg1	bacteria					620:627	heterotrophic bacteria	606:627	heterotrophic bacteria	606:627	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	3	56	theme	substrate	671:679	arg1	processing					681:690	substrate processing	671:690	substrate processing	671:690	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	3	57	theme	bacteria	620:627	arg1	fraction					594:601	a considerable fraction	579:601	a considerable fraction of heterotrophic bacteria	579:627	During this investigation, we discovered that a considerable fraction of heterotrophic bacteria uses a newly-identified 'selfish' mode of substrate processing.
30531893	6	58	theme	extracellular	1082:1094	arg1	producing					1103:1111	extracellular enzyme producing	1082:1111	extracellular enzyme producing	1082:1111	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	5	59	theme	selfish	961:967	arg1	organisms					970:978	'selfish' organisms	960:978	'selfish' organisms	960:978	Through FISH and uptake of fluorescently-labelled polysaccharides, 'selfish' organisms were identified as belonging to the Bacteroidetes, Planctomycetes and Gammaproteobacteria.
30531893	6	60	theme	SAR11	1215:1219	arg1	clades					1221:1226	the Alteromonadaceae and SAR11 clades	1190:1226	clades	1221:1226	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	1	61	theme	molecular	217:225	arg1	HMW					235:237	HMW	235:237	HMW	235:237	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	1	61	theme	molecular	217:225	arg1	weight					227:232	high molecular weight	212:232	high molecular weight (HMW) substrates	212:249	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	8	62	theme	pelagic	1534:1540	arg1	community					1552:1560	a pelagic microbial community	1532:1560	a pelagic microbial community	1532:1560	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	6	63	theme	scavenging	1119:1128	arg1	organisms					1154:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms	1071:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms	1071:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	7	64	from	time	1389:1392	arg1	structure					1328:1336	the initial population structure	1305:1336	the initial population structure of the bacterial community at a given location and time	1305:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	7	64	from	time	1389:1392	arg1	community					1355:1363	the bacterial community	1341:1363	the bacterial community at a given location and time	1341:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	7	65	theme	bacterial	1345:1353	arg1	community					1355:1363	the bacterial community	1341:1363	the bacterial community at a given location and time	1341:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	1	66	theme	weight	227:232	arg1	substrates					240:249	high molecular weight (HMW) substrates	212:249	high molecular weight (HMW) substrates	212:249	Identifying the roles played by individual heterotrophic bacteria in the degradation of high molecular weight (HMW) substrates is critical to understanding the constraints on carbon cycling in the ocean.
30531893	7	67	from	location	1376:1383	arg1	structure					1328:1336	the initial population structure	1305:1336	the initial population structure of the bacterial community at a given location and time	1305:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	7	67	from	location	1376:1383	arg1	community					1355:1363	the bacterial community	1341:1363	the bacterial community at a given location and time	1341:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
30531893	6	68	theme	producing	1103:1111	arg1	organisms					1154:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms	1071:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms	1071:1162	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	4	69	theme	individual	821:830	arg1	microorganisms					832:845	individual microorganisms	821:845	individual microorganisms	821:845	We therefore additionally examined the balance of individual substrate utilisation mechanisms at different locations by linking individual microorganisms to distinct substrate utilisation mechanisms.
30531893	5	70	theme	polysaccharides	943:957	arg1	uptake					910:915	uptake	910:915	uptake of fluorescently-labelled polysaccharides	910:957	Through FISH and uptake of fluorescently-labelled polysaccharides, 'selfish' organisms were identified as belonging to the Bacteroidetes, Planctomycetes and Gammaproteobacteria.
30531893	5	70	theme	polysaccharides	943:957	arg1	FISH					901:904	FISH	901:904	FISH	901:904	Through FISH and uptake of fluorescently-labelled polysaccharides, 'selfish' organisms were identified as belonging to the Bacteroidetes, Planctomycetes and Gammaproteobacteria.
30531893	2	71	theme	HMW	472:474	arg1	polysaccharides					476:490	HMW polysaccharides	472:490	HMW polysaccharides	472:490	At five sites in the Atlantic Ocean, we investigated the processing of organic matter by tracking changes in microbial community composition as HMW polysaccharides were enzymatically hydrolysed over time.
30531893	8	72	theme	community	1552:1560	arg1	members					1521:1527	different members	1511:1527	different members of a pelagic microbial community	1511:1560	Furthermore, the same substrate was processed in different ways by different members of a pelagic microbial community, pointing to significant follow-on effects for carbon cycling.
30531893	0	73	dep	Selfish	0:6	arg1	study					82:86	a biogeographical study	64:86	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.	0:122	Selfish, sharing and scavenging bacteria in the Atlantic Ocean: a biogeographical study of bacterial substrate utilisation.
30531893	6	74	theme	enzyme	1096:1101	arg1	producing					1103:1111	extracellular enzyme producing	1082:1111	extracellular enzyme producing	1082:1111	'Sharing' (extracellular enzyme producing) and 'scavenging' (non-enzyme producing) organisms predominantly belonged to the Alteromonadaceae and SAR11 clades, respectively.
30531893	7	75	theme	initial	1309:1315	arg1	structure					1328:1336	the initial population structure	1305:1336	the initial population structure of the bacterial community at a given location and time	1305:1392	The extent to which individual mechanisms prevail depended on the initial population structure of the bacterial community at a given location and time, as well as the growth rate of specific bacteria.
31602876	0	0	from	evaluation	14:23	arg1	area					81:84	Santai area]	74:85	Santai area]	74:85	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	4	1	theme	high	766:769	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	1	theme	high	766:769	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	1	theme	high	766:769	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	1	theme	high	766:769	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	1	theme	high	766:769	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	1	2	theme	medicines	137:145	arg1	medicines					137:145	the commonly used medicines	119:145	the commonly used medicines	119:145	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	1	2	theme	medicines	137:145	arg1	one					112:114	one	112:114	one	112:114	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	1	3	theme	health	213:218	arg1	care					220:223	people's daily health care	198:223	people's daily health care	198:223	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	0	4	theme	Santai	74:79	arg1	area					81:84	Santai area]	74:85	Santai area]	74:85	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	3	5	theme	food	442:445	arg1	materials					451:459	the new food raw materials	434:459	the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus)	434:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	4	6	theme	new	827:829	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	6	theme	new	827:829	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	6	theme	new	827:829	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	6	theme	new	827:829	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	6	theme	new	827:829	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	0	7	from	[Analysis	0:8	arg1	area					81:84	Santai area]	74:85	Santai area]	74:85	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	2	8	from	herbs	280:284	arg1	province					297:304	Sichuan province	289:304	Sichuan province	289:304	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	3	9	theme	geographical	490:501	arg1	japonicus					536:544	Fu Cheng O. japonicus	524:544	Fu Cheng O. japonicus	524:544	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	9	theme	geographical	490:501	arg1	products					514:521	the geographical indication products	486:521	the geographical indication products( Fu Cheng O. japonicus)	486:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	2	10	theme	Sichuan	242:248	arg1	province					250:257	Sichuan province	242:257	Sichuan province	242:257	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	4	11	theme	raw	836:838	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	11	theme	raw	836:838	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	11	theme	raw	836:838	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	11	theme	raw	836:838	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	11	theme	raw	836:838	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	3	12	theme	unique	373:378	arg1	advantage					393:401	the unique geographical advantage	369:401	the unique geographical advantage	369:401	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	4	13	theme	food	831:834	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	13	theme	food	831:834	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	13	theme	food	831:834	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	13	theme	food	831:834	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	13	theme	food	831:834	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	3	14	theme	O.	620:621	arg1	components					606:615	the nutritional components	590:615	the nutritional components of O. japonicus in Santai county	590:648	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	15	theme	O.	464:465	arg1	materials					451:459	the new food raw materials	434:459	the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus)	434:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	16	theme	geographical	380:391	arg1	advantage					393:401	the unique geographical advantage	369:401	the unique geographical advantage	369:401	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	2	17	theme	Dao-di	273:278	arg1	herbs					280:284	the famous Dao-di herbs	262:284	the famous Dao-di herbs in Sichuan province	262:304	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	2	17	theme	Dao-di	273:278	arg1	japonicus					229:237	O. japonicus	226:237	O. japonicus in Sichuan province	226:257	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	4	18	theme	high	746:749	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	18	theme	high	746:749	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	18	theme	high	746:749	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	18	theme	high	746:749	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	18	theme	high	746:749	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	2	19	theme	famous	266:271	arg1	herbs					280:284	the famous Dao-di herbs	262:284	the famous Dao-di herbs in Sichuan province	262:304	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	2	19	theme	famous	266:271	arg1	japonicus					229:237	O. japonicus	226:237	O. japonicus in Sichuan province	226:257	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	4	20	theme	nutrients	707:715	arg1	content					692:698	the content	688:698	the content of the nutrients	688:715	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	1	21	theme	Ophiopogon	88:97	arg1	japonicus					99:107	Ophiopogon japonicus	88:107	Ophiopogon japonicus	88:107	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	3	22	theme	indication	503:512	arg1	japonicus					536:544	Fu Cheng O. japonicus	524:544	Fu Cheng O. japonicus	524:544	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	22	theme	indication	503:512	arg1	products					514:521	the geographical indication products	486:521	the geographical indication products( Fu Cheng O. japonicus)	486:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	0	23	theme	composition	37:47	arg1	evaluation					14:23	evaluation	14:23	evaluation	14:23	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	0	23	theme	composition	37:47	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis	0:8	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	3	24	dep	O.	464:465	arg1	japonicus					467:475	O. japonicus	464:475	O. japonicus	464:475	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	1	25	theme	therapeutic	177:187	arg1	food					189:192	a therapeutic food	175:192	a therapeutic food for people's daily health care	175:223	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	3	26	theme	Fu	524:525	arg1	japonicus					536:544	Fu Cheng O. japonicus	524:544	Fu Cheng O. japonicus	524:544	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	26	theme	Fu	524:525	arg1	products					514:521	the geographical indication products	486:521	the geographical indication products( Fu Cheng O. japonicus)	486:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	2	27	theme	Sichuan	346:352	arg1	province					354:361	Santai county,Sichuan province	332:361	Santai county,Sichuan province	332:361	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	0	28	theme	nutrient	28:35	arg1	composition					37:47	nutrient composition	28:47	nutrient composition of Ophiopogonis Radix	28:69	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	3	29	dep	O.	620:621	arg1	japonicus					623:631	O. japonicus	620:631	O. japonicus	620:631	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	30	theme	Cheng	527:531	arg1	japonicus					536:544	Fu Cheng O. japonicus	524:544	Fu Cheng O. japonicus	524:544	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	30	theme	Cheng	527:531	arg1	products					514:521	the geographical indication products	486:521	the geographical indication products( Fu Cheng O. japonicus)	486:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	4	31	theme	experimental	655:666	arg1	results					668:674	The experimental results	651:674	The experimental results	651:674	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	0	32	theme	Ophiopogonis	52:63	arg1	Radix					65:69	Ophiopogonis Radix	52:69	Ophiopogonis Radix	52:69	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	3	33	theme	new	438:440	arg1	materials					451:459	the new food raw materials	434:459	the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus)	434:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	4	34	theme	vitamin	790:796	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	34	theme	vitamin	790:796	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	34	theme	vitamin	790:796	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	34	theme	vitamin	790:796	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	34	theme	vitamin	790:796	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	35	theme	low	738:740	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	35	theme	low	738:740	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	35	theme	low	738:740	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	35	theme	low	738:740	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	35	theme	low	738:740	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	3	36	theme	nutritional	594:604	arg1	components					606:615	the nutritional components	590:615	the nutritional components of O. japonicus in Santai county	590:648	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	4	37	theme	high	785:788	arg1	B2					798:799	high vitamin B2	785:799	high vitamin B2	785:799	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	37	theme	high	785:788	arg1	fat					742:744	low fat	738:744	low fat	738:744	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	37	theme	high	785:788	arg1	polysaccharide					751:764	high polysaccharide	746:764	high polysaccharide	746:764	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	37	theme	high	785:788	arg1	potassium					771:779	high potassium	766:779	high potassium	766:779	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	4	37	theme	high	785:788	arg1	materials					840:848	new food raw materials	827:848	new food raw materials	827:848	The experimental results showed that the content of the nutrients was characterized by low fat,high polysaccharide,high potassium and high vitamin B2,which can be developed as new food raw materials.
31602876	2	38	theme	Sichuan	289:295	arg1	province					297:304	Sichuan province	289:304	Sichuan province	289:304	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	2	39	theme	O.	226:227	arg1	japonicus					229:237	O. japonicus	226:237	O. japonicus in Sichuan province	226:257	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	2	39	theme	O.	226:227	arg1	herbs					280:284	the famous Dao-di herbs	262:284	the famous Dao-di herbs in Sichuan province	262:304	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	3	40	from	components	606:615	arg1	county					643:648	county	643:648	county	643:648	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	2	41	from	japonicus	229:237	arg1	province					250:257	Sichuan province	242:257	Sichuan province	242:257	O. japonicus in Sichuan province is the famous Dao-di herbs in Sichuan province,and is mainly produced in Santai county,Sichuan province.
31602876	3	42	theme	O.	533:534	arg1	japonicus					536:544	Fu Cheng O. japonicus	524:544	Fu Cheng O. japonicus	524:544	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	42	theme	O.	533:534	arg1	products					514:521	the geographical indication products	486:521	the geographical indication products( Fu Cheng O. japonicus)	486:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	3	43	theme	raw	447:449	arg1	materials					451:459	the new food raw materials	434:459	the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus)	434:545	With the unique geographical advantage,Santai county plans to declare the new food raw materials of O. japonicus based on the geographical indication products( Fu Cheng O. japonicus),so it is necessary to analyze and evaluate the nutritional components of O. japonicus in Santai county.
31602876	0	44	theme	Radix	65:69	arg1	composition					37:47	nutrient composition	28:47	nutrient composition of Ophiopogonis Radix	28:69	[Analysis and evaluation of nutrient composition of Ophiopogonis Radix in Santai area].
31602876	1	45	theme	used	132:135	arg1	medicines					137:145	the commonly used medicines	119:145	the commonly used medicines	119:145	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
31602876	1	46	theme	daily	207:211	arg1	care					220:223	people's daily health care	198:223	people's daily health care	198:223	Ophiopogon japonicus is one of the commonly used medicines,and it has gradually become a therapeutic food for people's daily health care.
29909036	0	0	theme	functional	81:90	arg1	properties					92:101	functional properties	81:101	functional properties	81:101	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	7	1	theme	hydrocolloid	1009:1020	arg1	CMM					968:970	CMM	968:970	CMM	968:970	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	7	1	theme	hydrocolloid	1009:1020	arg1	source					999:1004	a new source	993:1004	a new source of hydrocolloid with high-temperature stability	993:1052	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	2	2	theme	CMM	357:359	arg1	extraction					361:370	CMM extraction	357:370	CMM extraction	357:370	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	1	3	theme	myxa	260:263	arg1	mucilage					265:272	Cordia myxa mucilage	253:272	Cordia myxa mucilage (CMM)	253:278	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	3	theme	myxa	260:263	arg1	CMM					275:277	CMM	275:277	CMM	275:277	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	5	4	contain	containing	760:769	arg1	CMM					715:717	CMM	715:717	CMM	715:717	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	5	4	contain	containing	760:769	arg1	polysaccharide					745:758	a low molecular weight polysaccharide	722:758	a low molecular weight polysaccharide containing three fractions with various molecular weights	722:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	5	4	contain	containing	760:769	arg2	fractions					777:785	three fractions	771:785	three fractions	771:785	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	1	5	theme	chemical	159:166	arg1	composition					168:178	chemical composition	159:178	chemical composition	159:178	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	6	theme	mucilage	265:272	arg1	composition					168:178	chemical composition	159:178	chemical composition	159:178	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	6	theme	mucilage	265:272	arg1	behavior					193:200	rheological behavior	181:200	rheological behavior	181:200	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	6	theme	mucilage	265:272	arg1	activity					215:222	antioxidant activity	203:222	antioxidant activity	203:222	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	6	theme	mucilage	265:272	arg1	properties					239:248	functional properties	228:248	functional properties	228:248	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	3	7	contain	had	495:497	arg1	CMM					491:493	CMM	491:493	CMM	491:493	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	7	contain	had	495:497	arg2	protein					556:562	8.90% protein	550:562	8.90% protein	550:562	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	7	contain	had	495:497	arg2	fat					591:593	1.00% fat	585:593	1.00% fat	585:593	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	7	contain	had	495:497	arg2	carbohydrate					519:530	77.51% carbohydrate	512:530	77.51% carbohydrate	512:530	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	7	contain	had	495:497	arg2	ash					545:547	5.86% total ash	533:547	5.86% total ash	533:547	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	7	contain	had	495:497	arg2	moisture					571:578	6.90% moisture	565:578	6.90% moisture	565:578	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	2	8	theme	surface	290:296	arg1	RSM					311:313	RSM	311:313	RSM	311:313	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	2	8	theme	surface	290:296	arg1	methodology					298:308	Response surface methodology	281:308	Response surface methodology (RSM)	281:314	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	3	9	theme	77.51	512:516	arg1	%					517:517	%	517:517	%	517:517	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	2	10	theme	Response	281:288	arg1	RSM					311:313	RSM	311:313	RSM	311:313	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	2	10	theme	Response	281:288	arg1	methodology					298:308	Response surface methodology	281:308	Response surface methodology (RSM)	281:314	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	0	11	theme	myxa	113:116	arg1	mucilage					118:125	Cordia myxa mucilage	106:125	Cordia myxa mucilage	106:125	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	8	12	theme	antiradical	1077:1087	arg1	capacity					1089:1096	the desirable antiradical capacity	1063:1096	the desirable antiradical capacity	1063:1096	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	1	13	theme	antioxidant	203:213	arg1	activity					215:222	antioxidant activity	203:222	antioxidant activity	203:222	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	8	14	contain	had	1059:1061	arg2	capacity					1138:1145	water/oil holding capacity	1120:1145	water/oil holding capacity	1120:1145	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	8	14	contain	had	1059:1061	arg2	capacity					1089:1096	the desirable antiradical capacity	1063:1096	the desirable antiradical capacity	1063:1096	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	8	14	contain	had	1059:1061	arg1	CMM					1055:1057	CMM	1055:1057	CMM	1055:1057	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	8	14	contain	had	1059:1061	arg2	solubility					1105:1114	water solubility	1099:1114	water solubility	1099:1114	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	8	15	theme	water	1099:1103	arg1	solubility					1105:1114	water solubility	1099:1114	water solubility	1099:1114	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	2	16	theme	ultrasound	388:397	arg1	power					399:403	ultrasound power	388:403	ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w	388:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	0	17	theme	Cordia	106:111	arg1	mucilage					118:125	Cordia myxa mucilage	106:125	Cordia myxa mucilage	106:125	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	2	18	theme	water	465:469	arg1	ratio					471:475	water ratio	465:475	water ratio of 16.25 w/w	465:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	5	19	theme	weight	738:743	arg1	CMM					715:717	CMM	715:717	CMM	715:717	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	5	19	theme	weight	738:743	arg1	polysaccharide					745:758	a low molecular weight polysaccharide	722:758	a low molecular weight polysaccharide containing three fractions with various molecular weights	722:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	2	20	theme	88.05 °C	443:450	arg1	99.37 W					408:414	99.37 W	408:414	99.37 W	408:414	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	2	20	theme	88.05 °C	443:450	arg1	temperature					428:438	extraction temperature	417:438	extraction temperature of 88.05 °C	417:450	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	8	21	theme	desirable	1067:1075	arg1	capacity					1089:1096	the desirable antiradical capacity	1063:1096	the desirable antiradical capacity	1063:1096	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	1	22	theme	rheological	181:191	arg1	behavior					193:200	rheological behavior	181:200	rheological behavior	181:200	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	6	23	theme	typical	872:878	arg1	bands					880:884	all typical bands	868:884	all typical bands	868:884	FT-IR spectrum illustrated that this polymer had all typical bands and peaks characteristics of polysaccharides.
29909036	8	24	theme	water/oil	1120:1128	arg1	capacity					1138:1145	water/oil holding capacity	1120:1145	water/oil holding capacity	1120:1145	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	3	25	theme	5.86	533:536	arg1	ash					545:547	5.86% total ash	533:547	5.86% total ash	533:547	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	4	26	dep	added	662:666	arg1	value					656:660	value	656:660	value	656:660	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	2	27	theme	temperature	428:438	arg1	power					399:403	ultrasound power	388:403	ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w	388:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	4	28	theme	food	682:685	arg1	systems					706:712	food and pharmaceutical systems	682:712	food and pharmaceutical systems	682:712	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	3	29	theme	%	517:517	arg1	carbohydrate					519:530	77.51% carbohydrate	512:530	77.51% carbohydrate	512:530	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	7	30	theme	new	995:997	arg1	CMM					968:970	CMM	968:970	CMM	968:970	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	7	30	theme	new	995:997	arg1	source					999:1004	a new source	993:1004	a new source of hydrocolloid with high-temperature stability	993:1052	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	2	31	theme	extraction	417:426	arg1	temperature					428:438	extraction temperature	417:438	extraction temperature of 88.05 °C	417:450	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	0	32	theme	mucilage	118:125	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	0	32	theme	mucilage	118:125	arg1	composition					21:31	chemical composition	12:31	chemical composition	12:31	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	0	32	theme	mucilage	118:125	arg1	behavior					46:53	rheological behavior	34:53	rheological behavior	34:53	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	0	32	theme	mucilage	118:125	arg1	activity					68:75	antioxidant activity	56:75	antioxidant activity	56:75	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	0	32	theme	mucilage	118:125	arg1	properties					92:101	functional properties	81:101	functional properties	81:101	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	5	33	theme	various	792:798	arg1	weights					810:816	various molecular weights	792:816	various molecular weights	792:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	2	34	theme	99.37 W	408:414	arg1	power					399:403	ultrasound power	388:403	ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w	388:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	0	35	theme	rheological	34:44	arg1	behavior					46:53	rheological behavior	34:53	rheological behavior	34:53	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	4	36	theme	high	605:608	arg1	level					610:614	a high level	603:614	a high level of nutrients	603:627	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	4	37	theme	pharmaceutical	691:704	arg1	systems					706:712	food and pharmaceutical systems	682:712	food and pharmaceutical systems	682:712	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	7	38	theme	high-temperature	1027:1042	arg1	stability					1044:1052	high-temperature stability	1027:1052	high-temperature stability	1027:1052	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	5	39	theme	molecular	728:736	arg1	CMM					715:717	CMM	715:717	CMM	715:717	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	5	39	theme	molecular	728:736	arg1	polysaccharide					745:758	a low molecular weight polysaccharide	722:758	a low molecular weight polysaccharide containing three fractions with various molecular weights	722:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	3	40	theme	6.90	565:568	arg1	%					569:569	%	569:569	%	569:569	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	6	41	contain	had	864:866	arg2	bands					880:884	all typical bands	868:884	all typical bands	868:884	FT-IR spectrum illustrated that this polymer had all typical bands and peaks characteristics of polysaccharides.
29909036	6	41	contain	had	864:866	arg1	polymer					856:862	this polymer	851:862	this polymer	851:862	FT-IR spectrum illustrated that this polymer had all typical bands and peaks characteristics of polysaccharides.
29909036	5	42	theme	low	724:726	arg1	CMM					715:717	CMM	715:717	CMM	715:717	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	5	42	theme	low	724:726	arg1	polysaccharide					745:758	a low molecular weight polysaccharide	722:758	a low molecular weight polysaccharide containing three fractions with various molecular weights	722:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	2	43	theme	solid	456:460	arg1	power					399:403	ultrasound power	388:403	ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w	388:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	7	44	theme	shear	948:952	arg1	measurements					954:965	steady shear measurements	941:965	steady shear measurements	941:965	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	2	45	theme	16.25	480:484	arg1	ratio					471:475	water ratio	465:475	water ratio of 16.25 w/w	465:488	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	3	46	theme	%	537:537	arg1	ash					545:547	5.86% total ash	533:547	5.86% total ash	533:547	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	5	47	theme	molecular	800:808	arg1	weights					810:816	various molecular weights	792:816	various molecular weights	792:816	CMM is a low molecular weight polysaccharide containing three fractions with various molecular weights.
29909036	8	48	theme	holding	1130:1136	arg1	capacity					1138:1145	water/oil holding capacity	1120:1145	water/oil holding capacity	1120:1145	CMM had the desirable antiradical capacity, water solubility and water/oil holding capacity.
29909036	3	49	theme	%	589:589	arg1	fat					591:593	1.00% fat	585:593	1.00% fat	585:593	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	50	theme	total	539:543	arg1	ash					545:547	5.86% total ash	533:547	5.86% total ash	533:547	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	2	51	theme	optimum	334:340	arg1	conditions					342:351	optimum conditions	334:351	optimum conditions for CMM extraction	334:370	Response surface methodology (RSM) demonstrated that optimum conditions for CMM extraction were as follow: ultrasound power of 99.37 W, extraction temperature of 88.05 °C and solid to water ratio of 16.25 w/w.
29909036	3	52	theme	%	554:554	arg1	protein					556:562	8.90% protein	550:562	8.90% protein	550:562	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	53	theme	%	569:569	arg1	moisture					571:578	6.90% moisture	565:578	6.90% moisture	565:578	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	1	54	theme	functional	228:237	arg1	properties					239:248	functional properties	228:248	functional properties	228:248	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	7	55	theme	steady	941:946	arg1	measurements					954:965	steady shear measurements	941:965	steady shear measurements	941:965	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	4	56	theme	added	662:666	arg1	by-product					668:677	a value added by-product	654:677	a value added by-product in food and pharmaceutical systems	654:712	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	4	56	theme	added	662:666	arg1	CMM					630:632	CMM	630:632	CMM	630:632	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	4	57	theme	nutrients	619:627	arg1	level					610:614	a high level	603:614	a high level of nutrients	603:627	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	3	58	theme	1.00	585:588	arg1	%					589:589	%	589:589	%	589:589	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	3	59	theme	8.90	550:553	arg1	%					554:554	%	554:554	%	554:554	CMM had, on average, 77.51% carbohydrate, 5.86% total ash, 8.90% protein, 6.90% moisture, and 1.00% fat.
29909036	7	60	with	source	999:1004	arg1	stability					1044:1052	high-temperature stability	1027:1052	high-temperature stability	1027:1052	Based on steady shear measurements, CMM can be introduced as a new source of hydrocolloid with high-temperature stability.
29909036	6	61	theme	polysaccharides	915:929	arg1	characteristics					896:910	characteristics	896:910	characteristics of polysaccharides	896:929	FT-IR spectrum illustrated that this polymer had all typical bands and peaks characteristics of polysaccharides.
29909036	0	62	theme	antioxidant	56:66	arg1	activity					68:75	antioxidant activity	56:75	antioxidant activity	56:75	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	6	63	theme	FT-IR	819:823	arg1	spectrum					825:832	FT-IR spectrum	819:832	FT-IR spectrum	819:832	FT-IR spectrum illustrated that this polymer had all typical bands and peaks characteristics of polysaccharides.
29909036	0	64	theme	chemical	12:19	arg1	composition					21:31	chemical composition	12:31	chemical composition	12:31	Extraction, chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage.
29909036	4	65	from	by-product	668:677	arg1	systems					706:712	food and pharmaceutical systems	682:712	food and pharmaceutical systems	682:712	Due to a high level of nutrients, CMM can be suggested as a value added by-product in food and pharmaceutical systems.
29909036	1	66	theme	Cordia	253:258	arg1	mucilage					265:272	Cordia myxa mucilage	253:272	Cordia myxa mucilage (CMM)	253:278	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
29909036	1	66	theme	Cordia	253:258	arg1	CMM					275:277	CMM	275:277	CMM	275:277	This paper aims to investigate chemical composition, rheological behavior, antioxidant activity and functional properties of Cordia myxa mucilage (CMM).
30989896	5	0	from	effects	923:929	arg1	mice					946:949	AAD mice	942:949	AAD mice	942:949	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	1	1	theme	associated	255:264	arg1	diarrhea					266:273	antibiotic associated diarrhea	244:273	antibiotic associated diarrhea( AAD) in mice	244:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	1	theme	associated	255:264	arg1	AAD					276:278	AAD	276:278	AAD	276:278	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	0	2	theme	diarrhea	86:93	arg1	mice					95:98	antibiotic-associated diarrhea mice	64:98	antibiotic-associated diarrhea mice	64:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	3	3	theme	WGPN	646:649	arg1	saline					602:607	normal saline	595:607	normal saline( natural recovery group,NR)	595:635	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	3	3	theme	WGPN	646:649	arg1	group					651:655	WGPN group	646:655	WGPN group	646:655	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	1	4	theme	gut	165:167	arg1	composition					180:190	gut microbiota composition	165:190	gut microbiota composition	165:190	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	8	5	theme	ileum	1403:1407	arg1	structure					1409:1417	the ileum structure	1399:1417	the ileum structure	1399:1417	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	0	6	from	[Effects	0:7	arg1	microbiota					50:59	gut microbiota	46:59	gut microbiota in antibiotic-associated diarrhea mice	46:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	7	7	theme	Streptococcus	1268:1280	arg1	abundance					1243:1251	the relative abundance	1230:1251	the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level	1230:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	6	8	from	symptoms	1001:1008	arg1	mice					1025:1028	mice	1025:1028	mice	1025:1028	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	3	9	theme	gastric	520:526	arg1	gavage					528:533	gastric gavage	520:533	gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week	520:669	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	5	10	theme	WGPN	934:937	arg1	effects					923:929	the effects	919:929	the effects of WGPN on AAD mice	919:949	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	5	11	theme	intestinal	872:881	arg1	contents					883:890	intestinal contents	872:890	intestinal contents	872:890	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	0	12	from	microbiota	50:59	arg1	mice					95:98	antibiotic-associated diarrhea mice	64:98	antibiotic-associated diarrhea mice	64:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	5	13	dep	h	753:753	arg1	administration					770:783	the last administration	761:783	the last administration	761:783	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	6	14	theme	villi	1113:1117	arg1	length					1092:1097	the length	1088:1097	the length of intestinal villi	1088:1117	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	8	15	from	composition	1451:1461	arg1	mice					1492:1495	AAD mice	1488:1495	AAD mice	1488:1495	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	7	16	theme	relative	1167:1174	arg1	abundance					1176:1184	the relative abundance	1163:1184	the relative abundance of Lactobacillus	1163:1201	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	2	17	theme	DEAE-sepharose	411:424	arg1	column					436:441	DEAE-sepharose fast flow column	411:441	DEAE-sepharose fast flow column	411:441	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	1	18	theme	microbiota	169:178	arg1	composition					180:190	gut microbiota composition	165:190	gut microbiota composition	165:190	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	3	19	with	gavage	528:533	arg1	hydrochloride					551:563	lincomycin hydrochloride	540:563	lincomycin hydrochloride	540:563	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	5	20	theme	high	833:836	arg1	analysis					860:867	high throughput sequencing analysis	833:867	high throughput sequencing analysis	833:867	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	8	21	from	diversity	1437:1445	arg1	mice					1492:1495	AAD mice	1488:1495	AAD mice	1488:1495	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	6	22	theme	diarrhea	1013:1020	arg1	symptoms					1001:1008	the symptoms	997:1008	the symptoms of diarrhea in mice	997:1028	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	7	23	theme	relative	1234:1241	arg1	abundance					1243:1251	the relative abundance	1230:1251	the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level	1230:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	0	24	theme	antibiotic-associated	64:84	arg1	mice					95:98	antibiotic-associated diarrhea mice	64:98	antibiotic-associated diarrhea mice	64:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	2	25	theme	neutral	316:322	arg1	WGPN					340:343	WGPN	340:343	WGPN	340:343	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	25	theme	neutral	316:322	arg1	polysaccharide					324:337	The water-soluble ginseng neutral polysaccharide	290:337	The water-soluble ginseng neutral polysaccharide( WGPN)	290:344	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	5	26	theme	ileum	813:817	arg1	rRNA					828:831	16 S rRNA	823:831	16 S rRNA	823:831	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	5	26	theme	ileum	813:817	arg1	observation					798:808	histological observation	785:808	histological observation of ileum	785:817	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	6	27	theme	intestinal	1102:1111	arg1	villi					1113:1117	intestinal villi	1102:1117	intestinal villi	1102:1117	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	8	28	theme	gut	1470:1472	arg1	microbiota					1474:1483	the gut microbiota	1466:1483	the gut microbiota	1466:1483	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	2	29	theme	ginseng	308:314	arg1	WGPN					340:343	WGPN	340:343	WGPN	340:343	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	29	theme	ginseng	308:314	arg1	polysaccharide					324:337	The water-soluble ginseng neutral polysaccharide	290:337	The water-soluble ginseng neutral polysaccharide( WGPN)	290:344	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	4	30	theme	diarrhea	706:713	arg1	status					715:720	diarrhea status	706:720	diarrhea status	706:720	Body weight changes,psychosis and diarrhea status were observed and assessed.
30989896	2	31	theme	ginseng	378:384	arg1	WGP					403:405	WGP	403:405	WGP	403:405	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	31	theme	ginseng	378:384	arg1	polysaccharides					386:400	water-soluble ginseng polysaccharides	364:400	water-soluble ginseng polysaccharides( WGP)	364:406	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	0	32	theme	neutral	20:26	arg1	polysaccharide					28:41	ginseng neutral polysaccharide	12:41	ginseng neutral polysaccharide	12:41	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	4	33	theme	weight	677:682	arg1	changes					684:690	Body weight changes	672:690	Body weight changes	672:690	Body weight changes,psychosis and diarrhea status were observed and assessed.
30989896	1	34	from	effect	121:126	arg1	diversity					196:204	diversity	196:204	diversity	196:204	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	34	from	effect	121:126	arg1	composition					180:190	gut microbiota composition	165:190	gut microbiota composition	165:190	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	2	35	theme	water-soluble	294:306	arg1	WGPN					340:343	WGPN	340:343	WGPN	340:343	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	35	theme	water-soluble	294:306	arg1	polysaccharide					324:337	The water-soluble ginseng neutral polysaccharide	290:337	The water-soluble ginseng neutral polysaccharide( WGPN)	290:344	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	36	theme	fast	426:429	arg1	column					436:441	DEAE-sepharose fast flow column	411:441	DEAE-sepharose fast flow column	411:441	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	37	theme	flow	431:434	arg1	column					436:441	DEAE-sepharose fast flow column	411:441	DEAE-sepharose fast flow column	411:441	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	5	38	theme	contents	883:890	arg1	rRNA					828:831	16 S rRNA	823:831	16 S rRNA	823:831	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	5	38	theme	contents	883:890	arg1	observation					798:808	histological observation	785:808	histological observation of ileum	785:817	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	3	39	theme	lincomycin	540:549	arg1	hydrochloride					551:563	lincomycin hydrochloride	540:563	lincomycin hydrochloride	540:563	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	2	40	theme	water-soluble	364:376	arg1	WGP					403:405	WGP	403:405	WGP	403:405	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	2	40	theme	water-soluble	364:376	arg1	polysaccharides					386:400	water-soluble ginseng polysaccharides	364:400	water-soluble ginseng polysaccharides( WGP)	364:406	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	7	41	theme	Lactobacillus	1189:1201	arg1	abundance					1176:1184	the relative abundance	1163:1184	the relative abundance of Lactobacillus	1163:1201	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	5	42	theme	AAD	942:944	arg1	mice					946:949	AAD mice	942:949	AAD mice	942:949	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	7	43	theme	Ochrobactrum	1282:1293	arg1	abundance					1243:1251	the relative abundance	1230:1251	the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level	1230:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	3	44	dep	saline	602:607	arg1	NR					633:634	NR	633:634	NR	633:634	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	3	44	dep	saline	602:607	arg1	group					627:631	natural recovery group	610:631	natural recovery group	610:631	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	0	45	theme	polysaccharide	28:41	arg1	[Effects					0:7	[Effects	0:7	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice	0:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	8	46	theme	microbiota	1474:1483	arg1	composition					1451:1461	composition	1451:1461	composition	1451:1461	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	8	46	theme	microbiota	1474:1483	arg1	diversity					1437:1445	diversity	1437:1445	diversity	1437:1445	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	1	47	from	diarrhea	266:273	arg1	mice					284:287	mice	284:287	mice	284:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	3	48	theme	recovery	618:625	arg1	NR					633:634	NR	633:634	NR	633:634	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	3	48	theme	recovery	618:625	arg1	group					627:631	natural recovery group	610:631	natural recovery group	610:631	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	5	49	theme	histological	785:796	arg1	observation					798:808	histological observation	785:808	histological observation of ileum	785:817	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	8	50	theme	gut	1368:1370	arg1	microecology					1372:1383	the gut microecology	1364:1383	the gut microecology	1364:1383	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	5	51	theme	16	823:824	arg1	S					826:826	S	826:826	S	826:826	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	7	52	theme	NR	1135:1136	arg1	mice					1138:1141	NR mice	1135:1141	NR mice	1135:1141	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	5	53	dep	observation	798:808	arg1	analysis					860:867	high throughput sequencing analysis	833:867	high throughput sequencing analysis	833:867	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	5	54	theme	throughput	838:847	arg1	analysis					860:867	high throughput sequencing analysis	833:867	high throughput sequencing analysis	833:867	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	0	55	theme	gut	46:48	arg1	microbiota					50:59	gut microbiota	46:59	gut microbiota in antibiotic-associated diarrhea mice	46:98	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	8	56	theme	AAD	1488:1490	arg1	mice					1492:1495	AAD mice	1488:1495	AAD mice	1488:1495	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	3	57	theme	WGPN	640:643	arg1	administration					577:590	administration	577:590	administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week	577:669	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	3	58	theme	AAD	495:497	arg1	mice					499:502	AAD mice	495:502	AAD mice	495:502	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	5	59	theme	sequencing	849:858	arg1	analysis					860:867	high throughput sequencing analysis	833:867	high throughput sequencing analysis	833:867	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	3	60	theme	saline	602:607	arg1	administration					577:590	administration	577:590	administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week	577:669	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	8	61	dep	diversity	1437:1445	arg1	the					1433:1435	the	1433:1435	the	1433:1435	In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
30989896	4	62	theme	Body	672:675	arg1	changes					684:690	Body weight changes	672:690	Body weight changes	672:690	Body weight changes,psychosis and diarrhea status were observed and assessed.
30989896	7	63	theme	Pseudomonas	1299:1309	arg1	abundance					1243:1251	the relative abundance	1230:1251	the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level	1230:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	1	64	theme	therapeutic	221:231	arg1	effect					233:238	the therapeutic effect	217:238	the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice	117:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	65	from	effect	233:238	arg1	diversity					196:204	diversity	196:204	diversity	196:204	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	65	from	effect	233:238	arg1	composition					180:190	gut microbiota composition	165:190	gut microbiota composition	165:190	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	3	66	theme	natural	610:616	arg1	recovery					618:625	natural recovery	610:625	natural recovery group	610:631	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	7	67	theme	genus	1318:1322	arg1	level					1324:1328	the genus level	1314:1328	the genus level	1314:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	1	68	theme	ginseng	131:137	arg1	polysaccharide					147:160	ginseng neutral polysaccharide	131:160	ginseng neutral polysaccharide	131:160	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	2	69	theme	ginseng	486:492	arg1	roots					471:475	the roots	467:475	the roots of Panax ginseng	467:492	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	5	70	theme	S	826:826	arg1	rRNA					828:831	16 S rRNA	823:831	16 S rRNA	823:831	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	3	71	theme	normal	595:600	arg1	saline					602:607	normal saline	595:607	normal saline( natural recovery group,NR)	595:635	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	3	71	theme	normal	595:600	arg1	group					651:655	WGPN group	646:655	WGPN group	646:655	AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week.
30989896	5	72	theme	last	765:768	arg1	administration					770:783	the last administration	761:783	the last administration	761:783	12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice.
30989896	1	73	theme	neutral	139:145	arg1	polysaccharide					147:160	ginseng neutral polysaccharide	131:160	ginseng neutral polysaccharide	131:160	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	6	74	theme	ileum	1069:1073	arg1	edema					1060:1064	edema	1060:1064	edema	1060:1064	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	6	74	theme	ileum	1069:1073	arg1	inflammation					1043:1054	inflammation	1043:1054	inflammation	1043:1054	The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi.
30989896	2	75	theme	Panax	480:484	arg1	ginseng					486:492	Panax ginseng	480:492	Panax ginseng	480:492	The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng.
30989896	0	76	theme	ginseng	12:18	arg1	polysaccharide					28:41	ginseng neutral polysaccharide	12:41	ginseng neutral polysaccharide	12:41	[Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice].
30989896	1	77	theme	polysaccharide	147:160	arg1	effect					121:126	the effect	117:126	the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice	117:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	77	theme	polysaccharide	147:160	arg1	effect					233:238	the therapeutic effect	217:238	the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice	117:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	7	78	from	level	1324:1328	arg1	abundance					1243:1251	the relative abundance	1230:1251	the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level	1230:1328	As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level.
30989896	1	79	theme	antibiotic	244:253	arg1	diarrhea					266:273	antibiotic associated diarrhea	244:273	antibiotic associated diarrhea( AAD) in mice	244:287	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
30989896	1	79	theme	antibiotic	244:253	arg1	AAD					276:278	AAD	276:278	AAD	276:278	To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice.
29408356	8	0	theme	tensile	1183:1189	arg1	lower					1243:1247	lower	1243:1247	lower	1243:1247	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	8	0	theme	tensile	1183:1189	arg1	strength					1191:1198	The tensile strength	1179:1198	The tensile strength of the all-cellulose composites	1179:1230	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	8	0	theme	tensile	1183:1189	arg1	higher					1297:1302	higher	1297:1302	higher	1297:1302	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	7	1	theme	composites	1070:1079	arg1	stability					1039:1047	The thermal stability	1027:1047	The thermal stability of the all-cellulose composites	1027:1079	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	7	1	theme	composites	1070:1079	arg1	higher					1097:1102	higher	1097:1102	higher	1097:1102	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	8	2	theme	cellulose	1266:1274	arg1	matrix					1276:1281	the cellulose matrix	1262:1281	the cellulose matrix	1262:1281	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	6	3	theme	composites	959:968	arg1	lower					986:990	lower	986:990	lower	986:990	The crystallinity of the all-cellulose composites was found to be lower than that of the cellulose matrix.
29408356	6	3	theme	composites	959:968	arg1	crystallinity					924:936	The crystallinity	920:936	The crystallinity of the all-cellulose composites	920:968	The crystallinity of the all-cellulose composites was found to be lower than that of the cellulose matrix.
29408356	0	4	theme	fibril	79:84	arg1	fillers					86:92	Napier grass cellulose fibril fillers	56:92	Napier grass cellulose fibril fillers	56:92	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	9	5	theme	mulching	1397:1404	arg1	applications					1406:1417	wrapping and mulching applications	1384:1417	wrapping and mulching applications	1384:1417	The all-cellulose composites can be considered for wrapping and mulching applications.
29408356	2	6	used	used	315:318	arg2	CFs					250:252	CFs	250:252	CFs	250:252	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	2	6	used	used	315:318	arg2	fibrils					241:247	cellulose fibrils	231:247	cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres	231:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	2	6	used	used	315:318	arg2	fillers					323:329	fillers	323:329	fillers	323:329	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	1	7	dep	Diverse	95:101	arg1	move					103:106	move	103:106	move	103:106	Diverse move has been attempted to use biomass as a filler for the production of biodegradable all-cellulose composites.
29408356	6	8	theme	all-cellulose	945:957	arg1	composites					959:968	the all-cellulose composites	941:968	the all-cellulose composites	941:968	The crystallinity of the all-cellulose composites was found to be lower than that of the cellulose matrix.
29408356	3	9	from	%	459:459	arg1	varied					437:442	varied	437:442	varied	437:442	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	2	10	theme	native	270:275	arg1	fibres					303:308	native African Napier grass (NG) fibres	270:308	native African Napier grass (NG) fibres	270:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	3	11	from	orientation	491:501	arg1	varied					437:442	varied	437:442	varied	437:442	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	7	12	theme	all-cellulose	1056:1068	arg1	composites					1070:1079	the all-cellulose composites	1052:1079	the all-cellulose composites	1052:1079	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	7	13	theme	cellulose	1121:1129	arg1	matrix					1131:1136	the cellulose matrix	1117:1136	the cellulose matrix	1117:1136	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	6	14	theme	cellulose	1009:1017	arg1	matrix					1019:1024	the cellulose matrix	1005:1024	the cellulose matrix	1005:1024	The crystallinity of the all-cellulose composites was found to be lower than that of the cellulose matrix.
29408356	2	15	theme	made	355:358	arg1	composites					374:383	made all-cellulose composites	355:383	made all-cellulose composites	355:383	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	3	16	from	varied	437:442	arg1	matrix					474:479	cellulose matrix	464:479	cellulose matrix	464:479	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	16	from	varied	437:442	arg1	orientation					491:501	random orientation	484:501	random orientation	484:501	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	4	17	theme	tensile	729:735	arg1	testing					737:743	tensile testing	729:743	tensile testing	729:743	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	5	18	theme	hemicelluloses	816:829	arg1	hemicelluloses					816:829	hemicelluloses	816:829	hemicelluloses	816:829	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	18	theme	hemicelluloses	816:829	arg1	amounts					805:811	minute amounts	798:811	minute amounts of hemicelluloses and lignin	798:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	18	theme	hemicelluloses	816:829	arg1	lignin					835:840	lignin	835:840	lignin	835:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	0	19	theme	composite	14:22	arg1	films					24:28	All-cellulose composite films	0:28	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.	0:93	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	7	20	theme	NGCFs	1157:1161	arg1	content					1170:1176	NGCFs filler content	1157:1176	NGCFs filler content	1157:1176	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	5	21	from	interaction	878:888	arg1	filler					849:854	the filler	845:854	the filler	845:854	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	3	22	dep	25 wt	454:458	arg1	to					451:452	to	451:452	to	451:452	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	23	theme	Grass	393:397	arg1	fibrils					409:415	Napier Grass Cellulose fibrils	386:415	Napier Grass Cellulose fibrils (NGCFs) loading	386:431	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	23	theme	Grass	393:397	arg1	NGCFs					418:422	NGCFs	418:422	NGCFs	418:422	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	0	24	theme	All-cellulose	0:12	arg1	films					24:28	All-cellulose composite films	0:28	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.	0:93	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	7	25	theme	thermal	1031:1037	arg1	stability					1039:1047	The thermal stability	1027:1047	The thermal stability of the all-cellulose composites	1027:1079	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	7	25	theme	thermal	1031:1037	arg1	higher					1097:1102	higher	1097:1102	higher	1097:1102	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	2	26	theme	grass	292:296	arg1	fibres					303:308	native African Napier grass (NG) fibres	270:308	native African Napier grass (NG) fibres	270:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	2	27	theme	cellulose	334:342	arg1	matrix					344:349	cellulose matrix	334:349	cellulose matrix	334:349	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	4	28	dep	transform	618:626	arg1	infrared					628:635	infrared	628:635	transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing	618:743	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	2	29	theme	Napier	285:290	arg1	fibres					303:308	native African Napier grass (NG) fibres	270:308	native African Napier grass (NG) fibres	270:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	1	30	theme	biodegradable	176:188	arg1	composites					204:213	biodegradable all-cellulose composites	176:213	biodegradable all-cellulose composites	176:213	Diverse move has been attempted to use biomass as a filler for the production of biodegradable all-cellulose composites.
29408356	3	31	theme	cellulose	515:523	arg1	composites					525:534	all cellulose composites	511:534	the all cellulose composites	507:534	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	2	32	theme	African	277:283	arg1	fibres					303:308	native African Napier grass (NG) fibres	270:308	native African Napier grass (NG) fibres	270:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	1	33	theme	all-cellulose	190:202	arg1	composites					204:213	biodegradable all-cellulose composites	176:213	biodegradable all-cellulose composites	176:213	Diverse move has been attempted to use biomass as a filler for the production of biodegradable all-cellulose composites.
29408356	5	34	theme	minute	798:803	arg1	hemicelluloses					816:829	hemicelluloses	816:829	hemicelluloses	816:829	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	34	theme	minute	798:803	arg1	amounts					805:811	minute amounts	798:811	minute amounts of hemicelluloses and lignin	798:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	34	theme	minute	798:803	arg1	lignin					835:840	lignin	835:840	lignin	835:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	3	35	theme	Cellulose	399:407	arg1	fibrils					409:415	Napier Grass Cellulose fibrils	386:415	Napier Grass Cellulose fibrils (NGCFs) loading	386:431	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	35	theme	Cellulose	399:407	arg1	NGCFs					418:422	NGCFs	418:422	NGCFs	418:422	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	1	36	theme	composites	204:213	arg1	production					162:171	the production	158:171	the production of biodegradable all-cellulose composites	158:213	Diverse move has been attempted to use biomass as a filler for the production of biodegradable all-cellulose composites.
29408356	0	37	theme	cellulose	35:43	arg1	matrix					45:50	cellulose matrix	35:50	cellulose matrix	35:50	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	3	38	theme	Napier	386:391	arg1	fibrils					409:415	Napier Grass Cellulose fibrils	386:415	Napier Grass Cellulose fibrils (NGCFs) loading	386:431	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	38	theme	Napier	386:391	arg1	NGCFs					418:422	NGCFs	418:422	NGCFs	418:422	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	8	39	theme	composites	1221:1230	arg1	lower					1243:1247	lower	1243:1247	lower	1243:1247	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	8	39	theme	composites	1221:1230	arg1	strength					1191:1198	The tensile strength	1179:1198	The tensile strength of the all-cellulose composites	1179:1230	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	8	39	theme	composites	1221:1230	arg1	higher					1297:1302	higher	1297:1302	higher	1297:1302	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	2	40	theme	all-cellulose	360:372	arg1	composites					374:383	made all-cellulose composites	355:383	made all-cellulose composites	355:383	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	0	41	theme	Napier	56:61	arg1	fillers					86:92	Napier grass cellulose fibril fillers	56:92	Napier grass cellulose fibril fillers	56:92	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	9	42	theme	wrapping	1384:1391	arg1	applications					1406:1417	wrapping and mulching applications	1384:1417	wrapping and mulching applications	1384:1417	The all-cellulose composites can be considered for wrapping and mulching applications.
29408356	3	43	theme	fibrils	409:415	arg1	loading					425:431	Napier Grass Cellulose fibrils (NGCFs) loading	386:431	Napier Grass Cellulose fibrils (NGCFs) loading	386:431	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	0	44	with	films	24:28	arg1	matrix					45:50	cellulose matrix	35:50	cellulose matrix	35:50	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	0	44	with	films	24:28	arg1	fillers					86:92	Napier grass cellulose fibril fillers	56:92	Napier grass cellulose fibril fillers	56:92	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	7	45	theme	filler	1163:1168	arg1	content					1170:1176	NGCFs filler content	1157:1176	NGCFs filler content	1157:1176	The thermal stability of the all-cellulose composites was found to be higher than that of the cellulose matrix and increased with NGCFs filler content.
29408356	5	46	from	presence	786:793	arg1	filler					849:854	the filler	845:854	the filler	845:854	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	4	47	theme	X-ray	658:662	arg1	diffraction					664:674	X-ray diffraction	658:674	X-ray diffraction	658:674	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	4	48	dep	Fourier	610:616	arg1	transform					618:626	transform	618:626	transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing	618:743	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	5	49	attach	presence	786:793	arg1	filler					849:854	the filler	845:854	the filler	845:854	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	49	attach	presence	786:793	arg2	lignin					835:840	lignin	835:840	lignin	835:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	49	attach	presence	786:793	arg2	hemicelluloses					816:829	hemicelluloses	816:829	hemicelluloses	816:829	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	49	attach	presence	786:793	arg2	amounts					805:811	minute amounts	798:811	minute amounts of hemicelluloses and lignin	798:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	4	50	theme	thermogravimetric	677:693	arg1	analysis					695:702	thermogravimetric analysis	677:702	thermogravimetric analysis	677:702	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	5	51	theme	lignin	835:840	arg1	hemicelluloses					816:829	hemicelluloses	816:829	hemicelluloses	816:829	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	51	theme	lignin	835:840	arg1	amounts					805:811	minute amounts	798:811	minute amounts of hemicelluloses and lignin	798:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	51	theme	lignin	835:840	arg1	lignin					835:840	lignin	835:840	lignin	835:840	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	8	52	theme	all-cellulose	1207:1219	arg1	composites					1221:1230	the all-cellulose composites	1203:1230	the all-cellulose composites	1203:1230	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	3	53	theme	random	484:489	arg1	orientation					491:501	random orientation	484:501	random orientation	484:501	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	4	54	theme	optical	705:711	arg1	microscopy					713:722	optical microscopy	705:722	optical microscopy	705:722	These composites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, thermogravimetric analysis, optical microscopy, and tensile testing.
29408356	2	55	theme	NG	299:300	arg1	fibres					303:308	native African Napier grass (NG) fibres	270:308	native African Napier grass (NG) fibres	270:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	5	56	theme	possible	869:876	arg1	interaction					878:888	the possible interaction	865:888	not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs	773:917	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	0	57	theme	cellulose	69:77	arg1	fillers					86:92	Napier grass cellulose fibril fillers	56:92	Napier grass cellulose fibril fillers	56:92	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	5	58	theme	amounts	805:811	arg1	presence					786:793	the presence	782:793	not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs	773:917	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	5	58	theme	amounts	805:811	arg1	interaction					878:888	the possible interaction	865:888	not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs	773:917	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	3	59	theme	regeneration	549:560	arg1	process					562:568	regeneration process	549:568	regeneration process	549:568	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	5	60	theme	FTIR	750:753	arg1	spectra					755:761	The FTIR spectra	746:761	The FTIR spectra	746:761	The FTIR spectra indicated not only the presence of minute amounts of hemicelluloses and lignin in the filler but also the possible interaction between the matrix and NGCFs.
29408356	8	61	theme	commodity	1313:1321	arg1	polymers					1323:1330	commodity polymers	1313:1330	commodity polymers	1313:1330	The tensile strength of the all-cellulose composites though was lower than that of the cellulose matrix but still was higher than for commodity polymers.
29408356	0	62	theme	grass	63:67	arg1	fillers					86:92	Napier grass cellulose fibril fillers	56:92	Napier grass cellulose fibril fillers	56:92	All-cellulose composite films with cellulose matrix and Napier grass cellulose fibril fillers.
29408356	2	63	theme	cellulose	231:239	arg1	fibrils					241:247	cellulose fibrils	231:247	cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres	231:308	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	2	63	theme	cellulose	231:239	arg1	fillers					323:329	fillers	323:329	fillers	323:329	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	2	63	theme	cellulose	231:239	arg1	CFs					250:252	CFs	250:252	CFs	250:252	In this study, cellulose fibrils (CFs) extracted from native African Napier grass (NG) fibres were used as fillers in cellulose matrix and made all-cellulose composites.
29408356	3	64	theme	cellulose	464:472	arg1	matrix					474:479	cellulose matrix	464:479	cellulose matrix	464:479	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	3	65	from	matrix	474:479	arg1	varied					437:442	varied	437:442	varied	437:442	Napier Grass Cellulose fibrils (NGCFs) loading was varied from 5 to 25 wt% in cellulose matrix in random orientation and the all cellulose composites were made by regeneration process.
29408356	9	66	theme	all-cellulose	1337:1349	arg1	composites					1351:1360	The all-cellulose composites	1333:1360	The all-cellulose composites	1333:1360	The all-cellulose composites can be considered for wrapping and mulching applications.
30553343	0	0	theme	microwave	115:123	arg1	drying					125:130	microwave drying	115:130	microwave drying	115:130	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	4	1	theme	permeability	942:953	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	2	theme	wound	1161:1165	arg1	infection					1167:1175	the seawater immersed wound infection	1139:1175	the seawater immersed wound infection of deep partial-thickness burns	1139:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	4	3	theme	high	847:850	arg1	contents					852:859	high contents	847:859	high contents of activated iodine	847:879	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	4	theme	excellent	991:999	arg1	properties					1015:1024	excellent antibacterial properties	991:1024	excellent antibacterial properties	991:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	5	theme	vapor	936:940	arg1	permeability					942:953	proper water vapor permeability	923:953	proper water vapor permeability	923:953	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	2	6	theme	polymer	386:392	arg1	membranes					394:402	polymer membranes	386:402	polymer membranes	386:402	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	4	7	theme	antibacterial	1001:1013	arg1	properties					1015:1024	excellent antibacterial properties	991:1024	excellent antibacterial properties	991:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	3	8	with	composition	603:613	arg1	alginate					650:657	carboxymethly chitosan/sodium alginate	620:657	carboxymethly chitosan/sodium alginate	620:657	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	4	9	theme	pH	956:957	arg1	release					979:985	pH controllable iodine release	956:985	pH controllable iodine release	956:985	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	10	theme	rat	1126:1128	arg1	model					1130:1134	a rat model	1124:1134	a rat model of the seawater immersed wound infection of deep partial-thickness burns	1124:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	6	11	theme	performance	1288:1298	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	11	theme	performance	1288:1298	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	4	12	theme	hydroxylated	694:705	arg1	lecithin					707:714	hydroxylated lecithin	694:714	hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	694:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	6	13	theme	trauma	1347:1352	arg1	infections					1354:1363	open trauma infections	1342:1363	open trauma infections	1342:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	5	14	theme	partial-thickness	1185:1201	arg1	burns					1203:1207	deep partial-thickness burns	1180:1207	deep partial-thickness burns	1180:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	4	15	theme	HLI	842:844	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	2	16	theme	antibacterial	324:336	arg1	agent					338:342	an important safe inorganic antibacterial agent	296:342	an important safe inorganic antibacterial agent	296:342	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	2	16	theme	antibacterial	324:336	arg1	Iodine					286:291	Iodine	286:291	Iodine	286:291	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	6	17	theme	novel	1215:1219	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	17	theme	novel	1215:1219	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	5	18	theme	burns	1203:1207	arg1	infection					1167:1175	the seawater immersed wound infection	1139:1175	the seawater immersed wound infection of deep partial-thickness burns	1139:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	2	19	theme	inorganic	314:322	arg1	agent					338:342	an important safe inorganic antibacterial agent	296:342	an important safe inorganic antibacterial agent	296:342	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	2	19	theme	inorganic	314:322	arg1	Iodine					286:291	Iodine	286:291	Iodine	286:291	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	1	20	theme	antibacterial	201:213	arg1	properties					215:224	the antibacterial properties	197:224	the antibacterial properties of membrane wound dressings of natural polymers	197:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	4	21	theme	properties	911:920	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	22	theme	composite	1045:1053	arg1	membranes					1055:1063	The composite membranes	1041:1063	The composite membranes	1041:1063	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	2	23	theme	safe	309:312	arg1	agent					338:342	an important safe inorganic antibacterial agent	296:342	an important safe inorganic antibacterial agent	296:342	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	2	23	theme	safe	309:312	arg1	Iodine					286:291	Iodine	286:291	Iodine	286:291	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	3	24	theme	present	477:483	arg1	work					485:488	the present work	473:488	the present work	473:488	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	4	25	theme	microwave	676:684	arg1	drying					686:691	microwave drying	676:691	microwave drying	676:691	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	26	theme	water	930:934	arg1	permeability					942:953	proper water vapor permeability	923:953	proper water vapor permeability	923:953	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	27	dep	membranes	796:804	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	6	28	used	used	1273:1276	arg2	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	28	used	used	1273:1276	arg2	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	4	29	theme	proper	923:928	arg1	permeability					942:953	proper water vapor permeability	923:953	proper water vapor permeability	923:953	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	6	30	theme	open	1342:1345	arg1	infections					1354:1363	open trauma infections	1342:1363	open trauma infections	1342:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	4	31	dep	the	665:667	arg1	aid					669:671	aid	669:671	aid	669:671	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	1	32	theme	membrane	229:236	arg1	dressings					244:252	membrane wound dressings	229:252	membrane wound dressings of natural polymers	229:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	0	33	theme	lecithin	28:35	arg1	Preparation					0:10	Preparation	0:10	Preparation of hydroxylated lecithin	0:35	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	0	34	theme	infected	156:163	arg1	treatment					176:184	infected burn wound treatment	156:184	infected burn wound treatment	156:184	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	3	35	theme	complexing	573:582	arg1	efficiency					584:593	complexing efficiency	573:593	complexing efficiency	573:593	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	4	36	theme	mechanical	887:896	arg1	properties					911:920	good mechanical and swelling properties	882:920	good mechanical and swelling properties	882:920	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	1	37	theme	wound	238:242	arg1	dressings					244:252	membrane wound dressings	229:252	membrane wound dressings of natural polymers	229:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	0	38	theme	hydroxylated	15:26	arg1	lecithin					28:35	hydroxylated lecithin	15:35	hydroxylated lecithin	15:35	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	4	39	theme	contents	852:859	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	40	theme	properties	1015:1024	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	41	theme	alginate	763:770	arg1	membranes					796:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	1	42	theme	dressings	244:252	arg1	properties					215:224	the antibacterial properties	197:224	the antibacterial properties of membrane wound dressings of natural polymers	197:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	0	43	theme	iodine/carboxymethyl	47:66	arg1	membrane					103:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	6	44	theme	composite	1235:1243	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	44	theme	composite	1235:1243	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	0	45	theme	wound	170:174	arg1	treatment					176:184	infected burn wound treatment	156:184	infected burn wound treatment	156:184	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	5	46	theme	infection	1167:1175	arg1	model					1130:1134	a rat model	1124:1134	a rat model of the seawater immersed wound infection of deep partial-thickness burns	1124:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	3	47	theme	carboxymethly	620:632	arg1	alginate					650:657	carboxymethly chitosan/sodium alginate	620:657	carboxymethly chitosan/sodium alginate	620:657	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	6	48	theme	wound	1300:1304	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	48	theme	wound	1300:1304	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	2	49	theme	stability	445:453	arg1	challenges					415:424	the challenges	411:424	the challenges of homogeneity and stability during drying	411:467	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	2	50	theme	homogeneity	429:439	arg1	challenges					415:424	the challenges	411:424	the challenges of homogeneity and stability during drying	411:467	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	0	51	theme	burn	165:168	arg1	treatment					176:184	infected burn wound treatment	156:184	infected burn wound treatment	156:184	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	3	52	theme	chitosan/sodium	634:648	arg1	alginate					650:657	carboxymethly chitosan/sodium alginate	620:657	carboxymethly chitosan/sodium alginate	620:657	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	5	53	theme	deep	1180:1183	arg1	burns					1203:1207	deep partial-thickness burns	1180:1207	deep partial-thickness burns	1180:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	1	54	theme	natural	257:263	arg1	polymers					265:272	natural polymers	257:272	natural polymers	257:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	0	55	theme	alginate	84:91	arg1	membrane					103:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	4	56	theme	iodine	972:977	arg1	release					979:985	pH controllable iodine release	956:985	pH controllable iodine release	956:985	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	2	57	with	composition	369:379	arg1	membranes					394:402	polymer membranes	386:402	polymer membranes	386:402	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	4	58	theme	swelling	902:909	arg1	properties					911:920	good mechanical and swelling properties	882:920	good mechanical and swelling properties	882:920	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	3	59	theme	hydroxylated	517:528	arg1	HL					540:541	HL	540:541	HL	540:541	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	3	59	theme	hydroxylated	517:528	arg1	lecithin					530:537	hydroxylated lecithin	517:537	hydroxylated lecithin (HL)	517:542	In the present work, iodine was complexed with hydroxylated lecithin (HL) to improve its stability and complexing efficiency for the composition with carboxymethly chitosan/sodium alginate.
30553343	0	60	theme	chitosan/sodium	68:82	arg1	membrane					103:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	6	61	theme	high	1283:1286	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	61	theme	high	1283:1286	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	4	62	theme	controllable	959:970	arg1	release					979:985	pH controllable iodine release	956:985	pH controllable iodine release	956:985	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	0	63	from	applications	140:151	arg1	treatment					176:184	infected burn wound treatment	156:184	infected burn wound treatment	156:184	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	5	64	theme	model	1130:1134	arg1	infection					1111:1119	the infection	1107:1119	the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns	1107:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	4	65	theme	HLI/CMCS/SA	773:783	arg1	membranes					796:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	66	theme	immersed	1152:1159	arg1	infection					1167:1175	the seawater immersed wound infection	1139:1175	the seawater immersed wound infection of deep partial-thickness burns	1139:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	4	67	theme	activated	864:872	arg1	iodine					874:879	activated iodine	864:879	activated iodine	864:879	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	2	68	theme	important	299:307	arg1	agent					338:342	an important safe inorganic antibacterial agent	296:342	an important safe inorganic antibacterial agent	296:342	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	2	68	theme	important	299:307	arg1	Iodine					286:291	Iodine	286:291	Iodine	286:291	Iodine is an important safe inorganic antibacterial agent, but was confined in the composition with polymer membranes due to the challenges of homogeneity and stability during drying.
30553343	4	69	theme	good	882:885	arg1	properties					911:920	good mechanical and swelling properties	882:920	good mechanical and swelling properties	882:920	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	6	70	theme	antibacterial	1221:1233	arg1	dressing					1306:1313	a high performance wound dressing	1281:1313	a high performance wound dressing for treating and repairing open trauma infections	1281:1363	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	6	70	theme	antibacterial	1221:1233	arg1	membrane					1245:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane	1210:1252	This novel antibacterial composite membrane can be potentially used as a high performance wound dressing for treating and repairing open trauma infections.
30553343	1	71	theme	polymers	265:272	arg1	dressings					244:252	membrane wound dressings	229:252	membrane wound dressings of natural polymers	229:272	Improving the antibacterial properties of membrane wound dressings of natural polymers is crucial.
30553343	4	72	theme	release	979:985	arg1	distributions					825:837	homogeneously distributions	811:837	homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties	811:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	73	theme	seawater	1143:1150	arg1	infection					1167:1175	the seawater immersed wound infection	1139:1175	the seawater immersed wound infection of deep partial-thickness burns	1139:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	5	74	theme	high	1075:1078	arg1	efficiencies					1090:1101	high repairing efficiencies	1075:1101	high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns	1075:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
30553343	4	75	theme	composite	786:794	arg1	membranes					796:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	0	76	from	drying	125:130	arg1	treatment					176:184	infected burn wound treatment	156:184	infected burn wound treatment	156:184	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	4	77	theme	chitosan/sodium	747:761	arg1	membranes					796:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	chitosan/sodium alginate (HLI/CMCS/SA) composite membranes	747:804	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	0	78	theme	composite	93:101	arg1	membrane					103:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	iodine/carboxymethyl chitosan/sodium alginate composite membrane	47:110	Preparation of hydroxylated lecithin complexed iodine/carboxymethyl chitosan/sodium alginate composite membrane by microwave drying and its applications in infected burn wound treatment.
30553343	4	79	theme	iodine	874:879	arg1	properties					911:920	good mechanical and swelling properties	882:920	good mechanical and swelling properties	882:920	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	79	theme	iodine	874:879	arg1	properties					1015:1024	excellent antibacterial properties	991:1024	excellent antibacterial properties	991:1024	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	79	theme	iodine	874:879	arg1	HLI					842:844	HLI	842:844	HLI	842:844	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	79	theme	iodine	874:879	arg1	contents					852:859	high contents	847:859	high contents of activated iodine	847:879	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	79	theme	iodine	874:879	arg1	release					979:985	pH controllable iodine release	956:985	pH controllable iodine release	956:985	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	4	79	theme	iodine	874:879	arg1	permeability					942:953	proper water vapor permeability	923:953	proper water vapor permeability	923:953	With the aid of microwave drying, hydroxylated lecithin complexed iodine/carboxymethly chitosan/sodium alginate (HLI/CMCS/SA) composite membranes with homogeneously distributions of HLI, high contents of activated iodine, good mechanical and swelling properties, proper water vapor permeability, pH controllable iodine release and excellent antibacterial properties were prepared.
30553343	5	80	theme	repairing	1080:1088	arg1	efficiencies					1090:1101	high repairing efficiencies	1075:1101	high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns	1075:1207	The composite membranes exhibited high repairing efficiencies for the infection of a rat model of the seawater immersed wound infection of deep partial-thickness burns.
31760019	6	0	theme	zinc	852:855	arg1	NPs					863:865	zinc oxide NPs	852:865	zinc oxide NPs	852:865	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	1	theme	tensile	674:680	arg1	22.1-35.5 MPa					692:704	22.1-35.5 MPa	692:704	22.1-35.5 MPa	692:704	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	1	theme	tensile	674:680	arg1	strength					682:689	The tensile strength	670:689	The tensile strength (22.1-35.5 MPa)	670:705	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	1	2	theme	xanthan	149:155	arg1	X					162:162	X	162:162	X	162:162	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	1	2	theme	xanthan	149:155	arg1	gum					157:159	xanthan gum	149:159	xanthan gum (X)	149:163	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	4	3	theme	GXZ	528:530	arg1	nanocomposites					532:545	the GXZ nanocomposites	524:545	the GXZ nanocomposites	524:545	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	7	4	theme	nanocomposites	1047:1060	arg1	permeability					999:1010	the water vapor permeability	983:1010	the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites	983:1060	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	7	4	theme	nanocomposites	1047:1060	arg1	Pas					1035:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	6	5	theme	glass	750:754	arg1	transition					756:765	glass transition	750:765	glass transition temperature (69.9-74.8 °C) of the nanocomposite films	750:819	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	1	6	theme	gum	157:159	arg1	nanocomposites					171:184	Gellan (G) and xanthan gum (X) based nanocomposites	134:184	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs)	134:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	7	7	from	decrease	1076:1083	arg1	hydrophilicity					1088:1101	hydrophilicity	1088:1101	hydrophilicity	1088:1101	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	8	8	theme	light	1162:1166	arg1	shielding					1168:1176	higher ultra-violet light shielding	1142:1176	higher ultra-violet light shielding	1142:1176	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	7	9	theme	zinc	913:916	arg1	oxide					918:922	zinc oxide	913:922	zinc oxide NPs	913:926	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	9	10	theme	pharmaceutical	1350:1363	arg1	applications					1375:1386	food and pharmaceutical packaging applications	1341:1386	food and pharmaceutical packaging applications	1341:1386	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	6	11	theme	thermal	708:714	arg1	-82.5-96.2 °C					730:742	T5%-82.5-96.2 °C	727:742	T5%-82.5-96.2 °C	727:742	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	11	theme	thermal	708:714	arg1	stability					716:724	thermal stability	708:724	thermal stability (T5%-82.5-96.2 °C)	708:743	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	4	12	theme	nanocomposites	532:545	arg1	modulus					513:519	The storage and loss modulus	492:519	modulus	513:519	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	7	13	theme	3.83-2.31 × 10-9 g/m2	1013:1033	arg1	permeability					999:1010	the water vapor permeability	983:1010	the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites	983:1060	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	7	13	theme	3.83-2.31 × 10-9 g/m2	1013:1033	arg1	Pas					1035:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	9	14	theme	packaging	1365:1373	arg1	applications					1375:1386	food and pharmaceutical packaging applications	1341:1386	food and pharmaceutical packaging applications	1341:1386	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	3	15	theme	prepared	371:378	arg1	nanocomposites					380:393	The prepared nanocomposites	367:393	The prepared nanocomposites	367:393	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	2	16	theme	films	347:351	arg1	morphology					292:301	morphology	292:301	morphology	292:301	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	2	16	theme	films	347:351	arg1	structure					281:289	structure	281:289	structure	281:289	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	2	16	theme	films	347:351	arg1	properties					320:329	rheological properties	308:329	rheological properties	308:329	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	0	17	theme	antimicrobial	108:120	arg1	properties					122:131	antimicrobial properties	108:131	antimicrobial properties	108:131	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	8	18	theme	ultra-violet	1149:1160	arg1	shielding					1168:1176	higher ultra-violet light shielding	1142:1176	higher ultra-violet light shielding	1142:1176	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	3	19	from	decrease	435:442	arg1	viscosity					447:455	viscosity	447:455	viscosity	447:455	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	2	20	theme	composite	337:345	arg1	films					347:351	GX composite films	334:351	GX composite films	334:351	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	8	21	theme	nanocomposite	1112:1124	arg1	films					1126:1130	The GXZ nanocomposite films	1104:1130	The GXZ nanocomposite films	1104:1130	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	6	22	theme	films	815:819	arg1	-82.5-96.2 °C					730:742	T5%-82.5-96.2 °C	727:742	T5%-82.5-96.2 °C	727:742	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	22	theme	films	815:819	arg1	temperature					767:777	glass transition temperature	750:777	glass transition temperature (69.9-74.8 °C) of the nanocomposite films	750:819	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	22	theme	films	815:819	arg1	69.9-74.8 °C					780:791	69.9-74.8 °C	780:791	69.9-74.8 °C	780:791	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	22	theme	films	815:819	arg1	stability					716:724	thermal stability	708:724	thermal stability (T5%-82.5-96.2 °C)	708:743	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	22	theme	films	815:819	arg1	strength					682:689	The tensile strength	670:689	The tensile strength (22.1-35.5 MPa)	670:705	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	22	theme	films	815:819	arg1	22.1-35.5 MPa					692:704	22.1-35.5 MPa	692:704	22.1-35.5 MPa	692:704	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	23	theme	%	729:729	arg1	-82.5-96.2 °C					730:742	T5%-82.5-96.2 °C	727:742	T5%-82.5-96.2 °C	727:742	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	23	theme	%	729:729	arg1	stability					716:724	thermal stability	708:724	thermal stability (T5%-82.5-96.2 °C)	708:743	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	2	24	dep	structure	281:289	arg1	the					277:279	the	277:279	the	277:279	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	2	25	theme	GX	334:335	arg1	films					347:351	GX composite films	334:351	GX composite films	334:351	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	8	26	theme	GXZ	1108:1110	arg1	films					1126:1130	The GXZ nanocomposite films	1104:1130	The GXZ nanocomposite films	1104:1130	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	4	27	theme	loss	508:511	arg1	modulus					513:519	The storage and loss modulus	492:519	modulus	513:519	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	6	28	theme	T5	727:728	arg1	-82.5-96.2 °C					730:742	T5%-82.5-96.2 °C	727:742	T5%-82.5-96.2 °C	727:742	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	28	theme	T5	727:728	arg1	stability					716:724	thermal stability	708:724	thermal stability (T5%-82.5-96.2 °C)	708:743	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	2	29	theme	oxide	268:272	arg1	effect					253:258	The effect	249:258	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films	249:351	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	7	30	theme	water	987:991	arg1	permeability					999:1010	the water vapor permeability	983:1010	the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites	983:1060	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	7	30	theme	water	987:991	arg1	Pas					1035:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	1	31	theme	based	165:169	arg1	nanocomposites					171:184	Gellan (G) and xanthan gum (X) based nanocomposites	134:184	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs)	134:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	0	32	theme	gum/xanthan	17:27	arg1	nanocomposites					50:63	gellan gum/xanthan gum/zinc oxide based nanocomposites	10:63	gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application	10:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	5	33	theme	high	654:657	arg1	frequency					659:667	high frequency	654:667	high frequency	654:667	There was a decrease in complex viscosity at high frequency.
31760019	2	34	theme	zinc	263:266	arg1	oxide					268:272	zinc oxide	263:272	zinc oxide	263:272	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	8	35	theme	water	1204:1208	arg1	properties					1218:1227	superior thermal and water barrier properties	1183:1227	properties	1218:1227	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	0	36	theme	gellan	10:15	arg1	nanocomposites					50:63	gellan gum/xanthan gum/zinc oxide based nanocomposites	10:63	gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application	10:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	7	37	theme	vapor	993:997	arg1	permeability					999:1010	the water vapor permeability	983:1010	the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites	983:1060	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	7	37	theme	vapor	993:997	arg1	Pas					1035:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	3.83-2.31 × 10-9 g/m2 Pas	1013:1037	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	6	38	theme	nanocomposite	801:813	arg1	films					815:819	the nanocomposite films	797:819	the nanocomposite films	797:819	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	3	39	theme	shear	466:470	arg1	rate					472:475	the shear rate	462:475	the shear rate	462:475	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	0	40	theme	oxide	38:42	arg1	nanocomposites					50:63	gellan gum/xanthan gum/zinc oxide based nanocomposites	10:63	gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application	10:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	9	41	theme	GXZ	1281:1283	arg1	films					1295:1299	GXZ composite films	1281:1299	GXZ composite films	1281:1299	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	3	42	theme	thinning	411:418	arg1	behavior					420:427	shear thinning behavior	405:427	shear thinning behavior	405:427	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	1	43	theme	zinc	197:200	arg1	Z					209:209	Z	209:209	Z	209:209	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	1	43	theme	zinc	197:200	arg1	oxide					202:206	zinc oxide	197:206	zinc oxide (Z) nanoparticles (NPs)	197:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	0	44	theme	gum/zinc	29:36	arg1	nanocomposites					50:63	gellan gum/xanthan gum/zinc oxide based nanocomposites	10:63	gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application	10:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	9	45	theme	composite	1285:1293	arg1	films					1295:1299	GXZ composite films	1281:1299	GXZ composite films	1281:1299	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	8	46	theme	higher	1142:1147	arg1	shielding					1168:1176	higher ultra-violet light shielding	1142:1176	higher ultra-violet light shielding	1142:1176	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	7	47	theme	contact	942:948	arg1	54.1-60.8o					957:966	54.1-60.8o	957:966	54.1-60.8o	957:966	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	7	47	theme	contact	942:948	arg1	angle					950:954	the contact angle	938:954	the contact angle (54.1-60.8o)	938:967	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	1	48	theme	oxide	202:206	arg1	NPs					227:229	NPs	227:229	NPs	227:229	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	1	48	theme	oxide	202:206	arg1	nanoparticles					212:224	zinc oxide (Z) nanoparticles	197:224	zinc oxide (Z) nanoparticles (NPs)	197:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	0	49	theme	nanocomposites	50:63	arg1	Blends					0:5	Blends	0:5	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.	0:132	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	5	50	from	decrease	621:628	arg1	viscosity					641:649	complex viscosity	633:649	complex viscosity	633:649	There was a decrease in complex viscosity at high frequency.
31760019	3	51	from	behavior	420:427	arg1	viscosity					447:455	viscosity	447:455	viscosity	447:455	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	0	52	theme	based	44:48	arg1	nanocomposites					50:63	gellan gum/xanthan gum/zinc oxide based nanocomposites	10:63	gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application	10:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	4	53	theme	storage	496:502	arg1	modulus					513:519	The storage and loss modulus	492:519	modulus	513:519	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	5	54	theme	complex	633:639	arg1	viscosity					641:649	complex viscosity	633:649	complex viscosity	633:649	There was a decrease in complex viscosity at high frequency.
31760019	1	55	theme	Gellan	134:139	arg1	nanocomposites					171:184	Gellan (G) and xanthan gum (X) based nanocomposites	134:184	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs)	134:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	6	56	theme	NPs	863:865	arg1	addition					840:847	the addition	836:847	the addition of zinc oxide NPs	836:865	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	9	57	theme	food	1341:1344	arg1	applications					1375:1386	food and pharmaceutical packaging applications	1341:1386	food and pharmaceutical packaging applications	1341:1386	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	8	58	theme	composite	1241:1249	arg1	film					1251:1254	the GX composite film	1234:1254	the GX composite film	1234:1254	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	4	59	theme	increased	562:570	arg1	percentage					583:592	increased zinc oxide percentage	562:592	increased zinc oxide percentage	562:592	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	1	60	contain	containing	186:195	arg2	NPs					227:229	NPs	227:229	NPs	227:229	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	1	60	contain	containing	186:195	arg2	nanoparticles					212:224	zinc oxide (Z) nanoparticles	197:224	zinc oxide (Z) nanoparticles (NPs)	197:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	1	60	contain	containing	186:195	arg1	nanocomposites					171:184	Gellan (G) and xanthan gum (X) based nanocomposites	134:184	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs)	134:230	Gellan (G) and xanthan gum (X) based nanocomposites containing zinc oxide (Z) nanoparticles (NPs) were fabricated.
31760019	7	61	theme	oxide	918:922	arg1	NPs					924:926	zinc oxide NPs	913:926	zinc oxide NPs	913:926	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	8	62	theme	GX	1238:1239	arg1	film					1251:1254	the GX composite film	1234:1254	the GX composite film	1234:1254	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	2	63	from	effect	253:258	arg1	morphology					292:301	morphology	292:301	morphology	292:301	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	2	63	from	effect	253:258	arg1	structure					281:289	structure	281:289	structure	281:289	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	2	63	from	effect	253:258	arg1	properties					320:329	rheological properties	308:329	rheological properties	308:329	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	9	64	theme	beneficial	1314:1323	arg1	contribution					1325:1336	a beneficial contribution	1312:1336	a beneficial contribution to food and pharmaceutical packaging applications	1312:1386	Based on these results, GXZ composite films could make a beneficial contribution to food and pharmaceutical packaging applications.
31760019	0	65	theme	packaging	69:77	arg1	application					79:89	packaging application	69:89	packaging application	69:89	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	6	66	theme	transition	756:765	arg1	69.9-74.8 °C					780:791	69.9-74.8 °C	780:791	69.9-74.8 °C	780:791	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	6	66	theme	transition	756:765	arg1	temperature					767:777	glass transition temperature	750:777	glass transition temperature (69.9-74.8 °C) of the nanocomposite films	750:819	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	8	67	theme	barrier	1210:1216	arg1	properties					1218:1227	superior thermal and water barrier properties	1183:1227	properties	1218:1227	The GXZ nanocomposite films exhibited higher ultra-violet light shielding, and superior thermal and water barrier properties than the GX composite film.
31760019	7	68	theme	NPs	924:926	arg1	incorporation					896:908	the incorporation	892:908	the incorporation of zinc oxide NPs	892:926	The results showed that the incorporation of zinc oxide NPs increased the contact angle (54.1-60.8o) and decreased the water vapor permeability (3.83-2.31 × 10-9 g/m2 Pas) of the nanocomposites, indicating a decrease in hydrophilicity.
31760019	3	69	theme	shear	405:409	arg1	behavior					420:427	shear thinning behavior	405:427	shear thinning behavior	405:427	The prepared nanocomposites exhibited shear thinning behavior and a decrease in viscosity when the shear rate was increased.
31760019	4	70	theme	oxide	577:581	arg1	percentage					583:592	increased zinc oxide percentage	562:592	increased zinc oxide percentage	562:592	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31760019	6	71	theme	oxide	857:861	arg1	NPs					863:865	zinc oxide NPs	852:865	zinc oxide NPs	852:865	The tensile strength (22.1-35.5 MPa), thermal stability (T5%-82.5-96.2 °C), and glass transition temperature (69.9-74.8 °C) of the nanocomposite films increased with the addition of zinc oxide NPs.
31760019	0	72	dep	Blends	0:5	arg1	Rheological					92:102	Rheological	92:102	Rheological	92:102	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	0	72	dep	Blends	0:5	arg1	properties					122:131	antimicrobial properties	108:131	antimicrobial properties	108:131	Blends of gellan gum/xanthan gum/zinc oxide based nanocomposites for packaging application: Rheological and antimicrobial properties.
31760019	2	73	theme	rheological	308:318	arg1	properties					320:329	rheological properties	308:329	rheological properties	308:329	The effect of zinc oxide on the structure, morphology, and rheological properties of GX composite films were studied.
31760019	4	74	theme	zinc	572:575	arg1	percentage					583:592	increased zinc oxide percentage	562:592	increased zinc oxide percentage	562:592	The storage and loss modulus of the GXZ nanocomposites increased with increased zinc oxide percentage and frequency.
31755373	4	0	theme	above	585:589	arg1	composites					591:600	the above composites	581:600	the above composites	581:600	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31755373	7	1	theme	optimal	1027:1033	arg1	range					1042:1046	the optimal dosage range	1023:1046	the optimal dosage range	1023:1046	% as the optimal dosage range.
31755373	2	2	theme	rattan	454:459	arg1	advantages					403:412	advantages	403:412	advantages of both natural rattan and pure plastic rattan	403:459	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	3	3	theme	main	466:469	arg1	objective					471:479	The main objective	462:479	The main objective of the work	462:491	The main objective of the work was to improve the properties of SBR by corn starch (CS).
31755373	6	4	theme	composite	951:959	arg1	properties					918:927	the mechanical properties	903:927	the mechanical properties	903:927	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	6	4	theme	composite	951:959	arg1	index					938:942	melt index	933:942	melt index	933:942	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	6	4	theme	composite	951:959	arg1	composite					951:959	the composite	947:959	the composite	947:959	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	6	4	theme	composite	951:959	arg1	%					900:900	%	900:900	%	900:900	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	2	5	theme	plastic	446:452	arg1	rattan					454:459	pure plastic rattan	441:459	pure plastic rattan	441:459	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	6	6	theme	mechanical	907:916	arg1	properties					918:927	the mechanical properties	903:927	the mechanical properties	903:927	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	6	7	theme	melt	933:936	arg1	index					938:942	melt index	933:942	melt index	933:942	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	2	8	theme	pure	441:444	arg1	rattan					454:459	pure plastic rattan	441:459	pure plastic rattan	441:459	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	9	theme	biomass	300:306	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	9	theme	biomass	300:306	arg1	SBR					316:318	SBR	316:318	SBR	316:318	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	9	theme	biomass	300:306	arg1	rattan					308:313	straw biomass rattan	294:313	straw biomass rattan (SBR) as weaving material	294:339	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	4	10	theme	SBR	622:624	arg1	experiments					607:617	the experiments	603:617	the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures	603:757	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31755373	4	11	theme	mechanical	651:660	arg1	properties					662:671	mechanical properties	651:671	mechanical properties	651:671	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31755373	9	12	theme	SBR	1193:1195	arg1	microstructure					1175:1188	the microstructure	1171:1188	the microstructure of SBR	1171:1195	With CS added, the microstructure of SBR was examined by scanning electron microscope and found the microscopic surfaces and sections to become smoother, and that could improve the compatibility and tenacity between the materials.
31755373	9	13	theme	microscopic	1256:1266	arg1	surfaces					1268:1275	the microscopic surfaces	1252:1275	the microscopic surfaces	1252:1275	With CS added, the microstructure of SBR was examined by scanning electron microscope and found the microscopic surfaces and sections to become smoother, and that could improve the compatibility and tenacity between the materials.
31755373	2	14	theme	developmental	272:284	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	14	theme	developmental	272:284	arg1	work					381:384	per our previous work	364:384	per our previous work	364:384	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	14	theme	developmental	272:284	arg1	rattan					308:313	straw biomass rattan	294:313	straw biomass rattan (SBR) as weaving material	294:339	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	15	theme	weaving	324:330	arg1	material					332:339	weaving material	324:339	weaving material	324:339	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	16	theme	previous	372:379	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	16	theme	previous	372:379	arg1	work					381:384	per our previous work	364:384	per our previous work	364:384	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	10	17	theme	future	1562:1567	arg1	perspectives					1569:1580	future perspectives	1562:1580	future perspectives	1562:1580	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	2	18	contain	possessed	393:401	arg2	advantages					403:412	advantages	403:412	advantages of both natural rattan and pure plastic rattan	403:459	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	18	contain	possessed	393:401	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	18	contain	possessed	393:401	arg1	work					381:384	per our previous work	364:384	per our previous work	364:384	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	10	19	theme	mechanical	1518:1527	arg1	properties					1529:1538	the mechanical properties	1514:1538	the mechanical properties of the composites for future perspectives	1514:1580	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	6	20	dep	increased	966:974	arg1	decreased					991:999	decreased	991:999	decreased	991:999	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	1	21	theme	promising	227:235	arg1	materials					251:259	the most promising biodegradable materials	218:259	the most promising biodegradable materials	218:259	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	5	22	theme	CS	832:833	arg1	content					821:827	the content	817:827	the content of CS	817:833	The results revealed that when the content of CS increased gradually within the range of 0, 3, 6, 9 12, and 15 wt.
31755373	9	23	theme	electron	1222:1229	arg1	microscope					1231:1240	scanning electron microscope	1213:1240	scanning electron microscope	1213:1240	With CS added, the microstructure of SBR was examined by scanning electron microscope and found the microscopic surfaces and sections to become smoother, and that could improve the compatibility and tenacity between the materials.
31755373	2	24	theme	per	364:366	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	24	theme	per	364:366	arg1	work					381:384	per our previous work	364:384	per our previous work	364:384	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	1	25	theme	biodegradable	237:249	arg1	materials					251:259	the most promising biodegradable materials	218:259	the most promising biodegradable materials	218:259	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	10	26	used	used	1424:1427	arg2	result					1392:1397	a result	1390:1397	a result	1390:1397	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	10	26	used	used	1424:1427	arg2	supplement					1434:1443	a supplement	1432:1443	a supplement	1432:1443	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	10	26	used	used	1424:1427	arg2	CS					1400:1401	CS	1400:1401	CS in moderation	1400:1415	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	5	27	theme	wt	897:898	arg1	range					866:870	the range	862:870	the range of 0, 3, 6, 9 12, and 15 wt	862:898	The results revealed that when the content of CS increased gradually within the range of 0, 3, 6, 9 12, and 15 wt.
31755373	1	28	theme	renewable	78:86	arg1	resource					88:95	renewable resource	78:95	renewable resource	78:95	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	9	29	dep	compatibility	1337:1349	arg1	the					1333:1335	the	1333:1335	the	1333:1335	With CS added, the microstructure of SBR was examined by scanning electron microscope and found the microscopic surfaces and sections to become smoother, and that could improve the compatibility and tenacity between the materials.
31755373	0	30	theme	biomass	34:40	arg1	rattan					42:47	straw biomass rattan	28:47	straw biomass rattan	28:47	Improving the properties of straw biomass rattan by corn starch.
31755373	1	31	theme	materials	251:259	arg1	one					211:213	one	211:213	one	211:213	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	31	theme	materials	251:259	arg1	materials					251:259	the most promising biodegradable materials	218:259	the most promising biodegradable materials	218:259	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	31	theme	materials	251:259	arg1	starch					118:123	starch	118:123	starch	118:123	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	7	32	dep	%	1018:1018	arg1	range					1042:1046	the optimal dosage range	1023:1046	the optimal dosage range	1023:1046	% as the optimal dosage range.
31755373	4	33	theme	melting	674:680	arg1	performance					682:692	melting performance	674:692	melting performance	674:692	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31755373	9	34	theme	scanning	1213:1220	arg1	microscope					1231:1240	scanning electron microscope	1213:1240	scanning electron microscope	1213:1240	With CS added, the microstructure of SBR was examined by scanning electron microscope and found the microscopic surfaces and sections to become smoother, and that could improve the compatibility and tenacity between the materials.
31755373	0	35	theme	straw	28:32	arg1	rattan					42:47	straw biomass rattan	28:47	straw biomass rattan	28:47	Improving the properties of straw biomass rattan by corn starch.
31755373	10	36	theme	composites	1547:1556	arg1	properties					1529:1538	the mechanical properties	1514:1538	the mechanical properties of the composites for future perspectives	1514:1580	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	10	37	from	CS	1400:1401	arg1	moderation					1406:1415	moderation	1406:1415	moderation	1406:1415	As a result, CS in moderation can be used as a supplement to enhance SBR, and improve their characteristics which will enhance the mechanical properties of the composites for future perspectives.
31755373	1	38	theme	modern	176:181	arg1	industry					183:190	the modern industry	172:190	the modern industry	172:190	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	8	39	theme	water	1066:1070	arg1	absorption					1072:1081	the water absorption	1062:1081	the water absorption of SBR	1062:1088	In contrast, the water absorption of SBR kept increased in this range, indicating an easier biodegradable.
31755373	8	40	theme	easier	1134:1139	arg1	biodegradable					1141:1153	an easier biodegradable	1131:1153	an easier biodegradable	1131:1153	In contrast, the water absorption of SBR kept increased in this range, indicating an easier biodegradable.
31755373	2	41	theme	natural	422:428	arg1	rattan					430:435	natural rattan	422:435	natural rattan	422:435	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	4	42	theme	thermogravimetric	711:727	arg1	analysis					729:736	thermogravimetric analysis	711:736	thermogravimetric analysis	711:736	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31755373	1	43	theme	resource	88:95	arg1	kind					70:73	a kind	68:73	a kind of renewable resource and natural biomass	68:115	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	43	theme	resource	88:95	arg1	starch					118:123	starch	118:123	starch	118:123	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	0	44	theme	rattan	42:47	arg1	properties					14:23	the properties	10:23	the properties of straw biomass rattan	10:47	Improving the properties of straw biomass rattan by corn starch.
31755373	2	45	theme	straw	294:298	arg1	rattan					286:291	The newly developmental rattan	262:291	The newly developmental rattan	262:291	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	45	theme	straw	294:298	arg1	SBR					316:318	SBR	316:318	SBR	316:318	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	2	45	theme	straw	294:298	arg1	rattan					308:313	straw biomass rattan	294:313	straw biomass rattan (SBR) as weaving material	294:339	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	3	46	theme	work	488:491	arg1	objective					471:479	The main objective	462:479	The main objective of the work	462:491	The main objective of the work was to improve the properties of SBR by corn starch (CS).
31755373	1	47	theme	natural	101:107	arg1	biomass					109:115	natural biomass	101:115	natural biomass	101:115	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	3	48	theme	corn	533:536	arg1	CS					546:547	CS	546:547	CS	546:547	The main objective of the work was to improve the properties of SBR by corn starch (CS).
31755373	3	48	theme	corn	533:536	arg1	starch					538:543	corn starch	533:543	corn starch (CS)	533:548	The main objective of the work was to improve the properties of SBR by corn starch (CS).
31755373	1	49	theme	biomass	109:115	arg1	kind					70:73	a kind	68:73	a kind of renewable resource and natural biomass	68:115	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	49	theme	biomass	109:115	arg1	starch					118:123	starch	118:123	starch	118:123	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	6	50	dep	12	1012:1013	arg1	to					1009:1010	to	1009:1010	to	1009:1010	%, the mechanical properties and melt index of the composite both increased first and then decreased, with 6 to 12 wt.
31755373	2	51	theme	rattan	430:435	arg1	advantages					403:412	advantages	403:412	advantages of both natural rattan and pure plastic rattan	403:459	The newly developmental rattan, straw biomass rattan (SBR) as weaving material, has been exploited as per our previous work, which possessed advantages of both natural rattan and pure plastic rattan.
31755373	1	52	used	used	141:144	arg2	starch					118:123	starch	118:123	starch	118:123	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	52	used	used	141:144	arg2	materials					251:259	the most promising biodegradable materials	218:259	the most promising biodegradable materials	218:259	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	52	used	used	141:144	arg2	kind					70:73	a kind	68:73	a kind of renewable resource and natural biomass	68:115	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	1	52	used	used	141:144	arg2	one					211:213	one	211:213	one	211:213	As a kind of renewable resource and natural biomass, starch has been widely used to substitute plastics in the modern industry and is regarded as one of the most promising biodegradable materials.
31755373	7	53	theme	dosage	1035:1040	arg1	range					1042:1046	the optimal dosage range	1023:1046	the optimal dosage range	1023:1046	% as the optimal dosage range.
31755373	0	54	theme	corn	52:55	arg1	starch					57:62	corn starch	52:62	corn starch	52:62	Improving the properties of straw biomass rattan by corn starch.
31755373	3	55	theme	SBR	526:528	arg1	properties					512:521	the properties	508:521	the properties of SBR	508:528	The main objective of the work was to improve the properties of SBR by corn starch (CS).
31755373	8	56	theme	SBR	1086:1088	arg1	absorption					1072:1081	the water absorption	1062:1081	the water absorption of SBR	1062:1088	In contrast, the water absorption of SBR kept increased in this range, indicating an easier biodegradable.
31755373	4	57	theme	composites	591:600	arg1	manufacturing					564:576	the manufacturing	560:576	the manufacturing of the above composites	560:600	Based on the manufacturing of the above composites, the experiments of SBR that enhanced with CS on mechanical properties, melting performance, hydroscopicity, thermogravimetric analysis, and microstructures were tested in this study.
31284990	5	0	theme	High	711:714	arg1	sequencing					727:736	High throughput sequencing	711:736	High throughput sequencing	711:736	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	5	1	from	enrichment	750:759	arg1	diversity					768:776	the diversity	764:776	the diversity	764:776	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	5	1	from	enrichment	750:759	arg1	composition					803:813	the composition	799:813	the composition of the fecal microbiota	799:837	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	1	2	theme	auricular	157:165	arg1	AAP					184:186	AAP	184:186	AAP	184:186	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	2	theme	auricular	157:165	arg1	polysaccharides					167:181	A. auricular polysaccharides	154:181	A. auricular polysaccharides (AAP)	154:187	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	3	theme	microbe	251:257	arg1	diversity					223:231	diversity	223:231	diversity	223:231	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	3	theme	microbe	251:257	arg1	composition					207:217	composition	207:217	composition	207:217	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	5	4	theme	throughput	716:725	arg1	sequencing					727:736	High throughput sequencing	711:736	High throughput sequencing	711:736	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	1	5	theme	polysaccharides	167:181	arg1	contribution					138:149	the contribution	134:149	the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice	134:265	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	0	6	from	Consumption	0:10	arg1	auricular					48:56	Auricularia auricular	36:56	Auricularia auricular	36:56	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	6	7	from	increase	1072:1079	arg1	P < .05					1118:1124	P < .05	1118:1124	P < .05	1118:1124	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	6	7	from	increase	1072:1079	arg1	concentrations					1102:1115	serum IgA and IgG concentrations	1084:1115	serum IgA and IgG concentrations (P < .05)	1084:1125	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	3	8	theme	intestinal	501:510	arg1	environment					512:522	the intestinal environment	497:522	the intestinal environment in the mice	497:534	AAP feeding changed the intestinal environment in the mice.
31284990	2	9	theme	A.	291:292	arg1	powder					316:321	A. auricula freeze-dried powder	291:321	A. auricula freeze-dried powder	291:321	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	7	10	theme	mushroom	1315:1322	arg1	consumption					1296:1306	the consumption	1292:1306	the consumption of the mushroom	1292:1322	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	4	11	theme	dependent	657:665	arg1	manner					667:672	a dose dependent manner	650:672	a dose dependent manner	650:672	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	6	12	theme	serum	1084:1088	arg1	IgA					1090:1092	serum IgA	1084:1092	serum IgA	1084:1092	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	2	13	theme	AAP/kg	368:373	arg1	weight					380:385	40, 80, 160 mg AAP/kg body weight	353:385	40, 80, 160 mg AAP/kg body weight	353:385	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	13	theme	AAP/kg	368:373	arg1	AAP					348:350	AAP	348:350	AAP (40, 80, 160 mg AAP/kg body weight)	348:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	0	14	from	auricular	48:56	arg1	polysaccharides					15:29	polysaccharides	15:29	polysaccharides from Auricularia auricular	15:56	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	0	14	from	auricular	48:56	arg1	Consumption					0:10	Consumption	0:10	Consumption of polysaccharides from Auricularia auricular	0:56	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	4	15	theme	pH	541:542	arg1	value					544:548	The pH value	537:548	The pH value of the intestinal compartments	537:579	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	7	16	theme	beneficial	1268:1277	arg1	effects					1279:1285	the reported beneficial effects	1255:1285	the reported beneficial effects from the consumption of the mushroom	1255:1322	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	6	17	theme	fed	1027:1029	arg1	mice					1031:1034	the AAP fed mice	1019:1034	the AAP fed mice	1019:1034	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	2	18	theme	male	420:423	arg1	mice					429:432	6 week-old male ICR mice	409:432	6 week-old male ICR mice	409:432	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	5	19	theme	significant	866:876	arg1	decrease					878:885	a significant decrease	864:885	a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05)	864:933	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	6	20	theme	AAP	1023:1025	arg1	mice					1031:1034	the AAP fed mice	1019:1034	the AAP fed mice	1019:1034	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	2	21	theme	6 week-old	409:418	arg1	mice					429:432	6 week-old male ICR mice	409:432	6 week-old male ICR mice	409:432	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	4	22	theme	AAP-fed	631:637	arg1	groups					639:644	AAP-fed groups	631:644	AAP-fed groups	631:644	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	6	23	theme	IgG	1098:1100	arg1	P < .05					1118:1124	P < .05	1118:1124	P < .05	1118:1124	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	6	23	theme	IgG	1098:1100	arg1	concentrations					1102:1115	serum IgA and IgG concentrations	1084:1115	serum IgA and IgG concentrations (P < .05)	1084:1125	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	6	24	theme	relative	940:947	arg1	abundances					949:958	The relative abundances	936:958	The relative abundances of Porphyromonadaceae and Bacteroidaceae	936:999	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	5	25	theme	AAP	846:848	arg1	mice					854:857	the AAP fed mice	842:857	the AAP fed mice	842:857	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	1	26	from	mice	262:265	arg1	diversity					223:231	diversity	223:231	diversity	223:231	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	26	from	mice	262:265	arg1	composition					207:217	composition	207:217	composition	207:217	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	4	27	theme	SCFAs	597:601	arg1	concentrations					603:616	SCFAs concentrations	597:616	SCFAs concentrations	597:616	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	0	28	theme	polysaccharides	15:29	arg1	Consumption					0:10	Consumption	0:10	Consumption of polysaccharides from Auricularia auricular	0:56	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	2	29	theme	AAP	348:350	arg1	amounts					337:343	different amounts	327:343	different amounts of AAP (40, 80, 160 mg AAP/kg body weight)	327:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	29	theme	AAP	348:350	arg1	weight					380:385	40, 80, 160 mg AAP/kg body weight	353:385	40, 80, 160 mg AAP/kg body weight	353:385	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	29	theme	AAP	348:350	arg1	AAP					348:350	AAP	348:350	AAP (40, 80, 160 mg AAP/kg body weight)	348:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	5	30	theme	microbiota	828:837	arg1	composition					803:813	the composition	799:813	the composition of the fecal microbiota	799:837	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	1	31	from	microbe	251:257	arg1	mice					262:265	mice	262:265	mice	262:265	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	7	32	theme	mouse	1185:1189	arg1	microbiota					1195:1204	the mouse gut microbiota	1181:1204	the mouse gut microbiota	1181:1204	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	5	33	theme	fecal	822:826	arg1	microbiota					828:837	the fecal microbiota	818:837	the fecal microbiota	818:837	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	0	34	theme	Auricularia	36:46	arg1	auricular					48:56	Auricularia auricular	36:56	Auricularia auricular	36:56	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	0	35	from	microbiota	83:92	arg1	mice					97:100	mice	97:100	mice	97:100	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	1	36	from	composition	207:217	arg1	mice					262:265	mice	262:265	mice	262:265	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	2	37	theme	body	375:378	arg1	weight					380:385	40, 80, 160 mg AAP/kg body weight	353:385	40, 80, 160 mg AAP/kg body weight	353:385	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	37	theme	body	375:378	arg1	AAP					348:350	AAP	348:350	AAP (40, 80, 160 mg AAP/kg body weight)	348:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	6	38	theme	Bacteroidaceae	986:999	arg1	abundances					949:958	The relative abundances	936:958	The relative abundances of Porphyromonadaceae and Bacteroidaceae	936:999	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	5	39	theme	fed	850:852	arg1	mice					854:857	the AAP fed mice	842:857	the AAP fed mice	842:857	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	2	40	theme	ICR	425:427	arg1	mice					429:432	6 week-old male ICR mice	409:432	6 week-old male ICR mice	409:432	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	7	41	from	study	1151:1155	arg1	findings					1132:1139	The findings	1128:1139	The findings from this study	1128:1155	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	2	42	dep	A.	291:292	arg1	auricula					294:301	auricula	294:301	auricula	294:301	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	42	dep	A.	291:292	arg1	freeze-dried					303:314	freeze-dried	303:314	freeze-dried	303:314	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	1	43	from	diversity	223:231	arg1	mice					262:265	mice	262:265	mice	262:265	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	7	44	theme	reported	1259:1266	arg1	effects					1279:1285	the reported beneficial effects	1255:1285	the reported beneficial effects from the consumption of the mushroom	1255:1322	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	1	45	dep	composition	207:217	arg1	the					203:205	the	203:205	the	203:205	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	5	46	from	alteration	785:794	arg1	diversity					768:776	the diversity	764:776	the diversity	764:776	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	5	46	from	alteration	785:794	arg1	composition					803:813	the composition	799:813	the composition of the fecal microbiota	799:837	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	2	47	theme	different	327:335	arg1	amounts					337:343	different amounts	327:343	different amounts of AAP (40, 80, 160 mg AAP/kg body weight)	327:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	47	theme	different	327:335	arg1	weight					380:385	40, 80, 160 mg AAP/kg body weight	353:385	40, 80, 160 mg AAP/kg body weight	353:385	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	2	47	theme	different	327:335	arg1	AAP					348:350	AAP	348:350	AAP (40, 80, 160 mg AAP/kg body weight)	348:386	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	7	48	from	consumption	1296:1306	arg1	effects					1279:1285	the reported beneficial effects	1255:1285	the reported beneficial effects from the consumption of the mushroom	1255:1322	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	4	49	theme	intestinal	557:566	arg1	compartments					568:579	the intestinal compartments	553:579	the intestinal compartments	553:579	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	5	50	theme	Firmicutes/Bacteroidetes	894:917	arg1	ratio					919:923	the Firmicutes/Bacteroidetes ratio	890:923	the Firmicutes/Bacteroidetes ratio (P < .05)	890:933	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	5	50	theme	Firmicutes/Bacteroidetes	894:917	arg1	P < .05					926:932	P < .05	926:932	P < .05	926:932	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	7	51	theme	gut	1191:1193	arg1	microbiota					1195:1204	the mouse gut microbiota	1181:1204	the mouse gut microbiota	1181:1204	The findings from this study show that AAP modulates the mouse gut microbiota and may contribute, at least in part, to some of the reported beneficial effects from the consumption of the mushroom, A. auricula.
31284990	6	52	theme	Porphyromonadaceae	963:980	arg1	abundances					949:958	The relative abundances	936:958	The relative abundances of Porphyromonadaceae and Bacteroidaceae	936:999	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	2	53	theme	five-week	459:467	arg1	period					469:474	a five-week period	457:474	a five-week period	457:474	AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding over a five-week period.
31284990	3	54	from	environment	512:522	arg1	mice					531:534	the mice	527:534	the mice	527:534	AAP feeding changed the intestinal environment in the mice.
31284990	3	55	theme	AAP	477:479	arg1	feeding					481:487	AAP feeding	477:487	AAP feeding	477:487	AAP feeding changed the intestinal environment in the mice.
31284990	5	56	from	decrease	878:885	arg1	ratio					919:923	the Firmicutes/Bacteroidetes ratio	890:923	the Firmicutes/Bacteroidetes ratio (P < .05)	890:933	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	5	56	from	decrease	878:885	arg1	P < .05					926:932	P < .05	926:932	P < .05	926:932	High throughput sequencing revealed an enrichment in the diversity and an alteration in the composition of the fecal microbiota in the AAP fed mice with a significant decrease in the Firmicutes/Bacteroidetes ratio (P < .05).
31284990	0	57	theme	intestinal	72:81	arg1	microbiota					83:92	the intestinal microbiota	68:92	the intestinal microbiota in mice	68:100	Consumption of polysaccharides from Auricularia auricular modulates the intestinal microbiota in mice.
31284990	6	58	theme	IgA	1090:1092	arg1	P < .05					1118:1124	P < .05	1118:1124	P < .05	1118:1124	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	6	58	theme	IgA	1090:1092	arg1	concentrations					1102:1115	serum IgA and IgG concentrations	1084:1115	serum IgA and IgG concentrations (P < .05)	1084:1125	The relative abundances of Porphyromonadaceae and Bacteroidaceae also increased in the AAP fed mice which positively correlated with an increase in serum IgA and IgG concentrations (P < .05).
31284990	4	59	theme	compartments	568:579	arg1	value					544:548	The pH value	537:548	The pH value of the intestinal compartments	537:579	The pH value of the intestinal compartments decreased while SCFAs concentrations increased in AAP-fed groups, in a dose dependent manner, compared to the controls (P < .05).
31284990	1	60	theme	A.	154:155	arg1	AAP					184:186	AAP	184:186	AAP	184:186	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	60	theme	A.	154:155	arg1	polysaccharides					167:181	A. auricular polysaccharides	154:181	A. auricular polysaccharides (AAP)	154:187	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
31284990	1	61	theme	intestinal	240:249	arg1	microbe					251:257	the intestinal microbe	236:257	the intestinal microbe in mice	236:265	The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice.
29680901	11	0	theme	alkyl	1865:1869	arg1	synthesis					1881:1889	alkyl glycoside synthesis	1865:1889	alkyl glycoside synthesis	1865:1889	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	2	1	theme	alkyl	368:372	arg1	glycosides					374:383	alkyl glycosides	368:383	alkyl glycosides	368:383	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	2	1	theme	alkyl	368:372	arg1	surfactants					400:410	biodegradable surfactants	386:410	biodegradable surfactants	386:410	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	10	2	theme	surfactant	1593:1602	arg1	properties					1604:1613	The surfactant properties	1589:1613	The surfactant properties of the synthesized hexyl mannooligosides	1589:1654	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	5	3	theme	mannooligosides	958:972	arg1	capacity					905:912	alcoholysis capacity	893:912	alcoholysis capacity	893:912	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	5	3	theme	mannooligosides	958:972	arg1	hydrolysis					938:947	potential secondary hydrolysis	918:947	potential secondary hydrolysis	918:947	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	3	4	theme	family	534:539	arg1	TrMan5A-R171K					603:615	TrMan5A-R171K	603:615	TrMan5A-R171K	603:615	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	4	theme	family	534:539	arg1	TrMan5A					591:597	TrMan5A	591:597	TrMan5A	591:597	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	4	theme	family	534:539	arg1	β-mannanases					543:554	glycoside hydrolase family 5 β-mannanases	514:554	glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K)	514:616	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	6	5	theme	hexyl	1174:1178	arg1	mannooligosides					1180:1194	hexyl mannooligosides	1174:1194	hexyl mannooligosides with 1-hexanol	1174:1209	Among the three β-mannanases studied, TrMan5A was the most efficient in producing hexyl mannooligosides with 1-hexanol as acceptor.
29680901	4	6	theme	products	821:828	arg1	formation					796:804	the formation	792:804	the formation of alcoholysis products using different enzymes or reaction conditions	792:875	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	3	7	theme	fungi	565:569	arg1	reesei					583:588	the fungi Trichoderma reesei	561:588	the fungi Trichoderma reesei	561:588	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	9	8	theme	predicted	1563:1571	arg1	β-conformation					1573:1586	their predicted β-conformation	1557:1586	their predicted β-conformation	1557:1586	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	11	9	theme	glycoside	1871:1879	arg1	synthesis					1881:1889	alkyl glycoside synthesis	1865:1889	alkyl glycoside synthesis	1865:1889	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	10	10	contain	have	1708:1711	arg1	they					1703:1706	they	1703:1706	they	1703:1706	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	10	contain	have	1708:1711	arg2	properties					1737:1746	similar micelle-forming properties	1713:1746	similar micelle-forming properties	1713:1746	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	10	contain	have	1708:1711	arg2	glucosides					1780:1789	commercially available hexyl glucosides	1751:1789	commercially available hexyl glucosides	1751:1789	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	3	11	theme	Trichoderma	571:581	arg1	reesei					583:588	the fungi Trichoderma reesei	561:588	the fungi Trichoderma reesei	561:588	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	9	12	theme	magnetic	1506:1513	arg1	resonance					1515:1523	nuclear magnetic resonance	1498:1523	nuclear magnetic resonance	1498:1523	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	3	13	theme	mannooligosides	492:506	arg1	synthesis					473:481	the synthesis	469:481	the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C)	469:651	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	4	14	theme	spectrometry-based	713:730	arg1	method					732:737	a novel mass spectrometry-based method	700:737	a novel mass spectrometry-based method	700:737	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	9	15	theme	synthesized	1426:1436	arg1	mannooligosides					1444:1458	The synthesized hexyl mannooligosides	1422:1458	The synthesized hexyl mannooligosides	1422:1458	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	10	16	theme	hexyl	1634:1638	arg1	mannooligosides					1640:1654	the synthesized hexyl mannooligosides	1618:1654	the synthesized hexyl mannooligosides	1618:1654	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	4	17	theme	alcoholysis	809:819	arg1	products					821:828	alcoholysis products	809:828	alcoholysis products using different enzymes or reaction conditions	809:875	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	5	18	theme	secondary	928:936	arg1	hydrolysis					938:947	potential secondary hydrolysis	918:947	potential secondary hydrolysis	918:947	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	8	19	theme	high	1356:1359	arg1	selectivity					1361:1371	a high selectivity	1354:1371	a high selectivity of TrMan5A for 1-hexanol	1354:1396	The data suggests a high selectivity of TrMan5A for 1-hexanol as acceptor over water.
29680901	7	20	theme	high-performance	1296:1311	arg1	chromatography					1320:1333	high-performance liquid chromatography	1296:1333	high-performance liquid chromatography	1296:1333	Hexyl mannooligosides were synthesized using TrMan5A and purified using high-performance liquid chromatography.
29680901	9	21	theme	hexyl	1438:1442	arg1	mannooligosides					1444:1458	The synthesized hexyl mannooligosides	1422:1458	The synthesized hexyl mannooligosides	1422:1458	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	10	22	theme	synthesized	1622:1632	arg1	mannooligosides					1640:1654	the synthesized hexyl mannooligosides	1618:1654	the synthesized hexyl mannooligosides	1618:1654	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	2	23	theme	glycosyl	283:290	arg1	alcoholysis					303:313	alcoholysis	303:313	alcoholysis	303:313	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	2	23	theme	glycosyl	283:290	arg1	alcohols					271:278	alcohols	271:278	alcohols	271:278	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	2	23	theme	glycosyl	283:290	arg1	acceptors					292:300	glycosyl acceptors	283:300	glycosyl acceptors (alcoholysis)	283:314	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	4	24	theme	alcoholysis	678:688	arg1	capacity					690:697	β-mannanase alcoholysis capacity	666:697	β-mannanase alcoholysis capacity	666:697	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	6	25	with	mannooligosides	1180:1194	arg1	1-hexanol					1201:1209	1-hexanol	1201:1209	1-hexanol	1201:1209	Among the three β-mannanases studied, TrMan5A was the most efficient in producing hexyl mannooligosides with 1-hexanol as acceptor.
29680901	4	26	theme	relative	769:776	arg1	comparison					778:787	relative comparison	769:787	relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions	769:875	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	1	27	theme	transglycosylation	180:197	arg1	capable					169:175	capable	169:175	capable	169:175	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	0	28	theme	β-Mannanase-catalyzed	0:20	arg1	synthesis					22:30	β-Mannanase-catalyzed synthesis	0:30	β-Mannanase-catalyzed synthesis of alkyl mannooligosides	0:55	β-Mannanase-catalyzed synthesis of alkyl mannooligosides.
29680901	2	29	used	used	349:352	arg2	β-mannanases					317:328	β-mannanases	317:328	β-mannanases	317:328	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	1	30	theme	β-mannans	115:123	arg1	modification					99:110	modification	99:110	modification	99:110	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	1	30	theme	β-mannans	115:123	arg1	conversion					84:93	conversion	84:93	conversion	84:93	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	2	31	theme	biodegradable	386:398	arg1	glycosides					374:383	alkyl glycosides	368:383	alkyl glycosides	368:383	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	2	31	theme	biodegradable	386:398	arg1	surfactants					400:410	biodegradable surfactants	386:410	biodegradable surfactants	386:410	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	0	32	theme	alkyl	35:39	arg1	mannooligosides					41:55	alkyl mannooligosides	35:55	alkyl mannooligosides	35:55	β-Mannanase-catalyzed synthesis of alkyl mannooligosides.
29680901	9	33	with	resonance	1515:1523	arg1	results					1531:1537	results	1531:1537	results in agreement with their predicted β-conformation	1531:1586	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	10	34	theme	hexyl	1774:1778	arg1	glucosides					1780:1789	commercially available hexyl glucosides	1751:1789	commercially available hexyl glucosides	1751:1789	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	34	theme	hexyl	1774:1778	arg1	properties					1737:1746	similar micelle-forming properties	1713:1746	similar micelle-forming properties	1713:1746	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	3	35	dep	β-mannanases	543:554	arg1	TrMan5A-R171K					603:615	TrMan5A-R171K	603:615	TrMan5A-R171K	603:615	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	35	dep	β-mannanases	543:554	arg1	TrMan5A					591:597	TrMan5A	591:597	TrMan5A	591:597	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	35	dep	β-mannanases	543:554	arg1	β-mannanases					543:554	glycoside hydrolase family 5 β-mannanases	514:554	glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K)	514:616	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	8	36	theme	TrMan5A	1376:1382	arg1	selectivity					1361:1371	a high selectivity	1354:1371	a high selectivity of TrMan5A for 1-hexanol	1354:1396	The data suggests a high selectivity of TrMan5A for 1-hexanol as acceptor over water.
29680901	3	37	theme	hydrolase	524:532	arg1	TrMan5A-R171K					603:615	TrMan5A-R171K	603:615	TrMan5A-R171K	603:615	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	37	theme	hydrolase	524:532	arg1	TrMan5A					591:597	TrMan5A	591:597	TrMan5A	591:597	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	37	theme	hydrolase	524:532	arg1	β-mannanases					543:554	glycoside hydrolase family 5 β-mannanases	514:554	glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K)	514:616	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	5	38	theme	alcoholysis	893:903	arg1	capacity					905:912	alcoholysis capacity	893:912	alcoholysis capacity	893:912	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	4	39	theme	reaction	857:864	arg1	conditions					866:875	reaction conditions	857:875	reaction conditions	857:875	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	4	40	theme	β-mannanase	666:676	arg1	capacity					690:697	β-mannanase alcoholysis capacity	666:697	β-mannanase alcoholysis capacity	666:697	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	0	41	theme	mannooligosides	41:55	arg1	synthesis					22:30	β-Mannanase-catalyzed synthesis	0:30	β-Mannanase-catalyzed synthesis of alkyl mannooligosides	0:55	β-Mannanase-catalyzed synthesis of alkyl mannooligosides.
29680901	9	42	theme	nuclear	1498:1504	arg1	resonance					1515:1523	nuclear magnetic resonance	1498:1523	nuclear magnetic resonance	1498:1523	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	10	43	theme	mannooligosides	1640:1654	arg1	properties					1604:1613	The surfactant properties	1589:1613	The surfactant properties of the synthesized hexyl mannooligosides	1589:1654	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	3	44	theme	Aspergillus	622:632	arg1	AnMan5C					644:650	AnMan5C	644:650	AnMan5C	644:650	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	44	theme	Aspergillus	622:632	arg1	nidulans					634:641	Aspergillus nidulans	622:641	Aspergillus nidulans (AnMan5C)	622:651	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	1	45	theme	enzymatic	219:227	arg1	synthesis					229:237	enzymatic synthesis	219:237	enzymatic synthesis of novel glycoconjugates	219:262	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	7	46	theme	Hexyl	1224:1228	arg1	mannooligosides					1230:1244	Hexyl mannooligosides	1224:1244	Hexyl mannooligosides	1224:1244	Hexyl mannooligosides were synthesized using TrMan5A and purified using high-performance liquid chromatography.
29680901	7	47	theme	liquid	1313:1318	arg1	chromatography					1320:1333	high-performance liquid chromatography	1296:1333	high-performance liquid chromatography	1296:1333	Hexyl mannooligosides were synthesized using TrMan5A and purified using high-performance liquid chromatography.
29680901	10	48	theme	available	1764:1772	arg1	glucosides					1780:1789	commercially available hexyl glucosides	1751:1789	commercially available hexyl glucosides	1751:1789	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	48	theme	available	1764:1772	arg1	properties					1737:1746	similar micelle-forming properties	1713:1746	similar micelle-forming properties	1713:1746	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	11	49	theme	renewable	1934:1942	arg1	β-mannans					1944:1952	renewable β-mannans	1934:1952	renewable β-mannans	1934:1952	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	5	50	from	Differences	878:888	arg1	capacity					905:912	alcoholysis capacity	893:912	alcoholysis capacity	893:912	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	5	50	from	Differences	878:888	arg1	hydrolysis					938:947	potential secondary hydrolysis	918:947	potential secondary hydrolysis	918:947	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	3	51	theme	glycoside	514:522	arg1	TrMan5A-R171K					603:615	TrMan5A-R171K	603:615	TrMan5A-R171K	603:615	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	51	theme	glycoside	514:522	arg1	TrMan5A					591:597	TrMan5A	591:597	TrMan5A	591:597	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	51	theme	glycoside	514:522	arg1	β-mannanases					543:554	glycoside hydrolase family 5 β-mannanases	514:554	glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K)	514:616	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	52	from	reesei	583:588	arg1	AnMan5C					644:650	AnMan5C	644:650	AnMan5C	644:650	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	52	from	reesei	583:588	arg1	TrMan5A-R171K					603:615	TrMan5A-R171K	603:615	TrMan5A-R171K	603:615	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	52	from	reesei	583:588	arg1	TrMan5A					591:597	TrMan5A	591:597	TrMan5A	591:597	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	52	from	reesei	583:588	arg1	nidulans					634:641	Aspergillus nidulans	622:641	Aspergillus nidulans (AnMan5C)	622:651	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	3	52	from	reesei	583:588	arg1	β-mannanases					543:554	glycoside hydrolase family 5 β-mannanases	514:554	glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K)	514:616	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	11	53	theme	potential	1909:1917	arg1	utilization					1919:1929	the potential utilization	1905:1929	the potential utilization of renewable β-mannans	1905:1952	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	11	54	theme	β-mannans	1944:1952	arg1	utilization					1919:1929	the potential utilization	1905:1929	the potential utilization of renewable β-mannans	1905:1952	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	3	55	theme	alkyl	486:490	arg1	mannooligosides					492:506	alkyl mannooligosides	486:506	alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C)	486:651	In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C).
29680901	9	56	from	results	1531:1537	arg1	agreement					1542:1550	agreement	1542:1550	agreement with their predicted β-conformation	1542:1586	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	4	57	theme	formation	796:804	arg1	comparison					778:787	relative comparison	769:787	relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions	769:875	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	1	58	dep	conversion	84:93	arg1	the					80:82	the	80:82	the	80:82	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	10	59	theme	micelle-forming	1721:1735	arg1	glucosides					1780:1789	commercially available hexyl glucosides	1751:1789	commercially available hexyl glucosides	1751:1789	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	59	theme	micelle-forming	1721:1735	arg1	properties					1737:1746	similar micelle-forming properties	1713:1746	similar micelle-forming properties	1713:1746	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	2	60	theme	renewable	418:426	arg1	β-mannans					428:436	renewable β-mannans	418:436	renewable β-mannans	418:436	Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans.
29680901	9	61	with	agreement	1542:1550	arg1	β-conformation					1573:1586	their predicted β-conformation	1557:1586	their predicted β-conformation	1557:1586	The synthesized hexyl mannooligosides were structurally characterized using nuclear magnetic resonance, with results in agreement with their predicted β-conformation.
29680901	5	62	theme	alkyl	952:956	arg1	mannooligosides					958:972	alkyl mannooligosides	952:972	alkyl mannooligosides	952:972	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	5	63	theme	potential	918:926	arg1	hydrolysis					938:947	potential secondary hydrolysis	918:947	potential secondary hydrolysis	918:947	Differences in alcoholysis capacity and potential secondary hydrolysis of alkyl mannooligosides were observed when comparing alcoholysis catalyzed by the three β-mannanases using methanol or 1-hexanol as acceptor.
29680901	1	64	theme	novel	242:246	arg1	glycoconjugates					248:262	novel glycoconjugates	242:262	novel glycoconjugates	242:262	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
29680901	10	65	theme	similar	1713:1719	arg1	glucosides					1780:1789	commercially available hexyl glucosides	1751:1789	commercially available hexyl glucosides	1751:1789	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	10	65	theme	similar	1713:1719	arg1	properties					1737:1746	similar micelle-forming properties	1713:1746	similar micelle-forming properties	1713:1746	The surfactant properties of the synthesized hexyl mannooligosides were evaluated using tensiometry, showing that they have similar micelle-forming properties as commercially available hexyl glucosides.
29680901	4	66	theme	different	836:844	arg1	enzymes					846:852	different enzymes	836:852	different enzymes	836:852	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	11	67	theme	present	1796:1802	arg1	paper					1804:1808	The present paper	1792:1808	The present paper	1792:1808	The present paper demonstrates the possibility of using β-mannanases for alkyl glycoside synthesis and increases the potential utilization of renewable β-mannans.
29680901	4	68	theme	novel	702:706	arg1	method					732:737	a novel mass spectrometry-based method	700:737	a novel mass spectrometry-based method	700:737	To evaluate β-mannanase alcoholysis capacity, a novel mass spectrometry-based method was developed that allows for relative comparison of the formation of alcoholysis products using different enzymes or reaction conditions.
29680901	1	69	theme	glycoconjugates	248:262	arg1	synthesis					229:237	enzymatic synthesis	219:237	enzymatic synthesis of novel glycoconjugates	219:262	β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates.
31852774	5	0	theme	known	732:736	arg1	interaction					703:713	interaction	703:713	interaction	703:713	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	5	0	theme	known	732:736	arg1	hierarchy					719:727	hierarchy	719:727	hierarchy	719:727	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	8	1	theme	folding	1349:1355	arg1	status					1357:1362	folding status	1349:1362	folding status	1349:1362	Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status.
31852774	5	2	dep	interaction	703:713	arg1	the					699:701	the	699:701	the	699:701	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	0	3	theme	Subcellular	64:74	arg1	Dynamics					76:83	Subcellular Dynamics	64:83	Subcellular Dynamics	64:83	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	6	4	theme	steady-state	875:886	arg1	localization					888:899	Conventional steady-state localization	862:899	Conventional steady-state localization	862:899	Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts.
31852774	8	5	theme	checkpoints/branches	1231:1250	arg1	presence					1210:1217	the presence	1206:1217	the presence of multiple checkpoints/branches	1206:1250	Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status.
31852774	4	6	theme	host	661:664	arg1	genotypes					666:674	host genotypes	661:674	host genotypes	661:674	These processes are governed by multiple sequence motifs and also host genotypes.
31852774	8	7	theme	KOR1	1259:1262	arg1	trafficking					1264:1274	KOR1 trafficking	1259:1274	KOR1 trafficking	1259:1274	Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status.
31852774	0	8	theme	Salt-Stress	89:99	arg1	Function					111:118	Salt-Stress Tolerance Function	89:118	Salt-Stress Tolerance Function of KORRIGAN1	89:131	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	6	9	theme	Conventional	862:873	arg1	localization					888:899	Conventional steady-state localization	862:899	Conventional steady-state localization	862:899	Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts.
31852774	9	10	theme	dominant	1394:1401	arg1	variants					1434:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	1	11	theme	cellulose	265:273	arg1	biosynthesis					275:286	cellulose biosynthesis	265:286	cellulose biosynthesis	265:286	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	1	12	theme	abiotic	358:364	arg1	tolerance					373:381	abiotic stress tolerance	358:381	abiotic stress tolerance	358:381	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	9	13	theme	stress	1557:1562	arg1	acclimation					1564:1574	stress acclimation	1557:1574	stress acclimation	1557:1574	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	9	14	theme	active	1470:1475	arg1	removal					1477:1483	active removal	1470:1483	active removal of KOR1 from the PM	1470:1503	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	1	15	theme	stress	366:371	arg1	tolerance					373:381	abiotic stress tolerance	358:381	abiotic stress tolerance	358:381	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	7	16	theme	KOR1	1092:1095	arg1	proteins					1097:1104	young versus aged KOR1 proteins	1074:1104	young versus aged KOR1 proteins	1074:1104	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	1	17	theme	plant	170:174	arg1	system					186:191	the plant secretory system	166:191	the plant secretory system	166:191	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	0	18	theme	Tolerance	101:109	arg1	Function					111:118	Salt-Stress Tolerance Function	89:118	Salt-Stress Tolerance Function of KORRIGAN1	89:131	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	2	19	dep	cycles	437:442	arg1	insertion					390:398	insertion	390:398	insertion into the endoplasmic reticulum	390:429	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	1	20	theme	secretory	176:184	arg1	system					186:191	the plant secretory system	166:191	the plant secretory system	166:191	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	0	21	theme	Quality	9:15	arg1	Mechanisms					25:34	Multiple Quality Control Mechanisms	0:34	Multiple Quality Control Mechanisms in the ER and TGN	0:52	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	9	22	theme	KOR1	1488:1491	arg1	removal					1477:1483	active removal	1470:1483	active removal of KOR1 from the PM	1470:1503	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	0	23	theme	Multiple	0:7	arg1	Mechanisms					25:34	Multiple Quality Control Mechanisms	0:34	Multiple Quality Control Mechanisms in the ER and TGN	0:52	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	7	24	theme	aged	1087:1090	arg1	proteins					1097:1104	young versus aged KOR1 proteins	1074:1104	young versus aged KOR1 proteins	1074:1104	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	4	25	theme	sequence	636:643	arg1	motifs					645:650	multiple sequence motifs	627:650	multiple sequence motifs	627:650	These processes are governed by multiple sequence motifs and also host genotypes.
31852774	0	26	theme	KORRIGAN1	123:131	arg1	Dynamics					76:83	Subcellular Dynamics	64:83	Subcellular Dynamics	64:83	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	0	26	theme	KORRIGAN1	123:131	arg1	Function					111:118	Salt-Stress Tolerance Function	89:118	Salt-Stress Tolerance Function of KORRIGAN1	89:131	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	9	27	theme	removal	1477:1483	arg1	importance					1456:1465	the importance	1452:1465	the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation	1452:1574	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	8	28	theme	multiple	1222:1229	arg1	checkpoints/branches					1231:1250	multiple checkpoints/branches	1222:1250	multiple checkpoints/branches	1222:1250	Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status.
31852774	7	29	theme	proteins	1097:1104	arg1	visualization					1057:1069	differential visualization	1044:1069	differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway	1044:1182	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	2	30	theme	KOR1	432:435	arg1	cycles					437:442	KOR1 cycles	432:442	KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM)	432:509	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	0	31	theme	Control	17:23	arg1	Mechanisms					25:34	Multiple Quality Control Mechanisms	0:34	Multiple Quality Control Mechanisms in the ER and TGN	0:52	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	7	32	theme	young	1074:1078	arg1	proteins					1097:1104	young versus aged KOR1 proteins	1074:1104	young versus aged KOR1 proteins	1074:1104	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	5	33	from	interaction	703:713	arg1	KOR1					778:781	KOR1	778:781	KOR1	778:781	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	7	34	dep	proteins	1097:1104	arg1	enabling					1107:1114	enabling	1107:1114	enabling the analysis of single-pass transport through the secretory pathway	1107:1182	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	7	35	theme	transport	1144:1152	arg1	analysis					1120:1127	the analysis	1116:1127	the analysis of single-pass transport	1116:1152	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	7	36	theme	differential	1044:1055	arg1	visualization					1057:1069	differential visualization	1044:1069	differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway	1044:1182	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	3	37	theme	cell	561:564	arg1	plates					566:571	growing cell plates	553:571	growing cell plates	553:571	From the TGN, the protein is targeted to growing cell plates during cell division.
31852774	7	38	theme	timer	1015:1019	arg1	technology					1021:1030	a tandem fluorescent timer technology	994:1030	a tandem fluorescent timer technology	994:1030	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	5	39	theme	KOR1	803:806	arg1	transport					808:816	KOR1 transport	803:816	KOR1 transport	803:816	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	4	40	theme	multiple	627:634	arg1	motifs					645:650	multiple sequence motifs	627:650	multiple sequence motifs	627:650	These processes are governed by multiple sequence motifs and also host genotypes.
31852774	6	41	theme	KOR1	938:941	arg1	variants					943:950	structurally compromised KOR1 variants	913:950	structurally compromised KOR1 variants	913:950	Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts.
31852774	5	42	theme	identified	748:757	arg1	signals					767:773	newly identified sorting signals	742:773	newly identified sorting signals	742:773	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	9	43	theme	variants	1434:1441	arg1	analyses					1382:1389	growth analyses	1375:1389	growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants	1375:1441	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	7	44	theme	tandem	996:1001	arg1	technology					1021:1030	a tandem fluorescent timer technology	994:1030	a tandem fluorescent timer technology	994:1030	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	2	45	theme	endoplasmic	409:419	arg1	reticulum					421:429	the endoplasmic reticulum	405:429	the endoplasmic reticulum	405:429	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	5	46	theme	sorting	759:765	arg1	signals					767:773	newly identified sorting signals	742:773	newly identified sorting signals	742:773	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	6	47	theme	compromised	926:936	arg1	variants					943:950	structurally compromised KOR1 variants	913:950	structurally compromised KOR1 variants	913:950	Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts.
31852774	1	48	gly	glycoproteins	145:157	arg1	glycoproteins					145:157	many glycoproteins	140:157	many glycoproteins within the plant secretory system	140:191	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	7	49	theme	fluorescent	1003:1013	arg1	technology					1021:1030	a tandem fluorescent timer technology	994:1030	a tandem fluorescent timer technology	994:1030	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	8	50	theme	sequence	1329:1336	arg1	motifs					1338:1343	KOR1's sequence motifs	1322:1343	KOR1's sequence motifs	1322:1343	Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status.
31852774	5	51	theme	signals	767:773	arg1	interaction					703:713	interaction	703:713	interaction	703:713	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	5	51	theme	signals	767:773	arg1	hierarchy					719:727	hierarchy	719:727	hierarchy	719:727	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	0	52	from	Mechanisms	25:34	arg1	TGN					50:52	TGN	50:52	TGN	50:52	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	0	52	from	Mechanisms	25:34	arg1	ER					43:44	ER	43:44	ER	43:44	Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.
31852774	9	53	from	importance	1456:1465	arg1	PM					1502:1503	the PM	1498:1503	the PM	1498:1503	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	3	54	theme	growing	553:559	arg1	plates					566:571	growing cell plates	553:571	growing cell plates	553:571	From the TGN, the protein is targeted to growing cell plates during cell division.
31852774	7	55	theme	secretory	1166:1174	arg1	pathway					1176:1182	the secretory pathway	1162:1182	the secretory pathway	1162:1182	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	1	56	theme	cell	330:333	arg1	biosynthesis					340:351	cell wall biosynthesis	330:351	cell wall biosynthesis	330:351	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	5	57	dep	investigated	686:697	arg1	affect					796:801	affect	796:801	affect KOR1 transport at various stages in the secretory pathway	796:859	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	7	58	theme	single-pass	1132:1142	arg1	transport					1144:1152	single-pass transport	1132:1152	single-pass transport	1132:1152	In addition, a tandem fluorescent timer technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway.
31852774	9	59	from	PM	1502:1503	arg1	importance					1456:1465	the importance	1452:1465	the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation	1452:1574	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	9	59	from	PM	1502:1503	arg1	removal					1477:1483	active removal	1470:1483	active removal of KOR1 from the PM	1470:1503	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	9	60	theme	PM-confined	1403:1413	arg1	variants					1434:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	5	61	from	hierarchy	719:727	arg1	KOR1					778:781	KOR1	778:781	KOR1	778:781	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	5	62	from	stages	829:834	arg1	pathway					853:859	the secretory pathway	839:859	the secretory pathway	839:859	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	5	63	theme	secretory	843:851	arg1	pathway					853:859	the secretory pathway	839:859	the secretory pathway	839:859	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	9	64	theme	growth	1375:1380	arg1	analyses					1382:1389	growth analyses	1375:1389	growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants	1375:1441	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	9	65	theme	KOR1-L48L49→A48A49	1415:1432	arg1	variants					1434:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	dominant PM-confined KOR1-L48L49→A48A49 variants	1394:1441	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	9	66	theme	salt	1512:1515	arg1	stress					1517:1522	salt stress	1512:1522	salt stress	1512:1522	Moreover, growth analyses of dominant PM-confined KOR1-L48L49→A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.
31852774	1	67	theme	many	140:143	arg1	glycoproteins					145:157	many glycoproteins	140:157	many glycoproteins within the plant secretory system	140:191	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	5	68	theme	various	821:827	arg1	stages					829:834	various stages	821:834	various stages in the secretory pathway	821:859	Here, we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1 and how they affect KOR1 transport at various stages in the secretory pathway.
31852774	1	69	theme	membrane-anchored	214:230	arg1	endo-β-1,4-glucanase					232:251	a membrane-anchored endo-β-1,4-glucanase	212:251	a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis	212:286	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	1	69	theme	membrane-anchored	214:230	arg1	KORRIGAN1					194:202	KORRIGAN1	194:202	KORRIGAN1 (KOR1)	194:209	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	2	70	theme	plasma	490:495	arg1	PM					507:508	PM	507:508	PM	507:508	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	2	70	theme	plasma	490:495	arg1	membrane					497:504	the plasma membrane	486:504	the plasma membrane (PM)	486:509	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	1	71	theme	wall	335:338	arg1	biosynthesis					340:351	cell wall biosynthesis	330:351	cell wall biosynthesis	330:351	Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-β-1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell wall biosynthesis, and abiotic stress tolerance.
31852774	3	72	theme	cell	580:583	arg1	division					585:592	cell division	580:592	cell division	580:592	From the TGN, the protein is targeted to growing cell plates during cell division.
31852774	2	73	theme	trans-Golgi	456:466	arg1	TGN					477:479	TGN	477:479	TGN	477:479	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
31852774	2	73	theme	trans-Golgi	456:466	arg1	network					468:474	the trans-Golgi network	452:474	the trans-Golgi network (TGN)	452:480	After insertion into the endoplasmic reticulum, KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM).
30024928	0	0	theme	composites	92:101	arg1	performance					35:45	the heat conduction performance	15:45	the heat conduction performance of boron nitride/cellulosic fibre insulating composites	15:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	4	1	theme	BN/cellulosic	599:611	arg1	composites					619:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	This work provided a method to prepare thermally conductive and electrically insulating BN/cellulosic fibre composites.
30024928	1	2	theme	power	173:177	arg1	output					179:184	high power output	168:184	high power output	168:184	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	9	3	theme	insulating	1125:1134	arg1	material					1136:1143	The resultant insulating material	1111:1143	The resultant insulating material which has better heat conduction property	1111:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	0	4	theme	insulating	81:90	arg1	composites					92:101	boron nitride/cellulosic fibre insulating composites	50:101	boron nitride/cellulosic fibre insulating composites	50:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	9	5	from	development	1224:1234	arg1	equipment					1250:1258	electrical equipment	1239:1258	electrical equipment	1239:1258	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	9	6	contain	has	1151:1153	arg1	material					1136:1143	The resultant insulating material	1111:1143	The resultant insulating material which has better heat conduction property	1111:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	9	6	contain	has	1151:1153	arg2	property					1178:1185	better heat conduction property	1155:1185	better heat conduction property	1155:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	2	7	theme	poor	334:337	arg1	conductivity					347:358	poor thermal conductivity	334:358	poor thermal conductivity	334:358	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	3	8	theme	heat	482:485	arg1	performance					498:508	heat conduction performance	482:508	heat conduction performance	482:508	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	3	9	theme	cellulose-based	434:448	arg1	material					461:468	cellulose-based insulating material	434:468	cellulose-based insulating material	434:468	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	7	10	theme	composites	951:960	arg1	properties					923:932	the insulating properties	908:932	the insulating properties of the resultant composites	908:960	Simultaneously, BN fillers improved the insulating properties of the resultant composites.
30024928	9	11	theme	conduction	1167:1176	arg1	property					1178:1185	better heat conduction property	1155:1185	better heat conduction property	1155:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	3	12	theme	insulating	411:420	arg1	filler					422:427	thermally conductive and electrically insulating filler	373:427	thermally conductive and electrically insulating filler	373:427	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	4	13	theme	conductive	560:569	arg1	composites					619:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	This work provided a method to prepare thermally conductive and electrically insulating BN/cellulosic fibre composites.
30024928	5	14	theme	dual-sized	733:742	arg1	fillers					744:750	dual-sized fillers	733:750	dual-sized fillers	733:750	And the thermal conductivity of the composites was remarkably increased via grafting APTES and adding dual-sized fillers.
30024928	9	15	theme	better	1155:1160	arg1	property					1178:1185	better heat conduction property	1155:1185	better heat conduction property	1155:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	9	16	theme	heat	1162:1165	arg1	property					1178:1185	better heat conduction property	1155:1185	better heat conduction property	1155:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	1	17	theme	better	195:200	arg1	performance					219:229	better heat dissipation performance	195:229	better heat dissipation performance of internal insulation structure	195:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	3	18	theme	conductive	383:392	arg1	filler					422:427	thermally conductive and electrically insulating filler	373:427	thermally conductive and electrically insulating filler	373:427	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	7	19	theme	resultant	941:949	arg1	composites					951:960	the resultant composites	937:960	the resultant composites	937:960	Simultaneously, BN fillers improved the insulating properties of the resultant composites.
30024928	1	20	theme	heat	202:205	arg1	performance					219:229	better heat dissipation performance	195:229	better heat dissipation performance of internal insulation structure	195:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	6	21	theme	%	869:869	arg1	loading					850:856	h-BN loading	845:856	h-BN loading of 41.08 wt%	845:869	The thermal conductivity of the composite reached 0.682 W/(m•k) that increased by 387% with h-BN loading of 41.08 wt%.
30024928	1	22	theme	dissipation	207:217	arg1	performance					219:229	better heat dissipation performance	195:229	better heat dissipation performance of internal insulation structure	195:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	0	23	theme	heat	19:22	arg1	performance					35:45	the heat conduction performance	15:45	the heat conduction performance of boron nitride/cellulosic fibre insulating composites	15:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	6	24	theme	wt	867:868	arg1	%					869:869	41.08 wt%	861:869	41.08 wt%	861:869	The thermal conductivity of the composite reached 0.682 W/(m•k) that increased by 387% with h-BN loading of 41.08 wt%.
30024928	1	25	theme	continuous	108:117	arg1	development					119:129	The continuous development	104:129	The continuous development of high electrical equipment towards high power output	104:184	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	8	26	theme	breaking	988:995	arg1	strength					997:1004	breaking strength	988:1004	breaking strength of	988:1007	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	2	27	with	challenges	268:277	arg1	conductivity					347:358	poor thermal conductivity	334:358	poor thermal conductivity	334:358	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	2	28	theme	thermal	339:345	arg1	conductivity					347:358	poor thermal conductivity	334:358	poor thermal conductivity	334:358	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	9	29	theme	resultant	1115:1123	arg1	material					1136:1143	The resultant insulating material	1111:1143	The resultant insulating material which has better heat conduction property	1111:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	0	30	theme	performance	35:45	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.	0:102	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	7	31	theme	BN	888:889	arg1	fillers					891:897	BN fillers	888:897	BN fillers	888:897	Simultaneously, BN fillers improved the insulating properties of the resultant composites.
30024928	7	32	theme	insulating	912:921	arg1	properties					923:932	the insulating properties	908:932	the insulating properties of the resultant composites	908:960	Simultaneously, BN fillers improved the insulating properties of the resultant composites.
30024928	1	33	theme	internal	234:241	arg1	structure					254:262	internal insulation structure	234:262	internal insulation structure	234:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	0	34	theme	conduction	24:33	arg1	performance					35:45	the heat conduction performance	15:45	the heat conduction performance of boron nitride/cellulosic fibre insulating composites	15:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	3	35	theme	insulating	450:459	arg1	material					461:468	cellulose-based insulating material	434:468	cellulose-based insulating material	434:468	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	9	36	theme	vast	1198:1201	arg1	potential					1203:1211	a vast potential	1196:1211	a vast potential for future development in electrical equipment	1196:1258	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	1	37	theme	insulation	243:252	arg1	structure					254:262	internal insulation structure	234:262	internal insulation structure	234:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	0	38	theme	boron	50:54	arg1	composites					92:101	boron nitride/cellulosic fibre insulating composites	50:101	boron nitride/cellulosic fibre insulating composites	50:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	3	39	theme	electrically	398:409	arg1	filler					422:427	thermally conductive and electrically insulating filler	373:427	thermally conductive and electrically insulating filler	373:427	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	8	40	theme	volume	1013:1018	arg1	resistivity					1020:1030	volume resistivity	1013:1030	volume resistivity of the composites	1013:1048	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	3	41	theme	conduction	487:496	arg1	performance					498:508	heat conduction performance	482:508	heat conduction performance	482:508	Introducing thermally conductive and electrically insulating filler into cellulose-based insulating material can enhance heat conduction performance.
30024928	9	42	contain	have	1191:1194	arg1	material					1136:1143	The resultant insulating material	1111:1143	The resultant insulating material which has better heat conduction property	1111:1185	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	9	42	contain	have	1191:1194	arg2	potential					1203:1211	a vast potential	1196:1211	a vast potential for future development in electrical equipment	1196:1258	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	5	43	theme	composites	667:676	arg1	conductivity					647:658	the thermal conductivity	635:658	the thermal conductivity of the composites	635:676	And the thermal conductivity of the composites was remarkably increased via grafting APTES and adding dual-sized fillers.
30024928	1	44	theme	high	134:137	arg1	equipment					150:158	high electrical equipment	134:158	high electrical equipment	134:158	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	6	45	theme	composite	785:793	arg1	conductivity					765:776	The thermal conductivity	753:776	The thermal conductivity of the composite	753:793	The thermal conductivity of the composite reached 0.682 W/(m•k) that increased by 387% with h-BN loading of 41.08 wt%.
30024928	2	46	theme	insulating	307:316	arg1	materials					318:326	the traditional paper-based insulating materials	279:326	the traditional paper-based insulating materials	279:326	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	6	47	theme	h-BN	845:848	arg1	loading					850:856	h-BN loading	845:856	h-BN loading of 41.08 wt%	845:869	The thermal conductivity of the composite reached 0.682 W/(m•k) that increased by 387% with h-BN loading of 41.08 wt%.
30024928	1	48	theme	electrical	139:148	arg1	equipment					150:158	high electrical equipment	134:158	high electrical equipment	134:158	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	9	49	theme	electrical	1239:1248	arg1	equipment					1250:1258	electrical equipment	1239:1258	electrical equipment	1239:1258	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	1	50	theme	structure	254:262	arg1	performance					219:229	better heat dissipation performance	195:229	better heat dissipation performance of internal insulation structure	195:262	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	9	51	theme	future	1217:1222	arg1	development					1224:1234	future development	1217:1234	future development in electrical equipment	1217:1258	The resultant insulating material which has better heat conduction property may have a vast potential for future development in electrical equipment.
30024928	2	52	theme	paper-based	295:305	arg1	materials					318:326	the traditional paper-based insulating materials	279:326	the traditional paper-based insulating materials	279:326	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	1	53	theme	equipment	150:158	arg1	development					119:129	The continuous development	104:129	The continuous development of high electrical equipment towards high power output	104:184	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	0	54	theme	fibre	75:79	arg1	composites					92:101	boron nitride/cellulosic fibre insulating composites	50:101	boron nitride/cellulosic fibre insulating composites	50:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	2	55	theme	traditional	283:293	arg1	materials					318:326	the traditional paper-based insulating materials	279:326	the traditional paper-based insulating materials	279:326	It challenges the traditional paper-based insulating materials, with poor thermal conductivity.
30024928	8	56	theme	composites	1039:1048	arg1	constant					978:985	The dielectric constant	963:985	The dielectric constant	963:985	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	8	56	theme	composites	1039:1048	arg1	strength					997:1004	breaking strength	988:1004	breaking strength of	988:1007	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	8	56	theme	composites	1039:1048	arg1	resistivity					1020:1030	volume resistivity	1013:1030	volume resistivity of the composites	1013:1048	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	8	57	theme	dielectric	967:976	arg1	constant					978:985	The dielectric constant	963:985	The dielectric constant	963:985	The dielectric constant, breaking strength of and volume resistivity of the composites reached 4.75, 9.2 kV/mm-1 and 4.72×10(14) Ω•m, respectively.
30024928	0	58	theme	nitride/cellulosic	56:73	arg1	composites					92:101	boron nitride/cellulosic fibre insulating composites	50:101	boron nitride/cellulosic fibre insulating composites	50:101	Enhancement of the heat conduction performance of boron nitride/cellulosic fibre insulating composites.
30024928	4	59	theme	fibre	613:617	arg1	composites					619:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	thermally conductive and electrically insulating BN/cellulosic fibre composites	550:628	This work provided a method to prepare thermally conductive and electrically insulating BN/cellulosic fibre composites.
30024928	5	60	theme	thermal	639:645	arg1	conductivity					647:658	the thermal conductivity	635:658	the thermal conductivity of the composites	635:676	And the thermal conductivity of the composites was remarkably increased via grafting APTES and adding dual-sized fillers.
30024928	1	61	theme	high	168:171	arg1	output					179:184	high power output	168:184	high power output	168:184	The continuous development of high electrical equipment towards high power output requires better heat dissipation performance of internal insulation structure.
30024928	6	62	theme	thermal	757:763	arg1	conductivity					765:776	The thermal conductivity	753:776	The thermal conductivity of the composite	753:793	The thermal conductivity of the composite reached 0.682 W/(m•k) that increased by 387% with h-BN loading of 41.08 wt%.
29746967	0	0	theme	crosslinking	88:99	arg1	polymerization					101:114	crosslinking polymerization	88:114	crosslinking polymerization	88:114	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	1	1	theme	biomedical	374:383	arg1	applications					385:396	enhanced biomedical applications	365:396	enhanced biomedical applications	365:396	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	1	2	from	applications	201:212	arg1	consideration					249:261	consideration	249:261	consideration	249:261	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	1	3	theme	therapeutic	170:180	arg1	applications					201:212	the therapeutic and pharmaceutical applications	166:212	the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration	166:261	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	6	4	theme	release	1079:1085	arg1	profile					1087:1093	release profile	1079:1093	release profile	1079:1093	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	2	5	dep	oxide	498:502	arg1	GO					505:506	GO	505:506	GO	505:506	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	6	6	theme	kinetic	1131:1137	arg1	model					1139:1143	Korsmeyer-Peppas kinetic model	1114:1143	Korsmeyer-Peppas kinetic model of drug release	1114:1159	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	2	7	theme	Radiation	399:407	arg1	method					409:414	Radiation method	399:414	Radiation method	399:414	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	6	8	theme	drug	1015:1018	arg1	"					1032:1032	anti-cancer drug "gemcitabine"	1003:1032	anti-cancer drug "gemcitabine"	1003:1032	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	6	9	theme	Korsmeyer-Peppas	1114:1129	arg1	model					1139:1143	Korsmeyer-Peppas kinetic model	1114:1143	Korsmeyer-Peppas kinetic model of drug release	1114:1159	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	3	10	theme	13C	563:565	arg1	NMR					567:569	13C NMR	563:569	13C NMR solid state	563:581	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	4	11	theme	antioxidant	686:696	arg1	activity					698:705	antioxidant activity	686:705	antioxidant activity	686:705	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	4	12	theme	biomedical	607:616	arg1	properties					618:627	Some biomedical properties	602:627	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength	602:736	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	8	13	theme	strong	1410:1415	arg1	influence					1417:1425	strong influence	1410:1425	strong influence	1410:1425	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	8	14	theme	delivery	1373:1380	arg1	properties					1382:1391	its mechanical and drug delivery properties	1349:1391	properties	1382:1391	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	3	15	theme	NMR	567:569	arg1	state					577:581	13C NMR solid state	563:581	13C NMR solid state	563:581	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	3	15	theme	NMR	567:569	arg1	Cryo-SEMs					547:555	Cryo-SEMs	547:555	Cryo-SEMs	547:555	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	6	16	theme	gemcitabine	1021:1031	arg1	"					1032:1032	anti-cancer drug "gemcitabine"	1003:1032	anti-cancer drug "gemcitabine"	1003:1032	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	4	17	theme	gel	725:727	arg1	strength					729:736	gel strength	725:736	gel strength	725:736	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	2	18	theme	sterculia	461:469	arg1	gum					471:473	sterculia gum	461:473	sterculia gum	461:473	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	1	19	theme	pharmaceutical	186:199	arg1	applications					201:212	the therapeutic and pharmaceutical applications	166:212	the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration	166:261	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	0	20	theme	biomedical	120:129	arg1	applications					131:142	biomedical applications	120:142	biomedical applications	120:142	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	7	21	from	non-thrombogenic	1193:1208	arg1	nature					1259:1264	nature	1259:1264	nature	1259:1264	The hydrogels were found to be non-thrombogenic, non-haemolytic, mucoadhesive and antioxidant in nature.
29746967	5	22	theme	delivery	857:864	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	8	23	dep	nano-sheets	1291:1301	arg1	GO					1288:1289	GO	1288:1289	GO	1288:1289	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	2	24	theme	graphene	489:496	arg1	oxide					498:502	graphene oxide	489:502	graphene oxide (GO)	489:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	6	25	theme	diffusion	1055:1063	arg1	mechanism					1065:1073	non-Fickian diffusion mechanism	1043:1073	non-Fickian diffusion mechanism	1043:1073	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	4	26	theme	drug	769:772	arg1	studies					783:789	the drug delivery studies	765:789	the drug delivery studies	765:789	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	5	27	theme	slow	895:898	arg1	delivery					900:907	the slow delivery	891:907	the slow delivery of gemcitabine, an anti-cancer drug,	891:944	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	5	28	theme	gum	842:844	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	3	29	theme	swelling	584:591	arg1	studies					593:599	swelling studies	584:599	swelling studies	584:599	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	3	29	theme	swelling	584:591	arg1	Cryo-SEMs					547:555	Cryo-SEMs	547:555	Cryo-SEMs	547:555	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	0	30	theme	network	49:55	arg1	formation					57:65	network formation	49:65	network formation	49:65	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	1	31	theme	sterculia	217:225	arg1	polysaccharide					231:244	sterculia gum polysaccharide	217:244	sterculia gum polysaccharide	217:244	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	2	32	theme	carbopol	476:483	arg1	network					450:456	sterile network	442:456	sterile network of sterculia gum, carbopol and graphene oxide (GO)	442:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	6	33	theme	release	984:990	arg1	profile					992:998	The release profile	980:998	The release profile of anti-cancer drug "gemcitabine"	980:1032	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	2	34	used	used	420:423	arg2	method					409:414	Radiation method	399:414	Radiation method	399:414	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	3	35	theme	solid	571:575	arg1	state					577:581	13C NMR solid state	563:581	13C NMR solid state	563:581	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	3	35	theme	solid	571:575	arg1	Cryo-SEMs					547:555	Cryo-SEMs	547:555	Cryo-SEMs	547:555	These polymers were characterized by Cryo-SEMs, AFM, 13C NMR solid state, swelling studies.
29746967	2	36	theme	gum	471:473	arg1	network					450:456	sterile network	442:456	sterile network of sterculia gum, carbopol and graphene oxide (GO)	442:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	0	37	theme	sterculia	16:24	arg1	polysaccharide					30:43	sterculia gum polysaccharide	16:43	sterculia gum polysaccharide	16:43	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	4	38	theme	delivery	774:781	arg1	studies					783:789	the drug delivery studies	765:789	the drug delivery studies	765:789	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	1	39	theme	polysaccharide	231:244	arg1	applications					201:212	the therapeutic and pharmaceutical applications	166:212	the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration	166:261	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	5	40	theme	present	805:811	arg1	work					813:816	the present work	801:816	the present work	801:816	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	8	41	theme	hydrogel	1320:1327	arg1	matrix					1329:1334	the composite hydrogel matrix	1306:1334	the composite hydrogel matrix	1306:1334	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	8	42	theme	hydrogels	1471:1479	arg1	density					1442:1448	network density	1434:1448	network density	1434:1448	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	8	42	theme	hydrogels	1471:1479	arg1	size					1459:1462	mesh size	1454:1462	mesh size	1454:1462	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	8	43	theme	network	1434:1440	arg1	density					1442:1448	network density	1434:1448	network density	1434:1448	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	8	44	theme	composite	1310:1318	arg1	matrix					1329:1334	the composite hydrogel matrix	1306:1334	the composite hydrogel matrix	1306:1334	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	2	45	theme	sterile	442:448	arg1	network					450:456	sterile network	442:456	sterile network of sterculia gum, carbopol and graphene oxide (GO)	442:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	0	46	theme	polysaccharide	30:43	arg1	Modification					0:11	Modification	0:11	Modification of sterculia gum polysaccharide via network formation by radiation	0:78	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	0	47	theme	gum	26:28	arg1	polysaccharide					30:43	sterculia gum polysaccharide	16:43	sterculia gum polysaccharide	16:43	Modification of sterculia gum polysaccharide via network formation by radiation induced crosslinking polymerization for biomedical applications.
29746967	2	48	theme	network	450:456	arg1	formation					429:437	formation	429:437	formation of sterile network of sterculia gum, carbopol and graphene oxide (GO)	429:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	8	49	theme	nano-sheets	1291:1301	arg1	Incorporation					1267:1279	Incorporation	1267:1279	Incorporation of the GO nano-sheets in the composite hydrogel matrix	1267:1334	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	5	50	theme	sterile	819:825	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	8	51	dep	density	1442:1448	arg1	the					1430:1432	the	1430:1432	the	1430:1432	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	5	52	theme	anti-cancer	928:938	arg1	drug					940:943	an anti-cancer drug	925:943	an anti-cancer drug	925:943	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	5	52	theme	anti-cancer	928:938	arg1	gemcitabine					912:922	gemcitabine	912:922	gemcitabine	912:922	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	2	53	theme	oxide	498:502	arg1	network					450:456	sterile network	442:456	sterile network of sterculia gum, carbopol and graphene oxide (GO)	442:507	Radiation method was used for formation of sterile network of sterculia gum, carbopol and graphene oxide (GO).
29746967	5	54	theme	based	846:850	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	6	55	theme	release	1153:1159	arg1	model					1139:1143	Korsmeyer-Peppas kinetic model	1114:1143	Korsmeyer-Peppas kinetic model of drug release	1114:1159	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	5	56	dep	SCOPE	792:796	arg1	work					813:816	the present work	801:816	the present work	801:816	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	6	57	dep	CONCLUSIONS	968:978	arg1	followed					1034:1041	followed	1034:1041	followed non-Fickian diffusion mechanism and release profile	1034:1093	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	8	58	from	Incorporation	1267:1279	arg1	matrix					1329:1334	the composite hydrogel matrix	1306:1334	the composite hydrogel matrix	1306:1334	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	6	59	theme	drug	1148:1151	arg1	release					1153:1159	drug release	1148:1159	drug release	1148:1159	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	6	60	theme	non-Fickian	1043:1053	arg1	mechanism					1065:1073	non-Fickian diffusion mechanism	1043:1073	non-Fickian diffusion mechanism	1043:1073	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	7	61	from	nature	1259:1264	arg1	non-thrombogenic					1193:1208	non-thrombogenic	1193:1208	non-thrombogenic	1193:1208	The hydrogels were found to be non-thrombogenic, non-haemolytic, mucoadhesive and antioxidant in nature.
29746967	7	61	from	nature	1259:1264	arg1	hydrogels					1166:1174	The hydrogels	1162:1174	The hydrogels	1162:1174	The hydrogels were found to be non-thrombogenic, non-haemolytic, mucoadhesive and antioxidant in nature.
29746967	8	62	theme	mesh	1454:1457	arg1	size					1459:1462	mesh size	1454:1462	mesh size	1454:1462	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	5	63	theme	gemcitabine	912:922	arg1	delivery					900:907	the slow delivery	891:907	the slow delivery of gemcitabine, an anti-cancer drug,	891:944	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	8	64	theme	drug	1368:1371	arg1	properties					1382:1391	its mechanical and drug delivery properties	1349:1391	properties	1382:1391	Incorporation of the GO nano-sheets in the composite hydrogel matrix has improved its mechanical and drug delivery properties and also exerted strong influence on the network density and mesh size of the hydrogels.
29746967	4	65	theme	haemolytic	664:673	arg1	potential					675:683	haemolytic potential	664:683	haemolytic potential	664:683	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
29746967	1	66	theme	gum	227:229	arg1	polysaccharide					231:244	sterculia gum polysaccharide	217:244	sterculia gum polysaccharide	217:244	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	6	67	theme	"	1032:1032	arg1	profile					992:998	The release profile	980:998	The release profile of anti-cancer drug "gemcitabine"	980:1032	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	6	68	theme	anti-cancer	1003:1013	arg1	"					1032:1032	anti-cancer drug "gemcitabine"	1003:1032	anti-cancer drug "gemcitabine"	1003:1032	CONCLUSIONS The release profile of anti-cancer drug "gemcitabine" followed non-Fickian diffusion mechanism and release profile was best fitted in Korsmeyer-Peppas kinetic model of drug release.
29746967	1	69	theme	enhanced	365:372	arg1	applications					385:396	enhanced biomedical applications	365:396	enhanced biomedical applications	365:396	AIMS Keeping in view the therapeutic and pharmaceutical applications of sterculia gum polysaccharide in consideration, its modification has been carried out through grafting and crosslinking to develop the hydrogels for enhanced biomedical applications.
29746967	5	70	theme	drug	852:855	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	5	71	theme	polysaccharide	827:840	arg1	system					866:871	sterile polysaccharide gum based drug delivery system	819:871	sterile polysaccharide gum based drug delivery system	819:871	SCOPE In the present work, sterile polysaccharide gum based drug delivery system was developed for the slow delivery of gemcitabine, an anti-cancer drug, to overcome its side.
29746967	4	72	theme	hydrogels	632:640	arg1	properties					618:627	Some biomedical properties	602:627	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength	602:736	Some biomedical properties of hydrogels like thrombogenicity, haemolytic potential, antioxidant activity, mucoadhesion and gel strength were determined along with the drug delivery studies.
30554009	0	0	theme	xanthan-acacia	75:88	arg1	super-adsorbent					97:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	2	1	theme	composite	492:500	arg1	design					502:507	response surface methodology-central composite design	455:507	response surface methodology-central composite design (RSM-CCD)	455:517	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	2	1	theme	composite	492:500	arg1	RSM-CCD					510:516	RSM-CCD	510:516	RSM-CCD	510:516	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	8	2	theme	industrial	1424:1433	arg1	purpose					1435:1441	industrial purpose	1424:1441	industrial purpose	1424:1441	Semi-IPN could be recycled up to eight consecutive cycles and hence, can be utilized for industrial purpose for removal of dyes.
30554009	1	3	theme	xanthan	171:177	arg1	Blending					159:166	Blending	159:166	Blending of xanthan and acacia	159:188	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	1	4	theme	semi-interpenetrating	247:267	arg1	network					269:275	semi-interpenetrating network	247:275	semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm))	247:417	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	1	4	theme	semi-interpenetrating	247:267	arg1	semi-IPN					278:285	semi-IPN	278:285	semi-IPN	278:285	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	0	5	theme	performance	63:73	arg1	super-adsorbent					97:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	8	6	theme	dyes	1458:1461	arg1	removal					1447:1453	removal	1447:1453	removal of dyes	1447:1461	Semi-IPN could be recycled up to eight consecutive cycles and hence, can be utilized for industrial purpose for removal of dyes.
30554009	5	7	theme	dye	876:878	arg1	capacity					888:895	maximum dye removal capacity	868:895	maximum dye removal capacity of 97.58% and 95.42%, respectively	868:930	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	8	theme	removal	880:886	arg1	capacity					888:895	maximum dye removal capacity	868:895	maximum dye removal capacity of 97.58% and 95.42%, respectively	868:930	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	1	9	theme	network	269:275	arg1	synthesis					234:242	the synthesis	230:242	the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm))	230:417	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	5	10	from	removal	786:792	arg1	blend					756:760	The blend	752:760	The blend	752:760	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	10	from	removal	786:792	arg1	effective					773:781	effective	773:781	effective	773:781	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	11	dep	cationic	802:809	arg1	violet					820:825	crystal violet	812:825	crystal violet	812:825	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	6	12	theme	order	1210:1214	arg1	kinetics					1216:1223	Second order kinetics	1203:1223	Second order kinetics	1203:1223	Second order kinetics was followed in case of both dyes.
30554009	0	13	theme	hybrid	90:95	arg1	super-adsorbent					97:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	3	14	theme	semi-IPN	551:558	arg1	swelling					539:546	Maximum percentage swelling	520:546	Maximum percentage swelling of semi-IPN obtained	520:567	Maximum percentage swelling of semi-IPN obtained was 496.57%.
30554009	5	15	theme	%	905:905	arg1	capacity					888:895	maximum dye removal capacity	868:895	maximum dye removal capacity of 97.58% and 95.42%, respectively	868:930	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	16	theme	anionic	832:838	arg1	dyes					840:843	both cationic (crystal violet) and anionic dyes	797:843	both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters	797:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	16	theme	anionic	832:838	arg1	yellow					852:857	eosin yellow	846:857	eosin yellow	846:857	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	1	17	theme	xanthan-acacia	349:362	arg1	hybrid					339:344	hybrid	339:344	hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm))	339:417	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	3	18	theme	percentage	528:537	arg1	swelling					539:546	Maximum percentage swelling	520:546	Maximum percentage swelling of semi-IPN obtained	520:567	Maximum percentage swelling of semi-IPN obtained was 496.57%.
30554009	5	19	from	dose	976:979	arg1	blend					756:760	The blend	752:760	The blend	752:760	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	19	from	dose	976:979	arg1	effective					773:781	effective	773:781	effective	773:781	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	1	20	theme	acacia	183:188	arg1	Blending					159:166	Blending	159:166	Blending of xanthan and acacia	159:188	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	5	21	theme	dyes	1076:1079	arg1	mechanism					1054:1062	16 h. Adsorption mechanism	1037:1062	16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria	1037:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	22	theme	langmuir	1173:1180	arg1	adsorption					1182:1191	mono layer langmuir adsorption	1162:1191	mono layer langmuir adsorption criteria	1162:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	8	23	theme	consecutive	1374:1384	arg1	cycles					1386:1391	up to eight consecutive cycles	1362:1391	up to eight consecutive cycles	1362:1391	Semi-IPN could be recycled up to eight consecutive cycles and hence, can be utilized for industrial purpose for removal of dyes.
30554009	5	24	theme	eosin	846:850	arg1	dyes					840:843	both cationic (crystal violet) and anionic dyes	797:843	both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters	797:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	24	theme	eosin	846:850	arg1	yellow					852:857	eosin yellow	846:857	eosin yellow	846:857	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	25	theme	cationic	802:809	arg1	dyes					840:843	both cationic (crystal violet) and anionic dyes	797:843	both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters	797:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	25	theme	cationic	802:809	arg1	yellow					852:857	eosin yellow	846:857	eosin yellow	846:857	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	2	26	theme	methodology-central	472:490	arg1	design					502:507	response surface methodology-central composite design	455:507	response surface methodology-central composite design (RSM-CCD)	455:517	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	2	26	theme	methodology-central	472:490	arg1	RSM-CCD					510:516	RSM-CCD	510:516	RSM-CCD	510:516	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	0	27	theme	Efficient	0:8	arg1	capture					10:16	Efficient capture	0:16	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent	0:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	4	28	theme	significant	728:738	arg1	concentrations					586:599	The concentrations	582:599	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively	582:709	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	4	28	theme	significant	728:738	arg1	parameters					740:749	significant parameters	728:749	significant parameters	728:749	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	1	29	theme	unique	198:203	arg1	hybrid					205:210	a unique hybrid	196:210	a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm))	196:417	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	0	30	theme	eosin	21:25	arg1	yellow					27:32	eosin yellow	21:32	eosin yellow	21:32	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	7	31	theme	adsorption	1323:1332	arg1	nature					1313:1318	the exothermic nature	1298:1318	the exothermic nature of adsorption	1298:1332	Thermodynamic studies gave idea about the exothermic nature of adsorption.
30554009	0	32	theme	surface	138:144	arg1	methodology					146:156	response surface methodology	129:156	response surface methodology	129:156	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	6	33	theme	Second	1203:1208	arg1	kinetics					1216:1223	Second order kinetics	1203:1223	Second order kinetics	1203:1223	Second order kinetics was followed in case of both dyes.
30554009	5	34	theme	%	916:916	arg1	capacity					888:895	maximum dye removal capacity	868:895	maximum dye removal capacity of 97.58% and 95.42%, respectively	868:930	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	35	from	solution	994:1001	arg1	blend					756:760	The blend	752:760	The blend	752:760	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	35	from	solution	994:1001	arg1	effective					773:781	effective	773:781	effective	773:781	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	4	36	theme	ammonium	632:639	arg1	persulphate					641:651	ammonium persulphate	632:651	ammonium persulphate	632:651	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	5	37	theme	mono	1162:1165	arg1	adsorption					1182:1191	mono layer langmuir adsorption	1162:1191	mono layer langmuir adsorption criteria	1162:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	0	38	theme	response	129:136	arg1	methodology					146:156	response surface methodology	129:156	response surface methodology	129:156	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	5	39	theme	16 h.	1037:1041	arg1	mechanism					1054:1062	16 h. Adsorption mechanism	1037:1062	16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria	1037:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	8	40	dep	eight	1368:1372	arg1	to					1365:1366	to	1365:1366	to	1365:1366	Semi-IPN could be recycled up to eight consecutive cycles and hence, can be utilized for industrial purpose for removal of dyes.
30554009	5	41	theme	crystal	812:818	arg1	violet					820:825	crystal violet	812:825	crystal violet	812:825	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	2	42	theme	surface	464:470	arg1	design					502:507	response surface methodology-central composite design	455:507	response surface methodology-central composite design (RSM-CCD)	455:517	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	2	42	theme	surface	464:470	arg1	RSM-CCD					510:516	RSM-CCD	510:516	RSM-CCD	510:516	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	1	43	theme	poly	297:300	arg1	chains					314:319	poly(acrylamide) chains	297:319	poly(acrylamide) chains	297:319	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	8	44	used	utilized	1411:1418	arg2	Semi-IPN					1335:1342	Semi-IPN	1335:1342	Semi-IPN	1335:1342	Semi-IPN could be recycled up to eight consecutive cycles and hence, can be utilized for industrial purpose for removal of dyes.
30554009	5	45	theme	Adsorption	1043:1052	arg1	mechanism					1054:1062	16 h. Adsorption mechanism	1037:1062	16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria	1037:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	1	46	used	used	221:224	arg2	hybrid					205:210	a unique hybrid	196:210	a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm))	196:417	Blending of xanthan and acacia gives a unique hybrid that was used for the synthesis of semi-interpenetrating network (semi-IPN) in which poly(acrylamide) chains were grafted onto hybrid of xanthan-acacia followed by their cross-linking (Xan-Aca-cl-poly(AAm)).
30554009	7	47	theme	exothermic	1302:1311	arg1	nature					1313:1318	the exothermic nature	1298:1318	the exothermic nature of adsorption	1298:1332	Thermodynamic studies gave idea about the exothermic nature of adsorption.
30554009	5	48	theme	adsorption	1182:1191	arg1	criteria					1193:1200	mono layer langmuir adsorption criteria	1162:1200	mono layer langmuir adsorption criteria	1162:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	2	49	theme	response	455:462	arg1	design					502:507	response surface methodology-central composite design	455:507	response surface methodology-central composite design (RSM-CCD)	455:517	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	2	49	theme	response	455:462	arg1	RSM-CCD					510:516	RSM-CCD	510:516	RSM-CCD	510:516	Optimization was carried out under response surface methodology-central composite design (RSM-CCD).
30554009	5	50	dep	dyes	1076:1079	arg1	both					1067:1070	both	1067:1070	both	1067:1070	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	0	51	theme	yellow	27:32	arg1	capture					10:16	Efficient capture	0:16	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent	0:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	5	52	theme	15 ml	984:988	arg1	solution					994:1001	15 ml dye solution	984:1001	15 ml dye solution of 10 mg L-1 concentration	984:1028	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	53	theme	layer	1167:1171	arg1	adsorption					1182:1191	mono layer langmuir adsorption	1162:1191	mono layer langmuir adsorption criteria	1162:1200	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	4	54	theme	persulphate	641:651	arg1	concentrations					586:599	The concentrations	582:599	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively	582:709	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	4	54	theme	persulphate	641:651	arg1	parameters					740:749	significant parameters	728:749	significant parameters	728:749	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	0	55	theme	violet	46:51	arg1	capture					10:16	Efficient capture	0:16	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent	0:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	5	56	theme	intraparticle	1121:1133	arg1	model					1145:1149	intraparticle diffusion model	1121:1149	intraparticle diffusion model	1121:1149	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	57	theme	optimized	938:946	arg1	parameters					948:957	optimized parameters	938:957	optimized parameters	938:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	58	theme	dye	990:992	arg1	solution					994:1001	15 ml dye solution	984:1001	15 ml dye solution of 10 mg L-1 concentration	984:1028	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	0	59	theme	crystal	38:44	arg1	violet					46:51	crystal violet	38:51	crystal violet	38:51	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	5	60	theme	diffusion	1135:1143	arg1	model					1145:1149	intraparticle diffusion model	1121:1149	intraparticle diffusion model	1121:1149	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	61	from	effective	773:781	arg1	dose					976:979	0.4 g semi-IPN dose	961:979	0.4 g semi-IPN dose	961:979	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	61	from	effective	773:781	arg1	removal					786:792	removal	786:792	removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters	786:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	61	from	effective	773:781	arg1	solution					994:1001	15 ml dye solution	984:1001	15 ml dye solution of 10 mg L-1 concentration	984:1028	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	0	62	with	capture	10:16	arg1	super-adsorbent					97:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	7	63	theme	Thermodynamic	1260:1272	arg1	studies					1274:1280	Thermodynamic studies	1260:1280	Thermodynamic studies	1260:1280	Thermodynamic studies gave idea about the exothermic nature of adsorption.
30554009	5	64	theme	10 mg L-1	1006:1014	arg1	concentration					1016:1028	10 mg L-1 concentration	1006:1028	10 mg L-1 concentration	1006:1028	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	65	theme	concentration	1016:1028	arg1	solution					994:1001	15 ml dye solution	984:1001	15 ml dye solution of 10 mg L-1 concentration	984:1028	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	66	theme	0.4 g	961:965	arg1	dose					976:979	0.4 g semi-IPN dose	961:979	0.4 g semi-IPN dose	961:979	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	67	theme	maximum	868:874	arg1	capacity					888:895	maximum dye removal capacity	868:895	maximum dye removal capacity of 97.58% and 95.42%, respectively	868:930	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	4	68	theme	acrylamide	604:613	arg1	concentrations					586:599	The concentrations	582:599	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively	582:709	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	4	68	theme	acrylamide	604:613	arg1	parameters					740:749	significant parameters	728:749	significant parameters	728:749	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	0	69	theme	high	58:61	arg1	super-adsorbent					97:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	high performance xanthan-acacia hybrid super-adsorbent	58:111	Efficient capture of eosin yellow and crystal violet with high performance xanthan-acacia hybrid super-adsorbent optimized using response surface methodology.
30554009	5	70	theme	dyes	840:843	arg1	removal					786:792	removal	786:792	removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters	786:957	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	5	71	theme	semi-IPN	967:974	arg1	dose					976:979	0.4 g semi-IPN dose	961:979	0.4 g semi-IPN dose	961:979	The blend was highly effective in removal of both cationic (crystal violet) and anionic dyes (eosin yellow) showing maximum dye removal capacity of 97.58% and 95.42%, respectively under optimized parameters - 0.4 g semi-IPN dose in 15 ml dye solution of 10 mg L-1 concentration within 16 h. Adsorption mechanism of both the dyes followed three steps in accordance with intraparticle diffusion model along with mono layer langmuir adsorption criteria.
30554009	4	72	theme	acid	623:626	arg1	concentrations					586:599	The concentrations	582:599	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively	582:709	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	4	72	theme	acid	623:626	arg1	parameters					740:749	significant parameters	728:749	significant parameters	728:749	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30554009	3	73	theme	Maximum	520:526	arg1	swelling					539:546	Maximum percentage swelling	520:546	Maximum percentage swelling of semi-IPN obtained	520:567	Maximum percentage swelling of semi-IPN obtained was 496.57%.
30554009	4	74	theme	citric	616:621	arg1	acid					623:626	citric acid	616:626	citric acid	616:626	The concentrations of acrylamide, citric acid and ammonium persulphate used as monomer, cross-linker and initiator, respectively were found to be significant parameters.
30111709	2	0	theme	kDa	499:501	arg1	content					461:467	21% sulfate content	449:467	21% sulfate content	449:467	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	2	0	theme	kDa	499:501	arg1	weight					485:490	a molecular weight	473:490	a molecular weight of 5.0 kDa	473:501	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	9	1	theme	green	1202:1206	arg1	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	1	theme	green	1202:1206	arg1	food					1250:1253	food	1250:1253	food	1250:1253	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	1	theme	green	1202:1206	arg1	source					1323:1328	a source	1321:1328	a source of bioactive ulvan	1321:1347	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	1	theme	green	1202:1206	arg1	herb					1278:1281	traditional marine herb	1259:1281	traditional marine herb	1259:1281	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	5	2	located	present	901:907	arg2	groups					882:887	The sulfate groups	870:887	The sulfate groups	870:887	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	2	located	present	901:907	arg1	position					920:927	the O-3 position	912:927	the O-3 position of →4)-α-L-Rha(1→	912:945	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	2	located	present	901:907	arg2	present					901:907	present	901:907	present	901:907	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	9	3	theme	bioactive	1333:1341	arg1	ulvan					1343:1347	bioactive ulvan	1333:1347	bioactive ulvan	1333:1347	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	4	dep	food	1250:1253	arg1	a					1248:1248	a	1248:1248	a	1248:1248	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	2	5	with	ratio	431:435	arg1	content					461:467	21% sulfate content	449:467	21% sulfate content	449:467	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	2	5	with	ratio	431:435	arg1	weight					485:490	a molecular weight	473:490	a molecular weight of 5.0 kDa	473:501	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	3	6	theme	magnetic	680:687	arg1	resonance					689:697	nuclear magnetic resonance	672:697	nuclear magnetic resonance (NMR)	672:703	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	6	theme	magnetic	680:687	arg1	NMR					700:702	NMR	700:702	NMR	700:702	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	5	7	from	present	901:907	arg1	position					920:927	the O-3 position	912:927	the O-3 position of →4)-α-L-Rha(1→	912:945	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	7	8	theme	O-2	1051:1053	arg1	position					1055:1062	the O-2 position	1047:1062	the O-2 position of the →4)-α-L-Rha(1→	1047:1084	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	1	9	theme	size-exclusion	261:274	arg1	chromatography					276:289	ion-exchange and size-exclusion chromatography	244:289	chromatography	276:289	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	9	10	theme	Ulothrix	1214:1221	arg1	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	10	theme	Ulothrix	1214:1221	arg1	food					1250:1253	food	1250:1253	food	1250:1253	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	10	theme	Ulothrix	1214:1221	arg1	source					1323:1328	a source	1321:1328	a source of bioactive ulvan	1321:1347	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	10	theme	Ulothrix	1214:1221	arg1	herb					1278:1281	traditional marine herb	1259:1281	traditional marine herb	1259:1281	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	7	11	theme	1→	1034:1035	arg1	branch					1037:1042	→4)-α-L-Rha(1→ branch	1022:1042	→4)-α-L-Rha(1→ branch	1022:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	3	12	theme	methylation	564:574	arg1	analyses					576:583	desulfation and methylation analyses	548:583	desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR)	548:703	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	0	13	theme	Green	77:81	arg1	Alga					83:86	the Green Alga	73:86	the Green Alga Ulothrix flacca	73:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	0	14	dep	Ulothrix	88:95	arg1	flacca					97:102	flacca	97:102	flacca	97:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	2	15	theme	characteristics	308:322	arg1	analyses					324:331	Composition and characteristics analyses	292:331	Composition and characteristics analyses	292:331	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	7	16	theme	1→	1083:1084	arg1	position					1055:1062	the O-2 position	1047:1062	the O-2 position of the →4)-α-L-Rha(1→	1047:1084	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	4	17	theme	4-linked-β-D-glucouronoc	830:853	arg1	residues					860:867	4-linked-β-D-glucouronoc acid residues	830:867	4-linked-β-D-glucouronoc acid residues	830:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	2	18	from	glucuronorhamnan	366:381	arg1	ratio					431:435	a ratio	429:435	a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa	429:501	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	3	19	theme	desulfation	548:558	arg1	analyses					576:583	desulfation and methylation analyses	548:583	desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR)	548:703	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	0	20	theme	Ulothrix	88:95	arg1	Alga					83:86	the Green Alga	73:86	the Green Alga Ulothrix flacca	73:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	9	21	from	food	1250:1253	arg1	China					1286:1290	China	1286:1290	China	1286:1290	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	5	22	from	position	920:927	arg1	present					901:907	present	901:907	present	901:907	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	22	from	position	920:927	arg1	groups					882:887	The sulfate groups	870:887	The sulfate groups	870:887	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	2	23	theme	Composition	292:302	arg1	analyses					324:331	Composition and characteristics analyses	292:331	Composition and characteristics analyses	292:331	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	7	24	contain	had	1000:1002	arg2	branch					1037:1042	→4)-α-L-Rha(1→ branch	1022:1042	→4)-α-L-Rha(1→ branch	1022:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	24	contain	had	1000:1002	arg2	amount					1012:1017	a small amount	1004:1017	a small amount of →4)-α-L-Rha(1→ branch	1004:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	24	contain	had	1000:1002	arg1	UP2-1					989:993	UP2-1	989:993	UP2-1	989:993	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	25	theme	-α-L-Rha	1074:1081	arg1	1→					1083:1084	the →4)-α-L-Rha(1→	1067:1084	the →4)-α-L-Rha(1→	1067:1084	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	5	26	attach	present	901:907	arg2	groups					882:887	The sulfate groups	870:887	The sulfate groups	870:887	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	26	attach	present	901:907	arg1	position					920:927	the O-3 position	912:927	the O-3 position of →4)-α-L-Rha(1→	912:945	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	26	attach	present	901:907	arg2	present					901:907	present	901:907	present	901:907	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	3	27	theme	infrared	599:606	arg1	IR					618:619	IR	618:619	IR	618:619	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	27	theme	infrared	599:606	arg1	spectrum					608:615	infrared spectrum	599:615	infrared spectrum (IR)	599:620	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	5	28	theme	sulfate	874:880	arg1	present					901:907	present	901:907	present	901:907	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	5	28	theme	sulfate	874:880	arg1	groups					882:887	The sulfate groups	870:887	The sulfate groups	870:887	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	4	29	theme	acid	855:858	arg1	residues					860:867	4-linked-β-D-glucouronoc acid residues	830:867	4-linked-β-D-glucouronoc acid residues	830:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	7	30	theme	-α-L-Rha	1025:1032	arg1	branch					1037:1042	→4)-α-L-Rha(1→ branch	1022:1042	→4)-α-L-Rha(1→ branch	1022:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	3	31	theme	nuclear	672:678	arg1	resonance					689:697	nuclear magnetic resonance	672:697	nuclear magnetic resonance (NMR)	672:703	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	31	theme	nuclear	672:678	arg1	NMR					700:702	NMR	700:702	NMR	700:702	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	2	32	theme	sulfate	453:459	arg1	content					461:467	21% sulfate content	449:467	21% sulfate content	449:467	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	9	33	dep	Ulothrix	1214:1221	arg1	flacca					1223:1228	flacca	1223:1228	flacca	1223:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	7	34	theme	branch	1037:1042	arg1	branch					1037:1042	→4)-α-L-Rha(1→ branch	1022:1042	→4)-α-L-Rha(1→ branch	1022:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	34	theme	branch	1037:1042	arg1	amount					1012:1017	a small amount	1004:1017	a small amount of →4)-α-L-Rha(1→ branch	1004:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	4	35	theme	ulvan	750:754	arg1	UP2-1					730:734	UP2-1	730:734	UP2-1	730:734	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	4	35	theme	ulvan	750:754	arg1	type					742:745	a type	740:745	a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues	740:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	2	36	theme	21	449:450	arg1	%					451:451	%	451:451	%	451:451	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	2	37	theme	%	451:451	arg1	content					461:467	21% sulfate content	449:467	21% sulfate content	449:467	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	4	38	theme	4-linked-α-L-rhamnose	778:798	arg1	residues					800:807	alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues	768:867	alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues	768:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	2	39	theme	molecular	475:483	arg1	weight					485:490	a molecular weight	473:490	a molecular weight of 5.0 kDa	473:501	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	2	40	theme	glucuronic	410:419	arg1	acid					421:424	glucuronic acid	410:424	glucuronic acid	410:424	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	5	41	theme	O-3	916:918	arg1	position					920:927	the O-3 position	912:927	the O-3 position of →4)-α-L-Rha(1→	912:945	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	1	42	theme	marine	204:209	arg1	algae					217:221	the marine green algae	200:221	the marine green algae	200:221	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	4	43	dep	residues	800:807	arg1	residues					860:867	4-linked-β-D-glucouronoc acid residues	830:867	4-linked-β-D-glucouronoc acid residues	830:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	4	43	dep	residues	800:807	arg1	-α-L-Rha					813:820	(→4)-α-L-Rha(1→)	809:824	(→4)-α-L-Rha(1→)	809:824	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	2	44	theme	2:1	440:442	arg1	ratio					431:435	a ratio	429:435	a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa	429:501	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	8	45	theme	significant	1103:1113	arg1	anticoagulant					1115:1127	significant anticoagulant and immunomodulating activity	1103:1157	anticoagulant	1115:1127	UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro.
30111709	7	46	theme	small	1006:1010	arg1	branch					1037:1042	→4)-α-L-Rha(1→ branch	1022:1042	→4)-α-L-Rha(1→ branch	1022:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	46	theme	small	1006:1010	arg1	amount					1012:1017	a small amount	1004:1017	a small amount of →4)-α-L-Rha(1→ branch	1004:1042	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	7	47	theme	→4	1071:1072	arg1	1→					1083:1084	the →4)-α-L-Rha(1→	1067:1084	the →4)-α-L-Rha(1→	1067:1084	UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→.
30111709	1	48	theme	green	211:215	arg1	algae					217:221	the marine green algae	200:221	the marine green algae	200:221	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	0	49	theme	Molecular	45:53	arg1	Weight					55:60	a Low Molecular Weight	39:60	a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca	39:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	9	50	theme	traditional	1259:1269	arg1	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	50	theme	traditional	1259:1269	arg1	herb					1278:1281	traditional marine herb	1259:1281	traditional marine herb	1259:1281	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	51	from	herb	1278:1281	arg1	China					1286:1290	China	1286:1290	China	1286:1290	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	0	52	theme	Low	41:43	arg1	Weight					55:60	a Low Molecular Weight	39:60	a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca	39:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	9	53	theme	marine	1271:1276	arg1	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	53	theme	marine	1271:1276	arg1	herb					1278:1281	traditional marine herb	1259:1281	traditional marine herb	1259:1281	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	3	54	theme	Structural	504:513	arg1	properties					515:524	Structural properties	504:524	Structural properties	504:524	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	55	theme	chromatography-mass	627:645	arg1	GC-MS					661:665	GC-MS	661:665	GC-MS	661:665	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	55	theme	chromatography-mass	627:645	arg1	spectrometer					647:658	gas chromatography-mass spectrometer	623:658	gas chromatography-mass spectrometer (GC-MS)	623:666	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	1	56	theme	water-soluble	107:119	arg1	polysaccharide					142:155	A water-soluble low molecular⁻weight polysaccharide	105:155	A water-soluble low molecular⁻weight polysaccharide named UP2-1	105:167	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	5	57	theme	-α-L-Rha	935:942	arg1	position					920:927	the O-3 position	912:927	the O-3 position of →4)-α-L-Rha(1→	912:945	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	4	58	theme	alternate	768:776	arg1	residues					800:807	alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues	768:867	alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues	768:867	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	0	59	from	Alga	83:86	arg1	Ulvan					62:66	Ulvan	62:66	Ulvan	62:66	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	5	60	dep	-α-L-Rha	935:942	arg1	→4					932:933	→4	932:933	→4	932:933	The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→.
30111709	1	61	theme	low	121:123	arg1	polysaccharide					142:155	A water-soluble low molecular⁻weight polysaccharide	105:155	A water-soluble low molecular⁻weight polysaccharide named UP2-1	105:167	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	8	62	theme	immunomodulating	1133:1148	arg1	activity					1150:1157	significant anticoagulant and immunomodulating activity	1103:1157	activity	1150:1157	UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro.
30111709	2	63	theme	sulfated	357:364	arg1	glucuronorhamnan					366:381	a sulfated glucuronorhamnan	355:381	a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid	355:424	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	2	63	theme	sulfated	357:364	arg1	UP2-1					345:349	UP2-1	345:349	UP2-1	345:349	Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa.
30111709	0	64	theme	Ulvan	62:66	arg1	Weight					55:60	a Low Molecular Weight	39:60	a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca	39:102	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	4	65	dep	-α-L-Rha	813:820	arg1	→4					810:811	→4	810:811	→4	810:811	The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues.
30111709	3	66	theme	gas	623:625	arg1	GC-MS					661:665	GC-MS	661:665	GC-MS	661:665	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	3	66	theme	gas	623:625	arg1	spectrometer					647:658	gas chromatography-mass spectrometer	623:658	gas chromatography-mass spectrometer (GC-MS)	623:666	Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR).
30111709	9	67	theme	ulvan	1343:1347	arg1	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	67	theme	ulvan	1343:1347	arg1	source					1323:1328	a source	1321:1328	a source of bioactive ulvan	1321:1347	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	1	68	theme	molecular⁻weight	125:140	arg1	polysaccharide					142:155	A water-soluble low molecular⁻weight polysaccharide	105:155	A water-soluble low molecular⁻weight polysaccharide named UP2-1	105:167	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30111709	0	69	theme	Weight	55:60	arg1	Bioactivity					14:24	Bioactivity	14:24	Bioactivity	14:24	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	0	69	theme	Weight	55:60	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	0	70	dep	Structure	0:8	arg1	Screening					26:34	Screening	26:34	Screening	26:34	Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca.
30111709	9	71	used	used	1240:1243	arg2	herb					1278:1281	traditional marine herb	1259:1281	traditional marine herb	1259:1281	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	71	used	used	1240:1243	arg2	food					1250:1253	food	1250:1253	food	1250:1253	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	71	used	used	1240:1243	arg2	source					1323:1328	a source	1321:1328	a source of bioactive ulvan	1321:1347	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	9	71	used	used	1240:1243	arg2	algae					1208:1212	the green algae	1198:1212	the green algae Ulothrix flacca	1198:1228	This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.
30111709	1	72	theme	ion-exchange	244:255	arg1	chromatography					276:289	ion-exchange and size-exclusion chromatography	244:289	chromatography	276:289	A water-soluble low molecular⁻weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrixflacca using ion-exchange and size-exclusion chromatography.
30277587	9	0	theme	CD	1983:1984	arg1	model					1992:1996	the CD mouse model	1979:1996	the CD mouse model	1979:1996	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	1	1	from	content	308:314	arg1	mice					373:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	2	theme	similar	422:428	arg1	characteristics					430:444	similar characteristics	422:444	similar characteristics	422:444	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	3	theme	mix	266:268	arg1	MF					276:277	MF	276:277	MF	276:277	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	3	theme	mix	266:268	arg1	diet					270:273	a specific multifiber mix diet	244:273	a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	244:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	4	theme	signal	924:929	arg1	transducers					931:941	signal transducers	924:941	signal transducers	924:941	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	5	theme	Proteobacteria	1667:1680	arg1	phylum					1682:1687	the Proteobacteria phylum	1663:1687	the Proteobacteria phylum	1663:1687	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	5	6	theme	cells	1321:1325	arg1	frequency					1299:1307	decreased lamina propria frequency	1274:1307	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	7	theme	decreased	1274:1282	arg1	propria					1291:1297	decreased lamina propria	1274:1297	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	8	theme	propria	1291:1297	arg1	frequency					1299:1307	decreased lamina propria frequency	1274:1307	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	9	9	contain	has	2018:2020	arg1	MF					2015:2016	MF	2015:2016	MF	2015:2016	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	9	9	contain	has	2018:2020	arg2	strategy					2049:2056	a therapeutic strategy	2035:2056	a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD	2035:2128	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	9	9	contain	has	2018:2020	arg2	potential					2022:2030	potential	2022:2030	potential	2022:2030	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	1	10	theme	IL-10-/-	362:369	arg1	mice					373:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	0	11	theme	Macrophage	138:147	arg1	Polarization					149:160	M2 Macrophage Polarization	135:160	M2 Macrophage Polarization	135:160	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	1	12	theme	therapeutic	219:229	arg1	mechanism					231:239	the therapeutic mechanism	215:239	the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	215:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	2	13	theme	MF	560:561	arg1	diet					563:566	MF diet	560:566	MF diet for 4 weeks	560:578	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	4	14	theme	corresponding	1056:1068	arg1	metabolites					1070:1080	the corresponding metabolites	1052:1080	the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1052:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	14	theme	corresponding	1056:1068	arg1	acids					1101:1105	short-chain fatty acids	1083:1105	short-chain fatty acids	1083:1105	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	9	15	theme	mouse	1986:1990	arg1	model					1992:1996	the CD mouse model	1979:1996	the CD mouse model	1979:1996	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	3	16	theme	inflammatory	715:726	arg1	cytokines					728:736	inflammatory cytokines	715:736	inflammatory cytokines	715:736	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	16	theme	inflammatory	715:726	arg1	[TNF-α					778:783	tumor necrosis factor-α [TNF-α	754:783	tumor necrosis factor-α [TNF-α	754:783	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	7	17	theme	Foxp3+	1757:1762	arg1	Tregs					1764:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	1	18	theme	spontaneous	383:393	arg1	colitis					403:409	spontaneous chronic colitis	383:409	spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	383:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	19	theme	inflammatory	804:815	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	19	theme	inflammatory	804:815	arg1	[MIP					825:828	macrophage inflammatory protein [MIP]-2	793:831	macrophage inflammatory protein [MIP]-2	793:831	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	20	from	decrease	1629:1636	arg1	bacteria					1567:1574	bacteria	1567:1574	bacteria belonging to the Firmicutes phylum	1567:1609	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	6	20	from	decrease	1629:1636	arg1	bacteria					1641:1648	bacteria	1641:1648	bacteria belonging to the Proteobacteria phylum	1641:1687	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	6	21	theme	IL-10-/-	1516:1523	arg1	increase					1555:1562	a significant increase	1541:1562	a significant increase in bacteria belonging to the Firmicutes phylum	1541:1609	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	6	21	theme	IL-10-/-	1516:1523	arg1	mice					1525:1528	IL-10-/- mice	1516:1528	IL-10-/- mice	1516:1528	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	6	21	theme	IL-10-/-	1516:1523	arg1	decrease					1629:1636	a significant decrease	1615:1636	a significant decrease in bacteria belonging to the Proteobacteria phylum	1615:1687	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	3	22	theme	necrosis	760:767	arg1	cytokines					728:736	inflammatory cytokines	715:736	inflammatory cytokines	715:736	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	22	theme	necrosis	760:767	arg1	[TNF-α					778:783	tumor necrosis factor-α [TNF-α	754:783	tumor necrosis factor-α [TNF-α	754:783	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	MIP-1α					882:887	MIP-1α	882:887	MIP-1α	882:887	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	composition					606:616	the composition	602:616	the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1]	602:875	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	arginase					902:909	arginase 1	902:911	arginase 1 (Arg1)	902:918	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	transducers					931:941	signal transducers	924:941	signal transducers	924:941	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	Severity					581:588	Severity	581:588	Severity of colitis	581:599	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	23	theme	colitis	593:599	arg1	Arg1					914:917	Arg1	914:917	Arg1	914:917	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	24	from	increase	1434:1441	arg1	diversity					1460:1468	gut microbial diversity	1446:1468	gut microbial diversity	1446:1468	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	3	25	dep	microbiota	631:640	arg1	[MCP-1					869:874	[MCP-1	869:874	[MCP-1	869:874	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	5	26	theme	inflammatory	1352:1363	arg1	MIP-2					1404:1408	MIP-2	1404:1408	MIP-2	1404:1408	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	26	theme	inflammatory	1352:1363	arg1	MCP-1					1411:1415	MCP-1	1411:1415	MCP-1	1411:1415	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	26	theme	inflammatory	1352:1363	arg1	TNF-α					1391:1395	TNF-α	1391:1395	TNF-α	1391:1395	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	26	theme	inflammatory	1352:1363	arg1	IL-6					1398:1401	IL-6	1398:1401	IL-6	1398:1401	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	26	theme	inflammatory	1352:1363	arg1	MIP-1α					1422:1427	MIP-1α	1422:1427	MIP-1α	1422:1427	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	26	theme	inflammatory	1352:1363	arg1	cytokines					1365:1373	inflammatory cytokines	1352:1373	inflammatory cytokines	1352:1373	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	0	27	theme	CD4+	89:92	arg1	Differentiation					94:108	CD4+ Differentiation	89:108	CD4+ Differentiation	89:108	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	1	28	theme	healthy	321:327	arg1	diet					329:332	a healthy diet	319:332	a healthy diet	319:332	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	2	29	theme	IL-10-/-	511:518	arg1	mice					520:523	Sixteen-week-old IL-10-/- mice	494:523	Sixteen-week-old IL-10-/- mice	494:523	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	4	30	theme	MF	1111:1112	arg1	metabolites					1070:1080	the corresponding metabolites	1052:1080	the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1052:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	30	theme	MF	1111:1112	arg1	acids					1101:1105	short-chain fatty acids	1083:1105	short-chain fatty acids	1083:1105	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	2	31	used	used	530:533	arg2	mice					520:523	Sixteen-week-old IL-10-/- mice	494:523	Sixteen-week-old IL-10-/- mice	494:523	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	5	32	theme	MPO	1328:1330	arg1	concentrations					1332:1345	MPO concentrations	1328:1345	MPO concentrations	1328:1345	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	4	33	theme	CD4+	1117:1120	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	33	theme	CD4+	1117:1120	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	9	34	theme	active	2120:2125	arg1	CD					2127:2128	active CD	2120:2128	active CD	2120:2128	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	1	35	theme	knockout	352:359	arg1	mice					373:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	7	36	theme	metabolites	1790:1800	arg1	butyrate					1802:1809	microbial metabolites butyrate	1780:1809	microbial metabolites butyrate	1780:1809	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	3	37	theme	monocyte	834:841	arg1	protein-1					859:867	monocyte chemoattractant protein-1	834:867	monocyte chemoattractant protein-1	834:867	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	37	theme	monocyte	834:841	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	4	38	theme	fatty	1095:1099	arg1	metabolites					1070:1080	the corresponding metabolites	1052:1080	the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1052:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	38	theme	fatty	1095:1099	arg1	acids					1101:1105	short-chain fatty acids	1083:1105	short-chain fatty acids	1083:1105	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	0	39	theme	Dietary	0:6	arg1	Polysaccharides					22:36	Dietary Nondigestible Polysaccharides	0:36	Dietary Nondigestible Polysaccharides	0:36	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	3	40	theme	proteins	985:992	arg1	MIP-1α					882:887	MIP-1α	882:887	MIP-1α	882:887	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	composition					606:616	the composition	602:616	the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1]	602:875	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	arginase					902:909	arginase 1	902:911	arginase 1 (Arg1)	902:918	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	transducers					931:941	signal transducers	924:941	signal transducers	924:941	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	Severity					581:588	Severity	581:588	Severity of colitis	581:599	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	activators					947:956	activators	947:956	activators	947:956	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	40	theme	proteins	985:992	arg1	Arg1					914:917	Arg1	914:917	Arg1	914:917	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	41	theme	microbial	1450:1458	arg1	diversity					1460:1468	gut microbial diversity	1446:1468	gut microbial diversity	1446:1468	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	8	42	theme	MF	1889:1890	arg1	treatment					1892:1900	MF treatment	1889:1900	MF treatment	1889:1900	In addition, Arg1 and STAT6 proteins were also significantly increased after MF treatment.
30277587	7	43	theme	Tregs	1764:1768	arg1	differentiation					1727:1741	the differentiation	1723:1741	the differentiation of CD4+ CD25+ Foxp3+ Tregs	1723:1768	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	7	44	theme	CD25+	1751:1755	arg1	Tregs					1764:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	7	45	theme	MF	1700:1701	arg1	treatment					1703:1711	MF treatment	1700:1711	MF treatment	1700:1711	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	6	46	theme	significant	1617:1627	arg1	decrease					1629:1636	a significant decrease	1615:1636	a significant decrease in bacteria belonging to the Proteobacteria phylum	1615:1687	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	1	47	theme	fiber	302:306	arg1	content					308:314	the fiber content	298:314	the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice	298:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	4	48	theme	Foxp3+	1128:1133	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	48	theme	Foxp3+	1128:1133	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	49	theme	T	1146:1146	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	49	theme	T	1146:1146	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	5	50	theme	MF	1211:1212	arg1	treatment					1214:1222	RESULTS MF treatment	1203:1222	RESULTS MF treatment	1203:1222	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	3	51	theme	tumor	754:758	arg1	cytokines					728:736	inflammatory cytokines	715:736	inflammatory cytokines	715:736	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	51	theme	tumor	754:758	arg1	[TNF-α					778:783	tumor necrosis factor-α [TNF-α	754:783	tumor necrosis factor-α [TNF-α	754:783	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	52	theme	cells	666:670	arg1	expression					643:652	expression	643:652	expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α]	643:784	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	52	theme	cells	666:670	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	1	53	theme	multifiber	255:264	arg1	MF					276:277	MF	276:277	MF	276:277	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	53	theme	multifiber	255:264	arg1	diet					270:273	a specific multifiber mix diet	244:273	a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	244:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	54	theme	human	458:462	arg1	CD					481:482	CD	481:482	CD	481:482	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	54	theme	human	458:462	arg1	disease					472:478	human Crohn's disease	458:478	human Crohn's disease (CD)	458:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	5	55	theme	Th1/Th17	1312:1319	arg1	cells					1321:1325	Th1/Th17 cells	1312:1325	Th1/Th17 cells	1312:1325	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	9	56	dep	CONCLUSIONS	1903:1913	arg1	shed					1929:1932	shed	1929:1932	shed light on the contribution of MF treatment to the CD mouse model	1929:1996	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	9	56	dep	CONCLUSIONS	1903:1913	arg1	suggest					2002:2008	suggest	2002:2008	suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD	2002:2128	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	1	57	theme	diet	270:273	arg1	mechanism					231:239	the therapeutic mechanism	215:239	the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	215:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	58	theme	MPO	690:692	arg1	concentrations					695:708	myeloperoxidase (MPO) concentrations	673:708	myeloperoxidase (MPO) concentrations	673:708	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	59	theme	myeloperoxidase	673:687	arg1	concentrations					695:708	myeloperoxidase (MPO) concentrations	673:708	myeloperoxidase (MPO) concentrations	673:708	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	5	60	theme	lamina	1284:1289	arg1	propria					1291:1297	decreased lamina propria	1274:1297	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	1	61	theme	study	190:194	arg1	aim					178:180	The aim	174:180	The aim of this study	174:194	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	62	theme	microbiota	631:640	arg1	MIP-1α					882:887	MIP-1α	882:887	MIP-1α	882:887	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	62	theme	microbiota	631:640	arg1	composition					606:616	the composition	602:616	the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1]	602:875	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	62	theme	microbiota	631:640	arg1	arginase					902:909	arginase 1	902:911	arginase 1 (Arg1)	902:918	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	62	theme	microbiota	631:640	arg1	transducers					931:941	signal transducers	924:941	signal transducers	924:941	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	62	theme	microbiota	631:640	arg1	Severity					581:588	Severity	581:588	Severity of colitis	581:599	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	62	theme	microbiota	631:640	arg1	Arg1					914:917	Arg1	914:917	Arg1	914:917	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	0	63	theme	M2	135:136	arg1	Polarization					149:160	M2 Macrophage Polarization	135:160	M2 Macrophage Polarization	135:160	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	5	64	dep	cytokines	1365:1373	arg1	MIP-2					1404:1408	MIP-2	1404:1408	MIP-2	1404:1408	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	64	dep	cytokines	1365:1373	arg1	MCP-1					1411:1415	MCP-1	1411:1415	MCP-1	1411:1415	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	64	dep	cytokines	1365:1373	arg1	TNF-α					1391:1395	TNF-α	1391:1395	TNF-α	1391:1395	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	64	dep	cytokines	1365:1373	arg1	IL-6					1398:1401	IL-6	1398:1401	IL-6	1398:1401	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	64	dep	cytokines	1365:1373	arg1	MIP-1α					1422:1427	MIP-1α	1422:1427	MIP-1α	1422:1427	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	5	64	dep	cytokines	1365:1373	arg1	cytokines					1365:1373	inflammatory cytokines	1352:1373	inflammatory cytokines	1352:1373	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	6	65	theme	significant	1543:1553	arg1	increase					1555:1562	a significant increase	1541:1562	a significant increase in bacteria belonging to the Firmicutes phylum	1541:1609	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	3	66	theme	concentrations	695:708	arg1	expression					643:652	expression	643:652	expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α]	643:784	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	66	theme	concentrations	695:708	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	9	67	with	patients	2106:2113	arg1	CD					2127:2128	active CD	2120:2128	active CD	2120:2128	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	9	68	theme	therapeutic	2037:2047	arg1	potential					2022:2030	potential	2022:2030	potential	2022:2030	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	9	68	theme	therapeutic	2037:2047	arg1	strategy					2049:2056	a therapeutic strategy	2035:2056	a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD	2035:2128	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	4	69	theme	short-chain	1083:1093	arg1	metabolites					1070:1080	the corresponding metabolites	1052:1080	the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1052:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	69	theme	short-chain	1083:1093	arg1	acids					1101:1105	short-chain fatty acids	1083:1105	short-chain fatty acids	1083:1105	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	3	70	theme	cytokines	728:736	arg1	expression					643:652	expression	643:652	expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α]	643:784	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	70	theme	cytokines	728:736	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	1	71	theme	chronic	395:401	arg1	colitis					403:409	spontaneous chronic colitis	383:409	spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	383:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	72	theme	protein	817:823	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	72	theme	protein	817:823	arg1	[MIP					825:828	macrophage inflammatory protein [MIP]-2	793:831	macrophage inflammatory protein [MIP]-2	793:831	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	73	theme	factor-α	769:776	arg1	cytokines					728:736	inflammatory cytokines	715:736	inflammatory cytokines	715:736	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	73	theme	factor-α	769:776	arg1	[TNF-α					778:783	tumor necrosis factor-α [TNF-α	754:783	tumor necrosis factor-α [TNF-α	754:783	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	8	74	theme	Arg1	1825:1828	arg1	proteins					1840:1847	Arg1 and STAT6 proteins	1825:1847	Arg1 and STAT6 proteins	1825:1847	In addition, Arg1 and STAT6 proteins were also significantly increased after MF treatment.
30277587	3	75	theme	macrophage	793:802	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	75	theme	macrophage	793:802	arg1	[MIP					825:828	macrophage inflammatory protein [MIP]-2	793:831	macrophage inflammatory protein [MIP]-2	793:831	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	76	from	increase	1555:1562	arg1	bacteria					1567:1574	bacteria	1567:1574	bacteria belonging to the Firmicutes phylum	1567:1609	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	6	76	from	increase	1555:1562	arg1	bacteria					1641:1648	bacteria	1641:1648	bacteria belonging to the Proteobacteria phylum	1641:1687	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	8	77	theme	STAT6	1834:1838	arg1	proteins					1840:1847	Arg1 and STAT6 proteins	1825:1847	Arg1 and STAT6 proteins	1825:1847	In addition, Arg1 and STAT6 proteins were also significantly increased after MF treatment.
30277587	5	78	theme	cytokines	1365:1373	arg1	frequency					1299:1307	decreased lamina propria frequency	1274:1307	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	1	79	theme	diet	329:332	arg1	content					308:314	the fiber content	298:314	the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice	298:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	3	80	theme	transcription	961:973	arg1	proteins					985:992	transcription 6 (STAT6) proteins	961:992	transcription 6 (STAT6) proteins	961:992	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	6	81	theme	MF	1489:1490	arg1	treatment					1492:1500	MF treatment	1489:1500	MF treatment	1489:1500	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	2	82	theme	Sixteen-week-old	494:509	arg1	mice					520:523	Sixteen-week-old IL-10-/- mice	494:523	Sixteen-week-old IL-10-/- mice	494:523	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	4	83	theme	CD25+	1122:1126	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	83	theme	CD25+	1122:1126	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	1	84	theme	interleukin-10	337:350	arg1	mice					373:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	interleukin-10 knockout (IL-10-/- ) mice	337:376	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	7	85	theme	microbial	1780:1788	arg1	butyrate					1802:1809	microbial metabolites butyrate	1780:1809	microbial metabolites butyrate	1780:1809	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	5	86	theme	concentrations	1332:1345	arg1	frequency					1299:1307	decreased lamina propria frequency	1274:1307	decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α)	1274:1428	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	0	87	theme	Nondigestible	8:20	arg1	Polysaccharides					22:36	Dietary Nondigestible Polysaccharides	0:36	Dietary Nondigestible Polysaccharides	0:36	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	3	88	theme	chemoattractant	843:857	arg1	protein-1					859:867	monocyte chemoattractant protein-1	834:867	monocyte chemoattractant protein-1	834:867	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	88	theme	chemoattractant	843:857	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	89	theme	fecal	625:629	arg1	protein-1					859:867	monocyte chemoattractant protein-1	834:867	monocyte chemoattractant protein-1	834:867	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	89	theme	fecal	625:629	arg1	expression					643:652	expression	643:652	expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α]	643:784	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	89	theme	fecal	625:629	arg1	microbiota					631:640	the fecal microbiota	621:640	the fecal microbiota	621:640	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	89	theme	fecal	625:629	arg1	[MIP					825:828	macrophage inflammatory protein [MIP]-2	793:831	macrophage inflammatory protein [MIP]-2	793:831	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	3	89	theme	fecal	625:629	arg1	IL-6					787:790	IL-6	787:790	IL-6	787:790	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	2	90	dep	METHODS	486:492	arg1	used					530:533	used	530:533	were used for the experiments with MF diet for 4 weeks	525:578	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	6	91	theme	gut	1446:1448	arg1	diversity					1460:1468	gut microbial diversity	1446:1468	gut microbial diversity	1446:1468	An increase in gut microbial diversity was observed after MF treatment compared with IL-10-/- mice, including a significant increase in bacteria belonging to the Firmicutes phylum and a significant decrease in bacteria belonging to the Proteobacteria phylum.
30277587	1	92	dep	BACKGROUND	163:172	arg1	was					196:198	was	196:198	was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	196:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	7	93	theme	CD4+	1746:1749	arg1	Tregs					1764:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	CD4+ CD25+ Foxp3+ Tregs	1746:1768	Moreover, MF treatment increased the differentiation of CD4+ CD25+ Foxp3+ Tregs mainly by microbial metabolites butyrate.
30277587	0	94	theme	Gut	70:72	arg1	Microbiota					74:83	Gut Microbiota	70:83	Gut Microbiota	70:83	Dietary Nondigestible Polysaccharides Ameliorate Colitis by Improving Gut Microbiota and CD4+ Differentiation, as Well as Facilitating M2 Macrophage Polarization.
30277587	3	95	theme	experiment	1027:1036	arg1	end					1016:1018	the end	1012:1018	the end of the experiment	1012:1036	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	9	96	theme	treatment	1966:1974	arg1	contribution					1947:1958	the contribution	1943:1958	the contribution of MF treatment to the CD mouse model	1943:1996	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	4	97	from	metabolites	1070:1080	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	97	from	metabolites	1070:1080	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	98	theme	regulatory	1135:1144	arg1	Tregs					1155:1159	Tregs	1155:1159	Tregs	1155:1159	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	98	theme	regulatory	1135:1144	arg1	cells					1148:1152	CD4+ CD25+ Foxp3+ regulatory T cells	1117:1152	CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1117:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	9	99	theme	MF	1963:1964	arg1	treatment					1966:1974	MF treatment	1963:1974	MF treatment	1963:1974	CONCLUSIONS These results shed light on the contribution of MF treatment to the CD mouse model and suggest that MF has potential as a therapeutic strategy for enhancing efficacy in inducing remission in patients with active CD.
30277587	5	100	theme	RESULTS	1203:1209	arg1	treatment					1214:1222	RESULTS MF treatment	1203:1222	RESULTS MF treatment	1203:1222	RESULTS MF treatment significantly ameliorated colitis associated with decreased lamina propria frequency of Th1/Th17 cells, MPO concentrations, and inflammatory cytokines and chemokines (TNF-α, IL-6, MIP-2, MCP-1, and MIP-1α).
30277587	3	101	theme	Th1/Th17	657:664	arg1	cells					666:670	Th1/Th17 cells	657:670	Th1/Th17 cells	657:670	Severity of colitis, the composition of the fecal microbiota, expression of Th1/Th17 cells, myeloperoxidase (MPO) concentrations, and inflammatory cytokines and chemokines (tumor necrosis factor-α [TNF-α], IL-6, macrophage inflammatory protein [MIP]-2, monocyte chemoattractant protein-1 [MCP-1], and MIP-1α), as well as arginase 1 (Arg1) and signal transducers and activators of transcription 6 (STAT6) proteins, were measured at the end of the experiment.
30277587	4	102	located	detected	1172:1179	arg2	metabolites					1070:1080	the corresponding metabolites	1052:1080	the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs)	1052:1160	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	102	located	detected	1172:1179	arg1	addition					1042:1049	addition	1042:1049	addition	1042:1049	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	4	102	located	detected	1172:1179	arg2	acids					1101:1105	short-chain fatty acids	1083:1105	short-chain fatty acids	1083:1105	In addition, the corresponding metabolites (short-chain fatty acids) of MF on CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) were also detected in vivo and in vitro.
30277587	2	103	with	experiments	543:553	arg1	diet					563:566	MF diet	560:566	MF diet for 4 weeks	560:578	METHODS Sixteen-week-old IL-10-/- mice were used for the experiments with MF diet for 4 weeks.
30277587	1	104	theme	specific	246:253	arg1	MF					276:277	MF	276:277	MF	276:277	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
30277587	1	104	theme	specific	246:253	arg1	diet					270:273	a specific multifiber mix diet	244:273	a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD)	244:483	BACKGROUND The aim of this study was to investigate the therapeutic mechanism of a specific multifiber mix diet (MF) designed to match the fiber content of a healthy diet in interleukin-10 knockout (IL-10-/- ) mice with spontaneous chronic colitis displaying similar characteristics to those of human Crohn's disease (CD).
29853356	2	0	theme	oat	387:389	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	6	1	theme	crumb	841:845	arg1	structures					847:856	Homogeneous and heterogeneous crumb structures	811:856	Homogeneous and heterogeneous crumb structures of rye and barley breads	811:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	6	2	theme	high	917:920	arg1	values					932:937	high and low E values	917:937	high and low E values of their respective DLs	917:961	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	5	3	theme	gluten	755:760	arg1	network					762:768	a gluten network	753:768	a gluten network	753:768	Nonetheless, the presence of a gluten network resulted in high-quality wheaten breads.
29853356	6	4	theme	breads	876:881	arg1	structures					847:856	Homogeneous and heterogeneous crumb structures	811:856	Homogeneous and heterogeneous crumb structures of rye and barley breads	811:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	0	5	theme	rye	72:74	arg1	composition					13:23	composition	13:23	composition	13:23	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	0	5	theme	rye	72:74	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	3	6	dep	β-glucan	468:475	arg1	arabinoxylan					454:465	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	arabinoxylan	454:465	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	6	dep	β-glucan	468:475	arg1	lipid					447:451	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	lipid	447:451	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	1	7	theme	wheat	221:225	arg1	dough					227:231	wheat dough	221:231	wheat dough due to weaker protein networks in the former	221:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	6	8	theme	respective	948:957	arg1	DLs					959:961	their respective DLs	942:961	their respective DLs	942:961	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	4	9	theme	DL	653:654	arg1	foaming					626:632	Poor foaming	621:632	Poor foaming	621:632	Poor foaming and low E of wheat DL were ascribed to lipids and proteins co-occurring at the interface.
29853356	4	9	theme	DL	653:654	arg1	E					642:642	low E	638:642	low E	638:642	Poor foaming and low E of wheat DL were ascribed to lipids and proteins co-occurring at the interface.
29853356	7	10	theme	High	964:967	arg1	content					975:981	High lipid content	964:981	High lipid content	964:981	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	0	11	theme	barley	77:82	arg1	composition					13:23	composition	13:23	composition	13:23	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	0	11	theme	barley	77:82	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	3	12	theme	bread	606:610	arg1	quality					612:618	bread quality	606:618	bread quality	606:618	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	7	13	theme	lipid	969:973	arg1	content					975:981	High lipid content	964:981	High lipid content	964:981	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	4	14	theme	low	638:640	arg1	E					642:642	low E	638:642	low E	638:642	Poor foaming and low E of wheat DL were ascribed to lipids and proteins co-occurring at the interface.
29853356	1	15	theme	due	233:235	arg1	dough					227:231	wheat dough	221:231	wheat dough due to weaker protein networks in the former	221:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	0	16	theme	oat	89:91	arg1	liquor					99:104	oat dough liquor	89:104	oat dough liquor	89:104	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	1	17	from	stabilization	116:128	arg1	dough					133:137	dough	133:137	dough	133:137	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	4	18	theme	wheat	647:651	arg1	DL					653:654	wheat DL	647:654	wheat DL	647:654	Poor foaming and low E of wheat DL were ascribed to lipids and proteins co-occurring at the interface.
29853356	6	19	theme	E	930:930	arg1	values					932:937	high and low E values	917:937	high and low E values of their respective DLs	917:961	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	2	20	theme	aqueous	320:326	arg1	phase					328:332	the dough aqueous phase	310:332	the dough aqueous phase	310:332	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	6	21	theme	rye	861:863	arg1	breads					876:881	rye and barley breads	861:881	rye and barley breads	861:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	5	22	theme	network	762:768	arg1	presence					741:748	the presence	737:748	the presence of a gluten network	737:768	Nonetheless, the presence of a gluten network resulted in high-quality wheaten breads.
29853356	1	23	from	networks	255:262	arg1	former					271:276	former	271:276	former	271:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	2	24	theme	Dough	279:283	arg1	DL					293:294	DL	293:294	DL	293:294	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	24	theme	Dough	279:283	arg1	liquor					285:290	Dough liquor	279:290	Dough liquor (DL)	279:295	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	24	theme	Dough	279:283	arg1	model					300:304	a model	298:304	a model for the dough aqueous phase	298:332	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	25	theme	dough	314:318	arg1	phase					328:332	the dough aqueous phase	310:332	the dough aqueous phase	310:332	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	7	26	theme	oat	1111:1113	arg1	breads					1115:1120	oat breads	1111:1120	oat breads	1111:1120	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	4	27	theme	Poor	621:624	arg1	foaming					626:632	Poor foaming	621:632	Poor foaming	621:632	Poor foaming and low E of wheat DL were ascribed to lipids and proteins co-occurring at the interface.
29853356	1	28	theme	aqueous	146:152	arg1	constituents					160:171	its aqueous phase constituents	142:171	its aqueous phase constituents	142:171	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	2	29	theme	wheat	363:367	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	1	30	theme	phase	154:158	arg1	constituents					160:171	its aqueous phase constituents	142:171	its aqueous phase constituents	142:171	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	2	31	theme	fermented	353:361	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	1	32	theme	weaker	240:245	arg1	networks					255:262	weaker protein networks	240:262	weaker protein networks in the former	240:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	0	33	theme	liquor	99:104	arg1	composition					13:23	composition	13:23	composition	13:23	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	0	33	theme	liquor	99:104	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	5	34	theme	wheaten	795:801	arg1	breads					803:808	high-quality wheaten breads	782:808	high-quality wheaten breads	782:808	Nonetheless, the presence of a gluten network resulted in high-quality wheaten breads.
29853356	7	35	theme	DL	1014:1015	arg1	tension					999:1005	low surface tension	987:1005	low surface tension	987:1005	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	7	35	theme	DL	1014:1015	arg1	content					975:981	High lipid content	964:981	High lipid content	964:981	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	1	36	theme	protein	247:253	arg1	networks					255:262	weaker protein networks	240:262	weaker protein networks in the former	240:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	0	37	theme	dough	93:97	arg1	liquor					99:104	oat dough liquor	89:104	oat dough liquor	89:104	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	3	38	dep	composition	425:435	arg1	tension					547:553	surface tension	539:553	surface tension	539:553	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	38	dep	composition	425:435	arg1	[foaming					518:525	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	[foaming	518:525	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	38	dep	composition	425:435	arg1	viscosity					528:536	viscosity	528:536	viscosity	528:536	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	38	dep	composition	425:435	arg1	modulus					577:583	surface dilatational modulus	556:583	surface dilatational modulus (E)	556:587	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	38	dep	composition	425:435	arg1	β-glucan					468:475	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	β-glucan	468:475	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	39	theme	DL	422:423	arg1	composition					425:435	DL composition	422:435	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)]	422:588	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	0	40	theme	interfacial	39:49	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	6	41	theme	heterogeneous	827:839	arg1	structures					847:856	Homogeneous and heterogeneous crumb structures	811:856	Homogeneous and heterogeneous crumb structures of rye and barley breads	811:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	2	42	attach	isolated	339:346	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	42	attach	isolated	339:346	arg2	model					300:304	a model	298:304	a model for the dough aqueous phase	298:332	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	42	attach	isolated	339:346	arg2	DL					293:294	DL	293:294	DL	293:294	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	2	42	attach	isolated	339:346	arg2	liquor					285:290	Dough liquor	279:290	Dough liquor (DL)	279:295	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	3	43	theme	air/water	482:490	arg1	[foaming					518:525	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	[foaming	518:525	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	6	44	theme	low	926:928	arg1	values					932:937	high and low E values	917:937	high and low E values of their respective DLs	917:961	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	0	45	theme	air/water	29:37	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	2	46	theme	barley	375:380	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	6	47	theme	barley	869:874	arg1	breads					876:881	rye and barley breads	861:881	rye and barley breads	861:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	3	48	theme	interfacial	492:502	arg1	[foaming					518:525	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	[foaming	518:525	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	7	49	theme	low	987:989	arg1	tension					999:1005	low surface tension	987:1005	low surface tension	987:1005	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	6	50	theme	Homogeneous	811:821	arg1	structures					847:856	Homogeneous and heterogeneous crumb structures	811:856	Homogeneous and heterogeneous crumb structures of rye and barley breads	811:881	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	1	51	from	non-wheat	203:211	arg1	important					190:198	important	190:198	important	190:198	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	3	52	theme	surface	539:545	arg1	tension					547:553	surface tension	539:553	surface tension	539:553	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	52	theme	surface	539:545	arg1	β-glucan					468:475	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	β-glucan	468:475	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	2	53	theme	rye	370:372	arg1	doughs					391:396	fermented wheat, rye, barley, and oat doughs	353:396	fermented wheat, rye, barley, and oat doughs	353:396	Dough liquor (DL), a model for the dough aqueous phase, was isolated from fermented wheat, rye, barley, and oat doughs by ultracentrifugation.
29853356	1	54	from	dough	227:231	arg1	important					190:198	important	190:198	important	190:198	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	7	55	theme	oat	1010:1012	arg1	DL					1014:1015	oat DL	1010:1015	oat DL	1010:1015	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	1	56	from	important	190:198	arg1	non-wheat					203:211	non-wheat	203:211	non-wheat	203:211	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	1	56	from	important	190:198	arg1	dough					227:231	wheat dough	221:231	wheat dough due to weaker protein networks in the former	221:276	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	7	57	theme	surface	991:997	arg1	tension					999:1005	low surface tension	987:1005	low surface tension	987:1005	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	5	58	theme	high-quality	782:793	arg1	breads					803:808	high-quality wheaten breads	782:808	high-quality wheaten breads	782:808	Nonetheless, the presence of a gluten network resulted in high-quality wheaten breads.
29853356	3	59	theme	protein	438:444	arg1	tension					547:553	surface tension	539:553	surface tension	539:553	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	59	theme	protein	438:444	arg1	viscosity					528:536	viscosity	528:536	viscosity	528:536	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	59	theme	protein	438:444	arg1	modulus					577:583	surface dilatational modulus	556:583	surface dilatational modulus (E)	556:587	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	59	theme	protein	438:444	arg1	β-glucan					468:475	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	β-glucan	468:475	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	60	theme	surface	556:562	arg1	E					586:586	E	586:586	E	586:586	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	60	theme	surface	556:562	arg1	modulus					577:583	surface dilatational modulus	556:583	surface dilatational modulus (E)	556:587	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	60	theme	surface	556:562	arg1	β-glucan					468:475	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	β-glucan	468:475	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	0	61	dep	composition	13:23	arg1	the					9:11	the	9:11	the	9:11	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	1	62	theme	Gas	107:109	arg1	stabilization					116:128	Gas cell stabilization	107:128	Gas cell stabilization in dough by its aqueous phase constituents	107:171	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	7	63	theme	breads	1115:1120	arg1	structure					1098:1106	the heterogeneous crumb structure	1074:1106	the heterogeneous crumb structure of oat breads	1074:1120	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	3	64	theme	dilatational	564:575	arg1	E					586:586	E	586:586	E	586:586	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	64	theme	dilatational	564:575	arg1	modulus					577:583	surface dilatational modulus	556:583	surface dilatational modulus (E)	556:587	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	64	theme	dilatational	564:575	arg1	β-glucan					468:475	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	β-glucan	468:475	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	3	65	theme	functionality	504:516	arg1	[foaming					518:525	protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming	438:525	[foaming	518:525	DL composition (protein, lipid, arabinoxylan, β-glucan) and air/water interfacial functionality [foaming, viscosity, surface tension, surface dilatational modulus (E)] were related to bread quality.
29853356	1	66	theme	cell	111:114	arg1	stabilization					116:128	Gas cell stabilization	107:128	Gas cell stabilization in dough by its aqueous phase constituents	107:171	Gas cell stabilization in dough by its aqueous phase constituents is arguably more important in non-wheat than in wheat dough due to weaker protein networks in the former.
29853356	7	67	theme	heterogeneous	1078:1090	arg1	structure					1098:1106	the heterogeneous crumb structure	1074:1106	the heterogeneous crumb structure of oat breads	1074:1120	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	0	68	theme	wheat	65:69	arg1	composition					13:23	composition	13:23	composition	13:23	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	0	68	theme	wheat	65:69	arg1	properties					51:60	air/water interfacial properties	29:60	air/water interfacial properties	29:60	Relating the composition and air/water interfacial properties of wheat, rye, barley, and oat dough liquor.
29853356	7	69	theme	lipid-dominated	1029:1043	arg1	interface					1045:1053	a lipid-dominated interface	1027:1053	a lipid-dominated interface	1027:1053	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
29853356	6	70	theme	DLs	959:961	arg1	values					932:937	high and low E values	917:937	high and low E values of their respective DLs	917:961	Homogeneous and heterogeneous crumb structures of rye and barley breads, respectively, were attributed to high and low E values of their respective DLs.
29853356	7	71	theme	crumb	1092:1096	arg1	structure					1098:1106	the heterogeneous crumb structure	1074:1106	the heterogeneous crumb structure of oat breads	1074:1120	High lipid content and low surface tension of oat DL indicated a lipid-dominated interface, which may explain the heterogeneous crumb structure of oat breads.
30521915	1	0	theme	CS/P123/PdCl2	368:380	arg1	membranes					398:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	8	1	theme	Pd	1555:1556	arg1	composites					1572:1581	Pd@N-C catalytic composites	1555:1581	Pd@N-C catalytic composites with desirable Pd loading	1555:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	1	2	theme	N2	436:437	arg1	atmosphere					439:448	N2 atmosphere	436:448	N2 atmosphere	436:448	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	1	3	theme	membranes	398:406	arg1	carbonization					256:268	one-pot thermal carbonization	240:268	one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	240:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	8	4	theme	N-C	1558:1560	arg1	composites					1572:1581	Pd@N-C catalytic composites	1555:1581	Pd@N-C catalytic composites with desirable Pd loading	1555:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	6	5	theme	carbon	1192:1197	arg1	matrix					1178:1183	the porous matrix	1167:1183	the porous matrix of the carbon	1167:1197	Pd nanoparticles sized in 5-20 nm dispersed well on the porous matrix of the carbon.
30521915	1	6	theme	Pd	194:195	arg1	N-C					197:199	Pd@N-C	194:199	Pd@N-C	194:199	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	1	6	theme	Pd	194:195	arg1	Pd					190:191	Pd	190:191	Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	190:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	1	7	theme	ethylene	287:294	arg1	glycol					296:301	ethylene glycol	287:301	ethylene glycol	287:301	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	6	8	theme	porous	1171:1176	arg1	matrix					1178:1183	the porous matrix	1167:1183	the porous matrix of the carbon	1167:1197	Pd nanoparticles sized in 5-20 nm dispersed well on the porous matrix of the carbon.
30521915	1	9	theme	catalytic	202:210	arg1	composites					212:221	catalytic composites	202:221	catalytic composites	202:221	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	7	10	theme	halides	1312:1318	arg1	reactions					1290:1298	the Ullmann homo-coupling reactions	1264:1298	the Ullmann homo-coupling reactions of aromatic halides	1264:1318	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	1	11	theme	hydrogel	389:396	arg1	membranes					398:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	3	12	theme	highest	586:592	arg1	temperature					608:618	highest carbonization temperature	586:618	highest carbonization temperature of 900 °C	586:628	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	2	13	theme	carbonization	556:568	arg1	temperature					570:580	the carbonization temperature	552:580	the carbonization temperature	552:580	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	8	14	theme	one-pot	1363:1369	arg1	process					1385:1391	such one-pot carbonization process	1358:1391	such one-pot carbonization process	1358:1391	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	15	from	membrane	696:703	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	1	16	theme	different	411:419	arg1	temperature					421:431	different temperature	411:431	different temperature	411:431	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	7	17	theme	homo-coupling	1276:1288	arg1	reactions					1290:1298	the Ullmann homo-coupling reactions	1264:1298	the Ullmann homo-coupling reactions of aromatic halides	1264:1318	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	3	18	theme	pores	779:783	arg1	SBET					739:742	SBET	739:742	SBET	739:742	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	18	theme	pores	779:783	arg1	area					733:736	the highest specific area	712:736	the highest specific area (SBET) of 293.7 m2/g	712:757	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	18	theme	pores	779:783	arg1	volume					769:774	total volume	763:774	total volume of pores (Vtot) of 0.79 cm3/g	763:804	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	18	theme	pores	779:783	arg1	Vtot					786:789	Vtot	786:789	Vtot	786:789	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	5	19	theme	spectrum	975:982	arg1	results					984:990	Raman spectrum results	969:990	Raman spectrum results	969:990	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	3	20	theme	900 °C	623:628	arg1	temperature					608:618	highest carbonization temperature	586:618	highest carbonization temperature of 900 °C	586:628	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	1	21	theme	porous	151:156	arg1	carbon					173:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	2	22	theme	catalytic	486:494	arg1	composite					496:504	the Pd@N-C catalytic composite	475:504	the Pd@N-C catalytic composite	475:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	0	23	theme	N-doped	73:79	arg1	carbon					81:86	N-doped carbon	73:86	N-doped carbon	73:86	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	1	24	theme	‑block‑poly	332:342	arg1	membranes					398:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	2	25	theme	@	481:481	arg1	composite					496:504	the Pd@N-C catalytic composite	475:504	the Pd@N-C catalytic composite	475:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	1	26	theme	ethylene	344:351	arg1	glycol					353:358	ethylene glycol	344:358	ethylene glycol	344:358	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	4	27	theme	Pd	868:869	arg1	composites					885:894	the Pd@N-C catalytic composites	864:894	the Pd@N-C catalytic composites	864:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	0	28	theme	catalytic	101:109	arg1	composites					111:120	Pd catalytic composites	98:120	Pd catalytic composites for Ullmann reactions	98:142	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	5	29	theme	Pd	1017:1018	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	4	30	theme	N-C	871:873	arg1	composites					885:894	the Pd@N-C catalytic composites	864:894	the Pd@N-C catalytic composites	864:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	5	31	theme	N-C	1020:1022	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	8	32	theme	etching	1459:1465	arg1	steps					1479:1483	further etching and removal steps	1451:1483	further etching and removal steps	1451:1483	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	0	33	theme	One-pot	0:6	arg1	carbonization					8:20	One-pot carbonization	0:20	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon	0:86	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	5	34	theme	graphite	1098:1105	arg1	carbon					1107:1112	some graphite carbon	1093:1112	some graphite carbon	1093:1112	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	7	35	theme	excellent	1239:1247	arg1	activities					1249:1258	excellent activities	1239:1258	excellent activities for the Ullmann homo-coupling reactions of aromatic halides	1239:1318	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	8	36	theme	removal	1471:1477	arg1	steps					1479:1483	further etching and removal steps	1451:1483	further etching and removal steps	1451:1483	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	4	37	theme	X-ray	916:920	arg1	XPS					950:952	XPS	950:952	XPS	950:952	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	4	37	theme	X-ray	916:920	arg1	spectroscopy					936:947	X-ray photoelectron spectroscopy	916:947	X-ray photoelectron spectroscopy (XPS) measurements	916:966	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	8	38	with	composites	1572:1581	arg1	loading					1601:1607	desirable Pd loading	1588:1607	desirable Pd loading	1588:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	39	theme	Pd	644:645	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	0	40	theme	blend	45:49	arg1	membranes					60:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	7	41	theme	N-C	1207:1209	arg1	composites					1221:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	4	42	theme	spectroscopy	936:947	arg1	measurements					955:966	X-ray photoelectron spectroscopy (XPS) measurements	916:966	X-ray photoelectron spectroscopy (XPS) measurements	916:966	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	2	43	theme	N-C	482:484	arg1	composite					496:504	the Pd@N-C catalytic composite	475:504	the Pd@N-C catalytic composite	475:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	0	44	theme	membranes	60:68	arg1	carbonization					8:20	One-pot carbonization	0:20	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon	0:86	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	7	45	theme	Pd	1204:1205	arg1	composites					1221:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	8	46	theme	preparation	1507:1517	arg1	process					1519:1525	the preparation process	1503:1525	the preparation process	1503:1525	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	8	47	theme	catalytic	1562:1570	arg1	composites					1572:1581	Pd@N-C catalytic composites	1555:1581	Pd@N-C catalytic composites with desirable Pd loading	1555:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	48	theme	N-C	647:649	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	1	49	theme	one-pot	240:246	arg1	carbonization					256:268	one-pot thermal carbonization	240:268	one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	240:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	5	50	contain	contained	1044:1052	arg2	carbon					1072:1077	mainly disordered carbon	1054:1077	mainly disordered carbon together with some graphite carbon	1054:1112	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	5	50	contain	contained	1044:1052	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	8	51	theme	Pd	1598:1599	arg1	loading					1601:1607	desirable Pd loading	1588:1607	desirable Pd loading	1588:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	52	theme	CS/P123/PdCl2	676:688	arg1	membrane					696:703	CS/P123/PdCl2 blend membrane	676:703	CS/P123/PdCl2 blend membrane	676:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	4	53	theme	elements	833:840	arg1	state					820:824	The chemical state	807:824	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites	807:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	8	54	theme	@	1557:1557	arg1	composites					1572:1581	Pd@N-C catalytic composites	1555:1581	Pd@N-C catalytic composites with desirable Pd loading	1555:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	55	theme	specific	724:731	arg1	SBET					739:742	SBET	739:742	SBET	739:742	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	55	theme	specific	724:731	arg1	area					733:736	the highest specific area	712:736	the highest specific area (SBET) of 293.7 m2/g	712:757	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	2	56	theme	porous	455:460	arg1	structure					462:470	The porous structure	451:470	The porous structure of the Pd@N-C catalytic composite	451:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	1	57	theme	@	196:196	arg1	N-C					197:199	Pd@N-C	194:199	Pd@N-C	194:199	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	1	57	theme	@	196:196	arg1	Pd					190:191	Pd	190:191	Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	190:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	4	58	theme	chemical	811:818	arg1	state					820:824	The chemical state	807:824	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites	807:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	8	59	theme	such	1358:1361	arg1	process					1385:1391	such one-pot carbonization process	1358:1391	such one-pot carbonization process	1358:1391	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	1	60	theme	blend	383:387	arg1	membranes					398:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	8	61	theme	carbonization	1371:1383	arg1	process					1385:1391	such one-pot carbonization process	1358:1391	such one-pot carbonization process	1358:1391	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	62	theme	total	763:767	arg1	volume					769:774	total volume	763:774	total volume of pores (Vtot) of 0.79 cm3/g	763:804	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	62	theme	total	763:767	arg1	Vtot					786:789	Vtot	786:789	Vtot	786:789	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	5	63	theme	prepared	1008:1015	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	4	64	theme	C	845:845	arg1	elements					833:840	the elements	829:840	the elements of C, N, O, Pd within the Pd@N-C catalytic composites	829:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	1	65	dep	chitosan/poly	273:285	arg1	glycol					325:330	propylene glycol	315:330	propylene glycol	315:330	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	1	65	dep	chitosan/poly	273:285	arg1	glycol					296:301	ethylene glycol	287:301	ethylene glycol	287:301	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	7	66	theme	aromatic	1303:1310	arg1	halides					1312:1318	aromatic halides	1303:1318	aromatic halides	1303:1318	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	6	67	dep	nanoparticles	1118:1130	arg1	sized					1132:1136	sized	1132:1136	nanoparticles sized in 5-20 nm dispersed well on the porous matrix of the carbon	1118:1197	Pd nanoparticles sized in 5-20 nm dispersed well on the porous matrix of the carbon.
30521915	3	68	theme	293.7 m2/g	748:757	arg1	SBET					739:742	SBET	739:742	SBET	739:742	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	68	theme	293.7 m2/g	748:757	arg1	area					733:736	the highest specific area	712:736	the highest specific area (SBET) of 293.7 m2/g	712:757	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	68	theme	293.7 m2/g	748:757	arg1	volume					769:774	total volume	763:774	total volume of pores (Vtot) of 0.79 cm3/g	763:804	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	68	theme	293.7 m2/g	748:757	arg1	Vtot					786:789	Vtot	786:789	Vtot	786:789	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	5	69	theme	Raman	969:973	arg1	results					984:990	Raman spectrum results	969:990	Raman spectrum results	969:990	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	1	70	theme	Novel	145:149	arg1	carbon					173:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	2	71	theme	P123	543:546	arg1	addition					531:538	the addition	527:538	the addition of P123	527:546	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	2	71	theme	P123	543:546	arg1	temperature					570:580	the carbonization temperature	552:580	the carbonization temperature	552:580	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	8	72	theme	polymer	1398:1404	arg1	beneficial					1534:1543	beneficial	1534:1543	beneficial	1534:1543	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	8	72	theme	polymer	1398:1404	arg1	porogen					1406:1412	the polymer porogen	1394:1412	the polymer porogen	1394:1412	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	73	theme	carbonization	594:606	arg1	temperature					608:618	highest carbonization temperature	586:618	highest carbonization temperature of 900 °C	586:628	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	1	74	theme	nitrogen-doped	158:171	arg1	carbon					173:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon	145:178	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	4	75	theme	catalytic	875:883	arg1	composites					885:894	the Pd@N-C catalytic composites	864:894	the Pd@N-C catalytic composites	864:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	7	76	theme	Ullmann	1268:1274	arg1	reactions					1290:1298	the Ullmann homo-coupling reactions	1264:1298	the Ullmann homo-coupling reactions of aromatic halides	1264:1318	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	0	77	theme	Pd	98:99	arg1	composites					111:120	Pd catalytic composites	98:120	Pd catalytic composites for Ullmann reactions	98:142	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	2	78	theme	Pd	479:480	arg1	composite					496:504	the Pd@N-C catalytic composite	475:504	the Pd@N-C catalytic composite	475:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	3	79	theme	0.79 cm3/g	795:804	arg1	SBET					739:742	SBET	739:742	SBET	739:742	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	79	theme	0.79 cm3/g	795:804	arg1	area					733:736	the highest specific area	712:736	the highest specific area (SBET) of 293.7 m2/g	712:757	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	79	theme	0.79 cm3/g	795:804	arg1	volume					769:774	total volume	763:774	total volume of pores (Vtot) of 0.79 cm3/g	763:804	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	79	theme	0.79 cm3/g	795:804	arg1	Vtot					786:789	Vtot	786:789	Vtot	786:789	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	5	80	theme	disordered	1061:1070	arg1	carbon					1072:1077	mainly disordered carbon	1054:1077	mainly disordered carbon together with some graphite carbon	1054:1112	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	8	81	theme	further	1451:1457	arg1	steps					1479:1483	further etching and removal steps	1451:1483	further etching and removal steps	1451:1483	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	0	82	theme	Ullmann	126:132	arg1	reactions					134:142	Ullmann reactions	126:142	Ullmann reactions	126:142	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	1	83	theme	/PdCl2	360:365	arg1	membranes					398:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	273:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	4	84	theme	@	870:870	arg1	composites					885:894	the Pd@N-C catalytic composites	864:894	the Pd@N-C catalytic composites	864:894	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	5	85	theme	catalytic	1024:1032	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	5	86	theme	@	1019:1019	arg1	composite					1034:1042	the prepared Pd@N-C catalytic composite	1004:1042	the prepared Pd@N-C catalytic composite	1004:1042	Raman spectrum results showed that the prepared Pd@N-C catalytic composite contained mainly disordered carbon together with some graphite carbon.
30521915	1	87	dep	Pd	190:191	arg1	prepared					228:235	prepared	228:235	were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	223:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	0	88	theme	chitosan/P123/PdCl2	25:43	arg1	membranes					60:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	7	89	theme	catalytic	1211:1219	arg1	composites					1221:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	1	90	dep	‑block‑poly	332:342	arg1	glycol					353:358	ethylene glycol	344:358	ethylene glycol	344:358	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	3	91	theme	prepared	635:642	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	0	92	theme	hydrogel	51:58	arg1	membranes					60:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	chitosan/P123/PdCl2 blend hydrogel membranes	25:68	One-pot carbonization of chitosan/P123/PdCl2 blend hydrogel membranes to N-doped carbon supported Pd catalytic composites for Ullmann reactions.
30521915	7	93	theme	@	1206:1206	arg1	composites					1221:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites	1200:1230	The Pd@N-C catalytic composites showed excellent activities for the Ullmann homo-coupling reactions of aromatic halides, and can be recycled for 10 times.
30521915	2	94	theme	composite	496:504	arg1	structure					462:470	The porous structure	451:470	The porous structure of the Pd@N-C catalytic composite	451:504	The porous structure of the Pd@N-C catalytic composite was governed by both the addition of P123 and the carbonization temperature.
30521915	1	95	theme	propylene	315:323	arg1	glycol					325:330	propylene glycol	315:330	propylene glycol	315:330	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
30521915	4	96	theme	photoelectron	922:934	arg1	XPS					950:952	XPS	950:952	XPS	950:952	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	4	96	theme	photoelectron	922:934	arg1	spectroscopy					936:947	X-ray photoelectron spectroscopy	916:947	X-ray photoelectron spectroscopy (XPS) measurements	916:966	The chemical state of the elements of C, N, O, Pd within the Pd@N-C catalytic composites were confirmed with X-ray photoelectron spectroscopy (XPS) measurements.
30521915	3	97	theme	catalytic	651:659	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	98	dep	specific	724:731	arg1	highest					716:722	highest	716:722	highest	716:722	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	3	99	theme	@	646:646	arg1	composite					661:669	the prepared Pd@N-C catalytic composite	631:669	the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane	631:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	8	100	theme	desirable	1588:1596	arg1	loading					1601:1607	desirable Pd loading	1588:1607	desirable Pd loading	1588:1607	In such one-pot carbonization process, the polymer porogen is simultaneously decomposed without further etching and removal steps, which simplifies the preparation process and is beneficial to obtain Pd@N-C catalytic composites with desirable Pd loading.
30521915	3	101	theme	blend	690:694	arg1	membrane					696:703	CS/P123/PdCl2 blend membrane	676:703	CS/P123/PdCl2 blend membrane	676:703	At highest carbonization temperature of 900 °C, the prepared Pd@N-C catalytic composite from CS/P123/PdCl2 blend membrane showed the highest specific area (SBET) of 293.7 m2/g and total volume of pores (Vtot) of 0.79 cm3/g.
30521915	1	102	theme	thermal	248:254	arg1	carbonization					256:268	one-pot thermal carbonization	240:268	one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes	240:406	Novel porous nitrogen-doped carbon supported Pd (Pd@N-C) catalytic composites were prepared by one-pot thermal carbonization of chitosan/poly(ethylene glycol)‑block‑poly(propylene glycol)‑block‑poly(ethylene glycol)/PdCl2 (CS/P123/PdCl2) blend hydrogel membranes at different temperature in N2 atmosphere.
31569331	0	0	theme	Polysaccharides	83:97	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	0	theme	Polysaccharides	83:97	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	3	1	theme	Fe2+	732:735	arg1	radicals					691:698	hydroxyl radicals	682:698	hydroxyl radicals	682:698	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	3	1	theme	Fe2+	732:735	arg1	DPPH					675:678	DPPH	675:678	DPPH	675:678	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	3	1	theme	Fe2+	732:735	arg1	activities					718:727	the chelating activities	704:727	the chelating activities of Fe2+	704:735	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	3	1	theme	Fe2+	732:735	arg1	2-diphenylpicrylhydrazyl					649:672	2, 2-diphenylpicrylhydrazyl	646:672	2-diphenylpicrylhydrazyl	649:672	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	1	2	theme	physiological	170:182	arg1	roles					283:287	important roles	273:287	important roles	273:287	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	2	theme	physiological	170:182	arg1	process					184:190	an irreversible and complex degenerative physiological process	129:190	an irreversible and complex degenerative physiological process	129:190	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	3	theme	important	273:281	arg1	roles					283:287	important roles	273:287	important roles	273:287	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	3	theme	important	273:281	arg1	process					184:190	an irreversible and complex degenerative physiological process	129:190	an irreversible and complex degenerative physiological process	129:190	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	0	4	theme	Sulfated	74:81	arg1	Polysaccharides					83:97	Sulfated Polysaccharides	74:97	Sulfated Polysaccharides from Flammulina velutipes	74:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	3	5	theme	chelating	708:716	arg1	activities					718:727	the chelating activities	704:727	the chelating activities of Fe2+	704:735	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	4	6	theme	enzyme	915:920	arg1	activities					922:931	the antioxidant enzyme activities	899:931	the antioxidant enzyme activities	899:931	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	5	7	with	heteropolysaccharide	1202:1221	arg1	weight					1255:1260	a weight-average molecular weight	1228:1260	a weight-average molecular weight of 2.81 × 103 Da	1228:1277	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	3	8	theme	in	530:531	arg1	activities					551:560	The in vitro antioxidant activities	526:560	The in vitro antioxidant activities	526:560	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	5	9	theme	2.81	1265:1268	arg1	×					1270:1270	×	1270:1270	×	1270:1270	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	0	10	theme	Flammulina	104:113	arg1	velutipes					115:123	Flammulina velutipes	104:123	Flammulina velutipes	104:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	7	11	theme	sulfated	1428:1435	arg1	modification					1437:1448	sulfated modification	1428:1448	sulfated modification	1428:1448	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	6	12	theme	characteristic	1318:1331	arg1	groups					1344:1349	characteristic functional groups	1318:1349	characteristic functional groups	1318:1349	Furthermore, SFPS has also changed in characteristic functional groups and monosaccharide composition compared to FPS.
31569331	5	13	theme	weight-average	1230:1243	arg1	weight					1255:1260	a weight-average molecular weight	1228:1260	a weight-average molecular weight of 2.81 × 103 Da	1228:1277	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	7	14	theme	anti-aging	1484:1493	arg1	activities					1510:1519	the anti-oxidation, anti-aging and protective activities	1464:1519	the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines	1464:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	7	15	theme	F.	1524:1525	arg1	polysaccharides					1537:1551	F. velutipes polysaccharides	1524:1551	F. velutipes polysaccharides	1524:1551	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	2	16	theme	anti-aging	377:386	arg1	activities					388:397	the antioxidant and anti-aging activities	357:397	the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice	357:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	4	17	theme	in	742:743	arg1	experiments					757:767	The in vivo animal experiments	738:767	The in vivo animal experiments manifested that the SFPS	738:792	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	4	18	theme	animal	750:755	arg1	experiments					757:767	The in vivo animal experiments	738:767	The in vivo animal experiments manifested that the SFPS	738:792	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	5	19	theme	103	1272:1274	arg1	×					1270:1270	×	1270:1270	×	1270:1270	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	4	20	theme	superior	801:808	arg1	abilities					837:845	superior antioxidant and protective abilities	801:845	superior antioxidant and protective abilities	801:845	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	4	21	theme	anile	1022:1026	arg1	condition					1028:1036	the anile condition	1018:1036	the anile condition of mice	1018:1044	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	7	22	theme	velutipes	1527:1535	arg1	polysaccharides					1537:1551	F. velutipes polysaccharides	1524:1551	F. velutipes polysaccharides	1524:1551	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	2	23	theme	polysaccharides	423:437	arg1	activities					388:397	the antioxidant and anti-aging activities	357:397	the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice	357:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	7	24	theme	polysaccharides	1537:1551	arg1	activities					1510:1519	the anti-oxidation, anti-aging and protective activities	1464:1519	the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines	1464:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	4	25	theme	antioxidant	903:913	arg1	activities					922:931	the antioxidant enzyme activities	899:931	the antioxidant enzyme activities	899:931	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	3	26	theme	reducing	596:603	arg1	power					605:609	strong reducing power and superior scavenging effects	589:641	power	605:609	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	2	27	theme	velutipes	413:421	arg1	polysaccharides					423:437	Flammulina velutipes polysaccharides	402:437	Flammulina velutipes polysaccharides (FPS)	402:443	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	2	27	theme	velutipes	413:421	arg1	FPS					440:442	FPS	440:442	FPS	440:442	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	3	28	dep	in	530:531	arg1	vitro					533:537	vitro	533:537	vitro	533:537	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	2	29	theme	Flammulina	402:411	arg1	polysaccharides					423:437	Flammulina velutipes polysaccharides	402:437	Flammulina velutipes polysaccharides (FPS)	402:443	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	2	29	theme	Flammulina	402:411	arg1	FPS					440:442	FPS	440:442	FPS	440:442	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	2	30	theme	FPS	462:464	arg1	activities					388:397	the antioxidant and anti-aging activities	357:397	the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice	357:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	3	31	contain	had	585:587	arg2	power					605:609	strong reducing power and superior scavenging effects	589:641	power	605:609	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	3	31	contain	had	585:587	arg1	SFPS					580:583	SFPS	580:583	SFPS	580:583	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	3	31	contain	had	585:587	arg2	effects					635:641	strong reducing power and superior scavenging effects	589:641	effects	635:641	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	4	32	theme	inflammatory	979:990	arg1	response					992:999	the inflammatory response	975:999	the inflammatory response	975:999	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	2	33	from	activities	388:397	arg1	mice					502:505	d-galactose-induced aging mice	476:505	d-galactose-induced aging mice	476:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	7	34	theme	anti-oxidation	1468:1481	arg1	activities					1510:1519	the anti-oxidation, anti-aging and protective activities	1464:1519	the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines	1464:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	0	35	theme	Antioxidant	18:28	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	35	theme	Antioxidant	18:28	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	5	36	theme	molecular	1245:1253	arg1	weight					1255:1260	a weight-average molecular weight	1228:1260	a weight-average molecular weight of 2.81 × 103 Da	1228:1277	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	7	37	theme	protective	1499:1508	arg1	activities					1510:1519	the anti-oxidation, anti-aging and protective activities	1464:1519	the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines	1464:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	7	38	theme	natural	1627:1633	arg1	medicines					1635:1643	natural medicines	1627:1643	natural medicines	1627:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	3	39	theme	strong	589:594	arg1	power					605:609	strong reducing power and superior scavenging effects	589:641	power	605:609	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	2	40	theme	aging	496:500	arg1	mice					502:505	d-galactose-induced aging mice	476:505	d-galactose-induced aging mice	476:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	1	41	theme	abundant	318:325	arg1	bioactivities					327:339	their abundant bioactivities	312:339	their abundant bioactivities	312:339	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	7	42	theme	medicines	1635:1643	arg1	development					1591:1601	the development	1587:1601	the development of functional foods and natural medicines	1587:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	3	43	theme	scavenging	624:633	arg1	effects					635:641	strong reducing power and superior scavenging effects	589:641	effects	635:641	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	7	44	theme	functional	1606:1615	arg1	foods					1617:1621	functional foods	1606:1621	functional foods	1606:1621	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	0	45	theme	Anti-Aging	31:40	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	45	theme	Anti-Aging	31:40	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	6	46	theme	functional	1333:1342	arg1	groups					1344:1349	characteristic functional groups	1318:1349	characteristic functional groups	1318:1349	Furthermore, SFPS has also changed in characteristic functional groups and monosaccharide composition compared to FPS.
31569331	2	47	theme	d-galactose-induced	476:494	arg1	mice					502:505	d-galactose-induced aging mice	476:505	d-galactose-induced aging mice	476:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	5	48	theme	HPLC	1133:1136	arg1	analysis					1138:1145	HPLC analysis	1133:1145	HPLC analysis	1133:1145	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	5	49	theme	SFPS	1087:1090	arg1	analysis					1075:1082	the structural analysis	1060:1082	the structural analysis of SFPS	1060:1090	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	0	50	theme	Protective	52:61	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	50	theme	Protective	52:61	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	5	51	theme	homogeneous	1190:1200	arg1	heteropolysaccharide					1202:1221	a homogeneous heteropolysaccharide	1188:1221	a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da	1188:1277	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	5	51	theme	homogeneous	1190:1200	arg1	SFPS					1179:1182	SFPS	1179:1182	SFPS	1179:1182	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	4	52	theme	d-galactose-induced	859:877	arg1	aging					879:883	the d-galactose-induced aging	855:883	the d-galactose-induced aging	855:883	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	0	53	from	Effects	63:69	arg1	velutipes					115:123	Flammulina velutipes	104:123	Flammulina velutipes	104:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	2	54	theme	antioxidant	361:371	arg1	activities					388:397	the antioxidant and anti-aging activities	357:397	the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice	357:505	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	0	55	theme	Organ	46:50	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	55	theme	Organ	46:50	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	4	56	theme	lipid	945:949	arg1	peroxidation					951:962	lipid peroxidation	945:962	lipid peroxidation	945:962	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	3	57	theme	superior	615:622	arg1	effects					635:641	strong reducing power and superior scavenging effects	589:641	effects	635:641	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	7	58	theme	foods	1617:1621	arg1	development					1591:1601	the development	1587:1601	the development of functional foods and natural medicines	1587:1643	These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.
31569331	4	59	theme	protective	826:835	arg1	abilities					837:845	superior antioxidant and protective abilities	801:845	superior antioxidant and protective abilities	801:845	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	6	60	theme	monosaccharide	1355:1368	arg1	composition					1370:1380	monosaccharide composition	1355:1380	monosaccharide composition	1355:1380	Furthermore, SFPS has also changed in characteristic functional groups and monosaccharide composition compared to FPS.
31569331	0	61	from	velutipes	115:123	arg1	Effects					63:69	Antioxidant, Anti-Aging and Organ Protective Effects	18:69	Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes	18:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	61	from	velutipes	115:123	arg1	Polysaccharides					83:97	Sulfated Polysaccharides	74:97	Sulfated Polysaccharides from Flammulina velutipes	74:123	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	0	61	from	velutipes	115:123	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes.
31569331	1	62	theme	irreversible	132:143	arg1	roles					283:287	important roles	273:287	important roles	273:287	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	62	theme	irreversible	132:143	arg1	process					184:190	an irreversible and complex degenerative physiological process	129:190	an irreversible and complex degenerative physiological process	129:190	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	5	63	theme	Da	1276:1277	arg1	weight					1255:1260	a weight-average molecular weight	1228:1260	a weight-average molecular weight of 2.81 × 103 Da	1228:1277	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	3	64	theme	hydroxyl	682:689	arg1	radicals					691:698	hydroxyl radicals	682:698	hydroxyl radicals	682:698	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	4	65	theme	antioxidant	810:820	arg1	abilities					837:845	superior antioxidant and protective abilities	801:845	superior antioxidant and protective abilities	801:845	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	3	66	theme	antioxidant	539:549	arg1	activities					551:560	The in vitro antioxidant activities	526:560	The in vitro antioxidant activities	526:560	The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+.
31569331	4	67	dep	in	742:743	arg1	vivo					745:748	vivo	745:748	vivo	745:748	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	4	68	theme	mice	1041:1044	arg1	condition					1028:1036	the anile condition	1018:1036	the anile condition of mice	1018:1044	The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice.
31569331	5	69	theme	structural	1064:1073	arg1	analysis					1075:1082	the structural analysis	1060:1082	the structural analysis of SFPS	1060:1090	Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da.
31569331	1	70	theme	complex	149:155	arg1	roles					283:287	important roles	273:287	important roles	273:287	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	70	theme	complex	149:155	arg1	process					184:190	an irreversible and complex degenerative physiological process	129:190	an irreversible and complex degenerative physiological process	129:190	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	2	71	theme	sulfated	453:460	arg1	SFPS					467:470	SFPS	467:470	SFPS	467:470	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	2	71	theme	sulfated	453:460	arg1	FPS					462:464	its sulfated FPS	449:464	its sulfated FPS (SFPS)	449:471	In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated.
31569331	1	72	theme	degenerative	157:168	arg1	roles					283:287	important roles	273:287	important roles	273:287	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
31569331	1	72	theme	degenerative	157:168	arg1	process					184:190	an irreversible and complex degenerative physiological process	129:190	an irreversible and complex degenerative physiological process	129:190	As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities.
29727641	0	0	from	study	14:18	arg1	properties					27:36	the properties	23:36	the properties of graphene oxide	23:54	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	7	1	from	enhancement	1177:1187	arg1	properties					1209:1218	water resistance properties	1192:1218	water resistance properties of GO and AC treated composites	1192:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	5	2	dep	GO	893:894	arg1	composites					911:920	treated composites	903:920	treated composites	903:920	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	5	2	dep	GO	893:894	arg1	The					889:891	The	889:891	The	889:891	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	2	3	theme	starch	410:415	arg1	polymer					417:423	the starch polymer	406:423	the starch polymer	406:423	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	1	4	theme	solution	294:301	arg1	technique					312:320	the solution blending technique	290:320	the solution blending technique using water as a solvent	290:345	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	6	5	theme	AC	1106:1107	arg1	addition					1088:1095	addition	1088:1095	addition of 2 phr AC	1088:1107	The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites.
29727641	0	6	theme	sustainable	83:93	arg1	composites					107:116	sustainable wood starch composites	83:116	sustainable wood starch composites	83:116	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	1	7	theme	blending	303:310	arg1	technique					312:320	the solution blending technique	290:320	the solution blending technique using water as a solvent	290:345	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	2	8	theme	starch	446:451	arg1	MMA-g-starch					454:465	this MMA grafted starch (MMA-g-starch)	429:466	this MMA grafted starch (MMA-g-starch)	429:466	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	5	9	theme	outstanding	932:942	arg1	properties					955:964	outstanding mechanical properties	932:964	outstanding mechanical properties	932:964	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	2	10	theme	wood	505:508	arg1	flour					510:514	the cheap soft wood flour	490:514	the cheap soft wood flour	490:514	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	1	11	theme	natural	231:237	arg1	WSC					277:279	WSC	277:279	WSC	277:279	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	11	theme	natural	231:237	arg1	composites					265:274	natural polymer based wood starch composites	231:274	natural polymer based wood starch composites (WSC)	231:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	0	12	theme	starch	100:105	arg1	composites					107:116	sustainable wood starch composites	83:116	sustainable wood starch composites	83:116	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	5	13	theme	mechanical	944:953	arg1	properties					955:964	outstanding mechanical properties	932:964	outstanding mechanical properties	932:964	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	6	14	theme	2 phr	1100:1104	arg1	AC					1106:1107	2 phr AC	1100:1107	2 phr AC	1100:1107	The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites.
29727641	7	15	theme	significant	1165:1175	arg1	enhancement					1177:1187	A significant enhancement	1163:1187	A significant enhancement in water resistance properties of GO and AC treated composites	1163:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	0	16	theme	wood	95:98	arg1	composites					107:116	sustainable wood starch composites	83:116	sustainable wood starch composites	83:116	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	8	17	theme	curcas	1376:1381	arg1	seeds					1358:1362	the seeds	1354:1362	the seeds of Jatropha curcas	1354:1381	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	8	18	theme	Jatropha	1367:1374	arg1	curcas					1376:1381	Jatropha curcas	1367:1381	Jatropha curcas	1367:1381	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	2	19	theme	whole	584:588	arg1	process					590:596	the whole process	580:596	the whole process	580:596	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	4	20	theme	XRD	867:869	arg1	analysis					879:886	FTIR, XRD and SEM analysis	861:886	FTIR, XRD and SEM analysis	861:886	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	1	21	theme	Jatropha	155:162	arg1	curcas					164:169	Jatropha curcas	155:169	Jatropha curcas	155:169	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	0	22	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study on the properties of graphene oxide	0:54	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	0	23	theme	activated	60:68	arg1	carbon					70:75	activated carbon	60:75	activated carbon	60:75	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	6	24	theme	untreated	1141:1149	arg1	composites					1151:1160	untreated composites	1141:1160	untreated composites	1141:1160	The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites.
29727641	3	25	dep	Fourier	649:655	arg1	transform					657:665	transform	657:665	transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study	657:772	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	1	26	theme	polymer	239:245	arg1	WSC					277:279	WSC	277:279	WSC	277:279	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	26	theme	polymer	239:245	arg1	composites					265:274	natural polymer based wood starch composites	231:274	natural polymer based wood starch composites (WSC)	231:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	2	27	theme	citric	526:531	arg1	acid					533:536	the citric acid	522:536	the citric acid	522:536	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	2	27	theme	citric	526:531	arg1	solvent					569:575	a solvent	567:575	a solvent in the whole process	567:596	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	1	28	theme	based	247:251	arg1	WSC					277:279	WSC	277:279	WSC	277:279	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	28	theme	based	247:251	arg1	composites					265:274	natural polymer based wood starch composites	231:274	natural polymer based wood starch composites (WSC)	231:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	7	29	theme	water	1192:1196	arg1	properties					1209:1218	water resistance properties	1192:1218	water resistance properties of GO and AC treated composites	1192:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	7	30	theme	treated	1233:1239	arg1	composites					1241:1250	GO and AC treated composites	1223:1250	GO and AC treated composites	1223:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	4	31	with	interaction	779:789	arg1	wood					840:843	wood	840:843	wood	840:843	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	4	31	with	interaction	779:789	arg1	MMA-g-starch					810:821	MMA-g-starch	810:821	MMA-g-starch	810:821	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	4	31	with	interaction	779:789	arg1	acid					831:834	citric acid	824:834	citric acid	824:834	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	1	32	theme	graphene	175:182	arg1	oxide					184:188	graphene oxide	175:188	graphene oxide (GO)	175:193	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	4	33	theme	SEM	875:877	arg1	analysis					879:886	FTIR, XRD and SEM analysis	861:886	FTIR, XRD and SEM analysis	861:886	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	1	34	theme	wood	253:256	arg1	WSC					277:279	WSC	277:279	WSC	277:279	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	34	theme	wood	253:256	arg1	composites					265:274	natural polymer based wood starch composites	231:274	natural polymer based wood starch composites (WSC)	231:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	4	35	theme	GO	794:795	arg1	interaction					779:789	The interaction	775:789	The interaction	775:789	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	0	36	from	carbon	70:75	arg1	properties					27:36	the properties	23:36	the properties of graphene oxide	23:54	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	7	37	theme	composites	1241:1250	arg1	properties					1209:1218	water resistance properties	1192:1218	water resistance properties of GO and AC treated composites	1192:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	1	38	theme	starch	258:263	arg1	WSC					277:279	WSC	277:279	WSC	277:279	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	38	theme	starch	258:263	arg1	composites					265:274	natural polymer based wood starch composites	231:274	natural polymer based wood starch composites (WSC)	231:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	5	39	theme	treated	903:909	arg1	composites					911:920	treated composites	903:920	treated composites	903:920	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	3	40	dep	The	599:601	arg1	GO					612:613	GO	612:613	GO	612:613	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	1	41	theme	composites	265:274	arg1	preparation					216:226	the preparation	212:226	the preparation of natural polymer based wood starch composites (WSC)	212:280	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	4	42	theme	AC	801:802	arg1	interaction					779:789	The interaction	775:789	The interaction	775:789	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	7	43	theme	AC	1230:1231	arg1	composites					1241:1250	GO and AC treated composites	1223:1250	GO and AC treated composites	1223:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	2	44	theme	MMA	434:436	arg1	MMA-g-starch					454:465	this MMA grafted starch (MMA-g-starch)	429:466	this MMA grafted starch (MMA-g-starch)	429:466	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	0	45	theme	graphene	41:48	arg1	oxide					50:54	graphene oxide	41:54	graphene oxide	41:54	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	5	46	theme	resistance	994:1003	arg1	properties					1005:1014	fire resistance properties	989:1014	fire resistance properties	989:1014	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	2	47	theme	soft	500:503	arg1	flour					510:514	the cheap soft wood flour	490:514	the cheap soft wood flour	490:514	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	3	48	dep	transform	657:665	arg1	infrared					667:674	infrared	667:674	transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study	657:772	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	2	49	theme	cheap	494:498	arg1	flour					510:514	the cheap soft wood flour	490:514	the cheap soft wood flour	490:514	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	7	50	theme	GO	1223:1224	arg1	composites					1241:1250	GO and AC treated composites	1223:1250	GO and AC treated composites	1223:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	4	51	theme	FTIR	861:864	arg1	analysis					879:886	FTIR, XRD and SEM analysis	861:886	FTIR, XRD and SEM analysis	861:886	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
29727641	6	52	theme	composites	1045:1054	arg1	strength					1029:1036	The tensile strength	1017:1036	The tensile strength of the composites	1017:1054	The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites.
29727641	7	53	theme	resistance	1198:1207	arg1	properties					1209:1218	water resistance properties	1192:1218	water resistance properties of GO and AC treated composites	1192:1250	A significant enhancement in water resistance properties of GO and AC treated composites was also attained.
29727641	8	54	with	comparable	1393:1402	arg1	composites					1413:1422	the composites	1409:1422	the composites reinforced with GO	1409:1441	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	1	55	theme	Activated	119:127	arg1	AC					137:138	AC	137:138	AC	137:138	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	1	55	theme	Activated	119:127	arg1	carbon					129:134	Activated carbon	119:134	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO)	119:193	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	8	56	contain	containing	1326:1335	arg2	AC					1337:1338	AC	1337:1338	AC prepared from the seeds of Jatropha curcas	1337:1381	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	8	56	contain	containing	1326:1335	arg1	composites					1315:1324	the composites	1311:1324	the composites containing AC prepared from the seeds of Jatropha curcas	1311:1381	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	3	57	theme	X-ray	697:701	arg1	XRD					719:721	XRD	719:721	XRD	719:721	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	3	57	theme	X-ray	697:701	arg1	diffractometry					703:716	X-ray diffractometry	697:716	X-ray diffractometry (XRD)	697:722	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	6	58	theme	tensile	1021:1027	arg1	strength					1029:1036	The tensile strength	1017:1036	The tensile strength of the composites	1017:1054	The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites.
29727641	3	59	theme	thermogravimetric	725:741	arg1	TGA					753:755	TGA	753:755	TGA	753:755	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	3	59	theme	thermogravimetric	725:741	arg1	analysis					743:750	thermogravimetric analysis	725:750	thermogravimetric analysis (TGA)	725:756	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	2	60	theme	grafted	438:444	arg1	MMA-g-starch					454:465	this MMA grafted starch (MMA-g-starch)	429:466	this MMA grafted starch (MMA-g-starch)	429:466	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	8	61	theme	composites	1315:1324	arg1	comparable					1393:1402	comparable	1393:1402	comparable	1393:1402	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	8	61	theme	composites	1315:1324	arg1	properties					1297:1306	the properties	1293:1306	the properties of the composites containing AC prepared from the seeds of Jatropha curcas	1293:1381	The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO.
29727641	5	62	theme	fire	989:992	arg1	properties					1005:1014	fire resistance properties	989:1014	fire resistance properties	989:1014	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	2	63	theme	methyl	363:368	arg1	MMA					384:386	MMA	384:386	MMA	384:386	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	2	63	theme	methyl	363:368	arg1	methacrylate					370:381	methyl methacrylate	363:381	methyl methacrylate (MMA)	363:387	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	0	64	theme	oxide	50:54	arg1	properties					27:36	the properties	23:36	the properties of graphene oxide	23:54	A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.
29727641	5	65	theme	thermal	967:973	arg1	stability					975:983	thermal stability	967:983	thermal stability	967:983	The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties.
29727641	1	66	dep	oxide	184:188	arg1	GO					191:192	GO	191:192	GO	191:192	Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent.
29727641	2	67	from	solvent	569:575	arg1	process					590:596	the whole process	580:596	the whole process	580:596	In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process.
29727641	3	68	theme	Raman	762:766	arg1	study					768:772	Raman study	762:772	Raman study	762:772	The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study.
29727641	4	69	theme	citric	824:829	arg1	acid					831:834	citric acid	824:834	citric acid	824:834	The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis.
31381916	0	0	theme	cornstarch-based	71:86	arg1	biocomposites					88:100	cornstarch-based biocomposites	71:100	cornstarch-based biocomposites	71:100	Potential of using multiscale corn husk fiber as reinforcing filler in cornstarch-based biocomposites.
31381916	7	1	theme	reinforced	1172:1181	arg1	films					1183:1187	the reinforced films	1168:1187	the reinforced films	1168:1187	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	3	2	theme	thermal	451:457	arg1	characteristics					487:501	The Physical, thermal, morphological, and tensile characteristics	437:501	The Physical, thermal, morphological, and tensile characteristics of composite films	437:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	6	3	theme	tensile	967:973	arg1	strength					975:982	the improved tensile strength	954:982	the improved tensile strength	954:982	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	5	4	theme	moisture	715:722	arg1	content					724:730	moisture content	715:730	moisture content	715:730	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	7	5	theme	thermal	1042:1048	arg1	stability					1050:1058	The thermal stability	1038:1058	The thermal stability of composite films	1038:1077	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	8	6	theme	fiber	1313:1317	arg1	loading					1319:1325	fiber loading	1313:1325	fiber loading	1313:1325	Fourier transform infrared analysis revealed increasing in intermolecular hydrogen bonding following fiber loading.
31381916	6	7	theme	improved	958:965	arg1	strength					975:982	the improved tensile strength	954:982	the improved tensile strength	954:982	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	6	8	theme	consistent	852:861	arg1	structure					863:871	consistent structure	852:871	consistent structure	852:871	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	7	9	theme	films	1073:1077	arg1	stability					1050:1058	The thermal stability	1038:1058	The thermal stability of composite films	1038:1077	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	3	10	theme	films	516:520	arg1	characteristics					487:501	The Physical, thermal, morphological, and tensile characteristics	437:501	The Physical, thermal, morphological, and tensile characteristics of composite films	437:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	5	11	theme	noticeable	675:684	arg1	reduction					686:694	a noticeable reduction	673:694	a noticeable reduction in the density and moisture content of the films	673:743	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	4	12	theme	composite	646:654	arg1	films					656:660	the composite films	642:660	the composite films	642:660	The findings indicated that the incorporation of husk fiber, in general, enhanced the performance of the composite films.
31381916	8	13	theme	intermolecular	1271:1284	arg1	bonding					1295:1301	intermolecular hydrogen bonding	1271:1301	intermolecular hydrogen bonding following fiber loading	1271:1325	Fourier transform infrared analysis revealed increasing in intermolecular hydrogen bonding following fiber loading.
31381916	7	14	theme	decomposition	1139:1151	arg1	temperature					1153:1163	the increased onset decomposition temperature	1119:1163	the increased onset decomposition temperature of the reinforced films compared to neat film	1119:1209	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	3	15	theme	Physical	441:448	arg1	characteristics					487:501	The Physical, thermal, morphological, and tensile characteristics	437:501	The Physical, thermal, morphological, and tensile characteristics of composite films	437:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	4	16	theme	husk	590:593	arg1	fiber					595:599	husk fiber	590:599	husk fiber	590:599	The findings indicated that the incorporation of husk fiber, in general, enhanced the performance of the composite films.
31381916	9	17	theme	polymeric	1454:1462	arg1	disposal					1470:1477	agricultural and polymeric waste disposal	1437:1477	agricultural and polymeric waste disposal	1437:1477	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	9	18	theme	agricultural	1437:1448	arg1	disposal					1470:1477	agricultural and polymeric waste disposal	1437:1477	agricultural and polymeric waste disposal	1437:1477	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	5	19	theme	films	739:743	arg1	density					703:709	density	703:709	density	703:709	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	5	19	theme	films	739:743	arg1	content					724:730	moisture content	715:730	moisture content	715:730	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	6	20	dep	structure	863:871	arg1	a					850:850	a	850:850	a	850:850	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	3	21	theme	tensile	479:485	arg1	characteristics					487:501	The Physical, thermal, morphological, and tensile characteristics	437:501	The Physical, thermal, morphological, and tensile characteristics of composite films	437:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	1	22	theme	thermoplastic	171:183	arg1	matrix					196:201	thermoplastic cornstarch matrix	171:201	thermoplastic cornstarch matrix	171:201	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	22	theme	thermoplastic	171:183	arg1	filler					240:245	a reinforcing filler	226:245	a reinforcing filler	226:245	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	22	theme	thermoplastic	171:183	arg1	fiber					217:221	corn husk fiber	207:221	corn husk fiber	207:221	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	7	23	theme	increased	1123:1131	arg1	temperature					1153:1163	the increased onset decomposition temperature	1119:1163	the increased onset decomposition temperature of the reinforced films compared to neat film	1119:1209	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	6	24	theme	morphological	819:831	arg1	images					833:838	The morphological images	815:838	The morphological images	815:838	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	7	25	theme	films	1183:1187	arg1	temperature					1153:1163	the increased onset decomposition temperature	1119:1163	the increased onset decomposition temperature of the reinforced films compared to neat film	1119:1209	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	2	26	theme	different	316:324	arg1	plasticizer					381:391	a plasticizer	379:391	a plasticizer at a fixed amount of 25% for starch weight	379:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	26	theme	different	316:324	arg1	concentrations					326:339	different concentrations	316:339	different concentrations of husk fiber (0-8%), and fructose	316:374	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	1	27	theme	cornstarch	185:194	arg1	matrix					196:201	thermoplastic cornstarch matrix	171:201	thermoplastic cornstarch matrix	171:201	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	27	theme	cornstarch	185:194	arg1	filler					240:245	a reinforcing filler	226:245	a reinforcing filler	226:245	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	27	theme	cornstarch	185:194	arg1	fiber					217:221	corn husk fiber	207:221	corn husk fiber	207:221	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	7	28	theme	onset	1133:1137	arg1	temperature					1153:1163	the increased onset decomposition temperature	1119:1163	the increased onset decomposition temperature of the reinforced films compared to neat film	1119:1209	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	6	29	theme	crystallinity	1017:1029	arg1	index					1031:1035	the crystallinity index	1013:1035	the crystallinity index	1013:1035	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	0	30	theme	multiscale	19:28	arg1	fiber					40:44	multiscale corn husk fiber	19:44	multiscale corn husk fiber	19:44	Potential of using multiscale corn husk fiber as reinforcing filler in cornstarch-based biocomposites.
31381916	8	31	theme	hydrogen	1286:1293	arg1	bonding					1295:1301	intermolecular hydrogen bonding	1271:1301	intermolecular hydrogen bonding following fiber loading	1271:1325	Fourier transform infrared analysis revealed increasing in intermolecular hydrogen bonding following fiber loading.
31381916	3	32	theme	composite	506:514	arg1	films					516:520	composite films	506:520	composite films	506:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	4	33	theme	fiber	595:599	arg1	incorporation					573:585	the incorporation	569:585	the incorporation of husk fiber	569:599	The findings indicated that the incorporation of husk fiber, in general, enhanced the performance of the composite films.
31381916	2	34	theme	fixed	398:402	arg1	amount					404:409	a fixed amount	396:409	a fixed amount of 25% for starch weight	396:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	34	theme	fixed	398:402	arg1	%					416:416	25%	414:416	25% for starch weight	414:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	9	35	theme	composite	1332:1340	arg1	materials					1342:1350	The composite materials	1328:1350	The composite materials prepared using corn husk residues as reinforcement	1328:1401	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	9	36	theme	waste	1503:1507	arg1	management					1509:1518	waste management	1503:1518	waste management	1503:1518	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	5	37	from	reduction	686:694	arg1	density					703:709	density	703:709	density	703:709	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	5	37	from	reduction	686:694	arg1	content					724:730	moisture content	715:730	moisture content	715:730	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	2	38	theme	casting	292:298	arg1	technique					300:308	a casting technique	290:308	a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight	290:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	1	39	theme	corn	207:210	arg1	filler					240:245	a reinforcing filler	226:245	a reinforcing filler	226:245	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	39	theme	corn	207:210	arg1	matrix					196:201	thermoplastic cornstarch matrix	171:201	thermoplastic cornstarch matrix	171:201	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	39	theme	corn	207:210	arg1	fiber					217:221	corn husk fiber	207:221	corn husk fiber	207:221	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	0	40	theme	husk	35:38	arg1	fiber					40:44	multiscale corn husk fiber	19:44	multiscale corn husk fiber	19:44	Potential of using multiscale corn husk fiber as reinforcing filler in cornstarch-based biocomposites.
31381916	4	41	theme	films	656:660	arg1	performance					627:637	the performance	623:637	the performance of the composite films	623:660	The findings indicated that the incorporation of husk fiber, in general, enhanced the performance of the composite films.
31381916	9	42	theme	waste	1464:1468	arg1	disposal					1470:1477	agricultural and polymeric waste disposal	1437:1477	agricultural and polymeric waste disposal	1437:1477	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	1	43	theme	husk	212:215	arg1	filler					240:245	a reinforcing filler	226:245	a reinforcing filler	226:245	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	43	theme	husk	212:215	arg1	matrix					196:201	thermoplastic cornstarch matrix	171:201	thermoplastic cornstarch matrix	171:201	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	43	theme	husk	212:215	arg1	fiber					217:221	corn husk fiber	207:221	corn husk fiber	207:221	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	2	44	theme	starch	422:427	arg1	weight					429:434	starch weight	422:434	starch weight	422:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	0	45	theme	corn	30:33	arg1	fiber					40:44	multiscale corn husk fiber	19:44	multiscale corn husk fiber	19:44	Potential of using multiscale corn husk fiber as reinforcing filler in cornstarch-based biocomposites.
31381916	0	46	from	filler	61:66	arg1	biocomposites					88:100	cornstarch-based biocomposites	71:100	cornstarch-based biocomposites	71:100	Potential of using multiscale corn husk fiber as reinforcing filler in cornstarch-based biocomposites.
31381916	5	47	theme	soil	750:753	arg1	assessment					762:771	soil burial assessment	750:771	soil burial assessment	750:771	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	8	48	dep	Fourier	1212:1218	arg1	transform					1220:1228	transform	1220:1228	transform infrared analysis	1220:1246	Fourier transform infrared analysis revealed increasing in intermolecular hydrogen bonding following fiber loading.
31381916	2	49	theme	fiber	349:353	arg1	plasticizer					381:391	a plasticizer	379:391	a plasticizer at a fixed amount of 25% for starch weight	379:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	49	theme	fiber	349:353	arg1	concentrations					326:339	different concentrations	316:339	different concentrations of husk fiber (0-8%), and fructose	316:374	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	50	theme	%	416:416	arg1	amount					404:409	a fixed amount	396:409	a fixed amount of 25% for starch weight	396:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	50	theme	%	416:416	arg1	%					416:416	25%	414:416	25% for starch weight	414:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	9	51	theme	corn	1367:1370	arg1	residues					1377:1384	corn husk residues	1367:1384	corn husk residues	1367:1384	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	6	52	theme	excellent	877:885	arg1	compatibility					887:899	excellent compatibility	877:899	excellent compatibility	877:899	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	7	53	theme	composite	1063:1071	arg1	films					1073:1077	composite films	1063:1077	composite films	1063:1077	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31381916	2	54	theme	husk	344:347	arg1	fiber					349:353	husk fiber	344:353	husk fiber (0-8%)	344:360	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	54	theme	husk	344:347	arg1	%					359:359	0-8%	356:359	0-8%	356:359	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	5	55	dep	density	703:709	arg1	the					699:701	the	699:701	the	699:701	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	9	56	theme	husk	1372:1375	arg1	residues					1377:1384	corn husk residues	1367:1384	corn husk residues	1367:1384	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	1	57	theme	biodegradable	118:130	arg1	films					142:146	biodegradable composite films	118:146	biodegradable composite films	118:146	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	8	58	dep	transform	1220:1228	arg1	infrared					1230:1237	infrared	1230:1237	transform infrared analysis	1220:1246	Fourier transform infrared analysis revealed increasing in intermolecular hydrogen bonding following fiber loading.
31381916	9	59	theme	community	1416:1424	arg1	demand					1426:1431	community demand	1416:1431	community demand for agricultural and polymeric waste disposal	1416:1477	The composite materials prepared using corn husk residues as reinforcement responded to community demand for agricultural and polymeric waste disposal and added more value to waste management.
31381916	2	60	theme	composite	252:260	arg1	films					262:266	The composite films	248:266	The composite films	248:266	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	1	61	theme	composite	132:140	arg1	films					142:146	biodegradable composite films	118:146	biodegradable composite films	118:146	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	6	62	theme	young	988:992	arg1	modulus					994:1000	young modulus	988:1000	young modulus	988:1000	The morphological images presented a consistent structure and excellent compatibility between matrix and reinforcement, which reflected on the improved tensile strength and young modulus as well as the crystallinity index.
31381916	3	63	theme	morphological	460:472	arg1	characteristics					487:501	The Physical, thermal, morphological, and tensile characteristics	437:501	The Physical, thermal, morphological, and tensile characteristics of composite films	437:520	The Physical, thermal, morphological, and tensile characteristics of composite films were investigated.
31381916	2	64	theme	fructose	367:374	arg1	plasticizer					381:391	a plasticizer	379:391	a plasticizer at a fixed amount of 25% for starch weight	379:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	64	theme	fructose	367:374	arg1	concentrations					326:339	different concentrations	316:339	different concentrations of husk fiber (0-8%), and fructose	316:374	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	1	65	theme	reinforcing	228:238	arg1	filler					240:245	a reinforcing filler	226:245	a reinforcing filler	226:245	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	65	theme	reinforcing	228:238	arg1	matrix					196:201	thermoplastic cornstarch matrix	171:201	thermoplastic cornstarch matrix	171:201	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	1	65	theme	reinforcing	228:238	arg1	fiber					217:221	corn husk fiber	207:221	corn husk fiber	207:221	In this study, biodegradable composite films were prepared by using thermoplastic cornstarch matrix and corn husk fiber as a reinforcing filler.
31381916	2	66	from	amount	404:409	arg1	plasticizer					381:391	a plasticizer	379:391	a plasticizer at a fixed amount of 25% for starch weight	379:434	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	2	66	from	amount	404:409	arg1	concentrations					326:339	different concentrations	316:339	different concentrations of husk fiber (0-8%), and fructose	316:374	The composite films were manufactured via a casting technique using different concentrations of husk fiber (0-8%), and fructose as a plasticizer at a fixed amount of 25% for starch weight.
31381916	5	67	theme	burial	755:760	arg1	assessment					762:771	soil burial assessment	750:771	soil burial assessment	750:771	There was a noticeable reduction in the density and moisture content of the films, and soil burial assessment showed less resistance to biodegradation.
31381916	7	68	theme	neat	1201:1204	arg1	film					1206:1209	neat film	1201:1209	neat film	1201:1209	The thermal stability of composite films has also been enhanced, as evidenced by the increased onset decomposition temperature of the reinforced films compared to neat film.
31847112	5	0	theme	ethylene	753:760	arg1	poly					741:744	poly	741:744	poly(methyl ethylene phosphate) (PMEP)	741:778	PNIPAm is co-polymerized with poly(methyl ethylene phosphate) (PMEP), a poloyphosphoester.
31847112	5	0	theme	ethylene	753:760	arg1	phosphate					762:770	methyl ethylene phosphate	746:770	methyl ethylene phosphate	746:770	PNIPAm is co-polymerized with poly(methyl ethylene phosphate) (PMEP), a poloyphosphoester.
31847112	6	1	used	used	949:952	arg2	brush					940:944	pure PNIPAm brush	928:944	pure PNIPAm brush	928:944	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	6	1	used	used	949:952	arg2	reference					957:965	reference	957:965	reference	957:965	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	9	2	theme	debonding	1335:1343	arg1	rate					1345:1348	debonding rate	1335:1348	debonding rate	1335:1348	Secondly, effect of adhesion parameters such as debonding rate and contact time is studied.
31847112	1	3	theme	wet	157:159	arg1	adhesives					161:169	wet adhesives	157:169	wet adhesives	157:169	During the last few decades, wet adhesives have been developed for applications in various fields.
31847112	3	4	from	approach	536:543	arg1	surfaces					553:560	flat surfaces	548:560	flat surfaces	548:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	9	5	theme	contact	1354:1360	arg1	time					1362:1365	contact time	1354:1365	contact time	1354:1365	Secondly, effect of adhesion parameters such as debonding rate and contact time is studied.
31847112	0	6	theme	Molecular	79:87	arg1	Architecture					89:100	Molecular Architecture	79:100	Molecular Architecture	79:100	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	11	7	theme	hydrophilic	1569:1579	arg1	substrates					1597:1606	hydrophilic and hydrophobic substrates	1569:1606	hydrophilic and hydrophobic substrates	1569:1606	Finally, the adhesive properties of the layers are studied below and above the LCST against hydrophilic and hydrophobic substrates.
31847112	2	8	theme	suitable	321:328	arg1	composition					339:349	the most suitable chemical composition	312:349	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	0	9	theme	Underwater	106:115	arg1	Adherence					117:125	Underwater Adherence	106:125	Underwater Adherence	106:125	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	11	10	theme	adhesive	1490:1497	arg1	properties					1499:1508	the adhesive properties	1486:1508	the adhesive properties of the layers	1486:1522	Finally, the adhesive properties of the layers are studied below and above the LCST against hydrophilic and hydrophobic substrates.
31847112	3	11	theme	flat	548:551	arg1	surfaces					553:560	flat surfaces	548:560	flat surfaces	548:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	2	12	theme	polymer	280:286	arg1	architecture					288:299	the most suitable polymer architecture	262:299	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	3	13	theme	chain	483:487	arg1	architecture					489:500	side graft chain architecture	472:500	side graft chain architecture prepared using "grafting through" approach on flat surfaces	472:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	10	14	theme	adhesive	1413:1420	arg1	properties					1422:1431	the adhesive properties	1409:1431	the adhesive properties	1409:1431	Thirdly, the reversibility of the adhesive properties is confirmed by performing adhesion cycles.
31847112	4	15	from	incorporation	567:579	arg1	backbone					588:595	the backbone	584:595	the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm)	584:653	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	2	16	theme	suitable	271:278	arg1	architecture					288:299	the most suitable polymer architecture	262:299	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	3	17	theme	novel	434:438	arg1	brushes					459:465	novel responsive polymer brushes	434:465	novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	434:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	0	18	theme	Graft	44:48	arg1	Chains					50:55	Side Graft Chains	39:55	Side Graft Chains	39:55	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	3	19	with	brushes	459:465	arg1	architecture					489:500	side graft chain architecture	472:500	side graft chain architecture prepared using "grafting through" approach on flat surfaces	472:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	4	20	theme	thermo-responsive	600:616	arg1	PNIPAm					647:652	PNIPAm	647:652	PNIPAm	647:652	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	4	20	theme	thermo-responsive	600:616	arg1	poly					618:621	thermo-responsive poly	600:621	thermo-responsive poly(N-isopropylacrylamide) (PNIPAm)	600:653	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	4	20	theme	thermo-responsive	600:616	arg1	N-isopropylacrylamide					623:643	N-isopropylacrylamide	623:643	N-isopropylacrylamide	623:643	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	3	21	theme	responsive	440:449	arg1	brushes					459:465	novel responsive polymer brushes	434:465	novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	434:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	0	22	theme	Polymer	18:24	arg1	Brushes					26:32	Thermo-Responsive Polymer Brushes	0:32	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.	0:126	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	6	23	theme	pure	928:931	arg1	reference					957:965	reference	957:965	reference	957:965	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	6	23	theme	pure	928:931	arg1	brush					940:944	pure PNIPAm brush	928:944	pure PNIPAm brush	928:944	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	11	24	theme	layers	1517:1522	arg1	properties					1499:1508	the adhesive properties	1486:1508	the adhesive properties of the layers	1486:1522	Finally, the adhesive properties of the layers are studied below and above the LCST against hydrophilic and hydrophobic substrates.
31847112	8	25	dep	show	1156:1159	arg1	increased					1185:1193	increased	1185:1193	increased hydrophobicity and variation of the surface charge even if no ionizable groups are present	1185:1284	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	0	26	theme	Thermo-Responsive	0:16	arg1	Brushes					26:32	Thermo-Responsive Polymer Brushes	0:32	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.	0:126	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	7	27	theme	PNIPAm-g-PMEP	968:980	arg1	copolymers					982:991	PNIPAm-g-PMEP copolymers	968:991	PNIPAm-g-PMEP copolymers	968:991	PNIPAm-g-PMEP copolymers retain the responsive behavior of PNIPAm: when T > LCST, a clear switching of properties is observed.
31847112	7	28	theme	properties	1071:1080	arg1	switching					1058:1066	a clear switching	1050:1066	a clear switching of properties	1050:1080	PNIPAm-g-PMEP copolymers retain the responsive behavior of PNIPAm: when T > LCST, a clear switching of properties is observed.
31847112	6	29	theme	PNIPAm	933:938	arg1	reference					957:965	reference	957:965	reference	957:965	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	6	29	theme	PNIPAm	933:938	arg1	brush					940:944	pure PNIPAm brush	928:944	pure PNIPAm brush	928:944	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	3	30	theme	"	534:534	arg1	approach					536:543	" approach	534:543	" approach on flat surfaces	534:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	6	31	theme	surface-grafted	853:867	arg1	polymer					869:875	the surface-grafted polymer	849:875	the surface-grafted polymer as well as the polymer from the bulk solution	849:921	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	3	32	theme	side	472:475	arg1	architecture					489:500	side graft chain architecture	472:500	side graft chain architecture prepared using "grafting through" approach on flat surfaces	472:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	3	33	theme	underwater	400:409	arg1	properties					420:429	the underwater adhesion properties	396:429	the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	396:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	4	34	theme	final	697:701	arg1	layers					703:708	the final layers	693:708	the final layers	693:708	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	1	35	theme	various	211:217	arg1	fields					219:224	various fields	211:224	various fields	211:224	During the last few decades, wet adhesives have been developed for applications in various fields.
31847112	2	36	theme	key	240:242	arg1	architecture					288:299	the most suitable polymer architecture	262:299	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	2	36	theme	key	240:242	arg1	composition					339:349	the most suitable chemical composition	312:349	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	2	36	theme	key	240:242	arg1	questions					244:252	key questions	240:252	key questions such as the most suitable polymer architecture as well as the most suitable chemical composition	240:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	3	37	theme	polymer	451:457	arg1	brushes					459:465	novel responsive polymer brushes	434:465	novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	434:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	0	38	theme	Side	39:42	arg1	Chains					50:55	Side Graft Chains	39:55	Side Graft Chains	39:55	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	3	39	theme	adhesion	411:418	arg1	properties					420:429	the underwater adhesion properties	396:429	the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	396:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	8	40	theme	surface	1231:1237	arg1	charge					1239:1244	the surface charge	1227:1244	the surface charge	1227:1244	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	3	41	theme	brushes	459:465	arg1	properties					420:429	the underwater adhesion properties	396:429	the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces	396:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	8	42	theme	chains	1177:1182	arg1	collapse					1161:1168	collapse	1161:1168	collapse of the chains	1161:1182	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	9	43	theme	adhesion	1307:1314	arg1	parameters					1316:1325	adhesion parameters	1307:1325	adhesion parameters	1307:1325	Secondly, effect of adhesion parameters such as debonding rate and contact time is studied.
31847112	6	44	from	solution	914:921	arg1	polymer					892:898	the polymer	888:898	the surface-grafted polymer as well as the polymer from the bulk solution	849:921	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	6	44	from	solution	914:921	arg1	polymer					869:875	the surface-grafted polymer	849:875	the surface-grafted polymer as well as the polymer from the bulk solution	849:921	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	0	45	with	Brushes	26:32	arg1	Chains					50:55	Side Graft Chains	39:55	Side Graft Chains	39:55	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	8	46	theme	ionizable	1257:1265	arg1	groups					1267:1272	no ionizable groups	1254:1272	no ionizable groups	1254:1272	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	10	47	theme	properties	1422:1431	arg1	reversibility					1392:1404	the reversibility	1388:1404	the reversibility of the adhesive properties	1388:1431	Thirdly, the reversibility of the adhesive properties is confirmed by performing adhesion cycles.
31847112	9	48	theme	parameters	1316:1325	arg1	effect					1297:1302	effect	1297:1302	effect of adhesion parameters such as debonding rate and contact time	1297:1365	Secondly, effect of adhesion parameters such as debonding rate and contact time is studied.
31847112	7	49	theme	PNIPAm	1027:1032	arg1	behavior					1015:1022	the responsive behavior	1000:1022	the responsive behavior of PNIPAm	1000:1032	PNIPAm-g-PMEP copolymers retain the responsive behavior of PNIPAm: when T > LCST, a clear switching of properties is observed.
31847112	7	50	theme	clear	1052:1056	arg1	switching					1058:1066	a clear switching	1050:1066	a clear switching of properties	1050:1080	PNIPAm-g-PMEP copolymers retain the responsive behavior of PNIPAm: when T > LCST, a clear switching of properties is observed.
31847112	1	51	from	applications	195:206	arg1	fields					219:224	various fields	211:224	various fields	211:224	During the last few decades, wet adhesives have been developed for applications in various fields.
31847112	2	52	theme	chemical	330:337	arg1	composition					339:349	the most suitable chemical composition	312:349	the most suitable polymer architecture as well as the most suitable chemical composition	262:349	Nonetheless, key questions such as the most suitable polymer architecture as well as the most suitable chemical composition remain open.
31847112	6	53	theme	bulk	909:912	arg1	solution					914:921	the bulk solution	905:921	the bulk solution	905:921	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	7	54	theme	responsive	1004:1013	arg1	behavior					1015:1022	the responsive behavior	1000:1022	the responsive behavior of PNIPAm	1000:1032	PNIPAm-g-PMEP copolymers retain the responsive behavior of PNIPAm: when T > LCST, a clear switching of properties is observed.
31847112	8	55	theme	critical	1135:1142	arg1	temperature					1144:1154	the critical temperature	1131:1154	the critical temperature	1131:1154	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	1	56	theme	last	139:142	arg1	decades					148:154	the last few decades	135:154	the last few decades	135:154	During the last few decades, wet adhesives have been developed for applications in various fields.
31847112	4	57	theme	poly	618:621	arg1	backbone					588:595	the backbone	584:595	the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm)	584:653	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	6	58	theme	final	806:810	arg1	materials					812:820	The final materials	802:820	The final materials	802:820	The final materials are characterized studying the surface-grafted polymer as well as the polymer from the bulk solution, and pure PNIPAm brush is used as reference.
31847112	0	59	dep	Brushes	26:32	arg1	Relationship					58:69	Relationship	58:69	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.	0:126	Thermo-Responsive Polymer Brushes with Side Graft Chains: Relationship Between Molecular Architecture and Underwater Adherence.
31847112	1	60	theme	few	144:146	arg1	decades					148:154	the last few decades	135:154	the last few decades	135:154	During the last few decades, wet adhesives have been developed for applications in various fields.
31847112	3	61	theme	graft	477:481	arg1	architecture					489:500	side graft chain architecture	472:500	side graft chain architecture prepared using "grafting through" approach on flat surfaces	472:560	In this article, we investigate the underwater adhesion properties of novel responsive polymer brushes with side graft chain architecture prepared using "grafting through" approach on flat surfaces.
31847112	10	62	theme	adhesion	1460:1467	arg1	cycles					1469:1474	adhesion cycles	1460:1474	adhesion cycles	1460:1474	Thirdly, the reversibility of the adhesive properties is confirmed by performing adhesion cycles.
31847112	5	63	theme	methyl	746:751	arg1	poly					741:744	poly	741:744	poly(methyl ethylene phosphate) (PMEP)	741:778	PNIPAm is co-polymerized with poly(methyl ethylene phosphate) (PMEP), a poloyphosphoester.
31847112	5	63	theme	methyl	746:751	arg1	phosphate					762:770	methyl ethylene phosphate	746:770	methyl ethylene phosphate	746:770	PNIPAm is co-polymerized with poly(methyl ethylene phosphate) (PMEP), a poloyphosphoester.
31847112	11	64	theme	hydrophobic	1585:1595	arg1	substrates					1597:1606	hydrophilic and hydrophobic substrates	1569:1606	hydrophilic and hydrophobic substrates	1569:1606	Finally, the adhesive properties of the layers are studied below and above the LCST against hydrophilic and hydrophobic substrates.
31847112	4	65	theme	LCST	676:679	arg1	behavior					681:688	LCST behavior	676:688	LCST behavior	676:688	The incorporation in the backbone of thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) allowed us to obtain LCST behavior in the final layers.
31847112	8	66	theme	charge	1239:1244	arg1	hydrophobicity					1195:1208	hydrophobicity	1195:1208	hydrophobicity	1195:1208	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
31847112	8	66	theme	charge	1239:1244	arg1	variation					1214:1222	variation	1214:1222	variation	1214:1222	More specifically, all layers above the critical temperature show collapse of the chains, increased hydrophobicity and variation of the surface charge even if no ionizable groups are present.
29895407	0	0	theme	chromatography	195:208	arg1	mode					210:213	liquid and subcritical fluid chromatography mode	166:213	liquid and subcritical fluid chromatography mode	166:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	1	theme	analogs	469:475	arg1	separation					378:387	the enantiomer separation	363:387	the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	363:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	1	2	theme	supercritical	271:283	arg1	fluid					285:289	supercritical fluid	271:289	supercritical fluid	271:289	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	7	3	theme	mobile	1759:1764	arg1	phases					1766:1771	the mobile phases	1755:1771	the mobile phases	1755:1771	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	8	4	located	observed	1946:1953	arg1	mode					1869:1872	SFC mode	1865:1872	SFC mode	1865:1872	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	8	4	located	observed	1946:1953	arg2	separations					1929:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	8	4	located	observed	1946:1953	arg1	columns					1807:1813	columns	1807:1813	columns operated in NP-LC mode enthalpically-	1807:1851	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	8	4	located	observed	1946:1953	arg1	CSPs					1798:1801	polysaccharide-based CSPs	1777:1801	polysaccharide-based CSPs	1777:1801	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	5	5	theme	LC	1424:1425	arg1	modes					1435:1439	both LC and SFC modes	1419:1439	both LC and SFC modes	1419:1439	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	1	6	theme	chromatographic	291:305	arg1	methods					331:337	chromatographic (both acronymed as SFC) methods	291:337	chromatographic (both acronymed as SFC) methods	291:337	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	2	7	theme	retention	782:790	arg1	characteristics					792:806	retention characteristics	782:806	retention characteristics	782:806	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	8	8	theme	entropically-driven	1898:1916	arg1	separations					1929:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	7	9	theme	analytes	1724:1731	arg1	selectors					1696:1704	selectors	1696:1704	selectors	1696:1704	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	7	9	theme	analytes	1724:1731	arg1	properties					1741:1750	the properties	1737:1750	the properties of the mobile phases	1737:1771	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	7	9	theme	analytes	1724:1731	arg1	structure					1711:1719	the structure	1707:1719	the structure of analytes	1707:1731	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	0	10	theme	ampholytic	137:146	arg1	analogs					155:161	basic and ampholytic indole analogs	127:161	basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode	127:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	11	theme	basic	398:402	arg1	separation					378:387	the enantiomer separation	363:387	the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	363:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	4	12	theme	amine	1164:1168	arg1	additives					1170:1178	amine additives	1164:1178	amine additives	1164:1178	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	8	13	theme	enthalpically-	1879:1892	arg1	separations					1929:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	1	14	dep	liquid	251:256	arg1	methods					331:337	chromatographic (both acronymed as SFC) methods	291:337	chromatographic (both acronymed as SFC) methods	291:337	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	0	15	theme	liquid	166:171	arg1	chromatography					195:208	liquid and subcritical fluid chromatography	166:208	chromatography	195:208	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	6	16	theme	free	1581:1584	arg1	ΔG°					1595:1597	ΔG°	1595:1597	ΔG°	1595:1597	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	16	theme	free	1581:1584	arg1	Δ					1593:1593	free energy Δ	1581:1593	free energy Δ(ΔG°)	1581:1598	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	1	17	dep	chromatographic	291:305	arg1	both					308:311	both	308:311	both	308:311	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	0	18	theme	subcritical	177:187	arg1	chromatography					195:208	liquid and subcritical fluid chromatography	166:208	chromatography	195:208	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	19	theme	C-3-substituted	446:460	arg1	analogs					469:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	3	20	theme	polysaccharide-based	891:910	arg1	CSPs					912:915	all studied polysaccharide-based CSPs	879:915	all studied polysaccharide-based CSPs	879:915	On all studied polysaccharide-based CSPs the three ampholytes remained unretained in NP-LC mode, while they were nicely retained and resolved in SFC mode.
29895407	2	21	theme	amine	759:763	arg1	additives					765:773	amine additives	759:773	amine additives	759:773	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	5	22	theme	basic	1353:1357	arg1	analytes					1374:1381	the basic and ampholytic analytes	1349:1381	the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes	1349:1439	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	4	23	theme	unexpected	1037:1046	arg1	results					1048:1054	These unexpected results	1031:1054	These unexpected results	1031:1054	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	2	24	theme	alcohol	747:753	arg1	composition					696:706	the composition	692:706	the composition of the mobile phase	692:726	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	24	theme	alcohol	747:753	arg1	nature					733:738	the nature	729:738	the nature of the alcohol	729:753	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	24	theme	alcohol	747:753	arg1	additives					765:773	amine additives	759:773	amine additives	759:773	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	0	25	theme	stationary	97:106	arg1	phases					108:113	chiral stationary phases	90:113	chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode	90:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	2	26	theme	composition	696:706	arg1	effect					682:687	the effect	678:687	the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes	678:855	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	1	27	theme	enantiomer	367:376	arg1	separation					378:387	the enantiomer separation	363:387	the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	363:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	4	28	with	combination	1119:1129	arg1	alcohols					1136:1143	alcohols	1136:1143	alcohols as co-solvents and amine additives	1136:1178	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	8	29	theme	polysaccharide-based	1777:1796	arg1	CSPs					1798:1801	polysaccharide-based CSPs	1777:1801	polysaccharide-based CSPs	1777:1801	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	7	30	theme	parameters	1659:1668	arg1	values					1631:1636	The values	1627:1636	The values of the thermodynamic parameters	1627:1668	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	5	31	theme	polar	1391:1395	arg1	phase					1410:1414	a polar ionic mobile phase	1389:1414	a polar ionic mobile phase	1389:1414	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	0	32	theme	basic	127:131	arg1	analogs					155:161	basic and ampholytic indole analogs	127:161	basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode	127:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	3	33	theme	NP-LC	961:965	arg1	mode					967:970	NP-LC mode	961:970	NP-LC mode	961:970	On all studied polysaccharide-based CSPs the three ampholytes remained unretained in NP-LC mode, while they were nicely retained and resolved in SFC mode.
29895407	2	34	theme	phase	722:726	arg1	composition					696:706	the composition	692:706	the composition of the mobile phase	692:726	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	34	theme	phase	722:726	arg1	nature					733:738	the nature	729:738	the nature of the alcohol	729:753	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	34	theme	phase	722:726	arg1	additives					765:773	amine additives	759:773	amine additives	759:773	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	5	35	theme	mobile	1403:1408	arg1	phase					1410:1414	a polar ionic mobile phase	1389:1414	a polar ionic mobile phase	1389:1414	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	0	36	theme	Dedicated	0:8	arg1	comparisons					10:20	Dedicated comparisons	0:20	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona	0:73	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	4	37	theme	CO2	1096:1098	arg1	property					1077:1084	a specific property	1066:1084	a specific property of liquid CO2	1066:1098	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	4	38	theme	ampholytes	1273:1282	arg1	retention					1260:1268	the retention	1256:1268	the retention of ampholytes	1256:1282	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	5	39	theme	ampholytic	1363:1372	arg1	analytes					1374:1381	the basic and ampholytic analytes	1349:1381	the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes	1349:1439	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	1	40	from	separation	378:387	arg1	phases					639:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases	531:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	531:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	1	40	from	separation	378:387	arg1	CSPs					647:650	CSPs	647:650	CSPs	647:650	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	1	40	from	separation	378:387	arg1	polysaccharide-based					506:525	polysaccharide-based	506:525	polysaccharide-based	506:525	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	0	41	theme	polysaccharide-	33:47	arg1	comparisons					10:20	Dedicated comparisons	0:20	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona	0:73	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	4	42	theme	specific	1068:1075	arg1	property					1077:1084	a specific property	1066:1084	a specific property of liquid CO2	1066:1098	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	5	43	theme	SFC	1431:1433	arg1	modes					1435:1439	both LC and SFC modes	1419:1439	both LC and SFC modes	1419:1439	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	0	44	theme	zwitterionic	53:64	arg1	Cinchona					66:73	zwitterionic Cinchona	53:73	zwitterionic Cinchona	53:73	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	45	from	polysaccharide-based	506:525	arg1	separation					378:387	the enantiomer separation	363:387	the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	363:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	2	46	theme	analytes	848:855	arg1	enantioselectivity					812:829	enantioselectivity	812:829	enantioselectivity	812:829	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	46	theme	analytes	848:855	arg1	characteristics					792:806	retention characteristics	782:806	retention characteristics	782:806	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	0	47	from	analogs	155:161	arg1	mode					210:213	liquid and subcritical fluid chromatography mode	166:213	liquid and subcritical fluid chromatography mode	166:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	7	48	theme	phases	1766:1771	arg1	selectors					1696:1704	selectors	1696:1704	selectors	1696:1704	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	7	48	theme	phases	1766:1771	arg1	properties					1741:1750	the properties	1737:1750	the properties of the mobile phases	1737:1771	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	7	48	theme	phases	1766:1771	arg1	structure					1711:1719	the structure	1707:1719	the structure of analytes	1707:1731	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	4	49	theme	bulk	1103:1106	arg1	solvent					1108:1114	bulk solvent	1103:1114	bulk solvent	1103:1114	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	8	50	theme	NP-LC	1827:1831	arg1	mode					1833:1836	NP-LC mode enthalpically-	1827:1851	NP-LC mode enthalpically-	1827:1851	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	1	51	theme	alkaloid-	587:595	arg1	phases					639:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases	531:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	531:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	1	51	theme	alkaloid-	587:595	arg1	CSPs					647:650	CSPs	647:650	CSPs	647:650	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	4	52	theme	chiral	1220:1225	arg1	sites					1236:1240	the chiral selector sites	1216:1240	the chiral selector sites which support the retention of ampholytes	1216:1282	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	7	53	theme	selectors	1696:1704	arg1	nature					1686:1691	the nature	1682:1691	the nature of selectors, the structure of analytes and the properties of the mobile phases	1682:1771	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	6	54	theme	entropy	1561:1567	arg1	ΔS°					1571:1573	ΔS°	1571:1573	ΔS°	1571:1573	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	54	theme	entropy	1561:1567	arg1	Δ					1569:1569	entropy Δ	1561:1569	entropy Δ(ΔS°)	1561:1574	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	8	55	theme	SFC	1865:1867	arg1	mode					1869:1872	SFC mode	1865:1872	SFC mode	1865:1872	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	2	56	theme	systematic	659:668	arg1	fashion					670:676	a systematic fashion	657:676	a systematic fashion	657:676	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	0	57	theme	indole	148:153	arg1	analogs					155:161	basic and ampholytic indole analogs	127:161	basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode	127:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	6	58	theme	enthalpy	1544:1551	arg1	ΔH°					1555:1557	ΔH°	1555:1557	ΔH°	1555:1557	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	58	theme	enthalpy	1544:1551	arg1	Δ					1553:1553	standard enthalpy Δ	1535:1553	standard enthalpy Δ(ΔH°)	1535:1558	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	59	used	used	1502:1505	arg2	Results					1442:1448	Results	1442:1448	Results acquired by studying the effect of temperature	1442:1495	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	8	60	theme	enantiomer	1918:1927	arg1	separations					1929:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	both enthalpically- and entropically-driven enantiomer separations	1874:1939	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	1	61	theme	ampholytic	414:423	arg1	analogs					469:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	6	62	theme	energy	1586:1591	arg1	ΔG°					1595:1597	ΔG°	1595:1597	ΔG°	1595:1597	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	62	theme	energy	1586:1591	arg1	Δ					1593:1593	free energy Δ	1581:1593	free energy Δ(ΔG°)	1581:1598	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	1	63	from	phases	639:644	arg1	separation					378:387	the enantiomer separation	363:387	the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	363:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	2	64	theme	nature	733:738	arg1	effect					682:687	the effect	678:687	the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes	678:855	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	0	65	theme	fluid	189:193	arg1	chromatography					195:208	liquid and subcritical fluid chromatography	166:208	chromatography	195:208	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	66	theme	related	438:444	arg1	analogs					469:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	2	67	theme	additives	765:773	arg1	effect					682:687	the effect	678:687	the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes	678:855	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	0	68	theme	chiral	90:95	arg1	phases					108:113	chiral stationary phases	90:113	chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode	90:213	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	1	69	theme	indole	462:467	arg1	analogs					469:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	three ampholytic structurally related C-3-substituted indole analogs	408:475	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	4	70	theme	selector	1227:1234	arg1	sites					1236:1240	the chiral selector sites	1216:1240	the chiral selector sites which support the retention of ampholytes	1216:1282	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	1	71	dep	polysaccharide-based	506:525	arg1	neutral					497:503	neutral	497:503	neutral	497:503	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	3	72	theme	studied	883:889	arg1	CSPs					912:915	all studied polysaccharide-based CSPs	879:915	all studied polysaccharide-based CSPs	879:915	On all studied polysaccharide-based CSPs the three ampholytes remained unretained in NP-LC mode, while they were nicely retained and resolved in SFC mode.
29895407	6	73	theme	Hoff	1615:1618	arg1	plots					1620:1624	van't Hoff plots	1609:1624	van't Hoff plots	1609:1624	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	74	theme	va	1609:1610	arg1	plots					1620:1624	van't Hoff plots	1609:1624	van't Hoff plots	1609:1624	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	5	75	theme	ionic	1397:1401	arg1	phase					1410:1414	a polar ionic mobile phase	1389:1414	a polar ionic mobile phase	1389:1414	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	6	76	theme	temperature	1485:1495	arg1	effect					1475:1480	the effect	1471:1480	the effect of temperature	1471:1495	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	7	77	theme	thermodynamic	1645:1657	arg1	parameters					1659:1668	the thermodynamic parameters	1641:1668	the thermodynamic parameters	1641:1668	The values of the thermodynamic parameters depended on the nature of selectors, the structure of analytes and the properties of the mobile phases.
29895407	2	78	theme	mobile	715:720	arg1	phase					722:726	the mobile phase	711:726	the mobile phase	711:726	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	1	79	theme	stationary	628:637	arg1	phases					639:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases	531:644	two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs)	531:651	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	1	79	theme	stationary	628:637	arg1	CSPs					647:650	CSPs	647:650	CSPs	647:650	Normal phase (NP) high-performance liquid and sub- and supercritical fluid chromatographic (both acronymed as SFC) methods have been developed for the enantiomer separation of three basic and three ampholytic structurally related C-3-substituted indole analogs on seven non-ionic (neutral) polysaccharide-based and two chemically entirely different zwitterionic Cinchona alkaloid- and sulfonic acid-based chiral stationary phases (CSPs).
29895407	0	80	theme	diverse	25:31	arg1	polysaccharide-					33:47	diverse polysaccharide-	25:47	diverse polysaccharide-	25:47	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	4	81	dep	combination	1119:1129	arg1	creating					1185:1192	creating	1185:1192	creating an environment around the chiral selector sites which support the retention of ampholytes	1185:1282	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	4	82	theme	liquid	1089:1094	arg1	CO2					1096:1098	liquid CO2	1089:1098	liquid CO2	1089:1098	These unexpected results underline a specific property of liquid CO2 as bulk solvent in combination with alcohols as co-solvents and amine additives thus creating an environment around the chiral selector sites which support the retention of ampholytes.
29895407	5	83	theme	analytes	1374:1381	arg1	resolution					1335:1344	the resolution	1331:1344	the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes	1331:1439	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	6	84	from	changes	1524:1530	arg1	Δ					1569:1569	entropy Δ	1561:1569	entropy Δ(ΔS°)	1561:1574	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	84	from	changes	1524:1530	arg1	Δ					1553:1553	standard enthalpy Δ	1535:1553	standard enthalpy Δ(ΔH°)	1535:1558	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	84	from	changes	1524:1530	arg1	Δ					1593:1593	free energy Δ	1581:1593	free energy Δ(ΔG°)	1581:1598	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	84	from	changes	1524:1530	arg1	ΔH°					1555:1557	ΔH°	1555:1557	ΔH°	1555:1557	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	84	from	changes	1524:1530	arg1	ΔS°					1571:1573	ΔS°	1571:1573	ΔS°	1571:1573	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	84	from	changes	1524:1530	arg1	ΔG°					1595:1597	ΔG°	1595:1597	ΔG°	1595:1597	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	0	85	theme	Cinchona	66:73	arg1	comparisons					10:20	Dedicated comparisons	0:20	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona	0:73	Dedicated comparisons of diverse polysaccharide- and zwitterionic Cinchona alkaloid-based chiral stationary phases probed with basic and ampholytic indole analogs in liquid and subcritical fluid chromatography mode.
29895407	3	86	theme	SFC	1021:1023	arg1	mode					1025:1028	SFC mode	1021:1028	SFC mode	1021:1028	On all studied polysaccharide-based CSPs the three ampholytes remained unretained in NP-LC mode, while they were nicely retained and resolved in SFC mode.
29895407	2	87	from	effect	682:687	arg1	enantioselectivity					812:829	enantioselectivity	812:829	enantioselectivity	812:829	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	2	87	from	effect	682:687	arg1	characteristics					792:806	retention characteristics	782:806	retention characteristics	782:806	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	6	88	theme	standard	1535:1542	arg1	ΔH°					1555:1557	ΔH°	1555:1557	ΔH°	1555:1557	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	6	88	theme	standard	1535:1542	arg1	Δ					1553:1553	standard enthalpy Δ	1535:1553	standard enthalpy Δ(ΔH°)	1535:1558	Results acquired by studying the effect of temperature were used to calculate the changes in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) applying van't Hoff plots.
29895407	8	89	dep	observed	1946:1953	arg1	whereas					1854:1860	whereas	1854:1860	whereas	1854:1860	On polysaccharide-based CSPs and columns operated in NP-LC mode enthalpically-, whereas in SFC mode both enthalpically- and entropically-driven enantiomer separations were observed.
29895407	2	90	dep	characteristics	792:806	arg1	the					778:780	the	778:780	the	778:780	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
29895407	5	91	theme	zwitterionic	1289:1300	arg1	CSPs					1302:1305	The zwitterionic CSPs	1285:1305	The zwitterionic CSPs	1285:1305	The zwitterionic CSPs worked equally well for the resolution of the basic and ampholytic analytes using a polar ionic mobile phase in both LC and SFC modes.
29895407	2	92	theme	ionizable	838:846	arg1	analytes					848:855	the ionizable analytes	834:855	the ionizable analytes	834:855	In a systematic fashion the effect of the composition of the mobile phase, the nature of the alcohol and amine additives on the retention characteristics and enantioselectivity of the ionizable analytes were investigated.
31151505	3	0	from	dose	674:677	arg1	composition					691:701	hydrogel composition	682:701	hydrogel composition	682:701	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	4	1	theme	Adsorption	704:713	arg1	data					715:718	Adsorption data	704:718	Adsorption data	704:718	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	6	2	from	water	1125:1129	arg1	removal					1099:1105	dye removal	1095:1105	dye removal from dyeing waste water	1095:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	2	3	theme	methyl	382:387	arg1	MV					397:398	MV	397:398	MV	397:398	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	2	3	theme	methyl	382:387	arg1	violet					389:394	methyl violet	382:394	methyl violet (MV)	382:399	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	5	4	theme	good	956:959	arg1	reversibility					961:973	a good reversibility	954:973	a good reversibility	954:973	Moreover, salecan-based hydrogels showed a good reversibility in adsorption-desorption cycles.
31151505	3	5	theme	hydrogel	682:689	arg1	composition					691:701	hydrogel composition	682:701	hydrogel composition	682:701	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	1	6	theme	economic	180:187	arg1	production					189:198	economic production	180:198	economic production	180:198	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	6	7	theme	waste	1119:1123	arg1	water					1125:1129	dyeing waste water	1112:1129	dyeing waste water	1112:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	4	8	theme	maximum	870:876	arg1	capacity					889:896	a maximum adsorption capacity	868:896	a maximum adsorption capacity of 178.9 mg/g	868:910	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	9	theme	Freundlich	822:831	arg1	R2 = 0.99221					849:860	R2 = 0.99221	849:860	R2 = 0.99221	849:860	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	9	theme	Freundlich	822:831	arg1	model					842:846	Freundlich isotherm model	822:846	Freundlich isotherm model (R2 = 0.99221)	822:861	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	10	theme	pseudo	769:774	arg1	model					797:801	the pseudo second-order kinetic model	765:801	the pseudo second-order kinetic model (R2 = 0.99015)	765:816	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	10	theme	pseudo	769:774	arg1	0.99015					809:815	0.99015	809:815	0.99015	809:815	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	2	11	theme	microbial	322:330	arg1	polysaccharide					332:345	a microbial polysaccharide	320:345	a microbial polysaccharide (salecan)	320:355	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	2	11	theme	microbial	322:330	arg1	salecan					348:354	salecan	348:354	salecan	348:354	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	3	12	theme	adsorbent	553:561	arg1	properties					450:459	the properties	446:459	the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake)	446:544	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	3	12	theme	adsorbent	553:561	arg1	fabrication					430:440	The success fabrication	418:440	The success fabrication	418:440	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	5	13	theme	salecan-based	923:935	arg1	hydrogels					937:945	salecan-based hydrogels	923:945	salecan-based hydrogels	923:945	Moreover, salecan-based hydrogels showed a good reversibility in adsorption-desorption cycles.
31151505	6	14	theme	promising	1074:1082	arg1	device					1084:1089	a promising device	1072:1089	a promising device for dye removal from dyeing waste water	1072:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	6	14	theme	promising	1074:1082	arg1	bio-adsorbent					1051:1063	salecan-based bio-adsorbent	1037:1063	salecan-based bio-adsorbent	1037:1063	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	1	15	theme	Wastewater	86:95	arg1	materials					107:115	Wastewater treatment materials	86:115	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production	86:198	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	3	16	theme	success	422:428	arg1	fabrication					430:440	The success fabrication	418:440	The success fabrication	418:440	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	1	17	theme	treatment	97:105	arg1	materials					107:115	Wastewater treatment materials	86:115	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production	86:198	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	6	18	theme	dyeing	1112:1117	arg1	water					1125:1129	dyeing waste water	1112:1129	dyeing waste water	1112:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	0	19	theme	efficient	7:15	arg1	decontamination					21:35	Highly efficient dye decontamination	0:35	Highly efficient dye decontamination	0:35	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	6	20	theme	dye	1095:1097	arg1	removal					1099:1105	dye removal	1095:1105	dye removal from dyeing waste water	1095:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	3	21	theme	thermal	470:476	arg1	stability					478:486	thermal stability	470:486	thermal stability	470:486	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	0	22	theme	dye	17:19	arg1	decontamination					21:35	Highly efficient dye decontamination	0:35	Highly efficient dye decontamination	0:35	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	4	23	theme	kinetic	789:795	arg1	model					797:801	the pseudo second-order kinetic model	765:801	the pseudo second-order kinetic model (R2 = 0.99015)	765:816	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	23	theme	kinetic	789:795	arg1	0.99015					809:815	0.99015	809:815	0.99015	809:815	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	1	24	theme	high	130:133	arg1	performance					151:161	high decontamination performance	130:161	high decontamination performance	130:161	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	1	25	theme	practical	225:233	arg1	applications					235:246	practical applications	225:246	practical applications	225:246	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	0	26	theme	microbial	41:49	arg1	gels					80:83	microbial salecan polysaccharide-based gels	41:83	microbial salecan polysaccharide-based gels	41:83	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	3	27	theme	mechanical	508:517	arg1	strength					519:526	mechanical strength	508:526	mechanical strength	508:526	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	0	28	theme	polysaccharide-based	59:78	arg1	gels					80:83	microbial salecan polysaccharide-based gels	41:83	microbial salecan polysaccharide-based gels	41:83	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	5	29	theme	adsorption-desorption	978:998	arg1	cycles					1000:1005	adsorption-desorption cycles	978:1005	adsorption-desorption cycles	978:1005	Moreover, salecan-based hydrogels showed a good reversibility in adsorption-desorption cycles.
31151505	3	30	dep	properties	450:459	arg1	such					462:465	such	462:465	such	462:465	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	1	31	theme	decontamination	135:149	arg1	performance					151:161	high decontamination performance	130:161	high decontamination performance	130:161	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	0	32	theme	salecan	51:57	arg1	gels					80:83	microbial salecan polysaccharide-based gels	41:83	microbial salecan polysaccharide-based gels	41:83	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	4	33	theme	adsorption	878:887	arg1	capacity					889:896	a maximum adsorption capacity	868:896	a maximum adsorption capacity of 178.9 mg/g	868:910	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	34	theme	isotherm	833:840	arg1	R2 = 0.99221					849:860	R2 = 0.99221	849:860	R2 = 0.99221	849:860	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	34	theme	isotherm	833:840	arg1	model					842:846	Freundlich isotherm model	822:846	Freundlich isotherm model (R2 = 0.99221)	822:861	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	2	35	from	wastewater	406:415	arg1	removal					371:377	efficient removal	361:377	efficient removal of methyl violet (MV) from wastewater	361:415	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	2	36	theme	efficient	361:369	arg1	removal					371:377	efficient removal	361:377	efficient removal of methyl violet (MV) from wastewater	361:415	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	4	37	theme	178.9 mg/g	901:910	arg1	capacity					889:896	a maximum adsorption capacity	868:896	a maximum adsorption capacity of 178.9 mg/g	868:910	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	0	38	dep	decontamination	21:35	arg1	gels					80:83	microbial salecan polysaccharide-based gels	41:83	microbial salecan polysaccharide-based gels	41:83	Highly efficient dye decontamination via microbial salecan polysaccharide-based gels.
31151505	2	39	theme	recyclable	286:295	arg1	bio-adsorbent					297:309	a low-cost and recyclable bio-adsorbent	271:309	a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan)	271:355	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	4	40	theme	second-order	776:787	arg1	model					797:801	the pseudo second-order kinetic model	765:801	the pseudo second-order kinetic model (R2 = 0.99015)	765:816	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	4	40	theme	second-order	776:787	arg1	0.99015					809:815	0.99015	809:815	0.99015	809:815	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	3	41	theme	tunable	623:629	arg1	properties					631:640	tunable properties	623:640	tunable properties	623:640	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	4	42	theme	MV	753:754	arg1	adsorption					739:748	the adsorption	735:748	the adsorption of MV	735:754	Adsorption data displayed that the adsorption of MV followed the pseudo second-order kinetic model (R2 = 0.99015) and Freundlich isotherm model (R2 = 0.99221) with a maximum adsorption capacity of 178.9 mg/g.
31151505	1	43	theme	use	172:174	arg1	production					189:198	economic production	180:198	economic production	180:198	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	1	43	theme	use	172:174	arg1	ease					164:167	ease	164:167	ease of use	164:174	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	1	43	theme	use	172:174	arg1	performance					151:161	high decontamination performance	130:161	high decontamination performance	130:161	Wastewater treatment materials that combine high decontamination performance, ease of use and economic production are highly desirable for practical applications.
31151505	3	44	theme	salecan	666:672	arg1	dose					674:677	the salecan dose	662:677	the salecan dose in hydrogel composition	662:701	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	2	45	theme	low-cost	273:280	arg1	bio-adsorbent					297:309	a low-cost and recyclable bio-adsorbent	271:309	a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan)	271:355	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	3	46	theme	water	532:536	arg1	uptake					538:543	water uptake	532:543	water uptake	532:543	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	6	47	theme	salecan-based	1037:1049	arg1	device					1084:1089	a promising device	1072:1089	a promising device for dye removal from dyeing waste water	1072:1129	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	6	47	theme	salecan-based	1037:1049	arg1	bio-adsorbent					1051:1063	salecan-based bio-adsorbent	1037:1063	salecan-based bio-adsorbent	1037:1063	These features indicate that salecan-based bio-adsorbent may be a promising device for dye removal from dyeing waste water.
31151505	2	48	theme	violet	389:394	arg1	removal					371:377	efficient removal	361:377	efficient removal of methyl violet (MV) from wastewater	361:415	Herein, we fabricated a low-cost and recyclable bio-adsorbent based on a microbial polysaccharide (salecan) for efficient removal of methyl violet (MV) from wastewater.
31151505	3	49	contain	have	618:621	arg1	hydrogels					594:602	the hydrogels	590:602	the hydrogels	590:602	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
31151505	3	49	contain	have	618:621	arg2	properties					631:640	tunable properties	623:640	tunable properties	623:640	The success fabrication and the properties (such as thermal stability, microarchitecture, mechanical strength and water uptake) of the adsorbent had been investigated, and the hydrogels were found to have tunable properties by simple adjusting the salecan dose in hydrogel composition.
30031081	10	0	theme	mole	1181:1184	arg1	different					1246:1254	different	1246:1254	different	1246:1254	The mole ratio of the monosaccharide composition of four samples was different.
30031081	10	0	theme	mole	1181:1184	arg1	ratio					1186:1190	The mole ratio	1177:1190	The mole ratio of the monosaccharide composition of four samples	1177:1240	The mole ratio of the monosaccharide composition of four samples was different.
30031081	12	1	from	edodes	1491:1496	arg1	polysaccharides					1461:1475	polysaccharides	1461:1475	polysaccharides from Lentinus edodes	1461:1496	These results revealed that USWE was an effective technique for extracting and separating polysaccharides from Lentinus edodes, which may be potentially applied in food, cosmetics, and medicines.
30031081	6	2	theme	ultrasonic	821:830	arg1	extraction					832:841	ultrasonic extraction	821:841	ultrasonic extraction (UE)	821:846	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	2	theme	ultrasonic	821:830	arg1	UE					844:845	UE	844:845	UE	844:845	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	8	3	theme	DPPH	1010:1013	arg1	rate					1026:1029	DPPH scavenging rate	1010:1029	DPPH scavenging rate of HWE, SWE, UE, and USWE sample	1010:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	9	4	contain	had	1136:1138	arg1	sample					1129:1134	The USWE sample	1120:1134	The USWE sample	1120:1134	The USWE sample had the highest reducing power activity.
30031081	9	4	contain	had	1136:1138	arg2	activity					1167:1174	the highest reducing power activity	1140:1174	the highest reducing power activity	1140:1174	The USWE sample had the highest reducing power activity.
30031081	0	5	theme	Lentinus	111:118	arg1	edodes					120:125	Lentinus edodes	111:125	Lentinus edodes	111:125	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	5	6	theme	maximum	651:657	arg1	%					703:703	17.34%	698:703	17.34%	698:703	The maximum extraction rate of polysaccharides was 17.34%.
30031081	5	6	theme	maximum	651:657	arg1	rate					670:673	The maximum extraction rate	647:673	The maximum extraction rate of polysaccharides	647:692	The maximum extraction rate of polysaccharides was 17.34%.
30031081	2	7	theme	extraction	301:310	arg1	method					312:317	a novel extraction method	293:317	a novel extraction method	293:317	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	4	8	theme	USWE	587:590	arg1	condition					592:600	the USWE condition	583:600	the USWE condition based on the results of single-factor tests	583:644	A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests.
30031081	11	9	theme	polysaccharides	1323:1337	arg1	morphology					1300:1309	the morphology	1296:1309	the morphology of the four polysaccharides	1296:1337	The results of AFM and SEM showed that the morphology of the four polysaccharides showed significant differences.
30031081	5	10	theme	extraction	659:668	arg1	%					703:703	17.34%	698:703	17.34%	698:703	The maximum extraction rate of polysaccharides was 17.34%.
30031081	5	10	theme	extraction	659:668	arg1	rate					670:673	The maximum extraction rate	647:673	The maximum extraction rate of polysaccharides	647:692	The maximum extraction rate of polysaccharides was 17.34%.
30031081	2	11	theme	novel	295:299	arg1	method					312:317	a novel extraction method	293:317	a novel extraction method	293:317	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	10	12	theme	composition	1214:1224	arg1	different					1246:1254	different	1246:1254	different	1246:1254	The mole ratio of the monosaccharide composition of four samples was different.
30031081	10	12	theme	composition	1214:1224	arg1	ratio					1186:1190	The mole ratio	1177:1190	The mole ratio of the monosaccharide composition of four samples	1177:1240	The mole ratio of the monosaccharide composition of four samples was different.
30031081	11	13	theme	significant	1346:1356	arg1	differences					1358:1368	significant differences	1346:1368	significant differences	1346:1368	The results of AFM and SEM showed that the morphology of the four polysaccharides showed significant differences.
30031081	8	14	theme	scavenging	1015:1024	arg1	rate					1026:1029	DPPH scavenging rate	1010:1029	DPPH scavenging rate of HWE, SWE, UE, and USWE sample	1010:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	3	15	theme	ultrasonic	500:509	arg1	power					511:515	ultrasonic power	500:515	ultrasonic power	500:515	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	15	theme	ultrasonic	500:509	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	6	16	theme	extraction	803:812	arg1	samples					880:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	10	17	theme	monosaccharide	1199:1212	arg1	composition					1214:1224	the monosaccharide composition	1195:1224	the monosaccharide composition of four samples	1195:1240	The mole ratio of the monosaccharide composition of four samples was different.
30031081	1	18	theme	chief	188:192	arg1	polysaccharides					164:178	polysaccharides	164:178	polysaccharides	164:178	Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes.
30031081	1	18	theme	chief	188:192	arg1	ingredient					194:203	the chief ingredient	184:203	the chief ingredient of Lentinus edodes	184:222	Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes.
30031081	11	19	theme	AFM	1272:1274	arg1	results					1261:1267	The results	1257:1267	The results of AFM and SEM	1257:1282	The results of AFM and SEM showed that the morphology of the four polysaccharides showed significant differences.
30031081	6	20	theme	water	857:861	arg1	extraction					863:872	hot water extraction	853:872	hot water extraction (HWE)	853:878	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	20	theme	water	857:861	arg1	HWE					875:877	HWE	875:877	HWE	875:877	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	21	theme	water	797:801	arg1	SWE					815:817	SWE	815:817	SWE	815:817	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	21	theme	water	797:801	arg1	extraction					803:812	subcritical water extraction	785:812	subcritical water extraction (SWE)	785:818	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	3	22	theme	extraction	456:465	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	22	theme	extraction	456:465	arg1	time					467:470	extraction time	456:470	extraction time	456:470	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	0	23	theme	subcritical	20:30	arg1	extraction					38:47	Ultrasonic-enhanced subcritical water extraction	0:47	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps	0:79	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	6	24	theme	hot	853:855	arg1	extraction					863:872	hot water extraction	853:872	hot water extraction (HWE)	853:878	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	24	theme	hot	853:855	arg1	HWE					875:877	HWE	875:877	HWE	875:877	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	3	25	dep	factors	423:429	arg1	power					511:515	ultrasonic power	500:515	ultrasonic power	500:515	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	25	dep	factors	423:429	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	25	dep	factors	423:429	arg1	temperature					443:453	extraction temperature	432:453	extraction temperature	432:453	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	25	dep	factors	423:429	arg1	time					467:470	extraction time	456:470	extraction time	456:470	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	25	dep	factors	423:429	arg1	ratio					489:493	liquid-to-solid ratio	473:493	liquid-to-solid ratio	473:493	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	6	26	theme	subcritical	785:795	arg1	SWE					815:817	SWE	815:817	SWE	815:817	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	26	theme	subcritical	785:795	arg1	extraction					803:812	subcritical water extraction	785:812	subcritical water extraction (SWE)	785:818	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	27	theme	extraction	863:872	arg1	samples					880:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	3	28	theme	extraction	432:441	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	28	theme	extraction	432:441	arg1	temperature					443:453	extraction temperature	432:453	extraction temperature	432:453	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	0	29	theme	Ultrasonic-enhanced	0:18	arg1	extraction					38:47	Ultrasonic-enhanced subcritical water extraction	0:47	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps	0:79	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	8	30	theme	IC50	995:998	arg1	value					1000:1004	The IC50 value	991:1004	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample	991:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	8	30	theme	IC50	995:998	arg1	0.341					1068:1072	0.341	1068:1072	0.341	1068:1072	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	2	31	from	edodes	277:282	arg1	polysaccharides					247:261	the useful polysaccharides	236:261	the useful polysaccharides from Lentinus edodes	236:282	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	1	32	theme	Lentinus	208:215	arg1	edodes					217:222	Lentinus edodes	208:222	Lentinus edodes	208:222	Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes.
30031081	10	33	theme	samples	1234:1240	arg1	composition					1214:1224	the monosaccharide composition	1195:1224	the monosaccharide composition of four samples	1195:1240	The mole ratio of the monosaccharide composition of four samples was different.
30031081	3	34	theme	liquid-to-solid	473:487	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	34	theme	liquid-to-solid	473:487	arg1	ratio					489:493	liquid-to-solid ratio	473:493	liquid-to-solid ratio	473:493	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	0	35	theme	water	32:36	arg1	extraction					38:47	Ultrasonic-enhanced subcritical water extraction	0:47	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps	0:79	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	8	36	theme	SWE	1039:1041	arg1	sample					1057:1062	HWE, SWE, UE, and USWE sample	1034:1062	HWE, SWE, UE, and USWE sample	1034:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	9	37	theme	highest	1144:1150	arg1	activity					1167:1174	the highest reducing power activity	1140:1174	the highest reducing power activity	1140:1174	The USWE sample had the highest reducing power activity.
30031081	0	38	theme	polysaccharides	52:66	arg1	extraction					38:47	Ultrasonic-enhanced subcritical water extraction	0:47	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps	0:79	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	0	38	theme	polysaccharides	52:66	arg1	characterization					89:104	its characterization	85:104	its characterization from Lentinus edodes	85:125	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	12	39	theme	effective	1411:1419	arg1	USWE					1399:1402	USWE	1399:1402	USWE	1399:1402	These results revealed that USWE was an effective technique for extracting and separating polysaccharides from Lentinus edodes, which may be potentially applied in food, cosmetics, and medicines.
30031081	12	39	theme	effective	1411:1419	arg1	technique					1421:1429	an effective technique	1408:1429	an effective technique	1408:1429	These results revealed that USWE was an effective technique for extracting and separating polysaccharides from Lentinus edodes, which may be potentially applied in food, cosmetics, and medicines.
30031081	9	40	theme	reducing	1152:1159	arg1	activity					1167:1174	the highest reducing power activity	1140:1174	the highest reducing power activity	1140:1174	The USWE sample had the highest reducing power activity.
30031081	4	41	theme	tests	640:644	arg1	results					615:621	the results	611:621	the results of single-factor tests	611:644	A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests.
30031081	11	42	theme	SEM	1280:1282	arg1	results					1261:1267	The results	1257:1267	The results of AFM and SEM	1257:1282	The results of AFM and SEM showed that the morphology of the four polysaccharides showed significant differences.
30031081	4	43	theme	Box-Behnken	534:544	arg1	design					546:551	A Box-Behnken design	532:551	A Box-Behnken design (BBD)	532:557	A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests.
30031081	4	43	theme	Box-Behnken	534:544	arg1	BBD					554:556	BBD	554:556	BBD	554:556	A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests.
30031081	8	44	theme	UE	1044:1045	arg1	sample					1057:1062	HWE, SWE, UE, and USWE sample	1034:1062	HWE, SWE, UE, and USWE sample	1034:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	9	45	theme	power	1161:1165	arg1	activity					1167:1174	the highest reducing power activity	1140:1174	the highest reducing power activity	1140:1174	The USWE sample had the highest reducing power activity.
30031081	4	46	theme	single-factor	626:638	arg1	tests					640:644	single-factor tests	626:644	single-factor tests	626:644	A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests.
30031081	8	47	theme	USWE	1052:1055	arg1	sample					1057:1062	HWE, SWE, UE, and USWE sample	1034:1062	HWE, SWE, UE, and USWE sample	1034:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	2	48	theme	useful	240:245	arg1	polysaccharides					247:261	the useful polysaccharides	236:261	the useful polysaccharides from Lentinus edodes	236:282	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	2	49	theme	water	368:372	arg1	extraction					374:383	ultrasonic-enhanced subcritical water extraction	336:383	ultrasonic-enhanced subcritical water extraction by two steps (USWE)	336:403	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	3	50	theme	independent	411:421	arg1	power					511:515	ultrasonic power	500:515	ultrasonic power	500:515	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	50	theme	independent	411:421	arg1	factors					423:429	Four independent factors	406:429	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power)	406:516	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	50	theme	independent	411:421	arg1	temperature					443:453	extraction temperature	432:453	extraction temperature	432:453	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	50	theme	independent	411:421	arg1	time					467:470	extraction time	456:470	extraction time	456:470	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	3	50	theme	independent	411:421	arg1	ratio					489:493	liquid-to-solid ratio	473:493	liquid-to-solid ratio	473:493	Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied.
30031081	9	51	theme	USWE	1124:1127	arg1	sample					1129:1134	The USWE sample	1120:1134	The USWE sample	1120:1134	The USWE sample had the highest reducing power activity.
30031081	6	52	theme	extraction	832:841	arg1	samples					880:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples	785:886	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	53	theme	optimal	748:754	arg1	condition					756:764	optimal condition	748:764	optimal condition	748:764	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	2	54	theme	subcritical	356:366	arg1	extraction					374:383	ultrasonic-enhanced subcritical water extraction	336:383	ultrasonic-enhanced subcritical water extraction by two steps (USWE)	336:403	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	5	55	theme	polysaccharides	678:692	arg1	%					703:703	17.34%	698:703	17.34%	698:703	The maximum extraction rate of polysaccharides was 17.34%.
30031081	5	55	theme	polysaccharides	678:692	arg1	rate					670:673	The maximum extraction rate	647:673	The maximum extraction rate of polysaccharides	647:692	The maximum extraction rate of polysaccharides was 17.34%.
30031081	0	56	from	edodes	120:125	arg1	extraction					38:47	Ultrasonic-enhanced subcritical water extraction	0:47	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps	0:79	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	0	56	from	edodes	120:125	arg1	characterization					89:104	its characterization	85:104	its characterization from Lentinus edodes	85:125	Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes.
30031081	2	57	theme	ultrasonic-enhanced	336:354	arg1	extraction					374:383	ultrasonic-enhanced subcritical water extraction	336:383	ultrasonic-enhanced subcritical water extraction by two steps (USWE)	336:403	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	8	58	theme	sample	1057:1062	arg1	rate					1026:1029	DPPH scavenging rate	1010:1029	DPPH scavenging rate of HWE, SWE, UE, and USWE sample	1010:1062	The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively.
30031081	6	59	theme	extracted	719:727	arg1	polysaccharides					729:743	extracted polysaccharides	719:743	extracted polysaccharides at optimal condition	719:764	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	6	60	from	condition	756:764	arg1	polysaccharides					729:743	extracted polysaccharides	719:743	extracted polysaccharides at optimal condition	719:764	In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples.
30031081	1	61	theme	edodes	217:222	arg1	polysaccharides					164:178	polysaccharides	164:178	polysaccharides	164:178	Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes.
30031081	1	61	theme	edodes	217:222	arg1	ingredient					194:203	the chief ingredient	184:203	the chief ingredient of Lentinus edodes	184:222	Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes.
30031081	12	62	theme	Lentinus	1482:1489	arg1	edodes					1491:1496	Lentinus edodes	1482:1496	Lentinus edodes	1482:1496	These results revealed that USWE was an effective technique for extracting and separating polysaccharides from Lentinus edodes, which may be potentially applied in food, cosmetics, and medicines.
30031081	2	63	theme	Lentinus	268:275	arg1	edodes					277:282	Lentinus edodes	268:282	Lentinus edodes	268:282	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
30031081	7	64	theme	reducing	955:962	arg1	power					964:968	reducing power	955:968	reducing power	955:968	The antioxidant against 2,2‑diphenyl‑1‑picryl‑hydrazyl (DPPH) and reducing power were also evaluated.
30031081	2	65	used	used	288:291	arg2	we					285:286	we	285:286	we	285:286	To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE).
29105226	3	0	theme	N-glycan	714:721	arg1	identification					723:736	automated N-glycan identification	704:736	automated N-glycan identification	704:736	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	8	1	theme	comprehensive	1794:1806	arg1	interpretation					1808:1821	comprehensive interpretation	1794:1821	comprehensive interpretation of tandem mass spectra and FDR control	1794:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	7	2	theme	large-scale	1525:1535	arg1	identification					1537:1550	large-scale identification	1525:1550	large-scale identification	1525:1550	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	6	3	theme	best	1253:1256	arg1	NoBHs					1264:1268	NoBHs	1264:1268	NoBHs	1264:1268	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	3	theme	best	1253:1256	arg1	hits					1258:1261	best hits	1253:1261	best hits (NoBHs)	1253:1269	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	1	4	theme	current	121:127	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	5	contain	have	163:166	arg2	capability					172:181	the capability	168:181	the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans	168:281	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	5	contain	have	163:166	arg1	Most					109:112	Most	109:112	Most	109:112	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	5	contain	have	163:166	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	6	6	theme	primary	1313:1319	arg1	information					1332:1342	comprehensive primary structural information	1299:1342	comprehensive primary structural information (composition, sequence and linkage)	1299:1378	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	7	theme	mass	1833:1836	arg1	spectra					1838:1844	tandem mass spectra	1826:1844	tandem mass spectra	1826:1844	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	6	8	dep	%	1237:1237	arg1	1-30					1273:1276	= 1-30	1271:1276	= 1-30	1271:1276	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	1	9	theme	primary	200:206	arg1	linkage					261:267	linkage	261:267	linkage	261:267	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	9	theme	primary	200:206	arg1	sequence					248:255	sequence	248:255	sequence	248:255	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	9	theme	primary	200:206	arg1	structures					208:217	the primary structures	196:217	the primary structures (monosaccharide composition, sequence and linkage) of N-glycans	196:281	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	9	theme	primary	200:206	arg1	composition					235:245	monosaccharide composition	220:245	monosaccharide composition	220:245	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	6	10	theme	FDR	1231:1233	arg1	NoBHs					1264:1268	NoBHs	1264:1268	NoBHs	1264:1268	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	10	theme	FDR	1231:1233	arg1	hits					1258:1261	best hits	1253:1261	best hits (NoBHs)	1253:1269	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	10	theme	FDR	1231:1233	arg1	%					1237:1237	FDR ≤1%	1231:1237	FDR ≤1%	1231:1237	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	11	theme	control	1854:1860	arg1	interpretation					1808:1821	comprehensive interpretation	1794:1821	comprehensive interpretation of tandem mass spectra and FDR control	1794:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	4	12	dep	Both	800:803	arg1	graph					815:819	pseudo 2D graph	805:819	pseudo 2D graph	805:819	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	8	13	dep	CONCLUSIONS	1633:1643	arg1	developed					1717:1725	developed	1717:1725	has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control	1708:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	3	14	theme	database	621:628	arg1	search					630:635	N-glycan database search	612:635	N-glycan database search	612:635	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	5	15	theme	ions	1004:1007	arg1	interpretation					978:991	comprehensive interpretation	964:991	comprehensive interpretation of product ions	964:1007	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	5	15	theme	ions	1004:1007	arg1	control					1040:1046	false discovery rate (FDR) control	1013:1046	false discovery rate (FDR) control	1013:1046	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	3	16	theme	N-glycan	656:663	arg1	engine					681:686	a stand-alone N-glycan database search engine	642:686	a stand-alone N-glycan database search engine	642:686	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	16	theme	N-glycan	656:663	arg1	GlySeeker					689:697	GlySeeker	689:697	GlySeeker	689:697	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	5	17	theme	false	1013:1017	arg1	FDR					1035:1037	FDR	1035:1037	FDR	1035:1037	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	5	17	theme	false	1013:1017	arg1	rate					1029:1032	false discovery rate	1013:1032	false discovery rate (FDR) control	1013:1046	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	6	18	theme	liquid	1096:1101	arg1	RPLC/MS/MS					1144:1153	RPLC/MS/MS	1144:1153	RPLC/MS/MS	1144:1153	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	18	theme	liquid	1096:1101	arg1	spectrometry					1130:1141	reversed-phase liquid chromatography/tandem mass spectrometry	1081:1141	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	5	19	theme	comprehensive	964:976	arg1	interpretation					978:991	comprehensive interpretation	964:991	comprehensive interpretation of product ions	964:1007	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	6	20	dep	=	1454:1454	arg1	1					1456:1456	1	1456:1456	1	1456:1456	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	21	theme	database	1062:1069	arg1	search					1071:1076	a database search	1060:1076	a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1060:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	4	22	with	formats	839:845	arg1	symbols					863:869	one-letter symbols	852:869	one-letter symbols for monosaccharides	852:889	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	1	23	theme	mass	144:147	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	3	24	dep	METHODS	427:433	arg1	ratio					463:467	The isotopic mass-to-charge ratio	435:467	The isotopic mass-to-charge ratio	435:467	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	24	dep	METHODS	427:433	arg1	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	24	dep	METHODS	427:433	arg1	algorithm					497:505	envelope fingerprinting algorithm	473:505	envelope fingerprinting algorithm	473:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	25	theme	isotopic	439:446	arg1	ratio					463:467	The isotopic mass-to-charge ratio	435:467	The isotopic mass-to-charge ratio	435:467	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	25	theme	isotopic	439:446	arg1	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	0	26	theme	Large-scale	0:10	arg1	identification					12:25	identification	12:25	identification	12:25	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	5	27	theme	rate	1029:1032	arg1	control					1040:1046	false discovery rate (FDR) control	1013:1046	false discovery rate (FDR) control	1013:1046	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	8	28	with	visualization	1762:1774	arg1	interpretation					1808:1821	comprehensive interpretation	1794:1821	comprehensive interpretation of tandem mass spectra and FDR control	1794:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	4	29	theme	pseudo	805:810	arg1	graph					815:819	pseudo 2D graph	805:819	pseudo 2D graph	805:819	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	7	30	dep	identification	1537:1550	arg1	a					1523:1523	a	1523:1523	a	1523:1523	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	7	31	from	spectra	1624:1630	arg1	structures					1596:1605	primary structures	1588:1605	primary structures from tandem mass spectra	1588:1630	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	6	32	from	RESULTS	1049:1055	arg1	search					1071:1076	a database search	1060:1076	a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1060:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	33	theme	envelope	473:480	arg1	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	33	theme	envelope	473:480	arg1	algorithm					497:505	envelope fingerprinting algorithm	473:505	envelope fingerprinting algorithm	473:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	1	34	theme	putative	309:316	arg1	structures					318:327	putative structures	309:327	putative structures	309:327	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	7	35	theme	primary	1588:1594	arg1	structures					1596:1605	primary structures	1588:1605	primary structures from tandem mass spectra	1588:1630	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	6	36	theme	reversed-phase	1081:1094	arg1	RPLC/MS/MS					1144:1153	RPLC/MS/MS	1144:1153	RPLC/MS/MS	1144:1153	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	36	theme	reversed-phase	1081:1094	arg1	spectrometry					1130:1141	reversed-phase liquid chromatography/tandem mass spectrometry	1081:1141	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	37	theme	mass	1125:1128	arg1	RPLC/MS/MS					1144:1153	RPLC/MS/MS	1144:1153	RPLC/MS/MS	1144:1153	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	37	theme	mass	1125:1128	arg1	spectrometry					1130:1141	reversed-phase liquid chromatography/tandem mass spectrometry	1081:1141	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	38	theme	search	674:679	arg1	engine					681:686	a stand-alone N-glycan database search engine	642:686	a stand-alone N-glycan database search engine	642:686	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	38	theme	search	674:679	arg1	GlySeeker					689:697	GlySeeker	689:697	GlySeeker	689:697	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	6	39	theme	=	1454:1454	arg1	NoBHs					1448:1452	NoBHs	1448:1452	NoBHs = 1	1448:1456	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	7	40	theme	N-glycans	1573:1581	arg1	visualization					1556:1568	visualization	1556:1568	visualization	1556:1568	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	7	40	theme	N-glycans	1573:1581	arg1	identification					1537:1550	large-scale identification	1525:1550	large-scale identification	1525:1550	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	8	41	theme	N-glycan	1659:1666	arg1	engine					1684:1689	A stand-alone N-glycan database search engine	1645:1689	A stand-alone N-glycan database search engine called GlySeeker	1645:1706	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	3	42	theme	automated	704:712	arg1	identification					723:736	automated N-glycan identification	704:736	automated N-glycan identification	704:736	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	8	43	theme	large-scale	1731:1741	arg1	identification					1743:1756	large-scale identification	1731:1756	large-scale identification	1731:1756	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	6	44	theme	ovarian	1204:1210	arg1	cells					1219:1223	OVCAR-3 ovarian cancer cells	1196:1223	OVCAR-3 ovarian cancer cells	1196:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	45	theme	search	1677:1682	arg1	engine					1684:1689	A stand-alone N-glycan database search engine	1645:1689	A stand-alone N-glycan database search engine called GlySeeker	1645:1706	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	4	46	theme	one-letter	852:861	arg1	symbols					863:869	one-letter symbols	852:869	one-letter symbols for monosaccharides	852:889	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	3	47	theme	intact	545:550	arg1	search					569:574	intact protein database search	545:574	intact protein database search	545:574	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	6	48	theme	N-glycome	1172:1180	arg1	datasets					1156:1163	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets	1081:1163	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	1	49	theme	N-glycans	273:281	arg1	linkage					261:267	linkage	261:267	linkage	261:267	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	49	theme	N-glycans	273:281	arg1	sequence					248:255	sequence	248:255	sequence	248:255	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	49	theme	N-glycans	273:281	arg1	structures					208:217	the primary structures	196:217	the primary structures (monosaccharide composition, sequence and linkage) of N-glycans	196:281	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	49	theme	N-glycans	273:281	arg1	composition					235:245	monosaccharide composition	220:245	monosaccharide composition	220:245	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	6	50	dep	information	1332:1342	arg1	linkage					1371:1377	linkage	1371:1377	linkage	1371:1377	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	50	dep	information	1332:1342	arg1	sequence					1358:1365	sequence	1358:1365	sequence	1358:1365	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	50	dep	information	1332:1342	arg1	composition					1345:1355	composition	1345:1355	composition	1345:1355	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	51	theme	structural	1321:1330	arg1	information					1332:1342	comprehensive primary structural information	1299:1342	comprehensive primary structural information (composition, sequence and linkage)	1299:1378	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	52	theme	database	560:567	arg1	search					569:574	intact protein database search	545:574	intact protein database search	545:574	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	6	53	theme	comprehensive	1299:1311	arg1	information					1332:1342	comprehensive primary structural information	1299:1342	comprehensive primary structural information (composition, sequence and linkage)	1299:1378	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	54	theme	tandem	1826:1831	arg1	spectra					1838:1844	tandem mass spectra	1826:1844	tandem mass spectra	1826:1844	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	4	55	dep	formats	839:845	arg1	Both					800:803	Both	800:803	Both	800:803	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	4	55	dep	formats	839:845	arg1	one-line					825:832	one-line	825:832	one-line	825:832	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	6	56	theme	≤1	1235:1236	arg1	NoBHs					1264:1268	NoBHs	1264:1268	NoBHs	1264:1268	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	56	theme	≤1	1235:1236	arg1	hits					1258:1261	best hits	1253:1261	best hits (NoBHs)	1253:1269	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	56	theme	≤1	1235:1236	arg1	%					1237:1237	FDR ≤1%	1231:1237	FDR ≤1%	1231:1237	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	57	theme	spectra	1838:1844	arg1	interpretation					1808:1821	comprehensive interpretation	1794:1821	comprehensive interpretation of tandem mass spectra and FDR control	1794:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	8	58	theme	FDR	1850:1852	arg1	control					1854:1860	FDR control	1850:1860	FDR control	1850:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	1	59	theme	monosaccharide	220:233	arg1	structures					208:217	the primary structures	196:217	the primary structures (monosaccharide composition, sequence and linkage) of N-glycans	196:281	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	59	theme	monosaccharide	220:233	arg1	composition					235:245	monosaccharide composition	220:245	monosaccharide composition	220:245	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	3	60	theme	N-glycan	612:619	arg1	search					630:635	N-glycan database search	612:635	N-glycan database search	612:635	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	5	61	theme	product	996:1002	arg1	ions					1004:1007	product ions	996:1007	product ions	996:1007	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	6	62	theme	=	1271:1271	arg1	1-30					1273:1276	= 1-30	1271:1276	= 1-30	1271:1276	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	63	theme	stand-alone	644:654	arg1	engine					681:686	a stand-alone N-glycan database search engine	642:686	a stand-alone N-glycan database search engine	642:686	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	63	theme	stand-alone	644:654	arg1	GlySeeker					689:697	GlySeeker	689:697	GlySeeker	689:697	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	1	64	theme	tandem	137:142	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	6	65	theme	chromatography/tandem	1103:1123	arg1	RPLC/MS/MS					1144:1153	RPLC/MS/MS	1144:1153	RPLC/MS/MS	1144:1153	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	65	theme	chromatography/tandem	1103:1123	arg1	spectrometry					1130:1141	reversed-phase liquid chromatography/tandem mass spectrometry	1081:1141	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	1	66	theme	spectrometers	149:161	arg1	Most					109:112	Most	109:112	Most	109:112	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	66	theme	spectrometers	149:161	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	67	theme	popular	129:135	arg1	spectrometers					149:161	the current popular tandem mass spectrometers	117:161	the current popular tandem mass spectrometers	117:161	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	6	68	with	1525 N-glycans	1279:1292	arg1	information					1332:1342	comprehensive primary structural information	1299:1342	comprehensive primary structural information (composition, sequence and linkage)	1299:1378	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	69	theme	hits	1258:1261	arg1	NoBHs					1264:1268	NoBHs	1264:1268	NoBHs	1264:1268	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	69	theme	hits	1258:1261	arg1	number					1243:1248	number	1243:1248	number of best hits (NoBHs)	1243:1269	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	69	theme	hits	1258:1261	arg1	hits					1258:1261	best hits	1253:1261	best hits (NoBHs)	1253:1269	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	6	69	theme	hits	1258:1261	arg1	%					1237:1237	FDR ≤1%	1231:1237	FDR ≤1%	1231:1237	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	70	with	identification	1743:1756	arg1	interpretation					1808:1821	comprehensive interpretation	1794:1821	comprehensive interpretation of tandem mass spectra and FDR control	1794:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	3	71	used	used	536:539	arg2	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	71	used	used	536:539	arg2	algorithm					497:505	envelope fingerprinting algorithm	473:505	envelope fingerprinting algorithm	473:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	71	used	used	536:539	arg2	ratio					463:467	The isotopic mass-to-charge ratio	435:467	The isotopic mass-to-charge ratio	435:467	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	7	72	theme	mass	1619:1622	arg1	spectra					1624:1630	tandem mass spectra	1612:1630	tandem mass spectra	1612:1630	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	4	73	theme	2D	812:813	arg1	graph					815:819	pseudo 2D graph	805:819	pseudo 2D graph	805:819	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	2	74	theme	N-glycans	405:413	arg1	Identification					363:376	Identification	363:376	Identification	363:376	Identification and visualization tools of N-glycans are needed.
29105226	2	74	theme	N-glycans	405:413	arg1	tools					396:400	visualization tools	382:400	visualization tools	382:400	Identification and visualization tools of N-glycans are needed.
29105226	5	75	theme	discovery	1019:1027	arg1	FDR					1035:1037	FDR	1035:1037	FDR	1035:1037	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	5	75	theme	discovery	1019:1027	arg1	rate					1029:1032	false discovery rate	1013:1032	false discovery rate (FDR) control	1013:1046	N-glycans were identified with comprehensive interpretation of product ions and false discovery rate (FDR) control.
29105226	6	76	theme	datasets	1156:1163	arg1	search					1071:1076	a database search	1060:1076	a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1060:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	77	theme	mass-to-charge	448:461	arg1	ratio					463:467	The isotopic mass-to-charge ratio	435:467	The isotopic mass-to-charge ratio	435:467	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	77	theme	mass-to-charge	448:461	arg1	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	1	78	dep	structures	208:217	arg1	linkage					261:267	linkage	261:267	linkage	261:267	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	78	dep	structures	208:217	arg1	sequence					248:255	sequence	248:255	sequence	248:255	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	78	dep	structures	208:217	arg1	structures					208:217	the primary structures	196:217	the primary structures (monosaccharide composition, sequence and linkage) of N-glycans	196:281	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	1	78	dep	structures	208:217	arg1	composition					235:245	monosaccharide composition	220:245	monosaccharide composition	220:245	RATIONALE Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far.
29105226	2	79	theme	visualization	382:394	arg1	tools					396:400	visualization tools	382:400	visualization tools	382:400	Identification and visualization tools of N-glycans are needed.
29105226	0	80	theme	N-glycans	48:56	arg1	visualization					31:43	visualization	31:43	visualization	31:43	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	0	80	theme	N-glycans	48:56	arg1	identification					12:25	identification	12:25	identification	12:25	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	7	81	theme	tandem	1612:1617	arg1	spectra					1624:1630	tandem mass spectra	1612:1630	tandem mass spectra	1612:1630	This represents a large-scale identification and visualization of N-glycans with primary structures from tandem mass spectra.
29105226	6	82	theme	spectrometry	1130:1141	arg1	datasets					1156:1163	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets	1081:1163	reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells	1081:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	83	theme	stand-alone	1647:1657	arg1	engine					1684:1689	A stand-alone N-glycan database search engine	1645:1689	A stand-alone N-glycan database search engine called GlySeeker	1645:1706	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	3	84	theme	database	665:672	arg1	engine					681:686	a stand-alone N-glycan database search engine	642:686	a stand-alone N-glycan database search engine	642:686	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	84	theme	database	665:672	arg1	GlySeeker					689:697	GlySeeker	689:697	GlySeeker	689:697	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	0	85	with	visualization	31:43	arg1	structures					71:80	primary structures	63:80	primary structures using GlySeeker	63:96	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	3	86	theme	fingerprinting	482:495	arg1	METHODS					427:433	METHODS	427:433	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm	427:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	3	86	theme	fingerprinting	482:495	arg1	algorithm					497:505	envelope fingerprinting algorithm	473:505	envelope fingerprinting algorithm	473:505	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	0	87	theme	primary	63:69	arg1	structures					71:80	primary structures	63:80	primary structures using GlySeeker	63:96	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	6	88	dep	structures	1470:1479	arg1	i.e.					1459:1462	i.e.	1459:1462	i.e.	1459:1462	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	8	89	theme	database	1668:1675	arg1	engine					1684:1689	A stand-alone N-glycan database search engine	1645:1689	A stand-alone N-glycan database search engine called GlySeeker	1645:1706	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	0	90	with	identification	12:25	arg1	structures					71:80	primary structures	63:80	primary structures using GlySeeker	63:96	Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.
29105226	6	91	theme	cancer	1212:1217	arg1	cells					1219:1223	OVCAR-3 ovarian cancer cells	1196:1223	OVCAR-3 ovarian cancer cells	1196:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	4	92	theme	text	834:837	arg1	formats					839:845	Both pseudo 2D graph and one-line text formats	800:845	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides	800:889	Both pseudo 2D graph and one-line text formats with one-letter symbols for monosaccharides were proposed for representing N-glycans.
29105226	6	93	theme	OVCAR-3	1196:1202	arg1	cells					1219:1223	OVCAR-3 ovarian cancer cells	1196:1223	OVCAR-3 ovarian cancer cells	1196:1223	RESULTS In a database search of reversed-phase liquid chromatography/tandem mass spectrometry (RPLC/MS/MS) datasets of the N-glycome enriched from OVCAR-3 ovarian cancer cells, with FDR ≤1% and number of best hits (NoBHs) = 1-30, 1525 N-glycans with comprehensive primary structural information (composition, sequence and linkage) were identified and visualized; among these 1525 N-glycans, 559 had NoBHs = 1, i.e. their structures were uniquely identified.
29105226	3	94	theme	protein	552:558	arg1	search					569:574	intact protein database search	545:574	intact protein database search	545:574	METHODS The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and identification, was adapted for N-glycan database search, and a stand-alone N-glycan database search engine, GlySeeker, for automated N-glycan identification and visualization was developed and successfully benchmarked.
29105226	8	95	theme	N-glycans	1779:1787	arg1	visualization					1762:1774	visualization	1762:1774	visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control	1762:1860	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
29105226	8	95	theme	N-glycans	1779:1787	arg1	identification					1743:1756	large-scale identification	1731:1756	large-scale identification	1731:1756	CONCLUSIONS A stand-alone N-glycan database search engine called GlySeeker has been developed for large-scale identification and visualization of N-glycans with comprehensive interpretation of tandem mass spectra and FDR control.
31030023	10	0	theme	cell	1498:1501	arg1	viability					1503:1511	good cell viability	1493:1511	good cell viability	1493:1511	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	8	1	theme	deposited	1168:1176	arg1	crystals					1186:1193	The deposited apatite crystals	1164:1193	The deposited apatite crystals on scaffold with 2% MMT content	1164:1225	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	1	2	theme	scaffold	173:180	arg1	fabrication					111:121	the fabrication	107:121	the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications	107:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	7	3	theme	apatite	1094:1100	arg1	needles					1102:1108	apatite needles	1094:1108	apatite needles	1094:1108	The average length and thickness of apatite needles were calculated to be 140 μm and 1.2 μm respectively.
31030023	1	4	theme	tissue	262:267	arg1	applications					281:292	bone tissue engineering applications	257:292	bone tissue engineering applications	257:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	5	5	theme	polymer	812:818	arg1	matrix					820:825	polymer matrix	812:825	polymer matrix	812:825	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	9	6	theme	scaffold	1341:1348	arg1	Swelling					1301:1308	Swelling	1301:1308	Swelling	1301:1308	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	9	6	theme	scaffold	1341:1348	arg1	behavior					1329:1336	biodegradation behavior	1314:1336	biodegradation behavior	1314:1336	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	3	7	with	porous	516:521	arg1	variations					528:537	variations	528:537	variations in pore size (102 μm-290 μm)	528:566	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	10	8	theme	MTT	1436:1438	arg1	assay					1440:1444	MTT assay	1436:1444	MTT assay	1436:1444	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	8	9	theme	natural	1279:1285	arg1	apatite					1292:1298	natural bone apatite	1279:1298	natural bone apatite	1279:1298	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	1	10	theme	engineering	269:279	arg1	applications					281:292	bone tissue engineering applications	257:292	bone tissue engineering applications	257:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	5	11	theme	bio-mineralization	744:761	arg1	study					763:767	In-vitro bio-mineralization study	735:767	In-vitro bio-mineralization study	735:767	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	0	12	from	Effect	0:5	arg1	scaffold					71:78	Xylan/Chitosan conjugate scaffold	46:78	Xylan/Chitosan conjugate scaffold	46:78	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.
31030023	9	13	from	effect	1407:1412	arg1	composites					1424:1433	composites	1424:1433	composites	1424:1433	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	6	14	theme	MMT	846:848	arg1	concentration					850:862	5% MMT concentration	843:862	5% MMT concentration	843:862	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	4	15	theme	polymer	668:674	arg1	Xylan/Chitosan					688:701	Xylan/Chitosan	688:701	Xylan/Chitosan	688:701	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	4	15	theme	polymer	668:674	arg1	conjugates					676:685	polymer conjugates	668:685	polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction	668:732	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	6	16	theme	body	1046:1049	arg1	fluid					1051:1055	body fluid	1046:1055	body fluid	1046:1055	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	8	17	theme	2	1212:1212	arg1	%					1213:1213	%	1213:1213	%	1213:1213	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	6	18	theme	synergistic	944:954	arg1	response					956:963	synergistic response	944:963	synergistic response	944:963	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	10	19	theme	scaffold	1479:1486	arg1	nature					1469:1474	non-cytotoxic nature	1455:1474	non-cytotoxic nature of scaffold with good cell viability	1455:1511	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	6	20	theme	%	844:844	arg1	concentration					850:862	5% MMT concentration	843:862	5% MMT concentration	843:862	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	2	21	theme	X-ray	383:387	arg1	XRD					402:404	XRD	402:404	XRD	402:404	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	2	21	theme	X-ray	383:387	arg1	diffraction					389:399	X-ray diffraction	383:399	X-ray diffraction (XRD)	383:405	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	2	22	dep	transform	342:350	arg1	infrared					352:359	infrared	352:359	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing	342:464	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	6	23	with	Scaffolds	828:836	arg1	concentration					850:862	5% MMT concentration	843:862	5% MMT concentration	843:862	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	3	24	from	variations	528:537	arg1	size					547:550	pore size	542:550	pore size (102 μm-290 μm)	542:566	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	3	24	from	variations	528:537	arg1	102 μm-290 μm					553:565	102 μm-290 μm	553:565	102 μm-290 μm	553:565	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	6	25	with	contact	1033:1039	arg1	fluid					1051:1055	body fluid	1046:1055	body fluid	1046:1055	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	1	26	theme	freeze	185:190	arg1	process					199:205	freeze drying process	185:205	freeze drying process with the aim of achieving improved properties for bone tissue engineering applications	185:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	5	27	theme	significant	778:788	arg1	deposition					798:807	significant apatite deposition	778:807	significant apatite deposition on polymer matrix	778:825	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	9	28	theme	biodegradation	1314:1327	arg1	behavior					1329:1336	biodegradation behavior	1314:1336	biodegradation behavior	1314:1336	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	1	29	theme	present	85:91	arg1	study					93:97	The present study	81:97	The present study	81:97	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	7	30	dep	length	1070:1075	arg1	The					1058:1060	The	1058:1060	The	1058:1060	The average length and thickness of apatite needles were calculated to be 140 μm and 1.2 μm respectively.
31030023	1	31	theme	drying	192:197	arg1	process					199:205	freeze drying process	185:205	freeze drying process with the aim of achieving improved properties for bone tissue engineering applications	185:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	8	32	theme	Ca/P	1240:1243	arg1	ratio					1245:1249	Ca/P ratio	1240:1249	Ca/P ratio	1240:1249	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	9	33	theme	barrier	1399:1405	arg1	effect					1407:1412	barrier effect	1399:1412	barrier effect of MMT on composites	1399:1433	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	2	34	theme	mechanical	447:456	arg1	testing					458:464	mechanical testing	447:464	mechanical testing	447:464	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	5	35	from	deposition	798:807	arg1	matrix					820:825	polymer matrix	812:825	polymer matrix	812:825	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	10	36	theme	non-cytotoxic	1455:1467	arg1	nature					1469:1474	non-cytotoxic nature	1455:1474	non-cytotoxic nature of scaffold with good cell viability	1455:1511	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	8	37	theme	bone	1287:1290	arg1	apatite					1292:1298	natural bone apatite	1279:1298	natural bone apatite	1279:1298	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	8	38	theme	MMT	1215:1217	arg1	content					1219:1225	2% MMT content	1212:1225	2% MMT content	1212:1225	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	6	39	theme	5	843:843	arg1	%					844:844	%	844:844	%	844:844	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	8	40	with	crystals	1186:1193	arg1	content					1219:1225	2% MMT content	1212:1225	2% MMT content	1212:1225	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	3	41	theme	fabricated	471:480	arg1	porous					516:521	porous	516:521	porous	516:521	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	3	41	theme	fabricated	471:480	arg1	scaffolds					482:490	The fabricated scaffolds	467:490	The fabricated scaffolds	467:490	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	0	42	theme	incorporation	10:22	arg1	Effect					0:5	Effect	0:5	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.	0:79	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.
31030023	4	43	theme	Maillard	716:723	arg1	reaction					725:732	Maillard reaction	716:732	Maillard reaction	716:732	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	6	44	theme	apatite	910:916	arg1	morphology					886:895	needle like morphology	874:895	needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds	874:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	5	45	theme	In-vitro	735:742	arg1	study					763:767	In-vitro bio-mineralization study	735:767	In-vitro bio-mineralization study	735:767	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	2	46	theme	Scanning	408:415	arg1	microscopy					426:435	Scanning electron microscopy	408:435	Scanning electron microscopy (SEM)	408:441	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	2	46	theme	Scanning	408:415	arg1	SEM					438:440	SEM	438:440	SEM	438:440	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	6	47	theme	scaffolds	1008:1016	arg1	properties					994:1003	the mechanical properties	979:1003	the mechanical properties of scaffolds	979:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	0	48	theme	montmorillonite	27:41	arg1	incorporation					10:22	incorporation	10:22	incorporation of montmorillonite	10:41	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.
31030023	6	49	theme	deposited	900:908	arg1	apatite					910:916	deposited apatite	900:916	deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds	900:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	2	50	theme	electron	417:424	arg1	microscopy					426:435	Scanning electron microscopy	408:435	Scanning electron microscopy (SEM)	408:441	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	2	50	theme	electron	417:424	arg1	SEM					438:440	SEM	438:440	SEM	438:440	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	0	51	theme	conjugate	61:69	arg1	scaffold					71:78	Xylan/Chitosan conjugate scaffold	46:78	Xylan/Chitosan conjugate scaffold	46:78	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.
31030023	8	52	theme	%	1213:1213	arg1	content					1219:1225	2% MMT content	1212:1225	2% MMT content	1212:1225	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	1	53	with	process	199:205	arg1	aim					216:218	the aim	212:218	the aim of achieving improved properties for bone tissue engineering applications	212:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	8	54	from	crystals	1186:1193	arg1	scaffold					1198:1205	scaffold	1198:1205	scaffold	1198:1205	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	10	55	theme	good	1493:1496	arg1	viability					1503:1511	good cell viability	1493:1511	good cell viability	1493:1511	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	2	56	dep	Fourier	334:340	arg1	transform					342:350	transform	342:350	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing	342:464	The scaffolds were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and mechanical testing.
31030023	0	57	theme	Xylan/Chitosan	46:59	arg1	scaffold					71:78	Xylan/Chitosan conjugate scaffold	46:78	Xylan/Chitosan conjugate scaffold	46:78	Effect of incorporation of montmorillonite on Xylan/Chitosan conjugate scaffold.
31030023	9	58	theme	MMT	1417:1419	arg1	effect					1407:1412	barrier effect	1399:1412	barrier effect of MMT on composites	1399:1433	Swelling and biodegradation behavior of scaffold were also studied with regard to hydrophilic and barrier effect of MMT on composites.
31030023	7	59	theme	needles	1102:1108	arg1	thickness					1081:1089	thickness	1081:1089	thickness	1081:1089	The average length and thickness of apatite needles were calculated to be 140 μm and 1.2 μm respectively.
31030023	7	59	theme	needles	1102:1108	arg1	length					1070:1075	average length	1062:1075	average length	1062:1075	The average length and thickness of apatite needles were calculated to be 140 μm and 1.2 μm respectively.
31030023	3	60	theme	filler	583:588	arg1	concentration					590:602	the filler concentration	579:602	the filler concentration	579:602	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	8	61	theme	1.67	1254:1257	arg1	ratio					1245:1249	Ca/P ratio	1240:1249	Ca/P ratio	1240:1249	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	1	62	theme	Xylan/Chitosan/Montmorillonite	126:155	arg1	scaffold					173:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	6	63	theme	mechanical	983:992	arg1	properties					994:1003	the mechanical properties	979:1003	the mechanical properties of scaffolds	979:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	8	64	theme	apatite	1178:1184	arg1	crystals					1186:1193	The deposited apatite crystals	1164:1193	The deposited apatite crystals on scaffold with 2% MMT content	1164:1225	The deposited apatite crystals on scaffold with 2% MMT content demonstrated Ca/P ratio of 1.67, resembling that of natural bone apatite.
31030023	6	65	theme	like	881:884	arg1	morphology					886:895	needle like morphology	874:895	needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds	874:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	7	66	theme	average	1062:1068	arg1	length					1070:1075	average length	1062:1075	average length	1062:1075	The average length and thickness of apatite needles were calculated to be 140 μm and 1.2 μm respectively.
31030023	1	67	theme	improved	233:240	arg1	properties					242:251	improved properties	233:251	improved properties for bone tissue engineering applications	233:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	3	68	theme	pore	542:545	arg1	size					547:550	pore size	542:550	pore size (102 μm-290 μm)	542:566	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	3	68	theme	pore	542:545	arg1	102 μm-290 μm					553:565	102 μm-290 μm	553:565	102 μm-290 μm	553:565	The fabricated scaffolds were found to be highly porous with variations in pore size (102 μm-290 μm) on varying the filler concentration.
31030023	6	69	theme	needle	874:879	arg1	morphology					886:895	needle like morphology	874:895	needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds	874:1016	Scaffolds with 5% MMT concentration exhibited needle like morphology of deposited apatite which can further provide synergistic response in increasing the mechanical properties of scaffolds when placed in contact with body fluid.
31030023	1	70	theme	MMT	158:160	arg1	scaffold					173:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	4	71	theme	XRD	605:607	arg1	study					609:613	XRD study	605:613	XRD study	605:613	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	5	72	theme	apatite	790:796	arg1	deposition					798:807	significant apatite deposition	778:807	significant apatite deposition on polymer matrix	778:825	In-vitro bio-mineralization study revealed significant apatite deposition on polymer matrix.
31030023	4	73	theme	MMT	648:650	arg1	exfoliation					633:643	complete exfoliation	624:643	complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction	624:732	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	4	74	theme	complete	624:631	arg1	exfoliation					633:643	complete exfoliation	624:643	complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction	624:732	XRD study revealed complete exfoliation of MMT incorporated in polymer conjugates (Xylan/Chitosan) prepared by Maillard reaction.
31030023	10	75	with	nature	1469:1474	arg1	viability					1503:1511	good cell viability	1493:1511	good cell viability	1493:1511	MTT assay revealed non-cytotoxic nature of scaffold with good cell viability.
31030023	1	76	theme	composite	163:171	arg1	scaffold					173:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	Xylan/Chitosan/Montmorillonite (MMT) composite scaffold	126:180	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
31030023	1	77	theme	bone	257:260	arg1	applications					281:292	bone tissue engineering applications	257:292	bone tissue engineering applications	257:292	The present study reports the fabrication of Xylan/Chitosan/Montmorillonite (MMT) composite scaffold by freeze drying process with the aim of achieving improved properties for bone tissue engineering applications.
30366288	0	0	theme	polysaccharide	74:87	arg1	degradability					89:101	enzymatic cell wall polysaccharide degradability	54:101	enzymatic cell wall polysaccharide degradability	54:101	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	5	1	theme	lowering	1065:1072	arg1	concentration					1082:1094	lowering ammonia concentration	1065:1094	lowering ammonia concentration	1065:1094	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	5	2	from	effect	1110:1115	arg1	degradability					1152:1164	the enzymatic degradability	1138:1164	the enzymatic degradability of the residual fraction	1138:1189	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	1	3	theme	lignocellulose	170:183	arg1	treatment					157:165	Ammonia treatment	149:165	Ammonia treatment of lignocellulose	149:183	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	3	4	theme	material	787:794	arg1	glucan					765:770	glucan	765:770	glucan of the treated material	765:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	0	5	theme	wall	69:72	arg1	degradability					89:101	enzymatic cell wall polysaccharide degradability	54:101	enzymatic cell wall polysaccharide degradability	54:101	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	3	6	theme	carbohydrate	680:691	arg1	recovery					693:700	a high carbohydrate recovery	673:700	a high carbohydrate recovery (>80%)	673:707	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	6	theme	carbohydrate	680:691	arg1	%					706:706	>80%	703:706	>80%	703:706	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	7	theme	statistical	372:382	arg1	design					384:389	a statistical design	370:389	a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions	370:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	8	theme	cellulase	812:820	arg1	cocktail					829:836	a (hemi-) cellulase enzyme cocktail	802:836	a (hemi-) cellulase enzyme cocktail	802:836	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	5	9	theme	time	1130:1133	arg1	effect					1110:1115	the effect	1106:1115	the effect of treatment time on the enzymatic degradability of the residual fraction	1106:1189	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	2	10	theme	low	325:327	arg1	treatment					342:350	low dose ammonia treatment	325:350	low dose ammonia treatment of wheat straw	325:365	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	11	theme	enzyme	822:827	arg1	cocktail					829:836	a (hemi-) cellulase enzyme cocktail	802:836	a (hemi-) cellulase enzyme cocktail	802:836	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	4	12	theme	coumaric	918:925	arg1	acid					927:930	coumaric acid	918:930	coumaric acid	918:930	This effect coincidences with the relative decrease in ferulic acid by 10% and coumaric acid by more than 50% analysed via pyrolysis-GC-MS, measured as 4-vinyl-phenol and 4-vinyl-guaiacol, respectively.
30366288	4	13	theme	ferulic	894:900	arg1	acid					902:905	ferulic acid	894:905	ferulic acid	894:905	This effect coincidences with the relative decrease in ferulic acid by 10% and coumaric acid by more than 50% analysed via pyrolysis-GC-MS, measured as 4-vinyl-phenol and 4-vinyl-guaiacol, respectively.
30366288	2	14	theme	wheat	355:359	arg1	straw					361:365	wheat straw	355:365	wheat straw	355:365	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	0	15	theme	residual	117:124	arg1	acids					142:146	residual hydroxycinnamic acids	117:146	residual hydroxycinnamic acids	117:146	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	3	16	theme	enzymatic	717:725	arg1	hydrolysis					727:736	enzymatic hydrolysis	717:736	enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material	717:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	1	17	theme	carbohydrate	194:205	arg1	degradability					207:219	carbohydrate degradability	194:219	carbohydrate degradability	194:219	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	0	18	theme	straw	38:42	arg1	treatment					19:27	Low liquid ammonia treatment	0:27	Low liquid ammonia treatment of wheat straw	0:42	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	3	19	dep	resulted	661:668	arg1	coupled					709:715	coupled	709:715	coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail	709:836	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	20	theme	concentration	458:470	arg1	effects					439:445	the effects	435:445	the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions	435:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	5	21	theme	residual	1173:1180	arg1	fraction					1182:1189	the residual fraction	1169:1189	the residual fraction	1169:1189	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	0	22	theme	liquid	4:9	arg1	treatment					19:27	Low liquid ammonia treatment	0:27	Low liquid ammonia treatment of wheat straw	0:42	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	2	23	theme	Solid	496:500	arg1	ratio					509:513	the Solid:Liquid ratio	492:513	the Solid:Liquid ratio	492:513	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	24	theme	ratio	509:513	arg1	effects					439:445	the effects	435:445	the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions	435:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	25	dep	Solid	496:500	arg1	Liquid					502:507	Liquid	502:507	Liquid	502:507	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	26	theme	Taguchi	407:413	arg1	design					415:420	Taguchi design	407:420	Taguchi design	407:420	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	26	theme	Taguchi	407:413	arg1	experiments					394:404	experiments	394:404	experiments (Taguchi design)	394:421	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	0	27	theme	Low	0:2	arg1	treatment					19:27	Low liquid ammonia treatment	0:27	Low liquid ammonia treatment of wheat straw	0:42	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	5	28	theme	treatment	1120:1128	arg1	time					1130:1133	treatment time	1120:1133	treatment time	1120:1133	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	2	29	theme	ammonia	450:456	arg1	concentration					458:470	ammonia concentration	450:470	ammonia concentration	450:470	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	30	theme	%	743:743	arg1	hydrolysis					727:736	enzymatic hydrolysis	717:736	enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material	717:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	4	31	theme	relative	873:880	arg1	decrease					882:889	the relative decrease	869:889	the relative decrease in ferulic acid	869:905	This effect coincidences with the relative decrease in ferulic acid by 10% and coumaric acid by more than 50% analysed via pyrolysis-GC-MS, measured as 4-vinyl-phenol and 4-vinyl-guaiacol, respectively.
30366288	2	32	theme	ammonia	334:340	arg1	treatment					342:350	low dose ammonia treatment	325:350	low dose ammonia treatment of wheat straw	325:365	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	0	33	theme	ammonia	11:17	arg1	treatment					19:27	Low liquid ammonia treatment	0:27	Low liquid ammonia treatment of wheat straw	0:42	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	0	34	theme	hydroxycinnamic	126:140	arg1	acids					142:146	residual hydroxycinnamic acids	117:146	residual hydroxycinnamic acids	117:146	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	2	35	theme	dose	329:332	arg1	treatment					342:350	low dose ammonia treatment	325:350	low dose ammonia treatment of wheat straw	325:365	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	1	36	theme	low	231:233	arg1	effects					258:264	low ammonia dose treatment effects	231:264	low ammonia dose treatment effects	231:264	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	0	37	theme	wheat	32:36	arg1	straw					38:42	wheat straw	32:42	wheat straw	32:42	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	2	38	theme	enzymatic	545:553	arg1	degradability					555:567	enzymatic degradability	545:567	enzymatic degradability	545:567	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	4	39	from	decrease	882:889	arg1	acid					902:905	ferulic acid	894:905	ferulic acid	894:905	This effect coincidences with the relative decrease in ferulic acid by 10% and coumaric acid by more than 50% analysed via pyrolysis-GC-MS, measured as 4-vinyl-phenol and 4-vinyl-guaiacol, respectively.
30366288	1	40	theme	ammonia	235:241	arg1	effects					258:264	low ammonia dose treatment effects	231:264	low ammonia dose treatment effects	231:264	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	3	41	theme	treated	779:785	arg1	material					787:794	the treated material	775:794	the treated material	775:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	42	theme	≤2 w/w 	647:653	arg1	NH3					656:658	≤2 w/w % NH3	647:658	≤2 w/w % NH3	647:658	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	42	theme	≤2 w/w 	647:653	arg1	concentration					632:644	low ammonia concentration	620:644	low ammonia concentration (≤2 w/w % NH3)	620:659	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	1	43	theme	dose	243:246	arg1	effects					258:264	low ammonia dose treatment effects	231:264	low ammonia dose treatment effects	231:264	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	2	44	theme	time	483:486	arg1	effects					439:445	the effects	435:445	the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions	435:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	45	theme	%	654:654	arg1	NH3					656:658	≤2 w/w % NH3	647:658	≤2 w/w % NH3	647:658	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	45	theme	%	654:654	arg1	concentration					632:644	low ammonia concentration	620:644	low ammonia concentration (≤2 w/w % NH3)	620:659	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	5	46	theme	ammonia	1074:1080	arg1	concentration					1082:1094	lowering ammonia concentration	1065:1094	lowering ammonia concentration	1065:1094	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	2	47	theme	treatment	473:481	arg1	time					483:486	treatment time	473:486	treatment time	473:486	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	48	theme	fractions	585:593	arg1	degradability					555:567	enzymatic degradability	545:567	enzymatic degradability	545:567	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	48	theme	fractions	585:593	arg1	structure					518:526	structure	518:526	structure	518:526	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	48	theme	fractions	585:593	arg1	composition					529:539	composition	529:539	composition	529:539	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	49	theme	hemi-	805:809	arg1	cocktail					829:836	a (hemi-) cellulase enzyme cocktail	802:836	a (hemi-) cellulase enzyme cocktail	802:836	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	50	theme	xylan	748:752	arg1	glucan					765:770	glucan	765:770	glucan of the treated material	765:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	50	theme	xylan	748:752	arg1	xylan					748:752	xylan	748:752	xylan	748:752	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	50	theme	xylan	748:752	arg1	%					760:760	40%	758:760	40% of glucan of the treated material	758:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	50	theme	xylan	748:752	arg1	%					743:743	50%	741:743	50% of xylan	741:752	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	51	theme	residual	576:583	arg1	fractions					585:593	the residual fractions	572:593	the residual fractions	572:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	52	theme	high	675:678	arg1	recovery					693:700	a high carbohydrate recovery	673:700	a high carbohydrate recovery (>80%)	673:707	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	52	theme	high	675:678	arg1	%					706:706	>80%	703:706	>80%	703:706	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	53	theme	straw	361:365	arg1	treatment					342:350	low dose ammonia treatment	325:350	low dose ammonia treatment of wheat straw	325:365	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	1	54	theme	treatment	248:256	arg1	effects					258:264	low ammonia dose treatment effects	231:264	low ammonia dose treatment effects	231:264	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	5	55	theme	enzymatic	1142:1150	arg1	degradability					1152:1164	the enzymatic degradability	1138:1164	the enzymatic degradability of the residual fraction	1138:1189	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	1	56	theme	Ammonia	149:155	arg1	treatment					157:165	Ammonia treatment	149:165	Ammonia treatment of lignocellulose	149:183	Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered.
30366288	5	57	theme	fraction	1182:1189	arg1	degradability					1152:1164	the enzymatic degradability	1138:1164	the enzymatic degradability of the residual fraction	1138:1189	Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.
30366288	0	58	theme	cell	64:67	arg1	degradability					89:101	enzymatic cell wall polysaccharide degradability	54:101	enzymatic cell wall polysaccharide degradability	54:101	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	3	59	theme	low	620:622	arg1	NH3					656:658	≤2 w/w % NH3	647:658	≤2 w/w % NH3	647:658	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	59	theme	low	620:622	arg1	concentration					632:644	low ammonia concentration	620:644	low ammonia concentration (≤2 w/w % NH3)	620:659	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	60	theme	experiments	394:404	arg1	design					384:389	a statistical design	370:389	a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions	370:593	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	61	theme	%	760:760	arg1	hydrolysis					727:736	enzymatic hydrolysis	717:736	enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material	717:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	0	62	theme	enzymatic	54:62	arg1	degradability					89:101	enzymatic cell wall polysaccharide degradability	54:101	enzymatic cell wall polysaccharide degradability	54:101	Low liquid ammonia treatment of wheat straw increased enzymatic cell wall polysaccharide degradability and decreased residual hydroxycinnamic acids.
30366288	3	63	theme	ammonia	624:630	arg1	NH3					656:658	≤2 w/w % NH3	647:658	≤2 w/w % NH3	647:658	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	63	theme	ammonia	624:630	arg1	concentration					632:644	low ammonia concentration	620:644	low ammonia concentration (≤2 w/w % NH3)	620:659	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	2	64	from	effects	439:445	arg1	degradability					555:567	enzymatic degradability	545:567	enzymatic degradability	545:567	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	64	from	effects	439:445	arg1	structure					518:526	structure	518:526	structure	518:526	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	2	64	from	effects	439:445	arg1	composition					529:539	composition	529:539	composition	529:539	This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions.
30366288	3	65	theme	glucan	765:770	arg1	glucan					765:770	glucan	765:770	glucan of the treated material	765:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	65	theme	glucan	765:770	arg1	xylan					748:752	xylan	748:752	xylan	748:752	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	65	theme	glucan	765:770	arg1	%					760:760	40%	758:760	40% of glucan of the treated material	758:794	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
30366288	3	65	theme	glucan	765:770	arg1	%					743:743	50%	741:743	50% of xylan	741:752	The results showed that low ammonia concentration (≤2 w/w % NH3) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail.
31271800	0	0	theme	polysaccharides	92:106	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	0	theme	polysaccharides	92:106	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	0	theme	polysaccharides	92:106	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	0	theme	polysaccharides	92:106	arg1	characterization					25:40	characterization	25:40	characterization	25:40	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	1	from	purification	11:22	arg1	latifolia					123:131	Sparassis latifolia	113:131	Sparassis latifolia	113:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	5	2	contain	had	859:861	arg1	polysaccharides					758:772	The polysaccharides	754:772	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3)	754:857	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	2	contain	had	859:861	arg2	activities					882:891	higher antioxidant activities	863:891	higher antioxidant activities	863:891	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	3	theme	antioxidant	870:880	arg1	activities					882:891	higher antioxidant activities	863:891	higher antioxidant activities	863:891	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	0	4	theme	low-molecular-weight	71:90	arg1	polysaccharides					92:106	low-molecular-weight polysaccharides	71:106	low-molecular-weight polysaccharides from Sparassis latifolia	71:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	8	5	theme	polysaccharides	1304:1318	arg1	source					1243:1248	source	1243:1248	source	1243:1248	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	8	5	theme	polysaccharides	1304:1318	arg1	size					1214:1217	size	1214:1217	size	1214:1217	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	8	5	theme	polysaccharides	1304:1318	arg1	method					1231:1236	extraction method	1220:1236	extraction method	1220:1236	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	6	6	theme	polysaccharides	987:1001	arg1	yield					947:951	extraction yield	936:951	extraction yield	936:951	Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed.
31271800	6	6	theme	polysaccharides	987:1001	arg1	activities					969:978	antioxidant activities	957:978	antioxidant activities	957:978	Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed.
31271800	0	7	from	Isolation	0:8	arg1	latifolia					123:131	Sparassis latifolia	113:131	Sparassis latifolia	113:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	9	8	contain	have	1392:1395	arg2	activities					1416:1425	strong antioxidant activities	1397:1425	strong antioxidant activities closely associated with the polysaccharide structure and extraction method	1397:1500	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	8	contain	have	1392:1395	arg1	polysaccharides					1349:1363	polysaccharides	1349:1363	polysaccharides isolated from S. latifolia	1349:1390	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	9	theme	polysaccharides	1562:1576	arg1	use					1549:1551	further use	1541:1551	further use of these polysaccharides	1541:1576	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	2	10	theme	branching	484:492	arg1	degree					474:479	a degree	472:479	a degree of branching (DB) ranging from 0.2 to 0.4	472:521	Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4.
31271800	9	11	theme	strong	1397:1402	arg1	activities					1416:1425	strong antioxidant activities	1397:1425	strong antioxidant activities closely associated with the polysaccharide structure and extraction method	1397:1500	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	7	12	theme	antioxidant	1099:1109	arg1	activities					1111:1120	antioxidant activities	1099:1120	antioxidant activities	1099:1120	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	8	13	theme	fermentation	1251:1262	arg1	liquor					1264:1269	fermentation liquor	1251:1269	fermentation liquor	1251:1269	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	1	14	theme	Sparassis	332:340	arg1	mycelia					304:310	mycelia	304:310	mycelia	304:310	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	14	theme	Sparassis	332:340	arg1	basidiomata					317:327	basidiomata	317:327	basidiomata	317:327	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	14	theme	Sparassis	332:340	arg1	liquor					296:301	the fermentation liquor	279:301	the fermentation liquor	279:301	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	15	dep	polysaccharides	138:152	arg1	SF-FF51					191:197	SF-FF51	191:197	SF-FF51	191:197	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	15	dep	polysaccharides	138:152	arg1	SF-FR61					204:210	SF-FR61	204:210	SF-FR61	204:210	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	15	dep	polysaccharides	138:152	arg1	polysaccharides					138:152	Six polysaccharides	134:152	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa)	134:257	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	4	16	theme	antioxidant	646:656	arg1	activities					658:667	Their antioxidant activities	640:667	Their antioxidant activities	640:667	Their antioxidant activities were correlated to some extent with their composition but significantly with the DB.
31271800	5	17	theme	glucose	811:817	arg1	ratio					830:834	glucose: galactose ratio	811:834	glucose: galactose ratio	811:834	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	0	18	from	characterization	25:40	arg1	latifolia					123:131	Sparassis latifolia	113:131	Sparassis latifolia	113:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	8	19	theme	antioxidant	1156:1166	arg1	activities					1168:1177	antioxidant activities	1156:1177	antioxidant activities	1156:1177	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	7	20	contain	had	1075:1077	arg2	yield					1089:1093	high yield	1084:1093	high yield	1084:1093	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	7	20	contain	had	1075:1077	arg1	polysaccharides					1028:1042	The low-MW polysaccharides	1017:1042	The low-MW polysaccharides extracted by alkali treatments	1017:1073	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	7	20	contain	had	1075:1077	arg2	activities					1111:1120	antioxidant activities	1099:1120	antioxidant activities	1099:1120	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	9	21	theme	antioxidant	1404:1414	arg1	activities					1416:1425	strong antioxidant activities	1397:1425	strong antioxidant activities closely associated with the polysaccharide structure and extraction method	1397:1500	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	2	22	theme	polysaccharides	412:426	arg1	analyses					385:392	Structural analyses	374:392	Structural analyses of these purified polysaccharides	374:426	Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4.
31271800	6	23	theme	antioxidant	957:967	arg1	activities					969:978	antioxidant activities	957:978	antioxidant activities	957:978	Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed.
31271800	9	24	dep	structure	1470:1478	arg1	the					1451:1453	the	1451:1453	the	1451:1453	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	25	theme	extraction	1484:1493	arg1	method					1495:1500	extraction method	1484:1500	extraction method	1484:1500	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	2	26	theme	purified	403:410	arg1	polysaccharides					412:426	these purified polysaccharides	397:426	these purified polysaccharides	397:426	Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4.
31271800	7	27	theme	high	1084:1087	arg1	yield					1089:1093	high yield	1084:1093	high yield	1084:1093	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	5	28	theme	galactose	820:828	arg1	ratio					830:834	glucose: galactose ratio	811:834	glucose: galactose ratio	811:834	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	29	theme	low	781:783	arg1	content					799:805	a low 1,3‑d‑glucose content	779:805	a low 1,3‑d‑glucose content	779:805	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	1	30	theme	different	355:363	arg1	methods					365:371	different methods	355:371	different methods	355:371	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	3	31	theme	strong	554:559	arg1	activities					572:581	strong scavenging activities	554:581	strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals	554:637	The polysaccharides exhibited strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals.
31271800	5	32	theme	1,3‑d‑glucose	785:797	arg1	content					799:805	a low 1,3‑d‑glucose content	779:805	a low 1,3‑d‑glucose content	779:805	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	1	33	dep	Sparassis	332:340	arg1	latifolia					342:350	Sparassis latifolia	332:350	Sparassis latifolia	332:350	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	8	34	dep	size	1214:1217	arg1	basidiomata					1284:1294	basidiomata	1284:1294	basidiomata	1284:1294	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	8	34	dep	size	1214:1217	arg1	liquor					1264:1269	fermentation liquor	1251:1269	fermentation liquor	1251:1269	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	8	34	dep	size	1214:1217	arg1	mycelia					1272:1278	mycelia	1272:1278	mycelia	1272:1278	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	3	35	theme	hydroxyl	621:628	arg1	radicals					630:637	hydroxyl radicals	621:637	hydroxyl radicals	621:637	The polysaccharides exhibited strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals.
31271800	3	36	theme	scavenging	561:570	arg1	activities					572:581	strong scavenging activities	554:581	strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals	554:637	The polysaccharides exhibited strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals.
31271800	2	37	theme	Structural	374:383	arg1	analyses					385:392	Structural analyses	374:392	Structural analyses of these purified polysaccharides	374:426	Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4.
31271800	0	38	theme	antioxidant	47:57	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	6	39	theme	extraction	936:945	arg1	yield					947:951	extraction yield	936:951	extraction yield	936:951	Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed.
31271800	0	40	from	activity	59:66	arg1	latifolia					123:131	Sparassis latifolia	113:131	Sparassis latifolia	113:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	7	41	theme	low-MW	1021:1026	arg1	polysaccharides					1028:1042	The low-MW polysaccharides	1017:1042	The low-MW polysaccharides extracted by alkali treatments	1017:1073	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	8	42	theme	extraction	1220:1229	arg1	method					1231:1236	extraction method	1220:1236	extraction method	1220:1236	Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides.
31271800	1	43	theme	fermentation	283:294	arg1	liquor					296:301	the fermentation liquor	279:301	the fermentation liquor	279:301	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	7	44	theme	alkali	1057:1062	arg1	treatments					1064:1073	alkali treatments	1057:1073	alkali treatments	1057:1073	The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities.
31271800	6	45	dep	yield	947:951	arg1	the					932:934	the	932:934	the	932:934	Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed.
31271800	0	46	from	latifolia	123:131	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	46	from	latifolia	123:131	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	46	from	latifolia	123:131	arg1	polysaccharides					92:106	low-molecular-weight polysaccharides	71:106	low-molecular-weight polysaccharides from Sparassis latifolia	71:131	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	46	from	latifolia	123:131	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	0	46	from	latifolia	123:131	arg1	characterization					25:40	characterization	25:40	characterization	25:40	Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia.
31271800	9	47	theme	scientific	1520:1529	arg1	basis					1531:1535	a scientific basis	1518:1535	a scientific basis for further use of these polysaccharides	1518:1576	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	48	attach	isolated	1365:1372	arg1	latifolia					1382:1390	S. latifolia	1379:1390	S. latifolia	1379:1390	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	48	attach	isolated	1365:1372	arg2	polysaccharides					1349:1363	polysaccharides	1349:1363	polysaccharides isolated from S. latifolia	1349:1390	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	9	49	theme	further	1541:1547	arg1	use					1549:1551	further use	1541:1551	further use of these polysaccharides	1541:1576	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	1	50	theme	similar	216:222	arg1	MW					243:244	MW	243:244	MW	243:244	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	50	theme	similar	216:222	arg1	30-50 kDa					248:256	30-50 kDa	248:256	30-50 kDa	248:256	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	50	theme	similar	216:222	arg1	weights					234:240	similar molecular weights	216:240	similar molecular weights (MW) (30-50 kDa)	216:257	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	2	51	dep	0.4	519:521	arg1	to					516:517	to	516:517	to	516:517	Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4.
31271800	5	52	theme	low	842:844	arg1	0.2-0.3					850:856	0.2-0.3	850:856	0.2-0.3	850:856	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	52	theme	low	842:844	arg1	DB					846:847	a low DB	840:847	a low DB (0.2-0.3)	840:857	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	9	53	theme	polysaccharide	1455:1468	arg1	structure					1470:1478	polysaccharide structure	1455:1478	polysaccharide structure	1455:1478	These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
31271800	1	54	theme	molecular	224:232	arg1	MW					243:244	MW	243:244	MW	243:244	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	54	theme	molecular	224:232	arg1	30-50 kDa					248:256	30-50 kDa	248:256	30-50 kDa	248:256	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	54	theme	molecular	224:232	arg1	weights					234:240	similar molecular weights	216:240	similar molecular weights (MW) (30-50 kDa)	216:257	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	5	55	with	polysaccharides	758:772	arg1	content					799:805	a low 1,3‑d‑glucose content	779:805	a low 1,3‑d‑glucose content	779:805	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	55	with	polysaccharides	758:772	arg1	0.2-0.3					850:856	0.2-0.3	850:856	0.2-0.3	850:856	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	55	with	polysaccharides	758:772	arg1	DB					846:847	a low DB	840:847	a low DB (0.2-0.3)	840:857	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	55	with	polysaccharides	758:772	arg1	ratio					830:834	glucose: galactose ratio	811:834	glucose: galactose ratio	811:834	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	5	56	theme	higher	863:868	arg1	activities					882:891	higher antioxidant activities	863:891	higher antioxidant activities	863:891	The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities.
31271800	1	57	theme	weights	234:240	arg1	SF-FF51					191:197	SF-FF51	191:197	SF-FF51	191:197	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	57	theme	weights	234:240	arg1	SF-FR61					204:210	SF-FR61	204:210	SF-FR61	204:210	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
31271800	1	57	theme	weights	234:240	arg1	polysaccharides					138:152	Six polysaccharides	134:152	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa)	134:257	Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods.
30586626	8	0	theme	close	1211:1215	arg1	contact					1217:1223	close contact	1211:1223	close contact with the known binding residues after about 100 ns simulation time	1211:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	6	1	theme	ATIII	965:969	arg1	structure					971:979	the previously liganded ATIII structure	941:979	the previously liganded ATIII structure	941:979	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	6	1	theme	ATIII	965:969	arg1	input					993:997	input	993:997	input	993:997	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	11	2	theme	understanding	1742:1754	arg1	limitations					1713:1723	the current limitations	1701:1723	the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes	1701:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	11	3	theme	MS-based	1631:1638	arg1	identification					1647:1660	MS-based ligand identification	1631:1660	MS-based ligand identification	1631:1660	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	11	3	theme	MS-based	1631:1638	arg1	methods					1622:1628	two methods	1618:1628	two methods	1618:1628	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	8	4	theme	known	1234:1238	arg1	residues					1248:1255	the known binding residues	1230:1255	the known binding residues after about 100 ns simulation time	1230:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	4	5	used	used	636:639	arg2	simulations					619:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	6	6	theme	positive	849:856	arg1	control					858:864	positive control	849:864	positive control for docking	849:876	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	1	7	theme	crucial	187:193	arg1	Binding					131:137	BACKGROUND Binding	120:137	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs)	120:180	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	1	7	theme	crucial	187:193	arg1	step					195:198	a crucial step	185:198	a crucial step in leukocyte recruitment to inflamed tissues	185:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	11	8	theme	changes	1878:1884	arg1	inaccessibility					1821:1835	the numerical inaccessibility	1807:1835	the numerical inaccessibility of ligand-induced protein conformational changes	1807:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	7	9	theme	initial	1130:1136	arg1	results					1146:1152	initial docking results	1130:1152	initial docking results	1130:1152	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	2	10	theme	dp6	302:304	arg1	fraction					306:313	the HS dp6 fraction	295:313	the HS dp6 fraction	295:313	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	5	11	theme	high	771:774	arg1	binding					795:801	selective and high affinity chemokine binding	757:801	binding	795:801	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	8	12	theme	simulation	1276:1285	arg1	time					1287:1290	about 100 ns simulation time	1263:1290	about 100 ns simulation time	1263:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	0	13	from	complex	56:62	arg1	simulations					19:29	Molecular dynamics simulations	0:29	Molecular dynamics simulations of the chemokine CCL2 in complex with pull	0:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	7	14	theme	dynamics	1095:1102	arg1	simulations					1104:1114	molecular dynamics simulations	1085:1114	molecular dynamics simulations	1085:1114	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	1	15	from	step	195:198	arg1	recruitment					213:223	leukocyte recruitment	203:223	leukocyte recruitment to inflamed tissues	203:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	11	16	theme	numerical	1811:1819	arg1	inaccessibility					1821:1835	the numerical inaccessibility	1807:1835	the numerical inaccessibility of ligand-induced protein conformational changes	1807:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	9	17	theme	specific	1325:1332	arg1	ligands					1358:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	2	18	theme	disaccharide	256:267	arg1	analysis					283:290	A disaccharide compositional analysis	254:290	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction	246:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	1	19	theme	chemokines	142:151	arg1	Binding					131:137	BACKGROUND Binding	120:137	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs)	120:180	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	1	19	theme	chemokines	142:151	arg1	step					195:198	a crucial step	185:198	a crucial step in leukocyte recruitment to inflamed tissues	185:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	9	20	theme	unspecific	1338:1347	arg1	ligands					1358:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	0	21	from	simulations	19:29	arg1	complex					56:62	complex	56:62	complex with pull	56:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	11	22	theme	protein	1855:1861	arg1	changes					1878:1884	ligand-induced protein conformational changes	1840:1884	ligand-induced protein conformational changes	1840:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	9	23	theme	GAG	1354:1356	arg1	ligands					1358:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	10	24	theme	enthalpy/entropy	1491:1506	arg1	balance					1508:1514	the delicate enthalpy/entropy balance	1478:1514	the delicate enthalpy/entropy balance of GAG binding	1478:1529	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	11	25	theme	GENERAL	1574:1580	arg1	SIGNIFICANCE					1582:1593	GENERAL SIGNIFICANCE	1574:1593	GENERAL SIGNIFICANCE	1574:1593	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	0	26	theme	heparan	87:93	arg1	sulfate					95:101	heparan sulfate	87:101	heparan sulfate hexasaccharides	87:117	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	0	27	from	CCL2	48:51	arg1	complex					56:62	complex	56:62	complex with pull	56:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	6	28	used	used	985:988	arg2	structure					971:979	the previously liganded ATIII structure	941:979	the previously liganded ATIII structure	941:979	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	6	28	used	used	985:988	arg2	input					993:997	input	993:997	input	993:997	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	10	29	theme	binding	1523:1529	arg1	balance					1508:1514	the delicate enthalpy/entropy balance	1478:1514	the delicate enthalpy/entropy balance of GAG binding	1478:1529	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	8	30	with	contact	1217:1223	arg1	residues					1248:1255	the known binding residues	1230:1255	the known binding residues after about 100 ns simulation time	1230:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	0	31	theme	Molecular	0:8	arg1	simulations					19:29	Molecular dynamics simulations	0:29	Molecular dynamics simulations of the chemokine CCL2 in complex with pull	0:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	2	32	theme	structure	417:425	arg1	suggestions					427:437	target GAG ligand structure suggestions	399:437	target GAG ligand structure suggestions	399:437	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	4	33	theme	300 ns	593:598	arg1	simulations					619:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	3	34	theme	heparan	468:474	arg1	chemokine-specific					517:534	chemokine-specific	517:534	chemokine-specific	517:534	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	34	theme	heparan	468:474	arg1	unspecific					544:553	unspecific	544:553	unspecific	544:553	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	34	theme	heparan	468:474	arg1	hexasaccharides					484:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	2	35	theme	GAG	406:408	arg1	suggestions					427:437	target GAG ligand structure suggestions	399:437	target GAG ligand structure suggestions	399:437	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	6	36	theme	complex	915:921	arg1	structure					923:931	the correct complex structure	903:931	the correct complex structure	903:931	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	11	37	theme	ligand	1767:1772	arg1	binding					1774:1780	complex ligand binding	1759:1780	complex ligand binding	1759:1780	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	6	38	theme	Antithromin-III/heparin	814:836	arg1	complex					838:844	the Antithromin-III/heparin complex	810:844	the Antithromin-III/heparin complex	810:844	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	0	39	theme	CCL2	48:51	arg1	simulations					19:29	Molecular dynamics simulations	0:29	Molecular dynamics simulations of the chemokine CCL2 in complex with pull	0:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	5	40	with	Hexasaccharides	682:696	arg1	sulfations					708:717	four sulfations	703:717	four sulfations	703:717	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	5	40	with	Hexasaccharides	682:696	arg1	acetylations					726:737	no acetylations	723:737	no acetylations	723:737	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	2	41	theme	MS	335:336	arg1	analysis					338:345	MS analysis	335:345	MS analysis of the CCL2-depleted dp6 fraction	335:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	11	42	theme	molecular	1666:1674	arg1	modelling					1676:1684	molecular modelling	1666:1684	molecular modelling	1666:1684	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	11	42	theme	molecular	1666:1674	arg1	methods					1622:1628	two methods	1618:1628	two methods	1618:1628	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	3	43	theme	sulfate	476:482	arg1	chemokine-specific					517:534	chemokine-specific	517:534	chemokine-specific	517:534	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	43	theme	sulfate	476:482	arg1	unspecific					544:553	unspecific	544:553	unspecific	544:553	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	43	theme	sulfate	476:482	arg1	hexasaccharides					484:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	2	44	theme	fraction	372:379	arg1	analysis					338:345	MS analysis	335:345	MS analysis of the CCL2-depleted dp6 fraction	335:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	8	45	theme	GAG	1178:1180	arg1	oligosaccharides					1182:1197	all four GAG oligosaccharides	1169:1197	all four GAG oligosaccharides	1169:1197	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	10	46	theme	molecular	1410:1418	arg1	simulations					1429:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	2	47	theme	CCL2-depleted	354:366	arg1	fraction					372:379	the CCL2-depleted dp6 fraction	350:379	the CCL2-depleted dp6 fraction	350:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	5	48	theme	RESULTS	674:680	arg1	Hexasaccharides					682:696	RESULTS Hexasaccharides	674:696	RESULTS Hexasaccharides with four sulfations and no acetylations	674:737	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	4	49	theme	dynamics	610:617	arg1	simulations					619:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	11	50	theme	current	1705:1711	arg1	limitations					1713:1723	the current limitations	1701:1723	the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes	1701:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	2	51	theme	HS	299:300	arg1	fraction					306:313	the HS dp6 fraction	295:313	the HS dp6 fraction	295:313	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	6	52	theme	liganded	956:963	arg1	structure					971:979	the previously liganded ATIII structure	941:979	the previously liganded ATIII structure	941:979	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	6	52	theme	liganded	956:963	arg1	input					993:997	input	993:997	input	993:997	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	0	53	with	complex	56:62	arg1	pull					69:72	pull	69:72	pull	69:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	2	54	dep	METHODS	246:252	arg1	analysis					283:290	A disaccharide compositional analysis	254:290	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction	246:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	11	55	theme	molecular	1732:1740	arg1	understanding					1742:1754	our molecular understanding	1728:1754	our molecular understanding of complex ligand binding	1728:1780	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	7	56	theme	docking	1138:1144	arg1	results					1146:1152	initial docking results	1130:1152	initial docking results	1130:1152	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	11	57	theme	ligand	1640:1645	arg1	identification					1647:1660	MS-based ligand identification	1631:1660	MS-based ligand identification	1631:1660	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	11	57	theme	ligand	1640:1645	arg1	methods					1622:1628	two methods	1618:1628	two methods	1618:1628	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	3	58	link	experimentally-derived	445:466	arg1	chemokine-specific					517:534	chemokine-specific	517:534	chemokine-specific	517:534	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	58	link	experimentally-derived	445:466	arg1	unspecific					544:553	unspecific	544:553	unspecific	544:553	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	58	link	experimentally-derived	445:466	arg1	hexasaccharides					484:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	11	59	theme	methods	1622:1628	arg1	comparison					1604:1613	the comparison	1600:1613	the comparison of two methods, MS-based ligand identification and molecular modelling	1600:1684	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	2	60	theme	fraction	306:313	arg1	analysis					283:290	A disaccharide compositional analysis	254:290	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction	246:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	8	61	theme	binding	1240:1246	arg1	residues					1248:1255	the known binding residues	1230:1255	the known binding residues after about 100 ns simulation time	1230:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	1	62	theme	leukocyte	203:211	arg1	recruitment					213:223	leukocyte recruitment	203:223	leukocyte recruitment to inflamed tissues	203:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	5	63	theme	affinity	776:783	arg1	binding					795:801	selective and high affinity chemokine binding	757:801	binding	795:801	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	7	64	theme	molecular	1085:1093	arg1	simulations					1104:1114	molecular dynamics simulations	1085:1114	molecular dynamics simulations	1085:1114	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	1	65	theme	BACKGROUND	120:129	arg1	Binding					131:137	BACKGROUND Binding	120:137	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs)	120:180	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	1	65	theme	BACKGROUND	120:129	arg1	step					195:198	a crucial step	185:198	a crucial step in leukocyte recruitment to inflamed tissues	185:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	8	66	theme	100 ns	1269:1274	arg1	time					1287:1290	about 100 ns simulation time	1263:1290	about 100 ns simulation time	1263:1290	We found that all four GAG oligosaccharides ended up in close contact with the known binding residues after about 100 ns simulation time.
30586626	2	67	theme	compositional	269:281	arg1	analysis					283:290	A disaccharide compositional analysis	254:290	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction	246:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	2	68	from	analysis	283:290	arg1	combination					318:328	combination	318:328	combination with MS analysis of the CCL2-depleted dp6 fraction	318:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	5	69	theme	chemokine	785:793	arg1	binding					795:801	selective and high affinity chemokine binding	757:801	binding	795:801	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	9	70	theme	CCL2	1349:1352	arg1	ligands					1358:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	specific vs. unspecific CCL2 GAG ligands	1325:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	5	71	theme	selective	757:765	arg1	binding					795:801	selective and high affinity chemokine binding	757:801	binding	795:801	RESULTS Hexasaccharides with four sulfations and no acetylations are suggested for selective and high affinity chemokine binding.
30586626	11	72	theme	conformational	1863:1876	arg1	changes					1878:1884	ligand-induced protein conformational changes	1840:1884	ligand-induced protein conformational changes	1840:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	0	73	theme	sulfate	95:101	arg1	hexasaccharides					103:117	heparan sulfate hexasaccharides	87:117	heparan sulfate hexasaccharides	87:117	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	9	74	theme	ligands	1358:1364	arg1	possible					1373:1380	possible	1373:1380	possible	1373:1380	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	9	74	theme	ligands	1358:1364	arg1	discrimination					1307:1320	A discrimination	1305:1320	A discrimination of specific vs. unspecific CCL2 GAG ligands	1305:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	2	75	with	combination	318:328	arg1	analysis					338:345	MS analysis	335:345	MS analysis of the CCL2-depleted dp6 fraction	335:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	10	76	theme	delicate	1482:1489	arg1	balance					1508:1514	the delicate enthalpy/entropy balance	1478:1514	the delicate enthalpy/entropy balance of GAG binding	1478:1529	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	11	77	theme	ligand-induced	1840:1853	arg1	changes					1878:1884	ligand-induced protein conformational changes	1840:1884	ligand-induced protein conformational changes	1840:1884	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	7	78	theme	liganded	1010:1017	arg1	structure					1019:1027	the liganded structure	1006:1027	the liganded structure	1006:1027	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	3	79	theme	experimentally-derived	445:466	arg1	chemokine-specific					517:534	chemokine-specific	517:534	chemokine-specific	517:534	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	79	theme	experimentally-derived	445:466	arg1	unspecific					544:553	unspecific	544:553	unspecific	544:553	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	3	79	theme	experimentally-derived	445:466	arg1	hexasaccharides					484:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides	440:498	Four experimentally-derived heparan sulfate hexasaccharides, two potentially chemokine-specific and two unspecific, have been docked to CCL2.
30586626	0	80	theme	dynamics	10:17	arg1	simulations					19:29	Molecular dynamics simulations	0:29	Molecular dynamics simulations of the chemokine CCL2 in complex with pull	0:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	4	81	theme	Subsequent	582:591	arg1	simulations					619:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	10	82	theme	GAG	1519:1521	arg1	binding					1523:1529	GAG binding	1519:1529	GAG binding	1519:1529	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	11	83	dep	SIGNIFICANCE	1582:1593	arg1	comparison					1604:1613	the comparison	1600:1613	the comparison of two methods, MS-based ligand identification and molecular modelling	1600:1684	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	4	84	theme	molecular	600:608	arg1	simulations					619:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations	582:629	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	11	85	theme	binding	1774:1780	arg1	understanding					1742:1754	our molecular understanding	1728:1754	our molecular understanding of complex ligand binding	1728:1780	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	2	86	theme	ligand	410:415	arg1	suggestions					427:437	target GAG ligand structure suggestions	399:437	target GAG ligand structure suggestions	399:437	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	0	87	theme	chemokine	38:46	arg1	CCL2					48:51	the chemokine CCL2	34:51	the chemokine CCL2 in complex with pull	34:72	Molecular dynamics simulations of the chemokine CCL2 in complex with pull down-derived heparan sulfate hexasaccharides.
30586626	11	88	theme	complex	1759:1765	arg1	binding					1774:1780	complex ligand binding	1759:1780	complex ligand binding	1759:1780	GENERAL SIGNIFICANCE With the comparison of two methods, MS-based ligand identification and molecular modelling, we have shown the current limitations of our molecular understanding of complex ligand binding which is could be due to the numerical inaccessibility of ligand-induced protein conformational changes.
30586626	10	89	theme	Long-time	1400:1408	arg1	simulations					1429:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	2	90	theme	target	399:404	arg1	suggestions					427:437	target GAG ligand structure suggestions	399:437	target GAG ligand structure suggestions	399:437	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30586626	6	91	theme	correct	907:913	arg1	structure					923:931	the correct complex structure	903:931	the correct complex structure	903:931	Using the Antithromin-III/heparin complex as positive control for docking, we were able to recover the correct complex structure only if the previously liganded ATIII structure was used as input.
30586626	7	92	theme	CCL2-GAG	1048:1055	arg1	complex					1057:1063	a CCL2-GAG complex	1046:1063	a CCL2-GAG complex	1046:1063	Since the liganded structure is not known for a CCL2-GAG complex, we investigated if molecular dynamics simulations could improve initial docking results.
30586626	9	93	dep	CONCLUSIONS	1293:1303	arg1	possible					1373:1380	possible	1373:1380	possible	1373:1380	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	9	93	dep	CONCLUSIONS	1293:1303	arg1	discrimination					1307:1320	A discrimination	1305:1320	A discrimination of specific vs. unspecific CCL2 GAG ligands	1305:1364	CONCLUSIONS A discrimination of specific vs. unspecific CCL2 GAG ligands is not possible by this approach.
30586626	1	94	theme	inflamed	228:235	arg1	tissues					237:243	inflamed tissues	228:243	inflamed tissues	228:243	BACKGROUND Binding of chemokines to glycosaminoglycans (GAGs) is a crucial step in leukocyte recruitment to inflamed tissues.
30586626	4	95	theme	docked	656:661	arg1	complexes					663:671	the docked complexes	652:671	the docked complexes	652:671	Subsequent 300 ns molecular dynamics simulations were used to improve the docked complexes.
30586626	10	96	theme	dynamics	1420:1427	arg1	simulations					1429:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations	1400:1439	Long-time molecular dynamics simulations are, however, well suited to capture the delicate enthalpy/entropy balance of GAG binding and improve results obtained from docking.
30586626	2	97	theme	dp6	368:370	arg1	fraction					372:379	the CCL2-depleted dp6 fraction	350:379	the CCL2-depleted dp6 fraction	350:379	METHODS A disaccharide compositional analysis of the HS dp6 fraction in combination with MS analysis of the CCL2-depleted dp6 fraction was the basis for target GAG ligand structure suggestions.
30324318	0	0	theme	various	103:109	arg1	species					121:127	various shellfish species	103:127	various shellfish species	103:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	4	1	theme	-α-Gal-	848:854	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	1	theme	-α-Gal-	848:854	arg1	1 → were					856:863	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	1 → were	856:863	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	6	2	theme	comprehensive	1305:1317	arg1	knowledge					1319:1327	a more comprehensive knowledge	1298:1327	a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species	1298:1405	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	3	3	theme	hexuronic	688:696	arg1	acid					698:701	a hexuronic acid	686:701	a hexuronic acid linked to a hexosamine or a hexose	686:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	6	4	from	distribution	1339:1350	arg1	species					1399:1405	various shellfish species	1381:1405	various shellfish species	1381:1405	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	5	5	theme	shellfish	1204:1212	arg1	species					1214:1220	some shellfish species	1199:1220	some shellfish species	1199:1220	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	4	6	theme	→4	795:796	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	6	theme	→4	795:796	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	0	7	theme	shellfish	111:119	arg1	species					121:127	various shellfish species	103:127	various shellfish species	103:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	1	8	from	ingredients	179:189	arg1	shellfish					194:202	shellfish	194:202	shellfish	194:202	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	5	9	theme	genetic	1243:1249	arg1	relationship					1251:1262	the genetic relationship	1239:1262	the genetic relationship	1239:1262	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	3	10	with	non-GAGs	641:648	arg1	units					677:681	repeated disaccharide units	655:681	repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	655:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	5	11	theme	hierarchical	1106:1117	arg1	analysis					1130:1137	hierarchical clustering analysis	1106:1137	hierarchical clustering analysis	1106:1137	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	4	12	theme	β-GlcA-	834:840	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	12	theme	β-GlcA-	834:840	arg1	1 → were					856:863	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	1 → were	856:863	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	2	13	theme	MRM	468:470	arg1	mode					473:476	the multiple reaction monitoring (MRM) mode	434:476	the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	434:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	4	14	theme	-β-GlcA-	798:805	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	14	theme	-β-GlcA-	798:805	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	2	15	theme	reaction	447:454	arg1	mode					473:476	the multiple reaction monitoring (MRM) mode	434:476	the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	434:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	2	16	theme	PMP	575:577	arg1	derivatization					580:593	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	543:593	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	543:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	5	17	theme	clustering	1119:1128	arg1	analysis					1130:1137	hierarchical clustering analysis	1106:1137	hierarchical clustering analysis	1106:1137	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	3	18	attach	linked	703:708	arg1	hexosamine					715:724	a hexosamine	713:724	a hexosamine	713:724	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	3	18	attach	linked	703:708	arg1	hexose					731:736	a hexose	729:736	a hexose	729:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	3	18	attach	linked	703:708	arg2	acid					698:701	a hexuronic acid	686:701	a hexuronic acid linked to a hexosamine or a hexose	686:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	2	19	theme	triple	481:486	arg1	spectrometer					504:515	triple quadrupole mass spectrometer	481:515	triple quadrupole mass spectrometer	481:515	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	2	20	theme	multiple	438:445	arg1	mode					473:476	the multiple reaction monitoring (MRM) mode	434:476	the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	434:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	2	21	theme	quadrupole	488:497	arg1	spectrometer					504:515	triple quadrupole mass spectrometer	481:515	triple quadrupole mass spectrometer	481:515	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	5	22	theme	analysis	1130:1137	arg1	dendrogram					1092:1101	the dendrogram	1088:1101	the dendrogram of hierarchical clustering analysis	1088:1137	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	4	23	theme	1 → 3	842:846	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	23	theme	1 → 3	842:846	arg1	1 → were					856:863	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	1 → were	856:863	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	3	24	with	glycosaminoglycans	609:626	arg1	units					677:681	repeated disaccharide units	655:681	repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	655:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	0	25	theme	Distribution	0:11	arg1	analysis					13:20	Distribution analysis	0:20	Distribution analysis of polysaccharides	0:39	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	2	26	theme	shellfish	342:350	arg1	species					324:330	21 species	321:330	21 species of edible shellfish	321:350	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	1	27	theme	delicious	226:234	arg1	foods					251:255	delicious and nutritious foods	226:255	delicious and nutritious foods	226:255	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	27	theme	delicious	226:234	arg1	ingredients					179:189	attractive functional ingredients	157:189	attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely	157:268	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	0	28	theme	polysaccharides	25:39	arg1	analysis					13:20	Distribution analysis	0:20	Distribution analysis of polysaccharides	0:39	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	4	29	theme	-α-Man-	813:819	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	29	theme	-α-Man-	813:819	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	6	30	theme	shellfish	1389:1397	arg1	species					1399:1405	various shellfish species	1381:1405	various shellfish species	1381:1405	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	2	31	theme	edible	335:340	arg1	shellfish					342:350	edible shellfish	335:350	edible shellfish	335:350	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	4	32	theme	1 → and	821:827	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	32	theme	1 → and	821:827	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	33	theme	chondroitin	765:775	arg1	heparin					786:792	heparin	786:792	heparin	786:792	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	33	theme	chondroitin	765:775	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	33	theme	chondroitin	765:775	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	33	theme	chondroitin	765:775	arg1	1 → were					856:863	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	1 → were	856:863	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	5	34	theme	quantification	928:941	arg1	results					943:949	The quantification results	924:949	The quantification results	924:949	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	3	35	theme	acid	698:701	arg1	units					677:681	repeated disaccharide units	655:681	repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	655:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	1	36	theme	Acidic	130:135	arg1	polysaccharides					137:151	Acidic polysaccharides	130:151	Acidic polysaccharides	130:151	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	36	theme	Acidic	130:135	arg1	ingredients					179:189	attractive functional ingredients	157:189	attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely	157:268	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	2	37	theme	spectrometer	504:515	arg1	mode					473:476	the multiple reaction monitoring (MRM) mode	434:476	the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	434:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	1	38	theme	nutritious	240:249	arg1	foods					251:255	delicious and nutritious foods	226:255	delicious and nutritious foods	226:255	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	38	theme	nutritious	240:249	arg1	ingredients					179:189	attractive functional ingredients	157:189	attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely	157:268	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	2	39	theme	acid	522:525	arg1	degradation					527:537	acid degradation	522:537	acid degradation	522:537	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	6	40	theme	polysaccharides	1362:1376	arg1	distribution					1339:1350	the distribution	1335:1350	the distribution of acidic polysaccharides in various shellfish species	1335:1405	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	3	41	theme	non-GAGs	641:648	arg1	total					598:602	A total	596:602	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	596:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	5	42	from	composition	1158:1168	arg1	species					1214:1220	some shellfish species	1199:1220	some shellfish species	1199:1220	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	6	43	theme	acidic	1355:1360	arg1	polysaccharides					1362:1376	acidic polysaccharides	1355:1376	acidic polysaccharides	1355:1376	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	1	44	dep	widely	263:268	arg1	world					257:261	world	257:261	world	257:261	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	5	45	theme	shellfish	1023:1031	arg1	species					1033:1039	shellfish species	1023:1039	shellfish species	1023:1039	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	2	46	theme	monitoring	456:465	arg1	mode					473:476	the multiple reaction monitoring (MRM) mode	434:476	the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	434:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	0	47	theme	acid-hexose/hexosamine	61:82	arg1	units					94:98	uronic acid-hexose/hexosamine repeating units	54:98	uronic acid-hexose/hexosamine repeating units in various shellfish species	54:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	5	48	theme	acidic	1173:1178	arg1	polysaccharides					1180:1194	acidic polysaccharides	1173:1194	acidic polysaccharides	1173:1194	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	2	49	theme	1-phenyl-3-methyl-5-pyrazolone	543:572	arg1	derivatization					580:593	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	543:593	1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	543:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	0	50	theme	uronic	54:59	arg1	units					94:98	uronic acid-hexose/hexosamine repeating units	54:98	uronic acid-hexose/hexosamine repeating units in various shellfish species	54:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	5	51	theme	polysaccharides	1180:1194	arg1	composition					1158:1168	the composition	1154:1168	the composition of acidic polysaccharides in some shellfish species	1154:1220	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	6	52	theme	various	1381:1387	arg1	species					1399:1405	various shellfish species	1381:1405	various shellfish species	1381:1405	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	2	53	theme	mass	499:502	arg1	spectrometer					504:515	triple quadrupole mass spectrometer	481:515	triple quadrupole mass spectrometer	481:515	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	0	54	from	units	94:98	arg1	species					121:127	various shellfish species	103:127	various shellfish species	103:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	3	55	theme	disaccharide	664:675	arg1	units					677:681	repeated disaccharide units	655:681	repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	655:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	4	56	theme	1 → 2	807:811	arg1	→3					829:830	→4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were	795:863	→3	829:830	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	4	56	theme	1 → 2	807:811	arg1	sulfate					777:783	chondroitin sulfate	765:783	chondroitin sulfate	765:783	Among them, chondroitin sulfate, heparin, →4)-β-GlcA-(1 → 2)-α-Man-(1 → and →3)- β-GlcA-(1 → 3)-α-Gal-(1 → were identified unambiguously by comparing with the references.
30324318	2	57	theme	acidic	293:298	arg1	polysaccharides					300:314	acidic polysaccharides	293:314	acidic polysaccharides from 21 species of edible shellfish	293:350	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	6	58	theme	present	1275:1281	arg1	study					1283:1287	the present study	1271:1287	the present study	1271:1287	Thus, the present study provides a more comprehensive knowledge about the distribution of acidic polysaccharides in various shellfish species.
30324318	1	59	theme	attractive	157:166	arg1	polysaccharides					137:151	Acidic polysaccharides	130:151	Acidic polysaccharides	130:151	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	59	theme	attractive	157:166	arg1	foods					251:255	delicious and nutritious foods	226:255	delicious and nutritious foods	226:255	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	59	theme	attractive	157:166	arg1	ingredients					179:189	attractive functional ingredients	157:189	attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely	157:268	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	3	60	theme	glycosaminoglycans	609:626	arg1	total					598:602	A total	596:602	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	596:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	5	61	theme	polysaccharide	987:1000	arg1	contents					969:976	the contents	965:976	the contents of these polysaccharide	965:1000	The quantification results revealed that the contents of these polysaccharide varied greatly among shellfish species with a maximum over 100 mg/100 g. Furthermore, the dendrogram of hierarchical clustering analysis indicated that the composition of acidic polysaccharides in some shellfish species was related with the genetic relationship.
30324318	1	62	theme	functional	168:177	arg1	polysaccharides					137:151	Acidic polysaccharides	130:151	Acidic polysaccharides	130:151	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	62	theme	functional	168:177	arg1	foods					251:255	delicious and nutritious foods	226:255	delicious and nutritious foods	226:255	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	1	62	theme	functional	168:177	arg1	ingredients					179:189	attractive functional ingredients	157:189	attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely	157:268	Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely.
30324318	2	63	from	species	324:330	arg1	polysaccharides					300:314	acidic polysaccharides	293:314	acidic polysaccharides from 21 species of edible shellfish	293:350	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	3	64	theme	repeated	655:662	arg1	units					677:681	repeated disaccharide units	655:681	repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose	655:736	A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected.
30324318	0	65	theme	repeating	84:92	arg1	units					94:98	uronic acid-hexose/hexosamine repeating units	54:98	uronic acid-hexose/hexosamine repeating units in various shellfish species	54:127	Distribution analysis of polysaccharides comprised of uronic acid-hexose/hexosamine repeating units in various shellfish species.
30324318	2	66	theme	present	278:284	arg1	study					286:290	the present study	274:290	the present study	274:290	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
30324318	2	67	theme	repeated	405:412	arg1	disaccharides					414:426	their repeated disaccharides	399:426	their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization	399:593	In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.
31252017	3	0	theme	average	314:320	arg1	weight					331:336	Its average molecule weight	310:336	Its average molecule weight	310:336	Its average molecule weight was 99.2 kDa.
31252017	3	1	theme	molecule	322:329	arg1	weight					331:336	Its average molecule weight	310:336	Its average molecule weight	310:336	Its average molecule weight was 99.2 kDa.
31252017	8	2	theme	ICS1	903:906	arg1	levels					887:892	expression levels	876:892	expression levels of EDS1, ICS1, and PR1 involved in SA pathway	876:938	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	8	3	theme	related	822:828	arg1	proteins					830:837	pathogen related proteins	813:837	pathogen related proteins	813:837	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	1	4	theme	Dendrobium	169:178	arg1	Lindl					187:191	Dendrobium nobile Lindl	169:191	Dendrobium nobile Lindl	169:191	A neutral polysaccharide separated from Dendrobium nobile Lindl was designated as DNPE6(4).
31252017	7	5	theme	excellent	699:707	arg1	activity					720:727	excellent protective activity	699:727	excellent protective activity against TMV	699:739	Notably, DNPE6(4) exhibited excellent protective activity against TMV.
31252017	0	6	theme	tobacco	100:106	arg1	defense					108:114	tobacco defense	100:114	tobacco defense against TMV	100:126	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	8	7	theme	pathogen	813:820	arg1	proteins					830:837	pathogen related proteins	813:837	pathogen related proteins	813:837	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	7	8	theme	protective	709:718	arg1	activity					720:727	excellent protective activity	699:727	excellent protective activity against TMV	699:739	Notably, DNPE6(4) exhibited excellent protective activity against TMV.
31252017	4	9	from	Galp	399:402	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	0	10	located	found	17:21	arg1	Lindl					44:48	Dendrobium nobile Lindl	26:48	Dendrobium nobile Lindl	26:48	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	0	10	located	found	17:21	arg2	polysaccharide					2:15	A polysaccharide	0:15	A polysaccharide found in Dendrobium nobile Lindl	0:48	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	2	11	theme	spectral	278:285	arg1	analysis					300:307	spectral and chemical analysis	278:307	spectral and chemical analysis	278:307	It was structurally characterized using a combination of spectral and chemical analysis.
31252017	8	12	dep	found	870:874	arg1	up-regulated					940:951	up-regulated	940:951	found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment	870:976	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	8	13	theme	EDS1	897:900	arg1	levels					887:892	expression levels	876:892	expression levels of EDS1, ICS1, and PR1 involved in SA pathway	876:938	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	6	14	theme	first	651:655	arg1	time					657:660	the first time	647:660	the first time	647:660	The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo.
31252017	1	15	theme	nobile	180:185	arg1	Lindl					187:191	Dendrobium nobile Lindl	169:191	Dendrobium nobile Lindl	169:191	A neutral polysaccharide separated from Dendrobium nobile Lindl was designated as DNPE6(4).
31252017	5	16	theme	-D-Galp-	527:534	arg1	-D-Manp-					543:550	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	-D-Manp-	543:550	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	4	17	from	Glcp	393:396	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	8	18	theme	expression	876:885	arg1	levels					887:892	expression levels	876:892	expression levels of EDS1, ICS1, and PR1 involved in SA pathway	876:938	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	8	19	theme	DNPE6	959:963	arg1	treatment					968:976	DNPE6(4) treatment	959:976	DNPE6(4) treatment	959:976	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	5	20	theme	-L-Araf-	479:486	arg1	-D-Manp-					543:550	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	-D-Manp-	543:550	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	5	21	dep	-D-Manp-	543:550	arg1	→1					508:509	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	→1	508:509	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	4	22	from	Araf	387:390	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	11	23	theme	promising	1232:1240	arg1	agent					1252:1256	a promising antiviral agent	1230:1256	a promising antiviral agent for future study	1230:1273	This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study.
31252017	11	23	theme	promising	1232:1240	arg1	DNPE6					1217:1221	DNPE6(4)	1217:1224	DNPE6(4)	1217:1224	This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study.
31252017	5	24	theme	-D-Galp-	511:518	arg1	-D-Manp-					543:550	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	-D-Manp-	543:550	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	2	25	theme	analysis	300:307	arg1	combination					263:273	a combination	261:273	a combination of spectral and chemical analysis	261:307	It was structurally characterized using a combination of spectral and chemical analysis.
31252017	11	26	theme	antiviral	1242:1250	arg1	agent					1252:1256	a promising antiviral agent	1230:1256	a promising antiviral agent for future study	1230:1273	This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study.
31252017	11	26	theme	antiviral	1242:1250	arg1	DNPE6					1217:1221	DNPE6(4)	1217:1224	DNPE6(4)	1217:1224	This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study.
31252017	10	27	theme	tobacco	1153:1159	arg1	defense					1161:1167	the tobacco defense	1149:1167	the tobacco defense against TMV	1149:1179	All these findings revealed DNPE6(4) was an elicitor to stimulate calcium signaling pathway to enhance the tobacco defense against TMV.
31252017	10	28	theme	signaling	1120:1128	arg1	pathway					1130:1136	calcium signaling pathway	1112:1136	calcium signaling pathway	1112:1136	All these findings revealed DNPE6(4) was an elicitor to stimulate calcium signaling pathway to enhance the tobacco defense against TMV.
31252017	9	29	theme	same	1030:1033	arg1	condition					1035:1043	the same condition	1026:1043	the same condition	1026:1043	In addition, some defense enzymes increased in the same condition.
31252017	2	30	theme	chemical	291:298	arg1	analysis					300:307	spectral and chemical analysis	278:307	spectral and chemical analysis	278:307	It was structurally characterized using a combination of spectral and chemical analysis.
31252017	5	31	theme	linkage	457:463	arg1	types					465:469	Their linkage types	451:469	Their linkage types	451:469	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	0	32	theme	nobile	37:42	arg1	Lindl					44:48	Dendrobium nobile Lindl	26:48	Dendrobium nobile Lindl	26:48	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	4	33	theme	molar	419:423	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	0	34	theme	Dendrobium	26:35	arg1	Lindl					44:48	Dendrobium nobile Lindl	26:48	Dendrobium nobile Lindl	26:48	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	8	35	theme	PR1	913:915	arg1	levels					887:892	expression levels	876:892	expression levels of EDS1, ICS1, and PR1 involved in SA pathway	876:938	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	4	36	theme	2.5:0.9:0.3:0.8	434:448	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	6	37	contain	have	605:608	arg2	anti-TMV					610:617	anti-TMV	610:617	anti-TMV	610:617	The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo.
31252017	6	37	contain	have	605:608	arg1	polysaccharide					577:590	The polysaccharide	573:590	The polysaccharide	573:590	The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo.
31252017	6	37	contain	have	605:608	arg2	activities					632:641	anti-CMV activities	623:641	anti-CMV activities	623:641	The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo.
31252017	10	38	dep	elicitor	1090:1097	arg1	4					1080:1080	4	1080:1080	4	1080:1080	All these findings revealed DNPE6(4) was an elicitor to stimulate calcium signaling pathway to enhance the tobacco defense against TMV.
31252017	8	39	theme	SA	929:930	arg1	pathway					932:938	SA pathway	929:938	SA pathway	929:938	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	5	40	theme	-D-Glcp-	495:502	arg1	-D-Manp-					543:550	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	-D-Manp-	543:550	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	4	41	theme	monosaccharide	356:369	arg1	Araf					387:390	Araf	387:390	Araf	387:390	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	4	41	theme	monosaccharide	356:369	arg1	composition					371:381	The monosaccharide composition	352:381	The monosaccharide composition	352:381	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	8	42	theme	several	755:761	arg1	proteins					763:770	several proteins	755:770	several proteins related to calcium signaling pathway	755:807	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	6	43	theme	anti-CMV	623:630	arg1	activities					632:641	anti-CMV activities	623:641	anti-CMV activities	623:641	The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo.
31252017	4	44	from	Manp	409:412	arg1	ratio					425:429	a molar ratio	417:429	a molar ratio of 2.5:0.9:0.3:0.8	417:448	The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:0.3:0.8.
31252017	5	45	theme	4→	504:505	arg1	-D-Manp-					543:550	→1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3	476:552	-D-Manp-	543:550	Their linkage types were →1)-L-Araf-(3→, →1)-D-Glcp-(4→, →1)-D-Galp-(3→, →1)-D-Galp-(6→, →1)-D-Manp-(3, 6→, and T-D-Manp.
31252017	8	46	theme	related	772:778	arg1	proteins					763:770	several proteins	755:770	several proteins related to calcium signaling pathway	755:807	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	8	47	theme	signaling	791:799	arg1	pathway					801:807	calcium signaling pathway	783:807	calcium signaling pathway	783:807	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	1	48	theme	neutral	131:137	arg1	DNPE6					211:215	DNPE6	211:215	DNPE6(4)	211:218	A neutral polysaccharide separated from Dendrobium nobile Lindl was designated as DNPE6(4).
31252017	1	48	theme	neutral	131:137	arg1	polysaccharide					139:152	A neutral polysaccharide	129:152	A neutral polysaccharide separated from Dendrobium nobile Lindl	129:191	A neutral polysaccharide separated from Dendrobium nobile Lindl was designated as DNPE6(4).
31252017	9	49	theme	defense	997:1003	arg1	enzymes					1005:1011	some defense enzymes	992:1011	some defense enzymes	992:1011	In addition, some defense enzymes increased in the same condition.
31252017	10	50	theme	calcium	1112:1118	arg1	pathway					1130:1136	calcium signaling pathway	1112:1136	calcium signaling pathway	1112:1136	All these findings revealed DNPE6(4) was an elicitor to stimulate calcium signaling pathway to enhance the tobacco defense against TMV.
31252017	0	51	theme	signaling	69:77	arg1	pathway					79:85	calcium signaling pathway	61:85	calcium signaling pathway	61:85	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	8	52	theme	calcium	783:789	arg1	pathway					801:807	calcium signaling pathway	783:807	calcium signaling pathway	783:807	Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment.
31252017	0	53	theme	calcium	61:67	arg1	pathway					79:85	calcium signaling pathway	61:85	calcium signaling pathway	61:85	A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV.
31252017	11	54	theme	future	1262:1267	arg1	study					1269:1273	future study	1262:1273	future study	1262:1273	This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study.
29990554	6	0	theme	functionalized	1361:1374	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	0	theme	functionalized	1361:1374	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	5	1	theme	recovery	1114:1121	arg1	candidate					1129:1137	a novel enhanced oil recovery (EOR) candidate	1093:1137	a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume	1093:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	0	2	theme	oil	95:97	arg1	recovery					99:106	oil recovery	95:106	oil recovery	95:106	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	5	3	theme	water	1243:1247	arg1	percentage					1253:1262	the oil recovery factor and water cut percentage	1215:1262	percentage	1253:1262	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	1	4	theme	modified	190:197	arg1	biopolymers					199:209	modified biopolymers	190:209	modified biopolymers	190:209	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	5	5	theme	oil	1110:1112	arg1	candidate					1129:1137	a novel enhanced oil recovery (EOR) candidate	1093:1137	a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume	1093:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	0	6	theme	chemical	116:123	arg1	technologies					134:145	chemical flooding technologies	116:145	chemical flooding technologies	116:145	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	3	7	theme	silica	805:810	arg1	seeds					812:816	silica seeds	805:816	silica seeds	805:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	4	8	theme	thermal	1008:1014	arg1	analysis					1016:1023	thermal analysis	1008:1023	thermal analysis	1008:1023	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	2	9	theme	oil	349:351	arg1	applications					362:373	enhanced oil recovery applications	340:373	enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment	340:469	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	2	10	theme	recovery	353:360	arg1	applications					362:373	enhanced oil recovery applications	340:373	enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment	340:469	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	4	11	theme	different	893:901	arg1	techniques					917:926	different spectroscopic techniques	893:926	different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM	893:1001	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	2	12	theme	enhanced	340:347	arg1	applications					362:373	enhanced oil recovery applications	340:373	enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment	340:469	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	6	13	theme	enhanced	1439:1446	arg1	applications					1461:1472	enhanced oil recovery applications	1439:1472	enhanced oil recovery applications	1439:1472	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	14	theme	co-imidazolium/silica	1390:1410	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	14	theme	co-imidazolium/silica	1390:1410	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	3	15	theme	environmentally	603:617	arg1	oxidants					628:635	environmentally friendly oxidants	603:635	environmentally friendly oxidants	603:635	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	0	16	theme	flooding	125:132	arg1	technologies					134:145	chemical flooding technologies	116:145	chemical flooding technologies	116:145	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	5	17	theme	oil	1219:1221	arg1	factor					1232:1237	the oil recovery factor and water cut percentage	1215:1262	factor	1232:1237	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	6	18	theme	acid	1385:1388	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	18	theme	acid	1385:1388	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	1	19	from	flooding	156:163	arg1	reservoirs					173:182	HPHT reservoirs	168:182	HPHT reservoirs using modified biopolymers	168:209	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	2	20	theme	starch	325:330	arg1	limitations					303:313	limitations	303:313	limitations of native starch	303:330	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	3	21	theme	starch	519:524	arg1	biopolymer					526:535	the starch biopolymer	515:535	the starch biopolymer	515:535	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	6	22	theme	saturation	1587:1596	arg1	%					1569:1569	39%	1567:1569	39% of residual oil saturation	1567:1596	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	22	theme	saturation	1587:1596	arg1	saturation					1587:1596	residual oil saturation	1574:1596	residual oil saturation	1574:1596	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	5	23	theme	recovery	1223:1230	arg1	factor					1232:1237	the oil recovery factor and water cut percentage	1215:1262	factor	1232:1237	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	6	24	theme	sulfonic	1376:1383	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	24	theme	sulfonic	1376:1383	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	2	25	theme	bacterial	385:393	arg1	stability					426:434	thermal and ionic stability	408:434	stability	426:434	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	2	25	theme	bacterial	385:393	arg1	degradation					395:405	bacterial degradation	385:405	bacterial degradation	385:405	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	6	26	theme	oil	1583:1585	arg1	saturation					1587:1596	residual oil saturation	1574:1596	residual oil saturation	1574:1596	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	5	27	theme	injected	1287:1294	arg1	volume					1301:1306	injected pore volume	1287:1306	injected pore volume	1287:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	5	28	theme	cut	1249:1251	arg1	percentage					1253:1262	the oil recovery factor and water cut percentage	1215:1262	percentage	1253:1262	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	5	29	theme	prepared	1071:1078	arg1	composite					1080:1088	the prepared composite	1067:1088	the prepared composite	1067:1088	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	4	30	theme	spectroscopic	903:915	arg1	techniques					917:926	different spectroscopic techniques	893:926	different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM	893:1001	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	2	31	theme	reservoir	449:457	arg1	environment					459:469	severe reservoir environment	442:469	severe reservoir environment	442:469	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	0	32	theme	functionalized	20:33	arg1	acid					44:47	starch functionalized sulfonic acid	13:47	starch functionalized sulfonic acid	13:47	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	5	33	theme	pore	1296:1299	arg1	volume					1301:1306	injected pore volume	1287:1306	injected pore volume	1287:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	5	34	theme	composite	1080:1088	arg1	Evaluation					1053:1062	Evaluation	1053:1062	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.	1053:1307	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	3	35	theme	thiol	542:546	arg1	derivative					548:557	thiol derivative	542:557	thiol derivative	542:557	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	0	36	theme	starch	13:18	arg1	acid					44:47	starch functionalized sulfonic acid	13:47	starch functionalized sulfonic acid	13:47	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	2	37	theme	severe	442:447	arg1	environment					459:469	severe reservoir environment	442:469	severe reservoir environment	442:469	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	3	38	theme	free	747:750	arg1	polymerization					775:788	free radical/redox emulsion polymerization	747:788	free radical/redox emulsion polymerization in presence of silica seeds	747:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	2	39	theme	ionic	420:424	arg1	stability					426:434	thermal and ionic stability	408:434	stability	426:434	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	2	39	theme	ionic	420:424	arg1	degradation					395:405	bacterial degradation	385:405	bacterial degradation	385:405	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	6	40	theme	flooding	1313:1320	arg1	data					1322:1325	The flooding data	1309:1325	The flooding data	1309:1325	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	1	41	theme	incremental	240:250	arg1	attention					252:260	incremental attention nowadays	240:269	incremental attention nowadays	240:269	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	0	42	theme	acid	44:47	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of starch functionalized sulfonic acid	0:47	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	2	43	theme	thermal	408:414	arg1	stability					426:434	thermal and ionic stability	408:434	stability	426:434	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	2	43	theme	thermal	408:414	arg1	degradation					395:405	bacterial degradation	385:405	bacterial degradation	385:405	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	3	44	theme	radical/redox	752:764	arg1	polymerization					775:788	free radical/redox emulsion polymerization	747:788	free radical/redox emulsion polymerization in presence of silica seeds	747:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	6	45	theme	starch	1354:1359	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	45	theme	starch	1354:1359	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	3	46	theme	biopolymer	526:535	arg1	functionalization					494:510	functionalization	494:510	functionalization of the starch biopolymer with thiol derivative	494:557	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	0	47	theme	sulfonic	35:42	arg1	acid					44:47	starch functionalized sulfonic acid	13:47	starch functionalized sulfonic acid	13:47	Synthesis of starch functionalized sulfonic acid co-imidazolium/silica composite for improving oil recovery through chemical flooding technologies.
29990554	3	48	theme	starch	681:686	arg1	derivative					688:697	starch derivative	681:697	starch derivative	681:697	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	6	49	theme	high	1494:1497	arg1	temperature					1499:1509	high temperature and salinity conditions	1494:1533	temperature	1499:1509	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	5	50	theme	simulated	1178:1186	arg1	condition					1198:1206	simulated reservoir condition	1178:1206	simulated reservoir condition	1178:1206	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	6	51	theme	salinity	1515:1522	arg1	conditions					1524:1533	high temperature and salinity conditions	1494:1533	conditions	1524:1533	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	3	52	with	functionalization	494:510	arg1	derivative					548:557	thiol derivative	542:557	thiol derivative	542:557	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	5	53	theme	novel	1095:1099	arg1	candidate					1129:1137	a novel enhanced oil recovery (EOR) candidate	1093:1137	a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume	1093:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	6	54	theme	oil	1448:1450	arg1	applications					1461:1472	enhanced oil recovery applications	1439:1472	enhanced oil recovery applications	1439:1472	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	3	55	theme	present	479:485	arg1	study					487:491	the present study	475:491	the present study	475:491	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	6	56	theme	recovery	1452:1459	arg1	applications					1461:1472	enhanced oil recovery applications	1439:1472	enhanced oil recovery applications	1439:1472	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	4	57	theme	particles	959:967	arg1	size					969:972	particles size	959:972	particles size measured through DLS and TEM	959:1001	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	3	58	theme	sulfonic	586:593	arg1	acid					595:598	sulfonic acid	586:598	sulfonic acid	586:598	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	1	59	theme	Polymer	148:154	arg1	flooding					156:163	Polymer flooding	148:163	Polymer flooding in HPHT reservoirs using modified biopolymers	148:209	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	4	60	theme	TGA	1039:1041	arg1	analysis					1043:1050	TGA analysis	1039:1050	TGA analysis	1039:1050	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	2	61	theme	native	318:323	arg1	starch					325:330	native starch	318:330	native starch	318:330	Some literature reported about limitations of native starch through enhanced oil recovery applications regarding bacterial degradation, thermal and ionic stability under severe reservoir environment.
29990554	1	62	dep	attention	252:260	arg1	nowadays					262:269	nowadays	262:269	nowadays	262:269	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	6	63	theme	prepared	1345:1352	arg1	favorable					1425:1433	favorable	1425:1433	favorable	1425:1433	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	6	63	theme	prepared	1345:1352	arg1	composite					1412:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	the prepared starch functionalized sulfonic acid co-imidazolium/silica composite	1341:1420	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	4	64	theme	structure	854:862	arg1	determination					864:876	structure determination	854:876	structure determination	854:876	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	6	65	theme	residual	1574:1581	arg1	oil					1583:1585	residual oil	1574:1585	residual oil saturation	1574:1596	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	3	66	theme	friendly	619:626	arg1	oxidants					628:635	environmentally friendly oxidants	603:635	environmentally friendly oxidants	603:635	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	3	67	theme	emulsion	766:773	arg1	polymerization					775:788	free radical/redox emulsion polymerization	747:788	free radical/redox emulsion polymerization in presence of silica seeds	747:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	3	68	theme	vinyl-containing	718:733	arg1	monomers					735:742	vinyl-containing monomers	718:742	vinyl-containing monomers	718:742	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	5	69	theme	reservoir	1188:1196	arg1	condition					1198:1206	simulated reservoir condition	1178:1206	simulated reservoir condition	1178:1206	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	6	70	theme	recovery	1543:1550	arg1	factor					1552:1557	the recovery factor	1539:1557	the recovery factor	1539:1557	The flooding data indicate that the prepared starch functionalized sulfonic acid co-imidazolium/silica composite is favorable for enhanced oil recovery applications as it can withstand high temperature and salinity conditions and the recovery factor reaches 39% of residual oil saturation.
29990554	5	71	theme	enhanced	1101:1108	arg1	candidate					1129:1137	a novel enhanced oil recovery (EOR) candidate	1093:1137	a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume	1093:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	5	72	theme	EOR	1124:1126	arg1	candidate					1129:1137	a novel enhanced oil recovery (EOR) candidate	1093:1137	a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume	1093:1306	Evaluation of the prepared composite as a novel enhanced oil recovery (EOR) candidate as well as the precursors conducted at simulated reservoir condition, where the oil recovery factor and water cut percentage calculated relevant to injected pore volume.
29990554	1	73	from	composites	221:230	arg1	reservoirs					173:182	HPHT reservoirs	168:182	HPHT reservoirs using modified biopolymers	168:209	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	1	74	theme	HPHT	168:171	arg1	reservoirs					173:182	HPHT reservoirs	168:182	HPHT reservoirs using modified biopolymers	168:209	Polymer flooding in HPHT reservoirs using modified biopolymers and their composites acquire incremental attention nowadays.
29990554	3	75	from	polymerization	775:788	arg1	presence					793:800	presence	793:800	presence of silica seeds	793:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	3	76	theme	seeds	812:816	arg1	presence					793:800	presence	793:800	presence of silica seeds	793:816	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	4	77	theme	1H	945:946	arg1	FTIR					939:942	FTIR	939:942	FTIR	939:942	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	4	77	theme	1H	945:946	arg1	NMR					948:950	1H NMR	945:950	1H NMR	945:950	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29990554	3	78	theme	thiol	577:581	arg1	oxidation					564:572	oxidation	564:572	oxidation of thiol to sulfonic acid by environmentally friendly oxidants	564:635	In the present study, functionalization of the starch biopolymer with thiol derivative then oxidation of thiol to sulfonic acid by environmentally friendly oxidants has been prepared and confirmed, after that starch derivative copolymerized with vinyl-containing monomers by free radical/redox emulsion polymerization in presence of silica seeds.
29990554	4	79	theme	Spectroscopic	819:831	arg1	characterization					833:848	Spectroscopic characterization	819:848	Spectroscopic characterization	819:848	Spectroscopic characterization and structure determination carried out by different spectroscopic techniques comprising FTIR, 1H NMR, while particles size measured through DLS and TEM, and thermal analysis determined by TGA analysis.
29806458	3	0	theme	intact	676:681	arg1	walls					688:692	intact cell walls	676:692	intact cell walls	676:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	10	1	theme	growth	1729:1734	arg1	times					1736:1740	different growth times	1719:1740	different growth times	1719:1740	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	5	2	theme	cell	1162:1165	arg1	samples					1167:1173	intact bacterial cell samples	1145:1173	intact bacterial cell samples	1145:1173	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	4	3	theme	cell	849:852	arg1	walls					854:858	isolated cell walls	840:858	isolated cell walls	840:858	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	11	4	theme	activity	1885:1892	arg1	context					1863:1869	the context	1859:1869	the context of whole-cell activity	1859:1892	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	3	5	theme	purified	695:702	arg1	PG					704:705	purified PG	695:705	purified PG	695:705	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	10	6	located	detected	1663:1670	arg1	spectra					1686:1692	whole-cell spectra	1675:1692	whole-cell spectra of bacteria harvested at different growth times	1675:1740	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	10	6	located	detected	1663:1670	arg2	Changes					1607:1613	Changes	1607:1613	Changes consistent with altered PG and WTA content	1607:1656	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	1	7	theme	macromolecular	199:212	arg1	wall					219:222	a multilayered macromolecular cell wall	184:222	a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics	184:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	7	theme	macromolecular	199:212	arg1	essential					232:240	essential	232:240	essential	232:240	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	7	theme	macromolecular	199:212	arg1	target					281:286	a major target	273:286	a major target for antibiotics	273:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	6	8	theme	NMR	1210:1212	arg1	contributions					1214:1226	The distinguishing whole-cell 13C NMR contributions	1176:1226	The distinguishing whole-cell 13C NMR contributions associated with PG	1176:1245	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	3	9	theme	purified	712:719	arg1	WTA					721:723	purified WTA	712:723	purified WTA	712:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	5	10	theme	WTA	1031:1033	arg1	signatures					1044:1053	WTA spectral signatures	1031:1053	WTA spectral signatures	1031:1053	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	2	11	theme	cell	309:312	arg1	wall					314:317	The cell wall	305:317	The cell wall of Staphylococcus aureus	305:342	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	8	12	from	nitrogens	1427:1435	arg1	PG					1440:1441	PG	1440:1441	PG	1440:1441	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	8	12	from	nitrogens	1427:1435	arg1	WTA					1490:1492	WTA	1490:1492	WTA	1490:1492	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	4	13	theme	teichoic	901:908	arg1	content					915:921	estimating teichoic acid content	890:921	estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls	890:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	6	14	theme	13C	1206:1208	arg1	contributions					1214:1226	The distinguishing whole-cell 13C NMR contributions	1176:1226	The distinguishing whole-cell 13C NMR contributions associated with PG	1176:1245	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	11	15	theme	composition	1844:1854	arg1	parameters					1830:1839	quantitative parameters	1817:1839	quantitative parameters of composition	1817:1854	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	3	16	theme	polarization	586:597	arg1	angle					605:609	13C cross polarization magic angle	576:609	13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA	576:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	8	17	theme	esterified	1451:1460	arg1	nitrogens					1477:1485	the esterified d-alanyl amine nitrogens	1447:1485	the esterified d-alanyl amine nitrogens in WTA	1447:1492	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	6	18	theme	distinguishing	1180:1193	arg1	contributions					1214:1226	The distinguishing whole-cell 13C NMR contributions	1176:1226	The distinguishing whole-cell 13C NMR contributions associated with PG	1176:1245	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	3	19	theme	13C	576:578	arg1	angle					605:609	13C cross polarization magic angle	576:609	13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA	576:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	9	20	theme	13C	1495:1497	arg1	analysis					1503:1510	13C NMR analysis	1495:1510	13C NMR analysis	1495:1510	13C NMR analysis was performed with samples at natural abundance and included 10 whole-cell sample comparisons.
29806458	4	21	theme	attractive	864:873	arg1	alternative					875:885	an attractive alternative	861:885	an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls	861:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	8	22	theme	amine	1471:1475	arg1	nitrogens					1477:1485	the esterified d-alanyl amine nitrogens	1447:1485	the esterified d-alanyl amine nitrogens in WTA	1447:1492	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	6	23	theme	sugar	1273:1277	arg1	carbons					1279:1285	the GlcNAc-MurNAc sugar carbons	1255:1285	the GlcNAc-MurNAc sugar carbons	1255:1285	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	3	24	theme	solid-state	620:630	arg1	spectra					665:671	solid-state nuclear magnetic resonance (NMR) spectra	620:671	solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls	620:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	2	25	theme	insoluble	479:487	arg1	matrix					489:494	a heterogeneous and insoluble matrix	459:494	a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis	459:556	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	9	26	theme	NMR	1499:1501	arg1	analysis					1503:1510	13C NMR analysis	1495:1510	13C NMR analysis	1495:1510	13C NMR analysis was performed with samples at natural abundance and included 10 whole-cell sample comparisons.
29806458	5	27	theme	NMR	1089:1091	arg1	spectra					1093:1099	whole-cell NMR spectra	1078:1099	whole-cell NMR spectra	1078:1099	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	4	28	from	differences	765:775	arg1	polymers					788:795	the two polymers	780:795	the two polymers	780:795	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	5	29	used	used	1105:1108	arg2	PG					1024:1025	unique PG	1017:1025	unique PG	1017:1025	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	9	30	theme	natural	1542:1548	arg1	abundance					1550:1558	natural abundance	1542:1558	natural abundance	1542:1558	13C NMR analysis was performed with samples at natural abundance and included 10 whole-cell sample comparisons.
29806458	0	31	from	Alterations	43:53	arg1	aureus					73:78	Staphylococcus aureus	58:78	Staphylococcus aureus	58:78	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	4	32	theme	clear	749:753	arg1	differences					765:775	the clear molecular differences	745:775	the clear molecular differences in the two polymers	745:795	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	0	33	theme	Nuclear	108:114	arg1	Resonance					125:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	0	34	from	Levels	32:37	arg1	aureus					73:78	Staphylococcus aureus	58:78	Staphylococcus aureus	58:78	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	0	35	theme	Peptidoglycan	0:12	arg1	Levels					32:37	Peptidoglycan and Teichoic Acid Levels	0:37	Peptidoglycan and Teichoic Acid Levels	0:37	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	5	36	theme	WTA	1128:1130	arg1	levels					1132:1137	PG and WTA levels	1121:1137	PG and WTA levels among intact bacterial cell samples	1121:1173	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	7	37	from	backbone	1357:1364	arg1	carbons					1325:1331	carbons	1325:1331	carbons from the phosphoribitol backbone	1325:1364	WTA contributes carbons from the phosphoribitol backbone.
29806458	8	38	theme	15N	1382:1384	arg1	signatures					1395:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	2	39	theme	teichoic	420:427	arg1	WTA					435:437	WTA	435:437	WTA	435:437	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	39	theme	teichoic	420:427	arg1	acid					429:432	wall teichoic acid	415:432	wall teichoic acid (WTA)	415:438	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	39	theme	teichoic	420:427	arg1	components					380:389	two major structural components	359:389	two major structural components	359:389	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	0	40	theme	Teichoic	18:25	arg1	Acid					27:30	Teichoic Acid	18:30	Teichoic Acid	18:30	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	9	41	theme	sample	1587:1592	arg1	comparisons					1594:1604	10 whole-cell sample comparisons	1573:1604	10 whole-cell sample comparisons	1573:1604	13C NMR analysis was performed with samples at natural abundance and included 10 whole-cell sample comparisons.
29806458	4	42	theme	cell	973:976	arg1	walls					978:982	completely pyrolyzed cell walls	952:982	completely pyrolyzed cell walls	952:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	10	43	theme	whole-cell	1675:1684	arg1	spectra					1686:1692	whole-cell spectra	1675:1692	whole-cell spectra of bacteria harvested at different growth times	1675:1740	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	3	44	theme	magnetic	640:647	arg1	resonance					649:657	nuclear magnetic resonance	632:657	solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls	620:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	3	44	theme	magnetic	640:647	arg1	NMR					660:662	NMR	660:662	NMR	660:662	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	8	45	theme	glycyl	1414:1419	arg1	nitrogens					1427:1435	glycyl amide nitrogens	1414:1435	glycyl amide nitrogens in PG	1414:1441	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	10	46	theme	different	1719:1727	arg1	times					1736:1740	different growth times	1719:1740	different growth times	1719:1740	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	5	47	theme	PG	1121:1122	arg1	levels					1132:1137	PG and WTA levels	1121:1137	PG and WTA levels among intact bacterial cell samples	1121:1173	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	0	48	dep	Levels	32:37	arg1	Resonance					125:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	10	49	with	consistent	1615:1624	arg1	PG					1639:1640	altered PG	1631:1640	altered PG	1631:1640	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	10	49	with	consistent	1615:1624	arg1	content					1650:1656	WTA content	1646:1656	WTA content	1646:1656	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	1	50	theme	cell	245:248	arg1	survival					250:257	cell survival	245:257	cell survival	245:257	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	4	51	from	analysis	940:947	arg1	content					915:921	estimating teichoic acid content	890:921	estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls	890:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	6	52	theme	GlcNAc-MurNAc	1259:1271	arg1	carbons					1279:1285	the GlcNAc-MurNAc sugar carbons	1255:1285	the GlcNAc-MurNAc sugar carbons	1255:1285	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	2	53	theme	major	363:367	arg1	acid					429:432	wall teichoic acid	415:432	wall teichoic acid (WTA)	415:438	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	53	theme	major	363:367	arg1	peptidoglycan					392:404	peptidoglycan	392:404	peptidoglycan (PG)	392:409	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	53	theme	major	363:367	arg1	components					380:389	two major structural components	359:389	two major structural components	359:389	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	5	54	theme	bacterial	1152:1160	arg1	samples					1167:1173	intact bacterial cell samples	1145:1173	intact bacterial cell samples	1145:1173	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	4	55	theme	WTA	833:835	arg1	quantification					808:821	quantification	808:821	quantification of PG and WTA	808:835	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	3	56	theme	cell	683:686	arg1	walls					688:692	intact cell walls	676:692	intact cell walls	676:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	1	57	theme	major	275:279	arg1	wall					219:222	a multilayered macromolecular cell wall	184:222	a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics	184:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	57	theme	major	275:279	arg1	target					281:286	a major target	273:286	a major target for antibiotics	273:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	4	58	theme	isolated	840:847	arg1	walls					854:858	isolated cell walls	840:858	isolated cell walls	840:858	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	1	59	theme	multilayered	186:197	arg1	wall					219:222	a multilayered macromolecular cell wall	184:222	a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics	184:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	59	theme	multilayered	186:197	arg1	essential					232:240	essential	232:240	essential	232:240	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	59	theme	multilayered	186:197	arg1	target					281:286	a major target	273:286	a major target for antibiotics	273:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	10	60	theme	consistent	1615:1624	arg1	Changes					1607:1613	Changes	1607:1613	Changes consistent with altered PG and WTA content	1607:1656	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	1	61	theme	cell	214:217	arg1	wall					219:222	a multilayered macromolecular cell wall	184:222	a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics	184:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	61	theme	cell	214:217	arg1	essential					232:240	essential	232:240	essential	232:240	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	1	61	theme	cell	214:217	arg1	target					281:286	a major target	273:286	a major target for antibiotics	273:302	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	4	62	theme	PG	826:827	arg1	quantification					808:821	quantification	808:821	quantification of PG and WTA	808:835	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	11	63	theme	quantitative	1817:1828	arg1	parameters					1830:1839	quantitative parameters	1817:1839	quantitative parameters of composition	1817:1854	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	2	64	theme	compositional	535:547	arg1	analysis					549:556	quantitative compositional analysis	522:556	quantitative compositional analysis	522:556	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	11	65	theme	NMR	1804:1806	arg1	use					1786:1788	This use	1781:1788	This use of whole-cell NMR	1781:1806	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	5	66	theme	spectral	1035:1042	arg1	signatures					1044:1053	WTA spectral signatures	1031:1053	WTA spectral signatures	1031:1053	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	3	67	theme	cross	580:584	arg1	angle					605:609	13C cross polarization magic angle	576:609	13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA	576:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	4	68	theme	estimating	890:899	arg1	content					915:921	estimating teichoic acid content	890:921	estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls	890:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	8	69	theme	d-alanyl	1462:1469	arg1	nitrogens					1477:1485	the esterified d-alanyl amine nitrogens	1447:1485	the esterified d-alanyl amine nitrogens in WTA	1447:1492	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	6	70	theme	whole-cell	1195:1204	arg1	contributions					1214:1226	The distinguishing whole-cell 13C NMR contributions	1176:1226	The distinguishing whole-cell 13C NMR contributions associated with PG	1176:1245	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	10	71	theme	altered	1631:1637	arg1	PG					1639:1640	altered PG	1631:1640	altered PG	1631:1640	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	6	72	theme	glycyl	1291:1296	arg1	α-carbons					1298:1306	glycyl α-carbons	1291:1306	glycyl α-carbons	1291:1306	The distinguishing whole-cell 13C NMR contributions associated with PG include the GlcNAc-MurNAc sugar carbons and glycyl α-carbons.
29806458	5	73	theme	unique	1017:1022	arg1	PG					1024:1025	unique PG	1017:1025	unique PG	1017:1025	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	1	74	theme	Gram-positive	136:148	arg1	bacteria					150:157	Gram-positive bacteria	136:157	Gram-positive bacteria	136:157	Gram-positive bacteria surround themselves with a multilayered macromolecular cell wall that is essential to cell survival and serves as a major target for antibiotics.
29806458	7	75	theme	phosphoribitol	1342:1355	arg1	backbone					1357:1364	the phosphoribitol backbone	1338:1364	the phosphoribitol backbone	1338:1364	WTA contributes carbons from the phosphoribitol backbone.
29806458	3	76	theme	magic	599:603	arg1	angle					605:609	13C cross polarization magic angle	576:609	13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA	576:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	4	77	theme	phosphate	930:938	arg1	analysis					940:947	a phosphate analysis	928:947	a phosphate analysis of completely pyrolyzed cell walls	928:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	0	78	theme	Cell-Wall	83:91	arg1	Resonance					125:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	11	79	theme	whole-cell	1793:1802	arg1	NMR					1804:1806	whole-cell NMR	1793:1806	whole-cell NMR	1793:1806	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	2	80	theme	heterogeneous	461:473	arg1	matrix					489:494	a heterogeneous and insoluble matrix	459:494	a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis	459:556	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	0	81	theme	Whole-Cell	97:106	arg1	Resonance					125:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	8	82	theme	amide	1421:1425	arg1	nitrogens					1427:1435	glycyl amide nitrogens	1414:1435	glycyl amide nitrogens in PG	1414:1441	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	4	83	theme	acid	910:913	arg1	content					915:921	estimating teichoic acid content	890:921	estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls	890:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	10	84	theme	bacteria	1697:1704	arg1	spectra					1686:1692	whole-cell spectra	1675:1692	whole-cell spectra of bacteria harvested at different growth times	1675:1740	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	8	85	from	nitrogens	1477:1485	arg1	PG					1440:1441	PG	1440:1441	PG	1440:1441	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	8	85	from	nitrogens	1477:1485	arg1	WTA					1490:1492	WTA	1490:1492	WTA	1490:1492	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	0	86	theme	Magnetic	116:123	arg1	Resonance					125:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Cell-Wall and Whole-Cell Nuclear Magnetic Resonance	83:133	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	5	87	theme	whole-cell	1078:1087	arg1	spectra					1093:1099	whole-cell NMR spectra	1078:1099	whole-cell NMR spectra	1078:1099	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	2	88	theme	quantitative	522:533	arg1	analysis					549:556	quantitative compositional analysis	522:556	quantitative compositional analysis	522:556	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	10	89	theme	WTA	1646:1648	arg1	content					1650:1656	WTA content	1646:1656	WTA content	1646:1656	Changes consistent with altered PG and WTA content were detected in whole-cell spectra of bacteria harvested at different growth times and in cells treated with tunicamycin.
29806458	0	90	theme	Acid	27:30	arg1	Levels					32:37	Peptidoglycan and Teichoic Acid Levels	0:37	Peptidoglycan and Teichoic Acid Levels	0:37	Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.
29806458	9	91	theme	whole-cell	1576:1585	arg1	comparisons					1594:1604	10 whole-cell sample comparisons	1573:1604	10 whole-cell sample comparisons	1573:1604	13C NMR analysis was performed with samples at natural abundance and included 10 whole-cell sample comparisons.
29806458	3	92	theme	resonance	649:657	arg1	spectra					665:671	solid-state nuclear magnetic resonance (NMR) spectra	620:671	solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls	620:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	8	93	theme	Distinguishing	1367:1380	arg1	signatures					1395:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	2	94	theme	wall	415:418	arg1	WTA					435:437	WTA	435:437	WTA	435:437	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	94	theme	wall	415:418	arg1	acid					429:432	wall teichoic acid	415:432	wall teichoic acid (WTA)	415:438	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	94	theme	wall	415:418	arg1	components					380:389	two major structural components	359:389	two major structural components	359:389	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	5	95	theme	intact	1145:1150	arg1	samples					1167:1173	intact bacterial cell samples	1145:1173	intact bacterial cell samples	1145:1173	Furthermore, we discovered that unique PG and WTA spectral signatures could be identified in whole-cell NMR spectra and used to compare PG and WTA levels among intact bacterial cell samples.
29806458	4	96	theme	pyrolyzed	963:971	arg1	walls					978:982	completely pyrolyzed cell walls	952:982	completely pyrolyzed cell walls	952:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	3	97	theme	nuclear	632:638	arg1	resonance					649:657	nuclear magnetic resonance	632:657	solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls	620:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	3	97	theme	nuclear	632:638	arg1	NMR					660:662	NMR	660:662	NMR	660:662	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	8	98	theme	spectral	1386:1393	arg1	signatures					1395:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures	1367:1404	Distinguishing 15N spectral signatures include glycyl amide nitrogens in PG and the esterified d-alanyl amine nitrogens in WTA.
29806458	4	99	theme	walls	978:982	arg1	analysis					940:947	a phosphate analysis	928:947	a phosphate analysis of completely pyrolyzed cell walls	928:982	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	11	100	theme	whole-cell	1874:1883	arg1	activity					1885:1892	whole-cell activity	1874:1892	whole-cell activity	1874:1892	This use of whole-cell NMR provides quantitative parameters of composition in the context of whole-cell activity.
29806458	4	101	theme	molecular	755:763	arg1	differences					765:775	the clear molecular differences	745:775	the clear molecular differences in the two polymers	745:795	The spectra reveal the clear molecular differences in the two polymers and enable quantification of PG and WTA in isolated cell walls, an attractive alternative to estimating teichoic acid content from a phosphate analysis of completely pyrolyzed cell walls.
29806458	2	102	theme	aureus	337:342	arg1	wall					314:317	The cell wall	305:317	The cell wall of Staphylococcus aureus	305:342	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	3	103	theme	walls	688:692	arg1	PG					704:705	purified PG	695:705	purified PG	695:705	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	3	103	theme	walls	688:692	arg1	spectra					665:671	solid-state nuclear magnetic resonance (NMR) spectra	620:671	solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls	620:692	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	3	103	theme	walls	688:692	arg1	WTA					721:723	purified WTA	712:723	purified WTA	712:723	Here, we present 13C cross polarization magic angle spinning solid-state nuclear magnetic resonance (NMR) spectra of intact cell walls, purified PG, and purified WTA.
29806458	2	104	theme	structural	369:378	arg1	acid					429:432	wall teichoic acid	415:432	wall teichoic acid (WTA)	415:438	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	104	theme	structural	369:378	arg1	peptidoglycan					392:404	peptidoglycan	392:404	peptidoglycan (PG)	392:409	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
29806458	2	104	theme	structural	369:378	arg1	components					380:389	two major structural components	359:389	two major structural components	359:389	The cell wall of Staphylococcus aureus is composed of two major structural components, peptidoglycan (PG) and wall teichoic acid (WTA), together creating a heterogeneous and insoluble matrix that poses a challenge to quantitative compositional analysis.
30738901	0	0	theme	tissue	129:134	arg1	engineering					136:146	bone tissue engineering	124:146	bone tissue engineering	124:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	2	1	theme	hydrophilic	550:560	arg1	nature					562:567	hydrophilic nature	550:567	hydrophilic nature	550:567	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	1	2	theme	increasing	202:211	arg1	attention					213:221	increasing attention	202:221	increasing attention	202:221	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	0	3	theme	bone	124:127	arg1	engineering					136:146	bone tissue engineering	124:146	bone tissue engineering	124:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	8	4	theme	potential	1623:1631	arg1	biomaterial					1633:1643	a potential biomaterial	1621:1643	a potential biomaterial for BTE applications	1621:1664	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	8	4	theme	potential	1623:1631	arg1	scaffolds					1604:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	6	5	theme	composite	1362:1370	arg1	scaffolds					1372:1380	the composite scaffolds	1358:1380	the composite scaffolds	1358:1380	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	5	6	theme	fluorescence	1153:1164	arg1	emission					1166:1173	fluorescence emission	1153:1173	fluorescence emission	1153:1173	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	5	6	theme	fluorescence	1153:1164	arg1	porosity					1102:1109	The porosity	1098:1109	The porosity	1098:1109	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	3	7	theme	mechanical	919:928	arg1	properties					930:939	mechanical properties	919:939	mechanical properties	919:939	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	5	8	theme	compressive	1131:1141	arg1	strength					1143:1150	compressive strength	1131:1150	compressive strength	1131:1150	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	5	8	theme	compressive	1131:1141	arg1	porosity					1102:1109	The porosity	1098:1109	The porosity	1098:1109	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	7	9	theme	n-HA	1431:1434	arg1	Incorporation					1414:1426	Incorporation	1414:1426	Incorporation of n-HA and adsorption of FD into the composite scaffolds	1414:1484	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	0	10	link	genipin-crosslinked	15:33	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	3	11	theme	nano-scaled	607:617	arg1	n-HA					635:638	n-HA	635:638	n-HA	635:638	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	3	11	theme	nano-scaled	607:617	arg1	hydroxyapatite					619:632	nano-scaled hydroxyapatite	607:632	nano-scaled hydroxyapatite (n-HA)	607:639	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	4	12	theme	GP-crosslinked	1070:1083	arg1	matrix					1090:1095	the GP-crosslinked HPCS matrix	1066:1095	the GP-crosslinked HPCS matrix	1066:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	4	13	theme	rough	1027:1031	arg1	morphology					1033:1042	a rough morphology	1025:1042	a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix	1025:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	7	14	theme	osteoblast	1516:1525	arg1	cells					1527:1531	7F2 osteoblast cells	1512:1531	7F2 osteoblast cells	1512:1531	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	2	15	theme	tissue	467:472	arg1	engineering					474:484	bone tissue engineering	462:484	bone tissue engineering	462:484	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	2	16	theme	low	573:575	arg1	crystallinity					577:589	low crystallinity	573:589	low crystallinity	573:589	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	5	17	theme	degradation	1179:1189	arg1	rate					1191:1194	degradation rate	1179:1194	degradation rate	1179:1194	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	5	17	theme	degradation	1179:1189	arg1	porosity					1102:1109	The porosity	1098:1109	The porosity	1098:1109	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	4	18	link	GP-crosslinked	1070:1083	arg1	matrix					1090:1095	the GP-crosslinked HPCS matrix	1066:1095	the GP-crosslinked HPCS matrix	1066:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	4	19	with	structure	985:993	arg1	pores					1015:1019	interconnected pores	1000:1019	interconnected pores	1000:1019	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	4	19	with	structure	985:993	arg1	n-HA					1049:1052	n-HA	1049:1052	n-HA inserted in the GP-crosslinked HPCS matrix	1049:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	2	20	theme	bone	462:465	arg1	engineering					474:484	bone tissue engineering	462:484	bone tissue engineering	462:484	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	8	21	theme	BTE	1649:1651	arg1	applications					1653:1664	BTE applications	1649:1664	BTE applications	1649:1664	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	2	22	theme	cell	510:513	arg1	adhesion					515:522	cell adhesion	510:522	cell adhesion	510:522	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	5	23	theme	n-HA	1246:1249	arg1	contents					1251:1258	n-HA contents	1246:1258	n-HA contents	1246:1258	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	4	24	theme	HPCS	1085:1088	arg1	matrix					1090:1095	the GP-crosslinked HPCS matrix	1066:1095	the GP-crosslinked HPCS matrix	1066:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	7	25	theme	7F2	1512:1514	arg1	cells					1527:1531	7F2 osteoblast cells	1512:1531	7F2 osteoblast cells	1512:1531	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	1	26	theme	antibacterial	347:359	arg1	activities					361:370	better antioxidant and antibacterial activities	324:370	activities	361:370	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	6	27	theme	algae	1302:1306	arg1	polysaccharide					1308:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	6	27	theme	algae	1302:1306	arg1	fucoidan					1324:1331	fucoidan	1324:1331	fucoidan	1324:1331	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	1	28	theme	Hydroxypropyl	149:161	arg1	HPCS					173:176	HPCS	173:176	HPCS	173:176	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	28	theme	Hydroxypropyl	149:161	arg1	chitosan					163:170	Hydroxypropyl chitosan	149:170	Hydroxypropyl chitosan (HPCS)	149:177	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	0	29	theme	genipin-crosslinked	15:33	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	3	30	theme	HPCS	812:815	arg1	crosslinking					796:807	crosslinking	796:807	crosslinking of HPCS	796:815	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	4	31	theme	open	980:983	arg1	structure					985:993	an open structure	977:993	an open structure with interconnected pores	977:1019	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	3	32	theme	nanocomposite	701:713	arg1	scaffolds					715:723	n-HA/HPCS nanocomposite scaffolds	691:723	n-HA/HPCS nanocomposite scaffolds	691:723	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	0	33	theme	nano-hydroxyapatite/hydroxypropyl	57:89	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	4	34	theme	interconnected	1000:1013	arg1	pores					1015:1019	interconnected pores	1000:1019	interconnected pores	1000:1019	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	1	35	dep	applications	237:248	arg1	has					261:263	has	261:263	has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan	261:393	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	36	theme	enhanced	265:272	arg1	solubility					280:289	enhanced water solubility	265:289	enhanced water solubility	265:289	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	0	37	theme	fucoidan-adsorbed	39:55	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	3	38	dep	accompanied	779:789	arg1	HPCS					767:770	HPCS	767:770	HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties	767:939	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	3	38	dep	accompanied	779:789	arg1	matrix					772:777	matrix	772:777	matrix	772:777	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	7	39	from	activity	1500:1507	arg1	cells					1527:1531	7F2 osteoblast cells	1512:1531	7F2 osteoblast cells	1512:1531	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	7	40	theme	composite	1466:1474	arg1	scaffolds					1476:1484	the composite scaffolds	1462:1484	the composite scaffolds	1462:1484	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	1	41	theme	water	274:278	arg1	solubility					280:289	enhanced water solubility	265:289	enhanced water solubility	265:289	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	0	42	theme	composite	100:108	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	5	43	theme	GP	1224:1225	arg1	concentrations					1227:1240	GP concentrations	1224:1240	GP concentrations	1224:1240	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	3	44	theme	n-HA/HPCS	691:699	arg1	scaffolds					715:723	n-HA/HPCS nanocomposite scaffolds	691:723	n-HA/HPCS nanocomposite scaffolds	691:723	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	2	45	from	requirement	447:457	arg1	engineering					474:484	bone tissue engineering	462:484	bone tissue engineering	462:484	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	0	46	theme	chitosan	91:98	arg1	scaffolds					110:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds	15:118	genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering	15:146	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	3	47	theme	occurring	832:840	arg1	compound					842:849	a naturally occurring compound	820:849	a naturally occurring compound	820:849	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	3	47	theme	occurring	832:840	arg1	genipin					852:858	genipin	852:858	genipin (GP)	852:863	In this study, nano-scaled hydroxyapatite (n-HA) and HPCS were synthesized, respectively, and then n-HA/HPCS nanocomposite scaffolds were developed by incorporating n-HA into HPCS matrix accompanied with crosslinking of HPCS by a naturally occurring compound, genipin (GP), which in turn greatly altered the hydrophilicity and mechanical properties.
30738901	4	48	theme	nanocomposite	946:958	arg1	scaffolds					960:968	The nanocomposite scaffolds	942:968	The nanocomposite scaffolds	942:968	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	8	49	theme	n-HA/HPCS	1584:1592	arg1	biomaterial					1633:1643	a potential biomaterial	1621:1643	a potential biomaterial for BTE applications	1621:1664	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	8	49	theme	n-HA/HPCS	1584:1592	arg1	scaffolds					1604:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	5	50	theme	swelling	1112:1119	arg1	capacity					1121:1128	swelling capacity	1112:1128	swelling capacity	1112:1128	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	5	50	theme	swelling	1112:1119	arg1	porosity					1102:1109	The porosity	1098:1109	The porosity	1098:1109	The porosity, swelling capacity, compressive strength, fluorescence emission and degradation rate can be regulated by varying GP concentrations and n-HA contents.
30738901	6	51	theme	electrostatic	1386:1398	arg1	interactions					1400:1411	electrostatic interactions	1386:1411	electrostatic interactions	1386:1411	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	1	52	dep	antioxidant	331:341	arg1	better					324:329	better	324:329	better	324:329	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	6	53	theme	marine	1295:1300	arg1	polysaccharide					1308:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	6	53	theme	marine	1295:1300	arg1	fucoidan					1324:1331	fucoidan	1324:1331	fucoidan	1324:1331	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	4	54	with	morphology	1033:1042	arg1	pores					1015:1019	interconnected pores	1000:1019	interconnected pores	1000:1019	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	4	54	with	morphology	1033:1042	arg1	n-HA					1049:1052	n-HA	1049:1052	n-HA inserted in the GP-crosslinked HPCS matrix	1049:1095	The nanocomposite scaffolds showed an open structure with interconnected pores and a rough morphology with n-HA inserted in the GP-crosslinked HPCS matrix.
30738901	7	55	theme	ALP	1496:1498	arg1	activity					1500:1507	ALP activity	1496:1507	ALP activity in 7F2 osteoblast cells	1496:1531	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	1	56	contain	has	261:263	arg2	antioxidant					331:341	antioxidant	331:341	antioxidant	331:341	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	56	contain	has	261:263	arg2	solubility					280:289	enhanced water solubility	265:289	enhanced water solubility	265:289	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	56	contain	has	261:263	arg2	biocompatibility					302:317	excellent biocompatibility	292:317	excellent biocompatibility	292:317	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	56	contain	has	261:263	arg1	it					258:259	it	258:259	it	258:259	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	6	57	theme	osteogenic	1284:1293	arg1	polysaccharide					1308:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	6	57	theme	osteogenic	1284:1293	arg1	fucoidan					1324:1331	fucoidan	1324:1331	fucoidan	1324:1331	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	8	58	theme	FD-adsorbed	1572:1582	arg1	biomaterial					1633:1643	a potential biomaterial	1621:1643	a potential biomaterial for BTE applications	1621:1664	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	8	58	theme	FD-adsorbed	1572:1582	arg1	scaffolds					1604:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	8	59	theme	composite	1594:1602	arg1	biomaterial					1633:1643	a potential biomaterial	1621:1643	a potential biomaterial for BTE applications	1621:1664	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	8	59	theme	composite	1594:1602	arg1	scaffolds					1604:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds	1568:1612	The FD-adsorbed n-HA/HPCS composite scaffolds can be a potential biomaterial for BTE applications.
30738901	7	60	theme	adsorption	1440:1449	arg1	Incorporation					1414:1426	Incorporation	1414:1426	Incorporation of n-HA and adsorption of FD into the composite scaffolds	1414:1484	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	2	61	theme	strength	438:445	arg1	requirement					447:457	the mechanical strength requirement	423:457	the mechanical strength requirement in bone tissue engineering	423:484	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
30738901	1	62	theme	biomedical	226:235	arg1	applications					237:248	biomedical applications	226:248	biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan	226:393	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	1	63	theme	excellent	292:300	arg1	biocompatibility					302:317	excellent biocompatibility	292:317	excellent biocompatibility	292:317	Hydroxypropyl chitosan (HPCS) has recently attracted increasing attention in biomedical applications because it has enhanced water solubility, excellent biocompatibility, and better antioxidant and antibacterial activities compared with chitosan.
30738901	0	64	theme	scaffolds	110:118	arg1	Development					0:10	Development	0:10	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.	0:147	Development of genipin-crosslinked and fucoidan-adsorbed nano-hydroxyapatite/hydroxypropyl chitosan composite scaffolds for bone tissue engineering.
30738901	7	65	theme	FD	1454:1455	arg1	adsorption					1440:1449	adsorption	1440:1449	adsorption	1440:1449	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	7	65	theme	FD	1454:1455	arg1	n-HA					1431:1434	n-HA	1431:1434	n-HA	1431:1434	Incorporation of n-HA and adsorption of FD into the composite scaffolds increased ALP activity in 7F2 osteoblast cells and promoted their mineralization.
30738901	6	66	theme	osteoconductive	1264:1278	arg1	polysaccharide					1308:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide	1261:1321	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	6	66	theme	osteoconductive	1264:1278	arg1	fucoidan					1324:1331	fucoidan	1324:1331	fucoidan	1324:1331	An osteoconductive and osteogenic marine algae polysaccharide, fucoidan, was further adsorbed to the composite scaffolds via electrostatic interactions.
30738901	2	67	theme	mechanical	427:436	arg1	requirement					447:457	the mechanical strength requirement	423:457	the mechanical strength requirement in bone tissue engineering	423:484	However, HPCS doesn't meet the mechanical strength requirement in bone tissue engineering and is not suitable for cell adhesion and growth because of its hydrophilic nature and low crystallinity.
31276719	0	0	theme	citrus	101:106	arg1	peels					121:125	Korean citrus, Cheongkyool peels	94:125	peels	121:125	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	2	1	theme	cell	472:475	arg1	lines					477:481	RAW 264.7 cell lines	462:481	RAW 264.7 cell lines	462:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	7	2	theme	rhamnogalacturonan-I	1090:1109	arg1	CCE-I					1075:1079	CCE-I	1075:1079	CCE-I	1075:1079	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	7	2	theme	rhamnogalacturonan-I	1090:1109	arg1	polysaccharide					1133:1146	highly branched polysaccharide	1117:1146	highly branched polysaccharide with short arabinan and galactan side chains	1117:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	7	2	theme	rhamnogalacturonan-I	1090:1109	arg1	type					1111:1114	a rhamnogalacturonan-I type	1088:1114	a rhamnogalacturonan-I type	1088:1114	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	2	3	from	production	425:434	arg1	lines					477:481	RAW 264.7 cell lines	462:481	RAW 264.7 cell lines	462:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	0	4	theme	Korean	94:99	arg1	peels					121:125	Korean citrus, Cheongkyool peels	94:125	peels	121:125	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	1	5	theme	Korean	187:192	arg1	citrus					194:199	the Korean citrus	183:199	the Korean citrus	183:199	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	6	6	theme	sugar	961:965	arg1	composition					967:977	sugar composition	961:977	sugar composition	961:977	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	0	7	theme	Cheongkyool	109:119	arg1	peels					121:125	Korean citrus, Cheongkyool peels	94:125	peels	121:125	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	3	8	theme	IL-6	520:523	arg1	expression					506:515	the mRNA expression	497:515	the mRNA expression of IL-6, TNF-α, and iNOS	497:540	It augmented the mRNA expression of IL-6, TNF-α, and iNOS in a dose-dependent manner.
31276719	1	9	theme	pectinase	268:276	arg1	digests					278:284	the pectinase digests	264:284	the pectinase digests	264:284	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	7	10	theme	galactan	1172:1179	arg1	chains					1186:1191	short arabinan and galactan side chains	1153:1191	short arabinan and galactan side chains	1153:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	4	11	theme	p65	703:705	arg1	translocation					686:698	the nuclear translocation	674:698	the nuclear translocation of p65	674:705	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	6	12	theme	primary	864:870	arg1	structure					872:880	the primary structure	860:880	the primary structure of CCE-I that exhibited potent immunostimulatory activity	860:938	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	7	13	theme	side	1181:1184	arg1	chains					1186:1191	short arabinan and galactan side chains	1153:1191	short arabinan and galactan side chains	1153:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	7	14	theme	branched	1124:1131	arg1	polysaccharide					1133:1146	highly branched polysaccharide	1117:1146	highly branched polysaccharide with short arabinan and galactan side chains	1117:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	7	14	theme	branched	1124:1131	arg1	type					1111:1114	a rhamnogalacturonan-I type	1088:1114	a rhamnogalacturonan-I type	1088:1114	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	2	15	theme	TNF-α	445:449	arg1	production					425:434	the production	421:434	the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines	421:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	4	16	theme	MAPKs	630:634	arg1	phosphorylation					611:625	phosphorylation	611:625	phosphorylation of MAPKs and NF-κB related proteins	611:661	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	7	17	theme	arabinan	1159:1166	arg1	chains					1186:1191	short arabinan and galactan side chains	1153:1191	short arabinan and galactan side chains	1153:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	1	18	theme	gel	383:385	arg1	filtration					387:396	gel filtration	383:396	gel filtration	383:396	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	5	19	with	treatment	772:780	arg1	antibodies					796:805	specific antibodies	787:805	specific antibodies for TLR2, TLR4, and scavenger receptors	787:845	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	4	20	theme	related	646:652	arg1	proteins					654:661	NF-κB related proteins	640:661	NF-κB related proteins	640:661	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	0	21	theme	Signaling	0:8	arg1	pathway					10:16	Signaling pathway	0:16	Signaling pathway	0:16	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	3	22	theme	iNOS	537:540	arg1	expression					506:515	the mRNA expression	497:515	the mRNA expression of IL-6, TNF-α, and iNOS	497:540	It augmented the mRNA expression of IL-6, TNF-α, and iNOS in a dose-dependent manner.
31276719	4	23	theme	NF-κB	640:644	arg1	proteins					654:661	NF-κB related proteins	640:661	NF-κB related proteins	640:661	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	0	24	theme	structural	22:31	arg1	features					33:40	structural features	22:40	structural features	22:40	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	1	25	theme	Cheongkyool	289:299	arg1	peels					301:305	Cheongkyool peels	289:305	Cheongkyool peels	289:305	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	6	26	theme	immunostimulatory	913:929	arg1	activity					931:938	potent immunostimulatory activity	906:938	potent immunostimulatory activity	906:938	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	3	27	theme	TNF-α	526:530	arg1	expression					506:515	the mRNA expression	497:515	the mRNA expression of IL-6, TNF-α, and iNOS	497:540	It augmented the mRNA expression of IL-6, TNF-α, and iNOS in a dose-dependent manner.
31276719	5	28	theme	specific	787:794	arg1	antibodies					796:805	specific antibodies	787:805	specific antibodies for TLR2, TLR4, and scavenger receptors	787:845	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	2	29	theme	IL-6	439:442	arg1	production					425:434	the production	421:434	the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines	421:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	1	30	theme	peels	301:305	arg1	digests					278:284	the pectinase digests	264:284	the pectinase digests	264:284	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	2	31	theme	NO	456:457	arg1	production					425:434	the production	421:434	the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines	421:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	1	32	theme	crude	217:221	arg1	Cheongkyool					202:212	Cheongkyool	202:212	Cheongkyool	202:212	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	1	32	theme	crude	217:221	arg1	CCE-0					239:243	CCE-0	239:243	CCE-0	239:243	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	1	32	theme	crude	217:221	arg1	polysaccharide					223:236	a crude polysaccharide	215:236	a crude polysaccharide (CCE-0)	215:244	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	2	33	theme	RAW	462:464	arg1	lines					477:481	RAW 264.7 cell lines	462:481	RAW 264.7 cell lines	462:481	CCE-I highly enhanced the production of IL-6, TNF-α, and NO in RAW 264.7 cell lines.
31276719	6	34	theme	potent	906:911	arg1	activity					931:938	potent immunostimulatory activity	906:938	potent immunostimulatory activity	906:938	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	5	35	from	effect	712:717	arg1	production					743:752	IL-6 production	738:752	IL-6 production	738:752	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	5	35	from	effect	712:717	arg1	NO					731:732	NO	731:732	NO	731:732	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	3	36	theme	dose-dependent	547:560	arg1	manner					562:567	a dose-dependent manner	545:567	a dose-dependent manner	545:567	It augmented the mRNA expression of IL-6, TNF-α, and iNOS in a dose-dependent manner.
31276719	6	37	theme	oligosaccharide	1002:1016	arg1	analysis					1018:1025	oligosaccharide analysis	1002:1025	oligosaccharide analysis	1002:1025	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	5	38	theme	IL-6	738:741	arg1	production					743:752	IL-6 production	738:752	IL-6 production	738:752	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	0	39	theme	pectic	67:72	arg1	polysaccharide					74:87	macrophage-activating pectic polysaccharide	45:87	macrophage-activating pectic polysaccharide	45:87	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	5	40	theme	CCE-I	722:726	arg1	effect					712:717	The effect	708:717	The effect of CCE-I on NO and IL-6 production	708:752	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	1	41	attach	isolated	250:257	arg2	polysaccharide					223:236	a crude polysaccharide	215:236	a crude polysaccharide (CCE-0)	215:244	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	1	41	attach	isolated	250:257	arg1	digests					278:284	the pectinase digests	264:284	the pectinase digests	264:284	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	1	41	attach	isolated	250:257	arg2	Cheongkyool					202:212	Cheongkyool	202:212	Cheongkyool	202:212	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	4	42	theme	nuclear	678:684	arg1	translocation					686:698	the nuclear translocation	674:698	the nuclear translocation of p65	674:705	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	0	43	theme	macrophage-activating	45:65	arg1	polysaccharide					74:87	macrophage-activating pectic polysaccharide	45:87	macrophage-activating pectic polysaccharide	45:87	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	6	44	theme	CCE-I	885:889	arg1	structure					872:880	the primary structure	860:880	the primary structure of CCE-I that exhibited potent immunostimulatory activity	860:938	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	1	45	theme	complex	323:329	arg1	CCE-I					346:350	the complex polysaccharide CCE-I	319:350	the complex polysaccharide CCE-I	319:350	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	0	46	from	peels	121:125	arg1	pathway					10:16	Signaling pathway	0:16	Signaling pathway	0:16	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	0	46	from	peels	121:125	arg1	features					33:40	structural features	22:40	structural features	22:40	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	4	47	theme	proteins	654:661	arg1	phosphorylation					611:625	phosphorylation	611:625	phosphorylation of MAPKs and NF-κB related proteins	611:661	Moreover, CCE-I dose-dependently induced phosphorylation of MAPKs and NF-κB related proteins and led to the nuclear translocation of p65.
31276719	7	48	with	polysaccharide	1133:1146	arg1	chains					1186:1191	short arabinan and galactan side chains	1153:1191	short arabinan and galactan side chains	1153:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	1	49	theme	immuno-stimulating	148:165	arg1	ingredient					167:176	the immuno-stimulating ingredient	144:176	the immuno-stimulating ingredient from the Korean citrus	144:199	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	5	50	theme	scavenger	827:835	arg1	receptors					837:845	scavenger receptors	827:845	scavenger receptors	827:845	The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors.
31276719	6	51	theme	linkage	980:986	arg1	analysis					988:995	linkage analysis	980:995	linkage analysis	980:995	Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after β-elimination.
31276719	0	52	theme	polysaccharide	74:87	arg1	pathway					10:16	Signaling pathway	0:16	Signaling pathway	0:16	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	0	52	theme	polysaccharide	74:87	arg1	features					33:40	structural features	22:40	structural features	22:40	Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels.
31276719	1	53	theme	polysaccharide	331:344	arg1	CCE-I					346:350	the complex polysaccharide CCE-I	319:350	the complex polysaccharide CCE-I	319:350	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	7	54	theme	short	1153:1157	arg1	chains					1186:1191	short arabinan and galactan side chains	1153:1191	short arabinan and galactan side chains	1153:1191	The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains.
31276719	1	55	from	citrus	194:199	arg1	ingredient					167:176	the immuno-stimulating ingredient	144:176	the immuno-stimulating ingredient from the Korean citrus	144:199	To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration.
31276719	3	56	theme	mRNA	501:504	arg1	expression					506:515	the mRNA expression	497:515	the mRNA expression of IL-6, TNF-α, and iNOS	497:540	It augmented the mRNA expression of IL-6, TNF-α, and iNOS in a dose-dependent manner.
29710405	0	0	theme	Prebiotic	102:110	arg1	Potential					112:120	Prebiotic Potential	102:120	Prebiotic Potential	102:120	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	3	1	theme	till	663:666	arg1	48 h					668:671	till 48 h	663:671	till 48 h	663:671	Fermentation digesta samples are taken for analysis in time up till 48 h.
29710405	4	2	theme	IMMP-27	802:808	arg1	fermentation					810:821	48 h. IMMP-27 fermentation	796:821	48 h. IMMP-27 fermentation	796:821	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	4	3	link	linked	875:880	arg1	residues					891:898	α-(1→4) linked glucosyl residues	867:898	α-(1→4) linked glucosyl residues	867:898	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	1	4	theme	fatty	282:286	arg1	acids					288:292	short chain fatty acids	270:292	short chain fatty acids (SCFAs)	270:300	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	4	theme	fatty	282:286	arg1	SCFAs					295:299	SCFAs	295:299	SCFAs	295:299	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	6	5	theme	extracellular	1259:1271	arg1	enzymes					1273:1279	extracellular enzymes	1259:1279	extracellular enzymes	1259:1279	The polysaccharide fraction is degraded into isomalto-oligosaccharides (IMOs) mainly by extracellular enzymes.
29710405	8	6	theme	Lactobacillus	1426:1438	arg1	diversity					1366:1374	Overall microbial diversity	1348:1374	Overall microbial diversity	1348:1374	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	8	6	theme	Lactobacillus	1426:1438	arg1	abundance					1393:1401	the relative abundance	1380:1401	the relative abundance of Bifidobacterium and Lactobacillus	1380:1438	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	4	7	theme	48 h.	796:800	arg1	fermentation					810:821	48 h. IMMP-27 fermentation	796:821	48 h. IMMP-27 fermentation	796:821	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	8	8	theme	microbial	1356:1364	arg1	diversity					1366:1374	Overall microbial diversity	1348:1374	Overall microbial diversity	1348:1374	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	5	9	theme	propionic	1138:1146	arg1	acid					1148:1151	propionic acid	1138:1151	propionic acid	1138:1151	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	1	10	theme	acids	288:292	arg1	production					256:265	production	256:265	production of short chain fatty acids (SCFAs)	256:300	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	10	theme	acids	288:292	arg1	acid					310:313	lactic acid	303:313	lactic acid	303:313	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	10	theme	acids	288:292	arg1	IMMPs					249:253	IMMPs	249:253	IMMPs	249:253	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	10	theme	acids	288:292	arg1	acid					329:332	succinic acid	320:332	succinic acid	320:332	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	4	11	theme	glucosyl	882:889	arg1	residues					891:898	α-(1→4) linked glucosyl residues	867:898	α-(1→4) linked glucosyl residues	867:898	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	1	12	theme	enzyme	345:350	arg1	activity					352:359	enzyme activity	345:359	enzyme activity	345:359	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	4	13	link	linked	937:942	arg1	glucoses					944:951	α-(1→6) linked glucoses	929:951	α-(1→6) linked glucoses	929:951	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	9	14	theme	α-	1537:1538	arg1	units					1560:1564	α-(1→6) linked glucose units	1537:1564	α-(1→6) linked glucose units	1537:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	4	15	theme	glycosidic	702:711	arg1	linkages					713:720	α-(1→6) glycosidic linkages	694:720	α-(1→6) glycosidic linkages	694:720	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	9	16	link	linked	1545:1550	arg1	units					1560:1564	α-(1→6) linked glucose units	1537:1564	α-(1→6) linked glucose units	1537:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	5	17	theme	succinic	1091:1098	arg1	acid					1100:1103	succinic acid	1091:1103	succinic acid	1091:1103	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	4	18	theme	linked	875:880	arg1	residues					891:898	α-(1→4) linked glucosyl residues	867:898	α-(1→4) linked glucosyl residues	867:898	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	9	19	theme	fermentable	1577:1587	arg1	segments					1525:1532	CONCLUSION IMMP containing segments	1498:1532	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units	1498:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	9	19	theme	fermentable	1577:1587	arg1	fibers					1589:1594	slowly fermentable fibers	1570:1594	slowly fermentable fibers with prebiotic potential	1570:1619	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	4	20	theme	glucose	1017:1023	arg1	moieties					1025:1032	α-(1→4) linked glucose moieties	1002:1032	α-(1→4) linked glucose moieties	1002:1032	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	4	21	theme	linked	1010:1015	arg1	moieties					1025:1032	α-(1→4) linked glucose moieties	1002:1032	α-(1→4) linked glucose moieties	1002:1032	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	1	22	theme	microbiota	365:374	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	2	23	theme	fecal	584:588	arg1	inoculum					590:597	human fecal inoculum	578:597	human fecal inoculum	578:597	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	4	24	theme	linkages	713:720	arg1	fermentation					678:689	The fermentation	674:689	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation	674:821	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	4	25	theme	linked	937:942	arg1	glucoses					944:951	α-(1→6) linked glucoses	929:951	α-(1→6) linked glucoses	929:951	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	5	26	theme	acetic	1075:1080	arg1	acid					1082:1085	acetic acid	1075:1085	acetic acid	1075:1085	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	2	27	theme	human	578:582	arg1	inoculum					590:597	human fecal inoculum	578:597	human fecal inoculum	578:597	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	4	28	theme	moieties	1025:1032	arg1	depletion					989:997	the depletion	985:997	the depletion of α-(1→4) linked glucose moieties	985:1032	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	0	29	theme	In	0:1	arg1	Behavior					22:29	In Vitro Fermentation Behavior	0:29	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum	0:90	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	5	30	theme	high	1057:1060	arg1	amounts					1062:1068	high amounts	1057:1068	high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid	1057:1168	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	9	31	theme	prebiotic	1601:1609	arg1	potential					1611:1619	prebiotic potential	1601:1619	prebiotic potential	1601:1619	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	2	32	theme	digestible	511:520	arg1	starch					522:527	digestible starch	511:527	digestible starch segments	511:536	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	5	33	with	amounts	1062:1068	arg1	acid					1082:1085	acetic acid	1075:1085	acetic acid	1075:1085	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	5	33	with	amounts	1062:1068	arg1	acid					1100:1103	succinic acid	1091:1103	succinic acid	1091:1103	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	7	34	theme	smaller	1286:1292	arg1	IMOs					1294:1297	The smaller IMOs	1282:1297	The smaller IMOs	1282:1297	The smaller IMOs are further degraded by cell-associated enzymes.
29710405	4	35	theme	α-	1002:1003	arg1	moieties					1025:1032	α-(1→4) linked glucose moieties	1002:1032	α-(1→4) linked glucose moieties	1002:1032	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	0	36	theme	Fermentation	9:20	arg1	Behavior					22:29	In Vitro Fermentation Behavior	0:29	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum	0:90	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	1	37	theme	lactic	303:308	arg1	acid					310:313	lactic acid	303:313	lactic acid	303:313	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	3	38	theme	digesta	613:619	arg1	samples					621:627	Fermentation digesta samples	600:627	Fermentation digesta samples	600:627	Fermentation digesta samples are taken for analysis in time up till 48 h.
29710405	9	39	theme	containing	1514:1523	arg1	segments					1525:1532	CONCLUSION IMMP containing segments	1498:1532	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units	1498:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	9	39	theme	containing	1514:1523	arg1	fibers					1589:1594	slowly fermentable fibers	1570:1594	slowly fermentable fibers with prebiotic potential	1570:1619	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	8	40	theme	IMMPs	1491:1495	arg1	fermentation					1475:1486	the fermentation	1471:1486	the fermentation of IMMPs	1471:1495	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	0	41	theme	Isomalto/Malto-Polysaccharides	34:63	arg1	Behavior					22:29	In Vitro Fermentation Behavior	0:29	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum	0:90	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	1	42	theme	acid	310:313	arg1	activity					352:359	enzyme activity	345:359	enzyme activity	345:359	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	42	theme	acid	310:313	arg1	degradation					234:244	degradation	234:244	degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition	234:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	42	theme	acid	310:313	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	5	43	theme	major	1115:1119	arg1	products					1121:1128	the major products	1111:1128	the major products next to propionic acid and butyric acid	1111:1168	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	9	44	dep	units	1560:1564	arg1	1→6					1540:1542	1→6	1540:1542	1→6	1540:1542	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	1	45	theme	IMMPs	249:253	arg1	activity					352:359	enzyme activity	345:359	enzyme activity	345:359	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	45	theme	IMMPs	249:253	arg1	degradation					234:244	degradation	234:244	degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition	234:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	45	theme	IMMPs	249:253	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	2	46	dep	IMMP-94	409:415	arg1	%					420:420	94%	418:420	94% α-(1→6) glycosidic linkages	418:448	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	0	47	theme	Human	71:75	arg1	Inoculum					83:90	Human Fecal Inoculum	71:90	Human Fecal Inoculum	71:90	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	3	48	theme	Fermentation	600:611	arg1	samples					621:627	Fermentation digesta samples	600:627	Fermentation digesta samples	600:627	Fermentation digesta samples are taken for analysis in time up till 48 h.
29710405	2	49	dep	%	420:420	arg1	linkages					441:448	α-(1→6) glycosidic linkages	422:448	94% α-(1→6) glycosidic linkages	418:448	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	6	50	theme	polysaccharide	1175:1188	arg1	fraction					1190:1197	The polysaccharide fraction	1171:1197	The polysaccharide fraction	1171:1197	The polysaccharide fraction is degraded into isomalto-oligosaccharides (IMOs) mainly by extracellular enzymes.
29710405	2	51	theme	glycosidic	430:439	arg1	linkages					441:448	α-(1→6) glycosidic linkages	422:448	94% α-(1→6) glycosidic linkages	418:448	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	1	52	theme	succinic	320:327	arg1	acid					329:332	succinic acid	320:332	succinic acid	320:332	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	2	53	dep	IMMP-dig27	474:483	arg1	IMMP-27					486:492	IMMP-27	486:492	IMMP-27	486:492	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	9	54	theme	units	1560:1564	arg1	segments					1525:1532	CONCLUSION IMMP containing segments	1498:1532	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units	1498:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	9	54	theme	units	1560:1564	arg1	fibers					1589:1594	slowly fermentable fibers	1570:1594	slowly fermentable fibers with prebiotic potential	1570:1619	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	4	55	from	fermentation	678:689	arg1	starts					758:763	IMMP-94, IMMP-96, and IMMP-dig27 starts	725:763	IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation	725:821	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	1	56	theme	acid	329:332	arg1	activity					352:359	enzyme activity	345:359	enzyme activity	345:359	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	56	theme	acid	329:332	arg1	degradation					234:244	degradation	234:244	degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition	234:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	56	theme	acid	329:332	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	2	57	theme	segments	529:536	arg1	removal					500:506	removal	500:506	removal of digestible starch segments	500:536	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	4	58	theme	glucoses	944:951	arg1	utilization					914:924	the utilization	910:924	the utilization of α-(1→6) linked glucoses	910:951	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	2	59	dep	METHODS	389:395	arg1	IMMP-27					461:467	IMMP-27	461:467	IMMP-27	461:467	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	2	59	dep	METHODS	389:395	arg1	IMMP-dig27					474:483	IMMP-dig27	474:483	IMMP-dig27	474:483	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	2	59	dep	METHODS	389:395	arg1	IMMP-96					452:458	IMMP-96	452:458	IMMP-96	452:458	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	2	59	dep	METHODS	389:395	arg1	METHODS					389:395	METHODS	389:395	METHODS	389:395	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	2	59	dep	METHODS	389:395	arg1	IMMP-94					409:415	IMMP-94	409:415	IMMP-94	409:415	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	4	60	link	linked	1010:1015	arg1	moieties					1025:1032	α-(1→4) linked glucose moieties	1002:1032	α-(1→4) linked glucose moieties	1002:1032	The fermentation of α-(1→6) glycosidic linkages in IMMP-94, IMMP-96, and IMMP-dig27 starts after 12 h and finishes within 48 h. IMMP-27 fermentation starts directly after inoculation utilizing α-(1→4) linked glucosyl residues; however, the utilization of α-(1→6) linked glucoses is delayed and start only after the depletion of α-(1→4) linked glucose moieties.
29710405	1	61	theme	intestinal	153:162	arg1	fermentation					164:175	intestinal fermentation	153:175	intestinal fermentation of isomalto/malto-polysaccharides (IMMPs)	153:217	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	5	62	theme	butyric	1157:1163	arg1	acid					1165:1168	butyric acid	1157:1168	butyric acid	1157:1168	SCFAs are produced in high amounts with acetic acid and succinic acid being the major products next to propionic acid and butyric acid.
29710405	1	63	theme	production	256:265	arg1	activity					352:359	enzyme activity	345:359	enzyme activity	345:359	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	63	theme	production	256:265	arg1	degradation					234:244	degradation	234:244	degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition	234:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	63	theme	production	256:265	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	2	64	theme	starch	522:527	arg1	segments					529:536	digestible starch segments	511:536	digestible starch segments	511:536	METHODS AND RESULTS IMMP-94 (94% α-(1→6) glycosidic linkages), IMMP-96, IMMP-27, and IMMP-dig27 (IMMP-27 after removal of digestible starch segments) are fermented batchwise in vitro using human fecal inoculum.
29710405	8	65	theme	Overall	1348:1354	arg1	diversity					1366:1374	Overall microbial diversity	1348:1374	Overall microbial diversity	1348:1374	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	7	66	theme	cell-associated	1323:1337	arg1	enzymes					1339:1345	cell-associated enzymes	1323:1345	cell-associated enzymes	1323:1345	The smaller IMOs are further degraded by cell-associated enzymes.
29710405	9	67	theme	glucose	1552:1558	arg1	units					1560:1564	α-(1→6) linked glucose units	1537:1564	α-(1→6) linked glucose units	1537:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	9	68	with	fibers	1589:1594	arg1	potential					1611:1619	prebiotic potential	1601:1619	prebiotic potential	1601:1619	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	8	69	theme	relative	1384:1391	arg1	abundance					1393:1401	the relative abundance	1380:1401	the relative abundance of Bifidobacterium and Lactobacillus	1380:1438	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	9	70	theme	linked	1545:1550	arg1	units					1560:1564	α-(1→6) linked glucose units	1537:1564	α-(1→6) linked glucose units	1537:1564	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	1	71	dep	SCOPE	123:127	arg1	characterize					140:151	characterize	140:151	characterize	140:151	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	9	72	dep	containing	1514:1523	arg1	CONCLUSION					1498:1507	CONCLUSION	1498:1507	CONCLUSION	1498:1507	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	9	72	dep	containing	1514:1523	arg1	IMMP					1509:1512	IMMP	1509:1512	IMMP	1509:1512	CONCLUSION IMMP containing segments of α-(1→6) linked glucose units are slowly fermentable fibers with prebiotic potential.
29710405	1	73	theme	short	270:274	arg1	acids					288:292	short chain fatty acids	270:292	short chain fatty acids (SCFAs)	270:300	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	73	theme	short	270:274	arg1	SCFAs					295:299	SCFAs	295:299	SCFAs	295:299	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	0	74	theme	Fecal	77:81	arg1	Inoculum					83:90	Human Fecal Inoculum	71:90	Human Fecal Inoculum	71:90	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	8	75	theme	Bifidobacterium	1406:1420	arg1	diversity					1366:1374	Overall microbial diversity	1348:1374	Overall microbial diversity	1348:1374	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	8	75	theme	Bifidobacterium	1406:1420	arg1	abundance					1393:1401	the relative abundance	1380:1401	the relative abundance of Bifidobacterium and Lactobacillus	1380:1438	Overall microbial diversity and the relative abundance of Bifidobacterium and Lactobacillus, significantly increase during the fermentation of IMMPs.
29710405	0	76	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.
29710405	1	77	theme	isomalto/malto-polysaccharides	180:209	arg1	fermentation					164:175	intestinal fermentation	153:175	intestinal fermentation of isomalto/malto-polysaccharides (IMMPs)	153:217	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	78	theme	chain	276:280	arg1	acids					288:292	short chain fatty acids	270:292	short chain fatty acids (SCFAs)	270:300	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
29710405	1	78	theme	chain	276:280	arg1	SCFAs					295:299	SCFAs	295:299	SCFAs	295:299	SCOPE This study characterize intestinal fermentation of isomalto/malto-polysaccharides (IMMPs), by monitoring degradation of IMMPs, production of short chain fatty acids (SCFAs), lactic acid, and succinic acid as well as enzyme activity and microbiota composition.
31862374	3	0	theme	Candida	1038:1044	arg1	NCCLS					1056:1060	NCCLS 11	1056:1063	NCCLS 11	1056:1063	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	3	0	theme	Candida	1038:1044	arg1	albicans					1046:1053	Candida albicans	1038:1053	Candida albicans (NCCLS 11)	1038:1064	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	1	1	theme	nano-composites	273:287	arg1	method					147:152	novel synthesizing method	128:152	novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	128:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	2	2	from	shape	624:628	arg1	nano-scale					600:609	nano-scale	600:609	nano-scale	600:609	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	3	3	theme	Gram-negative	814:826	arg1	bacteria					828:835	(i) Gram-negative bacteria	810:835	(i) Gram-negative bacteria	810:835	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	1	4	theme	acids	219:223	arg1	nano-composites					273:287	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	7	5	from	nanocomposites	1445:1458	arg1	close					1431:1435	close	1431:1435	close	1431:1435	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	7	5	from	nanocomposites	1445:1458	arg1	values					1419:1424	The MIC values	1411:1424	The MIC values	1411:1424	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	4	6	theme	rapid	1138:1142	arg1	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	7	7	theme	zone	1476:1479	arg1	measurements					1481:1492	high clear zone measurements	1465:1492	high clear zone measurements in the same concentration in the case of Trp-TOC	1465:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	7	8	theme	high	1465:1468	arg1	measurements					1481:1492	high clear zone measurements	1465:1492	high clear zone measurements in the same concentration in the case of Trp-TOC	1465:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	2	9	theme	electron	480:487	arg1	SEM					500:502	SEM	500:502	SEM	500:502	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	9	theme	electron	480:487	arg1	microscope					489:498	scanning electron microscope	471:498	scanning electron microscope(SEM)	471:503	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	10	theme	potential	292:300	arg1	agents					330:335	potential antimicrobial biocompatible agents	292:335	potential antimicrobial biocompatible agents	292:335	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	2	11	dep	analysis	446:453	arg1	DTGA					464:467	DTGA	464:467	DTGA	464:467	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	11	dep	analysis	446:453	arg1	TGA					456:458	TGA	456:458	TGA	456:458	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	12	theme	composites	586:595	arg1	synthesis					573:581	the synthesis	569:581	the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively	569:761	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	13	theme	l-phenyl	226:233	arg1	Phe					244:246	Phe	244:246	Phe	244:246	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	13	theme	l-phenyl	226:233	arg1	acids					219:223	oxidized cellulose (extracted from bagasse) (TOC) amino acids	163:223	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	13	theme	l-phenyl	226:233	arg1	alanine					235:241	l-phenyl alanine	226:241	l-phenyl alanine (Phe)	226:247	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	0	14	theme	amino	87:91	arg1	acid					93:96	amino acid	87:96	amino acid	87:96	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	1	15	theme	antimicrobial	302:314	arg1	agents					330:335	potential antimicrobial biocompatible agents	292:335	potential antimicrobial biocompatible agents	292:335	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	2	16	from	composites	586:595	arg1	nano-scale					600:609	nano-scale	600:609	nano-scale	600:609	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	17	with	shape	624:628	arg1	size					652:655	average particle size 72	635:658	average particle size 72	635:658	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	17	with	shape	624:628	arg1	44.37 nm					664:671	44.37 nm	664:671	44.37 nm	664:671	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	18	theme	biocompatible	316:328	arg1	agents					330:335	potential antimicrobial biocompatible agents	292:335	potential antimicrobial biocompatible agents	292:335	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	3	19	theme	Gram-positive	913:925	arg1	bacteria					927:934	Gram-positive bacteria	913:934	Gram-positive bacteria	913:934	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	2	20	theme	produced	342:349	arg1	nanocomposites					351:364	The produced nanocomposites	338:364	The produced nanocomposites	338:364	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	21	theme	TEMPO	157:161	arg1	nano-composites					273:287	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	3	22	theme	antimicrobial	768:780	arg1	studies					782:788	The antimicrobial studies	764:788	The antimicrobial studies	764:788	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	0	23	theme	New	0:2	arg1	nanocomposites					49:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	5	24	theme	antimicrobial	1222:1234	arg1	activity					1236:1243	slightly higher antimicrobial activity	1206:1243	slightly higher antimicrobial activity	1206:1243	The Trp-TOC showed slightly higher antimicrobial activity than Phe-TOC especially in time required of killing performance.
31862374	1	25	theme	oxidized	163:170	arg1	l-tryptophan					253:264	l-tryptophan	253:264	l-tryptophan (Trp)	253:270	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	25	theme	oxidized	163:170	arg1	acids					219:223	oxidized cellulose (extracted from bagasse) (TOC) amino acids	163:223	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	25	theme	oxidized	163:170	arg1	alanine					235:241	l-phenyl alanine	226:241	l-phenyl alanine (Phe)	226:247	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	0	26	theme	potential	4:12	arg1	nanocomposites					49:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	6	27	theme	microbial	1356:1364	arg1	population					1366:1375	all microbial population	1352:1375	all microbial population	1352:1375	The Phe-TOC has required 20 h for killing all microbial population while Trp-TOC required only 12 h.
31862374	2	28	theme	spherical	614:622	arg1	shape					624:628	spherical shape	614:628	spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively	614:761	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	7	29	from	close	1431:1435	arg1	nanocomposites					1445:1458	both nanocomposites	1440:1458	both nanocomposites	1440:1458	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	2	30	theme	l-phenylalanine	677:691	arg1	Phe-TOC					704:710	Phe-TOC	704:710	Phe-TOC	704:710	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	30	theme	l-phenylalanine	677:691	arg1	composite					693:701	l-phenylalanine composite	677:701	l-phenylalanine composite (Phe-TOC)	677:711	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	31	theme	cellulose	172:180	arg1	l-tryptophan					253:264	l-tryptophan	253:264	l-tryptophan (Trp)	253:270	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	31	theme	cellulose	172:180	arg1	acids					219:223	oxidized cellulose (extracted from bagasse) (TOC) amino acids	163:223	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	31	theme	cellulose	172:180	arg1	alanine					235:241	l-phenyl alanine	226:241	l-phenyl alanine (Phe)	226:247	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	2	32	theme	FT-IR	417:421	arg1	spectroscopy					424:435	Infrared (FT-IR) spectroscopy	407:435	Infrared (FT-IR) spectroscopy	407:435	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	7	33	theme	Trp-TOC	1535:1541	arg1	case					1527:1530	the case	1523:1530	the case of Trp-TOC	1523:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	2	34	theme	l-tryptophan	717:728	arg1	Trp-TOC					741:747	Trp-TOC	741:747	Trp-TOC	741:747	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	34	theme	l-tryptophan	717:728	arg1	composite					730:738	l-tryptophan composite	717:738	l-tryptophan composite (Trp-TOC)	717:748	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	0	35	theme	green	14:18	arg1	nanocomposites					49:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	3	36	theme	unicellular	1011:1021	arg1	fungi					1023:1027	(iii) unicellular fungi	1005:1027	(iii) unicellular fungi	1005:1027	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	4	37	theme	broad-spectrum	1148:1161	arg1	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	2	38	dep	approved	555:562	arg1	Fourier					389:395	Fourier	389:395	Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively	389:761	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	7	39	with	close	1431:1435	arg1	measurements					1481:1492	high clear zone measurements	1465:1492	high clear zone measurements in the same concentration in the case of Trp-TOC	1465:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	4	40	contain	have	1117:1120	arg2	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	4	40	contain	have	1117:1120	arg1	composites					1106:1115	the both composites	1097:1115	the both composites	1097:1115	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	5	41	theme	higher	1215:1220	arg1	activity					1236:1243	slightly higher antimicrobial activity	1206:1243	slightly higher antimicrobial activity	1206:1243	The Trp-TOC showed slightly higher antimicrobial activity than Phe-TOC especially in time required of killing performance.
31862374	1	42	dep	cellulose	172:180	arg1	extracted					183:191	extracted	183:191	extracted from bagasse	183:204	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	7	43	theme	MIC	1415:1417	arg1	close					1431:1435	close	1431:1435	close	1431:1435	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	7	43	theme	MIC	1415:1417	arg1	values					1419:1424	The MIC values	1411:1424	The MIC values	1411:1424	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	0	44	theme	bioactive	21:29	arg1	nanocomposites					49:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	2	45	theme	thermal	438:444	arg1	analysis					446:453	thermal analysis	438:453	thermal analysis (TGA and DTGA)	438:468	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	3	46	dep	bacteria	828:835	arg1	i					811:811	i	811:811	i	811:811	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	3	47	dep	fungi	1023:1027	arg1	iii					1006:1008	iii	1006:1008	iii	1006:1008	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	2	48	theme	electron	523:530	arg1	TEM					544:546	TEM	544:546	TEM	544:546	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	48	theme	electron	523:530	arg1	microscope					532:541	transmission electron microscope	510:541	transmission electron microscope (TEM)	510:547	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	4	49	theme	antimicrobial	1163:1175	arg1	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	2	50	theme	scanning	471:478	arg1	SEM					500:502	SEM	500:502	SEM	500:502	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	50	theme	scanning	471:478	arg1	microscope					489:498	scanning electron microscope	471:498	scanning electron microscope(SEM)	471:503	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	51	theme	particle	643:650	arg1	size					652:655	average particle size 72	635:658	average particle size 72	635:658	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	52	theme	present	103:109	arg1	study					111:115	The present study	99:115	The present study	99:115	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	0	53	theme	antimicrobial	35:47	arg1	nanocomposites					49:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites	0:62	New potential green, bioactive and antimicrobial nanocomposites based on cellulose and amino acid.
31862374	2	54	theme	transmission	510:521	arg1	TEM					544:546	TEM	544:546	TEM	544:546	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	54	theme	transmission	510:521	arg1	microscope					532:541	transmission electron microscope	510:541	transmission electron microscope (TEM)	510:547	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	4	55	theme	effective	1127:1135	arg1	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	2	56	dep	Fourier	389:395	arg1	transform					397:405	transform	397:405	transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM)	397:547	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	57	theme	average	635:641	arg1	size					652:655	average particle size 72	635:658	average particle size 72	635:658	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	58	from	synthesis	573:581	arg1	nano-scale					600:609	nano-scale	600:609	nano-scale	600:609	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	6	59	theme	only	1400:1403	arg1	12 h					1405:1408	only 12 h	1400:1408	only 12 h	1400:1408	The Phe-TOC has required 20 h for killing all microbial population while Trp-TOC required only 12 h.
31862374	7	60	theme	same	1501:1504	arg1	concentration					1506:1518	the same concentration	1497:1518	the same concentration in the case of Trp-TOC	1497:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	7	61	from	measurements	1481:1492	arg1	concentration					1506:1518	the same concentration	1497:1518	the same concentration in the case of Trp-TOC	1497:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	4	62	theme	high	1122:1125	arg1	activity					1177:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	high effective, rapid and broad-spectrum antimicrobial activity	1122:1184	The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity.
31862374	3	63	dep	coli	850:853	arg1	bacteria					927:934	Gram-positive bacteria	913:934	Gram-positive bacteria	913:934	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	3	63	dep	coli	850:853	arg1	ii					909:910	ii	909:910	ii	909:910	The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11).
31862374	5	64	theme	killing	1289:1295	arg1	performance					1297:1307	killing performance	1289:1307	killing performance	1289:1307	The Trp-TOC showed slightly higher antimicrobial activity than Phe-TOC especially in time required of killing performance.
31862374	1	65	dep	acids	219:223	arg1	acids					219:223	oxidized cellulose (extracted from bagasse) (TOC) amino acids	163:223	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	65	dep	acids	219:223	arg1	Trp					267:269	Trp	267:269	Trp	267:269	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	65	dep	acids	219:223	arg1	Phe					244:246	Phe	244:246	Phe	244:246	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	65	dep	acids	219:223	arg1	l-tryptophan					253:264	l-tryptophan	253:264	l-tryptophan (Trp)	253:270	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	65	dep	acids	219:223	arg1	alanine					235:241	l-phenyl alanine	226:241	l-phenyl alanine (Phe)	226:247	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	2	66	from	nano-scale	600:609	arg1	synthesis					573:581	the synthesis	569:581	the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively	569:761	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	2	66	from	nano-scale	600:609	arg1	shape					624:628	spherical shape	614:628	spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively	614:761	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	7	67	from	concentration	1506:1518	arg1	case					1527:1530	the case	1523:1530	the case of Trp-TOC	1523:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	1	68	theme	novel	128:132	arg1	method					147:152	novel synthesizing method	128:152	novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	128:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	7	69	theme	clear	1470:1474	arg1	measurements					1481:1492	high clear zone measurements	1465:1492	high clear zone measurements in the same concentration in the case of Trp-TOC	1465:1541	The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
31862374	2	70	theme	Infrared	407:414	arg1	spectroscopy					424:435	Infrared (FT-IR) spectroscopy	407:435	Infrared (FT-IR) spectroscopy	407:435	The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively.
31862374	1	71	theme	synthesizing	134:145	arg1	method					147:152	novel synthesizing method	128:152	novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	128:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	72	theme	amino	213:217	arg1	l-tryptophan					253:264	l-tryptophan	253:264	l-tryptophan (Trp)	253:270	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	72	theme	amino	213:217	arg1	acids					219:223	oxidized cellulose (extracted from bagasse) (TOC) amino acids	163:223	TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites	157:287	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
31862374	1	72	theme	amino	213:217	arg1	alanine					235:241	l-phenyl alanine	226:241	l-phenyl alanine (Phe)	226:247	The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents.
30823358	0	0	theme	Hydroxyapatite/Chitosan	68:90	arg1	Composite					92:100	Nano Hydroxyapatite/Chitosan Composite	63:100	Nano Hydroxyapatite/Chitosan Composite	63:100	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	0	0	theme	Hydroxyapatite/Chitosan	68:90	arg1	Sorbent					107:113	a Sorbent	105:113	a Sorbent	105:113	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	7	1	theme	isotherm	1046:1053	arg1	model					1055:1059	The isotherm model	1042:1059	The isotherm model	1042:1059	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	4	2	theme	TEM	661:663	arg1	analysis					665:672	TEM analysis	661:672	TEM analysis	661:672	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	1	3	theme	in	242:243	arg1	method					264:269	an in situ precipitation method	239:269	an in situ precipitation method	239:269	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	0	4	theme	Nano	63:66	arg1	Composite					92:100	Nano Hydroxyapatite/Chitosan Composite	63:100	Nano Hydroxyapatite/Chitosan Composite	63:100	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	0	4	theme	Nano	63:66	arg1	Sorbent					107:113	a Sorbent	105:113	a Sorbent	105:113	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	5	5	theme	pH	740:741	arg1	dosage					810:815	the adsorbent dosage and zero point	796:830	dosage	810:815	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	5	theme	pH	740:741	arg1	effects					729:735	the effects	725:735	the effects of pH, contact time, and initial concentration	725:782	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	5	theme	pH	740:741	arg1	point					826:830	the adsorbent dosage and zero point	796:830	point	826:830	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	6	6	theme	reaction	1026:1033	arg1	order					1035:1039	reaction order	1026:1039	reaction order	1026:1039	The sorption models using Mories-Weber, Lagrange, and Bangham equations were used to identify the mechanism and reaction order.
30823358	8	7	theme	entropy	1259:1265	arg1	∆So					1275:1277	∆So	1275:1277	∆So	1275:1277	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	8	7	theme	entropy	1259:1265	arg1	change					1267:1272	entropy change	1259:1272	entropy change (∆So)	1259:1278	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	9	8	theme	green	1419:1423	arg1	removal					1429:1435	brilliant green dye removal	1409:1435	brilliant green dye removal	1409:1435	All of the results suggest the feasibility of using nanocomposites as a sorbent for brilliant green dye removal.
30823358	3	9	theme	contaminated	486:497	arg1	solution					505:512	a contaminated water solution	484:512	a contaminated water solution	484:512	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	1	10	theme	precipitation	250:262	arg1	method					264:269	an in situ precipitation method	239:269	an in situ precipitation method	239:269	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	5	11	theme	adsorption	894:903	arg1	process					905:911	the adsorption process	890:911	the adsorption process	890:911	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	2	12	theme	cost-effective	311:324	arg1	precursors					326:335	cost-effective precursors	311:335	cost-effective precursors within chitosan (CS) dissolved in aqueous acetic acid solution	311:398	Hydroxyapatite (Hap) was prepared from cost-effective precursors within chitosan (CS) dissolved in aqueous acetic acid solution.
30823358	6	13	dep	Mories-Weber	940:951	arg1	equations					976:984	equations	976:984	equations	976:984	The sorption models using Mories-Weber, Lagrange, and Bangham equations were used to identify the mechanism and reaction order.
30823358	3	14	theme	green	464:468	arg1	BG					475:476	BG	475:476	BG	475:476	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	3	14	theme	green	464:468	arg1	dye					470:472	brilliant green dye	454:472	brilliant green dye (BG)	454:477	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	2	15	theme	acid	386:389	arg1	solution					391:398	aqueous acetic acid solution	371:398	aqueous acetic acid solution	371:398	Hydroxyapatite (Hap) was prepared from cost-effective precursors within chitosan (CS) dissolved in aqueous acetic acid solution.
30823358	7	16	theme	Dubinin-Radusekevisch-Kanager	1109:1137	arg1	equations					1139:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	4	17	theme	nanocomposite	569:581	arg1	properties					551:560	The compositional and morphological properties	515:560	The compositional and morphological properties of the nanocomposite	515:581	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	6	18	used	used	991:994	arg2	models					927:932	The sorption models	914:932	The sorption models using Mories-Weber, Lagrange, and Bangham equations	914:984	The sorption models using Mories-Weber, Lagrange, and Bangham equations were used to identify the mechanism and reaction order.
30823358	2	19	theme	acetic	379:384	arg1	solution					391:398	aqueous acetic acid solution	371:398	aqueous acetic acid solution	371:398	Hydroxyapatite (Hap) was prepared from cost-effective precursors within chitosan (CS) dissolved in aqueous acetic acid solution.
30823358	7	20	theme	Freundlich	1093:1102	arg1	equations					1139:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	7	21	theme	adsorption	1198:1207	arg1	type					1190:1193	type	1190:1193	type	1190:1193	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	7	21	theme	adsorption	1198:1207	arg1	capacity					1177:1184	adsorption capacity	1166:1184	adsorption capacity	1166:1184	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	8	22	theme	Gibbs	1285:1289	arg1	∆Go					1304:1306	∆Go	1304:1306	∆Go	1304:1306	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	8	22	theme	Gibbs	1285:1289	arg1	energy					1296:1301	Gibbs free energy	1285:1301	Gibbs free energy (∆Go)	1285:1307	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	2	23	theme	aqueous	371:377	arg1	solution					391:398	aqueous acetic acid solution	371:398	aqueous acetic acid solution	371:398	Hydroxyapatite (Hap) was prepared from cost-effective precursors within chitosan (CS) dissolved in aqueous acetic acid solution.
30823358	4	24	theme	morphological	537:549	arg1	properties					551:560	The compositional and morphological properties	515:560	The compositional and morphological properties of the nanocomposite	515:581	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	5	25	theme	suitable	870:877	arg1	medium					879:884	a suitable medium	868:884	a suitable medium for the adsorption process	868:911	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	0	26	theme	Brilliant	15:23	arg1	Dye					31:33	Brilliant Green Dye	15:33	Brilliant Green Dye	15:33	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	3	27	theme	brilliant	454:462	arg1	BG					475:476	BG	475:476	BG	475:476	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	3	27	theme	brilliant	454:462	arg1	dye					470:472	brilliant green dye	454:472	brilliant green dye (BG)	454:477	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	5	28	theme	concentration	770:782	arg1	dosage					810:815	the adsorbent dosage and zero point	796:830	dosage	810:815	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	28	theme	concentration	770:782	arg1	effects					729:735	the effects	725:735	the effects of pH, contact time, and initial concentration	725:782	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	28	theme	concentration	770:782	arg1	point					826:830	the adsorbent dosage and zero point	796:830	point	826:830	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	8	29	theme	free	1291:1294	arg1	∆Go					1304:1306	∆Go	1304:1306	∆Go	1304:1306	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	8	29	theme	free	1291:1294	arg1	energy					1296:1301	Gibbs free energy	1285:1301	Gibbs free energy (∆Go)	1285:1307	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	1	30	theme	composition	188:198	arg1	terms					174:178	terms	174:178	terms of both composition and microstructure	174:217	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	0	31	theme	Dye	31:33	arg1	Removal					4:10	The Removal	0:10	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.	0:114	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	5	32	theme	Batch	675:679	arg1	experiments					681:691	Batch experiments	675:691	Batch experiments	675:691	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	33	dep	dosage	810:815	arg1	charge					832:837	charge	832:837	the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process	796:911	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	9	34	theme	dye	1425:1427	arg1	removal					1429:1435	brilliant green dye removal	1409:1435	brilliant green dye removal	1409:1435	All of the results suggest the feasibility of using nanocomposites as a sorbent for brilliant green dye removal.
30823358	0	35	theme	Green	25:29	arg1	Dye					31:33	Brilliant Green Dye	15:33	Brilliant Green Dye	15:33	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	7	36	dep	capacity	1177:1184	arg1	the					1162:1164	the	1162:1164	the	1162:1164	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	5	37	theme	initial	762:768	arg1	concentration					770:782	initial concentration	762:782	initial concentration	762:782	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	1	38	theme	microstructure	204:217	arg1	terms					174:178	terms	174:178	terms of both composition and microstructure	174:217	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	0	39	theme	Aqueous	40:46	arg1	Solution					48:55	Aqueous Solution	40:55	Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent	40:113	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	0	40	from	Solution	48:55	arg1	Removal					4:10	The Removal	0:10	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.	0:114	The Removal of Brilliant Green Dye from Aqueous Solution Using Nano Hydroxyapatite/Chitosan Composite as a Sorbent.
30823358	5	41	theme	time	752:755	arg1	dosage					810:815	the adsorbent dosage and zero point	796:830	dosage	810:815	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	41	theme	time	752:755	arg1	effects					729:735	the effects	725:735	the effects of pH, contact time, and initial concentration	725:782	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	5	41	theme	time	752:755	arg1	point					826:830	the adsorbent dosage and zero point	796:830	point	826:830	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	4	42	theme	compositional	519:531	arg1	properties					551:560	The compositional and morphological properties	515:560	The compositional and morphological properties of the nanocomposite	515:581	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	9	43	theme	brilliant	1409:1417	arg1	removal					1429:1435	brilliant green dye removal	1409:1435	brilliant green dye removal	1409:1435	All of the results suggest the feasibility of using nanocomposites as a sorbent for brilliant green dye removal.
30823358	8	44	theme	enthalpy	1236:1243	arg1	∆Ho					1253:1255	∆Ho	1253:1255	∆Ho	1253:1255	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	8	44	theme	enthalpy	1236:1243	arg1	change					1245:1250	enthalpy change	1236:1250	enthalpy change (∆Ho)	1236:1256	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	7	45	theme	Langmuir	1083:1090	arg1	equations					1139:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations	1083:1147	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	3	46	theme	dye	470:472	arg1	removal					443:449	the removal	439:449	the removal of brilliant green dye (BG) from a contaminated water solution	439:512	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	6	47	theme	sorption	918:925	arg1	models					927:932	The sorption models	914:932	The sorption models using Mories-Weber, Lagrange, and Bangham equations	914:984	The sorption models using Mories-Weber, Lagrange, and Bangham equations were used to identify the mechanism and reaction order.
30823358	1	48	dep	in	242:243	arg1	situ					245:248	situ	245:248	situ	245:248	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	1	49	theme	natural	134:140	arg1	bone					142:145	natural bone	134:145	natural bone	134:145	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	7	50	theme	adsorption	1166:1175	arg1	capacity					1177:1184	adsorption capacity	1166:1184	adsorption capacity	1166:1184	The isotherm model was carried out using Langmuir, Freundlich, and Dubinin-Radusekevisch-Kanager equations to calculate the adsorption capacity and type of adsorption.
30823358	4	51	theme	X-ray	627:631	arg1	XRD					646:648	XRD	646:648	XRD	646:648	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	4	51	theme	X-ray	627:631	arg1	diffraction					633:643	X-ray diffraction	627:643	X-ray diffraction (XRD)	627:649	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	8	52	theme	Thermodynamic	1210:1222	arg1	parameters					1224:1233	Thermodynamic parameters	1210:1233	Thermodynamic parameters	1210:1233	Thermodynamic parameters, enthalpy change (∆Ho), entropy change (∆So), and Gibbs free energy (∆Go) were evaluated.
30823358	1	53	theme	bone	142:145	arg1	Nanocomposites					116:129	Nanocomposites	116:129	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure	116:217	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	1	53	theme	bone	142:145	arg1	bone					142:145	natural bone	134:145	natural bone	134:145	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	5	54	theme	adsorbent	800:808	arg1	dosage					810:815	the adsorbent dosage and zero point	796:830	dosage	810:815	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	4	55	theme	FTIR	608:611	arg1	spectroscopy					613:624	FTIR spectroscopy	608:624	FTIR spectroscopy	608:624	The compositional and morphological properties of the nanocomposite were studied by means of FTIR spectroscopy, X-ray diffraction (XRD), SEM, and TEM analysis.
30823358	5	56	theme	contact	744:750	arg1	time					752:755	contact time	744:755	contact time	744:755	Batch experiments were carried out to investigate the effects of pH, contact time, and initial concentration, as well as the adsorbent dosage and zero point charge for the sorbent to determine a suitable medium for the adsorption process.
30823358	1	57	from	benefits	162:169	arg1	terms					174:178	terms	174:178	terms of both composition and microstructure	174:217	Nanocomposites of natural bone that show some benefits in terms of both composition and microstructure were synthesized by an in situ precipitation method.
30823358	3	58	theme	water	499:503	arg1	solution					505:512	a contaminated water solution	484:512	a contaminated water solution	484:512	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
30823358	3	59	from	solution	505:512	arg1	removal					443:449	the removal	439:449	the removal of brilliant green dye (BG) from a contaminated water solution	439:512	The nanocomposite was synthesized for the removal of brilliant green dye (BG) from a contaminated water solution.
31517429	8	0	theme	molecular	1152:1160	arg1	weight					1162:1167	an molecular weight	1149:1167	an molecular weight of approximately 2·5 × 106 Da	1149:1197	: 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da.
31517429	13	1	from	ZCC3656	1629:1635	arg1	Riclin					1617:1622	The EPS Riclin	1609:1622	The EPS Riclin from ZCC3656	1609:1635	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	1	from	ZCC3656	1629:1635	arg1	polysaccharide					1661:1674	a succinoglycan-type polysaccharide	1640:1674	a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro	1640:1766	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	1	from	ZCC3656	1629:1635	arg1	noncytotoxic					1684:1695	noncytotoxic	1684:1695	noncytotoxic	1684:1695	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	15	2	theme	little	1981:1986	arg1	attention					1988:1996	little attention	1981:1996	little attention	1981:1996	However, their potential activity towards mammals has received little attention.
31517429	6	3	theme	Sevag	829:833	arg1	method					835:840	the Sevag method	825:840	the Sevag method	825:840	The EPS, designated Riclin, was purified by deproteinization using the Sevag method.
31517429	2	4	theme	liver	353:357	arg1	model					372:376	an acute liver injury mouse model	344:376	an acute liver injury mouse model	344:376	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	7	5	theme	chromatography	882:895	arg1	analyses					978:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	5	6	theme	carbon	672:677	arg1	utilization					686:696	carbon source utilization	672:696	carbon source utilization	672:696	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	11	7	theme	Agrobacterium	1542:1554	arg1	sp					1556:1557	Agrobacterium sp	1542:1557	Agrobacterium sp	1542:1557	CONCLUSIONS Agrobacterium sp.
31517429	7	8	theme	gel	867:869	arg1	chromatography					882:895	gel permeation chromatography	867:895	gel permeation chromatography	867:895	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	2	9	from	ZCC3656	194:200	arg1	EPS					160:162	EPS	160:162	EPS	160:162	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	2	9	from	ZCC3656	194:200	arg1	exopolysaccharide					141:157	an exopolysaccharide	138:157	an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance	138:236	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	10	10	theme	AST	1435:1437	arg1	activities					1439:1448	serum ALT and AST activities	1421:1448	serum ALT and AST activities	1421:1448	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	3	11	theme	carbon	541:546	arg1	source					548:553	the sole carbon source	532:553	the sole carbon source	532:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	9	12	theme	IL-1β	1224:1228	arg1	expression					1239:1248	TNF-α, IL-1β and IL-6 expression	1217:1248	TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells	1217:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	2	13	theme	-induced	315:322	arg1	cells					335:339	lipopolysaccharide (LPS)-induced macrophage cells	291:339	lipopolysaccharide (LPS)-induced macrophage cells	291:339	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	5	14	theme	highest	596:602	arg1	yield					608:612	the highest EPS yield	592:612	the highest EPS yield	592:612	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	9	15	theme	IL-6	1234:1237	arg1	expression					1239:1248	TNF-α, IL-1β and IL-6 expression	1217:1248	TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells	1217:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	5	16	theme	serial	728:733	arg1	assays					750:755	serial subcultivation assays	728:755	serial subcultivation assays	728:755	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	2	17	theme	EPS-secreting	212:224	arg1	performance					226:236	high EPS-secreting performance	207:236	high EPS-secreting performance	207:236	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	12	18	theme	EPS-producing	1587:1599	arg1	ZCC3656					1560:1566	ZCC3656	1560:1566	ZCC3656	1560:1566	ZCC3656 is a highly stable EPS-producing strain.
31517429	12	18	theme	EPS-producing	1587:1599	arg1	strain					1601:1606	a highly stable EPS-producing strain	1571:1606	a highly stable EPS-producing strain	1571:1606	ZCC3656 is a highly stable EPS-producing strain.
31517429	9	19	theme	RAW	1268:1270	arg1	cells					1289:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	7	20	theme	magnetic	959:966	arg1	resonance					968:976	nuclear magnetic resonance	951:976	nuclear magnetic resonance	951:976	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	5	21	theme	time	699:702	arg1	course					704:709	time course	699:709	time course fermentation	699:722	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	9	22	theme	macrophage	1278:1287	arg1	cells					1289:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	0	23	theme	a	51:51	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity of a	0:51	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	14	24	theme	physiological	1878:1890	arg1	interactions					1892:1903	their physiological interactions	1872:1903	their physiological interactions with plants	1872:1915	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	9	25	theme	dose-dependent	1300:1313	arg1	manner					1315:1320	a dose-dependent manner	1298:1320	a dose-dependent manner	1298:1320	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	9	26	theme	TNF-α	1217:1221	arg1	expression					1239:1248	TNF-α, IL-1β and IL-6 expression	1217:1248	TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells	1217:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	10	27	theme	mice	1405:1408	arg1	rate					1379:1382	the survival rate	1366:1382	the survival rate of D-Gal/LPS treated mice	1366:1408	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	0	28	theme	Agrobacterium	79:91	arg1	sp					93:94	Agrobacterium sp	79:94	Agrobacterium sp	79:94	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	16	29	theme	succinoglycan	2027:2039	arg1	reagent					2136:2142	a promising reagent	2124:2142	a promising reagent in anti-inflammatory treatment	2124:2173	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	16	29	theme	succinoglycan	2027:2039	arg1	Riclin					2041:2046	the succinoglycan Riclin	2023:2046	the succinoglycan Riclin	2023:2046	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	10	30	theme	inflammatory	1484:1495	arg1	IL-6					1524:1527	IL-6	1524:1527	IL-6	1524:1527	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	30	theme	inflammatory	1484:1495	arg1	mediators					1497:1505	the inflammatory mediators TNF-α, IL-1β and IL-6	1480:1527	the inflammatory mediators TNF-α, IL-1β and IL-6	1480:1527	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	30	theme	inflammatory	1484:1495	arg1	IL-1β					1514:1518	IL-1β	1514:1518	IL-1β	1514:1518	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	30	theme	inflammatory	1484:1495	arg1	TNF-α					1507:1511	TNF-α	1507:1511	TNF-α	1507:1511	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	3	31	theme	EPS	442:444	arg1	production					459:468	EPS fermentation production	442:468	EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	442:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	7	32	theme	resonance	968:976	arg1	analyses					978:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	13	33	theme	anti-inflammatory	1721:1737	arg1	effects					1739:1745	remarkable anti-inflammatory effects	1710:1745	remarkable anti-inflammatory effects	1710:1745	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	0	34	theme	In	0:1	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity of a	0:51	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	7	35	theme	nuclear	951:957	arg1	resonance					968:976	nuclear magnetic resonance	951:976	nuclear magnetic resonance	951:976	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	16	36	theme	anti-inflammatory	2068:2084	arg1	activities					2086:2095	excellent anti-inflammatory activities	2058:2095	excellent anti-inflammatory activities	2058:2095	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	4	37	theme	Agrobacterium	556:568	arg1	sp					570:571	Agrobacterium sp	556:571	Agrobacterium sp.	556:572	Agrobacterium sp.
31517429	3	38	theme	modified	473:480	arg1	salts					485:489	modified M9 salts	473:489	modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	473:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	14	39	theme	good	1840:1843	arg1	properties					1857:1866	good rheological properties	1840:1866	good rheological properties	1840:1866	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	2	40	theme	acute	347:351	arg1	model					372:376	an acute liver injury mouse model	344:376	an acute liver injury mouse model	344:376	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	13	41	theme	succinoglycan-type	1642:1659	arg1	Riclin					1617:1622	The EPS Riclin	1609:1622	The EPS Riclin from ZCC3656	1609:1635	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	41	theme	succinoglycan-type	1642:1659	arg1	polysaccharide					1661:1674	a succinoglycan-type polysaccharide	1640:1674	a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro	1640:1766	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	41	theme	succinoglycan-type	1642:1659	arg1	noncytotoxic					1684:1695	noncytotoxic	1684:1695	noncytotoxic	1684:1695	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	2	42	theme	strain	187:192	arg1	ZCC3656					194:200	an Agrobacterium strain ZCC3656	170:200	an Agrobacterium strain ZCC3656	170:200	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	10	43	theme	Riclin	1336:1341	arg1	pretreatment					1343:1354	Riclin pretreatment	1336:1354	Riclin pretreatment	1336:1354	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	14	44	theme	STUDY	1800:1804	arg1	Succinoglycans					1806:1819	THE STUDY Succinoglycans	1796:1819	THE STUDY Succinoglycans	1796:1819	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	7	45	theme	methylation	926:936	arg1	analysis					938:945	methylation analysis	926:945	methylation analysis	926:945	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	0	46	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	5	47	theme	EPS	654:656	arg1	production					658:667	EPS production	654:667	EPS production	654:667	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	10	48	theme	survival	1370:1377	arg1	rate					1379:1382	the survival rate	1366:1382	the survival rate of D-Gal/LPS treated mice	1366:1408	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	2	49	theme	injury	359:364	arg1	model					372:376	an acute liver injury mouse model	344:376	an acute liver injury mouse model	344:376	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	7	50	theme	composition	913:923	arg1	analyses					978:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	5	51	dep	exhibited	582:590	arg1	21·1 g l-1					615:624	21·1 g l-1	615:624	21·1 g l-1	615:624	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	13	52	theme	EPS	1613:1615	arg1	Riclin					1617:1622	The EPS Riclin	1609:1622	The EPS Riclin from ZCC3656	1609:1635	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	52	theme	EPS	1613:1615	arg1	polysaccharide					1661:1674	a succinoglycan-type polysaccharide	1640:1674	a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro	1640:1766	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	13	52	theme	EPS	1613:1615	arg1	noncytotoxic					1684:1695	noncytotoxic	1684:1695	noncytotoxic	1684:1695	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	14	53	with	interactions	1892:1903	arg1	plants					1910:1915	plants	1910:1915	plants	1910:1915	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	10	54	theme	treated	1397:1403	arg1	mice					1405:1408	D-Gal/LPS treated mice	1387:1408	D-Gal/LPS treated mice	1387:1408	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	3	55	theme	sole	536:539	arg1	source					548:553	the sole carbon source	532:553	the sole carbon source	532:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	7	56	theme	permeation	871:880	arg1	chromatography					882:895	gel permeation chromatography	867:895	gel permeation chromatography	867:895	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	9	57	from	expression	1239:1248	arg1	cells					1289:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	7	58	theme	7·8	1118:1120	arg1	ratio					1109:1113	a ratio	1107:1113	a ratio of 7·8	1107:1120	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	2	59	theme	macrophage	324:333	arg1	cells					335:339	lipopolysaccharide (LPS)-induced macrophage cells	291:339	lipopolysaccharide (LPS)-induced macrophage cells	291:339	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	14	60	with	properties	1857:1866	arg1	plants					1910:1915	plants	1910:1915	plants	1910:1915	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	8	61	theme	Da	1196:1197	arg1	weight					1162:1167	an molecular weight	1149:1167	an molecular weight of approximately 2·5 × 106 Da	1149:1197	: 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da.
31517429	10	62	theme	ALT	1427:1429	arg1	activities					1439:1448	serum ALT and AST activities	1421:1448	serum ALT and AST activities	1421:1448	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	5	63	theme	course	704:709	arg1	fermentation					711:722	time course fermentation	699:722	time course fermentation	699:722	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	10	64	theme	serum	1421:1425	arg1	ALT					1427:1429	serum ALT	1421:1429	serum ALT	1421:1429	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	7	65	theme	combined	847:854	arg1	results					856:862	The combined results	843:862	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	843:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	15	66	theme	potential	1933:1941	arg1	activity					1943:1950	their potential activity	1927:1950	their potential activity towards mammals	1927:1966	However, their potential activity towards mammals has received little attention.
31517429	5	67	theme	source	679:684	arg1	utilization					686:696	carbon source utilization	672:696	carbon source utilization	672:696	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	9	68	theme	LPS-stimulated	1253:1266	arg1	cells					1289:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	LPS-stimulated RAW 264.7 macrophage cells	1253:1293	Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner.
31517429	2	69	theme	high	207:210	arg1	performance					226:236	high EPS-secreting performance	207:236	high EPS-secreting performance	207:236	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	16	70	theme	anti-inflammatory	2147:2163	arg1	treatment					2165:2173	anti-inflammatory treatment	2147:2173	anti-inflammatory treatment	2147:2173	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	2	71	theme	anti-inflammatory	258:274	arg1	activity					276:283	its anti-inflammatory activity	254:283	its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells	254:339	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	0	72	from	sp	93:94	arg1	Riclin					67:72	Riclin	67:72	Riclin from Agrobacterium sp	67:94	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	12	73	theme	stable	1580:1585	arg1	ZCC3656					1560:1566	ZCC3656	1560:1566	ZCC3656	1560:1566	ZCC3656 is a highly stable EPS-producing strain.
31517429	12	73	theme	stable	1580:1585	arg1	strain					1601:1606	a highly stable EPS-producing strain	1571:1606	a highly stable EPS-producing strain	1571:1606	ZCC3656 is a highly stable EPS-producing strain.
31517429	10	74	dep	mediators	1497:1505	arg1	IL-6					1524:1527	IL-6	1524:1527	IL-6	1524:1527	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	74	dep	mediators	1497:1505	arg1	mediators					1497:1505	the inflammatory mediators TNF-α, IL-1β and IL-6	1480:1527	the inflammatory mediators TNF-α, IL-1β and IL-6	1480:1527	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	74	dep	mediators	1497:1505	arg1	IL-1β					1514:1518	IL-1β	1514:1518	IL-1β	1514:1518	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	10	74	dep	mediators	1497:1505	arg1	TNF-α					1507:1511	TNF-α	1507:1511	TNF-α	1507:1511	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	7	75	theme	succinoglycan-like	1014:1031	arg1	polysaccharide					1033:1046	a succinoglycan-like polysaccharide	1012:1046	a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8	1012:1120	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	7	75	theme	succinoglycan-like	1014:1031	arg1	Riclin					1002:1007	Riclin	1002:1007	Riclin	1002:1007	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	3	76	dep	METHODS	379:385	arg1	compared					429:436	compared	429:436	were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	424:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	5	77	theme	subcultivation	735:748	arg1	assays					750:755	serial subcultivation assays	728:755	serial subcultivation assays	728:755	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	16	78	theme	excellent	2058:2066	arg1	activities					2086:2095	excellent anti-inflammatory activities	2058:2095	excellent anti-inflammatory activities	2058:2095	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	13	79	theme	remarkable	1710:1719	arg1	effects					1739:1745	remarkable anti-inflammatory effects	1710:1745	remarkable anti-inflammatory effects	1710:1745	The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
31517429	3	80	theme	fermentation	446:457	arg1	production					459:468	EPS fermentation production	442:468	EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	442:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	7	81	theme	analyses	978:985	arg1	results					856:862	The combined results	843:862	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	843:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	0	82	theme	in	13:14	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity of a	0:51	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	14	83	theme	rheological	1845:1855	arg1	properties					1857:1866	good rheological properties	1840:1866	good rheological properties	1840:1866	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	5	84	theme	EPS	604:606	arg1	yield					608:612	the highest EPS yield	592:612	the highest EPS yield	592:612	ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays.
31517429	3	85	theme	M9	482:483	arg1	salts					485:489	modified M9 salts	473:489	modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	473:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	0	86	theme	anti-inflammatory	21:37	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity of a	0:51	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	2	87	with	exopolysaccharide	141:157	arg1	performance					226:236	high EPS-secreting performance	207:236	high EPS-secreting performance	207:236	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	10	88	theme	mediators	1497:1505	arg1	production					1466:1475	the production	1462:1475	the production of the inflammatory mediators TNF-α, IL-1β and IL-6	1462:1527	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	16	89	from	reagent	2136:2142	arg1	treatment					2165:2173	anti-inflammatory treatment	2147:2173	anti-inflammatory treatment	2147:2173	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	3	90	from	production	459:468	arg1	salts					485:489	modified M9 salts	473:489	modified M9 salts supplemented with mannitol or sucrose as the sole carbon source	473:553	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	8	91	theme	 0·9 	1130:1134	arg1	 1·0 					1124:1128	 1·0 	1124:1128	: 1·0 : 0·9 : 1·1	1123:1139	: 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da.
31517429	3	92	theme	rhizobial	406:414	arg1	strains					416:422	Twelve rhizobial strains	399:422	Twelve rhizobial strains	399:422	METHODS AND RESULTS Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source.
31517429	16	93	theme	promising	2126:2134	arg1	reagent					2136:2142	a promising reagent	2124:2142	a promising reagent in anti-inflammatory treatment	2124:2173	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	16	93	theme	promising	2126:2134	arg1	Riclin					2041:2046	the succinoglycan Riclin	2023:2046	the succinoglycan Riclin	2023:2046	Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
31517429	7	94	theme	analysis	938:945	arg1	analyses					978:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses	867:985	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31517429	14	95	theme	Succinoglycans	1806:1819	arg1	SIGNIFICANCE					1769:1780	SIGNIFICANCE	1769:1780	SIGNIFICANCE	1769:1780	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	14	95	theme	Succinoglycans	1806:1819	arg1	IMPACT					1786:1791	IMPACT	1786:1791	IMPACT	1786:1791	SIGNIFICANCE AND IMPACT OF THE STUDY Succinoglycans are well known for good rheological properties and their physiological interactions with plants.
31517429	0	96	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp.
31517429	2	97	theme	Agrobacterium	173:185	arg1	ZCC3656					194:200	an Agrobacterium strain ZCC3656	170:200	an Agrobacterium strain ZCC3656	170:200	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	10	98	theme	D-Gal/LPS	1387:1395	arg1	mice					1405:1408	D-Gal/LPS treated mice	1387:1408	D-Gal/LPS treated mice	1387:1408	In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
31517429	2	99	theme	mouse	366:370	arg1	model					372:376	an acute liver injury mouse model	344:376	an acute liver injury mouse model	344:376	AIMS To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
31517429	7	100	theme	monosaccharide	898:911	arg1	composition					913:923	monosaccharide composition	898:923	monosaccharide composition	898:923	The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8.
31199973	0	0	theme	Process	97:103	arg1	optimization					105:116	Process optimization	97:116	Process optimization	97:116	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	2	1	theme	concentration	624:636	arg1	%					643:643	(NH4)2SO4 concentration 19.58%	614:643	(NH4)2SO4 concentration 19.58% (w/w)	614:649	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	1	theme	concentration	624:636	arg1	w/w					646:648	w/w	646:648	w/w	646:648	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	1	2	theme	two-phase	199:207	arg1	MAATPE					221:226	MAATPE	221:226	MAATPE	221:226	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	2	theme	two-phase	199:207	arg1	extraction					209:218	Microwave-assisted aqueous two-phase extraction	172:218	Microwave-assisted aqueous two-phase extraction (MAATPE)	172:227	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	3	theme	Lentinus	300:307	arg1	edodes					309:314	Lentinus edodes	300:314	Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	300:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	6	4	theme	superoxide	1297:1306	arg1	radicals					1308:1315	scavenging DPPH, hydroxyl and superoxide radicals	1267:1315	radicals	1308:1315	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	6	5	theme	DPPH	1278:1281	arg1	radicals					1308:1315	scavenging DPPH, hydroxyl and superoxide radicals	1267:1315	radicals	1308:1315	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	2	6	theme	2SO4	619:622	arg1	%					643:643	(NH4)2SO4 concentration 19.58%	614:643	(NH4)2SO4 concentration 19.58% (w/w)	614:649	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	6	theme	2SO4	619:622	arg1	w/w					646:648	w/w	646:648	w/w	646:648	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	7	theme	temperature	663:673	arg1	78.7 °C					675:681	extraction temperature 78.7 °C	652:681	extraction temperature 78.7 °C	652:681	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	3	8	from	phase	801:805	arg1	yields					758:763	The extraction yields	743:763	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	743:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	8	from	phase	801:805	arg1	polysaccharides					772:786	the polysaccharides	768:786	the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	768:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	8	from	phase	801:805	arg1	%					856:856	2.12 ± 0.21%	845:856	2.12 ± 0.21%	845:856	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	4	9	theme	molecular	1015:1023	arg1	MW					1033:1034	MW	1033:1034	MW	1033:1034	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	4	9	theme	molecular	1015:1023	arg1	weight					1025:1030	their average molecular weight	1001:1030	their average molecular weight (MW)	1001:1035	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	7	10	theme	extraction	1548:1557	arg1	efficiency					1559:1568	extraction efficiency	1548:1568	extraction efficiency	1548:1568	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	2	11	theme	ethanol	576:582	arg1	%					602:602	ethanol concentration 26.0%	576:602	ethanol concentration 26.0% (w/w)	576:608	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	11	theme	ethanol	576:582	arg1	w/w					605:607	w/w	605:607	w/w	605:607	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	1	12	theme	biphasic	399:406	arg1	extractant					408:417	the biphasic extractant	395:417	the biphasic extractant	395:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	2	13	theme	extraction	652:661	arg1	78.7 °C					675:681	extraction temperature 78.7 °C	652:681	extraction temperature 78.7 °C	652:681	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	4	14	theme	average	1007:1013	arg1	MW					1033:1034	MW	1033:1034	MW	1033:1034	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	4	14	theme	average	1007:1013	arg1	weight					1025:1030	their average molecular weight	1001:1030	their average molecular weight (MW)	1001:1035	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	4	15	theme	HPLC	920:923	arg1	analysis					925:932	HPLC analysis	920:932	HPLC analysis	920:932	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	2	16	theme	NH4	615:617	arg1	%					643:643	(NH4)2SO4 concentration 19.58%	614:643	(NH4)2SO4 concentration 19.58% (w/w)	614:649	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	16	theme	NH4	615:617	arg1	w/w					646:648	w/w	646:648	w/w	646:648	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	7	17	theme	conventional	1463:1474	arg1	methods					1476:1482	conventional methods	1463:1482	conventional methods using a mono-phase solvent	1463:1509	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	1	18	theme	aqueous	321:327	arg1	extraction					339:348	aqueous two-phase extraction	321:348	aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	321:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	5	19	theme	process	1109:1115	arg1	mechanism					1089:1097	the mechanism	1085:1097	the mechanism of MAATPE process	1085:1115	Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy.
31199973	3	20	theme	extraction	747:756	arg1	yields					758:763	The extraction yields	743:763	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	743:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	20	theme	extraction	747:756	arg1	%					856:856	2.12 ± 0.21%	845:856	2.12 ± 0.21%	845:856	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	21	from	yields	758:763	arg1	PBP					835:837	PBP	835:837	PBP	835:837	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	21	from	yields	758:763	arg1	phase					828:832	the bottom phase	817:832	the bottom phase (PBP)	817:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	21	from	yields	758:763	arg1	PTP					808:810	PTP	808:810	PTP	808:810	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	21	from	yields	758:763	arg1	phase					801:805	the top phase	793:805	the top phase (PTP)	793:811	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	5	22	theme	scanning	1158:1165	arg1	microscopy					1176:1185	scanning electron microscopy	1158:1185	scanning electron microscopy	1158:1185	Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy.
31199973	0	23	theme	structure	119:127	arg1	characterization					129:144	structure characterization	119:144	structure characterization	119:144	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	5	24	theme	composition	1133:1143	arg1	analysis					1145:1152	composition analysis	1133:1152	composition analysis	1133:1152	Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy.
31199973	2	25	theme	time	695:698	arg1	19.55 min					700:708	extraction time 19.55 min	684:708	extraction time 19.55 min	684:708	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	6	26	theme	radicals	1308:1315	arg1	values					1257:1262	EC50 values	1252:1262	EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP	1252:1323	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	0	27	theme	aqueous	19:25	arg1	extraction					37:46	Microwave-assisted aqueous two-phase extraction	0:46	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.	0:170	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	2	28	theme	%	643:643	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	29	theme	extraction	684:693	arg1	19.55 min					700:708	extraction time 19.55 min	684:708	extraction time 19.55 min	684:708	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	5	30	theme	MAATPE	1102:1107	arg1	process					1109:1115	MAATPE process	1102:1115	MAATPE process	1102:1115	Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy.
31199973	2	31	theme	MAATPE	435:440	arg1	process					442:448	MAATPE process	435:448	MAATPE process	435:448	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	0	32	theme	Microwave-assisted	0:17	arg1	extraction					37:46	Microwave-assisted aqueous two-phase extraction	0:46	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.	0:170	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	1	33	theme	two-phase	329:337	arg1	extraction					339:348	aqueous two-phase extraction	321:348	aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	321:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	2	34	from	factors	424:430	arg1	process					442:448	MAATPE process	435:448	MAATPE process	435:448	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	6	35	theme	antioxidant	1228:1238	arg1	activities					1240:1249	stronger antioxidant activities	1219:1249	stronger antioxidant activities	1219:1249	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	2	36	theme	solid-to-solvent	714:729	arg1	ratio					731:735	solid-to-solvent ratio 1:50	714:740	solid-to-solvent ratio 1:50	714:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	6	37	theme	EC50	1252:1255	arg1	values					1257:1262	EC50 values	1252:1262	EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP	1252:1323	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	1	38	theme	extraction	339:348	arg1	system					350:355	aqueous two-phase extraction system	321:355	aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	321:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	38	theme	extraction	339:348	arg1	ATPS					358:361	ATPS	358:361	ATPS	358:361	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	0	39	theme	antioxidant	150:160	arg1	activity					162:169	antioxidant activity	150:169	antioxidant activity	150:169	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	2	40	theme	78.7 °C	675:681	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	3	41	theme	bottom	821:826	arg1	PBP					835:837	PBP	835:837	PBP	835:837	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	41	theme	bottom	821:826	arg1	phase					828:832	the bottom phase	817:832	the bottom phase (PBP)	817:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	2	42	theme	surface	477:483	arg1	methodology					485:495	response surface methodology	468:495	response surface methodology	468:495	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	0	43	theme	two-phase	27:35	arg1	extraction					37:46	Microwave-assisted aqueous two-phase extraction	0:46	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.	0:170	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	7	44	theme	mono-phase	1492:1501	arg1	solvent					1503:1509	a mono-phase solvent	1490:1509	a mono-phase solvent	1490:1509	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	2	45	theme	%	602:602	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	46	theme	response	468:475	arg1	methodology					485:495	response surface methodology	468:495	response surface methodology	468:495	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	0	47	theme	diverse	51:57	arg1	polysaccharides					59:73	diverse polysaccharides	51:73	diverse polysaccharides from Lentinus edodes	51:94	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	4	48	from	other	972:976	arg1	different					952:960	different	952:960	different	952:960	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	3	49	from	phase	828:832	arg1	yields					758:763	The extraction yields	743:763	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	743:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	49	from	phase	828:832	arg1	polysaccharides					772:786	the polysaccharides	768:786	the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	768:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	49	from	phase	828:832	arg1	%					856:856	2.12 ± 0.21%	845:856	2.12 ± 0.21%	845:856	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	1	50	theme	simultaneous	244:255	arg1	extraction					257:266	simultaneous extraction	244:266	simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	244:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	3	51	theme	top	797:799	arg1	PTP					808:810	PTP	808:810	PTP	808:810	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	51	theme	top	797:799	arg1	phase					801:805	the top phase	793:805	the top phase (PTP)	793:811	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	1	52	from	edodes	309:314	arg1	polysaccharides					279:293	diverse polysaccharides	271:293	diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	271:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	52	from	edodes	309:314	arg1	extraction					257:266	simultaneous extraction	244:266	simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	244:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	0	53	from	edodes	89:94	arg1	extraction					37:46	Microwave-assisted aqueous two-phase extraction	0:46	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.	0:170	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	0	53	from	edodes	89:94	arg1	polysaccharides					59:73	diverse polysaccharides	51:73	diverse polysaccharides from Lentinus edodes	51:94	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	2	54	dep	optimized	455:463	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	55	theme	concentration	584:596	arg1	%					602:602	ethanol concentration 26.0%	576:602	ethanol concentration 26.0% (w/w)	576:608	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	55	theme	concentration	584:596	arg1	w/w					605:607	w/w	605:607	w/w	605:607	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	56	theme	ATPS	556:559	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	7	57	theme	diverse	1600:1606	arg1	polysaccharides					1608:1622	diverse polysaccharides	1600:1622	diverse polysaccharides	1600:1622	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	3	58	theme	polysaccharides	772:786	arg1	yields					758:763	The extraction yields	743:763	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP)	743:838	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	3	58	theme	polysaccharides	772:786	arg1	%					856:856	2.12 ± 0.21%	845:856	2.12 ± 0.21%	845:856	The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively.
31199973	1	59	from	extraction	257:266	arg1	edodes					309:314	Lentinus edodes	300:314	Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	300:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	0	60	theme	polysaccharides	59:73	arg1	extraction					37:46	Microwave-assisted aqueous two-phase extraction	0:46	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.	0:170	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	4	61	from	different	952:960	arg1	structure					990:998	chemical structure	981:998	chemical structure	981:998	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	0	62	from	extraction	37:46	arg1	edodes					89:94	Lentinus edodes	80:94	Lentinus edodes	80:94	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	2	63	theme	optimum	506:512	arg1	conditions					514:523	the optimum conditions	502:523	the optimum conditions	502:523	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	6	64	theme	stronger	1219:1226	arg1	activities					1240:1249	stronger antioxidant activities	1219:1249	stronger antioxidant activities	1219:1249	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	1	65	theme	ethanol/ammonium	367:382	arg1	sulfate					384:390	ethanol/ammonium sulfate	367:390	ethanol/ammonium sulfate as the biphasic extractant	367:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	6	66	theme	scavenging	1267:1276	arg1	radicals					1308:1315	scavenging DPPH, hydroxyl and superoxide radicals	1267:1315	radicals	1308:1315	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	0	67	dep	extraction	37:46	arg1	activity					162:169	antioxidant activity	150:169	antioxidant activity	150:169	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	0	67	dep	extraction	37:46	arg1	optimization					105:116	Process optimization	97:116	Process optimization	97:116	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	0	67	dep	extraction	37:46	arg1	characterization					129:144	structure characterization	119:144	structure characterization	119:144	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	1	68	with	edodes	309:314	arg1	system					350:355	aqueous two-phase extraction system	321:355	aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	321:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	68	with	edodes	309:314	arg1	ATPS					358:361	ATPS	358:361	ATPS	358:361	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	6	69	theme	hydroxyl	1284:1291	arg1	radicals					1308:1315	scavenging DPPH, hydroxyl and superoxide radicals	1267:1315	radicals	1308:1315	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	5	70	theme	electron	1167:1174	arg1	microscopy					1176:1185	scanning electron microscopy	1158:1185	scanning electron microscopy	1158:1185	Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy.
31199973	2	71	theme	19.55 min	700:708	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	7	72	dep	improve	1540:1546	arg1	only					1527:1530	only	1527:1530	only	1527:1530	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	7	72	dep	improve	1540:1546	arg1	extract					1592:1598	extract	1592:1598	extract	1592:1598	Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.
31199973	2	73	theme	ratio	731:735	arg1	composition					561:571	the ATPS composition	552:571	the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50	552:740	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	2	74	theme	Key	420:422	arg1	factors					424:430	Key factors	420:430	Key factors in MAATPE process	420:448	Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50.
31199973	1	75	theme	diverse	271:277	arg1	polysaccharides					279:293	diverse polysaccharides	271:293	diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	271:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	6	76	theme	PTP	1430:1432	arg1	560 ± 4					1386:1392	560 ± 4	1386:1392	560 ± 4	1386:1392	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	6	76	theme	PTP	1430:1432	arg1	89.3 ± 2.4 μg/mL					1410:1425	89.3 ± 2.4 μg/mL	1410:1425	89.3 ± 2.4 μg/mL	1410:1425	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	6	76	theme	PTP	1430:1432	arg1	76.3 ± 2.2					1395:1404	76.3 ± 2.2	1395:1404	76.3 ± 2.2	1395:1404	Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively.
31199973	1	77	theme	Microwave-assisted	172:189	arg1	MAATPE					221:226	MAATPE	221:226	MAATPE	221:226	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	77	theme	Microwave-assisted	172:189	arg1	extraction					209:218	Microwave-assisted aqueous two-phase extraction	172:218	Microwave-assisted aqueous two-phase extraction (MAATPE)	172:227	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	4	78	from	structure	990:998	arg1	different					952:960	different	952:960	different	952:960	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	1	79	theme	polysaccharides	279:293	arg1	extraction					257:266	simultaneous extraction	244:266	simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	244:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	0	80	theme	Lentinus	80:87	arg1	edodes					89:94	Lentinus edodes	80:94	Lentinus edodes	80:94	Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
31199973	1	81	theme	sulfate	384:390	arg1	system					350:355	aqueous two-phase extraction system	321:355	aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant	321:417	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	81	theme	sulfate	384:390	arg1	ATPS					358:361	ATPS	358:361	ATPS	358:361	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	4	82	theme	chemical	981:988	arg1	structure					990:998	chemical structure	981:998	chemical structure	981:998	By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da.
31199973	1	83	theme	aqueous	191:197	arg1	MAATPE					221:226	MAATPE	221:226	MAATPE	221:226	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
31199973	1	83	theme	aqueous	191:197	arg1	extraction					209:218	Microwave-assisted aqueous two-phase extraction	172:218	Microwave-assisted aqueous two-phase extraction (MAATPE)	172:227	Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant.
30267785	0	0	theme	Extended	85:92	arg1	Delivery					94:101	Simultaneous Extended Delivery	72:101	Simultaneous Extended Delivery	72:101	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	8	1	theme	dosing	1484:1489	arg1	necessity					1462:1470	the necessity	1458:1470	the necessity of frequent dosing with a better disease management	1458:1522	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	6	2	theme	drug	1257:1260	arg1	bioavailability					1234:1248	the bioavailability	1230:1248	the bioavailability of the drug	1230:1260	The formula exhibited in vivo sustained release manner of MTG over 24 h and improved the bioavailability of the drug.
30267785	1	3	theme	in	174:175	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	3	theme	in	174:175	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	0	4	theme	Simultaneous	72:83	arg1	Delivery					94:101	Simultaneous Extended Delivery	72:101	Simultaneous Extended Delivery	72:101	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	8	5	theme	better	1498:1503	arg1	disease					1505:1511	a better disease	1496:1511	a better disease management	1496:1522	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	7	6	from	drugs	1333:1337	arg1	formulations					1367:1378	sustained-release liquid formulations	1342:1378	sustained-release liquid formulations	1342:1378	Thus, cosolvency presents a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations.
30267785	5	7	dep	%	921:921	arg1	carbonate					931:939	calcium carbonate	923:939	0.75% calcium carbonate	917:939	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	4	8	theme	in	587:588	arg1	formulations					599:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	3	9	dep	in	473:474	arg1	situ					476:479	situ	476:479	situ	476:479	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	5	10	dep	formulation	853:863	arg1	composition					866:876	composition	866:876	composition	866:876	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	4	11	theme	floating	763:770	arg1	ability					772:778	floating ability	763:778	floating ability	763:778	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	12	theme	gel	749:751	arg1	strength					753:760	gel strength	749:760	gel strength	749:760	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	6	13	theme	MTG	1203:1205	arg1	manner					1193:1198	in vivo sustained release manner	1167:1198	in vivo sustained release manner of MTG	1167:1205	The formula exhibited in vivo sustained release manner of MTG over 24 h and improved the bioavailability of the drug.
30267785	2	14	theme	mitiglinide	341:351	arg1	MTG					362:364	MTG	362:364	MTG	362:364	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	2	14	theme	mitiglinide	341:351	arg1	calcium					353:359	the novel antidiabetic drug mitiglinide calcium	313:359	the novel antidiabetic drug mitiglinide calcium (MTG)	313:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	5	15	theme	firm	1060:1063	arg1	gel					1065:1067	firm gel	1060:1067	firm gel that floated within seconds over the surface and remained buoyant for 24 h	1060:1142	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	6	16	theme	in vivo	1167:1173	arg1	release					1185:1191	in vivo sustained release	1167:1191	in vivo sustained release manner of MTG	1167:1205	The formula exhibited in vivo sustained release manner of MTG over 24 h and improved the bioavailability of the drug.
30267785	4	17	theme	gel	595:597	arg1	formulations					599:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	8	18	theme	disease	1505:1511	arg1	management					1513:1522	a better disease management	1496:1522	a better disease management	1496:1522	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	5	19	theme	optimized	843:851	arg1	formulation					853:863	The optimized formulation	839:863	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol)	839:967	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	5	20	theme	7.5	946:948	arg1	%					949:949	%	949:949	%	949:949	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	3	21	theme	cosolvency	422:431	arg1	approach					433:440	cosolvency approach	422:440	cosolvency approach	422:440	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	0	22	theme	Enhanced	107:114	arg1	Bioavailability					116:130	Enhanced Bioavailability	107:130	Enhanced Bioavailability of Mitiglinide Calcium	107:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	4	23	theme	ability	772:778	arg1	evaluation					827:836	phramacokinetics evaluation	810:836	phramacokinetics evaluation	810:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	23	theme	ability	772:778	arg1	characterization					718:733	characterization	718:733	characterization of viscosity, gel strength, floating ability, and in vitro MTG release	718:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	6	24	theme	sustained	1175:1183	arg1	release					1185:1191	in vivo sustained release	1167:1191	in vivo sustained release manner of MTG	1167:1205	The formula exhibited in vivo sustained release manner of MTG over 24 h and improved the bioavailability of the drug.
30267785	0	25	theme	Cosolvent-Based	16:30	arg1	Approach					59:66	a Promising Approach	47:66	a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium	47:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	0	25	theme	Cosolvent-Based	16:30	arg1	Gel					40:42	Cosolvent-Based In Situ Gel	16:42	Cosolvent-Based In Situ Gel	16:42	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	1	26	dep	in	174:175	arg1	situ					177:180	situ	177:180	situ	177:180	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	27	theme	novel	191:195	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	27	theme	novel	191:195	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	2	28	theme	drug	336:339	arg1	MTG					362:364	MTG	362:364	MTG	362:364	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	2	28	theme	drug	336:339	arg1	calcium					353:359	the novel antidiabetic drug mitiglinide calcium	313:359	the novel antidiabetic drug mitiglinide calcium (MTG)	313:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	7	29	theme	hydrophobic	1321:1331	arg1	drugs					1333:1337	hydrophobic drugs	1321:1337	hydrophobic drugs in sustained-release liquid formulations	1321:1378	Thus, cosolvency presents a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations.
30267785	1	30	theme	liquid	197:202	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	30	theme	liquid	197:202	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	8	31	theme	frequent	1475:1482	arg1	dosing					1484:1489	frequent dosing	1475:1489	frequent dosing with a better disease management	1475:1522	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	4	32	theme	viscosity	738:746	arg1	evaluation					827:836	phramacokinetics evaluation	810:836	phramacokinetics evaluation	810:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	32	theme	viscosity	738:746	arg1	characterization					718:733	characterization	718:733	characterization of viscosity, gel strength, floating ability, and in vitro MTG release	718:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	0	33	theme	Mitiglinide	135:145	arg1	Calcium					147:153	Mitiglinide Calcium	135:153	Mitiglinide Calcium	135:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	2	34	theme	antidiabetic	323:334	arg1	MTG					362:364	MTG	362:364	MTG	362:364	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	2	34	theme	antidiabetic	323:334	arg1	calcium					353:359	the novel antidiabetic drug mitiglinide calcium	313:359	the novel antidiabetic drug mitiglinide calcium (MTG)	313:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	5	35	theme	reasonable	979:988	arg1	viscosity					990:998	reasonable viscosity	979:998	reasonable viscosity	979:998	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	1	36	theme	sustained-release	204:220	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	36	theme	sustained-release	204:220	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	0	37	theme	In	32:33	arg1	Approach					59:66	a Promising Approach	47:66	a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium	47:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	0	37	theme	In	32:33	arg1	Gel					40:42	Cosolvent-Based In Situ Gel	16:42	Cosolvent-Based In Situ Gel	16:42	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	4	38	theme	MTG	583:585	arg1	formulations					599:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations	583:610	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	5	39	dep	%	898:898	arg1	alginate					907:914	sodium alginate	900:914	0.75% sodium alginate	894:914	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	2	40	theme	novel	317:321	arg1	MTG					362:364	MTG	362:364	MTG	362:364	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	2	40	theme	novel	317:321	arg1	calcium					353:359	the novel antidiabetic drug mitiglinide calcium	313:359	the novel antidiabetic drug mitiglinide calcium (MTG)	313:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	1	41	theme	drug	222:225	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	41	theme	drug	222:225	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	5	42	theme	sodium	900:905	arg1	alginate					907:914	sodium alginate	900:914	0.75% sodium alginate	894:914	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	3	43	theme	gastroretentive	457:471	arg1	gel					481:483	gastroretentive in situ gel	457:483	gastroretentive in situ gel of the short half-life	457:506	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	4	44	theme	phramacokinetics	810:825	arg1	evaluation					827:836	phramacokinetics evaluation	810:836	phramacokinetics evaluation	810:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	5	45	theme	%	880:880	arg1	gum					889:891	1% gellan gum	879:891	1% gellan gum	879:891	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	1	46	theme	delivery	227:234	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	46	theme	delivery	227:234	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	4	47	theme	polymer	688:694	arg1	solution					696:703	the polymer solution	684:703	the polymer solution	684:703	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	0	48	theme	Calcium	147:153	arg1	Delivery					94:101	Simultaneous Extended Delivery	72:101	Simultaneous Extended Delivery	72:101	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	0	48	theme	Calcium	147:153	arg1	Bioavailability					116:130	Enhanced Bioavailability	107:130	Enhanced Bioavailability of Mitiglinide Calcium	107:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	7	49	theme	liquid	1360:1365	arg1	formulations					1367:1378	sustained-release liquid formulations	1342:1378	sustained-release liquid formulations	1342:1378	Thus, cosolvency presents a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations.
30267785	3	50	theme	in	473:474	arg1	gel					481:483	gastroretentive in situ gel	457:483	gastroretentive in situ gel of the short half-life	457:506	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	0	51	theme	Promising	49:57	arg1	Approach					59:66	a Promising Approach	47:66	a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium	47:153	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	0	51	theme	Promising	49:57	arg1	Gel					40:42	Cosolvent-Based In Situ Gel	16:42	Cosolvent-Based In Situ Gel	16:42	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	5	52	theme	gellan	882:887	arg1	gum					889:891	1% gellan gum	879:891	1% gellan gum	879:891	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	4	53	dep	in	587:588	arg1	situ					590:593	situ	590:593	situ	590:593	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	5	54	dep	composition	866:876	arg1	glycol					961:966	7.5% propylene glycol	946:966	7.5% propylene glycol	946:966	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	5	54	dep	composition	866:876	arg1	%					921:921	0.75%	917:921	0.75% calcium carbonate	917:939	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	5	54	dep	composition	866:876	arg1	%					898:898	0.75%	894:898	0.75% sodium alginate	894:914	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	5	54	dep	composition	866:876	arg1	gum					889:891	1% gellan gum	879:891	1% gellan gum	879:891	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	3	55	theme	short	492:496	arg1	half-life					498:506	the short half-life	488:506	the short half-life	488:506	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	5	56	theme	calcium	923:929	arg1	carbonate					931:939	calcium carbonate	923:939	0.75% calcium carbonate	917:939	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	6	57	theme	release	1185:1191	arg1	manner					1193:1198	in vivo sustained release manner	1167:1198	in vivo sustained release manner of MTG	1167:1205	The formula exhibited in vivo sustained release manner of MTG over 24 h and improved the bioavailability of the drug.
30267785	3	58	theme	half-life	498:506	arg1	gel					481:483	gastroretentive in situ gel	457:483	gastroretentive in situ gel of the short half-life	457:506	Thus, our goal was to assess the possibility of using cosolvency approach in formulating gastroretentive in situ gel of the short half-life MTG to simultaneously enhance its bioavailability and sustain its release.
30267785	8	59	theme	diabetic	1413:1420	arg1	patients					1422:1429	diabetic patients'	1413:1430	diabetic patients' compliance	1413:1441	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	4	60	theme	in vitro	785:792	arg1	release					798:804	in vitro MTG release	785:804	in vitro MTG release	785:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	2	61	theme	water-soluble	285:297	arg1	drugs					299:303	poorly water-soluble drugs	278:303	poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG)	278:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	2	61	theme	water-soluble	285:297	arg1	calcium					353:359	the novel antidiabetic drug mitiglinide calcium	313:359	the novel antidiabetic drug mitiglinide calcium (MTG)	313:365	These systems are unsuitable for poorly water-soluble drugs such as the novel antidiabetic drug mitiglinide calcium (MTG).
30267785	4	62	theme	strength	753:760	arg1	evaluation					827:836	phramacokinetics evaluation	810:836	phramacokinetics evaluation	810:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	62	theme	strength	753:760	arg1	characterization					718:733	characterization	718:733	characterization of viscosity, gel strength, floating ability, and in vitro MTG release	718:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	5	63	theme	%	949:949	arg1	glycol					961:966	7.5% propylene glycol	946:966	7.5% propylene glycol	946:966	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	5	64	theme	simulated	1025:1033	arg1	fluid					1043:1047	simulated gastric fluid	1025:1047	simulated gastric fluid	1025:1047	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	0	65	dep	In	32:33	arg1	Situ					35:38	Situ	35:38	Situ	35:38	Gastroretentive Cosolvent-Based In Situ Gel as a Promising Approach for Simultaneous Extended Delivery and Enhanced Bioavailability of Mitiglinide Calcium.
30267785	7	66	theme	sustained-release	1342:1358	arg1	formulations					1367:1378	sustained-release liquid formulations	1342:1378	sustained-release liquid formulations	1342:1378	Thus, cosolvency presents a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations.
30267785	8	67	with	dosing	1484:1489	arg1	management					1513:1522	a better disease management	1496:1522	a better disease management	1496:1522	These formulations will improve diabetic patients' compliance by eliminating the necessity of frequent dosing with a better disease management.
30267785	5	68	theme	gastric	1035:1041	arg1	fluid					1043:1047	simulated gastric fluid	1025:1047	simulated gastric fluid	1025:1047	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	4	69	theme	release	798:804	arg1	evaluation					827:836	phramacokinetics evaluation	810:836	phramacokinetics evaluation	810:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	69	theme	release	798:804	arg1	characterization					718:733	characterization	718:733	characterization of viscosity, gel strength, floating ability, and in vitro MTG release	718:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	1	70	theme	Ion	156:158	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	70	theme	Ion	156:158	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	7	71	theme	promising	1291:1299	arg1	approach					1301:1308	a promising approach	1289:1308	a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations	1289:1378	Thus, cosolvency presents a promising approach to deliver hydrophobic drugs in sustained-release liquid formulations.
30267785	4	72	theme	propylene	633:641	arg1	glycol					643:648	propylene glycol	633:648	propylene glycol	633:648	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	4	72	theme	propylene	633:641	arg1	cosolvent					655:663	a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation	653:836	a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation	653:836	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	5	73	theme	propylene	951:959	arg1	glycol					961:966	7.5% propylene glycol	946:966	7.5% propylene glycol	946:966	The optimized formulation (composition: 1% gellan gum, 0.75% sodium alginate, 0.75% calcium carbonate, and 7.5% propylene glycol) exhibited reasonable viscosity but on introduction into simulated gastric fluid, it formed firm gel that floated within seconds over the surface and remained buoyant for 24 h.
30267785	4	74	theme	MTG	794:796	arg1	release					798:804	in vitro MTG release	785:804	in vitro MTG release	785:804	MTG in situ gel formulations were developed using propylene glycol as a cosolvent to dissolve MTG in the polymer solution, followed by characterization of viscosity, gel strength, floating ability, and in vitro MTG release and phramacokinetics evaluation.
30267785	1	75	theme	cross-linking	160:172	arg1	gels					182:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels	156:185	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30267785	1	75	theme	cross-linking	160:172	arg1	systems					236:242	novel liquid sustained-release drug delivery systems	191:242	novel liquid sustained-release drug delivery systems	191:242	Ion cross-linking in situ gels are novel liquid sustained-release drug delivery systems.
30189280	0	0	theme	chitosan/fucoidan	87:103	arg1	conjugates					105:114	N,O-carboxymethyl chitosan/fucoidan conjugates	69:114	conjugates	105:114	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	1	1	theme	great	175:179	arg1	promise					181:187	great promise	175:187	great promise	175:187	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	4	2	theme	NOCC/FD	608:614	arg1	scaffolds					616:624	the NOCC/FD scaffolds	604:624	the NOCC/FD scaffolds	604:624	To enhance the mechanical properties and osteogenic activity, the NOCC/FD scaffolds were biomineralized for the growth of hydroxyapatite crystals.
30189280	7	3	with	mineralization	1107:1120	arg1	n-HAp					1127:1131	n-HAp	1127:1131	n-HAp	1127:1131	The compressive strength of the scaffolds was greatly enhanced after mineralization with n-HAp.
30189280	5	4	theme	comparative	691:701	arg1	assessment					703:712	A comparative assessment	689:712	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds	689:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	8	5	theme	osteoblast	1226:1235	arg1	cells					1237:1241	osteoblast cells	1226:1241	osteoblast cells	1226:1241	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	0	6	theme	conjugates	105:114	arg1	biomineralization					48:64	biomineralization	48:64	biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering	48:142	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	9	7	theme	excellent	1373:1381	arg1	scaffolds					1344:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	9	7	theme	excellent	1373:1381	arg1	scaffold					1397:1404	an excellent and versatile scaffold	1370:1404	an excellent and versatile scaffold for bone tissue engineering	1370:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	2	8	theme	O-carboxymethyl	280:294	arg1	NOCC					306:309	NOCC	306:309	NOCC	306:309	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	2	8	theme	O-carboxymethyl	280:294	arg1	chitosan					296:303	O-carboxymethyl chitosan	280:303	O-carboxymethyl chitosan (NOCC)	280:310	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	0	9	theme	tissue	125:130	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	5	10	dep	structures	721:730	arg1	the					717:719	the	717:719	the	717:719	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	3	11	with	scaffolds	486:494	arg1	macropores					516:525	interconnected macropores	501:525	interconnected macropores	501:525	The hydrogels were lyophilized and then three-dimensional scaffolds with interconnected macropores were obtained.
30189280	3	12	theme	three-dimensional	468:484	arg1	scaffolds					486:494	then three-dimensional scaffolds	463:494	then three-dimensional scaffolds with interconnected macropores	463:525	The hydrogels were lyophilized and then three-dimensional scaffolds with interconnected macropores were obtained.
30189280	9	13	theme	versatile	1387:1395	arg1	scaffolds					1344:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	9	13	theme	versatile	1387:1395	arg1	scaffold					1397:1404	an excellent and versatile scaffold	1370:1404	an excellent and versatile scaffold for bone tissue engineering	1370:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	0	14	theme	bone	120:123	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	6	15	theme	nanocrystallites	921:936	arg1	growth					896:901	the growth	892:901	the growth of hydroxyapatite nanocrystallites (n-HAp)	892:944	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	0	16	dep	conjugates	105:114	arg1	O-carboxymethyl					71:85	N,O-carboxymethyl chitosan/fucoidan conjugates	69:114	O-carboxymethyl	71:85	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	1	17	theme	clinical	193:200	arg1	efficacy					202:209	clinical efficacy	193:209	clinical efficacy	193:209	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	6	18	theme	hydroxyapatite	906:919	arg1	n-HAp					939:943	n-HAp	939:943	n-HAp	939:943	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	6	18	theme	hydroxyapatite	906:919	arg1	nanocrystallites					921:936	hydroxyapatite nanocrystallites	906:936	hydroxyapatite nanocrystallites (n-HAp)	906:944	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	6	19	theme	NOCC	1022:1025	arg1	scaffolds					1027:1035	NOCC scaffolds	1022:1035	NOCC scaffolds	1022:1035	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	2	20	theme	amidation	366:374	arg1	reaction					376:383	an amidation reaction	363:383	an amidation reaction to synthesize NOCC/FD composite hydrogels	363:425	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	5	21	theme	X-ray	841:845	arg1	diffraction					847:857	X-ray diffraction	841:857	X-ray diffraction	841:857	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	4	22	theme	osteogenic	583:592	arg1	activity					594:601	osteogenic activity	583:601	osteogenic activity	583:601	To enhance the mechanical properties and osteogenic activity, the NOCC/FD scaffolds were biomineralized for the growth of hydroxyapatite crystals.
30189280	6	23	theme	NOCC/FD	959:965	arg1	scaffolds					967:975	thus the NOCC/FD scaffolds	950:975	thus the NOCC/FD scaffolds	950:975	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	8	24	theme	original	1266:1273	arg1	scaffolds					1296:1304	the original and mineralized NOCC scaffolds	1262:1304	the original and mineralized NOCC scaffolds	1262:1304	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	8	25	theme	cells	1237:1241	arg1	proliferation					1175:1187	proliferation	1175:1187	proliferation	1175:1187	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	8	25	theme	cells	1237:1241	arg1	activity					1194:1201	ALP activity	1190:1201	ALP activity	1190:1201	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	8	25	theme	cells	1237:1241	arg1	mineralization					1208:1221	mineralization	1208:1221	mineralization	1208:1221	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	8	26	theme	n-HAp/NOCC/FD	1138:1150	arg1	scaffolds					1152:1160	The n-HAp/NOCC/FD scaffolds	1134:1160	The n-HAp/NOCC/FD scaffolds	1134:1160	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	7	27	theme	compressive	1042:1052	arg1	strength					1054:1061	The compressive strength	1038:1061	The compressive strength of the scaffolds	1038:1078	The compressive strength of the scaffolds was greatly enhanced after mineralization with n-HAp.
30189280	4	28	theme	hydroxyapatite	664:677	arg1	crystals					679:686	hydroxyapatite crystals	664:686	hydroxyapatite crystals	664:686	To enhance the mechanical properties and osteogenic activity, the NOCC/FD scaffolds were biomineralized for the growth of hydroxyapatite crystals.
30189280	0	29	theme	nanocomposite	15:27	arg1	Development					0:10	Development	0:10	Development of nanocomposite	0:27	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	9	30	theme	tissue	1415:1420	arg1	engineering					1422:1432	bone tissue engineering	1410:1432	bone tissue engineering	1410:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	2	31	theme	composite	407:415	arg1	hydrogels					417:425	NOCC/FD composite hydrogels	399:425	NOCC/FD composite hydrogels	399:425	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	8	32	theme	mineralized	1279:1289	arg1	scaffolds					1296:1304	the original and mineralized NOCC scaffolds	1262:1304	the original and mineralized NOCC scaffolds	1262:1304	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	6	33	theme	mineralization	991:1004	arg1	efficiency					1006:1015	better mineralization efficiency	984:1015	better mineralization efficiency	984:1015	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	5	34	theme	physical	751:758	arg1	properties					760:769	physical properties	751:769	physical properties	751:769	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	2	35	theme	NOCC/FD	399:405	arg1	hydrogels					417:425	NOCC/FD composite hydrogels	399:425	NOCC/FD composite hydrogels	399:425	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	6	36	theme	better	984:989	arg1	efficiency					1006:1015	better mineralization efficiency	984:1015	better mineralization efficiency	984:1015	FD regulated the growth of hydroxyapatite nanocrystallites (n-HAp) and thus the NOCC/FD scaffolds showed better mineralization efficiency than NOCC scaffolds.
30189280	5	37	theme	properties	760:769	arg1	assessment					703:712	A comparative assessment	689:712	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds	689:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	9	38	theme	bone	1410:1413	arg1	engineering					1422:1432	bone tissue engineering	1410:1432	bone tissue engineering	1410:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	1	39	theme	bone	235:238	arg1	defects					240:246	bone defects	235:246	bone defects	235:246	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	5	40	theme	scaffolds	803:811	arg1	morphologies					733:744	morphologies	733:744	morphologies	733:744	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	5	40	theme	scaffolds	803:811	arg1	structures					721:730	structures	721:730	structures	721:730	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	5	40	theme	scaffolds	803:811	arg1	properties					760:769	physical properties	751:769	physical properties	751:769	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	7	41	theme	scaffolds	1070:1078	arg1	strength					1054:1061	The compressive strength	1038:1061	The compressive strength of the scaffolds	1038:1078	The compressive strength of the scaffolds was greatly enhanced after mineralization with n-HAp.
30189280	3	42	theme	interconnected	501:514	arg1	macropores					516:525	interconnected macropores	501:525	interconnected macropores	501:525	The hydrogels were lyophilized and then three-dimensional scaffolds with interconnected macropores were obtained.
30189280	8	43	theme	NOCC	1291:1294	arg1	scaffolds					1296:1304	the original and mineralized NOCC scaffolds	1262:1304	the original and mineralized NOCC scaffolds	1262:1304	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	5	44	theme	structures	721:730	arg1	assessment					703:712	A comparative assessment	689:712	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds	689:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	9	45	theme	n-HAp-mineralized	1318:1334	arg1	scaffolds					1344:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	9	45	theme	n-HAp-mineralized	1318:1334	arg1	scaffold					1397:1404	an excellent and versatile scaffold	1370:1404	an excellent and versatile scaffold for bone tissue engineering	1370:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	9	46	theme	NOCC/FD	1336:1342	arg1	scaffolds					1344:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	the n-HAp-mineralized NOCC/FD scaffolds	1314:1352	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	9	46	theme	NOCC/FD	1336:1342	arg1	scaffold					1397:1404	an excellent and versatile scaffold	1370:1404	an excellent and versatile scaffold for bone tissue engineering	1370:1432	Hence, the n-HAp-mineralized NOCC/FD scaffolds may prove to be an excellent and versatile scaffold for bone tissue engineering.
30189280	4	47	theme	mechanical	557:566	arg1	properties					568:577	the mechanical properties	553:577	the mechanical properties	553:577	To enhance the mechanical properties and osteogenic activity, the NOCC/FD scaffolds were biomineralized for the growth of hydroxyapatite crystals.
30189280	8	48	theme	ALP	1190:1192	arg1	activity					1194:1201	ALP activity	1190:1201	ALP activity	1190:1201	The n-HAp/NOCC/FD scaffolds enhanced the proliferation, ALP activity, and mineralization of osteoblast cells more strongly than the original and mineralized NOCC scaffolds.
30189280	0	49	dep	scaffolds	29:37	arg1	based					39:43	based	39:43	scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering	29:142	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	2	50	theme	amphoteric	267:276	arg1	N					278:278	an amphoteric N	264:278	an amphoteric N	264:278	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	1	51	theme	Bone	145:148	arg1	engineering					157:167	Bone tissue engineering	145:167	Bone tissue engineering	145:167	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	1	52	theme	defects	240:246	arg1	regeneration					219:230	the regeneration	215:230	the regeneration of bone defects	215:246	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	5	53	theme	morphologies	733:744	arg1	assessment					703:712	A comparative assessment	689:712	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds	689:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	1	54	theme	tissue	150:155	arg1	engineering					157:167	Bone tissue engineering	145:167	Bone tissue engineering	145:167	Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects.
30189280	5	55	theme	mineralized	791:801	arg1	scaffolds					803:811	the original and mineralized scaffolds	774:811	scaffolds	803:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	4	56	theme	crystals	679:686	arg1	growth					654:659	the growth	650:659	the growth of hydroxyapatite crystals	650:686	To enhance the mechanical properties and osteogenic activity, the NOCC/FD scaffolds were biomineralized for the growth of hydroxyapatite crystals.
30189280	5	57	theme	FT-IR	863:867	arg1	analysis					869:876	FT-IR analysis	863:876	FT-IR analysis	863:876	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
30189280	0	58	theme	N	69:69	arg1	conjugates					105:114	N,O-carboxymethyl chitosan/fucoidan conjugates	69:114	conjugates	105:114	Development of nanocomposite scaffolds based on biomineralization of N,O-carboxymethyl chitosan/fucoidan conjugates for bone tissue engineering.
30189280	2	59	attach	cross-linked	346:357	arg3	reaction					376:383	an amidation reaction	363:383	an amidation reaction to synthesize NOCC/FD composite hydrogels	363:425	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	2	59	attach	cross-linked	346:357	arg3	study					257:261	this study	252:261	this study	252:261	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	2	60	dep	reaction	376:383	arg1	synthesize					388:397	synthesize	388:397	to synthesize NOCC/FD composite hydrogels	385:425	In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels.
30189280	5	61	theme	original	778:785	arg1	scaffolds					803:811	the original and mineralized scaffolds	774:811	scaffolds	803:811	A comparative assessment of the structures, morphologies, and physical properties of the original and mineralized scaffolds were performed by SEM, EDS, X-ray diffraction and FT-IR analysis.
31525414	0	0	theme	haloarchaeon	101:112	arg1	mucosum					124:130	haloarchaeon Haloferax mucosum	101:130	haloarchaeon Haloferax mucosum	101:130	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	4	1	theme	EPS	712:714	arg1	dispersions					724:734	EPS aqueous dispersions	712:734	EPS aqueous dispersions	712:734	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	7	2	theme	hexane-in-water	1241:1255	arg1	emulsion					1257:1264	hexane-in-water emulsion	1241:1264	hexane-in-water emulsion	1241:1264	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	6	3	theme	wide	1080:1083	arg1	range					1085:1089	a wide range	1078:1089	a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M)	1078:1195	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	2	4	theme	protein	385:391	arg1	content					393:399	important protein content	375:399	important protein content of 10%	375:406	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	6	5	theme	emulsifying	1019:1029	arg1	activity					1031:1038	its emulsifying activity	1015:1038	its emulsifying activity	1015:1038	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	7	6	theme	viscoelastic	1202:1213	arg1	behavior					1215:1222	viscoelastic behavior	1202:1222	viscoelastic behavior	1202:1222	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	3	7	theme	culture	419:425	arg1	media					427:431	Different culture media	409:431	Different culture media compositions	409:444	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	7	8	theme	shear	1300:1304	arg1	measurements					1306:1317	oscillatory shear measurements	1288:1317	oscillatory shear measurements	1288:1317	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	3	9	theme	media	427:431	arg1	compositions					433:444	Different culture media compositions	409:444	Different culture media compositions	409:444	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	0	10	theme	Haloferax	114:122	arg1	mucosum					124:130	haloarchaeon Haloferax mucosum	101:130	haloarchaeon Haloferax mucosum	101:130	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	5	11	with	emulsions	888:896	arg1	oil					997:999	mineral oil	989:999	mineral oil	989:999	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	11	with	emulsions	888:896	arg1	solvents					922:929	different nonpolar solvents	903:929	different nonpolar solvents	903:929	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	11	with	emulsions	888:896	arg1	chloroform					962:971	chloroform	962:971	chloroform	962:971	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	11	with	emulsions	888:896	arg1	oil					981:983	castor oil	974:983	castor oil	974:983	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	11	with	emulsions	888:896	arg1	n-hexane					942:949	n-hexane	942:949	n-hexane	942:949	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	11	with	emulsions	888:896	arg1	kerosene					952:959	kerosene	952:959	kerosene	952:959	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	6	12	theme	concentrations	1163:1176	arg1	range					1085:1089	a wide range	1078:1089	a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M)	1078:1195	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	4	13	dep	96 h.	706:710	arg1	showed					736:741	showed	736:741	showed a non-Newtonian rheological behavior which was well fitted to the Cross equation	736:822	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	2	14	theme	important	375:383	arg1	content					393:399	important protein content	375:399	important protein content of 10%	375:406	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	6	15	theme	NaCl	1158:1161	arg1	concentrations					1163:1176	NaCl concentrations	1158:1176	NaCl concentrations (0, 2.0 and 4.0 M)	1158:1195	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	5	16	theme	castor	974:979	arg1	oil					981:983	castor oil	974:983	castor oil	974:983	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	1	17	theme	novel	213:217	arg1	EPS					238:240	EPS	238:240	EPS	238:240	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	1	17	theme	novel	213:217	arg1	exopolysaccharide					219:235	a novel exopolysaccharide	211:235	a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191)	211:295	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	5	18	theme	different	903:911	arg1	oil					997:999	mineral oil	989:999	mineral oil	989:999	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	18	theme	different	903:911	arg1	solvents					922:929	different nonpolar solvents	903:929	different nonpolar solvents	903:929	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	18	theme	different	903:911	arg1	chloroform					962:971	chloroform	962:971	chloroform	962:971	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	18	theme	different	903:911	arg1	oil					981:983	castor oil	974:983	castor oil	974:983	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	18	theme	different	903:911	arg1	n-hexane					942:949	n-hexane	942:949	n-hexane	942:949	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	18	theme	different	903:911	arg1	kerosene					952:959	kerosene	952:959	kerosene	952:959	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	1	19	dep	characterization	153:168	arg1	The					133:135	The	133:135	The	133:135	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	1	20	theme	exopolysaccharide	219:235	arg1	properties					197:206	emulsifying functional properties	174:206	emulsifying functional properties	174:206	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	1	20	theme	exopolysaccharide	219:235	arg1	characterization					153:168	physicochemical characterization	137:168	physicochemical characterization	137:168	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	3	21	theme	Different	409:417	arg1	media					427:431	Different culture media	409:431	Different culture media compositions	409:444	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	4	22	theme	rheological	759:769	arg1	behavior					771:778	a non-Newtonian rheological behavior	743:778	a non-Newtonian rheological behavior which was well fitted to the Cross equation	743:822	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	0	23	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	2	24	theme	molecular	343:351	arg1	weight					353:358	a high molecular weight	336:358	a high molecular weight of 152 kDa	336:369	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	2	25	theme	%	406:406	arg1	content					393:399	important protein content	375:399	important protein content of 10%	375:406	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	2	25	theme	%	406:406	arg1	weight					353:358	a high molecular weight	336:358	a high molecular weight of 152 kDa	336:369	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	0	26	theme	emulsifying	37:47	arg1	properties					49:58	emulsifying properties	37:58	emulsifying properties	37:58	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	2	27	theme	high	338:341	arg1	weight					353:358	a high molecular weight	336:358	a high molecular weight of 152 kDa	336:369	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	4	28	theme	highest	649:655	arg1	7.15 ± 0.44 g/L					673:687	7.15 ± 0.44 g/L	673:687	7.15 ± 0.44 g/L	673:687	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	4	28	theme	highest	649:655	arg1	production					661:670	The highest EPS production	645:670	The highest EPS production (7.15 ± 0.44 g/L)	645:688	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	4	29	theme	non-Newtonian	745:757	arg1	behavior					771:778	a non-Newtonian rheological behavior	743:778	a non-Newtonian rheological behavior which was well fitted to the Cross equation	743:822	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	3	30	theme	carbon	591:596	arg1	sources					598:604	carbon sources	591:604	carbon sources	591:604	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	3	31	from	EPS	621:623	arg1	medium					637:642	a liquid medium	628:642	a liquid medium	628:642	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	3	32	theme	yeast	551:555	arg1	extract					557:563	yeast extract	551:563	yeast extract	551:563	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	2	33	contain	has	332:334	arg1	biopolymer					321:330	This biopolymer	316:330	This biopolymer	316:330	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	2	33	contain	has	332:334	arg2	weight					353:358	a high molecular weight	336:358	a high molecular weight of 152 kDa	336:369	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	2	33	contain	has	332:334	arg2	content					393:399	important protein content	375:399	important protein content of 10%	375:406	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	5	34	theme	w/w	843:845	arg1	%					841:841	0.32% w/w	837:845	0.32% w/w	837:845	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	3	35	theme	ATCC	475:478	arg1	medium					485:490	the ATCC 2185 medium	471:490	the ATCC 2185 medium	471:490	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	3	35	theme	ATCC	475:478	arg1	base					497:500	a base	495:500	a base	495:500	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	1	36	theme	haloarchaea	255:265	arg1	DSM					286:288	DSM 27191	286:294	DSM 27191	286:294	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	1	36	theme	haloarchaea	255:265	arg1	mucosum					277:283	haloarchaea Haloferax mucosum	255:283	haloarchaea Haloferax mucosum (DSM 27191)	255:295	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	0	37	theme	novel	65:69	arg1	exopolysaccharide					71:87	a novel exopolysaccharide	63:87	a novel exopolysaccharide	63:87	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	5	38	theme	water-in-oil	875:886	arg1	emulsions					888:896	water-in-oil emulsions	875:896	water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil	875:999	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	7	39	theme	emulsion	1257:1264	arg1	stability					1228:1236	stability	1228:1236	stability	1228:1236	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	7	39	theme	emulsion	1257:1264	arg1	behavior					1215:1222	viscoelastic behavior	1202:1222	viscoelastic behavior	1202:1222	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	1	40	theme	physicochemical	137:151	arg1	characterization					153:168	physicochemical characterization	137:168	physicochemical characterization	137:168	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	6	41	dep	concentrations	1163:1176	arg1	4.0 M					1190:1194	4.0 M	1190:1194	4.0 M	1190:1194	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	6	41	dep	concentrations	1163:1176	arg1	2.0					1182:1184	2.0	1182:1184	2.0	1182:1184	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	7	42	dep	behavior	1215:1222	arg1	The					1198:1200	The	1198:1200	The	1198:1200	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	3	43	theme	varying	525:531	arg1	concentrations					533:546	varying concentrations	525:546	varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium	525:642	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	6	44	theme	pH	1132:1133	arg1	range					1085:1089	a wide range	1078:1089	a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M)	1078:1195	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	6	45	dep	temperatures	1094:1105	arg1	100 °C					1123:1128	100 °C	1123:1128	100 °C	1123:1128	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	6	45	dep	temperatures	1094:1105	arg1	70					1116:1117	70	1116:1117	70	1116:1117	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	4	46	theme	Cross	809:813	arg1	equation					815:822	the Cross equation	805:822	the Cross equation	805:822	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	5	47	from	%	841:841	arg1	EPS					829:831	The EPS	825:831	The EPS (at 0.32% w/w)	825:846	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	47	from	%	841:841	arg1	capable					852:858	capable	852:858	capable	852:858	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	2	48	theme	152 kDa	363:369	arg1	content					393:399	important protein content	375:399	important protein content of 10%	375:406	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	2	48	theme	152 kDa	363:369	arg1	weight					353:358	a high molecular weight	336:358	a high molecular weight of 152 kDa	336:369	This biopolymer has a high molecular weight of 152 kDa and important protein content of 10%.
31525414	7	49	theme	oscillatory	1288:1298	arg1	measurements					1306:1317	oscillatory shear measurements	1288:1317	oscillatory shear measurements	1288:1317	The viscoelastic behavior and stability of hexane-in-water emulsion were examined through oscillatory shear measurements.
31525414	1	50	theme	Haloferax	267:275	arg1	DSM					286:288	DSM 27191	286:294	DSM 27191	286:294	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	1	50	theme	Haloferax	267:275	arg1	mucosum					277:283	haloarchaea Haloferax mucosum	255:283	haloarchaea Haloferax mucosum (DSM 27191)	255:295	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	3	51	theme	glucose	569:575	arg1	concentrations					533:546	varying concentrations	525:546	varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium	525:642	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	5	52	theme	nonpolar	913:920	arg1	oil					997:999	mineral oil	989:999	mineral oil	989:999	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	52	theme	nonpolar	913:920	arg1	solvents					922:929	different nonpolar solvents	903:929	different nonpolar solvents	903:929	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	52	theme	nonpolar	913:920	arg1	chloroform					962:971	chloroform	962:971	chloroform	962:971	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	52	theme	nonpolar	913:920	arg1	oil					981:983	castor oil	974:983	castor oil	974:983	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	52	theme	nonpolar	913:920	arg1	n-hexane					942:949	n-hexane	942:949	n-hexane	942:949	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	52	theme	nonpolar	913:920	arg1	kerosene					952:959	kerosene	952:959	kerosene	952:959	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	5	53	theme	mineral	989:995	arg1	oil					997:999	mineral oil	989:999	mineral oil	989:999	The EPS (at 0.32% w/w) was capable of stabilizing water-in-oil emulsions with different nonpolar solvents, including n-hexane, kerosene, chloroform, castor oil and mineral oil.
31525414	1	54	theme	emulsifying	174:184	arg1	properties					197:206	emulsifying functional properties	174:206	emulsifying functional properties	174:206	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	6	55	theme	temperatures	1094:1105	arg1	range					1085:1089	a wide range	1078:1089	a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M)	1078:1195	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	4	56	theme	EPS	657:659	arg1	7.15 ± 0.44 g/L					673:687	7.15 ± 0.44 g/L	673:687	7.15 ± 0.44 g/L	673:687	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	4	56	theme	EPS	657:659	arg1	production					661:670	The highest EPS production	645:670	The highest EPS production (7.15 ± 0.44 g/L)	645:688	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
31525414	1	57	theme	functional	186:195	arg1	properties					197:206	emulsifying functional properties	174:206	emulsifying functional properties	174:206	The physicochemical characterization and emulsifying functional properties of a novel exopolysaccharide (EPS) produced by haloarchaea Haloferax mucosum (DSM 27191) were investigated.
31525414	3	58	theme	2185	480:483	arg1	medium					485:490	the ATCC 2185 medium	471:490	the ATCC 2185 medium	471:490	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	3	58	theme	2185	480:483	arg1	base					497:500	a base	495:500	a base	495:500	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	6	59	dep	pH	1132:1133	arg1	12					1150:1151	12	1150:1151	12	1150:1151	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	6	59	dep	pH	1132:1133	arg1	7					1144:1144	7	1144:1144	7	1144:1144	EPS retained its emulsifying activity after to be incubated for one hour in a wide range of temperatures (25, 40, 70 and 100 °C), pH (4, 6.5, 7 and 12) and NaCl concentrations (0, 2.0 and 4.0 M).
31525414	0	60	theme	exopolysaccharide	71:87	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	0	60	theme	exopolysaccharide	71:87	arg1	properties					49:58	emulsifying properties	37:58	emulsifying properties	37:58	Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.
31525414	3	61	theme	liquid	630:635	arg1	medium					637:642	a liquid medium	628:642	a liquid medium	628:642	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	3	62	theme	extract	557:563	arg1	concentrations					533:546	varying concentrations	525:546	varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium	525:642	Different culture media compositions were investigated taking the ATCC 2185 medium as a base and supplementing with varying concentrations of yeast extract and glucose or sucrose as carbon sources to produce the EPS in a liquid medium.
31525414	4	63	theme	aqueous	716:722	arg1	dispersions					724:734	EPS aqueous dispersions	712:734	EPS aqueous dispersions	712:734	The highest EPS production (7.15 ± 0.44 g/L) was obtained at 96 h. EPS aqueous dispersions showed a non-Newtonian rheological behavior which was well fitted to the Cross equation.
32293135	0	0	theme	Cancer	95:100	arg1	Cells					102:106	Drug-Surviving Cancer Cells	80:106	Drug-Surviving Cancer Cells	80:106	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	7	1	theme	drug	1556:1559	arg1	resistance					1561:1570	drug resistance	1556:1570	drug resistance	1556:1570	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	7	2	theme	drug-resistance	1445:1459	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	6	3	with	nanogels	1129:1136	arg1	lipids					1143:1148	lipids	1143:1148	lipids	1143:1148	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	2	4	theme	cytometry	484:492	arg1	analysis					494:501	flow cytometry analysis	479:501	flow cytometry analysis	479:501	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	2	5	theme	cell	353:356	arg1	subtypes					358:365	various cell subtypes	345:365	various cell subtypes from drug-surviving ovarian cancer cells	345:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	1	6	theme	great	261:265	arg1	significance					267:278	great significance	261:278	great significance	261:278	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	4	7	theme	migration	837:845	arg1	terms					822:826	terms	822:826	terms of their migration and invasion behavior	822:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	7	8	gly	heterogeneity	1358:1370	arg1	cells					1396:1400	drug-surviving tumor cells	1375:1400	drug-surviving tumor cells	1375:1400	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	7	8	gly	heterogeneity	1358:1370	arg1	differences					1430:1440	the significant differences	1414:1440	the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes	1414:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	7	9	theme	drug-surviving	1375:1388	arg1	cells					1396:1400	drug-surviving tumor cells	1375:1400	drug-surviving tumor cells	1375:1400	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	5	10	theme	drug	1041:1044	arg1	resistance					1046:1055	their drug resistance	1035:1055	their drug resistance	1035:1055	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	5	11	located	detected	914:921	arg2	genes					904:908	the phenotype switch genes	883:908	the phenotype switch genes	883:908	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	5	11	located	detected	914:921	arg1	addition					873:880	addition	873:880	addition	873:880	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	4	12	dep	subtype	775:781	arg1	the					751:753	the	751:753	the	751:753	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	7	13	theme	subtypes	1512:1519	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	6	14	theme	lipid	1223:1227	arg1	modification					1229:1240	the lipid modification	1219:1240	the lipid modification	1219:1240	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	5	15	theme	different	964:972	arg1	subtypes					974:981	different subtypes	964:981	different subtypes	964:981	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	6	16	theme	drug	1290:1293	arg1	carrier					1295:1301	a chemotherapy drug carrier	1275:1301	a chemotherapy drug carrier	1275:1301	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	4	17	theme	drug-resistant	755:768	arg1	subtype					775:781	drug-resistant cell subtype	755:781	drug-resistant cell subtype	755:781	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	5	18	theme	phenotype	887:895	arg1	switch					897:902	phenotype switch	887:902	the phenotype switch genes	883:908	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	3	19	theme	drug-surviving	530:543	arg1	cells					553:557	the drug-surviving OVCAR-3 cells	526:557	the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention	526:603	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	1	20	theme	tumor	185:189	arg1	heterogeneity					191:203	tumor heterogeneity	185:203	tumor heterogeneity	185:203	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	0	21	from	Detection	44:52	arg1	Cells					102:106	Drug-Surviving Cancer Cells	80:106	Drug-Surviving Cancer Cells	80:106	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	6	22	theme	drug-resistant	1311:1324	arg1	cells					1326:1330	drug-resistant cells	1311:1330	drug-resistant cells	1311:1330	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	4	23	from	subtype	811:817	arg1	terms					822:826	terms	822:826	terms of their migration and invasion behavior	822:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	0	24	theme	Fluorescent	0:10	arg1	Polysaccharide					12:25	Fluorescent Polysaccharide	0:25	Fluorescent Polysaccharide	0:25	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	0	25	theme	Drug-Surviving	80:93	arg1	Cells					102:106	Drug-Surviving Cancer Cells	80:106	Drug-Surviving Cancer Cells	80:106	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	4	26	theme	non-drug-resistant	787:804	arg1	subtype					811:817	non-drug-resistant cell subtype	787:817	non-drug-resistant cell subtype	787:817	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	2	27	theme	cancer	395:400	arg1	cells					402:406	drug-surviving ovarian cancer cells	372:406	drug-surviving ovarian cancer cells	372:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	2	28	theme	nanogels	452:459	arg1	series					427:432	a series	425:432	a series of polysaccharide nanogels	425:459	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	7	29	theme	different	1502:1510	arg1	subtypes					1512:1519	different subtypes	1502:1519	different subtypes	1502:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	6	30	theme	carrier	1295:1301	arg1	effectiveness					1258:1270	the effectiveness	1254:1270	the effectiveness of a chemotherapy drug carrier against drug-resistant cells	1254:1330	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	3	31	theme	paclitaxel	581:590	arg1	intervention					592:603	paclitaxel intervention	581:603	paclitaxel intervention	581:603	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	7	32	theme	significant	1418:1428	arg1	differences					1430:1440	the significant differences	1414:1440	the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes	1414:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	2	33	theme	ovarian	387:393	arg1	cells					402:406	drug-surviving ovarian cancer cells	372:406	drug-surviving ovarian cancer cells	372:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	2	34	theme	polysaccharide	437:450	arg1	nanogels					452:459	polysaccharide nanogels	437:459	polysaccharide nanogels	437:459	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	7	35	theme	cells	1396:1400	arg1	heterogeneity					1358:1370	the heterogeneity	1354:1370	the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes	1354:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	1	36	theme	Tumor	109:113	arg1	metastasis					115:124	Tumor metastasis	109:124	Tumor metastasis	109:124	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	5	37	theme	genetic	1000:1006	arg1	differences					1008:1018	significant genetic differences	988:1018	significant genetic differences	988:1018	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	7	38	theme	invasion	1477:1484	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	3	39	theme	cell	685:688	arg1	subtypes					690:697	drug-resistant and non-drug-resistant cell subtypes	647:697	drug-resistant and non-drug-resistant cell subtypes	647:697	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	4	40	theme	cell	806:809	arg1	subtype					811:817	non-drug-resistant cell subtype	787:817	non-drug-resistant cell subtype	787:817	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	1	41	theme	drug	143:146	arg1	resistance					148:157	drug resistance	143:157	drug resistance	143:157	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	2	42	theme	flow	479:482	arg1	analysis					494:501	flow cytometry analysis	479:501	flow cytometry analysis	479:501	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	3	43	theme	various	614:620	arg1	subtypes					690:697	drug-resistant and non-drug-resistant cell subtypes	647:697	drug-resistant and non-drug-resistant cell subtypes	647:697	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	3	43	theme	various	614:620	arg1	subtypes					627:634	various cell subtypes	614:634	various cell subtypes	614:634	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	4	44	dep	migration	837:845	arg1	behavior					860:867	behavior	860:867	behavior	860:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	5	45	theme	switch	897:902	arg1	genes					904:908	the phenotype switch genes	883:908	the phenotype switch genes	883:908	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	4	46	theme	significant	719:729	arg1	differences					731:741	significant differences	719:741	significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior	719:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	7	47	theme	migration	1462:1470	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	3	48	theme	cell	622:625	arg1	subtypes					690:697	drug-resistant and non-drug-resistant cell subtypes	647:697	drug-resistant and non-drug-resistant cell subtypes	647:697	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	3	48	theme	cell	622:625	arg1	subtypes					627:634	various cell subtypes	614:634	various cell subtypes	614:634	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	6	49	theme	polysaccharide	1114:1127	arg1	nanogels					1129:1136	modifying polysaccharide nanogels	1104:1136	modifying polysaccharide nanogels with lipids	1104:1148	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	0	50	theme	Tumor	57:61	arg1	Heterogeneity					63:75	Tumor Heterogeneity	57:75	Tumor Heterogeneity	57:75	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	3	51	theme	non-drug-resistant	666:683	arg1	subtypes					690:697	drug-resistant and non-drug-resistant cell subtypes	647:697	drug-resistant and non-drug-resistant cell subtypes	647:697	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	7	52	theme	differences	1430:1440	arg1	heterogeneity					1358:1370	the heterogeneity	1354:1370	the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes	1354:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	7	53	from	heterogeneity	1358:1370	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	2	54	theme	various	345:351	arg1	subtypes					358:365	various cell subtypes	345:365	various cell subtypes from drug-surviving ovarian cancer cells	345:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	5	55	theme	significant	988:998	arg1	differences					1008:1018	significant genetic differences	988:1018	significant genetic differences	988:1018	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	6	56	theme	chemotherapy	1277:1288	arg1	carrier					1295:1301	a chemotherapy drug carrier	1275:1301	a chemotherapy drug carrier	1275:1301	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	6	57	theme	modifying	1104:1112	arg1	nanogels					1129:1136	modifying polysaccharide nanogels	1104:1136	modifying polysaccharide nanogels with lipids	1104:1148	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	6	58	theme	nanogels	1176:1183	arg1	uptake					1166:1171	the uptake	1162:1171	the uptake of nanogels by drug-resistant cells	1162:1207	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	4	59	from	subtype	775:781	arg1	terms					822:826	terms	822:826	terms of their migration and invasion behavior	822:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	4	60	theme	invasion	851:858	arg1	terms					822:826	terms	822:826	terms of their migration and invasion behavior	822:867	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	2	61	from	cells	402:406	arg1	subtypes					358:365	various cell subtypes	345:365	various cell subtypes from drug-surviving ovarian cancer cells	345:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	5	62	theme	mRNA	926:929	arg1	sequencing					931:940	mRNA sequencing	926:940	mRNA sequencing	926:940	In addition, the phenotype switch genes are detected by mRNA sequencing, and it is found that different subtypes show significant genetic differences with regard to their drug resistance, metastasis, and proliferation.
32293135	3	63	theme	OVCAR-3	545:551	arg1	cells					553:557	the drug-surviving OVCAR-3 cells	526:557	the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention	526:603	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	7	64	from	differences	1430:1440	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	3	65	theme	drug-resistant	647:660	arg1	subtypes					690:697	drug-resistant and non-drug-resistant cell subtypes	647:697	drug-resistant and non-drug-resistant cell subtypes	647:697	The results show that the drug-surviving OVCAR-3 cells that are subjected to paclitaxel intervention comprise various cell subtypes, including drug-resistant and non-drug-resistant cell subtypes.
32293135	4	66	theme	cell	770:773	arg1	subtype					775:781	drug-resistant cell subtype	755:781	drug-resistant cell subtype	755:781	Besides, there are significant differences between the drug-resistant cell subtype and non-drug-resistant cell subtype in terms of their migration and invasion behavior.
32293135	7	67	theme	tumor	1390:1394	arg1	cells					1396:1400	drug-surviving tumor cells	1375:1400	drug-surviving tumor cells	1375:1400	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	7	68	from	cells	1396:1400	arg1	capabilities					1486:1497	drug-resistance, migration, and invasion capabilities	1445:1497	drug-resistance, migration, and invasion capabilities of different subtypes	1445:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	2	69	theme	drug-surviving	372:385	arg1	cells					402:406	drug-surviving ovarian cancer cells	372:406	drug-surviving ovarian cancer cells	372:406	The approach provides a way to identify and isolate various cell subtypes from drug-surviving ovarian cancer cells, by synthesizing a series of polysaccharide nanogels and using them in flow cytometry analysis.
32293135	1	70	theme	tumor	237:241	arg1	heterogeneity					243:255	tumor heterogeneity	237:255	tumor heterogeneity	237:255	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	0	71	theme	Heterogeneity	63:75	arg1	Detection					44:52	the Detection	40:52	the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells	40:106	Fluorescent Polysaccharide Nanogels for the Detection of Tumor Heterogeneity in Drug-Surviving Cancer Cells.
32293135	6	72	theme	drug-resistant	1188:1201	arg1	cells					1203:1207	drug-resistant cells	1188:1207	drug-resistant cells	1188:1207	In particular, modifying polysaccharide nanogels with lipids can promote the uptake of nanogels by drug-resistant cells, and thus the lipid modification can enhance the effectiveness of a chemotherapy drug carrier against drug-resistant cells.
32293135	1	73	contain	has	257:259	arg1	identification					219:232	the identification	215:232	the identification of tumor heterogeneity	215:255	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	1	73	contain	has	257:259	arg2	significance					267:278	great significance	261:278	great significance	261:278	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
32293135	7	74	from	capabilities	1486:1497	arg1	heterogeneity					1358:1370	the heterogeneity	1354:1370	the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes	1354:1519	These studies reveal the heterogeneity of drug-surviving tumor cells, as well as the significant differences in drug-resistance, migration, and invasion capabilities of different subtypes, and demonstrate a way to overcome drug resistance.
32293135	1	75	theme	heterogeneity	243:255	arg1	identification					219:232	the identification	215:232	the identification of tumor heterogeneity	215:255	Tumor metastasis, recurrence, and drug resistance have been associated with tumor heterogeneity, and thus the identification of tumor heterogeneity has great significance in medicine.
30007611	4	0	theme	layered	743:749	arg1	structures					751:760	the layered structures	739:760	the layered structures of KGM-MTM composite films	739:787	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	1	1	theme	brick	217:221	arg1	microstructures					235:249	'brick and mortar' microstructures	216:249	'brick and mortar' microstructures	216:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	3	2	dep	strength	627:634	arg1	116 MPa					637:643	116 MPa	637:643	Tensile strength: 116 MPa	619:643	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	1	3	with	films	205:209	arg1	microstructures					235:249	'brick and mortar' microstructures	216:249	'brick and mortar' microstructures	216:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	5	4	from	films	963:967	arg1	field					987:991	the biomedical field	972:991	the biomedical field	972:991	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	2	5	theme	designed	340:347	arg1	procedure					361:369	the designed fabrication procedure	336:369	the designed fabrication procedure	336:369	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	2	6	theme	fabrication	349:359	arg1	procedure					361:369	the designed fabrication procedure	336:369	the designed fabrication procedure	336:369	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	1	7	theme	building	316:323	arg1	nanosheets					302:311	KGM-MTM hybrid nanosheets	287:311	KGM-MTM hybrid nanosheets	287:311	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	1	7	theme	building	316:323	arg1	blocks					325:330	building blocks	316:330	building blocks	316:330	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	3	8	dep	performance	580:590	arg1	strength					627:634	Tensile strength	619:634	Tensile strength: 116 MPa	619:643	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	3	8	dep	performance	580:590	arg1	the					557:559	the	557:559	the	557:559	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	5	9	theme	antimicrobial	949:961	arg1	films					963:967	antimicrobial films	949:967	antimicrobial films in the biomedical field	949:991	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	5	9	theme	antimicrobial	949:961	arg1	them					918:921	them	918:921	them	918:921	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	3	10	theme	nacre-like	521:530	arg1	microstructures					532:546	The nacre-like microstructures	517:546	The nacre-like microstructures	517:546	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	5	11	theme	bacterial	888:896	arg1	growth					898:903	bacterial growth	888:903	bacterial growth	888:903	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	5	12	theme	biomedical	976:985	arg1	field					987:991	the biomedical field	972:991	the biomedical field	972:991	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	2	13	theme	composite	479:487	arg1	film					489:492	KGM-MTM composite film	471:492	KGM-MTM composite film	471:492	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	1	14	theme	mortar	227:232	arg1	microstructures					235:249	'brick and mortar' microstructures	216:249	'brick and mortar' microstructures	216:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	5	15	theme	KGM-MTM-Ag	838:847	arg1	films					859:863	KGM-MTM-Ag composite films	838:863	KGM-MTM-Ag composite films	838:863	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	1	16	theme	Artificial	128:137	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	2	17	theme	KGM	429:431	arg1	coating					433:439	KGM coating	429:439	KGM coating	429:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	0	18	theme	Silver	0:5	arg1	nanoparticles					7:19	Silver nanoparticles	0:19	Silver nanoparticles	0:19	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	4	19	theme	KGM-MTM	765:771	arg1	films					783:787	KGM-MTM composite films	765:787	KGM-MTM composite films	765:787	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	1	20	theme	nacre-like	139:148	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	0	21	theme	konjac	34:39	arg1	films					90:94	konjac glucomannan-montmorillonite nacre-like composite films	34:94	konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications	34:125	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	5	22	theme	composite	849:857	arg1	films					859:863	KGM-MTM-Ag composite films	838:863	KGM-MTM-Ag composite films	838:863	It was found that KGM-MTM-Ag composite films significantly suppress bacterial growth, which makes them potentially applicable as antimicrobial films in the biomedical field.
30007611	1	23	theme	konjac	150:155	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	4	24	theme	films	783:787	arg1	structures					751:760	the layered structures	739:760	the layered structures of KGM-MTM composite films	739:787	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	1	25	theme	glucomannan-Montmorillonite	157:183	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	4	26	theme	Ag	688:689	arg1	nanoparticles					691:703	Ag nanoparticles	688:703	Ag nanoparticles (Ag NPs)	688:712	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	4	26	theme	Ag	688:689	arg1	NPs					709:711	Ag NPs	706:711	Ag NPs	706:711	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	0	27	theme	nacre-like	69:78	arg1	films					90:94	konjac glucomannan-montmorillonite nacre-like composite films	34:94	konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications	34:125	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	2	28	theme	building	392:399	arg1	blocks					401:406	hybrid building blocks	385:406	hybrid building blocks with a thin layer of KGM coating	385:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	4	29	theme	Ag	706:707	arg1	nanoparticles					691:703	Ag nanoparticles	688:703	Ag nanoparticles (Ag NPs)	688:712	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	4	29	theme	Ag	706:707	arg1	NPs					709:711	Ag NPs	706:711	Ag NPs	706:711	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	3	30	theme	KGM-MTM	649:655	arg1	films					667:671	KGM-MTM composite films	649:671	KGM-MTM composite films	649:671	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	2	31	theme	MTM	448:450	arg1	nanosheets					452:461	the MTM nanosheets	444:461	the MTM nanosheets	444:461	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	0	32	theme	glucomannan-montmorillonite	41:67	arg1	films					90:94	konjac glucomannan-montmorillonite nacre-like composite films	34:94	konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications	34:125	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	2	33	with	blocks	401:406	arg1	layer					420:424	a thin layer	413:424	a thin layer of KGM coating	413:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	2	34	theme	KGM-MTM	471:477	arg1	film					489:492	KGM-MTM composite film	471:492	KGM-MTM composite film	471:492	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	4	35	theme	in	796:797	arg1	method					812:817	an in situ reduced method	793:817	an in situ reduced method	793:817	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	2	36	theme	vacuum	498:503	arg1	filtration					505:514	vacuum filtration	498:514	vacuum filtration	498:514	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	4	37	dep	in	796:797	arg1	situ					799:802	situ	799:802	situ	799:802	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	0	38	theme	composite	80:88	arg1	films					90:94	konjac glucomannan-montmorillonite nacre-like composite films	34:94	konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications	34:125	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	3	39	theme	light	561:565	arg1	performance					580:590	light transmission performance	561:590	light transmission performance	561:590	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	1	40	theme	KGM-MTM	186:192	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	3	41	theme	composite	657:665	arg1	films					667:671	KGM-MTM composite films	649:671	KGM-MTM composite films	649:671	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	2	42	theme	hybrid	385:390	arg1	blocks					401:406	hybrid building blocks	385:406	hybrid building blocks with a thin layer of KGM coating	385:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	3	43	theme	Tensile	619:625	arg1	strength					627:634	Tensile strength	619:634	Tensile strength: 116 MPa	619:643	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	3	44	theme	mechanical	596:605	arg1	properties					607:616	mechanical properties	596:616	mechanical properties	596:616	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	4	45	theme	reduced	804:810	arg1	method					812:817	an in situ reduced method	793:817	an in situ reduced method	793:817	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	1	46	theme	composite	195:203	arg1	films					205:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films	128:209	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures	128:249	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	4	47	theme	composite	773:781	arg1	films					783:787	KGM-MTM composite films	765:787	KGM-MTM composite films	765:787	Additionally, Ag nanoparticles (Ag NPs) can be incorporated into the layered structures of KGM-MTM composite films via an in situ reduced method.
30007611	0	48	theme	antibacterial	100:112	arg1	applications					114:125	antibacterial applications	100:125	antibacterial applications	100:125	Silver nanoparticles incorporated konjac glucomannan-montmorillonite nacre-like composite films for antibacterial applications.
30007611	3	49	theme	films	667:671	arg1	properties					607:616	mechanical properties	596:616	mechanical properties	596:616	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	3	49	theme	films	667:671	arg1	performance					580:590	light transmission performance	561:590	light transmission performance	561:590	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	3	50	theme	transmission	567:578	arg1	performance					580:590	light transmission performance	561:590	light transmission performance	561:590	The nacre-like microstructures enhanced the light transmission performance and mechanical properties (Tensile strength: 116 MPa) of KGM-MTM composite films.
30007611	2	51	theme	coating	433:439	arg1	layer					420:424	a thin layer	413:424	a thin layer of KGM coating	413:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	1	52	theme	KGM-MTM	287:293	arg1	nanosheets					302:311	KGM-MTM hybrid nanosheets	287:311	KGM-MTM hybrid nanosheets	287:311	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	1	52	theme	KGM-MTM	287:293	arg1	blocks					325:330	building blocks	316:330	building blocks	316:330	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	2	53	theme	thin	415:418	arg1	layer					420:424	a thin layer	413:424	a thin layer of KGM coating	413:439	In the designed fabrication procedure, we assembled hybrid building blocks with a thin layer of KGM coating on the MTM nanosheets to form KGM-MTM composite film via vacuum filtration.
30007611	1	54	theme	hybrid	295:300	arg1	nanosheets					302:311	KGM-MTM hybrid nanosheets	287:311	KGM-MTM hybrid nanosheets	287:311	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
30007611	1	54	theme	hybrid	295:300	arg1	blocks					325:330	building blocks	316:330	building blocks	316:330	Artificial nacre-like konjac glucomannan-Montmorillonite (KGM-MTM) composite films with 'brick and mortar' microstructures have been fabricated based on using KGM-MTM hybrid nanosheets as building blocks.
31899318	7	0	theme	41.3 ± 0.5	935:944	arg1	%					945:945	%	945:945	%	945:945	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	5	1	theme	content	666:672	arg1	influence					612:620	the influence	608:620	the influence of atomizing droplet size and initial solid content on encapsulation efficiency	608:700	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	7	2	theme	droplet	1002:1008	arg1	size					1010:1013	droplet size	1002:1013	droplet size	1002:1013	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	1	3	theme	novel	142:146	arg1	dispersions					164:174	novel amorphous solid dispersions	142:174	novel amorphous solid dispersions based on yeast glucan particles	142:206	In this work, novel amorphous solid dispersions based on yeast glucan particles were produced.
31899318	0	4	theme	spray	67:71	arg1	drying					73:78	spray drying	67:78	spray drying	67:78	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	7	5	theme	solid	984:988	arg1	content					990:996	initial solid content	976:996	initial solid content	976:996	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	1	6	theme	amorphous	148:156	arg1	dispersions					164:174	novel amorphous solid dispersions	142:174	novel amorphous solid dispersions based on yeast glucan particles	142:206	In this work, novel amorphous solid dispersions based on yeast glucan particles were produced.
31899318	7	7	theme	glucan	887:892	arg1	particles					894:902	glucan particles	887:902	glucan particles	887:902	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	4	8	dep	drugs	453:457	arg1	drugs					453:457	Model drugs	447:457	Model drugs ibuprofen and curcumin	447:480	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	4	8	dep	drugs	453:457	arg1	curcumin					473:480	curcumin	473:480	curcumin	473:480	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	4	8	dep	drugs	453:457	arg1	ibuprofen					459:467	ibuprofen	459:467	ibuprofen	459:467	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	8	9	theme	ibuprofen	1177:1185	arg1	encapsulation					1160:1172	complete encapsulation	1151:1172	complete encapsulation of ibuprofen and curcumin	1151:1198	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	0	10	from	Encapsulation	0:12	arg1	particles					54:62	yeast glucan particles	41:62	yeast glucan particles	41:62	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	5	11	theme	encapsulation	677:689	arg1	efficiency					691:700	encapsulation efficiency	677:700	encapsulation efficiency	677:700	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	8	12	theme	compound	1240:1247	arg1	precipitation					1212:1224	any precipitation	1208:1224	any precipitation of the active compound outside of the glucan particles	1208:1279	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	6	13	theme	droplet	774:780	arg1	sizes					782:786	larger droplet sizes	767:786	larger droplet sizes	767:786	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	5	14	theme	atomizing	625:633	arg1	size					643:646	atomizing droplet size	625:646	atomizing droplet size	625:646	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	3	15	with	amorphization	397:409	arg1	solubility					435:444	low water solubility	425:444	low water solubility	425:444	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	8	16	theme	curcumin	1191:1198	arg1	encapsulation					1160:1172	complete encapsulation	1151:1172	complete encapsulation of ibuprofen and curcumin	1151:1198	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	9	17	theme	amorphous	1329:1337	arg1	composites					1339:1348	completely amorphous composites	1318:1348	completely amorphous composites	1318:1348	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	5	18	theme	size	643:646	arg1	influence					612:620	the influence	608:620	the influence of atomizing droplet size and initial solid content on encapsulation efficiency	608:700	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	5	19	theme	droplet	635:641	arg1	size					643:646	atomizing droplet size	625:646	atomizing droplet size	625:646	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	0	20	dep	dispersion	89:98	arg1	properties					116:125	properties	116:125	properties	116:125	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	6	21	theme	solid	728:732	arg1	content					734:740	higher solid content	721:740	higher solid content	721:740	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	1	22	theme	solid	158:162	arg1	dispersions					164:174	novel amorphous solid dispersions	142:174	novel amorphous solid dispersions based on yeast glucan particles	142:206	In this work, novel amorphous solid dispersions based on yeast glucan particles were produced.
31899318	8	23	theme	drying	1052:1057	arg1	process					1059:1065	The spray drying process	1042:1065	The spray drying process	1042:1065	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	6	24	theme	encapsulation	803:815	arg1	efficiencies					817:828	higher encapsulation efficiencies	796:828	higher encapsulation efficiencies	796:828	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	6	25	theme	higher	721:726	arg1	content					734:740	higher solid content	721:740	higher solid content	721:740	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	9	26	theme	crude	1486:1490	arg1	drug					1492:1495	the micronized crude drug	1471:1495	the micronized crude drug	1471:1495	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	5	27	from	influence	612:620	arg1	efficiency					691:700	encapsulation efficiency	677:700	encapsulation efficiency	677:700	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	0	28	from	drugs	32:36	arg1	particles					54:62	yeast glucan particles	41:62	yeast glucan particles	41:62	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	5	29	theme	initial	652:658	arg1	content					666:672	initial solid content	652:672	initial solid content	652:672	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	6	30	theme	higher	796:801	arg1	efficiencies					817:828	higher encapsulation efficiencies	796:828	higher encapsulation efficiencies	796:828	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	9	31	theme	wettability	1367:1377	arg1	properties					1394:1403	outstanding wettability and dispersion properties	1355:1403	outstanding wettability and dispersion properties	1355:1403	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	9	32	theme	outstanding	1355:1365	arg1	properties					1394:1403	outstanding wettability and dispersion properties	1355:1403	outstanding wettability and dispersion properties	1355:1403	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	5	33	theme	solid	660:664	arg1	content					666:672	initial solid content	652:672	initial solid content	652:672	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	7	34	theme	ibuprofen	863:871	arg1	efficiency					849:858	The encapsulation efficiency	831:858	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles	831:902	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	3	35	theme	low	425:427	arg1	solubility					435:444	low water solubility	425:444	low water solubility	425:444	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	0	36	theme	soluble	24:30	arg1	drugs					32:36	poorly soluble drugs	17:36	poorly soluble drugs in yeast glucan particles	17:62	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	7	37	theme	encapsulation	835:847	arg1	efficiency					849:858	The encapsulation efficiency	831:858	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles	831:902	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	7	38	theme	64.3 ± 0.2	950:959	arg1	%					945:945	%	945:945	%	945:945	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	1	39	theme	yeast	185:189	arg1	particles					198:206	yeast glucan particles	185:206	yeast glucan particles	185:206	In this work, novel amorphous solid dispersions based on yeast glucan particles were produced.
31899318	3	40	theme	suitable	369:376	arg1	candidates					378:387	suitable candidates	369:387	suitable candidates for the amorphization of drugs with low water solubility	369:444	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	3	40	theme	suitable	369:376	arg1	particles					355:363	these particles	349:363	these particles	349:363	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	4	41	theme	Model	447:451	arg1	drugs					453:457	Model drugs	447:457	Model drugs ibuprofen and curcumin	447:480	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	4	41	theme	Model	447:451	arg1	curcumin					473:480	curcumin	473:480	curcumin	473:480	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	4	41	theme	Model	447:451	arg1	ibuprofen					459:467	ibuprofen	459:467	ibuprofen	459:467	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	7	42	theme	initial	976:982	arg1	content					990:996	initial solid content	976:996	initial solid content	976:996	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	1	43	theme	glucan	191:196	arg1	particles					198:206	yeast glucan particles	185:206	yeast glucan particles	185:206	In this work, novel amorphous solid dispersions based on yeast glucan particles were produced.
31899318	8	44	theme	active	1233:1238	arg1	compound					1240:1247	the active compound	1229:1247	the active compound outside of the glucan particles	1229:1279	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	9	45	theme	dispersion	1383:1392	arg1	properties					1394:1403	outstanding wettability and dispersion properties	1355:1403	outstanding wettability and dispersion properties	1355:1403	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	2	46	theme	glucan	229:234	arg1	particles					236:244	Yeast glucan particles	223:244	Yeast glucan particles	223:244	Yeast glucan particles are hollow and porous, and they are mainly composed of amorphous polysaccharides.
31899318	8	47	theme	glucan	1264:1269	arg1	particles					1271:1279	the glucan particles	1260:1279	the glucan particles	1260:1279	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	0	48	theme	drugs	32:36	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying	0:78	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	4	49	theme	spray	536:540	arg1	drying					542:547	spray drying	536:547	spray drying	536:547	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	3	50	theme	drugs	414:418	arg1	amorphization					397:409	the amorphization	393:409	the amorphization of drugs with low water solubility	393:444	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	8	51	theme	complete	1151:1158	arg1	encapsulation					1160:1172	complete encapsulation	1151:1172	complete encapsulation of ibuprofen and curcumin	1151:1198	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	2	52	theme	Yeast	223:227	arg1	particles					236:244	Yeast glucan particles	223:244	Yeast glucan particles	223:244	Yeast glucan particles are hollow and porous, and they are mainly composed of amorphous polysaccharides.
31899318	5	53	theme	Different	550:558	arg1	parameters					573:582	Different spray-drying parameters	550:582	Different spray-drying parameters	550:582	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	0	54	theme	glucan	47:52	arg1	particles					54:62	yeast glucan particles	41:62	yeast glucan particles	41:62	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	0	55	from	particles	54:62	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying	0:78	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	5	56	theme	spray-drying	560:571	arg1	parameters					573:582	Different spray-drying parameters	550:582	Different spray-drying parameters	550:582	Different spray-drying parameters were tested to evaluate the influence of atomizing droplet size and initial solid content on encapsulation efficiency.
31899318	0	57	theme	yeast	41:45	arg1	particles					54:62	yeast glucan particles	41:62	yeast glucan particles	41:62	Encapsulation of poorly soluble drugs in yeast glucan particles by spray drying improves dispersion and dissolution properties.
31899318	7	58	dep	%	945:945	arg1	to					947:948	to	947:948	to	947:948	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	8	59	theme	spray	1046:1050	arg1	process					1059:1065	The spray drying process	1042:1065	The spray drying process	1042:1065	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
31899318	9	60	theme	micronized	1475:1484	arg1	drug					1492:1495	the micronized crude drug	1471:1495	the micronized crude drug	1471:1495	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	6	61	theme	larger	767:772	arg1	sizes					782:786	larger droplet sizes	767:786	larger droplet sizes	767:786	It was shown that higher solid content and, more significantly, larger droplet sizes lead to higher encapsulation efficiencies.
31899318	3	62	theme	water	429:433	arg1	solubility					435:444	low water solubility	425:444	low water solubility	425:444	We hypothesized that these particles are suitable candidates for the amorphization of drugs with low water solubility.
31899318	7	63	theme	two-fluid	1024:1032	arg1	nozzle					1034:1039	the two-fluid nozzle	1020:1039	the two-fluid nozzle	1020:1039	The encapsulation efficiency of ibuprofen (10 wt%) into glucan particles was considerably improved from 41.3 ± 0.5% to 64.3 ± 0.2% by increasing initial solid content and droplet size with the two-fluid nozzle.
31899318	4	64	theme	glucan	516:521	arg1	particles					523:531	glucan particles	516:531	glucan particles	516:531	Model drugs ibuprofen and curcumin were successfully encapsulated in glucan particles by spray drying.
31899318	2	65	theme	amorphous	301:309	arg1	polysaccharides					311:325	amorphous polysaccharides	301:325	amorphous polysaccharides	301:325	Yeast glucan particles are hollow and porous, and they are mainly composed of amorphous polysaccharides.
31899318	9	66	theme	faster	1429:1434	arg1	rates					1448:1452	significantly faster dissolution rates	1415:1452	significantly faster dissolution rates	1415:1452	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	9	67	theme	dissolution	1436:1446	arg1	rates					1448:1452	significantly faster dissolution rates	1415:1452	significantly faster dissolution rates	1415:1452	Overall, it was possible to produce completely amorphous composites with outstanding wettability and dispersion properties, and with significantly faster dissolution rates when compared to the micronized crude drug.
31899318	8	68	theme	ultrasonic	1102:1111	arg1	nozzle					1113:1118	the ultrasonic nozzle	1098:1118	the ultrasonic nozzle	1098:1118	The spray drying process was further optimized by using the ultrasonic nozzle and it was possible to achieve complete encapsulation of ibuprofen and curcumin without any precipitation of the active compound outside of the glucan particles.
30241862	0	0	theme	type	101:104	arg1	diabetes					108:115	type 2 diabetes	101:115	type 2 diabetes in rats	101:123	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	6	1	theme	fatty	1028:1032	arg1	SCFAs					1041:1045	SCFAs	1041:1045	SCFAs	1041:1045	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	6	1	theme	fatty	1028:1032	arg1	acids					1034:1038	short-chain fatty acids	1016:1038	more colonic short-chain fatty acids (SCFAs)	1003:1046	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	6	2	theme	diabetic	979:986	arg1	rats					988:991	Meanwhile, FP-treated diabetic rats	957:991	Meanwhile, FP-treated diabetic rats	957:991	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	5	3	theme	diabetic	877:884	arg1	rats					886:889	diabetic rats	877:889	diabetic rats	877:889	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	6	4	theme	short-chain	1016:1026	arg1	SCFAs					1041:1045	SCFAs	1041:1045	SCFAs	1041:1045	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	6	4	theme	short-chain	1016:1026	arg1	acids					1034:1038	short-chain fatty acids	1016:1038	more colonic short-chain fatty acids (SCFAs)	1003:1046	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	3	5	theme	Momordica	582:590	arg1	polysaccharides					602:616	Momordica charantia polysaccharides	582:616	Momordica charantia polysaccharides	582:616	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	6	6	theme	NFP-treated	1081:1091	arg1	rats					1093:1096	NFP-treated rats	1081:1096	NFP-treated rats	1081:1096	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	6	7	theme	colonic	1008:1014	arg1	SCFAs					1041:1045	SCFAs	1041:1045	SCFAs	1041:1045	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	6	7	theme	colonic	1008:1014	arg1	acids					1034:1038	short-chain fatty acids	1016:1038	more colonic short-chain fatty acids (SCFAs)	1003:1046	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	3	8	theme	charantia	592:600	arg1	polysaccharides					602:616	Momordica charantia polysaccharides	582:616	Momordica charantia polysaccharides	582:616	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	4	9	from	hyperlipidemia	696:709	arg1	rats					745:748	diabetic rats	736:748	diabetic rats	736:748	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	4	10	theme	diabetic	736:743	arg1	rats					745:748	diabetic rats	736:748	diabetic rats	736:748	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	2	11	dep	charantia	423:431	arg1	NFP					441:443	NFP	441:443	NFP	441:443	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	2	11	dep	charantia	423:431	arg1	FP					434:435	FP	434:435	FP	434:435	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	5	12	dep	diversity	783:791	arg1	the					779:781	the	779:781	the	779:781	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	3	13	theme	molecular	547:555	arg1	weight					557:562	molecular weight	547:562	molecular weight	547:562	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	2	14	theme	diabetic	326:333	arg1	rats					335:338	streptozotocin-induced type 2 diabetic rats	296:338	streptozotocin-induced type 2 diabetic rats	296:338	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	4	15	from	hyperinsulinemia	678:693	arg1	rats					745:748	diabetic rats	736:748	diabetic rats	736:748	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	0	16	from	L.	50:51	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116	0:87	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	1	17	from	influence	130:138	arg1	structure					187:195	structure	187:195	structure	187:195	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	1	17	from	influence	130:138	arg1	effects					215:221	anti-diabetic effects	201:221	anti-diabetic effects	201:221	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	3	18	theme	polysaccharides	602:616	arg1	characterization					501:516	the physicochemical characterization	481:516	the physicochemical characterization	481:516	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	3	18	theme	polysaccharides	602:616	arg1	composition					534:544	monosaccharide composition	519:544	monosaccharide composition	519:544	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	3	18	theme	polysaccharides	602:616	arg1	weight					557:562	molecular weight	547:562	molecular weight	547:562	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	3	18	theme	polysaccharides	602:616	arg1	viscosity					569:577	viscosity	569:577	viscosity	569:577	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	7	19	theme	charantia	1198:1206	arg1	polysaccharides					1208:1222	Momordica charantia polysaccharides	1188:1222	Momordica charantia polysaccharides	1188:1222	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	2	20	from	charantia	423:431	arg1	polysaccharides					364:378	polysaccharides	364:378	polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP)	364:444	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	1	21	theme	anti-diabetic	201:213	arg1	effects					215:221	anti-diabetic effects	201:221	anti-diabetic effects	201:221	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	4	22	from	hyperglycemia	663:675	arg1	rats					745:748	diabetic rats	736:748	diabetic rats	736:748	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	7	23	theme	polysaccharides	1208:1222	arg1	effects					1177:1183	the anti-diabetes effects	1159:1183	the anti-diabetes effects of Momordica charantia polysaccharides in rats	1159:1230	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	3	24	theme	physicochemical	485:499	arg1	characterization					501:516	the physicochemical characterization	481:516	the physicochemical characterization	481:516	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	7	25	theme	gut	1290:1292	arg1	microbiota					1294:1303	gut microbiota	1290:1303	gut microbiota	1290:1303	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	4	26	with	Treatment	619:627	arg1	FP					634:635	FP	634:635	FP	634:635	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	6	27	theme	pH	1058:1059	arg1	values					1061:1066	lower pH values	1052:1066	lower pH values	1052:1066	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	5	28	theme	gut	810:812	arg1	microbiota					814:823	gut microbiota	810:823	gut microbiota (Lactococcus laudensis and Prevotella loescheii)	810:872	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	5	29	with	treatment	917:925	arg1	FP					932:933	FP	932:933	FP	932:933	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	1	30	theme	Momordica	226:234	arg1	polysaccharides					246:260	Momordica charantia polysaccharides	226:260	Momordica charantia polysaccharides	226:260	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	6	31	theme	lower	1052:1056	arg1	values					1061:1066	lower pH values	1052:1066	lower pH values	1052:1066	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	5	32	theme	microbiota	814:823	arg1	abundance					797:805	abundance	797:805	abundance	797:805	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	5	32	theme	microbiota	814:823	arg1	diversity					783:791	diversity	783:791	diversity	783:791	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	3	33	theme	monosaccharide	519:532	arg1	composition					534:544	monosaccharide composition	519:544	monosaccharide composition	519:544	Fermentation affected the physicochemical characterization, monosaccharide composition, molecular weight, and viscosity of Momordica charantia polysaccharides.
30241862	2	34	theme	High-fat	278:285	arg1	diet					287:290	High-fat diet	278:290	High-fat diet	278:290	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	1	35	theme	charantia	236:244	arg1	polysaccharides					246:260	Momordica charantia polysaccharides	226:260	Momordica charantia polysaccharides	226:260	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	6	36	dep	Meanwhile	957:965	arg1	FP-treated					968:977	FP-treated	968:977	FP-treated	968:977	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	7	37	from	effects	1177:1183	arg1	rats					1227:1230	rats	1227:1230	rats	1227:1230	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	6	38	theme	Meanwhile	957:965	arg1	rats					988:991	Meanwhile, FP-treated diabetic rats	957:991	Meanwhile, FP-treated diabetic rats	957:991	Meanwhile, FP-treated diabetic rats exhibited more colonic short-chain fatty acids (SCFAs) and lower pH values than that in NFP-treated rats.
30241862	1	39	theme	polysaccharides	246:260	arg1	structure					187:195	structure	187:195	structure	187:195	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	1	39	theme	polysaccharides	246:260	arg1	effects					215:221	anti-diabetic effects	201:221	anti-diabetic effects	201:221	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	0	40	theme	Lactobacillus	58:70	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	4	41	from	stress	726:731	arg1	rats					745:748	diabetic rats	736:748	diabetic rats	736:748	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	1	42	dep	structure	187:195	arg1	the					183:185	the	183:185	the	183:185	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	7	43	theme	Lactobacillus	1108:1120	arg1	plantarum-fermentation					1122:1143	Lactobacillus plantarum-fermentation	1108:1143	Lactobacillus plantarum-fermentation	1108:1143	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	2	44	theme	Momordica	413:421	arg1	charantia					423:431	fermented and non-fermented Momordica charantia	385:431	fermented and non-fermented Momordica charantia (FP and NFP)	385:444	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	7	45	theme	polysaccharides	1262:1276	arg1	structure					1249:1257	the structure	1245:1257	the structure of polysaccharides	1245:1276	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	7	46	theme	SCFAs	1336:1340	arg1	production					1322:1331	the production	1318:1331	the production of SCFAs	1318:1340	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	2	47	theme	non-fermented	399:411	arg1	charantia					423:431	fermented and non-fermented Momordica charantia	385:431	fermented and non-fermented Momordica charantia (FP and NFP)	385:444	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	0	48	with	Polysaccharide	0:13	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	4	49	theme	oxidative	716:724	arg1	stress					726:731	oxidative stress	716:731	oxidative stress	716:731	Treatment with FP significantly ameliorated hyperglycemia, hyperinsulinemia, hyperlipidemia, and oxidative stress in diabetic rats compared with NFP.
30241862	7	50	theme	Momordica	1188:1196	arg1	polysaccharides					1208:1222	Momordica charantia polysaccharides	1188:1222	Momordica charantia polysaccharides	1188:1222	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	0	51	from	diabetes	108:115	arg1	rats					120:123	rats	120:123	rats	120:123	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	2	52	theme	streptozotocin-induced	296:317	arg1	rats					335:338	streptozotocin-induced type 2 diabetic rats	296:338	streptozotocin-induced type 2 diabetic rats	296:338	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	2	53	theme	fermented	385:393	arg1	charantia					423:431	fermented and non-fermented Momordica charantia	385:431	fermented and non-fermented Momordica charantia (FP and NFP)	385:444	High-fat diet and streptozotocin-induced type 2 diabetic rats were administrated with polysaccharides from fermented and non-fermented Momordica charantia (FP and NFP) for 4 weeks.
30241862	5	54	from	abundance	797:805	arg1	rats					886:889	diabetic rats	877:889	diabetic rats	877:889	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	5	55	dep	microbiota	814:823	arg1	laudensis					838:846	Lactococcus laudensis	826:846	Lactococcus laudensis	826:846	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	5	55	dep	microbiota	814:823	arg1	loescheii					863:871	Prevotella loescheii	852:871	Prevotella loescheii	852:871	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30241862	1	56	theme	Lactobacillus	143:155	arg1	plantarum-fermentation					157:178	Lactobacillus plantarum-fermentation	143:178	Lactobacillus plantarum-fermentation	143:178	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	0	57	theme	plantarum	72:80	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats.
30241862	7	58	theme	anti-diabetes	1163:1175	arg1	effects					1177:1183	the anti-diabetes effects	1159:1183	the anti-diabetes effects of Momordica charantia polysaccharides in rats	1159:1230	Overall, Lactobacillus plantarum-fermentation could enhance the anti-diabetes effects of Momordica charantia polysaccharides in rats by modifying the structure of polysaccharides to optimize gut microbiota and heighten the production of SCFAs.
30241862	1	59	theme	plantarum-fermentation	157:178	arg1	influence					130:138	The influence	126:138	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides	126:260	The influence of Lactobacillus plantarum-fermentation on the structure and anti-diabetic effects of Momordica charantia polysaccharides were evaluated.
30241862	5	60	from	diversity	783:791	arg1	rats					886:889	diabetic rats	877:889	diabetic rats	877:889	Moreover, the diversity and abundance of gut microbiota (Lactococcus laudensis and Prevotella loescheii) in diabetic rats were notably increased by treatment with FP in comparison to NFP.
30448492	1	0	theme	phosphorylated	150:163	arg1	P-NC					180:183	P-NC	180:183	P-NC	180:183	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	1	0	theme	phosphorylated	150:163	arg1	nanocellulose					165:177	phosphorylated nanocellulose	150:177	phosphorylated nanocellulose (P-NC)	150:184	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	5	1	theme	scanning	753:760	arg1	microscopy					771:780	environmental scanning electron microscopy	739:780	environmental scanning electron microscopy	739:780	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	2	theme	energy-dispersive	783:799	arg1	spectroscopy					807:818	energy-dispersive X-ray spectroscopy	783:818	energy-dispersive X-ray spectroscopy	783:818	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	3	3	from	effects	482:488	arg1	composites					543:552	the resulting jute fabric composites	517:552	the resulting jute fabric composites	517:552	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	5	4	theme	CS	933:934	arg1	macromolecules					936:949	CS macromolecules	933:949	CS macromolecules	933:949	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	1	5	theme	solvent-free	238:249	arg1	procedure					267:275	a solvent-free acid hydrolysis procedure	236:275	a solvent-free acid hydrolysis procedure	236:275	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	0	6	theme	thermal	108:114	arg1	stability					116:124	thermal stability	108:124	thermal stability	108:124	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	2	7	theme	different	287:295	arg1	procedures					307:316	Eighteen different treatment procedures	278:316	Eighteen different treatment procedures	278:316	Eighteen different treatment procedures were employed for the composite treatment of jute fabric.
30448492	1	8	theme	acid	251:254	arg1	procedure					267:275	a solvent-free acid hydrolysis procedure	236:275	a solvent-free acid hydrolysis procedure	236:275	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	3	9	theme	jute	531:534	arg1	composites					543:552	the resulting jute fabric composites	517:552	the resulting jute fabric composites	517:552	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	1	10	theme	hydrolysis	256:265	arg1	procedure					267:275	a solvent-free acid hydrolysis procedure	236:275	a solvent-free acid hydrolysis procedure	236:275	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	5	11	theme	environmental	739:751	arg1	microscopy					771:780	environmental scanning electron microscopy	739:780	environmental scanning electron microscopy	739:780	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	3	12	theme	chitosan	439:446	arg1	fractions					386:394	Different fractions	376:394	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%)	376:455	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	4	13	theme	phosphorus	570:579	arg1	content					581:587	phosphorus content	570:587	phosphorus content	570:587	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	3	14	theme	phosphorylated	399:412	arg1	nanocellulose					414:426	phosphorylated nanocellulose	399:426	phosphorylated nanocellulose (1-4%)	399:433	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	3	14	theme	phosphorylated	399:412	arg1	%					432:432	1-4%	429:432	1-4%	429:432	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	0	15	theme	functional	5:14	arg1	fabric					21:26	Dual functional jute fabric	0:26	Dual functional jute fabric	0:26	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	3	16	theme	procedures	503:512	arg1	effects					482:488	the effects	478:488	the effects of treatment procedures on the resulting jute fabric composites	478:552	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	5	17	theme	interaction	900:910	arg1	mechanism					887:895	the mechanism	883:895	the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs	883:960	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	0	18	theme	Dual	0:3	arg1	fabric					21:26	Dual functional jute fabric	0:26	Dual functional jute fabric	0:26	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	6	19	theme	antimicrobial	995:1007	arg1	development					980:990	the development	976:990	the development of antimicrobial and thermal stability properties on jute fabric	976:1055	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	3	20	theme	Different	376:384	arg1	fractions					386:394	Different fractions	376:394	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%)	376:455	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	6	21	from	development	980:990	arg1	fabric					1050:1055	jute fabric	1045:1055	jute fabric	1045:1055	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	0	22	dep	nano-cellulose	74:87	arg1	antimicrobial					90:102	antimicrobial	90:102	antimicrobial	90:102	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	0	22	dep	nano-cellulose	74:87	arg1	stability					116:124	thermal stability	108:124	thermal stability	108:124	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	4	23	theme	treated	619:625	arg1	fabrics					632:638	the treated jute fabrics	615:638	the treated jute fabrics	615:638	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	0	24	theme	jute	16:19	arg1	fabric					21:26	Dual functional jute fabric	0:26	Dual functional jute fabric	0:26	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	4	25	theme	Weight	555:560	arg1	uptake					562:567	Weight uptake	555:567	Weight uptake	555:567	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	4	26	theme	tensile	593:599	arg1	properties					601:610	tensile properties	593:610	tensile properties	593:610	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	2	27	theme	treatment	297:305	arg1	procedures					307:316	Eighteen different treatment procedures	278:316	Eighteen different treatment procedures	278:316	Eighteen different treatment procedures were employed for the composite treatment of jute fabric.
30448492	5	28	theme	jute	920:923	arg1	fabric					925:930	jute fabric	920:930	jute fabric	920:930	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	29	theme	X-ray	801:805	arg1	spectroscopy					807:818	energy-dispersive X-ray spectroscopy	783:818	energy-dispersive X-ray spectroscopy	783:818	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	30	theme	transmission	705:716	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy	705:736	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	4	31	theme	fabrics	632:638	arg1	uptake					562:567	Weight uptake	555:567	Weight uptake	555:567	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	4	31	theme	fabrics	632:638	arg1	content					581:587	phosphorus content	570:587	phosphorus content	570:587	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	4	31	theme	fabrics	632:638	arg1	properties					601:610	tensile properties	593:610	tensile properties	593:610	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	3	32	theme	0.5-2	449:453	arg1	chitosan					439:446	chitosan	439:446	chitosan (0.5-2%)	439:455	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	3	32	theme	0.5-2	449:453	arg1	%					454:454	0.5-2%	449:454	0.5-2%	449:454	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	2	33	theme	jute	363:366	arg1	fabric					368:373	jute fabric	363:373	jute fabric	363:373	Eighteen different treatment procedures were employed for the composite treatment of jute fabric.
30448492	5	34	theme	electron	718:725	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy	705:736	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	6	35	theme	stability	1021:1029	arg1	development					980:990	the development	976:990	the development of antimicrobial and thermal stability properties on jute fabric	976:1055	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	3	36	used	used	462:465	arg2	fractions					386:394	Different fractions	376:394	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%)	376:455	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	1	37	theme	date	209:212	arg1	fibers					226:231	date palm sheath fibers	209:231	date palm sheath fibers	209:231	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	3	38	theme	nanocellulose	414:426	arg1	fractions					386:394	Different fractions	376:394	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%)	376:455	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	5	39	theme	ATR-FTIR	660:667	arg1	spectroscopy					669:680	ATR-FTIR spectroscopy	660:680	ATR-FTIR spectroscopy	660:680	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	40	theme	thermo-gravimetric	824:841	arg1	analysis					843:850	thermo-gravimetric analysis	824:850	thermo-gravimetric analysis	824:850	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy	705:736	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	diffractometer					689:702	X-ray diffractometer	683:702	X-ray diffractometer	683:702	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	spectroscopy					807:818	energy-dispersive X-ray spectroscopy	783:818	energy-dispersive X-ray spectroscopy	783:818	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	microscopy					771:780	environmental scanning electron microscopy	739:780	environmental scanning electron microscopy	739:780	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	analysis					843:850	thermo-gravimetric analysis	824:850	thermo-gravimetric analysis	824:850	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	5	41	used	used	857:860	arg2	spectroscopy					669:680	ATR-FTIR spectroscopy	660:680	ATR-FTIR spectroscopy	660:680	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	6	42	theme	thermal	1013:1019	arg1	stability					1021:1029	thermal stability	1013:1029	thermal stability	1013:1029	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	2	43	theme	composite	340:348	arg1	treatment					350:358	the composite treatment	336:358	the composite treatment of jute fabric	336:373	Eighteen different treatment procedures were employed for the composite treatment of jute fabric.
30448492	3	44	theme	fabric	536:541	arg1	composites					543:552	the resulting jute fabric composites	517:552	the resulting jute fabric composites	517:552	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	1	45	theme	palm	214:217	arg1	fibers					226:231	date palm sheath fibers	209:231	date palm sheath fibers	209:231	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	5	46	theme	X-ray	683:687	arg1	diffractometer					689:702	X-ray diffractometer	683:702	X-ray diffractometer	683:702	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	6	47	dep	antimicrobial	995:1007	arg1	properties					1031:1040	properties	1031:1040	properties	1031:1040	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	3	48	theme	resulting	521:529	arg1	composites					543:552	the resulting jute fabric composites	517:552	the resulting jute fabric composites	517:552	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	1	49	theme	present	135:141	arg1	study					143:147	the present study	131:147	the present study	131:147	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	1	50	theme	sheath	219:224	arg1	fibers					226:231	date palm sheath fibers	209:231	date palm sheath fibers	209:231	In the present study, phosphorylated nanocellulose (P-NC) has been prepared from date palm sheath fibers by a solvent-free acid hydrolysis procedure.
30448492	5	51	theme	electron	762:769	arg1	microscopy					771:780	environmental scanning electron microscopy	739:780	environmental scanning electron microscopy	739:780	ATR-FTIR spectroscopy, X-ray diffractometer, transmission electron microscopy, environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy and thermo-gravimetric analysis were used to gain insight into the mechanism of interaction between jute fabric, CS macromolecules, and P-NCs.
30448492	4	52	theme	jute	627:630	arg1	fabrics					632:638	the treated jute fabrics	615:638	the treated jute fabrics	615:638	Weight uptake, phosphorus content and tensile properties of the treated jute fabrics have been reported.
30448492	3	53	theme	treatment	493:501	arg1	procedures					503:512	treatment procedures	493:512	treatment procedures	493:512	Different fractions of phosphorylated nanocellulose (1-4%) and chitosan (0.5-2%) were used to examine the effects of treatment procedures on the resulting jute fabric composites.
30448492	6	54	theme	jute	1045:1048	arg1	fabric					1050:1055	jute fabric	1045:1055	jute fabric	1045:1055	In addition, the development of antimicrobial and thermal stability properties on jute fabric has been investigated.
30448492	0	55	theme	phosphorylated	59:72	arg1	nano-cellulose					74:87	phosphorylated nano-cellulose	59:87	phosphorylated nano-cellulose (antimicrobial and thermal stability)	59:125	Dual functional jute fabric biocomposite with chitosan and phosphorylated nano-cellulose (antimicrobial and thermal stability).
30448492	2	56	theme	fabric	368:373	arg1	treatment					350:358	the composite treatment	336:358	the composite treatment of jute fabric	336:373	Eighteen different treatment procedures were employed for the composite treatment of jute fabric.
30606569	2	0	theme	hydrogel	467:474	arg1	WU					447:448	the WU	443:448	the WU of the synthetic hydrogel	443:474	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	3	1	theme	surface	495:501	arg1	morphologies					503:514	The surface morphologies	491:514	The surface morphologies of the hydrogels	491:531	The surface morphologies of the hydrogels showed that the pectin and κ-carrageenan favors the formation of larger and more defined pores.
30606569	6	2	theme	in	1033:1034	arg1	assays					1055:1060	in vitro cytotoxicity assays	1033:1060	in vitro cytotoxicity assays	1033:1060	The biocompatibility of the hydrogels was evaluated with in vitro cytotoxicity assays and the results indicated that they could be considered as candidates for biomedical use.
30606569	4	3	theme	hydrogel	800:807	arg1	properties					772:781	the mechanical properties	757:781	the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load	757:845	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	5	4	theme	mechanical	953:962	arg1	properties					964:973	the mechanical properties	949:973	the mechanical properties	949:973	Therefore, the entrapment of κ-carrageenan within synthetic hydrogels improved both the swelling and the mechanical properties.
30606569	2	5	dep	κ-carrageenan	365:377	arg1	hydrogels					392:400	hydrogels	392:400	hydrogels	392:400	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	3	6	theme	hydrogels	523:531	arg1	morphologies					503:514	The surface morphologies	491:514	The surface morphologies of the hydrogels	491:531	The surface morphologies of the hydrogels showed that the pectin and κ-carrageenan favors the formation of larger and more defined pores.
30606569	5	7	theme	κ-carrageenan	877:889	arg1	entrapment					863:872	the entrapment	859:872	the entrapment of κ-carrageenan within synthetic hydrogels	859:916	Therefore, the entrapment of κ-carrageenan within synthetic hydrogels improved both the swelling and the mechanical properties.
30606569	2	8	theme	chitosan	383:390	arg1	uptake					350:355	the water uptake	340:355	the water uptake (WU) of κ-carrageenan and chitosan hydrogels	340:400	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	8	theme	chitosan	383:390	arg1	3.5					413:415	3.5	413:415	3.5	413:415	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	8	theme	chitosan	383:390	arg1	WU					358:359	WU	358:359	WU	358:359	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	1	9	theme	chitosan	186:193	arg1	properties					301:310	the mechanical properties	286:310	the mechanical properties	286:310	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	1	9	theme	chitosan	186:193	arg1	entrapment					172:181	the entrapment	168:181	the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling	168:280	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	1	10	theme	mechanical	290:299	arg1	properties					301:310	the mechanical properties	286:310	the mechanical properties	286:310	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	4	11	theme	mechanical	761:770	arg1	properties					772:781	the mechanical properties	757:781	the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load	757:845	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	4	12	theme	compression	830:840	arg1	load					842:845	compression load	830:845	compression load	830:845	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	0	13	theme	chitosan	14:21	arg1	Entrapment					0:9	Entrapment	0:9	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.	0:129	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	0	14	theme	mechanical	108:117	arg1	properties					119:128	mechanical properties	108:128	mechanical properties	108:128	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	1	15	theme	pectin	196:201	arg1	properties					301:310	the mechanical properties	286:310	the mechanical properties	286:310	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	1	15	theme	pectin	196:201	arg1	entrapment					172:181	the entrapment	168:181	the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling	168:280	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	2	16	theme	synthetic	457:465	arg1	hydrogel					467:474	the synthetic hydrogel	453:474	the synthetic hydrogel	453:474	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	17	dep	3.5	413:415	arg1	higher					431:436	higher	431:436	higher	431:436	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	5	18	theme	synthetic	898:906	arg1	hydrogels					908:916	synthetic hydrogels	898:916	synthetic hydrogels	898:916	Therefore, the entrapment of κ-carrageenan within synthetic hydrogels improved both the swelling and the mechanical properties.
30606569	4	19	theme	mechanical	633:642	arg1	properties					644:653	The mechanical properties	629:653	The mechanical properties	629:653	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	0	20	theme	pectin	24:29	arg1	Entrapment					0:9	Entrapment	0:9	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.	0:129	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	3	21	theme	pores	622:626	arg1	formation					585:593	the formation	581:593	the formation of larger and more defined pores	581:626	The surface morphologies of the hydrogels showed that the pectin and κ-carrageenan favors the formation of larger and more defined pores.
30606569	1	22	theme	κ-carrageenan	206:218	arg1	properties					301:310	the mechanical properties	286:310	the mechanical properties	286:310	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	1	22	theme	κ-carrageenan	206:218	arg1	entrapment					172:181	the entrapment	168:181	the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling	168:280	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	6	23	theme	hydrogels	1004:1012	arg1	biocompatibility					980:995	The biocompatibility	976:995	The biocompatibility of the hydrogels	976:1012	The biocompatibility of the hydrogels was evaluated with in vitro cytotoxicity assays and the results indicated that they could be considered as candidates for biomedical use.
30606569	0	24	theme	κ-carrageenan	34:46	arg1	Entrapment					0:9	Entrapment	0:9	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.	0:129	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	6	25	dep	in	1033:1034	arg1	vitro					1036:1040	vitro	1036:1040	vitro	1036:1040	The biocompatibility of the hydrogels was evaluated with in vitro cytotoxicity assays and the results indicated that they could be considered as candidates for biomedical use.
30606569	4	26	theme	load	842:845	arg1	400 N					821:825	400 N	821:825	400 N of compression load	821:845	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	4	27	theme	mechanical	704:713	arg1	properties					715:724	the mechanical properties	700:724	the mechanical properties	700:724	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	2	28	theme	water	344:348	arg1	uptake					350:355	the water uptake	340:355	the water uptake (WU) of κ-carrageenan and chitosan hydrogels	340:400	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	28	theme	water	344:348	arg1	3.5					413:415	3.5	413:415	3.5	413:415	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	28	theme	water	344:348	arg1	WU					358:359	WU	358:359	WU	358:359	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	4	29	theme	synthetic	790:798	arg1	hydrogel					800:807	the synthetic hydrogel	786:807	the synthetic hydrogel reaching up 400 N of compression load	786:845	The mechanical properties indicated that the pectin increased slightly the mechanical properties and the κ-carrageenan improves the mechanical properties of the synthetic hydrogel reaching up 400 N of compression load.
30606569	3	30	theme	defined	614:620	arg1	pores					622:626	larger and more defined pores	598:626	larger and more defined pores	598:626	The surface morphologies of the hydrogels showed that the pectin and κ-carrageenan favors the formation of larger and more defined pores.
30606569	1	31	theme	Composite	131:139	arg1	hydrogels					141:149	Composite hydrogels	131:149	Composite hydrogels	131:149	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	1	32	theme	methacrylate-based	227:244	arg1	hydrogels					246:254	methacrylate-based hydrogels	227:254	methacrylate-based hydrogels	227:254	Composite hydrogels were obtained by the entrapment of chitosan, pectin or κ-carrageenan within methacrylate-based hydrogels to improve their swelling and the mechanical properties.
30606569	0	33	theme	based	68:72	arg1	hydrogels					74:82	methacrylate based hydrogels	55:82	methacrylate based hydrogels	55:82	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	0	34	from	Effect	85:90	arg1	swelling					95:102	swelling	95:102	swelling	95:102	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	0	34	from	Effect	85:90	arg1	properties					119:128	mechanical properties	108:128	mechanical properties	108:128	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	3	35	theme	larger	598:603	arg1	pores					622:626	larger and more defined pores	598:626	larger and more defined pores	598:626	The surface morphologies of the hydrogels showed that the pectin and κ-carrageenan favors the formation of larger and more defined pores.
30606569	2	36	theme	κ-carrageenan	365:377	arg1	uptake					350:355	the water uptake	340:355	the water uptake (WU) of κ-carrageenan and chitosan hydrogels	340:400	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	36	theme	κ-carrageenan	365:377	arg1	3.5					413:415	3.5	413:415	3.5	413:415	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	2	36	theme	κ-carrageenan	365:377	arg1	WU					358:359	WU	358:359	WU	358:359	The results indicated that the water uptake (WU) of κ-carrageenan and chitosan hydrogels were until 3.5 and 2.2 times higher than the WU of the synthetic hydrogel, respectively.
30606569	0	37	theme	methacrylate	55:66	arg1	hydrogels					74:82	methacrylate based hydrogels	55:82	methacrylate based hydrogels	55:82	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	0	38	dep	Entrapment	0:9	arg1	Effect					85:90	Effect	85:90	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.	0:129	Entrapment of chitosan, pectin or κ-carrageenan within methacrylate based hydrogels: Effect on swelling and mechanical properties.
30606569	6	39	theme	biomedical	1136:1145	arg1	use					1147:1149	biomedical use	1136:1149	biomedical use	1136:1149	The biocompatibility of the hydrogels was evaluated with in vitro cytotoxicity assays and the results indicated that they could be considered as candidates for biomedical use.
30606569	6	40	theme	cytotoxicity	1042:1053	arg1	assays					1055:1060	in vitro cytotoxicity assays	1033:1060	in vitro cytotoxicity assays	1033:1060	The biocompatibility of the hydrogels was evaluated with in vitro cytotoxicity assays and the results indicated that they could be considered as candidates for biomedical use.
30710585	7	0	theme	excellent	1123:1131	arg1	activity					1147:1154	an excellent antibacterial activity	1120:1154	an excellent antibacterial activity	1120:1154	Furthermore, PSP-AgNPs were found to have an excellent antibacterial activity and biocompatibility.
30710585	3	1	theme	gel	522:524	arg1	chromatography					537:550	gel permeation chromatography	522:550	gel permeation chromatography	522:550	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	5	2	theme	AgS	741:743	arg1	bonds					745:749	AgS bonds	741:749	AgS bonds	741:749	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	0	3	theme	abalone	94:100	arg1	viscera					102:108	abalone viscera	94:108	abalone viscera	94:108	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	7	4	theme	antibacterial	1133:1145	arg1	activity					1147:1154	an excellent antibacterial activity	1120:1154	an excellent antibacterial activity	1120:1154	Furthermore, PSP-AgNPs were found to have an excellent antibacterial activity and biocompatibility.
30710585	3	5	theme	permeation	526:535	arg1	chromatography					537:550	gel permeation chromatography	522:550	gel permeation chromatography	522:550	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	0	6	theme	antibacterial	114:126	arg1	activity					128:135	antibacterial activity	114:135	antibacterial activity	114:135	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	8	7	theme	proposed	1182:1189	arg1	synthesis					1191:1199	The proposed synthesis	1178:1199	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility	1178:1279	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	6	8	theme	AgNPs	905:909	arg1	capping					894:900	Such capping	889:900	Such capping of AgNPs by PSP	889:916	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	6	9	theme	stable	937:942	arg1	dispersion					944:953	the stable dispersion	933:953	the stable dispersion of PSP-AgNP composites	933:976	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	5	10	theme	transmission	855:866	arg1	microscopy					877:886	scanning transmission electron microscopy	846:886	scanning transmission electron microscopy	846:886	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	5	11	dep	transform	775:783	arg1	infrared					785:792	infrared	785:792	transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy	775:886	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	6	12	theme	multiple	1051:1058	arg1	scattering					1066:1075	multiple light scattering	1051:1075	multiple light scattering	1051:1075	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	8	13	theme	likely	1292:1297	arg1	benefit					1299:1305	likely benefit	1292:1305	likely benefit in the field of life science and technology	1292:1349	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	1	14	theme	antibacterial	195:207	arg1	nanoparticles					156:168	Silver nanoparticles	149:168	Silver nanoparticles (AgNPs)	149:176	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	1	14	theme	antibacterial	195:207	arg1	agents					209:214	eco-friendly antibacterial agents	182:214	eco-friendly antibacterial agents	182:214	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	3	15	theme	polysaccharide-protein	363:384	arg1	PSP					395:397	PSP	395:397	PSP	395:397	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	3	15	theme	polysaccharide-protein	363:384	arg1	complex					386:392	a polysaccharide-protein complex	361:392	a polysaccharide-protein complex (PSP)	361:398	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	5	16	theme	electron	868:875	arg1	microscopy					877:886	scanning transmission electron microscopy	846:886	scanning transmission electron microscopy	846:886	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	6	17	theme	Such	889:892	arg1	capping					894:900	Such capping	889:900	Such capping of AgNPs by PSP	889:916	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	0	18	dep	viscera	102:108	arg1	evaluation					137:146	evaluation	137:146	evaluation	137:146	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	3	19	theme	abalone	442:448	arg1	viscera					431:437	viscera	431:437	viscera of abalone	431:448	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	8	20	theme	AgNPs	1204:1208	arg1	synthesis					1191:1199	The proposed synthesis	1178:1199	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility	1178:1279	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	6	21	theme	room	981:984	arg1	temperature					986:996	room temperature	981:996	room temperature for 12 months	981:1010	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	5	22	dep	Fourier	767:773	arg1	transform					775:783	transform	775:783	transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy	775:886	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	2	23	theme	AgNPs	333:337	arg1	development					304:314	the development	300:314	the development of highly stable AgNPs	300:337	Therefore, the development of highly stable AgNPs is desirable.
30710585	2	23	theme	AgNPs	333:337	arg1	desirable					342:350	desirable	342:350	desirable	342:350	Therefore, the development of highly stable AgNPs is desirable.
30710585	8	24	from	benefit	1299:1305	arg1	field					1314:1318	the field	1310:1318	the field of life science and technology	1310:1349	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	0	25	theme	stable	22:27	arg1	nanoparticles					36:48	highly stable silver nanoparticles	15:48	highly stable silver nanoparticles	15:48	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	8	26	theme	antibacterial	1220:1232	arg1	activity					1234:1241	high antibacterial activity	1215:1241	high antibacterial activity	1215:1241	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	2	27	theme	stable	326:331	arg1	AgNPs					333:337	highly stable AgNPs	319:337	highly stable AgNPs	319:337	Therefore, the development of highly stable AgNPs is desirable.
30710585	3	28	theme	hydrolysis	485:494	arg1	combination					460:470	a combination	458:470	a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography	458:550	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	5	29	theme	photoelectron	814:826	arg1	spectroscopy					828:839	X-ray photoelectron spectroscopy	808:839	X-ray photoelectron spectroscopy	808:839	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	6	30	theme	composites	967:976	arg1	dispersion					944:953	the stable dispersion	933:953	the stable dispersion of PSP-AgNP composites	933:976	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	0	31	from	viscera	102:108	arg1	complexes					79:87	polysaccharide-protein complexes	56:87	polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation	56:146	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	1	32	theme	eco-friendly	182:193	arg1	nanoparticles					156:168	Silver nanoparticles	149:168	Silver nanoparticles (AgNPs)	149:176	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	1	32	theme	eco-friendly	182:193	arg1	agents					209:214	eco-friendly antibacterial agents	182:214	eco-friendly antibacterial agents	182:214	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	5	33	theme	X-ray	808:812	arg1	spectroscopy					828:839	X-ray photoelectron spectroscopy	808:839	X-ray photoelectron spectroscopy	808:839	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	0	34	theme	nanoparticles	36:48	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of highly stable silver nanoparticles	0:48	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	6	35	theme	PSP-AgNP	958:965	arg1	composites					967:976	PSP-AgNP composites	958:976	PSP-AgNP composites	958:976	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	0	36	theme	silver	29:34	arg1	nanoparticles					36:48	highly stable silver nanoparticles	15:48	highly stable silver nanoparticles	15:48	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	4	37	theme	capping	647:653	arg1	PSP					625:627	PSP	625:627	PSP	625:627	Furthermore, highly stable AgNPs were successfully synthesized by using PSP as a reducing and capping agent in situ.
30710585	4	37	theme	capping	647:653	arg1	agent					655:659	a reducing and capping agent	632:659	agent	655:659	Furthermore, highly stable AgNPs were successfully synthesized by using PSP as a reducing and capping agent in situ.
30710585	3	38	theme	enzymatic	475:483	arg1	hydrolysis					485:494	enzymatic hydrolysis	475:494	enzymatic hydrolysis	475:494	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	0	39	theme	polysaccharide-protein	56:77	arg1	complexes					79:87	polysaccharide-protein complexes	56:87	polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation	56:146	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	5	40	theme	AgN	732:734	arg1	formation					714:722	the formation	710:722	the formation of AgO, AgN, and AgS bonds	710:749	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	4	41	theme	stable	573:578	arg1	AgNPs					580:584	highly stable AgNPs	566:584	highly stable AgNPs	566:584	Furthermore, highly stable AgNPs were successfully synthesized by using PSP as a reducing and capping agent in situ.
30710585	7	42	contain	have	1115:1118	arg2	activity					1147:1154	an excellent antibacterial activity	1120:1154	an excellent antibacterial activity	1120:1154	Furthermore, PSP-AgNPs were found to have an excellent antibacterial activity and biocompatibility.
30710585	7	42	contain	have	1115:1118	arg1	PSP-AgNPs					1091:1099	PSP-AgNPs	1091:1099	PSP-AgNPs	1091:1099	Furthermore, PSP-AgNPs were found to have an excellent antibacterial activity and biocompatibility.
30710585	7	42	contain	have	1115:1118	arg2	biocompatibility					1160:1175	biocompatibility	1160:1175	biocompatibility	1160:1175	Furthermore, PSP-AgNPs were found to have an excellent antibacterial activity and biocompatibility.
30710585	1	43	theme	Silver	149:154	arg1	nanoparticles					156:168	Silver nanoparticles	149:168	Silver nanoparticles (AgNPs)	149:176	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	1	43	theme	Silver	149:154	arg1	agents					209:214	eco-friendly antibacterial agents	182:214	eco-friendly antibacterial agents	182:214	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	1	43	theme	Silver	149:154	arg1	AgNPs					171:175	AgNPs	171:175	AgNPs	171:175	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	0	44	from	activity	128:135	arg1	complexes					79:87	polysaccharide-protein complexes	56:87	polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation	56:146	Fabrication of highly stable silver nanoparticles using polysaccharide-protein complexes from abalone viscera and antibacterial activity evaluation.
30710585	8	45	dep	life	1323:1326	arg1	technology					1340:1349	technology	1340:1349	technology	1340:1349	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	8	45	dep	life	1323:1326	arg1	science					1328:1334	science	1328:1334	science	1328:1334	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	8	46	with	AgNPs	1204:1208	arg1	dispersibility					1244:1257	dispersibility	1244:1257	dispersibility	1244:1257	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	8	46	with	AgNPs	1204:1208	arg1	biocompatibility					1264:1279	biocompatibility	1264:1279	biocompatibility	1264:1279	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	8	46	with	AgNPs	1204:1208	arg1	activity					1234:1241	high antibacterial activity	1215:1241	high antibacterial activity	1215:1241	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	3	47	theme	membrane	497:504	arg1	filtration					506:515	membrane filtration	497:515	membrane filtration	497:515	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	4	48	theme	reducing	634:641	arg1	PSP					625:627	PSP	625:627	PSP	625:627	Furthermore, highly stable AgNPs were successfully synthesized by using PSP as a reducing and capping agent in situ.
30710585	4	48	theme	reducing	634:641	arg1	agent					655:659	a reducing and capping agent	632:659	agent	655:659	Furthermore, highly stable AgNPs were successfully synthesized by using PSP as a reducing and capping agent in situ.
30710585	3	49	theme	filtration	506:515	arg1	combination					460:470	a combination	458:470	a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography	458:550	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	8	50	theme	life	1323:1326	arg1	field					1314:1318	the field	1310:1318	the field of life science and technology	1310:1349	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	5	51	theme	bonds	745:749	arg1	formation					714:722	the formation	710:722	the formation of AgO, AgN, and AgS bonds	710:749	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	5	52	theme	scanning	846:853	arg1	microscopy					877:886	scanning transmission electron microscopy	846:886	scanning transmission electron microscopy	846:886	AgNPs were firmly capped by PSP through the formation of AgO, AgN, and AgS bonds, as observed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning transmission electron microscopy.
30710585	6	53	theme	light	1060:1064	arg1	scattering					1066:1075	multiple light scattering	1051:1075	multiple light scattering	1051:1075	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
30710585	3	54	theme	chromatography	537:550	arg1	combination					460:470	a combination	458:470	a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography	458:550	Herein, a polysaccharide-protein complex (PSP) was successfully obtained from viscera of abalone through a combination of enzymatic hydrolysis, membrane filtration, and gel permeation chromatography.
30710585	1	55	theme	facile	251:256	arg1	aggregation					258:268	their facile aggregation	245:268	their facile aggregation	245:268	Silver nanoparticles (AgNPs) are eco-friendly antibacterial agents, yet their use is limited by their facile aggregation and precipitation.
30710585	8	56	theme	high	1215:1218	arg1	activity					1234:1241	high antibacterial activity	1215:1241	high antibacterial activity	1215:1241	The proposed synthesis of AgNPs with high antibacterial activity, dispersibility, and biocompatibility will be of likely benefit in the field of life science and technology.
30710585	6	57	theme	visual	1029:1034	arg1	inspection					1036:1045	visual inspection	1029:1045	visual inspection	1029:1045	Such capping of AgNPs by PSP contributed to the stable dispersion of PSP-AgNP composites at room temperature for 12 months, as evidenced by visual inspection and multiple light scattering.
31004640	8	0	theme	Further	1122:1128	arg1	findings					1130:1137	Further findings	1122:1137	Further findings	1122:1137	Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4.
31004640	10	1	theme	D.	1498:1499	arg1	effects					1487:1493	the immunomodulating effects	1466:1493	the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons	1466:1577	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	9	2	theme	ERK	1294:1296	arg1	DvP-1					1251:1255	DvP-1	1251:1255	DvP-1	1251:1255	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	2	theme	ERK	1294:1296	arg1	agonist					1274:1280	a TLR4 agonist	1267:1280	a TLR4 agonist	1267:1280	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	2	theme	ERK	1294:1296	arg1	phosphorylation					1319:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	7	3	theme	zebrafish	1011:1019	arg1	model					1021:1025	a zebrafish model	1009:1025	a zebrafish model	1009:1025	By establishing a zebrafish model, we also found that DvP-1 could alleviate vinorelbine-induced decrease of macrophages in vivo.
31004640	3	4	from	D.	423:424	arg1	DvP-1					462:466	DvP-1	462:466	DvP-1 with molecular weights of 9.52 × 104 Da	462:506	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	3	4	from	D.	423:424	arg1	polysaccharide					403:416	a novel polysaccharide	395:416	a novel polysaccharide	395:416	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	7	5	theme	macrophages	1101:1111	arg1	decrease					1089:1096	vinorelbine-induced decrease	1069:1096	vinorelbine-induced decrease of macrophages	1069:1111	By establishing a zebrafish model, we also found that DvP-1 could alleviate vinorelbine-induced decrease of macrophages in vivo.
31004640	4	6	theme	molar	598:602	arg1	ration					604:609	the molar ration	594:609	the molar ration of 10.11: 1	594:621	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	5	7	theme	O-2	720:722	arg1	positions					740:748	the O-2, O-3 and/or O-6 positions	716:748	positions	740:748	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	9	8	theme	TLR4	1269:1272	arg1	phosphorylation					1319:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	8	theme	TLR4	1269:1272	arg1	agonist					1274:1280	a TLR4 agonist	1267:1280	a TLR4 agonist	1267:1280	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	8	theme	TLR4	1269:1272	arg1	DvP-1					1251:1255	DvP-1	1251:1255	DvP-1	1251:1255	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	7	9	theme	vinorelbine-induced	1069:1087	arg1	decrease					1089:1096	vinorelbine-induced decrease	1069:1096	vinorelbine-induced decrease of macrophages	1069:1111	By establishing a zebrafish model, we also found that DvP-1 could alleviate vinorelbine-induced decrease of macrophages in vivo.
31004640	4	10	theme	10.11	614:618	arg1	ration					604:609	the molar ration	594:609	the molar ration of 10.11: 1	594:621	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	0	11	theme	activating	79:88	arg1	macrophages					90:100	activating macrophages	79:100	activating macrophages	79:100	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	9	12	theme	NFκB	1374:1377	arg1	pathways					1389:1396	NFκB signaling pathways	1374:1396	NFκB signaling pathways downstream of TLR4	1374:1415	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	5	13	theme	O-3	725:727	arg1	positions					740:748	the O-2, O-3 and/or O-6 positions	716:748	positions	740:748	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	5	14	theme	main	628:631	arg1	linkages					644:651	The main glycosidic linkages	624:651	The main glycosidic linkages	624:651	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	5	14	theme	main	628:631	arg1	β-1					658:660	β-1	658:660	β-1	658:660	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	1	15	theme	nutraceutical	163:175	arg1	devonianum					114:123	Dendrobium devonianum	103:123	Dendrobium devonianum	103:123	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	15	theme	nutraceutical	163:175	arg1	products					177:184	nutraceutical products	163:184	nutraceutical products	163:184	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	3	16	theme	characteristic	350:363	arg1	identification					365:378	characteristic identification	350:378	characteristic identification	350:378	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	0	17	from	devonianum	39:48	arg1	polysaccharide					8:21	A novel polysaccharide	0:21	A novel polysaccharide from Dendrobium devonianum	0:48	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	0	17	from	devonianum	39:48	arg1	agonist					67:73	a TLR4 agonist	60:73	a TLR4 agonist for activating macrophages	60:100	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	5	18	theme	glycosidic	633:642	arg1	linkages					644:651	The main glycosidic linkages	624:651	The main glycosidic linkages	624:651	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	5	18	theme	glycosidic	633:642	arg1	β-1					658:660	β-1	658:660	β-1	658:660	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	10	19	theme	immunocompromised	1553:1569	arg1	persons					1571:1577	immunocompromised persons	1553:1577	immunocompromised persons	1553:1577	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	9	20	theme	pathways	1389:1396	arg1	activation					1351:1360	the activation	1347:1360	the activation of MAPK and NFκB signaling pathways downstream of TLR4	1347:1415	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	0	21	theme	novel	2:6	arg1	polysaccharide					8:21	A novel polysaccharide	0:21	A novel polysaccharide from Dendrobium devonianum	0:48	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	0	21	theme	novel	2:6	arg1	agonist					67:73	a TLR4 agonist	60:73	a TLR4 agonist for activating macrophages	60:100	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	6	22	theme	morphologic	854:864	arg1	change					866:871	morphologic change	854:871	morphologic change	854:871	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	3	23	dep	D.	423:424	arg1	devonianum					426:435	D. devonianum	423:435	D. devonianum	423:435	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	0	24	theme	TLR4	62:65	arg1	agonist					67:73	a TLR4 agonist	60:73	a TLR4 agonist for activating macrophages	60:100	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	0	24	theme	TLR4	62:65	arg1	polysaccharide					8:21	A novel polysaccharide	0:21	A novel polysaccharide from Dendrobium devonianum	0:48	A novel polysaccharide from Dendrobium devonianum serves as a TLR4 agonist for activating macrophages.
31004640	1	25	used	used	134:137	arg2	devonianum					114:123	Dendrobium devonianum	103:123	Dendrobium devonianum	103:123	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	25	used	used	134:137	arg2	medicines					149:157	herbal medicines	142:157	herbal medicines	142:157	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	25	used	used	134:137	arg2	products					177:184	nutraceutical products	163:184	nutraceutical products	163:184	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	3	26	theme	novel	397:401	arg1	DvP-1					462:466	DvP-1	462:466	DvP-1 with molecular weights of 9.52 × 104 Da	462:506	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	3	26	theme	novel	397:401	arg1	polysaccharide					403:416	a novel polysaccharide	395:416	a novel polysaccharide	395:416	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	6	27	theme	macrophages	807:817	arg1	activation					793:802	the activation	789:802	the activation of macrophages	789:817	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	1	28	theme	ancient	192:198	arg1	time					200:203	ancient time	192:203	ancient time in China	192:212	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	4	29	from	d-glucose	581:589	arg1	ration					604:609	the molar ration	594:609	the molar ration of 10.11: 1	594:621	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	9	30	theme	JNK	1299:1301	arg1	DvP-1					1251:1255	DvP-1	1251:1255	DvP-1	1251:1255	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	30	theme	JNK	1299:1301	arg1	agonist					1274:1280	a TLR4 agonist	1267:1280	a TLR4 agonist	1267:1280	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	30	theme	JNK	1299:1301	arg1	phosphorylation					1319:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	5	31	theme	acetyl	699:704	arg1	groups					706:711	acetyl groups	699:711	acetyl groups at the O-2, O-3 and/or O-6 positions	699:748	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	9	32	theme	signaling	1379:1387	arg1	pathways					1389:1396	NFκB signaling pathways	1374:1396	NFκB signaling pathways downstream of TLR4	1374:1415	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	6	33	dep	stimulate	779:787	arg1	enhancing					944:952	enhancing	944:952	enhancing the pinocytic activity of macrophages	944:990	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	6	33	dep	stimulate	779:787	arg1	promoting					882:890	promoting	882:890	promoting the production of cytokines TNF-α, IL-6 and NO,	882:938	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	9	34	theme	TLR4	1412:1415	arg1	downstream					1398:1407	downstream	1398:1407	downstream	1398:1407	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	3	35	with	DvP-1	462:466	arg1	weights					483:489	molecular weights	473:489	molecular weights of 9.52 × 104 Da	473:506	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	1	36	theme	Dendrobium	103:112	arg1	devonianum					114:123	Dendrobium devonianum	103:123	Dendrobium devonianum	103:123	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	36	theme	Dendrobium	103:112	arg1	medicines					149:157	herbal medicines	142:157	herbal medicines	142:157	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	36	theme	Dendrobium	103:112	arg1	products					177:184	nutraceutical products	163:184	nutraceutical products	163:184	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	11	37	theme	diseases	1667:1674	arg1	treatment					1632:1640	the treatment	1628:1640	the treatment of cancer and infectious diseases caused by TLR4 dysfunction	1628:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	3	38	theme	molecular	473:481	arg1	weights					483:489	molecular weights	473:489	molecular weights of 9.52 × 104 Da	473:506	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	4	39	from	D-mannose	567:575	arg1	ration					604:609	the molar ration	594:609	the molar ration of 10.11: 1	594:621	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	9	40	theme	p38	1304:1306	arg1	DvP-1					1251:1255	DvP-1	1251:1255	DvP-1	1251:1255	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	40	theme	p38	1304:1306	arg1	agonist					1274:1280	a TLR4 agonist	1267:1280	a TLR4 agonist	1267:1280	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	40	theme	p38	1304:1306	arg1	phosphorylation					1319:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	11	41	theme	infectious	1656:1665	arg1	diseases					1667:1674	infectious diseases	1656:1674	infectious diseases caused by TLR4 dysfunction	1656:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	2	42	theme	chemical	228:235	arg1	composition					237:247	its chemical composition	224:247	its chemical composition	224:247	However, its chemical composition and pharmacological mechanisms are not fully known.
31004640	5	43	theme	O-6	736:738	arg1	positions					740:748	the O-2, O-3 and/or O-6 positions	716:748	positions	740:748	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	6	44	theme	pinocytic	958:966	arg1	activity					968:975	the pinocytic activity	954:975	the pinocytic activity of macrophages	954:990	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	8	45	theme	several	1190:1196	arg1	receptors					1208:1216	several toll-like receptors	1190:1216	several toll-like receptors (TLRs)	1190:1223	Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4.
31004640	8	45	theme	several	1190:1196	arg1	TLRs					1219:1222	TLRs	1219:1222	TLRs	1219:1222	Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4.
31004640	4	46	theme	homogeneous	520:530	arg1	heteropolysaccharide					532:551	a homogeneous heteropolysaccharide	518:551	a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1	518:621	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	4	46	theme	homogeneous	520:530	arg1	DvP-1					509:513	DvP-1	509:513	DvP-1	509:513	DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1.
31004640	10	47	theme	Chinese	1515:1521	arg1	medicines					1523:1531	Chinese medicines	1515:1531	Chinese medicines	1515:1531	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	10	48	dep	D.	1498:1499	arg1	devonianum					1501:1510	D. devonianum	1498:1510	D. devonianum	1498:1510	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	10	49	theme	health	1536:1541	arg1	foods					1543:1547	health foods	1536:1547	health foods	1536:1547	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	3	50	theme	present	304:310	arg1	studies					312:318	present studies	304:318	present studies	304:318	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	11	51	theme	cancer	1645:1650	arg1	treatment					1632:1640	the treatment	1628:1640	the treatment of cancer and infectious diseases caused by TLR4 dysfunction	1628:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	11	52	theme	DvP-1	1618:1622	arg1	value					1609:1613	the medicinal value	1595:1613	the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction	1595:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	3	53	theme	9.52 × 104 Da	494:506	arg1	weights					483:489	molecular weights	473:489	molecular weights of 9.52 × 104 Da	473:506	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	11	54	theme	medicinal	1599:1607	arg1	value					1609:1613	the medicinal value	1595:1613	the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction	1595:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	9	55	theme	IκB-α	1313:1317	arg1	DvP-1					1251:1255	DvP-1	1251:1255	DvP-1	1251:1255	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	55	theme	IκB-α	1313:1317	arg1	agonist					1274:1280	a TLR4 agonist	1267:1280	a TLR4 agonist	1267:1280	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	9	55	theme	IκB-α	1313:1317	arg1	phosphorylation					1319:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	ERK, JNK, p38, and IκB-α phosphorylation	1294:1333	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	10	56	theme	immunomodulating	1470:1485	arg1	effects					1487:1493	the immunomodulating effects	1466:1493	the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons	1466:1577	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	1	57	from	time	200:203	arg1	China					208:212	China	208:212	China	208:212	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	6	58	theme	cytokines	910:918	arg1	production					896:905	the production	892:905	the production of cytokines TNF-α, IL-6 and NO,	892:938	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	11	59	theme	TLR4	1686:1689	arg1	dysfunction					1691:1701	TLR4 dysfunction	1686:1701	TLR4 dysfunction	1686:1701	They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.
31004640	9	60	theme	MAPK	1365:1368	arg1	activation					1351:1360	the activation	1347:1360	the activation of MAPK and NFκB signaling pathways downstream of TLR4	1347:1415	DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4.
31004640	2	61	theme	pharmacological	253:267	arg1	mechanisms					269:278	pharmacological mechanisms	253:278	pharmacological mechanisms	253:278	However, its chemical composition and pharmacological mechanisms are not fully known.
31004640	8	62	theme	toll-like	1198:1206	arg1	receptors					1208:1216	several toll-like receptors	1190:1216	several toll-like receptors (TLRs)	1190:1223	Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4.
31004640	8	62	theme	toll-like	1198:1206	arg1	TLRs					1219:1222	TLRs	1219:1222	TLRs	1219:1222	Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4.
31004640	3	63	theme	chemical	324:331	arg1	purification					333:344	chemical purification	324:344	chemical purification	324:344	In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da.
31004640	5	64	from	positions	740:748	arg1	groups					706:711	acetyl groups	699:711	acetyl groups at the O-2, O-3 and/or O-6 positions	699:748	The main glycosidic linkages were β-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions.
31004640	6	65	theme	macrophages	980:990	arg1	activity					968:975	the pinocytic activity	954:975	the pinocytic activity of macrophages	954:990	DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages.
31004640	10	66	from	effects	1487:1493	arg1	medicines					1523:1531	Chinese medicines	1515:1531	Chinese medicines	1515:1531	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	10	66	from	effects	1487:1493	arg1	foods					1543:1547	health foods	1536:1547	health foods	1536:1547	These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons.
31004640	1	67	theme	herbal	142:147	arg1	devonianum					114:123	Dendrobium devonianum	103:123	Dendrobium devonianum	103:123	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31004640	1	67	theme	herbal	142:147	arg1	medicines					149:157	herbal medicines	142:157	herbal medicines	142:157	Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China.
31094393	2	0	theme	product	491:497	arg1	substrate					469:477	donor substrate	463:477	donor substrate (up to 8 mM product titre)	463:504	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	0	theme	product	491:497	arg1	titre					499:503	up to 8 mM product titre	480:503	up to 8 mM product titre	480:503	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	1	gly	β-1,3-glycosylation	222:240	arg1	lactose					245:251	lactose	245:251	lactose	245:251	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	2	dep	8	486:486	arg1	to					483:484	to	483:484	to	483:484	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	0	3	from	variants	58:65	arg1	bifidum					114:120	Bifidobacterium bifidum	98:120	Bifidobacterium bifidum	98:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	2	4	theme	%	452:452	arg1	yield					454:458	20-60% yield	447:458	20-60% yield of donor substrate (up to 8 mM product titre)	447:504	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	5	theme	donor	202:206	arg1	substrate					208:216	a donor substrate	200:216	a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	200:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	5	theme	donor	202:206	arg1	1,2-oxazoline					137:149	Lacto-N-biose 1,2-oxazoline	123:149	Lacto-N-biose 1,2-oxazoline	123:149	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	6	theme	mM	488:489	arg1	substrate					469:477	donor substrate	463:477	donor substrate (up to 8 mM product titre)	463:504	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	6	theme	mM	488:489	arg1	titre					499:503	up to 8 mM product titre	480:503	up to 8 mM product titre	480:503	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	7	theme	substrate	469:477	arg1	yield					454:458	20-60% yield	447:458	20-60% yield of donor substrate (up to 8 mM product titre)	447:504	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	0	8	theme	Lacto-N-tetraose	0:15	arg1	synthesis					17:25	Lacto-N-tetraose synthesis	0:25	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.	0:121	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	2	9	theme	oligosaccharides	415:430	arg1	Lacto-N-tetraose					366:381	Lacto-N-tetraose	366:381	Lacto-N-tetraose	366:381	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	9	theme	oligosaccharides	415:430	arg1	structure					391:399	a core structure	384:399	a core structure of human milk oligosaccharides	384:430	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	0	10	theme	wild-type	30:38	arg1	variants					58:65	wild-type and glycosynthase variants	30:65	wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum	30:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	1	11	theme	Lacto-N-biose	123:135	arg1	substrate					208:216	a donor substrate	200:216	a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	200:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	11	theme	Lacto-N-biose	123:135	arg1	1,2-oxazoline					137:149	Lacto-N-biose 1,2-oxazoline	123:149	Lacto-N-biose 1,2-oxazoline	123:149	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	12	theme	control	546:552	arg1	degree					524:529	the degree	520:529	the degree of selectivity control by the enzyme used	520:571	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	13	theme	lactose	245:251	arg1	β-1,3-glycosylation					222:240	β-1,3-glycosylation	222:240	β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	222:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	0	14	theme	glycosynthase	44:56	arg1	variants					58:65	wild-type and glycosynthase variants	30:65	wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum	30:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	2	15	theme	selectivity	534:544	arg1	control					546:552	selectivity control	534:552	selectivity control	534:552	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	16	theme	20-60	447:451	arg1	%					452:452	%	452:452	%	452:452	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	17	theme	Bifidobacterium	322:336	arg1	β-N-hexosaminidase					346:363	Bifidobacterium bifidum β-N-hexosaminidase	322:363	Bifidobacterium bifidum β-N-hexosaminidase	322:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	18	theme	donor	463:467	arg1	substrate					469:477	donor substrate	463:477	donor substrate (up to 8 mM product titre)	463:504	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	18	theme	donor	463:467	arg1	titre					499:503	up to 8 mM product titre	480:503	up to 8 mM product titre	480:503	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	19	theme	bifidum	338:344	arg1	β-N-hexosaminidase					346:363	Bifidobacterium bifidum β-N-hexosaminidase	322:363	Bifidobacterium bifidum β-N-hexosaminidase	322:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	20	theme	core	386:389	arg1	Lacto-N-tetraose					366:381	Lacto-N-tetraose	366:381	Lacto-N-tetraose	366:381	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	2	20	theme	core	386:389	arg1	structure					391:399	a core structure	384:399	a core structure of human milk oligosaccharides	384:430	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	21	theme	β-N-hexosaminidase	346:363	arg1	variants					288:295	the wild-type and glycosynthase variants	256:295	the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	256:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	21	theme	β-N-hexosaminidase	346:363	arg1	Y419F					312:316	Y419F	312:316	Y419F	312:316	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	22	theme	wild-type	260:268	arg1	variants					288:295	the wild-type and glycosynthase variants	256:295	the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	256:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	22	theme	wild-type	260:268	arg1	Y419F					312:316	Y419F	312:316	Y419F	312:316	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	0	23	theme	β-N-hexosaminidase	74:91	arg1	variants					58:65	wild-type and glycosynthase variants	30:65	wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum	30:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	0	24	from	bifidum	114:120	arg1	variants					58:65	wild-type and glycosynthase variants	30:65	wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum	30:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	0	24	from	bifidum	114:120	arg1	β-N-hexosaminidase					74:91	the β-N-hexosaminidase	70:91	the β-N-hexosaminidase from Bifidobacterium bifidum	70:120	Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum.
31094393	2	25	theme	milk	410:413	arg1	oligosaccharides					415:430	human milk oligosaccharides	404:430	human milk oligosaccharides	404:430	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31094393	1	26	theme	glycosynthase	274:286	arg1	variants					288:295	the wild-type and glycosynthase variants	256:295	the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase	256:363	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	1	26	theme	glycosynthase	274:286	arg1	Y419F					312:316	Y419F	312:316	Y419F	312:316	Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
31094393	2	27	theme	human	404:408	arg1	oligosaccharides					415:430	human milk oligosaccharides	404:430	human milk oligosaccharides	404:430	Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
31826465	3	0	theme	hydrogen	504:511	arg1	bonds					513:517	the hydrogen bonds	500:517	the hydrogen bonds among RAs, OMP and PVA	500:540	The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA.
31826465	4	1	theme	RAs	569:571	arg1	content					558:564	An appropriate content	543:564	An appropriate content of RAs and OMP	543:579	An appropriate content of RAs and OMP could improve the film mechanical and barrier properties.
31826465	0	2	theme	rose	92:95	arg1	anthocyanins					97:108	rose anthocyanins	92:108	rose anthocyanins for shrimp freshness monitoring	92:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	6	3	theme	shrimp	810:815	arg1	freshness					817:825	shrimp freshness	810:825	shrimp freshness	810:825	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	6	4	theme	products	988:995	arg1	monitoring					966:975	freshness monitoring	956:975	freshness monitoring of aquatic products and meat foods	956:1010	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	0	5	theme	shrimp	114:119	arg1	monitoring					131:140	shrimp freshness monitoring	114:140	shrimp freshness monitoring	114:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	6	6	theme	naked	891:895	arg1	eye					897:899	naked eye	891:899	naked eye	891:899	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	5	7	theme	colorimetric	643:654	arg1	sensitive					722:730	sensitive	722:730	sensitive	722:730	The colorimetric film showed distinguishable color changes at pH 2-12 and was high sensitive to volatile ammonia.
31826465	5	7	theme	colorimetric	643:654	arg1	film					656:659	The colorimetric film	639:659	The colorimetric film	639:659	The colorimetric film showed distinguishable color changes at pH 2-12 and was high sensitive to volatile ammonia.
31826465	1	8	theme	polyvinyl	266:274	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	1	9	theme	colorimetric	159:170	arg1	film					172:175	a colorimetric film	157:175	a colorimetric film	157:175	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	1	10	theme	alcohol/okra	276:287	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	2	11	theme	non-Newtonian	411:423	arg1	fluid					425:429	non-Newtonian fluid	411:429	non-Newtonian fluid	411:429	The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid.
31826465	0	12	theme	freshness	121:129	arg1	monitoring					131:140	shrimp freshness monitoring	114:140	shrimp freshness monitoring	114:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	6	13	theme	PVA/OMP-RAs	772:782	arg1	film					764:767	The target film	753:767	The target film of PVA/OMP-RAs	753:782	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	3	14	theme	RAs	456:458	arg1	addition					436:443	The addition	432:443	The addition of OMP and RAs	432:458	The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA.
31826465	4	15	theme	appropriate	546:556	arg1	content					558:564	An appropriate content	543:564	An appropriate content of RAs and OMP	543:579	An appropriate content of RAs and OMP could improve the film mechanical and barrier properties.
31826465	0	16	theme	Colorimetric	0:11	arg1	film					13:16	Colorimetric film	0:16	Colorimetric film	0:16	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	1	17	theme	mucilage	289:296	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	1	18	theme	shrimp	194:199	arg1	monitoring					211:220	shrimp freshness monitoring	194:220	shrimp freshness monitoring	194:220	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	1	19	theme	polysaccharide	298:311	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	6	20	theme	target	757:762	arg1	film					764:767	The target film	753:767	The target film of PVA/OMP-RAs	753:782	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	1	21	theme	freshness	201:209	arg1	monitoring					211:220	shrimp freshness monitoring	194:220	shrimp freshness monitoring	194:220	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	0	22	theme	alcohol/okra	37:48	arg1	polysaccharide					59:72	polyvinyl alcohol/okra mucilage polysaccharide	27:72	polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring	27:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	1	23	theme	PVA/OMP	314:320	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	6	24	theme	color	848:852	arg1	changes					854:860	the color changes	844:860	the color changes	844:860	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	3	25	theme	OMP	448:450	arg1	addition					436:443	The addition	432:443	The addition of OMP and RAs	432:458	The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA.
31826465	0	26	theme	polyvinyl	27:35	arg1	polysaccharide					59:72	polyvinyl alcohol/okra mucilage polysaccharide	27:72	polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring	27:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	6	27	theme	intelligent	930:940	arg1	packaging					942:950	intelligent packaging	930:950	intelligent packaging	930:950	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	2	28	from	Newtonian	398:406	arg1	solution					384:391	the film-forming solution	367:391	the film-forming solution from Newtonian to non-Newtonian fluid	367:429	The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid.
31826465	4	29	theme	barrier	619:625	arg1	properties					627:636	the film mechanical and barrier properties	595:636	properties	627:636	An appropriate content of RAs and OMP could improve the film mechanical and barrier properties.
31826465	1	30	theme	composite	323:331	arg1	film					333:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film	266:336	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	6	31	from	potential	917:925	arg1	packaging					942:950	intelligent packaging	930:950	intelligent packaging	930:950	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	6	32	theme	freshness	956:964	arg1	monitoring					966:975	freshness monitoring	956:975	freshness monitoring of aquatic products and meat foods	956:1010	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	2	33	theme	OMP	355:357	arg1	presence					343:350	The presence	339:350	The presence of OMP	339:357	The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid.
31826465	0	34	theme	mucilage	50:57	arg1	polysaccharide					59:72	polyvinyl alcohol/okra mucilage polysaccharide	27:72	polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring	27:140	Colorimetric film based on polyvinyl alcohol/okra mucilage polysaccharide incorporated with rose anthocyanins for shrimp freshness monitoring.
31826465	5	35	theme	distinguishable	668:682	arg1	changes					690:696	distinguishable color changes	668:696	distinguishable color changes	668:696	The colorimetric film showed distinguishable color changes at pH 2-12 and was high sensitive to volatile ammonia.
31826465	6	36	theme	aquatic	980:986	arg1	products					988:995	aquatic products	980:995	aquatic products	980:995	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	5	37	theme	color	684:688	arg1	changes					690:696	distinguishable color changes	668:696	distinguishable color changes	668:696	The colorimetric film showed distinguishable color changes at pH 2-12 and was high sensitive to volatile ammonia.
31826465	6	38	theme	real	830:833	arg1	time					835:838	real time	830:838	real time	830:838	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	3	39	theme	PVA	489:491	arg1	crystalline					474:484	the crystalline	470:484	the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA	470:540	The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA.
31826465	1	40	theme	rose	239:242	arg1	RAs					258:260	RAs	258:260	RAs	258:260	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	1	40	theme	rose	239:242	arg1	anthocyanins					244:255	rose anthocyanins	239:255	rose anthocyanins (RAs)	239:261	In this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film.
31826465	5	41	theme	volatile	735:742	arg1	ammonia					744:750	volatile ammonia	735:750	volatile ammonia	735:750	The colorimetric film showed distinguishable color changes at pH 2-12 and was high sensitive to volatile ammonia.
31826465	6	42	theme	foods	1006:1010	arg1	monitoring					966:975	freshness monitoring	956:975	freshness monitoring of aquatic products and meat foods	956:1010	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
31826465	4	43	theme	OMP	577:579	arg1	content					558:564	An appropriate content	543:564	An appropriate content of RAs and OMP	543:579	An appropriate content of RAs and OMP could improve the film mechanical and barrier properties.
31826465	2	44	theme	film-forming	371:382	arg1	solution					384:391	the film-forming solution	367:391	the film-forming solution from Newtonian to non-Newtonian fluid	367:429	The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid.
31826465	6	45	theme	meat	1001:1004	arg1	foods					1006:1010	meat foods	1001:1010	meat foods	1001:1010	The target film of PVA/OMP-RAs could effectively monitor shrimp freshness in real time and the color changes were easily distinguished by naked eye, suggesting its potential in intelligent packaging for freshness monitoring of aquatic products and meat foods.
30393134	5	0	with	tract	895:899	arg1	pHs					916:918	different pHs	906:918	different pHs	906:918	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	4	1	theme	FA	614:615	arg1	bio-polymer					701:711	bio-polymer	701:711	bio-polymer	701:711	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	4	1	theme	FA	614:615	arg1	LDH-FA					635:640	FA intercalated LDH (LDH-FA)	614:641	FA intercalated LDH (LDH-FA)	614:641	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	7	2	theme	exchange	1350:1357	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	2	theme	exchange	1350:1357	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	2	theme	exchange	1350:1357	arg1	process					1359:1365	ion exchange process	1346:1365	ion exchange process	1346:1365	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	3	from	leaching	1417:1424	arg1	structure					1452:1460	LDH structure	1448:1460	LDH structure	1448:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	5	4	theme	FA	797:798	arg1	drug					800:803	FA drug	797:803	FA drug	797:803	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	0	5	dep	release	129:135	arg1	evaluation					151:160	evaluation	151:160	evaluation	151:160	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	7	6	theme	FA	1433:1434	arg1	anions					1436:1441	the FA anions	1429:1441	the FA anions from LDH structure	1429:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	6	7	theme	release	1009:1015	arg1	test					1017:1020	the release test	1005:1020	the pH-sensitive swelling property and also the release test	961:1020	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	7	8	theme	LDH	1368:1370	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	8	theme	LDH	1368:1370	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	8	theme	LDH	1368:1370	arg1	diffusion					1387:1395	LDH intra-particle diffusion	1368:1395	LDH intra-particle diffusion	1368:1395	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	1	9	with	carrier	284:290	arg1	coprecipitation					301:315	two coprecipitation and anion exchange methods	297:342	coprecipitation	301:315	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	9	with	carrier	284:290	arg1	methods					336:342	two coprecipitation and anion exchange methods	297:342	methods	336:342	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	3	10	theme	FA	483:484	arg1	loading					486:492	the FA loading	479:492	the FA loading in the structure of LDH	479:516	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	3	10	theme	FA	483:484	arg1	%					533:533	about 33.35%	522:533	about 33.35%	522:533	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	3	11	from	loading	486:492	arg1	structure					501:509	the structure	497:509	the structure of LDH	497:516	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	1	12	theme	anion	321:325	arg1	methods					336:342	two coprecipitation and anion exchange methods	297:342	methods	336:342	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	4	13	theme	different	663:671	arg1	weights					673:679	different weights	663:679	different weights of chitosan (Cs)	663:696	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	7	14	from	diffusion	1387:1395	arg1	structure					1452:1460	LDH structure	1448:1460	LDH structure	1448:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	1	15	theme	layered	245:251	arg1	LDH					271:273	LDH	271:273	LDH	271:273	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	15	theme	layered	245:251	arg1	hydroxide					260:268	layered double hydroxide	245:268	layered double hydroxide (LDH)	245:274	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	0	16	theme	Drug	124:127	arg1	release					129:135	Drug release	124:135	Drug release	124:135	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	1	17	theme	exchange	327:334	arg1	methods					336:342	two coprecipitation and anion exchange methods	297:342	methods	336:342	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	4	18	theme	chitosan	684:691	arg1	weights					673:679	different weights	663:679	different weights of chitosan (Cs)	663:696	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	2	19	theme	FA	362:363	arg1	drug					365:368	FA drug	362:368	FA drug	362:368	The existence of FA drug between the LDH layers was confirmed with FT-IR and XRD analyses.
30393134	7	20	theme	LDH	1448:1450	arg1	structure					1452:1460	LDH structure	1448:1460	LDH structure	1448:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	4	21	theme	nanocomposite	730:742	arg1	beads					744:748	the obtained nanocomposite beads	717:748	the obtained nanocomposite beads	717:748	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	5	22	from	hybrids	819:825	arg1	release					786:792	the release	782:792	the release of FA drug from obtained hybrids	782:825	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	5	23	theme	gastrointestinal	878:893	arg1	tract					895:899	the gastrointestinal tract	874:899	the gastrointestinal tract with different pHs	874:918	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	1	24	theme	folic	178:182	arg1	acid					184:187	folic acid	178:187	folic acid (FA) as drug model	178:206	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	24	theme	folic	178:182	arg1	FA					190:191	FA	190:191	FA	190:191	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	24	theme	folic	178:182	arg1	carrier					284:290	drug carrier	279:290	drug carrier with two coprecipitation and anion exchange methods	279:342	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	7	25	theme	ion	1346:1348	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	25	theme	ion	1346:1348	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	25	theme	ion	1346:1348	arg1	process					1359:1365	ion exchange process	1346:1365	ion exchange process	1346:1365	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	26	theme	kinetic	1166:1172	arg1	models					1174:1179	several well-known kinetic models	1147:1179	several well-known kinetic models	1147:1179	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	27	from	process	1359:1365	arg1	structure					1452:1460	LDH structure	1448:1460	LDH structure	1448:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	1	28	theme	double	253:258	arg1	LDH					271:273	LDH	271:273	LDH	271:273	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	28	theme	double	253:258	arg1	hydroxide					260:268	layered double hydroxide	245:268	layered double hydroxide (LDH)	245:274	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	0	29	dep	intercalated	93:104	arg1	mechanism					141:149	mechanism	141:149	mechanism	141:149	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	0	29	dep	intercalated	93:104	arg1	release					129:135	Drug release	124:135	Drug release	124:135	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	6	30	theme	swelling	978:985	arg1	property					987:994	the pH-sensitive swelling property	961:994	the pH-sensitive swelling property and also the release test	961:1020	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	7	31	theme	release	1259:1265	arg1	leaching					1417:1424	also concurrent leaching	1401:1424	also concurrent leaching of the FA anions from LDH structure	1401:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	31	theme	release	1259:1265	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	31	theme	release	1259:1265	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	31	theme	release	1259:1265	arg1	process					1359:1365	ion exchange process	1346:1365	ion exchange process	1346:1365	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	31	theme	release	1259:1265	arg1	diffusion					1387:1395	LDH intra-particle diffusion	1368:1395	LDH intra-particle diffusion	1368:1395	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	1	32	theme	hydroxide	260:268	arg1	structure					232:240	the structure	228:240	the structure of layered double hydroxide (LDH)	228:274	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	4	33	theme	obtained	721:728	arg1	beads					744:748	the obtained nanocomposite beads	717:748	the obtained nanocomposite beads	717:748	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	2	34	theme	LDH	382:384	arg1	layers					386:391	the LDH layers	378:391	the LDH layers	378:391	The existence of FA drug between the LDH layers was confirmed with FT-IR and XRD analyses.
30393134	7	35	theme	flat	1309:1312	arg1	surface					1314:1320	flat surface	1309:1320	flat surface	1309:1320	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	6	36	theme	FA	1084:1085	arg1	drug					1087:1090	FA drug	1084:1090	FA drug	1084:1090	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	5	37	theme	drug	800:803	arg1	release					786:792	the release	782:792	the release of FA drug from obtained hybrids	782:825	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	0	38	theme	bio-nanocomposite	49:65	arg1	beads					67:71	pH-sensitive bio-nanocomposite beads	36:71	pH-sensitive bio-nanocomposite beads havening folic acid	36:91	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	5	39	theme	obtained	810:817	arg1	hybrids					819:825	obtained hybrids	810:825	obtained hybrids	810:825	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	7	40	from	structure	1452:1460	arg1	anions					1436:1441	the FA anions	1429:1441	the FA anions from LDH structure	1429:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	40	from	structure	1452:1460	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	40	from	structure	1452:1460	arg1	process					1359:1365	ion exchange process	1346:1365	ion exchange process	1346:1365	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	40	from	structure	1452:1460	arg1	leaching					1417:1424	also concurrent leaching	1401:1424	also concurrent leaching of the FA anions from LDH structure	1401:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	40	from	structure	1452:1460	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	40	from	structure	1452:1460	arg1	diffusion					1387:1395	LDH intra-particle diffusion	1368:1395	LDH intra-particle diffusion	1368:1395	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	3	41	theme	anion	570:574	arg1	exchange					576:583	anion exchange	570:583	anion exchange	570:583	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	6	42	theme	concentration	1126:1138	arg1	addition					1111:1118	addition	1111:1118	addition of Cs concentration	1111:1138	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	0	43	theme	pH-sensitive	36:47	arg1	beads					67:71	pH-sensitive bio-nanocomposite beads	36:71	pH-sensitive bio-nanocomposite beads havening folic acid	36:91	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	2	44	theme	XRD	422:424	arg1	analyses					426:433	XRD analyses	422:433	XRD analyses	422:433	The existence of FA drug between the LDH layers was confirmed with FT-IR and XRD analyses.
30393134	7	45	theme	FA	1217:1218	arg1	mechanism					1228:1236	the FA release mechanism	1213:1236	the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure	1213:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	6	46	theme	Cs	1123:1124	arg1	concentration					1126:1138	Cs concentration	1123:1138	Cs concentration	1123:1138	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	1	47	theme	drug	197:200	arg1	model					202:206	drug model	197:206	drug model	197:206	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	3	48	theme	coprecipitation	550:564	arg1	methods					585:591	coprecipitation and anion exchange methods	550:591	coprecipitation and anion exchange methods	550:591	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	3	49	theme	LDH	514:516	arg1	structure					501:509	the structure	497:509	the structure of LDH	497:516	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	4	50	theme	LDH	630:632	arg1	bio-polymer					701:711	bio-polymer	701:711	bio-polymer	701:711	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	4	50	theme	LDH	630:632	arg1	LDH-FA					635:640	FA intercalated LDH (LDH-FA)	614:641	FA intercalated LDH (LDH-FA)	614:641	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	0	51	theme	beads	67:71	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	0	51	theme	beads	67:71	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	1	52	theme	drug	279:282	arg1	acid					184:187	folic acid	178:187	folic acid (FA) as drug model	178:206	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	1	52	theme	drug	279:282	arg1	carrier					284:290	drug carrier	279:290	drug carrier with two coprecipitation and anion exchange methods	279:342	In this paper, folic acid (FA) as drug model was intercalated in the structure of layered double hydroxide (LDH) as drug carrier with two coprecipitation and anion exchange methods.
30393134	7	53	theme	intra-particle	1372:1385	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	53	theme	intra-particle	1372:1385	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	53	theme	intra-particle	1372:1385	arg1	diffusion					1387:1395	LDH intra-particle diffusion	1368:1395	LDH intra-particle diffusion	1368:1395	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	54	theme	concurrent	1406:1415	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	54	theme	concurrent	1406:1415	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	54	theme	concurrent	1406:1415	arg1	leaching					1417:1424	also concurrent leaching	1401:1424	also concurrent leaching of the FA anions from LDH structure	1401:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	4	55	theme	intercalated	617:628	arg1	bio-polymer					701:711	bio-polymer	701:711	bio-polymer	701:711	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	4	55	theme	intercalated	617:628	arg1	LDH-FA					635:640	FA intercalated LDH (LDH-FA)	614:641	FA intercalated LDH (LDH-FA)	614:641	Then, FA intercalated LDH (LDH-FA) was composited with different weights of chitosan (Cs) as bio-polymer and the obtained nanocomposite beads was characterized.
30393134	7	56	theme	anions	1436:1441	arg1	leaching					1417:1424	also concurrent leaching	1401:1424	also concurrent leaching of the FA anions from LDH structure	1401:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	56	theme	anions	1436:1441	arg1	processes					1267:1275	some release processes	1254:1275	some release processes such as diffusion controlled of flat surface by heterogeneous sites	1254:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	56	theme	anions	1436:1441	arg1	diffusion					1285:1293	diffusion	1285:1293	diffusion controlled of flat surface by heterogeneous sites	1285:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	56	theme	anions	1436:1441	arg1	process					1359:1365	ion exchange process	1346:1365	ion exchange process	1346:1365	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	56	theme	anions	1436:1441	arg1	diffusion					1387:1395	LDH intra-particle diffusion	1368:1395	LDH intra-particle diffusion	1368:1395	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	7	57	theme	heterogeneous	1325:1337	arg1	sites					1339:1343	heterogeneous sites	1325:1343	heterogeneous sites	1325:1343	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	6	58	dep	higher	1033:1038	arg1	shield					1040:1045	shield	1040:1045	shield	1040:1045	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	7	59	theme	well-known	1155:1164	arg1	models					1174:1179	several well-known kinetic models	1147:1179	several well-known kinetic models	1147:1179	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	5	60	theme	simulated	843:851	arg1	condition					853:861	simulated condition	843:861	simulated condition similar to the gastrointestinal tract with different pHs	843:918	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	6	61	theme	bio-composite	934:946	arg1	beads					948:952	The achieved bio-composite beads	921:952	The achieved bio-composite beads	921:952	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	6	62	theme	release	1068:1074	arg1	rate					1076:1079	the release rate	1064:1079	the release rate of FA drug	1064:1090	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	3	63	theme	exchange	576:583	arg1	methods					585:591	coprecipitation and anion exchange methods	550:591	coprecipitation and anion exchange methods	550:591	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	5	64	theme	similar	863:869	arg1	condition					853:861	simulated condition	843:861	simulated condition similar to the gastrointestinal tract with different pHs	843:918	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	6	65	dep	showed	954:959	arg1	showed					1022:1027	showed	1022:1027	showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH	954:1058	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	6	66	theme	achieved	925:932	arg1	beads					948:952	The achieved bio-composite beads	921:952	The achieved bio-composite beads	921:952	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	2	67	theme	drug	365:368	arg1	existence					349:357	The existence	345:357	The existence of FA drug between the LDH layers	345:391	The existence of FA drug between the LDH layers was confirmed with FT-IR and XRD analyses.
30393134	5	68	theme	different	906:914	arg1	pHs					916:918	different pHs	906:918	different pHs	906:918	Subsequence, the release of FA drug from obtained hybrids was examined at simulated condition similar to the gastrointestinal tract with different pHs.
30393134	6	69	theme	pH-sensitive	965:976	arg1	property					987:994	the pH-sensitive swelling property	961:994	the pH-sensitive swelling property and also the release test	961:1020	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	3	70	theme	UV-Vis	457:462	arg1	results					446:452	the results	442:452	the results of UV-Vis	442:462	Also, the results of UV-Vis confirmed that the FA loading in the structure of LDH was about 33.35% and 15.57% for coprecipitation and anion exchange methods, respectively.
30393134	7	71	theme	release	1220:1226	arg1	mechanism					1228:1236	the FA release mechanism	1213:1236	the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure	1213:1460	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30393134	0	72	theme	folic	82:86	arg1	acid					88:91	folic acid	82:91	folic acid	82:91	Fabrication and characterization of pH-sensitive bio-nanocomposite beads havening folic acid intercalated LDH and chitosan: Drug release and mechanism evaluation.
30393134	6	73	theme	acidic	1050:1055	arg1	pH					1057:1058	acidic pH	1050:1058	acidic pH	1050:1058	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	6	74	theme	drug	1087:1090	arg1	rate					1076:1079	the release rate	1064:1079	the release rate of FA drug	1064:1090	The achieved bio-composite beads showed the pH-sensitive swelling property and also the release test showed the higher shield of acidic pH and the release rate of FA drug was decreased with addition of Cs concentration.
30393134	7	75	theme	several	1147:1153	arg1	models					1174:1179	several well-known kinetic models	1147:1179	several well-known kinetic models	1147:1179	Also, several well-known kinetic models were examined in order to study the FA release mechanism which suggested some release processes such as diffusion controlled of flat surface by heterogeneous sites, ion exchange process, LDH intra-particle diffusion and also concurrent leaching of the FA anions from LDH structure.
30910055	0	0	theme	H1N1	102:105	arg1	virus					107:111	H1N1 virus	102:111	H1N1 virus infected mice	102:125	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	5	1	theme	goblet	642:647	arg1	cells					649:653	goblet cells	642:653	goblet cells	642:653	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	11	2	theme	high-value	1396:1405	arg1	application					1407:1417	the high-value application	1392:1417	the high-value application of HCP	1392:1424	This study proved the high-value application of HCP.
30910055	8	3	theme	Toll-like	1086:1094	arg1	receptors					1096:1104	Toll-like receptors	1086:1104	Toll-like receptors	1086:1104	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	2	4	from	microbiota	316:325	arg1	mice					350:353	H1N1 virus infected mice	330:353	H1N1 virus infected mice	330:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	5	5	from	intestine	704:712	arg1	level					673:677	the level	669:677	the level of zonula occludens-1 in intestine	669:712	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	0	6	theme	infected	113:120	arg1	mice					122:125	H1N1 virus infected mice	102:125	H1N1 virus infected mice	102:125	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	4	7	theme	pulmonary	473:481	arg1	injury					498:503	pulmonary and intestinal injury	473:503	pulmonary and intestinal injury	473:503	H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance.
30910055	9	8	theme	Oral	1194:1197	arg1	administration					1199:1212	Oral administration	1194:1212	Oral administration of HCP	1194:1219	Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection.
30910055	6	9	with	infection	796:804	arg1	abundances					843:852	significantly reduced relative abundances	812:852	significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	812:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	0	10	theme	virus	107:111	arg1	mice					122:125	H1N1 virus infected mice	102:125	H1N1 virus infected mice	102:125	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	6	11	theme	bacterial	893:901	arg1	genera					903:908	the pathogenic bacterial genera	878:908	the pathogenic bacterial genera	878:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	5	12	theme	inducible	590:598	arg1	factor-1α					600:608	hypoxia inducible factor-1α	582:608	hypoxia inducible factor-1α	582:608	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	2	13	theme	HCP	286:288	arg1	effects					275:281	the effects	271:281	the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice	271:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	10	14	theme	systemic	1310:1317	arg1	treatment					1319:1327	systemic treatment	1310:1327	systemic treatment	1310:1327	HCP may gain systemic treatment by local acting on intestine and microbiota.
30910055	5	15	from	occludens-1	689:699	arg1	intestine					704:712	intestine	704:712	intestine	704:712	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	8	16	theme	interleukin-10	1178:1191	arg1	production					1164:1173	the increased production	1150:1173	the increased production of interleukin-10	1150:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	8	16	theme	interleukin-10	1178:1191	arg1	intestine					1128:1136	intestine	1128:1136	intestine	1128:1136	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	2	17	theme	infected	341:348	arg1	mice					350:353	H1N1 virus infected mice	330:353	H1N1 virus infected mice	330:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	5	18	theme	factor-1α	600:608	arg1	expression					568:577	the expression	564:577	the expression of hypoxia inducible factor-1α	564:608	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	6	19	theme	Vibrio	857:862	arg1	abundances					843:852	significantly reduced relative abundances	812:852	significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	812:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	4	20	theme	H1N1	451:454	arg1	infection					456:464	H1N1 infection	451:464	H1N1 infection	451:464	H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance.
30910055	9	21	theme	HCP	1217:1219	arg1	administration					1199:1212	Oral administration	1194:1212	Oral administration of HCP	1194:1219	Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection.
30910055	1	22	theme	Houttuynia	186:195	arg1	cordata					197:203	Houttuynia cordata	186:203	Houttuynia cordata	186:203	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	22	theme	Houttuynia	186:195	arg1	medicine					232:239	a key traditional Chinese medicine	206:239	a key traditional Chinese medicine	206:239	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	0	23	theme	cordata	11:17	arg1	polysaccharide					19:32	Houttuynia cordata polysaccharide	0:32	Houttuynia cordata polysaccharide	0:32	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	7	24	theme	gut	943:945	arg1	microbiota					947:956	the gut microbiota	939:956	the gut microbiota	939:956	Furthermore, HCP rebalanced the gut microbiota and restored the intestinal homeostasis to some degree.
30910055	6	25	theme	pathogenic	882:891	arg1	genera					903:908	the pathogenic bacterial genera	878:908	the pathogenic bacterial genera	878:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	0	26	theme	Houttuynia	0:9	arg1	polysaccharide					19:32	Houttuynia cordata polysaccharide	0:32	Houttuynia cordata polysaccharide	0:32	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	5	27	from	mucosubstances	624:637	arg1	cells					649:653	goblet cells	642:653	goblet cells	642:653	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	8	28	theme	reduced	1069:1075	arg1	level					1077:1081	the reduced level	1065:1081	the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10	1065:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	6	29	theme	microbiota	770:779	arg1	change					749:754	the composition change	733:754	the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	733:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	11	30	theme	HCP	1422:1424	arg1	application					1407:1417	the high-value application	1392:1417	the high-value application of HCP	1392:1424	This study proved the high-value application of HCP.
30910055	8	31	theme	receptors	1096:1104	arg1	level					1077:1081	the reduced level	1065:1081	the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10	1065:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	4	32	theme	microbiota	513:522	arg1	imbalance					524:532	gut microbiota imbalance	509:532	gut microbiota imbalance	509:532	H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance.
30910055	5	33	theme	zonula	682:687	arg1	occludens-1					689:699	zonula occludens-1	682:699	zonula occludens-1 in intestine	682:712	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	6	34	theme	H1N1	791:794	arg1	infection					796:804	H1N1 infection	791:804	H1N1 infection	791:804	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	10	35	theme	local	1332:1336	arg1	acting					1338:1343	local acting	1332:1343	local acting on intestine and microbiota	1332:1371	HCP may gain systemic treatment by local acting on intestine and microbiota.
30910055	9	36	theme	lung	1232:1235	arg1	injury					1237:1242	lung injury	1232:1242	lung injury	1232:1242	Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection.
30910055	5	37	theme	occludens-1	689:699	arg1	level					673:677	the level	669:677	the level of zonula occludens-1 in intestine	669:712	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	2	38	theme	intestinal	293:302	arg1	barrier					304:310	intestinal barrier	293:310	intestinal barrier	293:310	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	1	39	theme	key	208:210	arg1	cordata					197:203	Houttuynia cordata	186:203	Houttuynia cordata	186:203	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	39	theme	key	208:210	arg1	medicine					232:239	a key traditional Chinese medicine	206:239	a key traditional Chinese medicine	206:239	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	3	40	theme	administrated	402:414	arg1	HCP					416:418	orally administrated HCP	395:418	orally administrated HCP	395:418	Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg(kg-1(d-1.
30910055	2	41	theme	virus	335:339	arg1	mice					350:353	H1N1 virus infected mice	330:353	H1N1 virus infected mice	330:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	9	42	theme	intestinal	1248:1257	arg1	dysfunction					1259:1269	intestinal dysfunction	1248:1269	intestinal dysfunction	1248:1269	Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection.
30910055	1	43	theme	traditional	212:222	arg1	cordata					197:203	Houttuynia cordata	186:203	Houttuynia cordata	186:203	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	43	theme	traditional	212:222	arg1	medicine					232:239	a key traditional Chinese medicine	206:239	a key traditional Chinese medicine	206:239	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	0	44	theme	intestinal	45:54	arg1	injury					56:61	intestinal injury	45:61	intestinal injury	45:61	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	8	45	from	level	1077:1081	arg1	production					1164:1173	the increased production	1150:1173	the increased production of interleukin-10	1150:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	8	45	from	level	1077:1081	arg1	intestine					1128:1136	intestine	1128:1136	intestine	1128:1136	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	6	46	dep	genera	903:908	arg1	Bacillus					868:875	Bacillus, the pathogenic bacterial genera	868:908	Bacillus	868:875	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	0	47	from	microbiota	88:97	arg1	mice					122:125	H1N1 virus infected mice	102:125	H1N1 virus infected mice	102:125	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	1	48	theme	Chinese	224:230	arg1	cordata					197:203	Houttuynia cordata	186:203	Houttuynia cordata	186:203	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	48	theme	Chinese	224:230	arg1	medicine					232:239	a key traditional Chinese medicine	206:239	a key traditional Chinese medicine	206:239	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	4	49	theme	intestinal	487:496	arg1	injury					498:503	pulmonary and intestinal injury	473:503	pulmonary and intestinal injury	473:503	H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance.
30910055	1	50	theme	Houttuynia	128:137	arg1	polysaccharide					147:160	Houttuynia cordata polysaccharide	128:160	Houttuynia cordata polysaccharide (HCP)	128:166	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	50	theme	Houttuynia	128:137	arg1	HCP					163:165	HCP	163:165	HCP	163:165	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	6	51	theme	genera	903:908	arg1	abundances					843:852	significantly reduced relative abundances	812:852	significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	812:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	4	52	theme	gut	509:511	arg1	imbalance					524:532	gut microbiota imbalance	509:532	gut microbiota imbalance	509:532	H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance.
30910055	8	53	theme	interleukin-1β	1110:1123	arg1	level					1077:1081	the reduced level	1065:1081	the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10	1065:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	8	54	theme	inflammation	1032:1043	arg1	inhibition					1018:1027	The inhibition	1014:1027	The inhibition of inflammation	1014:1043	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
30910055	9	55	theme	H1N1	1281:1284	arg1	infection					1286:1294	H1N1 infection	1281:1294	H1N1 infection	1281:1294	Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection.
30910055	6	56	theme	composition	737:747	arg1	change					749:754	the composition change	733:754	the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	733:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	1	57	theme	cordata	139:145	arg1	polysaccharide					147:160	Houttuynia cordata polysaccharide	128:160	Houttuynia cordata polysaccharide (HCP)	128:166	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	1	57	theme	cordata	139:145	arg1	HCP					163:165	HCP	163:165	HCP	163:165	Houttuynia cordata polysaccharide (HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine.
30910055	6	58	theme	intestinal	759:768	arg1	microbiota					770:779	intestinal microbiota	759:779	intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	759:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	6	59	theme	relative	834:841	arg1	abundances					843:852	significantly reduced relative abundances	812:852	significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	812:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	2	60	theme	H1N1	330:333	arg1	virus					335:339	H1N1 virus	330:339	H1N1 virus infected mice	330:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	2	61	from	effects	275:281	arg1	barrier					304:310	intestinal barrier	293:310	intestinal barrier	293:310	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	2	61	from	effects	275:281	arg1	microbiota					316:325	microbiota	316:325	microbiota	316:325	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	5	62	from	level	673:677	arg1	intestine					704:712	intestine	704:712	intestine	704:712	HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine.
30910055	6	63	theme	reduced	826:832	arg1	abundances					843:852	significantly reduced relative abundances	812:852	significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera	812:908	HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera.
30910055	3	64	dep	mg	438:439	arg1	d-1					446:448	d-1	446:448	40 mg(kg-1(d-1	435:448	Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg(kg-1(d-1.
30910055	3	64	dep	mg	438:439	arg1	kg-1					441:444	kg-1	441:444	40 mg(kg-1(d-1	435:448	Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg(kg-1(d-1.
30910055	3	65	theme	mg	438:439	arg1	dosage					425:430	a dosage	423:430	a dosage of 40 mg(kg-1(d-1	423:448	Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg(kg-1(d-1.
30910055	10	66	from	acting	1338:1343	arg1	microbiota					1362:1371	microbiota	1362:1371	microbiota	1362:1371	HCP may gain systemic treatment by local acting on intestine and microbiota.
30910055	10	66	from	acting	1338:1343	arg1	intestine					1348:1356	intestine	1348:1356	intestine	1348:1356	HCP may gain systemic treatment by local acting on intestine and microbiota.
30910055	0	67	theme	intestinal	77:86	arg1	microbiota					88:97	intestinal microbiota	77:97	intestinal microbiota in H1N1 virus infected mice	77:125	Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice.
30910055	7	68	theme	intestinal	975:984	arg1	homeostasis					986:996	the intestinal homeostasis	971:996	the intestinal homeostasis	971:996	Furthermore, HCP rebalanced the gut microbiota and restored the intestinal homeostasis to some degree.
30910055	3	69	theme	H1N1	380:383	arg1	virus					385:389	H1N1 virus	380:389	H1N1 virus	380:389	Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg(kg-1(d-1.
30910055	2	70	from	barrier	304:310	arg1	mice					350:353	H1N1 virus infected mice	330:353	H1N1 virus infected mice	330:353	The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice.
30910055	8	71	theme	increased	1154:1162	arg1	production					1164:1173	the increased production	1150:1173	the increased production of interleukin-10	1150:1191	The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10.
29691007	0	0	theme	clove	94:98	arg1	oil					110:112	clove essential oil	94:112	clove essential oil	94:112	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	5	1	theme	oil	688:690	arg1	amounts					661:667	Relative low amounts	648:667	Relative low amounts of clove essential oil	648:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	5	1	theme	oil	688:690	arg1	oil					688:690	clove essential oil	672:690	clove essential oil	672:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	1	2	from	20 wt	260:264	arg1	concentrations					205:218	various concentrations	197:218	various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt	197:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	4	3	theme	PDMS	516:519	arg1	content					521:527	the PDMS content	512:527	the PDMS content	512:527	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	4	from	number	450:455	arg1	composite					490:498	the composite	486:498	the composite	486:498	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	8	5	theme	composite	874:882	arg1	flexibility					826:836	the flexibility	822:836	the flexibility	822:836	The essential oil increased the flexibility and the antioxidant capacity of the composite.
29691007	8	5	theme	composite	874:882	arg1	capacity					858:865	the antioxidant capacity	842:865	the antioxidant capacity of the composite	842:882	The essential oil increased the flexibility and the antioxidant capacity of the composite.
29691007	5	6	theme	Relative	648:655	arg1	amounts					661:667	Relative low amounts	648:667	Relative low amounts of clove essential oil	648:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	5	6	theme	Relative	648:655	arg1	oil					688:690	clove essential oil	672:690	clove essential oil	672:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	7	7	theme	%	785:785	arg1	PDMS					787:790	% PDMS	785:790	% PDMS	785:790	% PDMS).
29691007	0	8	theme	essential	100:108	arg1	oil					110:112	clove essential oil	94:112	clove essential oil	94:112	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	4	9	theme	water	628:632	arg1	permeability					634:645	water permeability	628:645	water permeability	628:645	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	1	10	theme	composite	164:172	arg1	films					174:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	0	11	theme	RTV	43:45	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	5	12	theme	waterproof	720:729	arg1	80 wt					751:755	the most waterproof composite material (80 wt	711:755	the most waterproof composite material (80 wt	711:755	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	3	13	theme	PDMS-rich	363:371	arg1	particles					373:381	PDMS-rich particles	363:381	PDMS-rich particles	363:381	Morphological and chemical analysis by EDX-SEM and ATR-FTIR showed that EC-rich matrices and PDMS-rich particles were formed, with the two polymers interacting through Hbonds.
29691007	0	14	theme	Antimicrobial	0:12	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	10	15	theme	clove	1052:1056	arg1	oil					1068:1070	clove essential oil	1052:1070	clove essential oil	1052:1070	The presence of clove essential oil reduced the biofilm formation on the composites.
29691007	5	16	theme	composite	731:739	arg1	80 wt					751:755	the most waterproof composite material (80 wt	711:755	the most waterproof composite material (80 wt	711:755	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	10	17	from	formation	1092:1100	arg1	composites					1109:1118	the composites	1105:1118	the composites	1105:1118	The presence of clove essential oil reduced the biofilm formation on the composites.
29691007	4	18	theme	several	558:564	arg1	properties					566:575	several properties	558:575	several properties such as opacity, hydrophobicity, water uptake, and water permeability	558:645	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	18	theme	several	558:564	arg1	opacity					585:591	opacity	585:591	opacity	585:591	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	18	theme	several	558:564	arg1	permeability					634:645	water permeability	628:645	water permeability	628:645	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	18	theme	several	558:564	arg1	uptake					616:621	water uptake	610:621	water uptake	610:621	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	18	theme	several	558:564	arg1	hydrophobicity					594:607	hydrophobicity	594:607	hydrophobicity	594:607	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	0	19	theme	antioxidant	15:25	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	1	20	theme	Ethyl	115:119	arg1	PDMS					158:161	PDMS	158:161	PDMS	158:161	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	20	theme	Ethyl	115:119	arg1	/polydimethylsiloxane					135:155	Ethyl cellulose (EC)/polydimethylsiloxane	115:155	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	21	theme	various	197:203	arg1	concentrations					205:218	various concentrations	197:218	various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt	197:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	0	22	theme	waterproof	32:41	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	10	23	theme	biofilm	1084:1090	arg1	formation					1092:1100	the biofilm formation	1080:1100	the biofilm formation on the composites	1080:1118	The presence of clove essential oil reduced the biofilm formation on the composites.
29691007	1	24	dep	films	235:239	arg1	15					252:253	15	252:253	15	252:253	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	8	25	theme	essential	798:806	arg1	oil					808:810	The essential oil	794:810	The essential oil	794:810	The essential oil increased the flexibility and the antioxidant capacity of the composite.
29691007	4	26	theme	water	610:614	arg1	uptake					616:621	water uptake	610:621	water uptake	610:621	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	5	27	theme	low	657:659	arg1	amounts					661:667	Relative low amounts	648:667	Relative low amounts of clove essential oil	648:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	5	27	theme	low	657:659	arg1	oil					688:690	clove essential oil	672:690	clove essential oil	672:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	1	28	from	concentrations	205:218	arg1	films					235:239	the films	231:239	the films (0, 5, 10, 15	231:253	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	28	from	concentrations	205:218	arg1	20 wt					260:264	20 wt	260:264	20 wt	260:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	3	29	theme	chemical	288:295	arg1	analysis					297:304	Morphological and chemical analysis	270:304	Morphological and chemical analysis by EDX-SEM and ATR-FTIR	270:328	Morphological and chemical analysis by EDX-SEM and ATR-FTIR showed that EC-rich matrices and PDMS-rich particles were formed, with the two polymers interacting through Hbonds.
29691007	0	30	theme	silicone-ethyl	47:60	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	4	31	dep	number	450:455	arg1	The					446:448	The	446:448	The	446:448	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	32	from	diameter	461:468	arg1	composite					490:498	the composite	486:498	the composite	486:498	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	3	33	theme	Morphological	270:282	arg1	analysis					297:304	Morphological and chemical analysis	270:304	Morphological and chemical analysis by EDX-SEM and ATR-FTIR	270:328	Morphological and chemical analysis by EDX-SEM and ATR-FTIR showed that EC-rich matrices and PDMS-rich particles were formed, with the two polymers interacting through Hbonds.
29691007	1	34	theme	cellulose	121:129	arg1	PDMS					158:161	PDMS	158:161	PDMS	158:161	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	34	theme	cellulose	121:129	arg1	/polydimethylsiloxane					135:155	Ethyl cellulose (EC)/polydimethylsiloxane	115:155	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	3	35	theme	EC-rich	342:348	arg1	matrices					350:357	EC-rich matrices	342:357	EC-rich matrices	342:357	Morphological and chemical analysis by EDX-SEM and ATR-FTIR showed that EC-rich matrices and PDMS-rich particles were formed, with the two polymers interacting through Hbonds.
29691007	10	36	theme	oil	1068:1070	arg1	presence					1040:1047	The presence	1036:1047	The presence of clove essential oil	1036:1070	The presence of clove essential oil reduced the biofilm formation on the composites.
29691007	1	37	from	films	235:239	arg1	concentrations					205:218	various concentrations	197:218	various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt	197:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	4	38	theme	particles	473:481	arg1	diameter					461:468	diameter	461:468	diameter	461:468	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	4	38	theme	particles	473:481	arg1	number					450:455	number	450:455	number	450:455	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	1	39	from	PDMS	223:226	arg1	films					235:239	the films	231:239	the films (0, 5, 10, 15	231:253	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	39	from	PDMS	223:226	arg1	20 wt					260:264	20 wt	260:264	20 wt	260:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	5	40	theme	essential	678:686	arg1	oil					688:690	clove essential oil	672:690	clove essential oil	672:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	1	41	theme	EC	132:133	arg1	PDMS					158:161	PDMS	158:161	PDMS	158:161	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	41	theme	EC	132:133	arg1	/polydimethylsiloxane					135:155	Ethyl cellulose (EC)/polydimethylsiloxane	115:155	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	1	42	theme	PDMS	223:226	arg1	concentrations					205:218	various concentrations	197:218	various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt	197:264	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	8	43	theme	antioxidant	846:856	arg1	capacity					858:865	the antioxidant capacity	842:865	the antioxidant capacity of the composite	842:882	The essential oil increased the flexibility and the antioxidant capacity of the composite.
29691007	10	44	theme	essential	1058:1066	arg1	oil					1068:1070	clove essential oil	1052:1070	clove essential oil	1052:1070	The presence of clove essential oil reduced the biofilm formation on the composites.
29691007	5	45	theme	clove	672:676	arg1	oil					688:690	clove essential oil	672:690	clove essential oil	672:690	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	4	46	theme	properties	566:575	arg1	tuning					548:553	a fine tuning	541:553	a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability	541:645	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
29691007	1	47	theme	/polydimethylsiloxane	135:155	arg1	films					174:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films	115:178	Ethyl cellulose (EC)/polydimethylsiloxane (PDMS) composite films were prepared at various concentrations of PDMS in the films (0, 5, 10, 15, and 20 wt.
29691007	9	48	theme	common	943:948	arg1	coli					980:983	Escherichia coli	968:983	Escherichia coli	968:983	Finally, the antimicrobial properties were tested against common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
29691007	9	48	theme	common	943:948	arg1	aureus					1001:1006	Staphylococcus aureus	986:1006	Staphylococcus aureus	986:1006	Finally, the antimicrobial properties were tested against common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
29691007	9	48	theme	common	943:948	arg1	aeruginosa					1024:1033	Pseudomonas aeruginosa	1012:1033	Pseudomonas aeruginosa	1012:1033	Finally, the antimicrobial properties were tested against common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
29691007	9	48	theme	common	943:948	arg1	pathogens					950:958	common pathogens	943:958	common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa	943:1033	Finally, the antimicrobial properties were tested against common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
29691007	9	49	theme	antimicrobial	898:910	arg1	properties					912:921	the antimicrobial properties	894:921	the antimicrobial properties	894:921	Finally, the antimicrobial properties were tested against common pathogens such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.
29691007	0	50	theme	cellulose	62:70	arg1	composites					72:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites	0:81	Antimicrobial, antioxidant, and waterproof RTV silicone-ethyl cellulose composites containing clove essential oil.
29691007	5	51	theme	material	741:748	arg1	80 wt					751:755	the most waterproof composite material (80 wt	711:755	the most waterproof composite material (80 wt	711:755	Relative low amounts of clove essential oil were also added to the most waterproof composite material (80 wt.
29691007	4	52	theme	fine	543:546	arg1	tuning					548:553	a fine tuning	541:553	a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability	541:645	The number and diameter of particles in the composite depended on the PDMS content and allowed a fine tuning of several properties such as opacity, hydrophobicity, water uptake, and water permeability.
30889717	0	0	theme	diaphragmatic	77:89	arg1	CDH					99:101	CDH	99:101	CDH	99:101	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	0	0	theme	diaphragmatic	77:89	arg1	hernia					91:96	congenital diaphragmatic hernia	66:96	congenital diaphragmatic hernia (CDH)	66:102	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	9	1	theme	anatomical	1324:1333	arg1	shape					1335:1339	the anatomical shape	1320:1339	the anatomical shape	1320:1339	Furthermore, when injected in the tracheal model, good cohesion and capability to adapt to the anatomical shape were also observed.
30889717	2	2	theme	tracheal	328:335	arg1	FETO					348:351	FETO	348:351	FETO	348:351	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	2	2	theme	tracheal	328:335	arg1	occlusion					337:345	fetoscopic endoluminal tracheal occlusion	305:345	fetoscopic endoluminal tracheal occlusion (FETO)	305:352	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	10	3	theme	selected	1411:1418	arg1	compositions					1420:1431	selected compositions	1411:1431	selected compositions	1411:1431	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	1	4	theme	viscera	221:227	arg1	herniation					207:216	herniation	207:216	herniation of viscera in the thoracic cavity	207:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	4	theme	viscera	221:227	arg1	diaphragm					196:204	diaphragm	196:204	diaphragm	196:204	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	4	theme	viscera	221:227	arg1	development					270:280	impaired lung development	256:280	impaired lung development	256:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	0	5	theme	congenital	66:75	arg1	CDH					99:101	CDH	99:101	CDH	99:101	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	0	5	theme	congenital	66:75	arg1	hernia					91:96	congenital diaphragmatic hernia	66:96	congenital diaphragmatic hernia (CDH)	66:102	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	9	6	theme	good	1279:1282	arg1	cohesion					1284:1291	good cohesion	1279:1291	good cohesion	1279:1291	Furthermore, when injected in the tracheal model, good cohesion and capability to adapt to the anatomical shape were also observed.
30889717	13	7	theme	single-surgery	1826:1839	arg1	occlusion					1850:1858	single-surgery tracheal occlusion	1826:1858	single-surgery tracheal occlusion	1826:1858	However, due to their shear-thinning behaviour, HA-MC blends appeared as a particularly suitable candidate for single-surgery tracheal occlusion.
30889717	7	8	theme	mouse	1075:1079	arg1	fibroblasts					1081:1091	mouse fibroblasts	1075:1091	mouse fibroblasts	1075:1091	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	2	9	theme	endoluminal	316:326	arg1	FETO					348:351	FETO	348:351	FETO	348:351	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	2	9	theme	endoluminal	316:326	arg1	occlusion					337:345	fetoscopic endoluminal tracheal occlusion	305:345	fetoscopic endoluminal tracheal occlusion (FETO)	305:352	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	7	10	from	cytotoxicity	1046:1057	arg1	hydrogels					1096:1104	hydrogels	1096:1104	hydrogels	1096:1104	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	8	11	theme	adjusting	1150:1158	arg1	formulation					1160:1170	adjusting formulation	1150:1170	adjusting formulation	1150:1170	For both hydrogels, adjusting formulation enabled to obtain gelation time within the design range.
30889717	1	12	from	development	270:280	arg1	cavity					245:250	the thoracic cavity	232:250	the thoracic cavity	232:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	2	13	contain	contain	405:411	arg2	hypoplasia					423:432	pulmonary hypoplasia	413:432	pulmonary hypoplasia	413:432	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	2	13	contain	contain	405:411	arg1	FETO					348:351	FETO	348:351	FETO	348:351	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	2	13	contain	contain	405:411	arg1	occlusion					337:345	fetoscopic endoluminal tracheal occlusion	305:345	fetoscopic endoluminal tracheal occlusion (FETO)	305:352	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	13	14	theme	shear-thinning	1737:1750	arg1	behaviour					1752:1760	their shear-thinning behaviour	1731:1760	their shear-thinning behaviour	1731:1760	However, due to their shear-thinning behaviour, HA-MC blends appeared as a particularly suitable candidate for single-surgery tracheal occlusion.
30889717	6	15	theme	tracheal	885:892	arg1	casts					894:898	Anatomical tracheal casts	874:898	Anatomical tracheal casts	874:898	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	10	16	theme	sealing	1371:1377	arg1	up					1379:1380	Effective sealing up	1361:1380	Effective sealing up to 80 cm H20	1361:1393	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	1	17	theme	thoracic	236:243	arg1	cavity					245:250	the thoracic cavity	232:250	the thoracic cavity	232:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	4	18	theme	gels	653:656	arg1	types					644:648	Two different types	630:648	Two different types of gels	630:656	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	18	theme	gels	653:656	arg1	plugs					685:689	tracheal plugs	676:689	tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC)	676:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	6	19	theme	Anatomical	874:883	arg1	casts					894:898	Anatomical tracheal casts	874:898	Anatomical tracheal casts	874:898	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	7	20	theme	fibroblasts	1081:1091	arg1	cytotoxicity					1046:1057	In vitro cytotoxicity	1037:1057	In vitro cytotoxicity	1037:1057	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	7	20	theme	fibroblasts	1081:1091	arg1	adhesion					1063:1070	adhesion	1063:1070	adhesion of mouse fibroblasts on hydrogels	1063:1104	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	2	21	theme	catheter-mounted	377:392	arg1	balloon					394:400	a catheter-mounted balloon	375:400	a catheter-mounted balloon	375:400	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	4	22	theme	different	634:642	arg1	types					644:648	Two different types	630:648	Two different types of gels	630:656	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	22	theme	different	634:642	arg1	plugs					685:689	tracheal plugs	676:689	tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC)	676:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	7	23	from	adhesion	1063:1070	arg1	hydrogels					1096:1104	hydrogels	1096:1104	hydrogels	1096:1104	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	3	24	theme	associated	496:505	arg1	drawbacks					507:515	the associated drawbacks	492:515	the associated drawbacks	492:515	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	13	25	theme	tracheal	1841:1848	arg1	occlusion					1850:1858	single-surgery tracheal occlusion	1826:1858	single-surgery tracheal occlusion	1826:1858	However, due to their shear-thinning behaviour, HA-MC blends appeared as a particularly suitable candidate for single-surgery tracheal occlusion.
30889717	1	26	theme	birth	148:152	arg1	defect					154:159	a birth defect	146:159	a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	146:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	26	theme	birth	148:152	arg1	Hernia					130:135	Congenital Diaphragmatic Hernia	105:135	Congenital Diaphragmatic Hernia (CDH)	105:141	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	0	27	theme	injectable	3:12	arg1	hydrogel					26:33	An injectable, degradable hydrogel	0:33	An injectable, degradable hydrogel	0:33	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	6	28	theme	lung	944:947	arg1	fluid					949:953	a simulated lung fluid	932:953	a simulated lung fluid	932:953	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	1	29	from	cavity	245:250	arg1	closure					185:191	failed closure	178:191	failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	178:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	4	30	dep	plugs	685:689	arg1	HA-MC					748:752	HA-MC	748:752	HA-MC	748:752	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	30	dep	plugs	685:689	arg1	calcium-alginate					692:707	calcium-alginate	692:707	calcium-alginate	692:707	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	30	dep	plugs	685:689	arg1	blends					740:745	hyaluronan/methylcellulose blends	713:745	hyaluronan/methylcellulose blends (HA-MC)	713:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	30	dep	plugs	685:689	arg1	plugs					685:689	tracheal plugs	676:689	tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC)	676:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	12	31	theme	in	1628:1629	arg1	cytotoxicity					1637:1648	in vitro cytotoxicity	1628:1648	in vitro cytotoxicity	1628:1648	Despite the absence of in vitro cytotoxicity, a favourably low cell-adhesion was observed for both hydrogels.
30889717	3	32	dep	injectable	548:557	arg1	substitute					571:580	substitute	571:580	substitute	571:580	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	3	32	dep	injectable	548:557	arg1	degradable					560:569	degradable	560:569	degradable	560:569	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	2	33	theme	pulmonary	413:421	arg1	hypoplasia					423:432	pulmonary hypoplasia	413:432	pulmonary hypoplasia	413:432	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	4	34	theme	tracheal	676:683	arg1	types					644:648	Two different types	630:648	Two different types of gels	630:656	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	34	theme	tracheal	676:683	arg1	calcium-alginate					692:707	calcium-alginate	692:707	calcium-alginate	692:707	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	34	theme	tracheal	676:683	arg1	blends					740:745	hyaluronan/methylcellulose blends	713:745	hyaluronan/methylcellulose blends (HA-MC)	713:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	34	theme	tracheal	676:683	arg1	plugs					685:689	tracheal plugs	676:689	tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC)	676:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	5	35	theme	design	853:858	arg1	requirements					860:871	design requirements	853:871	design requirements	853:871	The effect of composition on hydrogel properties was evaluated, to identify formulations meeting design requirements.
30889717	5	36	theme	hydrogel	785:792	arg1	properties					794:803	hydrogel properties	785:803	hydrogel properties	785:803	The effect of composition on hydrogel properties was evaluated, to identify formulations meeting design requirements.
30889717	9	37	dep	cohesion	1284:1291	arg1	adapt					1311:1315	adapt	1311:1315	to adapt to the anatomical shape	1308:1339	Furthermore, when injected in the tracheal model, good cohesion and capability to adapt to the anatomical shape were also observed.
30889717	10	38	theme	in	1476:1477	arg1	model					1485:1489	the in vitro model	1472:1489	the in vitro model	1472:1489	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	10	39	theme	80 cm	1385:1389	arg1	H20					1391:1393	80 cm H20	1385:1393	80 cm H20	1385:1393	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	4	40	theme	hyaluronan/methylcellulose	713:738	arg1	HA-MC					748:752	HA-MC	748:752	HA-MC	748:752	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	40	theme	hyaluronan/methylcellulose	713:738	arg1	blends					740:745	hyaluronan/methylcellulose blends	713:745	hyaluronan/methylcellulose blends (HA-MC)	713:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	4	40	theme	hyaluronan/methylcellulose	713:738	arg1	plugs					685:689	tracheal plugs	676:689	tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC)	676:753	Two different types of gels were evaluated as tracheal plugs: calcium-alginate and hyaluronan/methylcellulose blends (HA-MC).
30889717	8	41	theme	gelation	1190:1197	arg1	time					1199:1202	gelation time	1190:1202	gelation time	1190:1202	For both hydrogels, adjusting formulation enabled to obtain gelation time within the design range.
30889717	1	42	theme	impaired	256:263	arg1	development					270:280	impaired lung development	256:280	impaired lung development	256:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	3	43	theme	FETO	466:469	arg1	rate					458:461	the success rate	446:461	the success rate of FETO	446:469	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	13	44	theme	suitable	1803:1810	arg1	candidate					1812:1820	a particularly suitable candidate	1788:1820	a particularly suitable candidate for single-surgery tracheal occlusion	1788:1858	However, due to their shear-thinning behaviour, HA-MC blends appeared as a particularly suitable candidate for single-surgery tracheal occlusion.
30889717	1	45	theme	failed	178:183	arg1	closure					185:191	failed closure	178:191	failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	178:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	46	theme	lung	265:268	arg1	development					270:280	impaired lung development	256:280	impaired lung development	256:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	3	47	theme	prenatal	612:619	arg1	removal					621:627	prenatal removal	612:627	prenatal removal	612:627	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	6	48	theme	plugs	1030:1034	arg1	cohesiveness					980:991	cohesiveness	980:991	cohesiveness	980:991	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	6	48	theme	plugs	1030:1034	arg1	pressure					1002:1009	sealing pressure	994:1009	sealing pressure	994:1009	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	6	48	theme	plugs	1030:1034	arg1	injectability					965:977	injectability	965:977	injectability	965:977	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	6	48	theme	plugs	1030:1034	arg1	persistence					1015:1025	persistence	1015:1025	persistence of plugs	1015:1034	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	1	49	theme	development	270:280	arg1	closure					185:191	failed closure	178:191	failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	178:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	11	50	theme	weight	1505:1510	arg1	loss					1512:1515	weight loss	1505:1515	weight loss	1505:1515	Swelling and weight loss were also strongly influenced by composition and varied from few days to several weeks.
30889717	1	51	from	closure	185:191	arg1	cavity					245:250	the thoracic cavity	232:250	the thoracic cavity	232:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	7	52	dep	In	1037:1038	arg1	vitro					1040:1044	vitro	1040:1044	vitro	1040:1044	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	0	53	theme	tracheal	44:51	arg1	occlusion					53:61	tracheal occlusion	44:61	tracheal occlusion in congenital diaphragmatic hernia (CDH)	44:102	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	6	54	theme	simulated	934:942	arg1	fluid					949:953	a simulated lung fluid	932:953	a simulated lung fluid	932:953	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
30889717	1	55	from	herniation	207:216	arg1	cavity					245:250	the thoracic cavity	232:250	the thoracic cavity	232:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	0	56	from	occlusion	53:61	arg1	CDH					99:101	CDH	99:101	CDH	99:101	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	0	56	from	occlusion	53:61	arg1	hernia					91:96	congenital diaphragmatic hernia	66:96	congenital diaphragmatic hernia (CDH)	66:102	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	2	57	theme	fetoscopic	305:314	arg1	FETO					348:351	FETO	348:351	FETO	348:351	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	2	57	theme	fetoscopic	305:314	arg1	occlusion					337:345	fetoscopic endoluminal tracheal occlusion	305:345	fetoscopic endoluminal tracheal occlusion (FETO)	305:352	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	8	58	theme	design	1215:1220	arg1	range					1222:1226	the design range	1211:1226	the design range	1211:1226	For both hydrogels, adjusting formulation enabled to obtain gelation time within the design range.
30889717	7	59	theme	In	1037:1038	arg1	cytotoxicity					1046:1057	In vitro cytotoxicity	1037:1057	In vitro cytotoxicity	1037:1057	In vitro cytotoxicity and adhesion of mouse fibroblasts on hydrogels were also investigated.
30889717	12	60	theme	cytotoxicity	1637:1648	arg1	absence					1617:1623	the absence	1613:1623	the absence of in vitro cytotoxicity	1613:1648	Despite the absence of in vitro cytotoxicity, a favourably low cell-adhesion was observed for both hydrogels.
30889717	1	61	theme	Congenital	105:114	arg1	defect					154:159	a birth defect	146:159	a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	146:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	61	theme	Congenital	105:114	arg1	CDH					138:140	CDH	138:140	CDH	138:140	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	61	theme	Congenital	105:114	arg1	Hernia					130:135	Congenital Diaphragmatic Hernia	105:135	Congenital Diaphragmatic Hernia (CDH)	105:141	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	10	62	theme	Effective	1361:1369	arg1	up					1379:1380	Effective sealing up	1361:1380	Effective sealing up to 80 cm H20	1361:1393	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	1	63	from	diaphragm	196:204	arg1	cavity					245:250	the thoracic cavity	232:250	the thoracic cavity	232:250	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	64	theme	Diaphragmatic	116:128	arg1	defect					154:159	a birth defect	146:159	a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	146:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	64	theme	Diaphragmatic	116:128	arg1	CDH					138:140	CDH	138:140	CDH	138:140	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	1	64	theme	Diaphragmatic	116:128	arg1	Hernia					130:135	Congenital Diaphragmatic Hernia	105:135	Congenital Diaphragmatic Hernia (CDH)	105:141	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	11	65	theme	few	1578:1580	arg1	days					1582:1585	few days	1578:1585	few days	1578:1585	Swelling and weight loss were also strongly influenced by composition and varied from few days to several weeks.
30889717	1	66	theme	diaphragm	196:204	arg1	closure					185:191	failed closure	178:191	failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	178:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	12	67	dep	in	1628:1629	arg1	vitro					1631:1635	vitro	1631:1635	vitro	1631:1635	Despite the absence of in vitro cytotoxicity, a favourably low cell-adhesion was observed for both hydrogels.
30889717	2	68	theme	severe	291:296	arg1	cases					298:302	most severe cases	286:302	most severe cases	286:302	In most severe cases, fetoscopic endoluminal tracheal occlusion (FETO) is performed through a catheter-mounted balloon to contain pulmonary hypoplasia.
30889717	3	69	theme	success	450:456	arg1	rate					458:461	the success rate	446:461	the success rate of FETO	446:469	To support the success rate of FETO and overcome some of the associated drawbacks, this work aimed at finding an injectable, degradable substitute for the balloon not requiring prenatal removal.
30889717	13	70	theme	HA-MC	1763:1767	arg1	blends					1769:1774	HA-MC blends	1763:1774	HA-MC blends	1763:1774	However, due to their shear-thinning behaviour, HA-MC blends appeared as a particularly suitable candidate for single-surgery tracheal occlusion.
30889717	9	71	theme	tracheal	1263:1270	arg1	model					1272:1276	the tracheal model	1259:1276	the tracheal model	1259:1276	Furthermore, when injected in the tracheal model, good cohesion and capability to adapt to the anatomical shape were also observed.
30889717	0	72	dep	injectable	3:12	arg1	degradable					15:24	degradable	15:24	degradable	15:24	An injectable, degradable hydrogel plug for tracheal occlusion in congenital diaphragmatic hernia (CDH).
30889717	11	73	theme	several	1590:1596	arg1	weeks					1598:1602	several weeks	1590:1602	several weeks	1590:1602	Swelling and weight loss were also strongly influenced by composition and varied from few days to several weeks.
30889717	12	74	theme	low	1664:1666	arg1	cell-adhesion					1668:1680	a favourably low cell-adhesion	1651:1680	a favourably low cell-adhesion	1651:1680	Despite the absence of in vitro cytotoxicity, a favourably low cell-adhesion was observed for both hydrogels.
30889717	1	75	theme	herniation	207:216	arg1	closure					185:191	failed closure	178:191	failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development	178:280	Congenital Diaphragmatic Hernia (CDH) is a birth defect characterized by failed closure of diaphragm, herniation of viscera in the thoracic cavity and impaired lung development.
30889717	5	76	from	effect	760:765	arg1	properties					794:803	hydrogel properties	785:803	hydrogel properties	785:803	The effect of composition on hydrogel properties was evaluated, to identify formulations meeting design requirements.
30889717	10	77	dep	in	1476:1477	arg1	vitro					1479:1483	vitro	1479:1483	vitro	1479:1483	Effective sealing up to 80 cm H20 was reached for selected compositions and was maintained for over 4 weeks in the in vitro model.
30889717	5	78	theme	composition	770:780	arg1	effect					760:765	The effect	756:765	The effect of composition on hydrogel properties	756:803	The effect of composition on hydrogel properties was evaluated, to identify formulations meeting design requirements.
30889717	6	79	theme	sealing	994:1000	arg1	pressure					1002:1009	sealing pressure	994:1009	sealing pressure	994:1009	Anatomical tracheal casts were fabricated and filled with a simulated lung fluid to assess injectability, cohesiveness, sealing pressure and persistence of plugs.
31221341	8	0	theme	-linked-mannopyranosyl	1234:1255	arg1	residues					1291:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	0	1	theme	Schizophyllum	86:98	arg1	commune					100:106	Schizophyllum commune	86:106	Schizophyllum commune	86:106	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	8	2	with	backbone	1152:1159	arg1	activity					1199:1206	excellent immune-enhancing activity	1172:1206	excellent immune-enhancing activity	1172:1206	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	6	3	with	treatment	1026:1034	arg1	polysaccharides					1047:1061	these polysaccharides	1041:1061	these polysaccharides	1041:1061	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	4	theme	kinase	1006:1011	arg1	levels					918:923	mRNA expression levels	902:923	mRNA expression levels	902:923	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	4	theme	kinase	1006:1011	arg1	activation					933:942	the activation	929:942	the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK)	929:1018	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	5	theme	expression	907:916	arg1	levels					918:923	mRNA expression levels	902:923	mRNA expression levels	902:923	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	0	6	from	Studies	0:6	arg1	properties					22:31	structural properties	11:31	structural properties	11:31	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	0	6	from	Studies	0:6	arg1	activities					54:63	immune-enhancing activities	37:63	immune-enhancing activities	37:63	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	3	7	theme	small	475:479	arg1	mannose					492:498	mannose	492:498	mannose	492:498	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	7	theme	small	475:479	arg1	amounts					481:487	small amounts	475:487	small amounts of mannose, galactose and xylose	475:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	7	theme	small	475:479	arg1	xylose					515:520	xylose	515:520	xylose	515:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	7	theme	small	475:479	arg1	galactose					501:509	galactose	501:509	galactose	501:509	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	4	8	theme	immune-enhancing	635:650	arg1	activities					652:661	Their immune-enhancing activities	629:661	Their immune-enhancing activities	629:661	Their immune-enhancing activities were tested using RAW264.7 cells.
31221341	6	9	theme	mRNA	902:905	arg1	levels					918:923	mRNA expression levels	902:923	mRNA expression levels	902:923	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	3	10	dep	glucose	444:450	arg1	%					457:457	75.5%	453:457	75.5%	453:457	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	10	dep	glucose	444:450	arg1	%					467:467	88.2%	463:467	88.2%	463:467	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	8	11	theme	excellent	1172:1180	arg1	activity					1199:1206	excellent immune-enhancing activity	1172:1206	excellent immune-enhancing activity	1172:1206	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	3	12	theme	xylose	621:626	arg1	galactose					597:605	galactose	597:605	galactose	597:605	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	12	theme	xylose	621:626	arg1	amounts					586:592	minor amounts	580:592	minor amounts of galactose, glucose, and xylose	580:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	12	theme	xylose	621:626	arg1	glucose					608:614	glucose	608:614	glucose	608:614	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	12	theme	xylose	621:626	arg1	xylose					621:626	xylose	621:626	xylose	621:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	6	13	theme	protein	998:1004	arg1	MAPK					1014:1017	MAPK	1014:1017	MAPK	1014:1017	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	13	theme	protein	998:1004	arg1	kinase					1006:1011	mitogen-activated protein kinase	980:1011	mitogen-activated protein kinase (MAPK)	980:1018	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	4	14	theme	RAW264.7	681:688	arg1	cells					690:694	RAW264.7 cells	681:694	RAW264.7 cells	681:694	Their immune-enhancing activities were tested using RAW264.7 cells.
31221341	8	15	theme	F2	1164:1165	arg1	backbone					1152:1159	The backbone	1148:1159	The backbone of F2 with excellent immune-enhancing activity	1148:1206	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	3	16	theme	Cr	410:411	arg1	compositions					394:405	Monosaccharide compositions	379:405	Monosaccharide compositions of Cr and F1	379:418	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	17	theme	glucose	608:614	arg1	galactose					597:605	galactose	597:605	galactose	597:605	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	17	theme	glucose	608:614	arg1	amounts					586:592	minor amounts	580:592	minor amounts of galactose, glucose, and xylose	580:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	17	theme	glucose	608:614	arg1	glucose					608:614	glucose	608:614	glucose	608:614	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	17	theme	glucose	608:614	arg1	xylose					621:626	xylose	621:626	xylose	621:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	18	with	glucose	444:450	arg1	mannose					492:498	mannose	492:498	mannose	492:498	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	18	with	glucose	444:450	arg1	amounts					481:487	small amounts	475:487	small amounts of mannose, galactose and xylose	475:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	18	with	glucose	444:450	arg1	xylose					515:520	xylose	515:520	xylose	515:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	18	with	glucose	444:450	arg1	galactose					501:509	galactose	501:509	galactose	501:509	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	6	19	theme	large	833:837	arg1	amounts					839:845	large amounts	833:845	large amounts of nitric oxide and various cytokines	833:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	19	theme	large	833:837	arg1	oxide					857:861	nitric oxide	850:861	nitric oxide	850:861	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	19	theme	large	833:837	arg1	cytokines					875:883	various cytokines	867:883	various cytokines	867:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	0	20	from	activities	54:63	arg1	commune					100:106	Schizophyllum commune	86:106	Schizophyllum commune	86:106	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	1	21	theme	structural	197:206	arg1	properties					229:238	their structural and immune-enhancing properties	191:238	their structural and immune-enhancing properties	191:238	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
31221341	5	22	theme	RAW264.7	723:730	arg1	cells					732:736	RAW264.7 cells	723:736	RAW264.7 cells	723:736	Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides.
31221341	3	23	theme	galactose	501:509	arg1	mannose					492:498	mannose	492:498	mannose	492:498	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	23	theme	galactose	501:509	arg1	amounts					481:487	small amounts	475:487	small amounts of mannose, galactose and xylose	475:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	23	theme	galactose	501:509	arg1	xylose					515:520	xylose	515:520	xylose	515:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	23	theme	galactose	501:509	arg1	galactose					501:509	galactose	501:509	galactose	501:509	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	5	24	theme	cells	732:736	arg1	activity					711:718	Proliferation activity	697:718	Proliferation activity of RAW264.7 cells	697:736	Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides.
31221341	6	25	theme	cytokines	875:883	arg1	amounts					839:845	large amounts	833:845	large amounts of nitric oxide and various cytokines	833:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	25	theme	cytokines	875:883	arg1	oxide					857:861	nitric oxide	850:861	nitric oxide	850:861	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	25	theme	cytokines	875:883	arg1	cytokines					875:883	various cytokines	867:883	various cytokines	867:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	3	26	with	manose	560:565	arg1	galactose					597:605	galactose	597:605	galactose	597:605	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	26	with	manose	560:565	arg1	amounts					586:592	minor amounts	580:592	minor amounts of galactose, glucose, and xylose	580:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	26	with	manose	560:565	arg1	glucose					608:614	glucose	608:614	glucose	608:614	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	26	with	manose	560:565	arg1	xylose					621:626	xylose	621:626	xylose	621:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	2	27	dep	Crude	259:263	arg1	F1					280:281	F1	280:281	F1	280:281	Crude and fractions (F1 and F2) were composed of sugars (50.3-82.8%), proteins (1.46-20.1%), and sulfates (1.33-7.01%).
31221341	2	27	dep	Crude	259:263	arg1	F2					287:288	F2	287:288	F2	287:288	Crude and fractions (F1 and F2) were composed of sugars (50.3-82.8%), proteins (1.46-20.1%), and sulfates (1.33-7.01%).
31221341	0	28	theme	structural	11:20	arg1	properties					22:31	structural properties	11:31	structural properties	11:31	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	6	29	theme	various	867:873	arg1	cytokines					875:883	various cytokines	867:883	various cytokines	867:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	0	30	from	commune	100:106	arg1	glycomannans					68:79	glycomannans	68:79	glycomannans from Schizophyllum commune	68:106	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	0	30	from	commune	100:106	arg1	properties					22:31	structural properties	11:31	structural properties	11:31	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	0	30	from	commune	100:106	arg1	activities					54:63	immune-enhancing activities	37:63	immune-enhancing activities	37:63	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	3	31	theme	mannose	492:498	arg1	mannose					492:498	mannose	492:498	mannose	492:498	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	31	theme	mannose	492:498	arg1	amounts					481:487	small amounts	475:487	small amounts of mannose, galactose and xylose	475:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	31	theme	mannose	492:498	arg1	xylose					515:520	xylose	515:520	xylose	515:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	31	theme	mannose	492:498	arg1	galactose					501:509	galactose	501:509	galactose	501:509	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	0	32	theme	immune-enhancing	37:52	arg1	activities					54:63	immune-enhancing activities	37:63	immune-enhancing activities	37:63	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	8	33	attach	linked	1219:1224	arg2	backbone					1152:1159	The backbone	1148:1159	The backbone of F2 with excellent immune-enhancing activity	1148:1206	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	8	33	attach	linked	1219:1224	arg3	residues					1291:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	6	34	theme	factor-kappa	955:966	arg1	NF-κB					969:973	nuclear factor-kappa (NF-κB)	947:974	nuclear factor-kappa (NF-κB)	947:974	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	3	35	theme	F1	417:418	arg1	compositions					394:405	Monosaccharide compositions	379:405	Monosaccharide compositions of Cr and F1	379:418	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	36	theme	Monosaccharide	379:392	arg1	compositions					394:405	Monosaccharide compositions	379:405	Monosaccharide compositions of Cr and F1	379:418	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	6	37	theme	oxide	857:861	arg1	amounts					839:845	large amounts	833:845	large amounts of nitric oxide and various cytokines	833:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	37	theme	oxide	857:861	arg1	oxide					857:861	nitric oxide	850:861	nitric oxide	850:861	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	37	theme	oxide	857:861	arg1	cytokines					875:883	various cytokines	867:883	various cytokines	867:883	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	38	theme	nuclear	947:953	arg1	NF-κB					969:973	nuclear factor-kappa (NF-κB)	947:974	nuclear factor-kappa (NF-κB)	947:974	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	5	39	theme	Proliferation	697:709	arg1	activity					711:718	Proliferation activity	697:718	Proliferation activity of RAW264.7 cells	697:736	Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides.
31221341	6	40	theme	nitric	850:855	arg1	oxide					857:861	nitric oxide	850:861	nitric oxide	850:861	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	8	41	theme	-linked-mannopyranosyl	1268:1289	arg1	residues					1291:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	(1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues	1229:1298	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	6	42	theme	RAW264.7	809:816	arg1	cells					818:822	RAW264.7 cells	809:822	RAW264.7 cells	809:822	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	0	43	from	properties	22:31	arg1	commune					100:106	Schizophyllum commune	86:106	Schizophyllum commune	86:106	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	3	44	theme	xylose	515:520	arg1	mannose					492:498	mannose	492:498	mannose	492:498	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	44	theme	xylose	515:520	arg1	amounts					481:487	small amounts	475:487	small amounts of mannose, galactose and xylose	475:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	44	theme	xylose	515:520	arg1	xylose					515:520	xylose	515:520	xylose	515:520	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	44	theme	xylose	515:520	arg1	galactose					501:509	galactose	501:509	galactose	501:509	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	1	45	theme	Schizophyllum	145:157	arg1	commune					159:165	Schizophyllum commune	145:165	Schizophyllum commune (a common mushroom)	145:185	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
31221341	1	45	theme	Schizophyllum	145:157	arg1	mushroom					177:184	a common mushroom	168:184	a common mushroom	168:184	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
31221341	5	46	with	treatment	758:766	arg1	polysaccharides					779:793	these polysaccharides	773:793	these polysaccharides	773:793	Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides.
31221341	3	47	theme	minor	580:584	arg1	galactose					597:605	galactose	597:605	galactose	597:605	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	47	theme	minor	580:584	arg1	amounts					586:592	minor amounts	580:592	minor amounts of galactose, glucose, and xylose	580:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	47	theme	minor	580:584	arg1	glucose					608:614	glucose	608:614	glucose	608:614	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	47	theme	minor	580:584	arg1	xylose					621:626	xylose	621:626	xylose	621:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	7	48	dep	CR-3	1122:1125	arg1	receptors					1137:1145	receptors	1137:1145	receptors	1137:1145	In addition, RAW264.7 cells were activated mainly through CR-3 and TLR-4 receptors.
31221341	6	49	theme	NF-κB	969:973	arg1	levels					918:923	mRNA expression levels	902:923	mRNA expression levels	902:923	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	49	theme	NF-κB	969:973	arg1	activation					933:942	the activation	929:942	the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK)	929:1018	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	8	50	theme	immune-enhancing	1182:1197	arg1	activity					1199:1206	excellent immune-enhancing activity	1172:1206	excellent immune-enhancing activity	1172:1206	The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
31221341	1	51	theme	immune-enhancing	212:227	arg1	properties					229:238	their structural and immune-enhancing properties	191:238	their structural and immune-enhancing properties	191:238	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
31221341	6	52	theme	mitogen-activated	980:996	arg1	MAPK					1014:1017	MAPK	1014:1017	MAPK	1014:1017	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	6	52	theme	mitogen-activated	980:996	arg1	kinase					1006:1011	mitogen-activated protein kinase	980:1011	mitogen-activated protein kinase (MAPK)	980:1018	In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides.
31221341	0	53	theme	glycomannans	68:79	arg1	properties					22:31	structural properties	11:31	structural properties	11:31	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	0	53	theme	glycomannans	68:79	arg1	activities					54:63	immune-enhancing activities	37:63	immune-enhancing activities	37:63	Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune.
31221341	3	54	theme	galactose	597:605	arg1	galactose					597:605	galactose	597:605	galactose	597:605	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	54	theme	galactose	597:605	arg1	amounts					586:592	minor amounts	580:592	minor amounts of galactose, glucose, and xylose	580:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	54	theme	galactose	597:605	arg1	glucose					608:614	glucose	608:614	glucose	608:614	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	3	54	theme	galactose	597:605	arg1	xylose					621:626	xylose	621:626	xylose	621:626	Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose.
31221341	7	55	theme	RAW264.7	1077:1084	arg1	cells					1086:1090	RAW264.7 cells	1077:1090	RAW264.7 cells	1077:1090	In addition, RAW264.7 cells were activated mainly through CR-3 and TLR-4 receptors.
31221341	1	56	theme	common	170:175	arg1	commune					159:165	Schizophyllum commune	145:165	Schizophyllum commune (a common mushroom)	145:185	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
31221341	1	56	theme	common	170:175	arg1	mushroom					177:184	a common mushroom	168:184	a common mushroom	168:184	Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated.
30900851	7	0	theme	significant	921:931	arg1	potential					933:941	significant potential	921:941	significant potential	921:941	The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
30900851	4	1	theme	positive	607:614	arg1	electricity					616:626	positive electricity	607:626	positive electricity	607:626	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	1	theme	positive	607:614	arg1	separation					579:588	rapid magnetic separation	564:588	rapid magnetic separation	564:588	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	6	2	theme	detection	838:846	arg1	fmol					874:877	0.02 fmol	869:877	0.02 fmol	869:877	After the enrichment step, the limit of detection was determined to be 0.02 fmol.
30900851	6	2	theme	detection	838:846	arg1	limit					829:833	the limit	825:833	the limit of detection	825:846	After the enrichment step, the limit of detection was determined to be 0.02 fmol.
30900851	7	3	theme	prepared	884:891	arg1	MNPs					903:906	The prepared POM-based MNPs	880:906	The prepared POM-based MNPs	880:906	The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
30900851	5	4	theme	enrichment	776:785	arg1	technique					787:795	the enrichment technique	772:795	the enrichment technique	772:795	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	2	5	used	used	268:271	arg2	technique					254:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan.
30900851	3	6	theme	ionization	465:474	arg1	time					476:479	matrix assisted laser desorption ionization time	432:479	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	5	7	theme	technique	787:795	arg1	performance					757:767	the performance	753:767	the performance of the enrichment technique	753:795	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	7	8	theme	phosphoproteins	977:991	arg1	detection					950:958	the detection	946:958	the detection of low-abundance phosphoproteins	946:991	The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
30900851	2	9	theme	magnetic	284:291	arg1	MNPs					308:311	MNPs	308:311	MNPs	308:311	A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan.
30900851	2	9	theme	magnetic	284:291	arg1	nanoparticles					293:305	magnetic nanoparticles	284:305	magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan	284:353	A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan.
30900851	3	10	theme	prior	411:415	arg1	enrichment					400:409	phosphopeptide enrichment	385:409	phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	385:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	11	theme	laser	448:452	arg1	ionization					465:474	matrix assisted laser desorption ionization	432:474	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	12	used	used	376:379	arg2	composites					360:369	The composites	356:369	The composites	356:369	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	13	theme	desorption	454:463	arg1	ionization					465:474	matrix assisted laser desorption ionization	432:474	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	14	theme	phosphopeptide	385:398	arg1	enrichment					400:409	phosphopeptide enrichment	385:409	phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	385:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	4	15	theme	separation	579:588	arg1	advantages					550:559	advantages	550:559	advantages of rapid magnetic separation, hydrophilicity, positive electricity,	550:627	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	15	theme	separation	579:588	arg1	selectivity					640:650	a high selectivity	633:650	a high selectivity for enrichment of phosphopeptides	633:684	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	7	16	theme	low-abundance	963:975	arg1	phosphoproteins					977:991	low-abundance phosphoproteins	963:991	low-abundance phosphoproteins	963:991	The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
30900851	3	17	theme	mass	491:494	arg1	MS					520:521	MALDI-TOF MS	510:521	MALDI-TOF MS	510:521	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	17	theme	mass	491:494	arg1	spectrometry					496:507	flight mass spectrometry	484:507	flight mass spectrometry (MALDI-TOF MS)	484:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	0	18	theme	polyoxometalate-chitosan	16:39	arg1	composite					50:58	polyoxometalate-chitosan magnetic composite	16:58	polyoxometalate-chitosan magnetic composite	16:58	[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides].
30900851	3	19	theme	spectrometry	496:507	arg1	time					476:479	matrix assisted laser desorption ionization time	432:479	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	20	theme	matrix	432:437	arg1	ionization					465:474	matrix assisted laser desorption ionization	432:474	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	7	21	theme	POM-based	893:901	arg1	MNPs					903:906	The prepared POM-based MNPs	880:906	The prepared POM-based MNPs	880:906	The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
30900851	0	22	theme	composite	50:58	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides	0:96	[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides].
30900851	3	23	theme	assisted	439:446	arg1	ionization					465:474	matrix assisted laser desorption ionization	432:474	matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)	432:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	2	24	theme	assembly	245:252	arg1	technique					254:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan.
30900851	6	25	theme	enrichment	808:817	arg1	step					819:822	the enrichment step	804:822	the enrichment step	804:822	After the enrichment step, the limit of detection was determined to be 0.02 fmol.
30900851	3	26	theme	flight	484:489	arg1	MS					520:521	MALDI-TOF MS	510:521	MALDI-TOF MS	510:521	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	26	theme	flight	484:489	arg1	spectrometry					496:507	flight mass spectrometry	484:507	flight mass spectrometry (MALDI-TOF MS)	484:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	2	27	theme	layer-by-layer	230:243	arg1	technique					254:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique	228:262	A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan.
30900851	4	28	theme	magnetic	570:577	arg1	hydrophilicity					591:604	hydrophilicity	591:604	hydrophilicity	591:604	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	28	theme	magnetic	570:577	arg1	electricity					616:626	positive electricity	607:626	positive electricity	607:626	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	28	theme	magnetic	570:577	arg1	separation					579:588	rapid magnetic separation	564:588	rapid magnetic separation	564:588	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	5	29	theme	model	714:718	arg1	protein					735:741	a model phosphorylated protein	712:741	a model phosphorylated protein	712:741	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	5	29	theme	model	714:718	arg1	β-Casein					687:694	β-Casein	687:694	β-Casein	687:694	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	4	30	theme	rapid	564:568	arg1	hydrophilicity					591:604	hydrophilicity	591:604	hydrophilicity	591:604	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	30	theme	rapid	564:568	arg1	electricity					616:626	positive electricity	607:626	positive electricity	607:626	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	4	30	theme	rapid	564:568	arg1	separation					579:588	rapid magnetic separation	564:588	rapid magnetic separation	564:588	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	5	31	theme	phosphorylated	720:733	arg1	protein					735:741	a model phosphorylated protein	712:741	a model phosphorylated protein	712:741	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	5	31	theme	phosphorylated	720:733	arg1	β-Casein					687:694	β-Casein	687:694	β-Casein	687:694	β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique.
30900851	1	32	theme	phosphopeptides	131:145	arg1	enrichment					117:126	the enrichment	113:126	the enrichment of phosphopeptides	113:145	A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate (POM) was developed.
30900851	4	33	theme	high	635:638	arg1	selectivity					640:650	a high selectivity	633:650	a high selectivity for enrichment of phosphopeptides	633:684	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	3	34	theme	MALDI-TOF	510:518	arg1	MS					520:521	MALDI-TOF MS	510:521	MALDI-TOF MS	510:521	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	3	34	theme	MALDI-TOF	510:518	arg1	spectrometry					496:507	flight mass spectrometry	484:507	flight mass spectrometry (MALDI-TOF MS)	484:522	The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
30900851	0	35	theme	phosphopeptides	82:96	arg1	enrichment					68:77	the enrichment	64:77	the enrichment of phosphopeptides	64:96	[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides].
30900851	4	36	theme	phosphopeptides	670:684	arg1	enrichment					656:665	enrichment	656:665	enrichment of phosphopeptides	656:684	The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides.
30900851	0	37	theme	magnetic	41:48	arg1	composite					50:58	polyoxometalate-chitosan magnetic composite	16:58	polyoxometalate-chitosan magnetic composite	16:58	[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides].
30900851	1	38	theme	magnetic	156:163	arg1	nanoparticles					165:177	magnetic nanoparticles	156:177	magnetic nanoparticles coated with polyoxometalate (POM)	156:211	A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate (POM) was developed.
30690237	0	0	theme	zerovalent	68:77	arg1	composite					104:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	4	1	theme	kinetics	915:922	arg1	analysis					883:890	the XPS analysis and fitting results	875:910	analysis	883:890	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	1	theme	kinetics	915:922	arg1	results					904:910	the XPS analysis and fitting results	875:910	results	904:910	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	6	2	theme	new	1255:1257	arg1	insights					1259:1266	new insights	1255:1266	new insights into the design of high-performance engineered biochar for wastewater treatment	1255:1346	These results provide new insights into the design of high-performance engineered biochar for wastewater treatment.
30690237	4	3	theme	Redlich-Peterson	961:976	arg1	isotherms					950:958	isotherms	950:958	isotherms (Redlich-Peterson model)	950:983	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	3	theme	Redlich-Peterson	961:976	arg1	model					978:982	Redlich-Peterson model	961:982	Redlich-Peterson model	961:982	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	0	4	theme	nanoscale	58:66	arg1	composite					104:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	5	5	theme	column	1213:1218	arg1	experiments					1220:1230	the fixed-bed column experiments	1199:1230	the fixed-bed column experiments	1199:1230	The practical applicability of nZVI/BC/CA composites was further demonstrated through the fixed-bed column experiments.
30690237	1	6	theme	nanoscale	254:262	arg1	reactions					297:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	2	7	theme	spherical	527:535	arg1	shape					537:541	a spherical shape	525:541	a spherical shape	525:541	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	4	8	theme	micro-electrolysis	1058:1075	arg1	reactions					1077:1085	micro-electrolysis reactions	1058:1085	micro-electrolysis reactions	1058:1085	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	5	9	theme	fixed-bed	1203:1211	arg1	experiments					1220:1230	the fixed-bed column experiments	1199:1230	the fixed-bed column experiments	1199:1230	The practical applicability of nZVI/BC/CA composites was further demonstrated through the fixed-bed column experiments.
30690237	0	10	dep	Cr	48:49	arg1	VI					51:52	VI	51:52	VI	51:52	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	3	11	theme	Langmuir	815:822	arg1	isotherm					824:831	the Langmuir isotherm	811:831	the Langmuir isotherm	811:831	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	1	12	theme	zero-valent	264:274	arg1	reactions					297:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	0	13	theme	iron/biochar/Ca-alginate	79:102	arg1	composite					104:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	nanoscale zerovalent iron/biochar/Ca-alginate composite	58:112	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	3	14	with	Cr	744:745	arg1	capacity					779:786	the maximum adsorption capacity	756:786	the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	756:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	4	15	theme	simultaneous	1030:1041	arg1	adsorption					1043:1052	simultaneous adsorption	1030:1052	simultaneous adsorption	1030:1052	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	1	16	theme	iron	276:279	arg1	reactions					297:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	4	17	with	reactions	1077:1085	arg1	analysis					883:890	the XPS analysis and fitting results	875:910	analysis	883:890	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	17	with	reactions	1077:1085	arg1	results					904:910	the XPS analysis and fitting results	875:910	results	904:910	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	5	18	theme	composites	1155:1164	arg1	applicability					1127:1139	The practical applicability	1113:1139	The practical applicability of nZVI/BC/CA composites	1113:1164	The practical applicability of nZVI/BC/CA composites was further demonstrated through the fixed-bed column experiments.
30690237	2	19	theme	CA	390:391	arg1	bead					394:397	calcium-alginate (CA) bead	372:397	calcium-alginate (CA) bead	372:397	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	3	20	theme	adsorption	768:777	arg1	capacity					779:786	the maximum adsorption capacity	756:786	the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	756:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	21	theme	external	690:697	arg1	CA					716:717	CA	716:717	CA	716:717	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	21	theme	external	690:697	arg1	layer					709:713	external screening layer	690:713	external screening layer (CA)	690:718	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	0	22	theme	Cr	48:49	arg1	micro-electrolysis					26:43	micro-electrolysis	26:43	micro-electrolysis	26:43	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	0	22	theme	Cr	48:49	arg1	adsorption					11:20	Concurrent adsorption	0:20	Concurrent adsorption	0:20	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	2	23	theme	nZVI-impregnated	325:340	arg1	BC					351:352	BC	351:352	BC	351:352	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	2	23	theme	nZVI-impregnated	325:340	arg1	biochar					342:348	nZVI-impregnated biochar	325:348	nZVI-impregnated biochar (BC)	325:353	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	2	24	theme	nZVI/BC/CA	440:449	arg1	composites					451:460	nZVI/BC/CA composites	440:460	nZVI/BC/CA composites	440:460	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	3	25	theme	screening	699:707	arg1	CA					716:717	CA	716:717	CA	716:717	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	25	theme	screening	699:707	arg1	layer					709:713	external screening layer	690:713	external screening layer (CA)	690:718	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	26	theme	little	838:843	arg1	release					845:851	little release	838:851	little release of dissolved Fe	838:867	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	27	theme	calcium-alginate	372:387	arg1	bead					394:397	calcium-alginate (CA) bead	372:397	calcium-alginate (CA) bead	372:397	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	0	28	theme	Concurrent	0:9	arg1	adsorption					11:20	Concurrent adsorption	0:20	Concurrent adsorption	0:20	Concurrent adsorption and micro-electrolysis of Cr(VI) by nanoscale zerovalent iron/biochar/Ca-alginate composite.
30690237	6	29	theme	high-performance	1287:1302	arg1	biochar					1315:1321	high-performance engineered biochar	1287:1321	high-performance engineered biochar for wastewater treatment	1287:1346	These results provide new insights into the design of high-performance engineered biochar for wastewater treatment.
30690237	6	30	theme	wastewater	1327:1336	arg1	treatment					1338:1346	wastewater treatment	1327:1346	wastewater treatment	1327:1346	These results provide new insights into the design of high-performance engineered biochar for wastewater treatment.
30690237	1	31	theme	nZVI	282:285	arg1	reactions					297:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	6	32	theme	engineered	1304:1313	arg1	biochar					1315:1321	high-performance engineered biochar	1287:1321	high-performance engineered biochar for wastewater treatment	1287:1346	These results provide new insights into the design of high-performance engineered biochar for wastewater treatment.
30690237	4	33	theme	Cr	1018:1019	arg1	mechanisms					1004:1013	plausible removal mechanisms	986:1013	plausible removal mechanisms of Cr	986:1019	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	1	34	theme	removal	188:194	arg1	performance					196:206	removal performance	188:206	Cr(VI) removal performance	181:206	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	2	35	theme	physicochemical	404:418	arg1	characterization					420:435	the physicochemical characterization	400:435	the physicochemical characterization of nZVI/BC/CA composites	400:460	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	4	36	theme	second	932:937	arg1	order					939:943	pseudo second order	925:943	pseudo second order	925:943	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	36	theme	second	932:937	arg1	kinetics					915:922	kinetics	915:922	kinetics (pseudo second order)	915:944	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	2	37	theme	several	547:553	arg1	cracks					555:560	several cracks	547:560	several cracks on its outer layer	547:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	3	38	theme	dissolved	856:864	arg1	Fe					866:867	dissolved Fe	856:867	dissolved Fe	856:867	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	39	theme	nZVI/BC	476:482	arg1	particles					484:492	nZVI/BC particles	476:492	nZVI/BC particles	476:492	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	1	40	theme	-mediated	287:295	arg1	reactions					297:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	nanoscale zero-valent iron (nZVI)-mediated reactions	254:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	3	41	theme	Fe	866:867	arg1	release					845:851	little release	838:851	little release of dissolved Fe	838:867	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	41	theme	Fe	866:867	arg1	removal					733:739	the removal	729:739	the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	729:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	42	theme	bead	394:397	arg1	matrix					362:367	the matrix	358:367	the matrix of calcium-alginate (CA) bead	358:397	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	3	43	theme	Cr	744:745	arg1	release					845:851	little release	838:851	little release of dissolved Fe	838:867	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	43	theme	Cr	744:745	arg1	removal					733:739	the removal	729:739	the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	729:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	44	from	cracks	555:560	arg1	layer					575:579	its outer layer	565:579	its outer layer	565:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	5	45	theme	practical	1117:1125	arg1	applicability					1127:1139	The practical applicability	1113:1139	The practical applicability of nZVI/BC/CA composites	1113:1164	The practical applicability of nZVI/BC/CA composites was further demonstrated through the fixed-bed column experiments.
30690237	6	46	theme	biochar	1315:1321	arg1	design					1277:1282	the design	1273:1282	the design of high-performance engineered biochar for wastewater treatment	1273:1346	These results provide new insights into the design of high-performance engineered biochar for wastewater treatment.
30690237	4	47	theme	XPS	879:881	arg1	analysis					883:890	the XPS analysis and fitting results	875:910	analysis	883:890	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	48	theme	fitting	896:902	arg1	results					904:910	the XPS analysis and fitting results	875:910	results	904:910	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	1	49	dep	Cr	181:182	arg1	VI					184:185	VI	184:185	VI	184:185	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	1	49	dep	Cr	181:182	arg1	performance					196:206	removal performance	188:206	Cr(VI) removal performance	181:206	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	3	50	dep	Cr	744:745	arg1	VI					747:748	VI	747:748	VI	747:748	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	4	51	with	adsorption	1043:1052	arg1	analysis					883:890	the XPS analysis and fitting results	875:910	analysis	883:890	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	51	with	adsorption	1043:1052	arg1	results					904:910	the XPS analysis and fitting results	875:910	results	904:910	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	2	52	theme	composites	451:460	arg1	characterization					420:435	the physicochemical characterization	400:435	the physicochemical characterization of nZVI/BC/CA composites	400:460	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	2	53	theme	outer	569:573	arg1	layer					575:579	its outer layer	565:579	its outer layer	565:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	4	54	theme	removal	996:1002	arg1	mechanisms					1004:1013	plausible removal mechanisms	986:1013	plausible removal mechanisms of Cr	986:1019	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	55	theme	isotherms	950:958	arg1	analysis					883:890	the XPS analysis and fitting results	875:910	analysis	883:890	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	55	theme	isotherms	950:958	arg1	results					904:910	the XPS analysis and fitting results	875:910	results	904:910	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	5	56	theme	nZVI/BC/CA	1144:1153	arg1	composites					1155:1164	nZVI/BC/CA composites	1144:1164	nZVI/BC/CA composites	1144:1164	The practical applicability of nZVI/BC/CA composites was further demonstrated through the fixed-bed column experiments.
30690237	4	57	theme	plausible	986:994	arg1	mechanisms					1004:1013	plausible removal mechanisms	986:1013	plausible removal mechanisms of Cr	986:1019	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	58	theme	pseudo	925:930	arg1	order					939:943	pseudo second order	925:943	pseudo second order	925:943	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	58	theme	pseudo	925:930	arg1	kinetics					915:922	kinetics	915:922	kinetics (pseudo second order)	915:944	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	4	59	dep	adsorption	1043:1052	arg1	VI					1021:1022	VI	1021:1022	VI	1021:1022	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	1	60	theme	new	139:141	arg1	approach					143:150	a new approach	137:150	a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions	137:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	3	61	theme	86.4 mg/g	791:799	arg1	capacity					779:786	the maximum adsorption capacity	756:786	the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	756:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	62	from	shape	537:541	arg1	layer					575:579	its outer layer	565:579	its outer layer	565:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	3	63	theme	nZVI/BC/CA	609:618	arg1	composites					620:629	nZVI/BC/CA composites	609:629	nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA)	609:718	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	2	64	contain	having	518:523	arg2	shape					537:541	a spherical shape	525:541	a spherical shape	525:541	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	2	64	contain	having	518:523	arg2	cracks					555:560	several cracks	547:560	several cracks on its outer layer	547:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	2	64	contain	having	518:523	arg1	CA					515:516	CA	515:516	CA having a spherical shape and several cracks on its outer layer	515:579	After entrapping nZVI-impregnated biochar (BC) in the matrix of calcium-alginate (CA) bead, the physicochemical characterization of nZVI/BC/CA composites revealed that nZVI/BC particles were embedded inside CA having a spherical shape and several cracks on its outer layer.
30690237	1	65	theme	dissolved	234:242	arg1	Fe					244:245	dissolved Fe	234:245	dissolved Fe	234:245	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30690237	3	66	theme	maximum	760:766	arg1	capacity					779:786	the maximum adsorption capacity	756:786	the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm)	756:832	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	3	67	theme	composites	620:629	arg1	multi-functionality					586:604	The multi-functionality	582:604	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA)	582:718	The multi-functionality of nZVI/BC/CA composites consisting of reductant (nZVI), porous adsorbent (BC), and external screening layer (CA) enhanced the removal of Cr(VI) with the maximum adsorption capacity of 86.4 mg/g (based on the Langmuir isotherm) and little release of dissolved Fe.
30690237	4	68	theme	nZVI/BC/CA	1090:1099	arg1	composites					1101:1110	nZVI/BC/CA composites	1090:1110	nZVI/BC/CA composites	1090:1110	With the XPS analysis and fitting results of kinetics (pseudo second order) and isotherms (Redlich-Peterson model), plausible removal mechanisms of Cr(VI) were simultaneous adsorption and micro-electrolysis reactions by nZVI/BC/CA composites.
30690237	1	69	theme	Fe	244:245	arg1	release					223:229	release	223:229	release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions	223:305	This study introduced a new approach for simultaneously enhancing Cr(VI) removal performance and mitigating release of dissolved Fe during nanoscale zero-valent iron (nZVI)-mediated reactions.
30672331	5	0	theme	hydrolysis	604:613	arg1	method					615:620	a new efficient two-step enzymatic hydrolysis method	569:620	a new efficient two-step enzymatic hydrolysis method	569:620	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	0	1	theme	Eleutherococcus	69:83	arg1	sessiliflorus					85:97	Eleutherococcus sessiliflorus	69:97	Eleutherococcus sessiliflorus	69:97	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	5	2	theme	same	558:561	arg1	time					563:566	the same time	554:566	the same time	554:566	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	4	3	theme	chiisanogenin	536:548	arg1	ester					527:531	β-D-glucopyranosyl ester	508:531	β-D-glucopyranosyl ester of chiisanogenin	508:548	Compound 1 was elucidated to be β-D-glucopyranosyl ester of chiisanogenin.
30672331	1	4	dep	the	284:286	arg1	leaves					288:293	leaves	288:293	leaves	288:293	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	5	theme	alcohol	265:271	arg1	extract					273:279	alcohol extract	265:279	the which alcohol extract of the leaves of Eleutherococcus sessiliflorus	255:326	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	0	6	from	the	55:57	arg1	ester					44:48	A new 3,4-seco-lupane triterpenene glycosyl ester	0:48	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.	0:98	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	5	7	theme	new	571:573	arg1	method					615:620	a new efficient two-step enzymatic hydrolysis method	569:620	a new efficient two-step enzymatic hydrolysis method	569:620	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	3	8	theme	S.Y.	345:348	arg1	Hu					350:351	S.Y. Hu	345:351	S.Y. Hu by silica gel column chromatography	345:387	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	5	9	theme	efficient	575:583	arg1	method					615:620	a new efficient two-step enzymatic hydrolysis method	569:620	a new efficient two-step enzymatic hydrolysis method	569:620	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	3	10	theme	spectroscopic	430:442	arg1	NMR					461:463	NMR	461:463	NMR	461:463	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	10	theme	spectroscopic	430:442	arg1	IR					457:458	IR	457:458	IR	457:458	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	10	theme	spectroscopic	430:442	arg1	UV					453:454	UV	453:454	UV	453:454	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	10	theme	spectroscopic	430:442	arg1	HRMS					469:472	HRMS	469:472	HRMS	469:472	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	10	theme	spectroscopic	430:442	arg1	methods					444:450	spectroscopic methods	430:450	spectroscopic methods (UV, IR, NMR and HRMS)	430:473	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	0	11	theme	new	2:4	arg1	ester					44:48	A new 3,4-seco-lupane triterpenene glycosyl ester	0:48	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.	0:98	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	5	12	theme	two-step	585:592	arg1	method					615:620	a new efficient two-step enzymatic hydrolysis method	569:620	a new efficient two-step enzymatic hydrolysis method	569:620	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	4	13	theme	β-D-glucopyranosyl	508:525	arg1	ester					527:531	β-D-glucopyranosyl ester	508:531	β-D-glucopyranosyl ester of chiisanogenin	508:548	Compound 1 was elucidated to be β-D-glucopyranosyl ester of chiisanogenin.
30672331	5	14	theme	enzymatic	594:602	arg1	method					615:620	a new efficient two-step enzymatic hydrolysis method	569:620	a new efficient two-step enzymatic hydrolysis method	569:620	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	3	15	theme	silica	356:361	arg1	chromatography					374:387	silica gel column chromatography	356:387	silica gel column chromatography	356:387	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	1	16	theme	the	284:286	arg1	extract					273:279	alcohol extract	265:279	the which alcohol extract of the leaves of Eleutherococcus sessiliflorus	255:326	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	17	theme	new	102:104	arg1	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	0	18	theme	3,4-seco-lupane	6:20	arg1	ester					44:48	A new 3,4-seco-lupane triterpenene glycosyl ester	0:48	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.	0:98	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	1	19	dep	isolated	241:248	arg1	Rupr					329:332	Rupr	329:332	Rupr	329:332	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	20	theme	minor	106:110	arg1	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	5	21	theme	→	668:668	arg1	divaroside					670:679	chiisanoside (2) → divaroside (3) → 1	651:687	chiisanoside (2) → divaroside (3) → 1	651:687	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	0	22	dep	the	55:57	arg1	leaves					59:64	leaves	59:64	leaves	59:64	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	1	23	theme	known	200:204	arg1	triterpenenes					222:234	three known 3,4-seco-lupane triterpenenes	194:234	three known 3,4-seco-lupane triterpenenes	194:234	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	0	24	theme	glycosyl	35:42	arg1	ester					44:48	A new 3,4-seco-lupane triterpenene glycosyl ester	0:48	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.	0:98	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	0	25	theme	triterpenene	22:33	arg1	ester					44:48	A new 3,4-seco-lupane triterpenene glycosyl ester	0:48	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.	0:98	A new 3,4-seco-lupane triterpenene glycosyl ester from the leaves of Eleutherococcus sessiliflorus.
30672331	1	26	theme	3,4-seco-lupane	206:220	arg1	triterpenenes					222:234	three known 3,4-seco-lupane triterpenenes	194:234	three known 3,4-seco-lupane triterpenenes	194:234	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	27	theme	3,4-seco-lupane	112:126	arg1	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	28	attach	isolated	241:248	arg2	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	28	attach	isolated	241:248	arg1	extract					273:279	alcohol extract	265:279	the which alcohol extract of the leaves of Eleutherococcus sessiliflorus	255:326	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	1	29	theme	triterpenene	128:139	arg1	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	5	30	theme	chiisanoside	651:662	arg1	divaroside					670:679	chiisanoside (2) → divaroside (3) → 1	651:687	chiisanoside (2) → divaroside (3) → 1	651:687	At the same time, a new efficient two-step enzymatic hydrolysis method was established to transform chiisanoside (2) → divaroside (3) → 1.
30672331	1	31	dep	extract	273:279	arg1	which					259:263	which	259:263	which	259:263	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	3	32	dep	methods	444:450	arg1	NMR					461:463	NMR	461:463	NMR	461:463	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	32	dep	methods	444:450	arg1	IR					457:458	IR	457:458	IR	457:458	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	32	dep	methods	444:450	arg1	UV					453:454	UV	453:454	UV	453:454	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	32	dep	methods	444:450	arg1	methods					444:450	spectroscopic methods	430:450	spectroscopic methods (UV, IR, NMR and HRMS)	430:473	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	32	dep	methods	444:450	arg1	HRMS					469:472	HRMS	469:472	HRMS	469:472	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	1	33	theme	glycosyl	141:148	arg1	ester					150:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester	100:154	A new minor 3,4-seco-lupane triterpenene glycosyl ester, named sessiloside-A1 (1), along with three known 3,4-seco-lupane triterpenenes were isolated from the which alcohol extract of the leaves of Eleutherococcus sessiliflorus (Rupr.
30672331	3	34	theme	gel	363:365	arg1	chromatography					374:387	silica gel column chromatography	356:387	silica gel column chromatography	356:387	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30672331	3	35	theme	column	367:372	arg1	chromatography					374:387	silica gel column chromatography	356:387	silica gel column chromatography	356:387	S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic methods (UV, IR, NMR and HRMS).
30498196	0	0	theme	antibody-mediated	77:93	arg1	recruitment					106:116	antibody-mediated neutrophil recruitment	77:116	antibody-mediated neutrophil recruitment	77:116	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	5	1	from	deficient	782:790	arg1	integrins					800:808	CD18 integrins	795:808	CD18 integrins	795:808	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	7	2	theme	cis	995:997	arg1	interaction					999:1009	cis interaction	995:1009	cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1	995:1063	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	4	3	theme	divalent	680:687	arg1	cations					689:695	divalent cations	680:695	divalent cations	680:695	Sialylated complex glycans on FcγRIIA interact with the αI-domain via divalent cations, and this interaction is required for FcγRIIA inhibition by Mac-1.
30498196	8	4	theme	neutrophil	1173:1182	arg1	influx					1184:1189	neutrophil influx	1173:1189	neutrophil influx	1173:1189	A disruption of this interaction may increase neutrophil influx in autoimmune diseases.
30498196	3	5	theme	Mac-1	506:510	arg1	inhibition					512:521	Mac-1 inhibition	506:521	Mac-1 inhibition of FcγRIIA	506:532	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	8	6	theme	autoimmune	1194:1203	arg1	diseases					1205:1212	autoimmune diseases	1194:1212	autoimmune diseases	1194:1212	A disruption of this interaction may increase neutrophil influx in autoimmune diseases.
30498196	1	7	theme	Vascular-deposited	119:136	arg1	complexes					149:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes promote neutrophil recruitment, but how this process is regulated is still unclear.
30498196	0	8	theme	neutrophil	95:104	arg1	recruitment					106:116	antibody-mediated neutrophil recruitment	77:116	antibody-mediated neutrophil recruitment	77:116	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	5	9	from	integrins	800:808	arg1	deficient					782:790	deficient	782:790	deficient	782:790	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	4	10	theme	FcγRIIA	735:741	arg1	inhibition					743:752	FcγRIIA inhibition	735:752	FcγRIIA inhibition by Mac-1	735:761	Sialylated complex glycans on FcγRIIA interact with the αI-domain via divalent cations, and this interaction is required for FcγRIIA inhibition by Mac-1.
30498196	3	11	dep	does	538:541	arg1	as					535:536	as	535:536	as	535:536	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	6	12	from	integrins	899:907	arg1	neutrophils					912:922	neutrophils	912:922	neutrophils	912:922	In mice, CD18 integrins on neutrophils dampen IgG-mediated neutrophil accumulation in the kidney.
30498196	2	13	theme	receptor	328:335	arg1	FcγRIIA					337:343	the IgG receptor FcγRIIA	320:343	the IgG receptor FcγRIIA in cis	320:350	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	3	14	theme	FcγRIIA	526:532	arg1	inhibition					512:521	Mac-1 inhibition	506:521	Mac-1 inhibition of FcγRIIA	506:532	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	5	15	theme	IgG-coated	861:870	arg1	endothelium					872:882	IgG-coated endothelium	861:882	IgG-coated endothelium	861:882	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	0	16	theme	Cis	0:2	arg1	interaction					4:14	Cis interaction	0:14	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1	0:68	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	3	17	theme	lupus-risk	478:487	arg1	variant					489:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	7	18	theme	neutrophil	1103:1112	arg1	recruitment					1114:1124	IgG-mediated neutrophil recruitment	1090:1124	IgG-mediated neutrophil recruitment	1090:1124	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	2	19	from	FcγRIIA	337:343	arg1	cis					348:350	cis	348:350	cis	348:350	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	6	20	theme	CD18	894:897	arg1	integrins					899:907	CD18 integrins	894:907	CD18 integrins on neutrophils	894:922	In mice, CD18 integrins on neutrophils dampen IgG-mediated neutrophil accumulation in the kidney.
30498196	2	21	theme	neutrophil	423:432	arg1	recruitment					434:444	FcγRIIA-mediated neutrophil recruitment	406:444	FcγRIIA-mediated neutrophil recruitment under flow	406:455	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	3	22	theme	Mac-1	545:549	arg1	ligand					551:556	a Mac-1 ligand	543:556	a Mac-1 ligand	543:556	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	4	23	from	glycans	629:635	arg1	FcγRIIA					640:646	FcγRIIA	640:646	FcγRIIA	640:646	Sialylated complex glycans on FcγRIIA interact with the αI-domain via divalent cations, and this interaction is required for FcγRIIA inhibition by Mac-1.
30498196	0	24	theme	sialylated	24:33	arg1	FcγRIIA					35:41	sialylated FcγRIIA	24:41	sialylated FcγRIIA	24:41	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	5	25	theme	CD18	795:798	arg1	integrins					800:808	CD18 integrins	795:808	CD18 integrins	795:808	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	3	26	from	mutation	564:571	arg1	αI-domain					599:607	Mac-1's ligand binding αI-domain	576:607	Mac-1's ligand binding αI-domain	576:607	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	4	27	theme	Sialylated	610:619	arg1	glycans					629:635	Sialylated complex glycans	610:635	Sialylated complex glycans on FcγRIIA	610:646	Sialylated complex glycans on FcγRIIA interact with the αI-domain via divalent cations, and this interaction is required for FcγRIIA inhibition by Mac-1.
30498196	7	28	theme	IgG-mediated	1090:1101	arg1	recruitment					1114:1124	IgG-mediated neutrophil recruitment	1090:1124	IgG-mediated neutrophil recruitment	1090:1124	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	2	29	theme	bent	293:296	arg1	state					298:302	its bent state	289:302	its bent state	289:302	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	5	30	theme	FcγRIIA-dependent	828:844	arg1	recruitment					846:856	augmented FcγRIIA-dependent recruitment	818:856	augmented FcγRIIA-dependent recruitment to IgG-coated endothelium	818:882	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	2	31	theme	IgG	324:326	arg1	FcγRIIA					337:343	the IgG receptor FcγRIIA	320:343	the IgG receptor FcγRIIA in cis	320:350	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	5	32	theme	Human	764:768	arg1	neutrophils					770:780	Human neutrophils	764:780	Human neutrophils deficient in CD18 integrins	764:808	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	2	33	theme	FcγRIIA-mediated	406:421	arg1	recruitment					434:444	FcγRIIA-mediated neutrophil recruitment	406:444	FcγRIIA-mediated neutrophil recruitment under flow	406:455	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	2	34	theme	FcγRIIA	378:384	arg1	affinity					366:373	the affinity	362:373	the affinity of FcγRIIA for IgG	362:392	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	7	35	theme	Mac-1	1059:1063	arg1	Mac-1					1059:1063	Mac-1	1059:1063	Mac-1	1059:1063	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	7	35	theme	Mac-1	1059:1063	arg1	FcγRIIA					1030:1036	sialylated FcγRIIA	1019:1036	sialylated FcγRIIA	1019:1036	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	7	35	theme	Mac-1	1059:1063	arg1	αI-domain					1046:1054	the αI-domain	1042:1054	the αI-domain of Mac-1	1042:1063	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	1	36	theme	IgG	138:140	arg1	complexes					149:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes promote neutrophil recruitment, but how this process is regulated is still unclear.
30498196	5	37	theme	deficient	782:790	arg1	neutrophils					770:780	Human neutrophils	764:780	Human neutrophils deficient in CD18 integrins	764:808	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	0	38	gly	sialylated	24:33	arg1	FcγRIIA					35:41	sialylated FcγRIIA	24:41	sialylated FcγRIIA	24:41	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	7	39	theme	sialylated	1019:1028	arg1	FcγRIIA					1030:1036	sialylated FcγRIIA	1019:1036	sialylated FcγRIIA	1019:1036	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	1	40	theme	immune	142:147	arg1	complexes					149:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes	119:157	Vascular-deposited IgG immune complexes promote neutrophil recruitment, but how this process is regulated is still unclear.
30498196	6	41	theme	neutrophil	944:953	arg1	accumulation					955:966	IgG-mediated neutrophil accumulation	931:966	IgG-mediated neutrophil accumulation	931:966	In mice, CD18 integrins on neutrophils dampen IgG-mediated neutrophil accumulation in the kidney.
30498196	8	42	theme	interaction	1148:1158	arg1	disruption					1129:1138	A disruption	1127:1138	A disruption of this interaction	1127:1158	A disruption of this interaction may increase neutrophil influx in autoimmune diseases.
30498196	3	43	theme	binding	591:597	arg1	αI-domain					599:607	Mac-1's ligand binding αI-domain	576:607	Mac-1's ligand binding αI-domain	576:607	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	2	44	theme	integrin	270:277	arg1	Mac-1					279:283	the CD18 integrin Mac-1	261:283	the CD18 integrin Mac-1	261:283	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	7	45	gly	sialylated	1019:1028	arg1	FcγRIIA					1030:1036	sialylated FcγRIIA	1019:1036	sialylated FcγRIIA	1019:1036	In summary, cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 alters the threshold for IgG-mediated neutrophil recruitment.
30498196	3	46	theme	Mac-1	462:466	arg1	variant					489:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	2	47	from	Mac-1	279:283	arg1	state					298:302	its bent state	289:302	its bent state	289:302	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	2	48	theme	CD18	265:268	arg1	Mac-1					279:283	the CD18 integrin Mac-1	261:283	the CD18 integrin Mac-1	261:283	Here we show that the CD18 integrin Mac-1, in its bent state, interacts with the IgG receptor FcγRIIA in cis to reduce the affinity of FcγRIIA for IgG and inhibit FcγRIIA-mediated neutrophil recruitment under flow.
30498196	3	49	theme	rs1143679	468:476	arg1	variant					489:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant	458:495	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	3	50	from	ligand	551:556	arg1	αI-domain					599:607	Mac-1's ligand binding αI-domain	576:607	Mac-1's ligand binding αI-domain	576:607	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
30498196	0	51	theme	Mac-1	64:68	arg1	Mac-1					64:68	Mac-1	64:68	Mac-1	64:68	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	0	51	theme	Mac-1	64:68	arg1	FcγRIIA					35:41	sialylated FcγRIIA	24:41	sialylated FcγRIIA	24:41	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	0	51	theme	Mac-1	64:68	arg1	αI-domain					51:59	the αI-domain	47:59	the αI-domain of Mac-1	47:68	Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
30498196	4	52	theme	complex	621:627	arg1	glycans					629:635	Sialylated complex glycans	610:635	Sialylated complex glycans on FcγRIIA	610:646	Sialylated complex glycans on FcγRIIA interact with the αI-domain via divalent cations, and this interaction is required for FcγRIIA inhibition by Mac-1.
30498196	5	53	theme	augmented	818:826	arg1	recruitment					846:856	augmented FcγRIIA-dependent recruitment	818:856	augmented FcγRIIA-dependent recruitment to IgG-coated endothelium	818:882	Human neutrophils deficient in CD18 integrins exhibit augmented FcγRIIA-dependent recruitment to IgG-coated endothelium.
30498196	1	54	theme	neutrophil	167:176	arg1	recruitment					178:188	neutrophil recruitment	167:188	neutrophil recruitment	167:188	Vascular-deposited IgG immune complexes promote neutrophil recruitment, but how this process is regulated is still unclear.
30498196	6	55	theme	IgG-mediated	931:942	arg1	accumulation					955:966	IgG-mediated neutrophil accumulation	931:966	IgG-mediated neutrophil accumulation	931:966	In mice, CD18 integrins on neutrophils dampen IgG-mediated neutrophil accumulation in the kidney.
30498196	3	56	theme	ligand	584:589	arg1	αI-domain					599:607	Mac-1's ligand binding αI-domain	576:607	Mac-1's ligand binding αI-domain	576:607	The Mac-1 rs1143679 lupus-risk variant reverses Mac-1 inhibition of FcγRIIA, as does a Mac-1 ligand and a mutation in Mac-1's ligand binding αI-domain.
31004639	8	0	theme	EPSs	1058:1061	arg1	activity					1042:1049	the anti-inflammatory activity	1020:1049	the anti-inflammatory activity of the EPSs	1020:1061	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	3	1	theme	factor	513:518	arg1	secretion					530:538	the lowest tumour necrosis factor α (TNF-α) secretion	486:538	the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA)	486:565	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	1	theme	factor	513:518	arg1	DNA					562:564	325.32 ± 51.10 pg/ug DNA	541:564	325.32 ± 51.10 pg/ug DNA	541:564	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	4	2	theme	lowest	579:584	arg1	secretion					593:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	6	3	theme	TNF-α	764:768	arg1	secretion					770:778	the lowest TNF-α secretion	753:778	the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA)	753:812	Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA).
31004639	9	4	theme	anti-inflammatory	1109:1125	arg1	effects					1127:1133	their anti-inflammatory effects	1103:1133	their anti-inflammatory effects on the macrophages	1103:1152	The galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages.
31004639	7	5	dep	had	863:865	arg1	%					907:907	45 ± 2.75%	898:907	45 ± 2.75%	898:907	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	5	dep	had	863:865	arg1	%					922:922	39 ± 2.75%	913:922	39 ± 2.75%	913:922	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	4	6	theme	second	572:577	arg1	secretion					593:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	2	7	theme	exopolysaccharide	247:263	arg1	production					271:280	high exopolysaccharide (EPS) production	242:280	high exopolysaccharide (EPS) production ability	242:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	7	8	theme	monosaccharide	819:832	arg1	components					834:843	The monosaccharide components	815:843	The monosaccharide components	815:843	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	8	theme	monosaccharide	819:832	arg1	EPS					846:848	EPS 4	846:850	EPS 4	846:850	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	8	theme	monosaccharide	819:832	arg1	EPS					856:858	EPS 1	856:860	EPS 1	856:860	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	4	9	theme	α	591:591	arg1	secretion					593:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	8	10	theme	>	982:982	arg1	percentages					959:969	The monosaccharide percentages	940:969	The monosaccharide percentages (galactose > rhamnose > glucose)	940:1002	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	10	theme	>	982:982	arg1	glucose					995:1001	galactose > rhamnose > glucose	972:1001	galactose > rhamnose > glucose	972:1001	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	11	theme	monosaccharide	944:957	arg1	percentages					959:969	The monosaccharide percentages	940:969	The monosaccharide percentages (galactose > rhamnose > glucose)	940:1002	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	11	theme	monosaccharide	944:957	arg1	glucose					995:1001	galactose > rhamnose > glucose	972:1001	galactose > rhamnose > glucose	972:1001	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	11	theme	monosaccharide	944:957	arg1	related					1009:1015	related	1009:1015	related	1009:1015	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	7	12	theme	highest	871:877	arg1	content					889:895	the highest galactose content	867:895	the highest galactose content	867:895	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	3	13	theme	325.32 ± 51.10 pg/ug	541:560	arg1	secretion					530:538	the lowest tumour necrosis factor α (TNF-α) secretion	486:538	the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA)	486:565	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	13	theme	325.32 ± 51.10 pg/ug	541:560	arg1	DNA					562:564	325.32 ± 51.10 pg/ug DNA	541:564	325.32 ± 51.10 pg/ug DNA	541:564	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	14	theme	lowest	490:495	arg1	secretion					530:538	the lowest tumour necrosis factor α (TNF-α) secretion	486:538	the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA)	486:565	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	14	theme	lowest	490:495	arg1	DNA					562:564	325.32 ± 51.10 pg/ug DNA	541:564	325.32 ± 51.10 pg/ug DNA	541:564	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	9	15	from	effects	1127:1133	arg1	macrophages					1142:1152	the macrophages	1138:1152	the macrophages	1138:1152	The galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages.
31004639	8	16	theme	galactose	972:980	arg1	percentages					959:969	The monosaccharide percentages	940:969	The monosaccharide percentages (galactose > rhamnose > glucose)	940:1002	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	16	theme	galactose	972:980	arg1	glucose					995:1001	galactose > rhamnose > glucose	972:1001	galactose > rhamnose > glucose	972:1001	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	7	17	theme	galactose	879:887	arg1	content					889:895	the highest galactose content	867:895	the highest galactose content	867:895	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	4	18	from	Mh-002	656:661	arg1	DNA					646:648	701.12 ± 86.108 pg/ug DNA	624:648	701.12 ± 86.108 pg/ug DNA	624:648	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	4	18	from	Mh-002	656:661	arg1	EPS					617:619	EPS 2	617:621	EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002	617:661	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	4	19	theme	TNF-	586:589	arg1	secretion					593:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion	568:601	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	6	20	theme	EPS	743:745	arg1	1 ppm					734:738	1 ppm	734:738	1 ppm of EPS	734:745	Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA).
31004639	3	21	theme	unpurified	404:413	arg1	EPSs					415:418	the three unpurified EPSs	394:418	the three unpurified EPSs	394:418	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	10	22	theme	physiological	1205:1217	arg1	effects					1219:1225	beneficial physiological effects	1194:1225	beneficial physiological effects such as anti-inflammatory properties	1194:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	22	theme	physiological	1205:1217	arg1	properties					1253:1262	anti-inflammatory properties	1235:1262	anti-inflammatory properties	1235:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	8	23	theme	rhamnose	984:991	arg1	percentages					959:969	The monosaccharide percentages	940:969	The monosaccharide percentages (galactose > rhamnose > glucose)	940:1002	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	23	theme	rhamnose	984:991	arg1	glucose					995:1001	galactose > rhamnose > glucose	972:1001	galactose > rhamnose > glucose	972:1001	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	10	24	theme	beneficial	1194:1203	arg1	effects					1219:1225	beneficial physiological effects	1194:1225	beneficial physiological effects such as anti-inflammatory properties	1194:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	24	theme	beneficial	1194:1203	arg1	properties					1253:1262	anti-inflammatory properties	1235:1262	anti-inflammatory properties	1235:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	0	25	theme	Monosaccharide	0:13	arg1	composition					15:25	Monosaccharide composition	0:25	Monosaccharide composition	0:25	Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides.
31004639	2	26	theme	Lactobacillus	208:220	arg1	Mh-001					230:235	Lactobacillus reuteri Mh-001	208:235	Lactobacillus reuteri Mh-001	208:235	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	10	27	theme	EPS	1303:1305	arg1	content					1288:1294	the monosaccharide content	1269:1294	the monosaccharide content of the EPS	1269:1305	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	27	theme	EPS	1303:1305	arg1	factor					1315:1320	the factor	1311:1320	the factor influencing the anti-inflammatory properties	1311:1365	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	28	theme	anti-inflammatory	1338:1354	arg1	properties					1356:1365	the anti-inflammatory properties	1334:1365	the anti-inflammatory properties	1334:1365	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	9	29	theme	EPSs	1089:1092	arg1	content					1078:1084	The galactose content	1064:1084	The galactose content of EPSs	1064:1092	The galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages.
31004639	7	30	contain	had	863:865	arg1	components					834:843	The monosaccharide components	815:843	The monosaccharide components	815:843	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	30	contain	had	863:865	arg2	content					889:895	the highest galactose content	867:895	the highest galactose content	867:895	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	30	contain	had	863:865	arg1	EPS					846:848	EPS 4	846:850	EPS 4	846:850	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	7	30	contain	had	863:865	arg1	EPS					856:858	EPS 1	856:860	EPS 1	856:860	The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively).
31004639	8	31	theme	>	993:993	arg1	percentages					959:969	The monosaccharide percentages	940:969	The monosaccharide percentages (galactose > rhamnose > glucose)	940:1002	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	8	31	theme	>	993:993	arg1	glucose					995:1001	galactose > rhamnose > glucose	972:1001	galactose > rhamnose > glucose	972:1001	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	10	32	theme	anti-inflammatory	1235:1251	arg1	properties					1253:1262	anti-inflammatory properties	1235:1262	anti-inflammatory properties	1235:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	3	33	theme	necrosis	504:511	arg1	factor					513:518	tumour necrosis factor α	497:520	the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA)	486:565	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	33	theme	necrosis	504:511	arg1	TNF-α					523:527	TNF-α	523:527	TNF-α	523:527	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	4	34	theme	701.12 ± 86.108 pg/ug	624:644	arg1	DNA					646:648	701.12 ± 86.108 pg/ug DNA	624:648	701.12 ± 86.108 pg/ug DNA	624:648	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	4	34	theme	701.12 ± 86.108 pg/ug	624:644	arg1	EPS					617:619	EPS 2	617:621	EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002	617:661	The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002.
31004639	3	35	theme	murine	430:435	arg1	macrophages					437:447	RAW246.7 murine macrophages	421:447	RAW246.7 murine macrophages treated with 5 ppm of EPS 1	421:475	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	6	36	theme	DNA	809:811	arg1	secretion					770:778	the lowest TNF-α secretion	753:778	the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA)	753:812	Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA).
31004639	3	37	theme	EPS	471:473	arg1	5 ppm					462:466	5 ppm	462:466	5 ppm of EPS 1	462:475	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	0	38	theme	probiotic	69:77	arg1	exopolysaccharides					79:96	probiotic exopolysaccharides	69:96	probiotic exopolysaccharides	69:96	Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides.
31004639	6	39	theme	209.20 ± 84.34 pg/ug	788:807	arg1	DNA					809:811	all (209.20 ± 84.34 pg/ug DNA)	783:812	all (209.20 ± 84.34 pg/ug DNA)	783:812	Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA).
31004639	0	40	dep	effects	58:64	arg1	immunomodulatory					41:56	immunomodulatory	41:56	immunomodulatory	41:56	Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides.
31004639	10	41	theme	monosaccharide	1273:1286	arg1	content					1288:1294	the monosaccharide content	1269:1294	the monosaccharide content of the EPS	1269:1305	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	41	theme	monosaccharide	1273:1286	arg1	factor					1315:1320	the factor	1311:1320	the factor influencing the anti-inflammatory properties	1311:1365	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	3	42	theme	tumour	497:502	arg1	factor					513:518	tumour necrosis factor α	497:520	the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA)	486:565	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	3	42	theme	tumour	497:502	arg1	TNF-α					523:527	TNF-α	523:527	TNF-α	523:527	Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA).
31004639	10	43	contain	possesses	1184:1192	arg2	properties					1253:1262	anti-inflammatory properties	1235:1262	anti-inflammatory properties	1235:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	43	contain	possesses	1184:1192	arg1	EPS					1180:1182	EPS	1180:1182	EPS	1180:1182	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	10	43	contain	possesses	1184:1192	arg2	effects					1219:1225	beneficial physiological effects	1194:1225	beneficial physiological effects such as anti-inflammatory properties	1194:1262	These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
31004639	2	44	theme	high	242:245	arg1	production					271:280	high exopolysaccharide (EPS) production	242:280	high exopolysaccharide (EPS) production ability	242:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	1	45	theme	probiotics	144:153	arg1	Exopolysaccharides					99:116	Exopolysaccharides	99:116	Exopolysaccharides (EPSs)	99:123	Exopolysaccharides (EPSs) are metabolites of probiotics that have gained wide interest recently.
31004639	1	45	theme	probiotics	144:153	arg1	metabolites					129:139	metabolites	129:139	metabolites of probiotics that have gained wide interest recently	129:193	Exopolysaccharides (EPSs) are metabolites of probiotics that have gained wide interest recently.
31004639	8	46	theme	anti-inflammatory	1024:1040	arg1	activity					1042:1049	the anti-inflammatory activity	1020:1049	the anti-inflammatory activity of the EPSs	1020:1061	The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs.
31004639	2	47	theme	Mh-001	230:235	arg1	strain					198:203	A strain	196:203	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability	196:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	6	48	theme	lowest	757:762	arg1	secretion					770:778	the lowest TNF-α secretion	753:778	the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA)	753:812	Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA).
31004639	2	49	with	strain	198:203	arg1	ability					282:288	high exopolysaccharide (EPS) production ability	242:288	high exopolysaccharide (EPS) production ability	242:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	2	50	used	used	325:328	arg2	strain					198:203	A strain	196:203	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability	196:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	2	51	dep	Lactobacillus	208:220	arg1	reuteri					222:228	reuteri	222:228	reuteri	222:228	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	2	52	theme	anti-inflammatory	349:365	arg1	effects					367:373	the anti-inflammatory effects	345:373	the anti-inflammatory effects of the EPSs	345:385	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	2	53	theme	production	271:280	arg1	ability					282:288	high exopolysaccharide (EPS) production ability	242:288	high exopolysaccharide (EPS) production ability	242:288	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
31004639	1	54	theme	wide	172:175	arg1	interest					177:184	wide interest	172:184	wide interest	172:184	Exopolysaccharides (EPSs) are metabolites of probiotics that have gained wide interest recently.
31004639	0	55	theme	exopolysaccharides	79:96	arg1	effects					58:64	and immunomodulatory effects	37:64	effects	58:64	Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides.
31004639	9	56	theme	galactose	1068:1076	arg1	content					1078:1084	The galactose content	1064:1084	The galactose content of EPSs	1064:1092	The galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages.
31004639	2	57	theme	EPSs	382:385	arg1	effects					367:373	the anti-inflammatory effects	345:373	the anti-inflammatory effects of the EPSs	345:385	A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs.
30529353	2	0	theme	resonance	517:525	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	0	theme	resonance	517:525	arg1	spectroscopy					533:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	1	1	theme	seed	377:380	arg1	cake					382:385	Camellia oleifera Abel seed cake	354:385	Camellia oleifera Abel seed cake	354:385	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	4	2	theme	SCP-2	1095:1099	arg1	doses					1086:1090	high, medium and low doses	1065:1090	high, medium and low doses of SCP-2	1065:1099	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	1	3	theme	polysaccharide	181:194	arg1	fractions					196:204	Two homogeneous polysaccharide fractions	165:204	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da)	165:258	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	2	4	theme	magnetic	508:515	arg1	NMR					528:530	NMR	528:530	NMR	528:530	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	4	theme	magnetic	508:515	arg1	resonance					517:525	nuclear magnetic resonance	500:525	nuclear magnetic resonance (NMR) spectroscopy	500:544	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	4	5	theme	hyperglycemia	881:893	arg1	mice					895:898	hyperglycemia mice	881:898	hyperglycemia mice	881:898	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	5	6	theme	hyperglycemia	1310:1322	arg1	mice					1324:1327	the hyperglycemia mice	1306:1327	the hyperglycemia mice	1306:1327	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	7	7	theme	hypoglycemic	1471:1482	arg1	stronger					1506:1513	stronger	1506:1513	stronger	1506:1513	In addition, the hypoglycemic activity of SCP-1 was stronger than that of SCP-2.
30529353	7	7	theme	hypoglycemic	1471:1482	arg1	activity					1484:1491	the hypoglycemic activity	1467:1491	the hypoglycemic activity of SCP-1	1467:1500	In addition, the hypoglycemic activity of SCP-1 was stronger than that of SCP-2.
30529353	0	8	theme	hypoglycemic	102:113	arg1	activity					115:122	its hypoglycemic activity	98:122	its hypoglycemic activity	98:122	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
30529353	6	9	theme	strong	1385:1390	arg1	activities					1405:1414	strong hypoglycemic activities	1385:1414	strong hypoglycemic activities in streptozotocin-induced model mice	1385:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	3	10	with	d‑glucose	648:656	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	10	with	d‑glucose	648:656	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	5	11	theme	dismutase	1228:1236	arg1	activities					1151:1160	the activities	1147:1160	the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)	1147:1242	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	4	12	theme	medium	1071:1076	arg1	doses					1086:1090	high, medium and low doses	1065:1090	high, medium and low doses of SCP-2	1065:1099	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	13	from	levels	871:876	arg1	mice					895:898	hyperglycemia mice	881:898	hyperglycemia mice	881:898	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	14	theme	glucose	863:869	arg1	levels					871:876	the plasma glucose levels	852:876	the plasma glucose levels in hyperglycemia mice	852:898	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	15	theme	plasma	856:861	arg1	levels					871:876	the plasma glucose levels	852:876	the plasma glucose levels in hyperglycemia mice	852:898	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	16	theme	low	1082:1084	arg1	doses					1086:1090	high, medium and low doses	1065:1090	high, medium and low doses of SCP-2	1065:1099	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	17	theme	medium	969:974	arg1	doses					984:988	high, medium and low doses	963:988	high, medium and low doses of SCP-1	963:997	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	3	18	with	d‑glucose	755:763	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	18	with	d‑glucose	755:763	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	6	19	from	activities	1405:1414	arg1	mice					1448:1451	streptozotocin-induced model mice	1419:1451	streptozotocin-induced model mice	1419:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	6	20	theme	model	1442:1446	arg1	mice					1448:1451	streptozotocin-induced model mice	1419:1451	streptozotocin-induced model mice	1419:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	2	21	theme	transformed	423:433	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	21	theme	transformed	423:433	arg1	spectroscopy					533:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	21	theme	transformed	423:433	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC)	453:497	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	21	theme	transformed	423:433	arg1	calorimetry					572:582	differential scanning calorimetry	550:582	differential scanning calorimetry (DSC)	550:588	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	21	theme	transformed	423:433	arg1	FT-IR					445:449	FT-IR	445:449	FT-IR	445:449	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	0	22	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel	0:82	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
30529353	1	23	theme	Sephadex	299:306	arg1	chromatography					321:334	DEAE-52 cellulose and Sephadex G-200 column chromatography	277:334	chromatography	321:334	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	3	24	theme	monosaccharide	595:608	arg1	compositions					610:621	The monosaccharide compositions	591:621	The monosaccharide compositions of SCP-1	591:630	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	24	theme	monosaccharide	595:608	arg1	d‑mannose					637:645	d‑mannose	637:645	d‑mannose	637:645	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	25	with	l‑xylose	662:669	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	25	with	l‑xylose	662:669	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	2	26	theme	Fourier	415:421	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	26	theme	Fourier	415:421	arg1	spectroscopy					533:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	nuclear magnetic resonance (NMR) spectroscopy	500:544	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	26	theme	Fourier	415:421	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC)	453:497	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	26	theme	Fourier	415:421	arg1	calorimetry					572:582	differential scanning calorimetry	550:582	differential scanning calorimetry (DSC)	550:588	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	26	theme	Fourier	415:421	arg1	FT-IR					445:449	FT-IR	445:449	FT-IR	445:449	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	27	theme	liquid	470:475	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC)	453:497	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	27	theme	liquid	470:475	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	27	theme	liquid	470:475	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	1	28	theme	G-200	308:312	arg1	chromatography					321:334	DEAE-52 cellulose and Sephadex G-200 column chromatography	277:334	chromatography	321:334	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	0	29	theme	diabetic	150:157	arg1	mice					159:162	streptozotocin-induced diabetic mice	127:162	streptozotocin-induced diabetic mice	127:162	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
30529353	3	30	with	l‑xylose	769:776	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	30	with	l‑xylose	769:776	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	5	31	theme	superoxide	1217:1226	arg1	dismutase					1228:1236	superoxide dismutase	1217:1236	superoxide dismutase (SOD)	1217:1242	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	5	31	theme	superoxide	1217:1226	arg1	SOD					1239:1241	SOD	1239:1241	SOD	1239:1241	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	2	32	theme	high	453:456	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC)	453:497	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	32	theme	high	453:456	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	32	theme	high	453:456	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	33	theme	performance	458:468	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC)	453:497	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	33	theme	performance	458:468	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	33	theme	performance	458:468	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	1	34	theme	column	314:319	arg1	chromatography					321:334	DEAE-52 cellulose and Sephadex G-200 column chromatography	277:334	chromatography	321:334	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	0	35	theme	streptozotocin-induced	127:148	arg1	mice					159:162	streptozotocin-induced diabetic mice	127:162	streptozotocin-induced diabetic mice	127:162	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
30529353	4	36	theme	high	1065:1068	arg1	doses					1086:1090	high, medium and low doses	1065:1090	high, medium and low doses of SCP-2	1065:1099	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	6	37	theme	streptozotocin-induced	1419:1440	arg1	mice					1448:1451	streptozotocin-induced model mice	1419:1451	streptozotocin-induced model mice	1419:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	2	38	theme	nuclear	500:506	arg1	NMR					528:530	NMR	528:530	NMR	528:530	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	38	theme	nuclear	500:506	arg1	resonance					517:525	nuclear magnetic resonance	500:525	nuclear magnetic resonance (NMR) spectroscopy	500:544	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	6	39	contain	possessed	1375:1383	arg1	SCP-2					1369:1373	SCP-2	1369:1373	SCP-2	1369:1373	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	6	39	contain	possessed	1375:1383	arg1	SCP-1					1359:1363	SCP-1	1359:1363	SCP-1	1359:1363	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	6	39	contain	possessed	1375:1383	arg2	activities					1405:1414	strong hypoglycemic activities	1385:1414	strong hypoglycemic activities in streptozotocin-induced model mice	1385:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	3	40	theme	molar	678:682	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	41	theme	SCP-1	626:630	arg1	compositions					610:621	The monosaccharide compositions	591:621	The monosaccharide compositions of SCP-1	591:630	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	41	theme	SCP-1	626:630	arg1	d‑mannose					637:645	d‑mannose	637:645	d‑mannose	637:645	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	6	42	theme	hypoglycemic	1392:1403	arg1	activities					1405:1414	strong hypoglycemic activities	1385:1414	strong hypoglycemic activities in streptozotocin-induced model mice	1385:1451	These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice.
30529353	5	43	from	content	1270:1276	arg1	mice					1324:1327	the hyperglycemia mice	1306:1327	the hyperglycemia mice	1306:1327	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	4	44	theme	low	980:982	arg1	doses					984:988	high, medium and low doses	963:988	high, medium and low doses of SCP-1	963:997	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	3	45	theme	molar	785:789	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	2	46	theme	scanning	563:570	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	46	theme	scanning	563:570	arg1	DSC					585:587	DSC	585:587	DSC	585:587	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	46	theme	scanning	563:570	arg1	calorimetry					572:582	differential scanning calorimetry	550:582	differential scanning calorimetry (DSC)	550:588	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	0	47	theme	polysaccharides	30:44	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel	0:82	Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
30529353	4	48	theme	SCP-1	993:997	arg1	doses					984:988	high, medium and low doses	963:988	high, medium and low doses of SCP-1	963:997	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	2	49	theme	differential	550:561	arg1	infrared					435:442	Fourier transformed infrared	415:442	Fourier transformed infrared (FT-IR)	415:450	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	49	theme	differential	550:561	arg1	DSC					585:587	DSC	585:587	DSC	585:587	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	2	49	theme	differential	550:561	arg1	calorimetry					572:582	differential scanning calorimetry	550:582	differential scanning calorimetry (DSC)	550:588	They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC).
30529353	3	50	theme	1.77:0.93:1	693:703	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	5	51	theme	malondialdehyde	1281:1295	arg1	content					1270:1276	the content	1266:1276	the content of malondialdehyde (MDA) in the hyperglycemia mice	1266:1327	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	5	52	theme	catalase	1198:1205	arg1	activities					1151:1160	the activities	1147:1160	the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)	1147:1242	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	3	53	with	d‑mannose	732:740	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	53	with	d‑mannose	732:740	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	7	54	theme	SCP-1	1496:1500	arg1	stronger					1506:1513	stronger	1506:1513	stronger	1506:1513	In addition, the hypoglycemic activity of SCP-1 was stronger than that of SCP-2.
30529353	7	54	theme	SCP-1	1496:1500	arg1	activity					1484:1491	the hypoglycemic activity	1467:1491	the hypoglycemic activity of SCP-1	1467:1500	In addition, the hypoglycemic activity of SCP-1 was stronger than that of SCP-2.
30529353	4	55	theme	doses	984:988	arg1	administration					945:958	administration	945:958	administration of high, medium and low doses of SCP-1	945:997	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	3	56	with	d‑mannose	637:645	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	56	with	d‑mannose	637:645	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	4	57	theme	Animal	818:823	arg1	experiments					825:835	Animal experiments	818:835	Animal experiments	818:835	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	4	58	theme	doses	1086:1090	arg1	administration					1047:1060	administration	1047:1060	administration of high, medium and low doses of SCP-2	1047:1099	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	3	59	with	l‑rhamnose	743:752	arg1	ratio					684:688	a molar ratio	676:688	a molar ratio of 1.77:0.93:1	676:703	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	3	59	with	l‑rhamnose	743:752	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
30529353	5	60	theme	glutathione	1165:1175	arg1	GSH-Px					1189:1194	GSH-Px	1189:1194	GSH-Px	1189:1194	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	5	60	theme	glutathione	1165:1175	arg1	peroxidase					1177:1186	glutathione peroxidase	1165:1186	glutathione peroxidase (GSH-Px)	1165:1195	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	4	61	theme	high	963:966	arg1	doses					984:988	high, medium and low doses	963:988	high, medium and low doses of SCP-1	963:997	Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively.
30529353	1	62	theme	Abel	372:375	arg1	cake					382:385	Camellia oleifera Abel seed cake	354:385	Camellia oleifera Abel seed cake	354:385	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	5	63	theme	peroxidase	1177:1186	arg1	activities					1151:1160	the activities	1147:1160	the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)	1147:1242	SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice.
30529353	1	64	theme	homogeneous	169:179	arg1	fractions					196:204	Two homogeneous polysaccharide fractions	165:204	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da)	165:258	Two homogeneous polysaccharide fractions named SCP-1 (7.16 × 106 Da) and SCP-2 (2.00 × 104 Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake.
30529353	3	65	theme	5.27:1.21:0.16:1	800:815	arg1	ratio					791:795	a molar ratio	783:795	a molar ratio of 5.27:1.21:0.16:1	783:815	The monosaccharide compositions of SCP-1 were d‑mannose, d‑glucose and l‑xylose with a molar ratio of 1.77:0.93:1 and that of the SCP-2 were d‑mannose, l‑rhamnose, d‑glucose and l‑xylose with a molar ratio of 5.27:1.21:0.16:1.
32237352	13	0	theme	different	2880:2888	arg1	products					2900:2907	different processed products	2880:2907	different processed products of Polygoni Multiflori Radix	2880:2936	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	9	1	theme	relative	1630:1637	arg1	areas					1644:1648	the relative peak areas	1626:1648	the relative peak areas of 12 peaks in the processed products	1626:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	1	theme	relative	1630:1637	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	6	2	theme	six-sun-cured	1149:1161	arg1	products					1163:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	6	2	theme	six-sun-cured	1149:1161	arg1	point					1201:1205	the finishing point	1187:1205	the finishing point for the classic steaming	1187:1230	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	12	3	theme	verification	2443:2454	arg1	requirements					2456:2467	the methodological verification requirements	2424:2467	the methodological verification requirements	2424:2467	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	3	4	theme	chemical	541:548	arg1	fingerprints					550:561	HPLC chemical fingerprints	536:561	HPLC chemical fingerprints	536:561	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	1	5	theme	chemical	247:254	arg1	changes					268:274	the chemical composition changes	243:274	the chemical composition changes	243:274	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	12	6	theme	content	2509:2515	arg1	determination					2517:2529	multi-component content determination	2493:2529	multi-component content determination combined with fingerprint	2493:2555	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	7	theme	composition	2726:2736	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	7	theme	composition	2726:2736	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	7	theme	composition	2726:2736	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	7	theme	composition	2726:2736	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	11	8	theme	stilbene	2259:2266	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	8	theme	stilbene	2259:2266	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	8	theme	stilbene	2259:2266	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	8	theme	stilbene	2259:2266	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	3	9	theme	orthogonal	584:593	arg1	OPLS-DA					640:646	OPLS-DA	640:646	OPLS-DA	640:646	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	3	9	theme	orthogonal	584:593	arg1	analysis					630:637	orthogonal partial least squares-discriminant analysis	584:637	orthogonal partial least squares-discriminant analysis( OPLS-DA)	584:647	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	13	10	theme	quality	2849:2855	arg1	model					2868:2872	the chemical quality evaluation model	2836:2872	the chemical quality evaluation model	2836:2872	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	9	11	theme	sample	1890:1895	arg1	S1					1897:1898	sample S1	1890:1898	sample S1	1890:1898	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	12	12	theme	dynamic	2699:2705	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	12	theme	dynamic	2699:2705	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	12	theme	dynamic	2699:2705	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	12	theme	dynamic	2699:2705	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	9	13	theme	processed	1669:1677	arg1	products					1679:1686	the processed products	1665:1686	the processed products	1665:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	0	14	theme	classic	114:120	arg1	method					133:138	its classic processing method	110:138	its classic processing method	110:138	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	3	15	theme	least	603:607	arg1	OPLS-DA					640:646	OPLS-DA	640:646	OPLS-DA	640:646	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	3	15	theme	least	603:607	arg1	analysis					630:637	orthogonal partial least squares-discriminant analysis	584:637	orthogonal partial least squares-discriminant analysis( OPLS-DA)	584:647	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	5	16	theme	times	880:884	arg1	number					846:851	the number	842:851	the number of processing and steaming times	842:884	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	17	from	peaks	1656:1660	arg1	products					1679:1686	the processed products	1665:1686	the processed products	1665:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	18	theme	processing	856:865	arg1	number					846:851	the number	842:851	the number of processing and steaming times	842:884	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	19	theme	peak	1505:1508	arg1	areas					1510:1514	the relative peak areas	1492:1514	the relative peak areas of the 26 chromatographic peaks in the sample	1492:1560	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	12	20	theme	classical	2632:2640	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	20	theme	classical	2632:2640	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	20	theme	classical	2632:2640	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	20	theme	classical	2632:2640	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	9	21	dep	normalization	1568:1580	arg1	reference					1587:1595	the reference	1583:1595	the reference is peak 7	1583:1605	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	0	22	theme	nine-steaming	141:153	arg1	"					174:174	nine-steaming and nine-sun-curing"	141:174	nine-steaming and nine-sun-curing"	141:174	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	9	23	theme	raw	1709:1711	arg1	peaks					1725:1729	the raw products,13 peaks	1705:1729	the raw products,13 peaks	1705:1729	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	0	24	theme	nine-sun-curing	159:173	arg1	"					174:174	nine-steaming and nine-sun-curing"	141:174	nine-steaming and nine-sun-curing"	141:174	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	3	25	theme	processed	676:684	arg1	products					686:693	different processed products	666:693	different processed products using SIMCA 14	666:708	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	0	26	from	degrees	93:99	arg1	profile					32:38	chemical quality profile	15:38	chemical quality profile of Polygoni Multiflori Radix at different processing degrees	15:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	12	27	used	used	2612:2615	arg2	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	27	used	used	2612:2615	arg2	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	27	used	used	2612:2615	arg2	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	27	used	used	2612:2615	arg2	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	0	28	theme	Radix	63:67	arg1	profile					32:38	chemical quality profile	15:38	chemical quality profile of Polygoni Multiflori Radix at different processing degrees	15:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	1	29	theme	Multiflori	332:341	arg1	Radix					343:347	Polygoni Multiflori Radix	323:347	Polygoni Multiflori Radix	323:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	9	30	theme	peaks	1542:1546	arg1	areas					1510:1514	the relative peak areas	1492:1514	the relative peak areas of the 26 chromatographic peaks in the sample	1492:1560	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	2	31	theme	pharmacopoeia	514:526	arg1	method					528:533	pharmacopoeia method	514:533	pharmacopoeia method	514:533	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	12	32	theme	processing	2642:2651	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	32	theme	processing	2642:2651	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	32	theme	processing	2642:2651	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	32	theme	processing	2642:2651	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	8	33	theme	stilbene	1409:1416	arg1	peak					1429:1432	peak 13	1429:1435	peak 13	1429:1435	It can be identified stilbene glycoside( peak 13),emodin( peak 21),and physcion( peak 24).
32237352	8	33	theme	stilbene	1409:1416	arg1	glycoside					1418:1426	stilbene glycoside	1409:1426	stilbene glycoside( peak 13)	1409:1436	It can be identified stilbene glycoside( peak 13),emodin( peak 21),and physcion( peak 24).
32237352	0	34	theme	different	72:80	arg1	degrees					93:99	different processing degrees	72:99	different processing degrees	72:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	11	35	theme	methyl	2296:2301	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	35	theme	methyl	2296:2301	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	35	theme	methyl	2296:2301	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	35	theme	methyl	2296:2301	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	12	36	dep	requirements	2456:2467	arg1	established					2469:2479	established	2469:2479	established a method of multi-component content determination combined with fingerprint	2469:2555	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	36	dep	requirements	2456:2467	arg1	clarified					2561:2569	clarified	2561:2569	clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process	2561:2809	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	9	37	from	products	1679:1686	arg1	areas					1644:1648	the relative peak areas	1626:1648	the relative peak areas of 12 peaks in the processed products	1626:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	37	from	products	1679:1686	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	38	theme	statistical	1758:1768	arg1	analysis					1770:1777	statistical analysis	1758:1777	statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees	1758:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	39	theme	determination	797:809	arg1	results					778:784	The results	774:784	The results of content determination	774:809	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	12	40	theme	nine-steaming	2770:2782	arg1	process					2803:2809	nine-steaming and ninesun-curing process	2770:2809	nine-steaming and ninesun-curing process	2770:2809	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	0	41	from	profile	32:38	arg1	degrees					93:99	different processing degrees	72:99	different processing degrees	72:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	11	42	theme	control	2320:2326	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	42	theme	control	2320:2326	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	42	theme	control	2320:2326	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	42	theme	control	2320:2326	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	2	43	theme	glycoside	390:398	arg1	polysaccharides					419:433	polysaccharides	419:433	polysaccharides	419:433	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	2	43	theme	glycoside	390:398	arg1	anthraquinones					400:413	anthraquinones	400:413	anthraquinones	400:413	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	2	43	theme	glycoside	390:398	arg1	contents					369:376	Their contents	363:376	Their contents of stilbene glycoside	363:398	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	7	44	theme	Fingerprint	1233:1243	arg1	results					1245:1251	Fingerprint results	1233:1251	Fingerprint results	1233:1251	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	5	45	theme	free	974:977	arg1	anthraquinone					979:991	the free anthraquinone	970:991	the free anthraquinone	970:991	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	11	46	theme	chemical	2059:2066	arg1	components					2068:2077	The eight chemical components	2049:2077	The eight chemical components	2049:2077	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	0	47	theme	chemical	15:22	arg1	profile					32:38	chemical quality profile	15:38	chemical quality profile of Polygoni Multiflori Radix at different processing degrees	15:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	5	48	theme	combined	917:924	arg1	anthraquinone					926:938	the combined anthraquinone	913:938	the combined anthraquinone	913:938	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	0	49	theme	profile	32:38	arg1	[Evaluation					0:10	[Evaluation	0:10	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees	0:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	1	50	theme	different	300:308	arg1	processes					310:318	different processes	300:318	different processes of Polygoni Multiflori Radix	300:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	2	51	theme	bean	499:502	arg1	juice					504:508	black bean juice	493:508	black bean juice	493:508	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	2	52	theme	black	493:497	arg1	juice					504:508	black bean juice	493:508	black bean juice	493:508	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	7	53	theme	different	1356:1364	arg1	processes					1377:1385	different processing processes	1356:1385	different processing processes	1356:1385	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	9	54	theme	processing	1829:1838	arg1	degrees					1840:1846	different processing degrees	1819:1846	different processing degrees	1819:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	11	55	from	difference	2123:2132	arg1	process					2175:2181	the process	2171:2181	the process of nine-steaming and nine-sun-curing	2171:2218	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	1	56	theme	nine-steaming	209:221	arg1	method					199:204	the ancient method	187:204	the ancient method of nine-steaming and nine-sun-curing	187:241	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	12	57	theme	multi-component	2493:2507	arg1	determination					2517:2529	multi-component content determination	2493:2529	multi-component content determination combined with fingerprint	2493:2555	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	1	58	theme	nine-sun-curing	227:241	arg1	method					199:204	the ancient method	187:204	the ancient method of nine-steaming and nine-sun-curing	187:241	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	11	59	theme	main	2088:2091	arg1	components					2093:2102	the main components	2084:2102	the main components	2084:2102	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	3	60	theme	HPLC	536:539	arg1	fingerprints					550:561	HPLC chemical fingerprints	536:561	HPLC chemical fingerprints	536:561	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	13	61	theme	processed	2890:2898	arg1	products					2900:2907	different processed products	2880:2907	different processed products of Polygoni Multiflori Radix	2880:2936	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	9	62	theme	peak	1639:1642	arg1	areas					1644:1648	the relative peak areas	1626:1648	the relative peak areas of 12 peaks in the processed products	1626:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	62	theme	peak	1639:1642	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	1	63	theme	composition	256:266	arg1	changes					268:274	the chemical composition changes	243:274	the chemical composition changes	243:274	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	13	64	theme	chemical	2840:2847	arg1	model					2868:2872	the chemical quality evaluation model	2836:2872	the chemical quality evaluation model	2836:2872	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	12	65	theme	chemical	2717:2724	arg1	composition					2726:2736	chemical composition	2717:2736	chemical composition	2717:2736	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	11	66	theme	nine-steaming	2186:2198	arg1	process					2175:2181	the process	2171:2181	the process of nine-steaming and nine-sun-curing	2171:2218	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	1	67	from	profiles	288:295	arg1	processes					310:318	different processes	300:318	different processes of Polygoni Multiflori Radix	300:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	5	68	from	trend	1049:1053	arg1	preparations					1072:1083	the different preparations	1058:1083	the different preparations of Polygoni Multiflori Radix and Pharmacopoeia	1058:1130	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	5	69	theme	stilbene	890:897	arg1	glycoside					899:907	the stilbene glycoside	886:907	the stilbene glycoside	886:907	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	13	70	theme	evaluation	2857:2866	arg1	model					2868:2872	the chemical quality evaluation model	2836:2872	the chemical quality evaluation model	2836:2872	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	9	71	theme	peaks	1656:1660	arg1	areas					1644:1648	the relative peak areas	1626:1648	the relative peak areas of 12 peaks in the processed products	1626:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	71	theme	peaks	1656:1660	arg1	higher					1693:1698	higher	1693:1698	higher	1693:1698	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	6	72	theme	classic	1215:1221	arg1	steaming					1223:1230	the classic steaming	1211:1230	the classic steaming	1211:1230	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	12	73	theme	methodological	2428:2441	arg1	requirements					2456:2467	the methodological verification requirements	2424:2467	the methodological verification requirements	2424:2467	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	7	74	theme	significant	1276:1286	arg1	differences					1288:1298	significant differences	1276:1298	significant differences in chemical composition in Polygoni Multiflori Radix	1276:1351	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	3	75	theme	squares-discriminant	609:628	arg1	OPLS-DA					640:646	OPLS-DA	640:646	OPLS-DA	640:646	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	3	75	theme	squares-discriminant	609:628	arg1	analysis					630:637	orthogonal partial least squares-discriminant analysis	584:637	orthogonal partial least squares-discriminant analysis( OPLS-DA)	584:647	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	12	76	theme	whole	2693:2697	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	76	theme	whole	2693:2697	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	76	theme	whole	2693:2697	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	76	theme	whole	2693:2697	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	9	77	from	areas	1510:1514	arg1	sample					1555:1560	the sample	1551:1560	the sample	1551:1560	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	78	theme	class	1904:1908	arg1	I					1910:1910	class I	1904:1910	class I	1904:1910	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	0	79	theme	processing	122:131	arg1	method					133:138	its classic processing method	110:138	its classic processing method	110:138	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	3	80	theme	partial	595:601	arg1	OPLS-DA					640:646	OPLS-DA	640:646	OPLS-DA	640:646	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	3	80	theme	partial	595:601	arg1	analysis					630:637	orthogonal partial least squares-discriminant analysis	584:637	orthogonal partial least squares-discriminant analysis( OPLS-DA)	584:647	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	5	81	theme	steaming	871:878	arg1	times					880:884	steaming times	871:884	steaming times	871:884	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	6	82	theme	finishing	1191:1199	arg1	products					1163:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	6	82	theme	finishing	1191:1199	arg1	point					1201:1205	the finishing point	1187:1205	the finishing point for the classic steaming	1187:1230	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	11	83	theme	nine-sun-curing	2204:2218	arg1	process					2175:2181	the process	2171:2181	the process of nine-steaming and nine-sun-curing	2171:2218	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	5	84	theme	Pharmacopoeia	1118:1130	arg1	preparations					1072:1083	the different preparations	1058:1083	the different preparations of Polygoni Multiflori Radix and Pharmacopoeia	1058:1130	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	85	theme	relative	1496:1503	arg1	areas					1510:1514	the relative peak areas	1492:1514	the relative peak areas of the 26 chromatographic peaks in the sample	1492:1560	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	86	from	areas	1644:1648	arg1	products					1679:1686	the processed products	1665:1686	the processed products	1665:1686	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	87	with	peaks	1962:1966	arg1	value					1981:1985	the VIP value	1973:1985	the VIP value higher than 1	1973:1999	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	88	theme	decreasing	949:958	arg1	trend					960:964	a decreasing trend	947:964	a decreasing trend	947:964	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	12	89	theme	improved	2623:2630	arg1	six-sun-curing					2593:2606	six-sun-curing	2593:2606	six-sun-curing	2593:2606	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	89	theme	improved	2623:2630	arg1	technology					2653:2662	an improved classical processing technology	2620:2662	an improved classical processing technology	2620:2662	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	89	theme	improved	2623:2630	arg1	six-steaming					2576:2587	six-steaming	2576:2587	six-steaming	2576:2587	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	12	89	theme	improved	2623:2630	arg1	change					2707:2712	the whole dynamic change	2689:2712	the whole dynamic change of chemical composition in Polygoni Multiflori Radix	2689:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	3	90	theme	different	666:674	arg1	products					686:693	different processed products	666:693	different processed products using SIMCA 14	666:708	HPLC chemical fingerprints were established,and orthogonal partial least squares-discriminant analysis( OPLS-DA) was performed on different processed products using SIMCA 14.
32237352	9	91	theme	products,13	1713:1723	arg1	peaks					1725:1729	the raw products,13 peaks	1705:1729	the raw products,13 peaks	1705:1729	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	7	92	from	differences	1288:1298	arg1	Radix					1347:1351	Radix	1347:1351	Radix	1347:1351	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	7	92	from	differences	1288:1298	arg1	composition					1312:1322	chemical composition	1303:1322	chemical composition	1303:1322	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	12	93	theme	ninesun-curing	2788:2801	arg1	process					2803:2809	nine-steaming and ninesun-curing process	2770:2809	nine-steaming and ninesun-curing process	2770:2809	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	9	94	with	class	1947:1951	arg1	value					1981:1985	the VIP value	1973:1985	the VIP value higher than 1	1973:1999	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	95	theme	Radix	1108:1112	arg1	preparations					1072:1083	the different preparations	1058:1083	the different preparations of Polygoni Multiflori Radix and Pharmacopoeia	1058:1130	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	6	96	used	used	1179:1182	arg2	point					1201:1205	the finishing point	1187:1205	the finishing point for the classic steaming	1187:1230	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	6	96	used	used	1179:1182	arg2	products					1163:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	9	97	theme	chromatographic	1526:1540	arg1	peaks					1542:1546	the 26 chromatographic peaks	1519:1546	the 26 chromatographic peaks	1519:1546	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	11	98	theme	Radix	2362:2366	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	98	theme	Radix	2362:2366	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	98	theme	Radix	2362:2366	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	98	theme	Radix	2362:2366	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	0	99	theme	processing	82:91	arg1	degrees					93:99	different processing degrees	72:99	different processing degrees	72:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	1	100	theme	Radix	343:347	arg1	processes					310:318	different processes	300:318	different processes of Polygoni Multiflori Radix	300:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	1	101	from	changes	268:274	arg1	processes					310:318	different processes	300:318	different processes of Polygoni Multiflori Radix	300:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	11	102	theme	emodin	2289:2294	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	102	theme	emodin	2289:2294	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	102	theme	emodin	2289:2294	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	102	theme	emodin	2289:2294	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	4	103	theme	quality	738:744	arg1	difference					746:755	the quality difference	734:755	the quality difference between samples	734:771	1 software to evaluate the quality difference between samples.
32237352	5	104	theme	total	993:997	arg1	anthraquinone					999:1011	total anthraquinone	993:1011	total anthraquinone	993:1011	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	105	from	degrees	1840:1846	arg1	analysis					1770:1777	statistical analysis	1758:1777	statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees	1758:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	106	theme	content	789:795	arg1	determination					797:809	content determination	789:809	content determination	789:809	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	1	107	theme	ancient	191:197	arg1	method					199:204	the ancient method	187:204	the ancient method of nine-steaming and nine-sun-curing	187:241	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	0	108	dep	based	101:105	arg1	"					174:174	nine-steaming and nine-sun-curing"	141:174	nine-steaming and nine-sun-curing"	141:174	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	6	109	theme	Six-steamed	1133:1143	arg1	products					1163:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products	1133:1170	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	6	109	theme	Six-steamed	1133:1143	arg1	point					1201:1205	the finishing point	1187:1205	the finishing point for the classic steaming	1187:1230	Six-steamed and six-sun-cured products can be used as the finishing point for the classic steaming.
32237352	11	110	theme	quality	2312:2318	arg1	indicators					2328:2337	quality control indicators	2312:2337	quality control indicators of Polygoni Multiflori Radix	2312:2366	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	110	theme	quality	2312:2318	arg1	glycoside					2268:2276	stilbene glycoside	2259:2276	stilbene glycoside	2259:2276	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	110	theme	quality	2312:2318	arg1	emodin					2278:2283	emodin	2278:2283	emodin	2278:2283	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	11	110	theme	quality	2312:2318	arg1	ether					2303:2307	emodin methyl ether	2289:2307	emodin methyl ether	2289:2307	The eight chemical components were the main components to distinguish the difference between Polygoni Multiflori Radix in the process of nine-steaming and nine-sun-curing,suggesting that it was rational to use stilbene glycoside,emodin and emodin methyl ether as quality control indicators of Polygoni Multiflori Radix.
32237352	5	111	theme	number	846:851	arg1	increase					830:837	the increase	826:837	the increase of the number of processing and steaming times	826:884	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	112	dep	reference	1587:1595	arg1	7					1605:1605	7	1605:1605	7	1605:1605	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	113	theme	VIP	1977:1979	arg1	value					1981:1985	the VIP value	1973:1985	the VIP value higher than 1	1973:1999	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	2	114	theme	stilbene	381:388	arg1	glycoside					390:398	stilbene glycoside	381:398	stilbene glycoside	381:398	Their contents of stilbene glycoside,anthraquinones and polysaccharides were determined by nine-steaming and nine-sun-curing with black bean juice and pharmacopoeia method.
32237352	0	115	theme	quality	24:30	arg1	profile					32:38	chemical quality profile	15:38	chemical quality profile of Polygoni Multiflori Radix at different processing degrees	15:99	[Evaluation of chemical quality profile of Polygoni Multiflori Radix at different processing degrees based on its classic processing method "nine-steaming and nine-sun-curing"].
32237352	1	116	theme	quality	280:286	arg1	profiles					288:295	quality profiles	280:295	quality profiles	280:295	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	9	117	theme	higher	1987:1992	arg1	value					1981:1985	the VIP value	1973:1985	the VIP value higher than 1	1973:1999	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	9	118	from	analysis	1770:1777	arg1	degrees					1840:1846	different processing degrees	1819:1846	different processing degrees	1819:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	7	119	theme	processing	1366:1375	arg1	processes					1377:1385	different processing processes	1356:1385	different processing processes	1356:1385	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	9	120	theme	Radix	1810:1814	arg1	analysis					1770:1777	statistical analysis	1758:1777	statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees	1758:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	5	121	theme	different	1062:1070	arg1	preparations					1072:1083	the different preparations	1058:1083	the different preparations of Polygoni Multiflori Radix and Pharmacopoeia	1058:1130	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	9	122	theme	different	1819:1827	arg1	degrees					1840:1846	different processing degrees	1819:1846	different processing degrees	1819:1846	By comparing the relative peak areas of the 26 chromatographic peaks in the sample after normalization( the reference is peak 7),it was found that the relative peak areas of 12 peaks in the processed products were higher than the raw products,13 peaks were reduced; according to statistical analysis of OPLS-DA,Polygoni Multiflori Radix at different processing degrees was further divided into three categories,sample S1 was class I,S2-S5 were class Ⅱ,and S6-S11 were class Ⅲ． And 8 peaks with the VIP value higher than 1.
32237352	1	123	theme	Polygoni	323:330	arg1	Radix					343:347	Polygoni Multiflori Radix	323:347	Polygoni Multiflori Radix	323:347	Based on the ancient method of nine-steaming and nine-sun-curing,the chemical composition changes and quality profiles in different processes of Polygoni Multiflori Radix were studied.
32237352	12	124	theme	determination	2517:2529	arg1	method					2483:2488	a method	2481:2488	a method of multi-component content determination combined with fingerprint	2481:2555	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
32237352	13	125	theme	Radix	2932:2936	arg1	products					2900:2907	different processed products	2880:2907	different processed products of Polygoni Multiflori Radix	2880:2936	It provides a basis for the chemical quality evaluation model about different processed products of Polygoni Multiflori Radix.
32237352	5	126	theme	upward	1042:1047	arg1	trend					1049:1053	an upward trend	1039:1053	an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia	1039:1130	The results of content determination show that,with the increase of the number of processing and steaming times,the stilbene glycoside and the combined anthraquinone showed a decreasing trend,and the free anthraquinone,total anthraquinone and polysaccharide showed an upward trend in the different preparations of Polygoni Multiflori Radix and Pharmacopoeia.
32237352	7	127	theme	chemical	1303:1310	arg1	composition					1312:1322	chemical composition	1303:1322	chemical composition	1303:1322	Fingerprint results showed that there were significant differences in chemical composition in Polygoni Multiflori Radix at different processing processes.
32237352	12	128	from	change	2707:2712	arg1	Radix					2761:2765	Radix	2761:2765	Radix	2761:2765	The method established in this experiment conformed to the methodological verification requirements,established a method of multi-component content determination combined with fingerprint,and clarified that six-steaming and six-sun-curing was used as an improved classical processing technology,and more clearly defined the whole dynamic change of chemical composition in Polygoni Multiflori Radix by nine-steaming and ninesun-curing process.
30783327	7	0	theme	Ho	1443:1444	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	0	theme	Ho	1443:1444	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	1	1	theme	HT-29	306:310	arg1	line					317:320	HT-29 cell line	306:320	HT-29 cell line	306:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	2	theme	purified	1425:1432	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	2	theme	purified	1425:1432	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	6	3	theme	IC	1159:1160	arg1	μg/ml					1172:1176	IC 50= 43.85 μg/ml	1159:1176	IC 50= 43.85 μg/ml	1159:1176	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	3	theme	IC	1159:1160	arg1	FrIV					1153:1156	purified fraction Ho FrIV	1132:1156	purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1132:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	4	theme	FrIV	1153:1156	arg1	activity					1120:1127	Noticeable anti-inflammatory activity	1091:1127	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1091:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	5	theme	fraction	1141:1148	arg1	μg/ml					1172:1176	IC 50= 43.85 μg/ml	1159:1176	IC 50= 43.85 μg/ml	1159:1176	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	5	theme	fraction	1141:1148	arg1	FrIV					1153:1156	purified fraction Ho FrIV	1132:1156	purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1132:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	1	6	from	line	317:320	arg1	properties					239:248	the anti-oxidant and anti-inflammatory properties	200:248	the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	200:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	5	7	theme	antioxidant	1039:1049	arg1	assay					1057:1061	ferric reducing antioxidant power assay	1023:1061	ferric reducing antioxidant power assay	1023:1061	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	6	8	theme	interleukin-8	1292:1304	arg1	secretion					1313:1321	the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion	1248:1321	the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml)	1248:1334	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	8	theme	interleukin-8	1292:1304	arg1	ng/ml					1329:1333	0.27 ng/ml	1324:1333	0.27 ng/ml	1324:1333	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	1	9	theme	seagrass	253:260	arg1	polysaccharide					288:301	seagrass Halophila ovalis sulfated polysaccharide	253:301	seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	253:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	10	theme	promising	1548:1556	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	10	theme	promising	1548:1556	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	11	theme	arabinose	853:861	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	11	theme	arabinose	853:861	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	1	12	from	polysaccharide	288:301	arg1	line					317:320	HT-29 cell line	306:320	HT-29 cell line	306:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	13	used	used	1538:1541	arg2	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	13	used	used	1538:1541	arg2	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	5	14	theme	ferric	1023:1028	arg1	assay					1057:1061	ferric reducing antioxidant power assay	1023:1061	ferric reducing antioxidant power assay	1023:1061	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	2	15	theme	transform-infrared	495:512	arg1	spectroscopy					522:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	2	16	dep	SUBJECTS	323:330	arg1	Monosaccharides					344:358	Monosaccharides	344:358	Monosaccharides	344:358	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	6	17	theme	HT-29	1339:1343	arg1	line					1350:1353	HT-29 cell line	1339:1353	HT-29 cell line	1339:1353	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	0	18	theme	chemokine	97:105	arg1	interleukin-8					107:119	tumor necrosis factor-α-induced chemokine interleukin-8	65:119	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	1	19	dep	OBJECTIVES	151:160	arg1	aims					180:183	aims	180:183	aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	180:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	20	theme	IL-8	1506:1509	arg1	secretion					1493:1501	the TNF-α-induced secretion	1475:1501	the TNF-α-induced secretion of IL-8 in HT-29	1475:1518	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	0	21	theme	HT-29	134:138	arg1	line					145:148	HT-29 cell line	134:148	HT-29 cell line	134:148	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	0	22	theme	factor-α-induced	80:95	arg1	interleukin-8					107:119	tumor necrosis factor-α-induced chemokine interleukin-8	65:119	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	2	23	theme	functional	449:458	arg1	groups					460:465	the functional groups	445:465	the functional groups	445:465	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	0	24	theme	Sulfated	0:7	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides of seagrass Halophila ovalis	0:52	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	2	25	theme	FT-IR	515:519	arg1	spectroscopy					522:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	6	26	located	observed	1183:1190	arg2	activity					1120:1127	Noticeable anti-inflammatory activity	1091:1127	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1091:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	26	located	observed	1183:1190	arg1	range					1210:1214	a noncytotoxic range	1195:1214	a noncytotoxic range of concentrations	1195:1232	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	3	27	theme	antioxidant	540:550	arg1	potential					574:582	The antioxidant and anti-inflammatory potential	536:582	The antioxidant and anti-inflammatory potential of crude extract and purified fractions	536:622	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	7	28	theme	TNF-α-induced	1479:1491	arg1	secretion					1493:1501	the TNF-α-induced secretion	1475:1501	the TNF-α-induced secretion of IL-8 in HT-29	1475:1518	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	29	theme	FT-IR	659:663	arg1	spectra					665:671	RESULTS FT-IR spectra	651:671	RESULTS FT-IR spectra	651:671	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	0	30	theme	Halophila	37:45	arg1	ovalis					47:52	seagrass Halophila ovalis	28:52	seagrass Halophila ovalis	28:52	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	0	31	theme	necrosis	71:78	arg1	interleukin-8					107:119	tumor necrosis factor-α-induced chemokine interleukin-8	65:119	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	7	32	theme	Ho-SP	1454:1458	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	32	theme	Ho-SP	1454:1458	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	3	33	theme	anti-inflammatory	556:572	arg1	potential					574:582	The antioxidant and anti-inflammatory potential	536:582	The antioxidant and anti-inflammatory potential of crude extract and purified fractions	536:622	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	1	34	theme	Halophila	262:270	arg1	polysaccharide					288:301	seagrass Halophila ovalis sulfated polysaccharide	253:301	seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	253:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	5	35	theme	scavenging	935:944	arg1	activity					946:953	radical scavenging activity	927:953	radical scavenging activity	927:953	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	6	36	theme	Noticeable	1091:1100	arg1	activity					1120:1127	Noticeable anti-inflammatory activity	1091:1127	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1091:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	3	37	theme	purified	605:612	arg1	fractions					614:622	purified fractions	605:622	purified fractions	605:622	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	1	38	theme	sulfated	279:286	arg1	polysaccharide					288:301	seagrass Halophila ovalis sulfated polysaccharide	253:301	seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	253:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	39	theme	fraction	1434:1441	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	39	theme	fraction	1434:1441	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	40	theme	functional	713:722	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	40	theme	functional	713:722	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	3	41	theme	extract	593:599	arg1	potential					574:582	The antioxidant and anti-inflammatory potential	536:582	The antioxidant and anti-inflammatory potential of crude extract and purified fractions	536:622	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	2	42	theme	METHODS	336:342	arg1	composition					360:370	SUBJECTS AND METHODS Monosaccharides composition	323:370	SUBJECTS AND METHODS Monosaccharides composition	323:370	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	1	43	theme	cell	312:315	arg1	line					317:320	HT-29 cell line	306:320	HT-29 cell line	306:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	6	44	theme	50=	1162:1164	arg1	μg/ml					1172:1176	IC 50= 43.85 μg/ml	1159:1176	IC 50= 43.85 μg/ml	1159:1176	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	44	theme	50=	1162:1164	arg1	FrIV					1153:1156	purified fraction Ho FrIV	1132:1156	purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1132:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	2	45	theme	SUBJECTS	323:330	arg1	composition					360:370	SUBJECTS AND METHODS Monosaccharides composition	323:370	SUBJECTS AND METHODS Monosaccharides composition	323:370	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	7	46	from	secretion	1493:1501	arg1	HT-29					1514:1518	HT-29	1514:1518	HT-29	1514:1518	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	47	theme	galactose	751:759	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	47	theme	galactose	751:759	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	6	48	theme	concentrations	1219:1232	arg1	range					1210:1214	a noncytotoxic range	1195:1214	a noncytotoxic range of concentrations	1195:1232	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	1	49	theme	anti-oxidant	204:215	arg1	properties					239:248	the anti-oxidant and anti-inflammatory properties	200:248	the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	200:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	50	theme	anti-inflammatory	1574:1590	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	50	theme	anti-inflammatory	1574:1590	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	51	theme	glucose	833:839	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	51	theme	glucose	833:839	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	6	52	theme	Ho	1150:1151	arg1	μg/ml					1172:1176	IC 50= 43.85 μg/ml	1159:1176	IC 50= 43.85 μg/ml	1159:1176	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	52	theme	Ho	1150:1151	arg1	FrIV					1153:1156	purified fraction Ho FrIV	1132:1156	purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1132:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	1	53	theme	anti-inflammatory	221:237	arg1	properties					239:248	the anti-oxidant and anti-inflammatory properties	200:248	the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	200:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	7	54	theme	antioxidant	1558:1568	arg1	FrIV					1446:1449	purified fraction Ho FrIV	1425:1449	purified fraction Ho FrIV of Ho-SP	1425:1458	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	7	54	theme	antioxidant	1558:1568	arg1	candidate					1592:1600	a promising antioxidant and anti-inflammatory candidate	1546:1600	a promising antioxidant and anti-inflammatory candidate with potential benefits	1546:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	6	55	theme	purified	1132:1139	arg1	μg/ml					1172:1176	IC 50= 43.85 μg/ml	1159:1176	IC 50= 43.85 μg/ml	1159:1176	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	55	theme	purified	1132:1139	arg1	FrIV					1153:1156	purified fraction Ho FrIV	1132:1156	purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1132:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	2	56	theme	chromatography-mass	400:418	arg1	LC-MS					434:438	LC-MS	434:438	LC-MS	434:438	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	2	56	theme	chromatography-mass	400:418	arg1	spectrometry					420:431	liquid chromatography-mass spectrometry	393:431	liquid chromatography-mass spectrometry (LC-MS)	393:439	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	5	57	theme	power	1051:1055	arg1	assay					1057:1061	ferric reducing antioxidant power assay	1023:1061	ferric reducing antioxidant power assay	1023:1061	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	6	58	theme	noncytotoxic	1197:1208	arg1	range					1210:1214	a noncytotoxic range	1195:1214	a noncytotoxic range of concentrations	1195:1232	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	4	59	theme	presence	739:746	arg1	presence					691:698	the presence	687:698	the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%)	687:868	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	6	60	theme	-induced	1283:1290	arg1	IL-8					1307:1310	IL-8	1307:1310	IL-8	1307:1310	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	6	60	theme	-induced	1283:1290	arg1	interleukin-8					1292:1304	the tumor necrosis factor-α (TNF-α)-induced interleukin-8	1248:1304	the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml)	1248:1334	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	1	61	from	properties	239:248	arg1	line					317:320	HT-29 cell line	306:320	HT-29 cell line	306:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	2	62	theme	liquid	393:398	arg1	LC-MS					434:438	LC-MS	434:438	LC-MS	434:438	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	2	62	theme	liquid	393:398	arg1	spectrometry					420:431	liquid chromatography-mass spectrometry	393:431	liquid chromatography-mass spectrometry (LC-MS)	393:439	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	6	63	theme	cell	1345:1348	arg1	line					1350:1353	HT-29 cell line	1339:1353	HT-29 cell line	1339:1353	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	5	64	theme	reducing	1030:1037	arg1	assay					1057:1061	ferric reducing antioxidant power assay	1023:1061	ferric reducing antioxidant power assay	1023:1061	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	1	65	theme	present	166:172	arg1	study					174:178	The present study	162:178	The present study	162:178	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	0	66	theme	interleukin-8	107:119	arg1	secretion					121:129	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	7	67	dep	CONCLUSION	1356:1365	arg1	suggest					1412:1418	suggest	1412:1418	suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits	1412:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	68	theme	xylose	770:775	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	68	theme	xylose	770:775	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	0	69	theme	cell	140:143	arg1	line					145:148	HT-29 cell line	134:148	HT-29 cell line	134:148	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	5	70	theme	dose-dependent	1068:1081	arg1	manner					1083:1088	a dose-dependent manner	1066:1088	a dose-dependent manner	1066:1088	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	4	71	theme	mannose	802:808	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	71	theme	mannose	802:808	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	72	theme	RESULTS	651:657	arg1	spectra					665:671	RESULTS FT-IR spectra	651:671	RESULTS FT-IR spectra	651:671	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	73	theme	fructose	785:792	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	0	74	theme	seagrass	28:35	arg1	ovalis					47:52	seagrass Halophila ovalis	28:52	seagrass Halophila ovalis	28:52	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	7	75	with	candidate	1592:1600	arg1	benefits					1617:1624	potential benefits	1607:1624	potential benefits	1607:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	2	76	theme	Fourier	487:493	arg1	spectroscopy					522:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	Fourier transform-infrared (FT-IR) spectroscopy	487:533	SUBJECTS AND METHODS Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy.
30783327	0	77	theme	ovalis	47:52	arg1	polysaccharides					9:23	Sulfated polysaccharides	0:23	Sulfated polysaccharides of seagrass Halophila ovalis	0:52	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	7	78	theme	potential	1607:1615	arg1	benefits					1617:1624	potential benefits	1607:1624	potential benefits	1607:1624	CONCLUSION Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.
30783327	4	79	theme	fucose	818:823	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	presence					739:746	the presence	735:746	the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose	735:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	79	theme	fucose	818:823	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	0	80	theme	tumor	65:69	arg1	interleukin-8					107:119	tumor necrosis factor-α-induced chemokine interleukin-8	65:119	tumor necrosis factor-α-induced chemokine interleukin-8 secretion	65:129	Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.
30783327	5	81	theme	2-azinobis-3-ethylbenzothiazoline-6-sulfonic	968:1011	arg1	acid					1013:1016	2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid	968:1016	2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid	968:1016	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	3	82	theme	crude	587:591	arg1	extract					593:599	crude extract	587:599	crude extract	587:599	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	5	83	theme	radical	927:933	arg1	activity					946:953	radical scavenging activity	927:953	radical scavenging activity	927:953	Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner.
30783327	6	84	theme	anti-inflammatory	1102:1118	arg1	activity					1120:1127	Noticeable anti-inflammatory activity	1091:1127	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml)	1091:1177	Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line.
30783327	4	85	theme	different	703:711	arg1	%					781:781	7.6%	778:781	7.6%	778:781	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	glucose					833:839	glucose	833:839	glucose (1.2%)	833:846	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					829:829	1.6%	826:829	1.6%	826:829	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	mannose					802:808	mannose	802:808	mannose (2.0%)	802:815	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	arabinose					853:861	arabinose	853:861	arabinose	853:861	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	fucose					818:823	fucose	818:823	fucose (1.6%)	818:830	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	xylose					770:775	xylose	770:775	xylose (7.6%)	770:782	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	fructose					785:792	fructose	785:792	fructose (4.0%)	785:799	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	galactose					751:759	galactose	751:759	galactose (82.4%)	751:767	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	groups					724:729	different functional groups	703:729	different functional groups	703:729	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					845:845	1.2%	842:845	1.2%	842:845	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					798:798	4.0%	795:798	4.0%	795:798	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					814:814	2.0%	811:814	2.0%	811:814	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					867:867	1.0%	864:867	1.0%	864:867	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	4	85	theme	different	703:711	arg1	%					766:766	82.4%	762:766	82.4%	762:766	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	1	86	theme	ovalis	272:277	arg1	polysaccharide					288:301	seagrass Halophila ovalis sulfated polysaccharide	253:301	seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	253:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
30783327	4	87	theme	groups	724:729	arg1	presence					691:698	the presence	687:698	the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%)	687:868	RESULTS FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS.
30783327	3	88	theme	fractions	614:622	arg1	potential					574:582	The antioxidant and anti-inflammatory potential	536:582	The antioxidant and anti-inflammatory potential of crude extract and purified fractions	536:622	The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro.
30783327	1	89	theme	polysaccharide	288:301	arg1	properties					239:248	the anti-oxidant and anti-inflammatory properties	200:248	the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line	200:320	OBJECTIVES The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line.
31361180	3	0	theme	insoluble	360:368	arg1	sp					389:390	a soluble or insoluble form.Lactobacillus sp	347:390	a soluble or insoluble form.Lactobacillus sp	347:390	These polysaccharides can be secreted from the microbes either in a soluble or insoluble form.Lactobacillus sp.
31361180	9	1	theme	triammonium	1271:1281	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	1	theme	triammonium	1271:1281	arg1	citrate					1283:1289	triammonium citrate 1.5	1271:1293	triammonium citrate 1.5	1271:1293	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	2	theme	dihydrogen	1245:1254	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	2	theme	dihydrogen	1245:1254	arg1	phosphate					1256:1264	potassium dihydrogen phosphate 2.5	1235:1268	potassium dihydrogen phosphate 2.5	1235:1268	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	10	3	theme	experiments	1354:1364	arg1	sets					1342:1345	32 sets	1339:1345	32 sets of RSM experiments	1339:1364	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	10	3	theme	experiments	1354:1364	arg1	experiments					1354:1364	RSM experiments	1350:1364	RSM experiments	1350:1364	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	6	4	theme	aspects	659:665	arg1	one					642:644	one	642:644	one	642:644	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	6	4	theme	aspects	659:665	arg1	aspects					659:665	the major aspects	649:665	the major aspects for the production of EPS from Lactobacillus sp.	649:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	11	5	theme	Levenberg-Marquardt	1426:1444	arg1	LMA					1457:1459	LMA	1457:1459	LMA	1457:1459	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	5	theme	Levenberg-Marquardt	1426:1444	arg1	Algorithm					1446:1454	Levenberg-Marquardt Algorithm	1426:1454	Levenberg-Marquardt Algorithm (LMA)	1426:1460	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	10	6	theme	sets	1342:1345	arg1	output					1329:1334	The output	1325:1334	The output of 32 sets of RSM experiments	1325:1364	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	10	7	theme	RSM	1350:1352	arg1	experiments					1354:1364	RSM experiments	1350:1364	RSM experiments	1350:1364	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	6	8	theme	major	653:657	arg1	aspects					659:665	the major aspects	649:665	the major aspects for the production of EPS from Lactobacillus sp.	649:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	0	9	from	sp	68:69	arg1	optimization					15:26	optimization	15:26	optimization	15:26	Extraction and optimization of exopolysaccharide from Lactobacillus sp.
31361180	0	9	from	sp	68:69	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and optimization of exopolysaccharide from Lactobacillus sp.
31361180	7	10	theme	artificial	973:982	arg1	ANN					1001:1003	ANN	1001:1003	ANN	1001:1003	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	7	10	theme	artificial	973:982	arg1	networks					991:998	artificial neural networks	973:998	artificial neural networks (ANN)	973:1004	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	9	11	theme	production	1111:1120	arg1	yeild					1098:1102	The highest yeild	1086:1102	The highest yeild of EPS production in RSM	1086:1127	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	11	12	theme	Gradient	1524:1531	arg1	SCGA					1544:1547	SCGA	1544:1547	SCGA	1544:1547	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	12	theme	Gradient	1524:1531	arg1	Algorithm					1533:1541	Scaled Conjugate Gradient Algorithm	1507:1541	Scaled Conjugate Gradient Algorithm (SCGA) among them	1507:1559	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	10	13	with	fitting	1382:1388	arg1	ANN					1395:1397	ANN	1395:1397	ANN	1395:1397	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	10	13	with	fitting	1382:1388	arg1	algorithms					1410:1419	three algorithms	1404:1419	three algorithms viz	1404:1423	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	7	14	theme	neural	984:989	arg1	ANN					1001:1003	ANN	1001:1003	ANN	1001:1003	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	7	14	theme	neural	984:989	arg1	networks					991:998	artificial neural networks	973:998	artificial neural networks (ANN)	973:1004	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	1	15	theme	response	78:85	arg1	methodology					95:105	response surface methodology	78:105	response surface methodology	78:105	using response surface methodology and artificial neural networks.
31361180	6	16	theme	components	740:749	arg1	optimization					717:728	optimization	717:728	optimization of medium components	717:749	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	8	17	theme	highest	1064:1070	arg1	yeild					1072:1076	the highest yeild	1060:1076	the highest yeild of EPS	1060:1083	Three algorithms of ANN were compared to investigate the highest yeild of EPS.
31361180	4	18	theme	organisms	407:415	arg1	one					396:398	one	396:398	one	396:398	is one of the organisms that have been found to produce exopolysaccharide.
31361180	4	18	theme	organisms	407:415	arg1	organisms					407:415	the organisms	403:415	the organisms	403:415	is one of the organisms that have been found to produce exopolysaccharide.
31361180	1	19	theme	surface	87:93	arg1	methodology					95:105	response surface methodology	78:105	response surface methodology	78:105	using response surface methodology and artificial neural networks.
31361180	5	20	theme	antimicrobial	543:555	arg1	activity					557:564	antimicrobial activity	543:564	antimicrobial activity	543:564	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	3	21	dep	microbes	328:335	arg1	either					337:342	either	337:342	either	337:342	These polysaccharides can be secreted from the microbes either in a soluble or insoluble form.Lactobacillus sp.
31361180	8	22	theme	EPS	1081:1083	arg1	yeild					1072:1076	the highest yeild	1060:1076	the highest yeild of EPS	1060:1083	Three algorithms of ANN were compared to investigate the highest yeild of EPS.
31361180	6	23	from	sp.	712:714	arg1	EPS					689:691	EPS	689:691	EPS from Lactobacillus sp.	689:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	6	23	from	sp.	712:714	arg1	production					675:684	the production	671:684	the production of EPS from Lactobacillus sp.	671:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	9	24	theme	highest	1090:1096	arg1	yeild					1098:1102	The highest yeild	1086:1102	The highest yeild of EPS production in RSM	1086:1127	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	10	25	dep	algorithms	1410:1419	arg1	viz					1421:1423	viz	1421:1423	three algorithms viz	1404:1423	The output of 32 sets of RSM experiments were tested for fitting with ANN with three algorithms viz.
31361180	9	26	theme	magnesium	1301:1309	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	26	theme	magnesium	1301:1309	arg1	sulfate					1311:1317	magnesium sulfate 0.25	1301:1322	magnesium sulfate 0.25	1301:1322	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	6	27	theme	medium	733:738	arg1	components					740:749	medium components	733:749	medium components	733:749	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	7	28	with	production	819:828	arg1	composition					873:883	different medium composition	856:883	different medium composition	856:883	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	11	29	theme	Scaled	1507:1512	arg1	SCGA					1544:1547	SCGA	1544:1547	SCGA	1544:1547	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	29	theme	Scaled	1507:1512	arg1	Algorithm					1533:1541	Scaled Conjugate Gradient Algorithm	1507:1541	Scaled Conjugate Gradient Algorithm (SCGA) among them	1507:1559	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	7	30	theme	different	856:864	arg1	composition					873:883	different medium composition	856:883	different medium composition	856:883	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	9	31	theme	dihydrogen	1211:1220	arg1	phosphate					1222:1230	sodium dihydrogen phosphate 3	1204:1232	sodium dihydrogen phosphate 3	1204:1232	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	31	theme	dihydrogen	1211:1220	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	2	32	dep	polysaccharides	153:167	arg1	secreted					169:176	secreted	169:176	secreted	169:176	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	2	32	dep	polysaccharides	153:167	arg1	produced					182:189	produced	182:189	produced from various microbes into their extracellular environment	182:248	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	8	33	theme	ANN	1027:1029	arg1	algorithms					1013:1022	Three algorithms	1007:1022	Three algorithms of ANN	1007:1029	Three algorithms of ANN were compared to investigate the highest yeild of EPS.
31361180	1	34	theme	artificial	111:120	arg1	networks					129:136	artificial neural networks	111:136	artificial neural networks	111:136	using response surface methodology and artificial neural networks.
31361180	7	35	theme	medium	866:871	arg1	composition					873:883	different medium composition	856:883	different medium composition	856:883	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	11	36	with	fit	1584:1586	arg1	experiments					1597:1607	the experiments	1593:1607	the experiments	1593:1607	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	9	37	theme	medium	1149:1154	arg1	composition					1156:1166	the medium composition	1145:1166	the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25	1145:1322	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	38	theme	sodium	1204:1209	arg1	phosphate					1222:1230	sodium dihydrogen phosphate 3	1204:1232	sodium dihydrogen phosphate 3	1204:1232	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	38	theme	sodium	1204:1209	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	1	39	theme	neural	122:127	arg1	networks					129:136	artificial neural networks	111:136	artificial neural networks	111:136	using response surface methodology and artificial neural networks.
31361180	7	40	theme	exopolysaccharide	833:849	arg1	production					819:828	the production	815:828	the production of exopolysaccharide with different medium composition	815:883	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	7	41	theme	response	902:909	arg1	methodology					919:929	response surface methodology	902:929	response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN)	902:1004	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	7	41	theme	response	902:909	arg1	RSM					932:934	RSM	932:934	RSM	932:934	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	5	42	theme	drug	528:531	arg1	delivery					533:540	drug delivery	528:540	drug delivery	528:540	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	0	43	theme	exopolysaccharide	31:47	arg1	optimization					15:26	optimization	15:26	optimization	15:26	Extraction and optimization of exopolysaccharide from Lactobacillus sp.
31361180	0	43	theme	exopolysaccharide	31:47	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and optimization of exopolysaccharide from Lactobacillus sp.
31361180	7	44	theme	present	801:807	arg1	work					809:812	the present work	797:812	the present work	797:812	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	6	45	theme	Medium	620:625	arg1	composition					627:637	Medium composition	620:637	Medium composition	620:637	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	2	46	theme	various	196:202	arg1	microbes					204:211	various microbes	196:211	various microbes into their extracellular environment	196:248	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	5	47	contain	have	494:497	arg2	applications					507:518	various applications	499:518	various applications such as drug delivery, antimicrobial activity, surgical implants and many more	499:597	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg1	Exo-polysaccharides					468:486	Exo-polysaccharides	468:486	Exo-polysaccharides (EPS)	468:492	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg2	delivery					533:540	drug delivery	528:540	drug delivery	528:540	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg2	implants					576:583	surgical implants	567:583	surgical implants	567:583	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg2	activity					557:564	antimicrobial activity	543:564	antimicrobial activity	543:564	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg2	many					589:592	many	589:592	many	589:592	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	47	contain	have	494:497	arg1	EPS					489:491	EPS	489:491	EPS	489:491	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	6	48	dep	one	642:644	arg1	help					755:758	help	755:758	can help to enhance the synthesis of EPS	751:790	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	6	49	theme	EPS	788:790	arg1	synthesis					775:783	the synthesis	771:783	the synthesis of EPS	771:790	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	0	50	theme	Lactobacillus	54:66	arg1	sp					68:69	Lactobacillus sp	54:69	Lactobacillus sp	54:69	Extraction and optimization of exopolysaccharide from Lactobacillus sp.
31361180	3	51	theme	soluble	349:355	arg1	sp					389:390	a soluble or insoluble form.Lactobacillus sp	347:390	a soluble or insoluble form.Lactobacillus sp	347:390	These polysaccharides can be secreted from the microbes either in a soluble or insoluble form.Lactobacillus sp.
31361180	11	52	theme	Conjugate	1514:1522	arg1	SCGA					1544:1547	SCGA	1544:1547	SCGA	1544:1547	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	52	theme	Conjugate	1514:1522	arg1	Algorithm					1533:1541	Scaled Conjugate Gradient Algorithm	1507:1541	Scaled Conjugate Gradient Algorithm (SCGA) among them	1507:1559	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	9	53	theme	potassium	1235:1243	arg1	dextrose					1191:1198	dextrose 15	1191:1201	dextrose 15	1191:1201	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	9	53	theme	potassium	1235:1243	arg1	phosphate					1256:1264	potassium dihydrogen phosphate 2.5	1235:1268	potassium dihydrogen phosphate 2.5	1235:1268	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	5	54	theme	different	602:610	arg1	fields					612:617	different fields	602:617	different fields	602:617	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	6	55	theme	EPS	689:691	arg1	production					675:684	the production	671:684	the production of EPS from Lactobacillus sp.	671:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	2	56	theme	extracellular	224:236	arg1	environment					238:248	their extracellular environment	218:248	their extracellular environment	218:248	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	9	57	from	yeild	1098:1102	arg1	RSM					1125:1127	RSM	1125:1127	RSM	1125:1127	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	6	58	from	production	675:684	arg1	sp.					712:714	Lactobacillus sp.	698:714	Lactobacillus sp.	698:714	Medium composition is one of the major aspects for the production of EPS from Lactobacillus sp., optimization of medium components can help to enhance the synthesis of EPS .
31361180	9	59	theme	EPS	1107:1109	arg1	production					1111:1120	EPS production	1107:1120	EPS production	1107:1120	The highest yeild of EPS production in RSM was achieved by the medium composition that consists of (g/L) dextrose 15, sodium dihydrogen phosphate 3, potassium dihydrogen phosphate 2.5, triammonium citrate 1.5, and, magnesium sulfate 0.25.
31361180	5	60	dep	many	589:592	arg1	more					594:597	more	594:597	more	594:597	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	3	61	theme	form.Lactobacillus	370:387	arg1	sp					389:390	a soluble or insoluble form.Lactobacillus sp	347:390	a soluble or insoluble form.Lactobacillus sp	347:390	These polysaccharides can be secreted from the microbes either in a soluble or insoluble form.Lactobacillus sp.
31361180	7	62	theme	surface	911:917	arg1	methodology					919:929	response surface methodology	902:929	response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN)	902:1004	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	7	62	theme	surface	911:917	arg1	RSM					932:934	RSM	932:934	RSM	932:934	In the present work, the production of exopolysaccharide with different medium composition was optimized by response surface methodology (RSM) followed by tested for fitting with artificial neural networks (ANN).
31361180	11	63	theme	Bayesian	1463:1470	arg1	BRA					1498:1500	BRA	1498:1500	BRA	1498:1500	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	63	theme	Bayesian	1463:1470	arg1	Algorithm					1487:1495	Bayesian Regularization Algorithm	1463:1495	Bayesian Regularization Algorithm (BRA)	1463:1501	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	5	64	theme	surgical	567:574	arg1	implants					576:583	surgical implants	567:583	surgical implants	567:583	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	65	theme	various	499:505	arg1	implants					576:583	surgical implants	567:583	surgical implants	567:583	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	65	theme	various	499:505	arg1	applications					507:518	various applications	499:518	various applications such as drug delivery, antimicrobial activity, surgical implants and many more	499:597	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	65	theme	various	499:505	arg1	delivery					533:540	drug delivery	528:540	drug delivery	528:540	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	65	theme	various	499:505	arg1	many					589:592	many	589:592	many	589:592	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	5	65	theme	various	499:505	arg1	activity					557:564	antimicrobial activity	543:564	antimicrobial activity	543:564	Exo-polysaccharides (EPS) have various applications such as drug delivery, antimicrobial activity, surgical implants and many more in different fields.
31361180	11	66	dep	SCGA	1628:1631	arg1	the					1624:1626	the	1624:1626	the	1624:1626	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	67	dep	Algorithm	1446:1454	arg1	LMA					1561:1563	LMA	1561:1563	LMA	1561:1563	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	2	68	theme	microbial	143:151	arg1	exopolysaccharide					262:278	exopolysaccharide	262:278	exopolysaccharide	262:278	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	2	68	theme	microbial	143:151	arg1	polysaccharides					153:167	The microbial polysaccharides	139:167	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment	139:248	The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide.
31361180	11	69	theme	Regularization	1472:1485	arg1	BRA					1498:1500	BRA	1498:1500	BRA	1498:1500	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
31361180	11	69	theme	Regularization	1472:1485	arg1	Algorithm					1487:1495	Bayesian Regularization Algorithm	1463:1495	Bayesian Regularization Algorithm (BRA)	1463:1501	Levenberg-Marquardt Algorithm (LMA), Bayesian Regularization Algorithm (BRA) and Scaled Conjugate Gradient Algorithm (SCGA) among them LMA found to have best fit with the experiments as compared to the SCGA and BRA.
29960906	0	0	theme	trace-level	110:120	arg1	detection					122:130	trace-level detection	110:130	trace-level detection of antibiotic drug metronidazole	110:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	2	1	theme	CuO	520:522	arg1	NPs					524:526	the CuO NPs	516:526	the CuO NPs	516:526	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	5	2	theme	Cyclic	831:836	arg1	results					850:856	Cyclic voltammetry results	831:856	Cyclic voltammetry results	831:856	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	9	3	from	sensitive	1504:1512	arg1	range					1558:1562	a range	1556:1562	a range of potentially interfering electroactive compounds	1556:1613	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	3	from	sensitive	1504:1512	arg1	addition					1473:1480	addition	1473:1480	addition	1473:1480	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	2	4	theme	oxide	382:386	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	5	5	theme	voltammetry	838:848	arg1	results					850:856	Cyclic voltammetry results	831:856	Cyclic voltammetry results	831:856	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	2	6	theme	copper	375:380	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	8	7	theme	reported	1448:1455	arg1	sensors					1461:1467	the previously reported MTZ sensors	1433:1467	the previously reported MTZ sensors	1433:1467	This sensor showed the lowest limit of detection of 0.6 nM and was much lower than the previously reported MTZ sensors.
29960906	2	8	theme	composite	560:568	arg1	electrodes					579:588	GR-β-CD composite modified electrodes	552:588	GR-β-CD composite modified electrodes	552:588	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	5	9	dep	NPs	886:888	arg1	electrode					909:917	composite modified electrode	890:917	the GR-β-CD/CuO NPs composite modified electrode	870:917	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	10	theme	other	1056:1060	arg1	GR-β-CD					1096:1102	GR-β-CD	1096:1102	GR-β-CD	1096:1102	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	10	theme	other	1056:1060	arg1	NPs					1113:1115	CuO NPs	1109:1115	CuO NPs	1109:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	10	theme	other	1056:1060	arg1	GR					1092:1093	GR	1092:1093	GR	1092:1093	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	10	theme	other	1056:1060	arg1	electrodes					1071:1080	other modified electrodes	1056:1080	other modified electrodes including GR, GR-β-CD, and CuO NPs	1056:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	6	11	theme	modified	1228:1235	arg1	electrodes					1237:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	1	12	theme	fields	287:292	arg1	electroanalysis					304:318	electroanalysis	304:318	electroanalysis of organic chemicals	304:339	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	1	12	theme	fields	287:292	arg1	range					278:282	the range	274:282	the range of fields including electroanalysis of organic chemicals	274:339	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	2	13	theme	GR-β-CD	552:558	arg1	electrodes					579:588	GR-β-CD composite modified electrodes	552:588	GR-β-CD composite modified electrodes	552:588	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	5	14	theme	modified	1062:1069	arg1	GR-β-CD					1096:1102	GR-β-CD	1096:1102	GR-β-CD	1096:1102	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	14	theme	modified	1062:1069	arg1	NPs					1113:1115	CuO NPs	1109:1115	CuO NPs	1109:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	14	theme	modified	1062:1069	arg1	GR					1092:1093	GR	1092:1093	GR	1092:1093	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	14	theme	modified	1062:1069	arg1	electrodes					1071:1080	other modified electrodes	1056:1080	other modified electrodes including GR, GR-β-CD, and CuO NPs	1056:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	4	15	theme	scanning	758:765	arg1	microscopy					776:785	the high-resolution scanning electron microscopy	738:785	the high-resolution scanning electron microscopy	738:785	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	5	16	theme	metronidazole	1031:1043	arg1	detection					1018:1026	the electrochemical detection	998:1026	the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs	998:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	0	17	theme	drug	146:149	arg1	metronidazole					151:163	antibiotic drug metronidazole	135:163	antibiotic drug metronidazole	135:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	6	18	theme	composite	1218:1226	arg1	electrodes					1237:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	2	19	theme	GR-β-CD	446:452	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	2	20	theme	NPs	406:408	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	2	21	theme	decorated	411:419	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	0	22	theme	antibiotic	135:144	arg1	metronidazole					151:163	antibiotic drug metronidazole	135:163	antibiotic drug metronidazole	135:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	5	23	theme	GR-β-CD/CuO	874:884	arg1	NPs					886:888	the GR-β-CD/CuO NPs	870:888	the GR-β-CD/CuO NPs composite modified electrode	870:917	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	8	24	theme	lowest	1373:1378	arg1	limit					1380:1384	the lowest limit	1369:1384	the lowest limit of detection of 0.6 nM	1369:1407	This sensor showed the lowest limit of detection of 0.6 nM and was much lower than the previously reported MTZ sensors.
29960906	4	25	theme	NPs	701:703	arg1	composites					705:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	0	26	theme	electrochemical	6:20	arg1	synthesis					22:30	Novel electrochemical synthesis	0:30	Novel electrochemical synthesis of copper oxide	0:46	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	9	27	from	addition	1473:1480	arg1	sensor					1487:1492	the sensor	1483:1492	the sensor	1483:1492	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	27	from	addition	1473:1480	arg1	sensitive					1504:1512	sensitive	1504:1512	sensitive	1504:1512	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	28	theme	interfering	1579:1589	arg1	compounds					1605:1613	potentially interfering electroactive compounds	1567:1613	potentially interfering electroactive compounds	1567:1613	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	29	from	range	1558:1562	arg1	sensor					1487:1492	the sensor	1483:1492	the sensor	1483:1492	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	29	from	range	1558:1562	arg1	sensitive					1504:1512	sensitive	1504:1512	sensitive	1504:1512	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	7	30	theme	MTZ	1265:1267	arg1	linear					1304:1309	linear	1304:1309	linear	1304:1309	The response of MTZ using the composite electrodes was linear over the range from 0.002 to 210.0 µM.
29960906	7	30	theme	MTZ	1265:1267	arg1	response					1253:1260	The response	1249:1260	The response of MTZ using the composite electrodes	1249:1298	The response of MTZ using the composite electrodes was linear over the range from 0.002 to 210.0 µM.
29960906	1	31	theme	inorganic	206:214	arg1	nanocomposites					228:241	inorganic and organic nanocomposites	206:241	inorganic and organic nanocomposites	206:241	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	0	32	theme	Novel	0:4	arg1	synthesis					22:30	Novel electrochemical synthesis	0:30	Novel electrochemical synthesis of copper oxide	0:46	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	5	33	theme	catalytic	941:949	arg1	activity					951:958	excellent catalytic activity	931:958	excellent catalytic activity	931:958	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	2	34	theme	CuO	402:404	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	5	35	theme	composite	890:898	arg1	electrode					909:917	composite modified electrode	890:917	the GR-β-CD/CuO NPs composite modified electrode	870:917	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	6	36	used	used	1162:1165	arg2	amperometry					1146:1156	amperometry	1146:1156	amperometry	1146:1156	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	7	37	from	210.0 µM	1340:1347	arg1	range					1320:1324	the range	1316:1324	the range from 0.002 to 210.0 µM	1316:1347	The response of MTZ using the composite electrodes was linear over the range from 0.002 to 210.0 µM.
29960906	3	38	theme	stable	593:598	arg1	composite					608:616	A stable GR-β-CD composite	591:616	A stable GR-β-CD composite	591:616	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	1	39	theme	organic	220:226	arg1	nanocomposites					228:241	inorganic and organic nanocomposites	206:241	inorganic and organic nanocomposites	206:241	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	5	40	theme	modified	900:907	arg1	electrode					909:917	composite modified electrode	890:917	the GR-β-CD/CuO NPs composite modified electrode	870:917	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	1	41	theme	organic	323:329	arg1	chemicals					331:339	organic chemicals	323:339	organic chemicals	323:339	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	0	42	theme	metronidazole	151:163	arg1	detection					122:130	trace-level detection	110:130	trace-level detection of antibiotic drug metronidazole	110:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	3	43	theme	GR-β-CD	600:606	arg1	composite					608:616	A stable GR-β-CD composite	591:616	A stable GR-β-CD composite	591:616	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	3	44	from	sonication	634:643	arg1	solution					667:674	β-CD aqueous solution	654:674	β-CD aqueous solution	654:674	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	1	45	theme	nanocomposites	228:241	arg1	synthesis					193:201	the synthesis	189:201	the synthesis of inorganic and organic nanocomposites	189:241	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	0	46	theme	oxide	42:46	arg1	synthesis					22:30	Novel electrochemical synthesis	0:30	Novel electrochemical synthesis of copper oxide	0:46	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	1	47	theme	chemicals	331:339	arg1	electroanalysis					304:318	electroanalysis	304:318	electroanalysis of organic chemicals	304:339	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	4	48	theme	high-resolution	742:756	arg1	microscopy					776:785	the high-resolution scanning electron microscopy	738:785	the high-resolution scanning electron microscopy	738:785	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	6	49	theme	NPs	1214:1216	arg1	electrodes					1237:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	5	50	theme	lower	964:968	arg1	potential					980:988	lower reduction potential	964:988	lower reduction potential	964:988	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	4	51	theme	GR-β-CD/CuO	689:699	arg1	composites					705:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	0	52	theme	copper	35:40	arg1	oxide					42:46	copper oxide	35:46	copper oxide	35:46	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	2	53	theme	modified	570:577	arg1	electrodes					579:588	GR-β-CD composite modified electrodes	552:588	GR-β-CD composite modified electrodes	552:588	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	8	54	theme	detection	1389:1397	arg1	limit					1380:1384	the lowest limit	1369:1384	the lowest limit of detection of 0.6 nM	1369:1407	This sensor showed the lowest limit of detection of 0.6 nM and was much lower than the previously reported MTZ sensors.
29960906	5	55	theme	CuO	1109:1111	arg1	NPs					1113:1115	CuO NPs	1109:1115	CuO NPs	1109:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	5	56	theme	excellent	931:939	arg1	activity					951:958	excellent catalytic activity	931:958	excellent catalytic activity	931:958	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	2	57	theme	graphene/β-cyclodextrin	421:443	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	0	58	theme	decorated	62:70	arg1	composite					96:104	decorated graphene-β-cyclodextrin composite	62:104	decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole	62:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	5	59	theme	reduction	970:978	arg1	potential					980:988	lower reduction potential	964:988	lower reduction potential	964:988	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	6	60	theme	MTZ	1192:1194	arg1	determination					1175:1187	the determination	1171:1187	the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes	1171:1246	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	5	61	theme	electrochemical	1002:1016	arg1	detection					1018:1026	the electrochemical detection	998:1026	the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs	998:1115	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	2	62	theme	electrochemical	481:495	arg1	methodology					497:507	a simple electrochemical methodology	472:507	a simple electrochemical methodology	472:507	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	9	63	theme	electroactive	1591:1603	arg1	compounds					1605:1613	potentially interfering electroactive compounds	1567:1613	potentially interfering electroactive compounds	1567:1613	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	7	64	theme	composite	1279:1287	arg1	electrodes					1289:1298	the composite electrodes	1275:1298	the composite electrodes	1275:1298	The response of MTZ using the composite electrodes was linear over the range from 0.002 to 210.0 µM.
29960906	8	65	theme	0.6 nM	1402:1407	arg1	detection					1389:1397	detection	1389:1397	detection of 0.6 nM	1389:1407	This sensor showed the lowest limit of detection of 0.6 nM and was much lower than the previously reported MTZ sensors.
29960906	5	66	dep	activity	951:958	arg1	an					928:929	an	928:929	an	928:929	Cyclic voltammetry results reveal that the GR-β-CD/CuO NPs composite modified electrode exhibits an excellent catalytic activity and lower reduction potential towards the electrochemical detection of metronidazole (MTZ) over other modified electrodes including GR, GR-β-CD, and CuO NPs.
29960906	9	67	dep	range	1558:1562	arg1	presence					1544:1551	presence	1544:1551	presence	1544:1551	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	9	67	dep	range	1558:1562	arg1	the					1540:1542	the	1540:1542	the	1540:1542	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	4	68	theme	electron	767:774	arg1	microscopy					776:785	the high-resolution scanning electron microscopy	738:785	the high-resolution scanning electron microscopy	738:785	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	3	69	theme	β-CD	654:657	arg1	solution					667:674	β-CD aqueous solution	654:674	β-CD aqueous solution	654:674	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	4	70	theme	X-ray	788:792	arg1	diffraction					794:804	X-ray diffraction	788:804	X-ray diffraction	788:804	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	4	71	theme	Raman	811:815	arg1	spectroscopy					817:828	Raman spectroscopy	811:828	Raman spectroscopy	811:828	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	1	72	theme	much	256:259	arg1	attention					261:269	much attention	256:269	much attention	256:269	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	9	73	theme	compounds	1605:1613	arg1	range					1558:1562	a range	1556:1562	a range of potentially interfering electroactive compounds	1556:1613	In addition, the sensor is highly sensitive, selective and durable in the presence of a range of potentially interfering electroactive compounds.
29960906	3	74	theme	aqueous	659:665	arg1	solution					667:674	β-CD aqueous solution	654:674	β-CD aqueous solution	654:674	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	2	75	theme	nanoparticle	388:399	arg1	composites					455:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites	375:464	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	6	76	theme	GR-β-CD/CuO	1202:1212	arg1	electrodes					1237:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	GR-β-CD/CuO NPs composite modified electrodes	1202:1246	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
29960906	7	77	dep	210.0 µM	1340:1347	arg1	to					1337:1338	to	1337:1338	to	1337:1338	The response of MTZ using the composite electrodes was linear over the range from 0.002 to 210.0 µM.
29960906	4	78	theme	As-prepared	677:687	arg1	composites					705:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites	677:714	As-prepared GR-β-CD/CuO NPs composites were characterized by the high-resolution scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
29960906	3	79	theme	GR	648:649	arg1	sonication					634:643	sonication	634:643	sonication of GR in β-CD aqueous solution	634:674	A stable GR-β-CD composite was prepared by sonication of GR in β-CD aqueous solution.
29960906	1	80	theme	past	175:178	arg1	decades					180:186	the past decades	171:186	the past decades	171:186	Over the past decades, the synthesis of inorganic and organic nanocomposites has received much attention in the range of fields including electroanalysis of organic chemicals.
29960906	8	81	theme	MTZ	1457:1459	arg1	sensors					1461:1467	the previously reported MTZ sensors	1433:1467	the previously reported MTZ sensors	1433:1467	This sensor showed the lowest limit of detection of 0.6 nM and was much lower than the previously reported MTZ sensors.
29960906	0	82	theme	graphene-β-cyclodextrin	72:94	arg1	composite					96:104	decorated graphene-β-cyclodextrin composite	62:104	decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole	62:163	Novel electrochemical synthesis of copper oxide nanoparticles decorated graphene-β-cyclodextrin composite for trace-level detection of antibiotic drug metronidazole.
29960906	2	83	theme	simple	474:479	arg1	methodology					497:507	a simple electrochemical methodology	472:507	a simple electrochemical methodology	472:507	In this regard, we have prepared copper oxide nanoparticle (CuO NPs) decorated graphene/β-cyclodextrin (GR-β-CD) composites using a simple electrochemical methodology, where the CuO NPs are electrodeposited on GR-β-CD composite modified electrodes.
29960906	6	84	theme	optimized	1124:1132	arg1	conditions					1134:1143	optimized conditions	1124:1143	optimized conditions	1124:1143	Under optimized conditions, amperometry was used for the determination of MTZ using GR-β-CD/CuO NPs composite modified electrodes.
31604508	0	0	theme	Composition	82:92	arg1	Variability					67:77	Variability	67:77	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	3	1	theme	PRP	500:502	arg1	compositions					484:495	different compositions	474:495	different compositions of PRP	474:502	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	3	1	theme	PRP	500:502	arg1	counts					523:528	absolute platelet counts	505:528	absolute platelet counts	505:528	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	3	1	theme	PRP	500:502	arg1	variables					576:584	many other physiological and demographic variables	535:584	many other physiological and demographic variables	535:584	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	4	2	contain	has	684:686	arg2	influence					702:710	a substantial influence	688:710	a substantial influence on outcome	688:721	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	4	2	contain	has	684:686	arg1	Variability					620:630	Variability	620:630	Variability of the composition of the ingredients of PRP	620:675	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	4	3	theme	substantial	690:700	arg1	influence					702:710	a substantial influence	688:710	a substantial influence on outcome	688:721	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	0	4	theme	Concentration	95:107	arg1	Variability					67:77	Variability	67:77	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	3	5	theme	many	535:538	arg1	variables					576:584	many other physiological and demographic variables	535:584	many other physiological and demographic variables	535:584	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	0	6	theme	Preparation	110:120	arg1	Variability					67:77	Variability	67:77	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	2	7	theme	hyaluronic	379:388	arg1	acid					390:393	hyaluronic acid	379:393	hyaluronic acid	379:393	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	1	8	theme	Platelet-rich	183:195	arg1	plasma					197:202	Platelet-rich plasma	183:202	Platelet-rich plasma (PRP)	183:208	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	1	8	theme	Platelet-rich	183:195	arg1	PRP					205:207	PRP	205:207	PRP	205:207	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	0	9	theme	Editorial	0:8	arg1	Commentary					10:19	Editorial Commentary	0:19	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	3	10	theme	demographic	564:574	arg1	variables					576:584	many other physiological and demographic variables	535:584	many other physiological and demographic variables	535:584	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	4	11	theme	PRP	673:675	arg1	ingredients					658:668	the ingredients	654:668	the ingredients of PRP	654:675	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	0	12	theme	Platelet-Rich	22:34	arg1	Plasma					36:41	Platelet-Rich Plasma	22:41	Platelet-Rich Plasma	22:41	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	3	13	theme	other	540:544	arg1	variables					576:584	many other physiological and demographic variables	535:584	many other physiological and demographic variables	535:584	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	2	14	theme	significant	406:416	arg1	relief					430:435	significant better pain relief	406:435	significant better pain relief	406:435	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	1	15	theme	clinical	282:289	arg1	studies					291:297	clinical studies	282:297	clinical studies for a variety of conditions	282:325	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	3	16	theme	physiological	546:558	arg1	variables					576:584	many other physiological and demographic variables	535:584	many other physiological and demographic variables	535:584	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	0	17	theme	Other	132:136	arg1	Factors					150:156	Many Other Yet-Unknown Factors	127:156	Many Other Yet-Unknown Factors	127:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	0	18	theme	Many	127:130	arg1	Factors					150:156	Many Other Yet-Unknown Factors	127:156	Many Other Yet-Unknown Factors	127:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	2	19	theme	pain	425:428	arg1	relief					430:435	significant better pain relief	406:435	significant better pain relief	406:435	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	0	20	theme	Factors	150:156	arg1	Variability					67:77	Variability	67:77	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	2	21	dep	significant	406:416	arg1	better					418:423	better	418:423	better	418:423	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	2	22	theme	knee	332:335	arg1	osteoarthritis					337:350	knee osteoarthritis	332:350	knee osteoarthritis	332:350	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	2	23	theme	functional	441:450	arg1	improvement					452:462	functional improvement	441:462	functional improvement	441:462	For knee osteoarthritis, it is more effective than hyaluronic acid, providing significant better pain relief and functional improvement.
31604508	0	24	theme	Yet-Unknown	138:148	arg1	Factors					150:156	Many Other Yet-Unknown Factors	127:156	Many Other Yet-Unknown Factors	127:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	0	25	dep	Plasma	36:41	arg1	Placebo					58:64	Placebo	58:64	Placebo	58:64	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	3	26	theme	different	474:482	arg1	compositions					484:495	different compositions	474:495	different compositions of PRP	474:502	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	1	27	used	used	224:227	arg2	PRP					205:207	PRP	205:207	PRP	205:207	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	1	27	used	used	224:227	arg2	plasma					197:202	Platelet-rich plasma	183:202	Platelet-rich plasma (PRP)	183:208	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	0	28	dep	Commentary	10:19	arg1	Profit-Rich					46:56	Profit-Rich	46:56	Profit-Rich	46:56	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	0	28	dep	Commentary	10:19	arg1	Variability					67:77	Variability	67:77	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors	0:156	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	0	28	dep	Commentary	10:19	arg1	Plasma					36:41	Platelet-Rich Plasma	22:41	Platelet-Rich Plasma	22:41	Editorial Commentary: Platelet-Rich Plasma or Profit-Rich Placebo: Variability of Composition, Concentration, Preparation, and Many Other Yet-Unknown Factors Determine Effectiveness.
31604508	4	29	theme	composition	639:649	arg1	Variability					620:630	Variability	620:630	Variability of the composition of the ingredients of PRP	620:675	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	3	30	theme	absolute	505:512	arg1	counts					523:528	absolute platelet counts	505:528	absolute platelet counts	505:528	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	4	31	theme	ingredients	658:668	arg1	composition					639:649	the composition	635:649	the composition of the ingredients of PRP	635:675	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
31604508	1	32	theme	conditions	316:325	arg1	conditions					316:325	conditions	316:325	conditions	316:325	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	1	32	theme	conditions	316:325	arg1	variety					305:311	a variety	303:311	a variety of conditions	303:325	Platelet-rich plasma (PRP) frequently is used in orthopaedics, and its application is supported by clinical studies for a variety of conditions.
31604508	3	33	theme	platelet	514:521	arg1	counts					523:528	absolute platelet counts	505:528	absolute platelet counts	505:528	However, different compositions of PRP, absolute platelet counts, and many other physiological and demographic variables will influence the effectiveness.
31604508	4	34	from	influence	702:710	arg1	outcome					715:721	outcome	715:721	outcome	715:721	Variability of the composition of the ingredients of PRP surely has a substantial influence on outcome.
30511062	11	0	theme	Transforming	1380:1391	arg1	Factor					1400:1405	Transforming Growth Factor β3	1380:1408	Transforming Growth Factor β3 (TGF-β3)	1380:1417	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	11	0	theme	Transforming	1380:1391	arg1	TGF-β3					1411:1416	TGF-β3	1411:1416	TGF-β3	1411:1416	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	11	1	theme	coatings	1286:1293	arg1	potential					1269:1277	The potential	1265:1277	The potential of the coatings as drug delivery system for therapeutic proteins	1265:1342	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	13	2	with	combination	1503:1513	arg1	deposition					1539:1548	the layer-by-layer deposition	1520:1548	the layer-by-layer deposition	1520:1548	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	12	3	theme	CS-TPP	1424:1429	arg1	nanogels					1431:1438	The CS-TPP nanogels	1420:1438	The CS-TPP nanogels	1420:1438	The CS-TPP nanogels were shown to encapsulate and release therapeutic proteins.
30511062	11	4	theme	Growth	1393:1398	arg1	Factor					1400:1405	Transforming Growth Factor β3	1380:1408	Transforming Growth Factor β3 (TGF-β3)	1380:1417	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	11	4	theme	Growth	1393:1398	arg1	TGF-β3					1411:1416	TGF-β3	1411:1416	TGF-β3	1411:1416	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	6	5	theme	ellipsometric	616:628	arg1	thickness					630:638	The ellipsometric thickness	612:638	The ellipsometric thickness	612:638	The ellipsometric thickness was demonstrated to depend significantly on the type of polyanion.
30511062	7	6	theme	XPS	707:709	arg1	analysis					711:718	XPS analysis	707:718	XPS analysis with depth profiling	707:739	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	3	7	theme	size	335:338	arg1	terms					317:321	terms	317:321	terms of particle size, zeta-potential and stability	317:368	The nanogels were characterized in terms of particle size, zeta-potential and stability.
30511062	4	8	theme	Nanogel	371:377	arg1	suspensions					379:389	Nanogel suspensions	371:389	Nanogel suspensions	371:389	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	9	9	theme	fiber	1060:1064	arg1	mats					1066:1069	PCL fiber mats	1056:1069	PCL fiber mats	1056:1069	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	13	10	from	transitions	1659:1669	arg1	applications					1636:1647	applications	1636:1647	applications at tissue transitions	1636:1669	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	4	11	theme	PCL	463:465	arg1	mats					473:476	PCL fiber mats	463:476	PCL fiber mats by LbL-deposition	463:494	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	11	12	theme	Factor	1400:1405	arg1	release					1369:1375	the release	1365:1375	the release of Transforming Growth Factor β3 (TGF-β3)	1365:1417	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	7	13	theme	depth	725:729	arg1	profiling					731:739	depth profiling	725:739	depth profiling	725:739	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	11	14	theme	therapeutic	1323:1333	arg1	proteins					1335:1342	therapeutic proteins	1323:1342	therapeutic proteins	1323:1342	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	13	15	theme	drug	1617:1620	arg1	gradients					1622:1630	drug gradients	1617:1630	drug gradients	1617:1630	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	3	16	theme	particle	326:333	arg1	size					335:338	particle size	326:338	particle size	326:338	The nanogels were characterized in terms of particle size, zeta-potential and stability.
30511062	5	17	theme	chondroitin	561:571	arg1	polyanions					542:551	polyanions	542:551	polyanions	542:551	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	5	17	theme	chondroitin	561:571	arg1	sulfate					573:579	chondroitin sulfate	561:579	chondroitin sulfate	561:579	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	3	18	theme	stability	360:368	arg1	terms					317:321	terms	317:321	terms of particle size, zeta-potential and stability	317:368	The nanogels were characterized in terms of particle size, zeta-potential and stability.
30511062	4	19	theme	fiber	467:471	arg1	mats					473:476	PCL fiber mats	463:476	PCL fiber mats by LbL-deposition	463:494	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	11	20	theme	drug	1298:1301	arg1	system					1312:1317	drug delivery system	1298:1317	drug delivery system for therapeutic proteins	1298:1342	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	7	21	with	films	814:818	arg1	polyanions					839:848	the different polyanions	825:848	the different polyanions	825:848	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	13	22	theme	PCL	1582:1584	arg1	implants					1596:1603	PCL fiber mat implants	1582:1603	PCL fiber mat implants having with drug gradients	1582:1630	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	6	23	theme	polyanion	696:704	arg1	type					688:691	the type	684:691	the type of polyanion	684:704	The ellipsometric thickness was demonstrated to depend significantly on the type of polyanion.
30511062	0	24	theme	Layer-by-layer	0:13	arg1	deposition					15:24	Layer-by-layer deposition	0:24	Layer-by-layer deposition of chitosan	0:36	Layer-by-layer deposition of chitosan nanoparticles as drug-release coatings for PCL nanofibers.
30511062	13	25	theme	implants	1596:1603	arg1	creation					1570:1577	the creation	1566:1577	the creation of PCL fiber mat implants having with drug gradients	1566:1630	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	1	26	with	gelation	134:141	arg1	TPP					183:185	TPP	183:185	TPP	183:185	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	1	26	with	gelation	134:141	arg1	tripolyphosphate					165:180	tripolyphosphate	165:180	tripolyphosphate (TPP)	165:186	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	13	27	theme	fiber	1586:1590	arg1	implants					1596:1603	PCL fiber mat implants	1582:1603	PCL fiber mat implants having with drug gradients	1582:1630	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	9	28	theme	chitosan-PCL-graft	1099:1116	arg1	polymer					1118:1124	a chitosan-PCL-graft polymer	1097:1124	a chitosan-PCL-graft polymer	1097:1124	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	3	29	theme	zeta-potential	341:354	arg1	terms					317:321	terms	317:321	terms of particle size, zeta-potential and stability	317:368	The nanogels were characterized in terms of particle size, zeta-potential and stability.
30511062	10	30	from	coatings	1173:1180	arg1	mats					1195:1198	the fiber mats	1185:1198	the fiber mats	1185:1198	The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles.
30511062	8	31	theme	ions	1009:1012	arg1	release					990:996	a complete exchange and release	966:996	release	990:996	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	8	31	theme	ions	1009:1012	arg1	exchange					977:984	a complete exchange and release	966:996	exchange	977:984	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	13	32	theme	mat	1592:1594	arg1	implants					1596:1603	PCL fiber mat implants	1582:1603	PCL fiber mat implants having with drug gradients	1582:1630	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	10	33	theme	fiber	1189:1193	arg1	mats					1195:1198	the fiber mats	1185:1198	the fiber mats	1185:1198	The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles.
30511062	4	34	theme	silicon	441:447	arg1	wafers					449:454	silicon wafers	441:454	silicon wafers	441:454	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	8	35	theme	polyanions	919:928	arg1	penetration					900:910	a strong penetration	891:910	a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions	891:1012	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	7	36	theme	different	829:837	arg1	polyanions					839:848	the different polyanions	825:848	the different polyanions	825:848	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	8	37	theme	strong	893:898	arg1	penetration					900:910	a strong penetration	891:910	a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions	891:1012	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	7	38	theme	films	814:818	arg1	composition					795:805	the chemical composition	782:805	the chemical composition of the films with the different polyanions	782:848	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	9	39	theme	PCL	1056:1058	arg1	mats					1066:1069	PCL fiber mats	1056:1069	PCL fiber mats	1056:1069	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	7	40	with	analysis	711:718	arg1	profiling					731:739	depth profiling	725:739	depth profiling	725:739	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	10	41	theme	CS-TPP	1243:1248	arg1	nanoparticles					1250:1262	fluorescently labelled CS-TPP nanoparticles	1220:1262	fluorescently labelled CS-TPP nanoparticles	1220:1262	The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles.
30511062	0	42	theme	chitosan	29:36	arg1	deposition					15:24	Layer-by-layer deposition	0:24	Layer-by-layer deposition of chitosan	0:36	Layer-by-layer deposition of chitosan nanoparticles as drug-release coatings for PCL nanofibers.
30511062	8	43	theme	CS-TPP	939:944	arg1	layer					946:950	the CS-TPP layer	935:950	the CS-TPP layer	935:950	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	9	44	mod	modified	1083:1090	arg3	polymer					1118:1124	a chitosan-PCL-graft polymer	1097:1124	a chitosan-PCL-graft polymer	1097:1124	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	9	44	mod	modified	1083:1090	arg1	mats					1066:1069	PCL fiber mats	1056:1069	PCL fiber mats	1056:1069	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	9	44	mod	modified	1083:1090	arg3	alginate					1130:1137	alginate	1130:1137	alginate	1130:1137	The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate.
30511062	4	45	from	multilayers	426:436	arg1	mats					473:476	PCL fiber mats	463:476	PCL fiber mats by LbL-deposition	463:494	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	4	45	from	multilayers	426:436	arg1	wafers					449:454	silicon wafers	441:454	silicon wafers	441:454	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	2	46	theme	nanogels	205:212	arg1	use					193:195	The use	189:195	The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL)	189:267	The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied.
30511062	1	47	theme	ionotropic	123:132	arg1	CS					156:157	CS	156:157	CS	156:157	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	1	47	theme	ionotropic	123:132	arg1	gelation					134:141	ionotropic gelation	123:141	ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP)	123:186	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	0	48	theme	drug-release	55:66	arg1	coatings					68:75	drug-release coatings	55:75	drug-release coatings for PCL nanofibers	55:94	Layer-by-layer deposition of chitosan nanoparticles as drug-release coatings for PCL nanofibers.
30511062	12	49	theme	therapeutic	1478:1488	arg1	proteins					1490:1497	therapeutic proteins	1478:1497	therapeutic proteins	1478:1497	The CS-TPP nanogels were shown to encapsulate and release therapeutic proteins.
30511062	2	50	theme	such	200:203	arg1	nanogels					205:212	such nanogels	200:212	such nanogels	200:212	The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied.
30511062	4	51	used	used	396:399	arg2	suspensions					379:389	Nanogel suspensions	371:389	Nanogel suspensions	371:389	Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition.
30511062	5	52	used	used	534:537	arg2	polyanions					542:551	polyanions	542:551	polyanions	542:551	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	5	52	used	used	534:537	arg2	polysaccharides					513:527	Three different polysaccharides	497:527	Three different polysaccharides	497:527	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	7	53	theme	chemical	786:793	arg1	composition					795:805	the chemical composition	782:805	the chemical composition of the films with the different polyanions	782:848	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	7	54	from	differences	767:777	arg1	composition					795:805	the chemical composition	782:805	the chemical composition of the films with the different polyanions	782:848	XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions.
30511062	8	55	theme	TPP	1005:1007	arg1	ions					1009:1012	the TPP ions	1001:1012	the TPP ions	1001:1012	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	13	56	theme	layer-by-layer	1524:1537	arg1	deposition					1539:1548	the layer-by-layer deposition	1520:1548	the layer-by-layer deposition	1520:1548	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	5	57	theme	hyaluronic	595:604	arg1	polyanions					542:551	polyanions	542:551	polyanions	542:551	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	5	57	theme	hyaluronic	595:604	arg1	acid					606:609	hyaluronic acid	595:609	hyaluronic acid	595:609	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	10	58	theme	labelled	1234:1241	arg1	nanoparticles					1250:1262	fluorescently labelled CS-TPP nanoparticles	1220:1262	fluorescently labelled CS-TPP nanoparticles	1220:1262	The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles.
30511062	13	59	theme	tissue	1652:1657	arg1	transitions					1659:1669	tissue transitions	1652:1669	tissue transitions	1652:1669	In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
30511062	2	60	theme	layer-by-layer	237:250	arg1	LbL					264:266	LbL	264:266	LbL	264:266	The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied.
30511062	2	60	theme	layer-by-layer	237:250	arg1	deposition					252:261	layer-by-layer deposition	237:261	layer-by-layer deposition (LbL)	237:267	The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied.
30511062	5	61	theme	different	503:511	arg1	polyanions					542:551	polyanions	542:551	polyanions	542:551	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	5	61	theme	different	503:511	arg1	polysaccharides					513:527	Three different polysaccharides	497:527	Three different polysaccharides	497:527	Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid.
30511062	1	62	theme	chitosan	146:153	arg1	CS					156:157	CS	156:157	CS	156:157	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	1	62	theme	chitosan	146:153	arg1	gelation					134:141	ionotropic gelation	123:141	ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP)	123:186	Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP).
30511062	0	63	theme	PCL	81:83	arg1	nanofibers					85:94	PCL nanofibers	81:94	PCL nanofibers	81:94	Layer-by-layer deposition of chitosan nanoparticles as drug-release coatings for PCL nanofibers.
30511062	11	64	theme	delivery	1303:1310	arg1	system					1312:1317	drug delivery system	1298:1317	drug delivery system for therapeutic proteins	1298:1342	The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3).
30511062	8	65	theme	complete	968:975	arg1	exchange					977:984	a complete exchange and release	966:996	exchange	977:984	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	8	66	theme	XPS	864:866	arg1	data					868:871	XPS data	864:871	XPS data	864:871	Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions.
30511062	10	67	theme	graded	1166:1171	arg1	coatings					1173:1180	graded coatings	1166:1180	graded coatings on the fiber mats	1166:1198	The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles.
29895145	0	0	theme	in	103:104	arg1	Synthesis					111:119	in Situ Synthesis	103:119	in Situ Synthesis of Poly(dopamine)	103:137	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	2	1	theme	signal	511:516	arg1	detection					518:526	electrophysiological signal detection	490:526	electrophysiological signal detection	490:526	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	2	2	theme	conductive	340:349	arg1	composite					365:373	a novel flexible and conductive biocompatible composite	319:373	a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability	319:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	1	3	theme	biosensing	245:254	arg1	materials					256:264	flexible biosensing materials	236:264	flexible biosensing materials	236:264	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	2	4	theme	electrophysiological	490:509	arg1	detection					518:526	electrophysiological signal detection	490:526	electrophysiological signal detection	490:526	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	4	5	theme	PDA	830:832	arg1	aggregation					815:825	the aggregation	811:825	the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	811:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	4	6	theme	wrapped	760:766	arg1	layer					768:772	a uniform and continuous wrapped layer	735:772	a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	735:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	4	7	theme	rapid	844:848	arg1	polymerization					850:863	rapid polymerization	844:863	rapid polymerization under the conventional alkaline condition	844:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	3	8	theme	in	564:565	arg1	self-polymerization					572:590	the in situ self-polymerization	560:590	the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition	560:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	7	9	theme	signal	1312:1317	arg1	testing					1319:1325	electrocardiogram signal testing	1294:1325	electrocardiogram signal testing	1294:1325	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	8	10	theme	conductive	1557:1566	arg1	biomaterials					1568:1579	flexible conductive biomaterials	1548:1579	flexible conductive biomaterials for wearable response devices	1548:1609	The BC/PDA-FCF provides a platform for the creation of flexible conductive biomaterials for wearable response devices.
29895145	3	11	theme	dopamine	595:602	arg1	self-polymerization					572:590	the in situ self-polymerization	560:590	the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition	560:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	7	12	theme	FCF	1372:1374	arg1	bioelectrode					1376:1387	the FCF bioelectrode	1368:1387	the FCF bioelectrode	1368:1387	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	8	13	theme	flexible	1548:1555	arg1	biomaterials					1568:1579	flexible conductive biomaterials	1548:1579	flexible conductive biomaterials for wearable response devices	1548:1609	The BC/PDA-FCF provides a platform for the creation of flexible conductive biomaterials for wearable response devices.
29895145	6	14	theme	flexible	1051:1058	arg1	FCF					1077:1079	FCF	1077:1079	FCF	1077:1079	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	14	theme	flexible	1051:1058	arg1	film					1071:1074	the flexible conductive film	1047:1074	the flexible conductive film (FCF)	1047:1080	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	5	15	from	suitable	967:974	arg1	addition					911:918	addition	911:918	addition	911:918	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	7	16	theme	testing	1319:1325	arg1	result					1284:1289	the result	1280:1289	the result of electrocardiogram signal testing	1280:1325	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	0	17	theme	Poly	124:127	arg1	Synthesis					111:119	in Situ Synthesis	103:119	in Situ Synthesis of Poly(dopamine)	103:137	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	7	18	theme	bioelectrode	1376:1387	arg1	property					1356:1363	the antibacterial property	1338:1363	the antibacterial property of the FCF bioelectrode	1338:1387	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	6	19	theme	electric	1184:1191	arg1	conductivity					1193:1204	a high electric conductivity	1177:1204	a high electric conductivity	1177:1204	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	4	20	theme	continuous	749:758	arg1	layer					768:772	a uniform and continuous wrapped layer	735:772	a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	735:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	6	21	with	S/cm	1237:1240	arg1	content					1258:1264	a high PDA content	1247:1264	a high PDA content	1247:1264	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	2	22	theme	ionic	395:399	arg1	ability					415:421	electronic and ionic bioconductive ability	380:421	electronic and ionic bioconductive ability	380:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	0	23	theme	Multifunctional	56:70	arg1	Bioelectrode					81:92	Multifunctional Flexible Bioelectrode	56:92	Multifunctional Flexible Bioelectrode	56:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	6	24	theme	PDA	1254:1256	arg1	content					1258:1264	a high PDA content	1247:1264	a high PDA content	1247:1264	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	7	25	theme	available	1472:1480	arg1	electrode					1482:1490	the commercially available electrode	1455:1490	the commercially available electrode	1455:1490	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	5	26	theme	special	946:952	arg1	structure					954:962	a special structure	944:962	a special structure	944:962	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	1	27	theme	rapid	172:176	arg1	development					178:188	the rapid development	168:188	the rapid development of the wearable detector and medical devices	168:233	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	0	28	theme	Electronic-Ionic	6:21	arg1	Composites					41:50	Novel Electronic-Ionic Hybrid Conductive Composites	0:50	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode	0:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	7	29	theme	excellent	1396:1404	arg1	comparable					1426:1435	comparable	1426:1435	comparable	1426:1435	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	7	29	theme	excellent	1396:1404	arg1	stability					1406:1414	an excellent stability	1393:1414	an excellent stability	1393:1414	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	0	30	dep	in	103:104	arg1	Situ					106:109	Situ	106:109	Situ	106:109	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	2	31	theme	electronic	380:389	arg1	ability					415:421	electronic and ionic bioconductive ability	380:421	electronic and ionic bioconductive ability	380:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	0	32	theme	Novel	0:4	arg1	Composites					41:50	Novel Electronic-Ionic Hybrid Conductive Composites	0:50	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode	0:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	1	33	theme	more	280:283	arg1	attention					294:302	more and more attention	280:302	more and more attention	280:302	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	6	34	theme	tensile	1103:1109	arg1	times					1132:1136	times	1132:1136	times	1132:1136	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	34	theme	tensile	1103:1109	arg1	strength					1111:1118	an extremely tensile strength	1090:1118	an extremely tensile strength	1090:1118	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	0	35	theme	Conductive	30:39	arg1	Composites					41:50	Novel Electronic-Ionic Hybrid Conductive Composites	0:50	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode	0:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	6	36	theme	high	1249:1252	arg1	content					1258:1264	a high PDA content	1247:1264	a high PDA content	1247:1264	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	37	theme	pure	1154:1157	arg1	BC					1159:1160	the pure BC	1150:1160	the pure BC	1150:1160	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	7	38	theme	electrocardiogram	1294:1310	arg1	testing					1319:1325	electrocardiogram signal testing	1294:1325	electrocardiogram signal testing	1294:1325	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	0	39	theme	Hybrid	23:28	arg1	Composites					41:50	Novel Electronic-Ionic Hybrid Conductive Composites	0:50	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode	0:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	1	40	theme	more	289:292	arg1	attention					294:302	more and more attention	280:302	more and more attention	280:302	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	4	41	theme	alkaline	888:895	arg1	condition					897:905	the conventional alkaline condition	871:905	the conventional alkaline condition	871:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	3	42	theme	neutral	659:665	arg1	pH					667:668	the neutral pH	655:668	the neutral pH condition	655:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	5	43	theme	fabricated	923:932	arg1	suitable					967:974	suitable	967:974	suitable	967:974	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	5	43	theme	fabricated	923:932	arg1	film					934:937	a fabricated film	921:937	a fabricated film with a special structure	921:962	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	1	44	theme	wearable	197:204	arg1	detector					206:213	the wearable detector	193:213	the wearable detector	193:213	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	6	45	dep	times	1132:1136	arg1	higher					1138:1143	higher	1138:1143	higher	1138:1143	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	3	46	theme	pH	667:668	arg1	condition					670:678	the neutral pH condition	655:678	the neutral pH condition	655:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	8	47	theme	wearable	1585:1592	arg1	devices					1603:1609	wearable response devices	1585:1609	wearable response devices	1585:1609	The BC/PDA-FCF provides a platform for the creation of flexible conductive biomaterials for wearable response devices.
29895145	1	48	theme	detector	206:213	arg1	development					178:188	the rapid development	168:188	the rapid development of the wearable detector and medical devices	168:233	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	6	49	theme	high	1179:1182	arg1	conductivity					1193:1204	a high electric conductivity	1177:1204	a high electric conductivity	1177:1204	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	50	theme	S/cm	1237:1240	arg1	value					1223:1227	a value	1221:1227	a value of 10-3 S/cm with a high PDA content	1221:1264	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	3	51	theme	bacterial	624:632	arg1	BC					645:646	BC	645:646	BC	645:646	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	3	51	theme	bacterial	624:632	arg1	cellulose					634:642	bacterial cellulose	624:642	bacterial cellulose (BC)	624:647	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	2	52	theme	bioconductive	401:413	arg1	ability					415:421	electronic and ionic bioconductive ability	380:421	electronic and ionic bioconductive ability	380:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	0	53	theme	Flexible	72:79	arg1	Bioelectrode					81:92	Multifunctional Flexible Bioelectrode	56:92	Multifunctional Flexible Bioelectrode	56:92	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	6	54	theme	10-3	1232:1235	arg1	S/cm					1237:1240	10-3 S/cm	1232:1240	10-3 S/cm with a high PDA content	1232:1264	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	2	55	theme	flexible	327:334	arg1	composite					365:373	a novel flexible and conductive biocompatible composite	319:373	a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability	319:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	2	56	theme	flexible	459:466	arg1	bioelectrode					468:479	a new flexible bioelectrode	453:479	a new flexible bioelectrode used for electrophysiological signal detection	453:526	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	0	57	theme	Bacterial	142:150	arg1	Cellulose					152:160	Bacterial Cellulose	142:160	Bacterial Cellulose	142:160	Novel Electronic-Ionic Hybrid Conductive Composites for Multifunctional Flexible Bioelectrode Based on in Situ Synthesis of Poly(dopamine) on Bacterial Cellulose.
29895145	5	58	theme	electrons	1002:1010	arg1	transportation					984:997	the transportation	980:997	the transportation of electrons and ions existing in it	980:1034	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	2	59	theme	novel	321:325	arg1	composite					365:373	a novel flexible and conductive biocompatible composite	319:373	a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability	319:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	3	60	from	self-polymerization	572:590	arg1	nanofiber					611:619	the nanofiber	607:619	the nanofiber of bacterial cellulose (BC) under the neutral pH condition	607:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	7	61	theme	antibacterial	1342:1354	arg1	property					1356:1363	the antibacterial property	1338:1363	the antibacterial property of the FCF bioelectrode	1338:1387	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	4	62	theme	conventional	875:886	arg1	condition					897:905	the conventional alkaline condition	871:905	the conventional alkaline condition	871:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	5	63	theme	ions	1016:1019	arg1	transportation					984:997	the transportation	980:997	the transportation of electrons and ions existing in it	980:1034	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	4	64	theme	BC	781:782	arg1	nanofiber					784:792	the BC nanofiber	777:792	the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	777:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	3	65	dep	in	564:565	arg1	situ					567:570	situ	567:570	situ	567:570	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	7	66	contain	has	1389:1391	arg2	stability					1406:1414	an excellent stability	1393:1414	an excellent stability	1393:1414	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	7	66	contain	has	1389:1391	arg2	comparable					1426:1435	comparable	1426:1435	comparable	1426:1435	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	7	66	contain	has	1389:1391	arg1	property					1356:1363	the antibacterial property	1338:1363	the antibacterial property of the FCF bioelectrode	1338:1387	Furthermore, the result of electrocardiogram signal testing shows that the antibacterial property of the FCF bioelectrode has an excellent stability, which is comparable to or better than the commercially available electrode.
29895145	1	67	theme	medical	219:225	arg1	devices					227:233	medical devices	219:233	medical devices	219:233	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	2	68	with	composite	365:373	arg1	ability					415:421	electronic and ionic bioconductive ability	380:421	electronic and ionic bioconductive ability	380:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	4	69	theme	uniform	737:743	arg1	layer					768:772	a uniform and continuous wrapped layer	735:772	a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	735:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	2	70	theme	biocompatible	351:363	arg1	composite					365:373	a novel flexible and conductive biocompatible composite	319:373	a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability	319:421	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	5	71	from	addition	911:918	arg1	suitable					967:974	suitable	967:974	suitable	967:974	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	5	71	from	addition	911:918	arg1	film					934:937	a fabricated film	921:937	a fabricated film with a special structure	921:962	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	1	72	theme	devices	227:233	arg1	development					178:188	the rapid development	168:188	the rapid development of the wearable detector and medical devices	168:233	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
29895145	6	73	theme	conductive	1060:1069	arg1	FCF					1077:1079	FCF	1077:1079	FCF	1077:1079	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	73	theme	conductive	1060:1069	arg1	film					1071:1074	the flexible conductive film	1047:1074	the flexible conductive film (FCF)	1047:1080	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	3	74	theme	cellulose	634:642	arg1	nanofiber					611:619	the nanofiber	607:619	the nanofiber of bacterial cellulose (BC) under the neutral pH condition	607:678	This composite was prepared by the in situ self-polymerization of dopamine on the nanofiber of bacterial cellulose (BC) under the neutral pH condition.
29895145	8	75	theme	response	1594:1601	arg1	devices					1603:1609	wearable response devices	1585:1609	wearable response devices	1585:1609	The BC/PDA-FCF provides a platform for the creation of flexible conductive biomaterials for wearable response devices.
29895145	8	76	theme	biomaterials	1568:1579	arg1	creation					1536:1543	the creation	1532:1543	the creation of flexible conductive biomaterials for wearable response devices	1532:1609	The BC/PDA-FCF provides a platform for the creation of flexible conductive biomaterials for wearable response devices.
29895145	5	77	with	film	934:937	arg1	structure					954:962	a special structure	944:962	a special structure	944:962	In addition, a fabricated film with a special structure is suitable for the transportation of electrons and ions existing in it.
29895145	6	78	theme	2	1130:1130	arg1	times					1132:1136	times	1132:1136	times	1132:1136	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	6	78	theme	2	1130:1130	arg1	strength					1111:1118	an extremely tensile strength	1090:1118	an extremely tensile strength	1090:1118	Moreover, the flexible conductive film (FCF) reveals an extremely tensile strength, which is 2 times higher than the pure BC in addition to a high electric conductivity, which reaches a value of 10-3 S/cm with a high PDA content.
29895145	4	79	from	layer	768:772	arg1	nanofiber					784:792	the BC nanofiber	777:792	the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition	777:905	By using this method, poly(dopamine) (PDA) could form a uniform and continuous wrapped layer on the BC nanofiber that can prevent the aggregation of PDA caused by rapid polymerization under the conventional alkaline condition.
29895145	2	80	theme	new	455:457	arg1	bioelectrode					468:479	a new flexible bioelectrode	453:479	a new flexible bioelectrode used for electrophysiological signal detection	453:526	In this work, a novel flexible and conductive biocompatible composite with electronic and ionic bioconductive ability was demonstrated to fabricate a new flexible bioelectrode used for electrophysiological signal detection.
29895145	1	81	theme	flexible	236:243	arg1	materials					256:264	flexible biosensing materials	236:264	flexible biosensing materials	236:264	With the rapid development of the wearable detector and medical devices, flexible biosensing materials have received more and more attention.
31330875	1	0	theme	cell	173:176	arg1	growth					178:183	cell growth	173:183	cell growth	173:183	Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization.
31330875	4	1	theme	resulting	539:547	arg1	gels					549:552	The resulting gels	535:552	The resulting gels	535:552	The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility.
31330875	5	2	contain	had	720:722	arg2	sizes					729:733	pore sizes	724:733	pore sizes of up to several hundred micrometers	724:770	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	5	2	contain	had	720:722	arg1	scaffolds					703:711	the scaffolds	699:711	the scaffolds	699:711	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	6	3	theme	Mechanical	773:782	arg1	testing					784:790	Mechanical testing	773:790	Mechanical testing	773:790	Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites.
31330875	2	4	theme	3D	328:329	arg1	matrix					331:336	a 3D matrix	326:336	a 3D matrix	326:336	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	2	4	theme	3D	328:329	arg1	that					304:307	that	304:307	that	304:307	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	9	5	theme	kinetic	1104:1110	arg1	models					1112:1117	Different release kinetic models	1086:1117	Different release kinetic models	1086:1117	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	1	6	theme	large	86:90	arg1	defects					97:103	large bone defects	86:103	large bone defects	86:103	Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization.
31330875	6	7	theme	lyophilized	837:847	arg1	composites					849:858	lyophilized composites	837:858	lyophilized composites	837:858	Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites.
31330875	5	8	dep	hundred	752:758	arg1	to					741:742	to	741:742	to	741:742	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	1	9	theme	bone	92:95	arg1	defects					97:103	large bone defects	86:103	large bone defects	86:103	Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization.
31330875	0	10	theme	Agarose/Hydroxyapatite	14:35	arg1	Composite					37:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite	0:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite	0:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release.
31330875	8	11	theme	model	1032:1036	arg1	compounds					1043:1051	model drug compounds	1032:1051	model drug compounds for guided hMSC differentiation	1032:1083	Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation.
31330875	1	12	theme	defects	97:103	arg1	Healing					75:81	Healing	75:81	Healing of large bone defects	75:103	Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization.
31330875	0	13	theme	Non-Cytotoxic	0:12	arg1	Composite					37:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite	0:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite	0:45	Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release.
31330875	7	14	theme	mesenchymal	886:896	arg1	cells					903:907	Lw35human mesenchymal stem cells	876:907	Lw35human mesenchymal stem cells (hMSCs)	876:915	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	14	theme	mesenchymal	886:896	arg1	hMSCs					910:914	hMSCs	910:914	hMSCs	910:914	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	15	from	testing	865:871	arg1	cells					903:907	Lw35human mesenchymal stem cells	876:907	Lw35human mesenchymal stem cells (hMSCs)	876:915	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	15	from	testing	865:871	arg1	cells					940:944	osteosarcoma MG-63 cells	921:944	osteosarcoma MG-63 cells	921:944	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	15	from	testing	865:871	arg1	hMSCs					910:914	hMSCs	910:914	hMSCs	910:914	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	3	16	theme	agarose	415:421	arg1	gels					423:426	the agarose gels	411:426	the agarose gels	411:426	Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying.
31330875	5	17	theme	HA	678:679	arg1	phase					658:662	A pure phase	651:662	A pure phase of carbonated HA	651:679	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	7	18	theme	stem	898:901	arg1	cells					903:907	Lw35human mesenchymal stem cells	876:907	Lw35human mesenchymal stem cells (hMSCs)	876:915	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	18	theme	stem	898:901	arg1	hMSCs					910:914	hMSCs	910:914	hMSCs	910:914	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	4	19	theme	mechanical	606:615	arg1	properties					617:626	mechanical properties	606:626	mechanical properties	606:626	The resulting gels were characterized regarding composition, porosity, mechanical properties, and biocompatibility.
31330875	8	20	theme	drug	1038:1041	arg1	compounds					1043:1051	model drug compounds	1032:1051	model drug compounds for guided hMSC differentiation	1032:1083	Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation.
31330875	6	21	theme	MPa	829:831	arg1	moduli					809:814	elastic moduli	801:814	elastic moduli of up to 2.8 MPa for lyophilized composites	801:858	Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites.
31330875	0	22	theme	Drug	61:64	arg1	Release					66:72	Drug Release	61:72	Drug Release	61:72	Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release.
31330875	8	23	theme	guided	1057:1062	arg1	differentiation					1069:1083	guided hMSC differentiation	1057:1083	guided hMSC differentiation	1057:1083	Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation.
31330875	9	24	theme	Different	1086:1094	arg1	models					1112:1117	Different release kinetic models	1086:1117	Different release kinetic models	1086:1117	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	9	25	theme	sustained	1201:1209	arg1	behavior					1219:1226	a sustained release behavior	1199:1226	a sustained release behavior	1199:1226	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	9	26	theme	release	1096:1102	arg1	models					1112:1117	Different release kinetic models	1086:1117	Different release kinetic models	1086:1117	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	5	27	theme	pure	653:656	arg1	phase					658:662	A pure phase	651:662	A pure phase of carbonated HA	651:679	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	9	28	theme	release	1211:1217	arg1	behavior					1219:1226	a sustained release behavior	1199:1226	a sustained release behavior	1199:1226	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	7	29	theme	scaffolds	981:989	arg1	biocompatibility					957:972	the biocompatibility	953:972	the biocompatibility of the scaffolds	953:989	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	3	30	theme	simple	459:464	arg1	procedure					466:474	a simple procedure	457:474	a simple procedure consisting of precipitation, cooling, washing, and drying	457:532	Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying.
31330875	2	31	theme	release	353:359	arg1	that					304:307	that	304:307	that	304:307	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	2	31	theme	release	353:359	arg1	system					361:366	a release system	351:366	a release system	351:366	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	8	32	theme	hMSC	1064:1067	arg1	differentiation					1069:1083	guided hMSC differentiation	1057:1083	guided hMSC differentiation	1057:1083	Furthermore, scaffolds were loaded with model drug compounds for guided hMSC differentiation.
31330875	2	33	theme	present	231:237	arg1	work					239:242	the present work	227:242	the present work	227:242	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	6	34	dep	2.8	825:827	arg1	to					822:823	to	822:823	to	822:823	Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites.
31330875	7	35	theme	osteosarcoma	921:932	arg1	cells					940:944	osteosarcoma MG-63 cells	921:944	osteosarcoma MG-63 cells	921:944	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	36	theme	Lw35human	876:884	arg1	cells					903:907	Lw35human mesenchymal stem cells	876:907	Lw35human mesenchymal stem cells (hMSCs)	876:915	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	7	36	theme	Lw35human	876:884	arg1	hMSCs					910:914	hMSCs	910:914	hMSCs	910:914	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	5	37	theme	carbonated	667:676	arg1	HA					678:679	carbonated HA	667:679	carbonated HA	667:679	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	6	38	theme	elastic	801:807	arg1	moduli					809:814	elastic moduli	801:814	elastic moduli of up to 2.8 MPa for lyophilized composites	801:858	Mechanical testing revealed elastic moduli of up to 2.8 MPa for lyophilized composites.
31330875	9	39	theme	adenosine	1138:1146	arg1	ATP					1165:1167	ATP	1165:1167	ATP	1165:1167	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	9	39	theme	adenosine	1138:1146	arg1	5'-triphosphate					1148:1162	adenosine 5'-triphosphate	1138:1162	adenosine 5'-triphosphate (ATP)	1138:1168	Different release kinetic models were evaluated for adenosine 5'-triphosphate (ATP) and suramin, and data showed a sustained release behavior over four days.
31330875	1	40	theme	structural	149:158	arg1	guidance					160:167	structural guidance	149:167	structural guidance for cell growth, differentiation, and vascularization	149:221	Healing of large bone defects requires implants or scaffolds that provide structural guidance for cell growth, differentiation, and vascularization.
31330875	7	41	theme	MTT	861:863	arg1	testing					865:871	MTT testing	861:871	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells	861:944	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	2	42	theme	composite	271:279	arg1	scaffold					281:288	an agarose-hydroxyapatite composite scaffold	245:288	an agarose-hydroxyapatite composite scaffold	245:288	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	3	43	theme	various	439:445	arg1	ratios					447:452	various ratios	439:452	various ratios	439:452	Hydroxyapatite (HA) was incorporated into the agarose gels in situ in various ratios by a simple procedure consisting of precipitation, cooling, washing, and drying.
31330875	5	44	theme	pore	724:727	arg1	sizes					729:733	pore sizes	724:733	pore sizes of up to several hundred micrometers	724:770	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
31330875	2	45	theme	agarose-hydroxyapatite	248:269	arg1	scaffold					281:288	an agarose-hydroxyapatite composite scaffold	245:288	an agarose-hydroxyapatite composite scaffold	245:288	In the present work, an agarose-hydroxyapatite composite scaffold was developed that acts not only as a 3D matrix, but also as a release system.
31330875	7	46	theme	MG-63	934:938	arg1	cells					940:944	osteosarcoma MG-63 cells	921:944	osteosarcoma MG-63 cells	921:944	MTT testing on Lw35human mesenchymal stem cells (hMSCs) and osteosarcoma MG-63 cells proved the biocompatibility of the scaffolds.
31330875	5	47	theme	micrometers	760:770	arg1	sizes					729:733	pore sizes	724:733	pore sizes of up to several hundred micrometers	724:770	A pure phase of carbonated HA was identified in the scaffolds, which had pore sizes of up to several hundred micrometers.
30703424	5	0	theme	rhamnogalacturonan	1124:1141	arg1	domains					1154:1160	rhamnogalacturonan II (RG-II) domains	1124:1160	rhamnogalacturonan II (RG-II) domains	1124:1160	WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3.
30703424	1	1	theme	flower	159:164	arg1	WGFPA					172:176	WGFPA	172:176	WGFPA	172:176	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	1	theme	flower	159:164	arg1	buds					166:169	Panax ginseng flower buds	145:169	Panax ginseng flower buds (WGFPA)	145:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	5	2	theme	different	1104:1112	arg1	ratios					1114:1119	different ratios	1104:1119	different ratios of rhamnogalacturonan II (RG-II) domains	1104:1160	WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3.
30703424	0	3	theme	binding	60:66	arg1	activities					68:77	their binding activities	54:77	their binding activities to galectin-3	54:91	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	4	4	dep	values	960:965	arg1	0.24 μM					987:993	0.24 μM	987:993	0.24 μM	987:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	4	dep	values	960:965	arg1	values					960:965	apparent KD values	948:965	apparent KD values 4.9 μM, 0.71 μM and 0.24 μM	948:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	4	dep	values	960:965	arg1	0.71 μM					975:981	0.71 μM	975:981	0.71 μM	975:981	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	4	dep	values	960:965	arg1	4.9 μM					967:972	4.9 μM	967:972	4.9 μM	967:972	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	5	theme	rhamnogalacturonan	722:739	arg1	pectin					755:760	rhamnogalacturonan I (RG-I) type pectin	722:760	rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3	722:941	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	1	6	theme	ion-exchange	323:334	arg1	combination					308:318	a combination	306:318	a combination of ion-exchange and size exclusion chromatographies	306:370	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	6	7	theme	structure-activity	1297:1314	arg1	relationship					1316:1327	the structure-activity relationship	1293:1327	the structure-activity relationship of ginseng flower buds pectin	1293:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	4	8	theme	abundant	767:774	arg1	β-D-1,4-galactan					817:832	β-D-1,4-galactan	817:832	β-D-1,4-galactan	817:832	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	8	theme	abundant	767:774	arg1	arabinogalactan					835:849	arabinogalactan I	835:851	arabinogalactan I (AG-I)	835:858	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	8	theme	abundant	767:774	arg1	arabinogalactan					864:878	arabinogalactan II	864:881	arabinogalactan II (AG-II)	864:889	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	8	theme	abundant	767:774	arg1	chains					781:786	abundant side chains	767:786	abundant side chains	767:786	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	8	theme	abundant	767:774	arg1	α-L-1,5-arabinan					799:814	α-L-1,5-arabinan	799:814	α-L-1,5-arabinan	799:814	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	0	9	from	buds	45:48	arg1	Analysis					0:7	Analysis	0:7	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.	0:92	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	4	10	theme	I	741:741	arg1	pectin					755:760	rhamnogalacturonan I (RG-I) type pectin	722:760	rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3	722:941	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	2	11	theme	structural	516:525	arg1	features					527:534	their structural features	510:534	their structural features	510:534	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	1	12	theme	size	340:343	arg1	exclusion					345:353	size exclusion	340:353	size exclusion chromatographies	340:370	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	4	13	with	pectin	755:760	arg1	arabinogalactan					835:849	arabinogalactan I	835:851	arabinogalactan I (AG-I)	835:858	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	arabinogalactan					864:878	arabinogalactan II	864:881	arabinogalactan II (AG-II)	864:889	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	chains					781:786	abundant side chains	767:786	abundant side chains	767:786	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	0.24 μM					987:993	0.24 μM	987:993	0.24 μM	987:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	α-L-1,5-arabinan					799:814	α-L-1,5-arabinan	799:814	α-L-1,5-arabinan	799:814	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	values					960:965	apparent KD values	948:965	apparent KD values 4.9 μM, 0.71 μM and 0.24 μM	948:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	4.9 μM					967:972	4.9 μM	967:972	4.9 μM	967:972	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	0.71 μM					975:981	0.71 μM	975:981	0.71 μM	975:981	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	13	with	pectin	755:760	arg1	β-D-1,4-galactan					817:832	β-D-1,4-galactan	817:832	β-D-1,4-galactan	817:832	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	0	14	theme	flower	38:43	arg1	buds					45:48	Panax ginseng flower buds	24:48	Panax ginseng flower buds	24:48	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	1	15	theme	exclusion	345:353	arg1	chromatographies					355:370	size exclusion chromatographies	340:370	size exclusion chromatographies	340:370	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	16	theme	chromatographies	355:370	arg1	combination					308:318	a combination	306:318	a combination of ion-exchange and size exclusion chromatographies	306:370	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	6	17	theme	further	1268:1274	arg1	investigation					1276:1288	further investigation	1268:1288	further investigation	1268:1288	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	6	18	theme	buds	1347:1350	arg1	pectin					1352:1357	ginseng flower buds pectin	1332:1357	ginseng flower buds pectin	1332:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	2	19	theme	Monosaccharide	373:386	arg1	composition					388:398	Monosaccharide composition	373:398	Monosaccharide composition	373:398	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	2	20	theme	resonance	447:455	arg1	analysis					471:478	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	2	21	theme	magnetic	438:445	arg1	resonance					447:455	13C nuclear magnetic resonance	426:455	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	2	21	theme	magnetic	438:445	arg1	NMR					458:460	NMR	458:460	NMR	458:460	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	4	22	theme	side	776:779	arg1	β-D-1,4-galactan					817:832	β-D-1,4-galactan	817:832	β-D-1,4-galactan	817:832	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	22	theme	side	776:779	arg1	arabinogalactan					835:849	arabinogalactan I	835:851	arabinogalactan I (AG-I)	835:858	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	22	theme	side	776:779	arg1	arabinogalactan					864:878	arabinogalactan II	864:881	arabinogalactan II (AG-II)	864:889	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	22	theme	side	776:779	arg1	chains					781:786	abundant side chains	767:786	abundant side chains	767:786	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	22	theme	side	776:779	arg1	α-L-1,5-arabinan					799:814	α-L-1,5-arabinan	799:814	α-L-1,5-arabinan	799:814	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	2	23	theme	13C	426:428	arg1	resonance					447:455	13C nuclear magnetic resonance	426:455	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	2	23	theme	13C	426:428	arg1	NMR					458:460	NMR	458:460	NMR	458:460	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	0	24	theme	pectin	12:17	arg1	Analysis					0:7	Analysis	0:7	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.	0:92	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	0	25	from	activities	68:77	arg1	Analysis					0:7	Analysis	0:7	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.	0:92	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	1	26	theme	Water-soluble	94:106	arg1	polysaccharides					115:129	Water-soluble pectic polysaccharides	94:129	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA)	94:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	0	27	theme	ginseng	30:36	arg1	buds					45:48	Panax ginseng flower buds	24:48	Panax ginseng flower buds	24:48	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	4	28	theme	KD	957:958	arg1	0.24 μM					987:993	0.24 μM	987:993	0.24 μM	987:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	28	theme	KD	957:958	arg1	values					960:965	apparent KD values	948:965	apparent KD values 4.9 μM, 0.71 μM and 0.24 μM	948:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	28	theme	KD	957:958	arg1	0.71 μM					975:981	0.71 μM	975:981	0.71 μM	975:981	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	28	theme	KD	957:958	arg1	4.9 μM					967:972	4.9 μM	967:972	4.9 μM	967:972	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	29	theme	apparent	948:955	arg1	0.24 μM					987:993	0.24 μM	987:993	0.24 μM	987:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	29	theme	apparent	948:955	arg1	values					960:965	apparent KD values	948:965	apparent KD values 4.9 μM, 0.71 μM and 0.24 μM	948:993	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	29	theme	apparent	948:955	arg1	0.71 μM					975:981	0.71 μM	975:981	0.71 μM	975:981	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	29	theme	apparent	948:955	arg1	4.9 μM					967:972	4.9 μM	967:972	4.9 μM	967:972	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	0	30	theme	Panax	24:28	arg1	buds					45:48	Panax ginseng flower buds	24:48	Panax ginseng flower buds	24:48	Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
30703424	5	31	theme	domains	1154:1160	arg1	ratios					1114:1119	different ratios	1104:1119	different ratios of rhamnogalacturonan II (RG-II) domains	1104:1160	WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3.
30703424	2	32	theme	spectra	463:469	arg1	analysis					471:478	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	6	33	theme	structural	1241:1250	arg1	information					1252:1262	useful structural information	1234:1262	useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin	1234:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	2	34	theme	enzymatic	401:409	arg1	hydrolysis					411:420	enzymatic hydrolysis	401:420	enzymatic hydrolysis	401:420	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	4	35	theme	binding	910:916	arg1	activities					918:927	strong binding activities	903:927	strong binding activities	903:927	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-3b					293:300	WGFPA-3b	293:300	WGFPA-3b	293:300	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	fractions					229:237	six homogeneous fractions	213:237	six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b)	213:301	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-1a					240:247	WGFPA-1a	240:247	WGFPA-1a	240:247	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-1b					270:277	WGFPA-1b	270:277	WGFPA-1b	270:277	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-2b					280:287	WGFPA-2b	280:287	WGFPA-2b	280:287	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-2a					250:257	WGFPA-2a	250:257	WGFPA-2a	250:257	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	36	dep	fractions	229:237	arg1	WGFPA-3a					260:267	WGFPA-3a	260:267	WGFPA-3a	260:267	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	6	37	theme	useful	1234:1239	arg1	information					1252:1262	useful structural information	1234:1262	useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin	1234:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	6	38	theme	flower	1340:1345	arg1	pectin					1352:1357	ginseng flower buds pectin	1332:1357	ginseng flower buds pectin	1332:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	1	39	theme	pectic	108:113	arg1	polysaccharides					115:129	Water-soluble pectic polysaccharides	94:129	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA)	94:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	40	attach	isolated	131:138	arg1	WGFPA					172:176	WGFPA	172:176	WGFPA	172:176	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	40	attach	isolated	131:138	arg2	polysaccharides					115:129	Water-soluble pectic polysaccharides	94:129	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA)	94:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	40	attach	isolated	131:138	arg1	buds					166:169	Panax ginseng flower buds	145:169	Panax ginseng flower buds (WGFPA)	145:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	4	41	theme	strong	903:908	arg1	activities					918:927	strong binding activities	903:927	strong binding activities	903:927	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	5	42	theme	homogalacturonan	1047:1062	arg1	pectin					1074:1079	homogalacturonan (HG) type pectin	1047:1079	homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains	1047:1160	WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3.
30703424	3	43	theme	interferometry	639:652	arg1	assay					654:658	biolayer interferometry assay	630:658	biolayer interferometry assay	630:658	Furthermore, the interactions between these polysaccharides and galectin-3 were evaluated by biolayer interferometry assay.
30703424	4	44	theme	RG-I	744:747	arg1	pectin					755:760	rhamnogalacturonan I (RG-I) type pectin	722:760	rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3	722:941	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	4	45	theme	type	750:753	arg1	pectin					755:760	rhamnogalacturonan I (RG-I) type pectin	722:760	rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3	722:941	The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-3b					293:300	WGFPA-3b	293:300	WGFPA-3b	293:300	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	fractions					229:237	six homogeneous fractions	213:237	six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b)	213:301	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-1a					240:247	WGFPA-1a	240:247	WGFPA-1a	240:247	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-1b					270:277	WGFPA-1b	270:277	WGFPA-1b	270:277	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-2b					280:287	WGFPA-2b	280:287	WGFPA-2b	280:287	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-2a					250:257	WGFPA-2a	250:257	WGFPA-2a	250:257	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	46	theme	homogeneous	217:227	arg1	WGFPA-3a					260:267	WGFPA-3a	260:267	WGFPA-3a	260:267	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	6	47	theme	ginseng	1332:1338	arg1	pectin					1352:1357	ginseng flower buds pectin	1332:1357	ginseng flower buds pectin	1332:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	6	48	from	information	1252:1262	arg1	relationship					1316:1327	the structure-activity relationship	1293:1327	the structure-activity relationship of ginseng flower buds pectin	1293:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	5	49	theme	type	1069:1072	arg1	pectin					1074:1079	homogalacturonan (HG) type pectin	1047:1079	homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains	1047:1160	WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3.
30703424	3	50	theme	biolayer	630:637	arg1	assay					654:658	biolayer interferometry assay	630:658	biolayer interferometry assay	630:658	Furthermore, the interactions between these polysaccharides and galectin-3 were evaluated by biolayer interferometry assay.
30703424	1	51	theme	Panax	145:149	arg1	WGFPA					172:176	WGFPA	172:176	WGFPA	172:176	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	51	theme	Panax	145:149	arg1	buds					166:169	Panax ginseng flower buds	145:169	Panax ginseng flower buds (WGFPA)	145:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	6	52	theme	pectin	1352:1357	arg1	relationship					1316:1327	the structure-activity relationship	1293:1327	the structure-activity relationship of ginseng flower buds pectin	1293:1357	This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
30703424	2	53	theme	nuclear	430:436	arg1	resonance					447:455	13C nuclear magnetic resonance	426:455	13C nuclear magnetic resonance (NMR) spectra analysis	426:478	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	2	53	theme	nuclear	430:436	arg1	NMR					458:460	NMR	458:460	NMR	458:460	Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features.
30703424	1	54	theme	ginseng	151:157	arg1	WGFPA					172:176	WGFPA	172:176	WGFPA	172:176	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30703424	1	54	theme	ginseng	151:157	arg1	buds					166:169	Panax ginseng flower buds	145:169	Panax ginseng flower buds (WGFPA)	145:177	Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies.
30842558	0	0	theme	Cellulose	68:76	arg1	Nanofibrils					78:88	Cellulose Nanofibrils	68:88	Cellulose Nanofibrils	68:88	Fully Bio-Based Hybrid Composites Made of Wood, Fungal Mycelium and Cellulose Nanofibrils.
30842558	1	1	theme	fungal	136:141	arg1	mycelium					143:150	fungal mycelium	136:150	fungal mycelium	136:150	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	3	2	theme	wood	463:466	arg1	particles					468:476	unmodified wood particles	452:476	unmodified wood particles mixed with CNF and pure mycelium tissue	452:516	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	1	3	theme	present	224:230	arg1	study					232:236	the present study	220:236	the present study	220:236	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	7	4	with	addition	1301:1308	arg1	potential					1315:1323	potential	1315:1323	potential	1315:1323	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	6	5	theme	2.5	1119:1121	arg1	%					1122:1122	%	1122:1122	%	1122:1122	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	2	6	theme	experiments	253:263	arg1	set					246:248	one set	242:248	one set of experiments	242:263	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	2	6	theme	experiments	253:263	arg1	experiments					253:263	experiments	253:263	experiments	253:263	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	6	7	theme	elasticity	1094:1103	arg1	modulus					1060:1066	the modulus	1056:1066	the modulus of rupture and modulus of elasticity	1056:1103	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	3	8	theme	pure	497:500	arg1	tissue					511:516	pure mycelium tissue	497:516	pure mycelium tissue	497:516	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	3	9	theme	unmodified	452:461	arg1	particles					468:476	unmodified wood particles	452:476	unmodified wood particles mixed with CNF and pure mycelium tissue	452:516	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	6	10	theme	Mycelium	914:921	arg1	modification					923:934	Mycelium modification	914:934	Mycelium modification	914:934	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	5	11	theme	hyphae	843:848	arg1	network					825:831	a network	823:831	a network of fungal hyphae	823:848	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	6	12	theme	hybrid	1020:1025	arg1	composites					1027:1036	the hybrid composites	1016:1036	the hybrid composites	1016:1036	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	4	13	theme	enhanced	603:610	arg1	properties					636:645	enhanced physical and mechanical properties	603:645	enhanced physical and mechanical properties	603:645	It was found that the composites made of mycelium-modified wood and CNF resulted in enhanced physical and mechanical properties compared to the ones made by physically mixing wood, mycelium, and CNF.
30842558	5	14	theme	fungal	836:841	arg1	hyphae					843:848	fungal hyphae	836:848	fungal hyphae	836:848	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	7	15	theme	furniture	1353:1361	arg1	applications					1363:1374	furniture applications	1353:1374	furniture applications	1353:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	6	16	theme	composites	1027:1036	arg1	absorption					979:988	water absorption	973:988	water absorption	973:988	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	6	16	theme	composites	1027:1036	arg1	swelling					1004:1011	thickness swelling	994:1011	thickness swelling	994:1011	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	5	17	theme	wood	803:806	arg1	particles					808:816	wood particles	803:816	wood particles	803:816	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	0	18	theme	Bio-Based	6:14	arg1	Composites					23:32	Fully Bio-Based Hybrid Composites	0:32	Fully Bio-Based Hybrid Composites	0:32	Fully Bio-Based Hybrid Composites Made of Wood, Fungal Mycelium and Cellulose Nanofibrils.
30842558	7	19	theme	composite	1223:1231	arg1	systems					1233:1239	unique lightweight composite systems	1204:1239	unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1204:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	5	20	theme	mycelial	879:886	arg1	film					888:891	a uniform mycelial film	869:891	a uniform mycelial film	869:891	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	1	21	theme	cellulose	157:165	arg1	CNF					180:182	CNF	180:182	CNF	180:182	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	1	21	theme	cellulose	157:165	arg1	nanofibrils					167:177	cellulose nanofibrils	157:177	cellulose nanofibrils (CNF)	157:183	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	5	22	theme	uniform	871:877	arg1	film					888:891	a uniform mycelial film	869:891	a uniform mycelial film	869:891	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	4	23	theme	physical	612:619	arg1	properties					636:645	enhanced physical and mechanical properties	603:645	enhanced physical and mechanical properties	603:645	It was found that the composites made of mycelium-modified wood and CNF resulted in enhanced physical and mechanical properties compared to the ones made by physically mixing wood, mycelium, and CNF.
30842558	7	24	theme	5	1294:1294	arg1	%					1295:1295	%	1295:1295	%	1295:1295	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	3	25	theme	mycelium	502:509	arg1	tissue					511:516	pure mycelium tissue	497:516	pure mycelium tissue	497:516	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	6	26	theme	thickness	994:1002	arg1	swelling					1004:1011	thickness swelling	994:1011	thickness swelling	994:1011	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	2	27	theme	CNF	390:392	arg1	levels					380:385	various levels	372:385	various levels of CNF	372:392	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	7	28	with	systems	1233:1239	arg1	properties					1270:1279	physical and mechanical properties	1246:1279	physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1246:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	1	29	theme	hybrid	97:102	arg1	composites					110:119	Novel hybrid panel composites	91:119	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF)	91:183	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	7	30	theme	physical	1246:1253	arg1	properties					1270:1279	physical and mechanical properties	1246:1279	physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1246:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	7	31	theme	systems	1233:1239	arg1	development					1189:1199	the development	1185:1199	the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1185:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	7	32	theme	lightweight	1211:1221	arg1	systems					1233:1239	unique lightweight composite systems	1204:1239	unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1204:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	5	33	theme	mycelium	773:780	arg1	modification					782:793	mycelium modification	773:793	mycelium modification	773:793	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	7	34	theme	mechanical	1259:1268	arg1	properties					1270:1279	physical and mechanical properties	1246:1279	physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1246:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	2	35	theme	various	372:378	arg1	levels					380:385	various levels	372:385	various levels of CNF	372:392	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	5	36	theme	Scanning	719:726	arg1	SEM					749:751	SEM	749:751	SEM	749:751	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	5	36	theme	Scanning	719:726	arg1	microscopy					737:746	Scanning electron microscopy	719:746	Scanning electron microscopy (SEM) images	719:759	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	3	37	theme	other	409:413	arg1	experiments					422:432	experiments	422:432	experiments	422:432	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	3	37	theme	other	409:413	arg1	set					415:417	The other set	405:417	The other set of experiments	405:432	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	5	38	theme	electron	728:735	arg1	SEM					749:751	SEM	749:751	SEM	749:751	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	5	38	theme	electron	728:735	arg1	microscopy					737:746	Scanning electron microscopy	719:746	Scanning electron microscopy (SEM) images	719:759	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	6	39	theme	water	973:977	arg1	absorption					979:988	water absorption	973:988	water absorption	973:988	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	5	40	theme	wood	898:901	arg1	particles					903:911	wood particles	898:911	wood particles	898:911	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	1	41	theme	panel	104:108	arg1	composites					110:119	Novel hybrid panel composites	91:119	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF)	91:183	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	5	42	theme	microscopy	737:746	arg1	images					754:759	Scanning electron microscopy (SEM) images	719:759	Scanning electron microscopy (SEM) images	719:759	Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles.
30842558	6	43	theme	modulus	1083:1089	arg1	modulus					1060:1066	the modulus	1056:1066	the modulus of rupture and modulus of elasticity	1056:1103	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	7	44	theme	unique	1204:1209	arg1	systems					1233:1239	unique lightweight composite systems	1204:1239	unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications	1204:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	2	45	theme	softwood	288:295	arg1	particles					297:305	softwood particles	288:305	softwood particles	288:305	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	6	46	theme	%	1122:1122	arg1	addition					1124:1131	2.5% addition	1119:1131	2.5% addition	1119:1131	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	7	47	theme	%	1295:1295	arg1	addition					1301:1308	5% CNF addition	1294:1308	5% CNF addition with potential to be used in packaging and furniture applications	1294:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	3	48	theme	experiments	422:432	arg1	experiments					422:432	experiments	422:432	experiments	422:432	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	3	48	theme	experiments	422:432	arg1	set					415:417	The other set	405:417	The other set of experiments	405:432	The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue.
30842558	1	49	theme	Novel	91:95	arg1	composites					110:119	Novel hybrid panel composites	91:119	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF)	91:183	Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study.
30842558	7	50	theme	CNF	1297:1299	arg1	addition					1301:1308	5% CNF addition	1294:1308	5% CNF addition with potential to be used in packaging and furniture applications	1294:1374	We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.
30842558	4	51	theme	mechanical	625:634	arg1	properties					636:645	enhanced physical and mechanical properties	603:645	enhanced physical and mechanical properties	603:645	It was found that the composites made of mycelium-modified wood and CNF resulted in enhanced physical and mechanical properties compared to the ones made by physically mixing wood, mycelium, and CNF.
30842558	6	52	theme	rupture	1071:1077	arg1	modulus					1060:1066	the modulus	1056:1066	the modulus of rupture and modulus of elasticity	1056:1103	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	6	53	contain	had	936:938	arg2	effect					954:959	a significant effect	940:959	a significant effect	940:959	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	6	53	contain	had	936:938	arg1	modification					923:934	Mycelium modification	914:934	Mycelium modification	914:934	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
30842558	4	54	theme	mycelium-modified	560:576	arg1	wood					578:581	mycelium-modified wood	560:581	mycelium-modified wood	560:581	It was found that the composites made of mycelium-modified wood and CNF resulted in enhanced physical and mechanical properties compared to the ones made by physically mixing wood, mycelium, and CNF.
30842558	0	55	theme	Fungal	48:53	arg1	Mycelium					55:62	Fungal Mycelium	48:62	Fungal Mycelium	48:62	Fully Bio-Based Hybrid Composites Made of Wood, Fungal Mycelium and Cellulose Nanofibrils.
30842558	0	56	theme	Hybrid	16:21	arg1	Composites					23:32	Fully Bio-Based Hybrid Composites	0:32	Fully Bio-Based Hybrid Composites	0:32	Fully Bio-Based Hybrid Composites Made of Wood, Fungal Mycelium and Cellulose Nanofibrils.
30842558	2	57	theme	mycelium-modified	318:334	arg1	binder					397:402	binder	397:402	binder	397:402	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	2	57	theme	mycelium-modified	318:334	arg1	wood					336:339	mycelium-modified wood	318:339	mycelium-modified wood which was then hybridized with various levels of CNF as binder	318:402	In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder.
30842558	6	58	theme	significant	942:952	arg1	effect					954:959	a significant effect	940:959	a significant effect	940:959	Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition.
31195764	0	0	theme	lactuca	85:91	arg1	Extracts					68:75	the Sulfated Polysaccharides Extracts	39:75	the Sulfated Polysaccharides Extracts of Ulva lactuca	39:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	1	1	theme	algae	375:379	arg1	extracts					353:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts	288:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	1	2	theme	aqueous	292:298	arg1	weight					315:320	the aqueous high molecular weight	288:320	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	6	3	theme	remarkable	955:964	arg1	activity					976:983	the remarkable antitumor activity	951:983	the remarkable antitumor activity of F5 on three types of cancer cell lines	951:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	8	4	theme	groups	1461:1466	arg1	DP					1406:1407	DP	1406:1407	DP	1406:1407	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	4	theme	groups	1461:1466	arg1	composition					1410:1420	composition	1410:1420	composition	1410:1420	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	4	theme	groups	1461:1466	arg1	type					1427:1430	type	1427:1430	type	1427:1430	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	2	5	theme	different	512:520	arg1	F1-F8					550:554	F1-F8	550:554	F1-F8	550:554	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	2	5	theme	different	512:520	arg1	fractions					539:547	eight different molecular weight fractions	506:547	eight different molecular weight fractions (F1-F8) using ion exchange chromatography	506:589	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	0	6	dep	Followed	131:138	arg1	Production					0:9	Production	0:9	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca	0:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	1	7	theme	high	300:303	arg1	weight					315:320	the aqueous high molecular weight	288:320	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	0	8	theme	Post-Extraction	94:108	arg1	Hydrolysis					120:129	Post-Extraction Enzymatic Hydrolysis	94:129	Post-Extraction Enzymatic Hydrolysis	94:129	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	8	9	theme	functional	1450:1459	arg1	groups					1461:1466	characteristic functional groups	1435:1466	characteristic functional groups	1435:1466	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	7	10	theme	cooperativity	1337:1349	arg1	degree					1327:1332	a higher degree	1318:1332	a higher degree of cooperativity	1318:1349	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	1	11	theme	molecular	305:313	arg1	weight					315:320	the aqueous high molecular weight	288:320	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	9	12	theme	work	1514:1517	arg1	implications					1493:1504	The implications	1489:1504	The implications of this work	1489:1517	The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.
31195764	6	13	theme	sulfate	1104:1110	arg1	contents					1065:1072	its comparable contents	1050:1072	its comparable contents	1050:1072	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	1	14	theme	weight	315:320	arg1	extracts					353:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts	288:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	6	15	dep	contents	1143:1150	arg1	addition					1116:1123	addition	1116:1123	addition	1116:1123	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	7	16	theme	ligand	1354:1359	arg1	binding					1361:1367	ligand binding	1354:1367	ligand binding	1354:1367	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	7	17	theme	highest	1247:1253	arg1	coefficient					1260:1270	the highest Hill coefficient	1243:1270	the highest Hill coefficient among the four promising fractions	1243:1305	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	4	18	dep	Fourier	795:801	arg1	Transform					803:811	Transform	803:811	Transform Infrared Spectroscopy (FTIR)	803:840	In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR).
31195764	0	19	theme	Enzymatic	110:118	arg1	Hydrolysis					120:129	Post-Extraction Enzymatic Hydrolysis	94:129	Post-Extraction Enzymatic Hydrolysis	94:129	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	7	20	contain	possessed	1233:1241	arg1	F5					1225:1226	F5	1225:1226	F5	1225:1226	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	7	20	contain	possessed	1233:1241	arg2	coefficient					1260:1270	the highest Hill coefficient	1243:1270	the highest Hill coefficient among the four promising fractions	1243:1305	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	6	21	from	activity	976:983	arg1	types					1000:1004	three types	994:1004	three types of cancer cell lines	994:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	5	22	theme	antitumor	905:913	arg1	activities					915:924	promising antioxidant and antitumor activities	879:924	promising antioxidant and antitumor activities	879:924	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	1	23	theme	novel	210:214	arg1	process					241:247	a novel combined post-extraction process	208:247	a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca	208:393	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	2	24	theme	ion	563:565	arg1	chromatography					576:589	ion exchange chromatography	563:589	ion exchange chromatography	563:589	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	1	25	theme	sulfated	322:329	arg1	SPs					348:350	SPs	348:350	SPs	348:350	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	1	25	theme	sulfated	322:329	arg1	polysaccharides					331:345	sulfated polysaccharides	322:345	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	4	26	theme	polymerization	730:743	arg1	degree					720:725	their degree	714:725	their degree of polymerization (DP)	714:748	In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR).
31195764	1	27	theme	combined	216:223	arg1	process					241:247	a novel combined post-extraction process	208:247	a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca	208:393	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	0	28	theme	Compounds	24:32	arg1	Production					0:9	Production	0:9	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca	0:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	1	29	theme	polysaccharides	331:345	arg1	extracts					353:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts	288:360	the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae	288:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	7	30	theme	higher	1320:1325	arg1	degree					1327:1332	a higher degree	1318:1332	a higher degree of cooperativity	1318:1349	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	1	31	theme	post-extraction	225:239	arg1	process					241:247	a novel combined post-extraction process	208:247	a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca	208:393	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	0	32	theme	Bioactive	14:22	arg1	Compounds					24:32	Bioactive Compounds	14:32	Bioactive Compounds	14:32	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	2	33	theme	exchange	567:574	arg1	chromatography					576:589	ion exchange chromatography	563:589	ion exchange chromatography	563:589	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	3	34	theme	Crude	592:596	arg1	SPs					598:600	Crude SPs	592:600	Crude SPs together with V45 and (F1-F8)	592:630	Crude SPs together with V45 and (F1-F8) were examined for their carbohydrate, protein, and sulfate contents.
31195764	6	35	theme	cancer	1009:1014	arg1	lines					1021:1025	cancer cell lines	1009:1025	cancer cell lines	1009:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	0	36	theme	Chromatographic	156:170	arg1	Fractionation					172:184	Ion-Exchange Chromatographic Fractionation	143:184	Ion-Exchange Chromatographic Fractionation	143:184	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	4	37	theme	Infrared	813:820	arg1	FTIR					836:839	FTIR	836:839	FTIR	836:839	In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR).
31195764	4	37	theme	Infrared	813:820	arg1	Spectroscopy					822:833	Infrared Spectroscopy	813:833	Infrared Spectroscopy (FTIR)	813:840	In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR).
31195764	6	38	theme	lines	1021:1025	arg1	types					1000:1004	three types	994:1004	three types of cancer cell lines	994:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	9	39	theme	pharmaceuticals	1584:1598	arg1	industries					1549:1558	the industries	1545:1558	the industries of food supplements and pharmaceuticals	1545:1598	The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.
31195764	0	40	theme	Ion-Exchange	143:154	arg1	Fractionation					172:184	Ion-Exchange Chromatographic Fractionation	143:184	Ion-Exchange Chromatographic Fractionation	143:184	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	6	41	theme	antitumor	966:974	arg1	activity					976:983	the remarkable antitumor activity	951:983	the remarkable antitumor activity of F5 on three types of cancer cell lines	951:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	6	42	theme	acid	1179:1182	arg1	contents					1143:1150	its comparable contents	1128:1150	its comparable contents of rhamnose and glucuronic acid	1128:1182	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	0	43	theme	Polysaccharides	52:66	arg1	Extracts					68:75	the Sulfated Polysaccharides Extracts	39:75	the Sulfated Polysaccharides Extracts of Ulva lactuca	39:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	7	44	theme	promising	1287:1295	arg1	fractions					1297:1305	the four promising fractions	1278:1305	the four promising fractions	1278:1305	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	0	45	from	Extracts	68:75	arg1	Production					0:9	Production	0:9	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca	0:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	6	46	theme	glucuronic	1168:1177	arg1	acid					1179:1182	glucuronic acid	1168:1182	glucuronic acid	1168:1182	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	8	47	theme	characteristic	1435:1448	arg1	groups					1461:1466	characteristic functional groups	1435:1466	characteristic functional groups	1435:1466	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	6	48	theme	carbohydrate	1086:1097	arg1	contents					1065:1072	its comparable contents	1050:1072	its comparable contents	1050:1072	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	0	49	theme	Sulfated	43:50	arg1	Extracts					68:75	the Sulfated Polysaccharides Extracts	39:75	the Sulfated Polysaccharides Extracts of Ulva lactuca	39:91	Production of Bioactive Compounds from the Sulfated Polysaccharides Extracts of Ulva lactuca: Post-Extraction Enzymatic Hydrolysis Followed by Ion-Exchange Chromatographic Fractionation.
31195764	7	50	theme	Hill	1255:1258	arg1	coefficient					1260:1270	the highest Hill coefficient	1243:1270	the highest Hill coefficient among the four promising fractions	1243:1305	F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding.
31195764	9	51	theme	food	1563:1566	arg1	industries					1549:1558	the industries	1545:1558	the industries of food supplements and pharmaceuticals	1545:1598	The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.
31195764	5	52	theme	promising	879:887	arg1	activities					915:924	promising antioxidant and antitumor activities	879:924	promising antioxidant and antitumor activities	879:924	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	8	53	theme	Other	1370:1374	arg1	DP					1406:1407	DP	1406:1407	DP	1406:1407	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	53	theme	Other	1370:1374	arg1	factors					1388:1394	Other influencing factors	1370:1394	Other influencing factors including DP, composition, and type of characteristic functional groups	1370:1466	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	53	theme	Other	1370:1374	arg1	composition					1410:1420	composition	1410:1420	composition	1410:1420	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	53	theme	Other	1370:1374	arg1	type					1427:1430	type	1427:1430	type	1427:1430	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	3	54	theme	sulfate	683:689	arg1	contents					691:698	sulfate contents	683:698	sulfate contents	683:698	Crude SPs together with V45 and (F1-F8) were examined for their carbohydrate, protein, and sulfate contents.
31195764	6	55	theme	rhamnose	1155:1162	arg1	contents					1143:1150	its comparable contents	1128:1150	its comparable contents of rhamnose and glucuronic acid	1128:1182	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	5	56	theme	antioxidant	889:899	arg1	activities					915:924	promising antioxidant and antitumor activities	879:924	promising antioxidant and antitumor activities	879:924	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	1	57	theme	bioactive	263:271	arg1	compounds					273:281	bioactive compounds	263:281	bioactive compounds	263:281	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	5	58	dep	Fractions	843:851	arg1	Fractions					843:851	Fractions S1, F4, F5, and F8	843:870	Fractions S1, F4, F5, and F8	843:870	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	5	58	dep	Fractions	843:851	arg1	F5					861:862	F5	861:862	F5	861:862	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	5	58	dep	Fractions	843:851	arg1	F8					869:870	F8	869:870	F8	869:870	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	5	58	dep	Fractions	843:851	arg1	F4					857:858	F4	857:858	F4	857:858	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	5	58	dep	Fractions	843:851	arg1	S1					853:854	S1	853:854	S1	853:854	Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro.
31195764	6	59	theme	protein	1077:1083	arg1	contents					1065:1072	its comparable contents	1050:1072	its comparable contents	1050:1072	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	2	60	theme	weight	532:537	arg1	F1-F8					550:554	F1-F8	550:554	F1-F8	550:554	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	2	60	theme	weight	532:537	arg1	fractions					539:547	eight different molecular weight fractions	506:547	eight different molecular weight fractions (F1-F8) using ion exchange chromatography	506:589	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	8	61	theme	influencing	1376:1386	arg1	DP					1406:1407	DP	1406:1407	DP	1406:1407	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	61	theme	influencing	1376:1386	arg1	factors					1388:1394	Other influencing factors	1370:1394	Other influencing factors including DP, composition, and type of characteristic functional groups	1370:1466	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	61	theme	influencing	1376:1386	arg1	composition					1410:1420	composition	1410:1420	composition	1410:1420	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	8	61	theme	influencing	1376:1386	arg1	type					1427:1430	type	1427:1430	type	1427:1430	Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed.
31195764	6	62	theme	cell	1016:1019	arg1	lines					1021:1025	cancer cell lines	1009:1025	cancer cell lines	1009:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	9	63	dep	food	1563:1566	arg1	supplements					1568:1578	supplements	1568:1578	supplements	1568:1578	The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.
31195764	6	64	theme	F5	988:989	arg1	activity					976:983	the remarkable antitumor activity	951:983	the remarkable antitumor activity of F5 on three types of cancer cell lines	951:1025	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	2	65	theme	molecular	522:530	arg1	F1-F8					550:554	F1-F8	550:554	F1-F8	550:554	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	2	65	theme	molecular	522:530	arg1	fractions					539:547	eight different molecular weight fractions	506:547	eight different molecular weight fractions (F1-F8) using ion exchange chromatography	506:589	After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1-F8) using ion exchange chromatography.
31195764	1	66	theme	green	369:373	arg1	algae					375:379	the green algae	365:379	the green algae	365:379	This paper describes a novel combined post-extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca.
31195764	6	67	theme	comparable	1132:1141	arg1	contents					1143:1150	its comparable contents	1128:1150	its comparable contents of rhamnose and glucuronic acid	1128:1182	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31195764	6	68	theme	comparable	1054:1063	arg1	contents					1065:1072	its comparable contents	1050:1072	its comparable contents	1050:1072	In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose.
31751727	0	0	theme	metals	94:99	arg1	removal					77:83	removal	77:83	removal of heavy metals	77:99	Electrospun cellulose acetate nanofiber incorporated with hydroxyapatite for removal of heavy metals.
31751727	6	1	theme	various	888:894	arg1	conditions					907:916	various adsorption conditions	888:916	various adsorption conditions	888:916	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	4	2	theme	uniform	645:651	arg1	nanofiber					667:675	uniform and bead-free nanofiber	645:675	uniform and bead-free nanofiber	645:675	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	5	3	from	variations	835:844	arg1	morphology					859:868	the fiber morphology	849:868	the fiber morphology	849:868	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	1	4	theme	ions	148:151	arg1	impact					122:127	the negative impact	109:127	the negative impact of the heavy metal ions in water	109:160	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	1	5	theme	urgent	216:221	arg1	requests					223:230	the urgent requests	212:230	the urgent requests for wastewater treatment	212:255	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	0	6	theme	heavy	88:92	arg1	metals					94:99	heavy metals	88:99	heavy metals	88:99	Electrospun cellulose acetate nanofiber incorporated with hydroxyapatite for removal of heavy metals.
31751727	2	7	theme	organic/inorganic	323:339	arg1	composites					367:376	new organic/inorganic hybrid nanofiber membrane composites	319:376	new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique	319:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	1	8	theme	requests	223:230	arg1	requests					223:230	the urgent requests	212:230	the urgent requests for wastewater treatment	212:255	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	1	8	theme	requests	223:230	arg1	rejection					167:175	the rejection	163:175	the rejection of these toxic materials	163:200	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	1	8	theme	requests	223:230	arg1	one					205:207	one	205:207	one	205:207	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	3	9	with	impregnated	516:526	arg1	hydroxyapatite					533:546	hydroxyapatite	533:546	hydroxyapatite (CA/HAp)	533:555	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	9	with	impregnated	516:526	arg1	CA/HAp					549:554	CA/HAp	549:554	CA/HAp	549:554	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	10	theme	pure	465:468	arg1	nanofibers					488:497	pure cellulose acetate nanofibers	465:497	pure cellulose acetate nanofibers (CA NFs)	465:506	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	10	theme	pure	465:468	arg1	NFs					503:505	CA NFs	500:505	CA NFs	500:505	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	11	theme	CA	500:501	arg1	nanofibers					488:497	pure cellulose acetate nanofibers	465:497	pure cellulose acetate nanofibers (CA NFs)	465:506	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	11	theme	CA	500:501	arg1	NFs					503:505	CA NFs	500:505	CA NFs	500:505	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	10	12	theme	surface	1327:1333	arg1	complexation					1335:1346	surface complexation	1327:1346	surface complexation	1327:1346	The adsorption mechanism on the CA/HAp composite can be established via ion exchange and surface complexation.
31751727	2	13	theme	hybrid	341:346	arg1	composites					367:376	new organic/inorganic hybrid nanofiber membrane composites	319:376	new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique	319:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	1	14	theme	wastewater	236:245	arg1	treatment					247:255	wastewater treatment	236:255	wastewater treatment	236:255	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	8	15	theme	nanofiber	1104:1112	arg1	composite					1114:1122	hybrid nanofiber composite	1097:1122	hybrid nanofiber composite	1097:1122	for adsorption Pb (II) and Fe (III) ions using hybrid nanofiber composite, respectively are obtained.
31751727	2	16	theme	new	319:321	arg1	composites					367:376	new organic/inorganic hybrid nanofiber membrane composites	319:376	new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique	319:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	5	17	theme	slight	770:775	arg1	changes					777:783	the slight changes	766:783	the slight changes in operating parameters	766:807	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	3	18	theme	acetate	480:486	arg1	nanofibers					488:497	pure cellulose acetate nanofibers	465:497	pure cellulose acetate nanofibers (CA NFs)	465:506	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	18	theme	acetate	480:486	arg1	NFs					503:505	CA NFs	500:505	CA NFs	500:505	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	5	19	theme	significant	823:833	arg1	variations					835:844	significant variations	823:844	significant variations in the fiber morphology	823:868	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	8	20	theme	hybrid	1097:1102	arg1	composite					1114:1122	hybrid nanofiber composite	1097:1122	hybrid nanofiber composite	1097:1122	for adsorption Pb (II) and Fe (III) ions using hybrid nanofiber composite, respectively are obtained.
31751727	9	21	dep	pseudo-second-order	1195:1213	arg1	models					1230:1235	models	1230:1235	models	1230:1235	The adsorption process was found to obey a pseudo-second-order and Freundlich models.
31751727	3	22	theme	nanofibers	488:497	arg1	manufacturing					448:460	The manufacturing	444:460	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	444:576	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	0	23	theme	cellulose	12:20	arg1	nanofiber					30:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber incorporated with hydroxyapatite for removal of heavy metals.
31751727	2	24	theme	batch	427:431	arg1	technique					433:441	a batch technique	425:441	a batch technique	425:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	0	25	theme	Electrospun	0:10	arg1	nanofiber					30:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber incorporated with hydroxyapatite for removal of heavy metals.
31751727	4	26	theme	nanofiber	667:675	arg1	process					634:640	A production process	621:640	A production process of uniform and bead-free nanofiber	621:675	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	9	27	theme	adsorption	1156:1165	arg1	process					1167:1173	The adsorption process	1152:1173	The adsorption process	1152:1173	The adsorption process was found to obey a pseudo-second-order and Freundlich models.
31751727	2	28	theme	facile	276:281	arg1	fabrication					283:293	facile fabrication	276:293	facile fabrication	276:293	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	2	29	theme	composites	367:376	arg1	characterization					299:314	characterization	299:314	characterization	299:314	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	2	29	theme	composites	367:376	arg1	fabrication					283:293	facile fabrication	276:293	facile fabrication	276:293	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	2	30	theme	Pb	406:407	arg1	ions					414:417	Fe (III) and Pb (II) ions	393:417	Fe (III) and Pb (II) ions using a batch technique	393:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	6	31	theme	removal	940:946	arg1	efficiency					948:957	the removal efficiency	936:957	the removal efficiency	936:957	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	1	32	from	impact	122:127	arg1	water					156:160	water	156:160	water	156:160	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	4	33	theme	various	703:709	arg1	conditions					727:736	various electrospinning conditions	703:736	various electrospinning conditions	703:736	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	0	34	theme	acetate	22:28	arg1	nanofiber					30:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber	0:38	Electrospun cellulose acetate nanofiber incorporated with hydroxyapatite for removal of heavy metals.
31751727	1	35	theme	heavy	136:140	arg1	ions					148:151	the heavy metal ions	132:151	the heavy metal ions	132:151	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	5	36	from	changes	777:783	arg1	parameters					798:807	operating parameters	788:807	operating parameters	788:807	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	10	37	theme	adsorption	1242:1251	arg1	mechanism					1253:1261	The adsorption mechanism	1238:1261	The adsorption mechanism on the CA/HAp composite	1238:1285	The adsorption mechanism on the CA/HAp composite can be established via ion exchange and surface complexation.
31751727	1	38	theme	toxic	186:190	arg1	materials					192:200	these toxic materials	180:200	these toxic materials	180:200	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	10	39	theme	ion	1310:1312	arg1	exchange					1314:1321	ion exchange	1310:1321	ion exchange	1310:1321	The adsorption mechanism on the CA/HAp composite can be established via ion exchange and surface complexation.
31751727	8	40	theme	adsorption	1054:1063	arg1	II					1069:1070	II	1069:1070	II	1069:1070	for adsorption Pb (II) and Fe (III) ions using hybrid nanofiber composite, respectively are obtained.
31751727	8	40	theme	adsorption	1054:1063	arg1	Pb					1065:1066	adsorption Pb	1054:1066	adsorption Pb (II)	1054:1071	for adsorption Pb (II) and Fe (III) ions using hybrid nanofiber composite, respectively are obtained.
31751727	4	41	theme	electrospinning	711:725	arg1	conditions					727:736	various electrospinning conditions	703:736	various electrospinning conditions	703:736	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	1	42	theme	materials	192:200	arg1	requests					223:230	the urgent requests	212:230	the urgent requests for wastewater treatment	212:255	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	1	42	theme	materials	192:200	arg1	rejection					167:175	the rejection	163:175	the rejection of these toxic materials	163:200	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	1	42	theme	materials	192:200	arg1	one					205:207	one	205:207	one	205:207	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	2	43	theme	ions	414:417	arg1	removal					382:388	removal	382:388	removal of Fe (III) and Pb (II) ions using a batch technique	382:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	5	44	theme	fiber	853:857	arg1	morphology					859:868	the fiber morphology	849:868	the fiber morphology	849:868	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	3	45	theme	impregnated	516:526	arg1	fibers					571:576	its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	512:576	its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	512:576	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	6	46	from	effect	926:931	arg1	efficiency					948:957	the removal efficiency	936:957	the removal efficiency	936:957	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	1	47	theme	negative	113:120	arg1	impact					122:127	the negative impact	109:127	the negative impact of the heavy metal ions in water	109:160	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	7	48	theme	%	1026:1026	arg1	efficiency					992:1001	High separation efficiency	976:1001	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.	976:1048	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.
31751727	7	49	theme	separation	981:990	arg1	efficiency					992:1001	High separation efficiency	976:1001	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.	976:1048	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.
31751727	6	50	theme	conditions	907:916	arg1	effect					926:931	its effect	922:931	its effect on the removal efficiency	922:957	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	6	50	theme	conditions	907:916	arg1	influence					875:883	The influence	871:883	The influence of various adsorption conditions	871:916	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	4	51	theme	bead-free	657:665	arg1	nanofiber					667:675	uniform and bead-free nanofiber	645:675	uniform and bead-free nanofiber	645:675	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	10	52	from	mechanism	1253:1261	arg1	composite					1277:1285	the CA/HAp composite	1266:1285	the CA/HAp composite	1266:1285	The adsorption mechanism on the CA/HAp composite can be established via ion exchange and surface complexation.
31751727	3	53	theme	nanocomposite	557:569	arg1	fibers					571:576	its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	512:576	its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	512:576	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	4	54	theme	production	623:632	arg1	process					634:640	A production process	621:640	A production process of uniform and bead-free nanofiber	621:675	A production process of uniform and bead-free nanofiber is developed by adjusting various electrospinning conditions.
31751727	10	55	theme	CA/HAp	1270:1275	arg1	composite					1277:1285	the CA/HAp composite	1266:1285	the CA/HAp composite	1266:1285	The adsorption mechanism on the CA/HAp composite can be established via ion exchange and surface complexation.
31751727	3	56	theme	electrospinning	596:610	arg1	process					612:618	an electrospinning process	593:618	an electrospinning process	593:618	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	57	theme	fibers	571:576	arg1	manufacturing					448:460	The manufacturing	444:460	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers	444:576	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	1	58	theme	metal	142:146	arg1	ions					148:151	the heavy metal ions	132:151	the heavy metal ions	132:151	Due to the negative impact of the heavy metal ions in water, the rejection of these toxic materials is one of the urgent requests for wastewater treatment.
31751727	2	59	theme	Fe	393:394	arg1	ions					414:417	Fe (III) and Pb (II) ions	393:417	Fe (III) and Pb (II) ions using a batch technique	393:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	7	60	theme	High	976:979	arg1	efficiency					992:1001	High separation efficiency	976:1001	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.	976:1048	High separation efficiency of about 99.7 and 95.46% within 35 and 40 min.
31751727	2	61	theme	membrane	358:365	arg1	composites					367:376	new organic/inorganic hybrid nanofiber membrane composites	319:376	new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique	319:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
31751727	3	62	theme	cellulose	470:478	arg1	nanofibers					488:497	pure cellulose acetate nanofibers	465:497	pure cellulose acetate nanofibers (CA NFs)	465:506	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	3	62	theme	cellulose	470:478	arg1	NFs					503:505	CA NFs	500:505	CA NFs	500:505	The manufacturing of pure cellulose acetate nanofibers (CA NFs) and its impregnated with hydroxyapatite (CA/HAp) nanocomposite fibers is explored by an electrospinning process.
31751727	6	63	from	influence	875:883	arg1	efficiency					948:957	the removal efficiency	936:957	the removal efficiency	936:957	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	6	64	theme	adsorption	896:905	arg1	conditions					907:916	various adsorption conditions	888:916	various adsorption conditions	888:916	The influence of various adsorption conditions and its effect on the removal efficiency is investigated.
31751727	5	65	theme	operating	788:796	arg1	parameters					798:807	operating parameters	788:807	operating parameters	788:807	The experiments prove that the slight changes in operating parameters may result in significant variations in the fiber morphology.
31751727	2	66	theme	nanofiber	348:356	arg1	composites					367:376	new organic/inorganic hybrid nanofiber membrane composites	319:376	new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique	319:441	This work aims to facile fabrication and characterization of new organic/inorganic hybrid nanofiber membrane composites for removal of Fe (III) and Pb (II) ions using a batch technique.
30718663	2	0	theme	physical	439:446	arg1	properties					448:457	their physical properties	433:457	their physical properties	433:457	BC dressings were thoroughly analyzed with regard to their physical properties.
30718663	7	1	theme	eradication	1141:1151	arg1	ability					1153:1159	the highest eradication ability	1129:1159	the highest eradication ability	1129:1159	The clove EO displayed the highest eradication ability toward staphylococcal, while the thyme EO against pseudomonal biofilm.
30718663	6	2	theme	cell	1094:1097	arg1	lines					1099:1103	osteoblast cell lines	1083:1103	osteoblast cell lines	1083:1103	The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines.
30718663	9	3	theme	eucalyptus	1576:1585	arg1	potential					1626:1634	of highest potential	1615:1634	of highest potential	1615:1634	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	3	theme	eucalyptus	1576:1585	arg1	dressing					1603:1610	the eucalyptus EO-saturated BC dressing	1572:1610	the eucalyptus EO-saturated BC dressing	1572:1610	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	8	4	theme	immune	1456:1461	arg1	system					1463:1468	immune system effector cells	1456:1483	immune system effector cells	1456:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	9	5	theme	EO-saturated	1587:1598	arg1	potential					1626:1634	of highest potential	1615:1634	of highest potential	1615:1634	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	5	theme	EO-saturated	1587:1598	arg1	dressing					1603:1610	the eucalyptus EO-saturated BC dressing	1572:1610	the eucalyptus EO-saturated BC dressing	1572:1610	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	5	6	theme	compounds	915:923	arg1	number					905:910	the highest number	893:910	the highest number of compounds able to adhere to HA	893:944	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	4	7	theme	species	712:718	arg1	production					726:735	reactive oxygen species (ROS) production	696:735	reactive oxygen species (ROS) production by macrophages	696:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	5	8	located	found	950:954	arg2	number					905:910	the highest number	893:910	the highest number of compounds able to adhere to HA	893:944	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	5	8	located	found	950:954	arg1	EO					969:970	the thyme EO	959:970	the thyme EO	959:970	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	4	9	theme	oxygen	705:710	arg1	species					712:718	reactive oxygen species	696:718	reactive oxygen species (ROS) production by macrophages	696:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	4	9	theme	oxygen	705:710	arg1	ROS					721:723	ROS	721:723	ROS	721:723	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	9	10	theme	BC	1600:1601	arg1	potential					1626:1634	of highest potential	1615:1634	of highest potential	1615:1634	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	10	theme	BC	1600:1601	arg1	dressing					1603:1610	the eucalyptus EO-saturated BC dressing	1572:1610	the eucalyptus EO-saturated BC dressing	1572:1610	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	5	11	theme	able	925:928	arg1	compounds					915:923	compounds	915:923	compounds able to adhere to HA	915:944	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	1	12	theme	staphylococcal	309:322	arg1	biofilms					340:347	staphylococcal and pseudomonal biofilms	309:347	staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA)	309:377	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	6	13	theme	eucalyptus	977:986	arg1	EO					988:989	The eucalyptus EO	973:989	The eucalyptus EO	973:989	The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines.
30718663	8	14	theme	EO-saturated	1297:1308	arg1	dressings					1313:1321	EO-saturated BC dressings	1297:1321	EO-saturated BC dressings	1297:1321	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	4	15	theme	dressings	623:631	arg1	cytotoxicity					575:586	cytotoxicity	575:586	cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages	575:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	4	15	theme	dressings	623:631	arg1	impact					686:691	their impact	680:691	cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages	575:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	8	16	dep	system	1463:1468	arg1	cells					1479:1483	effector cells	1470:1483	immune system effector cells	1456:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	1	17	from	research	126:133	arg1	saturated					225:233	saturated	225:233	saturated	225:233	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	9	18	theme	cytotoxic	1502:1510	arg1	effects					1512:1518	cytotoxic effects	1502:1518	cytotoxic effects of thyme and clove EOs towards cell lines in vitro	1502:1569	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	1	19	theme	pseudomonal	328:338	arg1	biofilms					340:347	staphylococcal and pseudomonal biofilms	309:347	staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA)	309:377	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	9	20	theme	of	1615:1616	arg1	potential					1626:1634	of highest potential	1615:1634	of highest potential	1615:1634	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	20	theme	of	1615:1616	arg1	dressing					1603:1610	the eucalyptus EO-saturated BC dressing	1572:1610	the eucalyptus EO-saturated BC dressing	1572:1610	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	3	21	theme	molecules	521:529	arg1	ability					496:502	ability	496:502	ability of particular EO molecules to adhere to HA	496:545	Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed.
30718663	3	21	theme	molecules	521:529	arg1	composition					480:490	the exact composition	470:490	the exact composition	470:490	Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed.
30718663	8	22	theme	effector	1470:1477	arg1	cells					1479:1483	effector cells	1470:1483	immune system effector cells	1456:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	7	23	theme	clove	1110:1114	arg1	EO					1116:1117	The clove EO	1106:1117	The clove EO	1106:1117	The clove EO displayed the highest eradication ability toward staphylococcal, while the thyme EO against pseudomonal biofilm.
30718663	8	24	theme	oxygen	1427:1432	arg1	species					1434:1440	reactive oxygen species	1418:1440	reactive oxygen species production by immune system effector cells	1418:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	4	25	theme	reactive	696:703	arg1	species					712:718	reactive oxygen species	696:718	reactive oxygen species (ROS) production by macrophages	696:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	4	25	theme	reactive	696:703	arg1	ROS					721:723	ROS	721:723	ROS	721:723	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	0	26	theme	Carrier	26:32	arg1	Potential					0:8	Potential	0:8	Potential of Biocellulose Carrier	0:32	Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite.
30718663	1	27	from	saturated	225:233	arg1	research					126:133	this research	121:133	this research	121:133	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	9	28	theme	cell	1551:1554	arg1	lines					1556:1560	cell lines	1551:1560	cell lines	1551:1560	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	3	29	theme	particular	507:516	arg1	molecules					521:529	particular EO molecules	507:529	particular EO molecules	507:529	Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed.
30718663	9	30	theme	clove	1533:1537	arg1	EOs					1539:1541	clove EOs	1533:1541	clove EOs	1533:1541	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	5	31	theme	high	791:794	arg1	ability					796:802	the high ability	787:802	the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure	787:890	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	2	32	theme	BC	380:381	arg1	dressings					383:391	BC dressings	380:391	BC dressings	380:391	BC dressings were thoroughly analyzed with regard to their physical properties.
30718663	5	33	theme	highest	897:903	arg1	number					905:910	the highest number	893:910	the highest number of compounds able to adhere to HA	893:944	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	3	34	theme	EO	518:519	arg1	molecules					521:529	particular EO molecules	507:529	particular EO molecules	507:529	Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed.
30718663	6	35	theme	osteoblast	1083:1092	arg1	lines					1099:1103	osteoblast cell lines	1083:1103	osteoblast cell lines	1083:1103	The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines.
30718663	5	36	theme	BC	807:808	arg1	dressings					810:818	BC dressings	807:818	BC dressings	807:818	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	9	37	theme	highest	1618:1624	arg1	potential					1626:1634	of highest potential	1615:1634	of highest potential	1615:1634	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	37	theme	highest	1618:1624	arg1	dressing					1603:1610	the eucalyptus EO-saturated BC dressing	1572:1610	the eucalyptus EO-saturated BC dressing	1572:1610	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	9	38	theme	EOs	1539:1541	arg1	effects					1512:1518	cytotoxic effects	1502:1518	cytotoxic effects of thyme and clove EOs towards cell lines in vitro	1502:1569	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	4	39	from	cytotoxicity	575:586	arg1	production					726:735	reactive oxygen species (ROS) production	696:735	reactive oxygen species (ROS) production by macrophages	696:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	1	40	theme	promising	178:186	arg1	biopolymers					188:198	the most promising biopolymers	169:198	the most promising biopolymers of the recent years	169:218	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	5	41	theme	dressings	810:818	arg1	ability					796:802	the high ability	787:802	the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure	787:890	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	1	42	theme	clove	262:266	arg1	EOs					284:286	EOs	284:286	EOs	284:286	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	42	theme	clove	262:266	arg1	oils					278:281	clove essential oils	262:281	clove essential oils (EOs)	262:287	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	4	43	dep	oil-containing	591:604	arg1	cellulose-based					607:621	cellulose-based	607:621	cellulose-based	607:621	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	1	44	with	saturated	225:233	arg1	eucalyptus					247:256	eucalyptus	247:256	eucalyptus	247:256	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	44	with	saturated	225:233	arg1	thyme					240:244	thyme	240:244	thyme	240:244	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	44	with	saturated	225:233	arg1	oils					278:281	clove essential oils	262:281	clove essential oils (EOs)	262:287	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	44	with	saturated	225:233	arg1	EOs					284:286	EOs	284:286	EOs	284:286	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	45	theme	biopolymers	188:198	arg1	one					162:164	one	162:164	one	162:164	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	45	theme	biopolymers	188:198	arg1	cellulose					146:154	bacterial cellulose	136:154	bacterial cellulose (BC)	136:159	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	45	theme	biopolymers	188:198	arg1	biopolymers					188:198	the most promising biopolymers	169:198	the most promising biopolymers of the recent years	169:218	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	46	theme	essential	268:276	arg1	EOs					284:286	EOs	284:286	EOs	284:286	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	46	theme	essential	268:276	arg1	oils					278:281	clove essential oils	262:281	clove essential oils (EOs)	262:287	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	8	47	theme	dressings	1313:1321	arg1	suitability					1282:1292	the suitability	1278:1292	the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells	1278:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	9	48	theme	thyme	1523:1527	arg1	effects					1512:1518	cytotoxic effects	1502:1518	cytotoxic effects of thyme and clove EOs towards cell lines in vitro	1502:1569	However, due to cytotoxic effects of thyme and clove EOs towards cell lines in vitro, the eucalyptus EO-saturated BC dressing is of highest potential to be further applied.
30718663	5	49	theme	thyme	963:967	arg1	EO					969:970	the thyme EO	959:970	the thyme EO	959:970	The results revealed the high ability of BC dressings to absorb and subsequently release EOs from within their microstructure; the highest number of compounds able to adhere to HA was found in the thyme EO.
30718663	8	50	theme	BC	1310:1311	arg1	dressings					1313:1321	EO-saturated BC dressings	1297:1321	EO-saturated BC dressings	1297:1321	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	7	51	theme	pseudomonal	1211:1221	arg1	biofilm					1223:1229	pseudomonal biofilm	1211:1229	pseudomonal biofilm	1211:1229	The clove EO displayed the highest eradication ability toward staphylococcal, while the thyme EO against pseudomonal biofilm.
30718663	0	52	theme	Essential	51:59	arg1	Oils					61:64	Essential Oils	51:64	Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite	51:115	Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite.
30718663	8	53	theme	reactive	1418:1425	arg1	species					1434:1440	reactive oxygen species	1418:1440	reactive oxygen species production by immune system effector cells	1418:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	8	54	theme	staphylococcal	1352:1365	arg1	biofilm					1367:1373	pseudomonal and staphylococcal biofilm	1336:1373	pseudomonal and staphylococcal biofilm	1336:1373	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	7	55	theme	highest	1133:1139	arg1	ability					1153:1159	the highest eradication ability	1129:1159	the highest eradication ability	1129:1159	The clove EO displayed the highest eradication ability toward staphylococcal, while the thyme EO against pseudomonal biofilm.
30718663	8	56	theme	HA	1378:1379	arg1	surface					1381:1387	HA surface	1378:1387	HA surface	1378:1387	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	8	57	theme	pseudomonal	1336:1346	arg1	biofilm					1367:1373	pseudomonal and staphylococcal biofilm	1336:1373	pseudomonal and staphylococcal biofilm	1336:1373	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	6	58	theme	high	1042:1045	arg1	cytotoxicity					1047:1058	high cytotoxicity	1042:1058	high cytotoxicity towards fibroblast and osteoblast cell lines	1042:1103	The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines.
30718663	4	59	from	impact	686:691	arg1	production					726:735	reactive oxygen species (ROS) production	696:735	reactive oxygen species (ROS) production by macrophages	696:750	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	8	60	theme	species	1434:1440	arg1	production					1442:1451	reactive oxygen species production	1418:1451	reactive oxygen species production by immune system effector cells	1418:1483	Taken together, the results obtained indicate the suitability of EO-saturated BC dressings to eradicate pseudomonal and staphylococcal biofilm on HA surface and moreover, to not trigger reactive oxygen species production by immune system effector cells.
30718663	6	61	theme	clove	1022:1026	arg1	EOs					1028:1030	clove EOs	1022:1030	clove EOs	1022:1030	The eucalyptus EO displayed low, while thyme and clove EOs displayed high cytotoxicity towards fibroblast and osteoblast cell lines.
30718663	4	62	theme	oil-containing	591:604	arg1	dressings					623:631	oil-containing, cellulose-based dressings	591:631	oil-containing, cellulose-based dressings	591:631	Additionally, cytotoxicity of oil-containing, cellulose-based dressings towards osteoblasts and fibroblasts as well as their impact on reactive oxygen species (ROS) production by macrophages was assessed.
30718663	1	63	theme	bacterial	136:144	arg1	one					162:164	one	162:164	one	162:164	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	63	theme	bacterial	136:144	arg1	cellulose					146:154	bacterial cellulose	136:154	bacterial cellulose (BC)	136:159	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	63	theme	bacterial	136:144	arg1	biopolymers					188:198	the most promising biopolymers	169:198	the most promising biopolymers of the recent years	169:218	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	63	theme	bacterial	136:144	arg1	BC					157:158	BC	157:158	BC	157:158	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	1	64	theme	recent	207:212	arg1	years					214:218	the recent years	203:218	the recent years	203:218	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30718663	0	65	theme	Biocellulose	13:24	arg1	Carrier					26:32	Biocellulose Carrier	13:32	Biocellulose Carrier	13:32	Potential of Biocellulose Carrier Impregnated with Essential Oils to Fight Against Biofilms Formed on Hydroxyapatite.
30718663	3	66	theme	exact	474:478	arg1	composition					480:490	the exact composition	470:490	the exact composition	470:490	Moreover, the exact composition and ability of particular EO molecules to adhere to HA was assessed.
30718663	1	67	theme	years	214:218	arg1	biopolymers					188:198	the most promising biopolymers	169:198	the most promising biopolymers of the recent years	169:218	In this research, bacterial cellulose (BC), one of the most promising biopolymers of the recent years, was saturated with thyme, eucalyptus and clove essential oils (EOs) and applied against staphylococcal and pseudomonal biofilms formed on hydroxyapatite (HA).
30256506	10	0	with	rats	1582:1585	arg1	CPI					1592:1594	CPI	1592:1594	CPI	1592:1594	Conversely, levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae were decreased in rats with CPI.
30256506	0	1	from	extract	169:175	arg1	diet					184:187	the diet	180:187	the diet	180:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	14	2	theme	acid	2137:2140	arg1	levels					2102:2107	the levels	2098:2107	the levels of butyric acid and caproic acid	2098:2140	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	3	3	with	Rats	496:499	arg1	pellets					514:520	placebo pellets	506:520	placebo pellets	506:520	Rats with placebo pellets served as healthy controls.
30256506	13	4	theme	GGM	1881:1883	arg1	extract					1885:1891	the GGM extract	1877:1891	the GGM extract	1877:1891	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	3	5	theme	healthy	532:538	arg1	Rats					496:499	Rats	496:499	Rats with placebo pellets	496:520	Rats with placebo pellets served as healthy controls.
30256506	3	5	theme	healthy	532:538	arg1	controls					540:547	healthy controls	532:547	healthy controls	532:547	Rats with placebo pellets served as healthy controls.
30256506	9	6	theme	significant	1332:1342	arg1	increase					1344:1351	a significant increase	1330:1351	a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1330:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	14	7	theme	butyric	2112:2118	arg1	acid					2120:2123	butyric acid	2112:2123	butyric acid	2112:2123	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	11	8	theme	SCFA	1597:1600	arg1	concentrations					1638:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	2	9	with	strain	403:408	arg1	pellets					474:480	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	pellets	474:480	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	2	9	with	strain	403:408	arg1	testosterone					428:439	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	testosterone	428:439	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	10	10	theme	Lactobacillus	1530:1542	arg1	levels					1497:1502	levels	1497:1502	levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae	1497:1562	Conversely, levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae were decreased in rats with CPI.
30256506	9	11	theme	microbial	1360:1368	arg1	Peptococcaceae					1442:1455	Peptococcaceae	1442:1455	Peptococcaceae	1442:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	11	theme	microbial	1360:1368	arg1	Allobaculum					1426:1436	Allobaculum	1426:1436	Allobaculum	1426:1436	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	11	theme	microbial	1360:1368	arg1	populations					1370:1380	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	11	theme	microbial	1360:1368	arg1	Odoribacter					1397:1407	Odoribacter	1397:1407	Odoribacter	1397:1407	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	10	12	theme	uniformis	1519:1527	arg1	levels					1497:1502	levels	1497:1502	levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae	1497:1562	Conversely, levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae were decreased in rats with CPI.
30256506	15	13	theme	secondary	2294:2302	arg1	changes					2304:2310	secondary changes	2294:2310	secondary changes in SCFAs and LPS due to gut microbiota alteration	2294:2360	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	6	14	theme	acid	1001:1004	arg1	concentrations					1013:1026	short-chain fatty acid (SCFA) concentrations	983:1026	short-chain fatty acid (SCFA) concentrations using gas chromatography	983:1051	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	15	15	from	CPI	2224:2226	arg1	rats					2231:2234	rats	2231:2234	rats	2231:2234	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	4	16	theme	tap	622:624	arg1	water					626:630	plain tap water	616:630	plain tap water (control group)	616:646	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	16	theme	tap	622:624	arg1	group					641:645	control group	633:645	control group	633:645	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	11	17	with	rats	1702:1705	arg1	CPI					1712:1714	CPI	1712:1714	CPI	1712:1714	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	1	18	theme	dietary	216:222	arg1	effects					224:230	dietary effects	216:230	dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI)	216:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	15	19	theme	CONCLUSIONS	2183:2193	arg1	CPI					2224:2226	CONCLUSIONS Hormone-induced nonbacterial CPI	2183:2226	CONCLUSIONS Hormone-induced nonbacterial CPI in rats	2183:2234	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	10	20	theme	Lachnospiraceae	1548:1562	arg1	levels					1497:1502	levels	1497:1502	levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae	1497:1562	Conversely, levels of Bacteroides uniformis, Lactobacillus and Lachnospiraceae were decreased in rats with CPI.
30256506	13	21	theme	CPI	2023:2025	arg1	rats					2027:2030	CPI rats	2023:2030	CPI rats drinking tap water only	2023:2054	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	5	22	theme	total	841:845	arg1	DNA					847:849	total DNA	841:849	total DNA	841:849	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	4	23	theme	%	680:680	arg1	extract					720:726	2% galactoglucomannan-rich hemicellulose extract	679:726	2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce	679:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	23	theme	%	680:680	arg1	group					733:737	GGM group	729:737	GGM group	729:737	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	0	24	theme	hemicellulose	155:167	arg1	extract					169:175	a galactoglucomannan-rich hemicellulose extract	129:175	a galactoglucomannan-rich hemicellulose extract in the diet	129:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	15	25	theme	specific	2255:2262	arg1	changes					2264:2270	specific changes	2255:2270	specific changes in gut microbiota	2255:2288	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	11	26	theme	faecal	1680:1685	arg1	samples					1687:1693	the faecal samples	1676:1693	the faecal samples of the rats with CPI	1676:1714	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	15	27	theme	gut	2336:2338	arg1	alteration					2351:2360	gut microbiota alteration	2336:2360	gut microbiota alteration	2336:2360	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	4	28	from	spruce	752:757	arg1	extract					720:726	2% galactoglucomannan-rich hemicellulose extract	679:726	2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce	679:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	28	from	spruce	752:757	arg1	group					733:737	GGM group	729:737	GGM group	729:737	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	15	29	theme	nonbacterial	2211:2222	arg1	CPI					2224:2226	CONCLUSIONS Hormone-induced nonbacterial CPI	2183:2226	CONCLUSIONS Hormone-induced nonbacterial CPI in rats	2183:2234	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	15	30	theme	due	2329:2331	arg1	SCFAs					2315:2319	SCFAs	2315:2319	SCFAs	2315:2319	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	5	31	theme	study	834:838	arg1	end					823:825	the end	819:825	the end of the study	819:838	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	11	32	theme	rats	1702:1705	arg1	samples					1687:1693	the faecal samples	1676:1693	the faecal samples of the rats with CPI	1676:1714	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	4	33	with	Rats	550:553	arg1	CPI					560:562	CPI	560:562	CPI	560:562	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	8	34	theme	treatment	1280:1288	arg1	groups					1290:1295	the treatment groups	1276:1295	the treatment groups	1276:1295	RESULTS The microbial biodiversity was significantly different between the treatment groups.
30256506	7	35	theme	serum	1111:1115	arg1	samples					1117:1123	serum samples	1111:1123	serum samples	1111:1123	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	6	36	theme	short-chain	983:993	arg1	SCFA					1007:1010	SCFA	1007:1010	SCFA	1007:1010	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	6	36	theme	short-chain	983:993	arg1	acid					1001:1004	short-chain fatty acid	983:1004	short-chain fatty acid (SCFA) concentrations using gas chromatography	983:1051	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	2	37	theme	17β-oestradiol	445:458	arg1	pellets					474:480	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	pellets	474:480	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	13	38	with	rats	1847:1850	arg1	CPI					1857:1859	CPI	1857:1859	CPI	1857:1859	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	4	39	theme	hemicellulose	706:718	arg1	extract					720:726	2% galactoglucomannan-rich hemicellulose extract	679:726	2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce	679:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	39	theme	hemicellulose	706:718	arg1	group					733:737	GGM group	729:737	GGM group	729:737	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	0	40	theme	Chronic	0:6	arg1	inflammation					30:41	Chronic nonbacterial prostate inflammation	0:41	Chronic nonbacterial prostate inflammation in a rat model	0:56	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	1	41	theme	rat	271:273	arg1	model					275:279	a rat model	269:279	a rat model of nonbacterial chronic prostate inflammation (CPI)	269:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	16	42	contain	have	2422:2425	arg1	compounds					2408:2416	fermentable compounds	2396:2416	fermentable compounds	2396:2416	Our results further suggest that fermentable compounds may have a beneficial effect on CPI.
30256506	16	42	contain	have	2422:2425	arg2	effect					2440:2445	a beneficial effect	2427:2445	a beneficial effect	2427:2445	Our results further suggest that fermentable compounds may have a beneficial effect on CPI.
30256506	0	43	theme	prostate	21:28	arg1	inflammation					30:41	Chronic nonbacterial prostate inflammation	0:41	Chronic nonbacterial prostate inflammation in a rat model	0:56	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	2	44	theme	hormone	466:472	arg1	pellets					474:480	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	pellets	474:480	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	13	45	with	treatment	1862:1870	arg1	extract					1885:1891	the GGM extract	1877:1891	the GGM extract	1877:1891	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	2	46	theme	rat	399:401	arg1	strain					403:408	the Wistar rat strain	388:408	the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks	388:493	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	1	47	theme	chronic	297:303	arg1	CPI					328:330	CPI	328:330	CPI	328:330	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	1	47	theme	chronic	297:303	arg1	inflammation					314:325	nonbacterial chronic prostate inflammation	284:325	nonbacterial chronic prostate inflammation (CPI)	284:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	2	48	theme	E2	461:462	arg1	pellets					474:480	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	pellets	474:480	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	13	49	dep	B.	1989:1990	arg1	uniformis					1992:2000	uniformis	1992:2000	uniformis	1992:2000	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	11	50	theme	caproic-acid	1625:1636	arg1	concentrations					1638:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	13	51	theme	Clostridiaceae	1948:1961	arg1	abundance					1919:1927	the abundance	1915:1927	the abundance of Odoribacter and Clostridiaceae levels	1915:1968	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	2	52	theme	MATERIALS	334:342	arg1	CPI					369:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	0	53	theme	rat	48:50	arg1	model					52:56	a rat model	46:56	a rat model	46:56	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	1	54	theme	inflammation	314:325	arg1	model					275:279	a rat model	269:279	a rat model of nonbacterial chronic prostate inflammation (CPI)	269:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	12	55	theme	serum	1761:1765	arg1	LBP					1767:1769	serum LBP	1761:1769	serum LBP	1761:1769	In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones.
30256506	6	56	theme	gas	1034:1036	arg1	chromatography					1038:1051	gas chromatography	1034:1051	gas chromatography	1034:1051	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	8	57	dep	RESULTS	1205:1211	arg1	biodiversity					1227:1238	The microbial biodiversity	1213:1238	RESULTS The microbial biodiversity	1205:1238	RESULTS The microbial biodiversity was significantly different between the treatment groups.
30256506	2	58	theme	Wistar	392:397	arg1	strain					403:408	the Wistar rat strain	388:408	the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks	388:493	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	9	59	dep	populations	1370:1380	arg1	Peptococcaceae					1442:1455	Peptococcaceae	1442:1455	Peptococcaceae	1442:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	59	dep	populations	1370:1380	arg1	Allobaculum					1426:1436	Allobaculum	1426:1436	Allobaculum	1426:1436	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	59	dep	populations	1370:1380	arg1	populations					1370:1380	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	59	dep	populations	1370:1380	arg1	Odoribacter					1397:1407	Odoribacter	1397:1407	Odoribacter	1397:1407	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	5	60	theme	gene	921:924	arg1	sequencing					926:935	rRNA gene sequencing	916:935	rRNA gene sequencing	916:935	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	9	61	from	increase	1344:1351	arg1	Peptococcaceae					1442:1455	Peptococcaceae	1442:1455	Peptococcaceae	1442:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	61	from	increase	1344:1351	arg1	Allobaculum					1426:1436	Allobaculum	1426:1436	Allobaculum	1426:1436	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	61	from	increase	1344:1351	arg1	populations					1370:1380	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	61	from	increase	1344:1351	arg1	Odoribacter					1397:1407	Odoribacter	1397:1407	Odoribacter	1397:1407	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	13	62	theme	Odoribacter	1932:1942	arg1	abundance					1919:1927	the abundance	1915:1927	the abundance of Odoribacter and Clostridiaceae levels	1915:1968	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	2	63	theme	METHODS	348:354	arg1	CPI					369:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	1	64	theme	microbiota	243:252	arg1	composition					254:264	the gut microbiota composition	235:264	the gut microbiota composition	235:264	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	7	65	theme	bacterial	1155:1163	arg1	load					1190:1193	bacterial lipopolysaccharide (LPS) load	1155:1193	bacterial lipopolysaccharide (LPS) load in blood	1155:1202	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	14	66	theme	caproic	2129:2135	arg1	acid					2137:2140	caproic acid	2129:2140	caproic acid	2129:2140	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	15	67	theme	Hormone-induced	2195:2209	arg1	CPI					2224:2226	CONCLUSIONS Hormone-induced nonbacterial CPI	2183:2226	CONCLUSIONS Hormone-induced nonbacterial CPI in rats	2183:2234	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	14	68	theme	acid	2120:2123	arg1	levels					2102:2107	the levels	2098:2107	the levels of butyric acid and caproic acid	2098:2140	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	11	69	theme	valeric-	1612:1619	arg1	concentrations					1638:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	7	70	theme	Lipopolysaccharide-binding	1054:1079	arg1	protein					1081:1087	Lipopolysaccharide-binding protein	1054:1087	Lipopolysaccharide-binding protein (LBP)	1054:1093	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	7	70	theme	Lipopolysaccharide-binding	1054:1079	arg1	LBP					1090:1092	LBP	1090:1092	LBP	1090:1092	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	7	70	theme	Lipopolysaccharide-binding	1054:1079	arg1	indicator					1141:1149	an indirect indicator	1129:1149	an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood	1129:1202	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	16	71	theme	fermentable	2396:2406	arg1	compounds					2408:2416	fermentable compounds	2396:2416	fermentable compounds	2396:2416	Our results further suggest that fermentable compounds may have a beneficial effect on CPI.
30256506	11	72	theme	butyric-	1602:1609	arg1	concentrations					1638:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations	1597:1651	SCFA butyric-, valeric- and caproic-acid concentrations were also decreased in the faecal samples of the rats with CPI.
30256506	9	73	theme	gut	1356:1358	arg1	Peptococcaceae					1442:1455	Peptococcaceae	1442:1455	Peptococcaceae	1442:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	73	theme	gut	1356:1358	arg1	Allobaculum					1426:1436	Allobaculum	1426:1436	Allobaculum	1426:1436	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	73	theme	gut	1356:1358	arg1	populations					1370:1380	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae	1356:1455	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	9	73	theme	gut	1356:1358	arg1	Odoribacter					1397:1407	Odoribacter	1397:1407	Odoribacter	1397:1407	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	7	74	from	load	1190:1193	arg1	blood					1198:1202	blood	1198:1202	blood	1198:1202	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	4	75	theme	control	633:639	arg1	water					626:630	plain tap water	616:630	plain tap water (control group)	616:646	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	75	theme	control	633:639	arg1	group					641:645	control group	633:645	control group	633:645	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	5	76	theme	Faecal	786:791	arg1	samples					793:799	Faecal samples	786:799	Faecal samples	786:799	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	4	77	theme	plain	616:620	arg1	water					626:630	plain tap water	616:630	plain tap water (control group)	616:646	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	77	theme	plain	616:620	arg1	group					641:645	control group	633:645	control group	633:645	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	0	78	theme	galactoglucomannan-rich	131:153	arg1	extract					169:175	a galactoglucomannan-rich hemicellulose extract	129:175	a galactoglucomannan-rich hemicellulose extract in the diet	129:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	0	79	mod	modified	115:122	arg3	extract					169:175	a galactoglucomannan-rich hemicellulose extract	129:175	a galactoglucomannan-rich hemicellulose extract in the diet	129:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	0	79	mod	modified	115:122	arg1	changes					77:83	changes	77:83	changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet	77:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	1	80	from	effects	224:230	arg1	composition					254:264	the gut microbiota composition	235:264	the gut microbiota composition	235:264	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	1	80	from	effects	224:230	arg1	model					275:279	a rat model	269:279	a rat model of nonbacterial chronic prostate inflammation (CPI)	269:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	15	81	theme	gut	2275:2277	arg1	microbiota					2279:2288	gut microbiota	2275:2288	gut microbiota	2275:2288	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	9	82	theme	healthy	1471:1477	arg1	rats					1479:1482	healthy rats	1471:1482	healthy rats	1471:1482	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	4	83	theme	2	679:679	arg1	%					680:680	%	680:680	%	680:680	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	7	84	theme	indirect	1132:1139	arg1	protein					1081:1087	Lipopolysaccharide-binding protein	1054:1087	Lipopolysaccharide-binding protein (LBP)	1054:1093	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	7	84	theme	indirect	1132:1139	arg1	indicator					1141:1149	an indirect indicator	1129:1149	an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood	1129:1202	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	13	85	theme	tap	2041:2043	arg1	water					2045:2049	tap water	2041:2049	tap water only	2041:2054	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	15	86	from	changes	2264:2270	arg1	LPS					2325:2327	LPS	2325:2327	LPS	2325:2327	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	15	86	from	changes	2264:2270	arg1	SCFAs					2315:2319	SCFAs	2315:2319	SCFAs	2315:2319	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	15	86	from	changes	2264:2270	arg1	microbiota					2279:2288	gut microbiota	2275:2288	gut microbiota	2275:2288	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	0	87	from	inflammation	30:41	arg1	model					52:56	a rat model	46:56	a rat model	46:56	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	8	88	theme	microbial	1217:1225	arg1	biodiversity					1227:1238	The microbial biodiversity	1213:1238	RESULTS The microbial biodiversity	1205:1238	RESULTS The microbial biodiversity was significantly different between the treatment groups.
30256506	15	89	theme	microbiota	2340:2349	arg1	alteration					2351:2360	gut microbiota alteration	2336:2360	gut microbiota alteration	2336:2360	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	4	90	theme	tap	651:653	arg1	water					655:659	tap water	651:659	tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies)	651:771	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	90	theme	tap	651:653	arg1	abies					766:770	Picea abies	760:770	Picea abies	760:770	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	0	91	theme	gut	88:90	arg1	microbiota					92:101	gut microbiota	88:101	gut microbiota	88:101	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	5	92	theme	bacterial	874:882	arg1	composition					884:894	the bacterial composition	870:894	the bacterial composition	870:894	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	4	93	theme	GGM	729:731	arg1	extract					720:726	2% galactoglucomannan-rich hemicellulose extract	679:726	2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce	679:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	93	theme	GGM	729:731	arg1	group					733:737	GGM group	729:737	GGM group	729:737	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	12	94	theme	healthy	1825:1831	arg1	ones					1833:1836	healthy ones	1825:1836	healthy ones	1825:1836	In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones.
30256506	6	95	theme	fatty	995:999	arg1	SCFA					1007:1010	SCFA	1007:1010	SCFA	1007:1010	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	6	95	theme	fatty	995:999	arg1	acid					1001:1004	short-chain fatty acid	983:1004	short-chain fatty acid (SCFA) concentrations using gas chromatography	983:1051	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	15	96	from	changes	2304:2310	arg1	LPS					2325:2327	LPS	2325:2327	LPS	2325:2327	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	15	96	from	changes	2304:2310	arg1	SCFAs					2315:2319	SCFAs	2315:2319	SCFAs	2315:2319	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	15	96	from	changes	2304:2310	arg1	microbiota					2279:2288	gut microbiota	2275:2288	gut microbiota	2275:2288	CONCLUSIONS Hormone-induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFAs and LPS due to gut microbiota alteration.
30256506	0	97	theme	nonbacterial	8:19	arg1	inflammation					30:41	Chronic nonbacterial prostate inflammation	0:41	Chronic nonbacterial prostate inflammation in a rat model	0:56	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	13	98	theme	B.	1989:1990	arg1	levels					2002:2007	the B. uniformis levels	1985:2007	the B. uniformis levels	1985:2007	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	4	99	theme	galactoglucomannan-rich	682:704	arg1	extract					720:726	2% galactoglucomannan-rich hemicellulose extract	679:726	2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce	679:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	99	theme	galactoglucomannan-rich	682:704	arg1	group					733:737	GGM group	729:737	GGM group	729:737	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	14	100	theme	LBP	2169:2171	arg1	levels					2159:2164	the levels	2155:2164	the levels of LBP in serum	2155:2180	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	4	101	theme	Picea	760:764	arg1	water					655:659	tap water	651:659	tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies)	651:771	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	4	101	theme	Picea	760:764	arg1	abies					766:770	Picea abies	760:770	Picea abies	760:770	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	12	102	theme	CPI	1802:1804	arg1	rats					1806:1809	CPI rats	1802:1809	CPI rats	1802:1809	In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones.
30256506	13	103	dep	Odoribacter	1932:1942	arg1	levels					1963:1968	levels	1963:1968	levels	1963:1968	Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only.
30256506	14	104	from	levels	2159:2164	arg1	serum					2176:2180	serum	2176:2180	serum	2176:2180	In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum.
30256506	2	105	theme	subcutaneous	415:426	arg1	testosterone					428:439	subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets	415:480	testosterone	428:439	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	1	106	theme	nonbacterial	284:295	arg1	CPI					328:330	CPI	328:330	CPI	328:330	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	1	106	theme	nonbacterial	284:295	arg1	inflammation					314:325	nonbacterial chronic prostate inflammation	284:325	nonbacterial chronic prostate inflammation (CPI)	284:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	6	107	theme	faecal	951:956	arg1	samples					958:964	faecal samples	951:964	faecal samples	951:964	In addition, faecal samples were assayed for short-chain fatty acid (SCFA) concentrations using gas chromatography.
30256506	0	108	theme	microbiota	92:101	arg1	changes					77:83	changes	77:83	changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet	77:187	Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan-rich hemicellulose extract in the diet.
30256506	1	109	theme	prostate	305:312	arg1	CPI					328:330	CPI	328:330	CPI	328:330	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	1	109	theme	prostate	305:312	arg1	inflammation					314:325	nonbacterial chronic prostate inflammation	284:325	nonbacterial chronic prostate inflammation (CPI)	284:331	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
30256506	12	110	theme	acid	1737:1740	arg1	concentrations					1742:1755	acetic acid concentrations	1730:1755	acetic acid concentrations	1730:1755	In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones.
30256506	16	111	theme	beneficial	2429:2438	arg1	effect					2440:2445	a beneficial effect	2427:2445	a beneficial effect	2427:2445	Our results further suggest that fermentable compounds may have a beneficial effect on CPI.
30256506	12	112	theme	acetic	1730:1735	arg1	acid					1737:1740	acetic acid	1730:1740	acetic acid concentrations	1730:1755	In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones.
30256506	4	113	theme	Norway	745:750	arg1	spruce					752:757	Norway spruce	745:757	Norway spruce	745:757	Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan-rich hemicellulose extract (GGM group) from Norway spruce (Picea abies) for 5 weeks.
30256506	3	114	theme	placebo	506:512	arg1	pellets					514:520	placebo pellets	506:520	placebo pellets	506:520	Rats with placebo pellets served as healthy controls.
30256506	5	115	theme	rRNA	916:919	arg1	sequencing					926:935	rRNA gene sequencing	916:935	rRNA gene sequencing	916:935	Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing.
30256506	7	116	theme	lipopolysaccharide	1165:1182	arg1	load					1190:1193	bacterial lipopolysaccharide (LPS) load	1155:1193	bacterial lipopolysaccharide (LPS) load in blood	1155:1202	Lipopolysaccharide-binding protein (LBP) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood.
30256506	9	117	with	rats	1305:1308	arg1	CPI					1315:1317	CPI	1315:1317	CPI	1315:1317	In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae, Odoribacter, Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats.
30256506	2	118	theme	Nonbacterial	356:367	arg1	CPI					369:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI	334:371	MATERIALS AND METHODS Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β-oestradiol (E2 ) hormone pellets for 18 weeks.
30256506	1	119	theme	gut	239:241	arg1	composition					254:264	the gut microbiota composition	235:264	the gut microbiota composition	235:264	OBJECTIVES To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI).
31465803	0	0	from	character	13:21	arg1	officinalis					66:76	processed Morindae officinalis	47:76	processed Morindae officinalis	47:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	0	0	from	character	13:21	arg1	damage					110:115	anti-liver damage	99:115	anti-liver damage	99:115	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	4	1	theme	MOP-100	664:670	arg1	weight					654:659	The molecular weight	640:659	The molecular weight of MOP-100	640:670	The molecular weight of MOP-100 was a wide distribution.
31465803	4	1	theme	MOP-100	664:670	arg1	distribution					683:694	a wide distribution	676:694	a wide distribution	676:694	The molecular weight of MOP-100 was a wide distribution.
31465803	6	2	theme	molecular	898:906	arg1	weight					908:913	molecular weight	898:913	molecular weight of 2071 Da	898:924	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	2	3	from	effect	326:331	arg1	root					387:390	the processed root	373:390	the processed root of Morinda officinalis	373:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	4	theme	polysaccharides	342:356	arg1	composition					293:303	the chemical composition	280:303	the chemical composition	280:303	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	4	theme	polysaccharides	342:356	arg1	effect					326:331	liver-protective effect	309:331	liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro	309:482	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	11	5	theme	disease	1544:1550	arg1	treatment					1509:1517	treatment	1509:1517	treatment of immune-mediated liver disease in the future	1509:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	0	6	theme	anti-liver	99:108	arg1	damage					110:115	anti-liver damage	99:115	anti-liver damage	99:115	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	3	7	theme	herbal	511:516	arg1	medicine					518:525	the herbal medicine	507:525	the herbal medicine	507:525	The processed root of the herbal medicine was extracted with water at 100 °C, then precipitated with ethanol, dialyzed and freeze-dried to obtain MOP-100.
31465803	2	8	from	composition	293:303	arg1	root					387:390	the processed root	373:390	the processed root of Morinda officinalis	373:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	7	9	theme	macrophages	995:1005	arg1	infiltration					963:974	the infiltration	959:974	the infiltration of neutrophils and macrophages into liver	959:1016	MOP-100 significantly inhibited the infiltration of neutrophils and macrophages into liver, improved the hepatic injury induced by ConA in mice.
31465803	9	10	theme	hepatocytes	1244:1254	arg1	secretion					1222:1230	the HBeAg secretion	1212:1230	the HBeAg secretion of Hep2.5.5 hepatocytes	1212:1254	MOP-100 also significantly inhibited the HBeAg secretion of Hep2.5.5 hepatocytes in vitro.
31465803	10	11	theme	ConA-induced	1351:1362	arg1	damage					1364:1369	ConA-induced damage	1351:1369	ConA-induced damage in vitro	1351:1378	MOP-60 and MOP-60-100 both exhibited good activity of protecting hepatocytes against ConA-induced damage in vitro.
31465803	10	12	dep	MOP-60	1266:1271	arg1	both					1288:1291	both	1288:1291	both	1288:1291	MOP-60 and MOP-60-100 both exhibited good activity of protecting hepatocytes against ConA-induced damage in vitro.
31465803	3	13	theme	medicine	518:525	arg1	root					499:502	The processed root	485:502	The processed root of the herbal medicine	485:525	The processed root of the herbal medicine was extracted with water at 100 °C, then precipitated with ethanol, dialyzed and freeze-dried to obtain MOP-100.
31465803	2	14	theme	liver-protective	309:324	arg1	effect					326:331	liver-protective effect	309:331	liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro	309:482	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	11	15	theme	hepatoprotective	1459:1474	arg1	effect					1476:1481	significant hepatoprotective effect	1447:1481	significant hepatoprotective effect	1447:1481	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	6	16	theme	inulin-type	859:869	arg1	fructooligosaccharide					871:891	an inulin-type fructooligosaccharide	856:891	an inulin-type fructooligosaccharide with molecular weight of 2071 Da	856:924	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	11	17	from	disease	1544:1550	arg1	future					1559:1564	future	1559:1564	future	1559:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	8	18	theme	cells	1123:1127	arg1	proliferation					1090:1102	the proliferation	1086:1102	the proliferation of human liver LO2 cells	1086:1127	It stimulated the proliferation of human liver LO2 cells and decreased the cell death induced by ConA.
31465803	11	19	used	used	1500:1503	arg2	it					1488:1489	it	1488:1489	it	1488:1489	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	2	20	theme	study	254:258	arg1	aims					234:237	The aims	230:237	The aims of the present study	230:258	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	21	theme	processed	377:385	arg1	root					387:390	the processed root	373:390	the processed root of Morinda officinalis	373:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	0	22	theme	chemical	4:11	arg1	character					13:21	The chemical character	0:21	The chemical character of polysaccharides from processed Morindae officinalis	0:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	5	23	theme	processed	710:718	arg1	root					720:723	the processed root	706:723	the processed root	706:723	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	11	24	from	treatment	1509:1517	arg1	future					1559:1564	future	1559:1564	future	1559:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	10	25	from	damage	1364:1369	arg1	vitro					1374:1378	vitro	1374:1378	vitro	1374:1378	MOP-60 and MOP-60-100 both exhibited good activity of protecting hepatocytes against ConA-induced damage in vitro.
31465803	4	26	theme	wide	678:681	arg1	weight					654:659	The molecular weight	640:659	The molecular weight of MOP-100	640:670	The molecular weight of MOP-100 was a wide distribution.
31465803	4	26	theme	wide	678:681	arg1	distribution					683:694	a wide distribution	676:694	a wide distribution	676:694	The molecular weight of MOP-100 was a wide distribution.
31465803	9	27	theme	HBeAg	1216:1220	arg1	secretion					1222:1230	the HBeAg secretion	1212:1230	the HBeAg secretion of Hep2.5.5 hepatocytes	1212:1254	MOP-100 also significantly inhibited the HBeAg secretion of Hep2.5.5 hepatocytes in vitro.
31465803	2	28	theme	chemical	284:291	arg1	composition					293:303	the chemical composition	280:303	the chemical composition	280:303	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	11	29	theme	immune-mediated	1522:1536	arg1	disease					1544:1550	immune-mediated liver disease	1522:1550	immune-mediated liver disease in the future	1522:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	5	30	theme	crude	787:791	arg1	MOP-60					809:814	MOP-60	809:814	MOP-60	809:814	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	5	30	theme	crude	787:791	arg1	polysaccharides					793:807	two crude polysaccharides MOP-60 and MOP-60-100	783:829	two crude polysaccharides MOP-60 and MOP-60-100	783:829	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	5	30	theme	crude	787:791	arg1	MOP-60-100					820:829	MOP-60-100	820:829	MOP-60-100	820:829	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	2	31	theme	Concanavalin	423:434	arg1	ConA					439:442	ConA	439:442	ConA	439:442	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	31	theme	Concanavalin	423:434	arg1	A					436:436	Concanavalin A	423:436	Concanavalin A (ConA)	423:443	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	11	32	theme	Morinda	1405:1411	arg1	polysaccharides					1425:1439	Morinda officinalis polysaccharides	1405:1439	Morinda officinalis polysaccharides	1405:1439	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	11	33	dep	suggested	1395:1403	arg1	exert					1441:1445	exert	1441:1445	suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect	1395:1481	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	0	34	from	effects	88:94	arg1	officinalis					66:76	processed Morindae officinalis	47:76	processed Morindae officinalis	47:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	0	34	from	effects	88:94	arg1	damage					110:115	anti-liver damage	99:115	anti-liver damage	99:115	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	2	35	theme	officinalis	403:413	arg1	root					387:390	the processed root	373:390	the processed root of Morinda officinalis	373:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	1	36	theme	traditional	198:208	arg1	medicines					210:218	traditional medicines	198:218	traditional medicines in China	198:227	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	10	37	theme	good	1303:1306	arg1	activity					1308:1315	good activity	1303:1315	good activity of protecting hepatocytes against ConA-induced damage in vitro	1303:1378	MOP-60 and MOP-60-100 both exhibited good activity of protecting hepatocytes against ConA-induced damage in vitro.
31465803	2	38	theme	Morinda	395:401	arg1	officinalis					403:413	Morinda officinalis	395:413	Morinda officinalis	395:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	39	theme	liver	453:457	arg1	damage					459:464	Concanavalin A (ConA)-induced liver damage	423:464	Concanavalin A (ConA)-induced liver damage	423:464	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	0	40	theme	polysaccharides	26:40	arg1	character					13:21	The chemical character	0:21	The chemical character of polysaccharides from processed Morindae officinalis	0:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	0	40	theme	polysaccharides	26:40	arg1	effects					88:94	their effects	82:94	their effects on anti-liver damage	82:115	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	9	41	theme	Hep2.5.5	1235:1242	arg1	hepatocytes					1244:1254	Hep2.5.5 hepatocytes	1235:1254	Hep2.5.5 hepatocytes	1235:1254	MOP-100 also significantly inhibited the HBeAg secretion of Hep2.5.5 hepatocytes in vitro.
31465803	0	42	theme	Morindae	57:64	arg1	officinalis					66:76	processed Morindae officinalis	47:76	processed Morindae officinalis	47:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	11	43	theme	significant	1447:1457	arg1	effect					1476:1481	significant hepatoprotective effect	1447:1481	significant hepatoprotective effect	1447:1481	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	8	44	theme	human	1107:1111	arg1	cells					1123:1127	human liver LO2 cells	1107:1127	human liver LO2 cells	1107:1127	It stimulated the proliferation of human liver LO2 cells and decreased the cell death induced by ConA.
31465803	0	45	from	officinalis	66:76	arg1	character					13:21	The chemical character	0:21	The chemical character of polysaccharides from processed Morindae officinalis	0:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	0	45	from	officinalis	66:76	arg1	effects					88:94	their effects	82:94	their effects on anti-liver damage	82:115	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	0	45	from	officinalis	66:76	arg1	polysaccharides					26:40	polysaccharides	26:40	polysaccharides from processed Morindae officinalis	26:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	1	46	from	medicines	210:218	arg1	China					223:227	China	223:227	China	223:227	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	2	47	theme	crude	336:340	arg1	MOP-100					359:365	MOP-100	359:365	MOP-100	359:365	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	47	theme	crude	336:340	arg1	polysaccharides					342:356	crude polysaccharides	336:356	crude polysaccharides (MOP-100) from the processed root of Morinda officinalis	336:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	0	48	theme	processed	47:55	arg1	officinalis					66:76	processed Morindae officinalis	47:76	processed Morindae officinalis	47:76	The chemical character of polysaccharides from processed Morindae officinalis and their effects on anti-liver damage.
31465803	1	49	used	used	190:193	arg2	root					170:173	its root	166:173	its root	166:173	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	1	49	used	used	190:193	arg2	officinalis					146:156	The medicinal plant Morinda officinalis	118:156	The medicinal plant Morinda officinalis How	118:160	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	2	50	from	root	387:390	arg1	MOP-100					359:365	MOP-100	359:365	MOP-100	359:365	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	50	from	root	387:390	arg1	composition					293:303	the chemical composition	280:303	the chemical composition	280:303	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	50	from	root	387:390	arg1	effect					326:331	liver-protective effect	309:331	liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro	309:482	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	2	50	from	root	387:390	arg1	polysaccharides					342:356	crude polysaccharides	336:356	crude polysaccharides (MOP-100) from the processed root of Morinda officinalis	336:413	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	1	51	theme	medicinal	122:130	arg1	officinalis					146:156	The medicinal plant Morinda officinalis	118:156	The medicinal plant Morinda officinalis How	118:160	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	7	52	dep	inhibited	949:957	arg1	improved					1019:1026	improved	1019:1026	improved the hepatic injury induced by ConA in mice	1019:1069	MOP-100 significantly inhibited the infiltration of neutrophils and macrophages into liver, improved the hepatic injury induced by ConA in mice.
31465803	6	53	theme	2071 Da	918:924	arg1	weight					908:913	molecular weight	898:913	molecular weight of 2071 Da	898:924	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	7	54	theme	hepatic	1032:1038	arg1	injury					1040:1045	the hepatic injury	1028:1045	the hepatic injury induced by ConA in mice	1028:1069	MOP-100 significantly inhibited the infiltration of neutrophils and macrophages into liver, improved the hepatic injury induced by ConA in mice.
31465803	2	55	theme	present	246:252	arg1	study					254:258	the present study	242:258	the present study	242:258	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	1	56	theme	plant	132:136	arg1	officinalis					146:156	The medicinal plant Morinda officinalis	118:156	The medicinal plant Morinda officinalis How	118:160	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	11	57	theme	liver	1538:1542	arg1	disease					1544:1550	immune-mediated liver disease	1522:1550	immune-mediated liver disease in the future	1522:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	8	58	theme	liver	1113:1117	arg1	cells					1123:1127	human liver LO2 cells	1107:1127	human liver LO2 cells	1107:1127	It stimulated the proliferation of human liver LO2 cells and decreased the cell death induced by ConA.
31465803	11	59	theme	officinalis	1413:1423	arg1	polysaccharides					1425:1439	Morinda officinalis polysaccharides	1405:1439	Morinda officinalis polysaccharides	1405:1439	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	6	60	with	fructooligosaccharide	871:891	arg1	weight					908:913	molecular weight	898:913	molecular weight of 2071 Da	898:924	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	1	61	theme	Morinda	138:144	arg1	officinalis					146:156	The medicinal plant Morinda officinalis	118:156	The medicinal plant Morinda officinalis How	118:160	The medicinal plant Morinda officinalis How and its root have long been used in traditional medicines in China.
31465803	3	62	with	precipitated	568:579	arg1	ethanol					586:592	ethanol	586:592	ethanol	586:592	The processed root of the herbal medicine was extracted with water at 100 °C, then precipitated with ethanol, dialyzed and freeze-dried to obtain MOP-100.
31465803	8	63	theme	LO2	1119:1121	arg1	cells					1123:1127	human liver LO2 cells	1107:1127	human liver LO2 cells	1107:1127	It stimulated the proliferation of human liver LO2 cells and decreased the cell death induced by ConA.
31465803	6	64	contain	contained	846:854	arg2	fructooligosaccharide					871:891	an inulin-type fructooligosaccharide	856:891	an inulin-type fructooligosaccharide with molecular weight of 2071 Da	856:924	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	6	64	contain	contained	846:854	arg1	MOP-60					832:837	MOP-60	832:837	MOP-60	832:837	MOP-60 mainly contained an inulin-type fructooligosaccharide with molecular weight of 2071 Da.
31465803	3	65	theme	processed	489:497	arg1	root					499:502	The processed root	485:502	The processed root of the herbal medicine	485:525	The processed root of the herbal medicine was extracted with water at 100 °C, then precipitated with ethanol, dialyzed and freeze-dried to obtain MOP-100.
31465803	2	66	theme	-induced	444:451	arg1	damage					459:464	Concanavalin A (ConA)-induced liver damage	423:464	Concanavalin A (ConA)-induced liver damage	423:464	The aims of the present study were to investigate the chemical composition and liver-protective effect of crude polysaccharides (MOP-100) from the processed root of Morinda officinalis against Concanavalin A (ConA)-induced liver damage in vivo and vitro.
31465803	11	67	from	future	1559:1564	arg1	treatment					1509:1517	treatment	1509:1517	treatment of immune-mediated liver disease in the future	1509:1564	These results suggested Morinda officinalis polysaccharides exert significant hepatoprotective effect, and it might be used for treatment of immune-mediated liver disease in the future.
31465803	5	68	dep	polysaccharides	793:807	arg1	MOP-60					809:814	MOP-60	809:814	MOP-60	809:814	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	5	68	dep	polysaccharides	793:807	arg1	polysaccharides					793:807	two crude polysaccharides MOP-60 and MOP-60-100	783:829	two crude polysaccharides MOP-60 and MOP-60-100	783:829	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	5	68	dep	polysaccharides	793:807	arg1	MOP-60-100					820:829	MOP-60-100	820:829	MOP-60-100	820:829	However, the processed root was extracted consecutively at 60 °C and 100 °C to obtain two crude polysaccharides MOP-60 and MOP-60-100.
31465803	7	69	theme	neutrophils	979:989	arg1	infiltration					963:974	the infiltration	959:974	the infiltration of neutrophils and macrophages into liver	959:1016	MOP-100 significantly inhibited the infiltration of neutrophils and macrophages into liver, improved the hepatic injury induced by ConA in mice.
31465803	4	70	theme	molecular	644:652	arg1	weight					654:659	The molecular weight	640:659	The molecular weight of MOP-100	640:670	The molecular weight of MOP-100 was a wide distribution.
31465803	4	70	theme	molecular	644:652	arg1	distribution					683:694	a wide distribution	676:694	a wide distribution	676:694	The molecular weight of MOP-100 was a wide distribution.
31465803	8	71	theme	cell	1147:1150	arg1	death					1152:1156	the cell death	1143:1156	the cell death induced by ConA	1143:1172	It stimulated the proliferation of human liver LO2 cells and decreased the cell death induced by ConA.
30165147	0	0	theme	cassava	78:84	arg1	composites					100:109	cassava starch hybrid composites	78:109	cassava starch hybrid composites	78:109	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	6	1	theme	calcium	1009:1015	arg1	clumps					1017:1022	the calcium clumps	1005:1022	the calcium clumps in the matrix	1005:1036	The SEM micrographs indicate that the CNF-ramie is bound in a matrix and the PCC is weakly bound in the tapioca starch matrix mainly due to the calcium clumps in the matrix.
30165147	1	2	theme	hybrid	243:248	arg1	composites					250:259	nano PCC tapioca starch hybrid composites	219:259	nano PCC tapioca starch hybrid composites	219:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	5	3	theme	PCC	759:761	arg1	addition					733:740	The addition	729:740	The addition of CNF-ramie and PCC in nanocomposites	729:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	6	4	theme	tapioca	969:975	arg1	matrix					984:989	the tapioca starch matrix	965:989	the tapioca starch matrix	965:989	The SEM micrographs indicate that the CNF-ramie is bound in a matrix and the PCC is weakly bound in the tapioca starch matrix mainly due to the calcium clumps in the matrix.
30165147	0	5	theme	hybrid	93:98	arg1	composites					100:109	cassava starch hybrid composites	78:109	cassava starch hybrid composites	78:109	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	5	6	from	nanocomposites	766:779	arg1	addition					733:740	The addition	729:740	The addition of CNF-ramie and PCC in nanocomposites	729:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	3	7	theme	samples	548:554	arg1	FTIR					500:503	FTIR	500:503	FTIR	500:503	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	3	7	theme	samples	548:554	arg1	morphology					524:533	the morphology	520:533	the morphology of composite samples have been analyzed through SEM	520:585	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	3	7	theme	samples	548:554	arg1	SEM					495:497	SEM	495:497	SEM	495:497	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	3	7	theme	samples	548:554	arg1	TGA					511:513	TGA	511:513	TGA	511:513	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	3	7	theme	samples	548:554	arg1	XRD					506:508	XRD	506:508	XRD	506:508	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	0	8	theme	starch	86:91	arg1	composites					100:109	cassava starch hybrid composites	78:109	cassava starch hybrid composites	78:109	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	4	9	theme	strength	658:665	arg1	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	4	10	theme	highest	642:648	arg1	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	3	11	theme	Physical	426:433	arg1	properties					460:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	1	12	theme	CNF	172:174	arg1	synthesis					138:146	synthesis	138:146	synthesis	138:146	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	1	12	theme	CNF	172:174	arg1	characterization					152:167	characterization	152:167	characterization	152:167	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	5	13	theme	moisture	795:802	arg1	absorption					804:813	moisture absorption	795:813	moisture absorption	795:813	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	3	14	theme	mechanical	436:445	arg1	properties					460:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	5	15	theme	absorption	804:813	arg1	properties					853:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	6	16	theme	starch	977:982	arg1	matrix					984:989	the tapioca starch matrix	965:989	the tapioca starch matrix	965:989	The SEM micrographs indicate that the CNF-ramie is bound in a matrix and the PCC is weakly bound in the tapioca starch matrix mainly due to the calcium clumps in the matrix.
30165147	4	17	theme	tensile	650:656	arg1	strength					658:665	tensile strength	650:665	tensile strength	650:665	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	6	18	from	clumps	1017:1022	arg1	matrix					1031:1036	the matrix	1027:1036	the matrix	1027:1036	The SEM micrographs indicate that the CNF-ramie is bound in a matrix and the PCC is weakly bound in the tapioca starch matrix mainly due to the calcium clumps in the matrix.
30165147	3	19	theme	composite	538:546	arg1	samples					548:554	composite samples	538:554	composite samples have been analyzed through SEM	538:585	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	1	20	theme	ramie	190:194	arg1	fibers					196:201	ramie fibers	190:201	ramie fibers reinforced with nano PCC tapioca starch hybrid composites	190:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	5	21	theme	crystallinity	816:828	arg1	properties					853:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	6	22	theme	SEM	869:871	arg1	micrographs					873:883	The SEM micrographs	865:883	The SEM micrographs	865:883	The SEM micrographs indicate that the CNF-ramie is bound in a matrix and the PCC is weakly bound in the tapioca starch matrix mainly due to the calcium clumps in the matrix.
30165147	1	23	dep	synthesis	138:146	arg1	the					134:136	the	134:136	the	134:136	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	4	24	theme	crystallinity	671:683	arg1	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	0	25	theme	nanofibers	44:53	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	0	25	theme	nanofibers	44:53	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	4	26	contain	has	634:636	arg1	sample					627:632	the CS/4CNF/6PCC sample	610:632	the CS/4CNF/6PCC sample	610:632	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	4	26	contain	has	634:636	arg2	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	5	27	theme	thermal	835:841	arg1	stability					843:851	thermal stability	835:851	thermal stability	835:851	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	0	28	theme	cellulose	34:42	arg1	nanofibers					44:53	cellulose nanofibers	34:53	cellulose nanofibers (CNF)	34:59	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	0	28	theme	cellulose	34:42	arg1	CNF					56:58	CNF	56:58	CNF	56:58	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	5	29	theme	stability	843:851	arg1	properties					853:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	5	30	from	addition	733:740	arg1	nanocomposites					766:779	nanocomposites	766:779	nanocomposites	766:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	5	31	theme	increased	785:793	arg1	properties					853:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	5	32	from	PCC	759:761	arg1	nanocomposites					766:779	nanocomposites	766:779	nanocomposites	766:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	4	33	theme	12.84 Mpa	694:702	arg1	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	2	34	theme	chemical-ultrasonication	294:317	arg1	process					319:325	chemical-ultrasonication process	294:325	chemical-ultrasonication process	294:325	CNF-ramie was prepared by using chemical-ultrasonication process, while the nano-composites were made by utilizing a casting solution and glycerol as plasticizers.
30165147	5	35	contain	has	781:783	arg1	addition					733:740	The addition	729:740	The addition of CNF-ramie and PCC in nanocomposites	729:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	5	35	contain	has	781:783	arg2	properties					853:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	increased moisture absorption, crystallinity, and thermal stability properties	785:862	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	4	36	theme	%	713:713	arg1	index					685:689	the highest tensile strength and crystallinity index	638:689	the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively	638:726	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	0	37	dep	ramie	61:65	arg1	reinforced					67:76	reinforced	67:76	ramie reinforced cassava starch hybrid composites	61:109	Synthesis and characterization of cellulose nanofibers (CNF) ramie reinforced cassava starch hybrid composites.
30165147	1	38	theme	nano	219:222	arg1	composites					250:259	nano PCC tapioca starch hybrid composites	219:259	nano PCC tapioca starch hybrid composites	219:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	1	39	theme	PCC	224:226	arg1	composites					250:259	nano PCC tapioca starch hybrid composites	219:259	nano PCC tapioca starch hybrid composites	219:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	5	40	from	CNF-ramie	745:753	arg1	nanocomposites					766:779	nanocomposites	766:779	nanocomposites	766:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	4	41	theme	CS/4CNF/6PCC	614:625	arg1	sample					627:632	the CS/4CNF/6PCC sample	610:632	the CS/4CNF/6PCC sample	610:632	The results show that the CS/4CNF/6PCC sample has the highest tensile strength and crystallinity index of 12.84 Mpa and 30.76% respectively.
30165147	2	42	theme	casting	379:385	arg1	glycerol					400:407	glycerol	400:407	glycerol	400:407	CNF-ramie was prepared by using chemical-ultrasonication process, while the nano-composites were made by utilizing a casting solution and glycerol as plasticizers.
30165147	2	42	theme	casting	379:385	arg1	solution					387:394	a casting solution	377:394	a casting solution	377:394	CNF-ramie was prepared by using chemical-ultrasonication process, while the nano-composites were made by utilizing a casting solution and glycerol as plasticizers.
30165147	2	42	theme	casting	379:385	arg1	plasticizers					412:423	plasticizers	412:423	plasticizers	412:423	CNF-ramie was prepared by using chemical-ultrasonication process, while the nano-composites were made by utilizing a casting solution and glycerol as plasticizers.
30165147	3	43	theme	thermal	452:458	arg1	properties					460:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties	426:469	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	1	44	theme	tapioca	228:234	arg1	composites					250:259	nano PCC tapioca starch hybrid composites	219:259	nano PCC tapioca starch hybrid composites	219:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
30165147	5	45	theme	CNF-ramie	745:753	arg1	addition					733:740	The addition	729:740	The addition of CNF-ramie and PCC in nanocomposites	729:779	The addition of CNF-ramie and PCC in nanocomposites has increased moisture absorption, crystallinity, and thermal stability properties.
30165147	3	46	dep	samples	548:554	arg1	analyzed					566:573	analyzed	566:573	have been analyzed through SEM	556:585	Physical, mechanical, and thermal properties are characterized using SEM, FTIR, XRD, TGA, and the morphology of composite samples have been analyzed through SEM.
30165147	1	47	theme	starch	236:241	arg1	composites					250:259	nano PCC tapioca starch hybrid composites	219:259	nano PCC tapioca starch hybrid composites	219:259	This study focuses on the synthesis and characterization of CNF obtained from ramie fibers reinforced with nano PCC tapioca starch hybrid composites.
31381912	8	0	theme	large	1397:1401	arg1	proportion					1403:1412	a large proportion	1395:1412	a large proportion	1395:1412	Among the monosaccharides tested, the EPs contained five kinds, of which galactose and xylose accounted for a large proportion.
31381912	5	1	theme	HPLC	892:895	arg1	method					897:902	HPLC method	892:902	HPLC method	892:902	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	6	2	theme	antioxidant	988:998	arg1	capacity					1000:1007	the total antioxidant capacity	978:1007	the total antioxidant capacity	978:1007	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	3	theme	DPPH	1010:1013	arg1	capacity					1034:1041	DPPH radical scavenging capacity	1010:1041	DPPH radical scavenging capacity	1010:1041	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	0	4	from	study	12:16	arg1	compositions					40:51	the monosaccharide compositions	21:51	the monosaccharide compositions	21:51	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	6	5	from	capacity	1000:1007	arg1	α-amylase					1100:1108	α-amylase	1100:1108	α-amylase	1100:1108	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	6	theme	inhibition	1079:1088	arg1	effect					1090:1095	inhibition effect	1079:1095	inhibition effect	1079:1095	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	7	theme	total	982:986	arg1	capacity					1000:1007	the total antioxidant capacity	978:1007	the total antioxidant capacity	978:1007	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	8	from	ability	1067:1073	arg1	α-amylase					1100:1108	α-amylase	1100:1108	α-amylase	1100:1108	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	3	9	theme	simultaneous	534:545	arg1	study					547:551	the simultaneous study	530:551	the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	530:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	5	10	theme	IPs	956:958	arg1	compositions					936:947	the monosaccharide compositions	917:947	the monosaccharide compositions of the IPs and EPs	917:966	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	8	11	contain	contained	1329:1337	arg1	EPs					1325:1327	the EPs	1321:1327	the EPs	1321:1327	Among the monosaccharides tested, the EPs contained five kinds, of which galactose and xylose accounted for a large proportion.
31381912	8	11	contain	contained	1329:1337	arg2	kinds					1344:1348	five kinds	1339:1348	five kinds	1339:1348	Among the monosaccharides tested, the EPs contained five kinds, of which galactose and xylose accounted for a large proportion.
31381912	9	12	theme	activity	1483:1490	arg1	inhibition					1459:1468	inhibition	1459:1468	inhibition of α-amylase activity	1459:1490	ICPs showed better antioxidant activity and inhibition of α-amylase activity.
31381912	9	12	theme	activity	1483:1490	arg1	activity					1446:1453	better antioxidant activity	1427:1453	better antioxidant activity	1427:1453	ICPs showed better antioxidant activity and inhibition of α-amylase activity.
31381912	10	13	theme	comatus	1606:1612	arg1	development					1588:1598	the deep development	1579:1598	the deep development of C. comatus	1579:1612	This article innovatively studied the IPs and EPs, which could lay the foundation for the deep development of C. comatus.
31381912	5	14	theme	EPs	964:966	arg1	compositions					936:947	the monosaccharide compositions	917:947	the monosaccharide compositions of the IPs and EPs	917:966	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	6	15	dep	IDPs	1192:1195	arg1	EDPs					1198:1201	EDPs	1198:1201	EDPs	1198:1201	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	16	theme	crude	1122:1126	arg1	polysaccharides					1128:1142	crude polysaccharides	1122:1142	crude polysaccharides (ICPs, ECPs)	1122:1155	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	2	17	theme	comatus	428:434	arg1	polysaccharides					462:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	6	18	theme	scavenging	1056:1065	arg1	ability					1067:1073	·OH radical scavenging ability	1044:1073	·OH radical scavenging ability	1044:1073	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	0	19	theme	intracellular	106:118	arg1	polysaccharides					138:152	intracellular and extracellular polysaccharides	106:152	intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus	106:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	6	20	from	capacity	1034:1041	arg1	α-amylase					1100:1108	α-amylase	1100:1108	α-amylase	1100:1108	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	4	21	theme	activity	784:791	arg1	inhibition					760:769	inhibition	760:769	inhibition of α-amylase activity in vitro of IPs and EPs	760:815	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	21	theme	activity	784:791	arg1	compositions					717:728	the monosaccharide compositions	698:728	the monosaccharide compositions	698:728	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	21	theme	activity	784:791	arg1	activities					745:754	antioxidative activities	731:754	antioxidative activities	731:754	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	2	22	theme	antioxidant	395:405	arg1	activity					407:414	antioxidant activity	395:414	antioxidant activity	395:414	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	2	23	theme	fruit	449:453	arg1	polysaccharides					462:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	6	24	theme	scavenging	1023:1032	arg1	capacity					1034:1041	DPPH radical scavenging capacity	1010:1041	DPPH radical scavenging capacity	1010:1041	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	5	25	theme	pre-column	852:861	arg1	derivatization					863:876	the PMP pre-column derivatization	844:876	the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs	844:966	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	0	26	theme	Comparative	0:10	arg1	activities					83:92	hypoglycemic activities	70:92	hypoglycemic activities in vitro	70:101	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	0	26	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on the monosaccharide compositions	0:51	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	0	26	theme	Comparative	0:10	arg1	antioxidant					54:64	antioxidant	54:64	antioxidant	54:64	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	1	27	theme	unique	289:294	arg1	role					296:299	its unique role	285:299	its unique role caused the widespread concern of researchers at home and abroad	285:363	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	3	28	theme	extracellular	574:586	arg1	polysaccharides					588:602	intracellular and extracellular polysaccharides	556:602	intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	556:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	4	29	theme	α-amylase	774:782	arg1	activity					784:791	α-amylase activity	774:791	α-amylase activity	774:791	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	6	30	theme	radical	1048:1054	arg1	ability					1067:1073	·OH radical scavenging ability	1044:1073	·OH radical scavenging ability	1044:1073	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	4	31	theme	antioxidative	731:743	arg1	activities					745:754	antioxidative activities	731:754	antioxidative activities	731:754	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	0	32	theme	polysaccharides	138:152	arg1	activities					83:92	hypoglycemic activities	70:92	hypoglycemic activities in vitro	70:101	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	0	32	theme	polysaccharides	138:152	arg1	study					12:16	Comparative study	0:16	Comparative study on the monosaccharide compositions	0:51	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	0	32	theme	polysaccharides	138:152	arg1	antioxidant					54:64	antioxidant	54:64	antioxidant	54:64	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	3	33	from	article	519:525	arg1	study					547:551	the simultaneous study	530:551	the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	530:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	9	34	theme	antioxidant	1434:1444	arg1	activity					1446:1453	better antioxidant activity	1427:1453	better antioxidant activity	1427:1453	ICPs showed better antioxidant activity and inhibition of α-amylase activity.
31381912	6	35	theme	·OH	1044:1046	arg1	ability					1067:1073	·OH radical scavenging ability	1044:1073	·OH radical scavenging ability	1044:1073	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	4	36	theme	IPs	805:807	arg1	inhibition					760:769	inhibition	760:769	inhibition of α-amylase activity in vitro of IPs and EPs	760:815	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	36	theme	IPs	805:807	arg1	compositions					717:728	the monosaccharide compositions	698:728	the monosaccharide compositions	698:728	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	36	theme	IPs	805:807	arg1	activities					745:754	antioxidative activities	731:754	antioxidative activities	731:754	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	0	37	theme	extracellular	124:136	arg1	polysaccharides					138:152	intracellular and extracellular polysaccharides	106:152	intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus	106:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	6	38	theme	deproteinized	1161:1173	arg1	IDPs					1192:1195	IDPs	1192:1195	IDPs	1192:1195	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	38	theme	deproteinized	1161:1173	arg1	polysaccharides					1175:1189	deproteinized polysaccharides	1161:1189	deproteinized polysaccharides (IDPs, EDPs)	1161:1202	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	3	39	theme	intracellular	556:568	arg1	polysaccharides					588:602	intracellular and extracellular polysaccharides	556:602	intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	556:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	1	40	theme	important	224:232	arg1	ingredients					241:251	the important active ingredients	220:251	the important active ingredients of medical fungi	220:268	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	0	41	theme	liquid	157:162	arg1	comatus					183:189	liquid fermented Coprinus comatus	157:189	liquid fermented Coprinus comatus	157:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	3	42	theme	liquid	607:612	arg1	comatus					627:633	liquid fermented C. comatus	607:633	liquid fermented C. comatus (IPs, EPs)	607:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	3	42	theme	liquid	607:612	arg1	IPs					636:638	IPs	636:638	IPs	636:638	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	6	43	theme	polysaccharides	1175:1189	arg1	ability					1067:1073	·OH radical scavenging ability	1044:1073	·OH radical scavenging ability	1044:1073	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	43	theme	polysaccharides	1175:1189	arg1	capacity					1034:1041	DPPH radical scavenging capacity	1010:1041	DPPH radical scavenging capacity	1010:1041	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	43	theme	polysaccharides	1175:1189	arg1	capacity					1000:1007	the total antioxidant capacity	978:1007	the total antioxidant capacity	978:1007	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	43	theme	polysaccharides	1175:1189	arg1	effect					1090:1095	inhibition effect	1079:1095	inhibition effect	1079:1095	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	1	44	theme	active	234:239	arg1	ingredients					241:251	the important active ingredients	220:251	the important active ingredients of medical fungi	220:268	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	0	45	theme	monosaccharide	25:38	arg1	compositions					40:51	the monosaccharide compositions	21:51	the monosaccharide compositions	21:51	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	1	46	theme	widespread	312:321	arg1	concern					323:329	the widespread concern	308:329	the widespread concern of researchers	308:344	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	3	47	theme	fermented	614:622	arg1	comatus					627:633	liquid fermented C. comatus	607:633	liquid fermented C. comatus (IPs, EPs)	607:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	3	47	theme	fermented	614:622	arg1	IPs					636:638	IPs	636:638	IPs	636:638	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	1	48	theme	ingredients	241:251	arg1	one					213:215	one	213:215	one	213:215	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	1	48	theme	ingredients	241:251	arg1	ingredients					241:251	the important active ingredients	220:251	the important active ingredients of medical fungi	220:268	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	2	49	theme	polysaccharides	462:476	arg1	structure					381:389	structure	381:389	structure	381:389	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	2	49	theme	polysaccharides	462:476	arg1	activity					407:414	antioxidant activity	395:414	antioxidant activity	395:414	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	0	50	theme	Coprinus	174:181	arg1	comatus					183:189	liquid fermented Coprinus comatus	157:189	liquid fermented Coprinus comatus	157:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	7	51	located	detected	1270:1277	arg1	IPs					1282:1284	IPs	1282:1284	IPs	1282:1284	Only three kinds of monosaccharides were detected in IPs.
31381912	7	51	located	detected	1270:1277	arg2	kinds					1240:1244	Only three kinds	1229:1244	Only three kinds of monosaccharides	1229:1263	Only three kinds of monosaccharides were detected in IPs.
31381912	3	52	theme	polysaccharides	588:602	arg1	study					547:551	the simultaneous study	530:551	the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	530:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	4	53	theme	monosaccharide	702:715	arg1	compositions					717:728	the monosaccharide compositions	698:728	the monosaccharide compositions	698:728	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	2	54	theme	bodies	455:460	arg1	polysaccharides					462:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Coprinus comatus (C. comatus) fruit bodies polysaccharides	419:476	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	0	55	theme	fermented	164:172	arg1	comatus					183:189	liquid fermented Coprinus comatus	157:189	liquid fermented Coprinus comatus	157:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	2	56	dep	comatus	428:434	arg1	comatus					440:446	C. comatus	437:446	C. comatus	437:446	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	7	57	theme	monosaccharides	1249:1263	arg1	kinds					1240:1244	Only three kinds	1229:1244	Only three kinds of monosaccharides	1229:1263	Only three kinds of monosaccharides were detected in IPs.
31381912	1	58	theme	researchers	334:344	arg1	concern					323:329	the widespread concern	308:329	the widespread concern of researchers	308:344	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	4	59	theme	EPs	813:815	arg1	inhibition					760:769	inhibition	760:769	inhibition of α-amylase activity in vitro of IPs and EPs	760:815	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	59	theme	EPs	813:815	arg1	compositions					717:728	the monosaccharide compositions	698:728	the monosaccharide compositions	698:728	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	4	59	theme	EPs	813:815	arg1	activities					745:754	antioxidative activities	731:754	antioxidative activities	731:754	Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs.
31381912	9	60	theme	α-amylase	1473:1481	arg1	activity					1483:1490	α-amylase activity	1473:1490	α-amylase activity	1473:1490	ICPs showed better antioxidant activity and inhibition of α-amylase activity.
31381912	6	61	theme	radical	1015:1021	arg1	capacity					1034:1041	DPPH radical scavenging capacity	1010:1041	DPPH radical scavenging capacity	1010:1041	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	0	62	theme	comatus	183:189	arg1	polysaccharides					138:152	intracellular and extracellular polysaccharides	106:152	intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus	106:189	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	6	63	dep	polysaccharides	1128:1142	arg1	ICPs					1145:1148	ICPs	1145:1148	ICPs	1145:1148	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	63	dep	polysaccharides	1128:1142	arg1	ECPs					1151:1154	ECPs	1151:1154	ECPs	1151:1154	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	2	64	dep	structure	381:389	arg1	the					377:379	the	377:379	the	377:379	Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported.
31381912	6	65	from	effect	1090:1095	arg1	α-amylase					1100:1108	α-amylase	1100:1108	α-amylase	1100:1108	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	5	66	theme	monosaccharide	921:934	arg1	compositions					936:947	the monosaccharide compositions	917:947	the monosaccharide compositions of the IPs and EPs	917:966	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	1	67	theme	medical	256:262	arg1	fungi					264:268	medical fungi	256:268	medical fungi	256:268	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	0	68	theme	hypoglycemic	70:81	arg1	activities					83:92	hypoglycemic activities	70:92	hypoglycemic activities in vitro	70:101	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	0	68	theme	hypoglycemic	70:81	arg1	study					12:16	Comparative study	0:16	Comparative study on the monosaccharide compositions	0:51	Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus.
31381912	3	69	dep	IPs	636:638	arg1	EPs					641:643	EPs	641:643	EPs	641:643	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	3	70	theme	C.	624:625	arg1	comatus					627:633	liquid fermented C. comatus	607:633	liquid fermented C. comatus (IPs, EPs)	607:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	3	70	theme	C.	624:625	arg1	IPs					636:638	IPs	636:638	IPs	636:638	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	9	71	theme	better	1427:1432	arg1	activity					1446:1453	better antioxidant activity	1427:1453	better antioxidant activity	1427:1453	ICPs showed better antioxidant activity and inhibition of α-amylase activity.
31381912	5	72	theme	PMP	848:850	arg1	derivatization					863:876	the PMP pre-column derivatization	844:876	the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs	844:966	In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs.
31381912	1	73	theme	fungi	264:268	arg1	ingredients					241:251	the important active ingredients	220:251	the important active ingredients of medical fungi	220:268	Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad.
31381912	10	74	theme	deep	1583:1586	arg1	development					1588:1598	the deep development	1579:1598	the deep development of C. comatus	1579:1612	This article innovatively studied the IPs and EPs, which could lay the foundation for the deep development of C. comatus.
31381912	6	75	theme	polysaccharides	1128:1142	arg1	ability					1067:1073	·OH radical scavenging ability	1044:1073	·OH radical scavenging ability	1044:1073	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	75	theme	polysaccharides	1128:1142	arg1	capacity					1034:1041	DPPH radical scavenging capacity	1010:1041	DPPH radical scavenging capacity	1010:1041	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	75	theme	polysaccharides	1128:1142	arg1	capacity					1000:1007	the total antioxidant capacity	978:1007	the total antioxidant capacity	978:1007	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	6	75	theme	polysaccharides	1128:1142	arg1	effect					1090:1095	inhibition effect	1079:1095	inhibition effect	1079:1095	Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared.
31381912	3	76	theme	comatus	627:633	arg1	polysaccharides					588:602	intracellular and extracellular polysaccharides	556:602	intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs)	556:644	However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far.
31381912	10	77	dep	IPs	1531:1533	arg1	the					1527:1529	the	1527:1529	the	1527:1529	This article innovatively studied the IPs and EPs, which could lay the foundation for the deep development of C. comatus.
31052416	10	0	theme	cancer	1655:1660	arg1	treatment					1662:1670	cancer treatment	1655:1670	cancer treatment	1655:1670	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	6	1	theme	hydrothermal	987:998	arg1	IFREMER					1005:1011	deep-sea hydrothermal vent IFREMER	978:1011	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	974:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	6	2	theme	able	1116:1119	arg1	strains					1108:1114	new strains	1104:1114	new strains able to excrete EPS endowed with GAG-like structural features	1104:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	6	3	theme	structural	1158:1167	arg1	features					1169:1176	GAG-like structural features	1149:1176	GAG-like structural features	1149:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	1	4	theme	protective	273:282	arg1	microenvironment					284:299	a protective microenvironment	271:299	a protective microenvironment	271:299	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	4	5	from	rich	668:671	arg1	hexosamines					676:686	hexosamines	676:686	hexosamines	676:686	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	4	5	from	rich	668:671	arg1	acids					699:703	uronic acids	692:703	uronic acids	692:703	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	6	6	theme	deep-sea	978:985	arg1	IFREMER					1005:1011	deep-sea hydrothermal vent IFREMER	978:1011	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	974:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	6	7	theme	GAG-like	1149:1156	arg1	features					1169:1176	GAG-like structural features	1149:1176	GAG-like structural features	1149:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	9	8	theme	atypical	1502:1509	arg1	patterns					1525:1532	atypical compositional patterns	1502:1532	atypical compositional patterns	1502:1532	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	7	9	from	scale	1315:1319	arg1	production					1286:1295	EPS production	1282:1295	EPS production at the laboratory scale	1282:1319	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	3	10	with	composition	455:465	arg1	presence					476:483	the presence	472:483	the presence of particular chemical features	472:515	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	6	11	theme	Sea	1064:1066	arg1	Exploitation					1044:1055	Exploitation	1044:1055	Exploitation of the Sea	1044:1066	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	7	12	contain	containing	1223:1232	arg1	collection					1212:1221	our whole collection	1202:1221	our whole collection containing 692 strains	1202:1244	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	7	12	contain	containing	1223:1232	arg2	strains					1238:1244	692 strains	1234:1244	692 strains	1234:1244	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	0	13	theme	Deep-sea	0:7	arg1	Bacteria					27:34	Deep-sea Hydrothermal Vent Bacteria	0:34	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.	0:95	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.
31052416	9	14	theme	patterns	1525:1532	arg1	presence					1490:1497	the presence	1486:1497	the presence of atypical compositional patterns	1486:1532	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	8	15	theme	16S	1373:1375	arg1	phylogeny					1382:1390	16S rDNA phylogeny	1373:1390	16S rDNA phylogeny	1373:1390	EPS-producing strains were identified according to 16S rDNA phylogeny.
31052416	4	16	theme	uronic	692:697	arg1	acids					699:703	uronic acids	692:703	uronic acids	692:703	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	2	17	theme	structural	311:320	arg1	component					322:330	The main structural component	302:330	The main structural component of this complex network	302:354	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	3	18	theme	chemical	499:506	arg1	features					508:515	particular chemical features	488:515	particular chemical features	488:515	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	5	19	theme	biological	829:838	arg1	activities					840:849	biological activities	829:849	biological activities mimicking those known for glycosaminoglycans (GAG)	829:900	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	9	20	theme	Chemical	1393:1400	arg1	characterization					1402:1417	Chemical characterization	1393:1417	Chemical characterization of the obtained EPS	1393:1437	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	6	21	theme	collection	1069:1078	arg1	exploration					959:969	the exploration	955:969	the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	955:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	2	22	theme	main	306:309	arg1	component					322:330	The main structural component	302:330	The main structural component of this complex network	302:354	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	9	23	theme	high	1457:1460	arg1	diversity					1471:1479	their high chemical diversity	1451:1479	their high chemical diversity	1451:1479	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	3	24	theme	features	508:515	arg1	presence					476:483	the presence	472:483	the presence of particular chemical features	472:515	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	0	25	theme	Hydrothermal	9:20	arg1	Bacteria					27:34	Deep-sea Hydrothermal Vent Bacteria	0:34	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.	0:95	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.
31052416	7	26	theme	EPS	1282:1284	arg1	production					1286:1295	EPS production	1282:1295	EPS production at the laboratory scale	1282:1319	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	6	27	dep	EPS	1132:1134	arg1	endowed					1136:1142	endowed	1136:1142	endowed	1136:1142	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	3	28	theme	EPS	451:453	arg1	composition					455:465	EPS composition	451:465	EPS composition with the presence of particular chemical features	451:515	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	10	29	theme	potential	1556:1564	arg1	treatment					1662:1670	cancer treatment	1655:1670	cancer treatment	1655:1670	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	10	29	theme	potential	1556:1564	arg1	bioactives					1566:1575	potential bioactives	1556:1575	potential bioactives	1556:1575	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	10	29	theme	potential	1556:1564	arg1	medicines					1641:1649	regenerative medicines	1628:1649	regenerative medicines	1628:1649	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	9	30	theme	compositional	1511:1523	arg1	patterns					1525:1532	atypical compositional patterns	1502:1532	atypical compositional patterns	1502:1532	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	2	31	theme	network	348:354	arg1	component					322:330	The main structural component	302:330	The main structural component of this complex network	302:354	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	8	32	theme	EPS-producing	1322:1334	arg1	strains					1336:1342	EPS-producing strains	1322:1342	EPS-producing strains	1322:1342	EPS-producing strains were identified according to 16S rDNA phylogeny.
31052416	3	33	theme	particular	488:497	arg1	features					508:515	particular chemical features	488:515	particular chemical features	488:515	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	8	34	theme	rDNA	1377:1380	arg1	phylogeny					1382:1390	16S rDNA phylogeny	1373:1390	16S rDNA phylogeny	1373:1390	EPS-producing strains were identified according to 16S rDNA phylogeny.
31052416	10	35	theme	applications	1604:1615	arg1	number					1583:1588	a number	1581:1588	a number of biomedical applications	1581:1615	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	2	36	theme	complex	340:346	arg1	network					348:354	this complex network	335:354	this complex network	335:354	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	9	37	theme	chemical	1462:1469	arg1	diversity					1471:1479	their high chemical diversity	1451:1479	their high chemical diversity	1451:1479	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	1	38	theme	extracellular	214:226	arg1	matrix					238:243	an extracellular polymeric matrix	211:243	an extracellular polymeric matrix conferring upon the cells a protective microenvironment	211:299	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	0	39	theme	Vent	22:25	arg1	Bacteria					27:34	Deep-sea Hydrothermal Vent Bacteria	0:34	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.	0:95	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.
31052416	9	40	theme	obtained	1426:1433	arg1	EPS					1435:1437	the obtained EPS	1422:1437	the obtained EPS	1422:1437	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	10	41	theme	biomedical	1593:1602	arg1	applications					1604:1615	biomedical applications	1593:1615	biomedical applications	1593:1615	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	1	42	theme	unique	123:128	arg1	strategy					130:137	a unique strategy	121:137	a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment	121:299	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	4	43	from	acids	699:703	arg1	rich					668:671	rich	668:671	rich	668:671	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	1	44	theme	polymeric	228:236	arg1	matrix					238:243	an extracellular polymeric matrix	211:243	an extracellular polymeric matrix conferring upon the cells a protective microenvironment	211:299	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	7	45	theme	collection	1212:1221	arg1	screening					1189:1197	the screening	1185:1197	the screening of our whole collection containing 692 strains	1185:1244	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	9	46	theme	EPS	1435:1437	arg1	characterization					1402:1417	Chemical characterization	1393:1417	Chemical characterization of the obtained EPS	1393:1437	Chemical characterization of the obtained EPS highlighted their high chemical diversity with the presence of atypical compositional patterns.
31052416	5	47	theme	interesting	784:794	arg1	properties					807:816	interesting functional properties	784:816	interesting functional properties	784:816	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	5	47	theme	interesting	784:794	arg1	activities					840:849	biological activities	829:849	biological activities mimicking those known for glycosaminoglycans (GAG)	829:900	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	6	48	dep	IFREMER	1005:1011	arg1	Institute					1030:1038	French Research Institute	1014:1038	French Research Institute for Exploitation of the Sea	1014:1066	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	4	49	theme	sulfate	730:736	arg1	groups					738:743	some sulfate groups	725:743	some sulfate groups	725:743	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	1	50	theme	matrix	238:243	arg1	synthesis					198:206	the synthesis	194:206	the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment	194:299	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	6	51	with	endowed	1136:1142	arg1	features					1169:1176	GAG-like structural features	1149:1176	GAG-like structural features	1149:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	4	52	theme	Deep-sea	593:600	arg1	bacteria					620:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	5	53	theme	functional	796:805	arg1	properties					807:816	interesting functional properties	784:816	interesting functional properties	784:816	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	5	53	theme	functional	796:805	arg1	activities					840:849	biological activities	829:849	biological activities mimicking those known for glycosaminoglycans (GAG)	829:900	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	6	54	theme	IFREMER	1005:1011	arg1	collection					1069:1078	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection	974:1078	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	974:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	6	55	theme	French	1014:1019	arg1	Institute					1030:1038	French Research Institute	1014:1038	French Research Institute for Exploitation of the Sea	1014:1066	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	4	56	from	hexosamines	676:686	arg1	rich					668:671	rich	668:671	rich	668:671	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	7	57	theme	laboratory	1304:1313	arg1	scale					1315:1319	the laboratory scale	1300:1319	the laboratory scale	1300:1319	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	3	58	theme	specific	547:554	arg1	conditions					556:565	the specific conditions	543:565	the specific conditions in which bacteria evolve	543:590	EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve.
31052416	7	59	theme	whole	1206:1210	arg1	collection					1212:1221	our whole collection	1202:1221	our whole collection containing 692 strains	1202:1244	After the screening of our whole collection containing 692 strains, 38 bacteria have been selected for EPS production at the laboratory scale.
31052416	4	60	theme	vent	615:618	arg1	bacteria					620:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	10	61	theme	regenerative	1628:1639	arg1	medicines					1641:1649	regenerative medicines	1628:1649	regenerative medicines	1628:1649	These EPS constitute potential bioactives for a number of biomedical applications, including regenerative medicines and cancer treatment.
31052416	6	62	theme	study	926:930	arg1	aim					907:909	The aim	903:909	The aim of the present study	903:930	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	2	63	theme	hydrophilic	390:400	arg1	macromolecules					402:415	high-molecular weight hydrophilic macromolecules	368:415	high-molecular weight hydrophilic macromolecules	368:415	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	2	63	theme	hydrophilic	390:400	arg1	exopolysaccharides					425:442	exopolysaccharides	425:442	namely exopolysaccharides (EPS)	418:448	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	4	64	theme	hydrothermal	602:613	arg1	bacteria					620:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria	593:627	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	6	65	theme	Research	1021:1028	arg1	Institute					1030:1038	French Research Institute	1014:1038	French Research Institute for Exploitation of the Sea	1014:1066	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	6	66	theme	present	918:924	arg1	study					926:930	the present study	914:930	the present study	914:930	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	2	67	theme	weight	383:388	arg1	macromolecules					402:415	high-molecular weight hydrophilic macromolecules	368:415	high-molecular weight hydrophilic macromolecules	368:415	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	2	67	theme	weight	383:388	arg1	exopolysaccharides					425:442	exopolysaccharides	425:442	namely exopolysaccharides (EPS)	418:448	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	5	68	theme	particular	753:762	arg1	composition					764:774	Such a particular composition	746:774	Such a particular composition	746:774	Such a particular composition ensures interesting functional properties, including biological activities mimicking those known for glycosaminoglycans (GAG).
31052416	2	69	theme	high-molecular	368:381	arg1	macromolecules					402:415	high-molecular weight hydrophilic macromolecules	368:415	high-molecular weight hydrophilic macromolecules	368:415	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	2	69	theme	high-molecular	368:381	arg1	exopolysaccharides					425:442	exopolysaccharides	425:442	namely exopolysaccharides (EPS)	418:448	The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS).
31052416	4	70	theme	rich	668:671	arg1	EPS					664:666	EPS	664:666	EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups	664:743	Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups.
31052416	0	71	theme	Exopolysaccharides	77:94	arg1	Source					41:46	a Source	39:46	a Source of Glycosaminoglycan-Mimetic Exopolysaccharides	39:94	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.
31052416	6	72	theme	new	1104:1106	arg1	strains					1108:1114	new strains	1104:1114	new strains able to excrete EPS endowed with GAG-like structural features	1104:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	1	73	theme	extreme	153:159	arg1	conditions					175:184	extreme environmental conditions	153:184	extreme environmental conditions	153:184	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
31052416	6	74	theme	bacteria	1083:1090	arg1	collection					1069:1078	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection	974:1078	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	974:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	0	75	theme	Glycosaminoglycan-Mimetic	51:75	arg1	Exopolysaccharides					77:94	Glycosaminoglycan-Mimetic Exopolysaccharides	51:94	Glycosaminoglycan-Mimetic Exopolysaccharides	51:94	Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides.
31052416	6	76	theme	vent	1000:1003	arg1	IFREMER					1005:1011	deep-sea hydrothermal vent IFREMER	978:1011	the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features	974:1176	The aim of the present study was to go further into the exploration of the deep-sea hydrothermal vent IFREMER (French Research Institute for Exploitation of the Sea) collection of bacteria to discover new strains able to excrete EPS endowed with GAG-like structural features.
31052416	1	77	theme	environmental	161:173	arg1	conditions					175:184	extreme environmental conditions	153:184	extreme environmental conditions	153:184	Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment.
30401646	2	0	theme	anti-aggregation	448:463	arg1	effects					465:471	anti-aggregation effects	448:471	anti-aggregation effects	448:471	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	1	theme	electron	582:589	arg1	TEM					603:605	TEM	603:605	TEM	603:605	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	1	theme	electron	582:589	arg1	microscopy					591:600	transmission electron microscopy	569:600	transmission electron microscopy (TEM) of fibril morphology	569:627	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	1	2	theme	different	172:180	arg1	samples					191:197	five different fucoidan samples	167:197	five different fucoidan samples	167:197	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	5	3	theme	Aβ1-42	1072:1077	arg1	neurotoxicity					1079:1091	Aβ1-42 neurotoxicity	1072:1091	Aβ1-42 neurotoxicity in Alzheimer's disease	1072:1114	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	0	4	from	study	12:16	arg1	activities					37:46	neuroprotective activities	21:46	neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida	21:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	2	5	theme	transmission	569:580	arg1	TEM					603:605	TEM	603:605	TEM	603:605	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	5	theme	transmission	569:580	arg1	microscopy					591:600	transmission electron microscopy	569:600	transmission electron microscopy (TEM) of fibril morphology	569:627	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	1	6	theme	fucoidan	182:189	arg1	samples					191:197	five different fucoidan samples	167:197	five different fucoidan samples	167:197	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	2	7	theme	morphology	618:627	arg1	kinetics					556:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	7	theme	morphology	618:627	arg1	TEM					603:605	TEM	603:605	TEM	603:605	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	7	theme	morphology	618:627	arg1	microscopy					591:600	transmission electron microscopy	569:600	transmission electron microscopy (TEM) of fibril morphology	569:627	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	5	8	theme	different	924:932	arg1	sources					942:948	different seaweed sources	924:948	different seaweed sources	924:948	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	3	9	theme	fucoidan	648:655	arg1	samples					657:663	all five fucoidan samples	639:663	all five fucoidan samples	639:663	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	2	10	theme	fibril	611:616	arg1	morphology					618:627	fibril morphology	611:627	fibril morphology	611:627	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	5	11	theme	seaweed	934:940	arg1	sources					942:948	different seaweed sources	924:948	different seaweed sources	924:948	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	5	12	with	Fucoidan	910:917	arg1	compositions					976:987	varying chemical compositions	959:987	varying chemical compositions	959:987	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	1	13	from	cytotoxicity	364:375	arg1	cells					405:409	neuronal PC-12 cells	390:409	neuronal PC-12 cells	390:409	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	3	14	theme	Aβ1-42	698:703	arg1	cytotoxicity					677:688	the cytotoxicity	673:688	the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells	673:749	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	2	15	dep	fucoidans	417:425	arg1	S					427:427	S	427:427	S	427:427	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	15	dep	fucoidans	417:425	arg1	fucoidans					417:425	Only fucoidans S, UE, and UF	412:439	Only fucoidans S, UE, and UF	412:439	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	15	dep	fucoidans	417:425	arg1	UE					430:431	UE	430:431	UE	430:431	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	2	15	dep	fucoidans	417:425	arg1	UF					438:439	UF	438:439	UF	438:439	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	1	16	theme	samples	191:197	arg1	activities					153:162	the neuroprotective activities	133:162	the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF)	133:318	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	17	with	activities	153:162	arg1	compositions					223:234	different chemical compositions	204:234	different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF)	204:318	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	4	18	dep	samples	826:832	arg1	samples					826:832	Three fucoidan samples	811:832	Three fucoidan samples (FF, UE and UF)	811:848	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	18	dep	samples	826:832	arg1	UF					846:847	UF	846:847	UF	846:847	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	18	dep	samples	826:832	arg1	UE					839:840	UE	839:840	UE	839:840	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	18	dep	samples	826:832	arg1	FF					835:836	FF	835:836	FF	835:836	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	1	19	from	aggregation	349:359	arg1	cells					405:409	neuronal PC-12 cells	390:409	neuronal PC-12 cells	390:409	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	0	20	from	activities	37:46	arg1	Undaria					88:94	Undaria	88:94	Undaria	88:94	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	0	20	from	activities	37:46	arg1	vesiculosus					72:82	Fucus vesiculosus	66:82	Fucus vesiculosus	66:82	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	1	21	theme	different	204:212	arg1	compositions					223:234	different chemical compositions	204:234	different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF)	204:318	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	0	22	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.	0:107	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	3	23	theme	peroxide	718:725	arg1	cytotoxicity					677:688	the cytotoxicity	673:688	the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells	673:749	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	4	24	theme	fucoidan	817:824	arg1	samples					826:832	Three fucoidan samples	811:832	Three fucoidan samples (FF, UE and UF)	811:848	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	24	theme	fucoidan	817:824	arg1	UF					846:847	UF	846:847	UF	846:847	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	24	theme	fucoidan	817:824	arg1	UE					839:840	UE	839:840	UE	839:840	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	4	24	theme	fucoidan	817:824	arg1	FF					835:836	FF	835:836	FF	835:836	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	1	25	theme	chemical	214:221	arg1	compositions					223:234	different chemical compositions	204:234	different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF)	204:318	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	0	26	theme	neuroprotective	21:35	arg1	activities					37:46	neuroprotective activities	21:46	neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida	21:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	5	27	theme	neuroprotective	1012:1026	arg1	activities					1028:1037	neuroprotective activities	1012:1037	neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease	1012:1114	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	5	28	contain	have	1048:1051	arg2	potential					1053:1061	potential	1053:1061	potential	1053:1061	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	5	28	contain	have	1048:1051	arg1	activities					1028:1037	neuroprotective activities	1012:1037	neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease	1012:1114	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	0	29	from	Undaria	88:94	arg1	fucoidans					51:59	fucoidans	51:59	fucoidans from Fucus vesiculosus and Undaria pinnatifida	51:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	0	29	from	Undaria	88:94	arg1	activities					37:46	neuroprotective activities	21:46	neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida	21:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	3	30	theme	hydrogen	709:716	arg1	peroxide					718:725	hydrogen peroxide	709:725	hydrogen peroxide	709:725	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	4	31	theme	neurite	891:897	arg1	outgrowth					899:907	neurite outgrowth	891:907	neurite outgrowth	891:907	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	1	32	theme	Aβ1-42	380:385	arg1	cytotoxicity					364:375	cytotoxicity	364:375	cytotoxicity	364:375	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	32	theme	Aβ1-42	380:385	arg1	aggregation					349:359	aggregation	349:359	aggregation	349:359	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	2	33	theme	fibrillisation	541:554	arg1	kinetics					556:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	0	34	theme	fucoidans	51:59	arg1	activities					37:46	neuroprotective activities	21:46	neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida	21:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	3	35	theme	neuronal	730:737	arg1	cells					745:749	neuronal PC-12 cells	730:749	neuronal PC-12 cells	730:749	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	2	36	theme	fluorometric	528:539	arg1	kinetics					556:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Thioflavin T (ThT) fluorometric fibrillisation kinetics	509:563	Only fucoidans S, UE, and UF showed anti-aggregation effects against Aβ1-42, as determined using Thioflavin T (ThT) fluorometric fibrillisation kinetics and transmission electron microscopy (TEM) of fibril morphology.
30401646	1	37	theme	neuronal	390:397	arg1	cells					405:409	neuronal PC-12 cells	390:409	neuronal PC-12 cells	390:409	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	5	38	theme	activities	1028:1037	arg1	range					1003:1007	a range	1001:1007	a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease	1001:1114	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	0	39	from	vesiculosus	72:82	arg1	fucoidans					51:59	fucoidans	51:59	fucoidans from Fucus vesiculosus and Undaria pinnatifida	51:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	0	39	from	vesiculosus	72:82	arg1	activities					37:46	neuroprotective activities	21:46	neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida	21:106	Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida.
30401646	3	40	theme	PC-12	739:743	arg1	cells					745:749	neuronal PC-12 cells	730:749	neuronal PC-12 cells	730:749	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	4	41	theme	significant	857:867	arg1	activity					869:876	significant activity	857:876	significant activity	857:876	Three fucoidan samples (FF, UE and UF) showed significant activity in enhancing neurite outgrowth.
30401646	1	42	theme	PC-12	399:403	arg1	cells					405:409	neuronal PC-12 cells	390:409	neuronal PC-12 cells	390:409	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	43	theme	neuroprotective	137:151	arg1	activities					153:162	the neuroprotective activities	133:162	the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF)	133:318	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	5	44	theme	varying	959:965	arg1	compositions					976:987	varying chemical compositions	959:987	varying chemical compositions	959:987	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	1	45	dep	vesiculosus	256:266	arg1	FE					269:270	FE	269:270	FE	269:270	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	45	dep	vesiculosus	256:266	arg1	UE					309:310	UE	309:310	UE	309:310	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	45	dep	vesiculosus	256:266	arg1	S					281:281	S	281:281	S	281:281	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	45	dep	vesiculosus	256:266	arg1	FF					273:274	FF	273:274	FF	273:274	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	1	45	dep	vesiculosus	256:266	arg1	UF					316:317	UF	316:317	UF	316:317	This study investigated the neuroprotective activities of five different fucoidan samples with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ1-42 in neuronal PC-12 cells.
30401646	3	46	theme	apoptosis	782:790	arg1	inhibition					768:777	inhibition	768:777	inhibition of apoptosis induced by Aβ1-42	768:808	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	5	47	theme	chemical	967:974	arg1	compositions					976:987	varying chemical compositions	959:987	varying chemical compositions	959:987	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30401646	3	48	from	cytotoxicity	677:688	arg1	cells					745:749	neuronal PC-12 cells	730:749	neuronal PC-12 cells	730:749	However, all five fucoidan samples reduced the cytotoxicity of both Aβ1-42 and hydrogen peroxide in neuronal PC-12 cells and demonstrated inhibition of apoptosis induced by Aβ1-42.
30401646	5	49	from	sources	942:948	arg1	Fucoidan					910:917	Fucoidan	910:917	Fucoidan from different seaweed sources and with varying chemical compositions	910:987	Fucoidan from different seaweed sources and with varying chemical compositions demonstrate a range of neuroprotective activities that may have potential to alter Aβ1-42 neurotoxicity in Alzheimer's disease.
30372664	10	0	theme	sites	1493:1497	arg1	dispersion					1456:1465	a better dispersion	1447:1465	a better dispersion of overall the adsorption sites	1447:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	10	0	theme	sites	1493:1497	arg1	constriction					1409:1420	reduced constriction	1401:1420	reduced constriction of the zeolite pores	1401:1441	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	10	1	with	material	1352:1359	arg1	content					1386:1392	the lowest chitosan content	1366:1392	the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites	1366:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	10	2	theme	reduced	1401:1407	arg1	constriction					1409:1420	reduced constriction	1401:1420	reduced constriction of the zeolite pores	1401:1441	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	7	3	theme	potentiometric	893:906	arg1	techniques					918:927	potentiometric titration techniques	893:927	potentiometric titration techniques	893:927	The composites were characterized by Electron Microscopy, FT-IR, N2 physisorption, and potentiometric titration techniques.
30372664	1	4	theme	found	138:142	arg1	pollutants					160:169	the most often found, and resilient, pollutants	123:169	the most often found, and resilient, pollutants threatening the health of millions of people around the globe	123:231	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	0	5	theme	aqueous	71:77	arg1	solution					79:86	aqueous solution	71:86	aqueous solution	71:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	0	6	from	effect	12:17	arg1	solution					79:86	aqueous solution	71:86	aqueous solution	71:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	0	6	from	effect	12:17	arg1	removal					46:52	the removal	42:52	the removal of fluoride from aqueous solution	42:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	2	7	theme	fluorine-ions	316:328	arg1	removal					305:311	the removal	301:311	the removal of fluorine-ions	301:328	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	10	8	theme	chitosan	1377:1384	arg1	content					1386:1392	the lowest chitosan content	1366:1392	the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites	1366:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	6	9	from	solution	796:803	arg1	removal					763:769	the removal	759:769	the removal of fluoride from aqueous solution	759:803	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	3	10	dep	biopolymer	353:362	arg1	restricts					458:466	restricts	458:466	restricts its application	458:482	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	9	11	theme	chemical	1116:1123	arg1	interaction					1125:1135	a chemical interaction	1114:1135	a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors	1114:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	9	12	dep	zeolite	1149:1155	arg1	components					1170:1179	components	1170:1179	components	1170:1179	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	9	13	theme	simple	1246:1251	arg1	mixture					1262:1268	a simple physical mixture	1244:1268	a simple physical mixture of the precursors	1244:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	5	14	theme	zeolite-to-chitosan	608:626	arg1	ration					628:633	A proper zeolite-to-chitosan ration	599:633	A proper zeolite-to-chitosan ration	599:633	A proper zeolite-to-chitosan ration must be kept to prevent a collapse of the material's capacity.
30372664	6	15	theme	1:3	728:730	arg1	ratios					710:715	ratios	710:715	ratios of 1:1 and 1:3	710:730	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	2	16	theme	biosorbents	245:255	arg1	use					238:240	The use	234:240	The use of biosorbents	234:255	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	2	16	theme	biosorbents	245:255	arg1	technique					287:295	an interesting alternative technique	260:295	an interesting alternative technique for the removal of fluorine-ions	260:328	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	9	17	theme	physical	1253:1260	arg1	mixture					1262:1268	a simple physical mixture	1244:1268	a simple physical mixture of the precursors	1244:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	8	18	theme	pH	981:982	arg1	effects					970:976	the effects	966:976	the effects of pH and temperature	966:998	During fluoride adsorption studies, the effects of pH and temperature were analysed and thermodynamic parameters for adsorption were calculated.
30372664	7	19	theme	N2	871:872	arg1	physisorption					874:886	N2 physisorption	871:886	N2 physisorption	871:886	The composites were characterized by Electron Microscopy, FT-IR, N2 physisorption, and potentiometric titration techniques.
30372664	12	20	theme	functional	1674:1683	arg1	biocomposites					1685:1697	functional biocomposites	1674:1697	functional biocomposites for the removal of pollutants from aqueous solutions	1674:1750	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	6	21	theme	1:1	720:722	arg1	ratios					710:715	ratios	710:715	ratios of 1:1 and 1:3	710:730	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	10	22	theme	adsorption	1297:1306	arg1	capacities					1308:1317	Maximum adsorption capacities	1289:1317	Maximum adsorption capacities	1289:1317	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	1	23	theme	resilient	149:157	arg1	pollutants					160:169	the most often found, and resilient, pollutants	123:169	the most often found, and resilient, pollutants threatening the health of millions of people around the globe	123:231	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	6	24	theme	aqueous	788:794	arg1	solution					796:803	aqueous solution	788:803	aqueous solution	788:803	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	8	25	theme	temperature	988:998	arg1	effects					970:976	the effects	966:976	the effects of pH and temperature	966:998	During fluoride adsorption studies, the effects of pH and temperature were analysed and thermodynamic parameters for adsorption were calculated.
30372664	10	26	theme	lowest	1370:1375	arg1	content					1386:1392	the lowest chitosan content	1366:1392	the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites	1366:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	0	27	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution	0:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	7	28	theme	Electron	843:850	arg1	Microscopy					852:861	Electron Microscopy	843:861	Electron Microscopy	843:861	The composites were characterized by Electron Microscopy, FT-IR, N2 physisorption, and potentiometric titration techniques.
30372664	2	29	theme	alternative	275:285	arg1	use					238:240	The use	234:240	The use of biosorbents	234:255	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	2	29	theme	alternative	275:285	arg1	technique					287:295	an interesting alternative technique	260:295	an interesting alternative technique for the removal of fluorine-ions	260:328	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	1	30	theme	pollutants	160:169	arg1	pollutants					160:169	the most often found, and resilient, pollutants	123:169	the most often found, and resilient, pollutants threatening the health of millions of people around the globe	123:231	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	1	30	theme	pollutants	160:169	arg1	one					116:118	one	116:118	one	116:118	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	0	31	theme	zeolite/chitosan	22:37	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution	0:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	8	32	theme	adsorption	946:955	arg1	studies					957:963	fluoride adsorption studies	937:963	fluoride adsorption studies	937:963	During fluoride adsorption studies, the effects of pH and temperature were analysed and thermodynamic parameters for adsorption were calculated.
30372664	10	33	theme	due	1394:1396	arg1	content					1386:1392	the lowest chitosan content	1366:1392	the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites	1366:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	8	34	theme	fluoride	937:944	arg1	studies					957:963	fluoride adsorption studies	937:963	fluoride adsorption studies	937:963	During fluoride adsorption studies, the effects of pH and temperature were analysed and thermodynamic parameters for adsorption were calculated.
30372664	3	35	with	biopolymer	353:362	arg1	groups					377:382	surface groups	369:382	surface groups	369:382	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	6	36	from	ratios	710:715	arg1	ZCCs					702:705	Two ZCCs	698:705	Two ZCCs at ratios of 1:1 and 1:3	698:730	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	3	37	theme	natural	345:351	arg1	biopolymer					353:362	a natural biopolymer	343:362	a natural biopolymer with surface groups capable of removing fluorine	343:411	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	3	37	theme	natural	345:351	arg1	Chitosan					331:338	Chitosan	331:338	Chitosan	331:338	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	12	38	theme	aqueous	1734:1740	arg1	solutions					1742:1750	aqueous solutions	1734:1750	aqueous solutions	1734:1750	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	11	39	contain	had	1524:1526	arg2	impact					1557:1562	a significant, and negative, impact	1528:1562	a significant, and negative, impact	1528:1562	Both pH and temperature had a significant, and negative, impact on the adsorption; these effects were discussed.
30372664	11	39	contain	had	1524:1526	arg1	temperature					1512:1522	temperature	1512:1522	temperature	1512:1522	Both pH and temperature had a significant, and negative, impact on the adsorption; these effects were discussed.
30372664	11	39	contain	had	1524:1526	arg1	pH					1505:1506	pH	1505:1506	pH	1505:1506	Both pH and temperature had a significant, and negative, impact on the adsorption; these effects were discussed.
30372664	0	40	from	solution	79:86	arg1	removal					46:52	the removal	42:52	the removal of fluoride from aqueous solution	42:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	0	40	from	solution	79:86	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution	0:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	12	41	from	solutions	1742:1750	arg1	removal					1707:1713	the removal	1703:1713	the removal of pollutants from aqueous solutions	1703:1750	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	3	42	theme	capable	384:390	arg1	biopolymer					353:362	a natural biopolymer	343:362	a natural biopolymer with surface groups capable of removing fluorine	343:411	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	3	42	theme	capable	384:390	arg1	Chitosan					331:338	Chitosan	331:338	Chitosan	331:338	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	4	43	theme	such	523:526	arg1	limitations					528:538	such limitations	523:538	such limitations	523:538	In the present work, we proposed that such limitations can be overcame by forming a composite with zeolite (ZCC).
30372664	8	44	theme	thermodynamic	1018:1030	arg1	parameters					1032:1041	thermodynamic parameters	1018:1041	thermodynamic parameters for adsorption	1018:1056	During fluoride adsorption studies, the effects of pH and temperature were analysed and thermodynamic parameters for adsorption were calculated.
30372664	6	45	theme	fluoride	774:781	arg1	removal					763:769	the removal	759:769	the removal of fluoride from aqueous solution	759:803	Two ZCCs at ratios of 1:1 and 1:3 were formed and tested for the removal of fluoride from aqueous solution.
30372664	12	46	dep	an	1641:1642	arg1	advance					1644:1650	advance	1644:1650	advance	1644:1650	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	10	47	theme	pores	1437:1441	arg1	dispersion					1456:1465	a better dispersion	1447:1465	a better dispersion of overall the adsorption sites	1447:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	10	47	theme	pores	1437:1441	arg1	constriction					1409:1420	reduced constriction	1401:1420	reduced constriction of the zeolite pores	1401:1441	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	4	48	with	composite	569:577	arg1	ZCC					593:595	ZCC	593:595	ZCC	593:595	In the present work, we proposed that such limitations can be overcame by forming a composite with zeolite (ZCC).
30372664	4	48	with	composite	569:577	arg1	zeolite					584:590	zeolite	584:590	zeolite	584:590	In the present work, we proposed that such limitations can be overcame by forming a composite with zeolite (ZCC).
30372664	10	49	theme	Maximum	1289:1295	arg1	capacities					1308:1317	Maximum adsorption capacities	1289:1317	Maximum adsorption capacities	1289:1317	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	3	50	theme	surface	369:375	arg1	groups					377:382	surface groups	369:382	surface groups	369:382	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	4	51	theme	present	492:498	arg1	work					500:503	the present work	488:503	the present work	488:503	In the present work, we proposed that such limitations can be overcame by forming a composite with zeolite (ZCC).
30372664	9	52	theme	precursors	1277:1286	arg1	mixture					1262:1268	a simple physical mixture	1244:1268	a simple physical mixture of the precursors	1244:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	10	53	theme	better	1449:1454	arg1	dispersion					1456:1465	a better dispersion	1447:1465	a better dispersion of overall the adsorption sites	1447:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	9	54	theme	superior	1194:1201	arg1	performance					1214:1224	a superior adsorption performance	1192:1224	a superior adsorption performance than if there was a simple physical mixture of the precursors	1192:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	5	55	theme	proper	601:606	arg1	ration					628:633	A proper zeolite-to-chitosan ration	599:633	A proper zeolite-to-chitosan ration	599:633	A proper zeolite-to-chitosan ration must be kept to prevent a collapse of the material's capacity.
30372664	10	56	theme	zeolite	1429:1435	arg1	pores					1437:1441	the zeolite pores	1425:1441	the zeolite pores	1425:1441	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	9	57	theme	adsorption	1203:1212	arg1	performance					1214:1224	a superior adsorption performance	1192:1224	a superior adsorption performance than if there was a simple physical mixture of the precursors	1192:1286	The results demonstrated that there is a chemical interaction between the zeolite and chitosan components leading to a superior adsorption performance than if there was a simple physical mixture of the precursors.
30372664	10	58	theme	composite	1342:1350	arg1	material					1352:1359	the composite material	1338:1359	the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites	1338:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	3	59	theme	mechanical	437:446	arg1	stability					448:456	mechanical stability	437:456	mechanical stability	437:456	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	2	60	theme	interesting	263:273	arg1	use					238:240	The use	234:240	The use of biosorbents	234:255	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	2	60	theme	interesting	263:273	arg1	technique					287:295	an interesting alternative technique	260:295	an interesting alternative technique for the removal of fluorine-ions	260:328	The use of biosorbents is an interesting alternative technique for the removal of fluorine-ions.
30372664	1	61	theme	millions	197:204	arg1	health					187:192	the health	183:192	the health of millions of people around the globe	183:231	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	11	62	theme	significant	1530:1540	arg1	impact					1557:1562	a significant, and negative, impact	1528:1562	a significant, and negative, impact	1528:1562	Both pH and temperature had a significant, and negative, impact on the adsorption; these effects were discussed.
30372664	7	63	theme	titration	908:916	arg1	techniques					918:927	potentiometric titration techniques	893:927	potentiometric titration techniques	893:927	The composites were characterized by Electron Microscopy, FT-IR, N2 physisorption, and potentiometric titration techniques.
30372664	3	64	theme	stability	448:456	arg1	lack					429:432	their lack	423:432	their lack of mechanical stability	423:456	Chitosan is a natural biopolymer with surface groups capable of removing fluorine; however, their lack of mechanical stability restricts its application.
30372664	5	65	theme	capacity	688:695	arg1	collapse					661:668	a collapse	659:668	a collapse of the material's capacity	659:695	A proper zeolite-to-chitosan ration must be kept to prevent a collapse of the material's capacity.
30372664	12	66	theme	pollutants	1718:1727	arg1	removal					1707:1713	the removal	1703:1713	the removal of pollutants from aqueous solutions	1703:1750	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	10	67	theme	adsorption	1482:1491	arg1	sites					1493:1497	overall the adsorption sites	1470:1497	overall the adsorption sites	1470:1497	Maximum adsorption capacities were reached using the composite material with the lowest chitosan content due to reduced constriction of the zeolite pores and a better dispersion of overall the adsorption sites.
30372664	12	68	theme	biocomposites	1685:1697	arg1	development					1659:1669	the development	1655:1669	the development of functional biocomposites for the removal of pollutants from aqueous solutions	1655:1750	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
30372664	1	69	theme	people	209:214	arg1	millions					197:204	millions	197:204	millions of people around the globe	197:231	Today, fluoride represents one of the most often found, and resilient, pollutants threatening the health of millions of people around the globe.
30372664	0	70	theme	fluoride	57:64	arg1	removal					46:52	the removal	42:52	the removal of fluoride from aqueous solution	42:86	Synergistic effect of zeolite/chitosan in the removal of fluoride from aqueous solution.
30372664	11	71	theme	negative	1547:1554	arg1	impact					1557:1562	a significant, and negative, impact	1528:1562	a significant, and negative, impact	1528:1562	Both pH and temperature had a significant, and negative, impact on the adsorption; these effects were discussed.
30372664	12	72	theme	present	1617:1623	arg1	work					1625:1628	The present work	1613:1628	The present work	1613:1628	The present work represents an advance in the development of functional biocomposites for the removal of pollutants from aqueous solutions.
31089892	0	0	theme	Inhalable	95:103	arg1	Pharmaceutics					105:117	Inhalable Pharmaceutics	95:117	Inhalable Pharmaceutics	95:117	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	8	1	theme	systems	1604:1610	arg1	azeotrope					1586:1594	the azeotrope	1582:1594	the azeotrope of such systems during distillation	1582:1630	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	5	2	dep	RESULTS	915:921	arg1	used					949:952	used	949:952	are used to explain the different particle morphologies dried from solutions at different co-solvent compositions	945:1057	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	0	3	with	Modeling	40:47	arg1	Pharmaceutics					105:117	Inhalable Pharmaceutics	95:117	Inhalable Pharmaceutics	95:117	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	3	4	theme	internal	557:564	arg1	transfers					573:581	internal solute transfers	557:581	internal solute transfers	557:581	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	6	5	theme	particle	1171:1178	arg1	morphology					1149:1158	the general morphology	1137:1158	the general morphology of a dried particle	1137:1178	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	9	6	theme	iso-compositional	1742:1758	arg1	ratio					1760:1764	the iso-compositional ratio	1738:1764	the iso-compositional ratio	1738:1764	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	1	7	theme	solid	207:211	arg1	excipients					213:222	solid excipients	207:222	solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery	207:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	2	8	theme	Various	314:320	arg1	water					322:326	Various water and ethanol co-solvent systems	314:357	water	322:326	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	9	9	theme	such	1815:1818	arg1	systems					1820:1826	such systems	1815:1826	such systems	1815:1826	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	4	10	theme	dried	835:839	arg1	microparticles					841:854	dried microparticles	835:854	dried microparticles for further analytical investigations and ultramicroscopy	835:912	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	4	11	theme	chain	813:817	arg1	setup					819:823	a monodisperse droplet chain setup	790:823	a monodisperse droplet chain setup	790:823	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	7	12	theme	product	1307:1313	arg1	development					1315:1325	product development	1307:1325	product development	1307:1325	This fact complicates product development for such systems.
31089892	9	13	theme	systems	1820:1826	arg1	design					1781:1786	design	1781:1786	design	1781:1786	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	9	13	theme	systems	1820:1826	arg1	process					1792:1798	process	1792:1798	process	1792:1798	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	2	14	theme	co-solvent	340:349	arg1	systems					351:357	Various water and ethanol co-solvent systems	314:357	systems	351:357	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	3	15	from	setups	668:673	arg1	results					629:635	results	629:635	results from two separate experimental setups	629:673	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	3	16	theme	evaporation	535:545	arg1	rates					547:551	the droplet evaporation rates	523:551	the droplet evaporation rates	523:551	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	1	17	contain	containing	196:205	arg1	microdroplets					182:194	multi-solvent microdroplets	168:194	multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery	168:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	1	17	contain	containing	196:205	arg2	excipients					213:222	solid excipients	207:222	solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery	207:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	1	18	from	microdroplets	182:194	arg1	formation					153:161	particle formation	144:161	particle formation	144:161	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	1	18	from	microdroplets	182:194	arg1	Evaporation					128:138	PURPOSE Evaporation	120:138	PURPOSE Evaporation	120:138	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	2	19	theme	ethanol	332:338	arg1	systems					351:357	Various water and ethanol co-solvent systems	314:357	systems	351:357	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	5	20	theme	different	1025:1033	arg1	compositions					1046:1057	different co-solvent compositions	1025:1057	different co-solvent compositions	1025:1057	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	6	21	theme	Péclet	1192:1197	arg1	number					1199:1204	the Péclet number	1188:1204	the Péclet number	1188:1204	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	6	21	theme	Péclet	1192:1197	arg1	parameters					1114:1123	the two parameters	1106:1123	the two parameters controlling the general morphology of a dried particle	1106:1178	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	8	22	theme	iso-compositional	1415:1431	arg1	point					1433:1437	the iso-compositional point	1411:1437	the iso-compositional point	1411:1437	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	6	23	theme	saturation	1224:1233	arg1	number					1199:1204	the Péclet number	1188:1204	the Péclet number	1188:1204	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	6	23	theme	saturation	1224:1233	arg1	degree					1214:1219	the degree	1210:1219	the degree of saturation	1210:1233	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	6	23	theme	saturation	1224:1233	arg1	parameters					1114:1123	the two parameters	1106:1123	the two parameters controlling the general morphology of a dried particle	1106:1178	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	6	24	theme	general	1141:1147	arg1	morphology					1149:1158	the general morphology	1137:1158	the general morphology of a dried particle	1137:1178	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	4	25	theme	further	860:866	arg1	investigations					879:892	further analytical investigations	860:892	further analytical investigations	860:892	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	0	26	theme	Microdroplet	14:25	arg1	Evaporation					27:37	Multi-Solvent Microdroplet Evaporation	0:37	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.	0:118	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	6	27	theme	numerical	1073:1081	arg1	data					1083:1086	The obtained numerical data	1060:1086	The obtained numerical data	1060:1086	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	7	28	theme	such	1331:1334	arg1	systems					1336:1342	such systems	1331:1342	such systems	1331:1342	This fact complicates product development for such systems.
31089892	4	29	theme	droplet	805:811	arg1	setup					819:823	a monodisperse droplet chain setup	790:823	a monodisperse droplet chain setup	790:823	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	3	30	theme	METHODS	476:482	arg1	methods					494:500	METHODS Numerical methods	476:500	METHODS Numerical methods	476:500	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	0	31	theme	Multi-Solvent	0:12	arg1	Evaporation					27:37	Multi-Solvent Microdroplet Evaporation	0:37	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.	0:118	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	2	32	dep	actives	383:389	arg1	leucine					435:441	leucine	435:441	leucine	435:441	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	32	dep	actives	383:389	arg1	trehalose					448:456	trehalose	448:456	trehalose	448:456	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	32	dep	actives	383:389	arg1	budesonide					423:432	budesonide	423:432	budesonide	423:432	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	32	dep	actives	383:389	arg1	actives					383:389	actives	383:389	actives	383:389	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	32	dep	actives	383:389	arg1	beclomethasone					407:420	beclomethasone	407:420	beclomethasone	407:420	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	8	33	theme	droplet	1527:1533	arg1	ratios					1470:1475	the solvent ratios	1458:1475	the solvent ratios	1458:1475	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	8	33	theme	droplet	1527:1533	arg1	properties					1509:1518	other composition-dependent properties	1481:1518	other composition-dependent properties	1481:1518	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	3	34	theme	Numerical	484:492	arg1	methods					494:500	METHODS Numerical methods	476:500	METHODS Numerical methods	476:500	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	4	35	theme	monodisperse	792:803	arg1	setup					819:823	a monodisperse droplet chain setup	790:823	a monodisperse droplet chain setup	790:823	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	5	36	theme	co-solvent	1035:1044	arg1	compositions					1046:1057	different co-solvent compositions	1025:1057	different co-solvent compositions	1025:1057	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	1	37	theme	PURPOSE	120:126	arg1	Evaporation					128:138	PURPOSE Evaporation	120:138	PURPOSE Evaporation	120:138	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	5	38	theme	numerical	927:935	arg1	results					937:943	The numerical results	923:943	The numerical results	923:943	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	3	39	used	used	507:510	arg2	methods					494:500	METHODS Numerical methods	476:500	METHODS Numerical methods	476:500	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	6	40	theme	multi-solvent	1262:1274	arg1	droplet					1276:1282	a multi-solvent droplet	1260:1282	a multi-solvent droplet	1260:1282	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	8	41	theme	composition-dependent	1487:1507	arg1	properties					1509:1518	other composition-dependent properties	1481:1518	other composition-dependent properties	1481:1518	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	3	42	theme	solute	566:571	arg1	transfers					573:581	internal solute transfers	557:581	internal solute transfers	557:581	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	9	43	theme	experimental	1659:1670	arg1	CONCLUSIONS					1633:1643	CONCLUSIONS	1633:1643	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems	1633:1704	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	1	44	theme	therapeutic	254:264	arg1	agents					266:271	therapeutic agents	254:271	therapeutic agents intended for lung delivery	254:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	2	45	theme	excipients	395:404	arg1	trehalose					448:456	trehalose	448:456	trehalose	448:456	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	variety					372:378	a variety	370:378	a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose)	370:457	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	beclomethasone					407:420	beclomethasone	407:420	beclomethasone	407:420	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	budesonide					423:432	budesonide	423:432	budesonide	423:432	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	actives					383:389	actives	383:389	actives	383:389	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	excipients					395:404	excipients	395:404	excipients	395:404	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	45	theme	excipients	395:404	arg1	leucine					435:441	leucine	435:441	leucine	435:441	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	3	46	theme	separate	646:653	arg1	setups					668:673	two separate experimental setups	642:673	two separate experimental setups	642:673	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	5	47	theme	different	969:977	arg1	morphologies					988:999	the different particle morphologies	965:999	the different particle morphologies dried from solutions at different co-solvent compositions	965:1057	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	1	48	theme	agents	266:271	arg1	spray-drying					238:249	spray-drying	238:249	spray-drying of therapeutic agents intended for lung delivery	238:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	4	49	theme	evaporation	741:751	arg1	rates					753:757	the evaporation rates	737:757	the evaporation rates of multicomponent droplets	737:784	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	2	50	contain	containing	359:368	arg2	actives					383:389	actives	383:389	actives	383:389	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	excipients					395:404	excipients	395:404	excipients	395:404	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	leucine					435:441	leucine	435:441	leucine	435:441	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	variety					372:378	a variety	370:378	a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose)	370:457	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	trehalose					448:456	trehalose	448:456	trehalose	448:456	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	beclomethasone					407:420	beclomethasone	407:420	beclomethasone	407:420	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg2	budesonide					423:432	budesonide	423:432	budesonide	423:432	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	50	contain	containing	359:368	arg1	water					322:326	Various water and ethanol co-solvent systems	314:357	water	322:326	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	3	51	theme	experimental	655:666	arg1	setups					668:673	two separate experimental setups	642:673	two separate experimental setups	642:673	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	6	52	theme	dried	1165:1169	arg1	particle					1171:1178	a dried particle	1163:1178	a dried particle	1163:1178	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	4	53	theme	electrodynamic	694:707	arg1	balance					709:715	an electrodynamic balance	691:715	an electrodynamic balance	691:715	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	5	54	theme	particle	979:986	arg1	morphologies					988:999	the different particle morphologies	965:999	the different particle morphologies dried from solutions at different co-solvent compositions	965:1057	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	8	55	theme	similar	1571:1577	arg1	evaporation					1558:1568	evaporation	1558:1568	evaporation	1558:1568	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	8	56	theme	solvent	1462:1468	arg1	ratios					1470:1475	the solvent ratios	1458:1475	the solvent ratios	1458:1475	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	4	57	theme	droplets	777:784	arg1	rates					753:757	the evaporation rates	737:757	the evaporation rates of multicomponent droplets	737:784	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	1	58	theme	particle	144:151	arg1	formation					153:161	particle formation	144:161	particle formation	144:161	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	9	59	dep	CONCLUSIONS	1633:1643	arg1	analysis					1672:1679	analysis	1672:1679	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems	1633:1704	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	6	60	theme	obtained	1064:1071	arg1	data					1083:1086	The obtained numerical data	1060:1086	The obtained numerical data	1060:1086	The obtained numerical data clearly show that the two parameters controlling the general morphology of a dried particle, namely the Péclet number and the degree of saturation, can change with time in a multi-solvent droplet.
31089892	4	61	theme	analytical	868:877	arg1	investigations					879:892	further analytical investigations	860:892	further analytical investigations	860:892	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	4	62	used	used	721:724	arg2	balance					709:715	an electrodynamic balance	691:715	an electrodynamic balance	691:715	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31089892	3	63	theme	droplet	527:533	arg1	rates					547:551	the droplet evaporation rates	523:551	the droplet evaporation rates	523:551	METHODS Numerical methods were used to predict the droplet evaporation rates and internal solute transfers, and their results verified and compared with results from two separate experimental setups.
31089892	8	64	theme	other	1481:1485	arg1	properties					1509:1518	other composition-dependent properties	1481:1518	other composition-dependent properties	1481:1518	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	5	65	used	used	949:952	arg2	results					937:943	The numerical results	923:943	The numerical results	923:943	RESULTS The numerical results are used to explain the different particle morphologies dried from solutions at different co-solvent compositions.
31089892	9	66	theme	systems	1698:1704	arg1	analysis					1672:1679	analysis	1672:1679	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems	1633:1704	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	8	67	theme	such	1599:1602	arg1	systems					1604:1610	such systems	1599:1610	such systems	1599:1610	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	0	68	dep	Evaporation	27:37	arg1	Measurement					53:63	Measurement	53:63	Measurement	53:63	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	0	68	dep	Evaporation	27:37	arg1	Modeling					40:47	Modeling	40:47	Modeling	40:47	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	2	69	theme	actives	383:389	arg1	trehalose					448:456	trehalose	448:456	trehalose	448:456	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	variety					372:378	a variety	370:378	a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose)	370:457	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	beclomethasone					407:420	beclomethasone	407:420	beclomethasone	407:420	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	budesonide					423:432	budesonide	423:432	budesonide	423:432	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	actives					383:389	actives	383:389	actives	383:389	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	excipients					395:404	excipients	395:404	excipients	395:404	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	2	69	theme	actives	383:389	arg1	leucine					435:441	leucine	435:441	leucine	435:441	Various water and ethanol co-solvent systems containing a variety of actives and excipients (beclomethasone, budesonide, leucine, and trehalose) were considered.
31089892	1	70	theme	lung	286:289	arg1	delivery					291:298	lung delivery	286:298	lung delivery	286:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	0	71	theme	Kinetics	81:88	arg1	Measurement					53:63	Measurement	53:63	Measurement	53:63	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	0	71	theme	Kinetics	81:88	arg1	Modeling					40:47	Modeling	40:47	Modeling	40:47	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	9	72	dep	design	1781:1786	arg1	development					1800:1810	development	1800:1810	development	1800:1810	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	9	72	dep	design	1781:1786	arg1	the					1777:1779	the	1777:1779	the	1777:1779	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	0	73	with	Measurement	53:63	arg1	Pharmaceutics					105:117	Inhalable Pharmaceutics	95:117	Inhalable Pharmaceutics	95:117	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	1	74	theme	multi-solvent	168:180	arg1	microdroplets					182:194	multi-solvent microdroplets	168:194	multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery	168:298	PURPOSE Evaporation and particle formation from multi-solvent microdroplets containing solid excipients pertaining to spray-drying of therapeutic agents intended for lung delivery were studied.
31089892	8	75	theme	additional	1359:1368	arg1	complexity					1370:1379	this additional complexity	1354:1379	this additional complexity	1354:1379	However, this additional complexity vanishes at what we define as the iso-compositional point, which occurs when the solvent ratios and other composition-dependent properties of the droplet remain constant during evaporation, similar to the azeotrope of such systems during distillation.
31089892	0	76	theme	Spray-Drying	68:79	arg1	Kinetics					81:88	Spray-Drying Kinetics	68:88	Spray-Drying Kinetics	68:88	Multi-Solvent Microdroplet Evaporation: Modeling and Measurement of Spray-Drying Kinetics with Inhalable Pharmaceutics.
31089892	9	77	theme	multi-solvent	1684:1696	arg1	systems					1698:1704	multi-solvent systems	1684:1704	multi-solvent systems	1684:1704	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	9	78	theme	Numerical	1645:1653	arg1	CONCLUSIONS					1633:1643	CONCLUSIONS	1633:1643	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems	1633:1704	CONCLUSIONS Numerical and experimental analysis of multi-solvent systems indicate that spray-drying near the iso-compositional ratio simplifies the design and process development of such systems.
31089892	4	79	theme	multicomponent	762:775	arg1	droplets					777:784	multicomponent droplets	762:784	multicomponent droplets	762:784	In particular, an electrodynamic balance was used to measure the evaporation rates of multicomponent droplets and a monodisperse droplet chain setup collected dried microparticles for further analytical investigations and ultramicroscopy.
31138126	7	0	theme	glycoside	1434:1442	arg1	hydrolase					1444:1452	209 glycoside hydrolase	1430:1452	209 glycoside hydrolase	1430:1452	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	1	1	theme	plant	254:258	arg1	polysaccharides					270:284	plant cell wall polysaccharides	254:284	plant cell wall polysaccharides	254:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	4	2	theme	carbohydrate-active	822:840	arg1	enzymes					842:848	specific carbohydrate-active enzymes	813:848	specific carbohydrate-active enzymes (CAZymes)	813:858	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	2	theme	carbohydrate-active	822:840	arg1	CAZymes					851:857	CAZymes	851:857	CAZymes	851:857	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	6	3	theme	nuclear	1179:1185	arg1	genome					1187:1192	The PACBio sequenced nuclear genome	1158:1192	The PACBio sequenced nuclear genome of P. radiata	1158:1206	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	6	4	dep	RESULTS	1150:1156	arg1	assembled					1212:1220	assembled	1212:1220	assembled to 93 contigs with 72X sequencing coverage	1212:1263	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	6	4	dep	RESULTS	1150:1156	arg1	annotated					1269:1277	annotated	1269:1277	annotated	1269:1277	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	10	5	theme	secondary	1956:1964	arg1	metabolites					1966:1976	secondary metabolites	1956:1976	secondary metabolites	1956:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	13	6	theme	carbon	2578:2583	arg1	ecosystems					2599:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	11	7	theme	ABC	2035:2037	arg1	proteins					2039:2046	predicted ABC proteins	2025:2046	predicted ABC proteins	2025:2046	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	11	8	theme	small	2153:2157	arg1	proteins					2168:2175	small secreted proteins	2153:2175	small secreted proteins	2153:2175	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	6	9	theme	72X	1241:1243	arg1	coverage					1256:1263	72X sequencing coverage	1241:1263	72X sequencing coverage	1241:1263	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	11	10	theme	radiata	2070:2076	arg1	genome					2078:2083	P. radiata genome	2067:2083	P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins	2067:2175	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	6	11	with	genome	1298:1303	arg1	genes					1368:1372	approximately 14 082 predicted protein-coding genes	1322:1372	approximately 14 082 predicted protein-coding genes	1322:1372	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	1	12	theme	type	189:192	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	12	theme	type	189:192	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	0	13	theme	fungi	131:135	arg1	machinery					108:116	lignocellulose decomposition machinery	79:116	lignocellulose decomposition machinery of phlebioid fungi	79:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	12	14	theme	wood	2377:2380	arg1	decomposition					2382:2394	wood decomposition	2377:2394	wood decomposition	2377:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	6	15	theme	Mbp	1313:1315	arg1	genome					1298:1303	a dense genome	1290:1303	a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes	1290:1372	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	1	16	theme	various	365:371	arg1	substrates					394:403	various waste lignocellulose substrates	365:403	various waste lignocellulose substrates	365:403	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	3	17	theme	isolate	582:588	arg1	sequence					559:566	the genome sequence	548:566	the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland	548:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	1	18	theme	genus	209:213	arg1	Phlebia					215:221	the genus Phlebia	205:221	the genus Phlebia	205:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	4	19	theme	related	877:883	arg1	species					892:898	seven closely related fungal species	863:898	seven closely related fungal species	863:898	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	20	theme	wood	693:696	arg1	mechanisms					704:713	wood decay mechanisms	693:713	wood decay mechanisms	693:713	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	1	21	theme	lignocellulose	379:392	arg1	substrates					394:403	various waste lignocellulose substrates	365:403	various waste lignocellulose substrates	365:403	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	3	22	theme	P.	571:572	arg1	isolate					582:588	P. radiata isolate 79	571:591	P. radiata isolate 79 originally isolated from decayed alder wood in South Finland	571:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	9	23	theme	enzyme-encoding	1761:1775	arg1	genes					1777:1781	especially GH10 and AA9 enzyme-encoding genes	1737:1781	especially GH10 and AA9 enzyme-encoding genes according to genomic localization	1737:1815	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	12	24	theme	white	2354:2358	arg1	lifestyle					2364:2372	phlebioid white rot lifestyle	2344:2372	phlebioid white rot lifestyle in wood decomposition	2344:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	4	25	theme	mechanisms	704:713	arg1	evolution					680:688	the evolution	676:688	the evolution of wood decay mechanisms in this fungus	676:728	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	25	theme	mechanisms	704:713	arg1	clade					760:764	the Polyporales phlebioid clade	734:764	the Polyporales phlebioid clade	734:764	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	13	26	theme	fungal	2504:2509	arg1	mechanisms					2517:2526	enlightening fungal decay mechanisms	2491:2526	enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems	2491:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	6	27	theme	dense	1292:1296	arg1	genome					1298:1303	a dense genome	1290:1303	a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes	1290:1372	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	13	28	theme	forest	2592:2597	arg1	ecosystems					2599:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	6	29	theme	radiata	1200:1206	arg1	genome					1187:1192	The PACBio sequenced nuclear genome	1158:1192	The PACBio sequenced nuclear genome of P. radiata	1158:1206	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	0	30	theme	genomics	58:65	arg1	analysis					67:74	comparative genomics analysis	46:74	comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi	46:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	1	31	theme	hardwood	312:319	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	31	theme	hardwood	312:319	arg1	modifier					287:294	modifier	287:294	modifier of softwood and hardwood lignin	287:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	32	theme	rot	159:161	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	32	theme	rot	159:161	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	32	theme	rot	159:161	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	32	theme	rot	159:161	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	6	33	with	contigs	1228:1234	arg1	coverage					1256:1263	72X sequencing coverage	1241:1263	72X sequencing coverage	1241:1263	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	9	34	theme	genomic	1796:1802	arg1	localization					1804:1815	genomic localization	1796:1815	genomic localization	1796:1815	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	9	35	theme	genes	1777:1781	arg1	Clustering					1723:1732	Clustering	1723:1732	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization	1723:1815	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	1	36	from	substrates	394:403	arg1	ethanol					352:358	ethanol	352:358	ethanol from various waste lignocellulose substrates	352:403	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	5	37	theme	P.	1138:1139	arg1	radiata					1141:1147	P. radiata	1138:1147	P. radiata	1138:1147	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	4	38	from	clade	760:764	arg1	fungus					723:728	this fungus	718:728	this fungus	718:728	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	1	39	theme	Phlebia	170:176	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	39	theme	Phlebia	170:176	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	39	theme	Phlebia	170:176	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	39	theme	Phlebia	170:176	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	2	40	theme	plant	518:522	arg1	biomass					524:530	plant biomass	518:530	plant biomass	518:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	1	41	dep	BACKGROUND	138:147	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	41	dep	BACKGROUND	138:147	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	41	dep	BACKGROUND	138:147	arg1	modifier					287:294	modifier	287:294	modifier of softwood and hardwood lignin	287:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	41	dep	BACKGROUND	138:147	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	0	42	theme	decomposition	94:106	arg1	machinery					108:116	lignocellulose decomposition machinery	79:116	lignocellulose decomposition machinery of phlebioid fungi	79:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	5	43	theme	proteins reflecting	952:970	arg1	peptidases					1003:1012	the fungal lifestyle including peptidases	972:1012	proteins reflecting the fungal lifestyle including peptidases	952:1012	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	12	44	theme	oxidoreductase	2289:2302	arg1	genes					2304:2308	oxidoreductase genes	2289:2308	oxidoreductase genes	2289:2308	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	5	45	theme	fungal	976:981	arg1	peptidases					1003:1012	the fungal lifestyle including peptidases	972:1012	proteins reflecting the fungal lifestyle including peptidases	952:1012	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	0	46	theme	Genome	0:5	arg1	description					7:17	Genome description	0:17	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.	0:136	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	1	47	theme	cell	260:263	arg1	polysaccharides					270:284	plant cell wall polysaccharides	254:284	plant cell wall polysaccharides	254:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	13	48	theme	Comparative	2450:2460	arg1	genomics					2462:2469	Comparative genomics	2450:2469	Comparative genomics	2450:2469	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	2	49	theme	P.	412:413	arg1	radiata					415:421	P. radiata	412:421	P. radiata	412:421	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	2	49	theme	P.	412:413	arg1	organism					438:445	a promising organism	426:445	a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass	426:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	12	50	theme	carbohydrate	2247:2258	arg1	CAZyme					2278:2283	carbohydrate polymer attacking CAZyme	2247:2283	carbohydrate polymer attacking CAZyme	2247:2283	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	1	51	theme	polysaccharides	270:284	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	51	theme	polysaccharides	270:284	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	51	theme	polysaccharides	270:284	arg1	modifier					287:294	modifier	287:294	modifier of softwood and hardwood lignin	287:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	51	theme	polysaccharides	270:284	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	7	52	theme	enzyme-encoding	1533:1547	arg1	genes					1549:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	12	53	theme	P.	2213:2214	arg1	radiata					2216:2222	P. radiata	2213:2222	P. radiata	2213:2222	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	0	54	theme	radiata	30:36	arg1	description					7:17	Genome description	0:17	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.	0:136	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	12	55	from	lifestyle	2364:2372	arg1	decomposition					2382:2394	wood decomposition	2377:2394	wood decomposition	2377:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	7	56	theme	auxiliary	1517:1525	arg1	genes					1549:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	2	57	theme	promising	428:436	arg1	radiata					415:421	P. radiata	412:421	P. radiata	412:421	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	2	57	theme	promising	428:436	arg1	organism					438:445	a promising organism	426:445	a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass	426:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	3	58	theme	alder	626:630	arg1	wood					632:635	decayed alder wood	618:635	decayed alder wood in South Finland	618:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	13	59	theme	recalcitrant	2557:2568	arg1	ecosystems					2599:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	1	60	theme	softwood	299:306	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	60	theme	softwood	299:306	arg1	modifier					287:294	modifier	287:294	modifier of softwood and hardwood lignin	287:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	5	61	theme	secreted	1035:1042	arg1	proteins					1044:1051	small secreted proteins	1029:1051	small secreted proteins	1029:1051	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	8	62	theme	other	1588:1592	arg1	fungi					1604:1608	other phlebioid fungi	1588:1608	other phlebioid fungi	1588:1608	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	4	63	theme	gene	767:770	arg1	content					772:778	gene content	767:778	gene content	767:778	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	1	64	theme	Phlebia	215:221	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	64	theme	Phlebia	215:221	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	5	65	theme	including	993:1001	arg1	peptidases					1003:1012	the fungal lifestyle including peptidases	972:1012	proteins reflecting the fungal lifestyle including peptidases	952:1012	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	12	66	theme	further	2433:2439	arg1	studies					2441:2447	further studies	2433:2447	further studies	2433:2447	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	8	67	theme	fungi	1604:1608	arg1	genomes					1577:1583	the genomes	1573:1583	the genomes of other phlebioid fungi	1573:1608	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	12	68	theme	attacking	2268:2276	arg1	CAZyme					2278:2283	carbohydrate polymer attacking CAZyme	2247:2283	carbohydrate polymer attacking CAZyme	2247:2283	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	10	69	theme	rich	1901:1904	arg1	repertoire					1906:1915	a rich repertoire	1899:1915	a rich repertoire of genes involved in the production of secondary metabolites	1899:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	10	69	theme	rich	1901:1904	arg1	genes					1920:1924	genes	1920:1924	genes involved in the production of secondary metabolites	1920:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	7	70	theme	polysaccharide	1483:1496	arg1	lyase					1498:1502	8 polysaccharide lyase	1481:1502	8 polysaccharide lyase	1481:1502	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	13	71	from	mechanisms	2517:2526	arg1	cycling					2546:2552	cycling	2546:2552	cycling	2546:2552	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	13	71	from	mechanisms	2517:2526	arg1	conversion					2531:2540	conversion	2531:2540	conversion	2531:2540	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	8	72	theme	shared	1619:1624	arg1	properties					1639:1648	shared and specific properties	1619:1648	shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles	1619:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	13	73	theme	ecosystems	2599:2608	arg1	cycling					2546:2552	cycling	2546:2552	cycling	2546:2552	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	13	73	theme	ecosystems	2599:2608	arg1	conversion					2531:2540	conversion	2531:2540	conversion	2531:2540	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	0	74	with	radiata	30:36	arg1	analysis					67:74	comparative genomics analysis	46:74	comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi	46:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	8	75	theme	saprobic	1691:1698	arg1	lifestyles					1711:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	1	76	theme	efficient	230:238	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	76	theme	efficient	230:238	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	76	theme	efficient	230:238	arg1	modifier					287:294	modifier	287:294	modifier of softwood and hardwood lignin	287:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	76	theme	efficient	230:238	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	13	77	theme	in the	2585:2590	arg1	ecosystems					2599:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	10	78	theme	radiata	1883:1889	arg1	genome					1891:1896	P. radiata genome	1880:1896	P. radiata genome	1880:1896	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	8	79	theme	specific	1630:1637	arg1	properties					1639:1648	shared and specific properties	1619:1648	shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles	1619:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	11	80	theme	P.	2067:2068	arg1	genome					2078:2083	P. radiata genome	2067:2083	P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins	2067:2175	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	7	81	theme	carbohydrate	1458:1469	arg1	esterase					1471:1478	27 carbohydrate esterase	1455:1478	27 carbohydrate esterase	1455:1478	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	3	82	from	wood	632:635	arg1	Finland					646:652	Finland	646:652	Finland	646:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	10	83	theme	metabolites	1966:1976	arg1	production					1942:1951	the production	1938:1951	the production of secondary metabolites	1938:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	13	84	theme	organic	2570:2576	arg1	ecosystems					2599:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	recalcitrant organic carbon in the forest ecosystems	2557:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	11	85	theme	predicted	2025:2033	arg1	proteins					2039:2046	predicted ABC proteins	2025:2046	predicted ABC proteins	2025:2046	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	4	86	theme	specific	813:820	arg1	enzymes					842:848	specific carbohydrate-active enzymes	813:848	specific carbohydrate-active enzymes (CAZymes)	813:858	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	86	theme	specific	813:820	arg1	CAZymes					851:857	CAZymes	851:857	CAZymes	851:857	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	6	87	theme	sequenced	1169:1177	arg1	genome					1187:1192	The PACBio sequenced nuclear genome	1158:1192	The PACBio sequenced nuclear genome of P. radiata	1158:1206	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	6	88	theme	sequencing	1245:1254	arg1	coverage					1256:1263	72X sequencing coverage	1241:1263	72X sequencing coverage	1241:1263	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	3	89	theme	genome	552:557	arg1	sequence					559:566	the genome sequence	548:566	the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland	548:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	11	90	theme	secreted	2159:2166	arg1	proteins					2168:2175	small secreted proteins	2153:2175	small secreted proteins	2153:2175	In addition, 49 genes encoding predicted ABC proteins were identified in P. radiata genome together with 336 genes encoding peptidases, and 430 genes encoding small secreted proteins.
31138126	12	91	theme	radiata	2216:2222	arg1	assembly					2201:2208	The genome assembly	2190:2208	The genome assembly of P. radiata	2190:2222	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	6	92	dep	sequenced	1169:1177	arg1	PACBio					1162:1167	PACBio	1162:1167	PACBio	1162:1167	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	5	93	theme	secondary	1075:1083	arg1	metabolism					1085:1094	secondary metabolism	1075:1094	secondary metabolism	1075:1094	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	3	94	theme	radiata	574:580	arg1	isolate					582:588	P. radiata isolate 79	571:591	P. radiata isolate 79 originally isolated from decayed alder wood in South Finland	571:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	12	95	dep	CONCLUSIONS	2178:2188	arg1	serve					2410:2414	serve	2410:2414	may thus serve as reference for further studies	2401:2447	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	12	95	dep	CONCLUSIONS	2178:2188	arg1	contains					2224:2231	contains	2224:2231	contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition	2224:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	2	96	theme	biomass	524:530	arg1	utilization					503:513	sustainable utilization	491:513	sustainable utilization of plant biomass	491:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	0	97	from	analysis	67:74	arg1	machinery					108:116	lignocellulose decomposition machinery	79:116	lignocellulose decomposition machinery of phlebioid fungi	79:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	7	98	theme	functional	1388:1397	arg1	annotation					1399:1408	functional annotation	1388:1408	functional annotation	1388:1408	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	13	99	theme	decay	2511:2515	arg1	mechanisms					2517:2526	enlightening fungal decay mechanisms	2491:2526	enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems	2491:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	1	100	theme	waste	373:377	arg1	substrates					394:403	various waste lignocellulose substrates	365:403	various waste lignocellulose substrates	365:403	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	8	101	with	Comparisons	1556:1566	arg1	genomes					1577:1583	the genomes	1573:1583	the genomes of other phlebioid fungi	1573:1608	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	9	102	theme	AA9	1757:1759	arg1	genes					1777:1781	especially GH10 and AA9 enzyme-encoding genes	1737:1781	especially GH10 and AA9 enzyme-encoding genes according to genomic localization	1737:1815	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	12	103	theme	rot	2360:2362	arg1	lifestyle					2364:2372	phlebioid white rot lifestyle	2344:2372	phlebioid white rot lifestyle in wood decomposition	2344:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	10	104	theme	P.	1880:1881	arg1	genome					1891:1896	P. radiata genome	1880:1896	P. radiata genome	1880:1896	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	4	105	from	clustering	784:793	arg1	species					892:898	seven closely related fungal species	863:898	seven closely related fungal species	863:898	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	3	106	attach	isolated	604:611	arg1	wood					632:635	decayed alder wood	618:635	decayed alder wood in South Finland	618:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	3	106	attach	isolated	604:611	arg2	isolate					582:588	P. radiata isolate 79	571:591	P. radiata isolate 79 originally isolated from decayed alder wood in South Finland	571:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	4	107	theme	fungal	885:890	arg1	species					892:898	seven closely related fungal species	863:898	seven closely related fungal species	863:898	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	108	theme	Polyporales	738:748	arg1	clade					760:764	the Polyporales phlebioid clade	734:764	the Polyporales phlebioid clade	734:764	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	109	theme	decay	698:702	arg1	mechanisms					704:713	wood decay mechanisms	693:713	wood decay mechanisms	693:713	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	110	from	content	772:778	arg1	species					892:898	seven closely related fungal species	863:898	seven closely related fungal species	863:898	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	1	111	theme	white	153:157	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	111	theme	white	153:157	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	111	theme	white	153:157	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	111	theme	white	153:157	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	12	112	theme	identifiable	2327:2338	arg1	composition					2315:2325	a composition	2313:2325	a composition identifiable for phlebioid white rot lifestyle in wood decomposition	2313:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	6	113	theme	P.	1197:1198	arg1	radiata					1200:1206	P. radiata	1197:1206	P. radiata	1197:1206	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	6	114	theme	protein-coding	1353:1366	arg1	genes					1368:1372	approximately 14 082 predicted protein-coding genes	1322:1372	approximately 14 082 predicted protein-coding genes	1322:1372	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	0	115	theme	lignocellulose	79:92	arg1	machinery					108:116	lignocellulose decomposition machinery	79:116	lignocellulose decomposition machinery of phlebioid fungi	79:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	1	116	theme	fungus	163:168	arg1	decomposer					240:249	an efficient decomposer	227:249	an efficient decomposer of plant cell wall polysaccharides	227:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	116	theme	fungus	163:168	arg1	radiata					178:184	The white rot fungus Phlebia radiata	149:184	The white rot fungus Phlebia radiata	149:184	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	116	theme	fungus	163:168	arg1	species					194:200	a type species	187:200	a type species of the genus Phlebia	187:221	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	1	116	theme	fungus	163:168	arg1	able					336:339	able	336:339	able	336:339	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	12	117	theme	genes	2304:2308	arg1	array					2238:2242	wide array	2233:2242	wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes	2233:2308	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	2	118	theme	biotechnological	451:466	arg1	applications					468:479	biotechnological applications	451:479	biotechnological applications aiming at sustainable utilization of plant biomass	451:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	12	119	theme	phlebioid	2344:2352	arg1	lifestyle					2364:2372	phlebioid white rot lifestyle	2344:2372	phlebioid white rot lifestyle in wood decomposition	2344:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	5	120	theme	radiata	1141:1147	arg1	assembly					1126:1133	the genome assembly	1115:1133	the genome assembly of P. radiata	1115:1147	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	1	121	dep	softwood	299:306	arg1	lignin					321:326	lignin	321:326	lignin	321:326	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	12	122	theme	polymer	2260:2266	arg1	CAZyme					2278:2283	carbohydrate polymer attacking CAZyme	2247:2283	carbohydrate polymer attacking CAZyme	2247:2283	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	0	123	theme	phlebioid	121:129	arg1	fungi					131:135	phlebioid fungi	121:135	phlebioid fungi	121:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	13	124	theme	enlightening	2491:2502	arg1	mechanisms					2517:2526	enlightening fungal decay mechanisms	2491:2526	enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems	2491:2608	Comparative genomics also contributed to enlightening fungal decay mechanisms in conversion and cycling of recalcitrant organic carbon in the forest ecosystems.
31138126	2	125	theme	sustainable	491:501	arg1	utilization					503:513	sustainable utilization	491:513	sustainable utilization of plant biomass	491:530	Thus, P. radiata is a promising organism for biotechnological applications aiming at sustainable utilization of plant biomass.
31138126	4	126	theme	phlebioid	750:758	arg1	clade					760:764	the Polyporales phlebioid clade	734:764	the Polyporales phlebioid clade	734:764	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	6	127	theme	predicted	1343:1351	arg1	genes					1368:1372	approximately 14 082 predicted protein-coding genes	1322:1372	approximately 14 082 predicted protein-coding genes	1322:1372	RESULTS The PACBio sequenced nuclear genome of P. radiata was assembled to 93 contigs with 72X sequencing coverage and annotated, revealing a dense genome of 40.4 Mbp with approximately 14 082 predicted protein-coding genes.
31138126	5	128	theme	genome	1119:1124	arg1	assembly					1126:1133	the genome assembly	1115:1133	the genome assembly of P. radiata	1115:1147	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	12	129	theme	wide	2233:2236	arg1	array					2238:2242	wide array	2233:2242	wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes	2233:2308	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	0	130	theme	Phlebia	22:28	arg1	radiata					30:36	Phlebia radiata 79	22:39	Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi	22:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	1	131	theme	wall	265:268	arg1	polysaccharides					270:284	plant cell wall polysaccharides	254:284	plant cell wall polysaccharides	254:284	BACKGROUND The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste lignocellulose substrates.
31138126	5	132	theme	other	931:935	arg1	genes					937:941	other genes	931:941	other genes encoding proteins reflecting the fungal lifestyle including peptidases	931:1012	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	9	133	theme	phlebioid	1858:1866	arg1	species					1868:1874	the phlebioid species	1854:1874	the phlebioid species	1854:1874	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	12	134	contain	contains	2224:2231	arg1	assembly					2201:2208	The genome assembly	2190:2208	The genome assembly of P. radiata	2190:2222	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	12	134	contain	contains	2224:2231	arg2	assembly					2201:2208	The genome assembly	2190:2208	The genome assembly of P. radiata	2190:2222	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	12	134	contain	contains	2224:2231	arg1	composition					2315:2325	a composition	2313:2325	a composition identifiable for phlebioid white rot lifestyle in wood decomposition	2313:2394	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	12	134	contain	contains	2224:2231	arg2	array					2238:2242	wide array	2233:2242	wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes	2233:2308	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	8	135	with	species	1660:1666	arg1	lifestyles					1711:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	3	136	theme	decayed	618:624	arg1	wood					632:635	decayed alder wood	618:635	decayed alder wood in South Finland	618:652	Here we report the genome sequence of P. radiata isolate 79 originally isolated from decayed alder wood in South Finland.
31138126	0	137	theme	comparative	46:56	arg1	analysis					67:74	comparative genomics analysis	46:74	comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi	46:135	Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.
31138126	7	138	theme	redox	1527:1531	arg1	genes					1549:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	over 70 auxiliary redox enzyme-encoding genes	1509:1553	According to functional annotation, the genome harbors 209 glycoside hydrolase, 27 carbohydrate esterase, 8 polysaccharide lyase, and over 70 auxiliary redox enzyme-encoding genes.
31138126	5	139	theme	small	1029:1033	arg1	proteins					1044:1051	small secreted proteins	1029:1051	small secreted proteins	1029:1051	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	9	140	theme	GH10	1748:1751	arg1	genes					1777:1781	especially GH10 and AA9 enzyme-encoding genes	1737:1781	especially GH10 and AA9 enzyme-encoding genes according to genomic localization	1737:1815	Clustering of especially GH10 and AA9 enzyme-encoding genes according to genomic localization was discovered to be conserved among the phlebioid species.
31138126	8	141	theme	phlebioid	1594:1602	arg1	fungi					1604:1608	other phlebioid fungi	1588:1608	other phlebioid fungi	1588:1608	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	10	142	theme	genes	1920:1924	arg1	repertoire					1906:1915	a rich repertoire	1899:1915	a rich repertoire of genes involved in the production of secondary metabolites	1899:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	10	142	theme	genes	1920:1924	arg1	genes					1920:1924	genes	1920:1924	genes involved in the production of secondary metabolites	1920:1976	In P. radiata genome, a rich repertoire of genes involved in the production of secondary metabolites was recognized.
31138126	4	143	theme	genes	798:802	arg1	content					772:778	gene content	767:778	gene content	767:778	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	4	143	theme	genes	798:802	arg1	clustering					784:793	clustering	784:793	clustering	784:793	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
31138126	5	144	theme	lifestyle	983:991	arg1	peptidases					1003:1012	the fungal lifestyle including peptidases	972:1012	proteins reflecting the fungal lifestyle including peptidases	952:1012	In addition, other genes encoding proteins reflecting the fungal lifestyle including peptidases, transporters, small secreted proteins and genes involved in secondary metabolism were identified in the genome assembly of P. radiata.
31138126	12	145	theme	CAZyme	2278:2283	arg1	array					2238:2242	wide array	2233:2242	wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes	2233:2308	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	8	146	theme	similar	1683:1689	arg1	lifestyles					1711:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	8	147	theme	wood-decay	1700:1709	arg1	lifestyles					1711:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	seemingly similar saprobic wood-decay lifestyles	1673:1720	Comparisons with the genomes of other phlebioid fungi revealed shared and specific properties among the species with seemingly similar saprobic wood-decay lifestyles.
31138126	12	148	theme	genome	2194:2199	arg1	assembly					2201:2208	The genome assembly	2190:2208	The genome assembly of P. radiata	2190:2222	CONCLUSIONS The genome assembly of P. radiata contains wide array of carbohydrate polymer attacking CAZyme and oxidoreductase genes in a composition identifiable for phlebioid white rot lifestyle in wood decomposition, and may thus serve as reference for further studies.
31138126	4	149	from	evolution	680:688	arg1	fungus					723:728	this fungus	718:728	this fungus	718:728	To better understand the evolution of wood decay mechanisms in this fungus and the Polyporales phlebioid clade, gene content and clustering of genes encoding specific carbohydrate-active enzymes (CAZymes) in seven closely related fungal species was investigated.
30064770	1	0	theme	incubation	154:163	arg1	temperatures					165:176	different incubation temperatures	144:176	different incubation temperatures	144:176	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	3	1	theme	V-amylose	498:506	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	4	2	from	index	598:602	arg1	composites					621:630	the composites	617:630	the composites incubated at 65 °C and 70 °C	617:659	The retention index of lutein in the composites incubated at 65 °C and 70 °C could achieve 76% after 21 days of storage.
30064770	7	3	theme	potential	1090:1098	arg1	use					1100:1102	their potential use	1084:1102	their potential use in the sensitive food supplement	1084:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	3	4	theme	increased	550:558	arg1	temperature					571:581	the increased incubation temperature	546:581	the increased incubation temperature	546:581	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	0	5	theme	in	83:84	arg1	digestion					92:100	in vitro digestion	83:100	in vitro digestion	83:100	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	6	6	theme	intestinal	872:881	arg1	digestion					883:891	the first 20 min intestinal digestion	855:891	the first 20 min intestinal digestion	855:891	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	3	7	theme	of	495:496	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	4	8	theme	retention	588:596	arg1	index					598:602	The retention index	584:602	The retention index of lutein in the composites incubated at 65 °C and 70 °C	584:659	The retention index of lutein in the composites incubated at 65 °C and 70 °C could achieve 76% after 21 days of storage.
30064770	3	9	theme	single	482:487	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	6	10	with	consistent	894:903	arg1	data					928:931	starch hydrolysis data	910:931	starch hydrolysis data	910:931	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	6	11	theme	first	859:863	arg1	digestion					883:891	the first 20 min intestinal digestion	855:891	the first 20 min intestinal digestion	855:891	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	0	12	dep	in	83:84	arg1	vitro					86:90	vitro	86:90	vitro	86:90	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	7	13	theme	vital	1063:1067	arg1	importance					1069:1078	vital importance	1063:1078	vital importance	1063:1078	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	3	14	theme	incubation	560:569	arg1	temperature					571:581	the increased incubation temperature	546:581	the increased incubation temperature	546:581	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	6	15	theme	20 min	865:870	arg1	digestion					883:891	the first 20 min intestinal digestion	855:891	the first 20 min intestinal digestion	855:891	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	3	16	dep	decreased	424:432	arg1	helix					442:446	double helix	435:446	double helix of amylopectin	435:461	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	2	17	theme	lutein	389:394	arg1	0.78-4.86 mg/g					405:418	0.78-4.86 mg/g	405:418	0.78-4.86 mg/g	405:418	Increasing incubation temperature (55-70 °C) induced the gelatinization of cornstarch, resulting in enhancement of swelling power (1.67-6.26 g/g) and lutein content (0.78-4.86 mg/g).
30064770	2	17	theme	lutein	389:394	arg1	content					396:402	lutein content	389:402	lutein content (0.78-4.86 mg/g)	389:419	Increasing incubation temperature (55-70 °C) induced the gelatinization of cornstarch, resulting in enhancement of swelling power (1.67-6.26 g/g) and lutein content (0.78-4.86 mg/g).
30064770	1	18	theme	complexation	196:207	arg1	granules					122:129	Swelled cornstarch granules	103:129	Swelled cornstarch granules incubated at different incubation temperatures	103:176	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	1	18	theme	complexation	196:207	arg1	matrix					209:214	a complexation matrix	194:214	a complexation matrix to encapsulate lutein	194:236	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	0	19	theme	lutein	17:22	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of lutein into swelled cornstarch granules	0:55	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	5	20	from	%	717:717	arg1	composites					736:745	the composites	732:745	the composites	732:745	Less than 30% of lutein in the composites was released after incubating for 120 min under stomach conditions.
30064770	0	21	dep	Structure	58:66	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of lutein into swelled cornstarch granules	0:55	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	0	22	theme	cornstarch	37:46	arg1	granules					48:55	swelled cornstarch granules	29:55	swelled cornstarch granules	29:55	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	1	23	theme	cornstarch	111:120	arg1	granules					122:129	Swelled cornstarch granules	103:129	Swelled cornstarch granules incubated at different incubation temperatures	103:176	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	1	23	theme	cornstarch	111:120	arg1	matrix					209:214	a complexation matrix	194:214	a complexation matrix to encapsulate lutein	194:236	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	2	24	theme	incubation	250:259	arg1	55-70 °C					274:281	55-70 °C	274:281	55-70 °C	274:281	Increasing incubation temperature (55-70 °C) induced the gelatinization of cornstarch, resulting in enhancement of swelling power (1.67-6.26 g/g) and lutein content (0.78-4.86 mg/g).
30064770	2	24	theme	incubation	250:259	arg1	temperature					261:271	incubation temperature	250:271	incubation temperature (55-70 °C)	250:282	Increasing incubation temperature (55-70 °C) induced the gelatinization of cornstarch, resulting in enhancement of swelling power (1.67-6.26 g/g) and lutein content (0.78-4.86 mg/g).
30064770	3	25	theme	double	435:440	arg1	helix					442:446	double helix	435:446	double helix of amylopectin	435:461	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	3	26	theme	amylopectin	451:461	arg1	helix					442:446	double helix	435:446	double helix of amylopectin	435:461	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	0	27	theme	swelled	29:35	arg1	granules					48:55	swelled cornstarch granules	29:55	swelled cornstarch granules	29:55	Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion.
30064770	7	28	theme	sensitive	1111:1119	arg1	supplement					1126:1135	the sensitive food supplement	1107:1135	the sensitive food supplement	1107:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	7	29	from	use	1100:1102	arg1	supplement					1126:1135	the sensitive food supplement	1107:1135	the sensitive food supplement	1107:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	7	30	theme	bioactive	1027:1035	arg1	ingredients					1037:1047	bioactive ingredients	1027:1047	bioactive ingredients	1027:1047	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	7	31	theme	delivery	1006:1013	arg1	carriers					1015:1022	delivery carriers	1006:1022	delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement	1006:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	1	32	theme	Swelled	103:109	arg1	granules					122:129	Swelled cornstarch granules	103:129	Swelled cornstarch granules incubated at different incubation temperatures	103:176	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	1	32	theme	Swelled	103:109	arg1	matrix					209:214	a complexation matrix	194:214	a complexation matrix to encapsulate lutein	194:236	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	7	33	theme	food	1121:1124	arg1	supplement					1126:1135	the sensitive food supplement	1107:1135	the sensitive food supplement	1107:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	6	34	theme	hydrolysis	917:926	arg1	data					928:931	starch hydrolysis data	910:931	starch hydrolysis data	910:931	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	7	35	theme	ingredients	1037:1047	arg1	carriers					1015:1022	delivery carriers	1006:1022	delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement	1006:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	6	36	theme	lutein	820:825	arg1	release					827:833	Fast lutein release	815:833	Fast lutein release	815:833	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	7	37	theme	cornstarch	983:992	arg1	granules					994:1001	swelled cornstarch granules	975:1001	swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement	975:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	3	38	theme	helix	489:493	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	2	39	theme	cornstarch	314:323	arg1	gelatinization					296:309	the gelatinization	292:309	the gelatinization of cornstarch	292:323	Increasing incubation temperature (55-70 °C) induced the gelatinization of cornstarch, resulting in enhancement of swelling power (1.67-6.26 g/g) and lutein content (0.78-4.86 mg/g).
30064770	6	40	theme	starch	910:915	arg1	data					928:931	starch hydrolysis data	910:931	starch hydrolysis data	910:931	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	4	41	theme	storage	696:702	arg1	21 days					685:691	21 days	685:691	21 days of storage	685:702	The retention index of lutein in the composites incubated at 65 °C and 70 °C could achieve 76% after 21 days of storage.
30064770	6	42	theme	Fast	815:818	arg1	release					827:833	Fast lutein release	815:833	Fast lutein release	815:833	Fast lutein release was observed during the first 20 min intestinal digestion, consistent with starch hydrolysis data.
30064770	3	43	theme	increased	471:479	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
30064770	4	44	theme	lutein	607:612	arg1	index					598:602	The retention index	584:602	The retention index of lutein in the composites incubated at 65 °C and 70 °C	584:659	The retention index of lutein in the composites incubated at 65 °C and 70 °C could achieve 76% after 21 days of storage.
30064770	5	45	theme	stomach	795:801	arg1	conditions					803:812	stomach conditions	795:812	stomach conditions	795:812	Less than 30% of lutein in the composites was released after incubating for 120 min under stomach conditions.
30064770	1	46	theme	different	144:152	arg1	temperatures					165:176	different incubation temperatures	144:176	different incubation temperatures	144:176	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	5	47	theme	lutein	722:727	arg1	%					717:717	Less than 30%	705:717	Less than 30% of lutein in the composites	705:745	Less than 30% of lutein in the composites was released after incubating for 120 min under stomach conditions.
30064770	5	47	theme	lutein	722:727	arg1	lutein					722:727	lutein	722:727	lutein	722:727	Less than 30% of lutein in the composites was released after incubating for 120 min under stomach conditions.
30064770	7	48	theme	swelled	975:981	arg1	granules					994:1001	swelled cornstarch granules	975:1001	swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement	975:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	1	49	dep	matrix	209:214	arg1	encapsulate					219:229	encapsulate	219:229	to encapsulate lutein	216:236	Swelled cornstarch granules incubated at different incubation temperatures was prepared as a complexation matrix to encapsulate lutein.
30064770	7	50	theme	promising	953:961	arg1	pathway					963:969	a promising pathway	951:969	a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement	951:1135	This study opens a promising pathway for swelled cornstarch granules as delivery carriers of bioactive ingredients, which are of vital importance for their potential use in the sensitive food supplement.
30064770	3	51	theme	enthalpy	509:516	arg1	change					518:523	an increased (single helix of V-amylose) enthalpy change	468:523	an increased (single helix of V-amylose) enthalpy change	468:523	A decreased (double helix of amylopectin) and an increased (single helix of V-amylose) enthalpy change were associated with the increased incubation temperature.
31816374	2	0	with	scaffold	387:394	arg1	properties					409:418	optimum properties	401:418	optimum properties	401:418	In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes.
31816374	4	1	theme	composite	844:852	arg1	scaffolds					854:862	the composite scaffolds	840:862	the composite scaffolds	840:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	3	2	used	used	624:627	arg2	materials					605:613	main materials	600:613	main materials that are used to boost the mechanical and rheological properties of the scaffolds	600:695	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	8	3	theme	mechanical	1439:1448	arg1	properties					1450:1459	the mechanical properties	1435:1459	the mechanical properties	1435:1459	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	9	4	theme	engineering	1742:1752	arg1	applications					1754:1765	nasal cartilage tissue engineering applications	1719:1765	nasal cartilage tissue engineering applications	1719:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	9	5	theme	mechanical	1641:1650	arg1	attributes					1665:1674	simultaneously enhanced mechanical and cellular attributes	1617:1674	simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications	1617:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	4	6	theme	scaffolds	854:862	arg1	properties					826:835	the mechanical and biomedical properties	796:835	the mechanical and biomedical properties of the composite scaffolds	796:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	1	7	theme	cellular	271:278	arg1	adhesion					280:287	cellular adhesion	271:287	not only cellular adhesion but also mechanical properties of the treated tissues	262:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	3	8	theme	scaffolds	687:695	arg1	properties					669:678	the mechanical and rheological properties	638:678	the mechanical and rheological properties of the scaffolds	638:695	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	7	9	theme	final	1382:1386	arg1	scaffold					1388:1395	the final scaffold	1378:1395	the final scaffold	1378:1395	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	6	10	theme	attachments	1222:1232	arg1	improvement					1177:1187	the improvement	1173:1187	the improvement of the structure and better cell attachments on the scaffolds	1173:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	4	11	theme	ratios	756:761	arg1	effect					727:732	the effect	723:732	the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds	723:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	7	12	attach	presence	1285:1292	arg1	structure					1325:1333	chitosan/agarose structure	1308:1333	chitosan/agarose structure	1308:1333	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	7	12	attach	presence	1285:1292	arg2	ECM					1301:1303	the ECM	1297:1303	the ECM	1297:1303	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	4	13	theme	weight	749:754	arg1	ratios					756:761	the various weight ratios	737:761	the various weight ratios of chitosan, agarose, and ECM	737:791	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	3	14	theme	scaffolds	556:564	arg1	properties					538:547	the cellular properties	525:547	the cellular properties of the scaffolds	525:564	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	7	15	theme	ECM	1301:1303	arg1	presence					1285:1292	the presence	1281:1292	the presence of the ECM in chitosan/agarose structure	1281:1333	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	5	16	theme	rheological	1083:1093	arg1	characteristics					1095:1109	rheological characteristics	1083:1109	rheological characteristics	1083:1109	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	4	17	theme	biomedical	815:824	arg1	properties					826:835	the mechanical and biomedical properties	796:835	the mechanical and biomedical properties of the composite scaffolds	796:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	4	18	theme	chitosan	766:773	arg1	ratios					756:761	the various weight ratios	737:761	the various weight ratios of chitosan, agarose, and ECM	737:791	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	7	19	theme	chitosan/agarose	1308:1323	arg1	structure					1325:1333	chitosan/agarose structure	1308:1333	chitosan/agarose structure	1308:1333	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	3	20	theme	Cartilage	459:467	arg1	ECM					491:493	ECM	491:493	ECM	491:493	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	3	20	theme	Cartilage	459:467	arg1	matrix					483:488	Cartilage extracellular matrix	459:488	Cartilage extracellular matrix (ECM)	459:494	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	8	21	theme	resulting	1549:1557	arg1	interactions					1559:1570	their resulting interactions	1543:1570	their resulting interactions	1543:1570	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	9	22	theme	cellular	1656:1663	arg1	attributes					1665:1674	simultaneously enhanced mechanical and cellular attributes	1617:1674	simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications	1617:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	5	23	theme	mechanical	961:970	arg1	strength					972:979	good mechanical strength	956:979	good mechanical strength	956:979	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	2	24	theme	optimum	401:407	arg1	properties					409:418	optimum properties	401:418	optimum properties	401:418	In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes.
31816374	5	25	theme	partial	1001:1007	arg1	crystallinity					1009:1021	partial crystallinity	1001:1021	partial crystallinity	1001:1021	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	9	26	theme	nasal	1719:1723	arg1	applications					1754:1765	nasal cartilage tissue engineering applications	1719:1765	nasal cartilage tissue engineering applications	1719:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	6	27	from	improvement	1177:1187	arg1	scaffolds					1241:1249	the scaffolds	1237:1249	the scaffolds	1237:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	1	28	theme	porous	195:200	arg1	scaffolds					220:228	porous three-dimensional scaffolds	195:228	porous three-dimensional scaffolds	195:228	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	6	29	theme	structure	1196:1204	arg1	improvement					1177:1187	the improvement	1173:1187	the improvement of the structure and better cell attachments on the scaffolds	1173:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	8	30	theme	scaffolds	1487:1495	arg1	microstructure					1465:1478	microstructure	1465:1478	microstructure	1465:1478	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	8	30	theme	scaffolds	1487:1495	arg1	properties					1450:1459	the mechanical properties	1435:1459	the mechanical properties	1435:1459	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	1	31	theme	three-dimensional	202:218	arg1	scaffolds					220:228	porous three-dimensional scaffolds	195:228	porous three-dimensional scaffolds	195:228	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	0	32	theme	scaffolds	32:40	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan/agarose scaffolds	0:40	Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications.
31816374	1	33	theme	mechanical	298:307	arg1	properties					309:318	mechanical properties	298:318	not only cellular adhesion but also mechanical properties of the treated tissues	262:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	9	34	theme	tissue	1735:1740	arg1	applications					1754:1765	nasal cartilage tissue engineering applications	1719:1765	nasal cartilage tissue engineering applications	1719:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	4	35	from	effect	727:732	arg1	properties					826:835	the mechanical and biomedical properties	796:835	the mechanical and biomedical properties of the composite scaffolds	796:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	4	36	theme	various	741:747	arg1	ratios					756:761	the various weight ratios	737:761	the various weight ratios of chitosan, agarose, and ECM	737:791	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	2	37	theme	cartilage	437:445	arg1	attributes					447:456	nasal cartilage attributes	431:456	nasal cartilage attributes	431:456	In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes.
31816374	0	38	theme	chitosan/agarose	15:30	arg1	scaffolds					32:40	chitosan/agarose scaffolds	15:40	chitosan/agarose scaffolds	15:40	Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications.
31816374	5	39	theme	good	956:959	arg1	strength					972:979	good mechanical strength	956:979	good mechanical strength	956:979	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	40	theme	controlled	1045:1054	arg1	rate					1073:1076	controlled biodegradability rate	1045:1076	controlled biodegradability rate	1045:1076	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	morphology					989:998	porous morphology	982:998	porous morphology	982:998	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	ratio					1038:1042	high swelling ratio	1024:1042	high swelling ratio	1024:1042	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	characteristics					1095:1109	rheological characteristics	1083:1109	rheological characteristics	1083:1109	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	crystallinity					1009:1021	partial crystallinity	1001:1021	partial crystallinity	1001:1021	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	range					925:929	a range	923:929	a range	923:929	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	strength					972:979	good mechanical strength	956:979	good mechanical strength	956:979	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	5	41	theme	advantages	934:943	arg1	rate					1073:1076	controlled biodegradability rate	1045:1076	controlled biodegradability rate	1045:1076	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	2	42	theme	nasal	431:435	arg1	attributes					447:456	nasal cartilage attributes	431:456	nasal cartilage attributes	431:456	In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes.
31816374	0	43	theme	extracellular	53:65	arg1	matrix					67:72	extracellular matrix	53:72	extracellular matrix for tissue engineering applications	53:108	Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications.
31816374	5	44	theme	resulting	894:902	arg1	composites					904:913	The resulting composites	890:913	The resulting composites	890:913	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	9	45	theme	cartilage	1725:1733	arg1	applications					1754:1765	nasal cartilage tissue engineering applications	1719:1765	nasal cartilage tissue engineering applications	1719:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	3	46	theme	mechanical	642:651	arg1	properties					669:678	the mechanical and rheological properties	638:678	the mechanical and rheological properties of the scaffolds	638:695	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	3	47	used	used	500:503	arg2	ECM					491:493	ECM	491:493	ECM	491:493	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	3	47	used	used	500:503	arg2	matrix					483:488	Cartilage extracellular matrix	459:488	Cartilage extracellular matrix (ECM)	459:494	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	8	48	from	able	1419:1422	arg1	addition					1401:1408	addition	1401:1408	addition	1401:1408	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	6	49	from	scaffolds	1241:1249	arg1	improvement					1177:1187	the improvement	1173:1187	the improvement of the structure and better cell attachments on the scaffolds	1173:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	3	50	theme	main	600:603	arg1	materials					605:613	main materials	600:613	main materials that are used to boost the mechanical and rheological properties of the scaffolds	600:695	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	7	51	theme	scaffold	1388:1395	arg1	characteristics					1359:1373	the biomedical characteristics	1344:1373	the biomedical characteristics of the final scaffold	1344:1395	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	6	52	dep	structure	1196:1204	arg1	the					1192:1194	the	1192:1194	the	1192:1194	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	1	53	theme	treated	327:333	arg1	tissues					335:341	the treated tissues	323:341	the treated tissues	323:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	4	54	theme	Taguchi	874:880	arg1	method					882:887	the Taguchi method	870:887	the Taguchi method	870:887	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	4	55	theme	mechanical	800:809	arg1	properties					826:835	the mechanical and biomedical properties	796:835	the mechanical and biomedical properties of the composite scaffolds	796:862	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	1	56	theme	effective	127:135	arg1	approaches					137:146	the most effective approaches	118:146	the most effective approaches for treatment of cartilage	118:173	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	1	57	theme	tissues	335:341	arg1	properties					309:318	mechanical properties	298:318	not only cellular adhesion but also mechanical properties of the treated tissues	262:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	1	57	theme	tissues	335:341	arg1	adhesion					280:287	cellular adhesion	271:287	not only cellular adhesion but also mechanical properties of the treated tissues	262:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	5	58	theme	high	1024:1027	arg1	ratio					1038:1042	high swelling ratio	1024:1042	high swelling ratio	1024:1042	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	6	59	theme	cell	1217:1220	arg1	attachments					1222:1232	better cell attachments	1210:1232	better cell attachments	1210:1232	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	1	60	theme	approaches	137:146	arg1	approaches					137:146	the most effective approaches	118:146	the most effective approaches for treatment of cartilage	118:173	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	1	60	theme	approaches	137:146	arg1	One					111:113	One	111:113	One	111:113	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	6	61	from	attachments	1222:1232	arg1	scaffolds					1241:1249	the scaffolds	1237:1249	the scaffolds	1237:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	7	62	from	presence	1285:1292	arg1	structure					1325:1333	chitosan/agarose structure	1308:1333	chitosan/agarose structure	1308:1333	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	9	63	theme	enhanced	1632:1639	arg1	attributes					1665:1674	simultaneously enhanced mechanical and cellular attributes	1617:1674	simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications	1617:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	5	64	theme	swelling	1029:1036	arg1	ratio					1038:1042	high swelling ratio	1024:1042	high swelling ratio	1024:1042	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	4	65	theme	agarose	776:782	arg1	ratios					756:761	the various weight ratios	737:761	the various weight ratios of chitosan, agarose, and ECM	737:791	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	4	66	theme	ECM	789:791	arg1	ratios					756:761	the various weight ratios	737:761	the various weight ratios of chitosan, agarose, and ECM	737:791	Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method.
31816374	6	67	theme	cytotoxicity	1143:1154	arg1	tests					1156:1160	the cytotoxicity tests	1139:1160	the cytotoxicity tests	1139:1160	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	9	68	with	scaffold	1603:1610	arg1	attributes					1665:1674	simultaneously enhanced mechanical and cellular attributes	1617:1674	simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications	1617:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	6	69	theme	better	1210:1215	arg1	attachments					1222:1232	better cell attachments	1210:1232	better cell attachments	1210:1232	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31816374	8	70	from	addition	1401:1408	arg1	able					1419:1422	able	1419:1422	able	1419:1422	In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions.
31816374	5	71	theme	biodegradability	1056:1071	arg1	rate					1073:1076	controlled biodegradability rate	1045:1076	controlled biodegradability rate	1045:1076	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	3	72	theme	cellular	529:536	arg1	properties					538:547	the cellular properties	525:547	the cellular properties of the scaffolds	525:564	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	7	73	theme	biomedical	1348:1357	arg1	characteristics					1359:1373	the biomedical characteristics	1344:1373	the biomedical characteristics of the final scaffold	1344:1395	Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold.
31816374	0	74	theme	engineering	85:95	arg1	applications					97:108	tissue engineering applications	78:108	tissue engineering applications	78:108	Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications.
31816374	3	75	theme	rheological	657:667	arg1	properties					669:678	the mechanical and rheological properties	638:678	the mechanical and rheological properties of the scaffolds	638:695	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	5	76	theme	porous	982:987	arg1	morphology					989:998	porous morphology	982:998	porous morphology	982:998	The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics.
31816374	1	77	theme	scaffolds	220:228	arg1	use					188:190	the use	184:190	the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues	184:341	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	2	78	theme	composite	377:385	arg1	scaffold					387:394	a composite scaffold	375:394	a composite scaffold with optimum properties	375:418	In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes.
31816374	3	79	theme	extracellular	469:481	arg1	ECM					491:493	ECM	491:493	ECM	491:493	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	3	79	theme	extracellular	469:481	arg1	matrix					483:488	Cartilage extracellular matrix	459:488	Cartilage extracellular matrix (ECM)	459:494	Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds.
31816374	0	80	theme	tissue	78:83	arg1	applications					97:108	tissue engineering applications	78:108	tissue engineering applications	78:108	Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications.
31816374	9	81	theme	novel	1597:1601	arg1	scaffold					1603:1610	a novel scaffold	1595:1610	a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications	1595:1765	These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications.
31816374	1	82	theme	cartilage	165:173	arg1	treatment					152:160	treatment	152:160	treatment of cartilage	152:173	One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues.
31816374	6	83	from	structure	1196:1204	arg1	scaffolds					1241:1249	the scaffolds	1237:1249	the scaffolds	1237:1249	Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds.
31218857	3	0	theme	METHODS	348:354	arg1	groups					362:367	METHODS Three groups	348:367	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	348:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	3	0	theme	METHODS	348:354	arg1	hydrogels					391:399	CMCS/OSA composite hydrogels	372:399	CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	372:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	6	1	theme	amine-to-aldhyde	966:981	arg1	ratio					983:987	a amine-to-aldhyde ratio	964:987	a amine-to-aldhyde ratio of 1∶1	964:994	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	5	2	from	scaffolds	849:857	arg1	engineering					879:889	cartilage tissue engineering	862:889	cartilage tissue engineering	862:889	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	6	3	with	hydrogel	950:957	arg1	ratio					983:987	a amine-to-aldhyde ratio	964:987	a amine-to-aldhyde ratio of 1∶1	964:994	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	4	theme	adhesive	1045:1052	arg1	force					1054:1058	strong adhesive force	1038:1058	strong adhesive force	1038:1058	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	0	5	theme	tissue	93:98	arg1	engineering					100:110	cartilage tissue engineering	83:110	cartilage tissue engineering	83:110	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	4	6	theme	groups	546:551	arg1	biocompatibility					516:531	cell biocompatibility	511:531	cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels	511:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	4	6	theme	groups	546:551	arg1	microstructure					470:483	The microstructure	466:483	The microstructure	466:483	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	4	6	theme	groups	546:551	arg1	properties					495:504	physical properties	486:504	physical properties	486:504	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	7	7	contain	has	1217:1219	arg2	applications					1231:1242	potential applications	1221:1242	potential applications	1221:1242	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	7	7	contain	has	1217:1219	arg1	hydrogel					1153:1160	The CMCS/OSA compound hydrogel	1131:1160	The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups	1131:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	5	8	theme	electron	637:644	arg1	microscopy					646:655	scanning electron microscopy	628:655	scanning electron microscopy	628:655	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	1	9	theme	carboxymethyl	172:184	arg1	hydrogels					231:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	6	10	theme	strong	1038:1043	arg1	force					1054:1058	strong adhesive force	1038:1058	strong adhesive force	1038:1058	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	3	11	theme	hydrogels	391:399	arg1	groups					362:367	METHODS Three groups	348:367	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	348:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	3	11	theme	hydrogels	391:399	arg1	hydrogels					391:399	CMCS/OSA composite hydrogels	372:399	CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	372:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	5	12	theme	swelling	699:706	arg1	test					713:716	swelling rate test	699:716	swelling rate test	699:716	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	12	theme	swelling	699:706	arg1	test					670:673	rheological test	658:673	rheological test	658:673	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	13	theme	composite	765:773	arg1	hydrogel					775:782	the CMCS/OSA composite hydrogel	752:782	the CMCS/OSA composite hydrogel	752:782	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	14	theme	rate	708:711	arg1	test					713:716	swelling rate test	699:716	swelling rate test	699:716	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	14	theme	rate	708:711	arg1	test					670:673	rheological test	658:673	rheological test	658:673	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	6	15	theme	1∶1	992:994	arg1	ratio					983:987	a amine-to-aldhyde ratio	964:987	a amine-to-aldhyde ratio of 1∶1	964:994	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	2	16	theme	hydrogels	304:312	arg1	applicability					283:295	the applicability	279:295	the applicability of the hydrogels in cartilage tissue engi-neering	279:345	This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.
31218857	3	17	theme	CMCS/OSA	372:379	arg1	hydrogels					391:399	CMCS/OSA composite hydrogels	372:399	CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	372:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	6	18	theme	CMCS/OSA	941:948	arg1	hydrogel					950:957	the CMCS/OSA hydrogel	937:957	the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1	937:994	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	7	19	theme	compound	1144:1151	arg1	hydrogel					1153:1160	The CMCS/OSA compound hydrogel	1131:1160	The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups	1131:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	5	20	theme	rheological	658:668	arg1	test					670:673	rheological test	658:673	rheological test	658:673	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	20	theme	rheological	658:668	arg1	test					713:716	swelling rate test	699:716	swelling rate test	699:716	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	20	theme	rheological	658:668	arg1	test					693:696	adhesion tension test	676:696	adhesion tension test	676:696	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	1	21	theme	chitosan/sodium	186:200	arg1	hydrogels					231:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	3	22	theme	composite	381:389	arg1	hydrogels					391:399	CMCS/OSA composite hydrogels	372:399	CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	372:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	7	23	theme	amino	1191:1195	arg1	groups					1210:1215	amino and aldehyde groups	1191:1215	groups	1210:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	7	24	theme	cartilage	1247:1255	arg1	engineering					1264:1274	cartilage tissue engineering	1247:1274	cartilage tissue engineering	1247:1274	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	7	25	theme	CMCS/OSA	1135:1142	arg1	hydrogel					1153:1160	The CMCS/OSA compound hydrogel	1131:1160	The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups	1131:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	1	26	theme	alginate	202:209	arg1	hydrogels					231:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	7	27	dep	CONCLUSIONS	1119:1129	arg1	has					1217:1219	has	1217:1219	has potential applications in cartilage tissue engineering	1217:1274	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	4	28	theme	cell	511:514	arg1	biocompatibility					516:531	cell biocompatibility	511:531	cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels	511:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	5	29	theme	adhesion	676:683	arg1	test					670:673	rheological test	658:673	rheological test	658:673	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	29	theme	adhesion	676:683	arg1	test					693:696	adhesion tension test	676:696	adhesion tension test	676:696	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	3	30	theme	1∶1	439:441	arg1	ratios					424:429	amino-to-aldehyde ratios	406:429	amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	406:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	3	31	theme	2∶1	434:436	arg1	ratios					424:429	amino-to-aldehyde ratios	406:429	amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	406:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	6	32	theme	gelling	1024:1030	arg1	time					1032:1035	suitable gelling time	1015:1035	suitable gelling time	1015:1035	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	5	33	theme	tension	685:691	arg1	test					670:673	rheological test	658:673	rheological test	658:673	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	33	theme	tension	685:691	arg1	test					693:696	adhesion tension test	676:696	adhesion tension test	676:696	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	1	34	theme	CMCS/OSA	212:219	arg1	hydrogels					231:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	3	35	theme	amino-to-aldehyde	406:422	arg1	ratios					424:429	amino-to-aldehyde ratios	406:429	amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	406:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	6	36	theme	suitable	1015:1022	arg1	time					1032:1035	suitable gelling time	1015:1035	suitable gelling time	1015:1035	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	5	37	theme	scanning	628:635	arg1	microscopy					646:655	scanning electron microscopy	628:655	scanning electron microscopy	628:655	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	2	38	theme	tissue	327:332	arg1	engi-neering					334:345	cartilage tissue engi-neering	317:345	cartilage tissue engi-neering	317:345	This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.
31218857	5	39	theme	tissue	872:877	arg1	engineering					879:889	cartilage tissue engineering	862:889	cartilage tissue engineering	862:889	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	5	40	theme	cartilage	862:870	arg1	engineering					879:889	cartilage tissue engineering	862:889	cartilage tissue engineering	862:889	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	6	41	dep	RESULTS	892:898	arg1	showed					925:930	showed	925:930	showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility	925:1116	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	2	42	theme	cartilage	317:325	arg1	engi-neering					334:345	cartilage tissue engi-neering	317:345	cartilage tissue engi-neering	317:345	This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.
31218857	2	43	from	applicability	283:295	arg1	engi-neering					334:345	cartilage tissue engi-neering	317:345	cartilage tissue engi-neering	317:345	This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.
31218857	1	44	theme	compound	222:229	arg1	hydrogels					231:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	172:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	0	45	theme	chitosan/sodium	45:59	arg1	hydrogel					70:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	7	46	theme	potential	1221:1229	arg1	applications					1231:1242	potential applications	1221:1242	potential applications	1221:1242	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	6	47	theme	cellular	1092:1099	arg1	biocompatibility					1101:1116	good cellular biocompatibility	1087:1116	good cellular biocompatibility	1087:1116	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	1	48	theme	hydrogels	231:239	arg1	preparation					157:167	the preparation	153:167	the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	153:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	0	49	theme	carboxymethyl	31:43	arg1	hydrogel					70:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	4	50	theme	CMCS/OSA	556:563	arg1	hydrogels					576:584	CMCS/OSA com-posite hydrogels	556:584	CMCS/OSA com-posite hydrogels	556:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	3	51	theme	1∶2	447:449	arg1	ratios					424:429	amino-to-aldehyde ratios	406:429	amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	406:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	6	52	theme	experimental	904:915	arg1	results					917:923	The experimental results	900:923	The experimental results	900:923	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	7	53	theme	aldehyde	1201:1208	arg1	groups					1210:1215	amino and aldehyde groups	1191:1215	groups	1210:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	0	54	theme	hydrogel	70:77	arg1	evaluation					17:26	evaluation	17:26	evaluation	17:26	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	0	54	theme	hydrogel	70:77	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation	0:11	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	5	55	theme	CMCS/OSA	756:763	arg1	hydrogel					775:782	the CMCS/OSA composite hydrogel	752:782	the CMCS/OSA composite hydrogel	752:782	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	4	56	theme	physical	486:493	arg1	properties					495:504	physical properties	486:504	physical properties	486:504	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	3	57	with	hydrogels	391:399	arg1	ratios					424:429	amino-to-aldehyde ratios	406:429	amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2	406:449	METHODS Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared.
31218857	0	58	theme	alginate	61:68	arg1	hydrogel					70:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	carboxymethyl chitosan/sodium alginate hydrogel	31:77	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	4	59	theme	hydrogels	576:584	arg1	hydrogels					576:584	CMCS/OSA com-posite hydrogels	556:584	CMCS/OSA com-posite hydrogels	556:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	4	59	theme	hydrogels	576:584	arg1	groups					546:551	the three groups	536:551	the three groups of CMCS/OSA com-posite hydrogels	536:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	7	60	theme	1∶1	1178:1180	arg1	ratio					1182:1186	a 1∶1 ratio	1176:1186	a 1∶1 ratio of amino and aldehyde groups	1176:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	4	61	theme	com-posite	565:574	arg1	hydrogels					576:584	CMCS/OSA com-posite hydrogels	556:584	CMCS/OSA com-posite hydrogels	556:584	The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated.
31218857	6	62	theme	good	1000:1003	arg1	porosity					1005:1012	good porosity	1000:1012	good porosity	1000:1012	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	63	theme	swelling	1068:1075	arg1	rate					1077:1080	stable swelling rate	1061:1080	stable swelling rate	1061:1080	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	5	64	theme	cell	723:726	arg1	experiments					728:738	cell experiments	723:738	cell experiments	723:738	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	6	65	theme	stable	1061:1066	arg1	rate					1077:1080	stable swelling rate	1061:1080	stable swelling rate	1061:1080	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	0	66	theme	cartilage	83:91	arg1	engineering					100:110	cartilage tissue engineering	83:110	cartilage tissue engineering	83:110	[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].
31218857	6	67	contain	had	996:998	arg2	biocompatibility					1101:1116	good cellular biocompatibility	1087:1116	good cellular biocompatibility	1087:1116	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	67	contain	had	996:998	arg2	time					1032:1035	suitable gelling time	1015:1035	suitable gelling time	1015:1035	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	67	contain	had	996:998	arg1	hydrogel					950:957	the CMCS/OSA hydrogel	937:957	the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1	937:994	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	67	contain	had	996:998	arg2	porosity					1005:1012	good porosity	1000:1012	good porosity	1000:1012	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	67	contain	had	996:998	arg2	rate					1077:1080	stable swelling rate	1061:1080	stable swelling rate	1061:1080	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	67	contain	had	996:998	arg2	force					1054:1058	strong adhesive force	1038:1058	strong adhesive force	1038:1058	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	6	68	theme	good	1087:1090	arg1	biocompatibility					1101:1116	good cellular biocompatibility	1087:1116	good cellular biocompatibility	1087:1116	RESULTS The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
31218857	1	69	dep	OBJECTIVE	114:122	arg1	aimed					135:139	aimed	135:139	aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels	135:239	OBJECTIVE This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels.
31218857	5	70	theme	cross-linking	793:805	arg1	degree					807:812	the cross-linking degree	789:812	the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering	789:889	Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
31218857	7	71	theme	groups	1210:1215	arg1	ratio					1182:1186	a 1∶1 ratio	1176:1186	a 1∶1 ratio of amino and aldehyde groups	1176:1215	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
31218857	7	72	theme	tissue	1257:1262	arg1	engineering					1264:1274	cartilage tissue engineering	1247:1274	cartilage tissue engineering	1247:1274	CONCLUSIONS The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
30719943	5	0	theme	2:3	779:781	arg1	w.w-1					783:787	2:3 w.w-1	779:787	2:3 w.w-1	779:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	1	theme	Encapsulation	828:840	arg1	EY					849:850	EY	849:850	EY	849:850	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	1	theme	Encapsulation	828:840	arg1	yield					842:846	Encapsulation yield	828:846	Encapsulation yield (EY) of 83%	828:858	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	2	2	theme	Central	369:375	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	2	2	theme	Central	369:375	arg1	Design					398:403	Central Composite Rotational Design	369:403	Central Composite Rotational Design (CCRD)	369:410	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	5	3	theme	w.w-1	783:787	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	3	theme	w.w-1	783:787	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	4	4	theme	negative	527:534	arg1	effect					536:541	a negative effect	525:541	a negative effect	525:541	Temperature and feed flow rate had a negative effect.
30719943	8	5	theme	La-05	1209:1213	arg1	properties					1169:1178	well-encapsulating properties	1150:1178	well-encapsulating properties of Lactobacillus acidophilus La-05	1150:1213	Soy extract/maltodextrin presented well-encapsulating properties of Lactobacillus acidophilus La-05.
30719943	4	6	contain	had	521:523	arg2	effect					536:541	a negative effect	525:541	a negative effect	525:541	Temperature and feed flow rate had a negative effect.
30719943	4	6	contain	had	521:523	arg1	Temperature					490:500	Temperature	490:500	Temperature	490:500	Temperature and feed flow rate had a negative effect.
30719943	4	6	contain	had	521:523	arg1	rate					516:519	feed flow rate	506:519	feed flow rate	506:519	Temperature and feed flow rate had a negative effect.
30719943	5	7	dep	8.82 μm	582:588	arg1	to					579:580	to	579:580	to	579:580	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	8	theme	87 °C	738:742	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	8	theme	87 °C	738:742	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	8	theme	87 °C	738:742	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	2	9	theme	maltodextrin/soy	293:308	arg1	ratio					318:322	maltodextrin/soy extract ratio	293:322	maltodextrin/soy extract ratio	293:322	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	0	10	theme	model	91:95	arg1	approach					97:104	a model approach	89:104	a model approach	89:104	Soy extract and maltodextrin as microencapsulating agents for Lactobacillus acidophilus: a model approach.
30719943	6	11	theme	FTIR	882:885	arg1	analysis					887:894	FTIR analysis	882:894	FTIR analysis	882:894	Thermogravimetry and FTIR analysis suggested that microcapsules could protect L. acidophilus cells against dehydration and heating.
30719943	5	12	theme	maltodextrin/soy	745:760	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	12	theme	maltodextrin/soy	745:760	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	7	13	theme	log	1102:1104	arg1	cycles					1106:1111	0.12 and 1.72 log cycles	1088:1111	0.12 and 1.72 log cycles	1088:1111	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	0	14	dep	approach	97:104	arg1	maltodextrin					16:27	maltodextrin	16:27	maltodextrin	16:27	Soy extract and maltodextrin as microencapsulating agents for Lactobacillus acidophilus: a model approach.
30719943	0	14	dep	approach	97:104	arg1	extract					4:10	Soy extract	0:10	Soy extract	0:10	Soy extract and maltodextrin as microencapsulating agents for Lactobacillus acidophilus: a model approach.
30719943	5	15	theme	extract	762:768	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	15	theme	extract	762:768	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	4	16	theme	flow	511:514	arg1	rate					516:519	feed flow rate	506:519	feed flow rate	506:519	Temperature and feed flow rate had a negative effect.
30719943	2	17	theme	inlet	274:278	arg1	temperature					280:290	Air inlet temperature	270:290	Air inlet temperature	270:290	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	7	18	contain	had	1037:1039	arg1	probiotic					1027:1035	probiotic	1027:1035	probiotic	1027:1035	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	7	18	contain	had	1037:1039	arg2	viability					1051:1059	high cell viability	1041:1059	high cell viability	1041:1059	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	4	19	theme	feed	506:509	arg1	rate					516:519	feed flow rate	506:519	feed flow rate	506:519	Temperature and feed flow rate had a negative effect.
30719943	2	20	theme	feed	328:331	arg1	rate					338:341	feed flow rate	328:341	feed flow rate	328:341	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	7	21	dep	had	1037:1039	arg1	reductions					1062:1071	reductions	1062:1071	reductions ranged between 0.12 and 1.72 log cycles	1062:1111	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	1	22	theme	La-05	195:199	arg1	microencapsulation					147:164	the microencapsulation	143:164	the microencapsulation of Lactobacillus acidophilus La-05 by spray drying	143:215	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	0	23	theme	Soy	0:2	arg1	extract					4:10	Soy extract	0:10	Soy extract	0:10	Soy extract and maltodextrin as microencapsulating agents for Lactobacillus acidophilus: a model approach.
30719943	5	24	theme	water	591:595	arg1	activity					597:604	water activity	591:604	water activity	591:604	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	25	theme	%	858:858	arg1	EY					849:850	EY	849:850	EY	849:850	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	25	theme	%	858:858	arg1	yield					842:846	Encapsulation yield	828:846	Encapsulation yield (EY) of 83%	828:858	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	26	theme	0.54 L.h-1	808:817	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	26	theme	0.54 L.h-1	808:817	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	1	27	theme	spray	204:208	arg1	drying					210:215	spray drying	204:215	spray drying	204:215	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	5	28	dep	7.01 g.100g-1	650:662	arg1	to					647:648	to	647:648	to	647:648	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	29	theme	air	719:721	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	29	theme	air	719:721	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	29	theme	air	719:721	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	2	30	theme	extract	310:316	arg1	ratio					318:322	maltodextrin/soy extract ratio	293:322	maltodextrin/soy extract ratio	293:322	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	0	31	theme	microencapsulating	32:49	arg1	agents					51:56	microencapsulating agents	32:56	microencapsulating agents for Lactobacillus acidophilus	32:86	Soy extract and maltodextrin as microencapsulating agents for Lactobacillus acidophilus: a model approach.
30719943	5	32	theme	feed	790:793	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	32	theme	feed	790:793	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	33	from	Particles	664:672	arg1	moisture					628:635	moisture	628:635	moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1)	628:818	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	3	34	theme	soy	477:479	arg1	extract					481:487	soy extract	477:487	soy extract	477:487	Probiotic viability increased with increasing the proportion of soy extract.
30719943	5	35	theme	flow	795:798	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	35	theme	flow	795:798	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	6	36	theme	acidophilus	942:952	arg1	cells					954:958	L. acidophilus cells	939:958	L. acidophilus cells	939:958	Thermogravimetry and FTIR analysis suggested that microcapsules could protect L. acidophilus cells against dehydration and heating.
30719943	7	37	theme	high	1041:1044	arg1	viability					1051:1059	high cell viability	1041:1059	high cell viability	1041:1059	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	1	38	theme	present	111:117	arg1	study					119:123	The present study	107:123	The present study	107:123	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	3	39	theme	Probiotic	413:421	arg1	viability					423:431	Probiotic viability	413:431	Probiotic viability	413:431	Probiotic viability increased with increasing the proportion of soy extract.
30719943	5	40	theme	optimised	697:705	arg1	conditions					707:716	the optimised conditions	693:716	the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1)	693:818	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	2	41	theme	Air	270:272	arg1	temperature					280:290	Air inlet temperature	270:290	Air inlet temperature	270:290	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	8	42	theme	Soy	1115:1117	arg1	extract/maltodextrin					1119:1138	Soy extract/maltodextrin	1115:1138	Soy extract/maltodextrin	1115:1138	Soy extract/maltodextrin presented well-encapsulating properties of Lactobacillus acidophilus La-05.
30719943	5	43	dep	0.52	619:622	arg1	to					616:617	to	616:617	to	616:617	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	1	44	theme	soy	224:226	arg1	encapsulants					256:267	encapsulants	256:267	encapsulants	256:267	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	1	44	theme	soy	224:226	arg1	maltodextrin					240:251	maltodextrin	240:251	maltodextrin	240:251	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	1	44	theme	soy	224:226	arg1	extract					228:234	soy extract	224:234	soy extract	224:234	The present study aimed to optimise the microencapsulation of Lactobacillus acidophilus La-05 by spray drying, using soy extract and maltodextrin as encapsulants.
30719943	7	45	theme	cell	1046:1049	arg1	viability					1051:1059	high cell viability	1041:1059	high cell viability	1041:1059	During storage, microencapsulated probiotic had high cell viability (reductions ranged between 0.12 and 1.72 log cycles).
30719943	2	46	theme	flow	333:336	arg1	rate					338:341	feed flow rate	328:341	feed flow rate	328:341	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	8	47	theme	well-encapsulating	1150:1167	arg1	properties					1169:1178	well-encapsulating properties	1150:1178	well-encapsulating properties of Lactobacillus acidophilus La-05	1150:1213	Soy extract/maltodextrin presented well-encapsulating properties of Lactobacillus acidophilus La-05.
30719943	3	48	theme	extract	481:487	arg1	proportion					463:472	the proportion	459:472	the proportion of soy extract	459:487	Probiotic viability increased with increasing the proportion of soy extract.
30719943	2	49	theme	Rotational	387:396	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	2	49	theme	Rotational	387:396	arg1	Design					398:403	Central Composite Rotational Design	369:403	Central Composite Rotational Design (CCRD)	369:410	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	5	50	theme	Particle	544:551	arg1	diameter					553:560	Particle diameter	544:560	Particle diameter	544:560	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	2	51	theme	Composite	377:385	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	2	51	theme	Composite	377:385	arg1	Design					398:403	Central Composite Rotational Design	369:403	Central Composite Rotational Design (CCRD)	369:410	Air inlet temperature, maltodextrin/soy extract ratio and feed flow rate were investigated through Central Composite Rotational Design (CCRD).
30719943	5	52	dep	conditions	707:716	arg1	rate					800:803	feed flow rate	790:803	feed flow rate of 0.54 L.h-1	790:817	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	52	dep	conditions	707:716	arg1	temperature					723:733	air temperature	719:733	air temperature of 87 °C	719:742	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30719943	5	52	dep	conditions	707:716	arg1	ratio					770:774	maltodextrin/soy extract ratio	745:774	maltodextrin/soy extract ratio of 2:3 w.w-1	745:787	Particle diameter ranged from 4.97 to 8.82 μm, water activity from 0.25 to 0.52 and moisture from 2.30 to 7.01 g.100g-1 Particles produced following the optimised conditions (air temperature of 87 °C, maltodextrin/soy extract ratio of 2:3 w.w-1, feed flow rate of 0.54 L.h-1) reached Encapsulation yield (EY) of 83%.
30445869	6	0	theme	first	1250:1254	arg1	time					1256:1259	the first time	1246:1259	the first time	1246:1259	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	5	1	theme	2,2-diphenyl-1-picrylhydrazyl	975:1003	arg1	radical					1005:1011	2,2-diphenyl-1-picrylhydrazyl radical	975:1011	2,2-diphenyl-1-picrylhydrazyl radical	975:1011	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	6	2	from	stress	1135:1140	arg1	patients					1145:1152	patients	1145:1152	patients with diabetes	1145:1166	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	0	3	theme	root	70:73	arg1	antioxidant					15:25	antioxidant	15:25	antioxidant	15:25	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	0	3	theme	root	70:73	arg1	Antiglycation					0:12	Antiglycation	0:12	Antiglycation	0:12	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	0	3	theme	root	70:73	arg1	activities					42:51	cytotoxic activities	32:51	cytotoxic activities	32:51	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	5	4	theme	radical	1005:1011	arg1	assays					1039:1044	2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays	975:1044	2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays	975:1044	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	4	5	theme	brine	754:758	arg1	shrimp					760:765	brine shrimp	754:765	brine shrimp	754:765	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	5	6	dep	IC50	922:925	arg1	0.292					951:955	absorbance 0.292	940:955	absorbance 0.292	940:955	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	0	7	theme	oil	89:91	arg1	composition					93:103	essential oil composition	79:103	essential oil composition	79:103	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	4	8	theme	significant	679:689	arg1	activity					705:712	significant antiglycation activity	679:712	significant antiglycation activity (IC50, 1.12 mg/mL)	679:731	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	4	9	theme	LC50	768:771	arg1	cytotoxicity					738:749	cytotoxicity	738:749	cytotoxicity to brine shrimp (LC50, 25.01 µg/mL)	738:785	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	4	9	theme	LC50	768:771	arg1	25.01 µg/mL					774:784	LC50, 25.01 µg/mL	768:784	25.01 µg/mL	774:784	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	2	10	theme	root	250:253	arg1	extracts					255:262	The root extracts	246:262	The root extracts	246:262	The root extracts were evaluated for composition, antiglycation, antioxidant, and cytotoxicity.
30445869	1	11	theme	folkloric	218:226	arg1	therapy					237:243	folkloric diabetic therapy	218:243	folkloric diabetic therapy	218:243	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	0	12	theme	essential	79:87	arg1	composition					93:103	essential oil composition	79:103	essential oil composition	79:103	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	3	13	theme	chromatography-mass	604:622	arg1	GC-MS					638:642	GC-MS	638:642	GC-MS	638:642	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	3	13	theme	chromatography-mass	604:622	arg1	spectrometry					624:635	gas chromatography-mass spectrometry	600:635	gas chromatography-mass spectrometry (GC-MS)	600:643	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	6	14	theme	antiglycation	1083:1095	arg1	effect					1097:1102	antiglycation effect	1083:1102	antiglycation effect	1083:1102	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	5	15	dep	oil	917:919	arg1	100 µg/mL					958:966	100 µg/mL	958:966	100 µg/mL	958:966	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	15	dep	oil	917:919	arg1	50.0 µg/mL					928:937	50.0 µg/mL	928:937	50.0 µg/mL	928:937	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	15	dep	oil	917:919	arg1	IC50					922:925	IC50	922:925	IC50	922:925	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	16	dep	IC50	801:804	arg1	0.531					829:833	absorbance 0.531	818:833	absorbance 0.531	818:833	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	1	17	theme	diabetic	228:235	arg1	therapy					237:243	folkloric diabetic therapy	218:243	folkloric diabetic therapy	218:243	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	18	theme	essential	140:148	arg1	plant					162:166	an essential oil bearing plant	137:166	an essential oil bearing plant	137:166	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	18	theme	essential	140:148	arg1	chamae					113:118	Uvaria chamae	106:118	Uvaria chamae	106:118	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	0	19	theme	composition	93:103	arg1	antioxidant					15:25	antioxidant	15:25	antioxidant	15:25	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	0	19	theme	composition	93:103	arg1	Antiglycation					0:12	Antiglycation	0:12	Antiglycation	0:12	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	0	19	theme	composition	93:103	arg1	activities					42:51	cytotoxic activities	32:51	cytotoxic activities	32:51	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	5	20	dep	extract	792:798	arg1	100 µg/mL					836:844	100 µg/mL	836:844	100 µg/mL	836:844	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	20	dep	extract	792:798	arg1	8.0 µg/mL					807:815	8.0 µg/mL	807:815	8.0 µg/mL	807:815	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	20	dep	extract	792:798	arg1	IC50					801:804	IC50	801:804	IC50	801:804	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	1	21	theme	oil	150:152	arg1	plant					162:166	an essential oil bearing plant	137:166	an essential oil bearing plant	137:166	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	21	theme	oil	150:152	arg1	chamae					113:118	Uvaria chamae	106:118	Uvaria chamae	106:118	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	3	22	theme	gas	600:602	arg1	GC-MS					638:642	GC-MS	638:642	GC-MS	638:642	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	3	22	theme	gas	600:602	arg1	spectrometry					624:635	gas chromatography-mass spectrometry	600:635	gas chromatography-mass spectrometry (GC-MS)	600:643	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	6	23	theme	oxidative	1125:1133	arg1	stress					1135:1140	oxidative stress	1125:1140	oxidative stress in patients with diabetes	1125:1166	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	1	24	theme	bearing	154:160	arg1	plant					162:166	an essential oil bearing plant	137:166	an essential oil bearing plant	137:166	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	24	theme	bearing	154:160	arg1	chamae					113:118	Uvaria chamae	106:118	Uvaria chamae	106:118	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	3	25	theme	cardiac	354:360	arg1	glycosides					362:371	cardiac glycosides	354:371	cardiac glycosides	354:371	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	5	26	theme	absorbance	818:827	arg1	0.531					829:833	absorbance 0.531	818:833	absorbance 0.531	818:833	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	0	27	theme	cytotoxic	32:40	arg1	activities					42:51	cytotoxic activities	32:51	cytotoxic activities	32:51	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	5	28	theme	ferric	1017:1022	arg1	power					1033:1037	ferric reducing power	1017:1037	ferric reducing power	1017:1037	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	6	29	theme	oil	1195:1197	arg1	composition					1199:1209	oil composition	1195:1209	oil composition	1195:1209	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	3	30	theme	alcohol	414:420	arg1	extract					422:428	the alcohol extract	410:428	the alcohol extract	410:428	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	5	31	theme	reducing	1024:1031	arg1	power					1033:1037	ferric reducing power	1017:1037	ferric reducing power	1017:1037	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	32	theme	absorbance	940:949	arg1	0.292					951:955	absorbance 0.292	940:955	absorbance 0.292	940:955	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	1	33	theme	Uvaria	106:111	arg1	Annonaceae					121:130	Annonaceae	121:130	Annonaceae	121:130	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	33	theme	Uvaria	106:111	arg1	chamae					113:118	Uvaria chamae	106:118	Uvaria chamae	106:118	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	33	theme	Uvaria	106:111	arg1	plant					162:166	an essential oil bearing plant	137:166	an essential oil bearing plant	137:166	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	5	34	theme	power	1033:1037	arg1	assays					1039:1044	2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays	975:1044	2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays	975:1044	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	5	35	theme	essential	907:915	arg1	oil					917:919	the essential oil	903:919	the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL)	903:967	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	3	36	from	extract	422:428	arg1	high					402:405	high	402:405	high	402:405	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	6	37	theme	antiglycation	1173:1185	arg1	effect					1187:1192	its antiglycation effect	1169:1192	its antiglycation effect	1169:1192	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	3	38	theme	gas	572:574	arg1	GC					592:593	GC	592:593	GC	592:593	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	3	38	theme	gas	572:574	arg1	chromatography					576:589	gas chromatography	572:589	gas chromatography (GC)	572:594	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	4	39	theme	antiglycation	691:703	arg1	activity					705:712	significant antiglycation activity	679:712	significant antiglycation activity (IC50, 1.12 mg/mL)	679:731	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	3	40	theme	benzyl	431:436	arg1	benzoate					438:445	benzyl benzoate	431:445	benzyl benzoate (23.3%)	431:453	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	3	40	theme	benzyl	431:436	arg1	%					452:452	23.3%	448:452	23.3%	448:452	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	6	41	with	patients	1145:1152	arg1	diabetes					1159:1166	diabetes	1159:1166	diabetes	1159:1166	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	4	42	theme	ethanol	650:656	arg1	extract					658:664	The ethanol extract	646:664	The ethanol extract	646:664	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	0	43	theme	chamae	63:68	arg1	root					70:73	Uvaria chamae root	56:73	Uvaria chamae root	56:73	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	6	44	dep	U.	1060:1061	arg1	chamae					1063:1068	U. chamae	1060:1068	U. chamae	1060:1068	U. chamae root possess antiglycation effect, and may also reduce oxidative stress in patients with diabetes; its antiglycation effect, oil composition, and cytotoxicity are reported for the first time.
30445869	3	45	theme	methyl	507:512	arg1	thymol					514:519	methyl thymol	507:519	methyl thymol (8.7%)	507:526	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	3	45	theme	methyl	507:512	arg1	%					525:525	8.7%	522:525	8.7%	522:525	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	7	46	dep	[Formula	1261:1268	arg1	see					1271:1273	see	1271:1273	see text	1271:1278	[Formula: see text].
30445869	4	47	dep	activity	705:712	arg1	1.12 mg/mL					721:730	1.12 mg/mL	721:730	1.12 mg/mL	721:730	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	4	47	dep	activity	705:712	arg1	IC50					715:718	IC50	715:718	IC50	715:718	The ethanol extract demonstrated significant antiglycation activity (IC50, 1.12 mg/mL), and cytotoxicity to brine shrimp (LC50, 25.01 µg/mL).
30445869	0	48	theme	Uvaria	56:61	arg1	root					70:73	Uvaria chamae root	56:73	Uvaria chamae root	56:73	Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition.
30445869	5	49	theme	antioxidant	869:879	arg1	effects					881:887	better antioxidant effects	862:887	better antioxidant effects	862:887	The extract (IC50, 8.0 µg/mL; absorbance 0.531, 100 µg/mL) also exhibited better antioxidant effects compared with the essential oil (IC50, 50.0 µg/mL; absorbance 0.292, 100 µg/mL) using 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power assays respectively.
30445869	3	50	from	high	402:405	arg1	extract					422:428	the alcohol extract	410:428	the alcohol extract	410:428	Flavonoids, cardiac glycosides, and tannins were relatively high in the alcohol extract; benzyl benzoate (23.3%), dimethoxy-p-cymene (14.2%), τ-cadinol (12.1%), and methyl thymol (8.7%) predominated the constituents identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
30445869	1	51	theme	effective	197:205	arg1	root					173:176	root	173:176	root	173:176	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
30445869	1	51	theme	effective	197:205	arg1	remedy					207:212	an effective remedy	194:212	an effective remedy for folkloric diabetic therapy	194:243	Uvaria chamae (Annonaceae), is an essential oil bearing plant; the root is acclaimed as an effective remedy for folkloric diabetic therapy.
29621637	3	0	theme	bioactive	523:531	arg1	materials					543:551	polysaccharide-based bioactive polymeric materials	502:551	polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons	502:698	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	1	1	theme	neuronal	244:251	arg1	regrowth					260:267	neuronal tissue regrowth	244:267	neuronal tissue regrowth	244:267	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	0	2	theme	neurons	81:87	arg1	bio-constructs					55:68	in vitro bio-constructs	46:68	in vitro bio-constructs of primary neurons and progenitor cells	46:108	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	5	3	theme	neuronal	951:958	arg1	growth					960:965	hippocampal neuronal growth	939:965	hippocampal neuronal growth	939:965	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	3	4	theme	polymeric	533:541	arg1	materials					543:551	polysaccharide-based bioactive polymeric materials	502:551	polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons	502:698	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	1	5	theme	tissue	253:258	arg1	regrowth					260:267	neuronal tissue regrowth	244:267	neuronal tissue regrowth	244:267	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	0	6	theme	cells	104:108	arg1	bio-constructs					55:68	in vitro bio-constructs	46:68	in vitro bio-constructs of primary neurons and progenitor cells	46:108	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	9	7	theme	scaffolds	1653:1661	arg1	exploitation					1616:1627	the exploitation	1612:1627	the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction	1612:1708	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	10	8	theme	innovative	1852:1861	arg1	approach					1863:1870	an innovative approach	1849:1870	an innovative approach for central nervous system repair	1849:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	8	9	theme	complex	1515:1521	arg1	bio-constructs					1523:1536	complex bio-constructs	1515:1536	complex bio-constructs promoting central nervous system regeneration	1515:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	3	10	theme	ideal	569:573	arg1	composite					575:583	the ideal composite	565:583	the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons	565:698	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	11	theme	neuronal	596:603	arg1	growth					613:618	neuronal network growth	596:618	neuronal network growth	596:618	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	0	12	theme	progenitor	93:102	arg1	cells					104:108	progenitor cells	93:108	progenitor cells	93:108	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	4	13	theme	able	812:815	arg1	cells					806:810	engineered mesenchymal stem cells	778:810	engineered mesenchymal stem cells able to release neurotrophic factors	778:847	Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors.
29621637	3	14	used	used	434:437	arg2	We					431:432	We	431:432	We	431:432	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	10	15	theme	cell	1831:1834	arg1	therapies					1836:1844	stem cell therapies	1826:1844	stem cell therapies as an innovative approach for central nervous system repair	1826:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	10	16	theme	system	1892:1897	arg1	repair					1899:1904	central nervous system repair	1876:1904	central nervous system repair	1876:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	4	17	theme	mesenchymal	789:799	arg1	cells					806:810	engineered mesenchymal stem cells	778:810	engineered mesenchymal stem cells able to release neurotrophic factors	778:847	Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors.
29621637	4	18	theme	neurotrophic	828:839	arg1	factors					841:847	neurotrophic factors	828:847	neurotrophic factors	828:847	Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors.
29621637	10	19	theme	biomaterial	1790:1800	arg1	scaffolds					1802:1810	biomaterial scaffolds	1790:1810	biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair	1790:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	9	20	theme	polysaccharide-based	1632:1651	arg1	scaffolds					1653:1661	polysaccharide-based scaffolds	1632:1661	polysaccharide-based scaffolds able to favour neuronal network reconstruction	1632:1708	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	5	21	theme	neuronal	1041:1048	arg1	lineage					1050:1056	the proper neuronal lineage	1030:1056	the proper neuronal lineage	1030:1056	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	6	22	theme	SIGNIFICANCE	1144:1155	arg1	STATEMENT					1131:1139	STATEMENT	1131:1139	STATEMENT	1131:1139	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	5	23	theme	Chitosan	912:919	arg1	Chitlac					901:907	Chitlac	901:907	Chitlac	901:907	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	5	23	theme	Chitosan	912:919	arg1	derivative					921:930	a Chitosan derivative	910:930	a Chitosan derivative	910:930	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	0	24	theme	natural	11:17	arg1	polysaccharides					19:33	natural polysaccharides	11:33	natural polysaccharides	11:33	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	6	25	theme	biocompatible	1231:1243	arg1	materials					1245:1253	biocompatible materials	1231:1253	biocompatible materials	1231:1253	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	7	26	theme	Chitlac-thick	1298:1310	arg1	substrates					1312:1321	Chitlac-thick substrates	1298:1321	Chitlac-thick substrates	1298:1321	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	10	27	theme	central	1876:1882	arg1	system					1892:1897	central nervous system	1876:1897	central nervous system repair	1876:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	3	28	theme	network	605:611	arg1	growth					613:618	neuronal network growth	596:618	neuronal network growth	596:618	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	7	29	theme	active	1405:1410	arg1	synapses					1412:1419	active synapses	1405:1419	active synapses	1405:1419	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	10	30	theme	scaffolds	1802:1810	arg1	design					1780:1785	the design	1776:1785	the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair	1776:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	1	31	from	strategies	119:128	arg1	repair					162:167	Central Nervous System (CNS) repair	133:167	Central Nervous System (CNS) repair	133:167	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	2	32	theme	synthetic	303:311	arg1	structures					313:322	such synthetic structures	298:322	such synthetic structures	298:322	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	8	33	theme	new	1454:1456	arg1	candidate					1482:1490	a promising candidate	1470:1490	a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration	1470:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	8	33	theme	new	1454:1456	arg1	material					1458:1465	this new material	1449:1465	this new material	1449:1465	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	7	34	theme	synapses	1412:1419	arg1	formation					1392:1400	formation	1392:1400	formation	1392:1400	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	7	34	theme	synapses	1412:1419	arg1	maturation					1377:1386	maturation	1377:1386	maturation	1377:1386	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	7	34	theme	synapses	1412:1419	arg1	differentiation					1360:1374	differentiation	1360:1374	differentiation	1360:1374	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	7	34	theme	synapses	1412:1419	arg1	growth					1352:1357	growth	1352:1357	growth	1352:1357	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	5	35	theme	composite	868:876	arg1	bio-constructs					878:891	composite bio-constructs	868:891	composite bio-constructs made of Chitlac, a Chitosan derivative,	868:931	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	5	36	theme	local	1083:1087	arg1	delivery					1104:1111	local and continuous delivery	1083:1111	local and continuous delivery of neurotrophins	1083:1128	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	2	37	dep	therapies	339:347	arg1	encapsulating					350:362	encapsulating	350:362	encapsulating progenitor cells	350:379	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	2	37	dep	therapies	339:347	arg1	instructing					385:395	instructing	385:395	instructing their differentiation and growth	385:428	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	5	38	theme	progenitors	1013:1023	arg1	growth					960:965	hippocampal neuronal growth	939:965	hippocampal neuronal growth	939:965	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	5	38	theme	progenitors	1013:1023	arg1	formation					976:984	synapse formation	968:984	synapse formation	968:984	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	5	38	theme	progenitors	1013:1023	arg1	differentiation					994:1008	the differentiation	990:1008	the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins	990:1128	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	10	39	theme	stem	1826:1829	arg1	therapies					1836:1844	stem cell therapies	1826:1844	stem cell therapies as an innovative approach for central nervous system repair	1826:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	4	40	theme	engineered	778:787	arg1	cells					806:810	engineered mesenchymal stem cells	778:810	engineered mesenchymal stem cells able to release neurotrophic factors	778:847	Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors.
29621637	9	41	theme	novel	1589:1593	arg1	findings					1595:1602	Our novel findings	1585:1602	Our novel findings	1585:1602	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	5	42	theme	proper	1034:1039	arg1	lineage					1050:1056	the proper neuronal lineage	1030:1056	the proper neuronal lineage	1030:1056	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	3	43	theme	motor	686:690	arg1	neurons					692:698	motor neurons	686:698	motor neurons	686:698	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	1	44	theme	Current	111:117	arg1	strategies					119:128	Current strategies	111:128	Current strategies in Central Nervous System (CNS) repair	111:167	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	3	45	theme	materials	543:551	arg1	synthesis					467:475	synthesis	467:475	synthesis	467:475	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	45	theme	materials	543:551	arg1	characterization					482:497	characterization	482:497	characterization	482:497	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	45	theme	materials	543:551	arg1	design					459:464	design	459:464	design	459:464	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	0	46	theme	in	46:47	arg1	bio-constructs					55:68	in vitro bio-constructs	46:68	in vitro bio-constructs of primary neurons and progenitor cells	46:108	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	3	47	theme	polysaccharide-based	502:521	arg1	materials					543:551	polysaccharide-based bioactive polymeric materials	502:551	polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons	502:698	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	8	48	theme	central	1548:1554	arg1	system					1564:1569	central nervous system	1548:1569	central nervous system regeneration	1548:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	1	49	theme	artificial	197:206	arg1	scaffolds					208:216	artificial scaffolds	197:216	artificial scaffolds	197:216	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	5	50	theme	synapse	968:974	arg1	formation					976:984	synapse formation	968:984	synapse formation	968:984	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	1	51	theme	scaffolds	208:216	arg1	engineering					182:192	the engineering	178:192	the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth	178:267	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	5	52	theme	neurotrophins	1116:1128	arg1	delivery					1104:1111	local and continuous delivery	1083:1111	local and continuous delivery of neurotrophins	1083:1128	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	9	53	theme	able	1663:1666	arg1	scaffolds					1653:1661	polysaccharide-based scaffolds	1632:1661	polysaccharide-based scaffolds able to favour neuronal network reconstruction	1632:1708	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	8	54	theme	system	1564:1569	arg1	regeneration					1571:1582	central nervous system regeneration	1548:1582	central nervous system regeneration	1548:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	0	55	dep	in	46:47	arg1	vitro					49:53	vitro	49:53	vitro	49:53	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	6	56	theme	polysaccharide-based	1187:1206	arg1	platforms					1218:1226	polysaccharide-based bioactive platforms	1187:1226	polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering	1187:1282	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	8	57	theme	bio-constructs	1523:1536	arg1	development					1500:1510	the development	1496:1510	the development of complex bio-constructs promoting central nervous system regeneration	1496:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	6	58	theme	nerve	1259:1263	arg1	engineering					1272:1282	nerve tissue engineering	1259:1282	nerve tissue engineering	1259:1282	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	3	59	theme	synapse	621:627	arg1	formation					629:637	synapse formation	621:637	synapse formation	621:637	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	2	60	theme	cell	334:337	arg1	therapies					339:347	stem cell therapies	329:347	stem cell therapies	329:347	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	7	61	theme	neuronal	1343:1350	arg1	growth					1352:1357	growth	1352:1357	growth	1352:1357	We show that Chitlac-thick substrates are able to promote neuronal growth, differentiation, maturation and formation of active synapses.
29621637	5	62	theme	continuous	1093:1102	arg1	delivery					1104:1111	local and continuous delivery	1083:1111	local and continuous delivery of neurotrophins	1083:1128	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	3	63	dep	design	459:464	arg1	the					455:457	the	455:457	the	455:457	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	1	64	theme	Central	133:139	arg1	CNS					157:159	CNS	157:159	CNS	157:159	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	1	64	theme	Central	133:139	arg1	System					149:154	Central Nervous System	133:154	Central Nervous System (CNS) repair	133:167	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	2	65	theme	such	298:301	arg1	structures					313:322	such synthetic structures	298:322	such synthetic structures	298:322	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	10	66	theme	nervous	1884:1890	arg1	system					1892:1897	central nervous system	1876:1897	central nervous system repair	1876:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	8	67	theme	nervous	1556:1562	arg1	system					1564:1569	central nervous system	1548:1569	central nervous system regeneration	1548:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	6	68	theme	tissue	1265:1270	arg1	engineering					1272:1282	nerve tissue engineering	1259:1282	nerve tissue engineering	1259:1282	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	4	69	theme	stem	801:804	arg1	cells					806:810	engineered mesenchymal stem cells	778:810	engineered mesenchymal stem cells able to release neurotrophic factors	778:847	Moreover, we investigated the feasibility of combining these approaches with engineered mesenchymal stem cells able to release neurotrophic factors.
29621637	1	70	theme	Nervous	141:147	arg1	CNS					157:159	CNS	157:159	CNS	157:159	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	1	70	theme	Nervous	141:147	arg1	System					149:154	Central Nervous System	133:154	Central Nervous System (CNS) repair	133:167	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	2	71	theme	stem	329:332	arg1	therapies					339:347	stem cell therapies	329:347	stem cell therapies	329:347	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29621637	0	72	theme	primary	73:79	arg1	neurons					81:87	primary neurons	73:87	primary neurons	73:87	Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
29621637	5	73	theme	hippocampal	939:949	arg1	growth					960:965	hippocampal neuronal growth	939:965	hippocampal neuronal growth	939:965	We show here that composite bio-constructs made of Chitlac, a Chitosan derivative, favor hippocampal neuronal growth, synapse formation and the differentiation of progenitors into the proper neuronal lineage, that can be improved by local and continuous delivery of neurotrophins.
29621637	3	74	theme	stem	643:646	arg1	differentiation					653:667	stem cell differentiation	643:667	stem cell differentiation	643:667	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	75	from	developments	439:450	arg1	synthesis					467:475	synthesis	467:475	synthesis	467:475	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	75	from	developments	439:450	arg1	characterization					482:497	characterization	482:497	characterization	482:497	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	3	75	from	developments	439:450	arg1	design					459:464	design	459:464	design	459:464	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	1	76	theme	System	149:154	arg1	repair					162:167	Central Nervous System (CNS) repair	133:167	Central Nervous System (CNS) repair	133:167	Current strategies in Central Nervous System (CNS) repair focus on the engineering of artificial scaffolds for guiding and promoting neuronal tissue regrowth.
29621637	8	77	theme	promising	1472:1480	arg1	candidate					1482:1490	a promising candidate	1470:1490	a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration	1470:1582	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	8	77	theme	promising	1472:1480	arg1	material					1458:1465	this new material	1449:1465	this new material	1449:1465	These observations support this new material as a promising candidate for the development of complex bio-constructs promoting central nervous system regeneration.
29621637	9	78	theme	neuronal	1678:1685	arg1	reconstruction					1695:1708	neuronal network reconstruction	1678:1708	neuronal network reconstruction	1678:1708	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	10	79	theme	ideal	1756:1760	arg1	candidate					1762:1770	an ideal candidate	1753:1770	an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair	1753:1904	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	10	79	theme	ideal	1756:1760	arg1	Chitlac-thick					1732:1744	Chitlac-thick	1732:1744	Chitlac-thick	1732:1744	Our study shows that Chitlac-thick may be an ideal candidate for the design of biomaterial scaffolds enriched with stem cell therapies as an innovative approach for central nervous system repair.
29621637	6	80	theme	bioactive	1208:1216	arg1	platforms					1218:1226	polysaccharide-based bioactive platforms	1187:1226	polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering	1187:1282	STATEMENT OF SIGNIFICANCE In our work, we characterized polysaccharide-based bioactive platforms as biocompatible materials for nerve tissue engineering.
29621637	3	81	theme	cell	648:651	arg1	differentiation					653:667	stem cell differentiation	643:667	stem cell differentiation	643:667	We used developments in the design, synthesis, and characterization of polysaccharide-based bioactive polymeric materials for testing the ideal composite supporting neuronal network growth, synapse formation and stem cell differentiation into neurons and motor neurons.
29621637	9	82	theme	network	1687:1693	arg1	reconstruction					1695:1708	neuronal network reconstruction	1678:1708	neuronal network reconstruction	1678:1708	Our novel findings sustain the exploitation of polysaccharide-based scaffolds able to favour neuronal network reconstruction.
29621637	2	83	theme	progenitor	364:373	arg1	cells					375:379	progenitor cells	364:379	progenitor cells	364:379	Ideally, one should combine such synthetic structures with stem cell therapies, encapsulating progenitor cells and instructing their differentiation and growth.
29580472	3	0	theme	inner	555:559	arg1	w1					576:577	the inner water droplets w1	551:577	the inner water droplets w1	551:577	Gelation of the inner water droplets w1 increased yield by 20% for all emulsifiers.
29580472	4	1	theme	phase	700:704	arg1	viscosity					671:679	the increasing viscosity	656:679	the increasing viscosity of the outer water phase	656:704	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	4	1	theme	phase	700:704	arg1	w2					707:708	w2	707:708	w2	707:708	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	9	2	theme	double	1407:1412	arg1	emulsions					1414:1422	double emulsions	1407:1422	double emulsions	1407:1422	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	9	2	theme	double	1407:1412	arg1	replacers					1431:1439	fat replacers	1427:1439	fat replacers in more complex food systems	1427:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	8	3	theme	viscosity	1115:1123	arg1	ratio					1125:1129	viscosity ratio	1115:1129	viscosity ratio of continuous and dispersed phase	1115:1163	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	3	theme	viscosity	1115:1123	arg1	emulsifier					1067:1076	hydrophilic emulsifier	1055:1076	hydrophilic emulsifier	1055:1076	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	3	4	theme	w1	576:577	arg1	Gelation					539:546	Gelation	539:546	Gelation of the inner water droplets w1	539:577	Gelation of the inner water droplets w1 increased yield by 20% for all emulsifiers.
29580472	8	5	theme	emulsions	1254:1262	arg1	phase					1238:1242	w1 phase	1235:1242	w1 phase of double emulsions	1235:1262	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	4	6	theme	water	694:698	arg1	phase					700:704	the outer water phase	684:704	the outer water phase	684:704	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	6	7	used	used	816:819	arg2	thickener					826:834	a thickener	824:834	a thickener	824:834	When pectin was used as a thickener, in comparison to xanthan, an additional decrease in yield was observed.
29580472	6	7	used	used	816:819	arg2	pectin					805:810	pectin	805:810	pectin	805:810	When pectin was used as a thickener, in comparison to xanthan, an additional decrease in yield was observed.
29580472	7	8	theme	droplets	985:992	arg1	w1					994:995	all inner water droplets w1	969:995	all inner water droplets w1	969:995	The yield decreased to values close to zero indicating that all inner water droplets w1 were lost during emulsification.
29580472	0	9	from	Effect	0:5	arg1	droplet					47:53	oil droplet size and yield	43:68	droplet	47:53	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	0	9	from	Effect	0:5	arg1	yield					64:68	yield	64:68	yield	64:68	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	2	10	theme	droplet	444:450	arg1	sizes					452:456	smaller oil droplet sizes	432:456	smaller oil droplet sizes	432:456	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	1	11	theme	inner	317:321	arg1	phase					329:333	the inner water phase	313:333	the inner water phase	313:333	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	9	12	theme	double	1355:1360	arg1	emulsions					1362:1370	double emulsions	1355:1370	double emulsions	1355:1370	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	1	13	theme	water	263:267	arg1	phase					269:273	the outer water phase	253:273	the outer water phase	253:273	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	13	theme	water	263:267	arg1	w2					276:277	w2	276:277	w2	276:277	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	2	14	theme	oil	440:442	arg1	sizes					452:456	smaller oil droplet sizes	432:456	smaller oil droplet sizes	432:456	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	1	15	theme	water	323:327	arg1	phase					329:333	the inner water phase	313:333	the inner water phase	313:333	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	5	16	theme	lower	786:790	arg1	yields					792:797	lower yields	786:797	lower yields	786:797	This resulted in smaller oil droplets and lower yields.
29580472	7	17	theme	inner	973:977	arg1	w1					994:995	all inner water droplets w1	969:995	all inner water droplets w1	969:995	The yield decreased to values close to zero indicating that all inner water droplets w1 were lost during emulsification.
29580472	9	18	theme	fat	1427:1429	arg1	emulsions					1414:1422	double emulsions	1407:1422	double emulsions	1407:1422	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	9	18	theme	fat	1427:1429	arg1	replacers					1431:1439	fat replacers	1427:1439	fat replacers in more complex food systems	1427:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	1	19	theme	phase	329:333	arg1	w1					336:337	w1	336:337	w1	336:337	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	19	theme	phase	329:333	arg1	yield					304:308	yield	304:308	yield	304:308	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	19	theme	phase	329:333	arg1	size					295:298	oil droplet size	283:298	oil droplet size	283:298	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	0	20	theme	w1/o/w2	74:80	arg1	emulsions					90:98	(w1/o/w2) double emulsions	73:98	(w1/o/w2) double emulsions	73:98	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	3	21	theme	water	561:565	arg1	w1					576:577	the inner water droplets w1	551:577	the inner water droplets w1	551:577	Gelation of the inner water droplets w1 increased yield by 20% for all emulsifiers.
29580472	8	22	theme	continuous	1134:1143	arg1	phase					1159:1163	continuous and dispersed phase	1134:1163	continuous and dispersed phase	1134:1163	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	1	23	theme	various	129:135	arg1	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	23	theme	various	129:135	arg1	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	23	theme	various	129:135	arg1	Na-caseinate					178:189	Na-caseinate	178:189	Na-caseinate	178:189	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	23	theme	various	129:135	arg1	Tween					196:200	Tween	196:200	Tween	196:200	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	9	24	theme	better	1286:1291	arg1	understanding					1293:1305	a better understanding	1284:1305	a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems	1284:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	3	25	theme	droplets	567:574	arg1	w1					576:577	the inner water droplets w1	551:577	the inner water droplets w1	551:577	Gelation of the inner water droplets w1 increased yield by 20% for all emulsifiers.
29580472	4	26	theme	outer	688:692	arg1	phase					700:704	the outer water phase	684:704	the outer water phase	684:704	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	9	27	theme	food	1457:1460	arg1	systems					1462:1468	more complex food systems	1444:1468	more complex food systems	1444:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	8	28	theme	phase	1159:1163	arg1	ratio					1125:1129	viscosity ratio	1115:1129	viscosity ratio of continuous and dispersed phase	1115:1163	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	28	theme	phase	1159:1163	arg1	emulsifier					1067:1076	hydrophilic emulsifier	1055:1076	hydrophilic emulsifier	1055:1076	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	1	29	theme	emulsifiers	137:147	arg1	effect					119:124	the effect	115:124	the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions	115:368	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	2	30	theme	higher	462:467	arg1	yields					469:474	higher yields	462:474	higher yields	462:474	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	5	31	theme	smaller	761:767	arg1	droplets					773:780	smaller oil droplets	761:780	smaller oil droplets	761:780	This resulted in smaller oil droplets and lower yields.
29580472	9	32	used	used	1392:1395	arg2	this					1380:1383	this	1380:1383	this	1380:1383	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	0	33	theme	water	16:20	arg1	composition					28:38	outer water phase composition	10:38	outer water phase composition	10:38	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	0	34	theme	emulsions	90:98	arg1	droplet					47:53	oil droplet size and yield	43:68	droplet	47:53	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	0	34	theme	emulsions	90:98	arg1	yield					64:68	yield	64:68	yield	64:68	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	5	35	theme	oil	769:771	arg1	droplets					773:780	smaller oil droplets	761:780	smaller oil droplets	761:780	This resulted in smaller oil droplets and lower yields.
29580472	9	36	theme	emulsions	1362:1370	arg1	properties					1341:1350	the properties	1337:1350	the properties of double emulsions	1337:1370	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	1	37	theme	whey	150:153	arg1	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	37	theme	whey	150:153	arg1	WPI					172:174	WPI	172:174	WPI	172:174	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	37	theme	whey	150:153	arg1	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	0	38	theme	outer	10:14	arg1	composition					28:38	outer water phase composition	10:38	outer water phase composition	10:38	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	1	39	theme	thickeners	210:219	arg1	effect					119:124	the effect	115:124	the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions	115:368	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	0	40	theme	double	83:88	arg1	emulsions					90:98	(w1/o/w2) double emulsions	73:98	(w1/o/w2) double emulsions	73:98	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	0	41	dep	droplet	47:53	arg1	size					55:58	size	55:58	size	55:58	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	2	42	contain	had	428:430	arg2	yields					469:474	higher yields	462:474	higher yields	462:474	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	2	42	contain	had	428:430	arg1	emulsions					395:403	Double emulsions	388:403	Double emulsions stabilized by Tween 20	388:426	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	2	42	contain	had	428:430	arg2	sizes					452:456	smaller oil droplet sizes	432:456	smaller oil droplet sizes	432:456	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	1	43	theme	protein	155:161	arg1	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	43	theme	protein	155:161	arg1	WPI					172:174	WPI	172:174	WPI	172:174	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	43	theme	protein	155:161	arg1	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	0	44	theme	composition	28:38	arg1	Effect					0:5	Effect	0:5	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.	0:99	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	9	45	from	replacers	1431:1439	arg1	systems					1462:1468	more complex food systems	1444:1468	more complex food systems	1444:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	1	46	dep	emulsifiers	137:147	arg1	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	46	dep	emulsifiers	137:147	arg1	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	46	dep	emulsifiers	137:147	arg1	WPI					172:174	WPI	172:174	WPI	172:174	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	46	dep	emulsifiers	137:147	arg1	Na-caseinate					178:189	Na-caseinate	178:189	Na-caseinate	178:189	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	46	dep	emulsifiers	137:147	arg1	Tween					196:200	Tween	196:200	Tween	196:200	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	47	theme	emulsifier	1067:1076	arg1	type					1177:1180	type	1177:1180	type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener	1047:1193	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	47	theme	emulsifier	1067:1076	arg1	type					1047:1050	type	1047:1050	type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener	1047:1193	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	0	48	theme	phase	22:26	arg1	composition					28:38	outer water phase composition	10:38	outer water phase composition	10:38	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	9	49	theme	complex	1449:1455	arg1	systems					1462:1468	more complex food systems	1444:1468	more complex food systems	1444:1468	This work provides a better understanding of how composition influences the properties of double emulsions and how this can be used to design double emulsions as fat replacers in more complex food systems.
29580472	2	50	theme	Double	388:393	arg1	emulsions					395:403	Double emulsions	388:403	Double emulsions stabilized by Tween 20	388:426	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	1	51	theme	oil	283:285	arg1	droplet					287:293	oil droplet	283:293	oil droplet size	283:298	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	4	52	theme	thickeners	644:653	arg1	addition					632:639	the addition	628:639	the addition of thickeners	628:653	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	7	53	theme	water	979:983	arg1	w1					994:995	all inner water droplets w1	969:995	all inner water droplets w1	969:995	The yield decreased to values close to zero indicating that all inner water droplets w1 were lost during emulsification.
29580472	0	54	theme	oil	43:45	arg1	droplet					47:53	oil droplet size and yield	43:68	droplet	47:53	Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions.
29580472	2	55	theme	smaller	432:438	arg1	sizes					452:456	smaller oil droplet sizes	432:456	smaller oil droplet sizes	432:456	Double emulsions stabilized by Tween 20 had smaller oil droplet sizes and higher yields in comparison to emulsions stabilized by WPI and Na-caseinate.
29580472	1	56	theme	droplet	287:293	arg1	w1					336:337	w1	336:337	w1	336:337	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	56	theme	droplet	287:293	arg1	size					295:298	oil droplet size	283:298	oil droplet size	283:298	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	4	57	theme	increasing	660:669	arg1	viscosity					671:679	the increasing viscosity	656:679	the increasing viscosity of the outer water phase	656:704	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	4	57	theme	increasing	660:669	arg1	w2					707:708	w2	707:708	w2	707:708	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	1	58	theme	w1/o/w2	344:350	arg1	emulsions					360:368	(w1/o/w2) double emulsions	343:368	(w1/o/w2) double emulsions	343:368	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	59	theme	outer	257:261	arg1	phase					269:273	the outer water phase	253:273	the outer water phase	253:273	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	59	theme	outer	257:261	arg1	w2					276:277	w2	276:277	w2	276:277	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	60	dep	thickeners	210:219	arg1	thickeners					210:219	thickeners	210:219	thickeners (xanthan and pectin)	210:240	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	60	dep	thickeners	210:219	arg1	pectin					234:239	pectin	234:239	pectin	234:239	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	60	dep	thickeners	210:219	arg1	xanthan					222:228	xanthan	222:228	xanthan	222:228	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	61	theme	phase	1238:1242	arg1	yield					1226:1230	yield	1226:1230	yield	1226:1230	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	61	theme	phase	1238:1242	arg1	size					1217:1220	oil droplet size	1205:1220	oil droplet size	1205:1220	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	1	62	from	effect	119:124	arg1	w1					336:337	w1	336:337	w1	336:337	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	62	from	effect	119:124	arg1	yield					304:308	yield	304:308	yield	304:308	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	62	from	effect	119:124	arg1	size					295:298	oil droplet size	283:298	oil droplet size	283:298	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg2	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg1	phase					269:273	the outer water phase	253:273	the outer water phase	253:273	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg2	Tween					196:200	Tween	196:200	Tween	196:200	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg1	w2					276:277	w2	276:277	w2	276:277	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg2	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	63	attach	present	242:248	arg2	Na-caseinate					178:189	Na-caseinate	178:189	Na-caseinate	178:189	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	64	theme	oil	1205:1207	arg1	droplet					1209:1215	oil droplet	1205:1215	oil droplet size	1205:1220	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	4	65	theme	droplet	727:733	arg1	breakup					735:741	oil droplet breakup	723:741	oil droplet breakup	723:741	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	8	66	theme	w1	1235:1236	arg1	phase					1238:1242	w1 phase	1235:1242	w1 phase of double emulsions	1235:1262	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	67	theme	dispersed	1149:1157	arg1	phase					1159:1163	continuous and dispersed phase	1134:1163	continuous and dispersed phase	1134:1163	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	1	68	theme	double	353:358	arg1	emulsions					360:368	(w1/o/w2) double emulsions	343:368	(w1/o/w2) double emulsions	343:368	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	69	theme	inner	1093:1097	arg1	droplets					1105:1112	inner water droplets	1093:1112	inner water droplets	1093:1112	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	4	70	theme	oil	723:725	arg1	breakup					735:741	oil droplet breakup	723:741	oil droplet breakup	723:741	Upon the addition of thickeners, the increasing viscosity of the outer water phase, w2, facilitated oil droplet breakup.
29580472	8	71	theme	double	1247:1252	arg1	emulsions					1254:1262	double emulsions	1247:1262	double emulsions	1247:1262	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	72	theme	thickener	1185:1193	arg1	type					1177:1180	type	1177:1180	type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener	1047:1193	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	72	theme	thickener	1185:1193	arg1	type					1047:1050	type	1047:1050	type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener	1047:1193	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	1	73	theme	emulsions	360:368	arg1	w1					336:337	w1	336:337	w1	336:337	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	73	theme	emulsions	360:368	arg1	yield					304:308	yield	304:308	yield	304:308	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	73	theme	emulsions	360:368	arg1	size					295:298	oil droplet size	283:298	oil droplet size	283:298	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	74	theme	hydrophilic	1055:1065	arg1	ratio					1125:1129	viscosity ratio	1115:1129	viscosity ratio of continuous and dispersed phase	1115:1163	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	74	theme	hydrophilic	1055:1065	arg1	properties					1079:1088	properties	1079:1088	properties of inner water droplets	1079:1112	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	74	theme	hydrophilic	1055:1065	arg1	emulsifier					1067:1076	hydrophilic emulsifier	1055:1076	hydrophilic emulsifier	1055:1076	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	75	theme	droplet	1209:1215	arg1	size					1217:1220	oil droplet size	1205:1220	oil droplet size	1205:1220	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	76	theme	droplets	1105:1112	arg1	properties					1079:1088	properties	1079:1088	properties of inner water droplets	1079:1112	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	8	76	theme	droplets	1105:1112	arg1	emulsifier					1067:1076	hydrophilic emulsifier	1055:1076	hydrophilic emulsifier	1055:1076	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	6	77	theme	additional	866:875	arg1	decrease					877:884	an additional decrease	863:884	an additional decrease in yield	863:893	When pectin was used as a thickener, in comparison to xanthan, an additional decrease in yield was observed.
29580472	1	78	from	present	242:248	arg1	phase					269:273	the outer water phase	253:273	the outer water phase	253:273	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	78	from	present	242:248	arg1	w2					276:277	w2	276:277	w2	276:277	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	79	theme	present	242:248	arg1	emulsifiers					137:147	various emulsifiers	129:147	various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20)	129:204	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	79	theme	present	242:248	arg1	isolate					163:169	whey protein isolate	150:169	whey protein isolate (WPI)	150:175	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	79	theme	present	242:248	arg1	Na-caseinate					178:189	Na-caseinate	178:189	Na-caseinate	178:189	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	79	theme	present	242:248	arg1	Tween					196:200	Tween	196:200	Tween	196:200	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	1	80	from	phase	269:273	arg1	present					242:248	present	242:248	present	242:248	In this study the effect of various emulsifiers (whey protein isolate (WPI), Na-caseinate, and Tween 20) and thickeners (xanthan and pectin) present in the outer water phase, w2, on oil droplet size and yield of the inner water phase, w1, of (w1/o/w2) double emulsions was investigated.
29580472	8	81	theme	water	1099:1103	arg1	droplets					1105:1112	inner water droplets	1093:1112	inner water droplets	1093:1112	We conclude that type of hydrophilic emulsifier, properties of inner water droplets, viscosity ratio of continuous and dispersed phase, as well as type of thickener influence oil droplet size and yield of w1 phase of double emulsions.
29580472	6	82	from	decrease	877:884	arg1	yield					889:893	yield	889:893	yield	889:893	When pectin was used as a thickener, in comparison to xanthan, an additional decrease in yield was observed.
30790408	7	0	theme	maltodextrin	1161:1172	arg1	application					1146:1156	the application	1142:1156	the application of maltodextrin	1142:1172	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	7	0	theme	maltodextrin	1161:1172	arg1	choice					1179:1184	a choice	1177:1184	a choice to protect probiotic L. plantarum strains against the bile salt stress	1177:1255	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	4	1	theme	survival	665:672	arg1	rate					674:677	the highest survival rate	653:677	the highest survival rate	653:677	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	1	2	theme	bile	163:166	arg1	tolerance					168:176	bile tolerance	163:176	bile tolerance	163:176	AIMS To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp-115.
30790408	4	3	theme	highest	657:663	arg1	rate					674:677	the highest survival rate	653:677	the highest survival rate	653:677	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	5	4	theme	gene	962:965	arg1	expression					926:935	the expression	922:935	the expression of a bile salt hydrolase gene (pva3)	922:972	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	0	5	from	Enhancement	0:10	arg1	Lp-115					90:95	Lactobacillus plantarum Lp-115	66:95	Lactobacillus plantarum Lp-115	66:95	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	2	6	theme	viable	298:303	arg1	cells					305:309	viable cells	298:309	viable cells	298:309	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	6	7	theme	maltodextrin	1079:1090	arg1	mechanism					1066:1074	the mechanism	1062:1074	the mechanism of maltodextrin enhancing the bile tolerance	1062:1119	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	4	8	theme	bile	701:704	arg1	concentration					706:718	the bile concentration	697:718	the bile concentration	697:718	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	4	8	theme	bile	701:704	arg1	%					726:726	0.3%	723:726	0.3%	723:726	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	3	9	theme	MRSB	522:525	arg1	broth					527:531	MRSB broth	522:531	MRSB broth with maltodextrin	522:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	1	10	theme	way	138:140	arg1	maltodextrin					142:153	way maltodextrin	138:153	way maltodextrin	138:153	AIMS To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp-115.
30790408	2	11	from	tolerance	442:450	arg1	Lp-115					463:468	Lp-115	463:468	Lp-115	463:468	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	3	12	theme	Lp-115	503:508	arg1	times					561:565	three times	555:565	three times higher than the control value	555:595	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	3	12	theme	Lp-115	503:508	arg1	value					481:485	The OD600 value	471:485	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin	471:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	4	13	theme	4·0	634:636	arg1	%					637:637	%	637:637	%	637:637	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	6	14	dep	SIGNIFICANCE	989:1000	arg1	This					1026:1029	This	1026:1029	This	1026:1029	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	1	15	theme	Lactobacillus	181:193	arg1	Lp-115					205:210	Lactobacillus plantarum Lp-115	181:210	Lactobacillus plantarum Lp-115	181:210	AIMS To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp-115.
30790408	3	16	with	broth	527:531	arg1	maltodextrin					538:549	maltodextrin	538:549	maltodextrin	538:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	6	17	theme	bile	1106:1109	arg1	tolerance					1111:1119	the bile tolerance	1102:1119	the bile tolerance	1102:1119	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	7	18	theme	L.	1207:1208	arg1	strains					1220:1226	probiotic L. plantarum strains	1197:1226	probiotic L. plantarum strains	1197:1226	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	2	19	theme	pour	318:321	arg1	method					329:334	the pour plate method	314:334	the pour plate method	314:334	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	1	20	theme	plantarum	195:203	arg1	Lp-115					205:210	Lactobacillus plantarum Lp-115	181:210	Lactobacillus plantarum Lp-115	181:210	AIMS To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp-115.
30790408	5	21	theme	membrane	902:909	arg1	composition					883:893	the fatty acid composition	868:893	the fatty acid composition of the membrane	868:909	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	7	22	theme	salt	1245:1248	arg1	stress					1250:1255	the bile salt stress	1236:1255	the bile salt stress	1236:1255	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	4	23	theme	%	637:637	arg1	maltodextrin					639:650	4·0% maltodextrin	634:650	4·0% maltodextrin	634:650	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	0	24	theme	resistance	20:29	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.	0:96	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	7	25	theme	probiotic	1197:1205	arg1	strains					1220:1226	probiotic L. plantarum strains	1197:1226	probiotic L. plantarum strains	1197:1226	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	0	26	theme	bile	15:18	arg1	resistance					20:29	bile resistance	15:29	bile resistance	15:29	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	5	27	dep	CONCLUSIONS	729:739	arg1	enhance					833:839	enhance	833:839	enhance	833:839	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	5	27	dep	CONCLUSIONS	729:739	arg1	exhibited					766:774	exhibited	766:774	exhibited a significant improvement of bile tolerance	766:818	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	2	28	theme	cells	305:309	arg1	numbers					287:293	the numbers	283:293	the numbers of viable cells	283:309	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	5	29	theme	bile	805:808	arg1	tolerance					810:818	bile tolerance	805:818	bile tolerance	805:818	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	0	30	theme	maltodextrin	34:45	arg1	supplementation					47:61	maltodextrin supplementation	34:61	maltodextrin supplementation	34:61	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	5	31	theme	acid	878:881	arg1	composition					883:893	the fatty acid composition	868:893	the fatty acid composition of the membrane	868:909	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	7	32	theme	bile	1240:1243	arg1	stress					1250:1255	the bile salt stress	1236:1255	the bile salt stress	1236:1255	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	3	33	theme	higher	567:572	arg1	times					561:565	three times	555:565	three times higher than the control value	555:595	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	3	33	theme	higher	567:572	arg1	value					481:485	The OD600 value	471:485	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin	471:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	5	34	theme	cell	841:844	arg1	hydrophobicity					846:859	cell hydrophobicity	841:859	cell hydrophobicity	841:859	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	5	35	theme	tolerance	810:818	arg1	improvement					790:800	a significant improvement	776:800	a significant improvement of bile tolerance	776:818	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	2	36	theme	bile	455:458	arg1	tolerance					442:450	the tolerance	438:450	the tolerance of bile in Lp-115	438:468	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	5	37	theme	significant	778:788	arg1	improvement					790:800	a significant improvement	776:800	a significant improvement of bile tolerance	776:818	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	2	38	theme	strain	396:401	arg1	growth					403:408	the strain growth	392:408	the strain growth	392:408	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	5	39	theme	fatty	872:876	arg1	composition					883:893	the fatty acid composition	868:893	the fatty acid composition of the membrane	868:909	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	4	40	with	medium	622:627	arg1	maltodextrin					639:650	4·0% maltodextrin	634:650	4·0% maltodextrin	634:650	After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.
30790408	3	41	theme	OD600	475:479	arg1	times					561:565	three times	555:565	three times higher than the control value	555:595	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	3	41	theme	OD600	475:479	arg1	value					481:485	The OD600 value	471:485	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin	471:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	3	42	theme	control	583:589	arg1	value					591:595	the control value	579:595	the control value	579:595	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	5	43	theme	bile	942:945	arg1	pva3					968:971	pva3	968:971	pva3	968:971	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	5	43	theme	bile	942:945	arg1	gene					962:965	bile salt hydrolase gene	942:965	a bile salt hydrolase gene (pva3)	940:972	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	7	44	theme	plantarum	1210:1218	arg1	strains					1220:1226	probiotic L. plantarum strains	1197:1226	probiotic L. plantarum strains	1197:1226	This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.
30790408	5	45	theme	salt	947:950	arg1	pva3					968:971	pva3	968:971	pva3	968:971	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	5	45	theme	salt	947:950	arg1	gene					962:965	bile salt hydrolase gene	942:965	a bile salt hydrolase gene (pva3)	940:972	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	2	46	theme	plate	323:327	arg1	method					329:334	the pour plate method	314:334	the pour plate method	314:334	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	0	47	theme	plantarum	80:88	arg1	Lp-115					90:95	Lactobacillus plantarum Lp-115	66:95	Lactobacillus plantarum Lp-115	66:95	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	6	48	theme	first	1038:1042	arg1	report					1044:1049	the first report	1034:1049	the first report concerning the mechanism of maltodextrin enhancing the bile tolerance	1034:1119	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	6	48	theme	first	1038:1042	arg1	SIGNIFICANCE					989:1000	SIGNIFICANCE	989:1000	SIGNIFICANCE	989:1000	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	6	48	theme	first	1038:1042	arg1	IMPACT					1006:1011	IMPACT	1006:1011	IMPACT	1006:1011	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	5	49	theme	hydrolase	952:960	arg1	pva3					968:971	pva3	968:971	pva3	968:971	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	5	49	theme	hydrolase	952:960	arg1	gene					962:965	bile salt hydrolase gene	942:965	a bile salt hydrolase gene (pva3)	940:972	CONCLUSIONS In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.
30790408	3	50	theme	L.	490:491	arg1	Lp-115					503:508	L. plantarum Lp-115	490:508	L. plantarum Lp-115 cultured in MRSB broth with maltodextrin	490:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30790408	6	51	theme	STUDY	1020:1024	arg1	IMPACT					1006:1011	IMPACT	1006:1011	IMPACT	1006:1011	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	6	51	theme	STUDY	1020:1024	arg1	SIGNIFICANCE					989:1000	SIGNIFICANCE	989:1000	SIGNIFICANCE	989:1000	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	6	51	theme	STUDY	1020:1024	arg1	report					1044:1049	the first report	1034:1049	the first report concerning the mechanism of maltodextrin enhancing the bile tolerance	1034:1119	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance.
30790408	0	52	theme	Lactobacillus	66:78	arg1	Lp-115					90:95	Lactobacillus plantarum Lp-115	66:95	Lactobacillus plantarum Lp-115	66:95	Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.
30790408	2	53	theme	OD600	258:262	arg1	value					264:268	the OD600 value	254:268	the OD600 value	254:268	METHODS AND RESULTS Based on determining the OD600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115.
30790408	3	54	theme	plantarum	493:501	arg1	Lp-115					503:508	L. plantarum Lp-115	490:508	L. plantarum Lp-115 cultured in MRSB broth with maltodextrin	490:549	The OD600 value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value.
30680582	2	0	theme	sulfate	607:613	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	3	1	theme	Cartilaginous	616:628	arg1	tissues					630:636	Cartilaginous tissues	616:636	Cartilaginous tissues	616:636	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	4	2	theme	High	821:824	arg1	degrees					845:851	High O- and N-sulfation degrees	821:851	High O- and N-sulfation degrees of HS	821:857	High O- and N-sulfation degrees of HS correspond to high ossification.
30680582	1	3	dep	stages	234:239	arg1	stages					234:239	the different growing stages	212:239	the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	212:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	3	dep	stages	234:239	arg1	Anshi					254:258	Anshi	254:258	Anshi	254:258	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	3	dep	stages	234:239	arg1	Santajo					265:271	Santajo	265:271	Santajo	265:271	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	2	4	theme	sulfate	547:553	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	3	5	with	concentrations	662:675	arg1	ratio					698:702	an almost equal ratio	682:702	an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	682:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	2	6	theme	hyaluronan	561:570	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	1	7	theme	different	216:224	arg1	stages					234:239	the different growing stages	212:239	the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	212:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	7	theme	different	216:224	arg1	Anshi					254:258	Anshi	254:258	Anshi	254:258	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	7	theme	different	216:224	arg1	Santajo					265:271	Santajo	265:271	Santajo	265:271	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	8	theme	wild	286:289	arg1	deer					291:294	bred and wild deer	277:294	bred and wild deer	277:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	5	9	from	changes	913:919	arg1	HS					969:970	HS	969:970	HS	969:970	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	5	9	from	changes	913:919	arg1	CS-DS					959:963	CS-DS	959:963	CS-DS	959:963	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	5	9	from	changes	913:919	arg1	CS-DS					924:928	CS-DS	924:928	CS-DS	924:928	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	1	10	theme	growing	226:232	arg1	stages					234:239	the different growing stages	212:239	the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	212:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	10	theme	growing	226:232	arg1	Anshi					254:258	Anshi	254:258	Anshi	254:258	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	10	theme	growing	226:232	arg1	Santajo					265:271	Santajo	265:271	Santajo	265:271	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	11	theme	deer	291:294	arg1	stages					234:239	the different growing stages	212:239	the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	212:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	11	theme	deer	291:294	arg1	Anshi					254:258	Anshi	254:258	Anshi	254:258	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	11	theme	deer	291:294	arg1	Santajo					265:271	Santajo	265:271	Santajo	265:271	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	3	12	dep	occupied	767:774	arg1	ossified					776:783	ossified	776:783	CS-DS predominantly occupied ossified tissues	747:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	3	13	theme	6-sulfates	714:723	arg1	ratio					698:702	an almost equal ratio	682:702	an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	682:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	0	14	theme	Compositional	0:12	arg1	analysis					14:21	Compositional analysis	0:21	Compositional analysis of the glycosaminoglycan family in velvet	0:63	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	5	15	theme	deer	1023:1026	arg1	antlers					1028:1034	deer antlers	1023:1034	deer antlers	1023:1034	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	1	16	from	stages	234:239	arg1	antlers					172:178	the velvet antlers	161:178	the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	161:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	3	17	theme	low	801:803	arg1	concentrations					805:818	low concentrations	801:818	low concentrations	801:818	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	0	18	from	analysis	14:21	arg1	velvet					58:63	velvet	58:63	velvet	58:63	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	2	19	theme	dermatan	538:545	arg1	sulfate					547:553	dermatan sulfate	538:553	dermatan sulfate (DS)	538:558	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	2	19	theme	dermatan	538:545	arg1	DS					556:557	DS	556:557	DS	556:557	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	2	20	theme	sulfate	581:587	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	3	21	contain	contained	638:646	arg2	tissues					630:636	Cartilaginous tissues	616:636	Cartilaginous tissues	616:636	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	3	21	contain	contained	638:646	arg1	concentrations					805:818	low concentrations	801:818	low concentrations	801:818	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	3	21	contain	contained	638:646	arg1	concentrations					662:675	high concentrations	657:675	high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	657:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	3	21	contain	contained	638:646	arg2	CS-DS					648:652	CS-DS	648:652	CS-DS	648:652	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	3	21	contain	contained	638:646	arg1	tissues					630:636	Cartilaginous tissues	616:636	Cartilaginous tissues	616:636	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	1	22	theme	velvet	165:170	arg1	antlers					172:178	the velvet antlers	161:178	the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	161:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	3	23	theme	high	657:660	arg1	concentrations					662:675	high concentrations	657:675	high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	657:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	0	24	theme	different	105:113	arg1	stages					123:128	different growing stages	105:128	different growing stages	105:128	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	2	25	theme	heparan	573:579	arg1	sulfate					581:587	heparan sulfate	573:587	heparan sulfate (HS)	573:592	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	2	25	theme	heparan	573:579	arg1	HS					590:591	HS	590:591	HS	590:591	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	5	26	from	changes	948:954	arg1	HS					969:970	HS	969:970	HS	969:970	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	5	26	from	changes	948:954	arg1	CS-DS					959:963	CS-DS	959:963	CS-DS	959:963	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	5	26	from	changes	948:954	arg1	CS-DS					924:928	CS-DS	924:928	CS-DS	924:928	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	2	27	theme	corresponding	471:483	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	0	28	theme	family	48:53	arg1	analysis					14:21	Compositional analysis	0:21	Compositional analysis of the glycosaminoglycan family in velvet	0:63	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	4	29	theme	N-sulfation	833:843	arg1	degrees					845:851	High O- and N-sulfation degrees	821:851	High O- and N-sulfation degrees of HS	821:857	High O- and N-sulfation degrees of HS correspond to high ossification.
30680582	1	30	theme	bred	277:280	arg1	deer					291:294	bred and wild deer	277:294	bred and wild deer	277:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	0	31	theme	glycosaminoglycan	30:46	arg1	family					48:53	the glycosaminoglycan family	26:53	the glycosaminoglycan family	26:53	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	0	32	theme	growing	115:121	arg1	stages					123:128	different growing stages	105:128	different growing stages	105:128	Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
30680582	2	33	theme	keratan	599:605	arg1	sulfate					607:613	keratan sulfate	599:613	keratan sulfate	599:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	1	34	theme	deer	188:191	arg1	antlers					172:178	the velvet antlers	161:178	the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	161:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	2	35	theme	chondroitinase	396:409	arg1	ABC					411:413	chondroitinase ABC	396:413	chondroitinase ABC	396:413	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	2	36	theme	sulfate	524:530	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	3	37	dep	4-	707:708	arg1	CS-DS					747:751	CS-DS	747:751	CS-DS predominantly occupied ossified tissues	747:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	5	38	theme	compositional	934:946	arg1	changes					948:954	compositional changes	934:954	compositional changes in CS-DS and HS	934:970	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	2	39	theme	chondroitin	512:522	arg1	CS					533:534	CS	533:534	CS	533:534	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	2	39	theme	chondroitin	512:522	arg1	sulfate					524:530	chondroitin sulfate	512:530	chondroitin sulfate (CS)	512:535	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	1	40	theme	sulfation	339:347	arg1	patterns					349:356	sulfation patterns	339:356	sulfation patterns	339:356	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	41	from	antlers	172:178	arg1	Glycosaminoglycans					131:148	Glycosaminoglycans	131:148	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	131:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	41	from	antlers	172:178	arg1	stages					234:239	the different growing stages	212:239	the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer	212:294	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	41	from	antlers	172:178	arg1	Santajo					265:271	Santajo	265:271	Santajo	265:271	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	41	from	antlers	172:178	arg1	Anshi					254:258	Anshi	254:258	Anshi	254:258	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	1	41	from	antlers	172:178	arg1	GAG					151:153	GAG	151:153	GAG	151:153	Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed.
30680582	2	42	theme	repeating	485:493	arg1	disaccharides					495:507	the corresponding repeating disaccharides	467:507	the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate	467:613	GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate.
30680582	5	43	theme	Dynamic	892:898	arg1	changes					913:919	Dynamic quantitative changes	892:919	Dynamic quantitative changes in CS-DS	892:928	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	3	44	dep	CS-DS	747:751	arg1	occupied					767:774	occupied	767:774	CS-DS predominantly occupied ossified tissues	747:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	5	45	theme	quantitative	900:911	arg1	changes					913:919	Dynamic quantitative changes	892:919	Dynamic quantitative changes in CS-DS	892:928	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	3	46	theme	4-	707:708	arg1	ratio					698:702	an almost equal ratio	682:702	an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	682:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	4	47	theme	high	873:876	arg1	ossification					878:889	high ossification	873:889	high ossification	873:889	High O- and N-sulfation degrees of HS correspond to high ossification.
30680582	4	48	theme	O-	826:827	arg1	degrees					845:851	High O- and N-sulfation degrees	821:851	High O- and N-sulfation degrees of HS	821:857	High O- and N-sulfation degrees of HS correspond to high ossification.
30680582	3	49	theme	equal	692:696	arg1	ratio					698:702	an almost equal ratio	682:702	an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues	682:791	Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations.
30680582	5	50	theme	antlers	1028:1034	arg1	mineralization					1005:1018	the mineralization	1001:1018	the mineralization of deer antlers	1001:1034	Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.
30680582	4	51	theme	HS	856:857	arg1	degrees					845:851	High O- and N-sulfation degrees	821:851	High O- and N-sulfation degrees of HS	821:857	High O- and N-sulfation degrees of HS correspond to high ossification.
31426950	7	0	theme	interfacial	1041:1051	arg1	adhesion					1053:1060	a poor interfacial adhesion	1034:1060	a poor interfacial adhesion between the pea starch and proteins	1034:1096	This result suggested that there was a poor interfacial adhesion between the pea starch and proteins.
31426950	7	1	theme	poor	1036:1039	arg1	adhesion					1053:1060	a poor interfacial adhesion	1034:1060	a poor interfacial adhesion between the pea starch and proteins	1034:1096	This result suggested that there was a poor interfacial adhesion between the pea starch and proteins.
31426950	1	2	theme	pea	175:177	arg1	blend					194:198	pea flour and pea starch-protein blend	161:198	blend	194:198	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	3	theme	low	275:277	arg1	temperature					263:273	a temperature	261:273	a temperature low enough to avoid expansion	261:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	3	4	theme	total	513:517	arg1	aggregates					489:498	protein aggregates	481:498	protein aggregates (D50)	481:504	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	4	theme	total	513:517	arg1	perimeter					519:527	their total perimeter	507:527	their total perimeter	507:527	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	5	5	theme	blend	843:847	arg1	composites					849:858	the blend composites	839:858	the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm)	839:912	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	1	6	theme	Starch-legume	98:110	arg1	composites					120:129	Starch-legume protein composites	98:129	Starch-legume protein composites	98:129	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	4	7	theme	mechanical	608:617	arg1	properties					619:628	The mechanical properties	604:628	The mechanical properties of composites	604:642	The mechanical properties of composites were determined by a three-point bending test.
31426950	3	8	used	used	443:446	arg2	analysis					430:437	Image analysis	424:437	Image analysis	424:437	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	5	9	theme	higher	722:727	arg1	index					739:743	a higher interface index Ii	720:746	a higher interface index Ii (1.8-3.1)	720:756	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	9	theme	higher	722:727	arg1	1.8-3.1					749:755	1.8-3.1	749:755	1.8-3.1	749:755	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	3	10	theme	protein	481:487	arg1	D50					501:503	D50	501:503	D50	501:503	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	10	theme	protein	481:487	arg1	aggregates					489:498	protein aggregates	481:498	protein aggregates (D50)	481:504	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	10	theme	protein	481:487	arg1	surface					533:539	surface	533:539	surface	533:539	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	10	theme	protein	481:487	arg1	perimeter					519:527	their total perimeter	507:527	their total perimeter	507:527	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	5	11	theme	lower	871:875	arg1	1-1.1					881:885	1-1.1	881:885	1-1.1	881:885	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	11	theme	lower	871:875	arg1	Ii					877:878	a lower Ii	869:878	a lower Ii (1-1.1)	869:886	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	1	12	theme	starch-protein	179:192	arg1	blend					194:198	pea flour and pea starch-protein blend	161:198	blend	194:198	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	3	13	theme	Image	424:428	arg1	analysis					430:437	Image analysis	424:437	Image analysis	424:437	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	1	14	from	extrusion	148:156	arg1	100-2000 kJ/kg					241:254	100-2000 kJ/kg	241:254	100-2000 kJ/kg	241:254	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	14	from	extrusion	148:156	arg1	energies					231:238	various specific mechanical energies	203:238	various specific mechanical energies (100-2000 kJ/kg)	203:255	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	14	from	extrusion	148:156	arg1	temperature					263:273	a temperature	261:273	a temperature low enough to avoid expansion	261:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	6	15	theme	rupture	935:941	arg1	stress					943:948	rupture stress	935:948	rupture stress	935:948	For both materials, rupture stress and strain were negatively correlated with Ii.
31426950	1	16	theme	blend	194:198	arg1	extrusion					148:156	extrusion	148:156	extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion	148:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	5	17	theme	particle	776:783	arg1	width					785:789	lower median particle width D50	763:793	lower median particle width D50 (8-18 μm)	763:803	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	17	theme	particle	776:783	arg1	8-18 μm					796:802	8-18 μm	796:802	8-18 μm	796:802	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	18	contain	had	716:718	arg2	1.8-3.1					749:755	1.8-3.1	749:755	1.8-3.1	749:755	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	18	contain	had	716:718	arg2	index					739:743	a higher interface index Ii	720:746	a higher interface index Ii (1.8-3.1)	720:756	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	18	contain	had	716:718	arg1	composites					705:714	The pea flour composites	691:714	The pea flour composites	691:714	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	2	19	theme	composites	330:339	arg1	morphology					310:319	The morphology	306:319	The morphology of these composites	306:339	The morphology of these composites displayed protein aggregates dispersed in a starch matrix, revealed by microscopy.
31426950	4	20	theme	composites	633:642	arg1	properties					619:628	The mechanical properties	604:628	The mechanical properties of composites	604:642	The mechanical properties of composites were determined by a three-point bending test.
31426950	5	21	theme	flour	699:703	arg1	composites					705:714	The pea flour composites	691:714	The pea flour composites	691:714	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	1	22	theme	protein	112:118	arg1	composites					120:129	Starch-legume protein composites	98:129	Starch-legume protein composites	98:129	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	23	theme	various	203:209	arg1	100-2000 kJ/kg					241:254	100-2000 kJ/kg	241:254	100-2000 kJ/kg	241:254	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	23	theme	various	203:209	arg1	energies					231:238	various specific mechanical energies	203:238	various specific mechanical energies (100-2000 kJ/kg)	203:255	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	7	24	theme	pea	1074:1076	arg1	starch					1078:1083	the pea starch	1070:1083	the pea starch	1070:1083	This result suggested that there was a poor interfacial adhesion between the pea starch and proteins.
31426950	1	25	theme	specific	211:218	arg1	100-2000 kJ/kg					241:254	100-2000 kJ/kg	241:254	100-2000 kJ/kg	241:254	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	25	theme	specific	211:218	arg1	energies					231:238	various specific mechanical energies	203:238	various specific mechanical energies (100-2000 kJ/kg)	203:255	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	0	26	theme	mechanical	15:24	arg1	behaviour					26:34	mechanical behaviour	15:34	mechanical behaviour	15:34	Morphology and mechanical behaviour of pea-based starch-protein composites obtained by extrusion.
31426950	5	27	theme	pea	695:697	arg1	composites					705:714	The pea flour composites	691:714	The pea flour composites	691:714	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	1	28	theme	mechanical	220:229	arg1	100-2000 kJ/kg					241:254	100-2000 kJ/kg	241:254	100-2000 kJ/kg	241:254	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	28	theme	mechanical	220:229	arg1	energies					231:238	various specific mechanical energies	203:238	various specific mechanical energies (100-2000 kJ/kg)	203:255	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	0	29	theme	pea-based	39:47	arg1	composites					64:73	pea-based starch-protein composites	39:73	pea-based starch-protein composites	39:73	Morphology and mechanical behaviour of pea-based starch-protein composites obtained by extrusion.
31426950	1	30	from	temperature	263:273	arg1	extrusion					148:156	extrusion	148:156	extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion	148:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	30	from	temperature	263:273	arg1	flour					165:169	pea flour and pea starch-protein blend	161:198	flour	165:169	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	30	from	temperature	263:273	arg1	blend					194:198	pea flour and pea starch-protein blend	161:198	blend	194:198	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	3	31	theme	median	465:470	arg1	width					472:476	the median width	461:476	the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived	461:601	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	32	theme	interface	570:578	arg1	Ii					587:588	Ii	587:588	Ii	587:588	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	32	theme	interface	570:578	arg1	index					580:584	a protein/starch interface index	553:584	a protein/starch interface index (Ii)	553:589	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	4	33	theme	bending	677:683	arg1	test					685:688	a three-point bending test	663:688	a three-point bending test	663:688	The mechanical properties of composites were determined by a three-point bending test.
31426950	1	34	from	energies	231:238	arg1	extrusion					148:156	extrusion	148:156	extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion	148:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	34	from	energies	231:238	arg1	flour					165:169	pea flour and pea starch-protein blend	161:198	flour	165:169	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	34	from	energies	231:238	arg1	blend					194:198	pea flour and pea starch-protein blend	161:198	blend	194:198	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	5	35	theme	interface	729:737	arg1	index					739:743	a higher interface index Ii	720:746	a higher interface index Ii (1.8-3.1)	720:756	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	35	theme	interface	729:737	arg1	1.8-3.1					749:755	1.8-3.1	749:755	1.8-3.1	749:755	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	0	36	theme	composites	64:73	arg1	Morphology					0:9	Morphology	0:9	Morphology	0:9	Morphology and mechanical behaviour of pea-based starch-protein composites obtained by extrusion.
31426950	0	36	theme	composites	64:73	arg1	behaviour					26:34	mechanical behaviour	15:34	mechanical behaviour	15:34	Morphology and mechanical behaviour of pea-based starch-protein composites obtained by extrusion.
31426950	5	37	theme	median	769:774	arg1	width					785:789	lower median particle width D50	763:793	lower median particle width D50 (8-18 μm)	763:803	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	37	theme	median	769:774	arg1	8-18 μm					796:802	8-18 μm	796:802	8-18 μm	796:802	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	0	38	theme	starch-protein	49:62	arg1	composites					64:73	pea-based starch-protein composites	39:73	pea-based starch-protein composites	39:73	Morphology and mechanical behaviour of pea-based starch-protein composites obtained by extrusion.
31426950	3	39	theme	protein/starch	555:568	arg1	Ii					587:588	Ii	587:588	Ii	587:588	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	39	theme	protein/starch	555:568	arg1	index					580:584	a protein/starch interface index	553:584	a protein/starch interface index (Ii)	553:589	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	4	40	theme	three-point	665:675	arg1	test					685:688	a three-point bending test	663:688	a three-point bending test	663:688	The mechanical properties of composites were determined by a three-point bending test.
31426950	2	41	theme	protein	351:357	arg1	aggregates					359:368	protein aggregates	351:368	protein aggregates	351:368	The morphology of these composites displayed protein aggregates dispersed in a starch matrix, revealed by microscopy.
31426950	5	42	contain	had	865:867	arg1	composites					849:858	the blend composites	839:858	the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm)	839:912	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	42	contain	had	865:867	arg2	Ii					877:878	a lower Ii	869:878	a lower Ii (1-1.1)	869:886	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	42	contain	had	865:867	arg2	D50					899:901	higher D50	892:901	higher D50 (22-31 μm)	892:912	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	42	contain	had	865:867	arg2	22-31 μm					904:911	22-31 μm	904:911	22-31 μm	904:911	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	42	contain	had	865:867	arg2	1-1.1					881:885	1-1.1	881:885	1-1.1	881:885	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	43	theme	higher	892:897	arg1	D50					899:901	higher D50	892:901	higher D50 (22-31 μm)	892:912	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	43	theme	higher	892:897	arg1	22-31 μm					904:911	22-31 μm	904:911	22-31 μm	904:911	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	1	44	theme	flour	165:169	arg1	extrusion					148:156	extrusion	148:156	extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion	148:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	3	45	attach	derived	595:601	arg1	perimeter					519:527	their total perimeter	507:527	their total perimeter	507:527	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	45	attach	derived	595:601	arg1	aggregates					489:498	protein aggregates	481:498	protein aggregates (D50)	481:504	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	45	attach	derived	595:601	arg1	D50					501:503	D50	501:503	D50	501:503	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	45	attach	derived	595:601	arg2	index					580:584	a protein/starch interface index	553:584	a protein/starch interface index (Ii)	553:589	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	45	attach	derived	595:601	arg1	surface					533:539	surface	533:539	surface	533:539	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	45	attach	derived	595:601	arg2	Ii					587:588	Ii	587:588	Ii	587:588	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	3	46	theme	aggregates	489:498	arg1	width					472:476	the median width	461:476	the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived	461:601	Image analysis was used to determine the median width of protein aggregates (D50), their total perimeter and surface, from which a protein/starch interface index (Ii) was derived.
31426950	1	47	dep	low	275:277	arg1	avoid					289:293	avoid	289:293	to avoid expansion	286:303	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	1	48	theme	pea	161:163	arg1	flour					165:169	pea flour and pea starch-protein blend	161:198	flour	165:169	Starch-legume protein composites were obtained by extrusion of pea flour and pea starch-protein blend at various specific mechanical energies (100-2000 kJ/kg) and a temperature low enough to avoid expansion.
31426950	5	49	theme	brittle	816:822	arg1	behaviour					824:832	a more brittle behaviour	809:832	a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm)	809:912	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	50	theme	lower	763:767	arg1	width					785:789	lower median particle width D50	763:793	lower median particle width D50 (8-18 μm)	763:803	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	5	50	theme	lower	763:767	arg1	8-18 μm					796:802	8-18 μm	796:802	8-18 μm	796:802	The pea flour composites had a higher interface index Ii (1.8-3.1) with lower median particle width D50 (8-18 μm) and a more brittle behaviour than the blend composites that had a lower Ii (1-1.1) and higher D50 (22-31 μm).
31426950	2	51	theme	starch	385:390	arg1	matrix					392:397	a starch matrix	383:397	a starch matrix	383:397	The morphology of these composites displayed protein aggregates dispersed in a starch matrix, revealed by microscopy.
30458193	2	0	with	polymer	230:236	arg1	variety					245:251	a variety	243:251	a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	243:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	0	with	polymer	230:236	arg1	applications					256:267	applications	256:267	applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	256:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	9	1	theme	RAC	1455:1457	arg1	aptamer					1459:1465	The RAC aptamer	1451:1465	The RAC aptamer	1451:1465	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	4	2	dep	nanocomposite	731:743	arg1	modified					745:752	modified	745:752	modified by gold nanostructures (Au NSs)	745:784	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	2	dep	nanocomposite	731:743	arg1	used					790:793	used	790:793	used for quantification of RAC	790:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	6	3	theme	surface	1138:1144	arg1	area					1146:1149	a high surface area	1131:1149	a high surface area to immobilize a high amount of the aptamer	1131:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	4	4	theme	graphene	688:695	arg1	CS					727:728	CS	727:728	CS	727:728	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	4	theme	graphene	688:695	arg1	GQDs					711:714	GQDs	711:714	GQDs	711:714	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	4	theme	graphene	688:695	arg1	dots					705:708	thiolated graphene quantum dots	678:708	thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC	678:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	10	5	theme	based	1576:1580	arg1	assay					1582:1586	the EC aptamer based assay	1561:1586	the EC aptamer based assay	1561:1586	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	3	6	theme	RAC	571:573	arg1	aptamer					576:582	ractopamine (RAC) aptamer	558:582	ractopamine (RAC) aptamer	558:582	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	5	7	theme	various	867:873	arg1	sizes					875:879	various sizes	867:879	various sizes from zero-dimensional nanoparticles	867:915	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	10	8	theme	electrochemical	1614:1628	arg1	techniques					1630:1639	some electrochemical techniques	1609:1639	some electrochemical techniques	1609:1639	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	9	9	theme	Au	1487:1488	arg1	NSs					1490:1492	the Au NSs	1483:1492	the Au NSs of the composite membrane	1483:1518	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	11	10	theme	RAC	1835:1837	arg1	concentration					1839:1851	RAC concentration	1835:1851	RAC concentration	1835:1851	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	2	11	theme	antibacterial	310:322	arg1	activity					324:331	antibacterial activity	310:331	antibacterial activity	310:331	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	8	12	theme	special	1304:1310	arg1	media					1327:1331	A special immobilization media	1302:1331	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA)	1302:1378	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	5	13	theme	nanostructures	847:860	arg1	shapes					832:837	Different shapes	822:837	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures	822:939	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	3	14	mod	modified	497:504	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	3	14	mod	modified	497:504	arg3	nanoparticle					514:525	gold nanoparticle	509:525	gold nanoparticle	509:525	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	6	15	theme	new	1074:1076	arg1	transducer					1078:1087	a new transducer	1072:1087	a new transducer	1072:1087	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	7	16	theme	ultrasensitive	1255:1268	arg1	detection					1270:1278	ultrasensitive detection	1255:1278	ultrasensitive detection of RAC	1255:1285	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	5	17	theme	Different	822:830	arg1	shapes					832:837	Different shapes	822:837	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures	822:939	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	1	18	theme	biomedical	169:178	arg1	number					135:140	a number	133:140	a number of commercial and possible biomedical uses	133:183	Chitosan has a number of commercial and possible biomedical uses.
30458193	4	19	theme	-chitosan	716:724	arg1	CS					727:728	CS	727:728	CS	727:728	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	19	theme	-chitosan	716:724	arg1	GQDs					711:714	GQDs	711:714	GQDs	711:714	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	19	theme	-chitosan	716:724	arg1	dots					705:708	thiolated graphene quantum dots	678:708	thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC	678:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	0	20	theme	aptamer	96:102	arg1	immobilization					104:117	aptamer immobilization	96:117	aptamer immobilization	96:117	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	5	21	theme	spherical	920:928	arg1	structures					930:939	spherical structures	920:939	spherical structures	920:939	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	4	22	theme	Au	778:779	arg1	nanostructures					762:775	gold nanostructures	757:775	gold nanostructures (Au NSs)	757:784	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	22	theme	Au	778:779	arg1	NSs					781:783	Au NSs	778:783	Au NSs	778:783	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	23	theme	gold	757:760	arg1	nanostructures					762:775	gold nanostructures	757:775	gold nanostructures (Au NSs)	757:784	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	23	theme	gold	757:760	arg1	NSs					781:783	Au NSs	778:783	Au NSs	778:783	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	11	24	theme	wide	1813:1816	arg1	range					1826:1830	a wide dynamic range	1811:1830	a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM	1811:1878	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	3	25	theme	aptamer	576:582	arg1	immobilization					540:553	immobilization	540:553	immobilization of ractopamine (RAC) aptamer	540:582	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	8	26	used	utilized	1384:1391	arg2	media					1327:1331	A special immobilization media	1302:1331	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA)	1302:1378	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	7	27	theme	electrochemical	1219:1233	arg1	apta-assay					1240:1249	a label free electrochemical (EC) apta-assay	1206:1249	a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC	1206:1285	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	7	28	theme	label	1208:1212	arg1	apta-assay					1240:1249	a label free electrochemical (EC) apta-assay	1206:1249	a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC	1206:1285	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	5	29	theme	template-assistant	967:984	arg1	method					1010:1015	one-step template-assistant green electrodeposition method	958:1015	one-step template-assistant green electrodeposition method	958:1015	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	0	30	theme	graphene	32:39	arg1	polysaccharide					73:86	a biocompatible polysaccharide	57:86	a biocompatible polysaccharide towards aptamer immobilization	57:117	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	0	30	theme	graphene	32:39	arg1	dots					49:52	chitosan/thiolated graphene quantum dots	13:52	chitosan/thiolated graphene quantum dots	13:52	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	5	31	theme	electrodeposition	992:1008	arg1	method					1010:1015	one-step template-assistant green electrodeposition method	958:1015	one-step template-assistant green electrodeposition method	958:1015	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	6	32	theme	glassy	1094:1099	arg1	surface					1108:1114	a glassy carbon surface	1092:1114	a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer	1092:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	3	33	theme	graphene	438:445	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	11	34	theme	RAC	1745:1747	arg1	concentrations					1749:1762	RAC concentrations	1745:1762	RAC concentrations	1745:1762	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	4	35	theme	RAC	817:819	arg1	quantification					799:812	quantification	799:812	quantification of RAC	799:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	3	36	theme	cross-linked	406:417	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	3	37	used	used	531:534	arg2	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	2	38	theme	modification	356:367	arg1	activity					324:331	antibacterial activity	310:331	antibacterial activity	310:331	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	38	theme	modification	356:367	arg1	biodegradability					374:389	biodegradability	374:389	biodegradability	374:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	38	theme	modification	356:367	arg1	ease					348:351	ease	348:351	ease of modification	348:367	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	38	theme	modification	356:367	arg1	non-toxicity					334:345	non-toxicity	334:345	non-toxicity	334:345	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	12	39	theme	low	1885:1887	arg1	0.0044 fM					1917:1925	0.0044 fM	1917:1925	0.0044 fM	1917:1925	The low limit of quantification was 0.0044 fM.
30458193	12	39	theme	low	1885:1887	arg1	limit					1889:1893	The low limit	1881:1893	The low limit of quantification	1881:1911	The low limit of quantification was 0.0044 fM.
30458193	6	40	theme	high	1167:1170	arg1	aptamer					1186:1192	the aptamer	1182:1192	the aptamer	1182:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	6	40	theme	high	1167:1170	arg1	amount					1172:1177	a high amount	1165:1177	a high amount of the aptamer	1165:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	2	41	theme	applications	256:267	arg1	variety					245:251	a variety	243:251	a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	243:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	41	theme	applications	256:267	arg1	applications					256:267	applications	256:267	applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	256:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	42	theme	bioactive	220:228	arg1	Chitosan					186:193	Chitosan	186:193	Chitosan as a polysaccharide	186:213	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	42	theme	bioactive	220:228	arg1	polymer					230:236	a bioactive polymer	218:236	a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	218:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	3	43	theme	gold	509:512	arg1	nanoparticle					514:525	gold nanoparticle	509:525	gold nanoparticle	509:525	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	2	44	theme	functional	280:289	arg1	activity					324:331	antibacterial activity	310:331	antibacterial activity	310:331	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	44	theme	functional	280:289	arg1	ease					348:351	ease	348:351	ease of modification	348:367	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	44	theme	functional	280:289	arg1	biodegradability					374:389	biodegradability	374:389	biodegradability	374:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	44	theme	functional	280:289	arg1	properties					291:300	its functional properties	276:300	its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	276:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	2	44	theme	functional	280:289	arg1	non-toxicity					334:345	non-toxicity	334:345	non-toxicity	334:345	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	10	45	theme	aptamer	1568:1574	arg1	assay					1582:1586	the EC aptamer based assay	1561:1586	the EC aptamer based assay	1561:1586	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	11	46	theme	pulse	1685:1689	arg1	voltammetry					1691:1701	differential pulse voltammetry	1672:1701	differential pulse voltammetry	1672:1701	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	11	47	from	19.55 μM	1871:1878	arg1	range					1826:1830	a wide dynamic range	1811:1830	a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM	1811:1878	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	6	48	theme	high	1133:1136	arg1	area					1146:1149	a high surface area	1131:1149	a high surface area to immobilize a high amount of the aptamer	1131:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	6	49	used	used	1056:1059	arg2	methodology					1040:1050	Fully electrochemical methodology	1018:1050	Fully electrochemical methodology	1018:1050	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	10	50	theme	assay	1582:1586	arg1	process					1550:1556	The fabrication process	1534:1556	The fabrication process of the EC aptamer based assay	1534:1586	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	4	51	theme	thiolated	678:686	arg1	CS					727:728	CS	727:728	CS	727:728	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	51	theme	thiolated	678:686	arg1	GQDs					711:714	GQDs	711:714	GQDs	711:714	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	51	theme	thiolated	678:686	arg1	dots					705:708	thiolated graphene quantum dots	678:708	thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC	678:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	6	52	theme	electrochemical	1024:1038	arg1	methodology					1040:1050	Fully electrochemical methodology	1018:1050	Fully electrochemical methodology	1018:1050	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	6	53	theme	aptamer	1186:1192	arg1	aptamer					1186:1192	the aptamer	1182:1192	the aptamer	1182:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	6	53	theme	aptamer	1186:1192	arg1	amount					1172:1177	a high amount	1165:1177	a high amount of the aptamer	1165:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	4	54	theme	quantum	697:703	arg1	CS					727:728	CS	727:728	CS	727:728	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	54	theme	quantum	697:703	arg1	GQDs					711:714	GQDs	711:714	GQDs	711:714	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	54	theme	quantum	697:703	arg1	dots					705:708	thiolated graphene quantum dots	678:708	thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC	678:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	11	55	theme	concentration	1839:1851	arg1	range					1826:1830	a wide dynamic range	1811:1830	a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM	1811:1878	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	3	56	link	cross-linked	406:417	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	5	57	theme	gold	842:845	arg1	nanostructures					847:860	gold nanostructures	842:860	gold nanostructures with various sizes from zero-dimensional nanoparticles	842:915	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	1	58	contain	has	129:131	arg1	Chitosan					120:127	Chitosan	120:127	Chitosan	120:127	Chitosan has a number of commercial and possible biomedical uses.
30458193	1	58	contain	has	129:131	arg2	number					135:140	a number	133:140	a number of commercial and possible biomedical uses	133:183	Chitosan has a number of commercial and possible biomedical uses.
30458193	9	59	theme	membrane	1511:1518	arg1	NSs					1490:1492	the Au NSs	1483:1492	the Au NSs of the composite membrane	1483:1518	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	8	60	theme	immobilization	1312:1325	arg1	media					1327:1331	A special immobilization media	1302:1331	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA)	1302:1378	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	9	61	theme	AuS	1524:1526	arg1	bond					1528:1531	AuS bond	1524:1531	AuS bond	1524:1531	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	2	62	theme	due	269:271	arg1	applications					256:267	applications	256:267	applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability	256:389	Chitosan as a polysaccharide is a bioactive polymer with a variety of applications due to its functional properties such as antibacterial activity, non-toxicity, ease of modification, and biodegradability.
30458193	5	63	theme	zero-dimensional	886:901	arg1	nanoparticles					903:915	zero-dimensional nanoparticles	886:915	zero-dimensional nanoparticles	886:915	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	8	64	theme	Au	1347:1348	arg1	NSs/GQDs-CS/Cysteamine					1350:1371	Au NSs/GQDs-CS/Cysteamine	1347:1371	Au NSs/GQDs-CS/Cysteamine (CysA)	1347:1378	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	8	64	theme	Au	1347:1348	arg1	CysA					1374:1377	CysA	1374:1377	CysA	1374:1377	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	9	65	attach	attached	1471:1478	arg2	aptamer					1459:1465	The RAC aptamer	1451:1465	The RAC aptamer	1451:1465	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	9	65	attach	attached	1471:1478	arg3	NSs					1490:1492	the Au NSs	1483:1492	the Au NSs of the composite membrane	1483:1518	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	11	66	theme	dynamic	1818:1824	arg1	range					1826:1830	a wide dynamic range	1811:1830	a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM	1811:1878	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	7	67	theme	free	1214:1217	arg1	EC					1236:1237	EC	1236:1237	EC	1236:1237	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	7	67	theme	free	1214:1217	arg1	electrochemical					1219:1233	free electrochemical	1214:1233	a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC	1206:1285	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	11	68	dep	19.55 μM	1871:1878	arg1	to					1868:1869	to	1868:1869	to	1868:1869	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	5	69	from	nanoparticles	903:915	arg1	sizes					875:879	various sizes	867:879	various sizes from zero-dimensional nanoparticles	867:915	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	3	70	theme	biocompatible	464:476	arg1	polysaccharide					478:491	a biocompatible polysaccharide	462:491	a biocompatible polysaccharide	462:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	0	71	theme	chitosan/thiolated	13:30	arg1	polysaccharide					73:86	a biocompatible polysaccharide	57:86	a biocompatible polysaccharide towards aptamer immobilization	57:117	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	0	71	theme	chitosan/thiolated	13:30	arg1	dots					49:52	chitosan/thiolated graphene quantum dots	13:52	chitosan/thiolated graphene quantum dots	13:52	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	5	72	theme	one-step	958:965	arg1	method					1010:1015	one-step template-assistant green electrodeposition method	958:1015	one-step template-assistant green electrodeposition method	958:1015	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	0	73	theme	quantum	41:47	arg1	polysaccharide					73:86	a biocompatible polysaccharide	57:86	a biocompatible polysaccharide towards aptamer immobilization	57:117	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	0	73	theme	quantum	41:47	arg1	dots					49:52	chitosan/thiolated graphene quantum dots	13:52	chitosan/thiolated graphene quantum dots	13:52	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	5	74	theme	green	986:990	arg1	method					1010:1015	one-step template-assistant green electrodeposition method	958:1015	one-step template-assistant green electrodeposition method	958:1015	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	6	75	theme	carbon	1101:1106	arg1	surface					1108:1114	a glassy carbon surface	1092:1114	a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer	1092:1192	Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon surface which provided a high surface area to immobilize a high amount of the aptamer.
30458193	4	76	theme	specific	594:601	arg1	5'-SH-AAAAAGTGCGGGC-3					616:636	5'-SH-AAAAAGTGCGGGC-3	616:636	5'-SH-AAAAAGTGCGGGC-3	616:636	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	4	76	theme	specific	594:601	arg1	DNA-aptamer					603:613	A highly specific DNA-aptamer	585:613	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3')	585:638	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	5	77	with	nanostructures	847:860	arg1	sizes					875:879	various sizes	867:879	various sizes from zero-dimensional nanoparticles	867:915	Different shapes of gold nanostructures with various sizes from zero-dimensional nanoparticles to spherical structures were prepared by one-step template-assistant green electrodeposition method.
30458193	1	78	dep	biomedical	169:178	arg1	uses					180:183	uses	180:183	uses	180:183	Chitosan has a number of commercial and possible biomedical uses.
30458193	3	79	theme	quantum	447:453	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	0	80	theme	biocompatible	59:71	arg1	polysaccharide					73:86	a biocompatible polysaccharide	57:86	a biocompatible polysaccharide towards aptamer immobilization	57:117	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	0	80	theme	biocompatible	59:71	arg1	dots					49:52	chitosan/thiolated graphene quantum dots	13:52	chitosan/thiolated graphene quantum dots	13:52	Cross-linked chitosan/thiolated graphene quantum dots as a biocompatible polysaccharide towards aptamer immobilization.
30458193	3	81	theme	chitosan/thiolated	419:436	arg1	dot					455:457	cross-linked chitosan/thiolated graphene quantum dot	406:457	cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide	406:491	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
30458193	10	82	theme	fabrication	1538:1548	arg1	process					1550:1556	The fabrication process	1534:1556	The fabrication process of the EC aptamer based assay	1534:1586	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	8	83	theme	biosensor	1440:1448	arg1	conductivity					1404:1415	conductivity	1404:1415	conductivity	1404:1415	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	8	83	theme	biosensor	1440:1448	arg1	performance					1421:1431	performance	1421:1431	performance	1421:1431	A special immobilization media consisting of Au NSs/GQDs-CS/Cysteamine (CysA) was utilized to improve conductivity and performance of the biosensor.
30458193	12	84	theme	quantification	1898:1911	arg1	0.0044 fM					1917:1925	0.0044 fM	1917:1925	0.0044 fM	1917:1925	The low limit of quantification was 0.0044 fM.
30458193	12	84	theme	quantification	1898:1911	arg1	limit					1889:1893	The low limit	1881:1893	The low limit of quantification	1881:1911	The low limit of quantification was 0.0044 fM.
30458193	4	85	dep	dots	705:708	arg1	nanocomposite					731:743	nanocomposite	731:743	nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC	731:819	A highly specific DNA-aptamer (5'-SH-AAAAAGTGCGGGC-3'), selected to RAC was immobilized onto thiolated graphene quantum dots (GQDs)-chitosan (CS) nanocomposite modified by gold nanostructures (Au NSs) and used for quantification of RAC.
30458193	11	86	theme	peak	1646:1649	arg1	currents					1651:1658	The peak currents	1642:1658	The peak currents obtained by differential pulse voltammetry	1642:1701	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	9	87	theme	composite	1501:1509	arg1	membrane					1511:1518	the composite membrane	1497:1518	the composite membrane	1497:1518	The RAC aptamer was attached on the Au NSs of the composite membrane via AuS bond.
30458193	7	88	theme	RAC	1283:1285	arg1	detection					1270:1278	ultrasensitive detection	1255:1278	ultrasensitive detection of RAC	1255:1285	Therefore, a label free electrochemical (EC) apta-assay for ultrasensitive detection of RAC was developed.
30458193	10	89	theme	EC	1565:1566	arg1	assay					1582:1586	the EC aptamer based assay	1561:1586	the EC aptamer based assay	1561:1586	The fabrication process of the EC aptamer based assay was characterized by some electrochemical techniques.
30458193	11	90	theme	differential	1672:1683	arg1	voltammetry					1691:1701	differential pulse voltammetry	1672:1701	differential pulse voltammetry	1672:1701	The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the apta-assay responds approximately over a wide dynamic range of RAC concentration from 0.0044 fM to 19.55 μM.
30458193	3	91	theme	ractopamine	558:568	arg1	aptamer					576:582	ractopamine (RAC) aptamer	558:582	ractopamine (RAC) aptamer	558:582	In this work, cross-linked chitosan/thiolated graphene quantum dot as a biocompatible polysaccharide was modified by gold nanoparticle and used for immobilization of ractopamine (RAC) aptamer.
31791726	0	0	theme	edible	86:91	arg1	quality					93:99	the edible quality	82:99	the edible quality of rice cultivars	82:117	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	0	1	from	distribution	24:35	arg1	layers					56:61	different grain layers	40:61	different grain layers	40:61	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	2	2	theme	protein	541:547	arg1	acids					569:573	amino acids	563:573	amino acids	563:573	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	2	theme	protein	541:547	arg1	minerals					596:603	minerals	596:603	minerals	596:603	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	2	theme	protein	541:547	arg1	content					526:532	apparent amylose content	509:532	apparent amylose content (AAC)	509:538	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	2	theme	protein	541:547	arg1	content					549:555	protein content	541:555	protein content (PC)	541:560	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	2	theme	protein	541:547	arg1	PC					558:559	PC	558:559	PC	558:559	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	2	theme	protein	541:547	arg1	sugars					584:589	soluble sugars	576:589	soluble sugars	576:589	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	1	3	from	composition	187:197	arg1	association					135:145	the association	131:145	the association of differences in endosperm biochemical composition with nutrient and edible quality	131:230	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	2	4	theme	Northeast	347:355	arg1	CTC					375:377	CTC	375:377	CTC	375:377	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	4	theme	Northeast	347:355	arg1	good-taste					363:372	Northeast China good-taste	347:372	Northeast China good-taste (CTC)	347:378	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	6	5	from	L2	910:911	arg1	levels					886:891	High levels	881:891	High levels of amino acid in L2 and L3	881:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	1	6	theme	endosperm	165:173	arg1	composition					187:197	endosperm biochemical composition	165:197	endosperm biochemical composition	165:197	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	6	7	from	levels	886:891	arg1	L2					910:911	L2	910:911	L2	910:911	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	6	7	from	levels	886:891	arg1	L3					917:918	L3	917:918	L3	917:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	4	8	theme	highest	768:774	arg1	value					782:786	the highest taste value	764:786	the highest taste value	764:786	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	6	9	from	L3	917:918	arg1	levels					886:891	High levels	881:891	High levels of amino acid in L2 and L3	881:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	3	10	with	association	610:620	arg1	taste					681:685	taste	681:685	taste	681:685	The association of these compositions and Rapid-Visco analysis values with taste was also examined.
31791726	5	11	theme	AAC	825:827	arg1	sugar					841:845	AAC and soluble sugar	825:845	AAC and soluble sugar in L3	825:851	Taste was significantly affected by AAC and soluble sugar in L3, and by PC in the L2 layer.
31791726	3	12	theme	compositions	631:642	arg1	association					610:620	The association	606:620	The association of these compositions and Rapid-Visco analysis values with taste	606:685	The association of these compositions and Rapid-Visco analysis values with taste was also examined.
31791726	2	13	theme	amino	563:567	arg1	acids					569:573	amino acids	563:573	amino acids	563:573	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	13	theme	amino	563:567	arg1	content					549:555	protein content	541:555	protein content (PC)	541:560	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	14	theme	very-low-amylose-content	288:311	arg1	cultivars					418:426	very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars	288:426	very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars	288:426	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	0	15	theme	cultivars	109:117	arg1	quality					93:99	the edible quality	82:99	the edible quality of rice cultivars	82:117	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	1	16	from	differences	150:160	arg1	composition					187:197	endosperm biochemical composition	165:197	endosperm biochemical composition	165:197	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	6	17	theme	High	881:884	arg1	levels					886:891	High levels	881:891	High levels of amino acid in L2 and L3	881:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	1	18	theme	biochemical	175:185	arg1	composition					187:197	endosperm biochemical composition	165:197	endosperm biochemical composition	165:197	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	0	19	theme	rice	104:107	arg1	cultivars					109:117	rice cultivars	104:117	rice cultivars	104:117	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	1	20	with	association	135:145	arg1	nutrient					204:211	nutrient	204:211	nutrient	204:211	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	1	20	with	association	135:145	arg1	quality					224:230	edible quality	217:230	edible quality	217:230	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	0	21	theme	composition	12:22	arg1	distribution					24:35	Biochemical composition distribution	0:35	Biochemical composition distribution in different grain layers	0:61	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	1	22	from	association	135:145	arg1	composition					187:197	endosperm biochemical composition	165:197	endosperm biochemical composition	165:197	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	2	23	theme	Japanese	320:327	arg1	JTC					341:343	JTC	341:343	JTC	341:343	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	23	theme	Japanese	320:327	arg1	good-taste					329:338	Japanese good-taste	320:338	Japanese good-taste (JTC)	320:344	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	23	theme	Japanese	320:327	arg1	CHC					413:415	CHC	413:415	CHC	413:415	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	5	24	from	PC	861:862	arg1	layer					874:878	the L2 layer	867:878	the L2 layer	867:878	Taste was significantly affected by AAC and soluble sugar in L3, and by PC in the L2 layer.
31791726	0	25	theme	Biochemical	0:10	arg1	distribution					24:35	Biochemical composition distribution	0:35	Biochemical composition distribution in different grain layers	0:61	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	3	26	theme	values	669:674	arg1	association					610:620	The association	606:620	The association of these compositions and Rapid-Visco analysis values with taste	606:685	The association of these compositions and Rapid-Visco analysis values with taste was also examined.
31791726	4	27	theme	mineral	743:749	arg1	content					751:757	mineral content	743:757	mineral content	743:757	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	6	28	from	acid	902:905	arg1	L2					910:911	L2	910:911	L2	910:911	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	6	28	from	acid	902:905	arg1	L3					917:918	L3	917:918	L3	917:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	2	29	theme	soluble	576:582	arg1	sugars					584:589	soluble sugars	576:589	soluble sugars	576:589	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	29	theme	soluble	576:582	arg1	content					549:555	protein content	541:555	protein content (PC)	541:560	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	5	30	theme	soluble	833:839	arg1	sugar					841:845	AAC and soluble sugar	825:845	AAC and soluble sugar in L3	825:851	Taste was significantly affected by AAC and soluble sugar in L3, and by PC in the L2 layer.
31791726	6	31	from	P	951:951	arg1	L1					964:965	L1	964:965	L1	964:965	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	2	32	theme	high-yield	401:410	arg1	China					395:399	Northeast China high-yield	385:410	Northeast China high-yield	385:410	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	3	33	theme	Rapid-Visco	648:658	arg1	values					669:674	Rapid-Visco analysis values	648:674	Rapid-Visco analysis values	648:674	The association of these compositions and Rapid-Visco analysis values with taste was also examined.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	CHC					413:415	CHC	413:415	CHC	413:415	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	good-taste					363:372	Northeast China good-taste	347:372	Northeast China good-taste (CTC)	347:378	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	good-taste					329:338	Japanese good-taste	320:338	Japanese good-taste (JTC)	320:344	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	China					395:399	Northeast China high-yield	385:410	Northeast China high-yield	385:410	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	JTC					341:343	JTC	341:343	JTC	341:343	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	CTC					375:377	CTC	375:377	CTC	375:377	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	34	dep	very-low-amylose-content	288:311	arg1	LAC					314:316	LAC	314:316	LAC	314:316	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	6	35	from	Ca	958:959	arg1	L1					964:965	L1	964:965	L1	964:965	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	1	36	theme	edible	217:222	arg1	quality					224:230	edible quality	217:230	edible quality	217:230	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	0	37	theme	grain	50:54	arg1	layers					56:61	different grain layers	40:61	different grain layers	40:61	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	3	38	theme	analysis	660:667	arg1	values					669:674	Rapid-Visco analysis values	648:674	Rapid-Visco analysis values	648:674	The association of these compositions and Rapid-Visco analysis values with taste was also examined.
31791726	7	39	theme	amino	1058:1062	arg1	acids					1064:1068	amino acids	1058:1068	amino acids	1058:1068	On the whole, starch factors had the highest contribution to taste, followed by minerals, amino acids, and PC.
31791726	5	40	theme	L2	871:872	arg1	layer					874:878	the L2 layer	867:878	the L2 layer	867:878	Taste was significantly affected by AAC and soluble sugar in L3, and by PC in the L2 layer.
31791726	2	41	theme	Northeast	385:393	arg1	China					395:399	Northeast China high-yield	385:410	Northeast China high-yield	385:410	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	0	42	theme	different	40:48	arg1	layers					56:61	different grain layers	40:61	different grain layers	40:61	Biochemical composition distribution in different grain layers is associated with the edible quality of rice cultivars.
31791726	6	43	theme	amino	896:900	arg1	acid					902:905	amino acid	896:905	amino acid in L2 and L3	896:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	4	44	theme	amino	728:732	arg1	acid					734:737	high amino acid	723:737	high amino acid	723:737	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	4	45	theme	taste	776:780	arg1	value					782:786	the highest taste value	764:786	the highest taste value	764:786	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	4	46	theme	high	723:726	arg1	acid					734:737	high amino acid	723:737	high amino acid	723:737	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	2	47	theme	amylose	518:524	arg1	AAC					535:537	AAC	535:537	AAC	535:537	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	47	theme	amylose	518:524	arg1	content					526:532	apparent amylose content	509:532	apparent amylose content (AAC)	509:538	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	47	theme	amylose	518:524	arg1	content					549:555	protein content	541:555	protein content (PC)	541:560	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	6	48	dep	did	943:945	arg1	as					940:941	as	940:941	as	940:941	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	6	49	from	Mg	947:948	arg1	L1					964:965	L1	964:965	L1	964:965	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	5	50	from	sugar	841:845	arg1	L3					850:851	L3	850:851	L3	850:851	Taste was significantly affected by AAC and soluble sugar in L3, and by PC in the L2 layer.
31791726	4	51	contain	had	710:712	arg2	acid					734:737	high amino acid	723:737	high amino acid	723:737	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	4	51	contain	had	710:712	arg1	LAC					706:708	LAC	706:708	LAC	706:708	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	4	51	contain	had	710:712	arg2	value					782:786	the highest taste value	764:786	the highest taste value	764:786	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	4	51	contain	had	710:712	arg2	AAC					718:720	low AAC	714:720	low AAC	714:720	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	2	52	theme	apparent	509:516	arg1	AAC					535:537	AAC	535:537	AAC	535:537	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	52	theme	apparent	509:516	arg1	content					526:532	apparent amylose content	509:532	apparent amylose content (AAC)	509:538	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	52	theme	apparent	509:516	arg1	content					549:555	protein content	541:555	protein content (PC)	541:560	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	7	53	contain	had	997:999	arg2	contribution					1013:1024	the highest contribution	1001:1024	the highest contribution to taste	1001:1033	On the whole, starch factors had the highest contribution to taste, followed by minerals, amino acids, and PC.
31791726	7	53	contain	had	997:999	arg1	factors					989:995	starch factors	982:995	starch factors	982:995	On the whole, starch factors had the highest contribution to taste, followed by minerals, amino acids, and PC.
31791726	2	54	theme	China	357:361	arg1	CTC					375:377	CTC	375:377	CTC	375:377	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	2	54	theme	China	357:361	arg1	good-taste					363:372	Northeast China good-taste	347:372	Northeast China good-taste (CTC)	347:378	In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals.
31791726	1	55	theme	rice	242:245	arg1	breeding					247:254	rice breeding	242:254	rice breeding	242:254	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	4	56	theme	low	714:716	arg1	AAC					718:720	low AAC	714:720	low AAC	714:720	LAC had low AAC, high amino acid and mineral content, and the highest taste value.
31791726	6	57	theme	acid	902:905	arg1	levels					886:891	High levels	881:891	High levels of amino acid in L2 and L3	881:918	High levels of amino acid in L2 and L3 improved the taste, as did Mg, P, and Ca in L1.
31791726	7	58	theme	highest	1005:1011	arg1	contribution					1013:1024	the highest contribution	1001:1024	the highest contribution to taste	1001:1033	On the whole, starch factors had the highest contribution to taste, followed by minerals, amino acids, and PC.
31791726	1	59	theme	differences	150:160	arg1	association					135:145	the association	131:145	the association of differences in endosperm biochemical composition with nutrient and edible quality	131:230	Clarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation.
31791726	7	60	theme	starch	982:987	arg1	factors					989:995	starch factors	982:995	starch factors	982:995	On the whole, starch factors had the highest contribution to taste, followed by minerals, amino acids, and PC.
31678100	5	0	theme	total	759:763	arg1	capacity					777:784	The total antioxidant capacity	755:784	The total antioxidant capacity of LWSP at 12 mg/ml	755:804	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	5	0	theme	total	759:763	arg1	equivalent					810:819	equivalent	810:819	equivalent	810:819	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	6	1	theme	byhydroxyl	916:925	arg1	radical					927:933	byhydroxyl radical	916:933	byhydroxyl radical	916:933	LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical.
31678100	6	2	theme	protection	871:880	arg1	effect					882:887	a high protection effect	864:887	a high protection effect	864:887	LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical.
31678100	1	3	theme	present	153:159	arg1	study					161:165	The present study	149:165	The present study	149:165	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	6	4	theme	DNA	897:899	arg1	damage					901:906	DNA damage	897:906	DNA damage induced byhydroxyl radical	897:933	LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical.
31678100	1	5	theme	novel	249:253	arg1	LWSP					285:288	LWSP	285:288	LWSP	285:288	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	5	theme	novel	249:253	arg1	polysaccharide					269:282	a novel water soluble polysaccharide	247:282	a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds	247:333	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	6	from	burn	411:414	arg1	model					425:429	a rat model	419:429	a rat model	419:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	7	theme	rat	421:423	arg1	model					425:429	a rat model	419:429	a rat model	419:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	12	8	theme	67 ± 92.28 mg/g	1446:1460	arg1	tissue					1462:1467	67 ± 92.28 mg/g tissue)	1446:1468	67 ± 92.28 mg/g tissue)	1446:1468	67 ± 92.28 mg/g tissue) than the other groups.
31678100	0	9	theme	usitatissimum	105:117	arg1	seeds					122:126	Linum usitatissimum L. seeds	99:126	Linum usitatissimum L. seeds on laser burn wound	99:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	7	10	theme	human	979:983	arg1	erythrocytes					985:996	human erythrocytes	979:996	human erythrocytes	979:996	No hemolytic activity was observed towards human erythrocytes.
31678100	10	11	from	application	1189:1199	arg1	site					1227:1230	the burn wound site	1212:1230	the burn wound site in rat model	1212:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	1	12	theme	soluble	261:267	arg1	LWSP					285:288	LWSP	285:288	LWSP	285:288	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	12	theme	soluble	261:267	arg1	polysaccharide					269:282	a novel water soluble polysaccharide	247:282	a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds	247:333	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	0	13	theme	Linum	99:103	arg1	seeds					122:126	Linum usitatissimum L. seeds	99:126	Linum usitatissimum L. seeds on laser burn wound	99:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	1	14	theme	in	353:354	arg1	healing					367:373	the in vivo wound healing	349:373	the in vivo wound healing performance on CO2 laser fractional burn in a rat model	349:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	10	15	theme	contraction	1292:1302	arg1	percentage					1273:1282	the percentage	1269:1282	the percentage of burn contraction (98.6%) after 8 days of injury	1269:1333	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	11	16	theme	LWSP-treated	1382:1393	arg1	group					1395:1399	the LWSP-treated group	1378:1399	the LWSP-treated group	1378:1399	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31678100	3	17	theme	different	526:534	arg1	tests					536:540	Three different tests	520:540	Three different tests	520:540	Three different tests were used to evaluate the antioxidant activity of this carbohydrate.
31678100	9	18	theme	high	1089:1092	arg1	WHC					1101:1103	WHC	1101:1103	WHC	1101:1103	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	9	18	theme	high	1089:1092	arg1	water					1094:1098	high water	1089:1098	high water (WHC)	1089:1104	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	1	19	theme	polysaccharide	269:282	arg1	purification					184:195	the purification	180:195	the purification	180:195	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	19	theme	polysaccharide	269:282	arg1	activities					233:242	in vitro antioxidant activities	212:242	in vitro antioxidant activities	212:242	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	19	theme	polysaccharide	269:282	arg1	structure					198:206	structure	198:206	structure	198:206	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	20	from	performance	375:385	arg1	burn					411:414	CO2 laser fractional burn	390:414	CO2 laser fractional burn in a rat model	390:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	13	21	theme	wound	1609:1613	arg1	healing					1615:1621	burn wound healing	1604:1621	burn wound healing	1604:1621	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	9	22	theme	emulsion	1065:1072	arg1	properties					1074:1083	good emulsion properties	1060:1083	good emulsion properties	1060:1083	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	9	23	theme	water	1094:1098	arg1	capacities					1128:1137	high water (WHC) and oil holding (OHC) capacities	1089:1137	high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively)	1089:1169	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	0	24	theme	L.	119:120	arg1	seeds					122:126	Linum usitatissimum L. seeds	99:126	Linum usitatissimum L. seeds on laser burn wound	99:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	1	25	theme	wound	361:365	arg1	healing					367:373	the in vivo wound healing	349:373	the in vivo wound healing performance on CO2 laser fractional burn in a rat model	349:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	12	26	dep	tissue	1462:1467	arg1	groups					1485:1490	the other groups	1475:1490	the other groups	1475:1490	67 ± 92.28 mg/g tissue) than the other groups.
31678100	7	27	theme	hemolytic	939:947	arg1	activity					949:956	No hemolytic activity	936:956	No hemolytic activity	936:956	No hemolytic activity was observed towards human erythrocytes.
31678100	13	28	theme	promising	1576:1584	arg1	opportunities					1586:1598	promising opportunities	1576:1598	promising opportunities for burn wound healing in modern medicine	1576:1640	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	1	29	dep	in	353:354	arg1	vivo					356:359	vivo	356:359	vivo	356:359	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	1	30	theme	healing	367:373	arg1	performance					375:385	the in vivo wound healing performance	349:385	the in vivo wound healing performance on CO2 laser fractional burn in a rat model	349:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	4	31	theme	acid	693:696	arg1	radical					705:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	13	32	theme	modern	1626:1631	arg1	medicine					1633:1640	modern medicine	1626:1640	modern medicine	1626:1640	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	0	33	from	seeds	122:126	arg1	wound					142:146	laser burn wound	131:146	laser burn wound	131:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	4	34	theme	3-ethylbenzothiazoline-6-sulphonic	658:691	arg1	radical					705:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	10	35	theme	rat	1235:1237	arg1	model					1239:1243	rat model	1235:1243	rat model	1235:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	0	36	theme	laser	131:135	arg1	wound					142:146	laser burn wound	131:146	laser burn wound	131:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	8	37	theme	functional	1018:1027	arg1	properties					1029:1038	functional properties	1018:1038	functional properties	1018:1038	LWSP was tested in functional properties.
31678100	11	38	theme	hydroxyproline	1425:1438	arg1	content					1414:1420	a higher content	1405:1420	a higher content of hydroxyproline (846	1405:1443	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31678100	4	39	theme	scavenging	615:624	arg1	activity					626:633	The scavenging activity	611:633	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml	611:741	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	4	39	theme	scavenging	615:624	arg1	%					752:752	99.77%	747:752	99.77%	747:752	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	4	40	theme	5 mg/ml	735:741	arg1	concentration					718:730	a concentration	716:730	a concentration of 5 mg/ml	716:741	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	6	41	theme	high	866:869	arg1	effect					882:887	a high protection effect	864:887	a high protection effect	864:887	LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical.
31678100	9	42	theme	oil	1110:1112	arg1	capacities					1128:1137	high water (WHC) and oil holding (OHC) capacities	1089:1137	high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively)	1089:1169	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	5	43	theme	acsorbic	834:841	arg1	acid					843:846	166.61 µg acsorbic acid	824:846	166.61 µg acsorbic acid	824:846	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	9	44	dep	capacities	1128:1137	arg1	%					1154:1154	11.23 and 1.05%	1140:1154	11.23 and 1.05%	1140:1154	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	10	45	theme	wound	1221:1225	arg1	site					1227:1230	the burn wound site	1212:1230	the burn wound site in rat model	1212:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	3	46	theme	antioxidant	568:578	arg1	activity					580:587	the antioxidant activity	564:587	the antioxidant activity of this carbohydrate	564:608	Three different tests were used to evaluate the antioxidant activity of this carbohydrate.
31678100	9	47	theme	holding	1114:1120	arg1	oil					1110:1112	oil holding	1110:1120	oil holding (OHC)	1110:1126	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	9	47	theme	holding	1114:1120	arg1	OHC					1123:1125	OHC	1123:1125	OHC	1123:1125	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	1	48	theme	in	212:213	arg1	activities					233:242	in vitro antioxidant activities	212:242	in vitro antioxidant activities	212:242	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	0	49	theme	biological	30:39	arg1	activities					41:50	biological activities	30:50	biological activities	30:50	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	0	50	theme	burn	137:140	arg1	wound					142:146	laser burn wound	131:146	laser burn wound	131:146	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	1	51	theme	CO2	390:392	arg1	burn					411:414	CO2 laser fractional burn	390:414	CO2 laser fractional burn in a rat model	390:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	10	52	from	site	1227:1230	arg1	model					1239:1243	rat model	1235:1243	rat model	1235:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	3	53	used	used	547:550	arg2	tests					536:540	Three different tests	520:540	Three different tests	520:540	Three different tests were used to evaluate the antioxidant activity of this carbohydrate.
31678100	5	54	theme	LWSP	789:792	arg1	capacity					777:784	The total antioxidant capacity	755:784	The total antioxidant capacity of LWSP at 12 mg/ml	755:804	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	5	54	theme	LWSP	789:792	arg1	equivalent					810:819	equivalent	810:819	equivalent	810:819	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	1	55	theme	Linum	306:310	arg1	seeds					329:333	Linum usitatissimum L. seeds	306:333	Linum usitatissimum L. seeds	306:333	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	13	56	theme	novel	1556:1560	arg1	LWSP					1562:1565	this novel LWSP	1551:1565	this novel LWSP	1551:1565	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	1	57	theme	laser	394:398	arg1	burn					411:414	CO2 laser fractional burn	390:414	CO2 laser fractional burn in a rat model	390:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	5	58	from	12 mg/ml	797:804	arg1	capacity					777:784	The total antioxidant capacity	755:784	The total antioxidant capacity of LWSP at 12 mg/ml	755:804	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	5	58	from	12 mg/ml	797:804	arg1	equivalent					810:819	equivalent	810:819	equivalent	810:819	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	12	59	theme	other	1479:1483	arg1	groups					1485:1490	the other groups	1475:1490	the other groups	1475:1490	67 ± 92.28 mg/g tissue) than the other groups.
31678100	13	60	theme	burn	1604:1607	arg1	healing					1615:1621	burn wound healing	1604:1621	burn wound healing	1604:1621	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	1	61	theme	usitatissimum	312:324	arg1	seeds					329:333	Linum usitatissimum L. seeds	306:333	Linum usitatissimum L. seeds	306:333	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	13	62	theme	LWSP	1562:1565	arg1	application					1536:1546	the application	1532:1546	the application of this novel LWSP	1532:1565	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	1	63	theme	fractional	400:409	arg1	burn					411:414	CO2 laser fractional burn	390:414	CO2 laser fractional burn in a rat model	390:429	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	10	64	theme	burn	1287:1290	arg1	contraction					1292:1302	burn contraction	1287:1302	burn contraction (98.6%) after 8 days of injury	1287:1333	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	10	64	theme	burn	1287:1290	arg1	%					1309:1309	98.6%	1305:1309	98.6%	1305:1309	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	10	65	theme	LWSP	1204:1207	arg1	application					1189:1199	the application	1185:1199	the application of LWSP on the burn wound site in rat model	1185:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	1	66	theme	L.	326:327	arg1	seeds					329:333	Linum usitatissimum L. seeds	306:333	Linum usitatissimum L. seeds	306:333	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	3	67	theme	carbohydrate	597:608	arg1	activity					580:587	the antioxidant activity	564:587	the antioxidant activity of this carbohydrate	564:608	Three different tests were used to evaluate the antioxidant activity of this carbohydrate.
31678100	1	68	dep	in	212:213	arg1	vitro					215:219	vitro	215:219	vitro	215:219	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	10	69	theme	injury	1328:1333	arg1	8 days					1318:1323	8 days	1318:1323	8 days of injury	1318:1333	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	4	70	from	concentration	718:730	arg1	activity					626:633	The scavenging activity	611:633	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml	611:741	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	4	70	from	concentration	718:730	arg1	%					752:752	99.77%	747:752	99.77%	747:752	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	1	71	theme	antioxidant	221:231	arg1	activities					233:242	in vitro antioxidant activities	212:242	in vitro antioxidant activities	212:242	The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model.
31678100	5	72	theme	antioxidant	765:775	arg1	capacity					777:784	The total antioxidant capacity	755:784	The total antioxidant capacity of LWSP at 12 mg/ml	755:804	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	5	72	theme	antioxidant	765:775	arg1	equivalent					810:819	equivalent	810:819	equivalent	810:819	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	11	73	theme	higher	1407:1412	arg1	content					1414:1420	a higher content	1405:1420	a higher content of hydroxyproline (846	1405:1443	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31678100	13	74	from	opportunities	1586:1598	arg1	medicine					1633:1640	modern medicine	1626:1640	modern medicine	1626:1640	Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.
31678100	0	75	theme	heteropolysaccharide	63:82	arg1	composition					14:24	composition	14:24	composition	14:24	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	0	75	theme	heteropolysaccharide	63:82	arg1	activities					41:50	biological activities	30:50	biological activities	30:50	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	0	75	theme	heteropolysaccharide	63:82	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	10	76	theme	burn	1216:1219	arg1	site					1227:1230	the burn wound site	1212:1230	the burn wound site in rat model	1212:1243	In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury.
31678100	5	77	theme	166.61 µg	824:832	arg1	acid					843:846	166.61 µg acsorbic acid	824:846	166.61 µg acsorbic acid	824:846	The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid.
31678100	4	78	theme	ABTS	699:702	arg1	radical					705:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	4	79	theme	2,2'-azino-bis	643:656	arg1	radical					705:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical	643:711	The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%.
31678100	0	80	theme	novel	57:61	arg1	heteropolysaccharide					63:82	a novel heteropolysaccharide	55:82	a novel heteropolysaccharide	55:82	Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.
31678100	11	81	contain	had	1401:1403	arg2	content					1414:1420	a higher content	1405:1420	a higher content of hydroxyproline (846	1405:1443	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31678100	11	81	contain	had	1401:1403	arg1	group					1395:1399	the LWSP-treated group	1378:1399	the LWSP-treated group	1378:1399	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31678100	9	82	theme	good	1060:1063	arg1	properties					1074:1083	good emulsion properties	1060:1083	good emulsion properties	1060:1083	The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively).
31678100	11	83	theme	histological	1353:1364	arg1	assessment					1366:1375	the histological assessment	1349:1375	the histological assessment	1349:1375	According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846.
31408160	2	0	theme	type	475:478	arg1	differences					480:490	distinct cell type differences	461:490	distinct cell type differences in cell wall composition in the exocarp	461:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	1	1	theme	turgor-driven	291:303	arg1	expansion					305:313	the turgor-driven expansion	287:313	the turgor-driven expansion of the underlying thin-walled cortical cells during growth	287:372	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	1	2	theme	Malus×domestica	141:155	arg1	fruit					158:162	apple (Malus×domestica) fruit	134:162	apple (Malus×domestica) fruit	134:162	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	2	3	theme	cell	470:473	arg1	differences					480:490	distinct cell type differences	461:490	distinct cell type differences in cell wall composition in the exocarp	461:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	3	4	from	present	740:746	arg1	walls					770:774	the epidermal cell walls	751:774	the epidermal cell walls in expanding fruit	751:793	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	6	5	theme	RNA-seq	1083:1089	arg1	data					1091:1094	The RNA-seq data	1079:1094	The RNA-seq data	1079:1094	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	8	6	theme	pectin	1638:1643	arg1	remodelling					1645:1655	cell wall pectin remodelling	1628:1655	cell wall pectin remodelling	1628:1655	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	4	7	theme	cell	910:913	arg1	types					915:919	cell types	910:919	cell types	910:919	Branched (1→5)-α-l-arabinan was uniformly distributed between cell types.
31408160	3	8	theme	linear	668:673	arg1	-α-l-arabinan					680:692	linear (1→5)-α-l-arabinan	668:692	linear (1→5)-α-l-arabinan (associated with flexibility)	668:722	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	8	9	from	layers	1522:1527	arg1	fruit					1538:1542	apple fruit	1532:1542	apple fruit during development and ripening	1532:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	5	10	theme	Laser	922:926	arg1	microdissection					936:950	Laser capture microdissection	922:950	Laser capture microdissection	922:950	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	8	11	theme	wall	1633:1636	arg1	remodelling					1645:1655	cell wall pectin remodelling	1628:1655	cell wall pectin remodelling	1628:1655	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	7	12	theme	α-arabinofuranosidases	1398:1419	arg1	AF1					1421:1423	α-arabinofuranosidases AF1	1398:1423	α-arabinofuranosidases AF1	1398:1423	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	5	13	theme	capture	928:934	arg1	microdissection					936:950	Laser capture microdissection	922:950	Laser capture microdissection	922:950	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	8	14	theme	apple	1532:1536	arg1	fruit					1538:1542	apple fruit	1532:1542	apple fruit during development and ripening	1532:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	0	15	theme	apple	106:110	arg1	development					118:128	apple fruit development	106:128	apple fruit development	106:128	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	1	16	theme	underlying	322:331	arg1	cells					354:358	the underlying thin-walled cortical cells	318:358	the underlying thin-walled cortical cells	318:358	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	8	17	theme	remodelling	1645:1655	arg1	control					1617:1623	the control	1613:1623	the control of cell wall pectin remodelling	1613:1655	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	2	18	theme	cell	495:498	arg1	composition					505:515	cell wall composition	495:515	cell wall composition in the exocarp	495:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	1	19	theme	thin-walled	333:343	arg1	cells					354:358	the underlying thin-walled cortical cells	318:358	the underlying thin-walled cortical cells	318:358	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	7	20	theme	arabinans	1385:1393	arg1	β-galactosidases					1305:1320	possibly five β-galactosidases	1291:1320	possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103)	1291:1364	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	debranching					1370:1380	debranching	1370:1380	debranching of arabinans by α-arabinofuranosidases AF1 and AF2	1370:1431	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	BGAL2					1323:1327	BGAL2	1323:1327	BGAL2	1323:1327	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	BGAL11					1345:1350	BGAL11	1345:1350	BGAL11	1345:1350	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	BGAL7					1330:1334	BGAL7	1330:1334	BGAL7	1330:1334	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	BGAL103					1357:1363	BGAL103	1357:1363	BGAL103	1357:1363	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	20	theme	arabinans	1385:1393	arg1	BGAL10					1337:1342	BGAL10	1337:1342	BGAL10	1337:1342	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	2	21	theme	wall	500:503	arg1	composition					505:515	cell wall composition	495:515	cell wall composition in the exocarp	495:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	0	22	theme	type-specific	5:17	arg1	expression					24:33	Cell type-specific gene expression	0:33	Cell type-specific gene expression	0:33	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	1	23	theme	cortical	345:352	arg1	cells					354:358	the underlying thin-walled cortical cells	318:358	the underlying thin-walled cortical cells	318:358	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	7	24	theme	side	1276:1279	arg1	chains					1281:1286	galactan side chains	1267:1286	galactan side chains	1267:1286	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	25	theme	epidermal	1215:1223	arg1	cells					1225:1229	epidermal cells	1215:1229	epidermal cells	1215:1229	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	6	26	theme	composition	1135:1145	arg1	control					1114:1120	the control	1110:1120	the control of cell wall composition	1110:1145	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	4	27	theme	Branched	848:855	arg1	1→5					858:860	1→5	858:860	1→5	858:860	Branched (1→5)-α-l-arabinan was uniformly distributed between cell types.
31408160	4	27	theme	Branched	848:855	arg1	-α-l-arabinan					862:874	Branched (1→5)-α-l-arabinan	848:874	Branched (1→5)-α-l-arabinan	848:874	Branched (1→5)-α-l-arabinan was uniformly distributed between cell types.
31408160	3	28	located	present	740:746	arg1	walls					770:774	the epidermal cell walls	751:774	the epidermal cell walls in expanding fruit	751:793	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	3	28	located	present	740:746	arg2	-α-l-arabinan					680:692	linear (1→5)-α-l-arabinan	668:692	linear (1→5)-α-l-arabinan (associated with flexibility)	668:722	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	2	29	theme	exclusion	417:425	arg1	chromatography					445:458	in situ immunolocalization and size exclusion epitope detection chromatography	381:458	chromatography	445:458	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	8	30	theme	layers	1522:1527	arg1	flexibility					1475:1485	flexibility	1475:1485	flexibility	1475:1485	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	8	30	theme	layers	1522:1527	arg1	rigidity					1491:1498	rigidity	1491:1498	rigidity	1491:1498	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	8	31	from	fruit	1538:1542	arg1	flexibility					1475:1485	flexibility	1475:1485	flexibility	1475:1485	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	8	31	from	fruit	1538:1542	arg1	rigidity					1491:1498	rigidity	1491:1498	rigidity	1491:1498	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	6	32	theme	wall	1130:1133	arg1	composition					1135:1145	cell wall composition	1125:1145	cell wall composition	1125:1145	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	1	33	theme	different	169:177	arg1	layers					179:184	the different layers	165:184	the different layers of the exocarp (cuticle, epidermis, and hypodermis)	165:236	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	2	34	theme	size	412:415	arg1	chromatography					445:458	in situ immunolocalization and size exclusion epitope detection chromatography	381:458	chromatography	445:458	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	8	35	theme	cell	1517:1520	arg1	layers					1522:1527	the different cell layers	1503:1527	the different cell layers in apple fruit during development and ripening	1503:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	6	36	theme	cell	1125:1128	arg1	composition					1135:1145	cell wall composition	1125:1145	cell wall composition	1125:1145	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	3	37	theme	cell	588:591	arg1	walls					593:597	Epidermal cell walls	578:597	Epidermal cell walls	578:597	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	3	38	dep	lacked	599:604	arg1	whereas					660:666	whereas	660:666	whereas	660:666	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	0	39	theme	gene	19:22	arg1	expression					24:33	Cell type-specific gene expression	0:33	Cell type-specific gene expression	0:33	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	6	40	theme	cell-specific	1167:1179	arg1	expression					1186:1195	cell-specific gene expression	1167:1195	cell-specific gene expression of hydrolases	1167:1209	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	0	41	theme	fruit	112:116	arg1	development					118:128	apple fruit development	106:128	apple fruit development	106:128	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	1	42	theme	cells	354:358	arg1	expansion					305:313	the turgor-driven expansion	287:313	the turgor-driven expansion of the underlying thin-walled cortical cells during growth	287:372	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	8	43	theme	different	1507:1515	arg1	layers					1522:1527	the different cell layers	1503:1527	the different cell layers in apple fruit during development and ripening	1503:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	8	44	from	rigidity	1491:1498	arg1	fruit					1538:1542	apple fruit	1532:1542	apple fruit during development and ripening	1532:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	2	45	theme	distinct	461:468	arg1	differences					480:490	distinct cell type differences	461:490	distinct cell type differences in cell wall composition in the exocarp	461:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	1	46	theme	fruit	259:263	arg1	integrity					265:273	fruit integrity	259:273	fruit integrity	259:273	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	3	47	attach	present	740:746	arg1	walls					770:774	the epidermal cell walls	751:774	the epidermal cell walls in expanding fruit	751:793	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	3	47	attach	present	740:746	arg2	-α-l-arabinan					680:692	linear (1→5)-α-l-arabinan	668:692	linear (1→5)-α-l-arabinan (associated with flexibility)	668:722	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	5	48	theme	type-specific	1046:1058	arg1	modification					1065:1076	cell type-specific wall modification	1041:1076	cell type-specific wall modification	1041:1076	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	2	49	dep	in	381:382	arg1	situ					384:387	situ	384:387	situ	384:387	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	7	50	theme	chains	1281:1286	arg1	degradation					1252:1262	the degradation	1248:1262	the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2	1248:1431	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	2	51	theme	in	381:382	arg1	immunolocalization					389:406	in situ immunolocalization and size exclusion epitope detection chromatography	381:458	immunolocalization	389:406	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	5	52	theme	wall	1060:1063	arg1	modification					1065:1076	cell type-specific wall modification	1041:1076	cell type-specific wall modification	1041:1076	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	β-galactosidases					1305:1320	possibly five β-galactosidases	1291:1320	possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103)	1291:1364	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	BGAL2					1323:1327	BGAL2	1323:1327	BGAL2	1323:1327	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	BGAL11					1345:1350	BGAL11	1345:1350	BGAL11	1345:1350	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	BGAL7					1330:1334	BGAL7	1330:1334	BGAL7	1330:1334	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	BGAL103					1357:1363	BGAL103	1357:1363	BGAL103	1357:1363	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	7	53	dep	β-galactosidases	1305:1320	arg1	BGAL10					1337:1342	BGAL10	1337:1342	BGAL10	1337:1342	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	1	54	theme	exocarp	193:199	arg1	layers					179:184	the different layers	165:184	the different layers of the exocarp (cuticle, epidermis, and hypodermis)	165:236	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	0	55	theme	cell	60:63	arg1	pectin					70:75	cell wall pectin	60:75	cell wall pectin	60:75	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	2	56	from	differences	480:490	arg1	composition					505:515	cell wall composition	495:515	cell wall composition in the exocarp	495:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	3	57	from	walls	770:774	arg1	present					740:746	present	740:746	present	740:746	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	2	58	theme	detection	435:443	arg1	chromatography					445:458	in situ immunolocalization and size exclusion epitope detection chromatography	381:458	chromatography	445:458	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	2	59	theme	fruit	559:563	arg1	development					565:575	apple fruit development	553:575	apple fruit development	553:575	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	8	60	from	flexibility	1475:1485	arg1	fruit					1538:1542	apple fruit	1532:1542	apple fruit during development and ripening	1532:1574	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	3	61	theme	Epidermal	578:586	arg1	walls					593:597	Epidermal cell walls	578:597	Epidermal cell walls	578:597	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	7	62	theme	galactan	1267:1274	arg1	chains					1281:1286	galactan side chains	1267:1286	galactan side chains	1267:1286	In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2.
31408160	6	63	theme	gene	1181:1184	arg1	expression					1186:1195	cell-specific gene expression	1167:1195	cell-specific gene expression of hydrolases	1167:1209	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	2	64	theme	apple	553:557	arg1	development					565:575	apple fruit development	553:575	apple fruit development	553:575	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	5	65	theme	transcriptomic	1002:1015	arg1	differences					1017:1027	transcriptomic differences	1002:1027	transcriptomic differences controlling cell type-specific wall modification	1002:1076	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	5	66	theme	RNA	956:958	arg1	RNA-seq					972:978	RNA-seq	972:978	RNA-seq	972:978	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	5	66	theme	RNA	956:958	arg1	sequencing					960:969	RNA sequencing	956:969	RNA sequencing (RNA-seq)	956:979	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	3	67	theme	cell	820:823	arg1	types					825:829	all cell types	816:829	all cell types	816:829	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	6	68	theme	hydrolases	1200:1209	arg1	expression					1186:1195	cell-specific gene expression	1167:1195	cell-specific gene expression of hydrolases	1167:1209	The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases.
31408160	1	69	dep	exocarp	193:199	arg1	epidermis					211:219	epidermis	211:219	epidermis	211:219	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	1	69	dep	exocarp	193:199	arg1	hypodermis					226:235	hypodermis	226:235	hypodermis	226:235	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	1	69	dep	exocarp	193:199	arg1	cuticle					202:208	cuticle	202:208	cuticle	202:208	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	5	70	used	used	986:989	arg2	microdissection					936:950	Laser capture microdissection	922:950	Laser capture microdissection	922:950	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	5	70	used	used	986:989	arg2	RNA-seq					972:978	RNA-seq	972:978	RNA-seq	972:978	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	5	70	used	used	986:989	arg2	sequencing					960:969	RNA sequencing	956:969	RNA sequencing (RNA-seq)	956:979	Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification.
31408160	3	71	dep	-β-d-galactan	618:630	arg1	associated					633:642	associated	633:642	associated with rigidity	633:656	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	0	72	theme	pectin	70:75	arg1	remodelling					45:55	remodelling	45:55	remodelling of cell wall pectin	45:75	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	2	73	from	composition	505:515	arg1	exocarp					524:530	the exocarp	520:530	the exocarp	520:530	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	1	74	theme	apple	134:138	arg1	fruit					158:162	apple (Malus×domestica) fruit	134:162	apple (Malus×domestica) fruit	134:162	In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth.
31408160	3	75	theme	epidermal	755:763	arg1	walls					770:774	the epidermal cell walls	751:774	the epidermal cell walls in expanding fruit	751:793	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	0	76	theme	wall	65:68	arg1	pectin					70:75	cell wall pectin	60:75	cell wall pectin	60:75	Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development.
31408160	3	77	theme	pectic	606:611	arg1	-β-d-galactan					618:630	pectic (1→4)-β-d-galactan (associated with rigidity)	606:657	pectic (1→4)-β-d-galactan (associated with rigidity)	606:657	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	3	78	theme	1→5	676:678	arg1	-α-l-arabinan					680:692	linear (1→5)-α-l-arabinan	668:692	linear (1→5)-α-l-arabinan (associated with flexibility)	668:722	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31408160	2	79	theme	epitope	427:433	arg1	chromatography					445:458	in situ immunolocalization and size exclusion epitope detection chromatography	381:458	chromatography	445:458	Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development.
31408160	8	80	theme	cell	1628:1631	arg1	remodelling					1645:1655	cell wall pectin remodelling	1628:1655	cell wall pectin remodelling	1628:1655	Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling.
31408160	3	81	theme	cell	765:768	arg1	walls					770:774	the epidermal cell walls	751:774	the epidermal cell walls in expanding fruit	751:793	Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening.
31410590	9	0	theme	wall	1610:1613	arg1	distinct					1709:1716	distinct	1709:1716	distinct	1709:1716	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	9	0	theme	wall	1610:1613	arg1	features					1615:1622	the protoplast and cell wall features	1586:1622	the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls	1586:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	6	1	theme	Eriophyidae	1083:1093	arg1	galls					1095:1099	Eriophyidae galls	1083:1099	Eriophyidae galls	1083:1099	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	5	2	from	proplastids	953:963	arg1	membranes					1023:1031	cell membranes	1018:1031	cell membranes	1018:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	3	3	theme	porous	548:553	arg1	walls					560:564	rigid and porous cell walls	538:564	rigid and porous cell walls	538:564	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	9	4	theme	cells	1631:1635	arg1	distinct					1709:1716	distinct	1709:1716	distinct	1709:1716	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	9	4	theme	cells	1631:1635	arg1	features					1615:1622	the protoplast and cell wall features	1586:1622	the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls	1586:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	3	5	theme	rigid	538:542	arg1	walls					560:564	rigid and porous cell walls	538:564	rigid and porous cell walls	538:564	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	9	6	theme	nematode	1640:1647	arg1	galls					1649:1653	nematode galls	1640:1653	nematode galls	1640:1653	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	5	7	theme	promeristematic	855:869	arg1	cells					885:889	promeristematic and secretory cells	855:889	promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes	855:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	7	8	theme	more	1356:1359	arg1	transport					1361:1369	more transport	1356:1369	more transport	1356:1369	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	5	9	from	bodies	1008:1013	arg1	membranes					1023:1031	cell membranes	1018:1031	cell membranes	1018:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	1	10	theme	Cell	121:124	arg1	walls					126:130	Cell walls	121:130	Cell walls	121:130	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31410590	7	11	theme	nutrients	1341:1349	arg1	production					1327:1336	the less production	1318:1336	the less production of nutrients	1318:1349	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	3	12	theme	pectins	594:600	arg1	composition					579:589	their composition	573:589	their composition of pectins	573:600	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	1	13	theme	functional	207:216	arg1	profiles					218:225	the functional profiles	203:225	the functional profiles of gall tissue compartments	203:253	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31410590	9	14	theme	cells	1674:1678	arg1	distinct					1709:1716	distinct	1709:1716	distinct	1709:1716	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	9	14	theme	cells	1674:1678	arg1	features					1615:1622	the protoplast and cell wall features	1586:1622	the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls	1586:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	8	15	theme	cells	1431:1435	arg1	profiles					1415:1422	The ultrastructural and immunocytochemical profiles	1372:1422	The ultrastructural and immunocytochemical profiles of CST cells	1372:1435	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	5	16	theme	enriched	907:914	arg1	cytoplasm					916:924	their enriched cytoplasm	901:924	their enriched cytoplasm	901:924	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	8	17	theme	functional	1444:1453	arg1	similarities					1455:1466	functional similarities	1444:1466	functional similarities	1444:1466	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	9	18	theme	galls	1699:1703	arg1	cells					1674:1678	the feeding cells	1662:1678	the feeding cells of the Eriophyidae galls	1662:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	5	19	from	tissues	779:785	arg1	galls					797:801	galls	797:801	galls induced by Ditylenchus gallaeformans	797:838	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	5	20	theme	secretory	875:883	arg1	cells					885:889	promeristematic and secretory cells	855:889	promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes	855:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	6	21	from	TNT	1172:1174	arg1	galls					1188:1192	nematode galls	1179:1192	nematode galls	1179:1192	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	3	22	theme	common	371:376	arg1	CSTs					395:398	The common storage tissues (CSTs)	367:399	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	367:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	7	23	theme	less	1322:1325	arg1	production					1327:1336	the less production	1318:1336	the less production of nutrients	1318:1349	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	8	24	theme	taxa	1498:1501	arg1	independent					1479:1489	independent	1479:1489	independent	1479:1489	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	0	25	theme	functional	100:109	arg1	insights					111:118	hemicellulose functional insights	86:118	hemicellulose functional insights	86:118	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	9	26	theme	gall	1776:1779	arg1	site					1795:1798	gall developmental site active	1776:1805	gall developmental site active	1776:1805	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	5	27	from	cytoplasm	916:924	arg1	membranes					1023:1031	cell membranes	1018:1031	cell membranes	1018:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	6	28	theme	feeding	1066:1072	arg1	cells					1074:1078	the feeding cells	1062:1078	the feeding cells of Eriophyidae galls	1062:1099	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	9	29	theme	different	1740:1748	arg1	strategies					1750:1759	different strategies	1740:1759	different strategies	1740:1759	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	0	30	theme	Cytological	0:10	arg1	attributes					12:21	Cytological attributes	0:21	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.	0:119	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	7	31	theme	TNT	1292:1294	arg1	cells					1279:1283	the cells	1275:1283	the cells of the TNT, which can compensate the less production of nutrients with more transport	1275:1369	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	3	32	theme	tissues	386:392	arg1	CSTs					395:398	The common storage tissues (CSTs)	367:399	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	367:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	8	33	theme	inducer	1515:1521	arg1	taxa					1498:1501	the taxa	1494:1501	the taxa of the gall inducer or of the host plant	1494:1542	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	4	34	theme	rigid	737:741	arg1	gels					751:754	rigid pectate gels	737:754	rigid pectate gels	737:754	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	4	35	dep	control	687:693	arg1	leaves					695:700	leaves	695:700	leaves	695:700	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	6	36	theme	cytological	1038:1048	arg1	features					1050:1057	The cytological features	1034:1057	The cytological features of the feeding cells of Eriophyidae galls	1034:1099	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	3	37	theme	galls	409:413	arg1	CSTs					395:398	The common storage tissues (CSTs)	367:399	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	367:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	0	38	theme	tissues	34:40	arg1	attributes					12:21	Cytological attributes	0:21	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.	0:119	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	5	39	theme	nutritive	769:777	arg1	tissues					779:785	The typical nutritive tissues	757:785	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans	757:838	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	5	39	theme	nutritive	769:777	arg1	similar					844:850	similar	844:850	similar	844:850	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	5	39	theme	nutritive	769:777	arg1	TNTs					788:791	TNTs	788:791	TNTs	788:791	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	0	40	dep	nematode	45:52	arg1	galls					68:72	galls	68:72	galls	68:72	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	8	41	theme	host	1533:1536	arg1	plant					1538:1542	the host plant	1529:1542	the host plant	1529:1542	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	9	42	theme	analogous	1559:1567	arg1	functionalities					1569:1583	their analogous functionalities	1553:1583	their analogous functionalities	1553:1583	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	3	43	theme	Ditylenchus	474:484	arg1	gallaeformans					486:498	Ditylenchus gallaeformans	474:498	Ditylenchus gallaeformans on M. ibaguensis and M. albicans	474:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	1	44	theme	profiles	218:225	arg1	establishment					186:198	the establishment	182:198	the establishment of the functional profiles of gall tissue compartments	182:253	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31410590	9	45	theme	protoplast	1590:1599	arg1	distinct					1709:1716	distinct	1709:1716	distinct	1709:1716	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	9	45	theme	protoplast	1590:1599	arg1	features					1615:1622	the protoplast and cell wall features	1586:1622	the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls	1586:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	3	46	from	Eriophyidae	426:436	arg1	ibaguensis					449:458	Miconia ibaguensis	441:458	Miconia ibaguensis leaves	441:465	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	5	47	from	mitochondria	935:946	arg1	membranes					1023:1031	cell membranes	1018:1031	cell membranes	1018:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	1	48	theme	gall	230:233	arg1	compartments					242:253	gall tissue compartments	230:253	gall tissue compartments	230:253	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31410590	9	49	theme	cell	1605:1608	arg1	wall					1610:1613	cell wall	1605:1613	cell wall	1605:1613	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	6	50	theme	galls	1095:1099	arg1	cells					1074:1078	the feeding cells	1062:1078	the feeding cells of Eriophyidae galls	1062:1099	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	3	51	contain	have	533:536	arg1	CSTs					395:398	The common storage tissues (CSTs)	367:399	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	367:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	3	51	contain	have	533:536	arg2	walls					560:564	rigid and porous cell walls	538:564	rigid and porous cell walls	538:564	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	1	52	theme	compartments	242:253	arg1	profiles					218:225	the functional profiles	203:225	the functional profiles of gall tissue compartments	203:253	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31410590	7	53	theme	wall	1215:1218	arg1	composition					1220:1230	their cell wall composition	1204:1230	their cell wall composition	1204:1230	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	9	54	theme	TNT	1627:1629	arg1	cells					1631:1635	TNT cells	1627:1635	TNT cells of nematode galls	1627:1653	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	9	55	theme	active	1800:1805	arg1	site					1795:1798	gall developmental site active	1776:1805	gall developmental site active	1776:1805	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	3	56	from	gallaeformans	486:498	arg1	ibaguensis					506:515	M. ibaguensis	503:515	M. ibaguensis	503:515	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	3	56	from	gallaeformans	486:498	arg1	albicans					524:531	M. albicans	521:531	M. albicans	521:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	9	57	theme	galls	1649:1653	arg1	cells					1631:1635	TNT cells	1627:1635	TNT cells of nematode galls	1627:1653	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	3	58	theme	cell	555:558	arg1	walls					560:564	rigid and porous cell walls	538:564	rigid and porous cell walls	538:564	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	8	59	theme	CST	1427:1429	arg1	cells					1431:1435	CST cells	1427:1435	CST cells	1427:1435	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	4	60	theme	cell	625:628	arg1	walls					630:634	CST cell walls	621:634	CST cell walls	621:634	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	5	61	theme	Ditylenchus	814:824	arg1	gallaeformans					826:838	Ditylenchus gallaeformans	814:838	Ditylenchus gallaeformans	814:838	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	9	62	theme	feeding	1666:1672	arg1	cells					1674:1678	the feeding cells	1662:1678	the feeding cells of the Eriophyidae galls	1662:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	4	63	theme	control	687:693	arg1	walls					674:678	the cell walls	665:678	the cell walls of the control leaves	665:700	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	2	64	theme	gall	302:305	arg1	systems					307:313	three gall systems	296:313	three gall systems	296:313	This presumption is herein evaluated in three gall systems by immunocytochemical and ultrastructural analyses.
31410590	9	65	theme	Eriophyidae	1687:1697	arg1	galls					1699:1703	the Eriophyidae galls	1683:1703	the Eriophyidae galls	1683:1703	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	6	66	theme	nematode	1179:1186	arg1	galls					1188:1192	nematode galls	1179:1192	nematode galls	1179:1192	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	6	67	theme	TNT	1172:1174	arg1	cells					1159:1163	the cells	1155:1163	the cells of the TNT in nematode galls	1155:1192	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	4	68	theme	cell	669:672	arg1	walls					674:678	the cell walls	665:678	the cell walls of the control leaves	665:700	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	4	69	from	Hemicelluloses	603:616	arg1	walls					630:634	CST cell walls	621:634	CST cell walls	621:634	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	0	70	from	attributes	12:21	arg1	eriophyid					58:66	eriophyid	58:66	eriophyid	58:66	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	0	70	from	attributes	12:21	arg1	nematode					45:52	nematode	45:52	nematode	45:52	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	6	71	from	galls	1188:1192	arg1	cells					1159:1163	the cells	1155:1163	the cells of the TNT in nematode galls	1155:1192	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	0	72	theme	hemicellulose	86:98	arg1	insights					111:118	hemicellulose functional insights	86:118	hemicellulose functional insights	86:118	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	5	73	theme	multivesicular	977:990	arg1	bodies					1008:1013	multivesicular and prolamellar bodies	977:1013	bodies	1008:1013	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	3	74	theme	storage	378:384	arg1	CSTs					395:398	The common storage tissues (CSTs)	367:399	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	367:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	4	75	theme	pectate	743:749	arg1	gels					751:754	rigid pectate gels	737:754	rigid pectate gels	737:754	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	6	76	from	cells	1159:1163	arg1	galls					1188:1192	nematode galls	1179:1192	nematode galls	1179:1192	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	6	77	theme	cells	1074:1078	arg1	features					1050:1057	The cytological features	1034:1057	The cytological features of the feeding cells of Eriophyidae galls	1034:1099	The cytological features of the feeding cells of Eriophyidae galls indicate that they are not as metabolically active as the cells of the TNT in nematode galls.
31410590	8	78	theme	gall	1510:1513	arg1	inducer					1515:1521	the gall inducer	1506:1521	the gall inducer	1506:1521	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	3	79	theme	Miconia	441:447	arg1	ibaguensis					449:458	Miconia ibaguensis	441:458	Miconia ibaguensis leaves	441:465	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	5	80	theme	several	927:933	arg1	mitochondria					935:946	several mitochondria	927:946	several mitochondria	927:946	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	0	81	theme	storage	26:32	arg1	tissues					34:40	storage tissues	26:40	storage tissues	26:40	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	5	82	theme	typical	761:767	arg1	tissues					779:785	The typical nutritive tissues	757:785	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans	757:838	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	5	82	theme	typical	761:767	arg1	similar					844:850	similar	844:850	similar	844:850	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	5	82	theme	typical	761:767	arg1	TNTs					788:791	TNTs	788:791	TNTs	788:791	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	9	83	theme	developmental	1781:1793	arg1	site					1795:1798	gall developmental site active	1776:1805	gall developmental site active	1776:1805	Despite their analogous functionalities, the protoplast and cell wall features of TNT cells of nematode galls and of the feeding cells of the Eriophyidae galls are distinct, and work out through different strategies toward keeping gall developmental site active.
31410590	8	84	theme	plant	1538:1542	arg1	taxa					1498:1501	the taxa	1494:1501	the taxa of the gall inducer or of the host plant	1494:1542	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	5	85	theme	cell	1018:1021	arg1	membranes					1023:1031	cell membranes	1018:1031	cell membranes	1018:1031	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	3	86	theme	leaf	404:407	arg1	galls					409:413	leaf galls	404:413	leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans	404:531	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	2	87	theme	ultrastructural	341:355	arg1	analyses					357:364	ultrastructural analyses	341:364	ultrastructural analyses	341:364	This presumption is herein evaluated in three gall systems by immunocytochemical and ultrastructural analyses.
31410590	5	88	theme	prolamellar	996:1006	arg1	bodies					1008:1013	multivesicular and prolamellar bodies	977:1013	bodies	1008:1013	The typical nutritive tissues (TNTs) in galls induced by Ditylenchus gallaeformans are similar to promeristematic and secretory cells regarding their enriched cytoplasm, several mitochondria, and proplastids, as well as multivesicular and prolamellar bodies in cell membranes.
31410590	3	89	dep	ibaguensis	449:458	arg1	leaves					460:465	leaves	460:465	leaves	460:465	The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on Miconia ibaguensis leaves and by Ditylenchus gallaeformans on M. ibaguensis and M. albicans have rigid and porous cell walls due to their composition of pectins.
31410590	8	90	theme	ultrastructural	1376:1390	arg1	profiles					1415:1422	The ultrastructural and immunocytochemical profiles	1372:1422	The ultrastructural and immunocytochemical profiles of CST cells	1372:1435	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	0	91	dep	attributes	12:21	arg1	pectin					75:80	pectin	75:80	pectin	75:80	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	0	91	dep	attributes	12:21	arg1	insights					111:118	hemicellulose functional insights	86:118	hemicellulose functional insights	86:118	Cytological attributes of storage tissues in nematode and eriophyid galls: pectin and hemicellulose functional insights.
31410590	4	92	theme	CST	621:623	arg1	walls					630:634	CST cell walls	621:634	CST cell walls	621:634	Hemicelluloses in CST cell walls are scarcer when compared to the cell walls of the control leaves, being functionally compensated by rigid pectate gels.
31410590	8	93	theme	immunocytochemical	1396:1413	arg1	profiles					1415:1422	The ultrastructural and immunocytochemical profiles	1372:1422	The ultrastructural and immunocytochemical profiles of CST cells	1372:1435	The ultrastructural and immunocytochemical profiles of CST cells reveal functional similarities, which are independent of the taxa of the gall inducer or of the host plant.
31410590	7	94	theme	cell	1210:1213	arg1	composition					1220:1230	their cell wall composition	1204:1230	their cell wall composition	1204:1230	However, their cell wall composition suggests more plasticity and porosity than the cells of the TNT, which can compensate the less production of nutrients with more transport.
31410590	1	95	theme	tissue	235:240	arg1	compartments					242:253	gall tissue compartments	230:253	gall tissue compartments	230:253	Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments.
31852406	0	0	theme	gut	78:80	arg1	microbiota					82:91	rat gut microbiota	74:91	rat gut microbiota	74:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	0	1	from	effect	11:16	arg1	production					60:69	tyrosine metabolite production	40:69	tyrosine metabolite production in rat gut microbiota	40:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	2	2	theme	significant	321:331	arg1	increase					333:340	a significant increase	319:340	a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD	319:469	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	4	3	dep	p-cresol	890:897	arg1	putrefactive					900:911	putrefactive	900:911	putrefactive	900:911	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	1	4	from	production	275:284	arg1	rats					301:304	IMD-treated rats	289:304	IMD-treated rats	289:304	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	1	5	theme	tyrosine	238:245	arg1	test					252:255	a tyrosine load test	236:255	a tyrosine load test	236:255	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	0	6	from	production	60:69	arg1	microbiota					82:91	rat gut microbiota	74:91	rat gut microbiota	74:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	1	7	theme	load	247:250	arg1	test					252:255	a tyrosine load test	236:255	a tyrosine load test	236:255	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	1	8	theme	soluble	145:151	arg1	fiber					161:165	a soluble dietary fiber	143:165	a soluble dietary fiber	143:165	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	1	8	theme	soluble	145:151	arg1	isomaltodextrin					120:134	isomaltodextrin	120:134	isomaltodextrin (IMD)	120:140	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	9	theme	products	913:920	arg1	production					866:875	production	866:875	production of phenol or p-cresol, putrefactive products in the intestine	866:937	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	2	10	from	increase	333:340	arg1	concentrations					365:378	p-cresol concentrations	356:378	p-cresol concentrations	356:378	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	2	10	from	increase	333:340	arg1	rats					383:386	rats	383:386	rats administered with only tyrosine	383:418	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	2	10	from	increase	333:340	arg1	phenol					345:350	phenol	345:350	phenol	345:350	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	3	11	theme	effect	507:512	arg1	mechanism					489:497	the mechanism	485:497	the mechanism of this effect	485:512	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	1	12	theme	dietary	153:159	arg1	fiber					161:165	a soluble dietary fiber	143:165	a soluble dietary fiber	143:165	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	1	12	theme	dietary	153:159	arg1	isomaltodextrin					120:134	isomaltodextrin	120:134	isomaltodextrin (IMD)	120:140	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	13	theme	metabolic	811:819	arg1	capacity					821:828	the metabolic capacity	807:828	the metabolic capacity for tyrosine	807:841	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	3	14	theme	intestinal	531:540	arg1	microbiota					542:551	the intestinal microbiota	527:551	the intestinal microbiota	527:551	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	2	15	theme	p-cresol	356:363	arg1	concentrations					365:378	p-cresol concentrations	356:378	p-cresol concentrations	356:378	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	0	16	theme	Inhibitory	0:9	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota	0:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	1	17	from	effect	110:115	arg1	production					171:180	production	171:180	production of putrefactive products	171:205	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	0	18	theme	isomaltodextrin	21:35	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota	0:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	4	19	theme	phenol	880:885	arg1	production					866:875	production	866:875	production of phenol or p-cresol, putrefactive products in the intestine	866:937	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	2	20	theme	only	406:409	arg1	tyrosine					411:418	only tyrosine	406:418	only tyrosine	406:418	We observed a significant increase in phenol and p-cresol concentrations in rats administered with only tyrosine, but not for rats co-administered tyrosine and IMD.
31852406	3	21	theme	direct	602:607	arg1	effect					609:614	no direct effect	599:614	no direct effect	599:614	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	3	22	contain	had	595:597	arg2	effect					609:614	no direct effect	599:614	no direct effect	599:614	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	3	22	contain	had	595:597	arg1	IMD					591:593	IMD	591:593	IMD	591:593	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	0	23	theme	tyrosine	40:47	arg1	production					60:69	tyrosine metabolite production	40:69	tyrosine metabolite production in rat gut microbiota	40:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	1	24	theme	phenol	268:273	arg1	production					275:284	phenol production	268:284	phenol production in IMD-treated rats	268:304	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	25	theme	p-cresol	890:897	arg1	products					913:920	p-cresol, putrefactive products	890:920	p-cresol, putrefactive products	890:920	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	3	26	theme	intestinal	700:709	arg1	microbiota					711:720	intestinal microbiota	700:720	intestinal microbiota	700:720	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	1	27	theme	putrefactive	185:196	arg1	products					198:205	putrefactive products	185:205	putrefactive products	185:205	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	28	theme	microbiota	776:785	arg1	composition					787:797	the intestinal microbiota composition	761:797	the intestinal microbiota composition	761:797	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	0	29	theme	metabolite	49:58	arg1	production					60:69	tyrosine metabolite production	40:69	tyrosine metabolite production in rat gut microbiota	40:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	1	30	theme	IMD-treated	289:299	arg1	rats					301:304	IMD-treated rats	289:304	IMD-treated rats	289:304	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	31	from	production	866:875	arg1	intestine					929:937	the intestine	925:937	the intestine	925:937	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	4	32	theme	intestinal	765:774	arg1	composition					787:797	the intestinal microbiota composition	761:797	the intestinal microbiota composition	761:797	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	3	33	theme	microbiota	711:720	arg1	balance					689:695	the balance	685:695	the balance of intestinal microbiota	685:720	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	3	34	theme	bacteria	637:644	arg1	proportion					623:632	the proportion	619:632	the proportion of bacteria known to produce phenols	619:669	To elucidate the mechanism of this effect, we analyzed the intestinal microbiota in each group and found that although IMD had no direct effect on the proportion of bacteria known to produce phenols, it did alter the balance of intestinal microbiota.
31852406	1	35	theme	isomaltodextrin	120:134	arg1	effect					110:115	the effect	106:115	the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products	106:205	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	1	36	theme	products	198:205	arg1	production					171:180	production	171:180	production of putrefactive products	171:205	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31852406	4	37	from	changes	750:756	arg1	composition					787:797	the intestinal microbiota composition	761:797	the intestinal microbiota composition	761:797	The results suggested that changes in the intestinal microbiota composition reduced the metabolic capacity for tyrosine and in turn suppressed production of phenol or p-cresol, putrefactive products in the intestine.
31852406	0	38	theme	rat	74:76	arg1	microbiota					82:91	rat gut microbiota	74:91	rat gut microbiota	74:91	Inhibitory effect of isomaltodextrin on tyrosine metabolite production in rat gut microbiota.
31852406	1	39	theme	intestinal	210:219	arg1	bacteria					221:228	intestinal bacteria	210:228	intestinal bacteria	210:228	We examined the effect of isomaltodextrin (IMD), a soluble dietary fiber, on production of putrefactive products by intestinal bacteria using a tyrosine load test to measure phenol production in IMD-treated rats.
31372685	5	0	theme	polysaccharides	778:792	arg1	producers					756:764	distinct producers	747:764	distinct producers of adhesive polysaccharides	747:792	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	10	1	theme	major	1559:1563	arg1	producers					1575:1583	the major potential producers	1555:1583	the major potential producers of adhesive polysaccharides	1555:1611	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	10	1	theme	major	1559:1563	arg1	Cyanobacteria					1658:1670	Cyanobacteria	1658:1670	Cyanobacteria	1658:1670	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	10	2	theme	investigated	1466:1477	arg1	genes					1479:1483	the investigated genes	1462:1483	the investigated genes	1462:1483	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	10	3	from	Cyanobacteria	1658:1670	arg1	biocrusts					1544:1552	the biocrusts	1540:1552	the biocrusts	1540:1552	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	11	4	theme	biocrust	1939:1946	arg1	development					1948:1958	biocrust development	1939:1958	biocrust development	1939:1958	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	11	5	from	shifts	1890:1895	arg1	composition					1920:1930	bacterial community composition	1900:1930	bacterial community composition	1900:1930	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	9	6	theme	soil	1322:1325	arg1	producers					1333:1341	potential "soil glue" producers	1311:1341	potential "soil glue" producers that were highly similar in bulk soils	1311:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	6	7	theme	microcosm	831:839	arg1	experiment					841:850	a microcosm experiment	829:850	a microcosm experiment	829:850	To investigate this, we performed a microcosm experiment, cultivating biocrusts on two soil substrates.
31372685	9	8	theme	same	1285:1288	arg1	time					1290:1293	the same time	1281:1293	the same time	1281:1293	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	11	9	theme	initial	1842:1848	arg1	biocrusts					1850:1858	initial biocrusts	1842:1858	initial biocrusts	1842:1858	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	5	10	theme	biocrusts	730:738	arg1	types					713:717	different types	703:717	different types of initial biocrusts	703:738	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	9	11	theme	glue	1327:1330	arg1	producers					1333:1341	potential "soil glue" producers	1311:1341	potential "soil glue" producers that were highly similar in bulk soils	1311:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	9	12	from	soils	1376:1380	arg1	similar					1360:1366	similar	1360:1366	similar	1360:1366	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	7	13	theme	High-throughput	899:913	arg1	sequencing					923:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	11	14	theme	bacterial	1822:1830	arg1	trait					1832:1836	an important bacterial trait	1809:1836	an important bacterial trait for initial biocrusts	1809:1858	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	11	14	theme	bacterial	1822:1830	arg1	potential					1745:1753	the potential to form exopolysaccharides and lipopolysaccharides	1741:1804	the potential to form exopolysaccharides and lipopolysaccharides	1741:1804	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	10	15	theme	polysaccharides	1597:1611	arg1	producers					1575:1583	the major potential producers	1555:1583	the major potential producers of adhesive polysaccharides	1555:1611	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	10	15	theme	polysaccharides	1597:1611	arg1	Cyanobacteria					1658:1670	Cyanobacteria	1658:1670	Cyanobacteria	1658:1670	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	9	16	theme	"	1331:1331	arg1	producers					1333:1341	potential "soil glue" producers	1311:1341	potential "soil glue" producers that were highly similar in bulk soils	1311:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	6	17	from	biocrusts	865:873	arg1	substrates					887:896	two soil substrates	878:896	two soil substrates	878:896	To investigate this, we performed a microcosm experiment, cultivating biocrusts on two soil substrates.
31372685	5	18	theme	distinct	747:754	arg1	producers					756:764	distinct producers	747:764	distinct producers of adhesive polysaccharides	747:792	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	0	19	from	Substrates	43:52	arg1	Crusts					16:21	Biological Soil Crusts	0:21	Biological Soil Crusts from Different Soil Substrates	0:52	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	4	20	theme	"	625:625	arg1	producers					627:635	"soil glue" producers	615:635	"soil glue" producers	615:635	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
31372685	8	21	theme	exopolysaccharides	1186:1203	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of exopolysaccharides and lipopolysaccharides	1166:1227	We discovered that the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides increased in biocrusts compared with bulk soils.
31372685	10	22	theme	bulk	1450:1453	arg1	soils					1455:1459	the bulk soils	1446:1459	the bulk soils	1446:1459	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	3	23	theme	soil	552:555	arg1	particles					557:565	soil particles	552:565	soil particles	552:565	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	3	24	theme	soil	457:460	arg1	aggregates					462:471	soil aggregates	457:471	soil aggregates	457:471	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	0	25	theme	Soil	11:14	arg1	Crusts					16:21	Biological Soil Crusts	0:21	Biological Soil Crusts from Different Soil Substrates	0:52	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	9	26	theme	bulk	1371:1374	arg1	soils					1376:1380	bulk soils	1371:1380	bulk soils	1371:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	9	27	theme	producers	1333:1341	arg1	communities					1296:1306	communities	1296:1306	communities of potential "soil glue" producers that were highly similar in bulk soils	1296:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	4	28	theme	biocrusts	671:679	arg1	development					656:666	the initial development	644:666	the initial development of biocrusts	644:679	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
31372685	0	29	theme	Biological	0:9	arg1	Crusts					16:21	Biological Soil Crusts	0:21	Biological Soil Crusts from Different Soil Substrates	0:52	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	9	30	from	similar	1360:1366	arg1	soils					1376:1380	bulk soils	1371:1380	bulk soils	1371:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	7	31	theme	metagenomic	953:963	arg1	information					965:975	metagenomic information	953:975	metagenomic information on microbiomes of bulk soils	953:1004	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	2	32	theme	initial	324:330	arg1	development					332:342	initial development	324:342	initial development	324:342	During initial development, biocrusts are dominated by bacteria.
31372685	3	33	theme	bacterial	387:395	arg1	members					397:403	Some bacterial members	382:403	Some bacterial members of the biocrusts	382:420	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	5	34	theme	different	703:711	arg1	types					713:717	different types	703:717	different types of initial biocrusts	703:738	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	7	35	theme	bulk	995:998	arg1	soils					1000:1004	bulk soils	995:1004	bulk soils	995:1004	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	7	36	theme	soils	1000:1004	arg1	microbiomes					980:990	microbiomes	980:990	microbiomes of bulk soils	980:1004	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	1	37	theme	important	204:212	arg1	role					214:217	an important role	201:217	an important role	201:217	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	10	38	dep	harbored	1490:1497	arg1	whereas					1529:1535	whereas	1529:1535	whereas	1529:1535	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	1	39	theme	soil	301:304	arg1	particles					306:314	soil particles	301:314	soil particles	301:314	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	7	40	theme	incubation	1091:1100	arg1	months					1081:1086	4 and 10 months	1072:1086	4 and 10 months of incubation	1072:1100	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	10	41	theme	potential	1565:1573	arg1	producers					1575:1583	the major potential producers	1555:1583	the major potential producers of adhesive polysaccharides	1555:1611	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	10	41	theme	potential	1565:1573	arg1	Cyanobacteria					1658:1670	Cyanobacteria	1658:1670	Cyanobacteria	1658:1670	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	1	42	theme	Biological	161:170	arg1	biocrusts					185:193	biocrusts	185:193	biocrusts	185:193	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	1	42	theme	Biological	161:170	arg1	crusts					177:182	Biological soil crusts	161:182	Biological soil crusts (biocrusts)	161:194	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	0	43	theme	Soil	38:41	arg1	Substrates					43:52	Different Soil Substrates	28:52	Different Soil Substrates	28:52	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	1	44	theme	particles	306:314	arg1	aggregation					286:296	aggregation	286:296	aggregation of soil particles	286:314	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	6	45	theme	soil	882:885	arg1	substrates					887:896	two soil substrates	878:896	two soil substrates	878:896	To investigate this, we performed a microcosm experiment, cultivating biocrusts on two soil substrates.
31372685	3	46	theme	aggregates	462:471	arg1	formation					444:452	the formation	440:452	the formation of soil aggregates	440:471	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	0	47	theme	Different	28:36	arg1	Substrates					43:52	Different Soil Substrates	28:52	Different Soil Substrates	28:52	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	5	48	theme	initial	722:728	arg1	biocrusts					730:738	initial biocrusts	722:738	initial biocrusts	722:738	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	7	49	used	used	938:941	arg2	sequencing					923:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	3	50	theme	biocrusts	412:420	arg1	members					397:403	Some bacterial members	382:403	Some bacterial members of the biocrusts	382:420	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	8	51	theme	relative	1126:1133	arg1	abundance					1135:1143	the relative abundance	1122:1143	the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides	1122:1227	We discovered that the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides increased in biocrusts compared with bulk soils.
31372685	5	52	theme	adhesive	769:776	arg1	polysaccharides					778:792	adhesive polysaccharides	769:792	adhesive polysaccharides	769:792	We hypothesized that different types of initial biocrusts harbor distinct producers of adhesive polysaccharides.
31372685	10	53	from	Chloroflexi	1675:1685	arg1	biocrusts					1544:1552	the biocrusts	1540:1552	the biocrusts	1540:1552	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	8	54	theme	bulk	1266:1269	arg1	soils					1271:1275	bulk soils	1266:1275	bulk soils	1266:1275	We discovered that the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides increased in biocrusts compared with bulk soils.
31372685	7	55	theme	experiment	1032:1041	arg1	beginning					1015:1023	the beginning	1011:1023	the beginning of the experiment	1011:1041	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	7	56	from	information	965:975	arg1	microbiomes					980:990	microbiomes	980:990	microbiomes of bulk soils	980:1004	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	10	57	from	Acidobacteria	1691:1703	arg1	biocrusts					1544:1552	the biocrusts	1540:1552	the biocrusts	1540:1552	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	11	58	theme	bacterial	1900:1908	arg1	composition					1920:1930	bacterial community composition	1900:1930	bacterial community composition	1900:1930	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	1	59	theme	soil	232:235	arg1	stability					237:245	soil stability	232:245	soil stability	232:245	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	8	60	theme	lipopolysaccharides	1209:1227	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of exopolysaccharides and lipopolysaccharides	1166:1227	We discovered that the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides increased in biocrusts compared with bulk soils.
31372685	11	61	theme	community	1910:1918	arg1	composition					1920:1930	bacterial community composition	1900:1930	bacterial community composition	1900:1930	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	8	62	theme	genes	1148:1152	arg1	abundance					1135:1143	the relative abundance	1122:1143	the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides	1122:1227	We discovered that the relative abundance of genes involved in the biosynthesis of exopolysaccharides and lipopolysaccharides increased in biocrusts compared with bulk soils.
31372685	11	63	theme	important	1812:1820	arg1	trait					1832:1836	an important bacterial trait	1809:1836	an important bacterial trait for initial biocrusts	1809:1858	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	11	63	theme	important	1812:1820	arg1	potential					1745:1753	the potential to form exopolysaccharides and lipopolysaccharides	1741:1804	the potential to form exopolysaccharides and lipopolysaccharides	1741:1804	Overall, our results indicate that the potential to form exopolysaccharides and lipopolysaccharides is an important bacterial trait for initial biocrusts and is maintained despite the shifts in bacterial community composition during biocrust development.
31372685	0	64	theme	Bacterial	70:78	arg1	Groups					80:85	Distinct Bacterial Groups	61:85	Distinct Bacterial Groups	61:85	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	4	65	theme	producers	627:635	arg1	dynamics					603:610	the dynamics	599:610	the dynamics of "soil glue" producers during the initial development of biocrusts	599:679	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
31372685	3	66	theme	glue	542:545	arg1	"					546:546	"glue"	541:546	"glue" for soil particles	541:565	Some bacterial members of the biocrusts can contribute to the formation of soil aggregates by producing exopolysaccharides and lipopolysaccharides that act as "glue" for soil particles.
31372685	4	67	theme	initial	648:654	arg1	development					656:666	the initial development	644:666	the initial development of biocrusts	644:679	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
31372685	4	68	theme	glue	621:624	arg1	producers					627:635	"soil glue" producers	615:635	"soil glue" producers	615:635	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
31372685	0	69	theme	Distinct	61:68	arg1	Groups					80:85	Distinct Bacterial Groups	61:85	Distinct Bacterial Groups	61:85	Biological Soil Crusts from Different Soil Substrates Harbor Distinct Bacterial Groups with the Potential to Produce Exopolysaccharides and Lipopolysaccharides.
31372685	9	70	theme	potential	1311:1319	arg1	producers					1333:1341	potential "soil glue" producers	1311:1341	potential "soil glue" producers that were highly similar in bulk soils	1311:1380	At the same time, communities of potential "soil glue" producers that were highly similar in bulk soils underwent differentiation once biocrusts started to develop.
31372685	10	71	theme	adhesive	1588:1595	arg1	polysaccharides					1597:1611	adhesive polysaccharides	1588:1611	adhesive polysaccharides	1588:1611	In the bulk soils, the investigated genes were harbored mainly by Betaproteobacteria, whereas in the biocrusts, the major potential producers of adhesive polysaccharides were, aside from Alphaproteobacteria, either Cyanobacteria or Chloroflexi and Acidobacteria.
31372685	1	72	theme	soil	172:175	arg1	biocrusts					185:193	biocrusts	185:193	biocrusts	185:193	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	1	72	theme	soil	172:175	arg1	crusts					177:182	Biological soil crusts	161:182	Biological soil crusts (biocrusts)	161:194	Biological soil crusts (biocrusts) play an important role in improving soil stability and resistance to erosion by promoting aggregation of soil particles.
31372685	7	73	theme	shotgun	915:921	arg1	sequencing					923:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing	899:932	High-throughput shotgun sequencing was used to obtain metagenomic information on microbiomes of bulk soils from the beginning of the experiment, and biocrusts sampled after 4 and 10 months of incubation.
31372685	4	74	theme	soil	616:619	arg1	producers					627:635	"soil glue" producers	615:635	"soil glue" producers	615:635	However, little is known about the dynamics of "soil glue" producers during the initial development of biocrusts.
30951779	4	0	theme	%	840:840	arg1	size					831:834	inoculum size	822:834	inoculum size of 3%	822:840	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	0	theme	%	840:840	arg1	pH 7.0					787:792	initial pH 7.0	779:792	initial pH 7.0	779:792	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	3	1	theme	purified	525:532	arg1	EPS					534:536	the purified EPS	521:536	the purified EPS	521:536	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	2	from	F1	557:558	arg1	optimization					470:481	The production optimization	455:481	The production optimization	455:481	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	2	from	F1	557:558	arg1	potentials					589:598	the in-vitro antioxidant potentials	564:598	the in-vitro antioxidant potentials	564:598	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	2	from	F1	557:558	arg1	characterization					501:516	characterization	501:516	characterization	501:516	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	2	from	F1	557:558	arg1	purification					484:495	purification	484:495	purification	484:495	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	5	3	with	sugar	959:963	arg1	ring					996:999	pyranoid ring	987:999	pyranoid ring	987:999	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	3	4	theme	EPS	534:536	arg1	optimization					470:481	The production optimization	455:481	The production optimization	455:481	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	4	theme	EPS	534:536	arg1	potentials					589:598	the in-vitro antioxidant potentials	564:598	the in-vitro antioxidant potentials	564:598	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	4	theme	EPS	534:536	arg1	characterization					501:516	characterization	501:516	characterization	501:516	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	3	4	theme	EPS	534:536	arg1	purification					484:495	purification	484:495	purification	484:495	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	5	5	theme	high	1165:1168	arg1	stability					1178:1186	a high thermal stability	1163:1186	a high thermal stability	1163:1186	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	6	theme	scanning	1102:1109	arg1	observation					1137:1147	scanning electron microscope (SEM) observation	1102:1147	scanning electron microscope (SEM) observation	1102:1147	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	7	theme	cultivation	744:754	arg1	conditions					756:765	the optimal cultivation conditions	732:765	the optimal cultivation conditions	732:765	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	3	8	theme	antioxidant	577:587	arg1	potentials					589:598	the in-vitro antioxidant potentials	564:598	the in-vitro antioxidant potentials	564:598	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	5	9	theme	thermal	1170:1176	arg1	stability					1178:1186	a high thermal stability	1163:1186	a high thermal stability	1163:1186	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	0	10	theme	Production	100:109	arg1	conditions					111:120	Production conditions	100:120	Production conditions	100:120	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	10	theme	Production	100:109	arg1	polysaccharides					14:28	Extracellular polysaccharides	0:28	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.	0:163	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	4	11	theme	optimal	736:742	arg1	conditions					756:765	the optimal cultivation conditions	732:765	the optimal cultivation conditions	732:765	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	12	theme	mannose	672:678	arg1	%					670:670	8% mannose	669:678	8% mannose	669:678	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	12	theme	mannose	672:678	arg1	extract					690:696	2% yeast extract	681:696	2% yeast extract	681:696	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	12	theme	mannose	672:678	arg1	%					703:703	0.04%	699:703	0.04% MgSO4·7H2O	699:714	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	12	theme	mannose	672:678	arg1	%					722:722	0.005%	717:722	0.005% Vc	717:725	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	6	13	from	glucose	1399:1405	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	4	14	theme	inoculum	822:829	arg1	size					831:834	inoculum size	822:834	inoculum size of 3%	822:840	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	14	theme	inoculum	822:829	arg1	pH 7.0					787:792	initial pH 7.0	779:792	initial pH 7.0	779:792	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	5	15	theme	303.9 °C	1224:1231	arg1	temperature					1209:1219	the degradation temperature	1193:1219	the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC)	1193:1270	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	16	theme	2	681:681	arg1	%					682:682	%	682:682	%	682:682	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	5	17	dep	transform	1012:1020	arg1	infrared					1022:1029	infrared	1022:1029	transform infrared spectroscopy (FT-IR) analysis	1012:1059	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	2	18	attach	isolated	346:353	arg2	fungus					335:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	18	attach	isolated	346:353	arg1	sinensis					369:376	Angelica sinensis	360:376	Angelica sinensis planted in Dingxi, Gansu, China,	360:409	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	18	attach	isolated	346:353	arg2	F1					451:452	Alternaria tenuissima F1	429:452	Alternaria tenuissima F1	429:452	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	5	19	theme	exchange	886:893	arg1	chromatography					902:915	ion exchange column chromatography	882:915	ion exchange column chromatography	882:915	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	1	20	theme	microorganism	218:230	arg1	EPS					251:253	EPS	251:253	EPS	251:253	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	1	20	theme	microorganism	218:230	arg1	exopolysaccharide					232:248	microorganism exopolysaccharide	218:248	microorganism exopolysaccharide (EPS)	218:254	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	5	21	theme	porous	1074:1079	arg1	structure					1089:1097	a porous network structure	1072:1097	a porous network structure	1072:1097	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	22	dep	%	722:722	arg1	Vc					724:725	Vc	724:725	0.005% Vc	717:725	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	23	theme	yeast	684:688	arg1	%					670:670	8% mannose	669:678	8% mannose	669:678	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	23	theme	yeast	684:688	arg1	extract					690:696	2% yeast extract	681:696	2% yeast extract	681:696	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	0	24	theme	Extracellular	0:12	arg1	conditions					111:120	Production conditions	100:120	Production conditions	100:120	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	24	theme	Extracellular	0:12	arg1	polysaccharides					14:28	Extracellular polysaccharides	0:28	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.	0:163	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	24	theme	Extracellular	0:12	arg1	purification					123:134	purification	123:134	purification	123:134	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	1	25	theme	metabolic	287:295	arg1	pathways					297:304	metabolic pathways	287:304	metabolic pathways	287:304	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	6	26	from	rhamnose	1378:1385	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	5	27	theme	column	895:900	arg1	chromatography					902:915	ion exchange column chromatography	882:915	ion exchange column chromatography	882:915	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	28	theme	%	682:682	arg1	%					670:670	8% mannose	669:678	8% mannose	669:678	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	28	theme	%	682:682	arg1	extract					690:696	2% yeast extract	681:696	2% yeast extract	681:696	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	0	29	theme	endophytic	33:42	arg1	F1					73:74	endophytic fungus Alternaria tenuissima F1	33:74	endophytic fungus Alternaria tenuissima F1	33:74	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	7	30	theme	new	1519:1521	arg1	source					1523:1528	a new source	1517:1528	a new source of natural antioxidant	1517:1551	The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.
30951779	3	31	theme	in-vitro	568:575	arg1	potentials					589:598	the in-vitro antioxidant potentials	564:598	the in-vitro antioxidant potentials	564:598	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	2	32	theme	Alternaria	429:438	arg1	fungus					335:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	32	theme	Alternaria	429:438	arg1	F1					451:452	Alternaria tenuissima F1	429:452	Alternaria tenuissima F1	429:452	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	33	theme	tenuissima	440:449	arg1	fungus					335:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	33	theme	tenuissima	440:449	arg1	F1					451:452	Alternaria tenuissima F1	429:452	Alternaria tenuissima F1	429:452	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	6	34	theme	EPS	1306:1308	arg1	weight					1283:1288	Molecular weight	1273:1288	Molecular weight of the purified EPS	1273:1308	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	4	35	dep	showed	626:631	arg1	%					670:670	8% mannose	669:678	8% mannose	669:678	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	35	dep	showed	626:631	arg1	extract					690:696	2% yeast extract	681:696	2% yeast extract	681:696	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	35	dep	showed	626:631	arg1	%					703:703	0.04%	699:703	0.04% MgSO4·7H2O	699:714	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	35	dep	showed	626:631	arg1	%					722:722	0.005%	717:722	0.005% Vc	717:725	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	2	36	theme	EPS-producing	310:322	arg1	fungus					335:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	36	theme	EPS-producing	310:322	arg1	F1					451:452	Alternaria tenuissima F1	429:452	Alternaria tenuissima F1	429:452	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	5	37	theme	thermogravimetric	1236:1252	arg1	TG/DSC					1264:1269	TG/DSC	1264:1269	TG/DSC	1264:1269	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	37	theme	thermogravimetric	1236:1252	arg1	analysis					1254:1261	thermogravimetric analysis	1236:1261	thermogravimetric analysis (TG/DSC)	1236:1270	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	0	38	theme	Alternaria	51:60	arg1	F1					73:74	endophytic fungus Alternaria tenuissima F1	33:74	endophytic fungus Alternaria tenuissima F1	33:74	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	6	39	theme	purified	1297:1304	arg1	EPS					1306:1308	the purified EPS	1293:1308	the purified EPS	1293:1308	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	0	40	from	sinensis	90:97	arg1	conditions					111:120	Production conditions	100:120	Production conditions	100:120	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	40	from	sinensis	90:97	arg1	polysaccharides					14:28	Extracellular polysaccharides	0:28	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.	0:163	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	40	from	sinensis	90:97	arg1	purification					123:134	purification	123:134	purification	123:134	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	4	41	theme	culture	795:801	arg1	pH 7.0					787:792	initial pH 7.0	779:792	initial pH 7.0	779:792	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	41	theme	culture	795:801	arg1	30 °C					815:819	culture temperature 30 °C	795:819	culture temperature 30 °C	795:819	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	7	42	theme	scavenging	1486:1495	arg1	activity					1497:1504	strong scavenging activity	1479:1504	strong scavenging activity	1479:1504	The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.
30951779	3	43	theme	production	459:468	arg1	optimization					470:481	The production optimization	455:481	The production optimization	455:481	The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated.
30951779	0	44	theme	fungus	44:49	arg1	F1					73:74	endophytic fungus Alternaria tenuissima F1	33:74	endophytic fungus Alternaria tenuissima F1	33:74	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	5	45	theme	electron	1111:1118	arg1	observation					1137:1147	scanning electron microscope (SEM) observation	1102:1147	scanning electron microscope (SEM) observation	1102:1147	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	0	46	theme	antioxidant	141:151	arg1	properties					153:162	antioxidant properties	141:162	antioxidant properties	141:162	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	5	47	theme	pyranoid	987:994	arg1	ring					996:999	pyranoid ring	987:999	pyranoid ring	987:999	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	1	48	theme	exopolysaccharide	232:248	arg1	structure					189:197	structure	189:197	structure	189:197	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	1	48	theme	exopolysaccharide	232:248	arg1	composition					176:186	composition	176:186	composition	176:186	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	1	48	theme	exopolysaccharide	232:248	arg1	yield					169:173	yield	169:173	yield	169:173	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	1	48	theme	exopolysaccharide	232:248	arg1	biofunction					203:213	biofunction	203:213	biofunction	203:213	The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways.
30951779	0	49	theme	F1	73:74	arg1	conditions					111:120	Production conditions	100:120	Production conditions	100:120	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	49	theme	F1	73:74	arg1	polysaccharides					14:28	Extracellular polysaccharides	0:28	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.	0:163	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	49	theme	F1	73:74	arg1	purification					123:134	purification	123:134	purification	123:134	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	5	50	theme	microscope	1120:1129	arg1	observation					1137:1147	scanning electron microscope (SEM) observation	1102:1147	scanning electron microscope (SEM) observation	1102:1147	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	51	with	glycoprotein	969:980	arg1	ring					996:999	pyranoid ring	987:999	pyranoid ring	987:999	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	6	52	from	d-galactose	1412:1422	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	0	53	theme	tenuissima	62:71	arg1	F1					73:74	endophytic fungus Alternaria tenuissima F1	33:74	endophytic fungus Alternaria tenuissima F1	33:74	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	7	54	theme	strong	1479:1484	arg1	activity					1497:1504	strong scavenging activity	1479:1504	strong scavenging activity	1479:1504	The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.
30951779	4	55	theme	temperature	803:813	arg1	pH 7.0					787:792	initial pH 7.0	779:792	initial pH 7.0	779:792	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	55	theme	temperature	803:813	arg1	30 °C					815:819	culture temperature 30 °C	795:819	culture temperature 30 °C	795:819	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	5	56	theme	SEM	1132:1134	arg1	observation					1137:1147	scanning electron microscope (SEM) observation	1102:1147	scanning electron microscope (SEM) observation	1102:1147	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	6	57	theme	Molecular	1273:1281	arg1	weight					1283:1288	Molecular weight	1273:1288	Molecular weight of the purified EPS	1273:1308	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	6	58	from	acid	1372:1375	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	5	59	theme	non-reducing	946:957	arg1	sugar					959:963	a non-reducing sugar	944:963	a non-reducing sugar	944:963	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	59	theme	non-reducing	946:957	arg1	EPS					866:868	The EPS	862:868	The EPS purified by ion exchange column chromatography and gel chromatography	862:938	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	2	60	theme	endophytic	324:333	arg1	fungus					335:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus	307:340	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	2	60	theme	endophytic	324:333	arg1	F1					451:452	Alternaria tenuissima F1	429:452	Alternaria tenuissima F1	429:452	An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1.
30951779	4	61	theme	optimal	637:643	arg1	composition					652:662	the optimal medium composition	633:662	the optimal medium composition	633:662	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	5	62	theme	gel	921:923	arg1	chromatography					925:938	gel chromatography	921:938	gel chromatography	921:938	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	63	theme	FT-IR	1045:1049	arg1	analysis					1052:1059	spectroscopy (FT-IR) analysis	1031:1059	spectroscopy (FT-IR) analysis	1031:1059	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	64	theme	ion	882:884	arg1	chromatography					902:915	ion exchange column chromatography	882:915	ion exchange column chromatography	882:915	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	6	65	theme	0.45:3.02:3.25:1.0:0.95	1436:1458	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	7	66	theme	natural	1533:1539	arg1	antioxidant					1541:1551	natural antioxidant	1533:1551	natural antioxidant	1533:1551	The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.
30951779	6	67	theme	d-galacturonic	1357:1370	arg1	acid					1372:1375	d-galacturonic acid	1357:1375	d-galacturonic acid	1357:1375	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	5	68	theme	spectroscopy	1031:1042	arg1	analysis					1052:1059	spectroscopy (FT-IR) analysis	1031:1059	spectroscopy (FT-IR) analysis	1031:1059	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	0	69	dep	polysaccharides	14:28	arg1	conditions					111:120	Production conditions	100:120	Production conditions	100:120	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	69	dep	polysaccharides	14:28	arg1	properties					153:162	antioxidant properties	141:162	antioxidant properties	141:162	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	69	dep	polysaccharides	14:28	arg1	polysaccharides					14:28	Extracellular polysaccharides	0:28	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.	0:163	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	0	69	dep	polysaccharides	14:28	arg1	purification					123:134	purification	123:134	purification	123:134	Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.
30951779	7	70	theme	antioxidant	1541:1551	arg1	source					1523:1528	a new source	1517:1528	a new source of natural antioxidant	1517:1551	The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.
30951779	5	71	theme	degradation	1197:1207	arg1	temperature					1209:1219	the degradation temperature	1193:1219	the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC)	1193:1270	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	72	gly	glycoprotein	969:980	arg1	glycoprotein					969:980	glycoprotein	969:980	glycoprotein with pyranoid ring	969:999	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	5	73	theme	network	1081:1087	arg1	structure					1089:1097	a porous network structure	1072:1097	a porous network structure	1072:1097	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	74	theme	initial	779:785	arg1	size					831:834	inoculum size	822:834	inoculum size of 3%	822:840	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	74	theme	initial	779:785	arg1	pH 7.0					787:792	initial pH 7.0	779:792	initial pH 7.0	779:792	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	74	theme	initial	779:785	arg1	180 r/min					843:851	180 r/min	843:851	180 r/min for 5 d	843:859	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	4	74	theme	initial	779:785	arg1	30 °C					815:819	culture temperature 30 °C	795:819	culture temperature 30 °C	795:819	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30951779	6	75	from	d-mannose	1388:1396	arg1	ratio					1427:1431	ratio	1427:1431	ratio of 0.45:3.02:3.25:1.0:0.95	1427:1458	Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95.
30951779	5	76	dep	Fourier	1004:1010	arg1	transform					1012:1020	transform	1012:1020	transform infrared spectroscopy (FT-IR) analysis	1012:1059	The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC).
30951779	4	77	theme	medium	645:650	arg1	composition					652:662	the optimal medium composition	633:662	the optimal medium composition	633:662	Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d.
30395776	6	0	theme	acids	911:915	arg1	production					924:933	short chain fatty acids (SCFA) production	893:933	short chain fatty acids (SCFA) production	893:933	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	10	1	theme	dose	1492:1495	arg1	fructans					1509:1516	15 g/d dose inulin type fructans	1485:1516	15 g/d dose inulin type fructans in UC	1485:1522	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	11	2	with	patients	1849:1856	arg1	UC					1870:1871	active UC	1863:1871	active UC	1863:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	6	3	theme	mucosal	812:818	arg1	communities					830:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	6	4	theme	chain	899:903	arg1	SCFA					918:921	SCFA	918:921	SCFA	918:921	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	6	4	theme	chain	899:903	arg1	acids					911:915	short chain fatty acids	893:915	short chain fatty acids (SCFA) production	893:933	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	4	5	theme	active	603:608	arg1	UC					610:611	mild/moderately active UC	587:611	mild/moderately active UC	587:611	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	6	6	theme	Fecal	802:806	arg1	communities					830:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	3	7	theme	communities	551:561	arg1	shifts					490:495	compositional and/or functional shifts	458:495	compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities	458:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	8	8	theme	fecal	1195:1199	arg1	levels					1210:1215	fecal butyrate levels	1195:1215	fecal butyrate levels	1195:1215	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	2	9	theme	microbiota	288:297	arg1	composition					299:309	gut microbiota composition	284:309	gut microbiota composition	284:309	Prebiotics are hypothesized to improve health through alterations to gut microbiota composition and/or activity.
30395776	8	10	theme	butyrate	1151:1158	arg1	production					1160:1169	colonic butyrate production	1143:1169	colonic butyrate production	1143:1169	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	4	11	theme	daily	654:658	arg1	inulin					688:693	daily oral oligofructose-enriched inulin	654:693	daily oral oligofructose-enriched inulin (Orafti®Synergy1)	654:711	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	4	11	theme	daily	654:658	arg1	Orafti®Synergy1					696:710	Orafti®Synergy1	696:710	Orafti®Synergy1	696:710	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	10	12	theme	compositional	1552:1564	arg1	shifts					1566:1571	functional but not compositional shifts	1533:1571	functional but not compositional shifts of the gut microbiota	1533:1593	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	11	13	theme	controlled	1782:1791	arg1	studies					1793:1799	future well-powered controlled studies	1762:1799	future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC	1762:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	7	14	theme	clinical	1061:1068	arg1	response					1070:1077	a clinical response	1059:1077	a clinical response	1059:1077	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	9	15	theme	improved	1417:1424	arg1	scores					1434:1439	improved disease scores	1417:1439	improved disease scores	1417:1439	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	6	16	theme	fecal	952:956	arg1	samples					958:964	fecal samples	952:964	fecal samples	952:964	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	11	17	theme	future	1762:1767	arg1	studies					1793:1799	future well-powered controlled studies	1762:1799	future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC	1762:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	8	18	theme	15 g/d	1178:1183	arg1	dose					1185:1188	the 15 g/d dose	1174:1188	the 15 g/d dose	1174:1188	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	10	19	theme	gut	1580:1582	arg1	microbiota					1584:1593	the gut microbiota	1576:1593	the gut microbiota	1576:1593	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	10	20	from	benefits	1725:1732	arg1	UC					1737:1738	UC	1737:1738	UC	1737:1738	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	4	21	theme	oligofructose-enriched	665:686	arg1	inulin					688:693	daily oral oligofructose-enriched inulin	654:693	daily oral oligofructose-enriched inulin (Orafti®Synergy1)	654:711	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	4	21	theme	oligofructose-enriched	665:686	arg1	Orafti®Synergy1					696:710	Orafti®Synergy1	696:710	Orafti®Synergy1	696:710	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	5	22	theme	endoscopic	744:753	arg1	activity					755:762	endoscopic activity	744:762	endoscopic activity	744:762	Total Mayo score, endoscopic activity and fecal calprotectin were assessed.
30395776	1	23	theme	ulcerative	177:186	arg1	UC					197:198	UC	197:198	UC	197:198	The intestinal microbiota is involved in ulcerative colitis (UC) pathogenesis.
30395776	1	23	theme	ulcerative	177:186	arg1	colitis					188:194	ulcerative colitis	177:194	ulcerative colitis (UC) pathogenesis	177:212	The intestinal microbiota is involved in ulcerative colitis (UC) pathogenesis.
30395776	0	24	theme	short-chain	104:114	arg1	acids					122:126	short-chain fatty acids	104:126	increased short-chain fatty acids levels	94:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	3	25	from	benefits	403:410	arg1	UC					415:416	UC	415:416	UC	415:416	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	3	26	theme	inulin-type	366:376	arg1	fructans					378:385	inulin-type fructans	366:385	inulin-type fructans	366:385	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	10	27	theme	prebiotic-induced	1612:1628	arg1	alterations					1630:1640	prebiotic-induced alterations	1612:1640	prebiotic-induced alterations of gut microbiota metabolism	1612:1669	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	0	28	theme	acids	122:126	arg1	levels					128:133	increased short-chain fatty acids levels	94:133	increased short-chain fatty acids levels	94:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	0	29	theme	Inulin-type	0:10	arg1	fructans					12:19	Inulin-type fructans	0:19	Inulin-type fructans	0:19	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	10	30	theme	pilot	1459:1463	arg1	study					1465:1469	this pilot study	1454:1469	this pilot study	1454:1469	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	11	31	from	therapy	1838:1844	arg1	patients					1849:1856	patients	1849:1856	patients with active UC	1849:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	10	32	theme	microbiota	1649:1658	arg1	metabolism					1660:1669	gut microbiota metabolism	1645:1669	gut microbiota metabolism	1645:1669	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	9	33	theme	fructan	1293:1299	arg1	dose					1301:1304	The high fructan dose	1284:1304	The high fructan dose	1284:1304	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	4	34	with	Patients	564:571	arg1	UC					610:611	mild/moderately active UC	587:611	mild/moderately active UC	587:611	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	6	35	theme	tag	873:875	arg1	sequencing					877:886	rRNA tag sequencing	868:886	rRNA tag sequencing	868:886	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	0	36	theme	ulcerative	36:45	arg1	colitis					47:53	active ulcerative colitis	29:53	active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels	29:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	10	37	from	fructans	1509:1516	arg1	UC					1521:1522	UC	1521:1522	UC	1521:1522	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	7	38	from	%	1088:1088	arg1	P = 0.04					1113:1120	P = 0.04	1113:1120	P = 0.04	1113:1120	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	7	38	from	%	1088:1088	arg1	group					1106:1110	the low-dose group	1093:1110	the low-dose group (P = 0.04)	1093:1121	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	10	39	theme	15 g/d	1485:1490	arg1	fructans					1509:1516	15 g/d dose inulin type fructans	1485:1516	15 g/d dose inulin type fructans in UC	1485:1522	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	0	40	theme	microbiota	71:80	arg1	changes					82:88	microbiota changes	71:88	microbiota changes	71:88	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	11	41	theme	adjunct	1830:1836	arg1	therapy					1838:1844	adjunct therapy	1830:1844	adjunct therapy in patients with active UC	1830:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	11	42	theme	β-fructans	1816:1825	arg1	use					1809:1811	the use	1805:1811	the use of β-fructans as adjunct therapy in patients with active UC	1805:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	9	43	theme	Bifidobacteriaceae	1326:1343	arg1	abundance					1365:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	3	44	theme	mucosal	524:530	arg1	communities					551:561	the luminal (fecal) and mucosal (biopsy) bacterial communities	500:561	communities	551:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	9	45	theme	Lachnospiraceae	1349:1363	arg1	abundance					1365:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	10	46	theme	inulin	1497:1502	arg1	fructans					1509:1516	15 g/d dose inulin type fructans	1485:1516	15 g/d dose inulin type fructans in UC	1485:1522	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	10	47	theme	compositional	1695:1707	arg1	changes					1709:1715	compositional changes	1695:1715	compositional changes for the benefits in UC	1695:1738	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	6	48	theme	fatty	905:909	arg1	SCFA					918:921	SCFA	918:921	SCFA	918:921	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	6	48	theme	fatty	905:909	arg1	acids					911:915	short chain fatty acids	893:915	short chain fatty acids (SCFA) production	893:933	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	10	49	theme	functional	1533:1542	arg1	shifts					1566:1571	functional but not compositional shifts	1533:1571	functional but not compositional shifts of the gut microbiota	1533:1593	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	3	50	theme	bacterial	541:549	arg1	communities					551:561	the luminal (fecal) and mucosal (biopsy) bacterial communities	500:561	communities	551:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	6	51	theme	short	893:897	arg1	SCFA					918:921	SCFA	918:921	SCFA	918:921	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	6	51	theme	short	893:897	arg1	acids					911:915	short chain fatty acids	893:915	short chain fatty acids (SCFA) production	893:933	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	3	52	theme	biopsy	533:538	arg1	communities					551:561	the luminal (fecal) and mucosal (biopsy) bacterial communities	500:561	communities	551:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	1	53	theme	colitis	188:194	arg1	pathogenesis					201:212	ulcerative colitis (UC) pathogenesis	177:212	ulcerative colitis (UC) pathogenesis	177:212	The intestinal microbiota is involved in ulcerative colitis (UC) pathogenesis.
30395776	8	54	theme	colonic	1143:1149	arg1	production					1160:1169	colonic butyrate production	1143:1169	colonic butyrate production	1143:1169	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	3	55	attach	linked	448:453	arg2	benefits					435:442	benefits	435:442	benefits	435:442	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	3	55	attach	linked	448:453	arg1	shifts					490:495	compositional and/or functional shifts	458:495	compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities	458:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	8	56	theme	butyrate	1201:1208	arg1	levels					1210:1215	fecal butyrate levels	1195:1215	fecal butyrate levels	1195:1215	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	7	57	theme	patients	1042:1049	arg1	patients					1042:1049	patients	1042:1049	patients showing a clinical response	1042:1077	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	7	57	theme	patients	1042:1049	arg1	%					1037:1037	77%	1035:1037	77% of patients showing a clinical response	1035:1077	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	9	58	theme	disease	1426:1432	arg1	scores					1434:1439	improved disease scores	1417:1439	improved disease scores	1417:1439	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	10	59	theme	microbiota	1584:1593	arg1	shifts					1566:1571	functional but not compositional shifts	1533:1571	functional but not compositional shifts of the gut microbiota	1533:1593	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	11	60	theme	well-powered	1769:1780	arg1	studies					1793:1799	future well-powered controlled studies	1762:1799	future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC	1762:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
30395776	7	61	theme	high-dose	1013:1021	arg1	group					1023:1027	the high-dose group	1009:1027	the high-dose group	1009:1027	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	8	62	theme	Mayo	1249:1252	arg1	P = 0.036					1272:1280	P = 0.036	1272:1280	P = 0.036	1272:1280	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	8	62	theme	Mayo	1249:1252	arg1	score					1254:1258	Mayo score	1249:1258	Mayo score (r = -0.50; P = 0.036)	1249:1281	Fructans increased colonic butyrate production in the 15 g/d dose, and fecal butyrate levels were negatively correlated with Mayo score (r = -0.50; P = 0.036).
30395776	4	63	dep	7.5 g	622:626	arg1	inulin					688:693	daily oral oligofructose-enriched inulin	654:693	daily oral oligofructose-enriched inulin (Orafti®Synergy1)	654:711	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	4	63	dep	7.5 g	622:626	arg1	Orafti®Synergy1					696:710	Orafti®Synergy1	696:710	Orafti®Synergy1	696:710	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	2	64	theme	gut	284:286	arg1	composition					299:309	gut microbiota composition	284:309	gut microbiota composition	284:309	Prebiotics are hypothesized to improve health through alterations to gut microbiota composition and/or activity.
30395776	1	65	theme	intestinal	140:149	arg1	microbiota					151:160	The intestinal microbiota	136:160	The intestinal microbiota	136:160	The intestinal microbiota is involved in ulcerative colitis (UC) pathogenesis.
30395776	4	66	theme	oral	660:663	arg1	inulin					688:693	daily oral oligofructose-enriched inulin	654:693	daily oral oligofructose-enriched inulin (Orafti®Synergy1)	654:711	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	4	66	theme	oral	660:663	arg1	Orafti®Synergy1					696:710	Orafti®Synergy1	696:710	Orafti®Synergy1	696:710	Patients (n = 25) with mild/moderately active UC received 7.5 g (n = 12) or 15 g (n = 13) daily oral oligofructose-enriched inulin (Orafti®Synergy1) for 9 weeks.
30395776	5	67	theme	Total	726:730	arg1	score					737:741	Total Mayo score	726:741	Total Mayo score	726:741	Total Mayo score, endoscopic activity and fecal calprotectin were assessed.
30395776	0	68	theme	increased	94:102	arg1	levels					128:133	increased short-chain fatty acids levels	94:133	increased short-chain fatty acids levels	94:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	0	69	theme	fatty	116:120	arg1	acids					122:126	short-chain fatty acids	104:126	increased short-chain fatty acids levels	94:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	3	70	theme	clinical	394:401	arg1	benefits					403:410	clinical benefits	394:410	clinical benefits in UC	394:416	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	5	71	theme	Mayo	732:735	arg1	score					737:741	Total Mayo score	726:741	Total Mayo score	726:741	Total Mayo score, endoscopic activity and fecal calprotectin were assessed.
30395776	3	72	theme	compositional	458:470	arg1	shifts					490:495	compositional and/or functional shifts	458:495	compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities	458:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	0	73	theme	active	29:34	arg1	colitis					47:53	active ulcerative colitis	29:53	active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels	29:133	Inulin-type fructans improve active ulcerative colitis associated with microbiota changes and increased short-chain fatty acids levels.
30395776	9	74	theme	high	1288:1291	arg1	dose					1301:1304	The high fructan dose	1284:1304	The high fructan dose	1284:1304	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	10	75	theme	gut	1645:1647	arg1	metabolism					1660:1669	gut microbiota metabolism	1645:1669	gut microbiota metabolism	1645:1669	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	5	76	theme	fecal	768:772	arg1	calprotectin					774:785	fecal calprotectin	768:785	fecal calprotectin	768:785	Total Mayo score, endoscopic activity and fecal calprotectin were assessed.
30395776	6	77	theme	rRNA	868:871	arg1	sequencing					877:886	rRNA tag sequencing	868:886	rRNA tag sequencing	868:886	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	3	78	theme	functional	479:488	arg1	shifts					490:495	compositional and/or functional shifts	458:495	compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities	458:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	7	79	theme	low-dose	1097:1104	arg1	P = 0.04					1113:1120	P = 0.04	1113:1120	P = 0.04	1113:1120	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	7	79	theme	low-dose	1097:1104	arg1	group					1106:1110	the low-dose group	1093:1110	the low-dose group (P = 0.04)	1093:1121	Fructans significantly reduced colitis in the high-dose group, with 77% of patients showing a clinical response versus 33% in the low-dose group (P = 0.04).
30395776	3	80	theme	fecal	513:517	arg1	shifts					490:495	compositional and/or functional shifts	458:495	compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities	458:561	Our aim was therefore to determine if inulin-type fructans induce clinical benefits in UC, and identify if benefits are linked to compositional and/or functional shifts of the luminal (fecal) and mucosal (biopsy) bacterial communities.
30395776	9	81	theme	increased	1316:1324	arg1	abundance					1365:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	an increased Bifidobacteriaceae and Lachnospiraceae abundance	1313:1373	The high fructan dose led to an increased Bifidobacteriaceae and Lachnospiraceae abundance but these shifts were not correlated with improved disease scores.
30395776	10	82	theme	metabolism	1660:1669	arg1	alterations					1630:1640	prebiotic-induced alterations	1612:1640	prebiotic-induced alterations of gut microbiota metabolism	1612:1669	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	6	83	theme	bacterial	820:828	arg1	communities					830:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities	802:840	Fecal and mucosal bacterial communities were characterized by 16S rRNA tag sequencing, and short chain fatty acids (SCFA) production were measured in fecal samples.
30395776	10	84	theme	type	1504:1507	arg1	fructans					1509:1516	15 g/d dose inulin type fructans	1485:1516	15 g/d dose inulin type fructans in UC	1485:1522	In summary, this pilot study revealed that 15 g/d dose inulin type fructans in UC produced functional but not compositional shifts of the gut microbiota, suggesting that prebiotic-induced alterations of gut microbiota metabolism are more important than compositional changes for the benefits in UC.
30395776	11	85	theme	active	1863:1868	arg1	UC					1870:1871	active UC	1863:1871	active UC	1863:1871	The findings warrant future well-powered controlled studies for the use of β-fructans as adjunct therapy in patients with active UC.
29313353	0	0	theme	Polysaccharides	73:87	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry	49:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	5	1	theme	sensu	1173:1177	arg1	Bacteroides					1052:1062	genera Bacteroides	1045:1062	genera Bacteroides	1045:1062	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	1	theme	sensu	1173:1177	arg1	stricto					1179:1185	Clostridium sensu stricto	1161:1185	Clostridium sensu stricto	1161:1185	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	1	2	from	fermentation	151:162	arg1	pollen					211:216	bee collected pollen	197:216	bee collected pollen of Chinese wolfberry (WBPPS)	197:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	4	3	theme	intestinal	637:646	arg1	juices					648:653	simulated gastric and small intestinal juices	609:653	simulated gastric and small intestinal juices	609:653	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	0	4	theme	Bee	94:96	arg1	Pollen					108:113	Bee Collected Pollen	94:113	Bee Collected Pollen of Chinese Wolfberry	94:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	4	5	theme	weight	560:565	arg1	kDa					572:574	molecular weight 1340 kDa	550:574	molecular weight 1340 kDa	550:574	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	5	theme	weight	560:565	arg1	A					547:547	A	547:547	The fraction A (molecular weight 1340 kDa) of WBPPS	534:584	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	6	dep	fraction	538:545	arg1	kDa					572:574	molecular weight 1340 kDa	550:574	molecular weight 1340 kDa	550:574	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	6	dep	fraction	538:545	arg1	A					547:547	A	547:547	The fraction A (molecular weight 1340 kDa) of WBPPS	534:584	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	7	theme	weight	694:699	arg1	kDa					705:707	molecular weight 523 kDa	684:707	molecular weight 523 kDa	684:707	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	7	theme	weight	694:699	arg1	B					681:681	B	681:681	the small fraction B (molecular weight 523 kDa) of WBPPS	662:717	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	1	8	from	Digestion	137:145	arg1	pollen					211:216	bee collected pollen	197:216	bee collected pollen of Chinese wolfberry (WBPPS)	197:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	3	9	theme	human	520:524	arg1	saliva					526:531	human saliva	520:531	human saliva	520:531	WBPPS was not affected by human saliva.
29313353	4	10	theme	molecular	550:558	arg1	kDa					572:574	molecular weight 1340 kDa	550:574	molecular weight 1340 kDa	550:574	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	10	theme	molecular	550:558	arg1	A					547:547	A	547:547	The fraction A (molecular weight 1340 kDa) of WBPPS	534:584	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	5	11	theme	genera	1045:1050	arg1	Parabacteroides					1083:1097	Parabacteroides	1083:1097	Parabacteroides	1083:1097	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	11	theme	genera	1045:1050	arg1	Parasutterella					1145:1158	Parasutterella	1145:1158	Parasutterella	1145:1158	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	11	theme	genera	1045:1050	arg1	Fusobacterium					1192:1204	Fusobacterium	1192:1204	Fusobacterium	1192:1204	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	11	theme	genera	1045:1050	arg1	Bacteroides					1052:1062	genera Bacteroides	1045:1062	genera Bacteroides	1045:1062	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	11	theme	genera	1045:1050	arg1	Phascolarctobacterium					1122:1142	Phascolarctobacterium	1122:1142	Phascolarctobacterium	1122:1142	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	11	theme	genera	1045:1050	arg1	stricto					1179:1185	Clostridium sensu stricto	1161:1185	Clostridium sensu stricto	1161:1185	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	4	12	theme	WBPPS	580:584	arg1	fraction					538:545	The fraction A (molecular weight 1340 kDa)	534:575	The fraction A (molecular weight 1340 kDa) of WBPPS	534:584	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	5	13	theme	relative	914:921	arg1	Faecalibacterium					978:993	Faecalibacterium	978:993	Faecalibacterium	978:993	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	13	theme	relative	914:921	arg1	Alloprevotella					1000:1013	Alloprevotella	1000:1013	Alloprevotella	1000:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	13	theme	relative	914:921	arg1	Dialister					956:964	Dialister	956:964	Dialister	956:964	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	13	theme	relative	914:921	arg1	abundances					923:932	the relative abundances	910:932	the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella	910:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	4	14	theme	gastric	619:625	arg1	juices					648:653	simulated gastric and small intestinal juices	609:653	simulated gastric and small intestinal juices	609:653	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	0	15	theme	Collected	98:106	arg1	Pollen					108:113	Bee Collected Pollen	94:113	Bee Collected Pollen of Chinese Wolfberry	94:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	5	16	theme	Bacteroides	1052:1062	arg1	numbers					1034:1040	the numbers	1030:1040	the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium	1030:1204	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	2	17	theme	galacturonic	358:369	arg1	acid					371:374	galacturonic acid	358:374	galacturonic acid	358:374	It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively.
29313353	5	18	theme	gut	868:870	arg1	composition					883:893	gut microbiota composition	868:893	gut microbiota composition	868:893	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	19	from	fermentation	743:754	arg1	vitro					759:763	vitro	759:763	vitro	759:763	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	1	20	theme	polysaccharides	176:190	arg1	fermentation					151:162	fermentation	151:162	fermentation in vitro	151:171	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	1	20	theme	polysaccharides	176:190	arg1	Digestion					137:145	Digestion	137:145	Digestion	137:145	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	0	21	theme	Simulated	0:8	arg1	Digestion					10:18	Simulated Digestion	0:18	Simulated Digestion	0:18	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	5	22	theme	microbiota	872:881	arg1	composition					883:893	gut microbiota composition	868:893	gut microbiota composition	868:893	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	1	23	theme	present	272:278	arg1	study					280:284	the present study	268:284	the present study	268:284	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	0	24	theme	Chinese	118:124	arg1	Wolfberry					126:134	Chinese Wolfberry	118:134	Chinese Wolfberry	118:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	4	25	theme	molecular	684:692	arg1	kDa					705:707	molecular weight 523 kDa	684:707	molecular weight 523 kDa	684:707	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	25	theme	molecular	684:692	arg1	B					681:681	B	681:681	the small fraction B (molecular weight 523 kDa) of WBPPS	662:717	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	1	26	theme	bee	197:199	arg1	pollen					211:216	bee collected pollen	197:216	bee collected pollen of Chinese wolfberry (WBPPS)	197:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	5	27	theme	short-chain	831:841	arg1	acids					849:853	short-chain fatty acids	831:853	short-chain fatty acids	831:853	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	1	28	theme	collected	201:209	arg1	pollen					211:216	bee collected pollen	197:216	bee collected pollen of Chinese wolfberry (WBPPS)	197:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	2	29	theme	molar	424:428	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively	422:491	It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively.
29313353	5	30	theme	Clostridium	1161:1171	arg1	Bacteroides					1052:1062	genera Bacteroides	1045:1062	genera Bacteroides	1045:1062	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	30	theme	Clostridium	1161:1171	arg1	stricto					1179:1185	Clostridium sensu stricto	1161:1185	Clostridium sensu stricto	1161:1185	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	0	31	theme	Wolfberry	126:134	arg1	Pollen					108:113	Bee Collected Pollen	94:113	Bee Collected Pollen of Chinese Wolfberry	94:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	0	32	from	Microbiota	59:68	arg1	Pollen					108:113	Bee Collected Pollen	94:113	Bee Collected Pollen of Chinese Wolfberry	94:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	1	33	from	pollen	211:216	arg1	polysaccharides					176:190	polysaccharides	176:190	polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS)	176:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	1	33	from	pollen	211:216	arg1	fermentation					151:162	fermentation	151:162	fermentation in vitro	151:171	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	1	33	from	pollen	211:216	arg1	Digestion					137:145	Digestion	137:145	Digestion	137:145	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	0	34	theme	Human	49:53	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry	49:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	0	35	theme	Gut	55:57	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry	49:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	5	36	theme	fatty	843:847	arg1	acids					849:853	short-chain fatty acids	831:853	short-chain fatty acids	831:853	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	0	37	from	Pollen	108:113	arg1	Polysaccharides					73:87	Polysaccharides	73:87	Polysaccharides from Bee Collected Pollen of Chinese Wolfberry	73:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	0	37	from	Pollen	108:113	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry	49:134	Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry.
29313353	5	38	theme	acids	849:853	arg1	production					817:826	the production	813:826	the production of short-chain fatty acids	813:853	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	4	39	theme	WBPPS	713:717	arg1	fraction					672:679	the small fraction B (molecular weight 523 kDa)	662:708	the small fraction B (molecular weight 523 kDa) of WBPPS	662:717	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	40	theme	small	666:670	arg1	fraction					672:679	the small fraction B (molecular weight 523 kDa)	662:708	the small fraction B (molecular weight 523 kDa) of WBPPS	662:717	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	1	41	theme	Chinese	221:227	arg1	WBPPS					240:244	Chinese wolfberry (WBPPS)	221:245	Chinese wolfberry (WBPPS)	221:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	5	42	theme	genera	937:942	arg1	Faecalibacterium					978:993	Faecalibacterium	978:993	Faecalibacterium	978:993	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	42	theme	genera	937:942	arg1	Alloprevotella					1000:1013	Alloprevotella	1000:1013	Alloprevotella	1000:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	42	theme	genera	937:942	arg1	Dialister					956:964	Dialister	956:964	Dialister	956:964	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	42	theme	genera	937:942	arg1	abundances					923:932	the relative abundances	910:932	the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella	910:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	4	43	dep	fraction	672:679	arg1	kDa					705:707	molecular weight 523 kDa	684:707	molecular weight 523 kDa	684:707	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	4	43	dep	fraction	672:679	arg1	B					681:681	B	681:681	the small fraction B (molecular weight 523 kDa) of WBPPS	662:717	The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded.
29313353	1	44	theme	wolfberry	229:237	arg1	WBPPS					240:244	Chinese wolfberry (WBPPS)	221:245	Chinese wolfberry (WBPPS)	221:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
29313353	5	45	dep	abundances	923:932	arg1	Faecalibacterium					978:993	Faecalibacterium	978:993	Faecalibacterium	978:993	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	45	dep	abundances	923:932	arg1	Alloprevotella					1000:1013	Alloprevotella	1000:1013	Alloprevotella	1000:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	45	dep	abundances	923:932	arg1	Dialister					956:964	Dialister	956:964	Dialister	956:964	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	5	45	dep	abundances	923:932	arg1	abundances					923:932	the relative abundances	910:932	the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella	910:1013	Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.
29313353	2	46	theme	0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03	439:477	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively	422:491	It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively.
29313353	1	47	theme	WBPPS	240:244	arg1	pollen					211:216	bee collected pollen	197:216	bee collected pollen of Chinese wolfberry (WBPPS)	197:245	Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study.
30184795	4	0	theme	assembly	828:835	arg1	evident					853:859	evident	853:859	evident	853:859	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	4	0	theme	assembly	828:835	arg1	formation					797:805	the formation	793:805	the formation	793:805	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	7	1	theme	samples	1167:1173	arg1	composition					1144:1154	The elemental composition	1130:1154	The elemental composition of the HAP samples	1130:1173	The elemental composition of the HAP samples was evaluated by X-ray photoelectron spectroscopy.
30184795	9	2	theme	scanning	1556:1563	arg1	micrographs					1574:1584	scanning electron micrographs	1556:1584	scanning electron micrographs	1556:1584	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	9	3	theme	HAP	1477:1479	arg1	samples					1466:1472	Pellet samples	1459:1472	Pellet samples of HAP	1459:1479	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	10	4	theme	pressing	1653:1660	arg1	method					1662:1667	pellet pressing method	1646:1667	pellet pressing method	1646:1667	The drug, naringenin, was loaded within the core of HAP by pellet pressing method.
30184795	8	5	from	532.1 eV	1313:1320	arg1	peaks					1291:1295	the peaks	1287:1295	the peaks at 530.6 eV and 532.1 eV of the O 1S spectra	1287:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	5	from	532.1 eV	1313:1320	arg1	peaks					1230:1234	The peaks	1226:1234	The peaks at binding energies 286.5 and 289.3 eV of C 1S	1226:1281	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	4	6	theme	shell-HAP	813:821	arg1	assembly					828:835	GA shell-HAP core assembly	810:835	GA shell-HAP core assembly	810:835	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	6	7	theme	's	1105:1106	arg1	1.67					1124:1127	1.67	1124:1127	1.67	1124:1127	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	6	7	theme	's	1105:1106	arg1	ratio					1077:1081	The Ca/P ratio	1068:1081	The Ca/P ratio of the synthesized HAP's	1068:1106	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	3	8	theme	GA-HAP	552:557	arg1	nanocomposite					559:571	GA-HAP nanocomposite	552:571	GA-HAP nanocomposite	552:571	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	9	9	theme	electron	1565:1572	arg1	micrographs					1574:1584	scanning electron micrographs	1556:1584	scanning electron micrographs	1556:1584	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	4	10	theme	%	867:867	arg1	composites					876:885	10% GA-HAP composites	865:885	10% GA-HAP composites	865:885	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	6	11	theme	synthesized	1090:1100	arg1	's					1105:1106	the synthesized HAP's	1086:1106	the synthesized HAP's	1086:1106	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	11	12	theme	HAP	1697:1699	arg1	composites					1701:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	5	13	attach	present	920:926	arg2	groups					913:918	functional groups	902:918	functional groups present in HAP	902:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	5	13	attach	present	920:926	arg1	HAP					931:933	HAP	931:933	HAP	931:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	8	14	theme	binding	1239:1245	arg1	energies					1247:1254	binding energies 286.5 and 289.3 eV	1239:1273	energies	1247:1254	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	5	15	theme	chemical	997:1004	arg1	composition					1006:1016	its chemical composition	993:1016	its chemical composition	993:1016	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	1	16	theme	acacia	149:154	arg1	nanocomposite					161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite	111:173	nanocomposite	161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	2	17	theme	X-ray	460:464	arg1	diffraction					466:476	X-ray diffraction measurement and transmission electron microscopy	460:525	diffraction	466:476	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	8	18	theme	HAP	1392:1394	arg1	particles					1396:1404	the HAP particles	1388:1404	the HAP particles	1388:1404	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	7	19	theme	HAP	1163:1165	arg1	samples					1167:1173	the HAP samples	1159:1173	the HAP samples	1159:1173	The elemental composition of the HAP samples was evaluated by X-ray photoelectron spectroscopy.
30184795	1	20	theme	GA	157:158	arg1	nanocomposite					161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite	111:173	nanocomposite	161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	11	21	theme	drug-loaded	1674:1684	arg1	composites					1701:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	1	22	theme	2.4H2O	295:300	arg1	precursors					317:326	Ca(NO3)2.4H2O and NH4H2PO4as precursors	288:326	Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P	288:339	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	2	23	theme	transmission	494:505	arg1	microscopy					516:525	transmission electron microscopy	494:525	transmission electron microscopy	494:525	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	8	24	theme	C	1278:1278	arg1	1S					1280:1281	C 1S	1278:1281	C 1S	1278:1281	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	7	25	theme	elemental	1134:1142	arg1	composition					1144:1154	The elemental composition	1130:1154	The elemental composition of the HAP samples	1130:1173	The elemental composition of the HAP samples was evaluated by X-ray photoelectron spectroscopy.
30184795	5	26	theme	dispersive	1041:1050	arg1	analysis					1058:1065	energy dispersive X-ray analysis	1034:1065	energy dispersive X-ray analysis	1034:1065	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	5	27	theme	groups	913:918	arg1	nature					892:897	The nature	888:897	The nature of functional groups present in HAP	888:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	4	28	theme	GA	755:756	arg1	encapsulation					738:750	encapsulation	738:750	encapsulation of GA over the HAP particles	738:779	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	8	29	from	289.3 eV	1266:1273	arg1	peaks					1291:1295	the peaks	1287:1295	the peaks at 530.6 eV and 532.1 eV of the O 1S spectra	1287:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	29	from	289.3 eV	1266:1273	arg1	peaks					1230:1234	The peaks	1226:1234	The peaks at binding energies 286.5 and 289.3 eV of C 1S	1226:1281	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	30	theme	GA-HAP	1437:1442	arg1	nanocomposite					1444:1456	GA-HAP nanocomposite	1437:1456	GA-HAP nanocomposite	1437:1456	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	4	31	theme	Transmission	696:707	arg1	micrographs					718:728	Transmission electron micrographs	696:728	Transmission electron micrographs	696:728	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	0	32	theme	New	0:2	arg1	hydroxyapatite/Gum-Acacia					15:39	New core-shell hydroxyapatite/Gum-Acacia	0:39	New core-shell hydroxyapatite/Gum-Acacia	0:39	New core-shell hydroxyapatite/Gum-Acacia nanocomposites for drug delivery and tissue engineering applications.
30184795	1	33	theme	hydroxyapatite	122:135	arg1	HAP					138:140	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite	111:173	HAP	138:140	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	5	34	theme	FT-IR	956:960	arg1	spectroscopies					972:985	FT-IR and Raman spectroscopies	956:985	spectroscopies	972:985	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	2	35	dep	diffraction	466:476	arg1	measurement					478:488	measurement	478:488	measurement	478:488	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	2	36	theme	nanocomposite	429:441	arg1	morphology					381:390	morphology	381:390	morphology	381:390	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	2	36	theme	nanocomposite	429:441	arg1	size					372:375	crystallite size	360:375	crystallite size	360:375	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	0	37	dep	delivery	65:72	arg1	applications					97:108	applications	97:108	applications	97:108	New core-shell hydroxyapatite/Gum-Acacia nanocomposites for drug delivery and tissue engineering applications.
30184795	2	38	theme	core-shell	411:420	arg1	nanocomposite					429:441	the synthesized core-shell HAP-GA nanocomposite	395:441	the synthesized core-shell HAP-GA nanocomposite	395:441	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	0	39	theme	tissue	78:83	arg1	engineering					85:95	tissue engineering	78:95	tissue engineering	78:95	New core-shell hydroxyapatite/Gum-Acacia nanocomposites for drug delivery and tissue engineering applications.
30184795	3	40	dep	10	691:692	arg1	to					688:689	to	688:689	to	688:689	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	3	41	theme	reaction	651:658	arg1	mixture					660:666	the reaction mixture	647:666	the reaction mixture	647:666	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	8	42	theme	1S	1331:1332	arg1	spectra					1334:1340	the O 1S spectra	1325:1340	the O 1S spectra	1325:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	9	43	theme	body	1508:1511	arg1	fluid					1513:1517	simulated body fluid	1498:1517	simulated body fluid	1498:1517	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	11	44	theme	hemolytic	1749:1757	arg1	studies					1759:1765	hemolytic studies	1749:1765	hemolytic studies	1749:1765	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	7	45	theme	photoelectron	1198:1210	arg1	spectroscopy					1212:1223	X-ray photoelectron spectroscopy	1192:1223	X-ray photoelectron spectroscopy	1192:1223	The elemental composition of the HAP samples was evaluated by X-ray photoelectron spectroscopy.
30184795	5	46	from	HAP	931:933	arg1	present					920:926	present	920:926	present	920:926	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	10	47	theme	HAP	1639:1641	arg1	core					1631:1634	the core	1627:1634	the core of HAP	1627:1641	The drug, naringenin, was loaded within the core of HAP by pellet pressing method.
30184795	4	48	theme	core	823:826	arg1	assembly					828:835	GA shell-HAP core assembly	810:835	GA shell-HAP core assembly	810:835	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	3	49	from	concentration	624:636	arg1	mixture					660:666	the reaction mixture	647:666	the reaction mixture	647:666	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	10	50	theme	pellet	1646:1651	arg1	method					1662:1667	pellet pressing method	1646:1667	pellet pressing method	1646:1667	The drug, naringenin, was loaded within the core of HAP by pellet pressing method.
30184795	2	51	theme	synthesized	399:409	arg1	nanocomposite					429:441	the synthesized core-shell HAP-GA nanocomposite	395:441	the synthesized core-shell HAP-GA nanocomposite	395:441	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	5	52	dep	spectroscopies	972:985	arg1	analyzed					1022:1029	analyzed	1022:1029	was analyzed by energy dispersive X-ray analysis	1018:1065	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	4	53	theme	GA	810:811	arg1	assembly					828:835	GA shell-HAP core assembly	810:835	GA shell-HAP core assembly	810:835	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	6	54	theme	HAP	1102:1104	arg1	's					1105:1106	the synthesized HAP's	1086:1106	the synthesized HAP's	1086:1106	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	3	55	theme	crystallite	532:542	arg1	size					544:547	The crystallite size	528:547	The crystallite size of GA-HAP nanocomposite	528:571	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	4	56	theme	10	865:866	arg1	%					867:867	%	867:867	%	867:867	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	4	57	theme	GA-HAP	869:874	arg1	composites					876:885	10% GA-HAP composites	865:885	10% GA-HAP composites	865:885	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	8	58	theme	GA	1380:1381	arg1	encapsulation					1363:1375	encapsulation	1363:1375	encapsulation of GA over the HAP particles	1363:1404	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	5	59	theme	energy	1034:1039	arg1	analysis					1058:1065	energy dispersive X-ray analysis	1034:1065	energy dispersive X-ray analysis	1034:1065	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	3	60	theme	nanocomposite	559:571	arg1	size					544:547	The crystallite size	528:547	The crystallite size of GA-HAP nanocomposite	528:571	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	11	61	theme	microbial	1730:1738	arg1	studies					1740:1746	microbial studies	1730:1746	microbial studies	1730:1746	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	1	62	theme	gum	145:147	arg1	nanocomposite					161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite	111:173	nanocomposite	161:173	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	7	63	theme	X-ray	1192:1196	arg1	spectroscopy					1212:1223	X-ray photoelectron spectroscopy	1192:1223	X-ray photoelectron spectroscopy	1192:1223	The elemental composition of the HAP samples was evaluated by X-ray photoelectron spectroscopy.
30184795	1	64	theme	NH4H2PO4as	306:315	arg1	precursors					317:326	Ca(NO3)2.4H2O and NH4H2PO4as precursors	288:326	Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P	288:339	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	11	65	theme	core-shell	1686:1695	arg1	composites					1701:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites	1670:1710	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	5	66	theme	functional	902:911	arg1	groups					913:918	functional groups	902:918	functional groups present in HAP	902:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	9	67	theme	Pellet	1459:1464	arg1	samples					1466:1472	Pellet samples	1459:1472	Pellet samples of HAP	1459:1479	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	5	68	theme	X-ray	1052:1056	arg1	analysis					1058:1065	energy dispersive X-ray analysis	1034:1065	energy dispersive X-ray analysis	1034:1065	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	5	69	theme	present	920:926	arg1	groups					913:918	functional groups	902:918	functional groups present in HAP	902:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	8	70	theme	nanocomposite	1444:1456	arg1	formation					1424:1432	the formation	1420:1432	the formation of GA-HAP nanocomposite	1420:1456	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	71	theme	1S	1280:1281	arg1	energies					1247:1254	binding energies 286.5 and 289.3 eV	1239:1273	energies	1247:1254	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	0	72	theme	core-shell	4:13	arg1	hydroxyapatite/Gum-Acacia					15:39	New core-shell hydroxyapatite/Gum-Acacia	0:39	New core-shell hydroxyapatite/Gum-Acacia	0:39	New core-shell hydroxyapatite/Gum-Acacia nanocomposites for drug delivery and tissue engineering applications.
30184795	1	73	theme	Core-shell	111:120	arg1	HAP					138:140	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite	111:173	HAP	138:140	Core-shell hydroxyapatite (HAP) - gum acacia (GA) nanocomposite, in which the HAP acts as a core while the GA serves as a shell, was synthesized by precipitation techniqueusing Ca(NO3)2.4H2O and NH4H2PO4as precursors for Ca and P, respectively.
30184795	8	74	from	energies	1247:1254	arg1	peaks					1291:1295	the peaks	1287:1295	the peaks at 530.6 eV and 532.1 eV of the O 1S spectra	1287:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	74	from	energies	1247:1254	arg1	peaks					1230:1234	The peaks	1226:1234	The peaks at binding energies 286.5 and 289.3 eV of C 1S	1226:1281	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	4	75	theme	electron	709:716	arg1	micrographs					718:728	Transmission electron micrographs	696:728	Transmission electron micrographs	696:728	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	2	76	theme	crystallite	360:370	arg1	size					372:375	crystallite size	360:375	crystallite size	360:375	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	0	77	theme	drug	60:63	arg1	delivery					65:72	drug delivery	60:72	drug delivery	60:72	New core-shell hydroxyapatite/Gum-Acacia nanocomposites for drug delivery and tissue engineering applications.
30184795	9	78	theme	simulated	1498:1506	arg1	fluid					1513:1517	simulated body fluid	1498:1517	simulated body fluid	1498:1517	Pellet samples of HAP were immersed in simulated body fluid to ascertain their bioactivity using scanning electron micrographs.
30184795	2	79	theme	HAP-GA	422:427	arg1	nanocomposite					429:441	the synthesized core-shell HAP-GA nanocomposite	395:441	the synthesized core-shell HAP-GA nanocomposite	395:441	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	5	80	theme	Raman	966:970	arg1	spectroscopies					972:985	FT-IR and Raman spectroscopies	956:985	spectroscopies	972:985	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	2	81	theme	electron	507:514	arg1	microscopy					516:525	transmission electron microscopy	494:525	transmission electron microscopy	494:525	The crystallite size and morphology of the synthesized core-shell HAP-GA nanocomposite was evaluated by X-ray diffraction measurement and transmission electron microscopy.
30184795	11	82	theme	MTT	1771:1773	arg1	assay					1775:1779	MTT assay	1771:1779	MTT assay	1771:1779	The drug-loaded core-shell HAP composites were subjected to microbial studies, hemolytic studies and MTT assay to assess their biocompatibility.
30184795	4	83	theme	HAP	767:769	arg1	particles					771:779	the HAP particles	763:779	the HAP particles	763:779	Transmission electron micrographs confirm encapsulation of GA over the HAP particles, leading to the formation of GA shell-HAP core assembly, which is quite evident for 10% GA-HAP composites.
30184795	6	84	theme	Ca/P	1072:1075	arg1	1.67					1124:1127	1.67	1124:1127	1.67	1124:1127	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	6	84	theme	Ca/P	1072:1075	arg1	ratio					1077:1081	The Ca/P ratio	1068:1081	The Ca/P ratio of the synthesized HAP's	1068:1106	The Ca/P ratio of the synthesized HAP's was found to be 1.67.
30184795	3	85	theme	GA	641:642	arg1	concentration					624:636	the concentration	620:636	the concentration of GA in the reaction mixture	620:666	The crystallite size of GA-HAP nanocomposite is markedly decreased from 89 nm to 63 nm when the concentration of GA in the reaction mixture is increased from 0 to 10%.
30184795	8	86	theme	O	1329:1329	arg1	spectra					1334:1340	the O 1S spectra	1325:1340	the O 1S spectra	1325:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	5	87	from	present	920:926	arg1	HAP					931:933	HAP	931:933	HAP	931:933	The nature of functional groups present in HAP was identified using FT-IR and Raman spectroscopies while its chemical composition was analyzed by energy dispersive X-ray analysis.
30184795	8	88	theme	spectra	1334:1340	arg1	532.1 eV					1313:1320	532.1 eV	1313:1320	532.1 eV	1313:1320	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	88	theme	spectra	1334:1340	arg1	530.6 eV					1300:1307	530.6 eV	1300:1307	530.6 eV	1300:1307	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	89	from	530.6 eV	1300:1307	arg1	peaks					1291:1295	the peaks	1287:1295	the peaks at 530.6 eV and 532.1 eV of the O 1S spectra	1287:1340	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30184795	8	89	from	530.6 eV	1300:1307	arg1	peaks					1230:1234	The peaks	1226:1234	The peaks at binding energies 286.5 and 289.3 eV of C 1S	1226:1281	The peaks at binding energies 286.5 and 289.3 eV of C 1S and the peaks at 530.6 eV and 532.1 eV of the O 1S spectra further substantiate encapsulation of GA over the HAP particles, resulting in the formation of GA-HAP nanocomposite.
30238304	9	0	dep	in	1713:1714	arg1	vivo					1716:1719	vivo	1716:1719	vivo	1716:1719	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	8	1	theme	Candida	1521:1527	arg1	culture					1529:1535	Candida culture	1521:1535	Candida culture	1521:1535	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	7	2	theme	halo	1160:1163	arg1	test					1170:1173	halo zone test	1160:1173	halo zone test	1160:1173	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	1	3	theme	Oropharyngeal	171:183	arg1	infection					217:225	a recurrent oral infection	200:225	a recurrent oral infection caused by Candida species	200:251	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	1	3	theme	Oropharyngeal	171:183	arg1	candidiasis					185:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	2	4	contain	containing	270:279	arg1	approach					319:326	the most common approach	303:326	the most common approach for treating oral candidiasis	303:356	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	2	4	contain	containing	270:279	arg1	formulation					258:268	Gel formulation	254:268	Gel formulation containing miconazole nitrate	254:298	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	2	4	contain	containing	270:279	arg2	nitrate					292:298	miconazole nitrate	281:298	miconazole nitrate	281:298	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	8	5	theme	miconazole	1542:1551	arg1	nitrate					1553:1559	miconazole nitrate	1542:1559	miconazole nitrate	1542:1559	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	7	6	theme	zone	1165:1168	arg1	test					1170:1173	halo zone test	1160:1173	halo zone test	1160:1173	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	6	7	theme	infrared	1120:1127	arg1	spectroscopy					1129:1140	infrared spectroscopy	1120:1140	infrared spectroscopy	1120:1140	The morphology of the systems was investigated by scanning electron microscopy; drug crystallinity was studied by X-ray, while interactions between polymers were analyzed by infrared spectroscopy.
30238304	4	8	theme	composited	588:597	arg1	systems					617:623	composited microparticulated systems	588:623	composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	588:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	3	9	theme	antifungal	393:402	arg1	therapies					404:412	traditional oral topical antifungal therapies	368:412	traditional oral topical antifungal therapies	368:412	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	7	10	dep	release	1204:1210	arg1	the					1200:1202	the	1200:1202	the	1200:1202	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	7	11	dep	albicans	1279:1286	arg1	cultures					1288:1295	cultures	1288:1295	Candida albicans cultures	1271:1295	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	9	12	theme	further	1676:1682	arg1	evaluation					1684:1693	further evaluation	1676:1693	further evaluation of its safety and in vivo efficacy	1676:1728	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	4	13	theme	nonionic	686:693	arg1	polymers					695:702	mucoadhesive cationic, anionic, and nonionic polymers	650:702	mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	650:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	6	14	theme	scanning	996:1003	arg1	microscopy					1014:1023	scanning electron microscopy	996:1023	scanning electron microscopy	996:1023	The morphology of the systems was investigated by scanning electron microscopy; drug crystallinity was studied by X-ray, while interactions between polymers were analyzed by infrared spectroscopy.
30238304	4	15	theme	fast	778:781	arg1	dilution					783:790	a fast dilution	776:790	a fast dilution of the drug by saliva	776:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	2	16	theme	common	312:317	arg1	approach					319:326	the most common approach	303:326	the most common approach for treating oral candidiasis	303:356	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	2	16	theme	common	312:317	arg1	formulation					258:268	Gel formulation	254:268	Gel formulation containing miconazole nitrate	254:298	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	4	17	theme	release	743:749	arg1	rate					751:754	the drug release rate	734:754	the drug release rate	734:754	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	3	18	theme	oral	380:383	arg1	therapies					404:412	traditional oral topical antifungal therapies	368:412	traditional oral topical antifungal therapies	368:412	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	9	19	theme	safety	1702:1707	arg1	evaluation					1684:1693	further evaluation	1676:1693	further evaluation of its safety and in vivo efficacy	1676:1728	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	8	20	theme	homogeneous	1399:1409	arg1	morphology					1411:1420	homogeneous morphology	1399:1420	homogeneous morphology (mean size of 1.7 ± 0.5 μm)	1399:1448	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	3	21	theme	oral	475:478	arg1	mucosa					480:485	the oral mucosa	471:485	the oral mucosa	471:485	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	3	22	theme	topical	385:391	arg1	therapies					404:412	traditional oral topical antifungal therapies	368:412	traditional oral topical antifungal therapies	368:412	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	8	23	theme	other	1569:1573	arg1	systems					1583:1589	the other assayed systems	1565:1589	the other assayed systems	1565:1589	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	0	24	theme	Mucoadhesive	42:53	arg1	Polymers					55:62	Natural Mucoadhesive Polymers	34:62	Natural Mucoadhesive Polymers with Promising Structural Properties	34:99	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	8	25	theme	assayed	1575:1581	arg1	systems					1583:1589	the other assayed systems	1565:1589	the other assayed systems	1565:1589	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	4	26	theme	microparticulated	599:615	arg1	systems					617:623	composited microparticulated systems	588:623	composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	588:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	8	27	dep	morphology	1411:1420	arg1	size					1428:1431	mean size	1423:1431	mean size of 1.7 ± 0.5 μm	1423:1447	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	9	28	theme	in	1713:1714	arg1	efficacy					1721:1728	in vivo efficacy	1713:1728	in vivo efficacy	1713:1728	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	3	29	contain	have	414:417	arg2	limitations					424:434	many limitations	419:434	many limitations	419:434	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	3	29	contain	have	414:417	arg1	therapies					404:412	traditional oral topical antifungal therapies	368:412	traditional oral topical antifungal therapies	368:412	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	1	30	theme	recurrent	202:210	arg1	infection					217:225	a recurrent oral infection	200:225	a recurrent oral infection caused by Candida species	200:251	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	1	30	theme	recurrent	202:210	arg1	candidiasis					185:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	0	31	theme	Composite	0:8	arg1	Microparticles					10:23	Composite Microparticles	0:23	Composite Microparticles	0:23	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	6	32	theme	electron	1005:1012	arg1	microscopy					1014:1023	scanning electron microscopy	996:1023	scanning electron microscopy	996:1023	The morphology of the systems was investigated by scanning electron microscopy; drug crystallinity was studied by X-ray, while interactions between polymers were analyzed by infrared spectroscopy.
30238304	2	33	theme	oral	341:344	arg1	candidiasis					346:356	oral candidiasis	341:356	oral candidiasis	341:356	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	1	34	theme	oral	212:215	arg1	infection					217:225	a recurrent oral infection	200:225	a recurrent oral infection caused by Candida species	200:251	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	1	34	theme	oral	212:215	arg1	candidiasis					185:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis	171:195	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	9	35	theme	oral	1805:1808	arg1	candidiasis					1810:1820	oral candidiasis	1805:1820	oral candidiasis	1805:1820	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	4	36	theme	work	566:569	arg1	aim					554:556	the aim	550:556	the aim of this work	550:569	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	9	37	theme	efficacy	1721:1728	arg1	evaluation					1684:1693	further evaluation	1676:1693	further evaluation of its safety and in vivo efficacy	1676:1728	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	8	38	theme	1.7 ± 0.5 μm	1436:1447	arg1	size					1428:1431	mean size	1423:1431	mean size of 1.7 ± 0.5 μm	1423:1447	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	3	39	theme	many	419:422	arg1	limitations					424:434	many limitations	419:434	many limitations	419:434	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	0	40	theme	Antifungal	128:137	arg1	Activity					139:146	the Antifungal Activity	124:146	the Antifungal Activity of Miconazole Nitrate	124:168	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	8	41	theme	drug	1478:1481	arg1	effect					1506:1511	better antifungal effect	1488:1511	better antifungal effect against Candida culture	1488:1535	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	41	theme	drug	1478:1481	arg1	effect					1464:1469	a protective effect	1451:1469	a protective effect of the drug	1451:1481	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	41	theme	drug	1478:1481	arg1	morphology					1411:1420	homogeneous morphology	1399:1420	homogeneous morphology (mean size of 1.7 ± 0.5 μm)	1399:1448	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	42	theme	mean	1423:1426	arg1	size					1428:1431	mean size	1423:1431	mean size of 1.7 ± 0.5 μm	1423:1447	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	4	43	theme	cationic	663:670	arg1	polymers					695:702	mucoadhesive cationic, anionic, and nonionic polymers	650:702	mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	650:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	3	44	theme	contact	453:459	arg1	time					461:464	short contact time	447:464	short contact time	447:464	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	0	45	theme	Miconazole	151:160	arg1	Nitrate					162:168	Miconazole Nitrate	151:168	Miconazole Nitrate	151:168	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	8	46	theme	microparticulated	1319:1335	arg1	system					1337:1342	The most appropriate microparticulated system	1298:1342	The most appropriate microparticulated system	1298:1342	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	46	theme	microparticulated	1319:1335	arg1	one					1352:1354	one	1352:1354	one	1352:1354	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	5	47	dep	chitosan	892:899	arg1	methylcellulose					929:943	methylcellulose	929:943	methylcellulose	929:943	Microparticulated systems were prepared by the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose.
30238304	4	48	theme	polymers	695:702	arg1	combinations					634:645	combinations	634:645	combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	634:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	5	49	theme	spray	862:866	arg1	method					875:880	the spray drying method	858:880	the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose	858:943	Microparticulated systems were prepared by the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose.
30238304	0	50	theme	Natural	34:40	arg1	Polymers					55:62	Natural Mucoadhesive Polymers	34:62	Natural Mucoadhesive Polymers with Promising Structural Properties	34:99	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	6	51	theme	drug	1026:1029	arg1	crystallinity					1031:1043	drug crystallinity	1026:1043	drug crystallinity	1026:1043	The morphology of the systems was investigated by scanning electron microscopy; drug crystallinity was studied by X-ray, while interactions between polymers were analyzed by infrared spectroscopy.
30238304	2	52	theme	Gel	254:256	arg1	approach					319:326	the most common approach	303:326	the most common approach for treating oral candidiasis	303:356	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	2	52	theme	Gel	254:256	arg1	formulation					258:268	Gel formulation	254:268	Gel formulation containing miconazole nitrate	254:298	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	5	53	theme	drying	868:873	arg1	method					875:880	the spray drying method	858:880	the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose	858:943	Microparticulated systems were prepared by the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose.
30238304	1	54	theme	Candida	237:243	arg1	species					245:251	Candida species	237:251	Candida species	237:251	Oropharyngeal candidiasis is a recurrent oral infection caused by Candida species.
30238304	6	55	theme	systems	968:974	arg1	morphology					950:959	The morphology	946:959	The morphology of the systems	946:974	The morphology of the systems was investigated by scanning electron microscopy; drug crystallinity was studied by X-ray, while interactions between polymers were analyzed by infrared spectroscopy.
30238304	3	56	theme	traditional	368:378	arg1	therapies					404:412	traditional oral topical antifungal therapies	368:412	traditional oral topical antifungal therapies	368:412	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	4	57	theme	drug	738:741	arg1	rate					751:754	the drug release rate	734:754	the drug release rate	734:754	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	3	58	theme	short	447:451	arg1	time					461:464	short contact time	447:464	short contact time	447:464	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	0	59	theme	Nitrate	162:168	arg1	Activity					139:146	the Antifungal Activity	124:146	the Antifungal Activity of Miconazole Nitrate	124:168	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	8	60	theme	antifungal	1495:1504	arg1	effect					1506:1511	better antifungal effect	1488:1511	better antifungal effect against Candida culture	1488:1535	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	7	61	theme	Drug	1143:1146	arg1	release					1148:1154	Drug release	1143:1154	Drug release	1143:1154	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	9	62	theme	alternative	1753:1763	arg1	approach					1632:1639	this approach	1627:1639	this approach	1627:1639	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	9	62	theme	alternative	1753:1763	arg1	system					1777:1782	an alternative therapeutic system	1750:1782	an alternative therapeutic system for the treatment of oral candidiasis	1750:1820	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	8	63	theme	better	1488:1493	arg1	effect					1506:1511	better antifungal effect	1488:1511	better antifungal effect against Candida culture	1488:1535	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	64	theme	protective	1453:1462	arg1	effect					1464:1469	a protective effect	1451:1469	a protective effect of the drug	1451:1481	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	9	65	theme	candidiasis	1810:1820	arg1	treatment					1792:1800	the treatment	1788:1800	the treatment of oral candidiasis	1788:1820	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	8	66	theme	appropriate	1307:1317	arg1	system					1337:1342	The most appropriate microparticulated system	1298:1342	The most appropriate microparticulated system	1298:1342	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	8	66	theme	appropriate	1307:1317	arg1	one					1352:1354	one	1352:1354	one	1352:1354	The most appropriate microparticulated system was the one based on chitosan and gelatin which showed homogeneous morphology (mean size of 1.7 ± 0.5 μm), a protective effect of the drug, and better antifungal effect against Candida culture than miconazole nitrate and the other assayed systems.
30238304	9	67	theme	therapeutic	1765:1775	arg1	approach					1632:1639	this approach	1627:1639	this approach	1627:1639	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	9	67	theme	therapeutic	1765:1775	arg1	system					1777:1782	an alternative therapeutic system	1750:1782	an alternative therapeutic system for the treatment of oral candidiasis	1750:1820	Taking into account these results, this approach should be seriously considered for further evaluation of its safety and in vivo efficacy to be considered as an alternative therapeutic system for the treatment of oral candidiasis.
30238304	4	68	theme	drug	799:802	arg1	dilution					783:790	a fast dilution	776:790	a fast dilution of the drug by saliva	776:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	3	69	theme	various	521:527	arg1	doses					529:533	various doses	521:533	various doses per day	521:541	However, traditional oral topical antifungal therapies have many limitations, including short contact time with the oral mucosa and the necessity to administrate various doses per day.
30238304	0	70	theme	Structural	79:88	arg1	Properties					90:99	Promising Structural Properties	69:99	Promising Structural Properties	69:99	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	4	71	theme	anionic	673:679	arg1	polymers					695:702	mucoadhesive cationic, anionic, and nonionic polymers	650:702	mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	650:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	2	72	theme	miconazole	281:290	arg1	nitrate					292:298	miconazole nitrate	281:298	miconazole nitrate	281:298	Gel formulation containing miconazole nitrate is the most common approach for treating oral candidiasis.
30238304	0	73	with	Polymers	55:62	arg1	Properties					90:99	Promising Structural Properties	69:99	Promising Structural Properties	69:99	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	4	74	theme	mucoadhesive	650:661	arg1	polymers					695:702	mucoadhesive cationic, anionic, and nonionic polymers	650:702	mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva	650:812	Thus, the aim of this work was to formulate composited microparticulated systems based on combinations of mucoadhesive cationic, anionic, and nonionic polymers that could protect and modify the drug release rate and therefore avoid a fast dilution of the drug by saliva.
30238304	0	75	theme	Promising	69:77	arg1	Properties					90:99	Promising Structural Properties	69:99	Promising Structural Properties	69:99	Composite Microparticles Based on Natural Mucoadhesive Polymers with Promising Structural Properties to Protect and Improve the Antifungal Activity of Miconazole Nitrate.
30238304	5	76	theme	Microparticulated	815:831	arg1	systems					833:839	Microparticulated systems	815:839	Microparticulated systems	815:839	Microparticulated systems were prepared by the spray drying method employing chitosan, gelatin, and hydroxypropyl methylcellulose.
30238304	7	77	theme	Candida	1271:1277	arg1	albicans					1279:1286	Candida albicans cultures	1271:1295	Candida albicans cultures	1271:1295	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	7	78	theme	systems	1232:1238	arg1	activity					1216:1223	activity	1216:1223	activity	1216:1223	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
30238304	7	78	theme	systems	1232:1238	arg1	release					1204:1210	release	1204:1210	release	1204:1210	Drug release and halo zone test were employed to analyze the release and activity of the systems loaded with miconazole against Candida albicans cultures.
31914668	6	0	theme	fatty	801:805	arg1	SCFAs					814:818	SCFAs	814:818	SCFAs	814:818	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	6	0	theme	fatty	801:805	arg1	acids					807:811	short-chain fatty acids	789:811	short-chain fatty acids (SCFAs)	789:819	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	8	1	theme	diabetic	1215:1222	arg1	patients					1224:1231	diabetic patients	1215:1231	diabetic patients	1215:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	0	2	theme	microbiota	87:96	arg1	composition					98:108	gut microbiota composition	83:108	gut microbiota composition	83:108	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	6	3	theme	treatment	753:761	arg1	weeks					744:748	8 weeks	742:748	8 weeks of treatment	742:761	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	7	4	theme	intestinal	889:898	arg1	function					908:915	intestinal barrier function	889:915	intestinal barrier function	889:915	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	2	5	theme	regulatory	355:364	arg1	effect					366:371	the regulatory effect	351:371	the regulatory effect of PLPE on gut microbiota	351:397	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	6	6	theme	short-chain	789:799	arg1	SCFAs					814:818	SCFAs	814:818	SCFAs	814:818	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	6	6	theme	short-chain	789:799	arg1	acids					807:811	short-chain fatty acids	789:811	short-chain fatty acids (SCFAs)	789:819	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	7	7	theme	barrier	900:906	arg1	function					908:915	intestinal barrier function	889:915	intestinal barrier function	889:915	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	3	8	theme	Sprague-Dawley	523:536	arg1	rats					538:541	24 male Sprague-Dawley rats	515:541	24 male Sprague-Dawley rats	515:541	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	1	9	theme	gut	279:281	arg1	microbiota					283:292	gut microbiota	279:292	gut microbiota	279:292	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	2	10	theme	molecular	408:416	arg1	mechanism					418:426	the molecular mechanism	404:426	the molecular mechanism underlying improvement of insulin resistance	404:471	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	8	11	theme	potential	1133:1141	arg1	application					1143:1153	potential application	1133:1153	potential application	1133:1153	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	11	theme	potential	1133:1141	arg1	prebiotic					1160:1168	a prebiotic	1158:1168	a prebiotic for regulating gut microbiota composition in diabetic patients	1158:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	7	12	from	function	908:915	arg1	blood					960:964	blood	960:964	blood	960:964	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	8	13	theme	major	1112:1116	arg1	polysaccharides					1088:1102	polysaccharides	1088:1102	polysaccharides	1088:1102	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	13	theme	major	1112:1116	arg1	component					1118:1126	the major component	1108:1126	the major component	1108:1126	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	6	14	theme	acids	807:811	arg1	levels					779:784	levels	779:784	levels of short-chain fatty acids (SCFAs)	779:819	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	2	15	from	effect	366:371	arg1	microbiota					388:397	gut microbiota	384:397	gut microbiota	384:397	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	3	16	theme	male	518:521	arg1	rats					538:541	24 male Sprague-Dawley rats	515:541	24 male Sprague-Dawley rats	515:541	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	0	17	theme	linteus	10:16	arg1	extract					33:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	2	18	theme	type	482:485	arg1	model					502:506	a type 2 diabetic rat model	480:506	a type 2 diabetic rat model	480:506	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	7	19	theme	insulin	1027:1033	arg1	resistance					1035:1044	insulin resistance	1027:1044	insulin resistance	1027:1044	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	0	20	theme	gut	83:85	arg1	composition					98:108	gut microbiota composition	83:108	gut microbiota composition	83:108	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	2	21	theme	gut	384:386	arg1	microbiota					388:397	gut microbiota	384:397	gut microbiota	384:397	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	0	22	theme	Phellinus	0:8	arg1	extract					33:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	7	23	from	content	949:955	arg1	blood					960:964	blood	960:964	blood	960:964	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	3	24	dep	metformin	662:670	arg1	mg/kg					677:681	120 mg/kg	673:681	metformin (120 mg/kg	662:681	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	1	25	theme	hypoglycemic	115:126	arg1	effect					128:133	The hypoglycemic effect	111:133	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE)	111:184	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	26	theme	several	209:215	arg1	studies					226:232	several previous studies	209:232	several previous studies	209:232	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	0	27	theme	polysaccharide	18:31	arg1	extract					33:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract	0:39	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	5	28	dep	bw	708:709	arg1	administration					720:733	oral administration	715:733	oral administration	715:733	bw) by oral administration.
31914668	3	29	theme	normal	629:634	arg1	saline					621:626	saline	621:626	saline (normal and model control group)	621:659	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	3	29	theme	normal	629:634	arg1	group					654:658	normal and model control group	629:658	normal and model control group	629:658	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	1	30	theme	previous	217:224	arg1	studies					226:232	several previous studies	209:232	several previous studies	209:232	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	0	31	theme	insulin	50:56	arg1	resistance					58:67	insulin resistance	50:67	insulin resistance	50:67	Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition.
31914668	4	32	dep	PLPE	692:695	arg1	mg/kg					702:706	600 mg/kg	698:706	PLPE (600 mg/kg	692:706	bw), and PLPE (600 mg/kg.
31914668	7	33	theme	systemic	993:1000	arg1	inflammation					1002:1013	systemic inflammation	993:1013	systemic inflammation	993:1013	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	8	34	theme	microbiota	1189:1198	arg1	composition					1200:1210	gut microbiota composition	1185:1210	gut microbiota composition in diabetic patients	1185:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	35	theme	gut	1185:1187	arg1	composition					1200:1210	gut microbiota composition	1185:1210	gut microbiota composition in diabetic patients	1185:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	1	36	theme	hypoglycemic	303:314	arg1	effect					316:321	the hypoglycemic effect	299:321	the hypoglycemic effect	299:321	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	3	37	theme	saline	621:626	arg1	intervention					605:616	intervention	605:616	intervention of saline (normal and model control group), metformin (120 mg/kg	605:681	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	8	38	from	composition	1200:1210	arg1	patients					1224:1231	diabetic patients	1215:1231	diabetic patients	1215:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	5	39	theme	oral	715:718	arg1	administration					720:733	oral administration	715:733	oral administration	715:733	bw) by oral administration.
31914668	6	40	theme	bacteria	862:869	arg1	abundance					834:842	abundance	834:842	abundance of SCFA-producing bacteria	834:869	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	2	41	theme	resistance	462:471	arg1	improvement					439:449	improvement	439:449	improvement of insulin resistance	439:471	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	6	42	theme	SCFA-producing	847:860	arg1	bacteria					862:869	SCFA-producing bacteria	847:869	SCFA-producing bacteria	847:869	After 8 weeks of treatment, PLPE increased levels of short-chain fatty acids (SCFAs) by enhancing abundance of SCFA-producing bacteria.
31914668	8	43	from	component	1118:1126	arg1	which					1082:1086	which	1082:1086	which	1082:1086	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	1	44	theme	Phellinus	138:146	arg1	PLPE					180:183	PLPE	180:183	PLPE	180:183	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	44	theme	Phellinus	138:146	arg1	extract					171:177	Phellinus linteus polysaccharide extract	138:177	Phellinus linteus polysaccharide extract (PLPE)	138:184	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	45	theme	extract	171:177	arg1	effect					128:133	The hypoglycemic effect	111:133	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE)	111:184	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	46	theme	linteus	148:154	arg1	PLPE					180:183	PLPE	180:183	PLPE	180:183	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	46	theme	linteus	148:154	arg1	extract					171:177	Phellinus linteus polysaccharide extract	138:177	Phellinus linteus polysaccharide extract (PLPE)	138:184	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	8	47	dep	PLPE	1073:1076	arg1	polysaccharides					1088:1102	polysaccharides	1088:1102	polysaccharides	1088:1102	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	47	dep	PLPE	1073:1076	arg1	component					1118:1126	the major component	1108:1126	the major component	1108:1126	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	3	48	theme	model	640:644	arg1	saline					621:626	saline	621:626	saline (normal and model control group)	621:659	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	3	48	theme	model	640:644	arg1	group					654:658	normal and model control group	629:658	normal and model control group	629:658	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	7	49	theme	reduced	921:927	arg1	content					949:955	reduced lipopolysaccharides content	921:955	reduced lipopolysaccharides content in blood	921:964	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	1	50	theme	polysaccharide	156:169	arg1	PLPE					180:183	PLPE	180:183	PLPE	180:183	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	1	50	theme	polysaccharide	156:169	arg1	extract					171:177	Phellinus linteus polysaccharide extract	138:177	Phellinus linteus polysaccharide extract (PLPE)	138:184	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	2	51	theme	rat	498:500	arg1	model					502:506	a type 2 diabetic rat model	480:506	a type 2 diabetic rat model	480:506	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	3	52	theme	control	646:652	arg1	saline					621:626	saline	621:626	saline (normal and model control group)	621:659	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	3	52	theme	control	646:652	arg1	group					654:658	normal and model control group	629:658	normal and model control group	629:658	Here, 24 male Sprague-Dawley rats were randomly divided into four groups that were subjected to intervention of saline (normal and model control group), metformin (120 mg/kg.
31914668	7	53	theme	lipopolysaccharides	929:947	arg1	content					949:955	reduced lipopolysaccharides content	921:955	reduced lipopolysaccharides content in blood	921:964	SCFAs maintained intestinal barrier function and reduced lipopolysaccharides content in blood, thereby helping to reduce systemic inflammation and reverse insulin resistance.
31914668	2	54	theme	PLPE	376:379	arg1	effect					366:371	the regulatory effect	351:371	the regulatory effect of PLPE on gut microbiota	351:397	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	1	55	theme	functional	243:252	arg1	interactions					254:265	the functional interactions	239:265	the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect	239:321	The hypoglycemic effect of Phellinus linteus polysaccharide extract (PLPE) has been documented in several previous studies, but the functional interactions among PLPE, gut microbiota, and the hypoglycemic effect remain unclear.
31914668	2	56	theme	diabetic	489:496	arg1	model					502:506	a type 2 diabetic rat model	480:506	a type 2 diabetic rat model	480:506	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
31914668	8	57	contain	has	1129:1131	arg2	prebiotic					1160:1168	a prebiotic	1158:1168	a prebiotic for regulating gut microbiota composition in diabetic patients	1158:1231	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	57	contain	has	1129:1131	arg2	application					1143:1153	potential application	1133:1153	potential application	1133:1153	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	8	57	contain	has	1129:1131	arg1	PLPE					1073:1076	PLPE	1073:1076	PLPE (in which polysaccharides are the major component)	1073:1127	Our findings suggest that PLPE (in which polysaccharides are the major component) has potential application as a prebiotic for regulating gut microbiota composition in diabetic patients.
31914668	2	58	theme	insulin	454:460	arg1	resistance					462:471	insulin resistance	454:471	insulin resistance	454:471	We examined the regulatory effect of PLPE on gut microbiota, and the molecular mechanism underlying improvement of insulin resistance, using a type 2 diabetic rat model.
30641263	0	0	theme	bone	89:92	arg1	model					101:105	a sheep bone defect model	81:105	a sheep bone defect model	81:105	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	3	1	link	naturally-derived	585:601	arg1	hyaluronate					626:636	hyaluronate	626:636	hyaluronate	626:636	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	1	link	naturally-derived	585:601	arg1	alginate					613:620	alginate	613:620	alginate	613:620	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	1	link	naturally-derived	585:601	arg1	polymers					603:610	two naturally-derived polymers	581:610	two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres	581:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	8	2	theme	healing	1650:1656	arg1	therapies					1632:1640	cell-based therapies	1621:1640	cell-based therapies of bone healing	1621:1656	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	7	3	theme	osteoid	1346:1352	arg1	formation					1354:1362	osteoid formation	1346:1362	osteoid formation	1346:1362	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	6	4	theme	autologous	1096:1105	arg1	MSCs					1125:1128	autologous, culture-expanded MSCs	1096:1128	autologous, culture-expanded MSCs	1096:1128	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	0	5	theme	sheep	83:87	arg1	model					101:105	a sheep bone defect model	81:105	a sheep bone defect model	81:105	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	6	6	dep	autologous	1096:1105	arg1	culture-expanded					1108:1123	culture-expanded	1108:1123	culture-expanded	1108:1123	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	3	7	dep	polymers	603:610	arg1	hyaluronate					626:636	hyaluronate	626:636	hyaluronate	626:636	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	7	dep	polymers	603:610	arg1	alginate					613:620	alginate	613:620	alginate	613:620	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	7	dep	polymers	603:610	arg1	polymers					603:610	two naturally-derived polymers	581:610	two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres	581:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	8	8	theme	cell-based	1621:1630	arg1	therapies					1632:1640	cell-based therapies	1621:1640	cell-based therapies of bone healing	1621:1656	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	1	9	theme	osteogenic	253:262	arg1	differentiation					264:278	osteogenic differentiation	253:278	osteogenic differentiation	253:278	The efficacy of cell-based therapies as an alternative to autologous bone grafts requires biomaterials to localize cells at the defect and drive osteogenic differentiation.
30641263	5	10	theme	adhesive	785:792	arg1	RGD					837:839	RGD	837:839	RGD	837:839	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	10	theme	adhesive	785:792	arg1	Acid					831:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid	781:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	781:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	11	from	polymers	852:859	arg1	presentation					765:776	The presentation	761:776	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	761:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	11	from	polymers	852:859	arg1	Acid					831:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid	781:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	781:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	11	from	polymers	852:859	arg1	RGD					837:839	RGD	837:839	RGD	837:839	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	2	12	theme	cell	301:304	arg1	vehicles					315:322	ideal cell delivery vehicles	295:322	ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties	295:405	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	2	12	theme	cell	301:304	arg1	Hydrogels					281:289	Hydrogels	281:289	Hydrogels	281:289	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	0	13	theme	defect	94:99	arg1	model					101:105	a sheep bone defect model	81:105	a sheep bone defect model	81:105	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	8	14	theme	bone	1645:1648	arg1	healing					1650:1656	bone healing	1645:1656	bone healing	1645:1656	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	7	15	theme	weeks	1207:1211	arg1	post-implantation					1213:1229	12 weeks post-implantation	1204:1229	12 weeks post-implantation	1204:1229	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	2	16	theme	ideal	295:299	arg1	vehicles					315:322	ideal cell delivery vehicles	295:322	ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties	295:405	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	2	16	theme	ideal	295:299	arg1	Hydrogels					281:289	Hydrogels	281:289	Hydrogels	281:289	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	3	17	theme	mesenchymal	515:525	arg1	MSCs					542:545	MSCs	542:545	MSCs	542:545	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	17	theme	mesenchymal	515:525	arg1	cells					535:539	mesenchymal stromal cells	515:539	mesenchymal stromal cells (MSCs)	515:546	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	5	18	theme	Arginine-Glycine-Aspartic	805:829	arg1	RGD					837:839	RGD	837:839	RGD	837:839	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	18	theme	Arginine-Glycine-Aspartic	805:829	arg1	Acid					831:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid	781:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	781:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	7	19	theme	untreated	1414:1422	arg1	defects					1424:1430	untreated defects	1414:1430	untreated defects	1414:1430	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	5	20	from	presentation	765:776	arg1	polymers					852:859	both polymers	847:859	both polymers	847:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	1	21	theme	autologous	166:175	arg1	grafts					182:187	autologous bone grafts	166:187	autologous bone grafts	166:187	The efficacy of cell-based therapies as an alternative to autologous bone grafts requires biomaterials to localize cells at the defect and drive osteogenic differentiation.
30641263	5	22	theme	Acid	831:834	arg1	presentation					765:776	The presentation	761:776	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	761:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	3	23	theme	composite	553:561	arg1	hydrogel					563:570	a composite hydrogel	551:570	a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres	551:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	6	24	theme	bone	1188:1191	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	1	25	theme	bone	177:180	arg1	grafts					182:187	autologous bone grafts	166:187	autologous bone grafts	166:187	The efficacy of cell-based therapies as an alternative to autologous bone grafts requires biomaterials to localize cells at the defect and drive osteogenic differentiation.
30641263	0	26	theme	mineralized	11:21	arg1	composite					42:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	7	27	theme	vessel	1330:1335	arg1	density					1337:1343	blood vessel density	1324:1343	blood vessel density	1324:1343	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	3	28	theme	naturally-derived	585:601	arg1	hyaluronate					626:636	hyaluronate	626:636	hyaluronate	626:636	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	28	theme	naturally-derived	585:601	arg1	alginate					613:620	alginate	613:620	alginate	613:620	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	28	theme	naturally-derived	585:601	arg1	polymers					603:610	two naturally-derived polymers	581:610	two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres	581:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	7	29	theme	composite	1274:1282	arg1	gels					1284:1287	composite gels	1274:1287	composite gels	1274:1287	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	6	30	theme	ovine	1170:1174	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	0	31	theme	Injectable	0:9	arg1	composite					42:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	6	32	theme	bone	1064:1067	arg1	healing					1069:1075	bone healing	1064:1075	bone healing	1064:1075	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	8	33	theme	osteoconductive	1476:1490	arg1	hydrogels					1492:1500	osteoconductive hydrogels	1476:1500	osteoconductive hydrogels	1476:1500	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	4	34	theme	hydrogels	718:726	arg1	properties					700:709	Mechanical properties	689:709	Mechanical properties of the hydrogels	689:726	Mechanical properties of the hydrogels were dependent upon composition.
30641263	5	35	theme	MSCs	913:916	arg1	differentiation					888:902	greater osteogenic differentiation	869:902	greater osteogenic differentiation of ovine MSCs	869:916	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	0	36	theme	microsphere-loaded	23:40	arg1	composite					42:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite	0:50	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	8	37	with	formation	1518:1526	arg1	MSCs					1544:1547	autologous MSCs	1533:1547	autologous MSCs	1533:1547	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	8	38	theme	bone	1567:1570	arg1	model					1579:1583	a large animal bone defect model	1552:1583	a large animal bone defect model	1552:1583	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	2	39	theme	delivery	306:313	arg1	vehicles					315:322	ideal cell delivery vehicles	295:322	ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties	295:405	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	2	39	theme	delivery	306:313	arg1	Hydrogels					281:289	Hydrogels	281:289	Hydrogels	281:289	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	8	40	theme	hydrogels	1492:1500	arg1	capacity					1464:1471	the capacity	1460:1471	the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model	1460:1583	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	6	41	theme	induced	1146:1152	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	6	42	theme	construct	1041:1049	arg1	capacity					1024:1031	the capacity	1020:1031	the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect	1020:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	7	43	theme	acellular	1396:1404	arg1	gels					1406:1409	acellular gels	1396:1409	acellular gels	1396:1409	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	6	44	theme	surgical	1137:1144	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	6	45	theme	crest	1182:1186	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	7	46	from	increases	1311:1319	arg1	formation					1374:1382	bone formation	1369:1382	bone formation	1369:1382	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	7	46	from	increases	1311:1319	arg1	density					1337:1343	blood vessel density	1324:1343	blood vessel density	1324:1343	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	7	46	from	increases	1311:1319	arg1	formation					1354:1362	osteoid formation	1346:1362	osteoid formation	1346:1362	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	8	47	theme	bone	1513:1516	arg1	formation					1518:1526	bone formation	1513:1526	bone formation with autologous MSCs	1513:1547	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	2	48	theme	osteoconductive	425:439	arg1	components					441:450	osteoconductive components	425:450	osteoconductive components that nucleate mineral	425:472	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	5	49	theme	greater	869:875	arg1	differentiation					888:902	greater osteogenic differentiation	869:902	greater osteogenic differentiation of ovine MSCs	869:916	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	8	50	theme	defect	1572:1577	arg1	model					1579:1583	a large animal bone defect model	1552:1583	a large animal bone defect model	1552:1583	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	8	51	theme	autologous	1533:1542	arg1	MSCs					1544:1547	autologous MSCs	1533:1547	autologous MSCs	1533:1547	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	3	52	theme	stromal	527:533	arg1	MSCs					542:545	MSCs	542:545	MSCs	542:545	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	52	theme	stromal	527:533	arg1	cells					535:539	mesenchymal stromal cells	515:539	mesenchymal stromal cells (MSCs)	515:546	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	0	53	theme	bone	66:69	arg1	repair					71:76	bone repair	66:76	bone repair	66:76	Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model.
30641263	7	54	theme	significant	1299:1309	arg1	increases					1311:1319	significant increases	1299:1319	significant increases in blood vessel density, osteoid formation, and bone formation	1299:1382	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	8	55	theme	animal	1560:1565	arg1	model					1579:1583	a large animal bone defect model	1552:1583	a large animal bone defect model	1552:1583	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	3	56	theme	biomineralized	650:663	arg1	microspheres					675:686	biomineralized polymeric microspheres	650:686	biomineralized polymeric microspheres	650:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	1	57	theme	cell-based	124:133	arg1	therapies					135:143	cell-based therapies	124:143	cell-based therapies as an alternative to autologous bone grafts	124:187	The efficacy of cell-based therapies as an alternative to autologous bone grafts requires biomaterials to localize cells at the defect and drive osteogenic differentiation.
30641263	3	58	contain	containing	639:648	arg2	microspheres					675:686	biomineralized polymeric microspheres	650:686	biomineralized polymeric microspheres	650:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	58	contain	containing	639:648	arg1	hyaluronate					626:636	hyaluronate	626:636	hyaluronate	626:636	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	58	contain	containing	639:648	arg1	alginate					613:620	alginate	613:620	alginate	613:620	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	3	58	contain	containing	639:648	arg1	polymers					603:610	two naturally-derived polymers	581:610	two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres	581:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	6	59	dep	induced	1146:1152	arg1	critical-sized					1155:1168	critical-sized	1155:1168	critical-sized	1155:1168	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	8	60	theme	promising	1599:1607	arg1	vehicle					1609:1615	a promising vehicle	1597:1615	a promising vehicle for cell-based therapies of bone healing	1597:1656	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	3	61	theme	polymeric	665:673	arg1	microspheres					675:686	biomineralized polymeric microspheres	650:686	biomineralized polymeric microspheres	650:686	To address this challenge, we entrapped mesenchymal stromal cells (MSCs) in a composite hydrogel based on two naturally-derived polymers (alginate and hyaluronate) containing biomineralized polymeric microspheres.
30641263	7	62	theme	blood	1324:1328	arg1	density					1337:1343	blood vessel density	1324:1343	blood vessel density	1324:1343	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	1	63	theme	therapies	135:143	arg1	efficacy					112:119	The efficacy	108:119	The efficacy of cell-based therapies as an alternative to autologous bone grafts	108:187	The efficacy of cell-based therapies as an alternative to autologous bone grafts requires biomaterials to localize cells at the defect and drive osteogenic differentiation.
30641263	2	64	theme	instructional	341:353	arg1	cues					355:358	instructional cues	341:358	instructional cues	341:358	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	4	65	theme	Mechanical	689:698	arg1	properties					700:709	Mechanical properties	689:709	Mechanical properties of the hydrogels	689:726	Mechanical properties of the hydrogels were dependent upon composition.
30641263	8	66	theme	large	1554:1558	arg1	model					1579:1583	a large animal bone defect model	1552:1583	a large animal bone defect model	1552:1583	These findings demonstrate the capacity of osteoconductive hydrogels to promote bone formation with autologous MSCs in a large animal bone defect model and provide a promising vehicle for cell-based therapies of bone healing.
30641263	2	67	theme	mechanical	385:394	arg1	properties					396:405	mechanical properties	385:405	mechanical properties	385:405	Hydrogels are ideal cell delivery vehicles that can provide instructional cues via their composition or mechanical properties but commonly lack osteoconductive components that nucleate mineral.
30641263	6	68	theme	iliac	1176:1180	arg1	defect					1193:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	a surgical induced, critical-sized ovine iliac crest bone defect	1135:1198	We then evaluated the capacity of this construct to stimulate bone healing when transplanting autologous, culture-expanded MSCs into a surgical induced, critical-sized ovine iliac crest bone defect.
30641263	5	69	theme	ovine	907:911	arg1	MSCs					913:916	ovine MSCs	907:916	ovine MSCs	907:916	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	7	70	theme	bone	1369:1372	arg1	formation					1374:1382	bone formation	1369:1382	bone formation	1369:1382	At 12 weeks post-implantation, defects treated with MSCs transplanted in composite gels exhibited significant increases in blood vessel density, osteoid formation, and bone formation compared to acellular gels or untreated defects.
30641263	5	71	theme	osteogenic	877:886	arg1	differentiation					888:902	greater osteogenic differentiation	869:902	greater osteogenic differentiation of ovine MSCs	869:916	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	72	theme	tripeptide	794:803	arg1	RGD					837:839	RGD	837:839	RGD	837:839	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30641263	5	72	theme	tripeptide	794:803	arg1	Acid					831:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid	781:834	the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers	781:859	The presentation of the adhesive tripeptide Arginine-Glycine-Aspartic Acid (RGD) from both polymers induced greater osteogenic differentiation of ovine MSCs in vitro compared to gels formed of RGD-alginate or RGD-alginate/hyaluronate alone.
30304700	1	0	from	substances	196:205	arg1	biofilms					216:223	biofilms	216:223	biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	216:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	1	theme	main	167:170	arg1	EPS					208:210	EPS	208:210	EPS	208:210	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	1	theme	main	167:170	arg1	substances					196:205	the main extracellular polymeric substances	163:205	the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	163:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	8	2	theme	potential	1489:1497	arg1	target					1514:1519	a potential macromolecular target	1487:1519	a potential macromolecular target to improve anti-infectious therapies	1487:1556	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	0	3	theme	physico-chemical	72:87	arg1	properties					89:98	the physico-chemical properties	68:98	the physico-chemical properties of alginate-containing biofilms	68:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	1	4	theme	extracellular	172:184	arg1	EPS					208:210	EPS	208:210	EPS	208:210	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	4	theme	extracellular	172:184	arg1	substances					196:205	the main extracellular polymeric substances	163:205	the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	163:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	5	theme	patients	249:256	arg1	biofilms					216:223	biofilms	216:223	biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	216:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	0	6	theme	alginate-containing	103:121	arg1	biofilms					123:130	alginate-containing biofilms	103:130	alginate-containing biofilms	103:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	0	7	from	Interaction	0:10	arg1	properties					89:98	the physico-chemical properties	68:98	the physico-chemical properties of alginate-containing biofilms	68:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	7	8	theme	ratio	1331:1335	arg1	composition					1318:1328	the polysaccharide motif composition	1293:1328	the polysaccharide motif composition (ratio M/G)	1293:1340	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	7	8	theme	ratio	1331:1335	arg1	M/G					1337:1339	ratio M/G	1331:1339	ratio M/G	1331:1339	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	2	9	theme	Gentamicin	295:304	arg1	GS					315:316	GS	315:316	GS	315:316	Gentamicin sulfate (GS) can strongly bind to alginate resulting in loss of pharmacological activity; however neither the mechanism nor its repercussion is fully understood.
30304700	2	9	theme	Gentamicin	295:304	arg1	sulfate					306:312	Gentamicin sulfate	295:312	Gentamicin sulfate (GS)	295:317	Gentamicin sulfate (GS) can strongly bind to alginate resulting in loss of pharmacological activity; however neither the mechanism nor its repercussion is fully understood.
30304700	5	10	theme	macromolecular	966:979	arg1	diffusion					987:995	macromolecular probe diffusion	966:995	macromolecular probe diffusion	966:995	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	4	11	theme	Alginate	598:605	arg1	gels					607:610	MATERIAL AND METHODS Alginate gels	577:610	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M))	577:719	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	0	12	from	consequences	52:63	arg1	properties					89:98	the physico-chemical properties	68:98	the physico-chemical properties of alginate-containing biofilms	68:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	6	13	theme	stiffness	1145:1153	arg1	terms					1123:1127	terms	1123:1127	terms of gel density, stiffness, diffusion property, presence and state of the water molecules	1123:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	4	14	theme	METHODS	590:596	arg1	gels					607:610	MATERIAL AND METHODS Alginate gels	577:610	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M))	577:719	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	15	theme	mannuronate	698:708	arg1	M					717:717	M	717:717	M	717:717	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	15	theme	mannuronate	698:708	arg1	units					710:714	mannuronate units	698:714	mannuronate units (M)	698:718	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	6	16	theme	alginate	1047:1054	arg1	network					1056:1062	the alginate network	1043:1062	the alginate network	1043:1062	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	5	17	theme	Ca2+	947:950	arg1	displacement					952:963	Ca2+ displacement	947:963	Ca2+ displacement	947:963	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	4	18	from	units	673:677	arg1	enriched					650:657	enriched	650:657	enriched	650:657	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	1	19	theme	polymeric	186:194	arg1	EPS					208:210	EPS	208:210	EPS	208:210	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	19	theme	polymeric	186:194	arg1	substances					196:205	the main extracellular polymeric substances	163:205	the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	163:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	0	20	theme	biofilms	123:130	arg1	properties					89:98	the physico-chemical properties	68:98	the physico-chemical properties of alginate-containing biofilms	68:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	3	21	theme	alginate	519:526	arg1	network					543:549	the alginate macromolecular network	515:549	the alginate macromolecular network	515:549	In this study, we investigated how GS modifies the alginate macromolecular network and its microenvironment.
30304700	1	22	theme	substances	196:205	arg1	one					156:158	one	156:158	one	156:158	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	22	theme	substances	196:205	arg1	substances					196:205	the main extracellular polymeric substances	163:205	the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections	163:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	22	theme	substances	196:205	arg1	EPS					208:210	EPS	208:210	EPS	208:210	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	5	23	theme	pH	1001:1002	arg1	alteration					1004:1013	pH alteration	1001:1013	pH alteration	1001:1013	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	8	24	theme	macromolecular	1499:1512	arg1	target					1514:1519	a potential macromolecular target	1487:1519	a potential macromolecular target to improve anti-infectious therapies	1487:1556	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	6	25	theme	molecules	1208:1216	arg1	state					1189:1193	state	1189:1193	state	1189:1193	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	25	theme	molecules	1208:1216	arg1	presence					1176:1183	presence	1176:1183	presence	1176:1183	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	25	theme	molecules	1208:1216	arg1	stiffness					1145:1153	stiffness	1145:1153	stiffness	1145:1153	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	25	theme	molecules	1208:1216	arg1	property					1166:1173	diffusion property	1156:1173	diffusion property	1156:1173	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	25	theme	molecules	1208:1216	arg1	density					1136:1142	gel density	1132:1142	gel density	1132:1142	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	26	from	RESULTS	1016:1022	arg1	presence					1027:1034	presence	1027:1034	presence of GS	1027:1040	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	27	theme	gel	1132:1134	arg1	density					1136:1142	gel density	1132:1142	gel density	1132:1142	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	2	28	theme	pharmacological	370:384	arg1	activity					386:393	pharmacological activity	370:393	pharmacological activity	370:393	Gentamicin sulfate (GS) can strongly bind to alginate resulting in loss of pharmacological activity; however neither the mechanism nor its repercussion is fully understood.
30304700	1	29	theme	pulmonary	273:281	arg1	infections					283:292	pulmonary infections	273:292	pulmonary infections	273:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	6	30	theme	water	1202:1206	arg1	molecules					1208:1216	the water molecules	1198:1216	the water molecules	1198:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	4	31	from	enriched	650:657	arg1	G					680:680	G	680:680	G	680:680	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	31	from	enriched	650:657	arg1	units					673:677	guluronate units	662:677	guluronate units (G)	662:681	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	6	32	theme	GS	1039:1040	arg1	presence					1027:1034	presence	1027:1034	presence of GS	1027:1040	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	0	33	theme	sulfate	26:32	arg1	Interaction					0:10	Interaction	0:10	Interaction of gentamicin sulfate with alginate	0:46	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	0	33	theme	sulfate	26:32	arg1	consequences					52:63	consequences	52:63	consequences on the physico-chemical properties of alginate-containing biofilms	52:130	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	6	34	theme	property	1166:1173	arg1	terms					1123:1127	terms	1123:1127	terms of gel density, stiffness, diffusion property, presence and state of the water molecules	1123:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	5	35	theme	swelling	879:886	arg1	assay					888:892	swelling assay	879:892	swelling assay	879:892	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	5	36	theme	nuclear	919:925	arg1	resonance					936:944	nuclear magnetic resonance	919:944	nuclear magnetic resonance	919:944	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	7	37	theme	alterations	1256:1266	arg1	dependent					1280:1288	dependent	1280:1288	dependent	1280:1288	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	7	37	theme	alterations	1256:1266	arg1	intensity					1237:1245	the intensity	1233:1245	the intensity of those alterations	1233:1266	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	0	38	theme	gentamicin	15:24	arg1	sulfate					26:32	gentamicin sulfate	15:32	gentamicin sulfate	15:32	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	8	39	theme	alginate	1394:1401	arg1	importance					1380:1389	the importance	1376:1389	the importance of alginate as biofilm component, its pernicious role during antibiotherapy	1376:1465	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	8	40	theme	biofilm	1406:1412	arg1	component					1414:1422	biofilm component	1406:1422	biofilm component	1406:1422	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	8	40	theme	biofilm	1406:1412	arg1	role					1440:1443	its pernicious role	1425:1443	its pernicious role during antibiotherapy	1425:1465	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	5	41	theme	magnetic	927:934	arg1	resonance					936:944	nuclear magnetic resonance	919:944	nuclear magnetic resonance	919:944	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	4	42	theme	compositions	629:640	arg1	gels					607:610	MATERIAL AND METHODS Alginate gels	577:610	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M))	577:719	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	8	43	theme	anti-infectious	1532:1546	arg1	therapies					1548:1556	anti-infectious therapies	1532:1556	anti-infectious therapies	1532:1556	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	4	44	theme	guluronate	662:671	arg1	G					680:680	G	680:680	G	680:680	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	44	theme	guluronate	662:671	arg1	units					673:677	guluronate units	662:677	guluronate units (G)	662:681	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	45	theme	MATERIAL	577:584	arg1	gels					607:610	MATERIAL AND METHODS Alginate gels	577:610	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M))	577:719	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	46	theme	varying	769:775	arg1	times					777:781	varying times	769:781	varying times	769:781	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	8	47	dep	CONCLUSION	1343:1352	arg1	represent					1477:1485	represent	1477:1485	could represent a potential macromolecular target to improve anti-infectious therapies	1471:1556	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	8	47	dep	CONCLUSION	1343:1352	arg1	underline					1366:1374	underline	1366:1374	underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy	1366:1465	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	7	48	theme	polysaccharide	1297:1310	arg1	composition					1318:1328	the polysaccharide motif composition	1293:1328	the polysaccharide motif composition (ratio M/G)	1293:1340	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	7	48	theme	polysaccharide	1297:1310	arg1	M/G					1337:1339	ratio M/G	1331:1339	ratio M/G	1331:1339	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	6	49	theme	state	1189:1193	arg1	terms					1123:1127	terms	1123:1127	terms of gel density, stiffness, diffusion property, presence and state of the water molecules	1123:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	1	50	theme	BACKGROUND	133:142	arg1	Alginate					144:151	BACKGROUND Alginate	133:151	BACKGROUND Alginate	133:151	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	8	51	theme	pernicious	1429:1438	arg1	component					1414:1422	biofilm component	1406:1422	biofilm component	1406:1422	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	8	51	theme	pernicious	1429:1438	arg1	role					1440:1443	its pernicious role	1425:1443	its pernicious role during antibiotherapy	1425:1465	CONCLUSION Our results underline the importance of alginate as biofilm component, its pernicious role during antibiotherapy and could represent a potential macromolecular target to improve anti-infectious therapies.
30304700	4	52	dep	compositions	629:640	arg1	either					643:648	either	643:648	either	643:648	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	4	52	dep	compositions	629:640	arg1	enriched					686:693	enriched	686:693	enriched in mannuronate units (M)	686:718	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	6	53	theme	tremendous	1094:1103	arg1	reorganization					1105:1118	a tremendous reorganization	1092:1118	a tremendous reorganization	1092:1118	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	7	54	theme	motif	1312:1316	arg1	composition					1318:1328	the polysaccharide motif composition	1293:1328	the polysaccharide motif composition (ratio M/G)	1293:1340	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	7	54	theme	motif	1312:1316	arg1	M/G					1337:1339	ratio M/G	1331:1339	ratio M/G	1331:1339	We noted that the intensity of those alterations is directly dependent on the polysaccharide motif composition (ratio M/G).
30304700	6	55	theme	presence	1176:1183	arg1	terms					1123:1127	terms	1123:1127	terms of gel density, stiffness, diffusion property, presence and state of the water molecules	1123:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	0	56	with	Interaction	0:10	arg1	alginate					39:46	alginate	39:46	alginate	39:46	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	1	57	theme	Cystic	228:233	arg1	CF					245:246	CF	245:246	CF	245:246	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	1	57	theme	Cystic	228:233	arg1	Fibrosis					235:242	Cystic Fibrosis	228:242	Cystic Fibrosis (CF) patients suffering from pulmonary infections	228:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	6	58	theme	density	1136:1142	arg1	terms					1123:1127	terms	1123:1127	terms of gel density, stiffness, diffusion property, presence and state of the water molecules	1123:1216	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	4	59	dep	either	643:648	arg1	enriched					650:657	enriched	650:657	enriched	650:657	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	3	60	theme	macromolecular	528:541	arg1	network					543:549	the alginate macromolecular network	515:549	the alginate macromolecular network	515:549	In this study, we investigated how GS modifies the alginate macromolecular network and its microenvironment.
30304700	0	61	with	consequences	52:63	arg1	alginate					39:46	alginate	39:46	alginate	39:46	Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms.
30304700	5	62	theme	calorimetry	895:905	arg1	techniques					907:916	calorimetry techniques	895:916	calorimetry techniques	895:916	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	1	63	theme	Fibrosis	235:242	arg1	patients					249:256	Cystic Fibrosis (CF) patients	228:256	Cystic Fibrosis (CF) patients suffering from pulmonary infections	228:292	BACKGROUND Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections.
30304700	5	64	theme	probe	981:985	arg1	diffusion					987:995	macromolecular probe diffusion	966:995	macromolecular probe diffusion	966:995	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
30304700	6	65	from	network	1056:1062	arg1	presence					1027:1034	presence	1027:1034	presence of GS	1027:1040	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	2	66	theme	activity	386:393	arg1	loss					362:365	loss	362:365	loss of pharmacological activity	362:393	Gentamicin sulfate (GS) can strongly bind to alginate resulting in loss of pharmacological activity; however neither the mechanism nor its repercussion is fully understood.
30304700	4	67	theme	different	619:627	arg1	compositions					629:640	two different compositions	615:640	two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M))	615:719	MATERIAL AND METHODS Alginate gels of two different compositions (either enriched in guluronate units (G) or enriched in mannuronate units (M)) were crosslinked with Ca2+ and exposed to GS at varying times and concentrations.
30304700	6	68	theme	diffusion	1156:1164	arg1	property					1166:1173	diffusion property	1156:1173	diffusion property	1156:1173	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	6	69	from	environment	1072:1082	arg1	presence					1027:1034	presence	1027:1034	presence of GS	1027:1040	RESULTS In presence of GS, the alginate network and its environment undergo a tremendous reorganization in terms of gel density, stiffness, diffusion property, presence and state of the water molecules.
30304700	5	70	theme	mechanical	861:870	arg1	tests					872:876	mechanical tests	861:876	mechanical tests	861:876	The complexes formed were characterized via turbidimetry, mechanical tests, swelling assay, calorimetry techniques, nuclear magnetic resonance, Ca2+ displacement, macromolecular probe diffusion and pH alteration.
29981828	5	0	dep	%	638:638	arg1	6					629:629	6	629:629	6	629:629	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	1	from	biodegradability	695:710	arg1	properties					683:692	the mechanical (UTM) and thermal (TGA) properties	644:692	the mechanical (UTM) and thermal (TGA) properties	644:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	2	theme	CS	622:623	arg1	influence					609:617	The influence	605:617	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties	605:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	2	theme	CS	622:623	arg1	flammability					736:747	flammability	736:747	flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites	736:826	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	2	theme	CS	622:623	arg1	biodegradability					695:710	biodegradability	695:710	biodegradability (soil burial test)	695:729	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	2	theme	CS	622:623	arg1	test					725:728	soil burial test	713:728	soil burial test	713:728	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	2	3	theme	starch	333:338	arg1	form					311:314	the form	307:314	the form of thermoplastic starch prepared via mechano ball milling	307:372	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	6	4	theme	dense	1124:1128	arg1	char					1130:1133	the dense char	1120:1133	the dense char formed by the carbonaceous agent CS	1120:1169	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	2	5	theme	thermoplastic	319:331	arg1	starch					333:338	thermoplastic starch	319:338	thermoplastic starch prepared via mechano ball milling	319:372	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	4	6	theme	reinforcements	554:567	arg1	compatibility					510:522	Good compatibility	505:522	Good compatibility	505:522	Good compatibility and homogeneous dispersion of reinforcements was corroborated using FESEM (EDX).
29981828	4	6	theme	reinforcements	554:567	arg1	dispersion					540:549	homogeneous dispersion	528:549	homogeneous dispersion	528:549	Good compatibility and homogeneous dispersion of reinforcements was corroborated using FESEM (EDX).
29981828	5	7	theme	mechanical	648:657	arg1	properties					683:692	the mechanical (UTM) and thermal (TGA) properties	644:692	the mechanical (UTM) and thermal (TGA) properties	644:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	8	theme	composites	1042:1051	arg1	withstandability					995:1010	remarkable withstandability	984:1010	remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate	984:1088	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	8	theme	composites	1042:1051	arg1	LOI = 40					1025:1032	LOI = 40	1025:1032	LOI = 40	1025:1032	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	9	from	16.45	886:890	arg1	improvement					849:859	An improvement	846:859	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy	846:975	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	9	from	16.45	886:890	arg1	strength					872:879	tensile strength	864:879	tensile strength from 16.45	864:890	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	7	10	theme	composites	1266:1275	arg1	durability					1248:1257	longer durability	1241:1257	longer durability of the composites	1241:1275	A delay in biodegradation was observed for CS composites, indicating longer durability of the composites.
29981828	2	11	theme	typical	271:277	arg1	process					279:285	a typical process	269:285	a typical process	269:285	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	3	12	theme	composites	469:478	arg1	nature					455:460	The nature	451:460	The nature of the composites	451:478	The nature of the composites was analysed using FTIR.
29981828	7	13	from	delay	1174:1178	arg1	biodegradation					1183:1196	biodegradation	1183:1196	biodegradation	1183:1196	A delay in biodegradation was observed for CS composites, indicating longer durability of the composites.
29981828	6	14	with	composites	1042:1051	arg1	self-annihilate					1074:1088	flame and flame self-annihilate	1058:1088	self-annihilate	1074:1088	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	15	theme	limited	782:788	arg1	LOI					804:806	LOI	804:806	LOI	804:806	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	15	theme	limited	782:788	arg1	index					797:801	limited oxygen index	782:801	limited oxygen index (LOI)	782:807	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	15	theme	limited	782:788	arg1	test					769:772	horizontal burning test	750:772	horizontal burning test (UL94)	750:779	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	16	theme	burning	761:767	arg1	index					797:801	limited oxygen index	782:801	limited oxygen index (LOI)	782:807	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	16	theme	burning	761:767	arg1	UL94					775:778	UL94	775:778	UL94	775:778	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	16	theme	burning	761:767	arg1	test					769:772	horizontal burning test	750:772	horizontal burning test (UL94)	750:779	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	17	theme	@	931:931	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	17	theme	@	931:931	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	18	theme	horizontal	750:759	arg1	index					797:801	limited oxygen index	782:801	limited oxygen index (LOI)	782:807	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	18	theme	horizontal	750:759	arg1	UL94					775:778	UL94	775:778	UL94	775:778	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	18	theme	horizontal	750:759	arg1	test					769:772	horizontal burning test	750:772	horizontal burning test (UL94)	750:779	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	19	from	influence	609:617	arg1	properties					683:692	the mechanical (UTM) and thermal (TGA) properties	644:692	the mechanical (UTM) and thermal (TGA) properties	644:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	7	20	theme	longer	1241:1246	arg1	durability					1248:1257	longer durability	1241:1257	longer durability of the composites	1241:1275	A delay in biodegradation was observed for CS composites, indicating longer durability of the composites.
29981828	2	21	theme	ball	361:364	arg1	milling					366:372	mechano ball milling	353:372	mechano ball milling	353:372	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	5	22	theme	soil	713:716	arg1	biodegradability					695:710	biodegradability	695:710	biodegradability (soil burial test)	695:729	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	22	theme	soil	713:716	arg1	test					725:728	soil burial test	713:728	soil burial test	713:728	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	4	23	theme	homogeneous	528:538	arg1	dispersion					540:549	homogeneous dispersion	528:549	homogeneous dispersion	528:549	Good compatibility and homogeneous dispersion of reinforcements was corroborated using FESEM (EDX).
29981828	6	24	from	improvement	849:859	arg1	strength					872:879	tensile strength	864:879	tensile strength from 16.45	864:890	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	24	from	improvement	849:859	arg1	16.45					886:890	16.45	886:890	16.45	886:890	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	2	25	theme	mechano	353:359	arg1	milling					366:372	mechano ball milling	353:372	mechano ball milling	353:372	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	5	26	theme	burial	718:723	arg1	biodegradability					695:710	biodegradability	695:710	biodegradability (soil burial test)	695:729	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	26	theme	burial	718:723	arg1	test					725:728	soil burial test	713:728	soil burial test	713:728	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	27	theme	%	929:929	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	27	theme	%	929:929	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	1	28	dep	natural	176:182	arg1	available					192:200	available	192:200	available	192:200	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	6	29	theme	thermal	906:912	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	29	theme	thermal	906:912	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	30	theme	oxygen	790:795	arg1	LOI					804:806	LOI	804:806	LOI	804:806	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	30	theme	oxygen	790:795	arg1	index					797:801	limited oxygen index	782:801	limited oxygen index (LOI)	782:807	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	30	theme	oxygen	790:795	arg1	test					769:772	horizontal burning test	750:772	horizontal burning test (UL94)	750:779	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	31	theme	flame	960:964	arg1	retardancy					966:975	flame retardancy	960:975	flame retardancy	960:975	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	7	32	theme	CS	1215:1216	arg1	composites					1218:1227	CS composites	1215:1227	CS composites	1215:1227	A delay in biodegradation was observed for CS composites, indicating longer durability of the composites.
29981828	5	33	dep	mechanical	648:657	arg1	UTM					660:662	UTM	660:662	UTM	660:662	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	33	dep	mechanical	648:657	arg1	TGA					678:680	TGA	678:680	TGA	678:680	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	0	34	theme	starch/chitosan/flax	36:55	arg1	characterisation					16:31	characterisation	16:31	characterisation	16:31	Fabrication and characterisation of starch/chitosan/flax fabric green flame-retardant composites.
29981828	0	34	theme	starch/chitosan/flax	36:55	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterisation of starch/chitosan/flax fabric green flame-retardant composites.
29981828	6	35	theme	agent	1162:1166	arg1	CS					1168:1169	the carbonaceous agent CS	1145:1169	the carbonaceous agent CS	1145:1169	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	36	theme	flame	1068:1072	arg1	self-annihilate					1074:1088	flame and flame self-annihilate	1058:1088	self-annihilate	1074:1088	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	37	theme	10 wt	924:928	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	37	theme	10 wt	924:928	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	0	38	theme	flame-retardant	70:84	arg1	composites					86:95	green flame-retardant composites	64:95	green flame-retardant composites	64:95	Fabrication and characterisation of starch/chitosan/flax fabric green flame-retardant composites.
29981828	6	39	theme	carbonaceous	1149:1160	arg1	CS					1168:1169	the carbonaceous agent CS	1145:1169	the carbonaceous agent CS	1145:1169	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	40	theme	composites	817:826	arg1	influence					609:617	The influence	605:617	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties	605:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	40	theme	composites	817:826	arg1	flammability					736:747	flammability	736:747	flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites	736:826	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	40	theme	composites	817:826	arg1	biodegradability					695:710	biodegradability	695:710	biodegradability (soil burial test)	695:729	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	40	theme	composites	817:826	arg1	test					725:728	soil burial test	713:728	soil burial test	713:728	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	41	from	flammability	736:747	arg1	properties					683:692	the mechanical (UTM) and thermal (TGA) properties	644:692	the mechanical (UTM) and thermal (TGA) properties	644:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	1	42	theme	eco-friendly	135:146	arg1	bio-composites					148:161	eco-friendly bio-composites	135:161	eco-friendly bio-composites	135:161	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	0	43	theme	green	64:68	arg1	composites					86:95	green flame-retardant composites	64:95	green flame-retardant composites	64:95	Fabrication and characterisation of starch/chitosan/flax fabric green flame-retardant composites.
29981828	6	44	theme	stability	914:922	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	44	theme	stability	914:922	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	45	theme	flame	1058:1062	arg1	self-annihilate					1074:1088	flame and flame self-annihilate	1058:1088	self-annihilate	1074:1088	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	1	46	theme	bio-composites	148:161	arg1	fabrication					120:130	the fabrication	116:130	the fabrication of eco-friendly bio-composites	116:161	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	4	47	theme	Good	505:508	arg1	compatibility					510:522	Good compatibility	505:522	Good compatibility	505:522	Good compatibility and homogeneous dispersion of reinforcements was corroborated using FESEM (EDX).
29981828	5	48	theme	&	632:632	arg1	CS					622:623	CS	622:623	CS (3, 6, & 9 wt%)	622:639	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	48	theme	&	632:632	arg1	%					638:638	& 9 wt%	632:638	& 9 wt%	632:638	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	2	49	with	compression	423:433	arg1	FF					447:448	FF	447:448	FF	447:448	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	2	49	with	compression	423:433	arg1	CS					440:441	CS	440:441	CS	440:441	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	6	50	theme	remarkable	984:993	arg1	withstandability					995:1010	remarkable withstandability	984:1010	remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate	984:1088	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	50	theme	remarkable	984:993	arg1	LOI = 40					1025:1032	LOI = 40	1025:1032	LOI = 40	1025:1032	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	51	theme	9 wt	634:637	arg1	CS					622:623	CS	622:623	CS (3, 6, & 9 wt%)	622:639	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	51	theme	9 wt	634:637	arg1	%					638:638	& 9 wt%	632:638	& 9 wt%	632:638	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	52	dep	showed	977:982	arg1	speculated					1095:1104	speculated	1095:1104	showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS	977:1169	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	5	53	dep	flammability	736:747	arg1	index					797:801	limited oxygen index	782:801	limited oxygen index (LOI)	782:807	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	53	dep	flammability	736:747	arg1	UL94					775:778	UL94	775:778	UL94	775:778	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	5	53	dep	flammability	736:747	arg1	test					769:772	horizontal burning test	750:772	horizontal burning test (UL94)	750:779	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	1	54	theme	natural	176:182	arg1	chitosan					230:237	chitosan	230:237	chitosan (CS)	230:242	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	1	54	theme	natural	176:182	arg1	biopolymers					202:212	natural, widely available biopolymers	176:212	natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF)	176:263	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	1	54	theme	natural	176:182	arg1	starch					222:227	starch	222:227	starch	222:227	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	1	54	theme	natural	176:182	arg1	fabric					253:258	flax fabric	248:258	flax fabric (FF)	248:263	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	2	55	used	used	299:302	arg2	starch					288:293	starch	288:293	starch	288:293	In a typical process, starch was used in the form of thermoplastic starch prepared via mechano ball milling and subsequently, composites were fabricated via compression with CS and FF.
29981828	1	56	theme	flax	248:251	arg1	FF					261:262	FF	261:262	FF	261:262	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	1	56	theme	flax	248:251	arg1	fabric					253:258	flax fabric	248:258	flax fabric (FF)	248:263	The study reveals the fabrication of eco-friendly bio-composites by employing natural, widely available biopolymers such as starch, chitosan (CS) and flax fabric (FF).
29981828	5	57	theme	thermal	669:675	arg1	properties					683:692	the mechanical (UTM) and thermal (TGA) properties	644:692	the mechanical (UTM) and thermal (TGA) properties	644:692	The influence of CS (3, 6, & 9 wt%) on the mechanical (UTM) and thermal (TGA) properties, biodegradability (soil burial test), and flammability (horizontal burning test (UL94), limited oxygen index (LOI)) of the composites was investigated.
29981828	6	58	theme	N2	941:942	arg1	800 °C					933:938	thermal stability 10 wt% @ 800 °C	906:938	thermal stability 10 wt% @ 800 °C (N2 atmosphere)	906:954	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	58	theme	N2	941:942	arg1	atmosphere					944:953	N2 atmosphere	941:953	N2 atmosphere	941:953	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
29981828	6	59	theme	tensile	864:870	arg1	strength					872:879	tensile strength	864:879	tensile strength from 16.45	864:890	An improvement in tensile strength from 16.45 to 20.78 MPa, thermal stability 10 wt% @ 800 °C (N2 atmosphere) and flame retardancy showed remarkable withstandability (UL94 = Vo & LOI = 40) of the composites with flame and flame self-annihilate were speculated to arise from the dense char formed by the carbonaceous agent CS.
31801844	7	0	theme	chemical	1341:1348	arg1	structure					1350:1358	the chemical structure	1337:1358	the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations	1337:1528	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	10	1	theme	LTA-deficient	2151:2163	arg1	strain					2165:2170	an LTA-deficient strain	2148:2170	an LTA-deficient strain of S. gordonii	2148:2185	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	11	2	theme	eukaryotic	2486:2495	arg1	cells					2497:2501	eukaryotic cells	2486:2501	eukaryotic cells	2486:2501	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	6	3	theme	bacteria	1305:1312	arg1	species					1280:1286	other species	1274:1286	other species of Gram-positive bacteria	1274:1312	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	10	4	used	utilized	2139:2146	arg2	we					2136:2137	we	2136:2137	we	2136:2137	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	3	5	theme	surface	507:513	arg1	presentation					523:534	surface protein presentation	507:534	surface protein presentation	507:534	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	8	6	theme	gordonii	1545:1552	arg1	LTA					1554:1556	the S. gordonii LTA	1538:1556	the S. gordonii LTA	1538:1556	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	10	7	theme	gordonii	2178:2185	arg1	strain					2165:2170	an LTA-deficient strain	2148:2170	an LTA-deficient strain of S. gordonii	2148:2185	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	8	8	theme	abundant	1736:1743	arg1	polymer					1745:1751	an abundant polymer	1733:1751	an abundant polymer found on the surface of Gram-positive bacteria	1733:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	8	theme	abundant	1736:1743	arg1	acid					1719:1722	lipoteichoic acid	1706:1722	lipoteichoic acid (LTA)	1706:1728	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	1	9	theme	cell	169:172	arg1	envelope					174:181	the Gram-positive bacterial cell envelope	141:181	the Gram-positive bacterial cell envelope	141:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	10	10	theme	LTA-deficient	1964:1976	arg1	strains					1978:1984	LTA-deficient strains	1964:1984	LTA-deficient strains	1964:1984	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	4	11	theme	label-free	645:654	arg1	spectroscopy					661:672	label-free mass spectroscopy	645:672	label-free mass spectroscopy	645:672	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	8	12	dep	half-century	1688:1699	arg1	polymer					1745:1751	an abundant polymer	1733:1751	an abundant polymer found on the surface of Gram-positive bacteria	1733:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	12	dep	half-century	1688:1699	arg1	acid					1719:1722	lipoteichoic acid	1706:1722	lipoteichoic acid (LTA)	1706:1728	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	11	13	theme	envelope	2409:2416	arg1	homeostasis					2418:2428	cell envelope homeostasis	2404:2428	cell envelope homeostasis	2404:2428	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	4	14	theme	gordonii	616:623	arg1	strains					602:608	the wild-type and LTA-deficient strains	570:608	the wild-type and LTA-deficient strains of S. gordonii	570:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	6	15	theme	other	1178:1182	arg1	species					1194:1200	other bacterial species	1178:1200	other bacterial species	1178:1200	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	5	16	theme	envelope	925:932	arg1	response					941:948	envelope stress response	925:948	envelope stress response	925:948	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	16	theme	envelope	925:932	arg1	DegP					919:922	(iv) DegP	914:922	(iv) DegP (envelope stress response)	914:949	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	1	17	theme	many	204:207	arg1	species					209:215	many species	204:215	many species	204:215	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	7	18	theme	I	1465:1465	arg1	LTA					1467:1469	a complex type I LTA	1450:1469	a complex type I LTA	1450:1469	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	6	19	theme	biofilm	1076:1082	arg1	formation					1084:1092	biofilm formation	1076:1092	biofilm formation	1076:1092	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	8	20	theme	bacteria	1791:1798	arg1	surface					1766:1772	the surface	1762:1772	the surface of Gram-positive bacteria	1762:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	6	21	from	loss	1259:1262	arg1	species					1280:1286	other species	1274:1286	other species of Gram-positive bacteria	1274:1312	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	8	22	theme	surface-associated	1617:1634	arg1	proteins					1636:1643	cell surface-associated proteins	1612:1643	cell surface-associated proteins	1612:1643	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	7	23	theme	complex	1452:1458	arg1	LTA					1467:1469	a complex type I LTA	1450:1469	a complex type I LTA	1450:1469	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	6	24	theme	homeostasis	1063:1073	arg1	observations					1025:1036	our observations	1021:1036	our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells	1021:1126	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	1	25	theme	abundant	121:128	arg1	acid					104:107	Lipoteichoic acid	91:107	Lipoteichoic acid (LTA)	91:113	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	1	25	theme	abundant	121:128	arg1	polymer					130:136	an abundant polymer	118:136	an abundant polymer of the Gram-positive bacterial cell envelope	118:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	6	26	theme	cell	1049:1052	arg1	homeostasis					1063:1073	altered cell envelope homeostasis	1041:1073	altered cell envelope homeostasis	1041:1073	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	10	27	theme	commensal	2062:2070	arg1	gordonii					2086:2093	the human oral commensal Streptococcus gordonii	2047:2093	the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2047:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	0	28	theme	Surface	63:69	arg1	Biogenesis					79:88	Surface Protein Biogenesis	63:88	Surface Protein Biogenesis	63:88	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	2	29	theme	exact	230:234	arg1	function					236:243	the exact function	226:243	the exact function of LTA	226:250	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	2	30	from	loss	277:280	arg1	species					297:303	some species	292:303	some species	292:303	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	11	31	theme	many	2349:2352	arg1	proteins					2378:2385	many cell surface-associated proteins	2349:2385	many cell surface-associated proteins	2349:2385	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	9	32	theme	Gram-positive	1852:1864	arg1	species					1866:1872	many Gram-positive species	1847:1872	many Gram-positive species	1847:1872	Although LTA is essential for the survival of many Gram-positive species, knowledge of how LTA contributes to bacterial physiology has remained elusive.
31801844	1	33	theme	Gram-positive	145:157	arg1	envelope					174:181	the Gram-positive bacterial cell envelope	141:181	the Gram-positive bacterial cell envelope	141:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	5	34	theme	many	717:720	arg1	proteins					722:729	many proteins	717:729	many proteins	717:729	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	35	dep	FtsE	825:828	arg1	ii					821:822	ii	821:822	ii	821:822	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	8	36	theme	lipoteichoic	1706:1717	arg1	polymer					1745:1751	an abundant polymer	1733:1751	an abundant polymer found on the surface of Gram-positive bacteria	1733:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	36	theme	lipoteichoic	1706:1717	arg1	LTA					1725:1727	LTA	1725:1727	LTA	1725:1727	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	36	theme	lipoteichoic	1706:1717	arg1	acid					1719:1722	lipoteichoic acid	1706:1722	lipoteichoic acid (LTA)	1706:1728	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	11	37	theme	surface-associated	2359:2376	arg1	proteins					2378:2385	many cell surface-associated proteins	2349:2385	many cell surface-associated proteins	2349:2385	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	3	38	theme	LTA-deficient	382:394	arg1	strain					396:401	a viable LTA-deficient strain	373:401	a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii	373:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	0	39	theme	Streptococcus	0:12	arg1	Acid					43:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	3	40	theme	commensal	421:429	arg1	gordonii					445:452	the human oral commensal Streptococcus gordonii	406:452	the human oral commensal Streptococcus gordonii	406:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	0	41	theme	Type	23:26	arg1	Acid					43:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	7	42	theme	multiple	1487:1494	arg1	d-alanylations					1496:1509	multiple d-alanylations	1487:1509	multiple d-alanylations	1487:1509	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	3	43	theme	human	410:414	arg1	gordonii					445:452	the human oral commensal Streptococcus gordonii	406:452	the human oral commensal Streptococcus gordonii	406:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	0	44	theme	Lipoteichoic	30:41	arg1	Acid					43:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	10	45	theme	human	2051:2055	arg1	gordonii					2086:2093	the human oral commensal Streptococcus gordonii	2047:2093	the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2047:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	6	46	from	changes	958:964	arg1	presentation					990:1001	cell surface protein presentation	969:1001	cell surface protein presentation	969:1001	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	6	47	theme	cell	969:972	arg1	presentation					990:1001	cell surface protein presentation	969:1001	cell surface protein presentation	969:1001	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	3	48	theme	gordonii	445:452	arg1	strain					396:401	a viable LTA-deficient strain	373:401	a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii	373:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	10	49	theme	oral	2057:2060	arg1	gordonii					2086:2093	the human oral commensal Streptococcus gordonii	2047:2093	the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2047:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	5	50	theme	cell	870:873	arg1	biosynthesis					880:891	cell wall biosynthesis	870:891	cell wall biosynthesis	870:891	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	51	theme	biofilm	800:806	arg1	SGO_0707					789:796	SGO_0707	789:796	SGO_0707	789:796	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	51	theme	biofilm	800:806	arg1	formation					808:816	biofilm formation	800:816	biofilm formation	800:816	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	11	52	from	interactions	2444:2455	arg1	biofilms					2460:2467	biofilms	2460:2467	biofilms	2460:2467	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	4	53	theme	wall	542:545	arg1	fractions					547:555	Cell wall fractions	537:555	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii	537:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	6	54	theme	Gram-positive	1291:1303	arg1	bacteria					1305:1312	Gram-positive bacteria	1291:1312	Gram-positive bacteria	1291:1312	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	6	55	theme	eukaryotic	1111:1120	arg1	cells					1122:1126	eukaryotic cells	1111:1126	eukaryotic cells	1111:1126	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	3	56	theme	important	489:497	arg1	role					499:502	an important role	486:502	an important role	486:502	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	8	57	theme	S.	1542:1543	arg1	LTA					1554:1556	the S. gordonii LTA	1538:1556	the S. gordonii LTA	1538:1556	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	1	58	theme	bacterial	159:167	arg1	envelope					174:181	the Gram-positive bacterial cell envelope	141:181	the Gram-positive bacterial cell envelope	141:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	6	59	theme	adhesion	1099:1106	arg1	observations					1025:1036	our observations	1021:1036	our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells	1021:1126	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	2	60	theme	LTA	285:287	arg1	loss					277:280	loss	277:280	loss of LTA in some species	277:303	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	1	61	theme	envelope	174:181	arg1	acid					104:107	Lipoteichoic acid	91:107	Lipoteichoic acid (LTA)	91:113	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	1	61	theme	envelope	174:181	arg1	polymer					130:136	an abundant polymer	118:136	an abundant polymer of the Gram-positive bacterial cell envelope	118:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	5	62	dep	SspA	755:758	arg1	SGO_0707					789:796	SGO_0707	789:796	SGO_0707	789:796	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	62	dep	SspA	755:758	arg1	formation					808:816	biofilm formation	800:816	biofilm formation	800:816	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	62	dep	SspA	755:758	arg1	i					752:752	i	752:752	i	752:752	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	10	63	theme	Gram-positive	2014:2026	arg1	species					2028:2034	some Gram-positive species	2009:2034	some Gram-positive species	2009:2034	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	10	63	theme	Gram-positive	2014:2026	arg1	gordonii					2086:2093	the human oral commensal Streptococcus gordonii	2047:2093	the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2047:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	3	64	theme	protein	515:521	arg1	presentation					523:534	surface protein presentation	507:534	surface protein presentation	507:534	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	6	65	with	binding	1147:1153	arg1	species					1194:1200	other bacterial species	1178:1200	other bacterial species	1178:1200	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	12	66	theme	Gram-positive	2566:2578	arg1	bacteria					2580:2587	Gram-positive bacteria	2566:2587	Gram-positive bacteria	2566:2587	These data may broadly reflect a physiological role of LTA in Gram-positive bacteria.
31801844	11	67	theme	cell	2404:2407	arg1	homeostasis					2418:2428	cell envelope homeostasis	2404:2428	cell envelope homeostasis	2404:2428	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	6	68	theme	protein	982:988	arg1	presentation					990:1001	cell surface protein presentation	969:1001	cell surface protein presentation	969:1001	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	6	69	theme	bacterial	1184:1192	arg1	species					1194:1200	other bacterial species	1178:1200	other bacterial species	1178:1200	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	6	70	with	coaggregation	1159:1171	arg1	species					1194:1200	other bacterial species	1178:1200	other bacterial species	1178:1200	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	7	71	theme	type	1460:1463	arg1	LTA					1467:1469	a complex type I LTA	1450:1469	a complex type I LTA	1450:1469	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	6	72	theme	formation	1084:1092	arg1	observations					1025:1036	our observations	1021:1036	our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells	1021:1126	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	5	73	theme	stress	934:939	arg1	response					941:948	envelope stress response	925:948	envelope stress response	925:948	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	73	theme	stress	934:939	arg1	DegP					919:922	(iv) DegP	914:922	(iv) DegP (envelope stress response)	914:949	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	11	74	theme	cell-to-cell	2431:2442	arg1	interactions					2444:2455	cell-to-cell interactions	2431:2455	cell-to-cell interactions in biofilms	2431:2467	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	12	75	theme	physiological	2537:2549	arg1	role					2551:2554	a physiological role	2535:2554	a physiological role of LTA in Gram-positive bacteria	2535:2587	These data may broadly reflect a physiological role of LTA in Gram-positive bacteria.
31801844	2	76	theme	LTA	248:250	arg1	function					236:243	the exact function	226:243	the exact function of LTA	226:250	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	8	77	theme	cell	1612:1615	arg1	proteins					1636:1643	cell surface-associated proteins	1612:1643	cell surface-associated proteins	1612:1643	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	5	78	theme	cell	831:834	arg1	FtsE					825:828	(ii) FtsE	820:828	(ii) FtsE (cell division)	820:844	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	78	theme	cell	831:834	arg1	division					836:843	cell division	831:843	cell division	831:843	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	12	79	theme	LTA	2559:2561	arg1	role					2551:2554	a physiological role	2535:2554	a physiological role of LTA in Gram-positive bacteria	2535:2587	These data may broadly reflect a physiological role of LTA in Gram-positive bacteria.
31801844	6	80	theme	envelope	1054:1061	arg1	homeostasis					1063:1073	altered cell envelope homeostasis	1041:1073	altered cell envelope homeostasis	1041:1073	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	10	81	theme	Streptococcus	2072:2084	arg1	gordonii					2086:2093	the human oral commensal Streptococcus gordonii	2047:2093	the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2047:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	5	82	dep	Pbp1a	853:857	arg1	iii					848:850	iii	848:850	iii	848:850	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	82	dep	Pbp1a	853:857	arg1	remodeling					897:906	remodeling	897:906	remodeling	897:906	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	82	dep	Pbp1a	853:857	arg1	biosynthesis					880:891	cell wall biosynthesis	870:891	cell wall biosynthesis	870:891	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	0	83	theme	Protein	71:77	arg1	Biogenesis					79:88	Surface Protein Biogenesis	63:88	Surface Protein Biogenesis	63:88	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	4	84	attach	derived	557:563	arg1	strains					602:608	the wild-type and LTA-deficient strains	570:608	the wild-type and LTA-deficient strains of S. gordonii	570:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	4	84	attach	derived	557:563	arg2	fractions					547:555	Cell wall fractions	537:555	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii	537:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	8	85	theme	proteins	1636:1643	arg1	presentation					1596:1607	presentation	1596:1607	presentation	1596:1607	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	85	theme	proteins	1636:1643	arg1	expression					1581:1590	expression	1581:1590	expression	1581:1590	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	6	86	theme	altered	1041:1047	arg1	homeostasis					1063:1073	altered cell envelope homeostasis	1041:1073	altered cell envelope homeostasis	1041:1073	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	9	87	theme	many	1847:1850	arg1	species					1866:1872	many Gram-positive species	1847:1872	many Gram-positive species	1847:1872	Although LTA is essential for the survival of many Gram-positive species, knowledge of how LTA contributes to bacterial physiology has remained elusive.
31801844	4	88	theme	mass	656:659	arg1	spectroscopy					661:672	label-free mass spectroscopy	645:672	label-free mass spectroscopy	645:672	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	10	89	dep	gordonii	2086:2093	arg1	is					2128:2129	is	2128:2129	is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria	2128:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	10	90	theme	Gram-positive	2238:2250	arg1	bacteria					2252:2259	Gram-positive bacteria	2238:2259	Gram-positive bacteria	2238:2259	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	9	91	theme	species	1866:1872	arg1	survival					1835:1842	the survival	1831:1842	the survival of many Gram-positive species	1831:1872	Although LTA is essential for the survival of many Gram-positive species, knowledge of how LTA contributes to bacterial physiology has remained elusive.
31801844	11	92	theme	proteins	2378:2385	arg1	presentation					2333:2344	the presentation	2329:2344	the presentation of many cell surface-associated proteins	2329:2385	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	5	93	theme	proteins	722:729	arg1	abundances					703:712	the abundances	699:712	the abundances of many proteins	699:729	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	11	94	theme	cell	2354:2357	arg1	proteins					2378:2385	many cell surface-associated proteins	2349:2385	many cell surface-associated proteins	2349:2385	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	2	95	theme	cell	352:355	arg1	division					357:364	cell division	352:364	cell division	352:364	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	0	96	theme	gordonii	14:21	arg1	Acid					43:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid	0:46	Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis.
31801844	1	97	theme	Lipoteichoic	91:102	arg1	acid					104:107	Lipoteichoic acid	91:107	Lipoteichoic acid (LTA)	91:113	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	1	97	theme	Lipoteichoic	91:102	arg1	polymer					130:136	an abundant polymer	118:136	an abundant polymer of the Gram-positive bacterial cell envelope	118:181	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	1	97	theme	Lipoteichoic	91:102	arg1	LTA					110:112	LTA	110:112	LTA	110:112	Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species.
31801844	8	98	theme	Gram-positive	1777:1789	arg1	bacteria					1791:1798	Gram-positive bacteria	1777:1798	Gram-positive bacteria	1777:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	10	99	theme	research	2119:2126	arg1	significance					2099:2110	The significance	2095:2110	The significance of our research	2095:2126	Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria.
31801844	6	100	theme	other	1274:1278	arg1	species					1280:1286	other species	1274:1286	other species of Gram-positive bacteria	1274:1312	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	11	101	from	adhesion	2474:2481	arg1	biofilms					2460:2467	biofilms	2460:2467	biofilms	2460:2467	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	4	102	theme	Cell	537:540	arg1	fractions					547:555	Cell wall fractions	537:555	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii	537:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	9	103	theme	bacterial	1911:1919	arg1	physiology					1921:1930	bacterial physiology	1911:1930	bacterial physiology	1911:1930	Although LTA is essential for the survival of many Gram-positive species, knowledge of how LTA contributes to bacterial physiology has remained elusive.
31801844	11	104	theme	important	2311:2319	arg1	role					2321:2324	an important role	2308:2324	an important role	2308:2324	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	3	105	theme	Streptococcus	431:443	arg1	gordonii					445:452	the human oral commensal Streptococcus gordonii	406:452	the human oral commensal Streptococcus gordonii	406:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	6	106	theme	LTA	1267:1269	arg1	loss					1259:1262	the loss	1255:1262	the loss of LTA in other species of Gram-positive bacteria	1255:1312	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	8	107	located	found	1753:1757	arg2	polymer					1745:1751	an abundant polymer	1733:1751	an abundant polymer found on the surface of Gram-positive bacteria	1733:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	107	located	found	1753:1757	arg2	acid					1719:1722	lipoteichoic acid	1706:1722	lipoteichoic acid (LTA)	1706:1728	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	8	107	located	found	1753:1757	arg1	surface					1766:1772	the surface	1762:1772	the surface of Gram-positive bacteria	1762:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	3	108	theme	viable	375:380	arg1	strain					396:401	a viable LTA-deficient strain	373:401	a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii	373:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	4	109	theme	LTA-deficient	588:600	arg1	strains					602:608	the wild-type and LTA-deficient strains	570:608	the wild-type and LTA-deficient strains of S. gordonii	570:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	3	110	theme	oral	416:419	arg1	gordonii					445:452	the human oral commensal Streptococcus gordonii	406:452	the human oral commensal Streptococcus gordonii	406:452	Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii, we demonstrated that LTA plays an important role in surface protein presentation.
31801844	6	111	theme	surface	974:980	arg1	presentation					990:1001	cell surface protein presentation	969:1001	cell surface protein presentation	969:1001	These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria.
31801844	12	112	from	role	2551:2554	arg1	bacteria					2580:2587	Gram-positive bacteria	2566:2587	Gram-positive bacteria	2566:2587	These data may broadly reflect a physiological role of LTA in Gram-positive bacteria.
31801844	7	113	theme	LTA	1367:1369	arg1	structure					1350:1358	the chemical structure	1337:1358	the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations	1337:1528	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
31801844	8	114	dep	functions.IMPORTANCE	1649:1668	arg1	Discovered					1670:1679	Discovered	1670:1679	functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria	1649:1798	Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions.IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria.
31801844	4	115	theme	wild-type	574:582	arg1	strains					602:608	the wild-type and LTA-deficient strains	570:608	the wild-type and LTA-deficient strains of S. gordonii	570:623	Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy.
31801844	11	116	from	homeostasis	2418:2428	arg1	biofilms					2460:2467	biofilms	2460:2467	biofilms	2460:2467	We demonstrate that in S. gordonii, LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells.
31801844	5	117	theme	wall	875:878	arg1	biosynthesis					880:891	cell wall biosynthesis	870:891	cell wall biosynthesis	870:891	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	5	118	dep	DegP	919:922	arg1	iv					915:916	iv	915:916	iv	915:916	Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response).
31801844	2	119	theme	biofilm	329:335	arg1	formation					337:345	biofilm formation	329:345	biofilm formation	329:345	Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division.
31801844	7	120	dep	gordonii	1387:1394	arg1	produced					1441:1448	produced	1441:1448	produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations	1441:1528	We also characterized the chemical structure of the LTA expressed by S. gordonii Similarly to Streptococcus suis, S. gordonii produced a complex type I LTA, decorated with multiple d-alanylations and glycosylations.
32222228	0	0	theme	mass	128:131	arg1	spectrometry					133:144	liquid chromatography-tandem mass spectrometry	99:144	liquid chromatography-tandem mass spectrometry	99:144	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	2	1	theme	ECM	568:570	arg1	Glycosaminoglycans					514:531	Glycosaminoglycans	514:531	Glycosaminoglycans (GAGs)	514:538	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	1	theme	ECM	568:570	arg1	components					554:563	important components	544:563	important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	544:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	3	2	theme	appropriate	919:929	arg1	tools					944:948	appropriate quantitative tools	919:948	appropriate quantitative tools	919:948	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	1	3	theme	biomimetic	291:300	arg1	microenvironment					302:317	a biomimetic microenvironment	289:317	a biomimetic microenvironment	289:317	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	0	4	theme	chromatography-tandem	106:126	arg1	spectrometry					133:144	liquid chromatography-tandem mass spectrometry	99:144	liquid chromatography-tandem mass spectrometry	99:144	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	1	5	theme	availability	396:407	arg1	limitations					381:391	the limitations	377:391	the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	377:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	6	theme	signaling	661:669	arg1	regulators					671:680	signaling regulators	661:680	signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	661:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	7	theme	cell	721:724	arg1	adhesion					726:733	cell adhesion	721:733	cell adhesion	721:733	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	1	8	theme	tissue	223:228	arg1	TE					243:244	TE	243:244	TE	243:244	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	1	8	theme	tissue	223:228	arg1	engineering					230:240	tissue engineering	223:240	tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	223:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	4	9	theme	mass	1125:1128	arg1	LC-MS/MS					1144:1151	LC-MS/MS	1144:1151	LC-MS/MS	1144:1151	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	9	theme	mass	1125:1128	arg1	spectrometry					1130:1141	liquid chromatography-tandem mass spectrometry	1096:1141	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	3	10	theme	ECM	888:890	arg1	composition					860:870	the GAG composition	852:870	the GAG composition of cell-derived ECM	852:890	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	4	11	theme	composition	1267:1277	arg1	terms					1231:1235	terms	1231:1235	terms of their GAG and disaccharide composition	1231:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	1	12	link	tissue-derived	461:474	arg1	scaffolds					503:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	1	13	theme	potential	413:421	arg1	transmission					432:443	potential pathogen transmission	413:443	potential pathogen transmission	413:443	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	3	14	link	cell-derived	875:886	arg1	ECM					888:890	cell-derived ECM	875:890	cell-derived ECM	875:890	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	4	15	theme	decellularized	1177:1190	arg1	ECM					1205:1207	decellularized cell-derived ECM	1177:1207	decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1177:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	1	16	theme	biochemical	329:339	arg1	cues					354:357	biochemical and physical cues	329:357	biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	329:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	1	17	theme	pathogen	423:430	arg1	transmission					432:443	potential pathogen transmission	413:443	potential pathogen transmission	413:443	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	18	theme	processes	702:710	arg1	regulators					671:680	signaling regulators	661:680	signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	661:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	4	19	theme	selective	1086:1094	arg1	method					1154:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	1063:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	20	theme	cell-derived	1192:1203	arg1	ECM					1205:1207	decellularized cell-derived ECM	1177:1207	decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1177:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	1	21	theme	engineering	230:240	arg1	strategies					247:256	tissue engineering (TE) strategies	223:256	tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	223:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	22	theme	crucial	579:585	arg1	role					587:590	a crucial role	577:590	a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	577:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	1	23	theme	transmission	432:443	arg1	limitations					381:391	the limitations	377:391	the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	377:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	24	theme	cellular	693:700	arg1	differentiation					747:761	differentiation	747:761	differentiation	747:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	24	theme	cellular	693:700	arg1	growth					736:741	growth	736:741	growth	736:741	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	24	theme	cellular	693:700	arg1	adhesion					726:733	cell adhesion	721:733	cell adhesion	721:733	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	24	theme	cellular	693:700	arg1	processes					702:710	several cellular processes	685:710	several cellular processes	685:710	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	3	25	theme	quantitative	931:942	arg1	tools					944:948	appropriate quantitative tools	919:948	appropriate quantitative tools	919:948	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	0	26	theme	disaccharide	18:29	arg1	analysis					45:52	Glycosaminoglycan disaccharide compositional analysis	0:52	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices	0:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	2	27	theme	several	685:691	arg1	differentiation					747:761	differentiation	747:761	differentiation	747:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	27	theme	several	685:691	arg1	growth					736:741	growth	736:741	growth	736:741	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	27	theme	several	685:691	arg1	adhesion					726:733	cell adhesion	721:733	cell adhesion	721:733	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	27	theme	several	685:691	arg1	processes					702:710	several cellular processes	685:710	several cellular processes	685:710	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	0	28	theme	liquid	99:104	arg1	spectrometry					133:144	liquid chromatography-tandem mass spectrometry	99:144	liquid chromatography-tandem mass spectrometry	99:144	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	3	29	theme	tools	944:948	arg1	lack					911:914	the lack	907:914	the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples	907:1031	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	0	30	theme	Glycosaminoglycan	0:16	arg1	analysis					45:52	Glycosaminoglycan disaccharide compositional analysis	0:52	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices	0:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	0	31	link	cell-derived	57:68	arg1	matrices					84:91	cell-derived extracellular matrices	57:91	cell-derived extracellular matrices	57:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	1	32	theme	physical	345:352	arg1	cues					354:357	biochemical and physical cues	329:357	biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	329:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	33	theme	mechanical	618:627	arg1	properties					629:638	the mechanical properties	614:638	the mechanical properties of the tissue	614:652	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	1	34	theme	Cell-derived	147:158	arg1	matrices					174:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	35	with	ECM	568:570	arg1	role					587:590	a crucial role	577:590	a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	577:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	3	36	from	subtypes	994:1001	arg1	samples					1025:1031	complex biological samples	1006:1031	complex biological samples	1006:1031	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	0	37	theme	compositional	31:43	arg1	analysis					45:52	Glycosaminoglycan disaccharide compositional analysis	0:52	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices	0:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	1	38	theme	extracellular	160:172	arg1	matrices					174:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	3	39	theme	different	963:971	arg1	GAG					973:975	different GAG and disaccharide subtypes	963:1001	GAG	973:975	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	1	40	theme	due	258:260	arg1	strategies					247:256	tissue engineering (TE) strategies	223:256	tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	223:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	0	41	theme	cell-derived	57:68	arg1	matrices					84:91	cell-derived extracellular matrices	57:91	cell-derived extracellular matrices	57:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	1	42	theme	tissue-derived	461:474	arg1	scaffolds					503:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	2	43	theme	important	544:552	arg1	Glycosaminoglycans					514:531	Glycosaminoglycans	514:531	Glycosaminoglycans (GAGs)	514:538	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	2	43	theme	important	544:552	arg1	components					554:563	important components	544:563	important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation	544:761	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	1	44	theme	extracellular	476:488	arg1	ECM					498:500	ECM	498:500	ECM	498:500	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	1	44	theme	extracellular	476:488	arg1	matrix					490:495	extracellular matrix	476:495	tissue-derived extracellular matrix (ECM) scaffolds	461:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	3	45	from	GAG	973:975	arg1	samples					1025:1031	complex biological samples	1006:1031	complex biological samples	1006:1031	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	3	46	theme	complex	1006:1012	arg1	samples					1025:1031	complex biological samples	1006:1031	complex biological samples	1006:1031	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	0	47	theme	matrices	84:91	arg1	analysis					45:52	Glycosaminoglycan disaccharide compositional analysis	0:52	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices	0:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	2	48	theme	tissue	647:652	arg1	properties					629:638	the mechanical properties	614:638	the mechanical properties of the tissue	614:652	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	1	49	theme	matrix	490:495	arg1	scaffolds					503:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	tissue-derived extracellular matrix (ECM) scaffolds	461:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	3	50	theme	biological	1014:1023	arg1	samples					1025:1031	complex biological samples	1006:1031	complex biological samples	1006:1031	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	3	51	theme	TE	813:814	arg1	field					804:808	the field	800:808	the field of TE	800:814	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	0	52	theme	extracellular	70:82	arg1	matrices					84:91	cell-derived extracellular matrices	57:91	cell-derived extracellular matrices	57:91	Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.
32222228	2	53	from	role	587:590	arg1	maintenance					599:609	the maintenance	595:609	the maintenance of the mechanical properties of the tissue	595:652	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	4	54	theme	disaccharide	1254:1265	arg1	composition					1267:1277	disaccharide composition	1254:1277	disaccharide composition	1254:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	3	55	theme	little	817:822	arg1	information					824:834	little information	817:834	little information	817:834	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	4	56	link	cell-derived	1192:1203	arg1	ECM					1205:1207	decellularized cell-derived ECM	1177:1207	decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1177:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	57	theme	spectrometry	1130:1141	arg1	method					1154:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	1063:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	2	58	theme	properties	629:638	arg1	maintenance					599:609	the maintenance	595:609	the maintenance of the mechanical properties of the tissue	595:652	Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation.
32222228	4	59	theme	GAG	1246:1248	arg1	terms					1231:1235	terms	1231:1235	terms of their GAG and disaccharide composition	1231:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	3	60	theme	cell-derived	875:886	arg1	ECM					888:890	cell-derived ECM	875:890	cell-derived ECM	875:890	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	3	61	theme	disaccharide	981:992	arg1	subtypes					994:1001	different GAG and disaccharide subtypes	963:1001	subtypes	994:1001	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	3	62	theme	GAG	856:858	arg1	composition					860:870	the GAG composition	852:870	the GAG composition of cell-derived ECM	852:890	However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples.
32222228	1	63	link	Cell-derived	147:158	arg1	matrices					174:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices	147:181	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	4	64	theme	chromatography-tandem	1103:1123	arg1	LC-MS/MS					1144:1151	LC-MS/MS	1144:1151	LC-MS/MS	1144:1151	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	64	theme	chromatography-tandem	1103:1123	arg1	spectrometry					1130:1141	liquid chromatography-tandem mass spectrometry	1096:1141	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	1	65	theme	promising	199:207	arg1	scaffolds					209:217	promising scaffolds	199:217	promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds	199:511	Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds.
32222228	4	66	theme	sensitive	1072:1080	arg1	method					1154:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	1063:1159	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	67	theme	liquid	1096:1101	arg1	LC-MS/MS					1144:1151	LC-MS/MS	1144:1151	LC-MS/MS	1144:1151	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
32222228	4	67	theme	liquid	1096:1101	arg1	spectrometry					1130:1141	liquid chromatography-tandem mass spectrometry	1096:1141	a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition	1063:1277	In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.
30799607	4	0	theme	duration	856:863	arg1	time					865:868	a prolonged duration time	844:868	a prolonged duration time	844:868	It was confirmed that Dox and the dye could be simultaneously delivered into HeLa cells or the tumors with a prolonged duration time.
30799607	1	1	dep	acid	273:276	arg1	poly					279:282	poly	279:282	poly(dA)	279:286	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	1	dep	acid	273:276	arg1	dA					284:285	dA	284:285	dA	284:285	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	2	2	from	group	379:383	arg1	t-LNT					401:405	the oxidized t-LNT	388:405	the oxidized t-LNT	388:405	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	1	3	theme	cancer	151:156	arg1	therapy					158:164	cancer therapy	151:164	cancer therapy	151:164	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	4	theme	helix	228:232	arg1	β-glucan					234:241	a natural triple helix β-glucan	211:241	a natural triple helix β-glucan (t-LNT)	211:249	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	4	theme	helix	228:232	arg1	t-LNT					244:248	t-LNT	244:248	t-LNT	244:248	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	2	5	theme	Dox	430:432	arg1	group					421:425	the amino group	411:425	the amino group of Dox	411:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	5	theme	Dox	430:432	arg1	Dox					430:432	Dox	430:432	Dox	430:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	5	theme	Dox	430:432	arg1	group					379:383	the aldehyde group	366:383	the aldehyde group in the oxidized t-LNT	366:405	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	5	theme	Dox	430:432	arg1	chains					446:451	the single chains	435:451	the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA)	435:600	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	6	theme	t-LNT-Dox	294:302	arg1	conjugates					304:313	The t-LNT-Dox conjugates	290:313	The t-LNT-Dox conjugates	290:313	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	1	7	contain	carrying	120:127	arg2	dye					172:174	a dye	170:174	a dye for imaging	170:186	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	7	contain	carrying	120:127	arg2	Dox					142:144	Dox	142:144	Dox	142:144	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	7	contain	carrying	120:127	arg2	doxorubicin					129:139	doxorubicin	129:139	doxorubicin (Dox) for cancer therapy	129:164	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	7	contain	carrying	120:127	arg1	nanoplatform					107:118	A nanoplatform	105:118	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging	105:186	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	3	8	theme	HeLa	708:711	arg1	cells					720:724	HeLa cancer cells	708:724	HeLa cancer cells	708:724	t-LNT-Dox was confirmed to acid-responsively release Dox in vitro, showing enhanced cytotoxicity against HeLa cancer cells with time.
30799607	5	9	theme	free	975:978	arg1	Dox					980:982	the free Dox	971:982	the free Dox	971:982	Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo.
30799607	3	10	theme	cancer	713:718	arg1	cells					720:724	HeLa cancer cells	708:724	HeLa cancer cells	708:724	t-LNT-Dox was confirmed to acid-responsively release Dox in vitro, showing enhanced cytotoxicity against HeLa cancer cells with time.
30799607	5	11	theme	Dox	980:982	arg1	effects					960:966	adverse effects	952:966	adverse effects of the free Dox in vivo	952:990	Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo.
30799607	2	12	theme	dA	542:543	arg1	-dye					545:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	13	theme	s-LNT-Dox/poly	527:540	arg1	-dye					545:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	14	from	group	421:425	arg1	t-LNT					401:405	the oxidized t-LNT	388:405	the oxidized t-LNT	388:405	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	15	theme	composite	517:525	arg1	-dye					545:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	a composite s-LNT-Dox/poly(dA)-dye	515:548	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	16	theme	single	439:444	arg1	group					421:425	the amino group	411:425	the amino group of Dox	411:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	16	theme	single	439:444	arg1	Dox					430:432	Dox	430:432	Dox	430:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	16	theme	single	439:444	arg1	s-LNT-Dox					454:462	s-LNT-Dox	454:462	s-LNT-Dox	454:462	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	16	theme	single	439:444	arg1	chains					446:451	the single chains	435:451	the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA)	435:600	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	6	17	theme	new	1021:1023	arg1	strategy					1025:1032	a new strategy	1019:1032	a new strategy to fabricate the nanoplatform for therapy and imaging using a natural polysaccharide	1019:1117	Hence, this work develops a new strategy to fabricate the nanoplatform for therapy and imaging using a natural polysaccharide.
30799607	2	18	theme	Schiff-base	337:347	arg1	reaction					349:356	Schiff-base reaction	337:356	Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA)	337:600	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	19	theme	oxidized	392:399	arg1	t-LNT					401:405	the oxidized t-LNT	388:405	the oxidized t-LNT	388:405	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	20	theme	hydrogen	558:565	arg1	bonding					567:573	hydrogen bonding	558:573	hydrogen bonding between s-LNT and poly(dA)	558:600	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	6	21	theme	natural	1096:1102	arg1	polysaccharide					1104:1117	a natural polysaccharide	1094:1117	a natural polysaccharide	1094:1117	Hence, this work develops a new strategy to fabricate the nanoplatform for therapy and imaging using a natural polysaccharide.
30799607	1	22	theme	polydeoxyadenylic	255:271	arg1	acid					273:276	polydeoxyadenylic acid	255:276	polydeoxyadenylic acid (poly(dA))	255:287	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	5	23	theme	adverse	952:958	arg1	effects					960:966	adverse effects	952:966	adverse effects of the free Dox in vivo	952:990	Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo.
30799607	4	24	theme	HeLa	814:817	arg1	cells					819:823	HeLa cells	814:823	HeLa cells	814:823	It was confirmed that Dox and the dye could be simultaneously delivered into HeLa cells or the tumors with a prolonged duration time.
30799607	5	25	theme	LNT-Dox	884:890	arg1	conjugates					892:901	LNT-Dox conjugates	884:901	LNT-Dox conjugates	884:901	Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo.
30799607	2	26	theme	aldehyde	370:377	arg1	Dox					430:432	Dox	430:432	Dox	430:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	26	theme	aldehyde	370:377	arg1	group					379:383	the aldehyde group	366:383	the aldehyde group in the oxidized t-LNT	366:405	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	0	27	theme	β-Glucan	32:39	arg1	Acid					63:66	a β-Glucan and Polydeoxyadenylic Acid	30:66	a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging	30:102	Nanoplatform Constructed from a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging.
30799607	4	28	theme	prolonged	846:854	arg1	time					865:868	a prolonged duration time	844:868	a prolonged duration time	844:868	It was confirmed that Dox and the dye could be simultaneously delivered into HeLa cells or the tumors with a prolonged duration time.
30799607	3	29	theme	enhanced	678:685	arg1	cytotoxicity					687:698	enhanced cytotoxicity	678:698	enhanced cytotoxicity against HeLa cancer cells with time	678:734	t-LNT-Dox was confirmed to acid-responsively release Dox in vitro, showing enhanced cytotoxicity against HeLa cancer cells with time.
30799607	2	30	theme	amino	415:419	arg1	Dox					430:432	Dox	430:432	Dox	430:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	30	theme	amino	415:419	arg1	group					421:425	the amino group	411:425	the amino group of Dox	411:432	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	30	theme	amino	415:419	arg1	chains					446:451	the single chains	435:451	the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA)	435:600	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	2	31	theme	dA	499:500	arg1	-dye					502:505	the poly(dA)-dye	490:505	the poly(dA)-dye	490:505	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	0	32	theme	Polydeoxyadenylic	45:61	arg1	Acid					63:66	a β-Glucan and Polydeoxyadenylic Acid	30:66	a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging	30:102	Nanoplatform Constructed from a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging.
30799607	5	33	theme	tumor	925:929	arg1	growth					931:936	tumor growth	925:936	tumor growth	925:936	Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo.
30799607	2	34	theme	poly	494:497	arg1	-dye					502:505	the poly(dA)-dye	490:505	the poly(dA)-dye	490:505	The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA).
30799607	0	35	theme	Cancer	72:77	arg1	Chemotherapy					79:90	Cancer Chemotherapy	72:90	Cancer Chemotherapy	72:90	Nanoplatform Constructed from a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging.
30799607	1	36	theme	natural	213:219	arg1	β-glucan					234:241	a natural triple helix β-glucan	211:241	a natural triple helix β-glucan (t-LNT)	211:249	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	36	theme	natural	213:219	arg1	t-LNT					244:248	t-LNT	244:248	t-LNT	244:248	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	0	37	dep	Nanoplatform	0:11	arg1	Constructed					13:23	Constructed	13:23	Nanoplatform Constructed from a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging	0:102	Nanoplatform Constructed from a β-Glucan and Polydeoxyadenylic Acid for Cancer Chemotherapy and Imaging.
30799607	3	38	with	cytotoxicity	687:698	arg1	time					731:734	time	731:734	time	731:734	t-LNT-Dox was confirmed to acid-responsively release Dox in vitro, showing enhanced cytotoxicity against HeLa cancer cells with time.
30799607	1	39	theme	triple	221:226	arg1	β-glucan					234:241	a natural triple helix β-glucan	211:241	a natural triple helix β-glucan (t-LNT)	211:249	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30799607	1	39	theme	triple	221:226	arg1	t-LNT					244:248	t-LNT	244:248	t-LNT	244:248	A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)).
30280699	2	0	theme	collagen	350:357	arg1	Type					359:362	collagen Type I	350:364	collagen Type I hydrogel/SF	350:376	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	9	1	theme	SF	1389:1390	arg1	groups					1392:1397	the CH/SF and SF groups	1375:1397	groups	1392:1397	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	4	2	theme	Histological	648:659	arg1	analysis					661:668	Histological analysis	648:668	Histological analysis of the incubated scaffolds	648:695	Histological analysis of the incubated scaffolds was performed to evaluate the neovascularization capacity, and the outcomes of urethroplasty were evaluated at one and three months post-operatively.
30280699	11	3	theme	significant	1606:1616	arg1	increase					1618:1625	a significant increase	1604:1625	a significant increase at each time point (p < 0.05)	1604:1655	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	11	4	theme	muscle	1529:1534	arg1	content					1536:1542	smooth muscle content	1522:1542	smooth muscle content	1522:1542	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	1	5	theme	regeneration	184:195	arg1	effects					164:170	the effects	160:170	the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	160:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	1	6	theme	composite	277:285	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	1	7	from	scaffolds	287:295	arg1	model					309:313	a rabbit model	300:313	a rabbit model	300:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	3	8	theme	male	559:562	arg1	rabbits					564:570	male rabbits	559:570	male rabbits	559:570	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	1	9	theme	prevascularized	202:216	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	6	10	theme	histological	966:977	arg1	analysis					979:986	The histological analysis	962:986	The histological analysis of the incubated BAMH/SF	962:1011	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	3	11	theme	CH/SF	503:507	arg1	scaffolds					516:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	1	12	theme	acellular	226:234	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	1	13	theme	hydrogel	243:250	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	6	14	theme	signifcant	1024:1033	arg1	increase					1035:1042	a signifcant increase	1022:1042	a signifcant increase of the neovascularization	1022:1068	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	4	15	theme	incubated	677:685	arg1	scaffolds					687:695	the incubated scaffolds	673:695	the incubated scaffolds	673:695	Histological analysis of the incubated scaffolds was performed to evaluate the neovascularization capacity, and the outcomes of urethroplasty were evaluated at one and three months post-operatively.
30280699	6	16	theme	incubated	995:1003	arg1	BAMH/SF					1005:1011	the incubated BAMH/SF	991:1011	the incubated BAMH/SF	991:1011	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	9	17	located	found	1366:1370	arg2	epithelium					1351:1360	one layer epithelium	1341:1360	one layer epithelium	1341:1360	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	9	17	located	found	1366:1370	arg1	groups					1392:1397	the CH/SF and SF groups	1375:1397	groups	1392:1397	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	0	18	theme	urethra	111:117	arg1	regeneration					95:106	the regeneration	91:106	the regeneration of urethra	91:117	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	4	19	theme	urethroplasty	776:788	arg1	outcomes					764:771	the outcomes	760:771	the outcomes of urethroplasty	760:788	Histological analysis of the incubated scaffolds was performed to evaluate the neovascularization capacity, and the outcomes of urethroplasty were evaluated at one and three months post-operatively.
30280699	0	20	theme	fibroin	55:61	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	2	21	theme	scaffolds	435:443	arg1	structure					418:426	the structure	414:426	the structure of the scaffolds	414:443	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	10	22	theme	epithelial	1427:1436	arg1	regeneration					1438:1449	similar epithelial regeneration	1419:1449	similar epithelial regeneration	1419:1449	However, there was similar epithelial regeneration in BAMH/SF and CH/SF groups at three months (p > 0.05).
30280699	0	23	theme	Prevascularized	0:14	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	9	24	theme	BAMH/SF	1322:1328	arg1	group					1330:1334	the BAMH/SF group	1318:1334	the BAMH/SF group	1318:1334	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	5	25	theme	SF	946:947	arg1	layer					955:959	compact SF outer layer	938:959	compact SF outer layer	938:959	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	0	26	theme	acellular	24:32	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	12	27	theme	muscle	1835:1840	arg1	regeneration					1784:1795	the regeneration	1780:1795	the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction	1780:1899	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	6	28	theme	neovascularization	1051:1068	arg1	increase					1035:1042	a signifcant increase	1022:1042	a signifcant increase of the neovascularization	1022:1068	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	2	29	theme	scanning	464:471	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy	464:491	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	0	30	theme	hydrogel/silk	41:53	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	1	31	theme	rabbit	302:307	arg1	model					309:313	a rabbit model	300:313	a rabbit model	300:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	3	32	theme	autologous	619:628	arg1	defects					639:645	autologous urethral defects	619:645	autologous urethral defects	619:645	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	0	33	theme	composite	63:71	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	12	34	theme	urethral	1804:1811	arg1	epithelium					1813:1822	urethral epithelium	1804:1822	urethral epithelium	1804:1822	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	11	35	theme	vessel	1548:1553	arg1	density					1555:1561	vessel density	1548:1561	vessel density	1548:1561	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	2	36	theme	METHODS	330:336	arg1	BAMH/SF					338:344	MATERIALS AND METHODS BAMH/SF	316:344	MATERIALS AND METHODS BAMH/SF	316:344	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	2	36	theme	METHODS	330:336	arg1	CH/SF					379:383	CH/SF	379:383	CH/SF	379:383	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	12	37	theme	prevascularized	1748:1762	arg1	BAMH/SF					1764:1770	prevascularized BAMH/SF	1748:1770	prevascularized BAMH/SF	1748:1770	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	2	38	theme	hydrogel/SF	366:376	arg1	scaffolds					386:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds	316:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds	316:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	12	39	dep	epithelium	1813:1822	arg1	the					1800:1802	the	1800:1802	the	1800:1802	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	2	40	theme	Type	359:362	arg1	hydrogel/SF					366:376	collagen Type I hydrogel/SF	350:376	collagen Type I hydrogel/SF	350:376	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	1	41	theme	fibroin	264:270	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	1	42	theme	/silk	258:262	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	6	43	theme	two-week	1097:1104	arg1	incubation					1106:1115	a two-week incubation	1095:1115	a two-week incubation	1095:1115	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	12	44	dep	CONCLUSION	1658:1667	arg1	demonstrate					1681:1691	demonstrate	1681:1691	demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction	1681:1899	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	1	45	theme	SF	273:274	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	1	46	theme	urethral	175:182	arg1	regeneration					184:195	urethral regeneration	175:195	urethral regeneration	175:195	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	8	47	theme	SF	1232:1233	arg1	groups					1235:1240	the CH/SF and SF groups	1218:1240	the CH/SF and SF groups	1218:1240	Strictures were found in the CH/SF and SF groups.
30280699	11	48	theme	content	1536:1542	arg1	Comparisons					1507:1517	Comparisons	1507:1517	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF	1507:1593	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	2	49	theme	MATERIALS	316:324	arg1	BAMH/SF					338:344	MATERIALS AND METHODS BAMH/SF	316:344	MATERIALS AND METHODS BAMH/SF	316:344	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	2	49	theme	MATERIALS	316:324	arg1	CH/SF					379:383	CH/SF	379:383	CH/SF	379:383	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	12	50	theme	incubated	1698:1706	arg1	BAMH/SF					1708:1714	incubated BAMH/SF	1698:1714	incubated BAMH/SF	1698:1714	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	11	51	theme	smooth	1522:1527	arg1	content					1536:1542	smooth muscle content	1522:1542	smooth muscle content	1522:1542	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	1	52	theme	bladder	218:224	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	2	53	theme	BAMH/SF	338:344	arg1	scaffolds					386:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds	316:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds	316:394	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	1	54	theme	matrix	236:241	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	3	55	theme	rabbits	564:570	arg1	omentum					548:554	the omentum	544:554	the omentum of male rabbits	544:570	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	8	56	theme	CH/SF	1222:1226	arg1	groups					1235:1240	the CH/SF and SF groups	1218:1240	the CH/SF and SF groups	1218:1240	Strictures were found in the CH/SF and SF groups.
30280699	1	57	with	effects	164:170	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	5	58	theme	porous	906:911	arg1	BAMH					913:916	BAMH	913:916	BAMH	913:916	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	6	59	theme	BAMH/SF	1005:1011	arg1	analysis					979:986	The histological analysis	962:986	The histological analysis of the incubated BAMH/SF	962:1011	The histological analysis of the incubated BAMH/SF revealed a signifcant increase of the neovascularization among three groups after a two-week incubation.
30280699	2	60	theme	electron	473:480	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy	464:491	MATERIALS AND METHODS BAMH/SF and collagen Type I hydrogel/SF (CH/SF) scaffolds were prepared and the structure of the scaffolds was assessed using scanning electron microscopy.
30280699	4	61	theme	scaffolds	687:695	arg1	analysis					661:668	Histological analysis	648:668	Histological analysis of the incubated scaffolds	648:695	Histological analysis of the incubated scaffolds was performed to evaluate the neovascularization capacity, and the outcomes of urethroplasty were evaluated at one and three months post-operatively.
30280699	9	62	theme	multilayer	1284:1293	arg1	epithelium					1295:1304	multilayer epithelium	1284:1304	multilayer epithelium	1284:1304	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	11	63	from	point	1640:1644	arg1	increase					1618:1625	a significant increase	1604:1625	a significant increase at each time point (p < 0.05)	1604:1655	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	0	64	theme	rabbit	124:129	arg1	model					131:135	a rabbit model	122:135	a rabbit model	122:135	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	0	65	theme	bladder	16:22	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	12	66	theme	urethral	1877:1884	arg1	reconstruction					1886:1899	urethral reconstruction	1877:1899	urethral reconstruction	1877:1899	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	5	67	theme	compact	938:944	arg1	layer					955:959	compact SF outer layer	938:959	compact SF outer layer	938:959	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	10	68	theme	similar	1419:1425	arg1	regeneration					1438:1449	similar epithelial regeneration	1419:1449	similar epithelial regeneration	1419:1449	However, there was similar epithelial regeneration in BAMH/SF and CH/SF groups at three months (p > 0.05).
30280699	4	69	theme	neovascularization	727:744	arg1	capacity					746:753	the neovascularization capacity	723:753	the neovascularization capacity	723:753	Histological analysis of the incubated scaffolds was performed to evaluate the neovascularization capacity, and the outcomes of urethroplasty were evaluated at one and three months post-operatively.
30280699	0	70	theme	matrix	34:39	arg1	scaffolds					73:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds	0:81	Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model.
30280699	5	71	theme	outer	949:953	arg1	layer					955:959	compact SF outer layer	938:959	compact SF outer layer	938:959	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	10	72	theme	CH/SF	1466:1470	arg1	groups					1472:1477	BAMH/SF and CH/SF groups	1454:1477	BAMH/SF and CH/SF groups	1454:1477	However, there was similar epithelial regeneration in BAMH/SF and CH/SF groups at three months (p > 0.05).
30280699	3	73	theme	urethral	630:637	arg1	defects					639:645	autologous urethral defects	619:645	autologous urethral defects	619:645	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	7	74	theme	wide	1158:1161	arg1	caliber					1163:1169	wide caliber	1158:1169	wide caliber	1158:1169	At three months, the urethra maintained wide caliber in the BAMH/SF group.
30280699	10	75	theme	BAMH/SF	1454:1460	arg1	groups					1472:1477	BAMH/SF and CH/SF groups	1454:1477	BAMH/SF and CH/SF groups	1454:1477	However, there was similar epithelial regeneration in BAMH/SF and CH/SF groups at three months (p > 0.05).
30280699	11	76	theme	time	1635:1638	arg1	p < 0.05					1647:1654	p < 0.05	1647:1654	p < 0.05	1647:1654	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	11	76	theme	time	1635:1638	arg1	point					1640:1644	each time point	1630:1644	each time point (p < 0.05)	1630:1655	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	11	77	theme	density	1555:1561	arg1	Comparisons					1507:1517	Comparisons	1507:1517	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF	1507:1593	Comparisons of smooth muscle content and vessel density among the SF, CH/SF and BAMH/SF revealed a significant increase at each time point (p < 0.05).
30280699	9	78	theme	layer	1345:1349	arg1	epithelium					1351:1360	one layer epithelium	1341:1360	one layer epithelium	1341:1360	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30280699	12	79	theme	epithelium	1813:1822	arg1	regeneration					1784:1795	the regeneration	1780:1795	the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction	1780:1899	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	8	80	located	found	1209:1213	arg1	groups					1235:1240	the CH/SF and SF groups	1218:1240	the CH/SF and SF groups	1218:1240	Strictures were found in the CH/SF and SF groups.
30280699	8	80	located	found	1209:1213	arg2	Strictures					1193:1202	Strictures	1193:1202	Strictures	1193:1202	Strictures were found in the CH/SF and SF groups.
30280699	3	81	theme	BAMH/SF	494:500	arg1	scaffolds					516:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	12	82	theme	smooth	1828:1833	arg1	muscle					1835:1840	smooth muscle	1828:1840	smooth muscle	1828:1840	CONCLUSION Our results demonstrate that incubated BAMH/SF promote neovascularization, and prevascularized BAMH/SF promote the regeneration of the urethral epithelium and smooth muscle, which indicates its potential for urethral reconstruction.
30280699	3	83	theme	SF	513:514	arg1	scaffolds					516:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds	494:524	BAMH/SF, CH/SF and SF scaffolds were incubated in the omentum of male rabbits for two weeks and then harvested for repairing autologous urethral defects.
30280699	1	84	theme	BAMH	253:256	arg1	scaffolds					287:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds	202:295	prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model	202:313	OBJECTIVE To evaluate the effects of urethral regeneration with prevascularized bladder acellular matrix hydrogel (BAMH)/silk fibroin (SF) composite scaffolds in a rabbit model.
30280699	5	85	theme	composited	859:868	arg1	scaffolds					870:878	The composited scaffolds	855:878	The composited scaffolds	855:878	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	5	86	dep	RESULTS	847:853	arg1	composed					885:892	composed	885:892	were composed of a highly porous BAMH or CH buttressed by compact SF outer layer	880:959	RESULTS The composited scaffolds were composed of a highly porous BAMH or CH buttressed by compact SF outer layer.
30280699	7	87	theme	BAMH/SF	1178:1184	arg1	group					1186:1190	the BAMH/SF group	1174:1190	the BAMH/SF group	1174:1190	At three months, the urethra maintained wide caliber in the BAMH/SF group.
30280699	9	88	theme	CH/SF	1379:1383	arg1	groups					1392:1397	the CH/SF and SF groups	1375:1397	groups	1392:1397	Histologically, at one month, intact and multilayer epithelium occurred in the BAMH/SF group, and one layer epithelium was found in the CH/SF and SF groups.
30840866	1	0	theme	microfibrillated	182:197	arg1	chitin					199:204	the nano-sized microfibrillated chitin	167:204	the nano-sized microfibrillated chitin	167:204	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	4	1	theme	elastic	572:578	arg1	modulus					580:586	the elastic modulus	568:586	the elastic modulus	568:586	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	6	2	theme	oxygen	902:907	arg1	resistance					909:918	the oxygen resistance	898:918	the oxygen resistance of composite film	898:936	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	4	3	theme	film	654:657	arg1	modulus					580:586	the elastic modulus	568:586	the elastic modulus	568:586	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	4	3	theme	film	654:657	arg1	strength					600:607	tensile strength	592:607	tensile strength	592:607	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	5	4	theme	6 wt	802:805	arg1	content					808:814	the 6 wt% content	798:814	the 6 wt% content of microfibrillated chitin	798:841	The swelling ratio decreased to 11.63 with the 6 wt% content of microfibrillated chitin.
30840866	7	5	theme	gelatin	1027:1033	arg1	performance					1035:1045	gelatin performance	1027:1045	gelatin performance	1027:1045	This work expects to provide a pathway to improve gelatin performance.
30840866	1	6	theme	Microfibrillated	74:89	arg1	phase					219:223	reinforce phase	209:223	reinforce phase	209:223	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	1	6	theme	Microfibrillated	74:89	arg1	films					116:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	4	7	theme	8 wt	748:751	arg1	content					710:716	the content	706:716	the content of microfibrillated chitin	706:743	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	4	7	theme	8 wt	748:751	arg1	%					752:752	8 wt%	748:752	8 wt%	748:752	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	5	8	theme	%	806:806	arg1	content					808:814	the 6 wt% content	798:814	the 6 wt% content of microfibrillated chitin	798:841	The swelling ratio decreased to 11.63 with the 6 wt% content of microfibrillated chitin.
30840866	1	9	theme	chitin/gelatin	91:104	arg1	phase					219:223	reinforce phase	209:223	reinforce phase	209:223	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	1	9	theme	chitin/gelatin	91:104	arg1	films					116:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	1	10	theme	composite	106:114	arg1	phase					219:223	reinforce phase	209:223	reinforce phase	209:223	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	1	10	theme	composite	106:114	arg1	films					116:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films	74:120	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	2	11	theme	texture	435:441	arg1	analyzer					443:450	texture analyzer	435:450	texture analyzer	435:450	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30840866	3	12	theme	Oxygen	453:458	arg1	property					473:480	Oxygen permeability property	453:480	Oxygen permeability property	453:480	Oxygen permeability property, optical property and swelling property were investigated.
30840866	6	13	theme	transmittance	962:974	arg1	loss					954:957	obvious loss	946:957	obvious loss of transmittance	946:974	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	6	14	theme	chitin	857:862	arg1	microfibrils					864:875	chitin microfibrils	857:875	chitin microfibrils	857:875	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	4	15	theme	microfibrillated	612:627	arg1	film					654:657	microfibrillated chitin/gelatin composite film	612:657	microfibrillated chitin/gelatin composite film	612:657	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	2	16	theme	electron	357:364	arg1	SEM					378:380	SEM	378:380	SEM	378:380	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30840866	2	16	theme	electron	357:364	arg1	microscope					366:375	scanning electron microscope	348:375	scanning electron microscope (SEM)	348:381	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30840866	2	17	theme	scanning	348:355	arg1	SEM					378:380	SEM	378:380	SEM	378:380	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30840866	2	17	theme	scanning	348:355	arg1	microscope					366:375	scanning electron microscope	348:375	scanning electron microscope (SEM)	348:381	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30840866	4	18	theme	composite	644:652	arg1	film					654:657	microfibrillated chitin/gelatin composite film	612:657	microfibrillated chitin/gelatin composite film	612:657	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	1	19	theme	solvent	139:145	arg1	method					155:160	solvent casting method	139:160	solvent casting method	139:160	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	1	20	theme	oxygen	236:241	arg1	resistance					243:252	oxygen resistance	236:252	oxygen resistance	236:252	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	0	21	theme	chitin/gelatin	47:60	arg1	composites					62:71	microfibrillated chitin/gelatin composites	30:71	microfibrillated chitin/gelatin composites	30:71	Preparation and properties of microfibrillated chitin/gelatin composites.
30840866	4	22	theme	chitin/gelatin	629:642	arg1	film					654:657	microfibrillated chitin/gelatin composite film	612:657	microfibrillated chitin/gelatin composite film	612:657	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	1	23	dep	phase	219:223	arg1	reinforce					209:217	reinforce	209:217	reinforce	209:217	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	4	24	theme	chitin	738:743	arg1	content					710:716	the content	706:716	the content of microfibrillated chitin	706:743	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	4	24	theme	chitin	738:743	arg1	%					752:752	8 wt%	748:752	8 wt%	748:752	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	0	25	theme	microfibrillated	30:45	arg1	composites					62:71	microfibrillated chitin/gelatin composites	30:71	microfibrillated chitin/gelatin composites	30:71	Preparation and properties of microfibrillated chitin/gelatin composites.
30840866	3	26	theme	permeability	460:471	arg1	property					473:480	Oxygen permeability property	453:480	Oxygen permeability property	453:480	Oxygen permeability property, optical property and swelling property were investigated.
30840866	6	27	theme	obvious	946:952	arg1	loss					954:957	obvious loss	946:957	obvious loss of transmittance	946:974	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	3	28	theme	swelling	504:511	arg1	property					513:520	swelling property	504:520	swelling property	504:520	Oxygen permeability property, optical property and swelling property were investigated.
30840866	3	29	theme	optical	483:489	arg1	property					491:498	optical property	483:498	optical property	483:498	Oxygen permeability property, optical property and swelling property were investigated.
30840866	1	30	theme	casting	147:153	arg1	method					155:160	solvent casting method	139:160	solvent casting method	139:160	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	0	31	theme	composites	62:71	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of microfibrillated chitin/gelatin composites.
30840866	0	31	theme	composites	62:71	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of microfibrillated chitin/gelatin composites.
30840866	5	32	theme	swelling	759:766	arg1	ratio					768:772	The swelling ratio	755:772	The swelling ratio	755:772	The swelling ratio decreased to 11.63 with the 6 wt% content of microfibrillated chitin.
30840866	6	33	theme	film	933:936	arg1	resistance					909:918	the oxygen resistance	898:918	the oxygen resistance of composite film	898:936	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	4	34	theme	tensile	592:598	arg1	strength					600:607	tensile strength	592:607	tensile strength	592:607	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	6	35	theme	composite	923:931	arg1	film					933:936	composite film	923:936	composite film	923:936	In addition, chitin microfibrils effectively enhanced the oxygen resistance of composite film without obvious loss of transmittance.
30840866	1	36	theme	nano-sized	171:180	arg1	chitin					199:204	the nano-sized microfibrillated chitin	167:204	the nano-sized microfibrillated chitin	167:204	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	5	37	theme	microfibrillated	819:834	arg1	chitin					836:841	microfibrillated chitin	819:841	microfibrillated chitin	819:841	The swelling ratio decreased to 11.63 with the 6 wt% content of microfibrillated chitin.
30840866	4	38	theme	microfibrillated	721:736	arg1	chitin					738:743	microfibrillated chitin	721:743	microfibrillated chitin	721:743	The results indicated that the elastic modulus and tensile strength of microfibrillated chitin/gelatin composite film reached 2.2 GPa and 74.5 MPa respectively when the content of microfibrillated chitin is 8 wt%.
30840866	1	39	theme	mechanical	275:284	arg1	performance					286:296	mechanical performance	275:296	mechanical performance	275:296	Microfibrillated chitin/gelatin composite films were prepared by solvent casting method, and the nano-sized microfibrillated chitin as reinforce phase to improve oxygen resistance, water-resistant and mechanical performance in this system.
30840866	5	40	theme	chitin	836:841	arg1	content					808:814	the 6 wt% content	798:814	the 6 wt% content of microfibrillated chitin	798:841	The swelling ratio decreased to 11.63 with the 6 wt% content of microfibrillated chitin.
30840866	2	41	theme	mechanical	392:401	arg1	properties					403:412	the mechanical properties	388:412	the mechanical properties	388:412	The morphologies were analyzed by scanning electron microscope (SEM), and the mechanical properties were investigated by texture analyzer.
30755510	8	0	theme	Biochemical	1098:1108	arg1	analyses					1110:1117	Biochemical analyses	1098:1117	Biochemical analyses	1098:1117	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	15	1	theme	biosynthetic	2233:2244	arg1	pathway					2246:2252	biosynthetic pathway	2233:2252	biosynthetic pathway	2233:2252	Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined.
30755510	16	2	theme	wall	2389:2392	arg1	galactomannan					2394:2406	the cell wall galactomannan	2380:2406	the cell wall galactomannan	2380:2406	Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan.
30755510	17	3	theme	polarization	2470:2481	arg1	misregulation					2453:2465	misregulation	2453:2465	misregulation of polarization and altered conidiation	2453:2505	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	2	4	theme	biosynthetic	284:295	arg1	pathway					297:303	their biosynthetic pathway	278:303	their biosynthetic pathway	278:303	In contrast to yeasts, their biosynthetic pathway has been poorly investigated in filamentous fungi.
30755510	7	5	theme	α-1,2-mannosyltransferase	1037:1061	arg1	activity					1063:1070	α-1,2-mannosyltransferase activity	1037:1070	α-1,2-mannosyltransferase activity	1037:1070	In particular, we describe a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene.
30755510	1	6	theme	complex	171:177	arg1	polysaccharides					192:206	complex carbohydrate polysaccharides	171:206	complex carbohydrate polysaccharides with different structures in yeasts and molds	171:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	6	theme	complex	171:177	arg1	mannans					159:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	12	7	theme	essential	1886:1894	arg1	wall					1849:1852	The fungal cell wall	1833:1852	The fungal cell wall	1833:1852	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	7	theme	essential	1886:1894	arg1	entity					1879:1884	a complex and dynamic entity	1857:1884	a complex and dynamic entity essential for the development of fungi	1857:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	9	8	from	virulence	1429:1437	arg1	model					1450:1454	a mouse model	1442:1454	a mouse model of invasive aspergillosis	1442:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	14	9	theme	cell	2080:2083	arg1	wall					2085:2088	The cell wall	2076:2088	The cell wall	2076:2088	The cell wall is mainly composed of polysaccharides organized in a three-dimensional network.
30755510	8	10	theme	gene	1152:1155	arg1	deletion					1131:1138	deletion	1131:1138	deletion of the KTR4 gene or the KTR7 gene	1131:1172	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	8	11	theme	cell	1198:1201	arg1	galactomannan					1208:1220	cell wall galactomannan	1198:1220	cell wall galactomannan	1198:1220	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	10	12	theme	KTR	1497:1499	arg1	proteins					1501:1508	the KTR proteins	1493:1508	the KTR proteins	1493:1508	In yeast, the KTR proteins are involved in protein 0- and N-glycosylation.
30755510	4	13	with	mannan	529:534	arg1	unit					553:556	a repeating unit	541:556	a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	541:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	1	14	theme	different	213:221	arg1	structures					223:232	different structures	213:232	different structures in yeasts and molds	213:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	12	15	theme	complex	1859:1865	arg1	wall					1849:1852	The fungal cell wall	1833:1852	The fungal cell wall	1833:1852	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	15	theme	complex	1859:1865	arg1	entity					1879:1884	a complex and dynamic entity	1857:1884	a complex and dynamic entity essential for the development of fungi	1857:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	17	16	theme	strain	2590:2595	arg1	conidia					2566:2572	the conidia	2562:2572	the conidia of the parental strain	2562:2595	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	8	17	theme	galactomannan	1208:1220	arg1	absence					1187:1193	the absence	1183:1193	the absence of cell wall galactomannan	1183:1220	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	1	18	from	structures	223:232	arg1	molds					248:252	molds	248:252	molds	248:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	18	from	structures	223:232	arg1	yeasts					237:242	yeasts	237:242	yeasts	237:242	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	9	19	theme	viability	1406:1414	arg1	virulence					1429:1437	reduced virulence	1421:1437	reduced virulence in a mouse model of invasive aspergillosis	1421:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	19	theme	viability	1406:1414	arg1	conidiation					1359:1369	conidiation	1359:1369	conidiation	1359:1369	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	19	theme	viability	1406:1414	arg1	alteration					1379:1388	marked alteration	1372:1388	marked alteration of the conidial viability	1372:1414	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	8	20	theme	gene	1169:1172	arg1	deletion					1131:1138	deletion	1131:1138	deletion of the KTR4 gene or the KTR7 gene	1131:1172	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	11	21	theme	biological	1669:1678	arg1	functions					1680:1688	very different biological functions	1654:1688	very different biological functions	1654:1688	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	11	22	contain	have	1649:1652	arg1	proteins					1635:1642	proteins	1635:1642	proteins that have very different biological functions in yeasts and filamentous fungi	1635:1720	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	11	22	contain	have	1649:1652	arg2	functions					1680:1688	very different biological functions	1654:1688	very different biological functions	1654:1688	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	18	23	theme	cell	2720:2723	arg1	stability					2730:2738	cell wall stability	2720:2738	cell wall stability	2720:2738	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	15	24	theme	wall	2208:2211	arg1	galactomannan					2213:2225	a cell wall galactomannan	2201:2225	a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined	2201:2299	Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined.
30755510	1	25	theme	cell	149:152	arg1	polysaccharides					192:206	complex carbohydrate polysaccharides	171:206	complex carbohydrate polysaccharides with different structures in yeasts and molds	171:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	25	theme	cell	149:152	arg1	mannans					159:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	0	26	theme	Wall	74:77	arg1	Biosynthesis					53:64	the Biosynthesis	49:64	the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus	49:139	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	3	27	from	galactomannan	414:426	arg1	fumigatus					371:379	Aspergillus fumigatus	359:379	Aspergillus fumigatus	359:379	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	12	28	theme	fungal	1837:1842	arg1	wall					1849:1852	The fungal cell wall	1833:1852	The fungal cell wall	1833:1852	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	28	theme	fungal	1837:1842	arg1	entity					1879:1884	a complex and dynamic entity	1857:1884	a complex and dynamic entity essential for the development of fungi	1857:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	17	29	theme	altered	2487:2493	arg1	conidiation					2495:2505	altered conidiation	2487:2505	altered conidiation	2487:2505	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	18	30	theme	low	2637:2639	arg1	concentration					2641:2653	its low concentration	2633:2653	its low concentration in the cell wall	2633:2670	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	9	31	theme	parental	1240:1247	arg1	strains					1249:1255	parental strains	1240:1255	parental strains	1240:1255	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	32	theme	invasive	1459:1466	arg1	aspergillosis					1468:1480	invasive aspergillosis	1459:1480	invasive aspergillosis	1459:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	6	33	theme	them	926:929	arg1	them					926:929	them	926:929	them	926:929	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	6	33	theme	them	926:929	arg1	two					919:921	two	919:921	two	919:921	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	0	34	theme	Polarized	99:107	arg1	Growth					109:114	Polarized Growth	99:114	Polarized Growth	99:114	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	13	35	theme	environmental	1964:1976	arg1	challenge					1978:1986	environmental challenge	1964:1986	environmental challenge posed by nutrient stress and host defenses	1964:2029	It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth.
30755510	3	36	theme	mannan	392:397	arg1	structure					399:407	the major mannan structure	382:407	the major mannan structure	382:407	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	3	36	theme	mannan	392:397	arg1	galactomannan					414:426	a galactomannan	412:426	a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core	412:489	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	9	37	theme	Δktr4	1262:1266	arg1	mutants					1278:1284	the Δktr4 and Δktr7 mutants	1258:1284	mutants	1278:1284	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	0	38	dep	Wall	74:77	arg1	Mannans					79:85	Mannans	79:85	Mannans	79:85	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	0	38	dep	Wall	74:77	arg1	Growth					109:114	Polarized Growth	99:114	Polarized Growth	99:114	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	3	39	theme	wall	481:484	arg1	core					486:489	the β-1,3-glucan-chitin cell wall core	452:489	the β-1,3-glucan-chitin cell wall core	452:489	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	9	40	theme	Δktr7	1272:1276	arg1	mutants					1278:1284	the Δktr4 and Δktr7 mutants	1258:1284	mutants	1278:1284	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	6	41	theme	family	859:864	arg1	members					817:823	three members	811:823	three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7)	811:887	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	14	42	theme	three-dimensional	2143:2159	arg1	network					2161:2167	a three-dimensional network	2141:2167	a three-dimensional network	2141:2167	The cell wall is mainly composed of polysaccharides organized in a three-dimensional network.
30755510	3	43	theme	β-1,3-glucan-chitin	456:474	arg1	core					486:489	the β-1,3-glucan-chitin cell wall core	452:489	the β-1,3-glucan-chitin cell wall core	452:489	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	6	44	dep	named	842:846	arg1	also					837:840	also	837:840	also	837:840	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	18	45	theme	wall	2725:2728	arg1	stability					2730:2738	cell wall stability	2720:2738	cell wall stability	2720:2738	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	9	46	theme	severe	1295:1300	arg1	phenotype					1309:1317	a severe growth phenotype	1293:1317	a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis	1293:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	12	47	theme	cell	1750:1753	arg1	mannans					1760:1766	cell wall mannans	1750:1766	cell wall mannans	1750:1766	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	48	from	fumigatus	1739:1747	arg1	important					1775:1783	important	1775:1783	important	1775:1783	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	9	49	dep	strains	1249:1255	arg1	comparison					1226:1235	comparison	1226:1235	comparison	1226:1235	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	2	50	theme	filamentous	337:347	arg1	fungi					349:353	filamentous fungi	337:353	filamentous fungi	337:353	In contrast to yeasts, their biosynthetic pathway has been poorly investigated in filamentous fungi.
30755510	15	51	theme	biological	2258:2267	arg1	functions					2269:2277	biological functions	2258:2277	biological functions	2258:2277	Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined.
30755510	6	52	theme	Ktr	832:834	arg1	family					859:864	the Ktr (also named Kre2/Mnt1) family	828:864	the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7)	828:887	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	17	53	theme	growth	2434:2439	arg1	defect					2441:2446	a growth defect	2432:2446	a growth defect with misregulation of polarization and altered conidiation	2432:2505	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	18	54	theme	apical	2745:2750	arg1	growth					2752:2757	apical growth	2745:2757	apical growth	2745:2757	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	16	55	theme	cell	2384:2387	arg1	galactomannan					2394:2406	the cell wall galactomannan	2380:2406	the cell wall galactomannan	2380:2406	Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan.
30755510	9	56	from	conidiation	1359:1369	arg1	model					1450:1454	a mouse model	1442:1454	a mouse model of invasive aspergillosis	1442:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	10	57	dep	protein	1526:1532	arg1	N-glycosylation					1541:1555	N-glycosylation	1541:1555	N-glycosylation	1541:1555	In yeast, the KTR proteins are involved in protein 0- and N-glycosylation.
30755510	10	57	dep	protein	1526:1532	arg1	0-					1534:1535	0-	1534:1535	0-	1534:1535	In yeast, the KTR proteins are involved in protein 0- and N-glycosylation.
30755510	12	58	theme	wall	1755:1758	arg1	mannans					1760:1766	cell wall mannans	1750:1766	cell wall mannans	1750:1766	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	9	59	theme	polarized	1335:1343	arg1	growth					1345:1350	polarized growth	1335:1350	polarized growth	1335:1350	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	16	60	theme	galactomannan	2394:2406	arg1	synthesis					2367:2375	the synthesis	2363:2375	the synthesis of the cell wall galactomannan	2363:2406	Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan.
30755510	4	61	theme	α1,6-linked	575:585	arg1	mannoses					603:610	four α1,6-linked and α1,2-linked mannoses	570:610	four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	570:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	7	62	theme	activity	1063:1070	arg1	form					1029:1032	a newly discovered form	1010:1032	a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene	1010:1095	In particular, we describe a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene.
30755510	6	63	theme	members	817:823	arg1	function					799:806	the function	795:806	the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus	795:903	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	13	64	theme	polarized	2058:2066	arg1	growth					2068:2073	polarized growth	2058:2073	polarized growth	2058:2073	It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth.
30755510	9	65	theme	mouse	1444:1448	arg1	model					1450:1454	a mouse model	1442:1454	a mouse model of invasive aspergillosis	1442:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	4	66	theme	α1,2-linked	591:601	arg1	mannoses					603:610	four α1,6-linked and α1,2-linked mannoses	570:610	four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	570:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	17	67	with	defect	2441:2446	arg1	misregulation					2453:2465	misregulation	2453:2465	misregulation of polarization and altered conidiation	2453:2505	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	18	68	theme	cell	2662:2665	arg1	wall					2667:2670	the cell wall	2658:2670	the cell wall	2658:2670	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	1	69	theme	carbohydrate	179:190	arg1	polysaccharides					192:206	complex carbohydrate polysaccharides	171:206	complex carbohydrate polysaccharides with different structures in yeasts and molds	171:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	69	theme	carbohydrate	179:190	arg1	mannans					159:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	12	70	theme	fungi	1919:1923	arg1	development					1904:1914	the development	1900:1914	the development of fungi	1900:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	6	71	dep	family	859:864	arg1	Ktr4					873:876	Ktr4	873:876	Ktr4	873:876	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	6	71	dep	family	859:864	arg1	Ktr7					883:886	Ktr7	883:886	Ktr7	883:886	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	6	71	dep	family	859:864	arg1	Ktr1					867:870	Ktr1	867:870	Ktr1	867:870	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	6	71	dep	family	859:864	arg1	named					842:846	named	842:846	named Kre2/Mnt1	842:856	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	7	72	theme	discovered	1018:1027	arg1	form					1029:1032	a newly discovered form	1010:1032	a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene	1010:1095	In particular, we describe a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene.
30755510	1	73	with	polysaccharides	192:206	arg1	structures					223:232	different structures	213:232	different structures in yeasts and molds	213:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	9	74	theme	marked	1372:1377	arg1	alteration					1379:1388	marked alteration	1372:1388	marked alteration of the conidial viability	1372:1414	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	13	75	theme	nutrient	1997:2004	arg1	stress					2006:2011	nutrient stress	1997:2011	nutrient stress	1997:2011	It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth.
30755510	8	76	theme	KTR4	1147:1150	arg1	gene					1152:1155	the KTR4 gene	1143:1155	the KTR4 gene	1143:1155	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	12	77	from	important	1775:1783	arg1	fumigatus					1739:1747	A. fumigatus	1736:1747	A. fumigatus	1736:1747	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	16	78	theme	essential	2350:2358	arg1	mannosyltransferases					2329:2348	two new mannosyltransferases	2321:2348	two new mannosyltransferases essential to the synthesis of the cell wall galactomannan	2321:2406	Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan.
30755510	4	79	theme	galactofuran	632:643	arg1	chains					622:627	side chains	617:627	side chains of galactofuran	617:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	9	80	theme	conidial	1397:1404	arg1	viability					1406:1414	the conidial viability	1393:1414	the conidial viability	1393:1414	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	8	81	theme	wall	1203:1206	arg1	galactomannan					1208:1220	cell wall galactomannan	1198:1220	cell wall galactomannan	1198:1220	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	5	82	theme	biological	734:743	arg1	function					745:752	biological function	734:752	biological function	734:752	Despite its use as a biomarker to diagnose invasive aspergillosis, its biosynthesis and biological function were unknown.
30755510	18	83	theme	fumigatus	2793:2801	arg1	virulence					2777:2785	the full virulence	2768:2785	the full virulence of A. fumigatus	2768:2801	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	8	84	theme	KTR7	1164:1167	arg1	gene					1169:1172	the KTR7 gene	1160:1172	the KTR7 gene	1160:1172	Biochemical analyses showed that deletion of the KTR4 gene or the KTR7 gene leads to the absence of cell wall galactomannan.
30755510	5	85	theme	invasive	689:696	arg1	aspergillosis					698:710	invasive aspergillosis	689:710	invasive aspergillosis	689:710	Despite its use as a biomarker to diagnose invasive aspergillosis, its biosynthesis and biological function were unknown.
30755510	13	86	theme	host	2017:2020	arg1	defenses					2022:2029	host defenses	2017:2029	host defenses	2017:2029	It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth.
30755510	4	87	with	mannoses	603:610	arg1	chains					622:627	side chains	617:627	side chains of galactofuran	617:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	9	88	theme	reduced	1421:1427	arg1	virulence					1429:1437	reduced virulence	1421:1437	reduced virulence in a mouse model of invasive aspergillosis	1421:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	6	89	theme	galactomannan	968:980	arg1	biosynthesis					952:963	the biosynthesis	948:963	the biosynthesis of galactomannan	948:980	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	0	90	theme	Cell	69:72	arg1	Wall					74:77	Cell Wall Mannans and Control Polarized Growth	69:114	Wall	74:77	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	1	91	theme	Fungal	142:147	arg1	polysaccharides					192:206	complex carbohydrate polysaccharides	171:206	complex carbohydrate polysaccharides with different structures in yeasts and molds	171:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	91	theme	Fungal	142:147	arg1	mannans					159:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	11	92	theme	different	1659:1667	arg1	functions					1680:1688	very different biological functions	1654:1688	very different biological functions	1654:1688	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	12	93	theme	cell	1844:1847	arg1	wall					1849:1852	The fungal cell wall	1833:1852	The fungal cell wall	1833:1852	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	93	theme	cell	1844:1847	arg1	entity					1879:1884	a complex and dynamic entity	1857:1884	a complex and dynamic entity essential for the development of fungi	1857:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	1	94	theme	wall	154:157	arg1	polysaccharides					192:206	complex carbohydrate polysaccharides	171:206	complex carbohydrate polysaccharides with different structures in yeasts and molds	171:252	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	1	94	theme	wall	154:157	arg1	mannans					159:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans	142:165	Fungal cell wall mannans are complex carbohydrate polysaccharides with different structures in yeasts and molds.
30755510	17	95	theme	parental	2581:2588	arg1	strain					2590:2595	the parental strain	2577:2595	the parental strain	2577:2595	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	4	96	theme	linear	522:527	arg1	mannan					529:534	a linear mannan	520:534	a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	520:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	11	97	theme	filamentous	1704:1714	arg1	fungi					1716:1720	filamentous fungi	1704:1720	filamentous fungi	1704:1720	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	0	98	theme	Control	91:97	arg1	Biosynthesis					53:64	the Biosynthesis	49:64	the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus	49:139	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	0	99	from	Biosynthesis	53:64	arg1	fumigatus					131:139	Aspergillus fumigatus	119:139	Aspergillus fumigatus	119:139	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	17	100	theme	conidiation	2495:2505	arg1	misregulation					2453:2465	misregulation	2453:2465	misregulation of polarization and altered conidiation	2453:2505	Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain.
30755510	9	101	theme	aspergillosis	1468:1480	arg1	model					1450:1454	a mouse model	1442:1454	a mouse model of invasive aspergillosis	1442:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	0	102	theme	KTR	4:6	arg1	Mannosyltransferases					8:27	Two KTR Mannosyltransferases	0:27	Two KTR Mannosyltransferases	0:27	Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus.
30755510	18	103	theme	full	2772:2775	arg1	virulence					2777:2785	the full virulence	2768:2785	the full virulence of A. fumigatus	2768:2801	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	18	104	from	concentration	2641:2653	arg1	wall					2667:2670	the cell wall	2658:2670	the cell wall	2658:2670	This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus.
30755510	15	105	theme	cell	2203:2206	arg1	galactomannan					2213:2225	a cell wall galactomannan	2201:2225	a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined	2201:2299	Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined.
30755510	3	106	theme	major	386:390	arg1	structure					399:407	the major mannan structure	382:407	the major mannan structure	382:407	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	3	106	theme	major	386:390	arg1	galactomannan					414:426	a galactomannan	412:426	a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core	412:489	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	6	107	from	function	799:806	arg1	fumigatus					895:903	A. fumigatus	892:903	A. fumigatus	892:903	Here, we have investigated the function of three members of the Ktr (also named Kre2/Mnt1) family (Ktr1, Ktr4, and Ktr7) in A. fumigatus and show that two of them are required for the biosynthesis of galactomannan.
30755510	11	108	theme	orthologous	1604:1614	arg1	genes					1616:1620	orthologous genes	1604:1620	orthologous genes	1604:1620	This study provided another confirmation that orthologous genes can code for proteins that have very different biological functions in yeasts and filamentous fungi.
30755510	16	109	theme	new	2325:2327	arg1	mannosyltransferases					2329:2348	two new mannosyltransferases	2321:2348	two new mannosyltransferases essential to the synthesis of the cell wall galactomannan	2321:2406	Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan.
30755510	3	110	theme	cell	476:479	arg1	core					486:489	the β-1,3-glucan-chitin cell wall core	452:489	the β-1,3-glucan-chitin cell wall core	452:489	In Aspergillus fumigatus, the major mannan structure is a galactomannan that is cross-linked to the β-1,3-glucan-chitin cell wall core.
30755510	7	111	theme	KTR4	1087:1090	arg1	gene					1092:1095	the KTR4 gene	1083:1095	the KTR4 gene	1083:1095	In particular, we describe a newly discovered form of α-1,2-mannosyltransferase activity encoded by the KTR4 gene.
30755510	15	112	theme	Aspergillus	2170:2180	arg1	fumigatus					2182:2190	Aspergillus fumigatus	2170:2190	Aspergillus fumigatus	2170:2190	Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined.
30755510	9	113	from	defects	1324:1330	arg1	growth					1345:1350	polarized growth	1335:1350	polarized growth	1335:1350	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	113	from	defects	1324:1330	arg1	conidiation					1359:1369	conidiation	1359:1369	conidiation	1359:1369	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	113	from	defects	1324:1330	arg1	virulence					1429:1437	reduced virulence	1421:1437	reduced virulence in a mouse model of invasive aspergillosis	1421:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	9	113	from	defects	1324:1330	arg1	alteration					1379:1388	marked alteration	1372:1388	marked alteration of the conidial viability	1372:1414	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	4	114	theme	repeating	543:551	arg1	unit					553:556	a repeating unit	541:556	a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	541:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	13	115	theme	fungal	1936:1941	arg1	pathogens					1943:1951	fungal pathogens	1936:1951	fungal pathogens	1936:1951	It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth.
30755510	9	116	theme	growth	1302:1307	arg1	phenotype					1309:1317	a severe growth phenotype	1293:1317	a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis	1293:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	4	117	link	α1,2-linked	591:601	arg1	mannoses					603:610	four α1,6-linked and α1,2-linked mannoses	570:610	four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	570:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	4	118	theme	side	617:620	arg1	chains					622:627	side chains	617:627	side chains of galactofuran	617:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	4	119	link	α1,6-linked	575:585	arg1	mannoses					603:610	four α1,6-linked and α1,2-linked mannoses	570:610	four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran	570:643	This polymer is composed of a linear mannan with a repeating unit composed of four α1,6-linked and α1,2-linked mannoses with side chains of galactofuran.
30755510	9	120	from	alteration	1379:1388	arg1	model					1450:1454	a mouse model	1442:1454	a mouse model of invasive aspergillosis	1442:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
30755510	12	121	theme	dynamic	1871:1877	arg1	wall					1849:1852	The fungal cell wall	1833:1852	The fungal cell wall	1833:1852	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	12	121	theme	dynamic	1871:1877	arg1	entity					1879:1884	a complex and dynamic entity	1857:1884	a complex and dynamic entity essential for the development of fungi	1857:1923	Moreover, in A. fumigatus, cell wall mannans are as important structurally as β-glucans and chitin.IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi.
30755510	9	122	with	phenotype	1309:1317	arg1	defects					1324:1330	defects	1324:1330	defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis	1324:1480	In comparison to parental strains, the Δktr4 and Δktr7 mutants showed a severe growth phenotype with defects in polarized growth and in conidiation, marked alteration of the conidial viability, and reduced virulence in a mouse model of invasive aspergillosis.
31826395	8	0	theme	licorice	1187:1194	arg1	residues					1196:1203	licorice residues	1187:1203	licorice residues	1187:1203	This study would expend licorice residues as potential materials for CNF and its applications.
31826395	8	0	theme	licorice	1187:1194	arg1	materials					1218:1226	potential materials	1208:1226	potential materials for CNF and its applications	1208:1255	This study would expend licorice residues as potential materials for CNF and its applications.
31826395	7	1	theme	ETCNF	955:959	arg1	film					971:974	ETCNF@LNPs-5.0% film	955:974	ETCNF@LNPs-5.0% film	955:974	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	2	theme	colloidal	693:701	arg1	stability					703:711	a highly relative colloidal stability	675:711	a highly relative colloidal stability	675:711	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	7	3	theme	ETCNF	943:947	arg1	film					949:952	ETCNF film	943:952	ETCNF film	943:952	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	1	4	theme	industrial	169:178	arg1	residues					188:195	industrial biomass residues	169:195	industrial biomass residues	169:195	It is important to make full utilization of industrial biomass residues.
31826395	6	5	theme	nanofibrillar	647:659	arg1	structure					661:669	clear nanofibrillar structure	641:669	clear nanofibrillar structure	641:669	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	3	6	theme	pulp	404:407	arg1	homogenization					382:395	homogenization	382:395	homogenization of the pulp (ETCNF)	382:415	Cellulose nanofibril was obtained by enzymatic pretreatment followed by homogenization of the pulp (ETCNF).
31826395	7	7	theme	light	1142:1146	arg1	transmittance					1148:1160	similar light transmittance	1134:1160	similar light transmittance	1134:1160	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	7	8	theme	higher	983:988	arg1	ability					1002:1008	higher UV-blocking ability	983:1008	higher UV-blocking ability	983:1008	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	9	theme	clear	641:645	arg1	structure					661:669	clear nanofibrillar structure	641:669	clear nanofibrillar structure	641:669	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	0	10	theme	natural	102:108	arg1	nanoparticles					110:122	natural nanoparticles	102:122	natural nanoparticles	102:122	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	6	11	theme	oxidized	802:809	arg1	one					811:813	TEMPO-medicated oxidized one	786:813	TEMPO-medicated oxidized one	786:813	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	4	12	theme	pretreatment	443:454	arg1	effects					422:428	The effects	418:428	The effects of enzymatic pretreatment on ETCNF	418:463	The effects of enzymatic pretreatment on ETCNF were investigated.
31826395	7	13	theme	@	960:960	arg1	film					971:974	ETCNF@LNPs-5.0% film	955:974	ETCNF@LNPs-5.0% film	955:974	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	4	14	theme	enzymatic	433:441	arg1	pretreatment					443:454	enzymatic pretreatment	433:454	enzymatic pretreatment	433:454	The effects of enzymatic pretreatment on ETCNF were investigated.
31826395	6	15	theme	crystallinity	732:744	arg1	index					746:750	a much higher crystallinity index	718:750	a much higher crystallinity index	718:750	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	1	16	theme	biomass	180:186	arg1	residues					188:195	industrial biomass residues	169:195	industrial biomass residues	169:195	It is important to make full utilization of industrial biomass residues.
31826395	8	17	theme	potential	1208:1216	arg1	residues					1196:1203	licorice residues	1187:1203	licorice residues	1187:1203	This study would expend licorice residues as potential materials for CNF and its applications.
31826395	8	17	theme	potential	1208:1216	arg1	materials					1218:1226	potential materials	1208:1226	potential materials for CNF and its applications	1208:1255	This study would expend licorice residues as potential materials for CNF and its applications.
31826395	0	18	from	residues	47:54	arg1	nanofibril					22:31	Fabricating cellulose nanofibril	0:31	Fabricating cellulose nanofibril from licorice residues	0:54	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	0	18	from	residues	47:54	arg1	composite					74:82	its cellulose composite	60:82	its cellulose composite	60:82	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	7	19	theme	similar	1134:1140	arg1	transmittance					1148:1160	similar light transmittance	1134:1160	similar light transmittance	1134:1160	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	20	theme	composite	830:838	arg1	films					840:844	the cellulose composite films	816:844	the cellulose composite films incorporated with CHN or LNPs	816:874	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	4	21	from	effects	422:428	arg1	ETCNF					459:463	ETCNF	459:463	ETCNF	459:463	The effects of enzymatic pretreatment on ETCNF were investigated.
31826395	6	22	theme	higher	725:730	arg1	index					746:750	a much higher crystallinity index	718:750	a much higher crystallinity index	718:750	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	1	23	theme	residues	188:195	arg1	utilization					154:164	full utilization	149:164	full utilization of industrial biomass residues	149:195	It is important to make full utilization of industrial biomass residues.
31826395	0	24	theme	cellulose	12:20	arg1	nanofibril					22:31	Fabricating cellulose nanofibril	0:31	Fabricating cellulose nanofibril from licorice residues	0:54	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	7	25	theme	mechanical	1108:1117	arg1	properties					1119:1128	improved mechanical properties	1099:1128	improved mechanical properties	1099:1128	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	26	theme	cellulose	820:828	arg1	films					840:844	the cellulose composite films	816:844	the cellulose composite films incorporated with CHN or LNPs	816:874	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	7	27	theme	CHN-5.0	1078:1084	arg1	%					1085:1085	%	1085:1085	%	1085:1085	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	0	28	theme	Fabricating	0:10	arg1	nanofibril					22:31	Fabricating cellulose nanofibril	0:31	Fabricating cellulose nanofibril from licorice residues	0:54	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	7	29	theme	reduced	1037:1043	arg1	transmittance					1051:1063	reduced light transmittance	1037:1063	reduced light transmittance	1037:1063	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	7	30	theme	LNPs-5.0	961:968	arg1	%					969:969	%	969:969	%	969:969	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	31	theme	good	886:889	arg1	stability					899:907	good thermal stability	886:907	good thermal stability	886:907	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	3	32	theme	Cellulose	310:318	arg1	nanofibril					320:329	Cellulose nanofibril	310:329	Cellulose nanofibril	310:329	Cellulose nanofibril was obtained by enzymatic pretreatment followed by homogenization of the pulp (ETCNF).
31826395	7	33	theme	UV-blocking	990:1000	arg1	ability					1002:1008	higher UV-blocking ability	983:1008	higher UV-blocking ability	983:1008	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	7	34	theme	improved	1099:1106	arg1	properties					1119:1128	improved mechanical properties	1099:1128	improved mechanical properties	1099:1128	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	7	35	theme	%	1085:1085	arg1	film					1087:1090	ETCNF@CHN-5.0% film	1072:1090	ETCNF@CHN-5.0% film	1072:1090	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	5	36	theme	lignin	514:519	arg1	LNPs					536:539	LNPs	536:539	LNPs	536:539	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	5	36	theme	lignin	514:519	arg1	nanoparticles					521:533	Chitosan nanofibril (CHN) and lignin nanoparticles	484:533	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs)	484:540	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	0	37	theme	licorice	38:45	arg1	residues					47:54	licorice residues	38:54	licorice residues	38:54	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	2	38	theme	soda-anthraquinone	242:259	arg1	pulping					261:267	soda-anthraquinone pulping	242:267	soda-anthraquinone pulping followed by peroxyacetic acid bleaching	242:307	Pulp was prepared from licorice residues by soda-anthraquinone pulping followed by peroxyacetic acid bleaching.
31826395	7	39	theme	@	1077:1077	arg1	film					1087:1090	ETCNF@CHN-5.0% film	1072:1090	ETCNF@CHN-5.0% film	1072:1090	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	6	40	theme	thermal	891:897	arg1	stability					899:907	good thermal stability	886:907	good thermal stability	886:907	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	5	41	theme	ETCNF	569:573	arg1	composites					575:584	ETCNF composites	569:584	ETCNF composites	569:584	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	3	42	theme	enzymatic	347:355	arg1	pretreatment					357:368	enzymatic pretreatment	347:368	enzymatic pretreatment followed by homogenization of the pulp (ETCNF)	347:415	Cellulose nanofibril was obtained by enzymatic pretreatment followed by homogenization of the pulp (ETCNF).
31826395	2	43	theme	licorice	221:228	arg1	residues					230:237	licorice residues	221:237	licorice residues	221:237	Pulp was prepared from licorice residues by soda-anthraquinone pulping followed by peroxyacetic acid bleaching.
31826395	6	44	theme	TEMPO-medicated	786:800	arg1	one					811:813	TEMPO-medicated oxidized one	786:813	TEMPO-medicated oxidized one	786:813	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	5	45	used	used	560:563	arg2	LNPs					536:539	LNPs	536:539	LNPs	536:539	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	5	45	used	used	560:563	arg2	nanoparticles					521:533	Chitosan nanofibril (CHN) and lignin nanoparticles	484:533	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs)	484:540	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	7	46	theme	%	969:969	arg1	film					971:974	ETCNF@LNPs-5.0% film	955:974	ETCNF@LNPs-5.0% film	955:974	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	2	47	theme	acid	294:297	arg1	bleaching					299:307	peroxyacetic acid bleaching	281:307	peroxyacetic acid bleaching	281:307	Pulp was prepared from licorice residues by soda-anthraquinone pulping followed by peroxyacetic acid bleaching.
31826395	7	48	theme	light	1045:1049	arg1	transmittance					1051:1063	reduced light transmittance	1037:1063	reduced light transmittance	1037:1063	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	5	49	theme	Chitosan	484:491	arg1	CHN					505:507	CHN	505:507	CHN	505:507	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	5	49	theme	Chitosan	484:491	arg1	nanofibril					493:502	Chitosan nanofibril	484:502	Chitosan nanofibril (CHN)	484:508	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	7	50	theme	ETCNF	1072:1076	arg1	film					1087:1090	ETCNF@CHN-5.0% film	1072:1090	ETCNF@CHN-5.0% film	1072:1090	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	2	51	theme	peroxyacetic	281:292	arg1	bleaching					299:307	peroxyacetic acid bleaching	281:307	peroxyacetic acid bleaching	281:307	Pulp was prepared from licorice residues by soda-anthraquinone pulping followed by peroxyacetic acid bleaching.
31826395	5	52	theme	nanofibril	493:502	arg1	LNPs					536:539	LNPs	536:539	LNPs	536:539	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	5	52	theme	nanofibril	493:502	arg1	nanoparticles					521:533	Chitosan nanofibril (CHN) and lignin nanoparticles	484:533	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs)	484:540	Chitosan nanofibril (CHN) and lignin nanoparticles (LNPs) were prepared and used for ETCNF composites, respectively.
31826395	7	53	theme	thermal	1014:1020	arg1	stability					1022:1030	thermal stability	1014:1030	thermal stability	1014:1030	Compared with ETCNF film, ETCNF@LNPs-5.0% film showed higher UV-blocking ability and thermal stability, but reduced light transmittance, while ETCNF@CHN-5.0% film showed improved mechanical properties and similar light transmittance.
31826395	1	54	theme	full	149:152	arg1	utilization					154:164	full utilization	149:164	full utilization of industrial biomass residues	149:195	It is important to make full utilization of industrial biomass residues.
31826395	6	55	theme	relative	684:691	arg1	stability					703:711	a highly relative colloidal stability	675:711	a highly relative colloidal stability	675:711	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
31826395	0	56	theme	cellulose	64:72	arg1	composite					74:82	its cellulose composite	60:82	its cellulose composite	60:82	Fabricating cellulose nanofibril from licorice residues and its cellulose composite incorporated with natural nanoparticles.
31826395	6	57	theme	thermal	756:762	arg1	stability					764:772	thermal stability	756:772	thermal stability	756:772	The results showed that ETCNF exhibited clear nanofibrillar structure and a highly relative colloidal stability, and a much higher crystallinity index and thermal stability compared to TEMPO-medicated oxidized one; the cellulose composite films incorporated with CHN or LNPs exhibited good thermal stability and hydrophobicity.
29438750	10	0	theme	liquid	955:960	arg1	behavior					975:982	a liquid viscoelastic behavior	953:982	a liquid viscoelastic behavior in 1% and 2% concentrations	953:1010	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	4	1	theme	rhamnose	415:422	arg1	glucose					428:434	glucose	428:434	glucose	428:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	arabinose					362:370	arabinose	362:370	arabinose	362:370	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	amount					396:401	a small amount	388:401	a small amount of mannose, rhamnose and glucose	388:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	galactose					373:381	galactose	373:381	galactose	373:381	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	mannose					406:412	mannose	406:412	mannose	406:412	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	composition					339:349	The monosaccharide composition	320:349	The monosaccharide composition	320:349	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	1	theme	rhamnose	415:422	arg1	rhamnose					415:422	rhamnose	415:422	rhamnose	415:422	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	10	2	theme	NaCl	1089:1092	arg1	addition					1077:1084	the addition	1073:1084	3% concentration as well as the addition of NaCl and CaCl2	1045:1102	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	10	2	theme	NaCl	1089:1092	arg1	concentration					1048:1060	concentration	1048:1060	3% concentration as well as the addition of NaCl and CaCl2	1045:1102	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	10	3	theme	CaCl2	1098:1102	arg1	addition					1077:1084	the addition	1073:1084	3% concentration as well as the addition of NaCl and CaCl2	1045:1102	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	10	3	theme	CaCl2	1098:1102	arg1	concentration					1048:1060	concentration	1048:1060	3% concentration as well as the addition of NaCl and CaCl2	1045:1102	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	7	4	theme	tested	642:647	arg1	range					660:664	the tested shear rate range	638:664	the tested shear rate range	638:664	LP showed shear-thinning behavior during the tested shear rate range.
29438750	8	5	used	used	691:694	arg2	model					681:685	The power-law model	667:685	The power-law model	667:685	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	4	6	theme	monosaccharide	324:337	arg1	composition					339:349	The monosaccharide composition	320:349	The monosaccharide composition	320:349	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	6	theme	monosaccharide	324:337	arg1	arabinose					362:370	arabinose	362:370	arabinose	362:370	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	8	7	theme	behavior	747:754	arg1	index					756:760	its flow behavior index	738:760	its flow behavior index	738:760	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	7	8	theme	shear	649:653	arg1	range					660:664	the tested shear rate range	638:664	the tested shear rate range	638:664	LP showed shear-thinning behavior during the tested shear rate range.
29438750	9	9	theme	LP	840:841	arg1	viscosity					827:835	The viscosity	823:835	The viscosity of LP	823:841	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	9	9	theme	LP	840:841	arg1	stable					894:899	stable	894:899	stable	894:899	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	4	10	theme	glucose	428:434	arg1	glucose					428:434	glucose	428:434	glucose	428:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	arabinose					362:370	arabinose	362:370	arabinose	362:370	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	amount					396:401	a small amount	388:401	a small amount of mannose, rhamnose and glucose	388:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	galactose					373:381	galactose	373:381	galactose	373:381	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	mannose					406:412	mannose	406:412	mannose	406:412	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	composition					339:349	The monosaccharide composition	320:349	The monosaccharide composition	320:349	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	10	theme	glucose	428:434	arg1	rhamnose					415:422	rhamnose	415:422	rhamnose	415:422	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	8	11	theme	flow	742:745	arg1	index					756:760	its flow behavior index	738:760	its flow behavior index	738:760	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	8	12	theme	flow	712:715	arg1	behavior					717:724	the flow behavior	708:724	the flow behavior of LP	708:730	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	10	13	theme	%	988:988	arg1	concentrations					997:1010	1% and 2% concentrations	987:1010	1% and 2% concentrations	987:1010	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	9	14	with	stable	894:899	arg1	treatment					911:919	heat treatment	906:919	heat treatment	906:919	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	5	15	dep	showed	472:477	arg1	structure					501:509	porous network structure	486:509	porous network structure	486:509	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	10	16	theme	%	1046:1046	arg1	concentration					1048:1060	concentration	1048:1060	3% concentration as well as the addition of NaCl and CaCl2	1045:1102	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	2	17	theme	chemical	136:143	arg1	composition					145:155	Its chemical composition	132:155	Its chemical composition	132:155	Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated.
29438750	1	18	theme	Litchi	73:78	arg1	LP					96:97	LP	96:97	LP	96:97	Litchi polysaccharide (LP) was extracted from litchi pulp.
29438750	1	18	theme	Litchi	73:78	arg1	polysaccharide					80:93	Litchi polysaccharide	73:93	Litchi polysaccharide (LP)	73:98	Litchi polysaccharide (LP) was extracted from litchi pulp.
29438750	9	19	theme	acidic	859:864	arg1	pH2-4					878:882	pH2-4	878:882	pH2-4	878:882	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	9	19	theme	acidic	859:864	arg1	conditions					866:875	acidic conditions	859:875	acidic conditions (pH2-4)	859:883	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	3	20	theme	uronic	253:258	arg1	acid					260:263	uronic acid	253:263	uronic acid (41.18%)	253:272	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	20	theme	uronic	253:258	arg1	%					271:271	41.18%	266:271	41.18%	266:271	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	4	21	theme	mannose	406:412	arg1	glucose					428:434	glucose	428:434	glucose	428:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	arabinose					362:370	arabinose	362:370	arabinose	362:370	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	amount					396:401	a small amount	388:401	a small amount of mannose, rhamnose and glucose	388:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	galactose					373:381	galactose	373:381	galactose	373:381	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	mannose					406:412	mannose	406:412	mannose	406:412	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	composition					339:349	The monosaccharide composition	320:349	The monosaccharide composition	320:349	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	21	theme	mannose	406:412	arg1	rhamnose					415:422	rhamnose	415:422	rhamnose	415:422	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	5	22	theme	porous	486:491	arg1	structure					501:509	porous network structure	486:509	porous network structure	486:509	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	8	23	theme	consistency	766:776	arg1	index					778:782	consistency index	766:782	consistency index	766:782	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	0	24	theme	Chemical	0:7	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of polysaccharides from litchi pulp	0:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	5	25	theme	network	493:499	arg1	structure					501:509	porous network structure	486:509	porous network structure	486:509	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	6	26	theme	LP	512:513	arg1	concentration					515:527	LP concentration	512:527	LP concentration	512:527	LP concentration had no effect on its zeta potential value while salts reduced them.
29438750	10	27	theme	%	995:995	arg1	concentrations					997:1010	1% and 2% concentrations	987:1010	1% and 2% concentrations	987:1010	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	0	28	theme	rheological	13:23	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of polysaccharides from litchi pulp	0:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	2	29	theme	zeta	174:177	arg1	potential					179:187	zeta potential	174:187	zeta potential	174:187	Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated.
29438750	8	30	theme	different	797:805	arg1	concentrations					807:820	different concentrations	797:820	different concentrations	797:820	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	0	31	theme	polysaccharides	39:53	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of polysaccharides from litchi pulp	0:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	6	32	theme	zeta	550:553	arg1	value					565:569	its zeta potential value	546:569	its zeta potential value	546:569	LP concentration had no effect on its zeta potential value while salts reduced them.
29438750	10	33	from	behavior	975:982	arg1	concentrations					997:1010	1% and 2% concentrations	987:1010	1% and 2% concentrations	987:1010	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	4	34	theme	small	390:394	arg1	mannose					406:412	mannose	406:412	mannose	406:412	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	34	theme	small	390:394	arg1	amount					396:401	a small amount	388:401	a small amount of mannose, rhamnose and glucose	388:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	34	theme	small	390:394	arg1	glucose					428:434	glucose	428:434	glucose	428:434	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	4	34	theme	small	390:394	arg1	rhamnose					415:422	rhamnose	415:422	rhamnose	415:422	The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose.
29438750	0	35	theme	litchi	60:65	arg1	pulp					67:70	litchi pulp	60:70	litchi pulp	60:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	3	36	theme	neutral	275:281	arg1	%					295:295	42.23%	290:295	42.23%	290:295	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	36	theme	neutral	275:281	arg1	sugar					283:287	neutral sugar	275:287	neutral sugar (42.23%)	275:296	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	0	37	from	pulp	67:70	arg1	polysaccharides					39:53	polysaccharides	39:53	polysaccharides from litchi pulp	39:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	0	37	from	pulp	67:70	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of polysaccharides from litchi pulp	0:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	10	38	theme	viscoelastic	962:973	arg1	behavior					975:982	a liquid viscoelastic behavior	953:982	a liquid viscoelastic behavior in 1% and 2% concentrations	953:1010	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	2	39	theme	viscoelastic	199:210	arg1	behavior					212:219	viscoelastic behavior	199:219	viscoelastic behavior	199:219	Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated.
29438750	7	40	theme	shear-thinning	607:620	arg1	behavior					622:629	shear-thinning behavior	607:629	shear-thinning behavior	607:629	LP showed shear-thinning behavior during the tested shear rate range.
29438750	6	41	contain	had	529:531	arg1	concentration					515:527	LP concentration	512:527	LP concentration	512:527	LP concentration had no effect on its zeta potential value while salts reduced them.
29438750	6	41	contain	had	529:531	arg2	effect					536:541	no effect	533:541	no effect	533:541	LP concentration had no effect on its zeta potential value while salts reduced them.
29438750	1	42	theme	litchi	119:124	arg1	pulp					126:129	litchi pulp	119:129	litchi pulp	119:129	Litchi polysaccharide (LP) was extracted from litchi pulp.
29438750	9	43	theme	heat	906:909	arg1	treatment					911:919	heat treatment	906:919	heat treatment	906:919	The viscosity of LP increased under acidic conditions (pH2-4), but was stable with heat treatment.
29438750	10	44	theme	3	1045:1045	arg1	%					1046:1046	%	1046:1046	%	1046:1046	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	10	45	theme	LP	926:927	arg1	dispersion					929:938	The LP dispersion	922:938	The LP dispersion	922:938	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
29438750	5	46	theme	Scanning	437:444	arg1	microscopy					455:464	Scanning electron microscopy	437:464	Scanning electron microscopy (SEM)	437:470	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	5	46	theme	Scanning	437:444	arg1	SEM					467:469	SEM	467:469	SEM	467:469	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	6	47	theme	potential	555:563	arg1	value					565:569	its zeta potential value	546:569	its zeta potential value	546:569	LP concentration had no effect on its zeta potential value while salts reduced them.
29438750	8	48	theme	power-law	671:679	arg1	model					681:685	The power-law model	667:685	The power-law model	667:685	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	8	49	theme	LP	729:730	arg1	behavior					717:724	the flow behavior	708:724	the flow behavior of LP	708:730	The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations.
29438750	5	50	theme	electron	446:453	arg1	microscopy					455:464	Scanning electron microscopy	437:464	Scanning electron microscopy (SEM)	437:470	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	5	50	theme	electron	446:453	arg1	SEM					467:469	SEM	467:469	SEM	467:469	Scanning electron microscopy (SEM) showed LP was porous network structure.
29438750	3	51	contain	contained	243:251	arg2	acid					260:263	uronic acid	253:263	uronic acid (41.18%)	253:272	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg2	%					271:271	41.18%	266:271	41.18%	266:271	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg2	%					316:316	2.72%	312:316	2.72%	312:316	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg2	%					295:295	42.23%	290:295	42.23%	290:295	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg2	sugar					283:287	neutral sugar	275:287	neutral sugar (42.23%)	275:296	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg1	LP					240:241	LP	240:241	LP	240:241	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	3	51	contain	contained	243:251	arg2	protein					303:309	protein	303:309	protein (2.72%)	303:317	LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%).
29438750	7	52	theme	rate	655:658	arg1	range					660:664	the tested shear rate range	638:664	the tested shear rate range	638:664	LP showed shear-thinning behavior during the tested shear rate range.
29438750	0	53	from	properties	25:34	arg1	pulp					67:70	litchi pulp	60:70	litchi pulp	60:70	Chemical and rheological properties of polysaccharides from litchi pulp.
29438750	10	54	theme	elastic	1030:1036	arg1	gel					1038:1040	an elastic gel	1027:1040	an elastic gel	1027:1040	The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2.
30238739	17	0	theme	protein-100	2127:2137	arg1	staining					2158:2165	soluble protein-100 immunofluorescence staining	2119:2165	soluble protein-100 immunofluorescence staining	2119:2165	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	9	1	dep	Results	1003:1009	arg1	showed					1029:1034	showed	1029:1034	showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1	1029:1152	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	20	2	theme	group	2692:2696	arg1	B					2698:2698	group B	2692:2698	group B	2692:2698	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	23	3	theme	composite	2989:2997	arg1	membranes					2999:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	21	4	theme	membranes	2776:2784	arg1	performance					2757:2767	the degradation performance	2741:2767	the degradation performance of the membranes modified by acetylation	2741:2808	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	21	4	theme	membranes	2776:2784	arg1	better					2814:2819	better	2814:2819	better	2814:2819	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	20	5	theme	degradation	2603:2613	arg1	higher					2671:2676	higher	2671:2676	higher	2671:2676	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	5	theme	degradation	2603:2613	arg1	rates					2615:2619	the degradation rates	2599:2619	the degradation rates of membranes in groups A and C	2599:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	21	6	theme	same	2863:2866	arg1	condition					2868:2876	the same condition	2859:2876	the same condition	2859:2876	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	4	7	dep	membranes	450:458	arg1	The					437:439	The	437:439	The	437:439	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	18	8	theme	cells	2262:2266	arg1	proliferation					2237:2249	the proliferation	2233:2249	the proliferation of Schwann cells after 2 days and 4 days of group A	2233:2301	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	18	8	theme	cells	2262:2266	arg1	more					2307:2310	more	2307:2310	more	2307:2310	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	23	9	theme	Nano	2973:2976	arg1	membranes					2999:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	20	10	theme	membranes	2568:2576	arg1	degradation					2553:2563	The degradation	2549:2563	The degradation of membranes in vitro	2549:2585	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	5	11	theme	group	646:650	arg1	PPy					616:618	the nano PPy	607:618	the nano PPy	607:618	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	5	11	theme	group	646:650	arg1	membranes					628:636	the membranes	624:636	the membranes of each group	624:650	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	15	12	from	surface	1785:1791	arg1	A					1815:1815	group A	1809:1815	group A	1809:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	21	13	theme	chitosan	2834:2841	arg1	membranes					2843:2851	chitosan membranes	2834:2851	chitosan membranes	2834:2851	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	12	14	theme	turn	1325:1328	arg1	chitosan					1330:1337	turn chitosan	1325:1337	turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C	1325:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	9	15	from	PPy	1106:1108	arg1	peaks					1083:1087	the characteristic vibrational absorption peaks	1041:1087	the characteristic vibrational absorption peaks of C=C from nano PPy	1041:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	11	16	theme	nano	1267:1270	arg1	particles					1276:1284	The nano PPy particles	1263:1284	The nano PPy particles	1263:1284	The nano PPy particles were synthesized.
30238739	15	17	from	membranes	1796:1804	arg1	A					1815:1815	group A	1809:1815	group A	1809:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	3	18	mod	modified	331:338	arg1	membranes					311:319	The membranes	307:319	The membranes	307:319	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	18	mod	modified	331:338	arg3	acetylation					343:353	acetylation	343:353	acetylation	343:353	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	4	19	theme	group	461:465	arg1	membranes					450:458	chitosan membranes	441:458	chitosan membranes (group B)	441:468	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	4	19	theme	group	461:465	arg1	B					467:467	group B	461:467	group B	461:467	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	18	20	theme	cell	2207:2210	arg1	counting					2212:2219	The cell counting	2203:2219	The cell counting	2203:2219	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	20	21	from	rates	2615:2619	arg1	groups					2637:2642	groups A and C	2637:2650	groups A and C	2637:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	21	from	rates	2615:2619	arg1	C					2650:2650	C	2650:2650	C	2650:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	21	from	rates	2615:2619	arg1	A					2644:2644	A	2644:2644	A	2644:2644	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	15	22	theme	nano	1737:1740	arg1	particles					1746:1754	The nano PPy particles	1733:1754	The nano PPy particles uniformly distributed on the surface of membranes in group A	1733:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	14	23	from	conductivity	1580:1591	arg1	A					1615:1615	group A	1609:1615	group A	1609:1615	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	13	24	theme	Ⅱ	1480:1480	arg1	band					1482:1485	the amide Ⅱ band	1470:1485	the amide Ⅱ band	1470:1485	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after acetylated modification.
30238739	19	25	contain	had	2455:2457	arg1	membranes					2445:2453	the nano PPy/chitin composite membranes	2415:2453	the nano PPy/chitin composite membranes	2415:2453	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	19	25	contain	had	2455:2457	arg2	ability					2466:2472	better ability	2459:2472	better ability of adhesion and proliferation	2459:2502	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	7	26	theme	immunofluorescence	888:905	arg1	staining					907:914	immunofluorescence staining	888:914	immunofluorescence staining	888:914	Schwann cells were co-cultured in vitro with each group membranes to observe the biocompatibility by inverted microscope observing, living cell staining, cell counting, and immunofluorescence staining.
30238739	17	27	theme	microscope	2181:2190	arg1	observing					2192:2200	inverted microscope observing	2172:2200	inverted microscope observing	2172:2200	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	20	28	theme	time	2707:2710	arg1	points					2712:2717	all time points	2703:2717	all time points	2703:2717	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	1	29	theme	polypyrrole	108:118	arg1	membrane					143:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	Objective To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.
30238739	1	30	theme	composite	133:141	arg1	membrane					143:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	Objective To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.
30238739	20	31	dep	groups	2637:2642	arg1	groups					2637:2642	groups A and C	2637:2650	groups A and C	2637:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	31	dep	groups	2637:2642	arg1	C					2650:2650	C	2650:2650	C	2650:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	31	dep	groups	2637:2642	arg1	A					2644:2644	A	2644:2644	A	2644:2644	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	10	32	theme	electron	1164:1171	arg1	observation					1184:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	19	33	theme	nano	2419:2422	arg1	membranes					2445:2453	the nano PPy/chitin composite membranes	2415:2453	the nano PPy/chitin composite membranes	2415:2453	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	17	34	theme	diacetate	2089:2097	arg1	staining					2109:2116	fluorescein diacetate live cell staining	2077:2116	fluorescein diacetate live cell staining	2077:2116	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	23	35	theme	good	3057:3060	arg1	biocompatibility					3062:3077	good biocompatibility	3057:3077	good biocompatibility	3057:3077	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	9	36	theme	FT-IR	1015:1019	arg1	spectra					1021:1027	The FT-IR spectra	1011:1027	The FT-IR spectra	1011:1027	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	22	37	dep	Conclusion	2879:2888	arg1	modified					2935:2942	modified	2935:2942	modified by acetylation successfully	2935:2970	Conclusion The nano PPy and chitosan can be blended and modified by acetylation successfully.
30238739	22	37	dep	Conclusion	2879:2888	arg1	blended					2923:2929	blended	2923:2929	blended	2923:2929	Conclusion The nano PPy and chitosan can be blended and modified by acetylation successfully.
30238739	3	38	theme	experimental	366:377	arg1	membranes					379:387	the experimental membranes	362:387	the experimental membranes (nano PPy/chitin composite membranes, group A)	362:434	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	17	39	theme	cell	2104:2107	arg1	staining					2109:2116	fluorescein diacetate live cell staining	2077:2116	fluorescein diacetate live cell staining	2077:2116	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	15	40	theme	group	1809:1813	arg1	A					1815:1815	group A	1809:1815	group A	1809:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	15	41	from	membranes	1868:1876	arg1	groups					1889:1894	control groups	1881:1894	control groups	1881:1894	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	14	42	theme	Conductivity	1546:1557	arg1	test					1559:1562	Conductivity test	1546:1562	Conductivity test	1546:1562	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	15	43	theme	membranes	1796:1804	arg1	surface					1785:1791	the surface	1781:1791	the surface of membranes in group A	1781:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	10	44	theme	particles	1231:1239	arg1	nm					1259:1260	about 100-200 nm	1245:1260	about 100-200 nm	1245:1260	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	10	44	theme	particles	1231:1239	arg1	size					1214:1217	the size	1210:1217	the size of nano PPy particles	1210:1239	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	19	45	theme	composite	2435:2443	arg1	membranes					2445:2453	the nano PPy/chitin composite membranes	2415:2453	the nano PPy/chitin composite membranes	2415:2453	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	5	46	theme	electron	547:554	arg1	microscopy					556:565	Scanning electron microscopy	538:565	Scanning electron microscopy	538:565	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	3	47	theme	PPy/chitin	395:404	arg1	membranes					416:424	nano PPy/chitin composite membranes	390:424	nano PPy/chitin composite membranes	390:424	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	47	theme	PPy/chitin	395:404	arg1	A					433:433	group A	427:433	group A	427:433	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	4	48	theme	chitin	474:479	arg1	ones					481:484	chitin ones	474:484	chitin ones (group C)	474:494	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	4	48	theme	chitin	474:479	arg1	C					493:493	group C	487:493	group C	487:493	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	16	49	theme	electrical	1967:1976	arg1	conductivity					1978:1989	electrical conductivity	1967:1989	electrical conductivity	1967:1989	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	9	50	theme	vibrational	1060:1070	arg1	peaks					1083:1087	the characteristic vibrational absorption peaks	1041:1087	the characteristic vibrational absorption peaks of C=C from nano PPy	1041:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	1	51	theme	nano	103:106	arg1	membrane					143:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	Objective To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.
30238739	16	52	with	membranes	1952:1960	arg1	conductivity					1978:1989	electrical conductivity	1967:1989	electrical conductivity	1967:1989	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	3	53	theme	group	427:431	arg1	membranes					416:424	nano PPy/chitin composite membranes	390:424	nano PPy/chitin composite membranes	390:424	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	53	theme	group	427:431	arg1	A					433:433	group A	427:433	group A	427:433	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	18	54	theme	group	2295:2299	arg1	A					2301:2301	group A	2295:2301	group A	2295:2301	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	9	55	theme	C=C	1092:1094	arg1	peaks					1083:1087	the characteristic vibrational absorption peaks	1041:1087	the characteristic vibrational absorption peaks of C=C from nano PPy	1041:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	0	56	theme	nano	37:40	arg1	membrane					71:78	nano polypyrrole/chitin composite membrane	37:78	nano polypyrrole/chitin composite membrane	37:78	[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].
30238739	13	57	theme	acetylated	1521:1530	arg1	modification					1532:1543	acetylated modification	1521:1543	acetylated modification	1521:1543	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after acetylated modification.
30238739	9	58	theme	nano	1101:1104	arg1	PPy					1106:1108	nano PPy	1101:1108	nano PPy	1101:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	0	59	theme	composite	61:69	arg1	membrane					71:78	nano polypyrrole/chitin composite membrane	37:78	nano polypyrrole/chitin composite membrane	37:78	[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].
30238739	17	60	theme	fluorescein	2077:2087	arg1	staining					2109:2116	fluorescein diacetate live cell staining	2077:2116	fluorescein diacetate live cell staining	2077:2116	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	20	61	dep	higher	2671:2676	arg1	P<0.05					2721:2726	P<0.05	2721:2726	P<0.05	2721:2726	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	7	62	theme	Schwann	715:721	arg1	cells					723:727	Schwann cells	715:727	Schwann cells	715:727	Schwann cells were co-cultured in vitro with each group membranes to observe the biocompatibility by inverted microscope observing, living cell staining, cell counting, and immunofluorescence staining.
30238739	10	63	theme	nano	1222:1225	arg1	particles					1231:1239	nano PPy particles	1222:1239	nano PPy particles	1222:1239	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	8	64	theme	lysozyme	921:928	arg1	solution					930:937	The lysozyme solution	917:937	The lysozyme solution	917:937	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	23	65	theme	electrical	3013:3022	arg1	conductivity					3024:3035	electrical conductivity	3013:3035	electrical conductivity	3013:3035	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	2	66	theme	nano	200:203	arg1	PPy					205:207	The nano PPy	196:207	The nano PPy	196:207	Methods The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes.
30238739	17	67	theme	Schwann	2019:2025	arg1	cells					2027:2031	The cultured Schwann cells	2006:2031	The cultured Schwann cells	2006:2031	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	6	68	theme	group	695:699	arg1	membranes					677:685	membranes	677:685	membranes of each group	677:699	And the conductivity of membranes of each group was measured.
30238739	7	69	theme	cell	869:872	arg1	counting					874:881	cell counting	869:881	cell counting	869:881	Schwann cells were co-cultured in vitro with each group membranes to observe the biocompatibility by inverted microscope observing, living cell staining, cell counting, and immunofluorescence staining.
30238739	14	70	theme	×10	1644:1646	arg1	2±0.005					1634:1640	1.259 2±0.005 7	1628:1642	1.259 2±0.005 7	1628:1642	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	70	theme	×10	1644:1646	arg1	S/cm					1651:1654	(1.259 2±0.005 7)×10 -3 S/cm	1627:1654	(1.259 2±0.005 7)×10 -3 S/cm	1627:1654	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	71	from	conductivity	1667:1678	arg1	groups					1700:1705	groups B and C	1700:1713	groups B and C	1700:1713	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	71	from	conductivity	1667:1678	arg1	C					1713:1713	C	1713:1713	C	1713:1713	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	71	from	conductivity	1667:1678	arg1	B					1707:1707	B	1707:1707	B	1707:1707	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	15	72	theme	electron	1843:1850	arg1	microscope					1852:1861	scanning electron microscope	1834:1861	scanning electron microscope	1834:1861	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	10	73	theme	Scanning	1155:1162	arg1	observation					1184:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	12	74	theme	FT-IR	1395:1399	arg1	analysis					1401:1408	FT-IR analysis	1395:1408	FT-IR analysis of membranes in groups A and C	1395:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	8	75	from	degradation	964:974	arg1	vitro					996:1000	vitro	996:1000	vitro	996:1000	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	23	76	theme	PPy/chitin	2978:2987	arg1	membranes					2999:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	2	77	dep	Methods	188:194	arg1	formed					289:294	formed	289:294	formed membranes	289:304	Methods The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes.
30238739	2	77	dep	Methods	188:194	arg1	blended					258:264	blended	258:264	blended with chitosan	258:278	Methods The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes.
30238739	2	77	dep	Methods	188:194	arg1	synthesized					213:223	synthesized	213:223	synthesized by microemulsion polymerization	213:255	Methods The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes.
30238739	9	78	theme	458.4	1142:1146	arg1	cm					1148:1149	1 458.4 cm -1	1140:1152	1 458.4 cm -1	1140:1152	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	18	79	theme	Schwann	2254:2260	arg1	cells					2262:2266	Schwann cells	2254:2266	Schwann cells	2254:2266	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	8	80	used	used	943:946	arg2	solution					930:937	The lysozyme solution	917:937	The lysozyme solution	917:937	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	11	81	theme	PPy	1272:1274	arg1	particles					1276:1284	The nano PPy particles	1263:1284	The nano PPy particles	1263:1284	The nano PPy particles were synthesized.
30238739	5	82	theme	nano	611:614	arg1	PPy					616:618	the nano PPy	607:618	the nano PPy	607:618	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	13	83	theme	band	1482:1485	arg1	peaks					1461:1465	The characteristic peaks	1442:1465	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1	1442:1504	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after acetylated modification.
30238739	7	84	theme	inverted	816:823	arg1	microscope					825:834	inverted microscope	816:834	inverted microscope	816:834	Schwann cells were co-cultured in vitro with each group membranes to observe the biocompatibility by inverted microscope observing, living cell staining, cell counting, and immunofluorescence staining.
30238739	8	85	theme	membranes	983:991	arg1	degradation					964:974	the degradation	960:974	the degradation of the membranes in vitro	960:1000	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	19	86	theme	proliferation	2490:2502	arg1	ability					2466:2472	better ability	2459:2472	better ability of adhesion and proliferation	2459:2502	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	12	87	from	groups	1426:1431	arg1	analysis					1401:1408	FT-IR analysis	1395:1408	FT-IR analysis of membranes in groups A and C	1395:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	8	88	from	vitro	996:1000	arg1	degradation					964:974	the degradation	960:974	the degradation of the membranes in vitro	960:1000	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	17	89	theme	immunofluorescence	2139:2156	arg1	staining					2158:2165	soluble protein-100 immunofluorescence staining	2119:2165	soluble protein-100 immunofluorescence staining	2119:2165	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	15	90	theme	PPy	1742:1744	arg1	particles					1746:1754	The nano PPy particles	1733:1754	The nano PPy particles uniformly distributed on the surface of membranes in group A	1733:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	12	91	from	membranes	1413:1421	arg1	A					1433:1433	A	1433:1433	A	1433:1433	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	91	from	membranes	1413:1421	arg1	groups					1426:1431	groups A and C	1426:1439	groups A and C	1426:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	91	from	membranes	1413:1421	arg1	C					1439:1439	C	1439:1439	C	1439:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	23	92	contain	had	3009:3011	arg1	membranes					2999:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes	2973:3007	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	23	92	contain	had	3009:3011	arg2	degradability					3038:3050	degradability	3038:3050	degradability	3038:3050	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	23	92	contain	had	3009:3011	arg2	conductivity					3024:3035	electrical conductivity	3013:3035	electrical conductivity	3013:3035	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	23	92	contain	had	3009:3011	arg2	biocompatibility					3062:3077	good biocompatibility	3057:3077	good biocompatibility	3057:3077	Nano PPy/chitin composite membranes had electrical conductivity, degradability, and good biocompatibility in vitro.
30238739	15	93	from	A	1815:1815	arg1	surface					1785:1791	the surface	1781:1791	the surface of membranes in group A	1781:1815	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	20	94	theme	membranes	2624:2632	arg1	higher					2671:2676	higher	2671:2676	higher	2671:2676	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	20	94	theme	membranes	2624:2632	arg1	rates					2615:2619	the degradation rates	2599:2619	the degradation rates of membranes in groups A and C	2599:2650	The degradation of membranes in vitro showed that the degradation rates of membranes in groups A and C were significantly higher than those in group B at all time points ( P<0.05).
30238739	14	95	theme	group	1609:1613	arg1	A					1615:1615	group A	1609:1615	group A	1609:1615	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	13	96	theme	amide	1474:1478	arg1	band					1482:1485	the amide Ⅱ band	1470:1485	the amide Ⅱ band	1470:1485	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after acetylated modification.
30238739	1	97	theme	PPy	121:123	arg1	membrane					143:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	Objective To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.
30238739	12	98	from	analysis	1401:1408	arg1	A					1433:1433	A	1433:1433	A	1433:1433	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	98	from	analysis	1401:1408	arg1	groups					1426:1431	groups A and C	1426:1439	groups A and C	1426:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	98	from	analysis	1401:1408	arg1	C					1439:1439	C	1439:1439	C	1439:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	17	99	theme	inverted	2172:2179	arg1	observing					2192:2200	inverted microscope observing	2172:2200	inverted microscope observing	2172:2200	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	16	100	theme	PPy/chitin	1931:1940	arg1	membranes					1952:1960	the nano PPy/chitin composite membranes	1922:1960	the nano PPy/chitin composite membranes with electrical conductivity	1922:1989	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	16	100	theme	PPy/chitin	1931:1940	arg1	result					1914:1919	a result	1912:1919	a result	1912:1919	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	14	101	theme	membranes	1596:1604	arg1	conductivity					1580:1591	the conductivity	1576:1591	the conductivity of membranes in group A	1576:1615	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	5	102	theme	Scanning	538:545	arg1	microscopy					556:565	Scanning electron microscopy	538:565	Scanning electron microscopy	538:565	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	1	103	theme	/chitin	125:131	arg1	membrane					143:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	nano polypyrrole (PPy)/chitin composite membrane	103:150	Objective To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.
30238739	4	104	theme	chitosan	441:448	arg1	membranes					450:458	chitosan membranes	441:458	chitosan membranes (group B)	441:468	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	4	104	theme	chitosan	441:448	arg1	B					467:467	group B	461:467	group B	461:467	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	10	105	theme	microscopy	1173:1182	arg1	observation					1184:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation	1155:1194	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	13	106	theme	characteristic	1446:1459	arg1	peaks					1461:1465	The characteristic peaks	1442:1465	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1	1442:1504	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after acetylated modification.
30238739	4	107	theme	group	487:491	arg1	ones					481:484	chitin ones	474:484	chitin ones (group C)	474:494	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	4	107	theme	group	487:491	arg1	C					493:493	group C	487:493	group C	487:493	The chitosan membranes (group B) and chitin ones (group C) modified by acetylation acted as control.
30238739	19	108	theme	PPy/chitin	2424:2433	arg1	membranes					2445:2453	the nano PPy/chitin composite membranes	2415:2453	the nano PPy/chitin composite membranes	2415:2453	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	3	109	dep	membranes	379:387	arg1	membranes					416:424	nano PPy/chitin composite membranes	390:424	nano PPy/chitin composite membranes	390:424	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	109	dep	membranes	379:387	arg1	A					433:433	group A	427:433	group A	427:433	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	17	110	theme	live	2099:2102	arg1	staining					2109:2116	fluorescein diacetate live cell staining	2077:2116	fluorescein diacetate live cell staining	2077:2116	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	18	111	theme	control	2333:2339	arg1	groups					2341:2346	the two control groups	2325:2346	the two control groups	2325:2346	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	19	112	theme	adhesion	2477:2484	arg1	ability					2466:2472	better ability	2459:2472	better ability of adhesion and proliferation	2459:2502	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	16	113	theme	nano	1926:1929	arg1	membranes					1952:1960	the nano PPy/chitin composite membranes	1922:1960	the nano PPy/chitin composite membranes with electrical conductivity	1922:1989	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	16	113	theme	nano	1926:1929	arg1	result					1914:1919	a result	1912:1919	a result	1912:1919	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	6	114	theme	membranes	677:685	arg1	conductivity					661:672	the conductivity	657:672	the conductivity of membranes of each group	657:699	And the conductivity of membranes of each group was measured.
30238739	12	115	dep	groups	1426:1431	arg1	A					1433:1433	A	1433:1433	A	1433:1433	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	115	dep	groups	1426:1431	arg1	groups					1426:1431	groups A and C	1426:1439	groups A and C	1426:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	12	115	dep	groups	1426:1431	arg1	C					1439:1439	C	1439:1439	C	1439:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	19	116	theme	better	2459:2464	arg1	ability					2466:2472	better ability	2459:2472	better ability of adhesion and proliferation	2459:2502	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	18	117	dep	significant	2374:2384	arg1	P<0.05					2388:2393	P<0.05	2388:2393	P<0.05	2388:2393	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	18	117	dep	significant	2374:2384	arg1	significant					2374:2384	significant	2374:2384	significant	2374:2384	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	18	117	dep	significant	2374:2384	arg1	differences					2357:2367	the differences	2353:2367	the differences	2353:2367	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	17	118	theme	soluble	2119:2125	arg1	staining					2158:2165	soluble protein-100 immunofluorescence staining	2119:2165	soluble protein-100 immunofluorescence staining	2119:2165	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	16	119	theme	composite	1942:1950	arg1	membranes					1952:1960	the nano PPy/chitin composite membranes	1922:1960	the nano PPy/chitin composite membranes with electrical conductivity	1922:1989	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	16	119	theme	composite	1942:1950	arg1	result					1914:1919	a result	1912:1919	a result	1912:1919	As a result, the nano PPy/chitin composite membranes with electrical conductivity were obtained.
30238739	10	120	theme	PPy	1227:1229	arg1	particles					1231:1239	nano PPy particles	1222:1239	nano PPy particles	1222:1239	Scanning electron microscopy observation revealed that the size of nano PPy particles was about 100-200 nm.
30238739	2	121	theme	microemulsion	228:240	arg1	polymerization					242:255	microemulsion polymerization	228:255	microemulsion polymerization	228:255	Methods The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes.
30238739	3	122	theme	composite	406:414	arg1	membranes					416:424	nano PPy/chitin composite membranes	390:424	nano PPy/chitin composite membranes	390:424	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	122	theme	composite	406:414	arg1	A					433:433	group A	427:433	group A	427:433	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	17	123	theme	good	2060:2063	arg1	function					2065:2072	good function	2060:2072	good function	2060:2072	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	9	124	theme	absorption	1072:1081	arg1	peaks					1083:1087	the characteristic vibrational absorption peaks	1041:1087	the characteristic vibrational absorption peaks of C=C from nano PPy	1041:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	14	125	theme	membranes	1687:1695	arg1	conductivity					1667:1678	the conductivity	1663:1678	the conductivity of the membranes in groups B and C	1663:1713	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	5	126	theme	FT-IR	571:575	arg1	spectra					577:583	FT-IR spectra	571:583	FT-IR spectra	571:583	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	15	127	theme	control	1881:1887	arg1	groups					1889:1894	control groups	1881:1894	control groups	1881:1894	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	5	128	used	used	590:593	arg2	spectra					577:583	FT-IR spectra	571:583	FT-IR spectra	571:583	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	5	128	used	used	590:593	arg2	microscopy					556:565	Scanning electron microscopy	538:565	Scanning electron microscopy	538:565	Scanning electron microscopy and FT-IR spectra were used to identify the nano PPy and the membranes of each group.
30238739	3	129	theme	nano	390:393	arg1	membranes					416:424	nano PPy/chitin composite membranes	390:424	nano PPy/chitin composite membranes	390:424	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	3	129	theme	nano	390:393	arg1	A					433:433	group A	427:433	group A	427:433	The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).
30238739	18	130	theme	A	2301:2301	arg1	days					2276:2279	2 days	2274:2279	2 days	2274:2279	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	18	130	theme	A	2301:2301	arg1	days					2287:2290	4 days	2285:2290	4 days	2285:2290	The cell counting showed that the proliferation of Schwann cells after 2 days and 4 days of group A was more than that of the two control groups, and the differences were significant ( P<0.05).
30238739	21	131	theme	degradation	2745:2755	arg1	performance					2757:2767	the degradation performance	2741:2767	the degradation performance of the membranes modified by acetylation	2741:2808	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	21	131	theme	degradation	2745:2755	arg1	better					2814:2819	better	2814:2819	better	2814:2819	In a word, the degradation performance of the membranes modified by acetylation was better than that of chitosan membranes under the same condition.
30238739	0	132	theme	polypyrrole/chitin	42:59	arg1	membrane					71:78	nano polypyrrole/chitin composite membrane	37:78	nano polypyrrole/chitin composite membrane	37:78	[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].
30238739	19	133	theme	chitin	2531:2536	arg1	membranes					2538:2546	chitin membranes	2531:2546	chitin membranes	2531:2546	It indicated that the nano PPy/chitin composite membranes had better ability of adhesion and proliferation than those of chitosan and chitin membranes.
30238739	22	134	dep	nano	2894:2897	arg1	chitosan					2907:2914	chitosan	2907:2914	chitosan	2907:2914	Conclusion The nano PPy and chitosan can be blended and modified by acetylation successfully.
30238739	22	134	dep	nano	2894:2897	arg1	PPy					2899:2901	PPy	2899:2901	PPy	2899:2901	Conclusion The nano PPy and chitosan can be blended and modified by acetylation successfully.
30238739	0	135	theme	membrane	71:78	arg1	biocompatibility					17:32	biocompatibility	17:32	biocompatibility	17:32	[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].
30238739	0	135	theme	membrane	71:78	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation	0:11	[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].
30238739	14	136	dep	groups	1700:1705	arg1	groups					1700:1705	groups B and C	1700:1713	groups B and C	1700:1713	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	136	dep	groups	1700:1705	arg1	C					1713:1713	C	1713:1713	C	1713:1713	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	14	136	dep	groups	1700:1705	arg1	B					1707:1707	B	1707:1707	B	1707:1707	Conductivity test showed that the conductivity of membranes in group A was about (1.259 2±0.005 7)×10 -3 S/cm, while the conductivity of the membranes in groups B and C was not detected.
30238739	15	137	theme	scanning	1834:1841	arg1	microscope					1852:1861	scanning electron microscope	1834:1861	scanning electron microscope	1834:1861	The nano PPy particles uniformly distributed on the surface of membranes in group A were observed by scanning electron microscope; the membranes in control groups were smooth.
30238739	17	138	theme	cultured	2010:2017	arg1	cells					2027:2031	The cultured Schwann cells	2006:2031	The cultured Schwann cells	2006:2031	The cultured Schwann cells were found to survive with good function by fluorescein diacetate live cell staining, soluble protein-100 immunofluorescence staining, and inverted microscope observing.
30238739	9	139	theme	characteristic	1045:1058	arg1	peaks					1083:1087	the characteristic vibrational absorption peaks	1041:1087	the characteristic vibrational absorption peaks of C=C from nano PPy	1041:1108	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	12	140	theme	membranes	1413:1421	arg1	analysis					1401:1408	FT-IR analysis	1395:1408	FT-IR analysis of membranes in groups A and C	1395:1439	It was successful to turn chitosan to chitin by the acetylation, which was investigated by FT-IR analysis of membranes in groups A and C.
30238739	8	141	from	membranes	983:991	arg1	vitro					996:1000	vitro	996:1000	vitro	996:1000	The lysozyme solution was used to evaluate the degradation of the membranes in vitro.
30238739	9	142	theme	543.4	1124:1128	arg1	cm					1130:1131	1 543.4 cm -1	1122:1134	1 543.4 cm -1	1122:1134	Results The FT-IR spectra showed that the characteristic vibrational absorption peaks of C=C from nano PPy appeared at 1 543.4 cm -1 and 1 458.4 cm -1.
30238739	7	143	theme	cell	854:857	arg1	staining					859:866	cell staining	854:866	cell staining	854:866	Schwann cells were co-cultured in vitro with each group membranes to observe the biocompatibility by inverted microscope observing, living cell staining, cell counting, and immunofluorescence staining.
29655889	8	0	theme	lower	973:977	arg1	inhibition					986:995	lower growth inhibition	973:995	lower growth inhibition	973:995	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	7	1	theme	higher	931:936	arg1	leakage					942:948	higher LDH leakage	931:948	higher LDH leakage	931:948	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	5	2	theme	average	529:535	arg1	weights					547:553	the average molecular weights	525:553	the average molecular weights of 443.19kDa and 388.83kDa	525:580	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	9	3	theme	Flow	1068:1071	arg1	analysis					1084:1091	Flow cytometric analysis	1068:1091	Flow cytometric analysis	1068:1091	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	0	4	theme	polysaccharides	91:105	arg1	Berk					140:143	Berk	140:143	Berk	140:143	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	0	4	theme	polysaccharides	91:105	arg1	characterization					12:27	Composition characterization	0:27	Composition characterization	0:27	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	0	4	theme	polysaccharides	91:105	arg1	capacities					42:51	antioxidant capacities	30:51	antioxidant capacities	30:51	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	0	4	theme	polysaccharides	91:105	arg1	effects					76:82	anti-proliferative effects	57:82	anti-proliferative effects	57:82	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	10	5	dep	suggested	1181:1189	arg1	Berk					1303:1306	Berk	1303:1306	Berk	1303:1306	These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.)
29655889	8	6	theme	LDH	1044:1046	arg1	leakage					1048:1054	lower LDH leakage	1038:1054	lower LDH leakage	1038:1054	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	7	7	theme	human	886:890	arg1	cells					914:918	human hepatoblastoma HepG-2 cells	886:918	human hepatoblastoma HepG-2 cells	886:918	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	2	8	dep	designed	167:174	arg1	Berk					263:266	Berk	263:266	Berk	263:266	This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.)
29655889	0	9	theme	Trametes	121:128	arg1	lactinea					130:137	Trametes lactinea	121:137	Trametes lactinea	121:137	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	8	10	theme	L-02	1023:1026	arg1	cells					1028:1032	normal hepatocyte L-02 cells	1005:1032	normal hepatocyte L-02 cells	1005:1032	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	4	11	theme	Cellulose	376:384	arg1	DEAE-52					386:392	Cellulose DEAE-52	376:392	Cellulose DEAE-52	376:392	The crude TLP was fractionated into two fractions, namely TLP-1 and TLP-2 with Cellulose DEAE-52 and Sephadex G-150.
29655889	10	12	theme	anticancer	1268:1277	arg1	effects					1279:1285	the potential anticancer effects	1254:1285	the potential anticancer effects of T. lactinea	1254:1300	These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.)
29655889	6	13	theme	superoxide	737:746	arg1	radical					748:754	superoxide radical	737:754	superoxide radical	737:754	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	4	14	theme	Sephadex	398:405	arg1	G-150					407:411	Sephadex G-150	398:411	Sephadex G-150	398:411	The crude TLP was fractionated into two fractions, namely TLP-1 and TLP-2 with Cellulose DEAE-52 and Sephadex G-150.
29655889	7	15	theme	growth	860:865	arg1	inhibition					867:876	stronger growth inhibition	851:876	stronger growth inhibition	851:876	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	8	16	theme	normal	1005:1010	arg1	cells					1028:1032	normal hepatocyte L-02 cells	1005:1032	normal hepatocyte L-02 cells	1005:1032	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	11	17	theme	antioxidant	1352:1362	arg1	source					1364:1369	a natural antioxidant source	1342:1369	a natural antioxidant source applied in both healthy medicine and food industry for health benefits	1342:1440	Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
29655889	6	18	theme	NaCl	806:809	arg1	0.1M					798:801	0.1M	798:801	0.1M of NaCl	798:809	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	4	19	theme	crude	301:305	arg1	TLP					307:309	The crude TLP	297:309	The crude TLP	297:309	The crude TLP was fractionated into two fractions, namely TLP-1 and TLP-2 with Cellulose DEAE-52 and Sephadex G-150.
29655889	6	20	theme	water	608:612	arg1	elution					614:620	water elution	608:620	water elution	608:620	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	5	21	theme	388.83kDa	572:580	arg1	weights					547:553	the average molecular weights	525:553	the average molecular weights of 443.19kDa and 388.83kDa	525:580	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	6	22	theme	DPPH	722:725	arg1	radical					728:734	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	691:734	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	691:734	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	0	23	theme	Composition	0:10	arg1	Berk					140:143	Berk	140:143	Berk	140:143	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	0	23	theme	Composition	0:10	arg1	characterization					12:27	Composition characterization	0:27	Composition characterization	0:27	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	7	24	with	comparison	815:824	arg1	TLP-2					831:835	TLP-2	831:835	TLP-2	831:835	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	10	25	theme	lactinea	1293:1300	arg1	effects					1279:1285	the potential anticancer effects	1254:1285	the potential anticancer effects of T. lactinea	1254:1300	These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.)
29655889	0	26	theme	antioxidant	30:40	arg1	capacities					42:51	antioxidant capacities	30:51	antioxidant capacities	30:51	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	8	27	theme	lower	1038:1042	arg1	leakage					1048:1054	lower LDH leakage	1038:1054	lower LDH leakage	1038:1054	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	6	28	theme	1,1-diphenyl-2-picrylhydrazyl	691:719	arg1	radical					728:734	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	691:734	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	691:734	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	10	29	theme	potential	1258:1266	arg1	effects					1279:1285	the potential anticancer effects	1254:1285	the potential anticancer effects of T. lactinea	1254:1300	These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.)
29655889	11	30	theme	health	1426:1431	arg1	benefits					1433:1440	health benefits	1426:1440	health benefits	1426:1440	Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
29655889	3	31	theme	Pat	270:272	arg1	TLP					291:293	TLP	291:293	TLP	291:293	Pat polysaccharides (TLP).
29655889	3	31	theme	Pat	270:272	arg1	polysaccharides					274:288	Pat polysaccharides	270:288	Pat polysaccharides (TLP).	270:295	Pat polysaccharides (TLP).
29655889	9	32	theme	cytometric	1073:1082	arg1	analysis					1084:1091	Flow cytometric analysis	1068:1091	Flow cytometric analysis	1068:1091	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	2	33	theme	lactinea	253:260	arg1	characterization					204:219	characterization	204:219	characterization	204:219	This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.)
29655889	2	33	theme	lactinea	253:260	arg1	bioactivity					225:235	bioactivity	225:235	bioactivity	225:235	This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.)
29655889	9	34	theme	cells	1159:1163	arg1	apoptosis					1139:1147	apoptosis	1139:1147	apoptosis of HepG-2 cells	1139:1163	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	5	35	theme	FT-IR	423:427	arg1	analysis					429:436	FT-IR analysis	423:436	FT-IR analysis	423:436	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	8	36	theme	hepatocyte	1012:1021	arg1	cells					1028:1032	normal hepatocyte L-02 cells	1005:1032	normal hepatocyte L-02 cells	1005:1032	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	10	37	theme	T.	1290:1291	arg1	lactinea					1293:1300	T. lactinea	1290:1300	T. lactinea	1290:1300	These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.)
29655889	2	38	theme	Trametes	244:251	arg1	lactinea					253:260	the Trametes lactinea	240:260	the Trametes lactinea	240:260	This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.)
29655889	5	39	theme	molecular	537:545	arg1	weights					547:553	the average molecular weights	525:553	the average molecular weights of 443.19kDa and 388.83kDa	525:580	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	11	40	theme	food	1408:1411	arg1	industry					1413:1420	food industry	1408:1420	food industry	1408:1420	Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
29655889	11	41	theme	natural	1344:1350	arg1	source					1364:1369	a natural antioxidant source	1342:1369	a natural antioxidant source applied in both healthy medicine and food industry for health benefits	1342:1440	Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
29655889	0	42	theme	anti-proliferative	57:74	arg1	effects					76:82	anti-proliferative effects	57:82	anti-proliferative effects	57:82	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	9	43	theme	HepG-2	1152:1157	arg1	cells					1159:1163	HepG-2 cells	1152:1163	HepG-2 cells	1152:1163	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	6	44	theme	scavenging	661:670	arg1	activities					672:681	higher reducing power and scavenging activities	635:681	activities	672:681	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	5	45	theme	443.19kDa	558:566	arg1	weights					547:553	the average molecular weights	525:553	the average molecular weights of 443.19kDa and 388.83kDa	525:580	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	7	46	theme	LDH	938:940	arg1	leakage					942:948	higher LDH leakage	931:948	higher LDH leakage	931:948	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	0	47	attach	isolated	107:114	arg1	lactinea					130:137	Trametes lactinea	121:137	Trametes lactinea	121:137	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	0	47	attach	isolated	107:114	arg2	polysaccharides					91:105	the polysaccharides	87:105	the polysaccharides isolated from Trametes lactinea	87:137	Composition characterization, antioxidant capacities and anti-proliferative effects of the polysaccharides isolated from Trametes lactinea (Berk.)
29655889	5	48	with	heteropolysaccharides	471:491	arg1	weights					547:553	the average molecular weights	525:553	the average molecular weights of 443.19kDa and 388.83kDa	525:580	HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively.
29655889	6	49	from	elution	614:620	arg1	TLP-1					597:601	TLP-1	597:601	TLP-1 from water elution	597:620	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	6	50	theme	hydroxyl	760:767	arg1	radical					769:775	hydroxyl radical	760:775	hydroxyl radical	760:775	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	9	51	theme	stimulatory	1117:1127	arg1	effect					1129:1134	a stimulatory effect	1115:1134	a stimulatory effect	1115:1134	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	7	52	theme	hepatoblastoma	892:905	arg1	cells					914:918	human hepatoblastoma HepG-2 cells	886:918	human hepatoblastoma HepG-2 cells	886:918	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	7	53	theme	stronger	851:858	arg1	inhibition					867:876	stronger growth inhibition	851:876	stronger growth inhibition	851:876	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	11	54	theme	healthy	1387:1393	arg1	medicine					1395:1402	healthy medicine	1387:1402	healthy medicine	1387:1402	Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
29655889	2	55	theme	chemical	195:202	arg1	characterization					204:219	characterization	204:219	characterization	204:219	This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.)
29655889	7	56	theme	HepG-2	907:912	arg1	cells					914:918	human hepatoblastoma HepG-2 cells	886:918	human hepatoblastoma HepG-2 cells	886:918	In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage.
29655889	6	57	theme	reducing	642:649	arg1	power					651:655	higher reducing power and scavenging activities	635:681	power	651:655	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
29655889	9	58	contain	had	1111:1113	arg1	TLP-1					1105:1109	TLP-1	1105:1109	TLP-1	1105:1109	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	9	58	contain	had	1111:1113	arg2	effect					1129:1134	a stimulatory effect	1115:1134	a stimulatory effect	1115:1134	Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells.
29655889	8	59	theme	growth	979:984	arg1	inhibition					986:995	lower growth inhibition	973:995	lower growth inhibition	973:995	However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2.
29655889	6	60	theme	higher	635:640	arg1	power					651:655	higher reducing power and scavenging activities	635:681	power	651:655	TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl.
30861377	0	0	theme	O-specific	105:114	arg1	polysaccharide					116:129	the O-specific polysaccharide	101:129	the O-specific polysaccharide from Aeromonas hydrophila strain K691	101:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	2	1	theme	common	412:417	arg1	disaccharide					419:430	a common disaccharide	410:430	a common disaccharide in sequence	410:442	Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit.
30861377	1	2	theme	polysaccharide	287:300	arg1	unit					264:267	the pentasaccharide repeating unit	234:267	the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	234:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	3	theme	glycoside	201:209	arg1	form					176:179	the form	172:179	the form of its 2-aminoethyl glycoside	172:209	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	4	4	with	conjunction	731:741	arg1	N-iodosuccinimide					748:764	N-iodosuccinimide	748:764	N-iodosuccinimide	748:764	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	3	5	theme	glycconjugate	590:602	arg1	formation					604:612	further glycconjugate formation	582:612	further glycconjugate formation	582:612	The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation.
30861377	1	6	theme	Aeromonas	307:315	arg1	K691					334:337	Aeromonas hydrophilastrain K691	307:337	Aeromonas hydrophilastrain K691	307:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	7	theme	polysaccharide	116:129	arg1	unit					93:96	pentasaccharide repeating unit	67:96	pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691	67:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	3	8	theme	glycoside	549:557	arg1	form					524:527	the form	520:527	the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation	520:612	The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation.
30861377	1	9	theme	hydrophilastrain	317:332	arg1	K691					334:337	Aeromonas hydrophilastrain K691	307:337	Aeromonas hydrophilastrain K691	307:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	10	theme	hydrophila	146:155	arg1	strain					157:162	Aeromonas hydrophila strain K691	136:167	Aeromonas hydrophila strain K691	136:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	3	11	theme	target	491:496	arg1	structure					498:506	The target structure	487:506	The target structure	487:506	The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation.
30861377	0	12	theme	Aeromonas	136:144	arg1	strain					157:162	Aeromonas hydrophila strain K691	136:167	Aeromonas hydrophila strain K691	136:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	3	13	theme	further	582:588	arg1	formation					604:612	further glycconjugate formation	582:612	further glycconjugate formation	582:612	The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation.
30861377	1	14	from	K691	334:337	arg1	unit					264:267	the pentasaccharide repeating unit	234:267	the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	234:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	1	14	from	K691	334:337	arg1	polysaccharide					287:300	the O-specific polysaccharide	272:300	the O-specific polysaccharide from Aeromonas hydrophilastrain K691	272:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	2	15	theme	pentasaccharide	369:383	arg1	Synthesis					352:360	Synthesis	352:360	Synthesis of the pentasaccharide	352:383	Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit.
30861377	1	16	theme	Chemical	212:219	arg1	synthesis					221:229	Chemical synthesis	212:229	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	212:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	17	theme	Chemical	0:7	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose	0:54	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	4	18	theme	Stereoselective	615:629	arg1	glycosylations					631:644	Stereoselective glycosylations	615:644	Stereoselective glycosylations	615:644	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	2	19	from	disaccharide	419:430	arg1	sequence					435:442	sequence	435:442	sequence	435:442	Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit.
30861377	2	20	theme	sugar	475:479	arg1	unit					481:484	the rare sugar unit	466:484	the rare sugar unit	466:484	Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit.
30861377	2	21	theme	rare	470:473	arg1	unit					481:484	the rare sugar unit	466:484	the rare sugar unit	466:484	Synthesis of the pentasaccharide is accomplished by using a common disaccharide in sequence and finally attaching the rare sugar unit.
30861377	4	22	from	presence	703:710	arg1	conjunction					731:741	conjunction	731:741	conjunction with N-iodosuccinimide	731:764	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	1	23	theme	pentasaccharide	238:252	arg1	unit					264:267	the pentasaccharide repeating unit	234:267	the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	234:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	24	theme	4-amino-4,6-dideoxy-d-glucose	26:54	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose	0:54	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	1	25	theme	repeating	254:262	arg1	unit					264:267	the pentasaccharide repeating unit	234:267	the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	234:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	26	theme	repeating	83:91	arg1	unit					93:96	pentasaccharide repeating unit	67:96	pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691	67:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	0	27	from	unit	93:96	arg1	strain					157:162	Aeromonas hydrophila strain K691	136:167	Aeromonas hydrophila strain K691	136:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	1	28	from	unit	264:267	arg1	K691					334:337	Aeromonas hydrophilastrain K691	307:337	Aeromonas hydrophilastrain K691	307:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	0	29	theme	pentasaccharide	67:81	arg1	unit					93:96	pentasaccharide repeating unit	67:96	pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691	67:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	4	30	attach	presence	703:710	arg1	conjunction					731:741	conjunction	731:741	conjunction with N-iodosuccinimide	731:764	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	4	30	attach	presence	703:710	arg2	H2SO4-silica					715:726	H2SO4-silica	715:726	H2SO4-silica	715:726	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	4	31	gly	glycosylations	631:644	arg1	presence					703:710	the presence	699:710	the presence of H2SO4-silica in conjunction with N-iodosuccinimide	699:764	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	3	32	theme	2-aminoethyl	536:547	arg1	glycoside					549:557	its 2-aminoethyl glycoside	532:557	its 2-aminoethyl glycoside which is essential for further glycconjugate formation	532:612	The target structure was made in the form of its 2-aminoethyl glycoside which is essential for further glycconjugate formation.
30861377	0	33	theme	2-aminoethyl	188:199	arg1	glycoside					201:209	its 2-aminoethyl glycoside	184:209	its 2-aminoethyl glycoside	184:209	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	1	34	theme	unit	264:267	arg1	synthesis					221:229	Chemical synthesis	212:229	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691	212:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
30861377	4	35	theme	H2SO4-silica	715:726	arg1	presence					703:710	the presence	699:710	the presence of H2SO4-silica in conjunction with N-iodosuccinimide	699:764	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	0	36	from	strain	157:162	arg1	unit					93:96	pentasaccharide repeating unit	67:96	pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691	67:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	0	36	from	strain	157:162	arg1	polysaccharide					116:129	the O-specific polysaccharide	101:129	the O-specific polysaccharide from Aeromonas hydrophila strain K691	101:167	Chemical synthesis of the 4-amino-4,6-dideoxy-d-glucose containing pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophila strain K691 in the form of its 2-aminoethyl glycoside.
30861377	4	37	theme	thioglycosides	681:694	arg1	activation					667:676	the activation	663:676	the activation of thioglycosides	663:694	Stereoselective glycosylations were achieved by the activation of thioglycosides in the presence of H2SO4-silica in conjunction with N-iodosuccinimide.
30861377	1	38	theme	O-specific	276:285	arg1	polysaccharide					287:300	the O-specific polysaccharide	272:300	the O-specific polysaccharide from Aeromonas hydrophilastrain K691	272:337	Chemical synthesis of the pentasaccharide repeating unit of the O-specific polysaccharide from Aeromonas hydrophilastrain K691 is reported.
29803006	12	0	theme	SIGNIFICANCE	1968:1979	arg1	STATEMENT					1955:1963	STATEMENT	1955:1963	STATEMENT OF SIGNIFICANCE Although adhesiolysis	1955:2001	STATEMENT OF SIGNIFICANCE Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	14	1	theme	composite	2425:2433	arg1	hydrogel					2435:2442	a thermoresponsive polysaccharide-based composite hydrogel	2385:2442	a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	2385:2527	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	14	2	theme	thermoresponsive	2387:2402	arg1	hydrogel					2435:2442	a thermoresponsive polysaccharide-based composite hydrogel	2385:2442	a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	2385:2527	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	1	3	theme	Postoperative	163:175	arg1	complications					203:215	very common complications	191:215	very common complications after general abdominal surgery	191:247	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	1	3	theme	Postoperative	163:175	arg1	adhesions					177:185	Postoperative adhesions	163:185	Postoperative adhesions	163:185	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	4	4	theme	driving	929:935	arg1	factors					937:943	extra driving factors	923:943	extra driving factors	923:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	2	5	theme	new	344:346	arg1	trauma					348:353	the new trauma	340:353	the new trauma caused by surgical lysis	340:378	Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	9	6	theme	rigorous	1570:1577	arg1	model					1608:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	4	7	theme	barrier	738:744	arg1	hydrogel					632:639	a thermoresponsive hydrogel	613:639	a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	613:717	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	4	7	theme	barrier	738:744	arg1	device					746:751	a barrier device	736:751	a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors	736:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	9	8	theme	repeated-injury	1583:1597	arg1	model					1608:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	16	9	theme	recurrent	2809:2817	arg1	adhesion					2819:2826	recurrent adhesion	2809:2826	recurrent adhesion	2809:2826	Our study provide an effective anti-adhesion system for preventing recurrent adhesion after adhesiolysis.
29803006	14	10	theme	present	2357:2363	arg1	study					2365:2369	the present study	2353:2369	the present study	2353:2369	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	2	11	theme	surgical	365:372	arg1	lysis					374:378	surgical lysis	365:378	surgical lysis	365:378	Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	6	12	theme	Rheological	1052:1062	arg1	measurements					1064:1075	Rheological measurements	1052:1075	Rheological measurements	1052:1075	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	13	13	theme	adhesion	2321:2328	arg1	prevention					2297:2306	prevention	2297:2306	prevention of recurrent adhesion	2297:2328	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	8	14	theme	skin	1432:1435	arg1	model					1443:1447	the rat skin wound model	1424:1447	the rat skin wound model	1424:1447	Furthermore, the mXG/HBC hydrogel could promote wound healing in the rat skin wound model.
29803006	11	15	theme	anti-adhesion	1907:1919	arg1	system					1921:1926	an injectable anti-adhesion system	1893:1926	an injectable anti-adhesion system for clinical applications	1893:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	15	16	theme	mXG/HBC	2544:2550	arg1	hydrogel					2562:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	15	16	theme	mXG/HBC	2544:2550	arg1	easy					2584:2587	easy	2584:2587	easy	2584:2587	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	5	17	theme	etherification	1013:1026	arg1	reaction					1028:1035	etherification reaction	1013:1035	etherification reaction	1013:1035	First, mXG and HBC were synthesized via enzymatic modification and etherification reaction, respectively.
29803006	6	18	theme	thermosensitivity	1138:1154	arg1	properties					1156:1165	excellent thermosensitivity properties	1128:1165	excellent thermosensitivity properties	1128:1165	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	2	19	theme	preexisting	317:327	arg1	adhesions					329:337	the preexisting adhesions	313:337	the preexisting adhesions	313:337	Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	4	20	theme	adhesion	767:774	arg1	prevention					776:785	recurrent adhesion prevention	757:785	recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors	757:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	13	21	theme	few	2271:2273	arg1	attention					2275:2283	few attention	2271:2283	few attention	2271:2283	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	12	22	theme	surgical	2096:2103	arg1	lysis					2105:2109	surgical lysis	2096:2109	surgical lysis	2096:2109	STATEMENT OF SIGNIFICANCE Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	1	23	theme	general	223:229	arg1	surgery					241:247	general abdominal surgery	223:247	general abdominal surgery	223:247	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	6	24	theme	composite	1104:1112	arg1	system					1114:1119	the mXG/HBC composite system	1092:1119	the mXG/HBC composite system	1092:1119	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	3	25	theme	recurrent	429:437	arg1	adhesion					439:446	recurrent adhesion	429:446	recurrent adhesion	429:446	The prevention of recurrent adhesion after adhesiolysis is more difficult because the injury is more severe and adhesion mechanism is more complicated compared with the primary adhesion.
29803006	4	26	theme	gelling	847:853	arg1	behaviors					855:863	spontaneous gelling behaviors	835:863	spontaneous gelling behaviors	835:863	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	14	27	theme	hydroxybutyl	2501:2512	arg1	HBC					2524:2526	HBC	2524:2526	HBC	2524:2526	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	14	27	theme	hydroxybutyl	2501:2512	arg1	chitosan					2514:2521	hydroxybutyl chitosan	2501:2521	hydroxybutyl chitosan (HBC)	2501:2527	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	15	28	theme	scare	2725:2729	arg1	formation					2731:2739	scare formation	2725:2739	scare formation	2725:2739	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	13	29	theme	primary	2237:2243	arg1	prevention					2254:2263	primary adhesion prevention	2237:2263	primary adhesion prevention	2237:2263	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	12	30	theme	new	2075:2077	arg1	trauma					2079:2084	the new trauma	2071:2084	the new trauma caused by surgical lysis	2071:2109	STATEMENT OF SIGNIFICANCE Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	0	31	theme	Thermoresponsive	0:15	arg1	hydrogel					48:55	Thermoresponsive polysaccharide-based composite hydrogel	0:55	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.	0:161	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	15	32	theme	wound	2700:2704	arg1	healing					2706:2712	wound healing	2700:2712	wound healing	2700:2712	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	4	33	theme	thermoresponsive	615:630	arg1	hydrogel					632:639	a thermoresponsive hydrogel	613:639	a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	613:717	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	4	33	theme	thermoresponsive	615:630	arg1	device					746:751	a barrier device	736:751	a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors	736:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	0	34	theme	composite	38:46	arg1	hydrogel					48:55	Thermoresponsive polysaccharide-based composite hydrogel	0:55	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.	0:161	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	12	35	theme	preexisting	2048:2058	arg1	adhesions					2060:2068	the preexisting adhesions	2044:2068	the preexisting adhesions	2044:2068	STATEMENT OF SIGNIFICANCE Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	4	36	theme	chemical	901:908	arg1	reactions					910:918	any chemical reactions	897:918	any chemical reactions	897:918	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	3	37	theme	adhesion	523:530	arg1	mechanism					532:540	adhesion mechanism	523:540	adhesion mechanism	523:540	The prevention of recurrent adhesion after adhesiolysis is more difficult because the injury is more severe and adhesion mechanism is more complicated compared with the primary adhesion.
29803006	7	38	theme	mXG/HBC	1272:1278	arg1	hydrogel					1280:1287	the mXG/HBC hydrogel	1268:1287	the mXG/HBC hydrogel	1268:1287	Moreover, the mXG/HBC hydrogel exhibited excellent cytocompatibility and hemocompatibility in vitro.
29803006	9	39	theme	composite	1487:1495	arg1	hydrogel					1497:1504	the mXG/HBC composite hydrogel	1475:1504	the mXG/HBC composite hydrogel	1475:1504	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	16	40	theme	effective	2763:2771	arg1	system					2787:2792	an effective anti-adhesion system	2760:2792	an effective anti-adhesion system for preventing recurrent adhesion after adhesiolysis	2760:2845	Our study provide an effective anti-adhesion system for preventing recurrent adhesion after adhesiolysis.
29803006	13	41	theme	pharmacological	2191:2205	arg1	approaches					2207:2216	pharmacological approaches	2191:2216	pharmacological approaches	2191:2216	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	4	42	theme	body	872:875	arg1	temperature					877:887	the body temperature	868:887	the body temperature without any chemical reactions or extra driving factors	868:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	14	43	theme	simple	2447:2452	arg1	mixing					2454:2459	simple mixing	2447:2459	simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	2447:2527	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	11	44	theme	promising	1870:1878	arg1	candidate					1880:1888	a promising candidate	1868:1888	a promising candidate as an injectable anti-adhesion system for clinical applications	1868:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	11	44	theme	promising	1870:1878	arg1	hydrogel					1852:1859	the mXG/HBC composite hydrogel	1830:1859	the mXG/HBC composite hydrogel	1830:1859	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	13	45	theme	existing	2162:2169	arg1	systems					2179:2185	the existing barrier systems	2158:2185	the existing barrier systems	2158:2185	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	9	46	theme	recurrent	1527:1535	arg1	adhesion					1537:1544	recurrent adhesion	1527:1544	recurrent adhesion	1527:1544	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	4	47	theme	hydroxybutyl	691:702	arg1	HBC					714:716	HBC	714:716	HBC	714:716	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	4	47	theme	hydroxybutyl	691:702	arg1	chitosan					704:711	hydroxybutyl chitosan	691:711	hydroxybutyl chitosan (HBC)	691:717	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	3	48	theme	primary	580:586	arg1	adhesion					588:595	the primary adhesion	576:595	the primary adhesion	576:595	The prevention of recurrent adhesion after adhesiolysis is more difficult because the injury is more severe and adhesion mechanism is more complicated compared with the primary adhesion.
29803006	4	49	theme	modified	661:668	arg1	mXG					682:684	mXG	682:684	mXG	682:684	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	4	49	theme	modified	661:668	arg1	xyloglucan					670:679	galactose modified xyloglucan	651:679	galactose modified xyloglucan (mXG)	651:685	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	14	50	theme	polysaccharide-based	2404:2423	arg1	hydrogel					2435:2442	a thermoresponsive polysaccharide-based composite hydrogel	2385:2442	a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC)	2385:2527	In the present study, we developed a thermoresponsive polysaccharide-based composite hydrogel by simple mixing galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC).
29803006	11	51	theme	mXG/HBC	1834:1840	arg1	candidate					1880:1888	a promising candidate	1868:1888	a promising candidate as an injectable anti-adhesion system for clinical applications	1868:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	11	51	theme	mXG/HBC	1834:1840	arg1	hydrogel					1852:1859	the mXG/HBC composite hydrogel	1830:1859	the mXG/HBC composite hydrogel	1830:1859	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	1	52	theme	abdominal	231:239	arg1	surgery					241:247	general abdominal surgery	223:247	general abdominal surgery	223:247	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	4	53	theme	extra	923:927	arg1	factors					937:943	extra driving factors	923:943	extra driving factors	923:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	8	54	theme	mXG/HBC	1376:1382	arg1	hydrogel					1384:1391	the mXG/HBC hydrogel	1372:1391	the mXG/HBC hydrogel	1372:1391	Furthermore, the mXG/HBC hydrogel could promote wound healing in the rat skin wound model.
29803006	10	55	theme	wound	1759:1763	arg1	healing					1765:1771	wound healing	1759:1771	wound healing	1759:1771	The results demonstrated that the composite hydrogel could not only effectively prevent recurrent adhesion after adhesiolysis, but also promote wound healing and reduce scare formation.
29803006	9	56	theme	rat	1579:1581	arg1	model					1608:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	9	57	theme	adhesion	1599:1606	arg1	model					1608:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	a more rigorous rat repeated-injury adhesion model	1563:1612	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	8	58	theme	wound	1407:1411	arg1	healing					1413:1419	wound healing	1407:1419	wound healing	1407:1419	Furthermore, the mXG/HBC hydrogel could promote wound healing in the rat skin wound model.
29803006	10	59	theme	scare	1784:1788	arg1	formation					1790:1798	scare formation	1784:1798	scare formation	1784:1798	The results demonstrated that the composite hydrogel could not only effectively prevent recurrent adhesion after adhesiolysis, but also promote wound healing and reduce scare formation.
29803006	13	60	theme	recurrent	2311:2319	arg1	adhesion					2321:2328	recurrent adhesion	2311:2328	recurrent adhesion	2311:2328	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	12	61	theme	recurrent	2122:2130	arg1	adhesion					2132:2139	recurrent adhesion	2122:2139	recurrent adhesion	2122:2139	STATEMENT OF SIGNIFICANCE Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	16	62	theme	anti-adhesion	2773:2785	arg1	system					2787:2792	an effective anti-adhesion system	2760:2792	an effective anti-adhesion system for preventing recurrent adhesion after adhesiolysis	2760:2845	Our study provide an effective anti-adhesion system for preventing recurrent adhesion after adhesiolysis.
29803006	5	63	theme	enzymatic	986:994	arg1	modification					996:1007	enzymatic modification	986:1007	enzymatic modification	986:1007	First, mXG and HBC were synthesized via enzymatic modification and etherification reaction, respectively.
29803006	8	64	theme	rat	1428:1430	arg1	model					1443:1447	the rat skin wound model	1424:1447	the rat skin wound model	1424:1447	Furthermore, the mXG/HBC hydrogel could promote wound healing in the rat skin wound model.
29803006	8	65	theme	wound	1437:1441	arg1	model					1443:1447	the rat skin wound model	1424:1447	the rat skin wound model	1424:1447	Furthermore, the mXG/HBC hydrogel could promote wound healing in the rat skin wound model.
29803006	15	66	theme	composite	2552:2560	arg1	hydrogel					2562:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	15	66	theme	composite	2552:2560	arg1	easy					2584:2587	easy	2584:2587	easy	2584:2587	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	4	67	from	temperature	877:887	arg1	injectability					817:829	its injectability	813:829	its injectability	813:829	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	4	67	from	temperature	877:887	arg1	behaviors					855:863	spontaneous gelling behaviors	835:863	spontaneous gelling behaviors	835:863	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	1	68	theme	common	196:201	arg1	complications					203:215	very common complications	191:215	very common complications after general abdominal surgery	191:247	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	1	68	theme	common	196:201	arg1	adhesions					177:185	Postoperative adhesions	163:185	Postoperative adhesions	163:185	Postoperative adhesions are very common complications after general abdominal surgery.
29803006	6	69	theme	excellent	1128:1136	arg1	properties					1156:1165	excellent thermosensitivity properties	1128:1165	excellent thermosensitivity properties	1128:1165	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	11	70	theme	injectable	1896:1905	arg1	system					1921:1926	an injectable anti-adhesion system	1893:1926	an injectable anti-adhesion system for clinical applications	1893:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	0	71	theme	recurrent	124:132	arg1	adhesion					134:141	recurrent adhesion	124:141	recurrent adhesion	124:141	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	15	72	theme	recurrent	2638:2646	arg1	adhesion					2648:2655	the recurrent adhesion	2634:2655	the recurrent adhesion	2634:2655	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	4	73	theme	recurrent	757:765	arg1	prevention					776:785	recurrent adhesion prevention	757:785	recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors	757:943	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	6	74	theme	mXG/HBC	1096:1102	arg1	system					1114:1119	the mXG/HBC composite system	1092:1119	the mXG/HBC composite system	1092:1119	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	3	75	theme	adhesion	439:446	arg1	difficult					475:483	difficult	475:483	difficult	475:483	The prevention of recurrent adhesion after adhesiolysis is more difficult because the injury is more severe and adhesion mechanism is more complicated compared with the primary adhesion.
29803006	3	75	theme	adhesion	439:446	arg1	prevention					415:424	The prevention	411:424	The prevention of recurrent adhesion after adhesiolysis	411:465	The prevention of recurrent adhesion after adhesiolysis is more difficult because the injury is more severe and adhesion mechanism is more complicated compared with the primary adhesion.
29803006	4	76	theme	spontaneous	835:845	arg1	behaviors					855:863	spontaneous gelling behaviors	835:863	spontaneous gelling behaviors	835:863	In this study, a thermoresponsive hydrogel contained galactose modified xyloglucan (mXG) and hydroxybutyl chitosan (HBC) was developed as a barrier device for recurrent adhesion prevention after adhesiolysis due to its injectability and spontaneous gelling behaviors at the body temperature without any chemical reactions or extra driving factors.
29803006	11	77	theme	clinical	1932:1939	arg1	applications					1941:1952	clinical applications	1932:1952	clinical applications	1932:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	0	78	theme	polysaccharide-based	17:36	arg1	hydrogel					48:55	Thermoresponsive polysaccharide-based composite hydrogel	0:55	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.	0:161	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	13	79	theme	adhesion	2245:2252	arg1	prevention					2254:2263	primary adhesion prevention	2237:2263	primary adhesion prevention	2237:2263	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	9	80	from	efficacy	1463:1470	arg1	prevention					1513:1522	the prevention	1509:1522	the prevention of recurrent adhesion	1509:1544	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	10	81	theme	recurrent	1703:1711	arg1	adhesion					1713:1720	recurrent adhesion	1703:1720	recurrent adhesion	1703:1720	The results demonstrated that the composite hydrogel could not only effectively prevent recurrent adhesion after adhesiolysis, but also promote wound healing and reduce scare formation.
29803006	6	82	theme	gelation	1178:1185	arg1	temperature					1187:1197	their gelation temperature	1172:1197	their gelation temperature	1172:1197	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	0	83	theme	antibacterial	62:74	arg1	activities					98:107	antibacterial and healing-promoting activities	62:107	antibacterial and healing-promoting activities	62:107	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	9	84	theme	mXG/HBC	1479:1485	arg1	hydrogel					1497:1504	the mXG/HBC composite hydrogel	1475:1504	the mXG/HBC composite hydrogel	1475:1504	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	11	85	theme	composite	1842:1850	arg1	candidate					1880:1888	a promising candidate	1868:1888	a promising candidate as an injectable anti-adhesion system for clinical applications	1868:1952	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	11	85	theme	composite	1842:1850	arg1	hydrogel					1852:1859	the mXG/HBC composite hydrogel	1830:1859	the mXG/HBC composite hydrogel	1830:1859	These results suggested that the mXG/HBC composite hydrogel may be a promising candidate as an injectable anti-adhesion system for clinical applications.
29803006	0	86	with	hydrogel	48:55	arg1	activities					98:107	antibacterial and healing-promoting activities	62:107	antibacterial and healing-promoting activities	62:107	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	0	87	theme	healing-promoting	80:96	arg1	activities					98:107	antibacterial and healing-promoting activities	62:107	antibacterial and healing-promoting activities	62:107	Thermoresponsive polysaccharide-based composite hydrogel with antibacterial and healing-promoting activities for preventing recurrent adhesion after adhesiolysis.
29803006	9	88	theme	hydrogel	1497:1504	arg1	efficacy					1463:1470	the efficacy	1459:1470	the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion	1459:1544	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
29803006	6	89	theme	mXG/HBC	1243:1249	arg1	ratio					1251:1255	the mXG/HBC ratio	1239:1255	the mXG/HBC ratio	1239:1255	Rheological measurements indicated that the mXG/HBC composite system showed excellent thermosensitivity properties, and their gelation temperature and time can be modulated via adjusting the mXG/HBC ratio.
29803006	10	90	theme	composite	1649:1657	arg1	hydrogel					1659:1666	the composite hydrogel	1645:1666	the composite hydrogel	1645:1666	The results demonstrated that the composite hydrogel could not only effectively prevent recurrent adhesion after adhesiolysis, but also promote wound healing and reduce scare formation.
29803006	2	91	theme	recurrent	391:399	arg1	adhesion					401:408	recurrent adhesion	391:408	recurrent adhesion	391:408	Although adhesiolysis has been proven effective in eliminating the preexisting adhesions, the new trauma caused by surgical lysis can induce recurrent adhesion.
29803006	15	92	theme	resulting	2534:2542	arg1	hydrogel					2562:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel	2530:2569	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	15	92	theme	resulting	2534:2542	arg1	easy					2584:2587	easy	2584:2587	easy	2584:2587	The resulting mXG/HBC composite hydrogel not only was easy to handle and highly effective in preventing the recurrent adhesion after adhesiolysis, but also could promote wound healing and reduce scare formation.
29803006	13	93	theme	barrier	2171:2177	arg1	systems					2179:2185	the existing barrier systems	2158:2185	the existing barrier systems	2158:2185	So far, most of the existing barrier systems and pharmacological approaches were developed for primary adhesion prevention while few attention has paid on prevention of recurrent adhesion after adhesiolysis.
29803006	7	94	theme	excellent	1299:1307	arg1	cytocompatibility					1309:1325	excellent cytocompatibility	1299:1325	excellent cytocompatibility	1299:1325	Moreover, the mXG/HBC hydrogel exhibited excellent cytocompatibility and hemocompatibility in vitro.
29803006	9	95	theme	adhesion	1537:1544	arg1	prevention					1513:1522	the prevention	1509:1522	the prevention of recurrent adhesion	1509:1544	Finally, the efficacy of the mXG/HBC composite hydrogel in the prevention of recurrent adhesion was evaluated in a more rigorous rat repeated-injury adhesion model.
31536333	1	0	theme	co-precipitation	304:319	arg1	method					321:326	co-precipitation method	304:326	co-precipitation method	304:326	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	1	1	theme	regenerated	217:227	arg1	RC-SP					254:258	RC-SP	254:258	RC-SP	254:258	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	1	1	theme	regenerated	217:227	arg1	pulp					248:251	regenerated cellulose-softwood pulp	217:251	regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement	217:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	0	2	theme	vinyl	69:73	arg1	Poly					64:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly	0:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)	0:82	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	0	2	theme	vinyl	69:73	arg1	alcohol					75:81	vinyl alcohol	69:81	vinyl alcohol	69:81	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	2	3	theme	Simultaneous	329:340	arg1	precipitation					342:354	Simultaneous precipitation	329:354	Simultaneous precipitation of the two components	329:376	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	1	4	theme	cellulose-softwood	229:246	arg1	RC-SP					254:258	RC-SP	254:258	RC-SP	254:258	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	1	4	theme	cellulose-softwood	229:246	arg1	pulp					248:251	regenerated cellulose-softwood pulp	217:251	regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement	217:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	5	5	theme	RC-SP	1018:1022	arg1	composite					1035:1043	10% RC-SP reinforced composite	1014:1043	10% RC-SP reinforced composite	1014:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	6	6	theme	renewable	1198:1206	arg1	resources					1218:1226	renewable cellulose resources	1198:1226	renewable cellulose resources	1198:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	4	7	theme	dynamic	790:796	arg1	properties					809:818	dynamic mechanical properties	790:818	dynamic mechanical properties	790:818	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	5	8	theme	shape	991:995	arg1	rate					1006:1009	shape recovery rate	991:1009	shape recovery rate of 10% RC-SP reinforced composite	991:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	6	9	theme	resources	1218:1226	arg1	application					1183:1193	the application	1179:1193	the application of renewable cellulose resources	1179:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	6	9	theme	resources	1218:1226	arg1	method					1168:1173	co-precipitation method	1151:1173	co-precipitation method	1151:1173	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	5	10	theme	memory	972:977	arg1	effect					979:984	good water-induced shape memory effect	947:984	good water-induced shape memory effect	947:984	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	5	11	theme	good	947:950	arg1	effect					979:984	good water-induced shape memory effect	947:984	good water-induced shape memory effect	947:984	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	3	12	theme	cross-linking	537:549	arg1	network					551:557	This physical cross-linking network	523:557	This physical cross-linking network	523:557	This physical cross-linking network reduces the water absorption and improves the water resistance of the composites.
31536333	2	13	theme	components	367:376	arg1	precipitation					342:354	Simultaneous precipitation	329:354	Simultaneous precipitation of the two components	329:376	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	2	14	theme	uniform	387:393	arg1	dispersion					395:404	uniform dispersion	387:404	uniform dispersion of the RC-SP	387:417	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	0	15	theme	Cellulose	96:104	arg1	Biocomposites					106:118	Cellulose Biocomposites	96:118	Cellulose Biocomposites	96:118	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	4	16	theme	mechanical	798:807	arg1	properties					809:818	dynamic mechanical properties	790:818	dynamic mechanical properties	790:818	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	6	17	theme	useful	1106:1111	arg1	information					1113:1123	useful information	1106:1123	useful information for the implementation of co-precipitation method and the application of renewable cellulose resources	1106:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	0	18	theme	Strong	13:18	arg1	Poly					64:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly	0:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)	0:82	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	0	18	theme	Strong	13:18	arg1	alcohol					75:81	vinyl alcohol	69:81	vinyl alcohol	69:81	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	2	19	theme	bonding	484:490	arg1	network					492:498	hydrogen bonding network	475:498	hydrogen bonding network	475:498	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	1	20	theme	poly	172:175	arg1	biocomposites					198:210	poly(vinyl alcohol) (PVA) biocomposites	172:210	poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement	172:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	2	21	theme	strong	434:439	arg1	entanglements					457:469	strong molecular chain entanglements	434:469	strong molecular chain entanglements	434:469	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	6	22	theme	application	1183:1193	arg1	implementation					1133:1146	the implementation	1129:1146	the implementation of co-precipitation method and the application of renewable cellulose resources	1129:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	0	23	theme	Shape	51:55	arg1	Poly					64:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly	0:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)	0:82	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	0	23	theme	Shape	51:55	arg1	alcohol					75:81	vinyl alcohol	69:81	vinyl alcohol	69:81	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	5	24	theme	reinforced	1024:1033	arg1	composite					1035:1043	10% RC-SP reinforced composite	1014:1043	10% RC-SP reinforced composite	1014:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	3	25	theme	water	571:575	arg1	absorption					577:586	the water absorption	567:586	the water absorption	567:586	This physical cross-linking network reduces the water absorption and improves the water resistance of the composites.
31536333	2	26	theme	RC-SP	413:417	arg1	dispersion					395:404	uniform dispersion	387:404	uniform dispersion of the RC-SP	387:417	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	5	27	theme	water-induced	952:964	arg1	effect					979:984	good water-induced shape memory effect	947:984	good water-induced shape memory effect	947:984	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	6	28	theme	cellulose	1208:1216	arg1	resources					1218:1226	renewable cellulose resources	1198:1226	renewable cellulose resources	1198:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	4	29	theme	mechanical	764:773	arg1	properties					775:784	mechanical properties	764:784	mechanical properties	764:784	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	5	30	theme	fabricated	907:916	arg1	composites					928:937	the fabricated PVA/RC-SP composites	903:937	the fabricated PVA/RC-SP composites	903:937	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	2	31	theme	hydrogen	475:482	arg1	network					492:498	hydrogen bonding network	475:498	hydrogen bonding network	475:498	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	4	32	theme	thermal	690:696	arg1	properties					712:721	the thermal decomposition properties	686:721	the thermal decomposition properties of the composites	686:739	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	0	33	theme	Co-precipitation	133:148	arg1	Method					150:155	a Facile Co-precipitation Method	124:155	a Facile Co-precipitation Method	124:155	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	6	34	theme	co-precipitation	1151:1166	arg1	method					1168:1173	co-precipitation method	1151:1173	co-precipitation method	1151:1173	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	1	35	theme	green	266:270	arg1	reinforcement					272:284	a green reinforcement	264:284	a green reinforcement	264:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	0	36	theme	Facile	126:131	arg1	Method					150:155	a Facile Co-precipitation Method	124:155	a Facile Co-precipitation Method	124:155	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	4	37	theme	strong	839:844	arg1	interaction					846:856	the strong interaction	835:856	the strong interaction between the filler and the matrix	835:890	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	4	38	theme	RC-SP	662:666	arg1	incorporation					645:657	The incorporation	641:657	The incorporation of RC-SP	641:666	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	0	39	theme	Memory	57:62	arg1	Poly					64:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly	0:67	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)	0:82	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	0	39	theme	Memory	57:62	arg1	alcohol					75:81	vinyl alcohol	69:81	vinyl alcohol	69:81	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	4	40	dep	properties	775:784	arg1	the					760:762	the	760:762	the	760:762	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	2	41	theme	chain	451:455	arg1	entanglements					457:469	strong molecular chain entanglements	434:469	strong molecular chain entanglements	434:469	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31536333	3	42	theme	water	605:609	arg1	resistance					611:620	the water resistance	601:620	the water resistance of the composites	601:638	This physical cross-linking network reduces the water absorption and improves the water resistance of the composites.
31536333	4	43	theme	composites	730:739	arg1	properties					712:721	the thermal decomposition properties	686:721	the thermal decomposition properties of the composites	686:739	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	5	44	theme	composite	1035:1043	arg1	rate					1006:1009	shape recovery rate	991:1009	shape recovery rate of 10% RC-SP reinforced composite	991:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	5	45	theme	%	1016:1016	arg1	composite					1035:1043	10% RC-SP reinforced composite	1014:1043	10% RC-SP reinforced composite	1014:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	4	46	theme	decomposition	698:710	arg1	properties					712:721	the thermal decomposition properties	686:721	the thermal decomposition properties of the composites	686:739	The incorporation of RC-SP not only improves the thermal decomposition properties of the composites, but also enhances the mechanical properties and dynamic mechanical properties, attributed to the strong interaction between the filler and the matrix.
31536333	5	47	theme	shape	966:970	arg1	effect					979:984	good water-induced shape memory effect	947:984	good water-induced shape memory effect	947:984	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	5	48	theme	10	1014:1015	arg1	%					1016:1016	%	1016:1016	%	1016:1016	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	5	49	theme	recovery	997:1004	arg1	rate					1006:1009	shape recovery rate	991:1009	shape recovery rate of 10% RC-SP reinforced composite	991:1043	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	1	50	with	biocomposites	198:210	arg1	RC-SP					254:258	RC-SP	254:258	RC-SP	254:258	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	1	50	with	biocomposites	198:210	arg1	pulp					248:251	regenerated cellulose-softwood pulp	217:251	regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement	217:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	3	51	theme	physical	528:535	arg1	network					551:557	This physical cross-linking network	523:557	This physical cross-linking network	523:557	This physical cross-linking network reduces the water absorption and improves the water resistance of the composites.
31536333	6	52	theme	method	1168:1173	arg1	implementation					1133:1146	the implementation	1129:1146	the implementation of co-precipitation method and the application of renewable cellulose resources	1129:1226	This work provides useful information for the implementation of co-precipitation method and the application of renewable cellulose resources.
31536333	0	53	dep	Strong	13:18	arg1	Water-Induced					37:49	Water-Induced	37:49	Water-Induced	37:49	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	0	53	dep	Strong	13:18	arg1	Heat-Resistant					21:34	Heat-Resistant	21:34	Heat-Resistant	21:34	Mechanically Strong, Heat-Resistant, Water-Induced Shape Memory Poly(vinyl alcohol)/Regenerated Cellulose Biocomposites via a Facile Co-precipitation Method.
31536333	1	54	theme	vinyl	177:181	arg1	poly					172:175	poly	172:175	poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement	172:284	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	1	54	theme	vinyl	177:181	arg1	alcohol					183:189	vinyl alcohol	177:189	vinyl alcohol	177:189	In this work, poly(vinyl alcohol) (PVA) biocomposites with regenerated cellulose-softwood pulp (RC-SP) as a green reinforcement were prepared via co-precipitation method.
31536333	5	55	theme	PVA/RC-SP	918:926	arg1	composites					928:937	the fabricated PVA/RC-SP composites	903:937	the fabricated PVA/RC-SP composites	903:937	Moreover, the fabricated PVA/RC-SP composites exhibit good water-induced shape memory effect, and shape recovery rate of 10% RC-SP reinforced composite reaches 95.3% after immersing for 35 min.
31536333	3	56	theme	composites	629:638	arg1	resistance					611:620	the water resistance	601:620	the water resistance of the composites	601:638	This physical cross-linking network reduces the water absorption and improves the water resistance of the composites.
31536333	2	57	theme	molecular	441:449	arg1	entanglements					457:469	strong molecular chain entanglements	434:469	strong molecular chain entanglements	434:469	Simultaneous precipitation of the two components promotes uniform dispersion of the RC-SP and constructs strong molecular chain entanglements and hydrogen bonding network inside the composites.
31751742	8	0	theme	production	1212:1221	arg1	analysis					1223:1230	Short-chain fatty acids (SCFAs) production analysis	1180:1230	Short-chain fatty acids (SCFAs) production analysis	1180:1230	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	0	1	theme	gut	68:70	arg1	microbiota					72:81	gut microbiota	68:81	gut microbiota	68:81	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	4	2	theme	gut	662:664	arg1	microbiota					666:675	gut microbiota	662:675	gut microbiota	662:675	The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system.
31751742	9	3	from	function	1415:1422	arg1	microbiota					1431:1440	gut microbiota	1427:1440	gut microbiota	1427:1440	In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed.
31751742	5	4	theme	cecal	878:882	arg1	microbiota					884:893	mouse cecal microbiota	872:893	mouse cecal microbiota	872:893	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	7	5	theme	no	1125:1126	arg1	source					1135:1140	no carbon source	1125:1140	no carbon source	1125:1140	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	0	6	from	Effects	0:6	arg1	mouse					62:66	mouse	62:66	mouse	62:66	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	0	6	from	Effects	0:6	arg1	human					52:56	human	52:56	human	52:56	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	3	7	theme	protein	527:533	arg1	%					579:579	90.35 ± 1.35%	567:579	90.35 ± 1.35%	567:579	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	3	7	theme	protein	527:533	arg1	content					501:507	The content	497:507	The content of carbohydrates, protein, and uronic acids in EPS-RB	497:561	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	4	8	theme	batch	712:716	arg1	system					731:736	an in vitro batch fermentation system	700:736	an in vitro batch fermentation system	700:736	The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system.
31751742	3	9	theme	acids	547:551	arg1	%					579:579	90.35 ± 1.35%	567:579	90.35 ± 1.35%	567:579	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	3	9	theme	acids	547:551	arg1	content					501:507	The content	497:507	The content of carbohydrates, protein, and uronic acids in EPS-RB	497:561	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	7	10	dep	group	1115:1119	arg1	VI					1121:1122	VI	1121:1122	VI	1121:1122	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	7	10	dep	group	1115:1119	arg1	starch					1152:1157	starch	1152:1157	starch	1152:1157	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	3	11	from	content	501:507	arg1	EPS-RB					556:561	EPS-RB	556:561	EPS-RB	556:561	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	5	12	theme	mouse	872:876	arg1	microbiota					884:893	mouse cecal microbiota	872:893	mouse cecal microbiota	872:893	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	3	13	theme	carbohydrates	512:524	arg1	%					579:579	90.35 ± 1.35%	567:579	90.35 ± 1.35%	567:579	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	3	13	theme	carbohydrates	512:524	arg1	content					501:507	The content	497:507	The content of carbohydrates, protein, and uronic acids in EPS-RB	497:561	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	2	14	theme	molecular	444:452	arg1	ratio					454:458	their molecular ratio	438:458	their molecular ratio	438:458	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	2	15	theme	composition	248:258	arg1	analysis					260:267	Monosaccharide composition analysis	233:267	Monosaccharide composition analysis	233:267	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	2	16	theme	novel	295:299	arg1	EPS					301:303	a novel EPS	293:303	a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid	293:431	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	2	16	theme	novel	295:299	arg1	EPS-RB					283:288	EPS-RB	283:288	EPS-RB	283:288	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	2	17	theme	D-glucuronic	415:426	arg1	acid					428:431	D-glucuronic acid	415:431	D-glucuronic acid	415:431	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	4	18	theme	in	703:704	arg1	system					731:736	an in vitro batch fermentation system	700:736	an in vitro batch fermentation system	700:736	The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system.
31751742	2	19	theme	D-galactose/N-acetyl	346:365	arg1	glucosamine					367:377	D-galactose/N-acetyl glucosamine	346:377	D-galactose/N-acetyl glucosamine	346:377	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	0	20	theme	Escherichia	14:24	arg1	exopolysaccharide					31:47	an Escherichia coli exopolysaccharide	11:47	an Escherichia coli exopolysaccharide	11:47	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	5	21	theme	EPS-RB	771:776	arg1	EPS-RB					771:776	EPS-RB	771:776	EPS-RB	771:776	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	5	21	theme	EPS-RB	771:776	arg1	%					766:766	~96%	763:766	~96% of EPS-RB	763:776	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	8	22	theme	acids	1198:1202	arg1	production					1212:1221	Short-chain fatty acids (SCFAs) production	1180:1221	Short-chain fatty acids (SCFAs) production analysis	1180:1230	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	4	23	theme	fermentation	718:729	arg1	system					731:736	an in vitro batch fermentation system	700:736	an in vitro batch fermentation system	700:736	The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system.
31751742	6	24	theme	high-throughput	909:923	arg1	sequencing					925:934	high-throughput sequencing	909:934	high-throughput sequencing	909:934	Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota.
31751742	9	25	theme	gut	1427:1429	arg1	microbiota					1431:1440	gut microbiota	1427:1440	gut microbiota	1427:1440	In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed.
31751742	3	26	theme	uronic	540:545	arg1	acids					547:551	uronic acids	540:551	uronic acids	540:551	The content of carbohydrates, protein, and uronic acids in EPS-RB was 90.35 ± 1.35%, 2.62 ± 0.05% and 8.16 ± 1.00%, respectively.
31751742	9	27	theme	regulatory	1404:1413	arg1	function					1415:1422	its regulatory function	1400:1422	its regulatory function on gut microbiota	1400:1440	In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed.
31751742	0	28	theme	exopolysaccharide	31:47	arg1	Effects					0:6	Effects	0:6	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro	0:90	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	5	29	theme	human	797:801	arg1	microbiota					809:818	human fecal microbiota	797:818	human fecal microbiota	797:818	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	0	30	dep	human	52:56	arg1	microbiota					72:81	gut microbiota	68:81	gut microbiota	68:81	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	1	31	theme	gene	170:173	arg1	ycjD-fabI-yciW-rnb					183:200	the gene cluster ycjD-fabI-yciW-rnb	166:200	the gene cluster ycjD-fabI-yciW-rnb	166:200	In this study, an exopolysaccharide (EPS) named EPS-RB was produced when the gene cluster ycjD-fabI-yciW-rnb were overexpressed in E. coli.
31751742	8	32	theme	group	1292:1296	arg1	VIR					1298:1300	group VIR	1292:1300	group VIR	1292:1300	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	1	33	theme	cluster	175:181	arg1	ycjD-fabI-yciW-rnb					183:200	the gene cluster ycjD-fabI-yciW-rnb	166:200	the gene cluster ycjD-fabI-yciW-rnb	166:200	In this study, an exopolysaccharide (EPS) named EPS-RB was produced when the gene cluster ycjD-fabI-yciW-rnb were overexpressed in E. coli.
31751742	9	34	from	EPS-RB	1365:1370	arg1	coli					1378:1381	E. coli	1375:1381	E. coli	1375:1381	In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed.
31751742	8	35	theme	fatty	1192:1196	arg1	acids					1198:1202	Short-chain fatty acids	1180:1202	Short-chain fatty acids (SCFAs) production analysis	1180:1230	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	8	35	theme	fatty	1192:1196	arg1	SCFAs					1205:1209	SCFAs	1205:1209	SCFAs	1205:1209	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	7	36	theme	carbon	1081:1086	arg1	source					1088:1093	a carbon source	1079:1093	a carbon source	1079:1093	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	7	37	theme	group	1058:1062	arg1	EPS-RB					1069:1074	EPS-RB	1069:1074	EPS-RB	1069:1074	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	7	37	theme	group	1058:1062	arg1	VIR					1064:1066	group VIR	1058:1066	group VIR (EPS-RB as a carbon source)	1058:1094	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	7	38	theme	carbon	1128:1133	arg1	source					1135:1140	no carbon source	1125:1140	no carbon source	1125:1140	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	8	39	theme	Short-chain	1180:1190	arg1	acids					1198:1202	Short-chain fatty acids	1180:1202	Short-chain fatty acids (SCFAs) production analysis	1180:1230	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	8	39	theme	Short-chain	1180:1190	arg1	SCFAs					1205:1209	SCFAs	1205:1209	SCFAs	1205:1209	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	0	40	dep	Escherichia	14:24	arg1	coli					26:29	coli	26:29	coli	26:29	Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro.
31751742	2	41	theme	Monosaccharide	233:246	arg1	analysis					260:267	Monosaccharide composition analysis	233:267	Monosaccharide composition analysis	233:267	Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic acid, and their molecular ratio was approximately 80:3:53:69:1:2:64.
31751742	7	42	theme	carbon	1164:1169	arg1	source					1171:1176	a carbon source	1162:1176	a carbon source	1162:1176	The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source).
31751742	4	43	dep	in	703:704	arg1	vitro					706:710	vitro	706:710	vitro	706:710	The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system.
31751742	5	44	theme	fecal	803:807	arg1	microbiota					809:818	human fecal microbiota	797:818	human fecal microbiota	797:818	The results showed that ~96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota.
31751742	8	45	dep	groups	1307:1312	arg1	VIS					1321:1323	VIS	1321:1323	VIS	1321:1323	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	8	45	dep	groups	1307:1312	arg1	VI					1314:1315	VI	1314:1315	VI	1314:1315	Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation.
31751742	6	46	theme	gut	975:977	arg1	microbiota					979:988	the human gut microbiota	965:988	the human gut microbiota	965:988	Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota.
31751742	6	47	theme	human	969:973	arg1	microbiota					979:988	the human gut microbiota	965:988	the human gut microbiota	965:988	Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota.
31885873	4	0	from	growth	620:625	arg1	intestine					634:642	the intestine	630:642	the intestine	630:642	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	5	1	from	Indian	707:712	arg1	sequencing					661:670	Next generation sequencing	645:670	Next generation sequencing of fecal samples from Japanese and Indian adults	645:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	5	1	from	Indian	707:712	arg1	samples					681:687	fecal samples	675:687	fecal samples from Japanese and Indian adults	675:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	13	2	theme	growth	1269:1274	arg1	response					1276:1283	a growth response	1267:1283	a growth response to xylose, but not to arabinose	1267:1315	exhibited a growth response to xylose, but not to arabinose, whereas P. copri proliferated in response to both xylose and arabinose.
31885873	4	3	theme	components	585:594	arg1	utilization					562:572	utilization	562:572	utilization of dietary components driving their efficient growth in the intestine	562:642	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	16	4	theme	microbiota	1622:1631	arg1	members					1607:1613	fungal members	1600:1613	fungal members of gut microbiota	1600:1631	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	1	5	theme	many	165:168	arg1	aspects					170:176	many aspects	165:176	many aspects of biological processes such as nutrition and activation of adaptive immunity	165:254	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	4	6	theme	dietary	577:583	arg1	components					585:594	dietary components	577:594	dietary components driving their efficient growth in the intestine	577:642	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	16	7	theme	gut	1618:1620	arg1	microbiota					1622:1631	gut microbiota	1618:1631	gut microbiota	1618:1631	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	5	8	theme	fecal	675:679	arg1	samples					681:687	fecal samples	675:687	fecal samples from Japanese and Indian adults	675:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	6	9	theme	plant	830:834	arg1	polysaccharides					836:850	more plant polysaccharides	825:850	more plant polysaccharides	825:850	In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida.
31885873	10	10	dep	grown	1070:1074	arg1	promoted					1094:1101	promoted	1094:1101	promoted rapid proliferation of Prevotella copri	1094:1141	grown with arabinoxylan promoted rapid proliferation of Prevotella copri.
31885873	3	11	theme	bacterium	368:376	arg1	interaction					353:363	the interaction	349:363	the interaction of bacterium and fungus	349:387	Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown.
31885873	5	12	from	Japanese	694:701	arg1	sequencing					661:670	Next generation sequencing	645:670	Next generation sequencing of fecal samples from Japanese and Indian adults	645:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	5	12	from	Japanese	694:701	arg1	samples					681:687	fecal samples	675:687	fecal samples from Japanese and Indian adults	675:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	5	13	theme	samples	681:687	arg1	sequencing					661:670	Next generation sequencing	645:670	Next generation sequencing of fecal samples from Japanese and Indian adults	645:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	6	14	theme	more	825:828	arg1	polysaccharides					836:850	more plant polysaccharides	825:850	more plant polysaccharides	825:850	In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida.
31885873	11	15	from	factor	1200:1205	arg1	supernatants					1230:1241	the Candida culture supernatants	1210:1241	the Candida culture supernatants	1210:1241	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	5	16	dep	Japanese	694:701	arg1	adults					714:719	adults	714:719	adults	714:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	11	17	theme	culture	1222:1228	arg1	supernatants					1230:1241	the Candida culture supernatants	1210:1241	the Candida culture supernatants	1210:1241	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	13	18	dep	exhibited	1257:1265	arg1	whereas					1318:1324	whereas	1318:1324	whereas	1318:1324	exhibited a growth response to xylose, but not to arabinose, whereas P. copri proliferated in response to both xylose and arabinose.
31885873	5	19	theme	fungal	769:774	arg1	composition					776:786	bacterial and fungal composition	755:786	bacterial and fungal composition	755:786	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	6	20	theme	Candida	912:918	arg1	numbers					886:892	increased numbers	876:892	increased numbers of Prevotella and Candida	876:918	In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida.
31885873	2	21	theme	commensal	270:278	arg1	fungi					280:284	commensal fungi	270:284	commensal fungi residing in the intestine	270:310	In addition, commensal fungi residing in the intestine also influence host health.
31885873	5	22	from	sequencing	661:670	arg1	Indian					707:712	Indian	707:712	Indian	707:712	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	5	22	from	sequencing	661:670	arg1	Japanese					694:701	Japanese	694:701	Japanese	694:701	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	5	23	theme	Next	645:648	arg1	sequencing					661:670	Next generation sequencing	645:670	Next generation sequencing of fecal samples from Japanese and Indian adults	645:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	16	24	dep	proof	1578:1582	arg1	facilitate					1637:1646	facilitate	1637:1646	can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite	1633:1756	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	16	25	theme	intestine	1670:1678	arg1	colonization					1650:1661	a colonization	1648:1661	a colonization	1648:1661	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	1	26	theme	biological	181:190	arg1	activation					224:233	activation	224:233	activation	224:233	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	1	26	theme	biological	181:190	arg1	processes					192:200	biological processes	181:200	biological processes such as nutrition and activation of adaptive immunity	181:254	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	1	26	theme	biological	181:190	arg1	nutrition					210:218	nutrition	210:218	nutrition	210:218	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	14	27	theme	Candida	1390:1396	arg1	spp.					1398:1401	Candida spp.	1390:1401	Candida spp.	1390:1401	Candida spp., but not P. copri, colonized the intestine of germ-free mice.
31885873	5	28	theme	generation	650:659	arg1	sequencing					661:670	Next generation sequencing	645:670	Next generation sequencing of fecal samples from Japanese and Indian adults	645:719	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	16	29	theme	concept	1587:1593	arg1	proof					1578:1582	a proof	1576:1582	a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite	1576:1756	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	10	30	theme	rapid	1103:1107	arg1	proliferation					1109:1121	rapid proliferation	1103:1121	rapid proliferation of Prevotella copri	1103:1141	grown with arabinoxylan promoted rapid proliferation of Prevotella copri.
31885873	14	31	theme	mice	1459:1462	arg1	intestine					1436:1444	the intestine	1432:1444	the intestine of germ-free mice	1432:1462	Candida spp., but not P. copri, colonized the intestine of germ-free mice.
31885873	1	32	theme	processes	192:200	arg1	aspects					170:176	many aspects	165:176	many aspects of biological processes such as nutrition and activation of adaptive immunity	165:254	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	15	33	theme	P.	1474:1475	arg1	copri					1477:1481	P. copri	1474:1481	P. copri	1474:1481	However, P. copri successfully colonized mouse intestine already harboring Candida.
31885873	14	34	theme	germ-free	1449:1457	arg1	mice					1459:1462	germ-free mice	1449:1462	germ-free mice	1449:1462	Candida spp., but not P. copri, colonized the intestine of germ-free mice.
31885873	3	35	theme	intestine	460:468	arg1	colonization					434:445	colonization	434:445	colonization of the human intestine	434:468	Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown.
31885873	3	36	theme	fungus	382:387	arg1	interaction					353:363	the interaction	349:363	the interaction of bacterium and fungus	349:387	Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown.
31885873	6	37	theme	Prevotella	897:906	arg1	numbers					886:892	increased numbers	876:892	increased numbers of Prevotella and Candida	876:918	In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida.
31885873	8	38	theme	strong	941:946	arg1	responses					955:963	strong growth responses	941:963	strong growth responses	941:963	showed strong growth responses to the plant polysaccharide arabinoxylan in vitro.
31885873	0	39	theme	intestinal	34:43	arg1	microbiota					45:54	Indian intestinal microbiota	27:54	Indian intestinal microbiota	27:54	Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
31885873	5	40	theme	differential	730:741	arg1	patterns					743:750	differential patterns	730:750	differential patterns of bacterial and fungal composition	730:786	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	11	41	theme	potential	1174:1182	arg1	Arabinose					1144:1152	Arabinose	1144:1152	Arabinose	1144:1152	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	11	41	theme	potential	1174:1182	arg1	factor					1200:1205	a potential growth-inducing factor	1172:1205	a potential growth-inducing factor in the Candida culture supernatants	1172:1241	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	0	42	theme	Indian	27:32	arg1	microbiota					45:54	Indian intestinal microbiota	27:54	Indian intestinal microbiota	27:54	Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
31885873	4	43	theme	bacterial	529:537	arg1	species					550:556	bacterial and fungal species	529:556	bacterial and fungal species	529:556	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	3	44	theme	precise	409:415	arg1	mechanism					417:425	its precise mechanism	405:425	its precise mechanism during colonization of the human intestine	405:468	Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown.
31885873	5	45	theme	bacterial	755:763	arg1	composition					776:786	bacterial and fungal composition	755:786	bacterial and fungal composition	755:786	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	11	46	theme	growth-inducing	1184:1198	arg1	Arabinose					1144:1152	Arabinose	1144:1152	Arabinose	1144:1152	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	11	46	theme	growth-inducing	1184:1198	arg1	factor					1200:1205	a potential growth-inducing factor	1172:1205	a potential growth-inducing factor in the Candida culture supernatants	1172:1241	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	16	47	theme	fungal	1600:1605	arg1	members					1607:1613	fungal members	1600:1613	fungal members of gut microbiota	1600:1631	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	3	48	theme	human	454:458	arg1	intestine					460:468	the human intestine	450:468	the human intestine	450:468	Although the interaction of bacterium and fungus has been shown, its precise mechanism during colonization of the human intestine remains largely unknown.
31885873	16	49	theme	bacterial	1689:1697	arg1	counterparts					1699:1710	their bacterial counterparts	1683:1710	their bacterial counterparts	1683:1710	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	6	50	theme	increased	876:884	arg1	numbers					886:892	increased numbers	876:892	increased numbers of Prevotella and Candida	876:918	In particular, Indians, who consume more plant polysaccharides than Japanese, harbored increased numbers of Prevotella and Candida.
31885873	8	51	theme	growth	948:953	arg1	responses					955:963	strong growth responses	941:963	strong growth responses	941:963	showed strong growth responses to the plant polysaccharide arabinoxylan in vitro.
31885873	1	52	theme	bacterial	121:129	arg1	species					131:137	The bacterial species	117:137	The bacterial species living in the gut	117:155	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	0	53	theme	microbiota	45:54	arg1	Comparison					0:9	Comparison	0:9	Comparison of Japanese and Indian intestinal microbiota	0:54	Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
31885873	4	54	theme	fungal	543:548	arg1	species					550:556	bacterial and fungal species	529:556	bacterial and fungal species	529:556	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	9	55	theme	culture	1033:1039	arg1	supernatants					1041:1052	the culture supernatants	1029:1052	the culture supernatants of Candida	1029:1063	Furthermore, the culture supernatants of Candida spp.
31885873	9	56	theme	Candida	1057:1063	arg1	supernatants					1041:1052	the culture supernatants	1029:1052	the culture supernatants of Candida	1029:1063	Furthermore, the culture supernatants of Candida spp.
31885873	11	57	theme	Candida	1214:1220	arg1	supernatants					1230:1241	the Candida culture supernatants	1210:1241	the Candida culture supernatants	1210:1241	Arabinose was identified as a potential growth-inducing factor in the Candida culture supernatants.
31885873	8	58	theme	polysaccharide	978:991	arg1	arabinoxylan					993:1004	the plant polysaccharide arabinoxylan	968:1004	the plant polysaccharide arabinoxylan	968:1004	showed strong growth responses to the plant polysaccharide arabinoxylan in vitro.
31885873	2	59	theme	host	327:330	arg1	health					332:337	host health	327:337	host health	327:337	In addition, commensal fungi residing in the intestine also influence host health.
31885873	13	60	dep	xylose	1368:1373	arg1	response					1351:1358	response	1351:1358	response	1351:1358	exhibited a growth response to xylose, but not to arabinose, whereas P. copri proliferated in response to both xylose and arabinose.
31885873	4	61	theme	efficient	610:618	arg1	growth					620:625	their efficient growth	604:625	their efficient growth in the intestine	604:642	Here, we show interaction between bacterial and fungal species for utilization of dietary components driving their efficient growth in the intestine.
31885873	5	62	theme	composition	776:786	arg1	patterns					743:750	differential patterns	730:750	differential patterns of bacterial and fungal composition	730:786	Next generation sequencing of fecal samples from Japanese and Indian adults revealed differential patterns of bacterial and fungal composition.
31885873	16	63	theme	dietary	1739:1745	arg1	metabolite					1747:1756	a dietary metabolite	1737:1756	a dietary metabolite	1737:1756	These findings demonstrate a proof of concept that fungal members of gut microbiota can facilitate a colonization of the intestine by their bacterial counterparts, potentially mediated by a dietary metabolite.
31885873	15	64	theme	mouse	1506:1510	arg1	intestine					1512:1520	mouse intestine	1506:1520	mouse intestine already harboring Candida	1506:1546	However, P. copri successfully colonized mouse intestine already harboring Candida.
31885873	8	65	theme	plant	972:976	arg1	arabinoxylan					993:1004	the plant polysaccharide arabinoxylan	968:1004	the plant polysaccharide arabinoxylan	968:1004	showed strong growth responses to the plant polysaccharide arabinoxylan in vitro.
31885873	1	66	theme	adaptive	238:245	arg1	immunity					247:254	adaptive immunity	238:254	adaptive immunity	238:254	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	0	67	theme	diet-dependent	62:75	arg1	interaction					77:87	diet-dependent interaction	62:87	diet-dependent interaction between bacteria and fungi	62:114	Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
31885873	10	68	theme	copri	1137:1141	arg1	proliferation					1109:1121	rapid proliferation	1103:1121	rapid proliferation of Prevotella copri	1103:1141	grown with arabinoxylan promoted rapid proliferation of Prevotella copri.
31885873	0	69	theme	Japanese	14:21	arg1	Comparison					0:9	Comparison	0:9	Comparison of Japanese and Indian intestinal microbiota	0:54	Comparison of Japanese and Indian intestinal microbiota shows diet-dependent interaction between bacteria and fungi.
31885873	1	70	theme	immunity	247:254	arg1	activation					224:233	activation	224:233	activation	224:233	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31885873	1	70	theme	immunity	247:254	arg1	nutrition					210:218	nutrition	210:218	nutrition	210:218	The bacterial species living in the gut mediate many aspects of biological processes such as nutrition and activation of adaptive immunity.
31161381	10	0	theme	lucidum	1254:1260	arg1	mycelium					1262:1269	G. lucidum mycelium	1251:1269	G. lucidum mycelium obtained from this study	1251:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	8	1	theme	weight-average	1074:1087	arg1	weight					1099:1104	The weight-average molecular weight	1070:1104	The weight-average molecular weight of component 2 in the extracellular polysaccharide	1070:1155	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	8	1	theme	weight-average	1074:1087	arg1	5.554 × 104 Da					1161:1174	5.554 × 104 Da	1161:1174	5.554 × 104 Da	1161:1174	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	11	2	theme	good	1423:1426	arg1	activity					1442:1449	good immunological activity	1423:1449	good immunological activity	1423:1449	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	4	3	theme	intracellular	572:584	arg1	yield					601:605	the average intracellular polysaccharide yield	560:605	the average intracellular polysaccharide yield	560:605	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	4	3	theme	intracellular	572:584	arg1	similar					622:628	similar	622:628	similar	622:628	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	7	4	theme	intracellular	985:997	arg1	polysaccharide					1005:1018	the intracellular crude polysaccharide	981:1018	the intracellular crude polysaccharide	981:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	2	5	contain	contains	285:292	arg2	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	2	5	contain	contains	285:292	arg1	medium					243:248	The accordingly optimized fermentation medium	204:248	The accordingly optimized fermentation medium by central composite design method	204:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	4	6	theme	average	564:570	arg1	yield					601:605	the average intracellular polysaccharide yield	560:605	the average intracellular polysaccharide yield	560:605	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	4	6	theme	average	564:570	arg1	similar					622:628	similar	622:628	similar	622:628	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	5	7	theme	intracellular	668:680	arg1	polysaccharides					682:696	intracellular polysaccharides	668:696	intracellular polysaccharides in the 5-L and 50-L fermentors	668:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	2	8	theme	12 g L-1	325:332	arg1	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	7	9	theme	crude	999:1003	arg1	polysaccharide					1005:1018	the intracellular crude polysaccharide	981:1018	the intracellular crude polysaccharide	981:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	10	10	theme	intracellular	1335:1347	arg1	polysaccharides					1349:1363	intracellular polysaccharides	1335:1363	intracellular polysaccharides	1335:1363	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	12	11	theme	optimized	1469:1477	arg1	process					1479:1485	the optimized process	1465:1485	the optimized process	1465:1485	Conceivably, the optimized process can be applied for the large-scale production.
31161381	4	12	theme	flask	527:531	arg1	experiments					533:543	Shake flask experiments	521:543	Shake flask experiments	521:543	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	5	13	theme	polysaccharides	682:696	arg1	yields					658:663	The yields	654:663	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors	654:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	5	13	theme	polysaccharides	682:696	arg1	2.59 g L-1					734:743	2.59 g L-1	734:743	2.59 g L-1	734:743	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	2	14	theme	powder	318:323	arg1	12 g L-1					325:332	yeast powder 12 g L-1	312:332	yeast powder 12 g L-1	312:332	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	8	15	from	weight	1099:1104	arg1	polysaccharide					1142:1155	the extracellular polysaccharide	1124:1155	the extracellular polysaccharide	1124:1155	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	4	16	theme	Shake	521:525	arg1	experiments					533:543	Shake flask experiments	521:543	Shake flask experiments	521:543	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	12	17	theme	large-scale	1510:1520	arg1	production					1522:1531	the large-scale production	1506:1531	the large-scale production	1506:1531	Conceivably, the optimized process can be applied for the large-scale production.
31161381	8	18	theme	component	1109:1117	arg1	weight					1099:1104	The weight-average molecular weight	1070:1104	The weight-average molecular weight of component 2 in the extracellular polysaccharide	1070:1155	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	8	18	theme	component	1109:1117	arg1	5.554 × 104 Da					1161:1174	5.554 × 104 Da	1161:1174	5.554 × 104 Da	1161:1174	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	2	19	theme	yeast	312:316	arg1	12 g L-1					325:332	yeast powder 12 g L-1	312:332	yeast powder 12 g L-1	312:332	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	6	20	theme	weight	789:794	arg1	distribution					796:807	The molecular weight distribution	775:807	The molecular weight distribution of intracellular and extracellular polysaccharides obtained	775:867	The molecular weight distribution of intracellular and extracellular polysaccharides obtained was determined by HPSEC-MALLS-RI.
31161381	1	21	theme	Ganoderma	185:193	arg1	lucidum					195:201	Ganoderma lucidum	185:201	Ganoderma lucidum	185:201	The objective of this study was to increase the intracellular polysaccharide yield of Ganoderma lucidum.
31161381	10	22	theme	fermentation	1227:1238	arg1	process					1240:1246	The liquid submerged fermentation process	1206:1246	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study	1206:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	5	23	theme	5-L	705:707	arg1	fermentors					718:727	the 5-L and 50-L fermentors	701:727	the 5-L and 50-L fermentors	701:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	6	24	theme	molecular	779:787	arg1	distribution					796:807	The molecular weight distribution	775:807	The molecular weight distribution of intracellular and extracellular polysaccharides obtained	775:867	The molecular weight distribution of intracellular and extracellular polysaccharides obtained was determined by HPSEC-MALLS-RI.
31161381	1	25	theme	lucidum	195:201	arg1	yield					176:180	the intracellular polysaccharide yield	143:180	the intracellular polysaccharide yield of Ganoderma lucidum	143:201	The objective of this study was to increase the intracellular polysaccharide yield of Ganoderma lucidum.
31161381	10	26	theme	G.	1251:1252	arg1	mycelium					1262:1269	G. lucidum mycelium	1251:1269	G. lucidum mycelium obtained from this study	1251:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	2	27	theme	phosphate	356:364	arg1	3 g L-1					366:372	potassium dihydrogen phosphate 3 g L-1	335:372	potassium dihydrogen phosphate 3 g L-1	335:372	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	5	28	theme	50-L	713:716	arg1	fermentors					718:727	the 5-L and 50-L fermentors	701:727	the 5-L and 50-L fermentors	701:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	2	29	theme	dihydrogen	345:354	arg1	phosphate					356:364	potassium dihydrogen phosphate	335:364	potassium dihydrogen phosphate 3 g L-1	335:372	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	0	30	theme	lucidum	26:32	arg1	Optimization					0:11	Optimization	0:11	Optimization of Ganoderma lucidum	0:32	Optimization of Ganoderma lucidum Polysaccharides Fermentation Process for Large-Scale Production.
31161381	10	31	theme	mycelium	1262:1269	arg1	process					1240:1246	The liquid submerged fermentation process	1206:1246	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study	1206:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	8	32	theme	extracellular	1128:1140	arg1	polysaccharide					1142:1155	the extracellular polysaccharide	1124:1155	the extracellular polysaccharide	1124:1155	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	2	33	theme	potassium	335:343	arg1	phosphate					356:364	potassium dihydrogen phosphate	335:364	potassium dihydrogen phosphate 3 g L-1	335:372	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	0	34	theme	Ganoderma	16:24	arg1	lucidum					26:32	Ganoderma lucidum	16:32	Ganoderma lucidum	16:32	Optimization of Ganoderma lucidum Polysaccharides Fermentation Process for Large-Scale Production.
31161381	4	35	theme	predicted	637:645	arg1	value					647:651	the predicted value	633:651	the predicted value	633:651	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	11	36	theme	extracellular	1388:1400	arg1	polysaccharides					1402:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	1	37	theme	study	121:125	arg1	objective					103:111	The objective	99:111	The objective of this study	99:125	The objective of this study was to increase the intracellular polysaccharide yield of Ganoderma lucidum.
31161381	7	38	theme	weight-average	931:944	arg1	weight					956:961	the weight-average molecular weight	927:961	the weight-average molecular weight of component 1 in the intracellular crude polysaccharide	927:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	7	38	theme	weight-average	931:944	arg1	4.695 × 106 Da					1024:1037	4.695 × 106 Da	1024:1037	4.695 × 106 Da	1024:1037	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	1	39	theme	intracellular	147:159	arg1	yield					176:180	the intracellular polysaccharide yield	143:180	the intracellular polysaccharide yield of Ganoderma lucidum	143:201	The objective of this study was to increase the intracellular polysaccharide yield of Ganoderma lucidum.
31161381	0	40	theme	Fermentation	50:61	arg1	Process					63:69	Fermentation Process	50:69	Fermentation Process	50:69	Optimization of Ganoderma lucidum Polysaccharides Fermentation Process for Large-Scale Production.
31161381	5	41	from	yields	658:663	arg1	fermentors					718:727	the 5-L and 50-L fermentors	701:727	the 5-L and 50-L fermentors	701:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	2	42	theme	design	271:276	arg1	method					278:283	central composite design method	253:283	central composite design method	253:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	7	43	theme	molecular	946:954	arg1	weight					956:961	the weight-average molecular weight	927:961	the weight-average molecular weight of component 1 in the intracellular crude polysaccharide	927:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	7	43	theme	molecular	946:954	arg1	4.695 × 106 Da					1024:1037	4.695 × 106 Da	1024:1037	4.695 × 106 Da	1024:1037	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	3	44	theme	yield	499:503	arg1	value					461:465	the predicted value	447:465	the predicted value of intracellular polysaccharide yield	447:503	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	3	44	theme	yield	499:503	arg1	2.03 g L-1					509:518	2.03 g L-1	509:518	2.03 g L-1	509:518	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	3	45	theme	predicted	451:459	arg1	value					461:465	the predicted value	447:465	the predicted value of intracellular polysaccharide yield	447:503	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	3	45	theme	predicted	451:459	arg1	2.03 g L-1					509:518	2.03 g L-1	509:518	2.03 g L-1	509:518	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	10	46	theme	polysaccharides	1349:1363	arg1	yield					1326:1330	the yield	1322:1330	the yield of intracellular polysaccharides	1322:1363	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	2	47	theme	composite	261:269	arg1	method					278:283	central composite design method	253:283	central composite design method	253:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	8	48	theme	molecular	1089:1097	arg1	weight					1099:1104	The weight-average molecular weight	1070:1104	The weight-average molecular weight of component 2 in the extracellular polysaccharide	1070:1155	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	8	48	theme	molecular	1089:1097	arg1	5.554 × 104 Da					1161:1174	5.554 × 104 Da	1161:1174	5.554 × 104 Da	1161:1174	The weight-average molecular weight of component 2 in the extracellular polysaccharide was 5.554 × 104 Da.
31161381	4	49	theme	polysaccharide	586:599	arg1	yield					601:605	the average intracellular polysaccharide yield	560:605	the average intracellular polysaccharide yield	560:605	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	4	49	theme	polysaccharide	586:599	arg1	similar					622:628	similar	622:628	similar	622:628	Shake flask experiments confirmed that the average intracellular polysaccharide yield was 1.98 g L-1 similar to the predicted value.
31161381	10	50	theme	submerged	1217:1225	arg1	process					1240:1246	The liquid submerged fermentation process	1206:1246	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study	1206:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	3	51	theme	intracellular	470:482	arg1	yield					499:503	intracellular polysaccharide yield	470:503	intracellular polysaccharide yield	470:503	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	2	52	theme	central	253:259	arg1	method					278:283	central composite design method	253:283	central composite design method	253:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	2	53	theme	pH 5.5	383:388	arg1	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	11	54	theme	intracellular	1370:1382	arg1	polysaccharides					1402:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	5	55	from	fermentors	718:727	arg1	yields					658:663	The yields	654:663	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors	654:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	5	55	from	fermentors	718:727	arg1	2.59 g L-1					734:743	2.59 g L-1	734:743	2.59 g L-1	734:743	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	11	56	theme	immunological	1428:1440	arg1	activity					1442:1449	good immunological activity	1423:1449	good immunological activity	1423:1449	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	1	57	theme	polysaccharide	161:174	arg1	yield					176:180	the intracellular polysaccharide yield	143:180	the intracellular polysaccharide yield of Ganoderma lucidum	143:201	The objective of this study was to increase the intracellular polysaccharide yield of Ganoderma lucidum.
31161381	2	58	theme	initial	375:381	arg1	pH 5.5					383:388	initial pH 5.5	375:388	initial pH 5.5	375:388	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	2	59	theme	fermentation	230:241	arg1	medium					243:248	The accordingly optimized fermentation medium	204:248	The accordingly optimized fermentation medium by central composite design method	204:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	2	60	theme	40 g L-1	302:309	arg1	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	6	61	theme	polysaccharides	844:858	arg1	distribution					796:807	The molecular weight distribution	775:807	The molecular weight distribution of intracellular and extracellular polysaccharides obtained	775:867	The molecular weight distribution of intracellular and extracellular polysaccharides obtained was determined by HPSEC-MALLS-RI.
31161381	7	62	theme	mass	1047:1050	arg1	fraction					1052:1059	the mass fraction	1043:1059	the mass fraction	1043:1059	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	7	62	theme	mass	1047:1050	arg1	%					1067:1067	58%	1065:1067	58%	1065:1067	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	7	63	from	weight	956:961	arg1	polysaccharide					1005:1018	the intracellular crude polysaccharide	981:1018	the intracellular crude polysaccharide	981:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	2	64	theme	optimized	220:228	arg1	medium					243:248	The accordingly optimized fermentation medium	204:248	The accordingly optimized fermentation medium by central composite design method	204:283	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	2	65	theme	glucose	294:300	arg1	40 g L-1					302:309	glucose 40 g L-1	294:309	glucose 40 g L-1	294:309	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	6	66	theme	extracellular	830:842	arg1	polysaccharides					844:858	intracellular and extracellular polysaccharides	812:858	intracellular and extracellular polysaccharides obtained	812:867	The molecular weight distribution of intracellular and extracellular polysaccharides obtained was determined by HPSEC-MALLS-RI.
31161381	2	67	theme	3 g L-1	366:372	arg1	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	11	68	contain	have	1418:1421	arg2	activity					1442:1449	good immunological activity	1423:1449	good immunological activity	1423:1449	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	11	68	contain	have	1418:1421	arg1	polysaccharides					1402:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides	1366:1416	Its intracellular and extracellular polysaccharides have good immunological activity.
31161381	7	69	theme	component	966:974	arg1	weight					956:961	the weight-average molecular weight	927:961	the weight-average molecular weight of component 1 in the intracellular crude polysaccharide	927:1018	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	7	69	theme	component	966:974	arg1	4.695 × 106 Da					1024:1037	4.695 × 106 Da	1024:1037	4.695 × 106 Da	1024:1037	The results showed that the weight-average molecular weight of component 1 in the intracellular crude polysaccharide was 4.695 × 106 Da and the mass fraction was 58%.
31161381	5	70	from	polysaccharides	682:696	arg1	fermentors					718:727	the 5-L and 50-L fermentors	701:727	the 5-L and 50-L fermentors	701:727	The yields of intracellular polysaccharides in the 5-L and 50-L fermentors were 2.59 g L-1 and 2.65 g L-1, respectively.
31161381	2	71	theme	size	404:407	arg1	10 mL 100 mL-1					409:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1	294:422	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	3	72	theme	polysaccharide	484:497	arg1	yield					499:503	intracellular polysaccharide yield	470:503	intracellular polysaccharide yield	470:503	Under this condition, the predicted value of intracellular polysaccharide yield was 2.03 g L-1.
31161381	0	73	theme	Large-Scale	75:85	arg1	Production					87:96	Large-Scale Production	75:96	Large-Scale Production	75:96	Optimization of Ganoderma lucidum Polysaccharides Fermentation Process for Large-Scale Production.
31161381	10	74	theme	liquid	1210:1215	arg1	process					1240:1246	The liquid submerged fermentation process	1206:1246	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study	1206:1294	The liquid submerged fermentation process of G. lucidum mycelium obtained from this study has effectively increased the yield of intracellular polysaccharides.
31161381	6	75	theme	intracellular	812:824	arg1	polysaccharides					844:858	intracellular and extracellular polysaccharides	812:858	intracellular and extracellular polysaccharides obtained	812:867	The molecular weight distribution of intracellular and extracellular polysaccharides obtained was determined by HPSEC-MALLS-RI.
31161381	2	76	theme	inoculum	395:402	arg1	size					404:407	inoculum size	395:407	inoculum size	395:407	The accordingly optimized fermentation medium by central composite design method contains glucose 40 g L-1, yeast powder 12 g L-1, potassium dihydrogen phosphate 3 g L-1, initial pH 5.5, and inoculum size 10 mL 100 mL-1.
31161381	9	77	theme	mass	1181:1184	arg1	fraction					1186:1193	The mass fraction	1177:1193	The mass fraction	1177:1193	The mass fraction was 94.9%.
31161381	9	77	theme	mass	1181:1184	arg1	%					1203:1203	94.9%	1199:1203	94.9%	1199:1203	The mass fraction was 94.9%.
30986530	2	0	theme	Ca	625:626	arg1	ions					635:638	Si, Ca and Cu ions	621:638	ions	635:638	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	9	1	theme	safe	1969:1972	arg1	doses					1988:1992	safe but effective doses	1969:1992	safe but effective doses	1969:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	8	2	theme	glass	1763:1767	arg1	nanoparticles					1769:1781	copper-containing bioactive glass nanoparticles	1735:1781	copper-containing bioactive glass nanoparticles	1735:1781	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	9	3	from	able	1927:1930	arg1	manner					2010:2015	a controlled manner	1997:2015	a controlled manner	1997:2015	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	2	4	theme	Si	621:622	arg1	ions					635:638	Si, Ca and Cu ions	621:638	ions	635:638	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	9	5	theme	effective	1978:1986	arg1	doses					1988:1992	safe but effective doses	1969:1992	safe but effective doses	1969:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	4	6	theme	mineralized	1129:1139	arg1	deposition					1150:1159	mineralized collagen deposition	1129:1159	mineralized collagen deposition	1129:1159	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	8	7	theme	fibroin	1801:1807	arg1	composite					1809:1817	chitosan/silk fibroin composite	1787:1817	chitosan/silk fibroin composite	1787:1817	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	2	8	theme	Cu	632:633	arg1	ions					635:638	Si, Ca and Cu ions	621:638	ions	635:638	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	4	9	theme	deposition	1150:1159	arg1	formation					1087:1095	formation	1087:1095	formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors	1087:1251	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	6	10	theme	bone	1594:1597	arg1	repair					1599:1604	bone repair	1594:1604	bone repair	1594:1604	STATEMENT OF SIGNIFICANCE: Hydrogels loaded with cells and/or growth factors exhibit potential in bone repair.
30986530	1	11	theme	designed	215:222	arg1	compositions					224:235	designed compositions	215:235	designed compositions	215:235	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	12	theme	vascularized	1100:1111	arg1	tissue					1118:1123	vascularized bone tissue	1100:1123	vascularized bone tissue	1100:1123	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	1	13	theme	bone	397:400	arg1	repair					402:407	cell-free bone repair	387:407	cell-free bone repair	387:407	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	5	14	contain	have	1338:1341	arg2	ability					1375:1381	translation ability	1363:1381	translation ability	1363:1381	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	5	14	contain	have	1338:1341	arg2	potential					1349:1357	great potential	1343:1357	great potential	1343:1357	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	5	14	contain	have	1338:1341	arg1	hydrogels					1328:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	4	15	theme	tissue	1118:1123	arg1	formation					1087:1095	formation	1087:1095	formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors	1087:1251	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	3	16	theme	seeded	773:778	arg1	MC3T3-E1					780:787	seeded MC3T3-E1 and human umbilical vein endothelial cells	773:830	MC3T3-E1	780:787	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	9	17	from	manner	2010:2015	arg1	able					1927:1930	able	1927:1930	able	1927:1930	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	4	18	theme	8 weeks	1190:1196	arg1	period					1180:1185	a treatment period	1168:1185	a treatment period of 8 weeks without utilization of any cells and/or growth factors	1168:1251	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	9	19	dep	in	1890:1891	arg1	situ					1893:1896	situ	1893:1896	situ	1893:1896	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	2	20	theme	controlled	690:699	arg1	manner					701:706	a sustained and controlled manner	674:706	a sustained and controlled manner	674:706	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	2	21	from	doses	665:669	arg1	ions					635:638	Si, Ca and Cu ions	621:638	ions	635:638	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	5	22	theme	Cu-BG/CH/SF/GP	1303:1316	arg1	hydrogels					1328:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	3	23	theme	umbilical	799:807	arg1	cells					826:830	seeded MC3T3-E1 and human umbilical vein endothelial cells	773:830	cells	826:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	10	24	theme	growth	2256:2261	arg1	factors					2263:2269	expensive growth factors	2246:2269	expensive growth factors	2246:2269	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	1	25	theme	bioactive	168:176	arg1	nanoparticles					184:196	Copper-containing bioactive glass nanoparticles	150:196	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes	150:245	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	25	theme	bioactive	168:176	arg1	NPs					205:207	Cu-BG NPs	199:207	Cu-BG NPs	199:207	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	26	theme	defect	978:983	arg1	model					985:989	a critical-size rat calvarial bone defect model	943:989	a critical-size rat calvarial bone defect model	943:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	9	27	from	doses	1988:1992	arg1	ions					1961:1964	ions	1961:1964	ions at safe but effective doses	1961:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	9	27	from	doses	1988:1992	arg1	release					1950:1956	the release	1946:1956	the release of ions at safe but effective doses	1946:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	10	28	theme	novel	2293:2297	arg1	system					2308:2313	this novel hydrogel system	2288:2313	this novel hydrogel system	2288:2313	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	4	29	dep	In	914:915	arg1	vivo					917:920	vivo	917:920	vivo	917:920	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	2	30	dep	respective	649:658	arg1	safe					660:663	safe	660:663	safe	660:663	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	7	31	theme	related	1648:1654	arg1	obstacles					1638:1646	obstacles	1638:1646	obstacles related to the clinic translation	1638:1680	However, they have been facing obstacles related to the clinic translation.
30986530	0	32	theme	bone	104:107	arg1	formation					109:117	in-situ bone formation	96:117	in-situ bone formation in rat calvarial bone defects	96:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	1	33	theme	Cu-BG	199:203	arg1	nanoparticles					184:196	Copper-containing bioactive glass nanoparticles	150:196	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes	150:245	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	33	theme	Cu-BG	199:203	arg1	NPs					205:207	Cu-BG NPs	199:207	Cu-BG NPs	199:207	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	34	theme	critical-size	945:957	arg1	model					985:989	a critical-size rat calvarial bone defect model	943:989	a critical-size rat calvarial bone defect model	943:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	10	35	theme	great	2319:2323	arg1	potential					2325:2333	great potential	2319:2333	great potential for translation to the clinic	2319:2363	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	3	36	theme	endothelial	814:824	arg1	cells					826:830	seeded MC3T3-E1 and human umbilical vein endothelial cells	773:830	cells	826:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	10	37	theme	hydrogel	2299:2306	arg1	system					2308:2313	this novel hydrogel system	2288:2313	this novel hydrogel system	2288:2313	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	1	38	theme	injectable	362:371	arg1	hydrogels					373:381	injectable hydrogels	362:381	injectable hydrogels	362:381	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	39	with	nanoparticles	184:196	arg1	sizes					241:245	sizes	241:245	sizes	241:245	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	39	with	nanoparticles	184:196	arg1	compositions					224:235	designed compositions	215:235	designed compositions	215:235	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	40	theme	calvarial	963:971	arg1	model					985:989	a critical-size rat calvarial bone defect model	943:989	a critical-size rat calvarial bone defect model	943:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	0	41	theme	calvarial	126:134	arg1	defects					141:147	rat calvarial bone defects	122:147	rat calvarial bone defects	122:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	10	42	contain	has	2315:2317	arg1	system					2308:2313	this novel hydrogel system	2288:2313	this novel hydrogel system	2288:2313	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	10	42	contain	has	2315:2317	arg2	potential					2325:2333	great potential	2319:2333	great potential for translation to the clinic	2319:2363	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	2	43	theme	well-defined	466:477	arg1	injectability					479:491	well-defined injectability	466:491	well-defined injectability	466:491	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	3	44	dep	In	709:710	arg1	vitro					712:716	vitro	712:716	vitro	712:716	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	2	45	theme	sustained	676:684	arg1	manner					701:706	a sustained and controlled manner	674:706	a sustained and controlled manner	674:706	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	5	46	theme	bone	1387:1390	arg1	regeneration					1392:1403	bone regeneration	1387:1403	bone regeneration owing to their thermo-sensitive properties	1387:1446	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	1	47	theme	/silk	299:303	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	5	48	theme	great	1343:1347	arg1	potential					1349:1357	great potential	1343:1357	great potential	1343:1357	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	0	49	theme	injectable	20:29	arg1	chitosan/silk					31:43	Thermally triggered injectable chitosan/silk	0:43	Thermally triggered injectable chitosan/silk	0:43	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	2	50	theme	Cu-BG/CH/SF/GP	424:437	arg1	gels					439:442	The resulting Cu-BG/CH/SF/GP gels	410:442	The resulting Cu-BG/CH/SF/GP gels	410:442	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	4	51	theme	Cu-BG/CH/SF/GP	1021:1034	arg1	able					1044:1047	able	1044:1047	able	1044:1047	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	4	51	theme	Cu-BG/CH/SF/GP	1021:1034	arg1	gel					1036:1038	the optimal Cu-BG/CH/SF/GP gel	1009:1038	the optimal Cu-BG/CH/SF/GP gel	1009:1038	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	0	52	theme	nanoparticle	69:80	arg1	hydrogels					82:90	glass nanoparticle hydrogels	63:90	glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects	63:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	10	53	theme	bone	2166:2169	arg1	defects					2171:2177	critical-size rat calvarial bone defects	2138:2177	critical-size rat calvarial bone defects	2138:2177	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	5	54	theme	cell-free	1449:1457	arg1	bioactivity					1459:1469	cell-free bioactivity	1449:1469	cell-free bioactivity	1449:1469	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	6	55	theme	growth	1558:1563	arg1	factors					1565:1571	growth factors	1558:1571	growth factors	1558:1571	STATEMENT OF SIGNIFICANCE: Hydrogels loaded with cells and/or growth factors exhibit potential in bone repair.
30986530	9	56	theme	in	1890:1891	arg1	properties					1907:1916	injectability and thermally triggered in situ gelation properties	1852:1916	injectability and thermally triggered in situ gelation properties	1852:1916	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	10	57	theme	rat	2152:2154	arg1	defects					2171:2177	critical-size rat calvarial bone defects	2138:2177	critical-size rat calvarial bone defects	2138:2177	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	8	58	theme	system	1714:1719	arg1	type					1697:1700	a novel type	1689:1700	a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite	1689:1817	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	9	59	theme	controlled	1999:2008	arg1	manner					2010:2015	a controlled manner	1997:2015	a controlled manner	1997:2015	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	10	60	theme	optimal	2091:2097	arg1	gel					2099:2101	The optimal gel	2087:2101	The optimal gel	2087:2101	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	3	61	theme	In	709:710	arg1	studies					718:724	In vitro studies	709:724	In vitro studies	709:724	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	1	62	theme	chitosan	286:293	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	63	theme	bone	1070:1073	arg1	defect					1075:1080	the bone defect	1066:1080	the bone defect	1066:1080	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	8	64	theme	bioactive	1753:1761	arg1	nanoparticles					1769:1781	copper-containing bioactive glass nanoparticles	1735:1781	copper-containing bioactive glass nanoparticles	1735:1781	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	4	65	theme	collagen	1141:1148	arg1	deposition					1150:1159	mineralized collagen deposition	1129:1159	mineralized collagen deposition	1129:1159	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	8	66	theme	novel	1691:1695	arg1	type					1697:1700	a novel type	1689:1700	a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite	1689:1817	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	10	67	theme	cell	2212:2215	arg1	processing					2217:2226	time consuming cell processing	2197:2226	time consuming cell processing	2197:2226	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	9	68	theme	injectability	1852:1864	arg1	properties					1907:1916	injectability and thermally triggered in situ gelation properties	1852:1916	injectability and thermally triggered in situ gelation properties	1852:1916	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	8	69	theme	chitosan/silk	1787:1799	arg1	composite					1809:1817	chitosan/silk fibroin composite	1787:1817	chitosan/silk fibroin composite	1787:1817	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	4	70	theme	bone	1113:1116	arg1	tissue					1118:1123	vascularized bone tissue	1100:1123	vascularized bone tissue	1100:1123	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	4	71	theme	bone	922:925	arg1	repair					927:932	In vivo bone repair	914:932	In vivo bone repair based on a critical-size rat calvarial bone defect model	914:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	0	72	from	formation	109:117	arg1	defects					141:147	rat calvarial bone defects	122:147	rat calvarial bone defects	122:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	9	73	from	release	1950:1956	arg1	doses					1988:1992	safe but effective doses	1969:1992	safe but effective doses	1969:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	10	74	theme	expensive	2246:2254	arg1	factors					2263:2269	expensive growth factors	2246:2269	expensive growth factors	2246:2269	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	9	75	theme	seeded	2036:2041	arg1	cells					2043:2047	the seeded cells	2032:2047	the seeded cells	2032:2047	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	3	76	theme	MC3T3-E1	780:787	arg1	growth					763:768	the growth	759:768	the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells	759:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	7	77	theme	clinic	1663:1668	arg1	translation					1670:1680	the clinic translation	1659:1680	the clinic translation	1659:1680	However, they have been facing obstacles related to the clinic translation.
30986530	10	78	theme	factors	2263:2269	arg1	use					2239:2241	the use	2235:2241	the use of expensive growth factors	2235:2269	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	5	79	theme	developed	1293:1301	arg1	hydrogels					1328:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	1	80	theme	Copper-containing	150:166	arg1	nanoparticles					184:196	Copper-containing bioactive glass nanoparticles	150:196	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes	150:245	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	80	theme	Copper-containing	150:166	arg1	NPs					205:207	Cu-BG NPs	199:207	Cu-BG NPs	199:207	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	81	theme	In	914:915	arg1	repair					927:932	In vivo bone repair	914:932	In vivo bone repair based on a critical-size rat calvarial bone defect model	914:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	4	82	theme	treatment	1170:1178	arg1	period					1180:1185	a treatment period	1168:1185	a treatment period of 8 weeks without utilization of any cells and/or growth factors	1168:1251	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	5	83	theme	composite	1318:1326	arg1	hydrogels					1328:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	the presently developed Cu-BG/CH/SF/GP composite hydrogels	1279:1336	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	3	84	theme	vein	809:812	arg1	cells					826:830	seeded MC3T3-E1 and human umbilical vein endothelial cells	773:830	cells	826:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	0	85	theme	in-situ	96:102	arg1	formation					109:117	in-situ bone formation	96:117	in-situ bone formation in rat calvarial bone defects	96:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	1	86	theme	glass	178:182	arg1	nanoparticles					184:196	Copper-containing bioactive glass nanoparticles	150:196	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes	150:245	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	1	86	theme	glass	178:182	arg1	NPs					205:207	Cu-BG NPs	199:207	Cu-BG NPs	199:207	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	4	87	theme	bone	973:976	arg1	model					985:989	a critical-size rat calvarial bone defect model	943:989	a critical-size rat calvarial bone defect model	943:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	3	88	theme	human	793:797	arg1	cells					826:830	seeded MC3T3-E1 and human umbilical vein endothelial cells	773:830	cells	826:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	2	89	theme	rapid	508:512	arg1	gelation					514:521	rapid gelation	508:521	rapid gelation	508:521	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	0	90	theme	rat	122:124	arg1	defects					141:147	rat calvarial bone defects	122:147	rat calvarial bone defects	122:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	2	91	theme	respective	649:658	arg1	doses					665:669	their respective safe doses	643:669	their respective safe doses	643:669	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	10	92	theme	consuming	2202:2210	arg1	processing					2217:2226	time consuming cell processing	2197:2226	time consuming cell processing	2197:2226	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	5	93	theme	translation	1363:1373	arg1	ability					1375:1381	translation ability	1363:1381	translation ability	1363:1381	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	3	94	theme	cells	826:830	arg1	growth					763:768	the growth	759:768	the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells	759:830	In vitro studies revealed that the gels supported the growth of seeded MC3T3-E1 and human umbilical vein endothelial cells, and effectively induced them toward osteogenesis and angiogenesis, respectively.
30986530	0	95	theme	bone	136:139	arg1	defects					141:147	rat calvarial bone defects	122:147	rat calvarial bone defects	122:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	4	96	theme	rat	959:961	arg1	model					985:989	a critical-size rat calvarial bone defect model	943:989	a critical-size rat calvarial bone defect model	943:989	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	4	97	theme	cells	1225:1229	arg1	utilization					1206:1216	utilization	1206:1216	utilization of any cells	1206:1229	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	9	98	theme	triggered	1880:1888	arg1	properties					1907:1916	injectability and thermally triggered in situ gelation properties	1852:1916	injectability and thermally triggered in situ gelation properties	1852:1916	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	0	99	theme	triggered	10:18	arg1	chitosan/silk					31:43	Thermally triggered injectable chitosan/silk	0:43	Thermally triggered injectable chitosan/silk	0:43	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	1	100	theme	cell-free	387:395	arg1	repair					402:407	cell-free bone repair	387:407	cell-free bone repair	387:407	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	9	101	theme	gelation	1898:1905	arg1	properties					1907:1916	injectability and thermally triggered in situ gelation properties	1852:1916	injectability and thermally triggered in situ gelation properties	1852:1916	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	4	102	theme	growth	1238:1243	arg1	factors					1245:1251	growth factors	1238:1251	growth factors	1238:1251	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	1	103	theme	fibroin	305:311	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	0	104	theme	glass	63:67	arg1	hydrogels					82:90	glass nanoparticle hydrogels	63:90	glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects	63:147	Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.
30986530	1	105	theme	SF	314:315	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	10	106	theme	critical-size	2138:2150	arg1	defects					2171:2177	critical-size rat calvarial bone defects	2138:2177	critical-size rat calvarial bone defects	2138:2177	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	5	107	theme	thermo-sensitive	1420:1435	arg1	properties					1437:1446	their thermo-sensitive properties	1414:1446	their thermo-sensitive properties	1414:1446	The results suggest that the presently developed Cu-BG/CH/SF/GP composite hydrogels have great potential and translation ability for bone regeneration owing to their thermo-sensitive properties, cell-free bioactivity, and cost-effectiveness.
30986530	2	108	theme	resulting	414:422	arg1	gels					439:442	The resulting Cu-BG/CH/SF/GP gels	410:442	The resulting Cu-BG/CH/SF/GP gels	410:442	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	1	109	theme	/glycerophosphate	317:333	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	6	110	dep	STATEMENT	1496:1504	arg1	Hydrogels					1523:1531	Hydrogels	1523:1531	Hydrogels	1523:1531	STATEMENT OF SIGNIFICANCE: Hydrogels loaded with cells and/or growth factors exhibit potential in bone repair.
30986530	4	111	theme	optimal	1013:1019	arg1	able					1044:1047	able	1044:1047	able	1044:1047	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	4	111	theme	optimal	1013:1019	arg1	gel					1036:1038	the optimal Cu-BG/CH/SF/GP gel	1009:1038	the optimal Cu-BG/CH/SF/GP gel	1009:1038	In vivo bone repair based on a critical-size rat calvarial bone defect model demonstrated that the optimal Cu-BG/CH/SF/GP gel was able to fully restore the bone defect with formation of vascularized bone tissue and mineralized collagen deposition during a treatment period of 8 weeks without utilization of any cells and/or growth factors.
30986530	8	112	theme	hydrogel	1705:1712	arg1	system					1714:1719	hydrogel system	1705:1719	hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite	1705:1817	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
30986530	1	113	theme	GP	336:337	arg1	composites					340:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites	286:349	Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair.
30986530	10	114	theme	calvarial	2156:2164	arg1	defects					2171:2177	critical-size rat calvarial bone defects	2138:2177	critical-size rat calvarial bone defects	2138:2177	The optimal gel showed the ability to fully repair critical-size rat calvarial bone defects without involving time consuming cell processing and/or the use of expensive growth factors, confirming that this novel hydrogel system has great potential for translation to the clinic.
30986530	2	115	theme	physiological	526:538	arg1	temperature					540:550	physiological temperature	526:550	physiological temperature	526:550	The resulting Cu-BG/CH/SF/GP gels were found to exhibit well-defined injectability and to undergo rapid gelation at physiological temperature and pH. They were highly porous and showed the ability to administer Si, Ca and Cu ions at their respective safe doses in a sustained and controlled manner.
30986530	9	116	theme	ions	1961:1964	arg1	release					1950:1956	the release	1946:1956	the release of ions at safe but effective doses	1946:1992	These gels showed injectability and thermally triggered in situ gelation properties and were able to administer the release of ions at safe but effective doses in a controlled manner while inducing the seeded cells toward osteogenesis and angiogenesis.
30986530	6	117	theme	SIGNIFICANCE	1509:1520	arg1	STATEMENT					1496:1504	STATEMENT	1496:1504	STATEMENT OF SIGNIFICANCE: Hydrogels loaded with cells and/or growth factors	1496:1571	STATEMENT OF SIGNIFICANCE: Hydrogels loaded with cells and/or growth factors exhibit potential in bone repair.
30986530	8	118	theme	copper-containing	1735:1751	arg1	nanoparticles					1769:1781	copper-containing bioactive glass nanoparticles	1735:1781	copper-containing bioactive glass nanoparticles	1735:1781	Here, a novel type of hydrogel system consisting of copper-containing bioactive glass nanoparticles and chitosan/silk fibroin composite was developed.
31783070	2	0	theme	rotatable	325:333	arg1	design					335:340	Central composite rotatable design	307:340	Central composite rotatable design of response surface methodology	307:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	1	1	theme	millet	254:259	arg1	PMS					269:271	PMS	269:271	PMS	269:271	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	1	1	theme	millet	254:259	arg1	starch					261:266	pearl millet starch	248:266	pearl millet starch (PMS)	248:272	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	4	2	theme	water	575:579	arg1	thickness					564:572	thickness	564:572	thickness	564:572	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	4	2	theme	water	575:579	arg1	WVP					601:603	WVP	601:603	WVP	601:603	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	4	2	theme	water	575:579	arg1	permeability					587:598	water vapor permeability	575:598	water vapor permeability (WVP)	575:604	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	10	3	theme	optimized	1503:1511	arg1	film					1520:1523	the developed optimized PMS/CG film	1489:1523	the developed optimized PMS/CG film	1489:1523	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	9	4	theme	Differential	1320:1331	arg1	analysis					1354:1361	Differential Scanning Calorimeter analysis	1320:1361	Differential Scanning Calorimeter analysis	1320:1361	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	7	5	theme	PMS/CG	1068:1073	arg1	film					1075:1078	optimized PMS/CG film	1058:1078	optimized PMS/CG film	1058:1078	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	2	6	theme	film	501:504	arg1	development					476:486	the development	472:486	the development of composite film	472:504	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	1	7	theme	present	186:192	arg1	study					194:198	The present study	182:198	The present study	182:198	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	8	8	theme	PMS	1163:1165	arg1	film					1167:1170	PMS film	1163:1170	PMS film	1163:1170	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	10	9	theme	lower	1535:1539	arg1	WVP					1541:1543	lower WVP	1535:1543	lower WVP	1535:1543	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	0	10	theme	carrageenan	108:118	arg1	gum					120:122	carrageenan gum	108:122	carrageenan gum	108:122	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	9	11	theme	Scanning	1333:1340	arg1	analysis					1354:1361	Differential Scanning Calorimeter analysis	1320:1361	Differential Scanning Calorimeter analysis	1320:1361	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	9	12	theme	different	1414:1422	arg1	components					1424:1433	different components	1414:1433	different components of PMS/CG composite films	1414:1459	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	8	13	theme	Morphological	1106:1118	arg1	studies					1120:1126	Morphological studies	1106:1126	Morphological studies	1106:1126	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	8	14	theme	composite	1185:1193	arg1	film					1195:1198	PMS/CG composite film	1178:1198	PMS/CG composite film	1178:1198	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	6	15	theme	different	827:835	arg1	parameters					837:846	different parameters	827:846	different parameters resulting in a composite film with maximum TS and lower WVP and solubility values	827:928	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	2	16	theme	composite	491:499	arg1	film					501:504	composite film	491:504	composite film	491:504	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	0	17	theme	response	152:159	arg1	methodology					169:179	response surface methodology	152:179	response surface methodology	152:179	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	9	18	theme	composite	1445:1453	arg1	films					1455:1459	PMS/CG composite films	1438:1459	PMS/CG composite films	1438:1459	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	6	19	theme	solubility	912:921	arg1	values					923:928	lower WVP and solubility values	898:928	values	923:928	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	9	20	theme	Calorimeter	1342:1352	arg1	analysis					1354:1361	Differential Scanning Calorimeter analysis	1320:1361	Differential Scanning Calorimeter analysis	1320:1361	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	2	21	used	used	378:381	arg2	design					335:340	Central composite rotatable design	307:340	Central composite rotatable design of response surface methodology	307:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	5	22	theme	carrageenan	661:671	arg1	concentrations					673:686	Both starch and carrageenan concentrations	645:686	Both starch and carrageenan concentrations	645:686	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	8	23	theme	irregular	1232:1240	arg1	surface					1242:1248	rough, coarser and slightly irregular surface	1204:1248	rough, coarser and slightly irregular surface	1204:1248	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	0	24	theme	pearl	84:88	arg1	starch					97:102	pearl millet starch	84:102	pearl millet starch	84:102	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	6	25	theme	maximum	883:889	arg1	TS					891:892	maximum TS	883:892	maximum TS	883:892	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	6	26	theme	%	967:967	arg1	concentrations					1015:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	2	27	theme	methodology	362:372	arg1	design					335:340	Central composite rotatable design	307:340	Central composite rotatable design of response surface methodology	307:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	0	28	theme	Physical	0:7	arg1	properties					33:42	Physical, structural and thermal properties	0:42	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	0	29	theme	structural	10:19	arg1	properties					33:42	Physical, structural and thermal properties	0:42	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	10	30	contain	possessed	1525:1533	arg2	solubility					1546:1555	solubility	1546:1555	solubility	1546:1555	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	10	30	contain	possessed	1525:1533	arg2	higher					1561:1566	higher	1561:1566	higher	1561:1566	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	10	30	contain	possessed	1525:1533	arg1	film					1520:1523	the developed optimized PMS/CG film	1489:1523	the developed optimized PMS/CG film	1489:1523	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	10	30	contain	possessed	1525:1533	arg2	WVP					1541:1543	lower WVP	1535:1543	lower WVP	1535:1543	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	0	31	theme	edible	57:62	arg1	films					64:68	composite edible films	47:68	composite edible films prepared from pearl millet starch and carrageenan gum	47:122	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	9	32	dep	miscibility	1377:1387	arg1	the					1373:1375	the	1373:1375	the	1373:1375	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	7	33	theme	melting	1035:1041	arg1	higher					1084:1089	higher	1084:1089	higher	1084:1089	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	7	33	theme	melting	1035:1041	arg1	temperature					1043:1053	The melting temperature	1031:1053	The melting temperature of optimized PMS/CG film	1031:1078	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	1	34	theme	gum	290:292	arg1	blends					299:304	pearl millet starch (PMS) and carrageenan gum (CG) blends	248:304	pearl millet starch (PMS) and carrageenan gum (CG) blends	248:304	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	0	35	theme	thermal	25:31	arg1	properties					33:42	Physical, structural and thermal properties	0:42	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	5	36	theme	starch	650:655	arg1	concentrations					673:686	Both starch and carrageenan concentrations	645:686	Both starch and carrageenan concentrations	645:686	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	2	37	theme	composite	315:323	arg1	design					335:340	Central composite rotatable design	307:340	Central composite rotatable design of response surface methodology	307:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	6	38	theme	%	1001:1001	arg1	plasticizer					1003:1013	15.73% plasticizer	996:1013	15.73% plasticizer	996:1013	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	3	39	dep	responses	549:557	arg1	viz					559:561	viz	559:561	responses viz	549:561	The optimization was done on the basis of responses viz.
31783070	9	40	theme	Fourier-transform	1276:1292	arg1	spectroscopy					1303:1314	Fourier-transform infrared spectroscopy	1276:1314	Fourier-transform infrared spectroscopy	1276:1314	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	10	41	theme	developed	1493:1501	arg1	film					1520:1523	the developed optimized PMS/CG film	1489:1523	the developed optimized PMS/CG film	1489:1523	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	2	42	theme	response	345:352	arg1	methodology					362:372	response surface methodology	345:372	response surface methodology	345:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	0	43	theme	composite	47:55	arg1	films					64:68	composite edible films	47:68	composite edible films prepared from pearl millet starch and carrageenan gum	47:122	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	6	44	theme	%	978:978	arg1	concentrations					1015:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	10	45	dep	higher	1561:1566	arg1	TS					1585:1586	TS	1585:1586	TS	1585:1586	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	10	45	dep	higher	1561:1566	arg1	transparency					1568:1579	transparency	1568:1579	transparency	1568:1579	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	1	46	theme	pearl	248:252	arg1	PMS					269:271	PMS	269:271	PMS	269:271	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	1	46	theme	pearl	248:252	arg1	starch					261:266	pearl millet starch	248:266	pearl millet starch (PMS)	248:272	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	6	47	theme	optimum	810:816	arg1	level					818:822	The optimum level	806:822	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values	806:928	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	1	48	theme	starch	261:266	arg1	blends					299:304	pearl millet starch (PMS) and carrageenan gum (CG) blends	248:304	pearl millet starch (PMS) and carrageenan gum (CG) blends	248:304	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	10	49	theme	PMS/CG	1513:1518	arg1	film					1520:1523	the developed optimized PMS/CG film	1489:1523	the developed optimized PMS/CG film	1489:1523	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	6	50	with	film	873:876	arg1	TS					891:892	maximum TS	883:892	maximum TS	883:892	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	6	50	with	film	873:876	arg1	WVP					904:906	lower WVP and solubility values	898:928	WVP	904:906	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	8	51	theme	homogeneous	1138:1148	arg1	structure					1150:1158	homogeneous structure	1138:1158	homogeneous structure of PMS film	1138:1170	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	1	52	theme	edible	220:225	arg1	films					237:241	edible composite films	220:241	edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends	220:304	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	9	53	theme	thermal	1393:1399	arg1	stability					1401:1409	thermal stability	1393:1409	thermal stability	1393:1409	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	6	54	theme	composite	863:871	arg1	film					873:876	a composite film	861:876	a composite film with maximum TS and lower WVP and solubility values	861:928	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	6	55	theme	concentrations	1015:1028	arg1	conditions					950:959	conditions	950:959	conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	950:1028	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	0	56	theme	Process	125:131	arg1	optimization					133:144	Process optimization	125:144	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	4	57	theme	vapor	581:585	arg1	thickness					564:572	thickness	564:572	thickness	564:572	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	4	57	theme	vapor	581:585	arg1	WVP					601:603	WVP	601:603	WVP	601:603	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	4	57	theme	vapor	581:585	arg1	permeability					587:598	water vapor permeability	575:598	water vapor permeability (WVP)	575:604	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	5	58	theme	reverse	737:743	arg1	trend					745:749	the reverse trend	733:749	the reverse trend	733:749	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	8	59	theme	film	1167:1170	arg1	structure					1150:1158	homogeneous structure	1138:1158	homogeneous structure of PMS film	1138:1170	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	9	60	theme	components	1424:1433	arg1	miscibility					1377:1387	miscibility	1377:1387	miscibility	1377:1387	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	9	60	theme	components	1424:1433	arg1	stability					1401:1409	thermal stability	1393:1409	thermal stability	1393:1409	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	6	61	theme	parameters	837:846	arg1	level					818:822	The optimum level	806:822	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values	806:928	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	8	62	contain	had	1200:1202	arg1	film					1195:1198	PMS/CG composite film	1178:1198	PMS/CG composite film	1178:1198	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	8	62	contain	had	1200:1202	arg2	surface					1242:1248	rough, coarser and slightly irregular surface	1204:1248	rough, coarser and slightly irregular surface	1204:1248	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	8	63	theme	PMS/CG	1178:1183	arg1	film					1195:1198	PMS/CG composite film	1178:1198	PMS/CG composite film	1178:1198	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	0	64	theme	surface	161:167	arg1	methodology					169:179	response surface methodology	152:179	response surface methodology	152:179	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	9	65	theme	PMS/CG	1438:1443	arg1	films					1455:1459	PMS/CG composite films	1438:1459	PMS/CG composite films	1438:1459	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	5	66	theme	increased	769:777	arg1	concentration					779:791	increased concentration	769:791	increased concentration of glycerol	769:803	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	4	67	theme	tensile	622:628	arg1	thickness					564:572	thickness	564:572	thickness	564:572	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	4	67	theme	tensile	622:628	arg1	strength					630:637	tensile strength	622:637	tensile strength	622:637	thickness, water vapor permeability (WVP), solubility and tensile strength (TS).
31783070	0	68	theme	films	64:68	arg1	properties					33:42	Physical, structural and thermal properties	0:42	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	9	69	theme	films	1455:1459	arg1	components					1424:1433	different components	1414:1433	different components of PMS/CG composite films	1414:1459	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	8	70	theme	rough	1204:1208	arg1	surface					1242:1248	rough, coarser and slightly irregular surface	1204:1248	rough, coarser and slightly irregular surface	1204:1248	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	7	71	theme	PMS	1096:1098	arg1	film					1100:1103	PMS film	1096:1103	PMS film	1096:1103	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	9	72	from	regions	1265:1271	arg1	spectroscopy					1303:1314	Fourier-transform infrared spectroscopy	1276:1314	Fourier-transform infrared spectroscopy	1276:1314	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	9	72	from	regions	1265:1271	arg1	analysis					1354:1361	Differential Scanning Calorimeter analysis	1320:1361	Differential Scanning Calorimeter analysis	1320:1361	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	5	73	theme	glycerol	796:803	arg1	concentration					779:791	increased concentration	769:791	increased concentration of glycerol	769:803	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	6	74	theme	lower	898:902	arg1	WVP					904:906	lower WVP and solubility values	898:928	WVP	904:906	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	8	75	theme	coarser	1211:1217	arg1	surface					1242:1248	rough, coarser and slightly irregular surface	1204:1248	rough, coarser and slightly irregular surface	1204:1248	Morphological studies exhibited homogeneous structure of PMS film while PMS/CG composite film had rough, coarser and slightly irregular surface.
31783070	0	76	theme	millet	90:95	arg1	starch					97:102	pearl millet starch	84:102	pearl millet starch	84:102	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	7	77	theme	film	1075:1078	arg1	higher					1084:1089	higher	1084:1089	higher	1084:1089	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	7	77	theme	film	1075:1078	arg1	temperature					1043:1053	The melting temperature	1031:1053	The melting temperature of optimized PMS/CG film	1031:1078	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	2	78	theme	glycerol	450:457	arg1	concentrations					395:408	concentrations	395:408	concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%)	395:466	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	9	79	theme	infrared	1294:1301	arg1	spectroscopy					1303:1314	Fourier-transform infrared spectroscopy	1276:1314	Fourier-transform infrared spectroscopy	1276:1314	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	7	80	theme	optimized	1058:1066	arg1	film					1075:1078	optimized PMS/CG film	1058:1078	optimized PMS/CG film	1058:1078	The melting temperature of optimized PMS/CG film was higher than PMS film.
31783070	2	81	theme	surface	354:360	arg1	methodology					362:372	response surface methodology	345:372	response surface methodology	345:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	5	82	theme	films	718:722	arg1	TS					712:713	the TS	708:713	the TS of films	708:722	Both starch and carrageenan concentrations positively affected the TS of films, whereas the reverse trend was observed with increased concentration of glycerol.
31783070	2	83	theme	PMS	413:415	arg1	concentrations					395:408	concentrations	395:408	concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%)	395:466	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	6	84	dep	%	967:967	arg1	starch					969:974	starch	969:974	4.9% starch	964:974	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	1	85	theme	carrageenan	278:288	arg1	CG					295:296	CG	295:296	CG	295:296	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	1	85	theme	carrageenan	278:288	arg1	gum					290:292	carrageenan gum	278:292	carrageenan gum (CG)	278:297	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	2	86	theme	carrageenan	425:435	arg1	concentrations					395:408	concentrations	395:408	concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%)	395:466	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	3	87	theme	responses	549:557	arg1	basis					540:544	the basis	536:544	the basis of responses viz	536:561	The optimization was done on the basis of responses viz.
31783070	10	88	theme	PMS	1605:1607	arg1	film					1609:1612	PMS film	1605:1612	PMS film	1605:1612	The results indicated that the developed optimized PMS/CG film possessed lower WVP, solubility but higher transparency and TS in comparison to PMS film.
31783070	1	89	from	blends	299:304	arg1	films					237:241	edible composite films	220:241	edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends	220:304	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	9	90	theme	stretched	1255:1263	arg1	regions					1265:1271	The stretched regions	1251:1271	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis	1251:1361	The stretched regions in Fourier-transform infrared spectroscopy and Differential Scanning Calorimeter analysis confirmed the miscibility and thermal stability of different components of PMS/CG composite films.
31783070	0	91	dep	properties	33:42	arg1	optimization					133:144	Process optimization	125:144	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.	0:180	Physical, structural and thermal properties of composite edible films prepared from pearl millet starch and carrageenan gum: Process optimization using response surface methodology.
31783070	6	92	theme	plasticizer	1003:1013	arg1	concentrations					1015:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations	964:1028	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	2	93	theme	Central	307:313	arg1	design					335:340	Central composite rotatable design	307:340	Central composite rotatable design of response surface methodology	307:372	Central composite rotatable design of response surface methodology was used to optimize concentrations of PMS (2-5%), carrageenan (1-3.5%) and glycerol (15-35%) for the development of composite film.
31783070	6	94	theme	15.73	996:1000	arg1	%					1001:1001	%	1001:1001	%	1001:1001	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31783070	1	95	theme	composite	227:235	arg1	films					237:241	edible composite films	220:241	edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends	220:304	The present study aimed at developing edible composite films from pearl millet starch (PMS) and carrageenan gum (CG) blends.
31783070	6	96	theme	carrageenan	980:990	arg1	%					978:978	5% carrageenan	977:990	5% carrageenan	977:990	The optimum level of different parameters resulting in a composite film with maximum TS and lower WVP and solubility values were obtained under conditions of 4.9% starch, 5% carrageenan and 15.73% plasticizer concentrations.
31790572	0	0	theme	Cellulose-Water	79:93	arg1	Interfaces					95:104	Cellulose-Water Interfaces	79:104	Cellulose-Water Interfaces	79:104	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	5	1	theme	cellulose/water	780:794	arg1	interface					796:804	the cellulose/water interface	776:804	the cellulose/water interface induced by the increase in XG concentration close to the surface	776:869	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	7	2	theme	vital	1132:1136	arg1	importance					1138:1147	vital importance	1132:1147	vital importance	1132:1147	The study is of vital importance for improving the functionality of sustainable materials made from xyloglucan/cellulose natural composites.
31790572	4	3	theme	cellulose	598:606	arg1	surfaces					614:621	cellulose model surfaces	598:621	cellulose model surfaces using a quartz crystal microbalance with dissipation	598:674	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	5	4	from	increase	821:828	arg1	concentration					836:848	XG concentration	833:848	XG concentration close to the surface	833:869	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	6	5	theme	XG	930:931	arg1	fractions					933:941	the XG fractions	926:941	the XG fractions depending on the solubility properties	926:980	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	3	6	dep	molecules	377:385	arg1	molecules					377:385	the XG molecules and their association and phase separation	370:428	the XG molecules and their association and phase separation	370:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	6	dep	molecules	377:385	arg1	association					397:407	their association	391:407	their association	391:407	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	6	dep	molecules	377:385	arg1	separation					419:428	phase separation	413:428	phase separation	413:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	7	7	theme	natural	1237:1243	arg1	composites					1245:1254	xyloglucan/cellulose natural composites	1216:1254	xyloglucan/cellulose natural composites	1216:1254	The study is of vital importance for improving the functionality of sustainable materials made from xyloglucan/cellulose natural composites.
31790572	0	8	from	Influence	0:8	arg1	Adsorption					31:40	the Adsorption	27:40	the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces	27:104	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	7	9	theme	xyloglucan/cellulose	1216:1235	arg1	composites					1245:1254	xyloglucan/cellulose natural composites	1216:1254	xyloglucan/cellulose natural composites	1216:1254	The study is of vital importance for improving the functionality of sustainable materials made from xyloglucan/cellulose natural composites.
31790572	6	10	from	separation	1040:1049	arg1	media					1069:1073	aqueous media	1061:1073	aqueous media	1061:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	2	11	theme	light	279:283	arg1	scattering					285:294	light scattering	279:294	light scattering	279:294	The solubility of the fractions was investigated using light scattering, chromatography, and microscopy techniques.
31790572	5	12	dep	formation	722:730	arg1	a					712:712	a	712:712	a	712:712	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	4	13	theme	model	608:612	arg1	surfaces					614:621	cellulose model surfaces	598:621	cellulose model surfaces using a quartz crystal microbalance with dissipation	598:674	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	3	14	theme	conformational	344:357	arg1	changes					359:365	The conformational changes	340:365	The conformational changes of the XG molecules and their association and phase separation	340:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	1	15	with	fractions	122:130	arg1	masses					153:158	different molar masses	137:158	different molar masses	137:158	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	6	16	theme	aqueous	1061:1067	arg1	media					1069:1073	aqueous media	1061:1073	aqueous media	1061:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	5	17	theme	molecules	763:771	arg1	formation					722:730	cluster formation	714:730	cluster formation	714:730	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	5	17	theme	molecules	763:771	arg1	separation					742:751	phase separation	736:751	phase separation	736:751	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	6	18	from	insolubility	1003:1014	arg1	media					1069:1073	aqueous media	1061:1073	aqueous media	1061:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	5	19	theme	XG	760:761	arg1	molecules					763:771	the XG molecules	756:771	the XG molecules	756:771	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	7	20	theme	materials	1196:1204	arg1	functionality					1167:1179	the functionality	1163:1179	the functionality of sustainable materials made from xyloglucan/cellulose natural composites	1163:1254	The study is of vital importance for improving the functionality of sustainable materials made from xyloglucan/cellulose natural composites.
31790572	6	21	from	fractions	933:941	arg1	properties					971:980	the solubility properties	956:980	the solubility properties	956:980	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	22	from	XGs	1054:1056	arg1	media					1069:1073	aqueous media	1061:1073	aqueous media	1061:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	5	23	theme	XG	833:834	arg1	concentration					836:848	XG concentration	833:848	XG concentration close to the surface	833:869	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	4	24	theme	different	572:580	arg1	fractions					585:593	different XG fractions	572:593	different XG fractions	572:593	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	4	25	with	microbalance	646:657	arg1	dissipation					664:674	dissipation	664:674	dissipation	664:674	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	6	26	from	media	1069:1073	arg1	association					1017:1027	association	1017:1027	association	1017:1027	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	26	from	media	1069:1073	arg1	insolubility					1003:1014	the insolubility	999:1014	the insolubility	999:1014	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	26	from	media	1069:1073	arg1	separation					1040:1049	phase separation	1034:1049	phase separation of XGs in aqueous media	1034:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	3	27	theme	XG	374:375	arg1	molecules					377:385	the XG molecules and their association and phase separation	370:428	the XG molecules and their association and phase separation	370:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	27	theme	XG	374:375	arg1	association					397:407	their association	391:407	their association	391:407	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	27	theme	XG	374:375	arg1	separation					419:428	phase separation	413:428	phase separation	413:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	1	28	theme	Xylogucan	107:115	arg1	fractions					122:130	Xylogucan (XG) fractions	107:130	Xylogucan (XG) fractions with different molar masses	107:158	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	3	29	theme	molar	480:484	arg1	changes					491:497	molar mass changes	480:497	molar mass changes	480:497	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	30	theme	molecules	377:385	arg1	changes					359:365	The conformational changes	340:365	The conformational changes of the XG molecules and their association and phase separation	340:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	4	31	theme	fractions	585:593	arg1	interaction					557:567	the interaction	553:567	the interaction of different XG fractions	553:593	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	6	32	theme	adsorption	891:900	arg1	regimes					902:908	the adsorption regimes	887:908	the adsorption regimes	887:908	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	3	33	theme	mass	486:489	arg1	changes					491:497	molar mass changes	480:497	molar mass changes	480:497	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	34	theme	phase	413:417	arg1	molecules					377:385	the XG molecules and their association and phase separation	370:428	the XG molecules and their association and phase separation	370:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	3	34	theme	phase	413:417	arg1	separation					419:428	phase separation	413:428	phase separation	413:428	The conformational changes of the XG molecules and their association and phase separation were investigated together with concentration and molar mass changes.
31790572	4	35	theme	XG	582:583	arg1	fractions					585:593	different XG fractions	572:593	different XG fractions	572:593	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	5	36	theme	phase	736:740	arg1	separation					742:751	phase separation	736:751	phase separation	736:751	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	6	37	theme	phase	1034:1038	arg1	separation					1040:1049	phase separation	1034:1049	phase separation of XGs in aqueous media	1034:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	38	theme	XGs	1054:1056	arg1	association					1017:1027	association	1017:1027	association	1017:1027	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	38	theme	XGs	1054:1056	arg1	insolubility					1003:1014	the insolubility	999:1014	the insolubility	999:1014	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	38	theme	XGs	1054:1056	arg1	separation					1040:1049	phase separation	1034:1049	phase separation of XGs in aqueous media	1034:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	0	39	theme	Different	45:53	arg1	Fractions					66:74	Different Xyloglucan Fractions	45:74	Different Xyloglucan Fractions	45:74	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	6	40	theme	solubility	960:969	arg1	properties					971:980	the solubility properties	956:980	the solubility properties	956:980	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	6	41	with	interaction	1088:1098	arg1	cellulose					1105:1113	cellulose	1105:1113	cellulose	1105:1113	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	1	42	theme	XG	118:119	arg1	fractions					122:130	Xylogucan (XG) fractions	107:130	Xylogucan (XG) fractions with different molar masses	107:158	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	6	43	from	association	1017:1027	arg1	media					1069:1073	aqueous media	1061:1073	aqueous media	1061:1073	Concomitantly, the adsorption regimes are altered for the XG fractions depending on the solubility properties, indicating that the insolubility, association, and phase separation of XGs in aqueous media affect their interaction with cellulose.
31790572	7	44	theme	sustainable	1184:1194	arg1	materials					1196:1204	sustainable materials	1184:1204	sustainable materials made from xyloglucan/cellulose natural composites	1184:1254	The study is of vital importance for improving the functionality of sustainable materials made from xyloglucan/cellulose natural composites.
31790572	5	45	from	interface	796:804	arg1	formation					722:730	cluster formation	714:730	cluster formation	714:730	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	5	45	from	interface	796:804	arg1	separation					742:751	phase separation	736:751	phase separation	736:751	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	1	46	theme	XG	220:221	arg1	structure					203:211	the natural structure	191:211	the natural structure of the XG	191:221	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	5	47	theme	cluster	714:720	arg1	formation					722:730	cluster formation	714:730	cluster formation	714:730	The results indicate that there is a cluster formation and phase separation of the XG molecules at the cellulose/water interface induced by the increase in XG concentration close to the surface.
31790572	2	48	theme	fractions	246:254	arg1	solubility					228:237	The solubility	224:237	The solubility of the fractions	224:254	The solubility of the fractions was investigated using light scattering, chromatography, and microscopy techniques.
31790572	2	49	theme	microscopy	317:326	arg1	techniques					328:337	microscopy techniques	317:337	microscopy techniques	317:337	The solubility of the fractions was investigated using light scattering, chromatography, and microscopy techniques.
31790572	0	50	theme	Fractions	66:74	arg1	Adsorption					31:40	the Adsorption	27:40	the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces	27:104	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	0	51	from	Interfaces	95:104	arg1	Adsorption					31:40	the Adsorption	27:40	the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces	27:104	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	1	52	theme	natural	195:201	arg1	structure					203:211	the natural structure	191:211	the natural structure of the XG	191:221	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	1	53	theme	different	137:145	arg1	masses					153:158	different molar masses	137:158	different molar masses	137:158	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	4	54	theme	crystal	638:644	arg1	microbalance					646:657	a quartz crystal microbalance	629:657	a quartz crystal microbalance with dissipation	629:674	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
31790572	0	55	theme	Xyloglucan	55:64	arg1	Fractions					66:74	Different Xyloglucan Fractions	45:74	Different Xyloglucan Fractions	45:74	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	0	56	theme	Solubility	13:22	arg1	Influence					0:8	Influence	0:8	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.	0:105	Influence of Solubility on the Adsorption of Different Xyloglucan Fractions at Cellulose-Water Interfaces.
31790572	1	57	theme	molar	147:151	arg1	masses					153:158	different molar masses	137:158	different molar masses	137:158	Xylogucan (XG) fractions with different molar masses were prepared while preserving the natural structure of the XG.
31790572	4	58	theme	quartz	631:636	arg1	microbalance					646:657	a quartz crystal microbalance	629:657	a quartz crystal microbalance with dissipation	629:674	The knowledge gained was then applied to investigate the interaction of different XG fractions at cellulose model surfaces using a quartz crystal microbalance with dissipation.
29920370	1	0	theme	Surface	148:154	arg1	acylation					156:164	Surface acylation	148:164	Surface acylation of microcrystalline cellulose (MCC)	148:200	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	10	1	theme	human	1227:1231	arg1	HDF					1252:1254	HDF	1252:1254	HDF	1252:1254	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	10	1	theme	human	1227:1231	arg1	fibroblast					1240:1249	human dermal fibroblast	1227:1249	human dermal fibroblast (HDF) monolayer culture	1227:1273	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	0	2	theme	lactic	74:79	arg1	poly					69:72	poly	69:72	poly(lactic acid)	69:85	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	0	2	theme	lactic	74:79	arg1	acid					81:84	lactic acid	74:84	lactic acid	74:84	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	8	3	theme	composites	1017:1026	arg1	surfaces					1001:1008	The fractured surfaces	987:1008	The fractured surfaces of the composites	987:1026	The fractured surfaces of the composites showed an even distribution of RAMCC throughout PLA matrix.
29920370	9	4	theme	soil	1140:1143	arg1	method					1152:1157	soil buried method	1140:1157	soil buried method	1140:1157	Biodegradability of samples was characterized using soil buried method.
29920370	11	5	theme	sustainable	1446:1456	arg1	utilization					1458:1468	a sustainable utilization	1444:1468	a sustainable utilization of RBO and MCC in the packaging application	1444:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	5	theme	sustainable	1446:1456	arg1	films					1431:1435	These biodegradable composite films	1401:1435	These biodegradable composite films	1401:1435	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	6	theme	RBO	1473:1475	arg1	utilization					1458:1468	a sustainable utilization	1444:1468	a sustainable utilization of RBO and MCC in the packaging application	1444:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	6	theme	RBO	1473:1475	arg1	films					1431:1435	These biodegradable composite films	1401:1435	These biodegradable composite films	1401:1435	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	4	7	theme	mechanical	535:544	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	5	8	theme	water	683:687	arg1	sorption					689:696	lower water sorption	677:696	lower water sorption	677:696	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	5	9	theme	PLA/RAMCC	623:631	arg1	loading					658:664	with 2 wt% loading	647:664	with 2 wt% loading	647:664	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	5	9	theme	PLA/RAMCC	623:631	arg1	biocomposite					633:644	PLA/RAMCC biocomposite	623:644	PLA/RAMCC biocomposite (with 2 wt% loading)	623:665	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	9	10	theme	buried	1145:1150	arg1	method					1152:1157	soil buried method	1140:1157	soil buried method	1140:1157	Biodegradability of samples was characterized using soil buried method.
29920370	3	11	theme	Attenuated	327:336	arg1	Fourier					362:368	Attenuated Total Reflection (ATR)- Fourier	327:368	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy	327:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	6	12	theme	vapour	844:849	arg1	permeability					851:862	water vapour permeability	838:862	water vapour permeability	838:862	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	2	13	theme	acylated	257:264	arg1	RAMCC					271:275	RAMCC	271:275	RAMCC	271:275	The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC.
29920370	2	13	theme	acylated	257:264	arg1	MCC					266:268	The resultant acylated MCC	243:268	The resultant acylated MCC (RAMCC)	243:276	The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC.
29920370	4	14	theme	thermal	547:553	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	0	15	theme	microcrystalline	100:115	arg1	cellulose					117:125	acylated microcrystalline cellulose	91:125	acylated microcrystalline cellulose	91:125	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	6	16	theme	water	838:842	arg1	permeability					851:862	water vapour permeability	838:862	water vapour permeability	838:862	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	4	17	theme	PLA	585:587	arg1	biocomposite					595:606	the PLA based biocomposite	581:606	the PLA based biocomposite	581:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	2	18	theme	resultant	247:255	arg1	RAMCC					271:275	RAMCC	271:275	RAMCC	271:275	The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC.
29920370	2	18	theme	resultant	247:255	arg1	MCC					266:268	The resultant acylated MCC	243:268	The resultant acylated MCC (RAMCC)	243:276	The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC.
29920370	3	19	theme	infrared	380:387	arg1	spectroscopy					396:407	infrared (FTIR) spectroscopy	380:407	infrared (FTIR) spectroscopy	380:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	0	20	theme	acylated	91:98	arg1	cellulose					117:125	acylated microcrystalline cellulose	91:125	acylated microcrystalline cellulose	91:125	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	11	21	theme	MCC	1481:1483	arg1	utilization					1458:1468	a sustainable utilization	1444:1468	a sustainable utilization of RBO and MCC in the packaging application	1444:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	21	theme	MCC	1481:1483	arg1	films					1431:1435	These biodegradable composite films	1401:1435	These biodegradable composite films	1401:1435	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	4	22	theme	barrier	559:565	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	1	23	theme	microcrystalline	169:184	arg1	cellulose					186:194	microcrystalline cellulose	169:194	microcrystalline cellulose (MCC)	169:200	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	1	23	theme	microcrystalline	169:184	arg1	MCC					197:199	MCC	197:199	MCC	197:199	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	10	24	theme	monolayer	1257:1265	arg1	culture					1267:1273	human dermal fibroblast (HDF) monolayer culture	1227:1273	human dermal fibroblast (HDF) monolayer culture	1227:1273	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	3	25	dep	Fourier	362:368	arg1	transform					370:378	transform	370:378	transform infrared (FTIR) spectroscopy	370:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	6	26	theme	Diffraction	738:748	arg1	analysis					756:763	X-ray Diffraction (XRD) analysis result	732:770	X-ray Diffraction (XRD) analysis result	732:770	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	6	26	theme	Diffraction	738:748	arg1	XRD					751:753	XRD	751:753	XRD	751:753	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	10	27	theme	fibroblast	1240:1249	arg1	culture					1267:1273	human dermal fibroblast (HDF) monolayer culture	1227:1273	human dermal fibroblast (HDF) monolayer culture	1227:1273	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	1	28	theme	cellulose	186:194	arg1	acylation					156:164	Surface acylation	148:164	Surface acylation of microcrystalline cellulose (MCC)	148:200	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	3	29	theme	ATR	356:358	arg1	Fourier					362:368	Attenuated Total Reflection (ATR)- Fourier	327:368	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy	327:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	6	30	theme	X-ray	732:736	arg1	Diffraction					738:748	X-ray Diffraction	732:748	X-ray Diffraction (XRD) analysis result	732:770	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	10	31	theme	dermal	1233:1238	arg1	HDF					1252:1254	HDF	1252:1254	HDF	1252:1254	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	10	31	theme	dermal	1233:1238	arg1	fibroblast					1240:1249	human dermal fibroblast	1227:1249	human dermal fibroblast (HDF) monolayer culture	1227:1273	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	3	32	theme	Total	338:342	arg1	Fourier					362:368	Attenuated Total Reflection (ATR)- Fourier	327:368	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy	327:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	9	33	theme	samples	1108:1114	arg1	Biodegradability					1088:1103	Biodegradability	1088:1103	Biodegradability of samples	1088:1114	Biodegradability of samples was characterized using soil buried method.
29920370	11	34	theme	packaging	1492:1500	arg1	application					1502:1512	the packaging application	1488:1512	the packaging application	1488:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	35	from	utilization	1458:1468	arg1	application					1502:1512	the packaging application	1488:1512	the packaging application	1488:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	3	36	theme	hydrophobic	419:429	arg1	modification					435:446	hydrophobic MCC modification	419:446	hydrophobic MCC modification	419:446	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	3	37	theme	Reflection	344:353	arg1	Fourier					362:368	Attenuated Total Reflection (ATR)- Fourier	327:368	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy	327:407	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	5	38	theme	lower	677:681	arg1	sorption					689:696	lower water sorption	677:696	lower water sorption	677:696	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	11	39	theme	composite	1421:1429	arg1	utilization					1458:1468	a sustainable utilization	1444:1468	a sustainable utilization of RBO and MCC in the packaging application	1444:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	39	theme	composite	1421:1429	arg1	films					1431:1435	These biodegradable composite films	1401:1435	These biodegradable composite films	1401:1435	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	3	40	theme	MCC	431:433	arg1	modification					435:446	hydrophobic MCC modification	419:446	hydrophobic MCC modification	419:446	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	0	41	theme	packaging	49:57	arg1	film					59:62	biocomposite packaging film	36:62	biocomposite packaging film	36:62	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	4	42	theme	morphological	520:532	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	0	43	theme	bran	138:141	arg1	oil					143:145	rice bran oil	133:145	rice bran oil	133:145	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	6	44	theme	PLA/RAMCC	811:819	arg1	crystallinity					794:806	crystallinity	794:806	crystallinity of PLA/RAMCC	794:819	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	3	45	dep	infrared	380:387	arg1	FTIR					390:393	FTIR	390:393	FTIR	390:393	Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification.
29920370	6	46	dep	analysis	756:763	arg1	result					765:770	result	765:770	result	765:770	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	6	47	from	reduction	825:833	arg1	crystallinity					794:806	crystallinity	794:806	crystallinity of PLA/RAMCC	794:819	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	6	47	from	reduction	825:833	arg1	permeability					851:862	water vapour permeability	838:862	water vapour permeability	838:862	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	0	48	theme	biocomposite	36:47	arg1	film					59:62	biocomposite packaging film	36:62	biocomposite packaging film	36:62	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	6	49	theme	PLA/MCC	892:898	arg1	composite					900:908	PLA/MCC composite	892:908	PLA/MCC composite	892:908	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	0	50	theme	rice	133:136	arg1	oil					143:145	rice bran oil	133:145	rice bran oil	133:145	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	11	51	theme	biodegradable	1407:1419	arg1	utilization					1458:1468	a sustainable utilization	1444:1468	a sustainable utilization of RBO and MCC in the packaging application	1444:1512	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	11	51	theme	biodegradable	1407:1419	arg1	films					1431:1435	These biodegradable composite films	1401:1435	These biodegradable composite films	1401:1435	These biodegradable composite films can be a sustainable utilization of RBO and MCC in the packaging application.
29920370	6	52	from	increase	782:789	arg1	crystallinity					794:806	crystallinity	794:806	crystallinity of PLA/RAMCC	794:819	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	6	52	from	increase	782:789	arg1	permeability					851:862	water vapour permeability	838:862	water vapour permeability	838:862	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	4	53	theme	biocomposite	595:606	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	4	54	theme	PLA	486:488	arg1	matrix					490:495	PLA matrix	486:495	PLA matrix	486:495	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	8	55	theme	RAMCC	1059:1063	arg1	distribution					1043:1054	an even distribution	1035:1054	an even distribution of RAMCC	1035:1063	The fractured surfaces of the composites showed an even distribution of RAMCC throughout PLA matrix.
29920370	0	56	from	poly	69:72	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	0	56	from	poly	69:72	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	7	57	theme	barrier	967:973	arg1	properties					975:984	the best mechanical, thermal and UV barrier properties	931:984	the best mechanical, thermal and UV barrier properties	931:984	PLA/RAMCC exhibited the best mechanical, thermal and UV barrier properties.
29920370	0	58	theme	film	59:62	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	0	58	theme	film	59:62	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	5	59	theme	with	647:650	arg1	loading					658:664	with 2 wt% loading	647:664	with 2 wt% loading	647:664	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	5	59	theme	with	647:650	arg1	biocomposite					633:644	PLA/RAMCC biocomposite	623:644	PLA/RAMCC biocomposite (with 2 wt% loading)	623:665	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	7	60	theme	thermal	952:958	arg1	properties					975:984	the best mechanical, thermal and UV barrier properties	931:984	the best mechanical, thermal and UV barrier properties	931:984	PLA/RAMCC exhibited the best mechanical, thermal and UV barrier properties.
29920370	8	61	theme	fractured	991:999	arg1	surfaces					1001:1008	The fractured surfaces	987:1008	The fractured surfaces of the composites	987:1026	The fractured surfaces of the composites showed an even distribution of RAMCC throughout PLA matrix.
29920370	6	62	theme	PLA	879:881	arg1	film					883:886	PLA film	879:886	PLA film	879:886	X-ray Diffraction (XRD) analysis result showed an increase in crystallinity of PLA/RAMCC and reduction in water vapour permeability as compared to PLA film and PLA/MCC composite.
29920370	5	63	theme	2 wt	652:655	arg1	loading					658:664	with 2 wt% loading	647:664	with 2 wt% loading	647:664	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	5	63	theme	2 wt	652:655	arg1	biocomposite					633:644	PLA/RAMCC biocomposite	623:644	PLA/RAMCC biocomposite (with 2 wt% loading)	623:665	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	8	64	theme	PLA	1076:1078	arg1	matrix					1080:1085	PLA matrix	1076:1085	PLA matrix	1076:1085	The fractured surfaces of the composites showed an even distribution of RAMCC throughout PLA matrix.
29920370	10	65	theme	MTT	1282:1284	arg1	method					1286:1291	the MTT method	1278:1291	the MTT method	1278:1291	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	10	66	theme	films	1204:1208	arg1	cytotoxicity					1164:1175	The cytotoxicity	1160:1175	The cytotoxicity of the developed PLA-based films	1160:1208	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	5	67	theme	%	656:656	arg1	loading					658:664	with 2 wt% loading	647:664	with 2 wt% loading	647:664	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	5	67	theme	%	656:656	arg1	biocomposite					633:644	PLA/RAMCC biocomposite	623:644	PLA/RAMCC biocomposite (with 2 wt% loading)	623:665	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	7	68	theme	UV	964:965	arg1	properties					975:984	the best mechanical, thermal and UV barrier properties	931:984	the best mechanical, thermal and UV barrier properties	931:984	PLA/RAMCC exhibited the best mechanical, thermal and UV barrier properties.
29920370	7	69	theme	mechanical	940:949	arg1	properties					975:984	the best mechanical, thermal and UV barrier properties	931:984	the best mechanical, thermal and UV barrier properties	931:984	PLA/RAMCC exhibited the best mechanical, thermal and UV barrier properties.
29920370	2	70	theme	reduced	288:294	arg1	polarity					296:303	reduced polarity	288:303	reduced polarity	288:303	The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC.
29920370	10	71	theme	PLA-based	1194:1202	arg1	films					1204:1208	the developed PLA-based films	1180:1208	the developed PLA-based films	1180:1208	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	1	72	theme	rice	222:225	arg1	RBO					237:239	RBO	237:239	RBO	237:239	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	1	72	theme	rice	222:225	arg1	oil					232:234	rice bran oil	222:234	rice bran oil (RBO)	222:240	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	5	73	theme	PLA	710:712	arg1	film					714:717	PLA film	710:717	PLA film	710:717	PLA/RAMCC biocomposite (with 2 wt% loading) exhibited lower water sorption compared to PLA film and PLA/MCC.
29920370	4	74	theme	based	589:593	arg1	biocomposite					595:606	the PLA based biocomposite	581:606	the PLA based biocomposite	581:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	10	75	theme	developed	1184:1192	arg1	films					1204:1208	the developed PLA-based films	1180:1208	the developed PLA-based films	1180:1208	The cytotoxicity of the developed PLA-based films was evaluated on human dermal fibroblast (HDF) monolayer culture by the MTT method and it has shown that the films were non-cytotoxic thus indicating their biocompatibility and non-toxicity.
29920370	1	76	theme	bran	227:230	arg1	RBO					237:239	RBO	237:239	RBO	237:239	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	1	76	theme	bran	227:230	arg1	oil					232:234	rice bran oil	222:234	rice bran oil (RBO)	222:240	Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO).
29920370	4	77	from	influence	507:515	arg1	properties					567:576	morphological, mechanical, thermal and barrier properties	520:576	morphological, mechanical, thermal and barrier properties of the PLA based biocomposite	520:606	RAMCC and MCC were incorporated into PLA matrix and their influence on morphological, mechanical, thermal and barrier properties of the PLA based biocomposite were analyzed.
29920370	8	78	theme	even	1038:1041	arg1	distribution					1043:1054	an even distribution	1035:1054	an even distribution of RAMCC	1035:1063	The fractured surfaces of the composites showed an even distribution of RAMCC throughout PLA matrix.
29920370	0	79	from	cellulose	117:125	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29920370	0	79	from	cellulose	117:125	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of biocomposite packaging film from poly(lactic acid) and acylated microcrystalline cellulose using rice bran oil.
29305777	4	0	theme	acid	481:484	arg1	%					566:566	96.2%	562:566	96.2%	562:566	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	0	theme	acid	481:484	arg1	percentage					456:465	The percentage	452:465	The percentage	452:465	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	1	theme	sulfate	529:535	arg1	%					566:566	96.2%	562:566	96.2%	562:566	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	1	theme	sulfate	529:535	arg1	percentage					456:465	The percentage	452:465	The percentage	452:465	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	8	2	theme	755 nM	1036:1041	arg1	affinity					1018:1025	a binding affinity	1008:1025	a binding affinity (K D) of 755 nM	1008:1041	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	2	theme	755 nM	1036:1041	arg1	D					1030:1030	K D	1028:1030	K D	1028:1030	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	5	3	theme	CS	630:631	arg1	composition					615:625	The disaccharide composition	598:625	The disaccharide composition of CS	598:631	The disaccharide composition of CS consisted of 4S (49%), 0S (38%) 6S (12%), 2S6S (1.5%) and 2S4S (0.3%).
29305777	9	4	theme	vitreous	1165:1172	arg1	HS					1174:1175	vitreous HS	1165:1175	vitreous HS showing a higher binding affinity	1165:1209	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	2	5	theme	eye	249:251	arg1	vitreous					253:260	bovine eye vitreous	242:260	bovine eye vitreous	242:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	7	6	theme	molecular	852:860	arg1	weights					862:868	The average molecular weights	840:868	The average molecular weights of CS and HS	840:881	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	7	6	theme	molecular	852:860	arg1	148 kDa					888:894	148 kDa	888:894	148 kDa	888:894	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	2	7	theme	bovine	242:247	arg1	vitreous					253:260	bovine eye vitreous	242:260	bovine eye vitreous	242:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	1	8	theme	gel	158:160	arg1	state					162:166	the gel state	154:166	the gel state of eye vitreous humour	154:189	Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour.
29305777	0	9	from	humour	44:49	arg1	Glycosaminoglycans					0:17	Glycosaminoglycans	0:17	Glycosaminoglycans from bovine eye vitreous humour	0:49	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	0	9	from	humour	44:49	arg1	interaction					55:65	interaction	55:65	interaction with collagen type II	55:87	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	8	10	theme	other	1062:1066	arg1	CSB					1084:1086	CSB	1084:1086	CSB	1084:1086	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	10	theme	other	1062:1066	arg1	CSE					1092:1094	CSE	1092:1094	CSE	1092:1094	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	10	theme	other	1062:1066	arg1	GAGs					1068:1071	other GAGs	1062:1071	other GAGs	1062:1071	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	2	11	theme	disaccharide	288:299	arg1	analysis					301:308	disaccharide analysis	288:308	disaccharide analysis	288:308	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	6	12	theme	HS	736:737	arg1	composition					721:731	The disaccharide composition	704:731	The disaccharide composition of HS	704:737	The disaccharide composition of HS consisted of 0S (80%), NS2S (7%), NS (7%), 6S (4%), NS6S (2%), and TriS, 2S and 4S6S (each at 0.1%).
29305777	3	13	theme	collagen	383:390	arg1	type					392:395	collagen type II	383:398	collagen type II	383:398	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	9	14	theme	higher	1187:1192	arg1	affinity					1202:1209	a higher binding affinity	1185:1209	a higher binding affinity	1185:1209	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	1	15	theme	eye	171:173	arg1	humour					184:189	eye vitreous humour	171:189	eye vitreous humour	171:189	Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour.
29305777	0	16	theme	eye	31:33	arg1	humour					44:49	bovine eye vitreous humour	24:49	bovine eye vitreous humour	24:49	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	8	17	with	heparin	966:972	arg1	affinity					1018:1025	a binding affinity	1008:1025	a binding affinity (K D) of 755 nM	1008:1041	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	17	with	heparin	966:972	arg1	D					1030:1030	K D	1028:1030	K D	1028:1030	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	9	18	theme	binding	1194:1200	arg1	affinity					1202:1209	a higher binding affinity	1185:1209	a higher binding affinity	1185:1209	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	1	19	theme	vitreous	175:182	arg1	humour					184:189	eye vitreous humour	171:189	eye vitreous humour	171:189	Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour.
29305777	0	20	theme	bovine	24:29	arg1	humour					44:49	bovine eye vitreous humour	24:49	bovine eye vitreous humour	24:49	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	4	21	theme	heparan	521:527	arg1	HS					538:539	HS	538:539	HS	538:539	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	21	theme	heparan	521:527	arg1	sulfate					529:535	heparan sulfate	521:535	heparan sulfate (HS)	521:540	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	9	22	theme	bovine	1102:1107	arg1	CS					1118:1119	bovine vitreous CS	1102:1119	bovine vitreous CS	1102:1119	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	2	23	from	present	231:237	arg1	vitreous					253:260	bovine eye vitreous	242:260	bovine eye vitreous	242:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	1	24	theme	humour	184:189	arg1	state					162:166	the gel state	154:166	the gel state of eye vitreous humour	154:189	Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour.
29305777	5	25	theme	disaccharide	602:613	arg1	composition					615:625	The disaccharide composition	598:625	The disaccharide composition of CS	598:631	The disaccharide composition of CS consisted of 4S (49%), 0S (38%) 6S (12%), 2S6S (1.5%) and 2S4S (0.3%).
29305777	9	26	theme	vitreous	1109:1116	arg1	CS					1118:1119	bovine vitreous CS	1102:1119	bovine vitreous CS	1102:1119	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	3	27	theme	surface	419:425	arg1	SPR					446:448	SPR	446:448	SPR	446:448	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	3	27	theme	surface	419:425	arg1	resonance					435:443	surface plasmon resonance	419:443	surface plasmon resonance (SPR)	419:449	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	7	28	theme	CS	873:874	arg1	weights					862:868	The average molecular weights	840:868	The average molecular weights of CS and HS	840:881	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	7	28	theme	CS	873:874	arg1	148 kDa					888:894	148 kDa	888:894	148 kDa	888:894	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	0	29	theme	vitreous	35:42	arg1	humour					44:49	bovine eye vitreous humour	24:49	bovine eye vitreous humour	24:49	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	4	30	theme	chondroitin	492:502	arg1	sulfate					504:510	chondroitin sulfate	492:510	chondroitin sulfate (CS)	492:515	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	30	theme	chondroitin	492:502	arg1	CS					513:514	CS	513:514	CS	513:514	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	8	31	theme	binding	1010:1016	arg1	affinity					1018:1025	a binding affinity	1008:1025	a binding affinity (K D) of 755 nM	1008:1041	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	31	theme	binding	1010:1016	arg1	D					1030:1030	K D	1028:1030	K D	1028:1030	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	6	32	theme	disaccharide	708:719	arg1	composition					721:731	The disaccharide composition	704:731	The disaccharide composition of HS	704:737	The disaccharide composition of HS consisted of 0S (80%), NS2S (7%), NS (7%), 6S (4%), NS6S (2%), and TriS, 2S and 4S6S (each at 0.1%).
29305777	8	33	theme	collagen	940:947	arg1	type					949:952	collagen type II	940:955	collagen type II	940:955	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	3	34	theme	plasmon	427:433	arg1	SPR					446:448	SPR	446:448	SPR	446:448	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	3	34	theme	plasmon	427:433	arg1	resonance					435:443	surface plasmon resonance	419:443	surface plasmon resonance (SPR)	419:449	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	4	35	theme	hyaluronic	470:479	arg1	HA					487:488	HA	487:488	HA	487:488	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	35	theme	hyaluronic	470:479	arg1	acid					481:484	hyaluronic acid	470:484	hyaluronic acid (HA)	470:489	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	2	36	theme	chromatography-mass	320:338	arg1	spectrometry					340:351	liquid chromatography-mass spectrometry	313:351	liquid chromatography-mass spectrometry	313:351	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	3	37	with	interaction	358:368	arg1	type					392:395	collagen type II	383:398	collagen type II	383:398	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	2	38	theme	liquid	313:318	arg1	spectrometry					340:351	liquid chromatography-mass spectrometry	313:351	liquid chromatography-mass spectrometry	313:351	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	4	39	theme	sulfate	504:510	arg1	%					566:566	96.2%	562:566	96.2%	562:566	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	39	theme	sulfate	504:510	arg1	percentage					456:465	The percentage	452:465	The percentage	452:465	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	2	40	theme	present	231:237	arg1	GAGs					226:229	GAGs	226:229	GAGs present in bovine eye vitreous	226:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	0	41	with	Glycosaminoglycans	0:17	arg1	type					81:84	collagen type II	72:87	collagen type II	72:87	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	7	42	theme	average	844:850	arg1	weights					862:868	The average molecular weights	840:868	The average molecular weights of CS and HS	840:881	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	7	42	theme	average	844:850	arg1	148 kDa					888:894	148 kDa	888:894	148 kDa	888:894	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	2	43	theme	GAGs	226:229	arg1	composition					211:221	the composition	207:221	the composition of GAGs present in bovine eye vitreous	207:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	0	44	with	interaction	55:65	arg1	type					81:84	collagen type II	72:87	collagen type II	72:87	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	1	45	theme	important	124:132	arg1	role					134:137	an important role	121:137	an important role	121:137	Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour.
29305777	2	46	from	vitreous	253:260	arg1	present					231:237	present	231:237	present	231:237	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	2	47	attach	present	231:237	arg1	vitreous					253:260	bovine eye vitreous	242:260	bovine eye vitreous	242:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	2	47	attach	present	231:237	arg2	GAGs					226:229	GAGs	226:229	GAGs present in bovine eye vitreous	226:260	In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry.
29305777	0	48	theme	collagen	72:79	arg1	type					81:84	collagen type II	72:87	collagen type II	72:87	Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.
29305777	8	49	theme	K	1028:1028	arg1	affinity					1018:1025	a binding affinity	1008:1025	a binding affinity (K D) of 755 nM	1008:1041	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	8	49	theme	K	1028:1028	arg1	D					1030:1030	K D	1028:1030	K D	1028:1030	SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE.
29305777	9	50	theme	collagen	1142:1149	arg1	type					1151:1154	collagen type II	1142:1157	collagen type II	1142:1157	Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
29305777	7	51	theme	HS	880:881	arg1	weights					862:868	The average molecular weights	840:868	The average molecular weights of CS and HS	840:881	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	7	51	theme	HS	880:881	arg1	148 kDa					888:894	148 kDa	888:894	148 kDa	888:894	The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively.
29305777	4	52	theme	GAG	552:554	arg1	%					566:566	96.2%	562:566	96.2%	562:566	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	4	52	theme	GAG	552:554	arg1	percentage					456:465	The percentage	452:465	The percentage	452:465	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
29305777	3	53	theme	GAGs	373:376	arg1	interaction					358:368	The interaction	354:368	The interaction of GAGs with collagen type II	354:398	The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR).
29305777	4	54	theme	total	546:550	arg1	GAG					552:554	total GAG	546:554	total GAG	546:554	The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively.
31295575	2	0	theme	co-culturing	371:382	arg1	ATCC					411:414	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	2	1	from	nanocomposites	304:317	arg1	form					335:338	the pellicle form	322:338	the pellicle form	322:338	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	4	2	theme	dynamic	610:616	arg1	growth					618:623	The dynamic growth	606:623	The dynamic growth of the strains	606:638	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	0	3	theme	Lactococcus	114:124	arg1	lactis					126:131	Gluconacetobacter hansenii and Lactococcus lactis	83:131	lactis	126:131	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.
31295575	1	4	theme	based	194:198	arg1	composites					200:209	Bacterial cellulose (BC) based composites	169:209	Bacterial cellulose (BC) based composites	169:209	Bacterial cellulose (BC) based composites have been widely studied in the biomedical field.
31295575	5	5	theme	larger	942:947	arg1	ribbons					949:955	20-40 nm and larger ribbons	929:955	20-40 nm and larger ribbons between 180 and 360 nm	929:978	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	3	6	theme	constant	575:582	arg1	rate					589:592	the constant feed rate	571:592	the constant feed rate of glucose	571:603	The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose.
31295575	3	7	theme	glucose	597:603	arg1	rate					589:592	the constant feed rate	571:592	the constant feed rate of glucose	571:603	The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose.
31295575	6	8	from	break	1018:1022	arg1	capacity					1046:1053	the water holding capacity	1028:1053	the water holding capacity of BC/HA	1028:1062	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	6	8	from	break	1018:1022	arg1	strain					1008:1013	The strain	1004:1013	The strain at break	1004:1022	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	3	9	theme	feed	584:587	arg1	rate					589:592	the constant feed rate	571:592	the constant feed rate of glucose	571:603	The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose.
31295575	4	10	theme	48 h.	735:739	arg1	analysis					745:752	48 h. XRD analysis	735:752	48 h. XRD analysis	735:752	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	0	11	theme	circulating	149:159	arg1	system					161:166	a two-vessel circulating system	136:166	a two-vessel circulating system	136:166	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.
31295575	6	12	theme	holding	1038:1044	arg1	capacity					1046:1053	the water holding capacity	1028:1053	the water holding capacity of BC/HA	1028:1062	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	2	13	theme	hyaluronic	287:296	arg1	acid					298:301	hyaluronic acid	287:301	hyaluronic acid	287:301	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	2	13	theme	hyaluronic	287:296	arg1	BC/HA					280:284	the BC/HA	276:284	the BC/HA (hyaluronic acid) nanocomposites in the pellicle form	276:338	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	5	14	theme	20-40 nm	929:936	arg1	ribbons					949:955	20-40 nm and larger ribbons	929:955	20-40 nm and larger ribbons between 180 and 360 nm	929:978	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	2	15	theme	Lactococcus	426:436	arg1	lactis					438:443	Lactococcus lactis APJ3	426:448	Lactococcus lactis APJ3 in a novel two-vessel circulating system	426:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	0	16	theme	two-vessel	138:147	arg1	system					161:166	a two-vessel circulating system	136:166	a two-vessel circulating system	136:166	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.
31295575	4	17	theme	crystalline	848:858	arg1	sizes					860:864	the crystalline sizes	844:864	the crystalline sizes	844:864	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	0	18	theme	Bacterial	0:8	arg1	acid					31:34	Bacterial cellulose/hyaluronic acid	0:34	Bacterial cellulose/hyaluronic acid	0:34	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.
31295575	0	19	theme	cellulose/hyaluronic	10:29	arg1	acid					31:34	Bacterial cellulose/hyaluronic acid	0:34	Bacterial cellulose/hyaluronic acid	0:34	Bacterial cellulose/hyaluronic acid nanocomposites production through co-culturing Gluconacetobacter hansenii and Lactococcus lactis in a two-vessel circulating system.
31295575	2	20	theme	pellicle	326:333	arg1	form					335:338	the pellicle form	322:338	the pellicle form	322:338	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	6	21	theme	BC/HA	1058:1062	arg1	capacity					1046:1053	the water holding capacity	1028:1053	the water holding capacity of BC/HA	1028:1062	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	6	21	theme	BC/HA	1058:1062	arg1	strain					1008:1013	The strain	1004:1013	The strain at break	1004:1022	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	2	22	theme	circulating	472:482	arg1	system					484:489	a novel two-vessel circulating system	453:489	a novel two-vessel circulating system	453:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	4	23	dep	indicated	754:762	arg1	affect					793:798	affect	793:798	affect the crystallinity of cellulose	793:829	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	4	23	dep	indicated	754:762	arg1	increase					835:842	increase	835:842	increase the crystalline sizes	835:864	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	5	24	theme	ribbons	949:955	arg1	width					920:924	the width	916:924	the width of 20-40 nm and larger ribbons between 180 and 360 nm	916:978	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	4	25	theme	HA	780:781	arg1	presence					768:775	the presence	764:775	the presence of HA	764:781	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	2	26	theme	two-vessel	461:470	arg1	system					484:489	a novel two-vessel circulating system	453:489	a novel two-vessel circulating system	453:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	2	27	theme	novel	455:459	arg1	system					484:489	a novel two-vessel circulating system	453:489	a novel two-vessel circulating system	453:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	5	28	located	observed	985:992	arg1	BC/HA					997:1001	BC/HA	997:1001	BC/HA	997:1001	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	5	28	located	observed	985:992	arg2	ribbons					901:907	more ribbons	896:907	more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm	896:978	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	5	29	theme	more	896:899	arg1	ribbons					901:907	more ribbons	896:907	more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm	896:978	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	6	30	theme	water	1032:1036	arg1	capacity					1046:1053	the water holding capacity	1028:1053	the water holding capacity of BC/HA	1028:1062	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	5	31	theme	FESEM	871:875	arg1	images					877:882	The FESEM images	867:882	The FESEM images	867:882	The FESEM images showed that more ribbons within the width of 20-40 nm and larger ribbons between 180 and 360 nm were observed in BC/HA.
31295575	4	32	theme	cellulose	821:829	arg1	crystallinity					804:816	the crystallinity	800:816	the crystallinity of cellulose	800:829	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	1	33	theme	biomedical	243:252	arg1	field					254:258	the biomedical field	239:258	the biomedical field	239:258	Bacterial cellulose (BC) based composites have been widely studied in the biomedical field.
31295575	2	34	from	ATCC	411:414	arg1	system					484:489	a novel two-vessel circulating system	453:489	a novel two-vessel circulating system	453:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	3	35	theme	L.	528:529	arg1	lactis					531:536	L. lactis	528:536	L. lactis	528:536	The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose.
31295575	2	36	theme	BC/HA	280:284	arg1	nanocomposites					304:317	the BC/HA (hyaluronic acid) nanocomposites	276:317	the BC/HA (hyaluronic acid) nanocomposites in the pellicle form	276:338	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	1	37	theme	Bacterial	169:177	arg1	BC					190:191	BC	190:191	BC	190:191	Bacterial cellulose (BC) based composites have been widely studied in the biomedical field.
31295575	1	37	theme	Bacterial	169:177	arg1	cellulose					179:187	Bacterial cellulose	169:187	Bacterial cellulose (BC) based composites	169:209	Bacterial cellulose (BC) based composites have been widely studied in the biomedical field.
31295575	4	38	theme	strains	632:638	arg1	growth					618:623	The dynamic growth	606:623	The dynamic growth of the strains	606:638	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	4	39	theme	XRD	741:743	arg1	analysis					745:752	48 h. XRD analysis	735:752	48 h. XRD analysis	735:752	The dynamic growth of the strains revealed that L. lactis was mainly growing within 48 h while G. hansenii started to grow after 48 h. XRD analysis indicated the presence of HA would not affect the crystallinity of cellulose but increase the crystalline sizes.
31295575	1	40	theme	cellulose	179:187	arg1	composites					200:209	Bacterial cellulose (BC) based composites	169:209	Bacterial cellulose (BC) based composites	169:209	Bacterial cellulose (BC) based composites have been widely studied in the biomedical field.
31295575	3	41	theme	HA	513:514	arg1	concentration					496:508	The concentration	492:508	The concentration of HA secreted by L. lactis	492:536	The concentration of HA secreted by L. lactis was controlled through adjusting the constant feed rate of glucose.
31295575	2	42	from	lactis	438:443	arg1	system					484:489	a novel two-vessel circulating system	453:489	a novel two-vessel circulating system	453:489	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	2	43	theme	hansenii	402:409	arg1	ATCC					411:414	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31295575	6	44	theme	HA	1100:1101	arg1	concentration					1083:1095	the concentration	1079:1095	the concentration of HA	1079:1101	The strain at break and the water holding capacity of BC/HA increased with the concentration of HA.
31295575	2	45	theme	Gluconacetobacter	384:400	arg1	ATCC					411:414	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	co-culturing Gluconacetobacter hansenii ATCC 23769	371:420	In this study, the BC/HA (hyaluronic acid) nanocomposites in the pellicle form were directly produced through co-culturing Gluconacetobacter hansenii ATCC 23769 and Lactococcus lactis APJ3 in a novel two-vessel circulating system.
31755646	5	0	theme	combined	1154:1161	arg1	ions					1177:1180	combined Ca2+ and Fe3+ ions	1154:1180	combined Ca2+ and Fe3+ ions	1154:1180	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	3	1	theme	Boston	512:517	arg1	disk					523:526	the Boston ivy disk	508:526	the Boston ivy disk	508:526	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	5	2	with	ligandation	1137:1147	arg1	ions					1177:1180	combined Ca2+ and Fe3+ ions	1154:1180	combined Ca2+ and Fe3+ ions	1154:1180	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	5	3	theme	pressure-mediated	1214:1230	arg1	technology					1241:1250	a pressure-mediated adhesion technology	1212:1250	a pressure-mediated adhesion technology	1212:1250	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	5	4	theme	Ca2+	1163:1166	arg1	ions					1177:1180	combined Ca2+ and Fe3+ ions	1154:1180	combined Ca2+ and Fe3+ ions	1154:1180	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	4	5	theme	nanoparticle	772:783	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	5	6	theme	breaks	1016:1021	arg1	pressurization					988:1001	the pressurization	984:1001	the pressurization of the patch breaks the microgels	984:1035	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	5	7	theme	adhesion	1232:1239	arg1	technology					1241:1250	a pressure-mediated adhesion technology	1212:1250	a pressure-mediated adhesion technology	1212:1250	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	4	8	theme	bolaamphiphile	757:770	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	2	9	theme	practical	322:330	arg1	applicability					332:344	their practical applicability	316:344	their practical applicability as an adhesive	316:359	While the cellular structures and adhesive substances involved have been identified for decades, their practical applicability as an adhesive has not yet been demonstrated.
31755646	5	10	theme	DOPA-C7	1121:1127	arg1	NPs					1129:1131	DOPA-C7 NPs	1121:1131	DOPA-C7 NPs	1121:1131	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	3	11	theme	compositional	482:494	arg1	features					496:503	structural and compositional features	467:503	structural and compositional features of the Boston ivy disk	467:526	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	4	12	theme	DOPA-C7	786:792	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	13	theme	porous	639:644	arg1	microchannels					646:658	porous microchannels	639:658	porous microchannels	639:658	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	5	14	theme	technology	1241:1250	arg1	development					1197:1207	development	1197:1207	development of a pressure-mediated adhesion technology	1197:1250	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	3	15	theme	disk-inspired	405:417	arg1	system					439:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	4	16	used	occupied	664:671	arg2	microchannels					646:658	porous microchannels	639:658	porous microchannels	639:658	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	1	17	theme	sticky	167:172	arg1	substance					174:182	a sticky substance to perform binding and attachment	165:216	a sticky substance to perform binding and attachment	165:216	Boston ivy (Parthenocissus tricuspidata) climbs brick walls using its tendril disks, which excrete a sticky substance to perform binding and attachment.
31755646	4	18	theme	ivy	626:628	arg1	disk					609:612	the sticky disk	598:612	the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site	598:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	3	19	theme	thin	556:559	arg1	patches					575:581	thin adhesive film patches	556:581	thin adhesive film patches	556:581	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	3	20	theme	film	428:431	arg1	system					439:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	4	21	theme	cylindrical	866:876	arg1	holes					878:882	the cylindrical holes	862:882	the cylindrical holes that are periodically micropatterned on the flexible stencil film	862:948	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	3	22	theme	adhesive	561:568	arg1	patches					575:581	thin adhesive film patches	556:581	thin adhesive film patches	556:581	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	3	23	theme	patch	433:437	arg1	system					439:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	0	24	theme	Pressure-Mediated	25:41	arg1	Patches					57:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Boston Ivy Disk-Inspired Pressure-Mediated Adhesive Film Patches.
31755646	4	25	theme	bound	717:721	arg1	site					723:726	the bound site	713:726	the bound site	713:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	26	dep	disk	609:612	arg1	analogy					587:593	analogy	587:593	analogy	587:593	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	27	theme	3,4-dihydroxylphenylalanine	729:755	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	5	28	with	crosslinking	1103:1114	arg1	NPs					1129:1131	DOPA-C7 NPs	1121:1131	DOPA-C7 NPs	1121:1131	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	0	29	theme	Disk-Inspired	11:23	arg1	Patches					57:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Boston Ivy Disk-Inspired Pressure-Mediated Adhesive Film Patches.
31755646	1	30	theme	brick	114:118	arg1	walls					120:124	brick walls	114:124	brick walls	114:124	Boston ivy (Parthenocissus tricuspidata) climbs brick walls using its tendril disks, which excrete a sticky substance to perform binding and attachment.
31755646	3	31	theme	structural	467:476	arg1	features					496:503	structural and compositional features	467:503	structural and compositional features of the Boston ivy disk	467:526	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	0	32	theme	Film	52:55	arg1	Patches					57:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Boston Ivy Disk-Inspired Pressure-Mediated Adhesive Film Patches.
31755646	2	33	theme	cellular	229:236	arg1	structures					238:247	cellular structures	229:247	cellular structures	229:247	While the cellular structures and adhesive substances involved have been identified for decades, their practical applicability as an adhesive has not yet been demonstrated.
31755646	4	34	theme	mature	619:624	arg1	ivy					626:628	a mature ivy	617:628	a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site	617:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	0	35	theme	Adhesive	43:50	arg1	Patches					57:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Disk-Inspired Pressure-Mediated Adhesive Film Patches	11:63	Boston Ivy Disk-Inspired Pressure-Mediated Adhesive Film Patches.
31755646	3	36	theme	adhesive	419:426	arg1	system					439:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	0	37	dep	Patches	57:63	arg1	Ivy					7:9	Ivy	7:9	Ivy	7:9	Boston Ivy Disk-Inspired Pressure-Mediated Adhesive Film Patches.
31755646	5	38	dep	breaks	1016:1021	arg1	microgels					1027:1035	the microgels	1023:1035	the patch breaks the microgels	1006:1035	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	5	39	theme	Fe3+	1172:1175	arg1	ions					1177:1180	combined Ca2+ and Fe3+ ions	1154:1180	combined Ca2+ and Fe3+ ions	1154:1180	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	3	40	theme	film	570:573	arg1	patches					575:581	thin adhesive film patches	556:581	thin adhesive film patches	556:581	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	3	41	theme	ivy	401:403	arg1	system					439:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system	392:444	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	3	42	theme	patches	575:581	arg1	form					548:551	a form	546:551	a form of thin adhesive film patches	546:581	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	4	43	theme	catechol-containing	676:694	arg1	microgranules					696:708	catechol-containing microgranules	676:708	catechol-containing microgranules on the bound site	676:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	44	theme	stencil	937:943	arg1	film					945:948	the flexible stencil film	924:948	the flexible stencil film	924:948	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	5	45	theme	patch	1010:1014	arg1	breaks					1016:1021	the patch breaks	1006:1021	the patch breaks the microgels	1006:1035	Finally, it is demonstrated that the pressurization of the patch breaks the microgels filled in the holes, releasing the polysaccharides and leading to crosslinking with DOPA-C7 NPs via ligandation with combined Ca2+ and Fe3+ ions, thus enabling development of a pressure-mediated adhesion technology.
31755646	4	46	theme	flexible	928:935	arg1	film					945:948	the flexible stencil film	924:948	the flexible stencil film	924:948	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	2	47	theme	adhesive	253:260	arg1	substances					262:271	adhesive substances	253:271	adhesive substances	253:271	While the cellular structures and adhesive substances involved have been identified for decades, their practical applicability as an adhesive has not yet been demonstrated.
31755646	1	48	theme	tendril	136:142	arg1	disks					144:148	its tendril disks	132:148	its tendril disks	132:148	Boston ivy (Parthenocissus tricuspidata) climbs brick walls using its tendril disks, which excrete a sticky substance to perform binding and attachment.
31755646	4	49	from	microgranules	696:708	arg1	site					723:726	the bound site	713:726	the bound site	713:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	50	theme	alginate	805:812	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	3	51	theme	ivy	519:521	arg1	disk					523:526	the Boston ivy disk	508:526	the Boston ivy disk	508:526	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
31755646	2	52	dep	structures	238:247	arg1	the					225:227	the	225:227	the	225:227	While the cellular structures and adhesive substances involved have been identified for decades, their practical applicability as an adhesive has not yet been demonstrated.
31755646	4	53	theme	sticky	602:607	arg1	disk					609:612	the sticky disk	598:612	the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site	598:726	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	4	54	theme	-coated	797:803	arg1	microgels					814:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels	729:822	In analogy to the sticky disk of a mature ivy in which porous microchannels are occupied by catechol-containing microgranules on the bound site, 3,4-dihydroxylphenylalanine bolaamphiphile nanoparticle (DOPA-C7 NP)-coated alginate microgels are two-dimensionally positioned into the cylindrical holes that are periodically micropatterned on the flexible stencil film.
31755646	3	55	theme	disk	523:526	arg1	features					496:503	structural and compositional features	467:503	structural and compositional features of the Boston ivy disk	467:526	A Boston ivy disk-inspired adhesive film patch system is reported in which structural and compositional features of the Boston ivy disk are mimicked with a form of thin adhesive film patches.
29345945	10	0	theme	slowest	1923:1929	arg1	process					1942:1948	The slowest adsorption process	1919:1948	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure	1919:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	11	1	theme	>	2182:2182	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	1	theme	>	2182:2182	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	2	2	theme	physical	307:314	arg1	adsorption					316:325	physical adsorption	307:325	physical adsorption	307:325	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	7	3	theme	sorption	1211:1218	arg1	capacity					1220:1227	a higher sorption capacity	1202:1227	a higher sorption capacity	1202:1227	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	4	theme	acid	1263:1266	arg1	ChS2					1287:1290	ChS2	1287:1290	ChS2	1287:1290	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	4	theme	acid	1263:1266	arg1	dye					1282:1284	the acid red 88 (AR88) dye	1259:1284	the acid red 88 (AR88) dye (ChS2)	1259:1291	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	11	5	theme	AO7	2177:2179	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	5	theme	AO7	2177:2179	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	3	6	theme	polymer	488:494	arg1	distribution					472:483	a homogeneous distribution	458:483	a homogeneous distribution of polymer	458:494	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	8	7	dep	applied	1570:1576	arg1	first-order					1579:1589	first-order	1579:1589	first-order	1579:1589	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	MOE					1664:1666	fractal-like MOE	1651:1666	fractal-like MOE	1651:1666	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	second-order					1592:1603	second-order	1592:1603	second-order	1592:1603	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	multiexponential					1629:1644	multiexponential	1629:1644	multiexponential	1629:1644	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	intraparticle					1679:1691	intraparticle	1679:1691	intraparticle	1679:1691	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	1,2-order					1612:1620	mixed 1,2-order	1606:1620	mixed 1,2-order (MOE)	1606:1626	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	7	dep	applied	1570:1576	arg1	MOE					1623:1625	MOE	1623:1625	MOE	1623:1625	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	5	8	with	correlation	871:881	arg1	structure					902:910	the composite structure	888:910	the composite structure	888:910	The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished.
29345945	8	9	theme	mixed	1606:1610	arg1	1,2-order					1612:1620	mixed 1,2-order	1606:1620	mixed 1,2-order (MOE)	1606:1626	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	9	theme	mixed	1606:1610	arg1	MOE					1623:1625	MOE	1623:1625	MOE	1623:1625	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	6	10	theme	Langmuir	1021:1028	arg1	equations					1030:1038	Langmuir equations	1021:1038	Langmuir equations	1021:1038	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	8	11	from	behavior	1477:1484	arg1	processes					1528:1536	the adsorption processes	1513:1536	the adsorption processes	1513:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	4	12	with	material	672:679	arg1	surface					752:758	a more negatively charged surface	726:758	a more negatively charged surface (pHPZC = 4.2)	726:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	12	with	material	672:679	arg1	area					705:708	a specific surface area	686:708	a specific surface area of 600 m2/g	686:720	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	12	with	material	672:679	arg1	pHPZC					761:765	pHPZC = 4.2	761:771	pHPZC = 4.2	761:771	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	3	13	from	composites	436:445	arg1	pores					528:532	relatively wide pores	512:532	relatively wide pores	512:532	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	3	13	from	composites	436:445	arg1	surface					503:509	the surface	499:509	the surface	499:509	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	3	13	from	composites	436:445	arg1	area					556:559	specific surface area	539:559	specific surface area	539:559	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	2	14	theme	fumed	284:288	arg1	ChS3					298:301	ChS3	298:301	ChS3	298:301	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	2	14	theme	fumed	284:288	arg1	silica					290:295	fumed silica	284:295	fumed silica (ChS3)	284:302	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	8	15	theme	deeper	1449:1454	arg1	understanding					1456:1468	a deeper understanding	1447:1468	a deeper understanding of the behavior of immobilized chitosan in the adsorption processes	1447:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	7	16	theme	sol-gel	1391:1397	arg1	[ChS1					1406:1410	the sol-gel method [ChS1	1387:1410	the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye]	1387:1440	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	17	from	chitosan	1153:1160	arg1	surface					1190:1196	a silica surface	1181:1196	a silica surface	1181:1196	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	1	18	theme	hybrid	187:192	arg1	materials					194:202	final hybrid materials	181:202	final hybrid materials	181:202	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	4	19	theme	sol-gel	794:800	arg1	reaction					802:809	the sol-gel reaction	790:809	the sol-gel reaction	790:809	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	11	20	dep	halftimes	2231:2239	arg1	min					2275:2277	10.5 < 15.7 < 23.7 < 34.9 < 42.9 min	2242:2277	10.5 < 15.7 < 23.7 < 34.9 < 42.9 min	2242:2277	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	8	21	theme	behavior	1477:1484	arg1	understanding					1456:1468	a deeper understanding	1447:1468	a deeper understanding of the behavior of immobilized chitosan in the adsorption processes	1447:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	11	22	theme	various	2120:2126	arg1	dyes					2128:2131	various dyes	2120:2131	various dyes	2120:2131	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	6	23	theme	isotherm	1085:1092	arg1	data					1094:1097	adsorption isotherm data	1074:1097	adsorption isotherm data	1074:1097	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	5	24	theme	composite	892:900	arg1	structure					902:910	the composite structure	888:910	the composite structure	888:910	The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished.
29345945	3	25	theme	wide	523:526	arg1	pores					528:532	relatively wide pores	512:532	relatively wide pores	512:532	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	8	26	theme	immobilized	1489:1499	arg1	chitosan					1501:1508	immobilized chitosan	1489:1508	immobilized chitosan in the adsorption processes	1489:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	10	27	theme	molecules	1990:1998	arg1	rate					1978:1981	the diffusion rate	1964:1981	the diffusion rate of dye molecules into the internal space of the pore structure	1964:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	9	28	attach	removed	1845:1851	arg1	solution					1862:1869	the solution	1858:1869	the solution	1858:1869	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	9	28	attach	removed	1845:1851	arg2	%					1828:1828	50%	1826:1828	50% of the dye	1826:1839	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	9	28	attach	removed	1845:1851	arg2	dye					1837:1839	the dye	1833:1839	the dye	1833:1839	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	7	29	dep	[ChS1	1406:1410	arg1	mmol/g					1418:1423	0.05 mmol/g	1413:1423	0.05 mmol/g for the AO8 dye	1413:1439	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	0	30	theme	Dyes	64:67	arg1	Removal					38:44	Removal	38:44	Removal of Sulfonated Azo Dyes from Aqueous Solutions	38:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	3	31	theme	specific	539:546	arg1	area					556:559	specific surface area	539:559	specific surface area	539:559	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	8	32	theme	adsorption	1517:1526	arg1	processes					1528:1536	the adsorption processes	1513:1536	the adsorption processes	1513:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	1	33	theme	chitosan	129:136	arg1	method					153:158	the chitosan immobilization method	125:158	the chitosan immobilization method	125:158	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	0	34	theme	Aqueous	74:80	arg1	Solutions					82:90	Aqueous Solutions	74:90	Aqueous Solutions	74:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	1	35	theme	method	153:158	arg1	influence					112:120	the influence	108:120	the influence of the chitosan immobilization method on the properties of final hybrid materials	108:202	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	4	36	theme	microporous	644:654	arg1	material					672:679	The microporous chitosan-silica material	640:679	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2)	640:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	8	37	theme	pore	1697:1700	arg1	equations					1718:1726	pore diffusion model equations	1697:1726	pore diffusion model equations	1697:1726	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	38	theme	kinetic	1547:1553	arg1	equations					1555:1563	various kinetic equations	1539:1563	various kinetic equations	1539:1563	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	5	39	theme	azo	830:832	arg1	adsorption					838:847	azo dye adsorption	830:847	azo dye adsorption	830:847	The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished.
29345945	7	40	theme	AO8	1332:1334	arg1	ChS1					1342:1345	ChS1	1342:1345	ChS1	1342:1345	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	40	theme	AO8	1332:1334	arg1	dye					1337:1339	the acid orange 8 (AO8) dye	1313:1339	the acid orange 8 (AO8) dye (ChS1)	1313:1346	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	10	41	theme	structure	2036:2044	arg1	space					2018:2022	the internal space	2005:2022	the internal space of the pore structure	2005:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	3	42	theme	physical	376:383	arg1	immobilization					385:398	physical immobilization	376:398	physical immobilization of chitosan	376:410	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	8	43	theme	model	1712:1716	arg1	equations					1718:1726	pore diffusion model equations	1697:1726	pore diffusion model equations	1697:1726	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	1	44	from	influence	112:120	arg1	properties					167:176	the properties	163:176	the properties of final hybrid materials	163:202	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	7	45	dep	chitosan	1153:1160	arg1	ChS2					1172:1175	ChS2	1172:1175	ChS2	1172:1175	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	45	dep	chitosan	1153:1160	arg1	ChS1					1163:1166	ChS1	1163:1166	ChS1	1163:1166	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	6	46	theme	generalized	935:945	arg1	equation					956:963	The generalized Langmuir equation	931:963	The generalized Langmuir equation	931:963	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	6	46	theme	generalized	935:945	arg1	Langmuir-Freundlich					997:1015	Langmuir-Freundlich	997:1015	Langmuir-Freundlich	997:1015	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	0	47	from	Removal	38:44	arg1	Solutions					82:90	Aqueous Solutions	74:90	Aqueous Solutions	74:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	9	48	theme	AO8	1744:1746	arg1	dye					1748:1750	AO8 dye	1744:1750	AO8 dye	1744:1750	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	0	49	theme	Chitosan-Silica	0:14	arg1	Composites					23:32	Chitosan-Silica Hybrid Composites	0:32	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.	0:91	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	9	50	theme	adsorption	1757:1766	arg1	rates					1768:1772	the adsorption rates	1753:1772	the adsorption rates	1753:1772	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	3	51	theme	homogeneous	460:470	arg1	distribution					472:483	a homogeneous distribution	458:483	a homogeneous distribution of polymer	458:494	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	7	52	theme	orange	1322:1327	arg1	ChS1					1342:1345	ChS1	1342:1345	ChS1	1342:1345	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	52	theme	orange	1322:1327	arg1	dye					1337:1339	the acid orange 8 (AO8) dye	1313:1339	the acid orange 8 (AO8) dye (ChS1)	1313:1346	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	53	theme	method	1399:1404	arg1	[ChS1					1406:1410	the sol-gel method [ChS1	1387:1410	the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye]	1387:1440	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	3	54	theme	m2/g	574:577	arg1	pores					528:532	relatively wide pores	512:532	relatively wide pores	512:532	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	3	54	theme	m2/g	574:577	arg1	surface					503:509	the surface	499:509	the surface	499:509	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	3	54	theme	m2/g	574:577	arg1	area					556:559	specific surface area	539:559	specific surface area	539:559	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	4	55	theme	m2/g	717:720	arg1	surface					752:758	a more negatively charged surface	726:758	a more negatively charged surface (pHPZC = 4.2)	726:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	55	theme	m2/g	717:720	arg1	area					705:708	a specific surface area	686:708	a specific surface area of 600 m2/g	686:720	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	55	theme	m2/g	717:720	arg1	pHPZC					761:765	pHPZC = 4.2	761:771	pHPZC = 4.2	761:771	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	0	56	theme	Sulfonated	49:58	arg1	Dyes					64:67	Sulfonated Azo Dyes	49:67	Sulfonated Azo Dyes from Aqueous Solutions	49:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	11	57	theme	acid	2200:2203	arg1	halftimes					2231:2239	halftimes	2231:2239	halftimes	2231:2239	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	57	theme	acid	2200:2203	arg1	AO8					2226:2228	acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8	2164:2228	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	7	58	contain	has	1198:1200	arg2	capacity					1220:1227	a higher sorption capacity	1202:1227	a higher sorption capacity	1202:1227	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	58	contain	has	1198:1200	arg1	chitosan					1153:1160	physically adsorbed chitosan	1133:1160	physically adsorbed chitosan (ChS1 and ChS2) on a silica surface	1133:1196	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	4	59	theme	surface	697:703	arg1	area					705:708	a specific surface area	686:708	a specific surface area of 600 m2/g	686:720	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	11	60	theme	acid	2164:2167	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	60	theme	acid	2164:2167	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	61	dep	order	2157:2161	arg1	halftimes					2231:2239	halftimes	2231:2239	halftimes	2231:2239	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	61	dep	order	2157:2161	arg1	AO8					2226:2228	acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8	2164:2228	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	4	62	theme	charged	744:750	arg1	surface					752:758	a more negatively charged surface	726:758	a more negatively charged surface (pHPZC = 4.2)	726:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	62	theme	charged	744:750	arg1	pHPZC					761:765	pHPZC = 4.2	761:771	pHPZC = 4.2	761:771	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	7	63	theme	adsorption	1104:1113	arg1	study					1115:1119	The adsorption study	1100:1119	The adsorption study	1100:1119	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	11	64	theme	orange	2184:2189	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	64	theme	orange	2184:2189	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	7	65	theme	red	1268:1270	arg1	ChS2					1287:1290	ChS2	1287:1290	ChS2	1287:1290	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	65	theme	red	1268:1270	arg1	dye					1282:1284	the acid red 88 (AR88) dye	1259:1284	the acid red 88 (AR88) dye (ChS2)	1259:1291	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	2	66	theme	mesoporous	262:271	arg1	surface					251:257	the surface	247:257	the surface of mesoporous (ChS2) and fumed silica (ChS3)	247:302	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	3	67	with	composites	436:445	arg1	distribution					472:483	a homogeneous distribution	458:483	a homogeneous distribution of polymer	458:494	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	9	68	theme	dye	1837:1839	arg1	%					1828:1828	50%	1826:1828	50% of the dye	1826:1839	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	9	68	theme	dye	1837:1839	arg1	dye					1837:1839	the dye	1833:1839	the dye	1833:1839	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	2	69	theme	silica	290:295	arg1	surface					251:257	the surface	247:257	the surface of mesoporous (ChS2) and fumed silica (ChS3)	247:302	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	10	70	theme	adsorption	1931:1940	arg1	process					1942:1948	The slowest adsorption process	1919:1948	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure	1919:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	1	71	theme	final	181:185	arg1	materials					194:202	final hybrid materials	181:202	final hybrid materials	181:202	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	0	72	from	Solutions	82:90	arg1	Dyes					64:67	Sulfonated Azo Dyes	49:67	Sulfonated Azo Dyes from Aqueous Solutions	49:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	0	72	from	Solutions	82:90	arg1	Removal					38:44	Removal	38:44	Removal of Sulfonated Azo Dyes from Aqueous Solutions	38:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	1	73	theme	materials	194:202	arg1	properties					167:176	the properties	163:176	the properties of final hybrid materials	163:202	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	6	74	theme	data	1094:1097	arg1	analysis					1062:1069	the analysis	1058:1069	the analysis of adsorption isotherm data	1058:1097	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	8	75	from	chitosan	1501:1508	arg1	processes					1528:1536	the adsorption processes	1513:1536	the adsorption processes	1513:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	76	theme	fractal-like	1651:1662	arg1	MOE					1664:1666	fractal-like MOE	1651:1666	fractal-like MOE	1651:1666	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	4	77	theme	=	767:767	arg1	surface					752:758	a more negatively charged surface	726:758	a more negatively charged surface (pHPZC = 4.2)	726:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	4	77	theme	=	767:767	arg1	pHPZC					761:765	pHPZC = 4.2	761:771	pHPZC = 4.2	761:771	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	11	78	theme	following	2147:2155	arg1	order					2157:2161	the following order	2143:2161	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	6	79	theme	adsorption	1074:1083	arg1	data					1094:1097	adsorption isotherm data	1074:1097	adsorption isotherm data	1074:1097	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	11	80	theme	>	2217:2217	arg1	halftimes					2231:2239	halftimes	2231:2239	halftimes	2231:2239	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	80	theme	>	2217:2217	arg1	AO8					2226:2228	acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8	2164:2228	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	3	81	theme	surface	548:554	arg1	area					556:559	specific surface area	539:559	specific surface area	539:559	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	10	82	theme	dye	1986:1988	arg1	molecules					1990:1998	dye molecules	1986:1998	dye molecules	1986:1998	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	7	83	theme	higher	1204:1209	arg1	capacity					1220:1227	a higher sorption capacity	1202:1227	a higher sorption capacity	1202:1227	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	8	84	from	processes	1528:1536	arg1	behavior					1477:1484	the behavior	1473:1484	the behavior of immobilized chitosan in the adsorption processes	1473:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	85	theme	chitosan	1501:1508	arg1	behavior					1477:1484	the behavior	1473:1484	the behavior of immobilized chitosan in the adsorption processes	1473:1536	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	1	86	theme	immobilization	138:151	arg1	method					153:158	the chitosan immobilization method	125:158	the chitosan immobilization method	125:158	In this study, the influence of the chitosan immobilization method on the properties of final hybrid materials was performed.
29345945	10	87	theme	internal	2009:2016	arg1	space					2018:2022	the internal space	2005:2022	the internal space of the pore structure	2005:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	11	88	theme	>	2224:2224	arg1	halftimes					2231:2239	halftimes	2231:2239	halftimes	2231:2239	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	88	theme	>	2224:2224	arg1	AO8					2226:2228	acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8	2164:2228	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	8	89	theme	diffusion	1702:1710	arg1	equations					1718:1726	pore diffusion model equations	1697:1726	pore diffusion model equations	1697:1726	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	8	90	theme	various	1539:1545	arg1	equations					1555:1563	various kinetic equations	1539:1563	various kinetic equations	1539:1563	For a deeper understanding of the behavior of immobilized chitosan in the adsorption processes, various kinetic equations were applied: first-order, second-order, mixed 1,2-order (MOE), multiexponential, and fractal-like MOE as well as intraparticle and pore diffusion model equations.
29345945	5	91	theme	dye	834:836	arg1	adsorption					838:847	azo dye adsorption	830:847	azo dye adsorption	830:847	The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished.
29345945	7	92	theme	AO8	1433:1435	arg1	dye					1437:1439	the AO8 dye	1429:1439	the AO8 dye	1429:1439	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	6	93	theme	Langmuir	947:954	arg1	equation					956:963	The generalized Langmuir equation	931:963	The generalized Langmuir equation	931:963	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	6	93	theme	Langmuir	947:954	arg1	Langmuir-Freundlich					997:1015	Langmuir-Freundlich	997:1015	Langmuir-Freundlich	997:1015	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	10	94	theme	pore	2031:2034	arg1	structure					2036:2044	the pore structure	2027:2044	the pore structure	2027:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	11	95	theme	ChS2	2100:2103	arg1	case					2092:2095	the case	2088:2095	the case of ChS2	2088:2103	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	4	96	theme	chitosan-silica	656:670	arg1	material					672:679	The microporous chitosan-silica material	640:679	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2)	640:772	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
29345945	0	97	theme	Hybrid	16:21	arg1	Composites					23:32	Chitosan-Silica Hybrid Composites	0:32	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.	0:91	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	10	98	theme	diffusion	1968:1976	arg1	rate					1978:1981	the diffusion rate	1964:1981	the diffusion rate of dye molecules into the internal space of the pore structure	1964:2044	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	11	99	theme	OG	2194:2195	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	99	theme	OG	2194:2195	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	9	100	theme	dye	1748:1750	arg1	case					1736:1739	the case	1732:1739	the case of AO8 dye	1732:1750	In the case of AO8 dye, the adsorption rates were differentiated for three composites: for ChS3, 50% of the dye was removed from the solution after merely 5 min and almost 90% after 80 min.
29345945	2	101	theme	sol-gel	335:341	arg1	ChS1					351:354	ChS1	351:354	ChS1	351:354	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	2	101	theme	sol-gel	335:341	arg1	method					343:348	the sol-gel method	331:348	the sol-gel method (ChS1)	331:355	Chitosan was immobilized on the surface of mesoporous (ChS2) and fumed silica (ChS3) by physical adsorption and the sol-gel method (ChS1).
29345945	11	102	theme	G	2191:2191	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	102	theme	G	2191:2191	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	103	theme	red	2205:2207	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	103	theme	red	2205:2207	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	3	104	theme	chitosan	403:410	arg1	immobilization					385:398	physical immobilization	376:398	physical immobilization of chitosan	376:410	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	0	105	theme	Azo	60:62	arg1	Dyes					64:67	Sulfonated Azo Dyes	49:67	Sulfonated Azo Dyes from Aqueous Solutions	49:90	Chitosan-Silica Hybrid Composites for Removal of Sulfonated Azo Dyes from Aqueous Solutions.
29345945	7	106	theme	acid	1317:1320	arg1	ChS1					1342:1345	ChS1	1342:1345	ChS1	1342:1345	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	106	theme	acid	1317:1320	arg1	dye					1337:1339	the acid orange 8 (AO8) dye	1313:1339	the acid orange 8 (AO8) dye (ChS1)	1313:1346	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	7	107	theme	adsorbed	1144:1151	arg1	chitosan					1153:1160	physically adsorbed chitosan	1133:1160	physically adsorbed chitosan (ChS1 and ChS2) on a silica surface	1133:1196	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	10	108	theme	min	2070:2072	arg1	halftime					2074:2081	225 min halftime	2066:2081	225 min halftime	2066:2081	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	10	108	theme	min	2070:2072	arg1	ChS1					2060:2063	ChS1	2060:2063	ChS1 (225 min halftime)	2060:2082	The slowest adsorption process controlled by the diffusion rate of dye molecules into the internal space of the pore structure was found for ChS1 (225 min halftime).
29345945	11	109	theme	>	2198:2198	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	109	theme	>	2198:2198	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	110	theme	orange	2169:2174	arg1	acid					2200:2203	acid orange (AO7) > orange G (OG) > acid red 1	2164:2209	the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min)	2143:2278	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	11	110	theme	orange	2169:2174	arg1	AR1					2212:2214	AR1	2212:2214	AR1	2212:2214	In the case of ChS2, the rates for various dyes change in the following order: acid orange (AO7) > orange G (OG) > acid red 1 (AR1) > AR88 > AO8 (halftimes: 10.5 < 15.7 < 23.7 < 34.9 < 42.9 min).
29345945	5	111	theme	adsorption	838:847	arg1	mechanisms					816:825	The mechanisms	812:825	The mechanisms of azo dye adsorption	812:847	The mechanisms of azo dye adsorption were studied, and the correlation with the composite structure was distinguished.
29345945	3	112	theme	hybrid	429:434	arg1	composites					436:445	hybrid composites	429:445	hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2	429:637	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	3	112	theme	hybrid	429:434	arg1	ChS					448:450	ChS	448:450	ChS	448:450	It was found that physical immobilization of chitosan allows to obtain hybrid composites (ChS) with a homogeneous distribution of polymer on the surface, relatively wide pores, and specific surface area of about 170 m2/g, pHPZC = 5.7 for ChS3 and 356 m2/g and pHPZC = 6.0 for ChS2.
29345945	6	113	theme	special	973:979	arg1	cases					981:985	its special cases	969:985	its special cases	969:985	The generalized Langmuir equation and its special cases, that is, Langmuir-Freundlich and Langmuir equations, were applied for the analysis of adsorption isotherm data.
29345945	7	114	theme	silica	1183:1188	arg1	surface					1190:1196	a silica surface	1181:1196	a silica surface	1181:1196	The adsorption study showed that physically adsorbed chitosan (ChS1 and ChS2) on a silica surface has a higher sorption capacity, for example, 0.48 mmol/g for the acid red 88 (AR88) dye (ChS2) and 0.23 mmol/g for the acid orange 8 (AO8) dye (ChS1), compared to the composite obtained by the sol-gel method [ChS1, 0.05 mmol/g for the AO8 dye].
29345945	4	115	theme	specific	688:695	arg1	area					705:708	a specific surface area	686:708	a specific surface area of 600 m2/g	686:720	The microporous chitosan-silica material with a specific surface area of 600 m2/g and a more negatively charged surface (pHPZC = 4.2) was obtained by the sol-gel reaction.
31881300	3	0	from	hydrogel	437:444	arg1	embedded					400:407	embedded	400:407	embedded	400:407	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	7	1	theme	composite	1012:1020	arg1	hydrogel					1022:1029	The composite hydrogel	1008:1029	The composite hydrogel	1008:1029	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	4	2	theme	obtained	552:559	arg1	release					561:567	the obtained release	548:567	the obtained release from microparticles and hydrogel	548:600	This composite hydrogel showed for both drugs a slower release than the obtained release from microparticles and hydrogel separately.
31881300	5	3	theme	Different	689:697	arg1	models					707:712	Different kinetic models	689:712	Different kinetic models	689:712	The release of both drugs was observed during a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion.
31881300	0	4	theme	dual	112:115	arg1	carrier					131:137	a dual drug delivery carrier	110:137	a dual drug delivery carrier	110:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	5	5	theme	kinetic	699:705	arg1	models					707:712	Different kinetic models	689:712	Different kinetic models	689:712	The release of both drugs was observed during a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion.
31881300	9	6	theme	wound	1464:1468	arg1	healing					1470:1476	wound healing	1464:1476	wound healing applications	1464:1489	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	3	7	from	embedded	400:407	arg1	hydrogel					437:444	a physically crosslinked hydrogel	412:444	a physically crosslinked hydrogel of κ-carrageenan/locust bean gum	412:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	9	8	theme	healing	1470:1476	arg1	applications					1478:1489	wound healing applications	1464:1489	wound healing applications	1464:1489	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	0	9	theme	delivery	122:129	arg1	carrier					131:137	a dual drug delivery carrier	110:137	a dual drug delivery carrier	110:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	6	10	theme	particle	924:931	arg1	size					933:936	average mean particle size	911:936	average mean particle size of 1.0 μm	911:946	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	1	11	theme	dual	185:188	arg1	carrier					204:210	a dual drug delivery carrier	183:210	a dual drug delivery carrier	183:210	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	1	11	theme	dual	185:188	arg1	hydrogel					158:165	A novel composite hydrogel	140:165	A novel composite hydrogel	140:165	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	0	12	theme	drug	117:120	arg1	carrier					131:137	a dual drug delivery carrier	110:137	a dual drug delivery carrier	110:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	0	13	from	embedded	56:63	arg1	hydrogel					98:105	κ-carrageenan/locust bean gum hydrogel	68:105	κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier	68:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	8	14	dep	higher	1174:1179	arg1	component					1198:1206	the viscous component	1186:1206	higher than the viscous component	1174:1206	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	6	15	theme	average	911:917	arg1	size					933:936	average mean particle size	911:936	average mean particle size of 1.0 μm	911:946	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	5	16	theme	7 days	672:677	arg1	period					662:667	a period	660:667	a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion	660:851	The release of both drugs was observed during a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion.
31881300	3	17	theme	bean	470:473	arg1	gum					475:477	κ-carrageenan/locust bean gum	449:477	κ-carrageenan/locust bean gum	449:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	6	18	theme	1.0 μm	941:946	arg1	size					933:936	average mean particle size	911:936	average mean particle size of 1.0 μm	911:946	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	6	19	theme	loaded	869:874	arg1	microparticles					876:889	the drug loaded microparticles	860:889	the drug loaded microparticles	860:889	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	3	20	theme	gum	475:477	arg1	hydrogel					437:444	a physically crosslinked hydrogel	412:444	a physically crosslinked hydrogel of κ-carrageenan/locust bean gum	412:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	5	21	theme	drugs	634:638	arg1	release					618:624	The release	614:624	The release of both drugs	614:638	The release of both drugs was observed during a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion.
31881300	1	22	theme	delivery	195:202	arg1	carrier					204:210	a dual drug delivery carrier	183:210	a dual drug delivery carrier	183:210	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	1	22	theme	delivery	195:202	arg1	hydrogel					158:165	A novel composite hydrogel	140:165	A novel composite hydrogel	140:165	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	4	23	from	hydrogel	593:600	arg1	release					561:567	the obtained release	548:567	the obtained release from microparticles and hydrogel	548:600	This composite hydrogel showed for both drugs a slower release than the obtained release from microparticles and hydrogel separately.
31881300	6	24	dep	loaded	869:874	arg1	drug					864:867	drug	864:867	drug	864:867	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	8	25	theme	hydrogel	1248:1255	arg1	characteristics					1220:1234	characteristics	1220:1234	characteristics of a strong hydrogel	1220:1255	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	6	26	theme	mean	919:922	arg1	size					933:936	average mean particle size	911:936	average mean particle size of 1.0 μm	911:946	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	7	27	theme	swelling	1091:1098	arg1	ratio					1100:1104	swelling ratio	1091:1104	swelling ratio	1091:1104	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	0	28	from	hydrogel	98:105	arg1	embedded					56:63	embedded	56:63	embedded	56:63	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	3	29	link	crosslinked	425:435	arg1	hydrogel					437:444	a physically crosslinked hydrogel	412:444	a physically crosslinked hydrogel of κ-carrageenan/locust bean gum	412:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	1	30	theme	drug	190:193	arg1	carrier					204:210	a dual drug delivery carrier	183:210	a dual drug delivery carrier	183:210	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	1	30	theme	drug	190:193	arg1	hydrogel					158:165	A novel composite hydrogel	140:165	A novel composite hydrogel	140:165	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	3	31	theme	κ-carrageenan/locust	449:468	arg1	gum					475:477	κ-carrageenan/locust bean gum	449:477	κ-carrageenan/locust bean gum	449:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	4	32	from	microparticles	574:587	arg1	release					561:567	the obtained release	548:567	the obtained release from microparticles and hydrogel	548:600	This composite hydrogel showed for both drugs a slower release than the obtained release from microparticles and hydrogel separately.
31881300	7	33	theme	swelling	1056:1063	arg1	behavior					1065:1072	a thermosensitive swelling behavior	1038:1072	a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C	1038:1113	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	5	34	theme	Higuchi	776:782	arg1	model					784:788	a Higuchi model	774:788	a Higuchi model suggesting that the release was mostly controlled by diffusion	774:851	The release of both drugs was observed during a period of 7 days at 37 °C. Different kinetic models were analyzed and the results indicated the best fitting to a Higuchi model suggesting that the release was mostly controlled by diffusion.
31881300	3	35	theme	crosslinked	425:435	arg1	hydrogel					437:444	a physically crosslinked hydrogel	412:444	a physically crosslinked hydrogel of κ-carrageenan/locust bean gum	412:477	These microparticles were embedded in a physically crosslinked hydrogel of κ-carrageenan/locust bean gum.
31881300	8	36	theme	viscous	1190:1196	arg1	component					1198:1206	the viscous component	1186:1206	higher than the viscous component	1174:1206	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	8	37	theme	strong	1241:1246	arg1	hydrogel					1248:1255	a strong hydrogel	1239:1255	a strong hydrogel	1239:1255	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	0	38	theme	bean	89:92	arg1	hydrogel					98:105	κ-carrageenan/locust bean gum hydrogel	68:105	κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier	68:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	4	39	theme	slower	528:533	arg1	release					535:541	a slower release	526:541	a slower release than the obtained release from microparticles and hydrogel	526:600	This composite hydrogel showed for both drugs a slower release than the obtained release from microparticles and hydrogel separately.
31881300	7	40	theme	ratio	1100:1104	arg1	%					1086:1086	183%	1083:1086	183% of swelling ratio	1083:1104	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	7	40	theme	ratio	1100:1104	arg1	ratio					1100:1104	swelling ratio	1091:1104	swelling ratio	1091:1104	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	9	41	theme	in	1298:1299	arg1	assays					1320:1325	in vitro cytotoxicity assays	1298:1325	in vitro cytotoxicity assays	1298:1325	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	2	42	theme	Poly	213:216	arg1	microparticles					261:274	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles	213:274	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles	213:274	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	2	43	theme	drug	361:364	arg1	models					366:371	hydrophobic drug models	349:371	hydrophobic drug models	349:371	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	2	43	theme	drug	361:364	arg1	mupirocin					335:343	mupirocin	335:343	mupirocin	335:343	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	2	43	theme	drug	361:364	arg1	ketoprofen					320:329	ketoprofen	320:329	ketoprofen	320:329	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	9	44	theme	composite	1363:1371	arg1	biomaterial					1417:1427	a potential biomaterial	1405:1427	a potential biomaterial for dual drug delivery	1405:1450	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	9	44	theme	composite	1363:1371	arg1	hydrogel					1373:1380	this composite hydrogel	1358:1380	this composite hydrogel	1358:1380	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	4	45	theme	composite	485:493	arg1	hydrogel					495:502	This composite hydrogel	480:502	This composite hydrogel	480:502	This composite hydrogel showed for both drugs a slower release than the obtained release from microparticles and hydrogel separately.
31881300	7	46	theme	thermosensitive	1040:1054	arg1	behavior					1065:1072	a thermosensitive swelling behavior	1038:1072	a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C	1038:1113	The composite hydrogel showed a thermosensitive swelling behavior reaching 183% of swelling ratio at 37 °C.
31881300	2	47	theme	hydrophobic	349:359	arg1	models					366:371	hydrophobic drug models	349:371	hydrophobic drug models	349:371	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	2	47	theme	hydrophobic	349:359	arg1	mupirocin					335:343	mupirocin	335:343	mupirocin	335:343	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	2	47	theme	hydrophobic	349:359	arg1	ketoprofen					320:329	ketoprofen	320:329	ketoprofen	320:329	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models.
31881300	9	48	theme	dual	1433:1436	arg1	delivery					1443:1450	dual drug delivery	1433:1450	dual drug delivery	1433:1450	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	8	49	theme	elastic	1150:1156	arg1	component					1158:1166	the elastic component	1146:1166	the elastic component to be higher than the viscous component	1146:1206	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	9	50	theme	cytotoxicity	1307:1318	arg1	assays					1320:1325	in vitro cytotoxicity assays	1298:1325	in vitro cytotoxicity assays	1298:1325	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	9	51	dep	in	1298:1299	arg1	vitro					1301:1305	vitro	1301:1305	vitro	1301:1305	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	1	52	theme	novel	142:146	arg1	hydrogel					158:165	A novel composite hydrogel	140:165	A novel composite hydrogel	140:165	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	1	52	theme	novel	142:146	arg1	carrier					204:210	a dual drug delivery carrier	183:210	a dual drug delivery carrier	183:210	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	9	53	theme	drug	1438:1441	arg1	delivery					1443:1450	dual drug delivery	1433:1450	dual drug delivery	1433:1450	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	0	54	theme	gum	94:96	arg1	hydrogel					98:105	κ-carrageenan/locust bean gum hydrogel	68:105	κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier	68:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	1	55	theme	composite	148:156	arg1	hydrogel					158:165	A novel composite hydrogel	140:165	A novel composite hydrogel	140:165	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	1	55	theme	composite	148:156	arg1	carrier					204:210	a dual drug delivery carrier	183:210	a dual drug delivery carrier	183:210	A novel composite hydrogel was prepared as a dual drug delivery carrier.
31881300	6	56	with	spherical	896:904	arg1	size					933:936	average mean particle size	911:936	average mean particle size of 1.0 μm	911:946	Also, the drug loaded microparticles were spherical with average mean particle size of 1.0 μm, mesoporous, and distributed homogeneously in the hydrogel.
31881300	0	57	theme	κ-carrageenan/locust	68:87	arg1	hydrogel					98:105	κ-carrageenan/locust bean gum hydrogel	68:105	κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier	68:137	Poly(hydroxybutyrate-co-hydroxyvalerate) microparticles embedded in κ-carrageenan/locust bean gum hydrogel as a dual drug delivery carrier.
31881300	8	58	theme	composite	1120:1128	arg1	hydrogel					1130:1137	The composite hydrogel	1116:1137	The composite hydrogel	1116:1137	The composite hydrogel showed the elastic component to be higher than the viscous component, indicating characteristics of a strong hydrogel.
31881300	9	59	theme	potential	1407:1415	arg1	biomaterial					1417:1427	a potential biomaterial	1405:1427	a potential biomaterial for dual drug delivery	1405:1450	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
31881300	9	59	theme	potential	1407:1415	arg1	hydrogel					1373:1380	this composite hydrogel	1358:1380	this composite hydrogel	1358:1380	The biocompatibility was evaluated with in vitro cytotoxicity assays and the results indicated that this composite hydrogel could be considered as a potential biomaterial for dual drug delivery, mainly for wound healing applications.
29534439	3	0	theme	therapeutics	771:782	arg1	delivery					784:791	intracellular therapeutics delivery	757:791	intracellular therapeutics delivery	757:791	However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery.
29534439	6	1	theme	alternate	1419:1427	arg1	deposition					1429:1438	the alternate deposition	1415:1438	the alternate deposition of both materials	1415:1456	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	6	2	theme	inexpensive	1191:1201	arg1	technology					1228:1237	the inexpensive and highly versatile LbL technology	1187:1237	the inexpensive and highly versatile LbL technology	1187:1237	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	1	3	theme	intracellular	288:300	arg1	trafficking					302:312	intracellular trafficking	288:312	intracellular trafficking	288:312	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	2	4	theme	sacrificial	441:451	arg1	templates					453:461	porous calcium carbonate sacrificial templates	416:461	porous calcium carbonate sacrificial templates	416:461	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	7	5	theme	advanced	1622:1629	arg1	techniques					1642:1651	advanced microscopy techniques	1622:1651	advanced microscopy techniques	1622:1651	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	5	6	theme	anionic	1000:1006	arg1	chitosan					981:988	Cationic chitosan	972:988	Cationic chitosan (CHT)	972:994	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	6	theme	anionic	1000:1006	arg1	ALG					1018:1020	ALG	1018:1020	ALG	1018:1020	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	6	theme	anionic	1000:1006	arg1	polysaccharides					1056:1070	the marine origin polysaccharides	1038:1070	the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues	1038:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	6	theme	anionic	1000:1006	arg1	alginate					1008:1015	anionic alginate	1000:1015	anionic alginate (ALG)	1000:1021	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	7	theme	tissues	1168:1174	arg1	matrices					1149:1156	the extracellular matrices	1131:1156	the extracellular matrices of living tissues	1131:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	7	8	theme	microscopy	1631:1640	arg1	techniques					1642:1651	advanced microscopy techniques	1622:1651	advanced microscopy techniques	1622:1651	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	5	9	theme	structural	1106:1115	arg1	similarity					1117:1126	their biocompatibility and structural similarity	1079:1126	similarity	1117:1126	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	4	10	theme	well-dispersed	831:844	arg1	microcapsules					887:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	5	11	theme	marine	1042:1047	arg1	chitosan					981:988	Cationic chitosan	972:988	Cationic chitosan (CHT)	972:994	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	11	theme	marine	1042:1047	arg1	polysaccharides					1056:1070	the marine origin polysaccharides	1038:1070	the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues	1038:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	11	theme	marine	1042:1047	arg1	alginate					1008:1015	anionic alginate	1000:1015	anionic alginate (ALG)	1000:1021	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	6	12	theme	core-shell	1261:1270	arg1	microparticles					1272:1285	core-shell microparticles	1261:1285	core-shell microparticles	1261:1285	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	8	13	theme	enhanced	1697:1704	arg1	uptake					1706:1711	an enhanced uptake	1694:1711	an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization	1694:1781	The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
29534439	6	14	theme	hollow	1291:1296	arg1	microcapsules					1311:1323	hollow multilayered microcapsules	1291:1323	hollow multilayered microcapsules	1291:1323	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	5	15	theme	origin	1049:1054	arg1	chitosan					981:988	Cationic chitosan	972:988	Cationic chitosan (CHT)	972:994	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	15	theme	origin	1049:1054	arg1	polysaccharides					1056:1070	the marine origin polysaccharides	1038:1070	the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues	1038:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	15	theme	origin	1049:1054	arg1	alginate					1008:1015	anionic alginate	1000:1015	anionic alginate (ALG)	1000:1021	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	6	16	used	used	1243:1246	arg2	technology					1228:1237	the inexpensive and highly versatile LbL technology	1187:1237	the inexpensive and highly versatile LbL technology	1187:1237	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	1	17	theme	drug	315:318	arg1	delivery					320:327	drug delivery	315:327	drug delivery	315:327	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	4	18	theme	optimized	940:948	arg1	process					963:969	an optimized purification process	937:969	an optimized purification process	937:969	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	4	19	theme	hollow	867:872	arg1	microcapsules					887:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	2	20	theme	calcium	423:429	arg1	templates					453:461	porous calcium carbonate sacrificial templates	416:461	porous calcium carbonate sacrificial templates	416:461	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	1	21	theme	biomedical	201:210	arg1	fields					230:235	the biomedical and biotechnology fields	197:235	the biomedical and biotechnology fields	197:235	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	2	22	theme	increasing	561:570	arg1	attention					572:580	increasing attention	561:580	increasing attention	561:580	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	7	23	theme	morphological	1586:1598	arg1	analysis					1600:1607	the morphological analysis	1582:1607	the morphological analysis monitored by advanced microscopy techniques	1582:1651	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	6	24	theme	LbL	1224:1226	arg1	technology					1228:1237	the inexpensive and highly versatile LbL technology	1187:1237	the inexpensive and highly versatile LbL technology	1187:1237	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	4	25	theme	polysaccharide-based	846:865	arg1	microcapsules					887:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	2	26	theme	carbonate	431:439	arg1	templates					453:461	porous calcium carbonate sacrificial templates	416:461	porous calcium carbonate sacrificial templates	416:461	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	2	27	theme	prominent	503:511	arg1	LbL					529:531	LbL	529:531	LbL	529:531	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	2	27	theme	prominent	503:511	arg1	Layer-by-Layer					513:526	the prominent Layer-by-Layer	499:526	the prominent Layer-by-Layer (LbL)	499:532	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	6	28	theme	physicochemical	1374:1388	arg1	properties					1390:1399	physicochemical properties	1374:1399	physicochemical properties	1374:1399	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	1	29	theme	Hollow	121:126	arg1	capsules					141:148	Hollow multilayered capsules	121:148	Hollow multilayered capsules	121:148	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	1	30	theme	biotechnology	216:228	arg1	fields					230:235	the biomedical and biotechnology fields	197:235	the biomedical and biotechnology fields	197:235	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	4	31	theme	purification	950:961	arg1	process					963:969	an optimized purification process	937:969	an optimized purification process	937:969	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	1	32	theme	multilayered	128:139	arg1	capsules					141:148	Hollow multilayered capsules	121:148	Hollow multilayered capsules	121:148	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	2	33	theme	porous	416:421	arg1	templates					453:461	porous calcium carbonate sacrificial templates	416:461	porous calcium carbonate sacrificial templates	416:461	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	0	34	theme	Chitosan/Alginate	30:46	arg1	Microcapsules					68:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	8	35	dep	fibroblasts	1716:1726	arg1	opening					1729:1735	opening	1729:1735	opening	1729:1735	The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
29534439	7	36	theme	natural	1540:1546	arg1	tendency					1560:1567	their natural aggregation tendency	1534:1567	their natural aggregation tendency	1534:1567	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	4	37	theme	LbL	918:920	arg1	technique					922:930	the LbL technique	914:930	the LbL technique	914:930	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	2	38	theme	building	479:486	arg1	blocks					488:493	natural-origin building blocks	464:493	natural-origin building blocks	464:493	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	0	39	theme	Well-Dispersed	15:28	arg1	Microcapsules					68:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	6	40	theme	multilayered	1298:1309	arg1	microcapsules					1311:1323	hollow multilayered microcapsules	1291:1323	hollow multilayered microcapsules	1291:1323	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	3	41	theme	major	699:703	arg1	hurdle					705:710	a major hurdle	697:710	a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery	697:791	However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery.
29534439	7	42	theme	aggregation	1548:1558	arg1	tendency					1560:1567	their natural aggregation tendency	1534:1567	their natural aggregation tendency	1534:1567	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	8	43	theme	new	1737:1739	arg1	perspectives					1741:1752	new perspectives	1737:1752	new perspectives for cellular internalization	1737:1781	The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
29534439	2	44	theme	natural-origin	464:477	arg1	blocks					488:493	natural-origin building blocks	464:493	natural-origin building blocks	464:493	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	0	45	theme	Multilayered	55:66	arg1	Microcapsules					68:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	1	46	theme	tissue	333:338	arg1	engineering					340:350	tissue engineering	333:350	tissue engineering	333:350	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	2	47	theme	key	597:599	arg1	features					601:608	their key features	591:608	their key features	591:608	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	3	48	theme	great	651:655	arg1	tendency					657:664	a great tendency	649:664	a great tendency	649:664	However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery.
29534439	0	49	theme	Hollow	48:53	arg1	Microcapsules					68:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules	15:80	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	6	50	theme	materials	1448:1456	arg1	deposition					1429:1438	the alternate deposition	1415:1438	the alternate deposition of both materials	1415:1456	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	0	51	theme	Microcapsules	68:80	arg1	Preparation					0:10	Preparation	0:10	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.	0:119	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	8	52	theme	well-dispersed	1658:1671	arg1	microcapsules					1673:1685	The well-dispersed microcapsules	1654:1685	The well-dispersed microcapsules	1654:1685	The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
29534439	5	53	theme	extracellular	1135:1147	arg1	matrices					1149:1156	the extracellular matrices	1131:1156	the extracellular matrices of living tissues	1131:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	1	54	theme	massive	161:167	arg1	potential					169:177	massive potential	161:177	massive potential for being used in the biomedical and biotechnology fields	161:235	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	8	55	theme	cellular	1758:1765	arg1	internalization					1767:1781	cellular internalization	1758:1781	cellular internalization	1758:1781	The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
29534439	3	56	theme	cellular	728:735	arg1	internalization					737:751	cellular internalization	728:751	cellular internalization	728:751	However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery.
29534439	4	57	theme	microcapsules	887:899	arg1	preparation					816:826	the preparation	812:826	the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules	812:899	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	2	58	theme	hollow	368:373	arg1	microcapsules					375:387	hollow microcapsules	368:387	hollow microcapsules	368:387	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
29534439	5	59	theme	due	1072:1074	arg1	chitosan					981:988	Cationic chitosan	972:988	Cationic chitosan (CHT)	972:994	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	59	theme	due	1072:1074	arg1	polysaccharides					1056:1070	the marine origin polysaccharides	1038:1070	the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues	1038:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	59	theme	due	1072:1074	arg1	alginate					1008:1015	anionic alginate	1000:1015	anionic alginate (ALG)	1000:1021	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	6	60	theme	precise	1331:1337	arg1	control					1339:1345	precise control	1331:1345	precise control over their composition and physicochemical properties	1331:1399	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	4	61	theme	multilayered	874:885	arg1	microcapsules					887:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	well-dispersed polysaccharide-based hollow multilayered microcapsules	831:899	Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process.
29534439	6	62	theme	versatile	1214:1222	arg1	technology					1228:1237	the inexpensive and highly versatile LbL technology	1187:1237	the inexpensive and highly versatile LbL technology	1187:1237	Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials.
29534439	1	63	theme	cellular	262:269	arg1	internalization					271:285	cellular internalization	262:285	cellular internalization	262:285	Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering.
29534439	0	64	theme	Cellular	95:102	arg1	Internalization					104:118	Enhanced Cellular Internalization	86:118	Enhanced Cellular Internalization	86:118	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	5	65	theme	living	1161:1166	arg1	tissues					1168:1174	living tissues	1161:1174	living tissues	1161:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	7	66	theme	synthesis	1478:1486	arg1	procedure					1488:1496	The microcapsules' synthesis procedure	1459:1496	The microcapsules' synthesis procedure	1459:1496	The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques.
29534439	5	67	theme	Cationic	972:979	arg1	polysaccharides					1056:1070	the marine origin polysaccharides	1038:1070	the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues	1038:1174	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	67	theme	Cationic	972:979	arg1	CHT					991:993	CHT	991:993	CHT	991:993	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	67	theme	Cationic	972:979	arg1	chitosan					981:988	Cationic chitosan	972:988	Cationic chitosan (CHT)	972:994	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	5	67	theme	Cationic	972:979	arg1	alginate					1008:1015	anionic alginate	1000:1015	anionic alginate (ALG)	1000:1021	Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues.
29534439	0	68	theme	Enhanced	86:93	arg1	Internalization					104:118	Enhanced Cellular Internalization	86:118	Enhanced Cellular Internalization	86:118	Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization.
29534439	3	69	theme	intracellular	757:769	arg1	delivery					784:791	intracellular therapeutics delivery	757:791	intracellular therapeutics delivery	757:791	However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery.
29534439	2	70	theme	resorting	403:411	arg1	technology					534:543	resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology	403:543	resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology	403:543	In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features.
30154352	0	0	theme	Simultaneous	89:100	arg1	Improvements					102:113	Simultaneous Improvements	89:113	Simultaneous Improvements on Body Fat Reduction and Fecal Parameters	89:156	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	2	1	theme	body	463:466	arg1	fat					468:470	body fat	463:470	body fat	463:470	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	4	2	theme	g/kg	754:757	arg1	weight					764:769	0.97 and 1.94 g/kg body weight	740:769	0.97 and 1.94 g/kg body weight	740:769	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	1	3	from	effects	187:193	arg1	parameters					349:358	fecal bacterial parameters	333:358	fecal bacterial parameters	333:358	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	1	3	from	effects	187:193	arg1	accumulation					316:327	body fat accumulation	307:327	body fat accumulation	307:327	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	2	4	dep	effectively	431:441	arg1	<					446:446	p < 0.05	444:451	p < 0.05	444:451	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	1	5	theme	resistant	257:265	arg1	maltodextrin					267:278	resistant maltodextrin	257:278	resistant maltodextrin (RMD)	257:284	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	1	5	theme	resistant	257:265	arg1	RMD					281:283	RMD	281:283	RMD	281:283	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	2	6	dep	higher	526:531	arg1	<					518:518	p < 0.05	516:523	p < 0.05	516:523	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	7	theme	perfringens	546:556	arg1	growth					571:576	retarded growth	562:576	retarded growth (p < 0.05) of the Bifidobacterium spp	562:614	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	7	theme	perfringens	546:556	arg1	<					581:581	p < 0.05	579:586	p < 0.05	579:586	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	7	theme	perfringens	546:556	arg1	growth					533:538	a significantly (p < 0.05) higher growth	499:538	a significantly (p < 0.05) higher growth of C. perfringens	499:556	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	6	8	theme	bacteria	1345:1352	arg1	profiles					1354:1361	the obesity and deteriorated fecal bacteria profiles	1310:1361	the obesity and deteriorated fecal bacteria profiles	1310:1361	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	1	9	theme	maltodextrin	267:278	arg1	mixture					214:220	a mixture	212:220	a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD)	212:284	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	4	10	theme	p	817:817	arg1	<					819:819	p < 0.05	817:824	p < 0.05	817:824	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	1	11	theme	fecal	333:337	arg1	parameters					349:358	fecal bacterial parameters	333:358	fecal bacterial parameters	333:358	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	6	12	theme	fecal	1339:1343	arg1	bacteria					1345:1352	deteriorated fecal bacteria	1326:1352	deteriorated fecal bacteria	1326:1352	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	0	13	dep	Fat	123:125	arg1	Reduction					127:135	Reduction	127:135	Reduction	127:135	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	5	14	theme	C.	1085:1086	arg1	count					1100:1104	the C. perfringens count	1081:1104	the C. perfringens count (-14.8%)	1081:1113	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	14	theme	C.	1085:1086	arg1	%					1112:1112	-14.8%	1107:1112	-14.8%	1107:1112	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	1	15	theme	bacterial	339:347	arg1	parameters					349:358	fecal bacterial parameters	333:358	fecal bacterial parameters	333:358	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	4	16	theme	body	759:762	arg1	weight					764:769	0.97 and 1.94 g/kg body weight	740:769	0.97 and 1.94 g/kg body weight	740:769	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	6	17	theme	deteriorated	1326:1337	arg1	bacteria					1345:1352	deteriorated fecal bacteria	1326:1352	deteriorated fecal bacteria	1326:1352	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	18	theme	amount	1251:1256	arg1	supplementation					1223:1237	A supplementation	1221:1237	A supplementation of adequate amount of FOS and RMD	1221:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	5	19	theme	Bifidobacterium	1140:1154	arg1	count					1156:1160	the total Bifidobacterium count	1130:1160	the total Bifidobacterium count (134.4%)	1130:1169	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	19	theme	Bifidobacterium	1140:1154	arg1	%					1168:1168	134.4%	1163:1168	134.4%	1163:1168	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	20	theme	fatty	1199:1203	arg1	acids					1205:1209	total fecal short chain fatty acids	1175:1209	total fecal short chain fatty acids (195.4%)	1175:1218	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	20	theme	fatty	1199:1203	arg1	%					1217:1217	195.4%	1212:1217	195.4%	1212:1217	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	2	21	theme	p	579:579	arg1	growth					571:576	retarded growth	562:576	retarded growth (p < 0.05) of the Bifidobacterium spp	562:614	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	21	theme	p	579:579	arg1	<					581:581	p < 0.05	579:586	p < 0.05	579:586	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	5	22	theme	high	1006:1009	arg1	dose					1011:1014	a high dose	1004:1014	a high dose	1004:1014	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	6	23	theme	adequate	1242:1249	arg1	RMD					1269:1271	RMD	1269:1271	RMD	1269:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	23	theme	adequate	1242:1249	arg1	FOS					1261:1263	FOS	1261:1263	FOS	1261:1263	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	23	theme	adequate	1242:1249	arg1	amount					1251:1256	adequate amount	1242:1256	adequate amount of FOS and RMD	1242:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	0	24	theme	Body	118:121	arg1	Fat					123:125	Body Fat Reduction and Fecal Parameters	118:156	Fat	123:125	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	6	25	theme	high	1370:1373	arg1	consumption					1379:1389	high fat consumption	1370:1389	high fat consumption	1370:1389	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	5	26	theme	fecal	1181:1185	arg1	acids					1205:1209	total fecal short chain fatty acids	1175:1209	total fecal short chain fatty acids (195.4%)	1175:1218	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	26	theme	fecal	1181:1185	arg1	%					1217:1217	195.4%	1212:1217	195.4%	1212:1217	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	27	theme	total	1134:1138	arg1	count					1156:1160	the total Bifidobacterium count	1130:1160	the total Bifidobacterium count (134.4%)	1130:1169	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	27	theme	total	1134:1138	arg1	%					1168:1168	134.4%	1163:1168	134.4%	1163:1168	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	4	28	theme	total	931:935	arg1	fat					942:944	total body fat	931:944	total body fat (-19.2%)	931:953	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	28	theme	total	931:935	arg1	%					952:952	-19.2%	947:952	-19.2%	947:952	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	6	29	theme	concreted	1288:1296	arg1	solution					1298:1305	a concreted solution	1286:1305	a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption	1286:1389	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	4	30	from	dose	734:737	arg1	incorporation					683:695	an incorporation	680:695	an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively)	680:784	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	5	31	theme	short	1187:1191	arg1	acids					1205:1209	total fecal short chain fatty acids	1175:1209	total fecal short chain fatty acids (195.4%)	1175:1218	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	31	theme	short	1187:1191	arg1	%					1217:1217	195.4%	1212:1217	195.4%	1212:1217	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	2	32	theme	spp	612:614	arg1	growth					571:576	retarded growth	562:576	retarded growth (p < 0.05) of the Bifidobacterium spp	562:614	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	32	theme	spp	612:614	arg1	<					581:581	p < 0.05	579:586	p < 0.05	579:586	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	32	theme	spp	612:614	arg1	growth					533:538	a significantly (p < 0.05) higher growth	499:538	a significantly (p < 0.05) higher growth of C. perfringens	499:556	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	4	33	theme	high	662:665	arg1	control					671:677	the high fat control	658:677	the high fat control	658:677	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	2	34	theme	retarded	562:569	arg1	growth					571:576	retarded growth	562:576	retarded growth (p < 0.05) of the Bifidobacterium spp	562:614	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	34	theme	retarded	562:569	arg1	<					581:581	p < 0.05	579:586	p < 0.05	579:586	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	35	theme	Bifidobacterium	596:610	arg1	spp					612:614	the Bifidobacterium spp	592:614	the Bifidobacterium spp	592:614	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	5	36	theme	total	1175:1179	arg1	acids					1205:1209	total fecal short chain fatty acids	1175:1209	total fecal short chain fatty acids (195.4%)	1175:1218	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	36	theme	total	1175:1179	arg1	%					1217:1217	195.4%	1212:1217	195.4%	1212:1217	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	37	theme	above	1043:1047	arg1	impacts					1061:1067	the above undesirable impacts	1039:1067	the above undesirable impacts	1039:1067	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	4	38	theme	visceral	870:877	arg1	fat					879:881	total visceral fat	864:881	total visceral fat (-17.4%)	864:890	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	38	theme	visceral	870:877	arg1	%					889:889	-17.4%	884:889	-17.4%	884:889	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	6	39	theme	obesity	1314:1320	arg1	profiles					1354:1361	the obesity and deteriorated fecal bacteria profiles	1310:1361	the obesity and deteriorated fecal bacteria profiles	1310:1361	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	4	40	theme	feed	840:843	arg1	efficiency					845:854	feed efficiency	840:854	feed efficiency (-16%)	840:861	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	40	theme	feed	840:843	arg1	%					860:860	-16%	857:860	-16%	857:860	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	2	41	theme	fat	409:411	arg1	consumption					413:423	high dietary fat consumption	396:423	high dietary fat consumption	396:423	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	1	42	from	parameters	349:358	arg1	rats					363:366	rats	363:366	rats	363:366	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	2	43	theme	p	516:516	arg1	<					518:518	p < 0.05	516:523	p < 0.05	516:523	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	5	44	dep	feeding	969:975	arg1	FOS					981:983	FOS	981:983	FOS	981:983	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	44	dep	feeding	969:975	arg1	mixture					993:999	RMD mixture	989:999	RMD mixture at a high dose	989:1014	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	4	45	theme	fat	667:669	arg1	control					671:677	the high fat control	658:677	the high fat control	658:677	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	2	46	theme	dietary	401:407	arg1	consumption					413:423	high dietary fat consumption	396:423	high dietary fat consumption	396:423	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	0	47	theme	Maltodextrin	49:60	arg1	Inclusion					0:8	Inclusion	0:8	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets	0:78	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	4	48	theme	significant	804:814	arg1	reduction					827:835	a significant (p < 0.05) reduction	802:835	a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%)	802:953	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	2	49	theme	high	396:399	arg1	consumption					413:423	high dietary fat consumption	396:423	high dietary fat consumption	396:423	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	0	50	theme	Resistant	39:47	arg1	Maltodextrin					49:60	Resistant Maltodextrin	39:60	Resistant Maltodextrin	39:60	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	0	51	theme	Fecal	141:145	arg1	Parameters					147:156	Fecal Parameters	141:156	Fecal Parameters	141:156	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	4	52	theme	mixture	716:722	arg1	incorporation					683:695	an incorporation	680:695	an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively)	680:784	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	5	53	dep	C.	1085:1086	arg1	perfringens					1088:1098	perfringens	1088:1098	perfringens	1088:1098	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	1	54	theme	fructooligosaccharide	225:245	arg1	mixture					214:220	a mixture	212:220	a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD)	212:284	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	0	55	theme	High	65:68	arg1	Diets					74:78	High Fat Diets	65:78	High Fat Diets	65:78	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	1	56	theme	1:2	300:302	arg1	ratio					291:295	a ratio	289:295	a ratio of 1:2	289:302	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	6	57	theme	fat	1375:1377	arg1	consumption					1379:1389	high fat consumption	1370:1389	high fat consumption	1370:1389	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	4	58	theme	high	729:732	arg1	dose					734:737	a high dose	727:737	a high dose (0.97 and 1.94 g/kg body weight, respectively)	727:784	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	1	59	from	accumulation	316:327	arg1	rats					363:366	rats	363:366	rats	363:366	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	2	60	theme	higher	526:531	arg1	growth					533:538	a significantly (p < 0.05) higher growth	499:538	a significantly (p < 0.05) higher growth of C. perfringens	499:556	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	2	61	theme	p	444:444	arg1	<					446:446	p < 0.05	444:451	p < 0.05	444:451	Our results indicated that high dietary fat consumption might effectively (p < 0.05) increase body fat, but consequently inducing a significantly (p < 0.05) higher growth of C. perfringens and retarded growth (p < 0.05) of the Bifidobacterium spp.
30154352	5	62	dep	FOS	981:983	arg1	the					977:979	the	977:979	the	977:979	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	3	63	theme	large	624:628	arg1	intestine					630:638	the large intestine	620:638	the large intestine	620:638	in the large intestine.
30154352	1	64	theme	body	307:310	arg1	fat					312:314	body fat	307:314	body fat accumulation	307:327	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	4	65	dep	dose	734:737	arg1	weight					764:769	0.97 and 1.94 g/kg body weight	740:769	0.97 and 1.94 g/kg body weight	740:769	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	66	theme	total	864:868	arg1	fat					879:881	total visceral fat	864:881	total visceral fat (-17.4%)	864:890	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	66	theme	total	864:868	arg1	%					889:889	-17.4%	884:889	-17.4%	884:889	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	67	theme	FOS	704:706	arg1	mixture					716:722	the FOS and RMD mixture	700:722	mixture	716:722	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	6	68	theme	FOS	1261:1263	arg1	RMD					1269:1271	RMD	1269:1271	RMD	1269:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	68	theme	FOS	1261:1263	arg1	FOS					1261:1263	FOS	1261:1263	FOS	1261:1263	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	68	theme	FOS	1261:1263	arg1	amount					1251:1256	adequate amount	1242:1256	adequate amount of FOS and RMD	1242:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	5	69	from	dose	1011:1014	arg1	FOS					981:983	FOS	981:983	FOS	981:983	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	69	from	dose	1011:1014	arg1	mixture					993:999	RMD mixture	989:999	RMD mixture at a high dose	989:1014	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	6	70	theme	RMD	1269:1271	arg1	RMD					1269:1271	RMD	1269:1271	RMD	1269:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	70	theme	RMD	1269:1271	arg1	FOS					1261:1263	FOS	1261:1263	FOS	1261:1263	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	6	70	theme	RMD	1269:1271	arg1	amount					1251:1256	adequate amount	1242:1256	adequate amount of FOS and RMD	1242:1271	A supplementation of adequate amount of FOS and RMD might confer a concreted solution to the obesity and deteriorated fecal bacteria profiles due to high fat consumption.
30154352	5	71	theme	undesirable	1049:1059	arg1	impacts					1061:1067	the above undesirable impacts	1039:1067	the above undesirable impacts	1039:1067	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	4	72	theme	fat	906:908	arg1	levels					910:915	non-visceral fat levels	893:915	non-visceral fat levels (-20.3%)	893:924	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	72	theme	fat	906:908	arg1	%					923:923	-20.3%	918:923	-20.3%	918:923	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	5	73	theme	RMD	989:991	arg1	mixture					993:999	RMD mixture	989:999	RMD mixture at a high dose	989:1014	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	0	74	from	Improvements	102:113	arg1	Parameters					147:156	Fecal Parameters	141:156	Fecal Parameters	141:156	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	0	74	from	Improvements	102:113	arg1	Fat					123:125	Body Fat Reduction and Fecal Parameters	118:156	Fat	123:125	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	4	75	theme	RMD	712:714	arg1	mixture					716:722	the FOS and RMD mixture	700:722	mixture	716:722	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	76	theme	body	937:940	arg1	fat					942:944	total body fat	931:944	total body fat (-19.2%)	931:953	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	76	theme	body	937:940	arg1	%					952:952	-19.2%	947:952	-19.2%	947:952	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	77	dep	significant	804:814	arg1	<					819:819	p < 0.05	817:824	p < 0.05	817:824	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	78	theme	non-visceral	893:904	arg1	levels					910:915	non-visceral fat levels	893:915	non-visceral fat levels (-20.3%)	893:924	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	78	theme	non-visceral	893:904	arg1	%					923:923	-20.3%	918:923	-20.3%	918:923	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	fat					879:881	total visceral fat	864:881	total visceral fat (-17.4%)	864:890	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	%					860:860	-16%	857:860	-16%	857:860	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	%					952:952	-19.2%	947:952	-19.2%	947:952	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	levels					910:915	non-visceral fat levels	893:915	non-visceral fat levels (-20.3%)	893:924	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	%					889:889	-17.4%	884:889	-17.4%	884:889	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	%					923:923	-20.3%	918:923	-20.3%	918:923	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	fat					942:944	total body fat	931:944	total body fat (-19.2%)	931:953	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	4	79	from	reduction	827:835	arg1	efficiency					845:854	feed efficiency	840:854	feed efficiency (-16%)	840:861	As compared with the high fat control, an incorporation of the FOS and RMD mixture at a high dose (0.97 and 1.94 g/kg body weight, respectively) could result in a significant (p < 0.05) reduction in feed efficiency (-16%), total visceral fat (-17.4%), non-visceral fat levels (-20.3%), and total body fat (-19.2%).
30154352	0	80	theme	Fat	70:72	arg1	Diets					74:78	High Fat Diets	65:78	High Fat Diets	65:78	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	1	81	theme	fat	312:314	arg1	accumulation					316:327	body fat accumulation	307:327	body fat accumulation	307:327	This study investigated the effects of incorporating a mixture of fructooligosaccharide (FOS) and resistant maltodextrin (RMD) at a ratio of 1:2 on body fat accumulation and fecal bacterial parameters in rats.
30154352	0	82	theme	Fructooligosaccharide	13:33	arg1	Inclusion					0:8	Inclusion	0:8	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets	0:78	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	0	83	from	Inclusion	0:8	arg1	Diets					74:78	High Fat Diets	65:78	High Fat Diets	65:78	Inclusion of Fructooligosaccharide and Resistant Maltodextrin in High Fat Diets Promotes Simultaneous Improvements on Body Fat Reduction and Fecal Parameters.
30154352	5	84	theme	chain	1193:1197	arg1	acids					1205:1209	total fecal short chain fatty acids	1175:1209	total fecal short chain fatty acids (195.4%)	1175:1218	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
30154352	5	84	theme	chain	1193:1197	arg1	%					1217:1217	195.4%	1212:1217	195.4%	1212:1217	Furthermore, feeding the FOS and RMD mixture at a high dose was capable to counter the above undesirable impacts by reducing the C. perfringens count (-14.8%) and increasing the total Bifidobacterium count (134.4%) and total fecal short chain fatty acids (195.4%).
31865422	12	0	theme	relative	1261:1268	arg1	higher					1312:1317	higher	1312:1317	higher	1312:1317	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	12	0	theme	relative	1261:1268	arg1	abundance					1270:1278	The relative abundance	1257:1278	The relative abundance of Firmicutes	1257:1292	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	11	1	theme	LDLR	1216:1219	arg1	gene					1221:1224	the LDLR gene	1212:1224	the LDLR gene	1212:1224	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	7	2	theme	genes	756:760	arg1	Expression					742:751	Expression	742:751	Expression of genes involved in fatty acid and cholesterol metabolism	742:810	Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR.
31865422	10	3	theme	cholesterol	1058:1068	arg1	levels					1090:1095	serum total cholesterol and LDL cholesterol levels	1046:1095	serum total cholesterol and LDL cholesterol levels	1046:1095	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	8	4	theme	gut	860:862	arg1	composition					875:885	The gut microbiota composition	856:885	The gut microbiota composition	856:885	The gut microbiota composition was analysed by 16S rRNA gene sequencing.
31865422	13	5	theme	acid-producing	1405:1418	arg1	bacteria					1424:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	14	6	theme	eryngii	1537:1543	arg1	polysaccharides					1545:1559	P. eryngii polysaccharides	1534:1559	P. eryngii polysaccharides	1534:1559	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	14	7	dep	CONCLUSIONS	1476:1486	arg1	provide					1502:1508	provide	1502:1508	provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota	1502:1704	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	13	8	theme	bacteria	1424:1431	arg1	abundance					1369:1377	The abundance	1365:1377	The abundance of some short-chain fatty acid-producing gut bacteria	1365:1431	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	0	9	theme	high-fat	102:109	arg1	diet					111:114	a high-fat diet	100:114	a high-fat diet	100:114	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	14	10	theme	P.	1534:1535	arg1	polysaccharides					1545:1559	P. eryngii polysaccharides	1534:1559	P. eryngii polysaccharides	1534:1559	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	13	11	theme	short-chain	1387:1397	arg1	bacteria					1424:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	1	12	theme	activities	186:195	arg1	variety					158:164	a variety	156:164	a variety of health-promoting activities	156:195	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	1	12	theme	activities	186:195	arg1	activities					186:195	health-promoting activities	169:195	health-promoting activities	169:195	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	0	13	from	obesity	61:67	arg1	mice					91:94	mice	91:94	mice	91:94	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	11	14	theme	increased	1188:1196	arg1	expression					1198:1207	increased expression	1188:1207	increased expression of the LDLR gene in the liver and GPR43	1188:1247	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	5	15	theme	C57BL/6J	530:537	arg1	mice					539:542	C57BL/6J mice	530:542	C57BL/6J mice	530:542	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	9	16	dep	RESULTS	929:935	arg1	tissue					973:978	mesenteric fat tissue	958:978	mesenteric fat tissue	958:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	16	dep	RESULTS	929:935	arg1	RESULTS					929:935	RESULTS	929:935	RESULTS Body weight gain and mesenteric fat tissue	929:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	16	dep	RESULTS	929:935	arg1	gain					949:952	Body weight gain	937:952	Body weight gain	937:952	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	13	17	theme	fatty	1399:1403	arg1	bacteria					1424:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	1	18	theme	PURPOSE	117:123	arg1	Mushrooms					125:133	PURPOSE Mushrooms	117:133	PURPOSE Mushrooms	117:133	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	12	19	theme	ST	1355:1356	arg1	group					1358:1362	the ST group	1351:1362	the ST group	1351:1362	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	10	20	theme	LDL	1074:1076	arg1	cholesterol					1078:1088	LDL cholesterol	1074:1088	LDL cholesterol	1074:1088	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	14	21	theme	lipids	1676:1681	arg1	excretion					1648:1656	increased excretion	1638:1656	increased excretion of bile acids and lipids	1638:1681	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	14	21	theme	lipids	1676:1681	arg1	microbiota					1695:1704	altered microbiota	1687:1704	altered microbiota	1687:1704	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	9	22	theme	weight	942:947	arg1	RESULTS					929:935	RESULTS	929:935	RESULTS Body weight gain and mesenteric fat tissue	929:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	22	theme	weight	942:947	arg1	gain					949:952	Body weight gain	937:952	Body weight gain	937:952	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	0	23	theme	gut	73:75	arg1	microbiota					77:86	gut microbiota	73:86	gut microbiota	73:86	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	11	24	from	expression	1198:1207	arg1	liver					1233:1237	liver	1233:1237	liver	1233:1237	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	11	24	from	expression	1198:1207	arg1	GPR43					1243:1247	GPR43	1243:1247	GPR43	1243:1247	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	8	25	theme	gene	912:915	arg1	sequencing					917:926	rRNA gene sequencing	907:926	rRNA gene sequencing	907:926	The gut microbiota composition was analysed by 16S rRNA gene sequencing.
31865422	14	26	theme	acids	1666:1670	arg1	excretion					1648:1656	increased excretion	1638:1656	increased excretion of bile acids and lipids	1638:1681	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	14	26	theme	acids	1666:1670	arg1	microbiota					1695:1704	altered microbiota	1687:1704	altered microbiota	1687:1704	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	0	27	from	Effect	0:5	arg1	obesity					61:67	obesity	61:67	obesity	61:67	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	0	27	from	Effect	0:5	arg1	microbiota					77:86	gut microbiota	73:86	gut microbiota	73:86	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	0	27	from	Effect	0:5	arg1	eryngii					50:56	Pleurotus eryngii	40:56	Pleurotus eryngii	40:56	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	5	28	theme	eryngii	620:626	arg1	fraction					643:650	P. eryngii polysaccharide fraction	617:650	5% P. eryngii polysaccharide fraction (LP or HP)	614:661	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	7	29	theme	quantitative	838:849	arg1	PCR					851:853	real-time quantitative PCR	828:853	real-time quantitative PCR	828:853	Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR.
31865422	14	30	theme	altered	1687:1693	arg1	microbiota					1695:1704	altered microbiota	1687:1704	altered microbiota	1687:1704	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	0	31	from	eryngii	50:56	arg1	Effect					0:5	Effect	0:5	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice	0:94	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	0	31	from	eryngii	50:56	arg1	polysaccharides					19:33	mushroom polysaccharides	10:33	mushroom polysaccharides from Pleurotus eryngii	10:56	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	9	32	theme	fat	969:971	arg1	tissue					973:978	mesenteric fat tissue	958:978	mesenteric fat tissue	958:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	32	theme	fat	969:971	arg1	RESULTS					929:935	RESULTS	929:935	RESULTS Body weight gain and mesenteric fat tissue	929:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	10	33	theme	serum	1046:1050	arg1	cholesterol					1058:1068	serum total cholesterol	1046:1068	serum total cholesterol	1046:1068	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	14	34	theme	increased	1638:1646	arg1	excretion					1648:1656	increased excretion	1638:1656	increased excretion of bile acids and lipids	1638:1681	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	10	35	theme	total	1122:1126	arg1	acids					1133:1137	total bile acids	1122:1137	total bile acids	1122:1137	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	14	36	theme	bile	1661:1664	arg1	acids					1666:1670	bile acids	1661:1670	bile acids	1661:1670	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	13	37	theme	eryngii	1451:1457	arg1	polysaccharides					1459:1473	P. eryngii polysaccharides	1448:1473	P. eryngii polysaccharides	1448:1473	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	0	38	theme	mushroom	10:17	arg1	polysaccharides					19:33	mushroom polysaccharides	10:33	mushroom polysaccharides from Pleurotus eryngii	10:56	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	13	39	theme	gut	1420:1422	arg1	bacteria					1424:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	some short-chain fatty acid-producing gut bacteria	1382:1431	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	12	40	theme	HF	1326:1327	arg1	groups					1336:1341	the HF and HP groups	1322:1341	the HF and HP groups	1322:1341	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	2	41	from	effects	246:252	arg1	obesity					309:315	obesity	309:315	obesity	309:315	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	2	41	from	effects	246:252	arg1	eryngii					298:304	Pleurotus eryngii	288:304	Pleurotus eryngii	288:304	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	9	42	theme	HP	998:999	arg1	group					1001:1005	the HP group	994:1005	the HP group	994:1005	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	14	43	contain	have	1561:1564	arg2	effects					1608:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	14	43	contain	have	1561:1564	arg1	polysaccharides					1545:1559	P. eryngii polysaccharides	1534:1559	P. eryngii polysaccharides	1534:1559	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	12	44	theme	HP	1333:1334	arg1	groups					1336:1341	the HF and HP groups	1322:1341	the HF and HP groups	1322:1341	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	3	45	theme	eryngii	367:373	arg1	polysaccharides					375:389	P. eryngii polysaccharides	364:389	P. eryngii polysaccharides	364:389	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	1	46	contain	have	151:154	arg2	activities					186:195	health-promoting activities	169:195	health-promoting activities	169:195	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	1	46	contain	have	151:154	arg1	Mushrooms					125:133	PURPOSE Mushrooms	117:133	PURPOSE Mushrooms	117:133	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	1	46	contain	have	151:154	arg2	variety					158:164	a variety	156:164	a variety of health-promoting activities	156:195	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	14	47	theme	obese	1619:1623	arg1	mice					1625:1628	obese mice	1619:1628	obese mice	1619:1628	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	6	48	theme	blood	710:714	arg1	parameters					716:725	blood parameters	710:725	blood parameters	710:725	Adipose tissues were weighed and blood parameters were measured.
31865422	5	49	dep	diet	564:567	arg1	ST					570:571	ST	570:571	ST	570:571	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	11	50	theme	gene	1221:1224	arg1	expression					1198:1207	increased expression	1188:1207	increased expression of the LDLR gene in the liver and GPR43	1188:1247	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	10	51	theme	HP	1036:1037	arg1	group					1039:1043	the HP group	1032:1043	the HP group	1032:1043	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	7	52	theme	fatty	774:778	arg1	acid					780:783	fatty acid	774:783	fatty acid	774:783	Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR.
31865422	14	53	theme	anti-obesity	1566:1577	arg1	effects					1608:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	12	54	theme	Firmicutes	1283:1292	arg1	higher					1312:1317	higher	1312:1317	higher	1312:1317	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	12	54	theme	Firmicutes	1283:1292	arg1	abundance					1270:1278	The relative abundance	1257:1278	The relative abundance of Firmicutes	1257:1292	The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group.
31865422	8	55	theme	microbiota	864:873	arg1	composition					875:885	The gut microbiota composition	856:885	The gut microbiota composition	856:885	The gut microbiota composition was analysed by 16S rRNA gene sequencing.
31865422	2	56	theme	polysaccharides	267:281	arg1	intake					257:262	intake	257:262	intake of polysaccharides from Pleurotus eryngii	257:304	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	10	57	theme	total	1052:1056	arg1	cholesterol					1058:1068	serum total cholesterol	1046:1068	serum total cholesterol	1046:1068	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	6	58	theme	Adipose	677:683	arg1	tissues					685:691	Adipose tissues	677:691	Adipose tissues	677:691	Adipose tissues were weighed and blood parameters were measured.
31865422	2	59	theme	intake	257:262	arg1	effects					246:252	the effects	242:252	the effects of intake of polysaccharides from Pleurotus eryngii on obesity	242:315	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	11	60	theme	HP	1172:1173	arg1	group					1175:1179	the HP group	1168:1179	the HP group	1168:1179	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	5	61	theme	high-fat	577:584	arg1	HF					592:593	HF	592:593	HF	592:593	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	5	61	theme	high-fat	577:584	arg1	diet					586:589	a high-fat diet	575:589	a high-fat diet (HF)	575:594	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	8	62	theme	rRNA	907:910	arg1	sequencing					917:926	rRNA gene sequencing	907:926	rRNA gene sequencing	907:926	The gut microbiota composition was analysed by 16S rRNA gene sequencing.
31865422	1	63	theme	health-promoting	169:184	arg1	activities					186:195	health-promoting activities	169:195	health-promoting activities	169:195	PURPOSE Mushrooms are reported to have a variety of health-promoting activities.
31865422	0	64	from	microbiota	77:86	arg1	mice					91:94	mice	91:94	mice	91:94	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	10	65	from	lipid	1112:1116	arg1	faeces					1142:1147	faeces	1142:1147	faeces	1142:1147	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	14	66	theme	first	1514:1518	arg1	evidence					1520:1527	the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota	1510:1704	the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota	1510:1704	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	2	67	theme	little	207:212	arg1	information					214:224	little information	207:224	little information	207:224	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	10	68	from	acids	1133:1137	arg1	faeces					1142:1147	faeces	1142:1147	faeces	1142:1147	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	10	69	theme	cholesterol	1078:1088	arg1	levels					1090:1095	serum total cholesterol and LDL cholesterol levels	1046:1095	serum total cholesterol and LDL cholesterol levels	1046:1095	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	5	70	dep	%	615:615	arg1	LP					653:654	LP	653:654	LP	653:654	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	5	70	dep	%	615:615	arg1	fraction					643:650	P. eryngii polysaccharide fraction	617:650	5% P. eryngii polysaccharide fraction (LP or HP)	614:661	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	5	70	dep	%	615:615	arg1	HP					659:660	HP	659:660	HP	659:660	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	9	71	theme	Body	937:940	arg1	RESULTS					929:935	RESULTS	929:935	RESULTS Body weight gain and mesenteric fat tissue	929:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	71	theme	Body	937:940	arg1	gain					949:952	Body weight gain	937:952	Body weight gain	937:952	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	5	72	theme	P.	617:618	arg1	fraction					643:650	P. eryngii polysaccharide fraction	617:650	5% P. eryngii polysaccharide fraction (LP or HP)	614:661	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	3	73	from	obesity	394:400	arg1	mice					424:427	mice	424:427	mice fed a high-fat diet	424:447	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	2	74	from	eryngii	298:304	arg1	intake					257:262	intake	257:262	intake of polysaccharides from Pleurotus eryngii	257:304	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	2	74	from	eryngii	298:304	arg1	effects					246:252	the effects	242:252	the effects of intake of polysaccharides from Pleurotus eryngii on obesity	242:315	However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity.
31865422	9	75	theme	mesenteric	958:967	arg1	tissue					973:978	mesenteric fat tissue	958:978	mesenteric fat tissue	958:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	9	75	theme	mesenteric	958:967	arg1	RESULTS					929:935	RESULTS	929:935	RESULTS Body weight gain and mesenteric fat tissue	929:978	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	5	76	theme	polysaccharide	628:641	arg1	fraction					643:650	P. eryngii polysaccharide fraction	617:650	5% P. eryngii polysaccharide fraction (LP or HP)	614:661	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	3	77	from	effects	353:359	arg1	microbiota					410:419	gut microbiota	406:419	gut microbiota	406:419	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	3	77	from	effects	353:359	arg1	obesity					394:400	obesity	394:400	obesity	394:400	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	4	78	theme	Soluble	458:464	arg1	polysaccharides					466:480	Soluble polysaccharides	458:480	METHODS Soluble polysaccharides	450:480	METHODS Soluble polysaccharides were extracted from P. eryngii using hot water.
31865422	10	79	theme	bile	1128:1131	arg1	acids					1133:1137	total bile acids	1122:1137	total bile acids	1122:1137	In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased.
31865422	7	80	theme	real-time	828:836	arg1	PCR					851:853	real-time quantitative PCR	828:853	real-time quantitative PCR	828:853	Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR.
31865422	3	81	theme	P.	364:365	arg1	polysaccharides					375:389	P. eryngii polysaccharides	364:389	P. eryngii polysaccharides	364:389	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	3	82	theme	gut	406:408	arg1	microbiota					410:419	gut microbiota	406:419	gut microbiota	406:419	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	0	83	theme	polysaccharides	19:33	arg1	Effect					0:5	Effect	0:5	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice	0:94	Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet.
31865422	4	84	dep	METHODS	450:456	arg1	polysaccharides					466:480	Soluble polysaccharides	458:480	METHODS Soluble polysaccharides	450:480	METHODS Soluble polysaccharides were extracted from P. eryngii using hot water.
31865422	5	85	theme	standard	555:562	arg1	diet					564:567	a standard diet	553:567	a standard diet (ST)	553:572	C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks.
31865422	3	86	theme	high-fat	435:442	arg1	diet					444:447	a high-fat diet	433:447	a high-fat diet	433:447	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	11	87	from	Mice	1160:1163	arg1	group					1175:1179	the HP group	1168:1179	the HP group	1168:1179	Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat.
31865422	3	88	theme	polysaccharides	375:389	arg1	effects					353:359	the effects	349:359	the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet	349:447	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31865422	4	89	theme	hot	519:521	arg1	water					523:527	hot water	519:527	hot water	519:527	METHODS Soluble polysaccharides were extracted from P. eryngii using hot water.
31865422	7	90	theme	cholesterol	789:799	arg1	metabolism					801:810	cholesterol metabolism	789:810	cholesterol metabolism	789:810	Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR.
31865422	13	91	theme	P.	1448:1449	arg1	polysaccharides					1459:1473	P. eryngii polysaccharides	1448:1473	P. eryngii polysaccharides	1448:1473	The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides.
31865422	9	92	theme	HF	1019:1020	arg1	group					1022:1026	the HF group	1015:1026	the HF group	1015:1026	RESULTS Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group.
31865422	14	93	theme	cholesterol-lowering	1587:1606	arg1	effects					1608:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	anti-obesity and LDL cholesterol-lowering effects	1566:1614	CONCLUSIONS These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterol-lowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota.
31865422	3	94	from	microbiota	410:419	arg1	mice					424:427	mice	424:427	mice fed a high-fat diet	424:447	In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet.
31295479	0	0	theme	HeLa	84:87	arg1	cells					89:93	human cervical adenocarcinoma HeLa cells	54:93	human cervical adenocarcinoma HeLa cells	54:93	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	0	1	from	induction	9:17	arg1	cells					89:93	human cervical adenocarcinoma HeLa cells	54:93	human cervical adenocarcinoma HeLa cells	54:93	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	4	2	theme	normal	688:693	arg1	lines					700:704	investigated normal cell lines	675:704	investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney)	675:757	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	0	3	theme	adenocarcinoma	69:82	arg1	cells					89:93	human cervical adenocarcinoma HeLa cells	54:93	human cervical adenocarcinoma HeLa cells	54:93	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	5	4	theme	stress	871:876	arg1	sensors					878:884	ER stress sensors	868:884	ER stress sensors	868:884	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	4	5	theme	investigated	675:686	arg1	lines					700:704	investigated normal cell lines	675:704	investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney)	675:757	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	5	6	theme	malfolded	970:978	arg1	proteins					980:987	malfolded proteins	970:987	malfolded proteins	970:987	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	8	7	theme	HeLa	1427:1430	arg1	cells					1432:1436	HeLa cells	1427:1436	HeLa cells proliferation	1427:1450	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	2	8	theme	Reticulum	283:291	arg1	death					318:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	4	9	theme	200-600 μg/ml	562:574	arg1	concentrations					576:589	200-600 μg/ml concentrations	562:589	200-600 μg/ml concentrations of fucoidan	562:601	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	4	10	dep	HaCaT	707:711	arg1	HEK-293					732:738	HEK-293	732:738	HEK-293	732:738	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	4	10	dep	HaCaT	707:711	arg1	kidney					751:756	embryonic kidney	741:756	HaCaT: Keratinocytes and HEK-293: embryonic kidney	707:756	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	4	10	dep	HaCaT	707:711	arg1	Keratinocytes					714:726	Keratinocytes	714:726	Keratinocytes	714:726	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	3	11	theme	ER-stress-related	512:528	arg1	death					535:539	ER-stress-related cell death	512:539	ER-stress-related cell death	512:539	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	6	12	theme	ER	1181:1182	arg1	apoptosis					1199:1207	the ER stress-induced apoptosis	1177:1207	the ER stress-induced apoptosis	1177:1207	In addition, intracellular calcium levels were elevated following the treatment suggesting that this contributed to the ER stress-induced apoptosis.
31295479	2	13	theme	Endoplasmic	271:281	arg1	ER					294:295	ER	294:295	ER	294:295	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	2	13	theme	Endoplasmic	271:281	arg1	Reticulum					283:291	Endoplasmic Reticulum	271:291	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	2	14	theme	cell	313:316	arg1	death					318:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	8	15	theme	mitochondrial-mediated	1507:1528	arg1	apoptosis					1530:1538	mitochondrial-mediated apoptosis	1507:1538	mitochondrial-mediated apoptosis	1507:1538	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	3	16	theme	cells	492:496	arg1	death					498:502	HeLa cells death	487:502	HeLa cells death	487:502	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	1	17	theme	cell-wall	163:171	arg1	composition					173:183	cell-wall composition	163:183	cell-wall composition of brown algae	163:198	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	6	18	theme	calcium	1088:1094	arg1	levels					1096:1101	intracellular calcium levels	1074:1101	intracellular calcium levels	1074:1101	In addition, intracellular calcium levels were elevated following the treatment suggesting that this contributed to the ER stress-induced apoptosis.
31295479	5	19	theme	Bip/GRP78	914:922	arg1	upregulation					898:909	upregulation	898:909	upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism	898:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	2	20	from	role	234:237	arg1	induction					258:266	the induction	254:266	the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells	254:338	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	4	21	theme	cells	639:643	arg1	proliferation					617:629	the proliferation	613:629	the proliferation of HeLa cells	613:643	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	1	22	located	found	154:158	arg2	Fucoidan					108:115	Fucoidan	108:115	Fucoidan	108:115	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	1	22	located	found	154:158	arg1	composition					173:183	cell-wall composition	163:183	cell-wall composition of brown algae	163:198	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	1	22	located	found	154:158	arg2	hetero-polysaccharide					131:151	a sulfated hetero-polysaccharide	120:151	a sulfated hetero-polysaccharide	120:151	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	7	23	theme	Fucoidan	1210:1217	arg1	treatment					1219:1227	Fucoidan treatment	1210:1227	Fucoidan treatment	1210:1227	Fucoidan treatment caused G1phase arrest by inducting CDKIs and inhibiting CDKs and Cyclins as well as apoptosis by activating the mitochondrial-dependent pathway in HeLa cells.
31295479	2	24	theme	stress-related	298:311	arg1	death					318:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Endoplasmic Reticulum (ER) stress-related cell death	271:322	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	7	25	from	pathway	1365:1371	arg1	cells					1381:1385	HeLa cells	1376:1385	HeLa cells	1376:1385	Fucoidan treatment caused G1phase arrest by inducting CDKIs and inhibiting CDKs and Cyclins as well as apoptosis by activating the mitochondrial-dependent pathway in HeLa cells.
31295479	2	26	theme	action	361:366	arg1	well-known					410:419	well-known	410:419	well-known	410:419	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	2	26	theme	action	361:366	arg1	mechanism					348:356	the mechanism	344:356	the mechanism of action of fucoidan in cervical HeLa cells	344:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	0	27	theme	In	0:1	arg1	induction					9:17	In vitro induction	0:17	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.	0:106	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	2	28	from	mechanism	348:356	arg1	cells					397:401	cervical HeLa cells	383:401	cervical HeLa cells	383:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	3	29	theme	G1	545:546	arg1	arrest					554:559	G1 phase arrest	545:559	G1 phase arrest	545:559	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	1	30	theme	brown	188:192	arg1	algae					194:198	brown algae	188:198	brown algae	188:198	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	5	31	theme	proteins	980:987	arg1	buildup					959:965	a buildup	957:965	a buildup of malfolded proteins	957:987	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	7	32	theme	HeLa	1376:1379	arg1	cells					1381:1385	HeLa cells	1376:1385	HeLa cells	1376:1385	Fucoidan treatment caused G1phase arrest by inducting CDKIs and inhibiting CDKs and Cyclins as well as apoptosis by activating the mitochondrial-dependent pathway in HeLa cells.
31295479	3	33	theme	cell	530:533	arg1	death					535:539	ER-stress-related cell death	512:539	ER-stress-related cell death	512:539	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	1	34	theme	algae	194:198	arg1	composition					173:183	cell-wall composition	163:183	cell-wall composition of brown algae	163:198	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	5	35	theme	HeLa	794:797	arg1	cells					799:803	HeLa cells	794:803	HeLa cells	794:803	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	36	theme	CHOP	925:928	arg1	Bip/GRP78					914:922	Bip/GRP78	914:922	Bip/GRP78	914:922	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	36	theme	CHOP	925:928	arg1	expression					930:939	CHOP expression	925:939	CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism	925:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	37	theme	sensors	878:884	arg1	phosphorylation					849:863	phosphorylation	849:863	phosphorylation of ER stress sensors	849:884	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	2	38	theme	HeLa	392:395	arg1	cells					397:401	cervical HeLa cells	383:401	cervical HeLa cells	383:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	0	39	theme	reticulum	34:42	arg1	stress					44:49	endoplasmic reticulum stress	22:49	endoplasmic reticulum stress	22:49	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	4	40	dep	lines	700:704	arg1	HaCaT					707:711	HaCaT	707:711	HaCaT: Keratinocytes and HEK-293: embryonic kidney	707:756	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	4	41	theme	HeLa	634:637	arg1	cells					639:643	HeLa cells	634:643	HeLa cells	634:643	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	5	42	dep	induced	841:847	arg1	followed					886:893	followed	886:893	followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism	886:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	2	43	theme	cervical	383:390	arg1	cells					397:401	cervical HeLa cells	383:401	cervical HeLa cells	383:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	0	44	theme	endoplasmic	22:32	arg1	stress					44:49	endoplasmic reticulum stress	22:49	endoplasmic reticulum stress	22:49	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	2	45	from	action	361:366	arg1	cells					397:401	cervical HeLa cells	383:401	cervical HeLa cells	383:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	7	46	theme	G1phase	1236:1242	arg1	arrest					1244:1249	G1phase arrest	1236:1249	G1phase arrest by inducting CDKIs and inhibiting CDKs	1236:1288	Fucoidan treatment caused G1phase arrest by inducting CDKIs and inhibiting CDKs and Cyclins as well as apoptosis by activating the mitochondrial-dependent pathway in HeLa cells.
31295479	3	47	theme	phase	548:552	arg1	arrest					554:559	G1 phase arrest	545:559	G1 phase arrest	545:559	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	2	48	theme	cancer	327:332	arg1	cells					334:338	cancer cells	327:338	cancer cells	327:338	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	0	49	theme	stress	44:49	arg1	induction					9:17	In vitro induction	0:17	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.	0:106	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	2	50	theme	fucoidan	371:378	arg1	action					361:366	action	361:366	action of fucoidan in cervical HeLa cells	361:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	8	51	theme	phase	1478:1482	arg1	arrest					1484:1489	G1 phase arrest	1475:1489	G1 phase arrest	1475:1489	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	5	52	theme	unfolded	1018:1025	arg1	UPR					1045:1047	UPR	1045:1047	UPR	1045:1047	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	52	theme	unfolded	1018:1025	arg1	response					1035:1042	unfolded protein response	1018:1042	unfolded protein response (UPR) mechanism	1018:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	4	53	theme	cell	695:698	arg1	lines					700:704	investigated normal cell lines	675:704	investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney)	675:757	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	8	54	theme	cells	1432:1436	arg1	proliferation					1438:1450	HeLa cells proliferation	1427:1450	HeLa cells proliferation	1427:1450	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	8	55	theme	G1	1475:1476	arg1	arrest					1484:1489	G1 phase arrest	1475:1489	G1 phase arrest	1475:1489	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	2	56	theme	Recent	201:206	arg1	studies					208:214	Recent studies	201:214	Recent studies	201:214	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	6	57	theme	stress-induced	1184:1197	arg1	apoptosis					1199:1207	the ER stress-induced apoptosis	1177:1207	the ER stress-induced apoptosis	1177:1207	In addition, intracellular calcium levels were elevated following the treatment suggesting that this contributed to the ER stress-induced apoptosis.
31295479	4	58	theme	treatment	659:667	arg1	48 h					651:654	48 h	651:654	48 h of treatment	651:667	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	2	59	theme	death	318:322	arg1	induction					258:266	the induction	254:266	the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells	254:338	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	2	60	from	induction	258:266	arg1	cells					334:338	cancer cells	327:338	cancer cells	327:338	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	6	61	theme	intracellular	1074:1086	arg1	levels					1096:1101	intracellular calcium levels	1074:1101	intracellular calcium levels	1074:1101	In addition, intracellular calcium levels were elevated following the treatment suggesting that this contributed to the ER stress-induced apoptosis.
31295479	5	62	theme	protein	1027:1033	arg1	UPR					1045:1047	UPR	1045:1047	UPR	1045:1047	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	62	theme	protein	1027:1033	arg1	response					1035:1042	unfolded protein response	1018:1042	unfolded protein response (UPR) mechanism	1018:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	4	63	theme	embryonic	741:749	arg1	kidney					751:756	embryonic kidney	741:756	HaCaT: Keratinocytes and HEK-293: embryonic kidney	707:756	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
31295479	8	64	theme	ER	1492:1493	arg1	stress					1495:1500	ER stress	1492:1500	ER stress	1492:1500	We demonstrated that Fucoidan inhibits HeLa cells proliferation by inducing apoptosis, G1 phase arrest, ER stress, and mitochondrial-mediated apoptosis.
31295479	1	65	theme	sulfated	122:129	arg1	Fucoidan					108:115	Fucoidan	108:115	Fucoidan	108:115	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	1	65	theme	sulfated	122:129	arg1	hetero-polysaccharide					131:151	a sulfated hetero-polysaccharide	120:151	a sulfated hetero-polysaccharide	120:151	Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae.
31295479	3	66	theme	study	442:446	arg1	purpose					426:432	The purpose	422:432	The purpose of this study	422:446	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	0	67	theme	cervical	60:67	arg1	cells					89:93	human cervical adenocarcinoma HeLa cells	54:93	human cervical adenocarcinoma HeLa cells	54:93	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	5	68	theme	response	1035:1042	arg1	mechanism					1050:1058	unfolded protein response (UPR) mechanism	1018:1058	unfolded protein response (UPR) mechanism	1018:1058	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	2	69	theme	fucoidan	242:249	arg1	role					234:237	the role	230:237	the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells	230:338	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	5	70	theme	cells	799:803	arg1	exposure					782:789	The exposure	778:789	The exposure of HeLa cells to these concentrations of fucoidan	778:839	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	71	theme	ER	868:869	arg1	sensors					878:884	ER stress sensors	868:884	ER stress sensors	868:884	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	5	72	theme	fucoidan	832:839	arg1	concentrations					814:827	these concentrations	808:827	these concentrations of fucoidan	808:839	The exposure of HeLa cells to these concentrations of fucoidan induced phosphorylation of ER stress sensors followed by upregulation of Bip/GRP78, CHOP expression which triggered a buildup of malfolded proteins in ER, therefore, initiating unfolded protein response (UPR) mechanism.
31295479	0	73	theme	human	54:58	arg1	cells					89:93	human cervical adenocarcinoma HeLa cells	54:93	human cervical adenocarcinoma HeLa cells	54:93	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	7	74	theme	mitochondrial-dependent	1341:1363	arg1	pathway					1365:1371	the mitochondrial-dependent pathway	1337:1371	the mitochondrial-dependent pathway in HeLa cells	1337:1385	Fucoidan treatment caused G1phase arrest by inducting CDKIs and inhibiting CDKs and Cyclins as well as apoptosis by activating the mitochondrial-dependent pathway in HeLa cells.
31295479	0	75	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
31295479	2	76	from	cells	397:401	arg1	well-known					410:419	well-known	410:419	well-known	410:419	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	2	76	from	cells	397:401	arg1	mechanism					348:356	the mechanism	344:356	the mechanism of action of fucoidan in cervical HeLa cells	344:401	Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known.
31295479	3	77	theme	HeLa	487:490	arg1	cells					492:496	HeLa cells	487:496	HeLa cells death	487:502	The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest.
31295479	4	78	theme	fucoidan	594:601	arg1	concentrations					576:589	200-600 μg/ml concentrations	562:589	200-600 μg/ml concentrations of fucoidan	562:601	200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT: Keratinocytes and HEK-293: embryonic kidney) were not affected.
29684453	7	0	theme	antimicrobial	1519:1531	arg1	agents					1533:1538	antimicrobial agents	1519:1538	antimicrobial agents	1519:1538	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	4	1	theme	antimicrobial	814:826	arg1	activities					828:837	better antimicrobial activities	807:837	better antimicrobial activities	807:837	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	7	2	theme	functionalized	1416:1429	arg1	moieties					1431:1438	functionalized moieties	1416:1438	functionalized moieties	1416:1438	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	0	3	theme	antimicrobial	88:100	arg1	agents					102:107	antimicrobial agents	88:107	antimicrobial agents	88:107	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	7	4	theme	promising	1495:1503	arg1	templates					1505:1513	promising templates	1495:1513	promising templates for antimicrobial agents	1495:1538	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	2	5	theme	MWCNT	554:558	arg1	biocomposites					561:573	Two multi-walled carbon nanotube (MWCNT) biocomposites	520:573	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4	520:595	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4 were also prepared.
29684453	6	6	theme	antimicrobial	1277:1289	arg1	activities					1291:1300	comparable or even better antimicrobial activities	1251:1300	comparable or even better antimicrobial activities	1251:1300	The MWCNT composites displayed comparable or even better antimicrobial activities than the reference bactericides or fungicides.
29684453	4	7	theme	zone	1041:1044	arg1	diameters					1046:1054	their higher inhibition zone diameters	1017:1054	their higher inhibition zone diameters using the agar well diffusion technique	1017:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	5	8	theme	Gram-negative	1196:1208	arg1	bacteria					1210:1217	Gram-negative bacteria	1196:1217	Gram-negative bacteria	1196:1217	These derivatives and MWCNT composites are more potent against Gram-positive bacteria than against Gram-negative bacteria.
29684453	7	9	with	combination	1399:1409	arg1	MWCNTs					1444:1449	MWCNTs	1444:1449	MWCNTs in one system	1444:1463	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	7	9	with	combination	1399:1409	arg1	moieties					1431:1438	functionalized moieties	1416:1438	functionalized moieties	1416:1438	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	3	10	theme	prepared	638:645	arg1	derivatives					647:657	the prepared derivatives	634:657	the prepared derivatives	634:657	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	0	11	link	cross-linked	21:32	arg1	chitosan					34:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	4	12	theme	inhibition	1030:1039	arg1	diameters					1046:1054	their higher inhibition zone diameters	1017:1054	their higher inhibition zone diameters using the agar well diffusion technique	1017:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	1	13	theme	amino	273:277	arg1	Derivative					287:296	Derivative 1	287:298	Derivative 1	287:298	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	1	13	theme	amino	273:277	arg1	groups					279:284	its amino groups	269:284	its amino groups (Derivative 1)	269:299	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	2	14	theme	nanotube	544:551	arg1	biocomposites					561:573	Two multi-walled carbon nanotube (MWCNT) biocomposites	520:573	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4	520:595	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4 were also prepared.
29684453	4	15	dep	faecalis	882:889	arg1	judged					1007:1012	judged	1007:1012	judged by their higher inhibition zone diameters using the agar well diffusion technique	1007:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	4	16	theme	MWCNT	783:787	arg1	composites					789:798	MWCNT composites	783:798	MWCNT composites	783:798	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	7	17	from	moieties	1431:1438	arg1	system					1458:1463	one system	1454:1463	one system	1454:1463	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	3	18	theme	MWCNT	663:667	arg1	composites					669:678	MWCNT composites	663:678	MWCNT composites	663:678	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	1	19	dep	derivatives	144:154	arg1	1-4					156:158	1-4	156:158	1-4	156:158	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	1	20	dep	restore	458:464	arg1	benzaldehyde					442:453	benzaldehyde	442:453	benzaldehyde	442:453	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	7	21	theme	structural	1355:1364	arg1	modification					1366:1377	structural modification	1355:1377	structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system	1355:1463	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	7	21	theme	structural	1355:1364	arg1	way					1480:1482	a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications	1478:1596	a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications	1478:1596	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	0	22	theme	aminohydrazide	6:19	arg1	chitosan					34:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	3	23	theme	composites	669:678	arg1	structure					621:629	The structure	617:629	The structure of the prepared derivatives and MWCNT composites	617:678	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	1	24	from	groups	481:486	arg1	Derivative					505:514	Derivative 4	505:516	Derivative 4	505:516	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	1	24	from	groups	481:486	arg1	chitosan					495:502	the chitosan	491:502	the chitosan (Derivative 4)	491:517	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	0	25	theme	Novel	0:4	arg1	chitosan					34:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	6	26	theme	comparable	1251:1260	arg1	activities					1291:1300	comparable or even better antimicrobial activities	1251:1300	comparable or even better antimicrobial activities	1251:1300	The MWCNT composites displayed comparable or even better antimicrobial activities than the reference bactericides or fungicides.
29684453	1	27	with	reaction	312:319	arg1	Derivative					344:353	Derivative 2	344:355	Derivative 2	344:355	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	1	27	with	reaction	312:319	arg1	epichlorohydrine					326:341	epichlorohydrine	326:341	epichlorohydrine (Derivative 2)	326:356	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	6	28	theme	better	1270:1275	arg1	activities					1291:1300	comparable or even better antimicrobial activities	1251:1300	comparable or even better antimicrobial activities	1251:1300	The MWCNT composites displayed comparable or even better antimicrobial activities than the reference bactericides or fungicides.
29684453	4	29	theme	diffusion	1076:1084	arg1	technique					1086:1094	the agar well diffusion technique	1062:1094	the agar well diffusion technique	1062:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	4	30	theme	better	807:812	arg1	activities					828:837	better antimicrobial activities	807:837	better antimicrobial activities	807:837	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	5	31	theme	Gram-positive	1160:1172	arg1	bacteria					1174:1181	Gram-positive bacteria	1160:1181	Gram-positive bacteria	1160:1181	These derivatives and MWCNT composites are more potent against Gram-positive bacteria than against Gram-negative bacteria.
29684453	4	32	theme	well	1071:1074	arg1	technique					1086:1094	the agar well diffusion technique	1062:1094	the agar well diffusion technique	1062:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	1	33	theme	reactions	211:219	arg1	series					196:201	a series	194:201	a series of four reactions	194:219	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	0	34	theme	cross-linked	21:32	arg1	chitosan					34:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan	0:41	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	1	35	theme	modified	126:133	arg1	derivatives					144:154	Four chemically modified chitosan derivatives 1-4	110:158	Four chemically modified chitosan derivatives 1-4	110:158	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	3	36	theme	elemental	701:709	arg1	analyses					711:718	elemental analyses	701:718	elemental analyses	701:718	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	3	36	theme	elemental	701:709	arg1	TEM					740:742	TEM	740:742	TEM	740:742	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	3	36	theme	elemental	701:709	arg1	SEM					732:734	SEM	732:734	SEM	732:734	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	3	36	theme	elemental	701:709	arg1	XRD					727:729	XRD	727:729	XRD	727:729	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	3	36	theme	elemental	701:709	arg1	FTIR					721:724	FTIR	721:724	FTIR	721:724	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	7	37	theme	medical	1577:1583	arg1	applications					1585:1596	medical applications	1577:1596	medical applications	1577:1596	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	4	38	theme	modified	749:756	arg1	derivatives					767:777	chitosan derivatives	758:777	chitosan derivatives	758:777	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	1	39	with	reaction	368:375	arg1	Derivative					402:411	Derivative 3	402:413	Derivative 3	402:413	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	1	39	with	reaction	368:375	arg1	aminobenzhydrazide					382:399	aminobenzhydrazide	382:399	aminobenzhydrazide (Derivative 3)	382:414	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	4	40	theme	Cryptococcus	957:968	arg1	neoformans					970:979	Cryptococcus neoformans	957:979	Cryptococcus neoformans	957:979	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	7	41	theme	appropriate	1550:1560	arg1	candidates					1562:1571	appropriate candidates	1550:1571	appropriate candidates for medical applications	1550:1596	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	0	42	theme	carbon	68:73	arg1	nanotubes					75:83	multi-walled carbon nanotubes	55:83	multi-walled carbon nanotubes as antimicrobial agents	55:107	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	7	43	dep	way	1480:1482	arg1	candidates					1562:1571	appropriate candidates	1550:1571	appropriate candidates for medical applications	1550:1596	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	7	43	dep	way	1480:1482	arg1	achieve					1487:1493	achieve	1487:1493	to achieve promising templates for antimicrobial agents	1484:1538	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	2	44	theme	carbon	537:542	arg1	biocomposites					561:573	Two multi-walled carbon nanotube (MWCNT) biocomposites	520:573	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4	520:595	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4 were also prepared.
29684453	6	45	theme	MWCNT	1224:1228	arg1	composites					1230:1239	The MWCNT composites	1220:1239	The MWCNT composites	1220:1239	The MWCNT composites displayed comparable or even better antimicrobial activities than the reference bactericides or fungicides.
29684453	1	46	theme	chitosan	135:142	arg1	derivatives					144:154	Four chemically modified chitosan derivatives 1-4	110:158	Four chemically modified chitosan derivatives 1-4	110:158	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	0	47	theme	multi-walled	55:66	arg1	nanotubes					75:83	multi-walled carbon nanotubes	55:83	multi-walled carbon nanotubes as antimicrobial agents	55:107	Novel aminohydrazide cross-linked chitosan filled with multi-walled carbon nanotubes as antimicrobial agents.
29684453	2	48	theme	multi-walled	524:535	arg1	biocomposites					561:573	Two multi-walled carbon nanotube (MWCNT) biocomposites	520:573	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4	520:595	Two multi-walled carbon nanotube (MWCNT) biocomposites based on Derivative 4 were also prepared.
29684453	4	49	theme	chitosan	758:765	arg1	derivatives					767:777	chitosan derivatives	758:777	chitosan derivatives	758:777	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	1	50	theme	free	470:473	arg1	groups					481:486	the free amino groups	466:486	the free amino groups on the chitosan (Derivative 4)	466:517	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	7	51	theme	chitosan	1382:1389	arg1	modification					1366:1377	structural modification	1355:1377	structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system	1355:1463	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	7	51	theme	chitosan	1382:1389	arg1	way					1480:1482	a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications	1478:1596	a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications	1478:1596	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	1	52	theme	amino	475:479	arg1	groups					481:486	the free amino groups	466:486	the free amino groups on the chitosan (Derivative 4)	466:517	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	7	53	from	MWCNTs	1444:1449	arg1	system					1458:1463	one system	1454:1463	one system	1454:1463	Thus, structural modification of chitosan through combination with functionalized moieties and MWCNTs in one system was taken as a way to achieve promising templates for antimicrobial agents and to be appropriate candidates for medical applications.
29684453	6	54	theme	reference	1311:1319	arg1	bactericides					1321:1332	the reference bactericides	1307:1332	the reference bactericides	1307:1332	The MWCNT composites displayed comparable or even better antimicrobial activities than the reference bactericides or fungicides.
29684453	4	55	theme	agar	1066:1069	arg1	technique					1086:1094	the agar well diffusion technique	1062:1094	the agar well diffusion technique	1062:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	5	56	theme	MWCNT	1119:1123	arg1	composites					1125:1134	MWCNT composites	1119:1134	MWCNT composites	1119:1134	These derivatives and MWCNT composites are more potent against Gram-positive bacteria than against Gram-negative bacteria.
29684453	4	57	dep	derivatives	767:777	arg1	The					745:747	The	745:747	The	745:747	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	4	58	theme	Candida	985:991	arg1	tropicalis					993:1002	Candida tropicalis	985:1002	Candida tropicalis	985:1002	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
29684453	3	59	theme	derivatives	647:657	arg1	structure					621:629	The structure	617:629	The structure of the prepared derivatives and MWCNT composites	617:678	The structure of the prepared derivatives and MWCNT composites was elucidated using elemental analyses, FTIR, XRD, SEM and TEM.
29684453	1	60	with	reaction	231:238	arg1	benzaldehyde					245:256	benzaldehyde	245:256	benzaldehyde	245:256	Four chemically modified chitosan derivatives 1-4 were designed and synthesized via a series of four reactions; first by reaction with benzaldehyde to protect its amino groups (Derivative 1), second by reaction with epichlorohydrine (Derivative 2), third by reaction with aminobenzhydrazide (Derivative 3), and forth by removing of benzaldehyde to restore the free amino groups on the chitosan (Derivative 4).
29684453	4	61	theme	higher	1023:1028	arg1	diameters					1046:1054	their higher inhibition zone diameters	1017:1054	their higher inhibition zone diameters using the agar well diffusion technique	1017:1094	The modified chitosan derivatives and MWCNT composites showed better antimicrobial activities than that of chitosan against Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger, Cryptococcus neoformans and Candida tropicalis as judged by their higher inhibition zone diameters using the agar well diffusion technique.
30284575	12	0	from	changes	1812:1818	arg1	amount					1835:1840	the protein amount	1823:1840	the protein amount	1823:1840	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	12	0	from	changes	1812:1818	arg1	source					1846:1851	source	1846:1851	source	1846:1851	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	6	1	theme	muscle	980:985	arg1	activities					987:996	The jaw muscle activities	972:996	The jaw muscle activities	972:996	The jaw muscle activities were highly dependent on the type of yogurt.
30284575	6	1	theme	muscle	980:985	arg1	dependent					1010:1018	dependent	1010:1018	dependent	1010:1018	The jaw muscle activities were highly dependent on the type of yogurt.
30284575	3	2	theme	microgels-added	625:639	arg1	WPM					642:644	WPM	642:644	WPM	642:644	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	2	theme	microgels-added	625:639	arg1	protein					617:623	whey protein microgels-added	612:639	whey protein microgels-added (WPM)	612:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	1	3	theme	improved	246:253	arg1	capacity					265:272	improved satiating capacity	246:272	improved satiating capacity which is largely influenced by oral exposure	246:317	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	5	4	dep	determined	938:947	arg1	system					963:968	jaw tracking system	950:968	jaw tracking system	950:968	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	11	5	theme	effective	1664:1672	arg1	way					1674:1676	a more effective way	1657:1676	a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source	1657:1795	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	5	theme	effective	1664:1672	arg1	they					1645:1648	they	1645:1648	they	1645:1648	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	3	6	theme	control	523:529	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	6	theme	control	523:529	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	6	theme	control	523:529	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	12	7	theme	behavioral	1901:1910	arg1	properties					1938:1947	the behavioral, rheological, and sensory properties	1897:1947	the behavioral, rheological, and sensory properties of yogurt	1897:1957	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	11	8	theme	processing	1615:1624	arg1	pattern					1626:1632	the oral processing pattern	1606:1632	the oral processing pattern	1606:1632	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	3	9	theme	skimmed	542:548	arg1	milk					550:553	extra skimmed milk powder-added	536:566	extra skimmed milk powder-added (MP)	536:571	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	9	theme	skimmed	542:548	arg1	MP					569:570	MP	569:570	MP	569:570	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	5	10	theme	temporalis	771:780	arg1	Activities					738:747	Activities	738:747	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample	738:849	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	10	11	theme	behavioral	1394:1403	arg1	results					1405:1411	These behavioral results	1388:1411	These behavioral results	1388:1411	These behavioral results, which clearly differentiate the samples, are discussed in connection to the rheological and sensory properties of the yogurts.
30284575	1	12	theme	food	193:196	arg1	composition					152:162	composition	152:162	composition	152:162	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	1	12	theme	food	193:196	arg1	properties					179:188	mechanical properties	168:188	mechanical properties	168:188	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	8	13	theme	milk	1283:1286	arg1	protein					1288:1294	milk protein	1283:1294	milk protein	1283:1294	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	12	14	theme	lesser	1882:1887	arg1	extent					1889:1894	a lesser extent	1880:1894	a lesser extent	1880:1894	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	11	15	theme	subtle	1750:1755	arg1	changes					1757:1763	more subtle changes	1745:1763	more subtle changes in the protein amount or source	1745:1795	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	1	16	theme	useful	207:212	arg1	information					214:224	useful information	207:224	useful information to design food	207:239	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	3	17	theme	powder-added	555:566	arg1	milk					550:553	extra skimmed milk powder-added	536:566	extra skimmed milk powder-added (MP)	536:571	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	17	theme	powder-added	555:566	arg1	MP					569:570	MP	569:570	MP	569:570	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	5	18	theme	oral	820:823	arg1	processing					825:834	oral processing	820:834	oral processing of each sample	820:849	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	5	19	theme	jaw	950:952	arg1	system					963:968	jaw tracking system	950:968	jaw tracking system	950:968	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	11	20	theme	protein	1772:1778	arg1	amount					1780:1785	the protein amount	1768:1785	the protein amount	1768:1785	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	12	21	theme	protein	1827:1833	arg1	amount					1835:1840	the protein amount	1823:1840	the protein amount	1823:1840	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	7	22	theme	processing	1097:1106	arg1	time					1108:1111	the oral processing time	1088:1111	the oral processing time	1088:1111	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	5	23	theme	anterior	786:793	arg1	muscles					805:811	masseter, anterior temporalis and anterior digastric muscles	752:811	muscles	805:811	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	1	24	dep	Understanding	77:89	arg1	altered					114:120	altered	114:120	is altered in response to changes in the composition and mechanical properties of food	111:196	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	5	25	theme	muscles	805:811	arg1	Activities					738:747	Activities	738:747	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample	738:849	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	3	26	theme	protein	579:585	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	26	theme	protein	579:585	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	26	theme	protein	579:585	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	8	27	theme	similar	1188:1194	arg1	values					1206:1211	similar but lower values	1188:1211	similar but lower values of oral processing	1188:1230	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	11	28	theme	apple	1573:1577	arg1	cubes					1579:1583	apple cubes	1573:1583	apple cubes	1573:1583	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	9	29	theme	weaker	1359:1364	arg1	material					1378:1385	a weaker, more fluid material	1357:1385	a weaker, more fluid material	1357:1385	The lowest values were found for WPI, C and WPM, indicating a weaker, more fluid material.
30284575	8	30	theme	lower	1200:1204	arg1	values					1206:1211	similar but lower values	1188:1211	similar but lower values of oral processing	1188:1230	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	11	31	theme	processing	1701:1710	arg1	time					1712:1715	the oral processing time	1692:1715	the oral processing time (time exposure)	1692:1731	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	31	theme	processing	1701:1710	arg1	exposure					1723:1730	time exposure	1718:1730	time exposure	1718:1730	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	0	32	theme	oral	62:65	arg1	activity					67:74	oral activity	62:74	oral activity	62:74	Impact of composition and texture of protein-added yogurts on oral activity.
30284575	7	33	theme	cubes	1061:1065	arg1	Addition					1043:1050	Addition	1043:1050	Addition of apple cubes (MPF)	1043:1071	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	11	34	dep	alters	1599:1604	arg1	way					1674:1676	a more effective way	1657:1676	a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source	1657:1795	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	34	dep	alters	1599:1604	arg1	they					1645:1648	they	1645:1648	they	1645:1648	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	5	35	theme	sample	844:849	arg1	processing					825:834	oral processing	820:834	oral processing of each sample	820:849	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	8	36	theme	processing	1221:1230	arg1	values					1206:1211	similar but lower values	1188:1211	similar but lower values of oral processing	1188:1230	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	10	37	theme	rheological	1490:1500	arg1	properties					1514:1523	the rheological and sensory properties	1486:1523	the rheological and sensory properties of the yogurts	1486:1538	These behavioral results, which clearly differentiate the samples, are discussed in connection to the rheological and sensory properties of the yogurts.
30284575	0	38	theme	composition	10:20	arg1	Impact					0:5	Impact	0:5	Impact of composition and texture of protein-added	0:49	Impact of composition and texture of protein-added yogurts on oral activity.
30284575	7	39	theme	samples	1158:1164	arg1	number					1114:1119	number	1114:1119	number of chews	1114:1128	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	7	39	theme	samples	1158:1164	arg1	time					1108:1111	the oral processing time	1088:1111	the oral processing time	1088:1111	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	7	39	theme	samples	1158:1164	arg1	activity					1142:1149	muscle activity	1135:1149	muscle activity of all samples	1135:1164	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	5	40	from	amplitudes	902:911	arg1	dimensions					922:931	three dimensions	916:931	three dimensions	916:931	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	2	41	theme	perception	400:409	arg1	physiology					378:387	the physiology	374:387	the physiology of texture perception	374:409	In turn, this information deepens the knowledge about the physiology of texture perception.
30284575	3	42	theme	different	445:453	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	42	theme	different	445:453	arg1	amounts					455:461	different amounts	445:461	different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	445:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	42	theme	different	445:453	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	42	theme	different	445:453	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	0	43	theme	texture	26:32	arg1	Impact					0:5	Impact	0:5	Impact of composition and texture of protein-added	0:49	Impact of composition and texture of protein-added yogurts on oral activity.
30284575	1	44	dep	composition	152:162	arg1	the					148:150	the	148:150	the	148:150	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	3	45	theme	sources	486:492	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	45	theme	sources	486:492	arg1	amounts					455:461	different amounts	445:461	different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	445:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	45	theme	sources	486:492	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	45	theme	sources	486:492	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	0	46	theme	protein-added	37:49	arg1	Impact					0:5	Impact	0:5	Impact of composition and texture of protein-added	0:49	Impact of composition and texture of protein-added yogurts on oral activity.
30284575	7	47	theme	muscle	1135:1140	arg1	activity					1142:1149	muscle activity	1135:1149	muscle activity of all samples	1135:1164	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	3	48	theme	protein	617:623	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	48	theme	protein	617:623	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	48	theme	protein	617:623	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	8	49	theme	reinforced	1261:1270	arg1	network					1272:1278	their reinforced network	1255:1278	their reinforced network of milk protein	1255:1294	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	12	50	theme	sensory	1930:1936	arg1	properties					1938:1947	the behavioral, rheological, and sensory properties	1897:1947	the behavioral, rheological, and sensory properties of yogurt	1897:1957	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	10	51	theme	sensory	1506:1512	arg1	properties					1514:1523	the rheological and sensory properties	1486:1523	the rheological and sensory properties of the yogurts	1486:1538	These behavioral results, which clearly differentiate the samples, are discussed in connection to the rheological and sensory properties of the yogurts.
30284575	5	52	theme	jaw	889:891	arg1	amplitudes					902:911	jaw movement amplitudes	889:911	jaw movement amplitudes in three dimensions	889:931	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	2	53	theme	texture	392:398	arg1	perception					400:409	texture perception	392:409	texture perception	392:409	In turn, this information deepens the knowledge about the physiology of texture perception.
30284575	4	54	theme	fresh	713:717	arg1	cubes					725:729	fresh apple cubes	713:729	fresh apple cubes (MPF)	713:735	In addition, MP was also added with maltodextrin (MPMD) and with fresh apple cubes (MPF).
30284575	4	54	theme	fresh	713:717	arg1	MPF					732:734	MPF	732:734	MPF	732:734	In addition, MP was also added with maltodextrin (MPMD) and with fresh apple cubes (MPF).
30284575	3	55	theme	apple	510:514	arg1	cubes					516:520	apple cubes	510:520	apple cubes	510:520	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	1	56	theme	design	229:234	arg1	food					236:239	design food	229:239	design food	229:239	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	3	57	theme	addition	498:505	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	57	theme	addition	498:505	arg1	amounts					455:461	different amounts	445:461	different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	445:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	57	theme	addition	498:505	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	57	theme	addition	498:505	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	6	58	theme	jaw	976:978	arg1	activities					987:996	The jaw muscle activities	972:996	The jaw muscle activities	972:996	The jaw muscle activities were highly dependent on the type of yogurt.
30284575	6	58	theme	jaw	976:978	arg1	dependent					1010:1018	dependent	1010:1018	dependent	1010:1018	The jaw muscle activities were highly dependent on the type of yogurt.
30284575	12	59	theme	yogurt	1952:1957	arg1	properties					1938:1947	the behavioral, rheological, and sensory properties	1897:1947	the behavioral, rheological, and sensory properties of yogurt	1897:1957	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	1	60	theme	satiating	255:263	arg1	capacity					265:272	improved satiating capacity	246:272	improved satiating capacity which is largely influenced by oral exposure	246:317	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	11	61	from	changes	1757:1763	arg1	source					1790:1795	source	1790:1795	source	1790:1795	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	61	from	changes	1757:1763	arg1	amount					1780:1785	the protein amount	1768:1785	the protein amount	1768:1785	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	1	62	theme	mechanical	168:177	arg1	properties					179:188	mechanical properties	168:188	mechanical properties	168:188	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	5	63	theme	masseter	752:759	arg1	temporalis					771:780	masseter, anterior temporalis and anterior digastric muscles	752:811	temporalis	771:780	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	3	64	theme	extra	536:540	arg1	milk					550:553	extra skimmed milk powder-added	536:566	extra skimmed milk powder-added (MP)	536:571	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	64	theme	extra	536:540	arg1	MP					569:570	MP	569:570	MP	569:570	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	5	65	theme	anterior	762:769	arg1	temporalis					771:780	masseter, anterior temporalis and anterior digastric muscles	752:811	temporalis	771:780	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	6	66	theme	yogurt	1035:1040	arg1	type					1027:1030	the type	1023:1030	the type of yogurt	1023:1040	The jaw muscle activities were highly dependent on the type of yogurt.
30284575	9	67	theme	lowest	1301:1306	arg1	values					1308:1313	The lowest values	1297:1313	The lowest values	1297:1313	The lowest values were found for WPI, C and WPM, indicating a weaker, more fluid material.
30284575	8	68	theme	protein	1288:1294	arg1	network					1272:1278	their reinforced network	1255:1278	their reinforced network of milk protein	1255:1294	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	11	69	theme	oral	1610:1613	arg1	pattern					1626:1632	the oral processing pattern	1606:1632	the oral processing pattern	1606:1632	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	3	70	theme	milk	550:553	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	70	theme	milk	550:553	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	70	theme	milk	550:553	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	11	71	dep	way	1674:1676	arg1	such					1635:1638	such	1635:1638	such	1635:1638	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	12	72	theme	rheological	1913:1923	arg1	properties					1938:1947	the behavioral, rheological, and sensory properties	1897:1947	the behavioral, rheological, and sensory properties of yogurt	1897:1957	Nevertheless, changes in the protein amount and source also affected, although to a lesser extent, the behavioral, rheological, and sensory properties of yogurt.
30284575	3	73	theme	whey	574:577	arg1	WPI					602:604	WPI	602:604	WPI	602:604	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	73	theme	whey	574:577	arg1	protein					579:585	whey protein isolate-added	574:599	whey protein isolate-added (WPI)	574:605	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	9	74	dep	weaker	1359:1364	arg1	fluid					1372:1376	fluid	1372:1376	fluid	1372:1376	The lowest values were found for WPI, C and WPM, indicating a weaker, more fluid material.
30284575	7	75	theme	oral	1092:1095	arg1	time					1108:1111	the oral processing time	1088:1111	the oral processing time	1088:1111	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	5	76	theme	tracking	954:961	arg1	system					963:968	jaw tracking system	950:968	jaw tracking system	950:968	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	5	77	dep	recorded	856:863	arg1	electromyography					866:881	electromyography	866:881	electromyography	866:881	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	5	78	theme	digastric	795:803	arg1	muscles					805:811	masseter, anterior temporalis and anterior digastric muscles	752:811	muscles	805:811	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	1	79	from	changes	137:143	arg1	composition					152:162	composition	152:162	composition	152:162	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	1	79	from	changes	137:143	arg1	properties					179:188	mechanical properties	168:188	mechanical properties	168:188	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	7	80	theme	apple	1055:1059	arg1	MPF					1068:1070	MPF	1068:1070	MPF	1068:1070	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	7	80	theme	apple	1055:1059	arg1	cubes					1061:1065	apple cubes	1055:1065	apple cubes (MPF)	1055:1071	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	11	81	theme	oral	1696:1699	arg1	time					1712:1715	the oral processing time	1692:1715	the oral processing time (time exposure)	1692:1731	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	81	theme	oral	1696:1699	arg1	exposure					1723:1730	time exposure	1718:1730	time exposure	1718:1730	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	1	82	theme	oral	95:98	arg1	processing					100:109	oral processing	95:109	oral processing	95:109	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	8	83	theme	oral	1216:1219	arg1	processing					1221:1230	oral processing	1216:1230	oral processing	1216:1230	MP and MPMD required similar but lower values of oral processing than MPF attributed to their reinforced network of milk protein.
30284575	3	84	theme	protein	466:472	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	84	theme	protein	466:472	arg1	amounts					455:461	different amounts	445:461	different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	445:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	84	theme	protein	466:472	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	84	theme	protein	466:472	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	85	theme	isolate-added	587:599	arg1	WPI					602:604	WPI	602:604	WPI	602:604	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	85	theme	isolate-added	587:599	arg1	protein					579:585	whey protein isolate-added	574:599	whey protein isolate-added (WPI)	574:605	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	4	86	theme	apple	719:723	arg1	cubes					725:729	fresh apple cubes	713:729	fresh apple cubes (MPF)	713:735	In addition, MP was also added with maltodextrin (MPMD) and with fresh apple cubes (MPF).
30284575	4	86	theme	apple	719:723	arg1	MPF					732:734	MPF	732:734	MPF	732:734	In addition, MP was also added with maltodextrin (MPMD) and with fresh apple cubes (MPF).
30284575	11	87	theme	time	1718:1721	arg1	time					1712:1715	the oral processing time	1692:1715	the oral processing time (time exposure)	1692:1731	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	11	87	theme	time	1718:1721	arg1	exposure					1723:1730	time exposure	1718:1730	time exposure	1718:1730	This study suggests that adding apple cubes significantly alters the oral processing pattern, such that they may be a more effective way of increasing the oral processing time (time exposure) compared to more subtle changes in the protein amount or source.
30284575	1	88	theme	oral	305:308	arg1	exposure					310:317	oral exposure	305:317	oral exposure	305:317	Understanding how oral processing is altered in response to changes in the composition and mechanical properties of food provides useful information to design food with improved satiating capacity which is largely influenced by oral exposure.
30284575	3	89	theme	whey	612:615	arg1	WPM					642:644	WPM	642:644	WPM	642:644	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	89	theme	whey	612:615	arg1	protein					617:623	whey protein microgels-added	612:639	whey protein microgels-added (WPM)	612:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	7	90	theme	chews	1124:1128	arg1	number					1114:1119	number	1114:1119	number of chews	1114:1128	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	7	90	theme	chews	1124:1128	arg1	time					1108:1111	the oral processing time	1088:1111	the oral processing time	1088:1111	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	7	90	theme	chews	1124:1128	arg1	activity					1142:1149	muscle activity	1135:1149	muscle activity of all samples	1135:1164	Addition of apple cubes (MPF) almost doubled the oral processing time, number of chews, and muscle activity of all samples.
30284575	3	91	theme	protein	478:484	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	5	92	theme	movement	893:900	arg1	amplitudes					902:911	jaw movement amplitudes	889:911	jaw movement amplitudes in three dimensions	889:931	Activities of masseter, anterior temporalis and anterior digastric muscles during oral processing of each sample were recorded (electromyography), and jaw movement amplitudes in three dimensions were determined (jaw tracking system).
30284575	10	93	theme	yogurts	1532:1538	arg1	properties					1514:1523	the rheological and sensory properties	1486:1523	the rheological and sensory properties of the yogurts	1486:1538	These behavioral results, which clearly differentiate the samples, are discussed in connection to the rheological and sensory properties of the yogurts.
30284575	3	94	theme	cubes	516:520	arg1	sources					486:492	protein sources	478:492	protein sources	478:492	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	94	theme	cubes	516:520	arg1	addition					498:505	addition	498:505	addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM)	498:645	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
30284575	3	94	theme	cubes	516:520	arg1	protein					466:472	protein	466:472	protein	466:472	Six yogurts were formulated with different amounts of protein and protein sources and addition of apple cubes: control (C), extra skimmed milk powder-added (MP), whey protein isolate-added (WPI), and whey protein microgels-added (WPM).
31181638	0	0	theme	Fermented	62:70	arg1	Microbiome					82:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	2	1	theme	ex	450:451	arg1	system					471:476	an ex vivo fermentation system	447:476	an ex vivo fermentation system to simulate the colonic environment	447:512	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	7	2	theme	acidification	1070:1082	arg1	activity					1084:1091	acidification activity	1070:1091	acidification activity	1070:1091	HPDI and LPDI seemed to be similar to each other regarding composition and acidification activity, but different from the control and FOS.
31181638	1	3	theme	gastrointestinal	136:151	arg1	health					153:158	gastrointestinal health	136:158	gastrointestinal health	136:158	Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria.
31181638	10	4	theme	greater	1441:1447	arg1	effect					1449:1454	a greater effect	1439:1454	a greater effect than FOS	1439:1463	In conclusion, the prebiotic activity of fructans appears to depend on the degree of polymerization, with LPDI and especially HPDI having a greater effect than FOS.
31181638	7	5	dep	control	1117:1123	arg1	the					1113:1115	the	1113:1115	the	1113:1115	HPDI and LPDI seemed to be similar to each other regarding composition and acidification activity, but different from the control and FOS.
31181638	5	6	theme	fermentation	857:868	arg1	inoculation					830:840	inoculation	830:840	inoculation of the ex vivo fermentation	830:868	Alpha-diversity decreased upon inoculation of the ex vivo fermentation under all treatments.
31181638	3	7	theme	baseline	599:606	arg1	enterotype					608:617	the same baseline enterotype	590:617	the same baseline enterotype	590:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	0	8	theme	Gut	78:80	arg1	Microbiome					82:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	0	9	from	Effect	0:5	arg1	Microbiome					82:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	3	10	theme	pooled	546:551	arg1	feces					553:557	pooled feces	546:557	pooled feces from three healthy donors with the same baseline enterotype	546:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	6	11	theme	p	966:966	arg1	<					968:968	ANOSIM p < 0.005	959:974	ANOSIM p < 0.005	959:974	Composition changed significantly across both treatments and time (ANOSIM p < 0.005 for both factors).
31181638	2	12	theme	polymerization	304:317	arg1	degrees					293:299	increasing degrees	282:299	increasing degrees of polymerization	282:317	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	13	theme	fructans	268:275	arg1	effects					257:263	the potential prebiotic effects	233:263	the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins	233:366	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	6	14	theme	ANOSIM	959:964	arg1	<					968:968	ANOSIM p < 0.005	959:974	ANOSIM p < 0.005	959:974	Composition changed significantly across both treatments and time (ANOSIM p < 0.005 for both factors).
31181638	7	15	from	control	1117:1123	arg1	different					1098:1106	different	1098:1106	different	1098:1106	HPDI and LPDI seemed to be similar to each other regarding composition and acidification activity, but different from the control and FOS.
31181638	10	16	theme	polymerization	1386:1399	arg1	degree					1376:1381	the degree	1372:1381	the degree of polymerization	1372:1399	In conclusion, the prebiotic activity of fructans appears to depend on the degree of polymerization, with LPDI and especially HPDI having a greater effect than FOS.
31181638	8	17	theme	acidification	1196:1208	arg1	terms					1167:1171	terms	1167:1171	terms of composition but not acidification	1167:1208	FOS differed from the control in terms of composition but not acidification.
31181638	4	18	theme	fermentation	725:736	arg1	days					717:720	2, 7, and 14 days	704:720	2, 7, and 14 days of fermentation	704:736	Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment.
31181638	3	19	from	donors	578:583	arg1	feces					553:557	pooled feces	546:557	pooled feces from three healthy donors with the same baseline enterotype	546:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	8	20	theme	composition	1176:1186	arg1	terms					1167:1171	terms	1167:1171	terms of composition but not acidification	1167:1208	FOS differed from the control in terms of composition but not acidification.
31181638	10	21	theme	fructans	1342:1349	arg1	activity					1330:1337	the prebiotic activity	1316:1337	the prebiotic activity of fructans	1316:1349	In conclusion, the prebiotic activity of fructans appears to depend on the degree of polymerization, with LPDI and especially HPDI having a greater effect than FOS.
31181638	2	22	theme	low	375:377	arg1	degree					403:408	a low and high polymerization degree	373:408	a low and high polymerization degree (LPDI and HPDI, respectively)	373:438	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	7	23	from	FOS	1129:1131	arg1	different					1098:1106	different	1098:1106	different	1098:1106	HPDI and LPDI seemed to be similar to each other regarding composition and acidification activity, but different from the control and FOS.
31181638	0	24	theme	Ex	54:55	arg1	Microbiome					82:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	1	25	used	used	120:123	arg2	supplements					104:114	Prebiotic supplements	94:114	Prebiotic supplements	94:114	Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria.
31181638	4	26	from	Changes	620:626	arg1	composition					642:652	microbiota composition	631:652	microbiota composition	631:652	Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment.
31181638	1	27	theme	beneficial	175:184	arg1	bacteria					186:193	beneficial bacteria	175:193	beneficial bacteria	175:193	Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria.
31181638	4	28	theme	microbiota	631:640	arg1	composition					642:652	microbiota composition	631:652	microbiota composition	631:652	Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment.
31181638	0	29	theme	Degree	14:19	arg1	Effect					0:5	Effect	0:5	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.	0:92	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	10	30	theme	prebiotic	1320:1328	arg1	activity					1330:1337	the prebiotic activity	1316:1337	the prebiotic activity of fructans	1316:1349	In conclusion, the prebiotic activity of fructans appears to depend on the degree of polymerization, with LPDI and especially HPDI having a greater effect than FOS.
31181638	0	31	dep	Ex	54:55	arg1	Vivo					57:60	Vivo	57:60	Vivo	57:60	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	9	32	theme	Bonferroni	1279:1288	arg1	control					1270:1276	the control	1266:1276	the control (Bonferroni p < 0.05)	1266:1298	HDPI restored alpha-diversity on day 14 as compared to the control (Bonferroni p < 0.05).
31181638	9	32	theme	Bonferroni	1279:1288	arg1	<					1292:1292	Bonferroni p < 0.05	1279:1297	Bonferroni p < 0.05	1279:1297	HDPI restored alpha-diversity on day 14 as compared to the control (Bonferroni p < 0.05).
31181638	2	33	theme	increasing	282:291	arg1	degrees					293:299	increasing degrees	282:299	increasing degrees of polymerization	282:317	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	5	34	theme	ex	849:850	arg1	fermentation					857:868	the ex vivo fermentation	845:868	the ex vivo fermentation	845:868	Alpha-diversity decreased upon inoculation of the ex vivo fermentation under all treatments.
31181638	0	35	theme	Polymerization	24:37	arg1	Degree					14:19	the Degree	10:19	the Degree of Polymerization of Fructans	10:49	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	4	36	theme	metagenomic	675:685	arg1	sequencing					687:696	metagenomic sequencing	675:696	metagenomic sequencing	675:696	Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment.
31181638	2	37	theme	polymerization	388:401	arg1	degree					403:408	a low and high polymerization degree	373:408	a low and high polymerization degree (LPDI and HPDI, respectively)	373:438	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	38	theme	colonic	494:500	arg1	environment					502:512	the colonic environment	490:512	the colonic environment	490:512	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	3	39	theme	same	594:597	arg1	enterotype					608:617	the same baseline enterotype	590:617	the same baseline enterotype	590:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	2	40	theme	high	383:386	arg1	degree					403:408	a low and high polymerization degree	373:408	a low and high polymerization degree (LPDI and HPDI, respectively)	373:438	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	0	41	theme	Fructans	42:49	arg1	Polymerization					24:37	Polymerization	24:37	Polymerization of Fructans	24:49	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
31181638	2	42	dep	ex	450:451	arg1	vivo					453:456	vivo	453:456	vivo	453:456	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	43	with	fructooligosaccharides	327:348	arg1	degrees					293:299	increasing degrees	282:299	increasing degrees of polymerization	282:317	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	44	theme	study	212:216	arg1	aim					200:202	The aim	196:202	The aim of this study	196:216	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	1	45	theme	Prebiotic	94:102	arg1	supplements					104:114	Prebiotic supplements	94:114	Prebiotic supplements	94:114	Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria.
31181638	2	46	dep	degree	403:408	arg1	HPDI					420:423	HPDI	420:423	HPDI	420:423	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	46	dep	degree	403:408	arg1	LPDI					411:414	LPDI	411:414	LPDI	411:414	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	47	theme	fructooligosaccharides	327:348	arg1	effects					257:263	the potential prebiotic effects	233:263	the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins	233:366	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	4	48	theme	acid	743:746	arg1	production					748:757	acid production	743:757	acid production	743:757	Changes in microbiota composition were measured by 16S metagenomic sequencing after 2, 7, and 14 days of fermentation, and acid production was measured throughout the experiment.
31181638	2	49	theme	prebiotic	247:255	arg1	effects					257:263	the potential prebiotic effects	233:263	the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins	233:366	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	5	50	dep	ex	849:850	arg1	vivo					852:855	vivo	852:855	vivo	852:855	Alpha-diversity decreased upon inoculation of the ex vivo fermentation under all treatments.
31181638	2	51	with	inulins	360:366	arg1	degrees					293:299	increasing degrees	282:299	increasing degrees of polymerization	282:317	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	52	theme	potential	237:245	arg1	effects					257:263	the potential prebiotic effects	233:263	the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins	233:366	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	2	53	theme	fermentation	458:469	arg1	system					471:476	an ex vivo fermentation system	447:476	an ex vivo fermentation system to simulate the colonic environment	447:512	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	6	54	dep	time	953:956	arg1	<					968:968	ANOSIM p < 0.005	959:974	ANOSIM p < 0.005	959:974	Composition changed significantly across both treatments and time (ANOSIM p < 0.005 for both factors).
31181638	3	55	theme	healthy	570:576	arg1	donors					578:583	three healthy donors	564:583	three healthy donors with the same baseline enterotype	564:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	2	56	theme	inulins	360:366	arg1	effects					257:263	the potential prebiotic effects	233:263	the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins	233:366	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	9	57	theme	p	1290:1290	arg1	control					1270:1276	the control	1266:1276	the control (Bonferroni p < 0.05)	1266:1298	HDPI restored alpha-diversity on day 14 as compared to the control (Bonferroni p < 0.05).
31181638	9	57	theme	p	1290:1290	arg1	<					1292:1292	Bonferroni p < 0.05	1279:1297	Bonferroni p < 0.05	1279:1297	HDPI restored alpha-diversity on day 14 as compared to the control (Bonferroni p < 0.05).
31181638	3	58	with	donors	578:583	arg1	enterotype					608:617	the same baseline enterotype	590:617	the same baseline enterotype	590:617	The system was inoculated with pooled feces from three healthy donors with the same baseline enterotype.
31181638	2	59	with	fructans	268:275	arg1	degrees					293:299	increasing degrees	282:299	increasing degrees of polymerization	282:317	The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the colonic environment.
31181638	0	60	theme	Human	72:76	arg1	Microbiome					82:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Ex Vivo Fermented Human Gut Microbiome	54:91	Effect of the Degree of Polymerization of Fructans on Ex Vivo Fermented Human Gut Microbiome.
30964270	0	0	theme	Peptide	89:95	arg1	Antigen					97:103	an HIV Peptide Antigen	82:103	an HIV Peptide Antigen	82:103	Polysaccharide Nanoparticles Can Efficiently Modulate the Immune Response against an HIV Peptide Antigen.
30964270	2	1	theme	HIV	442:444	arg1	maturation					412:421	maturation	412:421	maturation	412:421	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	1	theme	HIV	442:444	arg1	infectivity					427:437	infectivity	427:437	infectivity	427:437	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	2	theme	protease	323:330	arg1	PCS1-12					348:354	PCS1-12	348:354	PCS1-12	348:354	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	2	theme	protease	323:330	arg1	sites					341:345	the 12 highly conserved protease cleavage sites	299:345	the 12 highly conserved protease cleavage sites (PCS1-12)	299:355	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	8	3	contain	containing	1334:1343	arg1	Nanoparticles					1320:1332	Nanoparticles	1320:1332	Nanoparticles containing PCS5 conjugated and poly(I:C)	1320:1373	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	8	3	contain	containing	1334:1343	arg2	poly					1365:1368	poly	1365:1368	poly(I:C)	1365:1373	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	8	3	contain	containing	1334:1343	arg2	C					1372:1372	I:C	1370:1372	I:C	1370:1372	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	8	3	contain	containing	1334:1343	arg2	PCS5					1345:1348	PCS5	1345:1348	PCS5 conjugated	1345:1359	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	0	4	theme	HIV	85:87	arg1	Antigen					97:103	an HIV Peptide Antigen	82:103	an HIV Peptide Antigen	82:103	Polysaccharide Nanoparticles Can Efficiently Modulate the Immune Response against an HIV Peptide Antigen.
30964270	2	5	theme	conserved	313:321	arg1	PCS1-12					348:354	PCS1-12	348:354	PCS1-12	348:354	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	5	theme	conserved	313:321	arg1	sites					341:345	the 12 highly conserved protease cleavage sites	299:345	the 12 highly conserved protease cleavage sites (PCS1-12)	299:355	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	4	6	theme	immune	863:868	arg1	response					870:877	the generated immune response	849:877	the generated immune response	849:877	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	5	7	dep	polymer	1074:1080	arg1	chitosan					1103:1110	chitosan	1103:1110	chitosan	1103:1110	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	7	dep	polymer	1074:1080	arg1	sulfate					1091:1097	dextran sulfate	1083:1097	dextran sulfate	1083:1097	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	9	8	theme	cell	1463:1466	arg1	activation					1468:1477	T cell activation	1461:1477	T cell activation	1461:1477	Interestingly, T cell activation presented different kinetics depending on the prototype.
30964270	4	9	theme	peptide	709:715	arg1	attachment					689:698	the covalent attachment	676:698	the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles	676:760	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	9	theme	peptide	709:715	arg1	coadministration					773:788	their coadministration	767:788	their coadministration with polyinosinic:polycytidylic acid (poly(I:C))	767:837	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	10	theme	generated	853:861	arg1	response					870:877	the generated immune response	849:877	the generated immune response	849:877	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	5	11	theme	I	1122:1122	arg1	poly					1117:1120	poly	1117:1120	poly(I:C)	1117:1125	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	11	theme	I	1122:1122	arg1	C					1124:1124	I:C	1122:1124	I:C	1122:1124	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	4	12	theme	PCS	705:707	arg1	peptide					709:715	a PCS peptide	703:715	a PCS peptide (PCS5)	703:722	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	12	theme	PCS	705:707	arg1	PCS5					718:721	PCS5	718:721	PCS5	718:721	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	3	13	theme	research	470:477	arg1	line					462:465	this line	457:465	this line of research	457:477	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	6	14	theme	control	1238:1244	arg1	Nanoparticles					1154:1166	Nanoparticles	1154:1166	Nanoparticles associating PCS5 by ionic interactions	1154:1205	Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation.
30964270	6	14	theme	control	1238:1244	arg1	formulation					1246:1256	the control formulation	1234:1256	the control formulation	1234:1256	Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation.
30964270	4	15	dep	acid	822:825	arg1	C					835:835	I:C	833:835	I:C	833:835	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	15	dep	acid	822:825	arg1	poly					828:831	poly	828:831	poly(I:C)	828:836	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	1	16	theme	major	168:172	arg1	challenge					181:189	a major health challenge	166:189	a major health challenge	166:189	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	1	16	theme	major	168:172	arg1	development					110:120	The development	106:120	The development of an effective HIV vaccine	106:148	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	10	17	theme	antigen	1634:1640	arg1	composition					1583:1593	the nanoparticle composition	1566:1593	the nanoparticle composition	1566:1593	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	10	17	theme	antigen	1634:1640	arg1	conjugation					1603:1613	the conjugation	1599:1613	the conjugation of the HIV peptide antigen	1599:1640	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	5	18	theme	dextran	1083:1089	arg1	sulfate					1091:1097	dextran sulfate	1083:1097	dextran sulfate	1083:1097	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	4	19	theme	polycytidylic	808:820	arg1	acid					822:825	polycytidylic acid	808:825	polyinosinic:polycytidylic acid (poly(I:C))	795:837	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	5	20	theme	charged	1066:1072	arg1	polymer					1074:1080	an oppositely charged polymer	1052:1080	an oppositely charged polymer (dextran sulfate and chitosan)	1052:1111	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	10	21	theme	important	1654:1662	arg1	role					1664:1667	an important role	1651:1667	an important role	1651:1667	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	1	22	theme	health	174:179	arg1	challenge					181:189	a major health challenge	166:189	a major health challenge	166:189	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	1	22	theme	health	174:179	arg1	development					110:120	The development	106:120	The development of an effective HIV vaccine	106:148	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	0	23	theme	Polysaccharide	0:13	arg1	Nanoparticles					15:27	Polysaccharide Nanoparticles	0:27	Polysaccharide Nanoparticles	0:27	Polysaccharide Nanoparticles Can Efficiently Modulate the Immune Response against an HIV Peptide Antigen.
30964270	1	24	theme	modest	246:251	arg1	efficacy					262:269	a modest clinical efficacy	244:269	a modest clinical efficacy	244:269	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	9	25	theme	different	1489:1497	arg1	kinetics					1499:1506	different kinetics	1489:1506	different kinetics	1489:1506	Interestingly, T cell activation presented different kinetics depending on the prototype.
30964270	3	26	theme	low	514:516	arg1	immunogenicity					518:531	low immunogenicity	514:531	low immunogenicity	514:531	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	4	27	dep	polyinosinic	795:806	arg1	acid					822:825	polycytidylic acid	808:825	polyinosinic:polycytidylic acid (poly(I:C))	795:837	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	1	28	theme	clinical	253:260	arg1	efficacy					262:269	a modest clinical efficacy	244:269	a modest clinical efficacy	244:269	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	8	29	theme	antigen-presenting	1420:1437	arg1	cells					1439:1443	antigen-presenting cells	1420:1443	antigen-presenting cells	1420:1443	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	3	30	theme	peptide	492:498	arg1	antigens					500:507	peptide antigens	492:507	peptide antigens	492:507	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	8	31	theme	I	1370:1370	arg1	C					1372:1372	I:C	1370:1372	I:C	1370:1372	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	8	31	theme	I	1370:1370	arg1	poly					1365:1368	poly	1365:1368	poly(I:C)	1365:1373	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	4	32	theme	covalent	680:687	arg1	attachment					689:698	the covalent attachment	676:698	the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles	676:760	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	10	33	theme	HIV	1622:1624	arg1	antigen					1634:1640	the HIV peptide antigen	1618:1640	the HIV peptide antigen	1618:1640	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	10	34	theme	peptide	1626:1632	arg1	antigen					1634:1640	the HIV peptide antigen	1618:1640	the HIV peptide antigen	1618:1640	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	1	35	theme	vaccine	142:148	arg1	challenge					181:189	a major health challenge	166:189	a major health challenge	166:189	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	1	35	theme	vaccine	142:148	arg1	development					110:120	The development	106:120	The development of an effective HIV vaccine	106:148	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	3	36	contain	have	509:512	arg1	antigens					500:507	peptide antigens	492:507	peptide antigens	492:507	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	3	36	contain	have	509:512	arg2	immunogenicity					518:531	low immunogenicity	514:531	low immunogenicity	514:531	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	7	37	theme	anti-PCS5	1298:1306	arg1	antibodies					1308:1317	high anti-PCS5 antibodies	1293:1317	high anti-PCS5 antibodies	1293:1317	In vivo, all nanosystems elicited high anti-PCS5 antibodies.
30964270	8	38	theme	cells	1439:1443	arg1	activation					1406:1415	the strongest activation	1392:1415	the strongest activation of antigen-presenting cells	1392:1443	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	6	39	theme	ionic	1188:1192	arg1	interactions					1194:1205	ionic interactions	1188:1205	ionic interactions	1188:1205	Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation.
30964270	3	40	theme	engineered	569:578	arg1	nanoparticles					580:592	engineered nanoparticles	569:592	engineered nanoparticles	569:592	To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation.
30964270	9	41	theme	T	1461:1461	arg1	activation					1468:1477	T cell activation	1461:1477	T cell activation	1461:1477	Interestingly, T cell activation presented different kinetics depending on the prototype.
30964270	4	42	attach	attachment	689:698	arg2	PCS5					718:721	PCS5	718:721	PCS5	718:721	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	42	attach	attachment	689:698	arg2	peptide					709:715	a PCS peptide	703:715	a PCS peptide (PCS5)	703:722	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	42	attach	attachment	689:698	arg1	nanoparticles					748:760	polysaccharide-based nanoparticles	727:760	polysaccharide-based nanoparticles	727:760	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	10	43	theme	responses	1711:1719	arg1	generation					1676:1685	the generation	1672:1685	the generation of humoral and cellular responses	1672:1719	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	5	44	theme	different	926:934	arg1	chitosan					953:960	chitosan	953:960	chitosan	953:960	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	44	theme	different	926:934	arg1	acid					977:980	hyaluronic acid	966:980	hyaluronic acid	966:980	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	44	theme	different	926:934	arg1	polysaccharides					936:950	two different polysaccharides	922:950	two different polysaccharides (chitosan and hyaluronic acid)	922:981	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	0	45	theme	Immune	58:63	arg1	Response					65:72	the Immune Response	54:72	the Immune Response against an HIV Peptide Antigen	54:103	Polysaccharide Nanoparticles Can Efficiently Modulate the Immune Response against an HIV Peptide Antigen.
30964270	4	46	with	attachment	689:698	arg1	polyinosinic					795:806	polyinosinic	795:806	polyinosinic:polycytidylic acid (poly(I:C))	795:837	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	47	theme	I	833:833	arg1	C					835:835	I:C	833:835	I:C	833:835	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	4	47	theme	I	833:833	arg1	poly					828:831	poly	828:831	poly(I:C)	828:836	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	10	48	theme	nanoparticle	1570:1581	arg1	composition					1583:1593	the nanoparticle composition	1566:1593	the nanoparticle composition	1566:1593	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	6	49	used	used	1212:1215	arg2	Nanoparticles					1154:1166	Nanoparticles	1154:1166	Nanoparticles associating PCS5 by ionic interactions	1154:1205	Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation.
30964270	6	49	used	used	1212:1215	arg2	formulation					1246:1256	the control formulation	1234:1256	the control formulation	1234:1256	Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation.
30964270	8	50	theme	strongest	1396:1404	arg1	activation					1406:1415	the strongest activation	1392:1415	the strongest activation of antigen-presenting cells	1392:1443	Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells.
30964270	10	51	theme	cellular	1702:1709	arg1	responses					1711:1719	humoral and cellular responses	1690:1719	humoral and cellular responses	1690:1719	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	1	52	theme	effective	128:136	arg1	vaccine					142:148	an effective HIV vaccine	125:148	an effective HIV vaccine	125:148	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	4	53	with	coadministration	773:788	arg1	polyinosinic					795:806	polyinosinic	795:806	polyinosinic:polycytidylic acid (poly(I:C))	795:837	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	7	54	theme	high	1293:1296	arg1	antibodies					1308:1317	high anti-PCS5 antibodies	1293:1317	high anti-PCS5 antibodies	1293:1317	In vivo, all nanosystems elicited high anti-PCS5 antibodies.
30964270	1	55	theme	HIV	138:140	arg1	vaccine					142:148	an effective HIV vaccine	125:148	an effective HIV vaccine	125:148	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
30964270	5	56	theme	hyaluronic	966:975	arg1	acid					977:980	hyaluronic acid	966:980	hyaluronic acid	966:980	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	56	theme	hyaluronic	966:975	arg1	polysaccharides					936:950	two different polysaccharides	922:950	two different polysaccharides (chitosan and hyaluronic acid)	922:981	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	10	57	theme	humoral	1690:1696	arg1	responses					1711:1719	humoral and cellular responses	1690:1719	humoral and cellular responses	1690:1719	These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses.
30964270	2	58	theme	sites	341:345	arg1	strategy					381:388	a strategy	379:388	a strategy seeking to hamper the maturation and infectivity of HIV	379:444	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	58	theme	sites	341:345	arg1	targeting					286:294	the targeting	282:294	the targeting of the 12 highly conserved protease cleavage sites (PCS1-12)	282:355	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	5	59	dep	polysaccharides	936:950	arg1	chitosan					953:960	chitosan	953:960	chitosan	953:960	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	59	dep	polysaccharides	936:950	arg1	acid					977:980	hyaluronic acid	966:980	hyaluronic acid	966:980	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	59	dep	polysaccharides	936:950	arg1	polysaccharides					936:950	two different polysaccharides	922:950	two different polysaccharides (chitosan and hyaluronic acid)	922:981	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	5	60	theme	cleavable	1012:1020	arg1	bond					1022:1025	a cleavable bond	1010:1025	a cleavable bond	1010:1025	To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles.
30964270	4	61	theme	polysaccharide-based	727:746	arg1	nanoparticles					748:760	polysaccharide-based nanoparticles	727:760	polysaccharide-based nanoparticles	727:760	More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response.
30964270	2	62	theme	cleavage	332:339	arg1	PCS1-12					348:354	PCS1-12	348:354	PCS1-12	348:354	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	2	62	theme	cleavage	332:339	arg1	sites					341:345	the 12 highly conserved protease cleavage sites	299:345	the 12 highly conserved protease cleavage sites (PCS1-12)	299:355	Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1-12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV.
30964270	1	63	theme	RV144	215:219	arg1	trial					221:225	only the RV144 trial	206:225	only the RV144 trial	206:225	The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy.
31648129	5	0	from	gum	1033:1035	arg1	compositions					1003:1014	the distinct hydrolysis product compositions	971:1014	the distinct hydrolysis product compositions from locust bean gum and guar gum	971:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	5	1	theme	Docking	810:816	arg1	analysis					818:825	Docking analysis	810:825	Docking analysis	810:825	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	1	2	theme	thermophilic	181:192	arg1	ManAK					223:227	ManAK	223:227	ManAK	223:227	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	1	2	theme	thermophilic	181:192	arg1	β-mannanase					210:220	An engineered thermophilic and acidophilic β-mannanase	167:220	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308	167:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	4	3	theme	locust	598:603	arg1	gum					610:612	locust bean gum	598:612	locust bean gum	598:612	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	0	4	from	expression	11:20	arg1	kawachii					85:92	Aspergillus kawachii	73:92	Aspergillus kawachii	73:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	3	5	with	tolerant	531:538	arg1	optimum					550:556	a pH optimum	545:556	a pH optimum of 2.0	545:563	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	6	6	theme	ManAK	1080:1084	arg1	properties					1066:1075	These superior properties	1051:1075	These superior properties of ManAK	1051:1084	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	5	7	theme	distinct	975:982	arg1	compositions					1003:1014	the distinct hydrolysis product compositions	971:1014	the distinct hydrolysis product compositions from locust bean gum and guar gum	971:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	2	8	theme	cell	319:322	arg1	density					324:330	high cell density	314:330	high cell density fermentation	314:343	Through high cell density fermentation, the maximum yield reached 11,600 U/mL and 15.5 g/L, which is higher than most extreme β-mannanases.
31648129	4	9	theme	molecular	651:659	arg1	<2000 Da					683:690	<2000 Da	683:690	<2000 Da	683:690	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	9	theme	molecular	651:659	arg1	mannooligosaccharide					661:680	small molecular mannooligosaccharide	645:680	small molecular mannooligosaccharide (<2000 Da)	645:691	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	1	10	theme	Pichia	289:294	arg1	pastoris					296:303	Pichia pastoris	289:303	Pichia pastoris	289:303	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	3	11	theme	2.0	561:563	arg1	optimum					550:556	a pH optimum	545:556	a pH optimum of 2.0	545:563	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	2	12	theme	high	314:317	arg1	density					324:330	high cell density	314:330	high cell density fermentation	314:343	Through high cell density fermentation, the maximum yield reached 11,600 U/mL and 15.5 g/L, which is higher than most extreme β-mannanases.
31648129	3	13	theme	80 °C	515:519	arg1	optimum					504:510	a temperature optimum	490:510	a temperature optimum of 80 °C	490:519	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	3	14	theme	pH	547:548	arg1	optimum					550:556	a pH optimum	545:556	a pH optimum of 2.0	545:563	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	1	15	theme	acidophilic	198:208	arg1	ManAK					223:227	ManAK	223:227	ManAK	223:227	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	1	15	theme	acidophilic	198:208	arg1	β-mannanase					210:220	An engineered thermophilic and acidophilic β-mannanase	167:220	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308	167:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	0	16	theme	significant	108:118	arg1	potential					120:128	significant potential	108:128	significant potential in mannooligosaccharide preparation	108:164	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	5	17	from	residue	917:923	arg1	subsite					928:934	subsite -1	928:937	subsite -1	928:937	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	4	18	theme	small	645:649	arg1	<2000 Da					683:690	<2000 Da	683:690	<2000 Da	683:690	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	18	theme	small	645:649	arg1	mannooligosaccharide					661:680	small molecular mannooligosaccharide	645:680	small molecular mannooligosaccharide (<2000 Da)	645:691	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	3	19	theme	recombinant	450:460	arg1	thermostable					472:483	thermostable	472:483	thermostable	472:483	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	3	19	theme	recombinant	450:460	arg1	ManAK					462:466	The recombinant ManAK	446:466	The recombinant ManAK	446:466	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	0	20	theme	High-level	0:9	arg1	expression					11:20	High-level expression	0:20	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	0:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	4	21	theme	initial	707:713	arg1	concentration					725:737	high initial substrate concentration	702:737	high initial substrate concentration (10%)	702:743	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	21	theme	initial	707:713	arg1	%					742:742	10%	740:742	10%	740:742	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	6	22	theme	superior	1057:1064	arg1	properties					1066:1075	These superior properties	1051:1075	These superior properties of ManAK	1051:1084	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	0	23	theme	mannooligosaccharide	133:152	arg1	preparation					154:164	mannooligosaccharide preparation	133:164	mannooligosaccharide preparation	133:164	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	3	24	theme	temperature	492:502	arg1	optimum					504:510	a temperature optimum	490:510	a temperature optimum of 80 °C	490:519	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	4	25	from	distributions	773:785	arg1	products					800:807	their end products	790:807	their end products	790:807	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	2	26	theme	density	324:330	arg1	fermentation					332:343	high cell density fermentation	314:343	high cell density fermentation	314:343	Through high cell density fermentation, the maximum yield reached 11,600 U/mL and 15.5 g/L, which is higher than most extreme β-mannanases.
31648129	0	27	from	kawachii	85:92	arg1	expression					11:20	High-level expression	0:20	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	0:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	0	27	from	kawachii	85:92	arg1	β-mannanase					56:66	a thermophilic and acidophilic β-mannanase	25:66	a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	25:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	4	28	theme	end	796:798	arg1	products					800:807	their end products	790:807	their end products	790:807	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	1	29	theme	Aspergillus	235:245	arg1	IFO					256:258	Aspergillus kawachii IFO 4308	235:263	Aspergillus kawachii IFO 4308	235:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	0	30	theme	thermophilic	27:38	arg1	β-mannanase					56:66	a thermophilic and acidophilic β-mannanase	25:66	a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	25:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	6	31	from	preparation	1127:1137	arg1	feed					1167:1170	feed	1167:1170	feed	1167:1170	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	6	31	from	preparation	1127:1137	arg1	food					1158:1161	food	1158:1161	food	1158:1161	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	2	32	theme	extreme	424:430	arg1	β-mannanases					432:443	most extreme β-mannanases	419:443	most extreme β-mannanases	419:443	Through high cell density fermentation, the maximum yield reached 11,600 U/mL and 15.5 g/L, which is higher than most extreme β-mannanases.
31648129	0	33	theme	β-mannanase	56:66	arg1	expression					11:20	High-level expression	0:20	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	0:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	5	34	theme	locust	1021:1026	arg1	gum					1033:1035	locust bean gum	1021:1035	locust bean gum	1021:1035	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	4	35	theme	high	702:705	arg1	concentration					725:737	high initial substrate concentration	702:737	high initial substrate concentration (10%)	702:743	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	35	theme	high	702:705	arg1	%					742:742	10%	740:742	10%	740:742	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	0	36	theme	acidophilic	44:54	arg1	β-mannanase					56:66	a thermophilic and acidophilic β-mannanase	25:66	a thermophilic and acidophilic β-mannanase from Aspergillus kawachii	25:92	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	6	37	dep	food	1158:1161	arg1	area					1172:1175	area	1172:1175	area	1172:1175	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	0	38	from	potential	120:128	arg1	preparation					154:164	mannooligosaccharide preparation	133:164	mannooligosaccharide preparation	133:164	High-level expression of a thermophilic and acidophilic β-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation.
31648129	5	39	theme	bean	1028:1031	arg1	gum					1033:1035	locust bean gum	1021:1035	locust bean gum	1021:1035	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	6	40	from	application	1143:1153	arg1	feed					1167:1170	feed	1167:1170	feed	1167:1170	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	6	40	from	application	1143:1153	arg1	food					1158:1161	food	1158:1161	food	1158:1161	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	5	41	theme	galactopyranosyl	900:915	arg1	residue					917:923	a galactopyranosyl residue	898:923	a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum	898:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	1	42	from	IFO	256:258	arg1	ManAK					223:227	ManAK	223:227	ManAK	223:227	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	1	42	from	IFO	256:258	arg1	β-mannanase					210:220	An engineered thermophilic and acidophilic β-mannanase	167:220	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308	167:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	4	43	theme	Mw	770:771	arg1	distributions					773:785	different Mw distributions	760:785	different Mw distributions in their end products	760:807	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	44	theme	substrate	715:723	arg1	concentration					725:737	high initial substrate concentration	702:737	high initial substrate concentration (10%)	702:743	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	4	44	theme	substrate	715:723	arg1	%					742:742	10%	740:742	10%	740:742	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	6	45	theme	mannooligosaccharide	1106:1125	arg1	preparation					1127:1137	preparation	1127:1137	preparation	1127:1137	These superior properties of ManAK strongly facilitate mannooligosaccharide preparation and application in food and feed area.
31648129	4	46	theme	konjac	615:620	arg1	gum					622:624	konjac gum	615:624	konjac gum	615:624	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	3	47	with	thermostable	472:483	arg1	optimum					504:510	a temperature optimum	490:510	a temperature optimum of 80 °C	490:519	The recombinant ManAK was thermostable with a temperature optimum of 80 °C, and acid tolerant with a pH optimum of 2.0.
31648129	5	48	theme	guar	1041:1044	arg1	gum					1046:1048	guar gum	1041:1048	guar gum	1041:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	4	49	theme	different	760:768	arg1	distributions					773:785	different Mw distributions	760:785	different Mw distributions in their end products	760:807	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	2	50	theme	maximum	350:356	arg1	yield					358:362	the maximum yield	346:362	the maximum yield	346:362	Through high cell density fermentation, the maximum yield reached 11,600 U/mL and 15.5 g/L, which is higher than most extreme β-mannanases.
31648129	1	51	theme	kawachii	247:254	arg1	IFO					256:258	Aspergillus kawachii IFO 4308	235:263	Aspergillus kawachii IFO 4308	235:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	5	52	theme	hydrolysis	984:993	arg1	compositions					1003:1014	the distinct hydrolysis product compositions	971:1014	the distinct hydrolysis product compositions from locust bean gum and guar gum	971:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	4	53	theme	guar	631:634	arg1	gum					636:638	guar gum	631:638	guar gum	631:638	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	5	54	theme	ManAK	869:873	arg1	pocket					859:864	the catalytic pocket	845:864	the catalytic pocket of ManAK	845:873	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	5	55	theme	catalytic	849:857	arg1	pocket					859:864	the catalytic pocket	845:864	the catalytic pocket of ManAK	845:873	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	4	56	theme	bean	605:608	arg1	gum					610:612	locust bean gum	598:612	locust bean gum	598:612	ManAK could efficiently degrade locust bean gum, konjac gum, and guar gum into small molecular mannooligosaccharide (<2000 Da), even at high initial substrate concentration (10%), and displayed different Mw distributions in their end products.
31648129	5	57	from	gum	1046:1048	arg1	compositions					1003:1014	the distinct hydrolysis product compositions	971:1014	the distinct hydrolysis product compositions from locust bean gum and guar gum	971:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	5	58	theme	product	995:1001	arg1	compositions					1003:1014	the distinct hydrolysis product compositions	971:1014	the distinct hydrolysis product compositions from locust bean gum and guar gum	971:1048	Docking analysis demonstrated that the catalytic pocket of ManAK could only accommodate a galactopyranosyl residue in subsite -1, which might be responsible for the distinct hydrolysis product compositions from locust bean gum and guar gum.
31648129	1	59	theme	engineered	170:179	arg1	ManAK					223:227	ManAK	223:227	ManAK	223:227	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
31648129	1	59	theme	engineered	170:179	arg1	β-mannanase					210:220	An engineered thermophilic and acidophilic β-mannanase	167:220	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308	167:263	An engineered thermophilic and acidophilic β-mannanase (ManAK) from Aspergillus kawachii IFO 4308 was highly expressed in Pichia pastoris.
30889794	7	0	theme	gut	1210:1212	arg1	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	0	theme	gut	1210:1212	arg1	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	0	theme	gut	1210:1212	arg1	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	0	theme	gut	1210:1212	arg1	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	0	theme	gut	1210:1212	arg1	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	4	1	theme	Linear	681:686	arg1	analysis					701:708	Linear discriminant analysis	681:708	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	1	2	theme	hydrolysis	242:251	arg1	method					253:258	specific enzymatic hydrolysis method	223:258	specific enzymatic hydrolysis method	223:258	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	0	3	theme	Enzymatic	78:86	arg1	Hydrolysis					88:97	Enzymatic Hydrolysis	78:97	Enzymatic Hydrolysis	78:97	Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis.
30889794	3	4	theme	microbiota	657:666	arg1	composition					668:678	the microbiota composition	653:678	the microbiota composition	653:678	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	6	5	theme	microbial	1088:1096	arg1	producers					1098:1106	SCFA microbial producers	1083:1106	SCFA microbial producers	1083:1106	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	4	6	theme	supplemented	876:887	arg1	medium					889:894	AlgO supplemented medium	871:894	AlgO supplemented medium	871:894	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	7	7	theme	gastrointestinal	1276:1291	arg1	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	7	theme	gastrointestinal	1276:1291	arg1	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	7	theme	gastrointestinal	1276:1291	arg1	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	7	theme	gastrointestinal	1276:1291	arg1	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	7	theme	gastrointestinal	1276:1291	arg1	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	4	8	theme	AlgO	871:874	arg1	medium					889:894	AlgO supplemented medium	871:894	AlgO supplemented medium	871:894	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	6	9	theme	producers	1098:1106	arg1	abundance					1070:1078	the abundance	1066:1078	the abundance of SCFA microbial producers and opportunistic pathogenic flora	1066:1141	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	3	10	theme	fecal	477:481	arg1	microbiota					483:492	pig fecal microbiota	473:492	pig fecal microbiota	473:492	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	4	11	theme	analysis	701:708	arg1	LEfSe					723:727	LEfSe	723:727	LEfSe	723:727	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	11	theme	analysis	701:708	arg1	size					717:720	Linear discriminant analysis effect size	681:720	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	2	12	theme	weight	275:280	arg1	distributions					282:294	The molecular weight distributions	261:294	The molecular weight distributions of the three oligosaccharides	261:324	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	2	12	theme	weight	275:280	arg1	kDa					339:341	1.0⁻5.0 kDa	331:341	1.0⁻5.0 kDa	331:341	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	5	13	theme	gut	918:920	arg1	composition					933:943	the gut microbiota composition	914:943	the gut microbiota composition	914:943	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	6	14	theme	SCFA	1083:1086	arg1	producers					1098:1106	SCFA microbial producers	1083:1106	SCFA microbial producers	1083:1106	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	0	15	theme	Marine	43:48	arg1	Algae					50:54	Three Marine Algae	37:54	Three Marine Algae	37:54	Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis.
30889794	6	16	theme	flora	1137:1141	arg1	abundance					1070:1078	the abundance	1066:1078	the abundance of SCFA microbial producers and opportunistic pathogenic flora	1066:1141	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	2	17	theme	molecular	265:273	arg1	distributions					282:294	The molecular weight distributions	261:294	The molecular weight distributions of the three oligosaccharides	261:324	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	2	17	theme	molecular	265:273	arg1	kDa					339:341	1.0⁻5.0 kDa	331:341	1.0⁻5.0 kDa	331:341	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	7	18	theme	microbial	1214:1222	arg1	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	18	theme	microbial	1214:1222	arg1	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	18	theme	microbial	1214:1222	arg1	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	18	theme	microbial	1214:1222	arg1	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	18	theme	microbial	1214:1222	arg1	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	1	19	theme	κ-carrageenan	169:181	arg1	KCO					201:203	KCO	201:203	KCO	201:203	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	1	19	theme	κ-carrageenan	169:181	arg1	oligosaccharides					183:198	κ-carrageenan oligosaccharides	169:198	κ-carrageenan oligosaccharides (KCO)	169:204	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	4	20	theme	effect	710:715	arg1	LEfSe					723:727	LEfSe	723:727	LEfSe	723:727	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	20	theme	effect	710:715	arg1	size					717:720	Linear discriminant analysis effect size	681:720	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	2	21	theme	oligosaccharides	309:324	arg1	distributions					282:294	The molecular weight distributions	261:294	The molecular weight distributions of the three oligosaccharides	261:324	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	2	21	theme	oligosaccharides	309:324	arg1	kDa					339:341	1.0⁻5.0 kDa	331:341	1.0⁻5.0 kDa	331:341	The molecular weight distributions of the three oligosaccharides were 1.0⁻5.0 kDa, 0.4⁻1.4 kDa, and 1.0⁻7.0 kDa, respectively.
30889794	7	22	theme	physiological	1337:1349	arg1	function					1351:1358	the physiological function	1333:1358	the physiological function of KCO	1333:1365	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	0	23	theme	Potential	24:32	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Prebiotic Potential of Three Marine Algae	0:54	Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis.
30889794	5	24	theme	Roseburia	1005:1013	arg1	abundance					962:970	the abundance	958:970	the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium	958:1035	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	5	25	theme	microbiota	922:931	arg1	composition					933:943	the gut microbiota composition	914:943	the gut microbiota composition	914:943	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	4	26	theme	analysis	730:737	arg1	results					739:745	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	1	27	theme	Alginate	100:107	arg1	AlgO					127:130	AlgO	127:130	AlgO	127:130	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	1	27	theme	Alginate	100:107	arg1	oligosaccharides					109:124	Alginate oligosaccharides	100:124	Alginate oligosaccharides (AlgO)	100:131	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	5	28	theme	Ruminococcaceae	975:989	arg1	abundance					962:970	the abundance	958:970	the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium	958:1035	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	3	29	theme	butyric	626:632	arg1	acid					634:637	butyric acid	626:637	especially butyric acid	615:637	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	29	theme	butyric	626:632	arg1	acids					600:604	short-chain fatty acids	582:604	short-chain fatty acids (SCFAs)	582:612	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	30	theme	short-chain	582:592	arg1	acid					634:637	butyric acid	626:637	especially butyric acid	615:637	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	30	theme	short-chain	582:592	arg1	SCFAs					607:611	SCFAs	607:611	SCFAs	607:611	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	30	theme	short-chain	582:592	arg1	acids					600:604	short-chain fatty acids	582:604	short-chain fatty acids (SCFAs)	582:612	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	7	31	theme	gut	1312:1314	arg1	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	31	theme	gut	1312:1314	arg1	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	31	theme	gut	1312:1314	arg1	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	31	theme	gut	1312:1314	arg1	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	31	theme	gut	1312:1314	arg1	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	3	32	theme	fatty	594:598	arg1	acid					634:637	butyric acid	626:637	especially butyric acid	615:637	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	32	theme	fatty	594:598	arg1	SCFAs					607:611	SCFAs	607:611	SCFAs	607:611	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	32	theme	fatty	594:598	arg1	acids					600:604	short-chain fatty acids	582:604	short-chain fatty acids (SCFAs)	582:612	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	0	33	theme	Algae	50:54	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Prebiotic Potential of Three Marine Algae	0:54	Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis.
30889794	7	34	used	used	1202:1205	arg2	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	34	used	used	1202:1205	arg2	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	34	used	used	1202:1205	arg2	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	34	used	used	1202:1205	arg2	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	34	used	used	1202:1205	arg2	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	35	theme	KCO	1363:1365	arg1	function					1351:1358	the physiological function	1333:1358	the physiological function of KCO	1333:1365	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	3	36	dep	h.	511:512	arg1	capable					539:545	capable	539:545	capable	539:545	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	3	37	theme	culture	392:398	arg1	medium					400:405	The culture medium	388:405	The culture medium	388:405	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	4	38	theme	discriminant	688:699	arg1	analysis					701:708	Linear discriminant analysis	681:708	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	7	39	dep	used	1202:1205	arg1	whereas					1325:1331	whereas	1325:1331	whereas	1325:1331	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	5	40	theme	Coprococcus	992:1002	arg1	abundance					962:970	the abundance	958:970	the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium	958:1035	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	4	41	theme	pathogenic	777:786	arg1	bacteria					788:795	the opportunistic pathogenic bacteria	759:795	the opportunistic pathogenic bacteria Escherichia	759:807	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	41	theme	pathogenic	777:786	arg1	Shigella					810:817	Shigella	810:817	Shigella	810:817	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	41	theme	pathogenic	777:786	arg1	Peptoniphilus					824:836	Peptoniphilus	824:836	Peptoniphilus	824:836	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	5	42	theme	Faecalibacterium	1020:1035	arg1	abundance					962:970	the abundance	958:970	the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium	958:1035	AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium.
30889794	3	43	theme	pig	473:475	arg1	microbiota					483:492	pig fecal microbiota	473:492	pig fecal microbiota	473:492	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	6	44	theme	pathogenic	1126:1135	arg1	flora					1137:1141	opportunistic pathogenic flora	1112:1141	opportunistic pathogenic flora	1112:1141	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	4	45	theme	opportunistic	763:775	arg1	bacteria					788:795	the opportunistic pathogenic bacteria	759:795	the opportunistic pathogenic bacteria Escherichia	759:807	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	45	theme	opportunistic	763:775	arg1	Shigella					810:817	Shigella	810:817	Shigella	810:817	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	4	45	theme	opportunistic	763:775	arg1	Peptoniphilus					824:836	Peptoniphilus	824:836	Peptoniphilus	824:836	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	1	46	theme	agarose	134:140	arg1	AO					160:161	AO	160:161	AO	160:161	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	1	46	theme	agarose	134:140	arg1	oligosaccharides					142:157	agarose oligosaccharides	134:157	agarose oligosaccharides (AO)	134:162	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	4	47	theme	size	717:720	arg1	results					739:745	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results	681:745	Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium.
30889794	3	48	theme	acids	600:604	arg1	concentration					565:577	the concentration	561:577	the concentration of short-chain fatty acids (SCFAs), especially butyric acid,	561:638	The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition.
30889794	6	49	theme	opportunistic	1112:1124	arg1	flora					1137:1141	opportunistic pathogenic flora	1112:1141	opportunistic pathogenic flora	1112:1141	Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora.
30889794	1	50	theme	specific	223:230	arg1	method					253:258	specific enzymatic hydrolysis method	223:258	specific enzymatic hydrolysis method	223:258	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
30889794	7	51	theme	further	1373:1379	arg1	evaluation					1381:1390	further evaluation	1373:1390	further evaluation	1373:1390	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	0	52	theme	Prebiotic	14:22	arg1	Potential					24:32	Prebiotic Potential	14:32	Prebiotic Potential	14:32	Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis.
30889794	7	53	theme	animal/human	1263:1274	arg1	health					1293:1298	animal/human gastrointestinal health	1263:1298	animal/human gastrointestinal health	1263:1298	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	53	theme	animal/human	1263:1274	arg1	AO					1192:1193	AO	1192:1193	AO	1192:1193	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	53	theme	animal/human	1263:1274	arg1	regulators					1224:1233	gut microbial regulators	1210:1233	gut microbial regulators	1210:1233	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	53	theme	animal/human	1263:1274	arg1	disease					1316:1322	gut disease	1312:1322	gut disease	1312:1322	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	7	53	theme	animal/human	1263:1274	arg1	AlgO					1183:1186	AlgO	1183:1186	AlgO	1183:1186	Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
30889794	1	54	theme	enzymatic	232:240	arg1	method					253:258	specific enzymatic hydrolysis method	223:258	specific enzymatic hydrolysis method	223:258	Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method.
31839353	3	0	theme	general	1013:1019	arg1	increase					1077:1084	the increase	1073:1084	the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1073:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	0	theme	general	1013:1019	arg1	tendency					1021:1028	a general tendency	1011:1028	a general tendency	1011:1028	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	5	1	theme	acetonitrile	1605:1616	arg1	content					1618:1624	acetonitrile content	1605:1624	acetonitrile content	1605:1624	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	2	theme	eluent	1417:1422	arg1	systems					1424:1430	eluent systems	1417:1430	eluent systems composed of methanol and acetonitrile with organic acid and base additives	1417:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	3	3	theme	polysaccharide	1045:1058	arg1	SOs					1060:1062	all polysaccharide SOs	1041:1062	all polysaccharide SOs studied	1041:1070	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	2	4	theme	bulk	887:890	arg1	solvent					892:898	the bulk solvent	883:898	the bulk solvent	883:898	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	7	5	theme	Thermodynamic	1795:1807	arg1	parameters					1809:1818	Thermodynamic parameters	1795:1818	Thermodynamic parameters derived from temperature dependence studies	1795:1862	Thermodynamic parameters derived from temperature dependence studies revealed that separations are controlled by enthalpy.
31839353	1	6	theme	stationary	562:571	arg1	phases					573:578	chiral stationary phases	555:578	chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	555:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	6	theme	stationary	562:571	arg1	CSPs					581:584	CSPs	581:584	CSPs	581:584	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	2	7	from	retention	975:983	arg1	investigation					832:844	the investigation	828:844	the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	828:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	8	theme	CSP	816:818	arg1	types					807:811	both types	802:811	both types of CSP	802:818	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	9	from	enantiorecognition	780:797	arg1	types					807:811	both types	802:811	both types of CSP	802:818	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	0	10	theme	fluid	266:270	arg1	chromatography					272:285	high-performance liquid and subcritical fluid chromatography	226:285	chromatography	272:285	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	2	11	theme	effects	853:859	arg1	investigation					832:844	the investigation	828:844	the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	828:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	0	12	theme	phases	146:151	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance	0:110	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	12	theme	phases	146:151	arg1	acid					191:194	sulfonic acid	182:194	sulfonic acid	182:194	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	12	theme	phases	146:151	arg1	Utilization					113:123	Utilization	113:123	Utilization of chiral stationary phases based on polysaccharides	113:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	7	13	theme	temperature	1833:1843	arg1	studies					1856:1862	temperature dependence studies	1833:1862	temperature dependence studies	1833:1862	Thermodynamic parameters derived from temperature dependence studies revealed that separations are controlled by enthalpy.
31839353	1	14	theme	analogues	493:501	arg1	enantiomers					398:408	the enantiomers	394:408	the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest	394:529	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	3	15	from	concentration	1093:1105	arg1	phase					1145:1149	the mobile phase	1134:1149	the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1134:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	6	16	theme	high	1671:1674	arg1	increase					1676:1683	an extremely high increase	1658:1683	an extremely high increase in retention	1658:1696	However, under SFC conditions, an extremely high increase in retention was observed with increased CO2 content, while selectivity and resolution changed only slightly.
31839353	2	17	theme	peculiarities	759:771	arg1	influence					735:743	the influence	731:743	the influence of structural peculiarities on the enantiorecognition on both types of CSP	731:818	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	18	theme	Cinchona	676:683	arg1	alkaloids					685:693	Cinchona alkaloids	676:693	Cinchona alkaloids	676:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	19	theme	pharmacological	506:520	arg1	interest					522:529	pharmacological interest	506:529	pharmacological interest	506:529	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	5	20	from	increases	1517:1525	arg1	resolution					1564:1573	resolution	1564:1573	resolution	1564:1573	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	20	from	increases	1517:1525	arg1	selectivity					1548:1558	selectivity	1548:1558	selectivity	1548:1558	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	20	from	increases	1517:1525	arg1	factor					1540:1545	retention factor	1530:1545	retention factor	1530:1545	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	2	21	from	influence	735:743	arg1	enantiorecognition					780:797	the enantiorecognition	776:797	the enantiorecognition on both types of CSP	776:818	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	5	22	theme	organic	1475:1481	arg1	acid					1483:1486	organic acid	1475:1486	organic acid	1475:1486	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	0	23	theme	importance	101:110	arg1	ß-carboline					21:31	ß-carboline	21:31	ß-carboline	21:31	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	23	theme	importance	101:110	arg1	tetrahydroisoquinoline					34:55	tetrahydroisoquinoline	34:55	tetrahydroisoquinoline	34:55	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	23	theme	importance	101:110	arg1	analogues					73:81	benzazepine analogues	61:81	benzazepine analogues	61:81	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	3	24	theme	concentration	1093:1105	arg1	increase					1077:1084	the increase	1073:1084	the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1073:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	24	theme	concentration	1093:1105	arg1	tendency					1021:1028	a general tendency	1011:1028	a general tendency	1011:1028	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	1	25	theme	diverse	426:432	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	6	26	theme	SFC	1642:1644	arg1	conditions					1646:1655	SFC conditions	1642:1655	SFC conditions	1642:1655	However, under SFC conditions, an extremely high increase in retention was observed with increased CO2 content, while selectivity and resolution changed only slightly.
31839353	3	27	from	phase	1145:1149	arg1	increase					1077:1084	the increase	1073:1084	the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1073:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	27	from	phase	1145:1149	arg1	tendency					1021:1028	a general tendency	1011:1028	a general tendency	1011:1028	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	6	28	theme	CO2	1726:1728	arg1	content					1730:1736	increased CO2 content	1716:1736	increased CO2 content	1716:1736	However, under SFC conditions, an extremely high increase in retention was observed with increased CO2 content, while selectivity and resolution changed only slightly.
31839353	2	29	theme	work	712:715	arg1	aim					700:702	The aim	696:702	The aim of this work	696:715	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	0	30	theme	chiral	128:133	arg1	phases					146:151	chiral stationary phases	128:151	chiral stationary phases based on polysaccharides	128:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	31	theme	basic	435:439	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	3	32	theme	component	1121:1129	arg1	concentration					1093:1105	the concentration	1089:1105	the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1089:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	2	33	from	stereoselectivity	989:1005	arg1	investigation					832:844	the investigation	828:844	the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	828:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	34	theme	subcritical	339:349	arg1	separations					379:389	subcritical fluid chromatographic (SFC) separations	339:389	subcritical fluid chromatographic (SFC) separations	339:389	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	2	35	theme	selectors	956:964	arg1	effects					853:859	the effects	849:859	the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	849:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	36	theme	sulfonic	647:654	arg1	derivatives					661:671	(ii) zwitterionic sulfonic acid derivatives	629:671	(ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	629:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	0	37	theme	ß-carboline	21:31	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance	0:110	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	37	theme	ß-carboline	21:31	arg1	acid					191:194	sulfonic acid	182:194	sulfonic acid	182:194	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	37	theme	ß-carboline	21:31	arg1	Utilization					113:123	Utilization	113:123	Utilization of chiral stationary phases based on polysaccharides	113:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	38	theme	chromatographic	357:371	arg1	separations					379:389	subcritical fluid chromatographic (SFC) separations	339:389	subcritical fluid chromatographic (SFC) separations	339:389	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	0	39	theme	tetrahydroisoquinoline	34:55	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance	0:110	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	39	theme	tetrahydroisoquinoline	34:55	arg1	acid					191:194	sulfonic acid	182:194	sulfonic acid	182:194	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	39	theme	tetrahydroisoquinoline	34:55	arg1	Utilization					113:123	Utilization	113:123	Utilization of chiral stationary phases based on polysaccharides	113:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	40	theme	SFC	374:376	arg1	separations					379:389	subcritical fluid chromatographic (SFC) separations	339:389	subcritical fluid chromatographic (SFC) separations	339:389	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	6	41	from	increase	1676:1683	arg1	retention					1688:1696	retention	1688:1696	retention	1688:1696	However, under SFC conditions, an extremely high increase in retention was observed with increased CO2 content, while selectivity and resolution changed only slightly.
31839353	2	42	from	investigation	832:844	arg1	stereoselectivity					989:1005	stereoselectivity	989:1005	stereoselectivity	989:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	42	from	investigation	832:844	arg1	retention					975:983	retention	975:983	retention	975:983	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	5	43	located	observed	1580:1587	arg1	mode					1402:1405	polar ionic mode	1390:1405	polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives	1390:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	43	located	observed	1580:1587	arg2	increases					1517:1525	moderate increases	1508:1525	moderate increases in retention factor, selectivity and resolution	1508:1573	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	0	44	theme	benzazepine	61:71	arg1	analogues					73:81	benzazepine analogues	61:81	benzazepine analogues	61:81	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	5	45	theme	moderate	1508:1515	arg1	increases					1517:1525	moderate increases	1508:1525	moderate increases in retention factor, selectivity and resolution	1508:1573	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	46	theme	polar	1390:1394	arg1	mode					1402:1405	polar ionic mode	1390:1405	polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives	1390:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	7	47	theme	dependence	1845:1854	arg1	studies					1856:1862	temperature dependence studies	1833:1862	temperature dependence studies	1833:1862	Thermodynamic parameters derived from temperature dependence studies revealed that separations are controlled by enthalpy.
31839353	2	48	theme	composition	868:878	arg1	effects					853:859	the effects	849:859	the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	849:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	49	theme	structures	905:914	arg1	effects					853:859	the effects	849:859	the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity	849:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	3	50	theme	liquid	1171:1176	arg1	CO2					1178:1180	liquid CO2	1171:1180	liquid CO2 for SFC	1171:1188	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	2	51	theme	solvent	892:898	arg1	selectors					956:964	chiral selectors	949:964	chiral selectors (SOs)	949:970	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	51	theme	solvent	892:898	arg1	structures					905:914	the structures	901:914	the structures of the chiral analytes (SAs)	901:943	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	51	theme	solvent	892:898	arg1	composition					868:878	the composition	864:878	the composition of the bulk solvent	864:898	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	51	theme	solvent	892:898	arg1	SOs					967:969	SOs	967:969	SOs	967:969	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	4	52	theme	similar	1328:1334	arg1	behaviour					1336:1344	similar behaviour	1328:1344	similar behaviour	1328:1344	In a way, similar behaviour was registered for the zwitterionic SOs.
31839353	3	53	theme	valid	1031:1035	arg1	increase					1077:1084	the increase	1073:1084	the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1073:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	53	theme	valid	1031:1035	arg1	tendency					1021:1028	a general tendency	1011:1028	a general tendency	1011:1028	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	2	54	theme	analytes	930:937	arg1	selectors					956:964	chiral selectors	949:964	chiral selectors (SOs)	949:970	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	54	theme	analytes	930:937	arg1	structures					905:914	the structures	901:914	the structures of the chiral analytes (SAs)	901:943	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	54	theme	analytes	930:937	arg1	composition					868:878	the composition	864:878	the composition of the bulk solvent	864:898	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	54	theme	analytes	930:937	arg1	SOs					967:969	SOs	967:969	SOs	967:969	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	0	55	theme	subcritical	254:264	arg1	chromatography					272:285	high-performance liquid and subcritical fluid chromatography	226:285	chromatography	272:285	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	56	theme	chiral	555:560	arg1	phases					573:578	chiral stationary phases	555:578	chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	555:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	56	theme	chiral	555:560	arg1	CSPs					581:584	CSPs	581:584	CSPs	581:584	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	3	57	dep	phase	1145:1149	arg1	n-hexane					1152:1159	n-hexane	1152:1159	n-hexane for LC or liquid CO2 for SFC	1152:1188	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	5	58	theme	ionic	1396:1400	arg1	mode					1402:1405	polar ionic mode	1390:1405	polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives	1390:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	4	59	theme	zwitterionic	1369:1380	arg1	SOs					1382:1384	the zwitterionic SOs	1365:1384	the zwitterionic SOs	1365:1384	In a way, similar behaviour was registered for the zwitterionic SOs.
31839353	7	60	attach	derived	1820:1826	arg2	parameters					1809:1818	Thermodynamic parameters	1795:1818	Thermodynamic parameters derived from temperature dependence studies	1795:1862	Thermodynamic parameters derived from temperature dependence studies revealed that separations are controlled by enthalpy.
31839353	7	60	attach	derived	1820:1826	arg1	studies					1856:1862	temperature dependence studies	1833:1862	temperature dependence studies	1833:1862	Thermodynamic parameters derived from temperature dependence studies revealed that separations are controlled by enthalpy.
31839353	1	61	theme	tetrahydroisoquinoline	454:475	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	3	62	theme	most	1247:1250	arg1	cases					1252:1256	most cases	1247:1256	most cases	1247:1256	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	63	from	increase	1077:1084	arg1	phase					1145:1149	the mobile phase	1134:1149	the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1134:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	3	64	theme	increased	1280:1288	arg1	selectivity					1290:1300	increased selectivity	1280:1300	increased selectivity	1280:1300	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	0	65	theme	sulfonic	182:189	arg1	acid					191:194	sulfonic acid	182:194	sulfonic acid	182:194	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	66	theme	benzazepine	481:491	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	5	67	with	acetonitrile	1457:1468	arg1	acid					1483:1486	organic acid	1475:1486	organic acid	1475:1486	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	67	with	acetonitrile	1457:1468	arg1	additives					1497:1505	base additives	1492:1505	base additives	1492:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	68	with	methanol	1444:1451	arg1	acid					1483:1486	organic acid	1475:1486	organic acid	1475:1486	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	5	68	with	methanol	1444:1451	arg1	additives					1497:1505	base additives	1492:1505	base additives	1492:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	1	69	theme	alkaloids	685:693	arg1	derivatives					661:671	(ii) zwitterionic sulfonic acid derivatives	629:671	(ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	629:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	69	theme	alkaloids	685:693	arg1	polysaccharides-					608:623	(i) neutral polysaccharides-	596:623	(i) neutral polysaccharides-	596:623	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	0	70	theme	pharmaceutical	86:99	arg1	importance					101:110	pharmaceutical importance	86:110	pharmaceutical importance	86:110	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	1	71	theme	interest	522:529	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	72	theme	enantiomers	398:408	arg1	HPLC					329:332	HPLC	329:332	HPLC	329:332	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	72	theme	enantiomers	398:408	arg1	chromatographic					312:326	chromatographic	312:326	chromatographic	312:326	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	5	73	theme	base	1492:1495	arg1	additives					1497:1505	base additives	1492:1505	base additives	1492:1505	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	0	74	theme	stationary	135:144	arg1	phases					146:151	chiral stationary phases	128:151	chiral stationary phases based on polysaccharides	128:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	3	75	theme	apolar	1114:1119	arg1	component					1121:1129	the apolar component	1110:1129	the apolar component	1110:1129	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	6	76	theme	increased	1716:1724	arg1	content					1730:1736	increased CO2 content	1716:1736	increased CO2 content	1716:1736	However, under SFC conditions, an extremely high increase in retention was observed with increased CO2 content, while selectivity and resolution changed only slightly.
31839353	2	77	theme	structural	748:757	arg1	peculiarities					759:771	structural peculiarities	748:771	structural peculiarities	748:771	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	78	theme	ß-carboline	441:451	arg1	analogues					493:501	structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues	413:501	analogues	493:501	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	79	theme	zwitterionic	634:645	arg1	derivatives					661:671	(ii) zwitterionic sulfonic acid derivatives	629:671	(ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	629:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	1	80	theme	fluid	351:355	arg1	separations					379:389	subcritical fluid chromatographic (SFC) separations	339:389	subcritical fluid chromatographic (SFC) separations	339:389	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	3	81	theme	mobile	1138:1143	arg1	phase					1145:1149	the mobile phase	1134:1149	the mobile phase (n-hexane for LC or liquid CO2 for SFC)	1134:1189	As a general tendency, valid for all polysaccharide SOs studied, the increase of the concentration of the apolar component in the mobile phase (n-hexane for LC or liquid CO2 for SFC) was found to significantly increase retention, which in most cases, was accompanied with increased selectivity and resolution.
31839353	1	82	dep	polysaccharides-	608:623	arg1	i					597:597	i	597:597	i	597:597	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	2	83	theme	chiral	949:954	arg1	selectors					956:964	chiral selectors	949:964	chiral selectors (SOs)	949:970	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	83	theme	chiral	949:954	arg1	SOs					967:969	SOs	967:969	SOs	967:969	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	5	84	theme	retention	1530:1538	arg1	factor					1540:1545	retention factor	1530:1545	retention factor	1530:1545	In polar ionic mode employing eluent systems composed of methanol and acetonitrile with organic acid and base additives, moderate increases in retention factor, selectivity and resolution were observed with increasing acetonitrile content.
31839353	1	85	theme	acid	656:659	arg1	derivatives					661:671	(ii) zwitterionic sulfonic acid derivatives	629:671	(ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids	629:693	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	0	86	theme	analogues	73:81	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance	0:110	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	86	theme	analogues	73:81	arg1	acid					191:194	sulfonic acid	182:194	sulfonic acid	182:194	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	0	86	theme	analogues	73:81	arg1	Utilization					113:123	Utilization	113:123	Utilization of chiral stationary phases based on polysaccharides	113:176	Enantioseparation of ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmaceutical importance: Utilization of chiral stationary phases based on polysaccharides and sulfonic acid modified Cinchonaalkaloids in high-performance liquid and subcritical fluid chromatography.
31839353	2	87	theme	chiral	923:928	arg1	SAs					940:942	SAs	940:942	SAs	940:942	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	87	theme	chiral	923:928	arg1	analytes					930:937	the chiral analytes	919:937	the chiral analytes (SAs)	919:943	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	88	dep	derivatives	661:671	arg1	ii					630:631	ii	630:631	ii	630:631	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31839353	2	89	from	effects	853:859	arg1	stereoselectivity					989:1005	stereoselectivity	989:1005	stereoselectivity	989:1005	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	2	89	from	effects	853:859	arg1	retention					975:983	retention	975:983	retention	975:983	The aim of this work was to reveal the influence of structural peculiarities on the enantiorecognition on both types of CSP through the investigation of the effects of the composition of the bulk solvent, the structures of the chiral analytes (SAs) and chiral selectors (SOs) on retention and stereoselectivity.
31839353	1	90	theme	neutral	600:606	arg1	polysaccharides-					608:623	(i) neutral polysaccharides-	596:623	(i) neutral polysaccharides-	596:623	High-performance liquid chromatographic (HPLC) and subcritical fluid chromatographic (SFC) separations of the enantiomers of structurally diverse, basic ß-carboline, tetrahydroisoquinoline and benzazepine analogues of pharmacological interest were performed applying chiral stationary phases (CSPs) based on (i) neutral polysaccharides- and (ii) zwitterionic sulfonic acid derivatives of Cinchona alkaloids.
31604078	7	0	theme	hydrogen	878:885	arg1	bond					887:890	The hydrogen bond	874:890	The hydrogen bond	874:890	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	2	1	theme	%	302:302	arg1	acid					319:322	2 wt% glacial acetic acid	298:322	2 wt% glacial acetic acid	298:322	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	0	2	from	bonding	9:15	arg1	system					63:68	chitosan/Antarctic krill protein composite system	20:68	chitosan/Antarctic krill protein composite system	20:68	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	7	3	theme	water	1007:1011	arg1	molecules					1013:1021	the water molecules	1003:1021	the water molecules in blended fibers	1003:1039	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	6	4	dep	Fourier	832:838	arg1	transform					840:848	transform	840:848	transform infrared curve fitting	840:871	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	2	5	theme	2 wt	298:301	arg1	%					302:302	%	302:302	%	302:302	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	4	6	theme	tensile	503:509	arg1	2.53 cN/dtex					541:552	2.53 cN/dtex	541:552	2.53 cN/dtex	541:552	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	4	6	theme	tensile	503:509	arg1	strength					511:518	The tensile strength	499:518	The tensile strength of blended fibers	499:536	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	6	7	theme	hydrogen	797:804	arg1	bonds					806:810	the restructured hydrogen bonds	780:810	the restructured hydrogen bonds in CS/AKP system	780:827	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	5	8	theme	fibers	688:693	arg1	bond					723:726	the hydrogen bond	710:726	the hydrogen bond formed between CS and AKP	710:752	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	5	8	theme	fibers	688:693	arg1	strength					664:671	the increased tensile strength	642:671	the increased tensile strength of the blended fibers	642:693	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	1	9	theme	Antarctic	237:245	arg1	AKP					262:264	AKP	262:264	AKP	262:264	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	1	9	theme	Antarctic	237:245	arg1	protein					253:259	Antarctic krill protein	237:259	Antarctic krill protein (AKP)	237:265	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	6	10	theme	restructured	784:795	arg1	bonds					806:810	the restructured hydrogen bonds	780:810	the restructured hydrogen bonds in CS/AKP system	780:827	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	7	11	theme	blended	1026:1032	arg1	fibers					1034:1039	blended fibers	1026:1039	blended fibers	1026:1039	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	3	12	theme	lab-scale	467:475	arg1	machine					490:496	a lab-scale wet-spinning machine	465:496	a lab-scale wet-spinning machine	465:496	Then we obtained CS/AKP fiber by wet-spinning on a lab-scale wet-spinning machine.
31604078	1	13	theme	krill	247:251	arg1	AKP					262:264	AKP	262:264	AKP	262:264	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	1	13	theme	krill	247:251	arg1	protein					253:259	Antarctic krill protein	237:259	Antarctic krill protein (AKP)	237:265	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	7	14	theme	drivable	975:982	arg1	factor					984:989	the main drivable factor	966:989	the main drivable factor	966:989	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	2	15	theme	2 wt	329:332	arg1	glycol					344:349	2 wt% ethylene glycol	329:349	2 wt% ethylene glycol	329:349	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	2	16	theme	composite	396:404	arg1	solution					406:413	a stable CS/AKP composite solution	380:413	a stable CS/AKP composite solution	380:413	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	7	17	from	benefits	901:908	arg1	growth					925:930	crystalline growth	913:930	crystalline growth of CS along the fiber axis	913:957	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	6	18	from	bonds	806:810	arg1	system					822:827	CS/AKP system	815:827	CS/AKP system	815:827	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	0	19	dep	construction	80:91	arg1	mechanism					109:117	mechanism	109:117	mechanism	109:117	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	0	20	theme	Hydrogen	0:7	arg1	bonding					9:15	Hydrogen bonding	0:15	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.	0:118	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	7	21	theme	main	970:973	arg1	factor					984:989	the main drivable factor	966:989	the main drivable factor	966:989	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	0	22	theme	chitosan/Antarctic	20:37	arg1	system					63:68	chitosan/Antarctic krill protein composite system	20:68	chitosan/Antarctic krill protein composite system	20:68	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	3	23	theme	wet-spinning	477:488	arg1	machine					490:496	a lab-scale wet-spinning machine	465:496	a lab-scale wet-spinning machine	465:496	Then we obtained CS/AKP fiber by wet-spinning on a lab-scale wet-spinning machine.
31604078	2	24	theme	aqueous	351:357	arg1	solution					359:366	aqueous solution	351:366	aqueous solution	351:366	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	5	25	theme	hydrogen	714:721	arg1	bond					723:726	the hydrogen bond	710:726	the hydrogen bond formed between CS and AKP	710:752	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	5	25	theme	hydrogen	714:721	arg1	strength					664:671	the increased tensile strength	642:671	the increased tensile strength of the blended fibers	642:693	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	6	26	theme	curve	859:863	arg1	fitting					865:871	curve fitting	859:871	curve fitting	859:871	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	2	27	theme	acetic	312:317	arg1	acid					319:322	2 wt% glacial acetic acid	298:322	2 wt% glacial acetic acid	298:322	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	2	28	dep	solvent	289:295	arg1	solution					359:366	aqueous solution	351:366	aqueous solution	351:366	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	1	29	theme	tensile	191:197	arg1	strength					199:206	the tensile strength	187:206	the tensile strength of chitosan (CS)	187:223	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	0	30	theme	protein	45:51	arg1	system					63:68	chitosan/Antarctic krill protein composite system	20:68	chitosan/Antarctic krill protein composite system	20:68	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	7	31	from	factor	984:989	arg1	growth					925:930	crystalline growth	913:930	crystalline growth of CS along the fiber axis	913:957	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	7	32	theme	fiber	948:952	arg1	axis					954:957	the fiber axis	944:957	the fiber axis	944:957	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	1	33	theme	strength	199:206	arg1	interpretation					169:182	the interpretation	165:182	the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP)	165:265	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	2	34	theme	ethylene	335:342	arg1	glycol					344:349	2 wt% ethylene glycol	329:349	2 wt% ethylene glycol	329:349	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	0	35	theme	krill	39:43	arg1	system					63:68	chitosan/Antarctic krill protein composite system	20:68	chitosan/Antarctic krill protein composite system	20:68	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	2	36	theme	CS/AKP	389:394	arg1	solution					406:413	a stable CS/AKP composite solution	380:413	a stable CS/AKP composite solution	380:413	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	0	37	from	Study	71:75	arg1	enhancement					97:107	enhancement	97:107	enhancement	97:107	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	0	37	from	Study	71:75	arg1	construction					80:91	construction	80:91	construction	80:91	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	7	38	theme	crystalline	913:923	arg1	growth					925:930	crystalline growth	913:930	crystalline growth of CS along the fiber axis	913:957	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	2	39	theme	%	333:333	arg1	glycol					344:349	2 wt% ethylene glycol	329:349	2 wt% ethylene glycol	329:349	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	2	40	theme	stable	382:387	arg1	solution					406:413	a stable CS/AKP composite solution	380:413	a stable CS/AKP composite solution	380:413	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	4	41	from	2.53 cN/dtex	541:552	arg1	state					565:569	the dry state	557:569	the dry state	557:569	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	4	42	theme	CS	617:618	arg1	fibers					620:625	the CS fibers	613:625	the CS fibers	613:625	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	0	43	theme	composite	53:61	arg1	system					63:68	chitosan/Antarctic krill protein composite system	20:68	chitosan/Antarctic krill protein composite system	20:68	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	7	44	from	molecules	1013:1021	arg1	fibers					1034:1039	blended fibers	1026:1039	blended fibers	1026:1039	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	3	45	theme	CS/AKP	433:438	arg1	fiber					440:444	CS/AKP fiber	433:444	CS/AKP fiber	433:444	Then we obtained CS/AKP fiber by wet-spinning on a lab-scale wet-spinning machine.
31604078	6	46	dep	transform	840:848	arg1	infrared					850:857	infrared	850:857	transform infrared curve fitting	840:871	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	5	47	theme	tensile	656:662	arg1	bond					723:726	the hydrogen bond	710:726	the hydrogen bond formed between CS and AKP	710:752	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	5	47	theme	tensile	656:662	arg1	strength					664:671	the increased tensile strength	642:671	the increased tensile strength of the blended fibers	642:693	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	2	48	theme	mixed	283:287	arg1	solvent					289:295	a new mixed solvent	277:295	a new mixed solvent	277:295	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	1	49	theme	chitosan	211:218	arg1	strength					199:206	the tensile strength	187:206	the tensile strength of chitosan (CS)	187:223	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	7	50	theme	CS	935:936	arg1	growth					925:930	crystalline growth	913:930	crystalline growth of CS along the fiber axis	913:957	The hydrogen bond probably benefits on crystalline growth of CS along the fiber axis and is the main drivable factor in removing the water molecules in blended fibers.
31604078	2	51	theme	new	279:281	arg1	solvent					289:295	a new mixed solvent	277:295	a new mixed solvent	277:295	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
31604078	4	52	theme	fibers	531:536	arg1	2.53 cN/dtex					541:552	2.53 cN/dtex	541:552	2.53 cN/dtex	541:552	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	4	52	theme	fibers	531:536	arg1	strength					511:518	The tensile strength	499:518	The tensile strength of blended fibers	499:536	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	5	53	theme	increased	646:654	arg1	bond					723:726	the hydrogen bond	710:726	the hydrogen bond formed between CS and AKP	710:752	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	5	53	theme	increased	646:654	arg1	strength					664:671	the increased tensile strength	642:671	the increased tensile strength of the blended fibers	642:693	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	0	54	dep	bonding	9:15	arg1	Study					71:75	Study	71:75	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.	0:118	Hydrogen bonding in chitosan/Antarctic krill protein composite system: Study on construction and enhancement mechanism.
31604078	4	55	theme	dry	561:563	arg1	state					565:569	the dry state	557:569	the dry state	557:569	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	1	56	theme	work	136:139	arg1	main					124:127	main	124:127	main	124:127	The main of the work concerns the reason for the interpretation of the tensile strength of chitosan (CS) enhanced by Antarctic krill protein (AKP).
31604078	4	57	theme	blended	523:529	arg1	fibers					531:536	blended fibers	523:536	blended fibers	523:536	The tensile strength of blended fibers is 2.53 cN/dtex in the dry state, which is improved by 11.9% compared with the CS fibers.
31604078	6	58	theme	CS/AKP	815:820	arg1	system					822:827	CS/AKP system	815:827	CS/AKP system	815:827	Specifically, illustrate the restructured hydrogen bonds in CS/AKP system in Fourier transform infrared curve fitting.
31604078	5	59	theme	blended	680:686	arg1	fibers					688:693	the blended fibers	676:693	the blended fibers	676:693	We found that the increased tensile strength of the blended fibers is relative to the hydrogen bond formed between CS and AKP.
31604078	2	60	theme	glacial	304:310	arg1	acid					319:322	2 wt% glacial acetic acid	298:322	2 wt% glacial acetic acid	298:322	We found a new mixed solvent, 2 wt% glacial acetic acid, and 2 wt% ethylene glycol aqueous solution, to prepare a stable CS/AKP composite solution.
30528990	0	0	theme	Tridax	78:83	arg1	procumbens					85:94	Tridax procumbens	78:94	Tridax procumbens	78:94	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	8	1	theme	treated	1002:1008	arg1	TPFs					1010:1013	alkali treated TPFs	995:1013	alkali treated TPFs	995:1013	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	1	2	theme	natural	137:143	arg1	fibers					156:161	natural cellulosic fibers	137:161	natural cellulosic fibers extracted from Tridax procumbens plants	137:201	The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants.
30528990	8	3	theme	alkali	995:1000	arg1	TPFs					1010:1013	alkali treated TPFs	995:1013	alkali treated TPFs	995:1013	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	7	4	theme	properties	928:937	arg1	analysis					885:892	the analysis	881:892	the analysis of the fiber diameter and tensile properties	881:937	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	1	5	theme	cellulosic	145:154	arg1	fibers					156:161	natural cellulosic fibers	137:161	natural cellulosic fibers extracted from Tridax procumbens plants	137:201	The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants.
30528990	7	6	theme	Weibull	835:841	arg1	analysis					856:863	The Weibull distribution analysis	831:863	The Weibull distribution analysis	831:863	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	5	7	theme	surface	687:693	arg1	characteristics					705:719	surface roughness characteristics	687:719	surface roughness characteristics	687:719	This enhanced the thermal stability, tensile strength, crystallinity, and surface roughness characteristics.
30528990	7	8	theme	distribution	843:854	arg1	analysis					856:863	The Weibull distribution analysis	831:863	The Weibull distribution analysis	831:863	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	7	9	dep	diameter	907:914	arg1	the					897:899	the	897:899	the	897:899	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	5	10	theme	roughness	695:703	arg1	characteristics					705:719	surface roughness characteristics	687:719	surface roughness characteristics	687:719	This enhanced the thermal stability, tensile strength, crystallinity, and surface roughness characteristics.
30528990	5	11	theme	thermal	631:637	arg1	stability					639:647	the thermal stability	627:647	the thermal stability	627:647	This enhanced the thermal stability, tensile strength, crystallinity, and surface roughness characteristics.
30528990	7	12	theme	tensile	920:926	arg1	properties					928:937	tensile properties	920:937	tensile properties	920:937	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	4	13	with	content	531:537	arg1	reduction					546:554	a reduction	544:554	a reduction in hemicellulose, lignin, and wax upon alkali treatment	544:610	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	4	14	from	reduction	546:554	arg1	hemicellulose					559:571	hemicellulose	559:571	hemicellulose	559:571	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	4	14	from	reduction	546:554	arg1	lignin					574:579	lignin	574:579	lignin	574:579	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	4	14	from	reduction	546:554	arg1	wax					586:588	wax	586:588	wax	586:588	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	4	15	theme	test	469:472	arg1	results					474:480	The test results	465:480	The test results	465:480	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	6	16	theme	treated	760:766	arg1	TPFs					768:771	treated TPFs	760:771	treated TPFs which prove its better wettability with the liquid phase	760:828	The contact angle was also lesser for treated TPFs which prove its better wettability with the liquid phase.
30528990	3	17	theme	Tridax	432:437	arg1	fibers					450:455	Tridax procumbens fibers	432:455	Tridax procumbens fibers (TPFs)	432:462	The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and alkali treated Tridax procumbens fibers (TPFs).
30528990	3	17	theme	Tridax	432:437	arg1	TPFs					458:461	TPFs	458:461	TPFs	458:461	The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and alkali treated Tridax procumbens fibers (TPFs).
30528990	8	18	theme	TPFs	1010:1013	arg1	properties					981:990	the properties	977:990	the properties of alkali treated TPFs	977:1013	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	7	19	theme	diameter	907:914	arg1	analysis					885:892	the analysis	881:892	the analysis of the fiber diameter and tensile properties	881:937	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	6	20	theme	better	789:794	arg1	wettability					796:806	its better wettability	785:806	its better wettability	785:806	The contact angle was also lesser for treated TPFs which prove its better wettability with the liquid phase.
30528990	1	21	theme	Tridax	178:183	arg1	plants					196:201	Tridax procumbens plants	178:201	Tridax procumbens plants	178:201	The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants.
30528990	4	22	theme	cellulose	521:529	arg1	content					531:537	cellulose content	521:537	cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment	521:610	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	8	23	theme	polymer	1062:1068	arg1	composites					1070:1079	high-performance polymer composites	1045:1079	high-performance polymer composites	1045:1079	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	8	24	theme	considerable	949:960	arg1	worth					1024:1028	worth	1024:1028	worth for developing high-performance polymer composites	1024:1079	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	8	24	theme	considerable	949:960	arg1	improvement					962:972	the considerable improvement	945:972	the considerable improvement in the properties of alkali treated TPFs	945:1013	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	4	25	theme	alkali	595:600	arg1	treatment					602:610	alkali treatment	595:610	alkali treatment	595:610	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	3	26	theme	surface	366:372	arg1	characteristics					374:388	surface characteristics	366:388	surface characteristics	366:388	The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and alkali treated Tridax procumbens fibers (TPFs).
30528990	8	27	theme	high-performance	1045:1060	arg1	composites					1070:1079	high-performance polymer composites	1045:1079	high-performance polymer composites	1045:1079	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	3	28	theme	procumbens	439:448	arg1	fibers					450:455	Tridax procumbens fibers	432:455	Tridax procumbens fibers (TPFs)	432:462	The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and alkali treated Tridax procumbens fibers (TPFs).
30528990	3	28	theme	procumbens	439:448	arg1	TPFs					458:461	TPFs	458:461	TPFs	458:461	The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and alkali treated Tridax procumbens fibers (TPFs).
30528990	0	29	theme	alkali	28:33	arg1	Characterization					0:15	Characterization	0:15	Characterization of raw and alkali	0:33	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	4	30	from	increase	509:516	arg1	content					531:537	cellulose content	521:537	cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment	521:610	The test results conclude that there was an increase in cellulose content with a reduction in hemicellulose, lignin, and wax upon alkali treatment.
30528990	0	31	theme	natural	47:53	arg1	fibers					66:71	new natural cellulosic fibers	43:71	new natural cellulosic fibers from Tridax procumbens	43:94	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	6	32	theme	liquid	817:822	arg1	phase					824:828	the liquid phase	813:828	the liquid phase	813:828	The contact angle was also lesser for treated TPFs which prove its better wettability with the liquid phase.
30528990	8	33	from	improvement	962:972	arg1	properties					981:990	the properties	977:990	the properties of alkali treated TPFs	977:1013	Thus the considerable improvement in the properties of alkali treated TPFs would be worth for developing high-performance polymer composites.
30528990	0	34	theme	new	43:45	arg1	fibers					66:71	new natural cellulosic fibers	43:71	new natural cellulosic fibers from Tridax procumbens	43:94	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	2	35	theme	obtained	208:215	arg1	fibers					217:222	The obtained fibers	204:222	The obtained fibers	204:222	The obtained fibers were alkali treated for their effective usage as reinforcement in composites.
30528990	6	36	theme	contact	726:732	arg1	angle					734:738	The contact angle	722:738	The contact angle	722:738	The contact angle was also lesser for treated TPFs which prove its better wettability with the liquid phase.
30528990	6	36	theme	contact	726:732	arg1	lesser					749:754	lesser	749:754	lesser	749:754	The contact angle was also lesser for treated TPFs which prove its better wettability with the liquid phase.
30528990	5	37	theme	tensile	650:656	arg1	strength					658:665	tensile strength	650:665	tensile strength	650:665	This enhanced the thermal stability, tensile strength, crystallinity, and surface roughness characteristics.
30528990	7	38	theme	fiber	901:905	arg1	diameter					907:914	fiber diameter	901:914	fiber diameter	901:914	The Weibull distribution analysis was adopted for the analysis of the fiber diameter and tensile properties.
30528990	2	39	theme	effective	254:262	arg1	usage					264:268	their effective usage	248:268	their effective usage as reinforcement in composites	248:299	The obtained fibers were alkali treated for their effective usage as reinforcement in composites.
30528990	1	40	theme	study	113:117	arg1	aim					101:103	The aim	97:103	The aim of this study	97:117	The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants.
30528990	2	41	from	usage	264:268	arg1	composites					290:299	composites	290:299	composites	290:299	The obtained fibers were alkali treated for their effective usage as reinforcement in composites.
30528990	0	42	from	procumbens	85:94	arg1	fibers					66:71	new natural cellulosic fibers	43:71	new natural cellulosic fibers from Tridax procumbens	43:94	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	1	43	theme	procumbens	185:194	arg1	plants					196:201	Tridax procumbens plants	178:201	Tridax procumbens plants	178:201	The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants.
30528990	2	44	dep	treated	236:242	arg1	alkali					229:234	alkali	229:234	alkali	229:234	The obtained fibers were alkali treated for their effective usage as reinforcement in composites.
30528990	0	45	theme	cellulosic	55:64	arg1	fibers					66:71	new natural cellulosic fibers	43:71	new natural cellulosic fibers from Tridax procumbens	43:94	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
30528990	0	46	theme	raw	20:22	arg1	Characterization					0:15	Characterization	0:15	Characterization of raw and alkali	0:33	Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.
31931422	6	0	theme	respective	704:713	arg1	compositions					715:726	their respective compositions	698:726	their respective compositions	698:726	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	0	1	theme	gums	81:84	arg1	addition					62:69	the addition	58:69	the addition of Acacia gums	58:84	Improvement of the foamability of sparkling base wines by the addition of Acacia gums.
31931422	5	2	theme	fractions	463:471	arg1	addition					378:385	the addition	374:385	the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions	374:471	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	0	3	theme	Acacia	74:79	arg1	gums					81:84	Acacia gums	74:84	Acacia gums	74:84	Improvement of the foamability of sparkling base wines by the addition of Acacia gums.
31931422	4	4	from	additive	337:344	arg1	wine					349:352	wine	349:352	wine	349:352	Acacia gum can be used as additive in wine.
31931422	6	5	theme	gum	603:605	arg1	fraction					607:614	the gum fraction	599:614	the gum fraction	599:614	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	5	6	theme	base	516:519	arg1	wines					521:525	different base wines	506:525	different base wines treated with bentonite	506:548	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	7	theme	different	506:514	arg1	wines					521:525	different base wines	506:525	different base wines treated with bentonite	506:548	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	8	theme	wines	521:525	arg1	foamability					491:501	the foamability	487:501	the foamability of different base wines treated with bentonite	487:548	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	9	theme	AsenG	457:461	arg1	fractions					463:471	different AsenG fractions	447:471	different AsenG fractions	447:471	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	1	10	theme	major	142:146	arg1	attributes					148:157	the major attributes	138:157	the major attributes	138:157	In sparkling wine, foam characteristics are one of the major attributes.
31931422	4	11	theme	Acacia	311:316	arg1	additive					337:344	additive	337:344	additive	337:344	Acacia gum can be used as additive in wine.
31931422	4	11	theme	Acacia	311:316	arg1	gum					318:320	Acacia gum	311:320	Acacia gum	311:320	Acacia gum can be used as additive in wine.
31931422	7	12	from	foamability	783:793	arg1	wines					880:884	five and seven out of eight base wines	847:884	five and seven out of eight base wines treated with bentonite	847:907	AsenG and AseyG increase the foamability (by Mosalux - sparging procedure), respectively, in five and seven out of eight base wines treated with bentonite.
31931422	1	13	theme	attributes	148:157	arg1	attributes					148:157	the major attributes	138:157	the major attributes	138:157	In sparkling wine, foam characteristics are one of the major attributes.
31931422	1	13	theme	attributes	148:157	arg1	one					131:133	one	131:133	one	131:133	In sparkling wine, foam characteristics are one of the major attributes.
31931422	0	14	theme	foamability	19:29	arg1	Improvement					0:10	Improvement	0:10	Improvement of the foamability of sparkling base	0:47	Improvement of the foamability of sparkling base wines by the addition of Acacia gums.
31931422	7	15	theme	sparging	809:816	arg1	procedure					818:826	sparging procedure	809:826	sparging procedure	809:826	AsenG and AseyG increase the foamability (by Mosalux - sparging procedure), respectively, in five and seven out of eight base wines treated with bentonite.
31931422	5	16	theme	Acacia	390:395	arg1	AsenG					410:414	AsenG	410:414	AsenG	410:414	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	16	theme	Acacia	390:395	arg1	gum					405:407	Acacia senegal gum	390:407	Acacia senegal gum (AsenG)	390:415	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	17	theme	senegal	397:403	arg1	AsenG					410:414	AsenG	410:414	AsenG	410:414	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	17	theme	senegal	397:403	arg1	gum					405:407	Acacia senegal gum	390:407	Acacia senegal gum (AsenG)	390:415	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	0	18	theme	sparkling	34:42	arg1	base					44:47	sparkling base	34:47	sparkling base	34:47	Improvement of the foamability of sparkling base wines by the addition of Acacia gums.
31931422	5	19	theme	gum	405:407	arg1	addition					378:385	the addition	374:385	the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions	374:471	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	3	20	theme	base	231:234	arg1	wine					236:239	the base wine	227:239	the base wine	227:239	Bentonite is added to the base wine to facilitate the riddling process, but causes a loss of foamability.
31931422	6	21	theme	parameters	742:751	arg1	aspects					687:693	some aspects	682:693	some aspects of their respective compositions and molecular parameters	682:751	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	6	22	dep	gum	592:594	arg1	the					588:590	the	588:590	the	588:590	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	6	22	dep	gum	592:594	arg1	treatment					616:624	treatment	616:624	treatment	616:624	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	7	23	theme	base	875:878	arg1	wines					880:884	five and seven out of eight base wines	847:884	five and seven out of eight base wines treated with bentonite	847:907	AsenG and AseyG increase the foamability (by Mosalux - sparging procedure), respectively, in five and seven out of eight base wines treated with bentonite.
31931422	3	24	theme	foamability	298:308	arg1	loss					290:293	a loss	288:293	a loss of foamability	288:308	Bentonite is added to the base wine to facilitate the riddling process, but causes a loss of foamability.
31931422	3	25	theme	riddling	259:266	arg1	process					268:274	the riddling process	255:274	the riddling process	255:274	Bentonite is added to the base wine to facilitate the riddling process, but causes a loss of foamability.
31931422	6	26	theme	molecular	732:740	arg1	parameters					742:751	molecular parameters	732:751	molecular parameters	732:751	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	2	27	theme	wine	188:191	arg1	components					193:202	wine components	188:202	wine components	188:202	The foam quality depends on wine components.
31931422	0	28	theme	base	44:47	arg1	foamability					19:29	the foamability	15:29	the foamability of sparkling base	15:47	Improvement of the foamability of sparkling base wines by the addition of Acacia gums.
31931422	6	29	theme	compositions	715:726	arg1	aspects					687:693	some aspects	682:693	some aspects of their respective compositions and molecular parameters	682:751	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	5	30	theme	different	447:455	arg1	fractions					463:471	different AsenG fractions	447:471	different AsenG fractions	447:471	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	4	31	used	used	329:332	arg2	additive					337:344	additive	337:344	additive	337:344	Acacia gum can be used as additive in wine.
31931422	4	31	used	used	329:332	arg2	gum					318:320	Acacia gum	311:320	Acacia gum	311:320	Acacia gum can be used as additive in wine.
31931422	4	32	from	wine	349:352	arg1	additive					337:344	additive	337:344	additive	337:344	Acacia gum can be used as additive in wine.
31931422	4	32	from	wine	349:352	arg1	gum					318:320	Acacia gum	311:320	Acacia gum	311:320	Acacia gum can be used as additive in wine.
31931422	2	33	theme	foam	164:167	arg1	quality					169:175	The foam quality	160:175	The foam quality	160:175	The foam quality depends on wine components.
31931422	5	34	theme	Acacia	418:423	arg1	gum					431:433	Acacia seyal gum	418:433	Acacia seyal gum (AseyG)	418:441	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	34	theme	Acacia	418:423	arg1	AseyG					436:440	AseyG	436:440	AseyG	436:440	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	1	35	theme	foam	106:109	arg1	characteristics					111:125	foam characteristics	106:125	foam characteristics	106:125	In sparkling wine, foam characteristics are one of the major attributes.
31931422	8	36	theme	wines	998:1002	arg1	foamability					977:987	the foamability	973:987	the foamability of these wines	973:1002	Therefore, AsenG and AseyG are potential treatments increasing the foamability of these wines.
31931422	5	37	theme	seyal	425:429	arg1	gum					431:433	Acacia seyal gum	418:433	Acacia seyal gum (AseyG)	418:441	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	5	37	theme	seyal	425:429	arg1	AseyG					436:440	AseyG	436:440	AseyG	436:440	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	6	38	attach	linked	672:677	arg1	aspects					687:693	some aspects	682:693	some aspects of their respective compositions and molecular parameters	682:751	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	6	38	attach	linked	672:677	arg2	differences					660:670	these differences	654:670	these differences linked to some aspects of their respective compositions and molecular parameters	654:751	The foamability differs depending on the gum or the gum fraction treatment but also on the wine, being these differences linked to some aspects of their respective compositions and molecular parameters.
31931422	5	39	theme	gum	431:433	arg1	addition					378:385	the addition	374:385	the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions	374:471	We have studied if the addition of Acacia senegal gum (AsenG), Acacia seyal gum (AseyG) and different AsenG fractions could improve the foamability of different base wines treated with bentonite.
31931422	8	40	theme	potential	941:949	arg1	AseyG					931:935	AseyG	931:935	AseyG	931:935	Therefore, AsenG and AseyG are potential treatments increasing the foamability of these wines.
31931422	8	40	theme	potential	941:949	arg1	treatments					951:960	potential treatments	941:960	potential treatments increasing the foamability of these wines	941:1002	Therefore, AsenG and AseyG are potential treatments increasing the foamability of these wines.
31931422	8	40	theme	potential	941:949	arg1	AsenG					921:925	AsenG	921:925	AsenG	921:925	Therefore, AsenG and AseyG are potential treatments increasing the foamability of these wines.
30287381	7	0	theme	EPS	1013:1015	arg1	analysis					1001:1008	SEM and AFM analysis	989:1008	analysis	1001:1008	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	8	1	theme	total	1145:1149	arg1	EPS					1151:1153	total EPS	1145:1153	total EPS secreted by RS20D	1145:1171	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	0	2	theme	immunoregulatory	94:109	arg1	activity					111:118	the immunoregulatory activity	90:118	the immunoregulatory activity	90:118	Exopolysaccharides produced by yogurt-texture improving Lactobacillus plantarum RS20D and the immunoregulatory activity.
30287381	9	3	theme	dairy	1505:1509	arg1	industry					1511:1518	the dairy industry	1501:1518	the dairy industry	1501:1518	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	7	4	theme	AFM	997:999	arg1	analysis					1001:1008	SEM and AFM analysis	989:1008	analysis	1001:1008	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	4	5	theme	2.0:1.5:1	830:838	arg1	ratio					821:825	a molar ratio	813:825	a molar ratio of 2.0:1.5:1	813:838	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	0	6	dep	Lactobacillus	56:68	arg1	plantarum					70:78	plantarum	70:78	plantarum	70:78	Exopolysaccharides produced by yogurt-texture improving Lactobacillus plantarum RS20D and the immunoregulatory activity.
30287381	4	7	theme	molar	815:819	arg1	ratio					821:825	a molar ratio	813:825	a molar ratio of 2.0:1.5:1	813:838	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	8	8	theme	macrophage	1189:1198	arg1	RAW264.7					1200:1207	macrophage RAW264.7	1189:1207	macrophage RAW264.7	1189:1207	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	7	9	theme	SEM	989:991	arg1	analysis					1001:1008	SEM and AFM analysis	989:1008	analysis	1001:1008	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	3	10	theme	RS-r2	596:600	arg1	features					584:591	the structural features	569:591	the structural features of RS-r2	569:600	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	9	11	theme	yogurt	1483:1488	arg1	texture					1490:1496	yogurt texture	1483:1496	yogurt texture	1483:1496	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	3	12	theme	further	537:543	arg1	work					545:548	The further work	533:548	The further work	533:548	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	8	13	theme	mRNA	1311:1314	arg1	level					1316:1320	the mRNA level	1307:1320	the mRNA level	1307:1320	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	3	14	theme	structural	573:582	arg1	features					584:591	the structural features	569:591	the structural features of RS-r2	569:600	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	2	15	theme	Sephadex	444:451	arg1	G-100					453:457	Sephadex G-100	444:457	Sephadex G-100	444:457	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	8	16	dep	In	1119:1120	arg1	vitro					1122:1126	vitro	1122:1126	vitro	1122:1126	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	1	17	attach	isolated	192:199	arg2	RS20D					132:136	The strain RS20D	121:136	The strain RS20D capable of significantly improving yogurt texture	121:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	17	attach	isolated	192:199	arg2	RS20D					290:294	Lactobacillus plantarum RS20D	266:294	Lactobacillus plantarum RS20D	266:294	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	17	attach	isolated	192:199	arg1	products					238:245	traditional fermented vegetable products	206:245	traditional fermented vegetable products	206:245	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	8	18	theme	gene	1258:1261	arg1	expression					1263:1272	the gene expression	1254:1272	the gene expression of pro-inflammatory cytokines	1254:1302	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	1	19	theme	traditional	206:216	arg1	products					238:245	traditional fermented vegetable products	206:245	traditional fermented vegetable products	206:245	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	6	20	theme	degradation	916:926	arg1	250 °C					941:946	250 °C	941:946	250 °C	941:946	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	6	20	theme	degradation	916:926	arg1	temperature					928:938	a high degradation temperature	909:938	a high degradation temperature (250 °C)	909:947	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	2	21	theme	skim	365:368	arg1	milk					370:373	skim milk	365:373	reconstituted skim milk fermentation by RS20D	351:395	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	1	22	theme	fermented	218:226	arg1	products					238:245	traditional fermented vegetable products	206:245	traditional fermented vegetable products	206:245	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	9	23	theme	potential	1369:1377	arg1	source					1379:1384	a potential source	1367:1384	a potential source of immunoregulatory polysaccharide	1367:1419	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	9	23	theme	potential	1369:1377	arg1	RS20D					1352:1356	RS20D	1352:1356	RS20D	1352:1356	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	2	24	theme	reconstituted	351:363	arg1	fermentation					375:386	reconstituted skim milk fermentation	351:386	reconstituted skim milk fermentation by RS20D	351:395	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	2	25	theme	DEAE-Sepharose	419:432	arg1	CL-6B					434:438	DEAE-Sepharose CL-6B	419:438	DEAE-Sepharose CL-6B	419:438	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	2	26	theme	purified	481:488	arg1	fraction					490:497	the purified fraction	477:497	the purified fraction designated as RS-r2	477:517	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	1	27	theme	strain	125:130	arg1	RS20D					290:294	Lactobacillus plantarum RS20D	266:294	Lactobacillus plantarum RS20D	266:294	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	27	theme	strain	125:130	arg1	RS20D					132:136	The strain RS20D	121:136	The strain RS20D capable of significantly improving yogurt texture	121:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	9	28	theme	immunoregulatory	1389:1404	arg1	polysaccharide					1406:1419	immunoregulatory polysaccharide	1389:1419	immunoregulatory polysaccharide	1389:1419	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	8	29	theme	In	1119:1120	arg1	test					1128:1131	In vitro test	1119:1131	In vitro test	1119:1131	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	6	30	theme	high	911:914	arg1	250 °C					941:946	250 °C	941:946	250 °C	941:946	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	6	30	theme	high	911:914	arg1	temperature					928:938	a high degradation temperature	909:938	a high degradation temperature (250 °C)	909:947	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	3	31	theme	FT-IR	606:610	arg1	spectrum					612:619	FT-IR spectrum	606:619	FT-IR spectrum	606:619	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	4	32	theme	acidic	727:732	arg1	heteropolysaccharide					734:753	a novel acidic heteropolysaccharide	719:753	a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1	719:838	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	4	32	theme	acidic	727:732	arg1	RS-r2					709:713	RS-r2	709:713	RS-r2	709:713	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	0	33	theme	Lactobacillus	56:68	arg1	RS20D					80:84	Lactobacillus plantarum RS20D	56:84	Lactobacillus plantarum RS20D	56:84	Exopolysaccharides produced by yogurt-texture improving Lactobacillus plantarum RS20D and the immunoregulatory activity.
30287381	9	34	theme	polysaccharide	1406:1419	arg1	source					1379:1384	a potential source	1367:1384	a potential source of immunoregulatory polysaccharide	1367:1419	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	9	34	theme	polysaccharide	1406:1419	arg1	RS20D					1352:1356	RS20D	1352:1356	RS20D	1352:1356	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	3	35	theme	monosaccharide	632:645	arg1	analysis					659:666	HPSEC and monosaccharide composition analysis	622:666	analysis	659:666	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	1	36	theme	vegetable	228:236	arg1	products					238:245	traditional fermented vegetable products	206:245	traditional fermented vegetable products	206:245	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	8	37	theme	cytokines	1294:1302	arg1	expression					1263:1272	the gene expression	1254:1272	the gene expression of pro-inflammatory cytokines	1254:1302	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	7	38	theme	aqueous	1101:1107	arg1	solution					1109:1116	aqueous solution	1101:1116	aqueous solution	1101:1116	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	3	39	theme	composition	647:657	arg1	analysis					659:666	HPSEC and monosaccharide composition analysis	622:666	analysis	659:666	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	7	40	theme	spherical	1082:1090	arg1	shape					1092:1096	many regular spherical shape	1069:1096	many regular spherical shape in aqueous solution	1069:1116	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	3	41	theme	HPSEC	622:626	arg1	analysis					659:666	HPSEC and monosaccharide composition analysis	622:666	analysis	659:666	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	1	42	theme	capable	138:144	arg1	RS20D					290:294	Lactobacillus plantarum RS20D	266:294	Lactobacillus plantarum RS20D	266:294	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	42	theme	capable	138:144	arg1	RS20D					132:136	The strain RS20D	121:136	The strain RS20D capable of significantly improving yogurt texture	121:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	5	43	theme	molecular	845:853	arg1	1.69 × 106 Da					882:894	1.69 × 106 Da	882:894	1.69 × 106 Da	882:894	The molecular weight was estimated to be 1.69 × 106 Da.
30287381	5	43	theme	molecular	845:853	arg1	weight					855:860	The molecular weight	841:860	The molecular weight	841:860	The molecular weight was estimated to be 1.69 × 106 Da.
30287381	6	44	theme	thermal	970:976	arg1	stability					978:986	its high thermal stability	961:986	its high thermal stability	961:986	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	7	45	theme	chain	1034:1038	arg1	microstructure					1040:1053	chain microstructure	1034:1053	chain microstructure anchored with many regular spherical shape in aqueous solution	1034:1116	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	6	46	contain	had	905:907	arg2	250 °C					941:946	250 °C	941:946	250 °C	941:946	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	6	46	contain	had	905:907	arg2	temperature					928:938	a high degradation temperature	909:938	a high degradation temperature (250 °C)	909:947	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	6	46	contain	had	905:907	arg1	EPS					901:903	The EPS	897:903	The EPS	897:903	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	6	47	theme	high	965:968	arg1	stability					978:986	its high thermal stability	961:986	its high thermal stability	961:986	The EPS had a high degradation temperature (250 °C), suggesting its high thermal stability.
30287381	3	48	dep	elucidate	559:567	arg1	carried					672:678	carried	672:678	was carried out	668:682	The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out.
30287381	7	49	theme	regular	1074:1080	arg1	shape					1092:1096	many regular spherical shape	1069:1096	many regular spherical shape in aqueous solution	1069:1116	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30287381	2	50	theme	total	301:305	arg1	EPS					327:329	EPS	327:329	EPS	327:329	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	2	50	theme	total	301:305	arg1	exopolysaccharides					307:324	The total exopolysaccharides	297:324	The total exopolysaccharides (EPS)	297:330	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	9	51	theme	functional	1445:1454	arg1	RS20D					1352:1356	RS20D	1352:1356	RS20D	1352:1356	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	9	51	theme	functional	1445:1454	arg1	culture					1464:1470	a functional starter culture	1443:1470	a functional starter culture to improve yogurt texture in the dairy industry	1443:1518	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	2	52	theme	milk	370:373	arg1	fermentation					375:386	reconstituted skim milk fermentation	351:386	reconstituted skim milk fermentation by RS20D	351:395	The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained.
30287381	1	53	theme	Lactobacillus	266:278	arg1	RS20D					290:294	Lactobacillus plantarum RS20D	266:294	Lactobacillus plantarum RS20D	266:294	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	53	theme	Lactobacillus	266:278	arg1	RS20D					132:136	The strain RS20D	121:136	The strain RS20D capable of significantly improving yogurt texture	121:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	9	54	theme	Current	1323:1329	arg1	study					1331:1335	Current study	1323:1335	Current study	1323:1335	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	1	55	theme	yogurt	173:178	arg1	texture					180:186	yogurt texture	173:186	yogurt texture	173:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	8	56	theme	pro-inflammatory	1277:1292	arg1	cytokines					1294:1302	pro-inflammatory cytokines	1277:1302	pro-inflammatory cytokines	1277:1302	In vitro test showed that total EPS secreted by RS20D could stimulate macrophage RAW264.7 to release NO significantly and up-regulated the gene expression of pro-inflammatory cytokines at the mRNA level.
30287381	1	57	theme	plantarum	280:288	arg1	RS20D					290:294	Lactobacillus plantarum RS20D	266:294	Lactobacillus plantarum RS20D	266:294	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	1	57	theme	plantarum	280:288	arg1	RS20D					132:136	The strain RS20D	121:136	The strain RS20D capable of significantly improving yogurt texture	121:186	The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D.
30287381	9	58	theme	starter	1456:1462	arg1	RS20D					1352:1356	RS20D	1352:1356	RS20D	1352:1356	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	9	58	theme	starter	1456:1462	arg1	culture					1464:1470	a functional starter culture	1443:1470	a functional starter culture to improve yogurt texture in the dairy industry	1443:1518	Current study suggested that RS20D could be a potential source of immunoregulatory polysaccharide and may be applied as a functional starter culture to improve yogurt texture in the dairy industry.
30287381	4	59	theme	novel	721:725	arg1	heteropolysaccharide					734:753	a novel acidic heteropolysaccharide	719:753	a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1	719:838	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	4	59	theme	novel	721:725	arg1	RS-r2					709:713	RS-r2	709:713	RS-r2	709:713	The results showed that RS-r2 was a novel acidic heteropolysaccharide mainly consisted of glucose, galactose and glucosamine in a molar ratio of 2.0:1.5:1.
30287381	7	60	from	shape	1092:1096	arg1	solution					1109:1116	aqueous solution	1101:1116	aqueous solution	1101:1116	SEM and AFM analysis of EPS further revealed chain microstructure anchored with many regular spherical shape in aqueous solution.
30241850	0	0	theme	mushroom	94:101	arg1	aegerita					85:92	Agrocybe aegerita mushroom	76:101	Agrocybe aegerita mushroom	76:101	Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom.
30241850	8	1	with	type	995:998	arg1	μM					1023:1024	Kiof 1.54 ± 0.15 μM	1006:1024	Kiof 1.54 ± 0.15 μM	1006:1024	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	5	2	theme	monosaccharide	557:570	arg1	composition					572:582	monosaccharide composition	557:582	monosaccharide composition	557:582	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	7	3	theme	5.82 ± 0.57	905:915	arg1	μM					917:918	5.82 ± 0.57 μM	905:918	5.82 ± 0.57 μM	905:918	FG-Aa showed significant inhibitory activity on ARG with IC50potency of 5.82 ± 0.57 μM.
30241850	6	4	theme	α-D-Galp	745:752	arg1	main-chain					754:763	a (1→6)-linked α-D-Galp main-chain	730:763	a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp	730:830	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	6	5	theme	α-L-Fucp	823:830	arg1	end-units					810:818	non-reducing end-units	797:818	non-reducing end-units of α-L-Fucp	797:830	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	4	6	with	precipitation	478:490	arg1	solution					505:512	Fehling solution	497:512	Fehling solution	497:512	The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution.
30241850	8	7	theme	Kiof	1006:1009	arg1	μM					1023:1024	Kiof 1.54 ± 0.15 μM	1006:1024	Kiof 1.54 ± 0.15 μM	1006:1024	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	5	8	theme	NMR	636:638	arg1	spectroscopy					640:651	NMR spectroscopy	636:651	NMR spectroscopy	636:651	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	9	9	theme	inhibitory	1058:1067	arg1	activity					1069:1076	an inhibitory activity	1055:1076	an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1055:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	3	10	from	amazonensis	374:384	arg1	arginase					343:350	arginase	343:350	arginase (ARG) from Leishmania amazonensis	343:384	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	3	10	from	amazonensis	374:384	arg1	ARG					353:355	ARG	353:355	ARG	353:355	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	8	11	theme	inhibition	942:951	arg1	type					995:998	the competitive type	979:998	the competitive type	979:998	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	8	11	theme	inhibition	942:951	arg1	mechanism					925:933	The mechanism	921:933	The mechanism of ARG inhibition by the heterogalactan	921:973	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	4	12	theme	aqueous	423:429	arg1	extraction					431:440	aqueous extraction	423:440	aqueous extraction	423:440	The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution.
30241850	9	13	theme	activity	1069:1076	arg1	This					1027:1030	This	1027:1030	This	1027:1030	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	13	theme	activity	1069:1076	arg1	report					1045:1050	the first report	1035:1050	the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1035:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	6	14	theme	non-reducing	797:808	arg1	end-units					810:818	non-reducing end-units	797:818	non-reducing end-units of α-L-Fucp	797:830	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	3	15	theme	fucogalactan	253:264	arg1	potential					238:246	the potential	234:246	the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita	234:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	1	16	from	Leishmania	136:145	arg1	inhibition					108:117	The inhibition	104:117	The inhibition of arginase from Leishmania	104:145	The inhibition of arginase from Leishmania spp.
30241850	5	17	theme	chemical	519:526	arg1	structure					528:536	Its chemical structure	515:536	Its chemical structure	515:536	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	4	18	with	thawing	466:472	arg1	solution					505:512	Fehling solution	497:512	Fehling solution	497:512	The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution.
30241850	4	19	theme	Fehling	497:503	arg1	solution					505:512	Fehling solution	497:512	Fehling solution	497:512	The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution.
30241850	5	20	theme	partial	607:613	arg1	hydrolysis					620:629	partial acid hydrolysis	607:629	partial acid hydrolysis	607:629	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	6	21	link	-linked	737:743	arg1	main-chain					754:763	a (1→6)-linked α-D-Galp main-chain	730:763	a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp	730:830	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	9	22	theme	arginase	1081:1088	arg1	activity					1069:1076	an inhibitory activity	1055:1076	an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1055:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	5	23	theme	acid	615:618	arg1	hydrolysis					620:629	partial acid hydrolysis	607:629	partial acid hydrolysis	607:629	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	0	24	theme	amazonensis	25:35	arg1	arginase					37:44	Leishmania amazonensis arginase	14:44	Leishmania amazonensis arginase	14:44	Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom.
30241850	9	25	theme	inhibitors	1207:1216	arg1	class					1194:1198	a new class	1188:1198	a new class of ARG inhibitors	1188:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	25	theme	inhibitors	1207:1216	arg1	polysaccharides					1169:1183	further polysaccharides	1161:1183	further polysaccharides	1161:1183	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	6	26	dep	FG-Aa	700:704	arg1	Mw = 13.8 kDa					707:719	Mw = 13.8 kDa	707:719	Mw = 13.8 kDa	707:719	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	7	27	theme	significant	846:856	arg1	activity					869:876	significant inhibitory activity	846:876	significant inhibitory activity	846:876	FG-Aa showed significant inhibitory activity on ARG with IC50potency of 5.82 ± 0.57 μM.
30241850	3	28	theme	medicinal	284:292	arg1	aegerita					312:319	the medicinal mushroom Agrocybe aegerita	280:319	the medicinal mushroom Agrocybe aegerita	280:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	4	29	theme	freeze	459:464	arg1	thawing					466:472	freeze thawing	459:472	freeze thawing	459:472	The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution.
30241850	9	30	theme	new	1190:1192	arg1	class					1194:1198	a new class	1188:1198	a new class of ARG inhibitors	1188:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	30	theme	new	1190:1192	arg1	polysaccharides					1169:1183	further polysaccharides	1161:1183	further polysaccharides	1161:1183	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	0	31	theme	arginase	37:44	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of Leishmania amazonensis arginase by fucogalactan	0:60	Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom.
30241850	8	32	theme	ARG	938:940	arg1	inhibition					942:951	ARG inhibition	938:951	ARG inhibition	938:951	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	8	33	theme	competitive	983:993	arg1	type					995:998	the competitive type	979:998	the competitive type	979:998	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	8	33	theme	competitive	983:993	arg1	mechanism					925:933	The mechanism	921:933	The mechanism of ARG inhibition by the heterogalactan	921:973	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	2	34	theme	promising	168:176	arg1	approach					178:185	a promising approach	166:185	a promising approach to the leishmaniasis treatment	166:216	is considered a promising approach to the leishmaniasis treatment.
30241850	1	35	theme	arginase	122:129	arg1	inhibition					108:117	The inhibition	104:117	The inhibition of arginase from Leishmania	104:145	The inhibition of arginase from Leishmania spp.
30241850	9	36	from	amazonensis	1098:1108	arg1	arginase					1081:1088	arginase	1081:1088	arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1081:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	36	from	amazonensis	1098:1108	arg1	activity					1069:1076	an inhibitory activity	1055:1076	an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1055:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	5	37	theme	methylation	585:595	arg1	analysis					597:604	methylation analysis	585:604	methylation analysis	585:604	Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy.
30241850	3	38	attach	isolated	266:273	arg2	fucogalactan					253:264	a fucogalactan	251:264	a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita	251:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	3	38	attach	isolated	266:273	arg1	aegerita					312:319	the medicinal mushroom Agrocybe aegerita	280:319	the medicinal mushroom Agrocybe aegerita	280:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	3	39	theme	mushroom	294:301	arg1	aegerita					312:319	the medicinal mushroom Agrocybe aegerita	280:319	the medicinal mushroom Agrocybe aegerita	280:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	8	40	theme	1.54 ± 0.15	1011:1021	arg1	μM					1023:1024	Kiof 1.54 ± 0.15 μM	1006:1024	Kiof 1.54 ± 0.15 μM	1006:1024	The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM.
30241850	6	41	theme	-linked	737:743	arg1	main-chain					754:763	a (1→6)-linked α-D-Galp main-chain	730:763	a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp	730:830	The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp.
30241850	3	42	theme	Agrocybe	303:310	arg1	aegerita					312:319	the medicinal mushroom Agrocybe aegerita	280:319	the medicinal mushroom Agrocybe aegerita	280:319	In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis.
30241850	7	43	theme	μM	917:918	arg1	IC50potency					890:900	IC50potency	890:900	IC50potency of 5.82 ± 0.57 μM	890:918	FG-Aa showed significant inhibitory activity on ARG with IC50potency of 5.82 ± 0.57 μM.
30241850	7	44	theme	inhibitory	858:867	arg1	activity					869:876	significant inhibitory activity	846:876	significant inhibitory activity	846:876	FG-Aa showed significant inhibitory activity on ARG with IC50potency of 5.82 ± 0.57 μM.
30241850	0	45	theme	Agrocybe	76:83	arg1	aegerita					85:92	Agrocybe aegerita mushroom	76:101	Agrocybe aegerita mushroom	76:101	Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom.
30241850	2	46	theme	leishmaniasis	194:206	arg1	treatment					208:216	the leishmaniasis treatment	190:216	the leishmaniasis treatment	190:216	is considered a promising approach to the leishmaniasis treatment.
30241850	9	47	from	activity	1069:1076	arg1	amazonensis					1098:1108	L. amazonensis	1095:1108	L. amazonensis	1095:1108	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	48	theme	first	1039:1043	arg1	This					1027:1030	This	1027:1030	This	1027:1030	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	48	theme	first	1039:1043	arg1	report					1045:1050	the first report	1035:1050	the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors	1035:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	49	theme	ARG	1203:1205	arg1	inhibitors					1207:1216	ARG inhibitors	1203:1216	ARG inhibitors	1203:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	50	theme	further	1161:1167	arg1	class					1194:1198	a new class	1188:1198	a new class of ARG inhibitors	1188:1216	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
30241850	9	50	theme	further	1161:1167	arg1	polysaccharides					1169:1183	further polysaccharides	1161:1183	further polysaccharides	1161:1183	This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.
29864536	7	0	theme	rhamnogalacturonan	861:878	arg1	BCWSP					841:845	the BCWSP	837:845	the BCWSP	837:845	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	7	0	theme	rhamnogalacturonan	861:878	arg1	backbone					880:887	a rhamnogalacturonan backbone	859:887	a rhamnogalacturonan backbone with galactan and arabinan side chains	859:926	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	0	1	from	properties	16:25	arg1	seeds					77:81	black cumin seeds	65:81	black cumin seeds	65:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	11	2	theme	BCWSP	1276:1280	arg1	activity					1260:1267	The DPPH radical scavenging activity	1232:1267	The DPPH radical scavenging activity of the BCWSP	1232:1280	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	11	2	theme	BCWSP	1276:1280	arg1	%					1291:1291	63.25%	1286:1291	63.25%	1286:1291	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	6	3	theme	pyranose	771:778	arg1	form					780:783	pyranose form	771:783	pyranose form	771:783	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	3	4	theme	antioxidant	368:378	arg1	properties					380:389	the antioxidant properties	364:389	the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP)	364:442	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	1	5	attach	isolated	140:147	arg2	polysaccharides					119:133	water-soluble polysaccharides	105:133	water-soluble polysaccharides	105:133	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	1	5	attach	isolated	140:147	arg1	seeds					166:170	black cumin seeds	154:170	black cumin seeds	154:170	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	11	6	theme	scavenging	1249:1258	arg1	activity					1260:1267	The DPPH radical scavenging activity	1232:1267	The DPPH radical scavenging activity of the BCWSP	1232:1280	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	11	6	theme	scavenging	1249:1258	arg1	%					1291:1291	63.25%	1286:1291	63.25%	1286:1291	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	7	7	theme	side	916:919	arg1	chains					921:926	arabinan side chains	907:926	arabinan side chains	907:926	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	12	8	theme	chelating	1370:1378	arg1	abilities					1380:1388	chelating abilities	1370:1388	chelating abilities	1370:1388	The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities.
29864536	2	9	theme	X-ray	313:317	arg1	diffraction					319:329	X-ray diffraction	313:329	X-ray diffraction	313:329	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	10	10	theme	tension	1173:1179	arg1	decrease					1153:1160	the decrease	1149:1160	the decrease of surface tension	1149:1179	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	10	11	theme	zeta	1083:1086	arg1	potential					1088:1096	The polysaccharide zeta potential	1064:1096	The polysaccharide zeta potential	1064:1096	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	6	12	theme	acids	761:765	arg1	COO-					746:749	COO-	746:749	COO- of uronic acids and pyranose form	746:783	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	6	13	theme	important	678:686	arg1	absorptions					688:698	two most important absorptions	669:698	two most important absorptions	669:698	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	10	14	theme	surface	1165:1171	arg1	tension					1173:1179	surface tension	1165:1179	surface tension	1165:1179	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	4	15	theme	galacturonic	494:505	arg1	acid					507:510	galacturonic acid	494:510	galacturonic acid (30.20%)	494:519	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	4	15	theme	galacturonic	494:505	arg1	%					518:518	30.20%	513:518	30.20%	513:518	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	6	16	theme	uronic	754:759	arg1	acids					761:765	uronic acids	754:765	uronic acids	754:765	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	2	17	theme	NMR	292:294	arg1	spectroscopy					296:307	NMR spectroscopy	292:307	NMR spectroscopy	292:307	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	13	18	from	additives	1462:1470	arg1	food					1475:1478	food	1475:1478	food	1475:1478	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	13	18	from	additives	1462:1470	arg1	products					1493:1500	non-food products	1484:1500	non-food products	1484:1500	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	5	19	theme	high	597:600	arg1	weight					617:622	high peak molecular weight	597:622	high peak molecular weight	597:622	BCWSP was composed of high peak molecular weight.
29864536	0	20	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of water-soluble polysaccharides from black cumin seeds	0:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	7	21	theme	NMR	800:802	arg1	data					817:820	NMR spectroscopy data	800:820	NMR spectroscopy data	800:820	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	3	22	theme	cumin	400:404	arg1	BCWSP					437:441	BCWSP	437:441	BCWSP	437:441	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	3	22	theme	cumin	400:404	arg1	polysaccharides					420:434	black cumin water-soluble polysaccharides	394:434	black cumin water-soluble polysaccharides (BCWSP)	394:442	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	7	23	with	backbone	880:887	arg1	chains					921:926	arabinan side chains	907:926	arabinan side chains	907:926	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	7	23	with	backbone	880:887	arg1	galactan					894:901	galactan	894:901	galactan	894:901	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	5	24	theme	peak	602:605	arg1	weight					617:622	high peak molecular weight	597:622	high peak molecular weight	597:622	BCWSP was composed of high peak molecular weight.
29864536	7	25	theme	arabinan	907:914	arg1	chains					921:926	arabinan side chains	907:926	arabinan side chains	907:926	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	1	26	theme	present	91:97	arg1	work					99:102	the present work	87:102	the present work	87:102	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	7	27	theme	spectroscopy	804:815	arg1	data					817:820	NMR spectroscopy data	800:820	NMR spectroscopy data	800:820	NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains.
29864536	3	28	theme	water-soluble	406:418	arg1	BCWSP					437:441	BCWSP	437:441	BCWSP	437:441	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	3	28	theme	water-soluble	406:418	arg1	polysaccharides					420:434	black cumin water-soluble polysaccharides	394:434	black cumin water-soluble polysaccharides (BCWSP)	394:442	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	5	29	theme	molecular	607:615	arg1	weight					617:622	high peak molecular weight	597:622	high peak molecular weight	597:622	BCWSP was composed of high peak molecular weight.
29864536	12	30	theme	moderate	1325:1332	arg1	bleaching					1356:1364	moderate reductive, β carotene bleaching	1325:1364	moderate reductive, β carotene bleaching	1325:1364	The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities.
29864536	0	31	theme	water-soluble	30:42	arg1	polysaccharides					44:58	water-soluble polysaccharides	30:58	water-soluble polysaccharides from black cumin seeds	30:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	11	32	from	1 mg/mL	1296:1302	arg1	activity					1260:1267	The DPPH radical scavenging activity	1232:1267	The DPPH radical scavenging activity of the BCWSP	1232:1280	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	11	32	from	1 mg/mL	1296:1302	arg1	%					1291:1291	63.25%	1286:1291	63.25%	1286:1291	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	8	33	theme	BCWSP	985:989	arg1	behavior					973:980	the semi-crystalline behavior	952:980	the semi-crystalline behavior of BCWSP	952:989	X-ray pattern revealed the semi-crystalline behavior of BCWSP.
29864536	12	34	dep	reductive	1334:1342	arg1	β					1345:1345	β	1345:1345	β	1345:1345	The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities.
29864536	3	35	theme	black	394:398	arg1	BCWSP					437:441	BCWSP	437:441	BCWSP	437:441	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	3	35	theme	black	394:398	arg1	polysaccharides					420:434	black cumin water-soluble polysaccharides	394:434	black cumin water-soluble polysaccharides (BCWSP)	394:442	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	1	36	theme	water-soluble	105:117	arg1	polysaccharides					119:133	water-soluble polysaccharides	105:133	water-soluble polysaccharides	105:133	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	4	37	theme	neutral	551:557	arg1	%					571:571	22.99%	566:571	22.99%	566:571	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	4	37	theme	neutral	551:557	arg1	sugar					559:563	neutral sugar	551:563	neutral sugar (22.99%)	551:572	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	2	38	theme	thermal	261:267	arg1	composition					230:240	their carbohydrate composition	211:240	their carbohydrate composition	211:240	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	2	38	theme	thermal	261:267	arg1	stability					269:277	thermal stability	261:277	thermal stability	261:277	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	0	39	theme	polysaccharides	44:58	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of water-soluble polysaccharides from black cumin seeds	0:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	8	40	theme	semi-crystalline	956:971	arg1	behavior					973:980	the semi-crystalline behavior	952:980	the semi-crystalline behavior of BCWSP	952:989	X-ray pattern revealed the semi-crystalline behavior of BCWSP.
29864536	4	41	theme	glucuronic	522:531	arg1	acid					533:536	glucuronic acid	522:536	glucuronic acid (17.66%)	522:545	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	4	41	theme	glucuronic	522:531	arg1	%					544:544	17.66%	539:544	17.66%	539:544	BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%).
29864536	13	42	theme	alternative	1450:1460	arg1	BCWSP					1427:1431	BCWSP	1427:1431	BCWSP	1427:1431	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	13	42	theme	alternative	1450:1460	arg1	additives					1462:1470	alternative additives	1450:1470	alternative additives in food and non-food products	1450:1500	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	0	43	theme	cumin	71:75	arg1	seeds					77:81	black cumin seeds	65:81	black cumin seeds	65:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	11	44	theme	DPPH	1236:1239	arg1	activity					1260:1267	The DPPH radical scavenging activity	1232:1267	The DPPH radical scavenging activity of the BCWSP	1232:1280	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	11	44	theme	DPPH	1236:1239	arg1	%					1291:1291	63.25%	1286:1291	63.25%	1286:1291	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	0	45	from	seeds	77:81	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of water-soluble polysaccharides from black cumin seeds	0:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	0	45	from	seeds	77:81	arg1	polysaccharides					44:58	water-soluble polysaccharides	30:58	water-soluble polysaccharides from black cumin seeds	30:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	12	46	theme	reductive	1334:1342	arg1	bleaching					1356:1364	moderate reductive, β carotene bleaching	1325:1364	moderate reductive, β carotene bleaching	1325:1364	The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities.
29864536	0	47	theme	black	65:69	arg1	seeds					77:81	black cumin seeds	65:81	black cumin seeds	65:81	Physicochemical properties of water-soluble polysaccharides from black cumin seeds.
29864536	10	48	theme	pH.	1122:1124	arg1	Results					1126:1132	pH. Results	1122:1132	pH. Results	1122:1132	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	11	49	theme	radical	1241:1247	arg1	activity					1260:1267	The DPPH radical scavenging activity	1232:1267	The DPPH radical scavenging activity of the BCWSP	1232:1280	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	11	49	theme	radical	1241:1247	arg1	%					1291:1291	63.25%	1286:1291	63.25%	1286:1291	The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL.
29864536	2	50	theme	molecular	243:251	arg1	composition					230:240	their carbohydrate composition	211:240	their carbohydrate composition	211:240	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	2	50	theme	molecular	243:251	arg1	weight					253:258	molecular weight	243:258	molecular weight	243:258	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	13	51	used	used	1442:1445	arg2	BCWSP					1427:1431	BCWSP	1427:1431	BCWSP	1427:1431	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	13	51	used	used	1442:1445	arg2	additives					1462:1470	alternative additives	1450:1470	alternative additives in food and non-food products	1450:1500	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	6	52	theme	FTIR	629:632	arg1	spectrum					634:641	The FTIR spectrum	625:641	The FTIR spectrum obtained for BCWSP	625:660	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	13	53	theme	non-food	1484:1491	arg1	products					1493:1500	non-food products	1484:1500	non-food products	1484:1500	Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products.
29864536	10	54	theme	polysaccharide	1068:1081	arg1	potential					1088:1096	The polysaccharide zeta potential	1064:1096	The polysaccharide zeta potential	1064:1096	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	10	55	theme	emulsifying	1208:1218	arg1	capacities					1220:1229	foaming and emulsifying capacities	1196:1229	foaming and emulsifying capacities	1196:1229	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	12	56	theme	carotene	1347:1354	arg1	bleaching					1356:1364	moderate reductive, β carotene bleaching	1325:1364	moderate reductive, β carotene bleaching	1325:1364	The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities.
29864536	2	57	theme	carbohydrate	217:228	arg1	composition					230:240	their carbohydrate composition	211:240	their carbohydrate composition	211:240	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	2	57	theme	carbohydrate	217:228	arg1	weight					253:258	molecular weight	243:258	molecular weight	243:258	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	2	57	theme	carbohydrate	217:228	arg1	stability					269:277	thermal stability	261:277	thermal stability	261:277	Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction.
29864536	6	58	theme	form	780:783	arg1	COO-					746:749	COO-	746:749	COO- of uronic acids and pyranose form	746:783	The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm-1, which corresponded to COO- of uronic acids and pyranose form, respectively.
29864536	1	59	theme	black	154:158	arg1	seeds					166:170	black cumin seeds	154:170	black cumin seeds	154:170	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	10	60	theme	foaming	1196:1202	arg1	capacities					1220:1229	foaming and emulsifying capacities	1196:1229	foaming and emulsifying capacities	1196:1229	The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities.
29864536	3	61	theme	polysaccharides	420:434	arg1	surface					336:342	The surface	332:342	The surface	332:342	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	3	61	theme	polysaccharides	420:434	arg1	properties					380:389	the antioxidant properties	364:389	the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP)	364:442	The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated.
29864536	8	62	theme	X-ray	929:933	arg1	pattern					935:941	X-ray pattern	929:941	X-ray pattern	929:941	X-ray pattern revealed the semi-crystalline behavior of BCWSP.
29864536	1	63	theme	cumin	160:164	arg1	seeds					166:170	black cumin seeds	154:170	black cumin seeds	154:170	In the present work, water-soluble polysaccharides were isolated from black cumin seeds.
29864536	9	64	theme	BCWSP	1007:1011	arg1	OHC					1000:1002	OHC	1000:1002	OHC	1000:1002	WHC and OHC of BCWSP were relatively high and varied with temperatures.
29864536	9	64	theme	BCWSP	1007:1011	arg1	WHC					992:994	WHC	992:994	WHC	992:994	WHC and OHC of BCWSP were relatively high and varied with temperatures.
30102529	7	0	theme	quercetin	1023:1031	arg1	bioaccessibility					1033:1048	quercetin bioaccessibility	1023:1048	quercetin bioaccessibility	1023:1048	There was a good correlation between quercetin bioaccessibility and the lipolysis rate.
30102529	1	1	theme	nutraceutical	150:162	arg1	emulsions					164:172	gelled nutraceutical emulsions	143:172	gelled nutraceutical emulsions	143:172	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	5	2	theme	increasing	809:818	arg1	level					831:835	an increasing gellan gum level	806:835	an increasing gellan gum level in the gelled emulsions	806:859	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	3	3	from	behavior	440:447	arg1	tract					512:516	a simulated gastrointestinal tract	483:516	a simulated gastrointestinal tract	483:516	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	7	4	theme	good	998:1001	arg1	correlation					1003:1013	a good correlation	996:1013	a good correlation between quercetin bioaccessibility and the lipolysis rate	996:1071	There was a good correlation between quercetin bioaccessibility and the lipolysis rate.
30102529	8	5	theme	rheological	1210:1220	arg1	properties					1222:1231	different rheological properties	1200:1231	different rheological properties	1200:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	4	6	theme	Fluid	559:563	arg1	emulsions					565:573	Fluid emulsions	559:573	Fluid emulsions containing different gellan gum levels (0-0.2%)	559:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	2	7	theme	Gellan	327:332	arg1	addition					338:345	Gellan gum addition	327:345	Gellan gum addition	327:345	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	8	8	theme	nutraceutical-loaded	1114:1133	arg1	emulsions					1135:1143	nutraceutical-loaded emulsions	1114:1143	nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties	1114:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	4	9	theme	similar	627:633	arg1	protein					635:641	similar protein and lipid hydrolysis rates	627:668	protein	635:641	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	10	contain	containing	575:584	arg2	%					620:620	0-0.2%	615:620	0-0.2%	615:620	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	10	contain	containing	575:584	arg2	levels					607:612	different gellan gum levels	586:612	different gellan gum levels (0-0.2%)	586:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	10	contain	containing	575:584	arg1	emulsions					565:573	Fluid emulsions	559:573	Fluid emulsions containing different gellan gum levels (0-0.2%)	559:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	3	11	theme	emulsions	470:478	arg1	behavior					440:447	The behavior	436:447	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract	436:516	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	3	12	theme	nutraceutical	456:468	arg1	emulsions					470:478	the nutraceutical emulsions	452:478	the nutraceutical emulsions	452:478	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	5	13	theme	gelled	844:849	arg1	emulsions					851:859	the gelled emulsions	840:859	the gelled emulsions	840:859	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	4	14	theme	quercetin	689:697	arg1	bioaccessibility					699:714	similar quercetin bioaccessibility	681:714	similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%)	627:721	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	14	theme	quercetin	689:697	arg1	%					720:720	∼51%	717:720	∼51%	717:720	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	5	15	dep	proteolysis	736:746	arg1	bioaccessibility					774:789	bioaccessibility	774:789	bioaccessibility	774:789	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	0	16	dep	Proteolysis	77:87	arg1	Bioaccessibility					115:130	Bioaccessibility	115:130	Bioaccessibility	115:130	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	2	17	theme	emulsions	411:419	arg1	viscosity					361:369	viscosity	361:369	viscosity	361:369	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	2	17	theme	emulsions	411:419	arg1	strength					378:385	gel strength	374:385	gel strength	374:385	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	0	18	theme	Gastrointestinal	0:15	arg1	Fate					17:20	Gastrointestinal Fate	0:20	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.	0:131	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	6	19	theme	quercetin	958:966	arg1	bioaccessibility					968:983	higher quercetin bioaccessibility	951:983	higher quercetin bioaccessibility	951:983	In comparison to gelled emulsions, fluid emulsions were digested more rapidly and led to higher quercetin bioaccessibility.
30102529	2	20	dep	viscosity	361:369	arg1	the					357:359	the	357:359	the	357:359	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	2	21	theme	gelled	404:409	arg1	emulsions					411:419	the fluid and gelled emulsions	390:419	the fluid and gelled emulsions	390:419	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	0	22	theme	Fluid	25:29	arg1	Emulsions					56:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	1	23	theme	caseinate-stabilized	212:231	arg1	emulsions					233:241	quercetin-loaded caseinate-stabilized emulsions	195:241	quercetin-loaded caseinate-stabilized emulsions	195:241	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	2	24	theme	gum	334:336	arg1	addition					338:345	Gellan gum addition	327:345	Gellan gum addition	327:345	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	8	25	theme	products	1186:1193	arg1	range					1172:1176	a wide range	1165:1176	a wide range of food products with different rheological properties	1165:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	0	26	theme	Gelled	35:40	arg1	Emulsions					56:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	3	27	theme	initial	538:544	arg1	composition					546:556	their initial composition	532:556	their initial composition	532:556	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	6	28	theme	fluid	897:901	arg1	emulsions					903:911	fluid emulsions	897:911	fluid emulsions	897:911	In comparison to gelled emulsions, fluid emulsions were digested more rapidly and led to higher quercetin bioaccessibility.
30102529	1	29	theme	quercetin-loaded	195:210	arg1	emulsions					233:241	quercetin-loaded caseinate-stabilized emulsions	195:241	quercetin-loaded caseinate-stabilized emulsions	195:241	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	4	30	theme	similar	681:687	arg1	bioaccessibility					699:714	similar quercetin bioaccessibility	681:714	similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%)	627:721	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	30	theme	similar	681:687	arg1	%					720:720	∼51%	717:720	∼51%	717:720	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	5	31	theme	gum	827:829	arg1	level					831:835	an increasing gellan gum level	806:835	an increasing gellan gum level in the gelled emulsions	806:859	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	4	32	theme	different	586:594	arg1	levels					607:612	different gellan gum levels	586:612	different gellan gum levels (0-0.2%)	586:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	32	theme	different	586:594	arg1	%					620:620	0-0.2%	615:620	0-0.2%	615:620	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	0	33	from	Impact	67:72	arg1	Lipolysis					90:98	Lipolysis	90:98	Lipolysis	90:98	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	0	33	from	Impact	67:72	arg1	Quercetin					105:113	Quercetin	105:113	Quercetin	105:113	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	0	33	from	Impact	67:72	arg1	Proteolysis					77:87	Proteolysis	77:87	Proteolysis	77:87	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	3	34	theme	simulated	485:493	arg1	tract					512:516	a simulated gastrointestinal tract	483:516	a simulated gastrointestinal tract	483:516	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	0	35	theme	Emulsions	56:64	arg1	Fate					17:20	Gastrointestinal Fate	0:20	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.	0:131	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	1	36	with	acidification	289:301	arg1	glucono-δ-lactone					308:324	glucono-δ-lactone	308:324	glucono-δ-lactone	308:324	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	0	37	theme	Nutraceutical	42:54	arg1	Emulsions					56:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Fluid and Gelled Nutraceutical Emulsions	25:64	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	4	38	theme	gum	603:605	arg1	levels					607:612	different gellan gum levels	586:612	different gellan gum levels (0-0.2%)	586:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	38	theme	gum	603:605	arg1	%					620:620	0-0.2%	615:620	0-0.2%	615:620	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	6	39	theme	higher	951:956	arg1	bioaccessibility					968:983	higher quercetin bioaccessibility	951:983	higher quercetin bioaccessibility	951:983	In comparison to gelled emulsions, fluid emulsions were digested more rapidly and led to higher quercetin bioaccessibility.
30102529	6	40	theme	gelled	879:884	arg1	emulsions					886:894	gelled emulsions	879:894	gelled emulsions	879:894	In comparison to gelled emulsions, fluid emulsions were digested more rapidly and led to higher quercetin bioaccessibility.
30102529	4	41	contain	had	623:625	arg2	rates					664:668	similar protein and lipid hydrolysis rates	627:668	rates	664:668	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	41	contain	had	623:625	arg2	%					720:720	∼51%	717:720	∼51%	717:720	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	41	contain	had	623:625	arg2	bioaccessibility					699:714	similar quercetin bioaccessibility	681:714	similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%)	627:721	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	41	contain	had	623:625	arg2	protein					635:641	similar protein and lipid hydrolysis rates	627:668	protein	635:641	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	41	contain	had	623:625	arg1	emulsions					565:573	Fluid emulsions	559:573	Fluid emulsions containing different gellan gum levels (0-0.2%)	559:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	2	42	theme	gel	374:376	arg1	strength					378:385	gel strength	374:385	gel strength	374:385	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	4	43	theme	gellan	596:601	arg1	levels					607:612	different gellan gum levels	586:612	different gellan gum levels (0-0.2%)	586:621	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	4	43	theme	gellan	596:601	arg1	%					620:620	0-0.2%	615:620	0-0.2%	615:620	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	8	44	theme	different	1200:1208	arg1	properties					1222:1231	different rheological properties	1200:1231	different rheological properties	1200:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	7	45	theme	lipolysis	1058:1066	arg1	rate					1068:1071	the lipolysis rate	1054:1071	the lipolysis rate	1054:1071	There was a good correlation between quercetin bioaccessibility and the lipolysis rate.
30102529	4	46	theme	lipid	647:651	arg1	rates					664:668	similar protein and lipid hydrolysis rates	627:668	rates	664:668	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	5	47	theme	gellan	820:825	arg1	level					831:835	an increasing gellan gum level	806:835	an increasing gellan gum level in the gelled emulsions	806:859	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	1	48	theme	gelled	143:148	arg1	emulsions					164:172	gelled nutraceutical emulsions	143:172	gelled nutraceutical emulsions	143:172	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	8	49	used	used	1157:1160	arg2	emulsions					1135:1143	nutraceutical-loaded emulsions	1114:1143	nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties	1114:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	8	50	theme	food	1181:1184	arg1	products					1186:1193	food products	1181:1193	food products	1181:1193	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	0	51	dep	Fate	17:20	arg1	Impact					67:72	Impact	67:72	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.	0:131	Gastrointestinal Fate of Fluid and Gelled Nutraceutical Emulsions: Impact on Proteolysis, Lipolysis, and Quercetin Bioaccessibility.
30102529	3	52	theme	gastrointestinal	495:510	arg1	tract					512:516	a simulated gastrointestinal tract	483:516	a simulated gastrointestinal tract	483:516	The behavior of the nutraceutical emulsions in a simulated gastrointestinal tract depended upon their initial composition.
30102529	8	53	with	range	1172:1176	arg1	properties					1222:1231	different rheological properties	1200:1231	different rheological properties	1200:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	1	54	theme	gellan	262:267	arg1	gum					269:271	gellan gum	262:271	gellan gum	262:271	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
30102529	5	55	from	level	831:835	arg1	emulsions					851:859	the gelled emulsions	840:859	the gelled emulsions	840:859	Conversely, proteolysis, lipolysis, and quercetin bioaccessibility decreased with an increasing gellan gum level in the gelled emulsions.
30102529	4	56	theme	hydrolysis	653:662	arg1	rates					664:668	similar protein and lipid hydrolysis rates	627:668	rates	664:668	Fluid emulsions containing different gellan gum levels (0-0.2%) had similar protein and lipid hydrolysis rates as well as similar quercetin bioaccessibility (∼51%).
30102529	2	57	theme	fluid	394:398	arg1	emulsions					411:419	the fluid and gelled emulsions	390:419	the fluid and gelled emulsions	390:419	Gellan gum addition increased the viscosity or gel strength of the fluid and gelled emulsions, respectively.
30102529	8	58	theme	wide	1167:1170	arg1	range					1172:1176	a wide range	1165:1176	a wide range of food products with different rheological properties	1165:1231	These findings are useful for designing nutraceutical-loaded emulsions that can be used in a wide range of food products with different rheological properties.
30102529	1	59	theme	gum	269:271	arg1	addition					250:257	the addition	246:257	the addition of gellan gum with or without acidification with glucono-δ-lactone	246:324	Fluid and gelled nutraceutical emulsions were formulated from quercetin-loaded caseinate-stabilized emulsions by the addition of gellan gum with or without acidification with glucono-δ-lactone.
31473318	0	0	theme	structure	91:99	arg1	characteristics					101:115	structure characteristics	91:115	structure characteristics	91:115	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	2	1	theme	similar	454:460	arg1	structure					462:470	the similar structure	450:470	the similar structure with the host Dendrobium officinale polysaccharide (DOP)	450:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	2	2	theme	reaction	591:598	arg1	conditions					600:609	preferred reaction conditions	581:609	preferred reaction conditions with different C/N ratio	581:634	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	1	3	theme	endophytic	168:177	arg1	fungus					179:184	endophytic fungus	168:184	endophytic fungus	168:184	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	3	4	theme	same	689:692	arg1	composition					709:719	same monosaccharide composition	689:719	same monosaccharide composition	689:719	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	2	5	theme	preferred	581:589	arg1	conditions					600:609	preferred reaction conditions	581:609	preferred reaction conditions with different C/N ratio	581:634	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	1	6	theme	fungus	179:184	arg1	concept					157:163	the concept	153:163	the concept of endophytic fungus	153:184	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	1	7	theme	host	255:258	arg1	product					240:246	the same or similar product	220:246	the same or similar product	220:246	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	1	7	theme	host	255:258	arg1	plant					260:264	the host plant	251:264	the host plant	251:264	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	2	8	theme	officinale	497:506	arg1	DOP					524:526	the host Dendrobium officinale polysaccharide (DOP)	477:527	the host Dendrobium officinale polysaccharide (DOP)	477:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	5	9	theme	safe	1007:1010	arg1	polysaccharide					1012:1025	a safe polysaccharide	1005:1025	a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities	1005:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	5	9	theme	safe	1007:1010	arg1	Meanwhile					974:982	Meanwhile	974:982	Meanwhile	974:982	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	5	10	theme	antiglycation	1050:1062	arg1	activities					1085:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	5	11	theme	good	1032:1035	arg1	activities					1085:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	2	12	theme	Dendrobium	486:495	arg1	DOP					524:526	the host Dendrobium officinale polysaccharide (DOP)	477:527	the host Dendrobium officinale polysaccharide (DOP)	477:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	4	13	dep	-β-L-Man-	852:860	arg1	1→					911:912	α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→	866:912	1→	911:912	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	4	13	dep	-β-L-Man-	852:860	arg1	1→					862:863	1→	862:863	1→	862:863	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	4	13	dep	-β-L-Man-	852:860	arg1	→6					919:920	→6	919:920	→6	919:920	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	5	14	with	polysaccharide	1012:1025	arg1	activities					1085:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	2	15	theme	host	481:484	arg1	DOP					524:526	the host Dendrobium officinale polysaccharide (DOP)	477:527	the host Dendrobium officinale polysaccharide (DOP)	477:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	2	16	theme	C/N	626:628	arg1	ratio					630:634	different C/N ratio	616:634	different C/N ratio	616:634	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	2	17	with	structure	462:470	arg1	DOP					524:526	the host Dendrobium officinale polysaccharide (DOP)	477:527	the host Dendrobium officinale polysaccharide (DOP)	477:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	0	18	theme	biological	121:130	arg1	activities					132:141	biological activities	121:141	biological activities	121:141	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	3	19	theme	monosaccharide	694:707	arg1	composition					709:719	same monosaccharide composition	689:719	same monosaccharide composition	689:719	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	2	20	theme	different	616:624	arg1	ratio					630:634	different C/N ratio	616:634	different C/N ratio	616:634	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	6	21	from	receptors	1155:1163	arg1	cells					1183:1187	RAW264.7 cells	1174:1187	RAW264.7 cells	1174:1187	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	3	22	theme	host-plant-associated	746:766	arg1	polysaccharides					768:782	some host-plant-associated polysaccharides	741:782	DOP as well as some host-plant-associated polysaccharides in published literatures	726:807	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	2	23	theme	endophytic	406:415	arg1	FP					439:440	an endophytic fungus polysaccharide FP	403:440	an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP)	403:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	1	24	theme	active	342:347	arg1	product					349:355	the active product	338:355	the active product	338:355	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	1	25	theme	endophytic	190:199	arg1	fungus					201:206	endophytic fungus	190:206	endophytic fungus	190:206	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	0	26	theme	Fungal	0:5	arg1	polysaccharide					7:20	Fungal polysaccharide	0:20	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.	0:142	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	6	27	theme	membrane	1146:1153	arg1	TLR4					1119:1122	TLR4	1119:1122	TLR4	1119:1122	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	6	27	theme	membrane	1146:1153	arg1	TLR2					1110:1113	TLR2	1110:1113	TLR2	1110:1113	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	6	27	theme	membrane	1146:1153	arg1	receptors					1155:1163	the membrane receptors	1142:1163	the membrane receptors of FP on RAW264.7 cells	1142:1187	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	0	28	theme	officinale	51:60	arg1	polysaccharide					62:75	host Dendrobium officinale polysaccharide	35:75	host Dendrobium officinale polysaccharide	35:75	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	3	29	from	polysaccharides	768:782	arg1	literatures					797:807	published literatures	787:807	published literatures	787:807	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	3	30	dep	2.77 ± 0.51 g/L	666:680	arg1	up					660:661	up	660:661	up	660:661	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	4	31	theme	main	814:817	arg1	chain					819:823	The main chain	810:823	The main chain of FP	810:829	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	4	32	dep	α-D-Glc-	961:968	arg1	1→					970:971	1→	970:971	1→	970:971	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	4	33	theme	-β-L-Man-	852:860	arg1	1→					932:933	→3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→	847:933	→3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→	847:933	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	2	34	with	conditions	600:609	arg1	ratio					630:634	different C/N ratio	616:634	different C/N ratio	616:634	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	0	35	theme	similar	22:28	arg1	polysaccharide					7:20	Fungal polysaccharide	0:20	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.	0:142	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	4	36	theme	-α-D-Glc-	882:890	arg1	1→					911:912	α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→	866:912	1→	911:912	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	0	37	theme	Dendrobium	40:49	arg1	polysaccharide					62:75	host Dendrobium officinale polysaccharide	35:75	host Dendrobium officinale polysaccharide	35:75	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	5	38	theme	immunomodulatory	1068:1083	arg1	activities					1085:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	3	39	with	FP	641:642	arg1	yield					654:658	high yield	649:658	high yield	649:658	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	0	40	theme	host	35:38	arg1	polysaccharide					62:75	host Dendrobium officinale polysaccharide	35:75	host Dendrobium officinale polysaccharide	35:75	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	2	41	theme	polysaccharide	508:521	arg1	DOP					524:526	the host Dendrobium officinale polysaccharide (DOP)	477:527	the host Dendrobium officinale polysaccharide (DOP)	477:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	1	42	theme	same	224:227	arg1	product					240:246	the same or similar product	220:246	the same or similar product	220:246	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	1	42	theme	same	224:227	arg1	plant					260:264	the host plant	251:264	the host plant	251:264	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	2	43	theme	polysaccharide	424:437	arg1	FP					439:440	an endophytic fungus polysaccharide FP	403:440	an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP)	403:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	3	44	theme	published	787:795	arg1	literatures					797:807	published literatures	787:807	published literatures	787:807	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	4	45	theme	-β-D-Gal-	901:909	arg1	1→					911:912	α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→	866:912	1→	911:912	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	0	46	with	similar	22:28	arg1	polysaccharide					62:75	host Dendrobium officinale polysaccharide	35:75	host Dendrobium officinale polysaccharide	35:75	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	2	47	theme	fungus	417:422	arg1	FP					439:440	an endophytic fungus polysaccharide FP	403:440	an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP)	403:527	In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio.
31473318	1	48	theme	restrictions	293:304	arg1	rid					282:284	rid	282:284	rid of the restrictions of farmland	282:316	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	4	49	theme	α-D-Glc-	866:873	arg1	1→					911:912	α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→	866:912	1→	911:912	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	4	50	theme	FP	828:829	arg1	chain					819:823	The main chain	810:823	The main chain of FP	810:829	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	5	51	theme	antioxidant	1037:1047	arg1	activities					1085:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	good antioxidant, antiglycation and immunomodulatory activities	1032:1094	Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities.
31473318	6	52	theme	FP	1168:1169	arg1	TLR4					1119:1122	TLR4	1119:1122	TLR4	1119:1122	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	6	52	theme	FP	1168:1169	arg1	TLR2					1110:1113	TLR2	1110:1113	TLR2	1110:1113	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	6	52	theme	FP	1168:1169	arg1	receptors					1155:1163	the membrane receptors	1142:1163	the membrane receptors of FP on RAW264.7 cells	1142:1187	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	6	53	theme	RAW264.7	1174:1181	arg1	cells					1183:1187	RAW264.7 cells	1174:1187	RAW264.7 cells	1174:1187	Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
31473318	1	54	theme	similar	232:238	arg1	product					240:246	the same or similar product	220:246	the same or similar product	220:246	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	1	54	theme	similar	232:238	arg1	plant					260:264	the host plant	251:264	the host plant	251:264	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	3	55	theme	high	649:652	arg1	yield					654:658	high yield	649:658	high yield	649:658	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	0	56	dep	polysaccharide	7:20	arg1	Preparation					78:88	Preparation	78:88	Preparation	78:88	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	0	56	dep	polysaccharide	7:20	arg1	characteristics					101:115	structure characteristics	91:115	structure characteristics	91:115	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	0	56	dep	polysaccharide	7:20	arg1	activities					132:141	biological activities	121:141	biological activities	121:141	Fungal polysaccharide similar with host Dendrobium officinale polysaccharide: Preparation, structure characteristics and biological activities.
31473318	4	57	theme	-β-D-Gal-	922:930	arg1	1→					932:933	→3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→	847:933	→3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→	847:933	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31473318	1	58	theme	farmland	309:316	arg1	restrictions					293:304	the restrictions	289:304	the restrictions of farmland	289:316	Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained.
31473318	3	59	from	DOP	726:728	arg1	literatures					797:807	published literatures	787:807	published literatures	787:807	The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures.
31473318	4	60	theme	side	946:949	arg1	chain					951:955	the side chain	942:955	the side chain	942:955	The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→.
31633693	0	0	theme	Paper	98:102	arg1	Studies					76:82	Contactless Resonant Cavity Dielectric Spectroscopic Studies	23:82	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	4	1	theme	storage	744:750	arg1	history					752:758	storage history	744:758	storage history	744:758	This offers analysts the ability to quickly discriminate between sheeted materials based on composition and storage history.
31633693	5	2	theme	cavity	830:835	arg1	spectroscopy					848:859	contactless resonant cavity dielectric spectroscopy	809:859	contactless resonant cavity dielectric spectroscopy	809:859	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	1	3	theme	arts	179:182	arg1	substrates					184:193	graphic arts substrates	171:193	graphic arts substrates	171:193	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	0	4	theme	Cellulosic	87:96	arg1	Paper					98:102	Cellulosic Paper	87:102	Cellulosic Paper	87:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	2	5	theme	relevant	360:367	arg1	data					369:372	statistically relevant data	346:372	statistically relevant data for unique and rare materials	346:402	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	5	6	theme	fiber	1072:1076	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	2	7	theme	individual	292:301	arg1	sample					303:308	an individual sample	289:308	an individual sample	289:308	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	5	8	theme	relative	969:976	arg1	age					978:980	the relative age	965:980	the relative age of the paper	965:993	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	9	theme	species	930:936	arg1	compositions					938:949	varying fiber species compositions	916:949	varying fiber species compositions	916:949	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	3	10	theme	non-destructive	450:464	arg1	technique					479:487	a non-destructive, contactless technique	448:487	a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations	448:633	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	10	theme	non-destructive	450:464	arg1	spectroscopy					432:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	0	11	theme	Dielectric	51:60	arg1	Spectroscopic					62:74	Contactless Resonant Cavity Dielectric Spectroscopic	23:74	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	0	12	theme	Cavity	44:49	arg1	Spectroscopic					62:74	Contactless Resonant Cavity Dielectric Spectroscopic	23:74	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	5	13	theme	compositions	938:949	arg1	analytes					904:911	paper analytes	898:911	paper analytes of varying fiber species compositions	898:949	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	14	theme	dielectric	837:846	arg1	spectroscopy					848:859	contactless resonant cavity dielectric spectroscopy	809:859	contactless resonant cavity dielectric spectroscopy	809:859	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	2	15	theme	rare	389:392	arg1	materials					394:402	unique and rare materials	378:402	unique and rare materials	378:402	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	3	16	theme	statistical	607:617	arg1	interpretations					619:633	statistical interpretations	607:633	statistical interpretations	607:633	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	0	17	theme	Contactless	23:33	arg1	Spectroscopic					62:74	Contactless Resonant Cavity Dielectric Spectroscopic	23:74	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	5	18	used	used	868:871	arg2	spectroscopy					848:859	contactless resonant cavity dielectric spectroscopy	809:859	contactless resonant cavity dielectric spectroscopy	809:859	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	1	19	theme	current	109:115	arg1	ex					207:208	ex	207:208	ex	207:208	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	1	19	theme	current	109:115	arg1	techniques					128:137	The current analytical techniques	105:137	The current analytical techniques for characterizing printing and graphic arts substrates	105:193	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	5	20	theme	paper	898:902	arg1	analytes					904:911	paper analytes	898:911	paper analytes of varying fiber species compositions	898:949	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	4	21	dep	offers	641:646	arg1	analysts					648:655	analysts	648:655	offers analysts the ability to quickly discriminate between sheeted materials based on composition and storage history	641:758	This offers analysts the ability to quickly discriminate between sheeted materials based on composition and storage history.
31633693	1	22	theme	analytical	117:126	arg1	ex					207:208	ex	207:208	ex	207:208	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	1	22	theme	analytical	117:126	arg1	techniques					128:137	The current analytical techniques	105:137	The current analytical techniques for characterizing printing and graphic arts substrates	105:193	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	3	23	theme	sheeted	542:548	arg1	material					550:557	a sheeted material	540:557	a sheeted material	540:557	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	24	theme	dielectric	421:430	arg1	technique					479:487	a non-destructive, contactless technique	448:487	a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations	448:633	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	24	theme	dielectric	421:430	arg1	spectroscopy					432:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	5	25	theme	methodology	769:779	arg1	article					781:787	this methodology article	764:787	this methodology article	764:787	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	26	theme	varying	916:922	arg1	compositions					938:949	varying fiber species compositions	916:949	varying fiber species compositions	916:949	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	1	27	dep	ex	207:208	arg1	situ					210:213	situ	210:213	situ	210:213	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	4	28	theme	sheeted	701:707	arg1	materials					709:717	sheeted materials	701:717	sheeted materials based on composition and storage history	701:758	This offers analysts the ability to quickly discriminate between sheeted materials based on composition and storage history.
31633693	0	29	theme	Resonant	35:42	arg1	Spectroscopic					62:74	Contactless Resonant Cavity Dielectric Spectroscopic	23:74	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	5	30	theme	recycled	1063:1070	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	31	theme	fiber	924:928	arg1	compositions					938:949	varying fiber species compositions	916:949	varying fiber species compositions	916:949	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	0	32	theme	Spectroscopic	62:74	arg1	Studies					76:82	Contactless Resonant Cavity Dielectric Spectroscopic Studies	23:82	Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper	23:102	Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper.
31633693	5	33	theme	resonant	821:828	arg1	spectroscopy					848:859	contactless resonant cavity dielectric spectroscopy	809:859	contactless resonant cavity dielectric spectroscopy	809:859	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	3	34	dep	non-destructive	450:464	arg1	contactless					467:477	contactless	467:477	contactless	467:477	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	35	theme	cavity	414:419	arg1	technique					479:487	a non-destructive, contactless technique	448:487	a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations	448:633	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	35	theme	cavity	414:419	arg1	spectroscopy					432:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	5	36	theme	content	1078:1084	arg1	amount					1027:1032	the amount	1023:1032	the amount of post-consumer waste (PCW) recycled fiber content	1023:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	36	theme	content	1078:1084	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	37	theme	PCW	1058:1060	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	38	theme	manufactured	1089:1100	arg1	paper					1109:1113	manufactured office paper	1089:1113	manufactured office paper	1089:1113	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	39	theme	post-consumer	1037:1049	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	40	theme	paper	989:993	arg1	age					978:980	the relative age	965:980	the relative age of the paper	965:993	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	41	theme	office	1102:1107	arg1	paper					1109:1113	manufactured office paper	1089:1113	manufactured office paper	1089:1113	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	5	42	theme	waste	1051:1055	arg1	content					1078:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	post-consumer waste (PCW) recycled fiber content	1037:1084	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	1	43	theme	graphic	171:177	arg1	substrates					184:193	graphic arts substrates	171:193	graphic arts substrates	171:193	The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive.
31633693	5	44	theme	contactless	809:819	arg1	spectroscopy					848:859	contactless resonant cavity dielectric spectroscopy	809:859	contactless resonant cavity dielectric spectroscopy	809:859	In this methodology article, we demonstrate how contactless resonant cavity dielectric spectroscopy may be used to differentiate between paper analytes of varying fiber species compositions, to determine the relative age of the paper, and to detect and quantify the amount of post-consumer waste (PCW) recycled fiber content in manufactured office paper.
31633693	2	45	theme	unique	378:383	arg1	materials					394:402	unique and rare materials	378:402	unique and rare materials	378:402	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	3	46	theme	Resonant	405:412	arg1	technique					479:487	a non-destructive, contactless technique	448:487	a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations	448:633	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	3	46	theme	Resonant	405:412	arg1	spectroscopy					432:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy	405:443	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31633693	2	47	theme	data	258:261	arg1	amount					248:253	the amount	244:253	the amount of data that can be obtained from an individual sample	244:308	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	2	47	theme	data	258:261	arg1	data					258:261	data	258:261	data that can be obtained from an individual sample	258:308	This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically relevant data for unique and rare materials.
31633693	3	48	theme	material	550:557	arg1	sides					531:535	both sides	526:535	both sides of a sheeted material	526:557	Resonant cavity dielectric spectroscopy is a non-destructive, contactless technique which can simultaneously interrogate both sides of a sheeted material and provide measurements which are suitable for statistical interpretations.
31515898	0	0	theme	prawn	94:98	arg1	rosenbergii					114:124	the freshwater prawn Macrobrachium rosenbergii	79:124	the freshwater prawn Macrobrachium rosenbergii	79:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	5	1	theme	synthetic	907:915	arg1	useful					946:951	useful	946:951	useful	946:951	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	5	1	theme	synthetic	907:915	arg1	strategy					917:924	the synthetic strategy	903:924	the synthetic strategy shown here	903:935	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	0	2	theme	freshwater	83:92	arg1	rosenbergii					114:124	the freshwater prawn Macrobrachium rosenbergii	79:124	the freshwater prawn Macrobrachium rosenbergii	79:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	0	3	from	synthesis	9:17	arg1	rosenbergii					114:124	the freshwater prawn Macrobrachium rosenbergii	79:124	the freshwater prawn Macrobrachium rosenbergii	79:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	3	4	theme	circular	537:544	arg1	CD					557:558	CD	557:558	CD	557:558	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	3	4	theme	circular	537:544	arg1	dichroism					546:554	circular dichroism	537:554	circular dichroism (CD) spectral measurement	537:580	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	1	5	theme	N-linked	275:282	arg1	glycan					284:289	an N-linked glycan	272:289	an N-linked glycan	272:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	6	theme	regioselective	368:381	arg1	formation					393:401	the regioselective disulfide formation	364:401	the regioselective disulfide formation	364:401	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	3	7	theme	spectral	561:568	arg1	measurement					570:580	circular dichroism (CD) spectral measurement	537:580	circular dichroism (CD) spectral measurement	537:580	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	0	8	theme	Macrobrachium	100:112	arg1	rosenbergii					114:124	the freshwater prawn Macrobrachium rosenbergii	79:124	the freshwater prawn Macrobrachium rosenbergii	79:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	1	9	theme	disulfide	383:391	arg1	formation					393:401	the regioselective disulfide formation	364:401	the regioselective disulfide formation	364:401	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	5	10	theme	other	974:978	arg1	peptides					1021:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	4	11	theme	IAG	763:765	arg1	spectra					752:758	CD spectra	749:758	CD spectra of IAG	749:765	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	4	12	attach	attached	709:716	arg2	glycan					702:707	the N-linked glycan	689:707	the N-linked glycan attached at A chain	689:727	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	4	12	attach	attached	709:716	arg3	chain					723:727	A chain	721:727	A chain	721:727	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	5	13	theme	heterodimeric	836:848	arg1	glycopeptide					850:861	insulin-like heterodimeric glycopeptide	823:861	insulin-like heterodimeric glycopeptide having three interchain disulfides	823:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	14	link	N-linked	275:282	arg1	glycan					284:289	an N-linked glycan	272:289	an N-linked glycan	272:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	5	15	theme	glycosylated	980:991	arg1	peptides					1021:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	3	16	from	conformation	649:660	arg1	IAG					665:667	IAG	665:667	IAG	665:667	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	1	17	dep	combination	315:325	arg1	reactions					403:411	reactions	403:411	reactions	403:411	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	5	18	contain	having	863:868	arg1	glycopeptide					850:861	insulin-like heterodimeric glycopeptide	823:861	insulin-like heterodimeric glycopeptide having three interchain disulfides	823:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	5	18	contain	having	863:868	arg2	disulfides					887:896	three interchain disulfides	870:896	three interchain disulfides	870:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	5	19	theme	four-disulfide	993:1006	arg1	peptides					1021:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	20	theme	rosenbergii	198:208	arg1	IAG					176:178	IAG	176:178	IAG	176:178	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	20	theme	rosenbergii	198:208	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	20	theme	rosenbergii	198:208	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	2	21	theme	same	475:478	arg1	manner					480:485	the same manner	471:485	the same manner	471:485	The disulfide isomer of IAG could also be synthesized by the same manner.
31515898	5	22	gly	glycosylated	980:991	arg1	peptides					1021:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	4	23	theme	CD	749:750	arg1	spectra					752:758	CD spectra	749:758	CD spectra of IAG	749:765	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	2	24	theme	disulfide	418:426	arg1	isomer					428:433	The disulfide isomer	414:433	The disulfide isomer of IAG	414:440	The disulfide isomer of IAG could also be synthesized by the same manner.
31515898	0	25	theme	Chemical	0:7	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.	0:125	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	5	26	gly	glycopeptide	850:861	arg2	glycopeptide					850:861	insulin-like heterodimeric glycopeptide	823:861	insulin-like heterodimeric glycopeptide having three interchain disulfides	823:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	3	27	theme	disulfide	601:609	arg1	arrangement					616:626	the disulfide bond arrangement	597:626	the disulfide bond arrangement	597:626	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	0	28	theme	N-glycosylated	22:35	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	5	29	theme	chemical	801:808	arg1	synthesis					810:818	the chemical synthesis	797:818	the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides	797:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	30	theme	heterodimeric	213:225	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	30	theme	heterodimeric	213:225	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	31	theme	Crustacean	127:136	arg1	IAG					176:178	IAG	176:178	IAG	176:178	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	31	theme	Crustacean	127:136	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	31	theme	Crustacean	127:136	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	3	32	theme	conformational	492:505	arg1	analysis					507:514	The conformational analysis	488:514	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement	488:580	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	0	33	theme	androgenic	50:59	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	3	34	theme	peptides	525:532	arg1	analysis					507:514	The conformational analysis	488:514	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement	488:580	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	5	35	theme	glycopeptide	850:861	arg1	synthesis					810:818	the chemical synthesis	797:818	the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides	797:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	4	36	link	N-linked	693:700	arg1	glycan					702:707	the N-linked glycan	689:707	the N-linked glycan attached at A chain	689:727	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	4	37	theme	other	677:681	arg1	hand					683:686	the other hand	673:686	the other hand	673:686	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	0	38	theme	insulin-like	37:48	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	5	39	theme	first	780:784	arg1	report					786:791	the first report	776:791	the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides	776:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	5	39	theme	first	780:784	arg1	This					768:771	This	768:771	This	768:771	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	2	40	theme	IAG	438:440	arg1	isomer					428:433	The disulfide isomer	414:433	The disulfide isomer of IAG	414:440	The disulfide isomer of IAG could also be synthesized by the same manner.
31515898	0	41	theme	factor	67:72	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.	0:125	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	4	42	theme	N-linked	693:700	arg1	glycan					702:707	the N-linked glycan	689:707	the N-linked glycan attached at A chain	689:727	On the other hand, the N-linked glycan attached at A chain showed no effect on CD spectra of IAG.
31515898	3	43	theme	bond	611:614	arg1	arrangement					616:626	the disulfide bond arrangement	597:626	the disulfide bond arrangement	597:626	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	1	44	theme	insulin-like	138:149	arg1	IAG					176:178	IAG	176:178	IAG	176:178	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	44	theme	insulin-like	138:149	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	44	theme	insulin-like	138:149	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	0	45	theme	gland	61:65	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	1	46	theme	solid-phase	330:340	arg1	synthesis					350:358	solid-phase peptide synthesis	330:358	solid-phase peptide synthesis	330:358	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	0	47	gly	N-glycosylated	22:35	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	1	48	theme	androgenic	151:160	arg1	IAG					176:178	IAG	176:178	IAG	176:178	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	48	theme	androgenic	151:160	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	48	theme	androgenic	151:160	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	49	theme	peptide	342:348	arg1	synthesis					350:358	solid-phase peptide synthesis	330:358	solid-phase peptide synthesis	330:358	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	50	contain	having	235:240	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	50	contain	having	235:240	arg2	glycan					284:289	an N-linked glycan	272:289	an N-linked glycan	272:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	50	contain	having	235:240	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	50	contain	having	235:240	arg2	bonds					262:266	four disulfide bonds	247:266	four disulfide bonds	247:266	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	5	51	theme	insulin-like	1008:1019	arg1	peptides					1021:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	other glycosylated four-disulfide insulin-like peptides	974:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	52	theme	gland	162:166	arg1	IAG					176:178	IAG	176:178	IAG	176:178	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	52	theme	gland	162:166	arg1	factor					168:173	Crustacean insulin-like androgenic gland factor	127:173	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii	127:208	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	52	theme	gland	162:166	arg1	peptide					227:233	a heterodimeric peptide	211:233	a heterodimeric peptide having both four disulfide bonds and an N-linked glycan	211:289	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	0	53	from	rosenbergii	114:124	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.	0:125	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	0	53	from	rosenbergii	114:124	arg1	factor					67:72	N-glycosylated insulin-like androgenic gland factor	22:72	N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii	22:124	Chemical synthesis of N-glycosylated insulin-like androgenic gland factor from the freshwater prawn Macrobrachium rosenbergii.
31515898	5	54	theme	peptides	1021:1028	arg1	synthesis					961:969	the synthesis	957:969	the synthesis of other glycosylated four-disulfide insulin-like peptides	957:1028	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	55	theme	disulfide	252:260	arg1	bonds					262:266	four disulfide bonds	247:266	four disulfide bonds	247:266	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	3	56	theme	peptide	641:647	arg1	conformation					649:660	the peptide conformation	637:660	the peptide conformation in IAG	637:667	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	5	57	theme	insulin-like	823:834	arg1	glycopeptide					850:861	insulin-like heterodimeric glycopeptide	823:861	insulin-like heterodimeric glycopeptide having three interchain disulfides	823:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	3	58	theme	dichroism	546:554	arg1	measurement					570:580	circular dichroism (CD) spectral measurement	537:580	circular dichroism (CD) spectral measurement	537:580	The conformational analysis of these peptides by circular dichroism (CD) spectral measurement indicated that the disulfide bond arrangement affected the peptide conformation in IAG.
31515898	5	59	theme	interchain	876:885	arg1	disulfides					887:896	three interchain disulfides	870:896	three interchain disulfides	870:896	This is the first report for the chemical synthesis of insulin-like heterodimeric glycopeptide having three interchain disulfides, and the synthetic strategy shown here might be useful for the synthesis of other glycosylated four-disulfide insulin-like peptides.
31515898	1	60	theme	synthesis	350:358	arg1	formation					393:401	the regioselective disulfide formation	364:401	the regioselective disulfide formation	364:401	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
31515898	1	60	theme	synthesis	350:358	arg1	combination					315:325	the combination	311:325	the combination of solid-phase peptide synthesis	311:358	Crustacean insulin-like androgenic gland factor (IAG) of Macrobrachium rosenbergii, a heterodimeric peptide having both four disulfide bonds and an N-linked glycan, was synthesized by the combination of solid-phase peptide synthesis and the regioselective disulfide formation reactions.
29661325	2	0	theme	alginate	444:451	arg1	solution					402:409	a solution	400:409	a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere	400:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	3	1	theme	PEC	631:633	arg1	hydrogel					645:652	a CS/PGA/SA PEC composite hydrogel	619:652	a CS/PGA/SA PEC composite hydrogel	619:652	CS was gradually dissolved and interacted with PGA and SA to form a CS/PGA/SA PEC composite hydrogel with a homogeneous structure.
29661325	1	2	theme	current	142:148	arg1	study					150:154	the current study	138:154	the current study	138:154	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	9	3	theme	composite	1274:1282	arg1	hydrogels					1284:1292	PEC composite hydrogels	1270:1292	PEC composite hydrogels	1270:1292	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	2	4	theme	acidic	534:539	arg1	atmosphere					541:550	an gaseous acidic atmosphere	523:550	an gaseous acidic atmosphere	523:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	5	5	theme	composition	822:832	arg1	effects					798:804	The effects	794:804	The effects of the hydrogel composition on its structure and properties	794:864	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	2	6	theme	chitosan	352:359	arg1	powder					366:371	A chitosan (CS) powder	350:371	A chitosan (CS) powder	350:371	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	9	7	theme	CS	1348:1349	arg1	hydrogels					1357:1365	the CS based hydrogels	1344:1365	the CS based hydrogels as medical materials	1344:1386	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	6	8	theme	hydrogel	994:1001	arg1	performance					965:975	The drug delivery performance	947:975	The drug delivery performance of the CS/PGA/SA hydrogel	947:1001	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	2	9	theme	gaseous	526:532	arg1	atmosphere					541:550	an gaseous acidic atmosphere	523:550	an gaseous acidic atmosphere	523:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	9	10	theme	SD-A-SGT	1240:1247	arg1	method					1249:1254	the SD-A-SGT method	1236:1254	the SD-A-SGT method for preparing PEC composite hydrogels	1236:1292	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	5	11	theme	XRD	893:895	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	4	12	theme	SD-A-SGT	688:695	arg1	procedure					697:705	The SD-A-SGT procedure	684:705	The SD-A-SGT procedure	684:705	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	4	12	theme	SD-A-SGT	688:695	arg1	able					711:714	able	711:714	able	711:714	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	1	13	theme	polyelectrolyte	269:283	arg1	hydrogels					312:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	9	14	theme	great	1300:1304	arg1	potential					1318:1326	a great application potential	1298:1326	a great application potential	1298:1326	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	3	15	theme	composite	635:643	arg1	hydrogel					645:652	a CS/PGA/SA PEC composite hydrogel	619:652	a CS/PGA/SA PEC composite hydrogel	619:652	CS was gradually dissolved and interacted with PGA and SA to form a CS/PGA/SA PEC composite hydrogel with a homogeneous structure.
29661325	5	16	theme	rheology	898:905	arg1	study					907:911	rheology study	898:911	rheology study	898:911	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	6	17	theme	CS/PGA/SA	984:992	arg1	hydrogel					994:1001	the CS/PGA/SA hydrogel	980:1001	the CS/PGA/SA hydrogel	980:1001	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	9	18	theme	application	1306:1316	arg1	potential					1318:1326	a great application potential	1298:1326	a great application potential	1298:1326	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	9	19	contain	has	1294:1296	arg1	method					1249:1254	the SD-A-SGT method	1236:1254	the SD-A-SGT method for preparing PEC composite hydrogels	1236:1292	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	9	19	contain	has	1294:1296	arg2	potential					1318:1326	a great application potential	1298:1326	a great application potential	1298:1326	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	6	20	theme	model	1043:1047	arg1	piroxicam					1022:1030	piroxicam	1022:1030	piroxicam (PXC)	1022:1036	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	6	20	theme	model	1043:1047	arg1	drug					1049:1052	a model drug	1041:1052	a model drug	1041:1052	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	1	21	theme	novel	159:163	arg1	method					226:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	2	22	theme	glutamic	419:426	arg1	poly					414:417	poly	414:417	poly(glutamic acid) (PGA)	414:438	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	2	22	theme	glutamic	419:426	arg1	acid					428:431	glutamic acid	419:431	glutamic acid	419:431	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	1	23	theme	complexing	285:294	arg1	hydrogels					312:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	4	24	theme	PEC	777:779	arg1	interaction					781:791	the PEC interaction	773:791	the PEC interaction	773:791	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	1	25	theme	semi-dissolution/acidification/sol-gel	165:202	arg1	method					226:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	0	26	theme	chitosan/poly	19:31	arg1	Preparation					0:10	Preparation	0:10	Preparation of the chitosan/poly(glutamic acid)	0:46	Preparation of the chitosan/poly(glutamic acid)/alginate polyelectrolyte complexing hydrogel and study on its drug releasing property.
29661325	5	27	theme	swelling	928:935	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	5	28	theme	SEM	919:921	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	1	29	theme	transition	204:213	arg1	method					226:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	2	30	theme	poly	414:417	arg1	solution					402:409	a solution	400:409	a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere	400:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	1	31	theme	PEC	297:299	arg1	hydrogels					312:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	0	32	from	hydrogel	84:91	arg1	drug					110:113	its drug	106:113	its drug releasing property	106:132	Preparation of the chitosan/poly(glutamic acid)/alginate polyelectrolyte complexing hydrogel and study on its drug releasing property.
29661325	5	33	theme	FTIR	887:890	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	0	34	theme	glutamic	33:40	arg1	chitosan/poly					19:31	the chitosan/poly	15:31	the chitosan/poly(glutamic acid)	15:46	Preparation of the chitosan/poly(glutamic acid)/alginate polyelectrolyte complexing hydrogel and study on its drug releasing property.
29661325	0	34	theme	glutamic	33:40	arg1	acid					42:45	glutamic acid	33:45	glutamic acid	33:45	Preparation of the chitosan/poly(glutamic acid)/alginate polyelectrolyte complexing hydrogel and study on its drug releasing property.
29661325	2	35	theme	slurry	483:488	arg1	mixture					490:496	a semi-dissolved slurry mixture	466:496	a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere	466:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	8	36	theme	responsive	1146:1155	arg1	behaviors					1170:1178	pH responsive drug release behaviors	1143:1178	pH responsive drug release behaviors that were affected by the hydrogel composition	1143:1225	The hydrogel exhibited pH responsive drug release behaviors that were affected by the hydrogel composition.
29661325	4	37	theme	direct	748:753	arg1	method					762:767	direct mixing method	748:767	direct mixing method	748:767	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	6	38	theme	delivery	956:963	arg1	performance					965:975	The drug delivery performance	947:975	The drug delivery performance of the CS/PGA/SA hydrogel	947:1001	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	1	39	theme	SD-A-SGT	216:223	arg1	method					226:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method	157:231	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	1	40	theme	composite	302:310	arg1	hydrogels					312:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	polyelectrolyte complexing (PEC) composite hydrogels	269:320	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	2	41	theme	semi-dissolved	468:481	arg1	mixture					490:496	a semi-dissolved slurry mixture	466:496	a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere	466:550	A chitosan (CS) powder was uniformly dispersed in a solution of poly(glutamic acid) (PGA) and alginate (SA) to form a semi-dissolved slurry mixture that was then exposed to an gaseous acidic atmosphere.
29661325	7	42	from	embedded	1071:1078	arg1	hydrogel					1087:1094	the hydrogel	1083:1094	the hydrogel	1083:1094	PXC was in situ embedded in the hydrogel by the SD-A-SGT method.
29661325	9	43	theme	based	1351:1355	arg1	hydrogels					1357:1365	the CS based hydrogels	1344:1365	the CS based hydrogels as medical materials	1344:1386	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	1	44	theme	hydrogels	312:320	arg1	preparation					254:264	the preparation	250:264	the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only	250:347	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	4	45	theme	method	762:767	arg1	shortcomings					732:743	the shortcomings	728:743	the shortcomings of direct mixing method	728:767	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	8	46	theme	release	1162:1168	arg1	behaviors					1170:1178	pH responsive drug release behaviors	1143:1178	pH responsive drug release behaviors that were affected by the hydrogel composition	1143:1225	The hydrogel exhibited pH responsive drug release behaviors that were affected by the hydrogel composition.
29661325	4	47	theme	mixing	755:760	arg1	method					762:767	direct mixing method	748:767	direct mixing method	748:767	The SD-A-SGT procedure was able to overcome the shortcomings of direct mixing method via the PEC interaction.
29661325	8	48	theme	drug	1157:1160	arg1	behaviors					1170:1178	pH responsive drug release behaviors	1143:1178	pH responsive drug release behaviors that were affected by the hydrogel composition	1143:1225	The hydrogel exhibited pH responsive drug release behaviors that were affected by the hydrogel composition.
29661325	1	49	theme	natural	327:333	arg1	polymers					335:342	natural polymers	327:342	natural polymers only	327:347	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	7	50	from	hydrogel	1087:1094	arg1	embedded					1071:1078	embedded	1071:1078	embedded	1071:1078	PXC was in situ embedded in the hydrogel by the SD-A-SGT method.
29661325	5	51	theme	study	907:911	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	1	52	with	preparation	254:264	arg1	polymers					335:342	natural polymers	327:342	natural polymers only	327:347	In the current study, a novel semi-dissolution/acidification/sol-gel transition (SD-A-SGT) method was explored for the preparation of polyelectrolyte complexing (PEC) composite hydrogels with natural polymers only.
29661325	3	53	theme	homogeneous	661:671	arg1	structure					673:681	a homogeneous structure	659:681	a homogeneous structure	659:681	CS was gradually dissolved and interacted with PGA and SA to form a CS/PGA/SA PEC composite hydrogel with a homogeneous structure.
29661325	0	54	from	study	97:101	arg1	drug					110:113	its drug	106:113	its drug releasing property	106:132	Preparation of the chitosan/poly(glutamic acid)/alginate polyelectrolyte complexing hydrogel and study on its drug releasing property.
29661325	5	55	from	effects	798:804	arg1	structure					841:849	its structure	837:849	its structure	837:849	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	5	55	from	effects	798:804	arg1	properties					855:864	properties	855:864	properties	855:864	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	8	56	theme	hydrogel	1206:1213	arg1	composition					1215:1225	the hydrogel composition	1202:1225	the hydrogel composition	1202:1225	The hydrogel exhibited pH responsive drug release behaviors that were affected by the hydrogel composition.
29661325	9	57	theme	medical	1370:1376	arg1	materials					1378:1386	medical materials	1370:1386	medical materials	1370:1386	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
29661325	5	58	theme	XPS	914:916	arg1	kinetics					937:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics	887:944	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	6	59	theme	drug	951:954	arg1	performance					965:975	The drug delivery performance	947:975	The drug delivery performance of the CS/PGA/SA hydrogel	947:1001	The drug delivery performance of the CS/PGA/SA hydrogel was explored using piroxicam (PXC) as a model drug.
29661325	3	60	theme	CS/PGA/SA	621:629	arg1	hydrogel					645:652	a CS/PGA/SA PEC composite hydrogel	619:652	a CS/PGA/SA PEC composite hydrogel	619:652	CS was gradually dissolved and interacted with PGA and SA to form a CS/PGA/SA PEC composite hydrogel with a homogeneous structure.
29661325	5	61	theme	hydrogel	813:820	arg1	composition					822:832	the hydrogel composition	809:832	the hydrogel composition	809:832	The effects of the hydrogel composition on its structure and properties were investigated by FTIR, XRD, rheology study, XPS, SEM, and swelling kinetics.
29661325	7	62	theme	SD-A-SGT	1103:1110	arg1	method					1112:1117	the SD-A-SGT method	1099:1117	the SD-A-SGT method	1099:1117	PXC was in situ embedded in the hydrogel by the SD-A-SGT method.
29661325	9	63	theme	PEC	1270:1272	arg1	hydrogels					1284:1292	PEC composite hydrogels	1270:1292	PEC composite hydrogels	1270:1292	In all, the SD-A-SGT method for preparing PEC composite hydrogels has a great application potential in constructing the CS based hydrogels as medical materials.
31487514	0	0	theme	molecular	104:112	arg1	weights					114:120	different molecular weights	94:120	different molecular weights	94:120	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	0	1	theme	different	94:102	arg1	weights					114:120	different molecular weights	94:120	different molecular weights	94:120	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	1	2	theme	HNO3-Na2SeO3	330:341	arg1	method					343:348	HNO3-Na2SeO3 method	330:348	HNO3-Na2SeO3 method	330:348	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	19.6 kDa					576:583	19.6 kDa	576:583	19.6 kDa	576:583	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	48.6 kDa					508:515	48.6 kDa	508:515	48.6 kDa	508:515	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	weights					480:486	The average molecular weights	458:486	The average molecular weights (Mw) of WZP1-3	458:501	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	Mw					489:490	Mw	489:490	Mw	489:490	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	37.7 kDa					586:593	37.7 kDa	586:593	37.7 kDa	586:593	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	3	theme	WZP1-3	496:501	arg1	14.5 kDa					596:603	14.5 kDa	596:603	14.5 kDa	596:603	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	4	4	theme	precipitation	751:763	arg1	process					765:771	the ethanol precipitation process	739:771	the ethanol precipitation process	739:771	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	6	5	theme	α-glucosidase-inhibiting	1014:1037	arg1	activity					1068:1075	the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity	1010:1075	the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides	1010:1107	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	4	6	theme	ethanol	743:749	arg1	process					765:771	the ethanol precipitation process	739:771	the ethanol precipitation process	739:771	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	1	7	dep	polysaccharides	164:178	arg1	WZP1					181:184	WZP1	181:184	WZP1	181:184	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	7	dep	polysaccharides	164:178	arg1	WZP3					193:196	WZP3	193:196	WZP3	193:196	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	7	dep	polysaccharides	164:178	arg1	WZP2					187:190	WZP2	187:190	WZP2	187:190	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	0	8	theme	Pleurotus	137:145	arg1	ostreatus					147:155	Pleurotus ostreatus	137:155	Pleurotus ostreatus	137:155	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	5	9	theme	weight	917:922	arg1	complexity					959:968	the molecular weight and the monosaccharide composition complexity	903:968	the molecular weight and the monosaccharide composition complexity	903:968	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	2	10	theme	samples	415:421	arg1	profiles					399:406	The molecular weight distribution profiles	365:406	The molecular weight distribution profiles of all samples except SeWZP2	365:435	The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks.
31487514	4	11	theme	composition	653:663	arg1	proportional					689:700	proportional	689:700	proportional	689:700	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	4	11	theme	composition	653:663	arg1	complexity					624:633	The complexity	620:633	The complexity of monosaccharide composition of WZP1-3	620:673	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	7	12	theme	ostreatus	1197:1205	arg1	polysaccharides					1175:1189	the polysaccharides	1171:1189	the polysaccharides of P. ostreatus	1171:1205	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	7	12	theme	ostreatus	1197:1205	arg1	supplement					1250:1259	a natural health food supplement	1228:1259	a natural health food supplement	1228:1259	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	6	13	dep	P.	1080:1081	arg1	ostreatus					1083:1091	ostreatus	1083:1091	ostreatus	1083:1091	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	2	14	theme	double	444:449	arg1	peaks					451:455	double peaks	444:455	double peaks	444:455	The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks.
31487514	2	15	theme	distribution	386:397	arg1	profiles					399:406	The molecular weight distribution profiles	365:406	The molecular weight distribution profiles of all samples except SeWZP2	365:435	The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks.
31487514	7	16	theme	natural	1230:1236	arg1	polysaccharides					1175:1189	the polysaccharides	1171:1189	the polysaccharides of P. ostreatus	1171:1205	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	7	16	theme	natural	1230:1236	arg1	supplement					1250:1259	a natural health food supplement	1228:1259	a natural health food supplement	1228:1259	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	1	17	dep	simple	305:310	arg1	rapid					313:317	rapid	313:317	rapid	313:317	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	5	18	theme	composition	947:957	arg1	complexity					959:968	the molecular weight and the monosaccharide composition complexity	903:968	the molecular weight and the monosaccharide composition complexity	903:968	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	2	19	theme	weight	379:384	arg1	profiles					399:406	The molecular weight distribution profiles	365:406	The molecular weight distribution profiles of all samples except SeWZP2	365:435	The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks.
31487514	0	20	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	5	21	theme	biological	803:812	arg1	tests					823:827	biological activity tests	803:827	biological activity tests	803:827	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	2	22	theme	molecular	369:377	arg1	profiles					399:406	The molecular weight distribution profiles	365:406	The molecular weight distribution profiles of all samples except SeWZP2	365:435	The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks.
31487514	5	23	theme	activity	814:821	arg1	tests					823:827	biological activity tests	803:827	biological activity tests	803:827	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	1	24	theme	Pleurotus	277:285	arg1	ostreatus					287:295	Pleurotus ostreatus	277:295	Pleurotus ostreatus	277:295	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	0	25	with	polysaccharides	73:87	arg1	weights					114:120	different molecular weights	94:120	different molecular weights	94:120	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	3	26	theme	average	462:468	arg1	19.6 kDa					576:583	19.6 kDa	576:583	19.6 kDa	576:583	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	26	theme	average	462:468	arg1	48.6 kDa					508:515	48.6 kDa	508:515	48.6 kDa	508:515	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	26	theme	average	462:468	arg1	weights					480:486	The average molecular weights	458:486	The average molecular weights (Mw) of WZP1-3	458:501	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	26	theme	average	462:468	arg1	Mw					489:490	Mw	489:490	Mw	489:490	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	26	theme	average	462:468	arg1	37.7 kDa					586:593	37.7 kDa	586:593	37.7 kDa	586:593	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	26	theme	average	462:468	arg1	14.5 kDa					596:603	14.5 kDa	596:603	14.5 kDa	596:603	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	5	27	theme	tests	823:827	arg1	results					792:798	the results	788:798	the results of biological activity tests	788:827	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	5	28	theme	molecular	907:915	arg1	weight					917:922	molecular weight	907:922	molecular weight	907:922	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	1	29	theme	Se-enriched	209:219	arg1	SeWZP2					239:244	SeWZP2	239:244	SeWZP2	239:244	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	29	theme	Se-enriched	209:219	arg1	SeWZP1					231:236	SeWZP1	231:236	SeWZP1	231:236	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	29	theme	Se-enriched	209:219	arg1	products					221:228	their Se-enriched products	203:228	their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3)	203:256	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	29	theme	Se-enriched	209:219	arg1	SeWZP3					250:255	SeWZP3	250:255	SeWZP3	250:255	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	0	30	theme	original	48:55	arg1	polysaccharides					73:87	original and Se-enriched polysaccharides	48:87	original and Se-enriched polysaccharides with different molecular weights	48:120	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	6	31	theme	selenized	975:983	arg1	modification					985:996	The selenized modification	971:996	The selenized modification	971:996	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	7	32	theme	food	1245:1248	arg1	polysaccharides					1175:1189	the polysaccharides	1171:1189	the polysaccharides of P. ostreatus	1171:1205	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	7	32	theme	food	1245:1248	arg1	supplement					1250:1259	a natural health food supplement	1228:1259	a natural health food supplement	1228:1259	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	6	33	theme	polysaccharides	1093:1107	arg1	activity					1068:1075	the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity	1010:1075	the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides	1010:1107	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	3	34	dep	19.6 kDa	576:583	arg1	37.7 kDa					586:593	37.7 kDa	586:593	37.7 kDa	586:593	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	34	dep	19.6 kDa	576:583	arg1	weights					480:486	The average molecular weights	458:486	The average molecular weights (Mw) of WZP1-3	458:501	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	34	dep	19.6 kDa	576:583	arg1	19.6 kDa					576:583	19.6 kDa	576:583	19.6 kDa	576:583	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	34	dep	19.6 kDa	576:583	arg1	48.6 kDa					508:515	48.6 kDa	508:515	48.6 kDa	508:515	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	34	dep	19.6 kDa	576:583	arg1	14.5 kDa					596:603	14.5 kDa	596:603	14.5 kDa	596:603	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	5	35	theme	α-glucosidase	844:856	arg1	activity					869:876	α-glucosidase inhibitory activity	844:876	α-glucosidase inhibitory activity of WZP1-3	844:886	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	5	36	theme	WZP1-3	881:886	arg1	activity					869:876	α-glucosidase inhibitory activity	844:876	α-glucosidase inhibitory activity of WZP1-3	844:886	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	5	37	theme	inhibitory	858:867	arg1	activity					869:876	α-glucosidase inhibitory activity	844:876	α-glucosidase inhibitory activity of WZP1-3	844:886	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	0	38	theme	Se-enriched	61:71	arg1	polysaccharides					73:87	original and Se-enriched polysaccharides	48:87	original and Se-enriched polysaccharides with different molecular weights	48:120	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	1	39	theme	simple	305:310	arg1	method					319:324	a simple, rapid method	303:324	a simple, rapid method	303:324	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	3	40	theme	molecular	470:478	arg1	19.6 kDa					576:583	19.6 kDa	576:583	19.6 kDa	576:583	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	40	theme	molecular	470:478	arg1	48.6 kDa					508:515	48.6 kDa	508:515	48.6 kDa	508:515	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	40	theme	molecular	470:478	arg1	weights					480:486	The average molecular weights	458:486	The average molecular weights (Mw) of WZP1-3	458:501	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	40	theme	molecular	470:478	arg1	Mw					489:490	Mw	489:490	Mw	489:490	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	40	theme	molecular	470:478	arg1	37.7 kDa					586:593	37.7 kDa	586:593	37.7 kDa	586:593	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	40	theme	molecular	470:478	arg1	14.5 kDa					596:603	14.5 kDa	596:603	14.5 kDa	596:603	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	41	theme	SeWZP1-3	562:569	arg1	37.7 kDa					586:593	37.7 kDa	586:593	37.7 kDa	586:593	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	41	theme	SeWZP1-3	562:569	arg1	weights					480:486	The average molecular weights	458:486	The average molecular weights (Mw) of WZP1-3	458:501	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	41	theme	SeWZP1-3	562:569	arg1	19.6 kDa					576:583	19.6 kDa	576:583	19.6 kDa	576:583	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	41	theme	SeWZP1-3	562:569	arg1	14.5 kDa					596:603	14.5 kDa	596:603	14.5 kDa	596:603	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	3	41	theme	SeWZP1-3	562:569	arg1	48.6 kDa					508:515	48.6 kDa	508:515	48.6 kDa	508:515	The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively.
31487514	4	42	theme	monosaccharide	638:651	arg1	composition					653:663	monosaccharide composition	638:663	monosaccharide composition of WZP1-3	638:673	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	7	43	used	utilized	1216:1223	arg2	polysaccharides					1175:1189	the polysaccharides	1171:1189	the polysaccharides of P. ostreatus	1171:1205	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	7	43	used	utilized	1216:1223	arg2	supplement					1250:1259	a natural health food supplement	1228:1259	a natural health food supplement	1228:1259	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	1	44	dep	products	221:228	arg1	SeWZP2					239:244	SeWZP2	239:244	SeWZP2	239:244	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	44	dep	products	221:228	arg1	SeWZP1					231:236	SeWZP1	231:236	SeWZP1	231:236	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	44	dep	products	221:228	arg1	products					221:228	their Se-enriched products	203:228	their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3)	203:256	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	1	44	dep	products	221:228	arg1	SeWZP3					250:255	SeWZP3	250:255	SeWZP3	250:255	Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively.
31487514	4	45	theme	ethanol	709:715	arg1	concentration					717:729	the ethanol concentration	705:729	the ethanol concentration used in the ethanol precipitation process	705:771	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	6	46	theme	P.	1080:1081	arg1	polysaccharides					1093:1107	P. ostreatus polysaccharides	1080:1107	P. ostreatus polysaccharides	1080:1107	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	5	47	theme	monosaccharide	932:945	arg1	composition					947:957	the monosaccharide composition	928:957	the monosaccharide composition	928:957	Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity.
31487514	4	48	theme	WZP1-3	668:673	arg1	composition					653:663	monosaccharide composition	638:663	monosaccharide composition of WZP1-3	638:673	The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process.
31487514	0	49	theme	polysaccharides	73:87	arg1	bioactivities					31:43	bioactivities	31:43	bioactivities	31:43	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	0	49	theme	polysaccharides	73:87	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus.
31487514	6	50	dep	α-glucosidase-inhibiting	1014:1037	arg1	radical-scavenging					1049:1066	radical-scavenging	1049:1066	radical-scavenging	1049:1066	The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides.
31487514	7	51	theme	health	1238:1243	arg1	polysaccharides					1175:1189	the polysaccharides	1171:1189	the polysaccharides of P. ostreatus	1171:1205	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
31487514	7	51	theme	health	1238:1243	arg1	supplement					1250:1259	a natural health food supplement	1228:1259	a natural health food supplement	1228:1259	Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
30031225	2	0	dep	location	565:572	arg1	ii					554:555	ii	554:555	ii	554:555	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	9	1	theme	environmental	1955:1967	arg1	insight					1918:1924	a systematic quantitative insight	1892:1924	a systematic quantitative insight on the impact of nisin	1892:1947	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	1	theme	environmental	1955:1967	arg1	challenge					1969:1977	an environmental challenge	1952:1977	an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities	1952:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	2	theme	model	2054:2058	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	10	3	theme	food	2236:2239	arg1	methods					2252:2258	minimal food processing methods	2228:2258	minimal food processing methods	2228:2258	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	9	4	theme	various	2071:2077	arg1	compositions/complexities					2090:2114	various structural compositions/complexities	2071:2114	various structural compositions/complexities	2071:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	8	5	theme	surface	1820:1826	arg1	growth					1828:1833	surface growth	1820:1833	surface growth	1820:1833	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	1	6	theme	food	277:280	arg1	industry					282:289	the food industry	273:289	the food industry	273:289	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	6	7	theme	biphasic	1362:1369	arg1	system					1371:1376	the developed biphasic system	1348:1376	the developed biphasic system	1348:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	9	8	theme	compositions/complexities	2090:2114	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	4	9	dep	liquid	1028:1033	arg1	systems					1108:1114	the developed viscoelastic systems	1081:1114	the developed viscoelastic systems	1081:1114	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	8	10	theme	monophasic	1675:1684	arg1	systems					1704:1710	monophasic Xanthan gum-based systems	1675:1710	monophasic Xanthan gum-based systems	1675:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	8	11	theme	suboptimal	1840:1849	arg1	10 °C					1864:1868	10 °C	1864:1868	10 °C	1864:1868	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	8	11	theme	suboptimal	1840:1849	arg1	temperature					1851:1861	a suboptimal temperature	1838:1861	a suboptimal temperature (10 °C)	1838:1869	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	9	12	theme	nisin	1943:1947	arg1	impact					1933:1938	the impact	1929:1938	the impact of nisin	1929:1947	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	8	13	theme	gum-based	1694:1702	arg1	systems					1704:1710	monophasic Xanthan gum-based systems	1675:1710	monophasic Xanthan gum-based systems	1675:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	9	14	from	systems	2060:2066	arg1	growth					1986:1991	growth	1986:1991	growth	1986:1991	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	14	from	systems	2060:2066	arg1	organisation					2005:2016	spatial organisation	1997:2016	spatial organisation	1997:2016	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	3	15	theme	food	712:715	arg1	systems					723:729	viscoelastic food model systems	699:729	viscoelastic food model systems of various compositions and internal structure	699:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	0	16	theme	various	124:130	arg1	complexities					143:154	various structural complexities	124:154	various structural complexities	124:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	4	17	theme	innocua	941:947	arg1	dynamics					926:933	The microbial dynamics	912:933	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C	912:973	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	4	18	theme	developed	1085:1093	arg1	systems					1108:1114	the developed viscoelastic systems	1081:1114	the developed viscoelastic systems	1081:1114	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	0	19	theme	complexities	143:154	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	1	20	theme	sensory	416:422	arg1	characteristics					424:438	sensory characteristics	416:438	sensory characteristics	416:438	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	8	21	theme	protective	1718:1727	arg1	role					1729:1732	a protective role	1716:1732	a protective role against the effects of nisin for immersed growth	1716:1781	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	1	22	theme	natural	226:232	arg1	antimicrobials					234:247	natural antimicrobials	226:247	natural antimicrobials	226:247	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	2	23	theme	innocua	671:677	arg1	dynamics					650:657	the microbial dynamics	636:657	the microbial dynamics of Listeria innocua	636:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	3	24	theme	various	734:740	arg1	compositions					742:753	various compositions	734:753	various compositions	734:753	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	4	25	theme	microbial	916:924	arg1	dynamics					926:933	The microbial dynamics	912:933	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C	912:973	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	1	26	theme	products	448:455	arg1	content					404:410	nutritional content	392:410	nutritional content	392:410	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	1	26	theme	products	448:455	arg1	characteristics					424:438	sensory characteristics	416:438	sensory characteristics	416:438	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	3	27	theme	Xanthan	828:834	arg1	gum-based					836:844	monophasic Xanthan gum-based	817:844	monophasic Xanthan gum-based	817:844	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	3	28	theme	structure	768:776	arg1	systems					723:729	viscoelastic food model systems	699:729	viscoelastic food model systems of various compositions and internal structure	699:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	6	29	theme	system	1371:1376	arg1	phase					1339:1343	the protein phase	1327:1343	the protein phase of the developed biphasic system	1327:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	1	30	theme	conventional	334:345	arg1	processing					347:356	conventional processing	334:356	conventional processing	334:356	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	8	31	theme	nisin	1757:1761	arg1	effects					1746:1752	the effects	1742:1752	the effects of nisin	1742:1761	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	3	32	theme	protein-based	876:888	arg1	systems					903:909	protein-based viscoelastic systems	876:909	protein-based viscoelastic systems	876:909	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	4	33	theme	sublethal	1135:1143	arg1	concentration					1145:1157	a sublethal concentration	1133:1157	a sublethal concentration of nisin	1133:1166	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	6	34	theme	developed	1352:1360	arg1	system					1371:1376	the developed biphasic system	1348:1376	the developed biphasic system	1348:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	8	35	theme	immersed	1767:1774	arg1	growth					1776:1781	immersed growth	1767:1781	immersed growth	1767:1781	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	1	36	theme	microbial	180:188	arg1	decontamination					190:204	microbial decontamination	180:204	microbial decontamination	180:204	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	3	37	theme	Xanthan	859:865	arg1	gum/Whey					867:874	biphasic Xanthan gum/Whey	850:874	biphasic Xanthan gum/Whey	850:874	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	2	38	theme	composition	525:535	arg1	impact					500:505	the impact	496:505	the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua	496:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	5	39	theme	organisation	1248:1259	arg1	in/on					1261:1265	spatial organisation in/on	1240:1265	spatial organisation in/on	1240:1265	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	0	40	theme	food	102:105	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	7	41	dep	growth	1572:1577	arg1	surface/immersed					1580:1595	surface/immersed	1580:1595	surface/immersed	1580:1595	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	4	42	dep	in/on	1039:1043	arg1	immersed					1046:1053	immersed	1046:1053	immersed	1046:1053	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	4	42	dep	in/on	1039:1043	arg1	growth					1073:1078	surface colony growth	1058:1078	surface colony growth	1058:1078	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	2	43	theme	natural	604:610	arg1	nisin					626:630	the natural antimicrobial nisin	600:630	(iii) the natural antimicrobial nisin	594:630	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	9	44	theme	quantitative	1905:1916	arg1	insight					1918:1924	a systematic quantitative insight	1892:1924	a systematic quantitative insight on the impact of nisin	1892:1947	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	44	theme	quantitative	1905:1916	arg1	challenge					1969:1977	an environmental challenge	1952:1977	an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities	1952:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	10	45	theme	structural	2179:2188	arg1	composition/complexity					2190:2211	system structural composition/complexity	2172:2211	system structural composition/complexity	2172:2211	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	6	46	theme	protein	1331:1337	arg1	phase					1339:1343	the protein phase	1327:1343	the protein phase of the developed biphasic system	1327:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	0	47	theme	antimicrobial	37:49	arg1	development					62:72	antimicrobial resistance development	37:72	antimicrobial resistance development	37:72	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	5	48	theme	Microscopy	1169:1178	arg1	imaging					1180:1186	Microscopy imaging	1169:1186	Microscopy imaging	1169:1186	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	2	49	theme	microbial	640:648	arg1	dynamics					650:657	the microbial dynamics	636:657	the microbial dynamics of Listeria innocua	636:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	9	50	theme	structural	2079:2088	arg1	compositions/complexities					2090:2114	various structural compositions/complexities	2071:2114	various structural compositions/complexities	2071:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	8	51	theme	inhibitory	1798:1807	arg1	effect					1809:1814	a further inhibitory effect	1788:1814	a further inhibitory effect for surface growth	1788:1833	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	7	52	theme	gum	1619:1621	arg1	concentration					1623:1635	the Xanthan gum concentration	1607:1635	(ii) the Xanthan gum concentration	1602:1635	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	0	53	theme	microbial	14:22	arg1	dynamics					24:31	microbial dynamics	14:31	microbial dynamics	14:31	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	7	54	theme	gum-based	1525:1533	arg1	systems					1535:1541	the monophasic Xanthan gum-based systems	1502:1541	the monophasic Xanthan gum-based systems	1502:1541	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	1	55	dep	such	207:210	arg1	as					212:213	as	212:213	as	212:213	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	7	56	theme	monophasic	1506:1515	arg1	systems					1535:1541	the monophasic Xanthan gum-based systems	1502:1541	the monophasic Xanthan gum-based systems	1502:1541	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	4	57	theme	planktonic	1007:1016	arg1	growth					1018:1023	planktonic growth	1007:1023	planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems	1007:1114	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	8	58	from	viscosity	1662:1670	arg1	systems					1704:1710	monophasic Xanthan gum-based systems	1675:1710	monophasic Xanthan gum-based systems	1675:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	0	59	theme	resistance	51:60	arg1	development					62:72	antimicrobial resistance development	37:72	antimicrobial resistance development	37:72	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	4	60	theme	surface	1058:1064	arg1	growth					1073:1078	surface colony growth	1058:1078	surface colony growth	1058:1078	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	1	61	theme	antimicrobials	234:247	arg1	use					219:221	the use	215:221	the use of natural antimicrobials	215:247	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	9	62	theme	viscoelastic	2036:2047	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	5	63	theme	bacterial	1214:1222	arg1	size					1231:1234	the bacterial colony size	1210:1234	the bacterial colony size	1210:1234	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	7	64	from	distribution	1486:1497	arg1	systems					1535:1541	the monophasic Xanthan gum-based systems	1502:1541	the monophasic Xanthan gum-based systems	1502:1541	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	65	theme	colony	1470:1475	arg1	size					1477:1480	colony size	1470:1480	colony size	1470:1480	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	9	66	from	challenge	1969:1977	arg1	growth					1986:1991	growth	1986:1991	growth	1986:1991	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	66	from	challenge	1969:1977	arg1	organisation					2005:2016	spatial organisation	1997:2016	spatial organisation	1997:2016	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	7	67	dep	size	1477:1480	arg1	the					1466:1468	the	1466:1468	the	1466:1468	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	5	68	used	used	1192:1195	arg2	imaging					1180:1186	Microscopy imaging	1169:1186	Microscopy imaging	1169:1186	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	4	69	from	30 °C	959:963	arg1	dynamics					926:933	The microbial dynamics	912:933	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C	912:973	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	10	70	theme	natural	2265:2271	arg1	antimicrobials					2273:2286	natural antimicrobials	2265:2286	natural antimicrobials	2265:2286	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	7	71	dep	concentration	1623:1635	arg1	ii					1603:1604	ii	1603:1604	ii	1603:1604	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	4	72	from	10 °C	952:956	arg1	dynamics					926:933	The microbial dynamics	912:933	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C	912:973	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	3	73	theme	viscoelastic	699:710	arg1	systems					723:729	viscoelastic food model systems	699:729	viscoelastic food model systems of various compositions and internal structure	699:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	8	74	theme	system	1655:1660	arg1	viscosity					1662:1670	the system viscosity	1651:1670	the system viscosity in monophasic Xanthan gum-based systems	1651:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	7	75	theme	significant	1425:1435	arg1	differences					1437:1447	significant differences	1425:1447	significant differences	1425:1447	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	5	76	dep	size	1231:1234	arg1	systems					1284:1290	the viscoelastic systems	1267:1290	the viscoelastic systems	1267:1290	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	0	77	theme	structural	132:141	arg1	complexities					143:154	various structural complexities	124:154	various structural complexities	124:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	10	78	theme	system	2172:2177	arg1	composition/complexity					2190:2211	system structural composition/complexity	2172:2211	system structural composition/complexity	2172:2211	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	8	79	theme	Xanthan	1686:1692	arg1	systems					1704:1710	monophasic Xanthan gum-based systems	1675:1710	monophasic Xanthan gum-based systems	1675:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	2	80	from	impact	500:505	arg1	dynamics					650:657	the microbial dynamics	636:657	the microbial dynamics of Listeria innocua	636:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	4	81	theme	viscoelastic	1095:1106	arg1	systems					1108:1114	the developed viscoelastic systems	1081:1114	the developed viscoelastic systems	1081:1114	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	3	82	theme	model	717:721	arg1	systems					723:729	viscoelastic food model systems	699:729	viscoelastic food model systems of various compositions and internal structure	699:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	1	83	from	interest	261:268	arg1	industry					282:289	the food industry	273:289	the food industry	273:289	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	4	84	theme	L.	938:939	arg1	innocua					941:947	L. innocua	938:947	L. innocua	938:947	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	6	85	theme	innocua	1316:1322	arg1	growth					1303:1308	Selective growth	1293:1308	Selective growth of L. innocua on the protein phase of the developed biphasic system	1293:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	4	86	theme	nisin	1162:1166	arg1	concentration					1145:1157	a sublethal concentration	1133:1157	a sublethal concentration of nisin	1133:1166	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	3	87	theme	internal	759:766	arg1	structure					768:776	internal structure	759:776	internal structure	759:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	7	88	located	observed	1454:1461	arg1	distribution					1486:1497	distribution	1486:1497	distribution	1486:1497	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	88	located	observed	1454:1461	arg2	differences					1437:1447	significant differences	1425:1447	significant differences	1425:1447	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	88	located	observed	1454:1461	arg1	size					1477:1480	colony size	1470:1480	colony size	1470:1480	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	89	from	size	1477:1480	arg1	systems					1535:1541	the monophasic Xanthan gum-based systems	1502:1541	the monophasic Xanthan gum-based systems	1502:1541	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	90	theme	growth	1572:1577	arg1	concentration					1623:1635	the Xanthan gum concentration	1607:1635	(ii) the Xanthan gum concentration	1602:1635	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	7	90	theme	growth	1572:1577	arg1	type					1564:1567	the type	1560:1567	(i) the type of growth (surface/immersed)	1556:1596	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	2	91	theme	growth	558:563	arg1	location					565:572	(ii) growth location	553:572	(ii) growth location	553:572	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	2	92	theme	Listeria	662:669	arg1	innocua					671:677	Listeria innocua	662:677	Listeria innocua	662:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	3	93	theme	compositions	742:753	arg1	systems					723:729	viscoelastic food model systems	699:729	viscoelastic food model systems of various compositions and internal structure	699:776	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	6	94	theme	Selective	1293:1301	arg1	growth					1303:1308	Selective growth	1293:1308	Selective growth of L. innocua on the protein phase of the developed biphasic system	1293:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	1	95	theme	food	443:446	arg1	products					448:455	food products	443:455	food products	443:455	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	3	96	theme	biphasic	850:857	arg1	gum/Whey					867:874	biphasic Xanthan gum/Whey	850:874	biphasic Xanthan gum/Whey	850:874	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	5	97	theme	viscoelastic	1271:1282	arg1	systems					1284:1290	the viscoelastic systems	1267:1290	the viscoelastic systems	1267:1290	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	0	98	theme	Listeria	77:84	arg1	dynamics					24:31	microbial dynamics	14:31	microbial dynamics	14:31	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	0	98	theme	Listeria	77:84	arg1	development					62:72	antimicrobial resistance development	37:72	antimicrobial resistance development	37:72	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	1	99	theme	Minimal	157:163	arg1	processing					165:174	Minimal processing	157:174	Minimal processing for microbial decontamination, such as the use of natural antimicrobials,	157:248	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	0	100	from	dynamics	24:31	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	0	101	theme	viscoelastic	89:100	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	8	102	contain	had	1712:1714	arg2	effect					1809:1814	a further inhibitory effect	1788:1814	a further inhibitory effect for surface growth	1788:1833	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	8	102	contain	had	1712:1714	arg1	viscosity					1662:1670	the system viscosity	1651:1670	the system viscosity in monophasic Xanthan gum-based systems	1651:1710	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	8	102	contain	had	1712:1714	arg2	role					1729:1732	a protective role	1716:1732	a protective role against the effects of nisin for immersed growth	1716:1781	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	3	103	theme	viscoelastic	890:901	arg1	systems					903:909	protein-based viscoelastic systems	876:909	protein-based viscoelastic systems	876:909	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	5	104	theme	spatial	1240:1246	arg1	in/on					1261:1265	spatial organisation in/on	1240:1265	spatial organisation in/on	1240:1265	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
30031225	3	105	theme	monophasic	817:826	arg1	gum-based					836:844	monophasic Xanthan gum-based	817:844	monophasic Xanthan gum-based	817:844	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	0	106	theme	model	107:111	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	3	107	dep	gum-based	836:844	arg1	systems					903:909	protein-based viscoelastic systems	876:909	protein-based viscoelastic systems	876:909	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	3	107	dep	gum-based	836:844	arg1	i.e.					812:815	i.e.	812:815	i.e.	812:815	More specifically, viscoelastic food model systems of various compositions and internal structure were developed and characterised, i.e. monophasic Xanthan gum-based and biphasic Xanthan gum/Whey protein-based viscoelastic systems.
30031225	2	108	dep	nisin	626:630	arg1	iii					595:597	iii	595:597	iii	595:597	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	2	109	theme	structural	514:523	arg1	composition					525:535	(i) structural composition	510:535	(i) structural composition	510:535	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	0	110	from	development	62:72	arg1	systems					113:119	viscoelastic food model systems	89:119	viscoelastic food model systems of various structural complexities	89:154	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	2	111	theme	antimicrobial	612:624	arg1	nisin					626:630	the natural antimicrobial nisin	600:630	(iii) the natural antimicrobial nisin	594:630	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	10	112	theme	processing	2241:2250	arg1	methods					2252:2258	minimal food processing methods	2228:2258	minimal food processing methods	2228:2258	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	1	113	theme	nutritional	392:402	arg1	content					404:410	nutritional content	392:410	nutritional content	392:410	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	2	114	theme	complexity	541:550	arg1	impact					500:505	the impact	496:505	the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua	496:677	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	10	115	theme	minimal	2228:2234	arg1	methods					2252:2258	minimal food processing methods	2228:2258	minimal food processing methods	2228:2258	This study highlights the importance of accounting for system structural composition/complexity when designing minimal food processing methods with natural antimicrobials.
30031225	8	116	theme	further	1790:1796	arg1	effect					1809:1814	a further inhibitory effect	1788:1814	a further inhibitory effect for surface growth	1788:1833	Furthermore, the system viscosity in monophasic Xanthan gum-based systems had a protective role against the effects of nisin for immersed growth, and a further inhibitory effect for surface growth at a suboptimal temperature (10 °C).
30031225	9	117	from	insight	1918:1924	arg1	impact					1933:1938	the impact	1929:1938	the impact of nisin	1929:1947	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	118	theme	spatial	1997:2003	arg1	organisation					2005:2016	spatial organisation	1997:2016	spatial organisation	1997:2016	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	6	119	theme	first	1399:1403	arg1	time					1405:1408	the first time	1395:1408	the first time	1395:1408	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	4	120	from	growth	1018:1023	arg1	liquid					1028:1033	liquid	1028:1033	liquid	1028:1033	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	4	120	from	growth	1018:1023	arg1	in/on					1039:1043	in/on	1039:1043	in/on	1039:1043	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	2	121	dep	composition	525:535	arg1	i					511:511	i	511:511	i	511:511	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	7	122	theme	Xanthan	1611:1617	arg1	concentration					1623:1635	the Xanthan gum concentration	1607:1635	(ii) the Xanthan gum concentration	1602:1635	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	4	123	from	37 °C	969:973	arg1	dynamics					926:933	The microbial dynamics	912:933	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C	912:973	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	9	124	from	organisation	2005:2016	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	7	125	theme	Xanthan	1517:1523	arg1	systems					1535:1541	the monophasic Xanthan gum-based systems	1502:1541	the monophasic Xanthan gum-based systems	1502:1541	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	9	126	from	growth	1986:1991	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	2	127	theme	study	474:478	arg1	aim					462:464	The aim	458:464	The aim of this study	458:478	The aim of this study was to quantify the impact of (i) structural composition and complexity, (ii) growth location and morphology, and (iii) the natural antimicrobial nisin, on the microbial dynamics of Listeria innocua.
30031225	9	128	theme	innocua	2024:2030	arg1	growth					1986:1991	growth	1986:1991	growth	1986:1991	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	128	theme	innocua	2024:2030	arg1	organisation					2005:2016	spatial organisation	1997:2016	spatial organisation	1997:2016	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	7	129	dep	type	1564:1567	arg1	i					1557:1557	i	1557:1557	i	1557:1557	Additionally, significant differences were observed in the colony size and distribution in the monophasic Xanthan gum-based systems depending on (i) the type of growth (surface/immersed) and (ii) the Xanthan gum concentration.
30031225	6	130	from	growth	1303:1308	arg1	phase					1339:1343	the protein phase	1327:1343	the protein phase of the developed biphasic system	1327:1376	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	0	131	dep	dynamics	24:31	arg1	the					10:12	the	10:12	the	10:12	Modelling the microbial dynamics and antimicrobial resistance development of Listeria in viscoelastic food model systems of various structural complexities.
30031225	6	132	theme	L.	1313:1314	arg1	innocua					1316:1322	L. innocua	1313:1322	L. innocua	1313:1322	Selective growth of L. innocua on the protein phase of the developed biphasic system was observed for the first time.
30031225	9	133	theme	systematic	1894:1903	arg1	insight					1918:1924	a systematic quantitative insight	1892:1924	a systematic quantitative insight on the impact of nisin	1892:1947	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	9	133	theme	systematic	1894:1903	arg1	challenge					1969:1977	an environmental challenge	1952:1977	an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities	1952:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	4	134	theme	colony	1066:1071	arg1	growth					1073:1078	surface colony growth	1058:1078	surface colony growth	1058:1078	The microbial dynamics of L. innocua at 10 °C, 30 °C and 37 °C were monitored and compared for planktonic growth in liquid, or in/on (immersed or surface colony growth) the developed viscoelastic systems, with or without a sublethal concentration of nisin.
30031225	1	135	dep	content	404:410	arg1	the					388:390	the	388:390	the	388:390	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	9	136	theme	food	2049:2052	arg1	systems					2060:2066	viscoelastic food model systems	2036:2066	viscoelastic food model systems of various structural compositions/complexities	2036:2114	These findings give a systematic quantitative insight on the impact of nisin as an environmental challenge on the growth and spatial organisation of L. innocua, in viscoelastic food model systems of various structural compositions/complexities.
30031225	1	137	theme	gaining	253:259	arg1	interest					261:268	gaining interest	253:268	gaining interest in the food industry	253:289	Minimal processing for microbial decontamination, such as the use of natural antimicrobials, is gaining interest in the food industry as these methods are generally milder than conventional processing, therefore better maintaining the nutritional content and sensory characteristics of food products.
30031225	5	138	theme	colony	1224:1229	arg1	size					1231:1234	the bacterial colony size	1210:1234	the bacterial colony size	1210:1234	Microscopy imaging was used to determine the bacterial colony size and spatial organisation in/on the viscoelastic systems.
29444373	10	0	theme	hardening	1696:1704	arg1	development					1640:1650	the development	1636:1650	the development of cold-induced compact morphology and cold hardening	1636:1704	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	9	1	theme	enhanced	1391:1398	arg1	length					1405:1410	the enhanced cell length	1387:1410	the enhanced cell length	1387:1410	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	8	2	from	decrease	1211:1218	arg1	activity					1245:1252	apoplastic peroxidase activity	1223:1252	apoplastic peroxidase activity within the leaf elongating zone of Pincén	1223:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	8	2	from	decrease	1211:1218	arg1	zone					1344:1347	the distal mature zone	1326:1347	the distal mature zone of the leaf	1326:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	10	3	theme	central	1620:1626	arg1	role					1628:1631	a central role	1618:1631	a central role	1618:1631	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	8	4	theme	peroxidase	1234:1243	arg1	activity					1245:1252	apoplastic peroxidase activity	1223:1252	apoplastic peroxidase activity within the leaf elongating zone of Pincén	1223:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	3	5	from	activity	480:487	arg1	cultivars					598:606	two wheat cultivars	588:606	two wheat cultivars with contrasting cold-hardening ability	588:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	9	6	theme	thicker	1416:1422	arg1	walls					1429:1433	thicker cell walls	1416:1433	thicker cell walls	1416:1433	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	10	7	theme	cultivar	1578:1585	arg1	types					1587:1591	cultivar types	1578:1591	cultivar types	1578:1591	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	11	8	theme	hemicellulose	1794:1806	arg1	role					1770:1773	the potential temperature-driven role	1737:1773	the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses	1737:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	1	9	theme	compact	188:194	arg1	plants					196:201	compact plants	188:201	compact plants with small thick leaves after exposure to low temperature	188:259	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	5	10	theme	inter-stomatal	914:927	arg1	cells					939:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	8	11	theme	leaf	1265:1268	arg1	zone					1281:1284	the leaf elongating zone	1261:1284	the leaf elongating zone of Pincén	1261:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	12	theme	peroxidases	1778:1788	arg1	role					1770:1773	the potential temperature-driven role	1737:1773	the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses	1737:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	0	13	theme	wheat	133:137	arg1	plasticity					119:128	cold-induced morpho-anatomical plasticity	88:128	cold-induced morpho-anatomical plasticity of wheat leaves	88:144	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	2	14	theme	cold	298:301	arg1	hardiness					303:311	cold hardiness	298:311	cold hardiness	298:311	These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown.
29444373	8	15	theme	sharp	1205:1209	arg1	decrease					1211:1218	a sharp decrease	1203:1218	a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén	1203:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	16	from	role	1770:1773	arg1	dynamics					1821:1828	cell wall dynamics	1811:1828	cell wall dynamics of grasses	1811:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	4	17	theme	combined	651:658	arg1	microscopy					660:669	A combined microscopy	649:669	A combined microscopy	649:669	A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
29444373	3	18	theme	wheat	592:596	arg1	cultivars					598:606	two wheat cultivars	588:606	two wheat cultivars with contrasting cold-hardening ability	588:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	8	19	theme	Pincén	1289:1294	arg1	zone					1281:1284	the leaf elongating zone	1261:1284	the leaf elongating zone of Pincén	1261:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	20	theme	cell	1811:1814	arg1	dynamics					1821:1828	cell wall dynamics	1811:1828	cell wall dynamics of grasses	1811:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	3	21	theme	cold-hardening	625:638	arg1	ability					640:646	contrasting cold-hardening ability	613:646	contrasting cold-hardening ability	613:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	4	22	dep	winter	744:749	arg1	wheat					794:798	wheat	794:798	wheat	794:798	A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
29444373	6	23	theme	mestome	1017:1023	arg1	cells					1032:1036	mestome sheath cells	1017:1036	mestome sheath cells	1017:1036	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	5	24	contain	had	884:886	arg1	plants					877:882	Cold-grown plants	866:882	Cold-grown plants	866:882	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	5	24	contain	had	884:886	arg2	cells					939:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	8	25	theme	three-fold	1303:1312	arg1	increase					1314:1321	a three-fold increase	1301:1321	a three-fold increase in the distal mature zone of the leaf	1301:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	26	theme	grasses	1833:1839	arg1	dynamics					1821:1828	cell wall dynamics	1811:1828	cell wall dynamics of grasses	1811:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	0	27	dep	wheat	133:137	arg1	leaves					139:144	leaves	139:144	leaves	139:144	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	0	28	from	Changes	0:6	arg1	composition					56:66	cell wall composition	46:66	cell wall composition	46:66	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	0	28	from	Changes	0:6	arg1	activity					33:40	apoplastic peroxidase activity	11:40	apoplastic peroxidase activity	11:40	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	8	29	theme	distal	1330:1335	arg1	zone					1344:1347	the distal mature zone	1326:1347	the distal mature zone of the leaf	1326:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	30	theme	potential	1741:1749	arg1	role					1770:1773	the potential temperature-driven role	1737:1773	the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses	1737:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	0	31	theme	cold-induced	88:99	arg1	plasticity					119:128	cold-induced morpho-anatomical plasticity	88:128	cold-induced morpho-anatomical plasticity of wheat leaves	88:144	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	6	32	theme	thicker	978:984	arg1	walls					986:990	thicker walls	978:990	thicker walls	978:990	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	6	33	from	walls	986:990	arg1	cells					1032:1036	mestome sheath cells	1017:1036	mestome sheath cells	1017:1036	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	6	33	from	walls	986:990	arg1	vessels					1005:1011	metaxylem vessels	995:1011	metaxylem vessels	995:1011	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	6	34	theme	wall	1071:1074	arg1	components					1076:1085	wall components	1071:1085	wall components	1071:1085	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	1	35	theme	Temperate	147:155	arg1	wheat					174:178	wheat	174:178	wheat	174:178	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	1	35	theme	Temperate	147:155	arg1	grasses					157:163	Temperate grasses	147:163	Temperate grasses	147:163	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	3	36	theme	leaf	420:423	arg1	structure					443:451	leaf morpho-anatomical structure	420:451	leaf morpho-anatomical structure	420:451	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	10	37	theme	different	1465:1473	arg1	response					1475:1482	The different response	1461:1482	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types	1461:1591	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	10	38	theme	cold-induced	1655:1666	arg1	morphology					1676:1685	cold-induced compact morphology	1655:1685	cold-induced compact morphology	1655:1685	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	0	39	theme	apoplastic	11:20	arg1	activity					33:40	apoplastic peroxidase activity	11:40	apoplastic peroxidase activity	11:40	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	5	40	theme	warm-grown	950:959	arg1	plants					961:966	warm-grown plants	950:966	warm-grown plants	950:966	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	3	41	theme	temperature	405:415	arg1	effects					390:396	the effects	386:396	the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability	386:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	42	theme	cell	454:457	arg1	composition					464:474	cell wall composition	454:474	cell wall composition	454:474	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	7	43	theme	winter	1159:1164	arg1	cultivar					1166:1173	the winter cultivar	1155:1173	the winter cultivar	1155:1173	These effects were more pronounced in the winter cultivar (Pincén).
29444373	9	44	from	cultivar	1443:1450	arg1	consistent					1371:1380	consistent	1371:1380	consistent	1371:1380	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	3	45	theme	extracellular	492:504	arg1	peroxidases					506:516	extracellular peroxidases	492:516	extracellular peroxidases	492:516	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	0	46	theme	cell	46:49	arg1	composition					56:66	cell wall composition	46:66	cell wall composition	46:66	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	10	47	theme	morphology	1676:1685	arg1	development					1640:1650	the development	1636:1650	the development of cold-induced compact morphology and cold hardening	1636:1704	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	10	48	theme	activity	1528:1535	arg1	hemicellulose					1541:1553	hemicellulose	1541:1553	hemicellulose between leaf zones and cultivar types	1541:1591	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	10	48	theme	activity	1528:1535	arg1	temperature					1491:1501	low temperature	1487:1501	low temperature of apoplastic peroxidase activity	1487:1535	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	6	49	theme	hemicellulose	1102:1114	arg1	walls					986:990	thicker walls	978:990	thicker walls	978:990	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	10	50	theme	apoplastic	1506:1515	arg1	activity					1528:1535	apoplastic peroxidase activity	1506:1535	apoplastic peroxidase activity	1506:1535	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	1	51	theme	low	245:247	arg1	temperature					249:259	low temperature	245:259	low temperature	245:259	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	4	52	theme	5 °C	847:850	arg1	temperature					853:863	constant warm (25 °C) or cool (5 °C) temperature	816:863	temperature	853:863	A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
29444373	10	53	theme	leaf	1563:1566	arg1	zones					1568:1572	leaf zones	1563:1572	leaf zones	1563:1572	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	3	54	theme	cell	543:546	arg1	elongation					548:557	cell elongation	543:557	cell elongation	543:557	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	9	55	theme	cell	1400:1403	arg1	length					1405:1410	the enhanced cell length	1387:1410	the enhanced cell length	1387:1410	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	10	56	theme	cold	1691:1694	arg1	hardening					1696:1704	cold hardening	1691:1704	cold hardening	1691:1704	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	8	57	theme	apoplastic	1223:1232	arg1	activity					1245:1252	apoplastic peroxidase activity	1223:1252	apoplastic peroxidase activity within the leaf elongating zone of Pincén	1223:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	3	58	theme	wall	568:571	arg1	thickening					573:582	cell wall thickening	563:582	cell wall thickening	563:582	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	9	59	theme	cell	1424:1427	arg1	walls					1429:1433	thicker cell walls	1416:1433	thicker cell walls	1416:1433	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	4	60	theme	cool	841:844	arg1	temperature					853:863	constant warm (25 °C) or cool (5 °C) temperature	816:863	temperature	853:863	A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
29444373	9	61	from	5 °C	1455:1458	arg1	consistent					1371:1380	consistent	1371:1380	consistent	1371:1380	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	4	62	theme	biochemical	675:685	arg1	approach					687:694	biochemical approach	675:694	biochemical approach	675:694	A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
29444373	5	63	theme	shorter	888:894	arg1	cells					939:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	8	64	theme	elongating	1270:1279	arg1	zone					1281:1284	the leaf elongating zone	1261:1284	the leaf elongating zone of Pincén	1261:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	11	65	theme	wall	1816:1819	arg1	dynamics					1821:1828	cell wall dynamics	1811:1828	cell wall dynamics of grasses	1811:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	3	66	theme	contrasting	613:623	arg1	ability					640:646	contrasting cold-hardening ability	613:646	contrasting cold-hardening ability	613:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	5	67	theme	longer	907:912	arg1	cells					939:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	6	68	theme	sheath	1025:1030	arg1	cells					1032:1036	mestome sheath cells	1017:1036	mestome sheath cells	1017:1036	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	2	69	theme	underlying	324:333	arg1	mechanisms					335:344	their underlying mechanisms	318:344	their underlying mechanisms	318:344	These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown.
29444373	3	70	with	cultivars	598:606	arg1	ability					640:646	contrasting cold-hardening ability	613:646	contrasting cold-hardening ability	613:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	5	71	theme	Cold-grown	866:875	arg1	plants					877:882	Cold-grown plants	866:882	Cold-grown plants	866:882	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	7	72	from	pronounced	1141:1150	arg1	cultivar					1166:1173	the winter cultivar	1155:1173	the winter cultivar	1155:1173	These effects were more pronounced in the winter cultivar (Pincén).
29444373	6	73	theme	metaxylem	995:1003	arg1	vessels					1005:1011	metaxylem vessels	995:1011	metaxylem vessels	995:1011	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	0	74	theme	morpho-anatomical	101:117	arg1	plasticity					119:128	cold-induced morpho-anatomical plasticity	88:128	cold-induced morpho-anatomical plasticity of wheat leaves	88:144	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	3	75	theme	cell	563:566	arg1	thickening					573:582	cell wall thickening	563:582	cell wall thickening	563:582	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	7	76	from	cultivar	1166:1173	arg1	pronounced					1141:1150	pronounced	1141:1150	pronounced	1141:1150	These effects were more pronounced in the winter cultivar (Pincén).
29444373	11	77	theme	temperature-driven	1751:1768	arg1	role					1770:1773	the potential temperature-driven role	1737:1773	the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses	1737:1839	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	6	78	theme	components	1076:1085	arg1	accumulation					1055:1066	accumulation	1055:1066	accumulation of wall components	1055:1085	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	8	79	theme	mature	1337:1342	arg1	zone					1344:1347	the distal mature zone	1326:1347	the distal mature zone of the leaf	1326:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	3	80	from	effects	390:396	arg1	structure					443:451	leaf morpho-anatomical structure	420:451	leaf morpho-anatomical structure	420:451	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	80	from	effects	390:396	arg1	activity					480:487	activity	480:487	activity	480:487	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	80	from	effects	390:396	arg1	composition					464:474	cell wall composition	454:474	cell wall composition	454:474	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	8	81	from	increase	1314:1321	arg1	activity					1245:1252	apoplastic peroxidase activity	1223:1252	apoplastic peroxidase activity within the leaf elongating zone of Pincén	1223:1294	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	8	81	from	increase	1314:1321	arg1	zone					1344:1347	the distal mature zone	1326:1347	the distal mature zone of the leaf	1326:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	5	82	theme	epidermal	929:937	arg1	cells					939:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	shorter leaves but longer inter-stomatal epidermal cells	888:943	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	5	83	dep	shorter	888:894	arg1	leaves					896:901	leaves	896:901	leaves	896:901	Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants.
29444373	3	84	theme	morpho-anatomical	425:441	arg1	structure					443:451	leaf morpho-anatomical structure	420:451	leaf morpho-anatomical structure	420:451	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	0	85	theme	peroxidase	22:31	arg1	activity					33:40	apoplastic peroxidase activity	11:40	apoplastic peroxidase activity	11:40	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	8	86	theme	leaf	1356:1359	arg1	zone					1344:1347	the distal mature zone	1326:1347	the distal mature zone of the leaf	1326:1359	Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf.
29444373	1	87	with	plants	196:201	arg1	thick					214:218	thick	214:218	thick	214:218	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	3	88	theme	wall	459:462	arg1	composition					464:474	cell wall composition	454:474	cell wall composition	454:474	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	0	89	theme	wall	51:54	arg1	composition					56:66	cell wall composition	46:66	cell wall composition	46:66	Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves.
29444373	9	90	from	consistent	1371:1380	arg1	cultivar					1443:1450	this cultivar	1438:1450	this cultivar	1438:1450	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	3	91	theme	low	401:403	arg1	temperature					405:415	low temperature	401:415	low temperature	401:415	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	10	92	theme	low	1487:1489	arg1	temperature					1491:1501	low temperature	1487:1501	low temperature of apoplastic peroxidase activity	1487:1535	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	11	93	theme	New	1707:1709	arg1	insights					1711:1718	New insights	1707:1718	New insights	1707:1718	New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
29444373	6	94	contain	had	974:976	arg1	They					969:972	They	969:972	They	969:972	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	6	94	contain	had	974:976	arg2	walls					986:990	thicker walls	978:990	thicker walls	978:990	They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose.
29444373	3	95	from	composition	464:474	arg1	cultivars					598:606	two wheat cultivars	588:606	two wheat cultivars with contrasting cold-hardening ability	588:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	96	theme	peroxidases	506:516	arg1	structure					443:451	leaf morpho-anatomical structure	420:451	leaf morpho-anatomical structure	420:451	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	96	theme	peroxidases	506:516	arg1	activity					480:487	activity	480:487	activity	480:487	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	96	theme	peroxidases	506:516	arg1	composition					464:474	cell wall composition	454:474	cell wall composition	454:474	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	1	97	dep	thick	214:218	arg1	leaves					220:225	leaves	220:225	leaves after exposure to low temperature	220:259	Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature.
29444373	9	98	with	consistent	1371:1380	arg1	walls					1429:1433	thicker cell walls	1416:1433	thicker cell walls	1416:1433	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	9	98	with	consistent	1371:1380	arg1	length					1405:1410	the enhanced cell length	1387:1410	the enhanced cell length	1387:1410	This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
29444373	10	99	theme	compact	1668:1674	arg1	morphology					1676:1685	cold-induced compact morphology	1655:1685	cold-induced compact morphology	1655:1685	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	10	100	theme	peroxidase	1517:1526	arg1	activity					1528:1535	apoplastic peroxidase activity	1506:1535	apoplastic peroxidase activity	1506:1535	The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening.
29444373	3	101	from	structure	443:451	arg1	cultivars					598:606	two wheat cultivars	588:606	two wheat cultivars with contrasting cold-hardening ability	588:646	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	3	102	theme	key	530:532	arg1	roles					534:538	key roles	530:538	key roles	530:538	Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability.
29444373	7	103	dep	pronounced	1141:1150	arg1	Pincén					1176:1181	Pincén	1176:1181	Pincén	1176:1181	These effects were more pronounced in the winter cultivar (Pincén).
31274284	2	0	theme	scanning	450:457	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy	450:477	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	2	1	dep	transform	417:425	arg1	infrared					427:434	infrared	427:434	transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry	417:534	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	3	2	dep	properties	552:561	arg1	properties					552:561	The mechanical properties	537:561	The mechanical properties (toughness, burst strength, and distance to burst)	537:612	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	2	dep	properties	552:561	arg1	toughness					564:572	toughness	564:572	toughness	564:572	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	2	dep	properties	552:561	arg1	strength					581:588	burst strength	575:588	burst strength	575:588	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	2	dep	properties	552:561	arg1	distance					595:602	distance	595:602	distance to burst	595:611	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	1	3	theme	improved	281:288	arg1	properties					313:322	improved optical and mechanical properties	281:322	improved optical and mechanical properties	281:322	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	2	4	dep	Fourier	409:415	arg1	transform					417:425	transform	417:425	transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry	417:534	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	5	5	theme	bacterial	851:859	arg1	matrix					871:876	the bacterial cellulose matrix	847:876	the bacterial cellulose matrix	847:876	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	7	6	theme	2105.52	1095:1101	arg1	g					1103:1103	2105.52 g	1095:1103	2105.52 g	1095:1103	The burst strength values obtained ranged between 43.74 and 2105.52 g.
31274284	0	7	theme	vinyl	89:93	arg1	Poly					84:87	Poly	84:87	Poly(vinyl alcohol)	84:102	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	7	theme	vinyl	89:93	arg1	alcohol					95:101	vinyl alcohol	89:101	vinyl alcohol	89:101	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	1	8	theme	optical	290:296	arg1	properties					313:322	improved optical and mechanical properties	281:322	improved optical and mechanical properties	281:322	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	10	9	theme	UV	1312:1313	arg1	region					1315:1320	the UV region	1308:1320	the UV region	1308:1320	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	5	10	theme	plasticizing	914:925	arg1	behavior					927:934	a noticeable plasticizing behavior	901:934	a noticeable plasticizing behavior	901:934	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	5	11	theme	noticeable	903:912	arg1	behavior					927:934	a noticeable plasticizing behavior	901:934	a noticeable plasticizing behavior	901:934	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	1	12	theme	bacterial	221:229	arg1	cellulose					231:239	bacterial cellulose	221:239	bacterial cellulose	221:239	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	11	13	theme	UV	1485:1486	arg1	B					1500:1500	B	1500:1500	B	1500:1500	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	13	theme	UV	1485:1486	arg1	C					1507:1507	C	1507:1507	C	1507:1507	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	13	theme	UV	1485:1486	arg1	A					1497:1497	A	1497:1497	A	1497:1497	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	13	theme	UV	1485:1486	arg1	regions					1488:1494	the UV regions	1481:1494	the UV regions (A, B, and C)	1481:1508	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	3	14	theme	composite	681:689	arg1	films					691:695	the composite films	677:695	the composite films	677:695	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	5	15	from	effect	837:842	arg1	matrix					871:876	the bacterial cellulose matrix	847:876	the bacterial cellulose matrix	847:876	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	5	16	theme	vinyl	801:805	arg1	Poly					796:799	Poly	796:799	Poly(vinyl alcohol)	796:814	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	5	16	theme	vinyl	801:805	arg1	alcohol					807:813	vinyl alcohol	801:813	vinyl alcohol	801:813	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	1	17	theme	mechanical	302:311	arg1	properties					313:322	improved optical and mechanical properties	281:322	improved optical and mechanical properties	281:322	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	10	18	theme	vinyl	1384:1388	arg1	poly					1379:1382	poly	1379:1382	poly(vinyl alcohol)	1379:1397	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	10	18	theme	vinyl	1384:1388	arg1	alcohol					1390:1396	vinyl alcohol	1384:1396	vinyl alcohol	1384:1396	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	10	19	theme	films	1366:1370	arg1	properties					1348:1357	the UV-barrier properties	1333:1357	the UV-barrier properties of the films	1333:1370	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	11	20	dep	regions	1488:1494	arg1	B					1500:1500	B	1500:1500	B	1500:1500	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	20	dep	regions	1488:1494	arg1	C					1507:1507	C	1507:1507	C	1507:1507	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	20	dep	regions	1488:1494	arg1	A					1497:1497	A	1497:1497	A	1497:1497	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	20	dep	regions	1488:1494	arg1	regions					1488:1494	the UV regions	1481:1494	the UV regions (A, B, and C)	1481:1508	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	6	21	theme	bacterial	941:949	arg1	composites					967:976	The bacterial cellulose-based composites	937:976	The bacterial cellulose-based composites	937:976	The bacterial cellulose-based composites showed toughness values ranging from 0.22 to 2.60 MJ/m3.
31274284	6	22	dep	2.60	1023:1026	arg1	to					1020:1021	to	1020:1021	to	1020:1021	The bacterial cellulose-based composites showed toughness values ranging from 0.22 to 2.60 MJ/m3.
31274284	11	23	dep	48.51	1527:1531	arg1	to					1524:1525	to	1524:1525	to	1524:1525	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	9	24	theme	film	1161:1164	arg1	solubility					1166:1175	The film solubility	1157:1175	The film solubility on water	1157:1184	The film solubility on water ranged from 9.37 to 31.65%, and the water retention ranged from 78.26 to 364.78%.
31274284	3	25	theme	films	691:695	arg1	strength					581:588	burst strength	575:588	burst strength	575:588	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	solubility					615:624	solubility	615:624	solubility	615:624	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	properties					552:561	The mechanical properties	537:561	The mechanical properties (toughness, burst strength, and distance to burst)	537:612	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	properties					663:672	light barrier properties	649:672	light barrier properties	649:672	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	distance					595:602	distance	595:602	distance to burst	595:611	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	toughness					564:572	toughness	564:572	toughness	564:572	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	25	theme	films	691:695	arg1	adsorption					633:642	water adsorption	627:642	water adsorption	627:642	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	26	theme	light	649:653	arg1	properties					663:672	light barrier properties	649:672	light barrier properties	649:672	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	0	27	theme	Composite	0:8	arg1	Films					10:14	Composite Films	0:14	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.	0:157	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	10	28	theme	opacity	1429:1435	arg1	values					1437:1442	the transparency and opacity values	1408:1442	the transparency and opacity values of the samples	1408:1457	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	5	29	theme	reinforcing	825:835	arg1	effect					837:842	a reinforcing effect	823:842	a reinforcing effect on the bacterial cellulose matrix	823:876	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	0	30	theme	UV-Barrier	21:30	arg1	Properties					32:41	UV-Barrier Properties	21:41	UV-Barrier Properties of Bacterial Cellulose	21:64	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	31	theme	Puncture	105:112	arg1	Properties					114:123	Puncture Properties	105:123	Puncture Properties	105:123	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	9	32	dep	31.65	1206:1210	arg1	to					1203:1204	to	1203:1204	to	1203:1204	The film solubility on water ranged from 9.37 to 31.65%, and the water retention ranged from 78.26 to 364.78%.
31274284	4	33	theme	Polynomial	713:722	arg1	models					724:729	Polynomial models	713:729	Polynomial models obtained	713:738	Polynomial models obtained allowed us to predict the behavior of these properties.
31274284	7	34	theme	strength	1045:1052	arg1	values					1054:1059	The burst strength values	1035:1059	The burst strength values obtained	1035:1068	The burst strength values obtained ranged between 43.74 and 2105.52 g.
31274284	1	35	theme	good	328:331	arg1	property					344:351	good UV-barrier property	328:351	good UV-barrier property	328:351	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	3	36	theme	mechanical	541:550	arg1	distance					595:602	distance	595:602	distance to burst	595:611	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	36	theme	mechanical	541:550	arg1	toughness					564:572	toughness	564:572	toughness	564:572	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	36	theme	mechanical	541:550	arg1	strength					581:588	burst strength	575:588	burst strength	575:588	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	36	theme	mechanical	541:550	arg1	properties					552:561	The mechanical properties	537:561	The mechanical properties (toughness, burst strength, and distance to burst)	537:612	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	10	37	theme	UV-barrier	1337:1346	arg1	properties					1348:1357	the UV-barrier properties	1333:1357	the UV-barrier properties of the films	1333:1370	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	2	38	theme	scanning	515:522	arg1	calorimetry					524:534	differential scanning calorimetry	502:534	differential scanning calorimetry	502:534	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	1	39	theme	UV-barrier	333:342	arg1	property					344:351	good UV-barrier property	328:351	good UV-barrier property	328:351	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	3	40	theme	burst	575:579	arg1	strength					581:588	burst strength	575:588	burst strength	575:588	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	3	40	theme	burst	575:579	arg1	properties					552:561	The mechanical properties	537:561	The mechanical properties (toughness, burst strength, and distance to burst)	537:612	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	1	41	theme	study	175:179	arg1	aim					163:165	The aim	159:165	The aim of this study	159:179	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	2	42	theme	differential	502:513	arg1	calorimetry					524:534	differential scanning calorimetry	502:534	differential scanning calorimetry	502:534	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	10	43	from	transmittance	1291:1303	arg1	region					1315:1320	the UV region	1308:1320	the UV region	1308:1320	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	0	44	theme	Cellulose	56:64	arg1	Properties					32:41	UV-Barrier Properties	21:41	UV-Barrier Properties of Bacterial Cellulose	21:64	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	10	45	theme	samples	1451:1457	arg1	values					1437:1442	the transparency and opacity values	1408:1442	the transparency and opacity values of the samples	1408:1457	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	8	46	dep	4.94	1148:1151	arg1	to					1145:1146	to	1145:1146	to	1145:1146	The distance to burst ranged from 0.39 to 4.94 mm.
31274284	5	47	theme	cellulose	861:869	arg1	matrix					871:876	the bacterial cellulose matrix	847:876	the bacterial cellulose matrix	847:876	Poly(vinyl alcohol) showed a reinforcing effect on the bacterial cellulose matrix, while glycerol showed a noticeable plasticizing behavior.
31274284	0	48	theme	Bacterial	46:54	arg1	Cellulose					56:64	Bacterial Cellulose	46:64	Bacterial Cellulose	46:64	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	1	49	theme	vinyl	261:265	arg1	poly					256:259	poly	256:259	poly(vinyl alcohol)	256:274	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	1	49	theme	vinyl	261:265	arg1	alcohol					267:273	vinyl alcohol	261:273	vinyl alcohol	261:273	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	7	50	theme	burst	1039:1043	arg1	values					1054:1059	The burst strength values	1035:1059	The burst strength values obtained	1035:1068	The burst strength values obtained ranged between 43.74 and 2105.52 g.
31274284	11	51	theme	vinyl	1560:1564	arg1	concentration					1575:1587	the poly(vinyl alcohol) concentration	1551:1587	the poly(vinyl alcohol) concentration	1551:1587	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	4	52	theme	properties	784:793	arg1	behavior					766:773	the behavior	762:773	the behavior of these properties	762:793	Polynomial models obtained allowed us to predict the behavior of these properties.
31274284	0	53	with	Films	10:14	arg1	Poly					84:87	Poly	84:87	Poly(vinyl alcohol)	84:102	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	53	with	Films	10:14	arg1	alcohol					95:101	vinyl alcohol	89:101	vinyl alcohol	89:101	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	53	with	Films	10:14	arg1	Glycerol					71:78	Glycerol	71:78	Glycerol	71:78	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	53	with	Films	10:14	arg1	Properties					32:41	UV-Barrier Properties	21:41	UV-Barrier Properties of Bacterial Cellulose	21:64	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	11	54	from	transmittance	1464:1476	arg1	B					1500:1500	B	1500:1500	B	1500:1500	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	54	from	transmittance	1464:1476	arg1	C					1507:1507	C	1507:1507	C	1507:1507	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	54	from	transmittance	1464:1476	arg1	A					1497:1497	A	1497:1497	A	1497:1497	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	11	54	from	transmittance	1464:1476	arg1	regions					1488:1494	the UV regions	1481:1494	the UV regions (A, B, and C)	1481:1508	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	9	55	dep	364.78	1259:1264	arg1	to					1256:1257	to	1256:1257	to	1256:1257	The film solubility on water ranged from 9.37 to 31.65%, and the water retention ranged from 78.26 to 364.78%.
31274284	0	56	theme	Swelling	142:149	arg1	Degree					151:156	Swelling Degree	142:156	Swelling Degree	142:156	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	11	57	theme	alcohol	1566:1572	arg1	concentration					1575:1587	the poly(vinyl alcohol) concentration	1551:1587	the poly(vinyl alcohol) concentration	1551:1587	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	9	58	from	solubility	1166:1175	arg1	water					1180:1184	water	1180:1184	water	1180:1184	The film solubility on water ranged from 9.37 to 31.65%, and the water retention ranged from 78.26 to 364.78%.
31274284	6	59	theme	cellulose-based	951:965	arg1	composites					967:976	The bacterial cellulose-based composites	937:976	The bacterial cellulose-based composites	937:976	The bacterial cellulose-based composites showed toughness values ranging from 0.22 to 2.60 MJ/m3.
31274284	10	60	theme	transparency	1412:1423	arg1	values					1437:1442	the transparency and opacity values	1408:1442	the transparency and opacity values of the samples	1408:1457	Glycerol decreased the transmittance in the UV region, improving the UV-barrier properties of the films, while poly(vinyl alcohol) improved the transparency and opacity values of the samples.
31274284	11	61	theme	poly	1555:1558	arg1	concentration					1575:1587	the poly(vinyl alcohol) concentration	1551:1587	the poly(vinyl alcohol) concentration	1551:1587	The transmittance in the UV regions (A, B, and C) ranged from 1 to 48.51%, increasing with the poly(vinyl alcohol) concentration.
31274284	3	62	theme	water	627:631	arg1	adsorption					633:642	water adsorption	627:642	water adsorption	627:642	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	0	63	dep	Films	10:14	arg1	Properties					114:123	Puncture Properties	105:123	Puncture Properties	105:123	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	63	dep	Films	10:14	arg1	Degree					151:156	Swelling Degree	142:156	Swelling Degree	142:156	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	0	63	dep	Films	10:14	arg1	Solubility					126:135	Solubility	126:135	Solubility	126:135	Composite Films with UV-Barrier Properties of Bacterial Cellulose with Glycerol and Poly(vinyl alcohol): Puncture Properties, Solubility, and Swelling Degree.
31274284	1	64	with	films	206:210	arg1	property					344:351	good UV-barrier property	328:351	good UV-barrier property	328:351	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	1	64	with	films	206:210	arg1	properties					313:322	improved optical and mechanical properties	281:322	improved optical and mechanical properties	281:322	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	9	65	theme	water	1222:1226	arg1	retention					1228:1236	the water retention	1218:1236	the water retention	1218:1236	The film solubility on water ranged from 9.37 to 31.65%, and the water retention ranged from 78.26 to 364.78%.
31274284	1	66	theme	composite	196:204	arg1	films					206:210	composite films	196:210	composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property	196:351	The aim of this study was to develop composite films based on bacterial cellulose, glycerol, and poly(vinyl alcohol) with improved optical and mechanical properties and good UV-barrier property.
31274284	2	67	theme	electron	459:466	arg1	microscopy					468:477	scanning electron microscopy	450:477	scanning electron microscopy	450:477	The interaction among the compounds was analyzed using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetry, and differential scanning calorimetry.
31274284	3	68	theme	barrier	655:661	arg1	properties					663:672	light barrier properties	649:672	light barrier properties	649:672	The mechanical properties (toughness, burst strength, and distance to burst), solubility, water adsorption, and light barrier properties of the composite films were evaluated.
31274284	6	69	theme	toughness	985:993	arg1	values					995:1000	toughness values	985:1000	toughness values ranging from 0.22 to 2.60 MJ/m3	985:1032	The bacterial cellulose-based composites showed toughness values ranging from 0.22 to 2.60 MJ/m3.
31669273	3	0	theme	cell	677:680	arg1	apoptosis					682:690	cell apoptosis	677:690	cell apoptosis	677:690	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	6	1	theme	spleen	1027:1032	arg1	lymphocytes					1034:1044	spleen lymphocytes	1027:1044	spleen lymphocytes	1027:1044	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	2	2	theme	in	352:353	arg1	activity					371:378	in vitro and in vivo anti-tumor activity	339:378	in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	339:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	7	3	theme	peripheral	1196:1205	arg1	blood					1207:1211	peripheral blood	1196:1211	peripheral blood	1196:1211	Furthermore, APS was capable of up-regulating the expression of IL-2, TNF-α and IFN-γ in peripheral blood.
31669273	5	4	dep	in	810:811	arg1	vivo					813:816	vivo	813:816	vivo	813:816	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	1	5	theme	anti-cancer	175:185	arg1	field					166:170	the field	162:170	the field of anti-cancer	162:185	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	8	6	theme	immunosuppressive	1292:1308	arg1	alleviation					1310:1320	the immunosuppressive alleviation	1288:1320	the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy	1288:1407	APS combined with 5-FU could improve the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy.
31669273	6	7	theme	macrophages	1086:1096	arg1	phagocytosis					1059:1070	phagocytosis	1059:1070	phagocytosis of peritoneal macrophages in mice	1059:1104	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	5	8	theme	tissue	966:971	arg1	structure					923:931	the structure	919:931	the structure of the damaged thymus and spleen tissue	919:971	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	8	9	theme	immune	1333:1338	arg1	system					1340:1345	immune system	1333:1345	immune system	1333:1345	APS combined with 5-FU could improve the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy.
31669273	2	10	dep	in	352:353	arg1	vivo					355:358	vivo	355:358	vivo	355:358	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	3	11	theme	mediated	551:558	arg1	macrophages					560:570	APS mediated macrophages	547:570	APS mediated macrophages	547:570	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	7	12	theme	IFN-γ	1187:1191	arg1	expression					1157:1166	the expression	1153:1166	the expression of IL-2, TNF-α and IFN-γ in peripheral blood	1153:1211	Furthermore, APS was capable of up-regulating the expression of IL-2, TNF-α and IFN-γ in peripheral blood.
31669273	5	13	theme	damaged	940:946	arg1	tissue					966:971	the damaged thymus and spleen tissue	936:971	the damaged thymus and spleen tissue	936:971	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	2	14	theme	APS	291:293	arg1	composition					276:286	composition	276:286	composition	276:286	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	2	14	theme	APS	291:293	arg1	structure					262:270	structure	262:270	structure	262:270	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	7	15	from	expression	1157:1166	arg1	blood					1207:1211	peripheral blood	1196:1211	peripheral blood	1196:1211	Furthermore, APS was capable of up-regulating the expression of IL-2, TNF-α and IFN-γ in peripheral blood.
31669273	2	16	theme	host	412:415	arg1	system					424:429	host immune system	412:429	host immune system	412:429	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	1	17	theme	Astragalus	95:104	arg1	APS					122:124	APS	122:124	APS	122:124	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	1	17	theme	Astragalus	95:104	arg1	polysaccharide					106:119	Astragalus polysaccharide	95:119	Astragalus polysaccharide (APS)	95:125	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	2	18	theme	targeted	387:394	arg1	macrophages					396:406	APS targeted macrophages	383:406	APS targeted macrophages	383:406	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	7	19	theme	TNF-α	1177:1181	arg1	expression					1157:1166	the expression	1153:1166	the expression of IL-2, TNF-α and IFN-γ in peripheral blood	1153:1211	Furthermore, APS was capable of up-regulating the expression of IL-2, TNF-α and IFN-γ in peripheral blood.
31669273	3	20	contain	had	497:499	arg1	APS					493:495	APS	493:495	APS	493:495	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	20	contain	had	497:499	arg2	cytotoxicity					511:522	no direct cytotoxicity	501:522	no direct cytotoxicity against 4T1 cells	501:540	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	1	21	from	interests	149:157	arg1	field					166:170	the field	162:170	the field of anti-cancer	162:185	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	4	22	theme	cells	748:752	arg1	apoptosis					731:739	the apoptosis	727:739	the apoptosis of 4T1 cells	727:752	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	6	23	theme	peritoneal	1075:1084	arg1	macrophages					1086:1096	peritoneal macrophages	1075:1096	peritoneal macrophages in mice	1075:1104	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	2	24	theme	APS	383:385	arg1	macrophages					396:406	APS targeted macrophages	383:406	APS targeted macrophages	383:406	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	2	25	theme	immunoregulated	440:454	arg1	strategy					456:463	immunoregulated strategy	440:463	immunoregulated strategy	440:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	1	26	theme	killing	197:203	arg1	effect					205:210	direct killing effect	190:210	direct killing effect	190:210	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	7	27	theme	IL-2	1171:1174	arg1	expression					1157:1166	the expression	1153:1166	the expression of IL-2, TNF-α and IFN-γ in peripheral blood	1153:1211	Furthermore, APS was capable of up-regulating the expression of IL-2, TNF-α and IFN-γ in peripheral blood.
31669273	8	28	from	alleviation	1310:1320	arg1	system					1340:1345	immune system	1333:1345	immune system	1333:1345	APS combined with 5-FU could improve the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy.
31669273	2	29	theme	activity	371:378	arg1	evaluation					325:334	the evaluation	321:334	the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	321:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	6	30	from	phagocytosis	1059:1070	arg1	mice					1101:1104	mice	1101:1104	mice	1101:1104	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	3	31	theme	APS	547:549	arg1	macrophages					560:570	APS mediated macrophages	547:570	APS mediated macrophages	547:570	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	2	32	theme	immune	417:422	arg1	system					424:429	host immune system	412:429	host immune system	412:429	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	2	33	theme	system	424:429	arg1	activity					371:378	in vitro and in vivo anti-tumor activity	339:378	in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	339:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	3	34	theme	4T1	532:534	arg1	cells					536:540	4T1 cells	532:540	4T1 cells	532:540	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	2	35	dep	in	339:340	arg1	vitro					342:346	vitro	342:346	vitro	342:346	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	1	36	theme	improving	216:224	arg1	function					233:240	improving immune function	216:240	improving immune function	216:240	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	0	37	theme	astragalus	47:56	arg1	polysaccharides					58:72	astragalus polysaccharides	47:72	astragalus polysaccharides	47:72	Characterization and anti-tumor bioactivity of astragalus polysaccharides by immunomodulation.
31669273	2	38	theme	in	339:340	arg1	activity					371:378	in vitro and in vivo anti-tumor activity	339:378	in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	339:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	3	39	theme	cell	644:647	arg1	arrest					655:660	cell cycle arrest	644:660	cell cycle arrest (G2 phase)	644:671	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	39	theme	cell	644:647	arg1	phase					666:670	G2 phase	663:670	G2 phase	663:670	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	4	40	theme	mitochondrial	773:785	arg1	pathway					797:803	the mitochondrial apoptosis pathway	769:803	the mitochondrial apoptosis pathway	769:803	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	2	41	dep	structure	262:270	arg1	the					258:260	the	258:260	the	258:260	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	1	42	theme	immune	226:231	arg1	function					233:240	improving immune function	216:240	improving immune function	216:240	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	8	43	theme	anti-tumor	1255:1264	arg1	effect					1266:1271	the anti-tumor effect	1251:1271	the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy	1251:1407	APS combined with 5-FU could improve the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy.
31669273	2	44	theme	macrophages	396:406	arg1	activity					371:378	in vitro and in vivo anti-tumor activity	339:378	in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	339:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	5	45	theme	in	810:811	arg1	findings					818:825	The in vivo findings	806:825	The in vivo findings	806:825	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	5	46	theme	thymus	948:953	arg1	tissue					966:971	the damaged thymus and spleen tissue	936:971	the damaged thymus and spleen tissue	936:971	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	3	47	theme	apoptosis	682:690	arg1	induction					631:639	the induction	627:639	the induction of cell cycle arrest (G2 phase) and cell apoptosis	627:690	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	5	48	theme	spleen	893:898	arg1	index					900:904	spleen index	893:904	spleen index	893:904	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	0	49	theme	polysaccharides	58:72	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-tumor bioactivity of astragalus polysaccharides by immunomodulation.
31669273	0	49	theme	polysaccharides	58:72	arg1	bioactivity					32:42	anti-tumor bioactivity	21:42	anti-tumor bioactivity	21:42	Characterization and anti-tumor bioactivity of astragalus polysaccharides by immunomodulation.
31669273	3	50	theme	direct	504:509	arg1	cytotoxicity					511:522	no direct cytotoxicity	501:522	no direct cytotoxicity against 4T1 cells	501:540	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	1	51	theme	growing	141:147	arg1	interests					149:157	growing interests	141:157	growing interests in the field of anti-cancer	141:185	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	3	52	theme	4T1	614:616	arg1	cells					618:622	4T1 cells	614:622	4T1 cells	614:622	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	53	theme	G2	663:664	arg1	arrest					655:660	cell cycle arrest	644:660	cell cycle arrest (G2 phase)	644:671	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	53	theme	G2	663:664	arg1	phase					666:670	G2 phase	663:670	G2 phase	663:670	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	54	theme	cells	618:622	arg1	growth					604:609	the growth	600:609	the growth of 4T1 cells	600:622	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	4	55	theme	mediated	697:704	arg1	macrophages					706:716	APS mediated macrophages	693:716	APS mediated macrophages	693:716	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	3	56	theme	cycle	649:653	arg1	arrest					655:660	cell cycle arrest	644:660	cell cycle arrest (G2 phase)	644:671	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	3	56	theme	cycle	649:653	arg1	phase					666:670	G2 phase	663:670	G2 phase	663:670	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	4	57	theme	4T1	744:746	arg1	cells					748:752	4T1 cells	744:752	4T1 cells	744:752	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	6	58	from	macrophages	1086:1096	arg1	mice					1101:1104	mice	1101:1104	mice	1101:1104	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	4	59	theme	APS	693:695	arg1	macrophages					706:716	APS mediated macrophages	693:716	APS mediated macrophages	693:716	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	8	60	theme	5-FU	1325:1328	arg1	alleviation					1310:1320	the immunosuppressive alleviation	1288:1320	the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy	1288:1407	APS combined with 5-FU could improve the anti-tumor effect accompanied by the immunosuppressive alleviation of 5-FU on immune system, which may be suitable as an immune adjuvant for chemotherapy.
31669273	3	61	theme	arrest	655:660	arg1	induction					631:639	the induction	627:639	the induction of cell cycle arrest (G2 phase) and cell apoptosis	627:690	The results indicated that APS had no direct cytotoxicity against 4T1 cells, but APS mediated macrophages could significantly inhibit the growth of 4T1 cells by the induction of cell cycle arrest (G2 phase) and cell apoptosis.
31669273	4	62	theme	apoptosis	787:795	arg1	pathway					797:803	the mitochondrial apoptosis pathway	769:803	the mitochondrial apoptosis pathway	769:803	APS mediated macrophages promoted the apoptosis of 4T1 cells mainly through the mitochondrial apoptosis pathway.
31669273	6	63	theme	lymphocytes	1034:1044	arg1	proliferation					1010:1022	the proliferation	1006:1022	the proliferation of spleen lymphocytes	1006:1044	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	2	64	theme	anti-tumor	360:369	arg1	activity					371:378	in vitro and in vivo anti-tumor activity	339:378	in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy	339:463	In this study, the structure and composition of APS was determined, following the evaluation of in vitro and in vivo anti-tumor activity of APS targeted macrophages and host immune system based on immunoregulated strategy.
31669273	1	65	theme	direct	190:195	arg1	effect					205:210	direct killing effect	190:210	direct killing effect	190:210	Astragalus polysaccharide (APS) has attracted growing interests in the field of anti-cancer by direct killing effect and improving immune function.
31669273	5	66	theme	spleen	959:964	arg1	tissue					966:971	the damaged thymus and spleen tissue	936:971	the damaged thymus and spleen tissue	936:971	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
31669273	0	67	theme	anti-tumor	21:30	arg1	bioactivity					32:42	anti-tumor bioactivity	21:42	anti-tumor bioactivity	21:42	Characterization and anti-tumor bioactivity of astragalus polysaccharides by immunomodulation.
31669273	6	68	from	mice	1101:1104	arg1	phagocytosis					1059:1070	phagocytosis	1059:1070	phagocytosis of peritoneal macrophages in mice	1059:1104	APS could significantly enhance the proliferation of spleen lymphocytes and increase phagocytosis of peritoneal macrophages in mice.
31669273	5	69	theme	thymus	876:881	arg1	index					883:887	the thymus index	872:887	the thymus index	872:887	The in vivo findings demonstrated that APS could markedly improve the thymus index and spleen index, and restore the structure of the damaged thymus and spleen tissue.
30773292	7	0	theme	ecophysiological	1274:1289	arg1	factor					1291:1296	an important ecophysiological factor	1261:1296	an important ecophysiological factor in response to environmental alterations	1261:1337	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	4	1	theme	considerable	508:519	arg1	number					521:526	a considerable number	506:526	a considerable number of carbohydrate-active enzymes	506:557	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	5	2	theme	certain	715:721	arg1	capacity					723:730	a certain capacity	713:730	a certain capacity for carbohydrate utilization	713:759	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	6	3	contain	possessed	875:883	arg2	cycling					909:915	potential intracellular cycling	885:915	potential intracellular cycling	885:915	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	3	contain	possessed	875:883	arg1	RN62					870:873	RN62	870:873	RN62	870:873	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	3	contain	possessed	875:883	arg2	strategy					970:977	a strategy	968:977	a strategy for carbon storage and consumption	968:1012	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	5	4	theme	hadal	774:778	arg1	ocean					780:784	the hadal ocean	770:784	the hadal ocean	770:784	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	6	5	theme	nutrient	1029:1036	arg1	pulse					1038:1042	nutrient pulse	1029:1042	nutrient pulse	1029:1042	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	8	6	theme	pressure/low	1563:1574	arg1	temperature					1576:1586	high pressure/low temperature	1558:1586	high pressure/low temperature	1558:1586	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	4	7	theme	carbohydrate-active	531:549	arg1	enzymes					551:557	carbohydrate-active enzymes	531:557	carbohydrate-active enzymes	531:557	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	7	8	theme	dissimilarities	1114:1128	arg1	discovery					1074:1082	the discovery	1070:1082	the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia	1070:1149	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	5	9	theme	family	620:625	arg1	proteins					630:637	GH family 13 proteins	617:637	GH family 13 proteins	617:637	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	7	10	theme	glycoside	1094:1102	arg1	dissimilarities					1114:1128	higher glycoside hydrolase dissimilarities	1087:1128	higher glycoside hydrolase dissimilarities	1087:1128	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	3	11	theme	strain	375:380	arg1	RN62					400:403	the flavobacterial strain Euzebyella marina RN62	356:403	the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance	356:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	7	12	from	variation	1203:1211	arg1	traits					1251:1256	polysaccharide utilization related traits	1216:1256	polysaccharide utilization related traits	1216:1256	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	12	from	variation	1203:1211	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	12	from	variation	1203:1211	arg1	waters					1389:1394	hadal waters	1383:1394	hadal waters	1383:1394	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	6	13	dep	pulse	1038:1042	arg1	response					1017:1024	response	1017:1024	response	1017:1024	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	14	theme	pathway	940:946	arg1	strategy					970:977	a strategy	968:977	a strategy for carbon storage and consumption	968:1012	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	14	theme	pathway	940:946	arg1	cycling					909:915	potential intracellular cycling	885:915	potential intracellular cycling	885:915	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	3	15	theme	Euzebyella	382:391	arg1	RN62					400:403	the flavobacterial strain Euzebyella marina RN62	356:403	the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance	356:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	4	16	theme	RN62	491:494	arg1	genome					481:486	The complete genome	468:486	The complete genome of RN62	468:494	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	8	17	theme	toxin	1422:1426	arg1	genes					1485:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	5	18	theme	proteins	630:637	arg1	predominance					601:612	a predominance	599:612	a predominance of GH family 13 proteins	599:637	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	5	19	theme	distinct	823:830	arg1	reservoir					813:821	an organic matter reservoir	795:821	an organic matter reservoir distinct from the surface ocean	795:853	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	4	20	contain	possessed	496:504	arg2	number					521:526	a considerable number	506:526	a considerable number of carbohydrate-active enzymes	506:557	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	4	20	contain	possessed	496:504	arg1	genome					481:486	The complete genome	468:486	The complete genome of RN62	468:494	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	8	21	theme	hadal	1530:1534	arg1	temperature					1576:1586	high pressure/low temperature	1558:1586	high pressure/low temperature	1558:1586	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	8	21	theme	hadal	1530:1534	arg1	conditions					1536:1545	hadal conditions	1530:1545	hadal conditions	1530:1545	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	7	22	from	factor	1291:1296	arg1	response					1301:1308	response	1301:1308	response to environmental alterations	1301:1337	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	3	23	theme	low	453:455	arg1	abundance					457:465	low abundance	453:465	low abundance	453:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	3	24	theme	flavobacterial	360:373	arg1	RN62					400:403	the flavobacterial strain Euzebyella marina RN62	356:403	the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance	356:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	0	25	theme	Flavobacteriia	39:52	arg1	bacterium					54:62	a Flavobacteriia bacterium	37:62	a Flavobacteriia bacterium	37:62	Carbohydrate catabolic capability of a Flavobacteriia bacterium isolated from hadal water.
30773292	1	26	from	environments	134:145	arg1	abundant					110:117	abundant	110:117	abundant	110:117	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	7	27	theme	related	1243:1249	arg1	traits					1251:1256	polysaccharide utilization related traits	1216:1256	polysaccharide utilization related traits	1216:1256	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	27	theme	related	1243:1249	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	0	28	theme	catabolic	13:21	arg1	capability					23:32	Carbohydrate catabolic capability	0:32	Carbohydrate catabolic capability of a Flavobacteriia bacterium	0:62	Carbohydrate catabolic capability of a Flavobacteriia bacterium isolated from hadal water.
30773292	5	29	theme	related	659:665	arg1	relatives					667:675	closely related relatives	651:675	closely related relatives	651:675	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	8	30	theme	transcription	1439:1451	arg1	genes					1485:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	5	31	from	ocean	849:853	arg1	distinct					823:830	distinct	823:830	distinct	823:830	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	0	32	theme	Carbohydrate	0:11	arg1	capability					23:32	Carbohydrate catabolic capability	0:32	Carbohydrate catabolic capability of a Flavobacteriia bacterium	0:62	Carbohydrate catabolic capability of a Flavobacteriia bacterium isolated from hadal water.
30773292	7	33	theme	higher	1087:1092	arg1	dissimilarities					1114:1128	higher glycoside hydrolase dissimilarities	1087:1128	higher glycoside hydrolase dissimilarities	1087:1128	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	0	34	theme	bacterium	54:62	arg1	capability					23:32	Carbohydrate catabolic capability	0:32	Carbohydrate catabolic capability of a Flavobacteriia bacterium	0:62	Carbohydrate catabolic capability of a Flavobacteriia bacterium isolated from hadal water.
30773292	3	35	theme	marina	393:398	arg1	RN62					400:403	the flavobacterial strain Euzebyella marina RN62	356:403	the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance	356:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	5	36	theme	organic	798:804	arg1	reservoir					813:821	an organic matter reservoir	795:821	an organic matter reservoir distinct from the surface ocean	795:853	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	5	37	theme	GH	617:618	arg1	proteins					630:637	GH family 13 proteins	617:637	GH family 13 proteins	617:637	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	8	38	theme	signal	1457:1462	arg1	transduction					1464:1475	signal transduction	1457:1475	signal transduction	1457:1475	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	7	39	theme	polysaccharide	1216:1229	arg1	traits					1251:1256	polysaccharide utilization related traits	1216:1256	polysaccharide utilization related traits	1216:1256	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	39	theme	polysaccharide	1216:1229	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	40	theme	hydrolase	1104:1112	arg1	dissimilarities					1114:1128	higher glycoside hydrolase dissimilarities	1087:1128	higher glycoside hydrolase dissimilarities	1087:1128	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	41	theme	environmental	1313:1325	arg1	alterations					1327:1337	environmental alterations	1313:1337	environmental alterations	1313:1337	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	42	theme	hadal	1383:1387	arg1	waters					1389:1394	hadal waters	1383:1394	hadal waters	1383:1394	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	3	43	theme	Trench	431:436	arg1	water					444:448	the Mariana Trench hadal water	419:448	the Mariana Trench hadal water in low abundance	419:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	6	44	theme	carbon	983:988	arg1	storage					990:996	carbon storage	983:996	carbon storage	983:996	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	5	45	theme	carbohydrate	736:747	arg1	utilization					749:759	carbohydrate utilization	736:759	carbohydrate utilization	736:759	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	6	46	theme	intracellular	895:907	arg1	strategy					970:977	a strategy	968:977	a strategy for carbon storage and consumption	968:1012	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	46	theme	intracellular	895:907	arg1	cycling					909:915	potential intracellular cycling	885:915	potential intracellular cycling	885:915	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	8	47	theme	related	1477:1483	arg1	genes					1485:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	7	48	theme	organic	1365:1371	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	3	49	theme	hadal	438:442	arg1	water					444:448	the Mariana Trench hadal water	419:448	the Mariana Trench hadal water in low abundance	419:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	5	50	theme	matter	806:811	arg1	reservoir					813:821	an organic matter reservoir	795:821	an organic matter reservoir distinct from the surface ocean	795:853	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	6	51	theme	potential	885:893	arg1	strategy					970:977	a strategy	968:977	a strategy for carbon storage and consumption	968:1012	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	6	51	theme	potential	885:893	arg1	cycling					909:915	potential intracellular cycling	885:915	potential intracellular cycling	885:915	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	2	52	theme	flavobacterial	229:242	arg1	population					244:253	this flavobacterial population	224:253	this flavobacterial population	224:253	However, it is unclear how this flavobacterial population adapts to hadal conditions.
30773292	5	53	theme	surface	841:847	arg1	ocean					849:853	the surface ocean	837:853	the surface ocean	837:853	There was a predominance of GH family 13 proteins compared to closely related relatives, suggesting that RN62 has preserved a certain capacity for carbohydrate utilization and that the hadal ocean may hold an organic matter reservoir distinct from the surface ocean.
30773292	4	54	theme	enzymes	551:557	arg1	number					521:526	a considerable number	506:526	a considerable number of carbohydrate-active enzymes	506:557	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	3	55	theme	comparative	308:318	arg1	analyses					328:335	extensive comparative genomic analyses	298:335	extensive comparative genomic analyses	298:335	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	4	56	theme	complete	472:479	arg1	genome					481:486	The complete genome	468:486	The complete genome of RN62	468:494	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	1	57	theme	many	122:125	arg1	environments					134:145	many marine environments	122:145	many marine environments including hadal waters	122:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	1	57	theme	many	122:125	arg1	waters					163:168	hadal waters	157:168	hadal waters	157:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	3	58	theme	Mariana	423:429	arg1	water					444:448	the Mariana Trench hadal water	419:448	the Mariana Trench hadal water in low abundance	419:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	4	59	theme	different	566:574	arg1	composition					576:586	a different composition	564:586	a different composition	564:586	The complete genome of RN62 possessed a considerable number of carbohydrate-active enzymes with a different composition.
30773292	3	60	theme	genomic	320:326	arg1	analyses					328:335	extensive comparative genomic analyses	298:335	extensive comparative genomic analyses	298:335	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	6	61	theme	glycogen/starch	924:938	arg1	pathway					940:946	the glycogen/starch pathway	920:946	the glycogen/starch pathway	920:946	Additionally, RN62 possessed potential intracellular cycling of the glycogen/starch pathway, which may serve as a strategy for carbon storage and consumption in response to nutrient pulse and starvation.
30773292	7	62	theme	decreased	1348:1356	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	1	63	theme	marine	127:132	arg1	environments					134:145	many marine environments	122:145	many marine environments including hadal waters	122:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	1	63	theme	marine	127:132	arg1	waters					163:168	hadal waters	157:168	hadal waters	157:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	1	64	from	abundant	110:117	arg1	environments					134:145	many marine environments	122:145	many marine environments including hadal waters	122:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	1	64	from	abundant	110:117	arg1	waters					163:168	hadal waters	157:168	hadal waters	157:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	3	65	attach	isolated	405:412	arg1	water					444:448	the Mariana Trench hadal water	419:448	the Mariana Trench hadal water in low abundance	419:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	3	65	attach	isolated	405:412	arg2	RN62					400:403	the flavobacterial strain Euzebyella marina RN62	356:403	the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance	356:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	8	66	theme	transduction	1464:1475	arg1	genes					1485:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	8	67	theme	exporting	1428:1436	arg1	toxin					1422:1426	abundant toxin exporting	1413:1436	abundant toxin exporting	1413:1436	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	3	68	theme	extensive	298:306	arg1	analyses					328:335	extensive comparative genomic analyses	298:335	extensive comparative genomic analyses	298:335	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	8	69	theme	high	1558:1561	arg1	temperature					1576:1586	high pressure/low temperature	1558:1586	high pressure/low temperature	1558:1586	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	2	70	theme	hadal	265:269	arg1	conditions					271:280	hadal conditions	265:280	hadal conditions	265:280	However, it is unclear how this flavobacterial population adapts to hadal conditions.
30773292	3	71	from	water	444:448	arg1	abundance					457:465	low abundance	453:465	low abundance	453:465	In this study, extensive comparative genomic analyses were performed for the flavobacterial strain Euzebyella marina RN62 isolated from the Mariana Trench hadal water in low abundance.
30773292	8	72	from	presence	1401:1408	arg1	RN62					1494:1497	RN62	1494:1497	RN62	1494:1497	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	0	73	theme	hadal	78:82	arg1	water					84:88	hadal water	78:88	hadal water	78:88	Carbohydrate catabolic capability of a Flavobacteriia bacterium isolated from hadal water.
30773292	8	74	theme	abundant	1413:1420	arg1	toxin					1422:1426	abundant toxin exporting	1413:1436	abundant toxin exporting	1413:1436	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	1	75	theme	hadal	157:161	arg1	waters					163:168	hadal waters	157:168	hadal waters	157:168	Flavobacteriia are abundant in many marine environments including hadal waters, as demonstrated recently.
30773292	8	76	attach	presence	1401:1408	arg1	RN62					1494:1497	RN62	1494:1497	RN62	1494:1497	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	8	76	attach	presence	1401:1408	arg2	genes					1485:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	abundant toxin exporting, transcription and signal transduction related genes	1413:1489	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	7	77	theme	labile	1358:1363	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	78	theme	utilization	1231:1241	arg1	traits					1251:1256	polysaccharide utilization related traits	1216:1256	polysaccharide utilization related traits	1216:1256	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	7	78	theme	utilization	1231:1241	arg1	carbon					1373:1378	decreased labile organic carbon	1348:1378	decreased labile organic carbon	1348:1378	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
30773292	8	79	theme	genes	1485:1489	arg1	presence					1401:1408	The presence	1397:1408	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62	1397:1497	The presence of abundant toxin exporting, transcription and signal transduction related genes in RN62 may further help to survive in hadal conditions, including high pressure/low temperature.
30773292	7	80	theme	important	1264:1272	arg1	factor					1291:1296	an important ecophysiological factor	1261:1296	an important ecophysiological factor in response to environmental alterations	1261:1337	Moreover, the discovery of higher glycoside hydrolase dissimilarities among Flavobacteriia, compared to peptidases and transporters, suggested variation in polysaccharide utilization related traits as an important ecophysiological factor in response to environmental alterations, such as decreased labile organic carbon in hadal waters.
31809910	7	0	theme	κ-carrageenan	1147:1159	arg1	solutions					1161:1169	aqueous κ-carrageenan solutions	1139:1169	aqueous κ-carrageenan solutions	1139:1169	These materials are prepared by cooling aqueous κ-carrageenan solutions at 4.0 and 5.0 wt%.
31809910	11	1	theme	distilled	1631:1639	arg1	water					1641:1645	distilled water	1631:1645	distilled water	1631:1645	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	1	2	theme	kappa-carrageenan	181:197	arg1	solutions					215:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	10	3	contain	have	1458:1461	arg1	hydrogels					1448:1456	hydrogels	1448:1456	hydrogels	1448:1456	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	10	3	contain	have	1458:1461	arg2	surfaces					1481:1488	porous and smooth surfaces	1463:1488	porous and smooth surfaces	1463:1488	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	0	4	theme	superabsorbent	73:86	arg1	hydrogels					88:96	its durable superabsorbent hydrogels	61:96	its durable superabsorbent hydrogels	61:96	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	7	5	theme	aqueous	1139:1145	arg1	solutions					1161:1169	aqueous κ-carrageenan solutions	1139:1169	aqueous κ-carrageenan solutions	1139:1169	These materials are prepared by cooling aqueous κ-carrageenan solutions at 4.0 and 5.0 wt%.
31809910	10	6	theme	porous	1463:1468	arg1	surfaces					1481:1488	porous and smooth surfaces	1463:1488	porous and smooth surfaces	1463:1488	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	5	7	theme	sample	937:942	arg1	surfaces					944:951	the sample surfaces	933:951	the sample surfaces	933:951	XPS supports the occurrence of a low protein content onto the sample surfaces, as well.
31809910	3	8	theme	absorption	532:541	arg1	spectrometry					543:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	9	9	from	content	1374:1380	arg1	sample					1400:1405	the commercial sample	1385:1405	the commercial sample	1385:1405	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	6	10	theme	commercial	1009:1018	arg1	κ-carrageenan					1020:1032	the commercial κ-carrageenan	1005:1032	the commercial κ-carrageenan	1005:1032	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	12	11	theme	soil	1749:1752	arg1	conditioners					1754:1765	soil conditioners	1749:1765	soil conditioners	1749:1765	These hydrogels are superabsorbent materials and can be applied in agriculture as soil conditioners.
31809910	12	11	theme	soil	1749:1752	arg1	hydrogels					1673:1681	These hydrogels	1667:1681	These hydrogels	1667:1681	These hydrogels are superabsorbent materials and can be applied in agriculture as soil conditioners.
31809910	11	12	theme	disintegration/dissolution	1547:1572	arg1	test					1574:1577	the disintegration/dissolution test	1543:1577	the disintegration/dissolution test	1543:1577	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	3	13	theme	commercial	571:580	arg1	κ-carrageenan					582:594	the commercial κ-carrageenan	567:594	the commercial κ-carrageenan	567:594	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	4	14	theme	photoelectron	710:722	arg1	XPS					738:740	XPS	738:740	XPS	738:740	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	14	theme	photoelectron	710:722	arg1	spectroscopy					724:735	The X-ray photoelectron spectroscopy	700:735	The X-ray photoelectron spectroscopy (XPS)	700:741	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	9	15	theme	commercial	1389:1398	arg1	sample					1400:1405	the commercial sample	1385:1405	the commercial sample	1385:1405	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	4	16	theme	atoms	800:804	arg1	presence					757:764	the presence	753:764	the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces	753:872	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	11	17	dep	swell	1511:1515	arg1	while					1537:1541	while	1537:1541	while	1537:1541	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	3	18	theme	atomic	525:530	arg1	spectrometry					543:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	12	19	theme	superabsorbent	1687:1700	arg1	hydrogels					1673:1681	These hydrogels	1667:1681	These hydrogels	1667:1681	These hydrogels are superabsorbent materials and can be applied in agriculture as soil conditioners.
31809910	12	19	theme	superabsorbent	1687:1700	arg1	materials					1702:1710	superabsorbent materials	1687:1710	superabsorbent materials	1687:1710	These hydrogels are superabsorbent materials and can be applied in agriculture as soil conditioners.
31809910	8	20	theme	gelation	1195:1202	arg1	temperature					1204:1214	The gelation temperature	1191:1214	The gelation temperature	1191:1214	The gelation temperature is determined by measuring G' &G″ as a function of the temperature.
31809910	9	21	theme	κ-carrageenan	1321:1333	arg1	concentration					1335:1347	the κ-carrageenan concentration	1317:1347	the κ-carrageenan concentration	1317:1347	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	4	22	theme	as-received	813:823	arg1	κ-carrageenan					825:837	as-received κ-carrageenan	813:837	as-received κ-carrageenan	813:837	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	5	23	theme	protein	912:918	arg1	content					920:926	a low protein content	906:926	a low protein content onto the sample surfaces	906:951	XPS supports the occurrence of a low protein content onto the sample surfaces, as well.
31809910	1	24	theme	κ-carrageenan	200:212	arg1	solutions					215:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	0	25	theme	food	41:44	arg1	ingredient					46:55	a commercial κ-carrageenan food ingredient	14:55	a commercial κ-carrageenan food ingredient	14:55	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	4	26	theme	X-ray	704:708	arg1	XPS					738:740	XPS	738:740	XPS	738:740	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	26	theme	X-ray	704:708	arg1	spectroscopy					724:735	The X-ray photoelectron spectroscopy	700:735	The X-ray photoelectron spectroscopy (XPS)	700:741	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	2	27	theme	κ-carrageenan	475:487	arg1	properties					439:448	the properties	435:448	the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco	435:512	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	2	28	used	used	366:369	arg2	hydrogels					346:354	The κ-carrageenan hydrogels	328:354	The κ-carrageenan hydrogels	328:354	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	3	29	theme	high	608:611	arg1	content					613:619	a high content	606:619	a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1)	606:697	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	6	30	theme	hydrogels	1088:1096	arg1	preparation					1065:1075	the preparation	1061:1075	the preparation of durable hydrogels	1061:1096	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	3	31	dep	ions	633:636	arg1	ions					633:636	metallic ions	624:636	metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1)	624:697	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	31	dep	ions	633:636	arg1	Ca2+ = 12.5 g kg-1					679:696	Ca2+ = 12.5 g kg-1	679:696	Ca2+ = 12.5 g kg-1	679:696	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	31	dep	ions	633:636	arg1	Na+ = 6.3 g kg-1					658:673	Na+ = 6.3 g kg-1	658:673	Na+ = 6.3 g kg-1	658:673	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	31	dep	ions	633:636	arg1	K+ = 216.1 g kg-1					639:655	K+ = 216.1 g kg-1	639:655	K+ = 216.1 g kg-1	639:655	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	2	32	theme	GENUGEL®	466:473	arg1	κ-carrageenan					475:487	a commercial GENUGEL® κ-carrageenan	453:487	a commercial GENUGEL® κ-carrageenan produced by the CP Kelco	453:512	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	11	33	from	stability	1618:1626	arg1	water					1641:1645	distilled water	1631:1645	distilled water	1631:1645	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	9	34	from	concentration	1335:1347	arg1	sample					1400:1405	the commercial sample	1385:1405	the commercial sample	1385:1405	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	11	35	dep	3100	1530:1533	arg1	to					1527:1528	to	1527:1528	to	1527:1528	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	1	36	theme	Physical	99:106	arg1	hydrogels					132:140	Physical kappa-carrageenan-based hydrogels	99:140	Physical kappa-carrageenan-based hydrogels	99:140	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	5	37	theme	content	920:926	arg1	occurrence					892:901	the occurrence	888:901	the occurrence of a low protein content onto the sample surfaces	888:951	XPS supports the occurrence of a low protein content onto the sample surfaces, as well.
31809910	0	38	theme	commercial	16:25	arg1	ingredient					46:55	a commercial κ-carrageenan food ingredient	14:55	a commercial κ-carrageenan food ingredient	14:55	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	1	39	theme	other	305:309	arg1	polysaccharides					311:325	other polysaccharides	305:325	other polysaccharides	305:325	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	10	40	theme	electron	1417:1424	arg1	microscopy					1426:1435	Scanning electron microscopy	1408:1435	Scanning electron microscopy	1408:1435	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	2	41	theme	κ-carrageenan	332:344	arg1	hydrogels					346:354	The κ-carrageenan hydrogels	328:354	The κ-carrageenan hydrogels	328:354	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	10	42	theme	Scanning	1408:1415	arg1	microscopy					1426:1435	Scanning electron microscopy	1408:1435	Scanning electron microscopy	1408:1435	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	8	43	theme	temperature	1271:1281	arg1	function					1255:1262	a function	1253:1262	a function of the temperature	1253:1281	The gelation temperature is determined by measuring G' &G″ as a function of the temperature.
31809910	8	43	theme	temperature	1271:1281	arg1	&G″					1246:1248	G' &G″	1243:1248	G' &G″	1243:1248	The gelation temperature is determined by measuring G' &G″ as a function of the temperature.
31809910	4	44	theme	calcium	777:783	arg1	atoms					800:804	sodium, calcium, and potassium atoms	769:804	sodium, calcium, and potassium atoms	769:804	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	45	theme	potassium	790:798	arg1	atoms					800:804	sodium, calcium, and potassium atoms	769:804	sodium, calcium, and potassium atoms	769:804	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	5	46	theme	low	908:910	arg1	content					920:926	a low protein content	906:926	a low protein content onto the sample surfaces	906:951	XPS supports the occurrence of a low protein content onto the sample surfaces, as well.
31809910	2	47	theme	CP	505:506	arg1	Kelco					508:512	the CP Kelco	501:512	the CP Kelco	501:512	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	3	48	theme	ions	633:636	arg1	content					613:619	a high content	606:619	a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1)	606:697	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	6	49	theme	durable	1080:1086	arg1	hydrogels					1088:1096	durable hydrogels	1080:1096	durable hydrogels	1080:1096	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	0	50	theme	κ-carrageenan	27:39	arg1	ingredient					46:55	a commercial κ-carrageenan food ingredient	14:55	a commercial κ-carrageenan food ingredient	14:55	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	9	51	theme	metallic	1365:1372	arg1	content					1374:1380	the metallic content	1361:1380	the metallic content in the commercial sample	1361:1405	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	2	52	theme	commercial	455:464	arg1	κ-carrageenan					475:487	a commercial GENUGEL® κ-carrageenan	453:487	a commercial GENUGEL® κ-carrageenan produced by the CP Kelco	453:512	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	11	53	theme	high	1613:1616	arg1	stability					1618:1626	high stability	1613:1626	high stability in distilled water	1613:1645	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	1	54	theme	metallic	244:251	arg1	ions					253:256	metallic ions	244:256	metallic ions	244:256	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	2	55	theme	technological	375:387	arg1	purposes					389:396	technological purposes	375:396	technological purposes	375:396	The κ-carrageenan hydrogels have been used for technological purposes; however, there are no reports about the properties of a commercial GENUGEL® κ-carrageenan produced by the CP Kelco.
31809910	0	56	theme	ingredient	46:55	arg1	Properties					0:9	Properties	0:9	Properties of a commercial κ-carrageenan food ingredient	0:55	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	0	56	theme	ingredient	46:55	arg1	hydrogels					88:96	its durable superabsorbent hydrogels	61:96	its durable superabsorbent hydrogels	61:96	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	6	57	theme	metallic	967:974	arg1	level					976:980	The metallic level	963:980	The metallic level (especially for K+) in the commercial κ-carrageenan	963:1032	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	9	58	theme	gelation	1288:1295	arg1	behavior					1297:1304	The gelation behavior	1284:1304	The gelation behavior	1284:1304	The gelation behavior depends on the κ-carrageenan concentration, as well as the metallic content in the commercial sample.
31809910	3	59	theme	metallic	624:631	arg1	ions					633:636	metallic ions	624:636	metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1)	624:697	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	59	theme	metallic	624:631	arg1	Ca2+ = 12.5 g kg-1					679:696	Ca2+ = 12.5 g kg-1	679:696	Ca2+ = 12.5 g kg-1	679:696	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	59	theme	metallic	624:631	arg1	Na+ = 6.3 g kg-1					658:673	Na+ = 6.3 g kg-1	658:673	Na+ = 6.3 g kg-1	658:673	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	59	theme	metallic	624:631	arg1	K+ = 216.1 g kg-1					639:655	K+ = 216.1 g kg-1	639:655	K+ = 216.1 g kg-1	639:655	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	3	60	theme	flame	519:523	arg1	spectrometry					543:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry	515:554	The flame atomic absorption spectrometry shows that the commercial κ-carrageenan comprises a high content of metallic ions (K+ = 216.1 g kg-1, Na+ = 6.3 g kg-1 and Ca2+ = 12.5 g kg-1).
31809910	4	61	theme	hydrogel	856:863	arg1	surfaces					865:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	1	62	theme	kappa-carrageenan-based	108:130	arg1	hydrogels					132:140	Physical kappa-carrageenan-based hydrogels	99:140	Physical kappa-carrageenan-based hydrogels	99:140	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	1	63	theme	ions	253:256	arg1	presence					232:239	the presence	228:239	the presence of metallic ions	228:256	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	6	64	from	level	976:980	arg1	κ-carrageenan					1020:1032	the commercial κ-carrageenan	1005:1032	the commercial κ-carrageenan	1005:1032	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	4	65	theme	κ-carrageenan	825:837	arg1	surfaces					865:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	66	theme	sodium	769:774	arg1	atoms					800:804	sodium, calcium, and potassium atoms	769:804	sodium, calcium, and potassium atoms	769:804	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	11	67	theme	dried	1495:1499	arg1	materials					1501:1509	The dried materials	1491:1509	The dried materials	1491:1509	The dried materials swell from 2400 to 3100%, while the disintegration/dissolution test confirms that the samples present high stability in distilled water throughout 14 days.
31809910	6	68	theme	essential	1043:1051	arg1	role					1053:1056	an essential role	1040:1056	an essential role	1040:1056	The metallic level (especially for K+) in the commercial κ-carrageenan plays an essential role in the preparation of durable hydrogels.
31809910	0	69	theme	durable	65:71	arg1	hydrogels					88:96	its durable superabsorbent hydrogels	61:96	its durable superabsorbent hydrogels	61:96	Properties of a commercial κ-carrageenan food ingredient and its durable superabsorbent hydrogels.
31809910	10	70	theme	smooth	1474:1479	arg1	surfaces					1481:1488	porous and smooth surfaces	1463:1488	porous and smooth surfaces	1463:1488	Scanning electron microscopy shows that hydrogels have porous and smooth surfaces.
31809910	4	71	theme	physical	847:854	arg1	hydrogel					856:863	its physical hydrogel	843:863	its physical hydrogel	843:863	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	1	72	theme	dilute	166:171	arg1	solutions					215:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
31809910	4	73	from	presence	757:764	arg1	surfaces					865:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	74	attach	presence	757:764	arg1	surfaces					865:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	the as-received κ-carrageenan and its physical hydrogel surfaces	809:872	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	4	74	attach	presence	757:764	arg2	atoms					800:804	sodium, calcium, and potassium atoms	769:804	sodium, calcium, and potassium atoms	769:804	The X-ray photoelectron spectroscopy (XPS) indicates the presence of sodium, calcium, and potassium atoms on the as-received κ-carrageenan and its physical hydrogel surfaces.
31809910	1	75	theme	aqueous	173:179	arg1	solutions					215:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	dilute aqueous kappa-carrageenan (κ-carrageenan) solutions	166:223	Physical kappa-carrageenan-based hydrogels are often prepared from dilute aqueous kappa-carrageenan (κ-carrageenan) solutions at the presence of metallic ions or by mixing these solutions with proteins and other polysaccharides.
30658251	4	0	theme	lipooligosaccharide	453:471	arg1	fraction					479:486	the lipooligosaccharide (LOS) fraction	449:486	the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production	449:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	6	1	theme	1 → 6	930:934	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	1	theme	1 → 6	930:934	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	1	2	theme	bacterial	150:158	arg1	surface					165:171	the Gram-negative bacterial cell surface	132:171	the Gram-negative bacterial cell surface	132:171	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	8	3	contain	carried	1311:1317	arg1	GlcN-1					1271:1276	GlcN-1	1271:1276	GlcN-1	1271:1276	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	3	contain	carried	1311:1317	arg1	N2					1292:1293	3-OH-14:0 at N2	1279:1293	3-OH-14:0 at N2 of GlcN-2	1279:1303	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	3	contain	carried	1311:1317	arg2	Δ7					1332:1333	another 14:1 Δ7	1319:1333	another 14:1 Δ7 on its β-hydroxyl group	1319:1357	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	4	4	theme	IL-8	550:553	arg1	production					536:545	production	536:545	production of IL-8	536:553	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	0	5	from	analysis	11:18	arg1	Rhodobacter					62:72	Rhodobacter	62:72	Rhodobacter	62:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	8	6	theme	3-OH-10:0 at	1391:1402	arg1	O3					1404:1405	3-OH-10:0 at O3	1391:1405	3-OH-10:0 at O3 of both GlcN	1391:1418	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	1	7	theme	cell	160:163	arg1	surface					165:171	the Gram-negative bacterial cell surface	132:171	the Gram-negative bacterial cell surface	132:171	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	6	8	theme	KDOα	900:903	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	8	theme	KDOα	900:903	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	5	9	theme	resonance	695:703	arg1	spectroscopy					705:716	nuclear magnetic resonance spectroscopy	678:716	nuclear magnetic resonance spectroscopy	678:716	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	7	10	with	extension	1128:1136	arg1	hexoses					1143:1149	hexoses	1143:1149	hexoses	1143:1149	The structure was consistent with one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses.
30658251	8	11	theme	C14	1215:1217	arg1	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	11	theme	C14	1215:1217	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	11	theme	C14	1215:1217	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	12	theme	GlcN-2	1298:1303	arg1	GlcN-1					1271:1276	GlcN-1	1271:1276	GlcN-1	1271:1276	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	12	theme	GlcN-2	1298:1303	arg1	N2					1292:1293	3-OH-14:0 at N2	1279:1293	3-OH-14:0 at N2 of GlcN-2	1279:1303	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	6	13	theme	1 → 4	858:862	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	13	theme	1 → 4	858:862	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	5	14	theme	molecular	627:635	arg1	structure					637:645	their molecular structure	621:645	their molecular structure	621:645	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	2	15	theme	Rhodobacter	271:281	arg1	sp					283:284	Rhodobacter sp	271:284	Rhodobacter sp.	271:285	Rhodobacter sp.
30658251	1	16	theme	4-MD-2	255:260	arg1	complex					262:268	the Toll-like receptor (TLR) 4-MD-2 complex	226:268	the Toll-like receptor (TLR) 4-MD-2 complex	226:268	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	6	17	theme	NMR	974:976	arg1	methods					978:984	NMR methods	974:984	NMR methods	974:984	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	8	18	theme	acyl	1225:1228	arg1	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	18	theme	acyl	1225:1228	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	18	theme	acyl	1225:1228	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	1	19	theme	surface	165:171	arg1	components					118:127	components	118:127	components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex	118:268	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	1	19	theme	surface	165:171	arg1	Lipopolysaccharides					88:106	Lipopolysaccharides	88:106	Lipopolysaccharides (LPS)	88:112	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	3	20	theme	endotoxic	331:339	arg1	activity					341:348	endotoxic activity	331:348	endotoxic activity	331:348	have been reported to produce LPS that lack endotoxic activity, and instead act as antagonists of other endotoxins.
30658251	6	21	theme	Glcα	853:856	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	21	theme	Glcα	853:856	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	0	22	theme	lipooligosaccharide	31:49	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter	0:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	7	23	theme	MS	1051:1052	arg1	analysis					1054:1061	The MS analysis	1047:1061	The MS analysis	1047:1061	The structure was consistent with one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses.
30658251	7	24	with	consistent	1005:1014	arg1	one					1021:1023	one	1021:1023	one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses	1021:1149	The structure was consistent with one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses.
30658251	6	25	theme	KDOα	876:879	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	25	theme	KDOα	876:879	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	8	26	from	composition	1161:1171	arg1	A					1182:1182	lipid A	1176:1182	lipid A	1176:1182	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	27	theme	C10	1368:1370	arg1	chains					1383:1388	two C10 fatty acyl chains	1364:1388	two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN)	1364:1419	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	28	theme	GlcN	1415:1418	arg1	O3					1404:1405	3-OH-10:0 at O3	1391:1405	3-OH-10:0 at O3 of both GlcN	1391:1418	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	0	29	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter	0:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	6	30	theme	1 → 5	893:897	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	30	theme	1 → 5	893:897	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	8	31	theme	14:1	1327:1330	arg1	Δ7					1332:1333	another 14:1 Δ7	1319:1333	another 14:1 Δ7 on its β-hydroxyl group	1319:1357	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	6	32	theme	glycan	835:840	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	32	theme	glycan	835:840	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	5	33	theme	glucuronic	793:802	arg1	acid					804:807	glucuronic acid	793:807	glucuronic acid but not heptoses	793:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	8	34	theme	acyl	1378:1381	arg1	chains					1383:1388	two C10 fatty acyl chains	1364:1388	two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN)	1364:1419	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	35	located	found	1446:1450	arg1	A					1461:1461	lipid A	1455:1461	lipid A from Rhodobacter sphaeroides	1455:1490	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	35	located	found	1446:1450	arg2	those					1440:1444	those	1440:1444	those	1440:1444	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	36	from	sphaeroides	1480:1490	arg1	A					1461:1461	lipid A	1455:1461	lipid A from Rhodobacter sphaeroides	1455:1490	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	1	37	theme	host	192:195	arg1	system					211:216	the host innate immune system	188:216	the host innate immune system	188:216	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	6	38	theme	[Glcα	887:891	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	38	theme	[Glcα	887:891	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	8	39	contain	contain	1201:1207	arg1	composition					1161:1171	The acyl composition	1152:1171	The acyl composition in lipid A	1152:1182	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	39	contain	contain	1201:1207	arg2	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	39	contain	contain	1201:1207	arg2	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	39	contain	contain	1201:1207	arg2	chains					1383:1388	two C10 fatty acyl chains	1364:1388	two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN)	1364:1419	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	39	contain	contain	1201:1207	arg2	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	5	40	theme	magnetic	686:693	arg1	resonance					695:703	nuclear magnetic resonance	678:703	nuclear magnetic resonance spectroscopy	678:716	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	8	41	theme	Rhodobacter	1468:1478	arg1	sphaeroides					1480:1490	Rhodobacter sphaeroides	1468:1490	Rhodobacter sphaeroides	1468:1490	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	1	42	theme	innate	197:202	arg1	system					211:216	the host innate immune system	188:216	the host innate immune system	188:216	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	3	43	theme	other	385:389	arg1	endotoxins					391:400	other endotoxins	385:400	other endotoxins	385:400	have been reported to produce LPS that lack endotoxic activity, and instead act as antagonists of other endotoxins.
30658251	5	44	theme	nuclear	678:684	arg1	resonance					695:703	nuclear magnetic resonance	678:703	nuclear magnetic resonance spectroscopy	678:716	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	0	45	from	Rhodobacter	62:72	arg1	lipooligosaccharide					31:49	a novel lipooligosaccharide	23:49	a novel lipooligosaccharide (LOS) from Rhodobacter	23:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	0	45	from	Rhodobacter	62:72	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter	0:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	0	45	from	Rhodobacter	62:72	arg1	LOS					52:54	LOS	52:54	LOS	52:54	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	4	46	with	correlation	575:585	arg1	production					597:606	IL-6 production	592:606	IL-6 production	592:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	8	47	dep	chains	1230:1235	arg1	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	47	dep	chains	1230:1235	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	47	dep	chains	1230:1235	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	1	48	theme	immune	204:209	arg1	system					211:216	the host innate immune system	188:216	the host innate immune system	188:216	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	0	49	theme	novel	25:29	arg1	lipooligosaccharide					31:49	a novel lipooligosaccharide	23:49	a novel lipooligosaccharide (LOS) from Rhodobacter	23:72	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	0	49	theme	novel	25:29	arg1	LOS					52:54	LOS	52:54	LOS	52:54	Structural analysis of a novel lipooligosaccharide (LOS) from Rhodobacter azotoformans.
30658251	6	50	theme	2 → 4	881:885	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	50	theme	2 → 4	881:885	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	3	51	theme	endotoxins	391:400	arg1	LPS					317:319	LPS	317:319	LPS that lack endotoxic activity, and instead act as antagonists of other endotoxins	317:400	have been reported to produce LPS that lack endotoxic activity, and instead act as antagonists of other endotoxins.
30658251	3	51	theme	endotoxins	391:400	arg1	antagonists					370:380	antagonists	370:380	antagonists of other endotoxins	370:400	have been reported to produce LPS that lack endotoxic activity, and instead act as antagonists of other endotoxins.
30658251	5	52	contain	containing	782:791	arg2	acid					804:807	glucuronic acid	793:807	glucuronic acid but not heptoses	793:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	5	52	contain	containing	782:791	arg1	structure					772:780	a shorter glycan structure	755:780	a shorter glycan structure containing glucuronic acid but not heptoses	755:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	5	52	contain	containing	782:791	arg2	heptoses					817:824	heptoses	817:824	glucuronic acid but not heptoses	793:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	6	53	theme	2 → 6	905:909	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	53	theme	2 → 6	905:909	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	54	theme	GlcNβ	924:928	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	54	theme	GlcNβ	924:928	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	5	55	theme	shorter	757:763	arg1	structure					772:780	a shorter glycan structure	755:780	a shorter glycan structure containing glucuronic acid but not heptoses	755:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	7	56	theme	variation	1108:1116	arg1	existence					1084:1092	the existence	1080:1092	the existence of structural variation caused by extension with hexoses	1080:1149	The structure was consistent with one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses.
30658251	8	57	theme	GlcN-1	1271:1276	arg1	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	57	theme	GlcN-1	1271:1276	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	57	theme	GlcN-1	1271:1276	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	58	theme	fatty	1372:1376	arg1	chains					1383:1388	two C10 fatty acyl chains	1364:1388	two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN)	1364:1419	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	5	59	theme	glycan	765:770	arg1	structure					772:780	a shorter glycan structure	755:780	a shorter glycan structure containing glucuronic acid but not heptoses	755:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	4	60	theme	Rhodobacter	500:510	arg1	azotoformans					512:523	Rhodobacter azotoformans	500:523	Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production	500:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	8	61	theme	β-hydroxyl	1342:1351	arg1	group					1353:1357	its β-hydroxyl group	1338:1357	its β-hydroxyl group	1338:1357	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	6	62	theme	GlcAα	864:868	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	62	theme	GlcAα	864:868	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	63	theme	novel	829:833	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	63	theme	novel	829:833	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	8	64	theme	lipid	1455:1459	arg1	A					1461:1461	lipid A	1455:1461	lipid A from Rhodobacter sphaeroides	1455:1490	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	65	theme	acyl	1156:1159	arg1	composition					1161:1171	The acyl composition	1152:1171	The acyl composition in lipid A	1152:1182	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	66	theme	3-OH-14:0 at	1279:1290	arg1	GlcN-1					1271:1276	GlcN-1	1271:1276	GlcN-1	1271:1276	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	66	theme	3-OH-14:0 at	1279:1290	arg1	N2					1292:1293	3-OH-14:0 at N2	1279:1293	3-OH-14:0 at N2 of GlcN-2	1279:1303	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	67	theme	3-oxo-14:0 at	1251:1263	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	67	theme	3-oxo-14:0 at	1251:1263	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	68	theme	fatty	1219:1223	arg1	3-OH-14:0					1238:1246	3-OH-14:0	1238:1246	3-OH-14:0	1238:1246	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	68	theme	fatty	1219:1223	arg1	N2					1265:1266	3-oxo-14:0 at N2	1251:1266	3-oxo-14:0 at N2	1251:1266	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	68	theme	fatty	1219:1223	arg1	chains					1230:1235	three C14 fatty acyl chains	1209:1235	three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group)	1209:1358	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	5	69	theme	mass	656:659	arg1	spectrometry					661:672	mass spectrometry	656:672	mass spectrometry	656:672	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	8	70	dep	chains	1383:1388	arg1	O3					1404:1405	3-OH-10:0 at O3	1391:1405	3-OH-10:0 at O3 of both GlcN	1391:1418	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	71	theme	lipid	1176:1180	arg1	A					1182:1182	lipid A	1176:1182	lipid A	1176:1182	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	8	72	from	Δ7	1332:1333	arg1	group					1353:1357	its β-hydroxyl group	1338:1357	its β-hydroxyl group	1338:1357	The acyl composition in lipid A was suggested to contain three C14 fatty acyl chains (3-OH-14:0 or 3-oxo-14:0 at N2 of GlcN-1, 3-OH-14:0 at N2 of GlcN-2, that carried another 14:1 Δ7 on its β-hydroxyl group) and two C10 fatty acyl chains (3-OH-10:0 at O3 of both GlcN), which are same as those found in lipid A from Rhodobacter sphaeroides.
30658251	6	73	theme	[4-phosphate	911:922	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	73	theme	[4-phosphate	911:922	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	7	74	theme	structural	1097:1106	arg1	variation					1108:1116	structural variation	1097:1116	structural variation caused by extension with hexoses	1097:1149	The structure was consistent with one obtained based on MS. The MS analysis further revealed the existence of structural variation caused by extension with hexoses.
30658251	4	75	contain	has	560:562	arg2	correlation					575:585	an inverse correlation	564:585	an inverse correlation with IL-6 production	564:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	4	75	contain	has	560:562	arg1	azotoformans					512:523	Rhodobacter azotoformans	500:523	Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production	500:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	4	76	theme	IL-6	592:595	arg1	production					597:606	IL-6 production	592:606	IL-6 production	592:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	1	77	theme	Toll-like	230:238	arg1	TLR					250:252	TLR	250:252	TLR	250:252	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	1	77	theme	Toll-like	230:238	arg1	receptor					240:247	Toll-like receptor	230:247	the Toll-like receptor (TLR) 4-MD-2 complex	226:268	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	5	78	theme	novel	731:735	arg1	LOS					737:739	a novel LOS	729:739	a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses	729:824	We analyzed their molecular structure by using mass spectrometry and nuclear magnetic resonance spectroscopy and report a novel LOS consisting of a shorter glycan structure containing glucuronic acid but not heptoses.
30658251	6	79	theme	1 → 4	870:874	arg1	GlcNα1-phosphate					937:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate	853:952	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	6	79	theme	1 → 4	870:874	arg1	structure					842:850	A novel glycan structure	827:850	A novel glycan structure	827:850	A novel glycan structure, Glcα(1 → 4)GlcAα(1 → 4)KDOα(2 → 4)[Glcα(1 → 5)]KDOα(2 → 6)[4-phosphate]GlcNβ(1 → 6) GlcNα1-phosphate, was proposed using NMR methods.
30658251	4	80	theme	inverse	567:573	arg1	correlation					575:585	an inverse correlation	564:585	an inverse correlation with IL-6 production	564:606	In this report, we focused on LPS, especially the lipooligosaccharide (LOS) fraction produced by Rhodobacter azotoformans that shows production of IL-8, but has an inverse correlation with IL-6 production.
30658251	1	81	theme	Gram-negative	136:148	arg1	surface					165:171	the Gram-negative bacterial cell surface	132:171	the Gram-negative bacterial cell surface	132:171	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30658251	1	82	theme	receptor	240:247	arg1	complex					262:268	the Toll-like receptor (TLR) 4-MD-2 complex	226:268	the Toll-like receptor (TLR) 4-MD-2 complex	226:268	Lipopolysaccharides (LPS) are components of the Gram-negative bacterial cell surface that stimulate the host innate immune system through the Toll-like receptor (TLR) 4-MD-2 complex.
30879651	5	0	dep	%	785:785	arg1	xylan					797:801	xylan	797:801	xylan	797:801	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	1	1	theme	great	182:186	arg1	importance					188:197	great importance	182:197	great importance	182:197	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	0	2	theme	films	88:92	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	0	2	theme	films	88:92	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	0	2	theme	films	88:92	arg1	roughing					50:57	enzymatic surface roughing	32:57	enzymatic surface roughing	32:57	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	6	3	from	nanoscale	1078:1086	arg1	etching/roughing					1058:1073	the subsequent surface etching/roughing	1035:1073	the subsequent surface etching/roughing at nanoscale using facile xylanase treatment	1035:1118	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	5	4	theme	2	852:852	arg1	loading					841:847	an enzyme loading	831:847	an enzyme loading of 2	831:852	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	6	5	from	located	946:952	arg1	surface					961:967	the surface	957:967	the surface of the composite films	957:990	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	2	6	theme	facile	348:353	arg1	method					372:377	facile solution-casting method	348:377	facile solution-casting method	348:377	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	4	7	theme	composite	653:661	arg1	films					663:667	the composite films	649:667	the composite films	649:667	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	5	8	theme	Xylanase	741:748	arg1	treatment					750:758	Xylanase treatment	741:758	Xylanase treatment	741:758	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	6	9	theme	subsequent	1039:1048	arg1	etching/roughing					1058:1073	the subsequent surface etching/roughing	1035:1073	the subsequent surface etching/roughing at nanoscale using facile xylanase treatment	1035:1118	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	2	10	theme	films	336:340	arg1	feasibility					271:281	the technical feasibility	257:281	the technical feasibility of fabricating transparent cellulose/xylan composite films	257:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	5	11	theme	composite	810:818	arg1	films					820:824	the composite films	806:824	the composite films with an enzyme loading of 2	806:852	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	2	12	theme	composite	326:334	arg1	films					336:340	fabricating transparent cellulose/xylan composite films	286:340	fabricating transparent cellulose/xylan composite films	286:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	4	13	theme	structural	718:727	arg1	advantages					729:738	their original mechanical and structural advantages	688:738	their original mechanical and structural advantages	688:738	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	5	14	theme	5 mg g-1	858:865	arg1	cellulose					867:875	5 mg g-1 cellulose	858:875	5 mg g-1 cellulose	858:875	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	5	15	theme	enzyme	834:839	arg1	loading					841:847	an enzyme loading	831:847	an enzyme loading of 2	831:852	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	5	16	theme	films	820:824	arg1	cellulose					867:875	5 mg g-1 cellulose	858:875	5 mg g-1 cellulose	858:875	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	5	16	theme	films	820:824	arg1	films					820:824	the composite films	806:824	the composite films with an enzyme loading of 2	806:852	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	5	16	theme	films	820:824	arg1	%					785:785	26.0%	781:785	26.0%	781:785	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	5	16	theme	films	820:824	arg1	%					795:795	32.3%	791:795	32.3%	791:795	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	1	17	theme	Upgrading	95:103	arg1	biopolymer					125:134	Upgrading renewable cellulose biopolymer	95:134	Upgrading renewable cellulose biopolymer to various high-value material/chemical	95:174	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	3	18	theme	xylanase	459:466	arg1	treatment					468:476	xylanase treatment	459:476	xylanase treatment	459:476	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	6	19	theme	films	986:990	arg1	surface					961:967	the surface	957:967	the surface of the composite films	957:990	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	1	20	theme	renewable	105:113	arg1	biopolymer					125:134	Upgrading renewable cellulose biopolymer	95:134	Upgrading renewable cellulose biopolymer to various high-value material/chemical	95:174	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	1	21	theme	sustainable	213:223	arg1	bio-economy					225:235	a sustainable bio-economy	211:235	a sustainable bio-economy	211:235	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	3	22	theme	technical	436:444	arg1	potential					446:454	the technical potential	432:454	the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications	432:588	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	5	23	with	films	820:824	arg1	loading					841:847	an enzyme loading	831:847	an enzyme loading of 2	831:852	Xylanase treatment specifically removed 26.0% and 32.3% xylan of the composite films with an enzyme loading of 2 and 5 mg g-1 cellulose, respectively.
30879651	1	24	theme	cellulose	115:123	arg1	biopolymer					125:134	Upgrading renewable cellulose biopolymer	95:134	Upgrading renewable cellulose biopolymer to various high-value material/chemical	95:174	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	3	25	theme	films	538:542	arg1	surface					504:510	the surface	500:510	the surface of the obtained composite films	500:542	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	2	26	theme	solution-casting	355:370	arg1	method					372:377	facile solution-casting method	348:377	facile solution-casting method	348:377	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	0	27	theme	surface	42:48	arg1	roughing					50:57	enzymatic surface roughing	32:57	enzymatic surface roughing	32:57	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	6	28	located	located	946:952	arg2	component					916:924	xylan component	910:924	xylan component	910:924	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	6	28	located	located	946:952	arg1	surface					961:967	the surface	957:967	the surface of the composite films	957:990	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	3	29	theme	obtained	519:526	arg1	films					538:542	the obtained composite films	515:542	the obtained composite films	515:542	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	0	30	theme	enzymatic	32:40	arg1	roughing					50:57	enzymatic surface roughing	32:57	enzymatic surface roughing	32:57	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	4	31	theme	Kraft	605:609	arg1	addition					617:624	bleached Kraft xylan addition	596:624	bleached Kraft xylan addition	596:624	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	6	32	theme	xylan	910:914	arg1	component					916:924	xylan component	910:924	xylan component	910:924	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	2	33	theme	technical	261:269	arg1	feasibility					271:281	the technical feasibility	257:281	the technical feasibility of fabricating transparent cellulose/xylan composite films	257:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	3	34	theme	composite	528:536	arg1	films					538:542	the obtained composite films	515:542	the obtained composite films	515:542	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	3	35	theme	treatment	468:476	arg1	potential					446:454	the technical potential	432:454	the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications	432:588	More importantly, this work also initially assessed the technical potential of xylanase treatment to selectively modify the surface of the obtained composite films with the goal of extending their applications.
30879651	4	36	theme	bleached	596:603	arg1	addition					617:624	bleached Kraft xylan addition	596:624	bleached Kraft xylan addition	596:624	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	6	37	from	surface	961:967	arg1	located					946:952	located	946:952	located	946:952	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	6	38	theme	structural	1147:1156	arg1	advantages					1158:1167	their structural advantages	1141:1167	their structural advantages	1141:1167	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	1	39	theme	various	139:145	arg1	material/chemical					158:174	various high-value material/chemical	139:174	various high-value material/chemical	139:174	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	4	40	theme	xylan	611:615	arg1	addition					617:624	bleached Kraft xylan addition	596:624	bleached Kraft xylan addition	596:624	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	2	41	theme	cellulose/xylan	310:324	arg1	films					336:340	fabricating transparent cellulose/xylan composite films	286:340	fabricating transparent cellulose/xylan composite films	286:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	1	42	theme	high-value	147:156	arg1	material/chemical					158:174	various high-value material/chemical	139:174	various high-value material/chemical	139:174	Upgrading renewable cellulose biopolymer to various high-value material/chemical is of great importance in building a sustainable bio-economy.
30879651	6	43	theme	surface	1050:1056	arg1	etching/roughing					1058:1073	the subsequent surface etching/roughing	1035:1073	the subsequent surface etching/roughing at nanoscale using facile xylanase treatment	1035:1118	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	2	44	theme	transparent	298:308	arg1	films					336:340	fabricating transparent cellulose/xylan composite films	286:340	fabricating transparent cellulose/xylan composite films	286:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	6	45	theme	facile	1094:1099	arg1	treatment					1110:1118	facile xylanase treatment	1094:1118	facile xylanase treatment	1094:1118	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	0	46	theme	composite	78:86	arg1	films					88:92	cellulose/xylan composite films	62:92	cellulose/xylan composite films	62:92	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	4	47	theme	mechanical	703:712	arg1	advantages					729:738	their original mechanical and structural advantages	688:738	their original mechanical and structural advantages	688:738	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	2	48	theme	fabricating	286:296	arg1	films					336:340	fabricating transparent cellulose/xylan composite films	286:340	fabricating transparent cellulose/xylan composite films	286:340	This work assessed the technical feasibility of fabricating transparent cellulose/xylan composite films using facile solution-casting method.
30879651	4	49	theme	original	694:701	arg1	advantages					729:738	their original mechanical and structural advantages	688:738	their original mechanical and structural advantages	688:738	When bleached Kraft xylan addition was lower than 20 wt%, the composite films could still retain their original mechanical and structural advantages.
30879651	0	50	theme	cellulose/xylan	62:76	arg1	films					88:92	cellulose/xylan composite films	62:92	cellulose/xylan composite films	62:92	Synthesis, characterization and enzymatic surface roughing of cellulose/xylan composite films.
30879651	6	51	theme	composite	976:984	arg1	films					986:990	the composite films	972:990	the composite films	972:990	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	6	52	theme	xylanase	1101:1108	arg1	treatment					1110:1118	facile xylanase treatment	1094:1118	facile xylanase treatment	1094:1118	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30879651	6	53	theme	film-casting	999:1010	arg1	process					1012:1018	film-casting process	999:1018	film-casting process	999:1018	It was shown that xylan component was heterogeneously located in the surface of the composite films during film-casting process, which allowed the subsequent surface etching/roughing at nanoscale using facile xylanase treatment without compromising their structural advantages.
30953722	1	0	theme	fundamental	137:147	arg1	bioactivities					149:161	fundamental bioactivities	137:161	fundamental bioactivities	137:161	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	1	1	theme	epithelial	219:228	arg1	transport					230:238	epithelial transport	219:238	epithelial transport	219:238	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	6	2	from	time	869:872	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	2	from	time	869:872	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	2	from	time	869:872	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	2	from	time	869:872	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	8	3	theme	new	1171:1173	arg1	kind					1175:1178	a new kind	1169:1178	a new kind of iron delivery for further study	1169:1213	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	3	4	from	mannose	465:471	arg1	ratio					539:543	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	0	5	theme	complex	81:87	arg1	studies					36:42	in vitro absorption studies	16:42	in vitro absorption studies	16:42	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	0	5	theme	complex	81:87	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	1	6	from	bioactivities	149:161	arg1	essential					124:132	essential	124:132	essential	124:132	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	3	7	theme	mannose	465:471	arg1	composition					450:460	a monosaccharide composition	433:460	a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	433:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	6	8	theme	FeSO4	855:859	arg1	concentration					825:837	same concentration	820:837	same concentration	820:837	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	8	theme	FeSO4	855:859	arg1	1.8-fold					843:850	1.8-fold	843:850	1.8-fold	843:850	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	3	9	from	ratio	539:543	arg1	composition					450:460	a monosaccharide composition	433:460	a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	433:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	6	10	theme	FVP-Fe	783:788	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	10	theme	FVP-Fe	783:788	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	10	theme	FVP-Fe	783:788	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	10	theme	FVP-Fe	783:788	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	1	11	from	essential	124:132	arg1	bioactivities					149:161	fundamental bioactivities	137:161	fundamental bioactivities	137:161	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	3	12	theme	15.13 kDa	418:426	arg1	weight					408:413	a molecular weight	396:413	a molecular weight of 15.13 kDa	396:426	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	4	13	theme	better	630:635	arg1	solubility					637:646	better solubility	630:646	better solubility	630:646	In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO4.
30953722	0	14	theme	Flammulina	94:103	arg1	velutipes					105:113	Flammulina velutipes	94:113	Flammulina velutipes	94:113	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	2	15	with	velutipes	339:347	arg1	structure					365:373	a specific structure	354:373	a specific structure	354:373	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	7	16	theme	transport	944:952	arg1	pathway					954:960	the peptide transporter (pepT1) active transport pathway	905:960	the peptide transporter (pepT1) active transport pathway	905:960	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	8	17	theme	F.	1137:1138	arg1	velutipes					1140:1148	F. velutipes	1137:1148	F. velutipes	1137:1148	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	2	18	theme	Flammulina	328:337	arg1	velutipes					339:347	Flammulina velutipes	328:347	Flammulina velutipes with a specific structure	328:373	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	7	19	theme	sample	984:989	arg1	efflux					970:975	the efflux	966:975	the efflux of the sample	966:989	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	6	20	theme	same	864:867	arg1	time					869:872	same time	864:872	same time	864:872	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	7	21	theme	proteins	1035:1042	arg1	transporter					1050:1060	multidrug-resistance proteins (MRP) transporter	1014:1060	multidrug-resistance proteins (MRP) transporter	1014:1060	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	7	22	theme	multidrug-resistance	1014:1033	arg1	MRP					1045:1047	MRP	1045:1047	MRP	1045:1047	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	7	22	theme	multidrug-resistance	1014:1033	arg1	proteins					1035:1042	multidrug-resistance proteins	1014:1042	multidrug-resistance proteins (MRP) transporter	1014:1060	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	6	23	theme	transport	763:771	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	23	theme	transport	763:771	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	23	theme	transport	763:771	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	23	theme	transport	763:771	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	4	24	theme	digestion	586:594	arg1	model					596:600	the vitro digestion model	576:600	the vitro digestion model	576:600	In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO4.
30953722	7	25	theme	peptide	909:915	arg1	transporter					917:927	peptide transporter	909:927	the peptide transporter (pepT1) active transport pathway	905:960	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	7	25	theme	peptide	909:915	arg1	pepT1					930:934	pepT1	930:934	pepT1	930:934	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	7	26	theme	active	937:942	arg1	pathway					954:960	the peptide transporter (pepT1) active transport pathway	905:960	the peptide transporter (pepT1) active transport pathway	905:960	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	5	27	theme	better	740:745	arg1	absorption					747:756	better absorption	740:756	better absorption	740:756	In the cell assay, FVP-Fe(III) showed lower cytotoxicity and better absorption.
30953722	8	28	theme	further	1201:1207	arg1	study					1209:1213	further study	1201:1213	further study	1201:1213	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	4	29	theme	vitro	580:584	arg1	model					596:600	the vitro digestion model	576:600	the vitro digestion model	576:600	In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO4.
30953722	8	30	theme	study	1083:1087	arg1	results					1067:1073	The results	1063:1073	The results of this study	1063:1087	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	6	31	from	concentration	825:837	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	31	from	concentration	825:837	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	31	from	concentration	825:837	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	31	from	concentration	825:837	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	2	32	theme	polysaccharide‑iron	263:281	arg1	FVP-Fe					297:302	FVP-Fe(III)	297:307	FVP-Fe(III)	297:307	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	2	32	theme	polysaccharide‑iron	263:281	arg1	complex					288:294	a novel polysaccharide‑iron(III) complex	255:294	a novel polysaccharide‑iron(III) complex (FVP-Fe(III))	255:308	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	2	33	theme	specific	356:363	arg1	structure					365:373	a specific structure	354:373	a specific structure	354:373	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	0	34	theme	in	16:17	arg1	studies					36:42	in vitro absorption studies	16:42	in vitro absorption studies	16:42	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	2	35	theme	novel	257:261	arg1	FVP-Fe					297:302	FVP-Fe(III)	297:307	FVP-Fe(III)	297:307	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	2	35	theme	novel	257:261	arg1	complex					288:294	a novel polysaccharide‑iron(III) complex	255:294	a novel polysaccharide‑iron(III) complex (FVP-Fe(III))	255:308	In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure.
30953722	5	36	theme	lower	717:721	arg1	cytotoxicity					723:734	lower cytotoxicity	717:734	lower cytotoxicity	717:734	In the cell assay, FVP-Fe(III) showed lower cytotoxicity and better absorption.
30953722	6	37	theme	same	820:823	arg1	concentration					825:837	same concentration	820:837	same concentration	820:837	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	38	from	1.8-fold	843:850	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	38	from	1.8-fold	843:850	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	38	from	1.8-fold	843:850	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	38	from	1.8-fold	843:850	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	39	dep	1.5-fold	799:806	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	39	dep	1.5-fold	799:806	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	39	dep	1.5-fold	799:806	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	39	dep	1.5-fold	799:806	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	0	40	theme	absorption	25:34	arg1	studies					36:42	in vitro absorption studies	16:42	in vitro absorption studies	16:42	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	5	41	theme	cell	686:689	arg1	assay					691:695	the cell assay	682:695	the cell assay	682:695	In the cell assay, FVP-Fe(III) showed lower cytotoxicity and better absorption.
30953722	3	42	contain	had	392:394	arg1	FVP-Fe					380:385	The FVP-Fe(III)	376:390	The FVP-Fe(III)	376:390	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	3	42	contain	had	392:394	arg2	weight					408:413	a molecular weight	396:413	a molecular weight of 15.13 kDa	396:426	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	4	43	theme	iron	662:665	arg1	solubility					637:646	better solubility	630:646	better solubility	630:646	In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO4.
30953722	6	44	theme	FeSO4	811:815	arg1	III					790:792	III	790:792	III	790:792	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	44	theme	FeSO4	811:815	arg1	1.5-fold					799:806	1.5-fold	799:806	1.5-fold	799:806	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	44	theme	FeSO4	811:815	arg1	FVP-Fe					783:788	FVP-Fe	783:788	FVP-Fe	783:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	6	44	theme	FeSO4	811:815	arg1	amount					773:778	The transport amount	759:778	The transport amount of FVP-Fe	759:788	The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time.
30953722	8	45	theme	iron	1183:1186	arg1	delivery					1188:1195	iron delivery	1183:1195	iron delivery	1183:1195	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	7	46	theme	transporter	917:927	arg1	pathway					954:960	the peptide transporter (pepT1) active transport pathway	905:960	the peptide transporter (pepT1) active transport pathway	905:960	The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter.
30953722	3	47	theme	molar	533:537	arg1	ratio					539:543	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	1	48	from	efficiency	197:206	arg1	transport					230:238	epithelial transport	219:238	epithelial transport	219:238	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	3	49	theme	galacturonic	474:485	arg1	mannose					465:471	mannose	465:471	mannose	465:471	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	3	49	theme	galacturonic	474:485	arg1	acid					487:490	galacturonic acid	474:490	galacturonic acid	474:490	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	8	50	theme	delivery	1188:1195	arg1	kind					1175:1178	a new kind	1169:1178	a new kind of iron delivery for further study	1169:1213	The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
30953722	0	51	dep	in	16:17	arg1	vitro					19:23	vitro	19:23	vitro	19:23	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	3	52	theme	molecular	398:406	arg1	weight					408:413	a molecular weight	396:413	a molecular weight of 15.13 kDa	396:426	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	0	53	theme	polysaccharide‑iron	55:73	arg1	complex					81:87	a novel polysaccharide‑iron (III) complex	47:87	a novel polysaccharide‑iron (III) complex	47:87	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	1	54	theme	iron	211:214	arg1	efficiency					197:206	the efficiency	193:206	the efficiency of iron on epithelial transport	193:238	Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport.
30953722	0	55	theme	novel	49:53	arg1	complex					81:87	a novel polysaccharide‑iron (III) complex	47:87	a novel polysaccharide‑iron (III) complex	47:87	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	3	56	theme	monosaccharide	435:448	arg1	composition					450:460	a monosaccharide composition	433:460	a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	433:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	3	57	from	composition	450:460	arg1	ratio					539:543	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9	531:570	The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9.
30953722	0	58	from	velutipes	105:113	arg1	studies					36:42	in vitro absorption studies	16:42	in vitro absorption studies	16:42	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
30953722	0	58	from	velutipes	105:113	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.
31548982	0	0	theme	nanocellulose	97:109	arg1	reinforcements					111:124	aligned nanocellulose reinforcements	89:124	aligned nanocellulose reinforcements	89:124	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	4	1	used	used	492:495	arg2	We					489:490	We	489:490	We	489:490	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	8	2	theme	protein	1086:1092	arg1	engineering					1094:1104	protein engineering	1086:1104	protein engineering	1086:1104	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	0	3	theme	aligned	89:95	arg1	reinforcements					111:124	aligned nanocellulose reinforcements	89:124	aligned nanocellulose reinforcements	89:124	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	8	4	theme	key	1162:1164	arg1	role					1166:1169	the key role	1158:1169	the key role of controlled assembly at multiple length scales for realization	1158:1234	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	5	5	theme	triblock	676:683	arg1	protein					685:691	triblock protein	676:691	triblock protein	676:691	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	6	6	from	structures	861:870	arg1	switching					835:843	subsequent conformational switching	809:843	subsequent conformational switching from disordered structures into β sheets	809:884	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	3	7	theme	major	393:397	arg1	challenge					399:407	A major challenge	391:407	A major challenge	391:407	A major challenge concerns balancing structure and functional properties in the assembly process.
31548982	4	8	theme	spider	578:583	arg1	silk					585:588	structurally modified spider silk	556:588	structurally modified spider silk	556:588	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	8	9	theme	controlled	1174:1183	arg1	assembly					1185:1192	controlled assembly	1174:1192	controlled assembly at multiple length scales	1174:1218	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	5	10	theme	protein	685:691	arg1	alignment					637:645	Flow alignment	632:645	Flow alignment of cellulose nanofibrils and triblock protein	632:691	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	8	11	theme	biological	1123:1132	arg1	materials					1134:1142	new fully biological materials	1113:1142	new fully biological materials	1113:1142	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	7	12	theme	high	976:979	arg1	strength					981:988	high strength	976:988	high strength	976:988	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	4	13	theme	triblock	523:530	arg1	architecture					532:543	triblock architecture	523:543	triblock architecture	523:543	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	5	14	theme	fiber	712:716	arg1	production					718:727	continuous fiber production	701:727	continuous fiber production	701:727	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	5	15	theme	continuous	701:710	arg1	production					718:727	continuous fiber production	701:727	continuous fiber production	701:727	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	6	16	theme	conformational	820:833	arg1	switching					835:843	subsequent conformational switching	809:843	subsequent conformational switching from disordered structures into β sheets	809:884	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	1	17	theme	future	192:197	arg1	materials					211:219	future sustainable materials	192:219	future sustainable materials	192:219	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	1	17	theme	future	192:197	arg1	potential					179:187	strong potential	172:187	strong potential	172:187	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	8	18	theme	assembly	1185:1192	arg1	role					1166:1169	the key role	1158:1169	the key role of controlled assembly at multiple length scales for realization	1158:1234	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	6	19	theme	subsequent	809:818	arg1	switching					835:843	subsequent conformational switching	809:843	subsequent conformational switching from disordered structures into β sheets	809:884	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	1	20	theme	sustainable	199:209	arg1	materials					211:219	future sustainable materials	192:219	future sustainable materials	192:219	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	1	20	theme	sustainable	199:209	arg1	potential					179:187	strong potential	172:187	strong potential	172:187	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	8	21	theme	length	1206:1211	arg1	scales					1213:1218	multiple length scales	1197:1218	multiple length scales	1197:1218	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	5	22	theme	cellulose	650:658	arg1	nanofibrils					660:670	cellulose nanofibrils	650:670	cellulose nanofibrils	650:670	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	3	23	theme	assembly	471:478	arg1	process					480:486	the assembly process	467:486	the assembly process	467:486	A major challenge concerns balancing structure and functional properties in the assembly process.
31548982	0	24	theme	Biomimetic	0:9	arg1	composites					11:20	Biomimetic composites	0:20	Biomimetic composites with enhanced toughening	0:45	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	8	25	theme	multiple	1197:1204	arg1	scales					1213:1218	multiple length scales	1197:1218	multiple length scales	1197:1218	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	5	26	theme	nanofibrils	660:670	arg1	alignment					637:645	Flow alignment	632:645	Flow alignment of cellulose nanofibrils and triblock protein	632:691	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	7	27	with	material	962:969	arg1	stiffness					994:1002	stiffness	994:1002	stiffness	994:1002	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	7	27	with	material	962:969	arg1	strength					981:988	high strength	976:988	high strength	976:988	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	7	28	theme	new	948:950	arg1	material					962:969	a new composite material	946:969	a new composite material with high strength and stiffness combined with increased toughness	946:1036	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	0	29	theme	enhanced	27:34	arg1	toughening					36:45	enhanced toughening	27:45	enhanced toughening	27:45	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	2	30	theme	reinforcing	342:352	arg1	component					354:362	the reinforcing component	338:362	the reinforcing component	338:362	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	7	31	theme	composite	952:960	arg1	material					962:969	a new composite material	946:969	a new composite material with high strength and stiffness combined with increased toughness	946:1036	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	8	32	from	role	1166:1169	arg1	scales					1213:1218	multiple length scales	1197:1218	multiple length scales	1197:1218	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	3	33	theme	balancing	418:426	arg1	structure					428:436	balancing structure	418:436	balancing structure	418:436	A major challenge concerns balancing structure and functional properties in the assembly process.
31548982	7	34	theme	a	916:916	arg1	adhesiveness					924:935	the matrix a tough adhesiveness	905:935	the matrix a tough adhesiveness	905:935	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	5	35	theme	Flow	632:635	arg1	alignment					637:645	Flow alignment	632:645	Flow alignment of cellulose nanofibrils and triblock protein	632:691	Flow alignment of cellulose nanofibrils and triblock protein allowed continuous fiber production.
31548982	4	36	theme	recombinant	497:507	arg1	proteins					509:516	recombinant proteins	497:516	recombinant proteins	497:516	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	6	37	theme	concentrated	778:789	arg1	coacervates					791:801	concentrated coacervates	778:801	concentrated coacervates	778:801	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	8	38	theme	new	1113:1115	arg1	materials					1134:1142	new fully biological materials	1113:1142	new fully biological materials	1113:1142	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	7	39	theme	tough	918:922	arg1	adhesiveness					924:935	the matrix a tough adhesiveness	905:935	the matrix a tough adhesiveness	905:935	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	2	40	contain	have	232:235	arg2	properties					251:260	complementary properties	237:260	complementary properties	237:260	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	2	40	contain	have	232:235	arg1	They					222:225	They	222:225	They	222:225	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	4	41	theme	terminal	595:602	arg1	modules					623:629	terminal cellulose affinity modules	595:629	terminal cellulose affinity modules	595:629	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	2	42	theme	suitable	263:270	arg1	properties					251:260	complementary properties	237:260	complementary properties	237:260	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	8	43	from	possibilities	1069:1081	arg1	engineering					1094:1104	protein engineering	1086:1104	protein engineering	1086:1104	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	0	44	theme	triblock	67:74	arg1	proteins					76:83	silk-inspired triblock proteins	53:83	silk-inspired triblock proteins	53:83	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	7	45	theme	matrix	909:914	arg1	adhesiveness					924:935	the matrix a tough adhesiveness	905:935	the matrix a tough adhesiveness	905:935	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	0	46	theme	silk-inspired	53:65	arg1	proteins					76:83	silk-inspired triblock proteins	53:83	silk-inspired triblock proteins	53:83	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	4	47	theme	affinity	614:621	arg1	modules					623:629	terminal cellulose affinity modules	595:629	terminal cellulose affinity modules	595:629	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	2	48	theme	complementary	237:249	arg1	properties					251:260	complementary properties	237:260	complementary properties	237:260	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	8	49	theme	design	1062:1067	arg1	possibilities					1069:1081	versatile design possibilities	1052:1081	versatile design possibilities in protein engineering	1052:1104	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	0	50	with	composites	11:20	arg1	toughening					36:45	enhanced toughening	27:45	enhanced toughening	27:45	Biomimetic composites with enhanced toughening using silk-inspired triblock proteins and aligned nanocellulose reinforcements.
31548982	2	51	theme	tough	377:381	arg1	matrix					383:388	the tough matrix	373:388	the tough matrix	373:388	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	4	52	theme	cellulose	604:612	arg1	modules					623:629	terminal cellulose affinity modules	595:629	terminal cellulose affinity modules	595:629	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	6	53	theme	β	877:877	arg1	sheets					879:884	β sheets	877:884	β sheets	877:884	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	6	54	theme	phase	756:760	arg1	separation					762:771	phase separation	756:771	phase separation into concentrated coacervates	756:801	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	8	55	theme	versatile	1052:1060	arg1	possibilities					1069:1081	versatile design possibilities	1052:1081	versatile design possibilities in protein engineering	1052:1104	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	2	56	theme	composite	291:299	arg1	materials					301:309	composite materials	291:309	composite materials where cellulose would form the reinforcing component and silk the tough matrix	291:388	They also have complementary properties, suitable for combination in composite materials where cellulose would form the reinforcing component and silk the tough matrix.
31548982	7	57	theme	increased	1018:1026	arg1	toughness					1028:1036	increased toughness	1018:1036	increased toughness	1018:1036	This process gave the matrix a tough adhesiveness, forming a new composite material with high strength and stiffness combined with increased toughness.
31548982	3	58	theme	functional	442:451	arg1	properties					453:462	functional properties	442:462	functional properties	442:462	A major challenge concerns balancing structure and functional properties in the assembly process.
31548982	4	59	theme	modified	569:576	arg1	silk					585:588	structurally modified spider silk	556:588	structurally modified spider silk	556:588	We used recombinant proteins with triblock architecture, combining structurally modified spider silk with terminal cellulose affinity modules.
31548982	6	60	theme	disordered	850:859	arg1	structures					861:870	disordered structures	850:870	disordered structures	850:870	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	1	61	theme	strong	172:177	arg1	materials					211:219	future sustainable materials	192:219	future sustainable materials	192:219	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	1	61	theme	strong	172:177	arg1	potential					179:187	strong potential	172:187	strong potential	172:187	Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
31548982	6	62	theme	Protein	730:736	arg1	assembly					738:745	Protein assembly	730:745	Protein assembly	730:745	Protein assembly involved phase separation into concentrated coacervates, with subsequent conformational switching from disordered structures into β sheets.
31548982	8	63	from	scales	1213:1218	arg1	role					1166:1169	the key role	1158:1169	the key role of controlled assembly at multiple length scales for realization	1158:1234	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31548982	8	63	from	scales	1213:1218	arg1	assembly					1185:1192	controlled assembly	1174:1192	controlled assembly at multiple length scales	1174:1218	We show that versatile design possibilities in protein engineering enable new fully biological materials and emphasize the key role of controlled assembly at multiple length scales for realization.
31930118	2	0	theme	total	347:351	arg1	TPC					373:375	TPC	373:375	TPC	373:375	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	2	0	theme	total	347:351	arg1	content					364:370	total polyphenol content	347:370	total polyphenol content (TPC) as phloroglucinol equivalents (PGE)	347:412	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	1	1	theme	Egregia	242:248	arg1	menziesii					250:258	Egregia menziesii	242:258	Egregia menziesii	242:258	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	11	2	theme	potential	2113:2121	arg1	candidates					2123:2132	potential candidates	2113:2132	potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management	2113:2242	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	10	3	theme	sulfated	1991:1998	arg1	polysaccharides					2000:2014	sulfated polysaccharides	1991:2014	sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate)	1991:2056	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	4	4	theme	abundant	709:716	arg1	phlorotannins					718:730	the most abundant phlorotannins	700:730	the most abundant phlorotannins	700:730	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	1	5	from	coast	296:300	arg1	seaweeds					141:148	five brown seaweeds	130:148	five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast	130:300	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	3	6	dep	activity	565:572	arg1	ORAC					584:587	ORAC	584:587	ORAC	584:587	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	3	6	dep	activity	565:572	arg1	DPPH					575:578	DPPH	575:578	DPPH	575:578	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	7	7	theme	inhibitory	1493:1502	arg1	capacities					1504:1513	α-glucosidase inhibitory capacities	1479:1513	α-glucosidase inhibitory capacities	1479:1513	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	6	8	theme	dry	1063:1065	arg1	extracts					1067:1074	arborea dry extracts	1055:1074	arborea dry extracts	1055:1074	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	3	9	theme	radical	546:552	arg1	activity					565:572	their radical scavenging activity	540:572	their radical scavenging activity (DPPH and ORAC)	540:588	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	7	10	theme	IC50	1363:1366	arg1	DPPH					1368:1371	IC50 DPPH 1.7 and 3.7 mg mL-1	1363:1391	DPPH	1368:1371	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	1	11	theme	products	93:100	arg1	search					59:64	search	59:64	search of pharmaceutically active products to control type 2 diabetes	59:127	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	8	12	theme	abundant	1617:1624	arg1	phlorotannins					1626:1638	The most abundant phlorotannins	1608:1638	The most abundant phlorotannins identified in the extracts	1608:1665	The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins.
31930118	8	12	theme	abundant	1617:1624	arg1	phloretol					1672:1680	phloretol	1672:1680	phloretol	1672:1680	The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins.
31930118	6	13	theme	soluble	1240:1246	arg1	polysaccharide					1248:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide	1152:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	13	theme	soluble	1240:1246	arg1	%					1275:1275	19.7 and 53%	1264:1275	19.7 and 53%	1264:1275	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	14	theme	arborea	1055:1061	arg1	extracts					1067:1074	arborea dry extracts	1055:1074	arborea dry extracts	1055:1074	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	7	15	theme	highest	1333:1339	arg1	activity					1353:1360	the highest antioxidant activity	1329:1360	the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract)	1329:1447	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	5	16	contain	contained	802:810	arg1	extracts					793:800	Hydroethanolic extracts	778:800	Hydroethanolic extracts	778:800	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	%					980:980	5 to 12%	973:980	5 to 12%	973:980	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	lipids					965:970	lipids	965:970	lipids (5 to 12%)	965:981	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	polysaccharides					890:904	ethanol-insoluble polysaccharides	872:904	ethanol-insoluble polysaccharides (5.4 to 53%)	872:917	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	TPC					988:990	TPC	988:990	TPC	988:990	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	%					868:868	4 to 9%	862:868	4 to 9%	862:868	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	extract					934:940	nitrogen-free extract	920:940	nitrogen-free extract	920:940	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	PGE					1007:1009	2.6 to 47.7 g PGE	993:1009	2.6 to 47.7 g PGE	993:1009	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	minerals					812:819	minerals	812:819	minerals (17 to 59% of the dry matter)	812:849	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	%					961:961	24.4 to 70.1%	949:961	24.4 to 70.1%	949:961	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	NFE					943:945	NFE	943:945	NFE	943:945	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	16	contain	contained	802:810	arg2	proteins					852:859	proteins	852:859	proteins (4 to 9%)	852:869	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	7	17	dep	ORAC	1394:1397	arg1	extract					1440:1446	equivalent/g extract	1427:1446	equivalent/g extract	1427:1446	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	5	18	dep	53	914:915	arg1	to					911:912	to	911:912	to	911:912	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	7	19	theme	arborea	1308:1314	arg1	extracts					1316:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	11	20	theme	pharmaceutical	2174:2187	arg1	preparations					2189:2200	nutraceutical and pharmaceutical preparations	2156:2200	nutraceutical and pharmaceutical preparations	2156:2200	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	4	21	theme	ATR-FT-IR	654:662	arg1	methodologies					664:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	10	22	with	related	1932:1938	arg1	contents					1951:1958	their contents	1945:1958	their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate)	1945:2056	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	1	23	theme	brown	135:139	arg1	seaweeds					141:148	five brown seaweeds	130:148	five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast	130:300	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	7	24	theme	equivalent/g	1427:1438	arg1	extract					1440:1446	equivalent/g extract	1427:1446	equivalent/g extract	1427:1446	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	2	25	theme	respective	469:478	arg1	extracts					503:510	their respective hydroethanolic (1 : 1) extracts	463:510	their respective hydroethanolic (1 : 1) extracts	463:510	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	10	26	theme	arborea	1903:1909	arg1	extracts					1911:1918	arborea extracts	1903:1918	arborea extracts	1903:1918	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	6	27	theme	lowest	1090:1095	arg1	content					1101:1107	the lowest ash content	1086:1107	the lowest ash content (26 and 17%, respectively)	1086:1134	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	7	28	theme	0.801 mmol	1409:1418	arg1	Trolox					1420:1425	0.801 mmol Trolox	1409:1425	0.801 mmol Trolox	1409:1425	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	4	29	from	polysaccharides	745:759	arg1	extracts					768:775	the extracts	764:775	the extracts	764:775	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	5	30	dep	47.7 g	1000:1005	arg1	to					997:998	to	997:998	to	997:998	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	2	31	theme	phloroglucinol	381:394	arg1	PGE					409:411	PGE	409:411	PGE	409:411	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	2	31	theme	phloroglucinol	381:394	arg1	equivalents					396:406	phloroglucinol equivalents	381:406	phloroglucinol equivalents (PGE)	381:412	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	8	32	theme	three-phloroglucinol	1713:1732	arg1	phlorotannins					1745:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins.
31930118	10	33	theme	PGU	1969:1971	arg1	phlorotannins					1973:1985	three PGU phlorotannins	1963:1985	three PGU phlorotannins	1963:1985	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	1	34	dep	seaweeds	141:148	arg1	Cystoseira					171:180	Cystoseira	171:180	Cystoseira	171:180	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	1	34	dep	seaweeds	141:148	arg1	osmundacea					182:191	osmundacea	182:191	osmundacea	182:191	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	0	35	theme	Nutraceutical	0:12	arg1	Potential					14:22	Nutraceutical Potential	0:22	Nutraceutical Potential of Five	0:30	Nutraceutical Potential of Five Mexican Brown Seaweeds.
31930118	5	36	theme	ethanol-insoluble	872:888	arg1	polysaccharides					890:904	ethanol-insoluble polysaccharides	872:904	ethanol-insoluble polysaccharides (5.4 to 53%)	872:917	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	36	theme	ethanol-insoluble	872:888	arg1	proteins					852:859	proteins	852:859	proteins (4 to 9%)	852:869	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	36	theme	ethanol-insoluble	872:888	arg1	%					916:916	5.4 to 53%	907:916	5.4 to 53%	907:916	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	10	37	dep	compressa	1886:1894	arg1	extracts					1911:1918	arborea extracts	1903:1918	arborea extracts	1903:1918	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	6	38	theme	100 g	1198:1202	arg1	extract					1204:1210	100 g extract	1198:1210	100 g extract	1198:1210	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	5	39	theme	100 g	1015:1019	arg1	extract					1025:1031	100 g dry extract	1015:1031	100 g dry extract	1015:1031	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	2	40	dep	1 	496:497	arg1	 1					499:500	 1	499:500	 1	499:500	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	1	41	theme	control	105:111	arg1	diabetes					120:127	control type 2 diabetes	105:127	control type 2 diabetes	105:127	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	0	42	dep	Seaweeds	46:53	arg1	Potential					14:22	Nutraceutical Potential	0:22	Nutraceutical Potential of Five	0:30	Nutraceutical Potential of Five Mexican Brown Seaweeds.
31930118	7	43	theme	194	1564:1566	arg1	mL-1					1579:1582	194 and 647 μg mL-1	1564:1582	mL-1	1579:1582	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	2	44	theme	seaweed	443:449	arg1	powders					451:457	the five seaweed powders	434:457	the five seaweed powders	434:457	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	3	45	theme	inhibitory	606:615	arg1	activity					617:624	glycosidase inhibitory activity	594:624	glycosidase inhibitory activity	594:624	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	7	46	theme	α-glucosidase	1479:1491	arg1	capacities					1504:1513	α-glucosidase inhibitory capacities	1479:1513	α-glucosidase inhibitory capacities	1479:1513	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	2	47	theme	Proximate	321:329	arg1	composition					331:341	Proximate composition	321:341	Proximate composition	321:341	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	2	48	dep	hydroethanolic	480:493	arg1	1 					496:497	1 	496:497	1 : 1	496:500	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	7	49	dep	IC50	1516:1519	arg1	mL-1					1537:1540	mL-1	1537:1540	mL-1	1537:1540	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	1	50	dep	osmundacea	182:191	arg1	californica					225:235	Pterygophora californica	212:235	Pterygophora californica	212:235	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	1	50	dep	osmundacea	182:191	arg1	menziesii					250:258	Egregia menziesii	242:258	Egregia menziesii	242:258	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	1	50	dep	osmundacea	182:191	arg1	arborea					203:209	Ecklonia arborea	194:209	Ecklonia arborea	194:209	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	5	51	theme	dry	839:841	arg1	matter					843:848	the dry matter	835:848	the dry matter	835:848	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	7	52	dep	α-amylase	1465:1473	arg1	the					1453:1455	the	1453:1455	the	1453:1455	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	52	dep	α-amylase	1465:1473	arg1	IC50					1516:1519	IC50	1516:1519	IC50 940 and 1152 μg mL-1	1516:1540	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	2	53	theme	polyphenol	353:362	arg1	TPC					373:375	TPC	373:375	TPC	373:375	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	2	53	theme	polyphenol	353:362	arg1	content					364:370	total polyphenol content	347:370	total polyphenol content (TPC) as phloroglucinol equivalents (PGE)	347:412	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	10	54	theme	polysaccharides	2000:2014	arg1	contents					1951:1958	their contents	1945:1958	their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate)	1945:2056	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	5	55	dep	%	961:961	arg1	to					954:955	to	954:955	to	954:955	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	6	56	theme	ash	1097:1099	arg1	content					1101:1107	the lowest ash content	1086:1107	the lowest ash content (26 and 17%, respectively)	1086:1134	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	57	theme	NFE	1214:1216	arg1	polysaccharide					1248:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide	1152:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	57	theme	NFE	1214:1216	arg1	%					1275:1275	19.7 and 53%	1264:1275	19.7 and 53%	1264:1275	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	5	58	dep	minerals	812:819	arg1	%					830:830	17 to 59%	822:830	17 to 59% of the dry matter	822:848	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	58	dep	minerals	812:819	arg1	matter					843:848	the dry matter	835:848	the dry matter	835:848	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	6	59	dep	content	1101:1107	arg1	%					1119:1119	26 and 17%	1110:1119	26 and 17%	1110:1119	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	60	contain	had	1140:1142	arg2	contents					1278:1285	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	60	contain	had	1140:1142	arg2	some					1144:1147	some	1144:1147	some	1144:1147	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	60	contain	had	1140:1142	arg1	compressa					1038:1046	compressa	1038:1046	compressa	1038:1046	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	4	61	theme	HPLC-DAD	627:634	arg1	methodologies					664:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	5	62	theme	matter	843:848	arg1	%					830:830	17 to 59%	822:830	17 to 59% of the dry matter	822:848	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	62	theme	matter	843:848	arg1	matter					843:848	the dry matter	835:848	the dry matter	835:848	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	4	63	theme	HPLC-MS-TOF	637:647	arg1	methodologies					664:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	6	64	theme	polysaccharide	1248:1261	arg1	contents					1278:1285	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	10	65	dep	fucoidan	2023:2030	arg1	e.g.					2017:2020	e.g.	2017:2020	e.g.	2017:2020	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	7	66	theme	antioxidant	1341:1351	arg1	activity					1353:1360	the highest antioxidant activity	1329:1360	the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract)	1329:1447	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	67	dep	mL-1	1537:1540	arg1	1152 μg					1529:1535	1152 μg	1529:1535	1152 μg	1529:1535	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	67	dep	mL-1	1537:1540	arg1	940					1521:1523	940	1521:1523	940	1521:1523	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	1	68	theme	Ecklonia	194:201	arg1	arborea					203:209	Ecklonia arborea	194:209	Ecklonia arborea	194:209	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	10	69	theme	E.	1900:1901	arg1	related					1932:1938	related	1932:1938	related	1932:1938	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	10	69	theme	E.	1900:1901	arg1	bioactivities					1866:1878	The bioactivities	1862:1878	The bioactivities of S. compressa and E. arborea extracts	1862:1918	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	3	70	theme	scavenging	554:563	arg1	activity					565:572	their radical scavenging activity	540:572	their radical scavenging activity (DPPH and ORAC)	540:588	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	1	71	theme	type	113:116	arg1	diabetes					120:127	control type 2 diabetes	105:127	control type 2 diabetes	105:127	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	10	72	theme	compressa	1886:1894	arg1	related					1932:1938	related	1932:1938	related	1932:1938	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	10	72	theme	compressa	1886:1894	arg1	bioactivities					1866:1878	The bioactivities	1862:1878	The bioactivities of S. compressa and E. arborea extracts	1862:1918	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	11	73	dep	suggest	2073:2079	arg1	candidates					2123:2132	potential candidates	2113:2132	potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management	2113:2242	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	7	74	theme	highest	1457:1463	arg1	α-amylase					1465:1473	α-amylase	1465:1473	α-amylase	1465:1473	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	75	theme	E.	1305:1306	arg1	extracts					1316:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	76	dep	DPPH	1368:1371	arg1	ORAC					1394:1397	ORAC 0.817	1394:1403	ORAC 0.817	1394:1403	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	76	dep	DPPH	1368:1371	arg1	Trolox					1420:1425	0.801 mmol Trolox	1409:1425	0.801 mmol Trolox	1409:1425	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	11	77	dep	compressa	2084:2092	arg1	arborea					2101:2107	arborea	2101:2107	arborea	2101:2107	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	7	78	theme	compressa	1291:1299	arg1	extracts					1316:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	2	79	theme	hydroethanolic	480:493	arg1	extracts					503:510	their respective hydroethanolic (1 : 1) extracts	463:510	their respective hydroethanolic (1 : 1) extracts	463:510	Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts.
31930118	6	80	dep	compressa	1038:1046	arg1	extracts					1067:1074	arborea dry extracts	1055:1074	arborea dry extracts	1055:1074	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	81	theme	phenolic	1164:1171	arg1	polysaccharide					1248:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide	1152:1261	the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents	1152:1285	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	81	theme	phenolic	1164:1171	arg1	%					1275:1275	19.7 and 53%	1264:1275	19.7 and 53%	1264:1275	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	7	82	dep	activity	1353:1360	arg1	DPPH					1368:1371	IC50 DPPH 1.7 and 3.7 mg mL-1	1363:1391	DPPH	1368:1371	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	82	dep	activity	1353:1360	arg1	3.7 mg mL-1					1381:1391	3.7 mg mL-1	1381:1391	3.7 mg mL-1	1381:1391	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	4	83	used	used	683:686	arg2	methodologies					664:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies	627:676	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	7	84	contain	had	1325:1327	arg2	α-amylase					1465:1473	α-amylase	1465:1473	α-amylase	1465:1473	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	84	contain	had	1325:1327	arg1	extracts					1316:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts	1288:1323	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	7	84	contain	had	1325:1327	arg2	activity					1353:1360	the highest antioxidant activity	1329:1360	the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract)	1329:1447	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	11	85	theme	diabetes	2224:2231	arg1	management					2233:2242	diabetes management	2224:2242	diabetes management	2224:2242	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	4	86	theme	sulfated	736:743	arg1	polysaccharides					745:759	sulfated polysaccharides	736:759	sulfated polysaccharides	736:759	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	8	87	theme	unit	1734:1737	arg1	phlorotannins					1745:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins.
31930118	6	88	dep	phenolic	1164:1171	arg1	PGE					1190:1192	47.7 and 15.2 g PGE	1174:1192	47.7 and 15.2 g PGE	1174:1192	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	6	88	dep	phenolic	1164:1171	arg1	highest					1156:1162	highest	1156:1162	highest	1156:1162	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	10	89	theme	phlorotannins	1973:1985	arg1	contents					1951:1958	their contents	1945:1958	their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate)	1945:2056	The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate).
31930118	5	90	dep	9	867:867	arg1	to					864:865	to	864:865	to	864:865	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	8	91	theme	PGU	1740:1742	arg1	phlorotannins					1745:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	three-phloroglucinol unit (PGU) phlorotannins	1713:1757	The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins.
31930118	4	92	from	phlorotannins	718:730	arg1	extracts					768:775	the extracts	764:775	the extracts	764:775	HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts.
31930118	1	93	theme	active	86:91	arg1	products					93:100	pharmaceutically active products	69:100	pharmaceutically active products	69:100	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	0	94	theme	Five	27:30	arg1	Potential					14:22	Nutraceutical Potential	0:22	Nutraceutical Potential of Five	0:30	Nutraceutical Potential of Five Mexican Brown Seaweeds.
31930118	11	95	theme	nutraceutical	2156:2168	arg1	preparations					2189:2200	nutraceutical and pharmaceutical preparations	2156:2200	nutraceutical and pharmaceutical preparations	2156:2200	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	5	96	theme	dry	1021:1023	arg1	extract					1025:1031	100 g dry extract	1015:1031	100 g dry extract	1015:1031	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	97	dep	12	978:979	arg1	to					975:976	to	975:976	to	975:976	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	3	98	theme	glycosidase	594:604	arg1	activity					617:624	glycosidase inhibitory activity	594:624	glycosidase inhibitory activity	594:624	Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity.
31930118	5	99	theme	Hydroethanolic	778:791	arg1	extracts					793:800	Hydroethanolic extracts	778:800	Hydroethanolic extracts	778:800	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	6	100	dep	NFE	1214:1216	arg1	%					1232:1232	57.3 and 70.1%	1219:1232	57.3 and 70.1%	1219:1232	S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents.
31930118	7	101	theme	647 μg	1572:1577	arg1	mL-1					1579:1582	194 and 647 μg mL-1	1564:1582	mL-1	1579:1582	S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL-1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL-1 against α-amylase and 194 and 647 μg mL-1 against α-glucosidase).
31930118	9	102	theme	sulfated	1809:1816	arg1	polysaccharides					1818:1832	the sulfated polysaccharides	1805:1832	the sulfated polysaccharides identified in the extracts	1805:1859	Laminarin, fucoidan, and alginate were among the sulfated polysaccharides identified in the extracts.
31930118	11	103	theme	food	2138:2141	arg1	products					2143:2150	food products	2138:2150	food products	2138:2150	These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
31930118	1	104	theme	Pterygophora	212:223	arg1	californica					225:235	Pterygophora californica	212:235	Pterygophora californica	212:235	In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated.
31930118	5	105	theme	nitrogen-free	920:932	arg1	extract					934:940	nitrogen-free extract	920:940	nitrogen-free extract	920:940	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	105	theme	nitrogen-free	920:932	arg1	proteins					852:859	proteins	852:859	proteins (4 to 9%)	852:869	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
31930118	5	106	dep	59	828:829	arg1	to					825:826	to	825:826	to	825:826	Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract).
30626118	3	0	dep	16.20	610:614	arg1	to					607:608	to	607:608	to	607:608	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	6	1	theme	hemicelluloses	989:1002	arg1	composition					1004:1014	hemicelluloses composition	989:1014	hemicelluloses composition	989:1014	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	6	2	theme	biochemicals	1089:1100	arg1	fields					1069:1074	the fields	1065:1074	the fields of biofuels, biochemicals, and biomaterials	1065:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	0	3	theme	Maize	135:139	arg1	Stems					141:145	Sweet Maize Stems	129:145	Sweet Maize Stems	129:145	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	6	4	from	information	974:984	arg1	fields					1069:1074	the fields	1065:1074	the fields of biofuels, biochemicals, and biomaterials	1065:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	6	4	from	information	974:984	arg1	structure					1020:1028	structure	1020:1028	structure	1020:1028	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	6	4	from	information	974:984	arg1	composition					1004:1014	hemicelluloses composition	989:1014	hemicelluloses composition	989:1014	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	4	5	theme	branched	631:638	arg1	hemicelluloses					640:653	More highly branched hemicelluloses	619:653	More highly branched hemicelluloses	619:653	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	1	6	theme	hot	184:186	arg1	water					188:192	hot water	184:192	hot water	184:192	Sweet maize stems were treated with hot water and potassium hydroxide to fractionate hemicellulosic polymers.
30626118	0	7	theme	Sweet	129:133	arg1	Stems					141:145	Sweet Maize Stems	129:145	Sweet Maize Stems	129:145	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	5	8	theme	alkali-soluble	848:861	arg1	hemicelluloses					863:876	alkali-soluble hemicelluloses	848:876	alkali-soluble hemicelluloses fractionated from sweet maize stems	848:912	Structural characterizations indicated that alkali-soluble hemicelluloses fractionated from sweet maize stems were mainly arabinoxylans.
30626118	3	9	theme	alkali-soluble	413:426	arg1	hemicelluloses					428:441	alkali-soluble hemicelluloses	413:441	alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%)	413:536	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	4	10	from	concentration	693:705	arg1	precipitated					668:679	precipitated	668:679	precipitated	668:679	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	4	11	theme	solution	718:725	arg1	concentration					693:705	a higher concentration	684:705	a higher concentration of ethanol solution	684:725	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	3	12	dep	solution	494:501	arg1	%					526:526	65%	524:526	65%	524:526	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	3	12	dep	solution	494:501	arg1	%					506:506	10%	504:506	10%	504:506	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	3	12	dep	solution	494:501	arg1	%					516:516	35%	514:516	35%	514:516	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	3	12	dep	solution	494:501	arg1	%					535:535	80%	533:535	80%	533:535	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	3	12	dep	solution	494:501	arg1	%					511:511	20%	509:511	20%	509:511	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	3	12	dep	solution	494:501	arg1	%					521:521	50%	519:521	50%	519:521	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	6	13	theme	fundamental	962:972	arg1	information					974:984	fundamental information	962:984	fundamental information on hemicelluloses composition and structure	962:1028	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	4	14	theme	ethanol	710:716	arg1	solution					718:725	ethanol solution	710:725	ethanol solution	710:725	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	1	15	theme	potassium	198:206	arg1	hydroxide					208:216	potassium hydroxide	198:216	potassium hydroxide	198:216	Sweet maize stems were treated with hot water and potassium hydroxide to fractionate hemicellulosic polymers.
30626118	0	16	theme	Water-	23:28	arg1	Hemicelluloses					49:62	the Water- and Alkali-Soluble Hemicelluloses	19:62	the Water- and Alkali-Soluble Hemicelluloses	19:62	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	3	17	dep	88.62	579:583	arg1	to					576:577	to	576:577	to	576:577	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	4	18	theme	arabinose	768:776	arg1	ratio					778:782	arabinose ratio	768:782	arabinose ratio from 16.43 to 4.21	768:801	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	4	19	from	16.43	789:793	arg1	ratio					778:782	arabinose ratio	768:782	arabinose ratio from 16.43 to 4.21	768:801	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	5	20	theme	Structural	804:813	arg1	characterizations					815:831	Structural characterizations	804:831	Structural characterizations	804:831	Structural characterizations indicated that alkali-soluble hemicelluloses fractionated from sweet maize stems were mainly arabinoxylans.
30626118	0	21	theme	Alkali-Soluble	34:47	arg1	Hemicelluloses					49:62	the Water- and Alkali-Soluble Hemicelluloses	19:62	the Water- and Alkali-Soluble Hemicelluloses	19:62	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	6	22	theme	potential	1040:1048	arg1	utilization					1050:1060	their potential utilization	1034:1060	their potential utilization in the fields of biofuels, biochemicals, and biomaterials	1034:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	5	23	theme	maize	902:906	arg1	stems					908:912	sweet maize stems	896:912	sweet maize stems	896:912	Structural characterizations indicated that alkali-soluble hemicelluloses fractionated from sweet maize stems were mainly arabinoxylans.
30626118	1	24	theme	hemicellulosic	233:246	arg1	polymers					248:255	hemicellulosic polymers	233:255	hemicellulosic polymers	233:255	Sweet maize stems were treated with hot water and potassium hydroxide to fractionate hemicellulosic polymers.
30626118	4	25	theme	decreasing	747:756	arg1	xylose					758:763	the decreasing xylose	743:763	the decreasing xylose to arabinose ratio from 16.43 to 4.21	743:801	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	4	26	theme	higher	686:691	arg1	concentration					693:705	a higher concentration	684:705	a higher concentration of ethanol solution	684:725	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	3	27	theme	graded	479:484	arg1	solution					494:501	a graded ethanol solution	477:501	a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%)	477:536	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	0	28	theme	Hemicelluloses	49:62	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of the Water- and Alkali-Soluble Hemicelluloses	0:62	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	6	29	from	utilization	1050:1060	arg1	fields					1069:1074	the fields	1065:1074	the fields of biofuels, biochemicals, and biomaterials	1065:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	6	29	from	utilization	1050:1060	arg1	structure					1020:1028	structure	1020:1028	structure	1020:1028	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	6	29	from	utilization	1050:1060	arg1	composition					1004:1014	hemicelluloses composition	989:1014	hemicelluloses composition	989:1014	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	3	30	theme	ethanol	486:492	arg1	solution					494:501	a graded ethanol solution	477:501	a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%)	477:536	In comparison, alkali-soluble hemicelluloses fractionated by acidification and a graded ethanol solution (10%, 20%, 35%, 50%, 65%, and 80%) were mainly composed of xylose (69.73 to 88.62%) and arabinose (5.41 to 16.20%).
30626118	2	31	theme	water-soluble	286:298	arg1	hemicelluloses					300:313	the water-soluble hemicelluloses	282:313	the water-soluble hemicelluloses	282:313	The results showed that the water-soluble hemicelluloses were mainly composed of glucose (27.83%), xylose (27.32%), and galactose (16.81%).
30626118	4	32	from	precipitated	668:679	arg1	concentration					693:705	a higher concentration	684:705	a higher concentration of ethanol solution	684:725	More highly branched hemicelluloses tended to be precipitated in a higher concentration of ethanol solution, as revealed by the decreasing xylose to arabinose ratio from 16.43 to 4.21.
30626118	1	33	theme	Sweet	148:152	arg1	stems					160:164	Sweet maize stems	148:164	Sweet maize stems	148:164	Sweet maize stems were treated with hot water and potassium hydroxide to fractionate hemicellulosic polymers.
30626118	6	34	theme	biofuels	1079:1086	arg1	fields					1069:1074	the fields	1065:1074	the fields of biofuels, biochemicals, and biomaterials	1065:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	1	35	theme	maize	154:158	arg1	stems					160:164	Sweet maize stems	148:164	Sweet maize stems	148:164	Sweet maize stems were treated with hot water and potassium hydroxide to fractionate hemicellulosic polymers.
30626118	0	36	theme	Sequential	80:89	arg1	Acidification					91:103	Sequential Acidification	80:103	Sequential Acidification	80:103	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	6	37	theme	biomaterials	1107:1118	arg1	fields					1069:1074	the fields	1065:1074	the fields of biofuels, biochemicals, and biomaterials	1065:1118	The results provided fundamental information on hemicelluloses composition and structure and their potential utilization in the fields of biofuels, biochemicals, and biomaterials.
30626118	5	38	theme	sweet	896:900	arg1	stems					908:912	sweet maize stems	896:912	sweet maize stems	896:912	Structural characterizations indicated that alkali-soluble hemicelluloses fractionated from sweet maize stems were mainly arabinoxylans.
30626118	0	39	from	Stems	141:145	arg1	Acidification					91:103	Sequential Acidification	80:103	Sequential Acidification	80:103	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
30626118	0	39	from	Stems	141:145	arg1	Graded-Ethanol					109:122	Graded-Ethanol	109:122	Graded-Ethanol	109:122	Characteristics of the Water- and Alkali-Soluble Hemicelluloses Fractionated by Sequential Acidification and Graded-Ethanol from Sweet Maize Stems.
31226371	0	0	theme	Ca2+	86:89	arg1	films					134:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	10	1	with	films	1255:1259	arg1	properties					1275:1284	improved properties	1266:1284	improved properties	1266:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	2	2	theme	X-ray	442:446	arg1	XRD					461:463	XRD	461:463	XRD	461:463	Structure was analyzed by using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
31226371	2	2	theme	X-ray	442:446	arg1	diffraction					448:458	X-ray diffraction	442:458	X-ray diffraction (XRD)	442:464	Structure was analyzed by using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
31226371	7	3	theme	blended	915:921	arg1	films					923:927	the blended films	911:927	the blended films	911:927	Moreover, KGM improved the release capacity of the blended films, while enhancing antimicrobial activity against Escherichia coli and Staphylococcus aureus.
31226371	6	4	theme	KGM	803:805	arg1	addition					791:798	The addition	787:798	The addition of KGM	787:805	The addition of KGM improved the mechanical strength of films significantly.
31226371	6	5	theme	mechanical	820:829	arg1	strength					831:838	the mechanical strength	816:838	the mechanical strength of films	816:847	The addition of KGM improved the mechanical strength of films significantly.
31226371	0	6	theme	konjac	104:109	arg1	films					134:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	1	7	theme	gallic	313:318	arg1	acid					320:323	gallic acid	313:323	gallic acid	313:323	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	7	8	theme	films	923:927	arg1	capacity					899:906	the release capacity	887:906	the release capacity of the blended films	887:927	Moreover, KGM improved the release capacity of the blended films, while enhancing antimicrobial activity against Escherichia coli and Staphylococcus aureus.
31226371	3	9	theme	blends	492:497	arg1	stability					475:483	Thermal stability	467:483	Thermal stability of the blends	467:497	Thermal stability of the blends was higher than pure GG, due to Ca2+ crosslinking between GG and KGM.
31226371	0	10	theme	crosslinking	91:102	arg1	films					134:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	10	11	theme	improved	1266:1273	arg1	properties					1275:1284	improved properties	1266:1284	improved properties	1266:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	8	12	from	films	1075:1079	arg1	embedded					1063:1070	embedded	1063:1070	embedded	1063:1070	The antioxidant properties of gallic acid embedded in films were measured.
31226371	0	13	theme	gum	130:132	arg1	films					134:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	6	14	theme	films	843:847	arg1	strength					831:838	the mechanical strength	816:838	the mechanical strength of films	816:847	The addition of KGM improved the mechanical strength of films significantly.
31226371	2	15	dep	transform	399:407	arg1	infrared					409:416	infrared	409:416	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD)	399:464	Structure was analyzed by using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
31226371	9	16	theme	best	1157:1160	arg1	properties					1162:1171	the best properties	1153:1171	the best properties	1153:1171	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	1	17	theme	konjac	192:197	arg1	matrix					242:247	a matrix	240:247	a matrix	240:247	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	17	theme	konjac	192:197	arg1	gum					228:230	gellan gum	221:230	gellan gum (GG)	221:235	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	17	theme	konjac	192:197	arg1	KGM					212:214	KGM	212:214	KGM	212:214	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	17	theme	konjac	192:197	arg1	glucomannan					199:209	konjac glucomannan	192:209	konjac glucomannan (KGM)	192:215	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	17	theme	konjac	192:197	arg1	agent					352:356	a natural antibacterial agent	328:356	a natural antibacterial agent	328:356	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	0	18	theme	glucomannan/gellan	111:128	arg1	films					134:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Ca2+ crosslinking konjac glucomannan/gellan gum films	86:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	2	19	dep	Fourier	391:397	arg1	transform					399:407	transform	399:407	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD)	399:464	Structure was analyzed by using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
31226371	7	20	theme	release	891:897	arg1	capacity					899:906	the release capacity	887:906	the release capacity of the blended films	887:927	Moreover, KGM improved the release capacity of the blended films, while enhancing antimicrobial activity against Escherichia coli and Staphylococcus aureus.
31226371	8	21	from	embedded	1063:1070	arg1	films					1075:1079	films	1075:1079	films	1075:1079	The antioxidant properties of gallic acid embedded in films were measured.
31226371	10	22	theme	films	1255:1259	arg1	synthesis					1223:1231	synthesis	1223:1231	synthesis of GG-based packaging films with improved properties	1223:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	1	23	theme	natural	330:336	arg1	gum					228:230	gellan gum	221:230	gellan gum (GG)	221:235	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	23	theme	natural	330:336	arg1	agent					352:356	a natural antibacterial agent	328:356	a natural antibacterial agent	328:356	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	23	theme	natural	330:336	arg1	glucomannan					199:209	konjac glucomannan	192:209	konjac glucomannan (KGM)	192:215	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	3	24	theme	pure	515:518	arg1	GG					520:521	pure GG	515:521	pure GG	515:521	Thermal stability of the blends was higher than pure GG, due to Ca2+ crosslinking between GG and KGM.
31226371	10	25	theme	packaging	1245:1253	arg1	films					1255:1259	GG-based packaging films	1236:1259	GG-based packaging films with improved properties	1236:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	4	26	theme	vapor	599:603	arg1	permeability					605:616	water vapor permeability	593:616	water vapor permeability	593:616	Water contact angle and water vapor permeability were analyzed to determine hydrophobicity of films.
31226371	8	27	theme	antioxidant	1025:1035	arg1	properties					1037:1046	The antioxidant properties	1021:1046	The antioxidant properties of gallic acid embedded in films	1021:1079	The antioxidant properties of gallic acid embedded in films were measured.
31226371	4	28	theme	water	593:597	arg1	permeability					605:616	water vapor permeability	593:616	water vapor permeability	593:616	Water contact angle and water vapor permeability were analyzed to determine hydrophobicity of films.
31226371	0	29	theme	packaging	29:37	arg1	materials					39:47	antimicrobial packaging materials	15:47	antimicrobial packaging materials	15:47	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	9	30	contain	containing	1112:1121	arg2	Ca-KG7					1135:1140	Ca-KG7	1135:1140	Ca-KG7	1135:1140	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	9	30	contain	containing	1112:1121	arg2	KGM					1130:1132	70 wt% KGM	1123:1132	70 wt% KGM (Ca-KG7)	1123:1141	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	9	30	contain	containing	1112:1121	arg1	films					1106:1110	Composite films	1096:1110	Composite films containing 70 wt% KGM (Ca-KG7)	1096:1141	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	8	31	theme	acid	1058:1061	arg1	properties					1037:1046	The antioxidant properties	1021:1046	The antioxidant properties of gallic acid embedded in films	1021:1079	The antioxidant properties of gallic acid embedded in films were measured.
31226371	0	32	theme	antimicrobial	15:27	arg1	materials					39:47	antimicrobial packaging materials	15:47	antimicrobial packaging materials	15:47	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	8	33	theme	gallic	1051:1056	arg1	acid					1058:1061	gallic acid	1051:1061	gallic acid embedded in films	1051:1079	The antioxidant properties of gallic acid embedded in films were measured.
31226371	1	34	theme	antibacterial	338:350	arg1	gum					228:230	gellan gum	221:230	gellan gum (GG)	221:235	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	34	theme	antibacterial	338:350	arg1	agent					352:356	a natural antibacterial agent	328:356	a natural antibacterial agent	328:356	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	34	theme	antibacterial	338:350	arg1	glucomannan					199:209	konjac glucomannan	192:209	konjac glucomannan (KGM)	192:215	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	5	35	theme	KGM	774:776	arg1	content					778:784	KGM content	774:784	KGM content	774:784	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	4	36	theme	contact	575:581	arg1	angle					583:587	Water contact angle	569:587	Water contact angle	569:587	Water contact angle and water vapor permeability were analyzed to determine hydrophobicity of films.
31226371	1	37	theme	gellan	221:226	arg1	matrix					242:247	a matrix	240:247	a matrix	240:247	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	37	theme	gellan	221:226	arg1	agent					352:356	a natural antibacterial agent	328:356	a natural antibacterial agent	328:356	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	37	theme	gellan	221:226	arg1	glucomannan					199:209	konjac glucomannan	192:209	konjac glucomannan (KGM)	192:215	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	37	theme	gellan	221:226	arg1	GG					233:234	GG	233:234	GG	233:234	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	1	37	theme	gellan	221:226	arg1	gum					228:230	gellan gum	221:230	gellan gum (GG)	221:235	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	0	38	theme	materials	39:47	arg1	Development					0:10	Development	0:10	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.	0:139	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	8	39	theme	embedded	1063:1070	arg1	acid					1058:1061	gallic acid	1051:1061	gallic acid embedded in films	1051:1079	The antioxidant properties of gallic acid embedded in films were measured.
31226371	3	40	theme	Ca2+	531:534	arg1	crosslinking					536:547	Ca2+ crosslinking	531:547	Ca2+ crosslinking between GG and KGM	531:566	Thermal stability of the blends was higher than pure GG, due to Ca2+ crosslinking between GG and KGM.
31226371	9	41	theme	Composite	1096:1104	arg1	films					1106:1110	Composite films	1096:1110	Composite films containing 70 wt% KGM (Ca-KG7)	1096:1141	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	4	42	theme	Water	569:573	arg1	angle					583:587	Water contact angle	569:587	Water contact angle	569:587	Water contact angle and water vapor permeability were analyzed to determine hydrophobicity of films.
31226371	5	43	theme	blended	745:751	arg1	films					753:757	blended films	745:757	blended films	745:757	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	5	44	theme	surface	706:712	arg1	compactness					714:724	surface compactness	706:724	surface compactness	706:724	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	5	45	theme	films	753:757	arg1	homogeneity					730:740	homogeneity	730:740	homogeneity	730:740	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	5	45	theme	films	753:757	arg1	compactness					714:724	surface compactness	706:724	surface compactness	706:724	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	1	46	theme	cross-linking	288:300	arg1	agent					302:306	a cross-linking agent	286:306	a cross-linking agent	286:306	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	9	47	theme	70 wt	1123:1127	arg1	KGM					1130:1132	70 wt% KGM	1123:1132	70 wt% KGM (Ca-KG7)	1123:1141	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	9	47	theme	70 wt	1123:1127	arg1	Ca-KG7					1135:1140	Ca-KG7	1135:1140	Ca-KG7	1135:1140	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	1	48	theme	Antibacterial	141:153	arg1	films					155:159	Antibacterial films	141:159	Antibacterial films	141:159	Antibacterial films were prepared by incorporating konjac glucomannan (KGM) and gellan gum (GG) as a matrix, glycerin as a plasticizer, CaCl2 as a cross-linking agent, and gallic acid as a natural antibacterial agent.
31226371	10	49	theme	alternative	1200:1210	arg1	method					1212:1217	an alternative method	1197:1217	an alternative method for synthesis of GG-based packaging films with improved properties	1197:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	5	50	gly	homogeneity	730:740	arg1	films					753:757	blended films	745:757	blended films	745:757	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31226371	9	51	theme	%	1128:1128	arg1	KGM					1130:1132	70 wt% KGM	1123:1132	70 wt% KGM (Ca-KG7)	1123:1141	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	9	51	theme	%	1128:1128	arg1	Ca-KG7					1135:1140	Ca-KG7	1135:1140	Ca-KG7	1135:1140	Composite films containing 70 wt% KGM (Ca-KG7) displayed the best properties.
31226371	4	52	theme	films	663:667	arg1	hydrophobicity					645:658	hydrophobicity	645:658	hydrophobicity of films	645:667	Water contact angle and water vapor permeability were analyzed to determine hydrophobicity of films.
31226371	0	53	theme	acid	76:79	arg1	incorporation					52:64	incorporation	52:64	incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films	52:138	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	7	54	theme	antimicrobial	946:958	arg1	activity					960:967	antimicrobial activity	946:967	antimicrobial activity against Escherichia coli and Staphylococcus aureus	946:1018	Moreover, KGM improved the release capacity of the blended films, while enhancing antimicrobial activity against Escherichia coli and Staphylococcus aureus.
31226371	3	55	theme	Thermal	467:473	arg1	stability					475:483	Thermal stability	467:483	Thermal stability of the blends	467:497	Thermal stability of the blends was higher than pure GG, due to Ca2+ crosslinking between GG and KGM.
31226371	0	56	theme	gallic	69:74	arg1	acid					76:79	gallic acid	69:79	gallic acid	69:79	Development of antimicrobial packaging materials by incorporation of gallic acid into Ca2+ crosslinking konjac glucomannan/gellan gum films.
31226371	10	57	theme	GG-based	1236:1243	arg1	films					1255:1259	GG-based packaging films	1236:1259	GG-based packaging films with improved properties	1236:1284	These findings suggest an alternative method for synthesis of GG-based packaging films with improved properties.
31226371	5	58	theme	Morphological	670:682	arg1	studies					684:690	Morphological studies	670:690	Morphological studies	670:690	Morphological studies revealed that surface compactness and homogeneity of blended films increased with KGM content.
31726129	2	0	theme	Electron	484:491	arg1	Microscopy					493:502	Scanning Electron Microscopy	475:502	Scanning Electron Microscopy (SEM)	475:508	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	0	theme	Electron	484:491	arg1	SEM					505:507	SEM	505:507	SEM	505:507	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	3	1	theme	present	691:697	arg1	groups					684:689	the funcitonal groups	669:689	the funcitonal groups present in the composite	669:714	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	1	2	theme	CMC	165:167	arg1	/PVP					194:197	CMC (Carboxymethyl cellulose)/PVP	165:197	CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone)	165:221	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	2	theme	CMC	165:167	arg1	pyrrolidone					210:220	Polyvinyl pyrrolidone	200:220	Polyvinyl pyrrolidone	200:220	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	2	3	theme	X-ray	399:403	arg1	XRD					418:420	XRD	418:420	XRD	418:420	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	3	theme	X-ray	399:403	arg1	diffraction					405:415	powder X-ray diffraction	392:415	powder X-ray diffraction (XRD)	392:421	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	7	4	dep	value	1334:1338	arg1	%					1351:1351	less than 3%	1340:1351	hemolysis value less than 3%	1324:1351	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	1	5	dep	HAP	112:114	arg1	Manganese					126:134	Manganese	126:134	Manganese	126:134	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	5	dep	HAP	112:114	arg1	Zinc					117:120	Zinc	117:120	Zinc	117:120	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	2	6	theme	Energy	515:520	arg1	Analysis					539:546	Energy Dispersive X-Ray Analysis	515:546	Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology	515:626	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	6	theme	Energy	515:520	arg1	EDAX					549:552	EDAX	549:552	EDAX	549:552	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	3	7	theme	funcitonal	673:682	arg1	groups					684:689	the funcitonal groups	669:689	the funcitonal groups present in the composite	669:714	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	2	8	theme	phase	570:574	arg1	formation					576:584	the phase formation	566:584	the phase formation	566:584	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	9	theme	porosity	1063:1070	arg1	porosity					1063:1070	porosity	1063:1070	porosity	1063:1070	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	9	theme	porosity	1063:1070	arg1	%					1058:1058	98%	1056:1058	98% of porosity	1056:1070	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	7	10	theme	hemolysis	1324:1332	arg1	value					1334:1338	hemolysis value	1324:1338	hemolysis value less than 3%	1324:1351	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	4	11	theme	fiber	739:743	arg1	formation					745:753	the fiber formation	735:753	the fiber formation	735:753	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	2	12	theme	prepared	347:354	arg1	composites					356:365	The prepared composites	343:365	The prepared composites	343:365	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	5	13	theme	prepared	906:913	arg1	composites					915:924	the prepared composites	902:924	the prepared composites	902:924	The physical properties like porosity, swelling and tensile strength was studied for the prepared composites.
31726129	2	14	theme	surface	609:615	arg1	morphology					617:626	surface morphology	609:626	surface morphology	609:626	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	15	theme	HAP	981:983	arg1	composite					985:993	Zn-Mn HAP composite	975:993	Zn-Mn HAP composite	975:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	16	theme	least	1077:1081	arg1	swelling					1083:1090	least swelling	1077:1090	least swelling	1077:1090	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	1	17	theme	Carboxymethyl	170:182	arg1	/PVP					194:197	CMC (Carboxymethyl cellulose)/PVP	165:197	CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone)	165:221	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	17	theme	Carboxymethyl	170:182	arg1	pyrrolidone					210:220	Polyvinyl pyrrolidone	200:220	Polyvinyl pyrrolidone	200:220	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	6	18	theme	/CMC/PVP	947:954	arg1	0.1 M					927:931	0.1 M	927:931	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite)	927:994	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	19	dep	/CMC/PVP	947:954	arg1	composite					985:993	Zn-Mn HAP composite	975:993	Zn-Mn HAP composite	975:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	19	dep	/CMC/PVP	947:954	arg1	%					970:970	20, 40, 60 wt%	957:970	20, 40, 60 wt% of Zn-Mn HAP composite	957:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	1	20	theme	cellulose	184:192	arg1	/PVP					194:197	CMC (Carboxymethyl cellulose)/PVP	165:197	CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone)	165:221	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	20	theme	cellulose	184:192	arg1	pyrrolidone					210:220	Polyvinyl pyrrolidone	200:220	Polyvinyl pyrrolidone	200:220	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	2	21	theme	Scanning	475:482	arg1	Microscopy					493:502	Scanning Electron Microscopy	475:502	Scanning Electron Microscopy (SEM)	475:508	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	21	theme	Scanning	475:482	arg1	SEM					505:507	SEM	505:507	SEM	505:507	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	22	theme	physical	1008:1015	arg1	properties					1017:1026	good physical properties	1003:1026	good physical properties	1003:1026	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	1	23	theme	Zn-Mn	106:110	arg1	HAP					112:114	Zn-Mn HAP	106:114	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite)	106:162	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	2	24	theme	infrared	442:449	arg1	FTIR					465:468	FTIR	465:468	FTIR	465:468	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	24	theme	infrared	442:449	arg1	spectroscopy					451:462	Fourier-transform infrared spectroscopy	424:462	Fourier-transform infrared spectroscopy (FTIR)	424:469	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	25	theme	tensile	1100:1106	arg1	strength					1108:1115	the tensile strength	1096:1115	the tensile strength	1096:1115	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	25	theme	tensile	1100:1106	arg1	67 MPa					1136:1141	67 MPa	1136:1141	67 MPa	1136:1141	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	2	26	theme	functional	587:596	arg1	groups					598:603	functional groups	587:603	functional groups	587:603	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	4	27	theme	SEM	717:719	arg1	images					721:726	SEM images	717:726	SEM images	717:726	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	2	28	theme	Fourier-transform	424:440	arg1	FTIR					465:468	FTIR	465:468	FTIR	465:468	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	28	theme	Fourier-transform	424:440	arg1	spectroscopy					451:462	Fourier-transform infrared spectroscopy	424:462	Fourier-transform infrared spectroscopy (FTIR)	424:469	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	29	theme	HAP	943:945	arg1	/CMC/PVP					947:954	(Zn-Mn HAP)/CMC/PVP	936:954	(Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite)	936:994	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	7	30	theme	composite	1266:1274	arg1	0.1 M					1237:1241	0.1 M	1237:1241	0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1237:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	6	31	theme	good	1003:1006	arg1	properties					1017:1026	good physical properties	1003:1026	good physical properties	1003:1026	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	32	theme	composite	985:993	arg1	composite					985:993	Zn-Mn HAP composite	975:993	Zn-Mn HAP composite	975:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	32	theme	composite	985:993	arg1	%					970:970	20, 40, 60 wt%	957:970	20, 40, 60 wt% of Zn-Mn HAP composite	957:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	6	33	theme	Zn-Mn	937:941	arg1	/CMC/PVP					947:954	(Zn-Mn HAP)/CMC/PVP	936:954	(Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite)	936:994	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	3	34	theme	FTIR	629:632	arg1	spectra					634:640	FTIR spectra	629:640	FTIR spectra of the composite	629:657	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	7	35	theme	Highest	1144:1150	arg1	zone					1152:1155	Highest zone	1144:1155	Highest zone of inhibition	1144:1169	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	1	36	theme	Polyvinyl	200:208	arg1	/PVP					194:197	CMC (Carboxymethyl cellulose)/PVP	165:197	CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone)	165:221	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	36	theme	Polyvinyl	200:208	arg1	pyrrolidone					210:220	Polyvinyl pyrrolidone	200:220	Polyvinyl pyrrolidone	200:220	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	0	37	theme	electrospun	54:64	arg1	composite					66:74	hydroxyapatite/CMC/PVP electrospun composite	31:74	hydroxyapatite/CMC/PVP electrospun composite	31:74	Zinc and manganese substituted hydroxyapatite/CMC/PVP electrospun composite for bone repair applications.
31726129	8	38	theme	composite	1416:1424	arg1	biocompatibility					1392:1407	The biocompatibility	1388:1407	The biocompatibility of the composite	1388:1424	The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).
31726129	2	39	theme	X-Ray	533:537	arg1	Analysis					539:546	Energy Dispersive X-Ray Analysis	515:546	Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology	515:626	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	39	theme	X-Ray	533:537	arg1	EDAX					549:552	EDAX	549:552	EDAX	549:552	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	6	40	theme	Zn-Mn	975:979	arg1	composite					985:993	Zn-Mn HAP composite	975:993	Zn-Mn HAP composite	975:993	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	8	41	theme	osteoblast	1452:1461	arg1	HOS					1470:1472	HOS	1470:1472	HOS	1470:1472	The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).
31726129	8	41	theme	osteoblast	1452:1461	arg1	cells					1463:1467	human osteoblast cells	1446:1467	human osteoblast cells (HOS)	1446:1473	The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).
31726129	5	42	theme	physical	821:828	arg1	properties					830:839	The physical properties	817:839	The physical properties like porosity, swelling and tensile strength	817:884	The physical properties like porosity, swelling and tensile strength was studied for the prepared composites.
31726129	0	43	theme	hydroxyapatite/CMC/PVP	31:52	arg1	composite					66:74	hydroxyapatite/CMC/PVP electrospun composite	31:74	hydroxyapatite/CMC/PVP electrospun composite	31:74	Zinc and manganese substituted hydroxyapatite/CMC/PVP electrospun composite for bone repair applications.
31726129	2	44	theme	Dispersive	522:531	arg1	Analysis					539:546	Energy Dispersive X-Ray Analysis	515:546	Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology	515:626	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	44	theme	Dispersive	522:531	arg1	EDAX					549:552	EDAX	549:552	EDAX	549:552	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	7	45	theme	wt	1230:1231	arg1	0.1 M					1237:1241	0.1 M	1237:1241	0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1237:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	45	theme	wt	1230:1231	arg1	%					1232:1232	this 60 wt%	1222:1232	this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1222:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	3	46	from	composite	706:714	arg1	present					691:697	present	691:697	present	691:697	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	7	47	theme	microbial	1196:1204	arg1	organisms					1206:1214	the microbial organisms	1192:1214	the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1192:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	3	48	attach	present	691:697	arg1	composite					706:714	the composite	702:714	the composite	702:714	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	3	48	attach	present	691:697	arg2	groups					684:689	the funcitonal groups	669:689	the funcitonal groups present in the composite	669:714	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	7	49	theme	Zn-Mn	1247:1251	arg1	HAP					1253:1255	Zn-Mn HAP	1247:1255	Zn-Mn HAP	1247:1255	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	49	theme	Zn-Mn	1247:1251	arg1	composite					1266:1274	(Zn-Mn HAP)/CMC/PVP composite	1246:1274	(Zn-Mn HAP)/CMC/PVP composite	1246:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	3	50	from	present	691:697	arg1	composite					706:714	the composite	702:714	the composite	702:714	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	1	51	theme	hydrothermal	288:299	arg1	methods					321:327	hydrothermal and electrospinning methods	288:327	hydrothermal and electrospinning methods respectively	288:340	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	3	52	theme	composite	649:657	arg1	spectra					634:640	FTIR spectra	629:640	FTIR spectra of the composite	629:657	FTIR spectra of the composite confirmed the funcitonal groups present in the composite.
31726129	4	53	theme	fiber	799:803	arg1	structures					805:814	the fiber structures	795:814	the fiber structures	795:814	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	8	54	theme	human	1446:1450	arg1	HOS					1470:1472	HOS	1470:1472	HOS	1470:1472	The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).
31726129	8	54	theme	human	1446:1450	arg1	cells					1463:1467	human osteoblast cells	1446:1467	human osteoblast cells (HOS)	1446:1473	The biocompatibility of the composite was evaluated using human osteoblast cells (HOS).
31726129	7	55	with	hemocompatible	1304:1317	arg1	value					1334:1338	hemolysis value	1324:1338	hemolysis value less than 3%	1324:1351	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	56	theme	0.1 M	1237:1241	arg1	0.1 M					1237:1241	0.1 M	1237:1241	0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1237:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	56	theme	0.1 M	1237:1241	arg1	%					1232:1232	this 60 wt%	1222:1232	this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite	1222:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	57	theme	other	1370:1374	arg1	composites					1376:1385	other composites	1370:1385	other composites	1370:1385	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	58	theme	/CMC/PVP	1257:1264	arg1	HAP					1253:1255	Zn-Mn HAP	1247:1255	Zn-Mn HAP	1247:1255	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	7	58	theme	/CMC/PVP	1257:1264	arg1	composite					1266:1274	(Zn-Mn HAP)/CMC/PVP composite	1246:1274	(Zn-Mn HAP)/CMC/PVP composite	1246:1274	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	1	59	theme	substituted	136:146	arg1	Hydroxyapatite					148:161	substituted Hydroxyapatite	136:161	substituted Hydroxyapatite	136:161	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	5	60	theme	tensile	869:875	arg1	strength					877:884	tensile strength	869:884	tensile strength	869:884	The physical properties like porosity, swelling and tensile strength was studied for the prepared composites.
31726129	6	61	theme	60 wt	1042:1046	arg1	%					1047:1047	the 60 wt%	1038:1047	the 60 wt%	1038:1047	0.1 M of (Zn-Mn HAP)/CMC/PVP (20, 40, 60 wt% of Zn-Mn HAP composite) showed good physical properties, in which the 60 wt% showed 98% of porosity with least swelling and the tensile strength was measured to be 67 MPa.
31726129	0	62	theme	repair	85:90	arg1	applications					92:103	bone repair applications	80:103	bone repair applications	80:103	Zinc and manganese substituted hydroxyapatite/CMC/PVP electrospun composite for bone repair applications.
31726129	4	63	theme	HAP	786:788	arg1	formation					745:753	the fiber formation	735:753	the fiber formation	735:753	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	4	63	theme	HAP	786:788	arg1	incorporation					763:775	the incorporation	759:775	the incorporation of Zn-Mn HAP into the fiber structures	759:814	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	2	64	theme	powder	392:397	arg1	XRD					418:420	XRD	418:420	XRD	418:420	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	2	64	theme	powder	392:397	arg1	diffraction					405:415	powder X-ray diffraction	392:415	powder X-ray diffraction (XRD)	392:421	The prepared composites were characterized using powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) to examine the phase formation, functional groups and surface morphology.
31726129	1	65	theme	Zn-Mn	228:232	arg1	/CMC/PVP					238:245	(Zn-Mn HAP)/CMC/PVP	227:245	(Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M)	227:269	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	65	theme	Zn-Mn	228:232	arg1	0.1 M					264:268	0.1 M	264:268	0.1 M	264:268	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	0	66	theme	bone	80:83	arg1	applications					92:103	bone repair applications	80:103	bone repair applications	80:103	Zinc and manganese substituted hydroxyapatite/CMC/PVP electrospun composite for bone repair applications.
31726129	1	67	theme	electrospinning	305:319	arg1	methods					321:327	hydrothermal and electrospinning methods	288:327	hydrothermal and electrospinning methods respectively	288:340	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	4	68	theme	Zn-Mn	780:784	arg1	HAP					786:788	Zn-Mn HAP	780:788	Zn-Mn HAP	780:788	SEM images showed the fiber formation and the incorporation of Zn-Mn HAP into the fiber structures.
31726129	7	69	theme	inhibition	1160:1169	arg1	zone					1152:1155	Highest zone	1144:1155	Highest zone of inhibition	1144:1169	Highest zone of inhibition was observed against the microbial organisms using this 60 wt% of 0.1 M of (Zn-Mn HAP)/CMC/PVP composite and it was also found to be hemocompatible with hemolysis value less than 3% when compared to other composites.
31726129	1	70	dep	Zinc	117:120	arg1	Hydroxyapatite					148:161	substituted Hydroxyapatite	136:161	substituted Hydroxyapatite	136:161	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	71	theme	HAP	234:236	arg1	/CMC/PVP					238:245	(Zn-Mn HAP)/CMC/PVP	227:245	(Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M)	227:269	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31726129	1	71	theme	HAP	234:236	arg1	0.1 M					264:268	0.1 M	264:268	0.1 M	264:268	Zn-Mn HAP (Zinc and Manganese substituted Hydroxyapatite), CMC (Carboxymethyl cellulose)/PVP (Polyvinyl pyrrolidone) and (Zn-Mn HAP)/CMC/PVP (Zn = Mn = 0.05, 0.1 M) were prepared by hydrothermal and electrospinning methods respectively.
31094049	3	0	theme	cells	757:761	arg1	migration					674:682	migration	674:682	migration	674:682	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	0	theme	cells	757:761	arg1	proliferation					694:706	rapid proliferation	688:706	rapid proliferation	688:706	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	1	1	theme	factors	187:193	arg1	release					165:171	Controlled release	154:171	Controlled release of functional factors	154:193	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	3	2	theme	mesenchymal	711:721	arg1	cells					728:732	mesenchymal stem cells	711:732	mesenchymal stem cells (MSCs)	711:739	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	2	theme	mesenchymal	711:721	arg1	MSCs					735:738	MSCs	735:738	MSCs	735:738	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	4	3	theme	composited	867:876	arg1	system					878:883	our composited system	863:883	our composited system	863:883	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	1	4	theme	in	280:281	arg1	vascularization					288:302	the in situ vascularization	276:302	the in situ vascularization of tissue repair and regeneration	276:336	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	3	5	theme	stem	723:726	arg1	cells					728:732	mesenchymal stem cells	711:732	mesenchymal stem cells (MSCs)	711:739	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	5	theme	stem	723:726	arg1	MSCs					735:738	MSCs	735:738	MSCs	735:738	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	6	theme	stromal	545:551	arg1	SDF-1					576:580	SDF-1	576:580	SDF-1	576:580	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	6	theme	stromal	545:551	arg1	factor-1					566:573	stromal cell-derived factor-1	545:573	stromal cell-derived factor-1 (SDF-1)	545:581	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	7	theme	growth	604:609	arg1	VEGF					619:622	VEGF	619:622	VEGF	619:622	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	7	theme	growth	604:609	arg1	factor					611:616	/vascular endothelial growth factor	582:616	/vascular endothelial growth factor (VEGF)	582:623	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	0	8	theme	tissue	134:139	arg1	engineering					141:151	tissue engineering	134:151	tissue engineering	134:151	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	3	9	theme	endothelial	745:755	arg1	ECs					764:766	ECs	764:766	ECs	764:766	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	9	theme	endothelial	745:755	arg1	cells					757:761	endothelial cells	745:761	endothelial cells (ECs)	745:767	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	4	10	dep	in	826:827	arg1	vivo					829:832	vivo	829:832	vivo	829:832	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	5	11	theme	nanoparticles	975:987	arg1	properties					957:966	the properties	953:966	the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility,	953:1077	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	4	12	dep	in	813:814	arg1	vitro					816:820	vitro	816:820	vitro	816:820	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	4	13	theme	experiments	834:844	arg1	Series					803:808	Series	803:808	Series of in vitro and in vivo experiments	803:844	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	0	14	theme	injectable	3:12	arg1	scaffold					14:21	An injectable scaffold	0:21	An injectable scaffold	0:21	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	3	15	theme	cell-derived	553:564	arg1	SDF-1					576:580	SDF-1	576:580	SDF-1	576:580	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	15	theme	cell-derived	553:564	arg1	factor-1					566:573	stromal cell-derived factor-1	545:573	stromal cell-derived factor-1 (SDF-1)	545:581	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	2	16	theme	biomedical	341:350	arg1	application					352:362	A biomedical application	339:362	A biomedical application	339:362	A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells.
31094049	5	17	theme	selective	1108:1116	arg1	release					1118:1124	the selective release	1104:1124	the selective release	1104:1124	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	3	18	theme	rapid	688:692	arg1	proliferation					694:706	rapid proliferation	688:706	rapid proliferation	688:706	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	1	19	theme	tissue	307:312	arg1	repair					314:319	tissue repair	307:319	tissue repair	307:319	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	5	20	theme	charge	1049:1054	arg1	biocompatibility					1061:1076	biocompatibility	1061:1076	biocompatibility	1061:1076	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	20	theme	charge	1049:1054	arg1	characteristic					1031:1044	characteristic	1031:1044	characteristic of charge	1031:1054	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	20	theme	charge	1049:1054	arg1	micro/nanoscales					1013:1028	micro/nanoscales	1013:1028	micro/nanoscales	1013:1028	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	3	21	theme	/vascular	582:590	arg1	VEGF					619:622	VEGF	619:622	VEGF	619:622	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	21	theme	/vascular	582:590	arg1	factor					611:616	/vascular endothelial growth factor	582:616	/vascular endothelial growth factor (VEGF)	582:623	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	2	22	theme	cells	451:455	arg1	synergy					436:442	the synergy	432:442	the synergy of the cells	432:455	A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells.
31094049	3	23	theme	injectable	480:489	arg1	system					491:496	an injectable system	477:496	an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release	477:800	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	1	24	theme	repair	314:319	arg1	vascularization					288:302	the in situ vascularization	276:302	the in situ vascularization of tissue repair and regeneration	276:336	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	0	25	from	application	100:110	arg1	vascularization					115:129	vascularization	115:129	vascularization in tissue engineering	115:151	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	3	26	theme	endothelial	592:602	arg1	VEGF					619:622	VEGF	619:622	VEGF	619:622	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	26	theme	endothelial	592:602	arg1	factor					611:616	/vascular endothelial growth factor	582:616	/vascular endothelial growth factor (VEGF)	582:623	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	1	27	theme	cells	242:246	arg1	migration					217:225	migration	217:225	migration of therapeutic cells	217:246	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	0	28	theme	temperature-responsive	32:53	arg1	hydrogel					55:62	temperature-responsive hydrogel	32:62	temperature-responsive hydrogel	32:62	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	3	29	dep	hydrogel	532:539	arg1	VEGF					619:622	VEGF	619:622	VEGF	619:622	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	29	dep	hydrogel	532:539	arg1	factor					611:616	/vascular endothelial growth factor	582:616	/vascular endothelial growth factor (VEGF)	582:623	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	5	30	theme	crucial	1086:1092	arg1	roles					1094:1098	crucial roles	1086:1098	crucial roles for the selective release and cells behavior (target migration and rapid proliferation)	1086:1186	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	1	31	theme	regeneration	325:336	arg1	vascularization					288:302	the in situ vascularization	276:302	the in situ vascularization of tissue repair and regeneration	276:336	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	3	32	link	cell-derived	553:564	arg1	SDF-1					576:580	SDF-1	576:580	SDF-1	576:580	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	32	link	cell-derived	553:564	arg1	factor-1					566:573	stromal cell-derived factor-1	545:573	stromal cell-derived factor-1 (SDF-1)	545:581	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	33	theme	nanoparticles	650:662	arg1	types					641:645	two types	637:645	two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release	637:800	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	34	theme	SDF-1/VEGF	783:792	arg1	release					794:800	selective SDF-1/VEGF release	773:800	selective SDF-1/VEGF release	773:800	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	2	35	theme	selective	377:385	arg1	release					387:393	the selective release	373:393	the selective release of multiple factors which will guide the synergy of the cells	373:455	A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells.
31094049	5	36	theme	target	1146:1151	arg1	migration					1153:1161	target migration	1146:1161	target migration	1146:1161	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	37	theme	rapid	1167:1171	arg1	proliferation					1173:1185	rapid proliferation	1167:1185	rapid proliferation	1167:1185	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	38	theme	cells	1130:1134	arg1	behavior					1136:1143	cells behavior	1130:1143	cells behavior (target migration and rapid proliferation)	1130:1186	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	39	dep	behavior	1136:1143	arg1	migration					1153:1161	target migration	1146:1161	target migration	1146:1161	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	5	39	dep	behavior	1136:1143	arg1	proliferation					1173:1185	rapid proliferation	1167:1185	rapid proliferation	1167:1185	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	4	40	theme	in	826:827	arg1	experiments					834:844	in vitro and in vivo experiments	813:844	in vitro and in vivo experiments	813:844	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	5	41	dep	nanoparticles	975:987	arg1	the					971:973	the	971:973	the	971:973	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	3	42	theme	temperature-responsive	509:530	arg1	hydrogel					532:539	a temperature-responsive hydrogel	507:539	a temperature-responsive hydrogel	507:539	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	43	theme	selective	773:781	arg1	release					794:800	selective SDF-1/VEGF release	773:800	selective SDF-1/VEGF release	773:800	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	5	44	theme	hydrogel	993:1000	arg1	properties					957:966	the properties	953:966	the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility,	953:1077	In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).
31094049	1	45	theme	Controlled	154:163	arg1	release					165:171	Controlled release	154:171	Controlled release of functional factors	154:193	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	0	46	from	vascularization	115:129	arg1	engineering					141:151	tissue engineering	134:151	tissue engineering	134:151	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	1	47	theme	functional	176:185	arg1	factors					187:193	functional factors	176:193	functional factors	176:193	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	1	48	theme	crucial	260:266	arg1	role					268:271	a crucial role	258:271	a crucial role	258:271	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	1	49	dep	in	280:281	arg1	situ					283:286	situ	283:286	situ	283:286	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	4	50	theme	in	813:814	arg1	experiments					834:844	in vitro and in vivo experiments	813:844	in vitro and in vivo experiments	813:844	Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization.
31094049	1	51	theme	therapeutic	230:240	arg1	cells					242:246	therapeutic cells	230:246	therapeutic cells	230:246	Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration.
31094049	2	52	theme	factors	407:413	arg1	release					387:393	the selective release	373:393	the selective release of multiple factors which will guide the synergy of the cells	373:455	A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells.
31094049	0	53	theme	factor-loaded	68:80	arg1	nanoparticles					82:94	factor-loaded nanoparticles	68:94	factor-loaded nanoparticles	68:94	An injectable scaffold based on temperature-responsive hydrogel and factor-loaded nanoparticles for application in vascularization in tissue engineering.
31094049	3	54	theme	cells	728:732	arg1	migration					674:682	migration	674:682	migration	674:682	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	3	54	theme	cells	728:732	arg1	proliferation					694:706	rapid proliferation	688:706	rapid proliferation	688:706	Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release.
31094049	2	55	theme	multiple	398:405	arg1	factors					407:413	multiple factors	398:413	multiple factors	398:413	A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells.
30504145	8	0	dep	Nasutitermes	1405:1416	arg1	takasagoensis					1418:1430	Nasutitermes takasagoensis	1405:1430	Nasutitermes takasagoensis	1405:1430	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	6	1	theme	fiber-associated	1133:1148	arg1	community					1160:1168	the fiber-associated bacterial community	1129:1168	the fiber-associated bacterial community	1129:1168	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	4	2	theme	wood	625:628	arg1	fibers					630:635	the wood fibers	621:635	the wood fibers in the hindgut paunch	621:657	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	2	3	theme	symbionts	505:513	arg1	contributions					469:481	the contributions	465:481	the contributions of host and bacterial symbionts to lignocellulose degradation	465:543	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	9	4	theme	major	1758:1762	arg1	role					1764:1767	The major role	1754:1767	The major role of spirochetes in xylan degradation	1754:1803	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	9	4	theme	major	1758:1762	arg1	unprecedented					1808:1820	unprecedented	1808:1820	unprecedented	1808:1820	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	6	5	theme	bacterial	1150:1158	arg1	community					1160:1168	the fiber-associated bacterial community	1129:1168	the fiber-associated bacterial community	1129:1168	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	8	6	theme	GH11	1397:1400	arg1	transcripts					1382:1392	the most abundant transcripts	1364:1392	the most abundant transcripts of GH11 in Nasutitermes takasagoensis	1364:1430	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	0	7	theme	higher	106:111	arg1	termites					113:120	wood-feeding higher termites	93:120	wood-feeding higher termites	93:120	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	8	8	theme	homologs	1606:1613	arg1	context					1591:1597	the genomic context	1579:1597	the genomic context of the homologs	1579:1613	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	8	theme	homologs	1606:1613	arg1	microscopy					1516:1525	immunofluorescence microscopy	1497:1525	immunofluorescence microscopy	1497:1525	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	8	theme	homologs	1606:1613	arg1	binning					1542:1548	compositional binning	1528:1548	compositional binning of metagenomics contigs	1528:1572	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	9	9	from	clades	1865:1870	arg1	hindgut					1879:1885	the hindgut	1875:1885	the hindgut of wood-feeding higher termites	1875:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	4	10	theme	unique	800:805	arg1	lineages					807:814	unique lineages	800:814	unique lineages of Spirochaetes	800:830	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	6	11	theme	Metatranscriptomic	1019:1036	arg1	analysis					1038:1045	Metatranscriptomic analysis	1019:1045	Metatranscriptomic analysis	1019:1045	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	0	12	theme	wood-feeding	93:104	arg1	termites					113:120	wood-feeding higher termites	93:120	wood-feeding higher termites	93:120	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	1	13	from	community	321:329	arg1	compartment					346:356	the hindgut compartment	334:356	the hindgut compartment	334:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	3	14	theme	Nasutitermes	587:598	arg1	studies					576:582	Our previous studies	563:582	Our previous studies of Nasutitermes	563:598	Our previous studies of Nasutitermes spp.
30504145	7	15	theme	predominant	1334:1344	arg1	homolog					1346:1352	the predominant homolog	1330:1352	the predominant homolog	1330:1352	The substrate specificity was further confirmed by heterologous expression of the gene encoding the predominant homolog.
30504145	7	16	theme	substrate	1238:1246	arg1	specificity					1248:1258	The substrate specificity	1234:1258	The substrate specificity	1234:1258	The substrate specificity was further confirmed by heterologous expression of the gene encoding the predominant homolog.
30504145	7	17	theme	gene	1316:1319	arg1	expression					1298:1307	heterologous expression	1285:1307	heterologous expression of the gene encoding the predominant homolog	1285:1352	The substrate specificity was further confirmed by heterologous expression of the gene encoding the predominant homolog.
30504145	9	18	theme	spirochetes	1772:1782	arg1	role					1764:1767	The major role	1754:1767	The major role of spirochetes in xylan degradation	1754:1803	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	9	18	theme	spirochetes	1772:1782	arg1	unprecedented					1808:1820	unprecedented	1808:1820	unprecedented	1808:1820	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	3	19	theme	previous	567:574	arg1	studies					576:582	Our previous studies	563:582	Our previous studies of Nasutitermes	563:598	Our previous studies of Nasutitermes spp.
30504145	6	20	theme	transcripts	1104:1114	arg1	majority					1067:1074	the majority	1063:1074	the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community	1063:1168	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	2	21	theme	diverse	410:416	arg1	genomes					363:369	The genomes	359:369	The genomes of the bacterial gut microbiota	359:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	21	theme	diverse	410:416	arg1	enzymes					452:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	0	22	theme	termites	113:120	arg1	hindgut					82:88	the hindgut	78:88	the hindgut of wood-feeding higher termites	78:120	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	9	23	theme	hemicelluloses	1956:1969	arg1	breakdown					1943:1951	the breakdown	1939:1951	the breakdown of hemicelluloses	1939:1969	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	8	24	theme	intestinal	1731:1740	arg1	microbiota					1742:1751	the intestinal microbiota	1727:1751	the intestinal microbiota	1727:1751	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	25	theme	horizontal	1696:1705	arg1	transfer					1712:1719	horizontal gene transfer	1696:1719	horizontal gene transfer among the intestinal microbiota	1696:1751	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	1	26	theme	family	194:199	arg1	Termitidae					201:210	family Termitidae	194:210	family Termitidae	194:210	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	26	theme	family	194:199	arg1	termites					184:191	wood-feeding higher termites	164:191	wood-feeding higher termites (family Termitidae)	164:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	9	27	theme	prominent	1921:1929	arg1	part					1931:1934	a prominent part	1919:1934	a prominent part	1919:1934	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	0	28	from	agents	39:44	arg1	hindgut					82:88	the hindgut	78:88	the hindgut of wood-feeding higher termites	78:120	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	9	29	theme	xylan	1787:1791	arg1	degradation					1793:1803	xylan degradation	1787:1803	xylan degradation	1787:1803	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	8	30	theme	gene	1707:1710	arg1	transfer					1712:1719	horizontal gene transfer	1696:1719	horizontal gene transfer among the intestinal microbiota	1696:1751	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	2	31	theme	microbiota	392:401	arg1	genomes					363:369	The genomes	359:369	The genomes of the bacterial gut microbiota	359:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	31	theme	microbiota	392:401	arg1	enzymes					452:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	0	32	theme	Fiber-associated	0:15	arg1	spirochetes					17:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	0	32	theme	Fiber-associated	0:15	arg1	agents					39:44	major agents	33:44	major agents of hemicellulose degradation	33:73	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	9	33	from	role	1764:1767	arg1	degradation					1793:1803	xylan degradation	1787:1803	xylan degradation	1787:1803	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	8	34	theme	genomic	1583:1589	arg1	context					1591:1597	the genomic context	1579:1597	the genomic context of the homologs	1579:1613	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	2	35	theme	gut	388:390	arg1	microbiota					392:401	the bacterial gut microbiota	374:401	the bacterial gut microbiota	374:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	0	36	theme	major	33:37	arg1	spirochetes					17:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	0	36	theme	major	33:37	arg1	agents					39:44	major agents	33:44	major agents of hemicellulose degradation	33:73	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	8	37	theme	abundant	1373:1380	arg1	transcripts					1382:1392	the most abundant transcripts	1364:1392	the most abundant transcripts of GH11 in Nasutitermes takasagoensis	1364:1430	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	9	38	theme	higher	1903:1908	arg1	termites					1910:1917	wood-feeding higher termites	1890:1917	wood-feeding higher termites	1890:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	1	39	from	digestion	133:141	arg1	Termitidae					201:210	family Termitidae	194:210	family Termitidae	194:210	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	39	from	digestion	133:141	arg1	termites					184:191	wood-feeding higher termites	164:191	wood-feeding higher termites (family Termitidae)	164:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	6	40	theme	hydrolase	1089:1097	arg1	transcripts					1104:1114	glycoside hydrolase (GH) transcripts	1079:1114	glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community	1079:1168	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	8	41	theme	metagenomics	1553:1564	arg1	contigs					1566:1572	metagenomics contigs	1553:1572	metagenomics contigs	1553:1572	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	1	42	dep	wood-feeding	164:175	arg1	higher					177:182	higher	177:182	higher	177:182	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	2	43	theme	host	486:489	arg1	contributions					469:481	the contributions	465:481	the contributions of host and bacterial symbionts to lignocellulose degradation	465:543	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	44	theme	hemicellulolytic	435:450	arg1	genomes					363:369	The genomes	359:369	The genomes of the bacterial gut microbiota	359:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	44	theme	hemicellulolytic	435:450	arg1	enzymes					452:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	45	theme	bacterial	495:503	arg1	symbionts					505:513	bacterial symbionts	495:513	bacterial symbionts	495:513	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	1	46	theme	dense	305:309	arg1	community					321:329	a dense bacterial community	303:329	a dense bacterial community in the hindgut compartment	303:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	47	from	cellulases	265:274	arg1	compartment					346:356	the hindgut compartment	334:356	the hindgut compartment	334:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	5	48	theme	xylan	878:882	arg1	degradation					863:873	the degradation	859:873	the degradation of xylan, the major component of hemicellulose,	859:921	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	5	48	theme	xylan	878:882	arg1	restricted					926:935	restricted	926:935	restricted	926:935	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	8	49	theme	Firmicutes	1485:1494	arg1	homologs					1473:1480	their homologs	1467:1480	their homologs of Firmicutes	1467:1494	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	1	50	theme	two-step	218:225	arg1	digestion					133:141	Symbiotic digestion	123:141	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae)	123:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	50	theme	two-step	218:225	arg1	process					227:233	a two-step process	216:233	a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment	216:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	4	51	theme	uncultured	698:707	arg1	members					709:715	uncultured members	698:715	uncultured members	698:715	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	1	52	theme	bacterial	311:319	arg1	community					321:329	a dense bacterial community	303:329	a dense bacterial community in the hindgut compartment	303:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	4	53	from	fibers	630:635	arg1	paunch					652:657	the hindgut paunch	640:657	the hindgut paunch	640:657	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	9	54	theme	termites	1910:1917	arg1	hindgut					1879:1885	the hindgut	1875:1885	the hindgut of wood-feeding higher termites	1875:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	2	55	theme	cellulolytic	418:429	arg1	genomes					363:369	The genomes	359:369	The genomes of the bacterial gut microbiota	359:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	2	55	theme	cellulolytic	418:429	arg1	enzymes					452:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	diverse cellulolytic and hemicellulolytic enzymes	410:458	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	0	56	theme	degradation	63:73	arg1	spirochetes					17:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes	0:27	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	0	56	theme	degradation	63:73	arg1	agents					39:44	major agents	33:44	major agents of hemicellulose degradation	33:73	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	5	57	theme	hemicellulose	908:920	arg1	xylan					878:882	xylan	878:882	xylan	878:882	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	5	57	theme	hemicellulose	908:920	arg1	component					895:903	the major component	885:903	the major component of hemicellulose	885:920	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	8	58	theme	contigs	1566:1572	arg1	context					1591:1597	the genomic context	1579:1597	the genomic context of the homologs	1579:1613	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	58	theme	contigs	1566:1572	arg1	microscopy					1516:1525	immunofluorescence microscopy	1497:1525	immunofluorescence microscopy	1497:1525	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	58	theme	contigs	1566:1572	arg1	binning					1542:1548	compositional binning	1528:1548	compositional binning of metagenomics contigs	1528:1572	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	8	59	theme	immunofluorescence	1497:1514	arg1	microscopy					1516:1525	immunofluorescence microscopy	1497:1525	immunofluorescence microscopy	1497:1525	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	1	60	theme	Symbiotic	123:131	arg1	digestion					133:141	Symbiotic digestion	123:141	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae)	123:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	60	theme	Symbiotic	123:131	arg1	process					227:233	a two-step process	216:233	a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment	216:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	0	61	theme	hemicellulose	49:61	arg1	degradation					63:73	hemicellulose degradation	49:73	hemicellulose degradation	49:73	Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.
30504145	9	62	theme	fiber-associated	1838:1853	arg1	clades					1865:1870	the fiber-associated Treponema clades	1834:1870	the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites	1834:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	6	63	theme	family	1180:1185	arg1	GH11					1187:1190	family GH11	1180:1190	family GH11	1180:1190	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	8	64	from	transcripts	1382:1392	arg1	Nasutitermes					1405:1416	Nasutitermes	1405:1416	Nasutitermes	1405:1416	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	9	65	theme	Treponema	1855:1863	arg1	clades					1865:1870	the fiber-associated Treponema clades	1834:1870	the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites	1834:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	4	66	theme	Fibrobacteres	720:732	arg1	members					709:715	uncultured members	698:715	uncultured members	698:715	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	4	67	theme	cellulose	765:773	arg1	degradation					775:785	cellulose degradation	765:785	cellulose degradation	765:785	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	4	68	theme	hindgut	644:650	arg1	paunch					652:657	the hindgut paunch	640:657	the hindgut paunch	640:657	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	4	69	theme	Spirochaetes	819:830	arg1	lineages					807:814	unique lineages	800:814	unique lineages of Spirochaetes	800:830	documented that the wood fibers in the hindgut paunch are consistently colonized not only by uncultured members of Fibrobacteres, which have been implicated in cellulose degradation, but also by unique lineages of Spirochaetes.
30504145	1	70	theme	lignocellulose	146:159	arg1	digestion					133:141	Symbiotic digestion	123:141	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae)	123:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	70	theme	lignocellulose	146:159	arg1	process					227:233	a two-step process	216:233	a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment	216:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	5	71	theme	hindgut	944:950	arg1	compartment					952:962	the hindgut compartment	940:962	the hindgut compartment	940:962	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	7	72	theme	heterologous	1285:1296	arg1	expression					1298:1307	heterologous expression	1285:1307	heterologous expression of the gene encoding the predominant homolog	1285:1352	The substrate specificity was further confirmed by heterologous expression of the gene encoding the predominant homolog.
30504145	6	73	theme	glycoside	1079:1087	arg1	GH					1100:1101	GH	1100:1101	GH	1100:1101	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	6	73	theme	glycoside	1079:1087	arg1	hydrolase					1089:1097	glycoside hydrolase	1079:1097	glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community	1079:1168	Metatranscriptomic analysis documented that the majority of glycoside hydrolase (GH) transcripts expressed by the fiber-associated bacterial community belong to family GH11, which consists exclusively of xylanases.
30504145	9	74	theme	wood-feeding	1890:1901	arg1	termites					1910:1917	wood-feeding higher termites	1890:1917	wood-feeding higher termites	1890:1917	The major role of spirochetes in xylan degradation is unprecedented and assigns the fiber-associated Treponema clades in the hindgut of wood-feeding higher termites a prominent part in the breakdown of hemicelluloses.
30504145	2	75	theme	bacterial	378:386	arg1	microbiota					392:401	the bacterial gut microbiota	374:401	the bacterial gut microbiota	374:401	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	5	76	theme	major	889:893	arg1	xylan					878:882	xylan	878:882	xylan	878:882	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	5	76	theme	major	889:893	arg1	component					895:903	the major component	885:903	the major component of hemicellulose	885:920	Here, we demonstrate that the degradation of xylan, the major component of hemicellulose, is restricted to the hindgut compartment, where it is preferentially hydrolyzed over cellulose.
30504145	1	77	theme	endogenous	249:258	arg1	cellulases					265:274	endogenous host cellulases	249:274	endogenous host cellulases secreted in the midgut	249:297	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	2	78	theme	lignocellulose	518:531	arg1	degradation					533:543	lignocellulose degradation	518:543	lignocellulose degradation	518:543	The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous.
30504145	1	79	theme	hindgut	338:344	arg1	compartment					346:356	the hindgut compartment	334:356	the hindgut compartment	334:356	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	8	80	theme	compositional	1528:1540	arg1	binning					1542:1548	compositional binning	1528:1548	compositional binning of metagenomics contigs	1528:1572	Although the most abundant transcripts of GH11 in Nasutitermes takasagoensis were phylogenetically placed among their homologs of Firmicutes, immunofluorescence microscopy, compositional binning of metagenomics contigs, and the genomic context of the homologs indicated that they are encoded by Spirochaetes and were most likely obtained by horizontal gene transfer among the intestinal microbiota.
30504145	1	81	theme	wood-feeding	164:175	arg1	Termitidae					201:210	family Termitidae	194:210	family Termitidae	194:210	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	81	theme	wood-feeding	164:175	arg1	termites					184:191	wood-feeding higher termites	164:191	wood-feeding higher termites (family Termitidae)	164:211	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30504145	1	82	theme	host	260:263	arg1	cellulases					265:274	endogenous host cellulases	249:274	endogenous host cellulases secreted in the midgut	249:297	Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment.
30852274	5	0	theme	N-triethyl	1075:1084	arg1	chitosan					1086:1093	N-triethyl chitosan	1075:1093	N-triethyl chitosan	1075:1093	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	0	theme	N-triethyl	1075:1084	arg1	N					1071:1071	likely N	1064:1071	likely N	1064:1071	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	6	1	theme	own	1252:1254	arg1	biocomposites					1256:1268	our own biocomposites	1248:1268	our own biocomposites	1248:1268	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	5	2	theme	likely	1064:1069	arg1	N					1073:1073	N	1073:1073	N	1073:1073	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	2	theme	likely	1064:1069	arg1	chitosan					1086:1093	N-triethyl chitosan	1075:1093	N-triethyl chitosan	1075:1093	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	2	theme	likely	1064:1069	arg1	N					1071:1071	likely N	1064:1071	likely N	1064:1071	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	6	3	theme	N-triethyl	1275:1284	arg1	N					1271:1271	N	1271:1271	N	1271:1271	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	6	3	theme	N-triethyl	1275:1284	arg1	chitosan					1286:1293	N-triethyl chitosan	1275:1293	N-triethyl chitosan	1275:1293	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	4	4	theme	Coated	672:677	arg1	composites					679:688	Coated composites	672:688	Coated composites with N,N,N-triethyl chitosan	672:717	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	5	5	theme	other	1030:1034	arg1	biocomposites					1036:1048	other biocomposites	1030:1048	other biocomposites	1030:1048	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	0	6	theme	antibacterial	110:122	arg1	drugs					124:128	antibacterial drugs	110:128	antibacterial drugs	110:128	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	5	7	theme	CMC2/TEC	959:966	arg1	biocomposites					929:941	two biocomposites	925:941	two biocomposites of CMC1/TEC and CMC2/TEC	925:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	7	theme	CMC2/TEC	959:966	arg1	CMC2/TEC					959:966	CMC2/TEC	959:966	CMC2/TEC	959:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	7	theme	CMC2/TEC	959:966	arg1	CMC1/TEC					946:953	CMC1/TEC	946:953	CMC1/TEC	946:953	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	6	8	from	researches	1177:1186	arg1	strains					1207:1213	other bacterial strains	1191:1213	other bacterial strains	1191:1213	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	4	9	with	composites	679:688	arg1	chitosan					710:717	N-triethyl chitosan	699:717	N-triethyl chitosan	699:717	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	9	with	composites	679:688	arg1	N					695:695	N	695:695	N	695:695	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	9	with	composites	679:688	arg1	N					697:697	N	697:697	N	697:697	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	1	10	from	layers	321:326	arg1	pHs					345:347	two different pHs	331:347	two different pHs	331:347	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	5	11	theme	biocomposites	929:941	arg1	activities					911:920	antibacterial activities	897:920	antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC	897:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	4	12	from	biocomposites	811:823	arg1	release					786:792	the release	782:792	the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan	782:885	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	12	from	biocomposites	811:823	arg1	comparison					828:837	comparison	828:837	comparison with composites without N,N,N-triethyl chitosan	828:885	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	6	13	theme	advanced	1230:1237	arg1	tests					1239:1243	advanced tests	1230:1243	advanced tests on our own biocomposites	1230:1268	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	4	14	theme	drug	747:750	arg1	encapsulation					752:764	the drug encapsulation	743:764	the drug encapsulation	743:764	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	3	15	dep	DSC	564:566	arg1	BET					559:561	TG, SEM, BET, DSC, and disk diffusion method	550:593	BET	559:561	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	3	15	dep	DSC	564:566	arg1	SEM					554:556	TG, SEM, BET, DSC, and disk diffusion method	550:593	SEM	554:556	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	0	16	theme	drugs	124:128	arg1	consumption					95:105	consumption	95:105	consumption of antibacterial drugs	95:128	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	5	17	with	Study	888:892	arg1	rates					987:991	lower release rates	973:991	lower release rates	973:991	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	17	with	Study	888:892	arg1	concentrations					1003:1016	lower concentrations	997:1016	lower concentrations of CIP	997:1023	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	18	theme	CMC1/TEC	946:953	arg1	biocomposites					929:941	two biocomposites	925:941	two biocomposites of CMC1/TEC and CMC2/TEC	925:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	18	theme	CMC1/TEC	946:953	arg1	CMC2/TEC					959:966	CMC2/TEC	959:966	CMC2/TEC	959:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	18	theme	CMC1/TEC	946:953	arg1	CMC1/TEC					946:953	CMC1/TEC	946:953	CMC1/TEC	946:953	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	0	19	theme	natural	10:16	arg1	biocomposites					18:30	natural biocomposites	10:30	natural biocomposites of N-quaternary chitosan with antibacterial activity	10:83	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	0	19	theme	natural	10:16	arg1	chitosan					48:55	N-quaternary chitosan	35:55	N-quaternary chitosan	35:55	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	4	20	theme	N-triethyl	699:708	arg1	chitosan					710:717	N-triethyl chitosan	699:717	N-triethyl chitosan	699:717	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	20	theme	N-triethyl	699:708	arg1	N					695:695	N	695:695	N	695:695	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	1	21	theme	ciprofloxacin-	182:195	arg1	N					215:215	N	215:215	N	215:215	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	21	theme	ciprofloxacin-	182:195	arg1	chitosan					228:235	N-triethyl chitosan	217:235	N-triethyl chitosan (CMC/TEC and CMC׳/TEC)	217:258	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	21	theme	ciprofloxacin-	182:195	arg1	montmorillonite/N					197:213	ciprofloxacin- montmorillonite/N	182:213	ciprofloxacin- montmorillonite/N	182:213	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	5	22	theme	antibacterial	897:909	arg1	activities					911:920	antibacterial activities	897:920	antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC	897:966	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	1	23	theme	montmorillonite/N	197:213	arg1	biocomposites					165:177	biocomposites	165:177	biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC)	165:258	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	23	theme	montmorillonite/N	197:213	arg1	montmorillonite/N					197:213	ciprofloxacin- montmorillonite/N	182:213	ciprofloxacin- montmorillonite/N	182:213	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	4	24	contain	possessed	719:727	arg1	composites					679:688	Coated composites	672:688	Coated composites with N,N,N-triethyl chitosan	672:717	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	24	contain	possessed	719:727	arg2	efficiency					729:738	efficiency	729:738	efficiency of the drug encapsulation	729:764	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	6	25	dep	a	1346:1346	arg1	substitute					1348:1357	substitute	1348:1357	substitute in biomedical activities	1348:1382	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	3	26	theme	TG	550:551	arg1	DSC					564:566	TG, SEM, BET, DSC, and disk diffusion method	550:593	DSC	564:566	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	6	27	theme	bacterial	1197:1205	arg1	strains					1207:1213	other bacterial strains	1191:1213	other bacterial strains	1191:1213	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	6	28	theme	biomedical	1362:1371	arg1	activities					1373:1382	biomedical activities	1362:1382	biomedical activities	1362:1382	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	4	29	theme	N-triethyl	867:876	arg1	chitosan					878:885	N-triethyl chitosan	867:885	N-triethyl chitosan	867:885	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	4	29	theme	N-triethyl	867:876	arg1	N					863:863	N	863:863	N	863:863	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	3	30	theme	antibacterial	610:622	arg1	activity					624:631	antibacterial activity	610:631	antibacterial activity against Staphylococcus aureus	610:661	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	2	31	theme	FT-IR	398:402	arg1	data					404:407	XRD and FT-IR data	390:407	XRD and FT-IR data	390:407	XRD and FT-IR data demonstrated that ciprofloxacin (CIP) diffused among the layers of montmorillonite.
30852274	0	32	theme	chitosan	48:55	arg1	biocomposites					18:30	natural biocomposites	10:30	natural biocomposites of N-quaternary chitosan with antibacterial activity	10:83	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	0	32	theme	chitosan	48:55	arg1	chitosan					48:55	N-quaternary chitosan	35:55	N-quaternary chitosan	35:55	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	5	33	theme	ciprofloxacin	1148:1160	arg1	consumption					1133:1143	the consumption	1129:1143	the consumption of ciprofloxacin	1129:1160	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	1	34	theme	N-triethyl	369:378	arg1	chitosan					380:387	N-triethyl chitosan	369:387	N-triethyl chitosan	369:387	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	34	theme	N-triethyl	369:378	arg1	N					365:365	N	365:365	N	365:365	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	5	35	theme	release	979:985	arg1	rates					987:991	lower release rates	973:991	lower release rates	973:991	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	0	36	theme	N-quaternary	35:46	arg1	chitosan					48:55	N-quaternary chitosan	35:55	N-quaternary chitosan	35:55	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	1	37	theme	Na+-montmorillonite	301:319	arg1	layers					321:326	the Na+-montmorillonite layers	297:326	the Na+-montmorillonite layers in two different pHs	297:347	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	38	dep	chitosan	228:235	arg1	CMC/TEC					238:244	CMC/TEC	238:244	CMC/TEC	238:244	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	38	dep	chitosan	228:235	arg1	CMC׳/TEC					250:257	CMC׳/TEC	250:257	CMC׳/TEC	250:257	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	6	39	theme	other	1191:1195	arg1	strains					1207:1213	other bacterial strains	1191:1213	other bacterial strains	1191:1213	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	1	40	theme	N-triethyl	217:226	arg1	chitosan					228:235	N-triethyl chitosan	217:235	N-triethyl chitosan (CMC/TEC and CMC׳/TEC)	217:258	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	1	40	theme	N-triethyl	217:226	arg1	montmorillonite/N					197:213	ciprofloxacin- montmorillonite/N	182:213	ciprofloxacin- montmorillonite/N	182:213	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	0	41	theme	antibacterial	62:74	arg1	activity					76:83	antibacterial activity	62:83	antibacterial activity	62:83	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	4	42	theme	drug	797:800	arg1	release					786:792	the release	782:792	the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan	782:885	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	0	43	with	biocomposites	18:30	arg1	activity					76:83	antibacterial activity	62:83	antibacterial activity	62:83	Preparing natural biocomposites of N-quaternary chitosan with antibacterial activity to reduce consumption of antibacterial drugs.
30852274	5	44	theme	lower	997:1001	arg1	concentrations					1003:1016	lower concentrations	997:1016	lower concentrations of CIP	997:1023	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	45	theme	lower	973:977	arg1	rates					987:991	lower release rates	973:991	lower release rates	973:991	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	6	46	theme	more	1172:1175	arg1	researches					1177:1186	more researches	1172:1186	more researches on other bacterial strains	1172:1213	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	3	47	theme	diffusion	578:586	arg1	method					588:593	TG, SEM, BET, DSC, and disk diffusion method	550:593	method	588:593	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	5	48	theme	activities	911:920	arg1	Study					888:892	Study	888:892	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites	888:1048	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	49	theme	CIP	1021:1023	arg1	rates					987:991	lower release rates	973:991	lower release rates	973:991	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	5	49	theme	CIP	1021:1023	arg1	concentrations					1003:1016	lower concentrations	997:1016	lower concentrations of CIP	997:1023	Study of antibacterial activities of two biocomposites of CMC1/TEC and CMC2/TEC with lower release rates and lower concentrations of CIP than other biocomposites revealed that likely N,N,N-triethyl chitosan could considerably help to reduce the consumption of ciprofloxacin.
30852274	4	50	theme	encapsulation	752:764	arg1	efficiency					729:738	efficiency	729:738	efficiency of the drug encapsulation	729:764	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	6	51	from	tests	1239:1243	arg1	biocomposites					1256:1268	our own biocomposites	1248:1268	our own biocomposites	1248:1268	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	2	52	theme	montmorillonite	476:490	arg1	layers					466:471	the layers	462:471	the layers of montmorillonite	462:490	XRD and FT-IR data demonstrated that ciprofloxacin (CIP) diffused among the layers of montmorillonite.
30852274	2	53	theme	XRD	390:392	arg1	data					404:407	XRD and FT-IR data	390:407	XRD and FT-IR data	390:407	XRD and FT-IR data demonstrated that ciprofloxacin (CIP) diffused among the layers of montmorillonite.
30852274	3	54	theme	prepared	497:504	arg1	biocomposites					506:518	The prepared biocomposites	493:518	The prepared biocomposites	493:518	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
30852274	4	55	with	comparison	828:837	arg1	composites					844:853	composites	844:853	composites without N,N,N-triethyl chitosan	844:885	Coated composites with N,N,N-triethyl chitosan possessed efficiency of the drug encapsulation, and controlled the release of drug from the biocomposites in comparison with composites without N,N,N-triethyl chitosan.
30852274	6	56	theme	suitable	1323:1330	arg1	candidate					1332:1340	a suitable candidate	1321:1340	a suitable candidate for a substitute in biomedical activities	1321:1382	By doing more researches on other bacterial strains and performing advanced tests on our own biocomposites, N,N,N-triethyl chitosan can probably be proved as a suitable candidate for a substitute in biomedical activities.
30852274	1	57	theme	different	335:343	arg1	pHs					345:347	two different pHs	331:347	two different pHs	331:347	In this work, in order to prepare biocomposites of ciprofloxacin- montmorillonite/N,N,N-triethyl chitosan (CMC/TEC and CMC׳/TEC), ciprofloxacin was intercalated into the Na+-montmorillonite layers in two different pHs and coated with N,N,N-triethyl chitosan.
30852274	3	58	theme	disk	573:576	arg1	method					588:593	TG, SEM, BET, DSC, and disk diffusion method	550:593	method	588:593	The prepared biocomposites were further characterised by TG, SEM, BET, DSC, and disk diffusion method to investigate antibacterial activity against Staphylococcus aureus as well.
29620251	9	0	theme	observed	1549:1556	arg1	contrast					1565:1572	the observed SE-HIM contrast	1545:1572	the observed SE-HIM contrast	1545:1572	A charge-coupled mechanism explaining the observed SE-HIM contrast is proposed.
29620251	6	1	theme	minimum	969:975	arg1	gap					977:979	the minimum gap	965:979	the minimum gap measured between gold particles	965:1011	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	10	2	theme	oxide	1642:1646	arg1	particles					1648:1656	zinc oxide particles	1637:1656	zinc oxide particles on cells	1637:1665	Ionoluminescence-HIM was also performed targeting zinc oxide particles on cells.
29620251	7	3	from	diameter	1133:1140	arg1	~50-200 nm					1119:1128	~50-200 nm	1119:1128	~50-200 nm	1119:1128	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	5	4	theme	Gold-tagged	759:769	arg1	antibodies					771:780	Gold-tagged antibodies	759:780	Gold-tagged antibodies	759:780	Gold-tagged antibodies were employed to aid organelle identification.
29620251	2	5	theme	sample	248:253	arg1	region					255:260	only a small sample region	235:260	only a small sample region to emit secondary electrons (SEs)	235:294	Because the radiation induces only a small sample region to emit secondary electrons (SEs), very high resolution is expected.
29620251	1	6	theme	short	161:165	arg1	wavelength					178:187	the very short de Broglie wavelength	152:187	the very short de Broglie wavelength of helium ions	152:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	1	7	theme	Broglie	170:176	arg1	wavelength					178:187	the very short de Broglie wavelength	152:187	the very short de Broglie wavelength of helium ions	152:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	7	8	from	clusters	1092:1099	arg1	cells					1150:1154	COS7 cells	1145:1154	COS7 cells	1145:1154	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	7	9	theme	structures	1258:1267	arg1	formation					1231:1239	the formation	1227:1239	the formation of membrane raft structures that include membrane proteins	1227:1298	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	4	10	theme	high	589:592	arg1	images					609:614	high depth-of-field images	589:614	high depth-of-field images	589:614	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	7	11	theme	membrane	1244:1251	arg1	structures					1258:1267	membrane raft structures	1244:1267	membrane raft structures that include membrane proteins	1244:1298	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	11	12	dep	depth-of-field	1692:1705	arg1	high-resolution					1708:1722	high-resolution	1708:1722	high-resolution	1708:1722	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	8	13	theme	sections	1373:1380	arg1	images					1337:1342	high contrast images	1323:1342	high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1323:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	7	14	theme	~50-200 nm	1119:1128	arg1	particles					1109:1117	gold particles	1104:1117	gold particles ~50-200 nm in diameter	1104:1140	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	8	15	theme	Epon‑embedded	1385:1397	arg1	tissues					1412:1418	Epon‑embedded mouse kidney tissues	1385:1418	Epon‑embedded mouse kidney tissues	1385:1418	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	11	16	theme	depth-of-field	1692:1705	arg1	imaging					1724:1730	the high depth-of-field, high-resolution imaging	1683:1730	the high depth-of-field, high-resolution imaging achieved using HIM	1683:1749	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	0	17	theme	ion	62:64	arg1	microscopy					66:75	helium ion microscopy	55:75	helium ion microscopy	55:75	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	1	18	theme	fine	127:130	arg1	beam					136:139	a fine ion beam	125:139	a fine ion beam exploiting the very short de Broglie wavelength of helium ions	125:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	0	19	from	Imaging	0:6	arg1	cells					34:38	cells	34:38	cells	34:38	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	0	19	from	Imaging	0:6	arg1	tissues					44:50	tissues	44:50	tissues	44:50	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	4	20	from	support	750:756	arg1	background					730:739	a bright background	721:739	a bright background from the support	721:756	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	6	21	theme	secondary	893:901	arg1	SE					913:914	SE	913:914	SE	913:914	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	6	21	theme	secondary	893:901	arg1	electron 					903:911	secondary electron 	893:911	secondary electron (SE)-HIM	893:919	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	0	22	theme	helium	55:60	arg1	microscopy					66:75	helium ion microscopy	55:75	helium ion microscopy	55:75	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	7	23	theme	germ	1053:1056	arg1	labeling					1074:1081	Wheat germ agglutinin-gold labeling	1047:1081	Wheat germ agglutinin-gold labeling	1047:1081	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	7	24	from	~50-200 nm	1119:1128	arg1	diameter					1133:1140	diameter	1133:1140	diameter	1133:1140	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	1	25	theme	Helium	78:83	arg1	HIM					101:103	HIM	101:103	HIM	101:103	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	1	25	theme	Helium	78:83	arg1	microscopy					89:98	Helium ion microscopy	78:98	Helium ion microscopy (HIM) scans	78:110	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	6	26	theme	SiN	951:953	arg1	film					955:958	thin SiN film	946:958	thin SiN film	946:958	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	0	27	theme	immunogold	11:20	arg1	labeling					22:29	immunogold labeling	11:29	immunogold labeling	11:29	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	1	28	theme	microscopy	89:98	arg1	scans					106:110	Helium ion microscopy (HIM) scans	78:110	Helium ion microscopy (HIM) scans	78:110	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	7	29	theme	glycosylated	1193:1204	arg1	proteins					1206:1213	glycosylated proteins	1193:1213	glycosylated proteins	1193:1213	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	4	30	theme	bright	723:728	arg1	background					730:739	a bright background	721:739	a bright background from the support	721:756	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	10	31	theme	zinc	1637:1640	arg1	particles					1648:1656	zinc oxide particles	1637:1656	zinc oxide particles on cells	1637:1665	Ionoluminescence-HIM was also performed targeting zinc oxide particles on cells.
29620251	8	32	dep	unstained	1347:1355	arg1	uncoated					1358:1365	uncoated	1358:1365	uncoated	1358:1365	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	8	33	theme	sub-tissues	1474:1484	arg1	details					1463:1469	the details	1459:1469	the details of sub-tissues and cell organelles	1459:1504	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	8	34	theme	high	1323:1326	arg1	images					1337:1342	high contrast images	1323:1342	high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1323:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	3	35	theme	kidney	395:400	arg1	cells					413:417	COS7 kidney fibroblast cells	390:417	COS7 kidney fibroblast cells	390:417	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	8	36	theme	cell	1490:1493	arg1	organelles					1495:1504	cell organelles	1490:1504	cell organelles	1490:1504	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	4	37	theme	putative	693:700	arg1	mitochondria					702:713	putative mitochondria	693:713	putative mitochondria	693:713	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	3	38	from	applications	355:366	arg1	biology					381:387	biology	381:387	biology	381:387	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	2	39	theme	high	302:305	arg1	resolution					307:316	very high resolution	297:316	very high resolution	297:316	Because the radiation induces only a small sample region to emit secondary electrons (SEs), very high resolution is expected.
29620251	1	40	theme	helium	192:197	arg1	ions					199:202	helium ions	192:202	helium ions	192:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	7	41	theme	COS7	1145:1148	arg1	cells					1150:1154	COS7 cells	1145:1154	COS7 cells	1145:1154	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	3	42	theme	C2C12	423:427	arg1	cells					438:442	C2C12 myoblast cells	423:442	C2C12 myoblast cells	423:442	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	9	43	theme	SE-HIM	1558:1563	arg1	contrast					1565:1572	the observed SE-HIM contrast	1545:1572	the observed SE-HIM contrast	1545:1572	A charge-coupled mechanism explaining the observed SE-HIM contrast is proposed.
29620251	2	44	theme	small	242:246	arg1	region					255:260	only a small sample region	235:260	only a small sample region to emit secondary electrons (SEs)	235:294	Because the radiation induces only a small sample region to emit secondary electrons (SEs), very high resolution is expected.
29620251	11	45	theme	various	1776:1782	arg1	fields					1784:1789	various fields	1776:1789	various fields	1776:1789	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	11	45	theme	various	1776:1782	arg1	materials					1807:1815	soft materials	1802:1815	soft materials	1802:1815	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	1	46	theme	ion	132:134	arg1	beam					136:139	a fine ion beam	125:139	a fine ion beam exploiting the very short de Broglie wavelength of helium ions	125:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	10	47	from	particles	1648:1656	arg1	cells					1661:1665	cells	1661:1665	cells	1661:1665	Ionoluminescence-HIM was also performed targeting zinc oxide particles on cells.
29620251	7	48	theme	gold	1104:1107	arg1	particles					1109:1117	gold particles	1104:1117	gold particles ~50-200 nm in diameter	1104:1140	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	3	49	theme	nitride 	471:478	arg1	bilayer					488:494	a silicon (Si) nitride (SiN)/Si bilayer	456:494	a silicon (Si) nitride (SiN)/Si bilayer	456:494	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	8	50	theme	unstained	1347:1355	arg1	sections					1373:1380	unstained, uncoated, thin sections	1347:1380	unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1347:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	5	51	theme	organelle	803:811	arg1	identification					813:826	organelle identification	803:826	organelle identification	803:826	Gold-tagged antibodies were employed to aid organelle identification.
29620251	3	52	theme	/Si	484:486	arg1	bilayer					488:494	a silicon (Si) nitride (SiN)/Si bilayer	456:494	a silicon (Si) nitride (SiN)/Si bilayer	456:494	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	1	53	theme	de	167:168	arg1	wavelength					178:187	the very short de Broglie wavelength	152:187	the very short de Broglie wavelength of helium ions	152:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	7	54	theme	membrane	1282:1289	arg1	proteins					1291:1298	membrane proteins	1282:1298	membrane proteins	1282:1298	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	8	55	theme	kidney	1405:1410	arg1	tissues					1412:1418	Epon‑embedded mouse kidney tissues	1385:1418	Epon‑embedded mouse kidney tissues	1385:1418	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	6	56	theme	gold	846:849	arg1	tags					851:854	the gold tags	842:854	the gold tags	842:854	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	8	57	theme	thin	1368:1371	arg1	sections					1373:1380	unstained, uncoated, thin sections	1347:1380	unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1347:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	7	58	theme	raft	1253:1256	arg1	structures					1258:1267	membrane raft structures	1244:1267	membrane raft structures that include membrane proteins	1244:1298	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	4	59	theme	depth-of-field	594:607	arg1	images					609:614	high depth-of-field images	589:614	high depth-of-field images	589:614	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	7	60	theme	particles	1109:1117	arg1	clusters					1092:1099	clusters	1092:1099	clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins	1092:1213	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	8	61	theme	mouse	1399:1403	arg1	tissues					1412:1418	Epon‑embedded mouse kidney tissues	1385:1418	Epon‑embedded mouse kidney tissues	1385:1418	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	11	62	theme	soft	1802:1805	arg1	materials					1807:1815	soft materials	1802:1815	soft materials	1802:1815	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	7	63	gly	glycosylated	1193:1204	arg1	proteins					1206:1213	glycosylated proteins	1193:1213	glycosylated proteins	1193:1213	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	3	64	dep	nitride 	471:478	arg1	SiN					480:482	SiN	480:482	SiN	480:482	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	8	65	theme	tissues	1412:1418	arg1	sections					1373:1380	unstained, uncoated, thin sections	1347:1380	unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1347:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	4	66	theme	High	574:577	arg1	contrast					579:586	High contrast	574:586	High contrast	574:586	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	1	67	with	samples	112:118	arg1	beam					136:139	a fine ion beam	125:139	a fine ion beam exploiting the very short de Broglie wavelength of helium ions	125:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	6	68	theme	electron 	903:911	arg1	-HIM					916:919	secondary electron (SE)-HIM	893:919	secondary electron (SE)-HIM	893:919	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	2	69	theme	secondary	270:278	arg1	SEs					291:293	SEs	291:293	SEs	291:293	Because the radiation induces only a small sample region to emit secondary electrons (SEs), very high resolution is expected.
29620251	2	69	theme	secondary	270:278	arg1	electrons					280:288	secondary electrons	270:288	secondary electrons (SEs)	270:294	Because the radiation induces only a small sample region to emit secondary electrons (SEs), very high resolution is expected.
29620251	7	70	theme	agglutinin-gold	1058:1072	arg1	labeling					1074:1081	Wheat germ agglutinin-gold labeling	1047:1081	Wheat germ agglutinin-gold labeling	1047:1081	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	11	71	theme	high	1687:1690	arg1	imaging					1724:1730	the high depth-of-field, high-resolution imaging	1683:1730	the high depth-of-field, high-resolution imaging achieved using HIM	1683:1749	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	8	72	theme	SiN/Si	1433:1438	arg1	bilayer					1440:1446	a SiN/Si bilayer	1431:1446	a SiN/Si bilayer	1431:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	4	73	theme	endoplasmic	653:663	arg1	reticulum					665:673	endoplasmic reticulum	653:673	endoplasmic reticulum	653:673	High contrast, high depth-of-field images were obtained revealing the nucleus, endoplasmic reticulum, cytoskeleton and putative mitochondria above a bright background from the support.
29620251	3	74	theme	SE-HIM	371:376	arg1	applications					355:366	the applications	351:366	the applications of SE-HIM in biology	351:387	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	7	75	theme	Wheat	1047:1051	arg1	germ					1053:1056	Wheat germ	1047:1056	Wheat germ agglutinin-gold labeling	1047:1081	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	7	76	theme	proteins	1206:1213	arg1	assemblies					1179:1188	assemblies	1179:1188	assemblies of glycosylated proteins	1179:1213	Wheat germ agglutinin-gold labeling revealed clusters of gold particles ~50-200 nm in diameter on COS7 cells, which might represent assemblies of glycosylated proteins, suggesting the formation of membrane raft structures that include membrane proteins.
29620251	3	77	dep	explore	343:349	arg1	to					340:341	to	340:341	to	340:341	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	0	78	theme	labeling	22:29	arg1	Imaging					0:6	Imaging	0:6	Imaging of immunogold labeling in cells and tissues by helium ion microscopy	0:75	Imaging of immunogold labeling in cells and tissues by helium ion microscopy.
29620251	1	79	theme	ion	85:87	arg1	HIM					101:103	HIM	101:103	HIM	101:103	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	1	79	theme	ion	85:87	arg1	microscopy					89:98	Helium ion microscopy	78:98	Helium ion microscopy (HIM) scans	78:110	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29620251	6	80	theme	thin	946:949	arg1	film					955:958	thin SiN film	946:958	thin SiN film	946:958	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	3	81	theme	high	532:535	arg1	vacuum					537:542	high vacuum	532:542	high vacuum	532:542	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	3	82	theme	COS7	390:393	arg1	cells					413:417	COS7 kidney fibroblast cells	390:417	COS7 kidney fibroblast cells	390:417	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	3	83	theme	myoblast	429:436	arg1	cells					438:442	C2C12 myoblast cells	423:442	C2C12 myoblast cells	423:442	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	9	84	theme	charge-coupled	1509:1522	arg1	mechanism					1524:1532	A charge-coupled mechanism	1507:1532	A charge-coupled mechanism explaining the observed SE-HIM contrast	1507:1572	A charge-coupled mechanism explaining the observed SE-HIM contrast is proposed.
29620251	11	85	contain	have	1755:1758	arg2	applications					1760:1771	applications	1760:1771	applications	1760:1771	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	11	85	contain	have	1755:1758	arg1	imaging					1724:1730	the high depth-of-field, high-resolution imaging	1683:1730	the high depth-of-field, high-resolution imaging achieved using HIM	1683:1749	In conclusion, the high depth-of-field, high-resolution imaging achieved using HIM may have applications in various fields, including soft materials.
29620251	6	86	theme	gold	998:1001	arg1	particles					1003:1011	gold particles	998:1011	gold particles	998:1011	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	8	87	theme	organelles	1495:1504	arg1	details					1463:1469	the details	1459:1469	the details of sub-tissues and cell organelles	1459:1504	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	6	88	from	tags	851:854	arg1	Signals					829:835	Signals	829:835	Signals from the gold tags	829:854	Signals from the gold tags were most clearly distinguishable by secondary electron (SE)-HIM when cells were grown on thin SiN film, and the minimum gap measured between gold particles showed the resolution to be 2 nm.
29620251	3	89	theme	fibroblast	402:411	arg1	cells					413:417	COS7 kidney fibroblast cells	390:417	COS7 kidney fibroblast cells	390:417	In order to explore the applications of SE-HIM in biology, COS7 kidney fibroblast cells and C2C12 myoblast cells cultured on a silicon (Si) nitride (SiN)/Si bilayer were dried and directly observed in high vacuum, without coating or staining.
29620251	8	90	theme	contrast	1328:1335	arg1	images					1337:1342	high contrast images	1323:1342	high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer	1323:1446	SE-HIM also delivered high contrast images of unstained, uncoated, thin sections of Epon‑embedded mouse kidney tissues mounted on a SiN/Si bilayer, revealing the details of sub-tissues and cell organelles.
29620251	1	91	theme	ions	199:202	arg1	wavelength					178:187	the very short de Broglie wavelength	152:187	the very short de Broglie wavelength of helium ions	152:202	Helium ion microscopy (HIM) scans samples with a fine ion beam exploiting the very short de Broglie wavelength of helium ions.
29521393	8	0	theme	energy	1222:1227	arg1	metabolism					1229:1238	energy metabolism	1222:1238	energy metabolism	1222:1238	Furthermore, PP significantly increased amino acid metabolism, energy metabolism, and SCFA-related metabolism.
29521393	1	1	theme	initial	305:311	arg1	period					313:318	the initial period	301:318	the initial period after weaning	301:332	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	9	2	theme	function	1531:1538	arg1	maturation					1491:1500	the maturation	1487:1500	the maturation of gut microbiota biological function	1487:1538	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	2	3	from	effects	380:386	arg1	rats					454:457	weaned rats	447:457	weaned rats	447:457	In this study, we investigated the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats.
29521393	1	4	theme	important	136:144	arg1	Weaning					108:114	Weaning	108:114	Weaning	108:114	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	1	4	theme	important	136:144	arg1	event					146:150	an essential and important event	119:150	an essential and important event for infants and rodent animals	119:181	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	1	5	theme	crucial	224:230	arg1	role					232:235	a crucial role	222:235	a crucial role	222:235	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	0	6	from	effect	4:9	arg1	composition					67:77	gut microbiota composition	52:77	gut microbiota composition	52:77	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	0	6	from	effect	4:9	arg1	function					83:90	function	83:90	function	83:90	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	5	7	theme	body	776:779	arg1	weight					781:786	body weight	776:786	body weight	776:786	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	3	8	theme	Male	460:463	arg1	days					477:480	21 days	474:480	21 days of age	474:487	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	3	8	theme	Male	460:463	arg1	rats					468:471	Male SD rats	460:471	Male SD rats	460:471	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	9	9	theme	L.	1326:1327	arg1	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	9	theme	L.	1326:1327	arg1	NCFM					1341:1344	L. acidophilus NCFM	1326:1344	L. acidophilus NCFM	1326:1344	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	1	10	dep	composition	255:265	arg1	the					251:253	the	251:253	the	251:253	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	9	11	contain	containing	1303:1312	arg2	NCFM					1341:1344	L. acidophilus NCFM	1326:1344	L. acidophilus NCFM	1326:1344	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	11	contain	containing	1303:1312	arg2	Bi-07					1360:1364	B. lactis Bi-07	1350:1364	B. lactis Bi-07	1350:1364	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	11	contain	containing	1303:1312	arg2	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	11	contain	containing	1303:1312	arg1	synbiotics					1292:1301	synbiotics	1292:1301	synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan)	1292:1427	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	5	12	theme	barrier	829:835	arg1	integrity					837:845	gut epithelial barrier integrity	814:845	gut epithelial barrier integrity	814:845	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	9	13	theme	acidophilus	1329:1339	arg1	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	13	theme	acidophilus	1329:1339	arg1	NCFM					1341:1344	L. acidophilus NCFM	1326:1344	L. acidophilus NCFM	1326:1344	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	14	theme	growth	1557:1562	arg1	performance					1564:1574	the growth performance	1553:1574	the growth performance in weaned rats	1553:1589	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	15	theme	microbiota	1465:1474	arg1	composition					1446:1456	the composition	1442:1456	the composition of gut microbiota	1442:1474	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	7	16	theme	genus	987:991	arg1	level					993:997	the genus level	983:997	the genus level	983:997	At the genus level, Bifidobacterium, Lactobacillus, and Allobaculum were significantly increased, whereas Anaerostipes, Enterococcus, and Parabacteroides were observably reduced.
29521393	4	17	theme	Body	593:596	arg1	weight					598:603	Body weight	593:603	Body weight	593:603	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	8	18	theme	amino	1199:1203	arg1	metabolism					1210:1219	amino acid metabolism	1199:1219	amino acid metabolism	1199:1219	Furthermore, PP significantly increased amino acid metabolism, energy metabolism, and SCFA-related metabolism.
29521393	0	19	theme	rats	102:105	arg1	composition					67:77	gut microbiota composition	52:77	gut microbiota composition	52:77	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	0	19	theme	rats	102:105	arg1	function					83:90	function	83:90	function	83:90	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	4	20	theme	microbiota	721:730	arg1	function					642:649	gut epithelial barrier function	619:649	gut epithelial barrier function	619:649	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	4	20	theme	microbiota	721:730	arg1	intake					611:616	food intake	606:616	food intake	606:616	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	4	20	theme	microbiota	721:730	arg1	composition					689:699	composition	689:699	composition	689:699	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	4	20	theme	microbiota	721:730	arg1	function					705:712	function	705:712	function	705:712	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	4	20	theme	microbiota	721:730	arg1	activities					669:678	digestive enzyme activities	652:678	digestive enzyme activities	652:678	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	4	20	theme	microbiota	721:730	arg1	weight					598:603	Body weight	593:603	Body weight	593:603	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	6	21	theme	community	922:930	arg1	different					946:954	different	946:954	different	946:954	Moreover, the microbial community structure was different between the two groups.
29521393	6	21	theme	community	922:930	arg1	structure					932:940	the microbial community structure	908:940	the microbial community structure	908:940	Moreover, the microbial community structure was different between the two groups.
29521393	4	22	theme	enzyme	662:667	arg1	activities					669:678	digestive enzyme activities	652:678	digestive enzyme activities	652:678	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	5	23	theme	dietary	751:757	arg1	PP					759:760	The dietary PP	747:760	The dietary PP	747:760	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	0	24	theme	weaned	95:100	arg1	rats					102:105	weaned rats	95:105	weaned rats	95:105	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	1	25	from	function	271:278	arg1	period					313:318	the initial period	301:318	the initial period after weaning	301:332	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	4	26	theme	digestive	652:660	arg1	activities					669:678	digestive enzyme activities	652:678	digestive enzyme activities	652:678	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	9	27	theme	B.	1350:1351	arg1	Bi-07					1360:1364	B. lactis Bi-07	1350:1364	B. lactis Bi-07	1350:1364	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	27	theme	B.	1350:1351	arg1	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	4	28	theme	gut	619:621	arg1	function					642:649	gut epithelial barrier function	619:649	gut epithelial barrier function	619:649	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	3	29	dep	groups	512:517	arg1	group					524:528	one group	520:528	two groups: one group was administered with PP for four weeks	508:568	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	9	30	from	performance	1564:1574	arg1	rats					1586:1589	weaned rats	1579:1589	weaned rats	1579:1589	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	1	31	from	composition	255:265	arg1	period					313:318	the initial period	301:318	the initial period after weaning	301:332	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	0	32	dep	composition	67:77	arg1	the					48:50	the	48:50	the	48:50	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	1	33	theme	rodent	168:173	arg1	animals					175:181	rodent animals	168:181	rodent animals	168:181	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	2	34	theme	probiotics	395:404	arg1	effects					380:386	the potential effects	366:386	the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats	366:457	In this study, we investigated the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats.
29521393	9	35	theme	weaned	1579:1584	arg1	rats					1586:1589	weaned rats	1579:1589	weaned rats	1579:1589	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	3	36	theme	age	485:487	arg1	days					477:480	21 days	474:480	21 days of age	474:487	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	3	36	theme	age	485:487	arg1	rats					468:471	Male SD rats	460:471	Male SD rats	460:471	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	5	37	theme	gut	814:816	arg1	integrity					837:845	gut epithelial barrier integrity	814:845	gut epithelial barrier integrity	814:845	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	9	38	dep	polysaccharides	1387:1401	arg1	LBP					1404:1406	LBP	1404:1406	LBP	1404:1406	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	38	dep	polysaccharides	1387:1401	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides (LBP, PCPs, and Lentinan)	1387:1427	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	38	dep	polysaccharides	1387:1401	arg1	PCPs					1409:1412	PCPs	1409:1412	PCPs	1409:1412	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	38	dep	polysaccharides	1387:1401	arg1	Lentinan					1419:1426	Lentinan	1419:1426	Lentinan	1419:1426	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	4	39	theme	epithelial	623:632	arg1	function					642:649	gut epithelial barrier function	619:649	gut epithelial barrier function	619:649	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	3	40	theme	SD	465:466	arg1	days					477:480	21 days	474:480	21 days of age	474:487	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	3	40	theme	SD	465:466	arg1	rats					468:471	Male SD rats	460:471	Male SD rats	460:471	Male SD rats, 21 days of age, were divided into two groups: one group was administered with PP for four weeks and the other was not.
29521393	0	41	theme	polysaccharides	29:43	arg1	effect					4:9	The effect	0:9	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats	0:105	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	9	42	theme	gut	1461:1463	arg1	microbiota					1465:1474	gut microbiota	1461:1474	gut microbiota	1461:1474	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	6	43	theme	microbial	912:920	arg1	different					946:954	different	946:954	different	946:954	Moreover, the microbial community structure was different between the two groups.
29521393	6	43	theme	microbial	912:920	arg1	structure					932:940	the microbial community structure	908:940	the microbial community structure	908:940	Moreover, the microbial community structure was different between the two groups.
29521393	5	44	theme	enzymes	889:895	arg1	activities					865:874	the activities	861:874	the activities of digestive enzymes	861:895	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	2	45	theme	potential	370:378	arg1	effects					380:386	the potential effects	366:386	the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats	366:457	In this study, we investigated the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats.
29521393	0	46	theme	probiotics	14:23	arg1	effect					4:9	The effect	0:9	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats	0:105	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	4	47	dep	composition	689:699	arg1	the					685:687	the	685:687	the	685:687	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	1	48	theme	gut	283:285	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	7	49	dep	increased	1067:1075	arg1	whereas					1078:1084	whereas	1078:1084	whereas	1078:1084	At the genus level, Bifidobacterium, Lactobacillus, and Allobaculum were significantly increased, whereas Anaerostipes, Enterococcus, and Parabacteroides were observably reduced.
29521393	2	50	theme	weaned	447:452	arg1	rats					454:457	weaned rats	447:457	weaned rats	447:457	In this study, we investigated the potential effects of two probiotics along with three polysaccharides (PP) in weaned rats.
29521393	5	51	theme	epithelial	818:827	arg1	integrity					837:845	gut epithelial barrier integrity	814:845	gut epithelial barrier integrity	814:845	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	1	52	theme	microbiota	287:296	arg1	function					271:278	function	271:278	function	271:278	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	1	52	theme	microbiota	287:296	arg1	composition					255:265	composition	255:265	composition	255:265	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	9	53	with	combination	1370:1380	arg1	LBP					1404:1406	LBP	1404:1406	LBP	1404:1406	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	53	with	combination	1370:1380	arg1	polysaccharides					1387:1401	polysaccharides	1387:1401	polysaccharides (LBP, PCPs, and Lentinan)	1387:1427	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	53	with	combination	1370:1380	arg1	PCPs					1409:1412	PCPs	1409:1412	PCPs	1409:1412	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	53	with	combination	1370:1380	arg1	Lentinan					1419:1426	Lentinan	1419:1426	Lentinan	1419:1426	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	4	54	theme	gut	717:719	arg1	microbiota					721:730	gut microbiota	717:730	gut microbiota	717:730	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	5	55	theme	food	792:795	arg1	intake					797:802	food intake	792:802	food intake	792:802	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	9	56	theme	biological	1520:1529	arg1	function					1531:1538	gut microbiota biological function	1505:1538	gut microbiota biological function	1505:1538	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	8	57	theme	acid	1205:1208	arg1	metabolism					1210:1219	amino acid metabolism	1199:1219	amino acid metabolism	1199:1219	Furthermore, PP significantly increased amino acid metabolism, energy metabolism, and SCFA-related metabolism.
29521393	4	58	theme	barrier	634:640	arg1	function					642:649	gut epithelial barrier function	619:649	gut epithelial barrier function	619:649	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	1	59	theme	dietary	192:198	arg1	supplementation					200:214	the dietary supplementation	188:214	the dietary supplementation	188:214	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	8	60	theme	SCFA-related	1245:1256	arg1	metabolism					1258:1267	SCFA-related metabolism	1245:1267	SCFA-related metabolism	1245:1267	Furthermore, PP significantly increased amino acid metabolism, energy metabolism, and SCFA-related metabolism.
29521393	9	61	theme	gut	1505:1507	arg1	function					1531:1538	gut microbiota biological function	1505:1538	gut microbiota biological function	1505:1538	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	4	62	theme	food	606:609	arg1	intake					611:616	food intake	606:616	food intake	606:616	Body weight, food intake, gut epithelial barrier function, digestive enzyme activities, and the composition and function of gut microbiota were analyzed.
29521393	0	63	theme	microbiota	56:65	arg1	composition					67:77	gut microbiota composition	52:77	gut microbiota composition	52:77	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	9	64	theme	microbiota	1509:1518	arg1	function					1531:1538	gut microbiota biological function	1505:1538	gut microbiota biological function	1505:1538	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	1	65	theme	essential	122:130	arg1	Weaning					108:114	Weaning	108:114	Weaning	108:114	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	1	65	theme	essential	122:130	arg1	event					146:150	an essential and important event	119:150	an essential and important event for infants and rodent animals	119:181	Weaning is an essential and important event for infants and rodent animals, and the dietary supplementation plays a crucial role in regulating the composition and function of gut microbiota in the initial period after weaning.
29521393	0	66	theme	gut	52:54	arg1	composition					67:77	gut microbiota composition	52:77	gut microbiota composition	52:77	The effect of probiotics and polysaccharides on the gut microbiota composition and function of weaned rats.
29521393	9	67	theme	lactis	1353:1358	arg1	Bi-07					1360:1364	B. lactis Bi-07	1350:1364	B. lactis Bi-07	1350:1364	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	67	theme	lactis	1353:1358	arg1	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	5	68	theme	digestive	879:887	arg1	enzymes					889:895	digestive enzymes	879:895	digestive enzymes	879:895	The dietary PP increased the body weight and food intake, promoted gut epithelial barrier integrity, and elevated the activities of digestive enzymes.
29521393	9	69	dep	probiotics	1314:1323	arg1	Bi-07					1360:1364	B. lactis Bi-07	1350:1364	B. lactis Bi-07	1350:1364	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	69	dep	probiotics	1314:1323	arg1	probiotics					1314:1323	probiotics	1314:1323	probiotics (L. acidophilus NCFM and B. lactis Bi-07)	1314:1365	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
29521393	9	69	dep	probiotics	1314:1323	arg1	NCFM					1341:1344	L. acidophilus NCFM	1326:1344	L. acidophilus NCFM	1326:1344	This study shows that synbiotics containing probiotics (L. acidophilus NCFM and B. lactis Bi-07) in combination with polysaccharides (LBP, PCPs, and Lentinan) can modulate the composition of gut microbiota, stimulate the maturation of gut microbiota biological function, and promote the growth performance in weaned rats.
31542050	0	0	theme	hydrolysis	87:96	arg1	mechanism					60:68	the mechanism	56:68	the mechanism of hemicellulose hydrolysis	56:96	Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.
31542050	2	1	from	composition	436:446	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	1	from	composition	436:446	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	4	2	theme	GH10	697:700	arg1	family					702:707	the GH10 family	693:707	the GH10 family	693:707	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	2	3	from	mode	473:476	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	3	from	mode	473:476	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	1	4	theme	internal	171:178	arg1	bonds					197:201	the internal β-1,4-glycosidic bonds	167:201	the internal β-1,4-glycosidic bonds in the xylan backbone	167:223	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	12	5	theme	first	1829:1833	arg1	time					1835:1838	the first time	1825:1838	the first time	1825:1838	This study demonstrated, for the first time, that the hydrolysates of GH11 xylanases can be further hydrolyzed by GH10 xylanases, but not vice versa.
31542050	0	6	theme	hemicellulose	73:85	arg1	hydrolysis					87:96	hemicellulose hydrolysis	73:96	hemicellulose hydrolysis	73:96	Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.
31542050	2	7	from	effect	461:466	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	7	from	effect	461:466	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	1	8	theme	β-1,4-glycosidic	180:195	arg1	bonds					197:201	the internal β-1,4-glycosidic bonds	167:201	the internal β-1,4-glycosidic bonds in the xylan backbone	167:223	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	9	9	theme	long-chain	1422:1431	arg1	xylooligosaccharides					1433:1452	long-chain xylooligosaccharides	1422:1452	long-chain xylooligosaccharides	1422:1452	Xyl2 was shown to hydrolyze xylan to long-chain xylooligosaccharides, whereas Xyl3 hydrolyzed xylan to xylooligosaccharides with a lower degree of polymerization.
31542050	1	10	theme	carbohydrate-active	272:290	arg1	database					306:313	the carbohydrate-active enzyme (CAZy) database	268:313	the carbohydrate-active enzyme (CAZy) database	268:313	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	12	11	theme	GH10	1910:1913	arg1	xylanases					1915:1923	GH10 xylanases	1910:1923	GH10 xylanases	1910:1923	This study demonstrated, for the first time, that the hydrolysates of GH11 xylanases can be further hydrolyzed by GH10 xylanases, but not vice versa.
31542050	10	12	from	families	1664:1671	arg1	xylanases					1639:1647	xylanases	1639:1647	xylanases from different families	1639:1671	CONCLUSIONS Synergistic effect exists among different xylanases, and it was higher between xylanases from different families.
31542050	1	13	theme	enzyme	292:297	arg1	database					306:313	the carbohydrate-active enzyme (CAZy) database	268:313	the carbohydrate-active enzyme (CAZy) database	268:313	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	2	14	theme	xylanases	509:517	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	14	theme	xylanases	509:517	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	15	theme	many	378:381	arg1	fungi					395:399	many filamentous fungi	378:399	many filamentous fungi	378:399	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	11	16	theme	observed	1750:1757	arg1	synergy					1759:1765	the observed synergy	1746:1765	the observed synergy between different xylanases	1746:1793	The cooperation of hydrolysis modes comprised the primary mechanism for the observed synergy between different xylanases.
31542050	4	17	contain	contains	718:725	arg1	C-terminal					748:757	C-terminal	748:757	C-terminal	748:757	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	4	17	contain	contains	718:725	arg2	Xyl3					713:716	Xyl3	713:716	Xyl3	713:716	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	4	17	contain	contains	718:725	arg1	Xyl3					713:716	Xyl3	713:716	Xyl3	713:716	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	4	17	contain	contains	718:725	arg2	domain					734:739	a CBM1 domain	727:739	a CBM1 domain	727:739	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	12	18	theme	xylanases	1871:1879	arg1	hydrolysates					1850:1861	the hydrolysates	1846:1861	the hydrolysates of GH11 xylanases	1846:1879	This study demonstrated, for the first time, that the hydrolysates of GH11 xylanases can be further hydrolyzed by GH10 xylanases, but not vice versa.
31542050	6	19	theme	different	1038:1046	arg1	families					1048:1055	different families	1038:1055	different families	1038:1055	The three xylanases exhibited synergistic effects, with the maximum synergy observed between Xyl3 and Xyl2, which are from different families.
31542050	0	20	theme	chrysogenum	113:123	arg1	P33					125:127	Penicillium chrysogenum P33	101:127	Penicillium chrysogenum P33	101:127	Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.
31542050	2	21	located	detected	348:355	arg1	strains					367:373	single strains	360:373	single strains of many filamentous fungi	360:399	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	21	located	detected	348:355	arg2	xylanases					334:342	multiple xylanases	325:342	multiple xylanases	325:342	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	12	22	theme	GH11	1866:1869	arg1	xylanases					1871:1879	GH11 xylanases	1866:1879	GH11 xylanases	1866:1879	This study demonstrated, for the first time, that the hydrolysates of GH11 xylanases can be further hydrolyzed by GH10 xylanases, but not vice versa.
31542050	4	23	dep	belong	683:688	arg1	whereas					760:766	whereas	760:766	whereas	760:766	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	2	24	theme	action	481:486	arg1	composition					436:446	composition	436:446	composition	436:446	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	24	theme	action	481:486	arg1	effect					461:466	synergistic effect	449:466	synergistic effect	449:466	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	24	theme	action	481:486	arg1	mode					473:476	mode	473:476	mode	473:476	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	6	25	theme	synergistic	945:955	arg1	effects					957:963	synergistic effects	945:963	synergistic effects	945:963	The three xylanases exhibited synergistic effects, with the maximum synergy observed between Xyl3 and Xyl2, which are from different families.
31542050	4	26	theme	CBM1	729:732	arg1	domain					734:739	a CBM1 domain	727:739	a CBM1 domain	727:739	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	10	27	theme	Synergistic	1560:1570	arg1	effect					1572:1577	CONCLUSIONS Synergistic effect	1548:1577	CONCLUSIONS Synergistic effect	1548:1577	CONCLUSIONS Synergistic effect exists among different xylanases, and it was higher between xylanases from different families.
31542050	2	28	theme	synergistic	449:459	arg1	effect					461:466	synergistic effect	449:466	synergistic effect	449:466	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	1	29	theme	CAZy	300:303	arg1	database					306:313	the carbohydrate-active enzyme (CAZy) database	268:313	the carbohydrate-active enzyme (CAZy) database	268:313	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	1	30	theme	xylan	210:214	arg1	backbone					216:223	the xylan backbone	206:223	the xylan backbone	206:223	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	0	31	theme	modes	26:30	arg1	Cooperation					0:10	Cooperation	0:10	Cooperation of hydrolysis modes among xylanases	0:46	Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.
31542050	2	32	theme	multiple	325:332	arg1	xylanases					334:342	multiple xylanases	325:342	multiple xylanases	325:342	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	5	33	theme	optimal	805:811	arg1	values					828:833	The optimal temperature/pH values	801:833	The optimal temperature/pH values	801:833	The optimal temperature/pH values were 35 °C/6.0, 50 °C/5.0 and 55 °C/6.0 for Xyl1, Xyl2, and Xyl3, respectively.
31542050	5	33	theme	optimal	805:811	arg1	35 °C/6.0					840:848	35 °C/6.0	840:848	35 °C/6.0	840:848	The optimal temperature/pH values were 35 °C/6.0, 50 °C/5.0 and 55 °C/6.0 for Xyl1, Xyl2, and Xyl3, respectively.
31542050	0	34	theme	hydrolysis	15:24	arg1	modes					26:30	hydrolysis modes	15:30	hydrolysis modes	15:30	Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.
31542050	11	35	theme	different	1775:1783	arg1	xylanases					1785:1793	different xylanases	1775:1793	different xylanases	1775:1793	The cooperation of hydrolysis modes comprised the primary mechanism for the observed synergy between different xylanases.
31542050	2	36	theme	same	535:538	arg1	microorganism					540:552	the same microorganism	531:552	the same microorganism	531:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	5	37	theme	temperature/pH	813:826	arg1	values					828:833	The optimal temperature/pH values	801:833	The optimal temperature/pH values	801:833	The optimal temperature/pH values were 35 °C/6.0, 50 °C/5.0 and 55 °C/6.0 for Xyl1, Xyl2, and Xyl3, respectively.
31542050	5	37	theme	temperature/pH	813:826	arg1	35 °C/6.0					840:848	35 °C/6.0	840:848	35 °C/6.0	840:848	The optimal temperature/pH values were 35 °C/6.0, 50 °C/5.0 and 55 °C/6.0 for Xyl1, Xyl2, and Xyl3, respectively.
31542050	2	38	theme	single	360:365	arg1	strains					367:373	single strains	360:373	single strains of many filamentous fungi	360:399	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	39	from	action	481:486	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	39	from	action	481:486	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	7	40	theme	C + Xyl2 + Xyl3	1230:1244	arg1	combination					1215:1225	the combination	1211:1225	the combination of C + Xyl2 + Xyl3	1211:1244	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	10	41	theme	CONCLUSIONS	1548:1558	arg1	effect					1572:1577	CONCLUSIONS Synergistic effect	1548:1577	CONCLUSIONS Synergistic effect	1548:1577	CONCLUSIONS Synergistic effect exists among different xylanases, and it was higher between xylanases from different families.
31542050	4	42	theme	GH11	788:791	arg1	family					793:798	the GH11 family	784:798	the GH11 family	784:798	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	10	43	theme	different	1592:1600	arg1	xylanases					1602:1610	different xylanases	1592:1610	different xylanases	1592:1610	CONCLUSIONS Synergistic effect exists among different xylanases, and it was higher between xylanases from different families.
31542050	1	44	from	families	256:263	arg1	database					306:313	the carbohydrate-active enzyme (CAZy) database	268:313	the carbohydrate-active enzyme (CAZy) database	268:313	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	8	45	theme	enzymatic	1260:1268	arg1	activity					1270:1277	the enzymatic activity	1256:1277	the enzymatic activity of Xyl1 toward xylan	1256:1298	Although the enzymatic activity of Xyl1 toward xylan was low, it was shown to be capable of hydrolyzing xylooligosaccharides into xylose.
31542050	8	45	theme	enzymatic	1260:1268	arg1	low					1304:1306	low	1304:1306	low	1304:1306	Although the enzymatic activity of Xyl1 toward xylan was low, it was shown to be capable of hydrolyzing xylooligosaccharides into xylose.
31542050	7	46	theme	reducing	1167:1174	arg1	5.68 mg/mL					1184:1193	5.68 mg/mL	1184:1193	5.68 mg/mL	1184:1193	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	7	46	theme	reducing	1167:1174	arg1	sugars					1176:1181	reducing sugars	1167:1181	reducing sugars (5.68 mg/mL)	1167:1194	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	11	47	theme	modes	1704:1708	arg1	cooperation					1678:1688	The cooperation	1674:1688	The cooperation of hydrolysis modes	1674:1708	The cooperation of hydrolysis modes comprised the primary mechanism for the observed synergy between different xylanases.
31542050	7	48	theme	maximum	1149:1155	arg1	5.68 mg/mL					1184:1193	5.68 mg/mL	1184:1193	5.68 mg/mL	1184:1193	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	7	48	theme	maximum	1149:1155	arg1	amount					1157:1162	the maximum amount	1145:1162	the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3	1145:1244	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	7	48	theme	maximum	1149:1155	arg1	sugars					1176:1181	reducing sugars	1167:1181	reducing sugars (5.68 mg/mL)	1167:1194	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	1	49	theme	BACKGROUND	130:139	arg1	Xylanases					141:149	BACKGROUND Xylanases	130:149	BACKGROUND Xylanases	130:149	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	7	50	theme	cellulase	1125:1133	arg1	hydrolysis					1111:1120	the hydrolysis	1107:1120	the hydrolysis of cellulase (C)	1107:1137	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	2	51	from	set	502:504	arg1	composition					436:446	composition	436:446	composition	436:446	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	51	from	set	502:504	arg1	effect					461:466	synergistic effect	449:466	synergistic effect	449:466	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	51	from	set	502:504	arg1	mode					473:476	mode	473:476	mode	473:476	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	9	52	dep	shown	1394:1398	arg1	whereas					1455:1461	whereas	1455:1461	whereas	1455:1461	Xyl2 was shown to hydrolyze xylan to long-chain xylooligosaccharides, whereas Xyl3 hydrolyzed xylan to xylooligosaccharides with a lower degree of polymerization.
31542050	9	53	theme	polymerization	1532:1545	arg1	degree					1522:1527	a lower degree	1514:1527	a lower degree of polymerization	1514:1545	Xyl2 was shown to hydrolyze xylan to long-chain xylooligosaccharides, whereas Xyl3 hydrolyzed xylan to xylooligosaccharides with a lower degree of polymerization.
31542050	9	54	theme	lower	1516:1520	arg1	degree					1522:1527	a lower degree	1514:1527	a lower degree of polymerization	1514:1545	Xyl2 was shown to hydrolyze xylan to long-chain xylooligosaccharides, whereas Xyl3 hydrolyzed xylan to xylooligosaccharides with a lower degree of polymerization.
31542050	11	55	theme	primary	1724:1730	arg1	mechanism					1732:1740	the primary mechanism	1720:1740	the primary mechanism for the observed synergy between different xylanases	1720:1793	The cooperation of hydrolysis modes comprised the primary mechanism for the observed synergy between different xylanases.
31542050	3	56	dep	RESULTS	555:561	arg1	xylanases					573:581	All three xylanases	563:581	RESULTS All three xylanases secreted by Penicillium chrysogenum P33	555:621	RESULTS All three xylanases secreted by Penicillium chrysogenum P33 were expressed and characterized.
31542050	8	57	theme	Xyl1	1282:1285	arg1	activity					1270:1277	the enzymatic activity	1256:1277	the enzymatic activity of Xyl1 toward xylan	1256:1298	Although the enzymatic activity of Xyl1 toward xylan was low, it was shown to be capable of hydrolyzing xylooligosaccharides into xylose.
31542050	8	57	theme	Xyl1	1282:1285	arg1	low					1304:1306	low	1304:1306	low	1304:1306	Although the enzymatic activity of Xyl1 toward xylan was low, it was shown to be capable of hydrolyzing xylooligosaccharides into xylose.
31542050	1	58	theme	different	246:254	arg1	families					256:263	different families	246:263	different families in the carbohydrate-active enzyme (CAZy) database	246:313	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	2	59	theme	complete	493:500	arg1	xylanases					509:517	xylanases	509:517	xylanases secreted by the same microorganism	509:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	2	59	theme	complete	493:500	arg1	set					502:504	a complete set	491:504	a complete set of xylanases secreted by the same microorganism	491:552	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	11	60	theme	hydrolysis	1693:1702	arg1	modes					1704:1708	hydrolysis modes	1693:1708	hydrolysis modes	1693:1708	The cooperation of hydrolysis modes comprised the primary mechanism for the observed synergy between different xylanases.
31542050	3	61	theme	chrysogenum	607:617	arg1	P33					619:621	Penicillium chrysogenum P33	595:621	Penicillium chrysogenum P33	595:621	RESULTS All three xylanases secreted by Penicillium chrysogenum P33 were expressed and characterized.
31542050	10	62	theme	different	1654:1662	arg1	families					1664:1671	different families	1654:1671	different families	1654:1671	CONCLUSIONS Synergistic effect exists among different xylanases, and it was higher between xylanases from different families.
31542050	2	63	theme	fungi	395:399	arg1	strains					367:373	single strains	360:373	single strains of many filamentous fungi	360:399	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	7	64	theme	sugars	1176:1181	arg1	5.68 mg/mL					1184:1193	5.68 mg/mL	1184:1193	5.68 mg/mL	1184:1193	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	7	64	theme	sugars	1176:1181	arg1	amount					1157:1162	the maximum amount	1145:1162	the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3	1145:1244	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	7	64	theme	sugars	1176:1181	arg1	sugars					1176:1181	reducing sugars	1167:1181	reducing sugars (5.68 mg/mL)	1167:1194	The synergy between xylanases could also improve the hydrolysis of cellulase (C), with the maximum amount of reducing sugars (5.68 mg/mL) observed using the combination of C + Xyl2 + Xyl3.
31542050	4	65	dep	enzymes	661:667	arg1	Xyl1					669:672	Xyl1	669:672	Xyl1	669:672	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	4	65	dep	enzymes	661:667	arg1	enzymes					661:667	The enzymes Xyl1 and Xyl3	657:681	The enzymes Xyl1 and Xyl3	657:681	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	4	65	dep	enzymes	661:667	arg1	Xyl3					678:681	Xyl3	678:681	Xyl3	678:681	The enzymes Xyl1 and Xyl3 belong to the GH10 family and Xyl3 contains a CBM1 domain at its C-terminal, whereas Xyl2 belongs to the GH11 family.
31542050	1	66	from	bonds	197:201	arg1	backbone					216:223	the xylan backbone	206:223	the xylan backbone	206:223	BACKGROUND Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database.
31542050	2	67	theme	filamentous	383:393	arg1	fungi					395:399	many filamentous fungi	378:399	many filamentous fungi	378:399	Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, synergistic effect, and mode of action in a complete set of xylanases secreted by the same microorganism.
31542050	6	68	theme	maximum	975:981	arg1	synergy					983:989	the maximum synergy	971:989	the maximum synergy observed between Xyl3 and Xyl2, which are from different families	971:1055	The three xylanases exhibited synergistic effects, with the maximum synergy observed between Xyl3 and Xyl2, which are from different families.
30776441	4	0	theme	hydrogels	776:784	arg1	strength					760:767	the strength	756:767	the strength of the hydrogels (up to 7.3 MPa)	756:800	The dual network structure significantly enhances the strength of the hydrogels (up to 7.3 MPa).
30776441	1	1	theme	network	173:179	arg1	hydrogels					212:220	dual network self-healing alginate/guar gum hydrogels	168:220	dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles	168:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	6	2	theme	materials	1091:1099	arg1	design					1043:1048	design	1043:1048	design	1043:1048	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	6	2	theme	materials	1091:1099	arg1	synthesis					1054:1062	synthesis	1054:1062	synthesis	1054:1062	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	0	3	theme	polydopamine-type	78:94	arg1	microcapsules					96:108	polydopamine-type microcapsules	78:108	polydopamine-type microcapsules from mesoporous silica nanoparticles	78:145	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	1	4	theme	self-healing	181:192	arg1	hydrogels					212:220	dual network self-healing alginate/guar gum hydrogels	168:220	dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles	168:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	6	5	theme	self-healing	1078:1089	arg1	materials					1091:1099	functional self-healing materials	1067:1099	functional self-healing materials	1067:1099	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	0	6	theme	mesoporous	115:124	arg1	nanoparticles					133:145	mesoporous silica nanoparticles	115:145	mesoporous silica nanoparticles	115:145	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	6	7	theme	self-healing	974:985	arg1	hydrogels					987:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	4	8	dep	hydrogels	776:784	arg1	up					787:788	up	787:788	up to 7.3 MPa	787:799	The dual network structure significantly enhances the strength of the hydrogels (up to 7.3 MPa).
30776441	1	9	theme	one-pot	327:333	arg1	method					335:340	one-pot method	327:340	one-pot method	327:340	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	6	10	theme	obtained	965:972	arg1	hydrogels					987:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	2	11	theme	guar	409:412	arg1	gum					414:416	guar gum	409:416	guar gum	409:416	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	5	12	theme	self-healing	861:872	arg1	performance					874:884	excellent self-healing performance	851:884	excellent self-healing performance	851:884	It has also proved that these hydrogels exhibit excellent self-healing performance at ambient temperatures, which self-healing efficiency can reach to 95.5%.
30776441	6	13	contain	have	997:1000	arg1	hydrogels					987:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels	961:995	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	6	13	contain	have	997:1000	arg2	prospect					1026:1033	a promising application prospect	1002:1033	a promising application prospect for the design and synthesis of functional self-healing materials	1002:1099	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	1	14	theme	alginate/guar	194:206	arg1	hydrogels					212:220	dual network self-healing alginate/guar gum hydrogels	168:220	dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles	168:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	0	15	theme	silica	126:131	arg1	nanoparticles					133:145	mesoporous silica nanoparticles	115:145	mesoporous silica nanoparticles	115:145	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	1	16	theme	gum	208:210	arg1	hydrogels					212:220	dual network self-healing alginate/guar gum hydrogels	168:220	dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles	168:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	4	17	theme	dual	710:713	arg1	structure					723:731	The dual network structure	706:731	The dual network structure	706:731	The dual network structure significantly enhances the strength of the hydrogels (up to 7.3 MPa).
30776441	3	18	theme	Metal-ligand	453:464	arg1	interactions					466:477	Metal-ligand interactions	453:477	Metal-ligand interactions as reversible non-covalent bonds	453:510	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	3	19	theme	reversible	482:491	arg1	bonds					506:510	reversible non-covalent bonds	482:510	reversible non-covalent bonds	482:510	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	6	20	dep	design	1043:1048	arg1	the					1039:1041	the	1039:1041	the	1039:1041	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	2	21	theme	sodium	422:427	arg1	alginate					429:436	sodium alginate	422:436	sodium alginate	422:436	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	0	22	theme	network	20:26	arg1	gum					55:57	dual network self-healing alginate/guar gum	15:57	dual network self-healing alginate/guar gum	15:57	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	3	23	dep	reversible	482:491	arg1	non-covalent					493:504	non-covalent	493:504	non-covalent	493:504	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	1	24	theme	polydopamine-type	227:243	arg1	microcapsules					245:257	polydopamine-type microcapsules	227:257	polydopamine-type microcapsules from mesoporous silica nanoparticles	227:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	0	25	theme	dual	15:18	arg1	gum					55:57	dual network self-healing alginate/guar gum	15:57	dual network self-healing alginate/guar gum	15:57	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	3	26	theme	sodium	626:631	arg1	alginate					633:640	sodium alginate	626:640	sodium alginate	626:640	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	0	27	theme	alginate/guar	41:53	arg1	gum					55:57	dual network self-healing alginate/guar gum	15:57	dual network self-healing alginate/guar gum	15:57	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	5	28	theme	self-healing	917:928	arg1	efficiency					930:939	self-healing efficiency	917:939	self-healing efficiency	917:939	It has also proved that these hydrogels exhibit excellent self-healing performance at ambient temperatures, which self-healing efficiency can reach to 95.5%.
30776441	0	29	dep	hydrogels	59:67	arg1	based					69:73	based	69:73	hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles	59:145	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	4	30	theme	network	715:721	arg1	structure					723:731	The dual network structure	706:731	The dual network structure	706:731	The dual network structure significantly enhances the strength of the hydrogels (up to 7.3 MPa).
30776441	0	31	theme	self-healing	28:39	arg1	gum					55:57	dual network self-healing alginate/guar gum	15:57	dual network self-healing alginate/guar gum	15:57	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	5	32	theme	ambient	889:895	arg1	temperatures					897:908	ambient temperatures	889:908	ambient temperatures	889:908	It has also proved that these hydrogels exhibit excellent self-healing performance at ambient temperatures, which self-healing efficiency can reach to 95.5%.
30776441	1	33	theme	mesoporous	264:273	arg1	nanoparticles					282:294	mesoporous silica nanoparticles	264:294	mesoporous silica nanoparticles	264:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	5	34	theme	excellent	851:859	arg1	performance					874:884	excellent self-healing performance	851:884	excellent self-healing performance	851:884	It has also proved that these hydrogels exhibit excellent self-healing performance at ambient temperatures, which self-healing efficiency can reach to 95.5%.
30776441	1	35	with	hydrogels	212:220	arg1	microcapsules					245:257	polydopamine-type microcapsules	227:257	polydopamine-type microcapsules from mesoporous silica nanoparticles	227:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	3	36	theme	high	551:554	arg1	degree					556:561	a high degree	549:561	a high degree	549:561	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	0	37	theme	gum	55:57	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of dual network self-healing alginate/guar gum	0:57	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	6	38	theme	application	1014:1024	arg1	prospect					1026:1033	a promising application prospect	1002:1033	a promising application prospect for the design and synthesis of functional self-healing materials	1002:1099	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	0	39	from	nanoparticles	133:145	arg1	microcapsules					96:108	polydopamine-type microcapsules	78:108	polydopamine-type microcapsules from mesoporous silica nanoparticles	78:145	Fabrication of dual network self-healing alginate/guar gum hydrogels based on polydopamine-type microcapsules from mesoporous silica nanoparticles.
30776441	3	40	dep	make	512:515	arg1	have					544:547	have	544:547	make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively	512:703	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	3	41	theme	self-healing	566:577	arg1	ability					579:585	self-healing ability	566:585	self-healing ability	566:585	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	6	42	theme	promising	1004:1012	arg1	prospect					1026:1033	a promising application prospect	1002:1033	a promising application prospect for the design and synthesis of functional self-healing materials	1002:1099	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	2	43	theme	crosslinking	377:388	arg1	agent					390:394	a crosslinking agent	375:394	a crosslinking agent	375:394	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	2	43	theme	crosslinking	377:388	arg1	Glutaraldehyde					343:356	Glutaraldehyde	343:356	Glutaraldehyde (GA)	343:361	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	1	44	theme	silica	275:280	arg1	nanoparticles					282:294	mesoporous silica nanoparticles	264:294	mesoporous silica nanoparticles	264:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	1	45	from	nanoparticles	282:294	arg1	microcapsules					245:257	polydopamine-type microcapsules	227:257	polydopamine-type microcapsules from mesoporous silica nanoparticles	227:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
30776441	2	46	used	used	367:370	arg2	GA					359:360	GA	359:360	GA	359:360	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	2	46	used	used	367:370	arg2	Glutaraldehyde					343:356	Glutaraldehyde	343:356	Glutaraldehyde (GA)	343:361	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	2	46	used	used	367:370	arg2	agent					390:394	a crosslinking agent	375:394	a crosslinking agent	375:394	Glutaraldehyde (GA) was used as a crosslinking agent to crosslink guar gum and sodium alginate, respectively.
30776441	3	47	theme	dual	521:524	arg1	hydrogels					534:542	the dual network hydrogels	517:542	the dual network hydrogels	517:542	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	3	48	theme	network	526:532	arg1	hydrogels					534:542	the dual network hydrogels	517:542	the dual network hydrogels	517:542	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	3	49	theme	ability	579:585	arg1	degree					556:561	a high degree	549:561	a high degree	549:561	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	6	50	theme	functional	1067:1076	arg1	materials					1091:1099	functional self-healing materials	1067:1099	functional self-healing materials	1067:1099	The obtained self-healing hydrogels have a promising application prospect for the design and synthesis of functional self-healing materials.
30776441	3	51	theme	microcapsules	677:689	arg1	surface					666:672	the surface	662:672	the surface of microcapsules, respectively	662:703	Metal-ligand interactions as reversible non-covalent bonds make the dual network hydrogels have a high degree of self-healing ability, which FeCl3·6H2O was coordinated with sodium alginate and polydopamine on the surface of microcapsules, respectively.
30776441	1	52	theme	dual	168:171	arg1	hydrogels					212:220	dual network self-healing alginate/guar gum hydrogels	168:220	dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles	168:294	In this manuscript, dual network self-healing alginate/guar gum hydrogels with polydopamine-type microcapsules from mesoporous silica nanoparticles were facilely prepared through one-pot method.
31472224	0	0	theme	simulated	92:100	arg1	digestion					102:110	its simulated digestion	88:110	its simulated digestion in vitro	88:119	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	7	1	theme	digestion	1044:1052	arg1	system					1054:1059	a digestion system	1042:1059	a digestion system of saliva-gastric-small intestinal	1042:1094	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	2	2	theme	%	384:384	arg1	concentrations					364:377	ethanol concentrations	356:377	ethanol concentrations of 20%	356:384	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	0	3	from	characterization	11:26	arg1	margarita					74:82	Fortunella margarita	63:82	Fortunella margarita	63:82	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	0	3	from	characterization	11:26	arg1	digestion					102:110	its simulated digestion	88:110	its simulated digestion in vitro	88:119	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	1	4	from	structure	156:164	arg1	margarita					215:223	the Fortunella margarita	200:223	the Fortunella margarita	200:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	4	5	theme	monosaccharide	682:695	arg1	composition					697:707	a different monosaccharide composition	670:707	a different monosaccharide composition ratio	670:713	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	3	6	with	-α-Galp-	540:547	arg1	point					598:602	a branching point	586:602	a branching point at C6 of β-Manp	586:618	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	5	7	dep	microscope	848:857	arg1	test					859:862	test	859:862	test	859:862	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	8	8	from	structure	1157:1165	arg1	aqueous					1223:1229	aqueous	1223:1229	aqueous	1223:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	7	9	theme	intestinal	1085:1094	arg1	saliva-gastric-small					1064:1083	saliva-gastric-small intestinal	1064:1094	saliva-gastric-small intestinal	1064:1094	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	2	10	theme	polysaccharide	295:308	arg1	fraction					310:317	A novel polysaccharide fraction	287:317	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20)	287:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	6	11	theme	smooth	909:914	arg1	surface					916:922	a smooth surface	907:922	a smooth surface revealed by field emission scanning electron microscopy	907:978	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	8	12	theme	FP20	1118:1121	arg1	digestibility					1101:1113	The digestibility	1097:1113	The digestibility of FP20	1097:1121	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	12	theme	FP20	1118:1121	arg1	related					1127:1133	related	1127:1133	related	1127:1133	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	5	13	theme	atomic	835:840	arg1	microscope					848:857	atomic force microscope	835:857	atomic force microscope test	835:862	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	4	14	theme	plant	765:769	arg1	polysaccharides					771:785	other plant polysaccharides	759:785	other plant polysaccharides	759:785	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	2	15	from	concentrations	364:377	arg1	precipitation					339:351	graded precipitation	332:351	graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20)	332:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	2	16	theme	novel	289:293	arg1	fraction					310:317	A novel polysaccharide fraction	287:317	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20)	287:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	8	17	theme	uniform	1178:1184	arg1	unit					1151:1154	its backbone unit	1138:1154	its backbone unit	1138:1154	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	17	theme	uniform	1178:1184	arg1	conformation					1207:1218	tight, uniform, and spherical chain conformation	1171:1218	tight, uniform, and spherical chain conformation in aqueous	1171:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	3	18	dep	-α-Galp-	540:547	arg1	bone					552:555	1→ bone	549:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	19	theme	β-Manp	613:618	arg1	C6					607:608	C6	607:608	C6 of β-Manp	607:618	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	4	20	theme	other	759:763	arg1	polysaccharides					771:785	other plant polysaccharides	759:785	other plant polysaccharides	759:785	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	4	21	theme	different	672:680	arg1	composition					697:707	a different monosaccharide composition	670:707	a different monosaccharide composition ratio	670:713	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	1	22	theme	polysaccharide	171:184	arg1	fraction					186:193	a polysaccharide fraction	169:193	a polysaccharide fraction from the Fortunella margarita	169:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	1	23	theme	fraction	186:193	arg1	structure					156:164	the structure	152:164	the structure of a polysaccharide fraction from the Fortunella margarita	152:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	1	23	theme	fraction	186:193	arg1	relationship					233:244	the relationship	229:244	the relationship between its digestibility and structure	229:284	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	0	24	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.	0:120	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	8	25	theme	tight	1171:1175	arg1	unit					1151:1154	its backbone unit	1138:1154	its backbone unit	1138:1154	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	25	theme	tight	1171:1175	arg1	conformation					1207:1218	tight, uniform, and spherical chain conformation	1171:1218	tight, uniform, and spherical chain conformation in aqueous	1171:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	2	26	theme	graded	332:337	arg1	precipitation					339:351	graded precipitation	332:351	graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20)	332:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	6	27	contain	had	870:872	arg2	structures					891:900	an island-shaped structures	874:900	an island-shaped structures	874:900	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	6	27	contain	had	870:872	arg1	FP20					865:868	FP20	865:868	FP20	865:868	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	7	28	theme	digestive	1003:1011	arg1	FP20					1019:1022	FP20	1019:1022	FP20	1019:1022	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	7	28	theme	digestive	1003:1011	arg1	test					1013:1016	in vitro digestive test	994:1016	in vitro digestive test	994:1016	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	8	29	from	conformation	1207:1218	arg1	aqueous					1223:1229	aqueous	1223:1229	aqueous	1223:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	3	30	theme	-α-Galp-	525:532	arg1	-α-Galp-					540:547	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	30	theme	-α-Galp-	525:532	arg1	composition					476:486	The unit composition	467:486	The unit composition	467:486	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	31	with	-α-Galp-	568:575	arg1	point					598:602	a branching point	586:602	a branching point at C6 of β-Manp	586:618	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	32	theme	-α-Glcp-	510:517	arg1	-α-Galp-					540:547	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	32	theme	-α-Glcp-	510:517	arg1	composition					476:486	The unit composition	467:486	The unit composition	467:486	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	1	33	theme	Fortunella	204:213	arg1	margarita					215:223	the Fortunella margarita	200:223	the Fortunella margarita	200:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	0	34	theme	mannogalactoglucan	39:56	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.	0:120	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	6	35	theme	island-shaped	877:889	arg1	structures					891:900	an island-shaped structures	874:900	an island-shaped structures	874:900	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	7	36	theme	in	994:995	arg1	FP20					1019:1022	FP20	1019:1022	FP20	1019:1022	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	7	36	theme	in	994:995	arg1	test					1013:1016	in vitro digestive test	994:1016	in vitro digestive test	994:1016	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	3	37	theme	1→	549:550	arg1	bone					552:555	1→ bone	549:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	8	38	theme	chain	1201:1205	arg1	unit					1151:1154	its backbone unit	1138:1154	its backbone unit	1138:1154	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	38	theme	chain	1201:1205	arg1	conformation					1207:1218	tight, uniform, and spherical chain conformation	1171:1218	tight, uniform, and spherical chain conformation in aqueous	1171:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	0	39	theme	novel	33:37	arg1	mannogalactoglucan					39:56	a novel mannogalactoglucan	31:56	a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro	31:119	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	4	40	with	mannogalactoglucan	646:663	arg1	residues					736:743	side-chain sugar residues	719:743	side-chain sugar residues	719:743	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	4	40	with	mannogalactoglucan	646:663	arg1	ratio					709:713	a different monosaccharide composition ratio	670:713	a different monosaccharide composition ratio	670:713	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	8	41	theme	backbone	1142:1149	arg1	unit					1151:1154	its backbone unit	1138:1154	its backbone unit	1138:1154	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	41	theme	backbone	1142:1149	arg1	conformation					1207:1218	tight, uniform, and spherical chain conformation	1171:1218	tight, uniform, and spherical chain conformation in aqueous	1171:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	41	theme	backbone	1142:1149	arg1	structure					1157:1165	structure	1157:1165	structure	1157:1165	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	4	42	theme	sugar	730:734	arg1	residues					736:743	side-chain sugar residues	719:743	side-chain sugar residues	719:743	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	1	43	from	margarita	215:223	arg1	fraction					186:193	a polysaccharide fraction	169:193	a polysaccharide fraction from the Fortunella margarita	169:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	1	43	from	margarita	215:223	arg1	structure					156:164	the structure	152:164	the structure of a polysaccharide fraction from the Fortunella margarita	152:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	1	43	from	margarita	215:223	arg1	relationship					233:244	the relationship	229:244	the relationship between its digestibility and structure	229:284	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	8	44	theme	spherical	1191:1199	arg1	unit					1151:1154	its backbone unit	1138:1154	its backbone unit	1138:1154	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	8	44	theme	spherical	1191:1199	arg1	conformation					1207:1218	tight, uniform, and spherical chain conformation	1171:1218	tight, uniform, and spherical chain conformation in aqueous	1171:1229	The digestibility of FP20 was related to its backbone unit, structure and tight, uniform, and spherical chain conformation in aqueous.
31472224	0	45	theme	Fortunella	63:72	arg1	margarita					74:82	Fortunella margarita	63:82	Fortunella margarita	63:82	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	3	46	theme	-β-Glcp-	495:502	arg1	-α-Galp-					540:547	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	46	theme	-β-Glcp-	495:502	arg1	composition					476:486	The unit composition	467:486	The unit composition	467:486	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	0	47	from	margarita	74:82	arg1	mannogalactoglucan					39:56	a novel mannogalactoglucan	31:56	a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro	31:119	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	0	47	from	margarita	74:82	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.	0:120	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	2	48	from	margarita	394:402	arg1	precipitation					339:351	graded precipitation	332:351	graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20)	332:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	3	49	theme	unit	471:474	arg1	composition					476:486	The unit composition	467:486	The unit composition	467:486	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	3	49	theme	unit	471:474	arg1	-α-Galp-					540:547	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	→4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone	492:555	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	7	50	theme	saliva-gastric-small	1064:1083	arg1	system					1054:1059	a digestion system	1042:1059	a digestion system of saliva-gastric-small intestinal	1042:1094	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	5	51	theme	force	842:846	arg1	microscope					848:857	atomic force microscope	835:857	atomic force microscope test	835:862	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	3	52	theme	branching	588:596	arg1	point					598:602	a branching point	586:602	a branching point at C6 of β-Manp	586:618	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	2	53	theme	ethanol	356:362	arg1	concentrations					364:377	ethanol concentrations	356:377	ethanol concentrations of 20%	356:384	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	6	54	theme	electron	960:967	arg1	microscopy					969:978	field emission scanning electron microscopy	936:978	field emission scanning electron microscopy	936:978	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	1	55	from	relationship	233:244	arg1	margarita					215:223	the Fortunella margarita	200:223	the Fortunella margarita	200:223	This study was to investigate the structure of a polysaccharide fraction from the Fortunella margarita and the relationship between its digestibility and structure.
31472224	4	56	theme	composition	697:707	arg1	ratio					709:713	a different monosaccharide composition ratio	670:713	a different monosaccharide composition ratio	670:713	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	5	57	contain	had	803:805	arg1	FP20					798:801	FP20	798:801	FP20	798:801	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	5	57	contain	had	803:805	arg2	aggregations					819:830	a spherical aggregations	807:830	a spherical aggregations	807:830	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	6	58	theme	scanning	951:958	arg1	microscopy					969:978	field emission scanning electron microscopy	936:978	field emission scanning electron microscopy	936:978	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	7	59	dep	in	994:995	arg1	vitro					997:1001	vitro	997:1001	vitro	997:1001	Furthermore, in vitro digestive test, FP20 was resistance to a digestion system of saliva-gastric-small intestinal.
31472224	0	60	from	digestion	102:110	arg1	mannogalactoglucan					39:56	a novel mannogalactoglucan	31:56	a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro	31:119	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	0	60	from	digestion	102:110	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.	0:120	Structural characterization of a novel mannogalactoglucan from Fortunella margarita and its simulated digestion in vitro.
31472224	4	61	theme	side-chain	719:728	arg1	residues					736:743	side-chain sugar residues	719:743	side-chain sugar residues	719:743	FP20 was identified as a mannogalactoglucan with a different monosaccharide composition ratio and side-chain sugar residues compared with other plant polysaccharides.
31472224	2	62	theme	F.	391:392	arg1	margarita					394:402	F. margarita	391:402	F. margarita (named FP20)	391:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	2	62	theme	F.	391:392	arg1	FP20					411:414	named FP20	405:414	named FP20	405:414	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	2	63	theme	named	405:409	arg1	margarita					394:402	F. margarita	391:402	F. margarita (named FP20)	391:415	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	2	63	theme	named	405:409	arg1	FP20					411:414	named FP20	405:414	named FP20	405:414	A novel polysaccharide fraction extracted by graded precipitation at ethanol concentrations of 20% from F. margarita (named FP20) comprised mainly glucose, galactose, and mannose.
31472224	6	64	theme	emission	942:949	arg1	microscopy					969:978	field emission scanning electron microscopy	936:978	field emission scanning electron microscopy	936:978	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31472224	3	65	from	C6	607:608	arg1	point					598:602	a branching point	586:602	a branching point at C6 of β-Manp	586:618	The unit composition was →4)-β-Glcp-(1 → 2)-α-Glcp-(1 → 2)-α-Galp-(1 → 4)-α-Galp-(1→ bone, and in →2)-α-Galp-(1→) with a branching point at C6 of β-Manp.
31472224	5	66	theme	spherical	809:817	arg1	aggregations					819:830	a spherical aggregations	807:830	a spherical aggregations	807:830	Moreover, FP20 had a spherical aggregations by atomic force microscope test.
31472224	6	67	theme	field	936:940	arg1	microscopy					969:978	field emission scanning electron microscopy	936:978	field emission scanning electron microscopy	936:978	FP20 had an island-shaped structures with a smooth surface revealed by field emission scanning electron microscopy.
31518115	9	0	theme	hydrogels	1548:1556	arg1	stiffness					1528:1536	the reduced stiffness	1516:1536	the reduced stiffness of BC-MLG hydrogels	1516:1556	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	6	1	theme	AX	1059:1060	arg1	XG					1063:1064	XG	1063:1064	XG	1063:1064	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	1	2	theme	unique	266:271	arg1	softness					301:308	softness	301:308	softness	301:308	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	2	theme	unique	266:271	arg1	ability					349:355	water-retention ability	333:355	water-retention ability	333:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	2	theme	unique	266:271	arg1	biocompatibility					311:326	biocompatibility	311:326	biocompatibility	311:326	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	2	theme	unique	266:271	arg1	properties					282:291	unique material properties	266:291	unique material properties such as softness, biocompatibility, and water-retention ability	266:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	10	3	theme	hygiene	1937:1943	arg1	products					1945:1952	personal hygiene products	1928:1952	personal hygiene products	1928:1952	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	6	4	from	effect	1049:1054	arg1	order					1094:1098	the short-range order	1078:1098	the short-range order	1078:1098	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	6	4	from	effect	1049:1054	arg1	mobility					1101:1108	mobility	1101:1108	mobility	1101:1108	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	6	4	from	effect	1049:1054	arg1	hydration					1115:1123	hydration	1115:1123	hydration	1115:1123	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	4	5	theme	significant	810:820	arg1	effect					822:827	a significant effect	808:827	a significant effect	808:827	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	10	6	theme	BC-MLG	1767:1772	arg1	hydrogels					1774:1782	BC-MLG hydrogels	1767:1782	BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1767:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	3	7	theme	BC	553:554	arg1	hydrogels					556:564	chemically pure BC hydrogels	537:564	chemically pure BC hydrogels	537:564	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	5	8	theme	advanced	847:854	arg1	methods					872:878	advanced solid-state NMR methods	847:878	advanced solid-state NMR methods	847:878	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	6	9	theme	short-range	1082:1092	arg1	order					1094:1098	the short-range order	1078:1098	the short-range order	1078:1098	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	1	10	theme	complex	193:199	arg1	organization					219:230	a complex three-dimensional organization	191:230	a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability	191:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	8	11	theme	molecular	1337:1345	arg1	weight					1347:1352	high molecular weight	1332:1352	high molecular weight MLG	1332:1356	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	0	12	theme	NMR	136:138	arg1	Spectroscopy					140:151	Advanced NMR Spectroscopy	127:151	Advanced NMR Spectroscopy	127:151	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	8	13	theme	MLG	1354:1356	arg1	presence					1320:1327	the presence	1316:1327	the presence of high molecular weight MLG	1316:1356	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	6	14	theme	XG	1063:1064	arg1	effect					1049:1054	the effect	1045:1054	the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers	1045:1136	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	7	15	theme	bound	1269:1273	arg1	water					1275:1279	bound water	1269:1279	bound water	1269:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	2	16	theme	gels	514:517	arg1	properties					497:506	the macroscopic properties	481:506	the macroscopic properties of BC gels	481:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	9	17	theme	BC	1612:1613	arg1	fibrils					1615:1621	BC fibrils	1612:1621	BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1612:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	6	18	theme	BC	1128:1129	arg1	fibers					1131:1136	BC fibers	1128:1136	BC fibers	1128:1136	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	7	19	theme	BC-XG	1157:1161	arg1	hydrogels					1163:1171	BC-XG hydrogels	1157:1171	BC-XG hydrogels	1157:1171	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	5	20	theme	NMR	868:870	arg1	methods					872:878	advanced solid-state NMR methods	847:878	advanced solid-state NMR methods	847:878	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	2	21	theme	BC	511:512	arg1	gels					514:517	BC gels	511:517	BC gels	511:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	4	22	theme	force	692:696	arg1	microscopy					698:707	Atomic force microscopy	685:707	Atomic force microscopy	685:707	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	10	23	theme	retention	1903:1911	arg1	capacity					1913:1920	high water retention capacity	1892:1920	high water retention capacity (e.g. personal hygiene products)	1892:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	9	24	dep	adsorption	1576:1585	arg1	i					1569:1569	i	1569:1569	i	1569:1569	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	6	25	theme	fibers	1131:1136	arg1	order					1094:1098	the short-range order	1078:1098	the short-range order	1078:1098	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	6	25	theme	fibers	1131:1136	arg1	mobility					1101:1108	mobility	1101:1108	mobility	1101:1108	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	6	25	theme	fibers	1131:1136	arg1	hydration					1115:1123	hydration	1115:1123	hydration	1115:1123	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	5	26	theme	ribbons	927:933	arg1	structure					911:919	the structure	907:919	the structure of BC ribbons	907:933	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	10	27	theme	high	1892:1895	arg1	capacity					1913:1920	high water retention capacity	1892:1920	high water retention capacity (e.g. personal hygiene products)	1892:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	0	28	theme	Bacterial	86:94	arg1	Cellulose					96:104	Bacterial Cellulose	86:104	Bacterial Cellulose	86:104	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	5	29	theme	ultra-high	938:947	arg1	resolution					949:958	ultra-high resolution	938:958	ultra-high resolution	938:958	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	9	30	from	adsorption	1576:1585	arg1	surface					1601:1607	the surface	1597:1607	the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1597:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	4	31	theme	gels	772:775	arg1	stiffness					756:764	the mechanical stiffness	741:764	the mechanical stiffness of BC gels	741:775	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	1	32	theme	biomedical	380:389	arg1	applications					391:402	biomedical applications	380:402	biomedical applications	380:402	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	7	33	theme	higher	1251:1256	arg1	amounts					1258:1264	higher amounts	1251:1264	higher amounts of bound water	1251:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	7	33	theme	higher	1251:1256	arg1	water					1275:1279	bound water	1269:1279	bound water	1269:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	2	34	theme	features	461:468	arg1	understanding					430:442	a poor understanding	423:442	a poor understanding of the molecular features modulating the macroscopic properties of BC gels	423:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	8	35	theme	water	1431:1435	arg1	interactions					1437:1448	water interactions	1431:1448	water interactions with BC	1431:1456	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	0	36	theme	High	0:3	arg1	Glucan					36:41	High Molecular Weight Mixed-Linkage Glucan	0:41	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.	0:152	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	4	37	theme	mechanical	745:754	arg1	stiffness					756:764	the mechanical stiffness	741:764	the mechanical stiffness of BC gels	741:775	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	8	38	with	interactions	1437:1448	arg1	BC					1455:1456	BC	1455:1456	BC	1455:1456	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	0	39	theme	Weight	15:20	arg1	Glucan					36:41	High Molecular Weight Mixed-Linkage Glucan	0:41	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.	0:152	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	9	40	theme	key	1481:1483	arg1	features					1495:1502	two key molecular features	1477:1502	two key molecular features	1477:1502	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	40	theme	key	1481:1483	arg1	adsorption					1576:1585	the adsorption	1572:1585	(i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1568:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	7	41	theme	BC-XG	1232:1236	arg1	gels					1238:1241	BC-XG gels	1232:1241	BC-XG gels	1232:1241	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	3	42	theme	weight	647:652	arg1	glucan					668:673	high molecular weight mixed-linkage glucan	632:673	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	42	theme	weight	647:652	arg1	BC-MLG					676:681	BC-MLG	676:681	BC-MLG	676:681	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	8	43	theme	first	1300:1304	arg1	time					1306:1309	the first time	1296:1309	the first time	1296:1309	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	5	44	theme	water	994:998	arg1	interactions					1000:1011	local mobility and water interactions	975:1011	interactions	1000:1011	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	10	45	with	hydrogels	1774:1782	arg1	properties					1795:1804	novel properties	1789:1804	novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1789:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	10	45	with	hydrogels	1774:1782	arg1	materials					1855:1863	renewable materials	1845:1863	renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1845:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	3	46	theme	high	632:635	arg1	weight					647:652	high molecular weight	632:652	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	0	47	dep	Glucan	36:41	arg1	Characterization					107:122	Characterization	107:122	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.	0:152	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	7	48	theme	area	1213:1216	arg1	fibrils					1184:1190	BC fibrils	1181:1190	BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water	1181:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	9	49	theme	bound	1699:1703	arg1	water					1705:1709	bound water	1699:1709	bound water	1699:1709	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	7	50	theme	increased	1195:1203	arg1	area					1213:1216	increased surface area	1195:1216	increased surface area	1195:1216	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	8	51	theme	high	1332:1335	arg1	weight					1347:1352	high molecular weight	1332:1352	high molecular weight MLG	1332:1356	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	1	52	theme	fibers	245:250	arg1	organization					219:230	a complex three-dimensional organization	191:230	a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability	191:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	0	53	theme	Mechanical	48:57	arg1	Modulator					73:81	a Mechanical and Hydration Modulator	46:81	a Mechanical and Hydration Modulator of Bacterial Cellulose	46:104	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	9	54	theme	BC-MLG	1541:1546	arg1	hydrogels					1548:1556	BC-MLG hydrogels	1541:1556	BC-MLG hydrogels	1541:1556	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	10	55	dep	products	1945:1952	arg1	e.g.					1923:1926	e.g.	1923:1926	e.g.	1923:1926	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	1	56	theme	material	273:280	arg1	softness					301:308	softness	301:308	softness	301:308	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	56	theme	material	273:280	arg1	ability					349:355	water-retention ability	333:355	water-retention ability	333:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	56	theme	material	273:280	arg1	biocompatibility					311:326	biocompatibility	311:326	biocompatibility	311:326	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	56	theme	material	273:280	arg1	properties					282:291	unique material properties	266:291	unique material properties such as softness, biocompatibility, and water-retention ability	266:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	9	57	dep	preorganization	1680:1694	arg1	ii					1672:1673	ii	1672:1673	ii	1672:1673	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	3	58	theme	pure	548:551	arg1	hydrogels					556:564	chemically pure BC hydrogels	537:564	chemically pure BC hydrogels	537:564	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	0	59	theme	Advanced	127:134	arg1	Spectroscopy					140:151	Advanced NMR Spectroscopy	127:151	Advanced NMR Spectroscopy	127:151	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	8	60	theme	weight	1347:1352	arg1	MLG					1354:1356	high molecular weight MLG	1332:1356	high molecular weight MLG	1332:1356	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	6	61	theme	MLG	1071:1073	arg1	effect					1049:1054	the effect	1045:1054	the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers	1045:1136	This has enabled us to unravel the effect of AX, XG, and MLG on the short-range order, mobility, and hydration of BC fibers.
31518115	1	62	theme	three-dimensional	201:217	arg1	organization					219:230	a complex three-dimensional organization	191:230	a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability	191:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	10	63	theme	personal	1928:1935	arg1	products					1945:1952	personal hygiene products	1928:1952	personal hygiene products	1928:1952	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	9	64	theme	MLG	1590:1592	arg1	adsorption					1576:1585	the adsorption	1572:1585	(i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1568:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	64	theme	MLG	1590:1592	arg1	features					1495:1502	two key molecular features	1477:1502	two key molecular features	1477:1502	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	8	65	theme	fibrils	1396:1402	arg1	clusters					1381:1388	clusters	1381:1388	clusters of BC fibrils	1381:1402	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	8	66	theme	clusters	1381:1388	arg1	density					1370:1376	the density	1366:1376	the density of clusters of BC fibrils	1366:1402	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	7	67	theme	water	1275:1279	arg1	amounts					1258:1264	higher amounts	1251:1264	higher amounts of bound water	1251:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	7	67	theme	water	1275:1279	arg1	water					1275:1279	bound water	1269:1279	bound water	1269:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	0	68	theme	Hydration	63:71	arg1	Modulator					73:81	a Mechanical and Hydration Modulator	46:81	a Mechanical and Hydration Modulator of Bacterial Cellulose	46:104	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	5	69	theme	solid-state	856:866	arg1	methods					872:878	advanced solid-state NMR methods	847:878	advanced solid-state NMR methods	847:878	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	9	70	theme	fibrils	1615:1621	arg1	surface					1601:1607	the surface	1597:1607	the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1597:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	8	71	theme	BC	1393:1394	arg1	fibrils					1396:1402	BC fibrils	1393:1402	BC fibrils	1393:1402	We report for the first time that the presence of high molecular weight MLG reduces the density of clusters of BC fibrils and dramatically increases water interactions with BC.
31518115	10	72	theme	renewable	1845:1853	arg1	materials					1855:1863	renewable materials	1845:1863	renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1845:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	10	72	theme	renewable	1845:1853	arg1	properties					1795:1804	novel properties	1789:1804	novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1789:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	5	73	theme	methods	872:878	arg1	combination					832:842	A combination	830:842	A combination of advanced solid-state NMR methods	830:878	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	9	74	theme	network	1659:1665	arg1	preorganization					1680:1694	the preorganization	1676:1694	(ii) the preorganization of bound water by MLG	1671:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	74	theme	network	1659:1665	arg1	formation					1638:1646	the formation	1634:1646	the formation of a dense network	1634:1665	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	4	75	theme	Atomic	685:690	arg1	microscopy					698:707	Atomic force microscopy	685:707	Atomic force microscopy	685:707	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	10	76	theme	water	1897:1901	arg1	capacity					1913:1920	high water retention capacity	1892:1920	high water retention capacity (e.g. personal hygiene products)	1892:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	0	77	theme	Cellulose	96:104	arg1	Modulator					73:81	a Mechanical and Hydration Modulator	46:81	a Mechanical and Hydration Modulator of Bacterial Cellulose	46:104	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	1	78	theme	key	361:363	arg1	importance					365:374	key importance	361:374	key importance for biomedical applications	361:402	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	9	79	theme	dense	1653:1657	arg1	network					1659:1665	a dense network	1651:1665	a dense network	1651:1665	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	4	80	theme	BC	769:770	arg1	gels					772:775	BC gels	769:775	BC gels	769:775	Atomic force microscopy showed that MLG greatly reduced the mechanical stiffness of BC gels, while XG and AX did not exert a significant effect.
31518115	0	81	theme	Molecular	5:13	arg1	Glucan					36:41	High Molecular Weight Mixed-Linkage Glucan	0:41	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.	0:152	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	10	82	theme	novel	1789:1793	arg1	properties					1795:1804	novel properties	1789:1804	novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1789:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	10	82	theme	novel	1789:1793	arg1	materials					1855:1863	renewable materials	1845:1863	renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products)	1845:1953	Hence, we have produced and fully characterized BC-MLG hydrogels with novel properties which could be potentially employed as renewable materials for applications requiring high water retention capacity (e.g. personal hygiene products).
31518115	2	83	theme	molecular	451:459	arg1	features					461:468	the molecular features	447:468	the molecular features modulating the macroscopic properties of BC gels	447:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	0	84	theme	Mixed-Linkage	22:34	arg1	Glucan					36:41	High Molecular Weight Mixed-Linkage Glucan	0:41	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.	0:152	High Molecular Weight Mixed-Linkage Glucan as a Mechanical and Hydration Modulator of Bacterial Cellulose: Characterization by Advanced NMR Spectroscopy.
31518115	5	85	theme	BC	924:925	arg1	ribbons					927:933	BC ribbons	924:933	BC ribbons	924:933	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	3	86	theme	molecular	637:645	arg1	weight					647:652	high molecular weight	632:652	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	2	87	theme	poor	425:428	arg1	understanding					430:442	a poor understanding	423:442	a poor understanding of the molecular features modulating the macroscopic properties of BC gels	423:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	9	88	theme	molecular	1485:1493	arg1	features					1495:1502	two key molecular features	1477:1502	two key molecular features	1477:1502	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	88	theme	molecular	1485:1493	arg1	adsorption					1576:1585	the adsorption	1572:1585	(i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG	1568:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	2	89	theme	macroscopic	485:495	arg1	properties					497:506	the macroscopic properties	481:506	the macroscopic properties of BC gels	481:517	However, there is a poor understanding of the molecular features modulating the macroscopic properties of BC gels.
31518115	3	90	with	hydrogels	556:564	arg1	xyloglucan					608:617	xyloglucan	608:617	xyloglucan (BC-XG)	608:625	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	90	with	hydrogels	556:564	arg1	arabinoxylan					586:597	arabinoxylan	586:597	arabinoxylan (BC-AX)	586:605	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	90	with	hydrogels	556:564	arg1	BC-XG					620:624	BC-XG	620:624	BC-XG	620:624	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	90	with	hydrogels	556:564	arg1	glucan					668:673	high molecular weight mixed-linkage glucan	632:673	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	90	with	hydrogels	556:564	arg1	BC-AX					600:604	BC-AX	600:604	BC-AX	600:604	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	90	with	hydrogels	556:564	arg1	BC-MLG					676:681	BC-MLG	676:681	BC-MLG	676:681	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	7	91	theme	surface	1205:1211	arg1	area					1213:1216	increased surface area	1195:1216	increased surface area	1195:1216	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	1	92	theme	Bacterial	154:162	arg1	BC					175:176	BC	175:176	BC	175:176	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	92	theme	Bacterial	154:162	arg1	cellulose					164:172	Bacterial cellulose	154:172	Bacterial cellulose (BC)	154:177	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	1	93	theme	water-retention	333:347	arg1	ability					349:355	water-retention ability	333:355	water-retention ability	333:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	9	94	theme	reduced	1520:1526	arg1	stiffness					1528:1536	the reduced stiffness	1516:1536	the reduced stiffness of BC-MLG hydrogels	1516:1556	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	95	theme	water	1705:1709	arg1	preorganization					1680:1694	the preorganization	1676:1694	(ii) the preorganization of bound water by MLG	1671:1716	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	9	95	theme	water	1705:1709	arg1	formation					1638:1646	the formation	1634:1646	the formation of a dense network	1634:1665	Our data supports two key molecular features determining the reduced stiffness of BC-MLG hydrogels, that is, (i) the adsorption of MLG on the surface of BC fibrils precluding the formation of a dense network and (ii) the preorganization of bound water by MLG.
31518115	3	96	theme	mixed-linkage	654:666	arg1	glucan					668:673	high molecular weight mixed-linkage glucan	632:673	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	96	theme	mixed-linkage	654:666	arg1	BC-MLG					676:681	BC-MLG	676:681	BC-MLG	676:681	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	1	97	theme	ultrafine	235:243	arg1	fibers					245:250	ultrafine fibers	235:250	ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability	235:355	Bacterial cellulose (BC) consists of a complex three-dimensional organization of ultrafine fibers which provide unique material properties such as softness, biocompatibility, and water-retention ability, of key importance for biomedical applications.
31518115	5	98	theme	local	975:979	arg1	mobility					981:988	local mobility and water interactions	975:1011	mobility	981:988	A combination of advanced solid-state NMR methods allowed us to characterize the structure of BC ribbons at ultra-high resolution and to monitor local mobility and water interactions.
31518115	7	99	theme	BC	1181:1182	arg1	fibrils					1184:1190	BC fibrils	1181:1190	BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water	1181:1279	Results show that BC-XG hydrogels present BC fibrils of increased surface area, which allows BC-XG gels to hold higher amounts of bound water.
31518115	3	100	with	composites	570:579	arg1	xyloglucan					608:617	xyloglucan	608:617	xyloglucan (BC-XG)	608:625	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	100	with	composites	570:579	arg1	arabinoxylan					586:597	arabinoxylan	586:597	arabinoxylan (BC-AX)	586:605	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	100	with	composites	570:579	arg1	BC-XG					620:624	BC-XG	620:624	BC-XG	620:624	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	100	with	composites	570:579	arg1	glucan					668:673	high molecular weight mixed-linkage glucan	632:673	high molecular weight mixed-linkage glucan (BC-MLG)	632:682	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	100	with	composites	570:579	arg1	BC-AX					600:604	BC-AX	600:604	BC-AX	600:604	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31518115	3	100	with	composites	570:579	arg1	BC-MLG					676:681	BC-MLG	676:681	BC-MLG	676:681	We have examined chemically pure BC hydrogels and composites with arabinoxylan (BC-AX), xyloglucan (BC-XG), and high molecular weight mixed-linkage glucan (BC-MLG).
31499989	3	0	theme	host-guest	709:718	arg1	interaction					720:730	the host-guest interaction	705:730	the host-guest interaction	705:730	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	2	1	theme	double	387:392	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	7	2	theme	excellent	1558:1566	arg1	properties					1584:1593	excellent physicochemical properties	1558:1593	excellent physicochemical properties	1558:1593	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	8	3	theme	biomedical	1816:1825	arg1	applications					1827:1838	biomedical applications	1816:1838	biomedical applications of fluorescent LDHs	1816:1858	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	8	4	theme	materials	1897:1905	arg1	fabrication					1720:1730	fabrication	1720:1730	fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials	1720:1905	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	7	5	theme	great	1607:1611	arg1	potential					1613:1621	great potential	1607:1621	great potential for biomedical applications	1607:1649	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	7	6	theme	fluorescent	1528:1538	arg1	composites					1540:1549	the resultant fluorescent composites	1514:1549	the resultant fluorescent composites	1514:1549	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	3	7	theme	Tb3+-doped	539:548	arg1	LDHs					550:553	Tb3+-doped LDHs	539:553	Tb3+-doped LDHs	539:553	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	3	8	used	utilized	596:603	arg2	LDH-Ad					565:570	LDH-Ad	565:570	LDH-Ad	565:570	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	1	9	theme	interest	256:263	arg1	one					240:242	one	240:242	one	240:242	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	1	9	theme	interest	256:263	arg1	interest					256:263	the most interest research focuses recently	247:289	the most interest research focuses recently	247:289	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	7	10	theme	facile	1480:1485	arg1	interaction					1498:1508	a one-pot facile host-guest interaction	1470:1508	a one-pot facile host-guest interaction	1470:1508	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	5	11	theme	polymer	968:974	arg1	LDHs-β-CD-HPG					988:1000	LDHs-β-CD-HPG	988:1000	LDHs-β-CD-HPG	988:1000	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	5	11	theme	polymer	968:974	arg1	composites					976:985	The obtained Tb3+-doped LDHs based polymer composites	933:985	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG)	933:1001	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	8	12	dep	based	1860:1864	arg1	development					1801:1811	the development	1797:1811	the development of biomedical applications of fluorescent LDHs	1797:1858	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	6	13	theme	laser	1269:1273	arg1	imaging					1275:1281	confocal laser imaging	1260:1281	confocal laser imaging	1260:1281	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	1	14	theme	inorganic-organic	164:180	arg1	composites					190:199	fluorescent inorganic-organic polymer composites	152:199	fluorescent inorganic-organic polymer composites	152:199	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	5	15	theme	obtained	937:944	arg1	LDHs-β-CD-HPG					988:1000	LDHs-β-CD-HPG	988:1000	LDHs-β-CD-HPG	988:1000	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	5	15	theme	obtained	937:944	arg1	composites					976:985	The obtained Tb3+-doped LDHs based polymer composites	933:985	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG)	933:1001	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	7	16	theme	above	1295:1299	arg1	results					1301:1307	the above results	1291:1307	the above results	1291:1307	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	1	17	theme	biomedical	205:214	arg1	applications					216:227	biomedical applications	205:227	biomedical applications	205:227	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	8	18	theme	novel	1657:1661	arg1	method					1684:1689	This novel surface modification method	1652:1689	This novel surface modification method	1652:1689	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	4	19	theme	hyperbranched	797:809	arg1	polyglycerol					811:822	the hyperbranched polyglycerol	793:822	the hyperbranched polyglycerol	793:822	Based on the characterization results, we demonstrated that the hyperbranched polyglycerol could be facilely introduced on these fluorescent Tb3+-doped LDHs through the method described in this work.
31499989	0	20	theme	host-guest	109:118	arg1	interaction					120:130	host-guest interaction	109:130	host-guest interaction	109:130	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	7	21	theme	physicochemical	1568:1582	arg1	properties					1584:1593	excellent physicochemical properties	1558:1593	excellent physicochemical properties	1558:1593	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	8	22	theme	modification	1671:1682	arg1	method					1684:1689	This novel surface modification method	1652:1689	This novel surface modification method	1652:1689	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	6	23	theme	polymer	1154:1160	arg1	composites					1162:1171	LDHs-β-CD-HPG polymer composites	1140:1171	LDHs-β-CD-HPG polymer composites	1140:1171	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	2	24	theme	based	412:416	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	0	25	theme	Surface	0:6	arg1	modification					8:19	Surface modification	0:19	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.	0:131	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	2	26	theme	supramolecular	461:474	arg1	chemistry					476:484	a one-pot supramolecular chemistry	451:484	a one-pot supramolecular chemistry	451:484	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	5	27	theme	improved	1011:1018	arg1	dispersibility					1026:1039	improved water dispersibility	1011:1039	improved water dispersibility	1011:1039	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	4	28	theme	fluorescent	862:872	arg1	LDHs					885:888	these fluorescent Tb3+-doped LDHs	856:888	these fluorescent Tb3+-doped LDHs	856:888	Based on the characterization results, we demonstrated that the hyperbranched polyglycerol could be facilely introduced on these fluorescent Tb3+-doped LDHs through the method described in this work.
31499989	0	29	theme	Tb3+-doped	36:45	arg1	hydroxides					62:71	fluorescent Tb3+-doped layered double hydroxides	24:71	fluorescent Tb3+-doped layered double hydroxides	24:71	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	6	30	theme	various	1101:1107	arg1	assays					1120:1125	various biological assays	1101:1125	various biological assays	1101:1125	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	3	31	theme	hyperbranched	658:670	arg1	polyglycerols					672:684	hyperbranched polyglycerols	658:684	hyperbranched polyglycerols (β-CD-HPG)	658:695	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	3	31	theme	hyperbranched	658:670	arg1	β-CD-HPG					687:694	β-CD-HPG	687:694	β-CD-HPG	687:694	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	2	32	theme	layered	379:385	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	0	33	theme	double	55:60	arg1	hydroxides					62:71	fluorescent Tb3+-doped layered double hydroxides	24:71	fluorescent Tb3+-doped layered double hydroxides	24:71	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	6	34	theme	cell	1207:1210	arg1	behavior					1219:1226	their cell uptake behavior	1201:1226	their cell uptake behavior	1201:1226	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	8	35	theme	multifunctional	1741:1755	arg1	composites					1757:1766	other multifunctional composites	1735:1766	other multifunctional composites	1735:1766	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	2	36	theme	Tb3+-doped	356:365	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	8	37	theme	applications	1827:1838	arg1	development					1801:1811	the development	1797:1811	the development of biomedical applications of fluorescent LDHs	1797:1858	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	1	38	theme	composites	190:199	arg1	preparation					137:147	The preparation	133:147	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications	133:227	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	3	39	theme	LDHs	550:553	arg1	surface					528:534	the surface	524:534	the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction	524:730	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	8	40	theme	fluorescent	1843:1853	arg1	LDHs					1855:1858	fluorescent LDHs	1843:1858	fluorescent LDHs	1843:1858	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	7	41	theme	biomedical	1627:1636	arg1	applications					1638:1649	biomedical applications	1627:1649	biomedical applications	1627:1649	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	6	42	theme	low	1180:1182	arg1	cytotoxicity					1184:1195	low cytotoxicity	1180:1195	low cytotoxicity	1180:1195	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	8	43	theme	great	1782:1786	arg1	composites					1874:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	2	44	theme	hydroxides	394:403	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	8	45	theme	based	1860:1864	arg1	composites					1874:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	2	46	theme	novel	320:324	arg1	method					326:331	a novel method	318:331	a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	318:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	8	47	theme	composites	1874:1883	arg1	fabrication					1720:1730	fabrication	1720:1730	fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials	1720:1905	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	6	48	theme	confocal	1260:1267	arg1	imaging					1275:1281	confocal laser imaging	1260:1281	confocal laser imaging	1260:1281	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	6	49	theme	uptake	1212:1217	arg1	behavior					1219:1226	their cell uptake behavior	1201:1226	their cell uptake behavior	1201:1226	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	8	50	theme	related	1889:1895	arg1	materials					1897:1905	related materials	1889:1905	related materials	1889:1905	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	5	51	theme	LDHs	957:960	arg1	LDHs-β-CD-HPG					988:1000	LDHs-β-CD-HPG	988:1000	LDHs-β-CD-HPG	988:1000	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	5	51	theme	LDHs	957:960	arg1	composites					976:985	The obtained Tb3+-doped LDHs based polymer composites	933:985	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG)	933:1001	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	3	52	contain	containing	647:656	arg1	β-CD					641:644	β-CD	641:644	β-CD	641:644	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	3	52	contain	containing	647:656	arg2	β-CD-HPG					687:694	β-CD-HPG	687:694	β-CD-HPG	687:694	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	3	52	contain	containing	647:656	arg1	β-cyclodextrin					625:638	the β-cyclodextrin	621:638	the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction	621:730	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	3	52	contain	containing	647:656	arg2	polyglycerols					672:684	hyperbranched polyglycerols	658:684	hyperbranched polyglycerols (β-CD-HPG)	658:695	The adamantane can be immobilized on the surface of Tb3+-doped LDHs to obtain LDH-Ad, which could be further utilized for modified by the β-cyclodextrin (β-CD) containing hyperbranched polyglycerols (β-CD-HPG) through the host-guest interaction.
31499989	1	53	theme	most	251:254	arg1	interest					256:263	the most interest research focuses recently	247:289	the most interest research focuses recently	247:289	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	0	54	mod	modification	8:19	arg3	Surface					0:6	Surface modification	0:19	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.	0:131	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	0	54	mod	modification	8:19	arg1	hydroxides					62:71	fluorescent Tb3+-doped layered double hydroxides	24:71	fluorescent Tb3+-doped layered double hydroxides	24:71	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	5	55	theme	based	962:966	arg1	LDHs-β-CD-HPG					988:1000	LDHs-β-CD-HPG	988:1000	LDHs-β-CD-HPG	988:1000	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	5	55	theme	based	962:966	arg1	composites					976:985	The obtained Tb3+-doped LDHs based polymer composites	933:985	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG)	933:1001	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	7	56	theme	host-guest	1487:1496	arg1	interaction					1498:1508	a one-pot facile host-guest interaction	1470:1508	a one-pot facile host-guest interaction	1470:1508	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	1	57	theme	fluorescent	152:162	arg1	composites					190:199	fluorescent inorganic-organic polymer composites	152:199	fluorescent inorganic-organic polymer composites	152:199	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	7	58	theme	one-pot	1472:1478	arg1	interaction					1498:1508	a one-pot facile host-guest interaction	1470:1508	a one-pot facile host-guest interaction	1470:1508	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	4	59	theme	characterization	746:761	arg1	results					763:769	the characterization results	742:769	the characterization results	742:769	Based on the characterization results, we demonstrated that the hyperbranched polyglycerol could be facilely introduced on these fluorescent Tb3+-doped LDHs through the method described in this work.
31499989	0	60	theme	hyperbranched	78:90	arg1	polymers					92:99	hyperbranched polymers	78:99	hyperbranched polymers through host-guest interaction	78:130	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	1	61	theme	polymer	182:188	arg1	composites					190:199	fluorescent inorganic-organic polymer composites	152:199	fluorescent inorganic-organic polymer composites	152:199	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	7	62	mod	modified	1413:1420	arg3	polymers					1453:1460	hydrophilic hyperbranched polymers	1427:1460	hydrophilic hyperbranched polymers	1427:1460	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	7	62	mod	modified	1413:1420	arg1	composites					1373:1382	polymer composites	1365:1382	polymer composites	1365:1382	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	5	63	theme	Tb3+-doped	946:955	arg1	LDHs-β-CD-HPG					988:1000	LDHs-β-CD-HPG	988:1000	LDHs-β-CD-HPG	988:1000	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	5	63	theme	Tb3+-doped	946:955	arg1	composites					976:985	The obtained Tb3+-doped LDHs based polymer composites	933:985	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG)	933:1001	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	8	64	theme	surface	1663:1669	arg1	method					1684:1689	This novel surface modification method	1652:1689	This novel surface modification method	1652:1689	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	5	65	theme	water	1020:1024	arg1	dispersibility					1026:1039	improved water dispersibility	1011:1039	improved water dispersibility	1011:1039	The obtained Tb3+-doped LDHs based polymer composites (LDHs-β-CD-HPG) display improved water dispersibility and still maintain their fluorescence.
31499989	7	66	theme	resultant	1518:1526	arg1	composites					1540:1549	the resultant fluorescent composites	1514:1549	the resultant fluorescent composites	1514:1549	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	7	67	theme	hydrophilic	1427:1437	arg1	polymers					1453:1460	hydrophilic hyperbranched polymers	1427:1460	hydrophilic hyperbranched polymers	1427:1460	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	2	68	theme	composites	418:427	arg1	preparation					341:351	the preparation	337:351	the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	337:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	6	69	theme	LDHs-β-CD-HPG	1140:1152	arg1	composites					1162:1171	LDHs-β-CD-HPG polymer composites	1140:1171	LDHs-β-CD-HPG polymer composites	1140:1171	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	2	70	theme	chemistry	476:484	arg1	advantage					438:446	advantage	438:446	advantage of a one-pot supramolecular chemistry	438:484	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	0	71	theme	fluorescent	24:34	arg1	hydroxides					62:71	fluorescent Tb3+-doped layered double hydroxides	24:71	fluorescent Tb3+-doped layered double hydroxides	24:71	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	2	72	theme	one-pot	453:459	arg1	chemistry					476:484	a one-pot supramolecular chemistry	451:484	a one-pot supramolecular chemistry	451:484	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	2	73	theme	LDHs	406:409	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	0	74	theme	layered	47:53	arg1	hydroxides					62:71	fluorescent Tb3+-doped layered double hydroxides	24:71	fluorescent Tb3+-doped layered double hydroxides	24:71	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	7	75	dep	modified	1413:1420	arg1	surface					1405:1411	surface	1405:1411	surface	1405:1411	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	6	76	theme	biological	1109:1118	arg1	assays					1120:1125	various biological assays	1101:1125	various biological assays	1101:1125	The results based on various biological assays suggest that LDHs-β-CD-HPG polymer composites are of low cytotoxicity and their cell uptake behavior can be effectively traced using confocal laser imaging.
31499989	8	77	theme	other	1735:1739	arg1	composites					1757:1766	other multifunctional composites	1735:1766	other multifunctional composites	1735:1766	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	0	78	theme	hydroxides	62:71	arg1	modification					8:19	Surface modification	0:19	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.	0:131	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	7	79	theme	polymer	1365:1371	arg1	composites					1373:1382	polymer composites	1365:1382	polymer composites	1365:1382	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	8	80	theme	composites	1757:1766	arg1	fabrication					1720:1730	fabrication	1720:1730	fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials	1720:1905	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	4	81	theme	Tb3+-doped	874:883	arg1	LDHs					885:888	these fluorescent Tb3+-doped LDHs	856:888	these fluorescent Tb3+-doped LDHs	856:888	Based on the characterization results, we demonstrated that the hyperbranched polyglycerol could be facilely introduced on these fluorescent Tb3+-doped LDHs through the method described in this work.
31499989	0	82	with	modification	8:19	arg1	polymers					92:99	hyperbranched polymers	78:99	hyperbranched polymers through host-guest interaction	78:130	Surface modification of fluorescent Tb3+-doped layered double hydroxides with hyperbranched polymers through host-guest interaction.
31499989	2	83	theme	luminescent	367:377	arg1	composites					418:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	Tb3+-doped luminescent layered double hydroxides (LDHs) based composites	356:427	In this work, we reported a novel method for the preparation of Tb3+-doped luminescent layered double hydroxides (LDHs) based composites by taken advantage of a one-pot supramolecular chemistry.
31499989	1	84	dep	interest	256:263	arg1	focuses					274:280	focuses	274:280	focuses recently	274:289	The preparation of fluorescent inorganic-organic polymer composites for biomedical applications has become one of the most interest research focuses recently.
31499989	7	85	theme	Tb3+-doped	1343:1352	arg1	LDHs					1354:1357	the fluorescent Tb3+-doped LDHs	1327:1357	the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites	1327:1549	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	8	86	theme	LDHs	1855:1858	arg1	applications					1827:1838	biomedical applications	1816:1838	biomedical applications of fluorescent LDHs	1816:1858	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	7	87	theme	fluorescent	1331:1341	arg1	LDHs					1354:1357	the fluorescent Tb3+-doped LDHs	1327:1357	the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites	1327:1549	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	7	88	theme	hyperbranched	1439:1451	arg1	polymers					1453:1460	hydrophilic hyperbranched polymers	1427:1460	hydrophilic hyperbranched polymers	1427:1460	All of the above results demonstrated that the fluorescent Tb3+-doped LDHs based polymer composites could be effectively surface modified with hydrophilic hyperbranched polymers through a one-pot facile host-guest interaction and the resultant fluorescent composites are of excellent physicochemical properties and display great potential for biomedical applications.
31499989	8	89	theme	advanced	1788:1795	arg1	composites					1874:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
31499989	8	90	theme	polymer	1866:1872	arg1	composites					1874:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	great advanced the development of biomedical applications of fluorescent LDHs based polymer composites	1782:1883	This novel surface modification method should also be important for fabrication of other multifunctional composites and therefore great advanced the development of biomedical applications of fluorescent LDHs based polymer composites and related materials.
30679893	8	0	theme	many	1761:1764	arg1	issues					1776:1781	many remaining issues	1761:1781	many remaining issues	1761:1781	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	5	1	theme	developmental	1237:1249	arg1	malignancy					1258:1267	malignancy	1258:1267	malignancy	1258:1267	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	1	theme	developmental	1237:1249	arg1	stage					1251:1255	developmental stage	1237:1255	developmental stage	1237:1255	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	4	2	dep	genes	943:947	arg1	sulfotransferases					1007:1023	sulfotransferases	1007:1023	sulfotransferases	1007:1023	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	2	dep	genes	943:947	arg1	glycosyltransferases					985:1004	glycosyltransferases	985:1004	glycosyltransferases	985:1004	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	2	dep	genes	943:947	arg1	transporters					1043:1054	nucleotide sugar transporters	1026:1054	nucleotide sugar transporters	1026:1054	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	7	3	dep	"	1646:1646	arg1	i.e.					1628:1631	i.e.	1628:1631	i.e.	1628:1631	As a result, most of nonglycoscientists tend to hesitate glycomics, i.e., "glycophobia".
30679893	4	4	dep	>200	893:896	arg1	e.g.					887:890	e.g.	887:890	e.g.	887:890	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	5	5	theme	good	1288:1291	arg1	marker					1293:1298	a good marker	1286:1298	a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9)	1286:1389	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	5	theme	good	1288:1291	arg1	glycans					1271:1277	glycans	1271:1277	glycans	1271:1277	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	2	6	theme	glycans	219:225	arg1	known[1					251:257	known[1	251:257	known[1	251:257	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	2	6	theme	glycans	219:225	arg1	origin					209:214	The origin	205:214	The origin of glycans or carbohydrates	205:242	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	3	7	theme	major	781:785	arg1	glycosylation					764:776	glycosylation	764:776	glycosylation	764:776	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	7	theme	major	781:785	arg1	event					787:791	a major event	779:791	a major event of posttranslational modifications	779:826	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	1	8	theme	glycans	196:202	arg1	layer					187:191	a dense layer	179:191	a dense layer of glycans	179:202	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	7	9	theme	glycophobia	1635:1645	arg1	"					1646:1646	"glycophobia"	1634:1646	"glycophobia"	1634:1646	As a result, most of nonglycoscientists tend to hesitate glycomics, i.e., "glycophobia".
30679893	1	10	dep	organisms	110:118	arg1	bacteria					154:161	bacteria	154:161	bacteria	154:161	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	1	10	dep	organisms	110:118	arg1	fungi					144:148	fungi	144:148	fungi	144:148	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	1	10	dep	organisms	110:118	arg1	plants					136:141	plants	136:141	plants	136:141	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	1	10	dep	organisms	110:118	arg1	animals					127:133	animals	127:133	animals	127:133	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	3	11	dep	glycoconjugates	583:597	arg1	i.e.					577:580	i.e.	577:580	i.e.	577:580	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	5	12	theme	cell	1304:1307	arg1	SSEA-1					1323:1328	SSEA-1	1323:1328	SSEA-1	1323:1328	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	12	theme	cell	1304:1307	arg1	typing					1309:1314	cell typing	1304:1314	cell typing (e.g., SSEA-1)	1304:1329	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	8	13	theme	remaining	1766:1774	arg1	issues					1776:1781	many remaining issues	1761:1781	many remaining issues	1761:1781	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	5	14	dep	diagnosis	1341:1349	arg1	biomarker					1365:1373	cancer biomarker	1358:1373	cancer biomarker such as CA19-9	1358:1388	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	9	15	theme	regenerative	1920:1931	arg1	medicine[3					1933:1942	regenerative medicine[3	1920:1942	regenerative medicine[3	1920:1942	In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.
30679893	4	16	theme	different	1092:1100	arg1	conditions					1102:1111	different conditions	1092:1111	different conditions	1092:1111	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	3	17	theme	posttranslational	796:812	arg1	modifications					814:826	posttranslational modifications	796:826	posttranslational modifications	796:826	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	4	18	from	>200	893:896	arg1	human					901:905	human	901:905	human	901:905	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	19	theme	glycol-genes	908:919	arg1	series					876:881	a series	874:881	a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions	874:1111	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	3	20	theme	lumen	629:633	arg1	apparatus					690:698	the following Golgi apparatus	670:698	the following Golgi apparatus	670:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	20	theme	lumen	629:633	arg1	sites					635:639	lumen sites	629:639	lumen sites of endoplasmic reticulum and the following Golgi apparatus	629:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	20	theme	lumen	629:633	arg1	reticulum					656:664	endoplasmic reticulum	644:664	endoplasmic reticulum	644:664	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	9	21	theme	plenary	1811:1817	arg1	lecture					1819:1825	this plenary lecture	1806:1825	this plenary lecture	1806:1825	In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.
30679893	3	22	theme	modifications	814:826	arg1	glycosylation					764:776	glycosylation	764:776	glycosylation	764:776	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	22	theme	modifications	814:826	arg1	event					787:791	a major event	779:791	a major event of posttranslational modifications	779:826	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	2	23	theme	biological	350:359	arg1	phenomena					361:369	various biological phenomena	342:369	various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection	342:501	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	8	24	theme	important	1686:1694	arg1	glycoscience					1663:1674	glycoscience	1663:1674	glycoscience	1663:1674	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	8	24	theme	important	1686:1694	arg1	field					1696:1700	a very important field	1679:1700	a very important field	1679:1700	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	1	25	dep	animals	127:133	arg1	i.e.					121:124	i.e.	121:124	i.e.	121:124	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	3	26	gly	glycoproteins	528:540	arg1	one					543:545	one	543:545	one	543:545	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	26	gly	glycoproteins	528:540	arg1	forms					559:563	existing forms	550:563	existing forms of glycans (i.e., glycoconjugates)	550:598	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	26	gly	glycoproteins	528:540	arg1	glycoproteins					528:540	glycoproteins	528:540	glycoproteins	528:540	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	2	27	theme	various	342:348	arg1	phenomena					361:369	various biological phenomena	342:369	various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection	342:501	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	9	28	theme	novel	1830:1834	arg1	approach					1836:1843	a novel approach	1828:1843	a novel approach to glycan profiling[2	1828:1865	In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.
30679893	1	29	from	kinds	101:105	arg1	cells					86:90	cells	86:90	cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria)	86:162	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	1	29	from	kinds	101:105	arg1	kinds					77:81	All kinds	73:81	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria)	73:162	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	2	30	theme	carbohydrates	230:242	arg1	known[1					251:257	known[1	251:257	known[1	251:257	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	2	30	theme	carbohydrates	230:242	arg1	origin					209:214	The origin	205:214	The origin of glycans or carbohydrates	205:242	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	5	31	theme	biological	1192:1201	arg1	origin					1203:1208	biological origin	1192:1208	biological origin	1192:1208	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	32	dep	SSEA-1	1323:1328	arg1	e.g.					1317:1320	e.g.	1317:1320	e.g.	1317:1320	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	3	33	theme	endoplasmic	644:654	arg1	reticulum					656:664	endoplasmic reticulum	644:664	endoplasmic reticulum	644:664	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	5	34	theme	cancer	1358:1363	arg1	biomarker					1365:1373	cancer biomarker	1358:1373	cancer biomarker such as CA19-9	1358:1388	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	6	35	theme	major	1512:1516	arg1	disciplines					1518:1528	other major disciplines	1506:1528	other major disciplines like genomics and proteomics	1506:1557	However, glycan preparation as well as its analysis and total understanding are much more difficult compared with other major disciplines like genomics and proteomics.
30679893	0	36	theme	cellular	33:40	arg1	aging					42:46	cellular aging	33:46	cellular aging	33:46	An insight into pluripotency and cellular aging through glycan analysis.
30679893	3	37	theme	reticulum	656:664	arg1	apparatus					690:698	the following Golgi apparatus	670:698	the following Golgi apparatus	670:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	37	theme	reticulum	656:664	arg1	sites					635:639	lumen sites	629:639	lumen sites of endoplasmic reticulum and the following Golgi apparatus	629:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	37	theme	reticulum	656:664	arg1	reticulum					656:664	endoplasmic reticulum	644:664	endoplasmic reticulum	644:664	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	38	theme	glycans	568:574	arg1	forms					559:563	existing forms	550:563	existing forms of glycans (i.e., glycoconjugates)	550:598	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	2	39	theme	cellular	380:387	arg1	communications					389:402	cellular communications	380:402	cellular communications	380:402	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	3	40	theme	apparatus	690:698	arg1	apparatus					690:698	the following Golgi apparatus	670:698	the following Golgi apparatus	670:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	40	theme	apparatus	690:698	arg1	sites					635:639	lumen sites	629:639	lumen sites of endoplasmic reticulum and the following Golgi apparatus	629:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	40	theme	apparatus	690:698	arg1	reticulum					656:664	endoplasmic reticulum	644:664	endoplasmic reticulum	644:664	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	4	41	theme	glycan	838:843	arg1	structures					845:854	glycan structures	838:854	glycan structures	838:854	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	3	42	theme	existing	550:557	arg1	forms					559:563	existing forms	550:563	existing forms of glycans (i.e., glycoconjugates)	550:598	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	4	43	dep	glycosyltransferases	985:1004	arg1	e.g.					979:982	e.g.	979:982	e.g.	979:982	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	8	44	from	field	1696:1700	arg1	future					1739:1744	future	1739:1744	future	1739:1744	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	5	45	theme	serum	1335:1339	arg1	diagnosis					1341:1349	serum diagnosis	1335:1349	serum diagnosis (e.g., cancer biomarker such as CA19-9)	1335:1389	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	3	46	theme	forms	559:563	arg1	one					543:545	one	543:545	one	543:545	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	46	theme	forms	559:563	arg1	forms					559:563	existing forms	550:563	existing forms of glycans (i.e., glycoconjugates)	550:598	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	46	theme	forms	559:563	arg1	glycoproteins					528:540	glycoproteins	528:540	glycoproteins	528:540	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	6	47	theme	glycan	1401:1406	arg1	preparation					1408:1418	glycan preparation	1401:1418	glycan preparation as well as its analysis and total understanding	1401:1466	However, glycan preparation as well as its analysis and total understanding are much more difficult compared with other major disciplines like genomics and proteomics.
30679893	8	48	theme	science	1710:1716	arg1	glycoscience					1663:1674	glycoscience	1663:1674	glycoscience	1663:1674	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	8	48	theme	science	1710:1716	arg1	field					1696:1700	a very important field	1679:1700	a very important field	1679:1700	Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved.
30679893	3	49	theme	following	674:682	arg1	apparatus					690:698	the following Golgi apparatus	670:698	the following Golgi apparatus	670:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	9	50	theme	glycan	1848:1853	arg1	profiling[2					1855:1865	glycan profiling[2	1848:1865	glycan profiling[2	1848:1865	In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.
30679893	5	51	theme	cell	1174:1177	arg1	types					1179:1183	different cell types	1164:1183	different cell types (e.g., biological origin, tissue)	1164:1217	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	51	theme	cell	1174:1177	arg1	tissue					1211:1216	tissue	1211:1216	tissue	1211:1216	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	3	52	theme	Golgi	684:688	arg1	apparatus					690:698	the following Golgi apparatus	670:698	the following Golgi apparatus	670:698	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	9	53	theme	biomarker	1892:1900	arg1	investigation					1902:1914	biomarker investigation	1892:1914	biomarker investigation	1892:1914	In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.
30679893	6	54	theme	other	1506:1510	arg1	disciplines					1518:1528	other major disciplines	1506:1528	other major disciplines like genomics and proteomics	1506:1557	However, glycan preparation as well as its analysis and total understanding are much more difficult compared with other major disciplines like genomics and proteomics.
30679893	6	55	theme	total	1448:1452	arg1	understanding					1454:1466	total understanding	1448:1466	total understanding	1448:1466	However, glycan preparation as well as its analysis and total understanding are much more difficult compared with other major disciplines like genomics and proteomics.
30679893	1	56	theme	cells	86:90	arg1	kinds					77:81	All kinds	73:81	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria)	73:162	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	3	57	theme	cytoplasmic	715:725	arg1	proteins					727:734	cytoplasmic proteins	715:734	cytoplasmic proteins	715:734	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	3	58	from	proteins	727:734	arg1	distinct					701:708	distinct	701:708	distinct	701:708	It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications.
30679893	5	59	dep	origin	1203:1208	arg1	e.g.					1186:1189	e.g.	1186:1189	e.g.	1186:1189	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	1	60	theme	organisms	110:118	arg1	kinds					101:105	all kinds	97:105	all kinds of organisms (i.e., animals, plants, fungi and bacteria)	97:162	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	1	61	from	kinds	77:81	arg1	kinds					101:105	all kinds	97:105	all kinds of organisms (i.e., animals, plants, fungi and bacteria)	97:162	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30679893	4	62	theme	nucleotide	1026:1035	arg1	glycosyltransferases					985:1004	glycosyltransferases	985:1004	glycosyltransferases	985:1004	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	62	theme	nucleotide	1026:1035	arg1	transporters					1043:1054	nucleotide sugar transporters	1026:1054	nucleotide sugar transporters	1026:1054	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	5	63	dep	biomarker	1365:1373	arg1	e.g.					1352:1355	e.g.	1352:1355	e.g.	1352:1355	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	64	theme	different	1164:1172	arg1	types					1179:1183	different cell types	1164:1183	different cell types (e.g., biological origin, tissue)	1164:1217	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	5	64	theme	different	1164:1172	arg1	tissue					1211:1216	tissue	1211:1216	tissue	1211:1216	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	4	65	theme	sugar	1037:1041	arg1	glycosyltransferases					985:1004	glycosyltransferases	985:1004	glycosyltransferases	985:1004	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	65	theme	sugar	1037:1041	arg1	transporters					1043:1054	nucleotide sugar transporters	1026:1054	nucleotide sugar transporters	1026:1054	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	4	66	theme	glycan	961:966	arg1	synthesis					968:976	glycan synthesis	961:976	glycan synthesis	961:976	In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions.
30679893	0	67	theme	glycan	56:61	arg1	analysis					63:70	glycan analysis	56:70	glycan analysis	56:70	An insight into pluripotency and cellular aging through glycan analysis.
30679893	5	68	dep	stage	1251:1255	arg1	e.g.					1231:1234	e.g.	1231:1234	e.g.	1231:1234	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	2	69	theme	above	274:278	arg1	fact					280:283	the above fact	270:283	the above fact	270:283	The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection.
30679893	5	70	theme	glycol-gene	1141:1151	arg1	expression					1122:1131	expression	1122:1131	expression of each glycol-gene	1122:1151	Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9).
30679893	1	71	theme	dense	181:185	arg1	layer					187:191	a dense layer	179:191	a dense layer of glycans	179:202	All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans.
30582698	3	0	theme	C-type	320:325	arg1	lectin					374:379	the macrophage galactose-type lectin	344:379	the macrophage galactose-type lectin (MGL)	344:385	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	0	theme	C-type	320:325	arg1	lectins					327:333	Other C-type lectins	314:333	Other C-type lectins	314:333	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	1	1	theme	human	144:148	arg1	processes					181:189	many human physiological and pathological processes	139:189	many human physiological and pathological processes	139:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	5	2	theme	understanding	722:734	arg1	lack					714:717	a lack	712:717	a lack of understanding of the actual glycoproteins that bind MGL	712:776	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	0	3	from	Analysis	15:22	arg1	Cells					73:77	Acute T-Cell Leukemia Cells	51:77	Acute T-Cell Leukemia Cells	51:77	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	4	4	located	found	615:619	arg1	cells					634:638	malignant cells	624:638	malignant cells	624:638	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	4	4	located	found	615:619	arg2	antigen					588:594	the Tn antigen	581:594	the Tn antigen	581:594	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	8	5	theme	these	1134:1138	arg1	several					1123:1129	several	1123:1129	several	1123:1129	Importantly, for several of these, O-glycosylation has hitherto not been described.
30582698	1	6	theme	physiological	150:162	arg1	processes					181:189	many human physiological and pathological processes	139:189	many human physiological and pathological processes	139:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	7	7	from	proteins	1003:1010	arg1	cells					1035:1039	these cells	1029:1039	these cells	1029:1039	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	3	8	theme	macrophage	348:357	arg1	MGL					382:384	MGL	382:384	MGL	382:384	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	8	theme	macrophage	348:357	arg1	lectin					374:379	the macrophage galactose-type lectin	344:379	the macrophage galactose-type lectin (MGL)	344:385	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	9	9	theme	cancer	1337:1342	arg1	responses					1351:1359	cancer immune responses	1337:1359	cancer immune responses	1337:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	3	10	theme	galactose-type	359:372	arg1	MGL					382:384	MGL	382:384	MGL	382:384	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	10	theme	galactose-type	359:372	arg1	lectin					374:379	the macrophage galactose-type lectin	344:379	the macrophage galactose-type lectin (MGL)	344:385	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	9	11	theme	new	1219:1221	arg1	insight					1223:1229	new insight	1219:1229	new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses	1219:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	7	12	theme	known	961:965	arg1	ligands					971:977	known MGL ligands	961:977	known MGL ligands	961:977	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	6	13	theme	MGL	890:892	arg1	ligands					894:900	MGL ligands	890:900	MGL ligands on the human Jurkat leukemia cell line	890:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	2	14	theme	immune	278:283	arg1	responses					285:293	innate immune responses	271:293	innate immune responses against pathogens	271:311	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	6	15	theme	cell	931:934	arg1	line					936:939	the human Jurkat leukemia cell line	905:939	the human Jurkat leukemia cell line	905:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	6	16	theme	proteins	854:861	arg1	identification					824:837	the identification	820:837	the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line	820:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	2	17	theme	innate	271:276	arg1	responses					285:293	innate immune responses	271:293	innate immune responses against pathogens	271:311	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	3	18	theme	immunosuppressive	414:430	arg1	responses					432:440	immunosuppressive responses	414:440	immunosuppressive responses	414:440	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	5	19	theme	glycoproteins	750:762	arg1	understanding					722:734	understanding	722:734	understanding of the actual glycoproteins that bind MGL	722:776	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	6	20	theme	MGL-binding	842:852	arg1	proteins					854:861	MGL-binding proteins	842:861	MGL-binding proteins	842:861	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	1	21	theme	C-type	80:85	arg1	proteins					118:125	proteins	118:125	proteins involved in many human physiological and pathological processes	118:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	21	theme	C-type	80:85	arg1	group					109:113	a diverse group	99:113	a diverse group of proteins involved in many human physiological and pathological processes	99:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	21	theme	C-type	80:85	arg1	lectins					87:93	C-type lectins	80:93	C-type lectins	80:93	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	22	theme	pathological	168:179	arg1	processes					181:189	many human physiological and pathological processes	139:189	many human physiological and pathological processes	139:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	7	23	theme	Tn	983:984	arg1	proteins					1003:1010	Tn antigen-carrying proteins CD43 and CD45	983:1024	Tn antigen-carrying proteins CD43 and CD45	983:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	23	theme	Tn	983:984	arg1	CD45					1021:1024	CD45	1021:1024	CD45	1021:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	23	theme	Tn	983:984	arg1	CD43					1012:1015	CD43	1012:1015	CD43	1012:1015	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	0	24	theme	Glycoproteomic	0:13	arg1	Analysis					15:22	Glycoproteomic Analysis	0:22	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.	0:78	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	6	25	theme	leukemia	922:929	arg1	line					936:939	the human Jurkat leukemia cell line	905:939	the human Jurkat leukemia cell line	905:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	9	26	theme	ligands	1282:1288	arg1	structure					1259:1267	structure	1259:1267	structure	1259:1267	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	9	26	theme	ligands	1282:1288	arg1	identification					1240:1253	identification	1240:1253	identification	1240:1253	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	0	27	theme	MGL-Binding	27:37	arg1	Proteins					39:46	MGL-Binding Proteins	27:46	MGL-Binding Proteins	27:46	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	6	28	theme	Jurkat	915:920	arg1	line					936:939	the human Jurkat leukemia cell line	905:939	the human Jurkat leukemia cell line	905:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	5	29	theme	actual	743:748	arg1	glycoproteins					750:762	the actual glycoproteins	739:762	the actual glycoproteins that bind MGL	739:776	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	7	30	theme	ligands	1089:1095	arg1	ligands					1089:1095	novel cell-surface ligands	1070:1095	novel cell-surface ligands for MGL	1070:1103	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	30	theme	ligands	1089:1095	arg1	set					1063:1065	a set	1061:1065	a set of novel cell-surface ligands for MGL	1061:1103	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	6	31	theme	human	909:913	arg1	line					936:939	the human Jurkat leukemia cell line	905:939	the human Jurkat leukemia cell line	905:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	5	32	theme	MGL	680:682	arg1	specificity					665:675	this glycan specificity	653:675	this glycan specificity of MGL	653:682	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	1	33	theme	diverse	101:107	arg1	proteins					118:125	proteins	118:125	proteins involved in many human physiological and pathological processes	118:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	33	theme	diverse	101:107	arg1	group					109:113	a diverse group	99:113	a diverse group of proteins involved in many human physiological and pathological processes	99:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	33	theme	diverse	101:107	arg1	lectins					87:93	C-type lectins	80:93	C-type lectins	80:93	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	9	34	theme	immune	1344:1349	arg1	responses					1351:1359	cancer immune responses	1337:1359	cancer immune responses	1337:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	7	35	theme	antigen-carrying	986:1001	arg1	proteins					1003:1010	Tn antigen-carrying proteins CD43 and CD45	983:1024	Tn antigen-carrying proteins CD43 and CD45	983:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	35	theme	antigen-carrying	986:1001	arg1	CD45					1021:1024	CD45	1021:1024	CD45	1021:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	35	theme	antigen-carrying	986:1001	arg1	CD43					1012:1015	CD43	1012:1015	CD43	1012:1015	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	0	36	theme	Proteins	39:46	arg1	Analysis					15:22	Glycoproteomic Analysis	0:22	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.	0:78	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	9	37	theme	novel	1272:1276	arg1	ligands					1282:1288	novel MGL ligands	1272:1288	novel MGL ligands that presumably act as modulatory molecules in cancer immune responses	1272:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	9	37	theme	novel	1272:1276	arg1	molecules					1324:1332	modulatory molecules	1313:1332	modulatory molecules in cancer immune responses	1313:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	7	38	theme	novel	1070:1074	arg1	ligands					1089:1095	novel cell-surface ligands	1070:1095	novel cell-surface ligands for MGL	1070:1103	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	39	theme	cell-surface	1076:1087	arg1	ligands					1089:1095	novel cell-surface ligands	1070:1095	novel cell-surface ligands for MGL	1070:1103	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	0	40	theme	T-Cell	57:62	arg1	Cells					73:77	Acute T-Cell Leukemia Cells	51:77	Acute T-Cell Leukemia Cells	51:77	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	9	41	theme	MGL	1278:1280	arg1	ligands					1282:1288	novel MGL ligands	1272:1288	novel MGL ligands that presumably act as modulatory molecules in cancer immune responses	1272:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	9	41	theme	MGL	1278:1280	arg1	molecules					1324:1332	modulatory molecules	1313:1332	modulatory molecules in cancer immune responses	1313:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	7	42	dep	proteins	1003:1010	arg1	proteins					1003:1010	Tn antigen-carrying proteins CD43 and CD45	983:1024	Tn antigen-carrying proteins CD43 and CD45	983:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	42	dep	proteins	1003:1010	arg1	CD45					1021:1024	CD45	1021:1024	CD45	1021:1024	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	42	dep	proteins	1003:1010	arg1	CD43					1012:1015	CD43	1012:1015	CD43	1012:1015	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	7	43	theme	MGL	967:969	arg1	ligands					971:977	known MGL ligands	961:977	known MGL ligands	961:977	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	0	44	theme	Acute	51:55	arg1	Cells					73:77	Acute T-Cell Leukemia Cells	51:77	Acute T-Cell Leukemia Cells	51:77	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	4	45	theme	terminal	532:539	arg1	GalNAc					564:569	GalNAc	564:569	GalNAc	564:569	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	4	45	theme	terminal	532:539	arg1	N-acetylgalactosamine					541:561	terminal N-acetylgalactosamine	532:561	terminal N-acetylgalactosamine (GalNAc)	532:570	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	3	46	theme	Other	314:318	arg1	lectin					374:379	the macrophage galactose-type lectin	344:379	the macrophage galactose-type lectin (MGL)	344:385	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	46	theme	Other	314:318	arg1	lectins					327:333	Other C-type lectins	314:333	Other C-type lectins	314:333	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	6	47	theme	glycoproteomic	792:805	arg1	workflow					807:814	a glycoproteomic workflow	790:814	a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line	790:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	2	48	theme	glycan-binding	216:229	arg1	lectins					204:210	Most C-type lectins	192:210	Most C-type lectins	192:210	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	2	48	theme	glycan-binding	216:229	arg1	proteins					231:238	glycan-binding proteins	216:238	glycan-binding proteins	216:238	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	3	49	from	recognition	451:461	arg1	cells					499:503	cancer cells	492:503	cancer cells	492:503	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	50	theme	cancer	492:497	arg1	cells					499:503	cancer cells	492:503	cancer cells	492:503	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	9	51	dep	identification	1240:1253	arg1	the					1236:1238	the	1236:1238	the	1236:1238	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	5	52	theme	glycan	658:663	arg1	specificity					665:675	this glycan specificity	653:675	this glycan specificity of MGL	653:682	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	1	53	theme	proteins	118:125	arg1	proteins					118:125	proteins	118:125	proteins involved in many human physiological and pathological processes	118:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	53	theme	proteins	118:125	arg1	group					109:113	a diverse group	99:113	a diverse group of proteins involved in many human physiological and pathological processes	99:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	1	53	theme	proteins	118:125	arg1	lectins					87:93	C-type lectins	80:93	C-type lectins	80:93	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	6	54	from	ligands	894:900	arg1	line					936:939	the human Jurkat leukemia cell line	905:939	the human Jurkat leukemia cell line	905:939	We present a glycoproteomic workflow for the identification of MGL-binding proteins, which we applied to study MGL ligands on the human Jurkat leukemia cell line.
30582698	2	55	theme	C-type	197:202	arg1	lectins					204:210	Most C-type lectins	192:210	Most C-type lectins	192:210	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	2	55	theme	C-type	197:202	arg1	proteins					231:238	glycan-binding proteins	216:238	glycan-binding proteins	216:238	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	3	56	theme	glycosylation	475:487	arg1	recognition					451:461	the recognition	447:461	the recognition of aberrant glycosylation on cancer cells	447:503	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	3	57	theme	aberrant	466:473	arg1	glycosylation					475:487	aberrant glycosylation	466:487	aberrant glycosylation	466:487	Other C-type lectins, such as the macrophage galactose-type lectin (MGL), have been shown to induce immunosuppressive responses upon the recognition of aberrant glycosylation on cancer cells.
30582698	2	58	theme	Most	192:195	arg1	lectins					204:210	Most C-type lectins	192:210	Most C-type lectins	192:210	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	2	58	theme	Most	192:195	arg1	proteins					231:238	glycan-binding proteins	216:238	glycan-binding proteins	216:238	Most C-type lectins are glycan-binding proteins, some of which are pivotal for innate immune responses against pathogens.
30582698	5	59	gly	glycoproteins	750:762	arg1	glycoproteins					750:762	the actual glycoproteins	739:762	the actual glycoproteins that bind MGL	739:776	Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL.
30582698	9	60	from	molecules	1324:1332	arg1	responses					1351:1359	cancer immune responses	1337:1359	cancer immune responses	1337:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	4	61	theme	malignant	624:632	arg1	cells					634:638	malignant cells	624:638	malignant cells	624:638	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	0	62	theme	Leukemia	64:71	arg1	Cells					73:77	Acute T-Cell Leukemia Cells	51:77	Acute T-Cell Leukemia Cells	51:77	Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells.
30582698	7	63	from	ligands	971:977	arg1	cells					1035:1039	these cells	1029:1039	these cells	1029:1039	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30582698	9	64	theme	modulatory	1313:1322	arg1	ligands					1282:1288	novel MGL ligands	1272:1288	novel MGL ligands that presumably act as modulatory molecules in cancer immune responses	1272:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	9	64	theme	modulatory	1313:1322	arg1	molecules					1324:1332	modulatory molecules	1313:1332	modulatory molecules in cancer immune responses	1313:1359	Altogether, our data provide new insight into the identification and structure of novel MGL ligands that presumably act as modulatory molecules in cancer immune responses.
30582698	4	65	theme	Tn	585:586	arg1	antigen					588:594	the Tn antigen	581:594	the Tn antigen	581:594	MGL is known to recognize terminal N-acetylgalactosamine (GalNAc), such as the Tn antigen, which is commonly found on malignant cells.
30582698	1	66	theme	many	139:142	arg1	processes					181:189	many human physiological and pathological processes	139:189	many human physiological and pathological processes	139:189	C-type lectins are a diverse group of proteins involved in many human physiological and pathological processes.
30582698	7	67	dep	ligands	971:977	arg1	the					957:959	the	957:959	the	957:959	In addition to the known MGL ligands and Tn antigen-carrying proteins CD43 and CD45 on these cells, we have identified a set of novel cell-surface ligands for MGL.
30076915	8	0	from	study	1342:1346	arg1	absence					1284:1290	the absence	1280:1290	the absence of compound-related adverse effects in the 90-day study	1280:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	6	1	theme	direct	1181:1186	arg1	comparison					1188:1197	direct comparison	1181:1197	direct comparison with the high-dose 2'-FL/DFL group	1181:1232	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	1	2	theme	indigestible	166:177	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	2	theme	indigestible	166:177	arg1	carbohydrates					179:191	endogenous indigestible carbohydrates	155:191	endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF)	155:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	6	3	theme	high-dose	1208:1216	arg1	group					1228:1232	the high-dose 2'-FL/DFL group	1204:1232	the high-dose 2'-FL/DFL group	1204:1232	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	7	4	theme	genotoxicity	1250:1261	arg1	evidence					1238:1245	No evidence	1235:1245	No evidence of genotoxicity	1235:1261	No evidence of genotoxicity was observed.
30076915	6	5	theme	IF	1149:1150	arg1	fructooligosaccharide					1153:1173	IF (fructooligosaccharide)	1149:1174	IF (fructooligosaccharide)	1149:1174	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	4	6	theme	Safety	739:744	arg1	assessment					746:755	Safety assessment	739:755	Safety assessment of 2'-FL/DFL	739:768	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	4	7	theme	oral	835:838	arg1	study					849:853	a subchronic oral toxicity study	822:853	a subchronic oral toxicity study	822:853	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	8	8	theme	compound-related	1295:1310	arg1	effects					1320:1326	compound-related adverse effects	1295:1326	compound-related adverse effects in the 90-day study	1295:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	2	9	theme	human	311:315	arg1	breastmilk					317:326	human breastmilk	311:326	human breastmilk	311:326	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	5	10	theme	8:1	892:894	arg1	/DFL					886:889	2'-FL/DFL	881:889	2'-FL/DFL (8:1 ratio)	881:901	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	5	10	theme	8:1	892:894	arg1	ratio					896:900	8:1 ratio	892:900	8:1 ratio	892:900	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	9	11	theme	food	1482:1485	arg1	ingredient					1487:1496	a food ingredient	1480:1496	a food ingredient	1480:1496	These results support the use of 2'-FL/DFL in IF and as a food ingredient.
30076915	4	12	theme	subchronic	824:833	arg1	study					849:853	a subchronic oral toxicity study	822:853	a subchronic oral toxicity study	822:853	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	6	13	theme	reference	1060:1068	arg1	group					1078:1082	A concurrent reference control group	1047:1082	A concurrent reference control group	1047:1082	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	5	14	theme	neonatal	923:930	arg1	rats					932:935	neonatal rats	923:935	neonatal rats	923:935	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	6	15	theme	concurrent	1049:1058	arg1	group					1078:1082	A concurrent reference control group	1047:1082	A concurrent reference control group	1047:1082	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	4	16	theme	in	790:791	arg1	tests					812:816	in vitro genotoxicity tests	790:816	in vitro genotoxicity tests	790:816	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	2	17	theme	major	297:301	arg1	2'-fucosyllactose					332:348	2'-fucosyllactose	332:348	2'-fucosyllactose (2'-FL)	332:356	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	2	17	theme	major	297:301	arg1	HMOs					303:306	Two major HMOs	293:306	Two major HMOs in human breastmilk	293:326	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	8	18	from	absence	1284:1290	arg1	study					1342:1346	the 90-day study	1331:1346	the 90-day study	1331:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	2	19	theme	HMOs	473:476	arg1	versions					461:468	manufactured structurally identical versions	425:468	manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)]	425:533	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	8	20	theme	adverse	1312:1318	arg1	effects					1320:1326	compound-related adverse effects	1295:1326	compound-related adverse effects in the 90-day study	1295:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	4	21	theme	tests	812:816	arg1	conduct					779:785	conduct	779:785	conduct of in vitro genotoxicity tests and a subchronic oral toxicity study	779:853	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	5	22	theme	2'-FL	881:885	arg1	/DFL					886:889	2'-FL/DFL	881:889	2'-FL/DFL (8:1 ratio)	881:901	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	5	22	theme	2'-FL	881:885	arg1	ratio					896:900	8:1 ratio	892:900	8:1 ratio	892:900	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	1	23	theme	largest	210:216	arg1	difference					232:241	the largest compositional difference	206:241	the largest compositional difference between human breastmilk and infant formula (IF)	206:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	0	24	theme	Safety	0:5	arg1	evaluation					7:16	Safety evaluation	0:16	Safety evaluation of a mixture of the human-identical milk	0:57	Safety evaluation of a mixture of the human-identical milk oligosaccharides 2'-fucosyllactose and difucosyllactose.
30076915	4	25	theme	genotoxicity	799:810	arg1	tests					812:816	in vitro genotoxicity tests	790:816	in vitro genotoxicity tests	790:816	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	5	26	theme	body	963:966	arg1	bw					976:977	bw	976:977	bw	976:977	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	5	26	theme	body	963:966	arg1	weight					968:973	5000 mg/kg body weight	952:973	5000 mg/kg body weight (bw)/day	952:982	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	1	27	theme	compositional	218:230	arg1	difference					232:241	the largest compositional difference	206:241	the largest compositional difference between human breastmilk and infant formula (IF)	206:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	2	28	theme	human	574:578	arg1	milk					580:583	human milk	574:583	human milk	574:583	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	1	29	theme	Human	116:120	arg1	HMOs					145:148	HMOs	145:148	HMOs	145:148	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	29	theme	Human	116:120	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	29	theme	Human	116:120	arg1	carbohydrates					179:191	endogenous indigestible carbohydrates	155:191	endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF)	155:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	6	30	with	comparison	1188:1197	arg1	group					1228:1232	the high-dose 2'-FL/DFL group	1204:1232	the high-dose 2'-FL/DFL group	1204:1232	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	2	31	theme	identical	451:459	arg1	versions					461:468	manufactured structurally identical versions	425:468	manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)]	425:533	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	6	32	theme	control	1070:1076	arg1	group					1078:1082	A concurrent reference control group	1047:1082	A concurrent reference control group	1047:1082	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	1	33	theme	milk	122:125	arg1	HMOs					145:148	HMOs	145:148	HMOs	145:148	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	33	theme	milk	122:125	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	33	theme	milk	122:125	arg1	carbohydrates					179:191	endogenous indigestible carbohydrates	155:191	endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF)	155:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	8	34	from	effects	1320:1326	arg1	study					1342:1346	the 90-day study	1331:1346	the 90-day study	1331:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	2	35	theme	manufactured	425:436	arg1	versions					461:468	manufactured structurally identical versions	425:468	manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)]	425:533	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	2	36	theme	milk	504:507	arg1	HiMOs					527:531	HiMOs	527:531	HiMOs	527:531	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	2	36	theme	milk	504:507	arg1	oligosaccharides					509:524	human-identical milk oligosaccharides	488:524	human-identical milk oligosaccharides (HiMOs)]	488:533	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	5	37	dep	administered	907:918	arg1	followed					1009:1016	followed	1009:1016	followed by a 4-week recovery period	1009:1044	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	6	38	theme	/DFL	1223:1226	arg1	group					1228:1232	the high-dose 2'-FL/DFL group	1204:1232	the high-dose 2'-FL/DFL group	1204:1232	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	8	39	theme	90-day	1335:1340	arg1	study					1342:1346	the 90-day study	1331:1346	the 90-day study	1331:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	1	40	theme	human	251:255	arg1	breastmilk					257:266	human breastmilk	251:266	human breastmilk	251:266	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	0	41	theme	mixture	23:29	arg1	evaluation					7:16	Safety evaluation	0:16	Safety evaluation of a mixture of the human-identical milk	0:57	Safety evaluation of a mixture of the human-identical milk oligosaccharides 2'-fucosyllactose and difucosyllactose.
30076915	5	42	theme	4-week	1023:1028	arg1	period					1039:1044	a 4-week recovery period	1021:1044	a 4-week recovery period	1021:1044	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	2	43	theme	human-identical	488:502	arg1	HiMOs					527:531	HiMOs	527:531	HiMOs	527:531	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	2	43	theme	human-identical	488:502	arg1	oligosaccharides					509:524	human-identical milk oligosaccharides	488:524	human-identical milk oligosaccharides (HiMOs)]	488:533	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	4	44	theme	/DFL	765:768	arg1	assessment					746:755	Safety assessment	739:755	Safety assessment of 2'-FL/DFL	739:768	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	0	45	theme	human-identical	38:52	arg1	milk					54:57	the human-identical milk	34:57	the human-identical milk	34:57	Safety evaluation of a mixture of the human-identical milk oligosaccharides 2'-fucosyllactose and difucosyllactose.
30076915	5	46	theme	recovery	1030:1037	arg1	period					1039:1044	a 4-week recovery period	1021:1044	a 4-week recovery period	1021:1044	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	4	47	theme	study	849:853	arg1	conduct					779:785	conduct	779:785	conduct of in vitro genotoxicity tests and a subchronic oral toxicity study	779:853	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	6	48	theme	oligosaccharide	1117:1131	arg1	5000 mg/kg bw/day					1093:1109	5000 mg/kg bw/day	1093:1109	5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide)	1093:1174	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	6	49	theme	2'-FL	1218:1222	arg1	group					1228:1232	the high-dose 2'-FL/DFL group	1204:1232	the high-dose 2'-FL/DFL group	1204:1232	A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group.
30076915	4	50	theme	toxicity	840:847	arg1	study					849:853	a subchronic oral toxicity study	822:853	a subchronic oral toxicity study	822:853	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	3	51	theme	HiMOs	663:667	arg1	mixture					646:652	a mixture	644:652	a mixture of these HiMOs (2'-FL/DFL)	644:679	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	3	51	theme	HiMOs	663:667	arg1	ingredient					727:736	a food ingredient	720:736	a food ingredient	720:736	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	5	52	theme	5000 mg/kg	952:961	arg1	bw					976:977	bw	976:977	bw	976:977	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	5	52	theme	5000 mg/kg	952:961	arg1	weight					968:973	5000 mg/kg body weight	952:973	5000 mg/kg body weight (bw)/day	952:982	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	5	53	theme	subchronic	863:872	arg1	study					874:878	the subchronic study	859:878	the subchronic study	859:878	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	2	54	from	HMOs	303:306	arg1	breastmilk					317:326	human breastmilk	311:326	human breastmilk	311:326	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	3	55	theme	food	722:725	arg1	mixture					646:652	a mixture	644:652	a mixture of these HiMOs (2'-FL/DFL)	644:679	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	3	55	theme	food	722:725	arg1	ingredient					727:736	a food ingredient	720:736	a food ingredient	720:736	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	5	56	theme	weight	968:973	arg1	/day					979:982	5000 mg/kg body weight (bw)/day	952:982	5000 mg/kg body weight (bw)/day	952:982	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
30076915	8	57	theme	effects	1320:1326	arg1	absence					1284:1290	the absence	1280:1290	the absence of compound-related adverse effects in the 90-day study	1280:1346	In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level.
30076915	2	58	theme	milk	580:583	arg1	composition					559:569	the composition	555:569	the composition of human milk	555:583	Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk.
30076915	9	59	theme	2'-FL	1457:1461	arg1	/DFL					1462:1465	2'-FL/DFL	1457:1465	2'-FL/DFL	1457:1465	These results support the use of 2'-FL/DFL in IF and as a food ingredient.
30076915	3	60	theme	human	632:636	arg1	milk					638:641	human milk	632:641	human milk	632:641	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	1	61	theme	infant	272:277	arg1	formula					279:285	infant formula	272:285	infant formula (IF)	272:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	4	62	theme	2'-FL	760:764	arg1	/DFL					765:768	2'-FL/DFL	760:768	2'-FL/DFL	760:768	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	3	63	located	found	614:618	arg2	2'-FL					589:593	2'-FL	589:593	2'-FL	589:593	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	3	63	located	found	614:618	arg1	milk					638:641	human milk	632:641	human milk	632:641	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	3	63	located	found	614:618	arg2	DFL					599:601	DFL	599:601	DFL	599:601	As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient.
30076915	9	64	theme	/DFL	1462:1465	arg1	use					1450:1452	the use	1446:1452	the use of 2'-FL/DFL in IF and as a food ingredient	1446:1496	These results support the use of 2'-FL/DFL in IF and as a food ingredient.
30076915	0	65	theme	milk	54:57	arg1	mixture					23:29	a mixture	21:29	a mixture of the human-identical milk	21:57	Safety evaluation of a mixture of the human-identical milk oligosaccharides 2'-fucosyllactose and difucosyllactose.
30076915	4	66	dep	in	790:791	arg1	vitro					793:797	vitro	793:797	vitro	793:797	Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study.
30076915	1	67	theme	endogenous	155:164	arg1	oligosaccharides					127:142	Human milk oligosaccharides	116:142	Human milk oligosaccharides (HMOs)	116:149	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	1	67	theme	endogenous	155:164	arg1	carbohydrates					179:191	endogenous indigestible carbohydrates	155:191	endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF)	155:290	Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF).
30076915	5	68	dep	/day	979:982	arg1	up					946:947	up	946:947	up	946:947	In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period.
31326513	6	0	theme	interactions	879:890	arg1	presence					851:858	The presence	847:858	The presence of HA and possible interactions between CS and HA	847:908	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	12	1	theme	cell	1411:1414	arg1	proliferation					1416:1428	cell proliferation	1411:1428	cell proliferation	1411:1428	However, cell proliferation and adhesion were more when HA was coated through a direct simultaneous method.
31326513	6	2	theme	HA	863:864	arg1	presence					851:858	The presence	847:858	The presence of HA and possible interactions between CS and HA	847:908	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	6	3	theme	FTIR	960:963	arg1	analysis					989:996	FTIR and thermal gravimetric analysis	960:996	analysis	989:996	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	6	4	theme	possible	870:877	arg1	interactions					879:890	possible interactions	870:890	possible interactions between CS and HA	870:908	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	5	5	dep	remarkable	776:785	arg1	morphological					787:799	morphological	787:799	morphological	787:799	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	12	6	theme	simultaneous	1489:1500	arg1	method					1502:1507	a direct simultaneous method	1480:1507	a direct simultaneous method	1480:1507	However, cell proliferation and adhesion were more when HA was coated through a direct simultaneous method.
31326513	1	7	theme	tissue	258:263	arg1	applications					277:288	tissue engineering applications	258:288	tissue engineering applications	258:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	4	8	theme	electrospun	489:499	arg1	nanofiner					501:509	electrospun nanofiner	489:509	electrospun nanofiner	489:509	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	1	9	theme	engineering	265:275	arg1	applications					277:288	tissue engineering applications	258:288	tissue engineering applications	258:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	4	10	dep	done	554:557	arg1	HA/CS2					659:664	HA/CS2	659:664	HA/CS2	659:664	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	4	10	dep	done	554:557	arg1	HA/CS1					560:565	HA/CS1	560:565	HA/CS1	560:565	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	6	11	theme	coated	939:944	arg1	nanofibers					946:955	both HA coated nanofibers	931:955	both HA coated nanofibers	931:955	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	8	12	theme	fibers	1152:1157	arg1	wettability					1127:1137	the wettability	1123:1137	the wettability of resultant fibers	1123:1157	Contact angel measurement revealed differences in the wettability of resultant fibers.
31326513	5	13	theme	mats	705:708	arg1	structure					688:696	The overall fibrous structure	668:696	The overall fibrous structure of the mats	668:708	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	4	14	theme	first	475:479	arg1	method					481:486	the first method	471:486	the first method	471:486	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	5	15	theme	HA/CS2	831:836	arg1	samples					838:844	HA/CS2 samples	831:844	HA/CS2 samples	831:844	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	9	16	theme	high	1191:1194	arg1	wettability					1196:1206	high wettability	1191:1206	high wettability	1191:1206	Although both scaffolds showed high wettability, the HA/CS2 had lower wettability than HA/CS1.
31326513	1	17	theme	charged	169:175	arg1	Chitosan					111:118	Chitosan	111:118	Chitosan (CS)	111:123	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	17	theme	charged	169:175	arg1	acid					140:143	hyaluronic acid	129:143	hyaluronic acid (HA)	129:148	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	17	theme	charged	169:175	arg1	polysaccharides					185:199	two oppositely charged natural polysaccharides	154:199	two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications	154:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	0	18	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid coated electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating.
31326513	11	19	theme	cell	1382:1385	arg1	proliferation					1387:1399	cell proliferation	1382:1399	cell proliferation	1382:1399	Both HA coated scaffolds showed improvement in cell proliferation.
31326513	1	20	theme	natural	177:183	arg1	Chitosan					111:118	Chitosan	111:118	Chitosan (CS)	111:123	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	20	theme	natural	177:183	arg1	acid					140:143	hyaluronic acid	129:143	hyaluronic acid (HA)	129:148	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	20	theme	natural	177:183	arg1	polysaccharides					185:199	two oppositely charged natural polysaccharides	154:199	two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications	154:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	0	21	theme	electrospun	23:33	arg1	nanofibers					50:59	electrospun chitosan-based nanofibers	23:59	electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating	23:108	Hyaluronic acid coated electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating.
31326513	7	22	theme	more	1036:1039	arg1	interactions					1041:1052	more interactions	1036:1052	more interactions between HA and CS	1036:1070	However, HA/CS2 nanofibers indicated more interactions between HA and CS.
31326513	2	23	theme	composite	309:317	arg1	fibers					319:324	composite fibers	309:324	composite fibers composed of CS and HA by electrospinning and subsequent coating	309:388	Here, we prepared composite fibers composed of CS and HA by electrospinning and subsequent coating.
31326513	5	24	theme	remarkable	776:785	arg1	difference					801:810	no remarkable morphological difference	773:810	no remarkable morphological difference between HA/CS1 and HA/CS2 samples	773:844	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	6	25	theme	thermal	969:975	arg1	analysis					989:996	FTIR and thermal gravimetric analysis	960:996	analysis	989:996	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	12	26	theme	direct	1482:1487	arg1	method					1502:1507	a direct simultaneous method	1480:1507	a direct simultaneous method	1480:1507	However, cell proliferation and adhesion were more when HA was coated through a direct simultaneous method.
31326513	5	27	theme	overall	672:678	arg1	structure					688:696	The overall fibrous structure	668:696	The overall fibrous structure of the mats	668:708	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	3	28	theme	CS	447:448	arg1	nanofibers					450:459	CS nanofibers	447:459	CS nanofibers	447:459	In this regard, two approaches were applied for coating CS nanofibers by HA.
31326513	0	29	theme	chitosan-based	35:48	arg1	nanofibers					50:59	electrospun chitosan-based nanofibers	23:59	electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating	23:108	Hyaluronic acid coated electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating.
31326513	5	30	theme	fibrous	680:686	arg1	structure					688:696	The overall fibrous structure	668:696	The overall fibrous structure of the mats	668:708	The overall fibrous structure of the mats was preserved after coating through both methods and there was no remarkable morphological difference between HA/CS1 and HA/CS2 samples.
31326513	8	31	from	differences	1108:1118	arg1	wettability					1127:1137	the wettability	1123:1137	the wettability of resultant fibers	1123:1157	Contact angel measurement revealed differences in the wettability of resultant fibers.
31326513	4	32	theme	second	582:587	arg1	approach					589:596	the second approach	578:596	the second approach	578:596	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	10	33	theme	scaffolds	1324:1332	arg1	cytocompatibility					1299:1315	cytocompatibility	1299:1315	cytocompatibility of the scaffolds	1299:1332	More importantly, there was a difference in cytocompatibility of the scaffolds.
31326513	8	34	theme	resultant	1142:1150	arg1	fibers					1152:1157	resultant fibers	1142:1157	resultant fibers	1142:1157	Contact angel measurement revealed differences in the wettability of resultant fibers.
31326513	8	35	theme	Contact	1073:1079	arg1	measurement					1087:1097	Contact angel measurement	1073:1097	Contact angel measurement	1073:1097	Contact angel measurement revealed differences in the wettability of resultant fibers.
31326513	9	36	theme	lower	1224:1228	arg1	wettability					1230:1240	lower wettability	1224:1240	lower wettability	1224:1240	Although both scaffolds showed high wettability, the HA/CS2 had lower wettability than HA/CS1.
31326513	4	37	dep	coating	542:548	arg1	done					554:557	done	554:557	was done	550:557	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	7	38	theme	HA/CS2	1008:1013	arg1	nanofibers					1015:1024	HA/CS2 nanofibers	1008:1024	HA/CS2 nanofibers	1008:1024	However, HA/CS2 nanofibers indicated more interactions between HA and CS.
31326513	1	39	theme	hyaluronic	129:138	arg1	HA					146:147	HA	146:147	HA	146:147	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	39	theme	hyaluronic	129:138	arg1	acid					140:143	hyaluronic acid	129:143	hyaluronic acid (HA)	129:148	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	39	theme	hyaluronic	129:138	arg1	polysaccharides					185:199	two oppositely charged natural polysaccharides	154:199	two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications	154:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	39	theme	hyaluronic	129:138	arg1	Chitosan					111:118	Chitosan	111:118	Chitosan (CS)	111:123	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	9	40	contain	had	1220:1222	arg1	HA/CS2					1213:1218	the HA/CS2	1209:1218	the HA/CS2	1209:1218	Although both scaffolds showed high wettability, the HA/CS2 had lower wettability than HA/CS1.
31326513	9	40	contain	had	1220:1222	arg2	wettability					1230:1240	lower wettability	1224:1240	lower wettability	1224:1240	Although both scaffolds showed high wettability, the HA/CS2 had lower wettability than HA/CS1.
31326513	8	41	theme	angel	1081:1085	arg1	measurement					1087:1097	Contact angel measurement	1073:1097	Contact angel measurement	1073:1097	Contact angel measurement revealed differences in the wettability of resultant fibers.
31326513	11	42	from	improvement	1367:1377	arg1	proliferation					1387:1399	cell proliferation	1382:1399	cell proliferation	1382:1399	Both HA coated scaffolds showed improvement in cell proliferation.
31326513	4	43	dep	carried	631:637	arg1	out					639:641	out	639:641	out	639:641	In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2).
31326513	11	44	theme	coated	1343:1348	arg1	scaffolds					1350:1358	Both HA coated scaffolds	1335:1358	Both HA coated scaffolds	1335:1358	Both HA coated scaffolds showed improvement in cell proliferation.
31326513	6	45	theme	gravimetric	977:987	arg1	analysis					989:996	FTIR and thermal gravimetric analysis	960:996	analysis	989:996	The presence of HA and possible interactions between CS and HA were demonstrated in both HA coated nanofibers by FTIR and thermal gravimetric analysis.
31326513	2	46	theme	subsequent	371:380	arg1	coating					382:388	subsequent coating	371:388	subsequent coating	371:388	Here, we prepared composite fibers composed of CS and HA by electrospinning and subsequent coating.
31326513	1	47	theme	nanofibrous	232:242	arg1	scaffolds					244:252	nanofibrous scaffolds	232:252	nanofibrous scaffolds	232:252	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
31326513	1	48	theme	scaffolds	244:252	arg1	preparation					217:227	preparation	217:227	preparation of nanofibrous scaffolds for tissue engineering applications	217:288	Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications.
30986057	8	0	theme	Increasing	985:994	arg1	knowledge					996:1004	Increasing knowledge	985:1004	Increasing knowledge in this regard	985:1019	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	0	1	theme	Features	76:83	arg1	Effect					51:56	Effect	51:56	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.	0:116	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	6	2	theme	β-galactosyl	739:750	arg1	fructoses					752:760	β-galactosyl fructoses	739:760	β-galactosyl fructoses	739:760	This was also observed for β-galactosyl fructoses and β-galactosyl glucoses, where the presence of fructose provided higher resistance to digestion.
30986057	7	3	theme	prebiotics	973:982	arg1	composition					958:968	composition	958:968	composition	958:968	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	7	3	theme	prebiotics	973:982	arg1	structure					944:952	structure	944:952	structure	944:952	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	8	4	theme	mixtures	1069:1076	arg1	synthesis					1052:1060	the future synthesis	1041:1060	the future synthesis of new mixtures of carbohydrates	1041:1093	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	8	5	theme	specific	1176:1183	arg1	properties					1185:1194	specific properties	1176:1194	specific properties	1176:1194	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	0	6	theme	Intestinal	94:103	arg1	Degradation					105:115	Their Intestinal Degradation	88:115	Their Intestinal Degradation	88:115	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	1	7	from	pig	171:173	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	6	8	theme	higher	829:834	arg1	resistance					836:845	higher resistance	829:845	higher resistance to digestion	829:858	This was also observed for β-galactosyl fructoses and β-galactosyl glucoses, where the presence of fructose provided higher resistance to digestion.
30986057	2	9	theme	hydrolysis	276:285	arg1	rates					287:291	Dissimilar hydrolysis rates	265:291	Dissimilar hydrolysis rates	265:291	Dissimilar hydrolysis rates were detected after digestion.
30986057	0	10	from	Effect	51:56	arg1	Degradation					105:115	Their Intestinal Degradation	88:115	Their Intestinal Degradation	88:115	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	2	11	theme	Dissimilar	265:274	arg1	rates					287:291	Dissimilar hydrolysis rates	265:291	Dissimilar hydrolysis rates	265:291	Dissimilar hydrolysis rates were detected after digestion.
30986057	3	12	theme	monomeric	360:368	arg1	composition					370:380	monomeric composition	360:380	monomeric composition	360:380	Predominant glycosidic linkages and monomeric composition affected the resistance to intestinal digestive enzymes.
30986057	3	13	theme	intestinal	409:418	arg1	enzymes					430:436	intestinal digestive enzymes	409:436	intestinal digestive enzymes	409:436	Predominant glycosidic linkages and monomeric composition affected the resistance to intestinal digestive enzymes.
30986057	8	14	theme	carbohydrates	1081:1093	arg1	mixtures					1069:1076	new mixtures	1065:1076	new mixtures of carbohydrates	1065:1093	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	3	15	theme	Predominant	324:334	arg1	linkages					347:354	Predominant glycosidic linkages	324:354	Predominant glycosidic linkages	324:354	Predominant glycosidic linkages and monomeric composition affected the resistance to intestinal digestive enzymes.
30986057	7	16	theme	intestinal	891:900	arg1	enzymes					912:918	small intestinal digestive enzymes	885:918	small intestinal digestive enzymes	885:918	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	3	17	theme	glycosidic	336:345	arg1	linkages					347:354	Predominant glycosidic linkages	324:354	Predominant glycosidic linkages	324:354	Predominant glycosidic linkages and monomeric composition affected the resistance to intestinal digestive enzymes.
30986057	0	18	theme	In	0:1	arg1	Digestibility					9:21	In Vitro Digestibility	0:21	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.	0:116	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	6	19	theme	fructose	811:818	arg1	presence					799:806	the presence	795:806	the presence of fructose	795:818	This was also observed for β-galactosyl fructoses and β-galactosyl glucoses, where the presence of fructose provided higher resistance to digestion.
30986057	8	20	from	knowledge	996:1004	arg1	regard					1014:1019	this regard	1009:1019	this regard	1009:1019	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	5	21	theme	β	692:692	arg1	GOS					699:701	β(1→6) GOS	692:701	β(1→6) GOS (27.1%)	692:709	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	21	theme	β	692:692	arg1	%					708:708	27.1%	704:708	27.1%	704:708	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	22	theme	Monomeric	606:614	arg1	composition					616:626	Monomeric composition	606:626	Monomeric composition	606:626	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	8	23	theme	probiotic	1231:1239	arg1	species					1241:1247	specific probiotic species	1222:1247	specific probiotic species	1222:1247	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	5	24	theme	1→6	694:696	arg1	GOS					699:701	β(1→6) GOS	692:701	β(1→6) GOS (27.1%)	692:709	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	24	theme	1→6	694:696	arg1	%					708:708	27.1%	704:708	27.1%	704:708	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	8	25	theme	future	1045:1050	arg1	synthesis					1052:1060	the future synthesis	1041:1060	the future synthesis of new mixtures of carbohydrates	1041:1093	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	4	26	dep	susceptible	475:485	arg1	mixture					454:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	26	dep	susceptible	475:485	arg1	%					506:506	50.2%	502:506	50.2%	502:506	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	26	dep	susceptible	475:485	arg1	followed					510:517	followed	510:517	followed by β(1→4) (34.9%)	510:535	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	26	dep	susceptible	475:485	arg1	whereas					538:544	whereas	538:544	whereas	538:544	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	26	dep	susceptible	475:485	arg1	susceptible					475:485	susceptible	475:485	susceptible	475:485	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	8	27	theme	specific	1222:1229	arg1	species					1241:1247	specific probiotic species	1222:1247	specific probiotic species	1222:1247	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	8	28	theme	new	1065:1067	arg1	mixtures					1069:1076	new mixtures	1065:1076	new mixtures of carbohydrates	1065:1093	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	1	29	from	lactulose	247:255	arg1	galactooligosaccharides					195:217	galactooligosaccharides	195:217	galactooligosaccharides from lactose (GOS) and from lactulose (OsLu)	195:262	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	4	30	theme	GOS	450:452	arg1	mixture					454:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	30	theme	GOS	450:452	arg1	%					506:506	50.2%	502:506	50.2%	502:506	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	30	theme	GOS	450:452	arg1	susceptible					475:485	susceptible	475:485	susceptible	475:485	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	30	theme	GOS	450:452	arg1	whereas					538:544	whereas	538:544	whereas	538:544	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	30	theme	GOS	450:452	arg1	followed					510:517	followed	510:517	followed by β(1→4) (34.9%)	510:535	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	0	31	theme	Galactooligosaccharides	26:48	arg1	Digestibility					9:21	In Vitro Digestibility	0:21	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.	0:116	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	7	32	theme	digestive	902:910	arg1	enzymes					912:918	small intestinal digestive enzymes	885:918	small intestinal digestive enzymes	885:918	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	1	33	from	lactose	224:230	arg1	galactooligosaccharides					195:217	galactooligosaccharides	195:217	galactooligosaccharides from lactose (GOS) and from lactulose (OsLu)	195:262	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	5	34	theme	β	660:660	arg1	%					677:677	22.8%	673:677	22.8%	673:677	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	34	theme	β	660:660	arg1	OsLu					667:670	β(1→6) OsLu	660:670	β(1→6) OsLu (22.8%)	660:678	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	3	35	theme	digestive	420:428	arg1	enzymes					430:436	intestinal digestive enzymes	409:436	intestinal digestive enzymes	409:436	Predominant glycosidic linkages and monomeric composition affected the resistance to intestinal digestive enzymes.
30986057	5	36	theme	better	639:644	arg1	resistance					646:655	a better resistance	637:655	a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%)	637:709	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	37	from	resistance	646:655	arg1	%					677:677	22.8%	673:677	22.8%	673:677	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	37	from	resistance	646:655	arg1	OsLu					667:670	β(1→6) OsLu	660:670	β(1→6) OsLu (22.8%)	660:678	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	1	38	theme	Small	118:122	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	7	39	dep	structure	944:952	arg1	the					940:942	the	940:942	the	940:942	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	7	40	theme	small	885:889	arg1	enzymes					912:918	small intestinal digestive enzymes	885:918	small intestinal digestive enzymes	885:918	Thus, the resistance to small intestinal digestive enzymes highly depends upon the structure and composition of prebiotics.
30986057	1	41	theme	intestinal	124:133	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	6	42	theme	β-galactosyl	766:777	arg1	glucoses					779:786	β-galactosyl glucoses	766:786	β-galactosyl glucoses	766:786	This was also observed for β-galactosyl fructoses and β-galactosyl glucoses, where the presence of fructose provided higher resistance to digestion.
30986057	4	43	theme	β	443:443	arg1	mixture					454:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture	439:460	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	43	theme	β	443:443	arg1	%					506:506	50.2%	502:506	50.2%	502:506	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	43	theme	β	443:443	arg1	susceptible					475:485	susceptible	475:485	susceptible	475:485	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	43	theme	β	443:443	arg1	whereas					538:544	whereas	538:544	whereas	538:544	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	4	43	theme	β	443:443	arg1	followed					510:517	followed	510:517	followed by β(1→4) (34.9%)	510:535	The β(1→3) GOS mixture was the most susceptible to hydrolysis (50.2%), followed by β(1→4) (34.9%), whereas β(1→6) linkages were highly resistant to digestion (27.1%).
30986057	1	44	theme	brush	135:139	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	0	45	dep	Digestibility	9:21	arg1	Effect					51:56	Effect	51:56	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.	0:116	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	1	46	theme	border	141:146	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	8	47	theme	tailored	1151:1158	arg1	prebiotics					1160:1169	tailored prebiotics	1151:1169	tailored prebiotics with specific properties	1151:1194	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	0	48	theme	Structural	65:74	arg1	Features					76:83	the Structural Features	61:83	the Structural Features	61:83	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30986057	8	49	with	prebiotics	1160:1169	arg1	properties					1185:1194	specific properties	1176:1194	specific properties	1176:1194	Increasing knowledge in this regard could contribute to the future synthesis of new mixtures of carbohydrates, highly resistant to digestion and with potential to be tailored prebiotics with specific properties, targeting, for instance, specific probiotic species.
30986057	1	50	used	used	180:183	arg2	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	1	51	theme	membrane	148:155	arg1	vesicles					157:164	Small intestinal brush border membrane vesicles	118:164	Small intestinal brush border membrane vesicles from pig	118:173	Small intestinal brush border membrane vesicles from pig were used to digest galactooligosaccharides from lactose (GOS) and from lactulose (OsLu).
30986057	5	52	theme	1→6	662:664	arg1	%					677:677	22.8%	673:677	22.8%	673:677	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	5	52	theme	1→6	662:664	arg1	OsLu					667:670	β(1→6) OsLu	660:670	β(1→6) OsLu (22.8%)	660:678	Monomeric composition provided a better resistance in β(1→6) OsLu (22.8%) compared to β(1→6) GOS (27.1%).
30986057	0	53	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation.
30110252	0	0	theme	gum	75:77	arg1	products					83:90	their bean gum by- products	64:90	their bean gum by- products	64:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	5	1	theme	dry	1006:1008	arg1	weight					1010:1015	dry weight	1006:1015	dry weight	1006:1015	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	2	2	theme	production	306:315	arg1	LBG					334:336	LBG	334:336	LBG	334:336	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	2	theme	production	306:315	arg1	gum					329:331	the production locust bean gum	302:331	the production locust bean gum (LBG) of seeds grown in Algeria	302:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	0	3	theme	bean	70:73	arg1	products					83:90	their bean gum by- products	64:90	their bean gum by- products	64:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	5	4	dep	Results	772:778	arg1	acetonicextract					788:802	Aqueous acetonicextract	780:802	Results Aqueous acetonicextract of carob seeds	772:817	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	5	5	theme	carob	807:811	arg1	seeds					813:817	carob seeds	807:817	carob seeds	807:817	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	0	6	theme	products	83:90	arg1	activity					12:19	Antioxidant activity	0:19	Antioxidant activity of carob seeds	0:34	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	0	6	theme	products	83:90	arg1	composition					49:59	chemical composition	40:59	chemical composition of their bean gum by- products	40:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	3	7	theme	condensed	460:468	arg1	tannins					470:476	condensed tannins	460:476	condensed tannins	460:476	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	0	8	theme	by-	79:81	arg1	products					83:90	their bean gum by- products	64:90	their bean gum by- products	64:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	5	9	theme	total	865:869	arg1	flavonoids					871:880	total flavonoids	865:880	total flavonoids	865:880	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	3	10	theme	total	427:431	arg1	tannins					433:439	total tannins	427:439	total tannins	427:439	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	11	theme	total	410:414	arg1	phenolics					416:424	total phenolics	410:424	total phenolics	410:424	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	12	theme	seeds	621:625	arg1	extracts					572:579	the aqueous methanolic, ethanolic and acetonic extracts	525:579	the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	525:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	5	13	theme	flavonoids	871:880	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	6	14	theme	same	1036:1039	arg1	extract					1041:1047	The same extract	1032:1047	The same extract	1032:1047	The same extract showed also the best antioxidant activity in all assays compared to other extracts.
30110252	2	15	theme	present	220:226	arg1	study					228:232	this present study	215:232	this present study	215:232	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	1	16	theme	old	145:147	arg1	fruit					156:160	very old edible fruit	140:160	very old edible fruit	140:160	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	3	17	theme	total	442:446	arg1	flavonoids					448:457	total flavonoids	442:457	total flavonoids	442:457	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	6	18	theme	other	1117:1121	arg1	extracts					1123:1130	other extracts	1117:1130	other extracts	1117:1130	The same extract showed also the best antioxidant activity in all assays compared to other extracts.
30110252	1	19	theme	edible	149:154	arg1	fruit					156:160	very old edible fruit	140:160	very old edible fruit	140:160	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	0	20	theme	Antioxidant	0:10	arg1	activity					12:19	Antioxidant activity	0:19	Antioxidant activity of carob seeds	0:34	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	4	21	theme	chemical	736:743	arg1	composition					745:755	its chemical composition	732:755	its chemical composition	732:755	Furthermore, the carob bean gum from seeds was extracted and its chemical composition was evaluated.
30110252	2	22	theme	compounds	262:270	arg1	composition					238:248	the composition	234:248	the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria	234:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	23	theme	gum	329:331	arg1	composition					238:248	the composition	234:248	the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria	234:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	0	24	theme	carob	24:28	arg1	seeds					30:34	carob seeds	24:34	carob seeds	24:34	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	3	25	theme	colorimetric	649:660	arg1	methods					662:668	colorimetric methods	649:668	colorimetric methods	649:668	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	5	26	theme	highest	828:834	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	2	27	theme	phenolic	253:260	arg1	compounds					262:270	phenolic compounds	253:270	phenolic compounds	253:270	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	3	28	theme	phenolic	391:398	arg1	contents					400:407	The phenolic contents	387:407	The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols)	387:491	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	4	29	theme	bean	694:697	arg1	gum					699:701	the carob bean gum	684:701	the carob bean gum from seeds	684:712	Furthermore, the carob bean gum from seeds was extracted and its chemical composition was evaluated.
30110252	3	30	theme	acetonic	563:570	arg1	extracts					572:579	the aqueous methanolic, ethanolic and acetonic extracts	525:579	the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	525:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	5	31	theme	phenolics	854:862	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	1	32	theme	siliqua	125:131	arg1	L.					133:134	Ceratonia siliqua L.	115:134	Ceratonia siliqua L.	115:134	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	1	32	theme	siliqua	125:131	arg1	carob					108:112	The carob	104:112	The carob (Ceratonia siliqua L.)	104:135	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	5	33	theme	tannins	900:906	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	3	34	theme	extracts	572:579	arg1	activity					513:520	the antioxidant activity	497:520	the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	497:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	34	theme	extracts	572:579	arg1	Methods					379:385	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	379:625	Methods	379:385	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	2	35	theme	seeds	342:346	arg1	activity					289:296	the antioxidant activity	273:296	the antioxidant activity	273:296	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	35	theme	seeds	342:346	arg1	LBG					334:336	LBG	334:336	LBG	334:336	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	35	theme	seeds	342:346	arg1	gum					329:331	the production locust bean gum	302:331	the production locust bean gum (LBG) of seeds grown in Algeria	302:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	35	theme	seeds	342:346	arg1	compounds					262:270	phenolic compounds	253:270	phenolic compounds	253:270	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	0	36	theme	seeds	30:34	arg1	activity					12:19	Antioxidant activity	0:19	Antioxidant activity of carob seeds	0:34	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	0	36	theme	seeds	30:34	arg1	composition					49:59	chemical composition	40:59	chemical composition of their bean gum by- products	40:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	3	37	theme	ethanolic	549:557	arg1	extracts					572:579	the aqueous methanolic, ethanolic and acetonic extracts	525:579	the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	525:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	5	38	theme	condensed	912:920	arg1	tannins					922:928	condensed tannins	912:928	condensed tannins	912:928	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	5	39	theme	Aqueous	780:786	arg1	acetonicextract					788:802	Aqueous acetonicextract	780:802	Results Aqueous acetonicextract of carob seeds	772:817	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	1	40	dep	Background	93:102	arg1	flavorful					163:171	flavorful	163:171	flavorful	163:171	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	1	40	dep	Background	93:102	arg1	used					183:186	used	183:186	often used in foods and beverages	177:209	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	5	41	theme	tannins	922:928	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	5	42	theme	seeds	813:817	arg1	acetonicextract					788:802	Aqueous acetonicextract	780:802	Results Aqueous acetonicextract of carob seeds	772:817	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	0	43	theme	chemical	40:47	arg1	composition					49:59	chemical composition	40:59	chemical composition of their bean gum by- products	40:90	Antioxidant activity of carob seeds and chemical composition of their bean gum by- products.
30110252	6	44	theme	best	1065:1068	arg1	activity					1082:1089	the best antioxidant activity	1061:1089	the best antioxidant activity	1061:1089	The same extract showed also the best antioxidant activity in all assays compared to other extracts.
30110252	3	45	theme	antioxidant	501:511	arg1	activity					513:520	the antioxidant activity	497:520	the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	497:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	4	46	from	seeds	708:712	arg1	gum					699:701	the carob bean gum	684:701	the carob bean gum from seeds	684:712	Furthermore, the carob bean gum from seeds was extracted and its chemical composition was evaluated.
30110252	2	47	theme	activity	289:296	arg1	composition					238:248	the composition	234:248	the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria	234:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	3	48	theme	aqueous	529:535	arg1	extracts					572:579	the aqueous methanolic, ethanolic and acetonic extracts	525:579	the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	525:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	49	dep	Methods	379:385	arg1	contents					400:407	The phenolic contents	387:407	The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols)	387:491	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	50	theme	methanolic	537:546	arg1	extracts					572:579	the aqueous methanolic, ethanolic and acetonic extracts	525:579	the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds	525:625	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	51	dep	contents	400:407	arg1	phenolics					416:424	total phenolics	410:424	total phenolics	410:424	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	51	dep	contents	400:407	arg1	flavonols					482:490	flavonols	482:490	flavonols	482:490	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	51	dep	contents	400:407	arg1	flavonoids					448:457	total flavonoids	442:457	total flavonoids	442:457	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	51	dep	contents	400:407	arg1	tannins					470:476	condensed tannins	460:476	condensed tannins	460:476	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	51	dep	contents	400:407	arg1	tannins					433:439	total tannins	427:439	total tannins	427:439	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	2	52	theme	antioxidant	277:287	arg1	activity					289:296	the antioxidant activity	273:296	the antioxidant activity	273:296	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	5	53	theme	total	848:852	arg1	phenolics					854:862	total phenolics	848:862	total phenolics	848:862	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	6	54	theme	antioxidant	1070:1080	arg1	activity					1082:1089	the best antioxidant activity	1061:1089	the best antioxidant activity	1061:1089	The same extract showed also the best antioxidant activity in all assays compared to other extracts.
30110252	1	55	theme	Ceratonia	115:123	arg1	L.					133:134	Ceratonia siliqua L.	115:134	Ceratonia siliqua L.	115:134	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	1	55	theme	Ceratonia	115:123	arg1	carob					108:112	The carob	104:112	The carob (Ceratonia siliqua L.)	104:135	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	4	56	theme	carob	688:692	arg1	gum					699:701	the carob bean gum	684:701	the carob bean gum from seeds	684:712	Furthermore, the carob bean gum from seeds was extracted and its chemical composition was evaluated.
30110252	1	57	used	used	183:186	arg2	L.					133:134	Ceratonia siliqua L.	115:134	Ceratonia siliqua L.	115:134	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	1	57	used	used	183:186	arg2	carob					108:112	The carob	104:112	The carob (Ceratonia siliqua L.)	104:135	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	5	58	theme	total	894:898	arg1	tannins					900:906	total tannins	894:906	total tannins	894:906	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	2	59	theme	bean	324:327	arg1	LBG					334:336	LBG	334:336	LBG	334:336	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	59	theme	bean	324:327	arg1	gum					329:331	the production locust bean gum	302:331	the production locust bean gum (LBG) of seeds grown in Algeria	302:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	5	60	theme	flavonols	883:891	arg1	contents					836:843	the highest contents	824:843	the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively	824:1029	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	5	61	theme	weight	1010:1015	arg1	0.53±0.01 mg/g					988:1001	0.53±0.01 mg/g	988:1001	0.53±0.01 mg/g of dry weight	988:1015	Results Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively.
30110252	1	62	dep	flavorful	163:171	arg1	fruit					156:160	very old edible fruit	140:160	very old edible fruit	140:160	Background The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages.
30110252	2	63	theme	locust	317:322	arg1	LBG					334:336	LBG	334:336	LBG	334:336	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	2	63	theme	locust	317:322	arg1	gum					329:331	the production locust bean gum	302:331	the production locust bean gum (LBG) of seeds grown in Algeria	302:363	In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
30110252	3	64	dep	extracts	572:579	arg1	70					582:583	70	582:583	70	582:583	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	64	dep	extracts	572:579	arg1	80v/v					593:597	80v/v	593:597	80v/v	593:597	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30110252	3	64	dep	extracts	572:579	arg1	80					586:587	80	586:587	80	586:587	Methods The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods.
30070706	5	0	theme	phase	611:615	arg1	end					599:601	the end	595:601	the end of each phase	595:615	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	1	theme	species	950:956	arg1	proportions					922:932	proportions	922:932	proportions	922:932	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	1	theme	species	950:956	arg1	levels					911:916	levels	911:916	levels	911:916	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	8	2	with	comparison	1462:1471	arg1	p <					1497:1499	p < 0.05	1497:1504	p < 0.05	1497:1504	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	2	with	comparison	1462:1471	arg1	group					1490:1494	the control group	1478:1494	the control group (p < 0.05)	1478:1505	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	5	3	theme	soluble	727:733	arg1	analysis					662:669	biochemical analysis	650:669	biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS)	650:834	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	8	4	theme	red	1371:1373	arg1	species					1383:1389	red complex species	1371:1389	red complex species	1371:1389	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	2	5	theme	palatal	379:385	arg1	appliance					387:395	a palatal appliance	377:395	a palatal appliance containing titanium specimens	377:425	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	2	6	dep	7 days	364:369	arg1	each					371:374	each	371:374	each	371:374	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	1	7	theme	dental	142:147	arg1	surfaces					149:156	dental surfaces	142:156	dental surfaces	142:156	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	8	8	theme	complex	1442:1448	arg1	species					1450:1456	the host-compatible green complex species	1416:1456	the host-compatible green complex species	1416:1456	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	7	9	dep	RESULTS	1079:1085	arg1	found					1134:1138	found	1134:1138	was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05)	1130:1223	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	0	10	from	Effect	0:5	arg1	biofilm					21:27	biofilm	21:27	biofilm	21:27	Effect of sucrose on biofilm formed in situ on titanium material.
30070706	1	11	from	formation	249:257	arg1	surface					271:277	titanium surface	262:277	titanium surface	262:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	3	12	theme	20	487:488	arg1	%					489:489	%	489:489	%	489:489	In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day.
30070706	5	13	theme	extracellular	766:778	arg1	IPS					831:833	IPS	831:833	IPS	831:833	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	13	theme	extracellular	766:778	arg1	polysaccharides					780:794	insoluble (I-EPS) extracellular polysaccharides	748:794	insoluble (I-EPS) extracellular polysaccharides	748:794	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	4	14	dep	rendered	577:584	arg1	0×					587:588	0×	587:588	0×	587:588	As control, no treatment was rendered (0×).
30070706	7	15	dep	exposure	1154:1161	arg1	4×					1168:1169	4×	1168:1169	4×	1168:1169	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	7	15	dep	exposure	1154:1161	arg1	8×					1176:1177	8×	1176:1177	8×	1176:1177	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	7	15	dep	exposure	1154:1161	arg1	0×					1164:1165	0×	1164:1165	0×	1164:1165	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	9	16	contain	has	1589:1591	arg1	exposure					1580:1587	daily sucrose exposure	1566:1587	daily sucrose exposure	1566:1587	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	9	16	contain	has	1589:1591	arg2	effect					1603:1608	a harmful effect	1593:1608	a harmful effect	1593:1608	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	5	17	theme	polysaccharides	780:794	arg1	analysis					662:669	biochemical analysis	650:669	biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS)	650:834	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	2	18	dep	blind	296:300	arg1	in					314:315	in	314:315	in	314:315	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	2	18	dep	blind	296:300	arg1	crossover					303:311	crossover	303:311	crossover	303:311	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	8	19	theme	complex	1375:1381	arg1	species					1383:1389	red complex species	1371:1389	red complex species	1371:1389	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	5	20	theme	wet	682:684	arg1	weight					686:691	biofilm wet weight	674:691	biofilm wet weight (biomass)	674:701	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	20	theme	wet	682:684	arg1	biomass					694:700	biomass	694:700	biomass	694:700	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	6	21	theme	linear	994:999	arg1	regression					1001:1010	linear regression	994:1010	linear regression	994:1010	Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (α = .05).
30070706	8	22	theme	species	1450:1456	arg1	proportions					1401:1411	lower proportions	1395:1411	lower proportions of the host-compatible green complex species	1395:1456	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	23	dep	sucrose	1252:1258	arg1	4×					1261:1262	4×	1261:1262	4×	1261:1262	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	23	dep	sucrose	1252:1258	arg1	8×					1271:1272	8×	1271:1272	8×	1271:1272	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	5	24	theme	intracellular	800:812	arg1	polysaccharides					814:828	intracellular polysaccharides	800:828	intracellular polysaccharides	800:828	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	8	25	theme	species	1330:1336	arg1	levels					1303:1308	mean total levels	1292:1308	mean total levels of the 40 bacterial species evaluated	1292:1346	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	9	26	theme	biofilms	1632:1639	arg1	composition					1617:1627	the composition	1613:1627	the composition of biofilms formed on titanium surfaces	1613:1667	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	7	27	theme	linear	1110:1115	arg1	relationship					1117:1128	A positive significant linear relationship	1087:1128	A positive significant linear relationship	1087:1128	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	2	28	theme	titanium	408:415	arg1	specimens					417:425	titanium specimens	408:425	titanium specimens	408:425	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	5	29	theme	insoluble	748:756	arg1	IPS					831:833	IPS	831:833	IPS	831:833	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	29	theme	insoluble	748:756	arg1	polysaccharides					780:794	insoluble (I-EPS) extracellular polysaccharides	748:794	insoluble (I-EPS) extracellular polysaccharides	748:794	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	30	theme	biochemical	650:660	arg1	analysis					662:669	biochemical analysis	650:669	biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS)	650:834	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	1	31	theme	dietary	224:230	arg1	sugar					232:236	this dietary sugar	219:236	this dietary sugar	219:236	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	9	32	theme	titanium	1651:1658	arg1	surfaces					1660:1667	titanium surfaces	1651:1667	titanium surfaces	1651:1667	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	5	33	theme	protein	704:710	arg1	concentration					712:724	protein concentration	704:724	protein concentration	704:724	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	9	34	theme	present	1539:1545	arg1	study					1547:1551	the present study	1535:1551	the present study	1535:1551	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	1	35	theme	sugar	232:236	arg1	effect					209:214	the effect	205:214	the effect of this dietary sugar on biofilm formation on titanium surface	205:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	5	36	theme	microbiologic	845:857	arg1	analysis					859:866	microbiologic analysis	845:866	microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species)	845:957	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	37	theme	I-EPS	759:763	arg1	IPS					831:833	IPS	831:833	IPS	831:833	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	37	theme	I-EPS	759:763	arg1	polysaccharides					780:794	insoluble (I-EPS) extracellular polysaccharides	748:794	insoluble (I-EPS) extracellular polysaccharides	748:794	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	38	theme	biofilm	674:680	arg1	weight					686:691	biofilm wet weight	674:691	biofilm wet weight (biomass)	674:701	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	5	38	theme	biofilm	674:680	arg1	biomass					694:700	biomass	694:700	biomass	694:700	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	0	39	theme	sucrose	10:16	arg1	Effect					0:5	Effect	0:5	Effect of sucrose on biofilm	0:27	Effect of sucrose on biofilm formed in situ on titanium material.
30070706	2	40	theme	blind	296:300	arg1	study					322:326	this blind, crossover, in situ study	291:326	this blind, crossover, in situ study	291:326	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	8	41	attach	presented	1275:1283	arg2	biofilms					1230:1237	The biofilms	1226:1237	The biofilms treated with sucrose (4× and/or 8×)	1226:1273	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	41	attach	presented	1275:1283	arg1	comparison					1462:1471	comparison	1462:1471	comparison with the control group (p < 0.05)	1462:1505	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	4	42	theme	no	560:561	arg1	treatment					563:571	no treatment	560:571	no treatment	560:571	As control, no treatment was rendered (0×).
30070706	4	42	theme	no	560:561	arg1	control					551:557	control	551:557	control	551:557	As control, no treatment was rendered (0×).
30070706	1	43	theme	biofilm	241:247	arg1	formation					249:257	biofilm formation	241:257	biofilm formation on titanium surface	241:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	6	44	theme	Dunn	1057:1060	arg1	α =					1069:1071	α = .05	1069:1075	α = .05	1069:1075	Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (α = .05).
30070706	6	44	theme	Dunn	1057:1060	arg1	tests					1062:1066	Dunn tests	1057:1066	Dunn tests (α = .05)	1057:1076	Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (α = .05).
30070706	5	45	theme	weight	686:691	arg1	analysis					662:669	biochemical analysis	650:669	biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS)	650:834	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	3	46	theme	sucrose	491:497	arg1	solution					499:506	20% sucrose solution	487:506	20% sucrose solution	487:506	In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day.
30070706	7	47	theme	sucrose	1146:1152	arg1	exposure					1154:1161	sucrose exposure	1146:1161	sucrose exposure (0×, 4×, and 8×)	1146:1178	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	1	48	theme	study	175:179	arg1	aim					163:165	the aim	159:165	the aim of this study	159:179	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	9	49	theme	study	1547:1551	arg1	findings					1523:1530	The findings	1519:1530	The findings of the present study	1519:1551	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	1	50	from	effect	209:214	arg1	formation					249:257	biofilm formation	241:257	biofilm formation on titanium surface	241:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	3	51	theme	4	526:526	arg1	8 times					531:537	8 times	531:537	8 times per day	531:545	In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day.
30070706	3	51	theme	4	526:526	arg1	frequency					513:521	a frequency	511:521	a frequency of 4	511:526	In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day.
30070706	9	52	theme	daily	1566:1570	arg1	exposure					1580:1587	daily sucrose exposure	1566:1587	daily sucrose exposure	1566:1587	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	5	53	theme	concentration	712:724	arg1	analysis					662:669	biochemical analysis	650:669	biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS)	650:834	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	8	54	theme	control	1482:1488	arg1	p <					1497:1499	p < 0.05	1497:1504	p < 0.05	1497:1504	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	54	theme	control	1482:1488	arg1	group					1490:1494	the control group	1478:1494	the control group (p < 0.05)	1478:1505	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	55	theme	mean	1292:1295	arg1	levels					1303:1308	mean total levels	1292:1308	mean total levels of the 40 bacterial species evaluated	1292:1346	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	1	56	dep	surfaces	149:156	arg1	was					181:183	was	181:183	was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface	181:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	7	57	theme	positive	1089:1096	arg1	relationship					1117:1128	A positive significant linear relationship	1087:1128	A positive significant linear relationship	1087:1128	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	2	58	theme	7 days	364:369	arg1	phases					354:359	3 phases	352:359	3 phases of 7 days each	352:374	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	1	59	theme	titanium	262:269	arg1	surface					271:277	titanium surface	262:277	titanium surface	262:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	3	60	theme	%	489:489	arg1	solution					499:506	20% sucrose solution	487:506	20% sucrose solution	487:506	In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day.
30070706	2	61	contain	containing	397:406	arg1	appliance					387:395	a palatal appliance	377:395	a palatal appliance containing titanium specimens	377:425	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	2	61	contain	containing	397:406	arg2	specimens					417:425	titanium specimens	408:425	titanium specimens	408:425	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	7	62	theme	significant	1098:1108	arg1	relationship					1117:1128	A positive significant linear relationship	1087:1128	A positive significant linear relationship	1087:1128	RESULTS A positive significant linear relationship was found among sucrose exposure (0×, 4×, and 8×) and biomass, S-EPS, I-EPS and IPS (p < 0.05).
30070706	8	63	theme	lower	1395:1399	arg1	proportions					1401:1411	lower proportions	1395:1411	lower proportions of the host-compatible green complex species	1395:1456	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	64	theme	bacterial	1320:1328	arg1	species					1330:1336	the 40 bacterial species	1313:1336	the 40 bacterial species evaluated	1313:1346	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	65	theme	total	1297:1301	arg1	levels					1303:1308	mean total levels	1292:1308	mean total levels of the 40 bacterial species evaluated	1292:1346	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	9	66	theme	sucrose	1572:1578	arg1	exposure					1580:1587	daily sucrose exposure	1566:1587	daily sucrose exposure	1566:1587	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	6	67	theme	Biochemical	960:970	arg1	data					972:975	Biochemical data	960:975	Biochemical data	960:975	Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (α = .05).
30070706	9	68	dep	CONCLUSION	1508:1517	arg1	suggest					1553:1559	suggest	1553:1559	suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces	1553:1667	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	5	69	theme	checkerboard	871:882	arg1	hybridization					892:904	checkerboard DNA-DNA hybridization	871:904	checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species)	871:957	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	2	70	dep	in	314:315	arg1	situ					317:320	situ	317:320	situ	317:320	METHODS In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens.
30070706	0	71	theme	titanium	47:54	arg1	material					56:63	titanium material	47:63	titanium material	47:63	Effect of sucrose on biofilm formed in situ on titanium material.
30070706	5	72	theme	DNA-DNA	884:890	arg1	hybridization					892:904	checkerboard DNA-DNA hybridization	871:904	checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species)	871:957	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	1	73	theme	biofilms	123:130	arg1	composition					108:118	the composition	104:118	the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface	104:277	BACKGROUND Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface.
30070706	9	74	theme	harmful	1595:1601	arg1	effect					1603:1608	a harmful effect	1593:1608	a harmful effect	1593:1608	CONCLUSION The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces.
30070706	8	75	theme	green	1436:1440	arg1	species					1450:1456	the host-compatible green complex species	1416:1456	the host-compatible green complex species	1416:1456	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	76	theme	species	1383:1389	arg1	proportions					1356:1366	higher proportions	1349:1366	higher proportions of red complex species	1349:1389	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	5	77	theme	bacterial	940:948	arg1	species					950:956	40 bacterial species	937:956	40 bacterial species	937:956	At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species).
30070706	6	78	theme	microbiological	1016:1030	arg1	findings					1032:1039	microbiological findings	1016:1039	microbiological findings	1016:1039	Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (α = .05).
30070706	8	79	dep	higher	1285:1290	arg1	levels					1303:1308	mean total levels	1292:1308	mean total levels of the 40 bacterial species evaluated	1292:1346	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	80	theme	host-compatible	1420:1434	arg1	species					1450:1456	the host-compatible green complex species	1416:1456	the host-compatible green complex species	1416:1456	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
30070706	8	81	theme	higher	1349:1354	arg1	proportions					1356:1366	higher proportions	1349:1366	higher proportions of red complex species	1349:1389	The biofilms treated with sucrose (4× and/or 8×) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05).
31054307	1	0	theme	unsaturated	140:150	arg1	lipids					152:157	unsaturated lipids	140:157	unsaturated lipids that are susceptible to oxidation even under frozen storage	140:217	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	3	1	from	composition	459:469	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	1	from	composition	459:469	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	1	from	composition	459:469	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	1	from	composition	459:469	arg1	TBARs					386:390	TBARs	386:390	TBARs	386:390	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	4	2	with	Treatment	513:521	arg1	DP					528:529	DP	528:529	DP	528:529	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	1	3	from	lipids	152:157	arg1	rich					132:135	rich	132:135	rich	132:135	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	2	4	theme	dandelion	268:276	arg1	DP					294:295	DP	294:295	DP	294:295	In this study, Antarctic krill was treated with dandelion polysaccharide (DP) and stored frozen at -8 °C for 12 months.
31054307	2	4	theme	dandelion	268:276	arg1	polysaccharide					278:291	dandelion polysaccharide	268:291	dandelion polysaccharide (DP)	268:296	In this study, Antarctic krill was treated with dandelion polysaccharide (DP) and stored frozen at -8 °C for 12 months.
31054307	1	5	from	rich	132:135	arg1	lipids					152:157	unsaturated lipids	140:157	unsaturated lipids that are susceptible to oxidation even under frozen storage	140:217	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	0	6	theme	Dandelion	0:8	arg1	polysaccharide					10:23	Dandelion polysaccharide	0:23	Dandelion polysaccharide	0:23	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	4	7	theme	acid	634:637	arg1	contents					639:646	the unsaturated fatty acid contents	612:646	the unsaturated fatty acid contents	612:646	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	3	8	from	changes	344:350	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	8	from	changes	344:350	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	8	from	changes	344:350	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	8	from	changes	344:350	arg1	TBARs					386:390	TBARs	386:390	TBARs	386:390	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	9	theme	fatty	448:452	arg1	composition					459:469	fatty acid composition	448:469	fatty acid composition	448:469	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	5	10	theme	krill	776:780	arg1	life					758:761	the shelf life	748:761	the shelf life of Antarctic krill	748:780	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	3	11	theme	peroxide	394:401	arg1	PVs					411:413	PVs	411:413	PVs	411:413	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	11	theme	peroxide	394:401	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	11	theme	peroxide	394:401	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	12	theme	acid	454:457	arg1	composition					459:469	fatty acid composition	448:469	fatty acid composition	448:469	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	5	13	theme	Antarctic	766:774	arg1	krill					776:780	Antarctic krill	766:780	Antarctic krill	766:780	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	5	14	theme	DP	669:670	arg1	way					703:705	a promising way	691:705	a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill	691:780	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	5	14	theme	DP	669:670	arg1	treatment					672:680	DP treatment	669:680	DP treatment	669:680	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	4	15	theme	quality	584:590	arg1	score					592:596	sensory quality score	576:596	sensory quality score	576:596	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	3	16	theme	thiobarbituric	355:368	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	16	theme	thiobarbituric	355:368	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	16	theme	thiobarbituric	355:368	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	16	theme	thiobarbituric	355:368	arg1	TBARs					386:390	TBARs	386:390	TBARs	386:390	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	1	17	theme	Antarctic	92:100	arg1	krill					102:106	Antarctic krill	92:106	Antarctic krill (Euphausia superba)	92:126	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	1	17	theme	Antarctic	92:100	arg1	superba					119:125	superba	119:125	superba	119:125	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	4	18	theme	sensory	576:582	arg1	score					592:596	sensory quality score	576:596	sensory quality score	576:596	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	0	19	theme	lipid	36:40	arg1	oxidation					42:50	lipid oxidation	36:50	lipid oxidation	36:50	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	5	20	theme	lipid	721:725	arg1	oxidation					727:735	lipid oxidation	721:735	lipid oxidation	721:735	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	3	21	theme	sensory	475:481	arg1	score					491:495	sensory quality score	475:495	sensory quality score	475:495	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	4	22	theme	TBAR	550:553	arg1	contents					563:570	the PV, TBAR and FFA contents	542:570	the PV, TBAR and FFA contents	542:570	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	3	23	theme	quality	483:489	arg1	score					491:495	sensory quality score	475:495	sensory quality score	475:495	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	5	24	theme	shelf	752:756	arg1	life					758:761	the shelf life	748:761	the shelf life of Antarctic krill	748:780	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	4	25	theme	FFA	559:561	arg1	contents					563:570	the PV, TBAR and FFA contents	542:570	the PV, TBAR and FFA contents	542:570	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	5	26	theme	promising	693:701	arg1	way					703:705	a promising way	691:705	a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill	691:780	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	5	26	theme	promising	693:701	arg1	treatment					672:680	DP treatment	669:680	DP treatment	669:680	Results showed that DP treatment could be a promising way to suppresses lipid oxidation and extend the shelf life of Antarctic krill.
31054307	1	27	theme	frozen	204:209	arg1	storage					211:217	frozen storage	204:217	frozen storage	204:217	Antarctic krill (Euphausia superba) are rich in unsaturated lipids that are susceptible to oxidation even under frozen storage.
31054307	3	28	theme	acid	370:373	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	28	theme	acid	370:373	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	28	theme	acid	370:373	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	28	theme	acid	370:373	arg1	TBARs					386:390	TBARs	386:390	TBARs	386:390	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	29	theme	acid	428:431	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	29	theme	acid	428:431	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	0	30	theme	Antarctic	55:63	arg1	superba					82:88	Euphausia superba	72:88	Euphausia superba	72:88	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	0	30	theme	Antarctic	55:63	arg1	krill					65:69	Antarctic krill	55:69	Antarctic krill (Euphausia superba)	55:89	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	4	31	theme	unsaturated	616:626	arg1	contents					639:646	the unsaturated fatty acid contents	612:646	the unsaturated fatty acid contents	612:646	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	4	32	theme	fatty	628:632	arg1	contents					639:646	the unsaturated fatty acid contents	612:646	the unsaturated fatty acid contents	612:646	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
31054307	3	33	theme	free	417:420	arg1	acid					428:431	free fatty acid	417:431	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	33	theme	free	417:420	arg1	FFA					434:436	FFA	434:436	FFA	434:436	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	2	34	theme	Antarctic	235:243	arg1	krill					245:249	Antarctic krill	235:249	Antarctic krill	235:249	In this study, Antarctic krill was treated with dandelion polysaccharide (DP) and stored frozen at -8 °C for 12 months.
31054307	3	35	theme	fatty	422:426	arg1	acid					428:431	free fatty acid	417:431	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	35	theme	fatty	422:426	arg1	FFA					434:436	FFA	434:436	FFA	434:436	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	36	from	score	491:495	arg1	reactants					375:383	thiobarbituric acid reactants	355:383	thiobarbituric acid reactants (TBARs)	355:391	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	36	from	score	491:495	arg1	values					403:408	peroxide values	394:408	peroxide values (PVs)	394:414	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	36	from	score	491:495	arg1	content					439:445	free fatty acid (FFA) content	417:445	free fatty acid (FFA) content	417:445	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	3	36	from	score	491:495	arg1	TBARs					386:390	TBARs	386:390	TBARs	386:390	The changes in thiobarbituric acid reactants (TBARs), peroxide values (PVs), free fatty acid (FFA) content, fatty acid composition and sensory quality score were evaluated.
31054307	0	37	theme	Euphausia	72:80	arg1	superba					82:88	Euphausia superba	72:88	Euphausia superba	72:88	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	0	37	theme	Euphausia	72:80	arg1	krill					65:69	Antarctic krill	55:69	Antarctic krill (Euphausia superba)	55:89	Dandelion polysaccharide suppresses lipid oxidation in Antarctic krill (Euphausia superba).
31054307	4	38	theme	PV	546:547	arg1	contents					563:570	the PV, TBAR and FFA contents	542:570	the PV, TBAR and FFA contents	542:570	Treatment with DP maintained the PV, TBAR and FFA contents and sensory quality score and increased the unsaturated fatty acid contents.
30412830	6	0	link	-linked	1084:1090	arg1	chains					1125:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains.
30412830	5	1	dep	structures	901:910	arg1	β-d-glucan					930:939	a (1 → 6)-linked β-d-glucan	913:939	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	4	2	attach	present	798:804	arg2	β-d-glucans					774:784	β-d-glucans	774:784	β-d-glucans	774:784	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	4	2	attach	present	798:804	arg1	latter					813:818	latter	813:818	latter	813:818	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	1	3	theme	dense	202:206	arg1	mushroom					208:215	dense mushroom	202:215	dense mushroom	202:215	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	2	4	theme	enzymatic	307:315	arg1	extraction					295:304	sequential extraction	284:304	sequential extraction	284:304	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	2	4	theme	enzymatic	307:315	arg1	treatment					317:325	enzymatic treatment	307:325	enzymatic treatment	307:325	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	5	5	link	-linked	996:1002	arg1	β-d-glucan					1004:1013	a (1 → 3)-linked β-d-glucan	987:1013	O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	974:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	5	6	link	-linked	922:928	arg1	β-d-glucan					930:939	a (1 → 6)-linked β-d-glucan	913:939	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	3	7	theme	size	649:652	arg1	chromatography					664:677	size exclusion chromatography	649:677	size exclusion chromatography	649:677	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	3	8	theme	-α-d-galactan	495:507	arg1	backbone					509:516	an (1 → 6)-α-d-galactan backbone	485:516	an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2	485:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	3	9	attach	attached	548:555	arg2	residues					539:546	single α-l-Fucp residues	523:546	single α-l-Fucp residues attached at O-2	523:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	3	9	attach	attached	548:555	arg3	O-2					560:562	O-2	560:562	O-2	560:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	0	10	theme	branched	87:94	arg1	-β-d-glucan					103:113	a branched (1 → 6)-β-d-glucan	85:113	a branched (1 → 6)-β-d-glucan	85:113	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	6	11	theme	-α-d-mannan	1113:1123	arg1	chains					1125:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains.
30412830	4	12	theme	hot	715:717	arg1	extract					726:732	the hot alkali extract	711:732	the hot alkali extract	711:732	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	3	13	with	treatment	619:627	arg1	chromatography					664:677	size exclusion chromatography	649:677	size exclusion chromatography	649:677	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	3	13	with	treatment	619:627	arg1	protease					636:643	protease	636:643	protease	636:643	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	3	14	dep	protease	636:643	arg1	a					634:634	a	634:634	a	634:634	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	4	15	located	present	798:804	arg2	β-d-glucans					774:784	β-d-glucans	774:784	β-d-glucans	774:784	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	4	15	located	present	798:804	arg1	latter					813:818	latter	813:818	latter	813:818	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	5	16	theme	single	946:951	arg1	residues					962:969	single β-d-Glcp residues	946:969	single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	946:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	2	17	theme	glycosidic	384:393	arg1	linkages					395:402	glycosidic linkages	384:402	glycosidic linkages by methylation and GC-MS	384:427	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	3	18	theme	1 → 6	489:493	arg1	backbone					509:516	an (1 → 6)-α-d-galactan backbone	485:516	an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2	485:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	5	19	theme	β-d-glucan	881:890	arg1	structures					901:910	two different β-d-glucan backbone structures	867:910	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	2	20	theme	monosaccharide	356:369	arg1	composition					371:381	monosaccharide composition	356:381	monosaccharide composition	356:381	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	5	21	from	β-d-glucan	1004:1013	arg1	residues					962:969	single β-d-Glcp residues	946:969	single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	946:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	4	22	from	present	798:804	arg1	latter					813:818	latter	813:818	latter	813:818	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	1	23	from	material	241:248	arg1	rich					223:226	rich	223:226	rich	223:226	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	5	24	theme	structures	901:910	arg1	presence					855:862	the presence	851:862	the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	851:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	1	25	theme	Albatrellus	150:160	arg1	ovinus					162:167	Albatrellus ovinus	150:167	Albatrellus ovinus	150:167	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	1	25	theme	Albatrellus	150:160	arg1	large					195:199	large	195:199	large	195:199	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	1	25	theme	Albatrellus	150:160	arg1	polypore					180:187	the sheep polypore	170:187	the sheep polypore	170:187	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	0	26	theme	edible	4:9	arg1	mushroom					11:18	The edible mushroom	0:18	The edible mushroom Albatrellus ovinus	0:37	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	5	27	theme	β-d-Glcp	953:960	arg1	residues					962:969	single β-d-Glcp residues	946:969	single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	946:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	3	28	theme	α-l-Fucp	530:537	arg1	residues					539:546	single α-l-Fucp residues	523:546	single α-l-Fucp residues attached at O-2	523:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	6	29	dep	-linked	1084:1090	arg1	β-d-xylan					1092:1100	β-d-xylan	1092:1100	β-d-xylan	1092:1100	In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains.
30412830	5	30	theme	Structural	821:830	arg1	analysis					832:839	Structural analysis	821:839	Structural analysis	821:839	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	3	31	theme	single	523:528	arg1	residues					539:546	single α-l-Fucp residues	523:546	single α-l-Fucp residues attached at O-2	523:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	4	32	theme	water	693:697	arg1	extract					699:705	the hot water extract	685:705	the hot water extract	685:705	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	4	33	theme	alkali	719:724	arg1	extract					726:732	the hot alkali extract	711:732	the hot alkali extract	711:732	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	0	34	theme	Albatrellus	20:30	arg1	mushroom					11:18	The edible mushroom	0:18	The edible mushroom Albatrellus ovinus	0:37	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	1	35	theme	cell	231:234	arg1	material					241:248	cell wall material	231:248	cell wall material	231:248	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	0	36	contain	contains	39:46	arg1	mushroom					11:18	The edible mushroom	0:18	The edible mushroom Albatrellus ovinus	0:37	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	0	36	contain	contains	39:46	arg2	-β-d-glucan					103:113	a branched (1 → 6)-β-d-glucan	85:113	a branched (1 → 6)-β-d-glucan	85:113	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	0	36	contain	contains	39:46	arg2	-β-d-glucan					137:147	a branched (1 → 3)-β-d-glucan	119:147	a branched (1 → 3)-β-d-glucan	119:147	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	1	37	theme	wall	236:239	arg1	material					241:248	cell wall material	231:248	cell wall material	231:248	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	1	38	from	rich	223:226	arg1	material					241:248	cell wall material	231:248	cell wall material	231:248	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	5	39	theme	-linked	996:1002	arg1	β-d-glucan					1004:1013	a (1 → 3)-linked β-d-glucan	987:1013	O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	974:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	0	40	dep	-β-d-glucan	103:113	arg1	1 → 6					97:101	1 → 6	97:101	1 → 6	97:101	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	1	41	theme	sheep	174:178	arg1	ovinus					162:167	Albatrellus ovinus	150:167	Albatrellus ovinus	150:167	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	1	41	theme	sheep	174:178	arg1	polypore					180:187	the sheep polypore	170:187	the sheep polypore	170:187	Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material.
30412830	2	42	theme	NMR	440:442	arg1	spectroscopy					444:455	NMR spectroscopy	440:455	NMR spectroscopy	440:455	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	0	43	theme	branched	121:128	arg1	-β-d-glucan					137:147	a branched (1 → 3)-β-d-glucan	119:147	a branched (1 → 3)-β-d-glucan	119:147	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	0	44	dep	α-l-fuco-α-d-galactan	50:70	arg1	α-d-glucan					73:82	α-d-glucan	73:82	α-d-glucan	73:82	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	5	45	from	O-3	974:976	arg1	residues					962:969	single β-d-Glcp residues	946:969	single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	946:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	5	46	with	β-d-glucan	930:939	arg1	residues					962:969	single β-d-Glcp residues	946:969	single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	946:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	3	47	theme	water	590:594	arg1	extract					596:602	the hot water extract	582:602	the hot water extract obtained after treatment with a protease and size exclusion chromatography	582:677	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	0	48	theme	α-l-fuco-α-d-galactan	50:70	arg1	-β-d-glucan					103:113	a branched (1 → 6)-β-d-glucan	85:113	a branched (1 → 6)-β-d-glucan	85:113	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	4	49	contain	contained	734:742	arg1	extract					726:732	the hot alkali extract	711:732	the hot alkali extract	711:732	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	4	49	contain	contained	734:742	arg1	extract					699:705	the hot water extract	685:705	the hot water extract	685:705	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	4	49	contain	contained	734:742	arg2	-α-d-glucan					754:764	an (1 → 4)-α-d-glucan	744:764	an (1 → 4)-α-d-glucan	744:764	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	0	50	dep	-β-d-glucan	137:147	arg1	1 → 3					131:135	1 → 3	131:135	1 → 3	131:135	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	3	51	with	backbone	509:516	arg1	residues					539:546	single α-l-Fucp residues	523:546	single α-l-Fucp residues attached at O-2	523:562	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	5	52	theme	different	871:879	arg1	structures					901:910	two different β-d-glucan backbone structures	867:910	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	0	53	dep	Albatrellus	20:30	arg1	ovinus					32:37	ovinus	32:37	ovinus	32:37	The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan.
30412830	2	54	theme	sequential	284:293	arg1	extraction					295:304	sequential extraction	284:304	sequential extraction	284:304	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	2	54	theme	sequential	284:293	arg1	treatment					317:325	enzymatic treatment	307:325	enzymatic treatment	307:325	Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy.
30412830	6	55	theme	short	1071:1075	arg1	chains					1125:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains.
30412830	6	56	theme	-linked	1084:1090	arg1	chains					1125:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains	1071:1130	In addition there were identified short (1 → 2)-linked β-d-xylan and (1 → 3)-α-d-mannan chains.
30412830	3	57	theme	hot	586:588	arg1	extract					596:602	the hot water extract	582:602	the hot water extract obtained after treatment with a protease and size exclusion chromatography	582:677	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	5	58	theme	backbone	892:899	arg1	structures					901:910	two different β-d-glucan backbone structures	867:910	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	4	59	theme	hot	689:691	arg1	extract					699:705	the hot water extract	685:705	the hot water extract	685:705	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	5	60	theme	-linked	922:928	arg1	β-d-glucan					930:939	a (1 → 6)-linked β-d-glucan	913:939	two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6	867:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	4	61	from	latter	813:818	arg1	present					798:804	present	798:804	present	798:804	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	5	62	with	β-d-glucan	1004:1013	arg1	branches					1020:1027	branches	1020:1027	branches in O-6	1020:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
30412830	3	63	theme	exclusion	654:662	arg1	chromatography					664:677	size exclusion chromatography	649:677	size exclusion chromatography	649:677	A fucogalactan composed of an (1 → 6)-α-d-galactan backbone with single α-l-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography.
30412830	4	64	theme	1 → 4	748:752	arg1	-α-d-glucan					754:764	an (1 → 4)-α-d-glucan	744:764	an (1 → 4)-α-d-glucan	744:764	Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d-glucan whereas β-d-glucans were mainly present in the latter.
30412830	5	65	from	branches	1020:1027	arg1	O-6					1032:1034	O-6	1032:1034	O-6	1032:1034	Structural analysis suggested the presence of two different β-d-glucan backbone structures; a (1 → 6)-linked β-d-glucan with single β-d-Glcp residues at O-3 and also a (1 → 3)-linked β-d-glucan with branches in O-6.
29887307	9	0	theme	matrix	1781:1786	arg1	components					1788:1797	two matrix components	1777:1797	two matrix components	1777:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	7	1	theme	incomplete	998:1007	arg1	specialization					1020:1033	This incomplete phenotypic specialization	993:1033	This incomplete phenotypic specialization	993:1033	This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPSs and TasA.
29887307	1	2	theme	group	134:138	arg1	motility					140:147	group motility	134:147	group motility	134:147	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	9	3	theme	components	1788:1797	arg1	production					1763:1772	the simultaneous production	1746:1772	the simultaneous production of two matrix components	1746:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	9	4	theme	strain	1526:1531	arg1	ratio					1533:1537	strain ratio	1526:1537	strain ratio	1526:1537	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	8	5	theme	TasA	1358:1361	arg1	producers					1363:1371	30% TasA producers	1354:1371	30% TasA producers	1354:1371	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	8	6	theme	frequency	1244:1252	arg1	dependence					1254:1263	a negative frequency dependence	1233:1263	a negative frequency dependence	1233:1263	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	5	7	theme	major	742:746	arg1	components					748:757	two major components	738:757	two major components	738:757	In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA.
29887307	9	8	theme	labor	1672:1676	arg1	division					1660:1667	genetic division	1652:1667	genetic division of labor	1652:1676	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	8	9	theme	30	1354:1355	arg1	%					1356:1356	%	1356:1356	%	1356:1356	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	9	10	theme	matrix	1598:1603	arg1	compounds					1605:1613	matrix compounds	1598:1613	matrix compounds	1598:1613	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	4	11	theme	labor	606:610	arg1	division					594:601	a division	592:601	a division of labor	592:610	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
29887307	6	12	theme	EPS	927:929	arg1	producers					931:939	EPS producers	927:939	EPS producers	927:939	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	2	13	theme	clonal	262:267	arg1	subpopulations					269:282	phenotypically specialized clonal subpopulations	235:282	phenotypically specialized clonal subpopulations	235:282	Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks.
29887307	6	14	theme	clonal	813:818	arg1	B. subtilis					830:840	B. subtilis	830:840	B. subtilis	830:840	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	6	14	theme	clonal	813:818	arg1	groups					820:825	clonal groups	813:825	clonal groups of B. subtilis	813:840	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	3	15	theme	matrix	517:522	arg1	production					524:533	biofilm matrix production	509:533	biofilm matrix production	509:533	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	8	16	theme	strain	1304:1309	arg1	frequency					1311:1319	strain frequency	1304:1319	strain frequency	1304:1319	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	8	17	theme	relative	1187:1194	arg1	fitness					1196:1202	The relative fitness	1183:1202	The relative fitness of the two mutants	1183:1221	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	1	18	theme	simple	65:70	arg1	bacteria					75:82	simple as bacteria	65:82	simple as bacteria	65:82	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	8	19	theme	%	1356:1356	arg1	producers					1363:1371	30% TasA producers	1354:1371	30% TasA producers	1354:1371	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	1	20	theme	as	72:73	arg1	bacteria					75:82	simple as bacteria	65:82	simple as bacteria	65:82	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	1	21	theme	fruiting	153:160	arg1	formation					167:175	fruiting body formation	153:175	fruiting body formation	153:175	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	6	22	theme	matrix	905:910	arg1	non-producers					912:924	matrix non-producers	905:924	matrix non-producers	905:924	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	9	23	dep	benefits	1584:1591	arg1	favored					1685:1691	favored	1685:1691	can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components	1678:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	9	23	dep	benefits	1584:1591	arg1	generate					1615:1622	generate	1615:1622	generate for the collective	1615:1641	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	9	24	theme	relative	1575:1582	arg1	benefits					1584:1591	the relative benefits	1571:1591	the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components	1571:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	1	25	theme	body	162:165	arg1	formation					167:175	fruiting body formation	153:175	fruiting body formation	153:175	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	4	26	theme	genetic	569:575	arg1	strategies					577:586	both phenotypic and genetic strategies	549:586	both phenotypic and genetic strategies for a division of labor	549:610	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
29887307	2	27	theme	distinct	299:306	arg1	lineages					308:315	genetically distinct lineages	287:315	genetically distinct lineages	287:315	Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks.
29887307	9	28	theme	individual-based	1471:1486	arg1	modeling					1488:1495	individual-based modeling	1471:1495	individual-based modeling	1471:1495	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	9	29	from	asymmetries	1511:1521	arg1	ratio					1533:1537	strain ratio	1526:1537	strain ratio	1526:1537	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	8	30	dep	dependence	1254:1263	arg1	both					1265:1268	both	1265:1268	both	1265:1268	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	0	31	theme	Labor	12:16	arg1	Division					0:7	Division	0:7	Division of Labor during Biofilm Matrix Production.	0:50	Division of Labor during Biofilm Matrix Production.
29887307	2	32	theme	specialized	250:260	arg1	subpopulations					269:282	phenotypically specialized clonal subpopulations	235:282	phenotypically specialized clonal subpopulations	235:282	Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks.
29887307	8	33	theme	stable	1332:1337	arg1	equilibrium					1339:1349	a stable equilibrium	1330:1349	a stable equilibrium	1330:1349	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	0	34	theme	Matrix	33:38	arg1	Production					40:49	Biofilm Matrix Production	25:49	Biofilm Matrix Production	25:49	Division of Labor during Biofilm Matrix Production.
29887307	3	35	theme	biofilm	509:515	arg1	production					524:533	biofilm matrix production	509:533	biofilm matrix production	509:533	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	4	36	theme	soil	660:663	arg1	bacterium					665:673	the soil bacterium	656:673	the soil bacterium Bacillus subtilis	656:691	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
29887307	3	37	theme	computational	412:424	arg1	approaches					426:435	experimental and computational approaches	395:435	experimental and computational approaches	395:435	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	0	38	theme	Biofilm	25:31	arg1	Production					40:49	Biofilm Matrix Production	25:49	Biofilm Matrix Production	25:49	Division of Labor during Biofilm Matrix Production.
29887307	8	39	theme	plant	1286:1290	arg1	roots					1292:1296	plant roots	1286:1296	plant roots	1286:1296	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	8	40	theme	negative	1235:1242	arg1	dependence					1254:1263	a negative frequency dependence	1233:1263	a negative frequency dependence	1233:1263	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	5	41	theme	In this	694:700	arg1	species					702:708	In this species	694:708	In this species	694:708	In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA.
29887307	5	41	theme	In this	694:700	arg1	matrix					719:724	biofilm matrix	711:724	biofilm matrix	711:724	In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA.
29887307	9	42	theme	simultaneous	1750:1761	arg1	production					1763:1772	the simultaneous production	1746:1772	the simultaneous production of two matrix components	1746:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	1	43	theme	complex	98:104	arg1	actions					117:123	complex collective actions	98:123	complex collective actions	98:123	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	1	43	theme	complex	98:104	arg1	formation					167:175	fruiting body formation	153:175	fruiting body formation	153:175	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	1	43	theme	complex	98:104	arg1	motility					140:147	group motility	134:147	group motility	134:147	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	3	44	theme	experimental	395:406	arg1	approaches					426:435	experimental and computational approaches	395:435	experimental and computational approaches	395:435	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	4	45	theme	phenotypic	554:563	arg1	strategies					577:586	both phenotypic and genetic strategies	549:586	both phenotypic and genetic strategies for a division of labor	549:610	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
29887307	7	46	theme	labor	1077:1081	arg1	division					1065:1072	a genetic division	1055:1072	a genetic division	1055:1072	This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPSs and TasA.
29887307	8	47	theme	population	1403:1412	arg1	composition					1414:1424	the population composition	1399:1424	the population composition where group productivity is maximized	1399:1462	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	8	48	from	dependence	1254:1263	arg1	roots					1292:1296	plant roots	1286:1296	plant roots	1286:1296	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	1	49	theme	collective	106:115	arg1	actions					117:123	complex collective actions	98:123	complex collective actions	98:123	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	1	49	theme	collective	106:115	arg1	formation					167:175	fruiting body formation	153:175	fruiting body formation	153:175	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	1	49	theme	collective	106:115	arg1	motility					140:147	group motility	134:147	group motility	134:147	Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation.
29887307	7	50	theme	genetic	1057:1063	arg1	division					1065:1072	a genetic division	1055:1072	a genetic division	1055:1072	This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPSs and TasA.
29887307	8	51	theme	group	1432:1436	arg1	productivity					1438:1449	group productivity	1432:1449	group productivity	1432:1449	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	5	52	theme	biofilm	711:717	arg1	species					702:708	In this species	694:708	In this species	694:708	In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA.
29887307	5	52	theme	biofilm	711:717	arg1	matrix					719:724	biofilm matrix	711:724	biofilm matrix	711:724	In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA.
29887307	7	53	theme	phenotypic	1009:1018	arg1	specialization					1020:1033	This incomplete phenotypic specialization	993:1033	This incomplete phenotypic specialization	993:1033	This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPSs and TasA.
29887307	3	54	theme	labor	496:500	arg1	division					484:491	division	484:491	division of labor during biofilm matrix production	484:533	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	8	55	theme	mutants	1215:1221	arg1	fitness					1196:1202	The relative fitness	1183:1202	The relative fitness of the two mutants	1183:1221	The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized.
29887307	6	56	theme	B. subtilis	830:840	arg1	B. subtilis					830:840	B. subtilis	830:840	B. subtilis	830:840	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	6	56	theme	B. subtilis	830:840	arg1	groups					820:825	clonal groups	813:825	clonal groups of B. subtilis	813:840	We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA.
29887307	2	57	theme	labor	222:226	arg1	division					210:217	a division	208:217	a division	208:217	Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks.
29887307	9	58	from	differences	1556:1566	arg1	benefits					1584:1591	the relative benefits	1571:1591	the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components	1571:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	3	59	theme	potential	452:460	arg1	benefits					462:469	potential benefits	452:469	potential benefits arising from division of labor during biofilm matrix production	452:533	Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production.
29887307	4	60	theme	biofilm	635:641	arg1	formation					643:651	collective biofilm formation	624:651	collective biofilm formation	624:651	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
29887307	9	61	theme	genetic	1652:1658	arg1	division					1660:1667	genetic division	1652:1667	genetic division of labor	1652:1676	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	2	62	theme	complementary	357:369	arg1	tasks					371:375	complementary tasks	357:375	complementary tasks	357:375	Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks.
29887307	9	63	theme	metabolic	1708:1716	arg1	constraints					1718:1728	metabolic constraints	1708:1728	metabolic constraints associated with the simultaneous production of two matrix components	1708:1797	Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
29887307	4	64	theme	collective	624:633	arg1	formation					643:651	collective biofilm formation	624:651	collective biofilm formation	624:651	We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis.
31539669	4	0	theme	β-d-GalpNAc-	869:880	arg1	linkages					896:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages	869:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units	869:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	4	1	theme	K116	781:784	arg1	CPSs					786:789	the K128 and K116 CPSs	768:789	CPSs	786:789	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	6	2	theme	Wzy	1159:1161	arg1	polymerase					1163:1172	the Wzy polymerase	1155:1172	the Wzy polymerase	1155:1172	Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase.
31539669	5	3	theme	chromosomal	1003:1013	arg1	locus					1017:1021	the chromosomal K locus	999:1021	the chromosomal K locus	999:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	6	4	theme	glycosyltransferase	1113:1131	arg1	gene					1133:1136	one glycosyltransferase gene	1109:1136	one glycosyltransferase gene	1109:1136	Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase.
31539669	1	5	dep	1D	298:299	arg1	1H					308:309	1H	308:309	1H	308:309	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	1	5	dep	1D	298:299	arg1	spectroscopy					323:334	13C NMR spectroscopy	315:334	13C NMR spectroscopy	315:334	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	2	6	theme	A.	511:512	arg1	CPS					529:531	the A. baumannii K116 CPS	507:531	the A. baumannii K116 CPS	507:531	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	5	7	theme	K	1015:1015	arg1	locus					1017:1021	the chromosomal K locus	999:1021	the chromosomal K locus	999:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	5	8	from	locus	1017:1021	arg1	cluster					988:994	the CPS biosynthesis gene cluster	962:994	the CPS biosynthesis gene cluster at the chromosomal K locus	962:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	4	9	theme	repeating	749:757	arg1	units					759:763	the repeating units	745:763	the repeating units in the K128 and K116 CPSs	745:789	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	7	10	theme	different	1287:1295	arg1	linkage					1324:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage	1283:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128	1283:1338	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	1	11	theme	Smith	269:273	arg1	degradation					275:285	Smith degradation	269:285	Smith degradation	269:285	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	4	12	theme	β-d-GalpNAc-	837:848	arg1	-d-Galp					856:862	β-d-GalpNAc-(1 → 4)-d-Galp	837:862	β-d-GalpNAc-(1 → 4)-d-Galp	837:862	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	5	13	theme	CPS	966:968	arg1	cluster					988:994	the CPS biosynthesis gene cluster	962:994	the CPS biosynthesis gene cluster at the chromosomal K locus	962:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	3	14	from	linkage	707:713	arg1	K116					718:721	K116	718:721	K116	718:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	7	15	theme	β-d-GalpNAc-	1297:1308	arg1	linkage					1324:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage	1283:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128	1283:1338	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	7	16	from	gtr76	1230:1234	arg1	KL116					1239:1243	KL116	1239:1243	KL116	1239:1243	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	5	17	theme	biosynthesis	970:981	arg1	cluster					988:994	the CPS biosynthesis gene cluster	962:994	the CPS biosynthesis gene cluster at the chromosomal K locus	962:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	2	18	theme	pentasaccharide	378:392	arg1	units					404:408	branched pentasaccharide repeating units	369:408	branched pentasaccharide repeating units containing only neutral sugars	369:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	4	19	theme	1 → 4	850:854	arg1	-d-Galp					856:862	β-d-GalpNAc-(1 → 4)-d-Galp	837:862	β-d-GalpNAc-(1 → 4)-d-Galp	837:862	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	3	20	theme	1 → 6	662:666	arg1	linkage					669:675	a β-(1 → 6) linkage	657:675	a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116	657:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	4	21	theme	1 → 3	882:886	arg1	linkages					896:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages	869:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units	869:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	7	22	theme	1 → 6	1310:1314	arg1	linkage					1324:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage	1283:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128	1283:1338	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	2	23	theme	branched	369:376	arg1	units					404:408	branched pentasaccharide repeating units	369:408	branched pentasaccharide repeating units containing only neutral sugars	369:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	4	24	from	units	759:763	arg1	CPSs					786:789	the K128 and K116 CPSs	768:789	CPSs	786:789	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	4	25	theme	K128	772:775	arg1	CPSs					786:789	the K128 and K116 CPSs	768:789	CPSs	786:789	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	7	26	theme	-d-Galp	1316:1322	arg1	linkage					1324:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage	1283:1330	the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128	1283:1338	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	2	27	dep	A.	511:512	arg1	baumannii					514:522	baumannii	514:522	baumannii	514:522	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	6	28	with	Consistent	1045:1054	arg1	structures					1069:1078	the CPS structures	1061:1078	the CPS structures	1061:1078	Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase.
31539669	0	29	theme	K128	17:20	arg1	polysaccharide					31:44	the K128 capsular polysaccharide	13:44	the K128 capsular polysaccharide	13:44	Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan.
31539669	3	30	theme	β-	697:698	arg1	linkage					707:713	a β-(1 → 4) linkage	695:713	a β-(1 → 4) linkage in K116	695:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	2	31	theme	neutral	426:432	arg1	sugars					434:439	only neutral sugars	421:439	only neutral sugars	421:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	4	32	theme	K128	816:819	arg1	units					821:825	K128 units	816:825	K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units	816:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	4	33	with	different	800:808	arg1	units					821:825	K128 units	816:825	K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units	816:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	0	34	theme	polysaccharide	31:44	arg1	Structure					0:8	Structure	0:8	Structure of the K128 capsular polysaccharide	0:44	Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan.
31539669	8	35	theme	β-d-GalpNAc-	1400:1411	arg1	linkage					1427:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage	1396:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units	1396:1456	Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.
31539669	2	36	theme	K116	524:527	arg1	CPS					529:531	the A. baumannii K116 CPS	507:531	the A. baumannii K116 CPS	507:531	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	1	37	dep	Acinetobacter	175:187	arg1	baumannii					189:197	Acinetobacter baumannii	175:197	Acinetobacter baumannii isolate KZ-1093 from Kazakhstan	175:229	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	1	37	dep	Acinetobacter	175:187	arg1	isolate					199:205	isolate	199:205	isolate KZ-1093 from Kazakhstan	199:229	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	6	38	theme	CPS	1065:1067	arg1	structures					1069:1078	the CPS structures	1061:1078	the CPS structures	1061:1078	Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase.
31539669	7	39	from	linkage	1324:1330	arg1	K128					1335:1338	K128	1335:1338	K128	1335:1338	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	0	40	theme	capsular	22:29	arg1	polysaccharide					31:44	the K128 capsular polysaccharide	13:44	the K128 capsular polysaccharide	13:44	Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan.
31539669	3	41	theme	disaccharide	608:619	arg1	chain					626:630	the disaccharide side chain	604:630	the disaccharide side chain	604:630	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	3	42	from	linkage	669:675	arg1	K128					680:683	K128	680:683	K128 replacing a β-(1 → 4) linkage in K116	680:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	2	43	theme	repeating	394:402	arg1	units					404:408	branched pentasaccharide repeating units	369:408	branched pentasaccharide repeating units containing only neutral sugars	369:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	4	44	theme	-d-Galp	888:894	arg1	linkages					896:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages	869:903	β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units	869:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	8	45	theme	1 → 4	1413:1417	arg1	linkage					1427:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage	1396:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units	1396:1456	Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.
31539669	3	46	theme	side	621:624	arg1	chain					626:630	the disaccharide side chain	604:630	the disaccharide side chain	604:630	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	3	47	theme	oligosaccharide	552:566	arg1	units					568:572	oligosaccharide units	552:572	oligosaccharide units	552:572	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	5	48	theme	gene	983:986	arg1	cluster					988:994	the CPS biosynthesis gene cluster	962:994	the CPS biosynthesis gene cluster at the chromosomal K locus	962:1021	The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128.
31539669	1	49	theme	NMR	319:321	arg1	spectroscopy					323:334	13C NMR spectroscopy	315:334	13C NMR spectroscopy	315:334	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	8	50	theme	-d-Galp	1419:1425	arg1	linkage					1427:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage	1396:1433	the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units	1396:1456	Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.
31539669	1	51	theme	13C	315:317	arg1	spectroscopy					323:334	13C NMR spectroscopy	315:334	13C NMR spectroscopy	315:334	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	1	52	theme	K128	128:131	arg1	CPS					158:160	CPS	158:160	CPS	158:160	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	1	52	theme	K128	128:131	arg1	polysaccharide					142:155	the K128 capsular polysaccharide	124:155	the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan	124:229	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	3	53	theme	1 → 4	700:704	arg1	linkage					707:713	a β-(1 → 4) linkage	695:713	a β-(1 → 4) linkage in K116	695:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	2	54	contain	containing	410:419	arg1	units					404:408	branched pentasaccharide repeating units	369:408	branched pentasaccharide repeating units containing only neutral sugars	369:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	2	54	contain	containing	410:419	arg2	sugars					434:439	only neutral sugars	421:439	only neutral sugars	421:439	The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS.
31539669	1	55	theme	capsular	133:140	arg1	CPS					158:160	CPS	158:160	CPS	158:160	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	1	55	theme	capsular	133:140	arg1	polysaccharide					142:155	the K128 capsular polysaccharide	124:155	the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan	124:229	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	8	56	theme	WzyK128	1356:1362	arg1	polymerase					1364:1373	the WzyK128 polymerase	1352:1373	the WzyK128 polymerase	1352:1373	Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.
31539669	3	57	theme	main	640:643	arg1	chain					645:649	the main chain	636:649	the main chain	636:649	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	7	58	theme	glycosyltransferase	1196:1214	arg1	gene					1216:1219	the gtr200 glycosyltransferase gene	1185:1219	the gtr200 glycosyltransferase gene	1185:1219	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	1	59	theme	polysaccharide	142:155	arg1	structure					111:119	The structure	107:119	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan	107:229	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31539669	7	60	theme	gtr200	1189:1194	arg1	gene					1216:1219	the gtr200 glycosyltransferase gene	1185:1219	the gtr200 glycosyltransferase gene	1185:1219	In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128.
31539669	0	61	theme	Acinetobacter	58:70	arg1	baumannii					72:80	Acinetobacter baumannii KZ-1093	58:88	Acinetobacter baumannii KZ-1093	58:88	Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan.
31539669	3	62	theme	β-	659:660	arg1	linkage					669:675	a β-(1 → 6) linkage	657:675	a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116	657:721	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	4	63	theme	K116	913:916	arg1	units					918:922	K116 units	913:922	K116 units	913:922	The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units.
31539669	3	64	dep	K128	538:541	arg1	units					568:572	oligosaccharide units	552:572	oligosaccharide units	552:572	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	3	64	dep	K128	538:541	arg1	The					534:536	The	534:536	The	534:536	The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116.
31539669	8	65	theme	K128	1447:1450	arg1	units					1452:1456	the K128 units	1443:1456	the K128 units	1443:1456	Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.
31539669	1	66	theme	sugar	250:254	arg1	analysis					256:263	sugar analysis	250:263	sugar analysis	250:263	The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy.
31152228	8	0	theme	mass	1223:1226	arg1	compositions					1228:1239	deuterium-containing mass compositions	1202:1239	deuterium-containing mass compositions	1202:1239	The N- and O-sulfates are differentiated by deuterium-containing mass compositions.
31152228	6	1	theme	synthetic	997:1005	arg1	Arixtra®					1021:1028	Arixtra®	1021:1028	Arixtra®	1021:1028	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	6	1	theme	synthetic	997:1005	arg1	pentamer					1011:1018	a synthetic GAG pentamer	995:1018	a synthetic GAG pentamer	995:1018	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	9	2	theme	uronic	1246:1251	arg1	methylesters					1253:1264	The uronic methylesters	1242:1264	The uronic methylesters	1242:1264	The uronic methylesters do not significantly alter the fragmentation patterns.
31152228	3	3	theme	MSn	554:556	arg1	disassembly					558:568	MSn disassembly	554:568	MSn disassembly for a detailed structural characterization	554:611	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	10	4	theme	products	1349:1356	arg1	library					1332:1338	A fragment library	1321:1338	A fragment library of these products	1321:1356	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	10	4	theme	products	1349:1356	arg1	adjunct					1383:1389	an adjunct	1380:1389	an adjunct to our larger fragment library	1380:1420	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	8	5	theme	deuterium-containing	1202:1221	arg1	compositions					1228:1239	deuterium-containing mass compositions	1202:1239	deuterium-containing mass compositions	1202:1239	The N- and O-sulfates are differentiated by deuterium-containing mass compositions.
31152228	3	6	theme	sulfate	404:410	arg1	lability					412:419	sulfate lability	404:419	sulfate lability	404:419	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	6	7	theme	chemical	939:946	arg1	modifications					948:960	these chemical modifications	933:960	these chemical modifications	933:960	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	6	7	theme	chemical	939:946	arg1	steps					915:919	documenting steps	903:919	documenting steps	903:919	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	4	8	theme	ideal	767:771	arg1	product					759:765	a stable product	750:765	a stable product ideal for sequencing by MSn	750:793	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	2	9	from	difficulty	252:261	arg1	characterization					295:310	the comprehensive structural characterization	266:310	the comprehensive structural characterization of GAGs	266:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	4	10	theme	sulfate	648:654	arg1	release					656:662	sulfate release	648:662	sulfate release	648:662	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	3	11	theme	collisional	516:526	arg1	dissociation					536:547	collisional induced dissociation	516:547	collisional induced dissociation	516:547	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	3	12	theme	detailed	576:583	arg1	characterization					596:611	a detailed structural characterization	574:611	a detailed structural characterization	574:611	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	5	13	theme	detailed	836:843	arg1	read-out					845:852	a detailed read-out	834:852	a detailed read-out of sequence inclusive of all sulfation sites	834:897	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	5	14	theme	samples	817:823	arg1	Disassembly					796:806	Disassembly	796:806	Disassembly of these samples	796:823	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	2	15	theme	GAGs	315:318	arg1	characterization					295:310	the comprehensive structural characterization	266:310	the comprehensive structural characterization of GAGs	266:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	0	16	theme	MSn	10:12	arg1	resolution					22:31	MSn spatial resolution	10:31	MSn spatial resolution	10:31	Advancing MSn spatial resolution and documentation for glycosaminoglycans by sulfate-isotope exchange.
31152228	7	17	theme	sulfation	1137:1145	arg1	positions					1147:1155	individual sulfation positions	1126:1155	individual sulfation positions	1126:1155	Upon disassembly, glycosidic and cross-ring cleavages define the monomer composition including individual sulfation positions.
31152228	2	18	theme	position	363:370	arg1	isomers					372:378	specific sulfation position isomers	344:378	specific sulfation position isomers	344:378	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	6	19	theme	pentamer	1011:1018	arg1	series					967:972	a series	965:972	a series of disaccharides and a synthetic GAG pentamer, Arixtra®	965:1028	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	9	20	theme	fragmentation	1297:1309	arg1	patterns					1311:1318	the fragmentation patterns	1293:1318	the fragmentation patterns	1293:1318	The uronic methylesters do not significantly alter the fragmentation patterns.
31152228	2	21	theme	sulfation	353:361	arg1	isomers					372:378	specific sulfation position isomers	344:378	specific sulfation position isomers	344:378	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	10	22	theme	larger	1398:1403	arg1	library					1414:1420	our larger fragment library	1394:1420	our larger fragment library	1394:1420	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	1	23	theme	important	206:214	arg1	processes					227:235	critically important biological processes	195:235	critically important biological processes	195:235	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	5	24	theme	sequence	857:864	arg1	inclusive					866:874	sequence inclusive	857:874	sequence inclusive of all sulfation sites	857:897	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	6	25	theme	GAG	1007:1009	arg1	Arixtra®					1021:1028	Arixtra®	1021:1028	Arixtra®	1021:1028	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	6	25	theme	GAG	1007:1009	arg1	pentamer					1011:1018	a synthetic GAG pentamer	995:1018	a synthetic GAG pentamer	995:1018	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	2	26	theme	specific	344:351	arg1	isomers					372:378	specific sulfation position isomers	344:378	specific sulfation position isomers	344:378	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	1	27	theme	biological	216:225	arg1	processes					227:235	critically important biological processes	195:235	critically important biological processes	195:235	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	0	28	theme	spatial	14:20	arg1	resolution					22:31	MSn spatial resolution	10:31	MSn spatial resolution	10:31	Advancing MSn spatial resolution and documentation for glycosaminoglycans by sulfate-isotope exchange.
31152228	4	29	theme	chemistries	623:633	arg1	set					616:618	A set	614:618	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation	614:720	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	4	29	theme	chemistries	623:633	arg1	chemistries					623:633	chemistries	623:633	chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation	623:720	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	7	30	theme	individual	1126:1135	arg1	positions					1147:1155	individual sulfation positions	1126:1155	individual sulfation positions	1126:1155	Upon disassembly, glycosidic and cross-ring cleavages define the monomer composition including individual sulfation positions.
31152228	1	31	theme	processes	227:235	arg1	host					187:190	a host	185:190	a host of critically important biological processes	185:235	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	3	32	theme	isotope	460:466	arg1	exchange					468:475	specific isotope exchange	451:475	specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization	451:611	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	10	33	from	15 years	1428:1435	arg1	the					1440:1442	the	1440:1442	the	1440:1442	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	7	34	theme	monomer	1096:1102	arg1	composition					1104:1114	the monomer composition	1092:1114	the monomer composition including individual sulfation positions	1092:1155	Upon disassembly, glycosidic and cross-ring cleavages define the monomer composition including individual sulfation positions.
31152228	6	35	theme	documenting	903:913	arg1	modifications					948:960	these chemical modifications	933:960	these chemical modifications	933:960	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	6	35	theme	documenting	903:913	arg1	steps					915:919	documenting steps	903:919	documenting steps	903:919	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	4	36	dep	replacement	693:703	arg1	CD3-CO-					684:690	CD3-CO-	684:690	CD3-CO-	684:690	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	4	36	dep	replacement	693:703	arg1	CD3-					675:678	CD3-	675:678	CD3-	675:678	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	5	37	theme	sulfation	883:891	arg1	sites					893:897	all sulfation sites	879:897	all sulfation sites	879:897	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	7	38	theme	glycosidic	1049:1058	arg1	cleavages					1075:1083	glycosidic and cross-ring cleavages	1049:1083	glycosidic and cross-ring cleavages	1049:1083	Upon disassembly, glycosidic and cross-ring cleavages define the monomer composition including individual sulfation positions.
31152228	5	39	theme	sites	893:897	arg1	inclusive					866:874	sequence inclusive	857:874	sequence inclusive of all sulfation sites	857:897	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	6	40	theme	disaccharides	977:989	arg1	series					967:972	a series	965:972	a series of disaccharides and a synthetic GAG pentamer, Arixtra®	965:1028	As documenting steps, we applied these chemical modifications to a series of disaccharides and a synthetic GAG pentamer, Arixtra®.
31152228	4	41	theme	stable	752:757	arg1	product					759:765	a stable product	750:765	a stable product ideal for sequencing by MSn	750:793	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	3	42	theme	specific	451:458	arg1	exchange					468:475	specific isotope exchange	451:475	specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization	451:611	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	2	43	theme	significant	240:250	arg1	determination					327:339	the determination	323:339	the determination of specific sulfation position isomers	323:378	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	2	43	theme	significant	240:250	arg1	difficulty					252:261	A significant difficulty	238:261	A significant difficulty in the comprehensive structural characterization of GAGs	238:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	1	44	theme	carbohydrate	133:144	arg1	polymers					156:163	carbohydrate polyionic polymers	133:163	carbohydrate polyionic polymers that participate in a host of critically important biological processes	133:235	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	1	44	theme	carbohydrate	133:144	arg1	Glycosaminoglycans					103:120	Glycosaminoglycans	103:120	Glycosaminoglycans (GAGs)	103:127	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	7	45	theme	cross-ring	1064:1073	arg1	cleavages					1075:1083	glycosidic and cross-ring cleavages	1049:1083	glycosidic and cross-ring cleavages	1049:1083	Upon disassembly, glycosidic and cross-ring cleavages define the monomer composition including individual sulfation positions.
31152228	10	46	theme	fragment	1405:1412	arg1	library					1414:1420	our larger fragment library	1394:1420	our larger fragment library	1394:1420	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	1	47	theme	polyionic	146:154	arg1	polymers					156:163	carbohydrate polyionic polymers	133:163	carbohydrate polyionic polymers that participate in a host of critically important biological processes	133:235	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	1	47	theme	polyionic	146:154	arg1	Glycosaminoglycans					103:120	Glycosaminoglycans	103:120	Glycosaminoglycans (GAGs)	103:127	Glycosaminoglycans (GAGs) are carbohydrate polyionic polymers that participate in a host of critically important biological processes.
31152228	10	48	theme	fragment	1323:1330	arg1	library					1332:1338	A fragment library	1321:1338	A fragment library of these products	1321:1356	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	10	48	theme	fragment	1323:1330	arg1	adjunct					1383:1389	an adjunct	1380:1389	an adjunct to our larger fragment library	1380:1420	A fragment library of these products is being assembled as an adjunct to our larger fragment library, some 15 years in the making.
31152228	4	49	theme	isotopic	665:672	arg1	replacement					693:703	isotopic (CD3- and CD3-CO-) replacement	665:703	isotopic (CD3- and CD3-CO-) replacement	665:703	A set of chemistries that include sulfate release, isotopic (CD3- and CD3-CO-) replacement, and methylation have been modified to yield a stable product ideal for sequencing by MSn.
31152228	3	50	theme	structural	585:594	arg1	characterization					596:611	a detailed structural characterization	574:611	a detailed structural characterization	574:611	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	5	51	theme	inclusive	866:874	arg1	read-out					845:852	a detailed read-out	834:852	a detailed read-out of sequence inclusive of all sulfation sites	834:897	Disassembly of these samples provides a detailed read-out of sequence inclusive of all sulfation sites.
31152228	2	52	theme	isomers	372:378	arg1	determination					327:339	the determination	323:339	the determination of specific sulfation position isomers	323:378	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	2	52	theme	isomers	372:378	arg1	difficulty					252:261	A significant difficulty	238:261	A significant difficulty in the comprehensive structural characterization of GAGs	238:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	3	53	theme	induced	528:534	arg1	dissociation					536:547	collisional induced dissociation	516:547	collisional induced dissociation	516:547	We chose to circumvent sulfate lability by its liberation followed by specific isotope exchange that makes it amenable to methylation, collisional induced dissociation, and MSn disassembly for a detailed structural characterization.
31152228	0	54	theme	sulfate-isotope	77:91	arg1	exchange					93:100	sulfate-isotope exchange	77:100	sulfate-isotope exchange	77:100	Advancing MSn spatial resolution and documentation for glycosaminoglycans by sulfate-isotope exchange.
31152228	2	55	theme	structural	284:293	arg1	characterization					295:310	the comprehensive structural characterization	266:310	the comprehensive structural characterization of GAGs	266:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31152228	2	56	theme	comprehensive	270:282	arg1	characterization					295:310	the comprehensive structural characterization	266:310	the comprehensive structural characterization of GAGs	266:318	A significant difficulty in the comprehensive structural characterization of GAGs is the determination of specific sulfation position isomers.
31739069	5	0	theme	-β-d-Glcp-	860:869	arg1	 → backbone					871:881	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	 → backbone	871:881	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	5	1	theme	Smith	764:768	arg1	degradation					770:780	Smith degradation	764:780	Smith degradation	764:780	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	0	2	from	structure	9:17	arg1	α-glucosidase					37:49	α-glucosidase	37:49	α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue	37:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	5	3	theme	-α-d-Galp-	948:957	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	9	4	theme	inhibition	1453:1462	arg1	activity					1464:1471	certain inhibition activity	1445:1471	certain inhibition activity	1445:1471	Furthermore, the AGP exhibited certain inhibition activity on α-glucosidase.
31739069	1	5	theme	DEAE-celluloseand	252:268	arg1	G-150					279:283	DEAE-celluloseand Sephadex G-150	252:283	DEAE-celluloseand Sephadex G-150	252:283	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	3	6	theme	AGP	454:456	arg1	rotation					442:449	Thespecific rotation	430:449	Thespecific rotation of AGP	430:456	Thespecific rotation of AGP was detected by a polarimeter and it was +45°.
31739069	8	7	theme	crystal	1305:1311	arg1	configuration					1313:1325	The crystal configuration	1301:1325	The crystal configuration	1301:1325	The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure.
31739069	9	8	theme	certain	1445:1451	arg1	activity					1464:1471	certain inhibition activity	1445:1471	certain inhibition activity	1445:1471	Furthermore, the AGP exhibited certain inhibition activity on α-glucosidase.
31739069	7	9	theme	thin	1255:1258	arg1	"					1265:1265	a "thin slice"	1252:1265	a "thin slice"	1252:1265	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	7	9	theme	thin	1255:1258	arg1	shape					1235:1239	the shape	1231:1239	the shape of AGP	1231:1246	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	1	10	theme	Sephadex	270:277	arg1	G-150					279:283	DEAE-celluloseand Sephadex G-150	252:283	DEAE-celluloseand Sephadex G-150	252:283	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	0	11	theme	inflata	110:116	arg1	residue					118:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	5	12	theme	-α-Manp-	968:975	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	6	13	theme	experiment	1035:1044	arg1	results					1014:1020	The results	1010:1020	The results of Congo red experiment and circular dichroism (CD)	1010:1072	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	2	14	theme	single	288:293	arg1	peak					311:314	A single and symmetrical peak	286:314	A single and symmetrical peak	286:314	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	5	15	theme	-α-l-Araf-	912:921	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	3	16	theme	Thespecific	430:440	arg1	rotation					442:449	Thespecific rotation	430:449	Thespecific rotation of AGP	430:456	Thespecific rotation of AGP was detected by a polarimeter and it was +45°.
31739069	5	17	theme	1→ and	977:982	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	6	18	theme	helix	1103:1107	arg1	conformation					1109:1120	triple helix conformation	1096:1120	triple helix conformation	1096:1120	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	7	19	theme	electron	1183:1190	arg1	SEM					1204:1206	SEM	1204:1206	SEM	1204:1206	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	7	19	theme	electron	1183:1190	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy (SEM)	1174:1207	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	5	20	theme	→1	984:985	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	6	21	theme	triple	1096:1101	arg1	conformation					1109:1120	triple helix conformation	1096:1120	triple helix conformation	1096:1120	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	5	22	theme	periodate	743:751	arg1	oxidation					753:761	periodate oxidation	743:761	periodate oxidation	743:761	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	5	23	theme	-α-d-Xylp-	894:903	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	1	24	theme	glycyrrhiza	187:197	arg1	residue					199:205	glycyrrhiza residue	187:205	glycyrrhiza residue	187:205	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	0	25	theme	Chemical	0:7	arg1	structure					9:17	Chemical structure	0:17	Chemical structure	0:17	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	7	26	theme	AGP	1244:1246	arg1	shape					1235:1239	the shape	1231:1239	the shape of AGP	1231:1246	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	7	26	theme	AGP	1244:1246	arg1	"					1265:1265	a "thin slice"	1252:1265	a "thin slice"	1252:1265	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	2	27	theme	homogeneous	360:370	arg1	AGP					351:353	AGP	351:353	AGP	351:353	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	2	27	theme	homogeneous	360:370	arg1	polysaccharide					372:385	a homogeneous polysaccharide	358:385	a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa	358:427	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	7	28	theme	AGP	1153:1155	arg1	micro-structure					1134:1148	The micro-structure	1130:1148	The micro-structure of AGP	1130:1155	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	2	29	theme	KDa	425:427	arg1	weight					404:409	a molecular weight	392:409	a molecular weight of 2.89 × 103 KDa	392:427	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	6	30	theme	red	1031:1033	arg1	experiment					1035:1044	Congo red experiment	1025:1044	Congo red experiment	1025:1044	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	0	31	from	glycyrrhiza	98:108	arg1	alkaline-extracted					74:91	alkaline-extracted	74:91	alkaline-extracted	74:91	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	2	32	theme	symmetrical	299:309	arg1	peak					311:314	A single and symmetrical peak	286:314	A single and symmetrical peak	286:314	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	1	33	theme	5	210:210	arg1	%					211:211	%	211:211	%	211:211	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	5	34	theme	AGP	716:718	arg1	structure					703:711	The structure	699:711	The structure of AGP	699:718	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	5	35	theme	-α-l-Rhap-	930:939	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	4	36	theme	molar	656:660	arg1	ratio					662:666	a molar ratio	654:666	a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	654:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	1	37	theme	%	211:211	arg1	solution					227:234	5% NaOH alkaline solution	210:234	5% NaOH alkaline solution	210:234	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	7	38	theme	scanning	1174:1181	arg1	SEM					1204:1206	SEM	1204:1206	SEM	1204:1206	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	7	38	theme	scanning	1174:1181	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy (SEM)	1174:1207	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	4	39	theme	composition	520:530	arg1	analysis					532:539	Monosaccharide composition analysis	505:539	Monosaccharide composition analysis	505:539	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	4	40	dep	l-rhamnose	577:586	arg1	l-arabinose					589:599	l-arabinose	589:599	l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	577:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	4	40	dep	l-rhamnose	577:586	arg1	d-xylose					602:609	d-xylose	602:609	l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	577:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	4	40	dep	l-rhamnose	577:586	arg1	d-galactose					637:647	d-galactose	637:647	d-galactose	637:647	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	4	40	dep	l-rhamnose	577:586	arg1	d-glucose					623:631	d-glucose	623:631	d-glucose	623:631	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	4	40	dep	l-rhamnose	577:586	arg1	d-mannose					612:620	d-mannose	612:620	l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	577:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	1	41	theme	NaOH	213:216	arg1	solution					227:234	5% NaOH alkaline solution	210:234	5% NaOH alkaline solution	210:234	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	0	42	theme	polysaccharide	54:67	arg1	α-glucosidase					37:49	α-glucosidase	37:49	α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue	37:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	4	43	theme	Monosaccharide	505:518	arg1	analysis					532:539	Monosaccharide composition analysis	505:539	Monosaccharide composition analysis	505:539	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	7	44	theme	slice	1260:1264	arg1	"					1265:1265	a "thin slice"	1252:1265	a "thin slice"	1252:1265	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	7	44	theme	slice	1260:1264	arg1	shape					1235:1239	the shape	1231:1239	the shape of AGP	1231:1246	The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular.
31739069	1	45	theme	new	129:131	arg1	polysaccharide					141:154	A new neutral polysaccharide	127:154	A new neutral polysaccharide	127:154	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	8	46	theme	X-ray	1345:1349	arg1	XRD					1364:1366	XRD	1364:1366	XRD	1364:1366	The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure.
31739069	8	46	theme	X-ray	1345:1349	arg1	diffraction					1351:1361	X-ray diffraction	1345:1361	X-ray diffraction (XRD)	1345:1367	The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure.
31739069	2	47	with	polysaccharide	372:385	arg1	weight					404:409	a molecular weight	392:409	a molecular weight of 2.89 × 103 KDa	392:427	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	4	48	with	d-galactose	637:647	arg1	ratio					662:666	a molar ratio	654:666	a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	654:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	1	49	theme	neutral	133:139	arg1	polysaccharide					141:154	A new neutral polysaccharide	127:154	A new neutral polysaccharide	127:154	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	5	50	theme	the →4	887:892	arg1	-β-d-Glcp					987:995	→ 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp	856:995	-β-d-Glcp	987:995	The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-( → backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches.
31739069	1	51	theme	alkaline	218:225	arg1	solution					227:234	5% NaOH alkaline solution	210:234	5% NaOH alkaline solution	210:234	A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150.
31739069	4	52	with	d-glucose	623:631	arg1	ratio					662:666	a molar ratio	654:666	a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	654:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	0	53	theme	alkaline-extracted	74:91	arg1	residue					118:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	2	54	theme	molecular	394:402	arg1	weight					404:409	a molecular weight	392:409	a molecular weight of 2.89 × 103 KDa	392:427	A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa.
31739069	6	55	theme	dichroism	1059:1067	arg1	results					1014:1020	The results	1010:1020	The results of Congo red experiment and circular dichroism (CD)	1010:1072	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	4	56	theme	1:2.33:2.85:0.69:3.05:1.54	671:696	arg1	ratio					662:666	a molar ratio	654:666	a molar ratio of 1:2.33:2.85:0.69:3.05:1.54	654:696	Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54.
31739069	0	57	from	inhibition	23:32	arg1	α-glucosidase					37:49	α-glucosidase	37:49	α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue	37:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	6	58	theme	circular	1050:1057	arg1	CD					1070:1071	CD	1070:1071	CD	1070:1071	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	6	58	theme	circular	1050:1057	arg1	dichroism					1059:1067	circular dichroism	1050:1067	circular dichroism (CD)	1050:1072	The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP.
31739069	0	59	with	α-glucosidase	37:49	arg1	residue					118:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	alkaline-extracted from glycyrrhiza inflata residue	74:124	Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
31739069	8	60	theme	crystal	1395:1401	arg1	structure					1403:1411	no crystal structure	1392:1411	no crystal structure	1392:1411	The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure.
31381913	3	0	theme	fractions	346:354	arg1	composition					291:301	monosaccharide composition	276:301	monosaccharide composition	276:301	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	0	theme	fractions	346:354	arg1	characteristics					318:332	structural characteristics	307:332	structural characteristics	307:332	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	1	dep	composition	291:301	arg1	The					272:274	The	272:274	The	272:274	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	2	theme	resonance	509:517	arg1	spectroscopy					519:530	nuclear magnetic resonance spectroscopy	492:530	nuclear magnetic resonance spectroscopy (NMR)	492:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	2	theme	resonance	509:517	arg1	NMR					533:535	NMR	533:535	NMR	533:535	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	4	3	theme	Saccharina	592:601	arg1	sculpera					603:610	Saccharina sculpera	592:610	Saccharina sculpera cultured in northern China	592:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	8	4	theme	HMG-CoA-R	1339:1347	arg1	downregulation					1321:1334	downregulation	1321:1334	downregulation of HMG-CoA-R	1321:1347	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	8	4	theme	HMG-CoA-R	1339:1347	arg1	upregulation					1353:1364	upregulation	1353:1364	upregulation of LCAT	1353:1372	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	7	5	theme	HDL-C	1133:1137	arg1	levels					1139:1144	HDL-C levels	1133:1144	HDL-C levels	1133:1144	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	4	6	theme	gene	724:727	arg1	expression					734:743	hepatic gene mRNA expression	716:743	hepatic gene mRNA expression	716:743	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	8	7	theme	gene	1244:1247	arg1	expression					1249:1258	hepatic gene expression	1236:1258	hepatic gene expression	1236:1258	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	8	8	theme	LPL	1517:1519	arg1	upregulation					1484:1495	upregulation	1484:1495	upregulation of PPARα, PPARγ and LPL	1484:1519	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	8	9	theme	SREBP-1c	1431:1438	arg1	downregulation					1413:1426	downregulation	1413:1426	downregulation of SREBP-1c	1413:1438	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	5	10	theme	glucuronic	911:920	arg1	acid					922:925	glucuronic acid	911:925	glucuronic acid	911:925	The results showed that F1 is an acetylated galactofucan and that F2 consists of fucose, galactose, mannose and glucuronic acid.
31381913	8	11	theme	fatty	1397:1401	arg1	acids					1403:1407	fatty acids	1397:1407	fatty acids	1397:1407	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	4	12	from	sculpera	603:610	arg1	effects					557:563	The hypolipidemic effects	539:563	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China	539:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	4	12	from	sculpera	603:610	arg1	fractions					577:585	fucoidan fractions	568:585	fucoidan fractions from Saccharina sculpera cultured in northern China	568:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	0	13	from	sculpera	60:67	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).	0:97	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).
31381913	8	14	theme	LCAT	1369:1372	arg1	downregulation					1321:1334	downregulation	1321:1334	downregulation of HMG-CoA-R	1321:1347	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	8	14	theme	LCAT	1369:1372	arg1	upregulation					1353:1364	upregulation	1353:1364	upregulation of LCAT	1353:1372	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	4	15	theme	mRNA	729:732	arg1	expression					734:743	hepatic gene mRNA expression	716:743	hepatic gene mRNA expression	716:743	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	9	16	theme	fatty	1689:1693	arg1	synthesis					1700:1708	fatty acid synthesis	1689:1708	fatty acid synthesis	1689:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	7	17	theme	Fucoidan	979:986	arg1	fractions					988:996	Fucoidan fractions	979:996	Fucoidan fractions from Saccharina sculpera	979:1021	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	7	18	from	accumulation	1182:1193	arg1	liver					1202:1206	the liver	1198:1206	the liver	1198:1206	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	6	19	with	galactofucan	948:959	arg1	fucose					971:976	high fucose	966:976	high fucose	966:976	F3 is an acetylated galactofucan with high fucose.
31381913	0	20	theme	Hypolipidemic	0:12	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).	0:97	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).
31381913	1	21	theme	hypolipidemic	173:185	arg1	activity					187:194	hypolipidemic activity	173:194	hypolipidemic activity	173:194	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31381913	5	22	theme	acetylated	832:841	arg1	galactofucan					843:854	an acetylated galactofucan	829:854	an acetylated galactofucan	829:854	The results showed that F1 is an acetylated galactofucan and that F2 consists of fucose, galactose, mannose and glucuronic acid.
31381913	5	22	theme	acetylated	832:841	arg1	F1					823:824	F1	823:824	F1	823:824	The results showed that F1 is an acetylated galactofucan and that F2 consists of fucose, galactose, mannose and glucuronic acid.
31381913	9	23	theme	synthesis	1700:1708	arg1	regulation					1675:1684	the regulation	1671:1684	the regulation of fatty acid synthesis	1671:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	23	theme	synthesis	1700:1708	arg1	inhibition					1612:1621	the inhibition	1608:1621	the inhibition of cholesterol synthesis and reverse transport	1608:1668	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	23	theme	synthesis	1700:1708	arg1	acceleration					1715:1726	acceleration	1715:1726	acceleration of mitochondrial β-oxidation	1715:1755	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	4	24	theme	Wistar	781:786	arg1	model					792:796	an established hyperlipidemic Wistar rat model	751:796	an established hyperlipidemic Wistar rat model	751:796	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	3	25	theme	high-performance	394:409	arg1	Fourier					441:447	Fourier	441:447	Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	441:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	25	theme	high-performance	394:409	arg1	chromatography					418:431	high-performance liquid chromatography	394:431	high-performance liquid chromatography (HPLC)	394:438	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	25	theme	high-performance	394:409	arg1	HPLC					434:437	HPLC	434:437	HPLC	434:437	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	9	26	theme	fucoidan	1584:1591	arg1	activity					1572:1579	the hypolipidemic activity	1554:1579	the hypolipidemic activity of fucoidan	1554:1591	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	26	theme	fucoidan	1584:1591	arg1	related					1597:1603	related	1597:1603	related	1597:1603	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	8	27	theme	PPARα	1500:1504	arg1	upregulation					1484:1495	upregulation	1484:1495	upregulation of PPARα, PPARγ and LPL	1484:1519	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	4	28	theme	hyperlipidemic	766:779	arg1	model					792:796	an established hyperlipidemic Wistar rat model	751:796	an established hyperlipidemic Wistar rat model	751:796	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	7	29	theme	TG	1093:1094	arg1	TC					1097:1098	the TG, TC, and LDL-C levels	1089:1116	TC	1097:1098	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	4	30	theme	antioxidative	687:699	arg1	indicators					701:710	antioxidative indicators	687:710	antioxidative indicators	687:710	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	0	31	theme	fucoidan	25:32	arg1	fractions					34:42	fucoidan fractions	25:42	fucoidan fractions	25:42	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).
31381913	3	32	dep	fractions	346:354	arg1	fractions					346:354	isolated fractions	337:354	isolated fractions (F1, F2 and F3)	337:370	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	32	dep	fractions	346:354	arg1	F3					368:369	F3	368:369	F3	368:369	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	32	dep	fractions	346:354	arg1	F2					361:362	F2	361:362	F2	361:362	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	9	33	theme	cholesterol	1626:1636	arg1	synthesis					1638:1646	cholesterol synthesis	1626:1646	cholesterol synthesis	1626:1646	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	8	34	theme	acids	1403:1407	arg1	synthesis					1384:1392	the synthesis	1380:1392	the synthesis of fatty acids	1380:1407	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	2	35	from	rich	254:257	arg1	fucoidan					262:269	fucoidan	262:269	fucoidan	262:269	Saccharina sculpera has attracted interest because it is rich in fucoidan.
31381913	4	36	from	effects	557:563	arg1	sculpera					603:610	Saccharina sculpera	592:610	Saccharina sculpera cultured in northern China	592:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	4	37	theme	fractions	577:585	arg1	effects					557:563	The hypolipidemic effects	539:563	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China	539:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	8	38	theme	fatty	1468:1472	arg1	acids					1474:1478	fatty acids	1468:1478	fatty acids	1468:1478	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	4	39	theme	rat	788:790	arg1	model					792:796	an established hyperlipidemic Wistar rat model	751:796	an established hyperlipidemic Wistar rat model	751:796	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	4	40	theme	hepatic	716:722	arg1	expression					734:743	hepatic gene mRNA expression	716:743	hepatic gene mRNA expression	716:743	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	4	41	theme	fucoidan	568:575	arg1	fractions					577:585	fucoidan fractions	568:585	fucoidan fractions from Saccharina sculpera cultured in northern China	568:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	1	42	link	algae-derived	127:139	arg1	macromolecule					141:153	brown algae-derived macromolecule	121:153	brown algae-derived macromolecule	121:153	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31381913	8	43	theme	cholesterol	1295:1305	arg1	synthesis					1307:1315	cholesterol synthesis	1295:1315	cholesterol synthesis	1295:1315	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	9	44	theme	acid	1695:1698	arg1	synthesis					1700:1708	fatty acid synthesis	1689:1708	fatty acid synthesis	1689:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	2	45	from	fucoidan	262:269	arg1	rich					254:257	rich	254:257	rich	254:257	Saccharina sculpera has attracted interest because it is rich in fucoidan.
31381913	4	46	theme	cholesterol	667:677	arg1	levels					679:684	cholesterol levels	667:684	cholesterol levels	667:684	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	0	47	theme	fractions	34:42	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).	0:97	Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae).
31381913	3	48	theme	monosaccharide	276:289	arg1	composition					291:301	monosaccharide composition	276:301	monosaccharide composition	276:301	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	49	theme	liquid	411:416	arg1	Fourier					441:447	Fourier	441:447	Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	441:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	49	theme	liquid	411:416	arg1	chromatography					418:431	high-performance liquid chromatography	394:431	high-performance liquid chromatography (HPLC)	394:438	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	49	theme	liquid	411:416	arg1	HPLC					434:437	HPLC	434:437	HPLC	434:437	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	6	50	theme	high	966:969	arg1	fucose					971:976	high fucose	966:976	high fucose	966:976	F3 is an acetylated galactofucan with high fucose.
31381913	9	51	theme	β-oxidation	1745:1755	arg1	regulation					1675:1684	the regulation	1671:1684	the regulation of fatty acid synthesis	1671:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	51	theme	β-oxidation	1745:1755	arg1	inhibition					1612:1621	the inhibition	1608:1621	the inhibition of cholesterol synthesis and reverse transport	1608:1668	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	51	theme	β-oxidation	1745:1755	arg1	acceleration					1715:1726	acceleration	1715:1726	acceleration of mitochondrial β-oxidation	1715:1755	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	8	52	theme	acids	1474:1478	arg1	β-oxidation					1453:1463	β-oxidation	1453:1463	β-oxidation of fatty acids	1453:1478	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	3	53	dep	Fourier	441:447	arg1	transform					449:457	transform	449:457	transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	449:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	7	54	theme	lipids	1061:1066	arg1	level					1052:1056	the level	1048:1056	the level of lipids	1048:1066	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	3	55	theme	nuclear	492:498	arg1	spectroscopy					519:530	nuclear magnetic resonance spectroscopy	492:530	nuclear magnetic resonance spectroscopy (NMR)	492:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	55	theme	nuclear	492:498	arg1	NMR					533:535	NMR	533:535	NMR	533:535	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	9	56	theme	mitochondrial	1731:1743	arg1	β-oxidation					1745:1755	mitochondrial β-oxidation	1731:1755	mitochondrial β-oxidation	1731:1755	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	4	57	theme	hypolipidemic	543:555	arg1	effects					557:563	The hypolipidemic effects	539:563	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China	539:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	3	58	theme	isolated	337:344	arg1	fractions					346:354	isolated fractions	337:354	isolated fractions (F1, F2 and F3)	337:370	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	58	theme	isolated	337:344	arg1	F3					368:369	F3	368:369	F3	368:369	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	58	theme	isolated	337:344	arg1	F2					361:362	F2	361:362	F2	361:362	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	59	dep	transform	449:457	arg1	infrared					459:466	infrared	459:466	transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR)	449:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	60	theme	magnetic	500:507	arg1	spectroscopy					519:530	nuclear magnetic resonance spectroscopy	492:530	nuclear magnetic resonance spectroscopy (NMR)	492:536	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	3	60	theme	magnetic	500:507	arg1	NMR					533:535	NMR	533:535	NMR	533:535	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	9	61	theme	transport	1660:1668	arg1	regulation					1675:1684	the regulation	1671:1684	the regulation of fatty acid synthesis	1671:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	61	theme	transport	1660:1668	arg1	inhibition					1612:1621	the inhibition	1608:1621	the inhibition of cholesterol synthesis and reverse transport	1608:1668	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	61	theme	transport	1660:1668	arg1	acceleration					1715:1726	acceleration	1715:1726	acceleration of mitochondrial β-oxidation	1715:1755	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	3	62	theme	structural	307:316	arg1	characteristics					318:332	structural characteristics	307:332	structural characteristics	307:332	The monosaccharide composition and structural characteristics of isolated fractions (F1, F2 and F3) were determined using high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR).
31381913	9	63	theme	synthesis	1638:1646	arg1	regulation					1675:1684	the regulation	1671:1684	the regulation of fatty acid synthesis	1671:1708	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	63	theme	synthesis	1638:1646	arg1	inhibition					1612:1621	the inhibition	1608:1621	the inhibition of cholesterol synthesis and reverse transport	1608:1668	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	63	theme	synthesis	1638:1646	arg1	acceleration					1715:1726	acceleration	1715:1726	acceleration of mitochondrial β-oxidation	1715:1755	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	4	64	theme	established	754:764	arg1	model					792:796	an established hyperlipidemic Wistar rat model	751:796	an established hyperlipidemic Wistar rat model	751:796	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	4	65	theme	northern	624:631	arg1	China					633:637	northern China	624:637	northern China	624:637	The hypolipidemic effects of fucoidan fractions from Saccharina sculpera cultured in northern China were clarified by measuring cholesterol levels, antioxidative indicators and hepatic gene mRNA expression using an established hyperlipidemic Wistar rat model.
31381913	8	66	dep	downregulation	1321:1334	arg1	slow					1375:1378	slow	1375:1378	slow	1375:1378	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	7	67	theme	LDL-C	1105:1109	arg1	levels					1111:1116	the TG, TC, and LDL-C levels	1089:1116	levels	1111:1116	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	8	68	theme	PPARγ	1507:1511	arg1	upregulation					1484:1495	upregulation	1484:1495	upregulation of PPARα, PPARγ and LPL	1484:1519	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	1	69	theme	brown	121:125	arg1	macromolecule					141:153	brown algae-derived macromolecule	121:153	brown algae-derived macromolecule	121:153	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31381913	2	70	theme	Saccharina	197:206	arg1	sculpera					208:215	Saccharina sculpera	197:215	Saccharina sculpera	197:215	Saccharina sculpera has attracted interest because it is rich in fucoidan.
31381913	9	71	theme	reverse	1652:1658	arg1	transport					1660:1668	reverse transport	1652:1668	reverse transport	1652:1668	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	1	72	theme	algae-derived	127:139	arg1	macromolecule					141:153	brown algae-derived macromolecule	121:153	brown algae-derived macromolecule	121:153	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31381913	7	73	from	sculpera	1014:1021	arg1	fractions					988:996	Fucoidan fractions	979:996	Fucoidan fractions from Saccharina sculpera	979:1021	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	9	74	theme	hypolipidemic	1558:1570	arg1	activity					1572:1579	the hypolipidemic activity	1554:1579	the hypolipidemic activity of fucoidan	1554:1591	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	9	74	theme	hypolipidemic	1558:1570	arg1	related					1597:1603	related	1597:1603	related	1597:1603	These results demonstrated that the hypolipidemic activity of fucoidan was related to the inhibition of cholesterol synthesis and reverse transport, the regulation of fatty acid synthesis, and acceleration of mitochondrial β-oxidation.
31381913	8	75	theme	hepatic	1236:1242	arg1	expression					1249:1258	hepatic gene expression	1236:1258	hepatic gene expression	1236:1258	The findings obtained from hepatic gene expression showed that fucoidan could inhibit cholesterol synthesis via downregulation of HMG-CoA-R and upregulation of LCAT, slow the synthesis of fatty acids via downregulation of SREBP-1c, and promote β-oxidation of fatty acids via upregulation of PPARα, PPARγ and LPL.
31381913	6	76	theme	acetylated	937:946	arg1	galactofucan					948:959	an acetylated galactofucan	934:959	an acetylated galactofucan with high fucose	934:976	F3 is an acetylated galactofucan with high fucose.
31381913	6	76	theme	acetylated	937:946	arg1	F3					928:929	F3	928:929	F3	928:929	F3 is an acetylated galactofucan with high fucose.
31381913	7	77	theme	lipid	1176:1180	arg1	accumulation					1182:1193	lipid accumulation	1176:1193	lipid accumulation in the liver	1176:1206	Fucoidan fractions from Saccharina sculpera could effectively reduce the level of lipids in serum by reducing the TG, TC, and LDL-C levels and increasing HDL-C levels and could effectively prevent lipid accumulation in the liver.
31381913	1	78	theme	macromolecule	141:153	arg1	Fucoidan					99:106	Fucoidan	99:106	Fucoidan	99:106	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31381913	1	78	theme	macromolecule	141:153	arg1	kind					113:116	a kind	111:116	a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity	111:194	Fucoidan is a kind of brown algae-derived macromolecule suggested to have hypolipidemic activity.
31276726	0	0	theme	cross-linker	75:86	arg1	length					88:93	cross-linker length	75:93	cross-linker length	75:93	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	1	1	theme	molar	166:170	arg1	masses					172:177	varying molar masses	158:177	varying molar masses	158:177	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	5	2	theme	stiffer	854:860	arg1	hydrogels					862:870	stiffer hydrogels	854:870	stiffer hydrogels	854:870	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	6	3	theme	aromatic	1113:1120	arg1	drug					1137:1140	a traceable aromatic biobased model drug	1101:1140	a traceable aromatic biobased model drug	1101:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	1	4	theme	masses	172:177	arg1	cross-linkers					240:252	cross-linkers	240:252	cross-linkers for furan-modified alginate chains	240:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	4	theme	masses	172:177	arg1	trismaleimides					140:153	trismaleimides	140:153	trismaleimides	140:153	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	4	theme	masses	172:177	arg1	bismaleimides					122:134	Various bismaleimides	114:134	Various bismaleimides	114:134	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	6	5	theme	traceable	1103:1111	arg1	drug					1137:1140	a traceable aromatic biobased model drug	1101:1140	a traceable aromatic biobased model drug	1101:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	7	6	theme	polyethylene	1304:1315	arg1	bis					1330:1332	polyethylene glycol-based bis	1304:1332	polyethylene glycol-based bis	1304:1332	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	4	7	theme	cross-linkers	700:712	arg1	use					679:681	The use	675:681	The use of the different cross-linkers	675:712	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	2	8	theme	hydrogels	395:403	arg1	preparation					380:390	the preparation	376:390	the preparation of hydrogels based on naturally occurring biomacromolecules	376:450	An environmentally friendly approach is described for the preparation of hydrogels based on naturally occurring biomacromolecules, without catalysts.
31276726	2	9	theme	environmentally	325:339	arg1	approach					350:357	An environmentally friendly approach	322:357	An environmentally friendly approach	322:357	An environmentally friendly approach is described for the preparation of hydrogels based on naturally occurring biomacromolecules, without catalysts.
31276726	3	10	theme	potential	640:648	arg1	applications					661:672	potential biomedical applications	640:672	potential biomedical applications	640:672	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	1	11	theme	Diels-Alder	293:303	arg1	reactions					311:319	Diels-Alder click reactions	293:319	Diels-Alder click reactions	293:319	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	0	12	theme	length	88:93	arg1	Effect					65:70	Effect	65:70	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.	0:112	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	7	13	theme	molar	1367:1371	arg1	masses					1373:1378	different molar masses	1357:1378	different molar masses	1357:1378	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	3	14	theme	rheological	569:579	arg1	properties					581:590	rheological properties	569:590	rheological properties	569:590	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	1	15	theme	click	305:309	arg1	reactions					311:319	Diels-Alder click reactions	293:319	Diels-Alder click reactions	293:319	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	3	16	theme	swelling	559:566	arg1	terms					550:554	terms	550:554	terms of swelling, rheological properties and drug-release efficiency	550:618	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	6	17	theme	composition	1187:1197	arg1	release					1078:1084	release	1078:1084	release of vanillin as a traceable aromatic biobased model drug	1078:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	6	17	theme	composition	1187:1197	arg1	function					1166:1173	a function	1164:1173	a function of hydrogel composition	1164:1197	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	3	18	theme	efficiency	609:618	arg1	terms					550:554	terms	550:554	terms of swelling, rheological properties and drug-release efficiency	550:618	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	3	19	theme	resulting	492:500	arg1	hydrogels					524:532	the resulting polysaccharides-based hydrogels	488:532	the resulting polysaccharides-based hydrogels	488:532	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	0	20	theme	functionality	99:111	arg1	Effect					65:70	Effect	65:70	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.	0:112	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	5	21	theme	hydrogel	1013:1020	arg1	network					1022:1028	the hydrogel network	1009:1028	the hydrogel network	1009:1028	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	5	22	theme	high	889:892	arg1	modulus					902:908	high storage modulus	889:908	high storage modulus	889:908	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	1	23	theme	chemical	180:187	arg1	architectures					189:201	chemical architectures	180:201	chemical architectures	180:201	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	3	24	theme	biomedical	650:659	arg1	applications					661:672	potential biomedical applications	640:672	potential biomedical applications	640:672	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	1	25	theme	architectures	189:201	arg1	cross-linkers					240:252	cross-linkers	240:252	cross-linkers for furan-modified alginate chains	240:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	25	theme	architectures	189:201	arg1	trismaleimides					140:153	trismaleimides	140:153	trismaleimides	140:153	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	25	theme	architectures	189:201	arg1	bismaleimides					122:134	Various bismaleimides	114:134	Various bismaleimides	114:134	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	5	26	theme	chain	922:926	arg1	length					928:933	the chain length	918:933	the chain length	918:933	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	6	27	theme	vanillin	1089:1096	arg1	release					1078:1084	release	1078:1084	release of vanillin as a traceable aromatic biobased model drug	1078:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	6	27	theme	vanillin	1089:1096	arg1	function					1166:1173	a function	1164:1173	a function of hydrogel composition	1164:1197	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	7	28	theme	first-time	1238:1247	arg1	alginates					1264:1272	the first-time furan-modified alginates	1234:1272	the first-time furan-modified alginates	1234:1272	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	0	29	link	cross-linked	32:43	arg1	hydrogels					54:62	click cross-linked alginate hydrogels	26:62	click cross-linked alginate hydrogels	26:62	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	2	30	theme	occurring	424:432	arg1	biomacromolecules					434:450	naturally occurring biomacromolecules	414:450	naturally occurring biomacromolecules	414:450	An environmentally friendly approach is described for the preparation of hydrogels based on naturally occurring biomacromolecules, without catalysts.
31276726	7	31	theme	glycol-based	1317:1328	arg1	bis					1330:1332	polyethylene glycol-based bis	1304:1332	polyethylene glycol-based bis	1304:1332	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	6	32	theme	hydrogel	1178:1185	arg1	composition					1187:1197	hydrogel composition	1178:1197	hydrogel composition	1178:1197	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	6	33	theme	biobased	1122:1129	arg1	drug					1137:1140	a traceable aromatic biobased model drug	1101:1140	a traceable aromatic biobased model drug	1101:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	1	34	theme	functionalities	207:221	arg1	cross-linkers					240:252	cross-linkers	240:252	cross-linkers for furan-modified alginate chains	240:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	34	theme	functionalities	207:221	arg1	trismaleimides					140:153	trismaleimides	140:153	trismaleimides	140:153	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	34	theme	functionalities	207:221	arg1	bismaleimides					122:134	Various bismaleimides	114:134	Various bismaleimides	114:134	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	7	35	with	hydrogels	1421:1429	arg1	behavior					1446:1453	different behavior	1436:1453	different behavior	1436:1453	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	2	36	theme	friendly	341:348	arg1	approach					350:357	An environmentally friendly approach	322:357	An environmentally friendly approach	322:357	An environmentally friendly approach is described for the preparation of hydrogels based on naturally occurring biomacromolecules, without catalysts.
31276726	7	37	theme	furan-modified	1249:1262	arg1	alginates					1264:1272	the first-time furan-modified alginates	1234:1272	the first-time furan-modified alginates	1234:1272	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	7	38	theme	different	1436:1444	arg1	behavior					1446:1453	different behavior	1436:1453	different behavior	1436:1453	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	0	39	theme	cross-linked	32:43	arg1	hydrogels					54:62	click cross-linked alginate hydrogels	26:62	click cross-linked alginate hydrogels	26:62	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	7	40	theme	advanced	1412:1419	arg1	hydrogels					1421:1429	advanced hydrogels	1412:1429	advanced hydrogels with different behavior	1412:1453	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	3	41	theme	hydrogels	524:532	arg1	behavior					476:483	The behavior	472:483	The behavior of the resulting polysaccharides-based hydrogels	472:532	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	0	42	theme	click	26:30	arg1	hydrogels					54:62	click cross-linked alginate hydrogels	26:62	click cross-linked alginate hydrogels	26:62	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	5	43	theme	storage	894:900	arg1	modulus					902:908	high storage modulus	889:908	high storage modulus	889:908	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	5	44	theme	trifunctional	826:838	arg1	cross-linkers					840:852	trifunctional cross-linkers	826:852	trifunctional cross-linkers	826:852	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	6	45	theme	model	1131:1135	arg1	drug					1137:1140	a traceable aromatic biobased model drug	1101:1140	a traceable aromatic biobased model drug	1101:1140	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	4	46	theme	mechanical	732:741	arg1	properties					743:752	the mechanical properties	728:752	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	0	47	theme	hydrogels	54:62	arg1	behavior					14:21	behavior	14:21	behavior	14:21	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	0	47	theme	hydrogels	54:62	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	6	48	theme	delivery	1055:1062	arg1	applications					1064:1075	drug delivery applications	1050:1075	drug delivery applications	1050:1075	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	1	49	theme	Various	114:120	arg1	cross-linkers					240:252	cross-linkers	240:252	cross-linkers for furan-modified alginate chains	240:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	49	theme	Various	114:120	arg1	trismaleimides					140:153	trismaleimides	140:153	trismaleimides	140:153	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	1	49	theme	Various	114:120	arg1	bismaleimides					122:134	Various bismaleimides	114:134	Various bismaleimides	114:134	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	0	50	theme	alginate	45:52	arg1	hydrogels					54:62	click cross-linked alginate hydrogels	26:62	click cross-linked alginate hydrogels	26:62	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	6	51	theme	drug	1050:1053	arg1	applications					1064:1075	drug delivery applications	1050:1075	drug delivery applications	1050:1075	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	4	52	dep	tuning	721:726	arg1	properties					743:752	the mechanical properties	728:752	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	3	53	theme	properties	581:590	arg1	terms					550:554	terms	550:554	terms of swelling, rheological properties and drug-release efficiency	550:618	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	7	54	dep	reacted	1279:1285	arg1	best					1207:1210	best	1207:1210	best	1207:1210	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	4	55	theme	hydrogels	804:812	arg1	behavior					788:795	the pulsatile swelling behavior	765:795	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	4	55	theme	hydrogels	804:812	arg1	tuning					721:726	tuning	721:726	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	7	56	with	bis	1330:1332	arg1	masses					1373:1378	different molar masses	1357:1378	different molar masses	1357:1378	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	7	56	with	bis	1330:1332	arg1	architectures					1384:1396	architectures	1384:1396	architectures	1384:1396	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	3	57	with	connection	624:633	arg1	applications					661:672	potential biomedical applications	640:672	potential biomedical applications	640:672	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	4	58	theme	pulsatile	769:777	arg1	behavior					788:795	the pulsatile swelling behavior	765:795	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	6	59	with	connection	1034:1043	arg1	applications					1064:1075	drug delivery applications	1050:1075	drug delivery applications	1050:1075	In connection with drug delivery applications, release of vanillin as a traceable aromatic biobased model drug was also monitored as a function of hydrogel composition.
31276726	4	60	theme	different	690:698	arg1	cross-linkers					700:712	the different cross-linkers	686:712	the different cross-linkers	686:712	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	5	61	theme	network	1022:1028	arg1	swelling					997:1004	the swelling	993:1004	the swelling of the hydrogel network	993:1028	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	5	62	theme	cross-linker	962:973	arg1	length					928:933	the chain length	918:933	the chain length	918:933	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	5	62	theme	cross-linker	962:973	arg1	composition					943:953	the composition	939:953	the composition of the cross-linker	939:973	When using trifunctional cross-linkers stiffer hydrogels were formed with high storage modulus whereas the chain length and the composition of the cross-linker clearly influence the swelling of the hydrogel network.
31276726	3	63	theme	drug-release	596:607	arg1	efficiency					609:618	drug-release efficiency	596:618	drug-release efficiency	596:618	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	7	64	with	trismaleimides	1337:1350	arg1	masses					1373:1378	different molar masses	1357:1378	different molar masses	1357:1378	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	7	64	with	trismaleimides	1337:1350	arg1	architectures					1384:1396	architectures	1384:1396	architectures	1384:1396	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	4	65	theme	swelling	779:786	arg1	behavior					788:795	the pulsatile swelling behavior	765:795	tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels	721:812	The use of the different cross-linkers allows tuning the mechanical properties as well as the pulsatile swelling behavior of the hydrogels.
31276726	3	66	theme	polysaccharides-based	502:522	arg1	hydrogels					524:532	the resulting polysaccharides-based hydrogels	488:532	the resulting polysaccharides-based hydrogels	488:532	The behavior of the resulting polysaccharides-based hydrogels was analyzed in terms of swelling, rheological properties and drug-release efficiency, in connection with potential biomedical applications.
31276726	1	67	theme	furan-modified	258:271	arg1	chains					282:287	furan-modified alginate chains	258:287	furan-modified alginate chains	258:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	0	68	dep	Synthesis	0:8	arg1	Effect					65:70	Effect	65:70	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.	0:112	Synthesis and behavior of click cross-linked alginate hydrogels: Effect of cross-linker length and functionality.
31276726	1	69	theme	varying	158:164	arg1	masses					172:177	varying molar masses	158:177	varying molar masses	158:177	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
31276726	7	70	theme	different	1357:1365	arg1	masses					1373:1378	different molar masses	1357:1378	different molar masses	1357:1378	To the best of our knowledge, for the first-time furan-modified alginates were reacted and studied with polyethylene glycol-based bis or trismaleimides with different molar masses and architectures, resulting in advanced hydrogels with different behavior.
31276726	1	71	theme	alginate	273:280	arg1	chains					282:287	furan-modified alginate chains	258:287	furan-modified alginate chains	258:287	Various bismaleimides and trismaleimides of varying molar masses, chemical architectures and functionalities were explored as cross-linkers for furan-modified alginate chains via Diels-Alder click reactions.
30064816	0	0	theme	chiral	92:97	arg1	phases					110:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases	50:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol	50:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	1	1	theme	identical	167:175	arg1	conditions					193:202	Seemingly identical chromatographic conditions	157:202	Seemingly identical chromatographic conditions	157:202	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	5	2	theme	notable	798:804	arg1	observation					806:816	The most notable observation	789:816	The most notable observation	789:816	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	2	theme	notable	798:804	arg1	access					830:835	the easy access	821:835	the easy access to markedly different states of the CSP in the same solvent mixture	821:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	1	3	theme	chromatographic	177:191	arg1	conditions					193:202	Seemingly identical chromatographic conditions	157:202	Seemingly identical chromatographic conditions	157:202	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	5	4	from	methanol	966:973	arg1	other					982:986	other	982:986	other	982:986	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	0	5	from	Hysteresis	0:9	arg1	phases					110:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases	50:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol	50:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	4	6	theme	elution	774:780	arg1	order					782:786	the enantiomer elution order	759:786	the enantiomer elution order	759:786	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30064816	0	7	theme	stationary	99:108	arg1	phases					110:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases	50:115	amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol	50:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	3	8	theme	realistic	571:579	arg1	alteration					494:503	An alteration	491:503	An alteration in the structure of the chiral stationary phase (CSP)	491:557	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	8	theme	realistic	571:579	arg1	reason					581:586	the only realistic reason	562:586	the only realistic reason behind the dissimilar selectivities in the same eluent	562:641	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	5	9	from	mixture	897:903	arg1	observation					806:816	The most notable observation	789:816	The most notable observation	789:816	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	9	from	mixture	897:903	arg1	access					830:835	the easy access	821:835	the easy access to markedly different states of the CSP in the same solvent mixture	821:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	3	10	theme	phase	547:551	arg1	structure					512:520	the structure	508:520	the structure of the chiral stationary phase (CSP)	508:557	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	11	theme	chiral	529:534	arg1	CSP					554:556	CSP	554:556	CSP	554:556	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	11	theme	chiral	529:534	arg1	phase					547:551	the chiral stationary phase	525:551	the chiral stationary phase (CSP)	525:557	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	5	12	theme	short	910:914	arg1	pretreatment					916:927	a short pretreatment	908:927	a short pretreatment with 2-propanol in one case and with methanol in the other	908:986	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	13	from	2-propanol	934:943	arg1	case					952:955	one case	948:955	one case	948:955	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	7	14	theme	chiral	1351:1356	arg1	interactions					1358:1369	chiral interactions	1351:1369	chiral interactions	1351:1369	From a theoretical point of view, this so-called hysteretic behavior poses another challenge to consider when modeling chiral interactions.
30064816	5	15	with	pretreatment	916:927	arg1	2-propanol					934:943	2-propanol	934:943	2-propanol in one case	934:955	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	15	with	pretreatment	916:927	arg1	methanol					966:973	methanol	966:973	methanol in the other	966:986	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	4	16	theme	enantiomer	763:772	arg1	order					782:786	the enantiomer elution order	759:786	the enantiomer elution order	759:786	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30064816	5	17	theme	same	884:887	arg1	mixture					897:903	the same solvent mixture	880:903	the same solvent mixture	880:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	3	18	theme	dissimilar	599:608	arg1	selectivities					610:622	the dissimilar selectivities	595:622	the dissimilar selectivities in the same eluent	595:641	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	19	from	selectivities	610:622	arg1	eluent					636:641	the same eluent	627:641	the same eluent	627:641	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	5	20	theme	solvent	889:895	arg1	mixture					897:903	the same solvent mixture	880:903	the same solvent mixture	880:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	0	21	theme	2-propanol	132:141	arg1	mixtures					120:127	mixtures	120:127	mixtures of 2-propanol and methanol	120:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	4	22	theme	order	782:786	arg1	reversal					747:754	a reversal	745:754	a reversal of the enantiomer elution order	745:786	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30064816	2	23	theme	eluent	469:474	arg1	composition					450:460	the composition	446:460	the composition of the eluent	446:474	Selectivities of structurally diverse enantiomer pairs depend on the direction from which the composition of the eluent is approached.
30064816	0	24	theme	retention	14:22	arg1	Hysteresis					0:9	Hysteresis	0:9	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.	0:155	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	1	25	from	columns	308:314	arg1	mixtures					319:326	mixtures	319:326	mixtures of 2-propanol and methanol	319:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	2	26	theme	pairs	405:409	arg1	Selectivities					356:368	Selectivities	356:368	Selectivities of structurally diverse enantiomer pairs	356:409	Selectivities of structurally diverse enantiomer pairs depend on the direction from which the composition of the eluent is approached.
30064816	6	27	theme	eluent	1149:1154	arg1	composition					1130:1140	the composition	1126:1140	the composition of the eluent	1126:1154	Repeatability strongly depends on the composition of the eluent and it is key to utilization and also to rationalization of the phenomenon.
30064816	7	28	theme	so-called	1271:1279	arg1	behavior					1292:1299	this so-called hysteretic behavior	1266:1299	this so-called hysteretic behavior	1266:1299	From a theoretical point of view, this so-called hysteretic behavior poses another challenge to consider when modeling chiral interactions.
30064816	1	29	theme	2-propanol	331:340	arg1	mixtures					319:326	mixtures	319:326	mixtures of 2-propanol and methanol	319:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	0	30	theme	methanol	147:154	arg1	mixtures					120:127	mixtures	120:127	mixtures of 2-propanol and methanol	120:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	5	31	theme	long-term	1062:1070	arg1	stability					1072:1080	long-term stability	1062:1080	long-term stability for both	1062:1089	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	6	32	theme	phenomenon	1220:1229	arg1	rationalization					1197:1211	rationalization	1197:1211	rationalization of the phenomenon	1197:1229	Repeatability strongly depends on the composition of the eluent and it is key to utilization and also to rationalization of the phenomenon.
30064816	0	33	theme	enantioselectivity	28:45	arg1	Hysteresis					0:9	Hysteresis	0:9	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.	0:155	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	5	34	from	CSP	873:875	arg1	mixture					897:903	the same solvent mixture	880:903	the same solvent mixture	880:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	3	35	theme	same	631:634	arg1	eluent					636:641	the same eluent	627:641	the same eluent	627:641	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	36	from	alteration	494:503	arg1	structure					512:520	the structure	508:520	the structure of the chiral stationary phase (CSP)	508:557	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	1	37	theme	amylose	258:264	arg1	3,5-dimethylphenylcarbamate					271:297	3,5-dimethylphenylcarbamate	271:297	3,5-dimethylphenylcarbamate	271:297	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	1	37	theme	amylose	258:264	arg1	ADMPC					301:305	ADMPC	301:305	ADMPC	301:305	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	1	37	theme	amylose	258:264	arg1	tris					266:269	amylose tris	258:269	amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol	258:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	1	38	theme	methanol	346:353	arg1	mixtures					319:326	mixtures	319:326	mixtures of 2-propanol and methanol	319:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	5	39	from	access	830:835	arg1	mixture					897:903	the same solvent mixture	880:903	the same solvent mixture	880:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	2	40	theme	enantiomer	394:403	arg1	pairs					405:409	structurally diverse enantiomer pairs	373:409	structurally diverse enantiomer pairs	373:409	Selectivities of structurally diverse enantiomer pairs depend on the direction from which the composition of the eluent is approached.
30064816	5	41	theme	easy	825:828	arg1	observation					806:816	The most notable observation	789:816	The most notable observation	789:816	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	41	theme	easy	825:828	arg1	access					830:835	the easy access	821:835	the easy access to markedly different states of the CSP in the same solvent mixture	821:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	7	42	theme	view	1260:1263	arg1	point					1251:1255	a theoretical point	1237:1255	a theoretical point of view	1237:1263	From a theoretical point of view, this so-called hysteretic behavior poses another challenge to consider when modeling chiral interactions.
30064816	2	43	theme	diverse	386:392	arg1	pairs					405:409	structurally diverse enantiomer pairs	373:409	structurally diverse enantiomer pairs	373:409	Selectivities of structurally diverse enantiomer pairs depend on the direction from which the composition of the eluent is approached.
30064816	1	44	from	enantioseparations	236:253	arg1	columns					308:314	amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns	258:314	amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol	258:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	1	45	theme	tris	266:269	arg1	columns					308:314	amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns	258:314	amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol	258:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	0	46	from	phases	110:115	arg1	mixtures					120:127	mixtures	120:127	mixtures of 2-propanol and methanol	120:154	Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol.
30064816	5	47	theme	different	849:857	arg1	states					859:864	markedly different states	840:864	markedly different states	840:864	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	7	48	theme	theoretical	1239:1249	arg1	point					1251:1255	a theoretical point	1237:1255	a theoretical point of view	1237:1263	From a theoretical point of view, this so-called hysteretic behavior poses another challenge to consider when modeling chiral interactions.
30064816	5	49	theme	CSP	873:875	arg1	observation					806:816	The most notable observation	789:816	The most notable observation	789:816	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	5	49	theme	CSP	873:875	arg1	access					830:835	the easy access	821:835	the easy access to markedly different states of the CSP in the same solvent mixture	821:903	The most notable observation is the easy access to markedly different states of the CSP in the same solvent mixture by a short pretreatment with 2-propanol in one case and with methanol in the other, while the transition between the two states is hindered enough to ensure long-term stability for both.
30064816	1	50	theme	different	226:234	arg1	enantioseparations					236:253	drastically different enantioseparations	214:253	drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol	214:353	Seemingly identical chromatographic conditions result in drastically different enantioseparations on amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) columns in mixtures of 2-propanol and methanol.
30064816	7	51	theme	hysteretic	1281:1290	arg1	behavior					1292:1299	this so-called hysteretic behavior	1266:1299	this so-called hysteretic behavior	1266:1299	From a theoretical point of view, this so-called hysteretic behavior poses another challenge to consider when modeling chiral interactions.
30064816	4	52	theme	recognition	676:686	arg1	mechanisms					688:697	History-dependent retention and recognition mechanisms	644:697	mechanisms	688:697	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30064816	4	53	theme	Hoff	722:725	arg1	plots					727:731	n't Hoff plots	718:731	n't Hoff plots	718:731	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30064816	3	54	theme	stationary	536:545	arg1	CSP					554:556	CSP	554:556	CSP	554:556	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	3	54	theme	stationary	536:545	arg1	phase					547:551	the chiral stationary phase	525:551	the chiral stationary phase (CSP)	525:557	An alteration in the structure of the chiral stationary phase (CSP) is the only realistic reason behind the dissimilar selectivities in the same eluent.
30064816	4	55	theme	History-dependent	644:660	arg1	retention					662:670	History-dependent retention and recognition mechanisms	644:697	retention	662:670	History-dependent retention and recognition mechanisms are indicated by van't Hoff plots and even by a reversal of the enantiomer elution order.
30534192	9	0	dep	reinhardtii	1347:1357	arg1	Chlamydomonas					1333:1345	the green microalgae, Chlamydomonas reinhardtii	1311:1357	Chlamydomonas	1333:1345	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	10	1	theme	linear	1636:1641	arg1	Glc3Man5GlcNAc2					1643:1657	a linear Glc3Man5GlcNAc2	1634:1657	a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1634:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	9	2	theme	tandem	1251:1256	arg1	spectrometry					1263:1274	multistage tandem mass spectrometry	1240:1274	multistage tandem mass spectrometry in two models of microalgae	1240:1302	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	6	3	link	N-linked	888:895	arg1	glycans					897:903	N-linked glycans	888:903	N-linked glycans	888:903	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	10	4	theme	method	1440:1445	arg1	validity					1414:1421	The validity	1410:1421	The validity of the developed method	1410:1445	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	8	5	dep	aiming	1109:1114	arg1	extract					1119:1125	extract	1119:1125	extract	1119:1125	This strategy is aiming to extract LLO precursor from microalgae.
30534192	6	6	theme	LLO	857:859	arg1	precursor					861:869	the LLO precursor and synthesis	853:883	precursor	861:869	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	12	7	theme	Phaeodactylaceae	2100:2115	arg1	classes					2135:2141	the Phaeodactylaceae and Chlorophyceae classes	2096:2141	the Phaeodactylaceae and Chlorophyceae classes	2096:2141	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	10	8	theme	structures	1500:1509	arg1	analysis					1468:1475	the analysis	1464:1475	the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1464:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	12	9	theme	Chlorophyceae	2121:2133	arg1	classes					2135:2141	the Phaeodactylaceae and Chlorophyceae classes	2096:2141	the Phaeodactylaceae and Chlorophyceae classes	2096:2141	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	10	10	theme	wild-type	1687:1695	arg1	cells					1697:1701	the wild-type cells	1683:1701	the wild-type cells	1683:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	2	11	theme	neo-synthesized	329:343	arg1	proteins					345:352	neo-synthesized proteins	329:352	neo-synthesized proteins	329:352	This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins.
30534192	1	12	theme	lipid-linked	236:247	arg1	LLO					267:269	LLO	267:269	LLO	267:269	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	1	12	theme	lipid-linked	236:247	arg1	oligosaccharides					249:264	lipid-linked oligosaccharides	236:264	a lipid-linked oligosaccharides (LLO) precursor	234:280	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	4	13	theme	complex	704:710	arg1	N-glycans					717:725	complex type N-glycans	704:725	complex type N-glycans	704:725	In this compartment, the N-linked glycans are further maturated in complex type N-glycans.
30534192	9	14	theme	green	1315:1319	arg1	reinhardtii					1347:1357	the green microalgae, Chlamydomonas reinhardtii	1311:1357	reinhardtii	1347:1357	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	0	15	from	analysis	71:78	arg1	microalgae					116:125	microalgae	116:125	microalgae	116:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	10	16	attach	released	1511:1518	arg2	structures					1500:1509	the oligosaccharide structures	1480:1509	the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1480:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	10	16	attach	released	1511:1518	arg1	LLO					1529:1531	the LLO	1525:1531	the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1525:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	7	17	theme	purification	1059:1070	arg1	steps					1072:1076	purification steps	1059:1076	purification steps	1059:1076	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	9	18	from	spectrometry	1263:1274	arg1	models					1283:1288	two models	1279:1288	two models of microalgae	1279:1302	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	11	19	theme	Glc2Man9GlcNAc2	1838:1852	arg1	structure					1854:1862	a Glc2Man9GlcNAc2 structure	1836:1862	a Glc2Man9GlcNAc2 structure	1836:1862	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	8	20	from	microalgae	1146:1155	arg1	extract					1119:1125	extract	1119:1125	extract	1119:1125	This strategy is aiming to extract LLO precursor from microalgae.
30534192	0	21	theme	lipid-linked	83:94	arg1	oligosaccharides					96:111	lipid-linked oligosaccharides	83:111	lipid-linked oligosaccharides in microalgae	83:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	13	22	theme	LLO	2226:2228	arg1	moiety					2246:2251	the LLO oligosaccharide moiety	2222:2251	the LLO oligosaccharide moiety	2222:2251	The method is fully adaptable for extracting and characterizing the LLO oligosaccharide moiety from microalgae belonging to other phyla.
30534192	7	23	theme	solvent	1027:1033	arg1	extractions					1035:1045	microsomal enrichment and solvent extractions	1001:1045	microsomal enrichment and solvent extractions	1001:1045	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	5	24	theme	eukaryotes	775:784	arg1	eukaryotes					775:784	eukaryotes	775:784	eukaryotes including higher plants	775:808	This process has been well studied in a lot of eukaryotes including higher plants.
30534192	5	24	theme	eukaryotes	775:784	arg1	plants					803:808	higher plants	796:808	higher plants	796:808	This process has been well studied in a lot of eukaryotes including higher plants.
30534192	5	24	theme	eukaryotes	775:784	arg1	lot					768:770	a lot	766:770	a lot of eukaryotes including higher plants	766:808	This process has been well studied in a lot of eukaryotes including higher plants.
30534192	12	25	theme	microalgae	2053:2062	arg1	LLO					2046:2048	LLO	2046:2048	LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	2046:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	7	26	theme	enrichment	1012:1021	arg1	extractions					1035:1045	microsomal enrichment and solvent extractions	1001:1045	microsomal enrichment and solvent extractions	1001:1045	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	5	27	theme	higher	796:801	arg1	plants					803:808	higher plants	796:808	higher plants	796:808	This process has been well studied in a lot of eukaryotes including higher plants.
30534192	9	28	attach	released	1191:1198	arg1	LLO					1219:1221	the extracted LLO	1205:1221	the extracted LLO	1205:1221	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	9	28	attach	released	1191:1198	arg2	moiety					1184:1189	the oligosaccharide moiety	1164:1189	the oligosaccharide moiety released from the extracted LLO	1164:1221	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	3	29	theme	N-linked	441:448	arg1	glycans					450:456	The N-linked glycans	437:456	The N-linked glycans bore by the glycoproteins	437:482	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	10	30	theme	mutants	1559:1565	arg1	LLO					1529:1531	the LLO	1525:1531	the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1525:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	3	31	gly	glycoproteins	549:561	arg1	glycoproteins					549:561	the glycoproteins	545:561	the glycoproteins	545:561	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	0	32	theme	User-friendly	0:12	arg1	extraction					14:23	User-friendly extraction	0:23	User-friendly extraction	0:23	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	7	33	theme	extraction	973:982	arg1	method					984:989	a user-friendly extraction method	957:989	a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps	957:1076	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	9	34	theme	oligosaccharide	1168:1182	arg1	moiety					1184:1189	the oligosaccharide moiety	1164:1189	the oligosaccharide moiety released from the extracted LLO	1164:1221	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	12	35	theme	tandem	1970:1975	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	1	36	theme	BACKGROUND	128:137	arg1	N-glycosylation					147:161	BACKGROUND Protein N-glycosylation	128:161	BACKGROUND Protein N-glycosylation	128:161	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	12	37	attach	released	2032:2039	arg1	LLO					2046:2048	LLO	2046:2048	LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	2046:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	12	37	attach	released	2032:2039	arg2	oligosaccharides					2015:2030	oligosaccharides	2015:2030	oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	2015:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	0	38	theme	tandem	40:45	arg1	spectrometry					52:63	multistage tandem mass spectrometry	29:63	multistage tandem mass spectrometry	29:63	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	12	39	theme	spectrometry	1982:1993	arg1	characterization					1995:2010	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization	1920:2010	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	4	40	link	N-linked	662:669	arg1	glycans					671:677	the N-linked glycans	658:677	the N-linked glycans	658:677	In this compartment, the N-linked glycans are further maturated in complex type N-glycans.
30534192	11	41	theme	diatom	1782:1787	arg1	LLO					1771:1773	the LLO	1767:1773	the LLO of the diatom P. tricornutum	1767:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	2	42	theme	oligosaccharyltransferase	380:404	arg1	action					366:371	the action	362:371	the action of the oligosaccharyltransferase giving birth to glycoproteins	362:434	This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins.
30534192	12	43	theme	non-radioactive	1930:1944	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	6	44	theme	glycans	897:903	arg1	synthesis					875:883	the LLO precursor and synthesis	853:883	synthesis	875:883	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	6	44	theme	glycans	897:903	arg1	precursor					861:869	the LLO precursor and synthesis	853:883	precursor	861:869	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	11	45	theme	first	1825:1829	arg1	time					1831:1834	the first time	1821:1834	the first time	1821:1834	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	7	46	theme	user-friendly	959:971	arg1	method					984:989	a user-friendly extraction method	957:989	a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps	957:1076	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	12	47	theme	reliable	1950:1957	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	3	48	theme	endoplasmic	572:582	arg1	reticulum					584:592	the endoplasmic reticulum	568:592	the endoplasmic reticulum	568:592	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	1	49	theme	precursor	272:280	arg1	synthesis					221:229	the synthesis	217:229	the synthesis of a lipid-linked oligosaccharides (LLO) precursor	217:280	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	9	50	theme	multistage	1240:1249	arg1	spectrometry					1263:1274	multistage tandem mass spectrometry	1240:1274	multistage tandem mass spectrometry in two models of microalgae	1240:1302	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	9	51	theme	mass	1258:1261	arg1	spectrometry					1263:1274	multistage tandem mass spectrometry	1240:1274	multistage tandem mass spectrometry in two models of microalgae	1240:1302	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	3	52	from	reticulum	584:592	arg1	entrance					602:609	its entrance	598:609	its entrance into the Golgi apparatus	598:634	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	3	52	from	reticulum	584:592	arg1	exit					537:540	the exit	533:540	the exit of the glycoproteins from the endoplasmic reticulum	533:592	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	2	53	theme	en	318:319	arg1	bloc					321:324	en bloc	318:324	en bloc on neo-synthesized proteins	318:352	This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins.
30534192	4	54	theme	type	712:715	arg1	N-glycans					717:725	complex type N-glycans	704:725	complex type N-glycans	704:725	In this compartment, the N-linked glycans are further maturated in complex type N-glycans.
30534192	6	55	from	available	908:916	arg1	microalgae					921:930	microalgae	921:930	microalgae	921:930	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	6	55	from	available	908:916	arg1	contrast					814:821	contrast	814:821	contrast	814:821	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	7	56	dep	METHODS	933:939	arg1	described					1081:1089	described	1081:1089	is described	1078:1089	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	10	57	theme	cells	1697:1701	arg1	one					1676:1678	the one	1672:1678	the one of the wild-type cells	1672:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	6	58	theme	little	824:829	arg1	information					831:841	little information	824:841	little information regarding the LLO precursor and synthesis of N-linked glycans	824:903	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	9	59	theme	microalgae	1293:1302	arg1	models					1283:1288	two models	1279:1288	two models of microalgae	1279:1302	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	0	60	from	oligosaccharides	96:111	arg1	microalgae					116:125	microalgae	116:125	microalgae	116:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	13	61	theme	other	2282:2286	arg1	phyla					2288:2292	other phyla	2282:2292	other phyla	2282:2292	The method is fully adaptable for extracting and characterizing the LLO oligosaccharide moiety from microalgae belonging to other phyla.
30534192	1	62	theme	oligosaccharides	249:264	arg1	precursor					272:280	a lipid-linked oligosaccharides (LLO) precursor	234:280	a lipid-linked oligosaccharides (LLO) precursor	234:280	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	6	63	from	microalgae	921:930	arg1	available					908:916	available	908:916	available	908:916	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	9	64	theme	microalgae	1321:1330	arg1	reinhardtii					1347:1357	the green microalgae, Chlamydomonas reinhardtii	1311:1357	reinhardtii	1347:1357	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	1	65	theme	Protein	139:145	arg1	N-glycosylation					147:161	BACKGROUND Protein N-glycosylation	128:161	BACKGROUND Protein N-glycosylation	128:161	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	10	66	theme	oligosaccharide	1484:1498	arg1	structures					1500:1509	the oligosaccharide structures	1480:1509	the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1480:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	10	67	dep	RESULTS	1402:1408	arg1	confirmed					1451:1459	confirmed	1451:1459	was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1447:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	0	68	from	microalgae	116:125	arg1	analysis					71:78	analysis	71:78	analysis of lipid-linked oligosaccharides in microalgae	71:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	3	69	link	N-linked	441:448	arg1	glycans					450:456	The N-linked glycans	437:456	The N-linked glycans bore by the glycoproteins	437:482	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	2	70	from	bloc	321:324	arg1	proteins					345:352	neo-synthesized proteins	329:352	neo-synthesized proteins	329:352	This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins.
30534192	0	71	theme	oligosaccharides	96:111	arg1	analysis					71:78	analysis	71:78	analysis of lipid-linked oligosaccharides in microalgae	71:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	3	72	gly	glycoproteins	470:482	arg1	glycoproteins					470:482	the glycoproteins	466:482	the glycoproteins	466:482	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	1	73	theme	endoplasmic	187:197	arg1	reticulum					199:207	the endoplasmic reticulum	183:207	the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor	183:280	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	12	74	dep	CONCLUSION	1865:1874	arg1	allows					1913:1918	allows	1913:1918	allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1913:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	13	75	theme	oligosaccharide	2230:2244	arg1	moiety					2246:2251	the LLO oligosaccharide moiety	2222:2251	the LLO oligosaccharide moiety	2222:2251	The method is fully adaptable for extracting and characterizing the LLO oligosaccharide moiety from microalgae belonging to other phyla.
30534192	3	76	theme	Golgi	620:624	arg1	apparatus					626:634	the Golgi apparatus	616:634	the Golgi apparatus	616:634	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	1	77	link	lipid-linked	236:247	arg1	LLO					267:269	LLO	267:269	LLO	267:269	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	1	77	link	lipid-linked	236:247	arg1	oligosaccharides					249:264	lipid-linked oligosaccharides	236:264	a lipid-linked oligosaccharides (LLO) precursor	234:280	BACKGROUND Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor.
30534192	8	78	theme	LLO	1127:1129	arg1	precursor					1131:1139	LLO precursor	1127:1139	LLO precursor	1127:1139	This strategy is aiming to extract LLO precursor from microalgae.
30534192	7	79	theme	microsomal	1001:1010	arg1	extractions					1035:1045	microsomal enrichment and solvent extractions	1001:1045	microsomal enrichment and solvent extractions	1001:1045	METHODS In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described.
30534192	10	80	theme	xylosyltransferase	1540:1557	arg1	mutants					1559:1565	two xylosyltransferase mutants	1536:1565	two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1536:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	12	81	theme	oligosaccharides	2015:2030	arg1	characterization					1995:2010	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization	1920:2010	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	0	82	theme	multistage	29:38	arg1	spectrometry					52:63	multistage tandem mass spectrometry	29:63	multistage tandem mass spectrometry	29:63	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	12	83	theme	mass	1977:1980	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	2	84	gly	glycoproteins	422:434	arg1	glycoproteins					422:434	glycoproteins	422:434	glycoproteins	422:434	This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins.
30534192	11	85	theme	P.	1789:1790	arg1	diatom					1782:1787	the diatom P. tricornutum	1778:1802	the diatom P. tricornutum	1778:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	0	86	theme	mass	47:50	arg1	spectrometry					52:63	multistage tandem mass spectrometry	29:63	multistage tandem mass spectrometry	29:63	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	10	87	theme	reinhardtii	1573:1583	arg1	mutants					1559:1565	two xylosyltransferase mutants	1536:1565	two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1536:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	10	88	theme	developed	1430:1438	arg1	method					1440:1445	the developed method	1426:1445	the developed method	1426:1445	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	0	89	link	lipid-linked	83:94	arg1	oligosaccharides					96:111	lipid-linked oligosaccharides	83:111	lipid-linked oligosaccharides in microalgae	83:125	User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae.
30534192	9	90	theme	extracted	1209:1217	arg1	LLO					1219:1221	the extracted LLO	1205:1221	the extracted LLO	1205:1221	Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.
30534192	3	91	theme	glycoproteins	549:561	arg1	entrance					602:609	its entrance	598:609	its entrance into the Golgi apparatus	598:634	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	3	91	theme	glycoproteins	549:561	arg1	exit					537:540	the exit	533:540	the exit of the glycoproteins from the endoplasmic reticulum	533:592	The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus.
30534192	12	92	theme	multistage	1959:1968	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	6	93	theme	N-linked	888:895	arg1	glycans					897:903	N-linked glycans	888:903	N-linked glycans	888:903	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	12	94	theme	fast	1924:1927	arg1	spectrometry					1982:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry	1920:1993	the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively	1920:2155	CONCLUSION The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively.
30534192	6	95	from	contrast	814:821	arg1	available					908:916	available	908:916	available	908:916	In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.
30534192	4	96	theme	N-linked	662:669	arg1	glycans					671:677	the N-linked glycans	658:677	the N-linked glycans	658:677	In this compartment, the N-linked glycans are further maturated in complex type N-glycans.
30534192	10	97	theme	green	1606:1610	arg1	microalga					1612:1620	this green microalga	1601:1620	this green microalga	1601:1620	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	11	98	dep	diatom	1782:1787	arg1	tricornutum					1792:1802	the diatom P. tricornutum	1778:1802	the diatom P. tricornutum	1778:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	10	99	theme	identical	1659:1667	arg1	Glc3Man5GlcNAc2					1643:1657	a linear Glc3Man5GlcNAc2	1634:1657	a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells	1634:1701	RESULTS The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells.
30534192	11	100	theme	oligosaccharide	1737:1751	arg1	analysis					1721:1728	the analysis	1717:1728	the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum	1717:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	11	101	attach	released	1753:1760	arg1	LLO					1771:1773	the LLO	1767:1773	the LLO of the diatom P. tricornutum	1767:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
30534192	11	101	attach	released	1753:1760	arg2	oligosaccharide					1737:1751	the oligosaccharide	1733:1751	the oligosaccharide released from the LLO of the diatom P. tricornutum	1733:1802	In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.
29410370	0	0	theme	eco-friendly	77:88	arg1	bionanocomposites					113:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	5	1	theme	water	718:722	arg1	solubility					724:733	the water solubility	714:733	the water solubility	714:733	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	1	2	theme	soluble	174:180	arg1	SSPS					206:209	SSPS	206:209	SSPS	206:209	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	1	2	theme	soluble	174:180	arg1	polysaccharide					190:203	soluble soybean polysaccharide	174:203	soluble soybean polysaccharide (SSPS)	174:210	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	11	3	theme	antimicrobial	1464:1476	arg1	composite					1478:1486	a new antimicrobial composite	1458:1486	a new antimicrobial composite which is potentially useful to prevent and treat infections	1458:1546	The current research clearly introduces a new antimicrobial composite which is potentially useful to prevent and treat infections.
29410370	11	3	theme	antimicrobial	1464:1476	arg1	useful					1509:1514	useful	1509:1514	useful	1509:1514	The current research clearly introduces a new antimicrobial composite which is potentially useful to prevent and treat infections.
29410370	6	4	theme	5wt	947:949	arg1	TiO2					952:955	5wt% TiO2	947:955	5wt% TiO2	947:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	10	5	theme	promising	1375:1383	arg1	range					1385:1389	a promising range	1373:1389	a promising range of antimicrobial activity	1373:1415	SSPS-based nanocomposite films demonstrated a promising range of antimicrobial activity.
29410370	1	6	theme	soybean	182:188	arg1	SSPS					206:209	SSPS	206:209	SSPS	206:209	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	1	6	theme	soybean	182:188	arg1	polysaccharide					190:203	soluble soybean polysaccharide	174:203	soluble soybean polysaccharide (SSPS)	174:210	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	1	7	theme	various	282:288	arg1	concentrations					290:303	various concentrations	282:303	various concentrations of TiO2 nanoparticles	282:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	0	8	theme	nanoparticle	100:111	arg1	bionanocomposites					113:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	2	9	theme	13C	328:330	arg1	NMR					332:334	13C NMR	328:334	13C NMR	328:334	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	4	10	theme	SSPS	628:631	arg1	lower					644:648	lower	644:648	lower	644:648	The intrinsic viscosity of SSPS (0.33) was lower than most of hydrocolloids.
29410370	4	10	theme	SSPS	628:631	arg1	viscosity					615:623	The intrinsic viscosity	601:623	The intrinsic viscosity of SSPS (0.33)	601:638	The intrinsic viscosity of SSPS (0.33) was lower than most of hydrocolloids.
29410370	0	11	theme	SSPS/TiO2	90:98	arg1	bionanocomposites					113:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	77:129	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	8	12	dep	increased	1134:1142	arg1	reached					1185:1191	reached	1185:1191	reached 15wt%	1185:1197	There were no significant agglomerates when the TiO2 concentrations were increased to 5wt%; however, when the concentration reached 15wt%, agglomerations were observed.
29410370	8	12	dep	increased	1134:1142	arg1	observed					1220:1227	observed	1220:1227	were observed	1215:1227	There were no significant agglomerates when the TiO2 concentrations were increased to 5wt%; however, when the concentration reached 15wt%, agglomerations were observed.
29410370	6	13	theme	SSPS	923:926	arg1	nanocomposite					928:940	the SSPS nanocomposite	919:940	the SSPS nanocomposite with 5wt% TiO2	919:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	3	14	theme	SSPS	526:529	arg1	Mn					488:489	Mn	488:489	Mn	488:489	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	14	theme	SSPS	526:529	arg1	weight					480:485	number average molecular weight	455:485	number average molecular weight (Mn)	455:490	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	14	theme	SSPS	526:529	arg1	weight					447:452	Weight average molecular weight	422:452	Weight average molecular weight	422:452	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	14	theme	SSPS	526:529	arg1	index					511:515	polydispersity index	496:515	polydispersity index (PDI)	496:521	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	14	theme	SSPS	526:529	arg1	PDI					518:520	PDI	518:520	PDI	518:520	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	5	15	theme	films	816:820	arg1	content					745:751	moisture content	736:751	moisture content	736:751	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	15	theme	films	816:820	arg1	permeability					769:780	water-vapor permeability	757:780	water-vapor permeability (WVP) of SSPS-based nanocomposite films	757:820	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	15	theme	films	816:820	arg1	WVP					783:785	WVP	783:785	WVP	783:785	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	15	theme	films	816:820	arg1	solubility					724:733	the water solubility	714:733	the water solubility	714:733	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	16	theme	TiO2	694:697	arg1	concentration					699:711	TiO2 concentration	694:711	TiO2 concentration	694:711	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	7	17	theme	melting	1015:1021	arg1	temperature					1023:1033	the melting temperature	1011:1033	the melting temperature	1011:1033	With increasing TiO2 concentrations from 5 to 15wt%, the melting temperature declined from 24 to 19°C.
29410370	3	18	theme	molecular	470:478	arg1	Mn					488:489	Mn	488:489	Mn	488:489	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	18	theme	molecular	470:478	arg1	weight					480:485	number average molecular weight	455:485	number average molecular weight (Mn)	455:490	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	1	19	theme	polysaccharide	190:203	arg1	characterization					154:169	characterization	154:169	characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles	154:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	0	20	theme	bionanocomposites	113:129	arg1	Characterization					0:15	Characterization	0:15	Characterization of soluble soybean (SSPS) polysaccharide	0:56	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	0	20	theme	bionanocomposites	113:129	arg1	development					62:72	development	62:72	development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	62:129	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	10	21	theme	activity	1408:1415	arg1	range					1385:1389	a promising range	1373:1389	a promising range of antimicrobial activity	1373:1415	SSPS-based nanocomposite films demonstrated a promising range of antimicrobial activity.
29410370	2	22	theme	-α	409:410	arg1	Rhap					412:415	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	5	23	theme	water-vapor	757:767	arg1	permeability					769:780	water-vapor permeability	757:780	water-vapor permeability (WVP) of SSPS-based nanocomposite films	757:820	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	23	theme	water-vapor	757:767	arg1	WVP					783:785	WVP	783:785	WVP	783:785	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	10	24	theme	antimicrobial	1394:1406	arg1	activity					1408:1415	antimicrobial activity	1394:1415	antimicrobial activity	1394:1415	SSPS-based nanocomposite films demonstrated a promising range of antimicrobial activity.
29410370	10	25	theme	nanocomposite	1340:1352	arg1	films					1354:1358	SSPS-based nanocomposite films	1329:1358	SSPS-based nanocomposite films	1329:1358	SSPS-based nanocomposite films demonstrated a promising range of antimicrobial activity.
29410370	3	26	theme	Weight	422:427	arg1	weight					447:452	Weight average molecular weight	422:452	Weight average molecular weight	422:452	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	1	27	theme	TiO2	308:311	arg1	nanoparticles					313:325	TiO2 nanoparticles	308:325	TiO2 nanoparticles	308:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	10	28	theme	SSPS-based	1329:1338	arg1	films					1354:1358	SSPS-based nanocomposite films	1329:1358	SSPS-based nanocomposite films	1329:1358	SSPS-based nanocomposite films demonstrated a promising range of antimicrobial activity.
29410370	7	29	dep	19°C	1055:1058	arg1	to					1052:1053	to	1052:1053	to	1052:1053	With increasing TiO2 concentrations from 5 to 15wt%, the melting temperature declined from 24 to 19°C.
29410370	3	30	theme	average	429:435	arg1	weight					447:452	Weight average molecular weight	422:452	Weight average molecular weight	422:452	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	0	31	theme	soybean	28:34	arg1	polysaccharide					43:56	soluble soybean (SSPS) polysaccharide	20:56	soluble soybean (SSPS) polysaccharide	20:56	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	1	32	theme	nanoparticles	313:325	arg1	concentrations					290:303	various concentrations	282:303	various concentrations of TiO2 nanoparticles	282:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	3	33	theme	average	462:468	arg1	Mn					488:489	Mn	488:489	Mn	488:489	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	33	theme	average	462:468	arg1	weight					480:485	number average molecular weight	455:485	number average molecular weight (Mn)	455:490	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	0	34	theme	soluble	20:26	arg1	polysaccharide					43:56	soluble soybean (SSPS) polysaccharide	20:56	soluble soybean (SSPS) polysaccharide	20:56	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	6	35	theme	132°C	909:913	arg1	maximum					898:904	a maximum	896:904	a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2	896:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	6	36	theme	TiO2	833:836	arg1	addition					838:845	TiO2 addition	833:845	TiO2 addition	833:845	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	2	37	theme	[1→4	390:393	arg1	Rhap					412:415	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	1	38	theme	development	216:226	arg1	characterization					154:169	characterization	154:169	characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles	154:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	0	39	theme	SSPS	37:40	arg1	polysaccharide					43:56	soluble soybean (SSPS) polysaccharide	20:56	soluble soybean (SSPS) polysaccharide	20:56	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	5	40	theme	SSPS-based	791:800	arg1	films					816:820	SSPS-based nanocomposite films	791:820	SSPS-based nanocomposite films	791:820	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	3	41	theme	molecular	437:445	arg1	weight					447:452	Weight average molecular weight	422:452	Weight average molecular weight	422:452	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	9	42	theme	tensile	1267:1273	arg1	strength					1275:1282	tensile strength	1267:1282	tensile strength	1267:1282	With addition of TiO2 nanoparticles, tensile strength increased but elongation at break decreased.
29410370	5	43	theme	moisture	736:743	arg1	content					745:751	moisture content	736:751	moisture content	736:751	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	5	44	theme	nanocomposite	802:814	arg1	films					816:820	SSPS-based nanocomposite films	791:820	SSPS-based nanocomposite films	791:820	With increasing TiO2 concentration, the water solubility, moisture content and water-vapor permeability (WVP) of SSPS-based nanocomposite films decreased.
29410370	3	45	theme	number	455:460	arg1	Mn					488:489	Mn	488:489	Mn	488:489	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	45	theme	number	455:460	arg1	weight					480:485	number average molecular weight	455:485	number average molecular weight (Mn)	455:490	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	0	46	theme	polysaccharide	43:56	arg1	Characterization					0:15	Characterization	0:15	Characterization of soluble soybean (SSPS) polysaccharide	0:56	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	0	46	theme	polysaccharide	43:56	arg1	development					62:72	development	62:72	development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites	62:129	Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.
29410370	8	47	theme	significant	1075:1085	arg1	agglomerates					1087:1098	no significant agglomerates	1072:1098	no significant agglomerates	1072:1098	There were no significant agglomerates when the TiO2 concentrations were increased to 5wt%; however, when the concentration reached 15wt%, agglomerations were observed.
29410370	9	48	from	break	1312:1316	arg1	elongation					1298:1307	elongation	1298:1307	elongation at break	1298:1316	With addition of TiO2 nanoparticles, tensile strength increased but elongation at break decreased.
29410370	9	49	theme	TiO2	1247:1250	arg1	nanoparticles					1252:1264	TiO2 nanoparticles	1247:1264	TiO2 nanoparticles	1247:1264	With addition of TiO2 nanoparticles, tensile strength increased but elongation at break decreased.
29410370	2	50	theme	rhamnogalacturonan	371:388	arg1	Rhap					412:415	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	8	51	theme	5wt	1147:1149	arg1	%					1150:1150	5wt%	1147:1150	5wt%	1147:1150	There were no significant agglomerates when the TiO2 concentrations were increased to 5wt%; however, when the concentration reached 15wt%, agglomerations were observed.
29410370	3	52	theme	polydispersity	496:509	arg1	PDI					518:520	PDI	518:520	PDI	518:520	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	3	52	theme	polydispersity	496:509	arg1	index					511:515	polydispersity index	496:515	polydispersity index (PDI)	496:521	Weight average molecular weight, number average molecular weight (Mn) and polydispersity index (PDI) of SSPS were found to be 2.54×106g/mol, 5.54×106g/mol, and 4.5, respectively.
29410370	8	53	theme	TiO2	1109:1112	arg1	concentrations					1114:1127	the TiO2 concentrations	1105:1127	the TiO2 concentrations	1105:1127	There were no significant agglomerates when the TiO2 concentrations were increased to 5wt%; however, when the concentration reached 15wt%, agglomerations were observed.
29410370	6	54	with	nanocomposite	928:940	arg1	TiO2					952:955	5wt% TiO2	947:955	5wt% TiO2	947:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	9	55	theme	nanoparticles	1252:1264	arg1	addition					1235:1242	addition	1235:1242	addition of TiO2 nanoparticles	1235:1264	With addition of TiO2 nanoparticles, tensile strength increased but elongation at break decreased.
29410370	2	56	theme	SSPS	363:366	arg1	backbone					351:358	backbone	351:358	backbone of SSPS	351:366	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	1	57	theme	biodegradable	233:245	arg1	nanocomposites					252:265	a biodegradable SSPS nanocomposites	231:265	a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles	231:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	7	58	theme	TiO2	974:977	arg1	concentrations					979:992	TiO2 concentrations	974:992	TiO2 concentrations	974:992	With increasing TiO2 concentrations from 5 to 15wt%, the melting temperature declined from 24 to 19°C.
29410370	7	59	dep	15wt	1004:1007	arg1	to					1001:1002	to	1001:1002	to	1001:1002	With increasing TiO2 concentrations from 5 to 15wt%, the melting temperature declined from 24 to 19°C.
29410370	1	60	theme	SSPS	247:250	arg1	nanocomposites					252:265	a biodegradable SSPS nanocomposites	231:265	a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles	231:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	4	61	theme	intrinsic	605:613	arg1	lower					644:648	lower	644:648	lower	644:648	The intrinsic viscosity of SSPS (0.33) was lower than most of hydrocolloids.
29410370	4	61	theme	intrinsic	605:613	arg1	viscosity					615:623	The intrinsic viscosity	601:623	The intrinsic viscosity of SSPS (0.33)	601:638	The intrinsic viscosity of SSPS (0.33) was lower than most of hydrocolloids.
29410370	11	62	theme	current	1422:1428	arg1	research					1430:1437	The current research	1418:1437	The current research	1418:1437	The current research clearly introduces a new antimicrobial composite which is potentially useful to prevent and treat infections.
29410370	1	63	theme	nanocomposites	252:265	arg1	SSPS					206:209	SSPS	206:209	SSPS	206:209	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	1	63	theme	nanocomposites	252:265	arg1	development					216:226	development	216:226	development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles	216:325	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	1	63	theme	nanocomposites	252:265	arg1	polysaccharide					190:203	soluble soybean polysaccharide	174:203	soluble soybean polysaccharide (SSPS)	174:210	This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles.
29410370	6	64	from	increase	857:864	arg1	temperature					881:891	the melting temperature	869:891	the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2	869:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	6	65	theme	melting	873:879	arg1	temperature					881:891	the melting temperature	869:891	the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2	869:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29410370	2	66	theme	-α-GalAp-	395:403	arg1	Rhap					412:415	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]	371:419	13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→].
29410370	11	67	theme	new	1460:1462	arg1	composite					1478:1486	a new antimicrobial composite	1458:1486	a new antimicrobial composite which is potentially useful to prevent and treat infections	1458:1546	The current research clearly introduces a new antimicrobial composite which is potentially useful to prevent and treat infections.
29410370	11	67	theme	new	1460:1462	arg1	useful					1509:1514	useful	1509:1514	useful	1509:1514	The current research clearly introduces a new antimicrobial composite which is potentially useful to prevent and treat infections.
29410370	6	68	theme	%	950:950	arg1	TiO2					952:955	5wt% TiO2	947:955	5wt% TiO2	947:955	TiO2 addition led to an increase in the melting temperature to a maximum of 132°C for the SSPS nanocomposite with 5wt% TiO2.
29442717	0	0	theme	L-lactic	72:79	arg1	D					70:70	D	70:70	D	70:70	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	0	0	theme	L-lactic	72:79	arg1	acid					81:84	L-lactic acid	72:84	L-lactic acid	72:84	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	7	1	theme	microspheres	1094:1105	arg1	preparation					1054:1064	the preparation	1050:1064	the preparation of β-TCP/SA/PDLLA composite microspheres	1050:1105	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	4	2	theme	L-lactic	627:634	arg1	D					625:625	D	625:625	D	625:625	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	2	theme	L-lactic	627:634	arg1	acid					636:639	L-lactic acid	627:639	L-lactic acid	627:639	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	1	3	theme	Bone	142:145	arg1	microspheres					154:165	Bone repair microspheres	142:165	Bone repair microspheres	142:165	Bone repair microspheres have been widely studied due to their convenience during clinical operations.
29442717	5	4	dep	-substituted	817:828	arg1	CO3					813:815	CO3	813:815	CO3	813:815	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	4	dep	-substituted	817:828	arg1	Na					809:810	Na	809:810	Na	809:810	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	1	5	theme	repair	147:152	arg1	microspheres					154:165	Bone repair microspheres	142:165	Bone repair microspheres	142:165	Bone repair microspheres have been widely studied due to their convenience during clinical operations.
29442717	10	6	theme	β-TCP/SA/PDLLA	1426:1439	arg1	material					1499:1506	a promising graft material	1481:1506	a promising graft material particularly for bone repair	1481:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	6	theme	β-TCP/SA/PDLLA	1426:1439	arg1	microspheres					1451:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	7	7	theme	β-TCP/SA/PDLLA	1069:1082	arg1	microspheres					1094:1105	β-TCP/SA/PDLLA composite microspheres	1069:1105	β-TCP/SA/PDLLA composite microspheres	1069:1105	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	2	8	theme	phosphate/sodium	276:291	arg1	β-TCP/SA/PDLLA					325:338	β-TCP/SA/PDLLA	325:338	β-TCP/SA/PDLLA	325:338	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	8	theme	phosphate/sodium	276:291	arg1	alginate/poly					293:305	beta tricalcium phosphate/sodium alginate/poly	260:305	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	5	9	theme	-substituted	817:828	arg1	products					778:785	the final products	768:785	the final products	768:785	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	9	theme	-substituted	817:828	arg1	HA					830:831	pure HA but (Na, CO3)-substituted HA	796:831	pure HA but (Na, CO3)-substituted HA	796:831	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	7	10	theme	composite	1084:1092	arg1	microspheres					1094:1105	β-TCP/SA/PDLLA composite microspheres	1069:1105	β-TCP/SA/PDLLA composite microspheres	1069:1105	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	10	11	theme	graft	1493:1497	arg1	microspheres					1451:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	11	theme	graft	1493:1497	arg1	material					1499:1506	a promising graft material	1481:1506	a promising graft material particularly for bone repair	1481:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	2	12	theme	tricalcium	265:274	arg1	β-TCP/SA/PDLLA					325:338	β-TCP/SA/PDLLA	325:338	β-TCP/SA/PDLLA	325:338	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	12	theme	tricalcium	265:274	arg1	alginate/poly					293:305	beta tricalcium phosphate/sodium alginate/poly	260:305	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	3	13	theme	simulated	483:491	arg1	SBF					506:508	SBF	506:508	SBF	506:508	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	3	13	theme	simulated	483:491	arg1	fluids					498:503	simulated body fluids	483:503	simulated body fluids (SBF) for 7 days	483:520	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	0	14	theme	Microsphere	97:107	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug	0:120	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	5	15	theme	HA	801:802	arg1	products					778:785	the final products	768:785	the final products	768:785	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	15	theme	HA	801:802	arg1	HA					830:831	pure HA but (Na, CO3)-substituted HA	796:831	pure HA but (Na, CO3)-substituted HA	796:831	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	16	theme	bicarbonate	736:746	arg1	ions					748:751	bicarbonate ions	736:751	bicarbonate ions	736:751	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	2	17	theme	beta	260:263	arg1	β-TCP/SA/PDLLA					325:338	β-TCP/SA/PDLLA	325:338	β-TCP/SA/PDLLA	325:338	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	17	theme	beta	260:263	arg1	alginate/poly					293:305	beta tricalcium phosphate/sodium alginate/poly	260:305	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	0	18	theme	Composite	87:95	arg1	Microsphere					97:107	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	5	19	theme	pure	796:799	arg1	products					778:785	the final products	768:785	the final products	768:785	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	19	theme	pure	796:799	arg1	HA					830:831	pure HA but (Na, CO3)-substituted HA	796:831	pure HA but (Na, CO3)-substituted HA	796:831	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	7	20	theme	embedding	1193:1201	arg1	ratio					1203:1207	a final embedding ratio	1185:1207	a final embedding ratio of 26.18%	1185:1217	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	3	21	theme	β-TCP/SA/PDLLA	430:443	arg1	microspheres					455:466	β-TCP/SA/PDLLA composite microspheres	430:466	β-TCP/SA/PDLLA composite microspheres	430:466	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	8	22	theme	composite	1252:1260	arg1	microspheres					1262:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	10	23	theme	bone	1525:1528	arg1	repair					1530:1535	bone repair	1525:1535	bone repair	1525:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	24	theme	promising	1483:1491	arg1	microspheres					1451:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	24	theme	promising	1483:1491	arg1	material					1499:1506	a promising graft material	1481:1506	a promising graft material particularly for bone repair	1481:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	3	25	theme	X-ray	540:544	arg1	XRD					562:564	XRD	562:564	XRD	562:564	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	3	25	theme	X-ray	540:544	arg1	diffractometer					546:559	an X-ray diffractometer	537:559	an X-ray diffractometer (XRD)	537:565	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	5	26	theme	final	772:776	arg1	products					778:785	the final products	768:785	the final products	768:785	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	5	26	theme	final	772:776	arg1	HA					830:831	pure HA but (Na, CO3)-substituted HA	796:831	pure HA but (Na, CO3)-substituted HA	796:831	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	0	27	theme	Novel	17:21	arg1	Alginate/Poly					56:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly	15:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	8	28	theme	vancomycin	1349:1358	arg1	release					1338:1344	the release	1334:1344	the release of vancomycin (92.8 wt	1334:1367	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	8	28	theme	vancomycin	1349:1358	arg1	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	7	29	theme	vancomycin	1141:1150	arg1	incorporation					1124:1136	the easy incorporation	1115:1136	the easy incorporation of vancomycin into the microspheres	1115:1172	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	4	30	theme	limiting	658:665	arg1	alginate					602:609	sodium alginate	595:609	sodium alginate (SA)	595:614	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	30	theme	limiting	658:665	arg1	factors					667:673	limiting factors	658:673	limiting factors	658:673	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	30	theme	limiting	658:665	arg1	poly					620:623	poly	620:623	poly(D,L-lactic acid) (PDLLA)	620:648	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	8	31	theme	sustained	1295:1303	arg1	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	4	32	theme	sodium	595:600	arg1	alginate					602:609	sodium alginate	595:609	sodium alginate (SA)	595:614	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	32	theme	sodium	595:600	arg1	factors					667:673	limiting factors	658:673	limiting factors	658:673	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	32	theme	sodium	595:600	arg1	SA					612:613	SA	612:613	SA	612:613	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	32	theme	sodium	595:600	arg1	poly					620:623	poly	620:623	poly(D,L-lactic acid) (PDLLA)	620:648	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	7	33	theme	calcination	1031:1041	arg1	absence					1020:1026	the absence	1016:1026	the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres	1016:1105	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	2	34	theme	alginate/poly	293:305	arg1	microspheres					351:362	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	35	theme	droplet	408:414	arg1	method					416:421	the liquid droplet method	397:421	the liquid droplet method	397:421	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	0	36	theme	Tricalcium	28:37	arg1	Alginate/Poly					56:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly	15:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	3	37	theme	composite	445:453	arg1	microspheres					455:466	β-TCP/SA/PDLLA composite microspheres	430:466	β-TCP/SA/PDLLA composite microspheres	430:466	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	0	38	dep	Alginate/Poly	56:68	arg1	D					70:70	D	70:70	D	70:70	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	0	38	dep	Alginate/Poly	56:68	arg1	acid					81:84	L-lactic acid	72:84	L-lactic acid	72:84	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	2	39	theme	liquid	401:406	arg1	method					416:421	the liquid droplet method	397:421	the liquid droplet method	397:421	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	0	40	theme	Beta	23:26	arg1	Alginate/Poly					56:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly	15:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	0	41	theme	Drug	117:120	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug	0:120	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	5	42	theme	sodium	720:725	arg1	ions					727:730	sodium ions	720:730	sodium ions	720:730	Since sodium ions and bicarbonate ions were abundant, the final products were not pure HA but (Na, CO3)-substituted HA.
29442717	6	43	theme	microspheres	896:907	arg1	structure					858:866	the structure	854:866	the structure of β-TCP/SA/PDLLA composite microspheres	854:907	When soaked in SBF, the structure of β-TCP/SA/PDLLA composite microspheres remained stable for at least 14 days suggesting that their anti-washout ability was suitable.
29442717	0	44	theme	Alginate/Poly	56:68	arg1	Microsphere					97:107	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	8	45	contain	possessed	1275:1283	arg2	release					1338:1344	the release	1334:1344	the release of vancomycin (92.8 wt	1334:1367	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	8	45	contain	possessed	1275:1283	arg1	microspheres					1262:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	8	45	contain	possessed	1275:1283	arg2	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	8	46	theme	release	1310:1316	arg1	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	8	47	theme	β-TCP/SA/PDLLA	1237:1250	arg1	microspheres					1262:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	the β-TCP/SA/PDLLA composite microspheres	1233:1273	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	3	48	theme	body	493:496	arg1	SBF					506:508	SBF	506:508	SBF	506:508	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	3	48	theme	body	493:496	arg1	fluids					498:503	simulated body fluids	483:503	simulated body fluids (SBF) for 7 days	483:520	Then, β-TCP/SA/PDLLA composite microspheres were soaked in simulated body fluids (SBF) for 7 days, and tested in an X-ray diffractometer (XRD).
29442717	0	49	theme	Phosphate/Sodium	39:54	arg1	Alginate/Poly					56:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly	15:68	a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere	15:107	Fabrication of a Novel Beta Tricalcium Phosphate/Sodium Alginate/Poly(D,L-lactic acid) Composite Microsphere and Its Drug Releasing Property.
29442717	10	50	used	used	1473:1476	arg2	material					1499:1506	a promising graft material	1481:1506	a promising graft material particularly for bone repair	1481:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	50	used	used	1473:1476	arg2	microspheres					1451:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	8	51	theme	drug	1305:1308	arg1	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	2	52	dep	alginate/poly	293:305	arg1	D					307:307	D	307:307	D	307:307	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	52	dep	alginate/poly	293:305	arg1	acid					318:321	L-lactic acid	309:321	L-lactic acid	309:321	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	8	53	dep	release	1338:1344	arg1	wt					1366:1367	92.8 wt	1361:1367	the release of vancomycin (92.8 wt	1334:1367	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	4	54	dep	poly	620:623	arg1	D					625:625	D	625:625	D	625:625	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	54	dep	poly	620:623	arg1	acid					636:639	L-lactic acid	627:639	L-lactic acid	627:639	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	4	55	theme	β-TCP	701:705	arg1	transformation					683:696	the transformation	679:696	the transformation of β-TCP to HA	679:711	The results indicated that sodium alginate (SA) and poly(D,L-lactic acid) (PDLLA) are not limiting factors for the transformation of β-TCP to HA.
29442717	8	56	theme	excellent	1285:1293	arg1	capability					1318:1327	excellent sustained drug release capability	1285:1327	excellent sustained drug release capability	1285:1327	Furthermore, the β-TCP/SA/PDLLA composite microspheres possessed excellent sustained drug release capability, and the release of vancomycin (92.8 wt.
29442717	6	57	theme	composite	886:894	arg1	microspheres					896:907	β-TCP/SA/PDLLA composite microspheres	871:907	β-TCP/SA/PDLLA composite microspheres	871:907	When soaked in SBF, the structure of β-TCP/SA/PDLLA composite microspheres remained stable for at least 14 days suggesting that their anti-washout ability was suitable.
29442717	2	58	theme	L-lactic	309:316	arg1	D					307:307	D	307:307	D	307:307	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	2	58	theme	L-lactic	309:316	arg1	acid					318:321	L-lactic acid	309:321	L-lactic acid	309:321	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
29442717	6	59	theme	β-TCP/SA/PDLLA	871:884	arg1	microspheres					896:907	β-TCP/SA/PDLLA composite microspheres	871:907	β-TCP/SA/PDLLA composite microspheres	871:907	When soaked in SBF, the structure of β-TCP/SA/PDLLA composite microspheres remained stable for at least 14 days suggesting that their anti-washout ability was suitable.
29442717	7	60	theme	final	1187:1191	arg1	ratio					1203:1207	a final embedding ratio	1185:1207	a final embedding ratio of 26.18%	1185:1217	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	6	61	theme	anti-washout	968:979	arg1	ability					981:987	their anti-washout ability	962:987	their anti-washout ability	962:987	When soaked in SBF, the structure of β-TCP/SA/PDLLA composite microspheres remained stable for at least 14 days suggesting that their anti-washout ability was suitable.
29442717	7	62	theme	%	1217:1217	arg1	ratio					1203:1207	a final embedding ratio	1185:1207	a final embedding ratio of 26.18%	1185:1217	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	1	63	theme	clinical	224:231	arg1	operations					233:242	clinical operations	224:242	clinical operations	224:242	Bone repair microspheres have been widely studied due to their convenience during clinical operations.
29442717	7	64	theme	easy	1119:1122	arg1	incorporation					1124:1136	the easy incorporation	1115:1136	the easy incorporation of vancomycin into the microspheres	1115:1172	Furthermore, the absence of calcination during the preparation of β-TCP/SA/PDLLA composite microspheres enabled the easy incorporation of vancomycin into the microspheres in situ at a final embedding ratio of 26.18%.
29442717	10	65	theme	composite	1441:1449	arg1	material					1499:1506	a promising graft material	1481:1506	a promising graft material particularly for bone repair	1481:1535	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	10	65	theme	composite	1441:1449	arg1	microspheres					1451:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	the β-TCP/SA/PDLLA composite microspheres	1422:1462	Our results suggest that the β-TCP/SA/PDLLA composite microspheres could be used as a promising graft material particularly for bone repair.
29442717	2	66	theme	composite	341:349	arg1	microspheres					351:362	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres	260:362	In this study, beta tricalcium phosphate/sodium alginate/poly(D,L-lactic acid) (β-TCP/SA/PDLLA) composite microspheres were successfully prepared using the liquid droplet method.
31142377	7	0	theme	setting	1158:1164	arg1	time					1166:1169	longer setting time	1151:1169	longer setting time	1151:1169	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	10	1	theme	clinical	1807:1814	arg1	application					1816:1826	clinical application	1807:1826	clinical application	1807:1826	This new bio-composite bone cement has potential in clinical application.
31142377	1	2	theme	high	268:271	arg1	generation					278:287	high heat generation	268:287	high heat generation	268:287	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	4	3	theme	inorganic	670:678	arg1	components					699:708	65 wt% inorganic and 35 wt% organic components	663:708	65 wt% inorganic and 35 wt% organic components	663:708	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	7	4	theme	curing	1131:1136	arg1	temperature					1138:1148	lower curing temperature	1125:1148	lower curing temperature	1125:1148	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	3	5	theme	bone	537:540	arg1	cement					542:547	this new bio-composite bone cement	514:547	this new bio-composite bone cement	514:547	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	11	6	from	stability	1904:1912	arg1	implantation					1960:1971	long-term implantation	1950:1971	long-term implantation	1950:1971	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	1	7	with	polymerization	296:309	arg1	tissue					342:347	bone tissue	337:347	bone tissue	337:347	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	4	8	theme	35 wt	684:688	arg1	%					689:689	35 wt%	684:689	35 wt%	684:689	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	5	9	theme	setting	890:896	arg1	time					898:901	setting time	890:901	setting time	890:901	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	8	10	theme	bio-composite	1402:1414	arg1	non-cytotoxic					1432:1444	non-cytotoxic	1432:1444	non-cytotoxic	1432:1444	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	8	10	theme	bio-composite	1402:1414	arg1	cement					1421:1426	the bio-composite bone cement	1398:1426	the bio-composite bone cement	1398:1426	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	7	11	theme	bone	1103:1106	arg1	cement					1108:1113	the bio-composite bone cement	1085:1113	the bio-composite bone cement	1085:1113	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	3	12	theme	better	555:560	arg1	osteo-integration					562:578	a better osteo-integration	553:578	a better osteo-integration than pure polymethyl methacrylate cement	553:619	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	4	13	theme	65 wt	663:667	arg1	%					668:668	65 wt%	663:668	65 wt%	663:668	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	11	14	theme	bone	1935:1938	arg1	cement					1940:1945	the bio-composite bone cement	1917:1945	the bio-composite bone cement	1917:1945	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	1	15	contain	has	238:240	arg1	it					230:231	it	230:231	it	230:231	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	1	15	contain	has	238:240	arg2	shortcomings					247:258	some shortcomings	242:258	some shortcomings such as high heat generation	242:287	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	1	15	contain	has	238:240	arg2	generation					278:287	high heat generation	268:287	high heat generation	268:287	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	5	16	theme	exothermic	858:867	arg1	changes					881:887	exothermic temperature changes	858:887	exothermic temperature changes	858:887	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	4	17	theme	tri-calcium	714:724	arg1	phosphate					726:734	tri-calcium phosphate	714:734	tri-calcium phosphate	714:734	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	3	18	theme	polymethyl	590:599	arg1	cement					614:619	pure polymethyl methacrylate cement	585:619	pure polymethyl methacrylate cement	585:619	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	5	19	theme	degradation	936:946	arg1	behaviors					948:956	degradation behaviors	936:956	degradation behaviors	936:956	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	8	20	dep	in	1455:1456	arg1	vivo					1458:1461	vivo	1458:1461	vivo	1458:1461	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	4	21	theme	organic	691:697	arg1	components					699:708	65 wt% inorganic and 35 wt% organic components	663:708	65 wt% inorganic and 35 wt% organic components	663:708	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	11	22	theme	future	1833:1838	arg1	studies					1840:1846	Our future studies	1829:1846	Our future studies	1829:1846	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	1	23	theme	bone	337:340	arg1	tissue					342:347	bone tissue	337:347	bone tissue	337:347	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	9	24	theme	phosphate/polymethyl	1583:1602	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	6	25	dep	in	1012:1013	arg1	vivo					1015:1018	vivo	1015:1018	vivo	1015:1018	Preliminary in vivo animal study was also performed.
31142377	8	26	theme	proliferation	1360:1372	arg1	tests					1374:1378	Cell proliferation tests	1355:1378	Cell proliferation tests	1355:1378	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	9	27	theme	bio-composites	1617:1630	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	6	28	theme	animal	1020:1025	arg1	study					1027:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study was also performed.
31142377	4	29	theme	degradable	774:783	arg1	additives					785:793	degradable additives	774:793	degradable additives	774:793	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	29	theme	degradable	774:783	arg1	contents					749:756	chitosan contents	740:756	chitosan contents	740:756	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	29	theme	degradable	774:783	arg1	composition					644:654	METHODS Physiological composition	622:654	METHODS Physiological composition	622:654	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	7	30	theme	compressive	1229:1239	arg1	modulus					1249:1255	lower compressive Young's modulus	1223:1255	lower compressive Young's modulus	1223:1255	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	4	31	dep	components	699:708	arg1	i.e.					657:660	i.e.	657:660	i.e.	657:660	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	6	32	theme	in	1012:1013	arg1	study					1027:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study was also performed.
31142377	0	33	theme	in	112:113	arg1	study					120:124	an in vitro and in vivo study	96:124	better osteo-integration: an in vitro and in vivo study	70:124	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	1	34	theme	Polymethylmethacrylate	138:159	arg1	cement					166:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	8	35	theme	bone	1416:1419	arg1	non-cytotoxic					1432:1444	non-cytotoxic	1432:1444	non-cytotoxic	1432:1444	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	8	35	theme	bone	1416:1419	arg1	cement					1421:1426	the bio-composite bone cement	1398:1426	the bio-composite bone cement	1398:1426	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	2	36	theme	bone	381:384	arg1	cement					386:391	a bio-composite bone cement	365:391	a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement	365:474	In this study, a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement was developed.
31142377	1	37	theme	heat	273:276	arg1	generation					278:287	high heat generation	268:287	high heat generation	268:287	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	5	38	theme	in	963:964	arg1	cytotoxicity					972:983	in vitro cytotoxicity	963:983	in vitro cytotoxicity	963:983	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	10	39	theme	bone	1778:1781	arg1	cement					1783:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement has potential in clinical application.
31142377	0	40	theme	phosphate	25:33	arg1	cement					54:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	0	41	dep	osteo-integration	77:93	arg1	study					120:124	an in vitro and in vivo study	96:124	better osteo-integration: an in vitro and in vivo study	70:124	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	7	42	theme	methacrylate	1334:1345	arg1	cement					1347:1352	pure polymethyl methacrylate cement	1318:1352	pure polymethyl methacrylate cement	1318:1352	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	10	43	theme	new	1760:1762	arg1	cement					1783:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement has potential in clinical application.
31142377	2	44	theme	tri-calcium	405:415	arg1	phosphate					417:425	tri-calcium phosphate	405:425	tri-calcium phosphate	405:425	In this study, a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement was developed.
31142377	0	45	theme	bone	49:52	arg1	cement					54:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	7	46	theme	weight	1179:1184	arg1	loss					1186:1189	higher weight loss	1172:1189	higher weight loss	1172:1189	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	7	47	theme	pure	1318:1321	arg1	cement					1347:1352	pure polymethyl methacrylate cement	1318:1352	pure polymethyl methacrylate cement	1318:1352	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	2	48	theme	bone	464:467	arg1	cement					469:474	acrylic bone cement	456:474	acrylic bone cement	456:474	In this study, a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement was developed.
31142377	9	49	theme	modified	1553:1560	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	4	50	theme	acrylic	806:812	arg1	cement					819:824	acrylic bone cement	806:824	acrylic bone cement	806:824	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	51	theme	Physiological	630:642	arg1	additives					785:793	degradable additives	774:793	degradable additives	774:793	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	51	theme	Physiological	630:642	arg1	contents					749:756	chitosan contents	740:756	chitosan contents	740:756	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	51	theme	Physiological	630:642	arg1	composition					644:654	METHODS Physiological composition	622:654	METHODS Physiological composition	622:654	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	7	52	theme	Young	1241:1245	arg1	modulus					1249:1255	lower compressive Young's modulus	1223:1255	lower compressive Young's modulus	1223:1255	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	7	53	theme	longer	1151:1156	arg1	time					1166:1169	longer setting time	1151:1169	longer setting time	1151:1169	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	1	54	with	integration	320:330	arg1	tissue					342:347	bone tissue	337:347	bone tissue	337:347	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	7	55	theme	ultimate	1262:1269	arg1	strength					1283:1290	ultimate compressive strength	1262:1290	ultimate compressive strength	1262:1290	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	7	56	dep	RESULTS	1053:1059	arg1	results					1065:1071	The results	1061:1071	RESULTS The results	1053:1071	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	9	57	theme	methacrylate	1604:1615	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	1	58	contain	has	173:175	arg1	cement					166:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	1	58	contain	has	173:175	arg2	applications					190:201	applications	190:201	applications	190:201	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	1	58	contain	has	173:175	arg2	variety					179:185	a variety	177:185	a variety of applications	177:201	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	7	59	theme	lower	1125:1129	arg1	temperature					1138:1148	lower curing temperature	1125:1148	lower curing temperature	1125:1148	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	3	60	theme	bio-composite	523:535	arg1	cement					542:547	this new bio-composite bone cement	514:547	this new bio-composite bone cement	514:547	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	5	61	theme	temperature	869:879	arg1	changes					881:887	exothermic temperature changes	858:887	exothermic temperature changes	858:887	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	1	62	theme	applications	190:201	arg1	applications					190:201	applications	190:201	applications	190:201	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	1	62	theme	applications	190:201	arg1	variety					179:185	a variety	177:185	a variety of applications	177:201	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	6	63	theme	Preliminary	1000:1010	arg1	study					1027:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study	1000:1031	Preliminary in vivo animal study was also performed.
31142377	7	64	theme	bio-composite	1089:1101	arg1	cement					1108:1113	the bio-composite bone cement	1085:1113	the bio-composite bone cement	1085:1113	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	1	65	theme	orthopedic	206:215	arg1	surgery					217:223	orthopedic surgery	206:223	orthopedic surgery	206:223	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	10	66	contain	has	1790:1792	arg1	cement					1783:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement has potential in clinical application.
31142377	10	66	contain	has	1790:1792	arg2	potential					1794:1802	potential	1794:1802	potential	1794:1802	This new bio-composite bone cement has potential in clinical application.
31142377	8	67	theme	Cell	1355:1358	arg1	tests					1374:1378	Cell proliferation tests	1355:1378	Cell proliferation tests	1355:1378	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	1	68	theme	bone	161:164	arg1	cement					166:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	3	69	theme	methacrylate	601:612	arg1	cement					614:619	pure polymethyl methacrylate cement	585:619	pure polymethyl methacrylate cement	585:619	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	11	70	theme	cement	1940:1945	arg1	stability					1904:1912	the stability	1900:1912	the stability of the bio-composite bone cement in long-term implantation	1900:1971	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	3	71	theme	pure	585:588	arg1	cement					614:619	pure polymethyl methacrylate cement	585:619	pure polymethyl methacrylate cement	585:619	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	11	72	theme	bio-composite	1921:1933	arg1	cement					1940:1945	the bio-composite bone cement	1917:1945	the bio-composite bone cement	1917:1945	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	1	73	theme	BACKGROUND	127:136	arg1	cement					166:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement	127:171	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	4	74	theme	phosphate	726:734	arg1	additives					785:793	degradable additives	774:793	degradable additives	774:793	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	74	theme	phosphate	726:734	arg1	contents					749:756	chitosan contents	740:756	chitosan contents	740:756	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	74	theme	phosphate	726:734	arg1	composition					644:654	METHODS Physiological composition	622:654	METHODS Physiological composition	622:654	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	5	75	theme	properties	839:848	arg1	series					829:834	A series	827:834	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity	827:983	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	9	76	theme	chitosan/tri-calcium	1562:1581	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	0	77	theme	in	99:100	arg1	study					120:124	an in vitro and in vivo study	96:124	better osteo-integration: an in vitro and in vivo study	70:124	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	5	78	theme	bio-mechanical	904:917	arg1	characteristics					919:933	bio-mechanical characteristics	904:933	bio-mechanical characteristics	904:933	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	5	79	dep	in	963:964	arg1	vitro					966:970	vitro	966:970	vitro	966:970	A series of properties such as exothermic temperature changes, setting time, bio-mechanical characteristics, degradation behaviors, and in vitro cytotoxicity were examined.
31142377	9	80	theme	bone	1632:1635	arg1	cement					1637:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement	1549:1642	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	0	81	dep	in	112:113	arg1	vivo					115:118	vivo	115:118	vivo	115:118	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	11	82	theme	long-term	1862:1870	arg1	implantation					1872:1883	long-term implantation	1862:1883	long-term implantation	1862:1883	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	9	83	dep	cell	1728:1731	arg1	adherence					1733:1741	adherence	1733:1741	adherence	1733:1741	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	0	84	theme	chitosan/tri-calcium	4:23	arg1	cement					54:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	0	85	dep	in	99:100	arg1	vitro					102:106	vitro	102:106	vitro	102:106	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	4	86	theme	chitosan	740:747	arg1	additives					785:793	degradable additives	774:793	degradable additives	774:793	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	86	theme	chitosan	740:747	arg1	contents					749:756	chitosan contents	740:756	chitosan contents	740:756	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	86	theme	chitosan	740:747	arg1	composition					644:654	METHODS Physiological composition	622:654	METHODS Physiological composition	622:654	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	2	87	theme	bio-composite	367:379	arg1	cement					386:391	a bio-composite bone cement	365:391	a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement	365:474	In this study, a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement was developed.
31142377	0	88	theme	bio-composite	35:47	arg1	cement					54:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement	0:59	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	7	89	theme	polymethyl	1323:1332	arg1	cement					1347:1352	pure polymethyl methacrylate cement	1318:1352	pure polymethyl methacrylate cement	1318:1352	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	10	90	theme	bio-composite	1764:1776	arg1	cement					1783:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement	1755:1788	This new bio-composite bone cement has potential in clinical application.
31142377	7	91	theme	higher	1172:1177	arg1	loss					1186:1189	higher weight loss	1172:1189	higher weight loss	1172:1189	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	9	92	theme	rougher	1683:1689	arg1	surfaces					1691:1698	rougher surfaces	1683:1698	rougher surfaces that would be beneficial to cell adherence and growth	1683:1752	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	0	93	theme	better	70:75	arg1	osteo-integration					77:93	better osteo-integration	70:93	better osteo-integration: an in vitro and in vivo study	70:124	The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study.
31142377	1	94	theme	poor	315:318	arg1	integration					320:330	poor integration	315:330	poor integration with bone tissue	315:347	BACKGROUND Polymethylmethacrylate bone cement has a variety of applications in orthopedic surgery, but it also has some shortcomings such as high heat generation during polymerization and poor integration with bone tissue.
31142377	2	95	theme	acrylic	456:462	arg1	cement					469:474	acrylic bone cement	456:474	acrylic bone cement	456:474	In this study, a bio-composite bone cement composed of tri-calcium phosphate and chitosan as additives to acrylic bone cement was developed.
31142377	3	96	contain	has	549:551	arg1	cement					542:547	this new bio-composite bone cement	514:547	this new bio-composite bone cement	514:547	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	3	96	contain	has	549:551	arg2	osteo-integration					562:578	a better osteo-integration	553:578	a better osteo-integration than pure polymethyl methacrylate cement	553:619	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
31142377	9	97	dep	CONCLUSIONS	1510:1520	arg1	indicated					1534:1542	indicated	1534:1542	indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth	1534:1752	CONCLUSIONS The results indicated that the modified chitosan/tri-calcium phosphate/polymethyl methacrylate bio-composites bone cement could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.
31142377	4	98	theme	METHODS	622:628	arg1	additives					785:793	degradable additives	774:793	degradable additives	774:793	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	98	theme	METHODS	622:628	arg1	contents					749:756	chitosan contents	740:756	chitosan contents	740:756	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	4	98	theme	METHODS	622:628	arg1	composition					644:654	METHODS Physiological composition	622:654	METHODS Physiological composition	622:654	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	8	99	theme	in	1455:1456	arg1	tests					1463:1467	the in vivo tests	1451:1467	the in vivo tests	1451:1467	Cell proliferation tests demonstrated that the bio-composite bone cement was non-cytotoxic, and the in vivo tests revealed that was more osteo-conductive.
31142377	11	100	theme	long-term	1950:1958	arg1	implantation					1960:1971	long-term implantation	1950:1971	long-term implantation	1950:1971	Our future studies will focus on long-term implantation to investigate the stability of the bio-composite bone cement in long-term implantation.
31142377	7	101	theme	compressive	1271:1281	arg1	strength					1283:1290	ultimate compressive strength	1262:1290	ultimate compressive strength	1262:1290	RESULTS The results showed that the bio-composite bone cement exhibited lower curing temperature, longer setting time, higher weight loss and porosity after degradation, lower compressive Young's modulus, and ultimate compressive strength as compared with those of pure polymethyl methacrylate cement.
31142377	4	102	theme	bone	814:817	arg1	cement					819:824	acrylic bone cement	806:824	acrylic bone cement	806:824	METHODS Physiological composition, i.e., 65 wt% inorganic and 35 wt% organic components, of tri-calcium phosphate and chitosan contents was selected as degradable additives to replace acrylic bone cement.
31142377	3	103	theme	new	519:521	arg1	cement					542:547	this new bio-composite bone cement	514:547	this new bio-composite bone cement	514:547	Our hypothesis is that this new bio-composite bone cement has a better osteo-integration than pure polymethyl methacrylate cement.
30468813	5	0	theme	sorption	694:701	arg1	capacities					703:712	sorption capacities	694:712	sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g	694:795	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	8	1	theme	oil/water	1268:1276	arg1	emulsions					1278:1286	oil/water emulsions	1268:1286	oil/water emulsions	1268:1286	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	1	2	theme	hydrophilic	120:130	arg1	cryogels					157:164	hydrophilic, hydrophobic and layered cryogels	120:164	hydrophilic, hydrophobic and layered cryogels	120:164	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	4	3	theme	Cp	529:530	arg1	reduction					516:524	The reduction	512:524	The reduction of Cp and Φ	512:536	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	8	4	theme	hydrophobic/hydrophilic	1142:1164	arg1	"					1172:1172	alternated hydrophobic/hydrophilic "Janus"	1131:1172	alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels	1131:1203	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	7	5	theme	estrogenic	1074:1083	arg1	pollutant					1085:1093	another estrogenic pollutant	1066:1093	another estrogenic pollutant	1066:1093	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	7	5	theme	estrogenic	1074:1083	arg1	A					1063:1063	bisphenol A	1053:1063	bisphenol A	1053:1063	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	8	6	theme	"	1172:1172	arg1	family					1121:1126	A new family	1115:1126	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels	1115:1203	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	2	7	theme	mold	304:307	arg1	diameter					309:316	mold diameter	304:316	mold diameter (Φ)	304:320	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	7	theme	mold	304:307	arg1	Φ					319:319	Φ	319:319	Φ	319:319	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	6	8	theme	liquid	932:937	arg1	media					939:943	liquid media	932:943	liquid media than the hydrophilic cryogels	932:973	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	3	9	theme	stiffer	491:497	arg1	cryogels					502:509	stiffer XG cryogels	491:509	stiffer XG cryogels	491:509	The addition of acetic acid to the precursor led to stiffer XG cryogels.
30468813	3	10	theme	XG	499:500	arg1	cryogels					502:509	stiffer XG cryogels	491:509	stiffer XG cryogels	491:509	The addition of acetic acid to the precursor led to stiffer XG cryogels.
30468813	5	11	theme	hydrophobic	656:666	arg1	cryogels					668:675	hydrophobic cryogels	656:675	hydrophobic cryogels	656:675	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	2	12	theme	polymer	252:258	arg1	Cp					275:276	Cp	275:276	Cp	275:276	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	12	theme	polymer	252:258	arg1	concentration					260:272	polymer concentration	252:272	polymer concentration (Cp)	252:277	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	0	13	theme	Hydrophilic	0:10	arg1	xanthan					47:53	Hydrophilic, hydrophobic, Janus and multilayer xanthan	0:53	xanthan	47:53	Hydrophilic, hydrophobic, Janus and multilayer xanthan based cryogels.
30468813	5	14	theme	5.2 ± 0.3 g/g	783:795	arg1	capacities					703:712	sorption capacities	694:712	sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g	694:795	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	8	15	theme	new	1117:1119	arg1	family					1121:1126	A new family	1115:1126	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels	1115:1203	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	6	16	theme	XG	828:829	arg1	cryogels					831:838	The hydrophobic XG cryogels	812:838	The hydrophobic XG cryogels	812:838	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	6	16	theme	XG	828:829	arg1	efficient					863:871	efficient	863:871	efficient	863:871	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	8	17	theme	emulsions	1278:1286	arg1	mixtures					1232:1239	mixtures	1232:1239	mixtures of diesel oil and water or oil/water emulsions	1232:1286	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	1	18	theme	hydrophobic	133:143	arg1	cryogels					157:164	hydrophilic, hydrophobic and layered cryogels	120:164	hydrophilic, hydrophobic and layered cryogels	120:164	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	5	19	theme	mineral	726:732	arg1	oils					748:751	diesel, mineral and sunflower oils	718:751	diesel, mineral and sunflower oils	718:751	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	6	20	theme	hydrophobic	816:826	arg1	cryogels					831:838	The hydrophobic XG cryogels	812:838	The hydrophobic XG cryogels	812:838	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	6	20	theme	hydrophobic	816:826	arg1	efficient					863:871	efficient	863:871	efficient	863:871	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	6	21	theme	estrogenic	905:914	arg1	estradiol					891:899	ethinyl estradiol	883:899	ethinyl estradiol	883:899	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	6	21	theme	estrogenic	905:914	arg1	pollutant					916:924	an estrogenic pollutant	902:924	an estrogenic pollutant	902:924	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	2	22	from	diameter	309:316	arg1	gel					296:298	the precursor gel	282:298	the precursor gel	282:298	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	1	23	theme	Xanthan	71:77	arg1	XG					84:85	XG	84:85	XG	84:85	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	1	23	theme	Xanthan	71:77	arg1	gum					79:81	Xanthan gum	71:81	Xanthan gum (XG)	71:86	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	9	24	theme	hydrophobic	1369:1379	arg1	layers					1397:1402	the hydrophobic and hydrophilic layers	1365:1402	the hydrophobic and hydrophilic layers	1365:1402	The "Janus" like and multilayered cryogels remained at the interface, where the hydrophobic and hydrophilic layers adsorbed oil and water selectively.
30468813	2	25	theme	XG	409:410	arg1	cryogels					412:419	resulting hydrophilic XG cryogels	387:419	resulting hydrophilic XG cryogels	387:419	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	9	26	theme	hydrophilic	1385:1395	arg1	layers					1397:1402	the hydrophobic and hydrophilic layers	1365:1402	the hydrophobic and hydrophilic layers	1365:1402	The "Janus" like and multilayered cryogels remained at the interface, where the hydrophobic and hydrophilic layers adsorbed oil and water selectively.
30468813	7	27	theme	hydrophobic	992:1002	arg1	cryogels					1007:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	1	28	theme	layered	149:155	arg1	cryogels					157:164	hydrophilic, hydrophobic and layered cryogels	120:164	hydrophilic, hydrophobic and layered cryogels	120:164	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	2	29	theme	hydrophilic	397:407	arg1	cryogels					412:419	resulting hydrophilic XG cryogels	387:419	resulting hydrophilic XG cryogels	387:419	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	7	30	theme	liquid	1101:1106	arg1	media					1108:1112	liquid media	1101:1112	liquid media	1101:1112	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	5	31	theme	sunflower	738:746	arg1	oils					748:751	diesel, mineral and sunflower oils	718:751	diesel, mineral and sunflower oils	718:751	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	1	32	theme	cryogels	157:164	arg1	creation					108:115	the creation	104:115	the creation of hydrophilic, hydrophobic and layered cryogels	104:164	Xanthan gum (XG) was applied for the creation of hydrophilic, hydrophobic and layered cryogels.
30468813	2	33	theme	resulting	387:395	arg1	cryogels					412:419	resulting hydrophilic XG cryogels	387:419	resulting hydrophilic XG cryogels	387:419	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	34	with	correlation	180:190	arg1	properties					344:353	physicochemical properties	328:353	physicochemical properties	328:353	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	34	with	correlation	180:190	arg1	parameters					373:382	morphological parameters	359:382	morphological parameters	359:382	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	8	35	theme	Janus	1167:1171	arg1	"					1172:1172	alternated hydrophobic/hydrophilic "Janus"	1131:1172	alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels	1131:1203	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	4	36	theme	cryogels	567:574	arg1	area					584:587	the cryogels surface area	563:587	the cryogels surface area	563:587	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	8	37	theme	alternated	1131:1140	arg1	"					1172:1172	alternated hydrophobic/hydrophilic "Janus"	1131:1172	alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels	1131:1203	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	7	38	theme	similar	1026:1032	arg1	capacity					1034:1041	similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media	1026:1112	similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media	1026:1112	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	2	39	theme	morphological	359:371	arg1	parameters					373:382	morphological parameters	359:382	morphological parameters	359:382	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	3	40	theme	acid	462:465	arg1	addition					443:450	The addition	439:450	The addition of acetic acid to the precursor	439:482	The addition of acetic acid to the precursor led to stiffer XG cryogels.
30468813	7	41	theme	XG	1004:1005	arg1	cryogels					1007:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	0	42	dep	xanthan	47:53	arg1	multilayer					36:45	multilayer	36:45	multilayer	36:45	Hydrophilic, hydrophobic, Janus and multilayer xanthan based cryogels.
30468813	0	42	dep	xanthan	47:53	arg1	Janus					26:30	Janus	26:30	Janus	26:30	Hydrophilic, hydrophobic, Janus and multilayer xanthan based cryogels.
30468813	8	43	theme	oil	1251:1253	arg1	mixtures					1232:1239	mixtures	1232:1239	mixtures of diesel oil and water or oil/water emulsions	1232:1286	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	5	44	theme	16 ± 2 g/g	768:777	arg1	capacities					703:712	sorption capacities	694:712	sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g	694:795	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	2	45	theme	physicochemical	328:342	arg1	properties					344:353	physicochemical properties	328:353	physicochemical properties	328:353	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	9	46	theme	Janus	1294:1298	arg1	"					1299:1299	The "Janus"	1289:1299	The "Janus" like and multilayered cryogels	1289:1330	The "Janus" like and multilayered cryogels remained at the interface, where the hydrophobic and hydrophilic layers adsorbed oil and water selectively.
30468813	5	47	theme	diesel	718:723	arg1	oils					748:751	diesel, mineral and sunflower oils	718:751	diesel, mineral and sunflower oils	718:751	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	2	48	theme	synthesis	198:206	arg1	diameter					309:316	mold diameter	304:316	mold diameter (Φ)	304:320	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	48	theme	synthesis	198:206	arg1	composition					236:246	solvent composition	228:246	solvent composition	228:246	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	48	theme	synthesis	198:206	arg1	parameters					208:217	synthesis parameters	198:217	synthesis parameters	198:217	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	48	theme	synthesis	198:206	arg1	concentration					260:272	polymer concentration	252:272	polymer concentration (Cp)	252:277	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	4	49	theme	surface	576:582	arg1	area					584:587	the cryogels surface area	563:587	the cryogels surface area	563:587	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	6	50	theme	ethinyl	883:889	arg1	estradiol					891:899	ethinyl estradiol	883:899	ethinyl estradiol	883:899	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	6	50	theme	ethinyl	883:889	arg1	pollutant					916:924	an estrogenic pollutant	902:924	an estrogenic pollutant	902:924	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	4	51	from	increase	551:558	arg1	porosity					593:600	porosity	593:600	porosity	593:600	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	4	51	from	increase	551:558	arg1	area					584:587	the cryogels surface area	563:587	the cryogels surface area	563:587	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	9	52	dep	cryogels	1323:1330	arg1	multilayered					1310:1321	multilayered	1310:1321	multilayered	1310:1321	The "Janus" like and multilayered cryogels remained at the interface, where the hydrophobic and hydrophilic layers adsorbed oil and water selectively.
30468813	2	53	theme	cryogels	412:419	arg1	properties					344:353	physicochemical properties	328:353	physicochemical properties	328:353	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	53	theme	cryogels	412:419	arg1	parameters					373:382	morphological parameters	359:382	morphological parameters	359:382	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	5	54	theme	XG	609:610	arg1	cryogels					612:619	XG cryogels	609:619	XG cryogels	609:619	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	7	55	theme	bisphenol	1053:1061	arg1	pollutant					1085:1093	another estrogenic pollutant	1066:1093	another estrogenic pollutant	1066:1093	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	7	55	theme	bisphenol	1053:1061	arg1	A					1063:1063	bisphenol A	1053:1063	bisphenol A	1053:1063	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	3	56	theme	acetic	455:460	arg1	acid					462:465	acetic acid	455:465	acetic acid	455:465	The addition of acetic acid to the precursor led to stiffer XG cryogels.
30468813	8	57	dep	cryogels	1196:1203	arg1	multilayered					1183:1194	multilayered	1183:1194	multilayered	1183:1194	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	2	58	theme	solvent	228:234	arg1	composition					236:246	solvent composition	228:246	solvent composition	228:246	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	59	theme	precursor	286:294	arg1	gel					296:298	the precursor gel	282:298	the precursor gel	282:298	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	2	60	from	composition	236:246	arg1	gel					296:298	the precursor gel	282:298	the precursor gel	282:298	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	7	61	theme	Hydrophilic	976:986	arg1	cryogels					1007:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels	976:1014	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	8	62	theme	diesel	1244:1249	arg1	oil					1251:1253	diesel oil	1244:1253	diesel oil	1244:1253	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	2	63	from	concentration	260:272	arg1	gel					296:298	the precursor gel	282:298	the precursor gel	282:298	Firstly, the correlation among synthesis parameters, such as solvent composition and polymer concentration (Cp) in the precursor gel and mold diameter (Φ), with physicochemical properties and morphological parameters of resulting hydrophilic XG cryogels was investigated.
30468813	6	64	theme	hydrophilic	954:964	arg1	cryogels					966:973	the hydrophilic cryogels	950:973	the hydrophilic cryogels	950:973	The hydrophobic XG cryogels were considerably more efficient to remove ethinyl estradiol, an estrogenic pollutant, from liquid media than the hydrophilic cryogels.
30468813	5	65	theme	28 ± 2 g/g	756:765	arg1	capacities					703:712	sorption capacities	694:712	sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g	694:795	Then, XG cryogels were silanized in order to produce hydrophobic cryogels, which presented sorption capacities for diesel, mineral and sunflower oils of 28 ± 2 g/g, 16 ± 2 g/g and 5.2 ± 0.3 g/g, respectively.
30468813	8	66	theme	water	1259:1263	arg1	mixtures					1232:1239	mixtures	1232:1239	mixtures of diesel oil and water or oil/water emulsions	1232:1286	A new family of alternated hydrophobic/hydrophilic "Janus" like and multilayered cryogels was created and applied in mixtures of diesel oil and water or oil/water emulsions.
30468813	7	67	attach	remove	1046:1051	arg2	capacity					1034:1041	similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media	1026:1112	similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media	1026:1112	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	7	67	attach	remove	1046:1051	arg1	media					1108:1112	liquid media	1101:1112	liquid media	1101:1112	Hydrophilic and hydrophobic XG cryogels presented similar capacity to remove bisphenol A, another estrogenic pollutant, from liquid media.
30468813	4	68	theme	Φ	536:536	arg1	reduction					516:524	The reduction	512:524	The reduction of Cp and Φ	512:536	The reduction of Cp and Φ promoted the increase in the cryogels surface area and porosity.
30468813	0	69	dep	Hydrophilic	0:10	arg1	hydrophobic					13:23	hydrophobic	13:23	hydrophobic	13:23	Hydrophilic, hydrophobic, Janus and multilayer xanthan based cryogels.
30986464	8	0	theme	·OH	1291:1293	arg1	species					1282:1288	the reactive species	1269:1288	the reactive species	1269:1288	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	8	0	theme	·OH	1291:1293	arg1	radical					1295:1301	·OH radical	1291:1301	·OH radical	1291:1301	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	4	1	theme	UV-vis	741:746	arg1	DRS					748:750	UV-vis DRS	741:750	UV-vis DRS	741:750	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	4	1	theme	UV-vis	741:746	arg1	spectroscopy					727:738	The UV-visible diffusion reflectance spectroscopy	690:738	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS)	690:751	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	6	2	theme	enhanced	1035:1042	arg1	efficiency					1071:1080	enhanced photocatalytic degradation efficiency	1035:1080	enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue	1035:1117	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	1	3	theme	irradiation	264:274	arg1	presence					235:242	presence	235:242	presence of UV/visible light irradiation	235:274	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	5	4	theme	prepared	944:951	arg1	IPCA					953:956	the prepared IPCA	940:956	the prepared IPCA	940:956	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	0	5	from	synthesis	7:15	arg1	degradation					89:99	photocatalytic degradation	74:99	photocatalytic degradation of methylene blue	74:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	6	6	theme	blue	1114:1117	arg1	Methylene					1104:1112	Methylene blue	1104:1117	Methylene blue	1104:1117	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	8	7	theme	major	1332:1336	arg1	role					1338:1341	a major role	1330:1341	a major role	1330:1341	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	4	8	theme	gap	775:777	arg1	measurement					779:789	the band gap measurement	766:789	the band gap measurement	766:789	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	0	9	theme	blue	114:117	arg1	methylene					104:112	methylene blue	104:117	methylene blue	104:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	6	10	dep	efficiency	1071:1080	arg1	%					1087:1087	93.5%	1083:1087	93.5% in 40 min	1083:1097	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	8	11	theme	O2·-	1307:1310	arg1	radical					1312:1318	O2·- radical	1307:1318	O2·- radical	1307:1318	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	8	11	theme	O2·-	1307:1310	arg1	species					1282:1288	the reactive species	1269:1288	the reactive species	1269:1288	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	5	12	dep	dye	865:867	arg1	30 mL					870:874	30 mL	870:874	30 mL of 100 mg/L	870:886	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	1	13	used	used	178:181	arg2	IPCA					168:171	IPCA	168:171	IPCA	168:171	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	1	13	used	used	178:181	arg2	adsorbents					156:165	Integrated photocatalytic composite adsorbents	120:165	Integrated photocatalytic composite adsorbents (IPCA)	120:172	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	4	14	theme	diffusion	705:713	arg1	DRS					748:750	UV-vis DRS	741:750	UV-vis DRS	741:750	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	4	14	theme	diffusion	705:713	arg1	spectroscopy					727:738	The UV-visible diffusion reflectance spectroscopy	690:738	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS)	690:751	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	0	15	theme	methylene	104:112	arg1	degradation					89:99	photocatalytic degradation	74:99	photocatalytic degradation of methylene blue	74:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	0	16	from	influence	61:69	arg1	degradation					89:99	photocatalytic degradation	74:99	photocatalytic degradation of methylene blue	74:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	3	17	theme	resultant	609:617	arg1	composites					619:628	the resultant composites	605:628	the resultant composites	605:628	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	7	18	theme	MB	1214:1215	arg1	dye					1217:1219	MB dye	1214:1219	MB dye	1214:1219	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	5	19	theme	dye	865:867	arg1	solution					833:840	An aqueous solution	822:840	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L)	822:887	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	2	20	theme	same	362:365	arg1	time					367:370	the same time	358:370	the same time	358:370	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	21	theme	low	445:447	arg1	drawbacks					381:389	drawbacks	381:389	drawbacks like rapid recombination of photogenerated electrons	381:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	21	theme	low	445:447	arg1	absorptivity					449:460	low absorptivity	445:460	low absorptivity	445:460	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	22	theme	photogenerated	419:432	arg1	electrons					434:442	photogenerated electrons	419:442	photogenerated electrons	419:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	3	23	theme	integrated	516:525	arg1	Chitosan-La3+-Graphite					542:563	an integrated Photocatalytic Chitosan-La3+-Graphite	513:563	an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent	513:583	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	4	24	theme	UV-visible	694:703	arg1	DRS					748:750	UV-vis DRS	741:750	UV-vis DRS	741:750	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	4	24	theme	UV-visible	694:703	arg1	spectroscopy					727:738	The UV-visible diffusion reflectance spectroscopy	690:738	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS)	690:751	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	5	25	theme	aqueous	825:831	arg1	solution					833:840	An aqueous solution	822:840	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L)	822:887	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	2	26	from	time	367:370	arg1	features					317:324	all the existing features	300:324	all the existing features of individual components and at the same time	300:370	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	27	theme	components	340:349	arg1	features					317:324	all the existing features	300:324	all the existing features of individual components and at the same time	300:370	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	7	28	theme	porous	1246:1251	arg1	IPCA					1253:1256	highly porous IPCA	1239:1256	highly porous IPCA	1239:1256	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	1	29	theme	toxic	194:198	arg1	compounds					222:230	toxic organic and inorganic compounds	194:230	toxic organic and inorganic compounds	194:230	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	0	30	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of chitosan-La3+-graphite composite	0:51	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	6	31	theme	Chitosan-La3+-Graphite	963:984	arg1	CS-La-GR					997:1004	CS-La-GR	997:1004	CS-La-GR	997:1004	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	6	31	theme	Chitosan-La3+-Graphite	963:984	arg1	Composite					986:994	The Chitosan-La3+-Graphite Composite	959:994	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage)	959:1024	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	6	32	from	%	1087:1087	arg1	40 min					1092:1097	40 min	1092:1097	40 min	1092:1097	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	6	33	theme	photocatalytic	1044:1057	arg1	efficiency					1071:1080	enhanced photocatalytic degradation efficiency	1035:1080	enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue	1035:1117	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	2	34	theme	individual	329:338	arg1	components					340:349	individual components	329:349	individual components	329:349	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	1	35	theme	organic	200:206	arg1	compounds					222:230	toxic organic and inorganic compounds	194:230	toxic organic and inorganic compounds	194:230	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	0	36	theme	chitosan-La3+-graphite	20:41	arg1	composite					43:51	chitosan-La3+-graphite composite	20:51	chitosan-La3+-graphite composite	20:51	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	5	37	dep	blue	855:858	arg1	MB					861:862	MB	861:862	MB	861:862	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	4	38	used	used	757:760	arg2	spectroscopy					727:738	The UV-visible diffusion reflectance spectroscopy	690:738	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS)	690:751	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	4	38	used	used	757:760	arg2	DRS					748:750	UV-vis DRS	741:750	UV-vis DRS	741:750	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	6	39	theme	light	1128:1132	arg1	irradiation					1134:1144	UV light irradiation	1125:1144	UV light irradiation	1125:1144	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	5	40	used	used	893:896	arg2	solution					833:840	An aqueous solution	822:840	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L)	822:887	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	5	41	theme	IPCA	953:956	arg1	efficiency					926:935	the photocatalytic efficiency	907:935	the photocatalytic efficiency of the prepared IPCA	907:956	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	4	42	theme	band	770:773	arg1	measurement					779:789	the band gap measurement	766:789	the band gap measurement	766:789	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	1	43	theme	Integrated	120:129	arg1	adsorbents					156:165	Integrated photocatalytic composite adsorbents	120:165	Integrated photocatalytic composite adsorbents (IPCA)	120:172	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	1	43	theme	Integrated	120:129	arg1	IPCA					168:171	IPCA	168:171	IPCA	168:171	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	1	44	theme	inorganic	212:220	arg1	compounds					222:230	toxic organic and inorganic compounds	194:230	toxic organic and inorganic compounds	194:230	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	3	45	theme	Photocatalytic	527:540	arg1	Chitosan-La3+-Graphite					542:563	an integrated Photocatalytic Chitosan-La3+-Graphite	513:563	an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent	513:583	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	8	46	theme	composite	1400:1408	arg1	photocatalyst					1410:1422	composite photocatalyst	1400:1422	composite photocatalyst	1400:1422	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	3	47	theme	XRD	658:660	arg1	analysis					680:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	2	48	theme	rapid	396:400	arg1	recombination					402:414	rapid recombination	396:414	rapid recombination of photogenerated electrons	396:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	0	49	theme	composite	43:51	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of chitosan-La3+-graphite composite	0:51	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	0	49	theme	composite	43:51	arg1	influence					61:69	its influence	57:69	its influence in photocatalytic degradation of methylene blue	57:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	3	50	theme	SEM	676:678	arg1	analysis					680:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	2	51	theme	hindrance	466:474	arg1	effect					476:481	hindrance effect	466:481	hindrance effect of the photocatalyst	466:502	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	51	theme	hindrance	466:474	arg1	drawbacks					381:389	drawbacks	381:389	drawbacks like rapid recombination of photogenerated electrons	381:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	8	52	theme	species	1282:1288	arg1	ions					1320:1323	the reactive species, ·OH radical and O2·- radical ions	1269:1323	the reactive species, ·OH radical and O2·- radical ions	1269:1323	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	2	53	theme	electrons	434:442	arg1	recombination					402:414	rapid recombination	396:414	rapid recombination of photogenerated electrons	396:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	54	theme	photocatalyst	490:502	arg1	effect					476:481	hindrance effect	466:481	hindrance effect of the photocatalyst	466:502	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	54	theme	photocatalyst	490:502	arg1	drawbacks					381:389	drawbacks	381:389	drawbacks like rapid recombination of photogenerated electrons	381:442	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	2	54	theme	photocatalyst	490:502	arg1	absorptivity					449:460	low absorptivity	445:460	low absorptivity	445:460	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	5	55	theme	100 mg/L	879:886	arg1	30 mL					870:874	30 mL	870:874	30 mL of 100 mg/L	870:886	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	6	56	dep	Composite	986:994	arg1	100 mg					1008:1013	100 mg	1008:1013	100 mg of dosage	1008:1023	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	7	57	theme	stronger	1191:1198	arg1	adsorption					1200:1209	the stronger adsorption	1187:1209	the stronger adsorption of MB dye on the surface of highly porous IPCA	1187:1256	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	3	58	theme	FTIR	652:655	arg1	analysis					680:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	1	59	theme	photocatalytic	131:144	arg1	adsorbents					156:165	Integrated photocatalytic composite adsorbents	120:165	Integrated photocatalytic composite adsorbents (IPCA)	120:172	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	1	59	theme	photocatalytic	131:144	arg1	IPCA					168:171	IPCA	168:171	IPCA	168:171	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	6	60	theme	degradation	1059:1069	arg1	efficiency					1071:1080	enhanced photocatalytic degradation efficiency	1035:1080	enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue	1035:1117	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	7	61	from	adsorption	1200:1209	arg1	surface					1228:1234	the surface	1224:1234	the surface of highly porous IPCA	1224:1256	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	8	62	theme	photocatalytic	1350:1363	arg1	degradation					1365:1375	the photocatalytic degradation	1346:1375	the photocatalytic degradation of Methylene blue over composite photocatalyst	1346:1422	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	7	63	theme	IPCA	1253:1256	arg1	surface					1228:1234	the surface	1224:1234	the surface of highly porous IPCA	1224:1256	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	1	64	theme	composite	146:154	arg1	adsorbents					156:165	Integrated photocatalytic composite adsorbents	120:165	Integrated photocatalytic composite adsorbents (IPCA)	120:172	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	1	64	theme	composite	146:154	arg1	IPCA					168:171	IPCA	168:171	IPCA	168:171	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	8	65	theme	Methylene	1380:1388	arg1	degradation					1365:1375	the photocatalytic degradation	1346:1375	the photocatalytic degradation of Methylene blue over composite photocatalyst	1346:1422	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	8	66	theme	blue	1390:1393	arg1	Methylene					1380:1388	Methylene blue	1380:1393	Methylene blue	1380:1393	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	4	67	theme	reflectance	715:725	arg1	DRS					748:750	UV-vis DRS	741:750	UV-vis DRS	741:750	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	4	67	theme	reflectance	715:725	arg1	spectroscopy					727:738	The UV-visible diffusion reflectance spectroscopy	690:738	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS)	690:751	The UV-visible diffusion reflectance spectroscopy (UV-vis DRS) was used for the band gap measurement and it was found to be 3.2 eV.
30986464	3	68	theme	EDX	668:670	arg1	analysis					680:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	8	69	theme	reactive	1273:1280	arg1	radical					1312:1318	O2·- radical	1307:1318	O2·- radical	1307:1318	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	8	69	theme	reactive	1273:1280	arg1	species					1282:1288	the reactive species	1269:1288	the reactive species	1269:1288	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	8	69	theme	reactive	1273:1280	arg1	radical					1295:1301	·OH radical	1291:1301	·OH radical	1291:1301	Moreover, the reactive species, ·OH radical and O2·- radical ions play a major role in the photocatalytic degradation of Methylene blue over composite photocatalyst.
30986464	6	70	theme	dosage	1018:1023	arg1	100 mg					1008:1013	100 mg	1008:1013	100 mg of dosage	1008:1023	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	2	71	theme	existing	308:315	arg1	features					317:324	all the existing features	300:324	all the existing features of individual components and at the same time	300:370	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	5	72	theme	photocatalytic	911:924	arg1	efficiency					926:935	the photocatalytic efficiency	907:935	the photocatalytic efficiency of the prepared IPCA	907:956	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	0	73	theme	photocatalytic	74:87	arg1	degradation					89:99	photocatalytic degradation	74:99	photocatalytic degradation of methylene blue	74:117	Facile synthesis of chitosan-La3+-graphite composite and its influence in photocatalytic degradation of methylene blue.
30986464	3	74	theme	TGA	663:665	arg1	analysis					680:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	FTIR, XRD, TGA, EDX and SEM analysis	652:687	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	5	75	theme	blue	855:858	arg1	dye					865:867	methylene blue (MB) dye	845:867	methylene blue (MB) dye (30 mL of 100 mg/L)	845:887	An aqueous solution of methylene blue (MB) dye (30 mL of 100 mg/L) was used to study the photocatalytic efficiency of the prepared IPCA.
30986464	3	76	theme	adsorbent	575:583	arg1	Chitosan-La3+-Graphite					542:563	an integrated Photocatalytic Chitosan-La3+-Graphite	513:563	an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent	513:583	Herein, an integrated Photocatalytic Chitosan-La3+-Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR, XRD, TGA, EDX and SEM analysis.
30986464	1	77	theme	UV/visible	247:256	arg1	irradiation					264:274	UV/visible light irradiation	247:274	UV/visible light irradiation	247:274	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
30986464	7	78	theme	dye	1217:1219	arg1	adsorption					1200:1209	the stronger adsorption	1187:1209	the stronger adsorption of MB dye on the surface of highly porous IPCA	1187:1256	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	6	79	theme	UV	1125:1126	arg1	irradiation					1134:1144	UV light irradiation	1125:1144	UV light irradiation	1125:1144	The Chitosan-La3+-Graphite Composite (CS-La-GR) (100 mg of dosage) exhibits enhanced photocatalytic degradation efficiency (93.5% in 40 min) for Methylene blue under UV light irradiation.
30986464	7	80	theme	highest	1151:1157	arg1	due					1180:1182	due	1180:1182	due	1180:1182	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	7	80	theme	highest	1151:1157	arg1	photodegradation					1159:1174	The highest photodegradation	1147:1174	The highest photodegradation	1147:1174	The highest photodegradation was due to the stronger adsorption of MB dye on the surface of highly porous IPCA.
30986464	2	81	dep	preserves	290:298	arg1	overcome					372:379	overcome	372:379	overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst	372:502	The compound preserves all the existing features of individual components and at the same time overcome drawbacks like rapid recombination of photogenerated electrons, low absorptivity and hindrance effect of the photocatalyst.
30986464	1	82	theme	light	258:262	arg1	irradiation					264:274	UV/visible light irradiation	247:274	UV/visible light irradiation	247:274	Integrated photocatalytic composite adsorbents (IPCA) are used to degrade toxic organic and inorganic compounds in presence of UV/visible light irradiation.
29915530	4	0	theme	microscopy	586:595	arg1	combination					571:581	a combination	569:581	a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons	569:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	1	1	theme	excitatory	168:177	arg1	neurotransmission					179:195	excitatory neurotransmission	168:195	excitatory neurotransmission	168:195	N-methyl-D-aspartate receptors (NMDARs) play critical roles in both excitatory neurotransmission and synaptic plasticity.
29915530	0	2	theme	Receptors	89:97	arg1	Trafficking					30:40	Trafficking	30:40	Trafficking	30:40	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	0	2	theme	Receptors	89:97	arg1	Mobility					54:61	Surface Mobility	46:61	Surface Mobility	46:61	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	7	3	dep	1-deoxymannojirimycin	1458:1478	arg1	DMM					1481:1483	DMM	1481:1483	DMM	1481:1483	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	3	dep	1-deoxymannojirimycin	1458:1478	arg1	inhibitor					1489:1497	an inhibitor	1486:1497	an inhibitor of the formation of the hybrid/complex forms of N-glycans	1486:1555	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	4	4	from	biochemistry	598:609	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	4	from	biochemistry	598:609	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	5	theme	hippocampal	666:676	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	6	theme	biochemistry	598:609	arg1	combination					571:581	a combination	569:581	a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons	569:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	6	7	theme	NMDARs	1316:1321	arg1	delivery					1286:1293	the surface delivery	1274:1293	the surface delivery of GluN3A-containing NMDARs	1274:1321	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	2	8	theme	GluN1/GluN2	347:357	arg1	subunits					359:366	GluN1/GluN2 subunits	347:366	GluN1/GluN2 subunits	347:366	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	4	9	from	combination	571:581	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	9	from	combination	571:581	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	7	10	theme	formation	1506:1514	arg1	DMM					1481:1483	DMM	1481:1483	DMM	1481:1483	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	10	theme	formation	1506:1514	arg1	inhibitor					1489:1497	an inhibitor	1486:1497	an inhibitor of the formation of the hybrid/complex forms of N-glycans	1486:1555	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	6	11	with	patients	1175:1182	arg1	disorder					1202:1209	a congenital disorder	1189:1209	a congenital disorder of glycosylation	1189:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	4	12	from	microscopy	586:595	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	12	from	microscopy	586:595	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	5	13	theme	GluN3A	968:973	arg1	subunits					975:982	GluN3A subunits	968:982	GluN3A subunits	968:982	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	6	14	theme	congenital	1191:1200	arg1	disorder					1202:1209	a congenital disorder	1189:1209	a congenital disorder of glycosylation	1189:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	4	15	from	neurons	678:684	arg1	combination					571:581	a combination	569:581	a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons	569:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	6	16	attach	derived	1162:1168	arg2	fibroblasts					1150:1160	human fibroblasts	1144:1160	human fibroblasts derived from patients with a congenital disorder of glycosylation	1144:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	6	16	attach	derived	1162:1168	arg1	patients					1175:1182	patients	1175:1182	patients with a congenital disorder of glycosylation	1175:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	7	17	theme	hippocampal	1399:1409	arg1	neurons					1411:1417	hippocampal neurons	1399:1417	hippocampal neurons	1399:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	4	18	theme	GluN3A	821:826	arg1	subunit					828:834	the GluN3A subunit	817:834	the GluN3A subunit	817:834	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	6	19	theme	human	1144:1148	arg1	fibroblasts					1150:1160	human fibroblasts	1144:1160	human fibroblasts derived from patients with a congenital disorder of glycosylation	1144:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	5	20	theme	deglycosylation	912:926	arg1	analysis					945:952	deglycosylation and lectin-based analysis	912:952	analysis	945:952	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	7	21	theme	N-glycans	1547:1555	arg1	forms					1538:1542	the hybrid/complex forms	1519:1542	the hybrid/complex forms of N-glycans	1519:1555	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	4	22	theme	NMDARs	891:896	arg1	delivery					861:868	surface delivery	853:868	surface delivery of GluN3A-containing NMDARs	853:896	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	7	23	theme	hybrid/complex	1523:1536	arg1	forms					1538:1542	the hybrid/complex forms	1519:1542	the hybrid/complex forms of N-glycans	1519:1555	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	1	24	theme	synaptic	201:208	arg1	plasticity					210:219	synaptic plasticity	201:219	synaptic plasticity	201:219	N-methyl-D-aspartate receptors (NMDARs) play critical roles in both excitatory neurotransmission and synaptic plasticity.
29915530	8	25	theme	new	1632:1634	arg1	insight					1636:1642	new insight	1632:1642	new insight regarding the mechanisms by which neurons can regulate NMDAR trafficking and function	1632:1728	These findings provide new insight regarding the mechanisms by which neurons can regulate NMDAR trafficking and function.
29915530	3	26	theme	GluN1/GluN2	398:408	arg1	receptors					410:418	GluN1/GluN2 receptors	398:418	GluN1/GluN2 receptors	398:418	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	1	27	theme	N-methyl-D-aspartate	100:119	arg1	receptors					121:129	N-methyl-D-aspartate receptors	100:129	N-methyl-D-aspartate receptors (NMDARs)	100:138	N-methyl-D-aspartate receptors (NMDARs) play critical roles in both excitatory neurotransmission and synaptic plasticity.
29915530	1	27	theme	N-methyl-D-aspartate	100:119	arg1	NMDARs					132:137	NMDARs	132:137	NMDARs	132:137	N-methyl-D-aspartate receptors (NMDARs) play critical roles in both excitatory neurotransmission and synaptic plasticity.
29915530	7	28	theme	surface	1351:1357	arg1	mobility					1359:1366	the surface mobility	1347:1366	the surface mobility of GluN3A-containing NMDARs in hippocampal neurons	1347:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	5	29	theme	lectin-based	932:943	arg1	analysis					945:952	deglycosylation and lectin-based analysis	912:952	analysis	945:952	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	3	30	theme	limited	463:469	arg1	information					471:481	limited information	463:481	limited information	463:481	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	7	31	with	incubation	1442:1451	arg1	1-deoxymannojirimycin					1458:1478	1-deoxymannojirimycin	1458:1478	1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans)	1458:1556	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	32	from	mobility	1359:1366	arg1	neurons					1411:1417	hippocampal neurons	1399:1417	hippocampal neurons	1399:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	6	33	theme	N-glycan	1234:1241	arg1	remodeling					1243:1252	N-glycan remodeling	1234:1252	N-glycan remodeling	1234:1252	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	4	34	from	lines	652:656	arg1	combination					571:581	a combination	569:581	a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons	569:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	35	theme	cell	647:650	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	36	dep	residues	716:723	arg1	N203					726:729	N203	726:729	N203	726:729	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	36	dep	residues	716:723	arg1	residues					716:723	two asparagine residues	701:723	two asparagine residues (N203 and N368) in the GluN1 subunit	701:760	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	36	dep	residues	716:723	arg1	N368					735:738	N368	735:738	N368	735:738	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	37	dep	residues	783:790	arg1	residues					783:790	three asparagine residues	766:790	three asparagine residues (N145, N264 and N275) in the GluN3A subunit	766:834	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	37	dep	residues	783:790	arg1	N275					808:811	N275	808:811	N275	808:811	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	37	dep	residues	783:790	arg1	N264					799:802	N264	799:802	N264	799:802	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	38	theme	rat	662:664	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	5	39	theme	N-glycans	1068:1076	arg1	forms					1059:1063	hybrid/complex forms	1044:1063	hybrid/complex forms of N-glycans	1044:1076	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	5	40	theme	hybrid/complex	1044:1057	arg1	forms					1059:1063	hybrid/complex forms	1044:1063	hybrid/complex forms of N-glycans	1044:1076	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	2	41	contain	containing	229:238	arg2	subunit					267:273	the nonconventional GluN3A subunit	240:273	the nonconventional GluN3A subunit	240:273	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	2	41	contain	containing	229:238	arg1	NMDARs					222:227	NMDARs	222:227	NMDARs containing the nonconventional GluN3A subunit	222:273	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	0	42	theme	Surface	46:52	arg1	Mobility					54:61	Surface Mobility	46:61	Surface Mobility	46:61	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	7	43	theme	NMDARs	1389:1394	arg1	mobility					1359:1366	the surface mobility	1347:1366	the surface mobility of GluN3A-containing NMDARs in hippocampal neurons	1347:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	44	theme	GluN3A-containing	1371:1387	arg1	NMDARs					1389:1394	GluN3A-containing NMDARs	1371:1394	GluN3A-containing NMDARs in hippocampal neurons	1371:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	2	45	theme	different	280:288	arg1	properties					301:310	different functional properties	280:310	different functional properties	280:310	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	4	46	from	residues	716:723	arg1	subunit					828:834	the GluN3A subunit	817:834	the GluN3A subunit	817:834	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	46	from	residues	716:723	arg1	subunit					754:760	the GluN1 subunit	744:760	the GluN1 subunit	744:760	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	6	47	theme	inhibitors	1118:1127	arg1	panel					1109:1113	a panel	1107:1113	a panel of inhibitors	1107:1127	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	4	48	theme	electrophysiology	616:632	arg1	combination					571:581	a combination	569:581	a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons	569:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	49	theme	asparagine	705:714	arg1	N203					726:729	N203	726:729	N203	726:729	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	49	theme	asparagine	705:714	arg1	residues					716:723	two asparagine residues	701:723	two asparagine residues (N203 and N368) in the GluN1 subunit	701:760	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	49	theme	asparagine	705:714	arg1	N368					735:738	N368	735:738	N368	735:738	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	3	50	theme	GluN3A-containing	537:553	arg1	NMDARs					555:560	GluN3A-containing NMDARs	537:560	GluN3A-containing NMDARs	537:560	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	4	51	from	electrophysiology	616:632	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	51	from	electrophysiology	616:632	arg1	neurons					678:684	rat hippocampal neurons	662:684	rat hippocampal neurons	662:684	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	8	52	theme	NMDAR	1699:1703	arg1	trafficking					1705:1715	NMDAR trafficking	1699:1715	NMDAR trafficking	1699:1715	These findings provide new insight regarding the mechanisms by which neurons can regulate NMDAR trafficking and function.
29915530	3	53	theme	Previous	369:376	arg1	studies					378:384	Previous studies	369:384	Previous studies	369:384	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	4	54	theme	GluN3A-containing	873:889	arg1	NMDARs					891:896	GluN3A-containing NMDARs	873:896	GluN3A-containing NMDARs	873:896	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	7	55	from	NMDARs	1389:1394	arg1	neurons					1411:1417	hippocampal neurons	1399:1417	hippocampal neurons	1399:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	6	56	theme	glycosylation	1214:1226	arg1	disorder					1202:1209	a congenital disorder	1189:1209	a congenital disorder of glycosylation	1189:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	6	57	dep	found	1087:1091	arg1	studying					1135:1142	studying	1135:1142	studying human fibroblasts derived from patients with a congenital disorder of glycosylation	1135:1226	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	6	57	dep	found	1087:1091	arg1	panel					1109:1113	a panel	1107:1113	a panel of inhibitors	1107:1127	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	4	58	theme	mammalian	637:645	arg1	lines					652:656	mammalian cell lines	637:656	mammalian cell lines	637:656	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	2	59	theme	nonconventional	244:258	arg1	subunit					267:273	the nonconventional GluN3A subunit	240:273	the nonconventional GluN3A subunit	240:273	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	2	60	theme	GluN3A	260:265	arg1	subunit					267:273	the nonconventional GluN3A subunit	240:273	the nonconventional GluN3A subunit	240:273	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	4	61	theme	asparagine	772:781	arg1	residues					783:790	three asparagine residues	766:790	three asparagine residues (N145, N264 and N275) in the GluN3A subunit	766:834	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	61	theme	asparagine	772:781	arg1	N275					808:811	N275	808:811	N275	808:811	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	61	theme	asparagine	772:781	arg1	N264					799:802	N264	799:802	N264	799:802	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	6	62	theme	GluN3A-containing	1298:1314	arg1	NMDARs					1316:1321	GluN3A-containing NMDARs	1298:1321	GluN3A-containing NMDARs	1298:1321	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	0	63	dep	Trafficking	30:40	arg1	the					26:28	the	26:28	the	26:28	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	4	64	theme	surface	853:859	arg1	delivery					861:868	surface delivery	853:868	surface delivery of GluN3A-containing NMDARs	853:896	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	1	65	theme	critical	145:152	arg1	roles					154:158	critical roles	145:158	critical roles	145:158	N-methyl-D-aspartate receptors (NMDARs) play critical roles in both excitatory neurotransmission and synaptic plasticity.
29915530	5	66	contain	contain	984:990	arg2	structures					1022:1031	extensively modified N-glycan structures	992:1031	extensively modified N-glycan structures	992:1031	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	5	66	contain	contain	984:990	arg1	subunits					975:982	GluN3A subunits	968:982	GluN3A subunits	968:982	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	5	66	contain	contain	984:990	arg2	forms					1059:1063	hybrid/complex forms	1044:1063	hybrid/complex forms of N-glycans	1044:1076	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	7	67	theme	specific	1591:1598	arg1	lectins					1600:1606	specific lectins	1591:1606	specific lectins	1591:1606	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	68	theme	forms	1538:1542	arg1	formation					1506:1514	the formation	1502:1514	the formation of the hybrid/complex forms of N-glycans	1502:1555	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	7	69	from	neurons	1411:1417	arg1	mobility					1359:1366	the surface mobility	1347:1366	the surface mobility of GluN3A-containing NMDARs in hippocampal neurons	1347:1417	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	5	70	theme	N-glycan	1013:1020	arg1	structures					1022:1031	extensively modified N-glycan structures	992:1031	extensively modified N-glycan structures	992:1031	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	5	70	theme	N-glycan	1013:1020	arg1	forms					1059:1063	hybrid/complex forms	1044:1063	hybrid/complex forms of N-glycans	1044:1076	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	4	71	from	residues	783:790	arg1	subunit					828:834	the GluN3A subunit	817:834	the GluN3A subunit	817:834	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	4	71	from	residues	783:790	arg1	subunit					754:760	the GluN1 subunit	744:760	the GluN1 subunit	744:760	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
29915530	5	72	theme	modified	1004:1011	arg1	structures					1022:1031	extensively modified N-glycan structures	992:1031	extensively modified N-glycan structures	992:1031	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	5	72	theme	modified	1004:1011	arg1	forms					1059:1063	hybrid/complex forms	1044:1063	hybrid/complex forms of N-glycans	1044:1076	Furthermore, deglycosylation and lectin-based analysis revealed that GluN3A subunits contain extensively modified N-glycan structures, including hybrid/complex forms of N-glycans.
29915530	0	73	theme	NMDA	84:87	arg1	Receptors					89:97	GluN3A-Containing NMDA Receptors	66:97	GluN3A-Containing NMDA Receptors	66:97	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	7	74	theme	lectins	1600:1606	arg1	presence					1579:1586	the presence	1575:1586	the presence of specific lectins	1575:1606	Finally, we found that the surface mobility of GluN3A-containing NMDARs in hippocampal neurons is increased following incubation with 1-deoxymannojirimycin (DMM, an inhibitor of the formation of the hybrid/complex forms of N-glycans) and decreased in the presence of specific lectins.
29915530	6	75	theme	surface	1278:1284	arg1	delivery					1286:1293	the surface delivery	1274:1293	the surface delivery of GluN3A-containing NMDARs	1274:1321	We also found (either using a panel of inhibitors or by studying human fibroblasts derived from patients with a congenital disorder of glycosylation) that N-glycan remodeling is not required for the surface delivery of GluN3A-containing NMDARs.
29915530	3	76	from	role	510:513	arg1	NMDARs					555:560	GluN3A-containing NMDARs	537:560	GluN3A-containing NMDARs	537:560	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	3	77	theme	N-glycosylation	518:532	arg1	role					510:513	the role	506:513	the role of N-glycosylation in GluN3A-containing NMDARs	506:560	Previous studies showed that GluN1/GluN2 receptors are regulated by N-glycosylation; however, limited information is available regarding the role of N-glycosylation in GluN3A-containing NMDARs.
29915530	2	78	contain	have	275:278	arg2	properties					301:310	different functional properties	280:310	different functional properties	280:310	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	2	78	contain	have	275:278	arg1	NMDARs					222:227	NMDARs	222:227	NMDARs containing the nonconventional GluN3A subunit	222:273	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	0	79	theme	GluN3A-Containing	66:82	arg1	Receptors					89:97	GluN3A-Containing NMDA Receptors	66:97	GluN3A-Containing NMDA Receptors	66:97	N-Glycosylation Regulates the Trafficking and Surface Mobility of GluN3A-Containing NMDA Receptors.
29915530	2	80	theme	functional	290:299	arg1	properties					301:310	different functional properties	280:310	different functional properties	280:310	NMDARs containing the nonconventional GluN3A subunit have different functional properties compared to receptors comprised of GluN1/GluN2 subunits.
29915530	4	81	theme	GluN1	748:752	arg1	subunit					754:760	the GluN1 subunit	744:760	the GluN1 subunit	744:760	Using a combination of microscopy, biochemistry, and electrophysiology in mammalian cell lines and rat hippocampal neurons, we found that two asparagine residues (N203 and N368) in the GluN1 subunit and three asparagine residues (N145, N264 and N275) in the GluN3A subunit are required for surface delivery of GluN3A-containing NMDARs.
31125876	10	0	theme	adsorption/desorption	1286:1306	arg1	regeneration					1322:1333	adsorption/desorption and effective regeneration	1286:1333	adsorption/desorption and effective regeneration of the composite	1286:1350	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	1	1	theme	many	157:160	arg1	strategies					172:181	many treatment strategies	157:181	many treatment strategies	157:181	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	5	2	from	temperature	740:750	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	11	3	theme	enhanced	1357:1364	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	3	theme	enhanced	1357:1364	arg1	potential					1400:1408	excellent reuse potential	1384:1408	excellent reuse potential	1384:1408	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	3	theme	enhanced	1357:1364	arg1	granules					1423:1430	high stable granules	1411:1430	high stable granules	1411:1430	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	5	4	theme	scalable	676:683	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	11	5	theme	range	1532:1536	arg1	removal					1516:1522	the removal	1512:1522	the removal of wide range of heavy metals in water	1512:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	3	6	theme	processes	511:519	arg1	cycles					484:489	several cycles	476:489	several cycles of dehumidification processes	476:519	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	9	7	theme	nanocomposite	1148:1160	arg1	efficiency					1130:1139	the removal efficiency	1118:1139	the removal efficiency of the nanocomposite	1118:1160	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	11	8	from	water	1557:1561	arg1	range					1532:1536	wide range	1527:1536	wide range of heavy metals in water	1527:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	5	9	theme	one-pot	685:691	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	2	10	theme	sustainable	295:305	arg1	materials					317:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	10	theme	sustainable	295:305	arg1	factors					341:347	the prime factors	331:347	the prime factors for the success of such treatment systems in the field	331:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	11	theme	nanoscale	307:315	arg1	materials					317:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	11	theme	nanoscale	307:315	arg1	factors					341:347	the prime factors	331:347	the prime factors for the success of such treatment systems in the field	331:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	1	12	theme	treatment	162:170	arg1	strategies					172:181	many treatment strategies	157:181	many treatment strategies	157:181	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	11	13	theme	heavy	1541:1545	arg1	metals					1547:1552	heavy metals	1541:1552	heavy metals in water	1541:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	5	14	theme	sol-gel	693:699	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	3	15	theme	dehumidification	494:509	arg1	processes					511:519	dehumidification processes	494:519	dehumidification processes	494:519	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	2	16	theme	prime	335:339	arg1	materials					317:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	16	theme	prime	335:339	arg1	factors					341:347	the prime factors	331:347	the prime factors for the success of such treatment systems in the field	331:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	11	17	theme	wide	1527:1530	arg1	range					1532:1536	wide range	1527:1536	wide range of heavy metals in water	1527:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	2	18	theme	affordable	280:289	arg1	materials					317:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	18	theme	affordable	280:289	arg1	factors					341:347	the prime factors	331:347	the prime factors for the success of such treatment systems in the field	331:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	4	19	theme	chitosan	611:618	arg1	fibrils					620:626	chitosan fibrils	611:626	chitosan fibrils	611:626	The granulated composite desiccant is composed of boehmite nanoparticles reinforced with chitosan fibrils.
31125876	11	20	theme	excellent	1488:1496	arg1	candidate					1498:1506	an excellent candidate	1485:1506	an excellent candidate for the removal of wide range of heavy metals in water	1485:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	10	21	theme	regeneration	1322:1333	arg1	kinetics					1274:1281	the fast kinetics	1265:1281	the fast kinetics of adsorption/desorption and effective regeneration of the composite	1265:1350	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	2	22	theme	Developing	269:278	arg1	materials					317:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials	269:325	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	2	22	theme	Developing	269:278	arg1	factors					341:347	the prime factors	331:347	the prime factors for the success of such treatment systems in the field	331:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	11	23	theme	metals	1547:1552	arg1	range					1532:1536	wide range	1527:1536	wide range of heavy metals in water	1527:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	7	24	theme	desiccant	855:863	arg1	potential					832:840	The reuse potential	822:840	The reuse potential of exhausted desiccant towards enhanced removal of metal ions	822:902	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	10	25	theme	fast	1269:1272	arg1	kinetics					1274:1281	the fast kinetics	1265:1281	the fast kinetics of adsorption/desorption and effective regeneration of the composite	1265:1350	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	2	26	from	success	357:363	arg1	field					398:402	the field	394:402	the field	394:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	9	27	theme	fixed-bed	1051:1059	arg1	experiments					1079:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	10	28	theme	experimental	1234:1245	arg1	evidences					1247:1255	The experimental evidences	1230:1255	The experimental evidences	1230:1255	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	5	29	theme	simple	665:670	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	7	30	theme	metal	893:897	arg1	ions					899:902	metal ions	893:902	metal ions	893:902	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	2	31	theme	systems	383:389	arg1	success					357:363	the success	353:363	the success of such treatment systems in the field	353:402	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	9	32	theme	column	1061:1066	arg1	experiments					1079:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	7	33	theme	enhanced	873:880	arg1	removal					882:888	enhanced removal	873:888	enhanced removal of metal ions	873:902	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	7	34	theme	ions	899:902	arg1	removal					882:888	enhanced removal	873:888	enhanced removal of metal ions	873:902	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	9	35	theme	adsorption	1068:1077	arg1	experiments					1079:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	7	36	theme	exhausted	845:853	arg1	desiccant					855:863	exhausted desiccant	845:863	exhausted desiccant	845:863	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	5	37	theme	room	735:738	arg1	temperature					740:750	room temperature	735:750	room temperature	735:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	1	38	theme	widespread	105:114	arg1	occurrence					116:125	the widespread occurrence	101:125	the widespread occurrence	101:125	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	3	39	theme	present	409:415	arg1	study					417:421	The present study	405:421	The present study	405:421	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	4	40	theme	boehmite	572:579	arg1	nanoparticles					581:593	boehmite nanoparticles	572:593	boehmite nanoparticles reinforced with chitosan fibrils	572:626	The granulated composite desiccant is composed of boehmite nanoparticles reinforced with chitosan fibrils.
31125876	1	41	from	contaminants	246:257	arg1	water					262:266	water	262:266	water	262:266	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	9	42	theme	Batch	1041:1045	arg1	experiments					1079:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments	1041:1089	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	9	43	theme	adsorption	1210:1219	arg1	process					1221:1227	the adsorption process	1206:1227	the adsorption process	1206:1227	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	3	44	theme	desiccant	443:451	arg1	waste					453:457	desiccant waste	443:457	desiccant waste	443:457	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	4	45	theme	composite	537:545	arg1	desiccant					547:555	The granulated composite desiccant	522:555	The granulated composite desiccant	522:555	The granulated composite desiccant is composed of boehmite nanoparticles reinforced with chitosan fibrils.
31125876	11	46	theme	synthesis	1446:1454	arg1	approach					1456:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	3	47	theme	waste	453:457	arg1	use					436:438	the use	432:438	the use	432:438	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	4	48	theme	granulated	526:535	arg1	desiccant					547:555	The granulated composite desiccant	522:555	The granulated composite desiccant	522:555	The granulated composite desiccant is composed of boehmite nanoparticles reinforced with chitosan fibrils.
31125876	11	49	theme	stable	1416:1421	arg1	granules					1423:1430	high stable granules	1411:1430	high stable granules	1411:1430	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	49	theme	stable	1416:1421	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	10	50	theme	composite	1342:1350	arg1	regeneration					1322:1333	adsorption/desorption and effective regeneration	1286:1333	adsorption/desorption and effective regeneration of the composite	1286:1350	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	11	51	theme	excellent	1384:1392	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	51	theme	excellent	1384:1392	arg1	potential					1400:1408	excellent reuse potential	1384:1408	excellent reuse potential	1384:1408	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	52	theme	eco-friendly	1433:1444	arg1	approach					1456:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	6	53	theme	dehumidification/regeneration	784:812	arg1	cycles					814:819	dehumidification/regeneration cycles	784:819	dehumidification/regeneration cycles	784:819	The desiccant was employed for dehumidification/regeneration cycles.
31125876	11	54	theme	reuse	1394:1398	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	54	theme	reuse	1394:1398	arg1	potential					1400:1408	excellent reuse potential	1384:1408	excellent reuse potential	1384:1408	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	7	55	theme	reuse	826:830	arg1	potential					832:840	The reuse potential	822:840	The reuse potential of exhausted desiccant towards enhanced removal of metal ions	822:902	The reuse potential of exhausted desiccant towards enhanced removal of metal ions was analyzed and demonstrated.
31125876	5	56	theme	atmospheric	710:720	arg1	pressure					722:729	atmospheric pressure	710:729	atmospheric pressure	710:729	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	11	57	theme	removal	1366:1372	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	57	theme	removal	1366:1372	arg1	potential					1400:1408	excellent reuse potential	1384:1408	excellent reuse potential	1384:1408	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	57	theme	removal	1366:1372	arg1	granules					1423:1430	high stable granules	1411:1430	high stable granules	1411:1430	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	58	from	metals	1547:1552	arg1	water					1557:1561	water	1557:1561	water	1557:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	8	59	theme	chemical	1005:1012	arg1	composition					1014:1024	its chemical composition	1001:1024	its chemical composition	1001:1024	After adsorption the nanocomposite was characterized to establish its chemical composition and structure.
31125876	1	60	theme	potential	131:139	arg1	effects					148:154	potential health effects	131:154	potential health effects	131:154	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	10	61	theme	effective	1312:1320	arg1	regeneration					1322:1333	adsorption/desorption and effective regeneration	1286:1333	adsorption/desorption and effective regeneration of the composite	1286:1350	The experimental evidences confirm the fast kinetics of adsorption/desorption and effective regeneration of the composite.
31125876	11	62	theme	capacity	1374:1381	arg1	approach					1456:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach	1353:1463	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	63	from	range	1532:1536	arg1	water					1557:1561	water	1557:1561	water	1557:1561	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	1	64	theme	health	141:146	arg1	effects					148:154	potential health effects	131:154	potential health effects	131:154	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	9	65	theme	removal	1122:1128	arg1	efficiency					1130:1139	the removal efficiency	1118:1139	the removal efficiency of the nanocomposite	1118:1160	Batch and fixed-bed column adsorption experiments were performed to evaluate the removal efficiency of the nanocomposite and to assess the parameters that influence the adsorption process.
31125876	5	66	from	pressure	722:729	arg1	route					701:705	a simple and scalable one-pot sol-gel route	663:705	a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature	663:750	The composite was synthesized via a simple and scalable one-pot sol-gel route at atmospheric pressure and room temperature.
31125876	0	67	theme	metal	77:81	arg1	removal					83:89	heavy metal removal	71:89	heavy metal removal	71:89	Chitosan-boehmite desiccant composite as a promising adsorbent towards heavy metal removal.
31125876	11	68	theme	high	1411:1414	arg1	granules					1423:1430	high stable granules	1411:1430	high stable granules	1411:1430	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	11	68	theme	high	1411:1414	arg1	capacity					1374:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity	1353:1381	The enhanced removal capacity, excellent reuse potential, high stable granules, eco-friendly synthesis approach makes the adsorbent an excellent candidate for the removal of wide range of heavy metals in water.
31125876	2	69	theme	treatment	373:381	arg1	systems					383:389	such treatment systems	368:389	such treatment systems	368:389	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	3	70	theme	several	476:482	arg1	cycles					484:489	several cycles	476:489	several cycles of dehumidification processes	476:519	The present study explores the use of desiccant waste, exhausted after several cycles of dehumidification processes.
31125876	1	71	theme	heavy	234:238	arg1	contaminants					246:257	the heavy metal contaminants	230:257	the heavy metal contaminants in water	230:266	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31125876	0	72	theme	heavy	71:75	arg1	removal					83:89	heavy metal removal	71:89	heavy metal removal	71:89	Chitosan-boehmite desiccant composite as a promising adsorbent towards heavy metal removal.
31125876	2	73	theme	such	368:371	arg1	systems					383:389	such treatment systems	368:389	such treatment systems	368:389	Developing affordable and sustainable nanoscale materials are the prime factors for the success of such treatment systems in the field.
31125876	1	74	theme	metal	240:244	arg1	contaminants					246:257	the heavy metal contaminants	230:257	the heavy metal contaminants in water	230:266	Owing to the widespread occurrence and potential health effects, many treatment strategies have been developed across the world to remove the heavy metal contaminants in water.
31200223	7	0	theme	antibacterial	1146:1158	arg1	effect					1160:1165	an enhanced antibacterial effect	1134:1165	an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain	1134:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	2	1	from	form	375:378	arg1	ratio					345:349	the ratio	341:349	the ratio of the oxygen in the OH form	341:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	1	2	theme	antimicrobial	235:247	arg1	activity					249:256	antimicrobial activity	235:256	antimicrobial activity	235:256	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	1	2	theme	antimicrobial	235:247	arg1	sorbents					189:196	sorbents	189:196	sorbents for photodegradation	189:217	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	0	3	theme	dyes	81:84	arg1	removal					70:76	the removal	66:76	the removal of dyes	66:84	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes.
31200223	2	4	from	stability	316:324	arg1	samples					408:414	the samples	404:414	the samples	404:414	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	3	5	used	used	567:570	arg2	nanocomposite					500:512	the ZnO NPs/GG nanocomposite	485:512	the ZnO NPs/GG nanocomposite	485:512	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	2	6	from	oxygen	394:399	arg1	samples					408:414	the samples	404:414	the samples	404:414	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	8	7	theme	statistical	1333:1343	arg1	analysis					1345:1352	the statistical analysis	1329:1352	the statistical analysis using one-way ANOVA followed by Tukey's analysis	1329:1401	The obtained results were proved to be of high significance based on the statistical analysis using one-way ANOVA followed by Tukey's analysis.
31200223	8	8	theme	obtained	1264:1271	arg1	results					1273:1279	The obtained results	1260:1279	The obtained results	1260:1279	The obtained results were proved to be of high significance based on the statistical analysis using one-way ANOVA followed by Tukey's analysis.
31200223	6	9	theme	adsorption	1051:1060	arg1	model					1062:1066	the Freundlich adsorption model	1036:1066	the Freundlich adsorption model with pseudo-second-order kinetics	1036:1100	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
31200223	6	10	theme	pseudo-second-order	1073:1091	arg1	kinetics					1093:1100	pseudo-second-order kinetics	1073:1100	pseudo-second-order kinetics	1073:1100	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
31200223	7	11	theme	E-Coli/TOP10	1185:1196	arg1	strain					1210:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	5	12	theme	initial	892:898	arg1	concentration					904:916	the initial dye concentration	888:916	the initial dye concentration	888:916	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	1	13	theme	dye	262:264	arg1	removal					266:272	dye removal	262:272	dye removal	262:272	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	6	14	theme	Freundlich	1040:1049	arg1	model					1062:1066	the Freundlich adsorption model	1036:1066	the Freundlich adsorption model with pseudo-second-order kinetics	1036:1100	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
31200223	3	15	theme	tested	466:471	arg1	composites					473:482	all tested composites	462:482	all tested composites	462:482	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	5	16	theme	NPs/GG	775:780	arg1	nanocomposite					782:794	The ZnO NPs/GG nanocomposite	767:794	The ZnO NPs/GG nanocomposite	767:794	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	5	17	theme	concentration	904:916	arg1	percent					877:883	about 96 degradation percent	856:883	about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	856:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	5	17	theme	concentration	904:916	arg1	capacity					947:954	a high adsorption capacity	929:954	about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	856:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	5	18	dep	showed	796:801	arg1	reaching					847:854	reaching	847:854	showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	796:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	2	19	theme	oxygen	358:363	arg1	ratio					345:349	the ratio	341:349	the ratio of the oxygen in the OH form	341:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	0	20	theme	photoactive	7:17	arg1	nanoadsorbents					19:32	Robust photoactive nanoadsorbents	0:32	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes	0:84	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes.
31200223	1	21	theme	ZnO	87:89	arg1	nanostructures					91:104	ZnO nanostructures	87:104	ZnO nanostructures (NS)/guar gum (GG) nanocomposites	87:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	1	21	theme	ZnO	87:89	arg1	NS					107:108	NS	107:108	NS	107:108	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	7	22	theme	pure	1245:1248	arg1	gum					1255:1257	pure guar gum	1245:1257	pure guar gum	1245:1257	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	0	23	theme	Robust	0:5	arg1	nanoadsorbents					19:32	Robust photoactive nanoadsorbents	0:32	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes	0:84	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes.
31200223	3	24	theme	Rhodamine	685:693	arg1	RhB					698:700	RhB	698:700	RhB	698:700	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	24	theme	Rhodamine	685:693	arg1	B					695:695	the cationic dye Rhodamine B	668:695	the cationic dye Rhodamine B (RhB)	668:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	2	25	from	ratio	345:349	arg1	form					375:378	the OH form	368:378	the OH form	368:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	1	26	theme	nanostructures	91:104	arg1	gum					116:118	ZnO nanostructures (NS)/guar gum	87:118	ZnO nanostructures (NS)/guar gum (GG) nanocomposites	87:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	1	26	theme	nanostructures	91:104	arg1	GG					121:122	GG	121:122	GG	121:122	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	7	27	theme	guar	1250:1253	arg1	gum					1255:1257	pure guar gum	1245:1257	pure guar gum	1245:1257	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	2	28	theme	total	388:392	arg1	oxygen					394:399	the total oxygen	384:399	the total oxygen in the samples	384:414	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	3	29	dep	reactive	643:650	arg1	red					652:654	red	652:654	red	652:654	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	29	dep	reactive	643:650	arg1	RhB					698:700	RhB	698:700	RhB	698:700	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	29	dep	reactive	643:650	arg1	B					695:695	the cationic dye Rhodamine B	668:695	the cationic dye Rhodamine B (RhB)	668:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	29	dep	reactive	643:650	arg1	RR195					657:661	RR195	657:661	RR195	657:661	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	2	30	theme	ZnO	291:293	arg1	addition					279:286	The addition	275:286	The addition of ZnO	275:293	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	5	31	theme	high	931:934	arg1	capacity					947:954	a high adsorption capacity	929:954	about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	856:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	5	32	theme	degradation	865:875	arg1	percent					877:883	about 96 degradation percent	856:883	about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	856:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	3	33	theme	cationic	672:679	arg1	Rhodamine					685:693	the cationic dye Rhodamine	668:693	the cationic dye Rhodamine B (RhB)	668:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	5	34	theme	dye	900:902	arg1	concentration					904:916	the initial dye concentration	888:916	the initial dye concentration	888:916	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	8	35	theme	high	1302:1305	arg1	significance					1307:1318	high significance	1302:1318	high significance	1302:1318	The obtained results were proved to be of high significance based on the statistical analysis using one-way ANOVA followed by Tukey's analysis.
31200223	3	36	theme	NPs/GG	493:498	arg1	nanocomposite					500:512	the ZnO NPs/GG nanocomposite	485:512	the ZnO NPs/GG nanocomposite	485:512	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	37	theme	dye	681:683	arg1	Rhodamine					685:693	the cationic dye Rhodamine	668:693	the cationic dye Rhodamine B (RhB)	668:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	38	theme	degradation	599:609	arg1	studies					611:617	extended adsorption and degradation studies	575:617	extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB)	575:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	3	39	theme	photocatalytic	533:546	arg1	activity					548:555	the highest photocatalytic activity	521:555	the highest photocatalytic activity	521:555	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	2	40	theme	OH	372:373	arg1	form					375:378	the OH form	368:378	the OH form	368:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	0	41	theme	antibacterial	39:51	arg1	activity					53:60	antibacterial activity	39:60	antibacterial activity	39:60	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes.
31200223	6	42	with	model	1062:1066	arg1	kinetics					1093:1100	pseudo-second-order kinetics	1073:1100	pseudo-second-order kinetics	1073:1100	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
31200223	3	43	theme	extended	575:582	arg1	studies					611:617	extended adsorption and degradation studies	575:617	extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB)	575:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	5	44	theme	adsorption	936:945	arg1	capacity					947:954	a high adsorption capacity	929:954	about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1	856:973	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	5	45	theme	ZnO	771:773	arg1	nanocomposite					782:794	The ZnO NPs/GG nanocomposite	767:794	The ZnO NPs/GG nanocomposite	767:794	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	3	46	theme	adsorption	584:593	arg1	studies					611:617	extended adsorption and degradation studies	575:617	extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB)	575:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	0	47	with	nanoadsorbents	19:32	arg1	activity					53:60	antibacterial activity	39:60	antibacterial activity	39:60	Robust photoactive nanoadsorbents with antibacterial activity for the removal of dyes.
31200223	2	48	theme	GG	329:330	arg1	oxygen					394:399	the total oxygen	384:399	the total oxygen in the samples	384:414	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	2	48	theme	GG	329:330	arg1	stability					316:324	the thermal stability	304:324	the thermal stability of GG based on the ratio of the oxygen in the OH form	304:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	3	49	theme	reactive	643:650	arg1	dye					639:641	the anionic dye	627:641	the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB)	627:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	7	50	dep	showed	1127:1132	arg1	compared					1217:1224	compared	1217:1224	showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum	1127:1257	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	3	51	theme	highest	525:531	arg1	activity					548:555	the highest photocatalytic activity	521:555	the highest photocatalytic activity	521:555	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	7	52	theme	enhanced	1137:1144	arg1	effect					1160:1165	an enhanced antibacterial effect	1134:1165	an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain	1134:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	1	53	theme	/guar	110:114	arg1	gum					116:118	ZnO nanostructures (NS)/guar gum	87:118	ZnO nanostructures (NS)/guar gum (GG) nanocomposites	87:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	1	53	theme	/guar	110:114	arg1	GG					121:122	GG	121:122	GG	121:122	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	8	54	theme	one-way	1360:1366	arg1	ANOVA					1368:1372	one-way ANOVA	1360:1372	one-way ANOVA followed by Tukey's analysis	1360:1401	The obtained results were proved to be of high significance based on the statistical analysis using one-way ANOVA followed by Tukey's analysis.
31200223	7	55	theme	PTA	1199:1201	arg1	strain					1210:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	3	56	theme	anionic	631:637	arg1	dye					639:641	the anionic dye	627:641	the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB)	627:701	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	4	57	theme	adsorption	708:717	arg1	mechanism					719:727	The adsorption mechanism	704:727	The adsorption mechanism	704:727	The adsorption mechanism and kinetics were studied in details.
31200223	2	58	from	oxygen	358:363	arg1	form					375:378	the OH form	368:378	the OH form	368:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	1	59	theme	gum	116:118	arg1	sorbents					189:196	sorbents	189:196	sorbents for photodegradation	189:217	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	1	59	theme	gum	116:118	arg1	nanocomposites					125:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites	87:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites	87:138	ZnO nanostructures (NS)/guar gum (GG) nanocomposites have been successfully synthesized and tested as sorbents for photodegradation, adsorption and antimicrobial activity for dye removal.
31200223	3	60	theme	ZnO	489:491	arg1	nanocomposite					500:512	the ZnO NPs/GG nanocomposite	485:512	the ZnO NPs/GG nanocomposite	485:512	Among all tested composites, the ZnO NPs/GG nanocomposite showed the highest photocatalytic activity and hence used in extended adsorption and degradation studies against the anionic dye reactive red (RR195) and the cationic dye Rhodamine B (RhB).
31200223	2	61	theme	thermal	308:314	arg1	stability					316:324	the thermal stability	304:324	the thermal stability of GG based on the ratio of the oxygen in the OH form	304:378	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	7	62	theme	10989	1203:1207	arg1	strain					1210:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	E-Coli/TOP10 (PTA 10989) strain	1185:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	2	63	theme	FTIR	441:444	arg1	analyses					446:453	FTIR analyses	441:453	FTIR analyses	441:453	The addition of ZnO improves the thermal stability of GG based on the ratio of the oxygen in the OH form and the total oxygen in the samples as indicated via XPS and FTIR analyses.
31200223	7	64	theme	pristine	1229:1236	arg1	ZnO					1238:1240	pristine ZnO	1229:1240	pristine ZnO	1229:1240	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	5	65	theme	high	809:812	arg1	efficiency					822:831	quite high removal efficiency	803:831	quite high removal efficiency for both dyes	803:845	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	6	66	theme	dyes	999:1002	arg1	adsorption					980:989	The adsorption	976:989	The adsorption of both dyes on ZnO NPs/GG	976:1016	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
31200223	7	67	theme	ZnO/GG	1170:1175	arg1	effect					1160:1165	an enhanced antibacterial effect	1134:1165	an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain	1134:1215	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	5	68	theme	removal	814:820	arg1	efficiency					822:831	quite high removal efficiency	803:831	quite high removal efficiency for both dyes	803:845	The ZnO NPs/GG nanocomposite showed quite high removal efficiency for both dyes reaching about 96 degradation percent of the initial dye concentration as well as a high adsorption capacity reaching 70 mg g-1.
31200223	7	69	theme	antibacterial	1107:1119	arg1	assay					1121:1125	The antibacterial assay	1103:1125	The antibacterial assay	1103:1125	The antibacterial assay showed an enhanced antibacterial effect of ZnO/GG against E-Coli/TOP10 (PTA 10989) strain compared to pristine ZnO or pure guar gum.
31200223	6	70	from	adsorption	980:989	arg1	NPs/GG					1011:1016	NPs/GG	1011:1016	NPs/GG	1011:1016	The adsorption of both dyes on ZnO NPs/GG was found to obey the Freundlich adsorption model with pseudo-second-order kinetics.
32617037	8	0	theme	sugar	1210:1214	arg1	composition					1216:1226	its sugar composition	1206:1226	its sugar composition	1206:1226	The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis.
32617037	11	1	theme	Methylation	1616:1626	arg1	analysis					1628:1635	Methylation analysis	1616:1635	Methylation analysis	1616:1635	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	6	2	theme	sugar	887:891	arg1	composition					893:903	sugar composition	887:903	sugar composition	887:903	The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
32617037	12	3	theme	residues	2201:2208	arg1	positions					2184:2192	the C(O)4 positions	2174:2192	the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	2174:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	10	4	theme	β-glucosyl	1540:1549	arg1	reagent					1557:1563	the β-glucosyl Yariv reagent	1536:1563	the β-glucosyl Yariv reagent	1536:1563	RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan.
32617037	9	5	theme	96	1268:1269	arg1	kDa					1271:1273	kDa	1271:1273	kDa	1271:1273	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	3	6	theme	immunostimulatory	553:569	arg1	activity					571:578	the immunostimulatory activity	549:578	the immunostimulatory activity of red ginseng	549:593	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	5	7	theme	Immunostimulatary	718:734	arg1	activity					736:743	Immunostimulatary activity	718:743	Immunostimulatary activity of RGP-AP-I	718:755	Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity.
32617037	12	8	dep	CONCLUSION	1853:1862	arg1	assumed					1867:1873	assumed	1867:1873	assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	1867:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	9	from	positions	2184:2192	arg1	branch					2164:2169	branch	2164:2169	branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	2164:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	9	from	positions	2184:2192	arg1	chain					2222:2226	the main chain	2213:2226	the main chain of RGP-AP-I	2213:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	6	10	theme	RGP-AP-I	851:858	arg1	structure					838:846	The structure	834:846	The structure of RGP-AP-I	834:858	The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
32617037	2	11	theme	red	331:333	arg1	ginseng					335:341	red ginseng	331:341	red ginseng	331:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	1	12	theme	red	212:214	arg1	ginseng					216:222	red ginseng	212:222	red ginseng	212:222	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	12	13	with	chain	1980:1984	arg1	galactan					2120:2127	(1→4)-linked galactan	2107:2127	(1→4)-linked galactan	2107:2127	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	chains					2069:2074	side chains	2064:2074	side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan	2064:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	arabinan					2097:2104	(1→5)-linked arabinan	2084:2104	(1→5)-linked arabinan	2084:2104	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	unit					2011:2014	a repeating linkage unit	1991:2014	a repeating linkage unit	1991:2014	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	groups					2054:2059	three groups	2048:2059	three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan	2048:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	arabino-β-3,6-galactan					2134:2155	arabino-β-3,6-galactan	2134:2155	arabino-β-3,6-galactan	2134:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	13	with	chain	1980:1984	arg1	-GalAp-					2032:2038	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	3	14	theme	ginseng	587:593	arg1	activity					571:578	the immunostimulatory activity	549:578	the immunostimulatory activity of red ginseng	549:593	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	9	15	theme	Ara	1474:1476	arg1	%					1483:1483	Ara, 35.0%	1474:1483	%	1483:1483	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	15	theme	Ara	1474:1476	arg1	arabinose					1463:1471	arabinose	1463:1471	arabinose (Ara, 35.0%)	1463:1484	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	4	16	theme	size-exclusion	666:679	arg1	chromatography					681:694	size-exclusion chromatography	666:694	size-exclusion chromatography using Sephadex G-100	666:715	METHODS A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100.
32617037	10	17	theme	arabino-β-3,6-galactan	1592:1613	arg1	presence					1580:1587	the presence	1576:1587	the presence of arabino-β-3,6-galactan	1576:1613	RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan.
32617037	11	18	link	4-linked	1779:1786	arg1	GalAp					1788:1792	4-linked GalAp	1779:1792	4-linked GalAp	1779:1792	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	18	link	4-linked	1779:1786	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	7	19	theme	factor	1128:1133	arg1	-α					1140:1141	tumor necrosis factor (TNF)-α	1113:1141	tumor necrosis factor (TNF)-α	1113:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	9	20	theme	different	1317:1325	arg1	monosaccharides					1327:1341	nine different monosaccharides	1312:1341	nine different monosaccharides	1312:1341	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	1	21	theme	BACKGROUND	137:146	arg1	researchers					153:163	BACKGROUND Many researchers	137:163	BACKGROUND Many researchers	137:163	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	7	22	theme	tumor	1113:1117	arg1	TNF					1136:1138	TNF	1136:1138	TNF	1136:1138	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	7	22	theme	tumor	1113:1117	arg1	factor					1128:1133	tumor necrosis factor	1113:1133	tumor necrosis factor (TNF)-α	1113:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	2	23	theme	exact	266:270	arg1	characteristics					283:297	the exact structural characteristics	262:297	the exact structural characteristics of the acidic polysaccharide in red ginseng	262:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	11	24	link	2,4-linked	1758:1767	arg1	Rhap					1769:1772	2,4-linked Rhap	1758:1772	2,4-linked Rhap	1758:1772	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	24	link	2,4-linked	1758:1767	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	0	25	theme	rhamnogalacturonan	66:83	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.	0:135	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	7	26	theme	interleukin	1082:1092	arg1	IL					1095:1096	interleukin (IL)-6	1082:1099	interleukin (IL)-6	1082:1099	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	12	27	theme	linkage	2003:2009	arg1	unit					2011:2014	a repeating linkage unit	1991:2014	a repeating linkage unit	1991:2014	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	11	28	theme	5-linked	1743:1750	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	28	theme	5-linked	1743:1750	arg1	Araf					1752:1755	5-linked Araf	1743:1755	5-linked Araf	1743:1755	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	1	29	theme	various	183:189	arg1	due					228:230	due	228:230	due	228:230	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	1	29	theme	various	183:189	arg1	activities					198:207	the various immune activities	179:207	the various immune activities of red ginseng	179:222	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	2	30	from	ginseng	335:341	arg1	characteristics					283:297	the exact structural characteristics	262:297	the exact structural characteristics of the acidic polysaccharide in red ginseng	262:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	4	31	dep	METHODS	596:602	arg1	RGP-AP-I					622:629	RGP-AP-I	622:629	RGP-AP-I	622:629	METHODS A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100.
32617037	4	31	dep	METHODS	596:602	arg1	polysaccharide					606:619	A polysaccharide	604:619	METHODS A polysaccharide (RGP-AP-I)	596:630	METHODS A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100.
32617037	7	32	theme	various	1056:1062	arg1	IL					1095:1096	interleukin (IL)-6	1082:1099	interleukin (IL)-6	1082:1099	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	7	32	theme	various	1056:1062	arg1	cytokines					1064:1072	various cytokines	1056:1072	various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α	1056:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	7	32	theme	various	1056:1062	arg1	IL-12					1102:1106	IL-12	1102:1106	IL-12	1102:1106	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	7	32	theme	various	1056:1062	arg1	-α					1140:1141	tumor necrosis factor (TNF)-α	1113:1141	tumor necrosis factor (TNF)-α	1113:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	0	33	theme	red	124:126	arg1	ginseng					128:134	red ginseng	124:134	red ginseng	124:134	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	0	34	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.	0:135	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	11	35	link	3,6-linked	1726:1735	arg1	Galp					1737:1740	3,6-linked Galp	1726:1740	3,6-linked Galp	1726:1740	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	12	36	theme	-linked	2089:2095	arg1	arabinan					2097:2104	(1→5)-linked arabinan	2084:2104	(1→5)-linked arabinan	2084:2104	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	3	37	theme	red	429:431	arg1	ginseng					433:439	red ginseng	429:439	red ginseng	429:439	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	12	38	theme	chains	2069:2074	arg1	galactan					2120:2127	(1→4)-linked galactan	2107:2127	(1→4)-linked galactan	2107:2127	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	chains					2069:2074	side chains	2064:2074	side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan	2064:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	arabinan					2097:2104	(1→5)-linked arabinan	2084:2104	(1→5)-linked arabinan	2084:2104	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	unit					2011:2014	a repeating linkage unit	1991:2014	a repeating linkage unit	1991:2014	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	groups					2054:2059	three groups	2048:2059	three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan	2048:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	arabino-β-3,6-galactan					2134:2155	arabino-β-3,6-galactan	2134:2155	arabino-β-3,6-galactan	2134:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	38	theme	chains	2069:2074	arg1	-GalAp-					2032:2038	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	0	39	theme	red	32:34	arg1	rhamnogalacturonan					66:83	a red ginseng acidic polysaccharide rhamnogalacturonan I	30:85	a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng	30:134	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	8	40	theme	primary	1148:1154	arg1	structure					1156:1164	The primary structure	1144:1164	The primary structure of RGP-AP-I	1144:1176	The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis.
32617037	0	41	theme	acidic	44:49	arg1	rhamnogalacturonan					66:83	a red ginseng acidic polysaccharide rhamnogalacturonan I	30:85	a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng	30:134	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	6	42	theme	Yariv	917:921	arg1	reagent					923:929	β-glucosyl Yariv reagent	906:929	β-glucosyl Yariv reagent	906:929	The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
32617037	9	43	dep	rhamnose	1380:1387	arg1	%					1398:1398	9.5%	1395:1398	9.5%	1395:1398	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	43	dep	rhamnose	1380:1387	arg1	Rha					1390:1392	Rha	1390:1392	Rha	1390:1392	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	3	44	theme	moiety	497:502	arg1	property					474:481	the structural property	459:481	the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng	459:593	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	1	45	theme	acid	235:238	arg1	polysaccharides					240:254	acid polysaccharides	235:254	acid polysaccharides	235:254	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	12	46	theme	-Rhap-	2021:2026	arg1	-GalAp-					2032:2038	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	[→2)-Rhap-(1→4)-GalAp-(1→]	2017:2042	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	47	dep	-GalAp-	2032:2038	arg1	1→4					2028:2030	1→4	2028:2030	1→4	2028:2030	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	48	theme	main	2217:2220	arg1	chain					2222:2226	the main chain	2213:2226	the main chain of RGP-AP-I	2213:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	49	from	chain	2222:2226	arg1	positions					2184:2192	the C(O)4 positions	2174:2192	the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	2174:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	11	50	theme	rhamnogalacturonan	1824:1841	arg1	characteristics					1805:1819	characteristics	1805:1819	characteristics of rhamnogalacturonan I (RG-I)	1805:1850	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	50	theme	rhamnogalacturonan	1824:1841	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	12	51	theme	main	1975:1978	arg1	chain					1980:1984	a main chain	1973:1984	a main chain	1973:1984	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	10	52	theme	Yariv	1551:1555	arg1	reagent					1557:1563	the β-glucosyl Yariv reagent	1536:1563	the β-glucosyl Yariv reagent	1536:1563	RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan.
32617037	2	53	theme	acidic	306:311	arg1	polysaccharide					313:326	the acidic polysaccharide	302:326	the acidic polysaccharide in red ginseng	302:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	7	54	theme	RESULTS	957:963	arg1	macrophages					976:986	RESULTS Peritoneal macrophages	957:986	RESULTS Peritoneal macrophages stimulated using RGP-AP-I	957:1012	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	12	55	theme	RG-I	1941:1944	arg1	structure					1946:1954	the RG-I structure	1937:1954	the RG-I structure	1937:1954	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	10	56	with	reaction	1522:1529	arg1	reagent					1557:1563	the β-glucosyl Yariv reagent	1536:1563	the β-glucosyl Yariv reagent	1536:1563	RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan.
32617037	12	57	theme	Rha	2197:2199	arg1	residues					2201:2208	Rha residues	2197:2208	Rha residues in the main chain of RGP-AP-I	2197:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	11	58	theme	different	1674:1682	arg1	Rhap					1769:1772	2,4-linked Rhap	1758:1772	2,4-linked Rhap	1758:1772	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	58	theme	different	1674:1682	arg1	characteristics					1805:1819	characteristics	1805:1819	characteristics of rhamnogalacturonan I (RG-I)	1805:1850	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	58	theme	different	1674:1682	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	58	theme	different	1674:1682	arg1	Araf					1752:1755	5-linked Araf	1743:1755	5-linked Araf	1743:1755	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	58	theme	different	1674:1682	arg1	such					1703:1706	such	1703:1706	such	1703:1706	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	58	theme	different	1674:1682	arg1	GalAp					1788:1792	4-linked GalAp	1779:1792	4-linked GalAp	1779:1792	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	12	59	from	residues	2201:2208	arg1	chain					2222:2226	the main chain	2213:2226	the main chain of RGP-AP-I	2213:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	1	60	theme	immune	191:196	arg1	due					228:230	due	228:230	due	228:230	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	1	60	theme	immune	191:196	arg1	activities					198:207	the various immune activities	179:207	the various immune activities of red ginseng	179:222	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	8	61	theme	methylation	1232:1242	arg1	analysis					1244:1251	methylation analysis	1232:1251	methylation analysis	1232:1251	The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis.
32617037	9	62	theme	kDa	1271:1273	arg1	RGP-AP-I					1254:1261	RGP-AP-I	1254:1261	RGP-AP-I	1254:1261	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	62	theme	kDa	1271:1273	arg1	polysaccharide					1282:1295	a 96 kDa acidic polysaccharide	1266:1295	a 96 kDa acidic polysaccharide	1266:1295	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	4	63	theme	Sephadex	702:709	arg1	G-100					711:715	Sephadex G-100	702:715	Sephadex G-100	702:715	METHODS A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100.
32617037	9	64	theme	acidic	1275:1280	arg1	RGP-AP-I					1254:1261	RGP-AP-I	1254:1261	RGP-AP-I	1254:1261	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	64	theme	acidic	1275:1280	arg1	polysaccharide					1282:1295	a 96 kDa acidic polysaccharide	1266:1295	a 96 kDa acidic polysaccharide	1266:1295	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	5	65	theme	RGP-AP-I	748:755	arg1	activity					736:743	Immunostimulatary activity	718:743	Immunostimulatary activity of RGP-AP-I	718:755	Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity.
32617037	3	66	theme	red	583:585	arg1	ginseng					587:593	red ginseng	583:593	red ginseng	583:593	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	1	67	theme	ginseng	216:222	arg1	due					228:230	due	228:230	due	228:230	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	1	67	theme	ginseng	216:222	arg1	activities					198:207	the various immune activities	179:207	the various immune activities of red ginseng	179:222	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	12	68	theme	O	2180:2180	arg1	positions					2184:2192	the C(O)4 positions	2174:2192	the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	2174:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	69	dep	arabinan	2097:2104	arg1	1→5					2085:2087	1→5	2085:2087	1→5	2085:2087	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	4	70	theme	red	650:652	arg1	ginseng					654:660	red ginseng	650:660	red ginseng	650:660	METHODS A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100.
32617037	11	71	theme	2,4-linked	1758:1767	arg1	Rhap					1769:1772	2,4-linked Rhap	1758:1772	2,4-linked Rhap	1758:1772	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	71	theme	2,4-linked	1758:1767	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	7	72	theme	necrosis	1119:1126	arg1	TNF					1136:1138	TNF	1136:1138	TNF	1136:1138	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	7	72	theme	necrosis	1119:1126	arg1	factor					1128:1133	tumor necrosis factor	1113:1133	tumor necrosis factor (TNF)-α	1113:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	12	73	theme	RGP-AP-I	1914:1921	arg1	due					1930:1932	due	1930:1932	due	1930:1932	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	73	theme	RGP-AP-I	1914:1921	arg1	activity					1902:1909	the immunostimulatory activity	1880:1909	the immunostimulatory activity of RGP-AP-I	1880:1921	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	9	74	dep	galactose	1435:1443	arg1	Gal					1446:1448	Gal	1446:1448	Gal	1446:1448	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	74	dep	galactose	1435:1443	arg1	%					1455:1455	30.4%	1451:1455	30.4%	1451:1455	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	11	75	theme	4-linked	1779:1786	arg1	GalAp					1788:1792	4-linked GalAp	1779:1792	4-linked GalAp	1779:1792	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	75	theme	4-linked	1779:1786	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	2	76	theme	structural	272:281	arg1	characteristics					283:297	the exact structural characteristics	262:297	the exact structural characteristics of the acidic polysaccharide in red ginseng	262:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	1	77	theme	Many	148:151	arg1	researchers					153:163	BACKGROUND Many researchers	137:163	BACKGROUND Many researchers	137:163	BACKGROUND Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides.
32617037	2	78	from	characteristics	283:297	arg1	ginseng					335:341	red ginseng	331:341	red ginseng	331:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	12	79	theme	C	2178:2178	arg1	positions					2184:2192	the C(O)4 positions	2174:2192	the C(O)4 positions of Rha residues in the main chain of RGP-AP-I	2174:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	11	80	theme	3,6-linked	1726:1735	arg1	Galp					1737:1740	3,6-linked Galp	1726:1740	3,6-linked Galp	1726:1740	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	2	81	from	polysaccharide	313:326	arg1	ginseng					335:341	red ginseng	331:341	red ginseng	331:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	12	82	link	-linked	2089:2095	arg1	arabinan					2097:2104	(1→5)-linked arabinan	2084:2104	(1→5)-linked arabinan	2084:2104	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	83	theme	repeating	1993:2001	arg1	unit					2011:2014	a repeating linkage unit	1991:2014	a repeating linkage unit	1991:2014	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	3	84	from	ginseng	433:439	arg1	polysaccharide					409:422	the acidic polysaccharide	398:422	the acidic polysaccharide from red ginseng	398:439	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	12	85	theme	immunostimulatory	1884:1900	arg1	due					1930:1932	due	1930:1932	due	1930:1932	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	85	theme	immunostimulatory	1884:1900	arg1	activity					1902:1909	the immunostimulatory activity	1880:1909	the immunostimulatory activity of RGP-AP-I	1880:1921	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	2	86	theme	polysaccharide	313:326	arg1	characteristics					283:297	the exact structural characteristics	262:297	the exact structural characteristics of the acidic polysaccharide in red ginseng	262:341	But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated.
32617037	9	87	dep	acid	1415:1418	arg1	GalA					1421:1424	GalA	1421:1424	GalA	1421:1424	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	9	87	dep	acid	1415:1418	arg1	%					1431:1431	18.4%	1427:1431	18.4%	1427:1431	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	11	88	link	5-linked	1743:1750	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	88	link	5-linked	1743:1750	arg1	Araf					1752:1755	5-linked Araf	1743:1755	5-linked Araf	1743:1755	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	5	89	theme	macrophage	801:810	arg1	activity					824:831	macrophage stimulatory activity	801:831	macrophage stimulatory activity	801:831	Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity.
32617037	7	90	theme	cytokines	1064:1072	arg1	production					1042:1051	the production	1038:1051	the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α	1038:1141	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	3	91	theme	structural	463:472	arg1	property					474:481	the structural property	459:481	the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng	459:593	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	3	92	theme	acidic	402:407	arg1	polysaccharide					409:422	the acidic polysaccharide	398:422	the acidic polysaccharide from red ginseng	398:439	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	12	93	dep	galactan	2120:2127	arg1	1→4					2108:2110	1→4	2108:2110	1→4	2108:2110	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	3	94	theme	active	490:495	arg1	moiety					497:502	the active moiety	486:502	the active moiety	486:502	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	0	95	theme	ginseng	36:42	arg1	rhamnogalacturonan					66:83	a red ginseng acidic polysaccharide rhamnogalacturonan I	30:85	a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng	30:134	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	6	96	theme	methylation	935:945	arg1	analysis					947:954	methylation analysis	935:954	methylation analysis	935:954	The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
32617037	12	97	link	-linked	2112:2118	arg1	galactan					2120:2127	(1→4)-linked galactan	2107:2127	(1→4)-linked galactan	2107:2127	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	98	theme	RGP-AP-I	2231:2238	arg1	chain					2222:2226	the main chain	2213:2226	the main chain of RGP-AP-I	2213:2238	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	11	99	theme	glycosyl	1684:1691	arg1	Rhap					1769:1772	2,4-linked Rhap	1758:1772	2,4-linked Rhap	1758:1772	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	99	theme	glycosyl	1684:1691	arg1	characteristics					1805:1819	characteristics	1805:1819	characteristics of rhamnogalacturonan I (RG-I)	1805:1850	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	99	theme	glycosyl	1684:1691	arg1	linkages					1693:1700	21 different glycosyl linkages	1671:1700	21 different glycosyl linkages	1671:1700	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	99	theme	glycosyl	1684:1691	arg1	Araf					1752:1755	5-linked Araf	1743:1755	5-linked Araf	1743:1755	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	99	theme	glycosyl	1684:1691	arg1	such					1703:1706	such	1703:1706	such	1703:1706	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	11	99	theme	glycosyl	1684:1691	arg1	GalAp					1788:1792	4-linked GalAp	1779:1792	4-linked GalAp	1779:1792	Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
32617037	12	100	theme	side	2064:2067	arg1	galactan					2120:2127	(1→4)-linked galactan	2107:2127	(1→4)-linked galactan	2107:2127	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	100	theme	side	2064:2067	arg1	chains					2069:2074	side chains	2064:2074	side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan	2064:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	100	theme	side	2064:2067	arg1	arabinan					2097:2104	(1→5)-linked arabinan	2084:2104	(1→5)-linked arabinan	2084:2104	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	12	100	theme	side	2064:2067	arg1	arabino-β-3,6-galactan					2134:2155	arabino-β-3,6-galactan	2134:2155	arabino-β-3,6-galactan	2134:2155	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	5	101	theme	stimulatory	812:822	arg1	activity					824:831	macrophage stimulatory activity	801:831	macrophage stimulatory activity	801:831	Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity.
32617037	0	102	theme	polysaccharide	51:64	arg1	rhamnogalacturonan					66:83	a red ginseng acidic polysaccharide rhamnogalacturonan I	30:85	a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng	30:134	Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng.
32617037	9	103	theme	galacturonic	1402:1413	arg1	acid					1415:1418	galacturonic acid	1402:1418	galacturonic acid (GalA, 18.4%)	1402:1432	RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%).
32617037	8	104	theme	RGP-AP-I	1169:1176	arg1	structure					1156:1164	The primary structure	1144:1164	The primary structure of RGP-AP-I	1144:1176	The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis.
32617037	3	105	theme	polysaccharide	512:525	arg1	moiety					497:502	the active moiety	486:502	the active moiety	486:502	Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
32617037	6	106	theme	β-glucosyl	906:915	arg1	reagent					923:929	β-glucosyl Yariv reagent	906:929	β-glucosyl Yariv reagent	906:929	The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
32617037	12	107	theme	-linked	2112:2118	arg1	galactan					2120:2127	(1→4)-linked galactan	2107:2127	(1→4)-linked galactan	2107:2127	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
32617037	10	108	theme	considerable	1509:1520	arg1	reaction					1522:1529	an considerable reaction	1506:1529	an considerable reaction with the β-glucosyl Yariv reagent	1506:1563	RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan.
32617037	7	109	theme	Peritoneal	965:974	arg1	macrophages					976:986	RESULTS Peritoneal macrophages	957:986	RESULTS Peritoneal macrophages stimulated using RGP-AP-I	957:1012	RESULTS Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α.
32617037	12	110	dep	branch	2164:2169	arg1	chain					1980:1984	a main chain	1973:1984	a main chain	1973:1984	CONCLUSION we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
30171959	0	0	theme	release	110:116	arg1	pellets					118:124	extended release pellets	101:124	extended release pellets of zaltoprofen	101:139	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	1	1	theme	release	352:358	arg1	polymer					360:366	extended release polymer	343:366	extended release polymer	343:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	4	2	theme	keton	904:908	arg1	group					910:914	keton group	904:914	keton group	904:914	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	3	theme	composite	1139:1147	arg1	ratio					1075:1079	4:2 ratio	1071:1079	4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite	1071:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	4	theme	Sκ-C	373:376	arg1	composites					400:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	5	5	theme	Sκ-C	1112:1115	arg1	composite					1139:1147	Sκ-C cross-linked hydrogel composite	1112:1147	Sκ-C cross-linked hydrogel composite	1112:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	0	6	theme	extended	101:108	arg1	pellets					118:124	extended release pellets	101:124	extended release pellets of zaltoprofen	101:139	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	5	7	theme	drug	1165:1168	arg1	release					1170:1176	in-vitro drug release	1156:1176	in-vitro drug release up to 99.15 ± 2.20% up to 12h	1156:1206	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	8	theme	XRD	499:501	arg1	analysis					511:518	FTIR, DSC, XRD and SEM analysis	488:518	FTIR, DSC, XRD and SEM analysis	488:518	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	1	9	theme	extended	181:188	arg1	pellets					203:209	extended release (ER) pellets	181:209	extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer	181:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	5	10	theme	in-vivo	1271:1277	arg1	parameter					1279:1287	in-vivo parameter	1271:1287	in-vivo parameter	1271:1287	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	1	11	theme	cross-linked	267:278	arg1	composite					317:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	5	12	theme	viable	1490:1495	arg1	formulation					1416:1426	optimized formulation	1406:1426	optimized formulation (Sκ-C2) showed acceptable release pattern, hence	1406:1475	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	12	theme	viable	1490:1495	arg1	alternative					1497:1507	the viable alternative	1486:1507	the viable alternative	1486:1507	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	1	13	theme	starch-κ-carrageenan	280:299	arg1	composite					317:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	3	14	theme	in-vivo	734:740	arg1	parameter					758:766	in-vivo pharmacokinetic parameter	734:766	in-vivo pharmacokinetic parameter	734:766	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	14	theme	in-vivo	734:740	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	4	15	theme	group	910:914	arg1	formation					870:878	a formation	868:878	a formation of hemiacetal group and keton group of glyoxal	868:925	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	16	theme	acceptable	1443:1452	arg1	pattern					1462:1468	acceptable release pattern	1443:1468	acceptable release pattern	1443:1468	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	17	theme	ER	1512:1513	arg1	formulations					1520:1531	ER type formulations	1512:1531	ER type formulations	1512:1531	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	18	theme	94.00 ± 2.00	1229:1240	arg1	mucoadhesion					1213:1224	mucoadhesion	1213:1224	mucoadhesion of 94.00 ± 2.00 up to 12 h	1213:1251	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	19	theme	cross-linked	979:990	arg1	composite					1001:1009	Sκ-C cross-linked hydrogel composite	974:1009	Sκ-C cross-linked hydrogel composite	974:1009	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	3	20	theme	pharmacokinetic	742:756	arg1	parameter					758:766	in-vivo pharmacokinetic parameter	734:766	in-vivo pharmacokinetic parameter	734:766	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	20	theme	pharmacokinetic	742:756	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	1	21	link	cross-linked	267:278	arg1	composite					317:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	4	22	theme	cross-linked	801:812	arg1	composite					823:831	Sκ-C cross-linked hydrogel composite	796:831	Sκ-C cross-linked hydrogel composite	796:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	1	23	theme	release	190:196	arg1	pellets					203:209	extended release (ER) pellets	181:209	extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer	181:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	0	24	theme	zaltoprofen	129:139	arg1	pellets					118:124	extended release pellets	101:124	extended release pellets of zaltoprofen	101:139	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	5	25	theme	hydrogel	1130:1137	arg1	composite					1139:1147	Sκ-C cross-linked hydrogel composite	1112:1147	Sκ-C cross-linked hydrogel composite	1112:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	26	theme	DSC	494:496	arg1	analysis					511:518	FTIR, DSC, XRD and SEM analysis	488:518	FTIR, DSC, XRD and SEM analysis	488:518	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	5	27	theme	optimized	1406:1414	arg1	Sκ-C2					1429:1433	Sκ-C2	1429:1433	Sκ-C2	1429:1433	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	27	theme	optimized	1406:1414	arg1	formulation					1416:1426	optimized formulation	1406:1426	optimized formulation (Sκ-C2) showed acceptable release pattern, hence	1406:1475	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	27	theme	optimized	1406:1414	arg1	alternative					1497:1507	the viable alternative	1486:1507	the viable alternative	1486:1507	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	28	theme	Sκ-C	796:799	arg1	composite					823:831	Sκ-C cross-linked hydrogel composite	796:831	Sκ-C cross-linked hydrogel composite	796:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	29	theme	cross-linked	1117:1128	arg1	composite					1139:1147	Sκ-C cross-linked hydrogel composite	1112:1147	Sκ-C cross-linked hydrogel composite	1112:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	30	link	cross-linked	378:389	arg1	composites					400:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	4	31	theme	group	894:898	arg1	formation					870:878	a formation	868:878	a formation of hemiacetal group and keton group of glyoxal	868:925	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	0	32	link	cross-linked	65:76	arg1	composite					87:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	5	33	from	decrease	1301:1308	arg1	t1/2					1335:1338	t1/2	1335:1338	t1/2	1335:1338	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	33	from	decrease	1301:1308	arg1	max					1315:1317	C max	1313:1317	C max	1313:1317	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	34	link	cross-linked	1117:1128	arg1	composite					1139:1147	Sκ-C cross-linked hydrogel composite	1112:1147	Sκ-C cross-linked hydrogel composite	1112:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	1	35	theme	ER	199:200	arg1	pellets					203:209	extended release (ER) pellets	181:209	extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer	181:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	1	36	theme	Sκ-C	302:305	arg1	composite					317:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	4	37	theme	hemiacetal	883:892	arg1	group					894:898	hemiacetal group	883:898	hemiacetal group	883:898	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	38	theme	C	1313:1313	arg1	max					1315:1317	C max	1313:1317	C max	1313:1317	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	39	theme	release	1454:1460	arg1	pattern					1462:1468	acceptable release pattern	1443:1468	acceptable release pattern	1443:1468	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	3	40	theme	extrusion-spheronization	557:580	arg1	technique					582:590	extrusion-spheronization technique	557:590	extrusion-spheronization technique	557:590	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	5	41	dep	12h	1204:1206	arg1	up					1198:1199	up	1198:1199	up	1198:1199	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	42	theme	factorial	441:449	arg1	approach					458:465	a 32 full factorial design approach	431:465	a 32 full factorial design approach	431:465	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	0	43	theme	statistical	16:26	arg1	optimization					28:39	statistical optimization	16:39	statistical optimization	16:39	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	1	44	theme	hydrogel	308:315	arg1	composite					317:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite	267:325	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	5	45	theme	starch	1084:1089	arg1	ratio					1075:1079	4:2 ratio	1071:1079	4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite	1071:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	46	theme	FTIR	488:491	arg1	analysis					511:518	FTIR, DSC, XRD and SEM analysis	488:518	FTIR, DSC, XRD and SEM analysis	488:518	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	2	47	theme	full	436:439	arg1	approach					458:465	a 32 full factorial design approach	431:465	a 32 full factorial design approach	431:465	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	0	48	theme	starch-κ-carrageenan	44:63	arg1	composite					87:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	3	49	theme	optical	649:655	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	49	theme	optical	649:655	arg1	microscopy					657:666	optical microscopy	649:666	optical microscopy	649:666	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	5	50	theme	κ-carrageenan	1095:1107	arg1	ratio					1075:1079	4:2 ratio	1071:1079	4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite	1071:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	51	theme	significant	846:856	arg1	result					858:863	the significant result	842:863	the significant result	842:863	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	4	52	theme	Sκ-C	974:977	arg1	composite					1001:1009	Sκ-C cross-linked hydrogel composite	974:1009	Sκ-C cross-linked hydrogel composite	974:1009	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	0	53	theme	hydrogel	78:85	arg1	composite					87:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	5	54	theme	in-vitro	1156:1163	arg1	release					1170:1176	in-vitro drug release	1156:1176	in-vitro drug release up to 99.15 ± 2.20% up to 12h	1156:1206	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	55	link	cross-linked	979:990	arg1	composite					1001:1009	Sκ-C cross-linked hydrogel composite	974:1009	Sκ-C cross-linked hydrogel composite	974:1009	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	4	56	theme	hydrogel	992:999	arg1	composite					1001:1009	Sκ-C cross-linked hydrogel composite	974:1009	Sκ-C cross-linked hydrogel composite	974:1009	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	3	57	theme	production	610:619	arg1	FTIR					628:631	FTIR	628:631	FTIR	628:631	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	SEM					644:646	SEM	644:646	SEM	644:646	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	mucoadhesiveness					691:706	mucoadhesiveness	691:706	mucoadhesiveness	691:706	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	DSC					634:636	DSC	634:636	DSC	634:636	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	parameter					758:766	in-vivo pharmacokinetic parameter	734:766	in-vivo pharmacokinetic parameter	734:766	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	microscopy					657:666	optical microscopy	649:666	optical microscopy	649:666	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	XRD					639:641	XRD	639:641	XRD	639:641	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	dissolution					718:728	in-vitro dissolution	709:728	in-vitro dissolution	709:728	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	57	theme	production	610:619	arg1	characteristics					674:688	flow characteristics	669:688	flow characteristics	669:688	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	4	58	link	cross-linked	801:812	arg1	composite					823:831	Sκ-C cross-linked hydrogel composite	796:831	Sκ-C cross-linked hydrogel composite	796:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	0	59	theme	cross-linked	65:76	arg1	composite					87:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	starch-κ-carrageenan cross-linked hydrogel composite	44:95	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	4	60	theme	glyoxal	919:925	arg1	formation					870:878	a formation	868:878	a formation of hemiacetal group and keton group of glyoxal	868:925	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	3	61	theme	flow	669:672	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	61	theme	flow	669:672	arg1	characteristics					674:688	flow characteristics	669:688	flow characteristics	669:688	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	5	62	theme	4:2	1071:1073	arg1	ratio					1075:1079	4:2 ratio	1071:1079	4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite	1071:1147	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	1	63	theme	current	146:152	arg1	study					154:158	The current study	142:158	The current study	142:158	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	5	64	theme	type	1515:1518	arg1	formulations					1520:1531	ER type formulations	1512:1531	ER type formulations	1512:1531	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	1	65	contain	containing	224:233	arg2	zaltoprofen					235:245	zaltoprofen	235:245	zaltoprofen	235:245	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	1	65	contain	containing	224:233	arg2	drug					258:261	a model drug	250:261	a model drug	250:261	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	1	65	contain	containing	224:233	arg1	pellets					203:209	extended release (ER) pellets	181:209	extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer	181:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	1	66	dep	containing	224:233	arg1	formulations					211:222	formulations	211:222	formulations	211:222	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	5	67	from	increase	1323:1330	arg1	t1/2					1335:1338	t1/2	1335:1338	t1/2	1335:1338	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	67	from	increase	1323:1330	arg1	max					1315:1317	C max	1313:1317	C max	1313:1317	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	68	theme	FTIR	773:776	arg1	interpretation					778:791	The FTIR interpretation	769:791	The FTIR interpretation of Sκ-C cross-linked hydrogel composite	769:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	69	dep	%	1196:1196	arg1	up					1178:1179	up	1178:1179	up	1178:1179	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	70	dep	12 h	1248:1251	arg1	up					1242:1243	up	1242:1243	up	1242:1243	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	4	71	theme	hydrogel	814:821	arg1	composite					823:831	Sκ-C cross-linked hydrogel composite	796:831	Sκ-C cross-linked hydrogel composite	796:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	5	72	theme	drug	1358:1361	arg1	>12 h.					1371:1376	drug release >12 h.	1358:1376	drug release >12 h.	1358:1376	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	3	73	theme	matrix	525:530	arg1	pellets					532:538	The matrix pellets	521:538	The matrix pellets	521:538	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	4	74	theme	composite	823:831	arg1	interpretation					778:791	The FTIR interpretation	769:791	The FTIR interpretation of Sκ-C cross-linked hydrogel composite	769:831	The FTIR interpretation of Sκ-C cross-linked hydrogel composite provides the significant result as a formation of hemiacetal group and keton group of glyoxal is abolished; hence it could be satisfied that Sκ-C cross-linked hydrogel composite was formed.
30171959	2	75	theme	SEM	507:509	arg1	analysis					511:518	FTIR, DSC, XRD and SEM analysis	488:518	FTIR, DSC, XRD and SEM analysis	488:518	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	5	76	theme	optimized	1027:1035	arg1	Sκ-C2					1050:1054	Sκ-C2	1050:1054	Sκ-C2	1050:1054	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	76	theme	optimized	1027:1035	arg1	formulation					1037:1047	The optimized formulation	1023:1047	The optimized formulation (Sκ-C2)	1023:1055	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	5	77	theme	release	1363:1369	arg1	>12 h.					1371:1376	drug release >12 h.	1358:1376	drug release >12 h.	1358:1376	The optimized formulation (Sκ-C2) was contained 4:2 ratio of starch and κ-carrageenan of Sκ-C cross-linked hydrogel composite showed in-vitro drug release up to 99.15 ± 2.20% up to 12h, and mucoadhesion of 94.00 ± 2.00 up to 12 h, respectively and in-vivo parameter were showed decrease in C max and increase in t1/2 significantly and drug release >12 h. Hence it was concluded that optimized formulation (Sκ-C2) showed acceptable release pattern, hence would be the viable alternative to ER type formulations.
30171959	2	78	theme	hydrogel	391:398	arg1	composites					400:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	1	79	theme	model	252:256	arg1	drug					258:261	a model drug	250:261	a model drug	250:261	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	1	79	theme	model	252:256	arg1	zaltoprofen					235:245	zaltoprofen	235:245	zaltoprofen	235:245	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	0	80	theme	composite	87:95	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	0	80	theme	composite	87:95	arg1	optimization					28:39	statistical optimization	16:39	statistical optimization	16:39	Fabrication and statistical optimization of starch-κ-carrageenan cross-linked hydrogel composite for extended release pellets of zaltoprofen.
30171959	1	81	theme	extended	343:350	arg1	polymer					360:366	extended release polymer	343:366	extended release polymer	343:366	The current study was aimed to develop extended release (ER) pellets formulations containing zaltoprofen as a model drug and cross-linked starch-κ-carrageenan (Sκ-C) hydrogel composite as a binder and extended release polymer.
30171959	2	82	theme	design	451:456	arg1	approach					458:465	a 32 full factorial design approach	431:465	a 32 full factorial design approach	431:465	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30171959	3	83	theme	in-vitro	709:716	arg1	yield					621:625	production yield	610:625	production yield	610:625	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	3	83	theme	in-vitro	709:716	arg1	dissolution					718:728	in-vitro dissolution	709:728	in-vitro dissolution	709:728	The matrix pellets were prepared by extrusion-spheronization technique and characterized production yield, FTIR, DSC, XRD, SEM, optical microscopy, flow characteristics, mucoadhesiveness, in-vitro dissolution and in-vivo pharmacokinetic parameter.
30171959	2	84	theme	cross-linked	378:389	arg1	composites					400:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites	369:409	The Sκ-C cross-linked hydrogel composites were prepared using a 32 full factorial design approach and characterized by FTIR, DSC, XRD and SEM analysis.
30120971	1	0	theme	xanthan	292:298	arg1	gum					300:302	xanthan gum	292:302	xanthan gum (XG)	292:307	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	0	theme	xanthan	292:298	arg1	XG					305:306	XG	305:306	XG	305:306	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	2	1	theme	whey	724:727	arg1	proteins					729:736	the microparticulated whey proteins	702:736	the microparticulated whey proteins	702:736	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	0	2	theme	oxidative	63:71	arg1	stability					73:81	the oxidative stability	59:81	the oxidative stability of O/W emulsions	59:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	2	3	theme	fluorescence	594:605	arg1	spectroscopy					607:618	steady-state fluorescence spectroscopy	581:618	steady-state fluorescence spectroscopy	581:618	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	4	theme	emulsions	375:383	arg1	stability					358:366	the lipid oxidative stability	338:366	the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures	338:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	5	dep	protein	201:207	arg1	concentrate					209:219	concentrate	209:219	concentrate	209:219	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	2	6	theme	size	478:481	arg1	chromatography					493:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	0	7	theme	O/W	86:88	arg1	emulsions					90:98	O/W emulsions	86:98	O/W emulsions	86:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	0	8	from	Influence	0:8	arg1	stability					73:81	the oxidative stability	59:81	the oxidative stability of O/W emulsions	59:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	2	9	theme	steady-state	581:592	arg1	spectroscopy					607:618	steady-state fluorescence spectroscopy	581:618	steady-state fluorescence spectroscopy	581:618	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	10	with	mixtures	278:285	arg1	gum					300:302	xanthan gum	292:302	xanthan gum (XG)	292:307	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	10	with	mixtures	278:285	arg1	XG					305:306	XG	305:306	XG	305:306	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	5	11	theme	peroxide	1000:1007	arg1	PVs					1017:1019	PVs	1017:1019	PVs	1017:1019	In addition, the peroxide values (PVs) were measured to evaluate the oxidative stability of each emulsion.
30120971	5	11	theme	peroxide	1000:1007	arg1	values					1009:1014	the peroxide values	996:1014	the peroxide values (PVs)	996:1020	In addition, the peroxide values (PVs) were measured to evaluate the oxidative stability of each emulsion.
30120971	8	12	contain	containing	1499:1508	arg1	structures					1488:1497	novel structures	1482:1497	novel structures containing lipid phases that are susceptible to oxidation	1482:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	8	12	contain	containing	1499:1508	arg2	phases					1516:1521	lipid phases	1510:1521	lipid phases that are susceptible to oxidation	1510:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	2	13	dep	Fourier	509:515	arg1	transform					517:525	transform	517:525	transform infrared spectrometry	517:547	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	14	theme	microparticulated	231:247	arg1	MWP					263:265	MWP	263:265	MWP	263:265	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	14	theme	microparticulated	231:247	arg1	protein					254:260	microparticulated whey protein	231:260	microparticulated whey protein (MWP)	231:266	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	2	15	theme	exclusion	483:491	arg1	chromatography					493:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	7	16	theme	complex	1274:1280	arg1	layer					1282:1286	a single complex layer	1265:1286	a single complex layer formed by the spherical protein microparticles and XG	1265:1340	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	1	17	theme	same	394:397	arg1	composition					411:421	the same interfacial composition	390:421	the same interfacial composition	390:421	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	7	18	theme	spherical	1302:1310	arg1	microparticles					1320:1333	the spherical protein microparticles	1298:1333	the spherical protein microparticles	1298:1333	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	1	19	theme	oil-in-water	145:156	arg1	emulsions					164:172	oil-in-water (O/W) emulsions	145:172	oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP)	145:266	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	0	20	theme	emulsions	90:98	arg1	stability					73:81	the oxidative stability	59:81	the oxidative stability of O/W emulsions	59:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	8	21	contain	has	1430:1432	arg1	study					1424:1428	This study	1419:1428	This study	1419:1428	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	8	21	contain	has	1430:1432	arg2	implications					1446:1457	significant implications	1434:1457	significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation	1434:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	6	22	theme	-XG-stabilized	1124:1137	arg1	emulsions					1139:1147	MWP(pH 6.5)-XG-stabilized emulsions	1113:1147	MWP(pH 6.5)-XG-stabilized emulsions	1113:1147	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	1	23	theme	interfacial	399:409	arg1	composition					411:421	the same interfacial composition	390:421	the same interfacial composition	390:421	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	7	24	theme	protein	1312:1318	arg1	microparticles					1320:1333	the spherical protein microparticles	1298:1333	the spherical protein microparticles	1298:1333	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	4	25	theme	oil	885:887	arg1	Emulsions					861:869	Emulsions	861:869	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures	861:952	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures were subjected to oxidation.
30120971	5	26	theme	emulsion	1080:1087	arg1	stability					1062:1070	the oxidative stability	1048:1070	the oxidative stability of each emulsion	1048:1087	In addition, the peroxide values (PVs) were measured to evaluate the oxidative stability of each emulsion.
30120971	2	27	theme	natural	677:683	arg1	protein					690:696	natural whey protein	677:696	natural whey protein	677:696	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	4	28	theme	%	876:876	arg1	oil					885:887	60% peanut oil	874:887	60% peanut oil	874:887	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures were subjected to oxidation.
30120971	1	29	theme	O/W	159:161	arg1	emulsions					164:172	oil-in-water (O/W) emulsions	145:172	oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP)	145:266	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	4	30	theme	protein-XG	934:943	arg1	mixtures					945:952	the protein-XG mixtures	930:952	the protein-XG mixtures	930:952	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures were subjected to oxidation.
30120971	1	31	theme	whey	249:252	arg1	MWP					263:265	MWP	263:265	MWP	263:265	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	31	theme	whey	249:252	arg1	protein					254:260	microparticulated whey protein	231:260	microparticulated whey protein (MWP)	231:266	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	7	32	theme	emulsions	1388:1396	arg1	oxidation					1371:1379	the lipid oxidation	1361:1379	the lipid oxidation of O/W emulsions than a double layer	1361:1416	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	6	33	theme	MWP	1113:1115	arg1	emulsions					1139:1147	MWP(pH 6.5)-XG-stabilized emulsions	1113:1147	MWP(pH 6.5)-XG-stabilized emulsions	1113:1147	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	8	34	theme	novel	1482:1486	arg1	structures					1488:1497	novel structures	1482:1497	novel structures containing lipid phases that are susceptible to oxidation	1482:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	6	35	theme	oxidative	1161:1169	arg1	stabilities					1171:1181	high oxidative stabilities	1156:1181	high oxidative stabilities that were not significantly different from each other	1156:1235	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	0	36	theme	protein	21:27	arg1	structures					29:38	initial protein structures	13:38	initial protein structures	13:38	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	2	37	used	used	625:628	arg2	analysis					568:575	X-ray diffraction analysis	550:575	X-ray diffraction analysis	550:575	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	2	37	used	used	625:628	arg2	Fourier					509:515	Fourier	509:515	Fourier transform infrared spectrometry	509:547	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	2	37	used	used	625:628	arg2	chromatography					493:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	2	37	used	used	625:628	arg2	spectroscopy					607:618	steady-state fluorescence spectroscopy	581:618	steady-state fluorescence spectroscopy	581:618	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	0	38	theme	whey	114:117	arg1	protein					119:125	whey protein	114:125	whey protein	114:125	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	1	39	theme	different	427:435	arg1	structures					449:458	different interfacial structures	427:458	different interfacial structures	427:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	0	40	theme	initial	13:19	arg1	structures					29:38	initial protein structures	13:38	initial protein structures	13:38	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	6	41	from	other	1231:1235	arg1	different					1211:1219	different	1211:1219	different	1211:1219	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	8	42	theme	significant	1434:1444	arg1	implications					1446:1457	significant implications	1434:1457	significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation	1434:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	1	43	with	emulsions	164:172	arg1	gum					300:302	xanthan gum	292:302	xanthan gum (XG)	292:307	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	43	with	emulsions	164:172	arg1	XG					305:306	XG	305:306	XG	305:306	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	7	44	theme	single	1267:1272	arg1	layer					1282:1286	a single complex layer	1265:1286	a single complex layer formed by the spherical protein microparticles and XG	1265:1340	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	0	45	theme	structures	29:38	arg1	Influence					0:8	Influence	0:8	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions	0:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	7	46	theme	double	1405:1410	arg1	layer					1412:1416	a double layer	1403:1416	a double layer	1403:1416	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	2	47	dep	reveal	633:638	arg1	MWP					739:741	MWP	739:741	MWP	739:741	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	48	theme	interfacial	437:447	arg1	structures					449:458	different interfacial structures	427:458	different interfacial structures	427:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	49	theme	natural	188:194	arg1	protein					201:207	natural whey protein concentrate	188:219	natural whey protein concentrate (WPC)	188:225	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	49	theme	natural	188:194	arg1	WPC					222:224	WPC	222:224	WPC	222:224	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	0	50	theme	gum	52:54	arg1	Influence					0:8	Influence	0:8	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions	0:98	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	2	51	dep	transform	517:525	arg1	infrared					527:534	infrared	527:534	transform infrared spectrometry	517:547	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	52	theme	lipid	342:346	arg1	stability					358:366	the lipid oxidative stability	338:366	the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures	338:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	53	theme	structures	449:458	arg1	stability					358:366	the lipid oxidative stability	338:366	the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures	338:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	2	54	from	differences	644:654	arg1	structures					663:672	the structures	659:672	the structures of natural whey protein and the microparticulated whey proteins	659:736	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	0	55	theme	xanthan	44:50	arg1	gum					52:54	xanthan gum	44:54	xanthan gum	44:54	Influence of initial protein structures and xanthan gum on the oxidative stability of O/W emulsions stabilized by whey protein.
30120971	1	56	with	emulsions	375:383	arg1	composition					411:421	the same interfacial composition	390:421	the same interfacial composition	390:421	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	2	57	theme	diffraction	556:566	arg1	analysis					568:575	X-ray diffraction analysis	550:575	X-ray diffraction analysis	550:575	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	58	theme	oxidative	348:356	arg1	stability					358:366	the lipid oxidative stability	338:366	the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures	338:458	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	8	59	theme	structures	1488:1497	arg1	development					1467:1477	the development	1463:1477	the development of novel structures containing lipid phases that are susceptible to oxidation	1463:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	6	60	theme	high	1156:1159	arg1	stabilities					1171:1181	high oxidative stabilities	1156:1181	high oxidative stabilities that were not significantly different from each other	1156:1235	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	3	61	theme	w/w	807:809	arg1	XG					799:800	XG	799:800	XG	799:800	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
30120971	3	61	theme	w/w	807:809	arg1	%					805:805	30% w/w	803:809	30% w/w	803:809	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
30120971	2	62	theme	X-ray	550:554	arg1	analysis					568:575	X-ray diffraction analysis	550:575	X-ray diffraction analysis	550:575	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	2	63	theme	whey	685:688	arg1	protein					690:696	natural whey protein	677:696	natural whey protein	677:696	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	2	64	theme	protein	690:696	arg1	structures					663:672	the structures	659:672	the structures of natural whey protein and the microparticulated whey proteins	659:736	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	7	65	theme	lipid	1365:1369	arg1	oxidation					1371:1379	the lipid oxidation	1361:1379	the lipid oxidation of O/W emulsions than a double layer	1361:1416	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	1	66	theme	whey	196:199	arg1	protein					201:207	natural whey protein concentrate	188:219	natural whey protein concentrate (WPC)	188:225	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	1	66	theme	whey	196:199	arg1	WPC					222:224	WPC	222:224	WPC	222:224	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	7	67	theme	O/W	1384:1386	arg1	emulsions					1388:1396	O/W emulsions	1384:1396	O/W emulsions	1384:1396	The results indicated that a single complex layer formed by the spherical protein microparticles and XG can better inhibit the lipid oxidation of O/W emulsions than a double layer.
30120971	2	68	theme	microparticulated	706:722	arg1	proteins					729:736	the microparticulated whey proteins	702:736	the microparticulated whey proteins	702:736	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	4	69	theme	60	874:875	arg1	%					876:876	%	876:876	%	876:876	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures were subjected to oxidation.
30120971	3	70	theme	w/w	790:792	arg1	%					788:788	70% w/w	786:792	70% w/w	786:792	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
30120971	3	70	theme	w/w	790:792	arg1	Dispersions					757:767	Dispersions	757:767	Dispersions of the proteins (70% w/w)	757:793	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
30120971	2	71	theme	High-performance	461:476	arg1	chromatography					493:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography	461:506	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	1	72	theme	O/W	371:373	arg1	emulsions					375:383	O/W emulsions	371:383	O/W emulsions with the same interfacial composition	371:421	In this article, oil-in-water (O/W) emulsions stabilized by natural whey protein concentrate (WPC) and microparticulated whey protein (MWP) and their mixtures with xanthan gum (XG) were prepared to investigate the lipid oxidative stability of O/W emulsions with the same interfacial composition but different interfacial structures.
30120971	5	73	theme	each	1075:1078	arg1	emulsion					1080:1087	each emulsion	1075:1087	each emulsion	1075:1087	In addition, the peroxide values (PVs) were measured to evaluate the oxidative stability of each emulsion.
30120971	8	74	theme	lipid	1510:1514	arg1	phases					1516:1521	lipid phases	1510:1521	lipid phases that are susceptible to oxidation	1510:1555	This study has significant implications for the development of novel structures containing lipid phases that are susceptible to oxidation.
30120971	5	75	theme	oxidative	1052:1060	arg1	stability					1062:1070	the oxidative stability	1048:1070	the oxidative stability of each emulsion	1048:1087	In addition, the peroxide values (PVs) were measured to evaluate the oxidative stability of each emulsion.
30120971	6	76	theme	MWP	1094:1096	arg1	-XG					1105:1107	The MWP(pH 4.5)-XG	1090:1107	The MWP(pH 4.5)-XG	1090:1107	The MWP(pH 4.5)-XG and MWP(pH 6.5)-XG-stabilized emulsions showed high oxidative stabilities that were not significantly different from each other.
30120971	2	77	theme	proteins	729:736	arg1	structures					663:672	the structures	659:672	the structures of natural whey protein and the microparticulated whey proteins	659:736	High-performance size exclusion chromatography, Fourier transform infrared spectrometry, X-ray diffraction analysis and steady-state fluorescence spectroscopy were used to reveal the differences in the structures of natural whey protein and the microparticulated whey proteins (MWP, pH 3.5-8.5).
30120971	4	78	theme	peanut	878:883	arg1	oil					885:887	60% peanut oil	874:887	60% peanut oil	874:887	Emulsions of 60% peanut oil that were stabilized by the proteins and the protein-XG mixtures were subjected to oxidation.
30120971	3	79	theme	proteins	776:783	arg1	%					788:788	70% w/w	786:792	70% w/w	786:792	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
30120971	3	79	theme	proteins	776:783	arg1	Dispersions					757:767	Dispersions	757:767	Dispersions of the proteins (70% w/w)	757:793	Dispersions of the proteins (70% w/w) and XG (30% w/w) were mixed to prepare the mixtures (protein-XG).
29800297	2	0	theme	sulfate	498:504	arg1	terms					477:481	terms	477:481	terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition	477:577	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	1	theme	heparan	732:738	arg1	sulfate					740:746	heparan sulfate	732:746	heparan sulfate	732:746	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	2	2	theme	xyloside	375:382	arg1	concentration					345:357	the xyloside concentration	332:357	the xyloside concentration	332:357	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	2	2	theme	xyloside	375:382	arg1	type					367:370	the type	363:370	the type of xyloside	363:382	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	2	2	theme	xyloside	375:382	arg1	type					405:408	the cell type	396:408	the cell type	396:408	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	4	3	theme	nonsulfated	1116:1126	arg1	disaccharides					1128:1140	6S-sulfated and/or nonsulfated disaccharides	1097:1140	6S-sulfated and/or nonsulfated disaccharides	1097:1140	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	2	4	theme	heparan	490:496	arg1	sulfate					498:504	heparan sulfate	490:504	heparan sulfate	490:504	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	4	5	theme	xyloside	922:929	arg1	type					914:917	type	914:917	type	914:917	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	5	theme	xyloside	922:929	arg1	concentration					896:908	the xyloside concentration	883:908	the xyloside concentration	883:908	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	6	theme	increasing	1347:1356	arg1	concentrations					1358:1371	increasing concentrations	1347:1371	increasing concentrations of xyloside	1347:1383	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	2	7	theme	disaccharide	554:565	arg1	composition					567:577	disaccharide composition	554:577	disaccharide composition	554:577	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	4	8	theme	higher	936:941	arg1	extent					943:948	a higher extent	934:948	a higher extent than that of the heparan sulfate	934:981	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	5	9	theme	different	1560:1568	arg1	types					1570:1574	different types	1560:1574	different types	1560:1574	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	4	10	theme	disaccharide	804:815	arg1	composition					817:827	The disaccharide composition	800:827	The disaccharide composition of the chondroitin/dermatan sulfate	800:863	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	11	from	proportion	988:997	arg1	sulfate					1056:1062	the chondroitin/dermatan sulfate	1031:1062	the chondroitin/dermatan sulfate	1031:1062	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	3	12	theme	greatest	603:610	arg1	influence					612:620	greatest influence	603:620	greatest influence	603:620	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	5	13	theme	large	1447:1451	arg1	glycosaminoglycans					1464:1481	glycosaminoglycans	1464:1481	glycosaminoglycans in living cells	1464:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	5	13	theme	large	1447:1451	arg1	amounts					1453:1459	large amounts	1447:1459	large amounts of glycosaminoglycans in living cells	1447:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	4	14	theme	4S-sulfated	1002:1012	arg1	disaccharides					1014:1026	4S-sulfated disaccharides	1002:1026	4S-sulfated disaccharides	1002:1026	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	5	15	theme	types	1570:1574	arg1	xylosides					1547:1555	xylosides	1547:1555	xylosides of different types and at different concentrations	1547:1606	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	4	16	theme	xyloside	1220:1227	arg1	hydrophobicity					1229:1242	xyloside hydrophobicity	1220:1242	xyloside hydrophobicity	1220:1242	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	1	17	theme	living	189:194	arg1	cells					196:200	living cells	189:200	living cells	189:200	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	2	18	theme	sulfate	531:537	arg1	terms					477:481	terms	477:481	terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition	477:577	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	19	theme	sulfate	740:746	arg1	proportion					718:727	the proportion	714:727	the proportion of heparan sulfate	714:746	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	3	19	theme	sulfate	740:746	arg1	complexity					756:765	the complexity	752:765	the complexity of chondroitin/dermatan sulfate	752:797	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	5	20	from	cells	1493:1497	arg1	glycosaminoglycans					1464:1481	glycosaminoglycans	1464:1481	glycosaminoglycans in living cells	1464:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	5	20	from	cells	1493:1497	arg1	amounts					1453:1459	large amounts	1447:1459	large amounts of glycosaminoglycans in living cells	1447:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	2	21	theme	glycosaminoglycans	455:472	arg1	structure					426:434	the structure	422:434	the structure of xyloside-primed glycosaminoglycans	422:472	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	5	22	theme	different	1583:1591	arg1	concentrations					1593:1606	different concentrations	1583:1606	different concentrations	1583:1606	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	2	23	theme	xyloside	336:343	arg1	concentration					345:357	the xyloside concentration	332:357	the xyloside concentration	332:357	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	5	24	from	glycosaminoglycans	1464:1481	arg1	cells					1493:1497	living cells	1486:1497	living cells	1486:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	2	25	theme	xyloside-primed	439:453	arg1	glycosaminoglycans					455:472	xyloside-primed glycosaminoglycans	439:472	xyloside-primed glycosaminoglycans	439:472	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	26	theme	sulfate	791:797	arg1	proportion					718:727	the proportion	714:727	the proportion of heparan sulfate	714:746	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	3	26	theme	sulfate	791:797	arg1	complexity					756:765	the complexity	752:765	the complexity of chondroitin/dermatan sulfate	752:797	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	5	27	from	concentrations	1593:1606	arg1	xylosides					1547:1555	xylosides	1547:1555	xylosides of different types and at different concentrations	1547:1606	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	4	28	theme	heparan	967:973	arg1	sulfate					975:981	the heparan sulfate	963:981	the heparan sulfate	963:981	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	29	theme	heparan	1326:1332	arg1	sulfate					1334:1340	the heparan sulfate	1322:1340	the heparan sulfate	1322:1340	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	30	theme	xyloside	1191:1198	arg1	concentrations					1173:1186	increasing concentrations	1162:1186	increasing concentrations of xyloside	1162:1198	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	31	theme	6S-sulfated	1097:1107	arg1	disaccharides					1128:1140	6S-sulfated and/or nonsulfated disaccharides	1097:1140	6S-sulfated and/or nonsulfated disaccharides	1097:1140	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	3	32	theme	chondroitin/dermatan	770:789	arg1	sulfate					791:797	chondroitin/dermatan sulfate	770:797	chondroitin/dermatan sulfate	770:797	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	5	33	from	amounts	1453:1459	arg1	cells					1493:1497	living cells	1486:1497	living cells	1486:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	0	34	theme	glycosaminoglycans	29:46	arg1	structure					16:24	the structure	12:24	the structure of glycosaminoglycans	12:46	Fine-tuning the structure of glycosaminoglycans in living cells using xylosides.
29800297	1	35	from	impact	218:223	arg1	structure					259:267	the detailed glycosaminoglycan structure	228:267	the detailed glycosaminoglycan structure	228:267	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	2	36	dep	sulfate	498:504	arg1	proportion					539:548	proportion	539:548	proportion	539:548	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	2	36	dep	sulfate	498:504	arg1	composition					567:577	disaccharide composition	554:577	disaccharide composition	554:577	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	2	36	dep	sulfate	498:504	arg1	the					486:488	the	486:488	the	486:488	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	37	theme	xyloside	661:668	arg1	concentration					670:682	the xyloside concentration	657:682	the xyloside concentration	657:682	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	4	38	theme	xyloside	887:894	arg1	concentration					896:908	the xyloside concentration	883:908	the xyloside concentration	883:908	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	39	theme	disaccharides	1283:1295	arg1	proportion					1257:1266	the proportion	1253:1266	the proportion of nonsulfated disaccharides	1253:1295	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	0	40	theme	living	51:56	arg1	cells					58:62	living cells	51:62	living cells using xylosides	51:78	Fine-tuning the structure of glycosaminoglycans in living cells using xylosides.
29800297	2	41	theme	cell	400:403	arg1	type					405:408	the cell type	396:408	the cell type	396:408	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	42	theme	xyloside	696:703	arg1	concentration					670:682	the xyloside concentration	657:682	the xyloside concentration	657:682	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	3	42	theme	xyloside	696:703	arg1	type					688:691	type	688:691	type	688:691	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	4	43	theme	increasing	1162:1171	arg1	concentrations					1173:1186	increasing concentrations	1162:1186	increasing concentrations of xyloside	1162:1198	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	44	theme	xyloside	1376:1383	arg1	concentrations					1358:1371	increasing concentrations	1347:1371	increasing concentrations of xyloside	1347:1383	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	45	theme	chondroitin/dermatan	836:855	arg1	sulfate					857:863	the chondroitin/dermatan sulfate	832:863	the chondroitin/dermatan sulfate	832:863	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	5	46	theme	living	1486:1491	arg1	cells					1493:1497	living cells	1486:1497	living cells	1486:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	5	47	theme	glycosaminoglycans	1464:1481	arg1	glycosaminoglycans					1464:1481	glycosaminoglycans	1464:1481	glycosaminoglycans in living cells	1464:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	5	47	theme	glycosaminoglycans	1464:1481	arg1	amounts					1453:1459	large amounts	1447:1459	large amounts of glycosaminoglycans in living cells	1447:1497	Our results indicate that it is feasible to not only produce large amounts of glycosaminoglycans in living cells but also to fine-tune their structures by using xylosides of different types and at different concentrations.
29800297	1	48	theme	xyloside-primed	133:147	arg1	glycosaminoglycans					149:166	xyloside-primed glycosaminoglycans	133:166	xyloside-primed glycosaminoglycans	133:166	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	1	49	theme	detailed	232:239	arg1	structure					259:267	the detailed glycosaminoglycan structure	228:267	the detailed glycosaminoglycan structure	228:267	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	2	50	theme	chondroitin/dermatan	510:529	arg1	sulfate					531:537	chondroitin/dermatan sulfate	510:537	chondroitin/dermatan sulfate	510:537	Here, we have systematically investigated how the xyloside concentration and the type of xyloside, as well as the cell type, influenced the structure of xyloside-primed glycosaminoglycans in terms of the heparan sulfate and chondroitin/dermatan sulfate proportion and disaccharide composition.
29800297	3	51	theme	cell	641:644	arg1	type					646:649	the cell type	637:649	the cell type	637:649	We found that although greatest influence was exerted by the cell type, both the xyloside concentration and type of xyloside impacted the proportion of heparan sulfate and the complexity of chondroitin/dermatan sulfate.
29800297	1	52	theme	glycosaminoglycans	149:166	arg1	secretion					120:128	secretion	120:128	secretion	120:128	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	1	52	theme	glycosaminoglycans	149:166	arg1	formation					106:114	formation	106:114	formation	106:114	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	1	53	theme	glycosaminoglycan	241:257	arg1	structure					259:267	the detailed glycosaminoglycan structure	228:267	the detailed glycosaminoglycan structure	228:267	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	4	54	theme	disaccharides	1014:1026	arg1	proportion					988:997	the proportion	984:997	the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate	984:1062	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	55	theme	chondroitin/dermatan	1035:1054	arg1	sulfate					1056:1062	the chondroitin/dermatan sulfate	1031:1062	the chondroitin/dermatan sulfate	1031:1062	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	56	theme	sulfate	857:863	arg1	composition					817:827	The disaccharide composition	800:827	The disaccharide composition of the chondroitin/dermatan sulfate	800:863	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	4	57	theme	nonsulfated	1271:1281	arg1	disaccharides					1283:1295	nonsulfated disaccharides	1271:1295	nonsulfated disaccharides	1271:1295	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
29800297	1	58	dep	formation	106:114	arg1	the					102:104	the	102:104	the	102:104	Xylosides can induce the formation and secretion of xyloside-primed glycosaminoglycans when administered to living cells; however, their impact on the detailed glycosaminoglycan structure remains unknown.
29800297	4	59	theme	disaccharides	1128:1140	arg1	proportions					1082:1092	the proportions	1078:1092	the proportions of 6S-sulfated and/or nonsulfated disaccharides	1078:1140	The disaccharide composition of the chondroitin/dermatan sulfate was influenced by the xyloside concentration and type of xyloside to a higher extent than that of the heparan sulfate; the proportion of 4S-sulfated disaccharides in the chondroitin/dermatan sulfate decreased and the proportions of 6S-sulfated and/or nonsulfated disaccharides increased both with increasing concentrations of xyloside and with increasing xyloside hydrophobicity, whereas the proportion of nonsulfated disaccharides was primarily altered in the heparan sulfate with increasing concentrations of xyloside.
31753409	4	0	theme	In	688:689	arg1	profiles					705:712	In vitro release profiles	688:712	In vitro release profiles of tannic acid	688:727	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	2	1	theme	tannic	386:391	arg1	TA					399:400	TA	399:400	TA	399:400	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	2	1	theme	tannic	386:391	arg1	acid					393:396	tannic acid	386:396	tannic acid (TA)	386:401	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	1	2	theme	growing	275:281	arg1	challenge					283:291	a growing challenge	273:291	a growing challenge in biomedical field	273:311	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	4	3	theme	ionic	844:848	arg1	cross-links					850:860	Mg2+ ionic cross-links	839:860	Mg2+ ionic cross-links	839:860	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	3	4	theme	scanning	521:528	arg1	SEM					551:553	SEM	551:553	SEM	551:553	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	4	theme	scanning	521:528	arg1	microscopy					539:548	scanning electron microscopy	521:548	scanning electron microscopy (SEM)	521:554	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	5	theme	chemical	561:568	arg1	structure					570:578	chemical structure	561:578	chemical structure	561:578	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	1	6	theme	recurrence	186:195	arg1	rate					178:181	a high rate	171:181	a high rate of recurrence	171:195	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	0	7	theme	biomedical	109:118	arg1	applications					120:131	biomedical applications	109:131	biomedical applications	109:131	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	5	8	theme	strong	947:952	arg1	activity					968:975	strong antibacterial activity	947:975	strong antibacterial activity	947:975	The BC-TA-Mg composites also displayed strong antibacterial activity against S. aureus, E. coli and P. aeruginosa.
31753409	7	9	theme	BC-TA-Mg	1195:1202	arg1	composites					1204:1213	the BC-TA-Mg composites	1191:1213	the BC-TA-Mg composites	1191:1213	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	7	9	theme	BC-TA-Mg	1195:1202	arg1	material					1236:1243	a very promising material	1219:1243	a very promising material for combating biofilm-associated infections in biomedical and public health fields	1219:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	4	10	theme	release	697:703	arg1	profiles					705:712	In vitro release profiles	688:712	In vitro release profiles of tannic acid	688:727	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	1	11	theme	biomedical	296:305	arg1	field					307:311	biomedical field	296:311	biomedical field	296:311	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	7	12	theme	promising	1226:1234	arg1	composites					1204:1213	the BC-TA-Mg composites	1191:1213	the BC-TA-Mg composites	1191:1213	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	7	12	theme	promising	1226:1234	arg1	material					1236:1243	a very promising material	1219:1243	a very promising material for combating biofilm-associated infections in biomedical and public health fields	1219:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	4	13	dep	In	688:689	arg1	vitro					691:695	vitro	691:695	vitro	691:695	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	4	14	theme	composite	816:824	arg1	BC-TA					826:830	composite BC-TA	816:830	composite BC-TA	816:830	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	6	15	theme	biofilm	1070:1076	arg1	formation					1078:1086	biofilm formation	1070:1086	biofilm formation of S. aureus and P. aeruginosa	1070:1117	Moreover, the composites significantly reduced biofilm formation of S. aureus and P. aeruginosa after 24 h incubation by ∼80% and ∼87%, respectively.
31753409	7	16	theme	public	1307:1312	arg1	health					1314:1319	public health	1307:1319	public health	1307:1319	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	0	17	theme	biocompatible	2:14	arg1	composite					48:56	A biocompatible bacterial cellulose/tannic acid composite	0:56	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications	0:131	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	4	18	theme	Mg2+	839:842	arg1	cross-links					850:860	Mg2+ ionic cross-links	839:860	Mg2+ ionic cross-links	839:860	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	2	19	theme	biocompatible	331:343	arg1	composite					345:353	a biocompatible composite	329:353	a biocompatible composite	329:353	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	3	20	dep	Fourier	602:608	arg1	transform					610:618	transform	610:618	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS)	610:685	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	5	21	theme	BC-TA-Mg	912:919	arg1	composites					921:930	The BC-TA-Mg composites	908:930	The BC-TA-Mg composites	908:930	The BC-TA-Mg composites also displayed strong antibacterial activity against S. aureus, E. coli and P. aeruginosa.
31753409	1	22	theme	formidable	215:224	arg1	resistance					226:235	formidable resistance	215:235	formidable resistance to current antibiotics	215:258	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	0	23	theme	cellulose/tannic	26:41	arg1	composite					48:56	A biocompatible bacterial cellulose/tannic acid composite	0:56	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications	0:131	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	4	24	theme	Mg2+	747:750	arg1	cross-links					752:762	the Mg2+ cross-links	743:762	the Mg2+ cross-links	743:762	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	7	25	theme	biofilm-associated	1259:1276	arg1	infections					1278:1287	biofilm-associated infections	1259:1287	biofilm-associated infections in biomedical and public health fields	1259:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	0	26	theme	bacterial	16:24	arg1	composite					48:56	A biocompatible bacterial cellulose/tannic acid composite	0:56	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications	0:131	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	5	27	theme	antibacterial	954:966	arg1	activity					968:975	strong antibacterial activity	947:975	strong antibacterial activity	947:975	The BC-TA-Mg composites also displayed strong antibacterial activity against S. aureus, E. coli and P. aeruginosa.
31753409	3	28	theme	X-ray	653:657	arg1	XPS					682:684	XPS	682:684	XPS	682:684	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	28	theme	X-ray	653:657	arg1	spectra					673:679	X-ray photoelectron spectra	653:679	X-ray photoelectron spectra (XPS)	653:685	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	1	29	theme	Biofilm-associated	134:151	arg1	infections					153:162	Biofilm-associated infections	134:162	Biofilm-associated infections	134:162	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	3	30	theme	electron	530:537	arg1	SEM					551:553	SEM	551:553	SEM	551:553	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	30	theme	electron	530:537	arg1	microscopy					539:548	scanning electron microscopy	521:548	scanning electron microscopy (SEM)	521:554	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	31	theme	photoelectron	659:671	arg1	XPS					682:684	XPS	682:684	XPS	682:684	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	3	31	theme	photoelectron	659:671	arg1	spectra					673:679	X-ray photoelectron spectra	653:679	X-ray photoelectron spectra (XPS)	653:685	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	1	32	theme	current	240:246	arg1	antibiotics					248:258	current antibiotics	240:258	current antibiotics	240:258	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	3	33	dep	transform	610:618	arg1	infrared					620:627	infrared	620:627	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS)	610:685	The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS).
31753409	2	34	theme	bacterial	416:424	arg1	BC					437:438	BC	437:438	BC	437:438	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	2	34	theme	bacterial	416:424	arg1	cellulose					426:434	bacterial cellulose	416:434	bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes	416:483	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	0	35	theme	acid	43:46	arg1	composite					48:56	A biocompatible bacterial cellulose/tannic acid composite	0:56	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications	0:131	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	4	36	attach	released	880:887	arg1	hydrogel					898:905	the hydrogel	894:905	the hydrogel	894:905	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	4	36	attach	released	880:887	arg2	TA					873:874	TA	873:874	TA	873:874	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	1	37	from	challenge	283:291	arg1	field					307:311	biomedical field	296:311	biomedical field	296:311	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	4	38	theme	BC	799:800	arg1	matrix					802:807	BC matrix	799:807	BC matrix	799:807	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	0	39	theme	antibacterial	63:75	arg1	activities					94:103	antibacterial and anti-biofilm activities	63:103	activities	94:103	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	6	40	theme	aeruginosa	1108:1117	arg1	formation					1078:1086	biofilm formation	1070:1086	biofilm formation of S. aureus and P. aeruginosa	1070:1117	Moreover, the composites significantly reduced biofilm formation of S. aureus and P. aeruginosa after 24 h incubation by ∼80% and ∼87%, respectively.
31753409	7	41	theme	biomedical	1292:1301	arg1	fields					1321:1326	biomedical and public health fields	1292:1326	biomedical and public health fields	1292:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	4	42	from	matrix	802:807	arg1	release					780:786	the release	776:786	the release of TA from BC matrix	776:807	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	7	43	from	infections	1278:1287	arg1	fields					1321:1326	biomedical and public health fields	1292:1326	biomedical and public health fields	1292:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	4	44	theme	acid	724:727	arg1	profiles					705:712	In vitro release profiles	688:712	In vitro release profiles of tannic acid	688:727	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	6	45	theme	24 h	1125:1128	arg1	incubation					1130:1139	24 h incubation	1125:1139	24 h incubation	1125:1139	Moreover, the composites significantly reduced biofilm formation of S. aureus and P. aeruginosa after 24 h incubation by ∼80% and ∼87%, respectively.
31753409	6	46	theme	aureus	1094:1099	arg1	formation					1078:1086	biofilm formation	1070:1086	biofilm formation of S. aureus and P. aeruginosa	1070:1117	Moreover, the composites significantly reduced biofilm formation of S. aureus and P. aeruginosa after 24 h incubation by ∼80% and ∼87%, respectively.
31753409	4	47	theme	tannic	717:722	arg1	acid					724:727	tannic acid	717:727	tannic acid	717:727	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	0	48	theme	anti-biofilm	81:92	arg1	activities					94:103	antibacterial and anti-biofilm activities	63:103	activities	94:103	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	7	49	theme	health	1314:1319	arg1	fields					1321:1326	biomedical and public health fields	1292:1326	biomedical and public health fields	1292:1326	As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
31753409	1	50	theme	high	173:176	arg1	rate					178:181	a high rate	171:181	a high rate of recurrence	171:195	Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field.
31753409	2	51	theme	anti-biofilm	463:474	arg1	purposes					476:483	antimicrobial and anti-biofilm purposes	445:483	purposes	476:483	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31753409	4	52	theme	TA	791:792	arg1	release					780:786	the release	776:786	the release of TA from BC matrix	776:807	In vitro release profiles of tannic acid revealed that the Mg2+ cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg2+ ionic cross-links, thus more TA was released from the hydrogel.
31753409	0	53	with	composite	48:56	arg1	activities					94:103	antibacterial and anti-biofilm activities	63:103	activities	94:103	A biocompatible bacterial cellulose/tannic acid composite with antibacterial and anti-biofilm activities for biomedical applications.
31753409	2	54	theme	antimicrobial	445:457	arg1	purposes					476:483	antimicrobial and anti-biofilm purposes	445:483	purposes	476:483	In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl2 to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes.
31421611	7	0	theme	starch	1131:1136	arg1	yield					1122:1126	The extraction yield	1107:1126	The extraction yield of starch	1107:1136	The extraction yield of starch was not significantly affected by ultrasound conditions.
31421611	1	1	theme	due	162:164	arg1	industry					153:160	the food industry	144:160	the food industry due to its unique functional properties	144:200	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	6	2	theme	statistical	960:970	arg1	tests					972:976	statistical tests	960:976	statistical tests	960:976	Based on statistical tests, the Logistic model was found to fit well to the extraction kinetics of amylose, and the Peleg model fit well to that of starch.
31421611	10	3	theme	high-intensity	1572:1585	arg1	low-frequency					1587:1599	high-intensity low-frequency	1572:1599	high-intensity low-frequency	1572:1599	This study demonstrates that high-amylose starch can be obtained using ultrasound extraction from Radix Puerariae at high-intensity low-frequency.
31421611	6	4	theme	extraction	1027:1036	arg1	kinetics					1038:1045	the extraction kinetics	1023:1045	the extraction kinetics of amylose	1023:1056	Based on statistical tests, the Logistic model was found to fit well to the extraction kinetics of amylose, and the Peleg model fit well to that of starch.
31421611	9	5	theme	increased	1321:1329	arg1	content					1339:1345	the increased amylose content	1317:1345	the increased amylose content	1317:1345	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	3	6	theme	intensity	369:377	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	6	theme	intensity	369:377	arg1	behaviour					539:547	in vitro digestion behaviour	520:547	in vitro digestion behaviour	520:547	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	6	theme	intensity	369:377	arg1	crystallinity					505:517	crystallinity	505:517	crystallinity	505:517	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	6	theme	intensity	369:377	arg1	properties					561:570	gelling properties	553:570	gelling properties of starches	553:582	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	6	theme	intensity	369:377	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	9	7	theme	starch	1425:1430	arg1	content					1378:1384	the content	1374:1384	the content of slowly digestible starch, resistant starch, and gelling hardness	1374:1452	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	4	8	theme	extraction	725:734	arg1	rate					736:739	the extraction rate	721:739	the extraction rate	721:739	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	5	9	theme	extraction	914:923	arg1	rate					925:928	the extraction rate	910:928	the extraction rate of starch	910:938	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	9	10	theme	amylose	1331:1337	arg1	content					1339:1345	the increased amylose content	1317:1345	the increased amylose content	1317:1345	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	2	11	theme	ultrasound	320:329	arg1	extraction					331:340	ultrasound extraction	320:340	ultrasound extraction	320:340	In this study, high-amylose starch was successfully extracted from Radix Puerariae using ultrasound extraction.
31421611	4	12	dep	rate	667:670	arg1	the					652:654	the	652:654	the	652:654	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	3	13	theme	in	520:521	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	13	theme	in	520:521	arg1	behaviour					539:547	in vitro digestion behaviour	520:547	in vitro digestion behaviour	520:547	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	4	14	theme	amylose	687:693	arg1	rate					667:670	extraction rate	656:670	extraction rate	656:670	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	4	14	theme	amylose	687:693	arg1	content					676:682	content	676:682	content	676:682	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	1	15	theme	great	128:132	arg1	demand					134:139	great demand	128:139	great demand	128:139	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	4	16	theme	24.46 kW/m2	804:814	arg1	intensity					791:799	an intensity	788:799	an intensity of 24.46 kW/m2	788:814	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	3	17	from	compositions	491:502	arg1	kinetics					468:475	extraction kinetics	457:475	extraction kinetics	457:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	5	18	theme	starch	933:938	arg1	rate					925:928	the extraction rate	910:928	the extraction rate of starch	910:938	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	4	19	theme	increasing	630:639	arg1	intensity					641:649	the increasing intensity	626:649	the increasing intensity	626:649	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	3	20	theme	ultrasound	358:367	arg1	intensity					369:377	ultrasound intensity	358:377	ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2)	358:422	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	9	21	theme	starch	1407:1412	arg1	content					1378:1384	the content	1374:1384	the content of slowly digestible starch, resistant starch, and gelling hardness	1374:1452	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	1	22	theme	unique	173:178	arg1	properties					191:200	its unique functional properties	169:200	its unique functional properties	169:200	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	1	23	theme	High-amylose	102:113	arg1	starch					115:120	High-amylose starch	102:120	High-amylose starch	102:120	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	0	24	from	Puerariae	45:53	arg1	Extraction					0:9	Extraction	0:9	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.	0:100	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.
31421611	1	25	theme	functional	180:189	arg1	properties					191:200	its unique functional properties	169:200	its unique functional properties	169:200	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	5	26	dep	rate	867:870	arg1	the					852:854	the	852:854	the	852:854	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	5	27	theme	increasing	826:835	arg1	low-frequency					837:849	the increasing low-frequency	822:849	the increasing low-frequency	822:849	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	8	28	with	high-purity	1221:1231	arg1	content					1240:1246	a content	1238:1246	a content of more than 99% dry basis and an unchanged crystallinity	1238:1304	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	0	29	theme	starch	27:32	arg1	Extraction					0:9	Extraction	0:9	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.	0:100	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.
31421611	1	30	from	demand	134:139	arg1	industry					153:160	the food industry	144:160	the food industry due to its unique functional properties	144:200	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	8	31	theme	unchanged	1282:1290	arg1	crystallinity					1292:1304	an unchanged crystallinity	1279:1304	an unchanged crystallinity	1279:1304	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	0	32	theme	high-amylose	14:25	arg1	starch					27:32	high-amylose starch	14:32	high-amylose starch	14:32	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.
31421611	8	33	theme	dry	1265:1267	arg1	basis					1269:1273	more than 99% dry basis	1251:1273	more than 99% dry basis	1251:1273	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	3	34	theme	digestion	529:537	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	34	theme	digestion	529:537	arg1	behaviour					539:547	in vitro digestion behaviour	520:547	in vitro digestion behaviour	520:547	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	35	theme	chemical	482:489	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	7	36	theme	ultrasound	1172:1181	arg1	conditions					1183:1192	ultrasound conditions	1172:1192	ultrasound conditions	1172:1192	The extraction yield of starch was not significantly affected by ultrasound conditions.
31421611	8	37	dep	basis	1269:1273	arg1	%					1263:1263	%	1263:1263	%	1263:1263	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	9	38	theme	content	1378:1384	arg1	increase					1362:1369	an increase	1359:1369	an increase of the content of slowly digestible starch, resistant starch, and gelling hardness	1359:1452	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	4	39	theme	extraction	656:665	arg1	rate					667:670	extraction rate	656:670	extraction rate	656:670	It was shown that with the increasing intensity, the extraction rate and content of amylose increased, but for starch the extraction rate increased initially until reached a plateau at an intensity of 24.46 kW/m2.
31421611	8	40	dep	%	1263:1263	arg1	99					1261:1262	99	1261:1262	99	1261:1262	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	3	41	theme	starches	575:582	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	41	theme	starches	575:582	arg1	behaviour					539:547	in vitro digestion behaviour	520:547	in vitro digestion behaviour	520:547	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	41	theme	starches	575:582	arg1	crystallinity					505:517	crystallinity	505:517	crystallinity	505:517	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	41	theme	starches	575:582	arg1	properties					561:570	gelling properties	553:570	gelling properties of starches	553:582	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	10	42	from	Puerariae	1559:1567	arg1	extraction					1537:1546	ultrasound extraction	1526:1546	ultrasound extraction from Radix Puerariae	1526:1567	This study demonstrates that high-amylose starch can be obtained using ultrasound extraction from Radix Puerariae at high-intensity low-frequency.
31421611	1	43	theme	limited	215:221	arg1	source					223:228	very limited source	210:228	very limited source	210:228	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	10	44	theme	ultrasound	1526:1535	arg1	extraction					1537:1546	ultrasound extraction	1526:1546	ultrasound extraction from Radix Puerariae	1526:1567	This study demonstrates that high-amylose starch can be obtained using ultrasound extraction from Radix Puerariae at high-intensity low-frequency.
31421611	10	45	theme	high-amylose	1484:1495	arg1	starch					1497:1502	high-amylose starch	1484:1502	high-amylose starch	1484:1502	This study demonstrates that high-amylose starch can be obtained using ultrasound extraction from Radix Puerariae at high-intensity low-frequency.
31421611	3	46	theme	gelling	553:559	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	46	theme	gelling	553:559	arg1	properties					561:570	gelling properties	553:570	gelling properties of starches	553:582	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	6	47	theme	amylose	1050:1056	arg1	kinetics					1038:1045	the extraction kinetics	1023:1045	the extraction kinetics of amylose	1023:1056	Based on statistical tests, the Logistic model was found to fit well to the extraction kinetics of amylose, and the Peleg model fit well to that of starch.
31421611	3	48	theme	extraction	457:466	arg1	kinetics					468:475	extraction kinetics	457:475	extraction kinetics	457:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	7	49	theme	extraction	1111:1120	arg1	yield					1122:1126	The extraction yield	1107:1126	The extraction yield of starch	1107:1136	The extraction yield of starch was not significantly affected by ultrasound conditions.
31421611	9	50	theme	gelling	1437:1443	arg1	hardness					1445:1452	gelling hardness	1437:1452	gelling hardness	1437:1452	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	9	51	theme	digestible	1396:1405	arg1	starch					1407:1412	slowly digestible starch	1389:1412	slowly digestible starch	1389:1412	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	3	52	dep	intensity	369:377	arg1	20					439:440	20	439:440	20	439:440	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	52	dep	intensity	369:377	arg1	25.38 kW/m2					411:421	25.38 kW/m2	411:421	25.38 kW/m2	411:421	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	52	dep	intensity	369:377	arg1	24.46					401:405	24.46	401:405	24.46	401:405	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	52	dep	intensity	369:377	arg1	45 kHz					446:451	45 kHz	446:451	45 kHz	446:451	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	8	53	theme	basis	1269:1273	arg1	content					1240:1246	a content	1238:1246	a content of more than 99% dry basis and an unchanged crystallinity	1238:1304	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	8	54	theme	crystallinity	1292:1304	arg1	content					1240:1246	a content	1238:1246	a content of more than 99% dry basis and an unchanged crystallinity	1238:1304	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	5	55	theme	amylose	887:893	arg1	rate					867:870	extraction rate	856:870	extraction rate	856:870	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	5	55	theme	amylose	887:893	arg1	content					876:882	content	876:882	content	876:882	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
31421611	6	56	theme	Logistic	983:990	arg1	model					992:996	the Logistic model	979:996	the Logistic model	979:996	Based on statistical tests, the Logistic model was found to fit well to the extraction kinetics of amylose, and the Peleg model fit well to that of starch.
31421611	1	57	contain	has	206:208	arg1	starch					115:120	High-amylose starch	102:120	High-amylose starch	102:120	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	1	57	contain	has	206:208	arg2	source					223:228	very limited source	210:228	very limited source	210:228	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	2	58	theme	high-amylose	246:257	arg1	starch					259:264	high-amylose starch	246:264	high-amylose starch	246:264	In this study, high-amylose starch was successfully extracted from Radix Puerariae using ultrasound extraction.
31421611	3	59	dep	in	520:521	arg1	vitro					523:527	vitro	523:527	vitro	523:527	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	6	60	theme	Peleg	1067:1071	arg1	model					1073:1077	the Peleg model	1063:1077	the Peleg model	1063:1077	Based on statistical tests, the Logistic model was found to fit well to the extraction kinetics of amylose, and the Peleg model fit well to that of starch.
31421611	8	61	contain	has	1215:1217	arg2	high-purity					1221:1231	a high-purity	1219:1231	a high-purity with a content of more than 99% dry basis and an unchanged crystallinity	1219:1304	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	8	61	contain	has	1215:1217	arg1	starch					1208:1213	The obtained starch	1195:1213	The obtained starch	1195:1213	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	0	62	theme	low-frequency	76:88	arg1	ultrasound					90:99	high-intensity low-frequency ultrasound	61:99	high-intensity low-frequency ultrasound	61:99	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.
31421611	3	63	from	effects	347:353	arg1	kinetics					468:475	extraction kinetics	457:475	extraction kinetics	457:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	8	64	theme	obtained	1199:1206	arg1	starch					1208:1213	The obtained starch	1195:1213	The obtained starch	1195:1213	The obtained starch has a high-purity with a content of more than 99% dry basis and an unchanged crystallinity.
31421611	9	65	theme	resistant	1415:1423	arg1	starch					1425:1430	resistant starch	1415:1430	resistant starch	1415:1430	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	1	66	theme	food	148:151	arg1	industry					153:160	the food industry	144:160	the food industry due to its unique functional properties	144:200	High-amylose starch is in great demand in the food industry due to its unique functional properties but has very limited source.
31421611	3	67	theme	frequency	428:436	arg1	effects					347:353	The effects	343:353	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics	343:475	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	67	theme	frequency	428:436	arg1	behaviour					539:547	in vitro digestion behaviour	520:547	in vitro digestion behaviour	520:547	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	67	theme	frequency	428:436	arg1	crystallinity					505:517	crystallinity	505:517	crystallinity	505:517	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	67	theme	frequency	428:436	arg1	properties					561:570	gelling properties	553:570	gelling properties of starches	553:582	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	3	67	theme	frequency	428:436	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effects of ultrasound intensity (15.29, 20.38, 22.93, 24.46 and 25.38 kW/m2) and frequency (20 and 45 kHz) on extraction kinetics, and chemical compositions, crystallinity, in vitro digestion behaviour and gelling properties of starches were investigated.
31421611	9	68	theme	hardness	1445:1452	arg1	content					1378:1384	the content	1374:1384	the content of slowly digestible starch, resistant starch, and gelling hardness	1374:1452	Moreover, the increased amylose content resulted in an increase of the content of slowly digestible starch, resistant starch, and gelling hardness.
31421611	0	69	theme	high-intensity	61:74	arg1	ultrasound					90:99	high-intensity low-frequency ultrasound	61:99	high-intensity low-frequency ultrasound	61:99	Extraction of high-amylose starch from Radix Puerariae using high-intensity low-frequency ultrasound.
31421611	5	70	theme	extraction	856:865	arg1	rate					867:870	extraction rate	856:870	extraction rate	856:870	With the increasing low-frequency, the extraction rate and content of amylose increased, but the extraction rate of starch decreased.
30503788	10	0	theme	metabolic	1438:1446	arg1	microbiota					1417:1426	the gut microbiota	1409:1426	the gut microbiota	1409:1426	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	0	theme	metabolic	1438:1446	arg1	processes					1448:1456	balanced metabolic processes	1429:1456	balanced metabolic processes	1429:1456	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	7	1	theme	Bacteroides	975:985	arg1	abundance					962:970	the relative abundance	949:970	the relative abundance of Bacteroides	949:985	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	8	2	from	phylotype	996:1004	arg1	microbiota					1040:1049	the gut microbiota	1032:1049	the gut microbiota that responded to WGP	1032:1071	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	4	3	from	effects	421:427	arg1	diversity					469:477	the diversity	465:477	the diversity of the gut microbiota in mice with antibiotic-associated diarrhea	465:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	4	4	theme	gut	486:488	arg1	microbiota					490:499	the gut microbiota	482:499	the gut microbiota	482:499	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	5	5	theme	gut	636:638	arg1	microbiota					640:649	the gut microbiota	632:649	the gut microbiota	632:649	Compared to diarrhea mice, WGP significantly changed the composition and diversity of the gut microbiota.
30503788	7	6	theme	relative	953:960	arg1	abundance					962:970	the relative abundance	949:970	the relative abundance of Bacteroides	949:985	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	4	7	theme	polysaccharides	440:454	arg1	effects					421:427	the effects	417:427	the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea	417:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	10	8	theme	balanced	1429:1436	arg1	microbiota					1417:1426	the gut microbiota	1409:1426	the gut microbiota	1409:1426	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	8	theme	balanced	1429:1436	arg1	processes					1448:1456	balanced metabolic processes	1429:1456	balanced metabolic processes	1429:1456	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	0	9	from	Effects	0:6	arg1	microbiota					52:61	the gut microbiota	44:61	the gut microbiota in mice with antibiotic-associated diarrhea	44:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	5	10	theme	microbiota	640:649	arg1	diversity					619:627	diversity	619:627	diversity	619:627	Compared to diarrhea mice, WGP significantly changed the composition and diversity of the gut microbiota.
30503788	5	10	theme	microbiota	640:649	arg1	composition					603:613	composition	603:613	composition	603:613	Compared to diarrhea mice, WGP significantly changed the composition and diversity of the gut microbiota.
30503788	0	11	with	mice	66:69	arg1	diarrhea					98:105	antibiotic-associated diarrhea	76:105	antibiotic-associated diarrhea	76:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	9	12	theme	energy	1152:1157	arg1	metabolism					1159:1168	energy metabolism	1152:1168	energy metabolism	1152:1168	In addition, WGP also reversed carbohydrate, amino acid and energy metabolism to normal levels, thereby promoting the recovery of the mucosal structure.
30503788	7	13	theme	relative	866:873	arg1	abundance					875:883	the relative abundance	862:883	the relative abundance of Lactobacillus, Lactococcus, and Streptococcus	862:932	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	3	14	theme	antibiotic	354:363	arg1	use					365:367	antibiotic use	354:367	antibiotic use	354:367	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	4	15	from	diversity	469:477	arg1	mice					504:507	mice	504:507	mice with antibiotic-associated diarrhea	504:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	9	16	theme	normal	1173:1178	arg1	levels					1180:1185	normal levels	1173:1185	normal levels	1173:1185	In addition, WGP also reversed carbohydrate, amino acid and energy metabolism to normal levels, thereby promoting the recovery of the mucosal structure.
30503788	8	17	theme	key	992:994	arg1	Lactobacillus					1077:1089	Lactobacillus	1077:1089	Lactobacillus	1077:1089	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	8	17	theme	key	992:994	arg1	phylotype					996:1004	The key phylotype	988:1004	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP	988:1071	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	4	18	theme	study	392:396	arg1	objective					374:382	The objective	370:382	The objective of this study	370:396	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	2	19	theme	active	254:259	arg1	component					261:269	its primary active component	242:269	its primary active component	242:269	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	2	19	theme	active	254:259	arg1	polysaccharides					272:286	polysaccharides	272:286	polysaccharides	272:286	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	1	20	theme	most	163:166	arg1	countries					174:182	most Asian countries	163:182	most Asian countries	163:182	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	6	21	theme	phyla	772:776	arg1	abundance					755:763	the relative abundance	742:763	the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria	742:825	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	10	22	theme	gut	1413:1415	arg1	microbiota					1417:1426	the gut microbiota	1409:1426	the gut microbiota	1409:1426	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	22	theme	gut	1413:1415	arg1	processes					1448:1456	balanced metabolic processes	1429:1456	balanced metabolic processes	1429:1456	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	2	23	theme	primary	246:252	arg1	component					261:269	its primary active component	242:269	its primary active component	242:269	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	2	23	theme	primary	246:252	arg1	polysaccharides					272:286	polysaccharides	272:286	polysaccharides	272:286	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	6	24	theme	relative	684:691	arg1	abundance					693:701	the relative abundance	680:701	the relative abundance of the phylum Firmicutes	680:726	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	1	25	theme	Asian	168:172	arg1	countries					174:182	most Asian countries	163:182	most Asian countries	163:182	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	5	26	theme	diarrhea	558:565	arg1	mice					567:570	diarrhea mice	558:570	diarrhea mice	558:570	Compared to diarrhea mice, WGP significantly changed the composition and diversity of the gut microbiota.
30503788	5	27	dep	composition	603:613	arg1	the					599:601	the	599:601	the	599:601	Compared to diarrhea mice, WGP significantly changed the composition and diversity of the gut microbiota.
30503788	8	28	theme	beneficial	1009:1018	arg1	bacteria					1020:1027	beneficial bacteria	1009:1027	beneficial bacteria	1009:1027	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	4	29	with	mice	504:507	arg1	diarrhea					536:543	antibiotic-associated diarrhea	514:543	antibiotic-associated diarrhea	514:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	6	30	dep	Proteobacteria	793:806	arg1	Bacteroidetes					778:790	Bacteroidetes	778:790	Bacteroidetes	778:790	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	8	31	theme	bacteria	1020:1027	arg1	Lactobacillus					1077:1089	Lactobacillus	1077:1089	Lactobacillus	1077:1089	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	8	31	theme	bacteria	1020:1027	arg1	phylotype					996:1004	The key phylotype	988:1004	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP	988:1071	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	0	32	theme	ginseng	17:23	arg1	polysaccharides					25:39	Panax ginseng polysaccharides	11:39	Panax ginseng polysaccharides	11:39	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	4	33	theme	antibiotic-associated	514:534	arg1	diarrhea					536:543	antibiotic-associated diarrhea	514:543	antibiotic-associated diarrhea	514:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	7	34	theme	Streptococcus	920:932	arg1	abundance					875:883	the relative abundance	862:883	the relative abundance of Lactobacillus, Lactococcus, and Streptococcus	862:932	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	7	35	theme	genus	835:839	arg1	level					841:845	the genus level	831:845	the genus level	831:845	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	0	36	theme	Panax	11:15	arg1	polysaccharides					25:39	Panax ginseng polysaccharides	11:39	Panax ginseng polysaccharides	11:39	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	4	37	theme	ginseng	432:438	arg1	WGP					457:459	WGP	457:459	WGP	457:459	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	4	37	theme	ginseng	432:438	arg1	polysaccharides					440:454	ginseng polysaccharides	432:454	ginseng polysaccharides (WGP)	432:460	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	1	38	theme	Panax	108:112	arg1	plant					149:153	a traditional medicinal plant	125:153	a traditional medicinal plant used in most Asian countries to cure many diseases	125:204	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	1	38	theme	Panax	108:112	arg1	ginseng					114:120	Panax ginseng	108:120	Panax ginseng	108:120	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	1	39	theme	many	192:195	arg1	diseases					197:204	many diseases	192:204	many diseases	192:204	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	2	40	theme	ginseng	223:229	arg1	due					235:237	due	235:237	due	235:237	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	2	40	theme	ginseng	223:229	arg1	benefits					211:218	The benefits	207:218	The benefits of ginseng	207:229	The benefits of ginseng are due to its primary active component, polysaccharides.
30503788	10	41	from	diversity	1323:1331	arg1	mice					1358:1361	mice	1358:1361	mice with antibiotic-associated diarrhea	1358:1397	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	0	42	theme	polysaccharides	25:39	arg1	Effects					0:6	Effects	0:6	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea	0:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	0	43	theme	gut	48:50	arg1	microbiota					52:61	the gut microbiota	44:61	the gut microbiota in mice with antibiotic-associated diarrhea	44:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	4	44	theme	microbiota	490:499	arg1	diversity					469:477	the diversity	465:477	the diversity of the gut microbiota in mice with antibiotic-associated diarrhea	465:543	The objective of this study was to investigate the effects of ginseng polysaccharides (WGP) on the diversity of the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	3	45	theme	Gut	289:291	arg1	dysbiosis					304:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	3	45	theme	Gut	289:291	arg1	problem					329:335	a worldwide problem	317:335	a worldwide problem associating with antibiotic use	317:367	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	0	46	from	microbiota	52:61	arg1	mice					66:69	mice	66:69	mice with antibiotic-associated diarrhea	66:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
30503788	6	47	theme	relative	746:753	arg1	abundance					755:763	the relative abundance	742:763	the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria	742:825	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	10	48	with	mice	1358:1361	arg1	diarrhea					1390:1397	antibiotic-associated diarrhea	1368:1397	antibiotic-associated diarrhea	1368:1397	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	3	49	theme	microbiota	293:302	arg1	dysbiosis					304:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	3	49	theme	microbiota	293:302	arg1	problem					329:335	a worldwide problem	317:335	a worldwide problem associating with antibiotic use	317:367	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	9	50	theme	structure	1234:1242	arg1	recovery					1210:1217	the recovery	1206:1217	the recovery of the mucosal structure	1206:1242	In addition, WGP also reversed carbohydrate, amino acid and energy metabolism to normal levels, thereby promoting the recovery of the mucosal structure.
30503788	9	51	theme	mucosal	1226:1232	arg1	structure					1234:1242	the mucosal structure	1222:1242	the mucosal structure	1222:1242	In addition, WGP also reversed carbohydrate, amino acid and energy metabolism to normal levels, thereby promoting the recovery of the mucosal structure.
30503788	10	52	dep	composition	1307:1317	arg1	the					1303:1305	the	1303:1305	the	1303:1305	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	8	53	theme	gut	1036:1038	arg1	microbiota					1040:1049	the gut microbiota	1032:1049	the gut microbiota that responded to WGP	1032:1071	The key phylotype of beneficial bacteria in the gut microbiota that responded to WGP was Lactobacillus.
30503788	7	54	theme	Lactococcus	903:913	arg1	abundance					875:883	the relative abundance	862:883	the relative abundance of Lactobacillus, Lactococcus, and Streptococcus	862:932	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	10	55	theme	antibiotic-associated	1368:1388	arg1	diarrhea					1390:1397	antibiotic-associated diarrhea	1368:1397	antibiotic-associated diarrhea	1368:1397	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	56	theme	gut	1340:1342	arg1	microbiota					1344:1353	the gut microbiota	1336:1353	the gut microbiota	1336:1353	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	57	theme	mucosa	1492:1497	arg1	recovery					1476:1483	the recovery	1472:1483	the recovery of the mucosa	1472:1497	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	9	58	theme	amino	1137:1141	arg1	acid					1143:1146	amino acid	1137:1146	amino acid	1137:1146	In addition, WGP also reversed carbohydrate, amino acid and energy metabolism to normal levels, thereby promoting the recovery of the mucosal structure.
30503788	1	59	theme	traditional	127:137	arg1	plant					149:153	a traditional medicinal plant	125:153	a traditional medicinal plant used in most Asian countries to cure many diseases	125:204	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	1	59	theme	traditional	127:137	arg1	ginseng					114:120	Panax ginseng	108:120	Panax ginseng	108:120	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	7	60	theme	Lactobacillus	888:900	arg1	abundance					875:883	the relative abundance	862:883	the relative abundance of Lactobacillus, Lactococcus, and Streptococcus	862:932	At the genus level, WGP increased the relative abundance of Lactobacillus, Lactococcus, and Streptococcus, but decreased the relative abundance of Bacteroides.
30503788	10	61	theme	microbiota	1344:1353	arg1	diversity					1323:1331	diversity	1323:1331	diversity	1323:1331	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	10	61	theme	microbiota	1344:1353	arg1	composition					1307:1317	composition	1307:1317	composition	1307:1317	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	6	62	theme	Firmicutes	717:726	arg1	abundance					693:701	the relative abundance	680:701	the relative abundance of the phylum Firmicutes	680:726	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	1	63	theme	medicinal	139:147	arg1	plant					149:153	a traditional medicinal plant	125:153	a traditional medicinal plant used in most Asian countries to cure many diseases	125:204	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	1	63	theme	medicinal	139:147	arg1	ginseng					114:120	Panax ginseng	108:120	Panax ginseng	108:120	Panax ginseng is a traditional medicinal plant used in most Asian countries to cure many diseases.
30503788	10	64	from	composition	1307:1317	arg1	mice					1358:1361	mice	1358:1361	mice with antibiotic-associated diarrhea	1358:1397	Taken collectively, our results indicate that WGP altered the composition and diversity of the gut microbiota in mice with antibiotic-associated diarrhea, restored the gut microbiota, balanced metabolic processes, and promoted the recovery of the mucosa.
30503788	3	65	theme	worldwide	319:327	arg1	dysbiosis					304:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis	289:312	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	3	65	theme	worldwide	319:327	arg1	problem					329:335	a worldwide problem	317:335	a worldwide problem associating with antibiotic use	317:367	Gut microbiota dysbiosis is a worldwide problem associating with antibiotic use.
30503788	6	66	theme	phylum	710:715	arg1	Firmicutes					717:726	the phylum Firmicutes	706:726	the phylum Firmicutes	706:726	Specifically, WGP increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes, Proteobacteria and Actinobacteria.
30503788	0	67	theme	antibiotic-associated	76:96	arg1	diarrhea					98:105	antibiotic-associated diarrhea	76:105	antibiotic-associated diarrhea	76:105	Effects of Panax ginseng polysaccharides on the gut microbiota in mice with antibiotic-associated diarrhea.
29804430	0	0	theme	bacterial	72:80	arg1	cellulose					82:90	bacterial cellulose	72:90	bacterial cellulose	72:90	[Advances in the research of antibacterial composite dressings based on bacterial cellulose].
29804430	5	1	theme	research	696:703	arg1	trends					705:710	the research trends	692:710	the research trends of antibacterial composite dressings based on BC	692:759	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	0	2	from	[Advances	0:8	arg1	research					17:24	the research	13:24	the research of antibacterial composite dressings	13:61	[Advances in the research of antibacterial composite dressings based on bacterial cellulose].
29804430	3	3	theme	several	358:364	arg1	kinds					366:370	several kinds	358:370	several kinds of antibacterial agents	358:394	To gain antimicrobial activity, several kinds of antibacterial agents have been introduced into BC.
29804430	5	4	from	prospects	679:687	arg1	future					764:769	future	764:769	future	764:769	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	3	5	theme	antimicrobial	334:346	arg1	activity					348:355	antimicrobial activity	334:355	antimicrobial activity	334:355	To gain antimicrobial activity, several kinds of antibacterial agents have been introduced into BC.
29804430	4	6	theme	composite	460:468	arg1	dressings					470:478	the antibacterial composite dressings	442:478	the antibacterial composite dressings	442:478	However, all of the antibacterial composite dressings are still in the stage of experimental research.
29804430	5	7	theme	antibacterial	600:612	arg1	dressings					624:632	antibacterial composite dressings	600:632	antibacterial composite dressings based on BC	600:644	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	1	8	theme	wound	147:151	arg1	dressing					153:160	wound dressing	147:160	wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility	147:269	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	5	9	theme	antibacterial	715:727	arg1	dressings					739:747	antibacterial composite dressings	715:747	antibacterial composite dressings based on BC	715:759	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	5	10	theme	composite	614:622	arg1	dressings					624:632	antibacterial composite dressings	600:632	antibacterial composite dressings based on BC	600:644	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	3	11	theme	antibacterial	375:387	arg1	agents					389:394	antibacterial agents	375:394	antibacterial agents	375:394	To gain antimicrobial activity, several kinds of antibacterial agents have been introduced into BC.
29804430	5	12	theme	dressings	624:632	arg1	types					548:552	the types	544:552	the types	544:552	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	5	12	theme	dressings	624:632	arg1	mechanism					569:577	antibacterial mechanism	555:577	antibacterial mechanism	555:577	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	5	12	theme	dressings	624:632	arg1	shortcomings					584:595	shortcomings	584:595	shortcomings	584:595	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	2	13	theme	antimicrobial	302:314	arg1	activity					316:323	no antimicrobial activity	299:323	no antimicrobial activity	299:323	Whereas BC itself exhibits no antimicrobial activity.
29804430	1	14	theme	due	162:164	arg1	dressing					153:160	wound dressing	147:160	wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility	147:269	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	3	15	theme	agents	389:394	arg1	kinds					366:370	several kinds	358:370	several kinds of antibacterial agents	358:394	To gain antimicrobial activity, several kinds of antibacterial agents have been introduced into BC.
29804430	5	16	theme	antibacterial	555:567	arg1	mechanism					569:577	antibacterial mechanism	555:577	antibacterial mechanism	555:577	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	1	17	theme	Bacterial	94:102	arg1	material					134:141	a promising material	122:141	a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility	122:269	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	1	17	theme	Bacterial	94:102	arg1	BC					115:116	BC	115:116	BC	115:116	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	1	17	theme	Bacterial	94:102	arg1	cellulose					104:112	Bacterial cellulose	94:112	Bacterial cellulose (BC)	94:117	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	1	18	theme	predominant	173:183	arg1	capacity					199:206	its predominant water binding capacity	169:206	its predominant water binding capacity	169:206	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	0	19	theme	antibacterial	29:41	arg1	dressings					53:61	antibacterial composite dressings	29:61	antibacterial composite dressings	29:61	[Advances in the research of antibacterial composite dressings based on bacterial cellulose].
29804430	1	20	theme	water	185:189	arg1	capacity					199:206	its predominant water binding capacity	169:206	its predominant water binding capacity	169:206	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	4	21	theme	research	519:526	arg1	stage					497:501	the stage	493:501	the stage of experimental research	493:526	However, all of the antibacterial composite dressings are still in the stage of experimental research.
29804430	4	22	theme	antibacterial	446:458	arg1	dressings					470:478	the antibacterial composite dressings	442:478	the antibacterial composite dressings	442:478	However, all of the antibacterial composite dressings are still in the stage of experimental research.
29804430	1	23	theme	binding	191:197	arg1	capacity					199:206	its predominant water binding capacity	169:206	its predominant water binding capacity	169:206	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	0	24	theme	dressings	53:61	arg1	research					17:24	the research	13:24	the research of antibacterial composite dressings	13:61	[Advances in the research of antibacterial composite dressings based on bacterial cellulose].
29804430	4	25	theme	experimental	506:517	arg1	research					519:526	experimental research	506:526	experimental research	506:526	However, all of the antibacterial composite dressings are still in the stage of experimental research.
29804430	0	26	theme	composite	43:51	arg1	dressings					53:61	antibacterial composite dressings	29:61	antibacterial composite dressings	29:61	[Advances in the research of antibacterial composite dressings based on bacterial cellulose].
29804430	5	27	theme	composite	729:737	arg1	dressings					739:747	antibacterial composite dressings	715:747	antibacterial composite dressings based on BC	715:759	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	1	28	theme	mechanical	209:218	arg1	property					220:227	mechanical property	209:227	mechanical property	209:227	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	5	29	theme	dressings	739:747	arg1	trends					705:710	the research trends	692:710	the research trends of antibacterial composite dressings based on BC	692:759	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29804430	1	30	theme	promising	124:132	arg1	material					134:141	a promising material	122:141	a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility	122:269	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	1	30	theme	promising	124:132	arg1	cellulose					104:112	Bacterial cellulose	94:112	Bacterial cellulose (BC)	94:117	Bacterial cellulose (BC) is a promising material for wound dressing due to its predominant water binding capacity, mechanical property, biodegradability, and histocompatibility.
29804430	5	31	theme	trends	705:710	arg1	prospects					679:687	prospects	679:687	prospects of the research trends of antibacterial composite dressings based on BC in future	679:769	In this paper, the types, antibacterial mechanism, and shortcomings of antibacterial composite dressings based on BC are summarized, in order to make prospects of the research trends of antibacterial composite dressings based on BC in future.
29567498	7	0	theme	release	1202:1208	arg1	mechanism					1210:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
29567498	1	1	theme	study	346:350	arg1	purpose					330:336	the main purpose	321:336	the main purpose of this study	321:350	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	1	1	theme	study	346:350	arg1	Development					137:147	Development	137:147	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design	137:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	3	2	theme	polymer	508:514	arg1	ratio					516:520	polymer ratio	508:520	polymer ratio	508:520	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	5	3	from	concentration	968:980	arg1	medium					1012:1017	cross-linking medium	998:1017	cross-linking medium	998:1017	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	4	theme	diclofenac	269:278	arg1	sodium					280:285	diclofenac sodium	269:285	diclofenac sodium using 32 full factorial design	269:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	0	5	theme	sodium	98:103	arg1	release					76:82	extended release	67:82	extended release of diclofenac sodium	67:103	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	6	6	with	period	1111:1116	arg1	f2=80.13					1130:1137	f2=80.13	1130:1137	f2=80.13	1130:1137	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	6	7	theme	drug	1083:1086	arg1	profile					1096:1102	an extended drug release profile	1071:1102	an extended drug release profile	1071:1102	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	1	8	theme	TSG	171:173	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	5	9	from	increase	952:959	arg1	ratio					923:927	the ratio	919:927	the ratio of h-Pmaa-g-GG:TSG	919:946	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	5	9	from	increase	952:959	arg1	concentration					968:980	the concentration	964:980	the concentration of Ca2+ ions in cross-linking medium	964:1017	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	10	theme	sodium	280:285	arg1	release					258:264	extended release	249:264	extended release of diclofenac sodium using 32 full factorial design	249:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	0	11	theme	full	114:117	arg1	design					129:134	32 full factorial design	111:134	32 full factorial design	111:134	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	4	12	theme	drug	661:664	arg1	parameters					674:683	different drug release parameters	651:683	different drug release parameters	651:683	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	3	13	theme	entrapment	540:549	arg1	DEE					563:565	DEE	563:565	DEE	563:565	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	13	theme	entrapment	540:549	arg1	efficiency					551:560	drug entrapment efficiency	535:560	drug entrapment efficiency (DEE)	535:566	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	5	14	theme	h-Pmaa-g-GG	932:942	arg1	TSG					944:946	h-Pmaa-g-GG:TSG	932:946	h-Pmaa-g-GG:TSG	932:946	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	4	15	theme	Design-Expert	747:759	arg1	software					761:768	Design-Expert software	747:768	Design-Expert software	747:768	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	7	16	theme	case-I-Fickian	1166:1179	arg1	mechanism					1210:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
29567498	6	17	theme	extended	1074:1081	arg1	profile					1096:1102	an extended drug release profile	1071:1102	an extended drug release profile	1071:1102	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	5	18	from	decrease	907:914	arg1	ratio					923:927	the ratio	919:927	the ratio of h-Pmaa-g-GG:TSG	919:946	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	5	18	from	decrease	907:914	arg1	concentration					968:980	the concentration	964:980	the concentration of Ca2+ ions in cross-linking medium	964:1017	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	4	19	theme	different	651:659	arg1	parameters					674:683	different drug release parameters	651:683	different drug release parameters	651:683	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	5	20	theme	release	867:873	arg1	rate					875:878	Drug release rate	862:878	Drug release rate	862:878	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	0	21	theme	factorial	119:127	arg1	design					129:134	32 full factorial design	111:134	32 full factorial design	111:134	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	4	22	theme	release	845:851	arg1	profile					853:859	USP-reference release profile	831:859	USP-reference release profile	831:859	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	3	23	theme	drug	535:538	arg1	DEE					563:565	DEE	563:565	DEE	563:565	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	23	theme	drug	535:538	arg1	efficiency					551:560	drug entrapment efficiency	535:560	drug entrapment efficiency (DEE)	535:566	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	1	24	theme	-hydrolyzed	175:185	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	2	25	theme	each	491:494	arg1	levels					481:486	three different levels	465:486	three different levels of each	465:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	4	26	theme	factorial	724:732	arg1	design					734:739	32 full factorial design	716:739	32 full factorial design using Design-Expert software	716:768	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	1	27	theme	polymethacrylamide-g-gellan	187:213	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	0	28	theme	Tamarind	0:7	arg1	seed					9:12	Tamarind seed	0:12	Tamarind seed	0:12	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	1	29	theme	full	296:299	arg1	design					311:316	32 full factorial design	293:316	32 full factorial design	293:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	3	30	from	Effects	497:503	arg1	DEE					563:565	DEE	563:565	DEE	563:565	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	30	from	Effects	497:503	arg1	efficiency					551:560	drug entrapment efficiency	535:560	drug entrapment efficiency (DEE)	535:566	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	30	from	Effects	497:503	arg1	swelling					597:604	swelling	597:604	swelling	597:604	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	30	from	Effects	497:503	arg1	release					574:580	drug release	569:580	drug release	569:580	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	30	from	Effects	497:503	arg1	size					588:591	bead size	583:591	bead size	583:591	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	4	31	theme	full	719:722	arg1	design					734:739	32 full factorial design	716:739	32 full factorial design using Design-Expert software	716:768	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	2	32	theme	TSG	382:384	arg1	factors					452:458	independent factors	440:458	independent factors with three different levels of each	440:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	2	32	theme	TSG	382:384	arg1	ratio					357:361	The ratio	353:361	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2	353:424	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	0	33	theme	polymethacrylamide-g-gellan	29:55	arg1	beads					57:61	polymethacrylamide-g-gellan beads	29:61	polymethacrylamide-g-gellan beads for extended release of diclofenac sodium	29:103	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	1	34	theme	factorial	301:309	arg1	design					311:316	32 full factorial design	293:316	32 full factorial design	293:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	4	35	theme	USP-reference	831:843	arg1	profile					853:859	USP-reference release profile	831:859	USP-reference release profile	831:859	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	1	36	theme	h-Pmaa-g-GG	216:226	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	3	37	theme	CaCl2	526:530	arg1	Effects					497:503	Effects	497:503	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling	497:604	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	3	38	theme	ratio	516:520	arg1	Effects					497:503	Effects	497:503	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling	497:604	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	4	39	theme	release	666:672	arg1	parameters					674:683	different drug release parameters	651:683	different drug release parameters	651:683	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	7	40	theme	based	1191:1195	arg1	mechanism					1210:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
29567498	2	41	theme	CaCl2	420:424	arg1	concentration					390:402	concentration	390:402	concentration	390:402	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	2	41	theme	CaCl2	420:424	arg1	TSG					382:384	TSG	382:384	TSG	382:384	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	2	41	theme	CaCl2	420:424	arg1	h-Pmaa-g-GG					366:376	h-Pmaa-g-GG	366:376	h-Pmaa-g-GG	366:376	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	5	42	theme	cross-linking	998:1010	arg1	medium					1012:1017	cross-linking medium	998:1017	cross-linking medium	998:1017	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	43	theme	composite	229:237	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	2	44	theme	independent	440:450	arg1	factors					452:458	independent factors	440:458	independent factors with three different levels of each	440:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	2	44	theme	independent	440:450	arg1	ratio					357:361	The ratio	353:361	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2	353:424	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	5	45	theme	Drug	862:865	arg1	rate					875:878	Drug release rate	862:878	Drug release rate	862:878	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	46	theme	beads	239:243	arg1	purpose					330:336	the main purpose	321:336	the main purpose of this study	321:350	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	1	46	theme	beads	239:243	arg1	Development					137:147	Development	137:147	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design	137:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	2	47	theme	different	471:479	arg1	levels					481:486	three different levels	465:486	three different levels of each	465:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	1	48	theme	main	325:328	arg1	purpose					330:336	the main purpose	321:336	the main purpose of this study	321:350	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	1	48	theme	main	325:328	arg1	Development					137:147	Development	137:147	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design	137:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	6	49	theme	optimized	1024:1032	arg1	formulation					1034:1044	The optimized formulation	1020:1044	The optimized formulation	1020:1044	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	3	50	theme	drug	569:572	arg1	release					574:580	drug release	569:580	drug release	569:580	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	6	51	theme	release	1088:1094	arg1	profile					1096:1102	an extended drug release profile	1071:1102	an extended drug release profile	1071:1102	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	0	52	theme	extended	67:74	arg1	release					76:82	extended release	67:82	extended release of diclofenac sodium	67:103	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	7	53	theme	diffusion	1181:1189	arg1	mechanism					1210:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
29567498	6	54	theme	10h	1121:1123	arg1	period					1111:1116	a period	1109:1116	a period of 10h with f2=80.13	1109:1137	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	2	55	theme	h-Pmaa-g-GG	366:376	arg1	factors					452:458	independent factors	440:458	independent factors with three different levels of each	440:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	2	55	theme	h-Pmaa-g-GG	366:376	arg1	ratio					357:361	The ratio	353:361	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2	353:424	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	4	56	theme	formulation	786:796	arg1	factors					798:804	the formulation factors	782:804	the formulation factors	782:804	Responses such as DEE and different drug release parameters were statistically analyzed by 32 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile.
29567498	3	57	theme	bead	583:586	arg1	size					588:591	bead size	583:591	bead size	583:591	Effects of polymer ratio and CaCl2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated.
29567498	2	58	with	factors	452:458	arg1	levels					481:486	three different levels	465:486	three different levels of each	465:494	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	5	59	theme	TSG	944:946	arg1	ratio					923:927	the ratio	919:927	the ratio of h-Pmaa-g-GG:TSG	919:946	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	5	60	theme	Ca2+	985:988	arg1	ions					990:993	Ca2+ ions	985:993	Ca2+ ions	985:993	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	61	theme	tamarind	152:159	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	1	62	theme	extended	249:256	arg1	release					258:264	extended release	249:264	extended release of diclofenac sodium using 32 full factorial design	249:316	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	0	63	theme	diclofenac	87:96	arg1	sodium					98:103	diclofenac sodium	87:103	diclofenac sodium	87:103	Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.
29567498	2	64	theme	cross-linker	407:418	arg1	CaCl2					420:424	cross-linker CaCl2	407:424	cross-linker CaCl2	407:424	The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl2 were taken as independent factors with three different levels of each.
29567498	5	65	theme	ions	990:993	arg1	concentration					968:980	the concentration	964:980	the concentration of Ca2+ ions in cross-linking medium	964:1017	Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca2+ ions in cross-linking medium.
29567498	1	66	theme	seed	161:164	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	6	67	theme	%	1065:1065	arg1	DEE					1053:1055	DEE	1053:1055	DEE of 93.25%	1053:1065	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	6	67	theme	%	1065:1065	arg1	profile					1096:1102	an extended drug release profile	1071:1102	an extended drug release profile	1071:1102	The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f2=80.13.
29567498	7	68	theme	Kinetic	1140:1146	arg1	modeling					1148:1155	Kinetic modeling	1140:1155	Kinetic modeling	1140:1155	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
29567498	1	69	theme	gum	166:168	arg1	beads					239:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads	152:243	Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 32 full factorial design is the main purpose of this study.
29567498	7	70	theme	drug	1197:1200	arg1	mechanism					1210:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	case-I-Fickian diffusion based drug release mechanism	1166:1218	Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism.
31728768	0	0	theme	Chemical	61:68	arg1	Composition					70:80	Chemical Composition	61:80	Chemical Composition	61:80	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	7	1	theme	bamboo	1373:1378	arg1	samples					1380:1386	bamboo samples	1373:1386	bamboo samples	1373:1386	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	9	2	theme	bubbly	1627:1632	arg1	protuberance					1634:1645	uniformly distributed bubbly protuberance	1605:1645	uniformly distributed bubbly protuberance	1605:1645	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	1	3	theme	liquid	127:132	arg1	water					138:142	liquid hot water	127:142	liquid hot water combined with 1, 4-butanediol (LHW-BDO)	127:182	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	5	4	theme	intermolecular	864:877	arg1	bonds					888:892	intermolecular hydrogen bonds	864:892	intermolecular hydrogen bonds	864:892	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	5	theme	hemicellulose	1035:1047	arg1	separation					1010:1019	separation	1010:1019	separation	1010:1019	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	5	theme	hemicellulose	1035:1047	arg1	depolymerization					989:1004	depolymerization	989:1004	depolymerization	989:1004	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	6	6	theme	glucose	1156:1162	arg1	molecule					1164:1171	the glucose molecule	1152:1171	the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose	1152:1255	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	1	7	theme	hot	134:136	arg1	water					138:142	liquid hot water	127:142	liquid hot water combined with 1, 4-butanediol (LHW-BDO)	127:182	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	5	8	theme	aromatic	916:923	arg1	bonds					931:935	aromatic ether bonds	916:935	aromatic ether bonds of the fibers	916:949	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	6	9	theme	fiber	1184:1188	arg1	beneficial					1215:1224	beneficial	1215:1224	beneficial	1215:1224	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	6	9	theme	fiber	1184:1188	arg1	molecule					1190:1197	the fiber molecule	1180:1197	the fiber molecule	1180:1197	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	4	10	theme	removal	747:753	arg1	rate					755:758	the hemicellulose removal rate	729:758	the hemicellulose removal rate	729:758	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	3	11	theme	LHW-BDO	492:498	arg1	methods					513:519	LHW-BDO pretreatment methods	492:519	LHW-BDO pretreatment methods	492:519	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	4	12	from	content	615:621	arg1	residue					632:638	solid residue	626:638	solid residue	626:638	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	7	13	theme	fibers	1300:1305	arg1	zone					1285:1288	the amorphous zone	1271:1288	the amorphous zone of bamboo fibers	1271:1305	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	6	14	theme	LHW-BDO	1081:1087	arg1	pretreatment					1089:1100	LHW-BDO pretreatment	1081:1100	LHW-BDO pretreatment	1081:1100	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	1	15	from	effects	116:122	arg1	structure					216:224	structure	216:224	structure	216:224	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	1	15	from	effects	116:122	arg1	composition					200:210	chemical composition	191:210	chemical composition	191:210	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	3	16	theme	pretreatment	500:511	arg1	methods					513:519	LHW-BDO pretreatment methods	492:519	LHW-BDO pretreatment methods	492:519	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	4	17	theme	LHW-BDO	646:652	arg1	pretreatment					654:665	LHW-BDO pretreatment	646:665	LHW-BDO pretreatment	646:665	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	1	18	theme	water	138:142	arg1	effects					116:122	The effects	112:122	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo	112:239	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	6	19	theme	cellulose	1247:1255	arg1	separation					1233:1242	the separation	1229:1242	the separation of cellulose	1229:1255	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	5	20	theme	LHW-BDO	807:813	arg1	pretreatment					815:826	LHW-BDO pretreatment	807:826	LHW-BDO pretreatment	807:826	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	4	21	theme	recovery	698:705	arg1	rate					707:710	a recovery rate	696:710	a recovery rate of 75.68%	696:720	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	2	22	theme	X-ray	349:353	arg1	diffraction					355:365	X-ray diffraction	349:365	X-ray diffraction	349:365	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	8	23	theme	bamboo	1444:1449	arg1	crystallinity					1427:1439	The crystallinity	1423:1439	The crystallinity of bamboo pretreated with LHW-BDO	1423:1473	The crystallinity of bamboo pretreated with LHW-BDO was increased by 32.15%.
31728768	5	24	dep	depolymerization	989:1004	arg1	the					985:987	the	985:987	the	985:987	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	25	theme	hydrogen	848:855	arg1	bonds					857:861	the intramolecular hydrogen bonds	829:861	the intramolecular hydrogen bonds	829:861	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	4	26	theme	cellulose	605:613	arg1	content					615:621	the cellulose content	601:621	the cellulose content in solid residue after LHW-BDO pretreatment	601:665	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	7	27	theme	fiber	1354:1358	arg1	structure					1360:1368	the fiber structure	1350:1368	the fiber structure of bamboo samples	1350:1386	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	1	28	theme	moso	229:232	arg1	bamboo					234:239	moso bamboo	229:239	moso bamboo	229:239	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	2	29	theme	structure	264:272	arg1	changes					274:280	The structure changes	260:280	The structure changes of moso bamboo fibers	260:302	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	9	30	theme	scanning	1519:1526	arg1	microscopy					1537:1546	scanning electron microscopy	1519:1546	scanning electron microscopy	1519:1546	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	0	31	theme	Hot	18:20	arg1	Water					22:26	Liquid Hot Water	11:26	Liquid Hot Water	11:26	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	1	32	theme	bamboo	234:239	arg1	structure					216:224	structure	216:224	structure	216:224	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	1	32	theme	bamboo	234:239	arg1	composition					200:210	chemical composition	191:210	chemical composition	191:210	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	0	33	theme	Bamboo	104:109	arg1	Structure					86:94	Structure	86:94	Structure	86:94	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	0	33	theme	Bamboo	104:109	arg1	Composition					70:80	Chemical Composition	61:80	Chemical Composition	61:80	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	6	34	theme	β-glycoside	1123:1133	arg1	bond					1135:1138	the β-glycoside bond	1119:1138	the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose	1119:1255	However, LHW-BDO pretreatment does not destroy the β-glycoside bond which links the glucose molecule inside the fiber molecule, which was also beneficial to the separation of cellulose.
31728768	7	35	theme	amorphous	1275:1283	arg1	zone					1285:1288	the amorphous zone	1271:1288	the amorphous zone of bamboo fibers	1271:1305	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	2	36	theme	scanning	383:390	arg1	microscopy					401:410	electronic scanning electron microscopy	372:410	electronic scanning electron microscopy	372:410	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	2	37	theme	electron	392:399	arg1	microscopy					401:410	electronic scanning electron microscopy	372:410	electronic scanning electron microscopy	372:410	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	0	38	theme	Moso	99:102	arg1	Bamboo					104:109	Moso Bamboo	99:109	Moso Bamboo	99:109	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	4	39	theme	solid	626:630	arg1	residue					632:638	solid residue	626:638	solid residue	626:638	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	2	40	theme	electronic	372:381	arg1	microscopy					401:410	electronic scanning electron microscopy	372:410	electronic scanning electron microscopy	372:410	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	9	41	theme	electron	1528:1535	arg1	microscopy					1537:1546	scanning electron microscopy	1519:1546	scanning electron microscopy	1519:1546	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	3	42	theme	1	466:466	arg1	4-butanediol					469:480	4-butanediol	469:480	4-butanediol (BDO)	469:486	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	3	42	theme	1	466:466	arg1	rates					457:461	the delignification rates	437:461	the delignification rates of 1	437:466	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	3	42	theme	1	466:466	arg1	methods					513:519	LHW-BDO pretreatment methods	492:519	LHW-BDO pretreatment methods	492:519	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	3	42	theme	1	466:466	arg1	BDO					483:485	BDO	483:485	BDO	483:485	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	9	43	theme	bamboo	1583:1588	arg1	samples					1590:1596	the pretreated bamboo samples	1568:1596	the pretreated bamboo samples	1568:1596	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	5	44	theme	ether	925:929	arg1	bonds					931:935	aromatic ether bonds	916:935	aromatic ether bonds of the fibers	916:949	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	4	45	theme	BDO	583:585	arg1	pretreatment					587:598	BDO pretreatment	583:598	BDO pretreatment	583:598	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	0	46	theme	Water	22:26	arg1	Effects					0:6	Effects	0:6	Effects of Liquid Hot Water	0:26	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	9	47	theme	samples	1590:1596	arg1	surface					1557:1563	the surface	1553:1563	the surface of the pretreated bamboo samples	1553:1596	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	0	48	from	4-Butanediol	45:56	arg1	Structure					86:94	Structure	86:94	Structure	86:94	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	0	48	from	4-Butanediol	45:56	arg1	Composition					70:80	Chemical Composition	61:80	Chemical Composition	61:80	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	5	49	theme	lignin	1054:1059	arg1	molecules					1061:1069	lignin molecules	1054:1069	lignin molecules	1054:1069	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	3	50	theme	same	533:536	arg1	level					538:542	the same level	529:542	the same level	529:542	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	2	51	theme	fibers	297:302	arg1	changes					274:280	The structure changes	260:280	The structure changes of moso bamboo fibers	260:302	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	5	52	theme	molecules	1061:1069	arg1	separation					1010:1019	separation	1010:1019	separation	1010:1019	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	52	theme	molecules	1061:1069	arg1	depolymerization					989:1004	depolymerization	989:1004	depolymerization	989:1004	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	53	theme	fibers	944:949	arg1	bonds					888:892	intermolecular hydrogen bonds	864:892	intermolecular hydrogen bonds	864:892	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	53	theme	fibers	944:949	arg1	bonds					905:909	methylene bonds	895:909	methylene bonds	895:909	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	53	theme	fibers	944:949	arg1	bonds					857:861	the intramolecular hydrogen bonds	829:861	the intramolecular hydrogen bonds	829:861	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	53	theme	fibers	944:949	arg1	bonds					931:935	aromatic ether bonds	916:935	aromatic ether bonds of the fibers	916:949	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	4	54	theme	hemicellulose	733:745	arg1	rate					755:758	the hemicellulose removal rate	729:758	the hemicellulose removal rate	729:758	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	2	55	theme	bamboo	290:295	arg1	fibers					297:302	moso bamboo fibers	285:302	moso bamboo fibers	285:302	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	4	56	theme	%	720:720	arg1	rate					707:710	a recovery rate	696:710	a recovery rate of 75.68%	696:720	However, compared with BDO pretreatment, the cellulose content in solid residue after LHW-BDO pretreatment was increased by 17.06% with a recovery rate of 75.68%, while the hemicellulose removal rate increased by 115.33% and reached 50.34%.
31728768	9	57	theme	distributed	1615:1625	arg1	protuberance					1634:1645	uniformly distributed bubbly protuberance	1605:1645	uniformly distributed bubbly protuberance	1605:1645	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	7	58	theme	samples	1380:1386	arg1	structure					1360:1368	the fiber structure	1350:1368	the fiber structure of bamboo samples	1350:1386	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	2	59	theme	moso	285:288	arg1	fibers					297:302	moso bamboo fibers	285:302	moso bamboo fibers	285:302	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	7	60	theme	bamboo	1293:1298	arg1	fibers					1300:1305	bamboo fibers	1293:1305	bamboo fibers	1293:1305	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	5	61	theme	methylene	895:903	arg1	bonds					905:909	methylene bonds	895:909	methylene bonds	895:909	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	1	62	dep	composition	200:210	arg1	the					187:189	the	187:189	the	187:189	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
31728768	5	63	theme	hydrogen	879:886	arg1	bonds					888:892	intermolecular hydrogen bonds	864:892	intermolecular hydrogen bonds	864:892	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	64	theme	intramolecular	833:846	arg1	bonds					857:861	the intramolecular hydrogen bonds	829:861	the intramolecular hydrogen bonds	829:861	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	3	65	theme	delignification	441:455	arg1	rates					457:461	the delignification rates	437:461	the delignification rates of 1	437:466	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	7	66	theme	above	1328:1332	arg1	treatments					1334:1343	the above treatments	1324:1343	the above treatments	1324:1343	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	2	67	theme	infrared	326:333	arg1	spectroscopy					335:346	infrared spectroscopy	326:346	infrared spectroscopy	326:346	The structure changes of moso bamboo fibers were characterized by infrared spectroscopy, X-ray diffraction, and electronic scanning electron microscopy.
31728768	3	68	dep	level	538:542	arg1	%					556:556	91.42-93.08%	545:556	91.42-93.08%	545:556	The results showed that the delignification rates of 1, 4-butanediol (BDO) and LHW-BDO pretreatment methods were at the same level (91.42-93.08%).
31728768	5	69	theme	cellulose	1024:1032	arg1	separation					1010:1019	separation	1010:1019	separation	1010:1019	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	5	69	theme	cellulose	1024:1032	arg1	depolymerization					989:1004	depolymerization	989:1004	depolymerization	989:1004	After LHW-BDO pretreatment, the intramolecular hydrogen bonds, intermolecular hydrogen bonds, methylene bonds, and aromatic ether bonds of the fibers were broken, which contributed to the depolymerization and separation of cellulose, hemicellulose, and lignin molecules.
31728768	0	70	theme	Liquid	11:16	arg1	Water					22:26	Liquid Hot Water	11:26	Liquid Hot Water	11:26	Effects of Liquid Hot Water Combined with 1, 4-Butanediol on Chemical Composition and Structure of Moso Bamboo.
31728768	7	71	theme	crystalline	1405:1415	arg1	form					1417:1420	crystalline form	1405:1420	crystalline form	1405:1420	In addition, the amorphous zone of bamboo fibers was destroyed by the above treatments, and the fiber structure of bamboo samples mostly exists in crystalline form.
31728768	9	72	theme	pretreated	1572:1581	arg1	samples					1590:1596	the pretreated bamboo samples	1568:1596	the pretreated bamboo samples	1568:1596	It can be found by scanning electron microscopy that the surface of the pretreated bamboo samples showed uniformly distributed bubbly protuberance.
31728768	1	73	theme	chemical	191:198	arg1	composition					200:210	chemical composition	191:210	chemical composition	191:210	The effects of liquid hot water combined with 1, 4-butanediol (LHW-BDO) on the chemical composition and structure of moso bamboo were investigated.
30825510	12	0	theme	stereomicroscopic	1883:1899	arg1	evaluation					1906:1915	stereomicroscopic data evaluation	1883:1915	stereomicroscopic data evaluation	1883:1915	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	6	1	theme	polyethylene	1005:1016	arg1	glycol					1018:1023	osmotically active polyethylene glycol	986:1023	osmotically active polyethylene glycol (PEG)	986:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	1	theme	polyethylene	1005:1016	arg1	PEG					1026:1028	PEG	1026:1028	PEG	1026:1028	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	10	2	theme	sample	1428:1433	arg1	stability					1435:1443	The sample stability	1424:1443	The sample stability	1424:1443	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	3	3	theme	wet	457:459	arg1	WT					472:473	WT	472:473	WT	472:473	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	3	3	theme	wet	457:459	arg1	treatment					461:469	wet treatment	457:469	wet treatment (WT)	457:474	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	9	4	theme	pellet	1316:1321	arg1	coating					1323:1329	The pellet coating	1312:1329	The pellet coating	1312:1329	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	6	5	theme	submitted	798:806	arg1	work					808:811	The submitted work	794:811	The submitted work	794:811	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	2	6	theme	Dry	271:273	arg1	prevention					330:339	a prevention	328:339	a prevention of this process	328:355	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	6	theme	Dry	271:273	arg1	DT					294:295	DT	294:295	DT	294:295	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	6	theme	Dry	271:273	arg1	treatment					283:291	Dry thermal treatment	271:291	Dry thermal treatment (DT) of the coating	271:311	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	13	7	theme	substance	2015:2023	arg1	penetration					2025:2035	the soluble substance penetration	2003:2035	the soluble substance penetration through the coating in the case of PEG pellets	2003:2082	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	12	8	theme	elevated	1820:1827	arg1	humidity					1829:1836	elevated humidity	1820:1836	elevated humidity	1820:1836	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	11	9	theme	glycol	1681:1686	arg1	penetration					1641:1651	the penetration	1637:1651	the penetration of glucose and polyethylene glycol	1637:1686	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	3	10	theme	film	513:516	arg1	formation					518:526	the film formation	509:526	the film formation	509:526	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	6	11	theme	pellet	857:862	arg1	compositions					869:880	two pellet core compositions	853:880	two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	853:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	10	12	theme	core	1483:1486	arg1	composition					1488:1498	the pellet core composition	1472:1498	the pellet core composition (PEG, CMS)	1472:1509	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	13	13	theme	stress	1977:1982	arg1	tests					1984:1988	the stress tests	1973:1988	the stress tests	1973:1988	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	13	14	theme	pellets	2137:2143	arg1	case					2125:2128	the case	2121:2128	the case of CMS pellets	2121:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	15	15	from	stable	2342:2347	arg1	environment					2356:2366	the environment	2352:2366	the environment	2352:2366	The aforementioned processes do not occur after DT and the pellets are stable in the environment without increased humidity.
30825510	6	16	theme	stability	832:840	arg1	testing					842:848	the stability testing	828:848	the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	828:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	13	17	theme	dissolution	1949:1959	arg1	rate					1961:1964	dissolution rate	1949:1964	dissolution rate	1949:1964	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	9	18	theme	lag	1400:1402	arg1	time					1404:1407	the required lag time	1387:1407	the required lag time (120-360 min)	1387:1421	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	9	18	theme	lag	1400:1402	arg1	120-360 min					1410:1420	120-360 min	1410:1420	120-360 min	1410:1420	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	4	19	theme	water	645:649	arg1	penetration					630:640	the penetration	626:640	the penetration of water into the core causing the increase in the system volume	626:705	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	1	20	theme	particle	123:130	arg1	coalescence					132:142	the additional particle coalescence	108:142	the additional particle coalescence in the coating	108:157	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	6	21	theme	sodium	929:934	arg1	CMS					958:960	CMS	958:960	CMS	958:960	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	21	theme	sodium	929:934	arg1	starch					950:955	swelling superdisintegrant sodium carboxymethyl starch	902:955	swelling superdisintegrant sodium carboxymethyl starch (CMS)	902:961	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	12	22	theme	evaluation	1906:1915	arg1	means					1874:1878	the means	1870:1878	the means of stereomicroscopic data evaluation	1870:1915	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	14	23	theme	observed	2150:2157	arg1	mechanisms					2159:2168	The observed mechanisms	2146:2168	The observed mechanisms	2146:2168	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
30825510	0	24	theme	dissolution	47:57	arg1	profile					59:65	the dissolution profile	43:65	the dissolution profile of ethylcellulose	43:83	The effects of the treatment conditions on the dissolution profile of ethylcellulose coated pellets.
30825510	15	25	theme	aforementioned	2275:2288	arg1	processes					2290:2298	The aforementioned processes	2271:2298	The aforementioned processes	2271:2298	The aforementioned processes do not occur after DT and the pellets are stable in the environment without increased humidity.
30825510	13	26	theme	form	2097:2100	arg1	increase					2109:2116	dosage form volume increase	2090:2116	dosage form volume increase in the case of CMS pellets	2090:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	6	27	theme	swelling	902:909	arg1	CMS					958:960	CMS	958:960	CMS	958:960	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	27	theme	swelling	902:909	arg1	starch					950:955	swelling superdisintegrant sodium carboxymethyl starch	902:955	swelling superdisintegrant sodium carboxymethyl starch (CMS)	902:961	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	28	dep	compositions	869:880	arg1	pellets					883:889	pellets	883:889	two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	853:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	12	29	theme	pellet	1770:1775	arg1	swelling					1777:1784	significant pellet swelling	1758:1784	significant pellet swelling after WT (under the conditions of elevated humidity)	1758:1837	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	15	30	theme	increased	2376:2384	arg1	humidity					2386:2393	increased humidity	2376:2393	increased humidity	2376:2393	The aforementioned processes do not occur after DT and the pellets are stable in the environment without increased humidity.
30825510	2	31	used	used	320:323	arg2	prevention					330:339	a prevention	328:339	a prevention of this process	328:355	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	31	used	used	320:323	arg2	DT					294:295	DT	294:295	DT	294:295	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	31	used	used	320:323	arg2	treatment					283:291	Dry thermal treatment	271:291	Dry thermal treatment (DT) of the coating	271:311	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	8	32	theme	pellet	1268:1273	arg1	cores					1275:1279	pellet cores	1268:1279	pellet cores	1268:1279	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	7	33	theme	dissolution	1120:1130	arg1	profiles					1132:1139	the pellet dissolution profiles	1109:1139	the pellet dissolution profiles	1109:1139	Another objective was to identify the treatment/storage condition effects on the pellet dissolution profiles.
30825510	1	34	from	coalescence	132:142	arg1	coating					151:157	the coating	147:157	the coating	147:157	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	6	35	theme	compositions	869:880	arg1	testing					842:848	the stability testing	828:848	the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	828:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	2	36	theme	process	349:355	arg1	prevention					330:339	a prevention	328:339	a prevention of this process	328:355	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	36	theme	process	349:355	arg1	treatment					283:291	Dry thermal treatment	271:291	Dry thermal treatment (DT) of the coating	271:311	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	1	37	theme	ethylcellulose	249:262	arg1	latex					264:268	aqueous ethylcellulose latex	241:268	aqueous ethylcellulose latex	241:268	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	12	38	theme	CMS	1746:1748	arg1	sample					1750:1755	the CMS sample	1742:1755	the CMS sample	1742:1755	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	7	39	from	effects	1098:1104	arg1	profiles					1132:1139	the pellet dissolution profiles	1109:1139	the pellet dissolution profiles	1109:1139	Another objective was to identify the treatment/storage condition effects on the pellet dissolution profiles.
30825510	13	40	theme	PEG	2072:2074	arg1	pellets					2076:2082	PEG pellets	2072:2082	PEG pellets	2072:2082	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	7	41	theme	treatment/storage	1070:1086	arg1	effects					1098:1104	the treatment/storage condition effects	1066:1104	the treatment/storage condition effects on the pellet dissolution profiles	1066:1139	Another objective was to identify the treatment/storage condition effects on the pellet dissolution profiles.
30825510	15	42	from	environment	2356:2366	arg1	stable					2342:2347	stable	2342:2347	stable	2342:2347	The aforementioned processes do not occur after DT and the pellets are stable in the environment without increased humidity.
30825510	15	42	from	environment	2356:2366	arg1	pellets					2330:2336	the pellets	2326:2336	the pellets	2326:2336	The aforementioned processes do not occur after DT and the pellets are stable in the environment without increased humidity.
30825510	0	43	theme	treatment	19:27	arg1	conditions					29:38	the treatment conditions	15:38	the treatment conditions	15:38	The effects of the treatment conditions on the dissolution profile of ethylcellulose coated pellets.
30825510	3	44	theme	high	436:439	arg1	humidity					441:448	high humidity	436:448	high humidity	436:448	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	13	45	from	penetration	2025:2035	arg1	case					2064:2067	the case	2060:2067	the case of PEG pellets	2060:2082	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	11	46	theme	PEG	1709:1711	arg1	surface					1720:1726	the PEG pellet surface	1705:1726	the PEG pellet surface	1705:1726	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	4	47	theme	coating	603:609	arg1	rupture					611:617	the coating rupture	599:617	the coating rupture due to the penetration of water into the core causing the increase in the system volume	599:705	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	11	48	theme	Scanning	1571:1578	arg1	microscopy					1589:1598	Scanning electron microscopy	1571:1598	Scanning electron microscopy	1571:1598	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	14	49	theme	dosage	2257:2262	arg1	forms					2264:2268	the ethylcellulose coated dosage forms	2231:2268	the ethylcellulose coated dosage forms	2231:2268	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
30825510	14	50	theme	ethylcellulose	2235:2248	arg1	forms					2264:2268	the ethylcellulose coated dosage forms	2231:2268	the ethylcellulose coated dosage forms	2231:2268	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
30825510	6	51	theme	active	998:1003	arg1	glycol					1018:1023	osmotically active polyethylene glycol	986:1023	osmotically active polyethylene glycol (PEG)	986:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	51	theme	active	998:1003	arg1	PEG					1026:1028	PEG	1026:1028	PEG	1026:1028	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	10	52	theme	pellet	1476:1481	arg1	composition					1488:1498	the pellet core composition	1472:1498	the pellet core composition (PEG, CMS)	1472:1509	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	13	53	from	increase	2109:2116	arg1	case					2125:2128	the case	2121:2128	the case of CMS pellets	2121:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	2	54	theme	thermal	275:281	arg1	prevention					330:339	a prevention	328:339	a prevention of this process	328:355	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	54	theme	thermal	275:281	arg1	DT					294:295	DT	294:295	DT	294:295	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	54	theme	thermal	275:281	arg1	treatment					283:291	Dry thermal treatment	271:291	Dry thermal treatment (DT) of the coating	271:311	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	1	55	theme	aqueous	241:247	arg1	latex					264:268	aqueous ethylcellulose latex	241:268	aqueous ethylcellulose latex	241:268	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	8	56	with	patients	1197:1204	arg1	mellitus					1220:1227	diabetes mellitus	1211:1227	diabetes mellitus	1211:1227	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	10	57	dep	composition	1488:1498	arg1	CMS					1506:1508	CMS	1506:1508	CMS	1506:1508	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	10	57	dep	composition	1488:1498	arg1	PEG					1501:1503	PEG	1501:1503	PEG	1501:1503	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	14	58	theme	stability	2210:2218	arg1	testing					2220:2226	the stability testing	2206:2226	the stability testing of the ethylcellulose coated dosage forms	2206:2268	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
30825510	9	59	theme	ethylcellulose-based	1344:1363	arg1	latex					1365:1369	ethylcellulose-based latex	1344:1369	ethylcellulose-based latex	1344:1369	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	6	60	contain	containing	975:984	arg1	pellets					967:973	pellets	967:973	pellets containing osmotically active polyethylene glycol (PEG)	967:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	60	contain	containing	975:984	arg2	PEG					1026:1028	PEG	1026:1028	PEG	1026:1028	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	60	contain	containing	975:984	arg2	glycol					1018:1023	osmotically active polyethylene glycol	986:1023	osmotically active polyethylene glycol (PEG)	986:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	61	theme	core	864:867	arg1	compositions					869:880	two pellet core compositions	853:880	two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	853:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	8	62	theme	glucose	1303:1309	arg1	glucose					1303:1309	glucose	1303:1309	glucose	1303:1309	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	8	62	theme	glucose	1303:1309	arg1	%					1291:1291	75%	1289:1291	75%	1289:1291	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	8	62	theme	glucose	1303:1309	arg1	%					1298:1298	80%	1296:1298	80%	1296:1298	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	1	63	theme	dissolution	175:185	arg1	profile					187:193	the dissolution profile	171:193	the dissolution profile	171:193	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	12	64	theme	humidity	1829:1836	arg1	conditions					1806:1815	the conditions	1802:1815	the conditions of elevated humidity	1802:1836	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	4	65	theme	time-controlled	555:569	arg1	systems					571:577	time-controlled systems	555:577	time-controlled systems	555:577	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	2	66	theme	coating	305:311	arg1	prevention					330:339	a prevention	328:339	a prevention of this process	328:355	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	66	theme	coating	305:311	arg1	DT					294:295	DT	294:295	DT	294:295	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	2	66	theme	coating	305:311	arg1	treatment					283:291	Dry thermal treatment	271:291	Dry thermal treatment (DT) of the coating	271:311	Dry thermal treatment (DT) of the coating can be used as a prevention of this process.
30825510	9	67	theme	required	1391:1398	arg1	time					1404:1407	the required lag time	1387:1407	the required lag time (120-360 min)	1387:1421	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	9	67	theme	required	1391:1398	arg1	120-360 min					1410:1420	120-360 min	1410:1420	120-360 min	1410:1420	The pellet coating is formed by ethylcellulose-based latex, which provides the required lag time (120-360 min).
30825510	8	68	theme	diabetes	1211:1218	arg1	mellitus					1220:1227	diabetes mellitus	1211:1227	diabetes mellitus	1211:1227	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	13	69	theme	rate	1961:1964	arg1	acceleration					1933:1944	the acceleration	1929:1944	the acceleration of dissolution rate during the stress tests	1929:1988	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	1	70	theme	additional	112:121	arg1	coalescence					132:142	the additional particle coalescence	108:142	the additional particle coalescence in the coating	108:157	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	6	71	theme	carboxymethyl	936:948	arg1	CMS					958:960	CMS	958:960	CMS	958:960	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	71	theme	carboxymethyl	936:948	arg1	starch					950:955	swelling superdisintegrant sodium carboxymethyl starch	902:955	swelling superdisintegrant sodium carboxymethyl starch (CMS)	902:961	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	3	72	theme	effect	426:431	arg1	advantage					397:405	advantage	397:405	advantage of the synergistic effect of high humidity	397:448	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	13	73	theme	CMS	2133:2135	arg1	pellets					2137:2143	CMS pellets	2133:2143	CMS pellets	2133:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	13	74	theme	dosage	2090:2095	arg1	increase					2109:2116	dosage form volume increase	2090:2116	dosage form volume increase in the case of CMS pellets	2090:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	6	75	theme	superdisintegrant	911:927	arg1	CMS					958:960	CMS	958:960	CMS	958:960	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	75	theme	superdisintegrant	911:927	arg1	starch					950:955	swelling superdisintegrant sodium carboxymethyl starch	902:955	swelling superdisintegrant sodium carboxymethyl starch (CMS)	902:961	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	12	76	theme	data	1901:1904	arg1	evaluation					1906:1915	stereomicroscopic data evaluation	1883:1915	stereomicroscopic data evaluation	1883:1915	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	11	77	theme	Raman	1604:1608	arg1	microspectroscopy					1610:1626	Raman microspectroscopy	1604:1626	Raman microspectroscopy	1604:1626	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	0	78	theme	ethylcellulose	70:83	arg1	profile					59:65	the dissolution profile	43:65	the dissolution profile of ethylcellulose	43:83	The effects of the treatment conditions on the dissolution profile of ethylcellulose coated pellets.
30825510	1	79	from	changes	160:166	arg1	profile					187:193	the dissolution profile	171:193	the dissolution profile	171:193	Due to the additional particle coalescence in the coating, changes in the dissolution profile occur over time in the formulations coated by aqueous ethylcellulose latex.
30825510	13	80	theme	volume	2102:2107	arg1	increase					2109:2116	dosage form volume increase	2090:2116	dosage form volume increase in the case of CMS pellets	2090:2143	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	0	81	from	effects	4:10	arg1	profile					59:65	the dissolution profile	43:65	the dissolution profile of ethylcellulose	43:83	The effects of the treatment conditions on the dissolution profile of ethylcellulose coated pellets.
30825510	4	82	theme	due	619:621	arg1	rupture					611:617	the coating rupture	599:617	the coating rupture due to the penetration of water into the core causing the increase in the system volume	599:705	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	11	83	theme	glucose	1656:1662	arg1	penetration					1641:1651	the penetration	1637:1651	the penetration of glucose and polyethylene glycol	1637:1686	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	7	84	theme	pellet	1113:1118	arg1	profiles					1132:1139	the pellet dissolution profiles	1109:1139	the pellet dissolution profiles	1109:1139	Another objective was to identify the treatment/storage condition effects on the pellet dissolution profiles.
30825510	4	85	from	increase	677:684	arg1	volume					700:705	the system volume	689:705	the system volume	689:705	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	8	86	contain	contain	1281:1287	arg1	cores					1275:1279	pellet cores	1268:1279	pellet cores	1268:1279	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	8	86	contain	contain	1281:1287	arg2	%					1298:1298	80%	1296:1298	80%	1296:1298	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	8	86	contain	contain	1281:1287	arg2	%					1291:1291	75%	1289:1291	75%	1289:1291	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	8	86	contain	contain	1281:1287	arg2	glucose					1303:1309	glucose	1303:1309	glucose	1303:1309	These pellets are intended to prevent hypoglycemia for patients with diabetes mellitus and therefore, besides the excipients, pellet cores contain 75% or 80% of glucose.
30825510	11	87	dep	surface	1720:1726	arg1	core					1697:1700	core	1697:1700	core	1697:1700	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	11	87	dep	surface	1720:1726	arg1	the					1693:1695	the	1693:1695	the	1693:1695	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	7	88	theme	condition	1088:1096	arg1	effects					1098:1104	the treatment/storage condition effects	1066:1104	the treatment/storage condition effects on the pellet dissolution profiles	1066:1139	Another objective was to identify the treatment/storage condition effects on the pellet dissolution profiles.
30825510	11	89	theme	polyethylene	1668:1679	arg1	glycol					1681:1686	polyethylene glycol	1668:1686	polyethylene glycol	1668:1686	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	12	90	theme	significant	1758:1768	arg1	swelling					1777:1784	significant pellet swelling	1758:1784	significant pellet swelling after WT (under the conditions of elevated humidity)	1758:1837	For the CMS sample, significant pellet swelling after WT (under the conditions of elevated humidity) was statistically confirmed by the means of stereomicroscopic data evaluation.
30825510	13	91	theme	pellets	2076:2082	arg1	case					2064:2067	the case	2060:2067	the case of PEG pellets	2060:2082	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	6	92	contain	containing	891:900	arg1	pellets					883:889	pellets	883:889	two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG)	853:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	92	contain	containing	891:900	arg2	starch					950:955	swelling superdisintegrant sodium carboxymethyl starch	902:955	swelling superdisintegrant sodium carboxymethyl starch (CMS)	902:961	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	92	contain	containing	891:900	arg2	pellets					967:973	pellets	967:973	pellets containing osmotically active polyethylene glycol (PEG)	967:1029	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	6	92	contain	containing	891:900	arg2	CMS					958:960	CMS	958:960	CMS	958:960	The submitted work was focused on the stability testing of two pellet core compositions: pellets containing swelling superdisintegrant sodium carboxymethyl starch (CMS) and pellets containing osmotically active polyethylene glycol (PEG).
30825510	3	93	theme	synergistic	414:424	arg1	effect					426:431	the synergistic effect	410:431	the synergistic effect of high humidity	410:448	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	0	94	theme	conditions	29:38	arg1	effects					4:10	The effects	0:10	The effects of the treatment conditions on the dissolution profile of ethylcellulose	0:83	The effects of the treatment conditions on the dissolution profile of ethylcellulose coated pellets.
30825510	10	95	theme	final	1528:1532	arg1	pellet					1534:1539	final pellet	1528:1539	final pellet coating treatment (DT or WT)	1528:1568	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	10	96	theme	pellet	1534:1539	arg1	types					1519:1523	two types	1515:1523	two types of final pellet coating treatment (DT or WT)	1515:1568	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	11	97	theme	electron	1580:1587	arg1	microscopy					1589:1598	Scanning electron microscopy	1571:1598	Scanning electron microscopy	1571:1598	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	10	98	dep	treatment	1549:1557	arg1	WT					1566:1567	WT	1566:1567	WT	1566:1567	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	10	98	dep	treatment	1549:1557	arg1	DT					1560:1561	DT	1560:1561	DT	1560:1561	The sample stability was evaluated depending on the pellet core composition (PEG, CMS) for two types of final pellet coating treatment (DT or WT).
30825510	3	99	theme	humidity	441:448	arg1	effect					426:431	the synergistic effect	410:431	the synergistic effect of high humidity	410:448	Alternatively, it is advisable to take advantage of the synergistic effect of high humidity during wet treatment (WT), which substantially accelerates the film formation.
30825510	4	100	theme	system	693:698	arg1	volume					700:705	the system volume	689:705	the system volume	689:705	This can be a problem for time-controlled systems, which are based on the coating rupture due to the penetration of water into the core causing the increase in the system volume.
30825510	14	101	theme	forms	2264:2268	arg1	testing					2220:2226	the stability testing	2206:2226	the stability testing of the ethylcellulose coated dosage forms	2206:2268	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
30825510	11	102	theme	pellet	1713:1718	arg1	surface					1720:1726	the PEG pellet surface	1705:1726	the PEG pellet surface	1705:1726	Scanning electron microscopy and Raman microspectroscopy revealed the penetration of glucose and polyethylene glycol from the core to the PEG pellet surface after WT.
30825510	13	103	theme	soluble	2007:2013	arg1	penetration					2025:2035	the soluble substance penetration	2003:2035	the soluble substance penetration through the coating in the case of PEG pellets	2003:2082	Therefore, the acceleration of dissolution rate during the stress tests is caused by the soluble substance penetration through the coating in the case of PEG pellets or by dosage form volume increase in the case of CMS pellets.
30825510	14	104	theme	coated	2250:2255	arg1	forms					2264:2268	the ethylcellulose coated dosage forms	2231:2268	the ethylcellulose coated dosage forms	2231:2268	The observed mechanisms can be generally anticipated during the stability testing of the ethylcellulose coated dosage forms.
31930869	0	0	theme	Extract	91:97	arg1	Nanoliposomes					60:72	Nanoliposomes	60:72	Nanoliposomes of Mandarin Peel Extract	60:97	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	4	1	dep	concentrations	535:548	arg1	EE					608:609	the highest EE	596:609	the highest EE in processed cheese	596:629	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	7	2	theme	cheese	906:911	arg1	composition					867:877	The chemical composition	854:877	The chemical composition of the resultant processed cheese	854:911	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	4	3	dep	%	698:698	arg1	50					688:689	50	688:689	50	688:689	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	3	dep	%	698:698	arg1	100					695:697	100	695:697	100	695:697	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	7	4	theme	half	962:965	arg1	fat					967:969	half fat	962:969	half fat processed cheese	962:986	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	0	5	theme	Peel	86:89	arg1	Extract					91:97	Mandarin Peel Extract	77:97	Mandarin Peel Extract	77:97	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	6	6	theme	MPEP	821:824	arg1	nanoliposomes					826:838	MPEP nanoliposomes	821:838	MPEP nanoliposomes	821:838	Rustles: High EE (>80%) of MPEP nanoliposomes was achieved.
31930869	1	7	dep	BACKGROUND	100:109	arg1	fruit					138:142	The Mandarin fruit	125:142	The Mandarin fruit	125:142	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	1	7	dep	BACKGROUND	100:109	arg1	source					159:164	a wonderful source	147:164	a wonderful source of essential dietary nutrients	147:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	4	8	dep	MATERIALS	419:427	arg1	efficiency					459:468	The encapsulation efficiency	441:468	The encapsulation efficiency	441:468	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	7	9	theme	chemical	858:865	arg1	composition					867:877	The chemical composition	854:877	The chemical composition of the resultant processed cheese	854:911	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	7	10	theme	resultant	886:894	arg1	cheese					906:911	the resultant processed cheese	882:911	the resultant processed cheese	882:911	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	9	11	dep	CONCLUSION	1129:1138	arg1	remained					1227:1234	remained	1227:1234	remained unaffected during cold storage	1227:1265	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	4	12	theme	extract	492:498	arg1	powder					500:505	mandarin peel extract powder	478:505	mandarin peel extract powder (MPEP)	478:512	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	12	theme	extract	492:498	arg1	MPEP					508:511	MPEP	508:511	MPEP	508:511	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	10	13	theme	effective	1365:1373	arg1	vesicle					1384:1390	a simple and effective delivery vesicle	1352:1390	a simple and effective delivery vesicle for phenolic compounds	1352:1413	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	3	14	theme	cheese	354:359	arg1	production					319:328	production	319:328	production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract	319:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	3	14	theme	cheese	354:359	arg1	objective					291:299	The objective	287:299	The objective of this study	287:313	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	4	15	theme	peel	487:490	arg1	powder					500:505	mandarin peel extract powder	478:505	mandarin peel extract powder (MPEP)	478:512	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	15	theme	peel	487:490	arg1	MPEP					508:511	MPEP	508:511	MPEP	508:511	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	5	16	theme	phenolics	740:748	arg1	content					750:756	phenolics content	740:756	phenolics content	740:756	The physicochemical properties and phenolics content for processed cheese were analyzed.
31930869	1	17	theme	essential	169:177	arg1	nutrients					187:195	essential dietary nutrients	169:195	essential dietary nutrients	169:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	9	18	theme	MPEP	1208:1211	arg1	nanoliposomes					1213:1225	MPEP nanoliposomes	1208:1225	MPEP nanoliposomes	1208:1225	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	1	19	theme	dietary	179:185	arg1	nutrients					187:195	essential dietary nutrients	169:195	essential dietary nutrients	169:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	3	20	theme	peel	405:408	arg1	extract					410:416	mandarin peel extract	396:416	mandarin peel extract	396:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	4	21	theme	powder	500:505	arg1	MATERIALS					419:427	MATERIALS	419:427	MATERIALS	419:427	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	21	theme	powder	500:505	arg1	EE					471:472	EE	471:472	EE	471:472	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	21	theme	powder	500:505	arg1	METHODS					433:439	METHODS	433:439	METHODS	433:439	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	8	22	theme	Physical	989:996	arg1	properties					1015:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	3	23	theme	functional	333:342	arg1	cheese					354:359	functional processed cheese	333:359	functional processed cheese supplemented with nanoliposomes of mandarin peel extract	333:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	4	24	dep	ratios	677:682	arg1	v/v					700:702	25, 50 and 100% v/v	684:702	ratios 25, 50 and 100% v/v	677:702	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	25	from	EE	608:609	arg1	cheese					624:629	processed cheese	614:629	processed cheese	614:629	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	1	26	theme	nutrients	187:195	arg1	fruit					138:142	The Mandarin fruit	125:142	The Mandarin fruit	125:142	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	1	26	theme	nutrients	187:195	arg1	source					159:164	a wonderful source	147:164	a wonderful source of essential dietary nutrients	147:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	3	27	theme	extract	410:416	arg1	nanoliposomes					379:391	nanoliposomes	379:391	nanoliposomes of mandarin peel extract	379:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	8	28	theme	color	1030:1034	arg1	parameters					1036:1045	color parameters	1030:1045	color parameters	1030:1045	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	9	29	theme	processed	1165:1173	arg1	samples					1182:1188	processed cheese samples	1165:1188	processed cheese samples supplemented with MPEP nanoliposomes	1165:1225	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	2	30	from	phenolics	257:265	arg1	food					281:284	functional food	270:284	functional food	270:284	The liposome is an encapsulation method to incorporate the phenolics in functional food.
31930869	4	31	theme	processed	614:622	arg1	cheese					624:629	processed cheese	614:629	processed cheese	614:629	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	0	32	theme	Processed	25:33	arg1	Cheese					35:40	Functional Processed Cheese	14:40	Functional Processed Cheese	14:40	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	10	33	theme	phenolic	1396:1403	arg1	compounds					1405:1413	phenolic compounds	1396:1413	phenolic compounds	1396:1413	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	8	34	theme	organoleptic	1002:1013	arg1	properties					1015:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	9	35	theme	cheese	1175:1180	arg1	samples					1182:1188	processed cheese samples	1165:1188	processed cheese samples supplemented with MPEP nanoliposomes	1165:1225	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	10	36	dep	retained	1308:1315	arg1	presented					1342:1350	presented	1342:1350	presented a simple and effective delivery vesicle for phenolic compounds	1342:1413	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	3	37	theme	mandarin	396:403	arg1	extract					410:416	mandarin peel extract	396:416	mandarin peel extract	396:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	9	38	theme	samples	1182:1188	arg1	characterizations					1144:1160	The characterizations	1140:1160	The characterizations of processed cheese samples supplemented with MPEP nanoliposomes	1140:1225	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	6	39	theme	nanoliposomes	826:838	arg1	%					815:815	>80%	812:815	>80%	812:815	Rustles: High EE (>80%) of MPEP nanoliposomes was achieved.
31930869	6	39	theme	nanoliposomes	826:838	arg1	EE					808:809	High EE	803:809	High EE (>80%) of MPEP nanoliposomes	803:838	Rustles: High EE (>80%) of MPEP nanoliposomes was achieved.
31930869	4	40	theme	MPEP	655:658	arg1	nanoliposomes					660:672	MPEP nanoliposomes	655:672	MPEP nanoliposomes at ratios 25, 50 and 100% v/v	655:702	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	7	41	theme	processed	971:979	arg1	cheese					981:986	half fat processed cheese	962:986	half fat processed cheese	962:986	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	6	42	theme	High	803:806	arg1	%					815:815	>80%	812:815	>80%	812:815	Rustles: High EE (>80%) of MPEP nanoliposomes was achieved.
31930869	6	42	theme	High	803:806	arg1	EE					808:809	High EE	803:809	High EE (>80%) of MPEP nanoliposomes	803:838	Rustles: High EE (>80%) of MPEP nanoliposomes was achieved.
31930869	10	43	theme	MPEP	1272:1275	arg1	nanoliposomes					1277:1289	The MPEP nanoliposomes	1268:1289	The MPEP nanoliposomes	1268:1289	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	4	44	theme	highest	600:606	arg1	EE					608:609	the highest EE	596:609	the highest EE in processed cheese	596:629	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	0	45	theme	Cheese	35:40	arg1	Production					0:9	Production	0:9	Production of Functional Processed Cheese	0:40	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	5	46	theme	physicochemical	709:723	arg1	properties					725:734	The physicochemical properties	705:734	The physicochemical properties	705:734	The physicochemical properties and phenolics content for processed cheese were analyzed.
31930869	4	47	theme	mandarin	478:485	arg1	powder					500:505	mandarin peel extract powder	478:505	mandarin peel extract powder (MPEP)	478:512	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	47	theme	mandarin	478:485	arg1	MPEP					508:511	MPEP	508:511	MPEP	508:511	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	3	48	theme	study	309:313	arg1	production					319:328	production	319:328	production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract	319:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	3	48	theme	study	309:313	arg1	objective					291:299	The objective	287:299	The objective of this study	287:313	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	8	49	theme	cheese	1060:1065	arg1	properties					1015:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties	989:1024	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	8	49	theme	cheese	1060:1065	arg1	parameters					1036:1045	color parameters	1030:1045	color parameters	1030:1045	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	10	50	theme	processed	1324:1332	arg1	cheese					1334:1339	processed cheese	1324:1339	processed cheese	1324:1339	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	7	51	theme	fat	967:969	arg1	cheese					981:986	half fat processed cheese	962:986	half fat processed cheese	962:986	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	4	52	theme	encapsulation	445:457	arg1	efficiency					459:468	The encapsulation efficiency	441:468	The encapsulation efficiency	441:468	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	2	53	theme	encapsulation	217:229	arg1	liposome					202:209	The liposome	198:209	The liposome	198:209	The liposome is an encapsulation method to incorporate the phenolics in functional food.
31930869	2	53	theme	encapsulation	217:229	arg1	method					231:236	an encapsulation method	214:236	an encapsulation method to incorporate the phenolics in functional food	214:284	The liposome is an encapsulation method to incorporate the phenolics in functional food.
31930869	1	54	theme	Mandarin	129:136	arg1	fruit					138:142	The Mandarin fruit	125:142	The Mandarin fruit	125:142	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	1	54	theme	Mandarin	129:136	arg1	source					159:164	a wonderful source	147:164	a wonderful source of essential dietary nutrients	147:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	4	55	from	ratios	677:682	arg1	nanoliposomes					660:672	MPEP nanoliposomes	655:672	MPEP nanoliposomes at ratios 25, 50 and 100% v/v	655:702	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	8	56	theme	processed	1050:1058	arg1	cheese					1060:1065	processed cheese	1050:1065	processed cheese supplemented with MPEP	1050:1088	Physical and organoleptic properties and color parameters of processed cheese supplemented with MPEP nanoliposomes inferior to the control.
31930869	2	57	theme	functional	270:279	arg1	food					281:284	functional food	270:284	functional food	270:284	The liposome is an encapsulation method to incorporate the phenolics in functional food.
31930869	10	58	theme	delivery	1375:1382	arg1	vesicle					1384:1390	a simple and effective delivery vesicle	1352:1390	a simple and effective delivery vesicle for phenolic compounds	1352:1413	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	4	59	theme	%	575:575	arg1	concentrations					535:548	five concentrations	530:548	five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v)	530:580	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	4	59	theme	%	575:575	arg1	w/v					577:579	0.2, 0.4, 0.6, 0.8 and 1% w/v	551:579	0.2, 0.4, 0.6, 0.8 and 1% w/v	551:579	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	5	60	theme	processed	762:770	arg1	cheese					772:777	processed cheese	762:777	processed cheese	762:777	The physicochemical properties and phenolics content for processed cheese were analyzed.
31930869	10	61	theme	simple	1354:1359	arg1	vesicle					1384:1390	a simple and effective delivery vesicle	1352:1390	a simple and effective delivery vesicle for phenolic compounds	1352:1413	The MPEP nanoliposomes were effectively retained within processed cheese, presented a simple and effective delivery vesicle for phenolic compounds.
31930869	4	62	dep	v/v	700:702	arg1	%					698:698	%	698:698	%	698:698	MATERIALS AND METHODS The encapsulation efficiency (EE) of mandarin peel extract powder (MPEP) was examined at five concentrations (0.2, 0.4, 0.6, 0.8 and 1% w/v) and inclusion the highest EE in processed cheese by replacing water with MPEP nanoliposomes at ratios 25, 50 and 100% v/v.
31930869	0	63	theme	Mandarin	77:84	arg1	Extract					91:97	Mandarin Peel Extract	77:97	Mandarin Peel Extract	77:97	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	3	64	theme	processed	344:352	arg1	cheese					354:359	functional processed cheese	333:359	functional processed cheese supplemented with nanoliposomes of mandarin peel extract	333:416	The objective of this study was production of functional processed cheese supplemented with nanoliposomes of mandarin peel extract.
31930869	9	65	theme	cold	1254:1257	arg1	storage					1259:1265	cold storage	1254:1265	cold storage	1254:1265	CONCLUSION The characterizations of processed cheese samples supplemented with MPEP nanoliposomes remained unaffected during cold storage.
31930869	0	66	theme	Functional	14:23	arg1	Cheese					35:40	Functional Processed Cheese	14:40	Functional Processed Cheese	14:40	Production of Functional Processed Cheese Supplemented with Nanoliposomes of Mandarin Peel Extract.
31930869	7	67	theme	processed	896:904	arg1	cheese					906:911	the resultant processed cheese	882:911	the resultant processed cheese	882:911	The chemical composition of the resultant processed cheese was in accordance with the Egyptian standard for half fat processed cheese.
31930869	1	68	theme	wonderful	149:157	arg1	fruit					138:142	The Mandarin fruit	125:142	The Mandarin fruit	125:142	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
31930869	1	68	theme	wonderful	149:157	arg1	source					159:164	a wonderful source	147:164	a wonderful source of essential dietary nutrients	147:195	BACKGROUND AND OBJECTIVE The Mandarin fruit is a wonderful source of essential dietary nutrients.
30872051	4	0	from	expressions	764:774	arg1	pathway					789:795	the NF-κB pathway	779:795	the NF-κB pathway	779:795	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	2	1	theme	MDA	365:367	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	2	2	theme	CYP2E1	461:466	arg1	factor					451:456	the inflammatory factor	434:456	the inflammatory factor of CYP2E1, TNF-α and IL-6	434:482	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	3	3	theme	TGF-β1	653:658	arg1	level					627:631	the level	623:631	the level of pivotal cytokine TGF-β1	623:658	ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-β1.
30872051	4	4	theme	Western	661:667	arg1	blotting					669:676	Western blotting	661:676	Western blotting	661:676	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	5	5	theme	Man	1006:1008	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	5	theme	Man	1006:1008	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	2	6	theme	CCl4-induced	557:568	arg1	injures					570:576	CCl4-induced injures	557:576	CCl4-induced injures	557:576	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	4	7	dep	results	678:684	arg1	revealed					686:693	revealed	686:693	results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway	678:795	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	2	8	theme	ALT	349:351	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	6	9	theme	natural	1140:1146	arg1	drugs					1148:1152	natural drugs	1140:1152	natural drugs	1140:1152	These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.
30872051	1	10	from	citrinipileatus	171:185	arg1	ERPS					150:153	ERPS	150:153	ERPS	150:153	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	1	10	from	citrinipileatus	171:185	arg1	polysaccharide					134:147	The enzymatic-extraction residue polysaccharide	101:147	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus	101:185	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	5	11	theme	β-type	883:888	arg1	ERPS					864:867	the ERPS	860:867	the ERPS	860:867	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	11	theme	β-type	883:888	arg1	pyranose					901:908	a typical β-type glycosidic pyranose	873:908	a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	873:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	4	12	theme	I-κBα	758:762	arg1	expressions					764:774	the I-κBα expressions	754:774	the I-κBα expressions in the NF-κB pathway	754:795	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	5	13	theme	GalUA	1049:1053	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	13	theme	GalUA	1049:1053	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	2	14	theme	IL-6	479:482	arg1	factor					451:456	the inflammatory factor	434:456	the inflammatory factor of CYP2E1, TNF-α and IL-6	434:482	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	5	15	theme	glycosidic	890:899	arg1	ERPS					864:867	the ERPS	860:867	the ERPS	860:867	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	15	theme	glycosidic	890:899	arg1	pyranose					901:908	a typical β-type glycosidic pyranose	873:908	a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	873:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	16	theme	molecular	934:942	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	17	theme	Glc	1016:1018	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	17	theme	Glc	1016:1018	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	6	18	theme	functional	1119:1128	arg1	foods					1130:1134	functional foods	1119:1134	functional foods	1119:1134	These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.
30872051	5	19	with	pyranose	901:908	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	19	with	pyranose	901:908	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	1	20	theme	enzymatic-extraction	105:124	arg1	ERPS					150:153	ERPS	150:153	ERPS	150:153	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	1	20	theme	enzymatic-extraction	105:124	arg1	polysaccharide					134:147	The enzymatic-extraction residue polysaccharide	101:147	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus	101:185	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	1	21	theme	antioxidant	209:219	arg1	effects					242:248	its antioxidant and hepatoprotective effects	205:248	its antioxidant and hepatoprotective effects	205:248	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	1	22	theme	residue	126:132	arg1	ERPS					150:153	ERPS	150:153	ERPS	150:153	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	1	22	theme	residue	126:132	arg1	polysaccharide					134:147	The enzymatic-extraction residue polysaccharide	101:147	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus	101:185	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	6	23	theme	liver	1179:1183	arg1	damage					1185:1190	the acute liver damage	1169:1190	the acute liver damage	1169:1190	These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.
30872051	2	24	theme	AST	344:346	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	5	25	theme	1.30 × 105 Da	954:966	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	25	theme	1.30 × 105 Da	954:966	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	1	26	theme	hepatoprotective	225:240	arg1	effects					242:248	its antioxidant and hepatoprotective effects	205:248	its antioxidant and hepatoprotective effects	205:248	The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated.
30872051	0	27	theme	hepatoprotective	16:31	arg1	activities					33:42	hepatoprotective activities	16:42	hepatoprotective activities	16:42	Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus.
30872051	5	28	theme	monosaccharide	976:989	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	4	29	theme	inflammatory	718:729	arg1	response					731:738	the inflammatory response	714:738	the inflammatory response	714:738	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	5	30	theme	structural	815:824	arg1	characteristics					826:840	the structural characteristics	811:840	the structural characteristics	811:840	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	0	31	theme	residue	47:53	arg1	polysaccharides					55:69	residue polysaccharides	47:69	residue polysaccharides	47:69	Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus.
30872051	2	32	theme	IL-10	501:505	arg1	levels					507:512	the IL-10 levels	497:512	the IL-10 levels	497:512	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	5	33	theme	GlcUA	1039:1043	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	33	theme	GlcUA	1039:1043	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	2	34	theme	GSH-Px	404:409	arg1	activities					390:399	the activities	386:399	the activities of GSH-Px, SOD and CAT	386:422	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	2	35	theme	Animal	274:279	arg1	experiments					281:291	Animal experiments	274:291	Animal experiments	274:291	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	2	36	theme	inflammatory	438:449	arg1	factor					451:456	the inflammatory factor	434:456	the inflammatory factor of CYP2E1, TNF-α and IL-6	434:482	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	0	37	theme	Pleurotus	74:82	arg1	citrinipileatus					84:98	Pleurotus citrinipileatus	74:98	Pleurotus citrinipileatus	74:98	Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus.
30872051	2	38	theme	TBIL	359:362	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	0	39	theme	polysaccharides	55:69	arg1	Antioxidant					0:10	Antioxidant	0:10	Antioxidant	0:10	Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus.
30872051	0	39	theme	polysaccharides	55:69	arg1	activities					33:42	hepatoprotective activities	16:42	hepatoprotective activities	16:42	Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus.
30872051	2	40	theme	potential	527:535	arg1	protections					537:547	the potential protections	523:547	the potential protections against CCl4-induced injures	523:576	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	3	41	theme	pivotal	636:642	arg1	TGF-β1					653:658	pivotal cytokine TGF-β1	636:658	pivotal cytokine TGF-β1	636:658	ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-β1.
30872051	5	42	theme	Gal	1021:1023	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	42	theme	Gal	1021:1023	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	3	43	theme	cytokine	644:651	arg1	TGF-β1					653:658	pivotal cytokine TGF-β1	636:658	pivotal cytokine TGF-β1	636:658	ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-β1.
30872051	6	44	theme	acute	1173:1177	arg1	damage					1185:1190	the acute liver damage	1169:1190	the acute liver damage	1169:1190	These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.
30872051	4	45	dep	ERPS	700:703	arg1	relieved					705:712	relieved	705:712	ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway	700:795	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	2	46	theme	ALP	354:356	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	3	47	theme	liver	596:600	arg1	fibrosis					602:609	liver fibrosis	596:609	liver fibrosis	596:609	ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-β1.
30872051	5	48	theme	weight-average	919:932	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	4	49	theme	NF-κB	783:787	arg1	pathway					789:795	the NF-κB pathway	779:795	the NF-κB pathway	779:795	Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway.
30872051	2	50	theme	LPO	373:375	arg1	levels					334:339	the levels	330:339	the levels of AST, ALT, ALP, TBIL, MDA and LPO	330:375	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	2	51	theme	TNF-α	469:473	arg1	factor					451:456	the inflammatory factor	434:456	the inflammatory factor of CYP2E1, TNF-α and IL-6	434:482	Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures.
30872051	5	52	theme	Rha	1011:1013	arg1	weight					944:949	the weight-average molecular weight	915:949	the weight-average molecular weight of 1.30 × 105 Da	915:966	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	52	theme	Rha	1011:1013	arg1	composition					991:1001	the monosaccharide composition	972:1001	the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	972:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	53	theme	typical	875:881	arg1	ERPS					864:867	the ERPS	860:867	the ERPS	860:867	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
30872051	5	53	theme	typical	875:881	arg1	pyranose					901:908	a typical β-type glycosidic pyranose	873:908	a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA	873:1053	Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA.
28623633	12	0	theme	initial	1373:1379	arg1	necrosis					1387:1394	the initial focal necrosis	1369:1394	the initial focal necrosis	1369:1394	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	13	1	theme	biodegradable	1594:1606	arg1	nails					1623:1627	novel biodegradable intramedullary nails	1588:1627	novel biodegradable intramedullary nails modified with long carbon and alginate fibers	1588:1673	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	12	2	theme	Histological	1335:1346	arg1	analysis					1348:1355	Histological analysis	1335:1355	Histological analysis	1335:1355	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	8	3	used	used	909:912	arg2	nails					867:871	The composite nails	853:871	The composite nails	853:871	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	8	3	used	used	909:912	arg2	wires					897:901	Kirschner wires	887:901	Kirschner wires	887:901	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	8	3	used	used	909:912	arg2	controls					877:884	controls	877:884	controls (Kirschner wires)	877:902	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	10	4	theme	Cell	1137:1140	arg1	tests					1142:1146	RESULTS Cell tests	1129:1146	RESULTS Cell tests	1129:1146	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	0	5	theme	In	79:80	arg1	biocompatibility					100:115	In vitro and in vivo biocompatibility	79:115	In vitro and in vivo biocompatibility	79:115	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	3	6	theme	in	481:482	arg1	assessments					506:516	in vitro and in vivo biocompatibility assessments	468:516	in vitro and in vivo biocompatibility assessments	468:516	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	1	7	theme	bone	240:243	arg1	fracture					245:252	the bone fracture	236:252	the bone fracture	236:252	BACKGROUND Commonly, intramedullary nails are made of nondegradable materials, and hence they need to be removed once the bone fracture is healed.
28623633	0	8	theme	in	92:93	arg1	biocompatibility					100:115	In vitro and in vivo biocompatibility	79:115	In vitro and in vivo biocompatibility	79:115	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	11	9	from	fixation	1284:1291	arg1	rabbits					1326:1332	rabbits	1326:1332	rabbits	1326:1332	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	8	10	theme	distal	930:935	arg1	osteotomy					945:953	distal femoral osteotomy	930:953	distal femoral osteotomy	930:953	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	0	11	dep	biocompatibility	100:115	arg1	nails					29:33	Biodegradable intramedullary nails	0:33	Biodegradable intramedullary nails reinforced with carbon and alginate fibers	0:76	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	9	12	theme	clinical	996:1003	arg1	observations					1005:1016	clinical observations	996:1016	clinical observations	996:1016	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	4	13	theme	biomaterials	575:586	arg1	biocompatibility					555:570	biocompatibility	555:570	biocompatibility of biomaterials	555:586	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	4	13	theme	biomaterials	575:586	arg1	conditions					543:552	the in vitro conditions	530:552	the in vitro conditions	530:552	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	13	14	theme	nails	1623:1627	arg1	biocompatibility					1540:1555	biocompatibility	1540:1555	biocompatibility	1540:1555	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	13	14	theme	nails	1623:1627	arg1	applicability					1571:1583	potential applicability	1561:1583	potential applicability	1561:1583	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	5	15	theme	proliferation	681:693	arg1	tests					695:699	cytotoxicity, viability and proliferation tests	653:699	cytotoxicity, viability and proliferation tests	653:699	After 3 and 7 days, cytotoxicity, viability and proliferation tests were performed, as well as cell morphology and adhesion observations.
28623633	6	16	theme	in	778:779	arg1	experiments					786:796	the in vivo experiments	774:796	the in vivo experiments	774:796	In the in vivo experiments, Californian rabbits (approx.
28623633	13	17	theme	alginate	1659:1666	arg1	fibers					1668:1673	alginate fibers	1659:1673	alginate fibers	1659:1673	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	9	18	theme	observations	1005:1016	arg1	basis					987:991	the basis	983:991	the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations	983:1126	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	11	19	theme	composite	1236:1244	arg1	nails					1246:1250	Biodegradable composite nails	1222:1250	Biodegradable composite nails	1222:1250	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	4	20	theme	human	614:618	arg1	osteoblasts					620:630	normal human osteoblasts	607:630	normal human osteoblasts	607:630	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	0	21	dep	in	92:93	arg1	vivo					95:98	vivo	95:98	vivo	95:98	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	10	22	theme	cell	1206:1209	arg1	viability					1211:1219	cell viability	1206:1219	cell viability	1206:1219	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	13	23	dep	in	1503:1504	arg1	vitro					1506:1510	vitro	1506:1510	vitro	1506:1510	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	11	24	theme	bony	1259:1262	arg1	union					1264:1268	bony union	1259:1268	bony union	1259:1268	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	4	25	dep	in	534:535	arg1	vitro					537:541	vitro	537:541	vitro	537:541	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	12	26	theme	trabeculae	1464:1473	arg1	formation					1475:1483	trabeculae formation	1464:1483	trabeculae formation	1464:1483	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	8	27	theme	osteotomy	945:953	arg1	fixation					918:925	fixation	918:925	fixation of distal femoral osteotomy	918:953	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	1	28	dep	BACKGROUND	118:127	arg1	need					212:215	need	212:215	need to be removed once the bone fracture is healed	212:262	BACKGROUND Commonly, intramedullary nails are made of nondegradable materials, and hence they need to be removed once the bone fracture is healed.
28623633	1	28	dep	BACKGROUND	118:127	arg1	made					164:167	made	164:167	are made of nondegradable materials	160:194	BACKGROUND Commonly, intramedullary nails are made of nondegradable materials, and hence they need to be removed once the bone fracture is healed.
28623633	9	29	theme	radiographs	1019:1029	arg1	basis					987:991	the basis	983:991	the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations	983:1126	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	1	30	theme	nondegradable	172:184	arg1	materials					186:194	nondegradable materials	172:194	nondegradable materials	172:194	BACKGROUND Commonly, intramedullary nails are made of nondegradable materials, and hence they need to be removed once the bone fracture is healed.
28623633	0	31	theme	intramedullary	14:27	arg1	nails					29:33	Biodegradable intramedullary nails	0:33	Biodegradable intramedullary nails reinforced with carbon and alginate fibers	0:76	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	11	32	theme	distal	1296:1301	arg1	osteotomy					1313:1321	distal diaphysis osteotomy	1296:1321	distal diaphysis osteotomy	1296:1321	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	8	33	theme	femoral	937:943	arg1	osteotomy					945:953	distal femoral osteotomy	930:953	distal femoral osteotomy	930:953	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	13	34	theme	in	1516:1517	arg1	tests					1524:1528	Both in vitro and in vivo tests	1498:1528	Both in vitro and in vivo tests	1498:1528	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	0	35	theme	Biodegradable	0:12	arg1	nails					29:33	Biodegradable intramedullary nails	0:33	Biodegradable intramedullary nails reinforced with carbon and alginate fibers	0:76	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	4	36	theme	in	534:535	arg1	biocompatibility					555:570	biocompatibility	555:570	biocompatibility of biomaterials	555:586	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	4	36	theme	in	534:535	arg1	conditions					543:552	the in vitro conditions	530:552	the in vitro conditions	530:552	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	11	37	theme	Biodegradable	1222:1234	arg1	nails					1246:1250	Biodegradable composite nails	1222:1250	Biodegradable composite nails	1222:1250	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	13	38	theme	bone	1697:1700	arg1	epiphysis					1702:1710	bone epiphysis	1697:1710	bone epiphysis	1697:1710	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	0	39	dep	In	79:80	arg1	vitro					82:86	vitro	82:86	vitro	82:86	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	6	40	dep	in	778:779	arg1	vivo					781:784	vivo	781:784	vivo	781:784	In the in vivo experiments, Californian rabbits (approx.
28623633	2	41	theme	alginate	364:371	arg1	fibers					373:378	alginate fibers	364:378	alginate fibers	364:378	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	10	42	contain	had	1178:1180	arg2	influence					1193:1201	a positive influence	1182:1201	a positive influence	1182:1201	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	10	42	contain	had	1178:1180	arg1	modifiers					1168:1176	both modifiers	1163:1176	both modifiers	1163:1176	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	13	43	theme	intramedullary	1608:1621	arg1	nails					1623:1627	novel biodegradable intramedullary nails	1588:1627	novel biodegradable intramedullary nails modified with long carbon and alginate fibers	1588:1673	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	9	44	theme	histological	1102:1113	arg1	observations					1115:1126	macroscopic and histological observations	1086:1126	macroscopic and histological observations	1086:1126	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	2	45	theme	novel	278:282	arg1	material					294:301	a novel composite material	276:301	a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation	276:431	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	3	46	theme	study	450:454	arg1	aim					438:440	The aim	434:440	The aim of this study	434:454	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	2	47	theme	composite	284:292	arg1	material					294:301	a novel composite material	276:301	a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation	276:431	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	3	48	dep	in	468:469	arg1	vitro					471:475	vitro	471:475	vitro	471:475	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	12	49	dep	proliferation	1446:1458	arg1	the					1431:1433	the	1431:1433	the	1431:1433	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	13	50	dep	in	1516:1517	arg1	vivo					1519:1522	vivo	1519:1522	vivo	1519:1522	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	2	51	theme	biodegradable	395:407	arg1	fixation					424:431	biodegradable intramedullary fixation	395:431	biodegradable intramedullary fixation	395:431	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	5	52	theme	adhesion	748:755	arg1	observations					757:768	adhesion observations	748:768	adhesion observations	748:768	After 3 and 7 days, cytotoxicity, viability and proliferation tests were performed, as well as cell morphology and adhesion observations.
28623633	6	53	dep	rabbits	811:817	arg1	experiments					786:796	the in vivo experiments	774:796	the in vivo experiments	774:796	In the in vivo experiments, Californian rabbits (approx.
28623633	13	54	theme	potential	1561:1569	arg1	applicability					1571:1583	potential applicability	1561:1583	potential applicability	1561:1583	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	8	55	theme	composite	857:865	arg1	nails					867:871	The composite nails	853:871	The composite nails	853:871	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	4	56	from	METHODS	519:525	arg1	biocompatibility					555:570	biocompatibility	555:570	biocompatibility of biomaterials	555:586	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	4	56	from	METHODS	519:525	arg1	conditions					543:552	the in vitro conditions	530:552	the in vitro conditions	530:552	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	13	57	theme	epiphysis	1702:1710	arg1	osteosynthesis					1679:1692	osteosynthesis	1679:1692	osteosynthesis of bone epiphysis	1679:1710	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	10	58	theme	RESULTS	1129:1135	arg1	tests					1142:1146	RESULTS Cell tests	1129:1146	RESULTS Cell tests	1129:1146	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	0	59	theme	alginate	62:69	arg1	fibers					71:76	alginate fibers	62:76	alginate fibers	62:76	Biodegradable intramedullary nails reinforced with carbon and alginate fibers: In vitro and in vivo biocompatibility.
28623633	12	60	theme	osteoblast	1435:1444	arg1	proliferation					1446:1458	osteoblast proliferation	1435:1458	osteoblast proliferation	1435:1458	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	11	61	theme	osteotomy	1313:1321	arg1	fixation					1284:1291	fixation	1284:1291	fixation of distal diaphysis osteotomy in rabbits	1284:1332	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	13	62	theme	in	1503:1504	arg1	tests					1524:1528	Both in vitro and in vivo tests	1498:1528	Both in vitro and in vivo tests	1498:1528	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	3	63	theme	in	468:469	arg1	assessments					506:516	in vitro and in vivo biocompatibility assessments	468:516	in vitro and in vivo biocompatibility assessments	468:516	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	5	64	theme	cytotoxicity	653:664	arg1	tests					695:699	cytotoxicity, viability and proliferation tests	653:699	cytotoxicity, viability and proliferation tests	653:699	After 3 and 7 days, cytotoxicity, viability and proliferation tests were performed, as well as cell morphology and adhesion observations.
28623633	3	65	dep	in	481:482	arg1	vivo					484:487	vivo	484:487	vivo	484:487	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	8	66	theme	Kirschner	887:895	arg1	controls					877:884	controls	877:884	controls (Kirschner wires)	877:902	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	8	66	theme	Kirschner	887:895	arg1	wires					897:901	Kirschner wires	887:901	Kirschner wires	887:901	The composite nails and controls (Kirschner wires) were used for fixation of distal femoral osteotomy.
28623633	2	67	theme	poly-L-lactide	317:330	arg1	matrix					332:337	poly-L-lactide matrix	317:337	poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation	317:431	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	13	68	theme	novel	1588:1592	arg1	nails					1623:1627	novel biodegradable intramedullary nails	1588:1627	novel biodegradable intramedullary nails modified with long carbon and alginate fibers	1588:1673	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	9	69	theme	observations	1115:1126	arg1	basis					987:991	the basis	983:991	the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations	983:1126	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	9	70	theme	macroscopic	1086:1096	arg1	observations					1115:1126	macroscopic and histological observations	1086:1126	macroscopic and histological observations	1086:1126	The evaluation was made on the basis of clinical observations, radiographs taken after 2, 4, 6 and 8 weeks post implantation, and macroscopic and histological observations.
28623633	5	71	theme	viability	667:675	arg1	tests					695:699	cytotoxicity, viability and proliferation tests	653:699	cytotoxicity, viability and proliferation tests	653:699	After 3 and 7 days, cytotoxicity, viability and proliferation tests were performed, as well as cell morphology and adhesion observations.
28623633	6	72	theme	Californian	799:809	arg1	rabbits					811:817	Californian rabbits	799:817	Californian rabbits (approx	799:825	In the in vivo experiments, Californian rabbits (approx.
28623633	11	73	theme	diaphysis	1303:1311	arg1	osteotomy					1313:1321	distal diaphysis osteotomy	1296:1321	distal diaphysis osteotomy	1296:1321	Biodegradable composite nails led to bony union when used for fixation of distal diaphysis osteotomy in rabbits.
28623633	2	74	theme	intramedullary	409:422	arg1	fixation					424:431	biodegradable intramedullary fixation	395:431	biodegradable intramedullary fixation	395:431	We propose a novel composite material consisting of poly-L-lactide matrix modified with carbon and alginate fibers to be used for biodegradable intramedullary fixation.
28623633	1	75	theme	intramedullary	139:152	arg1	nails					154:158	intramedullary nails	139:158	intramedullary nails	139:158	BACKGROUND Commonly, intramedullary nails are made of nondegradable materials, and hence they need to be removed once the bone fracture is healed.
28623633	4	76	theme	normal	607:612	arg1	osteoblasts					620:630	normal human osteoblasts	607:630	normal human osteoblasts	607:630	METHODS In the in vitro conditions, biocompatibility of biomaterials was compared using normal human osteoblasts.
28623633	5	77	theme	cell	728:731	arg1	morphology					733:742	cell morphology	728:742	cell morphology	728:742	After 3 and 7 days, cytotoxicity, viability and proliferation tests were performed, as well as cell morphology and adhesion observations.
28623633	12	78	theme	focal	1381:1385	arg1	necrosis					1387:1394	the initial focal necrosis	1369:1394	the initial focal necrosis	1369:1394	Histological analysis showed that the initial focal necrosis should be fully compensated for by the osteoblast proliferation and trabeculae formation.
28623633	13	79	dep	CONCLUSIONS	1486:1496	arg1	confirmed					1530:1538	confirmed	1530:1538	confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis	1530:1710	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
28623633	7	80	used	used	847:850	arg2	old					837:839	old	837:839	old	837:839	9 months old) were used.
28623633	3	81	theme	biocompatibility	489:504	arg1	assessments					506:516	in vitro and in vivo biocompatibility assessments	468:516	in vitro and in vivo biocompatibility assessments	468:516	The aim of this study was to make in vitro and in vivo biocompatibility assessments.
28623633	10	82	theme	positive	1184:1191	arg1	influence					1193:1201	a positive influence	1182:1201	a positive influence	1182:1201	RESULTS Cell tests indicated that both modifiers had a positive influence on cell viability.
28623633	13	83	theme	long	1643:1646	arg1	carbon					1648:1653	long carbon	1643:1653	long carbon	1643:1653	CONCLUSIONS Both in vitro and in vivo tests confirmed biocompatibility and potential applicability of novel biodegradable intramedullary nails modified with long carbon and alginate fibers for osteosynthesis of bone epiphysis.
31826404	4	0	theme	composite	864:872	arg1	membranes					874:882	composite membranes	864:882	composite membranes	864:882	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	3	1	theme	remarkable	560:569	arg1	properties					585:594	remarkable antibacterial properties	560:594	remarkable antibacterial properties against both bacteria	560:616	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	1	2	theme	BC	283:284	arg1	matrix					287:292	bacterial cellulose (BC) matrix	262:292	bacterial cellulose (BC) matrix respectively	262:305	To synthesize biomaterials for antibacterial applications, chitosan (CS) and chitooligosaccharide (COS) were successfully incorporated into bacterial cellulose (BC) matrix respectively.
31826404	3	3	theme	%	724:724	arg1	ratio					638:642	the inhibition ratio	623:642	the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS	623:775	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	3	4	theme	antibacterial	571:583	arg1	properties					585:594	remarkable antibacterial properties	560:594	remarkable antibacterial properties against both bacteria	560:616	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	2	5	theme	Gram-positive	327:339	arg1	bacteria					341:348	The representative Gram-positive bacteria	308:348	The representative Gram-positive bacteria (Staphylococcus aureus)	308:372	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	4	6	dep	showed	840:845	arg1	possessed					884:892	possessed	884:892	showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC	840:953	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	4	7	theme	pure	947:950	arg1	BC					952:953	pure BC	947:953	pure BC	947:953	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	2	8	theme	representative	312:325	arg1	bacteria					341:348	The representative Gram-positive bacteria	308:348	The representative Gram-positive bacteria (Staphylococcus aureus)	308:372	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	3	9	theme	%	754:754	arg1	ratio					638:642	the inhibition ratio	623:642	the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS	623:775	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	3	10	dep	%	659:659	arg1	aureus					665:670	S. aureus	662:670	S. aureus	662:670	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	5	11	with	contrast	969:976	arg1	BC-CS					983:987	BC-CS	983:987	BC-CS	983:987	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	4	12	dep	BC-CS	847:851	arg1	membranes					874:882	composite membranes	864:882	composite membranes	864:882	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	5	13	theme	mechanical	1014:1023	arg1	properties					1025:1034	better mechanical properties	1007:1034	better mechanical properties	1007:1034	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	6	14	theme	medical	1266:1272	arg1	fields					1274:1279	food and medical fields	1257:1279	food and medical fields	1257:1279	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	6	15	theme	composite	1170:1178	arg1	membrane					1180:1187	BC-COS composite membrane	1163:1187	BC-COS composite membrane	1163:1187	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	5	16	theme	%	1112:1112	arg1	capacity					1088:1095	stronger ABTS radical scavenging capacity	1055:1095	stronger ABTS radical scavenging capacity of 92.62 ± 2.85%	1055:1112	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	2	17	theme	membranes	489:497	arg1	effects					464:470	the antibacterial effects	446:470	the antibacterial effects of the composite membranes	446:497	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	3	18	dep	%	724:724	arg1	aureus					730:735	S. aureus	727:735	S. aureus	727:735	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	5	19	theme	ABTS	1064:1067	arg1	capacity					1088:1095	stronger ABTS radical scavenging capacity	1055:1095	stronger ABTS radical scavenging capacity of 92.62 ± 2.85%	1055:1112	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	6	20	theme	BC-COS	1163:1168	arg1	membrane					1180:1187	BC-COS composite membrane	1163:1187	BC-COS composite membrane	1163:1187	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	0	21	theme	bacterial	34:42	arg1	membranes					64:72	antibacterial bacterial cellulose composite membranes	20:72	antibacterial bacterial cellulose composite membranes	20:72	Characterization of antibacterial bacterial cellulose composite membranes modified with chitosan or chitooligosaccharide.
31826404	3	22	theme	inhibition	627:636	arg1	ratio					638:642	the inhibition ratio	623:642	the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS	623:775	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	6	23	theme	food	1257:1260	arg1	fields					1274:1279	food and medical fields	1257:1279	food and medical fields	1257:1279	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	0	24	theme	antibacterial	20:32	arg1	membranes					64:72	antibacterial bacterial cellulose composite membranes	20:72	antibacterial bacterial cellulose composite membranes	20:72	Characterization of antibacterial bacterial cellulose composite membranes modified with chitosan or chitooligosaccharide.
31826404	5	25	theme	radical	1069:1075	arg1	capacity					1088:1095	stronger ABTS radical scavenging capacity	1055:1095	stronger ABTS radical scavenging capacity of 92.62 ± 2.85%	1055:1112	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	0	26	theme	composite	54:62	arg1	membranes					64:72	antibacterial bacterial cellulose composite membranes	20:72	antibacterial bacterial cellulose composite membranes	20:72	Characterization of antibacterial bacterial cellulose composite membranes modified with chitosan or chitooligosaccharide.
31826404	2	27	dep	bacteria	341:348	arg1	aureus					366:371	Staphylococcus aureus	351:371	Staphylococcus aureus	351:371	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	5	28	theme	scavenging	1077:1086	arg1	capacity					1088:1095	stronger ABTS radical scavenging capacity	1055:1095	stronger ABTS radical scavenging capacity of 92.62 ± 2.85%	1055:1112	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	5	29	theme	better	1007:1012	arg1	properties					1025:1034	better mechanical properties	1007:1034	better mechanical properties	1007:1034	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	2	30	theme	antibacterial	450:462	arg1	effects					464:470	the antibacterial effects	446:470	the antibacterial effects of the composite membranes	446:497	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	0	31	theme	cellulose	44:52	arg1	membranes					64:72	antibacterial bacterial cellulose composite membranes	20:72	antibacterial bacterial cellulose composite membranes	20:72	Characterization of antibacterial bacterial cellulose composite membranes modified with chitosan or chitooligosaccharide.
31826404	2	32	theme	composite	479:487	arg1	membranes					489:497	the composite membranes	475:497	the composite membranes	475:497	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	3	33	dep	%	689:689	arg1	coli					695:698	E. coli	692:698	E. coli	692:698	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	4	34	theme	porosity	900:907	arg1	structure					920:928	lower porosity and denser structure	894:928	lower porosity and denser structure	894:928	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	3	35	theme	%	659:659	arg1	ratio					638:642	the inhibition ratio	623:642	the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS	623:775	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	0	36	theme	membranes	64:72	arg1	Characterization					0:15	Characterization	0:15	Characterization of antibacterial bacterial cellulose composite membranes	0:72	Characterization of antibacterial bacterial cellulose composite membranes modified with chitosan or chitooligosaccharide.
31826404	6	37	theme	antibacterial	1227:1239	arg1	applications					1241:1252	the antibacterial applications	1223:1252	the antibacterial applications in food and medical fields	1223:1279	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	1	38	theme	antibacterial	153:165	arg1	applications					167:178	antibacterial applications	153:178	antibacterial applications	153:178	To synthesize biomaterials for antibacterial applications, chitosan (CS) and chitooligosaccharide (COS) were successfully incorporated into bacterial cellulose (BC) matrix respectively.
31826404	6	39	from	applications	1241:1252	arg1	fields					1274:1279	food and medical fields	1257:1279	food and medical fields	1257:1279	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	3	40	dep	%	754:754	arg1	coli					760:763	E. coli	757:763	E. coli	757:763	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	4	41	theme	denser	913:918	arg1	structure					920:928	lower porosity and denser structure	894:928	lower porosity and denser structure	894:928	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	4	42	theme	electron	814:821	arg1	microscopy					823:832	scanning electron microscopy	805:832	scanning electron microscopy (SEM)	805:838	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	4	42	theme	electron	814:821	arg1	SEM					835:837	SEM	835:837	SEM	835:837	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	2	43	dep	bacteria	392:399	arg1	coli					414:417	Escherichia coli	402:417	Escherichia coli	402:417	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	6	44	theme	membrane	1180:1187	arg1	development					1148:1158	the development	1144:1158	the development of BC-COS composite membrane	1144:1187	These results suggested that the development of BC-COS composite membrane would open up the possibility for the antibacterial applications in food and medical fields.
31826404	5	45	theme	stronger	1055:1062	arg1	capacity					1088:1095	stronger ABTS radical scavenging capacity	1055:1095	stronger ABTS radical scavenging capacity of 92.62 ± 2.85%	1055:1112	Moreover, in contrast with BC-CS, BC-COS performed better mechanical properties and also displayed stronger ABTS radical scavenging capacity of 92.62 ± 2.85%.
31826404	3	46	theme	%	689:689	arg1	ratio					638:642	the inhibition ratio	623:642	the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS	623:775	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	3	47	theme	BC-COS	533:538	arg1	membranes					540:548	BC-COS membranes	533:548	BC-COS membranes	533:548	The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.99 ± 0.01% (S. aureus) and 99.99 ± 0.01% (E. coli) for BC-CS, 99.64 ± 0.18% (S. aureus) and 90.56 ± 0.06% (E. coli) for BC-COS.
31826404	1	48	theme	bacterial	262:270	arg1	matrix					287:292	bacterial cellulose (BC) matrix	262:292	bacterial cellulose (BC) matrix respectively	262:305	To synthesize biomaterials for antibacterial applications, chitosan (CS) and chitooligosaccharide (COS) were successfully incorporated into bacterial cellulose (BC) matrix respectively.
31826404	2	49	theme	Gram-negative	378:390	arg1	bacteria					392:399	Gram-negative bacteria	378:399	Gram-negative bacteria (Escherichia coli)	378:418	The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes.
31826404	4	50	theme	scanning	805:812	arg1	microscopy					823:832	scanning electron microscopy	805:832	scanning electron microscopy (SEM)	805:838	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	4	50	theme	scanning	805:812	arg1	SEM					835:837	SEM	835:837	SEM	835:837	The morphology obtained by scanning electron microscopy (SEM) showed BC-CS and BC-COS composite membranes possessed lower porosity and denser structure in comparison to pure BC.
31826404	1	51	theme	cellulose	272:280	arg1	matrix					287:292	bacterial cellulose (BC) matrix	262:292	bacterial cellulose (BC) matrix respectively	262:305	To synthesize biomaterials for antibacterial applications, chitosan (CS) and chitooligosaccharide (COS) were successfully incorporated into bacterial cellulose (BC) matrix respectively.
33463242	1	0	theme	increasing	205:214	arg1	attention					216:224	increasing attention	205:224	increasing attention	205:224	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	4	1	from	similar	949:955	arg1	composition					960:970	composition	960:970	composition	960:970	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	4	1	from	similar	949:955	arg1	structure					976:984	structure	976:984	structure	976:984	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	6	2	theme	mouse	1104:1108	arg1	cells					1139:1143	mouse bone marrow mesenchymal stem cells	1104:1143	mouse bone marrow mesenchymal stem cells	1104:1143	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	0	3	theme	Bone	124:127	arg1	Regeneration					129:140	Bone Regeneration	124:140	Bone Regeneration	124:140	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	4	4	from	composition	960:970	arg1	similar					949:955	similar	949:955	similar	949:955	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	7	5	theme	CS/HPC/nHA	1350:1359	arg1	scaffold					1371:1378	the CS/HPC/nHA composite scaffold	1346:1378	the CS/HPC/nHA composite scaffold	1346:1378	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	3	6	theme	X-ray	724:728	arg1	spectroscopy					744:755	X-ray photoelectron spectroscopy	724:755	X-ray photoelectron spectroscopy	724:755	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	7	7	theme	In	1301:1302	arg1	experiments					1309:1319	In vivo experiments	1301:1319	In vivo experiments	1301:1319	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	8	8	theme	application	1528:1538	arg1	prospect					1540:1547	great application prospect	1522:1547	great application prospect	1522:1547	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	8	9	theme	great	1522:1526	arg1	prospect					1540:1547	great application prospect	1522:1547	great application prospect	1522:1547	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	6	10	theme	osteogenesis	1277:1288	arg1	direction					1290:1298	the osteogenesis direction	1273:1298	the osteogenesis direction	1273:1298	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	3	11	theme	scanning	627:634	arg1	microscopy					645:654	scanning electron microscopy	627:654	scanning electron microscopy	627:654	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	12	theme	mechanical	796:805	arg1	properties					807:816	mechanical properties	796:816	mechanical properties	796:816	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	5	13	theme	certain	1062:1068	arg1	strength					1081:1088	a certain mechanical strength	1060:1088	a certain mechanical strength	1060:1088	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	2	14	theme	three-dimensional	336:352	arg1	CS					364:365	CS	364:365	CS	364:365	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	2	14	theme	three-dimensional	336:352	arg1	chitosan					354:361	a three-dimensional chitosan	334:361	a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite	334:415	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	7	15	contain	has	1380:1382	arg2	advantage					1398:1406	a significant advantage	1384:1406	a significant advantage	1384:1406	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	7	15	contain	has	1380:1382	arg1	scaffold					1371:1378	the CS/HPC/nHA composite scaffold	1346:1378	the CS/HPC/nHA composite scaffold	1346:1378	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	3	16	dep	Fourier	692:698	arg1	transform					700:708	transform	700:708	transform infrared	700:717	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	5	17	theme	mechanical	1070:1079	arg1	strength					1081:1088	a certain mechanical strength	1060:1088	a certain mechanical strength	1060:1088	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	5	18	theme	composite	1019:1027	arg1	scaffold					1029:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	7	19	theme	bone	1443:1446	arg1	area					1455:1458	the bone defect area	1439:1458	the bone defect area	1439:1458	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	3	20	theme	CS/HPC/nHA	594:603	arg1	structure					565:573	structure	565:573	structure	565:573	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	20	theme	CS/HPC/nHA	594:603	arg1	composition					579:589	composition	579:589	composition	579:589	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	6	21	theme	scaffold	1167:1174	arg1	surface					1152:1158	the surface	1148:1158	the surface of the scaffold	1148:1174	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	0	22	theme	High-Porosity	18:30	arg1	Carbon/Hydroxyapatite					58:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	8	23	theme	CS/HPC/nHA	1496:1505	arg1	scaffolds					1507:1515	the CS/HPC/nHA scaffolds	1492:1515	the CS/HPC/nHA scaffolds	1492:1515	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	3	24	theme	electron	636:643	arg1	microscopy					645:654	scanning electron microscopy	627:654	scanning electron microscopy	627:654	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	1	25	theme	due	244:246	arg1	bioengineering					229:242	bioengineering	229:242	bioengineering due to excellent mechanical properties and a high surface-to-volume ratio	229:316	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	0	26	theme	Three-Dimensional	0:16	arg1	Carbon/Hydroxyapatite					58:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	3	27	theme	scaffold	825:832	arg1	porosity					766:773	the porosity	762:773	the porosity	762:773	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	27	theme	scaffold	825:832	arg1	ratio					785:789	swelling ratio	776:789	swelling ratio	776:789	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	27	theme	scaffold	825:832	arg1	properties					807:816	mechanical properties	796:816	mechanical properties	796:816	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	2	28	from	hydroxyapatite	444:457	arg1	surface					476:482	the HPC surface	468:482	the HPC surface	468:482	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	2	28	from	hydroxyapatite	444:457	arg1	deposition					492:501	situ deposition	487:501	situ deposition	487:501	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	1	29	theme	Three-dimensional	143:159	arg1	HPC					186:188	HPC	186:188	HPC	186:188	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	1	29	theme	Three-dimensional	143:159	arg1	carbon					178:183	Three-dimensional honeycomb porous carbon	143:183	Three-dimensional honeycomb porous carbon (HPC)	143:189	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	8	30	theme	tissue	1557:1562	arg1	engineering					1564:1574	bone tissue engineering	1552:1574	bone tissue engineering	1552:1574	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	0	31	theme	Porous	51:56	arg1	Carbon/Hydroxyapatite					58:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	8	32	theme	bone	1552:1555	arg1	engineering					1564:1574	bone tissue engineering	1552:1574	bone tissue engineering	1552:1574	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	4	33	theme	organic-inorganic	909:925	arg1	components					927:936	organic-inorganic components	909:936	organic-inorganic components	909:936	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	1	34	theme	honeycomb	161:169	arg1	HPC					186:188	HPC	186:188	HPC	186:188	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	1	34	theme	honeycomb	161:169	arg1	carbon					178:183	Three-dimensional honeycomb porous carbon	143:183	Three-dimensional honeycomb porous carbon (HPC)	143:189	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	1	35	theme	excellent	251:259	arg1	properties					272:281	excellent mechanical properties	251:281	excellent mechanical properties	251:281	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	2	36	theme	situ	487:490	arg1	deposition					492:501	situ deposition	487:501	situ deposition	487:501	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	5	37	theme	high	1042:1045	arg1	porosity					1047:1054	high porosity	1042:1054	high porosity	1042:1054	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	2	38	from	vacuum	540:545	arg1	surface					476:482	the HPC surface	468:482	the HPC surface	468:482	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	2	38	from	vacuum	540:545	arg1	deposition					492:501	situ deposition	487:501	situ deposition	487:501	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	1	39	theme	porous	171:176	arg1	HPC					186:188	HPC	186:188	HPC	186:188	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	1	39	theme	porous	171:176	arg1	carbon					178:183	Three-dimensional honeycomb porous carbon	143:183	Three-dimensional honeycomb porous carbon (HPC)	143:189	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	2	40	theme	chitosan	517:524	arg1	solution					526:533	chitosan solution	517:533	chitosan solution	517:533	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	1	41	theme	mechanical	261:270	arg1	properties					272:281	excellent mechanical properties	251:281	excellent mechanical properties	251:281	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	8	42	contain	have	1517:1520	arg2	prospect					1540:1547	great application prospect	1522:1547	great application prospect	1522:1547	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	8	42	contain	have	1517:1520	arg1	scaffolds					1507:1515	the CS/HPC/nHA scaffolds	1492:1515	the CS/HPC/nHA scaffolds	1492:1515	All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
33463242	5	43	theme	synthesized	1007:1017	arg1	scaffold					1029:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	3	44	theme	photoelectron	730:742	arg1	spectroscopy					744:755	X-ray photoelectron spectroscopy	724:755	X-ray photoelectron spectroscopy	724:755	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	45	theme	electron	670:677	arg1	miscroscopy					679:689	transmission electron miscroscopy	657:689	transmission electron miscroscopy	657:689	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	5	46	contain	has	1038:1040	arg2	strength					1081:1088	a certain mechanical strength	1060:1088	a certain mechanical strength	1060:1088	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	5	46	contain	has	1038:1040	arg2	porosity					1047:1054	high porosity	1042:1054	high porosity	1042:1054	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	5	46	contain	has	1038:1040	arg1	scaffold					1029:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold	1003:1036	The synthesized composite scaffold has high porosity and a certain mechanical strength.
33463242	2	47	theme	HPC	472:474	arg1	surface					476:482	the HPC surface	468:482	the HPC surface	468:482	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	6	48	theme	stem	1134:1137	arg1	cells					1139:1143	mouse bone marrow mesenchymal stem cells	1104:1143	mouse bone marrow mesenchymal stem cells	1104:1143	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	6	49	theme	marrow	1115:1120	arg1	cells					1139:1143	mouse bone marrow mesenchymal stem cells	1104:1143	mouse bone marrow mesenchymal stem cells	1104:1143	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	0	50	theme	Enhanced	94:101	arg1	Osteoinductivity					103:118	Enhanced Osteoinductivity	94:118	Enhanced Osteoinductivity for Bone Regeneration	94:140	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	4	51	theme	bone	989:992	arg1	tissues					994:1000	bone tissues	989:1000	bone tissues	989:1000	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	3	52	dep	transform	700:708	arg1	infrared					710:717	infrared	710:717	transform infrared	700:717	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	7	53	theme	significant	1386:1396	arg1	advantage					1398:1406	a significant advantage	1384:1406	a significant advantage	1384:1406	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	4	54	from	structure	976:984	arg1	similar					949:955	similar	949:955	similar	949:955	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	3	55	theme	transmission	657:668	arg1	miscroscopy					679:689	transmission electron miscroscopy	657:689	transmission electron miscroscopy	657:689	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	3	56	dep	structure	565:573	arg1	The					561:563	The	561:563	The	561:563	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	7	57	dep	In	1301:1302	arg1	vivo					1304:1307	vivo	1304:1307	vivo	1304:1307	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	6	58	theme	mesenchymal	1122:1132	arg1	cells					1139:1143	mouse bone marrow mesenchymal stem cells	1104:1143	mouse bone marrow mesenchymal stem cells	1104:1143	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
33463242	2	59	theme	carbon/hydroxyapatite	385:405	arg1	composite					407:415	porous carbon/hydroxyapatite composite	378:415	porous carbon/hydroxyapatite composite	378:415	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	4	60	contain	possess	878:884	arg2	components					927:936	organic-inorganic components	909:936	organic-inorganic components	909:936	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	4	60	contain	possess	878:884	arg1	scaffolds					868:876	The as-prepared scaffolds	852:876	The as-prepared scaffolds	852:876	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	4	60	contain	possess	878:884	arg2	pores					899:903	hierarchical pores	886:903	hierarchical pores	886:903	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	4	61	theme	as-prepared	856:866	arg1	scaffolds					868:876	The as-prepared scaffolds	852:876	The as-prepared scaffolds	852:876	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	2	62	theme	porous	378:383	arg1	composite					407:415	porous carbon/hydroxyapatite composite	378:415	porous carbon/hydroxyapatite composite	378:415	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	1	63	theme	high	289:292	arg1	ratio					312:316	a high surface-to-volume ratio	287:316	a high surface-to-volume ratio	287:316	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	4	64	theme	hierarchical	886:897	arg1	pores					899:903	hierarchical pores	886:903	hierarchical pores	886:903	The as-prepared scaffolds possess hierarchical pores and organic-inorganic components, which are similar in composition and structure to bone tissues.
33463242	7	65	theme	bone	1421:1424	arg1	formation					1426:1434	bone formation	1421:1434	bone formation	1421:1434	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	7	66	theme	composite	1361:1369	arg1	scaffold					1371:1378	the CS/HPC/nHA composite scaffold	1346:1378	the CS/HPC/nHA composite scaffold	1346:1378	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	7	67	theme	defect	1448:1453	arg1	area					1455:1458	the bone defect area	1439:1458	the bone defect area	1439:1458	In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area.
33463242	1	68	theme	surface-to-volume	294:310	arg1	ratio					312:316	a high surface-to-volume ratio	287:316	a high surface-to-volume ratio	287:316	Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio.
33463242	2	69	theme	freeze-dried	547:558	arg1	vacuum					540:545	vacuum freeze-dried	540:558	vacuum freeze-dried	540:558	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	3	70	theme	swelling	776:783	arg1	ratio					785:789	swelling ratio	776:789	swelling ratio	776:789	The structure and composition of CS/HPC/nHA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photoelectron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested.
33463242	0	71	theme	Chitosan/Honeycomb	32:49	arg1	Carbon/Hydroxyapatite					58:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite	0:78	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	0	72	with	Scaffold	80:87	arg1	Osteoinductivity					103:118	Enhanced Osteoinductivity	94:118	Enhanced Osteoinductivity for Bone Regeneration	94:140	Three-Dimensional High-Porosity Chitosan/Honeycomb Porous Carbon/Hydroxyapatite Scaffold with Enhanced Osteoinductivity for Bone Regeneration.
33463242	2	73	theme	nano-sized	433:442	arg1	nHA					460:462	nHA	460:462	nHA	460:462	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	2	73	theme	nano-sized	433:442	arg1	hydroxyapatite					444:457	nano-sized hydroxyapatite	433:457	nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution,	433:534	In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried.
33463242	6	74	theme	bone	1110:1113	arg1	cells					1139:1143	mouse bone marrow mesenchymal stem cells	1104:1143	mouse bone marrow mesenchymal stem cells	1104:1143	By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction.
29587830	11	0	theme	algal	2150:2154	arg1	polysaccharides					2156:2170	algal polysaccharides	2150:2170	algal polysaccharides	2150:2170	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	2	1	theme	complex	360:366	arg1	polysaccharides					368:382	complex polysaccharides	360:382	complex polysaccharides from land plants	360:399	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	9	2	theme	primary	1689:1695	arg1	degraders					1697:1705	aerobic primary degraders	1681:1705	aerobic primary degraders of algal polysaccharides	1681:1730	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	7	3	theme	algal	1448:1452	arg1	diet					1454:1457	a specific algal diet	1437:1457	a specific algal diet	1437:1457	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	11	4	from	niche	2074:2078	arg1	holobiont					2095:2103	the abalone holobiont	2083:2103	the abalone holobiont	2083:2103	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	9	5	theme	algal	1710:1714	arg1	polysaccharides					1716:1730	algal polysaccharides	1710:1730	algal polysaccharides	1710:1730	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	1	6	contain	have	167:170	arg1	Holobionts					156:165	BACKGROUND Holobionts	145:165	BACKGROUND Holobionts	145:165	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	1	6	contain	have	167:170	arg2	microbiota					184:193	a digestive microbiota	172:193	a digestive microbiota	172:193	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	2	7	theme	terrestrial	291:301	arg1	herbivores					303:312	terrestrial herbivores	291:312	terrestrial herbivores	291:312	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	10	8	theme	persistent	1790:1799	arg1	microbiota					1806:1815	a persistent core microbiota	1788:1815	a persistent core microbiota in the digestive gland of the abalone since its juvenile state	1788:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	1	9	theme	digestive	174:182	arg1	microbiota					184:193	a digestive microbiota	172:193	a digestive microbiota	172:193	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	9	10	theme	polysaccharides	1716:1730	arg1	degraders					1697:1705	aerobic primary degraders	1681:1705	aerobic primary degraders of algal polysaccharides	1681:1730	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	11	11	theme	algal	2289:2293	arg1	microbiota					2295:2304	the aerobic algal microbiota	2277:2304	the aerobic algal microbiota	2277:2304	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	2	12	theme	digestive	319:327	arg1	microbiota					329:338	the digestive microbiota	315:338	the digestive microbiota	315:338	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	5	13	dep	composition	1000:1010	arg1	the					996:998	the	996:998	the	996:998	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	14	theme	marine	1072:1077	arg1	herbivore					1079:1087	a generalist marine herbivore	1059:1087	a generalist marine herbivore	1059:1087	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	14	theme	marine	1072:1077	arg1	abalone					1094:1100	the abalone	1090:1100	the abalone	1090:1100	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	3	15	theme	red	526:528	arg1	macroalgae					548:557	red, green, and brown macroalgae	526:557	red, green, and brown macroalgae	526:557	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	7	16	theme	contextual	1395:1404	arg1	parameters					1406:1415	contextual parameters	1395:1415	contextual parameters	1395:1415	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	11	17	dep	products	2175:2182	arg1	acquired					2225:2232	acquired	2225:2232	(ii) may have acquired these functions through gene transfer from the aerobic algal microbiota	2211:2304	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	11	17	dep	products	2175:2182	arg1	assimilable					2184:2194	assimilable	2184:2194	assimilable	2184:2194	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	0	18	theme	marine	127:132	arg1	herbivore					134:142	a generalist marine herbivore	114:142	a generalist marine herbivore	114:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	0	18	theme	marine	127:132	arg1	tuberculata					101:111	the European abalone Haliotis tuberculata	71:111	the European abalone Haliotis tuberculata	71:111	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	3	19	theme	specific	603:610	arg1	composition					612:622	a specific composition	601:622	a specific composition	601:622	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	8	20	theme	core	1466:1469	arg1	genera					1471:1476	Three core genera	1460:1476	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio	1460:1518	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	5	21	theme	microbiota	1045:1054	arg1	composition					1000:1010	composition	1000:1010	composition	1000:1010	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	21	theme	microbiota	1045:1054	arg1	specificity					1016:1026	specificity	1016:1026	specificity	1016:1026	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	11	22	theme	digestive	2033:2041	arg1	gland					2043:2047	the digestive gland	2029:2047	the digestive gland	2029:2047	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	4	23	theme	epiphytic	740:748	arg1	microbiota					750:759	a specific epiphytic microbiota	729:759	a specific epiphytic microbiota	729:759	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	11	24	theme	particular	2063:2072	arg1	niche					2074:2078	a particular niche	2061:2078	a particular niche	2061:2078	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	5	25	from	effect	884:889	arg1	composition					1000:1010	composition	1000:1010	composition	1000:1010	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	25	from	effect	884:889	arg1	specificity					1016:1026	specificity	1016:1026	specificity	1016:1026	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	6	26	theme	digestive	1183:1191	arg1	gland					1193:1197	the abalone digestive gland	1171:1197	the abalone digestive gland	1171:1197	The microbiota from the abalone digestive gland was sampled every 2 months and explored using metabarcoding.
29587830	11	27	theme	microbial	1978:1986	arg1	taxa					1988:1991	different microbial taxa	1968:1991	different microbial taxa	1968:1991	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	7	28	attach	linked	1362:1367	arg2	patterns					1316:1323	patterns	1316:1323	patterns of the microbiota	1316:1341	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	7	28	attach	linked	1362:1367	arg1	variations					1381:1390	seasonal variations	1372:1390	seasonal variations of contextual parameters	1372:1415	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	7	28	attach	linked	1362:1367	arg1	diet					1454:1457	a specific algal diet	1437:1457	a specific algal diet	1437:1457	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	0	29	theme	microbiota	57:66	arg1	patterns					31:38	Seasonal and algal diet-driven patterns	0:38	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.	0:143	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	10	30	theme	juvenile	1865:1872	arg1	state					1874:1878	its juvenile state	1861:1878	its juvenile state	1861:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	5	31	theme	monospecific	899:910	arg1	diets					912:916	four monospecific diets	894:916	four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata)	894:991	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	32	theme	coastal	1125:1131	arg1	area					1133:1136	a temperate coastal area	1113:1136	a temperate coastal area over a year	1113:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	6	33	from	gland	1193:1197	arg1	microbiota					1155:1164	The microbiota	1151:1164	The microbiota from the abalone digestive gland	1151:1197	The microbiota from the abalone digestive gland was sampled every 2 months and explored using metabarcoding.
29587830	0	34	theme	abalone	84:90	arg1	herbivore					134:142	a generalist marine herbivore	114:142	a generalist marine herbivore	114:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	0	34	theme	abalone	84:90	arg1	tuberculata					101:111	the European abalone Haliotis tuberculata	71:111	the European abalone Haliotis tuberculata	71:111	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	10	35	theme	microbiota	1806:1815	arg1	establishment					1771:1783	the establishment	1767:1783	the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state	1767:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	10	35	theme	microbiota	1806:1815	arg1	presence					1888:1895	the presence	1884:1895	the presence of a less abundant and diet-specific core community	1884:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	11	36	theme	gene	2258:2261	arg1	transfer					2263:2270	gene transfer	2258:2270	gene transfer from the aerobic algal microbiota	2258:2304	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	4	37	theme	marine	794:799	arg1	herbivores					801:810	marine herbivores	794:810	marine herbivores	794:810	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	2	38	theme	marine	410:415	arg1	herbivores					417:426	marine herbivores	410:426	marine herbivores	410:426	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	0	39	theme	tuberculata	101:111	arg1	microbiota					57:66	the digestive microbiota	43:66	the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore	43:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	5	40	theme	farmed	1103:1108	arg1	herbivore					1079:1087	a generalist marine herbivore	1059:1087	a generalist marine herbivore	1059:1087	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	40	theme	farmed	1103:1108	arg1	abalone					1094:1100	the abalone	1090:1100	the abalone	1090:1100	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	41	dep	diets	912:916	arg1	palmata					928:934	Palmaria palmata	919:934	Palmaria palmata	919:934	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	41	dep	diets	912:916	arg1	lactuca					942:948	Ulva lactuca	937:948	Ulva lactuca	937:948	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	41	dep	diets	912:916	arg1	latissima					962:970	Saccharina latissima	951:970	Saccharina latissima	951:970	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	41	dep	diets	912:916	arg1	digitata					983:990	Laminaria digitata	973:990	Laminaria digitata	973:990	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	0	42	theme	Seasonal	0:7	arg1	patterns					31:38	Seasonal and algal diet-driven patterns	0:38	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.	0:143	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	11	43	dep	acquired	2225:2232	arg1	ii					2212:2213	ii	2212:2213	ii	2212:2213	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	2	44	theme	digestive	433:441	arg1	microbiota					443:452	the digestive microbiota	429:452	the digestive microbiota	429:452	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	0	45	theme	algal	13:17	arg1	patterns					31:38	Seasonal and algal diet-driven patterns	0:38	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.	0:143	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	8	46	theme	digestive	1571:1579	arg1	gland					1581:1585	the abalone digestive gland	1559:1585	the abalone digestive gland	1559:1585	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	5	47	from	area	1133:1136	arg1	farmed					1103:1108	farmed	1103:1108	farmed	1103:1108	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	8	48	dep	genera	1471:1476	arg1	Vibrio					1513:1518	Vibrio	1513:1518	Vibrio	1513:1518	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	8	48	dep	genera	1471:1476	arg1	Psychrilyobacter					1479:1494	Psychrilyobacter	1479:1494	Psychrilyobacter	1479:1494	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	8	48	dep	genera	1471:1476	arg1	Mycoplasma					1497:1506	Mycoplasma	1497:1506	Mycoplasma	1497:1506	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	8	49	from	microbiota	1545:1554	arg1	gland					1581:1585	the abalone digestive gland	1559:1585	the abalone digestive gland	1559:1585	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	9	50	theme	abundant	1609:1616	arg1	microbiota					1641:1650	a less abundant and diet-specific core microbiota	1602:1650	a less abundant and diet-specific core microbiota	1602:1650	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	4	51	theme	monospecific	839:850	arg1	diet					858:861	a monospecific algal diet	837:861	a monospecific algal diet	837:861	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	9	52	theme	diet-specific	1622:1634	arg1	microbiota					1641:1650	a less abundant and diet-specific core microbiota	1602:1650	a less abundant and diet-specific core microbiota	1602:1650	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	7	53	theme	multivariate	1282:1293	arg1	analyses					1295:1302	multivariate analyses	1282:1302	multivariate analyses	1282:1302	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	10	54	theme	diet-specific	1920:1932	arg1	community					1939:1947	a less abundant and diet-specific core community	1900:1947	a less abundant and diet-specific core community	1900:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	1	55	theme	BACKGROUND	145:154	arg1	Holobionts					156:165	BACKGROUND Holobionts	145:165	BACKGROUND Holobionts	145:165	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	10	56	theme	abundant	1907:1914	arg1	community					1939:1947	a less abundant and diet-specific core community	1900:1947	a less abundant and diet-specific core community	1900:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	2	57	theme	land	389:392	arg1	plants					394:399	land plants	389:399	land plants	389:399	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	1	58	theme	complex	248:254	arg1	compounds					264:272	complex dietary compounds	248:272	complex dietary compounds for the host	248:285	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	10	59	from	presence	1888:1895	arg1	gland					1834:1838	the digestive gland	1820:1838	the digestive gland of the abalone since its juvenile state	1820:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	11	60	dep	composed	1956:1963	arg1	compared					1993:2000	compared	1993:2000	compared to terrestrial herbivores	1993:2026	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	1	61	theme	compounds	264:272	arg1	degradation					233:243	the degradation	229:243	the degradation of complex dietary compounds for the host	229:285	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	9	62	theme	aerobic	1681:1687	arg1	degraders					1697:1705	aerobic primary degraders	1681:1705	aerobic primary degraders of algal polysaccharides	1681:1730	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	10	63	theme	community	1939:1947	arg1	establishment					1771:1783	the establishment	1767:1783	the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state	1767:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	10	63	theme	community	1939:1947	arg1	presence					1888:1895	the presence	1884:1895	the presence of a less abundant and diet-specific core community	1884:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	3	64	theme	brown	542:546	arg1	macroalgae					548:557	red, green, and brown macroalgae	526:557	red, green, and brown macroalgae	526:557	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	11	65	theme	abalone	2087:2093	arg1	holobiont					2095:2103	the abalone holobiont	2083:2103	the abalone holobiont	2083:2103	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	10	66	theme	core	1801:1804	arg1	microbiota					1806:1815	a persistent core microbiota	1788:1815	a persistent core microbiota in the digestive gland of the abalone since its juvenile state	1788:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	4	67	theme	algal	852:856	arg1	diet					858:861	a monospecific algal diet	837:861	a monospecific algal diet	837:861	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	10	68	from	establishment	1771:1783	arg1	gland					1834:1838	the digestive gland	1820:1838	the digestive gland of the abalone since its juvenile state	1820:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	7	69	theme	specific	1439:1446	arg1	diet					1454:1457	a specific algal diet	1437:1457	a specific algal diet	1437:1457	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	11	70	theme	aerobic	2281:2287	arg1	microbiota					2295:2304	the aerobic algal microbiota	2277:2304	the aerobic algal microbiota	2277:2304	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	3	71	theme	distinct	566:573	arg1	lineages					575:582	three distinct lineages	560:582	three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass	560:688	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	3	72	theme	biomass	682:688	arg1	half					668:671	half	668:671	half of their biomass	668:688	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	0	73	theme	generalist	116:125	arg1	herbivore					134:142	a generalist marine herbivore	114:142	a generalist marine herbivore	114:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	0	73	theme	generalist	116:125	arg1	tuberculata					101:111	the European abalone Haliotis tuberculata	71:111	the European abalone Haliotis tuberculata	71:111	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	5	74	theme	digestive	1035:1043	arg1	microbiota					1045:1054	the digestive microbiota	1031:1054	the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year	1031:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	10	75	theme	digestive	1824:1832	arg1	gland					1834:1838	the digestive gland	1820:1838	the digestive gland of the abalone since its juvenile state	1820:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	1	76	theme	catabolic	200:208	arg1	abilities					210:218	catabolic abilities	200:218	catabolic abilities allowing the degradation of complex dietary compounds for the host	200:285	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
29587830	5	77	theme	herbivore	1079:1087	arg1	microbiota					1045:1054	the digestive microbiota	1031:1054	the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year	1031:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	11	78	theme	terrestrial	2005:2015	arg1	herbivores					2017:2026	terrestrial herbivores	2005:2026	terrestrial herbivores	2005:2026	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	7	79	theme	parameters	1406:1415	arg1	variations					1381:1390	seasonal variations	1372:1390	seasonal variations of contextual parameters	1372:1415	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	10	80	theme	abalone	1847:1853	arg1	gland					1834:1838	the digestive gland	1820:1838	the digestive gland of the abalone since its juvenile state	1820:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	3	81	theme	complex	627:633	arg1	polysaccharides					635:649	complex polysaccharides	627:649	complex polysaccharides	627:649	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	7	82	theme	seasonal	1372:1379	arg1	variations					1381:1390	seasonal variations	1372:1390	seasonal variations of contextual parameters	1372:1415	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	4	83	theme	specific	731:738	arg1	microbiota					750:759	a specific epiphytic microbiota	729:759	a specific epiphytic microbiota	729:759	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	5	84	theme	generalist	1061:1070	arg1	herbivore					1079:1087	a generalist marine herbivore	1059:1087	a generalist marine herbivore	1059:1087	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	84	theme	generalist	1061:1070	arg1	abalone					1094:1100	the abalone	1090:1100	the abalone	1090:1100	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	11	85	from	microbiota	2295:2304	arg1	transfer					2263:2270	gene transfer	2258:2270	gene transfer from the aerobic algal microbiota	2258:2304	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	10	86	from	microbiota	1806:1815	arg1	gland					1834:1838	the digestive gland	1820:1838	the digestive gland of the abalone since its juvenile state	1820:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	6	87	theme	abalone	1175:1181	arg1	gland					1193:1197	the abalone digestive gland	1171:1197	the abalone digestive gland	1171:1197	The microbiota from the abalone digestive gland was sampled every 2 months and explored using metabarcoding.
29587830	0	88	theme	European	75:82	arg1	herbivore					134:142	a generalist marine herbivore	114:142	a generalist marine herbivore	114:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	0	88	theme	European	75:82	arg1	tuberculata					101:111	the European abalone Haliotis tuberculata	71:111	the European abalone Haliotis tuberculata	71:111	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	5	89	theme	temperate	1115:1123	arg1	area					1133:1136	a temperate coastal area	1113:1136	a temperate coastal area over a year	1113:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	5	90	theme	diets	912:916	arg1	effect					884:889	the effect	880:889	the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year	880:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	0	91	theme	Haliotis	92:99	arg1	herbivore					134:142	a generalist marine herbivore	114:142	a generalist marine herbivore	114:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	0	91	theme	Haliotis	92:99	arg1	tuberculata					101:111	the European abalone Haliotis tuberculata	71:111	the European abalone Haliotis tuberculata	71:111	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	7	92	dep	RESULTS	1260:1266	arg1	Diversity					1268:1276	Diversity	1268:1276	Diversity	1268:1276	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	10	93	from	gland	1834:1838	arg1	establishment					1771:1783	the establishment	1767:1783	the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state	1767:1878	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	10	93	from	gland	1834:1838	arg1	presence					1888:1895	the presence	1884:1895	the presence of a less abundant and diet-specific core community	1884:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	4	94	theme	digestive	770:778	arg1	microbiota					780:789	the digestive microbiota	766:789	the digestive microbiota of marine herbivores	766:810	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	3	95	from	composition	612:622	arg1	polysaccharides					635:649	complex polysaccharides	627:649	complex polysaccharides	627:649	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	7	96	theme	microbiota	1332:1341	arg1	patterns					1316:1323	patterns	1316:1323	patterns of the microbiota	1316:1341	RESULTS Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet.
29587830	5	97	from	farmed	1103:1108	arg1	area					1133:1136	a temperate coastal area	1113:1136	a temperate coastal area over a year	1113:1148	We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year.
29587830	8	98	theme	abalone	1563:1569	arg1	gland					1581:1585	the abalone digestive gland	1559:1585	the abalone digestive gland	1559:1585	Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland.
29587830	10	99	dep	CONCLUSIONS	1733:1743	arg1	highlights					1756:1765	highlights	1756:1765	highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community	1756:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	0	100	theme	diet-driven	19:29	arg1	patterns					31:38	Seasonal and algal diet-driven patterns	0:38	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.	0:143	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	11	101	theme	different	1968:1976	arg1	taxa					1988:1991	different microbial taxa	1968:1991	different microbial taxa	1968:1991	While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.
29587830	0	102	theme	digestive	47:55	arg1	microbiota					57:66	the digestive microbiota	43:66	the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore	43:142	Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore.
29587830	2	103	from	plants	394:399	arg1	polysaccharides					368:382	complex polysaccharides	360:382	complex polysaccharides from land plants	360:399	In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized.
29587830	10	104	theme	core	1934:1937	arg1	community					1939:1947	a less abundant and diet-specific core community	1900:1947	a less abundant and diet-specific core community	1900:1947	CONCLUSIONS This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community.
29587830	9	105	theme	core	1636:1639	arg1	microbiota					1641:1650	a less abundant and diet-specific core microbiota	1602:1650	a less abundant and diet-specific core microbiota	1602:1650	Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.
29587830	4	106	theme	herbivores	801:810	arg1	microbiota					780:789	the digestive microbiota	766:789	the digestive microbiota of marine herbivores	766:810	Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet.
29587830	3	107	theme	green	531:535	arg1	macroalgae					548:557	red, green, and brown macroalgae	526:557	red, green, and brown macroalgae	526:557	Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass.
29587830	1	108	theme	dietary	256:262	arg1	compounds					264:272	complex dietary compounds	248:272	complex dietary compounds for the host	248:285	BACKGROUND Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host.
31422177	0	0	theme	clarkia	82:88	arg1	performance					11:21	growth performance	4:21	growth performance	4:21	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	0	0	theme	clarkia	82:88	arg1	immunity					39:46	nonspecific immunity	27:46	nonspecific immunity	27:46	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	4	1	theme	cells	972:976	arg1	efficiency					777:786	feed efficiency	772:786	feed efficiency	772:786	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	activity					818:825	phenoloxidase activity	804:825	phenoloxidase activity	804:825	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	number					915:920	number	915:920	number	915:920	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	level					882:886	glutathione peroxidase level	859:886	glutathione peroxidase level	859:886	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	gain					766:769	body weight gain	754:769	body weight gain	754:769	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	rate					798:801	survival rate	789:801	survival rate	789:801	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	activity					849:856	superoxide dismutase activity	828:856	superoxide dismutase activity	828:856	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	resistance					982:991	resistance	982:991	resistance to Aeromonas hydrophila	982:1015	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	1	theme	cells	972:976	arg1	count					905:909	total haemocyte count	889:909	total haemocyte count	889:909	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	6	2	theme	diets	1235:1239	arg1	intake					1225:1230	intake	1225:1230	intake of diets containing RRP	1225:1254	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	1	3	from	effects	176:182	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	3	from	effects	176:182	arg1	immunity					265:272	nonspecific immunity	253:272	nonspecific immunity	253:272	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	5	4	theme	optimum	1165:1171	arg1	dose					1173:1176	the optimum dose	1161:1176	the optimum dose of RRP	1161:1183	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	4	theme	optimum	1165:1171	arg1	diet-1					1210:1215	0.6 g kg diet-1	1201:1215	0.6 g kg diet-1	1201:1215	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	0	5	theme	Procambarus	70:80	arg1	clarkia					82:88	red swamp crayfish Procambarus clarkia	51:88	red swamp crayfish Procambarus clarkia	51:88	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	2	6	theme	water	341:345	arg1	extraction					347:356	hot water extraction	337:356	hot water extraction	337:356	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	6	7	contain	containing	1241:1250	arg2	RRP					1252:1254	RRP	1252:1254	RRP	1252:1254	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	6	7	contain	containing	1241:1250	arg1	diets					1235:1239	diets	1235:1239	diets containing RRP	1235:1254	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	4	8	theme	cells	933:937	arg1	efficiency					777:786	feed efficiency	772:786	feed efficiency	772:786	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	activity					818:825	phenoloxidase activity	804:825	phenoloxidase activity	804:825	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	number					915:920	number	915:920	number	915:920	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	level					882:886	glutathione peroxidase level	859:886	glutathione peroxidase level	859:886	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	gain					766:769	body weight gain	754:769	body weight gain	754:769	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	rate					798:801	survival rate	789:801	survival rate	789:801	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	activity					849:856	superoxide dismutase activity	828:856	superoxide dismutase activity	828:856	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	resistance					982:991	resistance	982:991	resistance to Aeromonas hydrophila	982:1015	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	8	theme	cells	933:937	arg1	count					905:909	total haemocyte count	889:909	total haemocyte count	889:909	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	3	9	theme	RRP	689:691	arg1	supplementation					693:707	any RRP supplementation	685:707	any RRP supplementation	685:707	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	4	10	theme	peroxidase	871:880	arg1	level					882:886	glutathione peroxidase level	859:886	glutathione peroxidase level	859:886	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	11	theme	phenoloxidase	804:816	arg1	activity					818:825	phenoloxidase activity	804:825	phenoloxidase activity	804:825	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	12	theme	body	754:757	arg1	gain					766:769	body weight gain	754:769	body weight gain	754:769	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	1	13	theme	nonspecific	253:263	arg1	immunity					265:272	nonspecific immunity	253:272	nonspecific immunity	253:272	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	4	14	theme	hyaline	925:931	arg1	cells					933:937	hyaline cells	925:937	hyaline cells	925:937	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	15	theme	glutathione	859:869	arg1	level					882:886	glutathione peroxidase level	859:886	glutathione peroxidase level	859:886	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	5	16	theme	RRP	1181:1183	arg1	dose					1173:1176	the optimum dose	1161:1176	the optimum dose of RRP	1161:1183	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	16	theme	RRP	1181:1183	arg1	diet-1					1210:1215	0.6 g kg diet-1	1201:1215	0.6 g kg diet-1	1201:1215	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	0	17	theme	Rhodiola	110:117	arg1	polysaccharide					125:138	dietary Rhodiola rosea polysaccharide	102:138	dietary Rhodiola rosea polysaccharide	102:138	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	3	18	theme	RRP	513:515	arg1	levels					503:508	three different levels	487:508	three different levels of RRP (0.2, 0.6 and 1 g kg diet-1)	487:544	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	2	19	theme	sugar	429:433	arg1	analyses					447:454	sugar composition analyses	429:454	sugar composition analyses	429:454	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	2	20	theme	liquid	403:408	arg1	chromatography					410:423	high-performance liquid chromatography	386:423	high-performance liquid chromatography	386:423	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	0	21	theme	dietary	102:108	arg1	polysaccharide					125:138	dietary Rhodiola rosea polysaccharide	102:138	dietary Rhodiola rosea polysaccharide	102:138	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	5	22	dep	performance	1111:1121	arg1	the					1100:1102	the	1100:1102	the	1100:1102	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	4	23	theme	weight	759:764	arg1	gain					766:769	body weight gain	754:769	body weight gain	754:769	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	2	24	theme	high-performance	386:401	arg1	chromatography					410:423	high-performance liquid chromatography	386:423	high-performance liquid chromatography	386:423	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	0	25	theme	growth	4:9	arg1	performance					11:21	growth performance	4:21	growth performance	4:21	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	4	26	theme	granular	963:970	arg1	cells					972:976	granular cells	963:976	granular cells	963:976	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	27	theme	dismutase	839:847	arg1	activity					849:856	superoxide dismutase activity	828:856	superoxide dismutase activity	828:856	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	5	28	theme	crayfish	1151:1158	arg1	performance					1111:1121	growth performance	1104:1121	growth performance	1104:1121	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	28	theme	crayfish	1151:1158	arg1	immunity					1139:1146	nonspecific immunity	1127:1146	nonspecific immunity	1127:1146	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	4	29	theme	superoxide	828:837	arg1	activity					849:856	superoxide dismutase activity	828:856	superoxide dismutase activity	828:856	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	6	30	theme	performance	1281:1291	arg1	responses					1301:1309	the growth performance, immune responses	1270:1309	responses	1301:1309	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	1	31	theme	Rhodiola	187:194	arg1	polysaccharide					202:215	Rhodiola rosea polysaccharide	187:215	Rhodiola rosea polysaccharide (RRP)	187:221	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	31	theme	Rhodiola	187:194	arg1	RRP					218:220	RRP	218:220	RRP	218:220	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	3	32	theme	different	493:501	arg1	levels					503:508	three different levels	487:508	three different levels of RRP (0.2, 0.6 and 1 g kg diet-1)	487:544	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	1	33	theme	red	277:279	arg1	clarkia					308:314	red swamp crayfish Procambarus clarkia	277:314	red swamp crayfish Procambarus clarkia	277:314	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	4	34	theme	feeding	745:751	arg1	weeks					736:740	8 weeks	734:740	8 weeks of feeding	734:751	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	1	35	theme	rosea	196:200	arg1	polysaccharide					202:215	Rhodiola rosea polysaccharide	187:215	Rhodiola rosea polysaccharide (RRP)	187:221	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	35	theme	rosea	196:200	arg1	RRP					218:220	RRP	218:220	RRP	218:220	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	36	theme	swamp	281:285	arg1	clarkia					308:314	red swamp crayfish Procambarus clarkia	277:314	red swamp crayfish Procambarus clarkia	277:314	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	0	37	theme	rosea	119:123	arg1	polysaccharide					125:138	dietary Rhodiola rosea polysaccharide	102:138	dietary Rhodiola rosea polysaccharide	102:138	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	3	38	theme	clarkii	655:661	arg1	performance					584:594	growth performance	577:594	growth performance	577:594	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	3	38	theme	clarkii	655:661	arg1	immunity					612:619	nonspecific immunity	600:619	nonspecific immunity	600:619	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	1	39	theme	polysaccharide	202:215	arg1	effects					176:182	the effects	172:182	the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia	172:314	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	40	theme	crayfish	287:294	arg1	clarkia					308:314	red swamp crayfish Procambarus clarkia	277:314	red swamp crayfish Procambarus clarkia	277:314	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	6	41	theme	growth	1274:1279	arg1	responses					1301:1309	the growth performance, immune responses	1270:1309	responses	1301:1309	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	0	42	theme	nonspecific	27:37	arg1	immunity					39:46	nonspecific immunity	27:46	nonspecific immunity	27:46	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	1	43	theme	Procambarus	296:306	arg1	clarkia					308:314	red swamp crayfish Procambarus clarkia	277:314	red swamp crayfish Procambarus clarkia	277:314	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	3	44	theme	swamp	628:632	arg1	clarkii					655:661	red swamp crayfish Procambarus clarkii	624:661	red swamp crayfish Procambarus clarkii	624:661	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	3	45	theme	crayfish	634:641	arg1	clarkii					655:661	red swamp crayfish Procambarus clarkii	624:661	red swamp crayfish Procambarus clarkii	624:661	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	3	46	theme	nonspecific	600:610	arg1	immunity					612:619	nonspecific immunity	600:619	nonspecific immunity	600:619	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	6	47	theme	immune	1294:1299	arg1	responses					1301:1309	the growth performance, immune responses	1270:1309	responses	1301:1309	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	0	48	theme	red	51:53	arg1	clarkia					82:88	red swamp crayfish Procambarus clarkia	51:88	red swamp crayfish Procambarus clarkia	51:88	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	1	49	theme	clarkia	308:314	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	1	49	theme	clarkia	308:314	arg1	immunity					265:272	nonspecific immunity	253:272	nonspecific immunity	253:272	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	3	50	theme	Procambarus	643:653	arg1	clarkii					655:661	red swamp crayfish Procambarus clarkii	624:661	red swamp crayfish Procambarus clarkii	624:661	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	3	51	theme	growth	577:582	arg1	performance					584:594	growth performance	577:594	growth performance	577:594	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	2	52	theme	hot	337:339	arg1	extraction					347:356	hot water extraction	337:356	hot water extraction	337:356	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	5	53	theme	growth	1104:1109	arg1	performance					1111:1121	growth performance	1104:1121	growth performance	1104:1121	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	54	theme	nonspecific	1127:1137	arg1	immunity					1139:1146	nonspecific immunity	1127:1146	nonspecific immunity	1127:1146	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	4	55	theme	cells	953:957	arg1	efficiency					777:786	feed efficiency	772:786	feed efficiency	772:786	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	activity					818:825	phenoloxidase activity	804:825	phenoloxidase activity	804:825	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	number					915:920	number	915:920	number	915:920	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	level					882:886	glutathione peroxidase level	859:886	glutathione peroxidase level	859:886	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	gain					766:769	body weight gain	754:769	body weight gain	754:769	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	rate					798:801	survival rate	789:801	survival rate	789:801	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	activity					849:856	superoxide dismutase activity	828:856	superoxide dismutase activity	828:856	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	resistance					982:991	resistance	982:991	resistance to Aeromonas hydrophila	982:1015	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	55	theme	cells	953:957	arg1	count					905:909	total haemocyte count	889:909	total haemocyte count	889:909	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	56	theme	semigranular	940:951	arg1	cells					953:957	semigranular cells	940:957	semigranular cells	940:957	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	57	theme	haemocyte	895:903	arg1	count					905:909	total haemocyte count	889:909	total haemocyte count	889:909	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	58	theme	feed	772:775	arg1	efficiency					777:786	feed efficiency	772:786	feed efficiency	772:786	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	3	59	theme	red	624:626	arg1	clarkii					655:661	red swamp crayfish Procambarus clarkii	624:661	red swamp crayfish Procambarus clarkii	624:661	Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet-1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control.
31422177	4	60	theme	survival	789:796	arg1	rate					798:801	survival rate	789:801	survival rate	789:801	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	4	61	theme	total	889:893	arg1	count					905:909	total haemocyte count	889:909	total haemocyte count	889:909	After 8 weeks of feeding, body weight gain, feed efficiency, survival rate, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase level, total haemocyte count and number of hyaline cells, semigranular cells and granular cells and resistance to Aeromonas hydrophila were higher than those of the control.
31422177	1	62	dep	performance	237:247	arg1	the					226:228	the	226:228	the	226:228	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31422177	0	63	dep	performance	11:21	arg1	The					0:2	The	0:2	The	0:2	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	0	64	theme	crayfish	61:68	arg1	clarkia					82:88	red swamp crayfish Procambarus clarkia	51:88	red swamp crayfish Procambarus clarkia	51:88	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	6	65	theme	crayfish	1337:1344	arg1	resistance					1323:1332	resistance	1323:1332	resistance of crayfish to infection by A. hydrophila	1323:1374	Hence, intake of diets containing RRP could enhance the growth performance, immune responses and improve resistance of crayfish to infection by A. hydrophila.
31422177	2	66	theme	composition	435:445	arg1	analyses					447:454	sugar composition analyses	429:454	sugar composition analyses	429:454	RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses.
31422177	0	67	theme	swamp	55:59	arg1	clarkia					82:88	red swamp crayfish Procambarus clarkia	51:88	red swamp crayfish Procambarus clarkia	51:88	The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide.
31422177	5	68	theme	RRP	1093:1095	arg1	efficiency					1079:1088	the efficiency	1075:1088	the efficiency of RRP on the growth performance and nonspecific immunity of crayfish	1075:1158	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	69	from	efficiency	1079:1088	arg1	performance					1111:1121	growth performance	1104:1121	growth performance	1104:1121	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	69	from	efficiency	1079:1088	arg1	immunity					1139:1146	nonspecific immunity	1127:1146	nonspecific immunity	1127:1146	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	70	theme	0.6 g kg	1201:1208	arg1	diet-1					1210:1215	0.6 g kg diet-1	1201:1215	0.6 g kg diet-1	1201:1215	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	5	70	theme	0.6 g kg	1201:1208	arg1	dose					1173:1176	the optimum dose	1161:1176	the optimum dose of RRP	1161:1183	Moreover, based on the efficiency of RRP on the growth performance and nonspecific immunity of crayfish, the optimum dose of RRP was found to be 0.6 g kg diet-1.
31422177	1	71	theme	growth	230:235	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia.
31499961	1	0	from	result	325:330	arg1	endothelialization					335:352	endothelialization	335:352	endothelialization of blood vessel	335:368	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	1	theme	great	158:162	arg1	potential					164:172	great potential	158:172	great potential	158:172	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	7	2	attach	attached	1245:1252	arg2	Cells					1239:1243	Cells	1239:1243	Cells	1239:1243	Cells attached tightly to the nanofibers and infiltrated into the materials, forming an interconnected cell community.
31499961	7	2	attach	attached	1245:1252	arg1	nanofibers					1269:1278	the nanofibers	1265:1278	the nanofibers	1265:1278	Cells attached tightly to the nanofibers and infiltrated into the materials, forming an interconnected cell community.
31499961	1	3	theme	blood	357:361	arg1	vessel					363:368	blood vessel	357:368	blood vessel	357:368	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	9	4	theme	osteogenic	1678:1687	arg1	differentiation					1689:1703	the osteogenic differentiation	1674:1703	the osteogenic differentiation	1674:1703	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	8	5	theme	alkaline	1474:1481	arg1	phosphatase					1483:1493	alkaline phosphatase	1474:1493	alkaline phosphatase activity (ALP)	1474:1508	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	1	6	theme	hyaluronic	267:276	arg1	biocompatibility					238:253	biocompatibility	238:253	biocompatibility	238:253	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	6	theme	hyaluronic	267:276	arg1	oHAs					301:304	oHAs	301:304	oHAs	301:304	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	6	theme	hyaluronic	267:276	arg1	oligosaccharides					283:298	hyaluronic acid oligosaccharides	267:298	especially hyaluronic acid oligosaccharides (oHAs)	256:305	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	0	7	theme	acid	111:114	arg1	oligosaccharide					116:130	hyaluronic acid oligosaccharide	100:130	hyaluronic acid oligosaccharide	100:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	1	8	theme	vessel	363:368	arg1	endothelialization					335:352	endothelialization	335:352	endothelialization of blood vessel	335:368	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	4	9	theme	X-Ray	817:821	arg1	XRD					839:841	XRD	839:841	XRD	839:841	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	9	theme	X-Ray	817:821	arg1	diffractometry					823:836	X-Ray diffractometry	817:836	X-Ray diffractometry (XRD)	817:842	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	3	10	from	temperature	708:718	arg1	self-assembly					686:698	self-assembly	686:698	self-assembly at room temperature	686:718	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	1	11	theme	acid	278:281	arg1	biocompatibility					238:253	biocompatibility	238:253	biocompatibility	238:253	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	11	theme	acid	278:281	arg1	oHAs					301:304	oHAs	301:304	oHAs	301:304	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	11	theme	acid	278:281	arg1	oligosaccharides					283:298	hyaluronic acid oligosaccharides	267:298	especially hyaluronic acid oligosaccharides (oHAs)	256:305	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	0	12	theme	hyaluronic	100:109	arg1	acid					111:114	hyaluronic acid	100:114	hyaluronic acid oligosaccharide	100:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	6	13	theme	bone	1217:1220	arg1	MC3T3-E1					1228:1235	MC3T3-E1	1228:1235	MC3T3-E1	1228:1235	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	13	theme	bone	1217:1220	arg1	cell					1222:1225	the mouse parietal bone cell	1198:1225	the mouse parietal bone cell (MC3T3-E1)	1198:1236	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	5	14	theme	bone	1000:1003	arg1	characteristics					973:987	some characteristics	968:987	some characteristics of natural bone both in composition and microstructure	968:1042	They show some characteristics of natural bone both in composition and microstructure.
31499961	8	15	dep	cells	1426:1430	arg1	adhesion					1432:1439	adhesion	1432:1439	adhesion	1432:1439	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	8	15	dep	cells	1426:1430	arg1	cells					1426:1430	cells adhesion and proliferation	1426:1457	cells adhesion and proliferation	1426:1457	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	8	15	dep	cells	1426:1430	arg1	proliferation					1445:1457	proliferation	1445:1457	proliferation	1445:1457	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	2	16	theme	acid	540:543	arg1	Col/oHAs					563:570	Col/oHAs	563:570	Col/oHAs	563:570	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	2	16	theme	acid	540:543	arg1	oligosaccharides					545:560	hyaluronic acid oligosaccharides	529:560	hyaluronic acid oligosaccharides (Col/oHAs)	529:571	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	6	17	theme	culture	1155:1161	arg1	cell					1182:1185	culture artery endothelial cell	1155:1185	culture artery endothelial cell (PIEC)	1155:1192	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	17	theme	culture	1155:1161	arg1	PIEC					1188:1191	PIEC	1188:1191	PIEC	1188:1191	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	4	18	theme	scanning	879:886	arg1	SEM					909:911	SEM	909:911	SEM	909:911	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	18	theme	scanning	879:886	arg1	microscope					897:906	scanning electron microscope	879:906	scanning electron microscope (SEM)	879:912	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	6	19	theme	hybrid	1079:1084	arg1	nanofiber					1049:1057	The nanofiber	1045:1057	The nanofiber	1045:1057	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	19	theme	hybrid	1079:1084	arg1	network					1086:1092	a hybrid network	1077:1092	a hybrid network which bionics extracellular matrix (ECM)	1077:1133	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	7	20	theme	interconnected	1327:1340	arg1	community					1347:1355	an interconnected cell community	1324:1355	an interconnected cell community	1324:1355	Cells attached tightly to the nanofibers and infiltrated into the materials, forming an interconnected cell community.
31499961	2	21	theme	biomimetic	468:477	arg1	network					489:495	a biomimetic nanofiber network	466:495	a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product	466:599	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	1	22	contain	has	154:156	arg2	potential					164:172	great potential	158:172	great potential	158:172	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	22	contain	has	154:156	arg1	HA					150:151	HA	150:151	HA	150:151	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	22	contain	has	154:156	arg1	acid					144:147	Hyaluronic acid	133:147	Hyaluronic acid (HA)	133:152	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	6	23	theme	endothelial	1170:1180	arg1	cell					1182:1185	culture artery endothelial cell	1155:1185	culture artery endothelial cell (PIEC)	1155:1192	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	23	theme	endothelial	1170:1180	arg1	PIEC					1188:1191	PIEC	1188:1191	PIEC	1188:1191	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	9	24	contain	has	1639:1641	arg2	capacity					1655:1662	a promising capacity	1643:1662	a promising capacity to direct the osteogenic differentiation by providing an adaptable environment	1643:1741	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	9	24	contain	has	1639:1641	arg1	composite					1629:1637	the Col/oHAs/HAP composite	1612:1637	the Col/oHAs/HAP composite	1612:1637	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	9	24	contain	has	1639:1641	arg1	candidate					1779:1787	an excellent candidate	1766:1787	an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation	1766:1884	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	1	25	theme	bone	177:180	arg1	engineering					189:199	bone tissue engineering	177:199	bone tissue engineering due to its favorable bioactivity	177:232	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	0	26	theme	in	10:11	arg1	biocompatibility					19:34	in vitro biocompatibility	10:34	in vitro biocompatibility	10:34	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	9	27	theme	adaptable	1721:1729	arg1	environment					1731:1741	an adaptable environment	1718:1741	an adaptable environment	1718:1741	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	8	28	theme	as-prepared	1372:1382	arg1	nanofiber					1384:1392	the as-prepared nanofiber	1368:1392	the as-prepared nanofiber	1368:1392	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	1	29	theme	tissue	182:187	arg1	engineering					189:199	bone tissue engineering	177:199	bone tissue engineering due to its favorable bioactivity	177:232	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	6	30	theme	parietal	1208:1215	arg1	MC3T3-E1					1228:1235	MC3T3-E1	1228:1235	MC3T3-E1	1228:1235	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	30	theme	parietal	1208:1215	arg1	cell					1222:1225	the mouse parietal bone cell	1198:1225	the mouse parietal bone cell (MC3T3-E1)	1198:1236	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	4	31	theme	electron	888:895	arg1	SEM					909:911	SEM	909:911	SEM	909:911	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	31	theme	electron	888:895	arg1	microscope					897:906	scanning electron microscope	879:906	scanning electron microscope (SEM)	879:912	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	3	32	theme	Biomimetically	602:615	arg1	Col/oHAs/HAP					655:666	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	9	33	theme	PIEC	1867:1870	arg1	proliferation					1872:1884	PIEC proliferation	1867:1884	PIEC proliferation	1867:1884	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	4	34	theme	thermogravimetric	845:861	arg1	TGA					873:875	TGA	873:875	TGA	873:875	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	34	theme	thermogravimetric	845:861	arg1	analysis					863:870	thermogravimetric analysis	845:870	thermogravimetric analysis (TGA)	845:876	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	35	dep	fourier	768:774	arg1	transform					776:784	transform	776:784	transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM)	776:955	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	6	36	theme	extracellular	1108:1120	arg1	ECM					1130:1132	ECM	1130:1132	ECM	1130:1132	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	36	theme	extracellular	1108:1120	arg1	matrix					1122:1127	extracellular matrix	1108:1127	extracellular matrix (ECM)	1108:1133	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	4	37	dep	transform	776:784	arg1	infrared					786:793	infrared	786:793	transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM)	776:955	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	6	38	theme	artery	1163:1168	arg1	cell					1182:1185	culture artery endothelial cell	1155:1185	culture artery endothelial cell (PIEC)	1155:1192	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	38	theme	artery	1163:1168	arg1	PIEC					1188:1191	PIEC	1188:1191	PIEC	1188:1191	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	2	39	theme	scaffold	440:447	arg1	effect					398:403	endothelialized effect	382:403	endothelialized effect	382:403	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	2	39	theme	scaffold	440:447	arg1	performance					420:430	osteogenic performance	409:430	osteogenic performance of bone scaffold	409:447	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	0	40	theme	biomimetic	39:48	arg1	materials					76:84	biomimetic mineralized collagen bone materials	39:84	biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide	39:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	8	41	dep	activity	1495:1502	arg1	expression					1532:1541	expression	1532:1541	expression	1532:1541	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	3	42	theme	based	638:642	arg1	Col/oHAs/HAP					655:666	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	2	43	theme	bone	435:438	arg1	scaffold					440:447	bone scaffold	435:447	bone scaffold	435:447	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	9	44	theme	bone	1793:1796	arg1	tissue					1798:1803	bone tissue	1793:1803	bone tissue	1793:1803	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	8	45	theme	phosphatase	1483:1493	arg1	ALP					1505:1507	ALP	1505:1507	ALP	1505:1507	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	8	45	theme	phosphatase	1483:1493	arg1	activity					1495:1502	alkaline phosphatase activity	1474:1502	alkaline phosphatase activity (ALP)	1474:1508	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	3	46	theme	composite	644:652	arg1	Col/oHAs/HAP					655:666	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	9	47	theme	improved	1833:1840	arg1	performance					1842:1852	improved performance	1833:1852	improved performance of promoting PIEC proliferation	1833:1884	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	1	48	theme	favorable	212:220	arg1	bioactivity					222:232	its favorable bioactivity	208:232	its favorable bioactivity	208:232	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	2	49	theme	nanofiber	479:487	arg1	network					489:495	a biomimetic nanofiber network	466:495	a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product	466:599	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	1	50	theme	promising	315:323	arg1	result					325:330	a promising result	313:330	a promising result in endothelialization of blood vessel	313:368	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	4	51	theme	electron	931:938	arg1	microscopy					940:949	transmission electron microscopy	918:949	transmission electron microscopy (TEM)	918:955	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	51	theme	electron	931:938	arg1	TEM					952:954	TEM	952:954	TEM	952:954	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	0	52	theme	bone	71:74	arg1	materials					76:84	biomimetic mineralized collagen bone materials	39:84	biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide	39:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	3	53	theme	mineralized	617:627	arg1	Col/oHAs/HAP					655:666	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	8	54	theme	positive	1554:1561	arg1	responses					1572:1580	positive cellular responses	1554:1580	positive cellular responses	1554:1580	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	1	55	theme	Hyaluronic	133:142	arg1	HA					150:151	HA	150:151	HA	150:151	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	1	55	theme	Hyaluronic	133:142	arg1	acid					144:147	Hyaluronic acid	133:147	Hyaluronic acid (HA)	133:152	Hyaluronic acid (HA) has great potential in bone tissue engineering due to its favorable bioactivity and biocompatibility, especially hyaluronic acid oligosaccharides (oHAs) shows a promising result in endothelialization of blood vessel.
31499961	0	56	theme	mineralized	50:60	arg1	materials					76:84	biomimetic mineralized collagen bone materials	39:84	biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide	39:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	4	57	theme	transmission	918:929	arg1	microscopy					940:949	transmission electron microscopy	918:949	transmission electron microscopy (TEM)	918:955	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	4	57	theme	transmission	918:929	arg1	TEM					952:954	TEM	952:954	TEM	952:954	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
31499961	3	58	theme	Col/oHAs	629:636	arg1	Col/oHAs/HAP					655:666	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP)	602:667	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	7	59	theme	cell	1342:1345	arg1	community					1347:1355	an interconnected cell community	1324:1355	an interconnected cell community	1324:1355	Cells attached tightly to the nanofibers and infiltrated into the materials, forming an interconnected cell community.
31499961	9	60	theme	excellent	1769:1777	arg1	composite					1629:1637	the Col/oHAs/HAP composite	1612:1637	the Col/oHAs/HAP composite	1612:1637	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	9	60	theme	excellent	1769:1777	arg1	candidate					1779:1787	an excellent candidate	1766:1787	an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation	1766:1884	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	9	61	theme	promising	1645:1653	arg1	capacity					1655:1662	a promising capacity	1643:1662	a promising capacity to direct the osteogenic differentiation by providing an adaptable environment	1643:1741	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	2	62	theme	hyaluronic	529:538	arg1	Col/oHAs					563:570	Col/oHAs	563:570	Col/oHAs	563:570	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	2	62	theme	hyaluronic	529:538	arg1	oligosaccharides					545:560	hyaluronic acid oligosaccharides	529:560	hyaluronic acid oligosaccharides (Col/oHAs)	529:571	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	2	63	theme	mineralized	581:591	arg1	product					593:599	its mineralized product	577:599	its mineralized product	577:599	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	3	64	theme	room	703:706	arg1	temperature					708:718	room temperature	703:718	room temperature	703:718	Biomimetically mineralized Col/oHAs based composite (Col/oHAs/HAP) was prepared via self-assembly at room temperature.
31499961	5	65	theme	natural	992:998	arg1	bone					1000:1003	natural bone	992:1003	natural bone	992:1003	They show some characteristics of natural bone both in composition and microstructure.
31499961	5	66	dep	bone	1000:1003	arg1	both					1005:1008	both	1005:1008	both	1005:1008	They show some characteristics of natural bone both in composition and microstructure.
31499961	0	67	dep	in	10:11	arg1	vitro					13:17	vitro	13:17	vitro	13:17	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	8	68	theme	cellular	1563:1570	arg1	responses					1572:1580	positive cellular responses	1554:1580	positive cellular responses	1554:1580	Moreover, the as-prepared nanofiber was found to noticeably enhance cells adhesion and proliferation and upregulate alkaline phosphatase activity (ALP) and osteocalcin (OCN) expression suggesting positive cellular responses.
31499961	2	69	theme	endothelialized	382:396	arg1	effect					398:403	endothelialized effect	382:403	endothelialized effect	382:403	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	2	70	theme	osteogenic	409:418	arg1	performance					420:430	osteogenic performance	409:430	osteogenic performance of bone scaffold	409:447	To improve endothelialized effect and osteogenic performance of bone scaffold, we have created a biomimetic nanofiber network based on collagen modified with hyaluronic acid oligosaccharides (Col/oHAs) and its mineralized product.
31499961	9	71	theme	Col/oHAs/HAP	1616:1627	arg1	composite					1629:1637	the Col/oHAs/HAP composite	1612:1637	the Col/oHAs/HAP composite	1612:1637	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	9	71	theme	Col/oHAs/HAP	1616:1627	arg1	candidate					1779:1787	an excellent candidate	1766:1787	an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation	1766:1884	These results indicated that the Col/oHAs/HAP composite has a promising capacity to direct the osteogenic differentiation by providing an adaptable environment and can be expected as an excellent candidate for bone tissue engineering approaches with improved performance of promoting PIEC proliferation.
31499961	0	72	theme	collagen	62:69	arg1	materials					76:84	biomimetic mineralized collagen bone materials	39:84	biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide	39:130	Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide.
31499961	6	73	theme	mouse	1202:1206	arg1	MC3T3-E1					1228:1235	MC3T3-E1	1228:1235	MC3T3-E1	1228:1235	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	6	73	theme	mouse	1202:1206	arg1	cell					1222:1225	the mouse parietal bone cell	1198:1225	the mouse parietal bone cell (MC3T3-E1)	1198:1236	The nanofiber was fabricated as a hybrid network which bionics extracellular matrix (ECM) and was prepared to culture artery endothelial cell (PIEC) and the mouse parietal bone cell (MC3T3-E1).
31499961	4	74	theme	resultant	725:733	arg1	composites					735:744	The resultant composites	721:744	The resultant composites	721:744	The resultant composites were characterized by fourier transform infrared spectroscopy (FT-IR), X-Ray diffractometry (XRD), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and transmission electron microscopy (TEM).
30948058	5	0	theme	material	1170:1177	arg1	response					1106:1113	a good cellular response	1090:1113	a good cellular response	1090:1113	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	5	0	theme	material	1170:1177	arg1	interactions					1128:1139	cell-matrix interactions	1116:1139	cell-matrix interactions	1116:1139	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	5	0	theme	material	1170:1177	arg1	biocompatibility					1146:1161	biocompatibility	1146:1161	biocompatibility of the material	1146:1177	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	7	1	theme	pure	1580:1583	arg1	scaffold					1594:1601	unmodified pure collagen scaffold	1569:1601	unmodified pure collagen scaffold	1569:1601	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	4	2	theme	chemical	953:960	arg1	crosslinking					962:973	chemical crosslinking	953:973	chemical crosslinking varying from 60 min to more than one year	953:1015	The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year.
30948058	0	3	theme	in	209:210	arg1	preparation					163:173	Scaffold preparation	154:173	Scaffold preparation	154:173	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	3	theme	in	209:210	arg1	evaluation					229:238	in vitro and ex ovo evaluation	209:238	in vitro and ex ovo evaluation	209:238	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	8	4	theme	dermis	1819:1824	arg1	replacement					1826:1836	dermis replacement	1819:1836	dermis replacement	1819:1836	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	7	5	theme	collagen	1496:1503	arg1	scaffolds					1505:1513	pure collagen scaffolds	1491:1513	pure collagen scaffolds	1491:1513	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	6	6	with	combination	1184:1194	arg1	polysaccharides					1221:1235	natural polysaccharides	1213:1235	natural polysaccharides	1213:1235	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	2	7	theme	scaffolds	671:679	arg1	properties					643:652	not only chemo-physical properties	619:652	not only chemo-physical properties of the composite scaffolds	619:679	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	6	8	theme	positive	1261:1268	arg1	effect					1282:1287	a significant positive synergistic effect	1247:1287	a significant positive synergistic effect on cultivation of cells	1247:1311	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	0	9	theme	Scaffold	154:161	arg1	preparation					163:173	Scaffold preparation	154:173	Scaffold preparation	154:173	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	9	theme	Scaffold	154:161	arg1	characterization					191:206	chemo-physical characterization	176:206	chemo-physical characterization	176:206	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	9	theme	Scaffold	154:161	arg1	evaluation					229:238	in vitro and ex ovo evaluation	209:238	in vitro and ex ovo evaluation	209:238	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	6	10	theme	PicoGreen	1344:1352	arg1	method					1354:1359	PicoGreen method	1344:1359	PicoGreen method	1344:1359	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	8	11	with	systems	1746:1752	arg1	loading					1774:1780	low mechanical loading	1759:1780	low mechanical loading in tissue engineering	1759:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	5	12	link	crosslinked	1058:1068	arg1	scaffolds					1070:1078	chemically crosslinked scaffolds	1047:1078	chemically crosslinked scaffolds	1047:1078	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	2	13	theme	collagen	591:598	arg1	scaffolds					600:608	collagen scaffolds	591:608	collagen scaffolds	591:608	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	3	14	theme	porous	801:806	arg1	scaffolds					808:816	porous scaffolds	801:816	porous scaffolds	801:816	Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio.
30948058	6	15	theme	Membrane	1431:1438	arg1	assay					1446:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	0	16	dep	in	209:210	arg1	vitro					212:216	vitro	212:216	vitro	212:216	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	17	dep	properties	643:652	arg1	only					623:626	only	623:626	only	623:626	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	6	18	theme	Chick	1409:1413	arg1	assay					1446:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	6	19	theme	MTS	1330:1332	arg1	assay					1334:1338	MTS assay	1330:1338	MTS assay	1330:1338	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	6	20	theme	ex	1402:1403	arg1	assay					1446:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	5	21	theme	cell-matrix	1116:1126	arg1	interactions					1128:1139	cell-matrix interactions	1116:1139	cell-matrix interactions	1116:1139	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	8	22	theme	successful	1891:1900	arg1	regeneration					1907:1918	successful skin regeneration	1891:1918	successful skin regeneration	1891:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	23	from	cells	1737:1741	arg1	replacement					1826:1836	dermis replacement	1819:1836	dermis replacement	1819:1836	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	23	from	cells	1737:1741	arg1	systems					1746:1752	systems	1746:1752	systems with low mechanical loading in tissue engineering	1746:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	1	24	theme	properties	433:442	arg1	terms					390:394	terms	390:394	terms of chemical, physical and biological properties	390:442	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	4	25	from	resistance	866:875	arg1	water					926:930	water	926:930	water	926:930	The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year.
30948058	6	26	theme	cells	1307:1311	arg1	effect					1282:1287	a significant positive synergistic effect	1247:1287	a significant positive synergistic effect on cultivation of cells	1247:1311	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	0	27	theme	polysaccharides	57:71	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.	0:239	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	28	theme	calcium	533:539	arg1	salt					541:544	calcium salt	533:544	calcium salt	533:544	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	2	29	theme	biological	705:714	arg1	properties					716:725	their biological properties	699:725	their biological properties	699:725	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	1	30	mod	modified	330:337	arg3	lysate					360:365	bovine platelet lysate	344:365	bovine platelet lysate (BPL)	344:371	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	1	30	mod	modified	330:337	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	1	30	mod	modified	330:337	arg3	BPL					368:370	BPL	368:370	BPL	368:370	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	2	31	theme	cellulose	558:566	arg1	salt					541:544	calcium salt	533:544	calcium salt	533:544	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	2	31	theme	cellulose	558:566	arg1	chitosan					488:495	chitosan	488:495	chitosan	488:495	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	2	31	theme	cellulose	558:566	arg1	complex					521:527	chitin/chitosan-glucan complex	498:527	chitin/chitosan-glucan complex	498:527	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	6	32	theme	CAM	1441:1443	arg1	assay					1446:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	8	33	theme	developed	1630:1638	arg1	sponges					1661:1667	the newly developed crosslinked collagen sponges	1620:1667	the newly developed crosslinked collagen sponges involving bioactive additives	1620:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	33	theme	developed	1630:1638	arg1	scaffold					1716:1723	scaffold	1716:1723	scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration	1716:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	0	34	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.	0:239	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	35	theme	antibacterial	453:465	arg1	polysaccharides					467:481	Natural antibacterial polysaccharides	445:481	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds	445:608	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	8	36	theme	collagen	1652:1659	arg1	sponges					1661:1667	the newly developed crosslinked collagen sponges	1620:1667	the newly developed crosslinked collagen sponges involving bioactive additives	1620:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	36	theme	collagen	1652:1659	arg1	scaffold					1716:1723	scaffold	1716:1723	scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration	1716:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	4	37	theme	collagen	880:887	arg1	sponges					889:895	collagen sponges	880:895	collagen sponges	880:895	The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year.
30948058	0	38	theme	platelet	29:36	arg1	lysate					38:43	bovine platelet lysate	22:43	bovine platelet lysate	22:43	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	5	39	theme	cellular	1097:1104	arg1	response					1106:1113	a good cellular response	1090:1113	a good cellular response	1090:1113	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	1	40	theme	bovine	344:349	arg1	BPL					368:370	BPL	368:370	BPL	368:370	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	1	40	theme	bovine	344:349	arg1	lysate					360:365	bovine platelet lysate	344:365	bovine platelet lysate (BPL)	344:371	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	6	41	theme	natural	1213:1219	arg1	polysaccharides					1221:1235	natural polysaccharides	1213:1235	natural polysaccharides	1213:1235	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	8	42	used	used	1708:1711	arg2	scaffold					1716:1723	scaffold	1716:1723	scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration	1716:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	42	used	used	1708:1711	arg2	sponges					1661:1667	the newly developed crosslinked collagen sponges	1620:1667	the newly developed crosslinked collagen sponges involving bioactive additives	1620:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	43	theme	low	1759:1761	arg1	loading					1774:1780	low mechanical loading	1759:1780	low mechanical loading in tissue engineering	1759:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	6	44	theme	synergistic	1270:1280	arg1	effect					1282:1287	a significant positive synergistic effect	1247:1287	a significant positive synergistic effect on cultivation of cells	1247:1311	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	6	45	theme	collagen	1199:1206	arg1	combination					1184:1194	The combination	1180:1194	The combination of collagen with natural polysaccharides	1180:1235	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	1	46	theme	3D	253:254	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	7	47	theme	collagen	1585:1592	arg1	scaffold					1594:1601	unmodified pure collagen scaffold	1569:1601	unmodified pure collagen scaffold	1569:1601	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	3	48	theme	Lipophilic	763:772	arg1	BPL					774:776	Lipophilic BPL	763:776	Lipophilic BPL	763:776	Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio.
30948058	8	49	theme	tissue	1785:1790	arg1	engineering					1792:1802	tissue engineering	1785:1802	tissue engineering	1785:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	1	50	theme	collagen-polysaccharide	263:285	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	7	51	theme	unmodified	1569:1578	arg1	scaffold					1594:1601	unmodified pure collagen scaffold	1569:1601	unmodified pure collagen scaffold	1569:1601	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	2	52	theme	chemo-physical	628:641	arg1	properties					643:652	not only chemo-physical properties	619:652	not only chemo-physical properties of the composite scaffolds	619:679	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	0	53	theme	ex	222:223	arg1	preparation					163:173	Scaffold preparation	154:173	Scaffold preparation	154:173	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	53	theme	ex	222:223	arg1	evaluation					229:238	in vitro and ex ovo evaluation	209:238	in vitro and ex ovo evaluation	209:238	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	4	54	theme	hydrolytic	900:909	arg1	degradation					911:921	hydrolytic degradation	900:921	hydrolytic degradation	900:921	The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year.
30948058	7	55	theme	scaffolds	1505:1513	arg1	modification					1463:1474	modification	1463:1474	modification only by BLP of pure collagen scaffolds	1463:1513	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	0	56	dep	ex	222:223	arg1	ovo					225:227	ovo	225:227	ovo	225:227	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	57	theme	composite	661:669	arg1	scaffolds					671:679	the composite scaffolds	657:679	the composite scaffolds	657:679	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	7	58	theme	pure	1491:1494	arg1	scaffolds					1505:1513	pure collagen scaffolds	1491:1513	pure collagen scaffolds	1491:1513	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	3	59	from	microspheres	785:796	arg1	scaffolds					808:816	porous scaffolds	801:816	porous scaffolds	801:816	Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio.
30948058	0	60	theme	tissue	134:139	arg1	engineering					141:151	tissue engineering	134:151	tissue engineering	134:151	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	1	61	link	Crosslinked	241:251	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	6	62	dep	ex	1402:1403	arg1	ovo					1405:1407	ovo	1405:1407	ovo	1405:1407	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	6	63	theme	Chorioallantoic	1415:1429	arg1	assay					1446:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	ex ovo Chick Chorioallantoic Membrane (CAM) assay	1402:1450	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	1	64	theme	chemical	399:406	arg1	properties					433:442	chemical, physical and biological properties	399:442	chemical, physical and biological properties	399:442	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	3	65	theme	swelling	846:853	arg1	ratio					855:859	their swelling ratio	840:859	their swelling ratio	840:859	Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio.
30948058	1	66	theme	physical	409:416	arg1	properties					433:442	chemical, physical and biological properties	399:442	chemical, physical and biological properties	399:442	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	8	67	theme	crucial	1869:1875	arg1	neovascularization					1845:1862	neovascularization	1845:1862	neovascularization	1845:1862	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	67	theme	crucial	1869:1875	arg1	parameter					1877:1885	a crucial parameter	1867:1885	a crucial parameter for successful skin regeneration	1867:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	0	68	theme	chemo-physical	176:189	arg1	preparation					163:173	Scaffold preparation	154:173	Scaffold preparation	154:173	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	68	theme	chemo-physical	176:189	arg1	characterization					191:206	chemo-physical characterization	176:206	chemo-physical characterization	176:206	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	1	69	theme	biological	422:431	arg1	properties					433:442	chemical, physical and biological properties	399:442	chemical, physical and biological properties	399:442	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	7	70	dep	exhibited	1515:1523	arg1	decreased					1525:1533	decreased	1525:1533	exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold	1515:1601	Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold.
30948058	8	71	theme	skin	1902:1905	arg1	regeneration					1907:1918	successful skin regeneration	1891:1918	successful skin regeneration	1891:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	0	72	theme	biological	80:89	arg1	properties					91:100	the biological properties	76:100	the biological properties of collagen-based scaffolds for tissue engineering	76:151	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	73	from	effect	12:17	arg1	properties					91:100	the biological properties	76:100	the biological properties of collagen-based scaffolds for tissue engineering	76:151	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	5	74	theme	in-vitro	1031:1038	arg1	tests					1040:1044	in-vitro tests	1031:1044	in-vitro tests	1031:1044	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	8	75	from	loading	1774:1780	arg1	engineering					1792:1802	tissue engineering	1785:1802	tissue engineering	1785:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	6	76	from	effect	1282:1287	arg1	cultivation					1292:1302	cultivation	1292:1302	cultivation	1292:1302	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	0	77	theme	scaffolds	120:128	arg1	properties					91:100	the biological properties	76:100	the biological properties of collagen-based scaffolds for tissue engineering	76:151	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	78	theme	oxidized	549:556	arg1	cellulose					558:566	oxidized cellulose	549:566	oxidized cellulose (CaOC) incorporated in collagen scaffolds	549:608	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	2	78	theme	oxidized	549:556	arg1	CaOC					569:572	CaOC	569:572	CaOC	569:572	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	8	79	theme	crosslinked	1640:1650	arg1	sponges					1661:1667	the newly developed crosslinked collagen sponges	1620:1667	the newly developed crosslinked collagen sponges involving bioactive additives	1620:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	79	theme	crosslinked	1640:1650	arg1	scaffold					1716:1723	scaffold	1716:1723	scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration	1716:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	0	80	theme	bovine	22:27	arg1	lysate					38:43	bovine platelet lysate	22:43	bovine platelet lysate	22:43	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	2	81	theme	Natural	445:451	arg1	polysaccharides					467:481	Natural antibacterial polysaccharides	445:481	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds	445:608	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	8	82	theme	bioactive	1679:1687	arg1	additives					1689:1697	bioactive additives	1679:1697	bioactive additives	1679:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	6	83	theme	significant	1249:1259	arg1	effect					1282:1287	a significant positive synergistic effect	1247:1287	a significant positive synergistic effect on cultivation of cells	1247:1311	The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay.
30948058	0	84	theme	lysate	38:43	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.	0:239	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	5	85	theme	good	1092:1095	arg1	response					1106:1113	a good cellular response	1090:1113	a good cellular response	1090:1113	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	2	86	theme	chitin/chitosan-glucan	498:519	arg1	complex					521:527	chitin/chitosan-glucan complex	498:527	chitin/chitosan-glucan complex	498:527	Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented.
30948058	0	87	theme	collagen-based	105:118	arg1	scaffolds					120:128	collagen-based scaffolds	105:128	collagen-based scaffolds for tissue engineering	105:151	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	88	theme	various	49:55	arg1	polysaccharides					57:71	various polysaccharides	49:71	various polysaccharides	49:71	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	4	89	theme	sponges	889:895	arg1	resistance					866:875	The resistance	862:875	The resistance of collagen sponges to hydrolytic degradation in water	862:930	The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year.
30948058	8	90	theme	mechanical	1763:1772	arg1	loading					1774:1780	low mechanical loading	1759:1780	low mechanical loading in tissue engineering	1759:1802	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	1	91	theme	platelet	351:358	arg1	BPL					368:370	BPL	368:370	BPL	368:370	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	1	91	theme	platelet	351:358	arg1	lysate					360:365	bovine platelet lysate	344:365	bovine platelet lysate (BPL)	344:371	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	5	92	theme	crosslinked	1058:1068	arg1	scaffolds					1070:1078	chemically crosslinked scaffolds	1047:1078	chemically crosslinked scaffolds	1047:1078	According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell-matrix interactions, and biocompatibility of the material.
30948058	1	93	theme	Crosslinked	241:251	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30948058	0	94	dep	effect	12:17	arg1	preparation					163:173	Scaffold preparation	154:173	Scaffold preparation	154:173	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	94	dep	effect	12:17	arg1	characterization					191:206	chemo-physical characterization	176:206	chemo-physical characterization	176:206	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	0	94	dep	effect	12:17	arg1	evaluation					229:238	in vitro and ex ovo evaluation	209:238	in vitro and ex ovo evaluation	209:238	Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation.
30948058	8	95	link	crosslinked	1640:1650	arg1	sponges					1661:1667	the newly developed crosslinked collagen sponges	1620:1667	the newly developed crosslinked collagen sponges involving bioactive additives	1620:1697	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	95	link	crosslinked	1640:1650	arg1	scaffold					1716:1723	scaffold	1716:1723	scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration	1716:1918	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	8	96	theme	growing	1729:1735	arg1	cells					1737:1741	growing cells	1729:1741	growing cells	1729:1741	We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
30948058	1	97	theme	porous	256:261	arg1	scaffolds					287:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds	241:295	Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties.
30940591	9	0	theme	LPP	927:929	arg1	viscosities					904:914	The viscosities	900:914	The viscosities of HPP and LPP	900:929	The viscosities of HPP and LPP had different tolerances to acidity and alkalinity.
30940591	3	1	theme	chemical	206:213	arg1	compositions					215:226	The chemical compositions	202:226	The chemical compositions	202:226	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	6	2	theme	proteoglycans	663:675	arg1	viscosity					642:650	the viscosity	638:650	the viscosity of the two proteoglycans	638:675	The temperature had different effects on the viscosity of the two proteoglycans.
30940591	3	3	theme	rheological	313:323	arg1	properties					325:334	rheological properties	313:334	rheological properties	313:334	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	4	theme	proteoglycans	347:359	arg1	properties					325:334	rheological properties	313:334	rheological properties	313:334	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	4	theme	proteoglycans	347:359	arg1	compositions					280:291	monosaccharide compositions	265:291	monosaccharide compositions	265:291	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	4	theme	proteoglycans	347:359	arg1	compositions					215:226	The chemical compositions	202:226	The chemical compositions	202:226	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	4	theme	proteoglycans	347:359	arg1	spectra					300:306	FT-IR spectra	294:306	FT-IR spectra	294:306	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	4	theme	proteoglycans	347:359	arg1	weights					256:262	relative average molecular weights	229:262	relative average molecular weights	229:262	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	13	5	theme	pharmacological	1434:1448	arg1	applications					1450:1461	pharmacological applications	1434:1461	pharmacological applications	1434:1461	The above data presented that the two proteoglycans could be promising candidates for food industries and pharmacological applications.
30940591	9	6	contain	had	931:933	arg2	tolerances					945:954	different tolerances	935:954	different tolerances	935:954	The viscosities of HPP and LPP had different tolerances to acidity and alkalinity.
30940591	9	6	contain	had	931:933	arg1	viscosities					904:914	The viscosities	900:914	The viscosities of HPP and LPP	900:929	The viscosities of HPP and LPP had different tolerances to acidity and alkalinity.
30940591	7	7	dep	85 °C	714:718	arg1	to					711:712	to	711:712	to	711:712	As temperature increased from 25 to 85 °C, the viscosity of LPP descended while the HPP's viscosity rose first and then dropped slightly.
30940591	2	8	theme	different	128:136	arg1	ratios					138:143	different ratios	128:143	different ratios of protein/polysaccharide	128:169	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	12	9	theme	HPP	1149:1151	arg1	modulus					1128:1134	The modulus G' and G″	1124:1144	The modulus G' and G″ of HPP and LPP	1124:1159	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	3	10	theme	average	238:244	arg1	weights					256:262	relative average molecular weights	229:262	relative average molecular weights	229:262	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	3	11	theme	molecular	246:254	arg1	weights					256:262	relative average molecular weights	229:262	relative average molecular weights	229:262	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	2	12	with	proteoglycans	95:107	arg1	ratios					138:143	different ratios	128:143	different ratios of protein/polysaccharide	128:169	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	12	13	theme	LPP	1157:1159	arg1	modulus					1128:1134	The modulus G' and G″	1124:1144	The modulus G' and G″ of HPP and LPP	1124:1159	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	12	14	theme	LPP	1323:1325	arg1	concentrations					1297:1310	the increasing concentrations	1282:1310	the increasing concentrations of HPP and LPP	1282:1325	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	12	15	theme	G″	1257:1258	arg1	values					1260:1265	G' and G″ values	1250:1265	G' and G″ values	1250:1265	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	0	16	theme	Chemical	0:7	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)	0:82	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)
30940591	13	17	theme	above	1332:1336	arg1	data					1338:1341	The above data	1328:1341	The above data	1328:1341	The above data presented that the two proteoglycans could be promising candidates for food industries and pharmacological applications.
30940591	3	18	theme	relative	229:236	arg1	weights					256:262	relative average molecular weights	229:262	relative average molecular weights	229:262	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	4	19	contain	had	427:429	arg1	proteoglycans					413:425	the two proteoglycans	405:425	the two proteoglycans	405:425	The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior.
30940591	4	19	contain	had	427:429	arg2	properties					452:461	pseudoplastic fluids properties	431:461	pseudoplastic fluids properties	431:461	The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior.
30940591	3	20	theme	monosaccharide	265:278	arg1	compositions					280:291	monosaccharide compositions	265:291	monosaccharide compositions	265:291	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	11	21	theme	samples	1115:1121	arg1	viscosities					1100:1110	the viscosities	1096:1110	the viscosities of samples	1096:1121	The addition of sucrose increased the viscosities of samples.
30940591	12	22	theme	crossover	1230:1238	arg1	points					1240:1245	the crossover points	1226:1245	the crossover points of G' and G″ values	1226:1265	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	0	23	theme	rheological	13:23	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)	0:82	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)
30940591	10	24	theme	due	1029:1031	arg1	changes					1021:1027	pH changes	1018:1027	pH changes due to its high protein content	1018:1059	HPP solution was more sensitive to pH changes due to its high protein content.
30940591	13	25	theme	promising	1389:1397	arg1	proteoglycans					1366:1378	the two proteoglycans	1358:1378	the two proteoglycans	1358:1378	The above data presented that the two proteoglycans could be promising candidates for food industries and pharmacological applications.
30940591	13	25	theme	promising	1389:1397	arg1	candidates					1399:1408	promising candidates	1389:1408	promising candidates for food industries and pharmacological applications	1389:1461	The above data presented that the two proteoglycans could be promising candidates for food industries and pharmacological applications.
30940591	0	26	theme	proteoglycans	39:51	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)	0:82	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)
30940591	12	27	theme	oscillation	1197:1207	arg1	frequency					1209:1217	oscillation frequency	1197:1217	oscillation frequency	1197:1217	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	2	28	theme	protein/polysaccharide	148:169	arg1	ratios					138:143	different ratios	128:143	different ratios of protein/polysaccharide	128:169	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	10	29	theme	HPP	983:985	arg1	solution					987:994	HPP solution	983:994	HPP solution	983:994	HPP solution was more sensitive to pH changes due to its high protein content.
30940591	5	30	theme	apparent	506:513	arg1	viscosity					515:523	The apparent viscosity	502:523	The apparent viscosity of the two proteoglycans	502:548	The apparent viscosity of the two proteoglycans both increased with increasing concentrations.
30940591	6	31	theme	different	617:625	arg1	effects					627:633	different effects	617:633	different effects	617:633	The temperature had different effects on the viscosity of the two proteoglycans.
30940591	4	32	theme	shear-thinning	477:490	arg1	behavior					492:499	shear-thinning behavior	477:499	shear-thinning behavior	477:499	The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior.
30940591	12	33	theme	increasing	1286:1295	arg1	concentrations					1297:1310	the increasing concentrations	1282:1310	the increasing concentrations of HPP and LPP	1282:1325	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	5	34	theme	increasing	570:579	arg1	concentrations					581:594	increasing concentrations	570:594	increasing concentrations	570:594	The apparent viscosity of the two proteoglycans both increased with increasing concentrations.
30940591	11	35	theme	sucrose	1078:1084	arg1	addition					1066:1073	The addition	1062:1073	The addition of sucrose	1062:1084	The addition of sucrose increased the viscosities of samples.
30940591	2	36	dep	proteoglycans	95:107	arg1	HPP					110:112	HPP	110:112	HPP	110:112	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	2	36	dep	proteoglycans	95:107	arg1	proteoglycans					95:107	Two proteoglycans	91:107	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide	91:169	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	2	36	dep	proteoglycans	95:107	arg1	LPP					118:120	LPP	118:120	LPP	118:120	Two proteoglycans (HPP and LPP) with different ratios of protein/polysaccharide were extracted from S. glabra.
30940591	0	37	from	glabra	68:73	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)	0:82	Chemical and rheological properties of proteoglycans from Sarcandra glabra (Thunb.)
30940591	12	38	theme	HPP	1315:1317	arg1	concentrations					1297:1310	the increasing concentrations	1282:1310	the increasing concentrations of HPP and LPP	1282:1325	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	12	39	theme	frequency	1209:1217	arg1	increase					1185:1192	the increase	1181:1192	the increase of oscillation frequency	1181:1217	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	13	40	theme	food	1414:1417	arg1	industries					1419:1428	food industries	1414:1428	food industries	1414:1428	The above data presented that the two proteoglycans could be promising candidates for food industries and pharmacological applications.
30940591	8	41	from	effects	820:826	arg1	samples					857:863	the two samples	849:863	the two samples	849:863	The effects of CaCl2 addition on the two samples were like that of the temperature.
30940591	10	42	theme	protein	1045:1051	arg1	content					1053:1059	its high protein content	1036:1059	its high protein content	1036:1059	HPP solution was more sensitive to pH changes due to its high protein content.
30940591	10	43	theme	pH	1018:1019	arg1	changes					1021:1027	pH changes	1018:1027	pH changes due to its high protein content	1018:1059	HPP solution was more sensitive to pH changes due to its high protein content.
30940591	8	44	theme	addition	837:844	arg1	effects					820:826	The effects	816:826	The effects of CaCl2 addition on the two samples	816:863	The effects of CaCl2 addition on the two samples were like that of the temperature.
30940591	10	45	theme	high	1040:1043	arg1	content					1053:1059	its high protein content	1036:1059	its high protein content	1036:1059	HPP solution was more sensitive to pH changes due to its high protein content.
30940591	8	46	theme	CaCl2	831:835	arg1	addition					837:844	CaCl2 addition	831:844	CaCl2 addition	831:844	The effects of CaCl2 addition on the two samples were like that of the temperature.
30940591	3	47	theme	FT-IR	294:298	arg1	spectra					300:306	FT-IR spectra	294:306	FT-IR spectra	294:306	The chemical compositions, relative average molecular weights, monosaccharide compositions, FT-IR spectra, and rheological properties of the two proteoglycans were determined.
30940591	4	48	theme	fluids	445:450	arg1	properties					452:461	pseudoplastic fluids properties	431:461	pseudoplastic fluids properties	431:461	The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior.
30940591	12	49	theme	values	1260:1265	arg1	points					1240:1245	the crossover points	1226:1245	the crossover points of G' and G″ values	1226:1265	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	5	50	theme	proteoglycans	536:548	arg1	viscosity					515:523	The apparent viscosity	502:523	The apparent viscosity of the two proteoglycans	502:548	The apparent viscosity of the two proteoglycans both increased with increasing concentrations.
30940591	9	51	theme	different	935:943	arg1	tolerances					945:954	different tolerances	935:954	different tolerances	935:954	The viscosities of HPP and LPP had different tolerances to acidity and alkalinity.
30940591	9	52	theme	HPP	919:921	arg1	viscosities					904:914	The viscosities	900:914	The viscosities of HPP and LPP	900:929	The viscosities of HPP and LPP had different tolerances to acidity and alkalinity.
30940591	4	53	theme	pseudoplastic	431:443	arg1	properties					452:461	pseudoplastic fluids properties	431:461	pseudoplastic fluids properties	431:461	The results exhibited that the two proteoglycans had pseudoplastic fluids properties and displayed shear-thinning behavior.
30940591	6	54	contain	had	613:615	arg1	temperature					601:611	The temperature	597:611	The temperature	597:611	The temperature had different effects on the viscosity of the two proteoglycans.
30940591	6	54	contain	had	613:615	arg2	effects					627:633	different effects	617:633	different effects	617:633	The temperature had different effects on the viscosity of the two proteoglycans.
30940591	12	55	theme	G	1250:1250	arg1	values					1260:1265	G' and G″ values	1250:1265	G' and G″ values	1250:1265	The modulus G' and G″ of HPP and LPP were increased with the increase of oscillation frequency, while the crossover points of G' and G″ values decreased with the increasing concentrations of HPP and LPP.
30940591	7	56	theme	LPP	738:740	arg1	viscosity					725:733	the viscosity	721:733	the viscosity of LPP	721:740	As temperature increased from 25 to 85 °C, the viscosity of LPP descended while the HPP's viscosity rose first and then dropped slightly.
30940591	7	57	theme	viscosity	768:776	arg1	rose					778:781	the HPP's viscosity rose	758:781	the HPP's viscosity rose	758:781	As temperature increased from 25 to 85 °C, the viscosity of LPP descended while the HPP's viscosity rose first and then dropped slightly.
31125649	8	0	theme	gel	1054:1056	arg1	system					1058:1063	gel system	1054:1063	gel system	1054:1063	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	8	1	theme	increased	1092:1100	arg1	concentration					1078:1090	the concentration increased of SCS	1074:1107	the concentration increased of SCS	1074:1107	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	6	2	theme	scanning	832:839	arg1	microscope					850:859	scanning electron microscope	832:859	scanning electron microscope	832:859	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	5	3	theme	water	695:699	arg1	capacity					709:716	water holding capacity	695:716	water holding capacity	695:716	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	1	4	dep	viscosity	247:255	arg1	the					243:245	the	243:245	the	243:245	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	7	5	theme	gel	896:898	arg1	system					900:905	the gel system	892:905	the gel system	892:905	Finally, the gel mechanism of the gel system was established.
31125649	6	6	theme	system	784:789	arg1	microstructure					754:767	microstructure	754:767	microstructure	754:767	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	6	6	theme	system	784:789	arg1	interaction					738:748	interaction	738:748	interaction	738:748	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	0	7	theme	calcium	75:81	arg1	interactions					59:70	interactions	59:70	interactions	59:70	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	0	7	theme	calcium	75:81	arg1	properties					44:53	gel properties	40:53	gel properties	40:53	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	0	8	from	Effect	0:5	arg1	interactions					59:70	interactions	59:70	interactions	59:70	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	0	8	from	Effect	0:5	arg1	properties					44:53	gel properties	40:53	gel properties	40:53	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	8	9	theme	holding	970:976	arg1	capacity					978:985	water holding capacity	964:985	water holding capacity	964:985	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	7	10	theme	system	900:905	arg1	mechanism					879:887	the gel mechanism	871:887	the gel mechanism of the gel system	871:905	Finally, the gel mechanism of the gel system was established.
31125649	8	11	with	compact	1043:1049	arg1	concentration					1078:1090	the concentration increased of SCS	1074:1107	the concentration increased of SCS	1074:1107	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	9	12	theme	important	1234:1242	arg1	role					1244:1247	an important role	1231:1247	an important role	1231:1247	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	6	13	theme	electron	841:848	arg1	microscope					850:859	scanning electron microscope	832:859	scanning electron microscope	832:859	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	5	14	theme	system	633:638	arg1	properties					607:616	The gel properties	599:616	The gel properties of SCS-SPI gel system	599:638	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	4	15	from	effects	455:461	arg1	interactions					521:532	interactions	521:532	interactions	521:532	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	15	from	effects	455:461	arg1	properties					506:515	gel properties	502:515	gel properties	502:515	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	16	theme	gel	577:579	arg1	interactions					521:532	interactions	521:532	interactions	521:532	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	16	theme	gel	577:579	arg1	properties					506:515	gel properties	502:515	gel properties	502:515	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	0	17	theme	soy	99:101	arg1	gel					119:121	soy protein isolate gel	99:121	soy protein isolate gel	99:121	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	9	18	dep	hydrophobic	1168:1178	arg1	bond					1190:1193	hydrogen bond	1181:1193	hydrogen bond	1181:1193	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	9	18	dep	hydrophobic	1168:1178	arg1	bond					1209:1212	disulfide bond	1199:1212	disulfide bond	1199:1212	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	2	19	theme	solid	364:368	arg1	gel					370:372	an excellent solid gel	351:372	an excellent solid gel	351:372	Normally, individual polysaccharides or proteins do not form an excellent solid gel.
31125649	8	20	theme	SCS	1105:1107	arg1	concentration					1078:1090	the concentration increased of SCS	1074:1107	the concentration increased of SCS	1074:1107	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	5	21	theme	holding	701:707	arg1	capacity					709:716	water holding capacity	695:716	water holding capacity	695:716	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	7	22	theme	gel	875:877	arg1	mechanism					879:887	the gel mechanism	871:887	the gel mechanism of the gel system	871:905	Finally, the gel mechanism of the gel system was established.
31125649	2	23	theme	excellent	354:362	arg1	gel					370:372	an excellent solid gel	351:372	an excellent solid gel	351:372	Normally, individual polysaccharides or proteins do not form an excellent solid gel.
31125649	6	24	theme	protein	809:815	arg1	solubility					817:826	protein solubility	809:826	protein solubility	809:826	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	8	25	theme	water	964:968	arg1	capacity					978:985	water holding capacity	964:985	water holding capacity	964:985	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	9	26	theme	protein	1137:1143	arg1	solubility					1145:1154	protein solubility	1137:1154	protein solubility	1137:1154	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	3	27	theme	composite	386:394	arg1	gels					396:399	composite gels	386:399	composite gels	386:399	Therefore, composite gels have received extensive attention.
31125649	4	28	theme	china	473:477	arg1	SCS					490:492	SCS	490:492	SCS	490:492	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	28	theme	china	473:477	arg1	starch					482:487	Smilax china L. starch	466:487	Smilax china L. starch (SCS)	466:493	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	9	29	theme	gel	1268:1270	arg1	system					1272:1277	the gel system	1264:1277	the gel system	1264:1277	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	8	30	theme	gel	947:949	arg1	strength					951:958	gel strength	947:958	gel strength	947:958	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	1	31	theme	food	167:170	arg1	industry					172:179	the food industry	163:179	the food industry	163:179	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	0	32	theme	china	17:21	arg1	starch					26:31	Smilax china L. starch	10:31	Smilax china L. starch	10:31	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	9	33	theme	solubility	1145:1154	arg1	result					1127:1132	the result	1123:1132	the result of protein solubility	1123:1154	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	0	34	theme	isolate	111:117	arg1	gel					119:121	soy protein isolate gel	99:121	soy protein isolate gel	99:121	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	9	35	theme	hydrophobic	1168:1178	arg1	interaction					1214:1224	hydrophobic, hydrogen bond and disulfide bond interaction	1168:1224	hydrophobic, hydrogen bond and disulfide bond interaction	1168:1224	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	0	36	dep	properties	44:53	arg1	the					36:38	the	36:38	the	36:38	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	4	37	theme	SPI	572:574	arg1	gel					577:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	0	38	theme	Smilax	10:15	arg1	starch					26:31	Smilax china L. starch	10:31	Smilax china L. starch	10:31	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	4	39	theme	gel	502:504	arg1	properties					506:515	gel properties	502:515	gel properties	502:515	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	0	40	theme	protein	103:109	arg1	gel					119:121	soy protein isolate gel	99:121	soy protein isolate gel	99:121	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	9	41	theme	hydrogen	1181:1188	arg1	bond					1190:1193	hydrogen bond	1181:1193	hydrogen bond	1181:1193	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	0	42	theme	starch	26:31	arg1	Effect					0:5	Effect	0:5	Effect of Smilax china L. starch on the gel properties and interactions of calcium	0:81	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	3	43	theme	extensive	415:423	arg1	attention					425:433	extensive attention	415:433	extensive attention	415:433	Therefore, composite gels have received extensive attention.
31125649	6	44	dep	interaction	738:748	arg1	the					734:736	the	734:736	the	734:736	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	1	45	theme	gelling	184:190	arg1	Hydrocolloids					124:136	Hydrocolloids	124:136	Hydrocolloids	124:136	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	45	theme	gelling	184:190	arg1	agents					192:197	gelling agents	184:197	gelling agents	184:197	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	0	46	theme	L.	23:24	arg1	starch					26:31	Smilax china L. starch	10:31	Smilax china L. starch	10:31	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	4	47	theme	starch	482:487	arg1	effects					455:461	the effects	451:461	the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel	451:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	48	theme	L.	479:480	arg1	SCS					490:492	SCS	490:492	SCS	490:492	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	48	theme	L.	479:480	arg1	starch					482:487	Smilax china L. starch	466:487	Smilax china L. starch (SCS)	466:493	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	5	49	theme	gel	603:605	arg1	properties					607:616	The gel properties	599:616	The gel properties of SCS-SPI gel system	599:638	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	4	50	dep	properties	506:515	arg1	the					498:500	the	498:500	the	498:500	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	51	theme	protein	555:561	arg1	gel					577:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	52	theme	Smilax	466:471	arg1	SCS					490:492	SCS	490:492	SCS	490:492	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	4	52	theme	Smilax	466:471	arg1	starch					482:487	Smilax china L. starch	466:487	Smilax china L. starch (SCS)	466:493	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	1	53	theme	foods	283:287	arg1	texture					258:264	texture	258:264	texture	258:264	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	53	theme	foods	283:287	arg1	viscosity					247:255	viscosity	247:255	viscosity	247:255	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	53	theme	foods	283:287	arg1	stability					270:278	stability	270:278	stability	270:278	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	5	54	theme	gel	629:631	arg1	system					633:638	SCS-SPI gel system	621:638	SCS-SPI gel system	621:638	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	6	55	theme	gel	780:782	arg1	system					784:789	SCS-SPI gel system	772:789	SCS-SPI gel system	772:789	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	2	56	theme	individual	300:309	arg1	polysaccharides					311:325	polysaccharides	311:325	polysaccharides	311:325	Normally, individual polysaccharides or proteins do not form an excellent solid gel.
31125649	8	57	theme	system	1058:1063	arg1	compact					1043:1049	compact	1043:1049	compact	1043:1049	Viscosity, elasticity, gel strength and water holding capacity were obviously increased and microstructure become more compact of gel system and with the concentration increased of SCS.
31125649	6	58	theme	SCS-SPI	772:778	arg1	system					784:789	SCS-SPI gel system	772:789	SCS-SPI gel system	772:789	Synchronously, the interaction and microstructure of SCS-SPI gel system were evaluated by protein solubility and scanning electron microscope.
31125649	4	59	theme	soy	551:553	arg1	gel					577:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	5	60	theme	SCS-SPI	621:627	arg1	system					633:638	SCS-SPI gel system	621:638	SCS-SPI gel system	621:638	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	9	61	theme	disulfide	1199:1207	arg1	bond					1209:1212	disulfide bond	1199:1212	disulfide bond	1199:1212	Furthermore, the result of protein solubility showed that hydrophobic, hydrogen bond and disulfide bond interaction play an important role on maintaining the gel system.
31125649	4	62	theme	CaSO4-induced	537:549	arg1	gel					577:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	5	63	theme	gel	678:680	arg1	strength					682:689	gel strength	678:689	gel strength	678:689	The gel properties of SCS-SPI gel system were analyzed by dynamic rheological, gel strength and water holding capacity.
31125649	4	64	theme	isolate	563:569	arg1	gel					577:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	CaSO4-induced soy protein isolate (SPI) gel	537:579	In this study, the effects of Smilax china L. starch (SCS) on the gel properties and interactions of CaSO4-induced soy protein isolate (SPI) gel was investigated.
31125649	0	65	theme	gel	40:42	arg1	properties					44:53	gel properties	40:53	gel properties	40:53	Effect of Smilax china L. starch on the gel properties and interactions of calcium sulfate-induced soy protein isolate gel.
31125649	1	66	used	used	155:158	arg2	agents					192:197	gelling agents	184:197	gelling agents	184:197	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	66	used	used	155:158	arg2	Hydrocolloids					124:136	Hydrocolloids	124:136	Hydrocolloids	124:136	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	66	used	used	155:158	arg2	stabilizers					200:210	stabilizers	200:210	stabilizers	200:210	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	66	used	used	155:158	arg2	thickeners					221:230	food thickeners	216:230	food thickeners	216:230	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	67	theme	food	216:219	arg1	Hydrocolloids					124:136	Hydrocolloids	124:136	Hydrocolloids	124:136	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
31125649	1	67	theme	food	216:219	arg1	thickeners					221:230	food thickeners	216:230	food thickeners	216:230	Hydrocolloids have been widely used in the food industry as gelling agents, stabilizers and food thickeners to improve the viscosity, texture and stability of foods.
30007918	7	0	theme	systemic	1294:1301	arg1	inflammation					1303:1314	systemic inflammation	1294:1314	systemic inflammation	1294:1314	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	11	1	theme	P.	2020:2021	arg1	bacterium					2010:2018	the gut bacterium	2002:2018	the gut bacterium P. goldsteinii	2002:2033	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	1	2	theme	traditional	271:281	arg1	medicine					291:298	traditional Chinese medicine	271:298	traditional Chinese medicine	271:298	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	11	3	theme	novel	2045:2049	arg1	prebiotics					2051:2060	novel prebiotics	2045:2060	novel prebiotics	2045:2060	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	3	4	theme	type	572:575	arg1	diabetes					579:586	type 2 diabetes	572:586	type 2 diabetes	572:586	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	7	5	theme	adipose	1230:1236	arg1	tissues					1238:1244	adipose tissues	1230:1244	adipose tissues	1230:1244	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	1	6	theme	anamorph	212:219	arg1	sinensis					232:239	its anamorph Hirsutella sinensis	208:239	its anamorph Hirsutella sinensis	208:239	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	1	7	from	medicine	291:298	arg1	history					253:259	a long history	246:259	a long history of use in traditional Chinese medicine for their immunomodulatory properties	246:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	5	8	theme	microbiota	867:876	arg1	transplantation					878:892	horizontal faecal microbiota transplantation	849:892	horizontal faecal microbiota transplantation (FMT)	849:898	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	8	theme	microbiota	867:876	arg1	FMT					895:897	FMT	895:897	FMT	895:897	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	6	9	theme	weight	1008:1013	arg1	polysaccharides					1015:1029	high-molecular weight polysaccharides	993:1029	high-molecular weight polysaccharides (>300 kDa)	993:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	9	theme	weight	1008:1013	arg1	>300 kDa					1032:1039	>300 kDa	1032:1039	>300 kDa	1032:1039	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	2	10	theme	type	408:411	arg1	diabetes					415:422	type 2 diabetes	408:422	type 2 diabetes	408:422	Alterations of the gut microbiota have been described in obesity and type 2 diabetes.
30007918	4	11	theme	-fed	667:670	arg1	mice					672:675	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	10	12	theme	oral	1741:1744	arg1	treatment					1746:1754	oral treatment	1741:1754	oral treatment of HFD-fed mice with live P. goldsteinii	1741:1795	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	11	13	theme	CONCLUSIONS	1966:1976	arg1	polysaccharides					1982:1996	CONCLUSIONS HSM polysaccharides	1966:1996	CONCLUSIONS HSM polysaccharides	1966:1996	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	3	14	theme	microbiota	629:638	arg1	composition					606:616	the composition	602:616	the composition of the gut microbiota	602:638	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	9	15	theme	antibiotic	1573:1582	arg1	treatment					1584:1592	antibiotic treatment	1573:1592	antibiotic treatment	1573:1592	FMT combined with antibiotic treatment showed that neomycin-sensitive gut bacteria negatively correlated with obesity traits and were required for H1's anti-obesogenic effects.
30007918	1	16	from	use	264:266	arg1	medicine					291:298	traditional Chinese medicine	271:298	traditional Chinese medicine	271:298	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	6	17	theme	Fraction	970:977	arg1	H1					979:980	RESULTS Fraction H1	962:980	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa)	962:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	8	18	theme	microbiota	1375:1384	arg1	analysis					1386:1393	Gut microbiota analysis	1371:1393	Gut microbiota analysis	1371:1393	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	1	19	from	history	253:259	arg1	medicine					291:298	traditional Chinese medicine	271:298	traditional Chinese medicine	271:298	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	9	20	theme	neomycin-sensitive	1606:1623	arg1	bacteria					1629:1636	neomycin-sensitive gut bacteria	1606:1636	neomycin-sensitive gut bacteria	1606:1636	FMT combined with antibiotic treatment showed that neomycin-sensitive gut bacteria negatively correlated with obesity traits and were required for H1's anti-obesogenic effects.
30007918	4	21	theme	molecular	752:760	arg1	weights					762:768	different molecular weights	742:768	different molecular weights	742:768	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	5	22	theme	HSM	786:788	arg1	effects					775:781	The effects	771:781	The effects of HSM and polysaccharides on the gut microbiota	771:830	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	23	theme	horizontal	849:858	arg1	transplantation					878:892	horizontal faecal microbiota transplantation	849:892	horizontal faecal microbiota transplantation (FMT)	849:898	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	23	theme	horizontal	849:858	arg1	FMT					895:897	FMT	895:897	FMT	895:897	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	1	24	theme	medicinal	163:171	arg1	sinensis					195:202	The medicinal fungus Ophiocordyceps sinensis	159:202	The medicinal fungus Ophiocordyceps sinensis	159:202	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	6	25	theme	weight	1068:1073	arg1	gain					1075:1078	body weight gain	1063:1078	body weight gain (∼50% reduction)	1063:1095	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	25	theme	weight	1068:1073	arg1	reduction					1086:1094	∼50% reduction	1081:1094	∼50% reduction	1081:1094	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	8	26	theme	H1	1409:1410	arg1	polysaccharides					1412:1426	H1 polysaccharides	1409:1426	H1 polysaccharides	1409:1426	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	0	27	theme	anti-obesity	74:85	arg1	effects					87:93	the anti-obesity effects	70:93	the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis	70:146	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	1	28	theme	Ophiocordyceps	180:193	arg1	sinensis					195:202	The medicinal fungus Ophiocordyceps sinensis	159:202	The medicinal fungus Ophiocordyceps sinensis	159:202	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	9	29	theme	obesity	1665:1671	arg1	traits					1673:1678	obesity traits	1665:1678	obesity traits	1665:1678	FMT combined with antibiotic treatment showed that neomycin-sensitive gut bacteria negatively correlated with obesity traits and were required for H1's anti-obesogenic effects.
30007918	8	30	theme	Gut	1371:1373	arg1	analysis					1386:1393	Gut microbiota analysis	1371:1393	Gut microbiota analysis	1371:1393	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	3	31	contain	containing	508:517	arg2	polysaccharides					519:533	polysaccharides	519:533	polysaccharides	519:533	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	3	31	contain	containing	508:517	arg1	fractions					498:506	isolated fractions	489:506	isolated fractions containing polysaccharides	489:533	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	10	32	theme	HFD-fed	1759:1765	arg1	mice					1767:1770	HFD-fed mice	1759:1770	HFD-fed mice	1759:1770	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	0	33	theme	Gut	0:2	arg1	goldsteinii					30:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	11	34	dep	P.	2020:2021	arg1	goldsteinii					2023:2033	goldsteinii	2023:2033	goldsteinii	2023:2033	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	6	35	theme	metabolic	1101:1109	arg1	disorders					1111:1119	metabolic disorders	1101:1119	metabolic disorders	1101:1119	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	8	36	theme	goldsteinii	1479:1489	arg1	growth					1453:1458	the growth	1449:1458	the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice	1449:1552	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	0	37	theme	Parabacteroides	14:28	arg1	goldsteinii					30:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	1	38	theme	immunomodulatory	310:325	arg1	properties					327:336	their immunomodulatory properties	304:336	their immunomodulatory properties	304:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	10	39	theme	P.	1782:1783	arg1	goldsteinii					1785:1795	live P. goldsteinii	1777:1795	live P. goldsteinii	1777:1795	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	7	40	theme	protein	1211:1217	arg1	markers					1219:1225	thermogenesis protein markers	1197:1225	thermogenesis protein markers	1197:1225	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	9	41	theme	anti-obesogenic	1707:1721	arg1	effects					1723:1729	H1's anti-obesogenic effects	1702:1729	H1's anti-obesogenic effects	1702:1729	FMT combined with antibiotic treatment showed that neomycin-sensitive gut bacteria negatively correlated with obesity traits and were required for H1's anti-obesogenic effects.
30007918	6	42	theme	%	1084:1084	arg1	gain					1075:1078	body weight gain	1063:1078	body weight gain (∼50% reduction)	1063:1095	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	42	theme	%	1084:1084	arg1	reduction					1086:1094	∼50% reduction	1081:1094	∼50% reduction	1081:1094	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	10	43	dep	reduced	1797:1803	arg1	enhanced					1877:1884	enhanced	1877:1884	enhanced intestinal integrity	1877:1905	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	0	44	theme	predominant	50:60	arg1	role					62:65	a predominant role	48:65	a predominant role	48:65	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	7	45	theme	lipid	1353:1357	arg1	metabolism					1359:1368	lipid metabolism	1353:1368	lipid metabolism	1353:1368	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	10	46	theme	resistance	1954:1963	arg1	levels					1919:1924	levels	1919:1924	levels of inflammation and insulin resistance	1919:1963	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	1	47	contain	have	241:244	arg1	sinensis					232:239	its anamorph Hirsutella sinensis	208:239	its anamorph Hirsutella sinensis	208:239	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	1	47	contain	have	241:244	arg1	sinensis					195:202	The medicinal fungus Ophiocordyceps sinensis	159:202	The medicinal fungus Ophiocordyceps sinensis	159:202	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	1	47	contain	have	241:244	arg2	history					253:259	a long history	246:259	a long history of use in traditional Chinese medicine for their immunomodulatory properties	246:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	2	48	theme	microbiota	362:371	arg1	Alterations					339:349	Alterations	339:349	Alterations of the gut microbiota	339:371	Alterations of the gut microbiota have been described in obesity and type 2 diabetes.
30007918	1	49	theme	long	248:251	arg1	history					253:259	a long history	246:259	a long history of use in traditional Chinese medicine for their immunomodulatory properties	246:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	6	50	contain	containing	982:991	arg2	>300 kDa					1032:1039	>300 kDa	1032:1039	>300 kDa	1032:1039	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	50	contain	containing	982:991	arg2	polysaccharides					1015:1029	high-molecular weight polysaccharides	993:1029	high-molecular weight polysaccharides (>300 kDa)	993:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	50	contain	containing	982:991	arg1	H1					979:980	RESULTS Fraction H1	962:980	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa)	962:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	10	51	theme	tissue	1855:1860	arg1	thermogenesis					1862:1874	increased adipose tissue thermogenesis	1837:1874	increased adipose tissue thermogenesis	1837:1874	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	6	52	theme	RESULTS	962:968	arg1	H1					979:980	RESULTS Fraction H1	962:980	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa)	962:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	0	53	attach	isolated	114:121	arg2	polysaccharides					98:112	polysaccharides	98:112	polysaccharides isolated from Hirsutella sinensis	98:146	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	0	53	attach	isolated	114:121	arg1	sinensis					139:146	Hirsutella sinensis	128:146	Hirsutella sinensis	128:146	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	4	54	theme	High-fat	648:655	arg1	HFD					663:665	HFD	663:665	HFD	663:665	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	4	54	theme	High-fat	648:655	arg1	diet					657:660	DESIGN High-fat diet	641:660	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	4	55	contain	containing	712:721	arg1	fractions					702:710	fractions	702:710	fractions containing polysaccharides of different molecular weights	702:768	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	4	55	contain	containing	712:721	arg2	polysaccharides					723:737	polysaccharides	723:737	polysaccharides of different molecular weights	723:768	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	10	56	theme	increased	1837:1845	arg1	thermogenesis					1862:1874	increased adipose tissue thermogenesis	1837:1874	increased adipose tissue thermogenesis	1837:1874	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	11	57	used	used	2089:2092	arg2	probiotics					2066:2075	probiotics	2066:2075	probiotics	2066:2075	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	11	57	used	used	2089:2092	arg2	prebiotics					2051:2060	novel prebiotics	2045:2060	novel prebiotics	2045:2060	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	10	58	theme	intestinal	1886:1895	arg1	integrity					1897:1905	intestinal integrity	1886:1905	intestinal integrity	1886:1905	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	1	59	theme	Chinese	283:289	arg1	medicine					291:298	traditional Chinese medicine	271:298	traditional Chinese medicine	271:298	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	5	60	theme	16S	926:928	arg1	analysis					952:959	16S rDNA-based microbiota analysis	926:959	16S rDNA-based microbiota analysis	926:959	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	61	theme	polysaccharides	794:808	arg1	effects					775:781	The effects	771:781	The effects of HSM and polysaccharides on the gut microbiota	771:830	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	1	62	theme	Hirsutella	221:230	arg1	sinensis					232:239	its anamorph Hirsutella sinensis	208:239	its anamorph Hirsutella sinensis	208:239	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	8	63	theme	HFD-fed	1541:1547	arg1	mice					1549:1552	HFD-fed mice	1541:1552	HFD-fed mice	1541:1552	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	5	64	theme	rDNA-based	930:939	arg1	analysis					952:959	16S rDNA-based microbiota analysis	926:959	16S rDNA-based microbiota analysis	926:959	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	65	from	effects	775:781	arg1	microbiota					821:830	the gut microbiota	813:830	the gut microbiota	813:830	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	10	66	theme	insulin	1946:1952	arg1	resistance					1954:1963	insulin resistance	1946:1963	insulin resistance	1946:1963	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	5	67	theme	antibiotic	901:910	arg1	treatment					912:920	antibiotic treatment	901:920	antibiotic treatment	901:920	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	6	68	theme	high-molecular	993:1006	arg1	polysaccharides					1015:1029	high-molecular weight polysaccharides	993:1029	high-molecular weight polysaccharides (>300 kDa)	993:1040	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	68	theme	high-molecular	993:1006	arg1	>300 kDa					1032:1039	>300 kDa	1032:1039	>300 kDa	1032:1039	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	7	69	theme	gut	1256:1258	arg1	integrity					1260:1268	gut integrity	1256:1268	gut integrity	1256:1268	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	10	70	theme	inflammation	1929:1940	arg1	levels					1919:1924	levels	1919:1924	levels of inflammation and insulin resistance	1919:1963	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	5	71	theme	microbiota	941:950	arg1	analysis					952:959	16S rDNA-based microbiota analysis	926:959	16S rDNA-based microbiota analysis	926:959	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	4	72	theme	different	742:750	arg1	weights					762:768	different molecular weights	742:768	different molecular weights	742:768	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	3	73	theme	isolated	489:496	arg1	fractions					498:506	isolated fractions	489:506	isolated fractions containing polysaccharides	489:533	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	11	74	theme	type	2115:2118	arg1	diabetes					2122:2129	type 2 diabetes	2115:2129	type 2 diabetes	2115:2129	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	5	75	theme	faecal	860:865	arg1	transplantation					878:892	horizontal faecal microbiota transplantation	849:892	horizontal faecal microbiota transplantation (FMT)	849:898	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	5	75	theme	faecal	860:865	arg1	FMT					895:897	FMT	895:897	FMT	895:897	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	9	76	theme	gut	1625:1627	arg1	bacteria					1629:1636	neomycin-sensitive gut bacteria	1606:1636	neomycin-sensitive gut bacteria	1606:1636	FMT combined with antibiotic treatment showed that neomycin-sensitive gut bacteria negatively correlated with obesity traits and were required for H1's anti-obesogenic effects.
30007918	7	77	from	expression	1183:1192	arg1	tissues					1238:1244	adipose tissues	1230:1244	adipose tissues	1230:1244	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	1	78	theme	fungus	173:178	arg1	sinensis					195:202	The medicinal fungus Ophiocordyceps sinensis	159:202	The medicinal fungus Ophiocordyceps sinensis	159:202	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	6	79	theme	body	1063:1066	arg1	gain					1075:1078	body weight gain	1063:1078	body weight gain (∼50% reduction)	1063:1095	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	6	79	theme	body	1063:1066	arg1	reduction					1086:1094	∼50% reduction	1081:1094	∼50% reduction	1081:1094	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	0	80	theme	polysaccharides	98:112	arg1	effects					87:93	the anti-obesity effects	70:93	the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis	70:146	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	1	81	dep	OBJECTIVE	149:157	arg1	have					241:244	have	241:244	have a long history of use in traditional Chinese medicine for their immunomodulatory properties	241:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
30007918	6	82	theme	HFD-fed	1124:1130	arg1	mice					1132:1135	HFD-fed mice	1124:1135	HFD-fed mice	1124:1135	RESULTS Fraction H1 containing high-molecular weight polysaccharides (>300 kDa) considerably reduced body weight gain (∼50% reduction) and metabolic disorders in HFD-fed mice.
30007918	10	83	theme	mice	1767:1770	arg1	treatment					1746:1754	oral treatment	1741:1754	oral treatment of HFD-fed mice with live P. goldsteinii	1741:1795	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	0	84	theme	commensal	4:12	arg1	goldsteinii					30:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii	0:40	Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis.
30007918	4	85	theme	weights	762:768	arg1	polysaccharides					723:737	polysaccharides	723:737	polysaccharides of different molecular weights	723:768	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	3	86	theme	H.	458:459	arg1	sinensis					461:468	H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides	458:533	sinensis	461:468	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	7	87	theme	markers	1219:1225	arg1	expression					1183:1192	expression	1183:1192	expression of thermogenesis protein markers in adipose tissues	1183:1244	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	3	88	theme	gut	625:627	arg1	microbiota					629:638	the gut microbiota	621:638	the gut microbiota	621:638	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	8	89	theme	Parabacteroides	1463:1477	arg1	bacterium					1504:1512	a commensal bacterium	1492:1512	a commensal bacterium whose level was reduced in HFD-fed mice	1492:1552	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	8	89	theme	Parabacteroides	1463:1477	arg1	goldsteinii					1479:1489	Parabacteroides goldsteinii	1463:1489	Parabacteroides goldsteinii	1463:1489	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	3	90	theme	diet-induced	547:558	arg1	obesity					560:566	diet-induced obesity	547:566	diet-induced obesity	547:566	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	7	91	theme	thermogenesis	1197:1209	arg1	markers					1219:1225	thermogenesis protein markers	1197:1225	thermogenesis protein markers	1197:1225	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	10	92	theme	live	1777:1780	arg1	goldsteinii					1785:1795	live P. goldsteinii	1777:1795	live P. goldsteinii	1777:1795	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	5	93	theme	gut	817:819	arg1	microbiota					821:830	the gut microbiota	813:830	the gut microbiota	813:830	The effects of HSM and polysaccharides on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT), antibiotic treatment and 16S rDNA-based microbiota analysis.
30007918	11	94	theme	HSM	1978:1980	arg1	polysaccharides					1982:1996	CONCLUSIONS HSM polysaccharides	1966:1996	CONCLUSIONS HSM polysaccharides	1966:1996	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	3	95	dep	sinensis	461:468	arg1	HSM					480:482	HSM	480:482	HSM	480:482	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	3	95	dep	sinensis	461:468	arg1	mycelium					470:477	mycelium	470:477	mycelium (HSM)	470:483	We examined the possibility that H. sinensis mycelium (HSM) and isolated fractions containing polysaccharides may prevent diet-induced obesity and type 2 diabetes by modulating the composition of the gut microbiota.
30007918	8	96	theme	commensal	1494:1502	arg1	bacterium					1504:1512	a commensal bacterium	1492:1512	a commensal bacterium whose level was reduced in HFD-fed mice	1492:1552	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	8	96	theme	commensal	1494:1502	arg1	goldsteinii					1479:1489	Parabacteroides goldsteinii	1463:1489	Parabacteroides goldsteinii	1463:1489	Gut microbiota analysis revealed that H1 polysaccharides selectively promoted the growth of Parabacteroides goldsteinii, a commensal bacterium whose level was reduced in HFD-fed mice.
30007918	11	97	theme	gut	2006:2008	arg1	bacterium					2010:2018	the gut bacterium	2002:2018	the gut bacterium P. goldsteinii	2002:2033	CONCLUSIONS HSM polysaccharides and the gut bacterium P. goldsteinii represent novel prebiotics and probiotics that may be used to treat obesity and type 2 diabetes.
30007918	7	98	theme	insulin	1329:1335	arg1	sensitivity					1337:1347	insulin sensitivity	1329:1347	insulin sensitivity	1329:1347	These effects were associated with increased expression of thermogenesis protein markers in adipose tissues, enhanced gut integrity, reduced intestinal and systemic inflammation and improved insulin sensitivity and lipid metabolism.
30007918	10	99	with	treatment	1746:1754	arg1	goldsteinii					1785:1795	live P. goldsteinii	1777:1795	live P. goldsteinii	1777:1795	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	4	100	theme	DESIGN	641:646	arg1	HFD					663:665	HFD	663:665	HFD	663:665	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	4	100	theme	DESIGN	641:646	arg1	diet					657:660	DESIGN High-fat diet	641:660	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	2	101	theme	gut	358:360	arg1	microbiota					362:371	the gut microbiota	354:371	the gut microbiota	354:371	Alterations of the gut microbiota have been described in obesity and type 2 diabetes.
30007918	4	102	theme	diet	657:660	arg1	mice					672:675	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice	641:675	DESIGN High-fat diet (HFD)-fed mice were treated with HSM or fractions containing polysaccharides of different molecular weights.
30007918	10	103	theme	adipose	1847:1853	arg1	thermogenesis					1862:1874	increased adipose tissue thermogenesis	1837:1874	increased adipose tissue thermogenesis	1837:1874	Notably, oral treatment of HFD-fed mice with live P. goldsteinii reduced obesity and was associated with increased adipose tissue thermogenesis, enhanced intestinal integrity and reduced levels of inflammation and insulin resistance.
30007918	1	104	theme	use	264:266	arg1	history					253:259	a long history	246:259	a long history of use in traditional Chinese medicine for their immunomodulatory properties	246:336	OBJECTIVE The medicinal fungus Ophiocordyceps sinensis and its anamorph Hirsutella sinensis have a long history of use in traditional Chinese medicine for their immunomodulatory properties.
31360991	0	0	theme	chondroitin	111:121	arg1	sulfates					123:130	fucosylated chondroitin sulfates	99:130	fucosylated chondroitin sulfates from sea cucumbers	99:149	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	7	1	theme	sea	1356:1358	arg1	cucumbers					1360:1368	these different sea cucumbers	1340:1368	these different sea cucumbers	1340:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	1	2	from	cucumbers	201:209	arg1	FCSs					186:189	FCSs	186:189	FCSs	186:189	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	2	from	cucumbers	201:209	arg1	sulfates					176:183	Fucosylated chondroitin sulfates	152:183	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers	152:209	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	8	3	theme	rich	1416:1419	arg1	analysis					1441:1448	rich detailed structural analysis	1416:1448	rich detailed structural analysis	1416:1448	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	0	4	theme	fucosylated	99:109	arg1	sulfates					123:130	fucosylated chondroitin sulfates	99:130	fucosylated chondroitin sulfates from sea cucumbers	99:149	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	5	5	from	differences	1038:1048	arg1	fucosylation					1053:1064	fucosylation	1053:1064	fucosylation (including its sulfation)	1053:1090	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	3	6	theme	mass	588:591	arg1	FTMS					607:610	FTMS	607:610	FTMS	607:610	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	6	theme	mass	588:591	arg1	spectrometry					593:604	mass spectrometry	588:604	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	7	7	theme	structural	1303:1312	arg1	model					1314:1318	a new structural model	1297:1318	a new structural model for FCS chains from these different sea cucumbers	1297:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	5	8	dep	fucosylation	1053:1064	arg1	including					1067:1075	including	1067:1075	including its sulfation	1067:1089	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	5	9	theme	different	1098:1106	arg1	species					1108:1114	different species	1098:1114	different species of sea cucumbers	1098:1131	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	6	10	theme	Detailed	1134:1141	arg1	analysis					1143:1150	Detailed analysis	1134:1150	Detailed analysis of FTMS ion peaks	1134:1168	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	7	11	theme	different	1346:1354	arg1	cucumbers					1360:1368	these different sea cucumbers	1340:1368	these different sea cucumbers	1340:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	8	12	from	control	1566:1572	arg1	oligosaccharides					1599:1614	their oligosaccharides	1593:1614	FCSs as well as their oligosaccharides	1577:1614	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	8	12	from	control	1566:1572	arg1	FCSs					1577:1580	FCSs	1577:1580	FCSs as well as their oligosaccharides	1577:1614	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	8	13	dep	detailed	1421:1428	arg1	structural					1430:1439	structural	1430:1439	structural	1430:1439	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	3	14	theme	hydrophilic	531:541	arg1	chromatography-Fourier					555:576	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	15	theme	interaction	543:553	arg1	chromatography-Fourier					555:576	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	5	16	contain	had	974:976	arg1	fragments					957:965	The quantified fragments	942:965	The quantified fragments	942:965	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	5	16	contain	had	974:976	arg2	compositions					1002:1013	trisaccharide-repeating compositions	978:1013	trisaccharide-repeating compositions	978:1013	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	0	17	gly	fucosylated	99:109	arg1	sulfates					123:130	fucosylated chondroitin sulfates	99:130	fucosylated chondroitin sulfates from sea cucumbers	99:149	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	1	18	theme	sea	197:199	arg1	cucumbers					201:209	sea cucumbers	197:209	sea cucumbers	197:209	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	0	19	theme	sea	137:139	arg1	cucumbers					141:149	sea cucumbers	137:149	sea cucumbers	137:149	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	8	20	theme	high	1493:1496	arg1	accuracy					1498:1505	high accuracy	1493:1505	high accuracy	1493:1505	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	3	21	dep	chromatography-Fourier	555:576	arg1	FTMS					607:610	FTMS	607:610	FTMS	607:610	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	21	dep	chromatography-Fourier	555:576	arg1	spectrometry					593:604	mass spectrometry	588:604	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	21	dep	chromatography-Fourier	555:576	arg1	transform					578:586	transform	578:586	transform	578:586	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	6	22	theme	polysaccharides	1237:1251	arg1	analysis					1143:1150	Detailed analysis	1134:1150	Detailed analysis of FTMS ion peaks	1134:1168	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	6	22	theme	polysaccharides	1237:1251	arg1	spectroscopy					1210:1221	top-down nuclear magnetic resonance spectroscopy	1174:1221	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	5	23	theme	trisaccharide-repeating	978:1000	arg1	compositions					1002:1013	trisaccharide-repeating compositions	978:1013	trisaccharide-repeating compositions	978:1013	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	6	24	theme	FCS	1233:1235	arg1	polysaccharides					1237:1251	native FCS polysaccharides	1226:1251	native FCS polysaccharides	1226:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	4	25	theme	depolymerized	835:847	arg1	fragments					853:861	These depolymerized FCS fragments	829:861	These depolymerized FCS fragments	829:861	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	2	26	theme	structure-activity	423:440	arg1	relationship					442:453	their structure-activity relationship	417:453	their structure-activity relationship in the development of new anticoagulant drugs	417:499	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	0	27	theme	Bottom-up	0:8	arg1	analysis					10:17	Bottom-up analysis	0:17	Bottom-up analysis	0:17	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	8	28	with	information	1476:1486	arg1	reproducibility					1511:1525	reproducibility	1511:1525	reproducibility	1511:1525	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	8	28	with	information	1476:1486	arg1	accuracy					1498:1505	high accuracy	1493:1505	high accuracy	1493:1505	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	3	29	theme	FCS	640:642	arg1	oligosaccharides					644:659	the FCS oligosaccharides	636:659	the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides	636:826	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	1	30	theme	repetitive	216:225	arg1	structures					227:236	repetitive structures	216:236	repetitive structures that exhibit minor structural differences	216:278	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	0	31	theme	liquid	25:30	arg1	spectrometry					70:81	liquid chromatography-Fourier transform mass spectrometry	25:81	liquid chromatography-Fourier transform mass spectrometry	25:81	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	8	32	theme	quantitative	1463:1474	arg1	information					1476:1486	quantitative information	1463:1486	quantitative information with high accuracy and reproducibility	1463:1525	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	2	33	theme	detailed	335:342	arg1	important					394:402	important	394:402	important	394:402	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	2	33	theme	detailed	335:342	arg1	characterization					344:359	A detailed characterization	333:359	A detailed characterization of FCSs and their derivatives	333:389	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	2	34	theme	new	477:479	arg1	drugs					495:499	new anticoagulant drugs	477:499	new anticoagulant drugs	477:499	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	2	35	theme	anticoagulant	481:493	arg1	drugs					495:499	new anticoagulant drugs	477:499	new anticoagulant drugs	477:499	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	8	36	theme	quality	1558:1564	arg1	control					1566:1572	the quality control	1554:1572	the quality control in FCSs as well as their oligosaccharides	1554:1614	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	5	37	theme	sea	1119:1121	arg1	cucumbers					1123:1131	sea cucumbers	1119:1131	sea cucumbers	1119:1131	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	3	38	theme	cucumbers	721:729	arg1	badionotus					745:754	Isostichopus badionotus	732:754	Isostichopus badionotus	732:754	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	38	theme	cucumbers	721:729	arg1	graeffei					772:779	Pearsonothuria graeffei	757:779	Pearsonothuria graeffei	757:779	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	38	theme	cucumbers	721:729	arg1	mexicana					793:800	Holothuria mexicana	782:800	Holothuria mexicana	782:800	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	38	theme	cucumbers	721:729	arg1	molpadioides					815:826	Acaudina molpadioides	806:826	Acaudina molpadioides	806:826	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	38	theme	cucumbers	721:729	arg1	species					706:712	four species	701:712	four species of sea cucumbers	701:729	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	4	39	theme	FCS	849:851	arg1	fragments					853:861	These depolymerized FCS fragments	829:861	These depolymerized FCS fragments	829:861	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	5	40	theme	quantified	946:955	arg1	fragments					957:965	The quantified fragments	942:965	The quantified fragments	942:965	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	6	41	theme	method	1283:1288	arg1	accuracy					1266:1273	the accuracy	1262:1273	the accuracy of this method	1262:1288	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	1	42	contain	have	211:214	arg1	FCSs					186:189	FCSs	186:189	FCSs	186:189	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	42	contain	have	211:214	arg1	sulfates					176:183	Fucosylated chondroitin sulfates	152:183	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers	152:209	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	42	contain	have	211:214	arg2	structures					227:236	repetitive structures	216:236	repetitive structures that exhibit minor structural differences	216:278	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	0	43	dep	spectrometry	70:81	arg1	transform					55:63	transform	55:63	transform	55:63	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	8	44	theme	bottom-up	1388:1396	arg1	approach					1398:1405	This bottom-up approach	1383:1405	This bottom-up approach	1383:1405	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	6	45	theme	nuclear	1183:1189	arg1	resonance					1200:1208	top-down nuclear magnetic resonance	1174:1208	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	0	46	theme	chromatography-Fourier	32:53	arg1	spectrometry					70:81	liquid chromatography-Fourier transform mass spectrometry	25:81	liquid chromatography-Fourier transform mass spectrometry	25:81	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	7	47	theme	new	1299:1301	arg1	model					1314:1318	a new structural model	1297:1318	a new structural model for FCS chains from these different sea cucumbers	1297:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	2	48	theme	drugs	495:499	arg1	development					462:472	the development	458:472	the development of new anticoagulant drugs	458:499	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	6	49	theme	magnetic	1191:1198	arg1	resonance					1200:1208	top-down nuclear magnetic resonance	1174:1208	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	1	50	theme	minor	251:255	arg1	differences					268:278	minor structural differences	251:278	minor structural differences	251:278	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	6	51	theme	top-down	1174:1181	arg1	resonance					1200:1208	top-down nuclear magnetic resonance	1174:1208	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	3	52	theme	sea	717:719	arg1	cucumbers					721:729	sea cucumbers	717:729	sea cucumbers	717:729	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	2	53	theme	derivatives	379:389	arg1	important					394:402	important	394:402	important	394:402	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	2	53	theme	derivatives	379:389	arg1	characterization					344:359	A detailed characterization	333:359	A detailed characterization of FCSs and their derivatives	333:389	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	0	54	theme	mass	65:68	arg1	spectrometry					70:81	liquid chromatography-Fourier transform mass spectrometry	25:81	liquid chromatography-Fourier transform mass spectrometry	25:81	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	0	55	from	cucumbers	141:149	arg1	sulfates					123:130	fucosylated chondroitin sulfates	99:130	fucosylated chondroitin sulfates from sea cucumbers	99:149	Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers.
31360991	1	56	theme	Fucosylated	152:162	arg1	FCSs					186:189	FCSs	186:189	FCSs	186:189	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	56	theme	Fucosylated	152:162	arg1	sulfates					176:183	Fucosylated chondroitin sulfates	152:183	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers	152:209	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	6	57	theme	peaks	1164:1168	arg1	analysis					1143:1150	Detailed analysis	1134:1150	Detailed analysis of FTMS ion peaks	1134:1168	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	6	57	theme	peaks	1164:1168	arg1	spectroscopy					1210:1221	top-down nuclear magnetic resonance spectroscopy	1174:1221	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	7	58	from	cucumbers	1360:1368	arg1	chains					1328:1333	FCS chains	1324:1333	FCS chains from these different sea cucumbers	1324:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	3	59	theme	selective	674:682	arg1	degradation					684:694	selective degradation	674:694	selective degradation	674:694	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	2	60	from	relationship	442:453	arg1	development					462:472	the development	458:472	the development of new anticoagulant drugs	458:499	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	1	61	theme	chondroitin	164:174	arg1	FCSs					186:189	FCSs	186:189	FCSs	186:189	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	61	theme	chondroitin	164:174	arg1	sulfates					176:183	Fucosylated chondroitin sulfates	152:183	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers	152:209	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	1	62	theme	structural	257:266	arg1	differences					268:278	minor structural differences	251:278	minor structural differences	251:278	Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered.
31360991	5	63	theme	cucumbers	1123:1131	arg1	species					1108:1114	different species	1098:1114	different species of sea cucumbers	1098:1131	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	2	64	theme	FCSs	364:367	arg1	important					394:402	important	394:402	important	394:402	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	2	64	theme	FCSs	364:367	arg1	characterization					344:359	A detailed characterization	333:359	A detailed characterization of FCSs and their derivatives	333:389	A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs.
31360991	3	65	theme	online	524:529	arg1	chromatography-Fourier					555:576	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS)	524:611	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	3	66	theme	current	509:515	arg1	study					517:521	the current study	505:521	the current study	505:521	In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides.
31360991	4	67	theme	software	912:919	arg1	GlycReSoft					930:939	GlycReSoft	930:939	GlycReSoft	930:939	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	4	67	theme	software	912:919	arg1	package					921:927	the glycomics software package	898:927	the glycomics software package	898:927	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	6	68	theme	resonance	1200:1208	arg1	spectroscopy					1210:1221	top-down nuclear magnetic resonance spectroscopy	1174:1221	top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides	1174:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	6	69	theme	native	1226:1231	arg1	polysaccharides					1237:1251	native FCS polysaccharides	1226:1251	native FCS polysaccharides	1226:1251	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	6	70	theme	ion	1160:1162	arg1	peaks					1164:1168	FTMS ion peaks	1155:1168	FTMS ion peaks	1155:1168	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
31360991	8	71	theme	detailed	1421:1428	arg1	analysis					1441:1448	rich detailed structural analysis	1416:1448	rich detailed structural analysis	1416:1448	This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.
31360991	4	72	theme	glycomics	902:910	arg1	GlycReSoft					930:939	GlycReSoft	930:939	GlycReSoft	930:939	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	4	72	theme	glycomics	902:910	arg1	package					921:927	the glycomics software package	898:927	the glycomics software package	898:927	These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft.
31360991	5	73	theme	significant	1026:1036	arg1	differences					1038:1048	significant differences	1026:1048	significant differences in fucosylation (including its sulfation) among different species of sea cucumbers	1026:1131	The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers.
31360991	7	74	theme	FCS	1324:1326	arg1	chains					1328:1333	FCS chains	1324:1333	FCS chains from these different sea cucumbers	1324:1368	Thus, a new structural model for FCS chains from these different sea cucumbers was defined.
31360991	6	75	theme	FTMS	1155:1158	arg1	peaks					1164:1168	FTMS ion peaks	1155:1168	FTMS ion peaks	1155:1168	Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method.
29454058	6	0	contain	possessed	812:820	arg2	biocompatibility					864:879	good biocompatibility	859:879	good biocompatibility	859:879	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	6	0	contain	possessed	812:820	arg1	sponges					804:810	CMKGM/CS composite sponges	785:810	CMKGM/CS composite sponges	785:810	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	6	0	contain	possessed	812:820	arg2	WVTR					850:853	proper WVTR	843:853	proper WVTR	843:853	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	6	0	contain	possessed	812:820	arg2	ratio					836:840	high swelling ratio	822:840	high swelling ratio	822:840	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	4	1	theme	sponge	496:501	arg1	structure					469:477	the pore structure	460:477	the pore structure of the composite sponge	460:501	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	7	2	theme	male	1061:1064	arg1	mice					1070:1073	male ICR mice	1061:1073	male ICR mice	1061:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	5	3	theme	composite	599:607	arg1	sponges					609:615	CMKGM/CS composite sponges	590:615	CMKGM/CS composite sponges	590:615	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	7	4	dep	in	937:938	arg1	vivo					940:943	vivo	940:943	vivo	940:943	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	6	5	theme	swelling	827:834	arg1	ratio					836:840	high swelling ratio	822:840	high swelling ratio	822:840	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	5	6	theme	water	659:663	arg1	dressing					626:633	wound dressing	620:633	wound dressing	620:633	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	6	theme	water	659:663	arg1	WVTR					690:693	WVTR	690:693	WVTR	690:693	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	6	theme	water	659:663	arg1	rate					684:687	water vapor transmission rate	659:687	water vapor transmission rate (WVTR)	659:694	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	7	theme	sponges	609:615	arg1	applicability					573:585	the applicability	569:585	the applicability of CMKGM/CS composite sponges	569:615	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	2	8	theme	CMKGM/CS	275:282	arg1	sponges					294:300	CMKGM/CS composite sponges	275:300	CMKGM/CS composite sponges	275:300	Then CMKGM and chitosan (CS) were crosslinked and freeze-dried to prepare CMKGM/CS composite sponges with different proportions.
29454058	2	9	theme	composite	284:292	arg1	sponges					294:300	CMKGM/CS composite sponges	275:300	CMKGM/CS composite sponges	275:300	Then CMKGM and chitosan (CS) were crosslinked and freeze-dried to prepare CMKGM/CS composite sponges with different proportions.
29454058	3	10	theme	CMKGM/CS	367:374	arg1	sponges					376:382	CMKGM/CS sponges	367:382	CMKGM/CS sponges	367:382	The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM.
29454058	5	11	theme	vapor	665:669	arg1	dressing					626:633	wound dressing	620:633	wound dressing	620:633	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	11	theme	vapor	665:669	arg1	WVTR					690:693	WVTR	690:693	WVTR	690:693	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	11	theme	vapor	665:669	arg1	rate					684:687	water vapor transmission rate	659:687	water vapor transmission rate (WVTR)	659:694	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	12	theme	wound	620:624	arg1	dressing					626:633	wound dressing	620:633	wound dressing	620:633	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	12	theme	wound	620:624	arg1	biocompatibility					697:712	biocompatibility	697:712	biocompatibility (cytotoxicity and hemolysis)	697:741	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	12	theme	wound	620:624	arg1	rate					684:687	water vapor transmission rate	659:687	water vapor transmission rate (WVTR)	659:694	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	12	theme	wound	620:624	arg1	behavior					649:656	the swelling behavior	636:656	the swelling behavior	636:656	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	1	13	theme	carboxyl	156:163	arg1	modification					165:176	carboxyl modification	156:176	carboxyl modification of konjac glucomannan	156:198	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	4	14	theme	composite	486:494	arg1	sponge					496:501	the composite sponge	482:501	the composite sponge	482:501	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	6	15	theme	CMKGM/CS	785:792	arg1	sponges					804:810	CMKGM/CS composite sponges	785:810	CMKGM/CS composite sponges	785:810	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	0	16	theme	konjac	14:19	arg1	glucomannan					21:31	Carboxymethyl konjac glucomannan	0:31	Carboxymethyl konjac glucomannan	0:31	Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing.
29454058	7	17	theme	in	937:938	arg1	experiments					945:955	in vivo experiments	937:955	in vivo experiments	937:955	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	2	18	theme	different	307:315	arg1	proportions					317:327	different proportions	307:327	different proportions	307:327	Then CMKGM and chitosan (CS) were crosslinked and freeze-dried to prepare CMKGM/CS composite sponges with different proportions.
29454058	0	19	theme	Carboxymethyl	0:12	arg1	glucomannan					21:31	Carboxymethyl konjac glucomannan	0:31	Carboxymethyl konjac glucomannan	0:31	Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing.
29454058	5	20	theme	swelling	640:647	arg1	dressing					626:633	wound dressing	620:633	wound dressing	620:633	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	20	theme	swelling	640:647	arg1	behavior					649:656	the swelling behavior	636:656	the swelling behavior	636:656	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	1	21	theme	konjac	181:186	arg1	glucomannan					188:198	konjac glucomannan	181:198	konjac glucomannan	181:198	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	3	22	theme	sponges	376:382	arg1	micromorphology					348:362	micromorphology	348:362	micromorphology	348:362	The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM.
29454058	3	22	theme	sponges	376:382	arg1	structure					334:342	structure	334:342	structure	334:342	The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM.
29454058	4	23	theme	content	550:556	arg1	increase					532:539	the increase	528:539	the increase of CMKGM content	528:556	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	0	24	link	crosslinked	35:45	arg1	chitosan					47:54	crosslinked chitosan	35:54	crosslinked chitosan	35:54	Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing.
29454058	6	25	theme	high	822:825	arg1	ratio					836:840	high swelling ratio	822:840	high swelling ratio	822:840	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	1	26	theme	glucomannan	188:198	arg1	modification					165:176	carboxyl modification	156:176	carboxyl modification of konjac glucomannan	156:198	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	1	27	theme	carboxymethyl	99:111	arg1	CMKGM					133:137	CMKGM	133:137	CMKGM	133:137	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	1	27	theme	carboxymethyl	99:111	arg1	glucomannan					120:130	carboxymethyl konjac glucomannan	99:130	carboxymethyl konjac glucomannan (CMKGM)	99:138	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	7	28	theme	mice	1070:1073	arg1	defects					1050:1056	skin defects	1045:1056	skin defects of male ICR mice	1045:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	6	29	theme	good	859:862	arg1	biocompatibility					864:879	good biocompatibility	859:879	good biocompatibility	859:879	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	0	30	theme	crosslinked	35:45	arg1	chitosan					47:54	crosslinked chitosan	35:54	crosslinked chitosan	35:54	Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing.
29454058	3	31	dep	structure	334:342	arg1	The					330:332	The	330:332	The	330:332	The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM.
29454058	4	32	theme	pore	464:467	arg1	structure					469:477	the pore structure	460:477	the pore structure of the composite sponge	460:501	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	3	33	theme	FTIR	405:408	arg1	spectroscopy					410:421	FTIR spectroscopy	405:421	FTIR spectroscopy	405:421	The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM.
29454058	5	34	theme	transmission	671:682	arg1	dressing					626:633	wound dressing	620:633	wound dressing	620:633	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	34	theme	transmission	671:682	arg1	WVTR					690:693	WVTR	690:693	WVTR	690:693	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	34	theme	transmission	671:682	arg1	rate					684:687	water vapor transmission rate	659:687	water vapor transmission rate (WVTR)	659:694	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	7	35	theme	skin	1045:1048	arg1	defects					1050:1056	skin defects	1045:1056	skin defects of male ICR mice	1045:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	1	36	theme	konjac	113:118	arg1	CMKGM					133:137	CMKGM	133:137	CMKGM	133:137	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	1	36	theme	konjac	113:118	arg1	glucomannan					120:130	carboxymethyl konjac glucomannan	99:130	carboxymethyl konjac glucomannan (CMKGM)	99:138	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	6	37	theme	tissue	905:910	arg1	regeneration					912:923	tissue regeneration	905:923	tissue regeneration	905:923	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	4	38	theme	CMKGM	544:548	arg1	content					550:556	CMKGM content	544:556	CMKGM content	544:556	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	7	39	theme	ICR	1066:1068	arg1	mice					1070:1073	male ICR mice	1061:1073	male ICR mice	1061:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	6	40	theme	proper	843:848	arg1	WVTR					850:853	proper WVTR	843:853	proper WVTR	843:853	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	7	41	theme	full-layer	1025:1034	arg1	wound					1036:1040	full-layer wound	1025:1040	full-layer wound of skin defects of male ICR mice	1025:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	5	42	theme	CMKGM/CS	590:597	arg1	sponges					609:615	CMKGM/CS composite sponges	590:615	CMKGM/CS composite sponges	590:615	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	7	43	theme	defects	1050:1056	arg1	wound					1036:1040	full-layer wound	1025:1040	full-layer wound of skin defects of male ICR mice	1025:1073	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	7	44	theme	composite	984:992	arg1	sponges					994:1000	CMKGM/CS composite sponges	975:1000	CMKGM/CS composite sponges	975:1000	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	1	45	mod	modification	165:176	arg3	carboxyl					156:163	carboxyl modification	156:176	carboxyl modification of konjac glucomannan	156:198	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	1	45	mod	modification	165:176	arg1	glucomannan					188:198	konjac glucomannan	181:198	konjac glucomannan	181:198	In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan.
29454058	6	46	theme	composite	794:802	arg1	sponges					804:810	CMKGM/CS composite sponges	785:810	CMKGM/CS composite sponges	785:810	The results indicated that CMKGM/CS composite sponges possessed high swelling ratio, proper WVTR and good biocompatibility, which might accelerate tissue regeneration.
29454058	5	47	dep	biocompatibility	697:712	arg1	hemolysis					732:740	hemolysis	732:740	hemolysis	732:740	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	5	47	dep	biocompatibility	697:712	arg1	cytotoxicity					715:726	cytotoxicity	715:726	cytotoxicity	715:726	To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed.
29454058	4	48	theme	SEM	436:438	arg1	results					440:446	The SEM results	432:446	The SEM results	432:446	The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content.
29454058	7	49	theme	CMKGM/CS	975:982	arg1	sponges					994:1000	CMKGM/CS composite sponges	975:1000	CMKGM/CS composite sponges	975:1000	Meanwhile, in vivo experiments demonstrated that CMKGM/CS composite sponges could effectively heal full-layer wound of skin defects of male ICR mice.
29454058	0	50	theme	wound	68:72	arg1	dressing					74:81	wound dressing	68:81	wound dressing	68:81	Carboxymethyl konjac glucomannan - crosslinked chitosan sponges for wound dressing.
31026132	6	0	theme	LUB	1259:1261	arg1	adhesion					1247:1254	the adhesion	1243:1254	the adhesion of LUB to HA	1243:1267	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	6	0	theme	LUB	1259:1261	arg1	strong					1272:1277	strong	1272:1277	strong	1272:1277	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	6	1	from	domains	1359:1365	arg1	molecules					1376:1384	these molecules	1370:1384	these molecules	1370:1384	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	5	2	theme	microscopy	968:977	arg1	measurements					998:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	5	3	theme	composite	1063:1071	arg1	layer					1073:1077	the HA/LUB composite layer	1052:1077	the HA/LUB composite layer	1052:1077	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	1	4	from	surfaces	179:186	arg1	adsorption					123:132	the unwanted adsorption	110:132	the unwanted adsorption of proteins and cells at articular cartilage surfaces	110:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	5	5	theme	layer	1073:1077	arg1	architecture					1036:1047	the architecture	1032:1047	the architecture of the HA/LUB composite layer	1032:1077	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	2	6	theme	thin	362:365	arg1	layer					380:384	a thin, interfacial layer	360:384	a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties	360:588	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	4	7	located	observed	878:885	arg2	enhancement					835:845	A clear synergistic enhancement	815:845	A clear synergistic enhancement in antiadhesive properties	815:872	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	4	7	located	observed	878:885	arg1	films					904:908	the composite films	890:908	the composite films relative to grafted HA and LUB layers alone	890:952	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	3	8	theme	antiadhesive	712:723	arg1	properties					725:734	the antiadhesive properties	708:734	the antiadhesive properties	708:734	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	4	9	theme	composite	894:902	arg1	films					904:908	the composite films	890:908	the composite films relative to grafted HA and LUB layers alone	890:952	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	5	10	theme	force	992:996	arg1	measurements					998:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	2	11	theme	co-adsorbed	505:515	arg1	film					528:531	a co-adsorbed, composite film	503:531	a co-adsorbed, composite film now known to exhibit synergistic tribological properties	503:588	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	6	12	theme	hydrophobic	1301:1311	arg1	coupling					1313:1320	the hydrophobic coupling	1297:1320	the hydrophobic coupling of LUB to HA	1297:1333	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	5	13	theme	strong	1095:1100	arg1	contribution					1102:1113	a strong contribution	1093:1113	a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains	1093:1197	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	5	14	theme	hydrophobic	1118:1128	arg1	interactions					1130:1141	hydrophobic interactions	1118:1141	hydrophobic interactions	1118:1141	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	4	15	theme	antiadhesive	850:861	arg1	properties					863:872	antiadhesive properties	850:872	antiadhesive properties	850:872	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	5	16	theme	normal	985:990	arg1	measurements					998:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	1	17	theme	critical	196:203	arg1	role					205:208	a critical role	194:208	a critical role	194:208	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	5	18	theme	interactions	1130:1141	arg1	contribution					1102:1113	a strong contribution	1093:1113	a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains	1093:1197	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	2	19	dep	thin	362:365	arg1	interfacial					368:378	interfacial	368:378	interfacial	368:378	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	4	20	theme	relative	910:917	arg1	films					904:908	the composite films	890:908	the composite films relative to grafted HA and LUB layers alone	890:952	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	5	21	theme	HA	1189:1190	arg1	chains					1192:1197	HA chains	1189:1197	HA chains	1189:1197	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	2	22	dep	LUB	460:462	arg1	PRG4					472:475	a.k.a. PRG4	465:475	LUB; a.k.a. PRG4	460:475	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	1	23	theme	unwanted	114:121	arg1	adsorption					123:132	the unwanted adsorption	110:132	the unwanted adsorption of proteins and cells at articular cartilage surfaces	110:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	5	24	from	contribution	1102:1113	arg1	binding					1150:1156	the binding	1146:1156	the binding of LUB end-domains directly to HA chains	1146:1197	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	3	25	theme	surface	756:762	arg1	resonance					772:780	surface plasmon resonance	756:780	surface plasmon resonance	756:780	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	2	26	dep	co-adsorbed	505:515	arg1	composite					518:526	composite	518:526	composite	518:526	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	2	27	theme	articular	337:345	arg1	cartilage					347:355	healthy articular cartilage	329:355	healthy articular cartilage	329:355	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	2	28	theme	cartilage	347:355	arg1	surface					318:324	the surface	314:324	the surface of healthy articular cartilage	314:355	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	5	29	theme	Atomic	955:960	arg1	AFM					980:982	AFM	980:982	AFM	980:982	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	5	29	theme	Atomic	955:960	arg1	microscopy					968:977	Atomic force microscopy	955:977	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	2	30	theme	healthy	329:335	arg1	cartilage					347:355	healthy articular cartilage	329:355	healthy articular cartilage	329:355	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	1	31	theme	healthy	225:231	arg1	joints					233:238	healthy joints	225:238	healthy joints	225:238	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	4	32	theme	synergistic	823:833	arg1	enhancement					835:845	A clear synergistic enhancement	815:845	A clear synergistic enhancement in antiadhesive properties	815:872	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	0	33	theme	Hyaluronic	34:43	arg1	Acid					45:48	Hyaluronic Acid	34:48	Hyaluronic Acid	34:48	Interactions between Lubricin and Hyaluronic Acid Synergistically Enhance Antiadhesive Properties.
31026132	5	34	theme	LUB	1161:1163	arg1	end-domains					1165:1175	LUB end-domains	1161:1175	LUB end-domains	1161:1175	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	6	35	theme	LUB	1325:1327	arg1	coupling					1313:1320	the hydrophobic coupling	1297:1320	the hydrophobic coupling of LUB to HA	1297:1333	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	4	36	theme	clear	817:821	arg1	enhancement					835:845	A clear synergistic enhancement	815:845	A clear synergistic enhancement in antiadhesive properties	815:872	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	6	37	theme	other	1409:1413	arg1	proteins					1415:1422	other proteins	1409:1422	other proteins	1409:1422	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	3	38	theme	quartz	786:791	arg1	microbalance					801:812	quartz crystal microbalance	786:812	quartz crystal microbalance	786:812	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	3	39	theme	composite	645:653	arg1	layers					655:660	composite layers	645:660	composite layers of HA and LUB	645:674	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	4	40	from	enhancement	835:845	arg1	properties					863:872	antiadhesive properties	850:872	antiadhesive properties	850:872	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	1	41	theme	proteins	137:144	arg1	adsorption					123:132	the unwanted adsorption	110:132	the unwanted adsorption of proteins and cells at articular cartilage surfaces	110:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	2	42	theme	a.k.a.	465:470	arg1	PRG4					472:475	a.k.a. PRG4	465:475	LUB; a.k.a. PRG4	460:475	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	3	43	theme	plasmon	764:770	arg1	resonance					772:780	surface plasmon resonance	756:780	surface plasmon resonance	756:780	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	2	44	theme	tribological	566:577	arg1	properties					579:588	synergistic tribological properties	554:588	synergistic tribological properties	554:588	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	3	45	theme	LUB	672:674	arg1	layers					655:660	composite layers	645:660	composite layers of HA and LUB	645:674	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	6	46	with	interactions	1391:1402	arg1	proteins					1415:1422	other proteins	1409:1422	other proteins	1409:1422	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	6	46	with	interactions	1391:1402	arg1	molecules					1427:1435	molecules	1427:1435	molecules	1427:1435	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	2	47	theme	synergistic	554:564	arg1	properties					579:588	synergistic tribological properties	554:588	synergistic tribological properties	554:588	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	5	48	theme	HA/LUB	1056:1061	arg1	layer					1073:1077	the HA/LUB composite layer	1052:1077	the HA/LUB composite layer	1052:1077	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	3	49	theme	cartilage	625:633	arg1	surfaces					635:642	cartilage surfaces	625:642	cartilage surfaces	625:642	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	1	50	theme	cells	150:154	arg1	adsorption					123:132	the unwanted adsorption	110:132	the unwanted adsorption of proteins and cells at articular cartilage surfaces	110:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	5	51	theme	force	962:966	arg1	AFM					980:982	AFM	980:982	AFM	980:982	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	5	51	theme	force	962:966	arg1	microscopy					968:977	Atomic force microscopy	955:977	Atomic force microscopy (AFM) normal force measurements	955:1009	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31026132	4	52	theme	grafted	922:928	arg1	HA					930:931	grafted HA	922:931	grafted HA	922:931	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	3	53	theme	HA	665:666	arg1	layers					655:660	composite layers	645:660	composite layers of HA and LUB	645:674	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	3	54	theme	surfaces	635:642	arg1	composition					610:620	the composition	606:620	the composition of cartilage surfaces	606:642	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	1	55	theme	cartilage	169:177	arg1	surfaces					179:186	articular cartilage surfaces	159:186	articular cartilage surfaces	159:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	6	56	theme	hydrophobic	1347:1357	arg1	domains					1359:1365	the hydrophobic domains	1343:1365	the hydrophobic domains in these molecules	1343:1384	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	1	57	theme	degenerative	253:264	arg1	diseases					266:273	degenerative diseases	253:273	degenerative diseases such as osteoarthritis	253:296	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	1	57	theme	degenerative	253:264	arg1	osteoarthritis					283:296	osteoarthritis	283:296	osteoarthritis	283:296	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	4	58	theme	LUB	937:939	arg1	layers					941:946	LUB layers	937:946	LUB layers	937:946	A clear synergistic enhancement in antiadhesive properties was observed in the composite films relative to grafted HA and LUB layers alone.
31026132	6	59	theme	force	1210:1214	arg1	measurements					1216:1227	These AFM force measurements	1200:1227	These AFM force measurements	1200:1227	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	1	60	theme	articular	159:167	arg1	surfaces					179:186	articular cartilage surfaces	159:186	articular cartilage surfaces	159:186	Preventing the unwanted adsorption of proteins and cells at articular cartilage surfaces plays a critical role in maintaining healthy joints and avoiding degenerative diseases such as osteoarthritis.
31026132	2	61	theme	hyaluronic	425:434	arg1	HA					442:443	HA	442:443	HA	442:443	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	2	61	theme	hyaluronic	425:434	arg1	acid					436:439	hyaluronic acid	425:439	hyaluronic acid (HA)	425:444	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	3	62	theme	crystal	793:799	arg1	microbalance					801:812	quartz crystal microbalance	786:812	quartz crystal microbalance	786:812	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	0	63	theme	Antiadhesive	74:85	arg1	Properties					87:96	Antiadhesive Properties	74:96	Antiadhesive Properties	74:96	Interactions between Lubricin and Hyaluronic Acid Synergistically Enhance Antiadhesive Properties.
31026132	3	64	theme	Au	692:693	arg1	surfaces					695:702	Au surfaces	692:702	Au surfaces	692:702	Bioinspired by the composition of cartilage surfaces, composite layers of HA and LUB were grafted to Au surfaces and the antiadhesive properties were assessed using surface plasmon resonance and quartz crystal microbalance.
31026132	6	65	theme	AFM	1206:1208	arg1	measurements					1216:1227	These AFM force measurements	1200:1227	These AFM force measurements	1200:1227	These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules.
31026132	2	66	theme	macromolecules	389:402	arg1	layer					380:384	a thin, interfacial layer	360:384	a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties	360:588	Immobilized at the surface of healthy articular cartilage is a thin, interfacial layer of macromolecules consisting mainly of hyaluronic acid (HA) and lubricin (LUB; a.k.a. PRG4) that is believed to form a co-adsorbed, composite film now known to exhibit synergistic tribological properties.
31026132	5	67	theme	end-domains	1165:1175	arg1	binding					1150:1156	the binding	1146:1156	the binding of LUB end-domains directly to HA chains	1146:1197	Atomic force microscopy (AFM) normal force measurements provide insight into the architecture of the HA/LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end-domains directly to HA chains.
31390269	0	0	theme	Gut	73:75	arg1	Microecology					77:88	Gut Microecology	73:88	Gut Microecology in Mice	73:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	0	1	from	Effects	15:21	arg1	Leaf					65:68	Moringa oleifera Leaf	48:68	Moringa oleifera Leaf	48:68	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	0	1	from	Effects	15:21	arg1	Microecology					77:88	Gut Microecology	73:88	Gut Microecology in Mice	73:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	6	2	theme	intervention	639:650	arg1	weeks					622:626	4 weeks	620:626	4 weeks of feeding intervention with the polysaccharide	620:674	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	3	theme	digestive	783:791	arg1	enzymes					793:799	digestive enzymes	783:799	digestive enzymes	783:799	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	4	theme	serum	819:823	arg1	factor-alpha					840:851	serum tumor necrosis factor-alpha	819:851	serum tumor necrosis factor-alpha	819:851	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	7	5	theme	antiobesity	1028:1038	arg1	effects					1040:1046	antiobesity effects	1028:1046	antiobesity effects	1028:1046	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	5	6	theme	ICR	585:587	arg1	mice					590:593	Institute of Cancer Research (ICR) mice	555:593	Institute of Cancer Research (ICR) mice	555:593	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	1	7	theme	high	140:143	arg1	value					177:181	high nutritional and pharmacological value	140:181	high nutritional and pharmacological value	140:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	5	8	theme	intestinal	524:533	arg1	microenvironment					535:550	intestinal microenvironment	524:550	intestinal microenvironment	524:550	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	6	9	with	intervention	639:650	arg1	polysaccharide					661:674	the polysaccharide	657:674	the polysaccharide	657:674	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	1	10	with	plant	129:133	arg1	value					177:181	high nutritional and pharmacological value	140:181	high nutritional and pharmacological value	140:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	6	11	theme	ICR	728:730	arg1	mice					732:735	the ICR mice	724:735	the ICR mice	724:735	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	8	12	theme	promising	1191:1199	arg1	polysaccharide					1145:1158	the polysaccharide	1141:1158	the polysaccharide of M. oleifera leaf	1141:1178	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	8	12	theme	promising	1191:1199	arg1	prebiotic					1201:1209	a promising prebiotic	1189:1209	a promising prebiotic that exhibits health promotion effects	1189:1248	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	6	13	theme	feeding	631:637	arg1	intervention					639:650	feeding intervention	631:650	feeding intervention with the polysaccharide	631:674	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	3	14	theme	M.	320:321	arg1	leaf					332:335	M. oleifera leaf	320:335	M. oleifera leaf	320:335	In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da).
31390269	3	14	theme	M.	320:321	arg1	MOs-2-a					345:351	MOs-2-a	345:351	MOs-2-a (1.35 × 104 Da)	345:367	In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da).
31390269	5	15	theme	Institute	555:563	arg1	mice					590:593	Institute of Cancer Research (ICR) mice	555:593	Institute of Cancer Research (ICR) mice	555:593	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	7	16	from	composition	929:939	arg1	mice					968:971	mice	968:971	mice	968:971	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	8	17	theme	oleifera	1166:1173	arg1	leaf					1175:1178	M. oleifera leaf	1163:1178	M. oleifera leaf	1163:1178	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	1	18	theme	nutritional	145:155	arg1	value					177:181	high nutritional and pharmacological value	140:181	high nutritional and pharmacological value	140:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	3	19	theme	oleifera	323:330	arg1	leaf					332:335	M. oleifera leaf	320:335	M. oleifera leaf	320:335	In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da).
31390269	3	19	theme	oleifera	323:330	arg1	MOs-2-a					345:351	MOs-2-a	345:351	MOs-2-a (1.35 × 104 Da)	345:367	In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da).
31390269	4	20	theme	ICR	443:445	arg1	mice					447:450	6 week-old ICR mice	432:450	6 week-old ICR mice	432:450	In this study, this polysaccharide was administrated daily to 6 week-old ICR mice for 4 weeks.
31390269	5	21	theme	of	565:566	arg1	mice					590:593	Institute of Cancer Research (ICR) mice	555:593	Institute of Cancer Research (ICR) mice	555:593	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	7	22	theme	acid	1095:1098	arg1	production					1100:1109	lactic acid production	1088:1109	lactic acid production	1088:1109	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	0	23	theme	Beneficial	4:13	arg1	Effects					15:21	The Beneficial Effects	0:21	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice	0:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	4	24	theme	week-old	434:441	arg1	mice					447:450	6 week-old ICR mice	432:450	6 week-old ICR mice	432:450	In this study, this polysaccharide was administrated daily to 6 week-old ICR mice for 4 weeks.
31390269	5	25	theme	Cancer	568:573	arg1	mice					590:593	Institute of Cancer Research (ICR) mice	555:593	Institute of Cancer Research (ICR) mice	555:593	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	5	26	theme	intestinal	498:507	arg1	digestion					509:517	intestinal digestion	498:517	intestinal digestion	498:517	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	1	27	theme	pharmacological	161:175	arg1	value					177:181	high nutritional and pharmacological value	140:181	high nutritional and pharmacological value	140:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	7	28	theme	lactic	1088:1093	arg1	production					1100:1109	lactic acid production	1088:1109	lactic acid production	1088:1109	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	5	29	theme	Research	575:582	arg1	mice					590:593	Institute of Cancer Research (ICR) mice	555:593	Institute of Cancer Research (ICR) mice	555:593	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	6	30	theme	digestive	703:711	arg1	ability					713:719	the immune and intestinal digestive ability	677:719	the immune and intestinal digestive ability of the ICR mice	677:735	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	30	theme	digestive	703:711	arg1	significant					742:752	significant	742:752	significant	742:752	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	8	31	theme	leaf	1175:1178	arg1	polysaccharide					1145:1158	the polysaccharide	1141:1158	the polysaccharide of M. oleifera leaf	1141:1178	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	8	31	theme	leaf	1175:1178	arg1	prebiotic					1201:1209	a promising prebiotic	1189:1209	a promising prebiotic that exhibits health promotion effects	1189:1248	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	8	32	theme	M.	1163:1164	arg1	leaf					1175:1178	M. oleifera leaf	1163:1178	M. oleifera leaf	1163:1178	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	3	33	theme	previous	253:260	arg1	research					262:269	our previous research	249:269	our previous research	249:269	In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da).
31390269	6	34	theme	intestinal	692:701	arg1	ability					713:719	the immune and intestinal digestive ability	677:719	the immune and intestinal digestive ability of the ICR mice	677:735	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	34	theme	intestinal	692:701	arg1	significant					742:752	significant	742:752	significant	742:752	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	35	dep	factor-alpha	840:851	arg1	levels					873:878	levels	873:878	levels	873:878	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	7	36	theme	fatty	1061:1065	arg1	acid					1067:1070	chain fatty acid	1055:1070	short chain fatty acid production	1049:1081	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	0	37	theme	Polysaccharide	28:41	arg1	Effects					15:21	The Beneficial Effects	0:21	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice	0:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	6	38	theme	oxidase	865:871	arg1	index					776:780	the organ index	766:780	the organ index	766:780	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	38	theme	oxidase	865:871	arg1	enzymes					793:799	digestive enzymes	783:799	digestive enzymes	783:799	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	38	theme	oxidase	865:871	arg1	reduction					806:814	reduction	806:814	reduction of serum tumor necrosis factor-alpha and diamine oxidase levels	806:878	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	8	39	theme	promotion	1232:1240	arg1	effects					1242:1248	health promotion effects	1225:1248	health promotion effects	1225:1248	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
31390269	7	40	theme	short	1049:1053	arg1	production					1072:1081	short chain fatty acid production	1049:1081	short chain fatty acid production	1049:1081	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	7	41	theme	acid	1067:1070	arg1	production					1072:1081	short chain fatty acid production	1049:1081	short chain fatty acid production	1049:1081	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	6	42	theme	diamine	857:863	arg1	oxidase					865:871	diamine oxidase	857:871	diamine oxidase	857:871	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	0	43	from	Leaf	65:68	arg1	Effects					15:21	The Beneficial Effects	0:21	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice	0:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	0	43	from	Leaf	65:68	arg1	Polysaccharide					28:41	a Polysaccharide	26:41	a Polysaccharide from Moringa oleifera Leaf	26:68	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	5	44	theme	body	475:478	arg1	weight					480:485	the body weight	471:485	the body weight	471:485	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	0	45	theme	Moringa	48:54	arg1	Leaf					65:68	Moringa oleifera Leaf	48:68	Moringa oleifera Leaf	48:68	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	6	46	theme	immune	681:686	arg1	ability					713:719	the immune and intestinal digestive ability	677:719	the immune and intestinal digestive ability of the ICR mice	677:735	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	46	theme	immune	681:686	arg1	significant					742:752	significant	742:752	significant	742:752	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	1	47	theme	Moringa	99:105	arg1	plant					129:133	a natural plant	119:133	a natural plant with high nutritional and pharmacological value	119:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	1	47	theme	Moringa	99:105	arg1	oleifera					107:114	Moringa oleifera	99:114	Moringa oleifera	99:114	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	7	48	theme	chain	1055:1059	arg1	acid					1067:1070	chain fatty acid	1055:1070	short chain fatty acid production	1049:1081	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	6	49	theme	factor-alpha	840:851	arg1	index					776:780	the organ index	766:780	the organ index	766:780	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	49	theme	factor-alpha	840:851	arg1	enzymes					793:799	digestive enzymes	783:799	digestive enzymes	783:799	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	49	theme	factor-alpha	840:851	arg1	reduction					806:814	reduction	806:814	reduction of serum tumor necrosis factor-alpha and diamine oxidase levels	806:878	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	7	50	theme	microbial	919:927	arg1	composition					929:939	the microbial composition	915:939	the microbial composition of the intestinal tract in mice	915:971	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	6	51	theme	organ	770:774	arg1	index					776:780	the organ index	766:780	the organ index	766:780	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	52	theme	necrosis	831:838	arg1	factor-alpha					840:851	serum tumor necrosis factor-alpha	819:851	serum tumor necrosis factor-alpha	819:851	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	7	53	theme	intestinal	948:957	arg1	tract					959:963	the intestinal tract	944:963	the intestinal tract	944:963	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	6	54	theme	mice	732:735	arg1	ability					713:719	the immune and intestinal digestive ability	677:719	the immune and intestinal digestive ability of the ICR mice	677:735	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	6	54	theme	mice	732:735	arg1	significant					742:752	significant	742:752	significant	742:752	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	7	55	theme	tract	959:963	arg1	composition					929:939	the microbial composition	915:939	the microbial composition of the intestinal tract in mice	915:971	The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production.
31390269	2	56	theme	substances	234:243	arg1	variety					216:222	a variety	214:222	a variety of active substances	214:243	Leaves of M. oleifera contain a variety of active substances.
31390269	2	56	theme	substances	234:243	arg1	substances					234:243	active substances	227:243	active substances	227:243	Leaves of M. oleifera contain a variety of active substances.
31390269	2	57	contain	contain	206:212	arg1	Leaves					184:189	Leaves	184:189	Leaves	184:189	Leaves of M. oleifera contain a variety of active substances.
31390269	2	57	contain	contain	206:212	arg2	substances					234:243	active substances	227:243	active substances	227:243	Leaves of M. oleifera contain a variety of active substances.
31390269	2	57	contain	contain	206:212	arg2	variety					216:222	a variety	214:222	a variety of active substances	214:243	Leaves of M. oleifera contain a variety of active substances.
31390269	0	58	from	Microecology	77:88	arg1	Mice					93:96	Mice	93:96	Mice	93:96	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	2	59	theme	active	227:232	arg1	substances					234:243	active substances	227:243	active substances	227:243	Leaves of M. oleifera contain a variety of active substances.
31390269	1	60	theme	natural	121:127	arg1	plant					129:133	a natural plant	119:133	a natural plant with high nutritional and pharmacological value	119:181	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	1	60	theme	natural	121:127	arg1	oleifera					107:114	Moringa oleifera	99:114	Moringa oleifera	99:114	Moringa oleifera is a natural plant with high nutritional and pharmacological value.
31390269	5	61	theme	mice	590:593	arg1	digestion					509:517	intestinal digestion	498:517	intestinal digestion	498:517	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	5	61	theme	mice	590:593	arg1	weight					480:485	the body weight	471:485	the body weight	471:485	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	5	61	theme	mice	590:593	arg1	microenvironment					535:550	intestinal microenvironment	524:550	intestinal microenvironment	524:550	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	5	61	theme	mice	590:593	arg1	immunity					488:495	immunity	488:495	immunity	488:495	Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated.
31390269	0	62	theme	oleifera	56:63	arg1	Leaf					65:68	Moringa oleifera Leaf	48:68	Moringa oleifera Leaf	48:68	The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice.
31390269	6	63	theme	tumor	825:829	arg1	factor-alpha					840:851	serum tumor necrosis factor-alpha	819:851	serum tumor necrosis factor-alpha	819:851	After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels.
31390269	8	64	theme	health	1225:1230	arg1	effects					1242:1248	health promotion effects	1225:1248	health promotion effects	1225:1248	These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.
29580428	5	0	theme	breaking	1042:1049	arg1	strength					1051:1058	breaking strength	1042:1058	breaking strength	1042:1058	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	3	1	dep	types	525:529	arg1	CH3COONa					538:545	CH3COONa	538:545	CH3COONa	538:545	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	3	1	dep	types	525:529	arg1	NaCl					532:535	NaCl	532:535	NaCl	532:535	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	3	1	dep	types	525:529	arg1	Na2SO4					548:553	Na2SO4	548:553	Na2SO4	548:553	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	0	2	theme	sodium	66:71	arg1	interaction					51:61	the intermolecular interaction	32:61	the intermolecular interaction of sodium	32:71	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	5	3	theme	strength	1051:1058	arg1	content					987:993	the content	983:993	the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	983:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	4	theme	electron	823:830	arg1	microscope					832:841	scanning electron microscope	814:841	scanning electron microscope (SEM)	814:847	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	4	theme	electron	823:830	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy (FT-IR)	783:811	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	4	theme	electron	823:830	arg1	SEM					844:846	SEM	844:846	SEM	844:846	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	1	5	theme	antarctic	149:157	arg1	AKP					174:176	AKP	174:176	AKP	174:176	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	1	5	theme	antarctic	149:157	arg1	protein					165:171	antarctic krill protein	149:171	antarctic krill protein (AKP)	149:177	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	3	6	from	influences	499:508	arg1	interaction					578:588	the intermolecular interaction	559:588	the intermolecular interaction of SA/AKP composite fibers	559:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	0	7	theme	protein	98:104	arg1	fibers					116:121	krill protein composite fibers	92:121	krill protein composite fibers	92:121	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	0	8	from	Effects	0:6	arg1	interaction					51:61	the intermolecular interaction	32:61	the intermolecular interaction of sodium	32:71	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	5	9	theme	salt	951:954	arg1	types					935:939	the types	931:939	the types of sodium salt	931:954	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	10	theme	SA/AKP	724:729	arg1	fibers					741:746	SA/AKP composite fibers	724:746	SA/AKP composite fibers	724:746	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	3	11	theme	salt	520:523	arg1	types					525:529	sodium salt types	513:529	sodium salt types (NaCl, CH3COONa, Na2SO4)	513:554	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	5	12	theme	negligible	1122:1131	arg1	effect					1133:1138	a negligible effect	1120:1138	a negligible effect on the crystallinity and thermal stability	1120:1181	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	0	13	theme	krill	92:96	arg1	fibers					116:121	krill protein composite fibers	92:121	krill protein composite fibers	92:121	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	1	14	theme	conventional	240:251	arg1	method					266:271	the conventional wet spinning method	236:271	the conventional wet spinning method using 5% CaCl2 as coagulation solution	236:310	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	5	15	from	content	987:993	arg1	fibers					1103:1108	SA/AKP composite fibers	1086:1108	SA/AKP composite fibers	1086:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	16	theme	intermolecular	1012:1025	arg1	bond					1036:1039	intermolecular hydrogen bond	1012:1039	intermolecular hydrogen bond	1012:1039	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	17	theme	fibers	741:746	arg1	crystallinity					662:674	crystallinity	662:674	crystallinity	662:674	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	17	theme	fibers	741:746	arg1	stability					685:693	thermal stability	677:693	thermal stability	677:693	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	17	theme	fibers	741:746	arg1	properties					710:719	mechanical properties	699:719	mechanical properties	699:719	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	17	theme	fibers	741:746	arg1	morphology					650:659	morphology	650:659	morphology	650:659	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	17	theme	fibers	741:746	arg1	interaction					637:647	The intermolecular interaction	618:647	The intermolecular interaction	618:647	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	1	18	theme	krill	159:163	arg1	AKP					174:176	AKP	174:176	AKP	174:176	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	1	18	theme	krill	159:163	arg1	protein					165:171	antarctic krill protein	149:171	antarctic krill protein (AKP)	149:177	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	0	19	theme	composite	106:114	arg1	fibers					116:121	krill protein composite fibers	92:121	krill protein composite fibers	92:121	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	5	20	theme	hydrogen	1027:1034	arg1	bond					1036:1039	intermolecular hydrogen bond	1012:1039	intermolecular hydrogen bond	1012:1039	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	2	21	dep	degree	387:392	arg1	the					372:374	the	372:374	the	372:374	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	4	22	theme	composite	731:739	arg1	fibers					741:746	SA/AKP composite fibers	724:746	SA/AKP composite fibers	724:746	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	2	23	theme	SA/AKP	346:351	arg1	solution					353:360	the SA/AKP solution	342:360	the SA/AKP solution	342:360	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	5	24	theme	bond	1036:1039	arg1	content					987:993	the content	983:993	the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	983:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	25	theme	scanning	814:821	arg1	microscope					832:841	scanning electron microscope	814:841	scanning electron microscope (SEM)	814:847	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	25	theme	scanning	814:821	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy (FT-IR)	783:811	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	25	theme	scanning	814:821	arg1	SEM					844:846	SEM	844:846	SEM	844:846	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	5	26	theme	composite	1093:1101	arg1	fibers					1103:1108	SA/AKP composite fibers	1086:1108	SA/AKP composite fibers	1086:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	3	27	theme	types	525:529	arg1	influences					499:508	the influences	495:508	the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers	495:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	2	28	theme	intermolecular	398:411	arg1	interaction					413:423	intermolecular interaction	398:423	intermolecular interaction	398:423	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	1	29	theme	wet	253:255	arg1	method					266:271	the conventional wet spinning method	236:271	the conventional wet spinning method using 5% CaCl2 as coagulation solution	236:310	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	5	30	contain	have	1115:1118	arg1	types					935:939	the types	931:939	the types of sodium salt	931:954	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	30	contain	have	1115:1118	arg2	effect					1133:1138	a negligible effect	1120:1138	a negligible effect on the crystallinity and thermal stability	1120:1181	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	0	31	theme	salt	18:21	arg1	types					23:27	sodium salt types	11:27	sodium salt types	11:27	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	1	32	theme	spinning	257:264	arg1	method					266:271	the conventional wet spinning method	236:271	the conventional wet spinning method using 5% CaCl2 as coagulation solution	236:310	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	3	33	theme	sodium	513:518	arg1	types					525:529	sodium salt types	513:529	sodium salt types (NaCl, CH3COONa, Na2SO4)	513:554	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	2	34	theme	ionization	376:385	arg1	degree					387:392	ionization degree	376:392	ionization degree	376:392	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	0	35	theme	sodium	11:16	arg1	types					23:27	sodium salt types	11:27	sodium salt types	11:27	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	5	36	from	effect	1133:1138	arg1	crystallinity					1147:1159	crystallinity	1147:1159	crystallinity	1147:1159	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	36	from	effect	1133:1138	arg1	stability					1173:1181	thermal stability	1165:1181	thermal stability	1165:1181	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	37	dep	crystallinity	1147:1159	arg1	the					1143:1145	the	1143:1145	the	1143:1145	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	2	38	theme	sodium	317:322	arg1	salt					324:327	The sodium salt	313:327	The sodium salt	313:327	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	4	39	theme	thermogravimetric	875:891	arg1	TGA					903:905	TGA	903:905	TGA	903:905	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	39	theme	thermogravimetric	875:891	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy (FT-IR)	783:811	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	39	theme	thermogravimetric	875:891	arg1	analysis					893:900	thermogravimetric analysis	875:900	thermogravimetric analysis (TGA)	875:906	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	3	40	theme	SA/AKP	593:598	arg1	fibers					610:615	SA/AKP composite fibers	593:615	SA/AKP composite fibers	593:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	4	41	theme	infrared	783:790	arg1	microscope					832:841	scanning electron microscope	814:841	scanning electron microscope (SEM)	814:847	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	41	theme	infrared	783:790	arg1	FT-IR					806:810	FT-IR	806:810	FT-IR	806:810	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	41	theme	infrared	783:790	arg1	diffraction					856:866	x-ray diffraction	850:866	x-ray diffraction (XRD)	850:872	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	41	theme	infrared	783:790	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy (FT-IR)	783:811	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	41	theme	infrared	783:790	arg1	analysis					893:900	thermogravimetric analysis	875:900	thermogravimetric analysis (TGA)	875:906	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	5	42	from	influences	969:978	arg1	content					987:993	the content	983:993	the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	983:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	0	43	theme	types	23:27	arg1	Effects					0:6	Effects	0:6	Effects of sodium salt types on the intermolecular interaction of sodium	0:71	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	1	44	theme	5	279:279	arg1	%					280:280	%	280:280	%	280:280	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	2	45	theme	system	438:443	arg1	degree					387:392	ionization degree	376:392	ionization degree	376:392	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	2	45	theme	system	438:443	arg1	interaction					413:423	intermolecular interaction	398:423	intermolecular interaction	398:423	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	3	46	theme	intermolecular	563:576	arg1	interaction					578:588	the intermolecular interaction	559:588	the intermolecular interaction of SA/AKP composite fibers	559:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	5	47	theme	sodium	944:949	arg1	salt					951:954	sodium salt	944:954	sodium salt	944:954	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	0	48	theme	intermolecular	36:49	arg1	interaction					51:61	the intermolecular interaction	32:61	the intermolecular interaction of sodium	32:71	Effects of sodium salt types on the intermolecular interaction of sodium alginate/antarctic krill protein composite fibers.
29580428	1	49	theme	%	280:280	arg1	CaCl2					282:286	5% CaCl2	279:286	5% CaCl2	279:286	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	5	50	theme	β-sheet	1003:1009	arg1	content					987:993	the content	983:993	the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	983:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	51	theme	surface	1064:1070	arg1	morphology					1072:1081	surface morphology	1064:1081	surface morphology	1064:1081	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	52	dep	fourier	765:771	arg1	transform					773:781	transform	773:781	transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA)	773:906	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	5	53	theme	obvious	961:967	arg1	influences					969:978	obvious influences	961:978	obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	961:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	3	54	theme	study	471:475	arg1	purpose					455:461	The main purpose	446:461	The main purpose of this study	446:475	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	1	55	theme	Sodium	124:129	arg1	alginate					131:138	Sodium alginate	124:138	Sodium alginate (SA)	124:143	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	1	55	theme	Sodium	124:129	arg1	SA					141:142	SA	141:142	SA	141:142	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	5	56	contain	have	956:959	arg1	types					935:939	the types	931:939	the types of sodium salt	931:954	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	56	contain	have	956:959	arg2	influences					969:978	obvious influences	961:978	obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	961:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	5	57	theme	SA/AKP	1086:1091	arg1	fibers					1103:1108	SA/AKP composite fibers	1086:1108	SA/AKP composite fibers	1086:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	4	58	theme	thermal	677:683	arg1	stability					685:693	thermal stability	677:693	thermal stability	677:693	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	59	theme	x-ray	850:854	arg1	XRD					869:871	XRD	869:871	XRD	869:871	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	59	theme	x-ray	850:854	arg1	diffraction					856:866	x-ray diffraction	850:866	x-ray diffraction (XRD)	850:872	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	4	59	theme	x-ray	850:854	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy (FT-IR)	783:811	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	3	60	theme	composite	600:608	arg1	fibers					610:615	SA/AKP composite fibers	593:615	SA/AKP composite fibers	593:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	4	61	theme	mechanical	699:708	arg1	properties					710:719	mechanical properties	699:719	mechanical properties	699:719	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	3	62	theme	main	450:453	arg1	purpose					455:461	The main purpose	446:461	The main purpose of this study	446:475	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	5	63	theme	morphology	1072:1081	arg1	content					987:993	the content	983:993	the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers	983:1108	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	3	64	theme	fibers	610:615	arg1	interaction					578:588	the intermolecular interaction	559:588	the intermolecular interaction of SA/AKP composite fibers	559:615	The main purpose of this study is to investigate the influences of sodium salt types (NaCl, CH3COONa, Na2SO4) on the intermolecular interaction of SA/AKP composite fibers.
29580428	1	65	theme	SA/AKP	209:214	arg1	fibers					226:231	the SA/AKP composite fibers	205:231	the SA/AKP composite fibers	205:231	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	2	66	theme	composite	428:436	arg1	system					438:443	composite system	428:443	composite system	428:443	The sodium salt was added to the SA/AKP solution to adjust the ionization degree and intermolecular interaction of composite system.
29580428	1	67	theme	coagulation	291:301	arg1	solution					303:310	coagulation solution	291:310	coagulation solution	291:310	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
29580428	4	68	theme	intermolecular	622:635	arg1	interaction					637:647	The intermolecular interaction	618:647	The intermolecular interaction	618:647	The intermolecular interaction, morphology, crystallinity, thermal stability and mechanical properties of SA/AKP composite fibers were analyzed by fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), x-ray diffraction (XRD), thermogravimetric analysis (TGA).
29580428	5	69	theme	thermal	1165:1171	arg1	stability					1173:1181	thermal stability	1165:1181	thermal stability	1165:1181	The results show that the types of sodium salt have obvious influences on the content of both β-sheet, intermolecular hydrogen bond, breaking strength and surface morphology in SA/AKP composite fibers, but have a negligible effect on the crystallinity and thermal stability.
29580428	1	70	theme	composite	216:224	arg1	fibers					226:231	the SA/AKP composite fibers	205:231	the SA/AKP composite fibers	205:231	Sodium alginate (SA) and antarctic krill protein (AKP) were blended to fabricate the SA/AKP composite fibers by the conventional wet spinning method using 5% CaCl2 as coagulation solution.
31730995	4	0	theme	enriched	647:654	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	1	theme	tissue-derived	807:820	arg1	cells					827:831	human adipose tissue-derived stem cells	793:831	human adipose tissue-derived stem cells	793:831	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	2	theme	hydrogels	659:667	arg1	behavior					596:603	the mineralization behavior	577:603	the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels	577:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	3	theme	adipose	799:805	arg1	cells					827:831	human adipose tissue-derived stem cells	793:831	human adipose tissue-derived stem cells	793:831	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	4	theme	3D	656:657	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	8	5	theme	gellan	1423:1428	arg1	biomaterials					1440:1451	gellan gum-based biomaterials	1423:1451	gellan gum-based biomaterials	1423:1451	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	6	6	theme	Alizarin	1176:1183	arg1	staining					1191:1198	Alizarin Red S staining	1176:1198	Alizarin Red S staining	1176:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	5	7	theme	acellular	901:909	arg1	hydrogels					923:931	acellular spongy-like hydrogels	901:931	acellular spongy-like hydrogels	901:931	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	4	8	theme	fibroin	613:619	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	8	9	theme	alternative	1343:1353	arg1	strategy					1355:1362	cheap alternative strategy	1337:1362	cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials	1337:1451	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	4	10	theme	stem	822:825	arg1	cells					827:831	human adipose tissue-derived stem cells	793:831	human adipose tissue-derived stem cells	793:831	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	2	11	theme	organic	248:254	arg1	template					256:263	an organic template	245:263	an organic template like fibrillar Collagen I under physiological condition	245:319	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	5	12	theme	fibroin	863:869	arg1	Incorporation					841:853	Incorporation	841:853	Incorporation of silk fibroin	841:869	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	3	13	theme	Fibrous	322:328	arg1	fibroin					335:341	Fibrous silk fibroin	322:341	Fibrous silk fibroin	322:341	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	14	theme	simulated	450:458	arg1	SBF					472:474	SBF	472:474	SBF	472:474	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	14	theme	simulated	450:458	arg1	fluid					465:469	simulated body fluid	450:469	simulated body fluid (SBF)	450:475	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	5	15	theme	silk	858:861	arg1	fibroin					863:869	silk fibroin	858:869	silk fibroin	858:869	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	3	16	theme	HAp	414:416	arg1	mineralization					418:431	HAp mineralization	414:431	HAp mineralization	414:431	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	17	theme	silk	330:333	arg1	fibroin					335:341	Fibrous silk fibroin	322:341	Fibrous silk fibroin	322:341	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	18	dep	acts	386:389	arg1	considered					517:526	considered	517:526	considered as popular bone regeneration biomaterial	517:567	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	8	19	theme	fibroin	1322:1328	arg1	blending					1310:1317	The blending	1306:1317	The blending of fibroin	1306:1328	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	4	20	theme	silk	608:611	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	21	theme	human	793:797	arg1	cells					827:831	human adipose tissue-derived stem cells	793:831	human adipose tissue-derived stem cells	793:831	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	1	22	theme	natural	82:88	arg1	process					90:96	a natural process	80:96	a natural process leading to the formation of mineralized tissue such as bone	80:156	Mineralization is a natural process leading to the formation of mineralized tissue such as bone.
31730995	1	22	theme	natural	82:88	arg1	Mineralization					62:75	Mineralization	62:75	Mineralization	62:75	Mineralization is a natural process leading to the formation of mineralized tissue such as bone.
31730995	4	23	theme	gellan	636:641	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	0	24	theme	Silk	0:3	arg1	fibroin					5:11	Silk fibroin	0:11	Silk fibroin	0:11	Silk fibroin promotes mineralization of gellan gum hydrogels.
31730995	4	25	theme	closer	702:707	arg1	conditions					691:700	conditions	691:700	conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs)	691:838	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	26	theme	self-assembled	621:634	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	4	27	link	tissue-derived	807:820	arg1	cells					827:831	human adipose tissue-derived stem cells	793:831	human adipose tissue-derived stem cells	793:831	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	6	28	theme	S	1189:1189	arg1	staining					1191:1198	Alizarin Red S staining	1176:1198	Alizarin Red S staining	1176:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	2	29	theme	fibrillar	270:278	arg1	Collagen					280:287	fibrillar Collagen I	270:289	fibrillar Collagen I under physiological condition	270:319	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	4	30	theme	mineralization	581:594	arg1	behavior					596:603	the mineralization behavior	577:603	the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels	577:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	8	31	theme	cheap	1337:1341	arg1	strategy					1355:1362	cheap alternative strategy	1337:1362	cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials	1337:1451	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	6	32	theme	expression	1153:1162	arg1	profile					1164:1170	gene expression profile	1148:1170	gene expression profile	1148:1170	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	2	33	theme	mineral	169:175	arg1	hydroxyapatite					198:211	hydroxyapatite	198:211	hydroxyapatite (HAp)	198:217	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	2	33	theme	mineral	169:175	arg1	component					177:185	The chief mineral component	159:185	The chief mineral component of bone	159:193	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	4	34	theme	physiological	712:724	arg1	ones					726:729	physiological ones	712:729	physiological ones using SBF as well as FBS	712:754	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	3	35	theme	fetal	480:484	arg1	FBS					500:502	FBS	500:502	FBS	500:502	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	35	theme	fetal	480:484	arg1	serum					493:497	fetal bovine serum	480:497	fetal bovine serum (FBS)	480:503	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	0	36	theme	gellan	40:45	arg1	hydrogels					51:59	gellan gum hydrogels	40:59	gellan gum hydrogels	40:59	Silk fibroin promotes mineralization of gellan gum hydrogels.
31730995	4	37	dep	cells	827:831	arg1	e.g.					788:791	e.g.	788:791	e.g.	788:791	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	2	38	theme	chief	163:167	arg1	hydroxyapatite					198:211	hydroxyapatite	198:211	hydroxyapatite (HAp)	198:217	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	2	38	theme	chief	163:167	arg1	component					177:185	The chief mineral component	159:185	The chief mineral component of bone	159:193	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	3	39	theme	bone	539:542	arg1	biomaterial					557:567	popular bone regeneration biomaterial	531:567	popular bone regeneration biomaterial	531:567	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	40	theme	nucleation	394:403	arg1	site					405:408	nucleation site	394:408	nucleation site for HAp mineralization	394:431	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	6	41	located	found	1042:1046	arg2	mineralization					1024:1037	mineralization	1024:1037	mineralization	1024:1037	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	6	41	located	found	1042:1046	arg1	compositions					1064:1075	all hydrogel compositions	1051:1075	all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining	1051:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	3	42	theme	regeneration	544:555	arg1	biomaterial					557:567	popular bone regeneration biomaterial	531:567	popular bone regeneration biomaterial	531:567	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	0	43	theme	hydrogels	51:59	arg1	mineralization					22:35	mineralization	22:35	mineralization of gellan gum hydrogels	22:59	Silk fibroin promotes mineralization of gellan gum hydrogels.
31730995	8	44	theme	gum-based	1430:1438	arg1	biomaterials					1440:1451	gellan gum-based biomaterials	1423:1451	gellan gum-based biomaterials	1423:1451	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	5	45	theme	SEM-EDS	979:985	arg1	analysis					987:994	SEM-EDS analysis	979:994	SEM-EDS analysis	979:994	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	6	46	theme	hydrogel	1055:1062	arg1	compositions					1064:1075	all hydrogel compositions	1051:1075	all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining	1051:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	6	47	theme	gene	1148:1151	arg1	profile					1164:1170	gene expression profile	1148:1170	gene expression profile	1148:1170	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	0	48	theme	gum	47:49	arg1	hydrogels					51:59	gellan gum hydrogels	40:59	gellan gum hydrogels	40:59	Silk fibroin promotes mineralization of gellan gum hydrogels.
31730995	4	49	theme	cells	781:785	arg1	presence					769:776	presence	769:776	presence of cells (e.g. human adipose tissue-derived stem cells, ASCs)	769:838	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	3	50	theme	popular	531:537	arg1	biomaterial					557:567	popular bone regeneration biomaterial	531:567	popular bone regeneration biomaterial	531:567	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	6	51	theme	3 weeks	1080:1086	arg1	compositions					1064:1075	all hydrogel compositions	1051:1075	all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining	1051:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	5	52	from	mineralization	883:896	arg1	hydrogels					923:931	acellular spongy-like hydrogels	901:931	acellular spongy-like hydrogels	901:931	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	8	53	theme	mineralized	1398:1408	arg1	tissue					1410:1415	mineralized tissue	1398:1415	mineralized tissue	1398:1415	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	2	54	theme	bone	190:193	arg1	hydroxyapatite					198:211	hydroxyapatite	198:211	hydroxyapatite (HAp)	198:217	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	2	54	theme	bone	190:193	arg1	component					177:185	The chief mineral component	159:185	The chief mineral component of bone	159:193	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	6	55	theme	Red	1185:1187	arg1	staining					1191:1198	Alizarin Red S staining	1176:1198	Alizarin Red S staining	1176:1198	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	4	56	theme	gum	643:645	arg1	hydrogels					659:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	silk fibroin self-assembled gellan gum enriched 3D hydrogels	608:667	Hence, the mineralization behavior of silk fibroin self-assembled gellan gum enriched 3D hydrogels is investigated under conditions closer to physiological ones using SBF as well as FBS, and also in presence of cells (e.g. human adipose tissue-derived stem cells, ASCs).
31730995	2	57	theme	physiological	297:309	arg1	condition					311:319	physiological condition	297:319	physiological condition	297:319	The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition.
31730995	6	58	theme	osteogenic	1107:1116	arg1	conditions					1118:1127	osteogenic conditions	1107:1127	osteogenic conditions	1107:1127	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	5	59	theme	dependent	948:956	arg1	manner					958:963	composition dependent manner	936:963	composition dependent manner	936:963	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	1	60	theme	mineralized	126:136	arg1	tissue					138:143	mineralized tissue	126:143	mineralized tissue	126:143	Mineralization is a natural process leading to the formation of mineralized tissue such as bone.
31730995	7	61	theme	cell-mediated	1276:1288	arg1	mineralization					1290:1303	cell-mediated mineralization	1276:1303	cell-mediated mineralization	1276:1303	This is perhaps due to the co-existence of fibroin and FBS together induce cell-mediated mineralization.
31730995	3	62	theme	body	460:463	arg1	SBF					472:474	SBF	472:474	SBF	472:474	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	62	theme	body	460:463	arg1	fluid					465:469	simulated body fluid	450:469	simulated body fluid (SBF)	450:475	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	8	63	theme	tissue	1410:1415	arg1	repair					1388:1393	the repair	1384:1393	the repair of mineralized tissue using gellan gum-based biomaterials	1384:1451	The blending of fibroin offers cheap alternative strategy to improve or guide the repair of mineralized tissue using gellan gum-based biomaterials.
31730995	1	64	theme	tissue	138:143	arg1	formation					113:121	the formation	109:121	the formation of mineralized tissue such as bone	109:156	Mineralization is a natural process leading to the formation of mineralized tissue such as bone.
31730995	5	65	theme	spongy-like	911:921	arg1	hydrogels					923:931	acellular spongy-like hydrogels	901:931	acellular spongy-like hydrogels	901:931	Incorporation of silk fibroin induces the mineralization in acellular spongy-like hydrogels in composition dependent manner, confirmed by SEM-EDS analysis.
31730995	6	66	dep	3 weeks	1080:1086	arg1	post-culture					1088:1099	post-culture	1088:1099	post-culture	1088:1099	In contrast, ASCs mediated mineralization is found in all hydrogel compositions of 3 weeks post-culture under osteogenic conditions as demonstrated by gene expression profile and Alizarin Red S staining.
31730995	3	67	theme	bovine	486:491	arg1	FBS					500:502	FBS	500:502	FBS	500:502	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
31730995	3	67	theme	bovine	486:491	arg1	serum					493:497	fetal bovine serum	480:497	fetal bovine serum (FBS)	480:503	Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site for HAp mineralization when immersed in simulated body fluid (SBF) or fetal bovine serum (FBS), therefore, considered as popular bone regeneration biomaterial.
30472858	9	0	theme	products	1362:1369	arg1	products					1362:1369	the most innovative products	1342:1369	the most innovative products	1342:1369	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	9	0	theme	products	1362:1369	arg1	one					1335:1337	one	1335:1337	one	1335:1337	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	6	1	dep	98	1093:1094	arg1	to					1090:1091	to	1090:1091	to	1090:1091	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	5	2	theme	gas	899:901	arg1	treatment					922:930	an oxygen/helium gas atmospheric plasma treatment	882:930	an oxygen/helium gas atmospheric plasma treatment	882:930	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	9	3	theme	bactericidal	1291:1302	arg1	tissue					1304:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	5	4	theme	atmospheric	903:913	arg1	treatment					922:930	an oxygen/helium gas atmospheric plasma treatment	882:930	an oxygen/helium gas atmospheric plasma treatment	882:930	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	9	5	theme	societal	1498:1505	arg1	benefits					1507:1514	significant societal benefits	1486:1514	significant societal benefits	1486:1514	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	1	6	theme	chitosan	288:295	arg1	spray-coating					271:283	a hydrophobic spray-coating	257:283	a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite	257:344	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	5	7	theme	antimicrobial	968:980	arg1	papers					989:994	the best performing antimicrobial tissue papers	948:994	the best performing antimicrobial tissue papers	948:994	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	5	8	theme	performing	957:966	arg1	papers					989:994	the best performing antimicrobial tissue papers	948:994	the best performing antimicrobial tissue papers	948:994	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	3	9	dep	Escherichia	684:694	arg1	coli					696:699	coli	696:699	coli	696:699	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	3	10	theme	human	731:735	arg1	hand					737:740	a human hand	729:740	a human hand	729:740	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	5	11	theme	tissue	982:987	arg1	papers					989:994	the best performing antimicrobial tissue papers	948:994	the best performing antimicrobial tissue papers	948:994	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	1	12	theme	heightened	85:94	arg1	need					96:99	A heightened need to control the spread of infectious diseases	83:144	A heightened need to control the spread of infectious diseases	83:144	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	5	13	theme	cationized	857:866	arg1	paper					875:879	cationized tissue paper	857:879	cationized tissue paper	857:879	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	8	14	theme	absorption	1199:1208	arg1	properties					1210:1219	Water absorption properties	1193:1219	Water absorption properties	1193:1219	Water absorption properties were reduced with Ch but increased with CNC.
30472858	3	15	theme	Light-weight	505:516	arg1	handsheets					525:534	Light-weight tissue handsheets	505:534	Light-weight tissue handsheets	505:534	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	2	16	theme	chitosan	398:405	arg1	nature					388:393	the hydrophobic nature	372:393	the hydrophobic nature of chitosan	372:405	It was hypothesized that the hydrophobic nature of chitosan could be counterbalanced by the addition of CNC to maintain fiber formation and water absorbency.
30472858	1	17	theme	functionalized	181:194	arg1	paper					232:236	functionalized and innovative antimicrobial tissue paper	181:236	functionalized and innovative antimicrobial tissue paper	181:236	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	8	18	theme	Water	1193:1197	arg1	properties					1210:1219	Water absorption properties	1193:1219	Water absorption properties	1193:1219	Water absorption properties were reduced with Ch but increased with CNC.
30472858	0	19	theme	Tissue	69:74	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	4	20	theme	Water	769:773	arg1	absorption					775:784	Water absorption	769:784	Water absorption	769:784	Water absorption and strength properties were also analyzed.
30472858	6	21	theme	microbial	1097:1105	arg1	growth					1107:1112	up to 98% microbial growth	1087:1112	up to 98% microbial growth	1087:1112	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	5	22	theme	tissue	868:873	arg1	paper					875:879	cationized tissue paper	857:879	cationized tissue paper	857:879	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	0	23	theme	Antibacterial	14:26	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	9	24	contain	has	1311:1313	arg2	potential					1319:1327	the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits	1315:1514	the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits	1315:1514	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	9	24	contain	has	1311:1313	arg1	tissue					1304:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	1	25	theme	innovative	200:209	arg1	paper					232:236	functionalized and innovative antimicrobial tissue paper	181:236	functionalized and innovative antimicrobial tissue paper	181:236	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	0	26	theme	High-Strength	0:12	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	9	27	theme	most	1346:1349	arg1	products					1362:1369	the most innovative products	1342:1369	the most innovative products	1342:1369	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	9	28	theme	self-disinfecting	1272:1288	arg1	tissue					1304:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	1	29	theme	antimicrobial	211:223	arg1	paper					232:236	functionalized and innovative antimicrobial tissue paper	181:236	functionalized and innovative antimicrobial tissue paper	181:236	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	2	30	theme	CNC	451:453	arg1	addition					439:446	the addition	435:446	the addition of CNC to maintain fiber formation and water absorbency	435:502	It was hypothesized that the hydrophobic nature of chitosan could be counterbalanced by the addition of CNC to maintain fiber formation and water absorbency.
30472858	3	31	theme	rest	758:761	arg1	room					763:766	the rest room	754:766	the rest room	754:766	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	5	32	theme	plasma	915:920	arg1	treatment					922:930	an oxygen/helium gas atmospheric plasma treatment	882:930	an oxygen/helium gas atmospheric plasma treatment	882:930	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	6	33	dep	%	1095:1095	arg1	98					1093:1094	98	1093:1094	98	1093:1094	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	1	34	theme	tissue	225:230	arg1	paper					232:236	functionalized and innovative antimicrobial tissue paper	181:236	functionalized and innovative antimicrobial tissue paper	181:236	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	0	35	theme	Chitosan-Cellulose	28:45	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	1	36	theme	cellulose	306:314	arg1	CNCs					330:333	CNCs	330:333	CNCs	330:333	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	1	36	theme	cellulose	306:314	arg1	nanocrystals					316:327	cellulose nanocrystals	306:327	cellulose nanocrystals (CNCs)	306:334	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	3	37	theme	tissue	518:523	arg1	handsheets					525:534	Light-weight tissue handsheets	505:534	Light-weight tissue handsheets	505:534	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	2	38	theme	water	487:491	arg1	absorbency					493:502	water absorbency	487:502	water absorbency	487:502	It was hypothesized that the hydrophobic nature of chitosan could be counterbalanced by the addition of CNC to maintain fiber formation and water absorbency.
30472858	1	39	theme	nanocrystals	316:327	arg1	spray-coating					271:283	a hydrophobic spray-coating	257:283	a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite	257:344	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	6	40	dep	growth	1107:1112	arg1	%					1095:1095	%	1095:1095	%	1095:1095	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	9	41	theme	surfaces	1451:1458	arg1	infection					1438:1446	the infection	1434:1446	the infection of surfaces	1434:1458	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	9	42	theme	innovative	1351:1360	arg1	products					1362:1369	the most innovative products	1342:1369	the most innovative products	1342:1369	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	0	43	theme	Composite	59:67	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	5	44	theme	paper	875:879	arg1	surface					846:852	the surface	842:852	the surface of cationized tissue paper	842:879	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	6	45	theme	bactericidal	1009:1020	arg1	activity					1022:1029	The highest bactericidal activity	997:1029	The highest bactericidal activity	997:1029	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	0	46	theme	Nanocrystal	47:57	arg1	Paper					76:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper	0:80	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.	0:81	High-Strength Antibacterial Chitosan-Cellulose Nanocrystal Composite Tissue Paper.
30472858	6	47	theme	tissue	1062:1067	arg1	paper					1069:1073	ChCNC-coated tissue paper	1049:1073	ChCNC-coated tissue paper	1049:1073	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	7	48	theme	antimicrobial	1153:1165	arg1	activity					1167:1174	the antimicrobial activity	1149:1174	the antimicrobial activity of the coatings	1149:1190	Plasma treatment further improved the antimicrobial activity of the coatings.
30472858	9	49	theme	hygiene	1379:1385	arg1	industry					1387:1394	the hygiene industry	1375:1394	the hygiene industry	1375:1394	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	3	50	theme	antimicrobial	630:642	arg1	activity					644:651	antimicrobial activity	630:651	antimicrobial activity against Gram-negative bacteria Escherichia coli	630:699	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	3	51	theme	composite	588:596	arg1	ChCNC					607:611	ChCNC	607:611	ChCNC	607:611	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	3	51	theme	composite	588:596	arg1	coating					598:604	their composite coating	582:604	their composite coating (ChCNC)	582:612	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	1	52	theme	infectious	126:135	arg1	diseases					137:144	infectious diseases	126:144	infectious diseases	126:144	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	2	53	theme	fiber	467:471	arg1	formation					473:481	fiber formation	467:481	fiber formation	467:481	It was hypothesized that the hydrophobic nature of chitosan could be counterbalanced by the addition of CNC to maintain fiber formation and water absorbency.
30472858	3	54	theme	Escherichia	684:694	arg1	bacteria					675:682	Gram-negative bacteria	661:682	Gram-negative bacteria Escherichia coli	661:699	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	7	55	theme	Plasma	1115:1120	arg1	treatment					1122:1130	Plasma treatment	1115:1130	Plasma treatment	1115:1130	Plasma treatment further improved the antimicrobial activity of the coatings.
30472858	1	56	theme	diseases	137:144	arg1	spread					116:121	the spread	112:121	the spread of infectious diseases	112:144	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	7	57	theme	coatings	1183:1190	arg1	activity					1167:1174	the antimicrobial activity	1149:1174	the antimicrobial activity of the coatings	1149:1190	Plasma treatment further improved the antimicrobial activity of the coatings.
30472858	9	58	theme	"	1289:1289	arg1	tissue					1304:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue	1266:1309	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	4	59	theme	strength	790:797	arg1	properties					799:808	strength properties	790:808	strength properties	790:808	Water absorption and strength properties were also analyzed.
30472858	6	60	theme	ChCNC-coated	1049:1060	arg1	paper					1069:1073	ChCNC-coated tissue paper	1049:1073	ChCNC-coated tissue paper	1049:1073	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	3	61	theme	Gram-negative	661:673	arg1	bacteria					675:682	Gram-negative bacteria	661:682	Gram-negative bacteria Escherichia coli	661:699	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	5	62	theme	oxygen/helium	885:897	arg1	treatment					922:930	an oxygen/helium gas atmospheric plasma treatment	882:930	an oxygen/helium gas atmospheric plasma treatment	882:930	To activate the surface of cationized tissue paper, an oxygen/helium gas atmospheric plasma treatment was employed on the best performing antimicrobial tissue papers.
30472858	9	63	theme	significant	1486:1496	arg1	benefits					1507:1514	significant societal benefits	1486:1514	significant societal benefits	1486:1514	This "self-disinfecting" bactericidal tissue has the potential to be one of the most innovative products for the hygiene industry because it can dry, clean, and resist the infection of surfaces simultaneously, providing significant societal benefits.
30472858	1	64	dep	chitosan	288:295	arg1	composite					336:344	composite	336:344	composite	336:344	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	1	65	theme	current	159:165	arg1	work					167:170	the current work	155:170	the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite	155:344	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	1	66	theme	hydrophobic	259:269	arg1	spray-coating					271:283	a hydrophobic spray-coating	257:283	a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite	257:344	A heightened need to control the spread of infectious diseases prompted the current work in which functionalized and innovative antimicrobial tissue paper was developed with a hydrophobic spray-coating of chitosan (Ch) and cellulose nanocrystals (CNCs) composite.
30472858	6	67	theme	highest	1001:1007	arg1	activity					1022:1029	The highest bactericidal activity	997:1029	The highest bactericidal activity	997:1029	The highest bactericidal activity was observed with ChCNC-coated tissue paper, inhibiting up to 98% microbial growth.
30472858	3	68	theme	microbial	707:715	arg1	sample					717:722	a microbial sample	705:722	a microbial sample from a human hand	705:740	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	3	69	from	hand	737:740	arg1	activity					644:651	antimicrobial activity	630:651	antimicrobial activity against Gram-negative bacteria Escherichia coli	630:699	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	3	69	from	hand	737:740	arg1	sample					717:722	a microbial sample	705:722	a microbial sample from a human hand	705:740	Light-weight tissue handsheets were prepared, spray-coated with Ch, CNC, and their composite coating (ChCNC), and tested for antimicrobial activity against Gram-negative bacteria Escherichia coli and a microbial sample from a human hand after using the rest room.
30472858	2	70	theme	hydrophobic	376:386	arg1	nature					388:393	the hydrophobic nature	372:393	the hydrophobic nature of chitosan	372:405	It was hypothesized that the hydrophobic nature of chitosan could be counterbalanced by the addition of CNC to maintain fiber formation and water absorbency.
31261807	8	0	theme	natural	1060:1066	arg1	NK					1076:1077	NK	1076:1077	NK	1076:1077	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	8	0	theme	natural	1060:1066	arg1	killer					1068:1073	natural killer	1060:1073	natural killer (NK) cells	1060:1084	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	6	1	theme	inducible	853:861	arg1	iNOS					886:889	iNOS	886:889	iNOS	886:889	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	1	theme	inducible	853:861	arg1	synthase					876:883	inducible nitric oxide synthase	853:883	inducible nitric oxide synthase (iNOS)	853:890	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	2	theme	factor	911:916	arg1	production					815:824	nitric oxide (NO) production	797:824	nitric oxide (NO) production	797:824	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	2	theme	factor	911:916	arg1	expression					839:848	the mRNA expression	830:848	the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α	830:924	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	7	3	theme	non-toxic	947:955	arg1	grade					957:961	the non-toxic grade	943:961	the non-toxic grade in mice	943:969	GPP belonged to the non-toxic grade in mice.
31261807	5	4	theme	GPP	612:614	arg1	toxicity					600:607	the acute oral toxicity	585:607	the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice	585:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	9	5	theme	PUP	1366:1368	arg1	utilization					1343:1353	utilization	1343:1353	utilization	1343:1353	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	9	5	theme	PUP	1366:1368	arg1	development					1327:1337	further development	1319:1337	further development	1319:1337	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	9	6	from	GLP	1358:1360	arg1	function					1380:1387	immune function	1373:1387	immune function	1373:1387	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	0	7	theme	Innate	107:112	arg1	Function					121:128	Innate Immune Function	107:128	Innate Immune Function in Mice	107:136	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	4	8	located	detected	545:552	arg2	immunoregulation					517:532	The immunoregulation	513:532	The immunoregulation of GPP	513:539	The immunoregulation of GPP was detected in RAW264.7 macrophages.
31261807	4	8	located	detected	545:552	arg1	macrophages					566:576	RAW264.7 macrophages	557:576	RAW264.7 macrophages	557:576	The immunoregulation of GPP was detected in RAW264.7 macrophages.
31261807	9	9	from	PUP	1366:1368	arg1	function					1380:1387	immune function	1373:1387	immune function	1373:1387	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	4	10	theme	RAW264.7	557:564	arg1	macrophages					566:576	RAW264.7 macrophages	557:576	RAW264.7 macrophages	557:576	The immunoregulation of GPP was detected in RAW264.7 macrophages.
31261807	6	11	theme	tumor	896:900	arg1	TNF					919:921	TNF	919:921	TNF	919:921	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	11	theme	tumor	896:900	arg1	factor					911:916	tumor necrosis factor	896:916	tumor necrosis factor (TNF)	896:922	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	12	theme	mRNA	834:837	arg1	expression					839:848	the mRNA expression	830:848	the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α	830:924	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	13	theme	cells	753:757	arg1	function					721:728	the function	717:728	the function of macrophage RAW264.7 cells	717:757	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	9	14	theme	immune	1373:1378	arg1	function					1380:1387	immune function	1373:1387	immune function	1373:1387	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	1	15	theme	Modern	139:144	arg1	research					146:153	Modern research	139:153	Modern research	139:153	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	9	16	theme	further	1319:1325	arg1	development					1327:1337	further development	1319:1337	further development	1319:1337	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	6	17	theme	RAW264.7	744:751	arg1	cells					753:757	macrophage RAW264.7 cells	733:757	macrophage RAW264.7 cells	733:757	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	9	18	theme	immune	1269:1274	arg1	function					1276:1283	immune function	1269:1283	immune function	1269:1283	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	1	19	theme	Ganoderma	170:178	arg1	lucidum					180:186	Ganoderma lucidum	170:186	Ganoderma lucidum polysaccharides (GLP)	170:208	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	0	20	theme	Immune	114:119	arg1	Function					121:128	Innate Immune Function	107:128	Innate Immune Function in Mice	107:136	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	5	21	theme	acute	589:593	arg1	toxicity					600:607	the acute oral toxicity	585:607	the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice	585:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	6	22	theme	macrophage	733:742	arg1	cells					753:757	macrophage RAW264.7 cells	733:757	macrophage RAW264.7 cells	733:757	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	9	23	dep	development	1327:1337	arg1	the					1315:1317	the	1315:1317	the	1315:1317	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	1	24	theme	lucidum	180:186	arg1	polysaccharides					188:202	Ganoderma lucidum polysaccharides	170:202	Ganoderma lucidum polysaccharides (GLP)	170:208	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	1	24	theme	lucidum	180:186	arg1	GLP					205:207	GLP	205:207	GLP	205:207	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	5	25	theme	oral	595:598	arg1	toxicity					600:607	the acute oral toxicity	585:607	the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice	585:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	9	26	from	utilization	1343:1353	arg1	function					1380:1387	immune function	1373:1387	immune function	1373:1387	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	8	27	theme	cells	1080:1084	arg1	function					1031:1038	macrophage phagocytic function	1009:1038	macrophage phagocytic function	1009:1038	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	8	27	theme	cells	1080:1084	arg1	activity					1048:1055	the activity	1044:1055	the activity of natural killer (NK) cells	1044:1084	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	6	28	theme	necrosis	902:909	arg1	TNF					919:921	TNF	919:921	TNF	919:921	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	28	theme	necrosis	902:909	arg1	factor					911:916	tumor necrosis factor	896:916	tumor necrosis factor (TNF)	896:922	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	29	theme	oxide	870:874	arg1	iNOS					886:889	iNOS	886:889	iNOS	886:889	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	29	theme	oxide	870:874	arg1	synthase					876:883	inducible nitric oxide synthase	853:883	inducible nitric oxide synthase (iNOS)	853:890	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	30	theme	nitric	797:802	arg1	NO					811:812	NO	811:812	NO	811:812	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	30	theme	nitric	797:802	arg1	oxide					804:808	nitric oxide	797:808	nitric oxide (NO) production	797:824	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	5	31	from	toxicity	600:607	arg1	mice					661:664	mice	661:664	mice	661:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	5	31	from	toxicity	600:607	arg1	immunoregulation					641:656	immunoregulation	641:656	immunoregulation	641:656	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	8	32	theme	body	1155:1158	arg1	weight					1160:1165	0, 3.6, 120, 360 mg/kg body weight	1132:1165	0, 3.6, 120, 360 mg/kg body weight (mg/kg BW)	1132:1176	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	8	32	theme	body	1155:1158	arg1	BW					1174:1175	mg/kg BW	1168:1175	mg/kg BW	1168:1175	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	9	33	from	development	1327:1337	arg1	function					1380:1387	immune function	1373:1387	immune function	1373:1387	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	4	34	theme	GPP	537:539	arg1	immunoregulation					517:532	The immunoregulation	513:532	The immunoregulation of GPP	513:539	The immunoregulation of GPP was detected in RAW264.7 macrophages.
31261807	6	35	dep	synthase	876:883	arg1	-α					923:924	-α	923:924	-α	923:924	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	2	36	theme	immune	303:308	arg1	function					310:317	the immune function	299:317	the immune function of a polysaccharide composition consisting of GLP and PUP	299:375	However, the immune function of a polysaccharide composition consisting of GLP and PUP has not been studied.
31261807	8	37	theme	mg/kg	1168:1172	arg1	weight					1160:1165	0, 3.6, 120, 360 mg/kg body weight	1132:1165	0, 3.6, 120, 360 mg/kg body weight (mg/kg BW)	1132:1176	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	8	37	theme	mg/kg	1168:1172	arg1	BW					1174:1175	mg/kg BW	1168:1175	mg/kg BW	1168:1175	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	0	38	theme	Lucidum	30:36	arg1	Composition					5:15	Composition	5:15	Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides	5:95	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	8	39	theme	phagocytic	1020:1029	arg1	function					1031:1038	macrophage phagocytic function	1009:1038	macrophage phagocytic function	1009:1038	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	0	40	theme	Ganoderma	20:28	arg1	Lucidum					30:36	Ganoderma Lucidum	20:36	Ganoderma Lucidum	20:36	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	1	41	theme	Polyporus	214:222	arg1	PUP					252:254	PUP	252:254	PUP	252:254	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	1	41	theme	Polyporus	214:222	arg1	polysaccharides					235:249	Polyporus umbellatus polysaccharides	214:249	Polyporus umbellatus polysaccharides (PUP)	214:255	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	5	42	theme	effect	624:629	arg1	toxicity					600:607	the acute oral toxicity	585:607	the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice	585:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	6	43	theme	oxide	804:808	arg1	production					815:824	nitric oxide (NO) production	797:824	nitric oxide (NO) production	797:824	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	8	44	theme	mg/kg	1149:1153	arg1	weight					1160:1165	0, 3.6, 120, 360 mg/kg body weight	1132:1165	0, 3.6, 120, 360 mg/kg body weight (mg/kg BW)	1132:1176	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	8	44	theme	mg/kg	1149:1153	arg1	BW					1174:1175	mg/kg BW	1168:1175	mg/kg BW	1168:1175	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	6	45	theme	synthase	876:883	arg1	production					815:824	nitric oxide (NO) production	797:824	nitric oxide (NO) production	797:824	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	45	theme	synthase	876:883	arg1	expression					839:848	the mRNA expression	830:848	the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α	830:924	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	8	46	theme	macrophage	1009:1018	arg1	function					1031:1038	macrophage phagocytic function	1009:1038	macrophage phagocytic function	1009:1038	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	0	47	from	Function	121:128	arg1	Mice					133:136	Mice	133:136	Mice	133:136	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	5	48	from	GPP	612:614	arg1	immunoregulation					641:656	immunoregulation	641:656	immunoregulation	641:656	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	5	49	theme	GPP	634:636	arg1	GPP					612:614	GPP	612:614	GPP	612:614	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	5	49	theme	GPP	634:636	arg1	effect					624:629	the effect	620:629	the effect of GPP on immunoregulation	620:656	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	8	50	theme	killer	1068:1073	arg1	cells					1080:1084	natural killer (NK) cells	1060:1084	natural killer (NK) cells	1060:1084	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	2	51	theme	composition	339:349	arg1	function					310:317	the immune function	299:317	the immune function of a polysaccharide composition consisting of GLP and PUP	299:375	However, the immune function of a polysaccharide composition consisting of GLP and PUP has not been studied.
31261807	1	52	theme	umbellatus	224:233	arg1	PUP					252:254	PUP	252:254	PUP	252:254	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	1	52	theme	umbellatus	224:233	arg1	polysaccharides					235:249	Polyporus umbellatus polysaccharides	214:249	Polyporus umbellatus polysaccharides (PUP)	214:255	Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation.
31261807	0	53	dep	Composition	5:15	arg1	Poly-saccharides					80:95	Poly-saccharides	80:95	Poly-saccharides	80:95	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	2	54	theme	polysaccharide	324:337	arg1	composition					339:349	a polysaccharide composition	322:349	a polysaccharide composition consisting of GLP and PUP	322:375	However, the immune function of a polysaccharide composition consisting of GLP and PUP has not been studied.
31261807	8	55	theme	weight	1160:1165	arg1	dose					1124:1127	a dose	1122:1127	a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW)	1122:1176	Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days.
31261807	6	56	theme	phagocytic	777:786	arg1	ability					788:794	phagocytic ability	777:794	phagocytic ability	777:794	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	3	57	theme	3:1	496:498	arg1	ratio					487:491	a ratio	485:491	a ratio of 3:1 (named GPP)	485:510	In this study, we developed a polysaccharide composition consisting of GLP and PUP in a ratio of 3:1 (named GPP).
31261807	3	58	theme	polysaccharide	429:442	arg1	composition					444:454	a polysaccharide composition	427:454	a polysaccharide composition consisting of GLP and PUP	427:480	In this study, we developed a polysaccharide composition consisting of GLP and PUP in a ratio of 3:1 (named GPP).
31261807	5	59	from	effect	624:629	arg1	immunoregulation					641:656	immunoregulation	641:656	immunoregulation	641:656	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	5	60	from	immunoregulation	641:656	arg1	toxicity					600:607	the acute oral toxicity	585:607	the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice	585:664	Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected.
31261807	6	61	theme	nitric	863:868	arg1	iNOS					886:889	iNOS	886:889	iNOS	886:889	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	6	61	theme	nitric	863:868	arg1	synthase					876:883	inducible nitric oxide synthase	853:883	inducible nitric oxide synthase (iNOS)	853:890	The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α.
31261807	0	62	dep	GPP	0:2	arg1	Composition					5:15	Composition	5:15	Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides	5:95	GPP (Composition of Ganoderma Lucidum Poly-saccharides and Polyporus Umbellatus Poly-saccharides) Enhances Innate Immune Function in Mice.
31261807	9	63	theme	GLP	1358:1360	arg1	utilization					1343:1353	utilization	1343:1353	utilization	1343:1353	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	9	63	theme	GLP	1358:1360	arg1	development					1327:1337	further development	1319:1337	further development	1319:1337	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	9	64	from	function	1380:1387	arg1	utilization					1343:1353	utilization	1343:1353	utilization	1343:1353	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	9	64	from	function	1380:1387	arg1	development					1327:1337	further development	1319:1337	further development	1319:1337	Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
31261807	7	65	from	grade	957:961	arg1	mice					966:969	mice	966:969	mice	966:969	GPP belonged to the non-toxic grade in mice.
31394147	9	0	theme	Dubinin-Radushkevich	1176:1195	arg1	model					1197:1201	the Dubinin-Radushkevich model	1172:1201	the Dubinin-Radushkevich model	1172:1201	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	1	1	theme	serious	155:161	arg1	threat					177:182	a serious environmental threat	153:182	a serious environmental threat	153:182	Water contamination with antibiotics is a serious environmental threat.
31394147	1	1	theme	serious	155:161	arg1	contamination					119:131	Water contamination	113:131	Water contamination with antibiotics	113:148	Water contamination with antibiotics is a serious environmental threat.
31394147	10	2	theme	λ-carrageenan	1231:1243	arg1	propensity					1253:1262	the propensity	1249:1262	the propensity of CIP molecules to self-aggregate	1249:1297	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	10	2	theme	λ-carrageenan	1231:1243	arg1	density					1220:1226	The high charge density	1204:1226	The high charge density of λ-carrageenan	1204:1243	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	0	3	theme	carrageenan	71:81	arg1	shells					44:49	siliceous hybrid shells	27:49	siliceous hybrid shells of alginic acid and carrageenan	27:81	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	1	4	theme	environmental	163:175	arg1	threat					177:182	a serious environmental threat	153:182	a serious environmental threat	153:182	Water contamination with antibiotics is a serious environmental threat.
31394147	1	4	theme	environmental	163:175	arg1	contamination					119:131	Water contamination	113:131	Water contamination with antibiotics	113:148	Water contamination with antibiotics is a serious environmental threat.
31394147	9	5	theme	CIP	1053:1055	arg1	adsorption					1057:1066	The CIP adsorption	1049:1066	The CIP adsorption on λ-carrageenan particles	1049:1093	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	8	6	with	consistent	1002:1011	arg1	model					1042:1046	the pseudo-second-order model	1018:1046	the pseudo-second-order model	1018:1046	The kinetic data were consistent with the pseudo-second-order model.
31394147	5	7	theme	contact	663:669	arg1	time					671:674	contact time	663:674	contact time	663:674	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	7	8	theme	CIP	964:966	arg1	removal					953:959	the removal	949:959	the removal of CIP from water	949:977	These high values indicate that these materials are among the most effective sorbents reported so far for the removal of CIP from water.
31394147	10	9	theme	CIP	1267:1269	arg1	molecules					1271:1279	CIP molecules	1267:1279	CIP molecules	1267:1279	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	8	10	theme	pseudo-second-order	1022:1040	arg1	model					1042:1046	the pseudo-second-order model	1018:1046	the pseudo-second-order model	1018:1046	The kinetic data were consistent with the pseudo-second-order model.
31394147	9	11	from	adsorption	1057:1066	arg1	particles					1085:1093	λ-carrageenan particles	1071:1093	λ-carrageenan particles	1071:1093	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	6	12	theme	adsorption	719:728	arg1	464					743:745	464	743:745	464	743:745	The maximum adsorption capacity was 464, 423 and 1350 mg/g for particles prepared from alginic acid, κ- and λ-carrageenan respectively.
31394147	6	12	theme	adsorption	719:728	arg1	capacity					730:737	The maximum adsorption capacity	707:737	The maximum adsorption capacity	707:737	The maximum adsorption capacity was 464, 423 and 1350 mg/g for particles prepared from alginic acid, κ- and λ-carrageenan respectively.
31394147	10	13	theme	charge	1213:1218	arg1	density					1220:1226	The high charge density	1204:1226	The high charge density of λ-carrageenan	1204:1243	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	3	14	theme	alginic	355:361	arg1	acid					363:366	alginic acid	355:366	alginic acid	355:366	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	14	theme	alginic	355:361	arg1	nanosorbents					296:307	silica-based magnetic nanosorbents	274:307	silica-based magnetic nanosorbents prepared using three seaweed polysaccharides	274:352	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	10	15	theme	cooperative	1315:1325	arg1	nature					1327:1332	the cooperative nature	1311:1332	the cooperative nature of CIP adsorption	1311:1350	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	6	16	theme	maximum	711:717	arg1	464					743:745	464	743:745	464	743:745	The maximum adsorption capacity was 464, 423 and 1350 mg/g for particles prepared from alginic acid, κ- and λ-carrageenan respectively.
31394147	6	16	theme	maximum	711:717	arg1	capacity					730:737	The maximum adsorption capacity	707:737	The maximum adsorption capacity	707:737	The maximum adsorption capacity was 464, 423 and 1350 mg/g for particles prepared from alginic acid, κ- and λ-carrageenan respectively.
31394147	0	17	theme	ciprofloxacin	98:110	arg1	removal					87:93	removal	87:93	removal of ciprofloxacin	87:110	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	6	18	theme	alginic	794:800	arg1	acid					802:805	alginic acid	794:805	alginic acid	794:805	The maximum adsorption capacity was 464, 423 and 1350 mg/g for particles prepared from alginic acid, κ- and λ-carrageenan respectively.
31394147	7	19	theme	high	849:852	arg1	values					854:859	These high values	843:859	These high values	843:859	These high values indicate that these materials are among the most effective sorbents reported so far for the removal of CIP from water.
31394147	3	20	theme	ciprofloxacin	437:449	arg1	uptake					427:432	the uptake	423:432	the uptake of ciprofloxacin	423:449	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	21	theme	seaweed	330:336	arg1	polysaccharides					338:352	three seaweed polysaccharides	324:352	three seaweed polysaccharides	324:352	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	8	22	theme	kinetic	984:990	arg1	data					992:995	The kinetic data	980:995	The kinetic data	980:995	The kinetic data were consistent with the pseudo-second-order model.
31394147	8	22	theme	kinetic	984:990	arg1	consistent					1002:1011	consistent	1002:1011	consistent	1002:1011	The kinetic data were consistent with the pseudo-second-order model.
31394147	11	23	theme	properties	1499:1508	arg1	loss					1480:1483	a significant loss	1466:1483	a significant loss of adsorptive properties	1466:1508	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31394147	0	24	theme	Magnetic	0:7	arg1	nanosorbents					9:20	Magnetic nanosorbents	0:20	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.	0:111	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	1	25	with	contamination	119:131	arg1	antibiotics					138:148	antibiotics	138:148	antibiotics	138:148	Water contamination with antibiotics is a serious environmental threat.
31394147	2	26	from	antibiotics	244:254	arg1	water					259:263	water	259:263	water	259:263	Ciprofloxacin (CIP) is one of the most frequently detected antibiotics in water.
31394147	0	27	theme	siliceous	27:35	arg1	shells					44:49	siliceous hybrid shells	27:49	siliceous hybrid shells of alginic acid and carrageenan	27:81	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	5	28	theme	adsorption	600:609	arg1	performance					611:621	the adsorption performance	596:621	the adsorption performance towards CIP	596:633	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	9	29	theme	cooperative	1106:1116	arg1	process					1118:1124	a cooperative process	1104:1124	a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model	1104:1201	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	3	30	theme	silica-based	274:285	arg1	nanosorbents					296:307	silica-based magnetic nanosorbents	274:307	silica-based magnetic nanosorbents prepared using three seaweed polysaccharides	274:352	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	30	theme	silica-based	274:285	arg1	κ-					369:370	κ-	369:370	κ-	369:370	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	30	theme	silica-based	274:285	arg1	λ-carrageenan					376:388	λ-carrageenan	376:388	λ-carrageenan	376:388	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	30	theme	silica-based	274:285	arg1	acid					363:366	alginic acid	355:366	alginic acid	355:366	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	9	31	with	process	1118:1124	arg1	isotherm					1141:1148	sigmoidal isotherm	1131:1148	sigmoidal isotherm	1131:1148	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	10	32	theme	adsorption	1341:1350	arg1	nature					1327:1332	the cooperative nature	1311:1332	the cooperative nature of CIP adsorption	1311:1350	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	9	33	theme	λ-carrageenan	1071:1083	arg1	particles					1085:1093	λ-carrageenan particles	1071:1093	λ-carrageenan particles	1071:1093	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	10	34	theme	molecules	1271:1279	arg1	propensity					1253:1262	the propensity	1249:1262	the propensity of CIP molecules to self-aggregate	1249:1297	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	10	34	theme	molecules	1271:1279	arg1	density					1220:1226	The high charge density	1204:1226	The high charge density of λ-carrageenan	1204:1243	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	11	35	theme	adsorptive	1488:1497	arg1	properties					1499:1508	adsorptive properties	1488:1508	adsorptive properties	1488:1508	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31394147	0	36	theme	hybrid	37:42	arg1	shells					44:49	siliceous hybrid shells	27:49	siliceous hybrid shells of alginic acid and carrageenan	27:81	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	11	37	theme	mild	1393:1396	arg1	conditions					1398:1407	mild conditions	1393:1407	mild conditions	1393:1407	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31394147	5	38	theme	systematic	548:557	arg1	investigation					559:571	A systematic investigation	546:571	A systematic investigation	546:571	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	0	39	theme	alginic	54:60	arg1	acid					62:65	alginic acid	54:65	alginic acid	54:65	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	11	40	theme	CIP	1432:1434	arg1	removal					1436:1442	CIP removal	1432:1442	CIP removal	1432:1442	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31394147	3	41	theme	magnetic	287:294	arg1	nanosorbents					296:307	silica-based magnetic nanosorbents	274:307	silica-based magnetic nanosorbents prepared using three seaweed polysaccharides	274:352	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	41	theme	magnetic	287:294	arg1	κ-					369:370	κ-	369:370	κ-	369:370	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	41	theme	magnetic	287:294	arg1	λ-carrageenan					376:388	λ-carrageenan	376:388	λ-carrageenan	376:388	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	3	41	theme	magnetic	287:294	arg1	acid					363:366	alginic acid	355:366	alginic acid	355:366	Herein, silica-based magnetic nanosorbents prepared using three seaweed polysaccharides, alginic acid, κ- and λ-carrageenan, were developed and evaluated in the uptake of ciprofloxacin.
31394147	1	42	theme	Water	113:117	arg1	threat					177:182	a serious environmental threat	153:182	a serious environmental threat	153:182	Water contamination with antibiotics is a serious environmental threat.
31394147	1	42	theme	Water	113:117	arg1	contamination					119:131	Water contamination	113:131	Water contamination with antibiotics	113:148	Water contamination with antibiotics is a serious environmental threat.
31394147	5	43	theme	initial	680:686	arg1	concentration					692:704	initial CIP concentration	680:704	initial CIP concentration	680:704	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	0	44	with	nanosorbents	9:20	arg1	shells					44:49	siliceous hybrid shells	27:49	siliceous hybrid shells of alginic acid and carrageenan	27:81	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	5	45	theme	initial	651:657	arg1	pH					659:660	the initial pH	647:660	the initial pH	647:660	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	7	46	theme	effective	910:918	arg1	sorbents					920:927	the most effective sorbents	901:927	the most effective sorbents reported so far for the removal of CIP from water	901:977	These high values indicate that these materials are among the most effective sorbents reported so far for the removal of CIP from water.
31394147	10	47	dep	propensity	1253:1262	arg1	self-aggregate					1284:1297	self-aggregate	1284:1297	to self-aggregate	1281:1297	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	5	48	theme	CIP	688:690	arg1	concentration					692:704	initial CIP concentration	680:704	initial CIP concentration	680:704	A systematic investigation was conducted to study the adsorption performance towards CIP, by varying the initial pH, contact time and initial CIP concentration.
31394147	10	49	theme	high	1208:1211	arg1	density					1220:1226	The high charge density	1204:1226	The high charge density of λ-carrageenan	1204:1243	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	9	50	theme	sigmoidal	1131:1139	arg1	isotherm					1141:1148	sigmoidal isotherm	1131:1148	sigmoidal isotherm	1131:1148	The CIP adsorption on λ-carrageenan particles followed a cooperative process with sigmoidal isotherm that was described by the Dubinin-Radushkevich model.
31394147	10	51	theme	CIP	1337:1339	arg1	adsorption					1341:1350	CIP adsorption	1337:1350	CIP adsorption	1337:1350	The high charge density of λ-carrageenan and the propensity of CIP molecules to self-aggregate may explain the cooperative nature of CIP adsorption.
31394147	11	52	dep	4	1450:1450	arg1	to					1447:1448	to	1447:1448	to	1447:1448	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31394147	2	53	theme	antibiotics	244:254	arg1	antibiotics					244:254	the most frequently detected antibiotics	215:254	the most frequently detected antibiotics in water	215:263	Ciprofloxacin (CIP) is one of the most frequently detected antibiotics in water.
31394147	2	53	theme	antibiotics	244:254	arg1	one					208:210	one	208:210	one	208:210	Ciprofloxacin (CIP) is one of the most frequently detected antibiotics in water.
31394147	7	54	from	water	973:977	arg1	removal					953:959	the removal	949:959	the removal of CIP from water	949:977	These high values indicate that these materials are among the most effective sorbents reported so far for the removal of CIP from water.
31394147	0	55	theme	acid	62:65	arg1	shells					44:49	siliceous hybrid shells	27:49	siliceous hybrid shells of alginic acid and carrageenan	27:81	Magnetic nanosorbents with siliceous hybrid shells of alginic acid and carrageenan for removal of ciprofloxacin.
31394147	2	56	theme	detected	235:242	arg1	antibiotics					244:254	the most frequently detected antibiotics	215:254	the most frequently detected antibiotics in water	215:263	Ciprofloxacin (CIP) is one of the most frequently detected antibiotics in water.
31394147	11	57	theme	significant	1468:1478	arg1	loss					1480:1483	a significant loss	1466:1483	a significant loss of adsorptive properties	1466:1508	The sorbents were easily regenerated in mild conditions and could be reused in CIP removal up to 4 times without a significant loss of adsorptive properties.
31849912	6	0	theme	protein	1195:1201	arg1	glycosylation					1203:1215	O-linked protein glycosylation	1186:1215	O-linked protein glycosylation	1186:1215	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	2	1	theme	factors	372:378	arg1	identification					354:367	the identification	350:367	the identification of factors impacting on protein secretion	350:409	Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology.
31849912	2	1	theme	factors	372:378	arg1	important					414:422	important	414:422	important	414:422	Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology.
31849912	1	2	theme	various	267:273	arg1	effectors					289:297	various proteinaceous effectors	267:297	various proteinaceous effectors	267:297	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	4	3	link	O-linked	799:806	arg1	glycosylation					816:828	O-linked protein glycosylation	799:828	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	8	4	theme	proteins	1480:1487	arg1	secretion					1467:1475	the secretion	1463:1475	the secretion of proteins	1463:1487	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	3	5	theme	relaxed	596:602	arg1	specificity					611:621	relaxed glycan specificity	596:621	relaxed glycan specificity	596:621	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	5	6	gly	glycosylation	912:924	arg1	chaperones					982:991	periplasmic chaperones	970:991	periplasmic chaperones such as DegP	970:1004	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	5	6	gly	glycosylation	912:924	arg1	proteins					950:957	multiple V. cholerae proteins	929:957	multiple V. cholerae proteins	929:957	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	7	7	with	protein	1241:1247	arg1	similarities					1265:1276	structural similarities	1254:1276	structural similarities to PglLVc	1254:1286	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	7	7	with	protein	1241:1247	arg1	oligosaccharyltransferases					1310:1335	other established oligosaccharyltransferases	1292:1335	other established oligosaccharyltransferases (OTases)	1292:1344	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	7	7	with	protein	1241:1247	arg1	OTases					1338:1343	OTases	1338:1343	OTases	1338:1343	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	4	8	theme	defined	713:719	arg1	precursor					764:772	a defined, undecaprenyl diphosphate-linked glycoform precursor	711:772	a defined, undecaprenyl diphosphate-linked glycoform precursor	711:772	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	10	9	theme	secretion	1836:1844	arg1	spectrum					1750:1757	broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion	1744:1844	spectrum	1750:1757	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	10	9	theme	secretion	1836:1844	arg1	efficacy					1798:1805	the efficacy	1794:1805	the efficacy of type II-dependent protein secretion	1794:1844	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	1	10	theme	bacterial	172:180	arg1	pathogens					182:190	bacterial pathogens	172:190	bacterial pathogens	172:190	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	9	11	theme	cholera	1561:1567	arg1	CT					1576:1577	CT	1576:1577	CT	1576:1577	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	9	11	theme	cholera	1561:1567	arg1	toxin					1569:1573	cholera toxin	1561:1573	altered cholera toxin (CT) secretion	1553:1588	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	0	12	from	Secretion	73:81	arg1	cholerae					119:126	Vibrio cholerae	112:126	Vibrio cholerae	112:126	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	7	13	theme	structural	1254:1263	arg1	similarities					1265:1276	structural similarities	1254:1276	structural similarities to PglLVc	1254:1286	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	1	14	theme	human-pathogen	220:233	arg1	cholerae					242:249	facultative human-pathogen Vibrio cholerae	208:249	facultative human-pathogen Vibrio cholerae	208:249	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	4	15	dep	V.	680:681	arg1	cholerae					683:690	cholerae	683:690	cholerae	683:690	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	3	16	theme	Escherichia	640:650	arg1	system					657:662	an engineered Escherichia coli system	626:662	an engineered Escherichia coli system	626:662	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	4	17	theme	O-linked	799:806	arg1	glycosylation					816:828	O-linked protein glycosylation	799:828	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	6	18	theme	complementation	1107:1121	arg1	strains					1123:1129	complementation strains	1107:1129	complementation strains	1107:1129	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	9	19	located	observed	1632:1639	arg1	mutants					1680:1686	ΔrbmD single or double mutants	1657:1686	ΔrbmD single or double mutants	1657:1686	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	9	19	located	observed	1632:1639	arg1	Vc					1650:1651	ΔpglL Vc	1644:1651	ΔpglL Vc	1644:1651	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	9	19	located	observed	1632:1639	arg2	secretion					1580:1588	altered cholera toxin (CT) secretion	1553:1588	altered cholera toxin (CT) secretion	1553:1588	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	4	20	theme	glycoform	754:762	arg1	precursor					764:772	a defined, undecaprenyl diphosphate-linked glycoform precursor	711:772	a defined, undecaprenyl diphosphate-linked glycoform precursor	711:772	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	5	21	theme	periplasmic	970:980	arg1	chaperones					982:991	periplasmic chaperones	970:991	periplasmic chaperones such as DegP	970:1004	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	5	21	theme	periplasmic	970:980	arg1	DegP					1001:1004	DegP	1001:1004	DegP	1001:1004	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	6	22	gly	glycosylation	1203:1215	arg1	V.					1220:1221	V. cholerae	1220:1230	V. cholerae	1220:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	10	23	theme	O-linked	1759:1766	arg1	glycosylation					1776:1788	O-linked protein glycosylation	1759:1788	O-linked protein glycosylation	1759:1788	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	9	24	theme	biofilm	1614:1620	arg1	formation					1622:1630	biofilm formation	1614:1630	biofilm formation	1614:1630	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	6	25	theme	defined	1078:1084	arg1	mutants					1095:1101	deletion mutants	1086:1101	deletion mutants	1086:1101	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	5	26	theme	efficient	1029:1037	arg1	secretion					1057:1065	efficient type II-dependent secretion	1029:1065	efficient type II-dependent secretion	1029:1065	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	0	27	theme	Associated	87:96	arg1	Phenotypes					98:107	Associated Phenotypes	87:107	Associated Phenotypes in Vibrio cholerae	87:126	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	5	28	theme	II-dependent	1044:1055	arg1	secretion					1057:1065	efficient type II-dependent secretion	1029:1065	efficient type II-dependent secretion	1029:1065	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	2	29	theme	bacterial	442:450	arg1	pathophysiology					452:466	the bacterial pathophysiology	438:466	the bacterial pathophysiology	438:466	Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology.
31849912	7	30	theme	other	1292:1296	arg1	OTases					1338:1343	OTases	1338:1343	OTases	1338:1343	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	7	30	theme	other	1292:1296	arg1	oligosaccharyltransferases					1310:1335	other established oligosaccharyltransferases	1292:1335	other established oligosaccharyltransferases (OTases)	1292:1344	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	9	31	theme	ΔpglL	1644:1648	arg1	Vc					1650:1651	ΔpglL Vc	1644:1651	ΔpglL Vc	1644:1651	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	10	32	theme	critical	1846:1853	arg1	spectrum					1750:1757	broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion	1744:1844	spectrum	1750:1757	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	4	33	theme	glycosylation	816:828	arg1	activity					830:837	functional O-linked protein glycosylation activity	788:837	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	10	34	theme	unique	1718:1723	arg1	connection					1725:1734	a unique connection	1716:1734	a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle	1716:1881	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	10	35	theme	protein	1768:1774	arg1	glycosylation					1776:1788	O-linked protein glycosylation	1759:1788	O-linked protein glycosylation	1759:1788	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	0	36	theme	Spectrum	8:15	arg1	System					39:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	10	37	theme	protein	1828:1834	arg1	secretion					1836:1844	type II-dependent protein secretion	1810:1844	type II-dependent protein secretion	1810:1844	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	9	38	theme	single	1663:1668	arg1	mutants					1680:1686	ΔrbmD single or double mutants	1657:1686	ΔrbmD single or double mutants	1657:1686	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	10	39	dep	spectrum	1750:1757	arg1	glycosylation					1776:1788	O-linked protein glycosylation	1759:1788	O-linked protein glycosylation	1759:1788	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	3	40	theme	predicted	479:487	arg1	oligosaccharyltransferase					489:513	a predicted oligosaccharyltransferase	477:513	a predicted oligosaccharyltransferase of V. cholerae	477:528	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	3	40	theme	predicted	479:487	arg1	PglLVc					469:474	PglLVc	469:474	PglLVc	469:474	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	0	41	theme	Glycosylation	25:37	arg1	System					39:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	9	42	theme	double	1673:1678	arg1	mutants					1680:1686	ΔrbmD single or double mutants	1657:1686	ΔrbmD single or double mutants	1657:1686	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	5	43	dep	V.	938:939	arg1	cholerae					941:948	cholerae	941:948	cholerae	941:948	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	6	44	link	O-linked	1186:1193	arg1	glycosylation					1203:1215	O-linked protein glycosylation	1186:1215	O-linked protein glycosylation	1186:1215	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	3	45	theme	cholerae	521:528	arg1	oligosaccharyltransferase					489:513	a predicted oligosaccharyltransferase	477:513	a predicted oligosaccharyltransferase of V. cholerae	477:528	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	3	45	theme	cholerae	521:528	arg1	PglLVc					469:474	PglLVc	469:474	PglLVc	469:474	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	1	46	theme	Vibrio	235:240	arg1	cholerae					242:249	facultative human-pathogen Vibrio cholerae	208:249	facultative human-pathogen Vibrio cholerae	208:249	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	7	47	theme	phenotypical	1373:1384	arg1	characterization					1386:1401	this phenotypical characterization	1368:1401	this phenotypical characterization	1368:1401	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	10	48	link	O-linked	1759:1766	arg1	glycosylation					1776:1788	O-linked protein glycosylation	1759:1788	O-linked protein glycosylation	1759:1788	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	2	49	theme	protein	393:399	arg1	secretion					401:409	protein secretion	393:409	protein secretion	393:409	Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology.
31849912	3	50	dep	Escherichia	640:650	arg1	coli					652:655	coli	652:655	coli	652:655	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	6	51	theme	glycosylation	1203:1215	arg1	role					1178:1181	the physiological role	1160:1181	the physiological role of O-linked protein glycosylation in V. cholerae	1160:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	10	52	theme	type	1810:1813	arg1	secretion					1836:1844	type II-dependent protein secretion	1810:1844	type II-dependent protein secretion	1810:1844	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	6	53	theme	O-linked	1186:1193	arg1	glycosylation					1203:1215	O-linked protein glycosylation	1186:1215	O-linked protein glycosylation	1186:1215	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	1	54	theme	proteinaceous	275:287	arg1	effectors					289:297	various proteinaceous effectors	267:297	various proteinaceous effectors	267:297	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	6	55	from	role	1178:1181	arg1	cholerae					1223:1230	V. cholerae	1220:1230	V. cholerae	1220:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	3	56	theme	O-glycosylation	566:580	arg1	activity					582:589	O-glycosylation activity	566:589	O-glycosylation activity	566:589	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	5	57	theme	V.	938:939	arg1	chaperones					982:991	periplasmic chaperones	970:991	periplasmic chaperones such as DegP	970:1004	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	5	57	theme	V.	938:939	arg1	proteins					950:957	multiple V. cholerae proteins	929:957	multiple V. cholerae proteins	929:957	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	5	58	theme	proteins	950:957	arg1	glycosylation					912:924	the glycosylation	908:924	the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion	908:1065	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	3	59	theme	glycan	604:609	arg1	specificity					611:621	relaxed glycan specificity	596:621	relaxed glycan specificity	596:621	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	9	60	theme	altered	1553:1559	arg1	secretion					1580:1588	altered cholera toxin (CT) secretion	1553:1588	altered cholera toxin (CT) secretion	1553:1588	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	9	61	theme	toxin	1569:1573	arg1	secretion					1580:1588	altered cholera toxin (CT) secretion	1553:1588	altered cholera toxin (CT) secretion	1553:1588	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	3	62	theme	engineered	629:638	arg1	system					657:662	an engineered Escherichia coli system	626:662	an engineered Escherichia coli system	626:662	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	1	63	theme	Protein	129:135	arg1	secretion					137:145	Protein secretion	129:145	Protein secretion	129:145	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	0	64	from	Phenotypes	98:107	arg1	cholerae					119:126	Vibrio cholerae	112:126	Vibrio cholerae	112:126	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	4	65	theme	functional	788:797	arg1	activity					830:837	functional O-linked protein glycosylation activity	788:837	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	0	66	theme	Protein	65:71	arg1	Secretion					73:81	Type II Protein Secretion	57:81	Type II Protein Secretion	57:81	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	9	67	theme	chitin	1591:1596	arg1	utilization					1598:1608	chitin utilization	1591:1608	chitin utilization	1591:1608	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	1	68	theme	crucial	155:161	arg1	role					163:166	a crucial role	153:166	a crucial role	153:166	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	6	69	theme	deletion	1086:1093	arg1	mutants					1095:1101	deletion mutants	1086:1101	deletion mutants	1086:1101	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	8	70	theme	PglLVc	1439:1444	arg1	presence					1416:1423	presence	1416:1423	presence	1416:1423	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	8	70	theme	PglLVc	1439:1444	arg1	absence					1428:1434	absence	1428:1434	absence	1428:1434	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	7	71	theme	established	1298:1308	arg1	OTases					1338:1343	OTases	1338:1343	OTases	1338:1343	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	7	71	theme	established	1298:1308	arg1	oligosaccharyltransferases					1310:1335	other established oligosaccharyltransferases	1292:1335	other established oligosaccharyltransferases (OTases)	1292:1344	RbmD, a protein with structural similarities to PglLVc and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization.
31849912	5	72	theme	type	1039:1042	arg1	secretion					1057:1065	efficient type II-dependent secretion	1029:1065	efficient type II-dependent secretion	1029:1065	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	8	73	theme	RbmD	1450:1453	arg1	presence					1416:1423	presence	1416:1423	presence	1416:1423	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	8	73	theme	RbmD	1450:1453	arg1	absence					1428:1434	absence	1428:1434	absence	1428:1434	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	4	74	theme	PglLVc	842:847	arg1	activity					830:837	functional O-linked protein glycosylation activity	788:837	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	6	75	theme	V.	1220:1221	arg1	cholerae					1223:1230	V. cholerae	1220:1230	V. cholerae	1220:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	0	76	theme	Vibrio	112:117	arg1	cholerae					119:126	Vibrio cholerae	112:126	Vibrio cholerae	112:126	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	4	77	theme	V.	852:853	arg1	cholerae					855:862	V. cholerae	852:862	V. cholerae	852:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	0	78	theme	Broad	2:6	arg1	Spectrum					8:15	A Broad Spectrum	0:15	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	4	79	from	activity	830:837	arg1	cholerae					855:862	V. cholerae	852:862	V. cholerae	852:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	6	80	theme	physiological	1164:1176	arg1	role					1178:1181	the physiological role	1160:1181	the physiological role of O-linked protein glycosylation in V. cholerae	1160:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	4	81	theme	protein	808:814	arg1	glycosylation					816:828	O-linked protein glycosylation	799:828	functional O-linked protein glycosylation activity of PglLVc in V. cholerae	788:862	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	0	82	theme	Protein	17:23	arg1	System					39:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System	0:44	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	1	83	theme	different	302:310	arg1	stages					312:317	different stages	302:317	different stages of its lifecycle	302:334	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	9	84	theme	ΔrbmD	1657:1661	arg1	mutants					1680:1686	ΔrbmD single or double mutants	1657:1686	ΔrbmD single or double mutants	1657:1686	This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in ΔpglL Vc and ΔrbmD single or double mutants.
31849912	3	85	theme	V.	518:519	arg1	cholerae					521:528	V. cholerae	518:528	V. cholerae	518:528	PglLVc, a predicted oligosaccharyltransferase of V. cholerae, has been recently shown to exhibit O-glycosylation activity with relaxed glycan specificity in an engineered Escherichia coli system.
31849912	1	86	theme	facultative	208:218	arg1	cholerae					242:249	facultative human-pathogen Vibrio cholerae	208:249	facultative human-pathogen Vibrio cholerae	208:249	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	6	87	theme	first	1140:1144	arg1	insights					1146:1153	first insights	1140:1153	first insights into the physiological role of O-linked protein glycosylation in V. cholerae	1140:1230	Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of O-linked protein glycosylation in V. cholerae.
31849912	8	88	theme	type	1497:1500	arg1	T2SS					1523:1526	T2SS	1523:1526	T2SS	1523:1526	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	8	88	theme	type	1497:1500	arg1	system					1515:1520	the type II secretion system	1493:1520	the type II secretion system (T2SS)	1493:1527	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	10	89	theme	broad	1744:1748	arg1	spectrum					1750:1757	broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion	1744:1844	spectrum	1750:1757	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
31849912	0	90	theme	Type	57:60	arg1	Secretion					73:81	Type II Protein Secretion	57:81	Type II Protein Secretion	57:81	A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae.
31849912	1	91	theme	lifecycle	326:334	arg1	stages					312:317	different stages	302:317	different stages of its lifecycle	302:334	Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle.
31849912	8	92	theme	secretion	1505:1513	arg1	T2SS					1523:1526	T2SS	1523:1526	T2SS	1523:1526	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	8	92	theme	secretion	1505:1513	arg1	system					1515:1520	the type II secretion system	1493:1520	the type II secretion system (T2SS)	1493:1527	Remarkably, presence or absence of PglLVc and RbmD impacts the secretion of proteins via the type II secretion system (T2SS).
31849912	5	93	theme	multiple	929:936	arg1	chaperones					982:991	periplasmic chaperones	970:991	periplasmic chaperones such as DegP	970:1004	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	5	93	theme	multiple	929:936	arg1	proteins					950:957	multiple V. cholerae proteins	929:957	multiple V. cholerae proteins	929:957	We demonstrate that PglLVc is required for the glycosylation of multiple V. cholerae proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion.
31849912	4	94	dep	defined	713:719	arg1	diphosphate-linked					735:752	diphosphate-linked	735:752	diphosphate-linked	735:752	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	4	95	theme	V.	680:681	arg1	strains					692:698	V. cholerae strains	680:698	V. cholerae strains	680:698	By engineering V. cholerae strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional O-linked protein glycosylation activity of PglLVc in V. cholerae.
31849912	10	96	theme	II-dependent	1815:1826	arg1	secretion					1836:1844	type II-dependent protein secretion	1810:1844	type II-dependent protein secretion	1810:1844	This work thus establishes a unique connection between broad spectrum O-linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen's lifecycle.
30569218	7	0	theme	Deinococci	1184:1193	arg1	growth					1140:1145	the growth	1136:1145	the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus	1136:1237	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	9	1	theme	glucosamine	1488:1498	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	4	2	theme	gas	694:696	arg1	chromatography					698:711	gas chromatography	694:711	gas chromatography	694:711	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	4	3	theme	bifidobacteria	566:579	arg1	EPSs					581:584	bifidobacteria EPSs	566:584	bifidobacteria EPSs	566:584	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	8	4	theme	acid	1288:1291	arg1	production					1264:1273	the production	1260:1273	the production of propionic acid	1260:1291	EPSs also increased the production of propionic acid compared to the starch group.
30569218	5	5	contain	has	759:761	arg2	capacity					767:774	the capacity to degrade EPSs	763:790	the capacity to degrade EPSs	763:790	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	5	5	contain	has	759:761	arg1	microbiota					748:757	human gut microbiota	738:757	human gut microbiota	738:757	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	9	6	contain	had	1429:1431	arg1	EPSs					1424:1427	EPSs	1424:1427	EPSs	1424:1427	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	6	contain	had	1429:1431	arg2	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	7	theme	absorption	1433:1442	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	3	8	theme	microbial	490:498	arg1	ecosystem					500:508	the microbial ecosystem	486:508	the microbial ecosystem	486:508	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	9	9	theme	chromatography	1390:1403	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	0	10	from	Effects	0:6	arg1	microbiota					67:76	human gut microbiota	57:76	human gut microbiota in vitro	57:85	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	9	11	theme	ribose	1514:1519	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	12	theme	system	1405:1410	arg1	results					1337:1343	The detection results	1323:1343	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system	1323:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	7	13	theme	class	1178:1182	arg1	Deinococci					1184:1193	class Deinococci	1178:1193	class Deinococci	1178:1193	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	0	14	from	microbiota	67:76	arg1	vitro					81:85	vitro	81:85	vitro	81:85	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	8	15	theme	starch	1309:1314	arg1	group					1316:1320	the starch group	1305:1320	the starch group	1305:1320	EPSs also increased the production of propionic acid compared to the starch group.
30569218	3	16	contain	has	416:418	arg2	benefits					420:427	benefits	420:427	benefits in food and health	420:446	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	3	16	contain	has	416:418	arg1	production					396:405	EPS production	392:405	EPS production	392:405	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	5	17	theme	other	879:883	arg1	hand					885:888	the other hand	875:888	the other hand	875:888	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	2	18	from	applications	230:241	arg1	sectors					254:260	diverse sectors	246:260	diverse sectors	246:260	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	6	19	theme	VI_Bif	956:961	arg1	group					963:967	the VI_Bif group	952:967	the VI_Bif group	952:967	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	5	20	theme	human	738:742	arg1	microbiota					748:757	human gut microbiota	738:757	human gut microbiota	738:757	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	9	21	theme	rhamnose	1461:1468	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	4	22	theme	16S	653:655	arg1	sequencing					678:687	16S rDNA high-throughput sequencing	653:687	16S rDNA high-throughput sequencing	653:687	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	5	23	theme	gut	744:746	arg1	microbiota					748:757	human gut microbiota	738:757	human gut microbiota	738:757	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	7	24	theme	bifidobacteria	1108:1121	arg1	EPS					1123:1125	The bifidobacteria EPS	1104:1125	The bifidobacteria EPS	1104:1125	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	3	25	from	effects	461:467	arg1	probiotics					472:481	probiotics	472:481	probiotics in the microbial ecosystem	472:508	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	5	26	dep	EPSs	891:894	arg1	regulate					896:903	regulate	896:903	regulate the human gut microbiota	896:928	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	6	27	theme	Fermented	931:939	arg1	samples					941:947	Fermented samples	931:947	Fermented samples in the VI_Bif group	931:967	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	1	28	attach	present	165:171	arg2	Exopolysaccharides					88:105	Exopolysaccharides	88:105	Exopolysaccharides (EPSs)	88:112	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	1	28	attach	present	165:171	arg1	surface					180:186	the surface	176:186	the surface of bifidobacteria	176:204	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	1	28	attach	present	165:171	arg2	polymers					131:138	carbohydrate polymers	118:138	carbohydrate polymers that are synthesized and present on the surface of bifidobacteria	118:204	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	5	29	theme	human	909:913	arg1	microbiota					919:928	the human gut microbiota	905:928	the human gut microbiota	905:928	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	4	30	theme	high-throughput	662:676	arg1	sequencing					678:687	16S rDNA high-throughput sequencing	653:687	16S rDNA high-throughput sequencing	653:687	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	3	31	theme	EPS	392:394	arg1	production					396:405	EPS production	392:405	EPS production	392:405	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	9	32	theme	molecular	1530:1538	arg1	proportion					1540:1549	the molecular proportion	1526:1549	the molecular proportion of these monosaccharides	1526:1574	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	32	theme	molecular	1530:1538	arg1	3					1605:1605	3	1605:1605	3	1605:1605	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	3	33	from	probiotics	472:481	arg1	ecosystem					500:508	the microbial ecosystem	486:508	the microbial ecosystem	486:508	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	5	34	theme	gut	915:917	arg1	microbiota					919:928	the human gut microbiota	905:928	the human gut microbiota	905:928	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	2	35	theme	more	376:379	arg1	attention					381:389	more attention	376:389	more attention	376:389	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	0	36	theme	exopolysaccharides	35:52	arg1	Effects					0:6	Effects	0:6	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.	0:86	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	6	37	from	samples	941:947	arg1	group					963:967	the VI_Bif group	952:967	the VI_Bif group	952:967	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	9	38	theme	monosaccharides	1560:1574	arg1	proportion					1540:1549	the molecular proportion	1526:1549	the molecular proportion of these monosaccharides	1526:1574	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	38	theme	monosaccharides	1560:1574	arg1	3					1605:1605	3	1605:1605	3	1605:1605	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	0	39	theme	bifidobacteria-produced	11:33	arg1	exopolysaccharides					35:52	bifidobacteria-produced exopolysaccharides	11:52	bifidobacteria-produced exopolysaccharides	11:52	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	9	40	theme	galactose/acetyl	1471:1486	arg1	glucosamine					1488:1498	galactose/acetyl glucosamine	1471:1498	galactose/acetyl glucosamine	1471:1498	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	10	41	theme	bifidobacteria	1711:1724	arg1	EPS					1726:1728	bifidobacteria EPS	1711:1728	bifidobacteria EPS	1711:1728	The monosaccharide composition of this EPS appears to be more complex than previously reported for bifidobacteria EPS.
30569218	10	42	theme	monosaccharide	1616:1629	arg1	complex					1674:1680	complex	1674:1680	complex	1674:1680	The monosaccharide composition of this EPS appears to be more complex than previously reported for bifidobacteria EPS.
30569218	10	42	theme	monosaccharide	1616:1629	arg1	composition					1631:1641	The monosaccharide composition	1612:1641	The monosaccharide composition of this EPS	1612:1653	The monosaccharide composition of this EPS appears to be more complex than previously reported for bifidobacteria EPS.
30569218	0	43	theme	human	57:61	arg1	microbiota					67:76	human gut microbiota	57:76	human gut microbiota in vitro	57:85	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	6	44	theme	carbon	1089:1094	arg1	starch					1067:1072	starch	1067:1072	starch	1067:1072	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	6	44	theme	carbon	1089:1094	arg1	source					1096:1101	a carbon source	1087:1101	a carbon source	1087:1101	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	2	45	theme	diverse	246:252	arg1	sectors					254:260	diverse sectors	246:260	diverse sectors	246:260	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	1	46	theme	bifidobacteria	191:204	arg1	surface					180:186	the surface	176:186	the surface of bifidobacteria	176:204	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	9	47	theme	fucose	1453:1458	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	48	theme	detection	1327:1335	arg1	results					1337:1343	The detection results	1323:1343	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system	1323:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	11	49	theme	Additional	1731:1740	arg1	studies					1742:1748	Additional studies	1731:1748	Additional studies	1731:1748	Additional studies are needed to elucidate its structure and functions.
30569218	3	50	contain	has	457:459	arg2	effects					461:467	effects	461:467	effects on probiotics in the microbial ecosystem	461:508	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	3	50	contain	has	457:459	arg1	production					396:405	EPS production	392:405	EPS production	392:405	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	5	51	theme	degradation	806:816	arg1	%					843:843	approximately 50%	827:843	approximately 50%	827:843	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	5	51	theme	degradation	806:816	arg1	rate					818:821	the degradation rate	802:821	the degradation rate	802:821	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	9	52	theme	ion	1386:1388	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	4	53	theme	thin-layer	626:635	arg1	chromatography					637:650	thin-layer chromatography	626:650	thin-layer chromatography	626:650	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	1	54	theme	carbohydrate	118:129	arg1	Exopolysaccharides					88:105	Exopolysaccharides	88:105	Exopolysaccharides (EPSs)	88:112	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	1	54	theme	carbohydrate	118:129	arg1	polymers					131:138	carbohydrate polymers	118:138	carbohydrate polymers that are synthesized and present on the surface of bifidobacteria	118:204	Exopolysaccharides (EPSs) are carbohydrate polymers that are synthesized and present on the surface of bifidobacteria.
30569218	0	55	theme	gut	63:65	arg1	microbiota					67:76	human gut microbiota	57:76	human gut microbiota in vitro	57:85	Effects of bifidobacteria-produced exopolysaccharides on human gut microbiota in vitro.
30569218	7	56	theme	Deinococcus_Thermus	1157:1175	arg1	growth					1140:1145	the growth	1136:1145	the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus	1136:1237	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	2	57	theme	potential	220:228	arg1	biotechnology					277:289	biotechnology	277:289	biotechnology	277:289	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	2	57	theme	potential	220:228	arg1	food					271:274	food	271:274	food	271:274	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	2	57	theme	potential	220:228	arg1	applications					230:241	their potential applications	214:241	their potential applications	214:241	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	2	57	theme	potential	220:228	arg1	cosmetics					292:300	cosmetics	292:300	cosmetics	292:300	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	2	57	theme	potential	220:228	arg1	medicine					307:314	medicine	307:314	medicine	307:314	Due to their potential applications in diverse sectors, such as food, biotechnology, cosmetics, and medicine, EPSs synthesized by bifidobacteria have recently attracted more attention.
30569218	4	58	theme	rDNA	657:660	arg1	sequencing					678:687	16S rDNA high-throughput sequencing	653:687	16S rDNA high-throughput sequencing	653:687	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	9	59	theme	Dionex	1348:1353	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	9	60	theme	glucose	1501:1507	arg1	peaks					1444:1448	absorption peaks	1433:1448	absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose	1433:1519	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	8	61	theme	propionic	1278:1286	arg1	acid					1288:1291	propionic acid	1278:1291	propionic acid	1278:1291	EPSs also increased the production of propionic acid compared to the starch group.
30569218	9	62	theme	ICS	1355:1357	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	4	63	theme	gut	596:598	arg1	microbiota					600:609	human gut microbiota	590:609	human gut microbiota	590:609	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	6	64	theme	VI_Starch	1041:1049	arg1	group					1051:1055	the VI_Starch group	1037:1055	the VI_Starch group	1037:1055	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	3	65	from	benefits	420:427	arg1	food					432:435	food	432:435	food	432:435	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	3	65	from	benefits	420:427	arg1	health					441:446	health	441:446	health	441:446	EPS production not only has benefits in food and health but also has effects on probiotics in the microbial ecosystem.
30569218	7	66	theme	Coprococcus	1227:1237	arg1	growth					1140:1145	the growth	1136:1145	the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus	1136:1237	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	4	67	theme	human	590:594	arg1	microbiota					600:609	human gut microbiota	590:609	human gut microbiota	590:609	In this study, we investigated the interaction between bifidobacteria EPSs and human gut microbiota in vitro using thin-layer chromatography, 16S rDNA high-throughput sequencing, and gas chromatography.
30569218	7	68	theme	order	1196:1200	arg1	Deinococcales					1202:1214	order Deinococcales	1196:1214	order Deinococcales	1196:1214	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	10	69	theme	EPS	1651:1653	arg1	complex					1674:1680	complex	1674:1680	complex	1674:1680	The monosaccharide composition of this EPS appears to be more complex than previously reported for bifidobacteria EPS.
30569218	10	69	theme	EPS	1651:1653	arg1	composition					1631:1641	The monosaccharide composition	1612:1641	The monosaccharide composition of this EPS	1612:1653	The monosaccharide composition of this EPS appears to be more complex than previously reported for bifidobacteria EPS.
30569218	5	70	from	48 h.	866:870	arg1	hand					885:888	the other hand	875:888	the other hand	875:888	The results showed that human gut microbiota has the capacity to degrade EPSs, although the degradation rate was approximately 50% after fermenting for 48 h. On the other hand, EPSs regulate the human gut microbiota.
30569218	9	71	theme	high-purity	1364:1374	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	7	72	theme	Deinococcales	1202:1214	arg1	growth					1140:1145	the growth	1136:1145	the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus	1136:1237	The bifidobacteria EPS promoted the growth of phylum Deinococcus_Thermus, class Deinococci, order Deinococcales, and genus Coprococcus.
30569218	9	73	dep	3	1605:1605	arg1	53					1608:1609	53	1608:1609	53	1608:1609	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
30569218	6	74	theme	bacterial	1005:1013	arg1	community					1015:1023	the bacterial community	1001:1023	the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source	1001:1101	Fermented samples in the VI_Bif group clustered together according to the bacterial community compared to the VI_Starch group, in which starch was added as a carbon source.
30569218	9	75	theme	capillary	1376:1384	arg1	system					1405:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	Dionex ICS 5000 high-purity capillary ion chromatography system	1348:1410	The detection results of Dionex ICS 5000 high-purity capillary ion chromatography system showed that EPSs had absorption peaks of fucose, rhamnose, galactose/acetyl glucosamine, glucose, and ribose, and the molecular proportion of these monosaccharides was approximately 2: 2: 440: 3: 53.
31054300	2	0	theme	section	456:462	arg1	histopathology					464:477	tissue section histopathology	449:477	tissue section histopathology	449:477	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	7	1	theme	PSP	1348:1350	arg1	function					1336:1343	a new function	1330:1343	a new function of PSP	1330:1350	The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
31054300	2	2	dep	treated	302:308	arg1	followed					339:346	followed	339:346	followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology	339:477	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	5	3	from	renovation	885:894	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	2	4	theme	tissue	449:454	arg1	histopathology					464:477	tissue section histopathology	449:477	tissue section histopathology	449:477	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	1	5	theme	polysaccharide	158:171	arg1	functions					143:151	new functions	139:151	new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	139:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	5	6	from	expression	950:959	arg1	mice					1002:1005	the constipated mice	986:1005	the constipated mice	986:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	7	from	improvement	814:824	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	8	theme	NO	867:868	arg1	concentration					870:882	NO concentration	867:882	NO concentration	867:882	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	3	9	theme	sequencing	546:555	arg1	analysis					557:564	genome sequencing analysis	539:564	genome sequencing analysis of the 16S rDNA	539:580	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	6	10	theme	bacteria	1266:1273	arg1	abundance					1245:1253	abundance	1245:1253	abundance of harmful bacteria such as Clostridium and Dorea	1245:1303	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	5	11	theme	constipated	990:1000	arg1	mice					1002:1005	the constipated mice	986:1005	the constipated mice	986:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	12	theme	concentration	870:882	arg1	reduction					854:862	reduction	854:862	reduction of NO concentration	854:882	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	12	theme	concentration	870:882	arg1	increase					827:834	increase	827:834	increase of AchE activity	827:851	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	12	theme	concentration	870:882	arg1	improvement					814:824	defecation improvement	803:824	defecation improvement	803:824	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	12	theme	concentration	870:882	arg1	affection					933:941	affection	933:941	affection on the expression of some related genes in the constipated mice	933:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	12	theme	concentration	870:882	arg1	renovation					885:894	renovation	885:894	renovation of the damaged intestinal villus	885:927	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	1	13	from	functions	143:151	arg1	platensis					188:196	Spirulina platensis	178:196	Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	178:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	1	13	from	functions	143:151	arg1	PSP					199:201	PSP	199:201	PSP	199:201	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	5	14	theme	defecation	803:812	arg1	improvement					814:824	defecation improvement	803:824	defecation improvement	803:824	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	1	15	theme	new	139:141	arg1	functions					143:151	new functions	139:151	new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	139:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	6	16	theme	harmful	1258:1264	arg1	bacteria					1266:1273	harmful bacteria	1258:1273	harmful bacteria such as Clostridium and Dorea	1258:1303	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	16	theme	harmful	1258:1264	arg1	Clostridium					1283:1293	Clostridium	1283:1293	Clostridium	1283:1293	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	16	theme	harmful	1258:1264	arg1	Dorea					1299:1303	Dorea	1299:1303	Dorea	1299:1303	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	3	17	theme	microbiota	510:519	arg1	composition					484:494	The composition	480:494	The composition of intestinal microbiota	480:519	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	2	18	theme	acetyl	396:401	arg1	oxide					433:437	nitric oxide	426:437	nitric oxide (NO)	426:442	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	18	theme	acetyl	396:401	arg1	AchE					419:422	AchE	419:422	AchE	419:422	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	18	theme	acetyl	396:401	arg1	cholinesterase					403:416	acetyl cholinesterase	396:416	acetyl cholinesterase (AchE)	396:423	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	6	19	theme	gut	1056:1058	arg1	microbiota					1060:1069	the gut microbiota	1052:1069	the gut microbiota	1052:1069	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	1	20	theme	Spirulina	178:186	arg1	platensis					188:196	Spirulina platensis	178:196	Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	178:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	1	20	theme	Spirulina	178:186	arg1	PSP					199:201	PSP	199:201	PSP	199:201	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	3	21	theme	intestinal	499:508	arg1	microbiota					510:519	intestinal microbiota	499:519	intestinal microbiota	499:519	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	5	22	theme	activity	844:851	arg1	reduction					854:862	reduction	854:862	reduction of NO concentration	854:882	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	22	theme	activity	844:851	arg1	increase					827:834	increase	827:834	increase of AchE activity	827:851	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	22	theme	activity	844:851	arg1	improvement					814:824	defecation improvement	803:824	defecation improvement	803:824	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	22	theme	activity	844:851	arg1	affection					933:941	affection	933:941	affection on the expression of some related genes in the constipated mice	933:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	22	theme	activity	844:851	arg1	renovation					885:894	renovation	885:894	renovation of the damaged intestinal villus	885:927	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	6	23	from	reduction	1232:1240	arg1	abundance					1099:1107	abundance	1099:1107	abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella	1099:1221	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	23	from	reduction	1232:1240	arg1	abundance					1245:1253	abundance	1245:1253	abundance of harmful bacteria such as Clostridium and Dorea	1245:1303	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	5	24	theme	beneficial	759:768	arg1	effects					770:776	The beneficial effects	755:776	The beneficial effects of PSP treatment	755:793	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	25	from	affection	933:941	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	26	theme	PSP	781:783	arg1	treatment					785:793	PSP treatment	781:793	PSP treatment	781:793	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	2	27	theme	nitric	426:431	arg1	oxide					433:437	nitric oxide	426:437	nitric oxide (NO)	426:442	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	27	theme	nitric	426:431	arg1	cholinesterase					403:416	acetyl cholinesterase	396:416	acetyl cholinesterase (AchE)	396:423	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	27	theme	nitric	426:431	arg1	NO					440:441	NO	440:441	NO	440:441	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	5	28	theme	AchE	839:842	arg1	activity					844:851	AchE activity	839:851	AchE activity	839:851	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	2	29	theme	different	315:323	arg1	doses					325:329	different doses	315:329	different doses of PSP	315:336	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	5	30	theme	villus	922:927	arg1	reduction					854:862	reduction	854:862	reduction of NO concentration	854:882	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	30	theme	villus	922:927	arg1	increase					827:834	increase	827:834	increase of AchE activity	827:851	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	30	theme	villus	922:927	arg1	improvement					814:824	defecation improvement	803:824	defecation improvement	803:824	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	30	theme	villus	922:927	arg1	affection					933:941	affection	933:941	affection on the expression of some related genes in the constipated mice	933:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	30	theme	villus	922:927	arg1	renovation					885:894	renovation	885:894	renovation of the damaged intestinal villus	885:927	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	4	31	theme	average	609:615	arg1	weight					627:632	the average molecular weight	605:632	the average molecular weight of PSP	605:639	This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%).
31054300	0	32	theme	Spirulina	20:28	arg1	platensis					30:38	Spirulina platensis	20:38	Spirulina platensis	20:38	Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice.
31054300	7	33	theme	present	1310:1316	arg1	s					1318:1318	The present s	1306:1318	The present s	1306:1318	The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
31054300	5	34	theme	related	969:975	arg1	genes					977:981	some related genes	964:981	some related genes	964:981	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	1	35	theme	study	121:125	arg1	aim					109:111	The aim	105:111	The aim of this study	105:125	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	2	36	from	constipation	277:288	arg1	mice					293:296	mice	293:296	mice	293:296	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	0	37	theme	diphenoxylate-induced	52:72	arg1	symptoms					87:94	diphenoxylate-induced constipation symptoms	52:94	diphenoxylate-induced constipation symptoms	52:94	Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice.
31054300	5	38	theme	genes	977:981	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	3	39	theme	rDNA	577:580	arg1	analysis					557:564	genome sequencing analysis	539:564	genome sequencing analysis of the 16S rDNA	539:580	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	4	40	theme	PSP	637:639	arg1	weight					627:632	the average molecular weight	605:632	the average molecular weight of PSP	605:639	This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%).
31054300	6	41	theme	bacteria	1123:1130	arg1	abundance					1099:1107	abundance	1099:1107	abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella	1099:1221	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	2	42	theme	cholinesterase	403:416	arg1	histopathology					464:477	tissue section histopathology	449:477	tissue section histopathology	449:477	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	42	theme	cholinesterase	403:416	arg1	levels					386:391	levels	386:391	levels of acetyl cholinesterase (AchE), nitric oxide (NO)	386:442	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	2	42	theme	cholinesterase	403:416	arg1	patterns					376:383	the defecation patterns	361:383	the defecation patterns	361:383	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	7	43	used	used	1382:1385	arg2	PSP					1369:1371	PSP	1369:1371	PSP	1369:1371	The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
31054300	1	44	from	microbiota	235:244	arg1	mice					249:252	mice	249:252	mice	249:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	6	45	contain	had	1025:1027	arg1	PSP					1021:1023	PSP	1021:1023	PSP	1021:1023	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	45	contain	had	1025:1027	arg2	effects					1041:1047	significant effects	1029:1047	significant effects	1029:1047	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	46	theme	beneficial	1112:1121	arg1	Lactobacillus					1155:1167	Lactobacillus	1155:1167	Lactobacillus	1155:1167	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	46	theme	beneficial	1112:1121	arg1	bacteria					1123:1130	beneficial bacteria	1112:1130	beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella	1112:1221	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	46	theme	beneficial	1112:1121	arg1	Prevotella					1212:1221	Prevotella	1212:1221	Prevotella	1212:1221	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	46	theme	beneficial	1112:1121	arg1	Arthromitus					1196:1206	Arthromitus	1196:1206	Arthromitus	1196:1206	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	1	47	from	constipation	207:218	arg1	mice					249:252	mice	249:252	mice	249:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	0	48	theme	constipation	74:85	arg1	symptoms					87:94	diphenoxylate-induced constipation symptoms	52:94	diphenoxylate-induced constipation symptoms	52:94	Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice.
31054300	7	49	theme	constipation	1390:1401	arg1	therapies					1403:1411	constipation therapies	1390:1411	constipation therapies	1390:1411	The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
31054300	3	50	theme	16S	573:575	arg1	rDNA					577:580	the 16S rDNA	569:580	the 16S rDNA	569:580	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	7	51	theme	new	1332:1334	arg1	function					1336:1343	a new function	1330:1343	a new function of PSP	1330:1350	The present s uncovered a new function of PSP, indicating that PSP could be used in constipation therapies.
31054300	5	52	from	reduction	854:862	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	53	from	increase	827:834	arg1	expression					950:959	the expression	946:959	the expression of some related genes in the constipated mice	946:1005	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	4	54	theme	PSP	687:689	arg1	PSP					687:689	PSP	687:689	PSP	687:689	This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%).
31054300	4	54	theme	PSP	687:689	arg1	29					645:646	29	645:646	29	645:646	This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%).
31054300	3	55	theme	genome	539:544	arg1	analysis					557:564	genome sequencing analysis	539:564	genome sequencing analysis of the 16S rDNA	539:580	The composition of intestinal microbiota was determined by genome sequencing analysis of the 16S rDNA.
31054300	6	56	theme	significant	1029:1039	arg1	effects					1041:1047	significant effects	1029:1047	significant effects	1029:1047	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	2	57	theme	Diphenoxylate-induced	255:275	arg1	constipation					277:288	Diphenoxylate-induced constipation	255:288	Diphenoxylate-induced constipation in mice	255:296	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	1	58	from	platensis	188:196	arg1	microbiota					235:244	intestinal microbiota	224:244	intestinal microbiota	224:244	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	1	58	from	platensis	188:196	arg1	constipation					207:218	constipation	207:218	constipation	207:218	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	1	58	from	platensis	188:196	arg1	functions					143:151	new functions	139:151	new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	139:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	1	58	from	platensis	188:196	arg1	polysaccharide					158:171	a polysaccharide	156:171	a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice	156:252	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	2	59	theme	PSP	334:336	arg1	doses					325:329	different doses	315:329	different doses of PSP	315:336	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	1	60	theme	intestinal	224:233	arg1	microbiota					235:244	intestinal microbiota	224:244	intestinal microbiota	224:244	The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice.
31054300	4	61	theme	molecular	617:625	arg1	weight					627:632	the average molecular weight	605:632	the average molecular weight of PSP	605:639	This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%).
31054300	5	62	theme	damaged	903:909	arg1	villus					922:927	the damaged intestinal villus	899:927	the damaged intestinal villus	899:927	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	5	63	theme	treatment	785:793	arg1	effects					770:776	The beneficial effects	755:776	The beneficial effects of PSP treatment	755:793	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	0	64	from	platensis	30:38	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide from Spirulina platensis	0:38	Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice.
31054300	5	65	theme	intestinal	911:920	arg1	villus					922:927	the damaged intestinal villus	899:927	the damaged intestinal villus	899:927	The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice.
31054300	2	66	theme	defecation	365:374	arg1	patterns					376:383	the defecation patterns	361:383	the defecation patterns	361:383	Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE), nitric oxide (NO), and tissue section histopathology.
31054300	6	67	from	enhancement	1084:1094	arg1	abundance					1099:1107	abundance	1099:1107	abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella	1099:1221	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31054300	6	67	from	enhancement	1084:1094	arg1	abundance					1245:1253	abundance	1245:1253	abundance of harmful bacteria such as Clostridium and Dorea	1245:1303	In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and Prevotella, and the reduction in abundance of harmful bacteria such as Clostridium and Dorea.
31233046	2	0	theme	polymer	463:469	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	3	1	theme	elastic	679:685	arg1	modulus					687:693	simulated elastic modulus	669:693	simulated elastic modulus	669:693	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	2	2	theme	eyelid	587:592	arg1	lamella					576:582	posterior lamella	566:582	posterior lamella of eyelid	566:592	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	1	3	theme	corneal	160:166	arg1	irritation					168:177	corneal irritation	160:177	corneal irritation	160:177	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	1	4	theme	blepharon	257:265	arg1	deformities					267:277	blindness and cicatricial blepharon deformities	231:277	blindness and cicatricial blepharon deformities	231:277	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	3	5	theme	%	666:666	arg1	porosity					651:658	porosity	651:658	porosity of ∼90%	651:666	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	5	theme	%	666:666	arg1	rate					720:723	appropriate degradation rate	696:723	appropriate degradation rate	696:723	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	5	theme	%	666:666	arg1	biocompatibility					734:749	good biocompatibility	729:749	good biocompatibility	729:749	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	5	theme	%	666:666	arg1	modulus					687:693	simulated elastic modulus	669:693	simulated elastic modulus	669:693	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	2	6	theme	μm	352:353	arg1	μm					372:373	724 μm	368:373	724 μm	368:373	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	6	theme	μm	352:353	arg1	thickness					335:343	thickness	335:343	thickness of 240 μm	335:353	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	6	theme	μm	352:353	arg1	μm					360:361	466 μm	356:361	466 μm	356:361	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	5	7	theme	functional	1097:1106	arg1	conjunctiva					1120:1130	functional regenerated conjunctiva	1097:1130	functional regenerated conjunctiva	1097:1130	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	2	8	theme	biphasic	508:515	arg1	scaffolds					517:525	the corresponding biphasic scaffolds	490:525	the corresponding biphasic scaffolds	490:525	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	5	9	theme	tarso-conjunctival	1001:1018	arg1	model					1027:1031	a rabbit tarso-conjunctival defect model	992:1031	a rabbit tarso-conjunctival defect model	992:1031	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	2	10	theme	lamella	576:582	arg1	anatomy					555:561	the natural anatomy	543:561	the natural anatomy of posterior lamella of eyelid	543:592	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	11	theme	collagen/chitosan	295:311	arg1	sponges					322:328	collagen/chitosan (Col/CS) sponges	295:328	collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm	295:373	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	12	with	sponges	322:328	arg1	μm					372:373	724 μm	368:373	724 μm	368:373	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	12	with	sponges	322:328	arg1	thickness					335:343	thickness	335:343	thickness of 240 μm	335:353	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	12	with	sponges	322:328	arg1	μm					360:361	466 μm	356:361	466 μm	356:361	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	13	theme	corresponding	494:506	arg1	scaffolds					517:525	the corresponding biphasic scaffolds	490:525	the corresponding biphasic scaffolds	490:525	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	3	14	theme	appropriate	696:706	arg1	rate					720:723	appropriate degradation rate	696:723	appropriate degradation rate	696:723	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	2	15	theme	posterior	566:574	arg1	lamella					576:582	posterior lamella	566:582	posterior lamella of eyelid	566:592	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	5	16	with	re-epithelization	1074:1090	arg1	conjunctiva					1120:1130	functional regenerated conjunctiva	1097:1130	functional regenerated conjunctiva	1097:1130	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	4	17	theme	effective	970:978	arg1	modulus					980:986	effective modulus	970:986	effective modulus	970:986	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	3	18	theme	degradation	708:718	arg1	rate					720:723	appropriate degradation rate	696:723	appropriate degradation rate	696:723	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	4	19	theme	cellular	839:846	arg1	behaviors					848:856	different cellular behaviors	829:856	different cellular behaviors such as proliferation, distribution and stratification	829:911	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	19	theme	cellular	839:846	arg1	proliferation					866:878	proliferation	866:878	proliferation	866:878	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	19	theme	cellular	839:846	arg1	stratification					898:911	stratification	898:911	stratification	898:911	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	19	theme	cellular	839:846	arg1	distribution					881:892	distribution	881:892	distribution	881:892	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	20	theme	Col/CS	768:773	arg1	sponge					775:780	Col/CS sponge	768:780	Col/CS sponge of difference thickness	768:804	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	6	21	dep	promising	1182:1190	arg1	substitute					1192:1201	substitute	1192:1201	substitute for tarso-conjunctival repair	1192:1231	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	21	dep	promising	1182:1190	arg1	scaffolds					1163:1171	the biphasic composite scaffolds	1140:1171	the biphasic composite scaffolds	1140:1171	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	21	dep	promising	1182:1190	arg1	promising					1182:1190	promising	1182:1190	promising	1182:1190	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	2	22	theme	-co-2-hydroxyethyl	420:437	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	4	23	theme	different	829:837	arg1	behaviors					848:856	different cellular behaviors	829:856	different cellular behaviors such as proliferation, distribution and stratification	829:911	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	23	theme	different	829:837	arg1	proliferation					866:878	proliferation	866:878	proliferation	866:878	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	23	theme	different	829:837	arg1	stratification					898:911	stratification	898:911	stratification	898:911	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	4	23	theme	different	829:837	arg1	distribution					881:892	distribution	881:892	distribution	881:892	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	1	24	theme	Conventional	85:96	arg1	scaffolds					108:116	Conventional 3D porous scaffolds	85:116	Conventional 3D porous scaffolds used as tarsal plate substitute	85:148	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	1	25	theme	conjunctival	183:194	arg1	discharge					203:211	conjunctival mucoid discharge	183:211	conjunctival mucoid discharge	183:211	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	0	26	theme	biomimetic	2:11	arg1	scaffold					41:48	A biomimetic tarso-conjunctival biphasic scaffold	0:48	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo	0:82	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo.
31233046	1	27	theme	3D	98:99	arg1	scaffolds					108:116	Conventional 3D porous scaffolds	85:116	Conventional 3D porous scaffolds used as tarsal plate substitute	85:148	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	1	28	theme	mucoid	196:201	arg1	discharge					203:211	conjunctival mucoid discharge	183:211	conjunctival mucoid discharge	183:211	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	2	29	theme	fumarate	411:418	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	3	30	theme	good	729:732	arg1	biocompatibility					734:749	good biocompatibility	729:749	good biocompatibility	729:749	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	1	31	theme	porous	101:106	arg1	scaffolds					108:116	Conventional 3D porous scaffolds	85:116	Conventional 3D porous scaffolds used as tarsal plate substitute	85:148	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	0	32	theme	biphasic	32:39	arg1	scaffold					41:48	A biomimetic tarso-conjunctival biphasic scaffold	0:48	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo	0:82	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo.
31233046	5	33	theme	defect	1020:1025	arg1	model					1027:1031	a rabbit tarso-conjunctival defect model	992:1031	a rabbit tarso-conjunctival defect model	992:1031	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	2	34	theme	Col/CS	314:319	arg1	sponges					322:328	collagen/chitosan (Col/CS) sponges	295:328	collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm	295:373	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	35	theme	poly	396:399	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	0	36	theme	tarso-conjunctival	13:30	arg1	scaffold					41:48	A biomimetic tarso-conjunctival biphasic scaffold	0:48	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo	0:82	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo.
31233046	3	37	theme	porous	629:634	arg1	structure					636:644	a porous structure	627:644	a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility	627:749	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	2	38	theme	PPF-HEMA	453:460	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	4	39	theme	properties	943:952	arg1	cells					954:958	the mechanical properties cells	928:958	the mechanical properties cells sensed as effective modulus	928:986	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	5	40	theme	regenerated	1108:1118	arg1	conjunctiva					1120:1130	functional regenerated conjunctiva	1097:1130	functional regenerated conjunctiva	1097:1130	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	3	41	with	structure	636:644	arg1	porosity					651:658	porosity	651:658	porosity of ∼90%	651:666	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	41	with	structure	636:644	arg1	rate					720:723	appropriate degradation rate	696:723	appropriate degradation rate	696:723	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	41	with	structure	636:644	arg1	biocompatibility					734:749	good biocompatibility	729:749	good biocompatibility	729:749	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	3	41	with	structure	636:644	arg1	modulus					687:693	simulated elastic modulus	669:693	simulated elastic modulus	669:693	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	4	42	theme	mechanical	932:941	arg1	properties					943:952	the mechanical properties	928:952	the mechanical properties cells sensed as effective modulus	928:986	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	2	43	theme	propylene	401:409	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	2	44	theme	methacrylate	439:450	arg1	networks					471:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks	396:478	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	5	45	theme	grafted	1038:1044	arg1	scaffolds					1055:1063	the grafted biphasic scaffolds	1034:1063	the grafted biphasic scaffolds	1034:1063	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	0	46	theme	eyelid	54:59	arg1	reconstruction					61:74	eyelid reconstruction	54:74	eyelid reconstruction in vivo	54:82	A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo.
31233046	5	47	theme	biphasic	1046:1053	arg1	scaffolds					1055:1063	the grafted biphasic scaffolds	1034:1063	the grafted biphasic scaffolds	1034:1063	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	1	48	theme	tarsal	126:131	arg1	plate					133:137	tarsal plate substitute	126:148	tarsal plate substitute	126:148	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	2	49	theme	natural	547:553	arg1	anatomy					555:561	the natural anatomy	543:561	the natural anatomy of posterior lamella of eyelid	543:592	In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)-co-2-hydroxyethyl methacrylate (PPF-HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid.
31233046	5	50	theme	rabbit	994:999	arg1	model					1027:1031	a rabbit tarso-conjunctival defect model	992:1031	a rabbit tarso-conjunctival defect model	992:1031	In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva.
31233046	6	51	theme	tarso-conjunctival	1207:1224	arg1	repair					1226:1231	tarso-conjunctival repair	1207:1231	tarso-conjunctival repair	1207:1231	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	1	52	dep	plate	133:137	arg1	substitute					139:148	substitute	139:148	substitute	139:148	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	6	53	theme	composite	1153:1161	arg1	substitute					1192:1201	substitute	1192:1201	substitute for tarso-conjunctival repair	1192:1231	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	53	theme	composite	1153:1161	arg1	scaffolds					1163:1171	the biphasic composite scaffolds	1140:1171	the biphasic composite scaffolds	1140:1171	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	53	theme	composite	1153:1161	arg1	promising					1182:1190	promising	1182:1190	promising	1182:1190	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	1	54	theme	blindness	231:239	arg1	deformities					267:277	blindness and cicatricial blepharon deformities	231:277	blindness and cicatricial blepharon deformities	231:277	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
31233046	4	55	theme	thickness	796:804	arg1	sponge					775:780	Col/CS sponge	768:780	Col/CS sponge of difference thickness	768:804	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	6	56	theme	biphasic	1144:1151	arg1	substitute					1192:1201	substitute	1192:1201	substitute for tarso-conjunctival repair	1192:1231	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	56	theme	biphasic	1144:1151	arg1	scaffolds					1163:1171	the biphasic composite scaffolds	1140:1171	the biphasic composite scaffolds	1140:1171	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	6	56	theme	biphasic	1144:1151	arg1	promising					1182:1190	promising	1182:1190	promising	1182:1190	Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.
31233046	3	57	theme	simulated	669:677	arg1	modulus					687:693	simulated elastic modulus	669:693	simulated elastic modulus	669:693	These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility.
31233046	4	58	theme	difference	785:794	arg1	thickness					796:804	difference thickness	785:804	difference thickness	785:804	Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus.
31233046	1	59	theme	cicatricial	245:255	arg1	deformities					267:277	blindness and cicatricial blepharon deformities	231:277	blindness and cicatricial blepharon deformities	231:277	Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities.
29779131	0	0	theme	toebii	112:117	arg1	strains					126:132	Geobacillus toebii ArzA-8 strains	100:132	Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring	100:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	2	1	theme	sequence	509:516	arg1	analysis					518:525	16S rRNA gene sequence analysis	495:525	16S rRNA gene sequence analysis	495:525	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	2	theme	thermodenitrificans	589:607	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	2	theme	thermodenitrificans	589:607	arg1	ArzA-6					609:614	Geobacillus thermodenitrificans ArzA-6	577:614	Geobacillus thermodenitrificans ArzA-6	577:614	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	1	3	theme	thermal	183:189	arg1	springs					224:230	geothermal springs	213:230	geothermal springs	213:230	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	1	3	theme	thermal	183:189	arg1	source					251:256	source	251:256	source of thermophiles able to produce extracellular polysaccharides (EPSs)	251:325	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	1	3	theme	thermal	183:189	arg1	ecosystems					191:200	The thermal ecosystems	179:200	The thermal ecosystems	179:200	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	5	4	theme	G.	1124:1125	arg1	ArzA-8					1134:1139	G. toebii ArzA-8	1124:1139	G. toebii ArzA-8	1124:1139	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	1	5	theme	able	274:277	arg1	thermophiles					261:272	thermophiles	261:272	thermophiles able to produce extracellular polysaccharides (EPSs)	261:325	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	0	6	theme	Geobacillus	100:110	arg1	strains					126:132	Geobacillus toebii ArzA-8 strains	100:132	Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring	100:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	4	7	used	used	965:968	arg2	source					985:990	sole carbon source	973:990	sole carbon source	973:990	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	7	used	used	965:968	arg2	fructose					952:959	fructose	952:959	fructose	952:959	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	8	theme	0.22 g g-1	824:833	arg1	cells					839:843	0.22 g g-1 dry cells	824:843	0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii	824:899	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	0	9	theme	ArzA-8	119:124	arg1	strains					126:132	Geobacillus toebii ArzA-8 strains	100:132	Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring	100:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	2	10	attach	isolated	367:374	arg1	sediment					381:388	sediment	381:388	sediment sampled from Arzakan geothermal spring	381:427	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	10	attach	isolated	367:374	arg2	bacilli					359:365	the sixteen thermophilic bacilli	334:365	the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring	334:427	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	11	theme	ArzA-8	639:644	arg1	strains					646:652	Geobacillus toebii ArzA-8 strains	620:652	Geobacillus toebii ArzA-8 strains	620:652	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	11	theme	ArzA-8	639:644	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	4	12	theme	highest	759:765	arg1	yield					792:796	The highest specific EPSs production yield	755:796	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively)	755:921	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	1	13	theme	extracellular	290:302	arg1	EPSs					321:324	EPSs	321:324	EPSs	321:324	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	1	13	theme	extracellular	290:302	arg1	polysaccharides					304:318	extracellular polysaccharides	290:318	extracellular polysaccharides (EPSs)	290:325	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	5	14	theme	toebii	1127:1132	arg1	ArzA-8					1134:1139	G. toebii ArzA-8	1124:1139	G. toebii ArzA-8	1124:1139	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	7	15	theme	2 mg ml-1	1380:1388	arg1	EPSs					1374:1377	the two EPSs	1366:1377	the two EPSs (2 mg ml-1 H2O)	1366:1393	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	7	15	theme	2 mg ml-1	1380:1388	arg1	H2O					1390:1392	2 mg ml-1 H2O	1380:1392	2 mg ml-1 H2O	1380:1392	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	4	16	dep	G.	891:892	arg1	toebii					894:899	G. toebii	891:899	G. toebii	891:899	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	17	theme	cells	839:843	arg1	ArzA-8					901:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	18	dep	strains	849:855	arg1	thermodenitrificans					860:878	thermodenitrificans	860:878	thermodenitrificans	860:878	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	18	dep	strains	849:855	arg1	G.					891:892	G.	891:892	G.	891:892	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	18	dep	strains	849:855	arg1	strains					849:855	strains G. thermodenitrificans ArzA-6 and G. toebii	849:899	strains G. thermodenitrificans ArzA-6 and G. toebii	849:899	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	2	19	theme	Armenia	430:436	arg1	EPSs					448:451	Armenia, two best EPSs	430:451	EPSs	448:451	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	20	theme	phenotypic	531:540	arg1	characteristics					542:556	phenotypic characteristics	531:556	phenotypic characteristics	531:556	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	4	21	theme	dry	835:837	arg1	cells					839:843	0.22 g g-1 dry cells	824:843	0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii	824:899	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	2	22	theme	gene	504:507	arg1	analysis					518:525	16S rRNA gene sequence analysis	495:525	16S rRNA gene sequence analysis	495:525	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	4	23	theme	sole	973:976	arg1	fructose					952:959	fructose	952:959	fructose	952:959	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	23	theme	sole	973:976	arg1	source					985:990	sole carbon source	973:990	sole carbon source	973:990	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	24	theme	0.27 g g-1	799:808	arg1	cells					814:818	0.27 g g-1 dry cells	799:818	0.27 g g-1 dry cells	799:818	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	1	25	theme	geothermal	213:222	arg1	springs					224:230	geothermal springs	213:230	geothermal springs	213:230	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	4	26	theme	production	781:790	arg1	yield					792:796	The highest specific EPSs production yield	755:796	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively)	755:921	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	6	27	theme	biopolymers	1184:1194	arg1	composition					1151:1161	Chemical composition	1142:1161	Chemical composition	1142:1161	Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit.
29779131	6	27	theme	biopolymers	1184:1194	arg1	structure					1167:1175	structure	1167:1175	structure	1167:1175	Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit.
29779131	2	28	theme	geothermal	411:420	arg1	spring					422:427	Arzakan geothermal spring	403:427	Arzakan geothermal spring	403:427	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	0	29	theme	Armenian	151:158	arg1	spring					171:176	an Armenian geothermal spring	148:176	an Armenian geothermal spring	148:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	4	30	theme	cells	814:818	arg1	ArzA-8					901:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	2	31	theme	EPSs	448:451	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	31	theme	EPSs	448:451	arg1	ArzA-6					609:614	Geobacillus thermodenitrificans ArzA-6	577:614	Geobacillus thermodenitrificans ArzA-6	577:614	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	31	theme	EPSs	448:451	arg1	strains					646:652	Geobacillus toebii ArzA-8 strains	620:652	Geobacillus toebii ArzA-8 strains	620:652	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	32	theme	producer	453:460	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	32	theme	producer	453:460	arg1	ArzA-6					609:614	Geobacillus thermodenitrificans ArzA-6	577:614	Geobacillus thermodenitrificans ArzA-6	577:614	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	32	theme	producer	453:460	arg1	strains					646:652	Geobacillus toebii ArzA-8 strains	620:652	Geobacillus toebii ArzA-8 strains	620:652	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	33	theme	Geobacillus	577:587	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	33	theme	Geobacillus	577:587	arg1	ArzA-6					609:614	Geobacillus thermodenitrificans ArzA-6	577:614	Geobacillus thermodenitrificans ArzA-6	577:614	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	4	34	theme	carbon	978:983	arg1	fructose					952:959	fructose	952:959	fructose	952:959	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	34	theme	carbon	978:983	arg1	source					985:990	sole carbon source	973:990	sole carbon source	973:990	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	4	35	theme	dry	810:812	arg1	cells					814:818	0.27 g g-1 dry cells	799:818	0.27 g g-1 dry cells	799:818	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	2	36	theme	best	443:446	arg1	EPSs					448:451	Armenia, two best EPSs	430:451	EPSs	448:451	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	37	theme	rRNA	499:502	arg1	analysis					518:525	16S rRNA gene sequence analysis	495:525	16S rRNA gene sequence analysis	495:525	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	7	38	theme	rotation	1336:1343	arg1	values					1345:1350	Optical rotation values	1328:1350	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O)	1328:1393	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	2	39	theme	16S	495:497	arg1	analysis					518:525	16S rRNA gene sequence analysis	495:525	16S rRNA gene sequence analysis	495:525	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	0	40	theme	exopolysaccharides	35:52	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	0	40	theme	exopolysaccharides	35:52	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	3	41	theme	different	694:702	arg1	composition					742:752	different time, temperature and culture media's composition	694:752	different time, temperature and culture media's composition	694:752	EPSs production was investigated under different time, temperature and culture media's composition.
29779131	3	42	theme	EPSs	655:658	arg1	production					660:669	EPSs production	655:669	EPSs production	655:669	EPSs production was investigated under different time, temperature and culture media's composition.
29779131	2	43	theme	toebii	632:637	arg1	strains					646:652	Geobacillus toebii ArzA-8 strains	620:652	Geobacillus toebii ArzA-8 strains	620:652	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	43	theme	toebii	632:637	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	7	44	dep	values	1345:1350	arg1	D25 °C					1356:1361	D25 °C	1356:1361	D25 °C	1356:1361	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	4	45	theme	EPSs	776:779	arg1	yield					792:796	The highest specific EPSs production yield	755:796	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively)	755:921	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	0	46	theme	geothermal	160:169	arg1	spring					171:176	an Armenian geothermal spring	148:176	an Armenian geothermal spring	148:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	2	47	theme	Geobacillus	620:630	arg1	strains					646:652	Geobacillus toebii ArzA-8 strains	620:652	Geobacillus toebii ArzA-8 strains	620:652	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	2	47	theme	Geobacillus	620:630	arg1	strains					462:468	Armenia, two best EPSs producer strains	430:468	Armenia, two best EPSs producer strains	430:468	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	4	48	theme	specific	767:774	arg1	EPSs					776:779	specific EPSs	767:779	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively)	755:921	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	6	49	theme	monomer	1314:1320	arg1	unit					1322:1325	major monomer unit	1308:1325	major monomer unit	1308:1325	Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit.
29779131	5	50	theme	G.	1075:1076	arg1	ArzA-6					1098:1103	G. thermodenitrificans ArzA-6	1075:1103	G. thermodenitrificans ArzA-6	1075:1103	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	4	51	dep	yield	792:796	arg1	ArzA-8					901:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8	799:906	The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0.
29779131	6	52	theme	Chemical	1142:1149	arg1	composition					1151:1161	Chemical composition	1142:1161	Chemical composition	1142:1161	Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit.
29779131	6	53	theme	major	1308:1312	arg1	unit					1322:1325	major monomer unit	1308:1325	major monomer unit	1308:1325	Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit.
29779131	5	54	theme	thermodenitrificans	1078:1096	arg1	ArzA-6					1098:1103	G. thermodenitrificans ArzA-6	1075:1103	G. thermodenitrificans ArzA-6	1075:1103	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	5	55	theme	Purified	1013:1020	arg1	EPSs					1022:1025	Purified EPSs	1013:1025	Purified EPSs	1013:1025	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	7	56	dep	D25 °C	1356:1361	arg1	[α					1352:1353	[α	1352:1353	[α	1352:1353	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	2	57	theme	thermophilic	346:357	arg1	bacilli					359:365	the sixteen thermophilic bacilli	334:365	the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring	334:427	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	7	58	theme	Optical	1328:1334	arg1	values					1345:1350	Optical rotation values	1328:1350	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O)	1328:1393	Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 for G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively.
29779131	3	59	theme	culture	726:732	arg1	media					734:738	culture media	726:738	culture media	726:738	EPSs production was investigated under different time, temperature and culture media's composition.
29779131	5	60	theme	high	1039:1042	arg1	mass					1054:1057	a high molecular mass	1037:1057	a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8	1037:1139	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	2	61	theme	Arzakan	403:409	arg1	spring					422:427	Arzakan geothermal spring	403:427	Arzakan geothermal spring	403:427	Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains.
29779131	5	62	dep	mass	1054:1057	arg1	Da					1117:1118	6 × 105 Da	1109:1118	6 × 105 Da for G. toebii ArzA-8	1109:1139	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	5	62	dep	mass	1054:1057	arg1	Da					1068:1069	5 × 105 Da	1060:1069	5 × 105 Da for G. thermodenitrificans ArzA-6	1060:1103	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	5	63	theme	molecular	1044:1052	arg1	mass					1054:1057	a high molecular mass	1037:1057	a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8	1037:1139	Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8.
29779131	0	64	attach	isolated	134:141	arg2	strains					126:132	Geobacillus toebii ArzA-8 strains	100:132	Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring	100:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	0	64	attach	isolated	134:141	arg1	spring					171:176	an Armenian geothermal spring	148:176	an Armenian geothermal spring	148:176	Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring.
29779131	1	65	theme	thermophiles	261:272	arg1	springs					224:230	geothermal springs	213:230	geothermal springs	213:230	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	1	65	theme	thermophiles	261:272	arg1	source					251:256	source	251:256	source of thermophiles able to produce extracellular polysaccharides (EPSs)	251:325	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
29779131	1	65	theme	thermophiles	261:272	arg1	ecosystems					191:200	The thermal ecosystems	179:200	The thermal ecosystems	179:200	The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs).
30110602	2	0	theme	regenerated	460:470	arg1	cellulose-Ca					472:483	pure oxidized regenerated cellulose-Ca	446:483	pure oxidized regenerated cellulose-Ca (ORCCa)	446:491	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	0	theme	regenerated	460:470	arg1	ORCCa					486:490	ORCCa	486:490	ORCCa	486:490	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	8	1	theme	composite	1427:1435	arg1	244.67 ± 13.02					1447:1460	244.67 ± 13.02	1447:1460	244.67 ± 13.02	1447:1460	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	8	1	theme	composite	1427:1435	arg1	scaffold					1437:1444	the composite scaffold	1423:1444	the composite scaffold (244.67 ± 13.02)	1423:1461	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	9	2	theme	N-Cadheri	1554:1562	arg1	superior					1642:1649	superior	1642:1649	superior	1642:1649	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	9	2	theme	N-Cadheri	1554:1562	arg1	expression					1540:1549	the expression	1536:1549	the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold	1536:1623	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	6	3	theme	proliferation	1037:1049	arg1	assays					1087:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	2	4	theme	oxidized	451:458	arg1	cellulose-Ca					472:483	pure oxidized regenerated cellulose-Ca	446:483	pure oxidized regenerated cellulose-Ca (ORCCa)	446:491	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	4	theme	oxidized	451:458	arg1	ORCCa					486:490	ORCCa	486:490	ORCCa	486:490	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	3	5	theme	tissue	647:652	arg1	composites					666:675	SC-scaffold nerve tissue engineering composites	629:675	SC-scaffold nerve tissue engineering composites	629:675	SC is then seeded on the scaffolds to form SC-scaffold nerve tissue engineering composites and evaluate their biocompatibility.
30110602	1	6	theme	primary	169:175	arg1	part					203:206	the primary structural and functional part	165:206	the primary structural and functional part of the peripheral nervous system	165:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	6	theme	primary	169:175	arg1	cell					152:155	Schwann cell	144:155	Schwann cell (SC)	144:160	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	0	7	theme	Schwann	129:135	arg1	cells					137:141	Schwann cells	129:141	Schwann cells	129:141	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	3	8	theme	engineering	654:664	arg1	composites					666:675	SC-scaffold nerve tissue engineering composites	629:675	SC-scaffold nerve tissue engineering composites	629:675	SC is then seeded on the scaffolds to form SC-scaffold nerve tissue engineering composites and evaluate their biocompatibility.
30110602	9	9	theme	PMP22	1568:1572	arg1	proteins					1574:1581	PMP22 proteins	1568:1581	PMP22 proteins	1568:1581	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	1	10	theme	key	257:259	arg1	role					261:264	a key role	255:264	a key role	255:264	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	7	11	theme	other	1359:1363	arg1	p < 0.05					1376:1383	p < 0.05	1376:1383	p < 0.05	1376:1383	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	7	11	theme	other	1359:1363	arg1	scaffolds					1365:1373	the other scaffolds	1355:1373	the other scaffolds (p < 0.05)	1355:1384	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	9	12	theme	proteins	1574:1581	arg1	superior					1642:1649	superior	1642:1649	superior	1642:1649	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	9	12	theme	proteins	1574:1581	arg1	expression					1540:1549	the expression	1536:1549	the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold	1536:1623	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	10	13	dep	repair	1911:1916	arg1	the					1907:1909	the	1907:1909	the	1907:1909	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	10	14	contain	carrying	1806:1813	arg1	scaffold					1793:1800	a scaffold	1791:1800	a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1791:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	10	14	contain	carrying	1806:1813	arg2	SC					1815:1816	SC	1815:1816	SC	1815:1816	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	9	15	from	expression	1540:1549	arg1	scaffold					1616:1623	the collagen/ORC-Ca composite scaffold	1586:1623	the collagen/ORC-Ca composite scaffold	1586:1623	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	6	16	theme	blotting	1078:1085	arg1	assays					1087:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	4	17	theme	scaffolds	757:765	arg1	structure					740:748	The chemical and physical structure	714:748	The chemical and physical structure of the scaffolds	714:765	The chemical and physical structure of the scaffolds are investigated by FTIR, NMR and SEM.
30110602	2	18	theme	SC	362:363	arg1	engineering					378:388	SC nerve tissue engineering	362:388	SC nerve tissue engineering	362:388	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	1	19	theme	structural	177:186	arg1	part					203:206	the primary structural and functional part	165:206	the primary structural and functional part of the peripheral nervous system	165:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	19	theme	structural	177:186	arg1	cell					152:155	Schwann cell	144:155	Schwann cell (SC)	144:160	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	2	20	theme	pristine	427:434	arg1	collagen					436:443	pristine collagen	427:443	pristine collagen	427:443	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	10	21	theme	tissue	1832:1837	arg1	composites					1851:1860	nerve tissue engineering composites	1826:1860	nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1826:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	5	22	theme	scaffold	945:952	arg1	strength					919:926	the tensile strength	907:926	the tensile strength of the composite scaffold (0.58 MPa)	907:963	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	22	theme	scaffold	945:952	arg1	better					968:973	better	968:973	better	968:973	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	2	23	theme	nerve	365:369	arg1	engineering					378:388	SC nerve tissue engineering	362:388	SC nerve tissue engineering	362:388	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	10	24	theme	nerve	1826:1830	arg1	composites					1851:1860	nerve tissue engineering composites	1826:1860	nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1826:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	9	25	theme	collagen/ORC-Ca	1590:1604	arg1	scaffold					1616:1623	the collagen/ORC-Ca composite scaffold	1586:1623	the collagen/ORC-Ca composite scaffold	1586:1623	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	4	26	theme	chemical	718:725	arg1	structure					740:748	The chemical and physical structure	714:748	The chemical and physical structure of the scaffolds	714:765	The chemical and physical structure of the scaffolds are investigated by FTIR, NMR and SEM.
30110602	10	27	theme	engineering	1839:1849	arg1	composites					1851:1860	nerve tissue engineering composites	1826:1860	nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1826:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	1	28	theme	functional	192:201	arg1	part					203:206	the primary structural and functional part	165:206	the primary structural and functional part of the peripheral nervous system	165:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	28	theme	functional	192:201	arg1	cell					152:155	Schwann cell	144:155	Schwann cell (SC)	144:160	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	8	29	from	number	1391:1396	arg1	244.67 ± 13.02					1447:1460	244.67 ± 13.02	1447:1460	244.67 ± 13.02	1447:1460	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	8	29	from	number	1391:1396	arg1	scaffold					1437:1444	the composite scaffold	1423:1444	the composite scaffold (244.67 ± 13.02)	1423:1461	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	9	30	theme	composite	1606:1614	arg1	scaffold					1616:1623	the collagen/ORC-Ca composite scaffold	1586:1623	the collagen/ORC-Ca composite scaffold	1586:1623	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	5	31	theme	pristine	988:995	arg1	0.36 MPa					1007:1014	0.36 MPa	1007:1014	0.36 MPa	1007:1014	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	31	theme	pristine	988:995	arg1	collagen					997:1004	pristine collagen	988:1004	pristine collagen (0.36 MPa)	988:1015	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	32	theme	scaffold	855:862	arg1	wettability					810:820	The wettability	806:820	The wettability of the collagen/ORC-Ca composite scaffold	806:862	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	32	theme	scaffold	855:862	arg1	that					876:879	that	876:879	that	876:879	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	2	33	theme	scaffolds	406:414	arg1	cellulose-Ca					472:483	pure oxidized regenerated cellulose-Ca	446:483	pure oxidized regenerated cellulose-Ca (ORCCa)	446:491	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	33	theme	scaffolds	406:414	arg1	collagen					436:443	pristine collagen	427:443	pristine collagen	427:443	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	33	theme	scaffolds	406:414	arg1	scaffolds					523:531	collagen/ORC-Ca composite scaffolds	497:531	collagen/ORC-Ca composite scaffolds	497:531	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	33	theme	scaffolds	406:414	arg1	kinds					397:401	three kinds	391:401	three kinds	391:401	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	6	34	theme	Cytotoxicity	1018:1029	arg1	assays					1087:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	4	35	theme	physical	731:738	arg1	structure					740:748	The chemical and physical structure	714:748	The chemical and physical structure of the scaffolds	714:765	The chemical and physical structure of the scaffolds are investigated by FTIR, NMR and SEM.
30110602	7	36	theme	proliferation	1251:1263	arg1	higher					1335:1340	higher	1335:1340	higher	1335:1340	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	7	36	theme	proliferation	1251:1263	arg1	rate					1265:1268	the proliferation rate	1247:1268	the proliferation rate of SC on the collagen/ORC-Ca composite scaffold	1247:1316	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	5	37	theme	composite	935:943	arg1	0.58 MPa					955:962	0.58 MPa	955:962	0.58 MPa	955:962	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	37	theme	composite	935:943	arg1	scaffold					945:952	the composite scaffold	931:952	the composite scaffold (0.58 MPa)	931:963	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	6	38	theme	cell	1032:1035	arg1	proliferation					1037:1049	cell proliferation	1032:1049	cell proliferation	1032:1049	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	2	39	theme	pure	446:449	arg1	cellulose-Ca					472:483	pure oxidized regenerated cellulose-Ca	446:483	pure oxidized regenerated cellulose-Ca (ORCCa)	446:491	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	2	39	theme	pure	446:449	arg1	ORCCa					486:490	ORCCa	486:490	ORCCa	486:490	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	6	40	theme	western	1070:1076	arg1	blotting					1078:1085	western blotting	1070:1085	western blotting	1070:1085	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	10	41	theme	promising	1768:1776	arg1	candidate					1778:1786	a promising candidate	1766:1786	a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1766:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	10	41	theme	promising	1768:1776	arg1	composite					1753:1761	the collagen/ORC-Ca composite	1733:1761	the collagen/ORC-Ca composite	1733:1761	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	7	42	theme	SC	1273:1274	arg1	higher					1335:1340	higher	1335:1340	higher	1335:1340	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	7	42	theme	SC	1273:1274	arg1	rate					1265:1268	the proliferation rate	1247:1268	the proliferation rate of SC on the collagen/ORC-Ca composite scaffold	1247:1316	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	1	43	theme	peripheral	215:224	arg1	system					234:239	the peripheral nervous system	211:239	the peripheral nervous system	211:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	2	44	theme	tissue	371:376	arg1	engineering					378:388	SC nerve tissue engineering	362:388	SC nerve tissue engineering	362:388	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	8	45	theme	ORC-Ca	1498:1503	arg1	p < 0.01					1512:1519	p < 0.01	1512:1519	p < 0.01	1512:1519	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	8	45	theme	ORC-Ca	1498:1503	arg1	group					1505:1509	the pure ORC-Ca group	1489:1509	the pure ORC-Ca group (p < 0.01)	1489:1520	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	2	46	theme	composite	513:521	arg1	scaffolds					523:531	collagen/ORC-Ca composite scaffolds	497:531	collagen/ORC-Ca composite scaffolds	497:531	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	5	47	theme	composite	845:853	arg1	scaffold					855:862	the collagen/ORC-Ca composite scaffold	825:862	the collagen/ORC-Ca composite scaffold	825:862	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	1	48	theme	nervous	226:232	arg1	system					234:239	the peripheral nervous system	211:239	the peripheral nervous system	211:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	8	49	theme	cells	1414:1418	arg1	number					1391:1396	The number	1387:1396	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02)	1387:1461	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	8	49	theme	cells	1414:1418	arg1	more					1471:1474	more	1471:1474	more	1471:1474	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	2	50	theme	collagen/ORC-Ca	497:511	arg1	scaffolds					523:531	collagen/ORC-Ca composite scaffolds	497:531	collagen/ORC-Ca composite scaffolds	497:531	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	9	51	dep	p < 0.01	1684:1691	arg1	both					1679:1682	both	1679:1682	both	1679:1682	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	10	52	theme	collagen/ORC-Ca	1737:1751	arg1	candidate					1778:1786	a promising candidate	1766:1786	a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration	1766:1933	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	10	52	theme	collagen/ORC-Ca	1737:1751	arg1	composite					1753:1761	the collagen/ORC-Ca composite	1733:1761	the collagen/ORC-Ca composite	1733:1761	Therefore, it could be concluded that the collagen/ORC-Ca composite is a promising candidate as a scaffold for carrying SC to form nerve tissue engineering composites in order to assist the peripheral nervous in the repair and regeneration.
30110602	1	53	theme	Schwann	144:150	arg1	SC					158:159	SC	158:159	SC	158:159	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	53	theme	Schwann	144:150	arg1	cell					152:155	Schwann cell	144:155	Schwann cell (SC)	144:160	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	53	theme	Schwann	144:150	arg1	part					203:206	the primary structural and functional part	165:206	the primary structural and functional part of the peripheral nervous system	165:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	0	54	theme	collagen/oxidized	54:70	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	0	54	theme	collagen/oxidized	54:70	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	0	54	theme	collagen/oxidized	54:70	arg1	biocompatibility					34:49	biocompatibility	34:49	biocompatibility	34:49	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	1	55	theme	peripheral	300:309	arg1	nerve					311:315	peripheral nerve	300:315	peripheral nerve	300:315	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	3	56	theme	SC-scaffold	629:639	arg1	composites					666:675	SC-scaffold nerve tissue engineering composites	629:675	SC-scaffold nerve tissue engineering composites	629:675	SC is then seeded on the scaffolds to form SC-scaffold nerve tissue engineering composites and evaluate their biocompatibility.
30110602	8	57	theme	adhesion	1405:1412	arg1	cells					1414:1418	the adhesion cells	1401:1418	the adhesion cells	1401:1418	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	0	58	contain	carrying	120:127	arg1	scaffold					107:114	cellulose-Ca composite scaffold	84:114	cellulose-Ca composite scaffold for carrying Schwann cells	84:141	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	0	58	contain	carrying	120:127	arg2	cells					137:141	Schwann cells	129:141	Schwann cells	129:141	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	5	59	theme	pristine	884:891	arg1	collagen					893:900	pristine collagen	884:900	pristine collagen	884:900	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	1	60	theme	nerve	311:315	arg1	repair					273:278	repair	273:278	repair	273:278	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	60	theme	nerve	311:315	arg1	regeneration					284:295	regeneration	284:295	regeneration	284:295	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	9	61	theme	other	1658:1662	arg1	scaffolds					1668:1676	the other two scaffolds	1654:1676	the other two scaffolds (both p < 0.01)	1654:1692	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	9	61	theme	other	1658:1662	arg1	p < 0.01					1684:1691	p < 0.01	1684:1691	p < 0.01	1684:1691	Furthermore, the expression of N-Cadheri and PMP22 proteins in the collagen/ORC-Ca composite scaffold is significantly superior to the other two scaffolds (both p < 0.01).
30110602	3	62	theme	nerve	641:645	arg1	composites					666:675	SC-scaffold nerve tissue engineering composites	629:675	SC-scaffold nerve tissue engineering composites	629:675	SC is then seeded on the scaffolds to form SC-scaffold nerve tissue engineering composites and evaluate their biocompatibility.
30110602	6	63	theme	scaffolds	1169:1177	arg1	properties					1145:1154	properties	1145:1154	properties	1145:1154	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	6	63	theme	scaffolds	1169:1177	arg1	biocompatibility					1124:1139	biocompatibility	1124:1139	biocompatibility	1124:1139	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	7	64	from	rate	1265:1268	arg1	scaffold					1309:1316	the collagen/ORC-Ca composite scaffold	1279:1316	the collagen/ORC-Ca composite scaffold	1279:1316	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	5	65	theme	tensile	911:917	arg1	strength					919:926	the tensile strength	907:926	the tensile strength of the composite scaffold (0.58 MPa)	907:963	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	5	65	theme	tensile	911:917	arg1	better					968:973	better	968:973	better	968:973	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	1	66	theme	system	234:239	arg1	part					203:206	the primary structural and functional part	165:206	the primary structural and functional part of the peripheral nervous system	165:239	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	1	66	theme	system	234:239	arg1	cell					152:155	Schwann cell	144:155	Schwann cell (SC)	144:160	Schwann cell (SC) is the primary structural and functional part of the peripheral nervous system, and it plays a key role in the repair and regeneration of peripheral nerve.
30110602	6	67	theme	different	1159:1167	arg1	scaffolds					1169:1177	different scaffolds	1159:1177	different scaffolds	1159:1177	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	6	68	theme	adhesion	1057:1064	arg1	assays					1087:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays	1018:1092	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	0	69	theme	composite	97:105	arg1	scaffold					107:114	cellulose-Ca composite scaffold	84:114	cellulose-Ca composite scaffold for carrying Schwann cells	84:141	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	6	70	dep	biocompatibility	1124:1139	arg1	the					1120:1122	the	1120:1122	the	1120:1122	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	7	71	theme	collagen/ORC-Ca	1283:1297	arg1	scaffold					1309:1316	the collagen/ORC-Ca composite scaffold	1279:1316	the collagen/ORC-Ca composite scaffold	1279:1316	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	6	72	theme	cell	1052:1055	arg1	adhesion					1057:1064	cell adhesion	1052:1064	cell adhesion	1052:1064	Cytotoxicity, cell proliferation, cell adhesion and western blotting assays are conducted to evaluate the biocompatibility and properties of different scaffolds.
30110602	5	73	theme	collagen/ORC-Ca	829:843	arg1	scaffold					855:862	the collagen/ORC-Ca composite scaffold	825:862	the collagen/ORC-Ca composite scaffold	825:862	The wettability of the collagen/ORC-Ca composite scaffold is close to that of pristine collagen, and the tensile strength of the composite scaffold (0.58 MPa) is better than that of pristine collagen (0.36 MPa).
30110602	0	74	theme	cellulose-Ca	84:95	arg1	scaffold					107:114	cellulose-Ca composite scaffold	84:114	cellulose-Ca composite scaffold for carrying Schwann cells	84:141	Fabrication, characterization and biocompatibility of collagen/oxidized regenerated cellulose-Ca composite scaffold for carrying Schwann cells.
30110602	7	75	theme	composite	1299:1307	arg1	scaffold					1309:1316	the collagen/ORC-Ca composite scaffold	1279:1316	the collagen/ORC-Ca composite scaffold	1279:1316	The results show that the three scaffolds exhibit no toxicity, and the proliferation rate of SC on the collagen/ORC-Ca composite scaffold is significantly higher than that of the other scaffolds (p < 0.05).
30110602	2	76	theme	suitable	340:347	arg1	scaffold					349:356	a suitable scaffold	338:356	a suitable scaffold for SC nerve tissue engineering	338:388	In order to develop a suitable scaffold for SC nerve tissue engineering, three kinds of scaffolds, including pristine collagen, pure oxidized regenerated cellulose-Ca (ORCCa) and collagen/ORC-Ca composite scaffolds, have been fabricated for carrying SC in this study.
30110602	8	77	theme	pure	1493:1496	arg1	p < 0.01					1512:1519	p < 0.01	1512:1519	p < 0.01	1512:1519	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30110602	8	77	theme	pure	1493:1496	arg1	group					1505:1509	the pure ORC-Ca group	1489:1509	the pure ORC-Ca group (p < 0.01)	1489:1520	The number of the adhesion cells on the composite scaffold (244.67 ± 13.02) is much more than that in the pure ORC-Ca group (p < 0.01).
30317440	7	0	theme	cultivars	1717:1725	arg1	screening					1695:1703	rapid screening	1689:1703	rapid screening of superior cultivars for heat tolerance	1689:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	4	1	theme	flowering	969:977	arg1	stage					979:983	the early flowering stage	959:983	the early flowering stage	959:983	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	2	2	from	results	516:522	arg1	future					531:536	future	531:536	future	531:536	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	5	3	theme	waxes	1302:1306	arg1	diversity					1289:1297	spectral-chemical diversity	1271:1297	spectral-chemical diversity of waxes, cutin, and polysaccharides	1271:1334	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	4	theme	cutin	1309:1313	arg1	diversity					1289:1297	spectral-chemical diversity	1271:1297	spectral-chemical diversity of waxes, cutin, and polysaccharides	1271:1334	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	6	5	theme	available	1443:1451	arg1	varieties					1463:1471	commercially available field pea varieties	1430:1471	commercially available field pea varieties	1430:1471	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	7	6	theme	rapid	1689:1693	arg1	screening					1695:1703	rapid screening	1689:1703	rapid screening of superior cultivars for heat tolerance	1689:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	3	7	theme	Infrared	653:660	arg1	spectroscopy					669:680	Infrared (FTIR) spectroscopy	653:680	Infrared (FTIR) spectroscopy	653:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	6	8	theme	leaf	1409:1412	arg1	cuticles					1414:1421	leaf cuticles	1409:1421	leaf cuticles	1409:1421	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	6	9	theme	growth	1512:1517	arg1	temperature					1519:1529	high growth temperature	1507:1529	high growth temperature	1507:1529	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	7	10	used	used	1648:1651	arg2	technique					1611:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	7	10	used	used	1648:1651	arg2	approach					1676:1683	a medium-throughput approach	1656:1683	a medium-throughput approach for rapid screening of superior cultivars for heat tolerance	1656:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	1	11	theme	spectral-chemical	191:207	arg1	composition					209:219	the spectral-chemical composition	187:219	the spectral-chemical composition of the leaf cuticle of pea	187:246	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	2	12	theme	canopy	388:393	arg1	cooling					395:401	canopy cooling	388:401	canopy cooling	388:401	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	2	13	from	role	456:459	arg1	cuticle					496:502	leaf cuticle	491:502	leaf cuticle	491:502	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	5	14	theme	main	1063:1066	arg1	region					1074:1079	the main lipid region	1059:1079	the main lipid region	1059:1079	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	14	theme	main	1063:1066	arg1	region					1086:1091	CH2 region	1082:1091	CH2 region	1082:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	3	15	theme	pea	784:786	arg1	varieties					788:796	pea varieties	784:796	pea varieties	784:796	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	6	16	theme	heat	1576:1579	arg1	stress					1581:1586	heat stress	1576:1586	heat stress	1576:1586	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	4	17	theme	heat	901:904	arg1	conditions					913:922	heat stress conditions	901:922	heat stress conditions	901:922	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	1	18	used	used	170:173	arg2	spectroscopy					102:113	ATR-FTIR spectroscopy	93:113	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis	93:164	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	5	19	theme	area	1174:1177	arg1	ratio					1179:1183	the peak area ratio	1165:1183	the peak area ratio of CH2 to CH3	1165:1197	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	20	theme	integrated	1040:1049	arg1	area					1051:1054	the integrated area	1036:1054	the integrated area of the main lipid region, CH2 region	1036:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	area					1051:1054	the integrated area	1036:1054	the integrated area of the main lipid region, CH2 region	1036:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	carbonyl					1209:1216	ester carbonyl	1203:1216	ester carbonyl	1203:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	peak					1155:1158	ester carbonyl peak	1140:1158	ester carbonyl peak	1140:1158	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	ratio					1179:1183	the peak area ratio	1165:1183	the peak area ratio of CH2 to CH3	1165:1197	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	21	from	differences	1021:1031	arg1	peak					1098:1101	CH3 peak	1094:1101	CH3 peak	1094:1101	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	6	22	theme	pea	1459:1461	arg1	varieties					1463:1471	commercially available field pea varieties	1430:1471	commercially available field pea varieties	1430:1471	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	2	23	theme	protective	445:454	arg1	role					456:459	the protective role	441:459	the protective role of lipid-related compounds in leaf cuticle	441:502	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	1	24	theme	variety	278:284	arg1	effects					267:273	the effects	263:273	the effects of variety and heat stress	263:300	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	5	25	theme	carbonyl	1146:1153	arg1	peak					1155:1158	ester carbonyl peak	1140:1158	ester carbonyl peak	1140:1158	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	0	26	theme	pea	60:62	arg1	effects					26:32	Genotypic and heat stress effects	0:32	Genotypic and heat stress effects on leaf cuticles of field pea	0:62	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	2	27	theme	leaf	491:494	arg1	cuticle					496:502	leaf cuticle	491:502	leaf cuticle	491:502	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	3	28	theme	adaxial	748:754	arg1	cuticles					756:763	adaxial cuticles	748:763	adaxial cuticles of fresh leaves of pea varieties	748:796	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	0	29	theme	ATR-FTIR	70:77	arg1	spectroscopy					79:90	ATR-FTIR spectroscopy	70:90	ATR-FTIR spectroscopy	70:90	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	5	30	theme	spectral	1012:1019	arg1	differences					1021:1031	significant spectral differences	1000:1031	significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl	1000:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	7	31	theme	ATR-FTIR	1593:1600	arg1	technique					1611:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	7	31	theme	ATR-FTIR	1593:1600	arg1	approach					1676:1683	a medium-throughput approach	1656:1683	a medium-throughput approach for rapid screening of superior cultivars for heat tolerance	1656:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	2	32	theme	compounds	478:486	arg1	role					456:459	the protective role	441:459	the protective role of lipid-related compounds in leaf cuticle	441:502	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	3	33	theme	heat	821:824	arg1	stress					826:831	heat stress	821:831	heat stress	821:831	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	0	34	from	effects	26:32	arg1	cuticles					42:49	leaf cuticles	37:49	leaf cuticles	37:49	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	2	35	theme	heat	348:351	arg1	stress					353:358	heat stress	348:358	heat stress	348:358	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	0	36	theme	Genotypic	0:8	arg1	effects					26:32	Genotypic and heat stress effects	0:32	Genotypic and heat stress effects on leaf cuticles of field pea	0:62	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	5	37	theme	CH2	1082:1084	arg1	region					1074:1079	the main lipid region	1059:1079	the main lipid region	1059:1079	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	37	theme	CH2	1082:1084	arg1	region					1086:1091	CH2 region	1082:1091	CH2 region	1082:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	1	38	from	spectroscopy	102:113	arg1	combination					118:128	combination	118:128	combination with uni- and multivariate analysis	118:164	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	4	39	theme	early	963:967	arg1	stage					979:983	the early flowering stage	959:983	the early flowering stage	959:983	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	0	40	theme	heat	14:17	arg1	stress					19:24	heat stress	14:24	heat stress	14:24	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	3	41	dep	-Fourier	634:641	arg1	Transform					643:651	Transform	643:651	Transform Infrared (FTIR) spectroscopy	643:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	1	42	theme	ATR-FTIR	93:100	arg1	spectroscopy					102:113	ATR-FTIR spectroscopy	93:113	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis	93:164	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	3	43	dep	Infrared	653:660	arg1	FTIR					663:666	FTIR	663:666	FTIR	663:666	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	1	44	theme	heat	290:293	arg1	stress					295:300	heat stress	290:300	heat stress	290:300	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	0	45	theme	leaf	37:40	arg1	cuticles					42:49	leaf cuticles	37:49	leaf cuticles	37:49	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	5	46	theme	region	1074:1079	arg1	area					1051:1054	the integrated area	1036:1054	the integrated area of the main lipid region, CH2 region	1036:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	46	theme	region	1074:1079	arg1	carbonyl					1209:1216	ester carbonyl	1203:1216	ester carbonyl	1203:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	46	theme	region	1074:1079	arg1	peak					1155:1158	ester carbonyl peak	1140:1158	ester carbonyl peak	1140:1158	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	46	theme	region	1074:1079	arg1	ratio					1179:1183	the peak area ratio	1165:1183	the peak area ratio of CH2 to CH3	1165:1197	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	46	theme	region	1074:1079	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	46	theme	region	1074:1079	arg1	peak					1098:1101	CH3 peak	1094:1101	CH3 peak	1094:1101	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	3	47	from	changes	737:743	arg1	cuticles					756:763	adaxial cuticles	748:763	adaxial cuticles of fresh leaves of pea varieties	748:796	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	5	48	theme	symmetric	1119:1127	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	3	49	theme	Total	612:616	arg1	ATR					630:632	ATR	630:632	ATR	630:632	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	3	49	theme	Total	612:616	arg1	Reflection					618:627	Total Reflection	612:627	Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy	601:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	5	50	theme	CH2	1221:1223	arg1	peak					1236:1239	CH2 asymmetric peak	1221:1239	CH2 asymmetric peak	1221:1239	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	1	51	theme	cuticle	233:239	arg1	composition					209:219	the spectral-chemical composition	187:219	the spectral-chemical composition of the leaf cuticle of pea	187:246	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	7	52	theme	superior	1708:1715	arg1	cultivars					1717:1725	superior cultivars	1708:1725	superior cultivars	1708:1725	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	5	53	theme	lipid	1068:1072	arg1	region					1074:1079	the main lipid region	1059:1079	the main lipid region	1059:1079	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	53	theme	lipid	1068:1072	arg1	region					1086:1091	CH2 region	1082:1091	CH2 region	1082:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	1	54	theme	pea	244:246	arg1	cuticle					233:239	the leaf cuticle	224:239	the leaf cuticle of pea	224:246	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	5	55	theme	asymmetric	1104:1113	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	4	56	from	stage	979:983	arg1	35/18 °C day/night					925:942	35/18 °C day/night	925:942	35/18 °C day/night	925:942	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	7	57	theme	medium-throughput	1658:1674	arg1	technique					1611:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	7	57	theme	medium-throughput	1658:1674	arg1	approach					1676:1683	a medium-throughput approach	1656:1683	a medium-throughput approach for rapid screening of superior cultivars for heat tolerance	1656:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	5	58	theme	spectral-chemical	1271:1287	arg1	diversity					1289:1297	spectral-chemical diversity	1271:1297	spectral-chemical diversity of waxes, cutin, and polysaccharides	1271:1334	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	2	59	theme	Field	303:307	arg1	Pisum					314:318	Pisum	314:318	Pisum	314:318	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	2	59	theme	Field	303:307	arg1	pea					309:311	Field pea	303:311	Field pea (Pisum sativum L.)	303:330	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	6	60	theme	cuticles	1414:1421	arg1	makeup					1399:1404	spectral-chemical makeup	1381:1404	spectral-chemical makeup of leaf cuticles within commercially available field pea varieties	1381:1471	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	2	61	theme	resistant	555:563	arg1	genotypes					565:573	heat resistant genotypes	550:573	heat resistant genotypes	550:573	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	3	62	theme	non-invasive	685:696	arg1	-Fourier					634:641	Attenuated Total Reflection (ATR)-Fourier	601:641	Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy	601:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	3	62	theme	non-invasive	685:696	arg1	technique					698:706	a non-invasive technique	683:706	a non-invasive technique	683:706	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	6	63	theme	spectral-chemical	1381:1397	arg1	makeup					1399:1404	spectral-chemical makeup	1381:1404	spectral-chemical makeup of leaf cuticles within commercially available field pea varieties	1381:1471	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	5	64	theme	significant	1000:1010	arg1	differences					1021:1031	significant spectral differences	1000:1031	significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl	1000:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	3	65	theme	varieties	788:796	arg1	fresh					768:772	fresh	768:772	fresh	768:772	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	5	66	theme	peak	1169:1172	arg1	ratio					1179:1183	the peak area ratio	1165:1183	the peak area ratio of CH2 to CH3	1165:1197	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	0	67	theme	field	54:58	arg1	pea					60:62	field pea	54:62	field pea	54:62	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	3	68	dep	fresh	768:772	arg1	leaves					774:779	leaves	774:779	leaves	774:779	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	5	69	theme	ester	1140:1144	arg1	peak					1155:1158	ester carbonyl peak	1140:1158	ester carbonyl peak	1140:1158	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	4	70	dep	grown	856:860	arg1	35/18 °C day/night					925:942	35/18 °C day/night	925:942	35/18 °C day/night	925:942	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	6	71	theme	field	1453:1457	arg1	varieties					1463:1471	commercially available field pea varieties	1430:1471	commercially available field pea varieties	1430:1471	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	1	72	theme	multivariate	144:155	arg1	analysis					157:164	multivariate analysis	144:164	multivariate analysis	144:164	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	4	73	theme	stress	906:911	arg1	conditions					913:922	heat stress conditions	901:922	heat stress conditions	901:922	Eleven varieties were grown under control (24/18 °C day/night) and heat stress conditions (35/18 °C day/night, for 5 days at the early flowering stage).
30317440	3	74	theme	fresh	768:772	arg1	cuticles					756:763	adaxial cuticles	748:763	adaxial cuticles of fresh leaves of pea varieties	748:796	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	6	75	theme	diverse	1548:1554	arg1	potential					1556:1564	their diverse potential to resist heat stress	1542:1586	their diverse potential to resist heat stress	1542:1586	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	1	76	with	combination	118:128	arg1	uni-					135:138	uni-	135:138	uni-	135:138	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	1	76	with	combination	118:128	arg1	analysis					157:164	multivariate analysis	144:164	multivariate analysis	144:164	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	7	77	theme	spectral	1602:1609	arg1	technique					1611:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique	1589:1619	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	7	77	theme	spectral	1602:1609	arg1	approach					1676:1683	a medium-throughput approach	1656:1683	a medium-throughput approach for rapid screening of superior cultivars for heat tolerance	1656:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	2	78	theme	lipid-related	464:476	arg1	compounds					478:486	lipid-related compounds	464:486	lipid-related compounds	464:486	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	5	79	theme	polysaccharides	1320:1334	arg1	diversity					1289:1297	spectral-chemical diversity	1271:1297	spectral-chemical diversity of waxes, cutin, and polysaccharides	1271:1334	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	1	80	theme	leaf	228:231	arg1	cuticle					233:239	the leaf cuticle	224:239	the leaf cuticle of pea	224:246	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	5	81	theme	ester	1203:1207	arg1	carbonyl					1209:1216	ester carbonyl	1203:1216	ester carbonyl	1203:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	82	contain	had	996:998	arg1	11					993:994	11	993:994	11	993:994	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	82	contain	had	996:998	arg2	differences					1021:1031	significant spectral differences	1000:1031	significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl	1000:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	0	83	theme	stress	19:24	arg1	effects					26:32	Genotypic and heat stress effects	0:32	Genotypic and heat stress effects on leaf cuticles of field pea	0:62	Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.
30317440	6	84	theme	high	1507:1510	arg1	temperature					1519:1529	high growth temperature	1507:1529	high growth temperature	1507:1529	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	2	85	dep	Pisum	314:318	arg1	L.					328:329	Pisum sativum L.	314:329	Pisum sativum L.	314:329	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	5	86	theme	CH2	1188:1190	arg1	area					1051:1054	the integrated area	1036:1054	the integrated area of the main lipid region, CH2 region	1036:1091	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	86	theme	CH2	1188:1190	arg1	carbonyl					1209:1216	ester carbonyl	1203:1216	ester carbonyl	1203:1216	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	86	theme	CH2	1188:1190	arg1	peak					1155:1158	ester carbonyl peak	1140:1158	ester carbonyl peak	1140:1158	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	86	theme	CH2	1188:1190	arg1	ratio					1179:1183	the peak area ratio	1165:1183	the peak area ratio of CH2 to CH3	1165:1197	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	86	theme	CH2	1188:1190	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	86	theme	CH2	1188:1190	arg1	peak					1098:1101	CH3 peak	1094:1101	CH3 peak	1094:1101	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	87	theme	CH3	1094:1096	arg1	peak					1098:1101	CH3 peak	1094:1101	CH3 peak	1094:1101	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	88	contain	had	1267:1269	arg2	diversity					1289:1297	spectral-chemical diversity	1271:1297	spectral-chemical diversity of waxes, cutin, and polysaccharides	1271:1334	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	5	88	contain	had	1267:1269	arg1	cuticles					1258:1265	cuticles	1258:1265	cuticles	1258:1265	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	1	89	theme	stress	295:300	arg1	effects					267:273	the effects	263:273	the effects of variety and heat stress	263:300	ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress.
30317440	2	90	theme	flower	407:412	arg1	retention					414:422	flower retention	407:422	flower retention	407:422	Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy cooling and flower retention by investigating the protective role of lipid-related compounds in leaf cuticle, and to use results in the future to identify heat resistant genotypes.
30317440	3	91	theme	Reflection	618:627	arg1	-Fourier					634:641	Attenuated Total Reflection (ATR)-Fourier	601:641	Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy	601:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	3	91	theme	Reflection	618:627	arg1	technique					698:706	a non-invasive technique	683:706	a non-invasive technique	683:706	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	3	92	theme	Attenuated	601:610	arg1	-Fourier					634:641	Attenuated Total Reflection (ATR)-Fourier	601:641	Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy	601:680	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	3	92	theme	Attenuated	601:610	arg1	technique					698:706	a non-invasive technique	683:706	a non-invasive technique	683:706	The objective was to use Attenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, a non-invasive technique, to investigate and quantify changes in adaxial cuticles of fresh leaves of pea varieties that were subjected to heat stress.
30317440	6	93	theme	considerable	1355:1366	arg1	diversity					1368:1376	considerable diversity	1355:1376	considerable diversity	1355:1376	Results indicated considerable diversity in spectral-chemical makeup of leaf cuticles within commercially available field pea varieties and they responded differently to high growth temperature, revealing their diverse potential to resist heat stress.
30317440	5	94	theme	CH2	1129:1131	arg1	peaks					1133:1137	asymmetric and symmetric CH2 peaks	1104:1137	asymmetric and symmetric CH2 peaks	1104:1137	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30317440	7	95	theme	heat	1731:1734	arg1	tolerance					1736:1744	heat tolerance	1731:1744	heat tolerance	1731:1744	The ATR-FTIR spectral technique can, therefore, be further used as a medium-throughput approach for rapid screening of superior cultivars for heat tolerance.
30317440	5	96	theme	asymmetric	1225:1234	arg1	peak					1236:1239	CH2 asymmetric peak	1221:1239	CH2 asymmetric peak	1221:1239	These 11 had significant spectral differences in the integrated area of the main lipid region, CH2 region, CH3 peak, asymmetric and symmetric CH2 peaks, ester carbonyl peak, and the peak area ratio of CH2 to CH3 and ester carbonyl to CH2 asymmetric peak, indicating that cuticles had spectral-chemical diversity of waxes, cutin, and polysaccharides.
30502431	1	0	theme	structures	341:350	arg1	aspects					296:302	all aspects	292:302	all aspects	292:302	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	1	1	theme	sites	174:178	arg1	engineering					150:160	tissue engineering	143:160	tissue engineering of specific sites such as cardiac, nerve, and bone tissues	143:219	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	2	2	theme	engineering	501:511	arg1	applications					513:524	tissue engineering applications	494:524	tissue engineering applications	494:524	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	3	3	from	polysaccharides	584:598	arg1	Improvement					527:537	Improvement	527:537	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	527:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	3	from	polysaccharides	584:598	arg1	goal					677:680	the main goal	668:680	the main goal of this study	668:694	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	3	from	polysaccharides	584:598	arg1	hydrogel					556:563	an injectable hydrogel	542:563	an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	542:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	0	4	theme	engineering	108:118	arg1	demands					120:126	tissue engineering demands	101:126	tissue engineering demands	101:126	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	5	5	theme	hydrogel	950:957	arg1	properties					921:930	Chemical and physical properties	899:930	Chemical and physical properties of the injectable hydrogel	899:957	Chemical and physical properties of the injectable hydrogel are fully characterized.
30502431	6	6	theme	viability	1112:1120	arg1	assay					1122:1126	MG-63 cell viability assay	1101:1126	MG-63 cell viability assay	1101:1126	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	1	7	theme	dimensional	258:268	arg1	materials					270:278	three dimensional materials	252:278	three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells	252:394	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	0	8	theme	tissue	101:106	arg1	demands					120:126	tissue engineering demands	101:126	tissue engineering demands	101:126	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	4	9	theme	nanoparticles	798:810	arg1	addition					783:790	addition	783:790	addition of Au nanoparticles	783:810	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	4	9	theme	nanoparticles	798:810	arg1	design					754:759	mixture design	746:759	mixture design of the components	746:777	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	6	10	theme	nanoparticles	999:1011	arg1	Addition					984:991	Addition	984:991	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment	984:1075	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	4	11	theme	mixture	746:752	arg1	design					754:759	mixture design	746:759	mixture design of the components	746:777	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	1	12	theme	materials	270:278	arg1	design					242:247	a comprehensive design	226:247	a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells	226:394	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	5	13	theme	physical	912:919	arg1	properties					921:930	Chemical and physical properties	899:930	Chemical and physical properties of the injectable hydrogel	899:957	Chemical and physical properties of the injectable hydrogel are fully characterized.
30502431	0	14	theme	au	57:58	arg1	nanoparticles					60:72	au nanoparticles	57:72	au nanoparticles	57:72	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	3	15	theme	biocompatible	570:582	arg1	polysaccharides					584:598	biocompatible polysaccharides	570:598	biocompatible polysaccharides	570:598	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	4	16	from	enhancements	706:717	arg1	study					727:731	this study	722:731	this study	722:731	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	4	17	theme	main	701:704	arg1	enhancements					706:717	Two main enhancements	697:717	Two main enhancements in this study	697:731	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	0	18	theme	chitosan/κ-carrageenan	11:32	arg1	hydrogel					34:41	Injectable chitosan/κ-carrageenan hydrogel	0:41	Injectable chitosan/κ-carrageenan hydrogel	0:41	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	1	19	theme	desired	382:388	arg1	cells					390:394	desired cells	382:394	desired cells	382:394	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	0	20	theme	Injectable	0:9	arg1	hydrogel					34:41	Injectable chitosan/κ-carrageenan hydrogel	0:41	Injectable chitosan/κ-carrageenan hydrogel	0:41	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	3	21	from	Improvement	527:537	arg1	polysaccharides					584:598	biocompatible polysaccharides	570:598	biocompatible polysaccharides	570:598	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	21	from	Improvement	527:537	arg1	amide					627:631	poly‑N‑isopropyl acryl amide	604:631	poly‑N‑isopropyl acryl amide	604:631	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	22	theme	poly‑N‑isopropyl	604:619	arg1	amide					627:631	poly‑N‑isopropyl acryl amide	604:631	poly‑N‑isopropyl acryl amide	604:631	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	2	23	theme	interconnected	439:452	arg1	structures					454:463	highly hydrated and interconnected structures	419:463	highly hydrated and interconnected structures	419:463	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	1	24	theme	cells	390:394	arg1	regeneration					366:377	regeneration	366:377	regeneration of desired cells	366:394	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	3	25	theme	acryl	621:625	arg1	amide					627:631	poly‑N‑isopropyl acryl amide	604:631	poly‑N‑isopropyl acryl amide	604:631	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	5	26	theme	injectable	939:948	arg1	hydrogel					950:957	the injectable hydrogel	935:957	the injectable hydrogel	935:957	Chemical and physical properties of the injectable hydrogel are fully characterized.
30502431	2	27	theme	hydrated	426:433	arg1	structures					454:463	highly hydrated and interconnected structures	419:463	highly hydrated and interconnected structures	419:463	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	2	28	theme	tissue	494:499	arg1	applications					513:524	tissue engineering applications	494:524	tissue engineering applications	494:524	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	6	29	theme	conductive	1018:1027	arg1	component					1029:1037	a conductive component	1016:1037	a conductive component to enhance cell growth and attachment	1016:1075	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	5	30	theme	Chemical	899:906	arg1	properties					921:930	Chemical and physical properties	899:930	Chemical and physical properties of the injectable hydrogel	899:957	Chemical and physical properties of the injectable hydrogel are fully characterized.
30502431	1	31	theme	bone	208:211	arg1	tissues					213:219	bone tissues	208:219	bone tissues	208:219	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	6	32	dep	cell	1050:1053	arg1	growth					1055:1060	growth	1055:1060	growth	1055:1060	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	4	33	theme	potential	854:862	arg1	application					864:874	potential application	854:874	potential application	854:874	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	6	34	theme	cell	1107:1110	arg1	assay					1122:1126	MG-63 cell viability assay	1101:1126	MG-63 cell viability assay	1101:1126	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	1	35	theme	chemical	307:314	arg1	composition					316:326	chemical composition	307:326	chemical composition	307:326	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	3	36	theme	study	690:694	arg1	Improvement					527:537	Improvement	527:537	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	527:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	36	theme	study	690:694	arg1	goal					677:680	the main goal	668:680	the main goal of this study	668:694	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	4	37	theme	tissue	879:884	arg1	engineering					886:896	tissue engineering	879:896	tissue engineering	879:896	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	2	38	theme	promising	470:478	arg1	Hydrogels					397:405	Hydrogels	397:405	Hydrogels	397:405	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	2	38	theme	promising	470:478	arg1	materials					480:488	promising materials	470:488	promising materials for tissue engineering applications	470:524	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	1	39	theme	composition	316:326	arg1	aspects					296:302	all aspects	292:302	all aspects	292:302	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	2	40	contain	possessing	408:417	arg1	Hydrogels					397:405	Hydrogels	397:405	Hydrogels	397:405	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	2	40	contain	possessing	408:417	arg1	materials					480:488	promising materials	470:488	promising materials for tissue engineering applications	470:524	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	2	40	contain	possessing	408:417	arg2	structures					454:463	highly hydrated and interconnected structures	419:463	highly hydrated and interconnected structures	419:463	Hydrogels, possessing highly hydrated and interconnected structures, are promising materials for tissue engineering applications.
30502431	3	41	theme	Au	647:648	arg1	nanoparticles					650:662	Au nanoparticles	647:662	Au nanoparticles	647:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	4	42	theme	components	768:777	arg1	addition					783:790	addition	783:790	addition of Au nanoparticles	783:810	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	4	42	theme	components	768:777	arg1	design					754:759	mixture design	746:759	mixture design of the components	746:777	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	6	43	theme	Au	996:997	arg1	nanoparticles					999:1011	Au nanoparticles	996:1011	Au nanoparticles as a conductive component to enhance cell growth and attachment	996:1075	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	1	44	theme	tissue	143:148	arg1	engineering					150:160	tissue engineering	143:160	tissue engineering of specific sites such as cardiac, nerve, and bone tissues	143:219	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	4	45	theme	Au	795:796	arg1	nanoparticles					798:810	Au nanoparticles	795:810	Au nanoparticles	795:810	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	6	46	theme	MG-63	1101:1105	arg1	assay					1122:1126	MG-63 cell viability assay	1101:1126	MG-63 cell viability assay	1101:1126	Addition of Au nanoparticles as a conductive component to enhance cell growth and attachment is investigated through MG-63 cell viability assay.
30502431	4	47	theme	homogeneous	824:834	arg1	mixture					836:842	a homogeneous mixture	822:842	a homogeneous mixture that have potential application in tissue engineering	822:896	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	3	48	theme	hydrogel	556:563	arg1	Improvement					527:537	Improvement	527:537	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	527:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	48	theme	hydrogel	556:563	arg1	goal					677:680	the main goal	668:680	the main goal of this study	668:694	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	49	from	amide	627:631	arg1	Improvement					527:537	Improvement	527:537	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	527:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	49	from	amide	627:631	arg1	goal					677:680	the main goal	668:680	the main goal of this study	668:694	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	49	from	amide	627:631	arg1	hydrogel					556:563	an injectable hydrogel	542:563	an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	542:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	4	50	contain	have	849:852	arg2	application					864:874	potential application	854:874	potential application	854:874	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	4	50	contain	have	849:852	arg1	mixture					836:842	a homogeneous mixture	822:842	a homogeneous mixture that have potential application in tissue engineering	822:896	Two main enhancements in this study are included mixture design of the components and addition of Au nanoparticles to access a homogeneous mixture that have potential application in tissue engineering.
30502431	0	51	theme	conductive	77:86	arg1	scaffold					88:95	A conductive scaffold	75:95	A conductive scaffold for tissue engineering demands	75:126	Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands.
30502431	3	52	theme	injectable	545:554	arg1	hydrogel					556:563	an injectable hydrogel	542:563	an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	542:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	1	53	theme	comprehensive	228:240	arg1	design					242:247	a comprehensive design	226:247	a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells	226:394	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	1	54	theme	physical	332:339	arg1	structures					341:350	physical structures	332:350	physical structures	332:350	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	3	55	theme	main	672:675	arg1	Improvement					527:537	Improvement	527:537	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles	527:662	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	3	55	theme	main	672:675	arg1	goal					677:680	the main goal	668:680	the main goal of this study	668:694	Improvement of an injectable hydrogel from biocompatible polysaccharides and poly‑N‑isopropyl acryl amide enriched with Au nanoparticles are the main goal of this study.
30502431	1	56	theme	specific	165:172	arg1	nerve					197:201	nerve	197:201	nerve	197:201	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	1	56	theme	specific	165:172	arg1	sites					174:178	specific sites	165:178	specific sites such as cardiac, nerve, and bone tissues	165:219	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	1	56	theme	specific	165:172	arg1	cardiac					188:194	cardiac	188:194	cardiac	188:194	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
30502431	1	56	theme	specific	165:172	arg1	tissues					213:219	bone tissues	208:219	bone tissues	208:219	Scaffolds for tissue engineering of specific sites such as cardiac, nerve, and bone tissues need a comprehensive design of three dimensional materials that covers all aspects of chemical composition and physical structures, required for regeneration of desired cells.
31201836	10	0	theme	outlet	1439:1444	arg1	temperature					1446:1456	high outlet temperature	1434:1456	high outlet temperature (≈70 °C)	1434:1465	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	10	0	theme	outlet	1439:1444	arg1	≈70 °C					1459:1464	≈70 °C	1459:1464	≈70 °C	1459:1464	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	5	1	from	40 °C	730:734	arg1	stable					720:725	stable	720:725	stable	720:725	Both strains were stable at 40 °C and completely died after five minutes at 80 °C, while CC275e was more stable than TA1 at 60 °C. TA1 and CC275e slightly decreased viability after six hours drying with either carriers, with no differences between strains.
31201836	10	2	theme	negative	1398:1405	arg1	effects					1407:1413	the negative effects	1394:1413	the negative effects of dehydration and high outlet temperature (≈70 °C)	1394:1465	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	10	3	theme	high	1434:1437	arg1	temperature					1446:1456	high outlet temperature	1434:1456	high outlet temperature (≈70 °C)	1434:1465	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	10	3	theme	high	1434:1437	arg1	≈70 °C					1459:1464	≈70 °C	1459:1464	≈70 °C	1459:1464	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	2	4	theme	Rhizobium	115:123	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	4	theme	Rhizobium	115:123	arg1	CC275e					167:172	CC275e	167:172	CC275e	167:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	4	theme	Rhizobium	115:123	arg1	TA1					159:161	TA1	159:161	TA1	159:161	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	4	theme	Rhizobium	115:123	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	5	theme	stress	330:335	arg1	conditions					337:346	different stress conditions	320:346	different stress conditions	320:346	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	7	6	theme	feed	1097:1100	arg1	5 mL/min					1113:1120	5 mL/min	1113:1120	5 mL/min	1113:1120	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	7	6	theme	feed	1097:1100	arg1	rate					1107:1110	feed flow rate	1097:1110	feed flow rate (5 mL/min)	1097:1121	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	4	7	dep	strains	590:596	arg1	strains					590:596	strains TA1 and CC275e	590:611	strains TA1 and CC275e grown on peat and diatomaceous earth	590:648	First, strains TA1 and CC275e grown on peat and diatomaceous earth were exposed to temperature and desiccation stress.
31201836	4	7	dep	strains	590:596	arg1	CC275e					606:611	CC275e	606:611	CC275e	606:611	First, strains TA1 and CC275e grown on peat and diatomaceous earth were exposed to temperature and desiccation stress.
31201836	4	7	dep	strains	590:596	arg1	TA1					598:600	TA1	598:600	TA1	598:600	First, strains TA1 and CC275e grown on peat and diatomaceous earth were exposed to temperature and desiccation stress.
31201836	1	8	theme	guar	64:67	arg1	gum					69:71	guar gum	64:71	guar gum	64:71	trifolii with guar gum: Preliminary approach using spray drying.
31201836	8	9	from	loading	1158:1164	arg1	size					1197:1200	mean particle size	1183:1200	mean particle size	1183:1200	Microparticles presented rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm.
31201836	8	9	from	loading	1158:1164	arg1	CFU/g					1173:1177	107 CFU/g	1169:1177	107 CFU/g	1169:1177	Microparticles presented rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm.
31201836	7	10	theme	flow	1102:1105	arg1	5 mL/min					1113:1120	5 mL/min	1113:1120	5 mL/min	1113:1120	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	7	10	theme	flow	1102:1105	arg1	rate					1107:1110	feed flow rate	1097:1110	feed flow rate (5 mL/min)	1097:1121	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	10	11	theme	logarithmic	1314:1324	arg1	units					1326:1330	two logarithmic units	1310:1330	two logarithmic units	1310:1330	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	2	12	theme	rhizobia	270:277	arg1	survival					279:286	rhizobia survival	270:286	rhizobia survival on seeds	270:295	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	5	13	theme	60 °C.	826:831	arg1	TA1					833:835	60 °C. TA1	826:835	60 °C. TA1	826:835	Both strains were stable at 40 °C and completely died after five minutes at 80 °C, while CC275e was more stable than TA1 at 60 °C. TA1 and CC275e slightly decreased viability after six hours drying with either carriers, with no differences between strains.
31201836	8	14	theme	mean	1183:1186	arg1	size					1197:1200	mean particle size	1183:1200	mean particle size	1183:1200	Microparticles presented rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm.
31201836	10	15	theme	drying	1563:1568	arg1	chamber					1570:1576	the drying chamber	1559:1576	the drying chamber	1559:1576	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	5	16	from	TA1	833:835	arg1	stable					807:812	stable	807:812	stable	807:812	Both strains were stable at 40 °C and completely died after five minutes at 80 °C, while CC275e was more stable than TA1 at 60 °C. TA1 and CC275e slightly decreased viability after six hours drying with either carriers, with no differences between strains.
31201836	3	17	theme	commercial	398:407	arg1	strains					409:415	those commercial strains	392:415	those commercial strains grown in a solid carrier (core)	392:447	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	1	18	theme	Preliminary	74:84	arg1	approach					86:93	Preliminary approach	74:93	trifolii with guar gum: Preliminary approach using spray drying.	50:113	trifolii with guar gum: Preliminary approach using spray drying.
31201836	3	19	theme	strains	409:415	arg1	strains					409:415	those commercial strains	392:415	those commercial strains grown in a solid carrier (core)	392:447	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	3	19	theme	strains	409:415	arg1	one					385:387	one	385:387	one	385:387	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	2	20	theme	trifolii	142:149	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	20	theme	trifolii	142:149	arg1	CC275e					167:172	CC275e	167:172	CC275e	167:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	20	theme	trifolii	142:149	arg1	TA1					159:161	TA1	159:161	TA1	159:161	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	20	theme	trifolii	142:149	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	21	used	used	191:194	arg2	CC275e					167:172	CC275e	167:172	CC275e	167:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	21	used	used	191:194	arg2	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	21	used	used	191:194	arg2	TA1					159:161	TA1	159:161	TA1	159:161	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	21	used	used	191:194	arg2	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	6	22	theme	central	961:967	arg1	design					979:984	A central composite design	959:984	A central composite design	959:984	A central composite design was used to develop the microencapsulation process.
31201836	3	23	theme	study	365:369	arg1	aim					353:355	The aim	349:355	The aim of this study	349:369	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	2	24	dep	strains	151:157	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	24	dep	strains	151:157	arg1	CC275e					167:172	CC275e	167:172	CC275e	167:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	24	dep	strains	151:157	arg1	TA1					159:161	TA1	159:161	TA1	159:161	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	10	25	theme	microencapsulation/drying	1343:1367	arg1	process					1369:1375	the microencapsulation/drying process	1339:1375	the microencapsulation/drying process	1339:1375	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	4	26	theme	desiccation	682:692	arg1	stress					694:699	desiccation stress	682:699	desiccation stress	682:699	First, strains TA1 and CC275e grown on peat and diatomaceous earth were exposed to temperature and desiccation stress.
31201836	0	27	theme	leguminosarum	32:44	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of Rhizobium leguminosarum bv.	0:48	Microencapsulation of Rhizobium leguminosarum bv.
31201836	9	28	theme	Optimized	1224:1232	arg1	process					1234:1240	Optimized process	1224:1240	Optimized process	1224:1240	Optimized process reached 50% yield and 107 CFU/g loading.
31201836	8	29	theme	particle	1188:1195	arg1	size					1197:1200	mean particle size	1183:1200	mean particle size	1183:1200	Microparticles presented rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm.
31201836	10	30	theme	Rhizobia	1283:1290	arg1	viability					1292:1300	Rhizobia viability	1283:1300	Rhizobia viability	1283:1300	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	1	31	theme	spray	101:105	arg1	drying					107:112	spray drying	101:112	spray drying	101:112	trifolii with guar gum: Preliminary approach using spray drying.
31201836	2	32	from	clover	243:248	arg1	Zealand					257:263	New Zealand	253:263	New Zealand	253:263	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	1	33	dep	trifolii	50:57	arg1	approach					86:93	Preliminary approach	74:93	trifolii with guar gum: Preliminary approach using spray drying.	50:113	trifolii with guar gum: Preliminary approach using spray drying.
31201836	0	34	theme	Rhizobium	22:30	arg1	leguminosarum					32:44	Rhizobium leguminosarum bv	22:47	Rhizobium leguminosarum bv	22:47	Microencapsulation of Rhizobium leguminosarum bv.
31201836	10	35	theme	thermal	1540:1546	arg1	profile					1548:1554	the thermal profile	1536:1554	the thermal profile in the drying chamber	1536:1576	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	3	36	theme	ratio	493:497	arg1	influence					463:471	the influence	459:471	the influence of the core:polymer ratio	459:497	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	3	37	theme	solid	428:432	arg1	core					443:446	core	443:446	core	443:446	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	3	37	theme	solid	428:432	arg1	carrier					434:440	a solid carrier	426:440	a solid carrier (core)	426:447	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	2	38	theme	white	237:241	arg1	clover					243:248	white clover	237:248	white clover in New Zealand	237:263	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	39	dep	Rhizobium	115:123	arg1	leguminosarum					125:137	leguminosarum	125:137	leguminosarum	125:137	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	3	40	theme	spray	534:538	arg1	drying					540:545	spray drying	534:545	spray drying using guar gum as coating material	534:580	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	5	41	dep	drying	893:898	arg1	carriers					912:919	either carriers	905:919	either carriers	905:919	Both strains were stable at 40 °C and completely died after five minutes at 80 °C, while CC275e was more stable than TA1 at 60 °C. TA1 and CC275e slightly decreased viability after six hours drying with either carriers, with no differences between strains.
31201836	7	42	theme	Independent	1038:1048	arg1	variables					1050:1058	Independent variables	1038:1058	Independent variables	1038:1058	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	9	43	theme	50	1250:1251	arg1	%					1252:1252	%	1252:1252	%	1252:1252	Optimized process reached 50% yield and 107 CFU/g loading.
31201836	3	44	theme	coating	565:571	arg1	material					573:580	coating material	565:580	coating material	565:580	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	3	45	theme	microencapsulation	504:521	arg1	process					523:529	a microencapsulation process	502:529	a microencapsulation process	502:529	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	6	46	used	used	990:993	arg2	design					979:984	A central composite design	959:984	A central composite design	959:984	A central composite design was used to develop the microencapsulation process.
31201836	2	47	theme	different	320:328	arg1	conditions					337:346	different stress conditions	320:346	different stress conditions	320:346	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	10	48	theme	dehydration	1418:1428	arg1	effects					1407:1413	the negative effects	1394:1413	the negative effects of dehydration and high outlet temperature (≈70 °C)	1394:1465	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	2	49	theme	nitrogen	209:216	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	49	theme	nitrogen	209:216	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	50	theme	fixing	218:223	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	50	theme	fixing	218:223	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	10	51	from	profile	1548:1554	arg1	chamber					1570:1576	the drying chamber	1559:1576	the drying chamber	1559:1576	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	7	52	theme	inlet	1066:1070	arg1	temperature					1072:1082	inlet temperature	1066:1082	inlet temperature (130 °C)	1066:1091	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	7	52	theme	inlet	1066:1070	arg1	130 °C					1085:1090	130 °C	1085:1090	130 °C	1085:1090	Independent variables were: inlet temperature (130 °C) and feed flow rate (5 mL/min).
31201836	2	53	theme	effective	199:207	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	53	theme	effective	199:207	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	3	54	theme	guar	553:556	arg1	gum					558:560	guar gum	553:560	guar gum	553:560	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	8	55	theme	rhizobia	1149:1156	arg1	loading					1158:1164	rhizobia loading	1149:1164	rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm	1149:1221	Microparticles presented rhizobia loading in 107 CFU/g and mean particle size between 10 and 30 μm.
31201836	6	56	theme	microencapsulation	1010:1027	arg1	process					1029:1035	the microencapsulation process	1006:1035	the microencapsulation process	1006:1035	A central composite design was used to develop the microencapsulation process.
31201836	3	57	theme	polymer	485:491	arg1	ratio					493:497	the core:polymer ratio	476:497	the core:polymer ratio	476:497	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	9	58	theme	107	1264:1266	arg1	CFU/g					1268:1272	CFU/g	1268:1272	CFU/g	1268:1272	Optimized process reached 50% yield and 107 CFU/g loading.
31201836	2	59	theme	New	253:255	arg1	Zealand					257:263	New Zealand	253:263	New Zealand	253:263	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	4	60	theme	diatomaceous	631:642	arg1	earth					644:648	diatomaceous earth	631:648	diatomaceous earth	631:648	First, strains TA1 and CC275e grown on peat and diatomaceous earth were exposed to temperature and desiccation stress.
31201836	2	61	from	survival	279:286	arg1	seeds					291:295	seeds	291:295	seeds	291:295	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	9	62	theme	CFU/g	1268:1272	arg1	loading					1274:1280	107 CFU/g loading	1264:1280	107 CFU/g loading	1264:1280	Optimized process reached 50% yield and 107 CFU/g loading.
31201836	1	63	with	trifolii	50:57	arg1	gum					69:71	guar gum	64:71	guar gum	64:71	trifolii with guar gum: Preliminary approach using spray drying.
31201836	3	64	theme	core	480:483	arg1	ratio					493:497	the core:polymer ratio	476:497	the core:polymer ratio	476:497	The aim of this study was to select one of those commercial strains grown in a solid carrier (core) and study the influence of the core:polymer ratio in a microencapsulation process by spray drying using guar gum as coating material.
31201836	6	65	theme	composite	969:977	arg1	design					979:984	A central composite design	959:984	A central composite design	959:984	A central composite design was used to develop the microencapsulation process.
31201836	10	66	theme	temperature	1446:1456	arg1	effects					1407:1413	the negative effects	1394:1413	the negative effects of dehydration and high outlet temperature (≈70 °C)	1394:1465	Rhizobia viability dropped two logarithmic units during the microencapsulation/drying process, possibly due to the negative effects of dehydration and high outlet temperature (≈70 °C), suggesting the need to continue optimizing the process by improving the thermal profile in the drying chamber.
31201836	2	67	theme	bv	139:140	arg1	strains					151:157	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e	115:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	67	theme	bv	139:140	arg1	CC275e					167:172	CC275e	167:172	CC275e	167:172	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	67	theme	bv	139:140	arg1	TA1					159:161	TA1	159:161	TA1	159:161	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	2	67	theme	bv	139:140	arg1	strains					225:231	effective nitrogen fixing strains	199:231	effective nitrogen fixing strains	199:231	Rhizobium leguminosarum bv trifolii strains TA1 and CC275e have been widely used as effective nitrogen fixing strains for white clover in New Zealand, but rhizobia survival on seeds is usually poor due to different stress conditions.
31201836	9	68	theme	%	1252:1252	arg1	yield					1254:1258	50% yield	1250:1258	50% yield	1250:1258	Optimized process reached 50% yield and 107 CFU/g loading.
30959773	5	0	theme	anti-tumor	790:799	arg1	effects					801:807	anti-tumor effects	790:807	anti-tumor effects	790:807	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	0	1	from	Structure	0:8	arg1	Alternaria					63:72	Alternaria	63:72	Alternaria	63:72	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	1	2	from	AMEP	165:168	arg1	polysaccharide					120:133	an extracellular polysaccharide	103:133	an extracellular polysaccharide from Alternaria mali Roberts (AMEP)	103:169	In this study, an extracellular polysaccharide from Alternaria mali Roberts (AMEP) was extracted, and its structure was characterized, in addition to its antitumor activity in vitro.
30959773	3	3	theme	linking	490:496	arg1	Manp-					532:536	Manp-	532:536	Manp-(1→4)	532:541	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	3	3	theme	linking	490:496	arg1	mode					498:501	The linking mode	486:501	The linking mode of both AMEP-1 and AMEP-2	486:527	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	5	4	theme	production	879:888	arg1	reduction					928:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction	842:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells	842:953	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	1	5	theme	antitumor	242:250	arg1	activity					252:259	its antitumor activity	238:259	its antitumor activity in vitro	238:268	In this study, an extracellular polysaccharide from Alternaria mali Roberts (AMEP) was extracted, and its structure was characterized, in addition to its antitumor activity in vitro.
30959773	2	6	theme	anionic	305:311	arg1	AMEP-2					328:333	anionic polysaccharide AMEP-2	305:333	anionic polysaccharide AMEP-2	305:333	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	0	7	from	Alternaria	63:72	arg1	Exopolysaccharides					39:56	Exopolysaccharides	39:56	Exopolysaccharides from Alternaria	39:72	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	0	7	from	Alternaria	63:72	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	0	7	from	Alternaria	63:72	arg1	Activities					25:34	Anti-Tumor Activities	14:34	Anti-Tumor Activities	14:34	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	5	8	theme	cycle	826:830	arg1	arrest					832:837	cell cycle arrest	821:837	cell cycle arrest	821:837	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	2	9	attach	isolated	340:347	arg1	AMEP					354:357	AMEP	354:357	AMEP	354:357	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	2	9	attach	isolated	340:347	arg2	AMEP-1					294:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	2	9	attach	isolated	340:347	arg2	AMEP-2					328:333	anionic polysaccharide AMEP-2	305:333	anionic polysaccharide AMEP-2	305:333	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	4	10	theme	cells	674:678	arg1	proliferation					649:661	the proliferation	645:661	the proliferation of BGC-823 cells	645:678	AMEP-2 inhibited the proliferation of BGC-823 cells in a time- and concentration-dependent manner.
30959773	5	11	theme	cells	772:776	arg1	apoptosis					751:759	the apoptosis	747:759	the apoptosis of BGC-823 cells	747:776	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	3	12	theme	main	604:607	arg1	chain					609:613	the main chain	600:613	the main chain	600:613	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	4	13	theme	BGC-823	666:672	arg1	cells					674:678	BGC-823 cells	666:678	BGC-823 cells	666:678	AMEP-2 inhibited the proliferation of BGC-823 cells in a time- and concentration-dependent manner.
30959773	5	14	theme	BGC-823	764:770	arg1	cells					772:776	BGC-823 cells	764:776	BGC-823 cells	764:776	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	3	15	theme	AMEP-1	511:516	arg1	Manp-					532:536	Manp-	532:536	Manp-(1→4)	532:541	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	3	15	theme	AMEP-1	511:516	arg1	mode					498:501	The linking mode	486:501	The linking mode of both AMEP-1 and AMEP-2	486:527	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	2	16	theme	monosaccharide	370:383	arg1	compositions					385:396	their monosaccharide compositions	364:396	their monosaccharide compositions	364:396	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	0	17	dep	Roberts	79:85	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	0	17	dep	Roberts	79:85	arg1	Activities					25:34	Anti-Tumor Activities	14:34	Anti-Tumor Activities	14:34	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	5	18	from	reduction	928:936	arg1	cells					949:953	BGC-823 cells	941:953	BGC-823 cells	941:953	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	5	19	theme	phase	848:852	arg1	reduction					928:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction	842:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells	842:953	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	0	20	theme	Anti-Tumor	14:23	arg1	Activities					25:34	Anti-Tumor Activities	14:34	Anti-Tumor Activities	14:34	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	5	21	theme	reactive	855:862	arg1	species					871:877	reactive oxygen species	855:877	reactive oxygen species production	855:888	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	2	22	theme	polysaccharide	279:292	arg1	AMEP-1					294:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	5	23	theme	potential	918:926	arg1	reduction					928:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction	842:936	the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells	842:953	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	0	24	theme	Exopolysaccharides	39:56	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	0	24	theme	Exopolysaccharides	39:56	arg1	Activities					25:34	Anti-Tumor Activities	14:34	Anti-Tumor Activities	14:34	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	5	25	theme	mitochondrial	895:907	arg1	potential					918:926	mitochondrial membrane potential	895:926	mitochondrial membrane potential	895:926	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	4	26	theme	concentration-dependent	695:717	arg1	manner					719:724	a time- and concentration-dependent manner	683:724	a time- and concentration-dependent manner	683:724	AMEP-2 inhibited the proliferation of BGC-823 cells in a time- and concentration-dependent manner.
30959773	3	27	theme	AMEP-2	522:527	arg1	Manp-					532:536	Manp-	532:536	Manp-(1→4)	532:541	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	3	27	theme	AMEP-2	522:527	arg1	mode					498:501	The linking mode	486:501	The linking mode of both AMEP-1 and AMEP-2	486:527	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	1	28	theme	extracellular	106:118	arg1	polysaccharide					120:133	an extracellular polysaccharide	103:133	an extracellular polysaccharide from Alternaria mali Roberts (AMEP)	103:169	In this study, an extracellular polysaccharide from Alternaria mali Roberts (AMEP) was extracted, and its structure was characterized, in addition to its antitumor activity in vitro.
30959773	3	29	theme	branched	567:574	arg1	chains					576:581	the branched chains	563:581	the branched chains	563:581	The linking mode of both AMEP-1 and AMEP-2 is Manp-(1→4) and Glcp-(1→6), and the branched chains are connected to the main chain through O-6.
30959773	5	30	theme	BGC-823	941:947	arg1	cells					949:953	BGC-823 cells	941:953	BGC-823 cells	941:953	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	6	31	theme	novel	1019:1023	arg1	agent					1036:1040	a novel anti-tumor agent	1017:1040	a novel anti-tumor agent	1017:1040	Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.
30959773	6	31	theme	novel	1019:1023	arg1	potential					980:988	potential	980:988	potential for further development	980:1012	Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.
30959773	1	32	dep	activity	252:259	arg1	addition					226:233	addition	226:233	addition	226:233	In this study, an extracellular polysaccharide from Alternaria mali Roberts (AMEP) was extracted, and its structure was characterized, in addition to its antitumor activity in vitro.
30959773	5	33	theme	species	871:877	arg1	production					879:888	reactive oxygen species production	855:888	reactive oxygen species production	855:888	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	5	34	theme	membrane	909:916	arg1	potential					918:926	mitochondrial membrane potential	895:926	mitochondrial membrane potential	895:926	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	5	35	theme	cell	821:824	arg1	arrest					832:837	cell cycle arrest	821:837	cell cycle arrest	821:837	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	5	36	theme	oxygen	864:869	arg1	species					871:877	reactive oxygen species	855:877	reactive oxygen species production	855:888	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	2	37	theme	Neutral	271:277	arg1	AMEP-1					294:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1	271:299	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	2	38	theme	polysaccharide	313:326	arg1	AMEP-2					328:333	anionic polysaccharide AMEP-2	305:333	anionic polysaccharide AMEP-2	305:333	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30959773	6	39	theme	anti-tumor	1025:1034	arg1	agent					1036:1040	a novel anti-tumor agent	1017:1040	a novel anti-tumor agent	1017:1040	Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.
30959773	6	39	theme	anti-tumor	1025:1034	arg1	potential					980:988	potential	980:988	potential for further development	980:1012	Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.
30959773	5	40	theme	S	846:846	arg1	phase					848:852	S phase	846:852	S phase	846:852	AMEP-2 also induced the apoptosis of BGC-823 cells, and showed anti-tumor effects by inducing cell cycle arrest in the S phase, reactive oxygen species production, and mitochondrial membrane potential reduction in BGC-823 cells.
30959773	0	41	from	Activities	25:34	arg1	Alternaria					63:72	Alternaria	63:72	Alternaria	63:72	Structure and Anti-Tumor Activities of Exopolysaccharides from Alternaria mali Roberts.
30959773	4	42	theme	time-	685:689	arg1	manner					719:724	a time- and concentration-dependent manner	683:724	a time- and concentration-dependent manner	683:724	AMEP-2 inhibited the proliferation of BGC-823 cells in a time- and concentration-dependent manner.
30959773	6	43	theme	further	994:1000	arg1	development					1002:1012	further development	994:1012	further development	994:1012	Therefore, AMEP-2 shows potential for further development as a novel anti-tumor agent.
30959773	2	44	theme	different	468:476	arg1	ratios					478:483	different ratios	468:483	different ratios	468:483	Neutral polysaccharide AMEP-1 and anionic polysaccharide AMEP-2 were isolated from AMEP, and their monosaccharide compositions consisted of mannose (Man), glucose (Glc), and galactose (Gal) but at different ratios.
30854239	0	0	theme	Early-Onset	85:95	arg1	Preeclampsia					104:115	Early-Onset Severe Preeclampsia	85:115	Early-Onset Severe Preeclampsia	85:115	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	5	1	theme	severe	670:675	arg1	preeclampsia					677:688	severe preeclampsia	670:688	severe preeclampsia	670:688	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	5	2	theme	Lectin	553:558	arg1	analysis					565:572	Lectin blot analysis	553:572	Lectin blot analysis	553:572	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	2	3	dep	METHODS	301:307	arg1	METHODS					301:307	METHODS TfR1 and HIF-1α	301:323	METHODS TfR1 and HIF-1α	301:323	METHODS TfR1 and HIF-1α were detected by western blot.
30854239	2	3	dep	METHODS	301:307	arg1	HIF-1α					318:323	HIF-1α	318:323	HIF-1α	318:323	METHODS TfR1 and HIF-1α were detected by western blot.
30854239	2	3	dep	METHODS	301:307	arg1	TfR1					309:312	TfR1	309:312	TfR1	309:312	METHODS TfR1 and HIF-1α were detected by western blot.
30854239	1	4	theme	women	260:264	arg1	placentas					247:255	placentas	247:255	placentas of women with IDAP and severe preeclampsia	247:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	0	5	from	Profile	14:20	arg1	Preeclampsia					104:115	Early-Onset Severe Preeclampsia	85:115	Early-Onset Severe Preeclampsia	85:115	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	0	5	from	Profile	14:20	arg1	Deficiency					70:79	Gestational Iron Deficiency	53:79	Gestational Iron Deficiency	53:79	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	5	6	theme	β1-4	617:620	arg1	N-acetylglucosamine					622:640	galactose β1-4 N-acetylglucosamine	607:640	galactose β1-4 N-acetylglucosamine (Gal-GlcNAc)	607:653	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	5	6	theme	β1-4	617:620	arg1	Gal-GlcNAc					643:652	Gal-GlcNAc	643:652	Gal-GlcNAc	643:652	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	7	7	theme	TfR1	955:958	arg1	expression					931:940	the low expression	923:940	the low expression of placental TfR1 in women with preeclampsia	923:985	These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
30854239	5	8	theme	N-acetylglucosamine	622:640	arg1	overexpression					589:602	an overexpression	586:602	an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia	586:688	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	0	9	theme	Severe	97:102	arg1	Preeclampsia					104:115	Early-Onset Severe Preeclampsia	85:115	Early-Onset Severe Preeclampsia	85:115	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	1	10	theme	factor-1α	175:183	arg1	expression					143:152	the expression	139:152	the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia	139:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	4	11	dep	RESULTS	468:474	arg1	was					482:484	was	482:484	was no difference in the expression of TfR1 and HIF-1α between groups	482:550	RESULTS There was no difference in the expression of TfR1 and HIF-1α between groups.
30854239	6	12	theme	TfR1	817:820	arg1	glycans					806:812	the mannose glycans	794:812	the mannose glycans of TfR1	794:820	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	5	13	theme	mannose	659:665	arg1	overexpression					589:602	an overexpression	586:602	an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia	586:688	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	1	14	from	expression	143:152	arg1	placentas					247:255	placentas	247:255	placentas of women with IDAP and severe preeclampsia	247:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	6	15	from	increase	706:713	arg1	Gal-GlcNAc					718:727	Gal-GlcNAc	718:727	Gal-GlcNAc	718:727	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	3	16	theme	specific	443:450	arg1	binding					459:465	specific lectin binding	443:465	specific lectin binding	443:465	Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.
30854239	0	17	theme	Glycosylation	0:12	arg1	Profile					14:20	Glycosylation Profile	0:20	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.	0:116	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	1	18	theme	severe	280:285	arg1	preeclampsia					287:298	severe preeclampsia	280:298	severe preeclampsia	280:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	4	19	from	difference	489:498	arg1	expression					507:516	the expression	503:516	the expression of TfR1 and HIF-1α	503:535	RESULTS There was no difference in the expression of TfR1 and HIF-1α between groups.
30854239	7	20	theme	placental	945:953	arg1	TfR1					955:958	placental TfR1	945:958	placental TfR1	945:958	These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
30854239	3	21	theme	lectin	452:457	arg1	binding					459:465	specific lectin binding	443:465	specific lectin binding	443:465	Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.
30854239	5	22	theme	galactose	607:615	arg1	N-acetylglucosamine					622:640	galactose β1-4 N-acetylglucosamine	607:640	galactose β1-4 N-acetylglucosamine (Gal-GlcNAc)	607:653	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	5	22	theme	galactose	607:615	arg1	Gal-GlcNAc					643:652	Gal-GlcNAc	643:652	Gal-GlcNAc	643:652	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	7	23	from	expression	931:940	arg1	women					963:967	women	963:967	women with preeclampsia	963:985	These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
30854239	6	24	theme	structures	779:788	arg1	presence					757:764	the increased presence	743:764	the increased presence of antennary structures	743:788	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	0	25	theme	Receptor	41:48	arg1	Profile					14:20	Glycosylation Profile	0:20	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.	0:116	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	7	26	with	women	963:967	arg1	preeclampsia					974:985	preeclampsia	974:985	preeclampsia	974:985	These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
30854239	1	27	theme	TfR1	195:198	arg1	expression					143:152	the expression	139:152	the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia	139:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	1	28	theme	TfR1-attached	205:217	arg1	monosaccharides					228:242	TfR1-attached terminal monosaccharides	205:242	TfR1-attached terminal monosaccharides	205:242	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	0	29	theme	Transferrin	29:39	arg1	Receptor					41:48	the Transferrin Receptor	25:48	the Transferrin Receptor	25:48	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	4	30	theme	HIF-1α	530:535	arg1	expression					507:516	the expression	503:516	the expression of TfR1 and HIF-1α	503:535	RESULTS There was no difference in the expression of TfR1 and HIF-1α between groups.
30854239	1	31	theme	terminal	219:226	arg1	monosaccharides					228:242	TfR1-attached terminal monosaccharides	205:242	TfR1-attached terminal monosaccharides	205:242	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	0	32	theme	Gestational	53:63	arg1	Deficiency					70:79	Gestational Iron Deficiency	53:79	Gestational Iron Deficiency	53:79	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	6	33	theme	proteins	875:882	arg1	presence					839:846	the presence	835:846	the presence of misfolded or incomplete proteins	835:882	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	3	34	theme	terminal	415:422	arg1	monosaccharides					424:438	the terminal monosaccharides	411:438	the terminal monosaccharides	411:438	Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.
30854239	6	35	theme	incomplete	864:873	arg1	proteins					875:882	misfolded or incomplete proteins	851:882	misfolded or incomplete proteins	851:882	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	1	36	with	women	260:264	arg1	IDAP					271:274	IDAP	271:274	IDAP	271:274	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	1	36	with	women	260:264	arg1	preeclampsia					287:298	severe preeclampsia	280:298	severe preeclampsia	280:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	2	37	theme	western	342:348	arg1	blot					350:353	western blot	342:353	western blot	342:353	METHODS TfR1 and HIF-1α were detected by western blot.
30854239	6	38	theme	mannose	798:804	arg1	glycans					806:812	the mannose glycans	794:812	the mannose glycans of TfR1	794:820	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	6	39	theme	antennary	769:777	arg1	structures					779:788	antennary structures	769:788	antennary structures	769:788	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	6	40	dep	CONCLUSION	691:700	arg1	indicate					826:833	indicate	826:833	may indicate the presence of misfolded or incomplete proteins	822:882	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	6	40	dep	CONCLUSION	691:700	arg1	due					736:738	due	736:738	due	736:738	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	6	40	dep	CONCLUSION	691:700	arg1	increase					706:713	The increase	702:713	The increase in Gal-GlcNAc	702:727	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	1	41	theme	monosaccharides	228:242	arg1	expression					143:152	the expression	139:152	the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia	139:298	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	6	42	theme	misfolded	851:859	arg1	proteins					875:882	misfolded or incomplete proteins	851:882	misfolded or incomplete proteins	851:882	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	5	43	theme	blot	560:563	arg1	analysis					565:572	Lectin blot analysis	553:572	Lectin blot analysis	553:572	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	5	44	from	overexpression	589:602	arg1	preeclampsia					677:688	severe preeclampsia	670:688	severe preeclampsia	670:688	Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
30854239	7	45	theme	low	927:929	arg1	expression					931:940	the low expression	923:940	the low expression of placental TfR1 in women with preeclampsia	923:985	These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
30854239	0	46	theme	Iron	65:68	arg1	Deficiency					70:79	Gestational Iron Deficiency	53:79	Gestational Iron Deficiency	53:79	Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
30854239	4	47	theme	TfR1	521:524	arg1	expression					507:516	the expression	503:516	the expression of TfR1 and HIF-1α	503:535	RESULTS There was no difference in the expression of TfR1 and HIF-1α between groups.
30854239	6	48	theme	increased	747:755	arg1	presence					757:764	the increased presence	743:764	the increased presence of antennary structures	743:788	CONCLUSION The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins.
30854239	1	49	theme	hypoxia-inducible	157:173	arg1	HIF-1α					186:191	HIF-1α	186:191	HIF-1α	186:191	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	1	49	theme	hypoxia-inducible	157:173	arg1	factor-1α					175:183	hypoxia-inducible factor-1α	157:183	hypoxia-inducible factor-1α (HIF-1α)	157:192	OBJECTIVE To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
30854239	3	50	theme	TfR1	376:379	arg1	Immunoadsorption					356:371	Immunoadsorption	356:371	Immunoadsorption of TfR1	356:379	Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.
30831166	4	0	theme	change	852:857	arg1	40.1 °C					837:843	40.1 °C	837:843	40.1 °C	837:843	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	0	theme	change	852:857	arg1	fiber					859:863	phase change fiber	846:863	phase change fiber	846:863	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	1	theme	polyethylene	961:972	arg1	FK-g-APEG					982:990	FK-g-APEG	982:990	FK-g-APEG	982:990	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	1	theme	polyethylene	961:972	arg1	glycol					974:979	feather keratin-g-allyloxy polyethylene glycol	934:979	feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG)	934:991	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	2	theme	phase	846:850	arg1	40.1 °C					837:843	40.1 °C	837:843	40.1 °C	837:843	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	2	theme	phase	846:850	arg1	fiber					859:863	phase change fiber	846:863	phase change fiber	846:863	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	3	3	from	δ = 155.6 ppm	541:553	arg1	shifts					531:536	chemical shifts	522:536	chemical shifts at δ = 155.6 ppm	522:553	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	1	4	theme	first	286:290	arg1	time					292:295	the first time	282:295	the first time	282:295	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	3	5	theme	feather	711:717	arg1	keratin					719:725	feather keratin	711:725	feather keratin (FK)	711:730	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	3	5	theme	feather	711:717	arg1	FK					728:729	FK	728:729	FK	728:729	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	1	6	theme	novel	112:116	arg1	SA/FK-g-APEG					182:193	SA/FK-g-APEG	182:193	SA/FK-g-APEG	182:193	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	6	theme	novel	112:116	arg1	glycol					174:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol	108:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	3	7	theme	NMR	488:490	arg1	result					492:497	The NMR result	484:497	The NMR result	484:497	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	4	8	from	decline	757:763	arg1	peak					784:787	the endothermic peak	768:787	the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber)	768:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	2	9	theme	chemical	302:309	arg1	structure					311:319	The chemical structure	298:319	The chemical structure of the composite fiber	298:342	The chemical structure of the composite fiber was characterized by FT-IF and NMR, the thermal property was characterized by DSC, and the morphology features was analyzed by SEM and EDS.
30831166	1	10	theme	composite	196:204	arg1	fiber					219:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	4	11	dep	40.1 °C	837:843	arg1	to					834:835	to	834:835	to	834:835	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	1	12	theme	phase	206:210	arg1	fiber					219:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	0	13	theme	alginate/feather	7:22	arg1	glycol					56:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	5	14	theme	optimal	1087:1093	arg1	speed					1107:1111	the optimal Centrifugal speed	1083:1111	the optimal Centrifugal speed	1083:1111	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	5	14	theme	optimal	1087:1093	arg1	500 r/min					1116:1124	500 r/min	1116:1124	500 r/min	1116:1124	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	4	15	from	40.1 °C	837:843	arg1	melting					800:806	melting	800:806	melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber)	800:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	15	from	40.1 °C	837:843	arg1	APEG					815:818	the APEG	811:818	the APEG from 54.87 °C to 40.1 °C (phase change fiber)	811:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	1	16	theme	change	212:217	arg1	fiber					219:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	0	17	theme	Sodium	0:5	arg1	glycol					56:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	2	18	theme	fiber	338:342	arg1	structure					311:319	The chemical structure	298:319	The chemical structure of the composite fiber	298:342	The chemical structure of the composite fiber was characterized by FT-IF and NMR, the thermal property was characterized by DSC, and the morphology features was analyzed by SEM and EDS.
30831166	0	19	theme	polyethylene	43:54	arg1	glycol					56:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	4	20	theme	DSC	737:739	arg1	results					741:747	The DSC results	733:747	The DSC results	733:747	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	2	21	theme	composite	328:336	arg1	fiber					338:342	the composite fiber	324:342	the composite fiber	324:342	The chemical structure of the composite fiber was characterized by FT-IF and NMR, the thermal property was characterized by DSC, and the morphology features was analyzed by SEM and EDS.
30831166	2	22	theme	morphology	435:444	arg1	features					446:453	the morphology features	431:453	the morphology features	431:453	The chemical structure of the composite fiber was characterized by FT-IF and NMR, the thermal property was characterized by DSC, and the morphology features was analyzed by SEM and EDS.
30831166	4	23	theme	endothermic	772:782	arg1	peak					784:787	the endothermic peak	768:787	the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber)	768:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	5	24	theme	spinning	1048:1055	arg1	temperature					1057:1067	the optimal spinning temperature	1036:1067	the optimal spinning temperature	1036:1067	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	5	24	theme	spinning	1048:1055	arg1	40 °C					1072:1076	40 °C	1072:1076	40 °C	1072:1076	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	0	25	dep	fiber	86:90	arg1	glycol					56:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	2	26	theme	thermal	384:390	arg1	property					392:399	the thermal property	380:399	the thermal property	380:399	The chemical structure of the composite fiber was characterized by FT-IF and NMR, the thermal property was characterized by DSC, and the morphology features was analyzed by SEM and EDS.
30831166	0	27	theme	composite	63:71	arg1	fiber					86:90	composite phase change fiber	63:90	composite phase change fiber	63:90	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	4	28	theme	sodium	909:914	arg1	SA					926:927	SA	926:927	SA	926:927	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	28	theme	sodium	909:914	arg1	alginate					916:923	sodium alginate	909:923	sodium alginate (SA)	909:928	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	3	29	theme	polyethylene	662:673	arg1	APEG					683:686	APEG	683:686	APEG	683:686	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	3	29	theme	polyethylene	662:673	arg1	glycol					675:680	allyloxy polyethylene glycol	653:680	allyloxy polyethylene glycol (APEG)	653:687	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	5	30	theme	mechanical	998:1007	arg1	properties					1009:1018	The mechanical properties	994:1018	The mechanical properties test	994:1023	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	0	31	theme	change	79:84	arg1	fiber					86:90	composite phase change fiber	63:90	composite phase change fiber	63:90	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	5	32	theme	properties	1009:1018	arg1	test					1020:1023	The mechanical properties test	994:1023	The mechanical properties test	994:1023	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	1	33	theme	sodium	118:123	arg1	SA/FK-g-APEG					182:193	SA/FK-g-APEG	182:193	SA/FK-g-APEG	182:193	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	33	theme	sodium	118:123	arg1	glycol					174:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol	108:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	0	34	theme	phase	73:77	arg1	fiber					86:90	composite phase change fiber	63:90	composite phase change fiber	63:90	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	4	35	theme	APEG	815:818	arg1	melting					800:806	melting	800:806	melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber)	800:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	1	36	theme	alginate/feather	125:140	arg1	SA/FK-g-APEG					182:193	SA/FK-g-APEG	182:193	SA/FK-g-APEG	182:193	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	36	theme	alginate/feather	125:140	arg1	glycol					174:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol	108:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	4	37	theme	strong	882:887	arg1	interaction					889:899	the strong interaction	878:899	the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG)	878:991	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	38	theme	related	789:795	arg1	peak					784:787	the endothermic peak	768:787	the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber)	768:864	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	1	39	theme	keratin-g-allyloxy	142:159	arg1	SA/FK-g-APEG					182:193	SA/FK-g-APEG	182:193	SA/FK-g-APEG	182:193	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	39	theme	keratin-g-allyloxy	142:159	arg1	glycol					174:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol	108:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	3	40	theme	allyloxy	653:660	arg1	APEG					683:686	APEG	683:686	APEG	683:686	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	3	40	theme	allyloxy	653:660	arg1	glycol					675:680	allyloxy polyethylene glycol	653:680	allyloxy polyethylene glycol (APEG)	653:687	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
30831166	4	41	from	melting	800:806	arg1	40.1 °C					837:843	40.1 °C	837:843	40.1 °C	837:843	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	41	from	melting	800:806	arg1	fiber					859:863	phase change fiber	846:863	phase change fiber	846:863	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	1	42	theme	polyethylene	161:172	arg1	SA/FK-g-APEG					182:193	SA/FK-g-APEG	182:193	SA/FK-g-APEG	182:193	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	42	theme	polyethylene	161:172	arg1	glycol					174:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol	108:179	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	1	43	theme	centrifugal	257:267	arg1	spinning					269:276	centrifugal spinning	257:276	centrifugal spinning	257:276	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	4	44	theme	keratin-g-allyloxy	942:959	arg1	FK-g-APEG					982:990	FK-g-APEG	982:990	FK-g-APEG	982:990	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	44	theme	keratin-g-allyloxy	942:959	arg1	glycol					974:979	feather keratin-g-allyloxy polyethylene glycol	934:979	feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG)	934:991	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	5	45	theme	optimal	1040:1046	arg1	temperature					1057:1067	the optimal spinning temperature	1036:1067	the optimal spinning temperature	1036:1067	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	5	45	theme	optimal	1040:1046	arg1	40 °C					1072:1076	40 °C	1072:1076	40 °C	1072:1076	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	1	46	theme	glycol	174:179	arg1	fiber					219:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber	108:223	In this study, the novel sodium alginate/feather keratin-g-allyloxy polyethylene glycol (SA/FK-g-APEG) composite phase change fiber was designed and fabricated via centrifugal spinning for the first time.
30831166	4	47	theme	feather	934:940	arg1	FK-g-APEG					982:990	FK-g-APEG	982:990	FK-g-APEG	982:990	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	4	47	theme	feather	934:940	arg1	glycol					974:979	feather keratin-g-allyloxy polyethylene glycol	934:979	feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG)	934:991	The DSC results show an decline in the endothermic peak related to melting of the APEG from 54.87 °C to 40.1 °C (phase change fiber), indicating the strong interaction between sodium alginate (SA) and feather keratin-g-allyloxy polyethylene glycol (FK-g-APEG).
30831166	5	48	theme	Centrifugal	1095:1105	arg1	speed					1107:1111	the optimal Centrifugal speed	1083:1111	the optimal Centrifugal speed	1083:1111	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	5	48	theme	Centrifugal	1095:1105	arg1	500 r/min					1116:1124	500 r/min	1116:1124	500 r/min	1116:1124	The mechanical properties test shows that the optimal spinning temperature is 40 °C, and the optimal Centrifugal speed is 500 r/min.
30831166	0	49	theme	keratin-g-allyloxy	24:41	arg1	glycol					56:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol	0:61	Sodium alginate/feather keratin-g-allyloxy polyethylene glycol composite phase change fiber.
30831166	3	50	theme	chemical	522:529	arg1	shifts					531:536	chemical shifts	522:536	chemical shifts at δ = 155.6 ppm	522:553	The NMR result demonstrates there are chemical shifts at δ = 155.6 ppm indicating CC has been successfully introduced via acylation，and at δ = 70.06 ppm indicating that allyloxy polyethylene glycol (APEG) has been grafted onto feather keratin (FK).
31838069	0	0	theme	gingival	83:90	arg1	film					111:114	gingival mucoadhesive patch film	83:114	gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract	83:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	3	1	theme	mucoadhesive	662:673	arg1	time					675:678	mucoadhesive time	662:678	mucoadhesive time	662:678	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	0	2	theme	extract	167:173	arg1	delivery					120:127	delivery	120:127	delivery of gambier (Uncaria gambir Roxb) leaf extract	120:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	5	3	theme	patch	1032:1036	arg1	pH					1022:1023	surface pH	1014:1023	surface pH of the patch	1014:1036	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	6	4	theme	leaf	1217:1220	arg1	extract					1222:1228	the gambier leaf extract	1205:1228	the gambier leaf extract	1205:1228	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	6	5	theme	FT-IR	1262:1266	arg1	characterization					1268:1283	the FT-IR characterization	1258:1283	the FT-IR characterization	1258:1283	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	1	6	theme	tapioca	309:315	arg1	TS					325:326	TS	325:326	TS	325:326	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	1	6	theme	tapioca	309:315	arg1	starch					317:322	tapioca starch	309:322	tapioca starch (TS) composite	309:337	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	0	7	theme	leaf	162:165	arg1	extract					167:173	gambier (Uncaria gambir Roxb) leaf extract	132:173	gambier (Uncaria gambir Roxb) leaf extract	132:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	1	8	theme	mucoadhesive	235:246	arg1	patch					255:259	the mucoadhesive buccal patch	231:259	the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes	231:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	0	9	theme	patch	105:109	arg1	film					111:114	gingival mucoadhesive patch film	83:114	gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract	83:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	1	10	theme	starch	317:322	arg1	chitosan					291:298	chitosan	291:298	chitosan (CH)	291:303	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	1	10	theme	starch	317:322	arg1	complexes					354:362	the polymer complexes	342:362	the polymer complexes	342:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	1	10	theme	starch	317:322	arg1	composite					329:337	tapioca starch (TS) composite	309:337	tapioca starch (TS) composite	309:337	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	5	11	theme	thickness	950:958	arg1	uniformity					960:969	the mass and thickness uniformity	937:969	the mass and thickness uniformity	937:969	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	5	12	theme	patch	888:892	arg1	characterization					856:871	The characterization	852:871	The characterization of the optimum patch	852:892	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	5	13	theme	optimum	880:886	arg1	patch					888:892	the optimum patch	876:892	the optimum patch	876:892	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	1	14	theme	buccal	248:253	arg1	patch					255:259	the mucoadhesive buccal patch	231:259	the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes	231:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	2	15	theme	CH	469:470	arg1	composition					454:464	the composition	450:464	the composition of CH and TS	450:477	The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS.
31838069	5	16	dep	times	1068:1072	arg1	folding					1045:1051	6, folding endurance >300 times	1042:1072	folding	1045:1051	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	3	17	theme	physical	484:491	arg1	endurance					596:604	folding endurance	588:604	folding endurance	588:604	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	characteristics					506:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	index					628:632	swelling index	619:632	swelling index	619:632	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	mass					557:560	mass	557:560	mass	557:560	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	percent					635:641	percent	635:641	percent of elongation	635:655	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	time					675:678	mucoadhesive time	662:678	mucoadhesive time	662:678	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	thickness					566:574	thickness	566:574	thickness	566:574	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	17	theme	physical	484:491	arg1	pH					615:616	surface pH	607:616	surface pH	607:616	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	5	18	theme	mucoadhesive	1120:1131	arg1	time					1133:1136	mucoadhesive time	1120:1136	mucoadhesive time	1120:1136	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	2	19	dep	designed	391:398	arg1	based					400:404	based	400:404	based on 22 factorial design in order to optimize the composition of CH and TS	400:477	The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS.
31838069	3	20	dep	mass	557:560	arg1	uniformity					576:585	uniformity	576:585	uniformity	576:585	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	0	21	theme	film	111:114	arg1	formulation					68:78	the formulation	64:78	the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract	64:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	6	22	theme	patch	1348:1352	arg1	component					1354:1362	each patch component	1343:1362	each patch component	1343:1362	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	3	23	theme	elongation	646:655	arg1	endurance					596:604	folding endurance	588:604	folding endurance	588:604	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	index					628:632	swelling index	619:632	swelling index	619:632	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	mass					557:560	mass	557:560	mass	557:560	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	percent					635:641	percent	635:641	percent of elongation	635:655	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	time					675:678	mucoadhesive time	662:678	mucoadhesive time	662:678	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	thickness					566:574	thickness	566:574	thickness	566:574	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	23	theme	elongation	646:655	arg1	pH					615:616	surface pH	607:616	surface pH	607:616	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	6	24	theme	catechin	1164:1171	arg1	content					1173:1179	The catechin content	1160:1179	The catechin content	1160:1179	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	6	24	theme	catechin	1164:1171	arg1	%					1251:1251	92.1667 ± 0.3626%	1235:1251	92.1667 ± 0.3626%	1235:1251	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	6	25	theme	gambier	1209:1215	arg1	extract					1222:1228	the gambier leaf extract	1205:1228	the gambier leaf extract	1205:1228	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	5	26	dep	mass	941:944	arg1	the					937:939	the	937:939	the	937:939	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	3	27	theme	swelling	619:626	arg1	index					628:632	swelling index	619:632	swelling index	619:632	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	5	28	theme	elongation	1075:1084	arg1	percent					1086:1092	elongation percent	1075:1092	elongation percent	1075:1092	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	4	29	theme	TS	776:777	arg1	concentration					752:764	the optimum concentration	740:764	the optimum concentration of CH and TS	740:777	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	4	29	theme	TS	776:777	arg1	900 mg					783:788	900 mg	783:788	900 mg	783:788	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	6	30	theme	active	1189:1194	arg1	agent					1196:1200	the active agent	1185:1200	the active agent of the gambier leaf extract	1185:1228	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	0	31	theme	starch	33:38	arg1	Optimization					0:11	Optimization	0:11	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.	0:174	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	1	32	theme	gambier	264:270	arg1	extract					277:283	gambier leaf extract	264:283	gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes	264:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	4	33	theme	0.968	845:849	arg1	percent					834:840	desirability percent	821:840	desirability percent of 0.968	821:849	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	5	34	theme	uniformity	960:969	arg1	%					990:990	0.4805 ± 0.1887%	975:990	0.4805 ± 0.1887%	975:990	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	5	34	theme	uniformity	960:969	arg1	coefficient					922:932	the variability coefficient	906:932	the variability coefficient of the mass and thickness uniformity	906:969	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	5	35	theme	mass	941:944	arg1	uniformity					960:969	the mass and thickness uniformity	937:969	the mass and thickness uniformity	937:969	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	1	36	theme	present	180:186	arg1	study					188:192	The present study	176:192	The present study	176:192	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	0	37	theme	chitosan-tapioca	16:31	arg1	starch					33:38	chitosan-tapioca starch	16:38	chitosan-tapioca starch composite as polymer	16:59	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	1	38	theme	leaf	272:275	arg1	extract					277:283	gambier leaf extract	264:283	gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes	264:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	3	39	theme	folding	588:594	arg1	endurance					596:604	folding endurance	588:604	folding endurance	588:604	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	endurance					596:604	folding endurance	588:604	folding endurance	588:604	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	characteristics					506:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	index					628:632	swelling index	619:632	swelling index	619:632	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	mass					557:560	mass	557:560	mass	557:560	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	percent					635:641	percent	635:641	percent of elongation	635:655	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	time					675:678	mucoadhesive time	662:678	mucoadhesive time	662:678	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	thickness					566:574	thickness	566:574	thickness	566:574	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	40	theme	chemical	497:504	arg1	pH					615:616	surface pH	607:616	surface pH	607:616	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	2	41	theme	patch	369:373	arg1	formulation					375:385	The patch formulation	365:385	The patch formulation	365:385	The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS.
31838069	6	42	theme	chemical	1313:1320	arg1	interactions					1322:1333	no chemical interactions	1310:1333	no chemical interactions between each patch component	1310:1362	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	1	43	theme	extract	277:283	arg1	patch					255:259	the mucoadhesive buccal patch	231:259	the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes	231:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	3	44	theme	patches	538:544	arg1	endurance					596:604	folding endurance	588:604	folding endurance	588:604	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	characteristics					506:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics	480:520	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	index					628:632	swelling index	619:632	swelling index	619:632	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	mass					557:560	mass	557:560	mass	557:560	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	percent					635:641	percent	635:641	percent of elongation	635:655	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	time					675:678	mucoadhesive time	662:678	mucoadhesive time	662:678	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	thickness					566:574	thickness	566:574	thickness	566:574	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	3	44	theme	patches	538:544	arg1	pH					615:616	surface pH	607:616	surface pH	607:616	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	5	45	theme	endurance	1053:1061	arg1	times					1068:1072	6, folding endurance >300 times	1042:1072	times	1068:1072	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	0	46	theme	gambier	132:138	arg1	extract					167:173	gambier (Uncaria gambir Roxb) leaf extract	132:173	gambier (Uncaria gambir Roxb) leaf extract	132:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	1	47	theme	polymer	346:352	arg1	chitosan					291:298	chitosan	291:298	chitosan (CH)	291:303	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	1	47	theme	polymer	346:352	arg1	complexes					354:362	the polymer complexes	342:362	the polymer complexes	342:362	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	1	47	theme	polymer	346:352	arg1	composite					329:337	tapioca starch (TS) composite	309:337	tapioca starch (TS) composite	309:337	The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes.
31838069	4	48	theme	optimum	744:750	arg1	concentration					752:764	the optimum concentration	740:764	the optimum concentration of CH and TS	740:777	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	4	48	theme	optimum	744:750	arg1	900 mg					783:788	900 mg	783:788	900 mg	783:788	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	2	49	theme	TS	476:477	arg1	composition					454:464	the composition	450:464	the composition of CH and TS	450:477	The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS.
31838069	2	50	theme	factorial	412:420	arg1	design					422:427	22 factorial design	409:427	22 factorial design	409:427	The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS.
31838069	0	51	theme	gambir	149:154	arg1	gambier					132:138	gambier	132:138	gambier (Uncaria gambir Roxb) leaf extract	132:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	0	51	theme	gambir	149:154	arg1	Roxb					156:159	Uncaria gambir Roxb	141:159	Uncaria gambir Roxb	141:159	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	5	52	theme	surface	1014:1020	arg1	pH					1022:1023	surface pH	1014:1023	surface pH of the patch	1014:1036	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	4	53	theme	CH	769:770	arg1	concentration					752:764	the optimum concentration	740:764	the optimum concentration of CH and TS	740:777	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	4	53	theme	CH	769:770	arg1	900 mg					783:788	900 mg	783:788	900 mg	783:788	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	0	54	from	Optimization	0:11	arg1	formulation					68:78	the formulation	64:78	the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract	64:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	0	55	theme	Uncaria	141:147	arg1	gambier					132:138	gambier	132:138	gambier (Uncaria gambir Roxb) leaf extract	132:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	0	55	theme	Uncaria	141:147	arg1	Roxb					156:159	Uncaria gambir Roxb	141:159	Uncaria gambir Roxb	141:159	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
31838069	3	56	theme	prepared	529:536	arg1	patches					538:544	the prepared patches	525:544	the prepared patches	525:544	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	5	57	theme	variability	910:920	arg1	%					990:990	0.4805 ± 0.1887%	975:990	0.4805 ± 0.1887%	975:990	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	5	57	theme	variability	910:920	arg1	coefficient					922:932	the variability coefficient	906:932	the variability coefficient of the mass and thickness uniformity	906:969	The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min.
31838069	6	58	theme	extract	1222:1228	arg1	agent					1196:1200	the active agent	1185:1200	the active agent of the gambier leaf extract	1185:1228	The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
31838069	4	59	theme	desirability	821:832	arg1	percent					834:840	desirability percent	821:840	desirability percent of 0.968	821:849	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	4	60	theme	statistical	718:728	arg1	analysis					730:737	statistical analysis	718:737	statistical analysis	718:737	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	4	61	with	300 mg	794:799	arg1	percent					834:840	desirability percent	821:840	desirability percent of 0.968	821:849	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	3	62	theme	surface	607:613	arg1	pH					615:616	surface pH	607:616	surface pH	607:616	The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated.
31838069	4	63	with	900 mg	783:788	arg1	percent					834:840	desirability percent	821:840	desirability percent of 0.968	821:849	Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968.
31838069	0	64	theme	mucoadhesive	92:103	arg1	film					111:114	gingival mucoadhesive patch film	83:114	gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract	83:173	Optimization of chitosan-tapioca starch composite as polymer in the formulation of gingival mucoadhesive patch film for delivery of gambier (Uncaria gambir Roxb) leaf extract.
30212760	0	0	theme	putrescine-dependent	58:77	arg1	alterations					79:89	putrescine-dependent alterations	58:89	putrescine-dependent alterations of cell wall composition	58:114	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	2	1	theme	rice	306:309	arg1	ability					295:301	the ability	291:301	the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd)	291:371	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	4	2	theme	rice	685:688	arg1	roots					690:694	rice roots	685:694	rice roots	685:694	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	7	3	theme	putrescine	1109:1118	arg1	addition					1097:1104	The addition	1093:1104	The addition of putrescine	1093:1118	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	4	4	from	accumulation	771:782	arg1	roots					795:799	roots	795:799	roots	795:799	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	3	5	theme	chlorophyll	433:443	arg1	contents					445:452	chlorophyll contents	433:452	chlorophyll contents	433:452	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	6	from	levels	471:476	arg1	cultivars					486:494	rice cultivars	481:494	rice cultivars Nipponbare (japonica)	481:516	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	6	from	levels	471:476	arg1	japonica					508:515	japonica	508:515	japonica	508:515	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	6	from	levels	471:476	arg1	Kasalath					522:529	Kasalath	522:529	Kasalath (indica)	522:538	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	6	from	levels	471:476	arg1	indica					532:537	indica	532:537	indica	532:537	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	7	dep	cultivars	486:494	arg1	Nipponbare					496:505	Nipponbare	496:505	Nipponbare	496:505	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	4	8	theme	Cd2+	787:790	arg1	accumulation					771:782	the accumulation	767:782	the accumulation of Cd2+ in roots	767:799	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	5	9	theme	OsNramp5	926:933	arg1	expression					901:910	the expression	897:910	the expression of OsHMA2 and OsNramp5 in the root	897:945	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	7	10	theme	OsHMA2	1333:1338	arg1	expression					1319:1328	the expression	1315:1328	the expression of OsHMA2 and OsNramp5 in the root	1315:1363	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	5	11	theme	root-to-shoot	847:859	arg1	translocation					861:873	root-to-shoot translocation	847:873	root-to-shoot translocation	847:873	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	8	12	theme	wall	1524:1527	arg1	capacity					1540:1547	the cell wall Cd-binding capacity	1515:1547	the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels	1515:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	6	13	theme	cultivars	1044:1052	arg1	roots					1025:1029	the roots	1021:1029	the roots of both rice cultivars provided with NH4+ compared with NO3-	1021:1090	Levels of the signaling molecule putrescine were significantly higher in the roots of both rice cultivars provided with NH4+ compared with NO3-.
30212760	5	14	from	expression	901:910	arg1	root					942:945	the root	938:945	the root	938:945	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	8	15	theme	cell	1519:1522	arg1	capacity					1540:1547	the cell wall Cd-binding capacity	1515:1547	the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels	1515:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	0	16	theme	wall	99:102	arg1	composition					104:114	cell wall composition	94:114	cell wall composition	94:114	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	8	17	theme	contents	1596:1603	arg1	processes					1623:1631	processes	1623:1631	processes induced by increasing putrescine levels	1623:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	8	17	theme	contents	1596:1603	arg1	transport					1612:1620	Cd transport	1609:1620	Cd transport	1609:1620	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	8	17	theme	contents	1596:1603	arg1	alterations					1556:1566	alterations	1556:1566	alterations of pectin and hemicellulose contents	1556:1603	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	7	18	theme	PME	1286:1288	arg1	activity					1290:1297	PME activity	1286:1297	PME activity	1286:1297	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	4	19	from	activity	673:680	arg1	roots					690:694	rice roots	685:694	rice roots	685:694	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	5	20	theme	Cd2+	878:881	arg1	absorption					832:841	absorption	832:841	absorption	832:841	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	5	20	theme	Cd2+	878:881	arg1	translocation					861:873	root-to-shoot translocation	847:873	root-to-shoot translocation	847:873	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	8	21	theme	hemicellulose	1582:1594	arg1	contents					1596:1603	pectin and hemicellulose contents	1571:1603	pectin and hemicellulose contents	1571:1603	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	1	22	theme	nitrogen	147:154	arg1	forms					138:142	different forms	128:142	different forms of nitrogen (NO3- or NH4+)	128:169	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	0	23	theme	cell	94:97	arg1	composition					104:114	cell wall composition	94:114	cell wall composition	94:114	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	7	24	theme	OsNramp5	1344:1351	arg1	expression					1319:1328	the expression	1315:1328	the expression of OsHMA2 and OsNramp5 in the root	1315:1363	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	4	25	theme	cell	742:745	arg1	wall					747:750	the cell wall	738:750	the cell wall	738:750	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	2	26	theme	present	237:243	arg1	study					245:249	the present study	233:249	the present study	233:249	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	6	27	theme	putrescine	981:990	arg1	Levels					948:953	Levels	948:953	Levels of the signaling molecule putrescine	948:990	Levels of the signaling molecule putrescine were significantly higher in the roots of both rice cultivars provided with NH4+ compared with NO3-.
30212760	6	28	theme	signaling	962:970	arg1	molecule					972:979	the signaling molecule	958:979	the signaling molecule putrescine	958:990	Levels of the signaling molecule putrescine were significantly higher in the roots of both rice cultivars provided with NH4+ compared with NO3-.
30212760	8	29	theme	noxious	1480:1486	arg1	effects					1488:1494	the noxious effects	1476:1494	the noxious effects of Cd	1476:1500	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	8	30	theme	NH4+	1410:1413	arg1	treatment					1415:1423	NH4+ treatment	1410:1423	NH4+ treatment	1410:1423	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	9	31	from	accumulation	1718:1729	arg1	rice					1734:1737	rice	1734:1737	rice	1734:1737	Our findings suggest methods to decrease Cd accumulation in rice by applying NH4+ fertilizers.
30212760	8	32	theme	putrescine	1655:1664	arg1	levels					1666:1671	putrescine levels	1655:1671	putrescine levels	1655:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	4	33	theme	methylesterase	652:665	arg1	activity					673:680	the pectin methylesterase (PME) activity	641:680	the pectin methylesterase (PME) activity in rice roots	641:694	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	7	34	theme	wall	1235:1238	arg1	pectin					1240:1245	root cell wall pectin	1225:1245	root cell wall pectin	1225:1245	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	3	35	theme	rice	481:484	arg1	cultivars					486:494	rice cultivars	481:494	rice cultivars Nipponbare (japonica)	481:516	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	3	35	theme	rice	481:484	arg1	japonica					508:515	japonica	508:515	japonica	508:515	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	5	36	theme	OsHMA2	915:920	arg1	expression					901:910	the expression	897:910	the expression of OsHMA2 and OsNramp5 in the root	897:945	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	2	37	theme	metal	354:358	arg1	Cd					369:370	Cd	369:370	Cd	369:370	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	2	37	theme	metal	354:358	arg1	cadmium					360:366	the toxic heavy metal cadmium	338:366	the toxic heavy metal cadmium (Cd)	338:371	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	0	38	theme	Cd	19:20	arg1	toxicity					22:29	Cd toxicity	19:29	Cd toxicity in rice	19:37	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	0	39	theme	composition	104:114	arg1	alterations					79:89	putrescine-dependent alterations	58:89	putrescine-dependent alterations of cell wall composition	58:114	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	4	40	theme	hemicellulose	604:616	arg1	contents					618:625	the pectin and hemicellulose contents	589:625	contents	618:625	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	2	41	theme	heavy	348:352	arg1	Cd					369:370	Cd	369:370	Cd	369:370	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	2	41	theme	heavy	348:352	arg1	cadmium					360:366	the toxic heavy metal cadmium	338:366	the toxic heavy metal cadmium (Cd)	338:371	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	7	42	theme	chlorophyll	1183:1193	arg1	content					1195:1201	the chlorophyll content	1179:1201	the chlorophyll content in shoots	1179:1211	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	0	43	dep	Oryza	40:44	arg1	sativa					46:51	Oryza sativa	40:51	Oryza sativa	40:51	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	8	44	theme	due	1549:1551	arg1	capacity					1540:1547	the cell wall Cd-binding capacity	1515:1547	the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels	1515:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	2	45	theme	toxic	342:346	arg1	Cd					369:370	Cd	369:370	Cd	369:370	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	2	45	theme	toxic	342:346	arg1	cadmium					360:366	the toxic heavy metal cadmium	338:366	the toxic heavy metal cadmium (Cd)	338:371	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	6	46	theme	molecule	972:979	arg1	putrescine					981:990	the signaling molecule putrescine	958:990	the signaling molecule putrescine	958:990	Levels of the signaling molecule putrescine were significantly higher in the roots of both rice cultivars provided with NH4+ compared with NO3-.
30212760	7	47	theme	root	1225:1228	arg1	pectin					1240:1245	root cell wall pectin	1225:1245	root cell wall pectin	1225:1245	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	4	48	theme	negative	718:725	arg1	charges					727:733	the negative charges	714:733	the negative charges in the cell wall	714:750	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	1	49	theme	nutrient	178:185	arg1	uptake					187:192	nutrient uptake	178:192	nutrient uptake	178:192	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	9	50	theme	NH4+	1751:1754	arg1	fertilizers					1756:1766	NH4+ fertilizers	1751:1766	NH4+ fertilizers	1751:1766	Our findings suggest methods to decrease Cd accumulation in rice by applying NH4+ fertilizers.
30212760	9	51	theme	Cd	1715:1716	arg1	accumulation					1718:1729	Cd accumulation	1715:1729	Cd accumulation in rice	1715:1737	Our findings suggest methods to decrease Cd accumulation in rice by applying NH4+ fertilizers.
30212760	1	52	dep	nitrogen	147:154	arg1	NO3-					157:160	NO3-	157:160	NO3-	157:160	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	1	52	dep	nitrogen	147:154	arg1	NH4+					165:168	NH4+	165:168	NH4+	165:168	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	7	53	from	content	1195:1201	arg1	shoots					1206:1211	shoots	1206:1211	shoots	1206:1211	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	8	54	theme	Cd	1436:1437	arg1	toxicity					1439:1446	Cd toxicity	1436:1446	Cd toxicity	1436:1446	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	5	55	dep	absorption	832:841	arg1	the					828:830	the	828:830	the	828:830	In addition, NH4+ reduced the absorption and root-to-shoot translocation of Cd2+ by decreasing the expression of OsHMA2 and OsNramp5 in the root.
30212760	4	56	from	charges	727:733	arg1	wall					747:750	the cell wall	738:750	the cell wall	738:750	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	7	57	theme	rice	1150:1153	arg1	cultivars					1155:1163	both rice cultivars	1145:1163	both rice cultivars	1145:1163	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	8	58	theme	Cd-binding	1529:1538	arg1	capacity					1540:1547	the cell wall Cd-binding capacity	1515:1547	the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels	1515:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	4	59	theme	pectin	593:598	arg1	contents					618:625	the pectin and hemicellulose contents	589:625	contents	618:625	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	4	60	theme	pectin	645:650	arg1	PME					668:670	PME	668:670	PME	668:670	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	4	60	theme	pectin	645:650	arg1	methylesterase					652:665	pectin methylesterase	645:665	the pectin methylesterase (PME) activity in rice roots	641:694	NH4+ significantly reduced the pectin and hemicellulose contents and inhibited the pectin methylesterase (PME) activity in rice roots, thereby reducing the negative charges in the cell wall and decreasing the accumulation of Cd2+ in roots.
30212760	6	61	theme	rice	1039:1042	arg1	cultivars					1044:1052	both rice cultivars	1034:1052	both rice cultivars provided with NH4+ compared with NO3-	1034:1090	Levels of the signaling molecule putrescine were significantly higher in the roots of both rice cultivars provided with NH4+ compared with NO3-.
30212760	3	62	theme	Cd2+	466:469	arg1	levels					471:476	Cd2+ levels	466:476	Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+	466:559	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	7	63	theme	hemicellulose	1251:1263	arg1	contents					1265:1272	hemicellulose contents	1251:1272	hemicellulose contents	1251:1272	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	3	64	theme	NH4+	394:397	arg1	treatment					399:407	NH4+ treatment	394:407	NH4+ treatment	394:407	Compared with NO3-, NH4+ treatment significantly increased chlorophyll contents and reduced Cd2+ levels in rice cultivars Nipponbare (japonica) and Kasalath (indica) grown in 0.2 mM Cd2+.
30212760	8	65	theme	Cd	1609:1610	arg1	processes					1623:1631	processes	1623:1631	processes induced by increasing putrescine levels	1623:1671	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	8	65	theme	Cd	1609:1610	arg1	transport					1612:1620	Cd transport	1609:1620	Cd transport	1609:1620	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	8	66	theme	pectin	1571:1576	arg1	contents					1596:1603	pectin and hemicellulose contents	1571:1603	pectin and hemicellulose contents	1571:1603	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	0	67	dep	mitigates	9:17	arg1	Oryza					40:44	Oryza	40:44	Oryza	40:44	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	2	68	theme	Oryza	312:316	arg1	rice					306:309	rice	306:309	rice (Oryza sativa)	306:324	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	2	68	theme	Oryza	312:316	arg1	sativa					318:323	Oryza sativa	312:323	Oryza sativa	312:323	In the present study, we tested whether NO3- and NH4+ affect the ability of rice (Oryza sativa) to tolerate the toxic heavy metal cadmium (Cd).
30212760	7	69	theme	cell	1230:1233	arg1	pectin					1240:1245	root cell wall pectin	1225:1245	root cell wall pectin	1225:1245	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	1	70	theme	environmental	198:210	arg1	responses					219:227	environmental stress responses	198:227	environmental stress responses	198:227	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	7	71	theme	Cd2+	1128:1131	arg1	contents					1133:1140	Cd2+ contents	1128:1140	Cd2+ contents	1128:1140	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	0	72	from	toxicity	22:29	arg1	rice					34:37	rice	34:37	rice	34:37	Ammonium mitigates Cd toxicity in rice (Oryza sativa) via putrescine-dependent alterations of cell wall composition.
30212760	8	73	theme	Cd	1499:1500	arg1	effects					1488:1494	the noxious effects	1476:1494	the noxious effects of Cd	1476:1500	Taken together, these results indicate that NH4+ treatment alleviated Cd toxicity, enabling rice to withstand the noxious effects of Cd by modifying the cell wall Cd-binding capacity due to alterations of pectin and hemicellulose contents and Cd transport, processes induced by increasing putrescine levels.
30212760	1	74	theme	different	128:136	arg1	forms					138:142	different forms	128:142	different forms of nitrogen (NO3- or NH4+)	128:169	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30212760	7	75	from	expression	1319:1328	arg1	root					1360:1363	the root	1356:1363	the root	1356:1363	The addition of putrescine reduced Cd2+ contents in both rice cultivars and increased the chlorophyll content in shoots by reducing root cell wall pectin and hemicellulose contents, inhibiting PME activity and suppressing the expression of OsHMA2 and OsNramp5 in the root.
30212760	1	76	theme	stress	212:217	arg1	responses					219:227	environmental stress responses	198:227	environmental stress responses	198:227	In plants, different forms of nitrogen (NO3- or NH4+) affect nutrient uptake and environmental stress responses.
30807652	0	0	theme	aminoalcohols	117:129	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	3	1	theme	selector	853:860	arg1	nature					832:837	the nature	828:837	the nature of the chiral selector	828:860	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	1	theme	selector	853:860	arg1	method					812:817	the chromatographic method	792:817	the chromatographic method applied	792:825	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	1	theme	selector	853:860	arg1	details					881:887	the structural details	866:887	the structural details of the analytes	866:903	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	4	2	theme	retention	1029:1037	arg1	times					1039:1043	decreased retention times	1019:1043	decreased retention times	1019:1043	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	1	3	theme	limonene-based	217:230	arg1	1,3-aminoalcohols					241:257	limonene-based bicyclic 1,3-aminoalcohols	217:257	limonene-based bicyclic 1,3-aminoalcohols	217:257	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	3	4	theme	structural	870:879	arg1	details					881:887	the structural details	866:887	the structural details of the analytes	866:903	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	0	5	theme	bicyclic	108:115	arg1	aminoalcohols					117:129	limonene-based bicyclic aminoalcohols	93:129	limonene-based bicyclic aminoalcohols	93:129	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	4	6	theme	decreased	1019:1027	arg1	times					1039:1043	decreased retention times	1019:1043	decreased retention times	1019:1043	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	1	7	theme	bicyclic	232:239	arg1	1,3-aminoalcohols					241:257	limonene-based bicyclic 1,3-aminoalcohols	217:257	limonene-based bicyclic 1,3-aminoalcohols	217:257	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	1	8	theme	supercritical	365:377	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	9	theme	aminodiols	135:144	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	1	10	theme	fluid	379:383	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	11	from	enantioseparations	71:88	arg1	phases					188:193	polysaccharide-based chiral stationary phases	149:193	polysaccharide-based chiral stationary phases	149:193	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	2	12	from	effects	469:475	arg1	selectivity					613:623	selectivity	613:623	selectivity	613:623	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	12	from	effects	469:475	arg1	retention					599:607	retention	599:607	retention	599:607	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	3	13	attach	derived	706:712	arg1	studies					754:760	selectivity-temperature-dependence studies	719:760	selectivity-temperature-dependence studies	719:760	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	13	attach	derived	706:712	arg2	parameters					695:704	Thermodynamic parameters	681:704	Thermodynamic parameters derived from selectivity-temperature-dependence studies	681:760	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	1	14	theme	chromatographic	385:399	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	15	theme	polysaccharide-based	149:168	arg1	phases					188:193	polysaccharide-based chiral stationary phases	149:193	polysaccharide-based chiral stationary phases	149:193	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	2	16	theme	SFC	666:668	arg1	technique					670:678	SFC technique	666:678	SFC technique	666:678	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	4	17	from	Enantiorecognition	906:923	arg1	cases					933:937	most cases	928:937	most cases	928:937	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	2	18	theme	mobile	503:508	arg1	phase					510:514	the mobile phase	499:514	the mobile phase	499:514	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	1	19	theme	1,3-aminoalcohols	241:257	arg1	Enantioseparation					196:212	Enantioseparation	196:212	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols	196:289	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	2	20	theme	analytes	566:573	arg1	temperature					528:538	the column temperature	517:538	the column temperature	517:538	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	20	theme	analytes	566:573	arg1	composition					484:494	the composition	480:494	the composition of the mobile phase	480:514	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	20	theme	analytes	566:573	arg1	structures					548:557	the structures	544:557	the structures of the analytes and chiral selectors	544:594	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	21	theme	selectors	586:594	arg1	temperature					528:538	the column temperature	517:538	the column temperature	517:538	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	21	theme	selectors	586:594	arg1	composition					484:494	the composition	480:494	the composition of the mobile phase	480:514	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	21	theme	selectors	586:594	arg1	structures					548:557	the structures	544:557	the structures of the analytes and chiral selectors	544:594	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	4	22	theme	most	928:931	arg1	cases					933:937	most cases	928:937	most cases	928:937	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	2	23	theme	phase	510:514	arg1	temperature					528:538	the column temperature	517:538	the column temperature	517:538	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	23	theme	phase	510:514	arg1	composition					484:494	the composition	480:494	the composition of the mobile phase	480:514	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	2	23	theme	phase	510:514	arg1	structures					548:557	the structures	544:557	the structures of the analytes and chiral selectors	544:594	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	0	24	theme	high-performance	7:22	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	6	25	theme	stereoisomers	1256:1268	arg1	separation					1238:1247	The separation	1234:1247	The separation of the stereoisomers	1234:1268	The separation of the stereoisomers was optimized in both chromatographic modalities.
30807652	1	26	theme	1,3,5-	263:268	arg1	Enantioseparation					196:212	Enantioseparation	196:212	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols	196:289	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	27	theme	Chiral	0:5	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	3	28	theme	analytes	896:903	arg1	nature					832:837	the nature	828:837	the nature of the chiral selector	828:860	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	28	theme	analytes	896:903	arg1	method					812:817	the chromatographic method	792:817	the chromatographic method applied	792:825	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	28	theme	analytes	896:903	arg1	details					881:887	the structural details	866:887	the structural details of the analytes	866:903	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	2	29	theme	structures	548:557	arg1	effects					469:475	The effects	465:475	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity	465:623	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	1	30	theme	SFC	402:404	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	31	theme	stationary	177:186	arg1	phases					188:193	polysaccharide-based chiral stationary phases	149:193	polysaccharide-based chiral stationary phases	149:193	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	4	32	theme	temperature	979:989	arg1	behavior					991:998	an unusual temperature behavior	968:998	an unusual temperature behavior	968:998	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	2	33	theme	normal-phase	646:657	arg1	LC					659:660	normal-phase LC	646:660	normal-phase LC	646:660	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	0	34	theme	liquid	24:29	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	0	35	theme	chiral	170:175	arg1	phases					188:193	polysaccharide-based chiral stationary phases	149:193	polysaccharide-based chiral stationary phases	149:193	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	3	36	theme	chromatographic	796:810	arg1	method					812:817	the chromatographic method	792:817	the chromatographic method applied	792:825	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	3	37	theme	Thermodynamic	681:693	arg1	parameters					695:704	Thermodynamic parameters	681:704	Thermodynamic parameters derived from selectivity-temperature-dependence studies	681:760	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	2	38	theme	temperature	528:538	arg1	effects					469:475	The effects	465:475	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity	465:623	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	0	39	theme	fluid	49:53	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	4	40	theme	unusual	971:977	arg1	behavior					991:998	an unusual temperature behavior	968:998	an unusual temperature behavior	968:998	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	2	41	theme	column	521:526	arg1	temperature					528:538	the column temperature	517:538	the column temperature	517:538	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	0	42	theme	supercritical	35:47	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	2	43	theme	chiral	579:584	arg1	selectors					586:594	chiral selectors	579:594	chiral selectors	579:594	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	1	44	theme	polysaccharide-based	418:437	arg1	phases					457:462	polysaccharide-based chiral stationary phases	418:462	polysaccharide-based chiral stationary phases	418:462	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	4	45	dep	separations	1152:1162	arg1	i.e.					1122:1125	i.e.	1122:1125	i.e.	1122:1125	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	0	46	theme	chromatographic	55:69	arg1	enantioseparations					71:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations	0:88	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.	0:194	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	1	47	theme	chiral	439:444	arg1	phases					457:462	polysaccharide-based chiral stationary phases	418:462	polysaccharide-based chiral stationary phases	418:462	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	3	48	theme	selectivity-temperature-dependence	719:752	arg1	studies					754:760	selectivity-temperature-dependence studies	719:760	selectivity-temperature-dependence studies	719:760	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	1	49	theme	stationary	446:455	arg1	phases					457:462	polysaccharide-based chiral stationary phases	418:462	polysaccharide-based chiral stationary phases	418:462	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	4	50	theme	separation	1074:1083	arg1	factors					1085:1091	improved separation factors	1065:1091	improved separation factors	1065:1091	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	1	51	theme	normal-phase	308:319	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	4	52	theme	improved	1065:1072	arg1	factors					1085:1091	improved separation factors	1065:1091	improved separation factors	1065:1091	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	6	53	theme	chromatographic	1292:1306	arg1	modalities					1308:1317	both chromatographic modalities	1287:1317	both chromatographic modalities	1287:1317	The separation of the stereoisomers was optimized in both chromatographic modalities.
30807652	1	54	theme	high-performance	321:336	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	0	55	theme	limonene-based	93:106	arg1	aminoalcohols					117:129	limonene-based bicyclic aminoalcohols	93:129	limonene-based bicyclic aminoalcohols	93:129	Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.
30807652	1	56	theme	liquid	338:343	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
30807652	4	57	theme	driven	1145:1150	arg1	separations					1152:1162	some entropically driven separations	1127:1162	some entropically driven separations were also observed	1127:1181	Enantiorecognition in most cases was enthalpically driven but an unusual temperature behavior was also observed: decreased retention times were accompanied by improved separation factors with increasing temperature, i.e. some entropically driven separations were also observed.
30807652	3	58	theme	chiral	846:851	arg1	selector					853:860	the chiral selector	842:860	the chiral selector	842:860	Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes.
30807652	5	59	theme	elution	1188:1194	arg1	sequence					1196:1203	The elution sequence	1184:1203	The elution sequence	1184:1203	The elution sequence was determined in all cases.
30807652	2	60	theme	composition	484:494	arg1	effects					469:475	The effects	465:475	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity	465:623	The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique.
30807652	1	61	theme	chromatographic	345:359	arg1	methods					407:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods	308:413	Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases.
31470054	5	0	theme	thermal	874:880	arg1	best					927:930	best	927:930	best	927:930	Among the films tested, the thermal stability of the 0.15PUL:0.15BG film was the best.
31470054	5	0	theme	thermal	874:880	arg1	stability					882:890	the thermal stability	870:890	the thermal stability of the 0.15PUL:0.15BG film	870:917	Among the films tested, the thermal stability of the 0.15PUL:0.15BG film was the best.
31470054	0	1	theme	films	84:88	arg1	properties					13:22	properties	13:22	properties	13:22	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	0	1	theme	films	84:88	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	0	1	theme	films	84:88	arg1	characterization					40:55	structural characterization	29:55	structural characterization of β-glucan/pullulan blend films	29:88	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	4	2	theme	bonding	679:685	arg1	interactions					687:698	hydrogen bonding interactions	670:698	hydrogen bonding interactions between PUL and BG molecules	670:727	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	1	3	theme	BG	257:258	arg1	0-0.3 g					246:252	0-0.3 g	246:252	0-0.3 g of BG	246:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	7	4	theme	PUL-based	1077:1085	arg1	films					1094:1098	PUL-based edible films	1077:1098	PUL-based edible films	1077:1098	This research offers an alternative method to improve PUL-based edible films.
31470054	4	5	theme	continuous	827:836	arg1	matrix					838:843	the PUL continuous matrix	819:843	the PUL continuous matrix	819:843	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	6	6	theme	0.15:0.15	958:966	arg1	ratio					949:953	A PUL:BG mixing ratio	933:953	A PUL:BG mixing ratio of 0.15:0.15	933:966	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	7	7	theme	edible	1087:1092	arg1	films					1094:1098	PUL-based edible films	1077:1098	PUL-based edible films	1077:1098	This research offers an alternative method to improve PUL-based edible films.
31470054	2	8	theme	BG	287:288	arg1	addition					290:297	BG addition	287:297	BG addition	287:297	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	3	9	theme	oxygen	478:483	arg1	property					493:500	the oxygen barrier property	474:500	the oxygen barrier property of the 0.15PUL:0.15BG film	474:527	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	10	theme	film	465:468	arg1	transparency					432:443	The transparency	428:443	The transparency of the 0.2PUL:0.1BG film	428:468	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	10	theme	film	465:468	arg1	property					493:500	the oxygen barrier property	474:500	the oxygen barrier property of the 0.15PUL:0.15BG film	474:527	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	11	theme	film	524:527	arg1	transparency					432:443	The transparency	428:443	The transparency of the 0.2PUL:0.1BG film	428:468	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	11	theme	film	524:527	arg1	property					493:500	the oxygen barrier property	474:500	the oxygen barrier property of the 0.15PUL:0.15BG film	474:527	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	2	12	theme	dissolution	387:397	arg1	time					399:402	break (p < 0.05), tensile strength, and water dissolution time	341:402	break (p < 0.05), tensile strength, and water dissolution time of the resulting films	341:425	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	1	13	theme	/pullulan	180:188	arg1	PUL					191:193	PUL	191:193	PUL	191:193	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	1	13	theme	/pullulan	180:188	arg1	films					213:217	β-glucan (BG)/pullulan (PUL) composite edible films	167:217	β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	167:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	7	14	theme	alternative	1047:1057	arg1	method					1059:1064	an alternative method	1044:1064	an alternative method to improve PUL-based edible films	1044:1098	This research offers an alternative method to improve PUL-based edible films.
31470054	6	15	theme	mixing	942:947	arg1	ratio					949:953	A PUL:BG mixing ratio	933:953	A PUL:BG mixing ratio of 0.15:0.15	933:966	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	4	16	theme	microstructural	734:748	arg1	observations					750:761	microstructural observations	734:761	microstructural observations	734:761	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	2	17	theme	resulting	411:419	arg1	films					421:425	the resulting films	407:425	the resulting films	407:425	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	2	18	theme	films	421:425	arg1	time					399:402	break (p < 0.05), tensile strength, and water dissolution time	341:402	break (p < 0.05), tensile strength, and water dissolution time of the resulting films	341:425	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	6	19	theme	BG	939:940	arg1	ratio					949:953	A PUL:BG mixing ratio	933:953	A PUL:BG mixing ratio of 0.15:0.15	933:966	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	4	20	theme	aggregated	775:784	arg1	BG					786:787	aggregated BG	775:787	aggregated BG	775:787	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	1	21	theme	composite	196:204	arg1	PUL					191:193	PUL	191:193	PUL	191:193	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	1	21	theme	composite	196:204	arg1	films					213:217	β-glucan (BG)/pullulan (PUL) composite edible films	167:217	β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	167:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	0	22	theme	structural	29:38	arg1	characterization					40:55	structural characterization	29:55	structural characterization of β-glucan/pullulan blend films	29:88	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	5	23	theme	film	914:917	arg1	best					927:930	best	927:930	best	927:930	Among the films tested, the thermal stability of the 0.15PUL:0.15BG film was the best.
31470054	5	23	theme	film	914:917	arg1	stability					882:890	the thermal stability	870:890	the thermal stability of the 0.15PUL:0.15BG film	870:917	Among the films tested, the thermal stability of the 0.15PUL:0.15BG film was the best.
31470054	6	24	theme	film	1006:1009	arg1	properties					1011:1020	the best film properties	997:1020	the best film properties	997:1020	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	1	25	theme	edible	206:211	arg1	PUL					191:193	PUL	191:193	PUL	191:193	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	1	25	theme	edible	206:211	arg1	films					213:217	β-glucan (BG)/pullulan (PUL) composite edible films	167:217	β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	167:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	4	26	theme	hydrogen	670:677	arg1	interactions					687:698	hydrogen bonding interactions	670:698	hydrogen bonding interactions between PUL and BG molecules	670:727	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	6	27	theme	PUL	935:937	arg1	ratio					949:953	A PUL:BG mixing ratio	933:953	A PUL:BG mixing ratio of 0.15:0.15	933:966	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	3	28	theme	other	621:625	arg1	films					637:641	other composite films	621:641	other composite films (p < 0.05)	621:652	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	28	theme	other	621:625	arg1	p < 0.05					644:651	p < 0.05	644:651	p < 0.05	644:651	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	1	29	theme	films	213:217	arg1	properties					153:162	the physico-mechanical and structural properties	115:162	the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	115:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	3	30	dep	films	583:587	arg1	0PUL:0.3BG					605:614	0PUL:0.3BG	605:614	0PUL:0.3BG	605:614	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	30	dep	films	583:587	arg1	0.3PUL:0BG					590:599	0.3PUL:0BG	590:599	0.3PUL:0BG	590:599	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	30	dep	films	583:587	arg1	films					583:587	the plain films	573:587	the plain films (0.3PUL:0BG and 0PUL:0.3BG)	573:615	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	31	theme	composite	627:635	arg1	films					637:641	other composite films	621:641	other composite films (p < 0.05)	621:652	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	31	theme	composite	627:635	arg1	p < 0.05					644:651	p < 0.05	644:651	p < 0.05	644:651	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	32	theme	plain	577:581	arg1	0PUL:0.3BG					605:614	0PUL:0.3BG	605:614	0PUL:0.3BG	605:614	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	32	theme	plain	577:581	arg1	0.3PUL:0BG					590:599	0.3PUL:0BG	590:599	0.3PUL:0BG	590:599	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	3	32	theme	plain	577:581	arg1	films					583:587	the plain films	573:587	the plain films (0.3PUL:0BG and 0PUL:0.3BG)	573:615	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	1	33	theme	physico-mechanical	119:136	arg1	properties					153:162	the physico-mechanical and structural properties	115:162	the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	115:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	4	34	theme	PUL	823:825	arg1	matrix					838:843	the PUL continuous matrix	819:843	the PUL continuous matrix	819:843	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	3	35	theme	barrier	485:491	arg1	property					493:500	the oxygen barrier property	474:500	the oxygen barrier property of the 0.15PUL:0.15BG film	474:527	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	1	36	theme	structural	142:151	arg1	properties					153:162	the physico-mechanical and structural properties	115:162	the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG	115:258	This study investigates the physico-mechanical and structural properties of β-glucan (BG)/pullulan (PUL) composite edible films successfully prepared with 0-0.3 g of BG.
31470054	5	37	theme	0.15PUL:0.15BG	899:912	arg1	film					914:917	the 0.15PUL:0.15BG film	895:917	the 0.15PUL:0.15BG film	895:917	Among the films tested, the thermal stability of the 0.15PUL:0.15BG film was the best.
31470054	4	38	theme	BG	716:717	arg1	molecules					719:727	BG molecules	716:727	BG molecules	716:727	FTIR indicated hydrogen bonding interactions between PUL and BG molecules, and microstructural observations showed that aggregated BG is homogeneously dispersed in the PUL continuous matrix.
31470054	3	39	theme	0.15PUL:0.15BG	509:522	arg1	film					524:527	the 0.15PUL:0.15BG film	505:527	the 0.15PUL:0.15BG film	505:527	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	2	40	theme	break	341:345	arg1	time					399:402	break (p < 0.05), tensile strength, and water dissolution time	341:402	break (p < 0.05), tensile strength, and water dissolution time of the resulting films	341:425	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	0	41	theme	blend	78:82	arg1	films					84:88	β-glucan/pullulan blend films	60:88	β-glucan/pullulan blend films	60:88	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	6	42	theme	best	1001:1004	arg1	properties					1011:1020	the best film properties	997:1020	the best film properties	997:1020	A PUL:BG mixing ratio of 0.15:0.15 is thus suggested to provide the best film properties.
31470054	3	43	theme	0.2PUL:0.1BG	452:463	arg1	film					465:468	the 0.2PUL:0.1BG film	448:468	the 0.2PUL:0.1BG film	448:468	The transparency of the 0.2PUL:0.1BG film and the oxygen barrier property of the 0.15PUL:0.15BG film decreased remarkably compared with those of the plain films (0.3PUL:0BG and 0PUL:0.3BG) and other composite films (p < 0.05).
31470054	2	44	theme	strength	367:374	arg1	time					399:402	break (p < 0.05), tensile strength, and water dissolution time	341:402	break (p < 0.05), tensile strength, and water dissolution time of the resulting films	341:425	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	2	45	theme	water	381:385	arg1	dissolution					387:397	water dissolution	381:397	water dissolution	381:397	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31470054	0	46	theme	β-glucan/pullulan	60:76	arg1	films					84:88	β-glucan/pullulan blend films	60:88	β-glucan/pullulan blend films	60:88	Preparation, properties, and structural characterization of β-glucan/pullulan blend films.
31470054	2	47	theme	tensile	359:365	arg1	strength					367:374	tensile strength	359:374	tensile strength	359:374	Results demonstrated that BG addition significantly increases the elongation at break (p < 0.05), tensile strength, and water dissolution time of the resulting films.
31102677	11	0	theme	tissue	1497:1502	arg1	engineering					1504:1514	tissue engineering	1497:1514	tissue engineering scaffold	1497:1523	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	3	1	from	length	452:457	arg1	scale					473:477	sub-micron scale	462:477	sub-micron scale	462:477	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	3	1	from	length	452:457	arg1	scale					442:446	nano scale	437:446	nano scale	437:446	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	5	2	theme	network	711:717	arg1	structure					719:727	fine three-dimensional network structure	688:727	fine three-dimensional network structure	688:727	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	1	3	theme	good	178:181	arg1	biocompatibility					183:198	good biocompatibility	178:198	good biocompatibility	178:198	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	7	4	theme	CMCS	916:919	arg1	hydrogel					931:938	CMCS composite hydrogel	916:938	CMCS composite hydrogel	916:938	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	7	4	theme	CMCS	916:919	arg1	result					908:913	a result	906:913	a result	906:913	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	4	5	theme	MFC	557:559	arg1	surface					573:579	the MFC nanofibrils surface	553:579	the MFC nanofibrils surface	553:579	The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups.
31102677	10	6	theme	%	1351:1351	arg1	%					1330:1330	5 to 15 wt%	1320:1330	5 to 15 wt%	1320:1330	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	6	theme	%	1351:1351	arg1	increase					1336:1343	an increase	1333:1343	an increase of 330% and 338%, respectively	1333:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	5	7	theme	fine	688:691	arg1	structure					719:727	fine three-dimensional network structure	688:727	fine three-dimensional network structure	688:727	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	7	8	theme	pure	980:983	arg1	CMCS					985:988	pure CMCS	980:988	pure CMCS	980:988	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	0	9	theme	gelation	122:129	arg1	formability					131:141	quick gelation formability	116:141	quick gelation formability	116:141	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	10	10	theme	%	1360:1360	arg1	%					1330:1330	5 to 15 wt%	1320:1330	5 to 15 wt%	1320:1330	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	10	theme	%	1360:1360	arg1	increase					1336:1343	an increase	1333:1343	an increase of 330% and 338%, respectively	1333:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	11	theme	hydrogel	1247:1254	arg1	content					1296:1302	the DAMFC content	1286:1302	the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively	1286:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	11	theme	hydrogel	1247:1254	arg1	strength					1206:1213	The compression strength	1190:1213	The compression strength	1190:1213	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	11	theme	hydrogel	1247:1254	arg1	work					1223:1226	the work	1219:1226	the work of fracture	1219:1238	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	0	12	theme	quick	116:120	arg1	formability					131:141	quick gelation formability	116:141	quick gelation formability	116:141	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	3	13	from	diameter	425:432	arg1	scale					473:477	sub-micron scale	462:477	sub-micron scale	462:477	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	3	13	from	diameter	425:432	arg1	scale					442:446	nano scale	437:446	nano scale	437:446	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	2	14	theme	network	333:339	arg1	structure					341:349	giant network structure	327:349	giant network structure	327:349	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	6	15	theme	Schiff-base	762:772	arg1	reaction					774:781	the Schiff-base reaction	758:781	the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains	758:858	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	4	16	theme	hydroxyl	534:541	arg1	groups					543:548	The hydroxyl groups	530:548	The hydroxyl groups on the MFC nanofibrils surface	530:579	The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups.
31102677	8	17	theme	reinforcing	1050:1060	arg1	agent					1062:1066	a reinforcing agent	1048:1066	a reinforcing agent as well as a macroscopical crosslinking agent	1048:1112	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	8	17	theme	reinforcing	1050:1060	arg1	DAMFC					1033:1037	DAMFC	1033:1037	DAMFC	1033:1037	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	5	18	theme	fibril	666:671	arg1	morphology					673:682	the fibril morphology	662:682	the fibril morphology	662:682	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	2	19	theme	giant	327:331	arg1	structure					341:349	giant network structure	327:349	giant network structure	327:349	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	10	20	theme	DAMFC	1290:1294	arg1	content					1296:1302	the DAMFC content	1286:1302	the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively	1286:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	20	theme	DAMFC	1290:1294	arg1	strength					1206:1213	The compression strength	1190:1213	The compression strength	1190:1213	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	20	theme	DAMFC	1290:1294	arg1	work					1223:1226	the work	1219:1226	the work of fracture	1219:1238	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	1	21	contain	has	174:176	arg2	biocompatibility					183:198	good biocompatibility	178:198	good biocompatibility	178:198	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	1	21	contain	has	174:176	arg1	CMCS					168:171	CMCS	168:171	CMCS	168:171	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	1	21	contain	has	174:176	arg1	chitosan					158:165	Carboxymethyl chitosan	144:165	Carboxymethyl chitosan (CMCS)	144:172	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	1	21	contain	has	174:176	arg2	biodegradability					201:216	biodegradability	201:216	biodegradability	201:216	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	1	21	contain	has	174:176	arg2	solubility					228:237	water solubility	222:237	water solubility	222:237	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	7	22	theme	short	959:963	arg1	time					965:968	short time	959:968	short time	959:968	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	9	23	dep	structure	1119:1127	arg1	The					1115:1117	The	1115:1117	The	1115:1117	The structure and properties of the composite hydrogels were investigated.
31102677	4	24	theme	aldehyde	607:614	arg1	groups					616:621	aldehyde groups	607:621	aldehyde groups	607:621	The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups.
31102677	3	25	theme	nano	437:440	arg1	scale					442:446	nano scale	437:446	nano scale	437:446	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	4	26	from	groups	543:548	arg1	surface					573:579	the MFC nanofibrils surface	553:579	the MFC nanofibrils surface	553:579	The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups.
31102677	3	27	theme	microfibrillated	387:402	arg1	MFC					415:417	MFC	415:417	MFC	415:417	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	3	27	theme	microfibrillated	387:402	arg1	cellulose					404:412	The microfibrillated cellulose	383:412	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale	383:477	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	0	28	theme	Modified	0:7	arg1	nano					9:12	Modified nano	0:12	Modified nano	0:12	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	11	29	theme	engineering	1504:1514	arg1	scaffold					1516:1523	tissue engineering scaffold	1497:1523	tissue engineering scaffold	1497:1523	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	5	30	theme	three-dimensional	693:709	arg1	structure					719:727	fine three-dimensional network structure	688:727	fine three-dimensional network structure	688:727	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	6	31	theme	amino	832:836	arg1	groups					838:843	amino groups	832:843	amino groups	832:843	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	2	32	theme	gelation	361:368	arg1	formability					370:380	quick gelation formability	355:380	quick gelation formability	355:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	11	33	theme	hydrogel	1395:1402	arg1	images					1381:1386	SEM images	1377:1386	SEM images of the hydrogel	1377:1402	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	1	34	theme	water	222:226	arg1	solubility					228:237	water solubility	222:237	water solubility	222:237	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	0	35	theme	cellulose/carboxymethyl	31:53	arg1	hydrogel					74:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	6	36	theme	CMCS	848:851	arg1	chains					853:858	CMCS chains	848:858	CMCS chains	848:858	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	2	37	theme	quick	355:359	arg1	formability					370:380	quick gelation formability	355:380	quick gelation formability	355:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	10	38	dep	15 wt	1325:1329	arg1	to					1322:1323	to	1322:1323	to	1322:1323	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	11	39	theme	three-dimensional	1418:1434	arg1	structure					1443:1451	a fine three-dimensional porous structure	1411:1451	a fine three-dimensional porous structure	1411:1451	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	0	40	theme	composite	64:72	arg1	hydrogel					74:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	8	41	theme	macroscopical	1081:1093	arg1	agent					1108:1112	a macroscopical crosslinking agent	1079:1112	a reinforcing agent as well as a macroscopical crosslinking agent	1048:1112	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	8	41	theme	macroscopical	1081:1093	arg1	DAMFC					1033:1037	DAMFC	1033:1037	DAMFC	1033:1037	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	11	42	theme	fine	1413:1416	arg1	structure					1443:1451	a fine three-dimensional porous structure	1411:1451	a fine three-dimensional porous structure	1411:1451	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	3	43	theme	high-pressure	500:512	arg1	homogenization					514:527	high-pressure homogenization	500:527	high-pressure homogenization	500:527	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	0	44	theme	chitosan	55:62	arg1	hydrogel					74:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	cellulose/carboxymethyl chitosan composite hydrogel	31:81	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	3	45	theme	sub-micron	462:471	arg1	scale					473:477	sub-micron scale	462:477	sub-micron scale	462:477	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	2	46	dep	nanocellulose	265:277	arg1	reinforced					287:296	reinforced	287:296	reinforced CMCS composite hydrogel with giant network structure and quick gelation formability	287:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	11	47	theme	SEM	1377:1379	arg1	images					1381:1386	SEM images	1377:1386	SEM images of the hydrogel	1377:1402	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	6	48	theme	DAMFC	814:818	arg1	surface					820:826	DAMFC surface	814:826	DAMFC surface	814:826	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	1	49	theme	Carboxymethyl	144:156	arg1	CMCS					168:171	CMCS	168:171	CMCS	168:171	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	1	49	theme	Carboxymethyl	144:156	arg1	chitosan					158:165	Carboxymethyl chitosan	144:165	Carboxymethyl chitosan (CMCS)	144:172	Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility.
31102677	2	50	with	hydrogel	313:320	arg1	structure					341:349	giant network structure	327:349	giant network structure	327:349	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	2	50	with	hydrogel	313:320	arg1	formability					370:380	quick gelation formability	355:380	quick gelation formability	355:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	8	51	theme	crosslinking	1095:1106	arg1	agent					1108:1112	a macroscopical crosslinking agent	1079:1112	a reinforcing agent as well as a macroscopical crosslinking agent	1048:1112	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	8	51	theme	crosslinking	1095:1106	arg1	DAMFC					1033:1037	DAMFC	1033:1037	DAMFC	1033:1037	In the composite, DAMFC acted as a reinforcing agent as well as a macroscopical crosslinking agent.
31102677	6	52	from	groups	804:809	arg1	surface					820:826	DAMFC surface	814:826	DAMFC surface	814:826	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	6	53	from	groups	838:843	arg1	surface					820:826	DAMFC surface	814:826	DAMFC surface	814:826	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	5	54	theme	dialdehyde	628:637	arg1	DAMFC					644:648	DAMFC	644:648	DAMFC	644:648	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	5	54	theme	dialdehyde	628:637	arg1	MFC					639:641	The dialdehyde MFC	624:641	The dialdehyde MFC (DAMFC)	624:649	The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS.
31102677	10	55	theme	compression	1194:1204	arg1	content					1296:1302	the DAMFC content	1286:1302	the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively	1286:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	55	theme	compression	1194:1204	arg1	strength					1206:1213	The compression strength	1190:1213	The compression strength	1190:1213	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	55	theme	compression	1194:1204	arg1	work					1223:1226	the work	1219:1226	the work of fracture	1219:1238	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	56	theme	fracture	1231:1238	arg1	content					1296:1302	the DAMFC content	1286:1302	the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively	1286:1374	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	56	theme	fracture	1231:1238	arg1	strength					1206:1213	The compression strength	1190:1213	The compression strength	1190:1213	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	10	56	theme	fracture	1231:1238	arg1	work					1223:1226	the work	1219:1226	the work of fracture	1219:1238	The compression strength and the work of fracture of the hydrogel was increased dramatically as the DAMFC content increasing from 5 to 15 wt%, an increase of 330% and 338%, respectively.
31102677	2	57	theme	composite	303:311	arg1	hydrogel					313:320	CMCS composite hydrogel	298:320	CMCS composite hydrogel with giant network structure and quick gelation formability	298:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	6	58	theme	aldehyde	795:802	arg1	groups					804:809	the aldehyde groups	791:809	the aldehyde groups on DAMFC surface	791:826	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	11	59	theme	porous	1436:1441	arg1	structure					1443:1451	a fine three-dimensional porous structure	1411:1451	a fine three-dimensional porous structure	1411:1451	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31102677	7	60	theme	composite	921:929	arg1	hydrogel					931:938	CMCS composite hydrogel	916:938	CMCS composite hydrogel	916:938	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	7	60	theme	composite	921:929	arg1	result					908:913	a result	906:913	a result	906:913	As a result, CMCS composite hydrogel could be formed in short time, although pure CMCS could not form hydrogel.
31102677	0	61	theme	network	94:100	arg1	structure					102:110	giant network structure	88:110	giant network structure	88:110	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	6	62	theme	giant	863:867	arg1	network					869:875	a giant network	861:875	a giant network	861:875	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	2	63	theme	CMCS	298:301	arg1	hydrogel					313:320	CMCS composite hydrogel	298:320	CMCS composite hydrogel with giant network structure and quick gelation formability	298:380	This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability.
31102677	9	64	theme	composite	1151:1159	arg1	hydrogels					1161:1169	the composite hydrogels	1147:1169	the composite hydrogels	1147:1169	The structure and properties of the composite hydrogels were investigated.
31102677	0	65	theme	giant	88:92	arg1	structure					102:110	giant network structure	88:110	giant network structure	88:110	Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
31102677	4	66	theme	nanofibrils	561:571	arg1	surface					573:579	the MFC nanofibrils surface	553:579	the MFC nanofibrils surface	553:579	The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups.
31102677	3	67	with	cellulose	404:412	arg1	length					452:457	length	452:457	length in sub-micron scale	452:477	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	3	67	with	cellulose	404:412	arg1	diameter					425:432	diameter	425:432	diameter in nano scale	425:446	The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization.
31102677	9	68	theme	hydrogels	1161:1169	arg1	properties					1133:1142	properties	1133:1142	properties	1133:1142	The structure and properties of the composite hydrogels were investigated.
31102677	9	68	theme	hydrogels	1161:1169	arg1	structure					1119:1127	structure	1119:1127	structure	1119:1127	The structure and properties of the composite hydrogels were investigated.
31102677	6	69	from	reaction	774:781	arg1	chains					853:858	CMCS chains	848:858	CMCS chains	848:858	Due to the Schiff-base reaction between the aldehyde groups on DAMFC surface and amino groups on CMCS chains, a giant network was formed in DAMFC/CMCS.
31102677	11	70	theme	promising	1474:1482	arg1	platform					1484:1491	a promising platform	1472:1491	a promising platform for tissue engineering scaffold	1472:1523	SEM images of the hydrogel showed a fine three-dimensional porous structure, which may provide a promising platform for tissue engineering scaffold.
31581350	6	0	theme	predicted	1249:1257	arg1	values					1267:1272	the predicted kinetic values	1245:1272	the predicted kinetic values	1245:1272	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	3	1	theme	ZnSO4	584:588	arg1	O					595:595	ZnSO4 .7H2 O	584:595	ZnSO4 .7H2 O	584:595	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	6	2	theme	Baranyi	1164:1170	arg1	models					1181:1186	Baranyi and Cone models	1164:1186	Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively	1164:1286	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	1	3	from	extract	164:170	arg1	inulinase					143:151	inulinase	143:151	inulinase from carob extract	143:170	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	5	4	theme	inulinase	755:763	arg1	1507.03 U/ml					938:949	1507.03 U/ml	938:949	1507.03 U/ml	938:949	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	5	4	theme	inulinase	755:763	arg1	activity					765:772	the maximum inulinase activity	743:772	the maximum inulinase activity	743:772	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	0	5	theme	Aspergillus	75:85	arg1	niger					87:91	Aspergillus niger	75:91	Aspergillus niger	75:91	Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
31581350	7	6	theme	carob	1338:1342	arg1	extract					1344:1350	carob extract	1338:1350	carob extract	1338:1350	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	7	6	theme	carob	1338:1342	arg1	source					1404:1409	a carbon source	1395:1409	a carbon source	1395:1409	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	4	7	theme	medium	641:646	arg1	composition					648:658	optimum medium composition	633:658	optimum medium composition	633:658	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	8	8	theme	flask	1552:1556	arg1	fermentation					1558:1569	shake flask fermentation	1546:1569	shake flask fermentation	1546:1569	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	1	9	theme	inulinase	228:236	arg1	fermentation					238:249	the inulinase fermentation	224:249	the inulinase fermentation in the optimum carob extract-based medium	224:291	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	0	10	from	extract	58:64	arg1	production					10:19	production	10:19	production	10:19	Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
31581350	0	10	from	extract	58:64	arg1	modeling					38:45	mathematical modeling	25:45	mathematical modeling	25:45	Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
31581350	2	11	used	used	326:329	arg2	source					363:368	a novel and renewable carbon source	334:368	a novel and renewable carbon source in the production of A. niger inulinase	334:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	11	used	used	326:329	arg2	extract					314:320	carob extract	308:320	carob extract	308:320	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	4	12	theme	optimum	633:639	arg1	composition					648:658	optimum medium composition	633:658	optimum medium composition	633:658	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	8	13	theme	carob	1576:1580	arg1	medium					1590:1595	carob extract medium	1576:1595	carob extract medium	1576:1595	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	5	14	theme	fermentation	729:740	arg1	result					715:720	a result	713:720	a result of the fermentation	713:740	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	1	15	theme	carob	158:162	arg1	extract					164:170	carob extract	158:170	carob extract	158:170	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	2	16	theme	inulinase	400:408	arg1	production					377:386	the production	373:386	the production of A. niger inulinase	373:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	3	17	theme	.7H2	590:593	arg1	O					595:595	ZnSO4 .7H2 O	584:595	ZnSO4 .7H2 O	584:595	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	18	from	consumption	1531:1541	arg1	fermentation					1558:1569	shake flask fermentation	1546:1569	shake flask fermentation	1546:1569	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	5	19	theme	invertase-type	842:855	arg1	rates					866:870	invertase-type activity rates	842:870	invertase-type activity rates	842:870	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	2	20	theme	niger	394:398	arg1	inulinase					400:408	A. niger inulinase	391:408	A. niger inulinase	391:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	8	21	theme	best-selected	1430:1442	arg1	equations					1474:1482	universal equations	1464:1482	universal equations	1464:1482	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	8	21	theme	best-selected	1430:1442	arg1	models					1444:1449	the best-selected models	1426:1449	the best-selected models	1426:1449	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	6	22	theme	consumption	1116:1126	arg1	data					1128:1131	sugar consumption data	1110:1131	sugar consumption data	1110:1131	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	3	23	theme	NH4	508:510	arg1	HPO4					515:518	(NH4 )2 HPO4	507:518	(NH4 )2 HPO4	507:518	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	24	theme	extract	1582:1588	arg1	medium					1590:1595	carob extract medium	1576:1595	carob extract medium	1576:1595	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	2	25	theme	novel	336:340	arg1	source					363:368	a novel and renewable carbon source	334:368	a novel and renewable carbon source in the production of A. niger inulinase	334:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	25	theme	novel	336:340	arg1	extract					314:320	carob extract	308:320	carob extract	308:320	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	26	theme	A.	391:392	arg1	inulinase					400:408	A. niger inulinase	391:408	A. niger inulinase	391:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	6	27	theme	sugar	1110:1114	arg1	data					1128:1131	sugar consumption data	1110:1131	sugar consumption data	1110:1131	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	5	28	theme	I/S	808:810	arg1	ratio					812:816	I/S ratio	808:816	I/S ratio	808:816	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	6	29	theme	model	1201:1205	arg1	evaluation					1207:1216	the model evaluation	1197:1216	the model evaluation	1197:1216	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	1	30	theme	Aspergillus	175:185	arg1	niger					187:191	Aspergillus niger A42	175:195	Aspergillus niger A42 (ATCC 204447)	175:209	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	1	30	theme	Aspergillus	175:185	arg1	ATCC					198:201	ATCC 204447	198:208	ATCC 204447	198:208	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	0	31	theme	mathematical	25:36	arg1	modeling					38:45	mathematical modeling	25:45	mathematical modeling	25:45	Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
31581350	6	32	theme	actual	1078:1083	arg1	production					1095:1104	the actual inulinase production	1074:1104	the actual inulinase production	1074:1104	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	1	33	theme	main	98:101	arg1	objectives					103:112	The main objectives	94:112	The main objectives of the study	94:125	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	5	34	theme	sugar	881:885	arg1	rate					899:902	maximum sugar consumption rate	873:902	maximum sugar consumption rate	873:902	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	5	35	theme	maximum	819:825	arg1	inulinase-					827:836	maximum inulinase-	819:836	maximum inulinase-	819:836	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	5	36	theme	maximum	775:781	arg1	activity					798:805	maximum invertase-type activity	775:805	maximum invertase-type activity	775:805	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	1	37	theme	optimum	258:264	arg1	medium					286:291	the optimum carob extract-based medium	254:291	the optimum carob extract-based medium	254:291	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	1	38	from	fermentation	238:249	arg1	medium					286:291	the optimum carob extract-based medium	254:291	the optimum carob extract-based medium	254:291	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	3	39	theme	NH4	546:548	arg1	NO3					550:552	NH4 NO3	546:552	NH4 NO3	546:552	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	40	dep	production	1510:1519	arg1	the					1496:1498	the	1496:1498	the	1496:1498	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	3	41	theme	medium	415:420	arg1	optimization					422:433	medium optimization	415:433	medium optimization	415:433	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	1	42	theme	carob	266:270	arg1	medium					286:291	the optimum carob extract-based medium	254:291	the optimum carob extract-based medium	254:291	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	7	43	theme	carbon	1397:1402	arg1	extract					1344:1350	carob extract	1338:1350	carob extract	1338:1350	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	7	43	theme	carbon	1397:1402	arg1	source					1404:1409	a carbon source	1395:1409	a carbon source	1395:1409	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	4	44	theme	5°Bx	690:693	arg1	extract					701:707	5°Bx carob extract	690:707	5°Bx carob extract	690:707	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	6	45	theme	mathematical	1051:1062	arg1	modeling					1064:1071	mathematical modeling	1051:1071	mathematical modeling	1051:1071	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	2	46	theme	renewable	346:354	arg1	source					363:368	a novel and renewable carbon source	334:368	a novel and renewable carbon source in the production of A. niger inulinase	334:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	46	theme	renewable	346:354	arg1	extract					314:320	carob extract	308:320	carob extract	308:320	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	1	47	theme	extract-based	272:284	arg1	medium					286:291	the optimum carob extract-based medium	254:291	the optimum carob extract-based medium	254:291	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	7	48	theme	inulinase	1382:1390	arg1	production					1368:1377	the production	1364:1377	the production of inulinase	1364:1390	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	3	49	theme	MgSO4	522:526	arg1	O					533:533	MgSO4 .7H2 O	522:533	MgSO4 .7H2 O	522:533	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	2	50	theme	carbon	356:361	arg1	source					363:368	a novel and renewable carbon source	334:368	a novel and renewable carbon source in the production of A. niger inulinase	334:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	50	theme	carbon	356:361	arg1	extract					314:320	carob extract	308:320	carob extract	308:320	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	6	51	dep	evaluation	1207:1216	arg1	results					1233:1239	results	1233:1239	results	1233:1239	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	4	52	contain	contained	660:668	arg2	extract					679:685	1% yeast extract	670:685	1% yeast extract	670:685	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	4	52	contain	contained	660:668	arg2	composition					648:658	optimum medium composition	633:658	optimum medium composition	633:658	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	4	52	contain	contained	660:668	arg1	composition					648:658	optimum medium composition	633:658	optimum medium composition	633:658	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	4	52	contain	contained	660:668	arg1	extract					701:707	5°Bx carob extract	690:707	5°Bx carob extract	690:707	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	5	53	theme	sugar	909:913	arg1	yield					927:931	sugar utilization yield	909:931	sugar utilization yield	909:931	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	6	54	theme	inulinase	1085:1093	arg1	production					1095:1104	the actual inulinase production	1074:1104	the actual inulinase production	1074:1104	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	7	55	theme	first	1316:1320	arg1	this					1303:1306	this	1303:1306	this	1303:1306	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	7	55	theme	first	1316:1320	arg1	report					1322:1327	the first report	1312:1327	the first report in which carob extract was used in the production of inulinase as a carbon source	1312:1409	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	2	56	theme	carob	308:312	arg1	source					363:368	a novel and renewable carbon source	334:368	a novel and renewable carbon source in the production of A. niger inulinase	334:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	2	56	theme	carob	308:312	arg1	extract					314:320	carob extract	308:320	carob extract	308:320	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	0	57	theme	carob	52:56	arg1	extract					58:64	carob extract	52:64	carob extract	52:64	Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
31581350	4	58	theme	carob	695:699	arg1	extract					701:707	5°Bx carob extract	690:707	5°Bx carob extract	690:707	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	3	59	theme	different	442:450	arg1	O					595:595	ZnSO4 .7H2 O	584:595	ZnSO4 .7H2 O	584:595	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	peptone					571:577	peptone	571:577	peptone	571:577	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	NO3					550:552	NH4 NO3	546:552	NH4 NO3	546:552	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	O					533:533	MgSO4 .7H2 O	522:533	MgSO4 .7H2 O	522:533	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	concentration					486:498	initial sugar concentration	472:498	initial sugar concentration (°Bx)	472:504	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	KH2					536:538	KH2 PO4	536:542	KH2 PO4	536:542	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	extract					562:568	yeast extract	556:568	yeast extract	556:568	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	HPO4					515:518	(NH4 )2 HPO4	507:518	(NH4 )2 HPO4	507:518	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	59	theme	different	442:450	arg1	variables					452:460	eight different variables	436:460	eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O	436:595	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	5	60	theme	maximum	873:879	arg1	rate					899:902	maximum sugar consumption rate	873:902	maximum sugar consumption rate	873:902	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	6	61	theme	Cone	1176:1179	arg1	models					1181:1186	Baranyi and Cone models	1164:1186	Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively	1164:1286	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	5	62	theme	activity	857:864	arg1	rates					866:870	invertase-type activity rates	842:870	invertase-type activity rates	842:870	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	4	63	theme	%	671:671	arg1	extract					679:685	1% yeast extract	670:685	1% yeast extract	670:685	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	5	64	theme	consumption	887:897	arg1	rate					899:902	maximum sugar consumption rate	873:902	maximum sugar consumption rate	873:902	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	1	65	theme	study	121:125	arg1	objectives					103:112	The main objectives	94:112	The main objectives of the study	94:125	The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium.
31581350	8	66	with	modeling	1487:1494	arg1	medium					1590:1595	carob extract medium	1576:1595	carob extract medium	1576:1595	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	8	67	from	production	1510:1519	arg1	fermentation					1558:1569	shake flask fermentation	1546:1569	shake flask fermentation	1546:1569	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	5	68	theme	utilization	915:925	arg1	yield					927:931	sugar utilization yield	909:931	sugar utilization yield	909:931	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	4	69	theme	1	670:670	arg1	%					671:671	%	671:671	%	671:671	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	2	70	from	source	363:368	arg1	production					377:386	the production	373:386	the production of A. niger inulinase	373:408	In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase.
31581350	5	71	theme	maximum	747:753	arg1	1507.03 U/ml					938:949	1507.03 U/ml	938:949	1507.03 U/ml	938:949	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	5	71	theme	maximum	747:753	arg1	activity					765:772	the maximum inulinase activity	743:772	the maximum inulinase activity	743:772	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	5	72	theme	invertase-type	783:796	arg1	activity					798:805	maximum invertase-type activity	775:805	maximum invertase-type activity	775:805	As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively.
31581350	3	73	theme	yeast	556:560	arg1	extract					562:568	yeast extract	556:568	yeast extract	556:568	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	74	theme	.7H2	528:531	arg1	O					533:533	MgSO4 .7H2 O	522:533	MgSO4 .7H2 O	522:533	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	75	theme	shake	1546:1550	arg1	fermentation					1558:1569	shake flask fermentation	1546:1569	shake flask fermentation	1546:1569	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	3	76	theme	initial	472:478	arg1	°Bx					501:503	°Bx	501:503	°Bx	501:503	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	76	theme	initial	472:478	arg1	concentration					486:498	initial sugar concentration	472:498	initial sugar concentration (°Bx)	472:504	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	77	theme	sugar	1525:1529	arg1	consumption					1531:1541	sugar consumption	1525:1541	sugar consumption	1525:1541	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	7	78	used	used	1356:1359	arg2	source					1404:1409	a carbon source	1395:1409	a carbon source	1395:1409	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	7	78	used	used	1356:1359	arg2	extract					1344:1350	carob extract	1338:1350	carob extract	1338:1350	Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source.
31581350	6	79	theme	kinetic	1259:1265	arg1	values					1267:1272	the predicted kinetic values	1245:1272	the predicted kinetic values	1245:1272	Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively.
31581350	4	80	theme	yeast	673:677	arg1	extract					679:685	1% yeast extract	670:685	1% yeast extract	670:685	After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract.
31581350	3	81	theme	sugar	480:484	arg1	°Bx					501:503	°Bx	501:503	°Bx	501:503	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	3	81	theme	sugar	480:484	arg1	concentration					486:498	initial sugar concentration	472:498	initial sugar concentration (°Bx)	472:504	For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4 )2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed.
31581350	8	82	theme	inulinase	1500:1508	arg1	production					1510:1519	inulinase production	1500:1519	inulinase production	1500:1519	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	8	83	theme	universal	1464:1472	arg1	equations					1474:1482	universal equations	1464:1482	universal equations	1464:1482	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31581350	8	83	theme	universal	1464:1472	arg1	models					1444:1449	the best-selected models	1426:1449	the best-selected models	1426:1449	Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
31139169	9	0	theme	structure	1806:1814	arg1	basis					1792:1796	the genetic basis	1780:1796	the genetic basis of O-PS structure and antigenicity	1780:1831	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	5	1	theme	D-Qui2NAc4NR	1182:1193	arg1	linkage					1171:1177	the linkage	1167:1177	the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1,	1167:1265	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	5	1	theme	D-Qui2NAc4NR	1182:1193	arg1	difference					1290:1299	the only structural difference	1270:1299	the only structural difference between O-PS from these strains	1270:1331	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	8	2	theme	identical	1593:1601	arg1	loci					1588:1591	O-PS loci	1583:1591	O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1	1583:1643	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	9	3	theme	serological	1946:1956	arg1	studies					1958:1964	serological studies	1946:1964	serological studies of this important fish pathogen	1946:1996	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	1	4	theme	bacterial	284:292	arg1	disease					305:311	bacterial cold-water disease	284:311	bacterial cold-water disease	284:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	0	5	theme	O-Polysaccharides	107:123	arg1	Characterization					37:52	Comparative Structural and Antigenic Characterization	0:52	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.	0:124	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	5	6	theme	magnetic	1142:1149	arg1	resonance					1151:1159	high-resolution nuclear magnetic resonance	1118:1159	high-resolution nuclear magnetic resonance	1118:1159	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	2	7	dep	surface	506:512	arg1	lipopolysaccharide					514:531	lipopolysaccharide	514:531	cell surface lipopolysaccharide (LPS)	501:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	2	7	dep	surface	506:512	arg1	LPS					534:536	LPS	534:536	LPS	534:536	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	5	8	theme	resonance	1151:1159	arg1	results					1073:1079	results	1073:1079	results of glycosyl composition analysis and high-resolution nuclear magnetic resonance	1073:1159	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	1	9	theme	cold-water	294:303	arg1	disease					305:311	bacterial cold-water disease	284:311	bacterial cold-water disease	284:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	9	10	theme	antigenicity	1820:1831	arg1	basis					1792:1796	the genetic basis	1780:1796	the genetic basis of O-PS structure and antigenicity	1780:1831	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	3	11	theme	repeating	774:782	arg1	unit					784:787	the repeating unit	770:787	the repeating unit of Fp CSF259-93 O-PS	770:808	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	3	11	theme	repeating	774:782	arg1	trisaccharide					542:554	A trisaccharide	540:554	A trisaccharide	540:554	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	2	12	theme	different	336:344	arg1	O-serotypes					358:368	different heat-stable O-serotypes	336:368	different heat-stable O-serotypes among strains of this gram-negative pathogen	336:413	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	4	13	theme	O-PS	1037:1040	arg1	chains					1042:1047	O-PS chains	1037:1047	O-PS chains	1037:1047	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	3	14	theme	O-PS	805:808	arg1	unit					784:787	the repeating unit	770:787	the repeating unit of Fp CSF259-93 O-PS	770:808	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	3	14	theme	O-PS	805:808	arg1	trisaccharide					542:554	A trisaccharide	540:554	A trisaccharide	540:554	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	4	15	theme	gene	835:838	arg1	cluster					840:846	the O-PS gene cluster	826:846	the O-PS gene cluster of this strain	826:861	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	4	15	theme	gene	835:838	arg1	identical					935:943	identical	935:943	identical	935:943	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	8	16	theme	Fp	1615:1616	arg1	CSF259-93					1618:1626	Fp CSF259-93	1615:1626	Fp CSF259-93	1615:1626	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	4	17	theme	trisaccharide	1010:1022	arg1	repeats					1024:1030	trisaccharide repeats	1010:1030	trisaccharide repeats	1010:1030	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	8	18	theme	O-PS	1583:1586	arg1	loci					1588:1591	O-PS loci	1583:1591	O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1	1583:1643	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	7	19	theme	purified	1480:1487	arg1	O-PS					1489:1492	O-PS	1489:1492	O-PS	1489:1492	psychrophilum antibody with purified O-PS and LPS.
31139169	2	20	theme	structural	438:447	arg1	variations					449:458	structural variations	438:458	structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS)	438:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	8	21	from	differences	1523:1533	arg1	antigenicity					1538:1549	antigenicity	1538:1549	antigenicity	1538:1549	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	3	22	theme	Fp	792:793	arg1	O-PS					805:808	Fp CSF259-93 O-PS	792:808	Fp CSF259-93 O-PS	792:808	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	6	23	dep	rabbit	1427:1432	arg1	anti-F					1444:1449	anti-F	1444:1449	anti-F	1444:1449	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	9	24	theme	fish	1984:1987	arg1	pathogen					1989:1996	this important fish pathogen	1969:1996	this important fish pathogen	1969:1996	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	8	25	with	strains	1570:1576	arg1	loci					1588:1591	O-PS loci	1583:1591	O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1	1583:1643	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	0	26	theme	Structural	12:21	arg1	Characterization					37:52	Comparative Structural and Antigenic Characterization	0:52	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.	0:124	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	3	27	theme	CSF259-93	795:803	arg1	O-PS					805:808	Fp CSF259-93 O-PS	792:808	Fp CSF259-93 O-PS	792:808	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	4	28	theme	different	909:917	arg1	O-serotype					919:928	a different O-serotype	907:928	a different O-serotype	907:928	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	5	29	from	strains	1325:1331	arg1	linkage					1171:1177	the linkage	1167:1177	the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1,	1167:1265	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	5	29	from	strains	1325:1331	arg1	difference					1290:1299	the only structural difference	1270:1299	the only structural difference between O-PS from these strains	1270:1331	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	9	30	theme	pathogen	1989:1996	arg1	studies					1958:1964	serological studies	1946:1964	serological studies of this important fish pathogen	1946:1996	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	1	31	theme	psychrophilum	225:237	arg1	strains					199:205	strains	199:205	strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease	199:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	0	32	theme	Comparative	0:10	arg1	Characterization					37:52	Comparative Structural and Antigenic Characterization	0:52	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.	0:124	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	9	33	theme	O-PS	1801:1804	arg1	structure					1806:1814	O-PS structure	1801:1814	O-PS structure	1801:1814	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	0	34	theme	Antigenic	27:35	arg1	Characterization					37:52	Comparative Structural and Antigenic Characterization	0:52	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.	0:124	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	5	35	theme	Fp	1223:1224	arg1	CSF259-93					1226:1234	Fp CSF259-93	1223:1234	Fp CSF259-93	1223:1234	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	2	36	theme	O-serotypes	358:368	arg1	identification					318:331	The identification	314:331	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen	314:413	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	2	37	theme	O-PS	485:488	arg1	moiety					491:496	the O-polysaccharide (O-PS) moiety	463:496	the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS)	463:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	9	38	theme	F.	1868:1869	arg1	O-serotypes					1885:1895	F. psychrophilum O-serotypes	1868:1895	F. psychrophilum O-serotypes	1868:1895	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	8	39	theme	LPS-based	1513:1521	arg1	differences					1523:1533	LPS-based differences	1513:1533	LPS-based differences in antigenicity	1513:1549	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	5	40	theme	high-resolution	1118:1132	arg1	resonance					1151:1159	high-resolution nuclear magnetic resonance	1118:1159	high-resolution nuclear magnetic resonance	1118:1159	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	2	41	theme	heat-stable	346:356	arg1	O-serotypes					358:368	different heat-stable O-serotypes	336:368	different heat-stable O-serotypes among strains of this gram-negative pathogen	336:413	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	9	42	theme	important	1974:1982	arg1	pathogen					1989:1996	this important fish pathogen	1969:1996	this important fish pathogen	1969:1996	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	9	43	theme	psychrophilum	1871:1883	arg1	O-serotypes					1885:1895	F. psychrophilum O-serotypes	1868:1895	F. psychrophilum O-serotypes	1868:1895	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	2	44	theme	gram-negative	392:404	arg1	pathogen					406:413	this gram-negative pathogen	387:413	this gram-negative pathogen	387:413	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	4	45	theme	putative	979:986	arg1	wzy					956:958	wzy	956:958	wzy	956:958	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	4	45	theme	putative	979:986	arg1	polymerase					988:997	the putative polymerase	975:997	the putative polymerase that links trisaccharide repeats into O-PS chains	975:1047	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	5	46	theme	nuclear	1134:1140	arg1	resonance					1151:1159	high-resolution nuclear magnetic resonance	1118:1159	high-resolution nuclear magnetic resonance	1118:1159	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	2	47	theme	O-polysaccharide	467:482	arg1	moiety					491:496	the O-polysaccharide (O-PS) moiety	463:496	the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS)	463:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	9	48	theme	O-serotypes	1885:1895	arg1	O-serotypes					1885:1895	F. psychrophilum O-serotypes	1868:1895	F. psychrophilum O-serotypes	1868:1895	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	9	48	theme	O-serotypes	1885:1895	arg1	repertoire					1854:1863	the repertoire	1850:1863	the repertoire of F. psychrophilum O-serotypes	1850:1895	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	6	49	theme	trout	1438:1442	arg1	reactions					1414:1422	the reactions	1410:1422	the reactions of rabbit and trout anti-F	1410:1449	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	6	50	theme	O-serotype	1366:1375	arg1	specificity					1377:1387	O-serotype specificity	1366:1387	O-serotype specificity	1366:1387	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	1	51	dep	trout	261:265	arg1	syndrome					271:278	fry syndrome	267:278	fry syndrome	267:278	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	1	51	dep	trout	261:265	arg1	disease					305:311	bacterial cold-water disease	284:311	bacterial cold-water disease	284:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	4	52	theme	Fp	875:876	arg1	950106-1/1					878:887	Fp 950106-1/1	875:887	Fp 950106-1/1	875:887	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	1	53	theme	rainbow	253:259	arg1	trout					261:265	rainbow trout fry syndrome and bacterial cold-water disease	253:311	rainbow trout fry syndrome and bacterial cold-water disease	253:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	0	54	theme	Distinct	69:76	arg1	O-Polysaccharides					107:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	8	55	theme	Fp	1631:1632	arg1	950106-1/1					1634:1643	Fp 950106-1/1	1631:1643	Fp 950106-1/1	1631:1643	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	8	56	from	synthesis	1687:1695	arg1	Qui2NAc4NR					1722:1731	Qui2NAc4NR	1722:1731	Qui2NAc4NR	1722:1731	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	3	57	theme	dihydroxyhexanamido	709:727	arg1	derivative					729:738	a dihydroxyhexanamido derivative	707:738	a dihydroxyhexanamido derivative	707:738	A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of Fp CSF259-93 O-PS.
31139169	9	58	theme	genetic	1784:1790	arg1	basis					1792:1796	the genetic basis	1780:1796	the genetic basis of O-PS structure and antigenicity	1780:1831	The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of F. psychrophilum O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
31139169	2	59	theme	pathogen	406:413	arg1	strains					376:382	strains	376:382	strains of this gram-negative pathogen	376:413	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	2	60	theme	surface	506:512	arg1	moiety					491:496	the O-polysaccharide (O-PS) moiety	463:496	the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS)	463:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	5	61	theme	glycosyl	1084:1091	arg1	analysis					1105:1112	glycosyl composition analysis	1084:1112	glycosyl composition analysis	1084:1112	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	1	62	theme	underlying	152:161	arg1	basis					163:167	the underlying basis	148:167	the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease	148:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	8	63	theme	different	1700:1708	arg1	R-groups					1710:1717	different R-groups	1700:1717	different R-groups	1700:1717	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	6	64	from	difference	1352:1361	arg1	specificity					1377:1387	O-serotype specificity	1366:1387	O-serotype specificity	1366:1387	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	6	65	theme	corresponding	1338:1350	arg1	difference					1352:1361	The corresponding difference	1334:1361	The corresponding difference in O-serotype specificity	1334:1387	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	5	66	theme	composition	1093:1103	arg1	analysis					1105:1112	glycosyl composition analysis	1084:1112	glycosyl composition analysis	1084:1112	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	5	67	theme	Fp	1252:1253	arg1	950106-1/1					1255:1264	Fp 950106-1/1	1252:1264	Fp 950106-1/1	1252:1264	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	2	68	from	variations	449:458	arg1	moiety					491:496	the O-polysaccharide (O-PS) moiety	463:496	the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS)	463:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	4	69	theme	O-PS	830:833	arg1	cluster					840:846	the O-PS gene cluster	826:846	the O-PS gene cluster of this strain	826:861	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	4	69	theme	O-PS	830:833	arg1	identical					935:943	identical	935:943	identical	935:943	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	1	70	theme	trout	261:265	arg1	psychrophilum					225:237	Flavobacterium psychrophilum	210:237	Flavobacterium psychrophilum	210:237	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	1	70	theme	trout	261:265	arg1	agent					244:248	the agent	240:248	the agent of rainbow trout fry syndrome and bacterial cold-water disease	240:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	5	71	theme	analysis	1105:1112	arg1	results					1073:1079	results	1073:1079	results of glycosyl composition analysis and high-resolution nuclear magnetic resonance	1073:1159	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	4	72	theme	strain	856:861	arg1	cluster					840:846	the O-PS gene cluster	826:846	the O-PS gene cluster of this strain	826:861	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	4	72	theme	strain	856:861	arg1	identical					935:943	identical	935:943	identical	935:943	Interestingly, the O-PS gene cluster of this strain and that of Fp 950106-1/1, which belongs to a different O-serotype, are identical except for wzy, which encodes the putative polymerase that links trisaccharide repeats into O-PS chains.
31139169	1	73	theme	specificity	181:191	arg1	basis					163:167	the underlying basis	148:167	the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease	148:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	1	74	theme	fry	267:269	arg1	syndrome					271:278	fry syndrome	267:278	fry syndrome	267:278	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	0	75	theme	psychrophilum	93:105	arg1	O-Polysaccharides					107:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	7	76	with	antibody	1466:1473	arg1	O-PS					1489:1492	O-PS	1489:1492	O-PS	1489:1492	psychrophilum antibody with purified O-PS and LPS.
31139169	7	76	with	antibody	1466:1473	arg1	LPS					1498:1500	LPS	1498:1500	LPS	1498:1500	psychrophilum antibody with purified O-PS and LPS.
31139169	6	77	theme	rabbit	1427:1432	arg1	reactions					1414:1422	the reactions	1410:1422	the reactions of rabbit and trout anti-F	1410:1449	The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti-F.
31139169	1	78	theme	serotype	172:179	arg1	specificity					181:191	serotype specificity	172:191	serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease	172:311	Little is known about the underlying basis of serotype specificity among strains of Flavobacterium psychrophilum, the agent of rainbow trout fry syndrome and bacterial cold-water disease.
31139169	8	79	theme	R-groups	1710:1717	arg1	synthesis					1687:1695	synthesis	1687:1695	synthesis of different R-groups in Qui2NAc4NR	1687:1731	Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of Fp CSF259-93 or Fp 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR.
31139169	0	80	theme	Flavobacterium	78:91	arg1	O-Polysaccharides					107:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides	57:123	Comparative Structural and Antigenic Characterization of Genetically Distinct Flavobacterium psychrophilum O-Polysaccharides.
31139169	2	81	theme	cell	501:504	arg1	surface					506:512	cell surface lipopolysaccharide (LPS)	501:537	cell surface lipopolysaccharide (LPS)	501:537	The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the O-polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS).
31139169	5	82	theme	structural	1279:1288	arg1	linkage					1171:1177	the linkage	1167:1177	the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1,	1167:1265	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
31139169	5	82	theme	structural	1279:1288	arg1	difference					1290:1299	the only structural difference	1270:1299	the only structural difference between O-PS from these strains	1270:1331	We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for Fp CSF259-93 versus β1-3 for Fp 950106-1/1, is the only structural difference between O-PS from these strains.
29805006	0	0	from	composition	17:27	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	7	1	theme	polysaccharides	981:995	arg1	linkages					965:972	the major structural linkages	944:972	the major structural linkages of the polysaccharides of both ori and mut EPSs	944:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	0	2	from	structure	30:38	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	3	3	dep	protein	535:541	arg1	w/w					552:554	w/w	552:554	w/w	552:554	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	3	dep	protein	535:541	arg1	%					549:549	25-40%	544:549	25-40%	544:549	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	6	4	theme	EPSs	876:879	arg1	EPSs					876:879	EPSs	876:879	EPSs	876:879	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	6	4	theme	EPSs	876:879	arg1	structure					841:849	structure	841:849	structure	841:849	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	6	4	theme	EPSs	876:879	arg1	groups					866:871	functional groups	855:871	functional groups	855:871	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	0	5	theme	substances	83:92	arg1	composition					17:27	composition	17:27	composition	17:27	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	5	theme	substances	83:92	arg1	bioactivity					44:54	bioactivity	44:54	bioactivity	44:54	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	5	theme	substances	83:92	arg1	structure					30:38	structure	30:38	structure	30:38	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	8	6	theme	lung	1246:1249	arg1	carcinoma					1251:1259	human lung carcinoma A549	1240:1264	human lung carcinoma A549	1240:1264	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	5	7	dep	%	789:789	arg1	w/w					792:794	w/w	792:794	w/w	792:794	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	5	7	dep	%	789:789	arg1	basis					801:805	dry basis	797:805	dry basis	797:805	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	3	8	theme	dry	520:522	arg1	w/w					515:517	w/w	515:517	w/w	515:517	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	8	theme	dry	520:522	arg1	basis					524:528	dry basis	520:528	dry basis	520:528	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	5	9	theme	polysaccharide	821:834	arg1	polysaccharide					821:834	the total polysaccharide	811:834	the total polysaccharide	811:834	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	5	9	theme	polysaccharide	821:834	arg1	%					789:789	up to 82-90%	778:789	up to 82-90% (w/w, dry basis) of the total polysaccharide	778:834	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	3	10	dep	morphology	592:601	arg1	study					603:607	study	603:607	study	603:607	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	0	11	theme	original	99:106	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	8	12	theme	cervical	1211:1218	arg1	HeLa					1230:1233	human cervical carcinoma HeLa	1205:1233	human cervical carcinoma HeLa	1205:1233	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	8	13	theme	human	1205:1209	arg1	HeLa					1230:1233	human cervical carcinoma HeLa	1205:1233	human cervical carcinoma HeLa	1205:1233	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	6	14	theme	functional	855:864	arg1	EPSs					876:879	EPSs	876:879	EPSs	876:879	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	6	14	theme	functional	855:864	arg1	groups					866:871	functional groups	855:871	functional groups	855:871	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	0	15	from	bioactivity	44:54	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	7	16	theme	structural	954:963	arg1	linkages					965:972	the major structural linkages	944:972	the major structural linkages of the polysaccharides of both ori and mut EPSs	944:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	4	17	theme	EPS	656:658	arg1	polysaccharide					660:673	the EPS polysaccharide	652:673	the EPS polysaccharide	652:673	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	3	18	dep	contained	481:489	arg1	shown					579:583	shown	579:583	was shown by the morphology study with an AFM	575:619	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	9	19	theme	anti-inflammatory	1304:1320	arg1	activities					1322:1331	antioxidant and anti-inflammatory activities	1288:1331	antioxidant and anti-inflammatory activities	1288:1331	The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.
29805006	0	20	theme	stress-induced	112:125	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	4	21	theme	xylose	743:748	arg1	arabinose					712:720	arabinose	712:720	arabinose	712:720	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	4	21	theme	xylose	743:748	arg1	amount					733:738	trace amount	727:738	trace amount of xylose	727:748	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	4	21	theme	xylose	743:748	arg1	galactose					701:709	galactose	701:709	galactose	701:709	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	4	21	theme	xylose	743:748	arg1	glucose					692:698	glucose	692:698	glucose	692:698	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	4	21	theme	xylose	743:748	arg1	xylose					743:748	xylose	743:748	xylose	743:748	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	3	22	from	strains	466:472	arg1	EPSs					451:454	The EPSs	447:454	The EPSs from both strains	447:472	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	23	dep	polysaccharide	491:504	arg1	w/w					515:517	w/w	515:517	w/w	515:517	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	23	dep	polysaccharide	491:504	arg1	%					512:512	41-64%	507:512	41-64%	507:512	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	24	contain	contained	481:489	arg1	EPSs					451:454	The EPSs	447:454	The EPSs from both strains	447:472	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	24	contain	contained	481:489	arg2	protein					535:541	protein	535:541	protein (25-40%, w/w, dry basis)	535:566	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	24	contain	contained	481:489	arg2	polysaccharide					491:504	polysaccharide	491:504	polysaccharide (41-64%, w/w, dry basis)	491:529	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	2	25	theme	striatum	333:340	arg1	strains					319:325	Two strains	315:325	Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress)	315:430	Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared.
29805006	3	26	with	study	603:607	arg1	AFM					617:619	an AFM	614:619	an AFM	614:619	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	8	27	theme	anti-tumor	1120:1129	arg1	activities					1131:1140	anti-tumor activities	1120:1140	anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549	1120:1264	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	7	28	theme	mut	1013:1015	arg1	polysaccharides					981:995	the polysaccharides	977:995	the polysaccharides of both ori and mut EPSs	977:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	7	29	theme	major	948:952	arg1	linkages					965:972	the major structural linkages	944:972	the major structural linkages of the polysaccharides of both ori and mut EPSs	944:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	9	30	theme	antioxidant	1288:1298	arg1	activities					1322:1331	antioxidant and anti-inflammatory activities	1288:1331	antioxidant and anti-inflammatory activities	1288:1331	The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.
29805006	8	31	contain	have	1115:1118	arg1	EPSs					1096:1099	The EPSs	1092:1099	The EPSs	1092:1099	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	8	31	contain	have	1115:1118	arg2	activities					1131:1140	anti-tumor activities	1120:1140	anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549	1120:1264	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	1	32	theme	extracellular	199:211	arg1	substances					223:232	extracellular polymeric substances	199:232	extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities	199:312	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	1	32	theme	extracellular	199:211	arg1	EPSs					235:238	EPSs	235:238	EPSs	235:238	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	0	33	theme	Thraustochytrium	138:153	arg1	striatum					155:162	Thraustochytrium striatum	138:162	Thraustochytrium striatum	138:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	5	34	theme	total	815:819	arg1	polysaccharide					821:834	the total polysaccharide	811:834	the total polysaccharide	811:834	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	1	35	theme	polymeric	213:221	arg1	substances					223:232	extracellular polymeric substances	199:232	extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities	199:312	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	1	35	theme	polymeric	213:221	arg1	EPSs					235:238	EPSs	235:238	EPSs	235:238	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	0	36	theme	composition	17:27	arg1	Investigation					0:12	Investigation	0:12	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.	0:163	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	4	37	theme	polysaccharide	660:673	arg1	profile					641:647	The monosaccharide profile	622:647	The monosaccharide profile of the EPS polysaccharide	622:673	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	2	38	theme	high-nitrogen	410:422	arg1	stress					424:429	high-nitrogen stress	410:429	high-nitrogen stress	410:429	Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared.
29805006	7	39	theme	NMR	918:920	arg1	results					922:928	The NMR results	914:928	The NMR results	914:928	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	4	40	theme	trace	727:731	arg1	xylose					743:748	xylose	743:748	xylose	743:748	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	4	40	theme	trace	727:731	arg1	amount					733:738	trace amount	727:738	trace amount of xylose	727:748	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	9	41	theme	antibacterial	1337:1349	arg1	activity					1351:1358	antibacterial activity	1337:1358	antibacterial activity against Pseudomonas aeruginosa	1337:1389	The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.
29805006	0	42	theme	structure	30:38	arg1	Investigation					0:12	Investigation	0:12	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.	0:163	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	43	theme	striatum	155:162	arg1	strains					127:133	original and stress-induced strains	99:133	original and stress-induced strains of Thraustochytrium striatum	99:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	8	44	theme	mouse	1150:1154	arg1	melanoma					1156:1163	mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549	1150:1264	melanoma	1156:1163	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	8	45	theme	human	1240:1244	arg1	carcinoma					1251:1259	human lung carcinoma A549	1240:1264	human lung carcinoma A549	1240:1264	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	4	46	theme	monosaccharide	626:639	arg1	profile					641:647	The monosaccharide profile	622:647	The monosaccharide profile of the EPS polysaccharide	622:673	The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose.
29805006	0	47	theme	bioactivity	44:54	arg1	Investigation					0:12	Investigation	0:12	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.	0:163	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	48	from	strains	127:133	arg1	composition					17:27	composition	17:27	composition	17:27	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	48	from	strains	127:133	arg1	substances					83:92	extracellular polymeric substances	59:92	extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum	59:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	48	from	strains	127:133	arg1	bioactivity					44:54	bioactivity	44:54	bioactivity	44:54	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	0	48	from	strains	127:133	arg1	structure					30:38	structure	30:38	structure	30:38	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	7	49	dep	ori	1005:1007	arg1	EPSs					1017:1020	EPSs	1017:1020	EPSs	1017:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	5	50	theme	dry	797:799	arg1	w/w					792:794	w/w	792:794	w/w	792:794	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	5	50	theme	dry	797:799	arg1	basis					801:805	dry basis	797:805	dry basis	797:805	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	7	51	theme	ori	1005:1007	arg1	polysaccharides					981:995	the polysaccharides	977:995	the polysaccharides of both ori and mut EPSs	977:1020	The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose.
29805006	1	52	from	substances	223:232	arg1	composition					273:283	composition	273:283	composition	273:283	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	1	52	from	substances	223:232	arg1	bioactivities					300:312	bioactivities	300:312	bioactivities	300:312	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	1	52	from	substances	223:232	arg1	structure					286:294	structure	286:294	structure	286:294	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	8	53	theme	prostate	1179:1186	arg1	carcinoma					1188:1196	human prostate carcinoma DU145	1173:1202	human prostate carcinoma DU145	1173:1202	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	5	54	dep	82-90	784:788	arg1	to					781:782	to	781:782	to	781:782	Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide.
29805006	8	55	theme	carcinoma	1220:1228	arg1	HeLa					1230:1233	human cervical carcinoma HeLa	1205:1233	human cervical carcinoma HeLa	1205:1233	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	8	56	dep	melanoma	1156:1163	arg1	B16-F0					1165:1170	B16-F0	1165:1170	B16-F0	1165:1170	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	8	57	theme	human	1173:1177	arg1	carcinoma					1188:1196	human prostate carcinoma DU145	1173:1202	human prostate carcinoma DU145	1173:1202	The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549.
29805006	0	58	theme	polymeric	73:81	arg1	substances					83:92	extracellular polymeric substances	59:92	extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum	59:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	6	59	dep	structure	841:849	arg1	The					837:839	The	837:839	The	837:839	The structure and functional groups of EPSs were determined by FTIR and NMR.
29805006	0	60	theme	extracellular	59:71	arg1	substances					83:92	extracellular polymeric substances	59:92	extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum	59:162	Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.
29805006	3	61	theme	dry	557:559	arg1	w/w					552:554	w/w	552:554	w/w	552:554	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	3	61	theme	dry	557:559	arg1	basis					561:565	dry basis	557:565	dry basis	557:565	The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM.
29805006	2	62	theme	high-biomass	371:382	arg1	strains					390:396	original (ori) and high-biomass (mut) strains	352:396	strains	390:396	Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared.
29805006	2	62	theme	high-biomass	371:382	arg1	mut					385:387	mut	385:387	mut	385:387	Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared.
29805006	1	63	theme	striatum	261:268	arg1	substances					223:232	extracellular polymeric substances	199:232	extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities	199:312	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29805006	1	63	theme	striatum	261:268	arg1	EPSs					235:238	EPSs	235:238	EPSs	235:238	This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities.
29974437	5	0	from	shoots	714:719	arg1	hybrids					753:759	both hybrids	748:759	both hybrids	748:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	3	1	theme	shoot	426:430	arg1	walls					437:441	shoot cell walls	426:441	shoot cell walls	426:441	Changes in the composition of shoot cell walls were observed.
29974437	2	2	from	effect	241:246	arg1	parameters					282:291	shoot physiological parameters	262:291	shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content)	262:353	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	4	3	theme	hemicelluloses	496:509	arg1	hemicelluloses					496:509	hemicelluloses	496:509	hemicelluloses	496:509	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	4	3	theme	hemicelluloses	496:509	arg1	amount					486:491	the amount	482:491	the amount of hemicelluloses in shoot cell walls	482:529	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	5	4	theme	cell	691:694	arg1	structure					701:709	the cell wall structure	687:709	the cell wall structure of shoots, compared to the roots, in both hybrids	687:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	4	5	located	observed	634:641	arg1	Almansa					659:665	the tolerant Almansa	646:665	the tolerant Almansa	646:665	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	4	5	located	observed	634:641	arg2	opposite					621:628	opposite	621:628	opposite	621:628	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	7	6	theme	Cd2+	980:983	arg1	amount					985:990	the highest Cd2+ amount	968:990	the highest Cd2+ amount	968:990	Nevertheless, in both hybrids, the highest Cd2+ amount was found in hemicelluloses.
29974437	1	7	dep	walls	75:79	arg1	both					165:168	both	165:168	both	165:168	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	8	8	theme	wall	1122:1125	arg1	permeability					1127:1138	lower cell wall permeability	1111:1138	lower cell wall permeability	1111:1138	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	5	9	theme	hemicelluloses	823:836	arg1	fractions					838:846	hemicelluloses fractions	823:846	hemicelluloses fractions	823:846	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	8	10	theme	walls	1051:1055	arg1	modification					1026:1037	Such modification	1021:1037	Such modification of the cell walls	1021:1055	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	5	11	from	structure	701:709	arg1	hybrids					753:759	both hybrids	748:759	both hybrids	748:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	3	12	theme	walls	437:441	arg1	composition					411:421	the composition	407:421	the composition of shoot cell walls	407:441	Changes in the composition of shoot cell walls were observed.
29974437	2	13	theme	smaller	233:239	arg1	effect					241:246	a smaller effect	231:246	a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content)	231:353	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	7	14	located	found	996:1000	arg1	hybrids					959:965	both hybrids	954:965	both hybrids	954:965	Nevertheless, in both hybrids, the highest Cd2+ amount was found in hemicelluloses.
29974437	7	14	located	found	996:1000	arg1	hemicelluloses					1005:1018	hemicelluloses	1005:1018	hemicelluloses	1005:1018	Nevertheless, in both hybrids, the highest Cd2+ amount was found in hemicelluloses.
29974437	7	14	located	found	996:1000	arg2	amount					985:990	the highest Cd2+ amount	968:990	the highest Cd2+ amount	968:990	Nevertheless, in both hybrids, the highest Cd2+ amount was found in hemicelluloses.
29974437	4	15	theme	shoot	514:518	arg1	walls					525:529	shoot cell walls	514:529	shoot cell walls	514:529	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	4	16	theme	tolerant	650:657	arg1	Almansa					659:665	the tolerant Almansa	646:665	the tolerant Almansa	646:665	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	8	17	theme	pollutant	1168:1176	arg1	influx					1178:1183	a lower pollutant influx	1160:1183	a lower pollutant influx into the protoplast	1160:1203	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	2	18	theme	shoot	262:266	arg1	parameters					282:291	shoot physiological parameters	262:291	shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content)	262:353	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	5	19	theme	total	806:810	arg1	lignin					812:817	total lignin	806:817	total lignin	806:817	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	4	20	from	amount	486:491	arg1	walls					525:529	shoot cell walls	514:529	shoot cell walls	514:529	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	2	21	theme	photosynthetic	322:335	arg1	content					346:352	photosynthetic pigments content	322:352	photosynthetic pigments content	322:352	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	2	21	theme	photosynthetic	322:335	arg1	elongation					300:309	elongation	300:309	elongation	300:309	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	5	22	theme	wall	696:699	arg1	structure					701:709	the cell wall structure	687:709	the cell wall structure of shoots, compared to the roots, in both hybrids	687:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	1	23	theme	shoot	64:68	arg1	walls					75:79	shoot cell walls	64:79	shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa,	64:163	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	24	theme	sensitive	121:129	arg1	Novania					131:137	the sensitive Novania	117:137	the sensitive Novania	117:137	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	24	theme	sensitive	121:129	arg1	hybrids					94:100	two maize hybrids	84:100	two maize hybrids (Zea mays L.)	84:114	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	0	25	theme	shoot	6:10	arg1	walls					17:21	shoot cell walls	6:21	shoot cell walls	6:21	Maize shoot cell walls under cadmium stress.
29974437	6	26	theme	lignocellulose	853:866	arg1	complex					868:874	The lignocellulose complex	849:874	The lignocellulose complex	849:874	The lignocellulose complex remained unchanged in the shoots in comparison to the roots.
29974437	7	27	theme	highest	972:978	arg1	amount					985:990	the highest Cd2+ amount	968:990	the highest Cd2+ amount	968:990	Nevertheless, in both hybrids, the highest Cd2+ amount was found in hemicelluloses.
29974437	1	28	theme	cell	70:73	arg1	walls					75:79	shoot cell walls	64:79	shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa,	64:163	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	6	29	from	shoots	902:907	arg1	comparison					912:921	comparison	912:921	comparison to the roots	912:934	The lignocellulose complex remained unchanged in the shoots in comparison to the roots.
29974437	6	29	from	shoots	902:907	arg1	unchanged					885:893	unchanged	885:893	unchanged	885:893	The lignocellulose complex remained unchanged in the shoots in comparison to the roots.
29974437	2	30	theme	cadmium	251:257	arg1	effect					241:246	a smaller effect	231:246	a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content)	231:353	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	2	31	theme	dry	312:314	arg1	elongation					300:309	elongation	300:309	elongation	300:309	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	2	31	theme	dry	312:314	arg1	mass					316:319	dry mass	312:319	dry mass	312:319	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	8	32	theme	binding	1084:1090	arg1	sites					1092:1096	binding sites	1084:1096	binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast	1084:1203	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	1	33	theme	walls	75:79	arg1	composition					49:59	The composition	45:59	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment	45:192	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	3	34	theme	cell	432:435	arg1	walls					437:441	shoot cell walls	426:441	shoot cell walls	426:441	Changes in the composition of shoot cell walls were observed.
29974437	1	35	theme	tolerant	147:154	arg1	Almansa					156:162	the tolerant Almansa	143:162	the tolerant Almansa	143:162	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	35	theme	tolerant	147:154	arg1	hybrids					94:100	two maize hybrids	84:100	two maize hybrids (Zea mays L.)	84:114	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	0	36	theme	cadmium	29:35	arg1	stress					37:42	cadmium stress	29:42	cadmium stress	29:42	Maize shoot cell walls under cadmium stress.
29974437	5	37	theme	lignin	812:817	arg1	quantities					792:801	higher quantities	785:801	higher quantities of total lignin	785:817	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	6	38	from	unchanged	885:893	arg1	shoots					902:907	the shoots	898:907	the shoots in comparison to the roots	898:934	The lignocellulose complex remained unchanged in the shoots in comparison to the roots.
29974437	8	39	theme	lower	1162:1166	arg1	influx					1178:1183	a lower pollutant influx	1160:1183	a lower pollutant influx into the protoplast	1160:1203	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	4	40	theme	lignocellulose	559:572	arg1	complex					574:580	lignocellulose complex	559:580	lignocellulose complex	559:580	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	8	41	theme	cell	1117:1120	arg1	permeability					1127:1138	lower cell wall permeability	1111:1138	lower cell wall permeability	1111:1138	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	8	42	theme	sites	1092:1096	arg1	amount					1074:1079	the amount	1070:1079	the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast	1070:1203	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	8	42	theme	sites	1092:1096	arg1	sites					1092:1096	binding sites	1084:1096	binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast	1084:1203	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	2	43	theme	pigments	337:344	arg1	content					346:352	photosynthetic pigments content	322:352	photosynthetic pigments content	322:352	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	2	43	theme	pigments	337:344	arg1	elongation					300:309	elongation	300:309	elongation	300:309	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	8	44	theme	lower	1111:1115	arg1	permeability					1127:1138	lower cell wall permeability	1111:1138	lower cell wall permeability	1111:1138	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	5	45	located	observed	766:773	arg1	quantities					792:801	higher quantities	785:801	higher quantities of total lignin	785:817	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	5	45	located	observed	766:773	arg2	Dissimilarities					668:682	Dissimilarities	668:682	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids	668:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	5	45	located	observed	766:773	arg1	fractions					838:846	hemicelluloses fractions	823:846	hemicelluloses fractions	823:846	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	1	46	theme	maize	88:92	arg1	Novania					131:137	the sensitive Novania	117:137	the sensitive Novania	117:137	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	46	theme	maize	88:92	arg1	L.					112:113	Zea mays L.	103:113	Zea mays L.	103:113	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	46	theme	maize	88:92	arg1	Almansa					156:162	the tolerant Almansa	143:162	the tolerant Almansa	143:162	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	46	theme	maize	88:92	arg1	hybrids					94:100	two maize hybrids	84:100	two maize hybrids (Zea mays L.)	84:114	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	5	47	theme	shoots	714:719	arg1	structure					701:709	the cell wall structure	687:709	the cell wall structure of shoots, compared to the roots, in both hybrids	687:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	1	48	theme	hybrids	94:100	arg1	walls					75:79	shoot cell walls	64:79	shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa,	64:163	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	2	49	theme	Previous	207:214	arg1	results					216:222	Previous results	207:222	Previous results	207:222	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	3	50	from	Changes	396:402	arg1	composition					411:421	the composition	407:421	the composition of shoot cell walls	407:441	Changes in the composition of shoot cell walls were observed.
29974437	2	51	theme	physiological	268:280	arg1	parameters					282:291	shoot physiological parameters	262:291	shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content)	262:353	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	2	52	dep	elongation	300:309	arg1	e.g.					294:297	e.g.	294:297	e.g.	294:297	Previous results showed a smaller effect of cadmium on shoot physiological parameters (e.g., elongation, dry mass, photosynthetic pigments content) in both hybrids compared to their roots.
29974437	5	53	from	Dissimilarities	668:682	arg1	structure					701:709	the cell wall structure	687:709	the cell wall structure of shoots, compared to the roots, in both hybrids	687:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	1	54	theme	Zea	103:105	arg1	L.					112:113	Zea mays L.	103:113	Zea mays L.	103:113	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	54	theme	Zea	103:105	arg1	hybrids					94:100	two maize hybrids	84:100	two maize hybrids (Zea mays L.)	84:114	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	4	55	theme	cell	520:523	arg1	walls					525:529	shoot cell walls	514:529	shoot cell walls	514:529	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	5	56	theme	higher	785:790	arg1	quantities					792:801	higher quantities	785:801	higher quantities of total lignin	785:817	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	1	57	theme	mays	107:110	arg1	L.					112:113	Zea mays L.	103:113	Zea mays L.	103:113	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	1	57	theme	mays	107:110	arg1	hybrids					94:100	two maize hybrids	84:100	two maize hybrids (Zea mays L.)	84:114	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	4	58	theme	complex	574:580	arg1	amount					549:554	the amount	545:554	the amount of lignocellulose complex	545:580	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	4	58	theme	complex	574:580	arg1	complex					574:580	lignocellulose complex	559:580	lignocellulose complex	559:580	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	1	59	theme	cadmium	176:182	arg1	treatment					184:192	cadmium treatment	176:192	cadmium treatment	176:192	The composition of shoot cell walls of two maize hybrids (Zea mays L.), the sensitive Novania and the tolerant Almansa, both after cadmium treatment was studied.
29974437	8	60	theme	Such	1021:1024	arg1	modification					1026:1037	Such modification	1021:1037	Such modification of the cell walls	1021:1055	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29974437	0	61	theme	cell	12:15	arg1	walls					17:21	shoot cell walls	6:21	shoot cell walls	6:21	Maize shoot cell walls under cadmium stress.
29974437	5	62	from	hybrids	753:759	arg1	structure					701:709	the cell wall structure	687:709	the cell wall structure of shoots, compared to the roots, in both hybrids	687:759	Dissimilarities in the cell wall structure of shoots, compared to the roots, in both hybrids were observed mainly in higher quantities of total lignin, in hemicelluloses fractions.
29974437	4	63	theme	sensitive	599:607	arg1	hybrid					609:614	the sensitive hybrid	595:614	the sensitive hybrid	595:614	It was ascertained that the amount of hemicelluloses in shoot cell walls decreased and the amount of lignocellulose complex increased in the sensitive hybrid; the opposite was observed in the tolerant Almansa.
29974437	8	64	theme	cell	1046:1049	arg1	walls					1051:1055	the cell walls	1042:1055	the cell walls	1042:1055	Such modification of the cell walls might affect the amount of binding sites resulting in lower cell wall permeability and subsequently in a lower pollutant influx into the protoplast.
29039004	1	0	theme	important	176:184	arg1	tool					186:189	an important tool	173:189	an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides	173:267	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	3	1	theme	Brachypodium	511:522	arg1	plants					524:529	Brachypodium plants	511:529	Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	511:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	3	2	theme	biosynthesis	598:609	arg1	pathway					611:617	xyloglucan biosynthesis pathway	587:617	xyloglucan biosynthesis pathway in Brachypodium distachyon	587:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	1	3	theme	Matrix-assisted	70:84	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	3	theme	Matrix-assisted	70:84	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	3	4	from	distachyon	635:644	arg1	characterization					567:582	functional characterization	556:582	functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	556:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	1	5	theme	MALDI-TOF	148:156	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	5	theme	MALDI-TOF	148:156	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	3	6	theme	xyloglucan	459:468	arg1	information					496:506	xyloglucan composition and structure information	459:506	xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	459:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	1	7	theme	laser	86:90	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	7	theme	laser	86:90	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	8	theme	desorption-ionization	92:112	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	8	theme	desorption-ionization	92:112	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	3	9	theme	mass	430:433	arg1	spectrometry					435:446	mass spectrometry	430:446	mass spectrometry	430:446	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	0	10	from	Analysis	33:40	arg1	distachyon					58:67	Brachypodium distachyon	45:67	Brachypodium distachyon	45:67	Methods for Xyloglucan Structure Analysis in Brachypodium distachyon.
29039004	1	11	theme	time-of-flight	114:127	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	11	theme	time-of-flight	114:127	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	2	12	theme	polysaccharide	370:383	arg1	structures					385:394	cell wall polysaccharide structures	360:394	cell wall polysaccharide structures	360:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	13	theme	rapid	309:313	arg1	technique					275:283	This technique	270:283	This technique	270:283	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	13	theme	rapid	309:313	arg1	means					340:344	a rapid, sensitive, and accurate means	307:344	a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures	307:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	14	theme	wall	365:368	arg1	structures					385:394	cell wall polysaccharide structures	360:394	cell wall polysaccharide structures	360:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	0	15	theme	Structure	23:31	arg1	Analysis					33:40	Xyloglucan Structure Analysis	12:40	Xyloglucan Structure Analysis in Brachypodium distachyon	12:67	Methods for Xyloglucan Structure Analysis in Brachypodium distachyon.
29039004	3	16	theme	functional	556:565	arg1	characterization					567:582	functional characterization	556:582	functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	556:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	3	17	from	pathway	611:617	arg1	distachyon					635:644	Brachypodium distachyon	622:644	Brachypodium distachyon	622:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	1	18	theme	biomolecules	211:222	arg1	analysis					199:206	the analysis	195:206	the analysis of biomolecules, such as DNA, peptides, and oligosaccharides	195:267	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	19	theme	mass	129:132	arg1	spectrometry					134:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry	70:145	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)	70:160	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	1	19	theme	mass	129:132	arg1	MS					158:159	MALDI-TOF MS	148:159	MALDI-TOF MS	148:159	Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides.
29039004	3	20	theme	plants	524:529	arg1	information					496:506	xyloglucan composition and structure information	459:506	xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	459:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	3	21	theme	Brachypodium	622:633	arg1	distachyon					635:644	Brachypodium distachyon	622:644	Brachypodium distachyon	622:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	2	22	theme	accurate	331:338	arg1	technique					275:283	This technique	270:283	This technique	270:283	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	22	theme	accurate	331:338	arg1	means					340:344	a rapid, sensitive, and accurate means	307:344	a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures	307:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	3	23	theme	structure	486:494	arg1	information					496:506	xyloglucan composition and structure information	459:506	xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	459:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	0	24	theme	Brachypodium	45:56	arg1	distachyon					58:67	Brachypodium distachyon	45:67	Brachypodium distachyon	45:67	Methods for Xyloglucan Structure Analysis in Brachypodium distachyon.
29039004	3	25	theme	pathway	611:617	arg1	characterization					567:582	functional characterization	556:582	functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	556:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	3	26	theme	composition	470:480	arg1	information					496:506	xyloglucan composition and structure information	459:506	xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon	459:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	3	27	from	characterization	567:582	arg1	distachyon					635:644	Brachypodium distachyon	622:644	Brachypodium distachyon	622:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
29039004	2	28	theme	sensitive	316:324	arg1	technique					275:283	This technique	270:283	This technique	270:283	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	28	theme	sensitive	316:324	arg1	means					340:344	a rapid, sensitive, and accurate means	307:344	a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures	307:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	2	29	theme	cell	360:363	arg1	structures					385:394	cell wall polysaccharide structures	360:394	cell wall polysaccharide structures	360:394	This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures.
29039004	0	30	theme	Xyloglucan	12:21	arg1	Analysis					33:40	Xyloglucan Structure Analysis	12:40	Xyloglucan Structure Analysis in Brachypodium distachyon	12:67	Methods for Xyloglucan Structure Analysis in Brachypodium distachyon.
29039004	3	31	theme	xyloglucan	587:596	arg1	pathway					611:617	xyloglucan biosynthesis pathway	587:617	xyloglucan biosynthesis pathway in Brachypodium distachyon	587:644	Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
30819031	4	0	theme	heat	966:969	arg1	treatment					971:979	heat treatment	966:979	heat treatment	966:979	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	8	1	theme	new	1710:1712	arg1	solution					1726:1733	a new sustainable solution	1708:1733	a new sustainable solution in the field of consumer products	1708:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	8	1	theme	new	1710:1712	arg1	range					1684:1688	its range	1680:1688	its range of application	1680:1703	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	1	2	theme	mechanical	260:269	arg1	terms					251:255	terms	251:255	terms of mechanical, thermal, and degradation properties	251:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	4	3	theme	electron	801:808	arg1	microscopy					810:819	scanning electron microscopy	792:819	scanning electron microscopy	792:819	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	3	4	theme	TSA	672:674	arg1	21/40					681:685	TSA FSCM 21/40	672:685	TSA FSCM 21/40	672:685	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	4	theme	TSA	672:674	arg1	extruder					662:669	a co-rotating twin-screw extruder	637:669	a co-rotating twin-screw extruder (TSA FSCM 21/40)	637:686	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	5	5	theme	close	1132:1136	arg1	correlation					1138:1148	a close correlation	1130:1148	a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties	1089:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	1	6	with	material	110:117	arg1	properties					134:143	modulable properties	124:143	modulable properties	124:143	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	5	7	from	content	1162:1168	arg1	thermal					1182:1188	thermal	1182:1188	thermal	1182:1188	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	5	7	from	content	1162:1168	arg1	tensile					1173:1179	tensile	1173:1179	tensile	1173:1179	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	5	7	from	content	1162:1168	arg1	properties					1207:1216	degradation properties	1195:1216	degradation properties	1195:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	3	8	theme	content	569:575	arg1	ratio					556:560	ratio	556:560	ratio of MCC content (from 0% to 55% w/w)	556:596	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	1	9	theme	treatments	202:211	arg1	increase					167:174	the increase	163:174	the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties	163:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	6	10	theme	results	1242:1248	arg1	part					1230:1233	The second part	1219:1233	The second part of the results	1219:1248	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	4	11	theme	thermal	1028:1034	arg1	properties					1036:1045	thermal properties	1028:1045	thermal properties	1028:1045	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	8	12	theme	composite	1625:1633	arg1	properties					1599:1608	the properties	1595:1608	the properties of the PVA/MCC composite	1595:1633	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	8	13	with	modular	1646:1652	arg1	aim					1663:1665	the aim	1659:1665	the aim of extending its range of application as a new sustainable solution in the field of consumer products	1659:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	6	14	theme	second	1223:1228	arg1	part					1230:1233	The second part	1219:1233	The second part of the results	1219:1248	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	7	15	theme	mechanical	1484:1493	arg1	parameters					1495:1504	the considered mechanical parameters	1469:1504	the considered mechanical parameters	1469:1504	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	4	16	theme	mechanical	830:839	arg1	tests					841:845	tensile mechanical tests	822:845	tensile mechanical tests	822:845	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	1	17	from	composite	238:246	arg1	terms					251:255	terms	251:255	terms of mechanical, thermal, and degradation properties	251:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	3	18	theme	w/w	593:595	arg1	%					591:591	0% to 55% w/w	583:595	0% to 55% w/w	583:595	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	19	theme	55	589:590	arg1	%					584:584	%	584:584	%	584:584	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	6	20	theme	effects	1279:1285	arg1	analysis					1263:1270	the analysis	1259:1270	the analysis of the effects that the thermal treatments (T1, T2) had on the composite	1259:1343	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	4	21	theme	degradation	886:896	arg1	tests					898:902	water degradation tests	880:902	water degradation tests	880:902	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	2	22	theme	water-soluble	402:414	arg1	PVA					451:453	PVA	451:453	PVA	451:453	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	2	22	theme	water-soluble	402:414	arg1	alcohol					442:448	water-soluble, biodegradable, polyvinyl alcohol	402:448	water-soluble, biodegradable, polyvinyl alcohol (PVA)	402:454	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	1	23	theme	thermal	272:278	arg1	terms					251:255	terms	251:255	terms of mechanical, thermal, and degradation properties	251:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	8	24	theme	application	1693:1703	arg1	solution					1726:1733	a new sustainable solution	1708:1733	a new sustainable solution in the field of consumer products	1708:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	8	24	theme	application	1693:1703	arg1	range					1684:1688	its range	1680:1688	its range of application	1680:1703	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	1	25	theme	PVA/cellulose	80:92	arg1	material					110:117	PVA/cellulose fiber composite material	80:117	PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties	80:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	4	26	theme	MCC	951:953	arg1	ratios					955:960	MCC ratios	951:960	MCC ratios	951:960	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	0	27	theme	Modulable	0:8	arg1	properties					10:19	Modulable properties	0:19	Modulable properties of PVA/cellulose fiber composites	0:53	Modulable properties of PVA/cellulose fiber composites.
30819031	5	28	theme	good	1091:1094	arg1	behavior					1110:1117	a good stress-strain behavior	1089:1117	a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties	1089:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	1	29	theme	composite	100:108	arg1	material					110:117	PVA/cellulose fiber composite material	80:117	PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties	80:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	7	30	theme	PVA	1544:1546	arg1	matrix					1548:1553	the PVA matrix	1540:1553	the PVA matrix	1540:1553	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	8	31	from	solution	1726:1733	arg1	field					1742:1746	the field	1738:1746	the field of consumer products	1738:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	2	32	theme	polyvinyl	432:440	arg1	PVA					451:453	PVA	451:453	PVA	451:453	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	2	32	theme	polyvinyl	432:440	arg1	alcohol					442:448	water-soluble, biodegradable, polyvinyl alcohol	402:448	water-soluble, biodegradable, polyvinyl alcohol (PVA)	402:454	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	1	33	theme	degradation	285:295	arg1	properties					297:306	degradation properties	285:306	degradation properties	285:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	6	34	dep	treatments	1304:1313	arg1	T1					1316:1317	T1	1316:1317	T1	1316:1317	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	6	34	dep	treatments	1304:1313	arg1	T2					1320:1321	T2	1320:1321	T2	1320:1321	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	7	35	theme	water	1413:1417	arg1	stability					1419:1427	the thermal and water stability	1397:1427	stability	1419:1427	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	0	36	theme	fiber	38:42	arg1	composites					44:53	PVA/cellulose fiber composites	24:53	PVA/cellulose fiber composites	24:53	Modulable properties of PVA/cellulose fiber composites.
30819031	4	37	from	influence	938:946	arg1	mechanical					999:1008	mechanical	999:1008	mechanical	999:1008	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	4	37	from	influence	938:946	arg1	morphological					984:996	morphological	984:996	morphological	984:996	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	4	37	from	influence	938:946	arg1	properties					1036:1045	thermal properties	1028:1045	thermal properties	1028:1045	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	4	37	from	influence	938:946	arg1	degradation					1011:1021	degradation	1011:1021	degradation	1011:1021	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	7	38	theme	thermal	1401:1407	arg1	stability					1419:1427	the thermal and water stability	1397:1427	stability	1419:1427	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	2	39	theme	microcrystalline	460:475	arg1	MCC					488:490	MCC	488:490	MCC	488:490	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	2	39	theme	microcrystalline	460:475	arg1	cellulose					477:485	microcrystalline cellulose	460:485	microcrystalline cellulose (MCC)	460:491	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	5	40	theme	PVA/MCC	1061:1067	arg1	composite					1069:1077	The PVA/MCC composite	1057:1077	The PVA/MCC composite	1057:1077	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	7	41	from	improvement	1454:1464	arg1	parameters					1495:1504	the considered mechanical parameters	1469:1504	the considered mechanical parameters	1469:1504	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	6	42	contain	had	1324:1326	arg1	treatments					1304:1313	the thermal treatments	1292:1313	the thermal treatments (T1, T2)	1292:1322	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	6	42	contain	had	1324:1326	arg2	effects					1279:1285	the effects	1275:1285	the effects that the thermal treatments (T1, T2) had on the composite	1275:1343	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	4	43	theme	different	733:741	arg1	conditions					751:760	two different thermal conditions	729:760	two different thermal conditions (T1, T2)	729:769	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	4	43	theme	different	733:741	arg1	T1					763:764	T1	763:764	T1	763:764	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	2	44	theme	experimental	368:379	arg1	formulation					381:391	an experimental formulation	365:391	an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC)	365:491	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	5	45	theme	MCC	1158:1160	arg1	content					1162:1168	MCC content	1158:1168	MCC content on tensile, thermal, and degradation properties	1158:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	7	46	theme	thermal	1355:1361	arg1	treatments					1363:1372	thermal treatments	1355:1372	thermal treatments	1355:1372	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	4	47	theme	treatment	971:979	arg1	influence					938:946	the influence	934:946	the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties	934:1045	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	3	48	theme	twin-screw	651:660	arg1	21/40					681:685	TSA FSCM 21/40	672:685	TSA FSCM 21/40	672:685	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	48	theme	twin-screw	651:660	arg1	extruder					662:669	a co-rotating twin-screw extruder	637:669	a co-rotating twin-screw extruder (TSA FSCM 21/40)	637:686	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	8	49	theme	sustainable	1714:1724	arg1	solution					1726:1733	a new sustainable solution	1708:1733	a new sustainable solution in the field of consumer products	1708:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	8	49	theme	sustainable	1714:1724	arg1	range					1684:1688	its range	1680:1688	its range of application	1680:1703	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	4	50	theme	scanning	792:799	arg1	microscopy					810:819	scanning electron microscopy	792:819	scanning electron microscopy	792:819	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	3	51	theme	FSCM	676:679	arg1	21/40					681:685	TSA FSCM 21/40	672:685	TSA FSCM 21/40	672:685	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	51	theme	FSCM	676:679	arg1	extruder					662:669	a co-rotating twin-screw extruder	637:669	a co-rotating twin-screw extruder (TSA FSCM 21/40)	637:686	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	8	52	theme	present	1572:1578	arg1	work					1580:1583	The present work	1568:1583	The present work	1568:1583	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	1	53	theme	reinforcement	179:191	arg1	increase					167:174	the increase	163:174	the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties	163:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	7	54	from	stability	1419:1427	arg1	parameters					1495:1504	the considered mechanical parameters	1469:1504	the considered mechanical parameters	1469:1504	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	8	55	theme	consumer	1751:1758	arg1	products					1760:1767	consumer products	1751:1767	consumer products	1751:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	3	56	theme	MCC	565:567	arg1	content					569:575	MCC content	565:575	MCC content (from 0% to 55% w/w)	565:596	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	1	57	theme	heat	197:200	arg1	treatments					202:211	heat treatments	197:211	heat treatments	197:211	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	7	58	theme	possible	1515:1522	arg1	crosslinking					1524:1535	a possible crosslinking	1513:1535	a possible crosslinking of the PVA matrix	1513:1553	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	4	59	dep	T1	763:764	arg1	T2					767:768	T2	767:768	T2	767:768	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	1	60	dep	PURPOSE	56:62	arg1	Development					65:75	Development	65:75	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.	56:307	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	2	61	dep	water-soluble	402:414	arg1	biodegradable					417:429	biodegradable	417:429	biodegradable	417:429	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	3	62	theme	0	583:583	arg1	%					584:584	%	584:584	%	584:584	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	63	dep	%	584:584	arg1	to					586:587	to	586:587	to	586:587	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	4	64	theme	thermogravimetric	848:864	arg1	analysis					866:873	thermogravimetric analysis	848:873	thermogravimetric analysis	848:873	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	8	65	theme	PVA/MCC	1617:1623	arg1	composite					1625:1633	the PVA/MCC composite	1613:1633	the PVA/MCC composite	1613:1633	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	1	66	theme	modulable	124:132	arg1	properties					134:143	modulable properties	124:143	modulable properties	124:143	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	7	67	theme	considered	1473:1482	arg1	parameters					1495:1504	the considered mechanical parameters	1469:1504	the considered mechanical parameters	1469:1504	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	3	68	from	ratio	556:560	arg1	%					591:591	0% to 55% w/w	583:595	0% to 55% w/w	583:595	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	4	69	theme	tensile	822:828	arg1	tests					841:845	tensile mechanical tests	822:845	tensile mechanical tests	822:845	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	5	70	theme	degradation	1195:1205	arg1	properties					1207:1216	degradation properties	1195:1216	degradation properties	1195:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	6	71	theme	thermal	1296:1302	arg1	treatments					1304:1313	the thermal treatments	1292:1313	the thermal treatments (T1, T2)	1292:1322	The second part of the results includes the analysis of the effects that the thermal treatments (T1, T2) had on the composite.
30819031	7	72	theme	significant	1442:1452	arg1	improvement					1454:1464	a significant improvement	1440:1464	the thermal and water stability as well as a significant improvement in the considered mechanical parameters	1397:1504	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	8	73	theme	products	1760:1767	arg1	field					1742:1746	the field	1738:1746	the field of consumer products	1738:1767	CONCLUSION: The present work shows how the properties of the PVA/MCC composite can become modular with the aim of extending its range of application as a new sustainable solution in the field of consumer products.
30819031	4	74	theme	water	880:884	arg1	tests					898:902	water degradation tests	880:902	water degradation tests	880:902	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	3	75	from	%	591:591	arg1	ratio					556:560	ratio	556:560	ratio of MCC content (from 0% to 55% w/w)	556:596	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	75	from	%	591:591	arg1	content					569:575	MCC content	565:575	MCC content (from 0% to 55% w/w)	565:596	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	1	76	theme	fiber	94:98	arg1	material					110:117	PVA/cellulose fiber composite material	80:117	PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties	80:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	0	77	theme	PVA/cellulose	24:36	arg1	composites					44:53	PVA/cellulose fiber composites	24:53	PVA/cellulose fiber composites	24:53	Modulable properties of PVA/cellulose fiber composites.
30819031	5	78	theme	stress-strain	1096:1108	arg1	behavior					1110:1117	a good stress-strain behavior	1089:1117	a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties	1089:1216	RESULTS: The PVA/MCC composite exhibited a good stress-strain behavior as well as a close correlation between MCC content on tensile, thermal, and degradation properties.
30819031	1	79	theme	material	110:117	arg1	Development					65:75	Development	65:75	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.	56:307	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	7	80	theme	matrix	1548:1553	arg1	crosslinking					1524:1535	a possible crosslinking	1513:1535	a possible crosslinking of the PVA matrix	1513:1553	In fact, thermal treatments have allowed improving the thermal and water stability as well as a significant improvement in the considered mechanical parameters due to a possible crosslinking of the PVA matrix.
30819031	4	81	theme	ratios	955:960	arg1	influence					938:946	the influence	934:946	the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties	934:1045	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	0	82	theme	composites	44:53	arg1	properties					10:19	Modulable properties	0:19	Modulable properties of PVA/cellulose fiber composites	0:53	Modulable properties of PVA/cellulose fiber composites.
30819031	1	83	theme	properties	297:306	arg1	terms					251:255	terms	251:255	terms of mechanical, thermal, and degradation properties	251:306	PURPOSE: Development of PVA/cellulose fiber composite material with modulable properties, obtained through the increase of reinforcement and heat treatments in order to optimize the composite in terms of mechanical, thermal, and degradation properties.
30819031	4	84	theme	thermal	743:749	arg1	conditions					751:760	two different thermal conditions	729:760	two different thermal conditions (T1, T2)	729:769	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	4	84	theme	thermal	743:749	arg1	T1					763:764	T1	763:764	T1	763:764	Then, samples have been treated through two different thermal conditions (T1, T2) and characterized by scanning electron microscopy, tensile mechanical tests, thermogravimetric analysis, and water degradation tests to investigate, respectively, the influence of MCC ratios and heat treatment on morphological, mechanical, degradation, and thermal properties.
30819031	2	85	dep	formulation	381:391	arg1	matrix					358:363	matrix	358:363	matrix	358:363	METHODS: The composite was designed selecting as matrix an experimental formulation based on water-soluble, biodegradable, polyvinyl alcohol (PVA) and microcrystalline cellulose (MCC), as reinforcement.
30819031	3	86	theme	co-rotating	639:649	arg1	21/40					681:685	TSA FSCM 21/40	672:685	TSA FSCM 21/40	672:685	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	86	theme	co-rotating	639:649	arg1	extruder					662:669	a co-rotating twin-screw extruder	637:669	a co-rotating twin-screw extruder (TSA FSCM 21/40)	637:686	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
30819031	3	87	theme	different	516:524	arg1	formulations					526:537	Six different formulations	512:537	Six different formulations	512:537	Six different formulations, with increasing ratio of MCC content (from 0% to 55% w/w) in PVA, were developed and extruded by a co-rotating twin-screw extruder (TSA FSCM 21/40).
29481951	1	0	theme	efficient	137:145	arg1	degradation					102:112	Soil burial degradation	90:112	Soil burial degradation	90:112	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	0	theme	efficient	137:145	arg1	method					162:167	an efficient waste disposal method	134:167	an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite	134:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	6	1	theme	burial	828:833	arg1	degradation					835:845	the soil burial degradation	819:845	the soil burial degradation	819:845	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	1	2	theme	waste	147:151	arg1	degradation					102:112	Soil burial degradation	90:112	Soil burial degradation	90:112	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	2	theme	waste	147:151	arg1	method					162:167	an efficient waste disposal method	134:167	an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite	134:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	3	theme	starch/poly	234:244	arg1	composite					266:274	starch/poly (lactic acid) (PLA) composite	234:274	starch/poly (lactic acid) (PLA) composite	234:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	8	4	theme	service	1280:1286	arg1	products					1293:1300	short service life products	1274:1300	short service life products	1274:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	6	5	theme	soil	823:826	arg1	degradation					835:845	the soil burial degradation	819:845	the soil burial degradation	819:845	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	4	6	from	rings	703:707	arg1	starch					712:717	starch	712:717	starch	712:717	XPS demonstrated the cleavage of CO linkages between glucose rings in starch.
29481951	4	7	theme	glucose	695:701	arg1	rings					703:707	glucose rings	695:707	glucose rings in starch	695:717	XPS demonstrated the cleavage of CO linkages between glucose rings in starch.
29481951	4	8	theme	CO	675:676	arg1	linkages					678:685	CO linkages	675:685	CO linkages between glucose rings in starch	675:717	XPS demonstrated the cleavage of CO linkages between glucose rings in starch.
29481951	7	9	theme	same	1103:1106	arg1	time					1108:1111	the same time	1099:1111	the same time	1099:1111	The thermal stability of starch/PLA composite shifted towards to that of PLA when they were subjected to soil burial for the same time.
29481951	6	10	contain	had	946:948	arg2	variation					959:967	a slight variation	950:967	a slight variation for PLA	950:975	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	6	10	contain	had	946:948	arg1	it					943:944	it	943:944	it	943:944	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	2	11	theme	thermal	324:330	arg1	properties					332:341	chemical and thermal properties	311:341	chemical and thermal properties of starch/PLA composite	311:365	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	1	12	theme	lactic	247:252	arg1	starch/poly					234:244	starch/poly	234:244	starch/poly (lactic acid) (PLA) composite	234:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	12	theme	lactic	247:252	arg1	acid					254:257	lactic acid	247:257	lactic acid	247:257	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	5	13	theme	starch/PLA	756:765	arg1	composite					767:775	the starch/PLA composite	752:775	the starch/PLA composite	752:775	DSC and TGA results showed that the starch/PLA composite degraded faster than the pure PLA.
29481951	1	14	theme	disposal	153:160	arg1	degradation					102:112	Soil burial degradation	90:112	Soil burial degradation	90:112	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	14	theme	disposal	153:160	arg1	method					162:167	an efficient waste disposal method	134:167	an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite	134:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	2	15	theme	scanning	469:476	arg1	calorimetry					478:488	differential scanning calorimetry	456:488	differential scanning calorimetry (DSC)	456:494	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	15	theme	scanning	469:476	arg1	DSC					491:493	DSC	491:493	DSC	491:493	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	8	16	theme	materials	1255:1263	arg1	markets					1226:1232	the markets	1222:1232	the markets of biodegradable PLA materials used for short service life products	1222:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	5	17	theme	TGA	728:730	arg1	results					732:738	DSC and TGA results	720:738	DSC and TGA results	720:738	DSC and TGA results showed that the starch/PLA composite degraded faster than the pure PLA.
29481951	7	18	theme	starch/PLA	1003:1012	arg1	composite					1014:1022	starch/PLA composite	1003:1022	starch/PLA composite	1003:1022	The thermal stability of starch/PLA composite shifted towards to that of PLA when they were subjected to soil burial for the same time.
29481951	2	19	theme	differential	456:467	arg1	calorimetry					478:488	differential scanning calorimetry	456:488	differential scanning calorimetry (DSC)	456:494	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	19	theme	differential	456:467	arg1	DSC					491:493	DSC	491:493	DSC	491:493	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	8	20	theme	short	1274:1278	arg1	products					1293:1300	short service life products	1274:1300	short service life products	1274:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	2	21	theme	composite	357:365	arg1	properties					332:341	chemical and thermal properties	311:341	chemical and thermal properties of starch/PLA composite	311:365	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	7	22	theme	composite	1014:1022	arg1	stability					990:998	The thermal stability	978:998	The thermal stability of starch/PLA composite	978:1022	The thermal stability of starch/PLA composite shifted towards to that of PLA when they were subjected to soil burial for the same time.
29481951	2	23	theme	starch/PLA	346:355	arg1	composite					357:365	starch/PLA composite	346:365	starch/PLA composite	346:365	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	6	24	theme	composite	895:903	arg1	temperature					869:879	the glass transition temperature	848:879	the glass transition temperature of starch/PLA composite	848:903	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	2	25	theme	thermal	501:507	arg1	analysis					521:528	thermal gravimetric analysis	501:528	thermal gravimetric analysis (TGA)	501:534	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	25	theme	thermal	501:507	arg1	TGA					531:533	TGA	531:533	TGA	531:533	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	1	26	theme	Soil	90:93	arg1	degradation					102:112	Soil burial degradation	90:112	Soil burial degradation	90:112	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	26	theme	Soil	90:93	arg1	method					162:167	an efficient waste disposal method	134:167	an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite	134:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	3	27	theme	XPS	537:539	arg1	results					549:555	XPS and IRM results	537:555	XPS and IRM results	537:555	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	2	28	theme	photoelectron	395:407	arg1	XPS					423:425	XPS	423:425	XPS	423:425	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	28	theme	photoelectron	395:407	arg1	spectroscopy					409:420	X-ray photoelectron spectroscopy	389:420	X-ray photoelectron spectroscopy (XPS)	389:426	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	0	29	theme	chemical	20:27	arg1	changes					41:47	chemical and thermal changes	20:47	chemical and thermal changes	20:47	Soil burial-induced chemical and thermal changes in starch/poly (lactic acid) composites.
29481951	6	30	theme	starch/PLA	884:893	arg1	composite					895:903	starch/PLA composite	884:903	starch/PLA composite	884:903	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	1	31	theme	burial	95:100	arg1	degradation					102:112	Soil burial degradation	90:112	Soil burial degradation	90:112	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	31	theme	burial	95:100	arg1	method					162:167	an efficient waste disposal method	134:167	an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite	134:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	2	32	theme	X-ray	389:393	arg1	XPS					423:425	XPS	423:425	XPS	423:425	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	32	theme	X-ray	389:393	arg1	spectroscopy					409:420	X-ray photoelectron spectroscopy	389:420	X-ray photoelectron spectroscopy (XPS)	389:426	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	3	33	from	groups	620:625	arg1	chains					634:639	PLA chains	630:639	PLA chains	630:639	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	3	34	theme	PLA	594:596	arg1	biodegradation					576:589	the biodegradation	572:589	the biodegradation of PLA	572:596	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	0	35	dep	starch/poly	52:62	arg1	acid					72:75	lactic acid	65:75	lactic acid	65:75	Soil burial-induced chemical and thermal changes in starch/poly (lactic acid) composites.
29481951	5	36	theme	pure	802:805	arg1	PLA					807:809	the pure PLA	798:809	the pure PLA	798:809	DSC and TGA results showed that the starch/PLA composite degraded faster than the pure PLA.
29481951	8	37	theme	PLA-based	1182:1190	arg1	materials					1192:1200	PLA-based materials	1182:1200	PLA-based materials	1182:1200	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	0	38	theme	thermal	33:39	arg1	changes					41:47	chemical and thermal changes	20:47	chemical and thermal changes	20:47	Soil burial-induced chemical and thermal changes in starch/poly (lactic acid) composites.
29481951	1	39	with	materials	191:199	arg1	life					220:223	short service life	206:223	short service life	206:223	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	40	theme	short	206:210	arg1	life					220:223	short service life	206:223	short service life	206:223	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	0	41	theme	starch/poly	52:62	arg1	composites					78:87	starch/poly (lactic acid) composites	52:87	starch/poly (lactic acid) composites	52:87	Soil burial-induced chemical and thermal changes in starch/poly (lactic acid) composites.
29481951	6	42	theme	transition	858:867	arg1	temperature					869:879	the glass transition temperature	848:879	the glass transition temperature of starch/PLA composite	848:903	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	8	43	dep	accelerate	1152:1161	arg1	enlarge					1214:1220	enlarge	1214:1220	will enlarge the markets of biodegradable PLA materials used for short service life products	1209:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	2	44	theme	chemical	311:318	arg1	properties					332:341	chemical and thermal properties	311:341	chemical and thermal properties of starch/PLA composite	311:365	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	7	45	theme	thermal	982:988	arg1	stability					990:998	The thermal stability	978:998	The thermal stability of starch/PLA composite	978:1022	The thermal stability of starch/PLA composite shifted towards to that of PLA when they were subjected to soil burial for the same time.
29481951	6	46	theme	glass	852:856	arg1	temperature					869:879	the glass transition temperature	848:879	the glass transition temperature of starch/PLA composite	848:903	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
29481951	8	47	theme	materials	1192:1200	arg1	degradation					1167:1177	the degradation	1163:1177	the degradation of PLA-based materials	1163:1200	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	3	48	theme	ester	614:618	arg1	groups					620:625	the ester groups	610:625	the ester groups in PLA chains	610:639	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	4	49	theme	linkages	678:685	arg1	cleavage					663:670	the cleavage	659:670	the cleavage of CO linkages between glucose rings in starch	659:717	XPS demonstrated the cleavage of CO linkages between glucose rings in starch.
29481951	1	50	theme	service	212:218	arg1	life					220:223	short service life	206:223	short service life	206:223	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	2	51	theme	gravimetric	509:519	arg1	analysis					521:528	thermal gravimetric analysis	501:528	thermal gravimetric analysis (TGA)	501:534	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	51	theme	gravimetric	509:519	arg1	TGA					531:533	TGA	531:533	TGA	531:533	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	8	52	theme	PLA	1251:1253	arg1	materials					1255:1263	biodegradable PLA materials	1237:1263	biodegradable PLA materials used for short service life products	1237:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	2	53	theme	biodegradation	281:294	arg1	behavior					296:303	The biodegradation behavior	277:303	The biodegradation behavior about chemical and thermal properties of starch/PLA composite	277:365	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	8	54	theme	biodegradable	1237:1249	arg1	materials					1255:1263	biodegradable PLA materials	1237:1263	biodegradable PLA materials used for short service life products	1237:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	0	55	theme	lactic	65:70	arg1	acid					72:75	lactic acid	65:75	lactic acid	65:75	Soil burial-induced chemical and thermal changes in starch/poly (lactic acid) composites.
29481951	8	56	theme	life	1288:1291	arg1	products					1293:1300	short service life products	1274:1300	short service life products	1274:1300	It is established that the starch can accelerate the degradation of PLA-based materials, which will enlarge the markets of biodegradable PLA materials used for short service life products.
29481951	3	57	theme	IRM	545:547	arg1	results					549:555	XPS and IRM results	537:555	XPS and IRM results	537:555	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	1	58	theme	biodegradable	177:189	arg1	materials					191:199	the biodegradable materials	173:199	the biodegradable materials	173:199	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	1	58	theme	biodegradable	177:189	arg1	composite					266:274	starch/poly (lactic acid) (PLA) composite	234:274	starch/poly (lactic acid) (PLA) composite	234:274	Soil burial degradation was confirmed to be an efficient waste disposal method for the biodegradable materials with short service life, such as starch/poly (lactic acid) (PLA) composite.
29481951	5	59	theme	DSC	720:722	arg1	results					732:738	DSC and TGA results	720:738	DSC and TGA results	720:738	DSC and TGA results showed that the starch/PLA composite degraded faster than the pure PLA.
29481951	2	60	theme	infrared	429:436	arg1	IRM					450:452	IRM	450:452	IRM	450:452	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	2	60	theme	infrared	429:436	arg1	microscopy					438:447	infrared microscopy	429:447	infrared microscopy (IRM)	429:453	The biodegradation behavior about chemical and thermal properties of starch/PLA composite was analyzed by using X-ray photoelectron spectroscopy (XPS), infrared microscopy (IRM), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA).
29481951	3	61	theme	PLA	630:632	arg1	chains					634:639	PLA chains	630:639	PLA chains	630:639	XPS and IRM results indicated that the biodegradation of PLA occurred at the ester groups in PLA chains.
29481951	7	62	theme	soil	1083:1086	arg1	burial					1088:1093	soil burial	1083:1093	soil burial	1083:1093	The thermal stability of starch/PLA composite shifted towards to that of PLA when they were subjected to soil burial for the same time.
29481951	6	63	theme	slight	952:957	arg1	variation					959:967	a slight variation	950:967	a slight variation for PLA	950:975	During the soil burial degradation, the glass transition temperature of starch/PLA composite exhibited an obviously decrease while it had a slight variation for PLA.
31419562	0	0	theme	methylene	95:103	arg1	removal					84:90	highly efficient removal	67:90	highly efficient removal of methylene blue and tetracycline from water	67:136	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	8	1	from	application	1249:1259	arg1	removal					1268:1274	the removal	1264:1274	the removal of MB and TC from water	1264:1298	Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.
31419562	2	2	theme	sulfated	391:398	arg1	SCGs					420:423	SCGs	420:423	SCGs	420:423	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	2	2	theme	sulfated	391:398	arg1	aerogels					410:417	sulfated composite aerogels	391:417	sulfated composite aerogels (SCGs)	391:424	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	1	3	theme	freeze-drying	266:278	arg1	process					280:286	a solution mixing-regeneration and freeze-drying process	231:286	process	280:286	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	7	4	theme	original	1125:1132	arg1	integrity					1145:1153	the original structural integrity	1121:1153	the original structural integrity	1121:1153	Furthermore, its adsorption capacity was not apparently deteriorated after seven cycles for MB and ten cycles for TC while the original structural integrity was almost maintained.
31419562	1	5	theme	porous	169:174	arg1	composites					176:185	Cellulose/graphene oxide (CG) porous composites	139:185	Cellulose/graphene oxide (CG) porous composites with 3D network structure	139:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	2	6	theme	reaction	360:367	arg1	conditions					369:378	mild reaction conditions	355:378	mild reaction conditions	355:378	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	0	7	theme	blue	105:108	arg1	methylene					95:103	methylene blue	95:108	methylene blue	95:108	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	6	8	theme	%	995:995	arg1	efficiency					979:988	a removal efficiency	969:988	a removal efficiency of 99%	969:995	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	8	9	from	water	1294:1298	arg1	removal					1268:1274	the removal	1264:1274	the removal of MB and TC from water	1264:1298	Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.
31419562	2	10	theme	mild	355:358	arg1	conditions					369:378	mild reaction conditions	355:378	mild reaction conditions	355:378	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	6	11	contain	containing	913:922	arg1	wastewater					902:911	the simulated wastewater	888:911	the simulated wastewater containing MB and TC	888:932	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	6	11	contain	containing	913:922	arg2	TC					931:932	TC	931:932	TC	931:932	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	6	11	contain	containing	913:922	arg2	MB					924:925	MB	924:925	MB	924:925	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	4	12	theme	maximal	723:729	arg1	capacity					738:745	the maximal uptake capacity	719:745	the maximal uptake capacity	719:745	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	0	13	theme	tetracycline	114:125	arg1	removal					84:90	highly efficient removal	67:90	highly efficient removal of methylene blue and tetracycline from water	67:136	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	4	14	theme	fitting	608:614	arg1	results					616:622	The fitting results	604:622	The fitting results of adsorption models	604:643	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	2	15	theme	CG	293:294	arg1	aerogels					296:303	The CG aerogels	289:303	The CG aerogels	289:303	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	0	16	theme	3D	17:18	arg1	composites					52:61	Sulfo-functional 3D porous cellulose/graphene oxide composites	0:61	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.	0:137	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	3	17	theme	aerogel	548:554	arg1	ability					533:539	The adsorption ability	518:539	The adsorption ability of SCG aerogel	518:554	The adsorption ability of SCG aerogel was greatly enhanced compared with CG aerogels.
31419562	1	18	theme	3D	192:193	arg1	structure					203:211	3D network structure	192:211	3D network structure	192:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	0	19	theme	Sulfo-functional	0:15	arg1	composites					52:61	Sulfo-functional 3D porous cellulose/graphene oxide composites	0:61	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.	0:137	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	4	20	theme	blue	783:786	arg1	MB					789:790	MB	789:790	MB	789:790	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	4	20	theme	blue	783:786	arg1	methylene					773:781	methylene blue	773:786	methylene blue (MB)	773:791	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	1	21	theme	network	195:201	arg1	structure					203:211	3D network structure	192:211	3D network structure	192:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	0	22	theme	cellulose/graphene	27:44	arg1	composites					52:61	Sulfo-functional 3D porous cellulose/graphene oxide composites	0:61	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.	0:137	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	5	23	theme	adsorption	835:844	arg1	mechanism					846:854	The adsorption mechanism	831:854	The adsorption mechanism	831:854	The adsorption mechanism was also studied in detail.
31419562	2	24	theme	1,4-butane	330:339	arg1	sultone					341:347	1,4-butane sultone	330:347	1,4-butane sultone	330:347	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	2	25	theme	sulfo	440:444	arg1	groups					446:451	the sulfo groups	436:451	the sulfo groups	436:451	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	0	26	theme	porous	20:25	arg1	composites					52:61	Sulfo-functional 3D porous cellulose/graphene oxide composites	0:61	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.	0:137	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	6	27	theme	simulated	892:900	arg1	wastewater					902:911	the simulated wastewater	888:911	the simulated wastewater containing MB and TC	888:932	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	3	28	theme	CG	591:592	arg1	aerogels					594:601	CG aerogels	591:601	CG aerogels	591:601	The adsorption ability of SCG aerogel was greatly enhanced compared with CG aerogels.
31419562	4	29	theme	monolayer	659:667	arg1	adsorption					669:678	the monolayer adsorption	655:678	the monolayer adsorption	655:678	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	2	30	theme	cellulose	496:504	arg1	components					506:515	cellulose components	496:515	cellulose components	496:515	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	6	31	theme	removal	971:977	arg1	efficiency					979:988	a removal efficiency	969:988	a removal efficiency of 99%	969:995	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	8	32	theme	TC	1286:1287	arg1	removal					1268:1274	the removal	1264:1274	the removal of MB and TC from water	1264:1298	Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.
31419562	4	33	theme	chemisorptive	684:696	arg1	characteristics					698:712	chemisorptive characteristics	684:712	chemisorptive characteristics	684:712	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	0	34	theme	oxide	46:50	arg1	composites					52:61	Sulfo-functional 3D porous cellulose/graphene oxide composites	0:61	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.	0:137	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	7	35	theme	structural	1134:1143	arg1	integrity					1145:1153	the original structural integrity	1121:1153	the original structural integrity	1121:1153	Furthermore, its adsorption capacity was not apparently deteriorated after seven cycles for MB and ten cycles for TC while the original structural integrity was almost maintained.
31419562	4	36	theme	adsorption	627:636	arg1	models					638:643	adsorption models	627:643	adsorption models	627:643	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	6	37	theme	adsorbent	949:957	arg1	SCG					945:947	the novel SCG adsorbent	935:957	the novel SCG adsorbent	935:957	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	3	38	theme	adsorption	522:531	arg1	ability					533:539	The adsorption ability	518:539	The adsorption ability of SCG aerogel	518:554	The adsorption ability of SCG aerogel was greatly enhanced compared with CG aerogels.
31419562	1	39	with	composites	176:185	arg1	structure					203:211	3D network structure	192:211	3D network structure	192:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	4	40	theme	uptake	731:736	arg1	capacity					738:745	the maximal uptake capacity	719:745	the maximal uptake capacity	719:745	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	1	41	theme	Cellulose/graphene	139:156	arg1	CG					165:166	CG	165:166	CG	165:166	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	1	41	theme	Cellulose/graphene	139:156	arg1	oxide					158:162	Cellulose/graphene oxide	139:162	Cellulose/graphene oxide (CG) porous composites with 3D network structure	139:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	4	42	theme	models	638:643	arg1	results					616:622	The fitting results	604:622	The fitting results of adsorption models	604:643	The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC).
31419562	7	43	theme	adsorption	1015:1024	arg1	capacity					1026:1033	its adsorption capacity	1011:1033	its adsorption capacity	1011:1033	Furthermore, its adsorption capacity was not apparently deteriorated after seven cycles for MB and ten cycles for TC while the original structural integrity was almost maintained.
31419562	1	44	theme	oxide	158:162	arg1	composites					176:185	Cellulose/graphene oxide (CG) porous composites	139:185	Cellulose/graphene oxide (CG) porous composites with 3D network structure	139:211	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	8	45	theme	potential	1239:1247	arg1	application					1249:1259	the potential application	1235:1259	the potential application on the removal of MB and TC from water	1235:1298	Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.
31419562	1	46	theme	solution	233:240	arg1	mixing-regeneration					242:260	a solution mixing-regeneration and freeze-drying process	231:286	mixing-regeneration	242:260	Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process.
31419562	8	47	theme	MB	1279:1280	arg1	removal					1268:1274	the removal	1264:1274	the removal of MB and TC from water	1264:1298	Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.
31419562	3	48	theme	SCG	544:546	arg1	aerogel					548:554	SCG aerogel	544:554	SCG aerogel	544:554	The adsorption ability of SCG aerogel was greatly enhanced compared with CG aerogels.
31419562	0	49	theme	efficient	74:82	arg1	removal					84:90	highly efficient removal	67:90	highly efficient removal of methylene blue and tetracycline from water	67:136	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
31419562	6	50	theme	novel	939:943	arg1	SCG					945:947	the novel SCG adsorbent	935:957	the novel SCG adsorbent	935:957	For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%.
31419562	2	51	theme	composite	400:408	arg1	SCGs					420:423	SCGs	420:423	SCGs	420:423	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	2	51	theme	composite	400:408	arg1	aerogels					410:417	sulfated composite aerogels	391:417	sulfated composite aerogels (SCGs)	391:424	The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components.
31419562	0	52	from	water	132:136	arg1	removal					84:90	highly efficient removal	67:90	highly efficient removal of methylene blue and tetracycline from water	67:136	Sulfo-functional 3D porous cellulose/graphene oxide composites for highly efficient removal of methylene blue and tetracycline from water.
29853074	5	0	theme	osteogenic	827:836	arg1	properties					838:847	osteogenic properties	827:847	osteogenic properties	827:847	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	2	1	theme	phosphate/chitosan	352:369	arg1	composite					380:388	The calcium phosphate/chitosan (CaP/CS) composite	340:388	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF	340:406	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	6	2	from	adhesion	979:986	arg1	composites					1033:1042	nanostructured CaP/CS composites	1011:1042	nanostructured CaP/CS composites	1011:1042	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	6	3	theme	cell	974:977	arg1	adhesion					979:986	improved cell adhesion	965:986	improved cell adhesion	965:986	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	2	4	theme	calcium	344:350	arg1	composite					380:388	The calcium phosphate/chitosan (CaP/CS) composite	340:388	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF	340:406	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	7	5	theme	bone	1130:1133	arg1	engineering					1142:1152	bone tissue engineering	1130:1152	bone tissue engineering	1130:1152	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	6	6	theme	improved	965:972	arg1	adhesion					979:986	improved cell adhesion	965:986	improved cell adhesion	965:986	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	3	7	theme	composites	563:572	arg1	post-treatment					538:551	NaOH post-treatment	533:551	NaOH post-treatment	533:551	The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation.
29853074	1	8	theme	titanium	265:272	arg1	substrate					274:282	the titanium substrate	261:282	the titanium substrate	261:282	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	0	9	theme	modified	101:108	arg1	SBF					110:112	modified SBF	101:112	modified SBF	101:112	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	5	10	theme	composite	874:882	arg1	adhesion					814:821	Cell adhesion	809:821	Cell adhesion	809:821	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	5	10	theme	composite	874:882	arg1	properties					838:847	osteogenic properties	827:847	osteogenic properties	827:847	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	1	11	theme	chitosan	176:183	arg1	bioactivity					161:171	bioactivity	161:171	bioactivity	161:171	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	1	11	theme	chitosan	176:183	arg1	biocompatibility					140:155	biocompatibility	140:155	biocompatibility	140:155	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	5	12	theme	Cell	809:812	arg1	adhesion					814:821	Cell adhesion	809:821	Cell adhesion	809:821	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	3	13	theme	CaP/CS	556:561	arg1	composites					563:572	CaP/CS composites	556:572	CaP/CS composites	556:572	The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation.
29853074	2	14	theme	nano-clusters	455:467	arg1	distribution					429:440	a homogeneous distribution	415:440	a homogeneous distribution of spherical nano-clusters	415:467	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	6	15	theme	in	943:944	arg1	study					952:956	This in vitro study	938:956	This in vitro study	938:956	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	6	16	from	differentiation	992:1006	arg1	composites					1033:1042	nanostructured CaP/CS composites	1011:1042	nanostructured CaP/CS composites	1011:1042	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	4	17	theme	osteoblast-like	708:722	arg1	cells					730:734	human osteoblast-like MG-63 cells	702:734	human osteoblast-like MG-63 cells	702:734	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	2	18	theme	spherical	445:453	arg1	nano-clusters					455:467	spherical nano-clusters	445:467	spherical nano-clusters	445:467	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	3	19	theme	apatite	595:601	arg1	formation					603:611	apatite formation	595:611	apatite formation	595:611	The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation.
29853074	4	20	theme	human	702:706	arg1	cells					730:734	human osteoblast-like MG-63 cells	702:734	human osteoblast-like MG-63 cells	702:734	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	5	21	theme	ALPase	910:915	arg1	assay					917:921	ALPase assay	910:921	ALPase assay	910:921	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	4	22	theme	bromide	794:800	arg1	assay					802:806	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay	741:806	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay	741:806	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	1	23	dep	biocompatibility	140:155	arg1	the					136:138	the	136:138	the	136:138	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	2	24	theme	homogeneous	417:427	arg1	distribution					429:440	a homogeneous distribution	415:440	a homogeneous distribution of spherical nano-clusters	415:467	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	0	25	theme	biological	16:25	arg1	properties					27:36	biological properties	16:36	biological properties	16:36	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	2	26	theme	CaP/CS	372:377	arg1	composite					380:388	The calcium phosphate/chitosan (CaP/CS) composite	340:388	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF	340:406	The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters.
29853074	0	27	theme	calcium	41:47	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	0	27	theme	calcium	41:47	arg1	properties					27:36	biological properties	16:36	biological properties	16:36	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	1	28	theme	modified	301:308	arg1	m-SBF					332:336	m-SBF	332:336	m-SBF	332:336	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	1	28	theme	modified	301:308	arg1	fluid					325:329	modified simulated body fluid	301:329	modified simulated body fluid (m-SBF)	301:337	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	7	29	theme	tissue	1135:1140	arg1	engineering					1142:1152	bone tissue engineering	1130:1152	bone tissue engineering	1130:1152	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	0	30	from	titanium	89:96	arg1	SBF					110:112	modified SBF	101:112	modified SBF	101:112	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	6	31	dep	in	943:944	arg1	vitro					946:950	vitro	946:950	vitro	946:950	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	4	32	theme	cellular	681:688	arg1	response					690:697	the cellular response	677:697	the cellular response of human osteoblast-like MG-63 cells	677:734	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	4	33	theme	cells	730:734	arg1	response					690:697	the cellular response	677:697	the cellular response of human osteoblast-like MG-63 cells	677:734	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	1	34	theme	simulated	310:318	arg1	m-SBF					332:336	m-SBF	332:336	m-SBF	332:336	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	1	34	theme	simulated	310:318	arg1	fluid					325:329	modified simulated body fluid	301:329	modified simulated body fluid (m-SBF)	301:337	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	0	35	theme	composite	68:76	arg1	coating					78:84	composite coating	68:84	composite coating	68:84	Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
29853074	1	36	theme	body	320:323	arg1	m-SBF					332:336	m-SBF	332:336	m-SBF	332:336	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	1	36	theme	body	320:323	arg1	fluid					325:329	modified simulated body fluid	301:329	modified simulated body fluid (m-SBF)	301:337	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	7	37	theme	promising	1106:1114	arg1	candidate					1116:1124	a promising candidate	1104:1124	a promising candidate for bone tissue engineering	1104:1152	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	7	37	theme	promising	1106:1114	arg1	composite					1085:1093	this CaP/CS composite	1073:1093	this CaP/CS composite	1073:1093	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	5	38	theme	CaP/CS	867:872	arg1	composite					874:882	the mesoporous CaP/CS composite	852:882	the mesoporous CaP/CS composite	852:882	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	1	39	from	layer	252:256	arg1	substrate					274:282	the titanium substrate	261:282	the titanium substrate	261:282	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	6	40	theme	CaP/CS	1026:1031	arg1	composites					1033:1042	nanostructured CaP/CS composites	1011:1042	nanostructured CaP/CS composites	1011:1042	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29853074	4	41	theme	-2,5-diphenyltetrazolium	769:792	arg1	assay					802:806	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay	741:806	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay	741:806	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	4	42	theme	coatings	638:645	arg1	Biocompatibility					614:629	Biocompatibility	614:629	Biocompatibility of the coatings	614:645	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	3	43	theme	coatings	496:503	arg1	hydrophilicity					474:487	The hydrophilicity	470:487	The hydrophilicity of the coatings	470:503	The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation.
29853074	1	44	theme	spin-coated	231:241	arg1	layer					252:256	the spin-coated chitosan layer	227:256	the spin-coated chitosan layer on the titanium substrate	227:282	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	7	45	theme	CaP/CS	1078:1083	arg1	candidate					1116:1124	a promising candidate	1104:1124	a promising candidate for bone tissue engineering	1104:1152	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	7	45	theme	CaP/CS	1078:1083	arg1	composite					1085:1093	this CaP/CS composite	1073:1093	this CaP/CS composite	1073:1093	These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
29853074	3	46	theme	NaOH	533:536	arg1	post-treatment					538:551	NaOH post-treatment	533:551	NaOH post-treatment	533:551	The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation.
29853074	1	47	theme	chitosan	243:250	arg1	layer					252:256	the spin-coated chitosan layer	227:256	the spin-coated chitosan layer on the titanium substrate	227:282	In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF).
29853074	5	48	theme	mesoporous	856:865	arg1	composite					874:882	the mesoporous CaP/CS composite	852:882	the mesoporous CaP/CS composite	852:882	Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively.
29853074	4	49	theme	MG-63	724:728	arg1	cells					730:734	human osteoblast-like MG-63 cells	702:734	human osteoblast-like MG-63 cells	702:734	Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
29853074	6	50	theme	nanostructured	1011:1024	arg1	composites					1033:1042	nanostructured CaP/CS composites	1011:1042	nanostructured CaP/CS composites	1011:1042	This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites.
29464600	0	0	theme	composites	70:79	arg1	study					12:16	Comparative study	0:16	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.	0:117	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	1	1	theme	novel	135:139	arg1	biochar					164:170	ball-milled biochar	152:170	ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB)	152:229	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	1	theme	novel	135:139	arg1	alternative					254:264	an alternative	251:264	an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution	251:338	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	1	theme	novel	135:139	arg1	composite					141:149	a novel composite	133:149	a novel composite	133:149	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	7	2	from	solution	1233:1240	arg1	CA-BMB					1144:1149	CA-BMB	1144:1149	CA-BMB	1144:1149	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	7	2	from	solution	1233:1240	arg1	removal					1209:1215	a low-cost and eco-friendly adsorbent for MB removal	1164:1215	a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution	1164:1240	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	0	3	from	study	12:16	arg1	adsorption					107:116	aqueous methylene blue adsorption	84:116	aqueous methylene blue adsorption	84:116	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	6	4	theme	Langmuir	928:935	arg1	models					937:942	the Langmuir models	924:942	the Langmuir models	924:942	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	0	5	theme	aqueous methylene	84:100	arg1	adsorption					107:116	aqueous methylene blue adsorption	84:116	aqueous methylene blue adsorption	84:116	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	1	6	theme	methylene	295:303	arg1	removal					284:290	the removal	280:290	the removal of methylene blue (MB) from an aqueous solution	280:338	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	5	7	theme	surface	831:837	arg1	indicative					783:792	indicative	783:792	indicative	783:792	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	6	8	theme	CA-BMB	894:899	arg1	isotherms					868:876	Adsorption isotherms	857:876	Adsorption isotherms of BMB, CA, and CA-BMB	857:899	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	6	9	theme	Adsorption	857:866	arg1	isotherms					868:876	Adsorption isotherms	857:876	Adsorption isotherms of BMB, CA, and CA-BMB	857:899	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	1	10	from	solution	331:338	arg1	removal					284:290	the removal	280:290	the removal of methylene blue (MB) from an aqueous solution	280:338	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	11	theme	blue	305:308	arg1	methylene					295:303	methylene blue	295:308	methylene blue (MB)	295:313	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	11	theme	blue	305:308	arg1	MB					311:312	MB	311:312	MB	311:312	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	3	12	theme	75	463:464	arg1	%					465:465	%	465:465	%	465:465	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	5	13	theme	indicative	783:792	arg1	model					776:780	the Ritchie's kinetic model	754:780	the Ritchie's kinetic model	754:780	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	4	14	dep	achieved	631:638	arg1	followed					652:659	followed	652:659	followed by slow kinetics reaching 91% removal in 16 h	652:705	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	3	15	theme	%	453:453	arg1	BMB					455:457	25% BMB	451:457	25% BMB	451:457	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	0	16	theme	blue	102:105	arg1	adsorption					107:116	aqueous methylene blue adsorption	84:116	aqueous methylene blue adsorption	84:116	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	5	17	theme	composite	846:854	arg1	surface					831:837	energetically heterogeneous solid surface	797:837	energetically heterogeneous solid surface of the composite	797:854	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	1	18	theme	ball-milled	152:162	arg1	biochar					164:170	ball-milled biochar	152:170	ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB)	152:229	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	18	theme	ball-milled	152:162	arg1	BMB					173:175	BMB	173:175	BMB	173:175	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	18	theme	ball-milled	152:162	arg1	composite					141:149	a novel composite	133:149	a novel composite	133:149	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	0	19	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.	0:117	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	4	20	theme	50 mg L-1	562:570	arg1	concentration					545:557	an initial MB concentration	531:557	an initial MB concentration of 50 mg L-1	531:570	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	0	21	theme	calcium	21:27	arg1	alginate					29:36	calcium alginate	21:36	calcium alginate	21:36	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	3	22	theme	%	465:465	arg1	alginate					467:474	75% alginate	463:474	75% alginate	463:474	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	7	23	theme	adsorbent	1192:1200	arg1	CA-BMB					1144:1149	CA-BMB	1144:1149	CA-BMB	1144:1149	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	7	23	theme	adsorbent	1192:1200	arg1	removal					1209:1215	a low-cost and eco-friendly adsorbent for MB removal	1164:1215	a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution	1164:1240	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	7	24	theme	adsorption	1106:1115	arg1	performance					1117:1127	The outstanding adsorption performance	1090:1127	The outstanding adsorption performance	1090:1127	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	6	25	theme	adsorption	949:958	arg1	capacity					960:967	the adsorption capacity	945:967	the adsorption capacity of CA-BMB (1210.7 mg g-1)	945:993	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	6	25	theme	adsorption	949:958	arg1	that					1008:1011	that	1008:1011	that	1008:1011	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	7	26	theme	aqueous	1225:1231	arg1	solution					1233:1240	an aqueous solution	1222:1240	an aqueous solution	1222:1240	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	5	27	theme	solid	825:829	arg1	surface					831:837	energetically heterogeneous solid surface	797:837	energetically heterogeneous solid surface of the composite	797:854	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	2	28	theme	CA-BMB	394:399	arg1	composite					401:409	CA-BMB composite	394:409	CA-BMB composite	394:409	Sorption performance was compared among CA, BMB, and CA-BMB composite with batch adsorption experiments.
29464600	7	29	theme	outstanding	1094:1104	arg1	performance					1117:1127	The outstanding adsorption performance	1090:1127	The outstanding adsorption performance	1090:1127	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	4	30	theme	MB	542:543	arg1	concentration					545:557	an initial MB concentration	531:557	an initial MB concentration of 50 mg L-1	531:570	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	4	31	theme	%	689:689	arg1	removal					691:697	91% removal	687:697	91% removal	687:697	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	3	32	from	CA	505:506	arg1	adsorption					514:523	MB adsorption	511:523	MB adsorption	511:523	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	2	33	theme	Sorption	341:348	arg1	performance					350:360	Sorption performance	341:360	Sorption performance	341:360	Sorption performance was compared among CA, BMB, and CA-BMB composite with batch adsorption experiments.
29464600	0	34	theme	alginate	29:36	arg1	study					12:16	Comparative study	0:16	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.	0:117	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	1	35	theme	aqueous	323:329	arg1	solution					331:338	an aqueous solution	320:338	an aqueous solution	320:338	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	4	36	theme	91	687:688	arg1	%					689:689	%	689:689	%	689:689	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	4	37	theme	initial	534:540	arg1	concentration					545:557	an initial MB concentration	531:557	an initial MB concentration of 50 mg L-1	531:570	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	0	38	theme	biochar	51:57	arg1	study					12:16	Comparative study	0:16	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.	0:117	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	3	39	theme	new	481:483	arg1	composite					485:493	the new composite	477:493	the new composite	477:493	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	4	40	theme	%	604:604	arg1	removal					609:615	74% MB removal	602:615	74% MB removal by CA-BMB	602:625	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	0	41	theme	ball-milled	39:49	arg1	biochar					51:57	ball-milled biochar	39:57	ball-milled biochar	39:57	Comparative study of calcium alginate, ball-milled biochar, and their composites on aqueous methylene blue adsorption.
29464600	1	42	theme	adsorbent	266:274	arg1	alternative					254:264	an alternative	251:264	an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution	251:338	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	42	theme	adsorbent	266:274	arg1	composite					141:149	a novel composite	133:149	a novel composite	133:149	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	4	43	theme	74	602:603	arg1	%					604:604	%	604:604	%	604:604	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	4	44	theme	slow	664:667	arg1	kinetics					669:676	slow kinetics	664:676	slow kinetics reaching 91% removal in 16 h	664:705	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	4	45	theme	kinetics	573:580	arg1	studies					582:588	kinetics studies	573:588	kinetics studies	573:588	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	5	46	theme	heterogeneous	811:823	arg1	surface					831:837	energetically heterogeneous solid surface	797:837	energetically heterogeneous solid surface of the composite	797:854	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	1	47	theme	calcium-alginate	194:209	arg1	CA-BMB					223:228	CA-BMB	223:228	CA-BMB	223:228	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	47	theme	calcium-alginate	194:209	arg1	beads					216:220	calcium-alginate (CA) beads	194:220	calcium-alginate (CA) beads (CA-BMB)	194:229	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	5	48	theme	kinetic	768:774	arg1	model					776:780	the Ritchie's kinetic model	754:780	the Ritchie's kinetic model	754:780	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	7	49	theme	low-cost	1166:1173	arg1	CA-BMB					1144:1149	CA-BMB	1144:1149	CA-BMB	1144:1149	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	7	49	theme	low-cost	1166:1173	arg1	removal					1209:1215	a low-cost and eco-friendly adsorbent for MB removal	1164:1215	a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution	1164:1240	The outstanding adsorption performance suggested that CA-BMB can serve as a low-cost and eco-friendly adsorbent for MB removal from an aqueous solution.
29464600	4	50	theme	MB	606:607	arg1	removal					609:615	74% MB removal	602:615	74% MB removal by CA-BMB	602:625	With an initial MB concentration of 50 mg L-1, kinetics studies showed that 74% MB removal by CA-BMB was achieved within 8 h, followed by slow kinetics reaching 91% removal in 16 h.
29464600	6	51	theme	CA-BMB	972:977	arg1	capacity					960:967	the adsorption capacity	945:967	the adsorption capacity of CA-BMB (1210.7 mg g-1)	945:993	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	6	51	theme	CA-BMB	972:977	arg1	that					1008:1011	that	1008:1011	that	1008:1011	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	6	52	theme	CA	886:887	arg1	isotherms					868:876	Adsorption isotherms	857:876	Adsorption isotherms of BMB, CA, and CA-BMB	857:899	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
29464600	2	53	theme	adsorption	422:431	arg1	experiments					433:443	batch adsorption experiments	416:443	batch adsorption experiments	416:443	Sorption performance was compared among CA, BMB, and CA-BMB composite with batch adsorption experiments.
29464600	5	54	theme	adsorption	712:721	arg1	kinetics					723:730	The adsorption kinetics	708:730	The adsorption kinetics	708:730	The adsorption kinetics was well explained by the Ritchie's kinetic model, indicative of energetically heterogeneous solid surface of the composite.
29464600	1	55	theme	CA	212:213	arg1	CA-BMB					223:228	CA-BMB	223:228	CA-BMB	223:228	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	1	55	theme	CA	212:213	arg1	beads					216:220	calcium-alginate (CA) beads	194:220	calcium-alginate (CA) beads (CA-BMB)	194:229	In this work, a novel composite, ball-milled biochar (BMB) encapsulated in calcium-alginate (CA) beads (CA-BMB), was synthesized as an alternative adsorbent for the removal of methylene blue (MB) from an aqueous solution.
29464600	2	56	theme	batch	416:420	arg1	experiments					433:443	batch adsorption experiments	416:443	batch adsorption experiments	416:443	Sorption performance was compared among CA, BMB, and CA-BMB composite with batch adsorption experiments.
29464600	3	57	theme	MB	511:512	arg1	adsorption					514:523	MB adsorption	511:523	MB adsorption	511:523	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	3	58	theme	25	451:452	arg1	%					453:453	%	453:453	%	453:453	With 25% BMB and 75% alginate, the new composite resembled CA in MB adsorption.
29464600	6	59	theme	BMB	881:883	arg1	isotherms					868:876	Adsorption isotherms	857:876	Adsorption isotherms of BMB, CA, and CA-BMB	857:899	Adsorption isotherms of BMB, CA, and CA-BMB can all be fitted with the Langmuir models; the adsorption capacity of CA-BMB (1210.7 mg g-1) was close to that of CA (1282.2 mg g-1) and much higher than that of BMB alone (184.1 mg g-1).
30301348	0	0	theme	Tissue	119:124	arg1	Engineering					126:136	Bone Tissue Engineering	114:136	Bone Tissue Engineering	114:136	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	6	1	theme	scaffold	1082:1089	arg1	shape					1055:1059	the shape	1051:1059	the shape of the pure alginate scaffold	1051:1089	Consequently, the shape of the pure alginate scaffold was soft and easy to collapse contrarily to the composite scaffold.
30301348	6	1	theme	scaffold	1082:1089	arg1	soft					1095:1098	soft	1095:1098	soft	1095:1098	Consequently, the shape of the pure alginate scaffold was soft and easy to collapse contrarily to the composite scaffold.
30301348	7	2	theme	human	1285:1289	arg1	plasma					1297:1302	human blood plasma	1285:1302	human blood plasma	1285:1302	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	8	3	from	scaffolds	1426:1434	arg1	terms					1439:1443	terms	1439:1443	terms of compressive strength analysis	1439:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	0	4	theme	Bone	114:117	arg1	Engineering					126:136	Bone Tissue Engineering	114:136	Bone Tissue Engineering	114:136	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	8	5	theme	analysis	1469:1476	arg1	terms					1439:1443	terms	1439:1443	terms of compressive strength analysis	1439:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	4	6	theme	behavior	750:757	arg1	terms					729:733	terms	729:733	terms of thixotropic behavior and viscosity recovery of hydrogels	729:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	9	7	theme	alginate/TEMPO-oxidized	1542:1564	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	7	theme	alginate/TEMPO-oxidized	1542:1564	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	1	8	theme	filaments	399:407	arg1	collapse					383:390	the collapse	379:390	the collapse of the filaments	379:407	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	8	9	theme	strength	1460:1467	arg1	analysis					1469:1476	compressive strength analysis	1448:1476	compressive strength analysis	1448:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	3	10	theme	prepared	599:606	arg1	pastes					608:613	the prepared pastes	595:613	the prepared pastes	595:613	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	8	11	theme	scaffolds	1426:1434	arg1	properties					1397:1406	The strength properties	1384:1406	The strength properties of the fabricated scaffolds in terms of compressive strength analysis	1384:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	9	12	theme	bone	1638:1641	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	4	13	theme	steady	827:832	arg1	experiments					845:855	steady shear rate experiments	827:855	steady shear rate experiments	827:855	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	7	14	theme	blood	1291:1295	arg1	plasma					1297:1302	human blood plasma	1285:1302	human blood plasma	1285:1302	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	3	15	theme	pastes	608:613	arg1	composition					580:590	The composition	576:590	The composition of the prepared pastes	576:613	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	5	16	theme	alginate	912:919	arg1	hydrogel					921:928	pure alginate hydrogel	907:928	pure alginate hydrogel	907:928	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	7	17	theme	body	1234:1237	arg1	fluid					1239:1243	simulated body fluid	1224:1243	simulated body fluid	1224:1243	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	5	18	theme	value	964:968	arg1	%					947:947	only about 16%	934:947	only about 16% of the initial value	934:968	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	5	18	theme	value	964:968	arg1	value					964:968	the initial value	952:968	the initial value	952:968	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	5	18	theme	value	964:968	arg1	recovery					894:901	the viscosity recovery	880:901	the viscosity recovery for pure alginate hydrogel	880:928	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	7	19	theme	scaffolds	1208:1216	arg1	process					1189:1195	The biomimetic mineralization process	1159:1195	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma,	1159:1303	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	5	20	theme	pure	907:910	arg1	hydrogel					921:928	pure alginate hydrogel	907:928	pure alginate hydrogel	907:928	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	1	21	theme	partial	201:207	arg1	cross-linking					209:221	partial cross-linking	201:221	partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel	201:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	7	22	theme	mineralization	1174:1187	arg1	process					1189:1195	The biomimetic mineralization process	1159:1195	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma,	1159:1303	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	5	23	theme	initial	956:962	arg1	value					964:968	the initial value	952:968	the initial value	952:968	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	2	24	theme	hydrogel	534:541	arg1	rigidity					518:525	the rigidity	514:525	the rigidity of the hydrogel	514:541	The prepared scaffolds were fully cross-linked using calcium ions immediately after printing to provide the rigidity of the hydrogel and give it long-term stability.
30301348	4	25	theme	rate	840:843	arg1	experiments					845:855	steady shear rate experiments	827:855	steady shear rate experiments	827:855	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	4	26	from	properties	715:724	arg1	terms					729:733	terms	729:733	terms of thixotropic behavior and viscosity recovery of hydrogels	729:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	4	27	theme	rheological	703:713	arg1	properties					715:724	The rheological properties	699:724	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels	699:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	7	28	theme	inorganic	1260:1268	arg1	composition					1270:1280	the inorganic composition	1256:1280	the inorganic composition of human blood plasma	1256:1302	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	7	29	theme	hydroxyapatite	1327:1340	arg1	nucleation					1342:1351	the hydroxyapatite nucleation	1323:1351	the hydroxyapatite nucleation on the hydrogel	1323:1367	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	8	30	theme	fabricated	1415:1424	arg1	scaffolds					1426:1434	the fabricated scaffolds	1411:1434	the fabricated scaffolds in terms of compressive strength analysis	1411:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	8	31	from	terms	1439:1443	arg1	properties					1397:1406	The strength properties	1384:1406	The strength properties of the fabricated scaffolds in terms of compressive strength analysis	1384:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	0	32	theme	Biomimetic	0:9	arg1	Mineralization					11:24	Biomimetic Mineralization	0:24	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	0:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	9	33	theme	tissue	1643:1648	arg1	engineering					1650:1660	bone tissue engineering	1638:1660	bone tissue engineering	1638:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	4	34	theme	shear	834:838	arg1	experiments					845:855	steady shear rate experiments	827:855	steady shear rate experiments	827:855	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	1	35	theme	TEMPO-oxidized	226:239	arg1	hydrogel					271:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel	226:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel	226:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	0	36	theme	Three-Dimensional	29:45	arg1	Nanofibril					89:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	2	37	theme	prepared	414:421	arg1	scaffolds					423:431	The prepared scaffolds	410:431	The prepared scaffolds	410:431	The prepared scaffolds were fully cross-linked using calcium ions immediately after printing to provide the rigidity of the hydrogel and give it long-term stability.
30301348	7	38	theme	plasma	1297:1302	arg1	composition					1270:1280	the inorganic composition	1256:1280	the inorganic composition of human blood plasma	1256:1302	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	6	39	theme	alginate	1073:1080	arg1	scaffold					1082:1089	the pure alginate scaffold	1064:1089	the pure alginate scaffold	1064:1089	Consequently, the shape of the pure alginate scaffold was soft and easy to collapse contrarily to the composite scaffold.
30301348	1	40	theme	cellulose	241:249	arg1	hydrogel					271:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel	226:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel	226:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	6	41	theme	composite	1139:1147	arg1	scaffold					1149:1156	the composite scaffold	1135:1156	the composite scaffold	1135:1156	Consequently, the shape of the pure alginate scaffold was soft and easy to collapse contrarily to the composite scaffold.
30301348	1	42	theme	three-dimensional	143:159	arg1	scaffolds					174:182	The three-dimensional (3D) printed scaffolds	139:182	The three-dimensional (3D) printed scaffolds	139:182	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	9	43	theme	promising	1603:1611	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	43	theme	promising	1603:1611	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	1	44	theme	nanofibril/alginate	251:269	arg1	hydrogel					271:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel	226:278	TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel	226:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	0	45	theme	Alginate/TEMPO-Oxidized	55:77	arg1	Nanofibril					89:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	9	46	theme	cellulose	1566:1574	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	46	theme	cellulose	1566:1574	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	1	47	dep	printing	303:310	arg1	hydrogel					316:323	the hydrogel	312:323	printing the hydrogel	303:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	3	48	theme	description	643:653	arg1	view					631:634	view	631:634	view of the description of the hydrogel and 3D printing parameters	631:696	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	1	49	theme	hydrogel	271:278	arg1	cross-linking					209:221	partial cross-linking	201:221	partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel	201:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	0	50	theme	Printed	47:53	arg1	Nanofibril					89:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	3	51	theme	printing	678:685	arg1	parameters					687:696	the hydrogel and 3D printing parameters	658:696	parameters	687:696	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	5	52	theme	viscosity	884:892	arg1	%					947:947	only about 16%	934:947	only about 16% of the initial value	934:968	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	5	52	theme	viscosity	884:892	arg1	value					964:968	the initial value	952:968	the initial value	952:968	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	5	52	theme	viscosity	884:892	arg1	recovery					894:901	the viscosity recovery	880:901	the viscosity recovery for pure alginate hydrogel	880:928	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	2	53	theme	calcium	463:469	arg1	ions					471:474	calcium ions	463:474	calcium ions	463:474	The prepared scaffolds were fully cross-linked using calcium ions immediately after printing to provide the rigidity of the hydrogel and give it long-term stability.
30301348	0	54	theme	Nanofibril	89:98	arg1	Mineralization					11:24	Biomimetic Mineralization	0:24	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	0:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	8	55	theme	strength	1388:1395	arg1	properties					1397:1406	The strength properties	1384:1406	The strength properties of the fabricated scaffolds in terms of compressive strength analysis	1384:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	3	56	theme	parameters	687:696	arg1	description					643:653	the description	639:653	the description of the hydrogel and 3D printing parameters	639:696	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	5	57	theme	cellulose	1002:1010	arg1	nanofibrils					1012:1022	cellulose nanofibrils	1002:1022	cellulose nanofibrils	1002:1022	The results show that the viscosity recovery for pure alginate hydrogel was only about 16% of the initial value, whereas it was 66% when adding cellulose nanofibrils to alginate.
30301348	7	58	from	nucleation	1342:1351	arg1	hydrogel					1360:1367	the hydrogel	1356:1367	the hydrogel	1356:1367	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	4	59	theme	recovery	773:780	arg1	terms					729:733	terms	729:733	terms of thixotropic behavior and viscosity recovery of hydrogels	729:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	0	60	theme	Cellulose	79:87	arg1	Nanofibril					89:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril	29:98	Biomimetic Mineralization of Three-Dimensional Printed Alginate/TEMPO-Oxidized Cellulose Nanofibril Scaffolds for Bone Tissue Engineering.
30301348	9	61	theme	3D	1613:1614	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	61	theme	3D	1613:1614	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	8	62	theme	compressive	1448:1458	arg1	analysis					1469:1476	compressive strength analysis	1448:1476	compressive strength analysis	1448:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	4	63	theme	viscosity	763:771	arg1	recovery					773:780	viscosity recovery	763:780	viscosity recovery of hydrogels	763:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	7	64	theme	printed	1200:1206	arg1	scaffolds					1208:1216	printed scaffolds	1200:1216	printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma,	1200:1303	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	4	65	theme	hydrogels	785:793	arg1	behavior					750:757	thixotropic behavior	738:757	thixotropic behavior	738:757	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	4	65	theme	hydrogels	785:793	arg1	recovery					773:780	viscosity recovery	763:780	viscosity recovery of hydrogels	763:793	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	8	66	from	properties	1397:1406	arg1	terms					1439:1443	terms	1439:1443	terms of compressive strength analysis	1439:1476	The strength properties of the fabricated scaffolds in terms of compressive strength analysis were also investigated and discussed.
30301348	1	67	theme	calcium	286:292	arg1	ions					294:297	calcium ions	286:297	calcium ions for printing the hydrogel	286:323	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	3	68	theme	3D	675:676	arg1	parameters					687:696	the hydrogel and 3D printing parameters	658:696	parameters	687:696	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	1	69	theme	printed	166:172	arg1	scaffolds					174:182	The three-dimensional (3D) printed scaffolds	139:182	The three-dimensional (3D) printed scaffolds	139:182	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	9	70	theme	nanofibril	1576:1585	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	70	theme	nanofibril	1576:1585	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	2	71	theme	long-term	555:563	arg1	stability					565:573	long-term stability	555:573	long-term stability	555:573	The prepared scaffolds were fully cross-linked using calcium ions immediately after printing to provide the rigidity of the hydrogel and give it long-term stability.
30301348	6	72	theme	pure	1068:1071	arg1	scaffold					1082:1089	the pure alginate scaffold	1064:1089	the pure alginate scaffold	1064:1089	Consequently, the shape of the pure alginate scaffold was soft and easy to collapse contrarily to the composite scaffold.
30301348	4	73	theme	thixotropic	738:748	arg1	behavior					750:757	thixotropic behavior	738:757	thixotropic behavior	738:757	The rheological properties in terms of thixotropic behavior and viscosity recovery of hydrogels were investigated by performing steady shear rate experiments.
30301348	9	74	theme	printing	1616:1623	arg1	scaffold					1625:1632	a promising 3D printing scaffold	1601:1632	a promising 3D printing scaffold for bone tissue engineering	1601:1660	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	9	74	theme	printing	1616:1623	arg1	system					1587:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	the alginate/TEMPO-oxidized cellulose nanofibril system	1538:1592	The results show that the alginate/TEMPO-oxidized cellulose nanofibril system may be a promising 3D printing scaffold for bone tissue engineering.
30301348	7	75	theme	biomimetic	1163:1172	arg1	process					1189:1195	The biomimetic mineralization process	1159:1195	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma,	1159:1303	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
30301348	3	76	theme	hydrogel	662:669	arg1	description					643:653	the description	639:653	the description of the hydrogel and 3D printing parameters	639:696	The composition of the prepared pastes was adjusted in view of the description of the hydrogel and 3D printing parameters.
30301348	1	77	dep	three-dimensional	143:159	arg1	3D					162:163	3D	162:163	3D	162:163	The three-dimensional (3D) printed scaffolds were prepared by partial cross-linking of TEMPO-oxidized cellulose nanofibril/alginate hydrogel using calcium ions for printing the hydrogel while maintaining its shape, fidelity, and preventing the collapse of the filaments.
30301348	7	78	theme	simulated	1224:1232	arg1	fluid					1239:1243	simulated body fluid	1224:1243	simulated body fluid	1224:1243	The biomimetic mineralization process of printed scaffolds using simulated body fluid, mimicking the inorganic composition of human blood plasma, was performed and the hydroxyapatite nucleation on the hydrogel was confirmed.
31344416	1	0	theme	different	168:176	arg1	environments					199:210	different Tunisian hypersaline environments	168:210	different Tunisian hypersaline environments	168:210	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	2	1	theme	different	372:380	arg1	M2					420:421	M2	420:421	M2 (semi-complex medium)	420:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	1	theme	different	372:380	arg1	media					390:394	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	1	theme	different	372:380	arg1	M1					396:397	M1	396:397	M1 (complex medium)	396:414	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	0	2	theme	hypersaline	101:111	arg1	environments					113:124	Tunisian hypersaline environments	92:124	Tunisian hypersaline environments	92:124	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	0	3	theme	Tunisian	92:99	arg1	environments					113:124	Tunisian hypersaline environments	92:124	Tunisian hypersaline environments	92:124	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	2	4	theme	culture	382:388	arg1	M2					420:421	M2	420:421	M2 (semi-complex medium)	420:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	4	theme	culture	382:388	arg1	media					390:394	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	4	theme	culture	382:388	arg1	M1					396:397	M1	396:397	M1 (complex medium)	396:414	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	6	5	theme	monosaccharide	850:863	arg1	profile					865:871	almost the same monosaccharide profile	834:871	almost the same monosaccharide profile	834:871	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	4	6	theme	stronger	610:617	arg1	growth					629:634	stronger bacterial growth	610:634	stronger bacterial growth associated with greater EPS production compared to M2	610:688	The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2.
31344416	1	7	theme	hypersaline	187:197	arg1	environments					199:210	different Tunisian hypersaline environments	168:210	different Tunisian hypersaline environments	168:210	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	6	8	theme	same	845:848	arg1	profile					865:871	almost the same monosaccharide profile	834:871	almost the same monosaccharide profile	834:871	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	2	9	dep	media	390:394	arg1	medium					408:413	complex medium	400:413	complex medium	400:413	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	9	dep	media	390:394	arg1	M2					420:421	M2	420:421	M2 (semi-complex medium)	420:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	9	dep	media	390:394	arg1	medium					437:442	semi-complex medium	424:442	semi-complex medium	424:442	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	9	dep	media	390:394	arg1	media					390:394	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	two different culture media M1 (complex medium) and M2 (semi-complex medium)	368:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	9	dep	media	390:394	arg1	M1					396:397	M1	396:397	M1 (complex medium)	396:414	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	3	10	theme	Selected	446:453	arg1	isolates					455:462	Selected isolates	446:462	Selected isolates	446:462	Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively.
31344416	7	11	theme	further	1137:1143	arg1	applications					1145:1156	many further applications	1132:1156	many further applications	1132:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	2	12	theme	complex	400:406	arg1	medium					408:413	complex medium	400:413	complex medium	400:413	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	12	theme	complex	400:406	arg1	M1					396:397	M1	396:397	M1 (complex medium)	396:414	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	1	13	theme	16S	299:301	arg1	sequencing					313:322	16S rRNA gene sequencing	299:322	16S rRNA gene sequencing	299:322	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	3	14	dep	170 mg L-1	514:523	arg1	to					511:512	to	511:512	to	511:512	Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively.
31344416	4	15	theme	EPS	660:662	arg1	production					664:673	greater EPS production	652:673	greater EPS production	652:673	The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2.
31344416	0	16	theme	production	18:27	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.	0:125	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	1	17	theme	rRNA	303:306	arg1	sequencing					313:322	16S rRNA gene sequencing	299:322	16S rRNA gene sequencing	299:322	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	7	18	theme	different	1036:1044	arg1	species					1046:1052	the different species	1032:1052	the different species	1032:1052	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	2	19	theme	EPS	325:327	arg1	production					329:338	EPS production	325:338	EPS production	325:338	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	20	theme	semi-complex	424:435	arg1	M2					420:421	M2	420:421	M2 (semi-complex medium)	420:443	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	2	20	theme	semi-complex	424:435	arg1	medium					437:442	semi-complex medium	424:442	semi-complex medium	424:442	EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium).
31344416	7	21	theme	many	1132:1135	arg1	applications					1145:1156	many further applications	1132:1156	many further applications	1132:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	1	22	theme	gene	308:311	arg1	sequencing					313:322	16S rRNA gene sequencing	299:322	16S rRNA gene sequencing	299:322	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	1	23	theme	Tunisian	178:185	arg1	environments					199:210	different Tunisian hypersaline environments	168:210	different Tunisian hypersaline environments	168:210	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	3	24	theme	EPS	483:485	arg1	amounts					487:493	different EPS amounts	473:493	different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1	473:544	Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively.
31344416	0	25	theme	exopolysaccharides	32:49	arg1	production					18:27	the production	14:27	the production of exopolysaccharides	14:49	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	6	26	theme	main	919:922	arg1	monomers					924:931	the main monomers	915:931	the main monomers	915:931	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	6	26	theme	main	919:922	arg1	glucose					887:893	glucose	887:893	glucose	887:893	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	6	26	theme	main	919:922	arg1	arabinose					899:907	arabinose	899:907	arabinose	899:907	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	6	26	theme	main	919:922	arg1	mannose					878:884	mannose	878:884	mannose	878:884	When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers.
31344416	7	27	from	EPS	956:958	arg1	M2					963:964	M2	963:964	M2	963:964	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	1	28	theme	exopolysaccharides	234:251	arg1	production					220:229	the production	216:229	the production of exopolysaccharides (EPS)	216:257	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	4	29	theme	greater	652:658	arg1	production					664:673	greater EPS production	652:673	greater EPS production	652:673	The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2.
31344416	5	30	theme	highest	709:715	arg1	YEPS/X					728:733	YEPS/X	728:733	YEPS/X	728:733	Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2.
31344416	5	30	theme	highest	709:715	arg1	yield					721:725	the highest EPS yield	705:725	the highest EPS yield (YEPS/X)	705:734	Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2.
31344416	5	31	theme	EPS	717:719	arg1	YEPS/X					728:733	YEPS/X	728:733	YEPS/X	728:733	Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2.
31344416	5	31	theme	EPS	717:719	arg1	yield					721:725	the highest EPS yield	705:725	the highest EPS yield (YEPS/X)	705:734	Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2.
31344416	7	32	theme	monosaccharide	1002:1015	arg1	profiles					1017:1024	heterogeneous monosaccharide profiles	988:1024	heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications	988:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	7	33	theme	versatile	1104:1112	arg1	material					1114:1121	a versatile material	1102:1121	a versatile material used for many further applications	1102:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	7	33	theme	versatile	1104:1112	arg1	glucomannan					1076:1086	glucomannan	1076:1086	glucomannan that could be a versatile material used for many further applications	1076:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	1	34	theme	Halomonas	131:139	arg1	strains					141:147	Ten Halomonas strains	127:147	Ten Halomonas strains	127:147	Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing.
31344416	0	35	from	environments	113:124	arg1	strains					79:85	newly isolated Halomonas strains	54:85	newly isolated Halomonas strains from Tunisian hypersaline environments	54:124	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	7	36	theme	heterogeneous	988:1000	arg1	profiles					1017:1024	heterogeneous monosaccharide profiles	988:1024	heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications	988:1156	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	7	37	theme	produced	947:954	arg1	EPS					956:958	the produced EPS	943:958	the produced EPS on M2	943:964	However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
31344416	0	38	theme	Halomonas	69:77	arg1	strains					79:85	newly isolated Halomonas strains	54:85	newly isolated Halomonas strains from Tunisian hypersaline environments	54:124	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	3	39	theme	different	473:481	arg1	amounts					487:493	different EPS amounts	473:493	different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1	473:544	Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively.
31344416	0	40	theme	isolated	60:67	arg1	strains					79:85	newly isolated Halomonas strains	54:85	newly isolated Halomonas strains from Tunisian hypersaline environments	54:124	Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
31344416	4	41	theme	bacterial	619:627	arg1	growth					629:634	stronger bacterial growth	610:634	stronger bacterial growth associated with greater EPS production compared to M2	610:688	The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2.
31344416	4	42	theme	M1	596:597	arg1	use					589:591	The use	585:591	The use of M1	585:597	The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2.
30513582	5	0	theme	cancer	1055:1060	arg1	immunotherapy					1062:1074	cancer immunotherapy	1055:1074	cancer immunotherapy	1055:1074	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	4	1	theme	TAMs	827:830	arg1	function					815:822	the function	811:822	the function of TAMs	811:830	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	1	2	theme	acidic	153:158	arg1	IAPS-2					183:188	IAPS-2	183:188	namely IAPS-2	176:188	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	1	2	theme	acidic	153:158	arg1	polysaccharide					160:173	an acidic polysaccharide	150:173	an acidic polysaccharide	150:173	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	1	3	theme	anti-tumor	228:237	arg1	activity					239:246	anti-tumor activity	228:246	anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype	228:311	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	2	4	theme	cytokines	466:474	arg1	secretion					442:450	the secretion	438:450	the secretion of anti-tumor cytokines in macrophages	438:489	We obtained IAPS-2 polysaccharide from this herb based on acidity and found that IAPS-2 expressed the activity of promoting the secretion of anti-tumor cytokines in macrophages.
30513582	5	5	theme	action	993:998	arg1	mechanism					980:988	a solid mechanism	972:988	a solid mechanism of action	972:998	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	5	theme	action	993:998	arg1	activity					958:965	potent anti-tumor activity	940:965	potent anti-tumor activity	940:965	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	5	theme	action	993:998	arg1	composition					927:937	a clearly illustrated chemical composition	896:937	a clearly illustrated chemical composition	896:937	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	1	6	theme	polysaccharide	160:173	arg1	discovery					137:145	the discovery	133:145	the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella	133:220	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	3	7	from	effect	533:538	arg1	cells					547:551	TAM cells	543:551	TAM cells	543:551	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	0	8	theme	Sarcoma	95:101	arg1	Immunotherapy					103:115	Sarcoma Immunotherapy	95:115	Sarcoma Immunotherapy	95:115	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	2	9	from	secretion	442:450	arg1	macrophages					479:489	macrophages	479:489	macrophages	479:489	We obtained IAPS-2 polysaccharide from this herb based on acidity and found that IAPS-2 expressed the activity of promoting the secretion of anti-tumor cytokines in macrophages.
30513582	5	10	theme	illustrated	906:916	arg1	composition					927:937	a clearly illustrated chemical composition	896:937	a clearly illustrated chemical composition	896:937	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	11	theme	valuable	1025:1032	arg1	tool					1046:1049	a valuable therapeutic tool	1023:1049	a valuable therapeutic tool for cancer immunotherapy	1023:1074	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	12	theme	chemical	918:925	arg1	composition					927:937	a clearly illustrated chemical composition	896:937	a clearly illustrated chemical composition	896:937	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	13	theme	therapeutic	1034:1044	arg1	tool					1046:1049	a valuable therapeutic tool	1023:1049	a valuable therapeutic tool for cancer immunotherapy	1023:1074	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	3	14	theme	anti-tumor	522:531	arg1	effect					533:538	its anti-tumor effect	518:538	its anti-tumor effect on TAM cells	518:551	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	0	15	theme	Tumor	14:18	arg1	Phenotype					42:50	Tumor Associated Macrophage Phenotype	14:50	Tumor Associated Macrophage Phenotype	14:50	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	5	16	theme	anti-tumor	947:956	arg1	activity					958:965	potent anti-tumor activity	940:965	potent anti-tumor activity	940:965	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	4	17	theme	tumor	699:703	arg1	model					712:716	the tumor murine model	695:716	the tumor murine model	695:716	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	1	18	theme	repolarizing	254:265	arg1	TAMs					297:300	TAMs	297:300	TAMs	297:300	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	1	18	theme	repolarizing	254:265	arg1	macrophages					284:294	repolarizing tumor associated macrophages	254:294	a repolarizing tumor associated macrophages (TAMs) phenotype	252:311	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	0	19	theme	Macrophage	31:40	arg1	Phenotype					42:50	Tumor Associated Macrophage Phenotype	14:50	Tumor Associated Macrophage Phenotype	14:50	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	1	20	theme	tumor	267:271	arg1	TAMs					297:300	TAMs	297:300	TAMs	297:300	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	1	20	theme	tumor	267:271	arg1	macrophages					284:294	repolarizing tumor associated macrophages	254:294	a repolarizing tumor associated macrophages (TAMs) phenotype	252:311	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	3	21	theme	TAM	543:545	arg1	cells					547:551	TAM cells	543:551	TAM cells	543:551	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	0	22	theme	Associated	20:29	arg1	Phenotype					42:50	Tumor Associated Macrophage Phenotype	14:50	Tumor Associated Macrophage Phenotype	14:50	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	1	23	theme	associated	273:282	arg1	TAMs					297:300	TAMs	297:300	TAMs	297:300	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	1	23	theme	associated	273:282	arg1	macrophages					284:294	repolarizing tumor associated macrophages	254:294	a repolarizing tumor associated macrophages (TAMs) phenotype	252:311	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	3	24	theme	transducer	617:626	arg1	signaling					666:674	signal transducer and activator of transcription (STAT) signaling	610:674	signal transducer and activator of transcription (STAT) signaling	610:674	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	4	25	theme	murine	705:710	arg1	model					712:716	the tumor murine model	695:716	the tumor murine model	695:716	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	2	26	theme	anti-tumor	455:464	arg1	cytokines					466:474	anti-tumor cytokines	455:474	anti-tumor cytokines	455:474	We obtained IAPS-2 polysaccharide from this herb based on acidity and found that IAPS-2 expressed the activity of promoting the secretion of anti-tumor cytokines in macrophages.
30513582	1	27	theme	macrophages	284:294	arg1	phenotype					303:311	a repolarizing tumor associated macrophages (TAMs) phenotype	252:311	a repolarizing tumor associated macrophages (TAMs) phenotype	252:311	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	0	28	from	asprella	82:89	arg1	Polysaccharide					57:70	a Polysaccharide	55:70	a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy	55:115	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	5	29	with	IAPS-2	883:888	arg1	mechanism					980:988	a solid mechanism	972:988	a solid mechanism of action	972:998	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	29	with	IAPS-2	883:888	arg1	activity					958:965	potent anti-tumor activity	940:965	potent anti-tumor activity	940:965	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	5	29	with	IAPS-2	883:888	arg1	composition					927:937	a clearly illustrated chemical composition	896:937	a clearly illustrated chemical composition	896:937	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	3	30	theme	nuclear	580:586	arg1	NF-κB					599:603	NF-κB	599:603	NF-κB	599:603	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	3	30	theme	nuclear	580:586	arg1	factor-κB					588:596	nuclear factor-κB	580:596	nuclear factor-κB (NF-κB)	580:604	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	1	31	from	root	200:203	arg1	discovery					137:145	the discovery	133:145	the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella	133:220	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	4	32	theme	animal	849:854	arg1	rate					865:868	the animal survival rate	845:868	the animal survival rate	845:868	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	3	33	theme	signal	610:615	arg1	transducer					617:626	signal transducer	610:626	signal transducer	610:626	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	3	33	theme	signal	610:615	arg1	STAT					660:663	STAT	660:663	STAT	660:663	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	4	34	theme	tumors	789:794	arg1	growth					779:784	the growth	775:784	the growth of tumors	775:794	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	2	35	theme	IAPS-2	326:331	arg1	polysaccharide					333:346	IAPS-2 polysaccharide	326:346	IAPS-2 polysaccharide	326:346	We obtained IAPS-2 polysaccharide from this herb based on acidity and found that IAPS-2 expressed the activity of promoting the secretion of anti-tumor cytokines in macrophages.
30513582	3	36	theme	transcription	645:657	arg1	transducer					617:626	signal transducer	610:626	signal transducer	610:626	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	3	36	theme	transcription	645:657	arg1	STAT					660:663	STAT	660:663	STAT	660:663	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	3	36	theme	transcription	645:657	arg1	activator					632:640	activator	632:640	activator	632:640	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	5	37	theme	solid	974:978	arg1	mechanism					980:988	a solid mechanism	972:988	a solid mechanism of action	972:998	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	1	38	theme	Ilex	208:211	arg1	asprella					213:220	Ilex asprella	208:220	Ilex asprella	208:220	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	4	39	theme	survival	856:863	arg1	rate					865:868	the animal survival rate	845:868	the animal survival rate	845:868	In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate.
30513582	3	40	theme	activator	632:640	arg1	signaling					666:674	signal transducer and activator of transcription (STAT) signaling	610:674	signal transducer and activator of transcription (STAT) signaling	610:674	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	5	41	theme	potent	940:945	arg1	activity					958:965	potent anti-tumor activity	940:965	potent anti-tumor activity	940:965	In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
30513582	1	42	theme	asprella	213:220	arg1	root					200:203	the root	196:203	the root of Ilex asprella	196:220	We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of Ilex asprella, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype.
30513582	0	43	theme	Ilex	77:80	arg1	asprella					82:89	Ilex asprella	77:89	Ilex asprella for Sarcoma Immunotherapy	77:115	Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy.
30513582	3	44	theme	factor-κB	588:596	arg1	activation					566:575	the activation	562:575	the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling	562:674	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
30513582	3	45	theme	signaling	666:674	arg1	activation					566:575	the activation	562:575	the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling	562:674	Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling.
31320071	4	0	theme	attractions	564:574	arg1	shielding					537:545	shielding	537:545	shielding of electrostatic attractions between these two oppositely charged biomaterials	537:624	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	0	theme	attractions	564:574	arg1	solution					662:669	obtaining concentrated collagen solution	630:669	obtaining concentrated collagen solution	630:669	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	7	1	theme	collagen	1320:1327	arg1	solution					1329:1336	concentrated collagen solution	1307:1336	concentrated collagen solution with DCMC	1307:1346	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	6	2	theme	hydrogel	1134:1141	arg1	composites					1143:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	6	3	theme	commendable	1162:1172	arg1	biocompatibility					1174:1189	commendable biocompatibility	1162:1189	commendable biocompatibility	1162:1189	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	0	4	theme	carboxymethyl	78:90	arg1	cellulose					92:100	dialdehyde carboxymethyl cellulose	67:100	dialdehyde carboxymethyl cellulose	67:100	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	1	5	theme	strong	169:174	arg1	microenvironment					183:198	a mechanically strong matrix microenvironment	154:198	a mechanically strong matrix microenvironment	154:198	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	6	6	theme	collagen	1125:1132	arg1	composites					1143:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	0	7	from	collagen	54:61	arg1	system					124:129	a biphasic solvent system	105:129	a biphasic solvent system	105:129	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	7	8	theme	concentrated	1307:1318	arg1	solution					1329:1336	concentrated collagen solution	1307:1336	concentrated collagen solution with DCMC	1307:1346	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	5	9	theme	thermal	953:959	arg1	temperature					974:984	thermal denaturation temperature	953:984	thermal denaturation temperature	953:984	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	0	10	theme	solvent	116:122	arg1	system					124:129	a biphasic solvent system	105:129	a biphasic solvent system	105:129	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	7	11	theme	solvent	1362:1368	arg1	system					1370:1375	a biphasic solvent system	1351:1375	a biphasic solvent system	1351:1375	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	2	12	theme	chemical	314:321	arg1	crosslinkers					323:334	chemical crosslinkers	314:334	chemical crosslinkers for rapid network formation	314:362	Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation.
31320071	1	13	theme	matrix	176:181	arg1	microenvironment					183:198	a mechanically strong matrix microenvironment	154:198	a mechanically strong matrix microenvironment	154:198	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	0	14	theme	biphasic	107:114	arg1	system					124:129	a biphasic solvent system	105:129	a biphasic solvent system	105:129	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	4	15	theme	electrostatic	550:562	arg1	attractions					564:574	electrostatic attractions	550:574	electrostatic attractions between these two oppositely charged biomaterials	550:624	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	1	16	with	hydrogels	139:147	arg1	microenvironment					183:198	a mechanically strong matrix microenvironment	154:198	a mechanically strong matrix microenvironment	154:198	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	7	17	with	solution	1329:1336	arg1	DCMC					1343:1346	DCMC	1343:1346	DCMC	1343:1346	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	7	18	theme	biocompatible	1261:1273	arg1	composites					1284:1293	stable and biocompatible hydrogel composites	1250:1293	stable and biocompatible hydrogel composites	1250:1293	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	3	19	theme	dialdehyde	373:382	arg1	reagent					455:461	an optimal crosslinking reagent	431:461	an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity	431:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	19	theme	dialdehyde	373:382	arg1	DCMC					409:412	DCMC	409:412	DCMC	409:412	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	19	theme	dialdehyde	373:382	arg1	cellulose					398:406	dialdehyde carboxymethyl cellulose	373:406	dialdehyde carboxymethyl cellulose (DCMC)	373:413	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	5	20	theme	anti-enzymatic	987:1000	arg1	ability					1002:1008	anti-enzymatic ability	987:1008	anti-enzymatic ability	987:1008	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	0	21	theme	Stable	0:5	arg1	composites					34:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	4	22	theme	concentrated	640:651	arg1	solution					662:669	obtaining concentrated collagen solution	630:669	obtaining concentrated collagen solution	630:669	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	7	23	theme	hydrogel	1275:1282	arg1	composites					1284:1293	stable and biocompatible hydrogel composites	1250:1293	stable and biocompatible hydrogel composites	1250:1293	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	1	24	theme	favorable	204:212	arg1	hydrogels					139:147	Stable hydrogels	132:147	Stable hydrogels with a mechanically strong matrix microenvironment	132:198	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	1	24	theme	favorable	204:212	arg1	biomaterials					214:225	favorable biomaterials	204:225	favorable biomaterials for three-dimensional cell culture	204:260	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	0	25	theme	hydrogel	25:32	arg1	composites					34:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	4	26	theme	obtaining	630:638	arg1	solution					662:669	obtaining concentrated collagen solution	630:669	obtaining concentrated collagen solution	630:669	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	6	27	theme	obtained	1099:1106	arg1	composites					1143:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	6	28	link	DCMC-crosslinked	1108:1123	arg1	composites					1143:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	2	29	theme	network	346:352	arg1	formation					354:362	rapid network formation	340:362	rapid network formation	340:362	Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation.
31320071	4	30	dep	AA/[EMIM	757:764	arg1	[Ac					766:768	[Ac	766:768	[Ac	766:768	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	31	theme	acetate	748:754	arg1	system					780:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	0	32	theme	biocompatible	11:23	arg1	composites					34:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites	0:43	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	5	33	contain	containing	808:817	arg2	crosslinks					824:833	more crosslinks	819:833	more crosslinks	819:833	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	5	33	contain	containing	808:817	arg1	composites					797:806	Hydrogel composites	788:806	Hydrogel composites containing more crosslinks	788:833	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	0	34	from	cellulose	92:100	arg1	system					124:129	a biphasic solvent system	105:129	a biphasic solvent system	105:129	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	2	35	theme	rapid	340:344	arg1	formation					354:362	rapid network formation	340:362	rapid network formation	340:362	Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation.
31320071	6	36	theme	cell	1048:1051	arg1	assay					1067:1071	cell proliferation assay	1048:1071	cell proliferation assay	1048:1071	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	4	37	theme	/1-ethyl-3-methylimidazolim	720:746	arg1	AA/[EMIM					757:764	AA/[EMIM	757:764	AA/[EMIM	757:764	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	37	theme	/1-ethyl-3-methylimidazolim	720:746	arg1	acetate					748:754	acetic acid /1-ethyl-3-methylimidazolim acetate	708:754	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	38	theme	collagen	653:660	arg1	solution					662:669	obtaining concentrated collagen solution	630:669	obtaining concentrated collagen solution	630:669	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	5	39	theme	more	819:822	arg1	crosslinks					824:833	more crosslinks	819:833	more crosslinks	819:833	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	3	40	theme	suitable	502:509	arg1	reactivity					520:529	suitable chemical reactivity	502:529	suitable chemical reactivity	502:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	2	41	theme	collagen	270:277	arg1	solution					279:286	Acidic collagen solution	263:286	Acidic collagen solution	263:286	Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation.
31320071	3	42	theme	excellent	471:479	arg1	biocompatibility					481:496	its excellent biocompatibility	467:496	its excellent biocompatibility	467:496	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	1	43	theme	three-dimensional	231:247	arg1	culture					254:260	three-dimensional cell culture	231:260	three-dimensional cell culture	231:260	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	5	44	theme	collagen	863:870	arg1	concentrations					872:885	collagen concentrations	863:885	collagen concentrations (5-25 mg/mL)	863:898	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	5	44	theme	collagen	863:870	arg1	5-25 mg/mL					888:897	5-25 mg/mL	888:897	5-25 mg/mL	888:897	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	4	45	theme	charged	605:611	arg1	biomaterials					613:624	these two oppositely charged biomaterials	584:624	these two oppositely charged biomaterials	584:624	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	2	46	theme	Acidic	263:268	arg1	solution					279:286	Acidic collagen solution	263:286	Acidic collagen solution	263:286	Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation.
31320071	5	47	theme	denaturation	961:972	arg1	temperature					974:984	thermal denaturation temperature	953:984	thermal denaturation temperature	953:984	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	1	48	theme	cell	249:252	arg1	culture					254:260	three-dimensional cell culture	231:260	three-dimensional cell culture	231:260	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	3	49	theme	optimal	434:440	arg1	reagent					455:461	an optimal crosslinking reagent	431:461	an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity	431:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	49	theme	optimal	434:440	arg1	cellulose					398:406	dialdehyde carboxymethyl cellulose	373:406	dialdehyde carboxymethyl cellulose (DCMC)	373:413	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	7	50	theme	promising	1214:1222	arg1	strategy					1224:1231	a promising strategy	1212:1231	a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system	1212:1375	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	6	51	theme	DCMC-crosslinked	1108:1123	arg1	composites					1143:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	all the obtained DCMC-crosslinked collagen hydrogel composites	1091:1152	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	3	52	theme	crosslinking	442:453	arg1	reagent					455:461	an optimal crosslinking reagent	431:461	an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity	431:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	52	theme	crosslinking	442:453	arg1	cellulose					398:406	dialdehyde carboxymethyl cellulose	373:406	dialdehyde carboxymethyl cellulose (DCMC)	373:413	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	7	53	theme	stable	1250:1255	arg1	composites					1284:1293	stable and biocompatible hydrogel composites	1250:1293	stable and biocompatible hydrogel composites	1250:1293	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	5	54	theme	improved	921:928	arg1	properties					941:950	the improved mechanical properties	917:950	the improved mechanical properties	917:950	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	7	55	theme	biphasic	1353:1360	arg1	system					1370:1375	a biphasic solvent system	1351:1375	a biphasic solvent system	1351:1375	This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.
31320071	1	56	theme	Stable	132:137	arg1	hydrogels					139:147	Stable hydrogels	132:147	Stable hydrogels with a mechanically strong matrix microenvironment	132:198	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	1	56	theme	Stable	132:137	arg1	biomaterials					214:225	favorable biomaterials	204:225	favorable biomaterials for three-dimensional cell culture	204:260	Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture.
31320071	4	57	theme	biphasic	699:706	arg1	system					780:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	5	58	theme	Hydrogel	788:795	arg1	composites					797:806	Hydrogel composites	788:806	Hydrogel composites containing more crosslinks	788:833	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	5	59	theme	compact	1014:1020	arg1	microstructure					1022:1035	compact microstructure	1014:1035	compact microstructure	1014:1035	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	4	60	theme	novel	693:697	arg1	system					780:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	0	61	theme	dialdehyde	67:76	arg1	cellulose					92:100	dialdehyde carboxymethyl cellulose	67:100	dialdehyde carboxymethyl cellulose	67:100	Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
31320071	3	62	theme	chemical	511:518	arg1	reactivity					520:529	suitable chemical reactivity	502:529	suitable chemical reactivity	502:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	4	63	theme	acid	715:718	arg1	AA/[EMIM					757:764	AA/[EMIM	757:764	AA/[EMIM	757:764	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	63	theme	acid	715:718	arg1	acetate					748:754	acetic acid /1-ethyl-3-methylimidazolim acetate	708:754	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	3	64	theme	carboxymethyl	384:396	arg1	reagent					455:461	an optimal crosslinking reagent	431:461	an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity	431:529	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	64	theme	carboxymethyl	384:396	arg1	DCMC					409:412	DCMC	409:412	DCMC	409:412	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	3	64	theme	carboxymethyl	384:396	arg1	cellulose					398:406	dialdehyde carboxymethyl cellulose	373:406	dialdehyde carboxymethyl cellulose (DCMC)	373:413	Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity.
31320071	6	65	theme	proliferation	1053:1065	arg1	assay					1067:1071	cell proliferation assay	1048:1071	cell proliferation assay	1048:1071	Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility.
31320071	5	66	theme	mechanical	930:939	arg1	properties					941:950	the improved mechanical properties	917:950	the improved mechanical properties	917:950	Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5-25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure.
31320071	4	67	theme	solvent	772:778	arg1	system					780:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	68	theme	acetic	708:713	arg1	AA/[EMIM					757:764	AA/[EMIM	757:764	AA/[EMIM	757:764	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31320071	4	68	theme	acetic	708:713	arg1	acetate					748:754	acetic acid /1-ethyl-3-methylimidazolim acetate	708:754	a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system	691:785	Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system.
31730988	3	0	theme	Sodium	334:339	arg1	oxidation					351:359	Sodium periodate oxidation	334:359	Sodium periodate oxidation process	334:367	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	5	1	theme	field	667:671	arg1	FESEM					712:716	FESEM	712:716	FESEM	712:716	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	1	theme	field	667:671	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	1	theme	field	667:671	arg1	microscope					700:709	field emission scanning electron microscope	667:709	field emission scanning electron microscope (FESEM)	667:717	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	0	2	theme	antibacterial	21:33	arg1	effect					35:40	antibacterial effect	21:40	antibacterial effect	21:40	Characterization and antibacterial effect of quaternized chitosan anchored cellulose beads.
31730988	3	3	theme	periodate	341:349	arg1	oxidation					351:359	Sodium periodate oxidation	334:359	Sodium periodate oxidation process	334:367	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	5	4	theme	emission	673:680	arg1	FESEM					712:716	FESEM	712:716	FESEM	712:716	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	4	theme	emission	673:680	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	4	theme	emission	673:680	arg1	microscope					700:709	field emission scanning electron microscope	667:709	field emission scanning electron microscope (FESEM)	667:717	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	4	5	theme	base	539:542	arg1	reaction					544:551	Schiff base reaction	532:551	Schiff base reaction	532:551	HTCC was anchored on the beads through Schiff base reaction.
31730988	5	6	theme	scanning	682:689	arg1	FESEM					712:716	FESEM	712:716	FESEM	712:716	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	6	theme	scanning	682:689	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	6	theme	scanning	682:689	arg1	microscope					700:709	field emission scanning electron microscope	667:709	field emission scanning electron microscope (FESEM)	667:717	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	6	7	theme	decomposition	958:970	arg1	temperature					972:982	the decomposition temperature	954:982	the decomposition temperature of the beads	954:995	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	5	8	theme	electron	691:698	arg1	FESEM					712:716	FESEM	712:716	FESEM	712:716	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	8	theme	electron	691:698	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	8	theme	electron	691:698	arg1	microscope					700:709	field emission scanning electron microscope	667:709	field emission scanning electron microscope (FESEM)	667:717	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	6	9	theme	thermogravimetric	877:893	arg1	TGA					905:907	TGA	905:907	TGA	905:907	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	6	9	theme	thermogravimetric	877:893	arg1	analysis					895:902	thermogravimetric analysis	877:902	thermogravimetric analysis (TGA)	877:908	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	5	10	theme	FTIR	652:655	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	10	theme	FTIR	652:655	arg1	magnetometer					809:820	vibrating sample magnetometer	792:820	vibrating sample magnetometer (VSM)	792:826	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	10	theme	FTIR	652:655	arg1	spectroscopy					744:755	energy dispersive X-ray spectroscopy	720:755	energy dispersive X-ray spectroscopy (EDS)	720:761	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	10	theme	FTIR	652:655	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction (XRD)	764:786	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	10	theme	FTIR	652:655	arg1	microscope					700:709	field emission scanning electron microscope	667:709	field emission scanning electron microscope (FESEM)	667:717	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	11	dep	anchored	591:598	arg1	HTCC					586:589	HTCC	586:589	HTCC	586:589	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	12	theme	vibrating	792:800	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	12	theme	vibrating	792:800	arg1	magnetometer					809:820	vibrating sample magnetometer	792:820	vibrating sample magnetometer (VSM)	792:826	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	12	theme	vibrating	792:800	arg1	VSM					823:825	VSM	823:825	VSM	823:825	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	3	13	theme	regenerated	429:439	arg1	beads					451:455	the regenerated cellulose beads	425:455	the regenerated cellulose beads mixed with maghemite nanoparticles	425:490	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	5	14	theme	beads	600:604	arg1	structure					558:566	The structure	554:566	The structure	554:566	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	14	theme	beads	600:604	arg1	properties					572:581	properties	572:581	properties	572:581	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	7	15	dep	confirmed	1117:1125	arg1	caused					1127:1132	caused	1127:1132	were confirmed caused by the covalent bond between HTCC and the beads	1112:1180	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads.
31730988	3	16	theme	maghemite	468:476	arg1	nanoparticles					478:490	maghemite nanoparticles	468:490	maghemite nanoparticles	468:490	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	3	17	theme	functional	404:413	arg1	groups					415:420	dialdehyde functional groups	393:420	dialdehyde functional groups	393:420	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	7	18	theme	beads	1065:1069	arg1	activities					1047:1056	A long-term antibacterial activities	1021:1056	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris	1021:1110	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads.
31730988	1	19	theme	thermoduric	97:107	arg1	spoilage					114:121	thermoduric food spoilage	97:121	Some thermoduric food spoilage bacteria	92:130	Some thermoduric food spoilage bacteria pose great threat to beverage industry.
31730988	5	20	theme	dispersive	727:736	arg1	EDS					758:760	EDS	758:760	EDS	758:760	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	20	theme	dispersive	727:736	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	20	theme	dispersive	727:736	arg1	spectroscopy					744:755	energy dispersive X-ray spectroscopy	720:755	energy dispersive X-ray spectroscopy (EDS)	720:761	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	8	21	theme	cellulose	1214:1222	arg1	beads					1224:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	1	22	theme	food	109:112	arg1	spoilage					114:121	thermoduric food spoilage	97:121	Some thermoduric food spoilage bacteria	92:130	Some thermoduric food spoilage bacteria pose great threat to beverage industry.
31730988	8	23	theme	novel	1244:1248	arg1	approach					1250:1257	a novel approach	1242:1257	a novel approach for food safety management	1242:1284	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	8	24	theme	HTCC	1201:1204	arg1	beads					1224:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	2	25	theme	dropping	313:320	arg1	technology					322:331	a dropping technology	311:331	a dropping technology	311:331	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	8	26	theme	food	1263:1266	arg1	management					1275:1284	food safety management	1263:1284	food safety management	1263:1284	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	3	27	theme	dialdehyde	393:402	arg1	groups					415:420	dialdehyde functional groups	393:420	dialdehyde functional groups	393:420	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	7	28	theme	antibacterial	1033:1045	arg1	activities					1047:1056	A long-term antibacterial activities	1021:1056	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris	1021:1110	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads.
31730988	5	29	theme	energy	720:725	arg1	EDS					758:760	EDS	758:760	EDS	758:760	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	29	theme	energy	720:725	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	29	theme	energy	720:725	arg1	spectroscopy					744:755	energy dispersive X-ray spectroscopy	720:755	energy dispersive X-ray spectroscopy (EDS)	720:761	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	0	30	theme	quaternized	45:55	arg1	chitosan					57:64	quaternized chitosan	45:64	quaternized chitosan	45:64	Characterization and antibacterial effect of quaternized chitosan anchored cellulose beads.
31730988	3	31	dep	oxidation	351:359	arg1	process					361:367	process	361:367	Sodium periodate oxidation process	334:367	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	6	32	theme	beads	854:858	arg1	stability					837:845	Thermal stability	829:845	Thermal stability of the beads	829:858	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	8	33	theme	biodegradable	1187:1199	arg1	beads					1224:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	3	34	theme	cellulose	441:449	arg1	beads					451:455	the regenerated cellulose beads	425:455	the regenerated cellulose beads mixed with maghemite nanoparticles	425:490	Sodium periodate oxidation process was carried out to form dialdehyde functional groups on the regenerated cellulose beads mixed with maghemite nanoparticles.
31730988	5	35	theme	X-ray	738:742	arg1	EDS					758:760	EDS	758:760	EDS	758:760	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	35	theme	X-ray	738:742	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	35	theme	X-ray	738:742	arg1	spectroscopy					744:755	energy dispersive X-ray spectroscopy	720:755	energy dispersive X-ray spectroscopy (EDS)	720:761	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	8	36	theme	safety	1268:1273	arg1	management					1275:1284	food safety management	1263:1284	food safety management	1263:1284	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	8	37	theme	grafted	1206:1212	arg1	beads					1224:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads	1183:1228	The biodegradable HTCC grafted cellulose beads may provide a novel approach for food safety management.
31730988	5	38	theme	X-ray	764:768	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	38	theme	X-ray	764:768	arg1	XRD					783:785	XRD	783:785	XRD	783:785	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	38	theme	X-ray	764:768	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction (XRD)	764:786	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	0	39	theme	chitosan	57:64	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antibacterial effect of quaternized chitosan anchored cellulose beads.
31730988	0	39	theme	chitosan	57:64	arg1	effect					35:40	antibacterial effect	21:40	antibacterial effect	21:40	Characterization and antibacterial effect of quaternized chitosan anchored cellulose beads.
31730988	6	40	theme	thermal	927:933	arg1	gravity					935:941	differential thermal gravity	914:941	differential thermal gravity (DTG)	914:947	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	6	40	theme	thermal	927:933	arg1	DTG					944:946	DTG	944:946	DTG	944:946	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	7	41	theme	long-term	1023:1031	arg1	activities					1047:1056	A long-term antibacterial activities	1021:1056	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris	1021:1110	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads.
31730988	2	42	theme	chloride	230:237	arg1	HTCC					249:252	HTCC	249:252	HTCC	249:252	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	2	42	theme	chloride	230:237	arg1	chitosan					239:246	hydroxypropyl trimethyl ammonium chloride chitosan	197:246	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	2	43	theme	chitosan	239:246	arg1	beads					282:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	1	44	theme	great	137:141	arg1	threat					143:148	great threat	137:148	great threat to beverage industry	137:169	Some thermoduric food spoilage bacteria pose great threat to beverage industry.
31730988	6	45	theme	differential	914:925	arg1	gravity					935:941	differential thermal gravity	914:941	differential thermal gravity (DTG)	914:947	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	6	45	theme	differential	914:925	arg1	DTG					944:946	DTG	944:946	DTG	944:946	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	2	46	theme	ammonium	221:228	arg1	chloride					230:237	hydroxypropyl trimethyl ammonium chloride	197:237	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	7	47	theme	covalent	1141:1148	arg1	bond					1150:1153	the covalent bond	1137:1153	the covalent bond between HTCC and the beads	1137:1180	A long-term antibacterial activities of the beads against Alicyclobacillus acidoterrestris were confirmed caused by the covalent bond between HTCC and the beads.
31730988	5	48	theme	anchored	591:598	arg1	beads					600:604	HTCC anchored beads	586:604	HTCC anchored beads	586:604	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	6	49	theme	Thermal	829:835	arg1	stability					837:845	Thermal stability	829:845	Thermal stability of the beads	829:858	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	1	50	theme	spoilage	114:121	arg1	bacteria					123:130	Some thermoduric food spoilage bacteria	92:130	Some thermoduric food spoilage bacteria	92:130	Some thermoduric food spoilage bacteria pose great threat to beverage industry.
31730988	2	51	theme	trimethyl	211:219	arg1	chloride					230:237	hydroxypropyl trimethyl ammonium chloride	197:237	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	2	52	theme	cellulose	272:280	arg1	beads					282:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	5	53	dep	Fourier	624:630	arg1	transform					632:640	transform	632:640	transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM)	632:826	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	2	54	theme	hydroxypropyl	197:209	arg1	chloride					230:237	hydroxypropyl trimethyl ammonium chloride	197:237	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	2	55	theme	magnetic	263:270	arg1	beads					282:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	1	56	theme	beverage	153:160	arg1	industry					162:169	beverage industry	153:169	beverage industry	153:169	Some thermoduric food spoilage bacteria pose great threat to beverage industry.
31730988	5	57	dep	transform	632:640	arg1	infrared					642:649	infrared	642:649	transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM)	632:826	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	6	58	theme	beads	991:995	arg1	temperature					972:982	the decomposition temperature	954:982	the decomposition temperature of the beads	954:995	Thermal stability of the beads was estimated by thermogravimetric analysis (TGA) and differential thermal gravity (DTG), and the decomposition temperature of the beads were around 200-300 °C.
31730988	0	59	theme	cellulose	75:83	arg1	beads					85:89	cellulose beads	75:89	cellulose beads	75:89	Characterization and antibacterial effect of quaternized chitosan anchored cellulose beads.
31730988	4	60	theme	Schiff	532:537	arg1	reaction					544:551	Schiff base reaction	532:551	Schiff base reaction	532:551	HTCC was anchored on the beads through Schiff base reaction.
31730988	2	61	theme	grafted	255:261	arg1	beads					282:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads	197:286	To tackle the challenge, hydroxypropyl trimethyl ammonium chloride chitosan (HTCC) grafted magnetic cellulose beads have been prepared via a dropping technology.
31730988	5	62	theme	sample	802:807	arg1	spectra					658:664	(FTIR) spectra	651:664	(FTIR) spectra	651:664	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	62	theme	sample	802:807	arg1	magnetometer					809:820	vibrating sample magnetometer	792:820	vibrating sample magnetometer (VSM)	792:826	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
31730988	5	62	theme	sample	802:807	arg1	VSM					823:825	VSM	823:825	VSM	823:825	The structure and properties of HTCC anchored beads were evaluated by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM).
30813291	1	0	theme	economy	141:147	arg1	policy					149:154	The circular economy policy	128:154	The circular economy policy	128:154	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	2	1	theme	fossil	493:498	arg1	sources					500:506	fossil sources	493:506	fossil sources	493:506	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	3	2	from	coupling	513:520	arg1	order					560:564	order	560:564	order to lower costs and promote degradability	560:605	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	1	3	theme	bio-composites	239:252	arg1	market					254:259	the bio-composites market	235:259	the bio-composites market	235:259	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	6	4	theme	impact	1294:1299	arg1	test					1301:1304	impact test	1294:1304	impact test	1294:1304	The mechanical properties were evaluated (tensile and impact test), and analytical models were applied in order to estimate the adhesion between matrix and fibers and to predict the material's stiffness.
30813291	2	5	attach	derived	353:359	arg1	waste					397:401	industrial and/or agricultural waste	366:401	industrial and/or agricultural waste	366:401	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	2	5	attach	derived	353:359	arg2	opportunity					266:276	The opportunity	262:276	The opportunity	262:276	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	3	6	with	bioplastics	525:535	arg1	fibers					550:555	natural fibers	542:555	natural fibers	542:555	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	0	7	with	Poly	79:82	arg1	Fibers					120:125	Natural Cellulose Fibers	102:125	Natural Cellulose Fibers	102:125	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	1	8	theme	market	254:259	arg1	expansion					222:230	a constant expansion	211:230	a constant expansion of the bio-composites market	211:259	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	0	9	theme	Lactic	84:89	arg1	Poly					79:82	Poly	79:82	Poly(Lactic Acid) with Natural Cellulose Fibers	79:125	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	0	9	theme	Lactic	84:89	arg1	Acid					91:94	Lactic Acid	84:94	Lactic Acid	84:94	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	3	10	dep	costs	575:579	arg1	lower					569:573	lower	569:573	lower	569:573	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	7	11	theme	particular	1481:1490	arg1	importance					1492:1501	particular importance	1481:1501	particular importance	1481:1501	Understanding these properties is of particular importance in order to be able to tune and project the final characteristics of bio-composites.
30813291	3	12	dep	packaging	670:678	arg1	the					666:668	the	666:668	the	666:668	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	4	13	theme	compostable	929:939	arg1	compostable					929:939	the most used compostable	915:939	the most used compostable	915:939	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	13	theme	compostable	929:939	arg1	one					908:910	one	908:910	one	908:910	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	13	theme	compostable	929:939	arg1	matrices					962:969	bio-based polymeric matrices	942:969	bio-based polymeric matrices	942:969	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	3	14	theme	non-recyclable	728:741	arg1	plastics					743:750	non-recyclable plastics	728:750	non-recyclable plastics	728:750	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	15	theme	natural	542:548	arg1	fibers					550:555	natural fibers	542:555	natural fibers	542:555	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	16	theme	potential	819:827	arg1	accumulation					829:840	potential accumulation	819:840	potential accumulation in the environment	819:859	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	4	17	theme	used	924:927	arg1	compostable					929:939	the most used compostable	915:939	the most used compostable	915:939	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	17	theme	used	924:927	arg1	matrices					962:969	bio-based polymeric matrices	942:969	bio-based polymeric matrices	942:969	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	3	18	theme	large	711:715	arg1	amounts					717:723	large amounts	711:723	large amounts of non-recyclable plastics	711:750	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	18	theme	large	711:715	arg1	plastics					743:750	non-recyclable plastics	728:750	non-recyclable plastics	728:750	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	4	19	theme	polymeric	952:960	arg1	compostable					929:939	the most used compostable	915:939	the most used compostable	915:939	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	19	theme	polymeric	952:960	arg1	matrices					962:969	bio-based polymeric matrices	942:969	bio-based polymeric matrices	942:969	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	20	with	compatible	1032:1041	arg1	range					1055:1059	a wide range	1048:1059	a wide range of applications	1048:1075	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	6	21	dep	evaluated	1271:1279	arg1	tensile					1282:1288	tensile	1282:1288	tensile	1282:1288	The mechanical properties were evaluated (tensile and impact test), and analytical models were applied in order to estimate the adhesion between matrix and fibers and to predict the material's stiffness.
30813291	6	21	dep	evaluated	1271:1279	arg1	test					1301:1304	impact test	1294:1304	impact test	1294:1304	The mechanical properties were evaluated (tensile and impact test), and analytical models were applied in order to estimate the adhesion between matrix and fibers and to predict the material's stiffness.
30813291	2	22	theme	bio-based	305:313	arg1	matrices					343:350	bio-based and biodegradable polymeric matrices	305:350	bio-based and biodegradable polymeric matrices	305:350	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	3	23	theme	bioplastics	525:535	arg1	one					610:612	one	610:612	one	610:612	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	23	theme	bioplastics	525:535	arg1	coupling					513:520	The coupling	509:520	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability	509:605	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	23	theme	bioplastics	525:535	arg1	objectives					629:638	the primary objectives	617:638	the primary objectives of research	617:650	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	2	24	theme	agricultural	384:395	arg1	waste					397:401	industrial and/or agricultural waste	366:401	industrial and/or agricultural waste	366:401	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	4	25	theme	process	990:996	arg1	ability					998:1004	process ability	990:1004	process ability	990:1004	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	7	26	theme	bio-composites	1572:1585	arg1	characteristics					1553:1567	the final characteristics	1543:1567	the final characteristics of bio-composites	1543:1585	Understanding these properties is of particular importance in order to be able to tune and project the final characteristics of bio-composites.
30813291	4	27	theme	mechanical	1010:1019	arg1	properties					1021:1030	mechanical properties	1010:1030	mechanical properties	1010:1030	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	7	28	theme	final	1547:1551	arg1	characteristics					1553:1567	the final characteristics	1543:1567	the final characteristics of bio-composites	1543:1585	Understanding these properties is of particular importance in order to be able to tune and project the final characteristics of bio-composites.
30813291	0	29	theme	Cellulose	110:118	arg1	Fibers					120:125	Natural Cellulose Fibers	102:125	Natural Cellulose Fibers	102:125	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	1	30	theme	sustainable	177:187	arg1	material					189:196	sustainable material	177:196	sustainable material	177:196	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	0	31	theme	Mechanical	14:23	arg1	Properties					41:50	Mechanical and Interfacial Properties	14:50	Properties	41:50	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	3	32	theme	plastics	743:750	arg1	amounts					717:723	large amounts	711:723	large amounts of non-recyclable plastics	711:750	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	32	theme	plastics	743:750	arg1	plastics					743:750	non-recyclable plastics	728:750	non-recyclable plastics	728:750	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	0	33	theme	Natural	102:108	arg1	Fibers					120:125	Natural Cellulose Fibers	102:125	Natural Cellulose Fibers	102:125	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	3	34	from	accumulation	829:840	arg1	environment					849:859	the environment	845:859	the environment	845:859	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	2	35	theme	natural	287:293	arg1	fibers					295:300	natural fibers	287:300	natural fibers in bio-based and biodegradable polymeric matrices	287:350	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	2	36	theme	stimulating	417:427	arg1	challenge					429:437	a stimulating challenge	415:437	a stimulating challenge in the replacement of traditional composites based on fossil sources	415:506	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	0	37	theme	Properties	41:50	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Mechanical and Interfacial Properties of Bio-Composites	0:68	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	5	38	dep	microfibers	1212:1222	arg1	%					1226:1226	5%	1225:1226	5%	1225:1226	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	5	38	dep	microfibers	1212:1222	arg1	%					1231:1231	10%	1229:1231	10%	1229:1231	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	5	38	dep	microfibers	1212:1222	arg1	%					1236:1236	20%	1234:1236	20%	1234:1236	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	0	39	theme	Interfacial	29:39	arg1	Properties					41:50	Mechanical and Interfacial Properties	14:50	Properties	41:50	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	3	40	theme	serious	778:784	arg1	problem					786:792	a serious problem	776:792	a serious problem for plastic disposal and potential accumulation in the environment	776:859	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	6	41	theme	mechanical	1244:1253	arg1	properties					1255:1264	The mechanical properties	1240:1264	The mechanical properties	1240:1264	The mechanical properties were evaluated (tensile and impact test), and analytical models were applied in order to estimate the adhesion between matrix and fibers and to predict the material's stiffness.
30813291	2	42	theme	composites	473:482	arg1	replacement					446:456	the replacement	442:456	the replacement of traditional composites based on fossil sources	442:506	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	5	43	theme	fibers	1117:1122	arg1	types					1097:1101	two types	1093:1101	two types of cellulosic fibers	1093:1122	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	4	44	theme	lactic	886:891	arg1	poly					881:884	poly	881:884	poly(lactic acid) (PLA)	881:903	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	44	theme	lactic	886:891	arg1	acid					893:896	lactic acid	886:896	lactic acid	886:896	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	3	45	theme	research	643:650	arg1	objectives					629:638	the primary objectives	617:638	the primary objectives of research	617:650	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	2	46	theme	traditional	461:471	arg1	composites					473:482	traditional composites	461:482	traditional composites based on fossil sources	461:506	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	5	47	theme	cellulosic	1106:1115	arg1	fibers					1117:1122	cellulosic fibers	1106:1122	cellulosic fibers	1106:1122	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	2	48	theme	polymeric	333:341	arg1	matrices					343:350	bio-based and biodegradable polymeric matrices	305:350	bio-based and biodegradable polymeric matrices	305:350	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	3	49	from	disposal	806:813	arg1	environment					849:859	the environment	845:859	the environment	845:859	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	50	theme	primary	621:627	arg1	objectives					629:638	the primary objectives	617:638	the primary objectives of research	617:650	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	2	51	theme	biodegradable	319:331	arg1	matrices					343:350	bio-based and biodegradable polymeric matrices	305:350	bio-based and biodegradable polymeric matrices	305:350	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	4	52	theme	wide	1050:1053	arg1	range					1055:1059	a wide range	1048:1059	a wide range of applications	1048:1075	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	5	53	theme	different	1187:1195	arg1	percentages					1197:1207	different percentages	1187:1207	different percentages of microfibers (5%, 10%, 20%)	1187:1237	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	3	54	theme	objectives	629:638	arg1	one					610:612	one	610:612	one	610:612	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	54	theme	objectives	629:638	arg1	coupling					513:520	The coupling	509:520	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability	509:605	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	54	theme	objectives	629:638	arg1	objectives					629:638	the primary objectives	617:638	the primary objectives of research	617:650	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	55	dep	order	560:564	arg1	promote					585:591	promote	585:591	promote degradability	585:605	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	3	55	dep	order	560:564	arg1	costs					575:579	costs	575:579	costs	575:579	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	4	56	theme	applications	1064:1075	arg1	range					1055:1059	a wide range	1048:1059	a wide range of applications	1048:1075	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	1	57	theme	constant	213:220	arg1	expansion					222:230	a constant expansion	211:230	a constant expansion of the bio-composites market	211:259	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	4	58	theme	bio-based	942:950	arg1	compostable					929:939	the most used compostable	915:939	the most used compostable	915:939	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	4	58	theme	bio-based	942:950	arg1	matrices					962:969	bio-based polymeric matrices	942:969	bio-based polymeric matrices	942:969	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	3	59	theme	agricultural	684:695	arg1	sectors					697:703	agricultural sectors	684:703	agricultural sectors	684:703	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30813291	4	60	theme	compatible	1032:1041	arg1	ability					998:1004	process ability	990:1004	process ability	990:1004	Among biopolymers, poly(lactic acid) (PLA) is one of the most used compostable, bio-based polymeric matrices, since it exhibits process ability and mechanical properties compatible with a wide range of applications.
30813291	2	61	theme	industrial	366:375	arg1	waste					397:401	industrial and/or agricultural waste	366:401	industrial and/or agricultural waste	366:401	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	6	62	theme	analytical	1312:1321	arg1	models					1323:1328	analytical models	1312:1328	analytical models	1312:1328	The mechanical properties were evaluated (tensile and impact test), and analytical models were applied in order to estimate the adhesion between matrix and fibers and to predict the material's stiffness.
30813291	0	63	theme	Bio-Composites	55:68	arg1	Properties					41:50	Mechanical and Interfacial Properties	14:50	Properties	41:50	Evaluation of Mechanical and Interfacial Properties of Bio-Composites Based on Poly(Lactic Acid) with Natural Cellulose Fibers.
30813291	5	64	theme	microfibers	1212:1222	arg1	percentages					1197:1207	different percentages	1187:1207	different percentages of microfibers (5%, 10%, 20%)	1187:1237	In this study, two types of cellulosic fibers were processed with PLA in order to obtain bio-composites with different percentages of microfibers (5%, 10%, 20%).
30813291	2	65	from	challenge	429:437	arg1	replacement					446:456	the replacement	442:456	the replacement of traditional composites based on fossil sources	442:506	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	1	66	theme	circular	132:139	arg1	policy					149:154	The circular economy policy	128:154	The circular economy policy	128:154	The circular economy policy and the interest for sustainable material are inducing a constant expansion of the bio-composites market.
30813291	2	67	from	fibers	295:300	arg1	matrices					343:350	bio-based and biodegradable polymeric matrices	305:350	bio-based and biodegradable polymeric matrices	305:350	The opportunity of using natural fibers in bio-based and biodegradable polymeric matrices, derived from industrial and/or agricultural waste, represents a stimulating challenge in the replacement of traditional composites based on fossil sources.
30813291	3	68	theme	plastic	798:804	arg1	disposal					806:813	plastic disposal	798:813	plastic disposal	798:813	The coupling of bioplastics with natural fibers in order to lower costs and promote degradability is one of the primary objectives of research, above all in the packaging and agricultural sectors where large amounts of non-recyclable plastics are generated, inducing a serious problem for plastic disposal and potential accumulation in the environment.
30173388	14	0	theme	reducing	1312:1319	arg1	sugars/g					1321:1328	reducing sugars/g	1312:1328	reducing sugars/g of wheat straw	1312:1343	The simultaneous system converted 368 mg of reducing sugars/g of wheat straw.
30173388	4	1	theme	BMP01	409:413	arg1	strains					415:421	BMP01 strains	409:421	BMP01 strains	409:421	BMP01 strains, were isolated and identified from wood-feeding termite's guts.
30173388	16	2	theme	Scanning	1516:1523	arg1	microscope					1534:1543	Scanning electron microscope	1516:1543	Scanning electron microscope	1516:1543	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	8	3	theme	oryzae	728:733	arg1	sp					750:751	Ochrobactrum oryzae BMP03-Bacillus sp	715:751	Ochrobactrum oryzae BMP03-Bacillus sp.	715:752	Ochrobactrum oryzae BMP03-Bacillus sp.
30173388	12	4	theme	Ochrobactrum	1100:1111	arg1	strain					1126:1131	the Ochrobactrum oryzae BMP03 strain	1096:1131	the Ochrobactrum oryzae BMP03 strain	1096:1131	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	2	5	theme	lignocellulose	267:280	arg1	biomass					282:288	lignocellulose biomass	267:288	lignocellulose biomass	267:288	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	18	6	theme	biofuel	1880:1886	arg1	production					1888:1897	biofuel production	1880:1897	biofuel production from wheat straw	1880:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	1	7	theme	biofuel	102:108	arg1	production					110:119	biofuel production	102:119	biofuel production	102:119	BMP01 bacteria to enhance biofuel production by increasing total reducing sugars yield.
30173388	5	8	theme	Ochrobactrum	521:532	arg1	strains					556:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	17	9	theme	production	1789:1798	arg1	cost					1773:1776	the cost	1769:1776	the cost of biofuel production	1769:1798	The biotreatment improved hydrolysis efficiency, which reduces the cost of biofuel production and increases the yield of biofuel.
30173388	16	10	from	change	1663:1668	arg1	composition					1673:1683	composition	1673:1683	composition due to biotreatment	1673:1703	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	15	11	theme	efficiency	1459:1468	arg1	terms					1439:1443	terms	1439:1443	terms of hydrolysis efficiency	1439:1468	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	10	12	theme	total	876:880	arg1	sugars					891:896	total reducing sugars	876:896	total reducing sugars in a separate hydrolysis system	876:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	4	13	theme	wood-feeding	458:469	arg1	guts					481:484	wood-feeding termite's guts	458:484	wood-feeding termite's guts	458:484	BMP01 strains, were isolated and identified from wood-feeding termite's guts.
30173388	18	14	from	possibilities	1863:1875	arg1	straw					1910:1914	wheat straw	1904:1914	wheat straw	1904:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	15	theme	effective	294:302	arg1	sp					405:406	Bacillus sp	396:406	Bacillus sp	396:406	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	15	theme	effective	294:302	arg1	lignin-degrading					308:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	15	theme	effective	294:302	arg1	BMP03					386:390	Ochrobactrum oryzae BMP03	366:390	Ochrobactrum oryzae BMP03	366:390	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	13	16	theme	cellulose	1203:1211	arg1	content					1213:1219	cellulose content	1203:1219	cellulose content	1203:1219	This also contributed for increment in cellulose content by 22.38% and hemicellulose content by 18.64%.
30173388	11	17	theme	simultaneous	1039:1050	arg1	system					1052:1057	the simultaneous system	1035:1057	the simultaneous system	1035:1057	BMP01 strain achieved 439 mg/g at 16 days of hydrolysis time, which is 9.45% higher than the simultaneous system.
30173388	9	18	theme	simultaneous	819:830	arg1	system					832:837	the simultaneous system	815:837	the simultaneous system	815:837	BMP01 consortia were also performed to analyze the effect of the simultaneous system.
30173388	16	19	dep	transform	1579:1587	arg1	infrared					1589:1596	infrared	1589:1596	transform infrared spectroscopy (FTIR) and X-ray diffraction analysis	1579:1647	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	6	20	theme	Bacillus	687:694	arg1	sp					696:697	Bacillus sp	687:697	Bacillus sp	687:697	The biodelignified wheat straw was hydrolyzed by Bacillus sp.
30173388	5	21	theme	BMP03	541:545	arg1	strains					556:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	10	22	theme	sugars	891:896	arg1	production					862:871	the production	858:871	the production of total reducing sugars in a separate hydrolysis system by Bacillus sp	858:943	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	2	23	from	biomass	282:288	arg1	production					251:260	production	251:260	production from lignocellulose biomass	251:288	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	3	24	theme	polysaccharide-hydrolyzing	329:354	arg1	bacteria					356:363	polysaccharide-hydrolyzing bacteria	329:363	polysaccharide-hydrolyzing bacteria	329:363	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	18	25	from	straw	1910:1914	arg1	possibilities					1863:1875	the possibilities	1859:1875	the possibilities of biofuel production from wheat straw	1859:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	18	25	from	straw	1910:1914	arg1	production					1888:1897	biofuel production	1880:1897	biofuel production from wheat straw	1880:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	15	26	theme	time	1510:1513	arg1	terms					1488:1492	terms	1488:1492	terms of biotreatment time	1488:1513	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	12	27	dep	%	1071:1071	arg1	44.47					1066:1070	44.47	1066:1070	44.47	1066:1070	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	1	28	theme	reducing	141:148	arg1	yield					157:161	total reducing sugars yield	135:161	total reducing sugars yield	135:161	BMP01 bacteria to enhance biofuel production by increasing total reducing sugars yield.
30173388	17	29	theme	hydrolysis	1732:1741	arg1	efficiency					1743:1752	hydrolysis efficiency	1732:1752	hydrolysis efficiency	1732:1752	The biotreatment improved hydrolysis efficiency, which reduces the cost of biofuel production and increases the yield of biofuel.
30173388	16	30	theme	diffraction	1628:1638	arg1	analysis					1640:1647	X-ray diffraction analysis	1622:1647	X-ray diffraction analysis	1622:1647	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	10	31	theme	hydrolysis	912:921	arg1	system					923:928	a separate hydrolysis system	901:928	a separate hydrolysis system	901:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	6	32	theme	biodelignified	642:655	arg1	straw					663:667	The biodelignified wheat straw	638:667	The biodelignified wheat straw	638:667	The biodelignified wheat straw was hydrolyzed by Bacillus sp.
30173388	5	33	theme	bacteria	547:554	arg1	strains					556:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Ochrobactrum oryzae BMP03 bacteria strains	521:562	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	16	34	theme	mid-infrared	1546:1557	arg1	analysis					1559:1566	mid-infrared analysis	1546:1566	mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis	1546:1647	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	10	35	from	system	923:928	arg1	production					862:871	the production	858:871	the production of total reducing sugars in a separate hydrolysis system by Bacillus sp	858:943	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	11	36	theme	time	1002:1005	arg1	16 days					980:986	16 days	980:986	16 days	980:986	BMP01 strain achieved 439 mg/g at 16 days of hydrolysis time, which is 9.45% higher than the simultaneous system.
30173388	5	37	theme	trapped	601:607	arg1	cellulose					609:617	cellulose	609:617	cellulose	609:617	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	19	38	theme	BMP01	1972:1976	arg1	bacteria					1978:1985	BMP01 bacteria	1972:1985	BMP01 bacteria	1972:1985	BMP01 bacteria strains.
30173388	12	39	dep	lignin	1073:1078	arg1	%					1071:1071	%	1071:1071	%	1071:1071	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	18	40	dep	Ochrobactrum	1929:1940	arg1	oryzae					1942:1947	oryzae	1942:1947	oryzae	1942:1947	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	0	41	theme	wheat	18:22	arg1	straw					24:28	wheat straw	18:28	wheat straw	18:28	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	1	42	theme	total	135:139	arg1	yield					157:161	total reducing sugars yield	135:161	total reducing sugars yield	135:161	BMP01 bacteria to enhance biofuel production by increasing total reducing sugars yield.
30173388	14	43	theme	straw	1339:1343	arg1	sugars/g					1321:1328	reducing sugars/g	1312:1328	reducing sugars/g of wheat straw	1312:1343	The simultaneous system converted 368 mg of reducing sugars/g of wheat straw.
30173388	12	44	theme	BMP03	1120:1124	arg1	strain					1126:1131	the Ochrobactrum oryzae BMP03 strain	1096:1131	the Ochrobactrum oryzae BMP03 strain	1096:1131	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	14	45	theme	wheat	1333:1337	arg1	straw					1339:1343	wheat straw	1333:1343	wheat straw	1333:1343	The simultaneous system converted 368 mg of reducing sugars/g of wheat straw.
30173388	15	46	theme	simultaneous	1416:1427	arg1	system					1429:1434	the simultaneous system	1412:1434	the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time	1412:1513	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	2	47	theme	reducing	223:230	arg1	sugars					232:237	total reducing sugars	217:237	total reducing sugars	217:237	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	18	48	theme	Ochrobactrum	1929:1940	arg1	BMP03					1949:1953	Ochrobactrum oryzae BMP03	1929:1953	Ochrobactrum oryzae BMP03	1929:1953	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	2	49	theme	vital	182:186	arg1	step					188:191	a vital step	180:191	a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass	180:288	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	2	49	theme	vital	182:186	arg1	Pretreatment					164:175	Pretreatment	164:175	Pretreatment	164:175	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	8	50	theme	BMP03-Bacillus	735:748	arg1	sp					750:751	Ochrobactrum oryzae BMP03-Bacillus sp	715:751	Ochrobactrum oryzae BMP03-Bacillus sp.	715:752	Ochrobactrum oryzae BMP03-Bacillus sp.
30173388	18	51	theme	Bacillus	1959:1966	arg1	sp					1968:1969	Bacillus sp	1959:1969	Bacillus sp	1959:1969	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	16	52	theme	electron	1525:1532	arg1	microscope					1534:1543	Scanning electron microscope	1516:1543	Scanning electron microscope	1516:1543	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	16	53	dep	Fourier	1571:1577	arg1	transform					1579:1587	transform	1579:1587	transform infrared spectroscopy (FTIR) and X-ray diffraction analysis	1579:1647	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	9	54	theme	BMP01	754:758	arg1	consortia					760:768	BMP01 consortia	754:768	BMP01 consortia	754:768	BMP01 consortia were also performed to analyze the effect of the simultaneous system.
30173388	8	55	theme	Ochrobactrum	715:726	arg1	sp					750:751	Ochrobactrum oryzae BMP03-Bacillus sp	715:751	Ochrobactrum oryzae BMP03-Bacillus sp.	715:752	Ochrobactrum oryzae BMP03-Bacillus sp.
30173388	14	56	theme	simultaneous	1272:1283	arg1	system					1285:1290	The simultaneous system	1268:1290	The simultaneous system	1268:1290	The simultaneous system converted 368 mg of reducing sugars/g of wheat straw.
30173388	17	57	theme	biofuel	1781:1787	arg1	production					1789:1798	biofuel production	1781:1798	biofuel production	1781:1798	The biotreatment improved hydrolysis efficiency, which reduces the cost of biofuel production and increases the yield of biofuel.
30173388	13	58	theme	hemicellulose	1235:1247	arg1	content					1249:1255	hemicellulose content	1235:1255	hemicellulose content	1235:1255	This also contributed for increment in cellulose content by 22.38% and hemicellulose content by 18.64%.
30173388	15	59	from	system	1429:1434	arg1	terms					1488:1492	terms	1488:1492	terms of biotreatment time	1488:1513	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	15	59	from	system	1429:1434	arg1	terms					1439:1443	terms	1439:1443	terms of hydrolysis efficiency	1439:1468	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	5	60	theme	Wheat	487:491	arg1	straw					493:497	Wheat straw	487:497	Wheat straw	487:497	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	10	61	theme	reducing	882:889	arg1	sugars					891:896	total reducing sugars	876:896	total reducing sugars in a separate hydrolysis system	876:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	4	62	theme	termite	471:477	arg1	guts					481:484	wood-feeding termite's guts	458:484	wood-feeding termite's guts	458:484	BMP01 strains, were isolated and identified from wood-feeding termite's guts.
30173388	2	63	theme	total	217:221	arg1	sugars					232:237	total reducing sugars	217:237	total reducing sugars	217:237	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	18	64	theme	production	1888:1897	arg1	possibilities					1863:1875	the possibilities	1859:1875	the possibilities of biofuel production from wheat straw	1859:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	5	65	dep	Ochrobactrum	521:532	arg1	oryzae					534:539	oryzae	534:539	oryzae	534:539	Wheat straw was biodelignified by Ochrobactrum oryzae BMP03 bacteria strains to degrade lignin and to release the trapped cellulose and hemicellulose.
30173388	15	66	theme	hydrolysis	1448:1457	arg1	efficiency					1459:1468	hydrolysis efficiency	1448:1468	hydrolysis efficiency	1448:1468	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	10	67	theme	separate	903:910	arg1	system					923:928	a separate hydrolysis system	901:928	a separate hydrolysis system	901:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	3	68	theme	new	304:306	arg1	sp					405:406	Bacillus sp	396:406	Bacillus sp	396:406	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	68	theme	new	304:306	arg1	lignin-degrading					308:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	68	theme	new	304:306	arg1	BMP03					386:390	Ochrobactrum oryzae BMP03	366:390	Ochrobactrum oryzae BMP03	366:390	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	9	69	theme	system	832:837	arg1	effect					805:810	the effect	801:810	the effect of the simultaneous system	801:837	BMP01 consortia were also performed to analyze the effect of the simultaneous system.
30173388	16	70	theme	X-ray	1622:1626	arg1	analysis					1640:1647	X-ray diffraction analysis	1622:1647	X-ray diffraction analysis	1622:1647	Scanning electron microscope, mid-infrared analysis by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis confirmed the change in composition due to biotreatment.
30173388	18	71	theme	wheat	1904:1908	arg1	straw					1910:1914	wheat straw	1904:1914	wheat straw	1904:1914	These results indicate the possibilities of biofuel production from wheat straw by employing Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	0	72	theme	Bacillus	63:70	arg1	sp					72:73	Bacillus sp	63:73	Bacillus sp	63:73	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	2	73	theme	sugars	232:237	arg1	yield					208:212	the yield	204:212	the yield of total reducing sugars	204:237	Pretreatment is a vital step to enhance the yield of total reducing sugars and biofuel production from lignocellulose biomass.
30173388	10	74	from	production	862:871	arg1	system					923:928	a separate hydrolysis system	901:928	a separate hydrolysis system	901:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	10	75	theme	Bacillus	933:940	arg1	sp					942:943	Bacillus sp	933:943	Bacillus sp	933:943	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	15	76	contain	have	1389:1392	arg2	advantage					1397:1405	an advantage	1394:1405	an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time	1394:1513	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	15	76	contain	have	1389:1392	arg1	hydrolysis					1378:1387	hydrolysis	1378:1387	hydrolysis	1378:1387	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	15	76	contain	have	1389:1392	arg1	biodelignification					1355:1372	Separate biodelignification	1346:1372	Separate biodelignification	1346:1372	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	1	77	theme	sugars	150:155	arg1	yield					157:161	total reducing sugars yield	135:161	total reducing sugars yield	135:161	BMP01 bacteria to enhance biofuel production by increasing total reducing sugars yield.
30173388	6	78	theme	wheat	657:661	arg1	straw					663:667	The biodelignified wheat straw	638:667	The biodelignified wheat straw	638:667	The biodelignified wheat straw was hydrolyzed by Bacillus sp.
30173388	15	79	theme	biotreatment	1497:1508	arg1	time					1510:1513	biotreatment time	1497:1513	biotreatment time	1497:1513	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	10	80	from	sugars	891:896	arg1	system					923:928	a separate hydrolysis system	901:928	a separate hydrolysis system	901:928	It was shown that the production of total reducing sugars in a separate hydrolysis system by Bacillus sp.
30173388	3	81	theme	Ochrobactrum	366:377	arg1	lignin-degrading					308:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	81	theme	Ochrobactrum	366:377	arg1	BMP03					386:390	Ochrobactrum oryzae BMP03	366:390	Ochrobactrum oryzae BMP03	366:390	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	13	82	from	increment	1190:1198	arg1	content					1213:1219	cellulose content	1203:1219	cellulose content	1203:1219	This also contributed for increment in cellulose content by 22.38% and hemicellulose content by 18.64%.
30173388	12	83	theme	biotreatment	1150:1161	arg1	16 days					1139:1145	16 days	1139:1145	16 days of biotreatment	1139:1161	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	1	84	theme	BMP01	76:80	arg1	bacteria					82:89	BMP01 bacteria	76:89	BMP01 bacteria	76:89	BMP01 bacteria to enhance biofuel production by increasing total reducing sugars yield.
30173388	15	85	theme	Separate	1346:1353	arg1	biodelignification					1355:1372	Separate biodelignification	1346:1372	Separate biodelignification	1346:1372	Separate biodelignification and hydrolysis have an advantage over the simultaneous system in terms of hydrolysis efficiency and vice versa in terms of biotreatment time.
30173388	0	86	theme	straw	24:28	arg1	Biodegradation					0:13	Biodegradation	0:13	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.	0:74	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	87	theme	Bacillus	396:403	arg1	sp					405:406	Bacillus sp	396:406	Bacillus sp	396:406	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	3	87	theme	Bacillus	396:403	arg1	lignin-degrading					308:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading	291:323	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	0	88	theme	Ochrobactrum	33:44	arg1	BMP03					53:57	Ochrobactrum oryzae BMP03	33:57	Ochrobactrum oryzae BMP03	33:57	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	11	89	theme	hydrolysis	991:1000	arg1	time					1002:1005	hydrolysis time	991:1005	hydrolysis time	991:1005	BMP01 strain achieved 439 mg/g at 16 days of hydrolysis time, which is 9.45% higher than the simultaneous system.
30173388	12	90	dep	Ochrobactrum	1100:1111	arg1	oryzae					1113:1118	oryzae	1113:1118	oryzae	1113:1118	About 44.47% lignin was degraded by the Ochrobactrum oryzae BMP03 strain after 16 days of biotreatment.
30173388	0	91	dep	Ochrobactrum	33:44	arg1	oryzae					46:51	oryzae	46:51	oryzae	46:51	Biodegradation of wheat straw by Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	14	92	theme	sugars/g	1321:1328	arg1	368 mg					1302:1307	368 mg	1302:1307	368 mg of reducing sugars/g of wheat straw	1302:1343	The simultaneous system converted 368 mg of reducing sugars/g of wheat straw.
30173388	3	93	dep	Ochrobactrum	366:377	arg1	oryzae					379:384	oryzae	379:384	oryzae	379:384	An effective new lignin-degrading and polysaccharide-hydrolyzing bacteria, Ochrobactrum oryzae BMP03 and Bacillus sp.
30173388	17	94	theme	biofuel	1827:1833	arg1	yield					1818:1822	the yield	1814:1822	the yield of biofuel	1814:1833	The biotreatment improved hydrolysis efficiency, which reduces the cost of biofuel production and increases the yield of biofuel.
31835712	0	0	theme	Gene	68:71	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	0	0	theme	Gene	68:71	arg1	Characterization					20:35	Partial Characterization	12:35	Partial Characterization	12:35	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	5	1	theme	endo-α-	991:997	arg1	gene					1016:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	4	2	theme	sequence	923:930	arg1	knowledge					892:900	knowledge	892:900	knowledge of its complete gene sequence	892:930	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	1	3	theme	biological	208:217	arg1	activities					219:228	biological activities	208:228	biological activities	208:228	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	0	4	theme	-d-Mannanase	55:66	arg1	Gene					68:71	an Endo-α-(1→6)-d-Mannanase Gene	40:71	an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans	40:95	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	6	5	theme	LM	1305:1306	arg1	precursors					1348:1357	LM and phosphatidyl-myo-inositol mannoside precursors	1305:1357	synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1264:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	1	6	theme	activities	219:228	arg1	array					199:203	broad array	193:203	broad array of biological activities	193:228	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	0	7	from	Cloning	0:6	arg1	circulans					87:95	Bacillus circulans	78:95	Bacillus circulans	78:95	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	4	8	theme	enzyme	794:799	arg1	form					781:784	no easily accessible form	760:784	no easily accessible form of this enzyme	760:799	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	3	9	theme	1→6	526:528	arg1	-D-mannanase					530:541	an endo-α-(1→6)-D-mannanase	515:541	an endo-α-(1→6)-D-mannanase from Bacillus circulans	515:565	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	5	10	from	cloning	967:973	arg1	TN-31					1045:1049	TN-31	1045:1049	TN-31	1045:1049	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	6	11	dep	production	1115:1124	arg1	the					1100:1102	the	1100:1102	the	1100:1102	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	5	12	theme	successful	956:965	arg1	cloning					967:973	the successful cloning	952:973	the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn	952:1076	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	6	13	theme	mannoside	1274:1282	arg1	acceptors					1284:1292	synthetic mannoside acceptors	1264:1292	synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1264:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	3	14	theme	Manp-	693:697	arg1	-Ino					716:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	3	14	theme	Manp-	693:697	arg1	end					650:652	the reducing end	637:652	the reducing end of these molecules	637:671	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	5	15	from	TN-31	1045:1049	arg1	gene					1016:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	5	15	from	TN-31	1045:1049	arg1	cloning					967:973	the successful cloning	952:973	the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn	952:1076	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	3	16	theme	endo-α-	518:524	arg1	-D-mannanase					530:541	an endo-α-(1→6)-D-mannanase	515:541	an endo-α-(1→6)-D-mannanase from Bacillus circulans	515:565	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	2	17	theme	internal	348:355	arg1	modifications					379:391	internal and terminal covalent modifications	348:391	internal and terminal covalent modifications	348:391	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	6	18	theme	substrate	1236:1244	arg1	specificity					1246:1256	its substrate specificity	1232:1256	its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1232:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	5	19	theme	complete	982:989	arg1	gene					1016:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	6	20	theme	synthetic	1264:1272	arg1	acceptors					1284:1292	synthetic mannoside acceptors	1264:1292	synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1264:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	2	21	theme	branching	414:422	arg1	patterns					424:431	complex internal branching patterns	397:431	complex internal branching patterns	397:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	6	22	theme	further	1211:1217	arg1	insight					1219:1225	further insight	1211:1225	further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1211:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	4	23	theme	accessible	770:779	arg1	form					781:784	no easily accessible form	760:784	no easily accessible form of this enzyme	760:799	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	2	24	from	heterogeneity	303:315	arg1	terms					339:343	terms	339:343	terms of internal and terminal covalent modifications and complex internal branching patterns	339:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	6	25	theme	hydrolase	1159:1167	arg1	domain					1169:1174	the glycosyl hydrolase domain	1146:1174	the glycosyl hydrolase domain of this enzyme	1146:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	25	theme	hydrolase	1159:1167	arg1	enzyme					1184:1189	this enzyme	1179:1189	this enzyme	1179:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	2	26	theme	internal	405:412	arg1	patterns					424:431	complex internal branching patterns	397:431	complex internal branching patterns	397:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	6	27	theme	successful	1104:1113	arg1	production					1115:1124	successful production	1104:1124	successful production	1104:1124	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	28	theme	enzyme	1184:1189	arg1	domain					1169:1174	the glycosyl hydrolase domain	1146:1174	the glycosyl hydrolase domain of this enzyme	1146:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	28	theme	enzyme	1184:1189	arg1	enzyme					1184:1189	this enzyme	1179:1189	this enzyme	1179:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	2	29	theme	complex	397:403	arg1	patterns					424:431	complex internal branching patterns	397:431	complex internal branching patterns	397:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	3	30	theme	[Manp-	704:709	arg1	-Ino					716:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	3	30	theme	[Manp-	704:709	arg1	end					650:652	the reducing end	637:652	the reducing end of these molecules	637:671	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	2	31	theme	modifications	379:391	arg1	terms					339:343	terms	339:343	terms of internal and terminal covalent modifications and complex internal branching patterns	339:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	0	32	theme	Partial	12:18	arg1	Characterization					20:35	Partial Characterization	12:35	Partial Characterization	12:35	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	4	33	theme	research	822:829	arg1	community					831:839	the research community	818:839	the research community	818:839	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	4	33	theme	research	822:829	arg1	fact					844:847	a fact	842:847	a fact that may in part be explained by a lack of knowledge of its complete gene sequence	842:930	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	3	34	theme	LAM	623:625	arg1	backbone					604:611	the mannan backbone	593:611	the mannan backbone of LM and LAM	593:625	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	2	35	theme	covalent	370:377	arg1	modifications					379:391	internal and terminal covalent modifications	348:391	internal and terminal covalent modifications	348:391	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	5	36	theme	1→6	999:1001	arg1	gene					1016:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	1	37	theme	fine	257:260	arg1	details					262:268	the fine details	253:268	the fine details of their structure	253:287	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	2	38	gly	heterogeneity	303:315	arg1	molecules					326:334	these molecules	320:334	these molecules	320:334	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	6	39	theme	mannoside	1338:1346	arg1	precursors					1348:1357	LM and phosphatidyl-myo-inositol mannoside precursors	1305:1357	synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1264:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	2	40	theme	terminal	361:368	arg1	modifications					379:391	internal and terminal covalent modifications	348:391	internal and terminal covalent modifications	348:391	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	2	41	theme	significant	443:453	arg1	obstacles					455:463	significant obstacles	443:463	significant obstacles to their structural characterization	443:500	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	3	42	theme	LM	616:617	arg1	backbone					604:611	the mannan backbone	593:611	the mannan backbone of LM and LAM	593:625	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	4	43	theme	gene	918:921	arg1	sequence					923:930	its complete gene sequence	905:930	its complete gene sequence	905:930	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	4	44	theme	knowledge	892:900	arg1	lack					884:887	a lack	882:887	a lack of knowledge of its complete gene sequence	882:930	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	4	45	theme	complete	909:916	arg1	sequence					923:930	its complete gene sequence	905:930	its complete gene sequence	905:930	Although first reported 45 years ago, no easily accessible form of this enzyme was available to the research community, a fact that may in part be explained by a lack of knowledge of its complete gene sequence.
31835712	6	46	theme	domain	1169:1174	arg1	purification					1130:1141	purification	1130:1141	purification	1130:1141	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	46	theme	domain	1169:1174	arg1	use					1199:1201	its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1195:1384	its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1195:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	46	theme	domain	1169:1174	arg1	production					1115:1124	successful production	1104:1124	successful production	1104:1124	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	2	47	theme	patterns	424:431	arg1	terms					339:343	terms	339:343	terms of internal and terminal covalent modifications and complex internal branching patterns	339:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	5	48	theme	gene	1016:1019	arg1	cloning					967:973	the successful cloning	952:973	the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn	952:1076	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
31835712	3	49	theme	molecules	663:671	arg1	-Ino					716:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	3	49	theme	molecules	663:671	arg1	end					650:652	the reducing end	637:652	the reducing end of these molecules	637:671	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	3	50	theme	reducing	641:648	arg1	-Ino					716:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Manp-(1→6) [Manp-(1→2)]-Ino	693:719	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	3	50	theme	reducing	641:648	arg1	end					650:652	the reducing end	637:652	the reducing end of these molecules	637:671	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	1	51	theme	structure	279:287	arg1	details					262:268	the fine details	253:268	the fine details of their structure	253:287	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	6	52	theme	glycosyl	1150:1157	arg1	domain					1169:1174	the glycosyl hydrolase domain	1146:1174	the glycosyl hydrolase domain of this enzyme	1146:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	6	52	theme	glycosyl	1150:1157	arg1	enzyme					1184:1189	this enzyme	1179:1189	this enzyme	1179:1189	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	0	53	from	circulans	87:95	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	0	53	from	circulans	87:95	arg1	Gene					68:71	an Endo-α-(1→6)-d-Mannanase Gene	40:71	an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans	40:95	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	0	53	from	circulans	87:95	arg1	Characterization					20:35	Partial Characterization	12:35	Partial Characterization	12:35	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	1	54	theme	major	123:127	arg1	lipoarabinomannan					162:178	lipoarabinomannan	162:178	lipoarabinomannan (LAM)	162:184	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	1	54	theme	major	123:127	arg1	lipomannan					142:151	lipomannan	142:151	lipomannan (LM)	142:156	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	1	54	theme	major	123:127	arg1	lipoglycans					129:139	two major lipoglycans	119:139	two major lipoglycans	119:139	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	1	55	theme	broad	193:197	arg1	array					199:203	broad array	193:203	broad array of biological activities	193:228	Mycobacteria produce two major lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), whose broad array of biological activities are tightly related to the fine details of their structure.
31835712	3	56	dep	-Ino	716:719	arg1	1→2					711:713	1→2	711:713	1→2	711:713	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	2	57	theme	molecules	326:334	arg1	heterogeneity					303:315	the heterogeneity	299:315	the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns	299:431	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	3	58	from	circulans	557:565	arg1	-D-mannanase					530:541	an endo-α-(1→6)-D-mannanase	515:541	an endo-α-(1→6)-D-mannanase from Bacillus circulans	515:565	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	2	59	theme	structural	474:483	arg1	characterization					485:500	their structural characterization	468:500	their structural characterization	468:500	However, the heterogeneity of these molecules in terms of internal and terminal covalent modifications and complex internal branching patterns represent significant obstacles to their structural characterization.
31835712	0	60	from	Characterization	20:35	arg1	circulans					87:95	Bacillus circulans	78:95	Bacillus circulans	78:95	Cloning and Partial Characterization of an Endo-α-(1→6)-d-Mannanase Gene from Bacillus circulans.
31835712	3	61	theme	mannan	597:602	arg1	backbone					604:611	the mannan backbone	593:611	the mannan backbone of LM and LAM	593:625	Previously, an endo-α-(1→6)-D-mannanase from Bacillus circulans proved useful in cleaving the mannan backbone of LM and LAM, allowing the reducing end of these molecules to be identified as Manp-(1→6) [Manp-(1→2)]-Ino.
31835712	6	62	theme	phosphatidyl-myo-inositol	1312:1336	arg1	precursors					1348:1357	LM and phosphatidyl-myo-inositol mannoside precursors	1305:1357	synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria	1264:1384	We further report on the successful production and purification of the glycosyl hydrolase domain of this enzyme and its use to gain further insight into its substrate specificity using synthetic mannoside acceptors as well as LM and phosphatidyl-myo-inositol mannoside precursors purified from mycobacteria.
31835712	5	63	theme	-D-mannanase	1003:1014	arg1	gene					1016:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	the complete endo-α-(1→6)-D-mannanase gene	978:1019	Here, we report on the successful cloning of the complete endo-α-(1→6)-D-mannanase gene from Bacillus circulans TN-31, herein referred to as emn.
30674645	14	0	theme	butyrate	2589:2596	arg1	beneficial					2609:2617	beneficial	2609:2617	beneficial	2609:2617	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	14	0	theme	butyrate	2589:2596	arg1	synthesis					2551:2559	the microbial synthesis	2537:2559	the microbial synthesis	2537:2559	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	4	1	theme	genetic	851:857	arg1	loci					859:862	genetic loci	851:862	genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	851:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	12	2	theme	novel	2251:2255	arg1	preparations					2270:2281	two novel wood-derived preparations	2247:2281	two novel wood-derived preparations	2247:2281	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	2	theme	novel	2251:2255	arg1	AcAGX					2294:2298	AcAGX	2294:2298	AcAGX	2294:2298	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	2	theme	novel	2251:2255	arg1	AcGGM					2284:2288	AcGGM	2284:2288	AcGGM	2284:2288	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	4	3	theme	GGM	577:579	arg1	potential					553:561	the prebiotic potential	539:561	the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture	539:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	4	theme	profound	1980:1987	arg1	effect					1989:1994	a profound effect	1978:1994	a profound effect	1978:1994	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	5	from	feedstocks	1765:1774	arg1	AcAGX					1738:1742	AcAGX	1738:1742	AcAGX	1738:1742	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	5	from	feedstocks	1765:1774	arg1	AcGGM					1728:1732	AcGGM	1728:1732	AcGGM	1728:1732	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	6	contain	has	1974:1976	arg1	architecture					1930:1941	The architecture	1926:1941	The architecture of the gut bacterial ecosystem	1926:1972	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	6	contain	has	1974:1976	arg2	effect					1989:1994	a profound effect	1978:1994	a profound effect	1978:1994	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	13	7	theme	IX	2515:2516	arg1	spp					2518:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	2	8	theme	predominant	170:180	arg1	hemicelluloses					182:195	The predominant hemicelluloses	166:195	The predominant hemicelluloses in softwood and hardwood	166:220	The predominant hemicelluloses in softwood and hardwood are galactoglucomannan (GGM) and arabinoglucuronoxylan (AGX), respectively.
30674645	2	8	theme	predominant	170:180	arg1	galactoglucomannan					226:243	galactoglucomannan	226:243	galactoglucomannan (GGM)	226:249	The predominant hemicelluloses in softwood and hardwood are galactoglucomannan (GGM) and arabinoglucuronoxylan (AGX), respectively.
30674645	10	9	theme	bacteria	1826:1833	arg1	promotion					1802:1810	the promotion	1798:1810	the promotion of beneficial bacteria	1798:1833	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	10	dep	in	1657:1658	arg1	vivo					1660:1663	vivo	1660:1663	vivo	1660:1663	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	4	11	from	potential	553:561	arg1	culture					691:697	vitro In pure culture	677:697	vitro In pure culture	677:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	12	dep	physiology	2003:2012	arg1	the					1999:2001	the	1999:2001	the	1999:2001	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	16	13	theme	gut	2973:2975	arg1	health					2977:2982	gut health	2973:2982	gut health	2973:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	4	14	theme	lignocellulosic	647:661	arg1	feedstocks					663:672	Norwegian lignocellulosic feedstocks	637:672	Norwegian lignocellulosic feedstocks	637:672	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	15	15	theme	potential	2711:2719	arg1	properties					2731:2740	potential prebiotic properties	2711:2740	potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks	2711:2797	Thus, our results demonstrate potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks.
30674645	4	16	theme	xylo-oligosaccharides/xylans	926:953	arg1	utilization					872:882	the utilization	868:882	the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	868:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	8	17	theme	Other	1315:1319	arg1	groups					1331:1336	Other bacterial groups	1315:1336	Other bacterial groups enumerated	1315:1347	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	10	18	theme	in	1657:1658	arg1	confirmation					1665:1676	further in vivo confirmation	1649:1676	further in vivo confirmation	1649:1676	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	5	19	theme	human	1103:1107	arg1	feces					1109:1113	healthy adult human feces	1089:1113	healthy adult human feces	1089:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	12	20	link	wood-derived	2257:2268	arg1	preparations					2270:2281	two novel wood-derived preparations	2247:2281	two novel wood-derived preparations	2247:2281	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	20	link	wood-derived	2257:2268	arg1	AcAGX					2294:2298	AcAGX	2294:2298	AcAGX	2294:2298	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	20	link	wood-derived	2257:2268	arg1	AcGGM					2284:2288	AcGGM	2284:2288	AcGGM	2284:2288	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	11	21	theme	prebiotics	2133:2142	arg1	administration					2115:2128	administration	2115:2128	administration of prebiotics	2115:2142	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	8	22	theme	cluster	1507:1513	arg1	P < 0.05					1519:1526	P < 0.05	1519:1526	P < 0.05	1519:1526	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	8	22	theme	cluster	1507:1513	arg1	IX					1515:1516	clostridial cluster IX	1495:1516	clostridial cluster IX (P < 0.05)	1495:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	16	23	theme	dietary	2908:2914	arg1	supplementation					2916:2930	the dietary supplementation	2904:2930	the dietary supplementation of AcGGM and AcAGX to improve or restore gut health	2904:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	10	24	theme	prebiotic	1864:1872	arg1	ability					1874:1880	a promising prebiotic ability	1852:1880	a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host	1852:2039	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	9	25	theme	other	1546:1550	arg1	substrates					1552:1561	the other substrates	1542:1561	the other substrates	1542:1561	Compared to the other substrates, AcGGM promoted butyrogenic fermentation whereas AcAGX was more propiogenic.
30674645	4	26	theme	β-manno-oligosaccharides/β-mannans	887:920	arg1	utilization					872:882	the utilization	868:882	the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	868:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	5	27	theme	healthy	1089:1095	arg1	feces					1109:1113	healthy adult human feces	1089:1113	healthy adult human feces	1089:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	10	28	theme	gut	1904:1906	arg1	health.IMPORTANCE					1908:1924	gut health.IMPORTANCE	1904:1924	gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host	1904:2039	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	1	29	theme	hemicellulosic	134:147	arg1	polysaccharides					149:163	hemicellulosic polysaccharides	134:163	hemicellulosic polysaccharides	134:163	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	3	30	theme	common	337:342	arg1	fibers					352:357	common dietary fibers	337:357	common dietary fibers	337:357	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	4	31	theme	In	683:684	arg1	culture					691:697	vitro In pure culture	677:697	vitro In pure culture	677:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	14	32	theme	epithelial	2663:2672	arg1	cells					2674:2678	the microbial ecosystem and host colonic epithelial cells	2622:2678	cells	2674:2678	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	11	33	theme	gut	2060:2062	arg1	microbiota					2064:2073	the gut microbiota and the intestinal microenvironment	2056:2109	microbiota	2064:2073	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	12	34	theme	human	2314:2318	arg1	composition					2335:2345	human gut microbiota composition	2314:2345	human gut microbiota composition	2314:2345	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	15	35	theme	lignocellulosic	2772:2786	arg1	feedstocks					2788:2797	lignocellulosic feedstocks	2772:2797	lignocellulosic feedstocks	2772:2797	Thus, our results demonstrate potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks.
30674645	11	36	theme	intestinal	2083:2092	arg1	microenvironment					2094:2109	the gut microbiota and the intestinal microenvironment	2056:2109	microenvironment	2094:2109	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	1	37	theme	Woody	69:73	arg1	biomass					75:81	Woody biomass	69:81	Woody biomass	69:81	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	1	37	theme	Woody	69:73	arg1	source					124:129	a sustainable and virtually unlimited source	86:129	a sustainable and virtually unlimited source of hemicellulosic polysaccharides	86:163	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	14	38	theme	host	2650:2653	arg1	cells					2674:2678	the microbial ecosystem and host colonic epithelial cells	2622:2678	cells	2674:2678	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	12	39	theme	microbiota	2324:2333	arg1	composition					2335:2345	human gut microbiota composition	2314:2345	human gut microbiota composition	2314:2345	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	8	40	theme	members	1416:1422	arg1	growth					1406:1411	the growth	1402:1411	the growth of members of the Bacteroides-Prevotella group	1402:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	0	41	theme	Wood-Derived	0:11	arg1	Fibers					21:26	Wood-Derived Dietary Fibers	0:26	Wood-Derived Dietary Fibers	0:26	Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota.
30674645	8	42	from	exception	1374:1382	arg1	growth					1406:1411	the growth	1402:1411	the growth of members of the Bacteroides-Prevotella group	1402:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	1	43	theme	sustainable	88:98	arg1	biomass					75:81	Woody biomass	69:81	Woody biomass	69:81	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	1	43	theme	sustainable	88:98	arg1	source					124:129	a sustainable and virtually unlimited source	86:129	a sustainable and virtually unlimited source of hemicellulosic polysaccharides	86:163	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	12	44	theme	preparations	2270:2281	arg1	ability					2236:2242	the ability	2232:2242	the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity	2232:2358	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	10	45	theme	lignocellulosic	1749:1763	arg1	feedstocks					1765:1774	lignocellulosic feedstocks	1749:1774	lignocellulosic feedstocks	1749:1774	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	11	46	theme	microbiota	2064:2073	arg1	Modulation					2042:2051	Modulation	2042:2051	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics	2042:2142	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	5	47	theme	AcGGM	983:987	arg1	potential					970:978	The prebiotic potential	956:978	The prebiotic potential of AcGGM and AcAGX	956:997	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	8	48	theme	group	1454:1458	arg1	members					1416:1422	members	1416:1422	members of the Bacteroides-Prevotella group	1416:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	0	49	theme	Beneficial	36:45	arg1	Microbiota					57:66	Beneficial Human Gut Microbiota	36:66	Beneficial Human Gut Microbiota	36:66	Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota.
30674645	14	50	theme	microbial	2626:2634	arg1	ecosystem					2636:2644	the microbial ecosystem and host colonic epithelial cells	2622:2678	ecosystem	2636:2644	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	5	51	theme	culture	1045:1051	arg1	system					1066:1071	a pH-controlled batch culture fermentation system	1023:1071	a pH-controlled batch culture fermentation system inoculated with healthy adult human feces	1023:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	16	52	theme	intervention	2871:2882	arg1	strategies					2884:2893	intervention strategies	2871:2893	intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health	2871:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	3	53	theme	prebiotic	398:406	arg1	properties					408:417	prebiotic properties	398:417	prebiotic properties	398:417	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	8	54	theme	Faecalibacterium	1461:1476	arg1	prausnitzii					1478:1488	Faecalibacterium prausnitzii	1461:1488	Faecalibacterium prausnitzii	1461:1488	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	14	55	theme	microbial	2541:2549	arg1	beneficial					2609:2617	beneficial	2609:2617	beneficial	2609:2617	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	14	55	theme	microbial	2541:2549	arg1	synthesis					2551:2559	the microbial synthesis	2537:2559	the microbial synthesis	2537:2559	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	0	56	theme	Gut	53:55	arg1	Microbiota					57:66	Beneficial Human Gut Microbiota	36:66	Beneficial Human Gut Microbiota	36:66	Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota.
30674645	13	57	theme	cluster	2507:2513	arg1	IX					2515:2516	clostridial cluster IX	2495:2516	clostridial cluster IX	2495:2516	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	10	58	theme	ecosystem	1964:1972	arg1	architecture					1930:1941	The architecture	1926:1941	The architecture of the gut bacterial ecosystem	1926:1972	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	4	59	theme	species	799:805	arg1	growth					741:746	the growth	737:746	the growth of Bifidobacterium, Lactobacillus, and Bacteroides species	737:805	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	60	theme	gut	1950:1952	arg1	ecosystem					1964:1972	the gut bacterial ecosystem	1946:1972	the gut bacterial ecosystem	1946:1972	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	8	61	theme	clostridial	1495:1505	arg1	P < 0.05					1519:1526	P < 0.05	1519:1526	P < 0.05	1519:1526	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	8	61	theme	clostridial	1495:1505	arg1	IX					1515:1516	clostridial cluster IX	1495:1516	clostridial cluster IX (P < 0.05)	1495:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	8	62	from	increase	1390:1397	arg1	growth					1406:1411	the growth	1402:1411	the growth of members of the Bacteroides-Prevotella group	1402:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	2	63	from	hemicelluloses	182:195	arg1	softwood					200:207	softwood	200:207	softwood	200:207	The predominant hemicelluloses in softwood and hardwood are galactoglucomannan (GGM) and arabinoglucuronoxylan (AGX), respectively.
30674645	2	63	from	hemicelluloses	182:195	arg1	hardwood					213:220	hardwood	213:220	hardwood	213:220	The predominant hemicelluloses in softwood and hardwood are galactoglucomannan (GGM) and arabinoglucuronoxylan (AGX), respectively.
30674645	10	64	used	used	1783:1786	arg2	AcAGX					1738:1742	AcAGX	1738:1742	AcAGX	1738:1742	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	64	used	used	1783:1786	arg2	AcGGM					1728:1732	AcGGM	1728:1732	AcGGM	1728:1732	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	12	65	theme	wood-derived	2257:2268	arg1	preparations					2270:2281	two novel wood-derived preparations	2247:2281	two novel wood-derived preparations	2247:2281	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	65	theme	wood-derived	2257:2268	arg1	AcAGX					2294:2298	AcAGX	2294:2298	AcAGX	2294:2298	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	12	65	theme	wood-derived	2257:2268	arg1	AcGGM					2284:2288	AcGGM	2284:2288	AcGGM	2284:2288	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	3	66	theme	microbiota	499:508	arg1	modulation					477:486	specific modulation	468:486	specific modulation of the gut microbiota	468:508	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	4	67	theme	acetylated	566:575	arg1	AcGGM					582:586	AcGGM	582:586	AcGGM	582:586	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	4	67	theme	acetylated	566:575	arg1	GGM					577:579	acetylated GGM	566:579	acetylated GGM (AcGGM)	566:587	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	68	theme	further	1649:1655	arg1	confirmation					1665:1676	further in vivo confirmation	1649:1676	further in vivo confirmation	1649:1676	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	13	69	theme	clostridial	2495:2505	arg1	IX					2515:2516	clostridial cluster IX	2495:2516	clostridial cluster IX	2495:2516	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	14	70	theme	propionate	2573:2582	arg1	beneficial					2609:2617	beneficial	2609:2617	beneficial	2609:2617	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	14	70	theme	propionate	2573:2582	arg1	synthesis					2551:2559	the microbial synthesis	2537:2559	the microbial synthesis	2537:2559	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	4	71	theme	loci	859:862	arg1	presence					839:846	the presence	835:846	the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	835:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	16	72	theme	AcAGX	2945:2949	arg1	supplementation					2916:2930	the dietary supplementation	2904:2930	the dietary supplementation of AcGGM and AcAGX to improve or restore gut health	2904:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	10	73	theme	beneficial	1815:1824	arg1	bacteria					1826:1833	beneficial bacteria	1815:1833	beneficial bacteria	1815:1833	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	74	dep	health.IMPORTANCE	1908:1924	arg1	has					1974:1976	has	1974:1976	has a profound effect on the physiology and well-being of the host	1974:2039	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	14	75	theme	acetate	2564:2570	arg1	beneficial					2609:2617	beneficial	2609:2617	beneficial	2609:2617	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	14	75	theme	acetate	2564:2570	arg1	synthesis					2551:2559	the microbial synthesis	2537:2559	the microbial synthesis	2537:2559	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	4	76	theme	consistent	819:828	arg1	manner					812:817	a manner	810:817	a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	810:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	77	theme	promising	1854:1862	arg1	ability					1874:1880	a promising prebiotic ability	1852:1880	a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host	1852:2039	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	13	78	theme	Bifidobacterium	2434:2448	arg1	spp					2518:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	3	79	contain	have	393:396	arg2	properties					408:417	prebiotic properties	398:417	prebiotic properties	398:417	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	3	79	contain	have	393:396	arg1	AGX					368:370	AGX	368:370	AGX	368:370	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	3	79	contain	have	393:396	arg1	GGM					360:362	GGM	360:362	GGM	360:362	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	6	80	theme	FOS	1200:1202	arg1	mixture					1205:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	Results were compared with those obtained with a commercial fructo-oligosaccharide (FOS) mixture.
30674645	4	81	theme	Norwegian	637:645	arg1	feedstocks					663:672	Norwegian lignocellulosic feedstocks	637:672	Norwegian lignocellulosic feedstocks	637:672	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	10	82	theme	host	2036:2039	arg1	physiology					2003:2012	physiology	2003:2012	physiology	2003:2012	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	10	82	theme	host	2036:2039	arg1	well-being					2018:2027	well-being	2018:2027	well-being	2018:2027	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	11	83	theme	microenvironment	2094:2109	arg1	Modulation					2042:2051	Modulation	2042:2051	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics	2042:2142	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	15	84	theme	prebiotic	2721:2729	arg1	properties					2731:2740	potential prebiotic properties	2711:2740	potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks	2711:2797	Thus, our results demonstrate potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks.
30674645	11	85	theme	valuable	2157:2164	arg1	strategy					2166:2173	a valuable strategy	2155:2173	a valuable strategy to promote host health	2155:2196	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	8	86	theme	IX	1515:1516	arg1	exception					1374:1382	the exception	1370:1382	the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05)	1370:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	6	87	theme	fructo-oligosaccharide	1176:1197	arg1	mixture					1205:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	Results were compared with those obtained with a commercial fructo-oligosaccharide (FOS) mixture.
30674645	4	88	theme	AGX	611:613	arg1	potential					553:561	the prebiotic potential	539:561	the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture	539:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	8	89	from	prausnitzii	1478:1488	arg1	growth					1406:1411	the growth	1402:1411	the growth of members of the Bacteroides-Prevotella group	1402:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	13	90	theme	commensal	2407:2415	arg1	bacteria					2417:2424	commensal bacteria	2407:2424	commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2407:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	13	90	theme	commensal	2407:2415	arg1	spp					2518:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	8	91	theme	bacterial	1321:1329	arg1	groups					1331:1336	Other bacterial groups	1315:1336	Other bacterial groups enumerated	1315:1347	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	1	92	theme	unlimited	114:122	arg1	biomass					75:81	Woody biomass	69:81	Woody biomass	69:81	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	1	92	theme	unlimited	114:122	arg1	source					124:129	a sustainable and virtually unlimited source	86:129	a sustainable and virtually unlimited source of hemicellulosic polysaccharides	86:163	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	11	93	theme	host	2186:2189	arg1	health					2191:2196	host health	2186:2196	host health	2186:2196	Modulation of the gut microbiota and the intestinal microenvironment via administration of prebiotics represents a valuable strategy to promote host health.
30674645	15	94	from	feedstocks	2788:2797	arg1	AcAGX					2761:2765	AcAGX	2761:2765	AcAGX	2761:2765	Thus, our results demonstrate potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks.
30674645	15	94	from	feedstocks	2788:2797	arg1	AcGGM					2751:2755	AcGGM	2751:2755	AcGGM	2751:2755	Thus, our results demonstrate potential prebiotic properties for both AcGGM and AcAGX from lignocellulosic feedstocks.
30674645	4	95	theme	pure	686:689	arg1	culture					691:697	vitro In pure culture	677:697	vitro In pure culture	677:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	3	96	theme	structure	311:319	arg1	similarity					321:330	the structure similarity	307:330	the structure similarity with common dietary fibers	307:357	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	5	97	theme	adult	1097:1101	arg1	feces					1109:1113	healthy adult human feces	1089:1113	healthy adult human feces	1089:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	1	98	theme	polysaccharides	149:163	arg1	biomass					75:81	Woody biomass	69:81	Woody biomass	69:81	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	1	98	theme	polysaccharides	149:163	arg1	source					124:129	a sustainable and virtually unlimited source	86:129	a sustainable and virtually unlimited source of hemicellulosic polysaccharides	86:163	Woody biomass is a sustainable and virtually unlimited source of hemicellulosic polysaccharides.
30674645	16	99	theme	AcGGM	2935:2939	arg1	supplementation					2916:2930	the dietary supplementation	2904:2930	the dietary supplementation of AcGGM and AcAGX to improve or restore gut health	2904:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	8	100	from	IX	1515:1516	arg1	growth					1406:1411	the growth	1402:1411	the growth of members of the Bacteroides-Prevotella group	1402:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	9	101	theme	butyrogenic	1579:1589	arg1	fermentation					1591:1602	butyrogenic fermentation	1579:1602	butyrogenic fermentation	1579:1602	Compared to the other substrates, AcGGM promoted butyrogenic fermentation whereas AcAGX was more propiogenic.
30674645	3	102	theme	dietary	344:350	arg1	fibers					352:357	common dietary fibers	337:357	common dietary fibers	337:357	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	4	103	theme	vitro	677:681	arg1	culture					691:697	vitro In pure culture	677:697	vitro In pure culture	677:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	4	104	theme	Lactobacillus	768:780	arg1	growth					741:746	the growth	737:746	the growth of Bifidobacterium, Lactobacillus, and Bacteroides species	737:805	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	4	105	with	consistent	819:828	arg1	presence					839:846	the presence	835:846	the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans	835:953	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	14	106	theme	colonic	2655:2661	arg1	cells					2674:2678	the microbial ecosystem and host colonic epithelial cells	2622:2678	cells	2674:2678	This promoted the microbial synthesis of acetate, propionate, and butyrate, which are beneficial to the microbial ecosystem and host colonic epithelial cells.
30674645	5	107	theme	AcAGX	993:997	arg1	potential					970:978	The prebiotic potential	956:978	The prebiotic potential of AcGGM and AcAGX	956:997	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	8	108	theme	increase	1390:1397	arg1	exception					1374:1382	the exception	1370:1382	the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05)	1370:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	16	109	theme	pivotal	2825:2831	arg1	requirements					2833:2844	pivotal requirements	2825:2844	pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health	2825:2982	These findings represent pivotal requirements for rationally designing intervention strategies based on the dietary supplementation of AcGGM and AcAGX to improve or restore gut health.
30674645	4	110	theme	Bifidobacterium	751:765	arg1	Lactobacillus					768:780	Bifidobacterium, Lactobacillus, and Bacteroides species	751:805	Lactobacillus	768:780	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	0	111	theme	Dietary	13:19	arg1	Fibers					21:26	Wood-Derived Dietary Fibers	0:26	Wood-Derived Dietary Fibers	0:26	Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota.
30674645	5	112	theme	prebiotic	960:968	arg1	potential					970:978	The prebiotic potential	956:978	The prebiotic potential of AcGGM and AcAGX	956:997	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	3	113	with	similarity	321:330	arg1	fibers					352:357	common dietary fibers	337:357	common dietary fibers	337:357	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	4	114	theme	prebiotic	543:551	arg1	potential					553:561	the prebiotic potential	539:561	the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture	539:697	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	3	115	theme	health	433:438	arg1	benefit					440:446	a health benefit	431:446	a health benefit	431:446	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	4	116	theme	Bacteroides	787:797	arg1	species					799:805	Bifidobacterium, Lactobacillus, and Bacteroides species	751:805	species	799:805	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	7	117	dep	increased	1262:1270	arg1	P < 0.05					1273:1280	P < 0.05	1273:1280	P < 0.05	1273:1280	Similarly to FOS, both substrates significantly increased (P < 0.05) the Bifidobacterium population.
30674645	3	118	theme	specific	468:475	arg1	modulation					477:486	specific modulation	468:486	specific modulation of the gut microbiota	468:508	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	0	119	theme	Human	47:51	arg1	Microbiota					57:66	Beneficial Human Gut Microbiota	36:66	Beneficial Human Gut Microbiota	36:66	Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota.
30674645	5	120	theme	batch	1039:1043	arg1	system					1066:1071	a pH-controlled batch culture fermentation system	1023:1071	a pH-controlled batch culture fermentation system inoculated with healthy adult human feces	1023:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	8	121	theme	Bacteroides-Prevotella	1431:1452	arg1	group					1454:1458	the Bacteroides-Prevotella group	1427:1458	the Bacteroides-Prevotella group	1427:1458	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	5	122	theme	fermentation	1053:1064	arg1	system					1066:1071	a pH-controlled batch culture fermentation system	1023:1071	a pH-controlled batch culture fermentation system inoculated with healthy adult human feces	1023:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	6	123	theme	commercial	1165:1174	arg1	mixture					1205:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	a commercial fructo-oligosaccharide (FOS) mixture	1163:1211	Results were compared with those obtained with a commercial fructo-oligosaccharide (FOS) mixture.
30674645	3	124	theme	gut	495:497	arg1	microbiota					499:508	the gut microbiota	491:508	the gut microbiota	491:508	Based on the structure similarity with common dietary fibers, GGM and AGX may be postulated to have prebiotic properties, conferring a health benefit on the host through specific modulation of the gut microbiota.
30674645	8	125	from	growth	1406:1411	arg1	exception					1374:1382	the exception	1370:1382	the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05)	1370:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	5	126	theme	pH-controlled	1025:1037	arg1	system					1066:1071	a pH-controlled batch culture fermentation system	1023:1071	a pH-controlled batch culture fermentation system inoculated with healthy adult human feces	1023:1113	The prebiotic potential of AcGGM and AcAGX was further assessed in a pH-controlled batch culture fermentation system inoculated with healthy adult human feces.
30674645	13	127	theme	Bacteroides-Prevotella	2451:2472	arg1	spp					2518:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	12	128	theme	gut	2320:2322	arg1	composition					2335:2345	human gut microbiota composition	2314:2345	human gut microbiota composition	2314:2345	This work provides insights into the ability of two novel wood-derived preparations, AcGGM and AcAGX, to influence human gut microbiota composition and activity.
30674645	10	129	theme	bacterial	1954:1962	arg1	ecosystem					1964:1972	the gut bacterial ecosystem	1946:1972	the gut bacterial ecosystem	1946:1972	Although further in vivo confirmation is necessary, these results demonstrate that both AcGGM and AcAGX from lignocellulosic feedstocks can be used to direct the promotion of beneficial bacteria, thus exhibiting a promising prebiotic ability to improve or restore gut health.IMPORTANCE The architecture of the gut bacterial ecosystem has a profound effect on the physiology and well-being of the host.
30674645	8	130	theme	prausnitzii	1478:1488	arg1	exception					1374:1382	the exception	1370:1382	the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05)	1370:1527	Other bacterial groups enumerated were unaffected with the exception of an increase in the growth of members of the Bacteroides-Prevotella group, Faecalibacterium prausnitzii, and clostridial cluster IX (P < 0.05).
30674645	13	131	theme	F.	2475:2476	arg1	spp					2518:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp	2434:2520	These compounds were selectively fermented by commensal bacteria such as Bifidobacterium, Bacteroides-Prevotella, F. prausnitzii, and clostridial cluster IX spp.
30674645	7	132	theme	Bifidobacterium	1287:1301	arg1	population					1303:1312	the Bifidobacterium population	1283:1312	the Bifidobacterium population	1283:1312	Similarly to FOS, both substrates significantly increased (P < 0.05) the Bifidobacterium population.
30674645	4	133	theme	acetylated	600:609	arg1	AcAGX					616:620	AcAGX	616:620	AcAGX	616:620	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30674645	4	133	theme	acetylated	600:609	arg1	AGX					611:613	highly acetylated AGX	593:613	highly acetylated AGX (AcAGX)	593:621	In this study, we evaluated the prebiotic potential of acetylated GGM (AcGGM) and highly acetylated AGX (AcAGX) obtained from Norwegian lignocellulosic feedstocks in vitro In pure culture, both substrates selectively promoted the growth of Bifidobacterium, Lactobacillus, and Bacteroides species in a manner consistent with the presence of genetic loci for the utilization of β-manno-oligosaccharides/β-mannans and xylo-oligosaccharides/xylans.
30955601	2	0	theme	pathogens	607:615	arg1	promotion					540:548	promotion	540:548	promotion of intestinal probiotics proliferation	540:587	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	2	0	theme	pathogens	607:615	arg1	inhibition					593:602	inhibition	593:602	inhibition of pathogens	593:615	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	3	1	contain	contained	711:719	arg1	concentration					644:656	the final concentration	634:656	the final concentration of total SCFAs	634:671	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	3	1	contain	contained	711:719	arg2	amounts					726:732	high amounts	721:732	high amounts of acetic, propionic and butyric acid	721:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	3	1	contain	contained	711:719	arg2	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	2	2	theme	diversity	456:464	arg1	level					436:440	the level	432:440	the level of microflora diversity	432:464	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	6	3	theme	human	1095:1099	arg1	tract					1118:1122	the human gastrointestinal tract	1091:1122	the human gastrointestinal tract	1091:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	3	4	theme	final	638:642	arg1	concentration					644:656	the final concentration	634:656	the final concentration of total SCFAs	634:671	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	1	5	theme	microbiota	251:260	arg1	composition					262:272	microbiota composition	251:272	microbiota composition	251:272	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	5	6	dep	9	943:943	arg1	to					940:941	to	940:941	to	940:941	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	5	7	theme	linkage	960:966	arg1	1 → 3					983:987	1 → 3	983:987	1 → 3	983:987	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	5	7	theme	linkage	960:966	arg1	patterns					968:975	the main linkage patterns	951:975	the main linkage patterns	951:975	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	1	8	theme	composition	262:272	arg1	variations					237:246	the variations	233:246	the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs)	233:329	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	2	9	theme	microbiome	498:507	arg1	structure					509:517	the microbiome structure	494:517	the microbiome structure	494:517	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	2	10	dep	promotion	540:548	arg1	growth					617:622	growth	617:622	growth	617:622	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	3	11	theme	acetic	737:742	arg1	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	6	12	theme	PHP	1080:1082	arg1	activity					1068:1075	the probiotic activity	1054:1075	the probiotic activity of PHP within the human gastrointestinal tract	1054:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	5	13	theme	polymerization	883:896	arg1	degree					873:878	The degree	869:878	The degree of polymerization of oligosaccharide products	869:924	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	1	14	theme	Porphyra	163:170	arg1	polysaccharides					184:198	Porphyra haitanensis polysaccharides	163:198	Porphyra haitanensis polysaccharides (PHP)	163:204	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	14	theme	Porphyra	163:170	arg1	PHP					201:203	PHP	201:203	PHP	201:203	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	3	15	theme	high	721:724	arg1	amounts					726:732	high amounts	721:732	high amounts of acetic, propionic and butyric acid	721:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	3	15	theme	high	721:724	arg1	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	2	16	theme	microflora	445:454	arg1	diversity					456:464	microflora diversity	445:464	microflora diversity	445:464	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	6	17	from	insight	1043:1049	arg1	activity					1068:1075	the probiotic activity	1054:1075	the probiotic activity of PHP within the human gastrointestinal tract	1054:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	0	18	theme	Microbial	0:8	arg1	catabolism					10:19	Microbial catabolism	0:19	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.	0:84	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	3	19	theme	propionic	745:753	arg1	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	1	20	theme	polysaccharides	184:198	arg1	catabolism					149:158	the catabolism	145:158	the catabolism of Porphyra haitanensis polysaccharides (PHP)	145:204	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	21	from	effects	352:358	arg1	microbiota					387:396	human intestinal microbiota	370:396	human intestinal microbiota	370:396	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	6	22	theme	probiotic	1058:1066	arg1	activity					1068:1075	the probiotic activity	1054:1075	the probiotic activity of PHP within the human gastrointestinal tract	1054:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	0	23	theme	Porphyra	24:31	arg1	polysaccharides					45:59	Porphyra haitanensis polysaccharides	24:59	Porphyra haitanensis polysaccharides	24:59	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	1	24	theme	concentration	282:294	arg1	variations					237:246	the variations	233:246	the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs)	233:329	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	5	25	theme	main	955:958	arg1	1 → 3					983:987	1 → 3	983:987	1 → 3	983:987	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	5	25	theme	main	955:958	arg1	patterns					968:975	the main linkage patterns	951:975	the main linkage patterns	951:975	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	2	26	theme	24 h	405:408	arg1	incubation					420:429	24 h anaerobic incubation	405:429	24 h anaerobic incubation	405:429	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	1	27	theme	in	108:109	arg1	model					130:134	an in vitro fermentation model	105:134	an in vitro fermentation model	105:134	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	6	28	theme	gastrointestinal	1101:1116	arg1	tract					1118:1122	the human gastrointestinal tract	1091:1122	the human gastrointestinal tract	1091:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	0	29	theme	polysaccharides	45:59	arg1	catabolism					10:19	Microbial catabolism	0:19	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.	0:84	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	1	30	theme	short-chain	299:309	arg1	acids					317:321	short-chain fatty acids	299:321	short-chain fatty acids (SCFAs)	299:329	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	30	theme	short-chain	299:309	arg1	SCFAs					324:328	SCFAs	324:328	SCFAs	324:328	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	31	theme	PHP	363:365	arg1	effects					352:358	the effects	348:358	the effects of PHP on human intestinal microbiota	348:396	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	0	32	theme	haitanensis	33:43	arg1	polysaccharides					45:59	Porphyra haitanensis polysaccharides	24:59	Porphyra haitanensis polysaccharides	24:59	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	1	33	theme	fatty	311:315	arg1	acids					317:321	short-chain fatty acids	299:321	short-chain fatty acids (SCFAs)	299:329	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	33	theme	fatty	311:315	arg1	SCFAs					324:328	SCFAs	324:328	SCFAs	324:328	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	2	34	theme	anaerobic	410:418	arg1	incubation					420:429	24 h anaerobic incubation	405:429	24 h anaerobic incubation	405:429	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	6	35	theme	new	1039:1041	arg1	insight					1043:1049	new insight	1039:1049	new insight on the probiotic activity of PHP within the human gastrointestinal tract	1039:1122	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	0	36	theme	human	64:68	arg1	microbiota					74:83	human gut microbiota	64:83	human gut microbiota	64:83	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	1	37	theme	acids	317:321	arg1	composition					262:272	microbiota composition	251:272	microbiota composition	251:272	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	37	theme	acids	317:321	arg1	concentration					282:294	the concentration	278:294	the concentration of short-chain fatty acids (SCFAs)	278:329	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	2	38	theme	proliferation	575:587	arg1	promotion					540:548	promotion	540:548	promotion of intestinal probiotics proliferation	540:587	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	2	38	theme	proliferation	575:587	arg1	inhibition					593:602	inhibition	593:602	inhibition of pathogens	593:615	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	1	39	theme	human	370:374	arg1	microbiota					387:396	human intestinal microbiota	370:396	human intestinal microbiota	370:396	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	40	dep	Porphyra	163:170	arg1	haitanensis					172:182	haitanensis	172:182	haitanensis	172:182	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	3	41	theme	butyric	759:765	arg1	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	3	42	theme	acid	767:770	arg1	amounts					726:732	high amounts	721:732	high amounts of acetic, propionic and butyric acid	721:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	3	42	theme	acid	767:770	arg1	acid					767:770	acetic, propionic and butyric acid	737:770	acetic, propionic and butyric acid	737:770	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	2	43	theme	probiotics	564:573	arg1	proliferation					575:587	intestinal probiotics proliferation	553:587	intestinal probiotics proliferation	553:587	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	1	44	theme	intestinal	376:385	arg1	microbiota					387:396	human intestinal microbiota	370:396	human intestinal microbiota	370:396	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	1	45	theme	fermentation	117:128	arg1	model					130:134	an in vitro fermentation model	105:134	an in vitro fermentation model	105:134	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
30955601	2	46	theme	intestinal	553:562	arg1	proliferation					575:587	intestinal probiotics proliferation	553:587	intestinal probiotics proliferation	553:587	After 24 h anaerobic incubation, the level of microflora diversity increased significantly, as the microbiome structure was reshaped through promotion of intestinal probiotics proliferation and inhibition of pathogens growth.
30955601	4	47	theme	molecular	790:798	arg1	weight					800:805	the molecular weight	786:805	the molecular weight of PHP	786:812	Furthermore, the molecular weight of PHP decreased from 2.623 × 105 g/mol to 2.308 × 104 g/mol.
30955601	4	48	theme	PHP	810:812	arg1	weight					800:805	the molecular weight	786:805	the molecular weight of PHP	786:812	Furthermore, the molecular weight of PHP decreased from 2.623 × 105 g/mol to 2.308 × 104 g/mol.
30955601	3	49	theme	total	661:665	arg1	SCFAs					667:671	total SCFAs	661:671	total SCFAs	661:671	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	5	50	theme	linked	999:1004	arg1	Galp					1006:1009	1 → 4 linked Galp	993:1009	1 → 4 linked Galp	993:1009	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	5	51	theme	products	917:924	arg1	polymerization					883:896	polymerization	883:896	polymerization of oligosaccharide products	883:924	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	3	52	theme	SCFAs	667:671	arg1	concentration					644:656	the final concentration	634:656	the final concentration of total SCFAs	634:671	Besides, the final concentration of total SCFAs increased to 32.32 ± 1.81 mmol/L, and contained high amounts of acetic, propionic and butyric acid.
30955601	5	53	theme	oligosaccharide	901:915	arg1	products					917:924	oligosaccharide products	901:924	oligosaccharide products	901:924	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	6	54	theme	current	1016:1022	arg1	study					1024:1028	The current study	1012:1028	The current study	1012:1028	The current study provides new insight on the probiotic activity of PHP within the human gastrointestinal tract.
30955601	0	55	theme	gut	70:72	arg1	microbiota					74:83	human gut microbiota	64:83	human gut microbiota	64:83	Microbial catabolism of Porphyra haitanensis polysaccharides by human gut microbiota.
30955601	5	56	link	linked	999:1004	arg1	Galp					1006:1009	1 → 4 linked Galp	993:1009	1 → 4 linked Galp	993:1009	The degree of polymerization of oligosaccharide products ranged from 2 to 9, with the main linkage patterns being 1 → 3 and 1 → 4 linked Galp.
30955601	1	57	dep	in	108:109	arg1	vitro					111:115	vitro	111:115	vitro	111:115	This study applied an in vitro fermentation model, whereby the catabolism of Porphyra haitanensis polysaccharides (PHP) was monitored coupled with the variations of microbiota composition and the concentration of short-chain fatty acids (SCFAs), so as to assess the effects of PHP on human intestinal microbiota.
29642422	0	0	from	Characterization	9:24	arg1	Cells					89:93	Neuronal Cells	80:93	Neuronal Cells	80:93	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	6	1	theme	neuronal	1128:1135	arg1	model					1137:1141	a neuronal model	1126:1141	a neuronal model	1126:1141	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	2	2	from	encapsulation	288:300	arg1	matrix					329:334	maltodextrin/arabic gum matrix	305:334	maltodextrin/arabic gum matrix	305:334	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	6	3	theme	reactive	1077:1084	arg1	ROS					1102:1104	ROS	1102:1104	ROS	1102:1104	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	6	3	theme	reactive	1077:1084	arg1	species					1093:1099	reactive oxygen species	1077:1099	reactive oxygen species (ROS) analysis utilizing a neuronal model	1077:1141	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	6	4	contain	has	1022:1024	arg1	powder					1015:1020	the encapsulated red wine powder	989:1020	the encapsulated red wine powder	989:1020	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	6	4	contain	has	1022:1024	arg2	properties					1039:1048	active redox properties	1026:1048	active redox properties	1026:1048	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	4	5	theme	gallic	811:816	arg1	acid					818:821	gallic acid	811:821	gallic acid	811:821	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
29642422	3	6	theme	phenolic	567:574	arg1	acids					576:580	phenolic acids	567:580	phenolic acids	567:580	More than thirty polyphenols, including anthocyanins, flavanols, flavonols, phenolic acids and stilbenoids, were identified.
29642422	0	7	theme	Neuronal	80:87	arg1	Cells					89:93	Neuronal Cells	80:93	Neuronal Cells	80:93	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	5	8	theme	wine	910:913	arg1	activity					857:864	The biological activity	842:864	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells	842:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	5	9	theme	encapsulated	893:904	arg1	wine					910:913	this de-alcoholized and encapsulated red wine	869:913	this de-alcoholized and encapsulated red wine	869:913	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	4	10	theme	main	628:631	arg1	polyphenols					644:654	the main quantified polyphenols	624:654	the main quantified polyphenols	624:654	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
29642422	2	11	theme	wine	246:249	arg1	powder					251:256	a red wine powder	240:256	a red wine powder	240:256	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	1	12	theme	human	154:158	arg1	protection					167:176	human health protection	154:176	human health protection	154:176	Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability.
29642422	6	13	theme	redox	1033:1037	arg1	properties					1039:1048	active redox properties	1026:1048	active redox properties	1026:1048	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	5	14	theme	biological	846:855	arg1	activity					857:864	The biological activity	842:864	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells	842:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	0	15	theme	Chemical	0:7	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of an Encapsulated Red Wine Powder	0:59	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	1	16	theme	health	160:165	arg1	protection					167:176	human health protection	154:176	human health protection	154:176	Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability.
29642422	2	17	theme	mass	457:460	arg1	HPLC-MS-QTOF					476:487	HPLC-MS-QTOF	476:487	HPLC-MS-QTOF	476:487	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	2	17	theme	mass	457:460	arg1	spectrometry					462:473	quadrupole time-of-flight mass spectrometry	431:473	quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF)	431:488	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	5	18	from	activity	857:864	arg1	cells					946:950	human neuroblastoma SH-SY5Y cells	918:950	human neuroblastoma SH-SY5Y cells	918:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	5	19	theme	human	918:922	arg1	cells					946:950	human neuroblastoma SH-SY5Y cells	918:950	human neuroblastoma SH-SY5Y cells	918:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	2	20	theme	time-of-flight	442:455	arg1	HPLC-MS-QTOF					476:487	HPLC-MS-QTOF	476:487	HPLC-MS-QTOF	476:487	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	2	20	theme	time-of-flight	442:455	arg1	spectrometry					462:473	quadrupole time-of-flight mass spectrometry	431:473	quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF)	431:488	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	6	21	theme	active	1026:1031	arg1	properties					1039:1048	active redox properties	1026:1048	active redox properties	1026:1048	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	2	22	theme	freeze-drying	274:286	arg1	encapsulation					288:300	freeze-drying encapsulation	274:300	freeze-drying encapsulation in maltodextrin/arabic gum matrix	274:334	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	5	23	theme	neuroblastoma	924:936	arg1	cells					946:950	human neuroblastoma SH-SY5Y cells	918:950	human neuroblastoma SH-SY5Y cells	918:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	2	24	theme	quadrupole	431:440	arg1	HPLC-MS-QTOF					476:487	HPLC-MS-QTOF	476:487	HPLC-MS-QTOF	476:487	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	2	24	theme	quadrupole	431:440	arg1	spectrometry					462:473	quadrupole time-of-flight mass spectrometry	431:473	quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF)	431:488	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	6	25	theme	species	1093:1099	arg1	analysis					1107:1114	reactive oxygen species (ROS) analysis	1077:1114	reactive oxygen species (ROS) analysis utilizing a neuronal model	1077:1141	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	4	26	theme	quantified	633:642	arg1	polyphenols					644:654	the main quantified polyphenols	624:654	the main quantified polyphenols	624:654	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
29642422	1	27	theme	Red	96:98	arg1	polyphenols					105:115	Red wine polyphenols	96:115	Red wine polyphenols	96:115	Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability.
29642422	2	28	theme	liquid	401:406	arg1	chromatography					408:421	high-performance liquid chromatography	384:421	high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF)	384:488	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	0	29	theme	Red	45:47	arg1	Powder					54:59	an Encapsulated Red Wine Powder	29:59	an Encapsulated Red Wine Powder	29:59	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	6	30	theme	wine	1010:1013	arg1	powder					1015:1020	the encapsulated red wine powder	989:1020	the encapsulated red wine powder	989:1020	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	0	31	from	Effects	69:75	arg1	Cells					89:93	Neuronal Cells	80:93	Neuronal Cells	80:93	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	2	32	theme	high-performance	384:399	arg1	chromatography					408:421	high-performance liquid chromatography	384:421	high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF)	384:488	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	0	33	theme	Encapsulated	32:43	arg1	Powder					54:59	an Encapsulated Red Wine Powder	29:59	an Encapsulated Red Wine Powder	29:59	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	6	34	theme	red	1006:1008	arg1	powder					1015:1020	the encapsulated red wine powder	989:1020	the encapsulated red wine powder	989:1020	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	5	35	theme	red	906:908	arg1	wine					910:913	this de-alcoholized and encapsulated red wine	869:913	this de-alcoholized and encapsulated red wine	869:913	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	0	36	theme	Powder	54:59	arg1	Effects					69:75	Its Effects	65:75	Its Effects on Neuronal Cells	65:93	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	0	36	theme	Powder	54:59	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of an Encapsulated Red Wine Powder	0:59	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	6	37	theme	encapsulated	993:1004	arg1	powder					1015:1020	the encapsulated red wine powder	989:1020	the encapsulated red wine powder	989:1020	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	2	38	dep	chromatography	408:421	arg1	means					375:379	means	375:379	means	375:379	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	1	39	theme	wine	100:103	arg1	polyphenols					105:115	Red wine polyphenols	96:115	Red wine polyphenols	96:115	Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability.
29642422	0	40	theme	Wine	49:52	arg1	Powder					54:59	an Encapsulated Red Wine Powder	29:59	an Encapsulated Red Wine Powder	29:59	Chemical Characterization of an Encapsulated Red Wine Powder and Its Effects on Neuronal Cells.
29642422	2	41	theme	gum	325:327	arg1	matrix					329:334	maltodextrin/arabic gum matrix	305:334	maltodextrin/arabic gum matrix	305:334	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	2	42	theme	maltodextrin/arabic	305:323	arg1	matrix					329:334	maltodextrin/arabic gum matrix	305:334	maltodextrin/arabic gum matrix	305:334	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	1	43	theme	high	205:208	arg1	instability					210:220	high instability	205:220	high instability	205:220	Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability.
29642422	2	44	theme	red	242:244	arg1	wine					246:249	a red wine	240:249	a red wine powder	240:256	For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF).
29642422	4	45	theme	syringic	827:834	arg1	acid					836:839	syringic acid	827:839	syringic acid	827:839	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
29642422	6	46	theme	oxygen	1086:1091	arg1	ROS					1102:1104	ROS	1102:1104	ROS	1102:1104	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	6	46	theme	oxygen	1086:1091	arg1	species					1093:1099	reactive oxygen species	1077:1099	reactive oxygen species (ROS) analysis utilizing a neuronal model	1077:1141	The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model.
29642422	5	47	theme	de-alcoholized	874:887	arg1	wine					910:913	this de-alcoholized and encapsulated red wine	869:913	this de-alcoholized and encapsulated red wine	869:913	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	5	48	theme	SH-SY5Y	938:944	arg1	cells					946:950	human neuroblastoma SH-SY5Y cells	918:950	human neuroblastoma SH-SY5Y cells	918:950	The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied.
29642422	4	49	theme	3-O-	695:698	arg1	6″-acetyl-glucose					700:716	6″-acetyl-glucose	700:716	6″-acetyl-glucose	700:716	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
29642422	4	49	theme	3-O-	695:698	arg1	malvidin					686:693	malvidin 3-O-	686:698	malvidin 3-O-(6″-acetyl-glucose)	686:717	Some of the main quantified polyphenols were: malvidin-3-O-glucoside, malvidin 3-O-(6″-acetyl-glucose), petunidin-3-O-glucoside, quercetin-3-O-glucuronide, syringenin-3-O-glucoside, epicatechin, gallic acid and syringic acid.
31554089	4	0	theme	different	670:678	arg1	processes					699:707	two different in vitro digestion processes	666:707	two different in vitro digestion processes	666:707	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	1	1	from	compounds	166:174	arg1	rich					149:152	rich	149:152	rich	149:152	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	2	theme	rich	149:152	arg1	fruit					143:147	a native Brazilian fruit	124:147	a native Brazilian fruit rich in phenolic compounds as anthocyanins	124:190	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	2	theme	rich	149:152	arg1	Jabuticaba					110:119	Jabuticaba	110:119	Jabuticaba	110:119	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	8	3	theme	good	1433:1436	arg1	carriers					1438:1445	good carriers	1433:1445	good carriers for anthocyanins	1433:1462	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	8	3	theme	good	1433:1436	arg1	gels					1415:1418	gellan gels	1408:1418	gellan gels	1408:1418	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	1	4	from	rich	149:152	arg1	compounds					166:174	phenolic compounds	157:174	phenolic compounds as anthocyanins	157:190	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	9	5	theme	shear	1865:1869	arg1	forces					1871:1876	shear forces	1865:1876	compression (a more similar-to-in vivo system) rather than shear forces	1806:1876	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	1	6	theme	high	241:244	arg1	sensibility					246:256	a high sensibility	239:256	a high sensibility to physicochemical digestion conditions	239:296	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	0	7	theme	matrix-bioactive	80:95	arg1	interaction					97:107	matrix-bioactive interaction	80:107	matrix-bioactive interaction	80:107	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	3	8	contain	containing	491:500	arg1	gels					486:489	gellan gels	479:489	gellan gels containing jabuticaba extract	479:519	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	3	8	contain	containing	491:500	arg2	extract					513:519	jabuticaba extract	502:519	jabuticaba extract	502:519	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	6	9	from	effect	1017:1022	arg1	properties					1054:1063	gels mechanical-structural properties	1027:1063	gels mechanical-structural properties	1027:1063	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	4	10	theme	mechanical	732:741	arg1	forces					743:748	mechanical forces	732:748	mechanical forces applied to simulate stomach and intestine movement conditions	732:810	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	4	11	dep	in	680:681	arg1	vitro					683:687	vitro	683:687	vitro	683:687	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	6	12	theme	strain	1134:1139	arg1	decrease					1122:1129	a decrease	1120:1129	a decrease of strain at rupture (deformability)	1120:1166	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	12	theme	strain	1134:1139	arg1	increase					1075:1082	an increase	1072:1082	an increase of stress at rupture (hardness)	1072:1114	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	5	13	theme	in	946:947	arg1	digestibility					955:967	in vitro digestibility	946:967	in vitro digestibility	946:967	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	3	14	theme	gellan	479:484	arg1	gels					486:489	gellan gels	479:489	gellan gels containing jabuticaba extract	479:519	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	6	15	from	rupture	1144:1150	arg1	decrease					1122:1129	a decrease	1120:1129	a decrease of strain at rupture (deformability)	1120:1166	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	15	from	rupture	1144:1150	arg1	increase					1075:1082	an increase	1072:1082	an increase of stress at rupture (hardness)	1072:1114	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	4	16	theme	digestion	689:697	arg1	processes					699:707	two different in vitro digestion processes	666:707	two different in vitro digestion processes	666:707	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	8	17	theme	matrix	1506:1511	arg1	composition					1513:1523	the matrix composition	1502:1523	the matrix composition	1502:1523	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	6	18	theme	stress	1087:1092	arg1	decrease					1122:1129	a decrease	1120:1129	a decrease of strain at rupture (deformability)	1120:1166	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	18	theme	stress	1087:1092	arg1	increase					1075:1082	an increase	1072:1082	an increase of stress at rupture (hardness)	1072:1114	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	9	19	theme	forces	1871:1876	arg1	use					1799:1801	the use	1795:1801	the use of compression (a more similar-to-in vivo system) rather than shear forces	1795:1876	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	2	20	theme	bioactive	376:384	arg1	compounds					386:394	bioactive compounds	376:394	bioactive compounds	376:394	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	0	21	theme	interaction	97:107	arg1	Effect					70:75	Effect	70:75	Effect of matrix-bioactive interaction	70:107	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	9	22	theme	compression	1806:1816	arg1	use					1799:1801	the use	1795:1801	the use of compression (a more similar-to-in vivo system) rather than shear forces	1795:1876	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	7	23	theme	simulated	1290:1298	arg1	process					1327:1333	simulated gastrointestinal digestion process	1290:1333	simulated gastrointestinal digestion process	1290:1333	Although all gellan gels have improved anthocyanins retention during simulated gastrointestinal digestion process, gels without calcium were more efficient.
31554089	4	24	theme	forces	743:748	arg1	type					724:727	the type	720:727	the type of mechanical forces applied to simulate stomach and intestine movement conditions	720:810	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	5	25	theme	differential	841:852	arg1	method					854:859	pH differential method	838:859	pH differential method	838:859	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	7	26	theme	anthocyanins	1260:1271	arg1	retention					1273:1281	anthocyanins retention	1260:1281	anthocyanins retention during simulated gastrointestinal digestion process	1260:1333	Although all gellan gels have improved anthocyanins retention during simulated gastrointestinal digestion process, gels without calcium were more efficient.
31554089	6	27	located	observed	1173:1180	arg2	increase					1075:1082	an increase	1072:1082	an increase of stress at rupture (hardness)	1072:1114	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	27	located	observed	1173:1180	arg1	gels					1190:1193	gels	1190:1193	gels	1190:1193	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	27	located	observed	1173:1180	arg2	decrease					1122:1129	a decrease	1120:1129	a decrease of strain at rupture (deformability)	1120:1166	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	3	28	theme	ionic	555:559	arg1	values					570:575	two different ionic strength values	541:575	two different ionic strength values (adding calcium ions)	541:597	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	9	29	theme	forces	1722:1727	arg1	type					1703:1706	the type	1699:1706	the type of mechanical forces applied during in vitro digestion	1699:1761	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	9	29	theme	forces	1722:1727	arg1	variable					1779:1786	an important variable	1766:1786	an important variable	1766:1786	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	7	30	theme	gastrointestinal	1300:1315	arg1	process					1327:1333	simulated gastrointestinal digestion process	1290:1333	simulated gastrointestinal digestion process	1290:1333	Although all gellan gels have improved anthocyanins retention during simulated gastrointestinal digestion process, gels without calcium were more efficient.
31554089	0	31	theme	In	0:1	arg1	digestibility					9:21	In vitro digestibility	0:21	In vitro digestibility of gellan gels	0:36	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	1	32	theme	physicochemical	261:275	arg1	conditions					287:296	physicochemical digestion conditions	261:296	physicochemical digestion conditions	261:296	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	5	33	theme	pH	838:839	arg1	method					854:859	pH differential method	838:859	pH differential method	838:859	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	3	34	theme	strength	561:568	arg1	values					570:575	two different ionic strength values	541:575	two different ionic strength values (adding calcium ions)	541:597	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	9	35	theme	anthocyanins	1903:1914	arg1	release					1892:1898	the release	1888:1898	the release of anthocyanins	1888:1914	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	7	36	theme	digestion	1317:1325	arg1	process					1327:1333	simulated gastrointestinal digestion process	1290:1333	simulated gastrointestinal digestion process	1290:1333	Although all gellan gels have improved anthocyanins retention during simulated gastrointestinal digestion process, gels without calcium were more efficient.
31554089	9	37	theme	important	1769:1777	arg1	type					1703:1706	the type	1699:1706	the type of mechanical forces applied during in vitro digestion	1699:1761	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	9	37	theme	important	1769:1777	arg1	variable					1779:1786	an important variable	1766:1786	an important variable	1766:1786	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	1	38	theme	digestion	277:285	arg1	conditions					287:296	physicochemical digestion conditions	261:296	physicochemical digestion conditions	261:296	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	2	39	used	used	336:339	arg2	biopolymer					311:320	a biopolymer	309:320	a biopolymer that could be used as a material to protect and carry bioactive compounds	309:394	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	2	39	used	used	336:339	arg2	material					346:353	a material	344:353	a material	344:353	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	2	39	used	used	336:339	arg2	Gellan					299:304	Gellan	299:304	Gellan	299:304	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	6	40	theme	calcium	1203:1209	arg1	addition					1211:1218	calcium addition	1203:1218	calcium addition	1203:1218	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	5	41	theme	gels	919:922	arg1	Anthocyanins					813:824	Anthocyanins	813:824	Anthocyanins	813:824	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	5	41	theme	gels	919:922	arg1	microscopy					905:914	confocal and light microscopy	886:914	microscopy	905:914	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	3	42	theme	varied	618:623	arg1	structures					625:634	varied structures	618:634	varied structures	618:634	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	3	43	theme	different	545:553	arg1	values					570:575	two different ionic strength values	541:575	two different ionic strength values (adding calcium ions)	541:597	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	6	44	theme	mechanical-structural	1032:1052	arg1	properties					1054:1063	gels mechanical-structural properties	1027:1063	gels mechanical-structural properties	1027:1063	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	0	45	theme	gels	33:36	arg1	digestibility					9:21	In vitro digestibility	0:21	In vitro digestibility of gellan gels	0:36	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	9	46	dep	in	1744:1745	arg1	vitro					1747:1751	vitro	1747:1751	vitro	1747:1751	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	6	47	theme	gels	1027:1030	arg1	properties					1054:1063	gels mechanical-structural properties	1027:1063	gels mechanical-structural properties	1027:1063	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	4	48	theme	movement	792:799	arg1	conditions					801:810	stomach and intestine movement conditions	770:810	stomach and intestine movement conditions	770:810	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	1	49	theme	several	201:207	arg1	benefits					209:216	several benefits	201:216	several benefits for human health	201:233	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	50	theme	phenolic	157:164	arg1	compounds					166:174	phenolic compounds	157:174	phenolic compounds as anthocyanins	157:190	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	0	51	theme	gellan	26:31	arg1	gels					33:36	gellan gels	26:36	gellan gels	26:36	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	2	52	contain	carry	370:374	arg1	material					346:353	a material	344:353	a material	344:353	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	2	52	contain	carry	370:374	arg2	compounds					386:394	bioactive compounds	376:394	bioactive compounds	376:394	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	2	52	contain	carry	370:374	arg1	Gellan					299:304	Gellan	299:304	Gellan	299:304	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	2	52	contain	carry	370:374	arg1	biopolymer					311:320	a biopolymer	309:320	a biopolymer that could be used as a material to protect and carry bioactive compounds	309:394	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	3	53	theme	calcium	585:591	arg1	ions					593:596	calcium ions	585:596	calcium ions	585:596	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	5	54	dep	in	946:947	arg1	vitro					949:953	vitro	949:953	vitro	949:953	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	2	55	theme	pH	447:448	arg1	conditions					450:459	gastric pH conditions	439:459	gastric pH conditions	439:459	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	9	56	theme	mechanical	1711:1720	arg1	forces					1722:1727	mechanical forces	1711:1727	mechanical forces applied during in vitro digestion	1711:1761	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	8	57	theme	gellan	1408:1413	arg1	carriers					1438:1445	good carriers	1433:1445	good carriers for anthocyanins	1433:1462	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	8	57	theme	gellan	1408:1413	arg1	gels					1415:1418	gellan gels	1408:1418	gellan gels	1408:1418	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	5	58	theme	confocal	886:893	arg1	Anthocyanins					813:824	Anthocyanins	813:824	Anthocyanins	813:824	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	5	58	theme	confocal	886:893	arg1	microscopy					905:914	confocal and light microscopy	886:914	microscopy	905:914	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	2	59	theme	gastric	439:445	arg1	conditions					450:459	gastric pH conditions	439:459	gastric pH conditions	439:459	Gellan is a biopolymer that could be used as a material to protect and carry bioactive compounds, since this polysaccharide is resistant to gastric pH conditions.
31554089	7	60	theme	gellan	1234:1239	arg1	gels					1241:1244	all gellan gels	1230:1244	all gellan gels	1230:1244	Although all gellan gels have improved anthocyanins retention during simulated gastrointestinal digestion process, gels without calcium were more efficient.
31554089	9	61	theme	similar-to-in	1826:1838	arg1	system					1845:1850	a more similar-to-in vivo system	1819:1850	a more similar-to-in vivo system	1819:1850	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	9	61	theme	similar-to-in	1826:1838	arg1	compression					1806:1816	compression	1806:1816	compression (a more similar-to-in vivo system) rather than shear forces	1806:1876	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	4	62	theme	stomach	770:776	arg1	conditions					801:810	stomach and intestine movement conditions	770:810	stomach and intestine movement conditions	770:810	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	8	63	theme	specific	1545:1552	arg1	studies					1554:1560	specific studies	1545:1560	specific studies	1545:1560	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	4	64	theme	intestine	782:790	arg1	conditions					801:810	stomach and intestine movement conditions	770:810	stomach and intestine movement conditions	770:810	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	5	65	theme	light	899:903	arg1	Anthocyanins					813:824	Anthocyanins	813:824	Anthocyanins	813:824	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	5	65	theme	light	899:903	arg1	microscopy					905:914	confocal and light microscopy	886:914	microscopy	905:914	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	8	66	theme	bioactive	1642:1650	arg1	addition					1652:1659	bioactive addition	1642:1659	bioactive addition	1642:1659	Our results demonstrated that gellan gels could act as good carriers for anthocyanins, but their efficiency is dependent on the matrix composition, demonstrating that specific studies should be accomplished to determine which changes may occur in the matrix after bioactive addition.
31554089	4	67	dep	different	670:678	arg1	in					680:681	in	680:681	in	680:681	These gels were subjected to two different in vitro digestion processes, modifying the type of mechanical forces applied to simulate stomach and intestine movement conditions.
31554089	1	68	theme	native	126:131	arg1	fruit					143:147	a native Brazilian fruit	124:147	a native Brazilian fruit rich in phenolic compounds as anthocyanins	124:190	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	68	theme	native	126:131	arg1	Jabuticaba					110:119	Jabuticaba	110:119	Jabuticaba	110:119	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	69	theme	human	222:226	arg1	health					228:233	human health	222:233	human health	222:233	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	9	70	theme	in	1744:1745	arg1	digestion					1753:1761	in vitro digestion	1744:1761	in vitro digestion	1744:1761	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	5	71	theme	mechanical	863:872	arg1	properties					874:883	mechanical properties	863:883	mechanical properties	863:883	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	5	71	theme	mechanical	863:872	arg1	Anthocyanins					813:824	Anthocyanins	813:824	Anthocyanins	813:824	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	5	72	dep	Anthocyanins	813:824	arg1	release					826:832	release	826:832	release (by pH differential method)	826:860	Anthocyanins release (by pH differential method), mechanical properties, confocal and light microscopy of gels were evaluated during in vitro digestibility.
31554089	3	73	dep	values	570:575	arg1	adding					578:583	adding	578:583	adding calcium ions	578:596	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	1	74	theme	Brazilian	133:141	arg1	fruit					143:147	a native Brazilian fruit	124:147	a native Brazilian fruit rich in phenolic compounds as anthocyanins	124:190	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	1	74	theme	Brazilian	133:141	arg1	Jabuticaba					110:119	Jabuticaba	110:119	Jabuticaba	110:119	Jabuticaba is a native Brazilian fruit rich in phenolic compounds as anthocyanins, showing several benefits for human health but a high sensibility to physicochemical digestion conditions.
31554089	9	75	theme	vivo	1840:1843	arg1	system					1845:1850	a more similar-to-in vivo system	1819:1850	a more similar-to-in vivo system	1819:1850	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	9	75	theme	vivo	1840:1843	arg1	compression					1806:1816	compression	1806:1816	compression (a more similar-to-in vivo system) rather than shear forces	1806:1876	Furthermore, our results showed that the type of mechanical forces applied during in vitro digestion is an important variable, since the use of compression (a more similar-to-in vivo system) rather than shear forces increased the release of anthocyanins.
31554089	0	76	theme	jabuticaba	50:59	arg1	extract					61:67	jabuticaba extract	50:67	jabuticaba extract	50:67	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	0	77	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro digestibility of gellan gels loaded with jabuticaba extract: Effect of matrix-bioactive interaction.
31554089	6	78	from	rupture	1097:1103	arg1	decrease					1122:1129	a decrease	1120:1129	a decrease of strain at rupture (deformability)	1120:1166	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	6	78	from	rupture	1097:1103	arg1	increase					1075:1082	an increase	1072:1082	an increase of stress at rupture (hardness)	1072:1114	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31554089	3	79	theme	jabuticaba	502:511	arg1	extract					513:519	jabuticaba extract	502:519	jabuticaba extract	502:519	In this context, gellan gels containing jabuticaba extract were produced using two different ionic strength values (adding calcium ions), which resulted in varied structures.
31554089	6	80	theme	jabuticaba	990:999	arg1	extract					1001:1007	jabuticaba extract	990:1007	jabuticaba extract	990:1007	Results showed that jabuticaba extract exerted effect on gels mechanical-structural properties, since an increase of stress at rupture (hardness) and a decrease of strain at rupture (deformability) were observed only in gels without calcium addition.
31284003	0	0	theme	cationic	78:85	arg1	dyes					87:90	cationic dyes	78:90	cationic dyes in aqueous solutions	78:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	2	1	from	wastewater	382:391	arg1	dyes					372:375	dyes	372:375	dyes from wastewater	372:391	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	2	1	from	wastewater	382:391	arg1	removal					361:367	the removal	357:367	the removal of dyes from wastewater	357:391	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	2	2	theme	water	326:330	arg1	resources					332:340	the water resources	322:340	the water resources	322:340	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	5	3	theme	isotherm	944:951	arg1	adsorption					953:962	the Langmuir isotherm adsorption	931:962	the Langmuir isotherm adsorption model	931:968	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	1	4	theme	industrialization	146:162	arg1	development					131:141	the rapid development	121:141	the rapid development of industrialization	121:162	Due to the rapid development of industrialization, the water resources on which we depend are facing unprecedented challenges.
31284003	9	5	theme	/graphite	1426:1434	arg1	nanocomposite					1442:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	3	6	from	wastewaters	581:591	arg1	paper					601:605	this paper	596:605	this paper	596:605	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	3	7	theme	/graphite	458:466	arg1	nanocomposite					474:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	5	8	theme	adsorption	953:962	arg1	model					964:968	the Langmuir isotherm adsorption model	931:968	the Langmuir isotherm adsorption model	931:968	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	4	9	dep	spectroscopy	661:672	arg1	i.e.					628:631	i.e.	628:631	i.e.	628:631	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	9	10	theme	oxide	1436:1440	arg1	nanocomposite					1442:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	0	11	theme	dyes	87:90	arg1	remediation					63:73	remediation	63:73	remediation of cationic dyes in aqueous solutions	63:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	4	12	theme	elemental	722:730	arg1	analysis					732:739	elemental analysis	722:739	elemental analysis	722:739	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	4	13	theme	infrared	652:659	arg1	spectroscopy					661:672	Fourier-transform infrared spectroscopy	634:672	Fourier-transform infrared spectroscopy	634:672	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	9	14	theme	cationic	1486:1493	arg1	removal					1499:1505	cationic dye removal	1486:1505	cationic dye removal	1486:1505	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	4	15	theme	X-ray	675:679	arg1	diffraction					681:691	X-ray diffraction	675:691	X-ray diffraction	675:691	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	9	16	theme	acrylic	1413:1419	arg1	xylan/poly					1401:1410	porous xylan/poly	1394:1410	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	9	16	theme	acrylic	1413:1419	arg1	acid					1421:1424	acrylic acid	1413:1424	acrylic acid	1413:1424	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	2	17	from	pollution	309:317	arg1	nature					345:350	nature	345:350	nature	345:350	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	2	18	theme	indispensable	253:265	arg1	substance					267:275	an indispensable substance	250:275	an indispensable substance in our lives	250:288	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	5	19	theme	adsorption	906:915	arg1	behavior					917:924	the composite's adsorption behavior	890:924	the composite's adsorption behavior	890:924	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	4	20	theme	Various	608:614	arg1	techniques					616:625	Various techniques	608:625	Various techniques	608:625	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	0	21	theme	aqueous	95:101	arg1	solutions					103:111	aqueous solutions	95:111	aqueous solutions	95:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	9	22	contain	has	1456:1458	arg2	applications					1470:1481	potential applications	1460:1481	potential applications	1460:1481	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	9	22	contain	has	1456:1458	arg1	nanocomposite					1442:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	9	23	theme	xylan/poly	1401:1410	arg1	nanocomposite					1442:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	6	24	theme	excellent	1018:1026	arg1	properties					1039:1048	excellent adsorption properties	1018:1048	excellent adsorption properties	1018:1048	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	6	25	theme	violet	1093:1098	arg1	dye					1100:1102	ethyl violet dye	1087:1102	ethyl violet dye	1087:1102	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	8	26	theme	cycling	1261:1267	arg1	tests					1269:1273	Desorption cycling tests	1250:1273	Desorption cycling tests	1250:1273	Desorption cycling tests showed that the adsorption efficiency of the composite was still over 95% after 3 cycles.
31284003	4	27	theme	thermogravimetric	694:710	arg1	analysis					712:719	thermogravimetric analysis	694:719	thermogravimetric analysis	694:719	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	1	28	theme	water	169:173	arg1	resources					175:183	the water resources	165:183	the water resources on which we depend	165:202	Due to the rapid development of industrialization, the water resources on which we depend are facing unprecedented challenges.
31284003	7	29	theme	π-π	1203:1205	arg1	effects					1207:1213	π-π effects	1203:1213	π-π effects	1203:1213	Investigation of the adsorption mechanism indicated that electrostatic forces and π-π effects are mainly involved in adsorption.
31284003	6	30	theme	Adsorption	971:980	arg1	tests					982:986	Adsorption tests	971:986	Adsorption tests	971:986	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	0	31	theme	graphite	44:51	arg1	oxide					53:57	graphite oxide	44:57	graphite oxide	44:57	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	5	32	theme	isotherm	856:863	arg1	measurements					865:876	Adsorption isotherm measurements	845:876	Adsorption isotherm measurements	845:876	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	7	33	theme	electrostatic	1178:1190	arg1	forces					1192:1197	electrostatic forces	1178:1197	electrostatic forces	1178:1197	Investigation of the adsorption mechanism indicated that electrostatic forces and π-π effects are mainly involved in adsorption.
31284003	3	34	theme	graft	504:508	arg1	polymerization					510:523	graft polymerization	504:523	graft polymerization	504:523	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	6	35	theme	maximum	1055:1061	arg1	capacity					1074:1081	the maximum adsorption capacity	1051:1081	the maximum adsorption capacity for ethyl violet dye	1051:1102	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	6	35	theme	maximum	1055:1061	arg1	273.99 mg/g					1108:1118	273.99 mg/g	1108:1118	273.99 mg/g	1108:1118	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	0	36	theme	nanocomposites	13:26	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.	0:112	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	4	37	theme	scanning	742:749	arg1	microscopy					760:769	scanning electron microscopy	742:769	scanning electron microscopy	742:769	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	4	38	theme	Fourier-transform	634:650	arg1	spectroscopy					661:672	Fourier-transform infrared spectroscopy	634:672	Fourier-transform infrared spectroscopy	634:672	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	2	39	theme	dyes	372:375	arg1	removal					361:367	the removal	357:367	the removal of dyes from wastewater	357:391	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	6	40	theme	adsorption	1063:1072	arg1	capacity					1074:1081	the maximum adsorption capacity	1051:1081	the maximum adsorption capacity for ethyl violet dye	1051:1102	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	6	40	theme	adsorption	1063:1072	arg1	273.99 mg/g					1108:1118	273.99 mg/g	1108:1118	273.99 mg/g	1108:1118	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	3	41	used	used	529:532	arg2	nanocomposite					474:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	6	42	theme	ethyl	1087:1091	arg1	dye					1100:1102	ethyl violet dye	1087:1102	ethyl violet dye	1087:1102	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	5	43	theme	Adsorption	845:854	arg1	measurements					865:876	Adsorption isotherm measurements	845:876	Adsorption isotherm measurements	845:876	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	0	44	from	remediation	63:73	arg1	solutions					103:111	aqueous solutions	95:111	aqueous solutions	95:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	3	45	theme	cationic	552:559	arg1	dye					574:576	cationic ethyl violet dye	552:576	cationic ethyl violet dye	552:576	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	7	46	theme	adsorption	1142:1151	arg1	mechanism					1153:1161	the adsorption mechanism	1138:1161	the adsorption mechanism	1138:1161	Investigation of the adsorption mechanism indicated that electrostatic forces and π-π effects are mainly involved in adsorption.
31284003	9	47	theme	potential	1460:1468	arg1	applications					1470:1481	potential applications	1460:1481	potential applications	1460:1481	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	8	48	theme	adsorption	1291:1300	arg1	efficiency					1302:1311	the adsorption efficiency	1287:1311	the adsorption efficiency of the composite	1287:1328	Desorption cycling tests showed that the adsorption efficiency of the composite was still over 95% after 3 cycles.
31284003	8	49	theme	composite	1320:1328	arg1	efficiency					1302:1311	the adsorption efficiency	1287:1311	the adsorption efficiency of the composite	1287:1328	Desorption cycling tests showed that the adsorption efficiency of the composite was still over 95% after 3 cycles.
31284003	2	50	theme	important	408:416	arg1	topic					418:422	an important topic	405:422	an important topic	405:422	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	4	51	used	used	815:818	arg2	techniques					616:625	Various techniques	608:625	Various techniques	608:625	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	0	52	from	solutions	103:111	arg1	remediation					63:73	remediation	63:73	remediation of cationic dyes in aqueous solutions	63:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	9	53	theme	dye	1495:1497	arg1	removal					1499:1505	cationic dye removal	1486:1505	cationic dye removal	1486:1505	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	3	54	theme	acrylic	445:451	arg1	xylan/poly					434:443	A porous xylan/poly	425:443	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	3	54	theme	acrylic	445:451	arg1	acid					453:456	acrylic acid	445:456	acrylic acid	445:456	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	9	55	theme	porous	1394:1399	arg1	xylan/poly					1401:1410	porous xylan/poly	1394:1410	this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite	1389:1454	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	9	55	theme	porous	1394:1399	arg1	acid					1421:1424	acrylic acid	1413:1424	acrylic acid	1413:1424	These results show that this porous xylan/poly (acrylic acid)/graphite oxide nanocomposite has potential applications in cationic dye removal.
31284003	7	56	theme	mechanism	1153:1161	arg1	Investigation					1121:1133	Investigation	1121:1133	Investigation of the adsorption mechanism	1121:1161	Investigation of the adsorption mechanism indicated that electrostatic forces and π-π effects are mainly involved in adsorption.
31284003	3	57	theme	dye	574:576	arg1	adsorption					538:547	adsorption	538:547	adsorption of cationic ethyl violet dye	538:576	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	4	58	theme	ultraviolet-visible	776:794	arg1	spectroscopy					796:807	ultraviolet-visible spectroscopy	776:807	ultraviolet-visible spectroscopy	776:807	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	2	59	theme	great	303:307	arg1	pollution					309:317	great pollution	303:317	great pollution to the water resources in nature	303:350	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	5	60	theme	Langmuir	935:942	arg1	adsorption					953:962	the Langmuir isotherm adsorption	931:962	the Langmuir isotherm adsorption model	931:968	Adsorption isotherm measurements showed that the composite's adsorption behavior fits the Langmuir isotherm adsorption model.
31284003	6	61	theme	adsorption	1028:1037	arg1	properties					1039:1048	excellent adsorption properties	1018:1048	excellent adsorption properties	1018:1048	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	4	62	theme	electron	751:758	arg1	microscopy					760:769	scanning electron microscopy	742:769	scanning electron microscopy	742:769	Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elemental analysis, scanning electron microscopy, and ultraviolet-visible spectroscopy, were used to study this composite.
31284003	3	63	theme	xylan/poly	434:443	arg1	nanocomposite					474:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	6	64	contain	has	1014:1016	arg2	properties					1039:1048	excellent adsorption properties	1018:1048	excellent adsorption properties	1018:1048	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	6	64	contain	has	1014:1016	arg1	material					1005:1012	this material	1000:1012	this material	1000:1012	Adsorption tests showed that this material has excellent adsorption properties; the maximum adsorption capacity for ethyl violet dye was 273.99 mg/g.
31284003	3	65	theme	porous	427:432	arg1	xylan/poly					434:443	A porous xylan/poly	425:443	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	3	65	theme	porous	427:432	arg1	acid					453:456	acrylic acid	445:456	acrylic acid	445:456	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	0	66	from	dyes	87:90	arg1	solutions					103:111	aqueous solutions	95:111	aqueous solutions	95:111	Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
31284003	3	67	theme	ethyl	561:565	arg1	dye					574:576	cationic ethyl violet dye	552:576	cationic ethyl violet dye	552:576	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	2	68	from	removal	361:367	arg1	wastewater					382:391	wastewater	382:391	wastewater	382:391	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	1	69	theme	rapid	125:129	arg1	development					131:141	the rapid development	121:141	the rapid development of industrialization	121:162	Due to the rapid development of industrialization, the water resources on which we depend are facing unprecedented challenges.
31284003	2	70	from	substance	267:275	arg1	lives					284:288	our lives	280:288	our lives	280:288	Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic.
31284003	1	71	theme	unprecedented	215:227	arg1	challenges					229:238	unprecedented challenges	215:238	unprecedented challenges	215:238	Due to the rapid development of industrialization, the water resources on which we depend are facing unprecedented challenges.
31284003	8	72	theme	Desorption	1250:1259	arg1	tests					1269:1273	Desorption cycling tests	1250:1273	Desorption cycling tests	1250:1273	Desorption cycling tests showed that the adsorption efficiency of the composite was still over 95% after 3 cycles.
31284003	3	73	theme	violet	567:572	arg1	dye					574:576	cationic ethyl violet dye	552:576	cationic ethyl violet dye	552:576	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
31284003	3	74	theme	oxide	468:472	arg1	nanocomposite					474:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite	425:486	A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper.
30557678	0	0	theme	oral	73:76	arg1	delivery					98:105	oral colon-specific drug delivery	73:105	oral colon-specific drug delivery	73:105	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	7	1	from	HAS/Zein	1046:1053	arg1	release					1014:1020	Drug release	1009:1020	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included)	1009:1161	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	2	theme	upper	1076:1080	arg1	conditions					1099:1108	upper gastrointestinal conditions	1076:1108	upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included)	1076:1161	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	9	3	theme	targeting	1726:1734	arg1	purposes					1736:1743	colonic targeting purposes	1718:1743	colonic targeting purposes	1718:1743	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	1	4	theme	free	148:151	arg1	films					153:157	free films	148:157	free films of zein with and without acetylated high amylose maize starch (HAS)	148:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	7	5	from	release	1317:1323	arg1	region					1333:1338	this region	1328:1338	this region	1328:1338	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	6	6	theme	compositions	979:990	arg1	coatings					957:964	HAS/Zein aqueous based coatings	934:964	HAS/Zein aqueous based coatings of different compositions to a TWG of 20%	934:1006	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	7	7	theme	gastrointestinal	1082:1097	arg1	conditions					1099:1108	upper gastrointestinal conditions	1076:1108	upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included)	1076:1161	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	4	8	from	differences	735:745	arg1	appearance					750:759	appearance	750:759	appearance within the same film	750:780	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	1	9	theme	films	153:157	arg1	approach					277:284	a novel approach	269:284	a novel approach to colonic drug delivery	269:309	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	1	9	theme	films	153:157	arg1	preparation					133:143	the preparation	129:143	the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets	129:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	6	10	theme	different	969:977	arg1	compositions					979:990	different compositions	969:990	different compositions	969:990	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	0	11	theme	drug	93:96	arg1	delivery					98:105	oral colon-specific drug delivery	73:105	oral colon-specific drug delivery	73:105	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	3	12	theme	zein	491:494	arg1	films					482:486	Free films	477:486	Free films of zein alone and starch/zein	477:516	Free films of zein alone and starch/zein were prepared and characterized.
30557678	4	13	theme	analysis	700:707	arg1	data					709:712	nanothermal analysis data	688:712	nanothermal analysis data	688:712	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	7	14	from	zein	1027:1030	arg1	release					1014:1020	Drug release	1009:1020	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included)	1009:1161	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	0	15	theme	colon-specific	78:91	arg1	delivery					98:105	oral colon-specific drug delivery	73:105	oral colon-specific drug delivery	73:105	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	4	16	theme	lower	656:660	arg1	ratios					671:676	lower HAS/Zein ratios	656:676	lower HAS/Zein ratios	656:676	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	6	17	theme	aqueous	943:949	arg1	coatings					957:964	HAS/Zein aqueous based coatings	934:964	HAS/Zein aqueous based coatings of different compositions to a TWG of 20%	934:1006	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	1	18	theme	corresponding	237:249	arg1	tablets					258:264	their corresponding coated tablets	231:264	their corresponding coated tablets	231:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	7	19	dep	conditions	1099:1108	arg1	pH					1111:1112	pH 1.2	1111:1116	pH 1.2	1111:1116	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	19	dep	conditions	1099:1108	arg1	pH					1119:1120	pH 6.8	1119:1124	pH 6.8 with pepsin and pancreatin included	1119:1160	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	4	20	theme	film	573:576	arg1	surface					578:584	film surface	573:584	film surface	573:584	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	8	21	theme	drug	1520:1523	arg1	rapid					1567:1571	rapid	1567:1571	rapid	1567:1571	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	21	theme	drug	1520:1523	arg1	release					1525:1531	the drug release	1516:1531	the drug release	1516:1531	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	21	theme	drug	1520:1523	arg1	compared					1592:1599	compared	1592:1599	compared to the zein alone coating formulation	1592:1637	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	2	22	theme	starch	362:367	arg1	component					369:377	a digestible starch component	349:377	a digestible starch component within the inert zein	349:399	We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached.
30557678	8	23	theme	simulated	1486:1494	arg1	conditions					1504:1513	simulated colonic conditions	1486:1513	simulated colonic conditions	1486:1513	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	4	24	theme	compositional	790:802	arg1	differences					804:814	compositional differences	790:814	compositional differences	790:814	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	4	24	theme	compositional	790:802	arg1	differences					735:745	these differences	729:745	these differences in appearance within the same film	729:780	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	7	25	theme	drug	1226:1229	arg1	%					1209:1209	approximately 12%	1193:1209	approximately 12%	1193:1209	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	25	theme	drug	1226:1229	arg1	%					1217:1217	14%	1215:1217	14% of the drug	1215:1229	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	25	theme	drug	1226:1229	arg1	drug					1226:1229	the drug	1222:1229	the drug	1222:1229	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	26	from	6 h	1265:1267	arg1	test					1294:1297	a sequential in vitro test	1272:1297	a sequential in vitro test	1272:1297	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	1	27	theme	zein	162:165	arg1	tablets					258:264	their corresponding coated tablets	231:264	their corresponding coated tablets	231:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	1	27	theme	zein	162:165	arg1	films					153:157	free films	148:157	free films of zein with and without acetylated high amylose maize starch (HAS)	148:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	6	28	theme	based	951:955	arg1	coatings					957:964	HAS/Zein aqueous based coatings	934:964	HAS/Zein aqueous based coatings of different compositions to a TWG of 20%	934:1006	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	4	29	theme	nanothermal	688:698	arg1	data					709:712	nanothermal analysis data	688:712	nanothermal analysis data	688:712	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	7	30	dep	in	1285:1286	arg1	vitro					1288:1292	vitro	1288:1292	vitro	1288:1292	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	0	31	theme	In	0:1	arg1	release					14:20	In vitro drug release	0:20	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.	0:106	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	1	32	theme	coated	251:256	arg1	tablets					258:264	their corresponding coated tablets	231:264	their corresponding coated tablets	231:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	6	33	theme	Paracetamol	897:907	arg1	tablets					909:915	Paracetamol tablets	897:915	Paracetamol tablets	897:915	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	7	34	with	pH	1119:1120	arg1	pancreatin					1142:1151	pancreatin	1142:1151	pancreatin	1142:1151	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	34	with	pH	1119:1120	arg1	pepsin					1131:1136	pepsin	1131:1136	pepsin	1131:1136	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	9	35	theme	potential	1673:1681	arg1	use					1683:1685	the potential use	1669:1685	the potential use of zein-starch mixed films for colonic targeting purposes	1669:1743	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	1	36	theme	tablets	258:264	arg1	approach					277:284	a novel approach	269:284	a novel approach to colonic drug delivery	269:309	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	1	36	theme	tablets	258:264	arg1	preparation					133:143	the preparation	129:143	the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets	129:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	9	37	theme	zein-starch	1690:1700	arg1	films					1708:1712	zein-starch mixed films	1690:1712	zein-starch mixed films	1690:1712	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	7	38	theme	coated	1055:1060	arg1	tablets					1062:1068	coated tablets	1055:1068	coated tablets	1055:1068	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	2	39	theme	inert	390:394	arg1	zein					396:399	the inert zein	386:399	the inert zein	386:399	We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached.
30557678	0	40	theme	drug	9:12	arg1	release					14:20	In vitro drug release	0:20	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.	0:106	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	6	41	theme	HAS/Zein	934:941	arg1	coatings					957:964	HAS/Zein aqueous based coatings	934:964	HAS/Zein aqueous based coatings of different compositions to a TWG of 20%	934:1006	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	4	42	theme	AFM	559:561	arg1	images					563:568	AFM images	559:568	AFM images	559:568	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	8	43	dep	in	1447:1448	arg1	vitro					1450:1454	vitro	1450:1454	vitro	1450:1454	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	3	44	theme	starch/zein	506:516	arg1	films					482:486	Free films	477:486	Free films of zein alone and starch/zein	477:516	Free films of zein alone and starch/zein were prepared and characterized.
30557678	1	45	theme	acetylated	184:193	arg1	starch					214:219	acetylated high amylose maize starch	184:219	acetylated high amylose maize starch (HAS)	184:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	0	46	theme	acetylated	27:36	arg1	films					63:67	acetylated high amylose starch-zein films	27:67	acetylated high amylose starch-zein films	27:67	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	6	47	theme	%	1006:1006	arg1	TWG					997:999	a TWG	995:999	a TWG of 20%	995:1006	Paracetamol tablets were coated with HAS/Zein aqueous based coatings of different compositions to a TWG of 20%.
30557678	7	48	theme	in	1285:1286	arg1	test					1294:1297	a sequential in vitro test	1272:1297	a sequential in vitro test	1272:1297	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	9	49	theme	colonic	1718:1724	arg1	purposes					1736:1743	colonic targeting purposes	1718:1743	colonic targeting purposes	1718:1743	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	1	50	theme	high	195:198	arg1	starch					214:219	acetylated high amylose maize starch	184:219	acetylated high amylose maize starch (HAS)	184:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	1	51	theme	novel	271:275	arg1	approach					277:284	a novel approach	269:284	a novel approach to colonic drug delivery	269:309	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	1	51	theme	novel	271:275	arg1	preparation					133:143	the preparation	129:143	the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets	129:264	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	0	52	from	films	63:67	arg1	release					14:20	In vitro drug release	0:20	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.	0:106	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	4	53	theme	same	772:775	arg1	film					777:780	the same film	768:780	the same film	768:780	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	1	54	theme	amylose	200:206	arg1	starch					214:219	acetylated high amylose maize starch	184:219	acetylated high amylose maize starch (HAS)	184:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	0	55	theme	amylose	43:49	arg1	films					63:67	acetylated high amylose starch-zein films	27:67	acetylated high amylose starch-zein films	27:67	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	1	56	dep	starch	214:219	arg1	HAS					222:224	HAS	222:224	HAS	222:224	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	8	57	dep	significantly	1537:1549	arg1	p < 0.05					1552:1559	p < 0.05	1552:1559	p < 0.05	1552:1559	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	0	58	theme	high	38:41	arg1	films					63:67	acetylated high amylose starch-zein films	27:67	acetylated high amylose starch-zein films	27:67	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	8	59	theme	in	1447:1448	arg1	model					1462:1466	an in vitro colon model	1444:1466	an in vitro colon model	1444:1466	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	7	60	theme	Drug	1009:1012	arg1	release					1014:1020	Drug release	1009:1020	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included)	1009:1161	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	7	61	theme	sequential	1274:1283	arg1	test					1294:1297	a sequential in vitro test	1272:1297	a sequential in vitro test	1272:1297	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	4	62	theme	HAS/Zein	662:669	arg1	ratios					671:676	lower HAS/Zein ratios	656:676	lower HAS/Zein ratios	656:676	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	9	63	theme	mixed	1702:1706	arg1	films					1708:1712	zein-starch mixed films	1690:1712	zein-starch mixed films	1690:1712	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	1	64	theme	colonic	289:295	arg1	delivery					302:309	colonic drug delivery	289:309	colonic drug delivery	289:309	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	8	65	dep	rapid	1567:1571	arg1	rapid					1567:1571	rapid	1567:1571	rapid	1567:1571	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	65	dep	rapid	1567:1571	arg1	release					1525:1531	the drug release	1516:1531	the drug release	1516:1531	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	65	dep	rapid	1567:1571	arg1	compared					1592:1599	compared	1592:1599	compared to the zein alone coating formulation	1592:1637	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	66	theme	colon	1456:1460	arg1	model					1462:1466	an in vitro colon model	1444:1466	an in vitro colon model	1444:1466	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	9	67	theme	films	1708:1712	arg1	use					1683:1685	the potential use	1669:1685	the potential use of zein-starch mixed films for colonic targeting purposes	1669:1743	These data therefore support the potential use of zein-starch mixed films for colonic targeting purposes.
30557678	1	68	theme	maize	208:212	arg1	starch					214:219	acetylated high amylose maize starch	184:219	acetylated high amylose maize starch (HAS)	184:225	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	7	69	dep	zein	1027:1030	arg1	tablets					1062:1068	coated tablets	1055:1068	coated tablets	1055:1068	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	2	70	theme	large	449:453	arg1	intestine					455:463	the large intestine	445:463	the large intestine	445:463	We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached.
30557678	4	71	from	ratios	671:676	arg1	inhomogeneous					625:637	inhomogeneous	625:637	inhomogeneous	625:637	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	7	72	theme	1:5	1042:1044	arg1	HAS/Zein					1046:1053	1:5 HAS/Zein	1042:1053	1:5 HAS/Zein	1042:1053	Drug release from zein alone and 1:5 HAS/Zein coated tablets under upper gastrointestinal conditions (pH 1.2, pH 6.8 with pepsin and pancreatin included) was very similar (for example approximately 12% and 14% of the drug was released, respectively, after 6 h in a sequential in vitro test), suggesting that release in this region is limited and is not influenced by the presence of HAS in the ratio to zein under study.
30557678	2	73	theme	component	369:377	arg1	embedding					336:344	the embedding	332:344	the embedding of a digestible starch component within the inert zein	332:399	We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached.
30557678	8	74	from	1:5 HAS/Zein	1578:1589	arg1	rapid					1567:1571	rapid	1567:1571	rapid	1567:1571	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	74	from	1:5 HAS/Zein	1578:1589	arg1	release					1525:1531	the drug release	1516:1531	the drug release	1516:1531	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	8	74	from	1:5 HAS/Zein	1578:1589	arg1	compared					1592:1599	compared	1592:1599	compared to the zein alone coating formulation	1592:1637	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
30557678	4	75	theme	surface	578:584	arg1	SEM					551:553	SEM	551:553	SEM	551:553	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	4	75	theme	surface	578:584	arg1	images					563:568	AFM images	559:568	AFM images	559:568	SEM and AFM images of film surface showed that films were morphologically inhomogeneous, particularly at lower HAS/Zein ratios; however, nanothermal analysis data suggested that these differences in appearance within the same film are not compositional differences.
30557678	5	76	theme	molecular	849:857	arg1	interaction					859:869	no molecular interaction	846:869	no molecular interaction between the two polymers	846:894	Moreover, FT-IR could detect no molecular interaction between the two polymers.
30557678	2	77	theme	digestible	351:360	arg1	starch					362:367	a digestible starch	349:367	a digestible starch component within the inert zein	349:399	We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached.
30557678	0	78	theme	starch-zein	51:61	arg1	films					63:67	acetylated high amylose starch-zein films	27:67	acetylated high amylose starch-zein films	27:67	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	1	79	theme	drug	297:300	arg1	delivery					302:309	colonic drug delivery	289:309	colonic drug delivery	289:309	This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery.
30557678	0	80	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro drug release from acetylated high amylose starch-zein films for oral colon-specific drug delivery.
30557678	3	81	theme	Free	477:480	arg1	films					482:486	Free films	477:486	Free films of zein alone and starch/zein	477:516	Free films of zein alone and starch/zein were prepared and characterized.
30557678	8	82	theme	colonic	1496:1502	arg1	conditions					1504:1513	simulated colonic conditions	1486:1513	simulated colonic conditions	1486:1513	Studies using an in vitro colon model showed that under simulated colonic conditions, the drug release was significantly (p < 0.05) more rapid from 1:5 HAS/Zein, compared to the zein alone coating formulation.
31164685	12	0	theme	bulgaricus	1170:1179	arg1	B3					1181:1182	bulgaricus B3	1170:1182	bulgaricus B3	1170:1182	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	0	1	theme	ability	84:90	arg1	characteristics					40:54	the structural characteristics	25:54	the structural characteristics of lactobacilli-EPS and its ability	25:90	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	4	2	theme	flow	468:471	arg1	cytometry					473:481	flow cytometry	468:481	flow cytometry	468:481	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	14	3	dep	mannose	1477:1483	arg1	composition					1497:1507	composition	1497:1507	composition	1497:1507	Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.
31164685	3	4	dep	EPSs	356:359	arg1	study					337:341	this study	332:341	this study	332:341	In this study, lyophilized EPSs of four Lactobacillus spp.
31164685	6	5	theme	Western	749:755	arg1	Blotting					757:764	Western Blotting	749:764	Western Blotting	749:764	Changes in apoptotic-markers were examined by qPCR and Western Blotting.
31164685	7	6	theme	time-dependent	818:831	arg1	manner					833:838	a time-dependent manner	816:838	a time-dependent manner	816:838	EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin.
31164685	11	7	theme	ssp	1165:1167	arg1	EPS					1143:1145	EPS	1143:1145	EPS of L. delbrueckii ssp	1143:1167	EPS of L. delbrueckii ssp.
31164685	5	8	theme	monosaccharide	572:585	arg1	composition					587:597	their monosaccharide composition	566:597	their monosaccharide composition	566:597	The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively.
31164685	2	9	theme	potential	262:270	arg1	prebiotics					272:281	potential prebiotics	262:281	potential prebiotics	262:281	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect.
31164685	0	10	theme	colon	115:119	arg1	cells					128:132	colon cancer cells	115:132	colon cancer cells	115:132	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	9	11	contain	contained	1056:1064	arg2	arabinose					1066:1074	arabinose	1066:1074	arabinose	1066:1074	Some contained arabinose or fructose.
31164685	9	11	contain	contained	1056:1064	arg1	Some					1051:1054	Some	1051:1054	Some	1051:1054	Some contained arabinose or fructose.
31164685	9	11	contain	contained	1056:1064	arg2	fructose					1079:1086	fructose	1079:1086	fructose	1079:1086	Some contained arabinose or fructose.
31164685	7	12	theme	Caspase	900:906	arg1	expression					881:890	the expression	877:890	the expression of Bax, Caspase 3 and 9	877:914	EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin.
31164685	8	13	with	mannose	972:978	arg1	proportions					1038:1048	different relative proportions	1019:1048	different relative proportions	1019:1048	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	12	14	theme	lowest	1229:1234	arg1	mannose					1213:1219	mannose	1213:1219	mannose	1213:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	14	theme	lowest	1229:1234	arg1	glucose					1246:1252	glucose	1246:1252	glucose	1246:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	14	theme	lowest	1229:1234	arg1	amount					1236:1241	the lowest amount	1225:1241	the lowest amount of glucose	1225:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	5	15	theme	molecular	600:608	arg1	weight					610:615	molecular weight	600:615	molecular weight (MW)	600:620	The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively.
31164685	5	15	theme	molecular	600:608	arg1	MW					618:619	MW	618:619	MW	618:619	The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively.
31164685	5	16	theme	lactobacilli-EPS	525:540	arg1	capability					509:518	capability	509:518	capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type	509:638	The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively.
31164685	7	17	theme	Bax	895:897	arg1	expression					881:890	the expression	877:890	the expression of Bax, Caspase 3 and 9	877:914	EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin.
31164685	11	18	theme	L.	1150:1151	arg1	ssp					1165:1167	L. delbrueckii ssp	1150:1167	L. delbrueckii ssp	1150:1167	EPS of L. delbrueckii ssp.
31164685	8	19	with	N-acetylglucosamine	994:1012	arg1	proportions					1038:1048	different relative proportions	1019:1048	different relative proportions	1019:1048	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	13	20	theme	cell	1336:1339	arg1	proliferation					1341:1353	cell proliferation	1336:1353	cell proliferation	1336:1353	In conclusion, lactobacilli-EPSs inhibit cell proliferation in HT-29 via apoptosis.
31164685	8	21	theme	different	1019:1027	arg1	proportions					1038:1048	different relative proportions	1019:1048	different relative proportions	1019:1048	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	1	22	theme	common	176:181	arg1	cancer					183:188	the most common cancer	167:188	the most common cancer around the world	167:205	Colon cancer is one of the most common cancer around the world.
31164685	3	23	theme	lyophilized	344:354	arg1	EPSs					356:359	lyophilized EPSs	344:359	lyophilized EPSs of four Lactobacillus spp	344:385	In this study, lyophilized EPSs of four Lactobacillus spp.
31164685	0	24	theme	cancer	121:126	arg1	cells					128:132	colon cancer cells	115:132	colon cancer cells	115:132	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	1	25	theme	cancer	183:188	arg1	cancer					183:188	the most common cancer	167:188	the most common cancer around the world	167:205	Colon cancer is one of the most common cancer around the world.
31164685	1	25	theme	cancer	183:188	arg1	one					160:162	one	160:162	one	160:162	Colon cancer is one of the most common cancer around the world.
31164685	3	26	theme	spp	383:385	arg1	EPSs					356:359	lyophilized EPSs	344:359	lyophilized EPSs of four Lactobacillus spp	344:385	In this study, lyophilized EPSs of four Lactobacillus spp.
31164685	11	27	theme	delbrueckii	1153:1163	arg1	ssp					1165:1167	L. delbrueckii ssp	1150:1167	L. delbrueckii ssp	1150:1167	EPS of L. delbrueckii ssp.
31164685	12	28	theme	apoptosis	1274:1282	arg1	induction					1284:1292	the highest apoptosis induction	1262:1292	the highest apoptosis induction	1262:1292	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	8	29	with	glucose	981:987	arg1	proportions					1038:1048	different relative proportions	1019:1048	different relative proportions	1019:1048	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	0	30	theme	structural	29:38	arg1	characteristics					40:54	the structural characteristics	25:54	the structural characteristics of lactobacilli-EPS and its ability	25:90	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	4	31	from	apoptosis	408:416	arg1	HT-29					441:445	HT-29	441:445	HT-29	441:445	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	4	31	from	apoptosis	408:416	arg1	cells					434:438	colon cancer cells	421:438	colon cancer cells (HT-29)	421:446	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	4	32	theme	cancer	427:432	arg1	HT-29					441:445	HT-29	441:445	HT-29	441:445	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	4	32	theme	cancer	427:432	arg1	cells					434:438	colon cancer cells	421:438	colon cancer cells (HT-29)	421:446	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	12	33	theme	highest	1266:1272	arg1	induction					1284:1292	the highest apoptosis induction	1262:1292	the highest apoptosis induction	1262:1292	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	8	34	contain	contained	962:970	arg2	N-acetylglucosamine					994:1012	N-acetylglucosamine	994:1012	N-acetylglucosamine	994:1012	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	8	34	contain	contained	962:970	arg1	EPSs					957:960	All EPSs	953:960	All EPSs	953:960	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	8	34	contain	contained	962:970	arg2	glucose					981:987	glucose	981:987	glucose	981:987	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	8	34	contain	contained	962:970	arg2	mannose					972:978	mannose	972:978	mannose	972:978	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31164685	0	35	theme	lactobacilli-EPS	59:74	arg1	characteristics					40:54	the structural characteristics	25:54	the structural characteristics of lactobacilli-EPS and its ability	25:90	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	5	36	theme	linkage	627:633	arg1	type					635:638	linkage type	627:638	linkage type	627:638	The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively.
31164685	4	37	theme	colon	421:425	arg1	HT-29					441:445	HT-29	441:445	HT-29	441:445	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	4	37	theme	colon	421:425	arg1	cells					434:438	colon cancer cells	421:438	colon cancer cells (HT-29)	421:446	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	3	38	theme	Lactobacillus	369:381	arg1	spp					383:385	four Lactobacillus spp	364:385	four Lactobacillus spp	364:385	In this study, lyophilized EPSs of four Lactobacillus spp.
31164685	4	39	from	impact	398:403	arg1	apoptosis					408:416	apoptosis	408:416	apoptosis in colon cancer cells (HT-29)	408:446	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	2	40	theme	anti-tumor	310:319	arg1	effect					321:326	an anti-tumor effect	307:326	an anti-tumor effect	307:326	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect.
31164685	4	41	theme	for	388:390	arg1	impact					398:403	their impact	392:403	for their impact on apoptosis in colon cancer cells (HT-29)	388:446	for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry.
31164685	12	42	theme	highest	1195:1201	arg1	mannose					1213:1219	mannose	1213:1219	mannose	1213:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	42	theme	highest	1195:1201	arg1	amount					1203:1208	the highest amount	1191:1208	the highest amount of mannose	1191:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	42	theme	highest	1195:1201	arg1	glucose					1246:1252	glucose	1246:1252	glucose	1246:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	14	43	theme	EPS	1445:1447	arg1	glucose					1489:1495	glucose	1489:1495	glucose	1489:1495	Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.
31164685	14	43	theme	EPS	1445:1447	arg1	mannose					1477:1483	mannose	1477:1483	mannose	1477:1483	Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.
31164685	14	43	theme	EPS	1445:1447	arg1	ability					1434:1440	the ability	1430:1440	the ability of EPS to induce apoptosis	1430:1467	Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.
31164685	12	44	contain	having	1184:1189	arg2	amount					1203:1208	the highest amount	1191:1208	the highest amount of mannose	1191:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	44	contain	having	1184:1189	arg2	amount					1236:1241	the lowest amount	1225:1241	the lowest amount of glucose	1225:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	44	contain	having	1184:1189	arg2	glucose					1246:1252	glucose	1246:1252	glucose	1246:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	44	contain	having	1184:1189	arg1	B3					1181:1182	bulgaricus B3	1170:1182	bulgaricus B3	1170:1182	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	44	contain	having	1184:1189	arg2	mannose					1213:1219	mannose	1213:1219	mannose	1213:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	0	45	from	apoptosis	102:110	arg1	cells					128:132	colon cancer cells	115:132	colon cancer cells	115:132	The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro.
31164685	12	46	theme	glucose	1246:1252	arg1	mannose					1213:1219	mannose	1213:1219	mannose	1213:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	46	theme	glucose	1246:1252	arg1	amount					1203:1208	the highest amount	1191:1208	the highest amount of mannose	1191:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	46	theme	glucose	1246:1252	arg1	amount					1236:1241	the lowest amount	1225:1241	the lowest amount of glucose	1225:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	46	theme	glucose	1246:1252	arg1	glucose					1246:1252	glucose	1246:1252	glucose	1246:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	6	47	from	Changes	694:700	arg1	apoptotic-markers					705:721	apoptotic-markers	705:721	apoptotic-markers	705:721	Changes in apoptotic-markers were examined by qPCR and Western Blotting.
31164685	12	48	theme	mannose	1213:1219	arg1	mannose					1213:1219	mannose	1213:1219	mannose	1213:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	48	theme	mannose	1213:1219	arg1	amount					1203:1208	the highest amount	1191:1208	the highest amount of mannose	1191:1219	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	48	theme	mannose	1213:1219	arg1	amount					1236:1241	the lowest amount	1225:1241	the lowest amount of glucose	1225:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	12	48	theme	mannose	1213:1219	arg1	glucose					1246:1252	glucose	1246:1252	glucose	1246:1252	bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction.
31164685	2	49	contain	have	302:305	arg1	Exopolysaccharides					208:225	Exopolysaccharides	208:225	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics	208:281	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect.
31164685	2	49	contain	have	302:305	arg2	effect					321:326	an anti-tumor effect	307:326	an anti-tumor effect	307:326	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect.
31164685	2	49	contain	have	302:305	arg1	EPSs					228:231	EPSs	228:231	EPSs	228:231	Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect.
31164685	7	50	theme	9	914:914	arg1	expression					881:890	the expression	877:890	the expression of Bax, Caspase 3 and 9	877:914	EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin.
31164685	1	51	theme	Colon	144:148	arg1	cancer					150:155	Colon cancer	144:155	Colon cancer	144:155	Colon cancer is one of the most common cancer around the world.
31164685	8	52	theme	relative	1029:1036	arg1	proportions					1038:1048	different relative proportions	1019:1048	different relative proportions	1019:1048	All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions.
31475377	10	0	dep	food	1689:1692	arg1	industry					1702:1709	industry	1702:1709	industry	1702:1709	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	10	0	dep	food	1689:1692	arg1	the					1685:1687	the	1685:1687	the	1685:1687	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	10	1	theme	structural	1586:1595	arg1	features					1597:1604	structural features	1586:1604	structural features	1586:1604	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	2	2	theme	flaxseeds	313:321	arg1	varieties					300:308	golden varieties	293:308	golden varieties of flaxseeds	293:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	2	2	theme	flaxseeds	313:321	arg1	flaxseeds					313:321	flaxseeds	313:321	flaxseeds	313:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	2	2	theme	flaxseeds	313:321	arg1	brown					283:287	brown	283:287	brown	283:287	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	0	3	theme	potential	86:94	arg1	activity					108:115	its potential antioxidant activity	82:115	its potential antioxidant activity	82:115	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	3	4	theme	important	544:552	arg1	polysaccharide					554:567	this important polysaccharide	539:567	this important polysaccharide	539:567	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	7	5	dep	resonance	1207:1215	arg1	H					1226:1226	1 H	1224:1226	1 H	1224:1226	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	5	dep	resonance	1207:1215	arg1	C					1232:1232	13 C	1229:1232	13 C	1229:1232	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	8	6	theme	Flaxseed	1339:1346	arg1	polymar					1378:1384	a natural carbohydrate polymar	1355:1384	a natural carbohydrate polymar	1355:1384	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	8	6	theme	Flaxseed	1339:1346	arg1	gum					1348:1350	Flaxseed gum	1339:1350	Flaxseed gum	1339:1350	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	1	7	theme	seed	238:241	arg1	mass					243:246	seed mass	238:246	seed mass	238:246	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	5	8	theme	liquid	706:711	arg1	chromatography					713:726	High-performance liquid chromatography	689:726	High-performance liquid chromatography (HPLC) screening	689:743	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	5	8	theme	liquid	706:711	arg1	HPLC					729:732	HPLC	729:732	HPLC	729:732	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	10	9	theme	practical	1660:1668	arg1	application					1670:1680	the practical application	1656:1680	the practical application in the food or drug industry	1656:1709	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	1	10	theme	mass	243:246	arg1	%					233:233	approximately 8%	218:233	approximately 8% of seed mass	218:246	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	1	10	theme	mass	243:246	arg1	mass					243:246	seed mass	238:246	seed mass	238:246	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	5	11	theme	chromatography	713:726	arg1	screening					735:743	High-performance liquid chromatography (HPLC) screening	689:743	High-performance liquid chromatography (HPLC) screening	689:743	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	6	12	theme	total	1055:1059	arg1	activity					1073:1080	total antioxidant activity	1055:1080	total antioxidant activity	1055:1080	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	1	13	theme	acidic	186:191	arg1	components					193:202	neutral and acidic components	174:202	neutral and acidic components	174:202	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	6	14	theme	antioxidant	1107:1117	arg1	potency					1119:1125	the antioxidant potency	1103:1125	the antioxidant potency of FSG	1103:1132	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	5	15	theme	glucuronic	869:878	arg1	arabinose					800:808	arabinose	800:808	arabinose	800:808	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	5	15	theme	glucuronic	869:878	arg1	acid					880:883	glucuronic acid	869:883	glucuronic acid	869:883	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	4	16	theme	polydispersity	632:645	arg1	ratio					647:651	a polydispersity ratio	630:651	a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz	630:686	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	0	17	theme	antioxidant	96:106	arg1	activity					108:115	its potential antioxidant activity	82:115	its potential antioxidant activity	82:115	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	7	18	theme	Fourier-transform	1135:1151	arg1	FTIR					1176:1179	FTIR	1176:1179	FTIR	1176:1179	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	18	theme	Fourier-transform	1135:1151	arg1	spectroscopy					1162:1173	Fourier-transform infrared spectroscopy	1135:1173	Fourier-transform infrared spectroscopy (FTIR)	1135:1180	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	10	19	theme	bioactive	1563:1571	arg1	composition					1573:1583	bioactive composition	1563:1583	bioactive composition	1563:1583	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	3	20	theme	chemical	415:422	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	10	21	theme	gum	1531:1533	arg1	availability					1513:1524	The availability	1509:1524	The availability of a gum	1509:1533	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	2	22	theme	brown	283:287	arg1	mixture					272:278	mixture	272:278	mixture of brown and golden varieties of flaxseeds	272:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	3	23	theme	monosaccharide	390:403	arg1	analysis					405:412	monosaccharide analysis	390:412	monosaccharide analysis	390:412	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	0	24	theme	Flaxseed	0:7	arg1	gum					9:11	Flaxseed gum	0:11	Flaxseed gum	0:11	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	10	25	from	application	1670:1680	arg1	food					1689:1692	food	1689:1692	food	1689:1692	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	10	25	from	application	1670:1680	arg1	drug					1697:1700	drug	1697:1700	drug	1697:1700	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	9	26	theme	FSG	1504:1506	arg1	potential					1448:1456	antioxidant potential	1436:1456	antioxidant potential	1436:1456	This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG.
31475377	9	26	theme	FSG	1504:1506	arg1	characterization					1484:1499	structural characterization	1473:1499	structural characterization of FSG	1473:1506	This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG.
31475377	2	27	theme	extraction	336:345	arg1	method					347:352	hot water extraction method	326:352	hot water extraction method	326:352	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	3	28	theme	better	514:519	arg1	insight					521:527	a better insight	512:527	a better insight regarding this important polysaccharide	512:567	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	10	29	theme	antioxidant	1611:1621	arg1	potential					1623:1631	antioxidant potential	1611:1631	antioxidant potential	1611:1631	The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.
31475377	9	30	theme	structural	1473:1482	arg1	characterization					1484:1499	structural characterization	1473:1499	structural characterization of FSG	1473:1506	This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG.
31475377	6	31	theme	antioxidant	1061:1071	arg1	activity					1073:1080	total antioxidant activity	1055:1080	total antioxidant activity	1055:1080	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	3	32	theme	surface	441:447	arg1	morphology					449:458	surface morphology	441:458	surface morphology	441:458	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	0	33	theme	bioactive	26:34	arg1	composition					36:46	bioactive composition	26:46	bioactive composition	26:46	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	2	34	theme	varieties	300:308	arg1	mixture					272:278	mixture	272:278	mixture of brown and golden varieties of flaxseeds	272:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	9	35	theme	useful	1407:1412	arg1	information					1414:1424	useful information	1407:1424	useful information regarding antioxidant potential, chemical, and structural characterization of FSG	1407:1506	This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG.
31475377	7	36	theme	Nuclear	1190:1196	arg1	NMR					1218:1220	NMR	1218:1220	NMR	1218:1220	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	36	theme	Nuclear	1190:1196	arg1	resonance					1207:1215	Nuclear magnetic resonance	1190:1215	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	37	theme	groups	1280:1285	arg1	presence					1257:1264	the presence	1253:1264	the presence of functional groups typical for polysaccharide	1253:1312	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	1	38	theme	neutral	174:180	arg1	components					193:202	neutral and acidic components	174:202	neutral and acidic components	174:202	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	6	39	theme	antioxidant	981:991	arg1	activities					993:1002	The antioxidant activities	977:1002	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity	977:1080	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	5	40	theme	extracted	759:767	arg1	FSG					769:771	extracted FSG	759:771	extracted FSG	759:771	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	8	41	theme	carbohydrate	1365:1376	arg1	polymar					1378:1384	a natural carbohydrate polymar	1355:1384	a natural carbohydrate polymar	1355:1384	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	8	41	theme	carbohydrate	1365:1376	arg1	gum					1348:1350	Flaxseed gum	1339:1350	Flaxseed gum	1339:1350	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	7	42	theme	typical	1287:1293	arg1	groups					1280:1285	functional groups	1269:1285	functional groups typical for polysaccharide	1269:1312	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	3	43	theme	molecular	359:367	arg1	distribution					376:387	The molecular weight distribution	355:387	The molecular weight distribution	355:387	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	5	44	theme	High-performance	689:704	arg1	chromatography					713:726	High-performance liquid chromatography	689:726	High-performance liquid chromatography (HPLC) screening	689:743	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	5	44	theme	High-performance	689:704	arg1	HPLC					729:732	HPLC	729:732	HPLC	729:732	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	8	45	theme	natural	1357:1363	arg1	polymar					1378:1384	a natural carbohydrate polymar	1355:1384	a natural carbohydrate polymar	1355:1384	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	8	45	theme	natural	1357:1363	arg1	gum					1348:1350	Flaxseed gum	1339:1350	Flaxseed gum	1339:1350	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	1	46	dep	heteropolysaccharide	142:161	arg1	consist					163:169	consist	163:169	consist of neutral and acidic components	163:202	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	6	47	theme	reducing	1035:1042	arg1	power					1044:1048	reducing power	1035:1048	reducing power	1035:1048	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	3	48	theme	FSG	463:465	arg1	morphology					449:458	surface morphology	441:458	surface morphology	441:458	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	3	48	theme	FSG	463:465	arg1	distribution					376:387	The molecular weight distribution	355:387	The molecular weight distribution	355:387	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	3	48	theme	FSG	463:465	arg1	analysis					405:412	monosaccharide analysis	390:412	monosaccharide analysis	390:412	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	3	48	theme	FSG	463:465	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	3	49	theme	weight	369:374	arg1	distribution					376:387	The molecular weight distribution	355:387	The molecular weight distribution	355:387	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	8	50	theme	PRACTICAL	1315:1323	arg1	APPLICATIONS					1325:1336	PRACTICAL APPLICATIONS	1315:1336	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.	1315:1385	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	7	51	theme	functional	1269:1278	arg1	groups					1280:1285	functional groups	1269:1285	functional groups typical for polysaccharide	1269:1312	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	52	theme	infrared	1153:1160	arg1	FTIR					1176:1179	FTIR	1176:1179	FTIR	1176:1179	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	52	theme	infrared	1153:1160	arg1	spectroscopy					1162:1173	Fourier-transform infrared spectroscopy	1135:1173	Fourier-transform infrared spectroscopy (FTIR)	1135:1180	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	1	53	theme	Flaxseed	118:125	arg1	gum					127:129	Flaxseed gum	118:129	Flaxseed gum (FSG)	118:135	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	1	53	theme	Flaxseed	118:125	arg1	heteropolysaccharide					142:161	a heteropolysaccharide	140:161	a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass	140:246	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	1	53	theme	Flaxseed	118:125	arg1	FSG					132:134	FSG	132:134	FSG	132:134	Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass.
31475377	7	54	theme	magnetic	1198:1205	arg1	NMR					1218:1220	NMR	1218:1220	NMR	1218:1220	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	7	54	theme	magnetic	1198:1205	arg1	resonance					1207:1215	Nuclear magnetic resonance	1190:1215	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	0	55	dep	Extraction	14:23	arg1	gum					9:11	Flaxseed gum	0:11	Flaxseed gum	0:11	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	4	56	theme	1.6	656:658	arg1	ratio					647:651	a polydispersity ratio	630:651	a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz	630:686	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	2	57	theme	golden	293:298	arg1	varieties					300:308	golden varieties	293:308	golden varieties of flaxseeds	293:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	2	57	theme	golden	293:298	arg1	flaxseeds					313:321	flaxseeds	313:321	flaxseeds	313:321	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	5	58	theme	galacturonic	890:901	arg1	arabinose					800:808	arabinose	800:808	arabinose	800:808	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	5	58	theme	galacturonic	890:901	arg1	acid					903:906	galacturonic acid	890:906	galacturonic acid	890:906	High-performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously.
31475377	4	59	theme	molecular	582:590	arg1	1,322 kDa					615:623	1,322 kDa	615:623	1,322 kDa	615:623	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	4	59	theme	molecular	582:590	arg1	weight					592:597	The average molecular weight	570:597	The average molecular weight	570:597	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	8	60	dep	APPLICATIONS	1325:1336	arg1	polymar					1378:1384	a natural carbohydrate polymar	1355:1384	a natural carbohydrate polymar	1355:1384	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	8	60	dep	APPLICATIONS	1325:1336	arg1	gum					1348:1350	Flaxseed gum	1339:1350	Flaxseed gum	1339:1350	PRACTICAL APPLICATIONS: Flaxseed gum is a natural carbohydrate polymar.
31475377	6	61	theme	FSG	1007:1009	arg1	activities					993:1002	The antioxidant activities	977:1002	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity	977:1080	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	3	62	theme	current	491:497	arg1	study					499:503	the current study	487:503	the current study	487:503	The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide.
31475377	4	63	theme	average	574:580	arg1	1,322 kDa					615:623	1,322 kDa	615:623	1,322 kDa	615:623	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	4	63	theme	average	574:580	arg1	weight					592:597	The average molecular weight	570:597	The average molecular weight	570:597	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
31475377	9	64	theme	antioxidant	1436:1446	arg1	potential					1448:1456	antioxidant potential	1436:1456	antioxidant potential	1436:1456	This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG.
31475377	2	65	theme	water	330:334	arg1	method					347:352	hot water extraction method	326:352	hot water extraction method	326:352	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	7	66	theme	s	1235:1235	arg1	pectra					1237:1242	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	0	67	theme	structural	49:58	arg1	characterization					60:75	structural characterization	49:75	structural characterization	49:75	Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity.
31475377	6	68	theme	FSG	1130:1132	arg1	potency					1119:1125	the antioxidant potency	1103:1125	the antioxidant potency of FSG	1103:1132	The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG.
31475377	7	69	theme	resonance	1207:1215	arg1	pectra					1237:1242	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra	1186:1242	Fourier-transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1 H, 13 C) s pectra ratified the presence of functional groups typical for polysaccharide.
31475377	2	70	theme	hot	326:328	arg1	method					347:352	hot water extraction method	326:352	hot water extraction method	326:352	FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method.
31475377	4	71	theme	2.4	674:676	arg1	ratio					647:651	a polydispersity ratio	630:651	a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz	630:686	The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz.
30583371	5	0	theme	correlative	813:823	arg1	Levels					719:724	Levels	719:724	Levels of grape cell wall polysaccharide deconstruction during fermentation	719:793	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	5	0	theme	correlative	813:823	arg1	factor					825:830	a determining correlative factor	799:830	a determining correlative factor	799:830	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	7	1	theme	cell	1178:1181	arg1	deconstruction					1188:1201	cell wall deconstruction	1178:1201	cell wall deconstruction	1178:1201	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	6	2	theme	differential	1051:1062	arg1	extraction					1064:1073	the differential extraction	1047:1073	the differential extraction of specific polyphenols, especially polymeric polyphenols	1047:1131	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	5	3	theme	cell	735:738	arg1	deconstruction					760:773	grape cell wall polysaccharide deconstruction	729:773	grape cell wall polysaccharide deconstruction	729:773	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	7	4	theme	wall	1183:1186	arg1	deconstruction					1188:1201	cell wall deconstruction	1178:1201	cell wall deconstruction	1178:1201	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	2	5	theme	ripeness	363:370	arg1	levels					372:377	three different ripeness levels	347:377	three different ripeness levels	347:377	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	0	6	theme	phenolic	108:115	arg1	compounds					117:125	grape phenolic compounds	102:125	grape phenolic compounds	102:125	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	0	7	theme	grape	102:106	arg1	compounds					117:125	grape phenolic compounds	102:125	grape phenolic compounds	102:125	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	1	8	dep	Part	146:149	arg1	Extractability					155:168	Extractability	155:168	Part II: Extractability during fermentation into wines made from grapes of different ripeness levels.	146:246	Part II: Extractability during fermentation into wines made from grapes of different ripeness levels.
30583371	2	9	theme	wall	299:302	arg1	breakdown					304:312	cell wall breakdown	294:312	cell wall breakdown	294:312	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	7	10	theme	possible	1232:1239	arg1	retention					1241:1249	possible retention	1232:1249	possible retention	1232:1249	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	7	11	theme	polymer	1333:1339	arg1	length					1341:1346	polymer length	1333:1346	polymer length	1333:1346	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	6	12	theme	polymeric	1111:1119	arg1	polyphenols					1121:1131	polymeric polyphenols	1111:1131	polymeric polyphenols	1111:1131	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	6	12	theme	polymeric	1111:1119	arg1	polyphenols					1087:1097	specific polyphenols	1078:1097	specific polyphenols	1078:1097	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	2	13	theme	cell	294:297	arg1	breakdown					304:312	cell wall breakdown	294:312	cell wall breakdown	294:312	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	3	14	theme	Phenolic	460:467	arg1	differences					469:479	Phenolic differences	460:479	Phenolic differences between the ripeness treatments	460:511	Phenolic differences between the ripeness treatments were minimal after ¼ of the fermentation was completed.
30583371	0	15	theme	compounds	117:125	arg1	extractability					84:97	the extractability	80:97	the extractability of grape phenolic compounds into Shiraz wines	80:143	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	0	15	theme	compounds	117:125	arg1	composition					64:74	cell wall polysaccharide composition	39:74	cell wall polysaccharide composition	39:74	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	1	16	theme	levels	240:245	arg1	grapes					211:216	grapes	211:216	grapes of different ripeness levels	211:245	Part II: Extractability during fermentation into wines made from grapes of different ripeness levels.
30583371	6	17	theme	pomace	1003:1008	arg1	"					988:988	"opening-up"	977:988	"opening-up"	977:988	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	6	17	theme	pomace	1003:1008	arg1	deconstruction					959:972	this deconstruction	954:972	this deconstruction	954:972	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	7	18	theme	specific	1269:1276	arg1	proanthocyanidins					1284:1300	specific grape proanthocyanidins	1269:1300	specific grape proanthocyanidins	1269:1300	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	6	19	located	found	937:941	arg1	context					880:886	this context	875:886	this context	875:886	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	6	19	located	found	937:941	arg2	de-pectination					893:906	the de-pectination	889:906	the de-pectination observed during ripening	889:931	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	2	20	theme	Shiraz	320:325	arg1	grapes					327:332	Shiraz grapes	320:332	Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines	320:457	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	5	21	theme	grape	729:733	arg1	deconstruction					760:773	grape cell wall polysaccharide deconstruction	729:773	grape cell wall polysaccharide deconstruction	729:773	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	3	22	theme	ripeness	493:500	arg1	treatments					502:511	the ripeness treatments	489:511	the ripeness treatments	489:511	Phenolic differences between the ripeness treatments were minimal after ¼ of the fermentation was completed.
30583371	0	23	theme	Shiraz	132:137	arg1	wines					139:143	Shiraz wines	132:143	Shiraz wines	132:143	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	4	24	theme	25°Brix	660:666	arg1	grapes					668:673	25°Brix grapes	660:673	25°Brix grapes	660:673	However, colour and phenolic content were significantly higher in finished wines made from 25°Brix grapes compared to those from 21°Brix and 23°Brix.
30583371	0	25	theme	wall	44:47	arg1	composition					64:74	cell wall polysaccharide composition	39:74	cell wall polysaccharide composition	39:74	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	4	26	theme	phenolic	589:596	arg1	content					598:604	phenolic content	589:604	phenolic content	589:604	However, colour and phenolic content were significantly higher in finished wines made from 25°Brix grapes compared to those from 21°Brix and 23°Brix.
30583371	5	27	theme	wall	740:743	arg1	deconstruction					760:773	grape cell wall polysaccharide deconstruction	729:773	grape cell wall polysaccharide deconstruction	729:773	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	2	28	theme	alcoholic	425:433	arg1	fermentation					435:446	alcoholic fermentation	425:446	alcoholic fermentation into wines	425:457	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	0	29	theme	cell	39:42	arg1	composition					64:74	cell wall polysaccharide composition	39:74	cell wall polysaccharide composition	39:74	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	6	30	theme	grape	997:1001	arg1	pomace					1003:1008	the grape pomace	993:1008	the grape pomace	993:1008	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	5	31	theme	polysaccharide	745:758	arg1	deconstruction					760:773	grape cell wall polysaccharide deconstruction	729:773	grape cell wall polysaccharide deconstruction	729:773	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	1	32	theme	different	221:229	arg1	levels					240:245	different ripeness levels	221:245	different ripeness levels	221:245	Part II: Extractability during fermentation into wines made from grapes of different ripeness levels.
30583371	7	33	theme	deconstruction	1188:1201	arg1	degree					1168:1173	the degree	1164:1173	the degree of cell wall deconstruction	1164:1201	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	5	34	theme	deconstruction	760:773	arg1	Levels					719:724	Levels	719:724	Levels of grape cell wall polysaccharide deconstruction during fermentation	719:793	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	5	34	theme	deconstruction	760:773	arg1	factor					825:830	a determining correlative factor	799:830	a determining correlative factor	799:830	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	5	35	theme	phenolic	847:854	arg1	extractability					856:869	phenolic extractability	847:869	phenolic extractability	847:869	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	7	36	theme	proanthocyanidins	1284:1300	arg1	extraction					1255:1264	extraction	1255:1264	extraction	1255:1264	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	7	36	theme	proanthocyanidins	1284:1300	arg1	retention					1241:1249	possible retention	1232:1249	possible retention	1232:1249	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	2	37	theme	different	353:361	arg1	levels					372:377	three different ripeness levels	347:377	three different ripeness levels	347:377	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	0	38	theme	polysaccharide	49:62	arg1	composition					64:74	cell wall polysaccharide composition	39:74	cell wall polysaccharide composition	39:74	Investigating the relationship between cell wall polysaccharide composition and the extractability of grape phenolic compounds into Shiraz wines.
30583371	7	39	dep	retention	1241:1249	arg1	the					1228:1230	the	1228:1230	the	1228:1230	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	5	40	from	factor	825:830	arg1	relation					835:842	relation	835:842	relation to phenolic extractability	835:869	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	6	41	theme	specific	1078:1085	arg1	polyphenols					1121:1131	polymeric polyphenols	1111:1131	polymeric polyphenols	1111:1131	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	6	41	theme	specific	1078:1085	arg1	polyphenols					1087:1097	specific polyphenols	1078:1097	specific polyphenols	1078:1097	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	1	42	theme	ripeness	231:238	arg1	levels					240:245	different ripeness levels	221:245	different ripeness levels	221:245	Part II: Extractability during fermentation into wines made from grapes of different ripeness levels.
30583371	2	43	dep	colour	387:392	arg1	the					383:385	the	383:385	the	383:385	This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines.
30583371	4	44	theme	finished	635:642	arg1	wines					644:648	finished wines	635:648	finished wines made from 25°Brix grapes	635:673	However, colour and phenolic content were significantly higher in finished wines made from 25°Brix grapes compared to those from 21°Brix and 23°Brix.
30583371	7	45	theme	grape	1278:1282	arg1	proanthocyanidins					1284:1300	specific grape proanthocyanidins	1269:1300	specific grape proanthocyanidins	1269:1300	Additionally, the degree of cell wall deconstruction seemed to play a role in the possible retention and extraction of specific grape proanthocyanidins, depending on their nature and polymer length.
30583371	3	46	theme	fermentation	541:552	arg1	¼					532:532	¼	532:532	¼ of the fermentation	532:552	Phenolic differences between the ripeness treatments were minimal after ¼ of the fermentation was completed.
30583371	5	47	theme	determining	801:811	arg1	Levels					719:724	Levels	719:724	Levels of grape cell wall polysaccharide deconstruction during fermentation	719:793	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	5	47	theme	determining	801:811	arg1	factor					825:830	a determining correlative factor	799:830	a determining correlative factor	799:830	Levels of grape cell wall polysaccharide deconstruction during fermentation was a determining correlative factor in relation to phenolic extractability.
30583371	6	48	theme	opening-up	978:987	arg1	"					988:988	"opening-up"	977:988	"opening-up"	977:988	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
30583371	6	49	theme	polyphenols	1087:1097	arg1	extraction					1064:1073	the differential extraction	1047:1073	the differential extraction of specific polyphenols, especially polymeric polyphenols	1047:1131	In this context, the de-pectination observed during ripening was found to enhance this deconstruction or "opening-up" of the grape pomace during fermentation, thus increasing the differential extraction of specific polyphenols, especially polymeric polyphenols, into the wines.
31423779	4	0	theme	sulfate	862:868	arg1	isomers					899:905	sulfate positional isomers	880:905	sulfate positional isomers	880:905	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	0	theme	sulfate	862:868	arg1	patterns					870:877	sulfate patterns	862:877	sulfate patterns (sulfate positional isomers)	862:906	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	9	1	contain	has	1827:1829	arg1	LC-NETD-MS/MS					1813:1825	LC-NETD-MS/MS	1813:1825	LC-NETD-MS/MS	1813:1825	Thus, LC-NETD-MS/MS has great potential for further application to biological studies.
31423779	9	1	contain	has	1827:1829	arg2	potential					1837:1845	great potential	1831:1845	great potential for further application to biological studies	1831:1891	Thus, LC-NETD-MS/MS has great potential for further application to biological studies.
31423779	7	2	theme	unambiguous	1434:1444	arg1	assignment					1457:1466	unambiguous structural assignment	1434:1466	unambiguous structural assignment	1434:1466	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	6	3	theme	precursor	1146:1154	arg1	states					1163:1168	the precursor charge states	1142:1168	the precursor charge states	1142:1168	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	7	4	theme	3-O	1410:1412	arg1	sulfation					1414:1422	3-O sulfation	1410:1422	3-O sulfation	1410:1422	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	3	5	theme	LC-MS/MS	583:590	arg1	sequencing					592:601	online LC-MS/MS sequencing	576:601	online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD)	576:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	5	6	theme	fine	1048:1051	arg1	structure					1053:1061	their fine structure	1042:1061	their fine structure	1042:1061	The LC elution order of isomeric compounds was associated with their fine structure.
31423779	2	7	theme	profile	291:297	arg1	changes					299:305	profile changes	291:305	profile changes in HS composition	291:323	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	4	8	theme	acid	920:923	arg1	epimers					940:946	epimers	940:946	epimers	940:946	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	8	theme	acid	920:923	arg1	epimerization					925:937	uronic acid epimerization	913:937	uronic acid epimerization (epimers)	913:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	6	9	theme	glycosidic	1213:1222	arg1	fragments					1224:1232	abundant glycosidic fragments	1204:1232	abundant glycosidic fragments	1204:1232	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	10	theme	HS	1308:1309	arg1	oligosaccharides					1311:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	3	11	theme	oligosaccharides	609:624	arg1	sequencing					592:601	online LC-MS/MS sequencing	576:601	online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD)	576:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	4	12	theme	positional	888:897	arg1	isomers					899:905	sulfate positional isomers	880:905	sulfate positional isomers	880:905	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	12	theme	positional	888:897	arg1	patterns					870:877	sulfate patterns	862:877	sulfate patterns (sulfate positional isomers)	862:906	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	2	13	theme	mass	214:217	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	14	theme	modification	1786:1797	arg1	motifs					1799:1804	both predominant and rare modification motifs	1760:1804	both predominant and rare modification motifs	1760:1804	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	15	theme	protein	100:106	arg1	interactions					116:127	protein binding interactions	100:127	protein binding interactions	100:127	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	2	16	theme	composition	259:269	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	7	17	theme	diagnostic	1346:1355	arg1	ions					1368:1371	the diagnostic cross-ring ions	1342:1371	the diagnostic cross-ring ions	1342:1371	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	6	18	theme	sulfated	1279:1286	arg1	oligosaccharides					1311:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	3	19	theme	liquid	656:661	arg1	HILIC					679:683	HILIC	679:683	HILIC	679:683	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	19	theme	liquid	656:661	arg1	chromatography					663:676	hydrophilic interaction liquid chromatography	632:676	hydrophilic interaction liquid chromatography (HILIC)	632:684	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	1	20	theme	interactions	116:127	arg1	range					91:95	a wide range	84:95	a wide range of protein binding interactions key to normal and pathological physiology	84:169	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	2	21	theme	oligosaccharides	498:513	arg1	sequencing					477:486	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing	419:486	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	3	22	theme	hydrophilic	632:642	arg1	HILIC					679:683	HILIC	679:683	HILIC	679:683	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	22	theme	hydrophilic	632:642	arg1	chromatography					663:676	hydrophilic interaction liquid chromatography	632:676	hydrophilic interaction liquid chromatography (HILIC)	632:684	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	4	23	dep	length	797:802	arg1	hexamers					819:826	hexamers	819:826	hexamers	819:826	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	23	dep	length	797:802	arg1	tetramers					805:813	tetramers	805:813	tetramers	805:813	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	2	24	from	changes	299:305	arg1	composition					313:323	HS composition	310:323	HS composition	310:323	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	25	theme	binding	538:544	arg1	determinants					546:557	protein binding determinants	530:557	protein binding determinants	530:557	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	26	theme	disaccharide	246:257	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	4	27	theme	chain	791:795	arg1	length					797:802	chain length	791:802	chain length (tetramers and hexamers)	791:827	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	7	28	theme	structural	1446:1455	arg1	assignment					1457:1466	unambiguous structural assignment	1434:1466	unambiguous structural assignment	1434:1466	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	2	29	theme	spectrometry	453:464	arg1	sequencing					477:486	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing	419:486	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	30	theme	heterogeneous	1546:1558	arg1	mixtures					1563:1570	heterogeneous HS mixtures	1546:1570	heterogeneous HS mixtures	1546:1570	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	4	31	theme	HS	760:761	arg1	oligosaccharides					763:778	synthetic HS oligosaccharides	750:778	synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers)	750:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	2	32	theme	sulfate	376:382	arg1	group					384:388	the labile sulfate group	365:388	the labile sulfate group	365:388	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	32	theme	sulfate	376:382	arg1	modifications					347:359	modifications	347:359	modifications	347:359	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	0	33	theme	Sequencing	0:9	arg1	Sulfate					19:25	Sequencing Heparan Sulfate	0:25	Sequencing Heparan Sulfate	0:25	Sequencing Heparan Sulfate Using HILIC LC-NETD-MS/MS.
31423779	2	34	theme	tandem	441:446	arg1	LC-MS/MS					467:474	LC-MS/MS	467:474	LC-MS/MS	467:474	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	34	theme	tandem	441:446	arg1	spectrometry					453:464	liquid chromatography tandem mass spectrometry	419:464	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	35	theme	mixtures	1563:1570	arg1	sequencing					1532:1541	sequencing	1532:1541	sequencing of heterogeneous HS mixtures	1532:1570	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	9	36	theme	great	1831:1835	arg1	potential					1837:1845	great potential	1831:1845	great potential for further application to biological studies	1831:1891	Thus, LC-NETD-MS/MS has great potential for further application to biological studies.
31423779	2	37	theme	liquid	419:424	arg1	LC-MS/MS					467:474	LC-MS/MS	467:474	LC-MS/MS	467:474	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	37	theme	liquid	419:424	arg1	spectrometry					453:464	liquid chromatography tandem mass spectrometry	419:464	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	0	38	theme	HILIC	33:37	arg1	LC-NETD-MS/MS					39:51	HILIC LC-NETD-MS/MS	33:51	HILIC LC-NETD-MS/MS	33:51	Sequencing Heparan Sulfate Using HILIC LC-NETD-MS/MS.
31423779	4	39	theme	sulfate	840:846	arg1	3-7					856:858	3-7	856:858	3-7	856:858	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	39	theme	sulfate	840:846	arg1	groups					848:853	sulfate groups	840:853	sulfate groups (3-7)	840:859	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	5	40	theme	compounds	1012:1020	arg1	order					994:998	The LC elution order	979:998	The LC elution order of isomeric compounds	979:1020	The LC elution order of isomeric compounds was associated with their fine structure.
31423779	6	41	theme	device	1109:1114	arg1	application					1068:1078	The application	1064:1078	The application of an online cation exchange device (ion suppressor)	1064:1131	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	9	42	theme	biological	1874:1883	arg1	studies					1885:1891	biological studies	1874:1891	biological studies	1874:1891	Thus, LC-NETD-MS/MS has great potential for further application to biological studies.
31423779	3	43	theme	transfer	708:715	arg1	NETD					731:734	NETD	731:734	NETD	731:734	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	43	theme	transfer	708:715	arg1	dissociation					717:728	negative electron transfer dissociation	690:728	negative electron transfer dissociation (NETD)	690:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	6	44	theme	cation	1093:1098	arg1	device					1109:1114	an online cation exchange device	1083:1114	an online cation exchange device (ion suppressor)	1083:1131	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	44	theme	cation	1093:1098	arg1	suppressor					1121:1130	ion suppressor	1117:1130	ion suppressor	1117:1130	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	3	45	theme	negative	690:697	arg1	NETD					731:734	NETD	731:734	NETD	731:734	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	45	theme	negative	690:697	arg1	dissociation					717:728	negative electron transfer dissociation	690:728	negative electron transfer dissociation (NETD)	690:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	8	46	theme	mixtures	1682:1689	arg1	characterization					1651:1666	the characterization	1647:1666	the characterization of saccharide mixtures with a varying extent of sulfation	1647:1724	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	5	47	theme	elution	986:992	arg1	order					994:998	The LC elution order	979:998	The LC elution order of isomeric compounds	979:1020	The LC elution order of isomeric compounds was associated with their fine structure.
31423779	2	48	theme	modifications	347:359	arg1	group					384:388	the labile sulfate group	365:388	the labile sulfate group	365:388	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	48	theme	modifications	347:359	arg1	modifications					347:359	modifications	347:359	modifications	347:359	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	48	theme	modifications	347:359	arg1	heterogeneity					330:342	the heterogeneity	326:342	the heterogeneity of modifications	326:359	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	3	49	theme	online	576:581	arg1	sequencing					592:601	online LC-MS/MS sequencing	576:601	online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD)	576:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	6	50	theme	charge	1156:1161	arg1	states					1163:1168	the precursor charge states	1142:1168	the precursor charge states	1142:1168	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	8	51	theme	sulfation	1716:1724	arg1	extent					1706:1711	a varying extent	1696:1711	a varying extent of sulfation	1696:1724	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	52	theme	pathological	147:158	arg1	physiology					160:169	normal and pathological physiology	136:169	normal and pathological physiology	136:169	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	4	53	theme	groups	848:853	arg1	length					797:802	chain length	791:802	chain length (tetramers and hexamers)	791:827	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	53	theme	groups	848:853	arg1	isomers					899:905	sulfate positional isomers	880:905	sulfate positional isomers	880:905	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	53	theme	groups	848:853	arg1	number					830:835	number	830:835	number of sulfate groups (3-7)	830:859	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	53	theme	groups	848:853	arg1	epimerization					925:937	uronic acid epimerization	913:937	uronic acid epimerization (epimers)	913:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	53	theme	groups	848:853	arg1	epimers					940:946	epimers	940:946	epimers	940:946	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	53	theme	groups	848:853	arg1	patterns					870:877	sulfate patterns	862:877	sulfate patterns (sulfate positional isomers)	862:906	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	2	54	theme	HS	310:311	arg1	composition					313:323	HS composition	310:323	HS composition	310:323	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	6	55	theme	ion	1117:1119	arg1	device					1109:1114	an online cation exchange device	1083:1114	an online cation exchange device (ion suppressor)	1083:1131	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	55	theme	ion	1117:1119	arg1	suppressor					1121:1130	ion suppressor	1117:1130	ion suppressor	1117:1130	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	56	theme	oligosaccharides	1311:1326	arg1	characterization					1248:1263	the characterization	1244:1263	the characterization of both lowly sulfated and highly sulfated HS oligosaccharides	1244:1326	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	3	57	theme	HS	606:607	arg1	oligosaccharides					609:624	HS oligosaccharides	606:624	HS oligosaccharides	606:624	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	4	58	theme	uronic	913:918	arg1	epimers					940:946	epimers	940:946	epimers	940:946	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	58	theme	uronic	913:918	arg1	epimerization					925:937	uronic acid epimerization	913:937	uronic acid epimerization (epimers)	913:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	7	59	theme	6-O	1392:1394	arg1	sulfation					1396:1404	the 6-O sulfation	1388:1404	the 6-O sulfation	1388:1404	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	6	60	theme	sulfated	1299:1306	arg1	oligosaccharides					1311:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	both lowly sulfated and highly sulfated HS oligosaccharides	1268:1326	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	2	61	theme	spectrometry	219:230	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	62	theme	predominant	1765:1775	arg1	motifs					1799:1804	both predominant and rare modification motifs	1760:1804	both predominant and rare modification motifs	1760:1804	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	4	63	theme	sulfate	880:886	arg1	isomers					899:905	sulfate positional isomers	880:905	sulfate positional isomers	880:905	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	4	63	theme	sulfate	880:886	arg1	patterns					870:877	sulfate patterns	862:877	sulfate patterns (sulfate positional isomers)	862:906	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	8	64	theme	LC-NETD-MS/MS	1488:1500	arg1	tool					1523:1526	a powerful tool	1512:1526	a powerful tool for sequencing of heterogeneous HS mixtures	1512:1570	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	8	64	theme	LC-NETD-MS/MS	1488:1500	arg1	method					1502:1507	this LC-NETD-MS/MS method	1483:1507	this LC-NETD-MS/MS method	1483:1507	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	3	65	theme	interaction	644:654	arg1	HILIC					679:683	HILIC	679:683	HILIC	679:683	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	65	theme	interaction	644:654	arg1	chromatography					663:676	hydrophilic interaction liquid chromatography	632:676	hydrophilic interaction liquid chromatography (HILIC)	632:684	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	8	66	theme	rare	1781:1784	arg1	motifs					1799:1804	both predominant and rare modification motifs	1760:1804	both predominant and rare modification motifs	1760:1804	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	67	theme	binding	108:114	arg1	interactions					116:127	protein binding interactions	100:127	protein binding interactions	100:127	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	7	68	theme	cross-ring	1357:1366	arg1	ions					1368:1371	the diagnostic cross-ring ions	1342:1371	the diagnostic cross-ring ions	1342:1371	Furthermore, the diagnostic cross-ring ions differentiated the 6-O sulfation and 3-O sulfation, allowing unambiguous structural assignment.
31423779	2	69	theme	based	240:244	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	70	theme	motifs	1799:1804	arg1	determination					1743:1755	the determination	1739:1755	the determination of both predominant and rare modification motifs	1739:1804	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	71	theme	key	129:131	arg1	range					91:95	a wide range	84:95	a wide range of protein binding interactions key to normal and pathological physiology	84:169	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	2	72	theme	HS	495:496	arg1	oligosaccharides					498:513	the HS oligosaccharides	491:513	the HS oligosaccharides	491:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	73	theme	powerful	1514:1521	arg1	tool					1523:1526	a powerful tool	1512:1526	a powerful tool for sequencing of heterogeneous HS mixtures	1512:1570	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	8	73	theme	powerful	1514:1521	arg1	method					1502:1507	this LC-NETD-MS/MS method	1483:1507	this LC-NETD-MS/MS method	1483:1507	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	74	theme	normal	136:141	arg1	physiology					160:169	normal and pathological physiology	136:169	normal and pathological physiology	136:169	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	2	75	theme	protein	530:536	arg1	determinants					546:557	protein binding determinants	530:557	protein binding determinants	530:557	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	76	theme	HS	1560:1561	arg1	mixtures					1563:1570	heterogeneous HS mixtures	1546:1570	heterogeneous HS mixtures	1546:1570	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	0	77	theme	Heparan	11:17	arg1	Sulfate					19:25	Sequencing Heparan Sulfate	0:25	Sequencing Heparan Sulfate	0:25	Sequencing Heparan Sulfate Using HILIC LC-NETD-MS/MS.
31423779	2	78	theme	mass	448:451	arg1	LC-MS/MS					467:474	LC-MS/MS	467:474	LC-MS/MS	467:474	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	78	theme	mass	448:451	arg1	spectrometry					453:464	liquid chromatography tandem mass spectrometry	419:464	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	79	theme	liquid	179:184	arg1	chromatography					186:199	liquid chromatography	179:199	liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis	179:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	4	80	theme	synthetic	750:758	arg1	oligosaccharides					763:778	synthetic HS oligosaccharides	750:778	synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers)	750:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	2	81	theme	labile	369:374	arg1	group					384:388	the labile sulfate group	365:388	the labile sulfate group	365:388	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	81	theme	labile	369:374	arg1	modifications					347:359	modifications	347:359	modifications	347:359	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	1	82	theme	wide	86:89	arg1	range					91:95	a wide range	84:95	a wide range of protein binding interactions key to normal and pathological physiology	84:169	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	2	83	theme	LC-MS	233:237	arg1	analysis					271:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	mass spectrometry (LC-MS) based disaccharide composition analysis	214:278	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	84	theme	chromatography	426:439	arg1	LC-MS/MS					467:474	LC-MS/MS	467:474	LC-MS/MS	467:474	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	2	84	theme	chromatography	426:439	arg1	spectrometry					453:464	liquid chromatography tandem mass spectrometry	419:464	liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides	419:513	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	8	85	with	mixtures	1682:1689	arg1	extent					1706:1711	a varying extent	1696:1711	a varying extent of sulfation	1696:1724	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	4	86	theme	oligosaccharides	763:778	arg1	series					740:745	A series	738:745	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers)	738:947	A series of synthetic HS oligosaccharides varying in chain length (tetramers and hexamers), number of sulfate groups (3-7), sulfate patterns (sulfate positional isomers), and uronic acid epimerization (epimers) were separated and sequenced.
31423779	9	87	theme	further	1851:1857	arg1	application					1859:1869	further application	1851:1869	further application to biological studies	1851:1891	Thus, LC-NETD-MS/MS has great potential for further application to biological studies.
31423779	2	88	theme	major	398:402	arg1	challenges					404:413	major challenges	398:413	major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants	398:557	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	5	89	theme	isomeric	1003:1010	arg1	compounds					1012:1020	isomeric compounds	1003:1020	isomeric compounds	1003:1020	The LC elution order of isomeric compounds was associated with their fine structure.
31423779	2	90	gly	heterogeneity	330:342	arg1	modifications					347:359	modifications	347:359	modifications	347:359	Though liquid chromatography coupled with mass spectrometry (LC-MS) based disaccharide composition analysis is able to profile changes in HS composition, the heterogeneity of modifications and the labile sulfate group present major challenges for liquid chromatography tandem mass spectrometry (LC-MS/MS) sequencing of the HS oligosaccharides that represent protein binding determinants.
31423779	3	91	theme	electron	699:706	arg1	NETD					731:734	NETD	731:734	NETD	731:734	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	3	91	theme	electron	699:706	arg1	dissociation					717:728	negative electron transfer dissociation	690:728	negative electron transfer dissociation (NETD)	690:735	Here, we report online LC-MS/MS sequencing of HS oligosaccharides using hydrophilic interaction liquid chromatography (HILIC) and negative electron transfer dissociation (NETD).
31423779	8	92	theme	saccharide	1671:1680	arg1	mixtures					1682:1689	saccharide mixtures	1671:1689	saccharide mixtures with a varying extent of sulfation	1671:1724	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	1	93	theme	Heparan	54:60	arg1	HS					71:72	HS	71:72	HS	71:72	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	1	93	theme	Heparan	54:60	arg1	sulfate					62:68	Heparan sulfate	54:68	Heparan sulfate (HS)	54:73	Heparan sulfate (HS) mediates a wide range of protein binding interactions key to normal and pathological physiology.
31423779	6	94	theme	abundant	1204:1211	arg1	fragments					1224:1232	abundant glycosidic fragments	1204:1232	abundant glycosidic fragments	1204:1232	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	95	theme	exchange	1100:1107	arg1	device					1109:1114	an online cation exchange device	1083:1114	an online cation exchange device (ion suppressor)	1083:1131	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	95	theme	exchange	1100:1107	arg1	suppressor					1121:1130	ion suppressor	1117:1130	ion suppressor	1117:1130	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	8	96	theme	isomers	1622:1628	arg1	differentiation					1598:1612	the differentiation	1594:1612	the differentiation of both isomers and epimers	1594:1640	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	5	97	theme	LC	983:984	arg1	order					994:998	The LC elution order	979:998	The LC elution order of isomeric compounds	979:1020	The LC elution order of isomeric compounds was associated with their fine structure.
31423779	6	98	theme	online	1086:1091	arg1	device					1109:1114	an online cation exchange device	1083:1114	an online cation exchange device (ion suppressor)	1083:1131	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	6	98	theme	online	1086:1091	arg1	suppressor					1121:1130	ion suppressor	1117:1130	ion suppressor	1117:1130	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	8	99	theme	epimers	1634:1640	arg1	differentiation					1598:1612	the differentiation	1594:1612	the differentiation of both isomers and epimers	1594:1640	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
31423779	6	100	theme	subsequent	1179:1188	arg1	NETD					1190:1193	the subsequent NETD	1175:1193	the subsequent NETD	1175:1193	The application of an online cation exchange device (ion suppressor) enhanced the precursor charge states, and the subsequent NETD produced abundant glycosidic fragments, allowing the characterization of both lowly sulfated and highly sulfated HS oligosaccharides.
31423779	8	101	theme	varying	1698:1704	arg1	extent					1706:1711	a varying extent	1696:1711	a varying extent of sulfation	1696:1724	Collectively, this LC-NETD-MS/MS method is a powerful tool for sequencing of heterogeneous HS mixtures and is applicable for the differentiation of both isomers and epimers, for the characterization of saccharide mixtures with a varying extent of sulfation and even for the determination of both predominant and rare modification motifs.
30968121	0	0	theme	added	186:190	arg1	enzymes					202:208	added composite enzymes	186:208	added composite enzymes	186:208	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	11	1	theme	DC	1962:1963	arg1	values					1965:1970	Higher DC values	1955:1970	Higher DC values for S-NCP	1955:1980	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	4	2	theme	isocaloric	758:767	arg1	ME					784:785	isocaloric, 3,020 kcal/kg ME	758:785	isocaloric, 3,020 kcal/kg ME	758:785	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	8	3	from	samples	1342:1348	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	1	4	theme	feed	283:286	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	5	theme	P	1459:1459	arg1	variation					1448:1456	variation	1448:1456	variation (P < 0.05) in NCP levels	1448:1481	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	5	theme	P	1459:1459	arg1	<					1461:1461	P < 0.05	1459:1466	P < 0.05	1459:1466	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	2	6	dep	enzymes	564:570	arg1	multi-carbohydrases					583:601	multi-carbohydrases	583:601	multi-carbohydrases	583:601	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	2	6	dep	enzymes	564:570	arg1	proteases					608:616	proteases	608:616	proteases	608:616	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	2	6	dep	enzymes	564:570	arg1	phytases					573:580	phytases	573:580	phytases	573:580	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	1	7	theme	ingredient	288:297	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	6	8	theme	42-day-old	1027:1036	arg1	birds					1038:1042	42-day-old birds	1027:1042	42-day-old birds	1027:1042	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	10	9	from	day	1792:1794	arg1	values					1782:1787	The ileal DC values	1769:1787	The ileal DC values at day 42	1769:1797	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	10	9	from	day	1792:1794	arg1	different					1808:1816	different	1808:1816	different	1808:1816	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	6	10	theme	insoluble	1096:1104	arg1	I-NCP					1111:1115	I-NCP	1111:1115	I-NCP	1111:1115	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	6	10	theme	insoluble	1096:1104	arg1	NCP					1106:1108	insoluble NCP	1096:1108	insoluble NCP (I-NCP)	1096:1116	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	9	11	dep	treated	1558:1564	arg1	E					1572:1572	E	1572:1572	E	1572:1572	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	9	11	dep	treated	1558:1564	arg1	NC					1567:1568	NC	1567:1568	NC	1567:1568	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	12	12	from	use	2120:2122	arg1	diets					2166:2170	corn-soy-based diets	2151:2170	corn-soy-based diets	2151:2170	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	10	13	theme	NC	1915:1916	arg1	diet					1922:1925	NC + E diet	1915:1925	diet	1922:1925	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	8	14	from	analysis	1268:1275	arg1	Results					1240:1246	Results	1240:1246	Results from the ingredient analysis	1240:1275	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	1	15	theme	initial	214:220	arg1	study					222:226	An initial study	211:226	An initial study	211:226	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	4	16	theme	3,026 kcal/kg	807:819	arg1	ME					821:822	3,026 kcal/kg ME	807:822	3,026 kcal/kg ME	807:822	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	2	17	from	day	462:464	arg1	broilers					450:457	broilers	450:457	broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases)	450:617	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	7	18	theme	feeding	1223:1229	arg1	periods					1231:1237	both the feeding periods	1214:1237	both the feeding periods	1214:1237	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	3	19	theme	positive	678:685	arg1	PC					696:697	PC	696:697	PC	696:697	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	3	19	theme	positive	678:685	arg1	control					687:693	positive control	678:693	positive control (PC)	678:698	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	1	20	from	levels	273:278	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	4	21	from	phase	797:801	arg1	ME					784:785	isocaloric, 3,020 kcal/kg ME	758:785	isocaloric, 3,020 kcal/kg ME	758:785	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	12	22	theme	corn-soy-based	2151:2164	arg1	diets					2166:2170	corn-soy-based diets	2151:2170	corn-soy-based diets	2151:2170	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	2	23	theme	based	525:529	arg1	diets					531:535	fed corn-soy based diets	512:535	fed corn-soy based diets	512:535	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	10	24	theme	E	1920:1920	arg1	diet					1922:1925	NC + E diet	1915:1925	diet	1922:1925	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	10	25	theme	DC	1865:1866	arg1	values					1868:1873	total tract DC values	1853:1873	total tract DC values	1853:1873	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	2	26	theme	fed	512:514	arg1	diets					531:535	fed corn-soy based diets	512:535	fed corn-soy based diets	512:535	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	10	27	theme	DC	1779:1780	arg1	values					1782:1787	The ileal DC values	1769:1787	The ileal DC values at day 42	1769:1797	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	10	27	theme	DC	1779:1780	arg1	different					1808:1816	different	1808:1816	different	1808:1816	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	6	28	dep	NCP	1080:1082	arg1	fractions					1118:1126	fractions	1118:1126	fractions	1118:1126	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	12	29	theme	total	2194:2198	arg1	digestion					2204:2212	total NCP digestion	2194:2212	total NCP digestion	2194:2212	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	12	30	from	improvement	2179:2189	arg1	digestion					2204:2212	total NCP digestion	2194:2212	total NCP digestion	2194:2212	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	2	31	theme	grower	470:475	arg1	day					462:464	day 21	462:467	day 21 (grower phase)	462:482	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	2	31	theme	grower	470:475	arg1	phase					477:481	grower phase	470:481	grower phase	470:481	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	1	32	dep	levels	273:278	arg1	grains					369:374	dried grains	363:374	dried grains with solubles (DDGS)	363:395	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	33	dep	17	1416:1417	arg1	to					1413:1414	to	1413:1414	to	1413:1414	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	34	from	samples	1316:1322	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	35	dep	10	1619:1620	arg1	to					1616:1617	to	1616:1617	to	1616:1617	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	36	from	content	30:36	arg1	broilers					150:157	42-day-old broilers	139:157	42-day-old broilers	139:157	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	0	36	from	content	30:36	arg1	ingredients					46:56	feed ingredients	41:56	feed ingredients	41:56	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	0	36	from	content	30:36	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	0	36	from	content	30:36	arg1	21-					131:133	21-	131:133	21-	131:133	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	9	37	theme	tract	1665:1669	arg1	collection					1671:1680	ileal and total tract collection	1649:1680	ileal and total tract collection	1649:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	38	theme	tract	78:82	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	2	39	from	day	488:490	arg1	broilers					450:457	broilers	450:457	broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases)	450:617	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	8	40	from	%	1418:1418	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	40	from	%	1418:1418	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	40	from	%	1418:1418	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	40	from	%	1418:1418	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	0	41	theme	polysaccharide	99:112	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	9	42	from	day	1602:1604	arg1	values					1592:1597	DC values	1589:1597	DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection	1589:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	5	43	theme	Positive	843:850	arg1	diet					860:863	Positive control diet	843:863	Positive control diet	843:863	Positive control diet was formulated to meet the Cobb standard nutrient specifications.
30968121	11	44	theme	∼0.8-total	2056:2065	arg1	tract					2067:2071	∼0.8-total tract	2056:2071	∼0.8-total tract	2056:2071	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	12	45	theme	supplemental	2127:2138	arg1	enzymes					2140:2146	supplemental enzymes	2127:2146	supplemental enzymes	2127:2146	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	4	46	from	ME	821:822	arg1	ME					784:785	isocaloric, 3,020 kcal/kg ME	758:785	isocaloric, 3,020 kcal/kg ME	758:785	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	1	47	theme	dried	363:367	arg1	grains					369:374	dried grains	363:374	dried grains with solubles (DDGS)	363:395	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	1	48	from	soybean	339:345	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	49	dep	10	1304:1305	arg1	to					1301:1302	to	1301:1302	to	1301:1302	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	50	theme	I-NCP	1701:1705	arg1	fraction					1707:1714	I-NCP fraction	1701:1714	I-NCP fraction (P < 0.05)	1701:1725	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	9	50	theme	I-NCP	1701:1705	arg1	<					1719:1719	P < 0.05	1717:1724	P < 0.05	1717:1724	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	51	theme	Non-cellulosic	0:13	arg1	content					30:36	Non-cellulosic polysaccharide content	0:36	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers	0:157	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	3	52	dep	control	629:635	arg1	diets					700:704	diets	700:704	diets	700:704	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	6	53	theme	pooled	938:943	arg1	digesta					945:951	pooled digesta	938:951	pooled digesta	938:951	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	2	54	theme	digestibility	413:425	arg1	assay					427:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	2	55	theme	separate	400:407	arg1	assay					427:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	1	56	from	distillers	352:361	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	9	57	theme	treated	1558:1564	arg1	diet					1575:1578	enzyme treated (NC + E) diet	1551:1578	enzyme treated (NC + E) diet	1551:1578	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	58	theme	feed	41:44	arg1	ingredients					46:56	feed ingredients	41:56	feed ingredients	41:56	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	5	59	theme	Cobb	892:895	arg1	specifications					915:928	the Cobb standard nutrient specifications	888:928	the Cobb standard nutrient specifications	888:928	Positive control diet was formulated to meet the Cobb standard nutrient specifications.
30968121	4	60	theme	Negative	719:726	arg1	control					728:734	Negative control	719:734	Negative control	719:734	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	2	61	dep	day	462:464	arg1	diets					531:535	fed corn-soy based diets	512:535	fed corn-soy based diets	512:535	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	5	62	theme	nutrient	906:913	arg1	specifications					915:928	the Cobb standard nutrient specifications	888:928	the Cobb standard nutrient specifications	888:928	Positive control diet was formulated to meet the Cobb standard nutrient specifications.
30968121	7	63	theme	Digestibility	1129:1141	arg1	DC					1156:1157	DC	1156:1157	DC	1156:1157	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	7	63	theme	Digestibility	1129:1141	arg1	values					1160:1165	Digestibility coefficient (DC) values	1129:1165	Digestibility coefficient (DC) values	1129:1165	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	3	64	theme	Negative	620:627	arg1	NC					638:639	NC	638:639	NC	638:639	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	3	64	theme	Negative	620:627	arg1	control					629:635	Negative control	620:635	Negative control (NC)	620:640	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	1	65	theme	non-cellulosic	237:250	arg1	NCP					268:270	NCP	268:270	NCP	268:270	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	1	65	theme	non-cellulosic	237:250	arg1	polysaccharide					252:265	non-cellulosic polysaccharide	237:265	non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn	237:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	6	66	theme	treatment	983:991	arg1	diets					993:997	all treatment diets	979:997	all treatment diets	979:997	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	8	67	theme	pectin	1373:1378	arg1	level					1380:1384	pectin level	1373:1384	pectin level	1373:1384	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	68	theme	NCP	1284:1286	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	69	from	%	1333:1333	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	69	from	%	1333:1333	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	69	from	%	1333:1333	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	69	from	%	1333:1333	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	70	theme	DC	1589:1590	arg1	values					1592:1597	DC values	1589:1597	DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection	1589:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	71	theme	composite	192:200	arg1	enzymes					202:208	added composite enzymes	186:208	added composite enzymes	186:208	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	4	72	from	phase	836:840	arg1	ME					784:785	isocaloric, 3,020 kcal/kg ME	758:785	isocaloric, 3,020 kcal/kg ME	758:785	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	4	73	theme	E	745:745	arg1	diets					747:751	NC + E diets	740:751	NC + E diets	740:751	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	6	74	theme	excreta	958:964	arg1	samples					966:972	excreta samples	958:972	excreta samples from all treatment diets	958:997	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	8	75	theme	whole	1390:1394	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	76	theme	PC	1759:1760	arg1	diets					1762:1766	PC diets	1759:1766	PC diets	1759:1766	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	1	77	from	meal	321:324	arg1	samples-corn					299:310	feed ingredient samples-corn	283:310	feed ingredient samples-corn	283:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	78	from	variation	1448:1456	arg1	levels					1476:1481	NCP levels	1472:1481	NCP levels	1472:1481	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	10	79	theme	tract	1859:1863	arg1	values					1868:1873	total tract DC values	1853:1873	total tract DC values	1853:1873	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	6	80	theme	NCP	1048:1050	arg1	content					1052:1058	NCP content	1048:1058	NCP content	1048:1058	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	3	81	theme	composite	648:656	arg1	enzymes					658:664	composite enzymes	648:664	composite enzymes	648:664	Negative control (NC), NC + composite enzymes (NC+E), and positive control (PC) diets were tested.
30968121	8	82	from	levels	1288:1293	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	82	from	levels	1288:1293	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	82	from	levels	1288:1293	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	82	from	levels	1288:1293	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	83	from	%	1360:1360	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	83	from	%	1360:1360	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	83	from	%	1360:1360	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	83	from	%	1360:1360	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	84	dep	9	1637:1637	arg1	to					1634:1635	to	1634:1635	to	1634:1635	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	2	85	theme	composite	554:562	arg1	enzymes					564:570	composite enzymes	554:570	composite enzymes (phytases, multi-carbohydrases, and proteases)	554:617	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	10	86	theme	ileal	1773:1777	arg1	values					1782:1787	The ileal DC values	1769:1787	The ileal DC values at day 42	1769:1797	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	10	86	theme	ileal	1773:1777	arg1	different					1808:1816	different	1808:1816	different	1808:1816	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	0	87	theme	42-day-old	139:148	arg1	broilers					150:157	42-day-old broilers	139:157	42-day-old broilers	139:157	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	10	88	dep	11	1889:1890	arg1	to					1886:1887	to	1886:1887	to	1886:1887	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	4	89	theme	grower	790:795	arg1	phase					797:801	grower phase	790:801	grower phase	790:801	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	10	90	theme	PC	1945:1946	arg1	diets					1948:1952	PC diets	1945:1952	PC diets	1945:1952	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	8	91	dep	14	1358:1359	arg1	to					1355:1356	to	1355:1356	to	1355:1356	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	92	theme	NCP	1472:1474	arg1	levels					1476:1481	NCP levels	1472:1481	NCP levels	1472:1481	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	2	93	theme	corn-soy	516:523	arg1	diets					531:535	fed corn-soy based diets	512:535	fed corn-soy based diets	512:535	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	8	94	theme	%	1306:1306	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	9	95	from	units	1622:1626	arg1	values					1592:1597	DC values	1589:1597	DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection	1589:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	12	96	theme	NCP	2200:2202	arg1	digestion					2204:2212	total NCP digestion	2194:2212	total NCP digestion	2194:2212	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	8	97	from	soybean	1396:1402	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	1	98	with	grains	369:374	arg1	DDGS					391:394	DDGS	391:394	DDGS	391:394	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	1	98	with	grains	369:374	arg1	solubles					381:388	solubles	381:388	solubles (DDGS)	381:395	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	99	theme	corn	1311:1314	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	6	100	theme	soluble	1072:1078	arg1	S-NCP					1085:1089	S-NCP	1085:1089	S-NCP	1085:1089	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	6	100	theme	soluble	1072:1078	arg1	NCP					1080:1082	soluble NCP	1072:1082	soluble NCP (S-NCP)	1072:1090	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	9	101	theme	ileal	1649:1653	arg1	collection					1671:1680	ileal and total tract collection	1649:1680	ileal and total tract collection	1649:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	102	theme	total	72:76	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	8	103	theme	ingredient	1498:1507	arg1	samples					1509:1515	ingredient samples	1498:1515	ingredient samples	1498:1515	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	11	104	theme	feeding	2024:2030	arg1	periods					2032:2038	both feeding periods	2019:2038	both feeding periods	2019:2038	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	7	105	theme	dietary	1191:1197	arg1	treatments					1199:1208	all dietary treatments	1187:1208	all dietary treatments for both the feeding periods	1187:1237	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	9	106	theme	total	1659:1663	arg1	collection					1671:1680	ileal and total tract collection	1649:1680	ileal and total tract collection	1649:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	107	theme	non-cellulosic	84:97	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	1	108	theme	whole	333:337	arg1	soybean					339:345	whole soybean	333:345	whole soybean	333:345	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	2	109	theme	finisher	496:503	arg1	phase					505:509	finisher phase	496:509	finisher phase	496:509	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	2	109	theme	finisher	496:503	arg1	day					488:490	day 42	488:493	day 42 (finisher phase)	488:510	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	12	110	theme	enzymes	2140:2146	arg1	use					2120:2122	the use	2116:2122	the use of supplemental enzymes in corn-soy-based diets	2116:2170	Overall, the use of supplemental enzymes in corn-soy-based diets showed improvement in total NCP digestion.
30968121	8	111	theme	%	1333:1333	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	10	112	theme	treatment	1826:1834	arg1	diets					1836:1840	treatment diets	1826:1840	treatment diets (∼0.6)	1826:1847	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	10	112	theme	treatment	1826:1834	arg1	∼0.6					1843:1846	∼0.6	1843:1846	∼0.6	1843:1846	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	8	113	from	%	1306:1306	arg1	soybean					1396:1402	whole soybean	1390:1402	whole soybean	1390:1402	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	113	from	%	1306:1306	arg1	samples					1316:1322	corn samples	1311:1322	corn samples	1311:1322	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	113	from	%	1306:1306	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	113	from	%	1306:1306	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	5	114	theme	control	852:858	arg1	diet					860:863	Positive control diet	843:863	Positive control diet	843:863	Positive control diet was formulated to meet the Cobb standard nutrient specifications.
30968121	4	115	theme	finisher	827:834	arg1	phase					836:840	finisher phase	827:840	finisher phase	827:840	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	8	116	theme	SBM	1338:1340	arg1	samples					1342:1348	SBM samples	1338:1348	SBM samples	1338:1348	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	10	117	theme	total	1853:1857	arg1	values					1868:1873	total tract DC values	1853:1873	total tract DC values	1853:1873	The ileal DC values at day 42 were not different between treatment diets (∼0.6) but total tract DC values improved 9 to 11 units for broilers fed NC + E diet compared to NC or PC diets.
30968121	0	118	theme	polysaccharide	15:28	arg1	content					30:36	Non-cellulosic polysaccharide content	0:36	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers	0:157	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	8	119	from	samples	1428:1434	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	11	120	dep	observed	1987:1994	arg1	compared					2074:2081	compared	2074:2081	compared to DC values for I-NCP	2074:2104	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	11	120	dep	observed	1987:1994	arg1	∼0.7-ileal					2041:2050	∼0.7-ileal	2041:2050	∼0.7-ileal	2041:2050	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	11	120	dep	observed	1987:1994	arg1	tract					2067:2071	∼0.8-total tract	2056:2071	∼0.8-total tract	2056:2071	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	9	121	from	units	1639:1643	arg1	values					1592:1597	DC values	1589:1597	DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection	1589:1680	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	2	122	theme	NCP	409:411	arg1	assay					427:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay	398:431	A separate NCP digestibility assay was performed in broilers at day 21 (grower phase) and day 42 (finisher phase) fed corn-soy based diets with and without composite enzymes (phytases, multi-carbohydrases, and proteases).
30968121	9	123	theme	P	1717:1717	arg1	fraction					1707:1714	I-NCP fraction	1701:1714	I-NCP fraction (P < 0.05)	1701:1725	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	9	123	theme	P	1717:1717	arg1	<					1719:1719	P < 0.05	1717:1724	P < 0.05	1717:1724	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	4	124	dep	isocaloric	758:767	arg1	3,020 kcal/kg					770:782	3,020 kcal/kg	770:782	3,020 kcal/kg	770:782	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	1	125	theme	soybean	313:319	arg1	SBM					327:329	SBM	327:329	SBM	327:329	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	1	125	theme	soybean	313:319	arg1	meal					321:324	soybean meal	313:324	soybean meal (SBM)	313:330	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	7	126	theme	coefficient	1143:1153	arg1	DC					1156:1157	DC	1156:1157	DC	1156:1157	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	7	126	theme	coefficient	1143:1153	arg1	values					1160:1165	Digestibility coefficient (DC) values	1129:1165	Digestibility coefficient (DC) values	1129:1165	Digestibility coefficient (DC) values were determined for all dietary treatments for both the feeding periods.
30968121	8	127	theme	%	1360:1360	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	6	128	from	diets	993:997	arg1	Diets					931:935	Diets	931:935	Diets	931:935	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	6	128	from	diets	993:997	arg1	digesta					945:951	pooled digesta	938:951	pooled digesta	938:951	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	6	128	from	diets	993:997	arg1	samples					966:972	excreta samples	958:972	excreta samples from all treatment diets	958:997	Diets, pooled digesta, and excreta samples from all treatment diets were collected from 21- and 42-day-old birds and NCP content analyzed as soluble NCP (S-NCP) and insoluble NCP (I-NCP) fractions.
30968121	9	129	theme	Digestibility	1518:1530	arg1	assays					1532:1537	Digestibility assays	1518:1537	Digestibility assays	1518:1537	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	0	130	theme	ileal	62:66	arg1	digestibility					114:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	ileal and total tract non-cellulosic polysaccharide digestibility	62:126	Non-cellulosic polysaccharide content in feed ingredients and ileal and total tract non-cellulosic polysaccharide digestibility in 21- and 42-day-old broilers fed diets with and without added composite enzymes.
30968121	5	131	theme	standard	897:904	arg1	specifications					915:928	the Cobb standard nutrient specifications	888:928	the Cobb standard nutrient specifications	888:928	Positive control diet was formulated to meet the Cobb standard nutrient specifications.
30968121	4	132	theme	NC	740:741	arg1	diets					747:751	NC + E diets	740:751	NC + E diets	740:751	Negative control and NC + E diets were isocaloric, 3,020 kcal/kg ME at grower phase and 3,026 kcal/kg ME at finisher phase.
30968121	8	133	dep	%	1360:1360	arg1	including					1363:1371	including	1363:1371	including pectin level	1363:1384	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	11	134	theme	Higher	1955:1960	arg1	values					1965:1970	Higher DC values	1955:1970	Higher DC values for S-NCP	1955:1980	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	11	135	theme	DC	2086:2087	arg1	values					2089:2094	DC values	2086:2094	DC values for I-NCP	2086:2104	Higher DC values for S-NCP were observed for all treatments for both feeding periods (∼0.7-ileal and ∼0.8-total tract) compared to DC values for I-NCP.
30968121	9	136	theme	DC	1739:1740	arg1	values					1742:1747	DC values	1739:1747	DC values for NC or PC diets	1739:1766	Digestibility assays showed that enzyme treated (NC + E) diet improved DC values at day 21 from 6 to 10 units and 6 to 9 units for ileal and total tract collection, respectively, for I-NCP fraction (P < 0.05) compared to DC values for NC or PC diets.
30968121	8	137	theme	ingredient	1257:1266	arg1	analysis					1268:1275	the ingredient analysis	1253:1275	the ingredient analysis	1253:1275	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	138	theme	%	1418:1418	arg1	levels					1288:1293	NCP levels	1284:1293	NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples	1284:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	1	139	theme	polysaccharide	252:265	arg1	levels					273:278	non-cellulosic polysaccharide (NCP) levels	237:278	non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn	237:310	An initial study profiled non-cellulosic polysaccharide (NCP) levels in feed ingredient samples-corn, soybean meal (SBM), whole soybean, and distillers dried grains with solubles (DDGS).
30968121	8	140	theme	DDGS	1423:1426	arg1	samples					1428:1434	DDGS samples	1423:1434	DDGS samples	1423:1434	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30968121	8	141	dep	11	1331:1332	arg1	to					1328:1329	to	1328:1329	to	1328:1329	Results from the ingredient analysis showed NCP levels of ∼7 to 10% in corn samples, ∼8 to 11% in SBM samples, ∼11 to 14% (including pectin level) in whole soybean, and ∼12 to 17% in DDGS samples, suggesting variation (P < 0.05) in NCP levels existed within ingredient samples.
30058793	9	0	theme	tumor	1764:1768	arg1	relapse					1770:1776	tumor relapse	1764:1776	tumor relapse	1764:1776	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	10	1	theme	other	2053:2057	arg1	composites					2072:2081	other inorganic 2D composites	2053:2081	other inorganic 2D composites for applications in biomedicine	2053:2113	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	1	2	theme	photothermal	308:319	arg1	PTT					330:332	PTT	330:332	PTT	330:332	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	1	2	theme	photothermal	308:319	arg1	therapy					321:327	anticancer photothermal therapy	297:327	anticancer photothermal therapy (PTT)	297:333	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	5	3	theme	response	913:920	arg1	characteristics					947:961	rapid response near-infrared-stimulated characteristics	907:961	rapid response near-infrared-stimulated characteristics	907:961	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	9	4	theme	nanoplatform	1814:1825	arg1	potential					1797:1805	the potential	1793:1805	the potential of the nanoplatform for application in cancer therapy	1793:1859	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	8	5	theme	DOX	1447:1449	arg1	release					1436:1442	controlled, sustained release	1414:1442	controlled, sustained release of DOX	1414:1449	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	8	5	theme	DOX	1447:1449	arg1	performance					1398:1408	their excellent photothermal performance	1369:1408	their excellent photothermal performance	1369:1408	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	10	6	theme	2D	2069:2070	arg1	composites					2072:2081	other inorganic 2D composites	2053:2081	other inorganic 2D composites for applications in biomedicine	2053:2113	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	5	7	theme	composite	879:887	arg1	hydrogels					889:897	the cellulose/MXene composite hydrogels	859:897	the cellulose/MXene composite hydrogels	859:897	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	8	8	theme	tumor	1533:1537	arg1	treatment					1539:1547	tumor treatment	1533:1547	tumor treatment	1533:1547	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	0	9	theme	Bimodal	153:159	arg1	Activity					198:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	3	10	theme	MXene-based	649:659	arg1	nanoplatform					661:672	a smart MXene-based nanoplatform	641:672	a smart MXene-based nanoplatform for the treatment of cancer	641:700	Fabricating a smart MXene-based nanoplatform for the treatment of cancer therefore remains a challenge.
30058793	9	11	theme	cancer	1846:1851	arg1	therapy					1853:1859	cancer therapy	1846:1859	cancer therapy	1846:1859	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	0	12	theme	Anticancer	187:196	arg1	Activity					198:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	5	13	theme	continuous	983:992	arg1	process					1002:1008	a continuous dynamic process	981:1008	a continuous dynamic process in water	981:1017	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	2	14	theme	cancer	613:618	arg1	therapy					620:626	cancer therapy	613:626	cancer therapy	613:626	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	8	15	theme	excellent	1375:1383	arg1	performance					1398:1408	their excellent photothermal performance	1369:1408	their excellent photothermal performance	1369:1408	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	2	16	theme	targeting	555:563	arg1	capability					565:574	targeting capability	555:574	targeting capability	555:574	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	1	17	theme	outstanding	351:361	arg1	performance					376:386	their outstanding photothermal performance	345:386	their outstanding photothermal performance	345:386	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	10	18	theme	nanocomposites	1936:1949	arg1	fabrication					1903:1913	the fabrication	1899:1913	the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer	1899:2004	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	9	19	theme	PTT	1619:1621	arg1	efficient					1704:1712	efficient	1704:1712	efficient	1704:1712	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	9	19	theme	PTT	1619:1621	arg1	combination					1604:1614	the combination	1600:1614	the combination of PTT	1600:1621	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	9	19	theme	PTT	1619:1621	arg1	chemotherapy					1646:1657	prolonged adjuvant chemotherapy	1627:1657	prolonged adjuvant chemotherapy delivered using this nanoplatform	1627:1691	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	6	20	theme	drug	1065:1068	arg1	doxorubicin					1070:1080	the anticancer drug doxorubicin	1050:1080	the anticancer drug doxorubicin hydrochloride (DOX)	1050:1100	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	6	20	theme	drug	1065:1068	arg1	DOX					1097:1099	DOX	1097:1099	DOX	1097:1099	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	4	21	theme	composite	747:755	arg1	hydrogels					757:765	composite hydrogels	747:765	composite hydrogels	747:765	In this work, composite hydrogels based on cellulose and Ti3C2 MXene, were synthesized for the first time.
30058793	0	22	theme	Smart	69:73	arg1	Nanoplatforms					101:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	1	23	theme	Two-dimensional	208:222	arg1	MXenes					229:234	Two-dimensional (2D) MXenes	208:234	Two-dimensional (2D) MXenes	208:234	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	2	24	theme	controlled	581:590	arg1	release					592:598	controlled release	581:598	controlled release of drug	581:606	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	0	25	theme	Network	93:99	arg1	Nanoplatforms					101:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	2	26	from	biocompatibility	495:510	arg1	release					592:598	controlled release	581:598	controlled release of drug	581:606	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	26	from	biocompatibility	495:510	arg1	conditions					543:552	physiological conditions	529:552	physiological conditions	529:552	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	26	from	biocompatibility	495:510	arg1	capability					565:574	targeting capability	555:574	targeting capability	555:574	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	27	theme	2D	426:427	arg1	nanomaterials					429:441	the other inorganic 2D nanomaterials	406:441	the other inorganic 2D nanomaterials	406:441	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	10	28	from	applications	2087:2098	arg1	biomedicine					2103:2113	biomedicine	2103:2113	biomedicine	2103:2113	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	2	29	theme	appropriate	483:493	arg1	biocompatibility					495:510	the appropriate biocompatibility	479:510	the appropriate biocompatibility	479:510	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	30	from	stability	516:524	arg1	release					592:598	controlled release	581:598	controlled release of drug	581:606	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	30	from	stability	516:524	arg1	conditions					543:552	physiological conditions	529:552	physiological conditions	529:552	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	30	from	stability	516:524	arg1	capability					565:574	targeting capability	555:574	targeting capability	555:574	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	31	theme	other	410:414	arg1	nanomaterials					429:441	the other inorganic 2D nanomaterials	406:441	the other inorganic 2D nanomaterials	406:441	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	0	32	theme	Two-Dimensional	0:14	arg1	Hydrogels					51:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels	0:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.	0:206	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	6	33	theme	cellulose/MXene	1107:1121	arg1	hydrogels					1123:1131	the cellulose/MXene hydrogels	1103:1131	the cellulose/MXene hydrogels	1103:1131	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	6	33	theme	cellulose/MXene	1107:1121	arg1	capable					1137:1143	capable	1137:1143	capable	1137:1143	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	8	34	theme	cellulose/MXene	1456:1470	arg1	hydrogels					1472:1480	the cellulose/MXene hydrogels	1452:1480	the cellulose/MXene hydrogels	1452:1480	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	8	34	theme	cellulose/MXene	1456:1470	arg1	nanoplatform					1516:1527	a multifunctional nanoplatform	1498:1527	a multifunctional nanoplatform for tumor treatment	1498:1547	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	0	35	dep	Hydrogels	51:59	arg1	Exhibiting					115:124	Exhibiting	115:124	Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity	115:205	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	2	36	theme	MXene-based	448:458	arg1	nanoplatforms					460:472	the MXene-based nanoplatforms	444:472	the MXene-based nanoplatforms	444:472	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	10	37	theme	application	1979:1989	arg1	fabrication					1903:1913	the fabrication	1899:1913	the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer	1899:2004	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	3	38	theme	cancer	695:700	arg1	treatment					682:690	the treatment	678:690	the treatment of cancer	678:700	Fabricating a smart MXene-based nanoplatform for the treatment of cancer therefore remains a challenge.
30058793	8	39	used	utilized	1486:1493	arg2	hydrogels					1472:1480	the cellulose/MXene hydrogels	1452:1480	the cellulose/MXene hydrogels	1452:1480	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	8	39	used	utilized	1486:1493	arg2	nanoplatform					1516:1527	a multifunctional nanoplatform	1498:1527	a multifunctional nanoplatform for tumor treatment	1498:1547	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	0	40	theme	Cellulose	41:49	arg1	Hydrogels					51:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels	0:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.	0:206	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	9	41	theme	tumor	1726:1730	arg1	destruction					1732:1742	instant tumor destruction	1718:1742	instant tumor destruction	1718:1742	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	8	42	theme	multifunctional	1500:1514	arg1	hydrogels					1472:1480	the cellulose/MXene hydrogels	1452:1480	the cellulose/MXene hydrogels	1452:1480	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	8	42	theme	multifunctional	1500:1514	arg1	nanoplatform					1516:1527	a multifunctional nanoplatform	1498:1527	a multifunctional nanoplatform for tumor treatment	1498:1547	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	7	43	theme	nm	1343:1344	arg1	light					1346:1350	an 808 nm light	1336:1350	an 808 nm light	1336:1350	This behavior is attributed to the expansion of the pores within the three-dimensional cellulose-based networks, triggered by illumination with an 808 nm light.
30058793	7	44	theme	pores	1244:1248	arg1	expansion					1227:1235	the expansion	1223:1235	the expansion	1223:1235	This behavior is attributed to the expansion of the pores within the three-dimensional cellulose-based networks, triggered by illumination with an 808 nm light.
30058793	7	45	theme	cellulose-based	1279:1293	arg1	networks					1295:1302	the three-dimensional cellulose-based networks	1257:1302	the three-dimensional cellulose-based networks	1257:1302	This behavior is attributed to the expansion of the pores within the three-dimensional cellulose-based networks, triggered by illumination with an 808 nm light.
30058793	1	46	theme	anticancer	297:306	arg1	PTT					330:332	PTT	330:332	PTT	330:332	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	1	46	theme	anticancer	297:306	arg1	therapy					321:327	anticancer photothermal therapy	297:327	anticancer photothermal therapy (PTT)	297:333	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	8	47	theme	controlled	1414:1423	arg1	release					1436:1442	controlled, sustained release	1414:1442	controlled, sustained release of DOX	1414:1449	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	5	48	theme	rapid	907:911	arg1	characteristics					947:961	rapid response near-infrared-stimulated characteristics	907:961	rapid response near-infrared-stimulated characteristics	907:961	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	9	49	from	application	1831:1841	arg1	therapy					1853:1859	cancer therapy	1846:1859	cancer therapy	1846:1859	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	5	50	theme	near-infrared-stimulated	922:945	arg1	characteristics					947:961	rapid response near-infrared-stimulated characteristics	907:961	rapid response near-infrared-stimulated characteristics	907:961	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	10	51	dep	along	1952:1956	arg1	with					1958:1961	with	1958:1961	with	1958:1961	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	10	52	theme	composites	2072:2081	arg1	development					2038:2048	the development	2034:2048	the development of other inorganic 2D composites for applications in biomedicine	2034:2113	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	5	53	theme	cellulose/MXene	863:877	arg1	hydrogels					889:897	the cellulose/MXene composite hydrogels	859:897	the cellulose/MXene composite hydrogels	859:897	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	2	54	theme	drug	603:606	arg1	release					592:598	controlled release	581:598	controlled release of drug	581:606	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	54	theme	drug	603:606	arg1	conditions					543:552	physiological conditions	529:552	physiological conditions	529:552	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	2	54	theme	drug	603:606	arg1	capability					565:574	targeting capability	555:574	targeting capability	555:574	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	9	55	theme	prolonged	1627:1635	arg1	chemotherapy					1646:1657	prolonged adjuvant chemotherapy	1627:1657	prolonged adjuvant chemotherapy delivered using this nanoplatform	1627:1691	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	10	56	theme	inorganic	2059:2067	arg1	composites					2072:2081	other inorganic 2D composites	2053:2081	other inorganic 2D composites for applications in biomedicine	2053:2113	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	1	57	from	applications	281:292	arg1	PTT					330:332	PTT	330:332	PTT	330:332	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	1	57	from	applications	281:292	arg1	therapy					321:327	anticancer photothermal therapy	297:327	anticancer photothermal therapy (PTT)	297:333	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	3	58	theme	smart	643:647	arg1	nanoplatform					661:672	a smart MXene-based nanoplatform	641:672	a smart MXene-based nanoplatform for the treatment of cancer	641:700	Fabricating a smart MXene-based nanoplatform for the treatment of cancer therefore remains a challenge.
30058793	8	59	theme	intratumoral	1552:1563	arg1	injection					1565:1573	intratumoral injection	1552:1573	intratumoral injection	1552:1573	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	5	60	theme	dynamic	994:1000	arg1	process					1002:1008	a continuous dynamic process	981:1008	a continuous dynamic process in water	981:1017	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	8	61	theme	photothermal	1385:1396	arg1	performance					1398:1408	their excellent photothermal performance	1369:1408	their excellent photothermal performance	1369:1408	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	7	62	theme	three-dimensional	1261:1277	arg1	networks					1295:1302	the three-dimensional cellulose-based networks	1257:1302	the three-dimensional cellulose-based networks	1257:1302	This behavior is attributed to the expansion of the pores within the three-dimensional cellulose-based networks, triggered by illumination with an 808 nm light.
30058793	0	63	theme	Three-Dimensional	75:91	arg1	Nanoplatforms					101:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Smart Three-Dimensional Network Nanoplatforms	69:113	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	1	64	theme	photothermal	363:374	arg1	performance					376:386	their outstanding photothermal performance	345:386	their outstanding photothermal performance	345:386	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	6	65	theme	anticancer	1054:1063	arg1	doxorubicin					1070:1080	the anticancer drug doxorubicin	1050:1080	the anticancer drug doxorubicin hydrochloride (DOX)	1050:1100	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	6	65	theme	anticancer	1054:1063	arg1	DOX					1097:1099	DOX	1097:1099	DOX	1097:1099	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	2	66	theme	physiological	529:541	arg1	conditions					543:552	physiological conditions	529:552	physiological conditions	529:552	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	9	67	theme	adjuvant	1637:1644	arg1	chemotherapy					1646:1657	prolonged adjuvant chemotherapy	1627:1657	prolonged adjuvant chemotherapy delivered using this nanoplatform	1627:1691	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	6	68	dep	doxorubicin	1070:1080	arg1	hydrochloride					1082:1094	hydrochloride	1082:1094	the anticancer drug doxorubicin hydrochloride (DOX)	1050:1100	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	6	69	theme	DOX	1179:1181	arg1	release					1183:1189	the DOX release	1175:1189	the DOX release	1175:1189	As a result, when loaded with the anticancer drug doxorubicin hydrochloride (DOX), the cellulose/MXene hydrogels are capable of significantly accelerating the DOX release.
30058793	0	70	theme	Light-Induced	126:138	arg1	Swelling					140:147	Light-Induced Swelling	126:147	Light-Induced Swelling	126:147	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	5	71	contain	possess	899:905	arg1	hydrogels					889:897	the cellulose/MXene composite hydrogels	859:897	the cellulose/MXene composite hydrogels	859:897	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	5	71	contain	possess	899:905	arg2	characteristics					947:961	rapid response near-infrared-stimulated characteristics	907:961	rapid response near-infrared-stimulated characteristics	907:961	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	2	72	theme	inorganic	416:424	arg1	nanomaterials					429:441	the other inorganic 2D nanomaterials	406:441	the other inorganic 2D nanomaterials	406:441	However, as with the other inorganic 2D nanomaterials, the MXene-based nanoplatforms lack the appropriate biocompatibility and stability in physiological conditions, targeting capability, and controlled release of drug, for cancer therapy.
30058793	0	73	theme	MXene	16:20	arg1	Hydrogels					51:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels	0:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.	0:206	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	5	74	from	process	1002:1008	arg1	water					1013:1017	water	1013:1017	water	1013:1017	We have shown that the cellulose/MXene composite hydrogels possess rapid response near-infrared-stimulated characteristics, which present as a continuous dynamic process in water.
30058793	4	75	dep	cellulose	776:784	arg1	MXene					796:800	MXene	796:800	MXene	796:800	In this work, composite hydrogels based on cellulose and Ti3C2 MXene, were synthesized for the first time.
30058793	0	76	theme	Photothermal/Chemotherapy	161:185	arg1	Activity					198:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Bimodal Photothermal/Chemotherapy Anticancer Activity	153:205	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	1	77	dep	Two-dimensional	208:222	arg1	2D					225:226	2D	225:226	2D	225:226	Two-dimensional (2D) MXenes have recently been shown to be promising for applications in anticancer photothermal therapy (PTT), owing to their outstanding photothermal performance.
30058793	0	78	theme	Ti3C2	23:27	arg1	Hydrogels					51:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels	0:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.	0:206	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	10	79	theme	MXene-based	1924:1934	arg1	nanocomposites					1936:1949	smart MXene-based nanocomposites	1918:1949	smart MXene-based nanocomposites	1918:1949	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	0	80	theme	-Integrated	29:39	arg1	Hydrogels					51:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels	0:59	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.	0:206	Two-Dimensional MXene (Ti3C2)-Integrated Cellulose Hydrogels: Toward Smart Three-Dimensional Network Nanoplatforms Exhibiting Light-Induced Swelling and Bimodal Photothermal/Chemotherapy Anticancer Activity.
30058793	8	81	dep	controlled	1414:1423	arg1	sustained					1426:1434	sustained	1426:1434	sustained	1426:1434	Capitalizing on their excellent photothermal performance and controlled, sustained release of DOX, the cellulose/MXene hydrogels are utilized as a multifunctional nanoplatform for tumor treatment by intratumoral injection.
30058793	10	82	theme	promising	1969:1977	arg1	application					1979:1989	their promising application	1963:1989	their promising application against cancer	1963:2004	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	9	83	theme	instant	1718:1724	arg1	destruction					1732:1742	instant tumor destruction	1718:1742	instant tumor destruction	1718:1742	The results showed that the combination of PTT and prolonged adjuvant chemotherapy delivered using this nanoplatform was highly efficient for instant tumor destruction and for suppressing tumor relapse, demonstrating the potential of the nanoplatform for application in cancer therapy.
30058793	10	84	theme	smart	1918:1922	arg1	nanocomposites					1936:1949	smart MXene-based nanocomposites	1918:1949	smart MXene-based nanocomposites	1918:1949	Our work not only opens the door for the fabrication of smart MXene-based nanocomposites, along with their promising application against cancer, but also paves the way for the development of other inorganic 2D composites for applications in biomedicine.
30058793	7	85	theme	808	1339:1341	arg1	nm					1343:1344	nm	1343:1344	nm	1343:1344	This behavior is attributed to the expansion of the pores within the three-dimensional cellulose-based networks, triggered by illumination with an 808 nm light.
30058793	4	86	theme	first	828:832	arg1	time					834:837	the first time	824:837	the first time	824:837	In this work, composite hydrogels based on cellulose and Ti3C2 MXene, were synthesized for the first time.
30323560	1	0	with	phosphate	263:271	arg1	sulfate					320:326	SH sulfate	317:326	SH sulfate (SHS)	317:332	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	0	with	phosphate	263:271	arg1	SHS					329:331	SHS	329:331	SHS	329:331	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	0	with	phosphate	263:271	arg1	SH					310:311	SH	310:311	SH	310:311	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	0	with	phosphate	263:271	arg1	hyaluronate					297:307	sodium hyaluronate	290:307	sodium hyaluronate (SH)	290:312	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	4	1	theme	physicochemical	969:983	arg1	properties					985:994	the ultrastructural and physicochemical properties	945:994	the ultrastructural and physicochemical properties	945:994	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	10	2	theme	matrix	1802:1807	arg1	proteins					1815:1822	bone extracellular matrix (ECM) proteins	1783:1822	bone extracellular matrix (ECM) proteins	1783:1822	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	10	3	theme	extracellular	1788:1800	arg1	matrix					1802:1807	bone extracellular matrix	1783:1807	bone extracellular matrix (ECM) proteins	1783:1822	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	10	3	theme	extracellular	1788:1800	arg1	ECM					1810:1812	ECM	1810:1812	ECM	1810:1812	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	1	4	theme	material	413:420	arg1	composites					361:370	these composites	355:370	these composites	355:370	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	4	theme	material	413:420	arg1	type					393:396	a new type	387:396	a new type of bone repair material	387:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	8	5	theme	α-CSH/OCP/SH	1532:1543	arg1	group					1545:1549	the α-CSH/OCP/SH group	1528:1549	the α-CSH/OCP/SH group	1528:1549	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	12	6	from	promise	2238:2244	arg1	field					2253:2257	the field	2249:2257	the field of bone tissue engineering	2249:2284	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	3	7	dep	METHODS	565:571	arg1	combined					591:598	combined	591:598	combined α-CSH/OCP with SH or SHS	591:623	METHODS In this study, we combined α-CSH/OCP with SH or SHS.
30323560	7	8	theme	MPa	1388:1390	arg1	rate					1402:1405	a porous rate	1393:1405	a porous rate of 27.45%	1393:1415	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	8	theme	MPa	1388:1390	arg1	strength					1370:1377	a compressive strength	1356:1377	a compressive strength of 13.72 MPa	1356:1390	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	8	theme	MPa	1388:1390	arg1	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	2	9	theme	clinical	543:550	arg1	application					552:562	their clinical application	537:562	their clinical application	537:562	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	4	10	theme	electron	635:642	arg1	SEM					656:658	SEM	656:658	SEM	656:658	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	10	theme	electron	635:642	arg1	microscopy					644:653	Scanning electron microscopy	626:653	Scanning electron microscopy (SEM)	626:659	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	7	11	dep	RESULTS	1317:1323	arg1	had					1352:1354	had	1352:1354	had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%	1352:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	4	12	theme	in	838:839	arg1	degradation					847:857	in vitro degradation	838:857	in vitro degradation	838:857	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	2	13	theme	bone	511:514	arg1	materials					523:531	active bone repair materials	504:531	active bone repair materials	504:531	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	6	14	theme	differentiation	1174:1188	arg1	ability					1190:1196	the osteogenic differentiation ability	1159:1196	the osteogenic differentiation ability of these materials	1159:1215	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	10	15	theme	α-CSH/OCP/SH	1873:1884	arg1	group					1886:1890	the α-CSH/OCP/SH group	1869:1890	the α-CSH/OCP/SH group	1869:1890	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	1	16	theme	calcium	235:241	arg1	α-CSH/OCP					274:282	α-CSH/OCP	274:282	α-CSH/OCP	274:282	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	16	theme	calcium	235:241	arg1	phosphate					263:271	α-hemihydrate calcium sulfate/octacalcium phosphate	221:271	α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS)	221:332	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	9	17	theme	composite	1606:1614	arg1	results					1578:1584	The results	1574:1584	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1574:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	4	18	theme	Fourier-transform	662:678	arg1	spectroscopy					696:707	Fourier-transform infrared (FTIR) spectroscopy	662:707	Fourier-transform infrared (FTIR) spectroscopy	662:707	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	6	19	theme	blot	1253:1256	arg1	analysis					1258:1265	Western blot analysis	1245:1265	Western blot analysis	1245:1265	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	11	20	theme	model	1950:1954	arg1	XRD					1918:1920	XRD	1918:1920	XRD of the rabbit radius-defect model	1918:1954	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	12	21	theme	self-repair	2201:2211	arg1	induction					2166:2174	the induction	2162:2174	the induction of bone regeneration and self-repair	2162:2211	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	1	22	theme	bone	401:404	arg1	material					413:420	bone repair material	401:420	bone repair material	401:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	11	23	theme	bone	1971:1974	arg1	healing					1976:1982	bone healing	1971:1982	bone healing in the area implanted with α-CSH/OCP/SH	1971:2022	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	12	24	theme	bone	2262:2265	arg1	engineering					2274:2284	bone tissue engineering	2262:2284	bone tissue engineering	2262:2284	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	4	25	theme	composite	899:907	arg1	materials					909:917	these composite materials	893:917	these composite materials	893:917	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	7	26	contain	had	1352:1354	arg1	composite					1342:1350	The α-CSH/OCP/SH composite	1325:1350	The α-CSH/OCP/SH composite	1325:1350	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	26	contain	had	1352:1354	arg2	rate					1402:1405	a porous rate	1393:1405	a porous rate of 27.45%	1393:1415	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	26	contain	had	1352:1354	arg2	strength					1370:1377	a compressive strength	1356:1377	a compressive strength of 13.72 MPa	1356:1390	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	26	contain	had	1352:1354	arg2	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	4	27	theme	setting	824:830	arg1	time					832:835	setting time	824:835	setting time	824:835	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	0	28	theme	marrow-derived	99:112	arg1	osteogenesis					136:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	9	29	theme	pyrogenic	1650:1658	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	12	30	theme	regeneration	2184:2195	arg1	induction					2166:2174	the induction	2162:2174	the induction of bone regeneration and self-repair	2162:2211	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	0	31	theme	stem	126:129	arg1	osteogenesis					136:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	1	32	used	used	379:382	arg2	composites					361:370	these composites	355:370	these composites	355:370	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	32	used	used	379:382	arg2	type					393:396	a new type	387:396	a new type of bone repair material	387:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	4	33	theme	mechanical	868:877	arg1	properties					879:888	the mechanical properties	864:888	the mechanical properties of these composite materials	864:917	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	12	34	theme	clear	2142:2146	arg1	advantages					2148:2157	clear advantages	2142:2157	clear advantages in the induction of bone regeneration and self-repair	2142:2211	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	12	35	from	advantages	2148:2157	arg1	induction					2166:2174	the induction	2162:2174	the induction of bone regeneration and self-repair	2162:2211	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	9	36	theme	stimulation	1680:1690	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	0	37	theme	α-hemihydrate	0:12	arg1	phosphate					42:50	α-hemihydrate calcium sulfate/octacalcium phosphate	0:50	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate	0:83	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	5	38	theme	pyrogenic	1098:1106	arg1	tests					1139:1143	pyrogenic and intracutaneous stimulation tests	1098:1143	pyrogenic and intracutaneous stimulation tests	1098:1143	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	4	39	dep	infrared	680:687	arg1	FTIR					690:693	FTIR	690:693	FTIR	690:693	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	12	40	theme	good	2107:2110	arg1	biocompatibility					2112:2127	very good biocompatibility	2102:2127	very good biocompatibility	2102:2127	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	0	41	theme	sulfate/octacalcium	22:40	arg1	phosphate					42:50	α-hemihydrate calcium sulfate/octacalcium phosphate	0:50	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate	0:83	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	5	42	theme	intracutaneous	1112:1125	arg1	stimulation					1127:1137	intracutaneous stimulation	1112:1137	intracutaneous stimulation	1112:1137	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	2	43	theme	theoretical	448:458	arg1	basis					460:464	a theoretical basis	446:464	a theoretical basis	446:464	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	1	44	theme	SH	317:318	arg1	sulfate					320:326	SH sulfate	317:326	SH sulfate (SHS)	317:332	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	44	theme	SH	317:318	arg1	SHS					329:331	SHS	329:331	SHS	329:331	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	4	45	theme	materials	909:917	arg1	porosity					814:821	porosity	814:821	porosity	814:821	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	45	theme	materials	909:917	arg1	time					832:835	setting time	824:835	setting time	824:835	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	45	theme	materials	909:917	arg1	properties					879:888	the mechanical properties	864:888	the mechanical properties of these composite materials	864:917	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	45	theme	materials	909:917	arg1	degradation					847:857	in vitro degradation	838:857	in vitro degradation	838:857	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	7	46	theme	28-day	1426:1431	arg1	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	5	47	theme	acute	1078:1082	arg1	toxicity					1084:1091	acute toxicity	1078:1091	acute toxicity	1078:1091	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	2	48	theme	new	470:472	arg1	ideas					474:478	new ideas	470:478	new ideas	470:478	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	12	49	theme	CONCLUSION	2074:2083	arg1	α-CSH/OCP/SH					2085:2096	CONCLUSION α-CSH/OCP/SH	2074:2096	CONCLUSION α-CSH/OCP/SH	2074:2096	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	0	50	theme	sodium	66:71	arg1	hyaluronate					73:83	sodium hyaluronate	66:83	sodium hyaluronate	66:83	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	8	51	theme	group	1545:1549	arg1	%					1571:1571	greater than 90%	1556:1571	greater than 90%	1556:1571	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	8	51	theme	group	1545:1549	arg1	rates					1519:1523	the relative proliferation rates	1492:1523	the relative proliferation rates of the α-CSH/OCP/SH group	1492:1549	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	1	52	theme	sulfate/octacalcium	243:261	arg1	α-CSH/OCP					274:282	α-CSH/OCP	274:282	α-CSH/OCP	274:282	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	52	theme	sulfate/octacalcium	243:261	arg1	phosphate					263:271	α-hemihydrate calcium sulfate/octacalcium phosphate	221:271	α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS)	221:332	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	7	53	theme	%	1415:1415	arg1	rate					1402:1405	a porous rate	1393:1405	a porous rate of 27.45%	1393:1415	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	53	theme	%	1415:1415	arg1	strength					1370:1377	a compressive strength	1356:1377	a compressive strength of 13.72 MPa	1356:1390	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	53	theme	%	1415:1415	arg1	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	12	54	theme	tissue	2267:2272	arg1	engineering					2274:2284	bone tissue engineering	2262:2284	bone tissue engineering	2262:2284	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	8	55	theme	relative	1496:1503	arg1	%					1571:1571	greater than 90%	1556:1571	greater than 90%	1556:1571	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	8	55	theme	relative	1496:1503	arg1	rates					1519:1523	the relative proliferation rates	1492:1523	the relative proliferation rates of the α-CSH/OCP/SH group	1492:1549	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	6	56	theme	defect	1309:1314	arg1	model					1295:1299	an animal model	1285:1299	an animal model of bone defect	1285:1314	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	9	57	from	tests	1692:1696	arg1	results					1578:1584	The results	1574:1584	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1574:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	12	58	contain	has	2098:2100	arg1	α-CSH/OCP/SH					2085:2096	CONCLUSION α-CSH/OCP/SH	2074:2096	CONCLUSION α-CSH/OCP/SH	2074:2096	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	12	58	contain	has	2098:2100	arg2	biocompatibility					2112:2127	very good biocompatibility	2102:2127	very good biocompatibility	2102:2127	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	11	59	theme	rabbit	1929:1934	arg1	model					1950:1954	the rabbit radius-defect model	1925:1954	the rabbit radius-defect model	1925:1954	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	7	60	theme	porous	1395:1400	arg1	rate					1402:1405	a porous rate	1393:1405	a porous rate of 27.45%	1393:1415	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	10	61	theme	Western	1728:1734	arg1	analysis					1741:1748	Western blot analysis	1728:1748	Western blot analysis	1728:1748	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	10	62	theme	bone	1783:1786	arg1	matrix					1802:1807	bone extracellular matrix	1783:1807	bone extracellular matrix (ECM) proteins	1783:1822	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	10	62	theme	bone	1783:1786	arg1	ECM					1810:1812	ECM	1810:1812	ECM	1810:1812	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	8	63	theme	MTT	1462:1464	arg1	results					1472:1478	The MTT assay results	1458:1478	The MTT assay results	1458:1478	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	1	64	theme	sodium	290:295	arg1	SH					310:311	SH	310:311	SH	310:311	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	64	theme	sodium	290:295	arg1	hyaluronate					297:307	sodium hyaluronate	290:307	sodium hyaluronate (SH)	290:312	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	10	65	theme	proteins	1815:1822	arg1	expression					1769:1778	the expression	1765:1778	the expression of bone extracellular matrix (ECM) proteins	1765:1822	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	6	66	theme	materials	1207:1215	arg1	ability					1190:1196	the osteogenic differentiation ability	1159:1196	the osteogenic differentiation ability of these materials	1159:1215	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	7	67	theme	compressive	1358:1368	arg1	strength					1370:1377	a compressive strength	1356:1377	a compressive strength of 13.72 MPa	1356:1390	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	6	68	theme	bone	1304:1307	arg1	defect					1309:1314	bone defect	1304:1314	bone defect	1304:1314	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	1	69	theme	research	196:203	arg1	aims					183:186	The aims	179:186	The aims of this research	179:203	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	4	70	theme	wettability	777:787	arg1	test					789:792	the wettability test	773:792	the wettability test	773:792	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	71	theme	Scanning	626:633	arg1	SEM					656:658	SEM	656:658	SEM	656:658	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	71	theme	Scanning	626:633	arg1	microscopy					644:653	Scanning electron microscopy	626:653	Scanning electron microscopy (SEM)	626:659	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	2	72	theme	repair	516:521	arg1	materials					523:531	active bone repair materials	504:531	active bone repair materials	504:531	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	7	73	theme	α-CSH/OCP/SH	1329:1340	arg1	composite					1342:1350	The α-CSH/OCP/SH composite	1325:1350	The α-CSH/OCP/SH composite	1325:1350	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	1	74	theme	α-hemihydrate	221:233	arg1	α-CSH/OCP					274:282	α-CSH/OCP	274:282	α-CSH/OCP	274:282	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	74	theme	α-hemihydrate	221:233	arg1	phosphate					263:271	α-hemihydrate calcium sulfate/octacalcium phosphate	221:271	α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS)	221:332	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	9	75	theme	α-CSH/OCP/SH	1593:1604	arg1	composite					1606:1614	the α-CSH/OCP/SH composite	1589:1614	the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1589:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	6	76	theme	osteogenic	1163:1172	arg1	ability					1190:1196	the osteogenic differentiation ability	1159:1196	the osteogenic differentiation ability of these materials	1159:1215	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	1	77	theme	new	389:391	arg1	composites					361:370	these composites	355:370	these composites	355:370	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	1	77	theme	new	389:391	arg1	type					393:396	a new type	387:396	a new type of bone repair material	387:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	2	78	theme	materials	523:531	arg1	construction					488:499	the construction	484:499	the construction of active bone repair materials	484:531	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	2	78	theme	materials	523:531	arg1	application					552:562	their clinical application	537:562	their clinical application	537:562	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	6	79	theme	Western	1245:1251	arg1	analysis					1258:1265	Western blot analysis	1245:1265	Western blot analysis	1245:1265	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	9	80	theme	hemolysis	1623:1631	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	4	81	theme	infrared	680:687	arg1	spectroscopy					696:707	Fourier-transform infrared (FTIR) spectroscopy	662:707	Fourier-transform infrared (FTIR) spectroscopy	662:707	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	1	82	theme	repair	406:411	arg1	material					413:420	bone repair material	401:420	bone repair material	401:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	9	83	from	composite	1606:1614	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	9	84	theme	acute	1634:1638	arg1	toxicity					1640:1647	acute toxicity	1634:1647	acute toxicity	1634:1647	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	12	85	theme	bone	2179:2182	arg1	regeneration					2184:2195	bone regeneration	2179:2195	bone regeneration	2179:2195	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	12	86	theme	engineering	2274:2284	arg1	field					2253:2257	the field	2249:2257	the field of bone tissue engineering	2249:2284	CONCLUSION α-CSH/OCP/SH has very good biocompatibility and exhibits clear advantages in the induction of bone regeneration and self-repair, and this compound shows promise in the field of bone tissue engineering.
30323560	0	87	theme	mesenchymal	114:124	arg1	osteogenesis					136:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	11	88	theme	radius-defect	1936:1948	arg1	model					1950:1954	the rabbit radius-defect model	1925:1954	the rabbit radius-defect model	1925:1954	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	0	89	theme	cell	131:134	arg1	osteogenesis					136:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	9	90	from	results	1578:1584	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	11	91	dep	weeks	2054:2058	arg1	repair					2066:2071	repair	2066:2071	repair	2066:2071	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	4	92	theme	X-ray	710:714	arg1	diffraction					716:726	X-ray diffraction	710:726	X-ray diffraction (XRD)	710:732	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	92	theme	X-ray	710:714	arg1	XRD					729:731	XRD	729:731	XRD	729:731	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	10	93	theme	other	1904:1908	arg1	groups					1910:1915	the other groups	1900:1915	the other groups	1900:1915	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	6	94	theme	animal	1288:1293	arg1	model					1295:1299	an animal model	1285:1299	an animal model of bone defect	1285:1314	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	5	95	theme	composites	1042:1051	arg1	histocompatibility					1014:1031	the histocompatibility	1010:1031	the histocompatibility of these composites	1010:1051	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	7	96	theme	%	1455:1455	arg1	rate					1402:1405	a porous rate	1393:1405	a porous rate of 27.45%	1393:1415	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	96	theme	%	1455:1455	arg1	strength					1370:1377	a compressive strength	1356:1377	a compressive strength of 13.72 MPa	1356:1390	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	7	96	theme	%	1455:1455	arg1	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	4	97	dep	in	838:839	arg1	vitro					841:845	vitro	841:845	vitro	841:845	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	0	98	theme	calcium	14:20	arg1	phosphate					42:50	α-hemihydrate calcium sulfate/octacalcium phosphate	0:50	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate	0:83	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	5	99	theme	MTT	1056:1058	arg1	assay					1060:1064	MTT assay	1056:1064	MTT assay	1056:1064	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	4	100	theme	ultrastructural	949:963	arg1	properties					985:994	the ultrastructural and physicochemical properties	945:994	the ultrastructural and physicochemical properties	945:994	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	1	101	dep	PURPOSE	171:177	arg1	were					205:208	were	205:208	were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material	205:420	PURPOSE The aims of this research were to combine α-hemihydrate calcium sulfate/octacalcium phosphate (α-CSH/OCP) with sodium hyaluronate (SH) or SH sulfate (SHS) to determine whether these composites can be used as a new type of bone repair material.
30323560	5	102	theme	stimulation	1127:1137	arg1	tests					1139:1143	pyrogenic and intracutaneous stimulation tests	1098:1143	pyrogenic and intracutaneous stimulation tests	1098:1143	We evaluated the histocompatibility of these composites by MTT assay, hemolysis, acute toxicity, and pyrogenic and intracutaneous stimulation tests.
30323560	6	103	located	detected	1221:1228	arg2	ability					1190:1196	the osteogenic differentiation ability	1159:1196	the osteogenic differentiation ability of these materials	1159:1215	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	6	103	located	detected	1221:1228	arg1	addition					1149:1156	addition	1149:1156	addition	1149:1156	In addition, the osteogenic differentiation ability of these materials was detected in vitro using Western blot analysis and in vivo using an animal model of bone defect.
30323560	9	104	theme	normal	1714:1719	arg1	range					1721:1725	the normal range	1710:1725	the normal range	1710:1725	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	8	105	theme	assay	1466:1470	arg1	results					1472:1478	The MTT assay results	1458:1478	The MTT assay results	1458:1478	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	10	106	theme	blot	1736:1739	arg1	analysis					1741:1748	Western blot analysis	1728:1748	Western blot analysis	1728:1748	Western blot analysis indicated that the expression of bone extracellular matrix (ECM) proteins was notably upregulated and always higher in the α-CSH/OCP/SH group than in the other groups.
30323560	9	107	theme	intracutaneous	1665:1678	arg1	stimulation					1680:1690	intracutaneous stimulation	1665:1690	intracutaneous stimulation	1665:1690	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	11	108	from	healing	1976:1982	arg1	area					1991:1994	the area	1987:1994	the area implanted with α-CSH/OCP/SH	1987:2022	XRD of the rabbit radius-defect model indicated that bone healing in the area implanted with α-CSH/OCP/SH was excellent approximately 9 weeks after repair.
30323560	7	109	theme	degradation	1433:1443	arg1	rate					1445:1448	the 28-day degradation rate	1422:1448	the 28-day degradation rate of 64%	1422:1455	RESULTS The α-CSH/OCP/SH composite had a compressive strength of 13.72 MPa, a porous rate of 27.45%, and the 28-day degradation rate of 64%.
30323560	0	110	link	marrow-derived	99:112	arg1	osteogenesis					136:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	bone marrow-derived mesenchymal stem cell osteogenesis	94:147	α-hemihydrate calcium sulfate/octacalcium phosphate combined with sodium hyaluronate promotes bone marrow-derived mesenchymal stem cell osteogenesis in vitro and in vivo.
30323560	2	111	theme	active	504:509	arg1	materials					523:531	active bone repair materials	504:531	active bone repair materials	504:531	This study may provide a theoretical basis and new ideas for the construction of active bone repair materials and their clinical application.
30323560	9	112	theme	toxicity	1640:1647	arg1	tests					1692:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests	1619:1696	The results of the α-CSH/OCP/SH composite in the hemolysis, acute toxicity, pyrogenic, and intracutaneous stimulation tests were within the normal range.
30323560	8	113	theme	proliferation	1505:1517	arg1	%					1571:1571	greater than 90%	1556:1571	greater than 90%	1556:1571	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	8	113	theme	proliferation	1505:1517	arg1	rates					1519:1523	the relative proliferation rates	1492:1523	the relative proliferation rates of the α-CSH/OCP/SH group	1492:1549	The MTT assay results showed that the relative proliferation rates of the α-CSH/OCP/SH group were greater than 90%.
30323560	4	114	theme	thermogravimetric	735:751	arg1	TGA					763:765	TGA	763:765	TGA	763:765	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
30323560	4	114	theme	thermogravimetric	735:751	arg1	analysis					753:760	thermogravimetric analysis	735:760	thermogravimetric analysis (TGA)	735:766	Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and the wettability test were performed, and porosity, setting time, in vitro degradation, and the mechanical properties of these composite materials were analyzed to evaluate the ultrastructural and physicochemical properties.
29305219	5	0	theme	scaffolds	886:894	arg1	sizes					877:881	The average pore sizes	860:881	The average pore sizes of scaffolds	860:894	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	0	theme	scaffolds	886:894	arg1	%					970:970	porosity 71.72±3.46-91.17±2.42%	940:970	porosity 71.72±3.46-91.17±2.42%	940:970	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	1	dep	scaffolds	374:382	arg1	scaffolds					374:382	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	1	dep	scaffolds	374:382	arg1	cytocompatibility					434:450	cytocompatibility	434:450	cytocompatibility	434:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	1	dep	scaffolds	374:382	arg1	biostability					417:428	biostability	417:428	biostability	417:428	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	1	dep	scaffolds	374:382	arg1	morphology					384:393	morphology	384:393	morphology	384:393	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	1	dep	scaffolds	374:382	arg1	strength					407:414	mechanical strength	396:414	mechanical strength	396:414	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	2	contain	contained	1321:1329	arg2	collagen					1351:1358	fish collagen	1346:1358	fish collagen	1346:1358	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	2	contain	contained	1321:1329	arg2	amount					1336:1341	high amount	1331:1341	high amount of fish collagen excellently facilitated cell proliferation and adhesion	1331:1414	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	2	contain	contained	1321:1329	arg1	scaffolds					1311:1319	the scaffolds	1307:1319	the scaffolds	1307:1319	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	3	theme	In	1150:1151	arg1	culture					1159:1165	In vitro culture	1150:1165	In vitro culture of human fibroblasts and keratinocytes	1150:1204	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	5	4	dep	%	970:970	arg1	%					1147:1147	60.38±0.70-83.48±0.28%	1126:1147	60.38±0.70-83.48±0.28%	1126:1147	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	5	theme	various	186:192	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	7	6	contain	have	1488:1491	arg2	strength					1526:1533	mechanical strength	1515:1533	mechanical strength	1515:1533	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	6	contain	have	1488:1491	arg2	cytocompatibility					1567:1583	excellent cytocompatibility	1557:1583	excellent cytocompatibility	1557:1583	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	6	contain	have	1488:1491	arg1	amount					1444:1449	the high amount fish collagen and glycerin scaffolds	1435:1486	the high amount fish collagen and glycerin scaffolds	1435:1486	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	6	contain	have	1488:1491	arg2	porosity					1498:1505	high porosity	1493:1505	high porosity	1493:1505	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	7	theme	mechanical	1515:1524	arg1	strength					1526:1533	mechanical strength	1515:1533	mechanical strength	1515:1533	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	8	theme	high	1493:1496	arg1	porosity					1498:1505	high porosity	1493:1505	high porosity	1493:1505	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	9	dep	amount	1444:1449	arg1	collagen					1456:1463	fish collagen	1451:1463	fish collagen	1451:1463	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	7	9	dep	amount	1444:1449	arg1	scaffolds					1478:1486	glycerin scaffolds	1469:1486	glycerin scaffolds	1469:1486	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	1	10	theme	various	319:325	arg1	collagen/glycerin					353:369	various composition chitosan/fish collagen/glycerin	319:369	various composition chitosan/fish collagen/glycerin	319:369	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	7	11	theme	fish	1451:1454	arg1	collagen					1456:1463	fish collagen	1451:1463	fish collagen	1451:1463	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	1	12	theme	composition	327:337	arg1	collagen/glycerin					353:369	various composition chitosan/fish collagen/glycerin	319:369	various composition chitosan/fish collagen/glycerin	319:369	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	13	theme	mechanical	125:134	arg1	strength					136:143	mechanical strength	125:143	mechanical strength	125:143	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	14	theme	environment	1055:1065	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	15	theme	tensile	973:979	arg1	modulus					981:987	tensile modulus	973:987	tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa	973:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	4	16	theme	cytocompatibility	747:763	arg1	tests					765:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
29305219	3	17	theme	mechanical	636:645	arg1	tests					647:651	mechanical tests	636:651	mechanical tests	636:651	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	3	18	theme	various	527:533	arg1	compositions					535:546	various compositions	527:546	various compositions consisting in 3D scaffolds	527:573	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	1	19	theme	composition	194:204	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	20	theme	pore	872:875	arg1	sizes					877:881	The average pore sizes	860:881	The average pore sizes of scaffolds	860:894	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	20	theme	pore	872:875	arg1	%					970:970	porosity 71.72±3.46-91.17±2.42%	940:970	porosity 71.72±3.46-91.17±2.42%	940:970	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	6	21	theme	fish	1346:1349	arg1	collagen					1351:1358	fish collagen	1346:1358	fish collagen	1346:1358	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	1	22	theme	multicomponent	206:219	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	23	theme	3D	1257:1258	arg1	scaffolds					1260:1268	the various composition multicomponent 3D scaffolds	1218:1268	the various composition multicomponent 3D scaffolds	1218:1268	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	23	theme	3D	1257:1258	arg1	cytocompatibility					1280:1296	good cytocompatibility	1275:1296	good cytocompatibility	1275:1296	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	5	24	from	%	970:970	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	24	from	%	970:970	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	24	from	%	970:970	arg1	range					904:908	range	904:908	range of 100.73±27.62-116.01±52.06	904:937	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	25	theme	chitosan/fish	221:233	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	26	theme	porosity	940:947	arg1	sizes					877:881	The average pore sizes	860:881	The average pore sizes of scaffolds	860:894	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	26	theme	porosity	940:947	arg1	%					970:970	porosity 71.72±3.46-91.17±2.42%	940:970	porosity 71.72±3.46-91.17±2.42%	940:970	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	27	theme	chitosan/fish	339:351	arg1	collagen/glycerin					353:369	various composition chitosan/fish collagen/glycerin	319:369	various composition chitosan/fish collagen/glycerin	319:369	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	4	28	theme	engineering	835:845	arg1	application					847:857	tissue engineering application	828:857	tissue engineering application	828:857	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
29305219	5	29	theme	dry	992:994	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	30	theme	environment	996:1006	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	31	theme	collagen/glycerin	235:251	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	32	theme	composition	23:33	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	33	theme	collagen/glycerin	353:369	arg1	effect					309:314	the effect	305:314	the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility	305:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	34	theme	biodegradation	1094:1107	arg1	rate					1109:1112	biodegradation rate	1094:1112	biodegradation rate (at day 30)	1094:1124	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	6	35	theme	high	1331:1334	arg1	amount					1336:1341	high amount	1331:1341	high amount of fish collagen excellently facilitated cell proliferation and adhesion	1331:1414	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	35	theme	high	1331:1334	arg1	collagen					1351:1358	fish collagen	1346:1358	fish collagen	1346:1358	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	36	theme	multicomponent	1242:1255	arg1	scaffolds					1260:1268	the various composition multicomponent 3D scaffolds	1218:1268	the various composition multicomponent 3D scaffolds	1218:1268	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	36	theme	multicomponent	1242:1255	arg1	cytocompatibility					1280:1296	good cytocompatibility	1275:1296	good cytocompatibility	1275:1296	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	1	37	theme	3D	253:254	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	38	theme	various	15:21	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	39	theme	collagen	1351:1358	arg1	amount					1336:1341	high amount	1331:1341	high amount of fish collagen excellently facilitated cell proliferation and adhesion	1331:1414	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	39	theme	collagen	1351:1358	arg1	collagen					1351:1358	fish collagen	1346:1358	fish collagen	1346:1358	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	0	40	from	Effect	103:108	arg1	biostability					146:157	biostability	146:157	biostability	146:157	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	40	from	Effect	103:108	arg1	morphology					113:122	morphology	113:122	morphology	113:122	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	40	from	Effect	103:108	arg1	strength					136:143	mechanical strength	125:143	mechanical strength	125:143	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	40	from	Effect	103:108	arg1	cytocompatibility					163:179	cytocompatibility	163:179	cytocompatibility	163:179	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	41	theme	wet	1051:1053	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	1	42	theme	porous	256:261	arg1	scaffolds					263:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	182:271	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	0	43	theme	chitosan/fish	50:62	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	44	from	effect	309:314	arg1	scaffolds					374:382	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	44	from	effect	309:314	arg1	cytocompatibility					434:450	cytocompatibility	434:450	cytocompatibility	434:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	44	from	effect	309:314	arg1	biostability					417:428	biostability	417:428	biostability	417:428	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	44	from	effect	309:314	arg1	morphology					384:393	morphology	384:393	morphology	384:393	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	44	from	effect	309:314	arg1	strength					407:414	mechanical strength	396:414	mechanical strength	396:414	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	7	45	theme	high	1439:1442	arg1	amount					1444:1449	the high amount fish collagen and glycerin scaffolds	1435:1486	the high amount fish collagen and glycerin scaffolds	1435:1486	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	3	46	theme	compositions	535:546	arg1	effects					516:522	The effects	512:522	The effects of various compositions consisting in 3D scaffolds	512:573	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	3	47	theme	3D	562:563	arg1	scaffolds					565:573	3D scaffolds	562:573	3D scaffolds	562:573	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	0	48	theme	multicomponent	35:48	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	49	from	modulus	981:987	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	49	from	modulus	981:987	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	49	from	modulus	981:987	arg1	range					904:908	range	904:908	range of 100.73±27.62-116.01±52.06	904:937	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	6	50	theme	composition	1230:1240	arg1	scaffolds					1260:1268	the various composition multicomponent 3D scaffolds	1218:1268	the various composition multicomponent 3D scaffolds	1218:1268	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	50	theme	composition	1230:1240	arg1	cytocompatibility					1280:1296	good cytocompatibility	1275:1296	good cytocompatibility	1275:1296	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	0	51	theme	3D	82:83	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	5	52	theme	tensile	1032:1038	arg1	modulus					1040:1046	tensile modulus	1032:1046	tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa	1032:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	53	theme	100.73±27.62-116.01±52.06	913:937	arg1	range					904:908	range	904:908	range of 100.73±27.62-116.01±52.06	904:937	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	7	54	theme	excellent	1557:1565	arg1	cytocompatibility					1567:1583	excellent cytocompatibility	1557:1583	excellent cytocompatibility	1557:1583	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	4	55	theme	tissue	828:833	arg1	application					847:857	tissue engineering application	828:857	tissue engineering application	828:857	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
29305219	0	56	theme	collagen/glycerin	64:80	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	57	theme	mechanical	396:405	arg1	scaffolds					374:382	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	scaffolds morphology, mechanical strength, biostability and cytocompatibility	374:450	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	1	57	theme	mechanical	396:405	arg1	strength					407:414	mechanical strength	396:414	mechanical strength	396:414	The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility.
29305219	3	58	theme	scaffolds	686:694	arg1	morphology					672:681	the morphology	668:681	the morphology of scaffolds	668:694	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	7	59	theme	glycerin	1469:1476	arg1	scaffolds					1478:1486	glycerin scaffolds	1469:1486	glycerin scaffolds	1469:1486	It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.
29305219	3	60	from	effect	658:663	arg1	morphology					672:681	the morphology	668:681	the morphology of scaffolds	668:694	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	6	61	theme	cell	1384:1387	arg1	proliferation					1389:1401	cell proliferation	1384:1401	cell proliferation	1384:1401	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	5	62	from	modulus	1040:1046	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	62	from	modulus	1040:1046	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	62	from	modulus	1040:1046	arg1	range					904:908	range	904:908	range of 100.73±27.62-116.01±52.06	904:937	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	63	from	day	1118:1120	arg1	modulus					981:987	tensile modulus	973:987	tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa	973:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	63	from	day	1118:1120	arg1	rate					1109:1112	biodegradation rate	1094:1112	biodegradation rate (at day 30)	1094:1124	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	63	from	day	1118:1120	arg1	modulus					1040:1046	tensile modulus	1032:1046	tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa	1032:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	63	from	day	1118:1120	arg1	sizes					877:881	The average pore sizes	860:881	The average pore sizes of scaffolds	860:894	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	63	from	day	1118:1120	arg1	%					970:970	porosity 71.72±3.46-91.17±2.42%	940:970	porosity 71.72±3.46-91.17±2.42%	940:970	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	64	theme	average	864:870	arg1	sizes					877:881	The average pore sizes	860:881	The average pore sizes of scaffolds	860:894	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	64	theme	average	864:870	arg1	%					970:970	porosity 71.72±3.46-91.17±2.42%	940:970	porosity 71.72±3.46-91.17±2.42%	940:970	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	6	65	theme	various	1222:1228	arg1	scaffolds					1260:1268	the various composition multicomponent 3D scaffolds	1218:1268	the various composition multicomponent 3D scaffolds	1218:1268	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	65	theme	various	1222:1228	arg1	cytocompatibility					1280:1296	good cytocompatibility	1275:1296	good cytocompatibility	1275:1296	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	3	66	theme	FT-IR	597:601	arg1	analysis					603:610	FT-IR analysis	597:610	FT-IR analysis	597:610	The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically.
29305219	5	67	from	rate	1109:1112	arg1	0.32±0.03-0.14±0.04MPa					1067:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	wet environment 0.32±0.03-0.14±0.04MPa	1051:1088	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	67	from	rate	1109:1112	arg1	1.47±0.08-0.17±0.03MPa					1008:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	dry environment 1.47±0.08-0.17±0.03MPa	992:1029	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	5	67	from	rate	1109:1112	arg1	range					904:908	range	904:908	range of 100.73±27.62-116.01±52.06	904:937	The average pore sizes of scaffolds were in range of 100.73±27.62-116.01±52.06, porosity 71.72±3.46-91.17±2.42%, tensile modulus in dry environment 1.47±0.08-0.17±0.03MPa, tensile modulus in wet environment 0.32±0.03-0.14±0.04MPa and biodegradation rate (at day 30) 60.38±0.70-83.48±0.28%.
29305219	6	68	theme	fibroblasts	1176:1186	arg1	culture					1159:1165	In vitro culture	1150:1165	In vitro culture of human fibroblasts and keratinocytes	1150:1204	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	0	69	theme	scaffolds	92:100	arg1	Development					0:10	Development	0:10	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.	0:180	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	70	theme	keratinocytes	1192:1204	arg1	culture					1159:1165	In vitro culture	1150:1165	In vitro culture of human fibroblasts and keratinocytes	1150:1204	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	4	71	used	used	776:779	arg2	tests					765:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
29305219	4	72	theme	scaffolds	807:815	arg1	ability					796:802	the ability	792:802	the ability of scaffolds to use for tissue engineering application	792:857	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
29305219	2	73	theme	freeze-drying	487:499	arg1	technique					501:509	freeze-drying technique	487:509	freeze-drying technique	487:509	The scaffolds were fabricated via freeze-drying technique.
29305219	6	74	theme	human	1170:1174	arg1	fibroblasts					1176:1186	human fibroblasts	1170:1186	human fibroblasts	1170:1186	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	0	75	theme	porous	85:90	arg1	scaffolds					92:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds	15:100	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	76	dep	In	1150:1151	arg1	vitro					1153:1157	vitro	1153:1157	vitro	1153:1157	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	0	77	dep	Development	0:10	arg1	Effect					103:108	Effect	103:108	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.	0:180	Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility.
29305219	6	78	theme	good	1275:1278	arg1	scaffolds					1260:1268	the various composition multicomponent 3D scaffolds	1218:1268	the various composition multicomponent 3D scaffolds	1218:1268	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	6	78	theme	good	1275:1278	arg1	cytocompatibility					1280:1296	good cytocompatibility	1275:1296	good cytocompatibility	1275:1296	In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion.
29305219	4	79	theme	biostability	730:741	arg1	tests					765:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests	726:769	The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application.
30031820	0	0	theme	graphene	81:88	arg1	composite					105:113	graphene oxide hydrogel composite	81:113	graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution	81:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	1	1	theme	graft	272:276	arg1	hydrogel					288:295	graft copolymer hydrogel	272:295	graft copolymer hydrogel	272:295	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	2	2	theme	optimum	646:652	arg1	hydrogel					654:661	an optimum hydrogel	643:661	an optimum hydrogel	643:661	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	3	3	theme	diffraction	809:819	arg1	XRD					831:833	XRD	831:833	XRD	831:833	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	3	theme	diffraction	809:819	arg1	microscopy					889:898	Scanning electron microscopy	871:898	Scanning electron microscopy (SEM)	871:904	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	3	theme	diffraction	809:819	arg1	analysis					821:828	X-ray diffraction analysis	803:828	X-ray diffraction analysis (XRD)	803:834	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	3	theme	diffraction	809:819	arg1	analysis					855:862	Thermogravimetric analysis	837:862	Thermogravimetric analysis (TGA)	837:868	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	1	4	theme	acrylic	326:332	arg1	acid					334:337	acrylic acid	326:337	acrylic acid (AA)	326:342	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	4	theme	acrylic	326:332	arg1	AA					340:341	AA	340:341	AA	340:341	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	5	theme	acid	334:337	arg1	copolymerization					306:321	graft copolymerization	300:321	graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	300:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	0	6	theme	aqueous	171:177	arg1	solution					179:186	aqueous solution	171:186	aqueous solution	171:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	3	7	dep	transform	775:783	arg1	FT-IR					795:799	FT-IR	795:799	FT-IR	795:799	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	7	dep	transform	775:783	arg1	infrared					785:792	infrared	785:792	infrared	785:792	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	2	8	from	degree	617:622	arg1	media					633:637	water media	627:637	water media	627:637	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	3	9	dep	structure	929:937	arg1	the					925:927	the	925:927	the	925:927	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	0	10	theme	hydrogel	96:103	arg1	composite					105:113	graphene oxide hydrogel composite	81:113	graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution	81:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	5	11	theme	adsorption	1274:1283	arg1	process					1285:1291	the adsorption process	1270:1291	the adsorption process	1270:1291	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	3	12	theme	thermal	940:946	arg1	stability					948:956	thermal stability	940:956	thermal stability	940:956	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	1	13	theme	N	396:396	arg1	presence					384:391	the presence	380:391	the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	380:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	4	14	theme	methylene	1046:1054	arg1	adsorption					1009:1018	The adsorption	1005:1018	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results	1005:1101	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	5	15	theme	adsorption	1385:1394	arg1	Qmax					1406:1409	Qmax	1406:1409	Qmax	1406:1409	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	15	theme	adsorption	1385:1394	arg1	capacity					1396:1403	a maximum adsorption capacity	1375:1403	a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively	1375:1479	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	0	16	theme	oxide	90:94	arg1	composite					105:113	graphene oxide hydrogel composite	81:113	graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution	81:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	3	17	theme	Scanning	871:878	arg1	microscopy					889:898	Scanning electron microscopy	871:898	Scanning electron microscopy (SEM)	871:904	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	17	theme	Scanning	871:878	arg1	analysis					821:828	X-ray diffraction analysis	803:828	X-ray diffraction analysis (XRD)	803:834	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	17	theme	Scanning	871:878	arg1	SEM					901:903	SEM	901:903	SEM	901:903	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	5	18	theme	rate	1326:1329	arg1	model					1331:1335	the pseudo-second-order rate model	1302:1335	the pseudo-second-order rate model	1302:1335	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	6	19	dep	benign	1528:1533	arg1	recoverable/recyclable					1544:1565	recoverable/recyclable	1544:1565	recoverable/recyclable	1544:1565	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	1	20	theme	cross-linking	466:478	arg1	method					232:237	facile and efficient method	211:237	facile and efficient method	211:237	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	20	theme	cross-linking	466:478	arg1	agent					480:484	a cross-linking agent	464:484	a cross-linking agent	464:484	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	5	21	theme	pseudo-second-order	1306:1324	arg1	model					1331:1335	the pseudo-second-order rate model	1302:1335	the pseudo-second-order rate model	1302:1335	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	2	22	theme	graphene	700:707	arg1	rGO					716:718	rGO	716:718	rGO	716:718	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	2	22	theme	graphene	700:707	arg1	oxide					709:713	reduced graphene oxide	692:713	reduced graphene oxide (rGO)	692:719	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	1	23	theme	N'-Methylenebisacrylamide	398:422	arg1	presence					384:391	the presence	380:391	the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	380:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	6	24	theme	benign	1528:1533	arg1	material					1567:1574	environmentally benign, readily recoverable/recyclable material	1512:1574	environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal	1512:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	2	25	theme	reduced	692:698	arg1	rGO					716:718	rGO	716:718	rGO	716:718	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	2	25	theme	reduced	692:698	arg1	oxide					709:713	reduced graphene oxide	692:713	reduced graphene oxide (rGO)	692:719	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	4	26	theme	adsorbent	1185:1193	arg1	loading					1195:1201	adsorbent loading	1185:1201	adsorbent loading	1185:1201	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	2	27	theme	hydrogel	564:571	arg1	optimization					548:559	The optimization	544:559	The optimization of hydrogel	544:571	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	5	28	from	25 °C	1447:1451	arg1	Qmax					1406:1409	Qmax	1406:1409	Qmax	1406:1409	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	28	from	25 °C	1447:1451	arg1	1052.63 mg/g					1415:1426	1052.63 mg/g	1415:1426	1052.63 mg/g	1415:1426	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	28	from	25 °C	1447:1451	arg1	capacity					1396:1403	a maximum adsorption capacity	1375:1403	a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively	1375:1479	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	28	from	25 °C	1447:1451	arg1	793.65 mg/g					1432:1442	793.65 mg/g	1432:1442	793.65 mg/g	1432:1442	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	4	29	theme	blue	1056:1059	arg1	methylene					1046:1054	methylene blue	1046:1059	methylene blue (MB)	1046:1064	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	4	29	theme	blue	1056:1059	arg1	MB					1062:1063	MB	1062:1063	MB	1062:1063	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	0	30	theme	assisted	10:17	arg1	synthesis					19:27	Microwave assisted synthesis	0:27	Microwave assisted synthesis of xanthan	0:38	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	1	31	theme	xanthan	349:355	arg1	biopolymer					366:375	xanthan gum (XG) biopolymer	349:375	xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	349:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	32	from	biopolymer	366:375	arg1	presence					384:391	the presence	380:391	the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	380:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	3	33	used	used	911:914	arg2	Fourier					767:773	The Fourier	763:773	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM)	763:904	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	0	34	theme	Microwave	0:8	arg1	synthesis					19:27	Microwave assisted synthesis	0:27	Microwave assisted synthesis of xanthan	0:38	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	6	35	theme	dyes	1630:1633	arg1	removal					1635:1641	dyes removal	1630:1641	dyes removal	1630:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	3	36	theme	XG-cl-pAA	976:984	arg1	morphology					962:971	morphology	962:971	morphology	962:971	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	36	theme	XG-cl-pAA	976:984	arg1	structure					929:937	structure	929:937	structure	929:937	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	36	theme	XG-cl-pAA	976:984	arg1	stability					948:956	thermal stability	940:956	thermal stability	940:956	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	2	37	theme	swelling	608:615	arg1	degree					617:622	the maximum swelling degree	596:622	the maximum swelling degree in water media	596:637	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	4	38	theme	violet	1030:1035	arg1	adsorption					1009:1018	The adsorption	1005:1018	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results	1005:1101	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	2	39	theme	maximum	600:606	arg1	degree					617:622	the maximum swelling degree	596:622	the maximum swelling degree in water media	596:637	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	0	40	from	solution	179:186	arg1	adsorption					119:128	adsorption	119:128	adsorption of methylene blue and methyl violet from aqueous solution	119:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	5	41	theme	1052.63 mg/g	1415:1426	arg1	Qmax					1406:1409	Qmax	1406:1409	Qmax	1406:1409	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	41	theme	1052.63 mg/g	1415:1426	arg1	capacity					1396:1403	a maximum adsorption capacity	1375:1403	a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively	1375:1479	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	1	42	theme	gum	357:359	arg1	biopolymer					366:375	xanthan gum (XG) biopolymer	349:375	xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	349:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	4	43	from	study	1071:1075	arg1	results					1095:1101	results	1095:1101	results	1095:1101	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	4	43	from	study	1071:1075	arg1	mode					1086:1089	batch mode	1080:1089	batch mode	1080:1089	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	1	44	theme	present	196:202	arg1	study					204:208	the present study	192:208	the present study	192:208	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	45	theme	copolymer	278:286	arg1	hydrogel					288:295	graft copolymer hydrogel	272:295	graft copolymer hydrogel	272:295	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	0	46	theme	blue	143:146	arg1	methylene					133:141	methylene blue	133:146	methylene blue	133:146	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	5	47	theme	Langmuir	1341:1348	arg1	adsorption					1350:1359	Langmuir adsorption	1341:1359	Langmuir adsorption	1341:1359	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	3	48	theme	Thermogravimetric	837:853	arg1	TGA					865:867	TGA	865:867	TGA	865:867	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	48	theme	Thermogravimetric	837:853	arg1	analysis					855:862	Thermogravimetric analysis	837:862	Thermogravimetric analysis (TGA)	837:868	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	48	theme	Thermogravimetric	837:853	arg1	analysis					821:828	X-ray diffraction analysis	803:828	X-ray diffraction analysis (XRD)	803:834	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	0	49	theme	xanthan	32:38	arg1	synthesis					19:27	Microwave assisted synthesis	0:27	Microwave assisted synthesis of xanthan	0:38	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	1	50	theme	hydrogel	288:295	arg1	synthesis					259:267	the synthesis	255:267	the synthesis of graft copolymer hydrogel	255:295	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	0	51	theme	methylene	133:141	arg1	adsorption					119:128	adsorption	119:128	adsorption of methylene blue and methyl violet from aqueous solution	119:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	1	52	theme	XG	362:363	arg1	biopolymer					366:375	xanthan gum (XG) biopolymer	349:375	xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	349:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	5	53	from	capacity	1396:1403	arg1	25 °C					1447:1451	25 °C	1447:1451	25 °C	1447:1451	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	54	theme	adsorption	1244:1253	arg1	potential					1255:1263	a very high adsorption potential	1232:1263	a very high adsorption potential	1232:1263	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	6	55	with	material	1567:1574	arg1	capacity					1602:1609	excellent adsorption capacity	1581:1609	excellent adsorption capacity for application in dyes removal	1581:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	0	56	theme	acrylic	53:59	arg1	acid					61:64	acrylic acid	53:64	acrylic acid	53:64	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	4	57	theme	methyl	1023:1028	arg1	violet					1030:1035	methyl violet	1023:1035	methyl violet (MV)	1023:1040	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	4	57	theme	methyl	1023:1028	arg1	MV					1038:1039	MV	1038:1039	MV	1038:1039	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	0	58	theme	methyl	152:157	arg1	violet					159:164	methyl violet	152:164	methyl violet	152:164	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	4	59	theme	contact	1153:1159	arg1	time					1161:1164	contact time	1153:1164	contact time	1153:1164	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	0	60	dep	gum-cl-poly	40:50	arg1	acid					61:64	acrylic acid	53:64	acrylic acid	53:64	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	1	61	theme	ammonium	435:442	arg1	persulfate					444:453	ammonium persulfate	435:453	ammonium persulfate	435:453	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	5	62	theme	793.65 mg/g	1432:1442	arg1	Qmax					1406:1409	Qmax	1406:1409	Qmax	1406:1409	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	62	theme	793.65 mg/g	1432:1442	arg1	capacity					1396:1403	a maximum adsorption capacity	1375:1403	a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively	1375:1479	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	1	63	theme	graft	300:304	arg1	copolymerization					306:321	graft copolymerization	300:321	graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	300:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	4	64	theme	dependent	1127:1135	arg1	adsorption					1109:1118	adsorption	1109:1118	adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading	1109:1201	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	3	65	theme	XG-cl-pAA/rGO	990:1002	arg1	morphology					962:971	morphology	962:971	morphology	962:971	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	65	theme	XG-cl-pAA/rGO	990:1002	arg1	structure					929:937	structure	929:937	structure	929:937	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	65	theme	XG-cl-pAA/rGO	990:1002	arg1	stability					948:956	thermal stability	940:956	thermal stability	940:956	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	1	66	theme	persulfate	444:453	arg1	presence					384:391	the presence	380:391	the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS)	380:459	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	5	67	theme	maximum	1377:1383	arg1	Qmax					1406:1409	Qmax	1406:1409	Qmax	1406:1409	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	5	67	theme	maximum	1377:1383	arg1	capacity					1396:1403	a maximum adsorption capacity	1375:1403	a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively	1375:1479	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	1	68	theme	microwave	521:529	arg1	irradiation					531:541	microwave irradiation	521:541	microwave irradiation	521:541	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	4	69	theme	batch	1080:1084	arg1	mode					1086:1089	batch mode	1080:1089	batch mode	1080:1089	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	2	70	theme	water	627:631	arg1	media					633:637	water media	627:637	water media	627:637	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	5	71	theme	high	1239:1242	arg1	potential					1255:1263	a very high adsorption potential	1232:1263	a very high adsorption potential	1232:1263	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	2	72	theme	XG-cl-pAA/rGO	729:741	arg1	composite					752:760	XG-cl-pAA/rGO hydrogel composite	729:760	XG-cl-pAA/rGO hydrogel composite	729:760	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	1	73	theme	facile	211:216	arg1	initiator					490:498	initiator	490:498	initiator	490:498	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	73	theme	facile	211:216	arg1	method					232:237	facile and efficient method	211:237	facile and efficient method	211:237	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	73	theme	facile	211:216	arg1	agent					480:484	a cross-linking agent	464:484	a cross-linking agent	464:484	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	0	74	theme	violet	159:164	arg1	adsorption					119:128	adsorption	119:128	adsorption of methylene blue and methyl violet from aqueous solution	119:186	Microwave assisted synthesis of xanthan gum-cl-poly (acrylic acid) based-reduced graphene oxide hydrogel composite for adsorption of methylene blue and methyl violet from aqueous solution.
30031820	4	75	theme	solution	1140:1147	arg1	pH					1149:1150	solution pH	1140:1150	solution pH	1140:1150	The adsorption of methyl violet (MV) and methylene blue (MB) were study in batch mode and results shows adsorption highly dependent on solution pH, contact time, concentration and adsorbent loading.
30031820	6	76	theme	adsorption	1591:1600	arg1	capacity					1602:1609	excellent adsorption capacity	1581:1609	excellent adsorption capacity for application in dyes removal	1581:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	3	77	dep	Fourier	767:773	arg1	transform					775:783	transform	775:783	transform	775:783	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	5	78	dep	model	1331:1335	arg1	isotherm					1361:1368	isotherm	1361:1368	isotherm	1361:1368	The XG-cl-pAA/rGO exhibited a very high adsorption potential, and the adsorption process followed the pseudo-second-order rate model and Langmuir adsorption isotherm with a maximum adsorption capacity (Qmax) of 1052.63 mg/g and 793.65 mg/g at 25 °C for MV and MB, respectively.
30031820	1	79	theme	efficient	222:230	arg1	initiator					490:498	initiator	490:498	initiator	490:498	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	79	theme	efficient	222:230	arg1	method					232:237	facile and efficient method	211:237	facile and efficient method	211:237	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	1	79	theme	efficient	222:230	arg1	agent					480:484	a cross-linking agent	464:484	a cross-linking agent	464:484	In the present study, facile and efficient method was adopted for the synthesis of graft copolymer hydrogel by graft copolymerization of acrylic acid (AA) onto xanthan gum (XG) biopolymer in the presence of N,N'-Methylenebisacrylamide (MBA), and ammonium persulfate (APS) as a cross-linking agent and initiator, respectively, under microwave irradiation.
30031820	2	80	theme	hydrogel	743:750	arg1	composite					752:760	XG-cl-pAA/rGO hydrogel composite	729:760	XG-cl-pAA/rGO hydrogel composite	729:760	The optimization of hydrogel were selected based on the maximum swelling degree in water media and an optimum hydrogel was further impregnated with reduced graphene oxide (rGO) to form XG-cl-pAA/rGO hydrogel composite.
30031820	6	81	theme	excellent	1581:1589	arg1	capacity					1602:1609	excellent adsorption capacity	1581:1609	excellent adsorption capacity for application in dyes removal	1581:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30031820	3	82	theme	electron	880:887	arg1	microscopy					889:898	Scanning electron microscopy	871:898	Scanning electron microscopy (SEM)	871:904	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	82	theme	electron	880:887	arg1	analysis					821:828	X-ray diffraction analysis	803:828	X-ray diffraction analysis (XRD)	803:834	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	82	theme	electron	880:887	arg1	SEM					901:903	SEM	901:903	SEM	901:903	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	83	theme	X-ray	803:807	arg1	XRD					831:833	XRD	831:833	XRD	831:833	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	83	theme	X-ray	803:807	arg1	microscopy					889:898	Scanning electron microscopy	871:898	Scanning electron microscopy (SEM)	871:904	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	83	theme	X-ray	803:807	arg1	analysis					821:828	X-ray diffraction analysis	803:828	X-ray diffraction analysis (XRD)	803:834	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	3	83	theme	X-ray	803:807	arg1	analysis					855:862	Thermogravimetric analysis	837:862	Thermogravimetric analysis (TGA)	837:868	The Fourier transform infrared (FT-IR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA), Scanning electron microscopy (SEM) were used to study the structure, thermal stability and morphology of XG-cl-pAA and XG-cl-pAA/rGO.
30031820	6	84	from	application	1615:1625	arg1	removal					1635:1641	dyes removal	1630:1641	dyes removal	1630:1641	We recommend XG-cl-pAA/rGO as environmentally benign, readily recoverable/recyclable material with excellent adsorption capacity for application in dyes removal.
30832883	9	0	theme	elastomers	1155:1164	arg1	properties					1131:1140	the properties	1127:1140	the properties of the green elastomers	1127:1164	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	1	1	theme	high	141:144	arg1	elastomer					158:166	a green, high performance elastomer	132:166	elastomer	158:166	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	1	theme	high	141:144	arg1	polyurethane					111:122	Waterborne polyurethane	100:122	Waterborne polyurethane (PU)	100:127	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	4	2	theme	good	595:598	arg1	printability					600:611	the good printability	591:611	the good printability	591:611	Rheological measurements supported the good printability.
30832883	5	3	theme	TEM	614:616	arg1	images					618:623	TEM images	614:623	TEM images	614:623	TEM images revealed that CNFs linked multiple PU nanoparticles to form a 'skewer' structure.
30832883	1	4	theme	performance	146:156	arg1	elastomer					158:166	a green, high performance elastomer	132:166	elastomer	158:166	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	4	theme	performance	146:156	arg1	polyurethane					111:122	Waterborne polyurethane	100:122	Waterborne polyurethane (PU)	100:127	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	7	5	theme	viscosity	896:904	arg1	PEO					916:918	PEO	916:918	PEO	916:918	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	7	5	theme	viscosity	896:904	arg1	enhancer					906:913	a water-soluble viscosity enhancer	880:913	a water-soluble viscosity enhancer (PEO)	880:919	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	1	6	theme	three-dimensional	236:252	arg1	printing					259:266	direct three-dimensional (3D) printing	229:266	direct three-dimensional (3D) printing	229:266	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	3	7	theme	in-situ	537:543	arg1	synthesis					545:553	in-situ synthesis	537:553	in-situ synthesis	537:553	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	3	8	theme	agent	524:528	arg1	amount					501:506	the amount	497:506	the amount of neutralizing agent	497:528	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	3	8	theme	agent	524:528	arg1	agent					524:528	neutralizing agent	511:528	neutralizing agent	511:528	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	7	9	theme	water-soluble	882:894	arg1	PEO					916:918	PEO	916:918	PEO	916:918	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	7	9	theme	water-soluble	882:894	arg1	enhancer					906:913	a water-soluble viscosity enhancer	880:913	a water-soluble viscosity enhancer (PEO)	880:919	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	8	10	from	scaffolds	960:968	arg1	proliferating					939:951	proliferating	939:951	proliferating	939:951	Fibroblasts kept proliferating in the scaffolds for two weeks.
30832883	6	11	theme	PU/CNF	707:712	arg1	scaffolds					714:722	PU/CNF scaffolds	707:722	PU/CNF scaffolds	707:722	PU/CNF scaffolds were 3D-printed with excellent pattern fidelity and structure stability.
30832883	5	12	theme	multiple	651:658	arg1	nanoparticles					663:675	multiple PU nanoparticles	651:675	multiple PU nanoparticles	651:675	TEM images revealed that CNFs linked multiple PU nanoparticles to form a 'skewer' structure.
30832883	9	13	theme	PU	1089:1090	arg1	viscosity					1065:1073	the viscosity	1061:1073	the viscosity of waterborne PU for direct 3D printing	1061:1113	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	3	14	theme	cellulose	421:429	arg1	CNFs					444:447	CNFs	444:447	CNFs	444:447	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	3	14	theme	cellulose	421:429	arg1	nanofibrils					431:441	cellulose nanofibrils	421:441	cellulose nanofibrils (CNFs)	421:448	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	4	15	theme	Rheological	556:566	arg1	measurements					568:579	Rheological measurements	556:579	Rheological measurements	556:579	Rheological measurements supported the good printability.
30832883	5	16	theme	PU	660:661	arg1	nanoparticles					663:675	multiple PU nanoparticles	651:675	multiple PU nanoparticles	651:675	TEM images revealed that CNFs linked multiple PU nanoparticles to form a 'skewer' structure.
30832883	1	17	theme	Waterborne	100:109	arg1	PU					125:126	PU	125:126	PU	125:126	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	17	theme	Waterborne	100:109	arg1	polyurethane					111:122	Waterborne polyurethane	100:122	Waterborne polyurethane (PU)	100:127	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	17	theme	Waterborne	100:109	arg1	elastomer					158:166	a green, high performance elastomer	132:166	elastomer	158:166	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	8	18	from	proliferating	939:951	arg1	scaffolds					960:968	the scaffolds	956:968	the scaffolds for two weeks	956:982	Fibroblasts kept proliferating in the scaffolds for two weeks.
30832883	3	19	theme	printable	355:363	arg1	composites					368:377	printable PU composites	355:377	printable PU composites	355:377	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	2	20	theme	additional	286:295	arg1	properties					297:306	additional properties	286:306	additional properties	286:306	Composite brings additional properties while reinforcing the substrate.
30832883	9	21	theme	direct	1096:1101	arg1	printing					1106:1113	direct 3D printing	1096:1113	direct 3D printing	1096:1113	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	3	22	theme	PU	365:366	arg1	composites					368:377	printable PU composites	355:377	printable PU composites	355:377	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
30832883	7	23	theme	compression	812:822	arg1	modulus					824:830	the compression modulus	808:830	the compression modulus	808:830	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	7	23	theme	compression	812:822	arg1	higher					841:846	higher	841:846	higher	841:846	Meanwhile, the compression modulus was much higher than the scaffolds printed with a water-soluble viscosity enhancer (PEO).
30832883	9	24	theme	3D	1103:1104	arg1	printing					1106:1113	direct 3D printing	1096:1113	direct 3D printing	1096:1113	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	0	25	theme	cellulose	42:50	arg1	nanofibers					52:61	cellulose nanofibers	42:61	cellulose nanofibers	42:61	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	1	26	theme	dispersion	193:202	arg1	low					221:223	low	221:223	low	221:223	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	26	theme	dispersion	193:202	arg1	viscosity					176:184	the viscosity	172:184	the viscosity of the dispersion	172:202	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	0	27	theme	polyurethane	25:36	arg1	Composites					0:9	Composites	0:9	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.	0:98	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	0	27	theme	polyurethane	25:36	arg1	polyurethane					25:36	waterborne polyurethane	14:36	waterborne polyurethane	14:36	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	0	27	theme	polyurethane	25:36	arg1	nanofibers					52:61	cellulose nanofibers	42:61	cellulose nanofibers	42:61	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	6	28	theme	structure	776:784	arg1	stability					786:794	structure stability	776:794	structure stability	776:794	PU/CNF scaffolds were 3D-printed with excellent pattern fidelity and structure stability.
30832883	0	29	theme	nanofibers	52:61	arg1	Composites					0:9	Composites	0:9	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.	0:98	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	0	29	theme	nanofibers	52:61	arg1	polyurethane					25:36	waterborne polyurethane	14:36	waterborne polyurethane	14:36	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	0	29	theme	nanofibers	52:61	arg1	nanofibers					52:61	cellulose nanofibers	42:61	cellulose nanofibers	42:61	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	5	30	theme	skewer	688:693	arg1	structure					696:704	a 'skewer' structure	685:704	a 'skewer' structure	685:704	TEM images revealed that CNFs linked multiple PU nanoparticles to form a 'skewer' structure.
30832883	9	31	theme	novel	1032:1036	arg1	way					1049:1051	a novel and unique way	1030:1051	a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers	1030:1164	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	6	32	theme	pattern	755:761	arg1	fidelity					763:770	excellent pattern fidelity	745:770	excellent pattern fidelity	745:770	PU/CNF scaffolds were 3D-printed with excellent pattern fidelity and structure stability.
30832883	9	33	theme	waterborne	1078:1087	arg1	PU					1089:1090	waterborne PU	1078:1090	waterborne PU	1078:1090	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	1	34	dep	three-dimensional	236:252	arg1	3D					255:256	3D	255:256	3D	255:256	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	6	35	theme	excellent	745:753	arg1	fidelity					763:770	excellent pattern fidelity	745:770	excellent pattern fidelity	745:770	PU/CNF scaffolds were 3D-printed with excellent pattern fidelity and structure stability.
30832883	1	36	theme	green	134:138	arg1	elastomer					158:166	a green, high performance elastomer	132:166	elastomer	158:166	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	1	36	theme	green	134:138	arg1	polyurethane					111:122	Waterborne polyurethane	100:122	Waterborne polyurethane (PU)	100:127	Waterborne polyurethane (PU) is a green, high performance elastomer but the viscosity of the dispersion is generally too low for direct three-dimensional (3D) printing.
30832883	9	37	theme	unique	1042:1047	arg1	way					1049:1051	a novel and unique way	1030:1051	a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers	1030:1164	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	0	38	theme	3D	67:68	arg1	printing					70:77	3D printing	67:77	3D printing	67:77	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	0	39	theme	waterborne	14:23	arg1	polyurethane					25:36	waterborne polyurethane	14:36	waterborne polyurethane	14:36	Composites of waterborne polyurethane and cellulose nanofibers for 3D printing and bioapplications.
30832883	9	40	theme	green	1149:1153	arg1	elastomers					1155:1164	the green elastomers	1145:1164	the green elastomers	1145:1164	The interaction between CNF and PU may offer a novel and unique way to tune the viscosity of waterborne PU for direct 3D printing and enhance the properties of the green elastomers.
30832883	3	41	theme	neutralizing	511:522	arg1	agent					524:528	neutralizing agent	511:528	neutralizing agent	511:528	In the study, printable PU composites were successfully prepared by introducing cellulose nanofibrils (CNFs) and the viscosity was effectively regulated by the amount of neutralizing agent during in-situ synthesis.
29456005	1	0	theme	GH	136:137	arg1	BsCsn46A					124:131	BsCsn46A	124:131	BsCsn46A	124:131	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	1	0	theme	GH	136:137	arg1	chitosanase					149:159	a GH family 46 chitosanase	134:159	a GH family 46 chitosanase	134:159	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	6	1	theme	chitosanases	929:940	arg1	chitosanases					929:940	the fastest chitosanases	917:940	the fastest chitosanases described so far	917:957	Importantly, BsCsn46A seems to be one of the fastest chitosanases described so far.
29456005	6	1	theme	chitosanases	929:940	arg1	one					910:912	one	910:912	one	910:912	Importantly, BsCsn46A seems to be one of the fastest chitosanases described so far.
29456005	1	2	theme	family	139:144	arg1	BsCsn46A					124:131	BsCsn46A	124:131	BsCsn46A	124:131	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	1	2	theme	family	139:144	arg1	chitosanase					149:159	a GH family 46 chitosanase	134:159	a GH family 46 chitosanase	134:159	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	6	3	theme	fastest	921:927	arg1	chitosanases					929:940	the fastest chitosanases	917:940	the fastest chitosanases described so far	917:957	Importantly, BsCsn46A seems to be one of the fastest chitosanases described so far.
29456005	3	4	theme	non-processive	617:630	arg1	endo-mode					632:640	a non-processive endo-mode	615:640	a non-processive endo-mode of action	615:650	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	7	5	theme	hexameric	1040:1048	arg1	substrate					1050:1058	a hexameric substrate	1038:1058	a hexameric substrate	1038:1058	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	3	6	theme	1H	482:483	arg1	NMR					485:487	1H NMR	482:487	1H NMR	482:487	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	7	7	contain	has	1060:1062	arg1	substrate					1050:1058	a hexameric substrate	1038:1058	a hexameric substrate	1038:1058	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	7	7	contain	has	1060:1062	arg2	modes					1089:1093	three productive binding modes	1064:1093	three productive binding modes occurring with similar frequencies	1064:1128	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	1	8	theme	chitosan	249:256	arg1	bioconversion					232:244	bioconversion	232:244	bioconversion of chitosan to chito-oligosaccharides (CHOS)	232:289	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	7	9	theme	similar	1110:1116	arg1	frequencies					1118:1128	similar frequencies	1110:1128	similar frequencies	1110:1128	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	3	10	theme	action	645:650	arg1	endo-mode					632:640	a non-processive endo-mode	615:640	a non-processive endo-mode of action	615:650	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	2	11	theme	action	347:352	arg1	mode					339:342	the mode	335:342	the mode of action of this enzyme	335:367	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	2	11	theme	action	347:352	arg1	composition					377:387	the composition	373:387	the composition of its products	373:403	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	2	12	theme	products	396:403	arg1	mode					339:342	the mode	335:342	the mode of action of this enzyme	335:367	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	2	12	theme	products	396:403	arg1	composition					377:387	the composition	373:387	the composition of its products	373:403	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	3	13	theme	mass	494:497	arg1	spectrometry					499:510	mass spectrometry	494:510	mass spectrometry	494:510	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	4	14	theme	reaction	770:777	arg1	time					779:782	the reaction time	766:782	the reaction time	766:782	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	2	15	theme	mode	339:342	arg1	analysis					318:325	in-depth analysis	309:325	in-depth analysis of both the mode of action of this enzyme and the composition of its products	309:403	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	7	16	theme	modes	1008:1012	arg1	analysis					973:980	The detailed analysis	960:980	The detailed analysis of preferred endo-binding modes using H218O	960:1024	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	4	17	theme	mixtures	684:691	arg1	composition					657:667	The composition	653:667	The composition of the product mixtures	653:691	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	2	18	theme	composition	377:387	arg1	analysis					318:325	in-depth analysis	309:325	in-depth analysis of both the mode of action of this enzyme and the composition of its products	309:403	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	4	19	theme	product	676:682	arg1	mixtures					684:691	the product mixtures	672:691	the product mixtures	672:691	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	5	20	theme	product	806:812	arg1	profiles					814:821	product profiles	806:821	product profiles	806:821	Detailed analysis of product profiles revealed differences compared to other chitosanases.
29456005	7	21	theme	preferred	985:993	arg1	modes					1008:1012	preferred endo-binding modes	985:1012	preferred endo-binding modes using H218O	985:1024	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	2	22	theme	enzyme	362:367	arg1	action					347:352	action	347:352	action of this enzyme	347:367	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	7	23	theme	endo-binding	995:1006	arg1	modes					1008:1012	preferred endo-binding modes	985:1012	preferred endo-binding modes using H218O	985:1024	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	1	24	contain	have	213:216	arg1	BsCsn46A					124:131	BsCsn46A	124:131	BsCsn46A	124:131	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	1	24	contain	have	213:216	arg2	potential					218:226	potential	218:226	potential for bioconversion of chitosan to chito-oligosaccharides (CHOS)	218:289	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	1	24	contain	have	213:216	arg1	chitosanase					149:159	a GH family 46 chitosanase	134:159	a GH family 46 chitosanase	134:159	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	7	25	theme	productive	1070:1079	arg1	modes					1089:1093	three productive binding modes	1064:1093	three productive binding modes occurring with similar frequencies	1064:1128	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	4	26	theme	chitosan	753:760	arg1	acetylation					734:744	acetylation	734:744	acetylation of the chitosan	734:760	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	2	27	theme	in-depth	309:316	arg1	analysis					318:325	in-depth analysis	309:325	in-depth analysis of both the mode of action of this enzyme and the composition of its products	309:403	However, so far, in-depth analysis of both the mode of action of this enzyme and the composition of its products were lacking.
29456005	7	28	theme	binding	1081:1087	arg1	modes					1089:1093	three productive binding modes	1064:1093	three productive binding modes occurring with similar frequencies	1064:1128	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	1	29	from	subtilis	175:182	arg1	BsCsn46A					124:131	BsCsn46A	124:131	BsCsn46A	124:131	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	1	29	from	subtilis	175:182	arg1	chitosanase					149:159	a GH family 46 chitosanase	134:159	a GH family 46 chitosanase	134:159	BsCsn46A, a GH family 46 chitosanase from Bacillus subtilis had been previously shown to have potential for bioconversion of chitosan to chito-oligosaccharides (CHOS).
29456005	0	30	from	subtilis	103:110	arg1	chitosanase					77:87	family 46 chitosanase	67:87	family 46 chitosanase from Bacillus subtilis (BsCsn46A)	67:121	Bioconversion of chitosan into chito-oligosaccharides (CHOS) using family 46 chitosanase from Bacillus subtilis (BsCsn46A).
29456005	4	31	theme	acetylation	734:744	arg1	degree					724:729	the degree	720:729	the degree of acetylation of the chitosan	720:760	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	4	31	theme	acetylation	734:744	arg1	time					779:782	the reaction time	766:782	the reaction time	766:782	The composition of the product mixtures can be tailored by varying the degree of acetylation of the chitosan and the reaction time.
29456005	5	32	theme	other	856:860	arg1	chitosanases					862:873	other chitosanases	856:873	other chitosanases	856:873	Detailed analysis of product profiles revealed differences compared to other chitosanases.
29456005	7	33	theme	detailed	964:971	arg1	analysis					973:980	The detailed analysis	960:980	The detailed analysis of preferred endo-binding modes using H218O	960:1024	The detailed analysis of preferred endo-binding modes using H218O showed that a hexameric substrate has three productive binding modes occurring with similar frequencies.
29456005	3	34	theme	acetylation	588:598	arg1	degrees					600:606	acetylation degrees	588:606	acetylation degrees	588:606	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	5	35	theme	profiles	814:821	arg1	analysis					794:801	Detailed analysis	785:801	Detailed analysis of product profiles	785:821	Detailed analysis of product profiles revealed differences compared to other chitosanases.
29456005	0	36	theme	family	67:72	arg1	chitosanase					77:87	family 46 chitosanase	67:87	family 46 chitosanase from Bacillus subtilis (BsCsn46A)	67:121	Bioconversion of chitosan into chito-oligosaccharides (CHOS) using family 46 chitosanase from Bacillus subtilis (BsCsn46A).
29456005	3	37	theme	degrees	600:606	arg1	wide-variety					572:583	a wide-variety	570:583	a wide-variety of acetylation degrees	570:606	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	3	38	theme	size	451:454	arg1	chromatography					466:479	size exclusion chromatography	451:479	size exclusion chromatography	451:479	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29456005	5	39	theme	Detailed	785:792	arg1	analysis					794:801	Detailed analysis	785:801	Detailed analysis of product profiles	785:821	Detailed analysis of product profiles revealed differences compared to other chitosanases.
29456005	0	40	theme	chitosan	17:24	arg1	Bioconversion					0:12	Bioconversion	0:12	Bioconversion of chitosan into chito-oligosaccharides (CHOS)	0:59	Bioconversion of chitosan into chito-oligosaccharides (CHOS) using family 46 chitosanase from Bacillus subtilis (BsCsn46A).
29456005	3	41	theme	exclusion	456:464	arg1	chromatography					466:479	size exclusion chromatography	451:479	size exclusion chromatography	451:479	In this study, we have employed size exclusion chromatography, 1H NMR, and mass spectrometry to reveal that BsCsn46A can rapidly cleave chitosans with a wide-variety of acetylation degrees, using a non-processive endo-mode of action.
29736498	10	0	theme	wide	1512:1515	arg1	applicability					1517:1529	wide applicability	1512:1529	wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis	1512:1600	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	4	1	theme	properties	500:509	arg1	range					491:495	A wide range	484:495	A wide range of properties	484:509	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	10	2	contain	have	1507:1510	arg1	preparations					1490:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	10	2	contain	have	1507:1510	arg2	applicability					1517:1529	wide applicability	1512:1529	wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis	1512:1600	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	8	3	theme	guar	1307:1310	arg1	gum					1312:1314	guar gum	1307:1314	guar gum	1307:1314	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	8	4	theme	corrosion	1259:1267	arg1	resistance					1269:1278	the corrosion resistance	1255:1278	the corrosion resistance of saliva substitutes with guar gum and poloxamer 407	1255:1332	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	4	5	theme	tension	588:594	arg1	changes					548:554	changes	548:554	changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients	548:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	6	dep	changes	548:554	arg1	h					622:622	1, 24, 96, 168 h	607:622	changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients	548:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	7	theme	corrosion	821:829	arg1	potential					810:818	open circuit potential	797:818	open circuit potential	797:818	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	7	theme	corrosion	821:829	arg1	potential					831:839	corrosion potential	821:839	corrosion potential	821:839	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	1	8	theme	natural	193:199	arg1	saliva					201:206	natural saliva	193:206	natural saliva	193:206	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
29736498	9	9	theme	human	1457:1461	arg1	saliva					1469:1474	human whole saliva	1457:1474	human whole saliva	1457:1474	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	4	10	theme	surface	580:586	arg1	tension					588:594	surface tension	580:594	surface tension	580:594	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	3	11	theme	plasma-treated	385:398	arg1	PBSPT					407:411	PBSPT	407:411	PBSPT	407:411	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	3	11	theme	plasma-treated	385:398	arg1	water					400:404	plasma-treated water	385:404	plasma-treated water (PBSPT)	385:412	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	4	12	dep	physicochemical	531:545	arg1	changes					548:554	changes	548:554	changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients	548:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	13	theme	polarisation	842:853	arg1	potential					810:818	open circuit potential	797:818	open circuit potential	797:818	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	13	theme	polarisation	842:853	arg1	resistance					855:864	polarisation resistance	842:864	polarisation resistance	842:864	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	14	theme	friction	725:732	arg1	coefficient					710:720	coefficient	710:720	coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple	710:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	5	15	theme	friction	950:957	arg1	viscosity					924:932	viscosity	924:932	viscosity	924:932	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	15	theme	friction	950:957	arg1	pH					920:921	pH	920:921	pH	920:921	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	15	theme	friction	950:957	arg1	parameters					973:982	corrosion parameters	963:982	corrosion parameters similar to those of natural saliva	963:1017	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	15	theme	friction	950:957	arg1	coefficient					935:945	coefficient	935:945	coefficient of friction	935:957	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	10	16	theme	e.g.	1549:1552	arg1	xerostomia					1554:1563	e.g. xerostomia	1549:1563	e.g. xerostomia	1549:1563	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	4	17	theme	wide	486:489	arg1	range					491:495	A wide range	484:495	A wide range of properties	484:509	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	18	dep	rheological	651:661	arg1	viscoelasticity					678:692	viscoelasticity	678:692	viscoelasticity	678:692	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	18	dep	rheological	651:661	arg1	viscosity					664:672	viscosity	664:672	viscosity	664:672	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	6	19	theme	viscoelasticity	1064:1078	arg1	values					1043:1048	the values	1039:1048	the values of viscosity, viscoelasticity and the coefficient of friction	1039:1110	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	1	20	theme	mucin-based	135:145	arg1	saliva					147:152	artificial mucin-based saliva	124:152	artificial mucin-based saliva	124:152	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
29736498	4	21	theme	circuit	802:808	arg1	potential					810:818	open circuit potential	797:818	open circuit potential	797:818	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	21	theme	circuit	802:808	arg1	potential					831:839	corrosion potential	821:839	corrosion potential	821:839	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	21	theme	circuit	802:808	arg1	resistance					855:864	polarisation resistance	842:864	polarisation resistance	842:864	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	22	theme	titanium	738:745	arg1	Ti-6Al-7Nb					753:762	titanium alloy Ti-6Al-7Nb	738:762	titanium alloy Ti-6Al-7Nb kinematic couple	738:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	3	23	theme	guar	457:460	arg1	gum					462:464	guar gum	457:464	guar gum	457:464	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	5	24	theme	RESULTS	868:874	arg1	preparations					883:894	RESULTS Saliva preparations	868:894	RESULTS Saliva preparations based only on mucin	868:914	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	0	25	theme	Physicochemical	45:59	arg1	Properties					78:87	- Physicochemical and Tribological Properties	43:87	- Physicochemical and Tribological Properties	43:87	Artificial Mucin-based Saliva Preparations - Physicochemical and Tribological Properties.
29736498	3	26	theme	gum	462:464	arg1	addition					419:426	addition	419:426	addition of porcine gastric mucin and guar gum or poloxamer 407	419:481	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	0	27	theme	Mucin-based	11:21	arg1	Saliva					23:28	Artificial Mucin-based Saliva	0:28	Artificial Mucin-based Saliva	0:28	Artificial Mucin-based Saliva Preparations - Physicochemical and Tribological Properties.
29736498	9	28	dep	CONCLUSION	1335:1344	arg1	compositions					1363:1374	the tested compositions	1352:1374	the tested compositions	1352:1374	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	7	29	theme	preparations	1213:1224	arg1	tension					1195:1201	the surface tension	1183:1201	the surface tension of tested preparations	1183:1224	Similar results were obtained for poloxamer 407, whereas it decreased the surface tension of tested preparations.
29736498	3	30	theme	mucin	447:451	arg1	addition					419:426	addition	419:426	addition of porcine gastric mucin and guar gum or poloxamer 407	419:481	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	0	31	theme	Artificial	0:9	arg1	Saliva					23:28	Artificial Mucin-based Saliva	0:28	Artificial Mucin-based Saliva	0:28	Artificial Mucin-based Saliva Preparations - Physicochemical and Tribological Properties.
29736498	9	32	contain	have	1430:1433	arg1	mucin					1410:1414	mucin	1410:1414	mucin	1410:1414	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	9	32	contain	have	1430:1433	arg2	parameters					1435:1444	parameters	1435:1444	parameters similar to human whole saliva	1435:1474	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	4	33	theme	mixing	630:635	arg1	ingredients					637:647	mixing ingredients	630:647	mixing ingredients	630:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	34	theme	pH	559:560	arg1	changes					548:554	changes	548:554	changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients	548:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	5	35	theme	Saliva	876:881	arg1	preparations					883:894	RESULTS Saliva preparations	868:894	RESULTS Saliva preparations based only on mucin	868:914	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	6	36	theme	friction	1103:1110	arg1	viscosity					1053:1061	viscosity	1053:1061	viscosity	1053:1061	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	6	36	theme	friction	1103:1110	arg1	viscoelasticity					1064:1078	viscoelasticity	1064:1078	viscoelasticity	1064:1078	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	6	36	theme	friction	1103:1110	arg1	coefficient					1088:1098	the coefficient	1084:1098	the coefficient of friction	1084:1110	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	5	37	contain	had	916:918	arg2	coefficient					935:945	coefficient	935:945	coefficient of friction	935:957	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	37	contain	had	916:918	arg1	preparations					883:894	RESULTS Saliva preparations	868:894	RESULTS Saliva preparations based only on mucin	868:914	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	37	contain	had	916:918	arg2	viscosity					924:932	viscosity	924:932	viscosity	924:932	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	37	contain	had	916:918	arg2	pH					920:921	pH	920:921	pH	920:921	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	5	37	contain	had	916:918	arg2	parameters					973:982	corrosion parameters	963:982	corrosion parameters similar to those of natural saliva	963:1017	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	8	38	theme	Plasma-treated	1227:1240	arg1	PBS					1242:1244	Plasma-treated PBS	1227:1244	Plasma-treated PBS	1227:1244	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	3	39	theme	phosphate	322:330	arg1	saline					341:346	phosphate buffered saline	322:346	phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT)	322:412	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	3	39	theme	phosphate	322:330	arg1	PBS					349:351	PBS	349:351	PBS	349:351	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	1	40	theme	artificial	124:133	arg1	saliva					147:152	artificial mucin-based saliva	124:152	artificial mucin-based saliva	124:152	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
29736498	4	41	dep	corrosive	786:794	arg1	potential					810:818	open circuit potential	797:818	open circuit potential	797:818	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	41	dep	corrosive	786:794	arg1	potential					831:839	corrosion potential	821:839	corrosion potential	821:839	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	41	dep	corrosive	786:794	arg1	resistance					855:864	polarisation resistance	842:864	polarisation resistance	842:864	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	3	42	theme	porcine	431:437	arg1	mucin					447:451	porcine gastric mucin	431:451	porcine gastric mucin	431:451	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	4	43	theme	conductivity	563:574	arg1	changes					548:554	changes	548:554	changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients	548:647	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	8	44	dep	saliva	1283:1288	arg1	substitutes					1290:1300	substitutes	1290:1300	substitutes	1290:1300	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	6	45	theme	Guar	1020:1023	arg1	gum					1025:1027	Guar gum	1020:1027	Guar gum	1020:1027	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	9	46	theme	similar	1446:1452	arg1	parameters					1435:1444	parameters	1435:1444	parameters similar to human whole saliva	1435:1474	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	3	47	theme	gastric	439:445	arg1	mucin					447:451	porcine gastric mucin	431:451	porcine gastric mucin	431:451	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	7	48	theme	Similar	1113:1119	arg1	results					1121:1127	Similar results	1113:1127	Similar results	1113:1127	Similar results were obtained for poloxamer 407, whereas it decreased the surface tension of tested preparations.
29736498	8	49	theme	saliva	1283:1288	arg1	resistance					1269:1278	the corrosion resistance	1255:1278	the corrosion resistance of saliva substitutes with guar gum and poloxamer 407	1255:1332	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	5	50	theme	similar	984:990	arg1	parameters					973:982	corrosion parameters	963:982	corrosion parameters similar to those of natural saliva	963:1017	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	3	51	dep	Saliva	287:292	arg1	substitutes					294:304	substitutes	294:304	substitutes	294:304	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	4	52	dep	characterised	516:528	arg1	rheological					651:661	rheological	651:661	rheological	651:661	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	52	dep	characterised	516:528	arg1	physicochemical					531:545	physicochemical	531:545	physicochemical	531:545	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	52	dep	characterised	516:528	arg1	corrosive					786:794	corrosive	786:794	corrosive	786:794	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	52	dep	characterised	516:528	arg1	tribological					696:707	tribological	696:707	tribological	696:707	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	3	53	theme	deionised	366:374	arg1	water					376:380	deionised water	366:380	deionised water	366:380	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	2	54	theme	saliva	254:259	arg1	preparations					261:272	six saliva preparations	250:272	six saliva preparations	250:272	MATERIALS AND METHODS Natural saliva and six saliva preparations were tested.
29736498	10	55	theme	saliva	1483:1488	arg1	preparations					1490:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	1	56	dep	PURPOSE	90:96	arg1	investigate					112:122	investigate	112:122	investigate artificial mucin-based saliva	112:152	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
29736498	1	56	dep	PURPOSE	90:96	arg1	create					101:106	create	101:106	create	101:106	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
29736498	10	57	theme	metal	1585:1589	arg1	prosthesis					1591:1600	a metal prosthesis	1583:1600	a metal prosthesis	1583:1600	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	10	58	with	patients	1535:1542	arg1	patients					1568:1575	patients	1568:1575	patients using a metal prosthesis	1568:1600	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	10	58	with	patients	1535:1542	arg1	xerostomia					1554:1563	e.g. xerostomia	1549:1563	e.g. xerostomia	1549:1563	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	4	59	theme	open	797:800	arg1	potential					810:818	open circuit potential	797:818	open circuit potential	797:818	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	59	theme	open	797:800	arg1	potential					831:839	corrosion potential	821:839	corrosion potential	821:839	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	4	59	theme	open	797:800	arg1	resistance					855:864	polarisation resistance	842:864	polarisation resistance	842:864	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	9	60	dep	substitutes	1384:1394	arg1	based					1396:1400	based	1396:1400	substitutes based only on mucin were found to have parameters similar to human whole saliva	1384:1474	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	9	61	theme	whole	1463:1467	arg1	saliva					1469:1474	human whole saliva	1457:1474	human whole saliva	1457:1474	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	5	62	theme	corrosion	963:971	arg1	parameters					973:982	corrosion parameters	963:982	corrosion parameters similar to those of natural saliva	963:1017	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	3	63	theme	buffered	332:339	arg1	saline					341:346	phosphate buffered saline	322:346	phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT)	322:412	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	3	63	theme	buffered	332:339	arg1	PBS					349:351	PBS	349:351	PBS	349:351	Saliva substitutes were made using phosphate buffered saline (PBS) prepared in deionised water or plasma-treated water (PBSPT) with addition of porcine gastric mucin and guar gum or poloxamer 407.
29736498	5	64	theme	natural	1004:1010	arg1	saliva					1012:1017	natural saliva	1004:1017	natural saliva	1004:1017	RESULTS Saliva preparations based only on mucin had pH, viscosity, coefficient of friction and corrosion parameters similar to those of natural saliva.
29736498	4	65	dep	tribological	696:707	arg1	coefficient					710:720	coefficient	710:720	coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple	710:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	9	66	theme	tested	1356:1361	arg1	compositions					1363:1374	the tested compositions	1352:1374	the tested compositions	1352:1374	CONCLUSION Among the tested compositions, saliva substitutes based only on mucin were found to have parameters similar to human whole saliva.
29736498	2	67	dep	Natural	231:237	arg1	saliva					239:244	saliva	239:244	saliva	239:244	MATERIALS AND METHODS Natural saliva and six saliva preparations were tested.
29736498	10	68	theme	Mucin	1477:1481	arg1	preparations					1490:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations	1477:1501	Mucin saliva preparations may have wide applicability for patients with e.g. xerostomia or patients using a metal prosthesis.
29736498	2	69	theme	Natural	231:237	arg1	MATERIALS					209:217	MATERIALS	209:217	MATERIALS	209:217	MATERIALS AND METHODS Natural saliva and six saliva preparations were tested.
29736498	4	70	theme	alloy	747:751	arg1	Ti-6Al-7Nb					753:762	titanium alloy Ti-6Al-7Nb	738:762	titanium alloy Ti-6Al-7Nb kinematic couple	738:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	6	71	theme	viscosity	1053:1061	arg1	values					1043:1048	the values	1039:1048	the values of viscosity, viscoelasticity and the coefficient of friction	1039:1110	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	6	72	theme	coefficient	1088:1098	arg1	values					1043:1048	the values	1039:1048	the values of viscosity, viscoelasticity and the coefficient of friction	1039:1110	Guar gum increased the values of viscosity, viscoelasticity and the coefficient of friction.
29736498	7	73	theme	surface	1187:1193	arg1	tension					1195:1201	the surface tension	1183:1201	the surface tension of tested preparations	1183:1224	Similar results were obtained for poloxamer 407, whereas it decreased the surface tension of tested preparations.
29736498	8	74	with	saliva	1283:1288	arg1	poloxamer					1320:1328	poloxamer 407	1320:1332	poloxamer 407	1320:1332	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	8	74	with	saliva	1283:1288	arg1	gum					1312:1314	guar gum	1307:1314	guar gum	1307:1314	Plasma-treated PBS enhanced the corrosion resistance of saliva substitutes with guar gum and poloxamer 407.
29736498	7	75	theme	tested	1206:1211	arg1	preparations					1213:1224	tested preparations	1206:1224	tested preparations	1206:1224	Similar results were obtained for poloxamer 407, whereas it decreased the surface tension of tested preparations.
29736498	4	76	dep	kinematic	764:772	arg1	couple					774:779	couple	774:779	couple	774:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	0	77	theme	Tribological	65:76	arg1	Properties					78:87	- Physicochemical and Tribological Properties	43:87	- Physicochemical and Tribological Properties	43:87	Artificial Mucin-based Saliva Preparations - Physicochemical and Tribological Properties.
29736498	4	78	theme	kinematic	764:772	arg1	Ti-6Al-7Nb					753:762	titanium alloy Ti-6Al-7Nb	738:762	titanium alloy Ti-6Al-7Nb kinematic couple	738:779	A wide range of properties were characterised: physicochemical (changes of pH, conductivity and surface tension over time: 1, 24, 96, 168 h after mixing ingredients), rheological (viscosity and viscoelasticity), tribological (coefficient of friction for titanium alloy Ti-6Al-7Nb kinematic couple) and corrosive (open circuit potential, corrosion potential, polarisation resistance).
29736498	1	79	theme	similar	182:188	arg1	properties					171:180	properties	171:180	properties similar to natural saliva	171:206	PURPOSE To create and investigate artificial mucin-based saliva substitutes with properties similar to natural saliva.
30976088	6	0	theme	host	1321:1324	arg1	interactions					1340:1351	the host protein-virus interactions	1317:1351	the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission	1317:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	6	1	theme	adaptive	1229:1236	arg1	mechanisms					1245:1254	innate and adaptive immune mechanisms	1218:1254	innate and adaptive immune mechanisms of broad HIV neutralization	1218:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	5	2	theme	glycan-dependent	1110:1125	arg1	antibodies					1139:1148	glycan-dependent V3-specific antibodies	1110:1148	glycan-dependent V3-specific antibodies such as PGT128	1110:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	2	theme	glycan-dependent	1110:1125	arg1	PGT128					1158:1163	PGT128	1158:1163	PGT128	1158:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	6	3	theme	protein-virus	1326:1338	arg1	interactions					1340:1351	the host protein-virus interactions	1317:1351	the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission	1317:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	6	4	theme	innate	1218:1223	arg1	mechanisms					1245:1254	innate and adaptive immune mechanisms	1218:1254	innate and adaptive immune mechanisms of broad HIV neutralization	1218:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	5	5	theme	V3-specific	1127:1137	arg1	antibodies					1139:1148	glycan-dependent V3-specific antibodies	1110:1148	glycan-dependent V3-specific antibodies such as PGT128	1110:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	5	theme	V3-specific	1127:1137	arg1	PGT128					1158:1163	PGT128	1158:1163	PGT128	1158:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	6	theme	V3	947:948	arg1	residues					950:957	Env V3 residues 321/322 and 326/327	943:977	Env V3 residues 321/322 and 326/327	943:977	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	2	7	with	interaction	491:501	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	6	8	theme	transmission	1411:1422	arg1	inefficiency					1381:1392	the natural inefficiency	1369:1392	the natural inefficiency of mucosal HIV-1 transmission	1369:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	1	9	theme	vaccine	267:273	arg1	development					252:262	the development	248:262	the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission	248:335	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	10	theme	Env	528:530	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	1	11	from	virions	150:156	arg1	factors					112:118	innate antiviral factors	95:118	innate antiviral factors at mucosal surfaces and HIV-1 virions	95:156	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	6	12	theme	further	1296:1302	arg1	insight					1304:1310	further insight	1296:1310	further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission	1296:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	4	13	theme	TNC	680:682	arg1	binding					684:690	TNC binding	680:690	TNC binding	680:690	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	4	14	theme	fn	798:799	arg1	domains					802:808	fibronectin-type III (fn) domains	776:808	fibronectin-type III (fn) domains	776:808	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	4	15	theme	TNC	740:742	arg1	fbg					767:769	fbg	767:769	fbg	767:769	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	4	15	theme	TNC	740:742	arg1	globe					760:764	the TNC fibrinogen-like globe	736:764	the TNC fibrinogen-like globe (fbg)	736:770	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	1	16	theme	natural	176:182	arg1	inefficiency					184:195	the natural inefficiency	172:195	the natural inefficiency of HIV-1 transmission	172:217	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	17	theme	Envelope	518:525	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	18	gly	glycoprotein	398:409	arg1	Tenascin-C					338:347	Tenascin-C	338:347	Tenascin-C (TNC)	338:353	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	18	gly	glycoprotein	398:409	arg1	glycoprotein					398:409	a large, hexameric extracellular matrix glycoprotein	358:409	glycoprotein	398:409	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	19	theme	matrix	391:396	arg1	Tenascin-C					338:347	Tenascin-C	338:347	Tenascin-C (TNC)	338:353	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	19	theme	matrix	391:396	arg1	glycoprotein					398:409	a large, hexameric extracellular matrix glycoprotein	358:409	glycoprotein	398:409	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	6	20	theme	HIV-1	1405:1409	arg1	transmission					1411:1422	mucosal HIV-1 transmission	1397:1422	mucosal HIV-1 transmission	1397:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	5	21	theme	neutralizing	1085:1096	arg1	activity					1098:1105	the broadly neutralizing activity	1073:1105	the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128	1073:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	1	22	theme	innate	95:100	arg1	factors					112:118	innate antiviral factors	95:118	innate antiviral factors at mucosal surfaces and HIV-1 virions	95:156	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	23	theme	extracellular	377:389	arg1	Tenascin-C					338:347	Tenascin-C	338:347	Tenascin-C (TNC)	338:353	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	23	theme	extracellular	377:389	arg1	glycoprotein					398:409	a large, hexameric extracellular matrix glycoprotein	358:409	glycoprotein	398:409	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	6	24	theme	mucosal	1397:1403	arg1	transmission					1411:1422	mucosal HIV-1 transmission	1397:1422	mucosal HIV-1 transmission	1397:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	1	25	theme	nonvaccine	279:288	arg1	strategies					290:299	nonvaccine strategies	279:299	nonvaccine strategies	279:299	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	1	26	theme	antiviral	102:110	arg1	factors					112:118	innate antiviral factors	95:118	innate antiviral factors at mucosal surfaces and HIV-1 virions	95:156	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	1	27	theme	HIV-1	200:204	arg1	transmission					206:217	HIV-1 transmission	200:217	HIV-1 transmission	200:217	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	28	theme	genital	441:447	arg1	fluids					449:454	genital fluids	441:454	genital fluids	441:454	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	1	29	theme	strategies	290:299	arg1	development					252:262	the development	248:262	the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission	248:335	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	30	theme	V3	545:546	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	1	31	from	surfaces	131:138	arg1	factors					112:118	innate antiviral factors	95:118	innate antiviral factors at mucosal surfaces and HIV-1 virions	95:156	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	5	32	theme	TNC-mediated	894:905	arg1	neutralization					907:920	TNC-mediated neutralization	894:920	TNC-mediated neutralization	894:920	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	1	33	theme	transmission	206:217	arg1	inefficiency					184:195	the natural inefficiency	172:195	the natural inefficiency of HIV-1 transmission	172:217	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	0	34	theme	Tenascin-C	16:25	arg1	envelope					37:44	HIV-1 envelope	31:44	HIV-1 envelope	31:44	Determinants of Tenascin-C and HIV-1 envelope binding and neutralization.
30976088	0	34	theme	Tenascin-C	16:25	arg1	Determinants					0:11	Determinants	0:11	Determinants of Tenascin-C	0:25	Determinants of Tenascin-C and HIV-1 envelope binding and neutralization.
30976088	6	35	theme	neutralization	1269:1282	arg1	mechanisms					1245:1254	innate and adaptive immune mechanisms	1218:1254	innate and adaptive immune mechanisms of broad HIV neutralization	1218:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	3	36	theme	interaction	620:630	arg1	determinants					600:611	the specific determinants	587:611	the specific determinants of the interaction between TNC and the HIV-1 Env	587:660	In this report, we characterize the specific determinants of the interaction between TNC and the HIV-1 Env.
30976088	4	37	theme	HIV-1	714:718	arg1	neutralization					696:709	neutralization	696:709	neutralization	696:709	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	4	37	theme	HIV-1	714:718	arg1	binding					684:690	TNC binding	680:690	TNC binding	680:690	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	2	38	theme	breast	425:430	arg1	milk					432:435	breast milk	425:435	breast milk	425:435	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	6	39	theme	natural	1373:1379	arg1	inefficiency					1381:1392	the natural inefficiency	1369:1392	the natural inefficiency of mucosal HIV-1 transmission	1369:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	6	40	theme	HIV	1265:1267	arg1	neutralization					1269:1282	broad HIV neutralization	1259:1282	broad HIV neutralization	1259:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	2	41	theme	hexameric	367:375	arg1	Tenascin-C					338:347	Tenascin-C	338:347	Tenascin-C (TNC)	338:353	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	41	theme	hexameric	367:375	arg1	glycoprotein					398:409	a large, hexameric extracellular matrix glycoprotein	358:409	glycoprotein	398:409	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	0	42	theme	HIV-1	31:35	arg1	envelope					37:44	HIV-1 envelope	31:44	HIV-1 envelope	31:44	Determinants of Tenascin-C and HIV-1 envelope binding and neutralization.
30976088	1	43	theme	mucosal	310:316	arg1	transmission					324:335	mucosal HIV-1 transmission	310:335	mucosal HIV-1 transmission	310:335	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	44	theme	variable	533:540	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	5	45	theme	V3	1018:1019	arg1	loop					1021:1024	the V3 loop	1014:1024	the V3 loop	1014:1024	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	0	46	dep	binding	46:52	arg1	envelope					37:44	HIV-1 envelope	31:44	HIV-1 envelope	31:44	Determinants of Tenascin-C and HIV-1 envelope binding and neutralization.
30976088	0	46	dep	binding	46:52	arg1	Determinants					0:11	Determinants	0:11	Determinants of Tenascin-C	0:25	Determinants of Tenascin-C and HIV-1 envelope binding and neutralization.
30976088	1	47	theme	HIV-1	318:322	arg1	transmission					324:335	mucosal HIV-1 transmission	310:335	mucosal HIV-1 transmission	310:335	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	4	48	theme	newly-mapped	836:847	arg1	structure					856:864	its newly-mapped glycan structure	832:864	its newly-mapped glycan structure	832:864	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	5	49	theme	loop	1021:1024	arg1	motif					1005:1009	the IGDIR motif	995:1009	the IGDIR motif of the V3 loop	995:1024	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	6	50	theme	striking	1192:1199	arg1	parallel					1201:1208	a striking parallel	1190:1208	a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization	1190:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	1	51	theme	mucosal	123:129	arg1	surfaces					131:138	mucosal surfaces	123:138	mucosal surfaces	123:138	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	6	52	theme	broad	1259:1263	arg1	neutralization					1269:1282	broad HIV neutralization	1259:1282	broad HIV neutralization	1259:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	4	53	theme	fibronectin-type	776:791	arg1	domains					802:808	fibronectin-type III (fn) domains	776:808	fibronectin-type III (fn) domains	776:808	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	6	54	theme	immune	1238:1243	arg1	mechanisms					1245:1254	innate and adaptive immune mechanisms	1218:1254	innate and adaptive immune mechanisms of broad HIV neutralization	1218:1282	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	3	55	theme	HIV-1	652:656	arg1	Env					658:660	the HIV-1 Env	648:660	the HIV-1 Env	648:660	In this report, we characterize the specific determinants of the interaction between TNC and the HIV-1 Env.
30976088	3	56	theme	specific	591:598	arg1	determinants					600:611	the specific determinants	587:611	the specific determinants of the interaction between TNC and the HIV-1 Env	587:660	In this report, we characterize the specific determinants of the interaction between TNC and the HIV-1 Env.
30976088	4	57	theme	glycan	849:854	arg1	structure					856:864	its newly-mapped glycan structure	832:864	its newly-mapped glycan structure	832:864	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	5	58	theme	IGDIR	999:1003	arg1	motif					1005:1009	the IGDIR motif	995:1009	the IGDIR motif of the V3 loop	995:1024	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	4	59	theme	fibrinogen-like	744:758	arg1	fbg					767:769	fbg	767:769	fbg	767:769	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	4	59	theme	fibrinogen-like	744:758	arg1	globe					760:764	the TNC fibrinogen-like globe	736:764	the TNC fibrinogen-like globe (fbg)	736:770	We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure.
30976088	1	60	theme	HIV-1	144:148	arg1	virions					150:156	HIV-1 virions	144:156	HIV-1 virions	144:156	Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission.
30976088	2	61	theme	HIV-1	512:516	arg1	loop					549:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	the HIV-1 Envelope (Env) variable 3 (V3) loop	508:552	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	5	62	dep	residues	950:957	arg1	326/327					971:977	326/327	971:977	326/327	971:977	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	62	dep	residues	950:957	arg1	321/322					959:965	321/322	959:965	321/322	959:965	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	6	63	theme	responsible	1353:1363	arg1	interactions					1340:1351	the host protein-virus interactions	1317:1351	the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission	1317:1422	Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.
30976088	5	64	theme	Env	943:945	arg1	residues					950:957	Env V3 residues 321/322 and 326/327	943:977	Env V3 residues 321/322 and 326/327	943:977	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	65	theme	N332	1039:1042	arg1	glycan					1044:1049	the N332 glycan	1035:1049	the N332 glycan	1035:1049	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	5	66	theme	antibodies	1139:1148	arg1	activity					1098:1105	the broadly neutralizing activity	1073:1105	the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128	1073:1163	Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128.
30976088	2	67	theme	large	360:364	arg1	Tenascin-C					338:347	Tenascin-C	338:347	Tenascin-C (TNC)	338:353	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
30976088	2	67	theme	large	360:364	arg1	glycoprotein					398:409	a large, hexameric extracellular matrix glycoprotein	358:409	glycoprotein	398:409	Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop.
29968542	10	0	theme	accellular	1618:1627	arg1	bioactivity					1629:1639	accellular bioactivity	1618:1639	accellular bioactivity in SBF, loss weight and wash out behavior	1618:1681	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	0	theme	accellular	1618:1627	arg1	capacity					1577:1584	capacity	1577:1584	capacity	1577:1584	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	6	1	theme	cells	887:891	arg1	behavior					852:859	The behavior	848:859	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes	848:929	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	11	2	theme	bioactive	1819:1827	arg1	nanoparticles					1835:1847	bioactive glass nanoparticles	1819:1847	bioactive glass nanoparticles	1819:1847	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	10	3	theme	sodium	1483:1488	arg1	%					1500:1500	sodium alginate 1%	1483:1500	sodium alginate 1%	1483:1500	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	5	4	theme	CaO/P2O5	653:660	arg1	effect					643:648	The effect	639:648	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes	639:827	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	6	5	theme	mesenchymal	870:880	arg1	hMSCs					894:898	hMSCs	894:898	hMSCs	894:898	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	6	5	theme	mesenchymal	870:880	arg1	cells					887:891	human mesenchymal stem cells	864:891	human mesenchymal stem cells (hMSCs)	864:899	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	11	6	from	engineering	1882:1892	arg1	pastes					1769:1774	the pastes	1765:1774	the pastes prepared with sodium alginate solution and bioactive glass nanoparticles	1765:1847	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	11	6	from	engineering	1882:1892	arg1	beneficial					1856:1865	beneficial	1856:1865	beneficial	1856:1865	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	1	7	theme	Bioactive	160:168	arg1	glasses					170:176	BACKGROUND Bioactive glasses	149:176	BACKGROUND Bioactive glasses with different compositions	149:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	1	7	theme	Bioactive	160:168	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	8	8	theme	storage	1138:1144	arg1	modulus					1146:1152	The storage modulus	1134:1152	The storage modulus of all pastes	1134:1166	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	8	8	theme	storage	1138:1144	arg1	larger					1172:1177	larger	1172:1177	larger	1172:1177	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	10	9	contain	carrying	1568:1575	arg2	characteristics					1601:1615	injectability characteristics	1587:1615	injectability characteristics	1587:1615	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	9	contain	carrying	1568:1575	arg2	capacity					1577:1584	capacity	1577:1584	capacity	1577:1584	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	9	contain	carrying	1568:1575	arg2	bioactivity					1629:1639	accellular bioactivity	1618:1639	accellular bioactivity in SBF, loss weight and wash out behavior	1618:1681	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	9	contain	carrying	1568:1575	arg2	proliferation					1684:1696	proliferation	1684:1696	proliferation	1684:1696	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	9	contain	carrying	1568:1575	arg2	differentiation					1702:1716	differentiation	1702:1716	differentiation	1702:1716	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	9	contain	carrying	1568:1575	arg1	mineral					1560:1566	mineral	1560:1566	mineral	1560:1566	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	2	10	theme	pastes	319:324	arg1	purposes					384:391	the purposes	380:391	the purposes of this study	380:405	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	10	theme	pastes	319:324	arg1	characterization					290:305	characterization	290:305	characterization	290:305	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	10	theme	pastes	319:324	arg1	development					274:284	development	274:284	development	274:284	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	10	theme	pastes	319:324	arg1	Preparation					261:271	Preparation	261:271	Preparation	261:271	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	11	11	from	beneficial	1856:1865	arg1	engineering					1882:1892	bone tissue engineering	1870:1892	bone tissue engineering	1870:1892	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	5	12	theme	P/L	684:686	arg1	ratios					689:694	liquid (P/L) ratios	676:694	liquid (P/L) ratios	676:694	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	3	13	theme	alginate	570:577	arg1	solutions					579:587	sodium alginate solutions	563:587	sodium alginate solutions	563:587	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	5	14	theme	pastes	822:827	arg1	biodegradation					741:754	biodegradation	741:754	biodegradation	741:754	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	14	theme	pastes	822:827	arg1	bioactivity					781:791	bioactivity	781:791	bioactivity	781:791	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	14	theme	pastes	822:827	arg1	behavior					806:813	cellular behavior	797:813	cellular behavior	797:813	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	14	theme	pastes	822:827	arg1	properties					769:778	rheological properties	757:778	rheological properties	757:778	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	14	theme	pastes	822:827	arg1	injectability					726:738	injectability	726:738	injectability	726:738	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	8	15	theme	pastes	1161:1166	arg1	modulus					1146:1152	The storage modulus	1134:1152	The storage modulus of all pastes	1134:1166	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	8	15	theme	pastes	1161:1166	arg1	larger					1172:1177	larger	1172:1177	larger	1172:1177	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	11	16	theme	alginate	1797:1804	arg1	solution					1806:1813	sodium alginate solution	1790:1813	sodium alginate solution	1790:1813	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	10	17	theme	0.8	1520:1522	arg1	ratio					1511:1515	P/L ratio	1507:1515	P/L ratio of 0.8	1507:1522	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	0	18	theme	Alginate	110:117	arg1	Concentration					119:131	Sodium Alginate Concentration	103:131	Sodium Alginate Concentration	103:131	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	11	19	theme	bone	1870:1873	arg1	engineering					1882:1892	bone tissue engineering	1870:1892	bone tissue engineering	1870:1892	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	8	20	from	modulus	1193:1199	arg1	range					1218:1222	the frequency range	1204:1222	the frequency range of 0.1-100 s-1	1204:1237	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	7	21	dep	RESULTS	1055:1061	arg1	exhibited					1101:1109	exhibited	1101:1109	exhibited a thixotropy behavior	1101:1131	RESULTS By adding sodium alginate, the pastes exhibited a thixotropy behavior.
29968542	5	22	theme	powder	666:671	arg1	effect					643:648	The effect	639:648	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes	639:827	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	23	theme	liquid	676:681	arg1	ratios					689:694	liquid (P/L) ratios	676:694	liquid (P/L) ratios	676:694	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	0	24	theme	Bioactive	50:58	arg1	Paste					76:80	Bioactive Glass Composite Paste	50:80	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	7	25	theme	thixotropy	1113:1122	arg1	behavior					1124:1131	a thixotropy behavior	1111:1131	a thixotropy behavior	1111:1131	RESULTS By adding sodium alginate, the pastes exhibited a thixotropy behavior.
29968542	6	26	theme	biomineralization	958:974	arg1	assay					976:980	biomineralization assay	958:980	biomineralization assay	958:980	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	0	27	theme	Ratios	141:146	arg1	Effect					83:88	Effect	83:88	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	0	28	theme	Composite	66:74	arg1	Paste					76:80	Bioactive Glass Composite Paste	50:80	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	6	29	dep	assay	951:955	arg1	tests					1048:1052	tests	1048:1052	tests	1048:1052	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	5	30	theme	alginate	700:707	arg1	concentration					709:721	alginate concentration	700:721	alginate concentration	700:721	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	9	31	theme	Cytotoxicity	1240:1251	arg1	results					1264:1270	Cytotoxicity evaluation results	1240:1270	Cytotoxicity evaluation results	1240:1270	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	3	32	dep	OBJECTIVE	408:416	arg1	produced					450:457	produced	450:457	were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	445:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	11	33	dep	CONCLUSION	1728:1737	arg1	results					1756:1762	the results	1752:1762	the results	1752:1762	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	0	34	theme	Sodium	32:37	arg1	Alginate					39:46	Sodium Alginate	32:46	Sodium Alginate	32:46	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	6	35	theme	Alizarin	1026:1033	arg1	staining					1039:1046	Alizarin red staining	1026:1046	Alizarin red staining	1026:1046	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	11	36	theme	sodium	1790:1795	arg1	solution					1806:1813	sodium alginate solution	1790:1813	sodium alginate solution	1790:1813	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	5	37	theme	cellular	797:804	arg1	behavior					806:813	cellular behavior	797:813	cellular behavior	797:813	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	3	38	theme	bioactive	480:488	arg1	nanoparticles					496:508	sol-gel derived bioactive glass nanoparticles	464:508	sol-gel derived bioactive glass nanoparticles	464:508	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	0	39	theme	hMSCs	14:18	arg1	Response					20:27	hMSCs Response	14:27	hMSCs Response to Sodium Alginate	14:46	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	10	40	theme	loss	1649:1652	arg1	weight					1654:1659	loss weight	1649:1659	loss weight	1649:1659	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	6	41	theme	Acridine	997:1004	arg1	staining					1013:1020	Acridine orange staining	997:1020	Acridine orange staining	997:1020	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	3	42	theme	injectable	422:431	arg1	pastes					438:443	The injectable bone pastes	418:443	The injectable bone pastes	418:443	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	10	43	theme	mineral	1560:1566	arg1	terms					1551:1555	terms	1551:1555	terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs	1551:1725	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	3	44	theme	sol-gel	464:470	arg1	nanoparticles					496:508	sol-gel derived bioactive glass nanoparticles	464:508	sol-gel derived bioactive glass nanoparticles	464:508	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	9	45	theme	critical	1298:1305	arg1	amount					1307:1312	a critical amount	1296:1312	a critical amount of bioactive glass	1296:1331	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	9	45	theme	critical	1298:1305	arg1	glass					1327:1331	bioactive glass	1317:1331	bioactive glass	1317:1331	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	10	46	from	behavior	1539:1546	arg1	terms					1551:1555	terms	1551:1555	terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs	1551:1725	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	47	theme	glass	1439:1443	arg1	nanoparticles					1445:1457	bioactive glass nanoparticles	1429:1457	bioactive glass nanoparticles	1429:1457	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	3	48	theme	various	515:521	arg1	ratios					532:537	various CaO/P2O5 ratios	515:537	various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	515:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	10	49	theme	hMSCs	1721:1725	arg1	differentiation					1702:1716	differentiation	1702:1716	differentiation	1702:1716	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	49	theme	hMSCs	1721:1725	arg1	proliferation					1684:1696	proliferation	1684:1696	proliferation	1684:1696	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	49	theme	hMSCs	1721:1725	arg1	capacity					1577:1584	capacity	1577:1584	capacity	1577:1584	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	50	theme	injectability	1587:1599	arg1	characteristics					1601:1615	injectability characteristics	1587:1615	injectability characteristics	1587:1615	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	50	theme	injectability	1587:1599	arg1	capacity					1577:1584	capacity	1577:1584	capacity	1577:1584	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	9	51	theme	glass	1327:1331	arg1	amount					1307:1312	a critical amount	1296:1312	a critical amount of bioactive glass	1296:1331	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	9	51	theme	glass	1327:1331	arg1	glass					1327:1331	bioactive glass	1317:1331	bioactive glass	1317:1331	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	1	52	used	used	228:231	arg2	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	1	52	used	used	228:231	arg2	glasses					170:176	BACKGROUND Bioactive glasses	149:176	BACKGROUND Bioactive glasses with different compositions	149:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	11	53	theme	glass	1829:1833	arg1	nanoparticles					1835:1847	bioactive glass nanoparticles	1819:1847	bioactive glass nanoparticles	1819:1847	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	3	54	theme	19	542:543	arg1	ratios					532:537	various CaO/P2O5 ratios	515:537	various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	515:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	0	55	dep	Evaluation	0:9	arg1	Effect					83:88	Effect	83:88	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	0	55	dep	Evaluation	0:9	arg1	Paste					76:80	Bioactive Glass Composite Paste	50:80	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	7	56	theme	sodium	1073:1078	arg1	alginate					1080:1087	sodium alginate	1073:1087	sodium alginate	1073:1087	RESULTS By adding sodium alginate, the pastes exhibited a thixotropy behavior.
29968542	1	57	theme	tissue	241:246	arg1	glasses					170:176	BACKGROUND Bioactive glasses	149:176	BACKGROUND Bioactive glasses with different compositions	149:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	1	57	theme	tissue	241:246	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	3	58	link	derived	472:478	arg1	nanoparticles					496:508	sol-gel derived bioactive glass nanoparticles	464:508	sol-gel derived bioactive glass nanoparticles	464:508	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	10	59	theme	alginate	1490:1497	arg1	%					1500:1500	sodium alginate 1%	1483:1500	sodium alginate 1%	1483:1500	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	6	60	theme	stem	882:885	arg1	hMSCs					894:898	hMSCs	894:898	hMSCs	894:898	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	6	60	theme	stem	882:885	arg1	cells					887:891	human mesenchymal stem cells	864:891	human mesenchymal stem cells (hMSCs)	864:899	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	2	61	theme	repair	355:360	arg1	applications					362:373	bone repair applications	350:373	bone repair applications	350:373	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	3	62	theme	9.5	546:548	arg1	ratios					532:537	various CaO/P2O5 ratios	515:537	various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	515:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	1	63	theme	BACKGROUND	149:158	arg1	glasses					170:176	BACKGROUND Bioactive glasses	149:176	BACKGROUND Bioactive glasses with different compositions	149:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	1	63	theme	BACKGROUND	149:158	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	6	64	theme	human	864:868	arg1	hMSCs					894:898	hMSCs	894:898	hMSCs	894:898	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	6	64	theme	human	864:868	arg1	cells					887:891	human mesenchymal stem cells	864:891	human mesenchymal stem cells (hMSCs)	864:899	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	10	65	from	bioactivity	1629:1639	arg1	weight					1654:1659	loss weight	1649:1659	loss weight	1649:1659	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	65	from	bioactivity	1629:1639	arg1	wash					1665:1668	wash	1665:1668	wash	1665:1668	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	65	from	bioactivity	1629:1639	arg1	SBF					1644:1646	SBF	1644:1646	SBF	1644:1646	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	10	66	theme	P/L	1507:1509	arg1	ratio					1511:1515	P/L ratio	1507:1515	P/L ratio of 0.8	1507:1522	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	3	67	theme	solutions	579:587	arg1	ratios					532:537	various CaO/P2O5 ratios	515:537	various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	515:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	8	68	theme	loss	1188:1191	arg1	modulus					1193:1199	the loss modulus	1184:1199	the loss modulus in the frequency range of 0.1-100 s-1	1184:1237	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	6	69	from	behavior	852:859	arg1	presence					908:915	the presence	904:915	the presence of the pastes	904:929	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	0	70	theme	Sodium	103:108	arg1	Concentration					119:131	Sodium Alginate Concentration	103:131	Sodium Alginate Concentration	103:131	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	2	71	theme	alginate	310:317	arg1	pastes					319:324	alginate pastes	310:324	alginate pastes containing bioglass	310:344	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	9	72	theme	hMSCs	1387:1391	arg1	viability					1374:1382	the viability	1370:1382	the viability of hMSCs	1370:1391	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	6	73	theme	pastes	924:929	arg1	presence					908:915	the presence	904:915	the presence of the pastes	904:929	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	2	74	theme	bone	350:353	arg1	applications					362:373	bone repair applications	350:373	bone repair applications	350:373	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	0	75	theme	Concentration	119:131	arg1	Effect					83:88	Effect	83:88	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	5	76	from	effect	643:648	arg1	behavior					806:813	cellular behavior	797:813	cellular behavior	797:813	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	76	from	effect	643:648	arg1	bioactivity					781:791	bioactivity	781:791	bioactivity	781:791	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	76	from	effect	643:648	arg1	biodegradation					741:754	biodegradation	741:754	biodegradation	741:754	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	76	from	effect	643:648	arg1	properties					769:778	rheological properties	757:778	rheological properties	757:778	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	5	76	from	effect	643:648	arg1	injectability					726:738	injectability	726:738	injectability	726:738	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	0	77	theme	P/L	137:139	arg1	Ratios					141:146	P/L Ratios	137:146	P/L Ratios	137:146	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	3	78	theme	different	594:602	arg1	concentrations					604:617	different concentrations	594:617	different concentrations of 1, 2 and 4 wt	594:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	3	79	theme	sodium	563:568	arg1	solutions					579:587	sodium alginate solutions	563:587	sodium alginate solutions	563:587	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	6	80	theme	ALP	983:985	arg1	activity					987:994	ALP activity	983:994	ALP activity	983:994	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	8	81	theme	s-1	1235:1237	arg1	range					1218:1222	the frequency range	1204:1222	the frequency range of 0.1-100 s-1	1204:1237	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	0	82	theme	Glass	60:64	arg1	Paste					76:80	Bioactive Glass Composite Paste	50:80	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	11	83	theme	tissue	1875:1880	arg1	engineering					1882:1892	bone tissue engineering	1870:1892	bone tissue engineering	1870:1892	CONCLUSION According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
29968542	1	84	theme	different	183:191	arg1	compositions					193:204	different compositions	183:204	different compositions	183:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	6	85	theme	MTT	947:949	arg1	assay					951:955	MTT assay	947:955	MTT assay	947:955	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	0	86	theme	CaO/P2O5	93:100	arg1	Effect					83:88	Effect	83:88	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	9	87	theme	evaluation	1253:1262	arg1	results					1264:1270	Cytotoxicity evaluation results	1240:1270	Cytotoxicity evaluation results	1240:1270	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	6	88	theme	red	1035:1037	arg1	staining					1039:1046	Alizarin red staining	1026:1046	Alizarin red staining	1026:1046	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	2	89	theme	study	401:405	arg1	characterization					290:305	characterization	290:305	characterization	290:305	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	89	theme	study	401:405	arg1	Preparation					261:271	Preparation	261:271	Preparation	261:271	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	89	theme	study	401:405	arg1	development					274:284	development	274:284	development	274:284	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	89	theme	study	401:405	arg1	purposes					384:391	the purposes	380:391	the purposes of this study	380:405	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	10	90	theme	optimum	1531:1537	arg1	behavior					1539:1546	optimum behavior	1531:1546	optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs	1531:1725	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	0	91	theme	Response	20:27	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.	0:147	Evaluation of hMSCs Response to Sodium Alginate / Bioactive Glass Composite Paste: Effect of CaO/P2O5, Sodium Alginate Concentration and P/L Ratios.
29968542	6	92	theme	orange	1006:1011	arg1	staining					1013:1020	Acridine orange staining	997:1020	Acridine orange staining	997:1020	The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
29968542	3	93	theme	glass	490:494	arg1	nanoparticles					496:508	sol-gel derived bioactive glass nanoparticles	464:508	sol-gel derived bioactive glass nanoparticles	464:508	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	5	94	theme	rheological	757:767	arg1	properties					769:778	rheological properties	757:778	rheological properties	757:778	The effect of CaO/P2O5 and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied.
29968542	2	95	contain	containing	326:335	arg2	bioglass					337:344	bioglass	337:344	bioglass	337:344	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	2	95	contain	containing	326:335	arg1	pastes					319:324	alginate pastes	310:324	alginate pastes containing bioglass	310:344	Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
29968542	3	96	theme	bone	433:436	arg1	pastes					438:443	The injectable bone pastes	418:443	The injectable bone pastes	418:443	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	3	97	theme	wt	633:634	arg1	concentrations					604:617	different concentrations	594:617	different concentrations of 1, 2 and 4 wt	594:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	3	98	theme	derived	472:478	arg1	nanoparticles					496:508	sol-gel derived bioactive glass nanoparticles	464:508	sol-gel derived bioactive glass nanoparticles	464:508	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	1	99	with	glasses	170:176	arg1	compositions					193:204	different compositions	183:204	different compositions	183:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	10	100	theme	bioactive	1429:1437	arg1	nanoparticles					1445:1457	bioactive glass nanoparticles	1429:1457	bioactive glass nanoparticles	1429:1457	The pastes made of bioactive glass nanoparticles with CaO/P2O5 = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
29968542	3	101	theme	CaO/P2O5	523:530	arg1	ratios					532:537	various CaO/P2O5 ratios	515:537	various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt	515:634	OBJECTIVE The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/P2O5 ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.
29968542	9	102	theme	bioactive	1317:1325	arg1	glass					1327:1331	bioactive glass	1317:1331	bioactive glass	1317:1331	Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk.
29968542	8	103	theme	frequency	1208:1216	arg1	range					1218:1222	the frequency range	1204:1222	the frequency range of 0.1-100 s-1	1204:1237	The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1.
29968542	1	104	theme	bone	236:239	arg1	glasses					170:176	BACKGROUND Bioactive glasses	149:176	BACKGROUND Bioactive glasses with different compositions	149:204	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
29968542	1	104	theme	bone	236:239	arg1	engineering					248:258	bone tissue engineering	236:258	bone tissue engineering	236:258	BACKGROUND Bioactive glasses with different compositions have been extensively used as bone tissue engineering.
30024582	4	0	theme	loading	989:995	arg1	efficiencies					997:1008	loading efficiencies	989:1008	loading efficiencies up to 100%	989:1019	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	3	1	theme	sulfate	539:545	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	1	theme	sulfate	539:545	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	6	2	theme	soluble	1369:1375	arg1	GAGs					1377:1380	soluble GAGs	1369:1380	soluble GAGs	1369:1380	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	13	3	link	crosslinked	2424:2434	arg1	hydrogels					2454:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	11	4	theme	lysozyme-loaded	2192:2206	arg1	microgels					2213:2221	lysozyme-loaded CSMA microgels	2192:2221	lysozyme-loaded CSMA microgels	2192:2221	Finally, lysozyme-loaded CSMA microgels were embedded into a thermosensitive hydrogel scaffold.
30024582	3	5	theme	chondroitin	527:537	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	5	theme	chondroitin	527:537	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	1	6	theme	cell-to-cell	272:283	arg1	processes					295:303	cell-to-cell signaling processes	272:303	cell-to-cell signaling processes	272:303	Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes.
30024582	10	7	theme	weaker	2147:2152	arg1	interactions					2169:2180	weaker protein-matrix interactions	2147:2180	weaker protein-matrix interactions	2147:2180	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	9	8	theme	diffusion	1764:1772	arg1	coefficients					1774:1785	Lysozyme diffusion coefficients	1755:1785	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels	1755:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	13	9	contain	have	2464:2467	arg2	potential					2469:2477	potential	2469:2477	potential as controlled release depots for cationic proteins	2469:2528	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	13	9	contain	have	2464:2467	arg1	hydrogels					2454:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	12	10	theme	lysozyme	2319:2326	arg1	release					2328:2334	complete lysozyme release	2310:2334	complete lysozyme release	2310:2334	These composite systems showed complete lysozyme release in ∼58 days as opposed to only 3 days for GAG-free scaffolds.
30024582	8	11	dep	disaccharide	1709:1720	arg1	2					1742:1742	2	1742:1742	2	1742:1742	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	8	11	dep	disaccharide	1709:1720	arg1	1					1724:1724	1	1724:1724	1	1724:1724	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	3	12	from	swelling	701:708	arg1	microgels					780:788	the microgels	776:788	the microgels dispersed in buffers (pH 7.4)	776:818	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	10	13	theme	negative	2065:2072	arg1	charges					2074:2080	the negative charges	2061:2080	the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions	2061:2180	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	11	14	theme	hydrogel	2260:2267	arg1	scaffold					2269:2276	a thermosensitive hydrogel scaffold	2242:2276	a thermosensitive hydrogel scaffold	2242:2276	Finally, lysozyme-loaded CSMA microgels were embedded into a thermosensitive hydrogel scaffold.
30024582	3	15	theme	lysozyme	721:728	arg1	distribution					730:741	intra-gel lysozyme distribution	711:741	intra-gel lysozyme distribution	711:741	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	16	theme	diameters	591:599	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	16	theme	diameters	591:599	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	6	17	theme	anionic	1413:1419	arg1	density					1428:1434	increased local anionic charge density	1397:1434	increased local anionic charge density in the GAG networks	1397:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	1	18	dep	factors	251:257	arg1	e.g.					239:242	e.g.	239:242	e.g.	239:242	Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes.
30024582	6	19	theme	Complex	1230:1236	arg1	coacervates					1238:1248	Complex coacervates	1230:1248	Complex coacervates inside GAG microgels	1230:1269	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	4	20	theme	mM	870:871	arg1	buffer					873:878	20 mM buffer	867:878	20 mM buffer	867:878	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	9	21	from	0.027	1790:1794	arg1	HAMA					1799:1802	HAMA	1799:1802	HAMA	1799:1802	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	9	22	theme	μm2	1815:1817	arg1	s-1					1819:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	3	23	theme	diffusion	751:759	arg1	coefficient					761:771	its diffusion coefficient	747:771	its diffusion coefficient	747:771	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	8	24	theme	buffer	1662:1667	arg1	strength					1646:1653	the ionic strength	1636:1653	the ionic strength of the buffer	1636:1667	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	3	25	theme	ionic	831:835	arg1	strengths					837:845	varying ionic strengths	823:845	varying ionic strengths	823:845	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	7	26	theme	microgel	1512:1519	arg1	networks					1521:1528	the negatively charged microgel networks	1489:1528	the negatively charged microgel networks	1489:1528	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	9	27	from	coefficients	1774:1785	arg1	microgels					1831:1839	CSMA microgels	1826:1839	CSMA microgels	1826:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	10	28	theme	similar	1991:1997	arg1	trends					1999:2004	similar trends	1991:2004	similar trends	1991:2004	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	3	29	used	used	619:622	arg2	methacrylate					503:514	Monodisperse hyaluronic acid methacrylate	474:514	Monodisperse hyaluronic acid methacrylate (HAMA)	474:521	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	29	used	used	619:622	arg2	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	29	used	used	619:622	arg2	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	29	used	used	619:622	arg2	HAMA					517:520	HAMA	517:520	HAMA	517:520	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	4	30	theme	mg-1	920:923	arg1	microspheres					929:940	3 and 4 mg mg-1 dry microspheres	909:940	3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively	909:981	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	7	31	dep	factor	1561:1566	arg1	up					1553:1554	up	1553:1554	up	1553:1554	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	0	32	theme	cationic	60:67	arg1	protein					69:75	a cationic protein	58:75	a cationic protein	58:75	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	10	33	theme	lysozyme	2016:2023	arg1	diffusion					2025:2033	lysozyme diffusion	2016:2033	lysozyme diffusion	2016:2033	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	5	34	theme	GAG-lysozyme	1154:1165	arg1	coacervates					1175:1185	GAG-lysozyme complex coacervates	1154:1185	GAG-lysozyme complex coacervates based on soluble GAGs found in literature	1154:1227	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	13	35	theme	engineering	2540:2550	arg1	applications					2552:2563	tissue engineering applications	2533:2563	tissue engineering applications	2533:2563	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	8	36	theme	anionic	1690:1696	arg1	groups					1698:1703	anionic groups	1690:1703	anionic groups	1690:1703	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	1	37	theme	biomedical	170:179	arg1	applications					181:192	biomedical applications	170:192	biomedical applications	170:192	Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes.
30024582	7	38	theme	cationic	1468:1475	arg1	lysozyme					1477:1484	cationic lysozyme	1468:1484	cationic lysozyme	1468:1484	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	9	39	theme	170	1855:1857	arg1	mM					1859:1860	mM	1859:1860	mM	1859:1860	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	0	40	theme	glycosaminoglycan	95:111	arg1	microgels					113:121	monodisperse glycosaminoglycan microgels	82:121	monodisperse glycosaminoglycan microgels	82:121	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	4	41	dep	efficiencies	997:1008	arg1	%					1019:1019	%	1019:1019	%	1019:1019	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	13	42	theme	cationic	2512:2519	arg1	proteins					2521:2528	cationic proteins	2512:2528	cationic proteins	2512:2528	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	5	43	theme	disaccharide 	1049:1061	arg1	stoichiometries					1030:1044	Binding stoichiometries	1022:1044	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.	1022:1228	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	13	44	theme	tissue	2533:2538	arg1	applications					2552:2563	tissue engineering applications	2533:2563	tissue engineering applications	2533:2563	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	0	45	theme	protein	69:75	arg1	loading					27:33	loading	27:33	loading	27:33	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	0	45	theme	protein	69:75	arg1	release					47:53	tunable release	39:53	tunable release of a cationic protein from monodisperse glycosaminoglycan microgels	39:121	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	3	46	from	absorption	637:646	arg1	microgels					780:788	the microgels	776:788	the microgels dispersed in buffers (pH 7.4)	776:818	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	2	47	theme	protein	389:395	arg1	kinetics					405:412	their protein release kinetics	383:412	their protein release kinetics upon encapsulation in hydrogel scaffolds	383:453	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	12	48	theme	GAG-free	2378:2385	arg1	scaffolds					2387:2395	GAG-free scaffolds	2378:2395	GAG-free scaffolds	2378:2395	These composite systems showed complete lysozyme release in ∼58 days as opposed to only 3 days for GAG-free scaffolds.
30024582	1	49	theme	signaling	285:293	arg1	processes					295:303	cell-to-cell signaling processes	272:303	cell-to-cell signaling processes	272:303	Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes.
30024582	4	50	dep	%	1019:1019	arg1	100					1016:1018	100	1016:1018	100	1016:1018	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	12	51	theme	complete	2310:2317	arg1	release					2328:2334	complete lysozyme release	2310:2334	complete lysozyme release	2310:2334	These composite systems showed complete lysozyme release in ∼58 days as opposed to only 3 days for GAG-free scaffolds.
30024582	9	52	theme	release	1882:1888	arg1	duration					1870:1877	duration	1870:1877	duration of release 14 and 28 days respectively	1870:1916	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	2	53	theme	hydrogel	436:443	arg1	scaffolds					445:453	hydrogel scaffolds	436:453	hydrogel scaffolds	436:453	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	13	54	theme	controlled	2482:2491	arg1	depots					2501:2506	controlled release depots	2482:2506	controlled release depots	2482:2506	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	7	55	theme	a	1559:1559	arg1	factor					1561:1566	a factor 2	1559:1568	a factor 2 in diameter	1559:1580	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	4	56	theme	HAMA	946:949	arg1	microgels					960:968	HAMA and CSMA microgels	946:968	HAMA and CSMA microgels respectively	946:981	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	10	57	theme	Photobleaching	1948:1961	arg1	measurements					1970:1981	Photobleaching (FRAP) measurements	1948:1981	Photobleaching (FRAP) measurements	1948:1981	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	3	58	theme	model	662:666	arg1	lysozyme					677:684	lysozyme	677:684	lysozyme	677:684	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	58	theme	model	662:666	arg1	protein					668:674	a cationic model protein	651:674	a cationic model protein (lysozyme)	651:685	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	59	theme	Monodisperse	474:485	arg1	HAMA					517:520	HAMA	517:520	HAMA	517:520	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	59	theme	Monodisperse	474:485	arg1	methacrylate					503:514	Monodisperse hyaluronic acid methacrylate	474:514	Monodisperse hyaluronic acid methacrylate (HAMA)	474:521	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	0	60	theme	tunable	39:45	arg1	release					47:53	tunable release	39:53	tunable release of a cationic protein from monodisperse glycosaminoglycan microgels	39:121	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	13	61	theme	methacrylated	2436:2448	arg1	hydrogels					2454:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	4	62	theme	CSMA	955:958	arg1	microgels					960:968	HAMA and CSMA microgels	946:968	HAMA and CSMA microgels respectively	946:981	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	2	63	theme	microgels	348:356	arg1	potential					325:333	the potential	321:333	the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds	321:453	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	6	64	theme	ionic	1309:1313	arg1	strengths					1315:1323	higher ionic strengths	1302:1323	higher ionic strengths	1302:1323	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	9	65	located	found	1846:1850	arg1	buffer					1862:1867	170 mM buffer	1855:1867	170 mM buffer (duration of release 14 and 28 days respectively)	1855:1917	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	9	65	located	found	1846:1850	arg2	coefficients					1774:1785	Lysozyme diffusion coefficients	1755:1785	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels	1755:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	9	66	dep	buffer	1862:1867	arg1	duration					1870:1877	duration	1870:1877	duration of release 14 and 28 days respectively	1870:1916	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	2	67	from	encapsulation	419:431	arg1	scaffolds					445:453	hydrogel scaffolds	436:453	hydrogel scaffolds	436:453	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	3	68	theme	methacrylate	547:558	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	68	theme	methacrylate	547:558	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	1	69	theme	growth	244:249	arg1	factors					251:257	growth factors	244:257	growth factors	244:257	Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes.
30024582	13	70	theme	GAG	2450:2452	arg1	hydrogels					2454:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	10	71	theme	Fluorescence	1920:1931	arg1	Recovery					1933:1940	Fluorescence Recovery	1920:1940	Fluorescence Recovery After Photobleaching (FRAP) measurements	1920:1981	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	3	72	theme	de	698:699	arg1	swelling					701:708	microgel (de)swelling	688:708	microgel (de)swelling	688:708	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	2	73	theme	protein	362:368	arg1	delivery					370:377	protein delivery	362:377	protein delivery	362:377	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	3	74	theme	microgel	688:695	arg1	swelling					701:708	microgel (de)swelling	688:708	microgel (de)swelling	688:708	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	75	theme	micro-hydrogel	567:580	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	75	theme	micro-hydrogel	567:580	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	8	76	theme	Lysozyme	1583:1590	arg1	release					1592:1598	Lysozyme release	1583:1598	Lysozyme release from the microgels	1583:1617	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	9	77	theme	Lysozyme	1755:1762	arg1	coefficients					1774:1785	Lysozyme diffusion coefficients	1755:1785	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels	1755:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	3	78	theme	intra-gel	711:719	arg1	distribution					730:741	intra-gel lysozyme distribution	711:741	intra-gel lysozyme distribution	711:741	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	79	theme	CSMA	561:564	arg1	spheres					582:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres	527:588	chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm)	527:611	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	79	theme	CSMA	561:564	arg1	μm					609:610	diameters 500-700 μm	591:610	diameters 500-700 μm	591:610	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	6	80	theme	GAG	1443:1445	arg1	networks					1447:1454	the GAG networks	1439:1454	the GAG networks	1439:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	9	81	theme	0.027	1790:1794	arg1	s-1					1819:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	6	82	theme	GAG	1257:1259	arg1	microgels					1261:1269	GAG microgels	1257:1269	GAG microgels	1257:1269	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	6	83	theme	charge	1421:1426	arg1	density					1428:1434	increased local anionic charge density	1397:1434	increased local anionic charge density in the GAG networks	1397:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	3	84	theme	acid	498:501	arg1	HAMA					517:520	HAMA	517:520	HAMA	517:520	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	84	theme	acid	498:501	arg1	methacrylate					503:514	Monodisperse hyaluronic acid methacrylate	474:514	Monodisperse hyaluronic acid methacrylate (HAMA)	474:521	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	6	85	theme	local	1407:1411	arg1	density					1428:1434	increased local anionic charge density	1397:1434	increased local anionic charge density in the GAG networks	1397:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	10	86	theme	protein-matrix	2154:2167	arg1	interactions					2169:2180	weaker protein-matrix interactions	2147:2180	weaker protein-matrix interactions	2147:2180	Fluorescence Recovery After Photobleaching (FRAP) measurements yielded similar trends, although lysozyme diffusion was likely altered due to the negative charges introduced to the protein through the FITC-labeling resulting in weaker protein-matrix interactions.
30024582	7	87	from	factor	1561:1566	arg1	diameter					1573:1580	diameter	1573:1580	diameter	1573:1580	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	9	88	theme	<0.006	1808:1813	arg1	s-1					1819:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	0.027 in HAMA and <0.006 μm2 s-1	1790:1821	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	4	89	dep	microspheres	929:940	arg1	up					903:904	up	903:904	up	903:904	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	5	90	theme	Binding	1022:1028	arg1	stoichiometries					1030:1044	Binding stoichiometries	1022:1044	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.	1022:1228	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	7	91	theme	charged	1504:1510	arg1	networks					1521:1528	the negatively charged microgel networks	1489:1528	the negatively charged microgel networks	1489:1528	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	4	92	theme	20	867:868	arg1	mM					870:871	mM	870:871	mM	870:871	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	9	93	theme	s-1	1819:1821	arg1	coefficients					1774:1785	Lysozyme diffusion coefficients	1755:1785	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels	1755:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	3	94	theme	strengths	837:845	arg1	coefficient					761:771	its diffusion coefficient	747:771	its diffusion coefficient	747:771	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	94	theme	strengths	837:845	arg1	swelling					701:708	microgel (de)swelling	688:708	microgel (de)swelling	688:708	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	94	theme	strengths	837:845	arg1	distribution					730:741	intra-gel lysozyme distribution	711:741	intra-gel lysozyme distribution	711:741	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	94	theme	strengths	837:845	arg1	absorption					637:646	the absorption	633:646	the absorption of a cationic model protein (lysozyme)	633:685	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	0	95	from	microgels	113:121	arg1	loading					27:33	loading	27:33	loading	27:33	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	0	95	from	microgels	113:121	arg1	release					47:53	tunable release	39:53	tunable release of a cationic protein from monodisperse glycosaminoglycan microgels	39:121	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	9	96	theme	CSMA	1826:1829	arg1	microgels					1831:1839	CSMA microgels	1826:1839	CSMA microgels	1826:1839	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	5	97	theme	soluble	1196:1202	arg1	GAGs					1204:1207	soluble GAGs	1196:1207	soluble GAGs found in literature	1196:1227	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	6	98	theme	higher	1302:1307	arg1	strengths					1315:1323	higher ionic strengths	1302:1323	higher ionic strengths	1302:1323	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	3	99	theme	varying	823:829	arg1	strengths					837:845	varying ionic strengths	823:845	varying ionic strengths	823:845	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	7	100	theme	lysozyme	1477:1484	arg1	Binding					1457:1463	Binding	1457:1463	Binding of cationic lysozyme to the negatively charged microgel networks	1457:1528	Binding of cationic lysozyme to the negatively charged microgel networks resulted in deswelling up to a factor 2 in diameter.
30024582	4	101	theme	mg	917:918	arg1	microspheres					929:940	3 and 4 mg mg-1 dry microspheres	909:940	3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively	909:981	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	0	102	theme	monodisperse	82:93	arg1	microgels					113:121	monodisperse glycosaminoglycan microgels	82:121	monodisperse glycosaminoglycan microgels	82:121	Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels.
30024582	4	103	from	incubation	853:862	arg1	buffer					873:878	20 mM buffer	867:878	20 mM buffer	867:878	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	4	104	theme	dry	925:927	arg1	microspheres					929:940	3 and 4 mg mg-1 dry microspheres	909:940	3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively	909:981	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	3	105	from	coefficient	761:771	arg1	microgels					780:788	the microgels	776:788	the microgels dispersed in buffers (pH 7.4)	776:818	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	5	106	theme	complex	1167:1173	arg1	coacervates					1175:1185	GAG-lysozyme complex coacervates	1154:1185	GAG-lysozyme complex coacervates based on soluble GAGs found in literature	1154:1227	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	13	107	theme	release	2493:2499	arg1	depots					2501:2506	controlled release depots	2482:2506	controlled release depots	2482:2506	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	9	108	theme	mM	1859:1860	arg1	buffer					1862:1867	170 mM buffer	1855:1867	170 mM buffer (duration of release 14 and 28 days respectively)	1855:1917	Lysozyme diffusion coefficients of 0.027 in HAMA and <0.006 μm2 s-1 in CSMA microgels were found in 170 mM buffer (duration of release 14 and 28 days respectively).
30024582	8	109	theme	ionic	1640:1644	arg1	strength					1646:1653	the ionic strength	1636:1653	the ionic strength of the buffer	1636:1667	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	8	110	theme	groups	1698:1703	arg1	number					1680:1685	the number	1676:1685	the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA)	1676:1752	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	3	111	from	distribution	730:741	arg1	microgels					780:788	the microgels	776:788	the microgels dispersed in buffers (pH 7.4)	776:818	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	2	112	theme	release	397:403	arg1	kinetics					405:412	their protein release kinetics	383:412	their protein release kinetics upon encapsulation in hydrogel scaffolds	383:453	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	5	113	dep	10.2 	1074:1078	arg1	7.5 					1087:1090	7.5 	1087:1090	7.5 	1087:1090	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	5	113	dep	10.2 	1074:1078	arg1	 1					1080:1081	 1	1080:1081	 1	1080:1081	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	5	113	dep	10.2 	1074:1078	arg1	 1					1092:1093	 1	1092:1093	 1	1092:1093	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	11	114	theme	thermosensitive	2244:2258	arg1	scaffold					2269:2276	a thermosensitive hydrogel scaffold	2242:2276	a thermosensitive hydrogel scaffold	2242:2276	Finally, lysozyme-loaded CSMA microgels were embedded into a thermosensitive hydrogel scaffold.
30024582	6	115	theme	GAG-lysozyme	1339:1350	arg1	systems					1352:1358	GAG-lysozyme systems	1339:1358	GAG-lysozyme systems based on soluble GAGs	1339:1380	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	4	116	dep	100	1016:1018	arg1	to					1013:1014	to	1013:1014	to	1013:1014	Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%.
30024582	6	117	from	density	1428:1434	arg1	networks					1447:1454	the GAG networks	1439:1454	the GAG networks	1439:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	5	118	dep	 lysozyme	1063:1071	arg1	10.2 					1074:1078	10.2 	1074:1078	10.2 	1074:1078	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	3	119	theme	cationic	653:660	arg1	lysozyme					677:684	lysozyme	677:684	lysozyme	677:684	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	119	theme	cationic	653:660	arg1	protein					668:674	a cationic model protein	651:674	a cationic model protein (lysozyme)	651:685	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	13	120	theme	crosslinked	2424:2434	arg1	hydrogels					2454:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	covalently crosslinked methacrylated GAG hydrogels	2413:2462	In conclusion, covalently crosslinked methacrylated GAG hydrogels have potential as controlled release depots for cationic proteins in tissue engineering applications.
30024582	12	121	theme	composite	2285:2293	arg1	systems					2295:2301	These composite systems	2279:2301	These composite systems	2279:2301	These composite systems showed complete lysozyme release in ∼58 days as opposed to only 3 days for GAG-free scaffolds.
30024582	8	122	from	microgels	1609:1617	arg1	release					1592:1598	Lysozyme release	1583:1598	Lysozyme release from the microgels	1583:1617	Lysozyme release from the microgels was dependent on the ionic strength of the buffer and on the number of anionic groups per disaccharide, (1 for HAMA versus 2 for CSMA).
30024582	3	123	theme	hyaluronic	487:496	arg1	HAMA					517:520	HAMA	517:520	HAMA	517:520	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	123	theme	hyaluronic	487:496	arg1	methacrylate					503:514	Monodisperse hyaluronic acid methacrylate	474:514	Monodisperse hyaluronic acid methacrylate (HAMA)	474:521	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	6	124	theme	strengths	1315:1323	arg1	buffers					1291:1297	buffers	1291:1297	buffers of higher ionic strengths	1291:1323	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	5	125	dep	stoichiometries	1030:1044	arg1	similar					1133:1139	similar	1133:1139	similar	1133:1139	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	5	126	located	found	1209:1213	arg1	literature					1218:1227	literature	1218:1227	literature	1218:1227	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	5	126	located	found	1209:1213	arg2	GAGs					1204:1207	soluble GAGs	1196:1207	soluble GAGs found in literature	1196:1227	Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature.
30024582	2	127	theme	GAG-based	338:346	arg1	microgels					348:356	GAG-based microgels	338:356	GAG-based microgels	338:356	In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated.
30024582	6	128	theme	increased	1397:1405	arg1	density					1428:1434	increased local anionic charge density	1397:1434	increased local anionic charge density in the GAG networks	1397:1454	Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on soluble GAGs, likely due to increased local anionic charge density in the GAG networks.
30024582	3	129	theme	protein	668:674	arg1	coefficient					761:771	its diffusion coefficient	747:771	its diffusion coefficient	747:771	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	129	theme	protein	668:674	arg1	swelling					701:708	microgel (de)swelling	688:708	microgel (de)swelling	688:708	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	129	theme	protein	668:674	arg1	distribution					730:741	intra-gel lysozyme distribution	711:741	intra-gel lysozyme distribution	711:741	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	3	129	theme	protein	668:674	arg1	absorption					637:646	the absorption	633:646	the absorption of a cationic model protein (lysozyme)	633:685	Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths.
30024582	11	130	theme	CSMA	2208:2211	arg1	microgels					2213:2221	lysozyme-loaded CSMA microgels	2192:2221	lysozyme-loaded CSMA microgels	2192:2221	Finally, lysozyme-loaded CSMA microgels were embedded into a thermosensitive hydrogel scaffold.
31891702	8	0	theme	composite	1198:1206	arg1	scaffolds					1208:1216	ACGO composite scaffolds	1193:1216	ACGO composite scaffolds	1193:1216	These results suggest that ACGO composite scaffolds could be reliable and appropriate for tissue engineering applications.
31891702	1	1	dep	Three-dimensional	110:126	arg1	3D					129:130	3D	129:130	3D	129:130	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	4	2	theme	ACGO	593:596	arg1	scaffolds					608:616	The ACGO composite scaffolds	589:616	The ACGO composite scaffolds	589:616	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	3	3	theme	graphene	341:348	arg1	oxide					350:354	graphene oxide	341:354	graphene oxide (GO)	341:359	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	5	4	from	increase	816:823	arg1	content					831:837	GO content	828:837	GO content	828:837	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	5	theme	strength	512:519	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	6	6	theme	composite	923:931	arg1	scaffolds					933:941	The composite scaffolds	919:941	The composite scaffolds	919:941	The composite scaffolds showed adequate hemocompatibility and Vero cell proliferation ability.
31891702	7	7	theme	Cell	1014:1017	arg1	studies					1030:1036	Cell attachment studies	1014:1036	Cell attachment studies	1014:1036	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	3	8	theme	cytotoxicity	545:556	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	1	9	theme	Three-dimensional	110:126	arg1	scaffolds					140:148	Three-dimensional (3D) porous scaffolds	110:148	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite	110:206	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	7	10	dep	cell	1131:1134	arg1	attachment					1136:1145	attachment	1136:1145	attachment	1136:1145	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	3	11	theme	compressive	500:510	arg1	strength					512:519	compressive strength	500:519	compressive strength	500:519	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	4	12	theme	composite	598:606	arg1	scaffolds					608:616	The ACGO composite scaffolds	589:616	The ACGO composite scaffolds	589:616	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	3	13	theme	retention	489:497	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	7	14	from	present	1059:1065	arg1	scaffolds					1084:1092	the composite scaffolds	1070:1092	the composite scaffolds provided a favorable environment for cell attachment and proliferation	1070:1163	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	3	15	theme	degradation	532:542	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	8	16	theme	engineering	1263:1273	arg1	applications					1275:1286	tissue engineering applications	1256:1286	tissue engineering applications	1256:1286	These results suggest that ACGO composite scaffolds could be reliable and appropriate for tissue engineering applications.
31891702	5	17	theme	composite	777:785	arg1	scaffolds					787:795	the composite scaffolds	773:795	the composite scaffolds	773:795	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	18	theme	composite	417:425	arg1	scaffolds					427:435	the composite scaffolds	413:435	the composite scaffolds	413:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	5	19	dep	porosity	701:708	arg1	The					697:699	The	697:699	The	697:699	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	20	theme	biological	388:397	arg1	properties					399:408	the physicochemical and biological properties	364:408	the physicochemical and biological properties of the composite scaffolds	364:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	3	21	theme	enzymatic	522:530	arg1	degradation					532:542	enzymatic degradation	522:542	enzymatic degradation	522:542	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	3	22	theme	oxide	350:354	arg1	effect					331:336	The effect	327:336	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds	327:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	3	23	theme	behaviors	578:586	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	2	24	dep	FTIR	299:302	arg1	analysis					317:324	analysis	317:324	analysis	317:324	The prepared scaffolds were characterized by FTIR, XRD and SEM analysis.
31891702	0	25	theme	Three-dimensional	0:16	arg1	porous					18:23	Three-dimensional porous	0:23	Three-dimensional porous	0:23	Three-dimensional porous scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering.
31891702	5	26	theme	GO	915:916	arg1	addition					903:910	the addition	899:910	the addition of GO	899:916	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	27	theme	physicochemical	368:382	arg1	properties					399:408	the physicochemical and biological properties	364:408	the physicochemical and biological properties of the composite scaffolds	364:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	5	28	theme	retention	727:735	arg1	ability					737:743	water retention ability	721:743	water retention ability	721:743	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	29	theme	cell	562:565	arg1	behaviors					578:586	cell attachment behaviors	562:586	cell attachment behaviors	562:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	5	30	theme	degradation	850:860	arg1	rate					862:865	the degradation rate	846:865	the degradation rate of the scaffolds	846:882	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	7	31	theme	favorable	1105:1113	arg1	environment					1115:1125	a favorable environment	1103:1125	a favorable environment for cell attachment and proliferation	1103:1163	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	3	32	theme	attachment	567:576	arg1	behaviors					578:586	cell attachment behaviors	562:586	cell attachment behaviors	562:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	3	33	theme	swelling	473:480	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	0	34	theme	agarose/chitosan/graphene	44:68	arg1	composite					76:84	agarose/chitosan/graphene oxide composite	44:84	agarose/chitosan/graphene oxide composite for tissue engineering	44:107	Three-dimensional porous scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering.
31891702	6	35	theme	proliferation	991:1003	arg1	ability					1005:1011	Vero cell proliferation ability	981:1011	Vero cell proliferation ability	981:1011	The composite scaffolds showed adequate hemocompatibility and Vero cell proliferation ability.
31891702	3	36	dep	oxide	350:354	arg1	GO					357:358	GO	357:358	GO	357:358	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	4	37	theme	surface	677:683	arg1	morphology					685:694	rough surface morphology	671:694	rough surface morphology	671:694	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	5	38	theme	scaffolds	787:795	arg1	ability					737:743	water retention ability	721:743	water retention ability	721:743	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	5	38	theme	scaffolds	787:795	arg1	strength					761:768	compressive strength	749:768	compressive strength	749:768	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	5	38	theme	scaffolds	787:795	arg1	swelling					711:718	swelling	711:718	swelling	711:718	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	5	38	theme	scaffolds	787:795	arg1	porosity					701:708	porosity	701:708	porosity	701:708	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	1	39	theme	freeze-drying	229:241	arg1	technique					243:251	the freeze-drying technique	225:251	the freeze-drying technique	225:251	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	3	40	theme	scaffolds	427:435	arg1	properties					399:408	the physicochemical and biological properties	364:408	the physicochemical and biological properties of the composite scaffolds	364:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	6	41	theme	cell	986:989	arg1	ability					1005:1011	Vero cell proliferation ability	981:1011	Vero cell proliferation ability	981:1011	The composite scaffolds showed adequate hemocompatibility and Vero cell proliferation ability.
31891702	5	42	theme	compressive	749:759	arg1	strength					761:768	compressive strength	749:768	compressive strength	749:768	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	2	43	theme	prepared	258:265	arg1	scaffolds					267:275	The prepared scaffolds	254:275	The prepared scaffolds	254:275	The prepared scaffolds were characterized by FTIR, XRD and SEM analysis.
31891702	5	44	theme	scaffolds	874:882	arg1	rate					862:865	the degradation rate	846:865	the degradation rate of the scaffolds	846:882	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	3	45	theme	porosity	463:470	arg1	terms					454:458	terms	454:458	terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors	454:586	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	6	46	theme	Vero	981:984	arg1	ability					1005:1011	Vero cell proliferation ability	981:1011	Vero cell proliferation ability	981:1011	The composite scaffolds showed adequate hemocompatibility and Vero cell proliferation ability.
31891702	1	47	theme	porous	133:138	arg1	scaffolds					140:148	Three-dimensional (3D) porous scaffolds	110:148	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite	110:206	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	0	48	theme	oxide	70:74	arg1	composite					76:84	agarose/chitosan/graphene oxide composite	44:84	agarose/chitosan/graphene oxide composite for tissue engineering	44:107	Three-dimensional porous scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering.
31891702	5	49	dep	content	831:837	arg1	GO					828:829	GO	828:829	GO	828:829	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	8	50	theme	tissue	1256:1261	arg1	applications					1275:1286	tissue engineering applications	1256:1286	tissue engineering applications	1256:1286	These results suggest that ACGO composite scaffolds could be reliable and appropriate for tissue engineering applications.
31891702	7	51	theme	composite	1074:1082	arg1	scaffolds					1084:1092	the composite scaffolds	1070:1092	the composite scaffolds provided a favorable environment for cell attachment and proliferation	1070:1163	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	5	52	theme	water	721:725	arg1	ability					737:743	water retention ability	721:743	water retention ability	721:743	The porosity, swelling, water retention ability and compressive strength of the composite scaffolds increased with the increase in GO content, while the degradation rate of the scaffolds decreased with the addition of GO.
31891702	0	53	dep	scaffolds	25:33	arg1	based					35:39	based	35:39	scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering	25:107	Three-dimensional porous scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering.
31891702	3	54	theme	water	483:487	arg1	retention					489:497	water retention	483:497	water retention	483:497	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31891702	7	55	from	scaffolds	1084:1092	arg1	present					1059:1065	present	1059:1065	present	1059:1065	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	0	56	theme	tissue	90:95	arg1	engineering					97:107	tissue engineering	90:107	tissue engineering	90:107	Three-dimensional porous scaffolds based on agarose/chitosan/graphene oxide composite for tissue engineering.
31891702	4	57	theme	rough	671:675	arg1	morphology					685:694	rough surface morphology	671:694	rough surface morphology	671:694	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	1	58	theme	agarose/chitosan/graphene	159:183	arg1	oxide					185:189	agarose/chitosan/graphene oxide	159:189	agarose/chitosan/graphene oxide (ACGO) composite	159:206	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	1	58	theme	agarose/chitosan/graphene	159:183	arg1	ACGO					192:195	ACGO	192:195	ACGO	192:195	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	4	59	theme	interconnected	645:658	arg1	pores					660:664	the well-defined interconnected pores	628:664	the well-defined interconnected pores	628:664	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	8	60	theme	ACGO	1193:1196	arg1	scaffolds					1208:1216	ACGO composite scaffolds	1193:1216	ACGO composite scaffolds	1193:1216	These results suggest that ACGO composite scaffolds could be reliable and appropriate for tissue engineering applications.
31891702	6	61	theme	adequate	950:957	arg1	hemocompatibility					959:975	adequate hemocompatibility	950:975	adequate hemocompatibility	950:975	The composite scaffolds showed adequate hemocompatibility and Vero cell proliferation ability.
31891702	4	62	theme	well-defined	632:643	arg1	pores					660:664	the well-defined interconnected pores	628:664	the well-defined interconnected pores	628:664	The ACGO composite scaffolds exhibited the well-defined interconnected pores with rough surface morphology.
31891702	7	63	theme	attachment	1019:1028	arg1	studies					1030:1036	Cell attachment studies	1014:1036	Cell attachment studies	1014:1036	Cell attachment studies demonstrated that GO present in the composite scaffolds provided a favorable environment for cell attachment and proliferation.
31891702	1	64	theme	oxide	185:189	arg1	composite					198:206	agarose/chitosan/graphene oxide (ACGO) composite	159:206	agarose/chitosan/graphene oxide (ACGO) composite	159:206	Three-dimensional (3D) porous scaffolds based on agarose/chitosan/graphene oxide (ACGO) composite were prepared by the freeze-drying technique.
31891702	3	65	from	effect	331:336	arg1	properties					399:408	the physicochemical and biological properties	364:408	the physicochemical and biological properties of the composite scaffolds	364:435	The effect of graphene oxide (GO) on the physicochemical and biological properties of the composite scaffolds was evaluated in terms of porosity, swelling, water retention, compressive strength, enzymatic degradation, cytotoxicity and cell attachment behaviors.
31883887	6	0	theme	spectroscopic	936:948	arg1	analyses					950:957	Chemical and spectroscopic analyses	923:957	Chemical and spectroscopic analyses	923:957	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	3	1	theme	time	540:543	arg1	assay					545:549	the activated partial thromboplastin time assay	503:549	the activated partial thromboplastin time assay (0.54 IU/mg)	503:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	3	1	theme	time	540:543	arg1	0.54 IU/mg					552:561	0.54 IU/mg	552:561	0.54 IU/mg	552:561	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	4	2	theme	gel	631:633	arg1	chromatography					646:659	ethanol precipitation and gel filtration chromatography	605:659	chromatography	646:659	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	0	3	from	composition	15:25	arg1	extract					73:79	the aqueous extract	61:79	the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)	61:119	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	2	4	theme	ethnopharmacological	377:396	arg1	use					398:400	the ethnopharmacological use	373:400	the ethnopharmacological use	373:400	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	4	5	from	chromatography	646:659	arg1	G-50					673:676	Sephadex G-50	664:676	Sephadex G-50	664:676	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	5	from	chromatography	646:659	arg1	G-25					691:694	Sephadex G-25	682:694	Sephadex G-25	682:694	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	2	6	theme	antiophidic	301:311	arg1	properties					350:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	4	7	theme	water-soluble	710:722	arg1	fraction					724:731	a water-soluble fraction	708:731	a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg)	708:797	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	0	8	theme	chamaedrys	98:107	arg1	extract					73:79	the aqueous extract	61:79	the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)	61:119	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	4	9	dep	activity	762:769	arg1	F2-A					781:784	Fraction F2-A	772:784	Fraction F2-A; 2.94 IU/mg	772:796	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	10	theme	filtration	635:644	arg1	chromatography					646:659	ethanol precipitation and gel filtration chromatography	605:659	chromatography	646:659	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	5	11	theme	acids	862:866	arg1	potential					890:898	the anticoagulant potential	872:898	the anticoagulant potential of the active samples	872:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	11	theme	acids	862:866	arg1	amount					845:850	the amount	841:850	the amount of uronic acids	841:866	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	11	theme	acids	862:866	arg1	samples					914:920	the active samples	903:920	the active samples	903:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	11	theme	acids	862:866	arg1	acids					862:866	uronic acids	855:866	uronic acids	855:866	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	2	12	theme	chamaedrys	285:294	arg1	extracts					270:277	extracts	270:277	extracts of M. chamaedrys	270:294	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	1	13	theme	medicinal	164:172	arg1	chamaedrys					136:145	Marsypianthes chamaedrys	122:145	Marsypianthes chamaedrys (Lamiaceae)	122:157	Marsypianthes chamaedrys (Lamiaceae) is a medicinal plant popularly used against envenomation by snakebite.
31883887	1	13	theme	medicinal	164:172	arg1	plant					174:178	a medicinal plant	162:178	a medicinal plant popularly used against envenomation by snakebite	162:227	Marsypianthes chamaedrys (Lamiaceae) is a medicinal plant popularly used against envenomation by snakebite.
31883887	5	14	theme	positive	802:809	arg1	correlation					811:821	A positive correlation	800:821	A positive correlation	800:821	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	8	15	from	assay	1296:1300	arg1	extract					1259:1265	the aqueous extract	1247:1265	the aqueous extract of M. chamaedrys in the APTT assay	1247:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	2	16	theme	M.	282:283	arg1	chamaedrys					285:294	M. chamaedrys	282:294	M. chamaedrys	282:294	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	3	17	theme	chamaedrys	459:468	arg1	parts					447:451	aerial parts	440:451	aerial parts of M. chamaedrys	440:468	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	4	18	from	precipitation	613:625	arg1	G-50					673:676	Sephadex G-50	664:676	Sephadex G-50	664:676	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	18	from	precipitation	613:625	arg1	G-25					691:694	Sephadex G-25	682:694	Sephadex G-25	682:694	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	19	theme	ethanol	605:611	arg1	precipitation					613:625	ethanol precipitation and gel filtration chromatography	605:659	precipitation	613:625	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	8	20	theme	polysaccharides	1149:1163	arg1	arabinogalactans					1177:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	2	21	theme	anticoagulant	336:348	arg1	properties					350:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	3	22	theme	parts	447:451	arg1	extract					429:435	an aqueous extract	418:435	an aqueous extract of aerial parts of M. chamaedrys	418:468	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	0	23	theme	Polysaccharide	0:13	arg1	composition					15:25	Polysaccharide composition	0:25	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).	0:120	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	8	24	theme	Pectic	1142:1147	arg1	arabinogalactans					1177:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	5	25	theme	samples	914:920	arg1	potential					890:898	the anticoagulant potential	872:898	the anticoagulant potential of the active samples	872:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	25	theme	samples	914:920	arg1	amount					845:850	the amount	841:850	the amount of uronic acids	841:866	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	25	theme	samples	914:920	arg1	samples					914:920	the active samples	903:920	the active samples	903:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	5	25	theme	samples	914:920	arg1	acids					862:866	uronic acids	855:866	uronic acids	855:866	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	6	26	theme	Chemical	923:930	arg1	analyses					950:957	Chemical and spectroscopic analyses	923:957	Chemical and spectroscopic analyses	923:957	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	8	27	theme	extract	1259:1265	arg1	activity					1235:1242	the anticoagulant activity	1217:1242	the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay	1217:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	8	28	theme	type	1169:1172	arg1	arabinogalactans					1177:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans	1142:1192	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	2	29	theme	anti-inflammatory	314:330	arg1	properties					350:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	5	30	theme	anticoagulant	876:888	arg1	potential					890:898	the anticoagulant potential	872:898	the anticoagulant potential of the active samples	872:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	2	31	contain	have	296:299	arg2	properties					350:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	antiophidic, anti-inflammatory and anticoagulant properties	301:359	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	2	31	contain	have	296:299	arg1	extracts					270:277	extracts	270:277	extracts of M. chamaedrys	270:294	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	7	32	theme	FT-IR	1079:1083	arg1	spectra					1085:1091	UV and FT-IR spectra	1072:1091	spectra	1085:1091	UV and FT-IR spectra indicated the possible presence of ferulic acid.
31883887	4	33	theme	Sephadex	682:689	arg1	G-25					691:694	Sephadex G-25	682:694	Sephadex G-25	682:694	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	0	34	theme	fraction	47:54	arg1	composition					15:25	Polysaccharide composition	0:25	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).	0:120	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	3	35	theme	thromboplastin	525:538	arg1	time					540:543	the activated partial thromboplastin time	503:543	the activated partial thromboplastin time assay (0.54 IU/mg)	503:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	7	36	theme	acid	1136:1139	arg1	presence					1116:1123	the possible presence	1103:1123	the possible presence of ferulic acid	1103:1139	UV and FT-IR spectra indicated the possible presence of ferulic acid.
31883887	4	37	with	fraction	724:731	arg1	activity					762:769	increased anticoagulant activity	738:769	increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg)	738:797	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	6	38	theme	type	1034:1037	arg1	arabinogalactan					1042:1056	type II arabinogalactan	1034:1056	type II arabinogalactan	1034:1056	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	0	39	theme	anticoagulant	33:45	arg1	fraction					47:54	an anticoagulant fraction	30:54	an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)	30:119	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	3	40	theme	aerial	440:445	arg1	parts					447:451	aerial parts	440:451	aerial parts of M. chamaedrys	440:468	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	0	41	from	extract	73:79	arg1	fraction					47:54	an anticoagulant fraction	30:54	an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)	30:119	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	0	41	from	extract	73:79	arg1	composition					15:25	Polysaccharide composition	0:25	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).	0:120	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	3	42	theme	anticoagulant	477:489	arg1	activity					491:498	anticoagulant activity	477:498	anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg)	477:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	3	43	theme	aqueous	421:427	arg1	extract					429:435	an aqueous extract	418:435	an aqueous extract of aerial parts of M. chamaedrys	418:468	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	8	44	theme	aqueous	1251:1257	arg1	extract					1259:1265	the aqueous extract	1247:1265	the aqueous extract of M. chamaedrys in the APTT assay	1247:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	8	45	theme	APTT	1291:1294	arg1	assay					1296:1300	the APTT assay	1287:1300	the APTT assay	1287:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	3	46	theme	M.	456:457	arg1	chamaedrys					459:468	M. chamaedrys	456:468	M. chamaedrys	456:468	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	2	47	theme	Pharmacological	230:244	arg1	studies					246:252	Pharmacological studies	230:252	Pharmacological studies	230:252	Pharmacological studies have shown that extracts of M. chamaedrys have antiophidic, anti-inflammatory and anticoagulant properties, supporting the ethnopharmacological use.
31883887	3	48	theme	partial	517:523	arg1	time					540:543	the activated partial thromboplastin time	503:543	the activated partial thromboplastin time assay (0.54 IU/mg)	503:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	5	49	theme	uronic	855:860	arg1	acids					862:866	uronic acids	855:866	uronic acids	855:866	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	4	50	theme	bioassay-guided	569:583	arg1	fractionation					585:597	The bioassay-guided fractionation	565:597	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25	565:694	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	7	51	theme	ferulic	1128:1134	arg1	acid					1136:1139	ferulic acid	1128:1139	ferulic acid	1128:1139	UV and FT-IR spectra indicated the possible presence of ferulic acid.
31883887	3	52	from	activity	491:498	arg1	assay					545:549	the activated partial thromboplastin time assay	503:549	the activated partial thromboplastin time assay (0.54 IU/mg)	503:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	3	52	from	activity	491:498	arg1	0.54 IU/mg					552:561	0.54 IU/mg	552:561	0.54 IU/mg	552:561	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	1	53	theme	Marsypianthes	122:134	arg1	chamaedrys					136:145	Marsypianthes chamaedrys	122:145	Marsypianthes chamaedrys (Lamiaceae)	122:157	Marsypianthes chamaedrys (Lamiaceae) is a medicinal plant popularly used against envenomation by snakebite.
31883887	1	53	theme	Marsypianthes	122:134	arg1	plant					174:178	a medicinal plant	162:178	a medicinal plant popularly used against envenomation by snakebite	162:227	Marsypianthes chamaedrys (Lamiaceae) is a medicinal plant popularly used against envenomation by snakebite.
31883887	1	53	theme	Marsypianthes	122:134	arg1	Lamiaceae					148:156	Lamiaceae	148:156	Lamiaceae	148:156	Marsypianthes chamaedrys (Lamiaceae) is a medicinal plant popularly used against envenomation by snakebite.
31883887	6	54	theme	type	1007:1010	arg1	rhamnogalacturonan					1014:1031	type I rhamnogalacturonan	1007:1031	type I rhamnogalacturonan	1007:1031	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	7	55	theme	possible	1107:1114	arg1	presence					1116:1123	the possible presence	1103:1123	the possible presence of ferulic acid	1103:1139	UV and FT-IR spectra indicated the possible presence of ferulic acid.
31883887	8	56	from	extract	1259:1265	arg1	assay					1296:1300	the APTT assay	1287:1300	the APTT assay	1287:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	4	57	dep	F2-A	781:784	arg1	2.94 IU/mg					787:796	2.94 IU/mg	787:796	Fraction F2-A; 2.94 IU/mg	772:796	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	58	theme	Sephadex	664:671	arg1	G-50					673:676	Sephadex G-50	664:676	Sephadex G-50	664:676	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	7	59	theme	UV	1072:1073	arg1	spectra					1085:1091	UV and FT-IR spectra	1072:1091	spectra	1085:1091	UV and FT-IR spectra indicated the possible presence of ferulic acid.
31883887	3	60	theme	activated	507:515	arg1	time					540:543	the activated partial thromboplastin time	503:543	the activated partial thromboplastin time assay (0.54 IU/mg)	503:562	In this study, an aqueous extract of aerial parts of M. chamaedrys showed anticoagulant activity in the activated partial thromboplastin time assay (0.54 IU/mg).
31883887	8	61	theme	chamaedrys	1273:1282	arg1	extract					1259:1265	the aqueous extract	1247:1265	the aqueous extract of M. chamaedrys in the APTT assay	1247:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	8	62	theme	anticoagulant	1221:1233	arg1	activity					1235:1242	the anticoagulant activity	1217:1242	the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay	1217:1300	Pectic polysaccharides and type II arabinogalactans may be contributing to the anticoagulant activity of the aqueous extract of M. chamaedrys in the APTT assay.
31883887	5	63	theme	active	907:912	arg1	samples					914:920	the active samples	903:920	the active samples	903:920	A positive correlation was found between the amount of uronic acids and the anticoagulant potential of the active samples.
31883887	4	64	theme	anticoagulant	748:760	arg1	activity					762:769	increased anticoagulant activity	738:769	increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg)	738:797	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	6	65	contain	contained	979:987	arg2	α-glucan					1062:1069	α-glucan	1062:1069	α-glucan	1062:1069	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	6	65	contain	contained	979:987	arg2	arabinogalactan					1042:1056	type II arabinogalactan	1034:1056	type II arabinogalactan	1034:1056	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	6	65	contain	contained	979:987	arg2	rhamnogalacturonan					1014:1031	type I rhamnogalacturonan	1007:1031	type I rhamnogalacturonan	1007:1031	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	6	65	contain	contained	979:987	arg1	F2-A					974:977	F2-A	974:977	F2-A	974:977	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	6	65	contain	contained	979:987	arg2	homogalacturonan					989:1004	homogalacturonan	989:1004	homogalacturonan	989:1004	Chemical and spectroscopic analyses indicated that F2-A contained homogalacturonan, type I rhamnogalacturonan, type II arabinogalactan and α-glucan.
31883887	0	66	theme	aqueous	65:71	arg1	extract					73:79	the aqueous extract	61:79	the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)	61:119	Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae).
31883887	4	67	theme	increased	738:746	arg1	activity					762:769	increased anticoagulant activity	738:769	increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg)	738:797	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
31883887	4	68	theme	Fraction	772:779	arg1	F2-A					781:784	Fraction F2-A	772:784	Fraction F2-A; 2.94 IU/mg	772:796	The bioassay-guided fractionation using ethanol precipitation and gel filtration chromatography on Sephadex G-50 and Sephadex G-25 resulted in a water-soluble fraction with increased anticoagulant activity (Fraction F2-A; 2.94 IU/mg).
29514453	1	0	theme	gut	628:630	arg1	microbiota					632:641	gut microbiota	628:641	gut microbiota	628:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	1	theme	increasing	150:159	arg1	evidence					171:178	evidence	171:178	evidence	171:178	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	1	theme	increasing	150:159	arg1	amount					161:166	An increasing amount	147:166	An increasing amount of evidence	147:178	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	3	2	theme	phylogenetic	824:835	arg1	diversity					837:845	the phylogenetic diversity	820:845	the phylogenetic diversity of HFD-induced microbiota	820:871	Based on results of sequencing, FBTPS treatment could increase the phylogenetic diversity of HFD-induced microbiota.
29514453	0	3	theme	Induced	80:86	arg1	Mice					88:91	High-Fat Diet Induced Mice	66:91	High-Fat Diet Induced Mice	66:91	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	5	4	with	association	1231:1241	arg1	modulation					1252:1261	the modulation	1248:1261	the modulation of gut microbiota	1248:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	5	5	theme	key	1082:1084	arg1	OTUs					1086:1089	44 key OTUs	1079:1089	44 key OTUs	1079:1089	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	5	6	theme	novel	1191:1195	arg1	candidate					1197:1205	a novel candidate	1189:1205	a novel candidate for prevention of MS in association with the modulation of gut microbiota	1189:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	5	6	theme	novel	1191:1195	arg1	FBTPS					1168:1172	FBTPS	1168:1172	FBTPS	1168:1172	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	4	7	theme	relative	950:957	arg1	abundances					959:968	relative abundances	950:968	relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	950:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	1	8	theme	new	333:335	arg1	target					337:342	a new target	331:342	a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	331:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	8	theme	new	333:335	arg1	forward					320:326	forward	320:326	forward	320:326	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	3	9	theme	sequencing	777:786	arg1	results					766:772	results	766:772	results of sequencing	766:786	Based on results of sequencing, FBTPS treatment could increase the phylogenetic diversity of HFD-induced microbiota.
29514453	1	10	theme	gut	482:484	arg1	dysbiosis					497:505	gut microbiota dysbiosis	482:505	gut microbiota dysbiosis	482:505	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	11	theme	metabolic	276:284	arg1	MS					296:297	MS	296:297	MS	296:297	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	11	theme	metabolic	276:284	arg1	syndrome					286:293	metabolic syndrome	276:293	metabolic syndrome (MS)	276:298	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	4	12	theme	HFD-induced	925:935	arg1	increases					937:945	the HFD-induced increases	921:945	the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	921:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	0	13	from	Syndrome	54:61	arg1	Mice					88:91	High-Fat Diet Induced Mice	66:91	High-Fat Diet Induced Mice	66:91	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	1	14	theme	microbiota	486:495	arg1	dysbiosis					497:505	gut microbiota dysbiosis	482:505	gut microbiota dysbiosis	482:505	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	15	from	MS	475:476	arg1	mice					534:537	high-fat diet (HFD) fed mice	510:537	high-fat diet (HFD) fed mice	510:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	4	16	theme	Erysipelotrichaceae	973:991	arg1	abundances					959:968	relative abundances	950:968	relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	950:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	2	17	theme	FBTPS	668:672	arg1	intervention					674:685	FBTPS intervention	668:685	FBTPS intervention	668:685	The results showed that FBTPS intervention could significantly attenuate metabolic syndrome in HFD-induced mice.
29514453	1	18	theme	evidence	171:178	arg1	evidence					171:178	evidence	171:178	evidence	171:178	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	18	theme	evidence	171:178	arg1	amount					161:166	An increasing amount	147:166	An increasing amount of evidence	147:178	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	19	theme	Brick	8:12	arg1	Polysaccharides					18:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	1	20	theme	microbiota	632:641	arg1	modulation					614:623	the modulation	610:623	the modulation of gut microbiota	610:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	21	theme	Fuzhuan	0:6	arg1	Polysaccharides					18:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	1	22	theme	syndrome	286:293	arg1	pathophysiology					257:271	the pathophysiology	253:271	the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	253:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	23	theme	Fuzhuan	430:436	arg1	FBTPS					465:469	FBTPS	465:469	FBTPS	465:469	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	23	theme	Fuzhuan	430:436	arg1	polysaccharides					448:462	Fuzhuan brick tea polysaccharides	430:462	Fuzhuan brick tea polysaccharides (FBTPS)	430:470	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	24	theme	high-fat	510:517	arg1	diet					519:522	high-fat diet	510:522	high-fat diet (HFD) fed mice	510:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	24	theme	high-fat	510:517	arg1	HFD					525:527	HFD	525:527	HFD	525:527	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	25	with	Association	96:106	arg1	Modulation					113:122	Modulation	113:122	Modulation in the Gut Microbiota	113:144	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	4	26	theme	FBTPS	874:878	arg1	intervention					880:891	FBTPS intervention	874:891	FBTPS intervention	874:891	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	1	27	theme	brick	438:442	arg1	FBTPS					465:469	FBTPS	465:469	FBTPS	465:469	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	27	theme	brick	438:442	arg1	polysaccharides					448:462	Fuzhuan brick tea polysaccharides	430:462	Fuzhuan brick tea polysaccharides (FBTPS)	430:470	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	28	theme	diet	519:522	arg1	mice					534:537	high-fat diet (HFD) fed mice	510:537	high-fat diet (HFD) fed mice	510:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	29	theme	Tea	14:16	arg1	Polysaccharides					18:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides	0:32	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	3	30	theme	HFD-induced	850:860	arg1	microbiota					862:871	HFD-induced microbiota	850:871	HFD-induced microbiota	850:871	Based on results of sequencing, FBTPS treatment could increase the phylogenetic diversity of HFD-induced microbiota.
29514453	5	31	theme	microbiota	1270:1279	arg1	modulation					1252:1261	the modulation	1248:1261	the modulation of gut microbiota	1248:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	1	32	theme	gut	198:200	arg1	composition					213:223	the gut microbiota composition	194:223	the gut microbiota composition	194:223	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	33	theme	Metabolic	44:52	arg1	Syndrome					54:61	Metabolic Syndrome	44:61	Metabolic Syndrome in High-Fat Diet Induced Mice	44:91	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	3	34	theme	microbiota	862:871	arg1	diversity					837:845	the phylogenetic diversity	820:845	the phylogenetic diversity of HFD-induced microbiota	820:871	Based on results of sequencing, FBTPS treatment could increase the phylogenetic diversity of HFD-induced microbiota.
29514453	1	35	theme	microbiota	202:211	arg1	composition					213:223	the gut microbiota composition	194:223	the gut microbiota composition	194:223	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	36	from	target	337:342	arg1	treatment					351:359	the treatment	347:359	the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	347:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	37	theme	Gut	131:133	arg1	Microbiota					135:144	the Gut Microbiota	127:144	the Gut Microbiota	127:144	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	1	38	theme	diet-induced	364:375	arg1	MS.					377:379	diet-induced MS.	364:379	diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	364:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	39	theme	tea	444:446	arg1	FBTPS					465:469	FBTPS	465:469	FBTPS	465:469	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	39	theme	tea	444:446	arg1	polysaccharides					448:462	Fuzhuan brick tea polysaccharides	430:462	Fuzhuan brick tea polysaccharides (FBTPS)	430:470	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	4	40	theme	Coriobacteriaceae	994:1010	arg1	abundances					959:968	relative abundances	950:968	relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	950:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	1	41	theme	MS.	377:379	arg1	treatment					351:359	the treatment	347:359	the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	347:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	42	theme	polysaccharides	448:462	arg1	effects					419:425	effects	419:425	effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice	419:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	0	43	from	Modulation	113:122	arg1	Microbiota					135:144	the Gut Microbiota	127:144	the Gut Microbiota	127:144	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	3	44	theme	FBTPS	789:793	arg1	treatment					795:803	FBTPS treatment	789:803	FBTPS treatment	789:803	Based on results of sequencing, FBTPS treatment could increase the phylogenetic diversity of HFD-induced microbiota.
29514453	5	45	from	prevention	1211:1220	arg1	association					1231:1241	association	1231:1241	association with the modulation of gut microbiota	1231:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	4	46	theme	Streptococcaceae	1017:1032	arg1	abundances					959:968	relative abundances	950:968	relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	950:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
29514453	1	47	from	dysbiosis	497:505	arg1	mice					534:537	high-fat diet (HFD) fed mice	510:537	high-fat diet (HFD) fed mice	510:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	48	from	effects	419:425	arg1	dysbiosis					497:505	gut microbiota dysbiosis	482:505	gut microbiota dysbiosis	482:505	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	48	from	effects	419:425	arg1	MS					475:476	MS	475:476	MS	475:476	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	1	49	theme	fed	530:532	arg1	mice					534:537	high-fat diet (HFD) fed mice	510:537	high-fat diet (HFD) fed mice	510:537	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	5	50	theme	correlation	1046:1056	arg1	analysis					1058:1065	Spearman's correlation analysis	1035:1065	Spearman's correlation analysis	1035:1065	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	2	51	theme	metabolic	717:725	arg1	syndrome					727:734	metabolic syndrome	717:734	metabolic syndrome in HFD-induced mice	717:754	The results showed that FBTPS intervention could significantly attenuate metabolic syndrome in HFD-induced mice.
29514453	2	52	theme	HFD-induced	739:749	arg1	mice					751:754	HFD-induced mice	739:754	HFD-induced mice	739:754	The results showed that FBTPS intervention could significantly attenuate metabolic syndrome in HFD-induced mice.
29514453	1	53	dep	MS.	377:379	arg1	aimed					398:402	aimed	398:402	aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota	398:641	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	2	54	from	syndrome	727:734	arg1	mice					751:754	HFD-induced mice	739:754	HFD-induced mice	739:754	The results showed that FBTPS intervention could significantly attenuate metabolic syndrome in HFD-induced mice.
29514453	5	55	theme	gut	1266:1268	arg1	microbiota					1270:1279	gut microbiota	1266:1279	gut microbiota	1266:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	5	56	theme	MS	1225:1226	arg1	prevention					1211:1220	prevention	1211:1220	prevention of MS in association with the modulation of gut microbiota	1211:1279	Spearman's correlation analysis showed that 44 key OTUs were negatively or positively associated with MS. Our results suggested that FBTPS could serve as a novel candidate for prevention of MS in association with the modulation of gut microbiota.
29514453	0	57	theme	High-Fat	66:73	arg1	Diet					75:78	High-Fat Diet	66:78	High-Fat Diet	66:78	Fuzhuan Brick Tea Polysaccharides Attenuate Metabolic Syndrome in High-Fat Diet Induced Mice in Association with Modulation in the Gut Microbiota.
29514453	1	58	theme	MS	593:594	arg1	attenuation					578:588	its attenuation	574:588	its attenuation of MS	574:594	An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota.
29514453	4	59	from	increases	937:945	arg1	abundances					959:968	relative abundances	950:968	relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae	950:1032	FBTPS intervention could significantly restore the HFD-induced increases in relative abundances of Erysipelotrichaceae, Coriobacteriaceae, and Streptococcaceae.
31323374	2	0	from	therapy	448:454	arg1	treatment					463:471	the treatment	459:471	the treatment of many inflammatory diseases	459:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	4	1	theme	controlled	716:725	arg1	evaporation					727:737	controlled evaporation	716:737	controlled evaporation of the organic solvent	716:760	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	9	2	theme	incorporation	1515:1527	arg1	effect					1501:1506	a beneficial effect	1488:1506	a beneficial effect of the incorporation of curcumin into GPs	1488:1548	The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
31323374	1	3	theme	hollow	157:162	arg1	particles					107:115	Glucan particles	100:115	Glucan particles (GPs) from Saccharomyces cerevisiae	100:151	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	3	theme	hollow	157:162	arg1	shells					164:169	hollow shells	157:169	hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo	157:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	4	theme	anti-inflammatory	259:275	arg1	potential					277:285	immunomodulatory and anti-inflammatory potential	238:285	immunomodulatory and anti-inflammatory potential both in vitro and in vivo	238:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	0	5	from	encapsulation	9:21	arg1	particles					39:47	yeast glucan particles	26:47	yeast glucan particles	26:47	Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro.
31323374	7	6	theme	GPs	1228:1230	arg1	activity					1210:1217	a pro-oxidative activity	1194:1217	a pro-oxidative activity of empty GPs	1194:1230	Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect.
31323374	3	7	theme	study	520:524	arg1	aim					508:510	The aim	504:510	The aim of this study	504:524	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	7	8	theme	empty	1222:1226	arg1	GPs					1228:1230	empty GPs	1222:1230	empty GPs	1222:1230	Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect.
31323374	6	9	theme	pro-inflammatory	1106:1121	arg1	cytokines					1123:1131	pro-inflammatory cytokines TNF-α and IL-1β	1106:1147	pro-inflammatory cytokines TNF-α and IL-1β	1106:1147	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	6	9	theme	pro-inflammatory	1106:1121	arg1	IL-1β					1143:1147	IL-1β	1143:1147	IL-1β	1143:1147	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	6	9	theme	pro-inflammatory	1106:1121	arg1	TNF-α					1133:1137	TNF-α	1133:1137	TNF-α	1133:1137	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	3	10	theme	possible	545:552	arg1	effect					563:568	the possible synergic effect	541:568	the possible synergic effect	541:568	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	11	from	form	638:641	arg1	effect					563:568	the possible synergic effect	541:568	the possible synergic effect	541:568	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	11	from	form	638:641	arg1	benefits					580:587	other benefits	574:587	other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites	574:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	1	12	from	cerevisiae	142:151	arg1	GPs					118:120	GPs	118:120	GPs	118:120	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	12	from	cerevisiae	142:151	arg1	particles					107:115	Glucan particles	100:115	Glucan particles (GPs) from Saccharomyces cerevisiae	100:151	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	12	from	cerevisiae	142:151	arg1	shells					164:169	hollow shells	157:169	hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo	157:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	3	13	theme	synergic	554:561	arg1	effect					563:568	the possible synergic effect	541:568	the possible synergic effect	541:568	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	1	14	theme	in	292:293	arg1	potential					277:285	immunomodulatory and anti-inflammatory potential	238:285	immunomodulatory and anti-inflammatory potential both in vitro and in vivo	238:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	5	15	theme	anti-oxidative	893:906	arg1	effect					908:913	The anti-oxidative effect	889:913	The anti-oxidative effect	889:913	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	8	16	theme	tested	1369:1374	arg1	composites					1388:1397	the tested GP/curcumin composites	1365:1397	the tested GP/curcumin composites	1365:1397	The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion.
31323374	5	17	theme	signaling	986:994	arg1	pathway					996:1002	the NF-κB/AP-1 signaling pathway	971:1002	the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes	971:1046	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	2	18	contain	possesses	373:381	arg1	Curcumin					314:321	Curcumin	314:321	Curcumin	314:321	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	18	contain	possesses	373:381	arg1	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	18	contain	possesses	373:381	arg1	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	18	contain	possesses	373:381	arg2	effect					415:420	a significant anti-inflammatory effect	383:420	a significant anti-inflammatory effect	383:420	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	7	19	theme	pro-oxidative	1196:1208	arg1	activity					1210:1217	a pro-oxidative activity	1194:1217	a pro-oxidative activity of empty GPs	1194:1230	Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect.
31323374	4	20	theme	anti-inflammatory	798:814	arg1	potential					816:824	anti-inflammatory potential	798:824	anti-inflammatory potential	798:824	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	3	21	from	curcumin	622:629	arg1	form					638:641	the form	634:641	the form of pharmaceutical composites	634:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	6	22	dep	cytokines	1123:1131	arg1	cytokines					1123:1131	pro-inflammatory cytokines TNF-α and IL-1β	1106:1147	pro-inflammatory cytokines TNF-α and IL-1β	1106:1147	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	6	22	dep	cytokines	1123:1131	arg1	IL-1β					1143:1147	IL-1β	1143:1147	IL-1β	1143:1147	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	6	22	dep	cytokines	1123:1131	arg1	TNF-α					1133:1137	TNF-α	1133:1137	TNF-α	1133:1137	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	5	23	from	pathway	996:1002	arg1	monocytes					1038:1046	lipopolysaccharide pre-treated monocytes	1007:1046	lipopolysaccharide pre-treated monocytes	1007:1046	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	3	24	theme	other	574:578	arg1	benefits					580:587	other benefits	574:587	other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites	574:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	2	25	theme	anti-inflammatory	397:413	arg1	effect					415:420	a significant anti-inflammatory effect	383:420	a significant anti-inflammatory effect	383:420	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	0	26	theme	Curcumin	0:7	arg1	encapsulation					9:21	Curcumin encapsulation	0:21	Curcumin encapsulation in yeast glucan particles	0:47	Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro.
31323374	7	27	theme	pharmaceutical	1242:1255	arg1	composites					1257:1266	pharmaceutical composites	1242:1266	pharmaceutical composites	1242:1266	Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect.
31323374	8	28	theme	NF-κB/AP-1	1323:1332	arg1	activity					1311:1318	The activity	1307:1318	The activity of NF-κB/AP-1	1307:1332	The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion.
31323374	1	29	theme	Glucan	100:105	arg1	GPs					118:120	GPs	118:120	GPs	118:120	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	29	theme	Glucan	100:105	arg1	particles					107:115	Glucan particles	100:115	Glucan particles (GPs) from Saccharomyces cerevisiae	100:151	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	1	29	theme	Glucan	100:105	arg1	shells					164:169	hollow shells	157:169	hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo	157:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	2	30	theme	significant	385:395	arg1	effect					415:420	a significant anti-inflammatory effect	383:420	a significant anti-inflammatory effect	383:420	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	0	31	theme	yeast	26:30	arg1	particles					39:47	yeast glucan particles	26:47	yeast glucan particles	26:47	Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro.
31323374	9	32	theme	obtained	1462:1469	arg1	results					1471:1477	The obtained results	1458:1477	The obtained results	1458:1477	The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
31323374	4	33	theme	anti-oxidative	772:785	arg1	effect					787:792	their anti-oxidative effect	766:792	their anti-oxidative effect	766:792	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	2	34	theme	supportive	437:446	arg1	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	34	theme	supportive	437:446	arg1	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	4	35	theme	solvent	754:760	arg1	evaporation					727:737	controlled evaporation	716:737	controlled evaporation of the organic solvent	716:760	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	1	36	theme	in	305:306	arg1	potential					277:285	immunomodulatory and anti-inflammatory potential	238:285	immunomodulatory and anti-inflammatory potential both in vitro and in vivo	238:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	5	37	theme	lipopolysaccharide	1007:1024	arg1	monocytes					1038:1046	lipopolysaccharide pre-treated monocytes	1007:1046	lipopolysaccharide pre-treated monocytes	1007:1046	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	8	38	theme	secretion	1447:1455	arg1	attenuation					1422:1432	the attenuation	1418:1432	the attenuation of cytokines secretion	1418:1455	The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion.
31323374	3	39	from	co-application	596:609	arg1	form					638:641	the form	634:641	the form of pharmaceutical composites	634:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	8	40	theme	GP/curcumin	1376:1386	arg1	composites					1388:1397	the tested GP/curcumin composites	1365:1397	the tested GP/curcumin composites	1365:1397	The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion.
31323374	2	41	theme	diseases	494:501	arg1	treatment					463:471	the treatment	459:471	the treatment of many inflammatory diseases	459:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	5	42	theme	NF-κB/AP-1	975:984	arg1	pathway					996:1002	the NF-κB/AP-1 signaling pathway	971:1002	the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes	971:1046	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	3	43	theme	pharmaceutical	646:659	arg1	composites					661:670	pharmaceutical composites	646:670	pharmaceutical composites	646:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	44	theme	co-application	596:609	arg1	effect					563:568	the possible synergic effect	541:568	the possible synergic effect	541:568	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	44	theme	co-application	596:609	arg1	benefits					580:587	other benefits	574:587	other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites	574:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	4	45	theme	GP/curcumin	673:683	arg1	composites					685:694	GP/curcumin composites	673:694	GP/curcumin composites	673:694	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	4	46	dep	monocytes	868:876	arg1	line					883:886	cell line	878:886	THP1‑XBlue™‑MD2‑CD14 human monocytes cell line	841:886	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	0	47	theme	glucan	32:37	arg1	particles					39:47	yeast glucan particles	26:47	yeast glucan particles	26:47	Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro.
31323374	2	48	theme	inflammatory	481:492	arg1	diseases					494:501	many inflammatory diseases	476:501	many inflammatory diseases	476:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	4	49	theme	human	862:866	arg1	monocytes					868:876	THP1‑XBlue™‑MD2‑CD14 human monocytes	841:876	THP1‑XBlue™‑MD2‑CD14 human monocytes cell line	841:886	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	2	50	theme	many	476:479	arg1	diseases					494:501	many inflammatory diseases	476:501	many inflammatory diseases	476:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	9	51	theme	beneficial	1490:1499	arg1	effect					1501:1506	a beneficial effect	1488:1506	a beneficial effect of the incorporation of curcumin into GPs	1488:1548	The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
31323374	2	52	used	used	429:432	arg2	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	52	used	used	429:432	arg2	Curcumin					314:321	Curcumin	314:321	Curcumin	314:321	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	52	used	used	429:432	arg2	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	5	53	theme	pre-treated	1026:1036	arg1	monocytes					1038:1046	lipopolysaccharide pre-treated monocytes	1007:1046	lipopolysaccharide pre-treated monocytes	1007:1046	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	4	54	theme	cell	878:881	arg1	line					883:886	cell line	878:886	THP1‑XBlue™‑MD2‑CD14 human monocytes cell line	841:886	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	2	55	theme	phenolic	348:355	arg1	Curcumin					314:321	Curcumin	314:321	Curcumin	314:321	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	55	theme	phenolic	348:355	arg1	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	55	theme	phenolic	348:355	arg1	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	3	56	from	effect	563:568	arg1	form					638:641	the form	634:641	the form of pharmaceutical composites	634:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	1	57	dep	in	292:293	arg1	vitro					295:299	vitro	295:299	vitro	295:299	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	3	58	theme	curcumin	622:629	arg1	effect					563:568	the possible synergic effect	541:568	the possible synergic effect	541:568	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	58	theme	curcumin	622:629	arg1	benefits					580:587	other benefits	574:587	other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites	574:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	6	59	theme	cytokines	1123:1131	arg1	well					1166:1169	well	1166:1169	well	1166:1169	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	6	59	theme	cytokines	1123:1131	arg1	secretion					1093:1101	The secretion	1089:1101	The secretion of pro-inflammatory cytokines TNF-α and IL-1β	1089:1147	The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well.
31323374	2	60	theme	hydrophobic	336:346	arg1	Curcumin					314:321	Curcumin	314:321	Curcumin	314:321	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	60	theme	hydrophobic	336:346	arg1	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	60	theme	hydrophobic	336:346	arg1	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	5	61	theme	pyocyanin-stimulated	931:950	arg1	cells					952:956	pyocyanin-stimulated cells	931:956	pyocyanin-stimulated cells	931:956	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	5	62	theme	anti-inflammatory	1063:1079	arg1	assays					1081:1086	anti-inflammatory assays	1063:1086	anti-inflammatory assays	1063:1086	The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays.
31323374	3	63	theme	composites	661:670	arg1	form					638:641	the form	634:641	the form of pharmaceutical composites	634:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	2	64	theme	natural	328:334	arg1	Curcumin					314:321	Curcumin	314:321	Curcumin	314:321	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	64	theme	natural	328:334	arg1	therapy					448:454	supportive therapy	437:454	supportive therapy in the treatment of many inflammatory diseases	437:501	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	2	64	theme	natural	328:334	arg1	compound					357:364	a natural hydrophobic phenolic compound	326:364	a natural hydrophobic phenolic compound	326:364	Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases.
31323374	4	65	theme	organic	746:752	arg1	solvent					754:760	the organic solvent	742:760	the organic solvent	742:760	GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line.
31323374	3	66	theme	GPs	614:616	arg1	co-application					596:609	the co-application	592:609	the co-application of GPs	592:616	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	3	66	theme	GPs	614:616	arg1	curcumin					622:629	curcumin	622:629	curcumin in the form of pharmaceutical composites	622:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	8	67	theme	cytokines	1437:1445	arg1	secretion					1447:1455	cytokines secretion	1437:1455	cytokines secretion	1437:1455	The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion.
31323374	3	68	from	benefits	580:587	arg1	form					638:641	the form	634:641	the form of pharmaceutical composites	634:670	The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites.
31323374	1	69	theme	immunomodulatory	238:253	arg1	potential					277:285	immunomodulatory and anti-inflammatory potential	238:285	immunomodulatory and anti-inflammatory potential both in vitro and in vivo	238:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	0	70	theme	anti-inflammatory	62:78	arg1	potential					80:88	its anti-inflammatory potential	58:88	its anti-inflammatory potential	58:88	Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro.
31323374	1	71	dep	in	305:306	arg1	vivo					308:311	vivo	308:311	vivo	308:311	Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo.
31323374	7	72	theme	anti-oxidative	1284:1297	arg1	effect					1299:1304	an anti-oxidative effect	1281:1304	an anti-oxidative effect	1281:1304	Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect.
31323374	9	73	theme	curcumin	1532:1539	arg1	incorporation					1515:1527	the incorporation	1511:1527	the incorporation of curcumin into GPs	1511:1548	The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
31141562	7	0	theme	amylopectin	1179:1189	arg1	contents					1191:1198	lower total starch, amylose and amylopectin contents	1147:1198	lower total starch, amylose and amylopectin contents	1147:1198	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	7	1	theme	water	1093:1097	arg1	homogenization					1099:1112	water homogenization	1093:1112	water homogenization	1093:1112	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	3	2	dep	compare	325:331	arg1	starches					509:516	starches	509:516	starches isolated using two different extraction methods	509:564	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	2	dep	compare	325:331	arg1	flour					499:503	grain flour	493:503	grain flour	493:503	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	0	3	dep	vulgare	109:115	arg1	L					117:117	L	117:117	Hordeum vulgare L.	101:118	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	6	4	theme	size	963:966	arg1	distribution					968:979	granule size distribution	955:979	granule size distribution	955:979	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	2	5	used	used	316:319	arg2	flour					300:304	the whole grain flour	284:304	the whole grain flour	284:304	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	8	6	theme	grain	1309:1313	arg1	flour					1315:1319	the whole grain flour	1299:1319	the whole grain flour	1299:1319	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	8	7	theme	pasting	1348:1354	arg1	properties					1356:1365	the pasting properties	1344:1365	the pasting properties in breeding programs	1344:1386	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	6	8	theme	granule	955:961	arg1	distribution					968:979	granule size distribution	955:979	granule size distribution	955:979	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	7	9	theme	amylose	1167:1173	arg1	contents					1191:1198	lower total starch, amylose and amylopectin contents	1147:1198	lower total starch, amylose and amylopectin contents	1147:1198	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	3	10	theme	extraction	547:556	arg1	methods					558:564	two different extraction methods	533:564	two different extraction methods	533:564	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	7	11	contain	had	1114:1116	arg2	contents					1191:1198	lower total starch, amylose and amylopectin contents	1147:1198	lower total starch, amylose and amylopectin contents	1147:1198	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	7	11	contain	had	1114:1116	arg1	starch					1072:1077	the starch	1068:1077	the starch isolated from water homogenization	1068:1112	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	7	11	contain	had	1114:1116	arg2	content					1135:1141	a higher protein content	1118:1141	a higher protein content	1118:1141	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	7	12	attach	isolated	1079:1086	arg2	starch					1072:1077	the starch	1068:1077	the starch isolated from water homogenization	1068:1112	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	7	12	attach	isolated	1079:1086	arg1	homogenization					1099:1112	water homogenization	1093:1112	water homogenization	1093:1112	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	3	13	used	used	402:405	arg2	we					399:400	we	399:400	we	399:400	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	14	theme	types	475:479	arg1	properties					455:464	pasting properties	447:464	pasting properties of three types of samples	447:490	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	0	15	dep	barley	93:98	arg1	vulgare					109:115	Hordeum vulgare	101:115	Hordeum vulgare L.	101:118	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	4	16	theme	starch	654:659	arg1	samples					661:667	the two starch samples	646:667	the two starch samples	646:667	We also investigated compositional, morphological and structural properties of the two starch samples.
31141562	7	17	theme	starch	1159:1164	arg1	contents					1191:1198	lower total starch, amylose and amylopectin contents	1147:1198	lower total starch, amylose and amylopectin contents	1147:1198	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	2	18	theme	grains	254:259	arg1	consuming					269:277	consuming	269:277	consuming	269:277	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	2	18	theme	grains	254:259	arg1	isolation					222:230	The isolation	218:230	The isolation of starch form barley grains	218:259	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	3	19	theme	whole	380:384	arg1	flour					392:396	the whole grain flour	376:396	the whole grain flour	376:396	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	4	20	theme	compositional	588:600	arg1	properties					632:641	compositional, morphological and structural properties	588:641	compositional, morphological and structural properties of the two starch samples	588:667	We also investigated compositional, morphological and structural properties of the two starch samples.
31141562	8	21	used	used	1328:1331	arg2	flour					1315:1319	the whole grain flour	1299:1319	the whole grain flour	1299:1319	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	8	22	theme	whole	1303:1307	arg1	flour					1315:1319	the whole grain flour	1299:1319	the whole grain flour	1299:1319	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	2	23	theme	barley	247:252	arg1	grains					254:259	starch form barley grains	235:259	starch form barley grains	235:259	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	1	24	theme	barley	144:149	arg1	starch					151:156	barley starch	144:156	barley starch	144:156	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	7	25	theme	lower	1147:1151	arg1	starch					1159:1164	lower total starch	1147:1164	lower total starch	1147:1164	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	6	26	theme	amylose/amylopectin	908:926	arg1	ratio					928:932	amylose/amylopectin ratio	908:932	amylose/amylopectin ratio	908:932	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	2	27	theme	whole	288:292	arg1	flour					300:304	the whole grain flour	284:304	the whole grain flour	284:304	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	1	28	theme	starch	151:156	arg1	characteristics					172:186	important characteristics	162:186	important characteristics	162:186	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	1	28	theme	starch	151:156	arg1	properties					130:139	Pasting properties	122:139	Pasting properties of barley starch	122:156	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	3	29	theme	starch	355:360	arg1	properties					341:350	pasting properties	333:350	pasting properties of starch	333:360	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	6	30	theme	granule	935:941	arg1	morphology					943:952	granule morphology	935:952	granule morphology	935:952	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	3	31	theme	grain	493:497	arg1	flour					499:503	grain flour	493:503	grain flour	493:503	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	32	theme	grain	386:390	arg1	flour					392:396	the whole grain flour	376:396	the whole grain flour	376:396	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	0	33	theme	properties	22:31	arg1	Comparison					0:9	Comparison	0:9	Comparison of pasting properties	0:31	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	8	34	theme	breeding	1370:1377	arg1	programs					1379:1386	breeding programs	1370:1386	breeding programs	1370:1386	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	1	35	theme	important	162:170	arg1	characteristics					172:186	important characteristics	162:186	important characteristics	162:186	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	1	35	theme	important	162:170	arg1	properties					130:139	Pasting properties	122:139	Pasting properties of barley starch	122:156	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	0	36	theme	pasting	14:20	arg1	properties					22:31	pasting properties	14:31	pasting properties	14:31	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	0	37	theme	Hordeum	101:107	arg1	vulgare					109:115	Hordeum vulgare	101:115	Hordeum vulgare L.	101:118	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	5	38	from	differences	682:692	arg1	properties					705:714	pasting properties	697:714	pasting properties	697:714	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	3	39	theme	samples	484:490	arg1	types					475:479	three types	469:479	three types of samples	469:490	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	40	theme	Rapid	409:413	arg1	RVA					431:433	RVA	431:433	RVA	431:433	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	40	theme	Rapid	409:413	arg1	Analyser					421:428	a Rapid Visco Analyser	407:428	a Rapid Visco Analyser (RVA)	407:434	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	7	41	theme	higher	1120:1125	arg1	content					1135:1141	a higher protein content	1118:1141	a higher protein content	1118:1141	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	4	42	theme	morphological	603:615	arg1	properties					632:641	compositional, morphological and structural properties	588:641	compositional, morphological and structural properties of the two starch samples	588:667	We also investigated compositional, morphological and structural properties of the two starch samples.
31141562	7	43	theme	total	1153:1157	arg1	starch					1159:1164	lower total starch	1147:1164	lower total starch	1147:1164	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	6	44	located	found	899:903	arg2	differences					882:892	No significant differences	867:892	No significant differences	867:892	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	6	44	located	found	899:903	arg1	morphology					943:952	granule morphology	935:952	granule morphology	935:952	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	6	44	located	found	899:903	arg1	ratio					928:932	amylose/amylopectin ratio	908:932	amylose/amylopectin ratio	908:932	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	6	44	located	found	899:903	arg1	distribution					968:979	granule size distribution	955:979	granule size distribution	955:979	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	6	44	located	found	899:903	arg1	structure					993:1001	crystal structure	985:1001	crystal structure	985:1001	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	5	45	theme	significant	816:826	arg1	correlations					828:839	significant correlations	816:839	significant correlations between flour and starch	816:864	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	5	46	theme	pasting	787:793	arg1	properties					795:804	pasting properties	787:804	pasting properties	787:804	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	7	47	theme	protein	1127:1133	arg1	content					1135:1141	a higher protein content	1118:1141	a higher protein content	1118:1141	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	3	48	theme	pasting	333:339	arg1	properties					341:350	pasting properties	333:350	pasting properties of starch	333:360	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	6	49	theme	different	1040:1048	arg1	methods					1050:1056	two different methods	1036:1056	two different methods	1036:1056	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	0	50	theme	whole	51:55	arg1	flour					63:67	the whole grain flour	47:67	the whole grain flour	47:67	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	7	51	theme	alkaline	1260:1267	arg1	conditions					1269:1278	alkaline conditions	1260:1278	alkaline conditions	1260:1278	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	5	52	theme	sample	743:748	arg1	types					750:754	the three sample types	733:754	the three sample types	733:754	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	6	53	theme	significant	870:880	arg1	differences					882:892	No significant differences	867:892	No significant differences	867:892	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	7	54	theme	homogenized	1231:1241	arg1	extraction					1243:1252	homogenized extraction	1231:1252	homogenized extraction	1231:1252	However, the starch isolated from water homogenization had a higher protein content and lower total starch, amylose and amylopectin contents than the starch extracted with homogenized extraction under alkaline conditions.
31141562	3	55	theme	different	537:545	arg1	methods					558:564	two different extraction methods	533:564	two different extraction methods	533:564	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	2	56	theme	form	242:245	arg1	grains					254:259	starch form barley grains	235:259	starch form barley grains	235:259	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	6	57	theme	crystal	985:991	arg1	structure					993:1001	crystal structure	985:1001	crystal structure	985:1001	No significant differences were found in amylose/amylopectin ratio, granule morphology, granule size distribution and crystal structure between starches extracted using two different methods.
31141562	3	58	theme	Visco	415:419	arg1	RVA					431:433	RVA	431:433	RVA	431:433	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	3	58	theme	Visco	415:419	arg1	Analyser					421:428	a Rapid Visco Analyser	407:428	a Rapid Visco Analyser (RVA)	407:434	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	1	59	theme	processing	195:204	arg1	standpoint					206:215	a processing standpoint	193:215	a processing standpoint	193:215	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	1	60	from	standpoint	206:215	arg1	characteristics					172:186	important characteristics	162:186	important characteristics	162:186	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	1	60	from	standpoint	206:215	arg1	properties					130:139	Pasting properties	122:139	Pasting properties of barley starch	122:156	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	2	61	theme	starch	235:240	arg1	grains					254:259	starch form barley grains	235:259	starch form barley grains	235:259	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	2	62	theme	grain	294:298	arg1	flour					300:304	the whole grain flour	284:304	the whole grain flour	284:304	The isolation of starch form barley grains is time consuming thus the whole grain flour is always used.
31141562	4	63	theme	samples	661:667	arg1	properties					632:641	compositional, morphological and structural properties	588:641	compositional, morphological and structural properties of the two starch samples	588:667	We also investigated compositional, morphological and structural properties of the two starch samples.
31141562	5	64	theme	pasting	697:703	arg1	properties					705:714	pasting properties	697:714	pasting properties	697:714	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	0	65	from	starch	83:88	arg1	barley					93:98	barley	93:98	barley (Hordeum vulgare L.)	93:119	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	4	66	theme	structural	621:630	arg1	properties					632:641	compositional, morphological and structural properties	588:641	compositional, morphological and structural properties of the two starch samples	588:667	We also investigated compositional, morphological and structural properties of the two starch samples.
31141562	5	67	theme	properties	795:804	arg1	parameters					773:782	the parameters	769:782	the parameters of pasting properties	769:804	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31141562	0	68	theme	grain	57:61	arg1	flour					63:67	the whole grain flour	47:67	the whole grain flour	47:67	Comparison of pasting properties measured from the whole grain flour and extracted starch in barley (Hordeum vulgare L.).
31141562	1	69	theme	Pasting	122:128	arg1	characteristics					172:186	important characteristics	162:186	important characteristics	162:186	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	1	69	theme	Pasting	122:128	arg1	properties					130:139	Pasting properties	122:139	Pasting properties of barley starch	122:156	Pasting properties of barley starch are important characteristics from a processing standpoint.
31141562	3	70	theme	pasting	447:453	arg1	properties					455:464	pasting properties	447:464	pasting properties of three types of samples	447:490	To compare pasting properties of starch with those of the whole grain flour, we used a Rapid Visco Analyser (RVA) to measure pasting properties of three types of samples: grain flour and starches isolated using two different extraction methods.
31141562	8	71	from	properties	1356:1365	arg1	programs					1379:1386	breeding programs	1370:1386	breeding programs	1370:1386	We concluded that the whole grain flour can be used to predict the pasting properties in breeding programs.
31141562	5	72	theme	Significant	670:680	arg1	differences					682:692	Significant differences	670:692	Significant differences in pasting properties	670:714	Significant differences in pasting properties were found among the three sample types, but most of the parameters of pasting properties displayed significant correlations between flour and starch.
31494303	1	0	theme	weight	151:156	arg1	38 kDa					158:163	molecular weight 38 kDa	141:163	molecular weight 38 kDa	141:163	From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38 kDa and pectic substances.
31494303	2	1	theme	2.6:1.0	299:305	arg1	ratio					290:294	a ratio	288:294	a ratio of 2.6:1.0 and β-galacturonic acid	288:329	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	5	2	theme	branched	725:732	arg1	rhamnogalacturonan					734:751	a branched rhamnogalacturonan	723:751	a branched rhamnogalacturonan	723:751	According to NMR data it contains also a branched rhamnogalacturonan.
31494303	3	3	theme	β-1,6-glycosidic	505:520	arg1	bonds					522:526	β-1,6-glycosidic bonds	505:526	β-1,6-glycosidic bonds as a main chain	505:542	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	4	theme	main	533:536	arg1	chain					538:542	a main chain	531:542	a main chain	531:542	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	5	theme	chemical	356:363	arg1	methods					365:371	chemical methods	356:371	chemical methods	356:371	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	1	6	theme	pectic	169:174	arg1	substances					176:185	pectic substances	169:185	pectic substances	169:185	From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38 kDa and pectic substances.
31494303	2	7	theme	acid	326:329	arg1	ratio					290:294	a ratio	288:294	a ratio of 2.6:1.0 and β-galacturonic acid	288:329	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	4	8	theme	small	669:673	arg1	amounts					675:681	small amounts	669:681	small amounts	669:681	Pectic substance was found in small amounts.
31494303	5	9	contain	contains	709:716	arg1	it					706:707	it	706:707	it	706:707	According to NMR data it contains also a branched rhamnogalacturonan.
31494303	5	9	contain	contains	709:716	arg2	rhamnogalacturonan					734:751	a branched rhamnogalacturonan	723:751	a branched rhamnogalacturonan	723:751	According to NMR data it contains also a branched rhamnogalacturonan.
31494303	2	10	theme	β-galacturonic	311:324	arg1	acid					326:329	β-galacturonic acid	311:329	β-galacturonic acid	311:329	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	4	11	theme	Pectic	639:644	arg1	substance					646:654	Pectic substance	639:654	Pectic substance	639:654	Pectic substance was found in small amounts.
31494303	0	12	theme	arabinogalactan	13:27	arg1	Structure					0:8	Structure	0:8	Structure of arabinogalactan and pectin from the Silybum marianum.	0:65	Structure of arabinogalactan and pectin from the Silybum marianum.
31494303	4	13	located	found	660:664	arg2	substance					646:654	Pectic substance	639:654	Pectic substance	639:654	Pectic substance was found in small amounts.
31494303	4	13	located	found	660:664	arg1	amounts					675:681	small amounts	669:681	small amounts	669:681	Pectic substance was found in small amounts.
31494303	3	14	theme	side	553:556	arg1	chain					558:562	the side chain	549:562	the side chain	549:562	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	15	theme	2D	395:396	arg1	spectroscopy					402:413	1D and 2D NMR spectroscopy	388:413	1D and 2D NMR spectroscopy	388:413	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	15	theme	2D	395:396	arg1	GC					377:378	GC	377:378	GC	377:378	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	0	16	theme	pectin	33:38	arg1	Structure					0:8	Structure	0:8	Structure of arabinogalactan and pectin from the Silybum marianum.	0:65	Structure of arabinogalactan and pectin from the Silybum marianum.
31494303	3	17	theme	NMR	398:400	arg1	spectroscopy					402:413	1D and 2D NMR spectroscopy	388:413	1D and 2D NMR spectroscopy	388:413	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	17	theme	NMR	398:400	arg1	GC					377:378	GC	377:378	GC	377:378	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	0	18	theme	Silybum	49:55	arg1	marianum					57:64	the Silybum marianum	45:64	the Silybum marianum	45:64	Structure of arabinogalactan and pectin from the Silybum marianum.
31494303	0	19	from	marianum	57:64	arg1	Structure					0:8	Structure	0:8	Structure of arabinogalactan and pectin from the Silybum marianum.	0:65	Structure of arabinogalactan and pectin from the Silybum marianum.
31494303	2	20	theme	arabinogalactan	222:236	arg1	component					342:350	a minor component	334:350	a minor component	334:350	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	2	20	theme	arabinogalactan	222:236	arg1	composition					207:217	The monosaccharide composition	188:217	The monosaccharide composition of arabinogalactan	188:236	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	1	21	dep	the	72:74	arg1	leaves					76:81	leaves	76:81	leaves	76:81	From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38 kDa and pectic substances.
31494303	3	22	theme	4-O-methylglucuronic	612:631	arg1	acid					633:636	4-O-methylglucuronic acid	612:636	4-O-methylglucuronic acid	612:636	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	2	23	theme	minor	336:340	arg1	component					342:350	a minor component	334:350	a minor component	334:350	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	2	23	theme	minor	336:340	arg1	composition					207:217	The monosaccharide composition	188:217	The monosaccharide composition of arabinogalactan	188:236	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	5	24	theme	NMR	697:699	arg1	data					701:704	NMR data	697:704	NMR data	697:704	According to NMR data it contains also a branched rhamnogalacturonan.
31494303	2	25	theme	monosaccharide	192:205	arg1	component					342:350	a minor component	334:350	a minor component	334:350	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	2	25	theme	monosaccharide	192:205	arg1	composition					207:217	The monosaccharide composition	188:217	The monosaccharide composition of arabinogalactan	188:236	The monosaccharide composition of arabinogalactan was represented by β-galactose and α-arabinose in a ratio of 2.6:1.0 and β-galacturonic acid as a minor component.
31494303	3	26	theme	d-galactopyranose	468:484	arg1	residues					486:493	d-galactopyranose residues	468:493	d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain	468:542	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	1	27	dep	Silybum	86:92	arg1	L.					103:104	Silybum marianum L.	86:104	Silybum marianum L.	86:104	From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38 kDa and pectic substances.
31494303	3	28	theme	1D	388:389	arg1	spectroscopy					402:413	1D and 2D NMR spectroscopy	388:413	1D and 2D NMR spectroscopy	388:413	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	3	28	theme	1D	388:389	arg1	GC					377:378	GC	377:378	GC	377:378	By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by β-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, β-galactose and 4-O-methylglucuronic acid.
31494303	1	29	theme	molecular	141:149	arg1	38 kDa					158:163	molecular weight 38 kDa	141:163	molecular weight 38 kDa	141:163	From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38 kDa and pectic substances.
29603538	4	0	theme	Wzx-/Wzy-dependent	947:964	arg1	pathway					966:972	the Wzx-/Wzy-dependent pathway	943:972	the Wzx-/Wzy-dependent pathway	943:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	3	1	theme	molecules	554:562	arg1	purification					534:545	purification	534:545	purification of OPS molecules of suboptimal or of mixed lengths	534:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	9	2	theme	STUDY	1813:1817	arg1	SIGNIFICANCE					1782:1793	SIGNIFICANCE	1782:1793	SIGNIFICANCE	1782:1793	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	9	2	theme	STUDY	1813:1817	arg1	IMPACT					1799:1804	IMPACT	1799:1804	IMPACT OF THE STUDY	1799:1817	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	0	3	theme	glycoconjugate	214:227	arg1	vaccines					229:236	glycoconjugate vaccines	214:236	glycoconjugate vaccines	214:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	3	4	theme	lengths	590:596	arg1	molecules					554:562	OPS molecules	550:562	OPS molecules of suboptimal or of mixed lengths	550:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	1	5	theme	several	305:311	arg1	pathogens					323:331	several bacterial pathogens	305:331	several bacterial pathogens	305:331	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	4	6	theme	length	922:927	arg1	regulators					929:938	O-polysaccharide chain length regulators	899:938	O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	899:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	5	7	from	wzz2	1089:1092	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	7	from	wzz2	1089:1092	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	7	from	wzz2	1089:1092	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	9	8	from	use	1975:1977	arg1	development					2026:2036	glycoconjugate vaccine development	2003:2036	glycoconjugate vaccine development	2003:2036	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	5	9	dep	Pseudomonas	1099:1109	arg1	aeruginosa					1111:1120	aeruginosa	1111:1120	aeruginosa	1111:1120	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	0	10	theme	defined	145:151	arg1	polymers					183:190	defined modal length O-polysaccharide polymers	145:190	defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines	145:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	3	11	theme	additional	609:618	arg1	costs					620:624	additional costs	609:624	additional costs	609:624	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	5	12	from	PAO1	1122:1125	arg1	wzz2					1089:1092	wzz2	1089:1092	wzz2	1089:1092	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	12	from	PAO1	1122:1125	arg1	I77					1081:1083	Salmonella Typhimurium I77	1058:1083	Salmonella Typhimurium I77	1058:1083	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	1	13	theme	AIMS	239:242	arg1	OPS					262:264	OPS	262:264	OPS	262:264	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	1	13	theme	AIMS	239:242	arg1	O-polysaccharide					244:259	AIMS O-polysaccharide	239:259	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	0	14	theme	length	159:164	arg1	polymers					183:190	defined modal length O-polysaccharide polymers	145:190	defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines	145:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	6	15	theme	chain	1340:1344	arg1	O-polysaccharides					1346:1362	long or very long chain O-polysaccharides	1322:1362	long or very long chain O-polysaccharides	1322:1362	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	4	16	theme	bacteria	766:773	arg1	utility					729:735	the utility	725:735	the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	725:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	0	17	theme	polymers	183:190	arg1	production					131:140	production	131:140	production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines	131:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	5	18	theme	chain	1015:1019	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	18	theme	chain	1015:1019	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	18	theme	chain	1015:1019	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	19	dep	Salmonella	1058:1067	arg1	Typhimurium					1069:1079	Typhimurium	1069:1079	Typhimurium	1069:1079	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	9	20	theme	OPS	1921:1923	arg1	haptens					1933:1939	preferred OPS vaccine haptens	1911:1939	preferred OPS vaccine haptens	1911:1939	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	6	21	theme	long	1322:1325	arg1	O-polysaccharides					1346:1362	long or very long chain O-polysaccharides	1322:1362	long or very long chain O-polysaccharides	1322:1362	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	4	22	theme	engineering	740:750	arg1	bacteria					766:773	engineering Gram-negative bacteria	740:773	engineering Gram-negative bacteria	740:773	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	1	23	theme	glycoconjugate	374:387	arg1	vaccines					389:396	glycoconjugate vaccines	374:396	glycoconjugate vaccines	374:396	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	8	24	theme	lipopolysaccharide	1647:1664	arg1	synthesis					1666:1674	lipopolysaccharide synthesis	1647:1674	lipopolysaccharide synthesis	1647:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	5	25	theme	Gram-negative	1192:1204	arg1	bacteria					1206:1213	other Gram-negative bacteria	1186:1213	other Gram-negative bacteria	1186:1213	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	2	26	theme	vaccines	488:495	arg1	development					467:477	further development	459:477	further development of these vaccines	459:495	Variability in the OPS chain length is one obstacle towards further development of these vaccines.
29603538	8	27	theme	genetic	1687:1693	arg1	method					1695:1700	a genetic method	1685:1700	a genetic method to increase the production of an O-polysaccharide population of a defined size	1685:1779	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	4	28	theme	O-polysaccharide	797:812	arg1	populations					814:824	homogenous O-polysaccharide populations	786:824	homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	786:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	4	28	theme	O-polysaccharide	797:812	arg1	basis					850:854	the basis	846:854	the basis of carbohydrate vaccines	846:879	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	2	29	theme	chain	422:426	arg1	length					428:433	the OPS chain length	414:433	the OPS chain length	414:433	Variability in the OPS chain length is one obstacle towards further development of these vaccines.
29603538	1	30	theme	bacterial	313:321	arg1	pathogens					323:331	several bacterial pathogens	305:331	several bacterial pathogens	305:331	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	5	31	dep	METHOD	975:980	arg1	expressed					1143:1151	expressed	1143:1151	expressed in the homologous organism or in other Gram-negative bacteria	1143:1213	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	31	dep	METHOD	975:980	arg1	cloned					1132:1137	cloned	1132:1137	cloned	1132:1137	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	2	32	theme	further	459:465	arg1	development					467:477	further development	459:477	further development of these vaccines	459:495	Variability in the OPS chain length is one obstacle towards further development of these vaccines.
29603538	0	33	theme	O-polysaccharide	18:33	arg1	regulators					48:57	O-polysaccharide chain length regulators	18:57	O-polysaccharide chain length regulators	18:57	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	7	34	theme	Paratyphi	1437:1445	arg1	A					1447:1447	Salmonella Paratyphi A	1426:1447	Salmonella Paratyphi A	1426:1447	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	0	35	theme	length	41:46	arg1	regulators					48:57	O-polysaccharide chain length regulators	18:57	O-polysaccharide chain length regulators	18:57	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	9	36	theme	vaccine	2018:2024	arg1	development					2026:2036	glycoconjugate vaccine development	2003:2036	glycoconjugate vaccine development	2003:2036	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	4	37	theme	O-polysaccharide	899:914	arg1	regulators					929:938	O-polysaccharide chain length regulators	899:938	O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	899:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	8	38	theme	O-polysaccharide	1735:1750	arg1	population					1752:1761	an O-polysaccharide population	1732:1761	an O-polysaccharide population of a defined size	1732:1779	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	8	39	theme	CONCLUSIONS	1538:1548	arg1	Overexpression					1550:1563	CONCLUSIONS Overexpression	1538:1563	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis	1538:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	3	40	theme	steps	521:525	arg1	Introduction					498:509	Introduction	498:509	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths	498:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	4	41	theme	carbohydrate	859:870	arg1	vaccines					872:879	carbohydrate vaccines	859:879	carbohydrate vaccines	859:879	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	8	42	theme	defined	1768:1774	arg1	size					1776:1779	a defined size	1766:1779	a defined size	1766:1779	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	3	43	theme	OPS	550:552	arg1	molecules					554:562	OPS molecules	550:562	OPS molecules of suboptimal or of mixed lengths	550:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	1	44	theme	protective	281:290	arg1	molecules					267:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	1	44	theme	protective	281:290	arg1	antigens					292:299	protective antigens	281:299	protective antigens for several bacterial pathogens	281:331	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	7	45	theme	same	1369:1372	arg1	observation					1374:1384	The same observation	1365:1384	The same observation	1365:1384	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	0	46	from	use	196:198	arg1	vaccines					229:236	glycoconjugate vaccines	214:236	glycoconjugate vaccines	214:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	3	47	theme	mixed	584:588	arg1	lengths					590:596	mixed lengths	584:596	mixed lengths	584:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	4	48	theme	pathway	966:972	arg1	regulators					929:938	O-polysaccharide chain length regulators	899:938	O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	899:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	4	49	theme	chain	916:920	arg1	regulators					929:938	O-polysaccharide chain length regulators	899:938	O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	899:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	2	50	from	Variability	399:409	arg1	length					428:433	the OPS chain length	414:433	the OPS chain length	414:433	Variability in the OPS chain length is one obstacle towards further development of these vaccines.
29603538	4	51	theme	study	700:704	arg1	goal					687:690	The overall goal	675:690	The overall goal of this study	675:704	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	7	52	theme	aeruginosa	1526:1535	arg1	strains					1512:1518	two other strains	1502:1518	two other strains of P. aeruginosa	1502:1535	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	0	53	theme	modal	153:157	arg1	polymers					183:190	defined modal length O-polysaccharide polymers	145:190	defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines	145:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	8	54	from	Overexpression	1550:1563	arg1	bacteria					1598:1605	Gram-negative bacteria	1584:1605	Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis	1584:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	6	55	theme	homologous	1260:1269	arg1	organism					1271:1278	the homologous organism	1256:1278	the homologous organism	1256:1278	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	9	56	theme	cost-effective	1860:1873	arg1	strategy					1888:1895	a cost-effective and improved strategy	1858:1895	a cost-effective and improved strategy for isolating preferred OPS vaccine haptens	1858:1939	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	8	57	theme	proteins	1572:1579	arg1	Overexpression					1550:1563	CONCLUSIONS Overexpression	1538:1563	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis	1538:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	4	58	theme	overall	679:685	arg1	goal					687:690	The overall goal	675:690	The overall goal of this study	675:704	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	0	59	theme	O-polysaccharide	166:181	arg1	polymers					183:190	defined modal length O-polysaccharide polymers	145:190	defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines	145:236	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	1	60	theme	O-polysaccharide	244:259	arg1	molecules					267:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	1	60	theme	O-polysaccharide	244:259	arg1	antigens					292:299	protective antigens	281:299	protective antigens for several bacterial pathogens	281:331	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	9	61	theme	improved	1879:1886	arg1	strategy					1888:1895	a cost-effective and improved strategy	1858:1895	a cost-effective and improved strategy for isolating preferred OPS vaccine haptens	1858:1939	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	8	62	theme	Gram-negative	1584:1596	arg1	bacteria					1598:1605	Gram-negative bacteria	1584:1605	Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis	1584:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	6	63	theme	O-polysaccharides	1346:1362	arg1	proportion					1308:1317	the proportion	1304:1317	the proportion of long or very long chain O-polysaccharides	1304:1362	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	9	64	dep	SIGNIFICANCE	1782:1793	arg1	methods					1823:1829	The methods	1819:1829	The methods presented herein	1819:1846	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	5	65	theme	other	1186:1190	arg1	bacteria					1206:1213	other Gram-negative bacteria	1186:1213	other Gram-negative bacteria	1186:1213	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	66	theme	O-polysaccharide	998:1013	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	66	theme	O-polysaccharide	998:1013	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	66	theme	O-polysaccharide	998:1013	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	0	67	from	Overexpression	0:13	arg1	bacteria					76:83	Gram-negative bacteria	62:83	Gram-negative bacteria using the Wzx-/Wzy-dependent pathway	62:120	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	6	68	theme	long	1335:1338	arg1	O-polysaccharides					1346:1362	long or very long chain O-polysaccharides	1322:1362	long or very long chain O-polysaccharides	1322:1362	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	4	69	theme	Gram-negative	752:764	arg1	bacteria					766:773	engineering Gram-negative bacteria	740:773	engineering Gram-negative bacteria	740:773	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	5	70	theme	length	1021:1026	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	70	theme	length	1021:1026	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	70	theme	length	1021:1026	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	9	71	theme	preferred	1911:1919	arg1	haptens					1933:1939	preferred OPS vaccine haptens	1911:1939	preferred OPS vaccine haptens	1911:1939	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	8	72	theme	Wzx/Wzy-dependant	1617:1633	arg1	pathway					1635:1641	the Wzx/Wzy-dependant pathway	1613:1641	the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis	1613:1674	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	5	73	theme	homologous	1160:1169	arg1	organism					1171:1178	the homologous organism	1156:1178	the homologous organism	1156:1178	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	3	74	theme	final	662:666	arg1	yield					668:672	the final yield	658:672	the final yield	658:672	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	9	75	theme	vaccine	1925:1931	arg1	haptens					1933:1939	preferred OPS vaccine haptens	1911:1939	preferred OPS vaccine haptens	1911:1939	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	0	76	theme	Wzx-/Wzy-dependent	95:112	arg1	pathway					114:120	the Wzx-/Wzy-dependent pathway	91:120	the Wzx-/Wzy-dependent pathway	91:120	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	9	77	theme	further	1967:1973	arg1	use					1975:1977	the further use	1963:1977	the further use of O-polysaccharides in glycoconjugate vaccine development	1963:2036	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	1	78	theme	vaccines	389:396	arg1	components					360:369	components	360:369	components of glycoconjugate vaccines	360:396	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	5	79	theme	Salmonella	1058:1067	arg1	I77					1081:1083	Salmonella Typhimurium I77	1058:1083	Salmonella Typhimurium I77	1058:1083	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	1	80	contain	have	338:341	arg1	molecules					267:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules	239:275	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	1	80	contain	have	338:341	arg1	antigens					292:299	protective antigens	281:299	protective antigens for several bacterial pathogens	281:331	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	1	80	contain	have	338:341	arg2	utility					349:355	broad utility	343:355	broad utility as components of glycoconjugate vaccines	343:396	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	5	81	dep	regulators	1028:1037	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	81	dep	regulators	1028:1037	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	81	dep	regulators	1028:1037	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	7	82	theme	Shigella	1453:1460	arg1	flexneri					1462:1469	Shigella flexneri	1453:1469	Shigella flexneri	1453:1469	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	4	83	theme	homogenous	786:795	arg1	populations					814:824	homogenous O-polysaccharide populations	786:824	homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	786:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	4	83	theme	homogenous	786:795	arg1	basis					850:854	the basis	846:854	the basis of carbohydrate vaccines	846:879	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	2	84	theme	OPS	418:420	arg1	length					428:433	the OPS chain length	414:433	the OPS chain length	414:433	Variability in the OPS chain length is one obstacle towards further development of these vaccines.
29603538	0	85	theme	chain	35:39	arg1	regulators					48:57	O-polysaccharide chain length regulators	18:57	O-polysaccharide chain length regulators	18:57	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	7	86	theme	Salmonella	1426:1435	arg1	A					1447:1447	Salmonella Paratyphi A	1426:1447	Salmonella Paratyphi A	1426:1447	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	9	87	theme	O-polysaccharides	1982:1998	arg1	use					1975:1977	the further use	1963:1977	the further use of O-polysaccharides in glycoconjugate vaccine development	1963:2036	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	3	88	theme	sizing	514:519	arg1	steps					521:525	sizing steps	514:525	sizing steps	514:525	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	0	89	theme	regulators	48:57	arg1	Overexpression					0:13	Overexpression	0:13	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway	0:120	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	9	90	theme	glycoconjugate	2003:2016	arg1	development					2026:2036	glycoconjugate vaccine development	2003:2036	glycoconjugate vaccine development	2003:2036	SIGNIFICANCE AND IMPACT OF THE STUDY The methods presented herein represent a cost-effective and improved strategy for isolating preferred OPS vaccine haptens, and could facilitate the further use of O-polysaccharides in glycoconjugate vaccine development.
29603538	4	91	theme	vaccines	872:879	arg1	populations					814:824	homogenous O-polysaccharide populations	786:824	homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	786:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	4	91	theme	vaccines	872:879	arg1	basis					850:854	the basis	846:854	the basis of carbohydrate vaccines	846:879	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	0	92	theme	Gram-negative	62:74	arg1	bacteria					76:83	Gram-negative bacteria	62:83	Gram-negative bacteria using the Wzx-/Wzy-dependent pathway	62:120	Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines.
29603538	1	93	theme	broad	343:347	arg1	utility					349:355	broad utility	343:355	broad utility as components of glycoconjugate vaccines	343:396	AIMS O-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines.
29603538	6	94	theme	proteins	1244:1251	arg1	Overexpression					1216:1229	Overexpression	1216:1229	Overexpression of these Wzz proteins in the homologous organism	1216:1278	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	8	95	theme	population	1752:1761	arg1	production					1718:1727	the production	1714:1727	the production of an O-polysaccharide population of a defined size	1714:1779	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	4	96	used	used	838:841	arg2	basis					850:854	the basis	846:854	the basis of carbohydrate vaccines	846:879	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	4	96	used	used	838:841	arg2	populations					814:824	homogenous O-polysaccharide populations	786:824	homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway	786:972	The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.
29603538	7	97	theme	other	1506:1510	arg1	strains					1512:1518	two other strains	1502:1518	two other strains of P. aeruginosa	1502:1535	The same observation was made when wzzB was overexpressed in Salmonella Paratyphi A and Shigella flexneri, and wzz2 was overexpressed in two other strains of P. aeruginosa.
29603538	5	98	theme	Pseudomonas	1099:1109	arg1	PAO1					1122:1125	Pseudomonas aeruginosa PAO1	1099:1125	Pseudomonas aeruginosa PAO1	1099:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	8	99	theme	size	1776:1779	arg1	population					1752:1761	an O-polysaccharide population	1732:1761	an O-polysaccharide population of a defined size	1732:1779	CONCLUSIONS Overexpression of Wzz proteins in Gram-negative bacteria using the Wzx/Wzy-dependant pathway for lipopolysaccharide synthesis provides a genetic method to increase the production of an O-polysaccharide population of a defined size.
29603538	6	100	from	Overexpression	1216:1229	arg1	organism					1271:1278	the homologous organism	1256:1278	the homologous organism	1256:1278	Overexpression of these Wzz proteins in the homologous organism significantly increased the proportion of long or very long chain O-polysaccharides.
29603538	3	101	theme	suboptimal	567:576	arg1	molecules					554:562	OPS molecules	550:562	OPS molecules of suboptimal or of mixed lengths	550:596	Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield.
29603538	5	102	from	I77	1081:1083	arg1	fepE					1048:1051	fepE	1048:1051	fepE	1048:1051	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	102	from	I77	1081:1083	arg1	wzzB					1039:1042	wzzB	1039:1042	wzzB	1039:1042	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29603538	5	102	from	I77	1081:1083	arg1	regulators					1028:1037	The O-polysaccharide chain length regulators	994:1037	The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1	994:1125	METHOD AND RESULTS The O-polysaccharide chain length regulators wzzB and fepE from Salmonella Typhimurium I77 and wzz2 from Pseudomonas aeruginosa PAO1 were cloned and expressed in the homologous organism or in other Gram-negative bacteria.
29459243	10	0	theme	citrate	1002:1008	arg1	citrate					1002:1008	citrate	1002:1008	citrate	1002:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	10	0	theme	citrate	1002:1008	arg1	amount					992:997	a small amount	984:997	a small amount of citrate	984:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	14	1	dep	resulted	1459:1466	arg1	obtained					1580:1587	obtained	1580:1587	obtained using a commercial culture	1580:1614	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	2	theme	ethanol	1515:1521	arg1	acetoin					1550:1556	acetoin	1550:1556	acetoin	1550:1556	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	2	theme	ethanol	1515:1521	arg1	diacetyl					1537:1544	diacetyl	1537:1544	diacetyl	1537:1544	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	2	theme	ethanol	1515:1521	arg1	ethanol					1515:1521	ethanol	1515:1521	ethanol	1515:1521	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	2	theme	ethanol	1515:1521	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	2	theme	ethanol	1515:1521	arg1	amounts					1504:1510	amounts	1504:1510	amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls	1504:1577	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	3	3	from	37 °C	368:372	arg1	production					382:391	flavor production	375:391	flavor production	375:391	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	3	from	37 °C	368:372	arg1	production					456:465	exopolysaccharide (EPS) production	432:465	exopolysaccharide (EPS) production	432:465	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	3	from	37 °C	368:372	arg1	growth					342:347	growth	342:347	growth at 20, 25, 30, and 37 °C	342:372	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	3	from	37 °C	368:372	arg1	folate					421:426	folate	421:426	folate	421:426	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	3	from	37 °C	368:372	arg1	AO					407:408	AO	407:408	AO	407:408	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	3	from	37 °C	368:372	arg1	activity					411:418	antioxidant (AO) activity	394:418	antioxidant (AO) activity	394:418	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	1	4	theme	Lactococcus	160:170	arg1	hircilactis					144:154	Lactococcus hircilactis	132:154	Lactococcus hircilactis	132:154	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	4	theme	Lactococcus	160:170	arg1	laudensis					172:180	Lactococcus laudensis	160:180	Lactococcus laudensis	160:180	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	4	theme	Lactococcus	160:170	arg1	cultures					205:212	starter cultures	197:212	starter cultures	197:212	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	4	5	theme	growth	502:507	arg1	temperature					509:519	the best growth temperature	493:519	the best growth temperature for both strains	493:536	At 30 °C, which resulted the best growth temperature for both strains, Lc.
29459243	11	6	theme	high	1134:1137	arg1	activity					1142:1149	high AO activity	1134:1149	high AO activity	1134:1149	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	14	7	theme	starter	1398:1404	arg1	lactococci					1372:1381	the two lactococci	1364:1381	the two lactococci	1364:1381	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	7	theme	starter	1398:1404	arg1	cultures					1406:1413	starter cultures	1398:1413	starter cultures in small-scale cheesemaking trials	1398:1448	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	2	8	theme	technological	280:292	arg1	interest					309:316	technological and functional interest	280:316	technological and functional interest	280:316	To this end, the two lactococci were characterized for traits of technological and functional interest.
29459243	11	9	theme	AO	1139:1140	arg1	activity					1142:1149	high AO activity	1134:1149	high AO activity	1134:1149	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	7	10	theme	lactate	753:759	arg1	accumulation					761:772	a higher lactate accumulation	744:772	a higher lactate accumulation	744:772	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	17	11	from	laudensis	1660:1668	arg1	cheesemaking					1694:1705	cheesemaking	1694:1705	cheesemaking	1694:1705	laudensis as aromatic cultures in cheesemaking is proposed.
29459243	3	12	from	25	356:357	arg1	production					382:391	flavor production	375:391	flavor production	375:391	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	12	from	25	356:357	arg1	production					456:465	exopolysaccharide (EPS) production	432:465	exopolysaccharide (EPS) production	432:465	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	12	from	25	356:357	arg1	growth					342:347	growth	342:347	growth at 20, 25, 30, and 37 °C	342:372	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	12	from	25	356:357	arg1	folate					421:426	folate	421:426	folate	421:426	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	12	from	25	356:357	arg1	AO					407:408	AO	407:408	AO	407:408	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	12	from	25	356:357	arg1	activity					411:418	antioxidant (AO) activity	394:418	antioxidant (AO) activity	394:418	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	11	13	theme	small	1165:1169	arg1	~30 mg/L					1187:1194	~30 mg/L	1187:1194	~30 mg/L	1187:1194	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	11	13	theme	small	1165:1169	arg1	EPS					1182:1184	EPS	1182:1184	EPS (~30 mg/L)	1182:1195	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	11	13	theme	small	1165:1169	arg1	amounts					1171:1177	small amounts	1165:1177	small amounts of EPS (~30 mg/L)	1165:1195	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	14	14	from	cultures	1406:1413	arg1	trials					1443:1448	small-scale cheesemaking trials	1418:1448	small-scale cheesemaking trials	1418:1448	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	13	15	theme	uninoculated	1340:1351	arg1	milk					1353:1356	uninoculated milk	1340:1356	uninoculated milk	1340:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	3	16	theme	antioxidant	394:404	arg1	AO					407:408	AO	407:408	AO	407:408	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	16	theme	antioxidant	394:404	arg1	activity					411:418	antioxidant (AO) activity	394:418	antioxidant (AO) activity	394:418	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	production					382:391	flavor production	375:391	flavor production	375:391	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	production					456:465	exopolysaccharide (EPS) production	432:465	exopolysaccharide (EPS) production	432:465	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	growth					342:347	growth	342:347	growth at 20, 25, 30, and 37 °C	342:372	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	folate					421:426	folate	421:426	folate	421:426	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	AO					407:408	AO	407:408	AO	407:408	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	17	from	30	360:361	arg1	activity					411:418	antioxidant (AO) activity	394:418	antioxidant (AO) activity	394:418	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	13	18	from	levels	1310:1315	arg1	folate					1300:1305	folate at levels	1300:1315	folate at levels four times higher than uninoculated milk	1300:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	13	18	from	levels	1310:1315	arg1	at					1307:1308	folate at levels	1300:1315	folate at levels four times higher than uninoculated milk	1300:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	2	19	theme	interest	309:316	arg1	traits					270:275	traits	270:275	traits of technological and functional interest	270:316	To this end, the two lactococci were characterized for traits of technological and functional interest.
29459243	7	20	theme	higher	746:751	arg1	accumulation					761:772	a higher lactate accumulation	744:772	a higher lactate accumulation	744:772	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	3	21	theme	flavor	375:380	arg1	production					382:391	flavor production	375:391	flavor production	375:391	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	7	22	theme	higher	703:708	arg1	utilization					718:728	a higher lactose utilization	701:728	a higher lactose utilization	701:728	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	10	23	used	used	1014:1017	arg2	amount					992:997	a small amount	984:997	a small amount of citrate	984:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	10	23	used	used	1014:1017	arg2	citrate					1002:1008	citrate	1002:1008	citrate	1002:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	2	24	theme	functional	298:307	arg1	interest					309:316	technological and functional interest	280:316	technological and functional interest	280:316	To this end, the two lactococci were characterized for traits of technological and functional interest.
29459243	0	25	theme	hircilactis	29:39	arg1	Applicability					0:12	Applicability	0:12	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.	0:84	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.
29459243	7	26	theme	lactose	710:716	arg1	utilization					718:728	a higher lactose utilization	701:728	a higher lactose utilization	701:728	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	12	27	theme	Lactococcus	1198:1208	arg1	laudensis					1210:1218	Lactococcus laudensis	1198:1218	Lactococcus laudensis	1198:1218	Lactococcus laudensis showed a weak proteolytic activity while Lc.
29459243	12	28	theme	proteolytic	1234:1244	arg1	activity					1246:1253	a weak proteolytic activity	1227:1253	a weak proteolytic activity while Lc	1227:1262	Lactococcus laudensis showed a weak proteolytic activity while Lc.
29459243	6	29	theme	milk	595:598	arg1	pH					585:586	the pH	581:586	the pH of the milk to 4.8 and 5.5	581:613	laudensis lowered the pH of the milk to 4.8 and 5.5, respectively, after 24 h of incubation.
29459243	0	30	theme	Lactococcus	17:27	arg1	hircilactis					29:39	Lactococcus hircilactis	17:39	Lactococcus hircilactis	17:39	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.
29459243	4	31	theme	best	497:500	arg1	temperature					509:519	the best growth temperature	493:519	the best growth temperature for both strains	493:536	At 30 °C, which resulted the best growth temperature for both strains, Lc.
29459243	11	32	theme	different	1098:1106	arg1	production					1115:1124	a different flavor production	1096:1124	a different flavor production	1096:1124	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	9	33	contain	containing	924:933	arg1	medium					917:922	a selective medium	905:922	a selective medium containing citrate as the sole carbon source	905:967	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	9	33	contain	containing	924:933	arg2	citrate					935:941	citrate	935:941	citrate	935:941	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	9	33	contain	containing	924:933	arg2	source					962:967	the sole carbon source	946:967	the sole carbon source	946:967	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	1	34	theme	study	102:106	arg1	aim					90:92	The aim	86:92	The aim of this study	86:106	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	35	theme	starter	197:203	arg1	hircilactis					144:154	Lactococcus hircilactis	132:154	Lactococcus hircilactis	132:154	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	35	theme	starter	197:203	arg1	laudensis					172:180	Lactococcus laudensis	160:180	Lactococcus laudensis	160:180	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	35	theme	starter	197:203	arg1	cultures					205:212	starter cultures	197:212	starter cultures	197:212	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	0	36	theme	Lactococcus	45:55	arg1	laudensis					57:65	Lactococcus laudensis	45:65	Lactococcus laudensis	45:65	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.
29459243	13	37	theme	folate	1300:1305	arg1	levels					1310:1315	folate at levels	1300:1315	folate at levels four times higher than uninoculated milk	1300:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	14	38	theme	higher	1558:1563	arg1	acetoin					1550:1556	acetoin	1550:1556	acetoin	1550:1556	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	38	theme	higher	1558:1563	arg1	diacetyl					1537:1544	diacetyl	1537:1544	diacetyl	1537:1544	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	38	theme	higher	1558:1563	arg1	ethanol					1515:1521	ethanol	1515:1521	ethanol	1515:1521	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	38	theme	higher	1558:1563	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	38	theme	higher	1558:1563	arg1	amounts					1504:1510	amounts	1504:1510	amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls	1504:1577	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	13	39	theme	higher	1328:1333	arg1	levels					1310:1315	folate at levels	1300:1315	folate at levels four times higher than uninoculated milk	1300:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	7	40	theme	acid	674:677	arg1	composition					679:689	organic acid composition	666:689	organic acid composition	666:689	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	14	41	theme	acetic	1524:1529	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	42	theme	acetoin	1550:1556	arg1	acetoin					1550:1556	acetoin	1550:1556	acetoin	1550:1556	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	42	theme	acetoin	1550:1556	arg1	diacetyl					1537:1544	diacetyl	1537:1544	diacetyl	1537:1544	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	42	theme	acetoin	1550:1556	arg1	ethanol					1515:1521	ethanol	1515:1521	ethanol	1515:1521	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	42	theme	acetoin	1550:1556	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	42	theme	acetoin	1550:1556	arg1	amounts					1504:1510	amounts	1504:1510	amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls	1504:1577	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	11	43	theme	EPS	1182:1184	arg1	~30 mg/L					1187:1194	~30 mg/L	1187:1194	~30 mg/L	1187:1194	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	11	43	theme	EPS	1182:1184	arg1	EPS					1182:1184	EPS	1182:1184	EPS (~30 mg/L)	1182:1195	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	11	43	theme	EPS	1182:1184	arg1	amounts					1171:1177	small amounts	1165:1177	small amounts of EPS (~30 mg/L)	1165:1195	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	3	44	from	20	352:353	arg1	production					382:391	flavor production	375:391	flavor production	375:391	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	44	from	20	352:353	arg1	production					456:465	exopolysaccharide (EPS) production	432:465	exopolysaccharide (EPS) production	432:465	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	44	from	20	352:353	arg1	growth					342:347	growth	342:347	growth at 20, 25, 30, and 37 °C	342:372	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	44	from	20	352:353	arg1	folate					421:426	folate	421:426	folate	421:426	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	44	from	20	352:353	arg1	AO					407:408	AO	407:408	AO	407:408	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	3	44	from	20	352:353	arg1	activity					411:418	antioxidant (AO) activity	394:418	antioxidant (AO) activity	394:418	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	0	45	theme	laudensis	57:65	arg1	Applicability					0:12	Applicability	0:12	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.	0:84	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.
29459243	14	46	theme	diacetyl	1537:1544	arg1	acetoin					1550:1556	acetoin	1550:1556	acetoin	1550:1556	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	46	theme	diacetyl	1537:1544	arg1	diacetyl					1537:1544	diacetyl	1537:1544	diacetyl	1537:1544	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	46	theme	diacetyl	1537:1544	arg1	ethanol					1515:1521	ethanol	1515:1521	ethanol	1515:1521	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	46	theme	diacetyl	1537:1544	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	46	theme	diacetyl	1537:1544	arg1	amounts					1504:1510	amounts	1504:1510	amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls	1504:1577	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	4	47	dep	Lc	539:540	arg1	30 °C					471:475	30 °C	471:475	30 °C	471:475	At 30 °C, which resulted the best growth temperature for both strains, Lc.
29459243	6	48	theme	incubation	644:653	arg1	24 h					636:639	24 h	636:639	24 h of incubation	636:653	laudensis lowered the pH of the milk to 4.8 and 5.5, respectively, after 24 h of incubation.
29459243	3	49	theme	exopolysaccharide	432:448	arg1	production					456:465	exopolysaccharide (EPS) production	432:465	exopolysaccharide (EPS) production	432:465	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	7	50	theme	organic	666:672	arg1	composition					679:689	organic acid composition	666:689	organic acid composition	666:689	Sugar and organic acid composition indicated a higher lactose utilization, coupled with a higher lactate accumulation, by Lc.
29459243	11	51	theme	flavor	1108:1113	arg1	production					1115:1124	a different flavor production	1096:1124	a different flavor production	1096:1124	The two strains were characterized by a different flavor production, showed high AO activity, and produced small amounts of EPS (~30 mg/L).
29459243	3	52	from	Tests	319:323	arg1	milk					328:331	milk	328:331	milk	328:331	Tests in milk included growth at 20, 25, 30, and 37 °C, flavor production, antioxidant (AO) activity, folate and exopolysaccharide (EPS) production.
29459243	1	53	used	used	189:192	arg2	laudensis					172:180	Lactococcus laudensis	160:180	Lactococcus laudensis	160:180	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	53	used	used	189:192	arg2	hircilactis					144:154	Lactococcus hircilactis	132:154	Lactococcus hircilactis	132:154	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	53	used	used	189:192	arg2	cultures					205:212	starter cultures	197:212	starter cultures	197:212	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	10	54	theme	small	986:990	arg1	citrate					1002:1008	citrate	1002:1008	citrate	1002:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	10	54	theme	small	986:990	arg1	amount					992:997	a small amount	984:997	a small amount of citrate	984:1008	Nevertheless, a small amount of citrate was used by both lactococci when grown in milk.
29459243	9	55	theme	selective	907:915	arg1	medium					917:922	a selective medium	905:922	a selective medium containing citrate as the sole carbon source	905:967	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	12	56	theme	weak	1229:1232	arg1	activity					1246:1253	a weak proteolytic activity	1227:1253	a weak proteolytic activity while Lc	1227:1262	Lactococcus laudensis showed a weak proteolytic activity while Lc.
29459243	14	57	theme	acid	1531:1534	arg1	acetoin					1550:1556	acetoin	1550:1556	acetoin	1550:1556	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	57	theme	acid	1531:1534	arg1	diacetyl					1537:1544	diacetyl	1537:1544	diacetyl	1537:1544	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	57	theme	acid	1531:1534	arg1	ethanol					1515:1521	ethanol	1515:1521	ethanol	1515:1521	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	57	theme	acid	1531:1534	arg1	acid					1531:1534	acetic acid	1524:1534	acetic acid	1524:1534	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	14	57	theme	acid	1531:1534	arg1	amounts					1504:1510	amounts	1504:1510	amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls	1504:1577	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	17	58	theme	aromatic	1673:1680	arg1	cultures					1682:1689	aromatic cultures	1673:1689	aromatic cultures	1673:1689	laudensis as aromatic cultures in cheesemaking is proposed.
29459243	15	59	theme	Lc	1636:1637	arg1	application					1621:1631	The application	1617:1631	The application of Lc.	1617:1638	The application of Lc.
29459243	13	60	theme	at	1307:1308	arg1	levels					1310:1315	folate at levels	1300:1315	folate at levels four times higher than uninoculated milk	1300:1356	hircilactis was able to accumulate folate at levels four times higher than uninoculated milk.
29459243	9	61	theme	sole	950:953	arg1	source					962:967	the sole carbon source	946:967	the sole carbon source	946:967	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	9	61	theme	sole	950:953	arg1	citrate					935:941	citrate	935:941	citrate	935:941	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	14	62	theme	cheesemaking	1430:1441	arg1	trials					1443:1448	small-scale cheesemaking trials	1418:1448	small-scale cheesemaking trials	1418:1448	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	1	63	theme	Lactococcus	132:142	arg1	hircilactis					144:154	Lactococcus hircilactis	132:154	Lactococcus hircilactis	132:154	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	63	theme	Lactococcus	132:142	arg1	laudensis					172:180	Lactococcus laudensis	160:180	Lactococcus laudensis	160:180	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	1	63	theme	Lactococcus	132:142	arg1	cultures					205:212	starter cultures	197:212	starter cultures	197:212	The aim of this study was to evaluate whether Lactococcus hircilactis and Lactococcus laudensis can be used as starter cultures.
29459243	14	64	theme	commercial	1597:1606	arg1	culture					1608:1614	a commercial culture	1595:1614	a commercial culture	1595:1614	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	0	65	theme	dairy	70:74	arg1	cultures					76:83	dairy cultures	70:83	dairy cultures	70:83	Applicability of Lactococcus hircilactis and Lactococcus laudensis as dairy cultures.
29459243	9	66	theme	Cit-	874:877	arg1	phenotype					879:887	a Cit- phenotype	872:887	a Cit- phenotype	872:887	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	14	67	theme	small-scale	1418:1428	arg1	trials					1443:1448	small-scale cheesemaking trials	1418:1448	small-scale cheesemaking trials	1418:1448	When the two lactococci were tested as starter cultures in small-scale cheesemaking trials, cheeses resulted of satisfying quality and contained amounts of ethanol, acetic acid, diacetyl and acetoin higher than controls, obtained using a commercial culture.
29459243	9	68	theme	carbon	955:960	arg1	source					962:967	the sole carbon source	946:967	the sole carbon source	946:967	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
29459243	9	68	theme	carbon	955:960	arg1	citrate					935:941	citrate	935:941	citrate	935:941	Both strains showed a Cit- phenotype after growth in a selective medium containing citrate as the sole carbon source.
31221327	6	0	theme	automotive	1061:1070	arg1	applications					1085:1096	automotive and building applications	1061:1096	automotive and building applications	1061:1096	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	1	1	theme	grass	145:149	arg1	binata					163:168	a fast growing perennial grass Eulaliopsis binata	120:168	a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai)	120:194	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	6	2	theme	thermal	998:1004	arg1	properties					1017:1026	thermal resistance properties	998:1026	thermal resistance properties	998:1026	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	1	3	theme	Eulaliopsis	151:161	arg1	binata					163:168	a fast growing perennial grass Eulaliopsis binata	120:168	a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai)	120:194	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	4	4	theme	Fiber	655:659	arg1	bundles					661:667	Fiber bundles	655:667	Fiber bundles extracted from the grass	655:692	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	4	5	theme	modulus	738:744	arg1	strength					706:713	tensile strength	698:713	tensile strength of 493 MPa and tensile modulus of 21 GPa	698:754	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	4	6	theme	493 MPa	718:724	arg1	strength					706:713	tensile strength	698:713	tensile strength of 493 MPa and tensile modulus of 21 GPa	698:754	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	4	7	contain	had	694:696	arg2	strength					706:713	tensile strength	698:713	tensile strength of 493 MPa and tensile modulus of 21 GPa	698:754	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	4	7	contain	had	694:696	arg1	bundles					661:667	Fiber bundles	655:667	Fiber bundles extracted from the grass	655:692	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	4	8	theme	tensile	730:736	arg1	modulus					738:744	tensile modulus	730:744	tensile modulus	730:744	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	6	9	theme	noise	977:981	arg1	insulation					983:992	high noise insulation	972:992	high noise insulation	972:992	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	5	10	theme	tensile	806:812	arg1	properties					827:836	Both tensile and flexural properties	801:836	Both tensile and flexural properties of polypropylene composites	801:864	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	3	11	theme	lignocellulosic	457:471	arg1	sources					473:479	other lignocellulosic sources	451:479	other lignocellulosic sources	451:479	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	2	12	theme	sabai	264:268	arg1	grass					270:274	The untreated sabai grass	250:274	The untreated sabai grass	250:274	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	2	12	theme	sabai	264:268	arg1	reinforcement					293:305	reinforcement	293:305	reinforcement	293:305	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	5	13	theme	grass	907:911	arg1	ratio					892:896	ratio	892:896	ratio of sabai grass	892:911	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	3	14	theme	grass	431:435	arg1	composition					406:416	the composition	402:416	the composition of the sabai grass	402:435	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	3	14	theme	grass	431:435	arg1	typical					440:446	typical	440:446	typical	440:446	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	0	15	from	grass	39:43	arg1	biocomposites					14:26	biocomposites	14:26	biocomposites	14:26	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	0	15	from	grass	39:43	arg1	Biofibers					0:8	Biofibers	0:8	Biofibers	0:8	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	2	16	theme	untreated	254:262	arg1	grass					270:274	The untreated sabai grass	250:274	The untreated sabai grass	250:274	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	2	16	theme	untreated	254:262	arg1	reinforcement					293:305	reinforcement	293:305	reinforcement	293:305	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	1	17	theme	Natural	75:81	arg1	fibers					93:98	Natural cellulose fibers	75:98	Natural cellulose fibers	75:98	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	5	18	theme	polypropylene	841:853	arg1	composites					855:864	polypropylene composites	841:864	polypropylene composites	841:864	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	6	19	theme	high	972:975	arg1	insulation					983:992	high noise insulation	972:992	high noise insulation	972:992	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	6	20	theme	Polypropylene	914:926	arg1	composites					928:937	Polypropylene composites	914:937	Polypropylene composites reinforced with sabai grass	914:965	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	0	21	dep	resource	65:72	arg1	biocomposites					14:26	biocomposites	14:26	biocomposites	14:26	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	0	21	dep	resource	65:72	arg1	Biofibers					0:8	Biofibers	0:8	Biofibers	0:8	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	3	22	theme	sabai	425:429	arg1	grass					431:435	the sabai grass	421:435	the sabai grass	421:435	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	4	23	theme	common	768:773	arg1	fibers					793:798	common natural cellulose fibers	768:798	common natural cellulose fibers	768:798	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	3	24	theme	other	451:455	arg1	sources					473:479	other lignocellulosic sources	451:479	other lignocellulosic sources	451:479	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	1	25	theme	cellulose	83:91	arg1	fibers					93:98	Natural cellulose fibers	75:98	Natural cellulose fibers	75:98	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	3	26	theme	flavonoids	509:518	arg1	content					498:504	a high content	491:504	a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed	491:652	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	3	27	theme	phenols	535:541	arg1	content					498:504	a high content	491:504	a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed	491:652	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	1	28	theme	growing	127:133	arg1	binata					163:168	a fast growing perennial grass Eulaliopsis binata	120:168	a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai)	120:194	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	6	29	theme	sabai	955:959	arg1	grass					961:965	sabai grass	955:965	sabai grass	955:965	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	4	30	theme	cellulose	783:791	arg1	fibers					793:798	common natural cellulose fibers	768:798	common natural cellulose fibers	768:798	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	5	31	theme	composites	855:864	arg1	properties					827:836	Both tensile and flexural properties	801:836	Both tensile and flexural properties of polypropylene composites	801:864	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	0	32	theme	sabai	33:37	arg1	grass					39:43	sabai grass	33:43	sabai grass	33:43	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	1	33	dep	known	180:184	arg1	commonly					171:178	commonly	171:178	commonly	171:178	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	5	34	theme	flexural	818:825	arg1	properties					827:836	Both tensile and flexural properties	801:836	Both tensile and flexural properties of polypropylene composites	801:864	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	1	35	dep	binata	163:168	arg1	known					180:184	known	180:184	known as Sabai	180:193	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	6	36	theme	building	1076:1083	arg1	applications					1085:1096	automotive and building applications	1061:1096	automotive and building applications	1061:1096	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	3	37	theme	high	493:496	arg1	content					498:504	a high content	491:504	a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed	491:652	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	5	38	theme	sabai	901:905	arg1	grass					907:911	sabai grass	901:911	sabai grass	901:911	Both tensile and flexural properties of polypropylene composites increased with increasing ratio of sabai grass.
31221327	4	39	theme	natural	775:781	arg1	fibers					793:798	common natural cellulose fibers	768:798	common natural cellulose fibers	768:798	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	3	40	theme	high	569:572	arg1	properties					604:613	high antibacterial, and antifungal properties	569:613	high antibacterial, and antifungal properties	569:613	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	2	41	theme	polypropylene	311:323	arg1	composites					325:334	polypropylene composites	311:334	polypropylene composites	311:334	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	2	42	used	used	285:288	arg2	reinforcement					293:305	reinforcement	293:305	reinforcement	293:305	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	2	42	used	used	285:288	arg2	grass					270:274	The untreated sabai grass	250:274	The untreated sabai grass	250:274	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	4	43	theme	21 GPa	749:754	arg1	strength					706:713	tensile strength	698:713	tensile strength of 493 MPa and tensile modulus of 21 GPa	698:754	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	3	44	theme	antifungal	593:602	arg1	properties					604:613	high antibacterial, and antifungal properties	569:613	high antibacterial, and antifungal properties	569:613	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	1	45	theme	fast	122:125	arg1	binata					163:168	a fast growing perennial grass Eulaliopsis binata	120:168	a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai)	120:194	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	4	46	theme	tensile	698:704	arg1	strength					706:713	tensile strength	698:713	tensile strength of 493 MPa and tensile modulus of 21 GPa	698:754	Fiber bundles extracted from the grass had tensile strength of 493 MPa and tensile modulus of 21 GPa, similar to common natural cellulose fibers.
31221327	0	47	theme	renewable	55:63	arg1	resource					65:72	A unique renewable resource	46:72	A unique renewable resource	46:72	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	3	48	theme	antibacterial	574:586	arg1	properties					604:613	high antibacterial, and antifungal properties	569:613	high antibacterial, and antifungal properties	569:613	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31221327	2	49	theme	composites	358:367	arg1	properties					340:349	properties	340:349	properties of the composites	340:367	The untreated sabai grass has been used as reinforcement for polypropylene composites and properties of the composites have been investigated.
31221327	0	50	theme	unique	48:53	arg1	resource					65:72	A unique renewable resource	46:72	A unique renewable resource	46:72	Biofibers and biocomposites from sabai grass: A unique renewable resource.
31221327	6	51	theme	resistance	1006:1015	arg1	properties					1017:1026	thermal resistance properties	998:1026	thermal resistance properties	998:1026	Polypropylene composites reinforced with sabai grass show high noise insulation and thermal resistance properties suggesting their suitability for automotive and building applications.
31221327	1	52	theme	perennial	135:143	arg1	binata					163:168	a fast growing perennial grass Eulaliopsis binata	120:168	a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai)	120:194	Natural cellulose fibers were extracted from a fast growing perennial grass Eulaliopsis binata (commonly known as Sabai) and characterized for their structure and properties.
31221327	3	53	dep	fibers	622:627	arg1	the					618:620	the	618:620	the	618:620	Although the composition of the sabai grass is typical to other lignocellulosic sources, there is a high content of flavonoids (630 mg/g) and phenols (510 mg/g) which provides high antibacterial, and antifungal properties to the fibers and composites developed.
31252328	6	0	from	resolutions	1018:1028	arg1	parameters					1052:1061	different printing parameters	1033:1061	different printing parameters	1033:1061	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	10	1	theme	3D	1881:1882	arg1	scaffolds					1893:1901	3D hydrogel scaffolds	1881:1901	3D hydrogel scaffolds using bioprinting techniques	1881:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	9	2	theme	3D	1548:1549	arg1	scaffolds					1582:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	10	3	with	bioink	1800:1805	arg1	properties					1834:1843	remarkable mechanical properties	1812:1843	remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1812:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	3	4	theme	3D	597:598	arg1	bioprinting					600:610	extrusion-based 3D bioprinting	581:610	extrusion-based 3D bioprinting	581:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	9	5	theme	composite	1572:1580	arg1	scaffolds					1582:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	6	6	theme	alginate/carrageenan	977:996	arg1	hydrogels					998:1006	alginate and alginate/carrageenan hydrogels	964:1006	alginate and alginate/carrageenan hydrogels	964:1006	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	4	7	theme	shear	707:711	arg1	modulus					713:719	shear modulus	707:719	shear modulus of alginate-based hydrogels	707:747	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	10	8	theme	bioprinting	1909:1919	arg1	techniques					1921:1930	bioprinting techniques	1909:1930	bioprinting techniques	1909:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	6	9	theme	alginate	964:971	arg1	hydrogels					998:1006	alginate and alginate/carrageenan hydrogels	964:1006	alginate and alginate/carrageenan hydrogels	964:1006	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	2	10	theme	uncontrollable	319:332	arg1	properties					345:354	weak and uncontrollable mechanical properties	310:354	weak and uncontrollable mechanical properties of various hydrogels	310:375	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	1	11	theme	tissue	199:204	arg1	engineering					206:216	tissue engineering	199:216	tissue engineering	199:216	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	7	12	theme	printing	1204:1211	arg1	parameters					1213:1222	various printing parameters	1196:1222	various printing parameters	1196:1222	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	2	13	theme	weak	310:313	arg1	properties					345:354	weak and uncontrollable mechanical properties	310:354	weak and uncontrollable mechanical properties of various hydrogels	310:375	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	9	14	theme	printing	1617:1624	arg1	parameters					1626:1635	optimized printing parameters	1607:1635	optimized printing parameters	1607:1635	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	8	15	theme	hydrogels	1353:1361	arg1	deposition					1300:1309	3D deposition	1297:1309	3D deposition of both alginate and alginate/carrageenan hydrogels	1297:1361	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	10	16	theme	carrageenan	1770:1780	arg1	potential					1752:1760	the potential uses	1748:1765	the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1748:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	1	17	theme	medicine	235:242	arg1	field					190:194	the field	186:194	the field of tissue engineering and regenerative medicine	186:242	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	8	18	theme	shape	1434:1438	arg1	fidelity					1440:1447	shape fidelity	1434:1447	shape fidelity	1434:1447	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	6	19	theme	different	1033:1041	arg1	parameters					1052:1061	different printing parameters	1033:1061	different printing parameters	1033:1061	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	2	20	theme	structures	277:286	arg1	printing					262:269	precise printing	254:269	precise printing of 3D structures	254:286	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	7	21	theme	alginate/carrageenan	1144:1163	arg1	scaffolds					1165:1173	alginate and alginate/carrageenan scaffolds	1131:1173	alginate and alginate/carrageenan scaffolds	1131:1173	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	2	22	theme	3D	274:275	arg1	structures					277:286	3D structures	274:286	3D structures	274:286	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	8	23	from	monitoring	1420:1429	arg1	structures					1455:1464	3D structures	1452:1464	3D structures in ten layers and similar printing resolution	1452:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	9	24	theme	live/dead	1658:1666	arg1	staining					1668:1675	live/dead staining	1658:1675	live/dead staining	1658:1675	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	4	25	theme	hydrogels	739:747	arg1	modulus					713:719	shear modulus	707:719	shear modulus of alginate-based hydrogels	707:747	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	1	26	dep	Three-dimensional	103:119	arg1	3D					122:123	3D	122:123	3D	122:123	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	9	27	theme	fluorescence	1690:1701	arg1	imaging					1703:1709	confocal fluorescence imaging	1681:1709	confocal fluorescence imaging	1681:1709	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	8	28	theme	printing	1492:1499	arg1	resolution					1501:1510	similar printing resolution	1484:1510	similar printing resolution	1484:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	5	29	theme	different	820:828	arg1	concentrations					830:843	different concentrations	820:843	different concentrations of carrageenan	820:858	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	10	30	theme	prospective	1788:1798	arg1	bioink					1800:1805	a prospective bioink	1786:1805	a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1786:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	8	31	from	structures	1455:1464	arg1	layers					1473:1478	ten layers	1469:1478	ten layers	1469:1478	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	8	31	from	structures	1455:1464	arg1	resolution					1501:1510	similar printing resolution	1484:1510	similar printing resolution	1484:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	10	32	theme	suitable	1845:1852	arg1	properties					1834:1843	remarkable mechanical properties	1812:1843	remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1812:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	8	33	dep	hydrogels	1353:1361	arg1	both					1314:1317	both	1314:1317	both	1314:1317	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	8	33	dep	hydrogels	1353:1361	arg1	alginate/carrageenan					1332:1351	alginate/carrageenan	1332:1351	alginate/carrageenan	1332:1351	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	10	34	theme	mechanical	1823:1832	arg1	properties					1834:1843	remarkable mechanical properties	1812:1843	remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1812:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	0	35	theme	alginate	34:41	arg1	hydrogels					43:51	alginate hydrogels	34:51	alginate hydrogels	34:51	Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
31252328	5	36	used	used	864:867	arg2	hydrogels					791:799	alginate/carrageenan composite hydrogels	760:799	alginate/carrageenan composite hydrogels	760:799	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	6	37	theme	shear	948:952	arg1	moduli					954:959	shear moduli	948:959	shear moduli	948:959	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	7	38	used	used	1228:1231	arg2	scaffolds					1165:1173	alginate and alginate/carrageenan scaffolds	1131:1173	alginate and alginate/carrageenan scaffolds	1131:1173	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	2	39	theme	hydrogels	367:375	arg1	properties					345:354	weak and uncontrollable mechanical properties	310:354	weak and uncontrollable mechanical properties of various hydrogels	310:375	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	7	40	theme	simulated	1272:1280	arg1	results					1282:1288	the simulated results	1268:1288	the simulated results	1268:1288	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	10	41	from	results	1722:1728	arg1	study					1737:1741	the study	1733:1741	the study	1733:1741	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	8	42	dep	both	1314:1317	arg1	alginate					1319:1326	alginate	1319:1326	alginate	1319:1326	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	10	43	theme	precise	1858:1864	arg1	fabrication					1866:1876	precise fabrication	1858:1876	precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1858:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	4	44	theme	alginate-based	724:737	arg1	hydrogels					739:747	alginate-based hydrogels	724:747	alginate-based hydrogels	724:747	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	10	45	theme	remarkable	1812:1821	arg1	properties					1834:1843	remarkable mechanical properties	1812:1843	remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1812:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	3	46	theme	alginate/carrageenan	534:553	arg1	scaffolds					565:573	alginate/carrageenan composite scaffolds	534:573	alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting	534:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	0	47	theme	rheological	9:19	arg1	behaviors					21:29	rheological behaviors	9:29	rheological behaviors of alginate hydrogels	9:51	Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
31252328	3	48	theme	fabrication	519:529	arg1	feasibility					496:506	the feasibility	492:506	the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting	492:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	6	49	theme	printing	1009:1016	arg1	resolutions					1018:1028	printing resolutions	1009:1028	printing resolutions in different printing parameters	1009:1061	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	3	50	theme	extrusion-based	581:595	arg1	bioprinting					600:610	extrusion-based 3D bioprinting	581:610	extrusion-based 3D bioprinting	581:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	9	51	theme	alginate/carrageenan	1551:1570	arg1	scaffolds					1582:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	the 3D alginate/carrageenan composite scaffolds	1544:1590	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	6	52	theme	hydrogels	998:1006	arg1	viscosities					932:942	the assessed viscosities	919:942	the assessed viscosities	919:942	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	6	52	theme	hydrogels	998:1006	arg1	moduli					954:959	shear moduli	948:959	shear moduli	948:959	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	1	53	used	used	178:181	arg2	bioprinting					126:136	Three-dimensional (3D) bioprinting	103:136	Three-dimensional (3D) bioprinting using biocompatible materials	103:166	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	4	54	theme	proper	627:632	arg1	concentration					634:646	the proper concentration	623:646	the proper concentration of crosslinking agents	623:669	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	3	55	theme	scaffolds	565:573	arg1	fabrication					519:529	precise fabrication	511:529	precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting	511:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	9	56	theme	scaffolds	1582:1590	arg1	viability					1531:1539	the cell viability	1522:1539	the cell viability	1522:1539	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	8	57	theme	3D	1297:1298	arg1	deposition					1300:1309	3D deposition	1297:1309	3D deposition of both alginate and alginate/carrageenan hydrogels	1297:1361	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	10	58	dep	potential	1752:1760	arg1	uses					1762:1765	uses	1762:1765	uses	1762:1765	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	2	59	theme	mechanical	334:343	arg1	properties					345:354	weak and uncontrollable mechanical properties	310:354	weak and uncontrollable mechanical properties of various hydrogels	310:375	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	4	60	theme	modulus	713:719	arg1	assessment					693:702	the assessment	689:702	the assessment of shear modulus of alginate-based hydrogels	689:747	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	7	61	theme	various	1196:1202	arg1	parameters					1213:1222	various printing parameters	1196:1222	various printing parameters	1196:1222	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	1	62	theme	engineering	206:216	arg1	field					190:194	the field	186:194	the field of tissue engineering and regenerative medicine	186:242	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	2	63	theme	various	359:365	arg1	hydrogels					367:375	various hydrogels	359:375	various hydrogels	359:375	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	5	64	theme	composite	781:789	arg1	hydrogels					791:799	alginate/carrageenan composite hydrogels	760:799	alginate/carrageenan composite hydrogels	760:799	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	2	65	theme	precise	254:260	arg1	printing					262:269	precise printing	254:269	precise printing of 3D structures	254:286	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	1	66	theme	regenerative	222:233	arg1	medicine					235:242	regenerative medicine	222:242	regenerative medicine	222:242	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	8	67	theme	continuous	1409:1418	arg1	monitoring					1420:1429	continuous monitoring	1409:1429	continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution	1409:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	7	68	theme	alginate	1131:1138	arg1	scaffolds					1165:1173	alginate and alginate/carrageenan scaffolds	1131:1173	alginate and alginate/carrageenan scaffolds	1131:1173	In addition, alginate and alginate/carrageenan scaffolds were bioprinted with various printing parameters and used to compare their printability with the simulated results.
31252328	6	69	theme	printing	1043:1050	arg1	parameters					1052:1061	different printing parameters	1033:1061	different printing parameters	1033:1061	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	6	70	attach	presented	1082:1090	arg1	maps					1112:1115	the printability maps	1095:1115	the printability maps	1095:1115	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	6	70	attach	presented	1082:1090	arg2	resolutions					1018:1028	printing resolutions	1009:1028	printing resolutions in different printing parameters	1009:1061	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	1	71	theme	biocompatible	144:156	arg1	materials					158:166	biocompatible materials	144:166	biocompatible materials	144:166	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	8	72	theme	fidelity	1440:1447	arg1	monitoring					1420:1429	continuous monitoring	1409:1429	continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution	1409:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	0	73	theme	extrusion-based	74:88	arg1	bioprinting					90:100	extrusion-based bioprinting	74:100	extrusion-based bioprinting	74:100	Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
31252328	9	74	theme	optimized	1607:1615	arg1	parameters					1626:1635	optimized printing parameters	1607:1635	optimized printing parameters	1607:1635	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	8	75	theme	3D	1452:1453	arg1	structures					1455:1464	3D structures	1452:1464	3D structures in ten layers and similar printing resolution	1452:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	5	76	theme	carrageenan	848:858	arg1	concentrations					830:843	different concentrations	820:843	different concentrations of carrageenan	820:858	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	9	77	theme	confocal	1681:1688	arg1	imaging					1703:1709	confocal fluorescence imaging	1681:1709	confocal fluorescence imaging	1681:1709	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	5	78	theme	alginate/carrageenan	760:779	arg1	hydrogels					791:799	alginate/carrageenan composite hydrogels	760:799	alginate/carrageenan composite hydrogels	760:799	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	6	79	theme	printability	1099:1110	arg1	maps					1112:1115	the printability maps	1095:1115	the printability maps	1095:1115	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	8	80	theme	similar	1484:1490	arg1	resolution					1501:1510	similar printing resolution	1484:1510	similar printing resolution	1484:1510	Also, 3D deposition of both alginate and alginate/carrageenan hydrogels were assessed and compared with each other by continuous monitoring of shape fidelity in 3D structures in ten layers and similar printing resolution.
31252328	2	81	theme	preclinical	411:421	arg1	applications					436:447	preclinical and clinical applications	411:447	preclinical and clinical applications	411:447	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	5	82	theme	rheological	886:896	arg1	properties					898:907	their rheological properties	880:907	their rheological properties	880:907	Moreover, alginate/carrageenan composite hydrogels were prepared with different concentrations of carrageenan and used to measure their rheological properties.
31252328	1	83	theme	Three-dimensional	103:119	arg1	bioprinting					126:136	Three-dimensional (3D) bioprinting	103:136	Three-dimensional (3D) bioprinting using biocompatible materials	103:166	Three-dimensional (3D) bioprinting using biocompatible materials is widely used in the field of tissue engineering and regenerative medicine.
31252328	0	84	theme	hydrogels	43:51	arg1	behaviors					21:29	rheological behaviors	9:29	rheological behaviors of alginate hydrogels	9:51	Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
31252328	2	85	theme	clinical	427:434	arg1	applications					436:447	preclinical and clinical applications	411:447	preclinical and clinical applications	411:447	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	2	86	from	potential	398:406	arg1	applications					436:447	preclinical and clinical applications	411:447	preclinical and clinical applications	411:447	However, precise printing of 3D structures is challenging due to weak and uncontrollable mechanical properties of various hydrogels, thus limiting their potential in preclinical and clinical applications.
31252328	3	87	theme	precise	511:517	arg1	fabrication					519:529	precise fabrication	511:529	precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting	511:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	6	88	theme	assessed	923:930	arg1	viscosities					932:942	the assessed viscosities	919:942	the assessed viscosities	919:942	Based on the assessed viscosities and shear moduli of alginate and alginate/carrageenan hydrogels, printing resolutions in different printing parameters were simulated and presented in the printability maps.
31252328	4	89	theme	crosslinking	651:662	arg1	agents					664:669	crosslinking agents	651:669	crosslinking agents	651:669	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	3	90	theme	composite	555:563	arg1	scaffolds					565:573	alginate/carrageenan composite scaffolds	534:573	alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting	534:610	In this study, our goal is to demonstrate the feasibility of precise fabrication of alginate/carrageenan composite scaffolds using extrusion-based 3D bioprinting.
31252328	9	91	theme	cell	1526:1529	arg1	viability					1531:1539	the cell viability	1522:1539	the cell viability	1522:1539	Finally, the cell viability of the 3D alginate/carrageenan composite scaffolds, printed using optimized printing parameters, was evaluated using live/dead staining and confocal fluorescence imaging.
31252328	10	92	theme	hydrogel	1884:1891	arg1	scaffolds					1893:1901	3D hydrogel scaffolds	1881:1901	3D hydrogel scaffolds using bioprinting techniques	1881:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
31252328	4	93	theme	agents	664:669	arg1	concentration					634:646	the proper concentration	623:646	the proper concentration of crosslinking agents	623:669	At first, the proper concentration of crosslinking agents was determined by the assessment of shear modulus of alginate-based hydrogels.
31252328	10	94	theme	scaffolds	1893:1901	arg1	fabrication					1866:1876	precise fabrication	1858:1876	precise fabrication of 3D hydrogel scaffolds using bioprinting techniques	1858:1930	Thus, the results in the study show the potential uses of carrageenan for a prospective bioink with remarkable mechanical properties suitable for precise fabrication of 3D hydrogel scaffolds using bioprinting techniques.
30367558	4	0	theme	surface	867:873	arg1	structures					875:884	the IBS surface structures	859:884	the IBS surface structures	859:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	8	1	dep	content	1366:1372	arg1	found					1378:1382	found	1378:1382	found in some samples	1378:1398	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	11	2	dep	Res	1807:1809	arg1	106A					1819:1822	106A	1819:1822	106A	1819:1822	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.
30367558	11	2	dep	Res	1807:1809	arg1	A					1816:1816	Part A	1811:1816	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.	1792:1840	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.
30367558	1	3	theme	tissue	270:275	arg1	reduction					283:291	a reduction	281:291	a reduction in microbial growth	281:311	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	1	3	theme	tissue	270:275	arg1	regeneration					235:246	the efficient regeneration	221:246	the efficient regeneration of lost or non-viable tissue	221:275	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	2	4	theme	engineered	398:407	arg1	material					420:427	an engineered biomimetic material	395:427	an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses	395:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	2	4	theme	engineered	398:407	arg1	biocompatible					437:449	biocompatible	437:449	biocompatible	437:449	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	8	5	theme	bacteria	1483:1490	arg1	strains					1492:1498	other bacteria strains	1477:1498	other bacteria strains	1477:1498	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	4	6	theme	IBS	863:865	arg1	structures					875:884	the IBS surface structures	859:884	the IBS surface structures	859:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	11	7	theme	Part	1811:1814	arg1	A					1816:1816	Part A	1811:1816	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.	1792:1840	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.
30367558	2	8	theme	non-essential	532:544	arg1	responses					572:580	any non-essential or undesirable biological responses	528:580	any non-essential or undesirable biological responses	528:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	7	9	theme	Gram-positive	1331:1343	arg1	strains					1345:1351	both Gram-negative and Gram-positive strains	1308:1351	both Gram-negative and Gram-positive strains	1308:1351	Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains.
30367558	4	10	theme	chemical	774:781	arg1	cross-linking					783:795	chemical cross-linking	774:795	chemical cross-linking	774:795	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	5	11	theme	bone	1043:1046	arg1	cells					1056:1060	bone forming cells	1043:1060	bone forming cells	1043:1060	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	5	11	theme	bone	1043:1046	arg1	osteoblasts					1027:1037	human osteoblasts	1021:1037	human osteoblasts (or bone forming cells)	1021:1061	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	9	12	theme	innovative	1587:1596	arg1	composites					1573:1582	CG/nHA composites	1566:1582	CG/nHA composites	1566:1582	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	9	12	theme	innovative	1587:1596	arg1	biomaterials					1598:1609	innovative biomaterials	1587:1609	innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs	1587:1758	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	5	13	with	interactions	1003:1014	arg1	cells					1056:1060	bone forming cells	1043:1060	bone forming cells	1043:1060	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	5	13	with	interactions	1003:1014	arg1	osteoblasts					1027:1037	human osteoblasts	1021:1037	human osteoblasts (or bone forming cells)	1021:1061	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	4	14	theme	injectable	733:742	arg1	composites					744:753	injectable composites	733:753	injectable composites	733:753	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	2	15	theme	biological	561:570	arg1	responses					572:580	any non-essential or undesirable biological responses	528:580	any non-essential or undesirable biological responses	528:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	1	16	theme	accelerated	181:191	arg1	periods					202:208	accelerated recovery periods	181:208	accelerated recovery periods	181:208	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	9	17	theme	bacteria	1654:1661	arg1	activity					1663:1670	reduced bacteria activity	1646:1670	reduced bacteria activity	1646:1670	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	0	18	dep	carrageenan	90:100	arg1	hydroxyapatite					111:124	hydroxyapatite	111:124	hydroxyapatite	111:124	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	1	19	theme	recovery	193:200	arg1	periods					202:208	accelerated recovery periods	181:208	accelerated recovery periods	181:208	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	8	20	theme	CG	1363:1364	arg1	content					1366:1372	A higher CG content	1354:1372	A higher CG content	1354:1372	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	9	21	theme	pharmaceutical	1739:1752	arg1	drugs					1754:1758	pharmaceutical drugs	1739:1758	pharmaceutical drugs	1739:1758	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	2	22	theme	long-term	333:341	arg1	success					343:349	the long-term success	329:349	the long-term success of an implant	329:363	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	5	23	theme	forming	1048:1054	arg1	cells					1056:1060	bone forming cells	1043:1060	bone forming cells	1043:1060	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	5	23	theme	forming	1048:1054	arg1	osteoblasts					1027:1037	human osteoblasts	1021:1037	human osteoblasts (or bone forming cells)	1021:1061	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	8	24	theme	growth	1449:1454	arg1	inhibition					1456:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	6	25	from	viability	1125:1133	arg1	IBS					1142:1144	the IBS	1138:1144	the IBS	1138:1144	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	4	26	theme	bacteria	847:854	arg1	behavior					815:822	the in vitro behavior	802:822	the in vitro behavior of mammalian cells and bacteria on the IBS surface structures	802:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	2	27	from	site	489:492	arg1	material					420:427	an engineered biomimetic material	395:427	an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses	395:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	2	27	from	site	489:492	arg1	biocompatible					437:449	biocompatible	437:449	biocompatible	437:449	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	1	28	theme	microbial	296:304	arg1	growth					306:311	microbial growth	296:311	microbial growth	296:311	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	9	29	theme	promoted	1676:1683	arg1	responses					1696:1704	promoted osteoblast responses	1676:1704	promoted osteoblast responses	1676:1704	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	8	30	theme	higher	1356:1361	arg1	content					1366:1372	A higher CG content	1354:1372	A higher CG content	1354:1372	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	6	31	theme	formulation	1187:1197	arg1	types					1199:1203	various formulation types	1179:1203	various formulation types	1179:1203	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	5	32	dep	nHA	959:961	arg1	%					957:957	%	957:957	%	957:957	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	0	33	theme	cytotoxicity	14:25	arg1	activity					45:52	antimicrobial activity	31:52	antimicrobial activity of an injectable bone	31:74	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	0	33	theme	cytotoxicity	14:25	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of cytotoxicity	0:25	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	1	34	from	reduction	283:291	arg1	growth					306:311	microbial growth	296:311	microbial growth	296:311	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	5	35	theme	human	1021:1025	arg1	cells					1056:1060	bone forming cells	1043:1060	bone forming cells	1043:1060	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	5	35	theme	human	1021:1025	arg1	osteoblasts					1027:1037	human osteoblasts	1021:1037	human osteoblasts (or bone forming cells)	1021:1061	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	6	36	from	adhesion	1112:1119	arg1	IBS					1142:1144	the IBS	1138:1144	the IBS	1138:1144	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	6	37	theme	osteoblast	1101:1110	arg1	adhesion					1112:1119	osteoblast adhesion	1101:1119	osteoblast adhesion	1101:1119	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	7	38	theme	antimicrobial	1252:1264	arg1	functions					1266:1274	antimicrobial functions	1252:1274	antimicrobial functions	1252:1274	Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains.
30367558	8	39	dep	Pseudomonas	1426:1436	arg1	aeruginosa					1438:1447	aeruginosa	1438:1447	aeruginosa	1438:1447	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	2	40	theme	undesirable	549:559	arg1	responses					572:580	any non-essential or undesirable biological responses	528:580	any non-essential or undesirable biological responses	528:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	1	41	theme	efficient	225:233	arg1	regeneration					235:246	the efficient regeneration	221:246	the efficient regeneration of lost or non-viable tissue	221:275	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	3	42	theme	injectable	615:624	arg1	bone					626:629	an injectable bone substitute	612:640	an injectable bone substitute (IBS)	612:646	The potential for developing an injectable bone substitute (IBS) was investigated here.
30367558	3	42	theme	injectable	615:624	arg1	IBS					643:645	IBS	643:645	IBS	643:645	The potential for developing an injectable bone substitute (IBS) was investigated here.
30367558	3	43	dep	bone	626:629	arg1	substitute					631:640	substitute	631:640	substitute	631:640	The potential for developing an injectable bone substitute (IBS) was investigated here.
30367558	2	44	theme	material	420:427	arg1	selection					382:390	the selection	378:390	the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses	378:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	0	45	theme	antimicrobial	31:43	arg1	activity					45:52	antimicrobial activity	31:52	antimicrobial activity of an injectable bone	31:74	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	8	46	theme	improved	1417:1424	arg1	inhibition					1456:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	6	47	theme	various	1179:1185	arg1	types					1199:1203	various formulation types	1179:1203	various formulation types	1179:1203	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	2	48	theme	biomimetic	409:418	arg1	material					420:427	an engineered biomimetic material	395:427	an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses	395:580	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	2	48	theme	biomimetic	409:418	arg1	biocompatible					437:449	biocompatible	437:449	biocompatible	437:449	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	9	49	theme	CG/nHA	1566:1571	arg1	composites					1573:1582	CG/nHA composites	1566:1582	CG/nHA composites	1566:1582	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	9	49	theme	CG/nHA	1566:1571	arg1	biomaterials					1598:1609	innovative biomaterials	1587:1609	innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs	1587:1758	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	2	50	theme	implantation	497:508	arg1	implantation					497:508	implantation	497:508	implantation	497:508	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	2	50	theme	implantation	497:508	arg1	site					489:492	the site	485:492	the site of implantation	485:508	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	4	51	theme	cells	837:841	arg1	behavior					815:822	the in vitro behavior	802:822	the in vitro behavior of mammalian cells and bacteria on the IBS surface structures	802:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	1	52	from	regeneration	235:246	arg1	growth					306:311	microbial growth	296:311	microbial growth	296:311	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	2	53	theme	implant	357:363	arg1	success					343:349	the long-term success	329:349	the long-term success of an implant	329:363	Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses.
30367558	8	54	theme	other	1477:1481	arg1	strains					1492:1498	other bacteria strains	1477:1498	other bacteria strains	1477:1498	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	7	55	theme	CG/nHA	1283:1288	arg1	composite					1290:1298	the CG/nHA composite	1279:1298	the CG/nHA composite	1279:1298	Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains.
30367558	11	56	dep	106A	1819:1822	arg1	2984-2993					1825:1833	2984-2993	1825:1833	2984-2993	1825:1833	J Biomed Mater Res Part A: 106A: 2984-2993, 2018.
30367558	4	57	theme	mammalian	827:835	arg1	cells					837:841	mammalian cells	827:841	mammalian cells	827:841	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	6	58	theme	viability	1068:1076	arg1	testing					1078:1084	MTS viability testing	1064:1084	MTS viability testing	1064:1084	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	7	59	theme	Gram-negative	1313:1325	arg1	strains					1345:1351	both Gram-negative and Gram-positive strains	1308:1351	both Gram-negative and Gram-positive strains	1308:1351	Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains.
30367558	8	60	theme	Pseudomonas	1426:1436	arg1	inhibition					1456:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	improved Pseudomonas aeruginosa growth inhibition	1417:1465	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	4	61	dep	carrageenan	686:696	arg1	composites					744:753	injectable composites	733:753	injectable composites	733:753	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	4	62	from	behavior	815:822	arg1	structures					875:884	the IBS surface structures	859:884	the IBS surface structures	859:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	1	63	theme	successful	129:138	arg1	implant					154:160	A successful post-surgical implant	127:160	A successful post-surgical implant	127:160	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	1	64	dep	tissue	270:275	arg1	non-viable					259:268	non-viable	259:268	non-viable	259:268	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	1	64	dep	tissue	270:275	arg1	lost					251:254	lost	251:254	lost	251:254	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	5	65	dep	%	930:930	arg1	nHA					959:961	CG and 60% nHA	948:961	CG and 60% nHA	948:961	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	1	66	theme	post-surgical	140:152	arg1	implant					154:160	A successful post-surgical implant	127:160	A successful post-surgical implant	127:160	A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth.
30367558	6	67	theme	MTS	1064:1066	arg1	testing					1078:1084	MTS viability testing	1064:1084	MTS viability testing	1064:1084	MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types.
30367558	0	68	theme	bone	71:74	arg1	activity					45:52	antimicrobial activity	31:52	antimicrobial activity of an injectable bone	31:74	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	0	68	theme	bone	71:74	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of cytotoxicity	0:25	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	5	69	dep	%	957:957	arg1	60					955:956	60	955:956	60	955:956	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	5	69	dep	%	957:957	arg1	CG					948:949	CG	948:949	CG	948:949	Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells).
30367558	10	70	dep	©	1761:1761	arg1	Inc.					1787:1790	Inc.	1787:1790	Inc.	1787:1790	© 2018 Wiley Periodicals, Inc.
30367558	7	71	theme	Bacteria	1206:1213	arg1	assays					1215:1220	Bacteria assays	1206:1220	Bacteria assays	1206:1220	Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains.
30367558	4	72	dep	in	806:807	arg1	vitro					809:813	vitro	809:813	vitro	809:813	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	8	73	dep	appeared	1500:1507	arg1	unaffected					1509:1518	unaffected	1509:1518	appeared unaffected by the IBS	1500:1529	A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS.
30367558	0	74	theme	injectable	60:69	arg1	bone					71:74	an injectable bone	57:74	an injectable bone	57:74	Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
30367558	9	75	theme	reduced	1646:1652	arg1	activity					1663:1670	reduced bacteria activity	1646:1670	reduced bacteria activity	1646:1670	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
30367558	4	76	theme	in	806:807	arg1	behavior					815:822	the in vitro behavior	802:822	the in vitro behavior of mammalian cells and bacteria on the IBS surface structures	802:884	In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated.
30367558	9	77	theme	osteoblast	1685:1694	arg1	responses					1696:1704	promoted osteoblast responses	1676:1704	promoted osteoblast responses	1676:1704	In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs.
31846657	8	0	theme	burst	1365:1369	arg1	1265 ± 18.9 g					1398:1410	1265 ± 18.9 g	1398:1410	1265 ± 18.9 g	1398:1410	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	0	theme	burst	1365:1369	arg1	strength					1371:1378	burst strength	1365:1378	burst strength (943.56 ± 18.1 to 1265 ± 18.9 g)	1365:1411	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	6	1	theme	casting	955:961	arg1	method					963:968	the solution casting method	942:968	the solution casting method	942:968	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	1	2	theme	material	142:149	arg1	use					116:118	The excessive use	102:118	The excessive use of disposable plastic material in our society	102:164	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	8	3	from	concentration	1263:1275	arg1	incorporation					1231:1243	the incorporation	1227:1243	the incorporation of nano starch at concentration of 0.5 to 10.0%	1227:1291	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	6	4	theme	nano	913:916	arg1	starch					918:923	nano starch	913:923	nano starch	913:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	5	5	theme	mung	735:738	arg1	bean					740:743	mung bean	735:743	mung bean nano starch	735:755	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	5	6	theme	Mung	635:638	arg1	bean					640:643	Mung bean	635:643	Mung bean native starch granules	635:666	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	7	7	theme	native	975:980	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	7	theme	native	975:980	arg1	solubility					1143:1152	water solubility	1137:1152	water solubility (38.49 ± 0.51%)	1137:1168	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	7	theme	native	975:980	arg1	strength					1180:1187	burst strength	1174:1187	burst strength (868.49 ± 26.5 g)	1174:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	7	theme	native	975:980	arg1	properties					994:1003	The native starch film properties	971:1003	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g)	971:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	7	theme	native	975:980	arg1	content					1052:1058	moisture content	1043:1058	moisture content (8.03 ± 0.26%)	1043:1073	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	7	theme	native	975:980	arg1	thickness					1013:1021	thickness	1013:1021	thickness (0.040 ± 0.010 mm)	1013:1040	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	5	8	theme	smooth	696:701	arg1	surface					703:709	a smooth surface	694:709	a smooth surface	694:709	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	5	9	theme	bean	640:643	arg1	granules					659:666	Mung bean native starch granules	635:666	Mung bean native starch granules	635:666	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	6	10	theme	Nano	824:827	arg1	films					852:856	Nano starch-based composite films	824:856	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	824:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	3	11	theme	morphological	508:520	arg1	properties					540:549	it's physicochemical, morphological, and film-forming properties	486:549	it's physicochemical, morphological, and film-forming properties	486:549	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	8	12	dep	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	arg1	to					1513:1514	to	1513:1514	to	1513:1514	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	4	13	theme	average	569:575	arg1	141.772 nm					623:632	141.772 nm	623:632	141.772 nm	623:632	The average particle size distribution of nano starch was 141.772 nm.
31846657	4	13	theme	average	569:575	arg1	distribution					591:602	The average particle size distribution	565:602	The average particle size distribution of nano starch	565:617	The average particle size distribution of nano starch was 141.772 nm.
31846657	0	14	theme	Vigna	86:90	arg1	bean					80:83	mung bean	75:83	mung bean (Vigna radiata)	75:99	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	0	14	theme	Vigna	86:90	arg1	radiata					92:98	Vigna radiata	86:98	Vigna radiata	86:98	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	6	15	theme	varying	863:869	arg1	%					907:907	0.5, 1, 2, 5, and 10%	887:907	0.5, 1, 2, 5, and 10%	887:907	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	6	15	theme	varying	863:869	arg1	concentrations					871:884	varying concentrations	863:884	varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	863:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	5	16	theme	native	645:650	arg1	granules					659:666	Mung bean native starch granules	635:666	Mung bean native starch granules	635:666	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	2	17	theme	biodegradable	348:360	arg1	material					372:379	biodegradable packaging material	348:379	biodegradable packaging material	348:379	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	7	18	theme	water	1076:1080	arg1	5.982 × 10-3 ± 0.30 g-2 s-1					1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	18	theme	water	1076:1080	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	5	19	theme	irregular	795:803	arg1	surface					815:821	irregular and rough surface	795:821	irregular and rough surface	795:821	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	4	20	theme	nano	607:610	arg1	starch					612:617	nano starch	607:617	nano starch	607:617	The average particle size distribution of nano starch was 141.772 nm.
31846657	3	21	theme	physicochemical	491:505	arg1	properties					540:549	it's physicochemical, morphological, and film-forming properties	486:549	it's physicochemical, morphological, and film-forming properties	486:549	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	3	22	theme	mung	429:432	arg1	bean					434:437	mung bean	429:437	mung bean	429:437	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	7	23	theme	moisture	1043:1050	arg1	content					1052:1058	moisture content	1043:1058	moisture content (8.03 ± 0.26%)	1043:1073	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	23	theme	moisture	1043:1050	arg1	%					1072:1072	8.03 ± 0.26%	1061:1072	8.03 ± 0.26%	1061:1072	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	1	24	from	use	116:118	arg1	society					158:164	society	158:164	society	158:164	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	6	25	theme	composite	842:850	arg1	films					852:856	Nano starch-based composite films	824:856	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	824:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	7	26	theme	film	989:992	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	26	theme	film	989:992	arg1	solubility					1143:1152	water solubility	1137:1152	water solubility (38.49 ± 0.51%)	1137:1168	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	26	theme	film	989:992	arg1	strength					1180:1187	burst strength	1174:1187	burst strength (868.49 ± 26.5 g)	1174:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	26	theme	film	989:992	arg1	properties					994:1003	The native starch film properties	971:1003	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g)	971:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	26	theme	film	989:992	arg1	content					1052:1058	moisture content	1043:1058	moisture content (8.03 ± 0.26%)	1043:1073	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	26	theme	film	989:992	arg1	thickness					1013:1021	thickness	1013:1021	thickness (0.040 ± 0.010 mm)	1013:1040	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	8	27	dep	10.0	1287:1290	arg1	to					1284:1285	to	1284:1285	to	1284:1285	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	2	28	theme	essential	308:316	arg1	sources					318:324	the essential sources	304:324	the essential sources for the production of biodegradable packaging material	304:379	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	8	29	theme	vapor	1468:1472	arg1	3.364 × 10-3 ± 0.35 g-2 s-1					1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	29	theme	vapor	1468:1472	arg1	rate					1487:1490	water vapor transmission rate	1462:1490	water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1)	1462:1543	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	3	30	theme	present	389:395	arg1	study					397:401	the present study	385:401	the present study	385:401	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	5	31	with	form	785:788	arg1	surface					815:821	irregular and rough surface	795:821	irregular and rough surface	795:821	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	8	32	theme	nano	1248:1251	arg1	starch					1253:1258	nano starch	1248:1258	nano starch	1248:1258	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	1	33	theme	packaging	174:182	arg1	material					184:191	packaging material	174:191	packaging material which can undergo quick degradation without harming the environment	174:259	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	5	34	theme	agglomerated	772:783	arg1	form					785:788	an agglomerated form	769:788	an agglomerated form with irregular and rough surface	769:821	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	6	35	with	films	852:856	arg1	%					907:907	0.5, 1, 2, 5, and 10%	887:907	0.5, 1, 2, 5, and 10%	887:907	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	6	35	with	films	852:856	arg1	concentrations					871:884	varying concentrations	863:884	varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	863:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	5	36	theme	oval	676:679	arg1	shape					681:685	oval shape	676:685	oval shape	676:685	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	3	37	attach	isolated	415:422	arg1	bean					434:437	mung bean	429:437	mung bean	429:437	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	3	37	attach	isolated	415:422	arg2	starch					404:409	starch	404:409	starch	404:409	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	4	38	theme	starch	612:617	arg1	141.772 nm					623:632	141.772 nm	623:632	141.772 nm	623:632	The average particle size distribution of nano starch was 141.772 nm.
31846657	4	38	theme	starch	612:617	arg1	distribution					591:602	The average particle size distribution	565:602	The average particle size distribution of nano starch	565:617	The average particle size distribution of nano starch was 141.772 nm.
31846657	8	39	theme	starch	1253:1258	arg1	incorporation					1231:1243	the incorporation	1227:1243	the incorporation of nano starch at concentration of 0.5 to 10.0%	1227:1291	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	40	dep	0.063 ± 0.006 mm	1346:1361	arg1	to					1343:1344	to	1343:1344	to	1343:1344	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	4	41	theme	size	586:589	arg1	141.772 nm					623:632	141.772 nm	623:632	141.772 nm	623:632	The average particle size distribution of nano starch was 141.772 nm.
31846657	4	41	theme	size	586:589	arg1	distribution					591:602	The average particle size distribution	565:602	The average particle size distribution of nano starch	565:617	The average particle size distribution of nano starch was 141.772 nm.
31846657	6	42	theme	starch-based	829:840	arg1	films					852:856	Nano starch-based composite films	824:856	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	824:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	3	43	theme	film-forming	527:538	arg1	properties					540:549	it's physicochemical, morphological, and film-forming properties	486:549	it's physicochemical, morphological, and film-forming properties	486:549	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	1	44	theme	disposable	123:132	arg1	material					142:149	disposable plastic material	123:149	disposable plastic material	123:149	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	5	45	theme	bean	740:743	arg1	starch					750:755	mung bean nano starch	735:755	mung bean nano starch	735:755	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	3	46	theme	starch	474:479	arg1	synthesis					456:464	the synthesis	452:464	the synthesis of nano starch	452:479	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	0	47	from	bean	80:83	arg1	films					64:68	composite films	54:68	composite films from mung bean (Vigna radiata)	54:99	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	6	48	theme	solution	946:953	arg1	method					963:968	the solution casting method	942:968	the solution casting method	942:968	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	0	49	theme	nano	36:39	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	0	49	theme	nano	36:39	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	5	50	theme	nano	745:748	arg1	starch					750:755	mung bean nano starch	735:755	mung bean nano starch	735:755	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	8	51	dep	4.80 ± 0.48	1447:1457	arg1	to					1444:1445	to	1444:1445	to	1444:1445	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	52	dep	1265 ± 18.9 g	1398:1410	arg1	to					1395:1396	to	1395:1396	to	1395:1396	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	53	dep	34.11 ± 0.40	1577:1588	arg1	to					1574:1575	to	1574:1575	to	1574:1575	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	5	54	theme	rough	809:813	arg1	surface					815:821	irregular and rough surface	795:821	irregular and rough surface	795:821	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	7	55	theme	transmission	1088:1099	arg1	5.982 × 10-3 ± 0.30 g-2 s-1					1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	55	theme	transmission	1088:1099	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	5	56	theme	starch	652:657	arg1	granules					659:666	Mung bean native starch granules	635:666	Mung bean native starch granules	635:666	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	8	57	theme	moisture	1414:1421	arg1	%					1458:1458	6.09 ± 0.28 to 4.80 ± 0.48%	1432:1458	6.09 ± 0.28 to 4.80 ± 0.48%	1432:1458	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	57	theme	moisture	1414:1421	arg1	content					1423:1429	moisture content	1414:1429	moisture content (6.09 ± 0.28 to 4.80 ± 0.48%)	1414:1459	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	58	theme	%	1291:1291	arg1	concentration					1263:1275	concentration	1263:1275	concentration of 0.5 to 10.0%	1263:1291	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	3	59	theme	nano	469:472	arg1	starch					474:479	nano starch	469:479	nano starch	469:479	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31846657	0	60	theme	composite	54:62	arg1	films					64:68	composite films	54:68	composite films from mung bean (Vigna radiata)	54:99	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	7	61	theme	vapor	1082:1086	arg1	5.982 × 10-3 ± 0.30 g-2 s-1					1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	5.982 × 10-3 ± 0.30 g-2 s-1	1107:1133	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	61	theme	vapor	1082:1086	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	1	62	theme	plastic	134:140	arg1	material					142:149	disposable plastic material	123:149	disposable plastic material	123:149	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	1	63	theme	excessive	106:114	arg1	use					116:118	The excessive use	102:118	The excessive use of disposable plastic material in our society	102:164	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	8	64	theme	film	1294:1297	arg1	thickness					1318:1326	thickness	1318:1326	thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm)	1318:1362	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	64	theme	film	1294:1297	arg1	solubility					1549:1558	solubility	1549:1558	solubility (37.99 ± 0.47 to 34.11 ± 0.40%)	1549:1590	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	64	theme	film	1294:1297	arg1	strength					1371:1378	burst strength	1365:1378	burst strength (943.56 ± 18.1 to 1265 ± 18.9 g)	1365:1411	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	64	theme	film	1294:1297	arg1	rate					1487:1490	water vapor transmission rate	1462:1490	water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1)	1462:1543	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	64	theme	film	1294:1297	arg1	properties					1299:1308	film properties	1294:1308	film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%)	1294:1590	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	64	theme	film	1294:1297	arg1	content					1423:1429	moisture content	1414:1429	moisture content (6.09 ± 0.28 to 4.80 ± 0.48%)	1414:1459	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	2	65	theme	Agricultural	262:273	arg1	products					275:282	Agricultural products	262:282	Agricultural products	262:282	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	1	66	theme	quick	211:215	arg1	degradation					217:227	quick degradation	211:227	quick degradation	211:227	The excessive use of disposable plastic material in our society demands packaging material which can undergo quick degradation without harming the environment.
31846657	7	67	theme	starch	982:987	arg1	rate					1101:1104	water vapor transmission rate	1076:1104	water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1)	1076:1134	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	67	theme	starch	982:987	arg1	solubility					1143:1152	water solubility	1137:1152	water solubility (38.49 ± 0.51%)	1137:1168	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	67	theme	starch	982:987	arg1	strength					1180:1187	burst strength	1174:1187	burst strength (868.49 ± 26.5 g)	1174:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	67	theme	starch	982:987	arg1	properties					994:1003	The native starch film properties	971:1003	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g)	971:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	67	theme	starch	982:987	arg1	content					1052:1058	moisture content	1043:1058	moisture content (8.03 ± 0.26%)	1043:1073	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	67	theme	starch	982:987	arg1	thickness					1013:1021	thickness	1013:1021	thickness (0.040 ± 0.010 mm)	1013:1040	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	68	theme	burst	1174:1178	arg1	868.49 ± 26.5 g					1190:1204	868.49 ± 26.5 g	1190:1204	868.49 ± 26.5 g	1190:1204	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	68	theme	burst	1174:1178	arg1	strength					1180:1187	burst strength	1174:1187	burst strength (868.49 ± 26.5 g)	1174:1205	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	2	69	theme	sources	318:324	arg1	sources					318:324	the essential sources	304:324	the essential sources for the production of biodegradable packaging material	304:379	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	2	69	theme	sources	318:324	arg1	one					297:299	one	297:299	one	297:299	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	4	70	theme	particle	577:584	arg1	141.772 nm					623:632	141.772 nm	623:632	141.772 nm	623:632	The average particle size distribution of nano starch was 141.772 nm.
31846657	4	70	theme	particle	577:584	arg1	distribution					591:602	The average particle size distribution	565:602	The average particle size distribution of nano starch	565:617	The average particle size distribution of nano starch was 141.772 nm.
31846657	0	71	theme	mung	75:78	arg1	bean					80:83	mung bean	75:83	mung bean (Vigna radiata)	75:99	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	0	71	theme	mung	75:78	arg1	radiata					92:98	Vigna radiata	86:98	Vigna radiata	86:98	Development and characterization of nano starch-based composite films from mung bean (Vigna radiata).
31846657	5	72	from	cracks	722:727	arg1	free					712:715	free	712:715	free	712:715	Mung bean native starch granules were of oval shape having a smooth surface, free from cracks while mung bean nano starch appeared in an agglomerated form with irregular and rough surface.
31846657	2	73	theme	material	372:379	arg1	production					334:343	the production	330:343	the production of biodegradable packaging material	330:379	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	8	74	theme	transmission	1474:1485	arg1	3.364 × 10-3 ± 0.35 g-2 s-1					1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	74	theme	transmission	1474:1485	arg1	rate					1487:1490	water vapor transmission rate	1462:1490	water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1)	1462:1543	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	75	theme	water	1462:1466	arg1	3.364 × 10-3 ± 0.35 g-2 s-1					1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	3.364 × 10-3 ± 0.35 g-2 s-1	1516:1542	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	8	75	theme	water	1462:1466	arg1	rate					1487:1490	water vapor transmission rate	1462:1490	water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1)	1462:1543	With the incorporation of nano starch at concentration of 0.5 to 10.0%, film properties such as thickness (0.043 ± 0.006 to 0.063 ± 0.006 mm), burst strength (943.56 ± 18.1 to 1265 ± 18.9 g), moisture content (6.09 ± 0.28 to 4.80 ± 0.48%), water vapor transmission rate (5.558 × 10-3 ± 0.25 to 3.364 × 10-3 ± 0.35 g-2 s-1) and solubility (37.99 ± 0.47 to 34.11 ± 0.40%) were improved.
31846657	6	76	theme	starch	918:923	arg1	%					907:907	0.5, 1, 2, 5, and 10%	887:907	0.5, 1, 2, 5, and 10%	887:907	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	6	76	theme	starch	918:923	arg1	concentrations					871:884	varying concentrations	863:884	varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch	863:923	Nano starch-based composite films with varying concentrations (0.5, 1, 2, 5, and 10%) of nano starch were prepared by the solution casting method.
31846657	2	77	theme	packaging	362:370	arg1	material					372:379	biodegradable packaging material	348:379	biodegradable packaging material	348:379	Agricultural products can serve as one of the essential sources for the production of biodegradable packaging material.
31846657	7	78	theme	water	1137:1141	arg1	solubility					1143:1152	water solubility	1137:1152	water solubility (38.49 ± 0.51%)	1137:1168	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	7	78	theme	water	1137:1141	arg1	%					1167:1167	38.49 ± 0.51%	1155:1167	38.49 ± 0.51%	1155:1167	The native starch film properties such as thickness (0.040 ± 0.010 mm), moisture content (8.03 ± 0.26%), water vapor transmission rate (5.982 × 10-3 ± 0.30 g-2 s-1), water solubility (38.49 ± 0.51%) and burst strength (868.49 ± 26.5 g) were observed.
31846657	3	79	used	used	443:446	arg2	starch					404:409	starch	404:409	starch	404:409	In the present study, starch was isolated from mung bean and used for the synthesis of nano starch, and it's physicochemical, morphological, and film-forming properties were studied.
31604180	12	0	theme	size-dependent	1853:1866	arg1	changes					1879:1885	the size-dependent structural changes	1849:1885	the size-dependent structural changes	1849:1885	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	14	1	theme	fibrin	2335:2340	arg1	structure					2342:2350	fibrin structure	2335:2350	fibrin structure	2335:2350	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	1	2	from	mechanism	232:240	arg1	immunity					276:283	the neutrophil-mediated innate immunity	245:283	the neutrophil-mediated innate immunity	245:283	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	4	3	from	range	666:670	arg1	polyphosphates					626:639	polyphosphates	626:639	polyphosphates	626:639	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	4	3	from	range	666:670	arg1	polyanions					614:623	polyanions	614:623	polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers)	614:698	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	1	4	theme	neutrophil-mediated	249:267	arg1	immunity					276:283	the neutrophil-mediated innate immunity	245:283	the neutrophil-mediated innate immunity	245:283	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	6	5	theme	Fibrin	860:865	arg1	structure					867:875	Fibrin structure	860:875	Fibrin structure	860:875	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	10	6	theme	larger	1607:1612	arg1	pores					1626:1630	smaller (LMWH, S5, P700) or larger (UFH, P100) pores	1579:1630	smaller (LMWH, S5, P700) or larger (UFH, P100) pores	1579:1630	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	4	7	theme	size	653:656	arg1	polyphosphates					626:639	polyphosphates	626:639	polyphosphates	626:639	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	4	7	theme	size	653:656	arg1	polyanions					614:623	polyanions	614:623	polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers)	614:698	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	4	8	from	size	653:656	arg1	range					666:670	the range 60-1000 phosphate monomers	662:697	the range 60-1000 phosphate monomers	662:697	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	5	9	theme	molecular	816:824	arg1	size					826:829	its molecular size	812:829	its molecular size	812:829	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	14	10	theme	fibrin	2289:2294	arg1	clots					2296:2300	fibrin clots	2289:2300	fibrin clots	2289:2300	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	8	11	theme	low	1231:1233	arg1	heparin					1252:1258	low molecular-weight heparin	1231:1258	low molecular-weight heparin (LMWH)	1231:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	11	theme	low	1231:1233	arg1	LMWH					1261:1264	LMWH	1261:1264	LMWH	1261:1264	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	13	12	theme	lysine-binding	2177:2190	arg1	plasmin					2201:2207	plasmin	2201:2207	plasmin	2201:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	12	theme	lysine-binding	2177:2190	arg1	sites					2192:2196	the lysine-binding sites	2173:2196	the lysine-binding sites of plasmin	2173:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	0	13	theme	charge	127:132	arg1	Effects					90:96	Effects	90:96	mechanical and proteolytic decomposition: Effects of polymer size and electric charge	48:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	2	14	theme	polyanionic	301:311	arg1	DNA					313:315	the polyanionic DNA	297:315	the polyanionic DNA	297:315	In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components.
31604180	8	15	theme	desulfated	1196:1205	arg1	derivatives					1207:1217	its partially or fully desulfated derivatives	1173:1217	its partially or fully desulfated derivatives	1173:1217	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	12	16	theme	lysis-times	1933:1943	arg1	prolongation					1917:1928	a 10-15% prolongation	1908:1928	a 10-15% prolongation of lysis-times with plasmin	1908:1956	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	10	17	theme	fibrin	1510:1515	arg1	structure					1517:1525	the fibrin structure	1506:1525	the fibrin structure	1506:1525	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	6	18	theme	electron	906:913	arg1	SEM					927:929	SEM	927:929	SEM	927:929	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	6	18	theme	electron	906:913	arg1	microscopy					915:924	scanning electron microscopy	897:924	scanning electron microscopy (SEM)	897:930	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	11	19	theme	large	1769:1773	arg1	UFH					1775:1777	the large UFH	1765:1777	the large UFH	1765:1777	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	7	20	theme	oscillation	967:977	arg1	rheometer					979:987	An oscillation rheometer	964:987	An oscillation rheometer	964:987	An oscillation rheometer was used to investigate viscoelastic properties.
31604180	12	21	theme	10-15	1910:1914	arg1	%					1915:1915	%	1915:1915	%	1915:1915	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	11	22	theme	maximal	1802:1808	arg1	deformation					1819:1829	the maximal bearable deformation	1798:1829	the maximal bearable deformation of clots	1798:1838	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	7	23	used	used	993:996	arg2	rheometer					979:987	An oscillation rheometer	964:987	An oscillation rheometer	964:987	An oscillation rheometer was used to investigate viscoelastic properties.
31604180	5	24	theme	electric	843:850	arg1	charge					852:857	relative electric charge	834:857	relative electric charge	834:857	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	4	25	theme	phosphate	680:688	arg1	monomers					690:697	phosphate monomers	680:697	the range 60-1000 phosphate monomers	662:697	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	0	26	dep	decomposition	75:87	arg1	Effects					90:96	Effects	90:96	mechanical and proteolytic decomposition: Effects of polymer size and electric charge	48:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	0	27	theme	polymer	101:107	arg1	size					109:112	polymer size	101:112	polymer size	101:112	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	11	28	theme	clots	1834:1838	arg1	deformation					1819:1829	the maximal bearable deformation	1798:1829	the maximal bearable deformation of clots	1798:1838	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	6	29	theme	pressure-driven	936:950	arg1	permeation					952:961	pressure-driven permeation	936:961	pressure-driven permeation	936:961	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	8	30	theme	45	1321:1322	arg1	monomers					1356:1363	45 (P45), 100 (P100) or 700 (P700) monomers	1321:1363	45 (P45), 100 (P100) or 700 (P700) monomers at average	1321:1374	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	10	31	theme	clot	1536:1539	arg1	permeation					1541:1550	clot permeation	1536:1550	clot permeation	1536:1550	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	11	32	dep	smaller	1691:1697	arg1	LMWH					1713:1716	LMWH	1713:1716	LMWH	1713:1716	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	11	32	dep	smaller	1691:1697	arg1	P100					1704:1707	P100	1704:1707	P100	1704:1707	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	11	32	dep	smaller	1691:1697	arg1	P45					1699:1701	P45	1699:1701	P45	1699:1701	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	4	33	from	polyanions	614:623	arg1	range					666:670	the range 60-1000 phosphate monomers	662:697	the range 60-1000 phosphate monomers	662:697	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	10	34	dep	smaller	1579:1585	arg1	S5					1594:1595	S5	1594:1595	S5	1594:1595	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	10	34	dep	smaller	1579:1585	arg1	LMWH					1588:1591	LMWH	1588:1591	LMWH	1588:1591	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	10	34	dep	smaller	1579:1585	arg1	P700					1598:1601	P700	1598:1601	P700	1598:1601	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	0	35	theme	Biorelevant	0:10	arg1	polyanions					12:21	Biorelevant polyanions	0:21	Biorelevant polyanions	0:21	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	8	36	theme	turbidimetric	1046:1058	arg1	assays					1060:1065	Kinetic turbidimetric assays	1038:1065	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH)	1038:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	3	37	theme	therapeutic	467:477	arg1	agents					479:484	therapeutic agents	467:484	therapeutic agents	467:484	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	9	38	theme	clot	1467:1470	arg1	formation					1472:1480	clot formation	1467:1480	clot formation	1467:1480	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	2	39	theme	components	414:423	arg1	effects					399:405	the effects	395:405	the effects of NET components	395:423	In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components.
31604180	13	40	theme	%	2016:2016	arg1	phenomenon					2099:2108	a kringle-dependent phenomenon	2079:2108	a kringle-dependent phenomenon	2079:2108	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	40	theme	%	2016:2016	arg1	prolongation					2018:2029	The 20-35% prolongation	2007:2029	The 20-35% prolongation of lysis-times caused by all polyphosphates	2007:2073	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	40	theme	%	2016:2016	arg1	dampened					2119:2126	dampened	2119:2126	dampened	2119:2126	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	14	41	theme	fibrinolysis	2405:2416	arg1	inhibition					2374:2383	kringle-dependent inhibition	2356:2383	kringle-dependent inhibition of plasmin-mediated fibrinolysis	2356:2416	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	14	41	theme	fibrinolysis	2405:2416	arg1	modulation					2321:2330	size-dependent modulation	2306:2330	size-dependent modulation of fibrin structure	2306:2350	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	14	42	theme	structure	2269:2277	arg1	polyanions					2236:2245	polyanions	2236:2245	polyanions of different chemical structure	2236:2277	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	5	43	theme	fibrin	765:770	arg1	stability					752:760	the stability	748:760	the stability of fibrin	748:770	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	8	44	theme	700	1345:1347	arg1	monomers					1356:1363	45 (P45), 100 (P100) or 700 (P700) monomers	1321:1363	45 (P45), 100 (P100) or 700 (P700) monomers at average	1321:1374	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	14	45	theme	different	2250:2258	arg1	structure					2269:2277	different chemical structure	2250:2277	different chemical structure	2250:2277	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	10	46	theme	smaller	1579:1585	arg1	pores					1626:1630	smaller (LMWH, S5, P700) or larger (UFH, P100) pores	1579:1630	smaller (LMWH, S5, P700) or larger (UFH, P100) pores	1579:1630	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	8	47	theme	composite	1105:1113	arg1	clots					1122:1126	composite fibrin clots	1105:1126	composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH)	1105:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	14	48	theme	kringle-dependent	2356:2372	arg1	inhibition					2374:2383	kringle-dependent inhibition	2356:2383	kringle-dependent inhibition of plasmin-mediated fibrinolysis	2356:2416	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	1	49	theme	neutrophil	150:159	arg1	mechanism					232:240	an essential mechanism	219:240	an essential mechanism in the neutrophil-mediated innate immunity	219:283	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	49	theme	neutrophil	150:159	arg1	release					139:145	The release	135:145	The release of neutrophil extracellular traps (NETs) containing DNA and histones	135:214	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	4	50	theme	different	596:604	arg1	type					606:609	a different type	594:609	a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers)	594:698	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	11	51	theme	fibrin	1749:1754	arg1	deformability					1732:1744	the deformability	1728:1744	the deformability of fibrin	1728:1754	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	12	52	theme	sulfation	1987:1995	arg1	patterns					1997:2004	sulfation patterns	1987:2004	sulfation patterns	1987:2004	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	8	53	theme	clots	1122:1126	arg1	generation					1075:1084	the generation	1071:1084	the generation	1071:1084	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	53	theme	clots	1122:1126	arg1	dissolution					1090:1100	dissolution	1090:1100	dissolution	1090:1100	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	14	54	theme	structure	2342:2350	arg1	inhibition					2374:2383	kringle-dependent inhibition	2356:2383	kringle-dependent inhibition of plasmin-mediated fibrinolysis	2356:2416	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	14	54	theme	structure	2342:2350	arg1	modulation					2321:2330	size-dependent modulation	2306:2330	size-dependent modulation of fibrin structure	2306:2350	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	3	55	dep	agents	479:484	arg1	only					459:462	only	459:462	only	459:462	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	8	56	theme	unfractionated	1139:1152	arg1	UFH					1163:1165	UFH	1163:1165	UFH	1163:1165	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	56	theme	unfractionated	1139:1152	arg1	heparin					1154:1160	unfractionated heparin	1139:1160	unfractionated heparin (UFH)	1139:1166	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	3	57	theme	entry	531:535	arg1	pathogens					546:554	pathogens	546:554	pathogens	546:554	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	3	57	theme	entry	531:535	arg1	sites					537:541	entry sites	531:541	entry sites of pathogens	531:554	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	1	58	theme	innate	269:274	arg1	immunity					276:283	the neutrophil-mediated innate immunity	245:283	the neutrophil-mediated innate immunity	245:283	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	14	59	theme	size-dependent	2306:2319	arg1	modulation					2321:2330	size-dependent modulation	2306:2330	size-dependent modulation of fibrin structure	2306:2350	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	5	60	theme	charge	852:857	arg1	type					793:796	the type	789:796	the type of polyanion, its molecular size or relative electric charge	789:857	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	11	61	theme	bearable	1810:1817	arg1	deformation					1819:1829	the maximal bearable deformation	1798:1829	the maximal bearable deformation of clots	1798:1838	All polyanions stabilized the clots mechanically, but the smaller P45, P100 and LMWH decreased the deformability of fibrin, whereas the large UFH and P700 increased the maximal bearable deformation of clots.
31604180	13	62	theme	6-aminohexanoate	2147:2162	arg1	presence					2135:2142	the presence	2131:2142	the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin	2131:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	4	63	theme	various	645:651	arg1	size					653:656	various size	645:656	various size (in the range 60-1000 phosphate monomers)	645:698	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	13	64	from	dampened	2119:2126	arg1	presence					2135:2142	the presence	2131:2142	the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin	2131:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	2	65	theme	mechanical	325:334	arg1	resistance					346:355	mechanical and lytic resistance	325:355	mechanical and lytic resistance to fibrin	325:365	In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components.
31604180	5	66	theme	size	826:829	arg1	type					793:796	the type	789:796	the type of polyanion, its molecular size or relative electric charge	789:857	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	12	67	theme	%	1915:1915	arg1	prolongation					1917:1928	a 10-15% prolongation	1908:1928	a 10-15% prolongation of lysis-times with plasmin	1908:1956	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	5	68	theme	polyanion	801:809	arg1	type					793:796	the type	789:796	the type of polyanion, its molecular size or relative electric charge	789:857	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	7	69	theme	viscoelastic	1013:1024	arg1	properties					1026:1035	viscoelastic properties	1013:1035	viscoelastic properties	1013:1035	An oscillation rheometer was used to investigate viscoelastic properties.
31604180	6	70	theme	scanning	897:904	arg1	SEM					927:929	SEM	927:929	SEM	927:929	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	6	70	theme	scanning	897:904	arg1	microscopy					915:924	scanning electron microscopy	897:924	scanning electron microscopy (SEM)	897:930	Fibrin structure was approached with scanning electron microscopy (SEM) and pressure-driven permeation.
31604180	2	71	theme	lytic	340:344	arg1	resistance					346:355	mechanical and lytic resistance	325:355	mechanical and lytic resistance to fibrin	325:365	In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components.
31604180	8	72	from	average	1368:1374	arg1	monomers					1356:1363	45 (P45), 100 (P100) or 700 (P700) monomers	1321:1363	45 (P45), 100 (P100) or 700 (P700) monomers at average	1321:1374	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	5	73	theme	relative	834:841	arg1	charge					852:857	relative electric charge	834:857	relative electric charge	834:857	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	12	74	theme	structural	1868:1877	arg1	changes					1879:1885	the size-dependent structural changes	1849:1885	the size-dependent structural changes	1849:1885	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	5	75	theme	current	710:716	arg1	study					718:722	the current study	706:722	the current study	706:722	With the current study we aimed to evaluate if the stability of fibrin is influenced by the type of polyanion, its molecular size or relative electric charge.
31604180	1	76	theme	extracellular	161:173	arg1	neutrophil					150:159	neutrophil extracellular traps	150:179	neutrophil extracellular traps (NETs) containing DNA and histones	150:214	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	76	theme	extracellular	161:173	arg1	NETs					182:185	NETs	182:185	NETs	182:185	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	10	77	dep	larger	1607:1612	arg1	P100					1620:1623	P100	1620:1623	P100	1620:1623	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	10	77	dep	larger	1607:1612	arg1	UFH					1615:1617	UFH	1615:1617	UFH	1615:1617	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	0	78	theme	size	109:112	arg1	Effects					90:96	Effects	90:96	mechanical and proteolytic decomposition: Effects of polymer size and electric charge	48:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	3	79	theme	pathogens	546:554	arg1	pathogens					546:554	pathogens	546:554	pathogens	546:554	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	3	79	theme	pathogens	546:554	arg1	sites					537:541	entry sites	531:541	entry sites of pathogens	531:554	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	13	80	theme	kringle-dependent	2081:2097	arg1	phenomenon					2099:2108	a kringle-dependent phenomenon	2079:2108	a kringle-dependent phenomenon	2079:2108	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	80	theme	kringle-dependent	2081:2097	arg1	prolongation					2018:2029	The 20-35% prolongation	2007:2029	The 20-35% prolongation of lysis-times caused by all polyphosphates	2007:2073	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	80	theme	kringle-dependent	2081:2097	arg1	dampened					2119:2126	dampened	2119:2126	dampened	2119:2126	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	0	81	theme	electric	118:125	arg1	charge					127:132	electric charge	118:132	electric charge	118:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	8	82	theme	Kinetic	1038:1044	arg1	assays					1060:1065	Kinetic turbidimetric assays	1038:1065	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH)	1038:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	83	theme	molecular-weight	1235:1250	arg1	heparin					1252:1258	low molecular-weight heparin	1231:1258	low molecular-weight heparin (LMWH)	1231:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	83	theme	molecular-weight	1235:1250	arg1	LMWH					1261:1264	LMWH	1261:1264	LMWH	1261:1264	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	4	84	dep	range	666:670	arg1	monomers					690:697	phosphate monomers	680:697	the range 60-1000 phosphate monomers	662:697	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	8	85	contain	containing	1128:1137	arg2	heparin					1252:1258	low molecular-weight heparin	1231:1258	low molecular-weight heparin (LMWH)	1231:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	85	contain	containing	1128:1137	arg2	derivatives					1207:1217	its partially or fully desulfated derivatives	1173:1217	its partially or fully desulfated derivatives	1173:1217	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	85	contain	containing	1128:1137	arg2	LMWH					1261:1264	LMWH	1261:1264	LMWH	1261:1264	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	85	contain	containing	1128:1137	arg2	heparin					1154:1160	unfractionated heparin	1139:1160	unfractionated heparin (UFH)	1139:1166	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	85	contain	containing	1128:1137	arg2	UFH					1163:1165	UFH	1163:1165	UFH	1163:1165	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	8	85	contain	containing	1128:1137	arg1	clots					1122:1126	composite fibrin clots	1105:1126	composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH)	1105:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	1	86	contain	containing	188:197	arg1	neutrophil					150:159	neutrophil extracellular traps	150:179	neutrophil extracellular traps (NETs) containing DNA and histones	150:214	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	86	contain	containing	188:197	arg2	histones					207:214	histones	207:214	histones	207:214	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	86	contain	containing	188:197	arg2	DNA					199:201	DNA	199:201	DNA	199:201	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	86	contain	containing	188:197	arg1	NETs					182:185	NETs	182:185	NETs	182:185	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	87	dep	neutrophil	150:159	arg1	traps					175:179	traps	175:179	traps	175:179	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	10	88	theme	thicker	1559:1565	arg1	fibers					1567:1572	thicker fibers	1559:1572	thicker fibers	1559:1572	All polyanions altered the fibrin structure: SEM and clot permeation showed thicker fibers with smaller (LMWH, S5, P700) or larger (UFH, P100) pores.
31604180	0	89	theme	mechanical	48:57	arg1	decomposition					75:87	mechanical and proteolytic decomposition	48:87	mechanical and proteolytic decomposition: Effects of polymer size and electric charge	48:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	9	90	theme	smaller	1381:1387	arg1	P100					1405:1408	P100	1405:1408	P100	1405:1408	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	90	theme	smaller	1381:1387	arg1	P45					1400:1402	P45	1400:1402	P45	1400:1402	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	90	theme	smaller	1381:1387	arg1	S5					1421:1422	S5	1421:1422	S5	1421:1422	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	90	theme	smaller	1381:1387	arg1	LMWH					1411:1414	LMWH	1411:1414	LMWH	1411:1414	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	90	theme	smaller	1381:1387	arg1	polyanions					1389:1398	The smaller polyanions P45, P100, LMWH, and S5	1377:1422	The smaller polyanions P45, P100, LMWH, and S5	1377:1422	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	8	91	theme	100	1331:1333	arg1	monomers					1356:1363	45 (P45), 100 (P100) or 700 (P700) monomers	1321:1363	45 (P45), 100 (P100) or 700 (P700) monomers at average	1321:1374	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	13	92	from	presence	2135:2142	arg1	phenomenon					2099:2108	a kringle-dependent phenomenon	2079:2108	a kringle-dependent phenomenon	2079:2108	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	92	from	presence	2135:2142	arg1	prolongation					2018:2029	The 20-35% prolongation	2007:2029	The 20-35% prolongation of lysis-times caused by all polyphosphates	2007:2073	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	92	from	presence	2135:2142	arg1	dampened					2119:2126	dampened	2119:2126	dampened	2119:2126	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	2	93	theme	NET	410:412	arg1	components					414:423	NET components	410:423	NET components	410:423	In thrombi the polyanionic DNA confers mechanical and lytic resistance to fibrin and heparins interfere with the effects of NET components.
31604180	0	94	theme	proteolytic	63:73	arg1	decomposition					75:87	mechanical and proteolytic decomposition	48:87	mechanical and proteolytic decomposition: Effects of polymer size and electric charge	48:132	Biorelevant polyanions stabilize fibrin against mechanical and proteolytic decomposition: Effects of polymer size and electric charge.
31604180	14	95	theme	plasmin-mediated	2388:2403	arg1	fibrinolysis					2405:2416	plasmin-mediated fibrinolysis	2388:2416	plasmin-mediated fibrinolysis	2388:2416	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	14	96	theme	chemical	2260:2267	arg1	structure					2269:2277	different chemical structure	2250:2277	different chemical structure	2250:2277	In summary, we found that polyanions of different chemical structure stabilize fibrin clots via size-dependent modulation of fibrin structure and kringle-dependent inhibition of plasmin-mediated fibrinolysis.
31604180	13	97	theme	lysis-times	2034:2044	arg1	phenomenon					2099:2108	a kringle-dependent phenomenon	2079:2108	a kringle-dependent phenomenon	2079:2108	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	97	theme	lysis-times	2034:2044	arg1	prolongation					2018:2029	The 20-35% prolongation	2007:2029	The 20-35% prolongation of lysis-times caused by all polyphosphates	2007:2073	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	97	theme	lysis-times	2034:2044	arg1	dampened					2119:2126	dampened	2119:2126	dampened	2119:2126	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	8	98	theme	fibrin	1115:1120	arg1	clots					1122:1126	composite fibrin clots	1105:1126	composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH)	1105:1265	Kinetic turbidimetric assays for the generation and dissolution of composite fibrin clots containing unfractionated heparin (UFH), and its partially or fully desulfated derivatives, as well as low molecular-weight heparin (LMWH), pentasaccharide (S5), and polyphosphates composed of 45 (P45), 100 (P100) or 700 (P700) monomers at average.
31604180	9	99	dep	polyanions	1389:1398	arg1	P100					1405:1408	P100	1405:1408	P100	1405:1408	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	99	dep	polyanions	1389:1398	arg1	P45					1400:1402	P45	1400:1402	P45	1400:1402	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	99	dep	polyanions	1389:1398	arg1	polyanions					1389:1398	The smaller polyanions P45, P100, LMWH, and S5	1377:1422	The smaller polyanions P45, P100, LMWH, and S5	1377:1422	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	99	dep	polyanions	1389:1398	arg1	LMWH					1411:1414	LMWH	1411:1414	LMWH	1411:1414	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	9	99	dep	polyanions	1389:1398	arg1	S5					1421:1422	S5	1421:1422	S5	1421:1422	The smaller polyanions P45, P100, LMWH, and S5 accelerated, whereas P700 and UFH retarded clot formation.
31604180	12	100	with	lysis-times	1933:1943	arg1	plasmin					1950:1956	plasmin	1950:1956	plasmin	1950:1956	Despite the size-dependent structural changes, all heparins caused a 10-15% prolongation of lysis-times with plasmin, and UFH-effects depended on sulfation patterns.
31604180	1	101	theme	essential	222:230	arg1	mechanism					232:240	an essential mechanism	219:240	an essential mechanism in the neutrophil-mediated innate immunity	219:283	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	1	101	theme	essential	222:230	arg1	release					139:145	The release	135:145	The release of neutrophil extracellular traps (NETs) containing DNA and histones	135:214	The release of neutrophil extracellular traps (NETs) containing DNA and histones is an essential mechanism in the neutrophil-mediated innate immunity.
31604180	13	102	theme	plasmin	2201:2207	arg1	plasmin					2201:2207	plasmin	2201:2207	plasmin	2201:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	13	102	theme	plasmin	2201:2207	arg1	sites					2192:2196	the lysine-binding sites	2173:2196	the lysine-binding sites of plasmin	2173:2207	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31604180	4	103	theme	polyanions	614:623	arg1	type					606:609	a different type	594:609	a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers)	594:698	Platelets and microorganisms release a different type of polyanions (polyphosphates) of various size (in the range 60-1000 phosphate monomers).
31604180	3	104	theme	mast	517:520	arg1	cells					522:526	mast cells	517:526	mast cells	517:526	Heparins are polyanions used not only as therapeutic agents, but they are also released by mast cells at entry sites of pathogens.
31604180	13	105	theme	20-35	2011:2015	arg1	%					2016:2016	%	2016:2016	%	2016:2016	The 20-35% prolongation of lysis-times caused by all polyphosphates was a kringle-dependent phenomenon, and was dampened in the presence of 6-aminohexanoate blocking the lysine-binding sites of plasmin.
31295490	2	0	theme	cellulose	376:384	arg1	nanofibrils					386:396	cellulose nanofibrils	376:396	cellulose nanofibrils	376:396	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	8	1	theme	high	1121:1124	arg1	biocompatibility					1126:1141	high biocompatibility	1121:1141	high biocompatibility	1121:1141	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	0	2	theme	coronary	112:119	arg1	cells					140:144	heart coronary artery endothelial cells	106:144	heart coronary artery endothelial cells	106:144	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	2	3	theme	Composite	278:286	arg1	carriers					296:303	Composite tubular carriers	278:303	Composite tubular carriers	278:303	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	5	4	theme	FT-IR	653:657	arg1	results					684:690	The FT-IR and chemical composition results	649:690	The FT-IR and chemical composition results	649:690	The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties and polysaccharides.
31295490	0	5	theme	heart	106:110	arg1	cells					140:144	heart coronary artery endothelial cells	106:144	heart coronary artery endothelial cells	106:144	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	8	6	contain	have	1147:1150	arg2	applications					1162:1173	potential applications	1152:1173	potential applications in heart tissue engineering	1152:1201	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	8	6	contain	have	1147:1150	arg1	carriers					1076:1083	tubular carriers	1068:1083	tubular carriers prepared from LCC/CNF composite	1068:1115	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	7	7	theme	control	955:961	arg1	groups					963:968	the LCC-48/CNF and control groups	936:968	groups	963:968	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	7	theme	control	955:961	arg1	rate					1027:1030	cell proliferation rate	1008:1030	cell proliferation rate	1008:1030	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	7	theme	control	955:961	arg1	activity					1046:1053	metabolic activity	1036:1053	metabolic activity	1036:1053	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	0	8	theme	endothelial	128:138	arg1	cells					140:144	heart coronary artery endothelial cells	106:144	heart coronary artery endothelial cells	106:144	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	2	9	theme	raw	408:410	arg1	materials					412:420	raw materials	408:420	raw materials	408:420	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	0	10	theme	artery	121:126	arg1	cells					140:144	heart coronary artery endothelial cells	106:144	heart coronary artery endothelial cells	106:144	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	6	11	theme	microscope	795:804	arg1	studies					806:812	The SEM and inverted microscope studies	774:812	studies	806:812	The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers.
31295490	5	12	theme	lignin	737:742	arg1	moieties					744:751	lignin moieties	737:751	lignin moieties	737:751	The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties and polysaccharides.
31295490	4	13	used	used	526:529	arg2	carriers					512:519	The carriers	508:519	The carriers	508:519	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	6	14	theme	inverted	786:793	arg1	studies					806:812	The SEM and inverted microscope studies	774:812	studies	806:812	The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers.
31295490	1	15	dep	LCC-48	183:188	arg1	i.e.					178:181	i.e.	178:181	i.e.	178:181	Lignin-carbohydrate complexes, i.e. LCC-48 and LCC-72 were isolated with vibrational ball milling for 48 h and 72 h, respectively.
31295490	3	16	dep	structures	427:436	arg1	The					423:425	The	423:425	The	423:425	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	3	17	theme	chemical	442:449	arg1	properties					451:460	chemical properties	442:460	chemical properties	442:460	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	4	18	theme	endothelial	564:574	arg1	cells					576:580	culture human coronary artery endothelial cells	534:580	culture human coronary artery endothelial cells (HCAEC)	534:588	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	4	18	theme	endothelial	564:574	arg1	HCAEC					583:587	HCAEC	583:587	HCAEC	583:587	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	2	19	theme	film	330:333	arg1	formation					335:343	film formation	330:343	film formation	330:343	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	3	20	theme	time	483:486	arg1	LCCs					488:491	different milling time LCCs	465:491	different milling time LCCs	465:491	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	0	21	theme	nanocellulose	15:27	arg1	culture					95:101	culture	95:101	culture	95:101	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	0	21	theme	nanocellulose	15:27	arg1	Preparation					0:10	Preparation	0:10	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers	0:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	0	22	theme	cells	140:144	arg1	culture					95:101	culture	95:101	culture	95:101	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	0	22	theme	cells	140:144	arg1	Preparation					0:10	Preparation	0:10	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers	0:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	6	23	theme	porous	867:872	arg1	carriers					874:881	the porous carriers	863:881	the porous carriers	863:881	The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers.
31295490	3	24	theme	LCCs	488:491	arg1	properties					451:460	chemical properties	442:460	chemical properties	442:460	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	3	24	theme	LCCs	488:491	arg1	structures					427:436	structures	427:436	structures	427:436	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	0	25	theme	complex	53:59	arg1	culture					95:101	culture	95:101	culture	95:101	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	0	25	theme	complex	53:59	arg1	Preparation					0:10	Preparation	0:10	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers	0:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	8	26	from	applications	1162:1173	arg1	engineering					1191:1201	heart tissue engineering	1178:1201	heart tissue engineering	1178:1201	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	3	27	theme	different	465:473	arg1	LCCs					488:491	different milling time LCCs	465:491	different milling time LCCs	465:491	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	0	28	theme	lignin-carbohydrate	33:51	arg1	complex					53:59	lignin-carbohydrate complex	33:59	lignin-carbohydrate complex	33:59	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	4	29	theme	human	542:546	arg1	cells					576:580	culture human coronary artery endothelial cells	534:580	culture human coronary artery endothelial cells (HCAEC)	534:588	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	4	29	theme	human	542:546	arg1	HCAEC					583:587	HCAEC	583:587	HCAEC	583:587	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	8	30	theme	LCC/CNF	1099:1105	arg1	composite					1107:1115	LCC/CNF composite	1099:1115	LCC/CNF composite	1099:1115	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	3	31	theme	milling	475:481	arg1	LCCs					488:491	different milling time LCCs	465:491	different milling time LCCs	465:491	The structures and chemical properties of different milling time LCCs were analyzed.
31295490	0	32	theme	biological	71:80	arg1	carriers					82:89	composite biological carriers	61:89	composite biological carriers	61:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	4	33	theme	culture	534:540	arg1	cells					576:580	culture human coronary artery endothelial cells	534:580	culture human coronary artery endothelial cells (HCAEC)	534:588	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	4	33	theme	culture	534:540	arg1	HCAEC					583:587	HCAEC	583:587	HCAEC	583:587	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	5	34	theme	chemical	663:670	arg1	composition					672:682	chemical composition	663:682	chemical composition	663:682	The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties and polysaccharides.
31295490	0	35	theme	composite	61:69	arg1	carriers					82:89	composite biological carriers	61:89	composite biological carriers	61:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	4	36	theme	artery	557:562	arg1	cells					576:580	culture human coronary artery endothelial cells	534:580	culture human coronary artery endothelial cells (HCAEC)	534:588	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	4	36	theme	artery	557:562	arg1	HCAEC					583:587	HCAEC	583:587	HCAEC	583:587	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	5	37	theme	composition	672:682	arg1	results					684:690	The FT-IR and chemical composition results	649:690	The FT-IR and chemical composition results	649:690	The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties and polysaccharides.
31295490	6	38	theme	cells	846:850	arg1	number					836:841	a large number	828:841	a large number of cells	828:850	The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers.
31295490	7	39	theme	cell	1008:1011	arg1	rate					1027:1030	cell proliferation rate	1008:1030	cell proliferation rate	1008:1030	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	8	40	theme	tubular	1068:1074	arg1	carriers					1076:1083	tubular carriers	1068:1083	tubular carriers prepared from LCC/CNF composite	1068:1115	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	7	41	theme	LCC-72/CNF	903:912	arg1	carriers					914:921	the LCC-72/CNF carriers	899:921	the LCC-72/CNF carriers	899:921	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	4	42	theme	coronary	548:555	arg1	cells					576:580	culture human coronary artery endothelial cells	534:580	culture human coronary artery endothelial cells (HCAEC)	534:588	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	4	42	theme	coronary	548:555	arg1	HCAEC					583:587	HCAEC	583:587	HCAEC	583:587	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	7	43	theme	metabolic	1036:1044	arg1	activity					1046:1053	metabolic activity	1036:1053	metabolic activity	1036:1053	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	44	theme	LCC-48/CNF	940:949	arg1	groups					963:968	the LCC-48/CNF and control groups	936:968	groups	963:968	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	44	theme	LCC-48/CNF	940:949	arg1	rate					1027:1030	cell proliferation rate	1008:1030	cell proliferation rate	1008:1030	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	44	theme	LCC-48/CNF	940:949	arg1	activity					1046:1053	metabolic activity	1036:1053	metabolic activity	1036:1053	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	2	45	theme	tubular	288:294	arg1	carriers					296:303	Composite tubular carriers	278:303	Composite tubular carriers	278:303	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	7	46	theme	proliferation	1013:1025	arg1	rate					1027:1030	cell proliferation rate	1008:1030	cell proliferation rate	1008:1030	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	1	47	theme	Lignin-carbohydrate	147:165	arg1	complexes					167:175	Lignin-carbohydrate complexes	147:175	Lignin-carbohydrate complexes	147:175	Lignin-carbohydrate complexes, i.e. LCC-48 and LCC-72 were isolated with vibrational ball milling for 48 h and 72 h, respectively.
31295490	2	48	dep	LCCs	367:370	arg1	the					363:365	the	363:365	the	363:365	Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials.
31295490	8	49	theme	tissue	1184:1189	arg1	engineering					1191:1201	heart tissue engineering	1178:1201	heart tissue engineering	1178:1201	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	0	50	dep	nanocellulose	15:27	arg1	carriers					82:89	composite biological carriers	61:89	composite biological carriers	61:89	Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
31295490	8	51	contain	had	1117:1119	arg2	biocompatibility					1126:1141	high biocompatibility	1121:1141	high biocompatibility	1121:1141	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	8	51	contain	had	1117:1119	arg1	carriers					1076:1083	tubular carriers	1068:1083	tubular carriers prepared from LCC/CNF composite	1068:1115	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	8	52	theme	potential	1152:1160	arg1	applications					1162:1173	potential applications	1152:1173	potential applications in heart tissue engineering	1152:1201	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31295490	6	53	theme	large	830:834	arg1	number					836:841	a large number	828:841	a large number of cells	828:850	The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers.
31295490	1	54	theme	vibrational	220:230	arg1	ball					232:235	vibrational ball	220:235	vibrational ball milling for 48 h and 72 h, respectively	220:275	Lignin-carbohydrate complexes, i.e. LCC-48 and LCC-72 were isolated with vibrational ball milling for 48 h and 72 h, respectively.
31295490	4	55	theme	cells	619:623	arg1	activities					599:608	the activities	595:608	the activities of these cells	595:623	The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro.
31295490	7	56	theme	observed	979:986	arg1	category					988:995	every observed category	973:995	every observed category	973:995	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	7	57	from	groups	963:968	arg1	category					988:995	every observed category	973:995	every observed category	973:995	HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity.
31295490	8	58	theme	heart	1178:1182	arg1	engineering					1191:1201	heart tissue engineering	1178:1201	heart tissue engineering	1178:1201	In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
31955743	2	0	dep	samples	565:571	arg1	TWC65					580:584	TWC65	580:584	TWC65	580:584	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	0	dep	samples	565:571	arg1	TWC50					573:577	TWC50	573:577	TWC50	573:577	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	0	dep	samples	565:571	arg1	TWC80					590:594	TWC80	590:594	TWC80	590:594	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	0	dep	samples	565:571	arg1	samples					565:571	the samples TWC50, TWC65 and TWC80	561:594	the samples TWC50, TWC65 and TWC80	561:594	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	8	1	theme	aggregates	1628:1637	arg1	adsorption					1590:1599	the effective adsorption	1576:1599	the effective adsorption of compact rich-in-protein aggregates at the air/water interface	1576:1664	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	8	1	theme	aggregates	1628:1637	arg1	content					1681:1687	the higher content	1670:1687	the higher content of soluble polysaccharides in the bulk phase	1670:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	5	2	theme	turbidity	1193:1201	arg1	pH-dependence					1176:1188	the pH-dependence	1172:1188	the pH-dependence of turbidity and solubility of protein and polysaccharides	1172:1247	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	5	3	theme	solubility	1207:1216	arg1	pH-dependence					1176:1188	the pH-dependence	1172:1188	the pH-dependence of turbidity and solubility of protein and polysaccharides	1172:1247	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	7	4	theme	carbohydrate-to-protein	1424:1446	arg1	ratio					1453:1457	the highest carbohydrate-to-protein mass ratio	1412:1457	the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8)	1412:1488	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	7	4	theme	carbohydrate-to-protein	1424:1446	arg1	>1.8					1484:1487	>1.8	1484:1487	>1.8	1484:1487	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	1	5	from	interface	300:308	arg1	medium					341:346	acid medium	336:346	acid medium	336:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	1	5	from	interface	300:308	arg1	behavior					274:281	their surface behavior	260:281	their surface behavior at the air/water interface and foaming properties in acid medium	260:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	9	6	theme	aerated	1856:1862	arg1	emulsions					1869:1877	aerated food emulsions	1856:1877	aerated food emulsions	1856:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	1	7	theme	composition	193:203	arg1	impact					174:179	the impact	170:179	the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium	170:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	0	8	theme	acid	133:136	arg1	medium					138:143	acid medium	133:143	acid medium	133:143	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	8	9	theme	soluble	1692:1698	arg1	polysaccharides					1700:1714	soluble polysaccharides	1692:1714	soluble polysaccharides	1692:1714	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	1	10	theme	structural	209:218	arg1	properties					220:229	structural properties	209:229	structural properties	209:229	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	4	11	theme	glycosylation	881:893	arg1	degree					871:876	the degree	867:876	the degree of glycosylation	867:893	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	5	12	contain	have	1144:1147	arg1	differences					1132:1142	These structural differences	1115:1142	These structural differences	1115:1142	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	5	12	contain	have	1144:1147	arg2	impact					1162:1167	a pronounced impact	1149:1167	a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides	1149:1247	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	8	13	theme	bulk	1723:1726	arg1	phase					1728:1732	the bulk phase	1719:1732	the bulk phase	1719:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	3	14	theme	polypeptide	818:828	arg1	composition					830:840	the polypeptide composition	814:840	the polypeptide composition	814:840	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	2	15	theme	further	505:511	arg1	dialysis					513:520	further dialysis	505:520	further dialysis (4 °C, 48 h)	505:533	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	1	16	theme	acid	336:339	arg1	medium					341:346	acid medium	336:346	acid medium	336:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	4	17	theme	molecular	964:972	arg1	flexibility					974:984	molecular flexibility	964:984	molecular flexibility	964:984	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	1	18	dep	interface	300:308	arg1	the					286:288	the	286:288	the	286:288	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	4	19	theme	hydrophobic	920:930	arg1	effect					932:937	the hydrophobic effect	916:937	the hydrophobic effect	916:937	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	0	20	from	Influence	0:8	arg1	behavior					75:82	the surface behavior and foam properties	63:102	behavior	75:82	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	0	20	from	Influence	0:8	arg1	properties					93:102	the surface behavior and foam properties	63:102	properties	93:102	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	3	21	from	changes	763:769	arg1	yield					778:782	the yield	774:782	the yield	774:782	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	8	22	from	adsorption	1590:1599	arg1	phase					1728:1732	the bulk phase	1719:1732	the bulk phase	1719:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	3	23	theme	carbohydrate	789:800	arg1	content					802:808	the carbohydrate content	785:808	the carbohydrate content	785:808	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	0	24	theme	foam	88:91	arg1	properties					93:102	the surface behavior and foam properties	63:102	properties	93:102	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	6	25	theme	foam	1337:1340	arg1	ability					1342:1348	their foam ability	1331:1348	their foam ability	1331:1348	At pH 4.0 all concentrates evidenced a ζ-potential close to zero, which enhanced their foam ability (overrun >1500%).
31955743	4	26	dep	increase	858:865	arg1	degree					871:876	the degree	867:876	the degree of glycosylation	867:893	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	4	27	theme	compact	1007:1013	arg1	aggregates					1015:1024	compact aggregates	1007:1024	compact aggregates mediated by disulfide bridges	1007:1054	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	2	28	theme	pressure	439:446	arg1	combinations					407:418	three combinations	401:418	three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa)	401:497	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	29	theme	temperature	423:433	arg1	combinations					407:418	three combinations	401:418	three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa)	401:497	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	0	30	theme	chemical	13:20	arg1	composition					22:32	chemical composition	13:32	chemical composition	13:32	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	1	31	theme	air/water	290:298	arg1	interface					300:308	air/water interface	290:308	air/water interface	290:308	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	2	32	dep	dialysis	513:520	arg1	48 h					529:532	48 h	529:532	48 h	529:532	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	32	dep	dialysis	513:520	arg1	4 °C					523:526	4 °C	523:526	4 °C	523:526	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	7	33	theme	foam	1494:1497	arg1	FS					1510:1511	FS	1510:1511	FS	1510:1511	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	7	33	theme	foam	1494:1497	arg1	stability					1499:1507	foam stability	1494:1507	foam stability (FS)	1494:1512	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	3	34	theme	temperature	627:637	arg1	increase					615:622	The increase	611:622	The increase of temperature during the concentration step	611:667	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	8	35	from	interface	1656:1664	arg1	adsorption					1590:1599	the effective adsorption	1576:1599	the effective adsorption of compact rich-in-protein aggregates at the air/water interface	1576:1664	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	8	35	from	interface	1656:1664	arg1	content					1681:1687	the higher content	1670:1687	the higher content of soluble polysaccharides in the bulk phase	1670:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	2	36	theme	Liquid	349:354	arg1	pH					367:368	pH 5.6 ± 0.1	367:378	pH 5.6 ± 0.1	367:378	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	36	theme	Liquid	349:354	arg1	tofu-whey					356:364	Liquid tofu-whey	349:364	Liquid tofu-whey (pH 5.6 ± 0.1)	349:379	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	0	37	theme	properties	49:58	arg1	Influence					0:8	Influence	0:8	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey	0:115	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	9	38	theme	tofu-whey	1786:1794	arg1	concentrates					1796:1807	tofu-whey concentrates	1786:1807	tofu-whey concentrates	1786:1807	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	8	39	from	content	1681:1687	arg1	phase					1728:1732	the bulk phase	1719:1732	the bulk phase	1719:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	1	40	theme	properties	220:229	arg1	impact					174:179	the impact	170:179	the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium	170:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	7	41	theme	soluble	1466:1472	arg1	fraction					1474:1481	the soluble fraction	1462:1481	the soluble fraction	1462:1481	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	8	42	theme	effective	1580:1588	arg1	adsorption					1590:1599	the effective adsorption	1576:1599	the effective adsorption of compact rich-in-protein aggregates at the air/water interface	1576:1664	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	1	43	theme	tofu-whey	234:242	arg1	concentrates					244:255	tofu-whey concentrates	234:255	tofu-whey concentrates	234:255	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	9	44	from	application	1771:1781	arg1	systems					1829:1835	acidic dispersed systems	1812:1835	acidic dispersed systems	1812:1835	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	44	from	application	1771:1781	arg1	foams					1846:1850	foams	1846:1850	foams	1846:1850	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	44	from	application	1771:1781	arg1	emulsions					1869:1877	aerated food emulsions	1856:1877	aerated food emulsions	1856:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	45	theme	dispersed	1819:1827	arg1	systems					1829:1835	acidic dispersed systems	1812:1835	acidic dispersed systems	1812:1835	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	45	theme	dispersed	1819:1827	arg1	foams					1846:1850	foams	1846:1850	foams	1846:1850	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	45	theme	dispersed	1819:1827	arg1	emulsions					1869:1877	aerated food emulsions	1856:1877	aerated food emulsions	1856:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	1	46	from	properties	322:331	arg1	medium					341:346	acid medium	336:346	acid medium	336:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	1	46	from	properties	322:331	arg1	behavior					274:281	their surface behavior	260:281	their surface behavior at the air/water interface and foaming properties in acid medium	260:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	7	47	theme	mass	1448:1451	arg1	ratio					1453:1457	the highest carbohydrate-to-protein mass ratio	1412:1457	the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8)	1412:1488	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	7	47	theme	mass	1448:1451	arg1	>1.8					1484:1487	>1.8	1484:1487	>1.8	1484:1487	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	8	48	theme	rich-in-protein	1612:1626	arg1	aggregates					1628:1637	compact rich-in-protein aggregates	1604:1637	compact rich-in-protein aggregates	1604:1637	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	1	49	theme	surface	266:272	arg1	behavior					274:281	their surface behavior	260:281	their surface behavior at the air/water interface and foaming properties in acid medium	260:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	7	50	theme	highest	1416:1422	arg1	ratio					1453:1457	the highest carbohydrate-to-protein mass ratio	1412:1457	the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8)	1412:1488	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	7	50	theme	highest	1416:1422	arg1	>1.8					1484:1487	>1.8	1484:1487	>1.8	1484:1487	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	3	51	theme	crude	716:720	arg1	protein					722:728	crude protein	716:728	crude protein	716:728	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	7	52	from	stability	1499:1507	arg1	fraction					1474:1481	the soluble fraction	1462:1481	the soluble fraction	1462:1481	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	3	53	theme	concentrates	700:711	arg1	enrichment					682:691	the enrichment	678:691	the enrichment of the concentrates in crude protein and calcium	678:740	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	8	54	theme	air/water	1646:1654	arg1	interface					1656:1664	the air/water interface	1642:1664	the air/water interface	1642:1664	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	9	55	theme	food	1864:1867	arg1	emulsions					1869:1877	aerated food emulsions	1856:1877	aerated food emulsions	1856:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	8	56	from	pH	1546:1547	arg1	improvement					1525:1535	the improvement	1521:1535	the improvement of FS at pH 4.0	1521:1551	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	1	57	theme	chemical	184:191	arg1	composition					193:203	chemical composition	184:203	chemical composition	184:203	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	4	58	theme	FTIR	1102:1105	arg1	assays					1107:1112	FTIR assays	1102:1112	FTIR assays	1102:1112	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	8	59	theme	higher	1674:1679	arg1	content					1681:1687	the higher content	1670:1687	the higher content of soluble polysaccharides in the bulk phase	1670:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	7	60	from	ratio	1453:1457	arg1	fraction					1474:1481	the soluble fraction	1462:1481	the soluble fraction	1462:1481	Nevertheless, at this pH, TWC80 showed both the highest carbohydrate-to-protein mass ratio in the soluble fraction (>1.8) and foam stability (FS).
31955743	3	61	from	enrichment	682:691	arg1	calcium					734:740	calcium	734:740	calcium	734:740	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	3	61	from	enrichment	682:691	arg1	protein					722:728	crude protein	716:728	crude protein	716:728	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	6	62	dep	enhanced	1322:1329	arg1	%					1364:1364	overrun >1500%	1351:1364	overrun >1500%	1351:1364	At pH 4.0 all concentrates evidenced a ζ-potential close to zero, which enhanced their foam ability (overrun >1500%).
31955743	3	63	theme	appreciable	751:761	arg1	changes					763:769	appreciable changes	751:769	appreciable changes in the yield	751:782	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	8	64	theme	polysaccharides	1700:1714	arg1	adsorption					1590:1599	the effective adsorption	1576:1599	the effective adsorption of compact rich-in-protein aggregates at the air/water interface	1576:1664	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	8	64	theme	polysaccharides	1700:1714	arg1	content					1681:1687	the higher content	1670:1687	the higher content of soluble polysaccharides in the bulk phase	1670:1732	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	0	65	theme	surface	67:73	arg1	behavior					75:82	the surface behavior and foam properties	63:102	behavior	75:82	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	6	66	theme	overrun	1351:1357	arg1	%					1364:1364	overrun >1500%	1351:1364	overrun >1500%	1351:1364	At pH 4.0 all concentrates evidenced a ζ-potential close to zero, which enhanced their foam ability (overrun >1500%).
31955743	5	67	theme	structural	1121:1130	arg1	differences					1132:1142	These structural differences	1115:1142	These structural differences	1115:1142	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	0	68	theme	tofu-whey	107:115	arg1	behavior					75:82	the surface behavior and foam properties	63:102	behavior	75:82	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	0	68	theme	tofu-whey	107:115	arg1	properties					93:102	the surface behavior and foam properties	63:102	properties	93:102	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	2	69	dep	pressure	439:446	arg1	65 °C-16.0 kPa					464:477	65 °C-16.0 kPa	464:477	65 °C-16.0 kPa	464:477	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	69	dep	pressure	439:446	arg1	50 °C-8.0 kPa					449:461	50 °C-8.0 kPa	449:461	50 °C-8.0 kPa	449:461	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	2	69	dep	pressure	439:446	arg1	80 °C-24.0 kPa					483:496	80 °C-24.0 kPa	483:496	80 °C-24.0 kPa	483:496	Liquid tofu-whey (pH 5.6 ± 0.1) was concentrated at three combinations of temperature and pressure (50 °C-8.0 kPa, 65 °C-16.0 kPa and 80 °C-24.0 kPa), with further dialysis (4 °C, 48 h) and freeze-drying, giving the samples TWC50, TWC65 and TWC80, respectively.
31955743	4	70	theme	effect	932:937	arg1	intensity					903:911	the intensity	899:911	the intensity of the hydrophobic effect	899:937	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	4	70	theme	effect	932:937	arg1	increase					858:865	the increase the degree of glycosylation and the intensity of the hydrophobic effect	854:937	increase	858:865	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	0	71	theme	composition	22:32	arg1	Influence					0:8	Influence	0:8	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey	0:115	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	4	72	theme	aggregates	1015:1024	arg1	decrease					952:959	the decrease	948:959	the decrease of molecular flexibility	948:984	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	4	72	theme	aggregates	1015:1024	arg1	formation					994:1002	the formation	990:1002	the formation of compact aggregates mediated by disulfide bridges	990:1054	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	0	73	theme	structural	38:47	arg1	properties					49:58	structural properties	38:58	structural properties	38:58	Influence of chemical composition and structural properties on the surface behavior and foam properties of tofu-whey concentrates in acid medium.
31955743	5	74	theme	pH-dependence	1176:1188	arg1	impact					1162:1167	a pronounced impact	1149:1167	a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides	1149:1247	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	4	75	theme	flexibility	974:984	arg1	decrease					952:959	the decrease	948:959	the decrease of molecular flexibility	948:984	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	4	75	theme	flexibility	974:984	arg1	formation					994:1002	the formation	990:1002	the formation of compact aggregates mediated by disulfide bridges	990:1054	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	1	76	theme	foaming	314:320	arg1	properties					322:331	foaming properties	314:331	foaming properties	314:331	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	1	77	from	impact	174:179	arg1	behavior					274:281	their surface behavior	260:281	their surface behavior at the air/water interface and foaming properties in acid medium	260:346	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	3	78	theme	concentration	650:662	arg1	step					664:667	the concentration step	646:667	the concentration step	646:667	The increase of temperature during the concentration step promoted the enrichment of the concentrates in crude protein and calcium, without appreciable changes in the yield, the carbohydrate content and the polypeptide composition.
31955743	5	79	theme	pronounced	1151:1160	arg1	impact					1162:1167	a pronounced impact	1149:1167	a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides	1149:1247	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	9	80	theme	concentrates	1796:1807	arg1	application					1771:1781	the application	1767:1781	the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions	1767:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	8	81	theme	FS	1540:1541	arg1	improvement					1525:1535	the improvement	1521:1535	the improvement of FS at pH 4.0	1521:1551	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	5	82	theme	protein	1221:1227	arg1	solubility					1207:1216	solubility	1207:1216	solubility	1207:1216	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	5	82	theme	protein	1221:1227	arg1	turbidity					1193:1201	turbidity	1193:1201	turbidity	1193:1201	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	4	83	theme	disulfide	1038:1046	arg1	bridges					1048:1054	disulfide bridges	1038:1054	disulfide bridges	1038:1054	For TWC80, the increase the degree of glycosylation and the intensity of the hydrophobic effect promoted the decrease of molecular flexibility and the formation of compact aggregates mediated by disulfide bridges as was evidenced by tricine-SDS-PAGE, TGA and FTIR assays.
31955743	9	84	theme	acidic	1812:1817	arg1	systems					1829:1835	acidic dispersed systems	1812:1835	acidic dispersed systems	1812:1835	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	84	theme	acidic	1812:1817	arg1	foams					1846:1850	foams	1846:1850	foams	1846:1850	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	9	84	theme	acidic	1812:1817	arg1	emulsions					1869:1877	aerated food emulsions	1856:1877	aerated food emulsions	1856:1877	These findings are relevant for the application of tofu-whey concentrates in acidic dispersed systems, such as foams and aerated food emulsions.
31955743	1	85	theme	concentrates	244:255	arg1	composition					193:203	chemical composition	184:203	chemical composition	184:203	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	1	85	theme	concentrates	244:255	arg1	properties					220:229	structural properties	209:229	structural properties	209:229	This article focuses on the impact of chemical composition and structural properties of tofu-whey concentrates on their surface behavior at the air/water interface and foaming properties in acid medium.
31955743	8	86	theme	compact	1604:1610	arg1	aggregates					1628:1637	compact rich-in-protein aggregates	1604:1637	compact rich-in-protein aggregates	1604:1637	Thus, the improvement of FS at pH 4.0 would be associated to the effective adsorption of compact rich-in-protein aggregates at the air/water interface and the higher content of soluble polysaccharides in the bulk phase.
31955743	5	87	theme	polysaccharides	1233:1247	arg1	solubility					1207:1216	solubility	1207:1216	solubility	1207:1216	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
31955743	5	87	theme	polysaccharides	1233:1247	arg1	turbidity					1193:1201	turbidity	1193:1201	turbidity	1193:1201	These structural differences have a pronounced impact of the pH-dependence of turbidity and solubility of protein and polysaccharides.
30690433	0	0	theme	enhanced	107:114	arg1	healing					122:128	enhanced wound healing	107:128	enhanced wound healing	107:128	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
30690433	4	1	theme	controlled	516:525	arg1	delivery					527:534	controlled delivery	516:534	controlled delivery of growth factors to the wound site using nanoparticles	516:590	In addition, controlled delivery of growth factors to the wound site using nanoparticles can significantly improve the healing process.
30690433	5	2	theme	CNC-HA/GM-CSF-Chi-NPs	798:818	arg1	GM-CSF					790:795	GM-CSF	790:795	GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite)	790:829	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	2	theme	CNC-HA/GM-CSF-Chi-NPs	798:818	arg1	composite					820:828	CNC-HA/GM-CSF-Chi-NPs composite	798:828	CNC-HA/GM-CSF-Chi-NPs composite	798:828	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	8	3	theme	treated	1643:1649	arg1	wound					1651:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	6	4	theme	GM-CSF	1065:1070	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	4	theme	GM-CSF	1065:1070	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	4	theme	GM-CSF	1065:1070	arg1	properties					970:979	appropriate mechanical properties	947:979	appropriate mechanical properties	947:979	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	4	theme	GM-CSF	1065:1070	arg1	release					1054:1060	controlled release	1043:1060	controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing	1043:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	4	5	theme	growth	539:544	arg1	factors					546:552	growth factors	539:552	growth factors	539:552	In addition, controlled delivery of growth factors to the wound site using nanoparticles can significantly improve the healing process.
30690433	6	6	theme	CNC-HA/GM-CSF-Chi-NPs	863:883	arg1	composite					885:893	CNC-HA/GM-CSF-Chi-NPs composite	863:893	CNC-HA/GM-CSF-Chi-NPs composite	863:893	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	8	7	theme	enhanced	1532:1539	arg1	re-epithelialization					1541:1560	enhanced re-epithelialization	1532:1560	enhanced re-epithelialization	1532:1560	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	6	8	theme	appropriate	947:957	arg1	properties					970:979	appropriate mechanical properties	947:979	appropriate mechanical properties	947:979	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	3	9	theme	reinforcing	375:385	arg1	cellulose					418:426	nanocrystalline cellulose	402:426	nanocrystalline cellulose (CNC)	402:432	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	3	9	theme	reinforcing	375:385	arg1	agents					387:392	reinforcing agents	375:392	reinforcing agents such as nanocrystalline cellulose (CNC)	375:432	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	6	10	theme	swelling	987:994	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	10	theme	swelling	987:994	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	8	11	theme	improved	1566:1573	arg1	formation					1594:1602	improved granulation tissue formation	1566:1602	improved granulation tissue formation	1566:1602	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	4	12	theme	healing	622:628	arg1	process					630:636	the healing process	618:636	the healing process	618:636	In addition, controlled delivery of growth factors to the wound site using nanoparticles can significantly improve the healing process.
30690433	3	13	theme	agents	387:392	arg1	Incorporation					358:370	Incorporation	358:370	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings	358:460	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	6	14	theme	high	982:985	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	14	theme	high	982:985	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	9	15	theme	CNC-HA/GM-CSF-Chi-NPs	1759:1779	arg1	composite					1781:1789	CNC-HA/GM-CSF-Chi-NPs composite	1759:1789	CNC-HA/GM-CSF-Chi-NPs composite	1759:1789	Our results suggest that CNC-HA/GM-CSF-Chi-NPs composite can be potentially applied in clinical practice for wound treatment.
30690433	9	16	theme	clinical	1821:1828	arg1	practice					1830:1837	clinical practice	1821:1837	clinical practice for wound treatment	1821:1857	Our results suggest that CNC-HA/GM-CSF-Chi-NPs composite can be potentially applied in clinical practice for wound treatment.
30690433	6	17	theme	controlled	1043:1052	arg1	release					1054:1060	controlled release	1043:1060	controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing	1043:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	7	18	theme	CNC-HA/GM-CSF-Chi-NPs	1211:1231	arg1	composite					1233:1241	CNC-HA/GM-CSF-Chi-NPs composite	1211:1241	CNC-HA/GM-CSF-Chi-NPs composite	1211:1241	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	8	19	theme	composite	1633:1641	arg1	wound					1651:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	20	theme	In	1140:1141	arg1	investigation					1148:1160	In vivo investigation	1140:1160	In vivo investigation	1140:1160	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	5	21	theme	wound	847:851	arg1	dressing					853:860	an effective wound dressing	834:860	an effective wound dressing	834:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	21	theme	wound	847:851	arg1	nanoparticles					764:776	chitosan nanoparticles	755:776	chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite)	755:829	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	2	22	theme	mechanical	273:282	arg1	properties					284:293	the poor mechanical properties	264:293	the poor mechanical properties of HA-based wound dressings	264:321	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	1	23	theme	hyaluronic	184:193	arg1	HA					201:202	HA	201:202	HA	201:202	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	1	23	theme	hyaluronic	184:193	arg1	acid					195:198	hyaluronic acid	184:198	hyaluronic acid (HA) for wound dressing	184:222	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	6	24	dep	h	1081:1081	arg1	up					1072:1073	up	1072:1073	up	1072:1073	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	8	25	from	effects	1686:1692	arg1	process					1725:1731	the wound healing process	1707:1731	the wound healing process	1707:1731	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	2	26	theme	poor	268:271	arg1	properties					284:293	the poor mechanical properties	264:293	the poor mechanical properties of HA-based wound dressings	264:321	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	8	27	theme	positive	1677:1684	arg1	effects					1686:1692	positive effects	1677:1692	positive effects of GM-CSF on the wound healing process	1677:1731	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	3	28	theme	nanocrystalline	402:416	arg1	CNC					429:431	CNC	429:431	CNC	429:431	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	3	28	theme	nanocrystalline	402:416	arg1	cellulose					418:426	nanocrystalline cellulose	402:426	nanocrystalline cellulose (CNC)	402:432	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	6	29	theme	mechanical	959:968	arg1	properties					970:979	appropriate mechanical properties	947:979	appropriate mechanical properties	947:979	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	3	30	theme	HA-based	437:444	arg1	dressings					452:460	HA-based wound dressings	437:460	HA-based wound dressings	437:460	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	0	31	theme	Nanocrystalline	0:14	arg1	composite					42:50	Nanocrystalline cellulose-hyaluronic acid composite	0:50	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF	0:71	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
30690433	5	32	contain	containing	744:753	arg2	dressing					853:860	an effective wound dressing	834:860	an effective wound dressing	834:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	32	contain	containing	744:753	arg1	composite					734:742	a novel CNC reinforced HA-based composite	702:742	a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing	702:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	32	contain	containing	744:753	arg2	nanoparticles					764:776	chitosan nanoparticles	755:776	chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite)	755:829	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	2	33	theme	dressings	313:321	arg1	properties					284:293	the poor mechanical properties	264:293	the poor mechanical properties of HA-based wound dressings	264:321	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	5	34	theme	chitosan	755:762	arg1	dressing					853:860	an effective wound dressing	834:860	an effective wound dressing	834:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	34	theme	chitosan	755:762	arg1	nanoparticles					764:776	chitosan nanoparticles	755:776	chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite)	755:829	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	3	35	theme	wound	446:450	arg1	dressings					452:460	HA-based wound dressings	437:460	HA-based wound dressings	437:460	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	5	36	theme	reinforced	714:723	arg1	composite					734:742	a novel CNC reinforced HA-based composite	702:742	a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing	702:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	8	37	theme	treated	1464:1470	arg1	wounds					1472:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	2	38	theme	wound	307:311	arg1	dressings					313:321	HA-based wound dressings	298:321	HA-based wound dressings	298:321	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	3	39	theme	mechanical	480:489	arg1	properties					491:500	their mechanical properties	474:500	their mechanical properties	474:500	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	0	40	theme	acid	37:40	arg1	composite					42:50	Nanocrystalline cellulose-hyaluronic acid composite	0:50	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF	0:71	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
30690433	5	41	theme	HA-based	725:732	arg1	composite					734:742	a novel CNC reinforced HA-based composite	702:742	a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing	702:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	3	42	from	Incorporation	358:370	arg1	dressings					452:460	HA-based wound dressings	437:460	HA-based wound dressings	437:460	Incorporation of reinforcing agents such as nanocrystalline cellulose (CNC) in HA-based wound dressings can improve their mechanical properties.
30690433	2	43	theme	HA-based	298:305	arg1	dressings					313:321	HA-based wound dressings	298:321	HA-based wound dressings	298:321	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	5	44	theme	composite	734:742	arg1	development					666:676	development	666:676	development	666:676	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	44	theme	composite	734:742	arg1	characterization					682:697	characterization	682:697	characterization	682:697	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	7	45	theme	saline	1341:1346	arg1	wounds					1356:1361	the normal saline treated wounds	1330:1361	the normal saline treated wounds which exhibited nearly 70% of wound size reduction	1330:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	8	46	theme	composite	1454:1462	arg1	wounds					1472:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	6	47	theme	excellent	1100:1108	arg1	candidate					1110:1118	an excellent candidate	1097:1118	an excellent candidate for wound dressing	1097:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	1	48	theme	wound	209:213	arg1	dressing					215:222	wound dressing	209:222	wound dressing	209:222	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	6	49	theme	wound	1124:1128	arg1	dressing					1130:1137	wound dressing	1124:1137	wound dressing	1124:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	50	theme	%	1028:1028	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	50	theme	%	1028:1028	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	8	51	theme	healing	1717:1723	arg1	process					1725:1731	the wound healing process	1707:1731	the wound healing process	1707:1731	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	8	52	theme	wound	1711:1715	arg1	healing					1717:1723	wound healing	1711:1723	the wound healing process	1707:1731	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	53	theme	treated	1348:1354	arg1	wounds					1356:1361	the normal saline treated wounds	1330:1361	the normal saline treated wounds which exhibited nearly 70% of wound size reduction	1330:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	7	54	dep	In	1140:1141	arg1	vivo					1143:1146	vivo	1143:1146	vivo	1143:1146	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	4	55	theme	factors	546:552	arg1	delivery					527:534	controlled delivery	516:534	controlled delivery of growth factors to the wound site using nanoparticles	516:590	In addition, controlled delivery of growth factors to the wound site using nanoparticles can significantly improve the healing process.
30690433	7	56	theme	wound	1270:1274	arg1	closure					1276:1282	nearly full wound closure	1258:1282	nearly full wound closure	1258:1282	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	1	57	theme	recent	134:139	arg1	years					141:145	recent years	134:145	recent years	134:145	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	5	58	theme	effective	837:845	arg1	dressing					853:860	an effective wound dressing	834:860	an effective wound dressing	834:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	5	58	theme	effective	837:845	arg1	nanoparticles					764:776	chitosan nanoparticles	755:776	chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite)	755:829	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	4	59	theme	wound	561:565	arg1	site					567:570	the wound site	557:570	the wound site using nanoparticles	557:590	In addition, controlled delivery of growth factors to the wound site using nanoparticles can significantly improve the healing process.
30690433	9	60	theme	wound	1843:1847	arg1	treatment					1849:1857	wound treatment	1843:1857	wound treatment	1843:1857	Our results suggest that CNC-HA/GM-CSF-Chi-NPs composite can be potentially applied in clinical practice for wound treatment.
30690433	6	61	theme	physicochemical	907:921	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	61	theme	physicochemical	907:921	arg1	properties					970:979	appropriate mechanical properties	947:979	appropriate mechanical properties	947:979	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	61	theme	physicochemical	907:921	arg1	characteristics					923:937	some physicochemical characteristics	902:937	some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing	902:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	61	theme	physicochemical	907:921	arg1	release					1054:1060	controlled release	1043:1060	controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing	1043:1137	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	8	62	theme	lower	1503:1507	arg1	reaction					1522:1529	significantly lower inflammatory reaction	1489:1529	significantly lower inflammatory reaction	1489:1529	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	63	theme	normal	1334:1339	arg1	wounds					1356:1361	the normal saline treated wounds	1330:1361	the normal saline treated wounds which exhibited nearly 70% of wound size reduction	1330:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	6	64	theme	ratio	1015:1019	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	64	theme	ratio	1015:1019	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	5	65	theme	novel	704:708	arg1	composite					734:742	a novel CNC reinforced HA-based composite	702:742	a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing	702:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	7	66	theme	size	1399:1402	arg1	reduction					1404:1412	wound size reduction	1393:1412	wound size reduction	1393:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	8	67	theme	CNC-HA/GM-CSF-Chi-NPs	1432:1452	arg1	wounds					1472:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	the CNC-HA/GM-CSF-Chi-NPs composite treated wounds	1428:1477	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	2	68	theme	clinical	335:342	arg1	applications					344:355	their clinical applications	329:355	their clinical applications	329:355	However, the poor mechanical properties of HA-based wound dressings limit their clinical applications.
30690433	5	69	theme	CNC	710:712	arg1	composite					734:742	a novel CNC reinforced HA-based composite	702:742	a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing	702:860	In this study, we focus on development and characterization of a novel CNC reinforced HA-based composite containing chitosan nanoparticles loaded with GM-CSF (CNC-HA/GM-CSF-Chi-NPs composite) as an effective wound dressing.
30690433	8	70	theme	tissue	1587:1592	arg1	formation					1594:1602	improved granulation tissue formation	1566:1602	improved granulation tissue formation	1566:1602	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	71	theme	reduction	1404:1412	arg1	reduction					1404:1412	wound size reduction	1393:1412	wound size reduction	1393:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	7	71	theme	reduction	1404:1412	arg1	%					1388:1388	nearly 70%	1379:1388	nearly 70% of wound size reduction	1379:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	1	72	theme	biopolymers	164:174	arg1	applications					148:159	applications	148:159	applications of biopolymers such as hyaluronic acid (HA) for wound dressing	148:222	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	8	73	theme	GM-CSF	1697:1702	arg1	effects					1686:1692	positive effects	1677:1692	positive effects of GM-CSF on the wound healing process	1677:1731	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	74	theme	full	1265:1268	arg1	closure					1276:1282	nearly full wound closure	1258:1282	nearly full wound closure	1258:1282	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	8	75	theme	granulation	1575:1585	arg1	formation					1594:1602	improved granulation tissue formation	1566:1602	improved granulation tissue formation	1566:1602	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	8	76	theme	inflammatory	1509:1520	arg1	reaction					1522:1529	significantly lower inflammatory reaction	1489:1529	significantly lower inflammatory reaction	1489:1529	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	7	77	theme	complete	1288:1295	arg1	re-epithelialization					1297:1316	complete re-epithelialization	1288:1316	complete re-epithelialization	1288:1316	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	1	78	theme	more	239:242	arg1	attention					244:252	more attention	239:252	more attention	239:252	In recent years, applications of biopolymers such as hyaluronic acid (HA) for wound dressing have attracted more attention.
30690433	0	79	theme	chitosan	80:87	arg1	nanoparticles					89:101	chitosan nanoparticles	80:101	chitosan nanoparticles for enhanced wound healing	80:128	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
30690433	6	80	theme	swelling	1006:1013	arg1	%					1036:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	swelling ratio: 2622.1% ± 35.2%	1006:1036	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	6	80	theme	swelling	1006:1013	arg1	capacity					996:1003	high swelling capacity	982:1003	high swelling capacity (swelling ratio: 2622.1% ± 35.2%)	982:1037	CNC-HA/GM-CSF-Chi-NPs composite showed some physicochemical characteristics such as appropriate mechanical properties, high swelling capacity (swelling ratio: 2622.1% ± 35.2%) and controlled release of GM-CSF up to 48 h which make it an excellent candidate for wound dressing.
30690433	7	81	theme	wound	1393:1397	arg1	reduction					1404:1412	wound size reduction	1393:1412	wound size reduction	1393:1412	In vivo investigation showed that, after 13 d, the wounds covered with CNC-HA/GM-CSF-Chi-NPs composite could reach to nearly full wound closure and complete re-epithelialization compared to the normal saline treated wounds which exhibited nearly 70% of wound size reduction.
30690433	0	82	theme	cellulose-hyaluronic	16:35	arg1	composite					42:50	Nanocrystalline cellulose-hyaluronic acid composite	0:50	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF	0:71	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
30690433	8	83	theme	CNC-HA/Chi-NPs	1618:1631	arg1	wound					1651:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	CNC-HA/Chi-NPs composite treated wound	1618:1655	Furthermore, the CNC-HA/GM-CSF-Chi-NPs composite treated wounds exhibited significantly lower inflammatory reaction, enhanced re-epithelialization and improved granulation tissue formation compared with CNC-HA/Chi-NPs composite treated wound; it might be due to positive effects of GM-CSF on the wound healing process.
30690433	0	84	theme	wound	116:120	arg1	healing					122:128	enhanced wound healing	107:128	enhanced wound healing	107:128	Nanocrystalline cellulose-hyaluronic acid composite enriched with GM-CSF loaded chitosan nanoparticles for enhanced wound healing.
31547957	0	0	theme	sulfate	81:87	arg1	separation					59:68	the microscale chromatographic separation	28:68	the microscale chromatographic separation of heparan sulfate	28:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	9	1	from	origin	1311:1316	arg1	analysis					1251:1258	the analysis	1247:1258	the analysis of heparan sulfate samples from porcine and bovine origin	1247:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	9	1	from	origin	1311:1316	arg1	samples					1279:1285	heparan sulfate samples	1263:1285	heparan sulfate samples from porcine and bovine origin	1263:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	7	2	theme	individual	1024:1033	arg1	disaccharides					1035:1047	the individual disaccharides	1020:1047	the individual disaccharides	1020:1047	The LOD of the resulting method was 0.8-1.5 fmol for the individual disaccharides and the LOQ was between 2.5-5 fmol.
31547957	1	3	theme	real	158:161	arg1	challenge					163:171	a real challenge	156:171	a real challenge	156:171	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	5	4	theme	gradient	786:793	arg1	method					795:800	a salt gradient method	779:800	a salt gradient method in order to maximize separation efficiency	779:843	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	0	5	theme	heparan	73:79	arg1	sulfate					81:87	heparan sulfate	73:87	heparan sulfate	73:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	4	6	theme	%	545:545	arg1	acetonitrile					547:558	75% acetonitrile	543:558	75% acetonitrile	543:558	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	7	theme	bovine	1304:1309	arg1	origin					1311:1316	bovine origin	1304:1316	bovine origin	1304:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	3	8	theme	composition	374:384	arg1	effects					355:361	the chromatographic effects	335:361	the chromatographic effects of solvent composition, salt concentration, and salt type	335:419	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	0	9	from	Salt	0:3	arg1	separation					59:68	the microscale chromatographic separation	28:68	the microscale chromatographic separation of heparan sulfate	28:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	7	10	theme	method	992:997	arg1	LOD					971:973	The LOD	967:973	The LOD of the resulting method	967:997	The LOD of the resulting method was 0.8-1.5 fmol for the individual disaccharides and the LOQ was between 2.5-5 fmol.
31547957	7	10	theme	method	992:997	arg1	0.8-1.5 fmol					1003:1014	0.8-1.5 fmol	1003:1014	0.8-1.5 fmol	1003:1014	The LOD of the resulting method was 0.8-1.5 fmol for the individual disaccharides and the LOQ was between 2.5-5 fmol.
31547957	8	11	theme	Outstanding	1085:1095	arg1	linearity					1097:1105	Outstanding linearity	1085:1105	Outstanding linearity	1085:1105	Outstanding linearity could be observed up to 2 pmol.
31547957	6	12	theme	3-step	848:853	arg1	gradient					855:862	A 3-step gradient	846:862	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration	846:913	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	6	12	theme	3-step	848:853	arg1	ideal					928:932	ideal	928:932	ideal	928:932	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	2	13	theme	methodology	300:310	arg1	improvement					268:278	the constant improvement	255:278	the constant improvement of their analytical methodology	255:310	This necessitates the constant improvement of their analytical methodology.
31547957	4	14	theme	detection	624:632	arg1	limits					634:639	detection limits	624:639	detection limits	624:639	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	5	15	theme	salt	781:784	arg1	method					795:800	a salt gradient method	779:800	a salt gradient method in order to maximize separation efficiency	779:843	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	6	16	from	14 mM	869:873	arg1	gradient					855:862	A 3-step gradient	846:862	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration	846:913	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	6	16	from	14 mM	869:873	arg1	ideal					928:932	ideal	928:932	ideal	928:932	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	3	17	theme	sulfate	500:506	arg1	disaccharides					508:520	heparan sulfate disaccharides	492:520	heparan sulfate disaccharides	492:520	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	2	18	theme	analytical	289:298	arg1	methodology					300:310	their analytical methodology	283:310	their analytical methodology	283:310	This necessitates the constant improvement of their analytical methodology.
31547957	3	19	theme	isocratic	457:465	arg1	separations					477:487	isocratic HILIC-WAX separations	457:487	isocratic HILIC-WAX separations of heparan sulfate disaccharides	457:520	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	3	20	theme	solvent	366:372	arg1	composition					374:384	solvent composition	366:384	solvent composition	366:384	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	4	21	theme	combined	527:534	arg1	use					536:538	The combined use	523:538	The combined use of 75% acetonitrile with ammonium formate	523:580	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	22	theme	heparan	1263:1269	arg1	sulfate					1271:1277	heparan sulfate	1263:1277	heparan sulfate samples from porcine and bovine origin	1263:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	3	23	theme	HILIC-WAX	467:475	arg1	separations					477:487	isocratic HILIC-WAX separations	457:487	isocratic HILIC-WAX separations of heparan sulfate disaccharides	457:520	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	5	24	theme	method	795:800	arg1	use					772:774	the potential use	758:774	the potential use of a salt gradient method in order to maximize separation efficiency	758:843	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	3	25	theme	type	416:419	arg1	effects					355:361	the chromatographic effects	335:361	the chromatographic effects of solvent composition, salt concentration, and salt type	335:419	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	1	26	theme	chromatographic	183:197	arg1	point					222:226	chromatographic and mass spectrometric point	183:226	chromatographic and mass spectrometric point of view	183:234	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	4	27	theme	overall	586:592	arg1	benefits					594:601	overall benefits	586:601	overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated	586:696	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	28	theme	sulfate	1271:1277	arg1	samples					1279:1285	heparan sulfate samples	1263:1285	heparan sulfate samples from porcine and bovine origin	1263:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	3	29	theme	salt	387:390	arg1	concentration					392:404	salt concentration	387:404	salt concentration	387:404	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	9	30	theme	samples	1279:1285	arg1	analysis					1251:1258	the analysis	1247:1258	the analysis of heparan sulfate samples from porcine and bovine origin	1247:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	4	31	contain	had	582:584	arg2	benefits					594:601	overall benefits	586:601	overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated	586:696	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	4	31	contain	had	582:584	arg1	use					536:538	The combined use	523:538	The combined use of 75% acetonitrile with ammonium formate	523:580	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	32	theme	novel	1144:1148	arg1	combination					1150:1160	This novel combination	1139:1160	This novel combination	1139:1160	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	3	33	theme	heparan	492:498	arg1	sulfate					500:506	heparan sulfate	492:506	heparan sulfate disaccharides	492:520	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	0	34	theme	solvent	9:15	arg1	effects					17:23	solvent effects	9:23	solvent effects	9:23	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	1	35	theme	spectrometric	208:220	arg1	point					222:226	chromatographic and mass spectrometric point	183:226	chromatographic and mass spectrometric point of view	183:234	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	5	36	theme	potential	762:770	arg1	use					772:774	the potential use	758:774	the potential use of a salt gradient method in order to maximize separation efficiency	758:843	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	5	37	theme	separation	823:832	arg1	efficiency					834:843	separation efficiency	823:843	separation efficiency	823:843	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	4	38	with	use	536:538	arg1	formate					574:580	ammonium formate	565:580	ammonium formate	565:580	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	39	theme	sufficient	1171:1180	arg1	sensitivity					1182:1192	sufficient sensitivity	1171:1192	sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin	1171:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	4	40	theme	salt	665:668	arg1	concentrations					670:683	all salt concentrations	661:683	all salt concentrations investigated	661:696	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	0	41	theme	chromatographic	43:57	arg1	separation					59:68	the microscale chromatographic separation	28:68	the microscale chromatographic separation of heparan sulfate	28:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	4	42	theme	acetonitrile	547:558	arg1	use					536:538	The combined use	523:538	The combined use of 75% acetonitrile with ammonium formate	523:580	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	3	43	theme	present	320:326	arg1	study					328:332	the present study	316:332	the present study	316:332	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	6	44	theme	formate	893:899	arg1	concentration					901:913	65 mM ammonium formate concentration	878:913	65 mM ammonium formate concentration	878:913	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	0	45	theme	microscale	32:41	arg1	separation					59:68	the microscale chromatographic separation	28:68	the microscale chromatographic separation of heparan sulfate	28:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	7	46	theme	resulting	982:990	arg1	method					992:997	the resulting method	978:997	the resulting method	978:997	The LOD of the resulting method was 0.8-1.5 fmol for the individual disaccharides and the LOQ was between 2.5-5 fmol.
31547957	6	47	theme	ammonium	884:891	arg1	concentration					901:913	65 mM ammonium formate concentration	878:913	65 mM ammonium formate concentration	878:913	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	1	48	theme	heparan	120:126	arg1	sulfate					128:134	heparan sulfate	120:134	heparan sulfate disaccharides	120:148	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	4	49	theme	ammonium	565:572	arg1	formate					574:580	ammonium formate	565:580	ammonium formate	565:580	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	5	50	theme	isocratic	725:733	arg1	measurements					735:746	the isocratic measurements	721:746	the isocratic measurements	721:746	Results obtained with the isocratic measurements suggested the potential use of a salt gradient method in order to maximize separation efficiency.
31547957	6	51	theme	65 mM	878:882	arg1	concentration					901:913	65 mM ammonium formate concentration	878:913	65 mM ammonium formate concentration	878:913	A 3-step gradient from 14 mM to 65 mM ammonium formate concentration proved to be ideal for separation and quantitation.
31547957	1	52	dep	point	222:226	arg1	both					173:176	both	173:176	both	173:176	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	9	53	theme	disaccharide	1198:1209	arg1	analysis					1211:1218	disaccharide analysis	1198:1218	disaccharide analysis	1198:1218	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	1	54	theme	sulfate	128:134	arg1	disaccharides					136:148	heparan sulfate disaccharides	120:148	heparan sulfate disaccharides	120:148	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	1	55	theme	view	231:234	arg1	point					222:226	chromatographic and mass spectrometric point	183:226	chromatographic and mass spectrometric point of view	183:234	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	4	56	theme	75	543:544	arg1	%					545:545	%	545:545	%	545:545	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31547957	9	57	from	porcine	1292:1298	arg1	analysis					1251:1258	the analysis	1247:1258	the analysis of heparan sulfate samples from porcine and bovine origin	1247:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	9	57	from	porcine	1292:1298	arg1	samples					1279:1285	heparan sulfate samples	1263:1285	heparan sulfate samples from porcine and bovine origin	1263:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	8	58	dep	pmol	1133:1136	arg1	up					1125:1126	up	1125:1126	up	1125:1126	Outstanding linearity could be observed up to 2 pmol.
31547957	1	59	theme	disaccharides	136:148	arg1	analysis					108:115	The analysis	104:115	The analysis of heparan sulfate disaccharides	104:148	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	3	60	theme	disaccharides	508:520	arg1	separations					477:487	isocratic HILIC-WAX separations	457:487	isocratic HILIC-WAX separations of heparan sulfate disaccharides	457:520	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	3	61	theme	chromatographic	339:353	arg1	effects					355:361	the chromatographic effects	335:361	the chromatographic effects of solvent composition, salt concentration, and salt type	335:419	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	2	62	theme	constant	259:266	arg1	improvement					268:278	the constant improvement	255:278	the constant improvement of their analytical methodology	255:310	This necessitates the constant improvement of their analytical methodology.
31547957	1	63	theme	mass	203:206	arg1	point					222:226	chromatographic and mass spectrometric point	183:226	chromatographic and mass spectrometric point of view	183:234	The analysis of heparan sulfate disaccharides poses a real challenge both from chromatographic and mass spectrometric point of view.
31547957	3	64	theme	salt	411:414	arg1	type					416:419	salt type	411:419	salt type	411:419	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	0	65	from	effects	17:23	arg1	separation					59:68	the microscale chromatographic separation	28:68	the microscale chromatographic separation of heparan sulfate	28:87	Salt and solvent effects in the microscale chromatographic separation of heparan sulfate disaccharides.
31547957	3	66	theme	concentration	392:404	arg1	effects					355:361	the chromatographic effects	335:361	the chromatographic effects of solvent composition, salt concentration, and salt type	335:419	In the present study, the chromatographic effects of solvent composition, salt concentration, and salt type were systematically investigated in isocratic HILIC-WAX separations of heparan sulfate disaccharides.
31547957	9	67	from	analysis	1251:1258	arg1	porcine					1292:1298	porcine	1292:1298	porcine	1292:1298	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	9	67	from	analysis	1251:1258	arg1	origin					1311:1316	bovine origin	1304:1316	bovine origin	1304:1316	This novel combination provided sufficient sensitivity for disaccharide analysis, which was demonstrated by the analysis of heparan sulfate samples from porcine and bovine origin.
31547957	4	68	theme	peak	646:649	arg1	shape					651:655	peak shape	646:655	peak shape	646:655	The combined use of 75% acetonitrile with ammonium formate had overall benefits regarding intensity, detection limits, and peak shape for all salt concentrations investigated.
31792892	4	0	theme	fucose	738:743	arg1	derivatives					745:755	environmentally questionable fucose derivatives	709:755	environmentally questionable fucose derivatives	709:755	In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied.
31792892	1	1	theme	due	190:192	arg1	oligosaccharides					147:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	1	1	theme	due	190:192	arg1	molecules					180:188	interesting molecules	168:188	interesting molecules due to their bioactive properties	168:222	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	7	2	dep	KM	1212:1213	arg1	the					1208:1210	the	1208:1210	the	1208:1210	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	9	3	theme	trisaccharide	1399:1411	arg1	structure					1374:1382	The structure	1370:1382	The structure of the product trisaccharide	1370:1411	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	9	3	theme	trisaccharide	1399:1411	arg1	1-fucosyllactose					1428:1443	1-fucosyllactose	1428:1443	1-fucosyllactose	1428:1443	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	3	4	theme	study	353:357	arg1	objective					335:343	The objective	331:343	The objective of this study	331:357	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	7	5	theme	hydrolysis	1133:1142	arg1	reaction					1144:1151	the same hydrolysis reaction	1124:1151	the same hydrolysis reaction	1124:1151	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	4	6	theme	questionable	725:736	arg1	derivatives					745:755	environmentally questionable fucose derivatives	709:755	environmentally questionable fucose derivatives	709:755	In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied.
31792892	11	7	theme	non-reducing	1787:1798	arg1	1-fucosyllactose					1800:1815	a novel non-reducing 1-fucosyllactose	1779:1815	a novel non-reducing 1-fucosyllactose	1779:1815	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	0	8	theme	novel	98:102	arg1	1-fucosyllactose					117:132	a novel non-reducing 1-fucosyllactose	96:132	a novel non-reducing 1-fucosyllactose	96:132	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	7	9	theme	same	1128:1131	arg1	reaction					1144:1151	the same hydrolysis reaction	1124:1151	the same hydrolysis reaction	1124:1151	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	5	10	theme	niger	830:834	arg1	α-fucosidase					836:847	a recombinantly produced A. niger α-fucosidase	802:847	a recombinantly produced A. niger α-fucosidase	802:847	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	3	11	theme	hydrolase	392:400	arg1	α-fucosidase					412:423	the glycosyl hydrolase family 29 α-fucosidase	379:423	the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger	379:453	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	3	12	theme	lactose	589:595	arg1	stream					602:607	the lactose side stream	585:607	the lactose side stream produced by dairy industry	585:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	2	13	theme	dairy	313:317	arg1	industries					319:328	dairy industries	313:328	dairy industries	313:328	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	0	14	theme	1-fucosyllactose	117:132	arg1	production					82:91	production	82:91	production of a novel non-reducing 1-fucosyllactose	82:132	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	0	14	theme	1-fucosyllactose	117:132	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	0	14	theme	1-fucosyllactose	117:132	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	9	15	theme	anomeric	1478:1485	arg1	carbon					1487:1492	the anomeric carbon	1474:1492	the anomeric carbon of the β-glucose moiety of lactose	1474:1527	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	9	16	attach	α-linked	1462:1469	arg2	fucose					1452:1457	fucose	1452:1457	fucose	1452:1457	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	9	16	attach	α-linked	1462:1469	arg1	carbon					1487:1492	the anomeric carbon	1474:1492	the anomeric carbon of the β-glucose moiety of lactose	1474:1527	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	7	17	theme	30-min	989:994	arg1	reaction					996:1003	a 30-min reaction	987:1003	a 30-min reaction	987:1003	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	2	18	from	application	246:256	arg1	powders					287:293	milk powders	282:293	milk powders	282:293	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	4	19	theme	free	686:689	arg1	fucose					691:696	free fucose	686:696	free fucose	686:696	In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied.
31792892	0	20	theme	non-reducing	104:115	arg1	1-fucosyllactose					117:132	a novel non-reducing 1-fucosyllactose	96:132	a novel non-reducing 1-fucosyllactose	96:132	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	5	21	theme	produced	818:825	arg1	α-fucosidase					836:847	a recombinantly produced A. niger α-fucosidase	802:847	a recombinantly produced A. niger α-fucosidase	802:847	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	10	22	theme	own	1580:1582	arg1	product					1602:1608	its own transfucosylation product	1576:1608	its own transfucosylation product	1576:1608	Furthermore, the enzyme was able to hydrolyze its own transfucosylation product and 2'-fucosyllactose but only poorly 3-fucosyllactose.
31792892	1	23	theme	bioactive	203:211	arg1	properties					213:222	their bioactive properties	197:222	their bioactive properties	197:222	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	10	24	theme	transfucosylation	1584:1600	arg1	product					1602:1608	its own transfucosylation product	1576:1608	its own transfucosylation product	1576:1608	Furthermore, the enzyme was able to hydrolyze its own transfucosylation product and 2'-fucosyllactose but only poorly 3-fucosyllactose.
31792892	6	25	theme	hydrolytic	913:922	arg1	activity					924:931	hydrolytic activity	913:931	hydrolytic activity	913:931	Using pNP-fucose as substrate, the optimal pH for hydrolytic activity was determined to be 3.8.
31792892	11	26	from	niger	1704:1708	arg1	α-fucosidase					1683:1694	α-fucosidase	1683:1694	α-fucosidase from A. niger	1683:1708	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	3	27	theme	Aspergillus	437:447	arg1	niger					449:453	Aspergillus niger	437:453	Aspergillus niger	437:453	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	7	28	theme	24-h	1085:1088	arg1	reaction					1090:1097	a 24-h reaction	1083:1097	a 24-h reaction	1083:1097	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	8	29	theme	several	1356:1362	arg1	days					1364:1367	several days	1356:1367	several days	1356:1367	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	4	30	theme	fucose	691:696	arg1	use					679:681	the use	675:681	the use of free fucose instead of environmentally questionable fucose derivatives	675:755	In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied.
31792892	3	31	theme	family	402:407	arg1	α-fucosidase					412:423	the glycosyl hydrolase family 29 α-fucosidase	379:423	the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger	379:453	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	11	32	theme	novel	1781:1785	arg1	1-fucosyllactose					1800:1815	a novel non-reducing 1-fucosyllactose	1779:1815	a novel non-reducing 1-fucosyllactose	1779:1815	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	3	33	theme	glycosyl	383:390	arg1	α-fucosidase					412:423	the glycosyl hydrolase family 29 α-fucosidase	379:423	the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger	379:453	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	7	34	theme	optimal	963:969	arg1	temperature					971:981	The optimal temperature	959:981	The optimal temperature for a 30-min reaction	959:1003	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	3	35	theme	side	597:600	arg1	stream					602:607	the lactose side stream	585:607	the lactose side stream produced by dairy industry	585:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	3	36	theme	stream	602:607	arg1	valorization					569:580	the valorization	565:580	the valorization of the lactose side stream produced by dairy industry	565:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	9	37	theme	lactose	1521:1527	arg1	moiety					1511:1516	the β-glucose moiety	1497:1516	the β-glucose moiety of lactose	1497:1527	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	9	38	theme	β-glucose	1501:1509	arg1	moiety					1511:1516	the β-glucose moiety	1497:1516	the β-glucose moiety of lactose	1497:1527	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	0	39	theme	niger	47:51	arg1	α-fucosidase					65:76	Aspergillus niger GH29 family α-fucosidase	35:76	Aspergillus niger GH29 family α-fucosidase	35:76	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	3	40	theme	industrial	533:542	arg1	valorization					569:580	the valorization	565:580	the valorization of the lactose side stream produced by dairy industry	565:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	3	40	theme	industrial	533:542	arg1	applications					544:555	potential industrial applications	523:555	potential industrial applications such as the valorization of the lactose side stream produced by dairy industry	523:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	5	41	dep	studies	793:799	arg1	contrast					773:780	contrast	773:780	contrast	773:780	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	0	42	theme	Aspergillus	35:45	arg1	α-fucosidase					65:76	Aspergillus niger GH29 family α-fucosidase	35:76	Aspergillus niger GH29 family α-fucosidase	35:76	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	5	43	theme	A.	827:828	arg1	α-fucosidase					836:847	a recombinantly produced A. niger α-fucosidase	802:847	a recombinantly produced A. niger α-fucosidase	802:847	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	6	44	theme	optimal	898:904	arg1	3.8					954:956	3.8	954:956	3.8	954:956	Using pNP-fucose as substrate, the optimal pH for hydrolytic activity was determined to be 3.8.
31792892	6	44	theme	optimal	898:904	arg1	pH					906:907	the optimal pH	894:907	the optimal pH for hydrolytic activity	894:931	Using pNP-fucose as substrate, the optimal pH for hydrolytic activity was determined to be 3.8.
31792892	2	45	theme	active	261:266	arg1	ingredient					268:277	active ingredient	261:277	active ingredient	261:277	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	5	46	theme	earlier	785:791	arg1	studies					793:799	earlier studies	785:799	earlier studies	785:799	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	0	47	theme	family	58:63	arg1	α-fucosidase					65:76	Aspergillus niger GH29 family α-fucosidase	35:76	Aspergillus niger GH29 family α-fucosidase	35:76	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	2	48	from	particular	228:237	arg1	attractive					298:307	attractive	298:307	attractive	298:307	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	7	49	theme	*	1231:1231	arg1	KM					1212:1213	KM	1212:1213	KM	1212:1213	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	7	49	theme	*	1231:1231	arg1	h					1232:1232	mg*h	1229:1232	mg*h	1229:1232	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	3	50	theme	dairy	621:625	arg1	industry					627:634	dairy industry	621:634	dairy industry	621:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	7	51	theme	optimal	1059:1065	arg1	temperature					1067:1077	the optimal temperature	1055:1077	the optimal temperature for a 24-h reaction	1055:1097	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	7	51	theme	optimal	1059:1065	arg1	45 °C					1114:1118	45 °C	1114:1118	45 °C For the same hydrolysis reaction	1114:1151	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	3	52	theme	potential	523:531	arg1	valorization					569:580	the valorization	565:580	the valorization of the lactose side stream produced by dairy industry	565:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	3	52	theme	potential	523:531	arg1	applications					544:555	potential industrial applications	523:555	potential industrial applications such as the valorization of the lactose side stream produced by dairy industry	523:634	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	0	53	theme	GH29	53:56	arg1	α-fucosidase					65:76	Aspergillus niger GH29 family α-fucosidase	35:76	Aspergillus niger GH29 family α-fucosidase	35:76	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	3	54	gly	transfucosylate	479:493	arg1	lactose					495:501	lactose	495:501	lactose	495:501	The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry.
31792892	7	55	theme	temperature	1032:1042	arg1	stability					1044:1052	temperature stability	1032:1052	temperature stability	1032:1052	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	1	56	theme	Fucosylated	135:145	arg1	oligosaccharides					147:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	1	56	theme	Fucosylated	135:145	arg1	molecules					180:188	interesting molecules	168:188	interesting molecules due to their bioactive properties	168:222	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	5	57	used	utilized	853:860	arg2	α-fucosidase					836:847	a recombinantly produced A. niger α-fucosidase	802:847	a recombinantly produced A. niger α-fucosidase	802:847	In contrast to earlier studies, a recombinantly produced A. niger α-fucosidase was utilized.
31792892	7	58	theme	kinetic	1158:1164	arg1	values					1166:1171	the kinetic values	1154:1171	the kinetic values	1154:1171	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	7	58	theme	kinetic	1158:1164	arg1	0.385 mM					1195:1202	0.385 mM	1195:1202	0.385 mM	1195:1202	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	8	59	theme	high	1290:1293	arg1	concentrations					1305:1318	high substrate concentrations	1290:1318	high substrate concentrations	1290:1318	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	2	60	from	attractive	298:307	arg1	particular					228:237	particular	228:237	particular	228:237	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	8	61	gly	Transfucosylation	1249:1265	arg1	lactose					1270:1276	lactose	1270:1276	lactose	1270:1276	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	8	61	gly	Transfucosylation	1249:1265	arg2	concentrations					1305:1318	high substrate concentrations	1290:1318	high substrate concentrations	1290:1318	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	9	62	theme	moiety	1511:1516	arg1	carbon					1487:1492	the anomeric carbon	1474:1492	the anomeric carbon of the β-glucose moiety of lactose	1474:1527	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	8	63	theme	reaction	1325:1332	arg1	time					1334:1337	reaction time	1325:1337	reaction time	1325:1337	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	11	64	gly	transfucosylate	1714:1728	arg1	conclusion					1671:1680	a conclusion	1669:1680	a conclusion	1669:1680	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	11	64	gly	transfucosylate	1714:1728	arg1	lactose					1730:1736	lactose	1730:1736	lactose	1730:1736	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	7	65	theme	mg	1229:1230	arg1	KM					1212:1213	KM	1212:1213	KM	1212:1213	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	7	65	theme	mg	1229:1230	arg1	h					1232:1232	mg*h	1229:1232	mg*h	1229:1232	The optimal temperature for a 30-min reaction was 60 °C, and considering temperature stability, the optimal temperature for a 24-h reaction was defined as 45 °C For the same hydrolysis reaction, the kinetic values were calculated to be 0.385 mM for the KM and 2.8 mmol/(mg*h) for the Vmax.
31792892	2	66	theme	milk	282:285	arg1	powders					287:293	milk powders	282:293	milk powders	282:293	In particular, their application as active ingredient in milk powders is attractive for dairy industries.
31792892	1	67	theme	interesting	168:178	arg1	oligosaccharides					147:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides	135:162	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	1	67	theme	interesting	168:178	arg1	molecules					180:188	interesting molecules	168:188	interesting molecules due to their bioactive properties	168:222	Fucosylated oligosaccharides are interesting molecules due to their bioactive properties.
31792892	11	68	theme	free	1744:1747	arg1	fucose					1749:1754	free fucose	1744:1754	free fucose	1744:1754	As a conclusion, α-fucosidase from A. niger can transfucosylate lactose using free fucose as substrate producing a novel non-reducing 1-fucosyllactose.
31792892	0	69	theme	α-fucosidase	65:76	arg1	production					82:91	production	82:91	production of a novel non-reducing 1-fucosyllactose	82:132	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	0	69	theme	α-fucosidase	65:76	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	0	69	theme	α-fucosidase	65:76	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose.
31792892	9	70	theme	product	1391:1397	arg1	trisaccharide					1399:1411	the product trisaccharide	1387:1411	the product trisaccharide	1387:1411	The structure of the product trisaccharide was defined as 1-fucosyllactose, where fucose is α-linked to the anomeric carbon of the β-glucose moiety of lactose.
31792892	8	71	theme	substrate	1295:1303	arg1	concentrations					1305:1318	high substrate concentrations	1290:1318	high substrate concentrations	1290:1318	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	8	72	theme	lactose	1270:1276	arg1	Transfucosylation					1249:1265	Transfucosylation	1249:1265	Transfucosylation of lactose	1249:1276	Transfucosylation of lactose occurred at high substrate concentrations when reaction time was elongated to several days.
31792892	4	73	theme	derivatives	745:755	arg1	use					679:681	the use	675:681	the use of free fucose instead of environmentally questionable fucose derivatives	675:755	In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied.
30936009	7	0	theme	MRP-1	1153:1157	arg1	treatment					1159:1167	MRP-1 treatment	1153:1167	MRP-1 treatment	1153:1167	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	7	1	theme	expression	1071:1080	arg1	level					1082:1086	the mRNA expression level	1062:1086	the mRNA expression level of iNOS induced by LPS	1062:1109	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	2	2	theme	hot	347:349	arg1	method					368:373	hot water extraction method	347:373	hot water extraction method followed by ethanol precipitation	347:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	6	3	theme	TNF-α	953:957	arg1	production					959:968	TNF-α production	953:968	TNF-α production	953:968	The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment.
30936009	6	4	theme	treatment	1041:1049	arg1	concentrations					1017:1030	different concentrations	1007:1030	different concentrations of MRP-1 treatment	1007:1049	The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment.
30936009	7	5	dep	decreased	1115:1123	arg1	p < 0.05					1140:1147	p < 0.05	1140:1147	p < 0.05	1140:1147	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	7	6	from	effect	1191:1196	arg1	expression					1216:1225	the COX-2 mRNA expression	1201:1225	the COX-2 mRNA expression	1201:1225	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	8	7	theme	Moringa	1279:1285	arg1	polysaccharide					1301:1314	Moringa oleifera root polysaccharide	1279:1314	Moringa oleifera root polysaccharide	1279:1314	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	5	8	theme	MRP-1	892:896	arg1	activity					880:887	the anti-inflammatory activity	858:887	the anti-inflammatory activity of MRP-1	858:896	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	7	9	theme	mRNA	1066:1069	arg1	level					1082:1086	the mRNA expression level	1062:1086	the mRNA expression level of iNOS induced by LPS	1062:1109	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	7	10	theme	obvious	1183:1189	arg1	effect					1191:1196	no obvious effect	1180:1196	no obvious effect on the COX-2 mRNA expression	1180:1225	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	4	11	theme	typical	686:692	arg1	features					694:701	the typical features	682:701	the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly	682:768	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	7	12	theme	mRNA	1211:1214	arg1	expression					1216:1225	the COX-2 mRNA expression	1201:1225	the COX-2 mRNA expression	1201:1225	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	6	13	theme	MRP-1	1035:1039	arg1	treatment					1041:1049	MRP-1 treatment	1035:1049	MRP-1 treatment	1035:1049	The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment.
30936009	8	14	theme	root	1296:1299	arg1	polysaccharide					1301:1314	Moringa oleifera root polysaccharide	1279:1314	Moringa oleifera root polysaccharide	1279:1314	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	2	15	theme	ethanol	387:393	arg1	precipitation					395:407	ethanol precipitation	387:407	ethanol precipitation	387:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	7	16	theme	iNOS	1091:1094	arg1	level					1082:1086	the mRNA expression level	1062:1086	the mRNA expression level of iNOS induced by LPS	1062:1109	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	1	17	theme	Moringa	95:101	arg1	plant					138:142	a mutli-purpose herbal plant	115:142	a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine	115:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	17	theme	Moringa	95:101	arg1	oleifera					103:110	Moringa oleifera	95:110	Moringa oleifera	95:110	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	18	theme	natural	187:193	arg1	source					195:200	a natural source	185:200	a natural source of nutrients and folk medicine	185:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	18	theme	natural	187:193	arg1	attention					172:180	enormous attention	163:180	enormous attention	163:180	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	4	19	theme	carbohydrates	706:718	arg1	features					694:701	the typical features	682:701	the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly	682:768	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	7	20	theme	COX-2	1205:1209	arg1	expression					1216:1225	the COX-2 mRNA expression	1201:1225	the COX-2 mRNA expression	1201:1225	Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression.
30936009	5	21	theme	macrophage	819:828	arg1	cells					830:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	2	22	theme	Flow	447:450	arg1	column					452:457	DEAE-Sepharose Fast Flow column	427:457	DEAE-Sepharose Fast Flow column	427:457	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	5	23	theme	LPS-induced	798:808	arg1	cells					830:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	5	24	theme	RAW264.7	810:817	arg1	cells					830:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	6	25	theme	different	1007:1015	arg1	concentrations					1017:1030	different concentrations	1007:1030	different concentrations of MRP-1 treatment	1007:1049	The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment.
30936009	0	26	theme	oleifera	28:35	arg1	MRP-1					58:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	2	27	attach	isolated	305:312	arg2	polysaccharide					265:278	a novel polysaccharide	257:278	a novel polysaccharide	257:278	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	2	27	attach	isolated	305:312	arg1	roots					336:340	Moringa oleifera roots	319:340	Moringa oleifera roots with hot water extraction method followed by ethanol precipitation	319:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	8	28	theme	possible	1255:1262	arg1	application					1264:1274	new possible application	1251:1274	new possible application of Moringa oleifera root polysaccharide related to anti-inflammation	1251:1343	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	1	29	theme	nutrients	205:213	arg1	source					195:200	a natural source	185:200	a natural source of nutrients and folk medicine	185:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	29	theme	nutrients	205:213	arg1	attention					172:180	enormous attention	163:180	enormous attention	163:180	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	0	30	theme	Moringa	20:26	arg1	MRP-1					58:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	1	31	theme	mutli-purpose	117:129	arg1	plant					138:142	a mutli-purpose herbal plant	115:142	a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine	115:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	31	theme	mutli-purpose	117:129	arg1	oleifera					103:110	Moringa oleifera	95:110	Moringa oleifera	95:110	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	4	32	theme	NMR	657:659	arg1	analysis					661:668	NMR analysis	657:668	NMR analysis	657:668	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	4	32	theme	NMR	657:659	arg1	spectra					638:644	The Roman spectra	628:644	The Roman spectra	628:644	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	0	33	theme	polysaccharide	43:56	arg1	MRP-1					58:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	5	34	theme	anti-inflammatory	862:878	arg1	activity					880:887	the anti-inflammatory activity	858:887	the anti-inflammatory activity of MRP-1	858:896	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	1	35	theme	folk	219:222	arg1	medicine					224:231	folk medicine	219:231	folk medicine	219:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	0	36	theme	roots	37:41	arg1	MRP-1					58:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Moringa oleifera roots polysaccharide MRP-1	20:62	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	2	37	with	roots	336:340	arg1	method					368:373	hot water extraction method	347:373	hot water extraction method followed by ethanol precipitation	347:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	2	38	theme	Fast	442:445	arg1	column					452:457	DEAE-Sepharose Fast Flow column	427:457	DEAE-Sepharose Fast Flow column	427:457	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	1	39	theme	medicine	224:231	arg1	source					195:200	a natural source	185:200	a natural source of nutrients and folk medicine	185:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	39	theme	medicine	224:231	arg1	attention					172:180	enormous attention	163:180	enormous attention	163:180	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	8	40	theme	related	1316:1322	arg1	application					1264:1274	new possible application	1251:1274	new possible application of Moringa oleifera root polysaccharide related to anti-inflammation	1251:1343	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	3	41	theme	1.5:2.0:3.1:6.0:5.3:1.1	603:625	arg1	ratio					594:598	the molar ratio	584:598	the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1	584:625	Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1.
30936009	5	42	used	used	841:844	arg2	cells					830:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells	794:834	The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1.
30936009	8	43	theme	polysaccharide	1301:1314	arg1	application					1264:1274	new possible application	1251:1274	new possible application of Moringa oleifera root polysaccharide related to anti-inflammation	1251:1343	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	1	44	theme	herbal	131:136	arg1	plant					138:142	a mutli-purpose herbal plant	115:142	a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine	115:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	44	theme	herbal	131:136	arg1	oleifera					103:110	Moringa oleifera	95:110	Moringa oleifera	95:110	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	0	45	theme	MRP-1	58:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.	0:93	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	2	46	theme	oleifera	327:334	arg1	roots					336:340	Moringa oleifera roots	319:340	Moringa oleifera roots with hot water extraction method followed by ethanol precipitation	319:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	3	47	theme	molar	588:592	arg1	ratio					594:598	the molar ratio	584:598	the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1	584:625	Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1.
30936009	2	48	theme	novel	259:263	arg1	polysaccharide					265:278	a novel polysaccharide	257:278	a novel polysaccharide	257:278	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	0	49	with	Characterization	0:15	arg1	effect					87:92	anti-inflammatory effect	69:92	anti-inflammatory effect	69:92	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	2	50	theme	Moringa	319:325	arg1	roots					336:340	Moringa oleifera roots	319:340	Moringa oleifera roots with hot water extraction method followed by ethanol precipitation	319:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	8	51	theme	oleifera	1287:1294	arg1	polysaccharide					1301:1314	Moringa oleifera root polysaccharide	1279:1314	Moringa oleifera root polysaccharide	1279:1314	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	8	52	theme	new	1251:1253	arg1	application					1264:1274	new possible application	1251:1274	new possible application of Moringa oleifera root polysaccharide related to anti-inflammation	1251:1343	This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.
30936009	1	53	theme	enormous	163:170	arg1	source					195:200	a natural source	185:200	a natural source of nutrients and folk medicine	185:231	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	1	53	theme	enormous	163:170	arg1	attention					172:180	enormous attention	163:180	enormous attention	163:180	Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine.
30936009	3	54	theme	Monosaccharide	460:473	arg1	analysis					487:494	Monosaccharide composition analysis	460:494	Monosaccharide composition analysis based on GC-MS	460:509	Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1.
30936009	3	55	theme	composition	475:485	arg1	analysis					487:494	Monosaccharide composition analysis	460:494	Monosaccharide composition analysis based on GC-MS	460:509	Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1.
30936009	2	56	theme	extraction	357:366	arg1	method					368:373	hot water extraction method	347:373	hot water extraction method followed by ethanol precipitation	347:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	4	57	theme	Roman	632:636	arg1	analysis					661:668	NMR analysis	657:668	NMR analysis	657:668	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	4	57	theme	Roman	632:636	arg1	spectra					638:644	The Roman spectra	628:644	The Roman spectra	628:644	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	4	57	theme	Roman	632:636	arg1	FT-IR					647:651	FT-IR	647:651	FT-IR	647:651	The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1.
30936009	0	58	theme	anti-inflammatory	69:85	arg1	effect					87:92	anti-inflammatory effect	69:92	anti-inflammatory effect	69:92	Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.
30936009	2	59	theme	DEAE-Sepharose	427:440	arg1	column					452:457	DEAE-Sepharose Fast Flow column	427:457	DEAE-Sepharose Fast Flow column	427:457	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
30936009	2	60	theme	water	351:355	arg1	method					368:373	hot water extraction method	347:373	hot water extraction method followed by ethanol precipitation	347:407	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.
31054308	4	0	theme	light	543:547	arg1	scattering					549:558	dynamic light scattering	535:558	dynamic light scattering	535:558	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	5	1	theme	2:1	676:678	arg1	ratio					680:684	a ca. 2:1 ratio	670:684	a ca. 2:1 ratio	670:684	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	8	2	theme	starch	1088:1093	arg1	mats					1101:1104	micro-sized pure starch fiber mats	1071:1104	micro-sized pure starch fiber mats	1071:1104	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	5	3	theme	complex	642:648	arg1	component					608:616	a minor component	600:616	a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun	600:713	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	3	theme	complex	642:648	arg1	Pullulan					575:582	Pullulan	575:582	Pullulan	575:582	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	4	4	theme	dispersions	487:497	arg1	potential					457:465	zeta potential	452:465	zeta potential	452:465	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	4	4	theme	dispersions	487:497	arg1	properties					437:446	Flow properties	432:446	Flow properties	432:446	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	7	5	theme	differential	934:945	arg1	calorimetry					956:966	differential scanning calorimetry	934:966	differential scanning calorimetry	934:966	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	8	6	theme	composite	1025:1033	arg1	strength					999:1006	Tensile strength	991:1006	Tensile strength of the nanofiber composite	991:1033	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	9	7	theme	organic	1186:1192	arg1	chemicals					1194:1202	organic chemicals	1186:1202	organic chemicals	1186:1202	This method provides future industry with lower cost by eliminating the use of organic chemicals.
31054308	5	8	theme	starch	651:656	arg1	pullulan					658:665	starch:pullulan	651:665	starch:pullulan	651:665	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	2	9	from	starch	252:257	arg1	water					262:266	water	262:266	water	262:266	High-temperature (≈162 °C) was used to destructure high-amylose starch in water.
31054308	0	10	from	dispersions	56:66	arg1	mats					22:25	Electrospun nanofiber mats	0:25	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	4	11	theme	Flow	432:435	arg1	properties					437:446	Flow properties	432:446	Flow properties	432:446	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	6	12	theme	starch	734:739	arg1	association					741:751	starch association	734:751	starch association	734:751	Pullulan hindered starch association and modified the dispersion properties, promoting molecular entanglement without gelation.
31054308	4	13	theme	dynamic	535:541	arg1	scattering					549:558	dynamic light scattering	535:558	dynamic light scattering	535:558	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	7	14	from	presence	848:855	arg1	fiber					911:915	the fiber	907:915	the fiber	907:915	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	0	15	theme	Electrospun	0:10	arg1	mats					22:25	Electrospun nanofiber mats	0:25	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	9	16	theme	lower	1149:1153	arg1	cost					1155:1158	lower cost	1149:1158	lower cost	1149:1158	This method provides future industry with lower cost by eliminating the use of organic chemicals.
31054308	0	17	theme	nanofiber	12:20	arg1	mats					22:25	Electrospun nanofiber mats	0:25	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	5	18	theme	mixture	695:701	arg1	electrospun					703:713	the mixture electrospun	691:713	the mixture electrospun	691:713	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	3	19	theme	electrospinning	410:424	arg1	dope					426:429	the electrospinning dope	406:429	the electrospinning dope	406:429	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	3	20	theme	Sodium	269:274	arg1	palmitate					276:284	Sodium palmitate	269:284	Sodium palmitate	269:284	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	8	21	theme	pure	1083:1086	arg1	mats					1101:1104	micro-sized pure starch fiber mats	1071:1104	micro-sized pure starch fiber mats	1071:1104	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	7	22	theme	complexes	894:902	arg1	presence					848:855	The presence	844:855	The presence of sodium palmitate-starch inclusion complexes in the fiber	844:915	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	7	23	theme	sodium	860:865	arg1	complexes					894:902	sodium palmitate-starch inclusion complexes	860:902	sodium palmitate-starch inclusion complexes	860:902	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	5	24	theme	starch-palmitate	625:640	arg1	complex					642:648	the starch-palmitate complex	621:648	the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio)	621:685	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	7	25	attach	presence	848:855	arg1	fiber					911:915	the fiber	907:915	the fiber	907:915	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	7	25	attach	presence	848:855	arg2	complexes					894:902	sodium palmitate-starch inclusion complexes	860:902	sodium palmitate-starch inclusion complexes	860:902	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	3	26	from	temperature	361:371	arg1	water					347:351	water	347:351	water at room temperature	347:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	3	26	from	temperature	361:371	arg1	stability					311:319	the stability	307:319	the stability of high-amylose starch in water at room temperature	307:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	1	27	theme	green	125:129	arg1	method					131:136	A green method	123:136	A green method to fabricate starch-based nanofibers	123:173	A green method to fabricate starch-based nanofibers is provided.
31054308	7	28	theme	palmitate-starch	867:882	arg1	complexes					894:902	sodium palmitate-starch inclusion complexes	860:902	sodium palmitate-starch inclusion complexes	860:902	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	0	29	theme	starch-pullulan	40:54	arg1	dispersions					56:66	aqueous starch-pullulan dispersions	32:66	aqueous starch-pullulan dispersions	32:66	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	0	30	theme	Optimizing	69:78	arg1	properties					91:100	Optimizing dispersion properties	69:100	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	4	31	theme	starch-palmitate	470:485	arg1	dispersions					487:497	starch-palmitate dispersions	470:497	starch-palmitate dispersions	470:497	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	7	32	theme	inclusion	884:892	arg1	complexes					894:902	sodium palmitate-starch inclusion complexes	860:902	sodium palmitate-starch inclusion complexes	860:902	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	0	33	theme	aqueous	32:38	arg1	dispersions					56:66	aqueous starch-pullulan dispersions	32:66	aqueous starch-pullulan dispersions	32:66	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	8	34	theme	micro-sized	1071:1081	arg1	mats					1101:1104	micro-sized pure starch fiber mats	1071:1104	micro-sized pure starch fiber mats	1071:1104	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	3	35	theme	dope	426:429	arg1	conductivity					390:401	the conductivity	386:401	the conductivity of the electrospinning dope	386:429	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	3	36	theme	high-amylose	324:335	arg1	starch					337:342	high-amylose starch	324:342	high-amylose starch	324:342	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	2	37	used	used	219:222	arg2	High-temperature					188:203	High-temperature	188:203	High-temperature (≈162 °C)	188:213	High-temperature (≈162 °C) was used to destructure high-amylose starch in water.
31054308	2	37	used	used	219:222	arg2	≈162 °C					206:212	≈162 °C	206:212	≈162 °C	206:212	High-temperature (≈162 °C) was used to destructure high-amylose starch in water.
31054308	8	38	theme	Tensile	991:997	arg1	strength					999:1006	Tensile strength	991:1006	Tensile strength of the nanofiber composite	991:1033	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	3	39	theme	starch	337:342	arg1	stability					311:319	the stability	307:319	the stability of high-amylose starch in water at room temperature	307:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	4	40	theme	zeta	452:455	arg1	potential					457:465	zeta potential	452:465	zeta potential	452:465	Flow properties and zeta potential of starch-palmitate dispersions were characterized by rheometer and dynamic light scattering, respectively.
31054308	2	41	theme	high-amylose	239:250	arg1	starch					252:257	high-amylose starch	239:257	high-amylose starch in water	239:266	High-temperature (≈162 °C) was used to destructure high-amylose starch in water.
31054308	9	42	theme	chemicals	1194:1202	arg1	use					1179:1181	the use	1175:1181	the use of organic chemicals	1175:1202	This method provides future industry with lower cost by eliminating the use of organic chemicals.
31054308	5	43	theme	ca.	672:674	arg1	ratio					680:684	a ca. 2:1 ratio	670:684	a ca. 2:1 ratio	670:684	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	44	from	ratio	680:684	arg1	pullulan					658:665	starch:pullulan	651:665	starch:pullulan	651:665	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	45	theme	electrospun	703:713	arg1	component					608:616	a minor component	600:616	a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun	600:713	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	45	theme	electrospun	703:713	arg1	Pullulan					575:582	Pullulan	575:582	Pullulan	575:582	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	46	theme	minor	602:606	arg1	component					608:616	a minor component	600:616	a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun	600:713	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	5	46	theme	minor	602:606	arg1	Pullulan					575:582	Pullulan	575:582	Pullulan	575:582	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	3	47	from	stability	311:319	arg1	water					347:351	water	347:351	water at room temperature	347:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	3	47	from	stability	311:319	arg1	temperature					361:371	room temperature	356:371	room temperature	356:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	9	48	with	industry	1135:1142	arg1	cost					1155:1158	lower cost	1149:1158	lower cost	1149:1158	This method provides future industry with lower cost by eliminating the use of organic chemicals.
31054308	0	49	dep	mats	22:25	arg1	properties					91:100	Optimizing dispersion properties	69:100	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	6	50	theme	dispersion	770:779	arg1	properties					781:790	the dispersion properties	766:790	the dispersion properties	766:790	Pullulan hindered starch association and modified the dispersion properties, promoting molecular entanglement without gelation.
31054308	1	51	theme	starch-based	151:162	arg1	nanofibers					164:173	starch-based nanofibers	151:173	starch-based nanofibers	151:173	A green method to fabricate starch-based nanofibers is provided.
31054308	5	52	dep	complex	642:648	arg1	pullulan					658:665	starch:pullulan	651:665	starch:pullulan	651:665	Pullulan was mixed in as a minor component of the starch-palmitate complex (starch:pullulan at a ca. 2:1 ratio) and the mixture electrospun.
31054308	0	53	theme	dispersion	80:89	arg1	properties					91:100	Optimizing dispersion properties	69:100	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.	0:121	Electrospun nanofiber mats from aqueous starch-pullulan dispersions: Optimizing dispersion properties for electrospinning.
31054308	9	54	theme	future	1128:1133	arg1	industry					1135:1142	future industry	1128:1142	future industry with lower cost	1128:1158	This method provides future industry with lower cost by eliminating the use of organic chemicals.
31054308	8	55	theme	nanofiber	1015:1023	arg1	composite					1025:1033	the nanofiber composite	1011:1033	the nanofiber composite	1011:1033	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	7	56	theme	X-ray	972:976	arg1	diffraction					978:988	X-ray diffraction	972:988	X-ray diffraction	972:988	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31054308	3	57	theme	room	356:359	arg1	temperature					361:371	room temperature	356:371	room temperature	356:371	Sodium palmitate was added to enhance the stability of high-amylose starch in water at room temperature and increase the conductivity of the electrospinning dope.
31054308	8	58	theme	fiber	1095:1099	arg1	mats					1101:1104	micro-sized pure starch fiber mats	1071:1104	micro-sized pure starch fiber mats	1071:1104	Tensile strength of the nanofiber composite was found to be weaker than that of micro-sized pure starch fiber mats.
31054308	7	59	theme	scanning	947:954	arg1	calorimetry					956:966	differential scanning calorimetry	934:966	differential scanning calorimetry	934:966	The presence of sodium palmitate-starch inclusion complexes in the fiber was confirmed by differential scanning calorimetry and X-ray diffraction.
31761224	7	0	theme	good	1589:1592	arg1	activity					1606:1613	good antioxidant activity	1589:1613	good antioxidant activity	1589:1613	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	4	1	theme	20μ	1114:1116	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	6	2	theme	antioxidant	1373:1383	arg1	investigation					1385:1397	In-vitro antioxidant investigation	1364:1397	In-vitro antioxidant investigation through DPPH assay	1364:1416	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	1	3	theme	humrur	452:457	arg1	skins					408:412	the skins	404:412	the skins of Marine big eye snapper Priacanthus humrur	404:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	1	4	theme	processing	257:266	arg1	wastes					268:273	fish processing wastes	252:273	fish processing wastes	252:273	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	5	theme	Collagen/gelatin/Chitosan	1121:1145	arg1	composite					1147:1155	Collagen/gelatin/Chitosan composite	1121:1155	Collagen/gelatin/Chitosan composite	1121:1155	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	8	6	theme	diffusion	1739:1747	arg1	assay					1749:1753	disc diffusion assay	1734:1753	disc diffusion assay	1734:1753	The scaffolds were also evaluated for anti microbial properties through disc diffusion assay.
31761224	3	7	theme	mechanical	758:767	arg1	composition					795:805	their mechanical property.Gelatin/chitosan composition	752:805	their mechanical property.Gelatin/chitosan composition	752:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	4	8	theme	2-5μ	1202:1205	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	6	9	from	3	1444:1444	arg1	concentration					1457:1469	1 mg/ml concentration	1449:1469	1 mg/ml concentration	1449:1469	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	9	10	theme	inhibition-	1783:1793	arg1	14 mm					1795:1799	maximum inhibition- 14 mm	1775:1799	maximum inhibition- 14 mm	1775:1799	The results showed maximum inhibition- 14 mm, 12 mm and 14 mm for 200 μg of the sample for collagen/chitosan, gelatin/chitosan and collagen/gelatin/chitosan scaffolds respectively towards E.Coli and 20 mm, 20 mm and 24 mm towards S.aureus.
31761224	4	11	theme	collagen/chitosan	1210:1226	arg1	composites					1228:1237	collagen/chitosan composites	1210:1237	collagen/chitosan composites	1210:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	4	12	from	composites	1187:1196	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	2	13	theme	solutions	558:566	arg1	mixing					527:532	mixing	527:532	mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers	527:632	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	0	14	theme	antimicrobial	138:150	arg1	applications					169:180	antimicrobial and anti oxidant applications	138:180	antimicrobial and anti oxidant applications	138:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	2	15	theme	subsequent	598:607	arg1	crosslinking					609:620	subsequent crosslinking	598:620	subsequent crosslinking	598:620	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	9	16	dep	gelatin/chitosan	1866:1881	arg1	scaffolds					1913:1921	scaffolds	1913:1921	scaffolds	1913:1921	The results showed maximum inhibition- 14 mm, 12 mm and 14 mm for 200 μg of the sample for collagen/chitosan, gelatin/chitosan and collagen/gelatin/chitosan scaffolds respectively towards E.Coli and 20 mm, 20 mm and 24 mm towards S.aureus.
31761224	0	17	theme	anti	156:159	arg1	applications					169:180	antimicrobial and anti oxidant applications	138:180	antimicrobial and anti oxidant applications	138:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	1	18	theme	big	424:426	arg1	humrur					452:457	Marine big eye snapper Priacanthus humrur	417:457	Marine big eye snapper Priacanthus humrur	417:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	11	19	theme	study	2168:2172	arg1	findings					2152:2159	The findings	2148:2159	The findings of the study	2148:2172	The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
31761224	1	20	theme	snapper	432:438	arg1	humrur					452:457	Marine big eye snapper Priacanthus humrur	417:457	Marine big eye snapper Priacanthus humrur	417:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	21	theme	Scanning	1045:1052	arg1	Microscopy					1063:1072	Scanning Electron Microscopy	1045:1072	Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1045:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	0	22	theme	composite	69:77	arg1	scaffolds					79:87	gelatin composite scaffolds	61:87	gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	61:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	2	23	theme	chitosan	511:518	arg1	spinning					499:506	mechanical spinning	488:506	mechanical spinning of chitosan	488:518	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	3	24	dep	capacity	733:740	arg1	assess					745:750	assess	745:750	to assess their mechanical property.Gelatin/chitosan composition	742:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	25	theme	higher	876:881	arg1	density					883:889	higher density	876:889	higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3)	876:989	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	6	26	theme	higher	1481:1486	arg1	potential					1500:1508	higher antioxidant potential	1481:1508	higher antioxidant potential	1481:1508	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	4	27	from	20μ	1114:1116	arg1	composite					1147:1155	Collagen/gelatin/Chitosan composite	1121:1155	Collagen/gelatin/Chitosan composite	1121:1155	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	4	27	from	20μ	1114:1116	arg1	composites					1228:1237	collagen/chitosan composites	1210:1237	collagen/chitosan composites	1210:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	4	28	theme	scaffolds	1018:1026	arg1	Morphology					991:1000	Morphology	991:1000	Morphology of the prepared scaffolds	991:1026	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	3	29	theme	collagen	861:868	arg1	incorporation					844:856	incorporation	844:856	incorporation of collagen	844:868	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	29	theme	collagen	861:868	arg1	porosity					823:830	very high porosity	813:830	very high porosity(81.02%)	813:838	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	29	theme	collagen	861:868	arg1	%					837:837	81.02%	832:837	81.02%	832:837	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	0	30	theme	eye	98:100	arg1	skin					129:132	big eye snapper Priacanthus hamrur skin	94:132	big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	94:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	0	31	theme	Priacanthus	110:120	arg1	skin					129:132	big eye snapper Priacanthus hamrur skin	94:132	big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	94:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	4	32	dep	10μ	1163:1165	arg1	to					1160:1161	to	1160:1161	to	1160:1161	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	1	33	theme	novel	315:319	arg1	scaffold					328:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	9	34	theme	sample	1836:1841	arg1	200 μg					1822:1827	200 μg	1822:1827	200 μg of the sample	1822:1841	The results showed maximum inhibition- 14 mm, 12 mm and 14 mm for 200 μg of the sample for collagen/chitosan, gelatin/chitosan and collagen/gelatin/chitosan scaffolds respectively towards E.Coli and 20 mm, 20 mm and 24 mm towards S.aureus.
31761224	5	35	theme	intense	1261:1267	arg1	peaks					1269:1273	intense peaks	1261:1273	intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups	1261:1361	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	11	36	theme	tissue	2247:2252	arg1	engineering					2254:2264	tissue engineering	2247:2264	tissue engineering	2247:2264	The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
31761224	1	37	theme	collagen/gelatin/chitosan	289:313	arg1	scaffold					328:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	7	38	theme	inhibition	1642:1651	arg1	percentage					1628:1637	highest percentage	1620:1637	highest percentage of inhibition (29.5%)	1620:1659	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	0	39	theme	chitosan	36:43	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	0	39	theme	chitosan	36:43	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	1	40	theme	porous	321:326	arg1	scaffold					328:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	41	theme	pore	1095:1098	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	3	42	theme	rheological	668:678	arg1	properties					680:689	rheological properties	668:689	rheological properties	668:689	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	7	43	dep	identified	1552:1561	arg1	had					1585:1587	had	1585:1587	identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%)	1552:1659	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	3	44	theme	density	712:718	arg1	capacity					733:740	porosity, apparent density and swelling capacity	693:740	porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition	693:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	5	45	theme	different	1313:1321	arg1	scaffolds					1323:1331	the three different scaffolds	1303:1331	the three different scaffolds that are denoted as CH groups	1303:1361	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	5	45	theme	different	1313:1321	arg1	groups					1356:1361	CH groups	1353:1361	CH groups	1353:1361	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	7	46	theme	antioxidant	1594:1604	arg1	activity					1606:1613	good antioxidant activity	1589:1613	good antioxidant activity	1589:1613	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	4	47	theme	10μ	1163:1165	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	0	48	from	skin	129:132	arg1	scaffolds					79:87	gelatin composite scaffolds	61:87	gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	61:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	6	49	theme	antioxidant	1488:1498	arg1	potential					1500:1508	higher antioxidant potential	1481:1508	higher antioxidant potential	1481:1508	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	1	50	theme	fish	252:255	arg1	wastes					268:273	fish processing wastes	252:273	fish processing wastes	252:273	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	2	51	theme	polymers	625:632	arg1	drying					587:592	freeze drying	580:592	freeze drying	580:592	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	2	51	theme	polymers	625:632	arg1	crosslinking					609:620	subsequent crosslinking	598:620	subsequent crosslinking	598:620	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	3	52	theme	swelling	724:731	arg1	capacity					733:740	porosity, apparent density and swelling capacity	693:740	porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition	693:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	6	53	theme	In-vitro	1364:1371	arg1	investigation					1385:1397	In-vitro antioxidant investigation	1364:1397	In-vitro antioxidant investigation through DPPH assay	1364:1416	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	7	54	theme	ABTS	1530:1533	arg1	assay					1546:1550	ABTS scavenging assay	1530:1550	ABTS scavenging assay	1530:1550	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	8	55	theme	disc	1734:1737	arg1	assay					1749:1753	disc diffusion assay	1734:1753	disc diffusion assay	1734:1753	The scaffolds were also evaluated for anti microbial properties through disc diffusion assay.
31761224	3	56	theme	property.Gelatin/chitosan	769:793	arg1	composition					795:805	their mechanical property.Gelatin/chitosan composition	752:805	their mechanical property.Gelatin/chitosan composition	752:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	4	57	from	composites	1228:1237	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	7	58	theme	scavenging	1535:1544	arg1	assay					1546:1550	ABTS scavenging assay	1530:1550	ABTS scavenging assay	1530:1550	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	9	59	theme	maximum	1775:1781	arg1	14 mm					1795:1799	maximum inhibition- 14 mm	1775:1799	maximum inhibition- 14 mm	1775:1799	The results showed maximum inhibition- 14 mm, 12 mm and 14 mm for 200 μg of the sample for collagen/chitosan, gelatin/chitosan and collagen/gelatin/chitosan scaffolds respectively towards E.Coli and 20 mm, 20 mm and 24 mm towards S.aureus.
31761224	11	60	contain	have	2222:2225	arg1	bioscaffolds					2193:2204	the bioscaffolds	2189:2204	the bioscaffolds	2189:2204	The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
31761224	11	60	contain	have	2222:2225	arg2	application					2232:2242	wide application	2227:2242	wide application	2227:2242	The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
31761224	11	61	theme	wide	2227:2230	arg1	application					2232:2242	wide application	2227:2242	wide application	2227:2242	The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
31761224	10	62	theme	inhibition	2008:2017	arg1	zone					2000:2003	The zone	1996:2003	The zone of inhibition against E. coli and S. aureus for the three scaffolds	1996:2071	The zone of inhibition against E. coli and S. aureus for the three scaffolds was comparatively lower and that could be due to the presence of chitosan.
31761224	10	62	theme	inhibition	2008:2017	arg1	lower					2091:2095	lower	2091:2095	lower	2091:2095	The zone of inhibition against E. coli and S. aureus for the three scaffolds was comparatively lower and that could be due to the presence of chitosan.
31761224	4	63	theme	gelatin/chitosan	1170:1185	arg1	composites					1187:1196	gelatin/chitosan composites	1170:1196	gelatin/chitosan composites	1170:1196	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	3	64	theme	high	818:821	arg1	porosity					823:830	very high porosity	813:830	very high porosity(81.02%)	813:838	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	64	theme	high	818:821	arg1	%					837:837	81.02%	832:837	81.02%	832:837	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	1	65	from	wastes	268:273	arg1	engineering					235:245	tissue engineering	228:245	tissue engineering from fish processing wastes	228:273	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	7	66	from	composite1	1563:1572	arg1	1 mg/ml					1577:1583	1 mg/ml	1577:1583	1 mg/ml	1577:1583	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	2	67	theme	gelatin	550:556	arg1	solutions					558:566	collagen and gelatin solutions	537:566	collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers	537:632	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	0	68	theme	oxidant	161:167	arg1	applications					169:180	antimicrobial and anti oxidant applications	138:180	antimicrobial and anti oxidant applications	138:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	1	69	theme	Marine	417:422	arg1	humrur					452:457	Marine big eye snapper Priacanthus humrur	417:457	Marine big eye snapper Priacanthus humrur	417:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	70	from	10μ	1163:1165	arg1	composites					1187:1196	gelatin/chitosan composites	1170:1196	gelatin/chitosan composites	1170:1196	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	6	71	theme	DPPH	1407:1410	arg1	assay					1412:1416	DPPH assay	1407:1416	DPPH assay	1407:1416	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	1	72	theme	eye	428:430	arg1	humrur					452:457	Marine big eye snapper Priacanthus humrur	417:457	Marine big eye snapper Priacanthus humrur	417:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	2	73	theme	freeze	580:585	arg1	drying					587:592	freeze drying	580:592	freeze drying	580:592	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	1	74	theme	Priacanthus	440:450	arg1	humrur					452:457	Marine big eye snapper Priacanthus humrur	417:457	Marine big eye snapper Priacanthus humrur	417:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	75	from	composite	1147:1155	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	3	76	theme	Collagen/gelatin/chitosan	894:918	arg1	scaffolds					920:928	Collagen/gelatin/chitosan scaffolds	894:928	Collagen/gelatin/chitosan scaffolds (0.0522g/cm3)	894:942	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	76	theme	Collagen/gelatin/chitosan	894:918	arg1	0.0522g/cm3					931:941	0.0522g/cm3	931:941	0.0522g/cm3	931:941	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	4	77	theme	prepared	1009:1016	arg1	scaffolds					1018:1026	the prepared scaffolds	1005:1026	the prepared scaffolds	1005:1026	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	0	78	theme	big	94:96	arg1	skin					129:132	big eye snapper Priacanthus hamrur skin	94:132	big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	94:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	3	79	dep	shown	807:811	arg1	shown					870:874	shown	870:874	shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3)	807:989	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	80	theme	collagen/chitosan	948:964	arg1	0.0468 g/cm3					977:988	0.0468 g/cm3	977:988	0.0468 g/cm3	977:988	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	80	theme	collagen/chitosan	948:964	arg1	scaffolds					966:974	collagen/chitosan scaffolds	948:974	collagen/chitosan scaffolds (0.0468 g/cm3)	948:989	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	4	81	theme	Electron	1054:1061	arg1	Microscopy					1063:1072	Scanning Electron Microscopy	1045:1072	Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1045:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	5	82	theme	CH	1353:1354	arg1	scaffolds					1323:1331	the three different scaffolds	1303:1331	the three different scaffolds that are denoted as CH groups	1303:1361	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	5	82	theme	CH	1353:1354	arg1	groups					1356:1361	CH groups	1353:1361	CH groups	1353:1361	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	10	83	theme	chitosan	2138:2145	arg1	presence					2126:2133	the presence	2122:2133	the presence of chitosan	2122:2145	The zone of inhibition against E. coli and S. aureus for the three scaffolds was comparatively lower and that could be due to the presence of chitosan.
31761224	0	84	theme	snapper	102:108	arg1	skin					129:132	big eye snapper Priacanthus hamrur skin	94:132	big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	94:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	1	85	theme	collagen	368:375	arg1	blends					358:363	blends	358:363	blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur	358:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	6	86	dep	exhibited	1471:1479	arg1	%					1513:1513	70%	1511:1513	70%	1511:1513	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	2	87	theme	collagen	537:544	arg1	solutions					558:566	collagen and gelatin solutions	537:566	collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers	537:632	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	0	88	theme	hamrur	122:127	arg1	skin					129:132	big eye snapper Priacanthus hamrur skin	94:132	big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	94:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	1	89	theme	gelatin	381:387	arg1	blends					358:363	blends	358:363	blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur	358:457	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	4	90	dep	20μ	1114:1116	arg1	to					1111:1112	to	1111:1112	to	1111:1112	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	6	91	theme	1 mg/ml	1449:1455	arg1	concentration					1457:1469	1 mg/ml concentration	1449:1469	1 mg/ml concentration	1449:1469	In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%).
31761224	1	92	theme	hierachial	278:287	arg1	scaffold					328:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	a hierachial collagen/gelatin/chitosan novel porous scaffold	276:335	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	5	93	theme	1120	1283:1286	arg1	-11267 cm-1					1288:1298	1120 -11267 cm-1	1283:1298	1120 -11267 cm-1	1283:1298	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	4	94	from	size	1100:1103	arg1	composites					1187:1196	gelatin/chitosan composites	1170:1196	gelatin/chitosan composites	1170:1196	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	4	94	from	size	1100:1103	arg1	composite					1147:1155	Collagen/gelatin/Chitosan composite	1121:1155	Collagen/gelatin/Chitosan composite	1121:1155	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	4	94	from	size	1100:1103	arg1	composites					1228:1237	collagen/chitosan composites	1210:1237	collagen/chitosan composites	1210:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	2	95	theme	mechanical	488:497	arg1	spinning					499:506	mechanical spinning	488:506	mechanical spinning of chitosan	488:518	Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers.
31761224	3	96	from	density	883:889	arg1	scaffolds					920:928	Collagen/gelatin/chitosan scaffolds	894:928	Collagen/gelatin/chitosan scaffolds (0.0522g/cm3)	894:942	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	96	from	density	883:889	arg1	0.0468 g/cm3					977:988	0.0468 g/cm3	977:988	0.0468 g/cm3	977:988	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	96	from	density	883:889	arg1	scaffolds					966:974	collagen/chitosan scaffolds	948:974	collagen/chitosan scaffolds (0.0468 g/cm3)	948:989	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	3	96	from	density	883:889	arg1	0.0522g/cm3					931:941	0.0522g/cm3	931:941	0.0522g/cm3	931:941	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	0	97	theme	gelatin	61:67	arg1	scaffolds					79:87	gelatin composite scaffolds	61:87	gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications	61:180	Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications.
31761224	5	98	theme	FTIR	1240:1243	arg1	analysis					1245:1252	FTIR analysis	1240:1252	FTIR analysis	1240:1252	FTIR analysis showed intense peaks ranging 1120 -11267 cm-1 in the three different scaffolds that are denoted as CH groups.
31761224	4	99	theme	reduced	1087:1093	arg1	size					1100:1103	reduced pore size	1087:1103	reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites	1087:1237	Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20μ in Collagen/gelatin/Chitosan composite, 5 to 10μ in gelatin/chitosan composites and 2-5μ in collagen/chitosan composites.
31761224	3	100	theme	apparent	703:710	arg1	density					712:718	apparent density	703:718	apparent density	703:718	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	7	101	theme	highest	1620:1626	arg1	percentage					1628:1637	highest percentage	1620:1637	highest percentage of inhibition (29.5%)	1620:1659	In contrast, ABTS scavenging assay identified composite1 in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%).
31761224	3	102	theme	porosity	693:700	arg1	capacity					733:740	porosity, apparent density and swelling capacity	693:740	porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition	693:805	The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm3) and collagen/chitosan scaffolds (0.0468 g/cm3).
31761224	1	103	theme	tissue	228:233	arg1	engineering					235:245	tissue engineering	228:245	tissue engineering from fish processing wastes	228:273	In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur.
31761224	8	104	theme	microbial	1705:1713	arg1	properties					1715:1724	anti microbial properties	1700:1724	anti microbial properties	1700:1724	The scaffolds were also evaluated for anti microbial properties through disc diffusion assay.
31761224	8	105	theme	anti	1700:1703	arg1	properties					1715:1724	anti microbial properties	1700:1724	anti microbial properties	1700:1724	The scaffolds were also evaluated for anti microbial properties through disc diffusion assay.
31786298	0	0	theme	alginate/pectin	86:100	arg1	ratios					76:81	different blend ratios	60:81	different blend ratios of alginate/pectin	60:100	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	5	1	theme	BSA	946:948	arg1	tests					914:918	swelling tests	905:918	swelling tests	905:918	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	5	1	theme	BSA	946:948	arg1	release					935:941	the higher release	924:941	the higher release of BSA in drug-release testing	924:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	6	2	theme	cell	1048:1051	arg1	cytotoxicity					1053:1064	cell cytotoxicity	1048:1064	cell cytotoxicity	1048:1064	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	0	3	link	cross-linked	26:37	arg1	composite					39:47	ionic cross-linked composite	20:47	ionic cross-linked composite	20:47	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	3	4	theme	alginate-pectin	505:519	arg1	foams					531:535	alginate-pectin composite foams	505:535	alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking	505:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	8	5	from	use	1355:1357	arg1	applications					1377:1388	wound-dressing applications	1362:1388	wound-dressing applications	1362:1388	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	7	6	theme	alginate-pectin	1166:1180	arg1	foams					1182:1186	alginate-pectin foams	1166:1186	alginate-pectin foams	1166:1186	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	8	7	from	candidates	1340:1349	arg1	addition					1284:1291	addition	1284:1291	addition	1284:1291	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	3	8	theme	blending	552:559	arg1	ratios					561:566	different blending ratios	542:566	different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking	542:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	5	9	from	levels	835:840	arg1	foams					859:863	the composite foams	845:863	the composite foams	845:863	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	1	10	theme	synergistic	279:289	arg1	interaction					291:301	their synergistic interaction	273:301	their synergistic interaction	273:301	Alginate and pectin have been widely employed together in various industrial and biomedical applications due to their synergistic interaction.
31786298	7	11	theme	water	1237:1241	arg1	absorption					1243:1252	water absorption	1237:1252	water absorption	1237:1252	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	3	12	with	foams	531:535	arg1	ratios					561:566	different blending ratios	542:566	different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking	542:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	4	13	theme	alginate-pectin	702:716	arg1	foam					722:725	rehydrated alginate-pectin 9:1 foam	691:725	rehydrated alginate-pectin 9:1 foam	691:725	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	4	14	theme	rehydrated	691:700	arg1	foam					722:725	rehydrated alginate-pectin 9:1 foam	691:725	rehydrated alginate-pectin 9:1 foam	691:725	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	2	15	from	materials	361:369	arg1	gels					381:384	gels	381:384	gels	381:384	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	15	from	materials	361:369	arg1	particles					391:399	particles	391:399	particles	391:399	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	15	from	materials	361:369	arg1	films					374:378	films	374:378	films	374:378	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	8	16	theme	wound-dressing	1362:1375	arg1	applications					1377:1388	wound-dressing applications	1362:1388	wound-dressing applications	1362:1388	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	5	17	theme	pectin	828:833	arg1	levels					835:840	higher pectin levels	821:840	higher pectin levels in the composite foams	821:863	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	0	18	theme	synergistic	109:119	arg1	effects					121:127	the synergistic effects	105:127	the synergistic effects for wound dressing application	105:158	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	3	19	theme	different	542:550	arg1	ratios					561:566	different blending ratios	542:566	different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking	542:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	6	20	theme	foams	1032:1036	arg1	none					1020:1023	none	1020:1023	none of the foams	1020:1036	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	4	21	theme	foam	722:725	arg1	higher					731:736	higher	731:736	higher	731:736	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	4	21	theme	foam	722:725	arg1	values					681:686	the G' values	674:686	the G' values of rehydrated alginate-pectin 9:1 foam	674:725	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	7	22	theme	pectin	1148:1153	arg1	content					1155:1161	the pectin content	1144:1161	the pectin content in alginate-pectin foams	1144:1186	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	4	23	theme	9:1	718:720	arg1	foam					722:725	rehydrated alginate-pectin 9:1 foam	691:725	rehydrated alginate-pectin 9:1 foam	691:725	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	7	24	theme	drug-release	1259:1270	arg1	ability					1272:1278	drug-release ability	1259:1278	drug-release ability	1259:1278	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	0	25	theme	cross-linked	26:37	arg1	composite					39:47	ionic cross-linked composite	20:47	ionic cross-linked composite	20:47	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	0	26	theme	wound	133:137	arg1	application					148:158	wound dressing application	133:158	wound dressing application	133:158	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	8	27	theme	alginate-pectin	1294:1308	arg1	foams					1320:1324	alginate-pectin composite foams	1294:1324	alginate-pectin composite foams	1294:1324	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	8	27	theme	alginate-pectin	1294:1308	arg1	candidates					1340:1349	promising candidates	1330:1349	promising candidates for use in wound-dressing applications	1330:1388	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	0	28	theme	ionic	20:24	arg1	composite					39:47	ionic cross-linked composite	20:47	ionic cross-linked composite	20:47	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	5	29	theme	swelling	905:912	arg1	tests					914:918	swelling tests	905:918	swelling tests	905:918	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	7	30	from	content	1155:1161	arg1	foams					1182:1186	alginate-pectin foams	1166:1186	alginate-pectin foams	1166:1186	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	7	31	theme	mechanical	1214:1223	arg1	properties					1225:1234	their mechanical properties	1208:1234	their mechanical properties	1208:1234	These results suggest that controlling the pectin content in alginate-pectin foams is key to adjusting their mechanical properties, water absorption, and drug-release ability.
31786298	5	32	theme	composite	849:857	arg1	foams					859:863	the composite foams	845:863	the composite foams	845:863	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	6	33	theme	cytotoxicity	998:1009	arg1	testing					1011:1017	indirect and direct cytotoxicity testing	978:1017	indirect and direct cytotoxicity testing	978:1017	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	4	34	theme	rheological	786:796	arg1	behavior					798:805	the rheological behavior	782:805	the rheological behavior	782:805	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	1	35	theme	various	219:225	arg1	applications					253:264	various industrial and biomedical applications	219:264	various industrial and biomedical applications due to their synergistic interaction	219:301	Alginate and pectin have been widely employed together in various industrial and biomedical applications due to their synergistic interaction.
31786298	0	36	theme	composite	39:47	arg1	Characterization					0:15	Characterization	0:15	Characterization of ionic cross-linked composite	0:47	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	0	37	theme	dressing	139:146	arg1	application					148:158	wound dressing application	133:158	wound dressing application	133:158	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	1	38	theme	industrial	227:236	arg1	applications					253:264	various industrial and biomedical applications	219:264	various industrial and biomedical applications due to their synergistic interaction	219:301	Alginate and pectin have been widely employed together in various industrial and biomedical applications due to their synergistic interaction.
31786298	0	39	theme	different	60:68	arg1	ratios					76:81	different blend ratios	60:81	different blend ratios of alginate/pectin	60:100	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	6	40	theme	direct	991:996	arg1	testing					1011:1017	indirect and direct cytotoxicity testing	978:1017	indirect and direct cytotoxicity testing	978:1017	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	5	41	theme	higher	928:933	arg1	release					935:941	the higher release	924:941	the higher release of BSA in drug-release testing	924:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	5	42	theme	more	872:875	arg1	water					877:881	more water	872:881	more water being absorbed during swelling tests and the higher release of BSA in drug-release testing	872:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	3	43	theme	present	476:482	arg1	study					484:488	the present study	472:488	the present study	472:488	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	6	44	theme	keratinocyte	1085:1096	arg1	cells					1098:1102	fibroblast and keratinocyte cells	1070:1102	cells	1098:1102	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	5	45	from	tests	914:918	arg1	testing					966:972	drug-release testing	953:972	drug-release testing	953:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	4	46	theme	rehydrated	762:771	arg1	foams					773:777	the other rehydrated foams	752:777	the other rehydrated foams in the rheological behavior	752:805	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	3	47	theme	ion	602:604	arg1	cross-linking					606:618	calcium ion cross-linking	594:618	calcium ion cross-linking	594:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	6	48	theme	indirect	978:985	arg1	testing					1011:1017	indirect and direct cytotoxicity testing	978:1017	indirect and direct cytotoxicity testing	978:1017	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	2	49	theme	composite	351:359	arg1	materials					361:369	composite materials	351:369	composite materials in films, gels, and particles	351:399	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	49	theme	composite	351:359	arg1	alginate					313:320	alginate	313:320	alginate	313:320	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	49	theme	composite	351:359	arg1	pectin					326:331	pectin	326:331	pectin	326:331	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	6	50	theme	fibroblast	1070:1079	arg1	cells					1098:1102	fibroblast and keratinocyte cells	1070:1102	cells	1098:1102	In indirect and direct cytotoxicity testing, none of the foams exhibited cell cytotoxicity for fibroblast and keratinocyte cells.
31786298	8	51	theme	promising	1330:1338	arg1	foams					1320:1324	alginate-pectin composite foams	1294:1324	alginate-pectin composite foams	1294:1324	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	8	51	theme	promising	1330:1338	arg1	candidates					1340:1349	promising candidates	1330:1349	promising candidates for use in wound-dressing applications	1330:1388	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	4	52	theme	other	756:760	arg1	foams					773:777	the other rehydrated foams	752:777	the other rehydrated foams in the rheological behavior	752:805	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	3	53	dep	ratios	561:566	arg1	5:5					583:585	5:5	583:585	5:5	583:585	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	3	53	dep	ratios	561:566	arg1	9:1					569:571	9:1	569:571	9:1	569:571	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	3	53	dep	ratios	561:566	arg1	7:3					574:576	7:3	574:576	7:3	574:576	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	8	54	theme	composite	1310:1318	arg1	foams					1320:1324	alginate-pectin composite foams	1294:1324	alginate-pectin composite foams	1294:1324	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	8	54	theme	composite	1310:1318	arg1	candidates					1340:1349	promising candidates	1330:1349	promising candidates for use in wound-dressing applications	1330:1388	In addition, alginate-pectin composite foams are promising candidates for use in wound-dressing applications.
31786298	1	55	theme	biomedical	242:251	arg1	applications					253:264	various industrial and biomedical applications	219:264	various industrial and biomedical applications due to their synergistic interaction	219:301	Alginate and pectin have been widely employed together in various industrial and biomedical applications due to their synergistic interaction.
31786298	5	56	theme	higher	821:826	arg1	levels					835:840	higher pectin levels	821:840	higher pectin levels in the composite foams	821:863	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	5	57	theme	drug-release	953:964	arg1	testing					966:972	drug-release testing	953:972	drug-release testing	953:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	4	58	from	foams	773:777	arg1	behavior					798:805	the rheological behavior	782:805	the rheological behavior	782:805	It was found that the G' values of rehydrated alginate-pectin 9:1 foam was higher than those of the other rehydrated foams in the rheological behavior.
31786298	3	59	theme	composite	521:529	arg1	foams					531:535	alginate-pectin composite foams	505:535	alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking	505:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	2	60	used	used	343:346	arg2	materials					361:369	composite materials	351:369	composite materials in films, gels, and particles	351:399	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	60	used	used	343:346	arg2	pectin					326:331	pectin	326:331	pectin	326:331	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	2	60	used	used	343:346	arg2	alginate					313:320	alginate	313:320	alginate	313:320	Although alginate and pectin have been used as composite materials in films, gels, and particles, research characterizing their properties in foams is scarce.
31786298	0	61	theme	blend	70:74	arg1	ratios					76:81	different blend ratios	60:81	different blend ratios of alginate/pectin	60:100	Characterization of ionic cross-linked composite foams with different blend ratios of alginate/pectin on the synergistic effects for wound dressing application.
31786298	3	62	theme	calcium	594:600	arg1	cross-linking					606:618	calcium ion cross-linking	594:618	calcium ion cross-linking	594:618	Thus, in the present study, we fabricated alginate-pectin composite foams with different blending ratios (9:1, 7:3, and 5:5) using calcium ion cross-linking and characterized their properties.
31786298	5	63	from	release	935:941	arg1	testing					966:972	drug-release testing	953:972	drug-release testing	953:972	In addition, higher pectin levels in the composite foams led to more water being absorbed during swelling tests and the higher release of BSA in drug-release testing.
31786298	1	64	theme	due	266:268	arg1	applications					253:264	various industrial and biomedical applications	219:264	various industrial and biomedical applications due to their synergistic interaction	219:301	Alginate and pectin have been widely employed together in various industrial and biomedical applications due to their synergistic interaction.
30529351	6	0	theme	derivatives	934:944	arg1	DS					921:922	the DS	917:922	the DS of starch derivatives	917:944	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	1	theme	DS	921:922	arg1	increase					905:912	the increase	901:912	the increase of the DS of starch derivatives	901:944	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	1	2	theme	starch	269:274	arg1	time					247:250	the reaction time	234:250	the reaction time of hydroxypropyl starch	234:274	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	0	3	theme	different	85:93	arg1	degree					95:100	different degree	85:100	different degree of substitution under microwave assistance	85:143	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	6	4	theme	starch	927:932	arg1	derivatives					934:944	starch derivatives	927:944	starch derivatives	927:944	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	8	5	theme	starch	1420:1425	arg1	morphology					1401:1410	the surface morphology	1389:1410	the surface morphology of corn starch	1389:1425	Scanning electron microscopy showed that the surface morphology of corn starch was affected to some extent.
30529351	2	6	theme	starch	386:391	arg1	conditions					328:337	microwave assisted conditions	309:337	microwave assisted conditions	309:337	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	2	6	theme	starch	386:391	arg1	properties					359:368	physicochemical properties	343:368	physicochemical properties	343:368	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	0	7	theme	substitution	105:116	arg1	degree					95:100	different degree	85:100	different degree of substitution under microwave assistance	85:143	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	7	8	dep	transform	1195:1203	arg1	infrared					1205:1212	infrared	1205:1212	transform infrared spectroscopy	1195:1225	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	8	9	theme	Scanning	1348:1355	arg1	microscopy					1366:1375	Scanning electron microscopy	1348:1375	Scanning electron microscopy	1348:1375	Scanning electron microscopy showed that the surface morphology of corn starch was affected to some extent.
30529351	2	10	theme	microwave	309:317	arg1	conditions					328:337	microwave assisted conditions	309:337	microwave assisted conditions	309:337	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	6	11	dep	%	1037:1037	arg1	to					1029:1030	to	1029:1030	to	1029:1030	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	12	theme	hydrophobicity	1089:1102	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	12	theme	hydrophobicity	1089:1102	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	12	theme	hydrophobicity	1089:1102	arg1	transparency					951:962	the transparency	947:962	the transparency (4% to 8.81%)	947:976	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	12	theme	hydrophobicity	1089:1102	arg1	ratio					990:994	the volume ratio	979:994	the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g)	979:1125	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	12	theme	hydrophobicity	1089:1102	arg1	%					975:975	4% to 8.81%	965:975	4% to 8.81%	965:975	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	4	13	theme	optimum	541:547	arg1	parameters					549:558	The optimum parameters	537:558	The optimum parameters	537:558	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	5	14	theme	optimal	699:705	arg1	degree					707:712	The optimal degree	695:712	The optimal degree of substitution (DS) of hydroxypropyl starch	695:757	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	1	15	theme	product	288:294	arg1	quality					296:302	product quality	288:302	product quality	288:302	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	0	16	theme	microwave	124:132	arg1	assistance					134:143	microwave assistance	124:143	microwave assistance	124:143	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	2	17	theme	corn	381:384	arg1	starch					386:391	natural corn starch	373:391	natural corn starch	373:391	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	6	18	theme	condensing	1003:1012	arg1	volume					1014:1019	the condensing volume	999:1019	the condensing volume (21.11% to 33.11%)	999:1038	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	18	theme	condensing	1003:1012	arg1	%					1037:1037	21.11% to 33.11%	1022:1037	21.11% to 33.11%	1022:1037	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	3	19	theme	reaction	461:468	arg1	conditions					470:479	the reaction conditions	457:479	the reaction conditions of three-level-three-factorial center composite design	457:534	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	4	20	theme	radiation	628:636	arg1	6.08 min					647:654	6.08 min	647:654	6.08 min	647:654	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	4	20	theme	radiation	628:636	arg1	time					638:641	the radiation time	624:641	the radiation time	624:641	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	6	21	theme	volume	983:988	arg1	ratio					990:994	the volume ratio	979:994	the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g)	979:1125	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	1	22	theme	microwave	188:196	arg1	method					207:212	the microwave assisted method	184:212	the microwave assisted method	184:212	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	6	23	theme	%	1163:1163	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	23	theme	%	1163:1163	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	2	24	theme	physicochemical	343:357	arg1	properties					359:368	physicochemical properties	343:368	physicochemical properties	343:368	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	6	25	dep	ratio	990:994	arg1	0.67 g/g					1105:1112	0.67 g/g	1105:1112	the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g)	979:1125	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	1	26	theme	assisted	198:205	arg1	method					207:212	the microwave assisted method	184:212	the microwave assisted method	184:212	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	9	27	theme	crystalline	1492:1502	arg1	type					1504:1507	the crystalline type	1488:1507	the crystalline type	1488:1507	The modification did not change the crystalline type of starch, which was still A type.
30529351	9	27	theme	crystalline	1492:1502	arg1	type					1538:1541	A type	1536:1541	A type	1536:1541	The modification did not change the crystalline type of starch, which was still A type.
30529351	8	28	theme	corn	1415:1418	arg1	starch					1420:1425	corn starch	1415:1425	corn starch	1415:1425	Scanning electron microscopy showed that the surface morphology of corn starch was affected to some extent.
30529351	7	29	theme	group	1341:1345	arg1	introduction					1311:1322	the successful introduction	1296:1322	the successful introduction of hydroxypropyl group	1296:1345	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	5	30	theme	starch	752:757	arg1	substitution					717:728	substitution	717:728	substitution (DS) of hydroxypropyl starch	717:757	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	5	30	theme	starch	752:757	arg1	DS					731:732	DS	731:732	DS	731:732	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	3	31	theme	Response	407:414	arg1	methodology					424:434	Response surface methodology	407:434	Response surface methodology	407:434	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	4	32	theme	oxide	610:614	arg1	amount					590:595	the additive amount	577:595	the additive amount of propylene oxide	577:614	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	4	32	theme	oxide	610:614	arg1	oxide					610:614	propylene oxide	600:614	propylene oxide	600:614	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	4	32	theme	oxide	610:614	arg1	%					621:621	8%	620:621	8%	620:621	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	6	33	dep	hydrophilic	1045:1055	arg1	0.73 g/g					1058:1065	0.73 g/g	1058:1065	0.73 g/g to 2.46 g/g	1058:1077	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	3	34	theme	design	529:534	arg1	conditions					470:479	the reaction conditions	457:479	the reaction conditions of three-level-three-factorial center composite design	457:534	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	3	35	theme	surface	416:422	arg1	methodology					424:434	Response surface methodology	407:434	Response surface methodology	407:434	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	2	36	theme	assisted	319:326	arg1	conditions					328:337	microwave assisted conditions	309:337	microwave assisted conditions	309:337	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	7	37	from	1149 cm-1	1276:1284	arg1	enhancement					1243:1253	the enhancement	1239:1253	the enhancement of peak intensity at 1149 cm-1	1239:1284	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	6	38	theme	%	966:966	arg1	transparency					951:962	the transparency	947:962	the transparency (4% to 8.81%)	947:976	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	38	theme	%	966:966	arg1	%					975:975	4% to 8.81%	965:975	4% to 8.81%	965:975	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	7	39	dep	Fourier	1187:1193	arg1	transform					1195:1203	transform	1195:1203	transform infrared spectroscopy	1195:1225	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	3	40	theme	center	512:517	arg1	design					529:534	three-level-three-factorial center composite design	484:534	three-level-three-factorial center composite design	484:534	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	0	41	theme	physicochemical	25:39	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	5	42	theme	hydroxypropyl	738:750	arg1	starch					752:757	hydroxypropyl starch	738:757	hydroxypropyl starch	738:757	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	8	43	theme	surface	1393:1399	arg1	morphology					1401:1410	the surface morphology	1389:1410	the surface morphology of corn starch	1389:1425	Scanning electron microscopy showed that the surface morphology of corn starch was affected to some extent.
30529351	7	44	theme	successful	1300:1309	arg1	introduction					1311:1322	the successful introduction	1296:1322	the successful introduction of hydroxypropyl group	1296:1345	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	3	45	theme	composite	519:527	arg1	design					529:534	three-level-three-factorial center composite design	484:534	three-level-three-factorial center composite design	484:534	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	0	46	theme	hydroxypropylated	55:71	arg1	starch					73:78	hydroxypropylated starch	55:78	hydroxypropylated starch	55:78	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	4	47	theme	additive	581:588	arg1	amount					590:595	the additive amount	577:595	the additive amount of propylene oxide	577:614	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	4	47	theme	additive	581:588	arg1	oxide					610:614	propylene oxide	600:614	propylene oxide	600:614	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	4	47	theme	additive	581:588	arg1	%					621:621	8%	620:621	8%	620:621	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	5	48	theme	optimal	781:787	arg1	conditions					789:798	the optimal conditions	777:798	the optimal conditions predicted by RSM, which was 0.0729 mg/g	777:838	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	6	49	theme	evolution	1142:1150	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	49	theme	evolution	1142:1150	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	0	50	with	preparation	9:19	arg1	degree					95:100	different degree	85:100	different degree of substitution under microwave assistance	85:143	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	2	51	dep	conditions	328:337	arg1	The					305:307	The	305:307	The	305:307	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	7	52	theme	hydroxypropyl	1327:1339	arg1	group					1341:1345	hydroxypropyl group	1327:1345	hydroxypropyl group	1327:1345	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	4	53	theme	propylene	600:608	arg1	oxide					610:614	propylene oxide	600:614	propylene oxide	600:614	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	3	54	used	used	440:443	arg2	methodology					424:434	Response surface methodology	407:434	Response surface methodology	407:434	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	6	55	theme	properties	873:882	arg1	analysis					845:852	The analysis	841:852	The analysis of physicochemical properties	841:882	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	56	theme	water	1136:1140	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	56	theme	water	1136:1140	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	2	57	theme	natural	373:379	arg1	starch					386:391	natural corn starch	373:391	natural corn starch	373:391	The microwave assisted conditions and physicochemical properties of natural corn starch were studied.
30529351	8	58	theme	electron	1357:1364	arg1	microscopy					1366:1375	Scanning electron microscopy	1348:1375	Scanning electron microscopy	1348:1375	Scanning electron microscopy showed that the surface morphology of corn starch was affected to some extent.
30529351	5	59	theme	substitution	717:728	arg1	degree					707:712	The optimal degree	695:712	The optimal degree of substitution (DS) of hydroxypropyl starch	695:757	The optimal degree of substitution (DS) of hydroxypropyl starch was determined by the optimal conditions predicted by RSM, which was 0.0729 mg/g.
30529351	6	60	theme	physicochemical	857:871	arg1	properties					873:882	physicochemical properties	857:882	physicochemical properties	857:882	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	7	61	theme	intensity	1263:1271	arg1	enhancement					1243:1253	the enhancement	1239:1253	the enhancement of peak intensity at 1149 cm-1	1239:1284	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	9	62	theme	starch	1512:1517	arg1	type					1504:1507	the crystalline type	1488:1507	the crystalline type	1488:1507	The modification did not change the crystalline type of starch, which was still A type.
30529351	9	62	theme	starch	1512:1517	arg1	type					1538:1541	A type	1536:1541	A type	1536:1541	The modification did not change the crystalline type of starch, which was still A type.
30529351	1	63	theme	reaction	238:245	arg1	time					247:250	the reaction time	234:250	the reaction time of hydroxypropyl starch	234:274	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	6	64	dep	%	975:975	arg1	to					968:969	to	968:969	to	968:969	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	65	theme	%	1027:1027	arg1	volume					1014:1019	the condensing volume	999:1019	the condensing volume (21.11% to 33.11%)	999:1038	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	65	theme	%	1027:1027	arg1	%					1037:1037	21.11% to 33.11%	1022:1037	21.11% to 33.11%	1022:1037	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	66	theme	hydrophilic	1045:1055	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	66	theme	hydrophilic	1045:1055	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	66	theme	hydrophilic	1045:1055	arg1	transparency					951:962	the transparency	947:962	the transparency (4% to 8.81%)	947:976	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	66	theme	hydrophilic	1045:1055	arg1	ratio					990:994	the volume ratio	979:994	the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g)	979:1125	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	66	theme	hydrophilic	1045:1055	arg1	%					975:975	4% to 8.81%	965:975	4% to 8.81%	965:975	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	67	theme	volume	1014:1019	arg1	%					1173:1173	46.91% to 36.16%	1158:1173	46.91% to 36.16%	1158:1173	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	67	theme	volume	1014:1019	arg1	rate					1152:1155	the water evolution rate	1132:1155	the water evolution rate (46.91% to 36.16%)	1132:1174	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	67	theme	volume	1014:1019	arg1	transparency					951:962	the transparency	947:962	the transparency (4% to 8.81%)	947:976	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	67	theme	volume	1014:1019	arg1	ratio					990:994	the volume ratio	979:994	the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g)	979:1125	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	6	67	theme	volume	1014:1019	arg1	%					975:975	4% to 8.81%	965:975	4% to 8.81%	965:975	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	4	68	theme	microwave	665:673	arg1	power					675:679	the microwave power	661:679	the microwave power	661:679	The optimum parameters were as follows: the additive amount of propylene oxide was 8%, the radiation time was 6.08 min, and the microwave power was 303.43 W.
30529351	3	69	theme	three-level-three-factorial	484:510	arg1	design					529:534	three-level-three-factorial center composite design	484:534	three-level-three-factorial center composite design	484:534	Response surface methodology was used to optimize the reaction conditions of three-level-three-factorial center composite design.
30529351	6	70	dep	%	1173:1173	arg1	to					1165:1166	to	1165:1166	to	1165:1166	The analysis of physicochemical properties showed that with the increase of the DS of starch derivatives, the transparency (4% to 8.81%), the volume ratio of the condensing volume (21.11% to 33.11%), the hydrophilic (0.73 g/g to 2.46 g/g), and the hydrophobicity (0.67 g/g to 1.01 g/g), and the water evolution rate (46.91% to 36.16%) decreased.
30529351	0	71	theme	starch	73:78	arg1	preparation					9:19	preparation	9:19	preparation	9:19	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	0	71	theme	starch	73:78	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	7	72	theme	peak	1258:1261	arg1	intensity					1263:1271	peak intensity	1258:1271	peak intensity	1258:1271	Fourier transform infrared spectroscopy showed that the enhancement of peak intensity at 1149 cm-1 indicated the successful introduction of hydroxypropyl group.
30529351	0	73	with	properties	41:50	arg1	degree					95:100	different degree	85:100	different degree of substitution under microwave assistance	85:143	Study on preparation and physicochemical properties of hydroxypropylated starch with different degree of substitution under microwave assistance.
30529351	1	74	theme	traditional	164:174	arg1	method					176:181	the traditional method	160:181	the traditional method	160:181	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30529351	1	75	theme	hydroxypropyl	255:267	arg1	starch					269:274	hydroxypropyl starch	255:274	hydroxypropyl starch	255:274	Compared with the traditional method, the microwave assisted method can greatly shorten the reaction time of hydroxypropyl starch and improve product quality.
30935127	5	0	theme	composite	734:742	arg1	material					744:751	The proper composite material	723:751	The proper composite material	723:751	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	7	1	dep	highlighted	1232:1242	arg1	studying					1247:1254	studying	1247:1254	studying their adsorbent properties	1247:1281	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	7	1	dep	highlighted	1232:1242	arg1	determining					1290:1300	determining	1290:1300	determining the maximum adsorption capacity for arsenic removal from aqueous solutions	1290:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	1	2	theme	paper	138:142	arg1	aim					126:128	The aim	122:128	The aim of this paper	122:142	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	2	3	theme	thermal	316:322	arg1	decomposition					324:336	simple thermal decomposition	309:336	simple thermal decomposition of a reaction mass obtained from starch and iron chloride	309:394	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	6	4	theme	X-Ray	1070:1074	arg1	Diffraction					1076:1086	X-Ray Diffraction	1070:1086	X-Ray Diffraction (XRD)	1070:1092	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	6	4	theme	X-Ray	1070:1074	arg1	XRD					1089:1091	XRD	1089:1091	XRD	1089:1091	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	7	5	theme	arsenic	1338:1344	arg1	removal					1346:1352	arsenic removal	1338:1352	arsenic removal from aqueous solutions	1338:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	4	6	theme	agent	716:720	arg1	addition					686:693	addition	686:693	addition of any precipitation agent	686:720	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	4	7	theme	precipitation	702:714	arg1	agent					716:720	any precipitation agent	698:720	any precipitation agent	698:720	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	5	8	theme	thermal	911:917	arg1	regime					919:924	the thermal regime	907:924	the thermal regime	907:924	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	1	9	theme	soluble	231:237	arg1	starch					239:244	soluble starch	231:244	soluble starch	231:244	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	0	10	theme	Oxide	105:109	arg1	Particles					111:119	Oxide Particles	105:119	Oxide Particles	105:119	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	3	11	theme	carbon	438:443	arg1	source					445:450	a carbon source	436:450	a carbon source	436:450	Starch used as a carbon source also efficiently stabilizes the iron oxides particles obtained during the thermal decomposition.
30935127	3	12	theme	iron	484:487	arg1	particles					496:504	the iron oxides particles	480:504	the iron oxides particles obtained during the thermal decomposition	480:546	Starch used as a carbon source also efficiently stabilizes the iron oxides particles obtained during the thermal decomposition.
30935127	4	13	theme	reaction	553:560	arg1	mass					562:565	The reaction mass	549:565	The reaction mass used for the thermal decomposition	549:600	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	0	14	dep	Carbon	89:94	arg1	Particles					111:119	Oxide Particles	105:119	Oxide Particles	105:119	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	6	15	theme	thermogravimetric	1042:1058	arg1	analysis					1060:1067	thermogravimetric analysis	1042:1067	thermogravimetric analysis	1042:1067	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	2	16	theme	inert	403:407	arg1	atmosphere					409:418	an inert atmosphere	400:418	an inert atmosphere	400:418	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	3	17	theme	thermal	526:532	arg1	decomposition					534:546	the thermal decomposition	522:546	the thermal decomposition	522:546	Starch used as a carbon source also efficiently stabilizes the iron oxides particles obtained during the thermal decomposition.
30935127	2	18	theme	mass	352:355	arg1	decomposition					324:336	simple thermal decomposition	309:336	simple thermal decomposition of a reaction mass obtained from starch and iron chloride	309:394	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	5	19	theme	adhering	783:790	arg1	quantity					837:844	rigorously adhering to the stirring time, temperature, and water quantity	772:844	rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition	772:992	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	2	20	theme	composite	271:279	arg1	material					281:288	The composite material	267:288	The composite material	267:288	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	2	21	theme	reaction	343:350	arg1	mass					352:355	a reaction mass	341:355	a reaction mass obtained from starch and iron chloride	341:394	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	7	22	theme	maximum	1306:1312	arg1	capacity					1325:1332	the maximum adsorption capacity	1302:1332	the maximum adsorption capacity for arsenic removal from aqueous solutions	1302:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	1	23	theme	composite	165:173	arg1	material					175:182	a new composite material	159:182	a new composite material based on carbon and iron oxides	159:214	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	1	24	theme	ferric	250:255	arg1	chloride					257:264	ferric chloride	250:264	ferric chloride	250:264	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	5	25	theme	regime	919:924	arg1	preparation					862:872	the preparation	858:872	the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition	858:992	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	4	26	theme	oxide	660:664	arg1	precursors					666:675	the carbon and iron oxide precursors	640:675	precursors	666:675	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	2	27	theme	iron	382:385	arg1	chloride					387:394	iron chloride	382:394	iron chloride	382:394	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	6	28	dep	Fourier	1135:1141	arg1	transform					1143:1151	transform	1143:1151	transform infra-red spectroscopy	1143:1174	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	1	29	theme	new	161:163	arg1	material					175:182	a new composite material	159:182	a new composite material based on carbon and iron oxides	159:214	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	6	30	theme	Synthesized	995:1005	arg1	materials					1007:1015	Synthesized materials	995:1015	Synthesized materials	995:1015	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	0	31	theme	Adsorptive	32:41	arg1	Performances					43:54	Adsorptive Performances	32:54	Adsorptive Performances	32:54	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	4	32	theme	thermal	580:586	arg1	decomposition					588:600	the thermal decomposition	576:600	the thermal decomposition	576:600	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	5	33	theme	proper	727:732	arg1	material					744:751	The proper composite material	723:751	The proper composite material	723:751	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	5	34	theme	thermal	972:978	arg1	decomposition					980:992	the thermal decomposition	968:992	the thermal decomposition	968:992	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	2	35	theme	simple	309:314	arg1	decomposition					324:336	simple thermal decomposition	309:336	simple thermal decomposition of a reaction mass obtained from starch and iron chloride	309:394	The composite material was synthesized by simple thermal decomposition of a reaction mass obtained from starch and iron chloride, in an inert atmosphere.
30935127	7	36	from	solutions	1367:1375	arg1	removal					1346:1352	arsenic removal	1338:1352	arsenic removal from aqueous solutions	1338:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	7	37	theme	aqueous	1359:1365	arg1	solutions					1367:1375	aqueous solutions	1359:1375	aqueous solutions	1359:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	5	38	theme	reaction	881:888	arg1	mass					890:893	the reaction mass	877:893	the reaction mass	877:893	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	3	39	theme	oxides	489:494	arg1	particles					496:504	the iron oxides particles	480:504	the iron oxides particles obtained during the thermal decomposition	480:546	Starch used as a carbon source also efficiently stabilizes the iron oxides particles obtained during the thermal decomposition.
30935127	5	40	theme	controlled	934:943	arg1	atmosphere					945:954	the controlled atmosphere	930:954	the controlled atmosphere	930:954	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	5	41	theme	mass	890:893	arg1	preparation					862:872	the preparation	858:872	the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition	858:992	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	6	42	theme	scanning	1095:1102	arg1	SEM					1125:1127	SEM	1125:1127	SEM	1125:1127	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	6	42	theme	scanning	1095:1102	arg1	microscopy					1113:1122	scanning electron microscopy	1095:1122	scanning electron microscopy (SEM)	1095:1128	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	7	43	theme	material	1218:1225	arg1	performances					1189:1200	The performances	1185:1200	The performances of the obtained material	1185:1225	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	0	44	theme	Material	71:78	arg1	Characterization					11:26	Characterization	11:26	Characterization	11:26	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	0	44	theme	Material	71:78	arg1	Performances					43:54	Adsorptive Performances	32:54	Adsorptive Performances	32:54	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	0	44	theme	Material	71:78	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	6	45	theme	infra-red	1153:1161	arg1	spectroscopy					1163:1174	infra-red spectroscopy	1153:1174	infra-red spectroscopy	1153:1174	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	5	46	theme	stirring	799:806	arg1	time					808:811	the stirring time	795:811	the stirring time	795:811	The proper composite material can be obtained by rigorously adhering to the stirring time, temperature, and water quantity used during the preparation of the reaction mass, as well as the thermal regime and the controlled atmosphere used during the thermal decomposition.
30935127	4	47	theme	iron	655:658	arg1	precursors					666:675	the carbon and iron oxide precursors	640:675	precursors	666:675	The reaction mass used for the thermal decomposition was obtained by simultaneously mixing the carbon and iron oxide precursors, without addition of any precipitation agent.
30935127	7	48	theme	adsorbent	1262:1270	arg1	properties					1272:1281	their adsorbent properties	1256:1281	their adsorbent properties	1256:1281	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	1	49	theme	iron	204:207	arg1	oxides					209:214	iron oxides	204:214	iron oxides	204:214	The aim of this paper was to produce a new composite material based on carbon and iron oxides, starting from soluble starch and ferric chloride.
30935127	0	50	theme	Composite	61:69	arg1	Material					71:78	a Composite Material	59:78	a Composite Material	59:78	Synthesis, Characterization and Adsorptive Performances of a Composite Material Based on Carbon and Iron Oxide Particles.
30935127	6	51	theme	electron	1104:1111	arg1	SEM					1125:1127	SEM	1125:1127	SEM	1125:1127	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	6	51	theme	electron	1104:1111	arg1	microscopy					1113:1122	scanning electron microscopy	1095:1122	scanning electron microscopy (SEM)	1095:1128	Synthesized materials were characterized using thermogravimetric analysis, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infra-red spectroscopy (FT-IR).
30935127	7	52	theme	adsorption	1314:1323	arg1	capacity					1325:1332	the maximum adsorption capacity	1302:1332	the maximum adsorption capacity for arsenic removal from aqueous solutions	1302:1375	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
30935127	7	53	theme	obtained	1209:1216	arg1	material					1218:1225	the obtained material	1205:1225	the obtained material	1205:1225	The performances of the obtained material were highlighted by studying their adsorbent properties and by determining the maximum adsorption capacity for arsenic removal from aqueous solutions.
31731566	7	0	theme	FT-IR	975:979	arg1	spectroscopy					982:993	InfraRed (FT-IR) spectroscopy	965:993	InfraRed (FT-IR) spectroscopy	965:993	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	2	1	theme	friendly	366:373	arg1	processes					375:383	environmentally friendly processes	350:383	environmentally friendly processes	350:383	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	2	2	with	curing	301:306	arg1	processes					375:383	environmentally friendly processes	350:383	environmentally friendly processes	350:383	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	2	3	theme	environmentally	350:364	arg1	processes					375:383	environmentally friendly processes	350:383	environmentally friendly processes	350:383	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	8	4	theme	thermogravimetric	1153:1169	arg1	analysis					1171:1178	thermogravimetric analysis	1153:1178	thermogravimetric analysis	1153:1178	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	7	5	theme	InfraRed	965:972	arg1	spectroscopy					982:993	InfraRed (FT-IR) spectroscopy	965:993	InfraRed (FT-IR) spectroscopy	965:993	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	5	6	theme	MFC	801:803	arg1	mats					805:808	Wet MFC mats	797:808	Wet MFC mats	797:808	Wet MFC mats were prepared by filtration, and then impregnated with the resin.
31731566	1	7	dep	Liquid	166:171	arg1	CNSL					174:177	CNSL	174:177	CNSL	174:177	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	3	8	theme	room	508:511	arg1	operation					525:533	room temperature operation	508:533	room temperature operation with high reaction rates	508:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	4	9	with	combination	697:707	arg1	cellulose					731:739	microfibrillated cellulose	714:739	microfibrillated cellulose (MFC)	714:745	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	4	9	with	combination	697:707	arg1	MFC					742:744	MFC	742:744	MFC	742:744	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	7	10	theme	composites	1074:1083	arg1	photocuring					1023:1033	the photocuring	1019:1033	the photocuring of the epoxidized cardanol, and of the composites	1019:1083	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	2	11	theme	sustainable	274:284	arg1	materials					286:294	sustainable materials	274:294	sustainable materials	274:294	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	8	12	theme	mechanical	1228:1237	arg1	analysis					1239:1246	dynamic mechanical analysis	1220:1246	dynamic mechanical analysis	1220:1246	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	6	13	theme	impregnated	880:890	arg1	mats					892:895	The impregnated mats	876:895	The impregnated mats	876:895	The impregnated mats were then irradiated with ultraviolet (UV) light.
31731566	6	13	theme	impregnated	880:890	arg1	irradiated					907:916	irradiated	907:916	irradiated	907:916	The impregnated mats were then irradiated with ultraviolet (UV) light.
31731566	8	14	theme	dynamic	1220:1226	arg1	analysis					1239:1246	dynamic mechanical analysis	1220:1246	dynamic mechanical analysis	1220:1246	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	4	15	theme	biobased	776:783	arg1	composites					785:794	fully biobased composites	770:794	fully biobased composites	770:794	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	4	16	from	use	690:692	arg1	combination					697:707	combination	697:707	combination with microfibrillated cellulose (MFC)	697:745	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	8	17	theme	scanning	1194:1201	arg1	calorimetry					1203:1213	differential scanning calorimetry	1181:1213	differential scanning calorimetry	1181:1213	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	2	18	theme	biobased	311:318	arg1	monomers					320:327	biobased monomers	311:327	biobased monomers	311:327	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	9	19	theme	high	1320:1323	arg1	concentration					1340:1352	a high photoinitiator concentration	1318:1352	a high photoinitiator concentration	1318:1352	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
31731566	8	20	theme	differential	1181:1192	arg1	calorimetry					1203:1213	differential scanning calorimetry	1181:1213	differential scanning calorimetry	1181:1213	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	3	21	with	operation	525:533	arg1	rates					554:558	high reaction rates	540:558	high reaction rates	540:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	1	22	theme	non-food	183:190	arg1	NutShell					157:164	NutShell	157:164	NutShell	157:164	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	22	theme	non-food	183:190	arg1	material					215:222	a non-food annually renewable raw material	181:222	a non-food annually renewable raw material extracted from cashew nutshells	181:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	0	23	theme	Cardanol	26:33	arg1	Photocuring					0:10	Photocuring	0:10	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.	0:90	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.
31731566	1	24	theme	natural	106:112	arg1	compound					128:135	a natural alkylphenolic compound	104:135	a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells	104:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	24	theme	natural	106:112	arg1	Cardanol					92:99	Cardanol	92:99	Cardanol	92:99	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	3	25	theme	energy	487:492	arg1	requirements					494:505	low energy requirements	483:505	low energy requirements	483:505	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	0	26	theme	Epoxidized	15:24	arg1	Cardanol					26:33	Epoxidized Cardanol	15:33	Epoxidized Cardanol	15:33	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.
31731566	4	27	theme	composites	785:794	arg1	fabrication					755:765	the fabrication	751:765	the fabrication of fully biobased composites	751:794	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	1	28	theme	alkylphenolic	114:126	arg1	compound					128:135	a natural alkylphenolic compound	104:135	a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells	104:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	28	theme	alkylphenolic	114:126	arg1	Cardanol					92:99	Cardanol	92:99	Cardanol	92:99	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	0	29	theme	Biobased	39:46	arg1	Composites					48:57	Biobased Composites	39:57	Biobased Composites with Microfibrillated Cellulose	39:89	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.
31731566	4	30	theme	microfibrillated	714:729	arg1	cellulose					731:739	microfibrillated cellulose	714:739	microfibrillated cellulose (MFC)	714:745	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	4	30	theme	microfibrillated	714:729	arg1	MFC					742:744	MFC	742:744	MFC	742:744	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	7	31	theme	epoxidized	1042:1051	arg1	cardanol					1053:1060	the epoxidized cardanol	1038:1060	the epoxidized cardanol	1038:1060	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	4	32	theme	cardanol	664:671	arg1	photocuring					613:623	the photocuring	609:623	the photocuring of a commercially available epoxidized cardanol	609:671	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	3	33	theme	green	457:461	arg1	technology					463:472	a green technology	455:472	a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents	455:583	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	3	33	theme	green	457:461	arg1	Photopolymerization					415:433	Photopolymerization	415:433	Photopolymerization	415:433	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	1	34	theme	raw	211:213	arg1	NutShell					157:164	NutShell	157:164	NutShell	157:164	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	34	theme	raw	211:213	arg1	material					215:222	a non-food annually renewable raw material	181:222	a non-food annually renewable raw material extracted from cashew nutshells	181:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	5	35	theme	Wet	797:799	arg1	mats					805:808	Wet MFC mats	797:808	Wet MFC mats	797:808	Wet MFC mats were prepared by filtration, and then impregnated with the resin.
31731566	3	36	with	requirements	494:505	arg1	rates					554:558	high reaction rates	540:558	high reaction rates	540:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	6	37	with	irradiated	907:916	arg1	light					940:944	ultraviolet (UV) light	923:944	ultraviolet (UV) light	923:944	The impregnated mats were then irradiated with ultraviolet (UV) light.
31731566	1	38	dep	non-food	183:190	arg1	renewable					201:209	renewable	201:209	renewable	201:209	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	3	39	theme	low	483:485	arg1	requirements					494:505	low energy requirements	483:505	low energy requirements	483:505	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	3	40	with	absence	565:571	arg1	rates					554:558	high reaction rates	540:558	high reaction rates	540:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	8	41	theme	thermomechanical	1090:1105	arg1	properties					1107:1116	The thermomechanical properties	1086:1116	The thermomechanical properties of the composites	1086:1134	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	9	42	theme	photoinitiator	1325:1338	arg1	concentration					1340:1352	a high photoinitiator concentration	1318:1352	a high photoinitiator concentration	1318:1352	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
31731566	3	43	theme	temperature	513:523	arg1	operation					525:533	room temperature operation	508:533	room temperature operation with high reaction rates	508:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	7	44	theme	cardanol	1053:1060	arg1	photocuring					1023:1033	the photocuring	1019:1033	the photocuring of the epoxidized cardanol, and of the composites	1019:1083	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	6	45	theme	UV	936:937	arg1	light					940:944	ultraviolet (UV) light	923:944	ultraviolet (UV) light	923:944	The impregnated mats were then irradiated with ultraviolet (UV) light.
31731566	6	46	theme	ultraviolet	923:933	arg1	light					940:944	ultraviolet (UV) light	923:944	ultraviolet (UV) light	923:944	The impregnated mats were then irradiated with ultraviolet (UV) light.
31731566	3	47	theme	high	540:543	arg1	rates					554:558	high reaction rates	540:558	high reaction rates	540:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	2	48	theme	prepolymers	333:343	arg1	curing					301:306	the curing	297:306	the curing of biobased monomers and prepolymers with environmentally friendly processes	297:383	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	7	49	used	used	999:1002	arg2	Fourier					947:953	Fourier	947:953	Fourier Transform InfraRed (FT-IR) spectroscopy	947:993	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	9	50	theme	side	1384:1387	arg1	reaction					1389:1396	a side reaction	1382:1396	a side reaction of the photoinitiator with MFC	1382:1427	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
31731566	9	51	theme	cured	1273:1277	arg1	composites					1279:1288	fully cured composites	1267:1288	fully cured composites	1267:1288	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
31731566	3	52	theme	solvents	576:583	arg1	absence					565:571	absence	565:571	absence of solvents	565:583	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	3	52	theme	solvents	576:583	arg1	operation					525:533	room temperature operation	508:533	room temperature operation with high reaction rates	508:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	3	52	theme	solvents	576:583	arg1	requirements					494:505	low energy requirements	483:505	low energy requirements	483:505	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	2	53	theme	monomers	320:327	arg1	curing					301:306	the curing	297:306	the curing of biobased monomers and prepolymers with environmentally friendly processes	297:383	In the quest for sustainable materials, the curing of biobased monomers and prepolymers with environmentally friendly processes attracts increasing interest.
31731566	8	54	theme	composites	1125:1134	arg1	properties					1107:1116	The thermomechanical properties	1086:1116	The thermomechanical properties of the composites	1086:1134	The thermomechanical properties of the composites were assessed by thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis.
31731566	4	55	theme	epoxidized	653:662	arg1	cardanol					664:671	a commercially available epoxidized cardanol	628:671	a commercially available epoxidized cardanol	628:671	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	0	56	with	Composites	48:57	arg1	Cellulose					81:89	Microfibrillated Cellulose	64:89	Microfibrillated Cellulose	64:89	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.
31731566	3	57	theme	reaction	545:552	arg1	rates					554:558	high reaction rates	540:558	high reaction rates	540:558	Photopolymerization is considered to be a green technology owing to low energy requirements, room temperature operation with high reaction rates, and absence of solvents.
31731566	7	58	dep	Fourier	947:953	arg1	Transform					955:963	Transform	955:963	Transform InfraRed (FT-IR) spectroscopy	955:993	Fourier Transform InfraRed (FT-IR) spectroscopy was used to investigate the photocuring of the epoxidized cardanol, and of the composites.
31731566	1	59	theme	cashew	239:244	arg1	nutshells					246:254	cashew nutshells	239:254	cashew nutshells	239:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	0	60	theme	Microfibrillated	64:79	arg1	Cellulose					81:89	Microfibrillated Cellulose	64:89	Microfibrillated Cellulose	64:89	Photocuring of Epoxidized Cardanol for Biobased Composites with Microfibrillated Cellulose.
31731566	1	61	attach	derived	137:143	arg2	compound					128:135	a natural alkylphenolic compound	104:135	a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells	104:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	61	attach	derived	137:143	arg2	Cardanol					92:99	Cardanol	92:99	Cardanol	92:99	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	61	attach	derived	137:143	arg1	NutShell					157:164	NutShell	157:164	NutShell	157:164	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	1	61	attach	derived	137:143	arg1	material					215:222	a non-food annually renewable raw material	181:222	a non-food annually renewable raw material extracted from cashew nutshells	181:254	Cardanol is a natural alkylphenolic compound derived from Cashew NutShell Liquid (CNSL), a non-food annually renewable raw material extracted from cashew nutshells.
31731566	4	62	theme	available	643:651	arg1	cardanol					664:671	a commercially available epoxidized cardanol	628:671	a commercially available epoxidized cardanol	628:671	In this work, we study the photocuring of a commercially available epoxidized cardanol, and explore its use in combination with microfibrillated cellulose (MFC) for the fabrication of fully biobased composites.
31731566	9	63	with	reaction	1389:1396	arg1	MFC					1425:1427	MFC	1425:1427	MFC	1425:1427	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
31731566	9	64	theme	photoinitiator	1405:1418	arg1	reaction					1389:1396	a side reaction	1382:1396	a side reaction of the photoinitiator with MFC	1382:1427	We confirmed that fully cured composites could be obtained, although a high photoinitiator concentration was needed, possibly due to a side reaction of the photoinitiator with MFC.
29969639	0	0	from	study	8:12	arg1	scaffolds					17:25	scaffolds	17:25	scaffolds	17:25	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	6	1	theme	hydroxyapatite	709:722	arg1	addition					697:704	The addition	693:704	The addition of hydroxyapatite	693:722	The addition of hydroxyapatite delayed the degradation process of the obtained scaffolds.
29969639	9	2	theme	%	1029:1029	arg1	wt					1031:1032	80% wt	1027:1032	80% wt	1027:1032	However, the scaffolds supplemented with 80% wt.
29969639	4	3	theme	in	502:503	arg1	implantation					510:521	in vivo implantation	502:521	in vivo implantation	502:521	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	7	4	theme	wt	940:941	arg1	addition					924:931	addition	924:931	addition of 50% wt	924:941	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	7	5	theme	control	863:869	arg1	scaffold					871:878	the control scaffold	859:878	the control scaffold without hydroxyapatite (HAp)	859:907	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	1	6	theme	freeze-drying	228:240	arg1	method					242:247	the freeze-drying method	224:247	the freeze-drying method	224:247	Scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with nano-hydroxyapatite were obtained with the use of the freeze-drying method.
29969639	7	7	theme	50	936:937	arg1	%					938:938	%	938:938	%	938:938	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	7	8	dep	showed	841:846	arg1	those					913:917	those	913:917	those	913:917	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	7	8	dep	showed	841:846	arg1	scaffold					871:878	the control scaffold	859:878	the control scaffold without hydroxyapatite (HAp)	859:907	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	1	9	theme	method	242:247	arg1	use					217:219	the use	213:219	the use of the freeze-drying method	213:247	Scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with nano-hydroxyapatite were obtained with the use of the freeze-drying method.
29969639	0	10	theme	hyaluronic	60:69	arg1	acid					71:74	hyaluronic acid	60:74	hyaluronic acid	60:74	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	4	11	theme	chosen	462:467	arg1	scaffolds					469:477	the chosen scaffolds	458:477	the chosen scaffolds	458:477	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	5	12	theme	hyaluronic	643:652	arg1	acid					654:657	hyaluronic acid	643:657	hyaluronic acid	643:657	The results showed low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite.
29969639	0	13	with	collagen	46:53	arg1	hydroxyapatite					81:94	hydroxyapatite	81:94	hydroxyapatite	81:94	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	10	14	theme	implanted	1056:1064	arg1	place					1066:1070	the implanted place	1052:1070	the implanted place	1052:1070	HAp premained in the implanted place.
29969639	5	15	theme	scaffolds	600:608	arg1	stability					583:591	low stability	579:591	low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite	579:690	The results showed low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite.
29969639	3	16	theme	4-day	419:423	arg1	culture					425:431	4-day culture	419:431	4-day culture	419:431	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	0	17	theme	In	0:1	arg1	study					8:12	In vivo study	0:12	In vivo study on scaffolds	0:25	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	1	18	theme	hyaluronic	140:149	arg1	acid					151:154	hyaluronic acid	140:154	hyaluronic acid	140:154	Scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with nano-hydroxyapatite were obtained with the use of the freeze-drying method.
29969639	3	19	theme	SaOS-2	372:377	arg1	cells					379:383	human osteosarcoma SaOS-2 cells	353:383	human osteosarcoma SaOS-2 cells	353:383	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	4	20	theme	scaffolds	469:477	arg1	biocompatibility					438:453	The biocompatibility	434:453	The biocompatibility of the chosen scaffolds	434:477	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	2	21	theme	liquid	299:304	arg1	test					313:316	the liquid uptake test	295:316	the liquid uptake test	295:316	Composites swelling behavior was assessed by the liquid uptake test.
29969639	3	22	theme	cells	379:383	arg1	proliferation					336:348	proliferation	336:348	proliferation	336:348	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	3	22	theme	cells	379:383	arg1	adhesion					323:330	adhesion	323:330	adhesion	323:330	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	0	23	with	acid	71:74	arg1	hydroxyapatite					81:94	hydroxyapatite	81:94	hydroxyapatite	81:94	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	3	24	theme	human	353:357	arg1	cells					379:383	human osteosarcoma SaOS-2 cells	353:383	human osteosarcoma SaOS-2 cells	353:383	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	6	25	theme	degradation	736:746	arg1	process					748:754	the degradation process	732:754	the degradation process of the obtained scaffolds	732:780	The addition of hydroxyapatite delayed the degradation process of the obtained scaffolds.
29969639	5	26	theme	low	579:581	arg1	stability					583:591	low stability	579:591	low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite	579:690	The results showed low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite.
29969639	3	27	theme	osteosarcoma	359:370	arg1	cells					379:383	human osteosarcoma SaOS-2 cells	353:383	human osteosarcoma SaOS-2 cells	353:383	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	3	28	from	adhesion	323:330	arg1	scaffolds					392:400	the scaffolds	388:400	the scaffolds	388:400	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	7	29	theme	%	938:938	arg1	wt					940:941	50% wt	936:941	50% wt	936:941	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	4	30	theme	rabbits	551:557	arg1	tissue					541:546	subcutaneous tissue	528:546	subcutaneous tissue of rabbits	528:557	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	11	31	theme	satisfactory	1092:1103	arg1	response					1112:1119	satisfactory tissue response	1092:1119	satisfactory tissue response	1092:1119	The results showed satisfactory tissue response on the implanted scaffolds.
29969639	4	32	dep	in	502:503	arg1	vivo					505:508	vivo	505:508	vivo	505:508	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	3	33	from	proliferation	336:348	arg1	scaffolds					392:400	the scaffolds	388:400	the scaffolds	388:400	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	11	34	theme	tissue	1105:1110	arg1	response					1112:1119	satisfactory tissue response	1092:1119	satisfactory tissue response	1092:1119	The results showed satisfactory tissue response on the implanted scaffolds.
29969639	6	35	theme	obtained	763:770	arg1	scaffolds					772:780	the obtained scaffolds	759:780	the obtained scaffolds	759:780	The addition of hydroxyapatite delayed the degradation process of the obtained scaffolds.
29969639	7	36	theme	X-ray	787:791	arg1	images					793:798	The X-ray images	783:798	The X-ray images of the tissues surrounding the scaffolds	783:839	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	3	37	dep	adhesion	323:330	arg1	The					319:321	The	319:321	The	319:321	The adhesion and proliferation of human osteosarcoma SaOS-2 cells on the scaffolds were examined in 4-day culture.
29969639	7	38	theme	tissues	807:813	arg1	images					793:798	The X-ray images	783:798	The X-ray images of the tissues surrounding the scaffolds	783:839	The X-ray images of the tissues surrounding the scaffolds showed that both, the control scaffold without hydroxyapatite (HAp) and those with addition of 50% wt.
29969639	4	39	theme	subcutaneous	528:539	arg1	tissue					541:546	subcutaneous tissue	528:546	subcutaneous tissue of rabbits	528:557	The biocompatibility of the chosen scaffolds was further studied by in vivo implantation into subcutaneous tissue of rabbits.
29969639	6	40	theme	scaffolds	772:780	arg1	process					748:754	the degradation process	732:754	the degradation process of the obtained scaffolds	732:780	The addition of hydroxyapatite delayed the degradation process of the obtained scaffolds.
29969639	11	41	theme	implanted	1128:1136	arg1	scaffolds					1138:1146	the implanted scaffolds	1124:1146	the implanted scaffolds	1124:1146	The results showed satisfactory tissue response on the implanted scaffolds.
29969639	0	42	with	chitosan	36:43	arg1	hydroxyapatite					81:94	hydroxyapatite	81:94	hydroxyapatite	81:94	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	2	43	theme	uptake	306:311	arg1	test					313:316	the liquid uptake test	295:316	the liquid uptake test	295:316	Composites swelling behavior was assessed by the liquid uptake test.
29969639	0	44	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo study on scaffolds based on chitosan, collagen, and hyaluronic acid with hydroxyapatite.
29969639	9	45	theme	80	1027:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	However, the scaffolds supplemented with 80% wt.
31523007	5	0	theme	polysaccharide	726:739	arg1	utilization					741:751	polysaccharide utilization	726:751	polysaccharide utilization	726:751	Notably, Bacteroides thetaiotaomicron, which blooms in response to amoxicillin, upregulated polysaccharide utilization.
31523007	6	1	theme	bacterium	802:810	arg1	sensitivity					782:792	the sensitivity	778:792	the sensitivity of this bacterium to amoxicillin	778:825	In vitro, we found that the sensitivity of this bacterium to amoxicillin was elevated by glucose and reduced by polysaccharides.
31523007	4	2	theme	pathways	575:582	arg1	expression					547:556	the expression	543:556	the expression of key metabolic pathways at the whole-community and single-species levels	543:631	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	8	3	theme	microbiome	1116:1125	arg1	environment					1097:1107	the metabolic environment	1083:1107	the metabolic environment of the microbiome	1083:1125	Our work indicates that the metabolic environment of the microbiome plays a role in the response of this community to antibiotics.
31523007	3	4	theme	metatranscriptomic	372:389	arg1	approach					391:398	a metagenomic and metatranscriptomic approach	354:398	a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome	354:494	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	4	5	theme	metabolic	565:573	arg1	pathways					575:582	key metabolic pathways	561:582	key metabolic pathways	561:582	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	2	6	theme	large	315:319	arg1	role					321:324	a large role	313:324	a large role	313:324	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	7	7	theme	B.	973:974	arg1	thetaiotaomicron					976:991	B. thetaiotaomicron	973:991	B. thetaiotaomicron	973:991	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	3	8	theme	metagenomic	356:366	arg1	approach					391:398	a metagenomic and metatranscriptomic approach	354:398	a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome	354:494	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	8	9	theme	metabolic	1087:1095	arg1	environment					1097:1107	the metabolic environment	1083:1107	the metabolic environment of the microbiome	1083:1125	Our work indicates that the metabolic environment of the microbiome plays a role in the response of this community to antibiotics.
31523007	5	10	dep	amoxicillin	701:711	arg1	response					689:696	response	689:696	response	689:696	Notably, Bacteroides thetaiotaomicron, which blooms in response to amoxicillin, upregulated polysaccharide utilization.
31523007	7	11	theme	microbiome	1019:1028	arg1	disruption					1030:1039	microbiome disruption	1019:1039	microbiome disruption with amoxicillin	1019:1056	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	0	12	theme	Microbial	0:8	arg1	Metabolism					10:19	Microbial Metabolism	0:19	Microbial Metabolism	0:19	Microbial Metabolism Modulates Antibiotic Susceptibility within the Murine Gut Microbiome.
31523007	3	13	theme	antibiotic-induced	408:425	arg1	responses					457:465	antibiotic-induced taxonomic and transcriptional responses	408:465	antibiotic-induced taxonomic and transcriptional responses	408:465	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	5	14	theme	Bacteroides	643:653	arg1	thetaiotaomicron					655:670	Bacteroides thetaiotaomicron	643:670	Bacteroides thetaiotaomicron	643:670	Notably, Bacteroides thetaiotaomicron, which blooms in response to amoxicillin, upregulated polysaccharide utilization.
31523007	3	15	theme	taxonomic	427:435	arg1	responses					457:465	antibiotic-induced taxonomic and transcriptional responses	408:465	antibiotic-induced taxonomic and transcriptional responses	408:465	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	3	16	theme	murine	478:483	arg1	microbiome					485:494	the murine microbiome	474:494	the murine microbiome	474:494	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	0	17	theme	Antibiotic	31:40	arg1	Susceptibility					42:55	Antibiotic Susceptibility	31:55	Antibiotic Susceptibility	31:55	Microbial Metabolism Modulates Antibiotic Susceptibility within the Murine Gut Microbiome.
31523007	4	18	theme	single-species	611:624	arg1	levels					626:631	the whole-community and single-species levels	587:631	levels	626:631	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	8	19	theme	community	1164:1172	arg1	response					1147:1154	the response	1143:1154	the response of this community to antibiotics	1143:1187	Our work indicates that the metabolic environment of the microbiome plays a role in the response of this community to antibiotics.
31523007	7	20	with	disruption	1030:1039	arg1	amoxicillin					1046:1056	amoxicillin	1046:1056	amoxicillin	1046:1056	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	7	21	theme	dietary	913:919	arg1	composition					921:931	dietary composition	913:931	dietary composition	913:931	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	2	22	theme	important	249:257	arg1	regulator					259:267	an important regulator	246:267	an important regulator of susceptibility in vitro	246:294	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	2	22	theme	important	249:257	arg1	metabolism					232:241	Bacterial metabolism	222:241	Bacterial metabolism	222:241	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	4	23	from	levels	626:631	arg1	expression					547:556	the expression	543:556	the expression of key metabolic pathways at the whole-community and single-species levels	543:631	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	0	24	theme	Murine	68:73	arg1	Microbiome					79:88	the Murine Gut Microbiome	64:88	the Murine Gut Microbiome	64:88	Microbial Metabolism Modulates Antibiotic Susceptibility within the Murine Gut Microbiome.
31523007	7	25	dep	abundance	946:954	arg1	the					942:944	the	942:944	the	942:944	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	3	26	theme	transcriptional	441:455	arg1	responses					457:465	antibiotic-induced taxonomic and transcriptional responses	408:465	antibiotic-induced taxonomic and transcriptional responses	408:465	We applied a metagenomic and metatranscriptomic approach to link antibiotic-induced taxonomic and transcriptional responses within the murine microbiome.
31523007	7	27	theme	disruption	1030:1039	arg1	abundance					946:954	abundance	946:954	abundance	946:954	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	7	27	theme	disruption	1030:1039	arg1	extent					1009:1014	the extent	1005:1014	the extent of microbiome disruption with amoxicillin	1005:1056	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	7	27	theme	disruption	1030:1039	arg1	expansion					960:968	expansion	960:968	expansion	960:968	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	4	28	theme	whole-community	591:605	arg1	levels					626:631	the whole-community and single-species levels	587:631	levels	626:631	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	2	29	theme	Bacterial	222:230	arg1	regulator					259:267	an important regulator	246:267	an important regulator of susceptibility in vitro	246:294	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	2	29	theme	Bacterial	222:230	arg1	metabolism					232:241	Bacterial metabolism	222:241	Bacterial metabolism	222:241	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	1	30	theme	gut	141:143	arg1	microbiota					145:154	the gut microbiota	137:154	the gut microbiota	137:154	Although antibiotics disturb the structure of the gut microbiota, factors that modulate these perturbations are poorly understood.
31523007	2	31	theme	in vitro	287:294	arg1	regulator					259:267	an important regulator	246:267	an important regulator of susceptibility in vitro	246:294	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	2	31	theme	in vitro	287:294	arg1	metabolism					232:241	Bacterial metabolism	222:241	Bacterial metabolism	222:241	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	1	32	theme	microbiota	145:154	arg1	structure					124:132	the structure	120:132	the structure of the gut microbiota	120:154	Although antibiotics disturb the structure of the gut microbiota, factors that modulate these perturbations are poorly understood.
31523007	2	33	theme	susceptibility	272:285	arg1	in vitro					287:294	susceptibility in vitro	272:294	susceptibility in vitro	272:294	Bacterial metabolism is an important regulator of susceptibility in vitro and likely plays a large role within the host.
31523007	0	34	theme	Gut	75:77	arg1	Microbiome					79:88	the Murine Gut Microbiome	64:88	the Murine Gut Microbiome	64:88	Microbial Metabolism Modulates Antibiotic Susceptibility within the Murine Gut Microbiome.
31523007	4	35	theme	key	561:563	arg1	pathways					575:582	key metabolic pathways	561:582	key metabolic pathways	561:582	We found that antibiotics significantly alter the expression of key metabolic pathways at the whole-community and single-species levels.
31523007	7	36	theme	thetaiotaomicron	976:991	arg1	abundance					946:954	abundance	946:954	abundance	946:954	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	7	36	theme	thetaiotaomicron	976:991	arg1	extent					1009:1014	the extent	1005:1014	the extent of microbiome disruption with amoxicillin	1005:1056	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
31523007	7	36	theme	thetaiotaomicron	976:991	arg1	expansion					960:968	expansion	960:968	expansion	960:968	Accordingly, we observed that dietary composition affected the abundance and expansion of B. thetaiotaomicron, as well as the extent of microbiome disruption with amoxicillin.
29715500	6	0	theme	%	975:975	arg1	content					986:992	% moisture content	975:992	% moisture content	975:992	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	4	1	theme	current	590:596	arg1	study					598:602	the current study	586:602	the current study	586:602	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	6	2	theme	strength	880:887	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	2	3	theme	forming	365:371	arg1	Chitosan					312:319	Chitosan	312:319	Chitosan (CS)	312:324	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	2	3	theme	forming	365:371	arg1	material					373:380	a forming material	363:380	a forming material of different buccal dosage forms including sponges	363:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	6	4	theme	pH	971:972	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	1	5	theme	attractive	289:298	arg1	approaches					300:309	the most attractive approaches	280:309	the most attractive approaches	280:309	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	2	6	theme	dosage	402:407	arg1	forms					409:413	different buccal dosage forms	385:413	different buccal dosage forms including sponges	385:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	2	6	theme	dosage	402:407	arg1	sponges					425:431	sponges	425:431	sponges	425:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	11	7	theme	mucoadhesive	1641:1652	arg1	properties					1654:1663	mucoadhesive properties	1641:1663	mucoadhesive properties	1641:1663	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	1	8	theme	dosage	198:203	arg1	forms					205:209	buccal dosage forms	191:209	buccal dosage forms	191:209	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	4	9	theme	buccal	687:692	arg1	sponges					713:719	buccal CS-based composite sponges	687:719	buccal CS-based composite sponges	687:719	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	6	10	theme	proposed	828:835	arg1	sponges					837:843	the proposed sponges	824:843	the proposed sponges	824:843	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	6	11	theme	viscosity	1018:1026	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	7	12	theme	scanning	1127:1134	arg1	microscope					1145:1154	scanning electron microscope	1127:1154	scanning electron microscope	1127:1154	Furthermore, the surface topology of selected sponges was viewed using scanning electron microscope.
29715500	3	13	theme	poor	537:540	arg1	properties					550:559	poor tensile properties	537:559	poor tensile properties	537:559	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	6	14	theme	capacity	1008:1015	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	3	15	theme	buccal	452:457	arg1	systems					468:474	CS-based buccal delivery systems	443:474	CS-based buccal delivery systems	443:474	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	1	16	theme	transmucosal	236:247	arg1	delivery					254:261	transmucosal drug delivery	236:261	transmucosal drug delivery	236:261	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	6	17	theme	water	995:999	arg1	capacity					1008:1015	water uptake capacity	995:1015	water uptake capacity	995:1015	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	7	18	theme	sponges	1102:1108	arg1	topology					1081:1088	the surface topology	1069:1088	the surface topology of selected sponges	1069:1108	Furthermore, the surface topology of selected sponges was viewed using scanning electron microscope.
29715500	6	19	theme	content	986:992	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	11	20	theme	soft	1624:1627	arg1	texture					1629:1635	their soft texture	1618:1635	their soft texture	1618:1635	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	4	21	theme	composite	703:711	arg1	sponges					713:719	buccal CS-based composite sponges	687:719	buccal CS-based composite sponges	687:719	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	0	22	theme	chitosan-based	63:76	arg1	sponges					88:94	buccal chitosan-based composite sponges	56:94	buccal chitosan-based composite sponges	56:94	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	9	23	theme	physicochemical	1316:1330	arg1	characteristics					1332:1346	the best physicochemical characteristics	1307:1346	the best physicochemical characteristics suitable for buccal applications	1307:1379	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	11	24	theme	humidity	1589:1596	arg1	conditions					1598:1607	low humidity conditions	1585:1607	low humidity conditions	1585:1607	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	0	25	theme	sponges	88:94	arg1	fabrication					41:51	fabrication	41:51	fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics	41:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	9	26	theme	suitable	1348:1355	arg1	characteristics					1332:1346	the best physicochemical characteristics	1307:1346	the best physicochemical characteristics suitable for buccal applications	1307:1379	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	9	27	dep	sponges	1264:1270	arg1	1:1					1295:1297	1:1	1295:1297	1:1	1295:1297	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	9	27	dep	sponges	1264:1270	arg1	HPMC					1289:1292	HPMC	1289:1292	sponges fabricated of CS:HPMC (1:1)	1264:1298	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	0	28	theme	augmented	101:109	arg1	characteristics					124:138	augmented mucoadhesive characteristics	101:138	augmented mucoadhesive characteristics	101:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	9	29	theme	buccal	1361:1366	arg1	applications					1368:1379	buccal applications	1361:1379	buccal applications	1361:1379	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	6	30	theme	%	1032:1032	arg1	porosity					1034:1041	% porosity	1032:1041	% porosity	1032:1041	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	3	31	theme	delivery	459:466	arg1	systems					468:474	CS-based buccal delivery systems	443:474	CS-based buccal delivery systems	443:474	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	3	32	theme	CS-based	443:450	arg1	systems					468:474	CS-based buccal delivery systems	443:474	CS-based buccal delivery systems	443:474	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	1	33	theme	approaches	300:309	arg1	approaches					300:309	the most attractive approaches	280:309	the most attractive approaches	280:309	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	1	33	theme	approaches	300:309	arg1	one					273:275	one	273:275	one	273:275	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	6	34	theme	mechanical	869:878	arg1	strength					880:887	mechanical strength determination	869:901	mechanical strength determination	869:901	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	3	35	theme	many	488:491	arg1	limitations					493:503	many limitations	488:503	many limitations like weak adhesion strength and poor tensile properties	488:559	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	0	36	theme	blending	19:26	arg1	approach					28:35	polymer blending approach	11:35	polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics	11:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	6	37	dep	Fourier	904:910	arg1	transform					912:920	transform	912:920	transform infrared spectroscopic analysis (FT-IR)	912:960	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	11	38	theme	stability	1536:1544	arg1	study					1546:1550	Preliminary stability study	1524:1550	Preliminary stability study	1524:1550	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	6	39	theme	spectroscopic	931:943	arg1	FT-IR					955:959	FT-IR	955:959	FT-IR	955:959	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	6	39	theme	spectroscopic	931:943	arg1	analysis					945:952	spectroscopic analysis	931:952	spectroscopic analysis (FT-IR)	931:960	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	3	40	theme	adhesion	515:522	arg1	strength					524:531	weak adhesion strength	510:531	weak adhesion strength	510:531	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	8	41	theme	mucoadhesive	1161:1172	arg1	properties					1174:1183	The mucoadhesive properties	1157:1183	The mucoadhesive properties of sponges	1157:1194	The mucoadhesive properties of sponges were tested both ex-vivo and in-vivo.
29715500	6	42	theme	Thorough	786:793	arg1	characterization					804:819	Thorough in-vitro characterization	786:819	Thorough in-vitro characterization of the proposed sponges	786:843	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	1	43	theme	drug	249:252	arg1	delivery					254:261	transmucosal drug delivery	236:261	transmucosal drug delivery	236:261	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	3	44	theme	tensile	542:548	arg1	properties					550:559	poor tensile properties	537:559	poor tensile properties	537:559	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	6	45	theme	moisture	977:984	arg1	content					986:992	% moisture content	975:992	% moisture content	975:992	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	4	46	theme	polymer	619:625	arg1	approach					636:643	the polymer blending approach	615:643	the polymer blending approach	615:643	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	9	47	theme	fabricated	1244:1253	arg1	sponges					1255:1261	all fabricated sponges	1240:1261	all fabricated sponges	1240:1261	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	6	48	theme	surface	963:969	arg1	pH					971:972	surface pH	963:972	surface pH	963:972	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	1	49	theme	buccal	191:196	arg1	forms					205:209	buccal dosage forms	191:209	buccal dosage forms	191:209	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	4	50	theme	mucoadhesive	660:671	arg1	properties					673:682	the mucoadhesive properties	656:682	the mucoadhesive properties of buccal CS-based composite sponges	656:719	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	7	51	theme	electron	1136:1143	arg1	microscope					1145:1154	scanning electron microscope	1127:1154	scanning electron microscope	1127:1154	Furthermore, the surface topology of selected sponges was viewed using scanning electron microscope.
29715500	6	52	theme	sponges	837:843	arg1	characterization					804:819	Thorough in-vitro characterization	786:819	Thorough in-vitro characterization of the proposed sponges	786:843	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	1	53	theme	forms	205:209	arg1	modernization					174:186	modernization	174:186	modernization	174:186	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	1	53	theme	forms	205:209	arg1	application					158:168	application	158:168	application	158:168	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	5	54	theme	Composite	722:730	arg1	sponges					732:738	Composite sponges	722:738	Composite sponges	722:738	Composite sponges were prepared using lyophilization technique.
29715500	1	55	theme	lyophilized	212:222	arg1	sponges					224:230	lyophilized sponges	212:230	lyophilized sponges for transmucosal drug delivery	212:261	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	10	56	theme	HPMC	1399:1402	arg1	Incorporation					1382:1394	Incorporation	1382:1394	Incorporation of HPMC into CS-sponges	1382:1418	Incorporation of HPMC into CS-sponges significantly enhanced the mucoadhesion time to 6 h, while CS-sponges lost contact after around 10 min.
29715500	6	57	dep	strength	880:887	arg1	determination					889:901	determination	889:901	mechanical strength determination	869:901	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	6	58	theme	uptake	1001:1006	arg1	capacity					1008:1015	water uptake capacity	995:1015	water uptake capacity	995:1015	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	7	59	theme	selected	1093:1100	arg1	sponges					1102:1108	selected sponges	1093:1108	selected sponges	1093:1108	Furthermore, the surface topology of selected sponges was viewed using scanning electron microscope.
29715500	7	60	theme	surface	1073:1079	arg1	topology					1081:1088	the surface topology	1069:1088	the surface topology of selected sponges	1069:1108	Furthermore, the surface topology of selected sponges was viewed using scanning electron microscope.
29715500	11	61	theme	HPMC	1562:1565	arg1	sponges					1567:1573	HPMC sponges	1562:1573	HPMC sponges stored in low humidity conditions	1562:1607	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	0	62	theme	composite	78:86	arg1	sponges					88:94	buccal chitosan-based composite sponges	56:94	buccal chitosan-based composite sponges	56:94	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	9	63	theme	best	1311:1314	arg1	characteristics					1332:1346	the best physicochemical characteristics	1307:1346	the best physicochemical characteristics suitable for buccal applications	1307:1379	Among all fabricated sponges, sponges fabricated of CS:HPMC (1:1) showed the best physicochemical characteristics suitable for buccal applications.
29715500	1	64	theme	application	158:168	arg1	course					148:153	the course	144:153	the course of application and modernization of buccal dosage forms	144:209	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	4	65	theme	CS-based	694:701	arg1	sponges					713:719	buccal CS-based composite sponges	687:719	buccal CS-based composite sponges	687:719	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	11	66	theme	low	1585:1587	arg1	conditions					1598:1607	low humidity conditions	1585:1607	low humidity conditions	1585:1607	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	5	67	theme	lyophilization	760:773	arg1	technique					775:783	lyophilization technique	760:783	lyophilization technique	760:783	Composite sponges were prepared using lyophilization technique.
29715500	1	68	theme	modernization	174:186	arg1	course					148:153	the course	144:153	the course of application and modernization of buccal dosage forms	144:209	In the course of application and modernization of buccal dosage forms, lyophilized sponges for transmucosal drug delivery symbolize one of the most attractive approaches.
29715500	4	69	theme	sponges	713:719	arg1	properties					673:682	the mucoadhesive properties	656:682	the mucoadhesive properties of buccal CS-based composite sponges	656:719	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	0	70	theme	mucoadhesive	111:122	arg1	characteristics					124:138	augmented mucoadhesive characteristics	101:138	augmented mucoadhesive characteristics	101:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	6	71	theme	porosity	1034:1041	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	2	72	theme	forms	409:413	arg1	Chitosan					312:319	Chitosan	312:319	Chitosan (CS)	312:324	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	2	72	theme	forms	409:413	arg1	material					373:380	a forming material	363:380	a forming material of different buccal dosage forms including sponges	363:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	0	73	theme	polymer	11:17	arg1	approach					28:35	polymer blending approach	11:35	polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics	11:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	4	74	theme	first	574:578	arg1	time					580:583	the first time	570:583	the first time	570:583	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	2	75	theme	buccal	395:400	arg1	forms					409:413	different buccal dosage forms	385:413	different buccal dosage forms including sponges	385:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	2	75	theme	buccal	395:400	arg1	sponges					425:431	sponges	425:431	sponges	425:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	6	76	dep	transform	912:920	arg1	infrared					922:929	infrared	922:929	transform infrared spectroscopic analysis (FT-IR)	912:960	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	11	77	theme	Preliminary	1524:1534	arg1	study					1546:1550	Preliminary stability study	1524:1550	Preliminary stability study	1524:1550	Preliminary stability study showed CS:HPMC sponges stored in low humidity conditions maintain their soft texture and mucoadhesive properties for one month.
29715500	2	78	theme	different	385:393	arg1	forms					409:413	different buccal dosage forms	385:413	different buccal dosage forms including sponges	385:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	2	78	theme	different	385:393	arg1	sponges					425:431	sponges	425:431	sponges	425:431	Chitosan (CS) has been extensively investigated as a forming material of different buccal dosage forms including sponges.
29715500	0	79	theme	buccal	56:61	arg1	sponges					88:94	buccal chitosan-based composite sponges	56:94	buccal chitosan-based composite sponges	56:94	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29715500	6	80	theme	in-vitro	795:802	arg1	characterization					804:819	Thorough in-vitro characterization	786:819	Thorough in-vitro characterization of the proposed sponges	786:843	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	8	81	theme	sponges	1188:1194	arg1	properties					1174:1183	The mucoadhesive properties	1157:1183	The mucoadhesive properties of sponges	1157:1194	The mucoadhesive properties of sponges were tested both ex-vivo and in-vivo.
29715500	6	82	theme	Fourier	904:910	arg1	assessments					1043:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments	869:1053	Thorough in-vitro characterization of the proposed sponges was performed including mechanical strength determination, Fourier transform infrared spectroscopic analysis (FT-IR), surface pH, % moisture content, water uptake capacity, viscosity and % porosity assessments.
29715500	10	83	theme	mucoadhesion	1447:1458	arg1	time					1460:1463	the mucoadhesion time	1443:1463	the mucoadhesion time to 6 h	1443:1470	Incorporation of HPMC into CS-sponges significantly enhanced the mucoadhesion time to 6 h, while CS-sponges lost contact after around 10 min.
29715500	3	84	theme	weak	510:513	arg1	strength					524:531	weak adhesion strength	510:531	weak adhesion strength	510:531	However, CS-based buccal delivery systems suffer from many limitations like weak adhesion strength and poor tensile properties.
29715500	4	85	theme	blending	627:634	arg1	approach					636:643	the polymer blending approach	615:643	the polymer blending approach	615:643	So, for the first time, the current study focused on the polymer blending approach to enhance the mucoadhesive properties of buccal CS-based composite sponges.
29715500	0	86	with	fabrication	41:51	arg1	characteristics					124:138	augmented mucoadhesive characteristics	101:138	augmented mucoadhesive characteristics	101:138	Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics.
29366768	4	0	theme	oral	1006:1009	arg1	group					1036:1040	GP group	1033:1040	GP group	1033:1040	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	0	theme	oral	1006:1009	arg1	administration					1011:1024	daily oral administration	1000:1024	daily oral administration of GP (GP group)	1000:1041	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	3	1	theme	dextran	739:745	arg1	DSS					764:766	DSS	764:766	DSS	764:766	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	1	theme	dextran	739:745	arg1	sodium					756:761	a dextran sulphate sodium	737:761	a dextran sulphate sodium (DSS)	737:767	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	16	2	theme	gut	2755:2757	arg1	microbiota					2759:2768	gut microbiota	2755:2768	gut microbiota	2755:2768	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	2	3	theme	ginseng	337:343	arg1	GP					362:363	GP	362:363	GP	362:363	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	2	3	theme	ginseng	337:343	arg1	polysaccharides					345:359	ginseng polysaccharides	337:359	ginseng polysaccharides (GP)	337:364	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	11	4	theme	microbial	1943:1951	arg1	composition					1953:1963	the microbial composition	1939:1963	the microbial composition	1939:1963	GP influenced the microbial composition and showed a prebiotic-like effect.
29366768	15	5	theme	systemic	2583:2590	arg1	exposure					2592:2599	the systemic exposure	2579:2599	the systemic exposure of Rb1	2579:2606	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	11	6	theme	prebiotic-like	1978:1991	arg1	effect					1993:1998	a prebiotic-like effect	1976:1998	a prebiotic-like effect	1976:1998	GP influenced the microbial composition and showed a prebiotic-like effect.
29366768	6	7	theme	GP	1398:1399	arg1	presence/absence					1378:1393	presence/absence	1378:1393	presence/absence of GP	1378:1399	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	13	8	theme	quicker	2145:2151	arg1	metabolism					2163:2172	quicker microbial metabolism	2145:2172	quicker microbial metabolism of Rb1	2145:2179	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	4	9	theme	fecal	1058:1062	arg1	samples					1064:1070	fecal samples	1058:1070	fecal samples	1058:1070	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	16	10	theme	multifaceted	2777:2788	arg1	action					2790:2795	the multifaceted action	2773:2795	the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs	2773:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	4	11	theme	drinking	935:942	arg1	water					944:948	drinking water	935:948	drinking water (normal group)	935:963	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	11	theme	drinking	935:942	arg1	group					958:962	normal group	951:962	normal group	951:962	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	15	12	theme	Rb1	2692:2694	arg1	deglycosylation					2636:2650	microbial deglycosylation	2626:2650	microbial deglycosylation	2626:2650	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	15	12	theme	Rb1	2692:2694	arg1	absorption					2678:2687	intestinal epithelial absorption	2656:2687	intestinal epithelial absorption	2656:2687	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	10	13	theme	Rb1	1803:1805	arg1	AUC0-t					1784:1789	AUC0-t	1784:1789	AUC0-t	1784:1789	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	10	13	theme	Rb1	1803:1805	arg1	Cmax					1795:1798	Cmax	1795:1798	Cmax	1795:1798	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	0	14	theme	microbial	142:150	arg1	metabolism					152:161	gut microbial metabolism	138:161	gut microbial metabolism	138:161	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	16	15	from	role	2747:2750	arg1	action					2790:2795	the multifaceted action	2773:2795	the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs	2773:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	3	16	from	impact	606:611	arg1	pharmacokinetics					627:642	oral pharmacokinetics	622:642	oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	622:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	17	theme	colitis	790:796	arg1	model					798:802	experimental colitis model	777:802	experimental colitis model which was characterized by gut dysbiosis	777:843	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	15	18	theme	epithelial	2667:2676	arg1	absorption					2678:2687	intestinal epithelial absorption	2656:2687	intestinal epithelial absorption	2656:2687	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	3	19	theme	ginsenoside	647:657	arg1	ginsenoside					710:720	the dominant protopanoxadiol (PPD)-type ginsenoside	670:720	the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	670:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	19	theme	ginsenoside	647:657	arg1	Rb1					664:666	Rb1	664:666	Rb1	664:666	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	19	theme	ginsenoside	647:657	arg1	Rb1					659:661	ginsenoside Rb1	647:661	ginsenoside Rb1 (Rb1)	647:667	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	8	20	theme	fecal	1563:1567	arg1	samples					1569:1575	fecal samples	1563:1575	fecal samples	1563:1575	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	1	21	theme	Chinese	278:284	arg1	TCMs					297:300	TCMs	297:300	TCMs	297:300	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	1	21	theme	Chinese	278:284	arg1	medicines					286:294	traditional Chinese medicines	266:294	traditional Chinese medicines (TCMs)	266:301	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	6	22	dep	in	1223:1224	arg1	vitro					1226:1230	vitro	1226:1230	vitro	1226:1230	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	16	23	theme	TCMs	2868:2871	arg1	decoction					2855:2863	traditional decoction	2843:2863	traditional decoction of TCMs	2843:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	16	24	theme	important	2737:2745	arg1	role					2747:2750	the important role	2733:2750	the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs	2733:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	14	25	theme	Caco-2	2413:2418	arg1	monolayer					2420:2428	the Caco-2 monolayer	2409:2428	the Caco-2 monolayer	2409:2428	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	10	26	theme	elevated	1851:1858	arg1	AUC0-t					1860:1865	elevated AUC0-t	1851:1865	elevated AUC0-t of the metabolites	1851:1884	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	15	27	theme	microbial	2626:2634	arg1	deglycosylation					2636:2650	microbial deglycosylation	2626:2650	microbial deglycosylation	2626:2650	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	2	28	theme	previous	308:315	arg1	study					317:321	Our previous study	304:321	Our previous study	304:321	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	1	29	theme	ETHNOPHARMACOLOGICAL	164:183	arg1	Polysaccharides					195:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides	164:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides	164:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	4	30	theme	%	967:967	arg1	DSS					969:971	5% DSS	966:971	5% DSS (UC group)	966:982	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	30	theme	%	967:967	arg1	group					977:981	UC group	974:981	UC group	974:981	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	14	31	dep	cm/s	2487:2490	arg1	to					2492:2493	to	2492:2493	to	2492:2493	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	6	32	theme	gut	1259:1261	arg1	microbiota					1263:1272	gut microbiota	1259:1272	gut microbiota from normal and UC rats	1259:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	4	33	theme	%	989:989	arg1	DSS					991:993	5% DSS	988:993	5% DSS	988:993	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	8	34	theme	16S	1602:1604	arg1	RT-PCR					1617:1622	16S rRNA-based RT-PCR	1602:1622	16S rRNA-based RT-PCR	1602:1622	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	14	35	theme	Rb1	2388:2390	arg1	transport					2392:2400	Rb1 transport	2388:2400	Rb1 transport across the Caco-2 monolayer in both directions	2388:2447	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	12	36	theme	DSS	2099:2101	arg1	induction					2103:2111	DSS induction	2099:2111	DSS induction	2099:2111	Accordingly, GP treatment could partially restore the β-glucosidase activity which was reduced by DSS induction.
29366768	5	37	theme	oral	1140:1143	arg1	dosage					1145:1150	an oral dosage	1137:1150	an oral dosage of Rb1	1137:1157	On day 7 all animals received an oral dosage of Rb1 and blood samples were withdrawn for pharmacokinetic study.
29366768	10	38	theme	metabolites	1874:1884	arg1	AUC0-t					1860:1865	elevated AUC0-t	1851:1865	elevated AUC0-t of the metabolites	1851:1884	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	4	39	dep	MATERIALS	899:907	arg1	received					926:933	received	926:933	received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples	926:1070	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	1	40	theme	small	215:219	arg1	molecules					221:229	small molecules	215:229	small molecules	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	3	41	theme	gut	831:833	arg1	dysbiosis					835:843	gut dysbiosis	831:843	gut dysbiosis	831:843	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	14	42	theme	cell	2360:2363	arg1	growth					2365:2370	Caco-2 cell growth	2353:2370	Caco-2 cell growth	2353:2370	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	15	43	theme	colitis-like	2544:2555	arg1	symptoms					2557:2564	DSS-induced colitis-like symptoms	2532:2564	DSS-induced colitis-like symptoms	2532:2564	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	15	44	theme	CONCLUSIONS	2506:2516	arg1	GP					2518:2519	CONCLUSIONS GP	2506:2519	CONCLUSIONS GP	2506:2519	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	2	45	theme	involved	507:514	arg1	mechanisms					516:525	gut microbiota involved mechanisms	492:525	gut microbiota involved mechanisms	492:525	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	0	46	theme	Ginseng	0:6	arg1	polysaccharides					8:22	Ginseng polysaccharides	0:22	Ginseng polysaccharides	0:22	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	2	47	theme	gut	492:494	arg1	mechanisms					516:525	gut microbiota involved mechanisms	492:525	gut microbiota involved mechanisms	492:525	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	15	48	theme	DSS-induced	2532:2542	arg1	symptoms					2557:2564	DSS-induced colitis-like symptoms	2532:2564	DSS-induced colitis-like symptoms	2532:2564	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	14	49	theme	Caco-2	2353:2358	arg1	growth					2365:2370	Caco-2 cell growth	2353:2370	Caco-2 cell growth	2353:2370	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	13	50	from	formation	2195:2203	arg1	8 h					2214:2216	first 8 h	2208:2216	first 8 h of incubation	2208:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	7	51	theme	Compound	1478:1485	arg1	metabolites					1424:1434	its bacterial metabolites	1410:1434	its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD	1410:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	51	theme	Compound	1478:1485	arg1	CK					1490:1491	CK	1490:1491	CK	1490:1491	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	51	theme	Compound	1478:1485	arg1	K					1487:1487	Compound K	1478:1487	Compound K (CK)	1478:1492	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	5	52	theme	pharmacokinetic	1196:1210	arg1	study					1212:1216	pharmacokinetic study	1196:1216	pharmacokinetic study	1196:1216	On day 7 all animals received an oral dosage of Rb1 and blood samples were withdrawn for pharmacokinetic study.
29366768	2	53	theme	cold	470:473	arg1	model					482:486	an over-fatigue and acute cold stress model	444:486	an over-fatigue and acute cold stress model	444:486	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	16	54	theme	holistic	2823:2830	arg1	actions					2832:2838	the holistic actions	2819:2838	the holistic actions of traditional decoction of TCMs	2819:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	16	55	theme	decoction	2855:2863	arg1	actions					2832:2838	the holistic actions	2819:2838	the holistic actions of traditional decoction of TCMs	2819:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	0	56	theme	microbial	53:61	arg1	metabolites					63:73	microbial metabolites	53:73	microbial metabolites	53:73	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	0	57	dep	Rb1	45:47	arg1	exposure					75:82	exposure	75:82	exposure	75:82	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	4	58	theme	GP	1029:1030	arg1	water					944:948	drinking water	935:948	drinking water (normal group)	935:963	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	samples					1064:1070	fecal samples	1058:1070	fecal samples	1058:1070	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	group					1036:1040	GP group	1033:1040	GP group	1033:1040	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	administration					1011:1024	daily oral administration	1000:1024	daily oral administration of GP (GP group)	1000:1041	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	DSS					969:971	5% DSS	966:971	5% DSS (UC group)	966:982	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	group					958:962	normal group	951:962	normal group	951:962	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	DSS					991:993	5% DSS	988:993	5% DSS	988:993	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	58	theme	GP	1029:1030	arg1	group					977:981	UC group	974:981	UC group	974:981	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	3	59	theme	-type	704:708	arg1	ginsenoside					710:720	the dominant protopanoxadiol (PPD)-type ginsenoside	670:720	the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	670:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	59	theme	-type	704:708	arg1	Rb1					659:661	ginsenoside Rb1	647:661	ginsenoside Rb1 (Rb1)	647:667	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	8	60	from	bacteria	1551:1558	arg1	samples					1569:1575	fecal samples	1563:1575	fecal samples	1563:1575	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	1	61	theme	medicines	286:294	arg1	decoctions					252:261	decoctions	252:261	decoctions of traditional Chinese medicines (TCMs)	252:301	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	6	62	from	study	1316:1320	arg1	rats					1293:1296	normal and UC rats	1279:1296	normal and UC rats	1279:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	62	from	study	1316:1320	arg1	microbiota					1263:1272	gut microbiota	1259:1272	gut microbiota from normal and UC rats	1259:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	14	63	theme	10-7	2482:2485	arg1	cm/s					2487:2490	cm/s	2487:2490	cm/s	2487:2490	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	4	64	theme	GP	1033:1034	arg1	group					1036:1040	GP group	1033:1040	GP group	1033:1040	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	64	theme	GP	1033:1034	arg1	administration					1011:1024	daily oral administration	1000:1024	daily oral administration of GP (GP group)	1000:1041	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	14	65	from	transport	2392:2400	arg1	directions					2438:2447	both directions	2433:2447	both directions	2433:2447	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	13	66	from	metabolism	2163:2172	arg1	8 h					2214:2216	first 8 h	2208:2216	first 8 h of incubation	2208:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	6	67	theme	Caco-2	1336:1341	arg1	monolayer					1348:1356	Caco-2 cell monolayer	1336:1356	Caco-2 cell monolayer	1336:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	16	68	theme	traditional	2843:2853	arg1	decoction					2855:2863	traditional decoction	2843:2863	traditional decoction of TCMs	2843:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	13	69	theme	higher	2185:2190	arg1	formation					2195:2203	higher Rd formation	2185:2203	higher Rd formation in first 8 h of incubation	2185:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	9	70	theme	4-nitrophenyl-β-D-glucopyranoside	1702:1734	arg1	hydrolysis					1736:1745	4-nitrophenyl-β-D-glucopyranoside hydrolysis	1702:1745	4-nitrophenyl-β-D-glucopyranoside hydrolysis	1702:1745	β-Glucosidase activity was determined by measuring 4-nitrophenol formed from 4-nitrophenyl-β-D-glucopyranoside hydrolysis.
29366768	15	71	theme	Rb1	2604:2606	arg1	exposure					2592:2599	the systemic exposure	2579:2599	the systemic exposure of Rb1	2579:2606	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	6	72	theme	Rb1	1325:1327	arg1	study					1243:1247	The in vitro metabolism study	1219:1247	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats	1219:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	72	theme	Rb1	1325:1327	arg1	study					1316:1320	the transport study	1302:1320	the transport study of Rb1 across Caco-2 cell monolayer	1302:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	10	73	theme	DSS	1756:1758	arg1	induction					1760:1768	DSS induction	1756:1768	DSS induction	1756:1768	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	16	74	theme	microbiota	2759:2768	arg1	role					2747:2750	the important role	2733:2750	the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs	2733:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	10	75	theme	GP	1915:1916	arg1	group					1918:1922	GP group	1915:1922	GP group	1915:1922	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	3	76	theme	sulphate	747:754	arg1	DSS					764:766	DSS	764:766	DSS	764:766	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	76	theme	sulphate	747:754	arg1	sodium					756:761	a dextran sulphate sodium	737:761	a dextran sulphate sodium (DSS)	737:767	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	6	77	theme	Rb1	1252:1254	arg1	study					1243:1247	The in vitro metabolism study	1219:1247	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats	1219:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	77	theme	Rb1	1252:1254	arg1	study					1316:1320	the transport study	1302:1320	the transport study of Rb1 across Caco-2 cell monolayer	1302:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	3	78	theme	GP	616:617	arg1	impact					606:611	the impact	602:611	the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	602:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	13	79	theme	microbial	2153:2161	arg1	metabolism					2163:2172	quicker microbial metabolism	2145:2172	quicker microbial metabolism of Rb1	2145:2179	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	6	80	theme	in	1223:1224	arg1	study					1243:1247	The in vitro metabolism study	1219:1247	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats	1219:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	13	81	theme	first	2208:2212	arg1	8 h					2214:2216	first 8 h	2208:2216	first 8 h of incubation	2208:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	3	82	from	ginsenoside	710:720	arg1	Ginseng					725:731	Ginseng	725:731	Ginseng	725:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	15	83	theme	intestinal	2656:2665	arg1	absorption					2678:2687	intestinal epithelial absorption	2656:2687	intestinal epithelial absorption	2656:2687	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	6	84	from	rats	1293:1296	arg1	study					1243:1247	The in vitro metabolism study	1219:1247	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats	1219:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	84	from	rats	1293:1296	arg1	study					1316:1320	the transport study	1302:1320	the transport study of Rb1 across Caco-2 cell monolayer	1302:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	84	from	rats	1293:1296	arg1	microbiota					1263:1272	gut microbiota	1259:1272	gut microbiota from normal and UC rats	1259:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	8	85	theme	target	1544:1549	arg1	bacteria					1551:1558	target bacteria	1544:1558	target bacteria	1544:1558	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	1	86	theme	traditional	266:276	arg1	TCMs					297:300	TCMs	297:300	TCMs	297:300	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	1	86	theme	traditional	266:276	arg1	medicines					286:294	traditional Chinese medicines	266:294	traditional Chinese medicines (TCMs)	266:301	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	7	87	theme	ginsenoside	1457:1467	arg1	F2					1473:1474	F2	1473:1474	F2	1473:1474	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	87	theme	ginsenoside	1457:1467	arg1	metabolites					1424:1434	its bacterial metabolites	1410:1434	its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD	1410:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	87	theme	ginsenoside	1457:1467	arg1	F2					1469:1470	ginsenoside F2	1457:1470	ginsenoside F2 (F2)	1457:1475	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	3	88	theme	oral	622:625	arg1	pharmacokinetics					627:642	oral pharmacokinetics	622:642	oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	622:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	15	89	gly	deglycosylation	2636:2650	arg1	Rb1					2692:2694	Rb1	2692:2694	Rb1	2692:2694	CONCLUSIONS GP alleviated DSS-induced colitis-like symptoms and enhanced the systemic exposure of Rb1 through enhancing microbial deglycosylation and intestinal epithelial absorption of Rb1.
29366768	5	90	theme	Rb1	1155:1157	arg1	dosage					1145:1150	an oral dosage	1137:1150	an oral dosage of Rb1	1137:1157	On day 7 all animals received an oral dosage of Rb1 and blood samples were withdrawn for pharmacokinetic study.
29366768	3	91	theme	experimental	777:788	arg1	model					798:802	experimental colitis model	777:802	experimental colitis model which was characterized by gut dysbiosis	777:843	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	13	92	theme	CK	2260:2261	arg1	formation/conversion					2263:2282	CK formation/conversion	2260:2282	CK formation/conversion	2260:2282	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	3	93	theme	underlying	867:876	arg1	mechanisms					878:887	the underlying mechanisms	863:887	the underlying mechanisms	863:887	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	5	94	theme	blood	1163:1167	arg1	samples					1169:1175	blood samples	1163:1175	blood samples	1163:1175	On day 7 all animals received an oral dosage of Rb1 and blood samples were withdrawn for pharmacokinetic study.
29366768	7	95	dep	metabolites	1424:1434	arg1	F2					1469:1470	ginsenoside F2	1457:1470	ginsenoside F2 (F2)	1457:1475	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	K					1487:1487	Compound K	1478:1487	Compound K (CK)	1478:1492	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	Rd					1448:1449	ginsenoside Rd	1436:1449	ginsenoside Rd (Rd)	1436:1454	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	CK					1490:1491	CK	1490:1491	CK	1490:1491	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	Rd					1452:1453	Rd	1452:1453	Rd	1452:1453	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	F2					1473:1474	F2	1473:1474	F2	1473:1474	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	metabolites					1424:1434	its bacterial metabolites	1410:1434	its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD	1410:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	95	dep	metabolites	1424:1434	arg1	PPD					1498:1500	PPD	1498:1500	PPD	1498:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	3	96	theme	Rb1	659:661	arg1	pharmacokinetics					627:642	oral pharmacokinetics	622:642	oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	622:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	1	97	theme	RELEVANCE	185:193	arg1	Polysaccharides					195:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides	164:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides	164:209	ETHNOPHARMACOLOGICAL RELEVANCE Polysaccharides and small molecules commonly co-exist in decoctions of traditional Chinese medicines (TCMs).
29366768	7	98	theme	ginsenoside	1436:1446	arg1	Rd					1448:1449	ginsenoside Rd	1436:1449	ginsenoside Rd (Rd)	1436:1454	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	98	theme	ginsenoside	1436:1446	arg1	metabolites					1424:1434	its bacterial metabolites	1410:1434	its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD	1410:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	98	theme	ginsenoside	1436:1446	arg1	Rd					1452:1453	Rd	1452:1453	Rd	1452:1453	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	13	99	from	impact	2243:2248	arg1	F2					2253:2254	F2	2253:2254	F2	2253:2254	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	13	99	from	impact	2243:2248	arg1	formation/conversion					2263:2282	CK formation/conversion	2260:2282	CK formation/conversion	2260:2282	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	0	100	theme	intestinal	102:111	arg1	absorption					113:122	intestinal absorption	102:122	intestinal absorption	102:122	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	7	101	theme	bacterial	1414:1422	arg1	Rd					1448:1449	ginsenoside Rd	1436:1449	ginsenoside Rd (Rd)	1436:1454	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	101	theme	bacterial	1414:1422	arg1	F2					1469:1470	ginsenoside F2	1457:1470	ginsenoside F2 (F2)	1457:1475	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	101	theme	bacterial	1414:1422	arg1	metabolites					1424:1434	its bacterial metabolites	1410:1434	its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD	1410:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	101	theme	bacterial	1414:1422	arg1	PPD					1498:1500	PPD	1498:1500	PPD	1498:1500	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	7	101	theme	bacterial	1414:1422	arg1	K					1487:1487	Compound K	1478:1487	Compound K (CK)	1478:1492	Rb1 and its bacterial metabolites ginsenoside Rd (Rd), ginsenoside F2 (F2), Compound K (CK) and PPD were determined using LC-MS/MS.
29366768	13	102	theme	Rb1	2177:2179	arg1	formation					2195:2203	higher Rd formation	2185:2203	higher Rd formation in first 8 h of incubation	2185:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	13	102	theme	Rb1	2177:2179	arg1	metabolism					2163:2172	quicker microbial metabolism	2145:2172	quicker microbial metabolism of Rb1	2145:2179	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	8	103	theme	rRNA-based	1606:1615	arg1	RT-PCR					1617:1622	16S rRNA-based RT-PCR	1602:1622	16S rRNA-based RT-PCR	1602:1622	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	4	104	theme	normal	951:956	arg1	water					944:948	drinking water	935:948	drinking water (normal group)	935:963	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	104	theme	normal	951:956	arg1	group					958:962	normal group	951:962	normal group	951:962	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	6	105	theme	metabolism	1232:1241	arg1	study					1243:1247	The in vitro metabolism study	1219:1247	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats	1219:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	12	106	theme	β-glucosidase	2055:2067	arg1	activity					2069:2076	the β-glucosidase activity	2051:2076	the β-glucosidase activity which was reduced by DSS induction	2051:2111	Accordingly, GP treatment could partially restore the β-glucosidase activity which was reduced by DSS induction.
29366768	0	107	theme	gut	138:140	arg1	metabolism					152:161	gut microbial metabolism	138:161	gut microbial metabolism	138:161	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	6	108	theme	transport	1306:1314	arg1	study					1316:1320	the transport study	1302:1320	the transport study of Rb1 across Caco-2 cell monolayer	1302:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	109	from	study	1243:1247	arg1	rats					1293:1296	normal and UC rats	1279:1296	normal and UC rats	1279:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	6	109	from	study	1243:1247	arg1	microbiota					1263:1272	gut microbiota	1259:1272	gut microbiota from normal and UC rats	1259:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	2	110	theme	microbiota	496:505	arg1	mechanisms					516:525	gut microbiota involved mechanisms	492:525	gut microbiota involved mechanisms	492:525	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	12	111	theme	GP	2014:2015	arg1	treatment					2017:2025	GP treatment	2014:2025	GP treatment	2014:2025	Accordingly, GP treatment could partially restore the β-glucosidase activity which was reduced by DSS induction.
29366768	0	112	theme	ginsenoside	33:43	arg1	Rb1					45:47	ginsenoside Rb1	33:47	ginsenoside Rb1	33:47	Ginseng polysaccharides enhanced ginsenoside Rb1 and microbial metabolites exposure through enhancing intestinal absorption and affecting gut microbial metabolism.
29366768	13	113	theme	incubation	2221:2230	arg1	8 h					2214:2216	first 8 h	2208:2216	first 8 h of incubation	2208:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	9	114	theme	β-Glucosidase	1625:1637	arg1	activity					1639:1646	β-Glucosidase activity	1625:1646	β-Glucosidase activity	1625:1646	β-Glucosidase activity was determined by measuring 4-nitrophenol formed from 4-nitrophenyl-β-D-glucopyranoside hydrolysis.
29366768	3	115	theme	dominant	674:681	arg1	ginsenoside					710:720	the dominant protopanoxadiol (PPD)-type ginsenoside	670:720	the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng	670:731	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	3	115	theme	dominant	674:681	arg1	Rb1					659:661	ginsenoside Rb1	647:661	ginsenoside Rb1 (Rb1)	647:667	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	2	116	theme	stress	475:480	arg1	model					482:486	an over-fatigue and acute cold stress model	444:486	an over-fatigue and acute cold stress model	444:486	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	6	117	theme	UC	1290:1291	arg1	rats					1293:1296	normal and UC rats	1279:1296	normal and UC rats	1279:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	13	118	theme	GP	2130:2131	arg1	presence					2118:2125	The presence	2114:2125	The presence of GP	2114:2131	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	13	119	theme	Rd	2192:2193	arg1	formation					2195:2203	higher Rd formation	2185:2203	higher Rd formation in first 8 h of incubation	2185:2230	The presence of GP resulted in quicker microbial metabolism of Rb1 and higher Rd formation in first 8 h of incubation, while the impact on F2 and CK formation/conversion became obvious after 8 h.
29366768	16	120	theme	polysaccharides	2800:2814	arg1	action					2790:2795	the multifaceted action	2773:2795	the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs	2773:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	2	121	theme	acute	464:468	arg1	model					482:486	an over-fatigue and acute cold stress model	444:486	an over-fatigue and acute cold stress model	444:486	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	6	122	theme	normal	1279:1284	arg1	rats					1293:1296	normal and UC rats	1279:1296	normal and UC rats	1279:1296	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	16	123	from	action	2790:2795	arg1	actions					2832:2838	the holistic actions	2819:2838	the holistic actions of traditional decoction of TCMs	2819:2871	These findings further demonstrated the important role of gut microbiota in the multifaceted action of polysaccharides in the holistic actions of traditional decoction of TCMs.
29366768	14	124	theme	10-6	2495:2498	arg1	cm/s					2487:2490	cm/s	2487:2490	cm/s	2487:2490	More interestingly, GP slightly stimulated Caco-2 cell growth and facilitated Rb1 transport across the Caco-2 monolayer in both directions, increasing the Papp of Rb1 from 10-7 cm/s to 10-6 cm/s.
29366768	2	125	theme	co-existing	386:396	arg1	ginsenosides					398:409	co-existing ginsenosides	386:409	co-existing ginsenosides	386:409	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	10	126	theme	particular	1890:1899	arg1	Rd					1901:1902	particular Rd	1890:1902	particular Rd	1890:1902	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	3	127	theme	STUDY	539:543	arg1	AIM					528:530	AIM	528:530	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS)	528:767	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	6	128	theme	cell	1343:1346	arg1	monolayer					1348:1356	Caco-2 cell monolayer	1336:1356	Caco-2 cell monolayer	1336:1356	The in vitro metabolism study of Rb1 in gut microbiota from normal and UC rats and the transport study of Rb1 across Caco-2 cell monolayer were carried out in presence/absence of GP.
29366768	3	129	dep	AIM	528:530	arg1	aimed					556:560	aimed	556:560	aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS)	556:767	AIM OF THE STUDY This study aimed to verify the interactions by examining the impact of GP on oral pharmacokinetics of ginsenoside Rb1 (Rb1), the dominant protopanoxadiol (PPD)-type ginsenoside in Ginseng, on a dextran sulphate sodium (DSS) induced experimental colitis model which was characterized by gut dysbiosis, and to delineate the underlying mechanisms in vitro.
29366768	4	130	theme	daily	1000:1004	arg1	group					1036:1040	GP group	1033:1040	GP group	1033:1040	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	4	130	theme	daily	1000:1004	arg1	administration					1011:1024	daily oral administration	1000:1024	daily oral administration of GP (GP group)	1000:1041	MATERIALS AND METHODS Rats received drinking water (normal group), 5% DSS (UC group), or 5% DSS plus daily oral administration of GP (GP group) for 7 days and fecal samples were collected on day -3, 0 and 6.
29366768	2	131	theme	over-fatigue	447:458	arg1	model					482:486	an over-fatigue and acute cold stress model	444:486	an over-fatigue and acute cold stress model	444:486	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
29366768	10	132	dep	RESULTS	1748:1754	arg1	alter					1778:1782	alter	1778:1782	did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group	1770:1922	RESULTS DSS induction did not alter AUC0-t and Cmax of Rb1, which, however, were doubled together with elevated AUC0-t of the metabolites, in particular Rd and CK, in GP group.
29366768	8	133	from	Total	1534:1538	arg1	samples					1569:1575	fecal samples	1563:1575	fecal samples	1563:1575	Total and target bacteria in fecal samples were determined by using 16S rRNA-based RT-PCR.
29366768	2	134	theme	synergistic	422:432	arg1	effect					434:439	synergistic effect	422:439	synergistic effect	422:439	Our previous study outlined that ginseng polysaccharides (GP) could interact with co-existing ginsenosides to produce synergistic effect in an over-fatigue and acute cold stress model via gut microbiota involved mechanisms.
32123597	0	0	theme	monoclonal	81:90	arg1	antibodies					92:101	monoclonal antibodies	81:101	monoclonal antibodies	81:101	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	4	1	theme	only	862:865	arg1	method					867:872	the only method	858:872	the only method for quantitative analysis of glycoform heterogeneity	858:925	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	4	1	theme	only	862:865	arg1	it					848:849	it	848:849	it	848:849	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	1	2	theme	increasing	164:173	arg1	market					143:148	the biopharmaceutical market	121:148	the biopharmaceutical market exponentially increasing	121:173	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	3	theme	deeper	333:338	arg1	understanding					340:352	the deeper understanding	329:352	the deeper understanding of structure/function relationships	329:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	6	4	theme	data	1360:1363	arg1	application					1323:1333	application	1323:1333	application	1323:1333	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	6	4	theme	data	1360:1363	arg1	depth					1351:1355	the desired depth	1339:1355	the desired depth of data generated	1339:1373	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	3	5	theme	fast	679:682	arg1	methods					684:690	robust, accurate and fast methods	658:690	robust, accurate and fast methods	658:690	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	3	6	theme	methods	684:690	arg1	development					643:653	the development	639:653	the development of robust, accurate and fast methods to support drug development and quality control	639:738	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	6	7	theme	workflow	1301:1308	arg1	choice					1259:1264	the choice	1255:1264	the choice of the most appropriate analytical workflow	1255:1308	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	5	8	theme	analytical	957:966	arg1	workflows					968:976	ten different analytical workflows	943:976	ten different analytical workflows for N-glycan analysis	943:998	In this study, ten different analytical workflows for N-glycan analysis were compared using four monoclonal antibodies.
32123597	5	9	theme	different	947:955	arg1	workflows					968:976	ten different analytical workflows	943:976	ten different analytical workflows for N-glycan analysis	943:998	In this study, ten different analytical workflows for N-glycan analysis were compared using four monoclonal antibodies.
32123597	0	10	from	characterisation	48:63	arg1	antibodies					92:101	monoclonal antibodies	81:101	monoclonal antibodies	81:101	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	6	11	theme	analytical	1290:1299	arg1	workflow					1301:1308	the most appropriate analytical workflow	1269:1308	the most appropriate analytical workflow	1269:1308	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	2	12	attach	present	459:465	arg1	region					483:488	the constant region	470:488	the constant region of the heavy chains	470:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	12	attach	present	459:465	arg2	N-glycans					449:457	N-glycans	449:457	N-glycans present in the constant region of the heavy chains	449:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	13	from	present	459:465	arg1	region					483:488	the constant region	470:488	the constant region of the heavy chains	470:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	6	14	theme	appropriate	1278:1288	arg1	workflow					1301:1308	the most appropriate analytical workflow	1269:1308	the most appropriate analytical workflow	1269:1308	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	6	15	theme	good	1064:1067	arg1	comparability					1069:1081	good comparability	1064:1081	good comparability between the quantitative results generated	1064:1124	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	6	16	theme	technique	1198:1206	arg1	disadvantages					1176:1188	disadvantages	1176:1188	disadvantages	1176:1188	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	6	16	theme	technique	1198:1206	arg1	advantages					1161:1170	advantages	1161:1170	advantages	1161:1170	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	2	17	theme	monoclonal	394:403	arg1	antibodies					405:414	monoclonal antibodies	394:414	monoclonal antibodies	394:414	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	18	theme	chains	503:508	arg1	region					483:488	the constant region	470:488	the constant region of the heavy chains	470:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	3	19	theme	drug	703:706	arg1	development					708:718	drug development	703:718	drug development	703:718	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	5	20	theme	N-glycan	982:989	arg1	analysis					991:998	N-glycan analysis	982:998	N-glycan analysis	982:998	In this study, ten different analytical workflows for N-glycan analysis were compared using four monoclonal antibodies.
32123597	6	21	theme	quantitative	1095:1106	arg1	results					1108:1114	the quantitative results	1091:1114	the quantitative results generated	1091:1124	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	0	22	theme	different	10:18	arg1	domains					20:26	different domains	10:26	different domains of analysis	10:38	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	0	22	theme	different	10:18	arg1	analysis					31:38	analysis	31:38	analysis	31:38	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	1	23	theme	structure/function	357:374	arg1	relationships					376:388	structure/function relationships	357:388	structure/function relationships	357:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	2	24	theme	heavy	497:501	arg1	chains					503:508	the heavy chains	493:508	the heavy chains	493:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	3	25	theme	quality	724:730	arg1	control					732:738	quality control	724:738	quality control	724:738	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	1	26	theme	drug	263:266	arg1	safety					276:281	drug product safety	263:281	drug product safety	263:281	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	27	theme	aligned	188:194	arg1	growth					196:201	an aligned growth	185:201	an aligned growth in the importance of data-rich analyses	185:241	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	28	theme	product	268:274	arg1	safety					276:281	drug product safety	263:281	drug product safety	263:281	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	4	29	theme	N-glycan	750:757	arg1	standard					794:801	standard	794:801	standard	794:801	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	4	29	theme	N-glycan	750:757	arg1	analysis					759:766	Released N-glycan analysis	741:766	Released N-glycan analysis	741:766	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	4	30	theme	heterogeneity	913:925	arg1	analysis					891:898	quantitative analysis	878:898	quantitative analysis of glycoform heterogeneity	878:925	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	1	31	theme	relationships	376:388	arg1	understanding					340:352	the deeper understanding	329:352	the deeper understanding of structure/function relationships	329:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	4	32	theme	Released	741:748	arg1	standard					794:801	standard	794:801	standard	794:801	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	4	32	theme	Released	741:748	arg1	analysis					759:766	Released N-glycan analysis	741:766	Released N-glycan analysis	741:766	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	2	33	theme	many	417:420	arg1	functions					422:430	many functions	417:430	many functions	417:430	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	34	theme	constant	474:481	arg1	region					483:488	the constant region	470:488	the constant region of the heavy chains	470:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	35	theme	action	534:539	arg1	mechanisms					520:529	their mechanisms	514:529	their mechanisms of action	514:539	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	2	36	from	region	483:488	arg1	present					459:465	present	459:465	present	459:465	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	0	37	theme	analysis	31:38	arg1	domains					20:26	different domains	10:26	different domains of analysis	10:38	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	0	37	theme	analysis	31:38	arg1	analysis					31:38	analysis	31:38	analysis	31:38	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	3	38	theme	function	591:598	arg1	importance					571:580	The importance	567:580	The importance of their function	567:598	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	6	39	theme	desired	1343:1349	arg1	depth					1351:1355	the desired depth	1339:1355	the desired depth of data generated	1339:1373	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	2	40	theme	present	459:465	arg1	N-glycans					449:457	N-glycans	449:457	N-glycans present in the constant region of the heavy chains	449:508	In monoclonal antibodies, many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely known.
32123597	4	41	theme	quantitative	878:889	arg1	analysis					891:898	quantitative analysis	878:898	quantitative analysis of glycoform heterogeneity	878:925	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	3	42	theme	accurate	666:673	arg1	methods					684:690	robust, accurate and fast methods	658:690	robust, accurate and fast methods	658:690	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	1	43	dep	is	182:183	arg1	assist					295:300	assist	295:300	not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships	244:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	43	dep	is	182:183	arg1	assess					256:261	assess	256:261	not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships	244:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	44	from	growth	196:201	arg1	importance					210:219	the importance	206:219	the importance of data-rich analyses	206:241	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	45	theme	drug	302:305	arg1	development					307:317	drug development	302:317	drug development driven by the deeper understanding of structure/function relationships	302:388	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	4	46	theme	glycoform	903:911	arg1	heterogeneity					913:925	glycoform heterogeneity	903:925	glycoform heterogeneity	903:925	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	5	47	theme	monoclonal	1025:1034	arg1	antibodies					1036:1045	four monoclonal antibodies	1020:1045	four monoclonal antibodies	1020:1045	In this study, ten different analytical workflows for N-glycan analysis were compared using four monoclonal antibodies.
32123597	3	48	theme	research	619:626	arg1	efforts					628:634	analytical research efforts	608:634	analytical research efforts	608:634	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	4	49	theme	glycosylation	807:819	arg1	characterisation					821:836	glycosylation characterisation	807:836	glycosylation characterisation	807:836	Released N-glycan analysis is considered as the gold standard for glycosylation characterisation; however, it is not the only method for quantitative analysis of glycoform heterogeneity.
32123597	3	50	theme	robust	658:663	arg1	methods					684:690	robust, accurate and fast methods	658:690	robust, accurate and fast methods	658:690	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
32123597	6	51	dep	possible	1134:1141	arg1	summarise					1215:1223	summarise	1215:1223	to summarise all the observations	1212:1244	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	6	51	dep	possible	1134:1141	arg1	appreciate					1146:1155	appreciate	1146:1155	to appreciate the advantages and disadvantages of each technique	1143:1206	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	1	52	theme	biopharmaceutical	125:141	arg1	market					143:148	the biopharmaceutical market	121:148	the biopharmaceutical market exponentially increasing	121:173	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	1	53	theme	data-rich	224:232	arg1	analyses					234:241	data-rich analyses	224:241	data-rich analyses	224:241	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	0	54	theme	N-glycans	68:76	arg1	characterisation					48:63	the characterisation	44:63	the characterisation of N-glycans on monoclonal antibodies	44:101	Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies.
32123597	1	55	theme	market	143:148	arg1	size					113:116	the size	109:116	the size of the biopharmaceutical market exponentially increasing	109:173	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	6	56	dep	advantages	1161:1170	arg1	the					1157:1159	the	1157:1159	the	1157:1159	While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.
32123597	1	57	theme	analyses	234:241	arg1	importance					210:219	the importance	206:219	the importance of data-rich analyses	206:241	With the size of the biopharmaceutical market exponentially increasing, there is an aligned growth in the importance of data-rich analyses, not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function relationships.
32123597	3	58	theme	analytical	608:617	arg1	efforts					628:634	analytical research efforts	608:634	analytical research efforts	608:634	The importance of their function focuses analytical research efforts on the development of robust, accurate and fast methods to support drug development and quality control.
31532690	2	0	theme	nm	660:661	arg1	diameters					633:641	volumetric mean diameters	617:641	volumetric mean diameters of 100 nm vs 700 nm	617:661	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	11	1	theme	animal	2409:2414	arg1	models					2416:2421	larger animal models	2402:2421	larger animal models	2402:2421	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	1	2	theme	severe	285:290	arg1	effects					320:326	severe localized and systemic side effects	285:326	severe localized and systemic side effects	285:326	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	2	3	dep	sizes	610:614	arg1	diameters					633:641	volumetric mean diameters	617:641	volumetric mean diameters of 100 nm vs 700 nm	617:661	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	3	4	theme	murine	771:776	arg1	model					809:813	a murine subcutaneous colorectal cancer model	769:813	a murine subcutaneous colorectal cancer model	769:813	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	10	5	theme	animals	2205:2211	arg1	%					2196:2196	80%	2194:2196	80% of the animals in LPS-NE (small)	2194:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	10	5	theme	animals	2205:2211	arg1	animals					2205:2211	the animals	2201:2211	the animals in LPS-NE (small)	2201:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	5	6	theme	remission	1376:1384	arg1	case					1363:1366	the case	1359:1366	the case of full remission	1359:1384	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	11	7	theme	cargos	2526:2531	arg1	carriers					2479:2486	the carriers	2475:2486	the carriers of the incorporated immunotherapeutic cargos	2475:2531	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	6	8	theme	tolerability	1484:1495	arg1	determinant					1458:1468	an important determinant	1445:1468	an important determinant of short-term tolerability	1445:1495	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	6	8	theme	tolerability	1484:1495	arg1	Size					1436:1439	Size	1436:1439	Size	1436:1439	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	1	9	theme	pro-inflammatory	212:227	arg1	properties					229:238	the strong pro-inflammatory properties	201:238	the strong pro-inflammatory properties of lipopolysaccharide (LPS)	201:266	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	9	10	from	days	2049:2052	arg1	fact					1932:1935	fact	1932:1935	fact	1932:1935	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	10	11	theme	treatment	2157:2165	arg1	groups					2167:2172	other treatment groups	2151:2172	other treatment groups	2151:2172	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	2	12	theme	volumetric	617:626	arg1	diameters					633:641	volumetric mean diameters	617:641	volumetric mean diameters of 100 nm vs 700 nm	617:661	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	3	13	theme	colorectal	791:800	arg1	model					809:813	a murine subcutaneous colorectal cancer model	769:813	a murine subcutaneous colorectal cancer model	769:813	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	5	14	theme	animal	1123:1128	arg1	trials					1130:1135	the animal trials	1119:1135	the animal trials	1119:1135	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	11	15	dep	scale	2365:2369	arg1	larger					2358:2363	larger	2358:2363	larger	2358:2363	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	11	15	dep	scale	2365:2369	arg1	mechanistic-oriented					2372:2391	mechanistic-oriented	2372:2391	mechanistic-oriented	2372:2391	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	5	16	dep	%	1238:1238	arg1	15					1236:1237	15	1236:1237	15	1236:1237	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	9	17	theme	polymeric	2104:2112	arg1	groups					2136:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	5	18	theme	coat	1217:1220	arg1	BCS					1193:1195	BCS	1193:1195	BCS	1193:1195	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	5	18	theme	coat	1217:1220	arg1	score					1186:1190	a body condition score	1169:1190	a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight	1169:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	10	19	dep	LPS-NE	2216:2221	arg1	small					2224:2228	small	2224:2228	small	2224:2228	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	1	20	theme	side	315:318	arg1	effects					320:326	severe localized and systemic side effects	285:326	severe localized and systemic side effects	285:326	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	11	21	theme	better	2308:2313	arg1	understanding					2315:2327	a better understanding	2306:2327	a better understanding of these findings	2306:2345	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	5	22	theme	end	1106:1108	arg1	point					1110:1114	The toxicity-related end point	1085:1114	The toxicity-related end point of the animal trials	1085:1135	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	7	23	theme	systemic	1705:1712	arg1	tolerability					1714:1725	the long-term systemic tolerability	1691:1725	the long-term systemic tolerability	1691:1725	Nanostructure composition, on the other hand, predominantly governed the long-term systemic tolerability.
31532690	12	24	theme	selection	2575:2583	arg1	tuning					2630:2635	a purposeful tuning	2617:2635	a purposeful tuning of their physicochemical properties	2617:2671	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	12	24	theme	selection	2575:2583	arg1	importance					2554:2563	the importance	2550:2563	the importance of a wise selection of nanoparticle composition	2550:2611	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	5	25	theme	weight	1266:1271	arg1	loss					1240:1243	more than 15% loss	1226:1243	more than 15% loss of the original body weight	1226:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	5	25	theme	weight	1266:1271	arg1	coat					1217:1220	1 and poor hair coat	1201:1220	coat	1217:1220	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	9	26	from	groups	2136:2141	arg1	fact					1932:1935	fact	1932:1935	fact	1932:1935	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	2	27	with	nanostructures	517:530	arg1	compositions					547:558	different compositions	537:558	different compositions (lipid nanoemulsion vs polymeric nanospheres)	537:604	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	2	27	with	nanostructures	517:530	arg1	sizes					610:614	sizes	610:614	sizes (volumetric mean diameters of 100 nm vs 700 nm)	610:662	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	1	28	theme	holding	159:165	arg1	promise					167:173	holding promise	159:173	holding promise for cancer immunotherapy	159:198	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	6	29	dep	higher	1542:1547	arg1	extent					1563:1568	extent	1563:1568	extent	1563:1568	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	6	29	dep	higher	1542:1547	arg1	incidence					1549:1557	incidence	1549:1557	incidence	1549:1557	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	9	30	theme	mean	1942:1945	arg1	days					2049:2052	at least 42 days	2037:2052	at least 42 days longer than that of the LPS	2037:2080	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	9	30	theme	mean	1942:1945	arg1	survival					1947:1954	the mean survival	1938:1954	the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small))	1938:2031	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	9	31	dep	LPS-NE	2017:2022	arg1	small					2025:2029	small	2025:2029	small	2025:2029	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	10	32	theme	complete	2254:2261	arg1	remission					2263:2271	complete remission	2254:2271	complete remission	2254:2271	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	11	33	theme	scale	2365:2369	arg1	trial					2393:2397	a larger scale, mechanistic-oriented trial	2356:2397	a larger scale, mechanistic-oriented trial on larger animal models	2356:2421	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	12	34	theme	wise	2570:2573	arg1	selection					2575:2583	a wise selection	2568:2583	a wise selection of nanoparticle composition	2568:2611	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	5	35	theme	body	1171:1174	arg1	BCS					1193:1195	BCS	1193:1195	BCS	1193:1195	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	5	35	theme	body	1171:1174	arg1	score					1186:1190	a body condition score	1169:1190	a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight	1169:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	8	36	theme	LPS	1772:1774	arg1	molecules					1776:1784	LPS molecules	1772:1784	LPS molecules	1772:1784	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	12	37	theme	properties	2662:2671	arg1	tuning					2630:2635	a purposeful tuning	2617:2635	a purposeful tuning of their physicochemical properties	2617:2671	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	12	37	theme	properties	2662:2671	arg1	importance					2554:2563	the importance	2550:2563	the importance of a wise selection of nanoparticle composition	2550:2611	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	0	38	from	Modulation	0:9	arg1	Cancer					77:82	Cancer	77:82	Cancer	77:82	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	11	39	theme	findings	2338:2345	arg1	understanding					2315:2327	a better understanding	2306:2327	a better understanding of these findings	2306:2345	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	5	40	theme	original	1252:1259	arg1	weight					1266:1271	the original body weight	1248:1271	the original body weight	1248:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	1	41	theme	chronic	389:395	arg1	dosing					397:402	chronic dosing	389:402	chronic dosing	389:402	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	6	42	theme	necrotic	1598:1605	arg1	area					1615:1618	necrotic surface area	1598:1618	3-6% necrotic surface area	1593:1618	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	6	43	dep	necrosis	1583:1590	arg1	%					1596:1596	3-6%	1593:1596	3-6% necrotic surface area	1593:1618	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	2	44	theme	polymeric	583:591	arg1	nanospheres					593:603	polymeric nanospheres	583:603	polymeric nanospheres	583:603	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	5	45	theme	intolerability	1308:1321	arg1	absence					1287:1293	the absence	1283:1293	the absence of long-term intolerability	1283:1321	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	4	46	from	cells	901:905	arg1	levels					861:866	similar levels	853:866	similar levels of apoptotic cell death in tumor cells cultured with splenocytes	853:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	2	47	theme	different	537:545	arg1	compositions					547:558	different compositions	537:558	different compositions (lipid nanoemulsion vs polymeric nanospheres)	537:604	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	8	48	theme	triglyceride	1793:1804	arg1	core					1806:1809	the triglyceride core	1789:1809	the triglyceride core of the nanoemulsion compared to the polymeric matrix	1789:1862	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	2	49	theme	pathogen-mimicking	484:501	arg1	nanostructures					517:530	pathogen-mimicking LPS-decorated nanostructures	484:530	pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm)	484:662	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	5	50	theme	mm3	1431:1433	arg1	volume					1416:1421	a volume	1414:1421	a volume of 1000 mm3	1414:1433	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	9	51	theme	small	1993:1997	arg1	LPS-NE					2017:2022	LPS-NE	2017:2022	LPS-NE (small)	2017:2030	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	9	51	theme	small	1993:1997	arg1	nanoemulsion					2003:2014	small LPS nanoemulsion	1993:2014	small LPS nanoemulsion (LPS-NE (small))	1993:2031	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	12	52	theme	physicochemical	2646:2660	arg1	properties					2662:2671	their physicochemical properties	2640:2671	their physicochemical properties	2640:2671	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	1	53	theme	systemic	306:313	arg1	effects					320:326	severe localized and systemic side effects	285:326	severe localized and systemic side effects	285:326	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	12	54	theme	purposeful	2619:2628	arg1	tuning					2630:2635	a purposeful tuning	2617:2635	a purposeful tuning of their physicochemical properties	2617:2671	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	7	55	theme	Nanostructure	1622:1634	arg1	composition					1636:1646	Nanostructure composition	1622:1646	Nanostructure composition	1622:1646	Nanostructure composition, on the other hand, predominantly governed the long-term systemic tolerability.
31532690	12	56	theme	eventual	2719:2726	arg1	outcome					2746:2752	the eventual immunotherapeutic outcome	2715:2752	the eventual immunotherapeutic outcome	2715:2752	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	10	57	theme	other	2151:2155	arg1	groups					2167:2172	other treatment groups	2151:2172	other treatment groups	2151:2172	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	2	58	theme	LPS	459:461	arg1	molecules					463:471	LPS molecules	459:471	LPS molecules	459:471	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	0	59	theme	Nanostructure-Based	14:32	arg1	Immunotherapy					60:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	4	60	theme	LPS-based	1052:1060	arg1	immunotherapy					1062:1074	LPS-based immunotherapy	1052:1074	LPS-based immunotherapy	1052:1074	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	11	61	theme	immunotherapeutic	2508:2524	arg1	cargos					2526:2531	the incorporated immunotherapeutic cargos	2491:2531	the incorporated immunotherapeutic cargos	2491:2531	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	3	62	theme	cell	697:700	arg1	culture					702:708	cell culture	697:708	cell culture for their immunostimulatory properties	697:747	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	4	63	theme	cell	881:884	arg1	death					886:890	apoptotic cell death	871:890	apoptotic cell death in tumor cells cultured with splenocytes	871:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	0	64	theme	Active	53:58	arg1	Immunotherapy					60:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	5	65	theme	score	1186:1190	arg1	case					1161:1164	the case	1157:1164	the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight	1157:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	10	66	from	%	2196:2196	arg1	LPS-NE					2216:2221	LPS-NE	2216:2221	LPS-NE (small)	2216:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	2	67	theme	lipid	561:565	arg1	nanoemulsion					567:578	lipid nanoemulsion	561:578	lipid nanoemulsion	561:578	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	1	68	theme	administrable	345:357	arg1	dosage					359:364	its administrable dosage	341:364	its administrable dosage	341:364	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	2	69	dep	compositions	547:558	arg1	nanoemulsion					567:578	lipid nanoemulsion	561:578	lipid nanoemulsion	561:578	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	2	69	dep	compositions	547:558	arg1	nanospheres					593:603	polymeric nanospheres	583:603	polymeric nanospheres	583:603	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	5	70	theme	toxicity-related	1089:1104	arg1	point					1110:1114	The toxicity-related end point	1085:1114	The toxicity-related end point of the animal trials	1085:1135	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	3	71	theme	immunostimulatory	720:736	arg1	properties					738:747	their immunostimulatory properties	714:747	their immunostimulatory properties	714:747	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	4	72	theme	similar	853:859	arg1	levels					861:866	similar levels	853:866	similar levels of apoptotic cell death in tumor cells cultured with splenocytes	853:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	5	73	theme	full	1371:1374	arg1	remission					1376:1384	full remission	1371:1384	full remission	1371:1384	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	0	74	dep	Modulation	0:9	arg1	Composition					94:104	Composition	94:104	Composition	94:104	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	0	74	dep	Modulation	0:9	arg1	Size					85:88	Size	85:88	Size	85:88	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	11	75	theme	nanostructures	2450:2463	arg1	role					2442:2445	the role	2438:2445	the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos	2438:2531	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	8	76	theme	nanoemulsion	1818:1829	arg1	core					1806:1809	the triglyceride core	1789:1809	the triglyceride core of the nanoemulsion compared to the polymeric matrix	1789:1862	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	5	77	theme	hair	1212:1215	arg1	coat					1217:1220	1 and poor hair coat	1201:1220	coat	1217:1220	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	9	78	theme	longer	2054:2059	arg1	days					2049:2052	at least 42 days	2037:2052	at least 42 days longer than that of the LPS	2037:2080	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	9	78	theme	longer	2054:2059	arg1	survival					1947:1954	the mean survival	1938:1954	the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small))	1938:2031	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	1	79	theme	localized	292:300	arg1	effects					320:326	severe localized and systemic side effects	285:326	severe localized and systemic side effects	285:326	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	2	80	theme	nm	650:651	arg1	diameters					633:641	volumetric mean diameters	617:641	volumetric mean diameters of 100 nm vs 700 nm	617:661	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	3	81	theme	subcutaneous	778:789	arg1	model					809:813	a murine subcutaneous colorectal cancer model	769:813	a murine subcutaneous colorectal cancer model	769:813	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	8	82	theme	polymeric	1847:1855	arg1	matrix					1857:1862	the polymeric matrix	1843:1862	the polymeric matrix	1843:1862	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	6	83	theme	larger	1503:1508	arg1	particles					1510:1518	larger particles	1503:1518	larger particles	1503:1518	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	2	84	theme	LPS-decorated	503:515	arg1	nanostructures					517:530	pathogen-mimicking LPS-decorated nanostructures	484:530	pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm)	484:662	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	4	85	theme	tumor	895:899	arg1	cells					901:905	tumor cells	895:905	tumor cells cultured with splenocytes	895:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	1	86	theme	strong	205:210	arg1	properties					229:238	the strong pro-inflammatory properties	201:238	the strong pro-inflammatory properties of lipopolysaccharide (LPS)	201:266	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	5	87	dep	loss	1240:1243	arg1	%					1238:1238	%	1238:1238	%	1238:1238	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	6	88	theme	short-term	1473:1482	arg1	tolerability					1484:1495	short-term tolerability	1473:1495	short-term tolerability	1473:1495	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	9	89	dep	survival	1947:1954	arg1	estimate					1956:1963	estimate	1956:1963	estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small))	1956:2031	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	3	90	theme	cancer	802:807	arg1	model					809:813	a murine subcutaneous colorectal cancer model	769:813	a murine subcutaneous colorectal cancer model	769:813	The formulations were tested in cell culture for their immunostimulatory properties and in vivo against a murine subcutaneous colorectal cancer model.
31532690	5	91	theme	trials	1130:1135	arg1	point					1110:1114	The toxicity-related end point	1085:1114	The toxicity-related end point of the animal trials	1085:1135	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	1	92	theme	lipopolysaccharide	243:260	arg1	properties					229:238	the strong pro-inflammatory properties	201:238	the strong pro-inflammatory properties of lipopolysaccharide (LPS)	201:266	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	4	93	theme	nanostructures	975:988	arg1	size					943:946	the size	939:946	the size	939:946	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	4	93	theme	nanostructures	975:988	arg1	composition					956:966	the composition	952:966	the composition of the nanostructures	952:988	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	11	94	theme	larger	2402:2407	arg1	models					2416:2421	larger animal models	2402:2421	larger animal models	2402:2421	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	11	95	from	trial	2393:2397	arg1	models					2416:2421	larger animal models	2402:2421	larger animal models	2402:2421	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	9	96	theme	nanoparticle	2114:2125	arg1	groups					2136:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	8	97	theme	former	1911:1916	arg1	tolerability					1891:1902	the tolerability	1887:1902	the tolerability of the former	1887:1916	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	4	98	from	death	886:890	arg1	cells					901:905	tumor cells	895:905	tumor cells cultured with splenocytes	895:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	12	99	theme	nanoparticle	2588:2599	arg1	composition					2601:2611	nanoparticle composition	2588:2611	nanoparticle composition	2588:2611	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	5	100	theme	body	1261:1264	arg1	weight					1266:1271	the original body weight	1248:1271	the original body weight	1248:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	9	101	theme	LPS-NP	2128:2133	arg1	groups					2136:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	2	102	theme	mean	628:631	arg1	diameters					633:641	volumetric mean diameters	617:641	volumetric mean diameters of 100 nm vs 700 nm	617:661	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	4	103	from	levels	861:866	arg1	cells					901:905	tumor cells	895:905	tumor cells cultured with splenocytes	895:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	7	104	theme	long-term	1695:1703	arg1	tolerability					1714:1725	the long-term systemic tolerability	1691:1725	the long-term systemic tolerability	1691:1725	Nanostructure composition, on the other hand, predominantly governed the long-term systemic tolerability.
31532690	4	105	theme	short-	1015:1020	arg1	tolerability					1036:1047	the short- and long-term tolerability	1011:1047	the short- and long-term tolerability of LPS-based immunotherapy	1011:1074	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	5	106	theme	loss	1240:1243	arg1	BCS					1193:1195	BCS	1193:1195	BCS	1193:1195	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	5	106	theme	loss	1240:1243	arg1	score					1186:1190	a body condition score	1169:1190	a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight	1169:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	12	107	theme	immunotherapeutic	2728:2744	arg1	outcome					2746:2752	the eventual immunotherapeutic outcome	2715:2752	the eventual immunotherapeutic outcome	2715:2752	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	8	108	theme	higher	1753:1758	arg1	affinity					1760:1767	the higher affinity	1749:1767	the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix	1749:1862	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	1	109	theme	cancer	179:184	arg1	immunotherapy					186:198	cancer immunotherapy	179:198	cancer immunotherapy	179:198	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	4	110	theme	long-term	1026:1034	arg1	tolerability					1036:1047	the short- and long-term tolerability	1011:1047	the short- and long-term tolerability of LPS-based immunotherapy	1011:1074	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	0	111	theme	Short-	116:121	arg1	Tolerability					137:148	Short- and Long-Term Tolerability	116:148	Short- and Long-Term Tolerability	116:148	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	5	112	theme	condition	1176:1184	arg1	BCS					1193:1195	BCS	1193:1195	BCS	1193:1195	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	5	112	theme	condition	1176:1184	arg1	score					1186:1190	a body condition score	1169:1190	a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight	1169:1271	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	6	113	theme	surface	1607:1613	arg1	area					1615:1618	necrotic surface area	1598:1618	3-6% necrotic surface area	1593:1618	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	10	114	theme	>1000	2289:2293	arg1	mm3					2295:2297	tumor volume >1000 mm3	2276:2297	tumor volume >1000 mm3	2276:2297	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	5	115	theme	long-term	1298:1306	arg1	intolerability					1308:1321	long-term intolerability	1298:1321	long-term intolerability	1298:1321	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	0	116	theme	Long-Term	127:135	arg1	Tolerability					137:148	Short- and Long-Term Tolerability	116:148	Short- and Long-Term Tolerability	116:148	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	1	117	theme	dosing	397:402	arg1	possibility					374:384	the possibility	370:384	the possibility of chronic dosing	370:402	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	1	117	theme	dosing	397:402	arg1	dosage					359:364	its administrable dosage	341:364	its administrable dosage	341:364	Despite holding promise for cancer immunotherapy, the strong pro-inflammatory properties of lipopolysaccharide (LPS) also account for severe localized and systemic side effects, restricting its administrable dosage and the possibility of chronic dosing.
31532690	8	118	theme	molecules	1776:1784	arg1	affinity					1760:1767	the higher affinity	1749:1767	the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix	1749:1862	Within this context, the higher affinity of LPS molecules to the triglyceride core of the nanoemulsion compared to the polymeric matrix significantly improved the tolerability of the former over time.
31532690	7	119	theme	other	1656:1660	arg1	hand					1662:1665	the other hand	1652:1665	the other hand	1652:1665	Nanostructure composition, on the other hand, predominantly governed the long-term systemic tolerability.
31532690	12	120	theme	composition	2601:2611	arg1	selection					2575:2583	a wise selection	2568:2583	a wise selection of nanoparticle composition	2568:2611	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	10	121	theme	tumor	2276:2280	arg1	mm3					2295:2297	tumor volume >1000 mm3	2276:2297	tumor volume >1000 mm3	2276:2297	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	10	122	theme	volume	2282:2287	arg1	mm3					2295:2297	tumor volume >1000 mm3	2276:2297	tumor volume >1000 mm3	2276:2297	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	9	123	theme	LPS-decorated	2090:2102	arg1	groups					2136:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	the LPS-decorated polymeric nanoparticle (LPS-NP) groups	2086:2141	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	12	124	theme	safety	2688:2693	arg1	profile					2695:2701	the safety profile	2684:2701	the safety profile	2684:2701	This highlights the importance of a wise selection of nanoparticle composition and a purposeful tuning of their physicochemical properties to enhance the safety profile and improve the eventual immunotherapeutic outcome.
31532690	10	125	from	experiments	2179:2189	arg1	%					2196:2196	80%	2194:2196	80% of the animals in LPS-NE (small)	2194:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	10	125	from	experiments	2179:2189	arg1	animals					2205:2211	the animals	2201:2211	the animals in LPS-NE (small)	2201:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	9	126	theme	LPS	1999:2001	arg1	LPS-NE					2017:2022	LPS-NE	2017:2022	LPS-NE (small)	2017:2030	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	9	126	theme	LPS	1999:2001	arg1	nanoemulsion					2003:2014	small LPS nanoemulsion	1993:2014	small LPS nanoemulsion (LPS-NE (small))	1993:2031	In fact, the mean survival estimate of the animals treated with small LPS nanoemulsion (LPS-NE (small)) was at least 42 days longer than that of the LPS and the LPS-decorated polymeric nanoparticle (LPS-NP) groups.
31532690	0	127	theme	Lipopolysaccharide	34:51	arg1	Immunotherapy					60:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Nanostructure-Based Lipopolysaccharide Active Immunotherapy	14:72	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	5	128	theme	1	1201:1201	arg1	coat					1217:1220	1 and poor hair coat	1201:1220	coat	1217:1220	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	6	129	theme	localized	1573:1581	arg1	necrosis					1583:1590	localized necrosis	1573:1590	localized necrosis (3-6% necrotic surface area)	1573:1619	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	0	130	dep	Size	85:88	arg1	Determine					106:114	Determine	106:114	Determine Short- and Long-Term Tolerability	106:148	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	6	131	dep	%	1596:1596	arg1	area					1615:1618	necrotic surface area	1598:1618	3-6% necrotic surface area	1593:1618	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	2	132	theme	molecules	463:471	arg1	properties					445:454	the surface-active properties	426:454	the surface-active properties of LPS molecules	426:471	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	11	133	theme	incorporated	2495:2506	arg1	cargos					2526:2531	the incorporated immunotherapeutic cargos	2491:2531	the incorporated immunotherapeutic cargos	2491:2531	While a better understanding of these findings requires a larger scale, mechanistic-oriented trial on larger animal models, they indicate the role of nanostructures as beyond the carriers of the incorporated immunotherapeutic cargos.
31532690	0	134	theme	Immunotherapy	60:72	arg1	Modulation					0:9	Modulation	0:9	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.	0:149	Modulation of Nanostructure-Based Lipopolysaccharide Active Immunotherapy in Cancer: Size and Composition Determine Short- and Long-Term Tolerability.
31532690	5	135	theme	poor	1207:1210	arg1	coat					1217:1220	1 and poor hair coat	1201:1220	coat	1217:1220	The toxicity-related end point of the animal trials was decided upon in the case of a body condition score (BCS) of 1 and poor hair coat, or more than 15% loss of the original body weight, while in the absence of long-term intolerability, the experiments were terminated in the case of full remission or once the tumor surpassed a volume of 1000 mm3.
31532690	4	136	theme	apoptotic	871:879	arg1	death					886:890	apoptotic cell death	871:890	apoptotic cell death in tumor cells cultured with splenocytes	871:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	2	137	theme	surface-active	430:443	arg1	properties					445:454	the surface-active properties	426:454	the surface-active properties of LPS molecules	426:471	Herein, we exploited the surface-active properties of LPS molecules to develop pathogen-mimicking LPS-decorated nanostructures with different compositions (lipid nanoemulsion vs polymeric nanospheres) and sizes (volumetric mean diameters of 100 nm vs 700 nm).
31532690	4	138	theme	immunotherapy	1062:1074	arg1	tolerability					1036:1047	the short- and long-term tolerability	1011:1047	the short- and long-term tolerability of LPS-based immunotherapy	1011:1074	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	10	139	from	animals	2205:2211	arg1	LPS-NE					2216:2221	LPS-NE	2216:2221	LPS-NE (small)	2216:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	4	140	theme	death	886:890	arg1	levels					861:866	similar levels	853:866	similar levels of apoptotic cell death in tumor cells cultured with splenocytes	853:931	While all nanostructures resulted in similar levels of apoptotic cell death in tumor cells cultured with splenocytes, both the size and the composition of the nanostructures were found to govern the short- and long-term tolerability of LPS-based immunotherapy in vivo.
31532690	6	141	theme	important	1448:1456	arg1	determinant					1458:1468	an important determinant	1445:1468	an important determinant of short-term tolerability	1445:1495	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	6	141	theme	important	1448:1456	arg1	Size					1436:1439	Size	1436:1439	Size	1436:1439	Size was an important determinant of short-term tolerability, with larger particles being associated with higher incidence and extent of localized necrosis (3-6% necrotic surface area).
31532690	10	142	from	LPS-NE	2216:2221	arg1	%					2196:2196	80%	2194:2196	80% of the animals in LPS-NE (small)	2194:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
31532690	10	142	from	LPS-NE	2216:2221	arg1	animals					2205:2211	the animals	2201:2211	the animals in LPS-NE (small)	2201:2229	Unlike other treatment groups, the experiments on 80% of the animals in LPS-NE (small) were terminated due to complete remission or tumor volume >1000 mm3.
30241399	2	0	theme	monomer	376:382	arg1	presence					347:354	the presence	343:354	the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate	343:454	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	7	1	theme	natural	1492:1498	arg1	ageing					1500:1505	natural ageing	1492:1505	natural ageing	1492:1505	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	0	2	theme	Based	77:81	arg1	Eco-Composites					83:96	Natural Rubber and Plasticized Potato Starch Based Eco-Composites	32:96	Eco-Composites	83:96	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	3	3	theme	gel	559:561	arg1	fraction					563:570	gel fraction	559:570	gel fraction	559:570	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	3	theme	gel	559:561	arg1	composites					507:516	the composites physical and mechanical characteristics	503:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	5	4	from	ageing	1105:1110	arg1	climate					1125:1131	temperate climate	1115:1131	temperate climate	1115:1131	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	3	5	theme	temperate	716:724	arg1	climate					726:732	temperate climate	716:732	temperate climate	716:732	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	2	6	theme	polyfunctional	361:374	arg1	monomer					376:382	a polyfunctional monomer	359:382	a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate	359:454	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	1	7	dep	obtaining	138:146	arg1	The					134:136	The	134:136	The	134:136	The obtaining and characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler, are presented.
30241399	5	8	theme	mass	983:986	arg1	loss					988:991	mass loss	983:991	mass loss	983:991	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	5	9	theme	temperate	1115:1123	arg1	climate					1125:1131	temperate climate	1115:1131	temperate climate	1115:1131	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	4	10	theme	orders	775:780	arg1	Differences					756:766	Differences	756:766	Differences of two orders of magnitude between the degradation/aging methods	756:831	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	3	11	theme	water	596:600	arg1	uptake					602:607	water uptake	596:607	water uptake	596:607	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	6	12	theme	accelerated	1299:1309	arg1	ageing					1323:1328	accelerated and natural ageing	1299:1328	accelerated and natural ageing	1299:1328	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	3	13	theme	cross-link	576:585	arg1	density					587:593	cross-link density	576:593	cross-link density	576:593	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	13	theme	cross-link	576:585	arg1	composites					507:516	the composites physical and mechanical characteristics	503:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	1	14	theme	composites	200:209	arg1	characterization					152:167	characterization	152:167	characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler,	152:277	The obtaining and characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler, are presented.
30241399	0	15	theme	Peroxide	110:117	arg1	Cross-Linking					119:131	Peroxide Cross-Linking	110:131	Peroxide Cross-Linking	110:131	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	4	16	theme	magnitude	785:793	arg1	orders					775:780	two orders	771:780	two orders of magnitude	771:793	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	3	17	theme	starch	486:491	arg1	amount					493:498	plasticized starch amount	474:498	plasticized starch amount	474:498	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	18	from	year	708:711	arg1	climate					726:732	temperate climate	716:732	temperate climate	716:732	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	4	19	theme	characteristics	880:894	arg1	case					856:859	the case	852:859	the case of some mechanical characteristics	852:894	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	5	20	theme	water	966:970	arg1	uptake					972:977	water uptake	966:977	water uptake	966:977	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	2	21	theme	cross-linking	397:409	arg1	co-agent					411:418	cross-linking co-agent	397:418	cross-linking co-agent	397:418	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	2	21	theme	cross-linking	397:409	arg1	trimethacrylate					440:454	trimethylolpropane trimethacrylate	421:454	trimethylolpropane trimethacrylate	421:454	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	0	22	theme	Degradation	0:10	arg1	Studies					12:18	Degradation Studies	0:18	Degradation Studies	0:18	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	4	23	theme	mechanical	869:878	arg1	characteristics					880:894	some mechanical characteristics	864:894	some mechanical characteristics	864:894	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	6	24	theme	Infrared	1176:1183	arg1	FTIR					1199:1202	FTIR	1199:1202	FTIR	1199:1202	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	6	24	theme	Infrared	1176:1183	arg1	Spectroscopy					1185:1196	Infrared Spectroscopy	1176:1196	Infrared Spectroscopy (FTIR)	1176:1203	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	5	25	theme	natural	1097:1103	arg1	ageing					1105:1110	natural ageing	1097:1110	natural ageing in temperate climate	1097:1131	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	6	26	theme	density	1220:1226	arg1	measurements					1228:1239	cross-link density measurements	1209:1239	cross-link density measurements	1209:1239	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	2	27	theme	peroxide	317:324	arg1	cross-linking					326:338	peroxide cross-linking	317:338	peroxide cross-linking	317:338	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	7	28	theme	hydrophilic	1420:1430	arg1	amount					1439:1444	hydrophilic starch amount	1420:1444	hydrophilic starch amount	1420:1444	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	7	29	theme	SEM	1341:1343	arg1	micrographs					1345:1355	SEM micrographs	1341:1355	SEM micrographs	1341:1355	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	7	30	theme	thermal	1565:1571	arg1	degradation					1573:1583	thermal degradation	1565:1583	thermal degradation	1565:1583	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	1	31	theme	environmental-friendly	177:198	arg1	composites					200:209	some environmental-friendly composites	172:209	some environmental-friendly composites	172:209	The obtaining and characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler, are presented.
30241399	1	32	theme	natural	229:235	arg1	rubber					237:242	natural rubber	229:242	natural rubber	229:242	The obtaining and characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler, are presented.
30241399	3	33	theme	natural	691:697	arg1	ageing					735:740	natural (for one year in temperate climate) ageing	691:740	natural (for one year in temperate climate) ageing	691:740	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	5	34	theme	starch	1043:1048	arg1	amount					1050:1055	the plasticized starch amount	1027:1055	the plasticized starch amount	1027:1055	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	6	35	theme	cross-link	1209:1218	arg1	measurements					1228:1239	cross-link density measurements	1209:1239	cross-link density measurements	1209:1239	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	3	36	theme	amount	493:498	arg1	influence					461:469	The influence	457:469	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing,	457:741	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	0	37	theme	Natural	32:38	arg1	Rubber					40:45	Natural Rubber and Plasticized Potato Starch Based Eco-Composites	32:96	Rubber	40:45	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	7	38	theme	starch	1432:1437	arg1	amount					1439:1444	hydrophilic starch amount	1420:1444	hydrophilic starch amount	1420:1444	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	2	39	theme	trimethylolpropane	421:438	arg1	co-agent					411:418	cross-linking co-agent	397:418	cross-linking co-agent	397:418	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	2	39	theme	trimethylolpropane	421:438	arg1	trimethacrylate					440:454	trimethylolpropane trimethacrylate	421:454	trimethylolpropane trimethacrylate	421:454	These were obtained by peroxide cross-linking in the presence of a polyfunctional monomer used here as cross-linking co-agent, trimethylolpropane trimethacrylate.
30241399	0	40	theme	Plasticized	51:61	arg1	Eco-Composites					83:96	Natural Rubber and Plasticized Potato Starch Based Eco-Composites	32:96	Eco-Composites	83:96	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	4	41	theme	starch	927:932	arg1	amount					934:939	the plasticized starch amount	911:939	the plasticized starch amount	911:939	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	3	42	theme	plasticized	474:484	arg1	amount					493:498	plasticized starch amount	474:498	plasticized starch amount	474:498	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	43	theme	mechanical	531:540	arg1	characteristics					542:556	physical and mechanical characteristics	518:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	4	44	theme	plasticized	915:925	arg1	amount					934:939	the plasticized starch amount	911:939	the plasticized starch amount	911:939	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	6	45	theme	reaction	1242:1249	arg1	mechanisms					1251:1260	reaction mechanisms	1242:1260	reaction mechanisms attributed to degradation induced by accelerated and natural ageing	1242:1328	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	5	46	theme	cross-link	946:955	arg1	density					957:963	The cross-link density	942:963	The cross-link density	942:963	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	5	47	theme	plasticized	1031:1041	arg1	amount					1050:1055	the plasticized starch amount	1027:1055	the plasticized starch amount	1027:1055	The cross-link density, water uptake and mass loss were also significant affected by the plasticized starch amount increasing and exposing for one year to natural ageing in temperate climate.
30241399	4	48	theme	degradation/aging	807:823	arg1	methods					825:831	the degradation/aging methods	803:831	the degradation/aging methods	803:831	Differences of two orders of magnitude between the degradation/aging methods were registered in the case of some mechanical characteristics, by increasing the plasticized starch amount.
30241399	3	49	theme	thermal	665:671	arg1	degradation					674:684	accelerated (thermal) degradation	652:684	accelerated (thermal) degradation	652:684	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	50	theme	physical	518:525	arg1	characteristics					542:556	physical and mechanical characteristics	518:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	7	51	theme	amount	1439:1444	arg1	quantity					1408:1415	a quantity	1406:1415	a quantity of hydrophilic starch amount	1406:1444	SEM micrographs have confirmed in addition that by incorporating a quantity of hydrophilic starch amount over 20 phr and by exposing the composites to natural ageing, and then degradability can be enhanced by comparing with thermal degradation.
30241399	1	52	theme	plasticized	248:258	arg1	starch					260:265	plasticized starch	248:265	plasticized starch	248:265	The obtaining and characterization of some environmental-friendly composites that are based on natural rubber and plasticized starch, as filler, are presented.
30241399	3	53	from	influence	461:469	arg1	fraction					563:570	gel fraction	559:570	gel fraction	559:570	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	53	from	influence	461:469	arg1	density					587:593	cross-link density	576:593	cross-link density	576:593	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	3	53	from	influence	461:469	arg1	composites					507:516	the composites physical and mechanical characteristics	503:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	0	54	theme	Starch	70:75	arg1	Eco-Composites					83:96	Natural Rubber and Plasticized Potato Starch Based Eco-Composites	32:96	Eco-Composites	83:96	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	3	55	theme	accelerated	652:662	arg1	degradation					674:684	accelerated (thermal) degradation	652:684	accelerated (thermal) degradation	652:684	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	6	56	dep	Fourier	1158:1164	arg1	Transform					1166:1174	Transform	1166:1174	Transform Infrared Spectroscopy (FTIR) and cross-link density measurements	1166:1239	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	0	57	theme	Potato	63:68	arg1	Eco-Composites					83:96	Natural Rubber and Plasticized Potato Starch Based Eco-Composites	32:96	Eco-Composites	83:96	Degradation Studies Realized on Natural Rubber and Plasticized Potato Starch Based Eco-Composites Obtained by Peroxide Cross-Linking.
30241399	6	58	theme	Fourier	1158:1164	arg1	results					1147:1153	the results	1143:1153	the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements	1143:1239	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30241399	3	59	dep	composites	507:516	arg1	characteristics					542:556	physical and mechanical characteristics	518:556	the composites physical and mechanical characteristics	503:556	The influence of plasticized starch amount on the composites physical and mechanical characteristics, gel fraction and cross-link density, water uptake, structure and morphology before and after accelerated (thermal) degradation, and natural (for one year in temperate climate) ageing, was studied.
30241399	6	60	theme	natural	1315:1321	arg1	ageing					1323:1328	accelerated and natural ageing	1299:1328	accelerated and natural ageing	1299:1328	Based on the results of Fourier Transform Infrared Spectroscopy (FTIR) and cross-link density measurements, reaction mechanisms attributed to degradation induced by accelerated and natural ageing were done.
30296361	2	0	theme	wt	590:591	arg1	%					593:593	2, 3, and 1 wt %	578:593	2, 3, and 1 wt %	578:593	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	1	theme	carboxymethylcellulose	358:379	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	1	theme	carboxymethylcellulose	358:379	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	4	2	theme	properties	835:844	arg1	Analysis					810:817	Analysis	810:817	Analysis of the physical properties of this dressing	810:861	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	9	3	dep	Res	1804:1806	arg1	B					1813:1813	Part B	1808:1813	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.	1789:1851	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.
30296361	9	3	dep	Res	1804:1806	arg1	Biomater					1821:1828	Biomater	1821:1828	Biomater	1821:1828	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.
30296361	9	3	dep	Res	1804:1806	arg1	2019					1847:1850	2019	1847:1850	2019	1847:1850	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.
30296361	7	4	theme	clinical	1735:1742	arg1	applications					1744:1755	clinical applications	1735:1755	clinical applications	1735:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	1	5	theme	wound	195:199	arg1	healing					201:207	wound healing	195:207	wound healing	195:207	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	7	6	theme	anti-adhesive	1598:1610	arg1	properties					1612:1621	excellent self-regulatory and anti-adhesive properties	1568:1621	excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications	1568:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	2	7	theme	CS	572:573	arg1	ratio					547:551	an optimal ratio	536:551	an optimal ratio of CMC-Na, SA, and CS	536:573	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	6	8	theme	wound	1458:1462	arg1	healing					1464:1470	wound healing	1458:1470	the wound healing process	1454:1478	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	7	9	theme	self-regulatory	1578:1592	arg1	properties					1612:1621	excellent self-regulatory and anti-adhesive properties	1568:1621	excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications	1568:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	4	10	theme	vapor	900:904	arg1	properties					919:928	good water vapor permeability properties	889:928	good water vapor permeability properties that promote the healing of deep second-degree burn wounds	889:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	3	11	used	used	724:727	arg2	dressings					699:707	composite wound dressings	683:707	composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats	683:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	3	12	theme	second	755:760	arg1	wounds					774:779	deep second degree burn wounds	750:779	deep second degree burn wounds in Sprague-Dawley (SD) rats	750:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	5	13	theme	endothelial	1063:1073	arg1	VEGF					1090:1093	VEGF	1090:1093	VEGF	1090:1093	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	13	theme	endothelial	1063:1073	arg1	factor					1082:1087	Vascular endothelial growth factor	1054:1087	Vascular endothelial growth factor (VEGF) expression	1054:1105	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	6	14	theme	necrosis	1376:1383	arg1	levels					1360:1365	the levels	1356:1365	the levels of tumor necrosis factor-α and interleukin-6	1356:1410	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	7	15	theme	promising	1703:1711	arg1	results					1713:1719	promising results	1703:1719	promising results that may have clinical applications	1703:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	5	16	theme	bFGF	1254:1257	arg1	upregulation					1238:1249	upregulation	1238:1249	upregulation of bFGF	1238:1257	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	2	17	theme	optimal	539:545	arg1	ratio					547:551	an optimal ratio	536:551	an optimal ratio of CMC-Na, SA, and CS	536:573	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	4	18	theme	good	889:892	arg1	properties					919:928	good water vapor permeability properties	889:928	good water vapor permeability properties that promote the healing of deep second-degree burn wounds	889:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	3	19	theme	burn	769:772	arg1	wounds					774:779	deep second degree burn wounds	750:779	deep second degree burn wounds in Sprague-Dawley (SD) rats	750:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	6	20	theme	wound	1331:1335	arg1	dressing					1337:1344	the wound dressing	1327:1344	the wound dressing	1327:1344	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	0	21	theme	self-regulating/anti-adhesive	15:43	arg1	hydrogels					45:53	self-regulating/anti-adhesive hydrogels	15:53	self-regulating/anti-adhesive hydrogels	15:53	Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.
30296361	1	22	theme	humidity	157:164	arg1	levels					166:171	the optimal humidity levels	145:171	the optimal humidity levels that are required for wound healing	145:207	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	7	23	theme	new	1525:1527	arg1	dressing					1538:1545	this new hydrogel dressing	1520:1545	this new hydrogel dressing	1520:1545	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	4	24	theme	deep	958:961	arg1	wounds					982:987	deep second-degree burn wounds	958:987	deep second-degree burn wounds	958:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	7	25	theme	wounds	1672:1677	arg1	healing					1656:1662	the healing	1652:1662	the healing of burn wounds in rats	1652:1685	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	6	26	theme	same	1316:1319	arg1	time					1321:1324	the same time	1312:1324	the same time	1312:1324	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	5	27	theme	factor	1082:1087	arg1	expression					1096:1105	Vascular endothelial growth factor (VEGF) expression	1054:1105	Vascular endothelial growth factor (VEGF) expression	1054:1105	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	28	theme	wound	1294:1298	arg1	healing					1300:1306	wound healing	1294:1306	wound healing	1294:1306	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	4	29	theme	burn	977:980	arg1	wounds					982:987	deep second-degree burn wounds	958:987	deep second-degree burn wounds	958:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	2	30	theme	wound	486:490	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	30	theme	wound	486:490	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	31	theme	potential	471:479	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	31	theme	potential	471:479	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	6	32	theme	tumor	1370:1374	arg1	necrosis					1376:1383	tumor necrosis factor-α and interleukin-6	1370:1410	necrosis	1376:1383	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	1	33	theme	burn	112:115	arg1	dressings					117:125	Few burn dressings	108:125	Few burn dressings	108:125	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	2	34	theme	orthogonal	507:516	arg1	testing					518:524	orthogonal testing	507:524	orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively	507:632	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	5	35	theme	basic	1127:1131	arg1	bFGF					1159:1162	bFGF	1159:1162	bFGF	1159:1162	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	35	theme	basic	1127:1131	arg1	factor					1151:1156	basic fibroblast growth factor	1127:1156	basic fibroblast growth factor (bFGF) expression	1127:1174	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	36	theme	pro-healing	994:1004	arg1	mechanism					1006:1014	The pro-healing mechanism	990:1014	The pro-healing mechanism of the dressing	990:1030	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	2	37	theme	SA	564:565	arg1	ratio					547:551	an optimal ratio	536:551	an optimal ratio of CMC-Na, SA, and CS	536:573	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	38	theme	water-soluble	344:356	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	38	theme	water-soluble	344:356	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	5	39	theme	growth	1144:1149	arg1	bFGF					1159:1162	bFGF	1159:1162	bFGF	1159:1162	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	39	theme	growth	1144:1149	arg1	factor					1151:1156	basic fibroblast growth factor	1127:1156	basic fibroblast growth factor (bFGF) expression	1127:1174	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	6	40	from	effect	1444:1449	arg1	process					1472:1478	the wound healing process	1454:1478	the wound healing process	1454:1478	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	9	41	theme	Part	1808:1811	arg1	B					1813:1813	Part B	1808:1813	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.	1789:1851	J Biomed Mater Res Part B: Appl Biomater 107B: 1471-1482, 2019.
30296361	5	42	theme	early	1201:1205	arg1	periods					1207:1213	the early periods	1197:1213	the early periods of wound healing	1197:1230	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	1	43	theme	anti-adhesive	236:248	arg1	properties					250:259	good anti-adhesive properties	231:259	good anti-adhesive properties	231:259	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	3	44	theme	wounds	774:779	arg1	treatment					737:745	the treatment	733:745	the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats	733:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	3	45	theme	wound	693:697	arg1	dressings					699:707	composite wound dressings	683:707	composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats	683:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	2	46	theme	sodium/sodium	381:393	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	46	theme	sodium/sodium	381:393	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	4	47	theme	physical	826:833	arg1	properties					835:844	the physical properties	822:844	the physical properties of this dressing	822:861	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	6	48	theme	interleukin-6	1398:1410	arg1	levels					1360:1365	the levels	1356:1365	the levels of tumor necrosis factor-α and interleukin-6	1356:1410	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	6	49	theme	biomolecular	1485:1496	arg1	level					1498:1502	a biomolecular level	1483:1502	a biomolecular level	1483:1502	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	2	50	theme	hydrogel	599:606	arg1	preparation					608:618	hydrogel preparation	599:618	hydrogel preparation	599:618	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	6	51	theme	healing	1464:1470	arg1	process					1472:1478	the wound healing process	1454:1478	the wound healing process	1454:1478	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	7	52	theme	excellent	1568:1576	arg1	properties					1612:1621	excellent self-regulatory and anti-adhesive properties	1568:1621	excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications	1568:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	5	53	theme	Vascular	1054:1061	arg1	VEGF					1090:1093	VEGF	1090:1093	VEGF	1090:1093	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	53	theme	Vascular	1054:1061	arg1	factor					1082:1087	Vascular endothelial growth factor	1054:1087	Vascular endothelial growth factor (VEGF) expression	1054:1105	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	4	54	theme	water	894:898	arg1	properties					919:928	good water vapor permeability properties	889:928	good water vapor permeability properties that promote the healing of deep second-degree burn wounds	889:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	0	55	from	healing	84:90	arg1	wounds					100:105	burn wounds	95:105	burn wounds	95:105	Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.
30296361	3	56	theme	degree	762:767	arg1	wounds					774:779	deep second degree burn wounds	750:779	deep second degree burn wounds in Sprague-Dawley (SD) rats	750:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	5	57	theme	growth	1075:1080	arg1	VEGF					1090:1093	VEGF	1090:1093	VEGF	1090:1093	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	57	theme	growth	1075:1080	arg1	factor					1082:1087	Vascular endothelial growth factor	1054:1087	Vascular endothelial growth factor (VEGF) expression	1054:1105	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	4	58	theme	dressing	854:861	arg1	properties					835:844	the physical properties	822:844	the physical properties of this dressing	822:861	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	3	59	theme	deep	750:753	arg1	wounds					774:779	deep second degree burn wounds	750:779	deep second degree burn wounds in Sprague-Dawley (SD) rats	750:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	5	60	theme	dressing	1023:1030	arg1	mechanism					1006:1014	The pro-healing mechanism	990:1014	The pro-healing mechanism of the dressing	990:1030	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	7	61	theme	hydrogel	1529:1536	arg1	dressing					1538:1545	this new hydrogel dressing	1520:1545	this new hydrogel dressing	1520:1545	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	7	62	from	healing	1656:1662	arg1	rats					1682:1685	rats	1682:1685	rats	1682:1685	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	3	63	theme	Sprague-Dawley	784:797	arg1	rats					804:807	Sprague-Dawley (SD) rats	784:807	Sprague-Dawley (SD) rats	784:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	1	64	theme	optimal	149:155	arg1	levels					166:171	the optimal humidity levels	145:171	the optimal humidity levels that are required for wound healing	145:207	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	5	65	theme	healing	1224:1230	arg1	periods					1207:1213	the early periods	1197:1213	the early periods of wound healing	1197:1230	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	4	66	theme	permeability	906:917	arg1	properties					919:928	good water vapor permeability properties	889:928	good water vapor permeability properties that promote the healing of deep second-degree burn wounds	889:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	0	67	theme	burn	95:98	arg1	wounds					100:105	burn wounds	95:105	burn wounds	95:105	Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.
30296361	2	68	theme	CMC-Na	556:561	arg1	ratio					547:551	an optimal ratio	536:551	an optimal ratio of CMC-Na, SA, and CS	536:573	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	6	69	from	level	1498:1502	arg1	effect					1444:1449	its beneficial effect	1429:1449	its beneficial effect on the wound healing process at a biomolecular level	1429:1502	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	7	70	theme	burn	1667:1670	arg1	wounds					1672:1677	burn wounds	1667:1677	burn wounds	1667:1677	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	5	71	theme	healing	1300:1306	arg1	stage					1285:1289	a later stage	1277:1289	a later stage of wound healing	1277:1306	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	2	72	theme	composite	428:436	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	72	theme	composite	428:436	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	73	theme	burn	481:484	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	73	theme	burn	481:484	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	3	74	from	treatment	737:745	arg1	rats					804:807	Sprague-Dawley (SD) rats	784:807	Sprague-Dawley (SD) rats	784:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	2	75	theme	CMC-Na/SA/CS	414:425	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	75	theme	CMC-Na/SA/CS	414:425	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	0	76	theme	hydrogels	45:53	arg1	Preparation					0:10	Preparation	0:10	Preparation of self-regulating/anti-adhesive hydrogels	0:53	Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.
30296361	0	76	theme	hydrogels	45:53	arg1	ability					65:71	their ability	59:71	their ability	59:71	Preparation of self-regulating/anti-adhesive hydrogels and their ability to promote healing in burn wounds.
30296361	1	77	theme	Few	108:110	arg1	dressings					117:125	Few burn dressings	108:125	Few burn dressings	108:125	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	7	78	contain	have	1730:1733	arg2	applications					1744:1755	clinical applications	1735:1755	clinical applications	1735:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	7	78	contain	have	1730:1733	arg1	results					1713:1719	promising results	1703:1719	promising results that may have clinical applications	1703:1755	In conclusion, this new hydrogel dressing was shown to exhibit excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats, thus providing promising results that may have clinical applications.
30296361	5	79	theme	later	1279:1283	arg1	stage					1285:1289	a later stage	1277:1289	a later stage of wound healing	1277:1306	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	1	80	theme	wound	299:303	arg1	dressings					305:313	wound dressings	299:313	wound dressings	299:313	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	4	81	theme	second-degree	963:975	arg1	wounds					982:987	deep second-degree burn wounds	958:987	deep second-degree burn wounds	958:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	3	82	from	rats	804:807	arg1	treatment					737:745	the treatment	733:745	the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats	733:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	5	83	theme	fibroblast	1133:1142	arg1	bFGF					1159:1162	bFGF	1159:1162	bFGF	1159:1162	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	5	83	theme	fibroblast	1133:1142	arg1	factor					1151:1156	basic fibroblast growth factor	1127:1156	basic fibroblast growth factor (bFGF) expression	1127:1174	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	6	84	dep	necrosis	1376:1383	arg1	factor-α					1385:1392	factor-α	1385:1392	factor-α	1385:1392	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	4	85	theme	wounds	982:987	arg1	healing					947:953	the healing	943:953	the healing of deep second-degree burn wounds	943:987	Analysis of the physical properties of this dressing revealed that it exhibits good water vapor permeability properties that promote the healing of deep second-degree burn wounds.
30296361	3	86	from	wounds	774:779	arg1	rats					804:807	Sprague-Dawley (SD) rats	784:807	Sprague-Dawley (SD) rats	784:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	5	87	theme	factor	1151:1156	arg1	expression					1165:1174	basic fibroblast growth factor (bFGF) expression	1127:1174	basic fibroblast growth factor (bFGF) expression	1127:1174	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
30296361	8	88	dep	©	1758:1758	arg1	Inc.					1784:1787	Inc.	1784:1787	Inc.	1784:1787	© 2018 Wiley Periodicals, Inc.
30296361	3	89	theme	resultant	639:647	arg1	hydrogel					649:656	The resultant hydrogel	635:656	The resultant hydrogel	635:656	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	6	90	theme	beneficial	1433:1442	arg1	effect					1444:1449	its beneficial effect	1429:1449	its beneficial effect on the wound healing process at a biomolecular level	1429:1502	At the same time, the wound dressing decreased the levels of tumor necrosis factor-α and interleukin-6, thus validating its beneficial effect on the wound healing process at a biomolecular level.
30296361	1	91	theme	good	231:234	arg1	properties					250:259	good anti-adhesive properties	231:259	good anti-adhesive properties	231:259	Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed.
30296361	3	92	theme	composite	683:691	arg1	dressings					699:707	composite wound dressings	683:707	composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats	683:807	The resultant hydrogel has been formulated into composite wound dressings that were then used for the treatment of deep second degree burn wounds in Sprague-Dawley (SD) rats.
30296361	2	93	theme	alginate/chitosan	395:411	arg1	hydrogel					438:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel	342:445	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	2	93	theme	alginate/chitosan	395:411	arg1	dressing					492:499	a potential burn wound dressing	469:499	a potential burn wound dressing	469:499	Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively.
30296361	5	94	theme	wound	1218:1222	arg1	healing					1224:1230	wound healing	1218:1230	wound healing	1218:1230	The pro-healing mechanism of the dressing has been investigated Vascular endothelial growth factor (VEGF) expression was upregulated and basic fibroblast growth factor (bFGF) expression was downregulated in the early periods of wound healing, with upregulation of bFGF then occurring at a later stage of wound healing.
31654152	7	0	theme	solution	1182:1189	arg1	height					1196:1201	solution fill height	1182:1201	solution fill height	1182:1201	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	1	1	theme	biomaterial	150:160	arg1	scaffolds					162:170	biomaterial scaffolds	150:170	biomaterial scaffolds with highly porous structures	150:200	Freeze-casting is a popular method to produce biomaterial scaffolds with highly porous structures.
31654152	3	2	theme	mathematical	347:358	arg1	model					360:364	A mathematical model	345:364	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model	345:435	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	1	3	with	scaffolds	162:170	arg1	structures					191:200	highly porous structures	177:200	highly porous structures	177:200	Freeze-casting is a popular method to produce biomaterial scaffolds with highly porous structures.
31654152	7	4	theme	mold	1163:1166	arg1	radius					1174:1179	the mold: well radius	1159:1179	the mold: well radius	1159:1179	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	7	5	theme	height	1196:1201	arg1	parameters					1145:1154	the three geometrical parameters	1123:1154	the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells	1123:1228	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	9	6	theme	other	1466:1470	arg1	compositions					1472:1483	compositions	1472:1483	compositions	1472:1483	This validated model demonstrates a method for optimizing the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications.
31654152	0	7	from	Effect	0:5	arg1	Size					32:35	Pore Size	27:35	Pore Size in Freeze-Cast Chitosan-Alginate	27:68	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	7	8	theme	radius	1174:1179	arg1	parameters					1145:1154	the three geometrical parameters	1123:1154	the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells	1123:1228	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	7	9	theme	Sensitivity	1051:1061	arg1	analysis					1063:1070	Sensitivity analysis	1051:1070	Sensitivity analysis	1051:1070	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	3	10	theme	parameters	574:583	arg1	influence					545:553	the influence	541:553	the influence of the geometrical parameters of mold on scaffold pore structure	541:618	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	4	11	from	molds	718:722	arg1	cast					691:694	cast	691:694	cast	691:694	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	9	12	theme	validated	1343:1351	arg1	model					1353:1357	This validated model	1338:1357	This validated model	1338:1357	This validated model demonstrates a method for optimizing the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications.
31654152	0	13	theme	Tissue	84:89	arg1	Engineering					91:101	Tissue Engineering	84:101	Tissue Engineering	84:101	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	6	14	theme	APS	969:971	arg1	measurements					973:984	APS measurements	969:984	APS measurements from a corresponding experimental dataset	969:1026	The predicted APS compared favorably with APS measurements from a corresponding experimental dataset, validating the model.
31654152	3	15	from	influence	545:553	arg1	structure					610:618	scaffold pore structure	596:618	scaffold pore structure	596:618	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	16	theme	Computational	378:390	arg1	Dynamics					398:405	Computational Fluid Dynamics	378:405	Computational Fluid Dynamics	378:405	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	0	17	from	Size	32:35	arg1	Chitosan-Alginate					52:68	Freeze-Cast Chitosan-Alginate	40:68	Freeze-Cast Chitosan-Alginate	40:68	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	1	18	theme	porous	184:189	arg1	structures					191:200	highly porous structures	177:200	highly porous structures	177:200	Freeze-casting is a popular method to produce biomaterial scaffolds with highly porous structures.
31654152	3	19	theme	Fluid	392:396	arg1	Dynamics					398:405	Computational Fluid Dynamics	378:405	Computational Fluid Dynamics	378:405	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	7	20	theme	well	1169:1172	arg1	radius					1174:1179	the mold: well radius	1159:1179	the mold: well radius	1159:1179	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	3	21	theme	geometrical	562:572	arg1	parameters					574:583	the geometrical parameters	558:583	the geometrical parameters of mold	558:591	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	5	22	theme	solidification	820:833	arg1	pattern					835:841	solidification pattern	820:841	solidification pattern	820:841	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	3	23	theme	scaffold	596:603	arg1	structure					610:618	scaffold pore structure	596:618	scaffold pore structure	596:618	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	4	24	from	cast	691:694	arg1	molds					718:722	different diameter molds	699:722	different diameter molds	699:722	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	3	25	theme	pore	470:473	arg1	APS					481:483	APS	481:483	APS	481:483	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	25	theme	pore	470:473	arg1	size					475:478	average pore size	462:478	average pore size (APS) of 3D porous chitosan-alginate scaffolds	462:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	8	26	theme	pore	1235:1238	arg1	size					1240:1243	The pore size	1231:1243	The pore size	1231:1243	The pore size was most sensitive to the distance between the wells and least sensitive to solution height.
31654152	8	26	theme	pore	1235:1238	arg1	sensitive					1254:1262	sensitive	1254:1262	sensitive	1254:1262	The pore size was most sensitive to the distance between the wells and least sensitive to solution height.
31654152	7	27	theme	spacing	1208:1214	arg1	parameters					1145:1154	the three geometrical parameters	1123:1154	the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells	1123:1228	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	0	28	theme	Geometry	15:22	arg1	Effect					0:5	Effect	0:5	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate	0:68	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	3	29	theme	pore	605:608	arg1	structure					610:618	scaffold pore structure	596:618	scaffold pore structure	596:618	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	4	30	theme	different	699:707	arg1	molds					718:722	different diameter molds	699:722	different diameter molds	699:722	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	3	31	theme	Population	412:421	arg1	Model					431:435	Population Balance Model	412:435	Population Balance Model	412:435	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	32	theme	mold	588:591	arg1	parameters					574:583	the geometrical parameters	558:583	the geometrical parameters of mold	558:591	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	4	33	dep	scaffolds	681:689	arg1	filled					728:733	filled	728:733	filled to different heights	728:754	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	4	33	dep	scaffolds	681:689	arg1	cast					691:694	cast	691:694	cast	691:694	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	3	34	theme	3D	489:490	arg1	scaffolds					517:525	3D porous chitosan-alginate scaffolds	489:525	3D porous chitosan-alginate scaffolds	489:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	0	35	theme	Mold	10:13	arg1	Geometry					15:22	Mold Geometry	10:22	Mold Geometry	10:22	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	3	36	theme	porous	492:497	arg1	scaffolds					517:525	3D porous chitosan-alginate scaffolds	489:525	3D porous chitosan-alginate scaffolds	489:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	0	37	theme	Pore	27:30	arg1	Size					32:35	Pore Size	27:35	Pore Size in Freeze-Cast Chitosan-Alginate	27:68	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	4	38	theme	diameter	709:716	arg1	molds					718:722	different diameter molds	699:722	different diameter molds	699:722	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	6	39	theme	predicted	931:939	arg1	APS					941:943	The predicted APS	927:943	The predicted APS	927:943	The predicted APS compared favorably with APS measurements from a corresponding experimental dataset, validating the model.
31654152	2	40	theme	scaffolds	249:257	arg1	structure					212:220	The pore structure	203:220	The pore structure of freeze-cast biomaterial scaffolds	203:257	The pore structure of freeze-cast biomaterial scaffolds is influenced by processing parameters but has mostly been controlled experimentally.
31654152	5	41	theme	APS	910:912	arg1	homogeneity					914:924	APS homogeneity	910:924	APS homogeneity	910:924	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	3	42	theme	average	462:468	arg1	APS					481:483	APS	481:483	APS	481:483	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	42	theme	average	462:468	arg1	size					475:478	average pore size	462:478	average pore size (APS) of 3D porous chitosan-alginate scaffolds	462:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	5	43	dep	gradient	807:814	arg1	the					791:793	the	791:793	the	791:793	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	2	44	theme	biomaterial	237:247	arg1	scaffolds					249:257	freeze-cast biomaterial scaffolds	225:257	freeze-cast biomaterial scaffolds	225:257	The pore structure of freeze-cast biomaterial scaffolds is influenced by processing parameters but has mostly been controlled experimentally.
31654152	9	45	theme	scaffolds	1431:1439	arg1	APS					1400:1402	the APS	1396:1402	the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications	1396:1499	This validated model demonstrates a method for optimizing the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications.
31654152	5	46	theme	ice	850:852	arg1	nucleation					862:871	ice crystal nucleation	850:871	ice crystal nucleation	850:871	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	9	47	theme	freeze-cast	1407:1417	arg1	scaffolds					1431:1439	freeze-cast biomaterial scaffolds	1407:1439	freeze-cast biomaterial scaffolds that could be applied to other compositions or applications	1407:1499	This validated model demonstrates a method for optimizing the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications.
31654152	6	48	from	dataset	1020:1026	arg1	measurements					973:984	APS measurements	969:984	APS measurements from a corresponding experimental dataset	969:1026	The predicted APS compared favorably with APS measurements from a corresponding experimental dataset, validating the model.
31654152	2	49	theme	freeze-cast	225:235	arg1	scaffolds					249:257	freeze-cast biomaterial scaffolds	225:257	freeze-cast biomaterial scaffolds	225:257	The pore structure of freeze-cast biomaterial scaffolds is influenced by processing parameters but has mostly been controlled experimentally.
31654152	7	50	theme	geometrical	1133:1143	arg1	parameters					1145:1154	the three geometrical parameters	1123:1154	the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells	1123:1228	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	5	51	theme	crystal	854:860	arg1	nucleation					862:871	ice crystal nucleation	850:871	ice crystal nucleation	850:871	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	9	52	theme	biomaterial	1419:1429	arg1	scaffolds					1431:1439	freeze-cast biomaterial scaffolds	1407:1439	freeze-cast biomaterial scaffolds that could be applied to other compositions or applications	1407:1499	This validated model demonstrates a method for optimizing the APS of freeze-cast biomaterial scaffolds that could be applied to other compositions or applications.
31654152	7	53	theme	fill	1191:1194	arg1	height					1196:1201	solution fill height	1182:1201	solution fill height	1182:1201	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	4	54	theme	different	738:746	arg1	heights					748:754	different heights	738:754	different heights	738:754	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
31654152	6	55	theme	experimental	1007:1018	arg1	dataset					1020:1026	a corresponding experimental dataset	991:1026	a corresponding experimental dataset	991:1026	The predicted APS compared favorably with APS measurements from a corresponding experimental dataset, validating the model.
31654152	2	56	theme	pore	207:210	arg1	structure					212:220	The pore structure	203:220	The pore structure of freeze-cast biomaterial scaffolds	203:257	The pore structure of freeze-cast biomaterial scaffolds is influenced by processing parameters but has mostly been controlled experimentally.
31654152	2	57	theme	processing	276:285	arg1	parameters					287:296	processing parameters	276:296	processing parameters	276:296	The pore structure of freeze-cast biomaterial scaffolds is influenced by processing parameters but has mostly been controlled experimentally.
31654152	1	58	theme	popular	124:130	arg1	method					132:137	a popular method	122:137	a popular method to produce biomaterial scaffolds with highly porous structures	122:200	Freeze-casting is a popular method to produce biomaterial scaffolds with highly porous structures.
31654152	1	58	theme	popular	124:130	arg1	Freeze-casting					104:117	Freeze-casting	104:117	Freeze-casting	104:117	Freeze-casting is a popular method to produce biomaterial scaffolds with highly porous structures.
31654152	7	59	theme	APS	1116:1118	arg1	response					1100:1107	the response	1096:1107	the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells	1096:1228	Sensitivity analysis was performed to assess the response of the APS to the three geometrical parameters of the mold: well radius; solution fill height; and spacing between wells.
31654152	3	60	theme	scaffolds	517:525	arg1	APS					481:483	APS	481:483	APS	481:483	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	60	theme	scaffolds	517:525	arg1	size					475:478	average pore size	462:478	average pore size (APS) of 3D porous chitosan-alginate scaffolds	462:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	3	61	theme	chitosan-alginate	499:515	arg1	scaffolds					517:525	3D porous chitosan-alginate scaffolds	489:525	3D porous chitosan-alginate scaffolds	489:525	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	5	62	theme	temperature	795:805	arg1	gradient					807:814	temperature gradient	795:814	temperature gradient	795:814	The predictions demonstrated that the temperature gradient and solidification pattern affect ice crystal nucleation and growth, subsequently influencing APS homogeneity.
31654152	0	63	theme	Freeze-Cast	40:50	arg1	Chitosan-Alginate					52:68	Freeze-Cast Chitosan-Alginate	40:68	Freeze-Cast Chitosan-Alginate	40:68	Effect of Mold Geometry on Pore Size in Freeze-Cast Chitosan-Alginate Scaffolds for Tissue Engineering.
31654152	6	64	theme	corresponding	993:1005	arg1	dataset					1020:1026	a corresponding experimental dataset	991:1026	a corresponding experimental dataset	991:1026	The predicted APS compared favorably with APS measurements from a corresponding experimental dataset, validating the model.
31654152	3	65	theme	Balance	423:429	arg1	Model					431:435	Population Balance Model	412:435	Population Balance Model	412:435	A mathematical model integrating Computational Fluid Dynamics with Population Balance Model was developed to predict average pore size (APS) of 3D porous chitosan-alginate scaffolds and to assess the influence of the geometrical parameters of mold on scaffold pore structure.
31654152	4	66	theme	crystallization	645:659	arg1	pattern					661:667	the crystallization pattern	641:667	the crystallization pattern	641:667	The model predicted the crystallization pattern and APS for scaffolds cast in different diameter molds and filled to different heights.
30241812	0	0	theme	peritoneal	146:155	arg1	prevention					166:175	postsurgical peritoneal adhesion prevention	133:175	postsurgical peritoneal adhesion prevention	133:175	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	6	1	theme	25/25/50	1033:1040	arg1	proportion					1019:1028	the proportion	1015:1028	the proportion of 25/25/50	1015:1040	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	7	2	theme	anti-adhesion	1251:1263	arg1	membrane					1265:1272	the anti-adhesion membrane	1247:1272	the anti-adhesion membrane	1247:1272	The results indicated that the anti-adhesion membrane can significantly prevent peritoneal adhesion with negligible immunogenicity.
30241812	6	3	theme	anti-adhesion	987:999	arg1	membrane					1001:1008	the anti-adhesion membrane	983:1008	the anti-adhesion membrane with the proportion of 25/25/50	983:1040	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	1	4	theme	pelvic	234:239	arg1	surgery					255:261	pelvic and abdominal surgery	234:261	surgery	255:261	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	0	5	theme	postsurgical	133:144	arg1	prevention					166:175	postsurgical peritoneal adhesion prevention	133:175	postsurgical peritoneal adhesion prevention	133:175	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	3	6	link	crosslinked	519:529	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	0	7	link	crosslinked	42:52	arg1	chitosan/carboxymethyl					68:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	2	8	theme	physical	418:425	arg1	barriers					427:434	physical barriers	418:434	physical barriers	418:434	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	2	8	theme	physical	418:425	arg1	membranes					385:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	3	9	with	membrane	627:634	arg1	proportions					649:659	various proportions	641:659	various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	641:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	3	10	theme	CMCL	670:673	arg1	proportions					649:659	various proportions	641:659	various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	641:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	1	11	theme	organ	325:329	arg1	injury					331:336	organ injury	325:336	organ injury	325:336	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	0	12	theme	adhesion	157:164	arg1	prevention					166:175	postsurgical peritoneal adhesion prevention	133:175	postsurgical peritoneal adhesion prevention	133:175	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	3	13	theme	CMCS	664:667	arg1	proportions					649:659	various proportions	641:659	various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	641:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	4	14	theme	mechanical	812:821	arg1	properties					823:832	mechanical properties	812:832	mechanical properties	812:832	After crosslinking by TGase, the composite anti-adhesion membranes shown enhanced mechanical properties and improved biodegradability.
30241812	4	15	theme	anti-adhesion	773:785	arg1	membranes					787:795	the composite anti-adhesion membranes	759:795	the composite anti-adhesion membranes shown	759:801	After crosslinking by TGase, the composite anti-adhesion membranes shown enhanced mechanical properties and improved biodegradability.
30241812	8	16	theme	low	1477:1479	arg1	antigenicity					1481:1492	low antigenicity	1477:1492	low antigenicity	1477:1492	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	5	17	theme	cell	953:956	arg1	study					966:970	in vitro cell culture study	944:970	in vitro cell culture study	944:970	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	3	18	theme	various	641:647	arg1	proportions					649:659	various proportions	641:659	various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	641:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	8	19	theme	anti-adhesive	1424:1436	arg1	effects					1438:1444	satisfactory anti-adhesive effects	1411:1444	satisfactory anti-adhesive effects	1411:1444	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	1	20	theme	abdominal	245:253	arg1	surgery					255:261	pelvic and abdominal surgery	234:261	surgery	255:261	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	6	21	dep	observation	1149:1159	arg1	assay					1213:1217	assay	1213:1217	assay	1213:1217	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	8	22	theme	preventive	1520:1529	arg1	barrier					1531:1537	a preventive barrier	1518:1537	a preventive barrier for peritoneal adhesion	1518:1561	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	8	22	theme	preventive	1520:1529	arg1	which					1495:1499	which	1495:1499	which	1495:1499	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	7	23	theme	negligible	1325:1334	arg1	immunogenicity					1336:1349	negligible immunogenicity	1325:1349	negligible immunogenicity	1325:1349	The results indicated that the anti-adhesion membrane can significantly prevent peritoneal adhesion with negligible immunogenicity.
30241812	5	24	theme	high	880:883	arg1	cytocompatibility					885:901	the high cytocompatibility	876:901	the high cytocompatibility of anti-adhesion membranes	876:928	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	5	24	theme	high	880:883	arg1	Meanwhile					865:873	Meanwhile	865:873	Meanwhile	865:873	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	8	25	theme	peritoneal	1543:1552	arg1	adhesion					1554:1561	peritoneal adhesion	1543:1561	peritoneal adhesion	1543:1561	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	6	26	theme	inflammatory	1192:1203	arg1	factors					1205:1211	inflammatory factors	1192:1211	inflammatory factors	1192:1211	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	0	27	theme	Transglutaminase-catalyzed	0:25	arg1	preparation					27:37	Transglutaminase-catalyzed preparation	0:37	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl	0:89	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	6	28	theme	peritoneal	1100:1109	arg1	wall					1111:1114	peritoneal wall	1100:1114	peritoneal wall	1100:1114	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	8	29	contain	had	1407:1409	arg1	membrane					1377:1384	the composite membrane	1363:1384	the composite membrane crosslinked by TGase	1363:1405	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	8	29	contain	had	1407:1409	arg2	effects					1438:1444	satisfactory anti-adhesive effects	1411:1444	satisfactory anti-adhesive effects	1411:1444	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	2	30	theme	polymer	377:383	arg1	barriers					427:434	physical barriers	418:434	physical barriers	418:434	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	2	30	theme	polymer	377:383	arg1	membranes					385:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	2	31	theme	peritoneum	447:456	arg1	adhesion					458:465	peritoneum adhesion	447:465	peritoneum adhesion	447:465	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	0	32	theme	crosslinked	42:52	arg1	chitosan/carboxymethyl					68:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	6	33	with	membrane	1001:1008	arg1	proportion					1019:1028	the proportion	1015:1028	the proportion of 25/25/50	1015:1040	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	2	34	theme	Biodegradable	363:375	arg1	barriers					427:434	physical barriers	418:434	physical barriers	418:434	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	2	34	theme	Biodegradable	363:375	arg1	membranes					385:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes	363:393	Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion.
30241812	3	35	theme	carboxymethyl	531:543	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	5	36	theme	in	944:945	arg1	study					966:970	in vitro cell culture study	944:970	in vitro cell culture study	944:970	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	1	37	theme	female	343:348	arg1	infertility					350:360	female infertility	343:360	female infertility	343:360	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	1	38	theme	Peritoneal	178:187	arg1	adhesion					189:196	Peritoneal adhesion	178:196	Peritoneal adhesion	178:196	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	1	38	theme	Peritoneal	178:187	arg1	complication					211:222	a general complication	201:222	a general complication	201:222	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	3	39	theme	chitosan/carboxymethyl	545:566	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	0	40	theme	chitosan/carboxymethyl	68:89	arg1	preparation					27:37	Transglutaminase-catalyzed preparation	0:37	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl	0:89	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	6	41	theme	defected	1081:1088	arg1	cecum					1090:1094	the artificially defected cecum	1064:1094	the artificially defected cecum	1064:1094	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	5	42	theme	culture	958:964	arg1	study					966:970	in vitro cell culture study	944:970	in vitro cell culture study	944:970	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	8	43	theme	satisfactory	1411:1422	arg1	effects					1438:1444	satisfactory anti-adhesive effects	1411:1444	satisfactory anti-adhesive effects	1411:1444	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	3	44	theme	-catalyzed	508:517	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	0	45	theme	carboxymethyl	54:66	arg1	chitosan/carboxymethyl					68:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	crosslinked carboxymethyl chitosan/carboxymethyl	42:89	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	7	46	theme	peritoneal	1300:1309	arg1	adhesion					1311:1318	peritoneal adhesion	1300:1318	peritoneal adhesion	1300:1318	The results indicated that the anti-adhesion membrane can significantly prevent peritoneal adhesion with negligible immunogenicity.
30241812	1	47	theme	chronic	282:288	arg1	pain					300:303	chronic abdominal pain	282:303	chronic abdominal pain	282:303	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	0	48	theme	composite	110:118	arg1	membrane					120:127	composite membrane	110:127	composite membrane for postsurgical peritoneal adhesion prevention	110:175	Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention.
30241812	3	49	theme	composite	603:611	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	8	50	theme	composite	1367:1375	arg1	membrane					1377:1384	the composite membrane	1363:1384	the composite membrane crosslinked by TGase	1363:1405	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	3	51	theme	COL	680:682	arg1	proportions					649:659	various proportions	641:659	various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	641:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	3	52	theme	CMCS/CMCL/COL	588:600	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	5	53	theme	membranes	920:928	arg1	cytocompatibility					885:901	the high cytocompatibility	876:901	the high cytocompatibility of anti-adhesion membranes	876:928	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	5	53	theme	membranes	920:928	arg1	Meanwhile					865:873	Meanwhile	865:873	Meanwhile	865:873	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	5	54	dep	in	944:945	arg1	vitro					947:951	vitro	947:951	vitro	947:951	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	3	55	theme	anti-adhesion	613:625	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	3	56	theme	cellulose/collagen	568:585	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	8	57	dep	had	1407:1409	arg1	used					1510:1513	used	1510:1513	could be used as a preventive barrier for peritoneal adhesion	1501:1561	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	1	58	theme	abdominal	290:298	arg1	pain					300:303	chronic abdominal pain	282:303	chronic abdominal pain	282:303	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	8	59	used	used	1510:1513	arg2	barrier					1531:1537	a preventive barrier	1518:1537	a preventive barrier for peritoneal adhesion	1518:1561	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	8	59	used	used	1510:1513	arg2	which					1495:1499	which	1495:1499	which	1495:1499	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	1	60	theme	general	203:209	arg1	adhesion					189:196	Peritoneal adhesion	178:196	Peritoneal adhesion	178:196	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	1	60	theme	general	203:209	arg1	complication					211:222	a general complication	201:222	a general complication	201:222	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
30241812	6	61	theme	general	1141:1147	arg1	observation					1149:1159	general observation	1141:1159	general observation	1141:1159	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	3	62	theme	crosslinked	519:529	arg1	membrane					627:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane	482:634	a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50)	482:713	In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed.
30241812	8	63	theme	high	1451:1454	arg1	biocompatibility					1456:1471	high biocompatibility	1451:1471	high biocompatibility	1451:1471	Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.
30241812	4	64	theme	composite	763:771	arg1	membranes					787:795	the composite anti-adhesion membranes	759:795	the composite anti-adhesion membranes shown	759:801	After crosslinking by TGase, the composite anti-adhesion membranes shown enhanced mechanical properties and improved biodegradability.
30241812	5	65	theme	anti-adhesion	906:918	arg1	membranes					920:928	anti-adhesion membranes	906:928	anti-adhesion membranes	906:928	Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study.
30241812	6	66	theme	histological	1162:1173	arg1	examination					1175:1185	histological examination	1162:1185	histological examination	1162:1185	Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay.
30241812	1	67	theme	bowel	306:310	arg1	obstruction					312:322	bowel obstruction	306:322	bowel obstruction	306:322	Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility.
29595164	0	0	theme	zero-valent	89:99	arg1	iron					101:104	CMC-stabilized nanoscale zero-valent iron	64:104	CMC-stabilized nanoscale zero-valent iron composited with biochar	64:128	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	2	1	theme	chromium	490:497	arg1	soil					521:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	2	theme	Crtotal	923:929	arg1	leachability					896:907	the leachability	892:907	the leachability of Cr(VI) and Crtotal	892:929	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	1	3	theme	remediation	150:160	arg1	experiments					162:172	the remediation experiments	146:172	the remediation experiments	146:172	In this study, the remediation experiments were performed outdoors in natural conditions.
29595164	3	4	theme	Cr	819:820	arg1	soil					835:838	Cr contaminated soil	819:838	Cr contaminated soil	819:838	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	0	5	theme	nanoscale	79:87	arg1	iron					101:104	CMC-stabilized nanoscale zero-valent iron	64:104	CMC-stabilized nanoscale zero-valent iron composited with biochar	64:128	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	3	6	theme	leaching	673:680	arg1	test					699:702	toxicity characteristic leaching procedure (TCLP) test	649:702	toxicity characteristic leaching procedure (TCLP) test	649:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	7	dep	in	456:457	arg1	situ					459:462	situ	459:462	situ	459:462	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	4	8	theme	sequential	990:999	arg1	procedure					1012:1020	sequential extraction procedure	990:1020	sequential extraction procedure (SEP)	990:1026	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	4	8	theme	sequential	990:999	arg1	SEP					1023:1025	SEP	1023:1025	SEP	1023:1025	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	3	9	theme	contaminated	822:833	arg1	soil					835:838	Cr contaminated soil	819:838	Cr contaminated soil	819:838	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	10	theme	contaminated	508:519	arg1	soil					521:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	11	theme	procedure	682:690	arg1	test					699:702	toxicity characteristic leaching procedure (TCLP) test	649:702	toxicity characteristic leaching procedure (TCLP) test	649:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	12	dep	soil	521:524	arg1	VI					503:504	VI	503:504	VI	503:504	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	13	theme	test	699:702	arg1	results					638:644	The results	634:644	The results of toxicity characteristic leaching procedure (TCLP) test	634:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	3	14	dep	Cr	912:913	arg1	VI					915:916	VI	915:916	VI	915:916	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	15	theme	untreated	618:626	arg1	soil					628:631	the untreated soil	614:631	the untreated soil	614:631	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	4	16	theme	exchangeable	1045:1056	arg1	Cr					1058:1059	most exchangeable Cr	1040:1059	most exchangeable Cr	1040:1059	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	2	17	theme	hexavalent	479:488	arg1	chromium					490:497	hexavalent chromium	479:497	hexavalent chromium [Cr(VI)] contaminated soil	479:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	4	18	dep	carbonate-bound	1074:1088	arg1	oxides-bound					1100:1111	oxides-bound	1100:1111	oxides-bound	1100:1111	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	0	19	theme	In	0:1	arg1	remediation					8:18	In situ remediation	0:18	In situ remediation of hexavalent chromium	0:41	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	4	20	theme	Cr	1160:1161	arg1	leachability					1144:1155	leachability	1144:1155	leachability	1144:1155	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	4	20	theme	Cr	1160:1161	arg1	availability					1127:1138	availability	1127:1138	availability	1127:1138	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	3	21	theme	toxicity	649:656	arg1	test					699:702	toxicity characteristic leaching procedure (TCLP) test	649:702	toxicity characteristic leaching procedure (TCLP) test	649:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	4	22	dep	availability	1127:1138	arg1	the					1123:1125	the	1123:1125	the	1123:1125	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	4	23	from	leachability	1144:1155	arg1	soil					1170:1173	the soil	1166:1173	the soil	1166:1173	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	1	24	theme	natural	201:207	arg1	conditions					209:218	natural conditions	201:218	natural conditions	201:218	In this study, the remediation experiments were performed outdoors in natural conditions.
29595164	0	25	theme	chromium	34:41	arg1	remediation					8:18	In situ remediation	0:18	In situ remediation of hexavalent chromium	0:41	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	4	26	from	availability	1127:1138	arg1	soil					1170:1173	the soil	1166:1173	the soil	1166:1173	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	0	27	theme	hexavalent	23:32	arg1	chromium					34:41	hexavalent chromium	23:41	hexavalent chromium	23:41	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	2	28	theme	soil	521:524	arg1	remediation					464:474	the in situ remediation	452:474	the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil	452:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	29	theme	CMC	246:248	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	29	theme	CMC	246:248	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	30	theme	remediation	749:759	arg1	materials					761:769	remediation materials	749:769	remediation materials	749:769	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	31	theme	zero-valent	343:353	arg1	iron					355:358	CMC-stabilized nanoscale zero-valent iron	318:358	CMC-stabilized nanoscale zero-valent iron	318:358	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	32	theme	in	456:457	arg1	remediation					464:474	the in situ remediation	452:474	the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil	452:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	33	theme	iron	561:564	arg1	concentration					534:546	the concentration	530:546	the concentration of available iron	530:564	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	34	theme	Carboxymethyl	221:233	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	34	theme	Carboxymethyl	221:233	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	35	theme	cellulose	235:243	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	35	theme	cellulose	235:243	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	36	theme	Cr	912:913	arg1	leachability					896:907	the leachability	892:907	the leachability of Cr(VI) and Crtotal	892:929	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	37	theme	nanoscale	333:341	arg1	iron					355:358	CMC-stabilized nanoscale zero-valent iron	318:358	CMC-stabilized nanoscale zero-valent iron	318:358	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	0	38	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	2	39	theme	available	551:559	arg1	iron					561:564	available iron	551:564	available iron	551:564	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	40	theme	characteristic	658:671	arg1	test					699:702	toxicity characteristic leaching procedure (TCLP) test	649:702	toxicity characteristic leaching procedure (TCLP) test	649:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	2	41	theme	CMC-stabilized	318:331	arg1	iron					355:358	CMC-stabilized nanoscale zero-valent iron	318:358	CMC-stabilized nanoscale zero-valent iron	318:358	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	4	42	theme	extraction	1001:1010	arg1	procedure					1012:1020	sequential extraction procedure	990:1020	sequential extraction procedure (SEP)	990:1026	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	4	42	theme	extraction	1001:1010	arg1	SEP					1023:1025	SEP	1023:1025	SEP	1023:1025	Moreover, sequential extraction procedure (SEP) showed that most exchangeable Cr converted to carbonate-bound and Fe-Mn oxides-bound, reducing the availability and leachability of Cr in the soil.
29595164	2	43	theme	zero-valent	272:282	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	43	theme	zero-valent	272:282	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	44	theme	soil	835:838	arg1	rate					811:814	the remediation rate	795:814	the remediation rate of Cr contaminated soil	795:838	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	3	45	theme	remediation	799:809	arg1	rate					811:814	the remediation rate	795:814	the remediation rate of Cr contaminated soil	795:838	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	0	46	theme	CMC-stabilized	64:77	arg1	iron					101:104	CMC-stabilized nanoscale zero-valent iron	64:104	CMC-stabilized nanoscale zero-valent iron composited with biochar	64:128	In situ remediation of hexavalent chromium contaminated soil by CMC-stabilized nanoscale zero-valent iron composited with biochar.
29595164	2	47	theme	nanoscale	262:270	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	47	theme	nanoscale	262:270	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	48	theme	[Cr	499:501	arg1	soil					521:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	hexavalent chromium [Cr(VI)] contaminated soil	479:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	49	from	effect	442:447	arg1	remediation					464:474	the in situ remediation	452:474	the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil	452:524	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	50	theme	-stabilized	250:260	arg1	CMC-nZVI					290:297	CMC-nZVI	290:297	CMC-nZVI	290:297	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	2	50	theme	-stabilized	250:260	arg1	iron					284:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron	221:287	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI)	221:298	Carboxymethyl cellulose (CMC)-stabilized nanoscale zero-valent iron (CMC-nZVI), biochar (BC) and CMC-stabilized nanoscale zero-valent iron composited with biochar (CMC-nZVI/BC) were synthesized and investigated for their effect on the in situ remediation of hexavalent chromium [Cr(VI)] contaminated soil and the concentration of available iron was tested after the remediation, compared with the untreated soil.
29595164	3	51	theme	TCLP	693:696	arg1	test					699:702	toxicity characteristic leaching procedure (TCLP) test	649:702	toxicity characteristic leaching procedure (TCLP) test	649:702	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	3	52	theme	CMC-nZVI	862:869	arg1	g/Kg					886:889	2.5 g/Kg	882:889	2.5 g/Kg	882:889	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
29595164	3	52	theme	CMC-nZVI	862:869	arg1	ratio					853:857	the ratio	849:857	the ratio of CMC-nZVI to Fe0	849:876	The results of toxicity characteristic leaching procedure (TCLP) test showed that CMC-nZVI and CMC-nZVI/BC used as remediation materials could obviously improve the remediation rate of Cr contaminated soil and when the ratio of CMC-nZVI to Fe0 was 2.5 g/Kg, the leachability of Cr(VI) and Crtotal can be reduced by 100% and 95.8% simultaneously.
31577976	10	0	from	adherence	1129:1137	arg1	mucosa					1167:1172	intestinal mucosa	1156:1172	intestinal mucosa	1156:1172	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	1	with	HNTs/micro-composites	1050:1070	arg1	concentration					1085:1097	highest concentration	1077:1097	highest concentration of chitosan	1077:1109	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	1	2	theme	halloysite	164:173	arg1	HNTs					186:189	HNTs	186:189	HNTs	186:189	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	1	2	theme	halloysite	164:173	arg1	nanotubes					175:183	halloysite nanotubes	164:183	halloysite nanotubes (HNTs)	164:190	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	8	3	theme	rough	878:882	arg1	surface					898:904	rough curved porous surface	878:904	rough curved porous surface	878:904	SEM showed a spherical shape with rough curved porous surface.
31577976	1	4	theme	drug	260:263	arg1	system					274:279	mucoadhesive sustained release drug delivery system	229:279	mucoadhesive sustained release drug delivery system	229:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	9	5	theme	XRD	991:993	arg1	results					995:1001	XRD results	991:1001	XRD results	991:1001	Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites.
31577976	4	6	theme	surface	534:540	arg1	morphology					542:551	their surface morphology	528:551	their surface morphology	528:551	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	5	7	theme	model	690:694	arg1	drug					696:699	model drug	690:699	model drug	690:699	Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug.
31577976	2	8	theme	swelling	375:382	arg1	behavior					384:391	swelling behavior	375:391	swelling behavior	375:391	Micro-composites were characterized for preparation yield, size, micromeritic properties and swelling behavior.
31577976	10	9	theme	coated	1269:1274	arg1	HNTs					1276:1279	coated HNTs	1269:1279	coated HNTs	1269:1279	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	1	10	theme	delivery	265:272	arg1	system					274:279	mucoadhesive sustained release drug delivery system	229:279	mucoadhesive sustained release drug delivery system	229:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	10	11	theme	intestinal	1156:1165	arg1	mucosa					1167:1172	intestinal mucosa	1156:1172	intestinal mucosa	1156:1172	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	12	from	pH	1220:1221	arg1	%					1215:1215	66.8%	1211:1215	66.8% at pH 1.2	1211:1225	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	12	from	pH	1220:1221	arg1	%					1235:1235	46.7%	1231:1235	46.7% at pH 5.5	1231:1245	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	8	13	theme	porous	891:896	arg1	surface					898:904	rough curved porous surface	878:904	rough curved porous surface	878:904	SEM showed a spherical shape with rough curved porous surface.
31577976	3	14	theme	Chemical	394:401	arg1	composition					403:413	Chemical composition	394:413	Chemical composition of micro-composites	394:433	Chemical composition of micro-composites was characterized by FTIR, XRD and TGA.
31577976	5	15	theme	release	633:639	arg1	behavior					641:648	release behavior	633:648	release behavior of metoclopramide hydrochloride used as model drug	633:699	Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug.
31577976	10	16	theme	25 h.	1254:1258	arg1	Chitosan					1260:1267	25 h. Chitosan	1254:1267	25 h. Chitosan	1254:1267	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	9	17	theme	crystalline	1010:1020	arg1	nature					1022:1027	crystalline nature	1010:1027	crystalline nature of micro-composites	1010:1047	Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites.
31577976	5	18	theme	metoclopramide	653:666	arg1	hydrochloride					668:680	metoclopramide hydrochloride	653:680	metoclopramide hydrochloride used as model drug	653:699	Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug.
31577976	5	19	theme	intestinal	600:609	arg1	mucosa					611:616	intestinal mucosa	600:616	intestinal mucosa	600:616	Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug.
31577976	9	20	theme	micro-composites	1032:1047	arg1	nature					1022:1027	crystalline nature	1010:1027	crystalline nature of micro-composites	1010:1047	Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites.
31577976	3	21	theme	micro-composites	418:433	arg1	composition					403:413	Chemical composition	394:413	Chemical composition of micro-composites	394:433	Chemical composition of micro-composites was characterized by FTIR, XRD and TGA.
31577976	10	22	theme	Drug	1185:1188	arg1	release					1190:1196	3 h. Drug release	1180:1196	3 h. Drug release recorded	1180:1205	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	5	23	theme	hydrochloride	668:680	arg1	behavior					641:648	release behavior	633:648	release behavior of metoclopramide hydrochloride used as model drug	633:699	Micro-composites were studied for adhesion on intestinal mucosa as well as for release behavior of metoclopramide hydrochloride used as model drug.
31577976	10	24	dep	showed	1281:1286	arg1	proving					1352:1358	proving	1352:1358	showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system	1281:1413	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	0	25	theme	Mucoadhesive	0:11	arg1	micro-composites					13:28	Mucoadhesive micro-composites	0:28	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.	0:95	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	26	theme	maximum	1121:1127	arg1	adherence					1129:1137	maximum adherence	1121:1137	maximum adherence of 89 ± 1.79% on intestinal mucosa	1121:1172	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	0	27	theme	Chitosan	31:38	arg1	nanotubes					58:66	Chitosan coated halloysite nanotubes	31:66	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.	0:95	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	28	theme	highest	1077:1083	arg1	concentration					1085:1097	highest concentration	1077:1097	highest concentration of chitosan	1077:1109	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	29	from	pH	1240:1241	arg1	%					1215:1215	66.8%	1211:1215	66.8% at pH 1.2	1211:1225	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	29	from	pH	1240:1241	arg1	%					1235:1235	46.7%	1231:1235	46.7% at pH 5.5	1231:1245	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	30	theme	delivery	1399:1406	arg1	system					1408:1413	mucoadhesive drug delivery system	1381:1413	mucoadhesive drug delivery system	1381:1413	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	7	31	theme	0.151 ± 0.49 μm	827:841	arg1	range					818:822	a mean size range	806:822	a mean size range of 0.151 ± 0.49 μm	806:841	Micro-composites exhibited a mean size range of 0.151 ± 0.49 μm.
31577976	4	32	theme	electron	484:491	arg1	SEM					505:507	SEM	505:507	SEM	505:507	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	4	32	theme	electron	484:491	arg1	microscopy					493:502	Scanning electron microscopy	475:502	Scanning electron microscopy (SEM)	475:508	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	2	33	theme	preparation	322:332	arg1	yield					334:338	preparation yield	322:338	preparation yield	322:338	Micro-composites were characterized for preparation yield, size, micromeritic properties and swelling behavior.
31577976	10	34	theme	drug	1394:1397	arg1	system					1408:1413	mucoadhesive drug delivery system	1381:1413	mucoadhesive drug delivery system	1381:1413	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	1	35	theme	cationic	200:207	arg1	chitosan					217:224	the cationic polymer chitosan	196:224	the cationic polymer chitosan	196:224	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	0	36	theme	halloysite	47:56	arg1	nanotubes					58:66	Chitosan coated halloysite nanotubes	31:66	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.	0:95	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	4	37	theme	Scanning	475:482	arg1	SEM					505:507	SEM	505:507	SEM	505:507	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	4	37	theme	Scanning	475:482	arg1	microscopy					493:502	Scanning electron microscopy	475:502	Scanning electron microscopy (SEM)	475:508	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	6	38	theme	Preparation	702:712	arg1	yield					714:718	Preparation yield	702:718	Preparation yield	702:718	Preparation yield was found to be in the range of 35.14 ± 1.5-53.97 ± 5.23%.
31577976	1	39	theme	polymer	209:215	arg1	chitosan					217:224	the cationic polymer chitosan	196:224	the cationic polymer chitosan	196:224	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	4	40	used	used	514:517	arg2	SEM					505:507	SEM	505:507	SEM	505:507	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	4	40	used	used	514:517	arg2	microscopy					493:502	Scanning electron microscopy	475:502	Scanning electron microscopy (SEM)	475:508	Scanning electron microscopy (SEM) was used to study their surface morphology.
31577976	0	41	theme	coated	40:45	arg1	nanotubes					58:66	Chitosan coated halloysite nanotubes	31:66	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.	0:95	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	42	theme	mucoadhesive	1381:1392	arg1	system					1408:1413	mucoadhesive drug delivery system	1381:1413	mucoadhesive drug delivery system	1381:1413	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	43	theme	chitosan	1102:1109	arg1	concentration					1085:1097	highest concentration	1077:1097	highest concentration of chitosan	1077:1109	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	9	44	theme	excellent	934:942	arg1	flowability					944:954	excellent flowability	934:954	excellent flowability	934:954	Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites.
31577976	7	45	theme	mean	808:811	arg1	range					818:822	a mean size range	806:822	a mean size range of 0.151 ± 0.49 μm	806:841	Micro-composites exhibited a mean size range of 0.151 ± 0.49 μm.
31577976	1	46	theme	study	116:120	arg1	aim					104:106	aim	104:106	aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system	104:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	8	47	theme	spherical	857:865	arg1	shape					867:871	a spherical shape	855:871	a spherical shape	855:871	SEM showed a spherical shape with rough curved porous surface.
31577976	7	48	theme	size	813:816	arg1	range					818:822	a mean size range	806:822	a mean size range of 0.151 ± 0.49 μm	806:841	Micro-composites exhibited a mean size range of 0.151 ± 0.49 μm.
31577976	9	49	theme	maximum	960:966	arg1	swelling					968:975	maximum swelling	960:975	maximum swelling	960:975	Micro-composites exhibited excellent flowability and maximum swelling at acidic pH. XRD results showed crystalline nature of micro-composites.
31577976	10	50	theme	%	1151:1151	arg1	adherence					1129:1137	maximum adherence	1121:1137	maximum adherence of 89 ± 1.79% on intestinal mucosa	1121:1172	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	2	51	theme	micromeritic	347:358	arg1	properties					360:369	micromeritic properties	347:369	micromeritic properties	347:369	Micro-composites were characterized for preparation yield, size, micromeritic properties and swelling behavior.
31577976	6	52	theme	%	776:776	arg1	range					743:747	the range	739:747	the range of 35.14 ± 1.5-53.97 ± 5.23%	739:776	Preparation yield was found to be in the range of 35.14 ± 1.5-53.97 ± 5.23%.
31577976	10	53	theme	remarkable	1288:1297	arg1	mucoadhesion					1299:1310	remarkable mucoadhesion	1288:1310	remarkable mucoadhesion	1288:1310	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	1	54	theme	mucoadhesive	229:240	arg1	release					252:258	mucoadhesive sustained release	229:258	mucoadhesive sustained release drug delivery system	229:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	0	55	theme	drug	82:85	arg1	delivery					87:94	sustained drug delivery	72:94	sustained drug delivery	72:94	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	56	theme	sustained	1316:1324	arg1	release					1326:1332	sustained release	1316:1332	sustained release of metoclopramide	1316:1350	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	0	57	dep	micro-composites	13:28	arg1	nanotubes					58:66	Chitosan coated halloysite nanotubes	31:66	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.	0:95	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	58	theme	metoclopramide	1337:1350	arg1	mucoadhesion					1299:1310	remarkable mucoadhesion	1288:1310	remarkable mucoadhesion	1288:1310	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	10	58	theme	metoclopramide	1337:1350	arg1	release					1326:1332	sustained release	1316:1332	sustained release of metoclopramide	1316:1350	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	1	59	theme	sustained	242:250	arg1	release					252:258	mucoadhesive sustained release	229:258	mucoadhesive sustained release drug delivery system	229:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
31577976	0	60	theme	sustained	72:80	arg1	delivery					87:94	sustained drug delivery	72:94	sustained drug delivery	72:94	Mucoadhesive micro-composites: Chitosan coated halloysite nanotubes for sustained drug delivery.
31577976	10	61	theme	3 h.	1180:1183	arg1	release					1190:1196	3 h. Drug release	1180:1196	3 h. Drug release recorded	1180:1205	HNTs/micro-composites with highest concentration of chitosan displayed maximum adherence of 89 ± 1.79% on intestinal mucosa after 3 h. Drug release recorded was 66.8% at pH 1.2 and 46.7% at pH 5.5 within 25 h. Chitosan coated HNTs showed remarkable mucoadhesion and sustained release of metoclopramide proving their suitability as mucoadhesive drug delivery system.
31577976	8	62	theme	curved	884:889	arg1	surface					898:904	rough curved porous surface	878:904	rough curved porous surface	878:904	SEM showed a spherical shape with rough curved porous surface.
31577976	1	63	theme	release	252:258	arg1	system					274:279	mucoadhesive sustained release drug delivery system	229:279	mucoadhesive sustained release drug delivery system	229:279	It was aim of this study to synthesize micro-composites comprising halloysite nanotubes (HNTs) and the cationic polymer chitosan as mucoadhesive sustained release drug delivery system.
30328567	3	0	from	expression	1084:1093	arg1	tissue					1136:1141	myocardial tissue	1125:1141	myocardial tissue	1125:1141	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	1	theme	glycosylated	887:898	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	1	theme	glycosylated	887:898	arg1	protein					906:912	glycosylated serum protein	887:912	glycosylated serum protein	887:912	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	2	2	theme	aortic	774:779	arg1	injury					789:794	abdominal aortic balloon injury	764:794	abdominal aortic balloon injury	764:794	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	7	3	from	decline	1934:1940	arg1	group					1959:1963	CONR low-dose group	1945:1963	CONR low-dose group (P<0.01)	1945:1972	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	7	3	from	decline	1934:1940	arg1	P<0.01					1966:1971	P<0.01	1966:1971	P<0.01	1966:1971	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	1	4	theme	Coptidis	291:298	arg1	CONR					311:314	CONR	311:314	CONR	311:314	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	4	theme	Coptidis	291:298	arg1	alkaloids					300:308	Rhizoma Coptidis alkaloids	283:308	Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	283:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	5	theme	assay	1058:1062	arg1	method					1072:1077	immunosorbent assay (ELISA) method	1044:1077	immunosorbent assay (ELISA) method	1044:1077	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	5	theme	assay	1058:1062	arg1	p-JNK					1217:1221	p-JNK	1217:1221	p-JNK	1217:1221	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	5	theme	assay	1058:1062	arg1	caspase-3					1225:1233	caspase-3	1225:1233	caspase-3	1225:1233	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	8	6	theme	model	1993:1997	arg1	group					1999:2003	the model group	1989:2003	the model group	1989:2003	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	9	7	theme	better	2466:2471	arg1	effect					2473:2478	better effect	2466:2478	better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis	2466:2546	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	1	8	theme	[control	464:471	arg1	group					473:477	6 groups [control group	455:477	6 groups [control group	455:477	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	10	9	theme	DA	2660:2661	arg1	rabbits					2663:2669	DA rabbits	2660:2669	DA rabbits	2660:2669	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	5	10	theme	bcl-2	1677:1681	arg1	protein					1683:1689	bcl-2 protein	1677:1689	bcl-2 protein	1677:1689	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	FRA					1532:1534	FRA	1532:1534	FRA	1532:1534	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	expression					1554:1563	the expression	1550:1563	the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI)	1550:1642	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	AR					1537:1538	AR	1537:1538	AR	1537:1538	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	GHB					1522:1524	GHB	1522:1524	GHB	1522:1524	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	GSP					1527:1529	GSP	1527:1529	GSP	1527:1529	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	AGEs					1541:1544	AGEs	1541:1544	AGEs	1541:1544	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	11	theme	RAGE	1579:1582	arg1	FBG					1517:1519	serum FBG	1511:1519	serum FBG	1511:1519	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	3	12	from	expression	1270:1279	arg1	tissue					1295:1300	myocardial tissue	1284:1300	myocardial tissue	1284:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	2	13	theme	DA	673:674	arg1	model					676:680	DA model	673:680	DA model	673:680	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	1	14	theme	simvastatin	599:609	arg1	group					611:615	simvastatin group	599:615	simvastatin group	599:615	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	15	from	decline	1846:1852	arg1	P<0.01					1892:1897	P<0.01	1892:1897	P<0.01	1892:1897	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	6	15	from	decline	1846:1852	arg1	groups					1884:1889	CONR high- and medium-dose groups	1857:1889	CONR high- and medium-dose groups (P<0.01)	1857:1898	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	10	16	theme	protein	2726:2732	arg1	expressions					2734:2744	bcl-2 protein expressions	2720:2744	bcl-2 protein expressions	2720:2744	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	4	17	theme	labeling	1404:1411	arg1	method					1421:1426	TdT-mediated dUTPnick-end labeling (TUNEL) method	1378:1426	TdT-mediated dUTPnick-end labeling (TUNEL) method	1378:1426	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	1	18	theme	Composition	210:220	arg1	effects					199:205	the effects	195:205	the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS	195:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	8	19	theme	serum	2024:2028	arg1	AGEs					2030:2033	serum AGEs	2024:2033	serum AGEs	2024:2033	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	9	20	theme	caspase-3	2501:2509	arg1	expression					2487:2496	the expression	2483:2496	the expression of caspase-3, bcl-2 protein, myocardium apoptosis	2483:2546	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	2	21	theme	injected	715:722	arg1	alloxan					724:730	intravenously injected alloxan	701:730	intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury	701:794	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	3	22	theme	myocardial	1125:1134	arg1	tissue					1136:1141	myocardial tissue	1125:1141	myocardial tissue	1125:1141	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	23	theme	New	402:404	arg1	rabbits					420:426	Sixty male New Zealand white rabbits	391:426	Sixty male New Zealand white rabbits	391:426	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	24	theme	Ophiopogon	225:234	arg1	polysaccharide					236:249	Ophiopogon polysaccharide	225:249	Ophiopogon polysaccharide	225:249	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	9	25	theme	bcl-2	2512:2516	arg1	protein					2518:2524	bcl-2 protein	2512:2524	bcl-2 protein	2512:2524	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	25	theme	bcl-2	2512:2516	arg1	caspase-3					2501:2509	caspase-3	2501:2509	caspase-3	2501:2509	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	0	26	theme	Atherosclerosis	146:160	arg1	Rabbit					162:167	Diabetic Atherosclerosis Rabbit	137:167	Diabetic Atherosclerosis Rabbit	137:167	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	1	27	theme	white	414:418	arg1	rabbits					420:426	Sixty male New Zealand white rabbits	391:426	Sixty male New Zealand white rabbits	391:426	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	28	from	polysaccharide	236:249	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	8	29	theme	RAGE	2050:2053	arg1	AI					2095:2096	AI	2095:2096	the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI	2006:2096	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	29	theme	RAGE	2050:2053	arg1	expression					2010:2019	the expression	2006:2019	the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI	2006:2096	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	3	30	dep	method	1072:1077	arg1	measured					1307:1314	measured	1307:1314	were measured by Western blotting	1302:1334	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	8	31	contain	had	2225:2227	arg1	CONR					2220:2223	high-dose CONR	2210:2223	high-dose CONR	2210:2223	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	31	contain	had	2225:2227	arg2	effect					2250:2255	the most significant effect	2229:2255	the most significant effect on abovementioned indices	2229:2281	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	1	32	theme	low-dose	568:575	arg1	group					577:581	CONR low-dose group	563:581	CONR low-dose group (50 mg/kg)	563:592	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	32	theme	low-dose	568:575	arg1	mg/kg					587:591	50 mg/kg	584:591	50 mg/kg	584:591	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	5	33	theme	p-JNK	1585:1589	arg1	FRA					1532:1534	FRA	1532:1534	FRA	1532:1534	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	expression					1554:1563	the expression	1550:1563	the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI)	1550:1642	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	AR					1537:1538	AR	1537:1538	AR	1537:1538	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	GHB					1522:1524	GHB	1522:1524	GHB	1522:1524	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	GSP					1527:1529	GSP	1527:1529	GSP	1527:1529	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	AGEs					1541:1544	AGEs	1541:1544	AGEs	1541:1544	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	33	theme	p-JNK	1585:1589	arg1	FBG					1517:1519	serum FBG	1511:1519	serum FBG	1511:1519	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	9	34	theme	low-dose	2397:2404	arg1	P<0.01					2413:2418	P<0.01	2413:2418	P<0.01	2413:2418	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	34	theme	low-dose	2397:2404	arg1	group					2406:2410	the low-dose group	2393:2410	the low-dose group (P<0.01)	2393:2419	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	35	theme	Middle-dose	2330:2340	arg1	CONR					2342:2345	Middle-dose CONR	2330:2345	Middle-dose CONR	2330:2345	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	8	36	theme	caspase-3	2066:2074	arg1	protein					2076:2082	caspase-3 protein	2066:2082	caspase-3 protein	2066:2082	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	3	37	theme	Western	1319:1325	arg1	blotting					1327:1334	Western blotting	1319:1334	Western blotting	1319:1334	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	8	38	theme	bcl-2	2131:2135	arg1	protein					2137:2143	bcl-2 protein	2131:2143	bcl-2 protein	2131:2143	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	5	39	theme	caspase-3	1592:1600	arg1	proteins					1602:1609	caspase-3 proteins	1592:1609	caspase-3 proteins	1592:1609	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	9	40	theme	CONR	2566:2569	arg1	P<0.01					2587:2592	P<0.01	2587:2592	P<0.01	2587:2592	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	40	theme	CONR	2566:2569	arg1	group					2580:2584	the CONR low-dose group	2562:2584	the CONR low-dose group (P<0.01)	2562:2593	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	0	41	theme	Coptidis	81:88	arg1	Composition					0:10	Composition	0:10	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids	0:98	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	8	42	theme	other	2297:2301	arg1	groups					2313:2318	other treatment groups	2297:2318	other treatment groups (P<0.01)	2297:2327	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	42	theme	other	2297:2301	arg1	P<0.01					2321:2326	P<0.01	2321:2326	P<0.01	2321:2326	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	3	43	theme	aldose	942:947	arg1	reductase					949:957	aldose reductase	942:957	aldose reductase (AR)	942:962	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	43	theme	aldose	942:947	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	43	theme	aldose	942:947	arg1	AR					960:961	AR	960:961	AR	960:961	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	8	44	theme	high-dose	2210:2218	arg1	CONR					2220:2223	high-dose CONR	2210:2223	high-dose CONR	2210:2223	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	9	45	theme	serum	2368:2372	arg1	AGEs					2374:2377	serum AGEs	2368:2377	serum AGEs	2368:2377	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	3	46	theme	N-terminal	1198:1207	arg1	kinase					1209:1214	p-Jun N-terminal kinase	1192:1214	p-Jun N-terminal kinase	1192:1214	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	47	theme	medium-dose	532:542	arg1	group					544:548	CONR medium-dose group	527:548	CONR medium-dose group (150 mg/kg)	527:560	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	47	theme	medium-dose	532:542	arg1	mg/kg					555:559	150 mg/kg	551:559	150 mg/kg	551:559	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	0	48	theme	Myocardial	113:122	arg1	Apoptosis					124:132	the Myocardial Apoptosis	109:132	the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit	109:167	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	1	49	theme	Sixty	391:395	arg1	rabbits					420:426	Sixty male New Zealand white rabbits	391:426	Sixty male New Zealand white rabbits	391:426	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	50	theme	advanced	965:972	arg1	products					988:995	advanced glycation end products	965:995	advanced glycation end products (AGEs) in serum	965:1011	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	50	theme	advanced	965:972	arg1	AGEs					998:1001	AGEs	998:1001	AGEs	998:1001	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	51	theme	fasting	827:833	arg1	glucose					841:847	fasting blood glucose	827:847	fasting blood glucose (FBG)	827:853	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	51	theme	fasting	827:833	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	51	theme	fasting	827:833	arg1	FBG					850:852	FBG	850:852	FBG	850:852	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	7	52	theme	low-dose	1950:1957	arg1	group					1959:1963	CONR low-dose group	1945:1963	CONR low-dose group (P<0.01)	1945:1972	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	7	52	theme	low-dose	1950:1957	arg1	P<0.01					1966:1971	P<0.01	1966:1971	P<0.01	1966:1971	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	5	53	theme	serum	1511:1515	arg1	FBG					1517:1519	serum FBG	1511:1519	serum FBG	1511:1519	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	6	54	theme	serum	1793:1797	arg1	FBG					1799:1801	serum FBG	1793:1801	serum FBG	1793:1801	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	5	55	theme	myocardium	1568:1577	arg1	RAGE					1579:1582	myocardium RAGE	1568:1582	myocardium RAGE	1568:1582	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	8	56	from	effect	2250:2255	arg1	indices					2275:2281	abovementioned indices	2260:2281	abovementioned indices	2260:2281	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	6	57	theme	significant	1834:1844	arg1	decline					1846:1852	a significant decline	1832:1852	a significant decline in CONR high- and medium-dose groups (P<0.01)	1832:1898	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	3	58	theme	end	984:986	arg1	products					988:995	advanced glycation end products	965:995	advanced glycation end products (AGEs) in serum	965:1011	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	58	theme	end	984:986	arg1	AGEs					998:1001	AGEs	998:1001	AGEs	998:1001	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	6	59	theme	high-	1862:1866	arg1	P<0.01					1892:1897	P<0.01	1892:1897	P<0.01	1892:1897	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	6	59	theme	high-	1862:1866	arg1	groups					1884:1889	CONR high- and medium-dose groups	1857:1889	CONR high- and medium-dose groups (P<0.01)	1857:1898	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	3	60	theme	B-cell	1236:1241	arg1	expression					1270:1279	B-cell lymphoma-2 (bcl-2) protein expression	1236:1279	B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue	1236:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	61	theme	high-dose	498:506	arg1	group					508:512	CONR high-dose group	493:512	CONR high-dose group (450 mg/kg)	493:524	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	61	theme	high-dose	498:506	arg1	mg/kg					519:523	450 mg/kg	515:523	450 mg/kg	515:523	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	0	62	theme	Total	54:58	arg1	Saponins					60:67	Total Saponins	54:67	Total Saponins	54:67	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	1	63	theme	diabetic	344:351	arg1	rabbits					374:380	diabetic atherosclerosis (DA) rabbits	344:380	diabetic atherosclerosis (DA) rabbits	344:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	5	64	theme	apoptosis	1623:1631	arg1	index					1633:1637	apoptosis index	1623:1637	apoptosis index (AI)	1623:1642	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	64	theme	apoptosis	1623:1631	arg1	AI					1640:1641	AI	1640:1641	AI	1640:1641	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	1	65	dep	polysaccharide	236:249	arg1	METHODS					382:388	METHODS	382:388	METHODS	382:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	4	66	theme	dUTPnick-end	1391:1402	arg1	TUNEL					1414:1418	TUNEL	1414:1418	TUNEL	1414:1418	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	4	66	theme	dUTPnick-end	1391:1402	arg1	labeling					1404:1411	TdT-mediated dUTPnick-end labeling	1378:1411	TdT-mediated dUTPnick-end labeling (TUNEL) method	1378:1426	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	1	67	theme	Notoginseng	252:262	arg1	saponins					270:277	Notoginseng total saponins	252:277	Notoginseng total saponins	252:277	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	68	theme	serum	900:904	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	68	theme	serum	900:904	arg1	protein					906:912	glycosylated serum protein	887:912	glycosylated serum protein	887:912	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	69	theme	random	640:645	arg1	10					655:656	10	655:656	10	655:656	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	69	theme	random	640:645	arg1	method					647:652	a completely random method	627:652	a completely random method	627:652	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	10	70	from	apoptosis	2647:2655	arg1	rabbits					2663:2669	DA rabbits	2660:2669	DA rabbits	2660:2669	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	8	71	theme	significant	2238:2248	arg1	effect					2250:2255	the most significant effect	2229:2255	the most significant effect on abovementioned indices	2229:2281	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	4	72	theme	myocardial	1341:1350	arg1	apoptosis					1352:1360	The myocardial apoptosis	1337:1360	The myocardial apoptosis	1337:1360	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	1	73	theme	saponins	270:277	arg1	Composition					210:220	Composition	210:220	Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS	210:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	9	74	theme	simvastatin	2443:2453	arg1	groups					2455:2460	middle-dose CONR and simvastatin groups	2422:2460	middle-dose CONR and simvastatin groups	2422:2460	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	2	75	theme	balloon	781:787	arg1	injury					789:794	abdominal aortic balloon injury	764:794	abdominal aortic balloon injury	764:794	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	9	76	contain	had	2462:2464	arg2	effect					2473:2478	better effect	2466:2478	better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis	2466:2546	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	76	contain	had	2462:2464	arg1	groups					2455:2460	middle-dose CONR and simvastatin groups	2422:2460	middle-dose CONR and simvastatin groups	2422:2460	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	2	77	theme	abdominal	764:772	arg1	injury					789:794	abdominal aortic balloon injury	764:794	abdominal aortic balloon injury	764:794	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	10	78	theme	bcl-2	2720:2724	arg1	expressions					2734:2744	bcl-2 protein expressions	2720:2744	bcl-2 protein expressions	2720:2744	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	8	79	theme	treatment	2303:2311	arg1	groups					2313:2318	other treatment groups	2297:2318	other treatment groups (P<0.01)	2297:2327	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	79	theme	treatment	2303:2311	arg1	P<0.01					2321:2326	P<0.01	2321:2326	P<0.01	2321:2326	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	1	80	from	apoptosis	331:339	arg1	Composition					210:220	Composition	210:220	Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS	210:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	81	theme	groups	457:462	arg1	group					473:477	6 groups [control group	455:477	6 groups [control group	455:477	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	82	theme	alkaloids	300:308	arg1	Composition					210:220	Composition	210:220	Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS	210:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	83	theme	medium-dose	1872:1882	arg1	P<0.01					1892:1897	P<0.01	1892:1897	P<0.01	1892:1897	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	6	83	theme	medium-dose	1872:1882	arg1	groups					1884:1889	CONR high- and medium-dose groups	1857:1889	CONR high- and medium-dose groups (P<0.01)	1857:1898	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	4	84	theme	apoptosis	1433:1441	arg1	AI					1450:1451	AI	1450:1451	AI	1450:1451	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	4	84	theme	apoptosis	1433:1441	arg1	index					1443:1447	apoptosis index	1433:1447	apoptosis index (AI)	1433:1452	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	3	85	theme	immunosorbent	1044:1056	arg1	ELISA					1065:1069	ELISA	1065:1069	ELISA	1065:1069	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	85	theme	immunosorbent	1044:1056	arg1	assay					1058:1062	immunosorbent assay	1044:1062	immunosorbent assay (ELISA) method	1044:1077	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	9	86	theme	myocardium	2527:2536	arg1	apoptosis					2538:2546	myocardium apoptosis	2527:2546	myocardium apoptosis	2527:2546	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	86	theme	myocardium	2527:2536	arg1	caspase-3					2501:2509	caspase-3	2501:2509	caspase-3	2501:2509	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	3	87	gly	glycosylated	887:898	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	87	gly	glycosylated	887:898	arg1	protein					906:912	glycosylated serum protein	887:912	glycosylated serum protein	887:912	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	88	theme	model	480:484	arg1	group					486:490	model group	480:490	model group	480:490	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	8	89	theme	AGEs	2030:2033	arg1	AI					2095:2096	AI	2095:2096	the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI	2006:2096	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	89	theme	AGEs	2030:2033	arg1	expression					2010:2019	the expression	2006:2019	the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI	2006:2096	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	3	90	theme	AGEs	1110:1113	arg1	receptor					1098:1105	receptor	1098:1105	receptor of AGEs (RAGE)	1098:1120	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	0	91	theme	Diabetic	137:144	arg1	Rabbit					162:167	Diabetic Atherosclerosis Rabbit	137:167	Diabetic Atherosclerosis Rabbit	137:167	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	8	92	theme	myocardium	2039:2048	arg1	RAGE					2050:2053	myocardium RAGE	2039:2053	myocardium RAGE	2039:2053	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	1	93	theme	Zealand	406:412	arg1	rabbits					420:426	Sixty male New Zealand white rabbits	391:426	Sixty male New Zealand white rabbits	391:426	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	5	94	theme	proteins	1602:1609	arg1	FRA					1532:1534	FRA	1532:1534	FRA	1532:1534	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	expression					1554:1563	the expression	1550:1563	the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI)	1550:1642	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	AR					1537:1538	AR	1537:1538	AR	1537:1538	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	GHB					1522:1524	GHB	1522:1524	GHB	1522:1524	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	GSP					1527:1529	GSP	1527:1529	GSP	1527:1529	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	AGEs					1541:1544	AGEs	1541:1544	AGEs	1541:1544	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	94	theme	proteins	1602:1609	arg1	FBG					1517:1519	serum FBG	1511:1519	serum FBG	1511:1519	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	1	95	theme	polysaccharide	236:249	arg1	Composition					210:220	Composition	210:220	Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS	210:388	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	96	theme	receptor	1098:1105	arg1	expression					1084:1093	the expression	1080:1093	the expression of receptor of AGEs (RAGE) in myocardial tissue	1080:1141	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	97	theme	myocardial	1284:1293	arg1	tissue					1295:1300	myocardial tissue	1284:1300	myocardial tissue	1284:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	98	gly	glycosylated	856:867	arg1	GHB					881:883	GHB	881:883	GHB	881:883	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	98	gly	glycosylated	856:867	arg1	hemoglobin					869:878	glycosylated hemoglobin	856:878	glycosylated hemoglobin (GHB)	856:884	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	98	gly	glycosylated	856:867	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	5	99	theme	model	1726:1730	arg1	group					1732:1736	the model group	1722:1736	the model group (P<0.01)	1722:1745	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	99	theme	model	1726:1730	arg1	P<0.01					1739:1744	P<0.01	1739:1744	P<0.01	1739:1744	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	3	100	dep	linked	1037:1042	arg1	by					1027:1028	by	1027:1028	by	1027:1028	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	101	from	alkaloids	300:308	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	0	102	from	Apoptosis	124:132	arg1	Rabbit					162:167	Diabetic Atherosclerosis Rabbit	137:167	Diabetic Atherosclerosis Rabbit	137:167	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	3	103	theme	immunohistochemical	1160:1178	arg1	method					1180:1185	immunohistochemical method	1160:1185	immunohistochemical method	1160:1185	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	104	theme	CONR	563:566	arg1	group					577:581	CONR low-dose group	563:581	CONR low-dose group (50 mg/kg)	563:592	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	104	theme	CONR	563:566	arg1	mg/kg					587:591	50 mg/kg	584:591	50 mg/kg	584:591	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	9	105	contain	had	2347:2349	arg2	effect					2358:2363	better effect	2351:2363	better effect on serum AGEs	2351:2377	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	105	contain	had	2347:2349	arg1	CONR					2342:2345	Middle-dose CONR	2330:2345	Middle-dose CONR	2330:2345	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	10	106	theme	CONCLUSION	2596:2605	arg1	CONR					2607:2610	CONCLUSION CONR	2596:2610	CONCLUSION CONR	2596:2610	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	1	107	from	Composition	210:220	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	9	108	from	effect	2473:2478	arg1	expression					2487:2496	the expression	2483:2496	the expression of caspase-3, bcl-2 protein, myocardium apoptosis	2483:2546	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	3	109	theme	p-Jun	1192:1196	arg1	kinase					1209:1214	p-Jun N-terminal kinase	1192:1214	p-Jun N-terminal kinase	1192:1214	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	110	theme	DA	370:371	arg1	rabbits					374:380	diabetic atherosclerosis (DA) rabbits	344:380	diabetic atherosclerosis (DA) rabbits	344:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	111	theme	FRA	1814:1816	arg1	levels					1783:1788	the levels	1779:1788	the levels of serum FBG, GHB, GSP, FRA and AR	1779:1823	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	9	112	theme	better	2351:2356	arg1	effect					2358:2363	better effect	2351:2363	better effect on serum AGEs	2351:2377	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	113	theme	low-dose	2571:2578	arg1	P<0.01					2587:2592	P<0.01	2587:2592	P<0.01	2587:2592	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	9	113	theme	low-dose	2571:2578	arg1	group					2580:2584	the CONR low-dose group	2562:2584	the CONR low-dose group (P<0.01)	2562:2593	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	1	114	theme	rabbits	374:380	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	115	theme	GSP	1809:1811	arg1	levels					1783:1788	the levels	1779:1788	the levels of serum FBG, GHB, GSP, FRA and AR	1779:1823	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	9	116	from	effect	2358:2363	arg1	AGEs					2374:2377	serum AGEs	2368:2377	serum AGEs	2368:2377	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	3	117	from	products	988:995	arg1	serum					1007:1011	serum	1007:1011	serum	1007:1011	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	9	118	theme	CONR	2434:2437	arg1	groups					2455:2460	middle-dose CONR and simvastatin groups	2422:2460	middle-dose CONR and simvastatin groups	2422:2460	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	1	119	theme	CONR	527:530	arg1	group					544:548	CONR medium-dose group	527:548	CONR medium-dose group (150 mg/kg)	527:560	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	119	theme	CONR	527:530	arg1	mg/kg					555:559	150 mg/kg	551:559	150 mg/kg	551:559	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	8	120	theme	treatment	2183:2191	arg1	groups					2193:2198	all treatment groups	2179:2198	all treatment groups (P<0.01)	2179:2207	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	8	120	theme	treatment	2183:2191	arg1	P<0.01					2201:2206	P<0.01	2201:2206	P<0.01	2201:2206	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30328567	6	121	theme	GHB	1804:1806	arg1	levels					1783:1788	the levels	1779:1788	the levels of serum FBG, GHB, GSP, FRA and AR	1779:1823	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	7	122	theme	CONR	1945:1948	arg1	group					1959:1963	CONR low-dose group	1945:1963	CONR low-dose group (P<0.01)	1945:1972	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	7	122	theme	CONR	1945:1948	arg1	P<0.01					1966:1971	P<0.01	1966:1971	P<0.01	1966:1971	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	7	123	theme	significant	1922:1932	arg1	decline					1934:1940	a significant decline	1920:1940	a significant decline in CONR low-dose group (P<0.01)	1920:1972	FBG and GHB showed a significant decline in CONR low-dose group (P<0.01).
30328567	1	124	theme	male	397:400	arg1	rabbits					420:426	Sixty male New Zealand white rabbits	391:426	Sixty male New Zealand white rabbits	391:426	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	125	theme	FBG	1799:1801	arg1	levels					1783:1788	the levels	1779:1788	the levels of serum FBG, GHB, GSP, FRA and AR	1779:1823	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	3	126	theme	blood	835:839	arg1	glucose					841:847	fasting blood glucose	827:847	fasting blood glucose (FBG)	827:853	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	126	theme	blood	835:839	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	126	theme	blood	835:839	arg1	FBG					850:852	FBG	850:852	FBG	850:852	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	6	127	theme	CONR	1857:1860	arg1	P<0.01					1892:1897	P<0.01	1892:1897	P<0.01	1892:1897	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	6	127	theme	CONR	1857:1860	arg1	groups					1884:1889	CONR high- and medium-dose groups	1857:1889	CONR high- and medium-dose groups (P<0.01)	1857:1898	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	3	128	theme	glycosylated	856:867	arg1	GHB					881:883	GHB	881:883	GHB	881:883	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	128	theme	glycosylated	856:867	arg1	hemoglobin					869:878	glycosylated hemoglobin	856:878	glycosylated hemoglobin (GHB)	856:884	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	128	theme	glycosylated	856:867	arg1	mediation					803:811	mediation	803:811	mediation for 10 weeks	803:824	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	129	from	saponins	270:277	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	0	130	theme	Notoginseng	42:52	arg1	Composition					0:10	Composition	0:10	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids	0:98	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	1	131	theme	myocardial	320:329	arg1	apoptosis					331:339	myocardial apoptosis	320:339	myocardial apoptosis of diabetic atherosclerosis (DA) rabbits	320:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	132	theme	glycation	974:982	arg1	products					988:995	advanced glycation end products	965:995	advanced glycation end products (AGEs) in serum	965:1011	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	132	theme	glycation	974:982	arg1	AGEs					998:1001	AGEs	998:1001	AGEs	998:1001	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	3	133	theme	bcl-2	1255:1259	arg1	expression					1270:1279	B-cell lymphoma-2 (bcl-2) protein expression	1236:1279	B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue	1236:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	134	theme	CONR	493:496	arg1	group					508:512	CONR high-dose group	493:512	CONR high-dose group (450 mg/kg)	493:524	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	1	134	theme	CONR	493:496	arg1	mg/kg					519:523	450 mg/kg	515:523	450 mg/kg	515:523	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	5	135	theme	control	1496:1502	arg1	group					1504:1508	the control group	1492:1508	the control group	1492:1508	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	0	136	dep	Notoginseng	42:52	arg1	Alkaloids					90:98	Alkaloids	90:98	Alkaloids	90:98	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	0	136	dep	Notoginseng	42:52	arg1	Saponins					60:67	Total Saponins	54:67	Total Saponins	54:67	Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis Rabbit.
30328567	3	137	theme	lymphoma-2	1243:1252	arg1	expression					1270:1279	B-cell lymphoma-2 (bcl-2) protein expression	1236:1279	B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue	1236:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	1	138	theme	atherosclerosis	353:367	arg1	rabbits					374:380	diabetic atherosclerosis (DA) rabbits	344:380	diabetic atherosclerosis (DA) rabbits	344:380	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	6	139	theme	AR	1822:1823	arg1	levels					1783:1788	the levels	1779:1788	the levels of serum FBG, GHB, GSP, FRA and AR	1779:1823	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	1	140	from	10	655:656	arg1	group					666:670	each group	661:670	each group	661:670	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	9	141	theme	middle-dose	2422:2432	arg1	groups					2455:2460	middle-dose CONR and simvastatin groups	2422:2460	middle-dose CONR and simvastatin groups	2422:2460	Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01).
30328567	2	142	theme	high-fat	746:753	arg1	diet					755:758	high-fat diet	746:758	high-fat diet	746:758	DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury.
30328567	5	143	theme	index	1633:1637	arg1	FRA					1532:1534	FRA	1532:1534	FRA	1532:1534	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	expression					1554:1563	the expression	1550:1563	the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI)	1550:1642	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	AR					1537:1538	AR	1537:1538	AR	1537:1538	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	GHB					1522:1524	GHB	1522:1524	GHB	1522:1524	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	GSP					1527:1529	GSP	1527:1529	GSP	1527:1529	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	AGEs					1541:1544	AGEs	1541:1544	AGEs	1541:1544	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	5	143	theme	index	1633:1637	arg1	FBG					1517:1519	serum FBG	1511:1519	serum FBG	1511:1519	RESULTS Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01).
30328567	6	144	theme	model	1766:1770	arg1	group					1772:1776	the model group	1762:1776	the model group	1762:1776	Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01).
30328567	4	145	theme	TdT-mediated	1378:1389	arg1	TUNEL					1414:1418	TUNEL	1414:1418	TUNEL	1414:1418	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	4	145	theme	TdT-mediated	1378:1389	arg1	labeling					1404:1411	TdT-mediated dUTPnick-end labeling	1378:1411	TdT-mediated dUTPnick-end labeling (TUNEL) method	1378:1426	The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated.
30328567	10	146	theme	myocardial	2636:2645	arg1	apoptosis					2647:2655	myocardial apoptosis	2636:2655	myocardial apoptosis on DA rabbits	2636:2669	CONCLUSION CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.
30328567	1	147	theme	total	264:268	arg1	saponins					270:277	Notoginseng total saponins	252:277	Notoginseng total saponins	252:277	OBJECTIVE To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group.
30328567	3	148	theme	protein	1262:1268	arg1	expression					1270:1279	B-cell lymphoma-2 (bcl-2) protein expression	1236:1279	B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue	1236:1300	After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting.
30328567	8	149	theme	abovementioned	2260:2273	arg1	indices					2275:2281	abovementioned indices	2260:2281	abovementioned indices	2260:2281	Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01).
30732755	7	0	theme	loading	992:998	arg1	level					1000:1004	the loading level	988:1004	the loading level of nanoparticles	988:1021	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	3	1	theme	absorption	352:361	arg1	peak					363:366	absorption peak	352:366	absorption peak	352:366	The absorption peak and average size of nanoparticles was approximately 364 nm and 25 nm, respectively.
30732755	4	2	theme	starch	545:550	arg1	matrix					552:557	the starch matrix	541:557	the starch matrix	541:557	It was observed that nanoparticles with less than 3.0 wt% were homogenously dispersed in the starch matrix.
30732755	5	3	theme	43.7	677:680	arg1	%					672:672	%	672:672	%	672:672	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	6	4	from	aggregation	819:829	arg1	p < 0.05					897:904	p < 0.05	897:904	p < 0.05	897:904	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	4	from	aggregation	819:829	arg1	permeability					883:894	increased water vapor permeability	861:894	increased water vapor permeability (p < 0.05)	861:905	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	4	from	aggregation	819:829	arg1	films					851:855	films	851:855	films	851:855	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	5	5	dep	resulted	599:606	arg1	accompanied					683:693	accompanied	683:693	accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05)	683:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	5	6	from	4.11	737:740	arg1	increase					703:710	an increase	700:710	an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05)	700:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	5	6	from	4.11	737:740	arg1	strength					723:730	tensile strength	715:730	tensile strength from 4.11 to 12.79 MPa (p < 0.05)	715:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	3	7	theme	nanoparticles	388:400	arg1	peak					363:366	absorption peak	352:366	absorption peak	352:366	The absorption peak and average size of nanoparticles was approximately 364 nm and 25 nm, respectively.
30732755	3	7	theme	nanoparticles	388:400	arg1	size					380:383	average size	372:383	average size	372:383	The absorption peak and average size of nanoparticles was approximately 364 nm and 25 nm, respectively.
30732755	5	8	theme	water	638:642	arg1	permeability					650:661	water vapor permeability	638:661	water vapor permeability	638:661	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	7	9	contain	had	1040:1042	arg1	films					1034:1038	these films	1028:1038	these films	1028:1038	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	7	9	contain	had	1040:1042	arg2	suppression					1053:1063	stronger suppression	1044:1063	stronger suppression	1044:1063	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	3	10	theme	average	372:378	arg1	size					380:383	average size	372:383	average size	372:383	The absorption peak and average size of nanoparticles was approximately 364 nm and 25 nm, respectively.
30732755	5	11	theme	permeability	650:661	arg1	reduction					625:633	a substantial reduction	611:633	a substantial reduction of water vapor permeability from 51.0% to 43.7%	611:681	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	5	12	theme	vapor	644:648	arg1	permeability					650:661	water vapor permeability	638:661	water vapor permeability	638:661	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	1	13	theme	modified	189:196	arg1	matrix					205:210	the modified starch matrix	185:210	the modified starch matrix	185:210	This work dealt with the antimicrobial nanoparticles and incorporated them into the modified starch matrix to prepare composite films.
30732755	7	14	theme	starch-based	938:949	arg1	films					951:955	starch-based films	938:955	starch-based films	938:955	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	1	15	theme	starch	198:203	arg1	matrix					205:210	the modified starch matrix	185:210	the modified starch matrix	185:210	This work dealt with the antimicrobial nanoparticles and incorporated them into the modified starch matrix to prepare composite films.
30732755	6	16	theme	vapor	877:881	arg1	p < 0.05					897:904	p < 0.05	897:904	p < 0.05	897:904	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	16	theme	vapor	877:881	arg1	permeability					883:894	increased water vapor permeability	861:894	increased water vapor permeability (p < 0.05)	861:905	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	5	17	theme	51.0	668:671	arg1	%					672:672	%	672:672	%	672:672	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	7	18	theme	films	951:955	arg1	activity					926:933	The antimicrobial activity	908:933	The antimicrobial activity of starch-based films	908:955	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	2	19	theme	phase	341:345	arg1	formation					318:326	formation	318:326	formation of hexagonal phase	318:345	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30732755	4	20	with	nanoparticles	473:485	arg1	%					508:508	less than 3.0 wt%	492:508	less than 3.0 wt%	492:508	It was observed that nanoparticles with less than 3.0 wt% were homogenously dispersed in the starch matrix.
30732755	5	21	theme	tensile	715:721	arg1	strength					723:730	tensile strength	715:730	tensile strength from 4.11 to 12.79 MPa (p < 0.05)	715:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	5	22	theme	substantial	613:623	arg1	reduction					625:633	a substantial reduction	611:633	a substantial reduction of water vapor permeability from 51.0% to 43.7%	611:681	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	2	23	theme	hexagonal	331:339	arg1	phase					341:345	hexagonal phase	331:345	hexagonal phase	331:345	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30732755	5	24	theme	strength	723:730	arg1	increase					703:710	an increase	700:710	an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05)	700:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	6	25	theme	water	871:875	arg1	p < 0.05					897:904	p < 0.05	897:904	p < 0.05	897:904	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	25	theme	water	871:875	arg1	permeability					883:894	increased water vapor permeability	861:894	increased water vapor permeability (p < 0.05)	861:905	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	1	26	theme	composite	223:231	arg1	films					233:237	composite films	223:237	composite films	223:237	This work dealt with the antimicrobial nanoparticles and incorporated them into the modified starch matrix to prepare composite films.
30732755	0	27	theme	antimicrobial	41:53	arg1	films					65:69	starch-based antimicrobial composite films	28:69	starch-based antimicrobial composite films using ZnO-chitosan nanoparticles	28:102	Improving the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles.
30732755	2	28	with	nanoparticles	257:269	arg1	ZnO					285:287	57.3 wt% ZnO	276:287	57.3 wt% ZnO	276:287	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30732755	3	29	dep	peak	363:366	arg1	The					348:350	The	348:350	The	348:350	The absorption peak and average size of nanoparticles was approximately 364 nm and 25 nm, respectively.
30732755	6	30	theme	increased	861:869	arg1	p < 0.05					897:904	p < 0.05	897:904	p < 0.05	897:904	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	30	theme	increased	861:869	arg1	permeability					883:894	increased water vapor permeability	861:894	increased water vapor permeability (p < 0.05)	861:905	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	0	31	theme	starch-based	28:39	arg1	films					65:69	starch-based antimicrobial composite films	28:69	starch-based antimicrobial composite films using ZnO-chitosan nanoparticles	28:102	Improving the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles.
30732755	5	32	from	%	681:681	arg1	reduction					625:633	a substantial reduction	611:633	a substantial reduction of water vapor permeability from 51.0% to 43.7%	611:681	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	5	33	dep	%	672:672	arg1	to					674:675	to	674:675	to	674:675	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	0	34	theme	films	65:69	arg1	properties					14:23	the properties	10:23	the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles	10:102	Improving the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles.
30732755	7	35	theme	stronger	1044:1051	arg1	suppression					1053:1063	stronger suppression	1044:1063	stronger suppression	1044:1063	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	2	36	theme	ZnO-chitosan	244:255	arg1	nanoparticles					257:269	The ZnO-chitosan nanoparticles	240:269	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO	240:287	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30732755	5	37	theme	nanoparticles	576:588	arg1	increase					564:571	The increase	560:571	The increase of nanoparticles in films	560:597	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	0	38	theme	composite	55:63	arg1	films					65:69	starch-based antimicrobial composite films	28:69	starch-based antimicrobial composite films using ZnO-chitosan nanoparticles	28:102	Improving the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles.
30732755	7	39	theme	nanoparticles	1009:1021	arg1	level					1000:1004	the loading level	988:1004	the loading level of nanoparticles	988:1021	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	1	40	theme	antimicrobial	130:142	arg1	nanoparticles					144:156	the antimicrobial nanoparticles	126:156	the antimicrobial nanoparticles	126:156	This work dealt with the antimicrobial nanoparticles and incorporated them into the modified starch matrix to prepare composite films.
30732755	6	41	theme	higher	777:782	arg1	addition					784:791	higher addition	777:791	higher addition (4.0-5.0 wt%)	777:805	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	41	theme	higher	777:782	arg1	%					804:804	4.0-5.0 wt%	794:804	4.0-5.0 wt%	794:804	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	6	42	theme	nanoparticles	834:846	arg1	aggregation					819:829	aggregation	819:829	aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05)	819:905	Moreover, higher addition (4.0-5.0 wt%) resulted in aggregation of nanoparticles in films and increased water vapor permeability (p < 0.05).
30732755	7	43	theme	antimicrobial	912:924	arg1	activity					926:933	The antimicrobial activity	908:933	The antimicrobial activity of starch-based films	908:955	The antimicrobial activity of starch-based films was positively correlated with the loading level of nanoparticles, and these films had stronger suppression in Gram-positive S. aureus than Gram-negative E. coli.
30732755	5	44	from	increase	703:710	arg1	4.11					737:740	4.11	737:740	4.11 to 12.79 MPa (p < 0.05)	737:764	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	0	45	theme	ZnO-chitosan	77:88	arg1	nanoparticles					90:102	ZnO-chitosan nanoparticles	77:102	ZnO-chitosan nanoparticles	77:102	Improving the properties of starch-based antimicrobial composite films using ZnO-chitosan nanoparticles.
30732755	2	46	theme	%	283:283	arg1	ZnO					285:287	57.3 wt% ZnO	276:287	57.3 wt% ZnO	276:287	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30732755	5	47	from	increase	564:571	arg1	films					593:597	films	593:597	films	593:597	The increase of nanoparticles in films resulted in a substantial reduction of water vapor permeability from 51.0% to 43.7% accompanied with an increase of tensile strength from 4.11 to 12.79 MPa (p < 0.05).
30732755	2	48	theme	57.3 wt	276:282	arg1	ZnO					285:287	57.3 wt% ZnO	276:287	57.3 wt% ZnO	276:287	The ZnO-chitosan nanoparticles with 57.3 wt% ZnO were sol-gel synthesized for formation of hexagonal phase.
30695724	3	0	theme	ratio	738:742	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	0	theme	ratio	738:742	arg1	30 mL/g					744:750	solvent-to-material ratio 30 mL/g	718:750	solvent-to-material ratio 30 mL/g	718:750	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	1	1	theme	assisted	171:178	arg1	UMAA					188:191	UMAA	188:191	UMAA	188:191	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	1	1	theme	assisted	171:178	arg1	alkali					180:185	Ultrasonic-Microwave assisted alkali	150:185	Ultrasonic-Microwave assisted alkali (UMAA)	150:192	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	0	2	theme	corn	89:92	arg1	bran					94:97	the corn bran	85:97	the corn bran using response surface methodology	85:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	3	3	theme	0.30 mol/L	706:715	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	3	theme	0.30 mol/L	706:715	arg1	concentration					672:684	concentration	672:684	concentration of sodium hydroxide 0.30 mol/L	672:715	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	0	4	theme	response	105:112	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	0	5	from	Optimization	0:11	arg1	bran					94:97	the corn bran	85:97	the corn bran using response surface methodology	85:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	5	6	theme	galacturonic	1012:1023	arg1	acid					1025:1028	galacturonic acid	1012:1028	galacturonic acid	1012:1028	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	6	theme	galacturonic	1012:1023	arg1	xylose					934:939	xylose	934:939	xylose	934:939	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	1	7	theme	corn	266:269	arg1	bran					271:274	the corn bran	262:274	the corn bran	262:274	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	2	8	theme	single	539:544	arg1	experiments					553:563	single factor experiments	539:563	single factor experiments	539:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	3	9	theme	ultrasonic-microwave	753:772	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	9	theme	ultrasonic-microwave	753:772	arg1	25 min					790:795	ultrasonic-microwave synergetic time 25 min	753:795	ultrasonic-microwave synergetic time 25 min	753:795	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	2	10	theme	various	281:287	arg1	time					422:425	ultrasonic-microwave synergetic time	390:425	ultrasonic-microwave synergetic time	390:425	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	10	theme	various	281:287	arg1	ratio					380:384	solvent-to-material ratio	360:384	solvent-to-material ratio	360:384	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	10	theme	various	281:287	arg1	concentration					325:337	concentration	325:337	concentration of sodium hydroxide	325:357	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	10	theme	various	281:287	arg1	factors					289:295	The various factors	277:295	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time	277:425	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	10	theme	various	281:287	arg1	power					318:322	ultrasonic power	307:322	ultrasonic power	307:322	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	7	11	theme	antioxidant	1332:1342	arg1	WUAX					1344:1347	antioxidant WUAX	1332:1347	antioxidant WUAX from corn bran	1332:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	3	12	theme	synergetic	774:783	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	12	theme	synergetic	774:783	arg1	25 min					790:795	ultrasonic-microwave synergetic time 25 min	753:795	ultrasonic-microwave synergetic time 25 min	753:795	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	5	13	theme	glucuronic	992:1001	arg1	acid					1003:1006	glucuronic acid	992:1006	glucuronic acid	992:1006	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	13	theme	glucuronic	992:1001	arg1	xylose					934:939	xylose	934:939	xylose	934:939	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	14	theme	Chemical	867:874	arg1	analysis					888:895	Chemical composition analysis	867:895	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	4	15	theme	characteristic	827:840	arg1	peaks					842:846	the characteristic peaks	823:846	the characteristic peaks of polysaccharide	823:864	FT-IR analysis processed the characteristic peaks of polysaccharide.
30695724	0	16	theme	surface	114:120	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	2	17	theme	Box-Behnken	487:497	arg1	BBD					507:509	BBD	507:509	BBD	507:509	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	17	theme	Box-Behnken	487:497	arg1	design					499:504	Box-Behnken design	487:504	Box-Behnken design (BBD)	487:510	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	5	18	from	ratio	1036:1040	arg1	rhamnose					973:980	rhamnose	973:980	rhamnose	973:980	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	mannose					983:989	mannose	983:989	mannose	983:989	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	acid					1025:1028	galacturonic acid	1012:1028	galacturonic acid	1012:1028	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	glucose					953:959	glucose	953:959	glucose	953:959	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	galactose					962:970	galactose	962:970	galactose	962:970	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	acid					1003:1006	glucuronic acid	992:1006	glucuronic acid	992:1006	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	5	18	from	ratio	1036:1040	arg1	xylose					934:939	xylose	934:939	xylose	934:939	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	2	19	theme	synergetic	411:420	arg1	time					422:425	ultrasonic-microwave synergetic time	390:425	ultrasonic-microwave synergetic time	390:425	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	5	20	theme	composition	876:886	arg1	analysis					888:895	Chemical composition analysis	867:895	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	3	21	theme	maximum	594:600	arg1	yield					607:611	the maximum WUAX yield	590:611	the maximum WUAX yield of 27.78 ± 0.17%	590:628	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	2	22	theme	ultrasonic-microwave	390:409	arg1	time					422:425	ultrasonic-microwave synergetic time	390:425	ultrasonic-microwave synergetic time	390:425	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	0	23	theme	assisted	37:44	arg1	extraction					53:62	ultrasonic-microwave assisted alkali extraction	16:62	ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology	16:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	7	24	theme	broad	1407:1411	arg1	development					1413:1423	broad development	1407:1423	broad development	1407:1423	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	7	25	from	extraction	1318:1327	arg1	bran					1359:1362	corn bran	1354:1362	corn bran	1354:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	2	26	theme	ultrasonic	307:316	arg1	power					318:322	ultrasonic power	307:322	ultrasonic power	307:322	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	27	theme	surface	454:460	arg1	methodology					462:472	response surface methodology	445:472	response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments	445:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	0	28	theme	ultrasonic-microwave	16:35	arg1	extraction					53:62	ultrasonic-microwave assisted alkali extraction	16:62	ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology	16:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	3	29	theme	time	785:788	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	29	theme	time	785:788	arg1	25 min					790:795	ultrasonic-microwave synergetic time 25 min	753:795	ultrasonic-microwave synergetic time 25 min	753:795	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	1	30	from	bran	271:274	arg1	extract					209:215	extract	209:215	extract	209:215	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	0	31	from	bran	94:97	arg1	extraction					53:62	ultrasonic-microwave assisted alkali extraction	16:62	ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology	16:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	0	31	from	bran	94:97	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.	0:133	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	6	32	from	effect	1226:1231	arg1	radical					1241:1247	DPPH radical	1236:1247	DPPH radical	1236:1247	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	2	33	theme	response	445:452	arg1	methodology					462:472	response surface methodology	445:472	response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments	445:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	0	34	theme	extraction	53:62	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.	0:133	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	6	35	theme	chelating	1187:1195	arg1	activity					1197:1204	strong Fe2+ chelating activity	1175:1204	strong Fe2+ chelating activity	1175:1204	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	6	36	theme	activity	1124:1131	arg1	assay					1133:1137	the antioxidant activity assay	1108:1137	the antioxidant activity assay in vitro	1108:1146	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	7	37	theme	corn	1354:1357	arg1	bran					1359:1362	corn bran	1354:1362	corn bran	1354:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	0	38	theme	alkali	46:51	arg1	extraction					53:62	ultrasonic-microwave assisted alkali extraction	16:62	ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology	16:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	2	39	theme	experiments	553:563	arg1	results					528:534	the results	524:534	the results of single factor experiments	524:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	3	40	theme	ultrasonic	648:657	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	40	theme	ultrasonic	648:657	arg1	30 mL/g					744:750	solvent-to-material ratio 30 mL/g	718:750	solvent-to-material ratio 30 mL/g	718:750	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	40	theme	ultrasonic	648:657	arg1	25 min					790:795	ultrasonic-microwave synergetic time 25 min	753:795	ultrasonic-microwave synergetic time 25 min	753:795	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	40	theme	ultrasonic	648:657	arg1	concentration					672:684	concentration	672:684	concentration of sodium hydroxide 0.30 mol/L	672:715	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	5	41	theme	analysis	888:895	arg1	WUAX					906:909	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	2	42	theme	results	528:534	arg1	basis					515:519	basis	515:519	basis of the results of single factor experiments	515:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	0	43	theme	arabinoxylan	67:78	arg1	extraction					53:62	ultrasonic-microwave assisted alkali extraction	16:62	ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology	16:132	Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology.
30695724	2	44	theme	factor	546:551	arg1	experiments					553:563	single factor experiments	539:563	single factor experiments	539:563	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	3	45	theme	power	659:663	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	45	theme	power	659:663	arg1	30 mL/g					744:750	solvent-to-material ratio 30 mL/g	718:750	solvent-to-material ratio 30 mL/g	718:750	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	45	theme	power	659:663	arg1	25 min					790:795	ultrasonic-microwave synergetic time 25 min	753:795	ultrasonic-microwave synergetic time 25 min	753:795	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	45	theme	power	659:663	arg1	concentration					672:684	concentration	672:684	concentration of sodium hydroxide 0.30 mol/L	672:715	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	46	theme	WUAX	602:605	arg1	yield					607:611	the maximum WUAX yield	590:611	the maximum WUAX yield of 27.78 ± 0.17%	590:628	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	7	47	from	bran	1359:1362	arg1	extraction					1318:1327	extraction	1318:1327	extraction of antioxidant WUAX from corn bran	1318:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	7	47	from	bran	1359:1362	arg1	WUAX					1344:1347	antioxidant WUAX	1332:1347	antioxidant WUAX from corn bran	1332:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	2	48	theme	hydroxide	349:357	arg1	time					422:425	ultrasonic-microwave synergetic time	390:425	ultrasonic-microwave synergetic time	390:425	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	48	theme	hydroxide	349:357	arg1	ratio					380:384	solvent-to-material ratio	360:384	solvent-to-material ratio	360:384	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	48	theme	hydroxide	349:357	arg1	concentration					325:337	concentration	325:337	concentration of sodium hydroxide	325:357	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	2	48	theme	hydroxide	349:357	arg1	power					318:322	ultrasonic power	307:322	ultrasonic power	307:322	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	3	49	theme	sodium	689:694	arg1	0.30 mol/L					706:715	sodium hydroxide 0.30 mol/L	689:715	sodium hydroxide 0.30 mol/L	689:715	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	6	50	theme	good	1210:1213	arg1	effect					1226:1231	good scavenging effect	1210:1231	good scavenging effect on DPPH radical	1210:1247	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	6	51	from	activity	1197:1204	arg1	radical					1241:1247	DPPH radical	1236:1247	DPPH radical	1236:1247	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	2	52	theme	sodium	342:347	arg1	hydroxide					349:357	sodium hydroxide	342:357	sodium hydroxide	342:357	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	5	53	theme	38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4	1045:1078	arg1	ratio					1036:1040	a ratio	1034:1040	a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively	1034:1092	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	3	54	theme	hydroxide	696:704	arg1	0.30 mol/L					706:715	sodium hydroxide 0.30 mol/L	689:715	sodium hydroxide 0.30 mol/L	689:715	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	6	55	theme	Fe2+	1182:1185	arg1	activity					1197:1204	strong Fe2+ chelating activity	1175:1204	strong Fe2+ chelating activity	1175:1204	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	4	56	theme	FT-IR	798:802	arg1	analysis					804:811	FT-IR analysis	798:811	FT-IR analysis	798:811	FT-IR analysis processed the characteristic peaks of polysaccharide.
30695724	7	57	theme	WUAX	1344:1347	arg1	extraction					1318:1327	extraction	1318:1327	extraction of antioxidant WUAX from corn bran	1318:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	5	58	theme	indicted	897:904	arg1	WUAX					906:909	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX	867:909	Chemical composition analysis indicted WUAX was mainly composed of xylose, arabinose, glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, at a ratio of 38.3:28.2:22.3:5.1:2.5:0.7:1.5:1.4, respectively.
30695724	7	59	dep	development	1413:1423	arg1	prospects					1441:1449	prospects	1441:1449	prospects	1441:1449	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	6	60	theme	strong	1175:1180	arg1	activity					1197:1204	strong Fe2+ chelating activity	1175:1204	strong Fe2+ chelating activity	1175:1204	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	1	61	theme	water-unextractable	217:235	arg1	WUAX					251:254	WUAX	251:254	WUAX	251:254	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	1	61	theme	water-unextractable	217:235	arg1	arabinoxylan					237:248	water-unextractable arabinoxylan	217:248	water-unextractable arabinoxylan (WUAX)	217:255	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	3	62	theme	%	628:628	arg1	yield					607:611	the maximum WUAX yield	590:611	the maximum WUAX yield of 27.78 ± 0.17%	590:628	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	6	63	theme	antioxidant	1112:1122	arg1	assay					1133:1137	the antioxidant activity assay	1108:1137	the antioxidant activity assay in vitro	1108:1146	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	2	64	theme	solvent-to-material	360:378	arg1	ratio					380:384	solvent-to-material ratio	360:384	solvent-to-material ratio	360:384	The various factors including ultrasonic power, concentration of sodium hydroxide, solvent-to-material ratio and ultrasonic-microwave synergetic time were optimized by response surface methodology coupled with Box-Behnken design (BBD) on basis of the results of single factor experiments.
30695724	6	65	theme	DPPH	1236:1239	arg1	radical					1241:1247	DPPH radical	1236:1247	DPPH radical	1236:1247	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	6	66	theme	scavenging	1215:1224	arg1	effect					1226:1231	good scavenging effect	1210:1231	good scavenging effect on DPPH radical	1210:1247	In addition, the antioxidant activity assay in vitro showed that WUAX processed strong Fe2+ chelating activity and good scavenging effect on DPPH radical.
30695724	3	67	theme	solvent-to-material	718:736	arg1	500 W					665:669	ultrasonic power 500 W	648:669	ultrasonic power 500 W	648:669	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	3	67	theme	solvent-to-material	718:736	arg1	30 mL/g					744:750	solvent-to-material ratio 30 mL/g	718:750	solvent-to-material ratio 30 mL/g	718:750	The results showed that the maximum WUAX yield of 27.78 ± 0.17% was obtained with ultrasonic power 500 W, concentration of sodium hydroxide 0.30 mol/L, solvent-to-material ratio 30 mL/g, ultrasonic-microwave synergetic time 25 min.
30695724	4	68	theme	polysaccharide	851:864	arg1	peaks					842:846	the characteristic peaks	823:846	the characteristic peaks of polysaccharide	823:864	FT-IR analysis processed the characteristic peaks of polysaccharide.
30695724	1	69	theme	Ultrasonic-Microwave	150:169	arg1	UMAA					188:191	UMAA	188:191	UMAA	188:191	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	1	69	theme	Ultrasonic-Microwave	150:169	arg1	alkali					180:185	Ultrasonic-Microwave assisted alkali	150:185	Ultrasonic-Microwave assisted alkali (UMAA)	150:192	In this study, Ultrasonic-Microwave assisted alkali (UMAA) was applied to extract water-unextractable arabinoxylan (WUAX) from the corn bran.
30695724	7	70	theme	effective	1297:1305	arg1	UMAA					1286:1289	the UMAA	1282:1289	the UMAA	1282:1289	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
30695724	7	70	theme	effective	1297:1305	arg1	method					1307:1312	an effective method	1294:1312	an effective method for extraction of antioxidant WUAX from corn bran	1294:1362	These results clearly indicated the UMAA is an effective method for extraction of antioxidant WUAX from corn bran and the polysaccharides could be exhibited broad development and application prospects.
29421049	4	0	theme	chromatography	831:844	arg1	determination					852:864	Size exclusion chromatography (SEC) determination	816:864	Size exclusion chromatography (SEC) determination	816:864	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	1	theme	preliminary	609:619	arg1	study					631:635	preliminary structure study	609:635	preliminary structure study	609:635	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	7	2	theme	optimum	1370:1376	arg1	polysaccharide					1378:1391	the optimum polysaccharide	1366:1391	the optimum polysaccharide	1366:1391	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	5	3	from	galactose	1160:1168	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	3	4	theme	acid	554:557	arg1	material					572:579	31.8 mg ascorbic acid equivalent/g material	537:579	31.8 mg ascorbic acid equivalent/g material	537:579	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	4	5	theme	Size	816:819	arg1	chromatography					831:844	Size exclusion chromatography	816:844	Size exclusion chromatography (SEC) determination	816:864	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	5	theme	Size	816:819	arg1	SEC					847:849	SEC	847:849	SEC	847:849	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	5	6	from	mannose	1151:1157	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	1	7	theme	ultrasound-assisted	199:217	arg1	system					219:224	ultrasound-assisted system	199:224	ultrasound-assisted system	199:224	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	5	8	from	glucose	1181:1187	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	2	9	theme	independent	333:343	arg1	variables					345:353	Three independent variables	327:353	Three independent variables	327:353	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	5	10	from	rhamnose	1171:1178	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	6	11	theme	that	1326:1329	arg1	typical					1315:1321	typical	1315:1321	typical	1315:1321	This sugar composition was typical of that of a galactoarabinan.
29421049	1	12	theme	extraction	238:247	arg1	yield					249:253	extraction yield	238:253	extraction yield	238:253	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	2	13	dep	studied	360:366	arg1	pH					391:392	pH	391:392	pH	391:392	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	13	dep	studied	360:366	arg1	time					382:385	time	382:385	time	382:385	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	13	dep	studied	360:366	arg1	temperature					369:379	temperature	369:379	temperature	369:379	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	5	14	from	xylose	1193:1198	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	15	theme	uronic	1054:1059	arg1	content					1066:1072	uronic acid content	1054:1072	uronic acid content (47.5%)	1054:1080	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	15	theme	uronic	1054:1059	arg1	%					1079:1079	47.5%	1075:1079	47.5%	1075:1079	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	1	16	theme	Box-Behnken	156:166	arg1	BBD					176:178	BBD	176:178	BBD	176:178	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	1	16	theme	Box-Behnken	156:166	arg1	design					168:173	Box-Behnken design	156:173	Box-Behnken design (BBD)	156:179	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	5	17	theme	%	1239:1239	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	4	18	theme	Nuclear	728:734	arg1	resonance					745:753	Nuclear magnetic resonance	728:753	Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC))	728:806	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	19	theme	molecular	920:928	arg1	weight					930:935	an average molecular weight	909:935	an average molecular weight of 240 kDa	909:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	0	20	from	Suaeda	56:61	arg1	extraction					10:19	Optimized extraction	0:19	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa	0:71	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	5	21	theme	%	1254:1254	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	22	theme	neutral	1086:1092	arg1	monosaccharides					1094:1108	neutral monosaccharides	1086:1108	neutral monosaccharides (52.5%)	1086:1116	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	22	theme	neutral	1086:1092	arg1	%					1115:1115	52.5%	1111:1115	52.5%	1111:1115	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	23	theme	composition	958:968	arg1	analyses					970:977	Chemical composition analyses	949:977	Chemical composition analyses	949:977	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	0	24	theme	Optimized	0:8	arg1	extraction					10:19	Optimized extraction	0:19	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa	0:71	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	1	25	theme	Suaeda	309:314	arg1	fruticosa					316:324	Suaeda fruticosa	309:324	Suaeda fruticosa	309:324	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	4	26	contain	had	905:907	arg1	polysaccharide					880:893	polysaccharide	880:893	polysaccharide extracted	880:903	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	26	contain	had	905:907	arg2	weight					930:935	an average molecular weight	909:935	an average molecular weight of 240 kDa	909:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	27	theme	13C	764:766	arg1	1H					760:761	1H	760:761	1H	760:761	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	27	theme	13C	764:766	arg1	NMR					768:770	13C NMR	764:770	13C NMR	764:770	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	2	28	with	temperature	369:379	arg1	settings					407:414	optimal settings	399:414	optimal settings 90 °C, 37 min and 2.9, respectively	399:450	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	28	with	temperature	369:379	arg1	37 min					423:428	37 min	423:428	37 min	423:428	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	28	with	temperature	369:379	arg1	90 °C					416:420	90 °C	416:420	90 °C	416:420	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	0	29	dep	leaves	73:78	arg1	Characterization					81:96	Characterization	81:96	Characterization	81:96	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	0	29	dep	leaves	73:78	arg1	anti-inflammatory					112:128	anti-inflammatory	112:128	anti-inflammatory	112:128	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	0	29	dep	leaves	73:78	arg1	activities					144:153	analgesic activities	134:153	analgesic activities	134:153	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	0	29	dep	leaves	73:78	arg1	antioxidant					99:109	antioxidant	99:109	antioxidant	99:109	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	0	30	theme	polysaccharide	36:49	arg1	extraction					10:19	Optimized extraction	0:19	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa	0:71	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	2	31	with	time	382:385	arg1	settings					407:414	optimal settings	399:414	optimal settings 90 °C, 37 min and 2.9, respectively	399:450	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	31	with	time	382:385	arg1	37 min					423:428	37 min	423:428	37 min	423:428	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	31	with	time	382:385	arg1	90 °C					416:420	90 °C	416:420	90 °C	416:420	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	0	32	dep	Suaeda	56:61	arg1	fruticosa					63:71	Suaeda fruticosa	56:71	Suaeda fruticosa	56:71	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	8	33	theme	extracted	1501:1509	arg1	polysaccharide					1511:1524	extracted polysaccharide	1501:1524	extracted polysaccharide	1501:1524	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	6	34	theme	sugar	1293:1297	arg1	composition					1299:1309	This sugar composition	1288:1309	This sugar composition	1288:1309	This sugar composition was typical of that of a galactoarabinan.
29421049	2	35	with	pH	391:392	arg1	settings					407:414	optimal settings	399:414	optimal settings 90 °C, 37 min and 2.9, respectively	399:450	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	35	with	pH	391:392	arg1	37 min					423:428	37 min	423:428	37 min	423:428	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	35	with	pH	391:392	arg1	90 °C					416:420	90 °C	416:420	90 °C	416:420	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	5	36	theme	pectin-like	1022:1032	arg1	polysaccharide					1034:1047	a pectin-like polysaccharide	1020:1047	a pectin-like polysaccharide with uronic acid content (47.5%)	1020:1080	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	36	theme	pectin-like	1022:1032	arg1	polysaccharide					1001:1014	extracted polysaccharide	991:1014	extracted polysaccharide	991:1014	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	7	37	theme	activity	1441:1448	arg1	effect					1450:1455	antioxidant activity effect	1429:1455	antioxidant activity effect	1429:1455	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	3	38	theme	total	495:499	arg1	activity					513:520	total antioxidant activity	495:520	total antioxidant activity	495:520	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	7	39	theme	significant	1403:1413	arg1	potentials					1415:1424	significant potentials	1403:1424	significant potentials in antioxidant activity effect	1403:1455	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	4	40	dep	spectroscopy	703:714	arg1	methods					808:814	methods	808:814	methods	808:814	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	8	41	theme	good	1537:1540	arg1	effect					1560:1565	a good anti-inflammatory effect	1535:1565	a good anti-inflammatory effect	1535:1565	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	4	42	theme	1H	785:786	arg1	1H					781:782	1H	781:782	1H	781:782	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	42	theme	1H	785:786	arg1	COSY					788:791	1H COSY	785:791	1H COSY	785:791	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	2	43	theme	optimal	399:405	arg1	settings					407:414	optimal settings	399:414	optimal settings 90 °C, 37 min and 2.9, respectively	399:450	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	43	theme	optimal	399:405	arg1	37 min					423:428	37 min	423:428	37 min	423:428	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	43	theme	optimal	399:405	arg1	90 °C					416:420	90 °C	416:420	90 °C	416:420	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	8	44	theme	antinociceptive	1613:1627	arg1	activities					1629:1638	central antinociceptive activities	1605:1638	a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities	1535:1638	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	1	45	theme	total	259:263	arg1	ability					277:283	total antioxidant ability	259:283	total antioxidant ability	259:283	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	7	46	from	potentials	1415:1424	arg1	effect					1450:1455	antioxidant activity effect	1429:1455	antioxidant activity effect	1429:1455	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	5	47	theme	extracted	991:999	arg1	polysaccharide					1034:1047	a pectin-like polysaccharide	1020:1047	a pectin-like polysaccharide with uronic acid content (47.5%)	1020:1080	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	47	theme	extracted	991:999	arg1	polysaccharide					1001:1014	extracted polysaccharide	991:1014	extracted polysaccharide	991:1014	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	48	theme	%	1271:1271	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	4	49	theme	exclusion	821:829	arg1	chromatography					831:844	Size exclusion chromatography	816:844	Size exclusion chromatography (SEC) determination	816:864	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	49	theme	exclusion	821:829	arg1	SEC					847:849	SEC	847:849	SEC	847:849	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	3	50	theme	equivalent/g	559:570	arg1	material					572:579	31.8 mg ascorbic acid equivalent/g material	537:579	31.8 mg ascorbic acid equivalent/g material	537:579	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	1	51	theme	polysaccharides	288:302	arg1	yield					249:253	extraction yield	238:253	extraction yield	238:253	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	1	51	theme	polysaccharides	288:302	arg1	ability					277:283	total antioxidant ability	259:283	total antioxidant ability	259:283	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	4	52	theme	structure	621:629	arg1	study					631:635	preliminary structure study	609:635	preliminary structure study	609:635	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	3	53	theme	ascorbic	545:552	arg1	material					572:579	31.8 mg ascorbic acid equivalent/g material	537:579	31.8 mg ascorbic acid equivalent/g material	537:579	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	1	54	from	fruticosa	316:324	arg1	yield					249:253	extraction yield	238:253	extraction yield	238:253	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	1	54	from	fruticosa	316:324	arg1	polysaccharides					288:302	polysaccharides	288:302	polysaccharides from Suaeda fruticosa	288:324	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	1	54	from	fruticosa	316:324	arg1	ability					277:283	total antioxidant ability	259:283	total antioxidant ability	259:283	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	4	55	theme	UV-vis	659:664	arg1	Fourier					676:682	Fourier	676:682	Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa	676:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	55	theme	UV-vis	659:664	arg1	analysis					666:673	UV-vis analysis	659:673	UV-vis analysis	659:673	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	1	56	from	ability	277:283	arg1	fruticosa					316:324	Suaeda fruticosa	309:324	Suaeda fruticosa	309:324	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	4	57	theme	magnetic	736:743	arg1	resonance					745:753	Nuclear magnetic resonance	728:753	Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC))	728:806	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	5	58	theme	%	1232:1232	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	1	59	from	yield	249:253	arg1	fruticosa					316:324	Suaeda fruticosa	309:324	Suaeda fruticosa	309:324	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
29421049	5	60	theme	acid	1061:1064	arg1	content					1066:1072	uronic acid content	1054:1072	uronic acid content (47.5%)	1054:1080	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	60	theme	acid	1061:1064	arg1	%					1079:1079	47.5%	1075:1079	47.5%	1075:1079	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	4	61	dep	2D	777:778	arg1	1H					781:782	1H	781:782	1H	781:782	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	61	dep	2D	777:778	arg1	COSY					788:791	1H COSY	785:791	1H COSY	785:791	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	61	dep	2D	777:778	arg1	HSQC					801:804	1H/13C HSQC	794:804	1H/13C HSQC	794:804	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	62	theme	240 kDa	940:946	arg1	weight					930:935	an average molecular weight	909:935	an average molecular weight of 240 kDa	909:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	5	63	theme	molar	1207:1211	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	7	64	contain	possesses	1393:1401	arg1	polysaccharide					1378:1391	the optimum polysaccharide	1366:1391	the optimum polysaccharide	1366:1391	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	7	64	contain	possesses	1393:1401	arg2	potentials					1415:1424	significant potentials	1403:1424	significant potentials in antioxidant activity effect	1403:1455	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	0	65	theme	analgesic	134:142	arg1	Characterization					81:96	Characterization	81:96	Characterization	81:96	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	0	65	theme	analgesic	134:142	arg1	activities					144:153	analgesic activities	134:153	analgesic activities	134:153	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	4	66	theme	average	912:918	arg1	weight					930:935	an average molecular weight	909:935	an average molecular weight of 240 kDa	909:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	5	67	theme	Chemical	949:956	arg1	analyses					970:977	Chemical composition analyses	949:977	Chemical composition analyses	949:977	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	68	with	polysaccharide	1034:1047	arg1	content					1066:1072	uronic acid content	1054:1072	uronic acid content (47.5%)	1054:1080	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	5	68	with	polysaccharide	1034:1047	arg1	%					1079:1079	47.5%	1075:1079	47.5%	1075:1079	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	4	69	dep	1D	756:757	arg1	1H					760:761	1H	760:761	1H	760:761	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	69	dep	1D	756:757	arg1	NMR					768:770	13C NMR	764:770	13C NMR	764:770	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	8	70	theme	pharmacological	1468:1482	arg1	investigation					1484:1496	pharmacological investigation	1468:1496	pharmacological investigation of extracted polysaccharide	1468:1524	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	0	71	theme	pectin-like	24:34	arg1	polysaccharide					36:49	pectin-like polysaccharide	24:49	pectin-like polysaccharide	24:49	Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities.
29421049	4	72	dep	resonance	745:753	arg1	1D					756:757	1D	756:757	1D (1H, 13C NMR)	756:771	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	72	dep	resonance	745:753	arg1	2D					777:778	2D	777:778	2D (1H, 1H COSY, 1H/13C HSQC)	777:805	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	5	73	theme	%	1247:1247	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	4	74	dep	Fourier	676:682	arg1	transform					684:692	transform	684:692	transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa	684:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	8	75	theme	polysaccharide	1511:1524	arg1	investigation					1484:1496	pharmacological investigation	1468:1496	pharmacological investigation of extracted polysaccharide	1468:1524	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	3	76	theme	isolation	475:483	arg1	yield					485:489	isolation yield	475:489	isolation yield	475:489	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	4	77	theme	1H/13C	794:799	arg1	1H					781:782	1H	781:782	1H	781:782	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	4	77	theme	1H/13C	794:799	arg1	HSQC					801:804	1H/13C HSQC	794:804	1H/13C HSQC	794:804	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	8	78	theme	central	1605:1611	arg1	activities					1629:1638	central antinociceptive activities	1605:1638	a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities	1535:1638	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	4	79	dep	transform	684:692	arg1	infrared					694:701	infrared	694:701	transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa	684:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	7	80	theme	antioxidant	1429:1439	arg1	effect					1450:1455	antioxidant activity effect	1429:1455	antioxidant activity effect	1429:1455	Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect.
29421049	8	81	theme	anti-inflammatory	1542:1558	arg1	effect					1560:1565	a good anti-inflammatory effect	1535:1565	a good anti-inflammatory effect	1535:1565	Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
29421049	2	82	dep	settings	407:414	arg1	2.9					434:436	2.9	434:436	2.9	434:436	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	82	dep	settings	407:414	arg1	settings					407:414	optimal settings	399:414	optimal settings 90 °C, 37 min and 2.9, respectively	399:450	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	82	dep	settings	407:414	arg1	37 min					423:428	37 min	423:428	37 min	423:428	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	2	82	dep	settings	407:414	arg1	90 °C					416:420	90 °C	416:420	90 °C	416:420	Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively.
29421049	5	83	theme	%	1261:1261	arg1	percentage					1213:1222	the molar percentage	1203:1222	the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively	1203:1285	Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively.
29421049	3	84	theme	antioxidant	501:511	arg1	activity					513:520	total antioxidant activity	495:520	total antioxidant activity	495:520	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	4	85	dep	infrared	694:701	arg1	revealed					866:873	revealed	866:873	revealed that polysaccharide extracted had an average molecular weight of 240 kDa	866:946	Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa.
29421049	3	86	theme	31.8 mg	537:543	arg1	material					572:579	31.8 mg ascorbic acid equivalent/g material	537:579	31.8 mg ascorbic acid equivalent/g material	537:579	Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively.
29421049	1	87	theme	antioxidant	265:275	arg1	ability					277:283	total antioxidant ability	259:283	total antioxidant ability	259:283	Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa.
30658396	0	0	theme	Polysaccharides	86:100	arg1	Modulation					37:46	Immune Modulation	30:46	Immune Modulation	30:46	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	0	theme	Polysaccharides	86:100	arg1	Activities					63:72	Anticancer Activities	52:72	Anticancer Activities	52:72	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	0	theme	Polysaccharides	86:100	arg1	Characterization					8:23	Partial Characterization	0:23	Partial Characterization	0:23	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	10	1	theme	HepG2	1376:1380	arg1	cells					1382:1386	HepG2 cells	1376:1386	HepG2 cells	1376:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	8	2	theme	tumor	939:943	arg1	factor					954:959	tumor necrosis factor α	939:961	tumor necrosis factor α (TNF-α)	939:969	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	8	2	theme	tumor	939:943	arg1	TNF-α					964:968	TNF-α	964:968	TNF-α	964:968	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	9	3	theme	dose-dependent	1013:1026	arg1	activity					1039:1046	significant dose-dependent anticancer activity	1001:1046	significant dose-dependent anticancer activity	1001:1046	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	0	4	theme	Sulfated	77:84	arg1	Polysaccharides					86:100	Sulfated Polysaccharides	77:100	Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp	77:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	9	5	theme	anticancer	1028:1037	arg1	activity					1039:1046	significant dose-dependent anticancer activity	1001:1046	significant dose-dependent anticancer activity	1001:1046	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	0	6	theme	Filamentous	107:117	arg1	sp					140:141	Filamentous Microalgae Tribonema sp	107:141	Filamentous Microalgae Tribonema sp	107:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	11	7	theme	anticancer	1548:1557	arg1	mechanism					1559:1567	the anticancer mechanism	1544:1567	the anticancer mechanism of TSP	1544:1574	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	9	8	from	rate	1068:1071	arg1	µg/mL					1095:1099	250 µg/mL	1091:1099	250 µg/mL	1091:1099	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	9	9	theme	HepG2	1105:1109	arg1	cells					1111:1115	HepG2 cells	1105:1115	HepG2 cells	1105:1115	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	8	10	theme	immune-modulatory	808:824	arg1	activity					826:833	significant immune-modulatory activity	796:833	significant immune-modulatory activity	796:833	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	10	11	dep	cycle	1355:1359	arg1	the					1346:1348	the	1346:1348	the	1346:1348	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	9	12	theme	%	1086:1086	arg1	rate					1068:1071	an inhibition rate	1054:1071	an inhibition rate of up to 66.8% at 250 µg/mL	1054:1099	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	5	13	theme	HepG2	626:630	arg1	cells					632:636	HepG2 cells	626:636	HepG2 cells	626:636	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	10	14	dep	apoptosis	1314:1322	arg1	affecting					1336:1344	affecting	1336:1344	rather than affecting the cell cycle and mitosis of HepG2 cells	1324:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	6	15	theme	kDa	713:715	arg1	Mw					703:704	a Mw	701:704	a Mw of 197 kDa	701:715	The results showed that TSP is a sulfated polysaccharide with a Mw of 197 kDa.
30658396	8	16	theme	significant	796:806	arg1	activity					826:833	significant immune-modulatory activity	796:833	significant immune-modulatory activity	796:833	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	3	17	theme	biological	344:353	arg1	activities					355:364	and their biological activities	334:364	activities	355:364	and their biological activities have rarely been reported.
30658396	10	18	theme	cell	1309:1312	arg1	apoptosis					1314:1322	induced cell apoptosis	1301:1322	induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells	1301:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	0	19	dep	Filamentous	107:117	arg1	Microalgae					119:128	Microalgae	119:128	Microalgae	119:128	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	5	20	theme	chemical	492:499	arg1	composition					501:511	their chemical composition	486:511	their chemical composition	486:511	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	0	21	from	Modulation	37:46	arg1	sp					140:141	Filamentous Microalgae Tribonema sp	107:141	Filamentous Microalgae Tribonema sp	107:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	9	22	dep	66.8	1082:1085	arg1	to					1079:1080	to	1079:1080	to	1079:1080	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	0	23	theme	Tribonema	130:138	arg1	sp					140:141	Filamentous Microalgae Tribonema sp	107:141	Filamentous Microalgae Tribonema sp	107:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	10	24	theme	cell	1350:1353	arg1	cycle					1355:1359	cell cycle	1350:1359	cell cycle	1350:1359	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	2	25	theme	Tribonema	320:328	arg1	sp					330:331	Tribonema sp	320:331	Tribonema sp	320:331	However, the polysaccharides of Tribonema sp.
30658396	9	26	theme	inhibition	1057:1066	arg1	rate					1068:1071	an inhibition rate	1054:1071	an inhibition rate of up to 66.8% at 250 µg/mL	1054:1099	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	1	27	theme	filamentous	179:189	arg1	microalgae					191:200	filamentous microalgae	179:200	filamentous microalgae	179:200	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	0	28	theme	Partial	0:6	arg1	Modulation					37:46	Immune Modulation	30:46	Immune Modulation	30:46	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	28	theme	Partial	0:6	arg1	Activities					63:72	Anticancer Activities	52:72	Anticancer Activities	52:72	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	28	theme	Partial	0:6	arg1	Characterization					8:23	Partial Characterization	0:23	Partial Characterization	0:23	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	1	29	theme	triacylglycerols	270:285	arg1	accumulation					254:265	its accumulation	250:265	its accumulation of triacylglycerols	250:285	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	10	30	theme	cells	1382:1386	arg1	mitosis					1365:1371	mitosis	1365:1371	mitosis	1365:1371	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	10	30	theme	cells	1382:1386	arg1	cycle					1355:1359	cell cycle	1350:1359	cell cycle	1350:1359	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	5	31	theme	RAW264.7	596:603	arg1	cells					616:620	RAW264.7 macrophage cells	596:620	RAW264.7 macrophage cells	596:620	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	9	32	theme	MTT	1200:1202	arg1	assay					1205:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	1	33	theme	microalgae	191:200	arg1	kind					171:174	a kind	169:174	a kind of filamentous microalgae	169:200	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	11	34	theme	further	1473:1479	arg1	research					1481:1488	further research	1473:1488	further research	1473:1488	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	5	35	theme	macrophage	605:614	arg1	cells					616:620	RAW264.7 macrophage cells	596:620	RAW264.7 macrophage cells	596:620	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	1	36	dep	Tribonema	154:162	arg1	sp.					164:166	Tribonema sp., a kind of filamentous microalgae,	154:201	sp.	164:166	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	1	36	dep	Tribonema	154:162	arg1	kind					171:174	a kind	169:174	a kind of filamentous microalgae	169:200	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	10	37	theme	anticancer	1250:1259	arg1	result					1291:1296	the result	1287:1296	the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells	1287:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	10	37	theme	anticancer	1250:1259	arg1	activity					1261:1268	the anticancer activity	1246:1268	the anticancer activity of TSP	1246:1275	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	9	38	theme	-2,5-diphenyltetrazolium	1166:1189	arg1	assay					1205:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	9	39	from	µg/mL	1095:1099	arg1	%					1086:1086	up to 66.8%	1076:1086	up to 66.8% at 250 µg/mL	1076:1099	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	9	39	from	µg/mL	1095:1099	arg1	rate					1068:1071	an inhibition rate	1054:1071	an inhibition rate of up to 66.8% at 250 µg/mL	1054:1099	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	4	40	theme	Tribonema	451:459	arg1	sp					461:462	Tribonema sp	451:462	Tribonema sp	451:462	In this paper, we extracted sulfated polysaccharides from Tribonema sp.
30658396	6	41	theme	sulfated	672:679	arg1	TSP					663:665	TSP	663:665	TSP	663:665	The results showed that TSP is a sulfated polysaccharide with a Mw of 197 kDa.
30658396	6	41	theme	sulfated	672:679	arg1	polysaccharide					681:694	a sulfated polysaccharide	670:694	a sulfated polysaccharide with a Mw of 197 kDa	670:715	The results showed that TSP is a sulfated polysaccharide with a Mw of 197 kDa.
30658396	0	42	theme	Immune	30:35	arg1	Modulation					37:46	Immune Modulation	30:46	Immune Modulation	30:46	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	42	theme	Immune	30:35	arg1	Characterization					8:23	Partial Characterization	0:23	Partial Characterization	0:23	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	9	43	theme	bromide	1191:1197	arg1	assay					1205:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1134:1209	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	10	44	theme	cycle	1216:1220	arg1	analysis					1222:1229	The cycle analysis	1212:1229	The cycle analysis	1212:1229	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	0	45	theme	Anticancer	52:61	arg1	Activities					63:72	Anticancer Activities	52:72	Anticancer Activities	52:72	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	45	theme	Anticancer	52:61	arg1	Characterization					8:23	Partial Characterization	0:23	Partial Characterization	0:23	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	6	46	with	polysaccharide	681:694	arg1	Mw					703:704	a Mw	701:704	a Mw of 197 kDa	701:715	The results showed that TSP is a sulfated polysaccharide with a Mw of 197 kDa.
30658396	0	47	from	Activities	63:72	arg1	sp					140:141	Filamentous Microalgae Tribonema sp	107:141	Filamentous Microalgae Tribonema sp	107:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	48	dep	Modulation	37:46	arg1	the					26:28	the	26:28	the	26:28	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	5	49	theme	immunostimulation	549:565	arg1	activities					582:591	their immunostimulation and anticancer activities	543:591	their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells	543:636	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	4	50	theme	sulfated	421:428	arg1	polysaccharides					430:444	sulfated polysaccharides	421:444	sulfated polysaccharides	421:444	In this paper, we extracted sulfated polysaccharides from Tribonema sp.
30658396	10	51	theme	TSP	1273:1275	arg1	result					1291:1296	the result	1287:1296	the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells	1287:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	10	51	theme	TSP	1273:1275	arg1	activity					1261:1268	the anticancer activity	1246:1268	the anticancer activity of TSP	1246:1275	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	11	52	theme	anticancer	1448:1457	arg1	potential					1432:1440	potential	1432:1440	potential	1432:1440	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	11	52	theme	anticancer	1448:1457	arg1	resource					1459:1466	an anticancer resource	1445:1466	an anticancer resource	1445:1466	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	5	53	theme	anticancer	571:580	arg1	activities					582:591	their immunostimulation and anticancer activities	543:591	their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells	543:636	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	5	54	from	activities	582:591	arg1	cells					616:620	RAW264.7 macrophage cells	596:620	RAW264.7 macrophage cells	596:620	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	5	54	from	activities	582:591	arg1	cells					632:636	HepG2 cells	626:636	HepG2 cells	626:636	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	11	55	theme	TSP	1572:1574	arg1	mechanism					1559:1567	the anticancer mechanism	1544:1567	the anticancer mechanism of TSP	1544:1574	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	10	56	theme	induced	1301:1307	arg1	apoptosis					1314:1322	induced cell apoptosis	1301:1322	induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells	1301:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	2	57	theme	sp	330:331	arg1	polysaccharides					301:315	the polysaccharides	297:315	the polysaccharides of Tribonema sp	297:331	However, the polysaccharides of Tribonema sp.
30658396	8	58	theme	macrophage	850:859	arg1	cells					861:865	macrophage cells	850:865	macrophage cells	850:865	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	1	59	theme	biofuel	224:230	arg1	production					232:241	biofuel production	224:241	biofuel production due to its accumulation of triacylglycerols	224:285	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30658396	11	60	contain	have	1427:1430	arg1	TSP					1417:1419	TSP	1417:1419	TSP	1417:1419	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	11	60	contain	have	1427:1430	arg2	potential					1432:1440	potential	1432:1440	potential	1432:1440	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	11	60	contain	have	1427:1430	arg2	resource					1459:1466	an anticancer resource	1445:1466	an anticancer resource	1445:1466	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	5	61	dep	characterized	472:484	arg1	TSP					466:468	TSP	466:468	TSP	466:468	(TSP), characterized their chemical composition and structure, and determined their immunostimulation and anticancer activities on RAW264.7 macrophage cells and HepG2 cells.
30658396	10	62	theme	apoptosis	1314:1322	arg1	result					1291:1296	the result	1287:1296	the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells	1287:1386	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	10	62	theme	apoptosis	1314:1322	arg1	activity					1261:1268	the anticancer activity	1246:1268	the anticancer activity of TSP	1246:1275	The cycle analysis indicated that the anticancer activity of TSP is mainly the result of induced cell apoptosis rather than affecting the cell cycle and mitosis of HepG2 cells.
30658396	11	63	theme	vivo	1515:1518	arg1	experiments					1520:1530	vivo experiments	1515:1530	vivo experiments	1515:1530	These findings suggest that TSP might have potential as an anticancer resource, but further research is needed, especially in vivo experiments, to explore the anticancer mechanism of TSP.
30658396	0	64	from	sp	140:141	arg1	Modulation					37:46	Immune Modulation	30:46	Immune Modulation	30:46	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	64	from	sp	140:141	arg1	Activities					63:72	Anticancer Activities	52:72	Anticancer Activities	52:72	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	64	from	sp	140:141	arg1	Polysaccharides					86:100	Sulfated Polysaccharides	77:100	Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp	77:141	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	0	64	from	sp	140:141	arg1	Characterization					8:23	Partial Characterization	0:23	Partial Characterization	0:23	Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.
30658396	8	65	theme	necrosis	945:952	arg1	factor					954:959	tumor necrosis factor α	939:961	tumor necrosis factor α (TNF-α)	939:969	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	8	65	theme	necrosis	945:952	arg1	TNF-α					964:968	TNF-α	964:968	TNF-α	964:968	It showed significant immune-modulatory activity by stimulating macrophage cells, such as upregulating interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor α (TNF-α).
30658396	9	66	theme	significant	1001:1011	arg1	activity					1039:1046	significant dose-dependent anticancer activity	1001:1046	significant dose-dependent anticancer activity	1001:1046	In addition, TSP also showed significant dose-dependent anticancer activity (with an inhibition rate of up to 66.8% at 250 µg/mL) on HepG2 cells as determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
30658396	1	67	theme	due	243:245	arg1	production					232:241	biofuel production	224:241	biofuel production due to its accumulation of triacylglycerols	224:285	Recently, Tribonema sp., a kind of filamentous microalgae, has been studied for biofuel production due to its accumulation of triacylglycerols.
30977470	0	0	theme	wound	110:114	arg1	materials					125:133	wound dressing materials	110:133	wound dressing materials	110:133	Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials.
30977470	7	1	theme	animal	1191:1196	arg1	tests					1198:1202	The in vivo animal tests	1179:1202	The in vivo animal tests	1179:1202	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	1	2	theme	mortality	173:181	arg1	injury					146:151	Traumatic injury	136:151	Traumatic injury	136:151	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	1	2	theme	mortality	173:181	arg1	cause					164:168	a major cause	156:168	a major cause of mortality	156:181	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	5	3	theme	interlayer	728:737	arg1	space					739:743	the interlayer space	724:743	the interlayer space of the organic rectorite (OREC)	724:775	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	5	4	theme	improved	852:859	arg1	activity					875:882	improved antimicrobial activity	852:882	improved antimicrobial activity	852:882	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	7	5	theme	in	1183:1184	arg1	tests					1198:1202	The in vivo animal tests	1179:1202	The in vivo animal tests	1179:1202	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	7	6	theme	hemostatic	1342:1351	arg1	materials					1353:1361	the traditional hemostatic materials	1326:1361	the traditional hemostatic materials	1326:1361	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	0	7	theme	dressing	116:123	arg1	materials					125:133	wound dressing materials	110:133	wound dressing materials	110:133	Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials.
30977470	8	8	dep	healing	1487:1493	arg1	days					1497:1500	3 days	1495:1500	wound healing 3 days earlier than gauze	1481:1519	Furthermore, BACNFs/QCRs could substantially promote collagen synthesis and neovascularization, thereby accelerating wound healing 3 days earlier than gauze.
30977470	7	9	theme	BACNF/QCR	1226:1234	arg1	sponges					1236:1242	the BACNF/QCR sponges	1222:1242	the BACNF/QCR sponges	1222:1242	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	3	10	with	suspension	372:381	arg1	ratio					460:464	a high aspect ratio	446:464	a high aspect ratio	446:464	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	6	11	theme	BACNF	932:936	arg1	sponge					938:943	the BACNF sponge	928:943	the BACNF sponge	928:943	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	6	12	theme	composite	1113:1121	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	6	13	theme	hydrogen	1028:1035	arg1	bonding					1037:1043	hydrogen bonding	1028:1043	hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying	1028:1176	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	8	14	theme	wound	1481:1485	arg1	healing					1487:1493	wound healing 3 days earlier than gauze	1481:1519	wound healing 3 days earlier than gauze	1481:1519	Furthermore, BACNFs/QCRs could substantially promote collagen synthesis and neovascularization, thereby accelerating wound healing 3 days earlier than gauze.
30977470	4	15	theme	wound	617:621	arg1	dressing					623:630	a wound dressing	615:630	a wound dressing	615:630	The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing.
30977470	5	16	theme	organic	752:758	arg1	OREC					771:774	OREC	771:774	OREC	771:774	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	5	16	theme	organic	752:758	arg1	rectorite					760:768	the organic rectorite	748:768	the organic rectorite (OREC)	748:775	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	3	17	theme	cellulose	411:419	arg1	BACNFs					433:438	BACNFs	433:438	BACNFs	433:438	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	17	theme	cellulose	411:419	arg1	nanofibers					421:430	carboxylated brown algae cellulose nanofibers	386:430	carboxylated brown algae cellulose nanofibers (BACNFs)	386:439	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	9	18	theme	wound	1639:1643	arg1	healing					1645:1651	wound healing	1639:1651	wound healing	1639:1651	This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.
30977470	2	19	theme	dressings	276:284	arg1	essential					289:297	essential	289:297	essential	289:297	Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality.
30977470	2	19	theme	dressings	276:284	arg1	development					245:255	the development	241:255	the development of effective wound dressings	241:284	Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality.
30977470	1	20	theme	poor	188:191	arg1	healing					199:205	poor wound healing	188:205	poor wound healing	188:205	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	5	21	theme	rectorite	760:768	arg1	space					739:743	the interlayer space	724:743	the interlayer space of the organic rectorite (OREC)	724:775	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	8	22	theme	collagen	1417:1424	arg1	synthesis					1426:1434	collagen synthesis	1417:1434	collagen synthesis	1417:1434	Furthermore, BACNFs/QCRs could substantially promote collagen synthesis and neovascularization, thereby accelerating wound healing 3 days earlier than gauze.
30977470	1	23	theme	wound	193:197	arg1	healing					199:205	poor wound healing	188:205	poor wound healing	188:205	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	3	24	theme	carboxylated	386:397	arg1	BACNFs					433:438	BACNFs	433:438	BACNFs	433:438	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	24	theme	carboxylated	386:397	arg1	nanofibers					421:430	carboxylated brown algae cellulose nanofibers	386:430	carboxylated brown algae cellulose nanofibers (BACNFs)	386:439	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	25	from	dried	477:481	arg1	study					363:367	this study	358:367	this study	358:367	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	2	26	theme	wound	315:319	arg1	healing					321:327	wound healing	315:327	wound healing	315:327	Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality.
30977470	6	27	theme	electrostatic	997:1009	arg1	interactions					1011:1022	electrostatic interactions	997:1022	electrostatic interactions	997:1022	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	6	28	theme	BACNF/QCR	1124:1132	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	3	29	theme	aspect	453:458	arg1	ratio					460:464	a high aspect ratio	446:464	a high aspect ratio	446:464	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	0	30	theme	cellulose	16:24	arg1	Construction					0:11	Construction	0:11	Construction of cellulose	0:24	Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials.
30977470	4	31	theme	absorption	546:555	arg1	capacity					557:564	water absorption capacity	540:564	water absorption capacity	540:564	The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing.
30977470	9	32	theme	natural	1582:1588	arg1	substances					1590:1599	natural substances	1582:1599	natural substances	1582:1599	This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.
30977470	1	33	theme	Traumatic	136:144	arg1	injury					146:151	Traumatic injury	136:151	Traumatic injury	136:151	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	1	33	theme	Traumatic	136:144	arg1	cause					164:168	a major cause	156:168	a major cause of mortality	156:181	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	5	34	theme	electrostatic	781:793	arg1	interactions					795:806	electrostatic interactions	781:806	electrostatic interactions	781:806	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	0	35	theme	rectorite	64:72	arg1	composites					74:83	chitin/organic rectorite composites	49:83	chitin/organic rectorite composites	49:83	Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials.
30977470	4	36	theme	water	540:544	arg1	capacity					557:564	water absorption capacity	540:564	water absorption capacity	540:564	The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing.
30977470	7	37	theme	rat	1276:1278	arg1	test					1296:1299	a rat tail amputation test	1274:1299	a rat tail amputation test	1274:1299	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	7	38	theme	tail	1280:1283	arg1	test					1296:1299	a rat tail amputation test	1274:1299	a rat tail amputation test	1274:1299	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	5	39	with	β-chitin	658:665	arg1	properties					691:700	antibacterial properties	677:700	antibacterial properties	677:700	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	0	40	theme	chitin/organic	49:62	arg1	composites					74:83	chitin/organic rectorite composites	49:83	chitin/organic rectorite composites	49:83	Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials.
30977470	3	41	theme	nanofibers	421:430	arg1	dried					477:481	dried	477:481	dried	477:481	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	41	theme	nanofibers	421:430	arg1	suspension					372:381	a suspension	370:381	a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio	370:464	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	42	from	study	363:367	arg1	dried					477:481	dried	477:481	dried	477:481	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	42	from	study	363:367	arg1	suspension					372:381	a suspension	370:381	a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio	370:464	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	43	theme	high	448:451	arg1	ratio					460:464	a high aspect ratio	446:464	a high aspect ratio	446:464	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	44	theme	brown	399:403	arg1	BACNFs					433:438	BACNFs	433:438	BACNFs	433:438	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	44	theme	brown	399:403	arg1	nanofibers					421:430	carboxylated brown algae cellulose nanofibers	386:430	carboxylated brown algae cellulose nanofibers (BACNFs)	386:439	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	5	45	theme	antimicrobial	861:873	arg1	activity					875:882	improved antimicrobial activity	852:882	improved antimicrobial activity	852:882	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	3	46	theme	algae	405:409	arg1	BACNFs					433:438	BACNFs	433:438	BACNFs	433:438	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	3	46	theme	algae	405:409	arg1	nanofibers					421:430	carboxylated brown algae cellulose nanofibers	386:430	carboxylated brown algae cellulose nanofibers (BACNFs)	386:439	In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge.
30977470	4	47	theme	high	522:525	arg1	porosity					527:534	high porosity	522:534	high porosity	522:534	The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing.
30977470	9	48	theme	multi-functional	1527:1542	arg1	material					1555:1562	This multi-functional biomedical material	1522:1562	This multi-functional biomedical material	1522:1562	This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.
30977470	6	49	theme	rectorite	1103:1111	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	7	50	dep	in	1183:1184	arg1	vivo					1186:1189	vivo	1186:1189	vivo	1186:1189	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	7	51	theme	traditional	1330:1340	arg1	materials					1353:1361	the traditional hemostatic materials	1326:1361	the traditional hemostatic materials	1326:1361	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	2	52	theme	wound	270:274	arg1	dressings					276:284	effective wound dressings	260:284	effective wound dressings	260:284	Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality.
30977470	6	53	theme	chitin/organic	1088:1101	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	6	54	theme	nanofiber/quaternized	1066:1086	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	5	55	theme	quaternized	646:656	arg1	QC					668:669	QC	668:669	QC	668:669	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	5	55	theme	quaternized	646:656	arg1	β-chitin					658:665	quaternized β-chitin	646:665	quaternized β-chitin (QC) with antibacterial properties	646:700	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	8	56	theme	earlier	1502:1508	arg1	days					1497:1500	3 days	1495:1500	wound healing 3 days earlier than gauze	1481:1519	Furthermore, BACNFs/QCRs could substantially promote collagen synthesis and neovascularization, thereby accelerating wound healing 3 days earlier than gauze.
30977470	2	57	theme	effective	260:268	arg1	dressings					276:284	effective wound dressings	260:284	effective wound dressings	260:284	Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality.
30977470	1	58	theme	major	158:162	arg1	injury					146:151	Traumatic injury	136:151	Traumatic injury	136:151	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	1	58	theme	major	158:162	arg1	cause					164:168	a major cause	156:168	a major cause of mortality	156:181	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	6	59	theme	QCR	963:965	arg1	suspension					967:976	the QCR suspension	959:976	the QCR suspension	959:976	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	1	60	theme	people	227:232	arg1	millions					215:222	millions	215:222	millions of people	215:232	Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people.
30977470	7	61	theme	amputation	1285:1294	arg1	test					1296:1299	a rat tail amputation test	1274:1299	a rat tail amputation test	1274:1299	The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials.
30977470	6	62	theme	cellulose	1056:1064	arg1	sponges					1135:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges	1056:1141	Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying.
30977470	5	63	theme	composite	818:826	arg1	QCRs					841:844	QCRs	841:844	QCRs	841:844	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	5	63	theme	composite	818:826	arg1	suspensions					828:838	composite suspensions	818:838	composite suspensions (QCRs)	818:845	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
30977470	4	64	theme	wound	587:591	arg1	exudates					593:600	wound exudates	587:600	wound exudates	587:600	The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing.
30977470	9	65	theme	great	1608:1612	arg1	potential					1614:1622	great potential	1608:1622	great potential to be used for wound healing	1608:1651	This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.
30977470	9	66	theme	biomedical	1544:1553	arg1	material					1555:1562	This multi-functional biomedical material	1522:1562	This multi-functional biomedical material	1522:1562	This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.
30977470	5	67	theme	antibacterial	677:689	arg1	properties					691:700	antibacterial properties	677:700	antibacterial properties	677:700	In addition, quaternized β-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone.
31176860	0	0	theme	chitosan	83:90	arg1	morphology					51:60	surface morphology	43:60	surface morphology	43:60	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	0	0	theme	chitosan	83:90	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	0	0	theme	chitosan	83:90	arg1	features					33:40	structural features	22:40	structural features	22:40	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	0	0	theme	chitosan	83:90	arg1	bioactivities					66:78	bioactivities	66:78	bioactivities	66:78	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	2	1	theme	high	430:433	arg1	level					435:439	high level	430:439	high level (590 ppm)	430:449	Chitin and chitosan was extracted by chemical methods and yield was recorded 35% and 41%, moisture and ash has showed 7.4, 0.73, 1.2 and 0.79% and Ca was recorded high level (590 ppm).
31176860	2	1	theme	high	430:433	arg1	590 ppm					442:448	590 ppm	442:448	590 ppm	442:448	Chitin and chitosan was extracted by chemical methods and yield was recorded 35% and 41%, moisture and ash has showed 7.4, 0.73, 1.2 and 0.79% and Ca was recorded high level (590 ppm).
31176860	7	2	theme	β-glucosidase	956:968	arg1	effect					988:993	The α-amylase and β-glucosidase enzyme inhibition effect	938:993	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan	938:1005	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	1	3	theme	chemical	151:158	arg1	composition					160:170	The chemical composition	147:170	The chemical composition	147:170	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	7	4	theme	enzyme	970:975	arg1	effect					988:993	The α-amylase and β-glucosidase enzyme inhibition effect	938:993	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan	938:1005	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	4	5	theme	glucosamine	596:606	arg1	proton					586:591	H-2 proton	582:591	H-2 proton of glucosamine (GlcN)	582:613	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	5	theme	glucosamine	596:606	arg1	protons					625:631	Anomeric protons	616:631	Anomeric protons	616:631	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	7	6	theme	α-amylase	942:950	arg1	effect					988:993	The α-amylase and β-glucosidase enzyme inhibition effect	938:993	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan	938:1005	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	3	7	theme	primary	482:488	arg1	amines					504:509	NH primary and secondary amines	479:509	NH primary and secondary amines	479:509	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	7	theme	primary	482:488	arg1	group					515:519	OH group	512:519	OH group	512:519	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	7	theme	primary	482:488	arg1	stretching					539:548	several sugar stretching	525:548	several sugar stretching	525:548	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	7	8	theme	anticoagulant	1049:1061	arg1	potential					1063:1071	anticoagulant potential	1049:1071	anticoagulant potential	1049:1071	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	4	9	theme	carbon	711:716	arg1	atoms					718:722	anomeric, methyl carbon atoms	694:722	atoms	718:722	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	3	10	theme	IR	456:457	arg1	spectrum					459:466	The IR spectrum	452:466	The IR spectrum has	452:470	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	8	11	theme	pharmaceutical	1100:1113	arg1	industry					1115:1122	pharmaceutical industry	1100:1122	pharmaceutical industry	1100:1122	Chitosan could be used in pharmaceutical industry and tissue engineering.
31176860	0	12	theme	lobster	109:115	arg1	shells					139:144	lobster (Thenus unimaculatus) shells	109:144	lobster (Thenus unimaculatus) shells	109:144	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	4	13	from	NMR	686:688	arg1	NMR					731:733	13C NMR	727:733	13C NMR	727:733	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	14	theme	1H	683:684	arg1	NMR					686:688	1H NMR	683:688	1H NMR	683:688	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	15	dep	proton	586:591	arg1	H-1D					645:648	H-1D	645:648	H-1D	645:648	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	15	dep	proton	586:591	arg1	GlcNAc					652:657	GlcNAc	652:657	GlcNAc (H-1A)	652:664	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	15	dep	proton	586:591	arg1	[GlcN					638:642	H-1 [GlcN	634:642	H-1 [GlcN (H-1D)	634:649	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	6	16	theme	antioxidant	918:928	arg1	assays					930:935	four antioxidant assays	913:935	four antioxidant assays	913:935	Antioxidant effect has showed good and concentration depended in four antioxidant assays.
31176860	1	17	theme	structural	173:182	arg1	features					184:191	structural features	173:191	structural features	173:191	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	5	18	with	crystalline	768:778	arg1	cluster					785:791	cluster	785:791	cluster	785:791	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	4	19	theme	anomeric	694:701	arg1	atoms					718:722	anomeric, methyl carbon atoms	694:722	atoms	718:722	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	20	theme	H-2	582:584	arg1	proton					586:591	H-2 proton	582:591	H-2 proton of glucosamine (GlcN)	582:613	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	20	theme	H-2	582:584	arg1	protons					625:631	Anomeric protons	616:631	Anomeric protons	616:631	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	0	21	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	7	22	theme	chitosan	998:1005	arg1	effect					988:993	The α-amylase and β-glucosidase enzyme inhibition effect	938:993	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan	938:1005	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	5	23	with	surface	742:748	arg1	cluster					785:791	cluster	785:791	cluster	785:791	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	3	24	theme	secondary	494:502	arg1	amines					504:509	NH primary and secondary amines	479:509	NH primary and secondary amines	479:509	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	24	theme	secondary	494:502	arg1	group					515:519	OH group	512:519	OH group	512:519	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	24	theme	secondary	494:502	arg1	stretching					539:548	several sugar stretching	525:548	several sugar stretching	525:548	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	0	25	theme	structural	22:31	arg1	features					33:40	structural features	22:40	structural features	22:40	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	2	26	dep	%	346:346	arg1	has					374:376	has	374:376	has	374:376	Chitin and chitosan was extracted by chemical methods and yield was recorded 35% and 41%, moisture and ash has showed 7.4, 0.73, 1.2 and 0.79% and Ca was recorded high level (590 ppm).
31176860	4	27	theme	N-acetyl	551:558	arg1	glucosamine					560:570	N-acetyl glucosamine	551:570	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)]	551:665	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	27	theme	N-acetyl	551:558	arg1	GlcNAc					573:578	GlcNAc	573:578	GlcNAc	573:578	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	5	28	theme	porosity	797:804	arg1	structure					806:814	porosity structure	797:814	porosity structure	797:814	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	2	29	theme	chemical	304:311	arg1	methods					313:319	chemical methods	304:319	chemical methods	304:319	Chitin and chitosan was extracted by chemical methods and yield was recorded 35% and 41%, moisture and ash has showed 7.4, 0.73, 1.2 and 0.79% and Ca was recorded high level (590 ppm).
31176860	4	30	dep	atoms	718:722	arg1	methyl					704:709	anomeric, methyl carbon atoms	694:722	methyl	704:709	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	3	31	theme	OH	512:513	arg1	amines					504:509	NH primary and secondary amines	479:509	NH primary and secondary amines	479:509	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	31	theme	OH	512:513	arg1	group					515:519	OH group	512:519	OH group	512:519	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	1	32	theme	chitosan	214:221	arg1	derivatives					223:233	chitosan derivatives	214:233	chitosan derivatives	214:233	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	5	33	with	structure	806:814	arg1	cluster					785:791	cluster	785:791	cluster	785:791	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	5	34	with	block	761:765	arg1	cluster					785:791	cluster	785:791	cluster	785:791	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	4	35	dep	glucosamine	560:570	arg1	proton					586:591	H-2 proton	582:591	H-2 proton of glucosamine (GlcN)	582:613	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	35	dep	glucosamine	560:570	arg1	protons					625:631	Anomeric protons	616:631	Anomeric protons	616:631	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	1	36	theme	derivatives	223:233	arg1	features					184:191	structural features	173:191	structural features	173:191	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	1	36	theme	derivatives	223:233	arg1	bioactivities					197:209	bioactivities	197:209	bioactivities	197:209	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	1	36	theme	derivatives	223:233	arg1	composition					160:170	The chemical composition	147:170	The chemical composition	147:170	The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here.
31176860	7	37	theme	inhibition	977:986	arg1	effect					988:993	The α-amylase and β-glucosidase enzyme inhibition effect	938:993	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan	938:1005	The α-amylase and β-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential.
31176860	4	38	theme	13C	727:729	arg1	NMR					731:733	13C NMR	727:733	13C NMR	727:733	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	6	39	dep	effect	860:865	arg1	has					867:869	has	867:869	has	867:869	Antioxidant effect has showed good and concentration depended in four antioxidant assays.
31176860	5	40	theme	Rough	736:740	arg1	surface					742:748	Rough surface	736:748	Rough surface	736:748	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	0	41	theme	surface	43:49	arg1	morphology					51:60	surface morphology	43:60	surface morphology	43:60	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	3	42	dep	spectrum	459:466	arg1	has					468:470	has	468:470	has	468:470	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	5	43	theme	SEM	831:833	arg1	observation					835:845	SEM observation	831:845	SEM observation	831:845	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	3	44	theme	sugar	533:537	arg1	amines					504:509	NH primary and secondary amines	479:509	NH primary and secondary amines	479:509	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	44	theme	sugar	533:537	arg1	stretching					539:548	several sugar stretching	525:548	several sugar stretching	525:548	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	4	45	theme	Anomeric	616:623	arg1	protons					625:631	Anomeric protons	616:631	Anomeric protons	616:631	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	45	theme	Anomeric	616:623	arg1	proton					586:591	H-2 proton	582:591	H-2 proton of glucosamine (GlcN)	582:613	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	0	46	from	shells	139:144	arg1	derivatives					92:102	derivatives	92:102	derivatives	92:102	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	8	47	used	used	1092:1095	arg2	Chitosan					1074:1081	Chitosan	1074:1081	Chitosan	1074:1081	Chitosan could be used in pharmaceutical industry and tissue engineering.
31176860	4	48	theme	H-1	634:636	arg1	H-1D					645:648	H-1D	645:648	H-1D	645:648	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	48	theme	H-1	634:636	arg1	GlcNAc					652:657	GlcNAc	652:657	GlcNAc (H-1A)	652:664	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	4	48	theme	H-1	634:636	arg1	[GlcN					638:642	H-1 [GlcN	634:642	H-1 [GlcN (H-1D)	634:649	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	5	49	theme	irregular	751:759	arg1	block					761:765	irregular block	751:765	irregular block	751:765	Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation.
31176860	6	50	theme	Antioxidant	848:858	arg1	effect					860:865	Antioxidant effect has	848:869	Antioxidant effect has	848:869	Antioxidant effect has showed good and concentration depended in four antioxidant assays.
31176860	8	51	theme	tissue	1128:1133	arg1	engineering					1135:1145	tissue engineering	1128:1145	tissue engineering	1128:1145	Chitosan could be used in pharmaceutical industry and tissue engineering.
31176860	0	52	dep	shells	139:144	arg1	unimaculatus					125:136	Thenus unimaculatus	118:136	Thenus unimaculatus	118:136	Chemical composition, structural features, surface morphology and bioactivities of chitosan derivatives from lobster (Thenus unimaculatus) shells.
31176860	4	53	from	atoms	718:722	arg1	NMR					731:733	13C NMR	727:733	13C NMR	727:733	N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR.
31176860	3	54	theme	several	525:531	arg1	amines					504:509	NH primary and secondary amines	479:509	NH primary and secondary amines	479:509	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
31176860	3	54	theme	several	525:531	arg1	stretching					539:548	several sugar stretching	525:548	several sugar stretching	525:548	The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching.
30871432	8	0	theme	control	1130:1136	arg1	mice					1142:1145	control fed mice	1130:1145	control fed mice	1130:1145	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	13	1	from	composition	2030:2040	arg1	mice					2107:2110	healthy male BALB/c mice	2087:2110	healthy male BALB/c mice	2087:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	1	2	from	development	226:236	arg1	essential					207:215	essential	207:215	essential	207:215	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	1	2	from	development	226:236	arg1	microbiota					174:183	The intestinal microbiota	159:183	The intestinal microbiota	159:183	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	4	3	from	development	706:716	arg1	mice					721:724	mice	721:724	mice	721:724	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	4	4	theme	intestinal	684:693	arg1	development					706:716	intestinal microbiota development	684:716	intestinal microbiota development	684:716	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	9	5	theme	scGOS	1382:1386	arg1	lcFOS					1388:1392	the scGOS:lcFOS	1378:1392	the scGOS:lcFOS	1378:1392	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	8	6	theme	scGOS	1148:1152	arg1	mice					1164:1167	scGOS:lcFOS fed mice	1148:1167	scGOS:lcFOS fed mice	1148:1167	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	4	7	from	behaviour	670:678	arg1	mice					721:724	mice	721:724	mice	721:724	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	8	8	theme	lcFOS	1154:1158	arg1	mice					1164:1167	scGOS:lcFOS fed mice	1148:1167	scGOS:lcFOS fed mice	1148:1167	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	4	9	theme	onwards	659:665	arg1	day					646:648	the day	642:648	the day of birth onwards	642:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	10	10	theme	fed	1495:1497	arg1	mice					1499:1502	scGOS:lcFOS fed mice	1483:1502	scGOS:lcFOS fed mice	1483:1502	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	5	11	theme	Healthy	735:741	arg1	mice					758:761	Healthy male BALB/cByJ mice	735:761	Healthy male BALB/cByJ mice	735:761	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	2	12	theme	short-chain	310:320	arg1	galacto-oligosaccharides					322:345	short-chain galacto-oligosaccharides	310:345	short-chain galacto-oligosaccharides (scGOS)	310:353	Their composition can be modulated by prebiotics such as short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharide (lcFOS).
30871432	2	12	theme	short-chain	310:320	arg1	scGOS					348:352	scGOS	348:352	scGOS	348:352	Their composition can be modulated by prebiotics such as short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharide (lcFOS).
30871432	5	13	theme	BALB/cByJ	748:756	arg1	mice					758:761	Healthy male BALB/cByJ mice	735:761	Healthy male BALB/cByJ mice	735:761	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	5	14	theme	3	829:829	arg1	%					830:830	%	830:830	%	830:830	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	7	15	theme	short-chain	1005:1015	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	8	16	theme	reduced	1176:1182	arg1	behaviour					1212:1220	reduced anxiety-like and repetitive behaviour	1176:1220	reduced anxiety-like and repetitive behaviour	1176:1220	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	1	17	from	essential	207:215	arg1	behaviour					242:250	behaviour	242:250	behaviour	242:250	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	1	17	from	essential	207:215	arg1	development					226:236	brain development	220:236	brain development	220:236	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	6	18	theme	early	913:917	arg1	adulthood					919:927	early adulthood	913:927	early adulthood	913:927	Behavioural tests were performed pre-weaning, in adolescence, early adulthood and adulthood.
30871432	1	19	from	behaviour	242:250	arg1	essential					207:215	essential	207:215	essential	207:215	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	1	19	from	behaviour	242:250	arg1	microbiota					174:183	The intestinal microbiota	159:183	The intestinal microbiota	159:183	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	14	20	theme	several	2204:2210	arg1	stages					2226:2231	several developmental stages	2204:2231	several developmental stages throughout life	2204:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	4	21	from	day	646:648	arg1	mixture					629:635	this specific mixture	615:635	this specific mixture from the day of birth onwards	615:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	5	22	theme	%	830:830	arg1	scGOS					832:836	3% scGOS	829:836	3% scGOS:lcFOS (9:1)	829:848	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	7	23	theme	microbiota	963:972	arg1	compositions					974:985	faecal microbiota compositions	956:985	faecal microbiota compositions	956:985	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	13	24	from	day	1878:1880	arg1	lcFOS					1863:1867	lcFOS	1863:1867	lcFOS from the day of birth	1863:1889	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	11	25	theme	control	1671:1677	arg1	mice					1683:1686	the control fed mice	1667:1686	the control fed mice	1667:1686	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	0	26	from	Supplementation	0:14	arg1	onwards					70:76	birth onwards	64:76	birth onwards	64:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	14	27	theme	lcFOS	2182:2186	arg1	impact					2166:2171	the potential impact	2152:2171	the potential impact of scGOS:lcFOS on behaviour	2152:2199	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	0	28	theme	anxiety-like	104:115	arg1	behaviour					117:125	anxiety-like behaviour	104:125	anxiety-like behaviour in male BALB/c mice	104:145	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	13	29	theme	improved	1935:1942	arg1	behaviour					1951:1959	reduced anxiety-like and improved social behaviour	1910:1959	reduced anxiety-like and improved social behaviour during the developmental period and later in life	1910:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	4	30	theme	scGOS	508:512	arg1	mixture					497:503	the prebiotic mixture	483:503	the prebiotic mixture of scGOS and lcFOS	483:522	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	14	31	theme	scGOS	2176:2180	arg1	lcFOS					2182:2186	scGOS:lcFOS	2176:2186	scGOS:lcFOS	2176:2186	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	9	32	theme	serotonergic	1280:1291	arg1	system					1293:1298	The serotonergic system	1276:1298	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC)	1276:1360	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	14	33	from	impact	2166:2171	arg1	behaviour					2191:2199	behaviour	2191:2199	behaviour	2191:2199	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	4	34	theme	lcFOS	518:522	arg1	mixture					497:503	the prebiotic mixture	483:503	the prebiotic mixture of scGOS and lcFOS	483:522	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	14	35	theme	impact	2166:2171	arg1	evidence					2140:2147	further evidence	2132:2147	further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life	2132:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	5	36	theme	male	743:746	arg1	mice					758:761	Healthy male BALB/cByJ mice	735:761	Healthy male BALB/cByJ mice	735:761	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	3	37	theme	Several	402:408	arg1	studies					410:416	Several studies	402:416	Several studies	402:416	Several studies reported potential health benefit of prebiotics on behaviour.
30871432	10	38	link	brain-derived	1426:1438	arg1	Bdnf					1461:1464	Bdnf	1461:1464	Bdnf	1461:1464	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	10	38	link	brain-derived	1426:1438	arg1	factor					1453:1458	brain-derived neurotrophic factor	1426:1458	brain-derived neurotrophic factor (Bdnf)	1426:1465	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	0	39	theme	dietary	19:25	arg1	oligosaccharides					42:57	dietary non-digestible oligosaccharides	19:57	dietary non-digestible oligosaccharides from birth onwards	19:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	9	40	theme	prefrontal	1307:1316	arg1	PFC					1326:1328	PFC	1326:1328	PFC	1326:1328	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	9	40	theme	prefrontal	1307:1316	arg1	cortex					1318:1323	the prefrontal cortex	1303:1323	the prefrontal cortex (PFC)	1303:1329	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	12	41	theme	fermentation	1762:1773	arg1	activity					1775:1782	decreased proteolytic fermentation activity	1740:1782	decreased proteolytic fermentation activity	1740:1782	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	6	42	theme	Behavioural	851:861	arg1	tests					863:867	Behavioural tests	851:867	Behavioural tests	851:867	Behavioural tests were performed pre-weaning, in adolescence, early adulthood and adulthood.
30871432	14	43	from	evidence	2140:2147	arg1	behaviour					2191:2199	behaviour	2191:2199	behaviour	2191:2199	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	4	44	theme	dietary	586:592	arg1	supplementation					594:608	dietary supplementation	586:608	dietary supplementation with this specific mixture from the day of birth onwards	586:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	10	45	theme	mRNA	1407:1410	arg1	expression					1412:1421	mRNA expression	1407:1421	mRNA expression of brain-derived neurotrophic factor (Bdnf)	1407:1465	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	0	46	theme	oligosaccharides	42:57	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of dietary non-digestible oligosaccharides from birth onwards	0:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	13	47	theme	male	2095:2098	arg1	mice					2107:2110	healthy male BALB/c mice	2087:2110	healthy male BALB/c mice	2087:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	5	48	theme	dietary	794:800	arg1	supplement					802:811	a dietary supplement	792:811	a dietary supplement with or without 3% scGOS:lcFOS (9:1)	792:848	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	3	49	theme	health	437:442	arg1	benefit					444:450	potential health benefit	427:450	potential health benefit of prebiotics on behaviour	427:477	Several studies reported potential health benefit of prebiotics on behaviour.
30871432	11	50	theme	lcFOS	1595:1599	arg1	diet					1601:1604	the scGOS:lcFOS diet	1585:1604	the scGOS:lcFOS diet	1585:1604	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	13	51	theme	reduced	1910:1916	arg1	behaviour					1951:1959	reduced anxiety-like and improved social behaviour	1910:1959	reduced anxiety-like and improved social behaviour during the developmental period and later in life	1910:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	0	52	theme	birth	64:68	arg1	onwards					70:76	birth onwards	64:76	birth onwards	64:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	12	53	theme	increased	1712:1720	arg1	saccharolytic					1722:1734	an increased saccharolytic	1709:1734	an increased saccharolytic	1709:1734	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	10	54	theme	neurotrophic	1440:1451	arg1	Bdnf					1461:1464	Bdnf	1461:1464	Bdnf	1461:1464	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	10	54	theme	neurotrophic	1440:1451	arg1	factor					1453:1458	brain-derived neurotrophic factor	1426:1458	brain-derived neurotrophic factor (Bdnf)	1426:1465	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	9	55	theme	somatosensory	1335:1347	arg1	SSC					1357:1359	SSC	1357:1359	SSC	1357:1359	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	9	55	theme	somatosensory	1335:1347	arg1	cortex					1349:1354	somatosensory cortex	1335:1354	somatosensory cortex (SSC)	1335:1360	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	12	56	theme	decreased	1740:1748	arg1	activity					1775:1782	decreased proteolytic fermentation activity	1740:1782	decreased proteolytic fermentation activity	1740:1782	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	14	57	from	stages	2226:2231	arg1	evidence					2140:2147	further evidence	2132:2147	further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life	2132:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	13	58	dep	composition	2030:2040	arg1	the					2026:2028	the	2026:2028	the	2026:2028	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	14	59	theme	further	2132:2138	arg1	evidence					2140:2147	further evidence	2132:2147	further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life	2132:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	5	60	dep	scGOS	832:836	arg1	lcFOS					838:842	lcFOS	838:842	3% scGOS:lcFOS (9:1)	829:848	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	5	60	dep	scGOS	832:836	arg1	9:1					845:847	9:1	845:847	9:1	845:847	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	13	61	theme	birth	1885:1889	arg1	day					1878:1880	the day	1874:1880	the day of birth	1874:1889	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	7	62	theme	monoamine	1091:1099	arg1	levels					1101:1106	monoamine levels	1091:1106	monoamine levels	1091:1106	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	11	63	theme	bacterial	1518:1526	arg1	diversity					1528:1536	the bacterial diversity	1514:1536	the bacterial diversity of the intestinal microbiota	1514:1565	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	13	64	theme	developmental	1972:1984	arg1	period					1986:1991	the developmental period	1968:1991	the developmental period	1968:1991	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	14	65	from	behaviour	2191:2199	arg1	evidence					2140:2147	further evidence	2132:2147	further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life	2132:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	10	66	theme	lcFOS	1489:1493	arg1	mice					1499:1502	scGOS:lcFOS fed mice	1483:1502	scGOS:lcFOS fed mice	1483:1502	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	11	67	theme	intestinal	1545:1554	arg1	microbiota					1556:1565	the intestinal microbiota	1541:1565	the intestinal microbiota	1541:1565	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	7	68	theme	Tph2	1082:1085	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	68	theme	Tph2	1082:1085	arg1	time					992:995	time	992:995	time	992:995	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	68	theme	Tph2	1082:1085	arg1	expression					1051:1060	brain mRNA expression	1040:1060	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	68	theme	Tph2	1082:1085	arg1	levels					1101:1106	monoamine levels	1091:1106	monoamine levels	1091:1106	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	4	69	with	supplementation	594:608	arg1	mixture					629:635	this specific mixture	615:635	this specific mixture from the day of birth onwards	615:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	13	70	dep	Discussion	1817:1826	arg1	modulates					2016:2024	modulates	2016:2024	modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice	2016:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	13	70	dep	Discussion	1817:1826	arg1	associated					1894:1903	associated	1894:1903	is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life	1891:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	14	71	from	insights	2268:2275	arg1	interplay					2284:2292	the interplay	2280:2292	the interplay between the developing intestine and brain	2280:2335	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	4	72	theme	specific	620:627	arg1	mixture					629:635	this specific mixture	615:635	this specific mixture from the day of birth onwards	615:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	10	73	theme	scGOS	1483:1487	arg1	mice					1499:1502	scGOS:lcFOS fed mice	1483:1502	scGOS:lcFOS fed mice	1483:1502	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	11	74	theme	scGOS	1589:1593	arg1	diet					1601:1604	the scGOS:lcFOS diet	1585:1604	the scGOS:lcFOS diet	1585:1604	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	7	75	theme	Htr1b	1072:1076	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	75	theme	Htr1b	1072:1076	arg1	time					992:995	time	992:995	time	992:995	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	75	theme	Htr1b	1072:1076	arg1	expression					1051:1060	brain mRNA expression	1040:1060	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	75	theme	Htr1b	1072:1076	arg1	levels					1101:1106	monoamine levels	1091:1106	monoamine levels	1091:1106	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	8	76	theme	fed	1138:1140	arg1	mice					1142:1145	control fed mice	1130:1145	control fed mice	1130:1145	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	8	77	theme	anxiety-like	1184:1195	arg1	behaviour					1212:1220	reduced anxiety-like and repetitive behaviour	1176:1220	reduced anxiety-like and repetitive behaviour	1176:1220	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	13	78	from	behaviour	1951:1959	arg1	life					2006:2009	life	2006:2009	life	2006:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	7	79	theme	Htr1a	1065:1069	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	79	theme	Htr1a	1065:1069	arg1	time					992:995	time	992:995	time	992:995	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	79	theme	Htr1a	1065:1069	arg1	expression					1051:1060	brain mRNA expression	1040:1060	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	7	79	theme	Htr1a	1065:1069	arg1	levels					1101:1106	monoamine levels	1091:1106	monoamine levels	1091:1106	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	9	80	from	system	1293:1298	arg1	SSC					1357:1359	SSC	1357:1359	SSC	1357:1359	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	9	80	from	system	1293:1298	arg1	PFC					1326:1328	PFC	1326:1328	PFC	1326:1328	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	9	80	from	system	1293:1298	arg1	cortex					1318:1323	the prefrontal cortex	1303:1323	the prefrontal cortex (PFC)	1303:1329	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	9	80	from	system	1293:1298	arg1	cortex					1349:1354	somatosensory cortex	1335:1354	somatosensory cortex (SSC)	1335:1360	The serotonergic system in the prefrontal cortex (PFC) and somatosensory cortex (SSC) was affected by the scGOS:lcFOS.
30871432	4	81	theme	microbiota	695:704	arg1	development					706:716	intestinal microbiota development	684:716	intestinal microbiota development	684:716	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	4	82	theme	prebiotic	487:495	arg1	mixture					497:503	the prebiotic mixture	483:503	the prebiotic mixture of scGOS and lcFOS	483:522	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	4	83	theme	birth	653:657	arg1	onwards					659:665	birth onwards	653:665	birth onwards	653:665	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	7	84	theme	brain	1040:1044	arg1	expression					1051:1060	brain mRNA expression	1040:1060	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	11	85	dep	scGOS	1651:1655	arg1	mice					1683:1686	the control fed mice	1667:1686	the control fed mice	1667:1686	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	11	85	dep	scGOS	1651:1655	arg1	lcFOS					1657:1661	lcFOS	1657:1661	lcFOS	1657:1661	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	8	86	theme	repetitive	1201:1210	arg1	behaviour					1212:1220	reduced anxiety-like and repetitive behaviour	1176:1220	reduced anxiety-like and repetitive behaviour	1176:1220	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	1	87	theme	brain	220:224	arg1	development					226:236	brain development	220:236	brain development	220:236	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	8	88	theme	fed	1160:1162	arg1	mice					1164:1167	scGOS:lcFOS fed mice	1148:1167	scGOS:lcFOS fed mice	1148:1167	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	7	89	theme	fatty	1017:1021	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	4	90	theme	infant	539:544	arg1	formula					546:552	infant formula	539:552	infant formula	539:552	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	8	91	theme	social	1245:1250	arg1	behaviour					1252:1260	social behaviour	1245:1260	social behaviour	1245:1260	Results: Compared to control fed mice, scGOS:lcFOS fed mice showed reduced anxiety-like and repetitive behaviour over time and improved social behaviour in adulthood.
30871432	7	92	theme	caecal	998:1003	arg1	acids					1023:1027	caecal short-chain fatty acids	998:1027	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	0	93	from	behaviour	117:125	arg1	mice					142:145	male BALB/c mice	130:145	male BALB/c mice	130:145	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	12	94	located	observed	1789:1796	arg1	content					1808:1814	caecum content	1801:1814	caecum content	1801:1814	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	12	94	located	observed	1789:1796	arg2	saccharolytic					1722:1734	an increased saccharolytic	1709:1734	an increased saccharolytic	1709:1734	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	12	94	located	observed	1789:1796	arg2	activity					1775:1782	decreased proteolytic fermentation activity	1740:1782	decreased proteolytic fermentation activity	1740:1782	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	14	95	theme	developmental	2212:2224	arg1	stages					2226:2231	several developmental stages	2204:2231	several developmental stages throughout life	2204:2247	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	13	96	theme	intestinal	2062:2071	arg1	microbiota					2073:2082	the intestinal microbiota	2058:2082	the intestinal microbiota	2058:2082	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	5	97	theme	birth	785:789	arg1	day					778:780	day	778:780	day of birth	778:789	Method: Healthy male BALB/cByJ mice received, from day of birth, a dietary supplement with or without 3% scGOS:lcFOS (9:1).
30871432	3	98	from	benefit	444:450	arg1	behaviour					469:477	behaviour	469:477	behaviour	469:477	Several studies reported potential health benefit of prebiotics on behaviour.
30871432	1	99	theme	intestinal	163:172	arg1	essential					207:215	essential	207:215	essential	207:215	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	1	99	theme	intestinal	163:172	arg1	microbiota					174:183	The intestinal microbiota	159:183	The intestinal microbiota	159:183	Objective: The intestinal microbiota is acknowledged to be essential in brain development and behaviour.
30871432	0	100	theme	BALB/c	135:140	arg1	mice					142:145	male BALB/c mice	130:145	male BALB/c mice	130:145	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	11	101	theme	fed	1679:1681	arg1	mice					1683:1686	the control fed mice	1667:1686	the control fed mice	1667:1686	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	7	102	theme	faecal	956:961	arg1	compositions					974:985	faecal microbiota compositions	956:985	faecal microbiota compositions	956:985	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	0	103	from	onwards	70:76	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of dietary non-digestible oligosaccharides from birth onwards	0:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	0	103	from	onwards	70:76	arg1	oligosaccharides					42:57	dietary non-digestible oligosaccharides	19:57	dietary non-digestible oligosaccharides from birth onwards	19:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	0	104	theme	male	130:133	arg1	mice					142:145	male BALB/c mice	130:145	male BALB/c mice	130:145	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	13	105	theme	social	1944:1949	arg1	behaviour					1951:1959	reduced anxiety-like and improved social behaviour	1910:1959	reduced anxiety-like and improved social behaviour during the developmental period and later in life	1910:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	12	106	theme	caecum	1801:1806	arg1	content					1808:1814	caecum content	1801:1814	caecum content	1801:1814	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	3	107	theme	potential	427:435	arg1	benefit					444:450	potential health benefit	427:450	potential health benefit of prebiotics on behaviour	427:477	Several studies reported potential health benefit of prebiotics on behaviour.
30871432	14	108	theme	potential	2156:2164	arg1	impact					2166:2171	the potential impact	2152:2171	the potential impact of scGOS:lcFOS on behaviour	2152:2199	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	0	109	theme	non-digestible	27:40	arg1	oligosaccharides					42:57	dietary non-digestible oligosaccharides	19:57	dietary non-digestible oligosaccharides from birth onwards	19:76	Supplementation of dietary non-digestible oligosaccharides from birth onwards improve social and reduce anxiety-like behaviour in male BALB/c mice.
30871432	14	110	theme	developing	2306:2315	arg1	intestine					2317:2325	intestine	2317:2325	intestine	2317:2325	These data provide further evidence of the potential impact of scGOS:lcFOS on behaviour at several developmental stages throughout life and strengthen the insights in the interplay between the developing intestine and brain.
30871432	4	111	theme	supplementation	594:608	arg1	effects					575:581	the effects	571:581	the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice	571:724	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	3	112	theme	prebiotics	455:464	arg1	benefit					444:450	potential health benefit	427:450	potential health benefit of prebiotics on behaviour	427:477	Several studies reported potential health benefit of prebiotics on behaviour.
30871432	13	113	from	activity	2046:2053	arg1	mice					2107:2110	healthy male BALB/c mice	2087:2110	healthy male BALB/c mice	2087:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	13	114	theme	BALB/c	2100:2105	arg1	mice					2107:2110	healthy male BALB/c mice	2087:2110	healthy male BALB/c mice	2087:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	7	115	theme	mRNA	1046:1049	arg1	expression					1051:1060	brain mRNA expression	1040:1060	caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2	998:1085	We assessed faecal microbiota compositions over time, caecal short-chain fatty acids as well as brain mRNA expression of Htr1a, Htr1b and Tph2 and monoamine levels.
30871432	10	116	theme	factor	1453:1458	arg1	expression					1412:1421	mRNA expression	1407:1421	mRNA expression of brain-derived neurotrophic factor (Bdnf)	1407:1465	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	13	117	theme	anxiety-like	1918:1929	arg1	behaviour					1951:1959	reduced anxiety-like and improved social behaviour	1910:1959	reduced anxiety-like and improved social behaviour during the developmental period and later in life	1910:2009	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	2	118	theme	long-chain	359:368	arg1	lcFOS					394:398	lcFOS	394:398	lcFOS	394:398	Their composition can be modulated by prebiotics such as short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharide (lcFOS).
30871432	2	118	theme	long-chain	359:368	arg1	fructo-oligosaccharide					370:391	long-chain fructo-oligosaccharide	359:391	long-chain fructo-oligosaccharide (lcFOS)	359:399	Their composition can be modulated by prebiotics such as short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharide (lcFOS).
30871432	12	119	theme	proteolytic	1750:1760	arg1	activity					1775:1782	decreased proteolytic fermentation activity	1740:1782	decreased proteolytic fermentation activity	1740:1782	Moreover, an increased saccharolytic and decreased proteolytic fermentation activity were observed in caecum content.
30871432	10	120	theme	brain-derived	1426:1438	arg1	Bdnf					1461:1464	Bdnf	1461:1464	Bdnf	1461:1464	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	10	120	theme	brain-derived	1426:1438	arg1	factor					1453:1458	brain-derived neurotrophic factor	1426:1458	brain-derived neurotrophic factor (Bdnf)	1426:1465	In the PFC, mRNA expression of brain-derived neurotrophic factor (Bdnf) was enhanced in scGOS:lcFOS fed mice.
30871432	11	121	theme	microbiota	1607:1616	arg1	composition					1618:1628	microbiota composition	1607:1628	microbiota composition	1607:1628	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	4	122	from	effects	575:581	arg1	behaviour					670:678	behaviour	670:678	behaviour	670:678	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	4	122	from	effects	575:581	arg1	development					706:716	intestinal microbiota development	684:716	intestinal microbiota development	684:716	As the prebiotic mixture of scGOS and lcFOS is included in infant formula, we investigated the effects of dietary supplementation with this specific mixture from the day of birth onwards on behaviour and intestinal microbiota development in mice.
30871432	13	123	theme	microbiota	2073:2082	arg1	activity					2046:2053	activity	2046:2053	activity	2046:2053	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	13	123	theme	microbiota	2073:2082	arg1	composition					2030:2040	composition	2030:2040	composition	2030:2040	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30871432	11	124	theme	microbiota	1556:1565	arg1	diversity					1528:1536	the bacterial diversity	1514:1536	the bacterial diversity of the intestinal microbiota	1514:1565	Although the bacterial diversity of the intestinal microbiota was unaffected by the scGOS:lcFOS diet, microbiota composition differed between the scGOS:lcFOS and the control fed mice over time.
30871432	13	125	theme	healthy	2087:2093	arg1	mice					2107:2110	healthy male BALB/c mice	2087:2110	healthy male BALB/c mice	2087:2110	Discussion: Supplementing the diet with scGOS:lcFOS from the day of birth is associated with reduced anxiety-like and improved social behaviour during the developmental period and later in life, and modulates the composition and activity of the intestinal microbiota in healthy male BALB/c mice.
30620751	5	0	theme	antifungal	1003:1012	arg1	effects					1014:1020	antifungal effects	1003:1020	antifungal effects of CHOS fractions with varying average molecular weight	1003:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	6	1	from	accumulation	1311:1322	arg1	cytoplasm					1338:1346	the cytoplasm	1334:1346	the cytoplasm	1334:1346	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	5	2	dep	maximal	949:955	arg1	pH.					986:988	pH.	986:988	the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects	968:1210	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	4	3	dep	mL-1	780:783	arg1	μg					777:778	μg	777:778	μg	777:778	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	3	4	theme	C32	613:615	arg1	FA					600:601	FA	600:601	FA	600:601	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	4	theme	C32	613:615	arg1	acetylation					587:597	acetylation	587:597	acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	587:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	5	theme	soluble	467:473	arg1	CHOS					515:518	CHOS	515:518	CHOS	515:518	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	5	theme	soluble	467:473	arg1	chito-oligosaccharide					492:512	a soluble and well-defined chito-oligosaccharide	465:512	a soluble and well-defined chito-oligosaccharide (CHOS)	465:519	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	6	theme	antifungal	852:861	arg1	activity					863:870	The antifungal activity	848:870	The antifungal activity	848:870	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	6	theme	antifungal	852:861	arg1	fungicidal					890:899	fungicidal	890:899	fungicidal	890:899	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	6	theme	antifungal	852:861	arg1	proportional					919:930	proportional	919:930	proportional	919:930	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	4	7	theme	inhibitory	666:675	arg1	MIC					693:695	MIC	693:695	MIC	693:695	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	4	7	theme	inhibitory	666:675	arg1	concentrations					677:690	Minimal inhibitory concentrations	658:690	Minimal inhibitory concentrations (MIC)	658:696	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	3	8	from	acetylation	587:597	arg1	strains					649:655	52 medically relevant yeast strains	621:655	52 medically relevant yeast strains	621:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	9	theme	yeast	643:647	arg1	strains					649:655	52 medically relevant yeast strains	621:655	52 medically relevant yeast strains	621:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	10	theme	strongest	1183:1191	arg1	effects					1204:1210	the strongest inhibitory effects	1179:1210	the strongest inhibitory effects	1179:1210	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	7	11	theme	fungal	1401:1406	arg1	infections					1408:1417	fungal infections	1401:1417	fungal infections	1401:1417	Thus, C32 has potential to be used as a therapy for fungal infections.
30620751	5	12	from	pH	960:961	arg1	maximal					949:955	maximal	949:955	maximal	949:955	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	4	13	theme	Minimal	658:664	arg1	MIC					693:695	MIC	693:695	MIC	693:695	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	4	13	theme	Minimal	658:664	arg1	concentrations					677:690	Minimal inhibitory concentrations	658:690	Minimal inhibitory concentrations (MIC)	658:696	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	2	14	theme	human	273:277	arg1	medicine					279:286	human medicine	273:286	human medicine	273:286	However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans.
30620751	1	15	contain	have	169:172	arg2	potential					174:182	potential	174:182	potential	174:182	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	1	15	contain	have	169:172	arg1	chitosan					140:147	chitosan	140:147	chitosan	140:147	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	1	15	contain	have	169:172	arg1	derivatives					157:167	its derivatives	153:167	its derivatives	153:167	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	3	16	from	C32	613:615	arg1	strains					649:655	52 medically relevant yeast strains	621:655	52 medically relevant yeast strains	621:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	6	17	theme	cell	1291:1294	arg1	disruption					1296:1305	subsequent cell disruption	1280:1305	subsequent cell disruption	1280:1305	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	7	18	contain	has	1359:1361	arg2	potential					1363:1371	potential	1363:1371	potential	1363:1371	Thus, C32 has potential to be used as a therapy for fungal infections.
30620751	7	18	contain	has	1359:1361	arg1	C32					1355:1357	C32	1355:1357	C32	1355:1357	Thus, C32 has potential to be used as a therapy for fungal infections.
30620751	3	19	theme	relevant	634:641	arg1	strains					649:655	52 medically relevant yeast strains	621:655	52 medically relevant yeast strains	621:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	20	with	activity	453:460	arg1	DPn					560:562	DPn	560:562	DPn	560:562	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	20	with	activity	453:460	arg1	degree					552:557	an average polymerization degree	526:557	an average polymerization degree (DPn) of 32	526:569	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	20	with	activity	453:460	arg1	fraction					575:582	fraction	575:582	fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	575:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	21	dep	DP	1161:1162	arg1	i.e.					1156:1159	i.e.	1156:1159	i.e.	1156:1159	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	3	22	theme	average	529:535	arg1	DPn					560:562	DPn	560:562	DPn	560:562	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	22	theme	average	529:535	arg1	degree					552:557	an average polymerization degree	526:557	an average polymerization degree (DPn) of 32	526:569	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	0	23	theme	Antifungal	0:9	arg1	activity					11:18	Antifungal activity	0:18	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts	0:103	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts.
30620751	5	24	theme	average	1053:1059	arg1	weight					1071:1076	varying average molecular weight	1045:1076	varying average molecular weight	1045:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	3	25	theme	polymerization	537:550	arg1	DPn					560:562	DPn	560:562	DPn	560:562	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	25	theme	polymerization	537:550	arg1	degree					552:557	an average polymerization degree	526:557	an average polymerization degree (DPn) of 32	526:569	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	6	26	theme	Confocal	1213:1220	arg1	imaging					1222:1228	Confocal imaging	1213:1228	Confocal imaging	1213:1228	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	2	27	theme	compound	357:364	arg1	composition					338:348	the chemical composition	325:348	the chemical composition of the compound, which is not always the case for polymeric chitosans	325:418	However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans.
30620751	0	28	theme	well-defined	23:34	arg1	preparations					58:69	well-defined chito-oligosaccharide preparations	23:69	well-defined chito-oligosaccharide preparations	23:69	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts.
30620751	5	29	theme	molecular	1061:1069	arg1	weight					1071:1076	varying average molecular weight	1045:1076	varying average molecular weight	1045:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	30	with	fractions	1030:1038	arg1	weight					1071:1076	varying average molecular weight	1045:1076	varying average molecular weight	1045:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	1	31	theme	antifungal	119:128	arg1	activity					130:137	their antifungal activity	113:137	their antifungal activity	113:137	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	1	32	theme	yeast	208:212	arg1	infections					214:223	yeast infections	208:223	yeast infections in humans	208:233	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	3	33	from	strains	649:655	arg1	FA					600:601	FA	600:601	FA	600:601	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	33	from	strains	649:655	arg1	acetylation					587:597	acetylation	587:597	acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	587:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	34	theme	chito-oligosaccharide	492:512	arg1	activity					453:460	the antifungal activity	438:460	the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	438:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	6	35	theme	subsequent	1280:1289	arg1	disruption					1296:1305	subsequent cell disruption	1280:1305	subsequent cell disruption	1280:1305	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	2	36	theme	chemical	329:336	arg1	composition					338:348	the chemical composition	325:348	the chemical composition of the compound, which is not always the case for polymeric chitosans	325:418	However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans.
30620751	2	37	from	use	266:268	arg1	medicine					279:286	human medicine	273:286	human medicine	273:286	However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans.
30620751	0	38	theme	preparations	58:69	arg1	activity					11:18	Antifungal activity	0:18	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts	0:103	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts.
30620751	4	39	dep	μg	777:778	arg1	9.77					772:775	9.77	772:775	9.77	772:775	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	6	40	from	disruption	1296:1305	arg1	cytoplasm					1338:1346	the cytoplasm	1334:1346	the cytoplasm	1334:1346	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	5	41	theme	inhibitory	1193:1202	arg1	effects					1204:1210	the strongest inhibitory effects	1179:1210	the strongest inhibitory effects	1179:1210	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	0	42	theme	chito-oligosaccharide	36:56	arg1	preparations					58:69	well-defined chito-oligosaccharide preparations	23:69	well-defined chito-oligosaccharide preparations	23:69	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts.
30620751	5	43	theme	CHOS	1025:1028	arg1	fractions					1030:1038	CHOS fractions	1025:1038	CHOS fractions with varying average molecular weight	1025:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	4	44	dep	9.77	772:775	arg1	to					767:768	to	767:768	to	767:768	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	2	45	theme	polymeric	400:408	arg1	chitosans					410:418	polymeric chitosans	400:418	polymeric chitosans	400:418	However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans.
30620751	5	46	with	fractions	1099:1107	arg1	degree					1130:1135	an intermediate degree	1114:1135	an intermediate degree of polymerization	1114:1153	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	47	theme	varying	1045:1051	arg1	weight					1071:1076	varying average molecular weight	1045:1076	varying average molecular weight	1045:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	48	theme	fractions	1030:1038	arg1	effects					1014:1020	antifungal effects	1003:1020	antifungal effects of CHOS fractions with varying average molecular weight	1003:1076	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	3	49	theme	acetylation	587:597	arg1	DPn					560:562	DPn	560:562	DPn	560:562	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	49	theme	acetylation	587:597	arg1	degree					552:557	an average polymerization degree	526:557	an average polymerization degree (DPn) of 32	526:569	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	49	theme	acetylation	587:597	arg1	fraction					575:582	fraction	575:582	fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	575:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	50	contain	had	1175:1177	arg1	fractions					1099:1107	those fractions	1093:1107	those fractions	1093:1107	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	5	50	contain	had	1175:1177	arg2	effects					1204:1210	the strongest inhibitory effects	1179:1210	the strongest inhibitory effects	1179:1210	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	4	51	theme	yeast	718:722	arg1	species					724:730	yeast species	718:730	yeast species	718:730	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	4	52	theme	inhibition	790:799	arg1	patterns					801:808	inhibition patterns	790:808	inhibition patterns	790:808	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	1	53	from	infections	214:223	arg1	humans					228:233	humans	228:233	humans	228:233	Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans.
30620751	3	54	theme	antifungal	442:451	arg1	activity					453:460	the antifungal activity	438:460	the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	438:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	55	theme	intermediate	1117:1128	arg1	degree					1130:1135	an intermediate degree	1114:1135	an intermediate degree of polymerization	1114:1153	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	6	56	theme	C32	1327:1329	arg1	accumulation					1311:1322	accumulation	1311:1322	accumulation of C32 in the cytoplasm	1311:1346	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	6	56	theme	C32	1327:1329	arg1	disruption					1296:1305	subsequent cell disruption	1280:1305	subsequent cell disruption	1280:1305	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	6	57	theme	cell	1261:1264	arg1	surface					1266:1272	the cell surface	1257:1272	the cell surface	1257:1272	Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm.
30620751	4	58	dep	varied	698:703	arg1	mL-1					780:783	> 5000 to < 9.77 μg mL-1	760:783	> 5000 to < 9.77 μg mL-1	760:783	Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies.
30620751	5	59	dep	pH.	986:988	arg1	indicated					1078:1086	indicated	1078:1086	indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects	1078:1210	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30620751	0	60	theme	relevant	89:96	arg1	yeasts					98:103	medically relevant yeasts	79:103	medically relevant yeasts	79:103	Antifungal activity of well-defined chito-oligosaccharide preparations against medically relevant yeasts.
30620751	3	61	theme	32	568:569	arg1	DPn					560:562	DPn	560:562	DPn	560:562	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	61	theme	32	568:569	arg1	degree					552:557	an average polymerization degree	526:557	an average polymerization degree (DPn) of 32	526:569	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	61	theme	32	568:569	arg1	fraction					575:582	fraction	575:582	fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains	575:655	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	62	theme	well-defined	479:490	arg1	CHOS					515:518	CHOS	515:518	CHOS	515:518	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	3	62	theme	well-defined	479:490	arg1	chito-oligosaccharide					492:512	a soluble and well-defined chito-oligosaccharide	465:512	a soluble and well-defined chito-oligosaccharide (CHOS)	465:519	Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains.
30620751	5	63	theme	polymerization	1140:1153	arg1	degree					1130:1135	an intermediate degree	1114:1135	an intermediate degree of polymerization	1114:1153	The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects.
30689278	6	0	theme	cells	1433:1437	arg1	proliferation					1395:1407	proliferation	1395:1407	proliferation of rat bone-marrow stem cells	1395:1437	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	4	1	with	21 min	1108:1113	arg1	content					1138:1144	the increasing CS content	1120:1144	the increasing CS content	1120:1144	Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content.
30689278	1	2	theme	hydration	522:530	arg1	process					532:538	progressive hydration process	510:538	progressive hydration process of C3 S	510:546	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	3	theme	novel	147:151	arg1	bone					191:194	novel bioactive organic-inorganic composite bone	147:194	novel bioactive organic-inorganic composite bone	147:194	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	4	theme	degradability	609:621	arg1	properties					640:649	degradability and self-setting properties	609:649	degradability and self-setting properties	609:649	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	2	5	theme	single	878:883	arg1	component					885:893	each single component	873:893	each single component	873:893	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	6	6	theme	stem	1428:1431	arg1	cells					1433:1437	rat bone-marrow stem cells	1412:1437	rat bone-marrow stem cells	1412:1437	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	5	7	dep	composite	1151:1159	arg1	cements					1161:1167	cements	1161:1167	cements	1161:1167	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	8	8	dep	©	1617:1617	arg1	Inc.					1643:1646	Inc.	1643:1646	Inc.	1643:1646	© 2019 Wiley Periodicals, Inc.
30689278	1	9	theme	bioactive	153:161	arg1	bone					191:194	novel bioactive organic-inorganic composite bone	147:194	novel bioactive organic-inorganic composite bone	147:194	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	2	10	theme	higher	812:817	arg1	strength					831:838	extremely higher compressive strength	802:838	extremely higher compressive strength up to 92.41 MPa	802:854	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	1	11	theme	sodium	246:251	arg1	SA					263:264	SA	263:264	SA	263:264	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	11	theme	sodium	246:251	arg1	alginate					253:260	sodium alginate	246:260	sodium alginate (SA)	246:265	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	6	12	theme	bone-marrow	1416:1426	arg1	cells					1433:1437	rat bone-marrow stem cells	1412:1437	rat bone-marrow stem cells	1412:1437	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	7	13	theme	mechanical	1541:1550	arg1	performance					1552:1562	enhanced mechanical performance	1532:1562	enhanced mechanical performance	1532:1562	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	1	14	theme	internal	409:416	arg1	structure					418:426	internal structure	409:426	internal structure	409:426	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	15	theme	self-setting	627:638	arg1	properties					640:649	degradability and self-setting properties	609:649	degradability and self-setting properties	609:649	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	0	16	theme	in	89:90	arg1	bioactivity					98:108	in vitro bioactivity	89:108	in vitro bioactivity	89:108	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	7	17	theme	load-bearing	1591:1602	arg1	repair					1609:1614	load-bearing bone repair	1591:1614	load-bearing bone repair	1591:1614	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	3	18	theme	enhancement	1015:1025	arg1	phenomenon					1027:1036	this enhancement phenomenon	1010:1036	this enhancement phenomenon	1010:1036	The reinforcing mechanisms involving interfacial interaction and interior microstructure were proposed to explain this enhancement phenomenon.
30689278	4	19	theme	CS	1135:1136	arg1	content					1138:1144	the increasing CS content	1120:1144	the increasing CS content	1120:1144	Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content.
30689278	2	20	theme	compressive	819:829	arg1	strength					831:838	extremely higher compressive strength	802:838	extremely higher compressive strength up to 92.41 MPa	802:854	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	2	21	theme	C3	748:749	arg1	S/SA/CS					751:757	the C3 S/SA/CS composite cements	744:775	the C3 S/SA/CS composite cements (45/45/10 wt %)	744:791	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	2	21	theme	C3	748:749	arg1	%					790:790	45/45/10 wt %	778:790	45/45/10 wt %	778:790	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	1	22	theme	organic-inorganic	163:179	arg1	bone					191:194	novel bioactive organic-inorganic composite bone	147:194	novel bioactive organic-inorganic composite bone	147:194	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	9	23	dep	Res	1663:1665	arg1	2019					1706:1709	2019	1706:1709	2019	1706:1709	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.
30689278	9	23	dep	Res	1663:1665	arg1	B					1672:1672	Part B	1667:1672	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.	1648:1710	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.
30689278	9	23	dep	Res	1663:1665	arg1	Biomater					1680:1687	Biomater	1680:1687	Biomater	1680:1687	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.
30689278	7	24	theme	bioactive	1499:1507	arg1	composite					1509:1517	bioactive composite	1499:1517	bioactive composite	1499:1517	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	5	25	theme	body	1228:1231	arg1	solution					1239:1246	simulated body fluid solution	1218:1246	simulated body fluid solution	1218:1246	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	0	26	dep	in	89:90	arg1	vitro					92:96	vitro	92:96	vitro	92:96	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	1	27	theme	C3	543:544	arg1	S					546:546	C3 S	543:546	C3 S	543:546	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	28	theme	composite	181:189	arg1	bone					191:194	novel bioactive organic-inorganic composite bone	147:194	novel bioactive organic-inorganic composite bone	147:194	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	6	29	dep	cements	1335:1341	arg1	exhibited					1343:1351	exhibited	1343:1351	exhibited excellent cytocompatibility	1343:1379	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	6	29	dep	cements	1335:1341	arg1	increased					1385:1393	increased	1385:1393	increased proliferation of rat bone-marrow stem cells	1385:1437	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	1	30	theme	first	342:346	arg1	time					348:351	the first time	338:351	the first time	338:351	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	31	theme	S	546:546	arg1	process					532:538	progressive hydration process	510:538	progressive hydration process of C3 S	510:546	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	7	32	theme	enhanced	1532:1539	arg1	performance					1552:1562	enhanced mechanical performance	1532:1562	enhanced mechanical performance	1532:1562	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	5	33	theme	mineralization	1192:1205	arg1	ability					1207:1213	good apatite mineralization ability	1179:1213	good apatite mineralization ability	1179:1213	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	2	34	dep	S/SA/CS	751:757	arg1	cements					769:775	cements	769:775	cements	769:775	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	3	35	theme	interfacial	933:943	arg1	interaction					945:955	interfacial interaction	933:955	interfacial interaction	933:955	The reinforcing mechanisms involving interfacial interaction and interior microstructure were proposed to explain this enhancement phenomenon.
30689278	0	36	theme	organic-inorganic	16:32	arg1	bone					44:47	novel organic-inorganic composite bone	10:47	novel organic-inorganic composite bone	10:47	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	2	37	theme	hydration	720:728	arg1	combination					705:715	the synergistic combination	689:715	the synergistic combination of hydration and gelation	689:741	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	5	38	from	solution	1239:1246	arg1	buffer					1291:1296	phosphate buffer	1281:1296	phosphate buffer	1281:1296	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	1	39	theme	distinctive	553:563	arg1	capacity					574:581	distinctive gelation capacity	553:581	distinctive gelation capacity of SA	553:587	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	0	40	theme	novel	10:14	arg1	bone					44:47	novel organic-inorganic composite bone	10:47	novel organic-inorganic composite bone	10:47	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	7	41	theme	bone	1604:1607	arg1	repair					1609:1614	load-bearing bone repair	1591:1614	load-bearing bone repair	1591:1614	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	4	42	theme	increasing	1124:1133	arg1	CS					1135:1136	the increasing CS	1120:1136	the increasing CS content	1120:1144	Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content.
30689278	1	43	theme	gelation	565:572	arg1	capacity					574:581	distinctive gelation capacity	553:581	distinctive gelation capacity of SA	553:587	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	0	44	theme	bone	44:47	arg1	Design					0:5	Design	0:5	Design of novel organic-inorganic composite bone	0:47	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	3	45	theme	reinforcing	900:910	arg1	mechanisms					912:921	The reinforcing mechanisms	896:921	The reinforcing mechanisms involving interfacial interaction and interior microstructure	896:983	The reinforcing mechanisms involving interfacial interaction and interior microstructure were proposed to explain this enhancement phenomenon.
30689278	2	46	theme	composite	759:767	arg1	S/SA/CS					751:757	the C3 S/SA/CS composite cements	744:775	the C3 S/SA/CS composite cements (45/45/10 wt %)	744:791	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	2	46	theme	composite	759:767	arg1	%					790:790	45/45/10 wt %	778:790	45/45/10 wt %	778:790	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	1	47	theme	tricalcium	218:227	arg1	S					242:242	C3 S	239:242	C3 S	239:242	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	47	theme	tricalcium	218:227	arg1	silicate					229:236	tricalcium silicate	218:236	tricalcium silicate (C3 S)	218:243	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	48	theme	CS	672:673	arg1	addition					660:667	the addition	656:667	the addition of CS	656:673	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	49	theme	calcium	272:278	arg1	CS					301:302	CS	301:302	CS	301:302	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	49	theme	calcium	272:278	arg1	hemihydrate					288:298	calcium sulfate hemihydrate	272:298	calcium sulfate hemihydrate (CS)	272:303	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	0	50	theme	composite	34:42	arg1	bone					44:47	novel organic-inorganic composite bone	10:47	novel organic-inorganic composite bone	10:47	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	4	51	theme	setting	1063:1069	arg1	time					1071:1074	the final setting time	1053:1074	the final setting time	1053:1074	Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content.
30689278	5	52	theme	simulated	1218:1226	arg1	solution					1239:1246	simulated body fluid solution	1218:1246	simulated body fluid solution	1218:1246	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	5	53	theme	apatite	1184:1190	arg1	ability					1207:1213	good apatite mineralization ability	1179:1213	good apatite mineralization ability	1179:1213	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	1	54	theme	sulfate	280:286	arg1	CS					301:302	CS	301:302	CS	301:302	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	54	theme	sulfate	280:286	arg1	hemihydrate					288:298	calcium sulfate hemihydrate	272:298	calcium sulfate hemihydrate (CS)	272:303	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	5	55	from	rate	1273:1276	arg1	buffer					1291:1296	phosphate buffer	1281:1296	phosphate buffer	1281:1296	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	1	56	theme	special	359:365	arg1	method					367:372	a special method	357:372	a special method designing material composition and internal structure	357:426	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	2	57	theme	synergistic	693:703	arg1	combination					705:715	the synergistic combination	689:715	the synergistic combination of hydration and gelation	689:741	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	6	58	theme	excellent	1353:1361	arg1	cytocompatibility					1363:1379	excellent cytocompatibility	1353:1379	excellent cytocompatibility	1353:1379	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	5	59	theme	good	1179:1182	arg1	ability					1207:1213	good apatite mineralization ability	1179:1213	good apatite mineralization ability	1179:1213	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	5	60	dep	solution	1239:1246	arg1	solution					1298:1305	solution	1298:1305	solution	1298:1305	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	9	61	theme	Part	1667:1670	arg1	B					1672:1672	Part B	1667:1672	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.	1648:1710	J Biomed Mater Res Part B: Appl Biomater 107B: 2365-2377, 2019.
30689278	1	62	theme	mechanical	474:483	arg1	performance					485:495	mechanical performance	474:495	mechanical performance	474:495	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	5	63	theme	degradation	1261:1271	arg1	rate					1273:1276	moderate degradation rate	1252:1276	moderate degradation rate in phosphate buffer	1252:1296	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	5	64	contain	possessed	1169:1177	arg2	ability					1207:1213	good apatite mineralization ability	1179:1213	good apatite mineralization ability	1179:1213	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	5	64	contain	possessed	1169:1177	arg1	composite					1151:1159	composite	1151:1159	composite	1151:1159	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	3	65	theme	interior	961:968	arg1	microstructure					970:983	interior microstructure	961:983	interior microstructure	961:983	The reinforcing mechanisms involving interfacial interaction and interior microstructure were proposed to explain this enhancement phenomenon.
30689278	7	66	dep	preparation	1484:1494	arg1	cements					1519:1525	cements	1519:1525	cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair	1519:1614	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	1	67	theme	SA	586:587	arg1	capacity					574:581	distinctive gelation capacity	553:581	distinctive gelation capacity of SA	553:587	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	5	68	theme	moderate	1252:1259	arg1	rate					1273:1276	moderate degradation rate	1252:1276	moderate degradation rate in phosphate buffer	1252:1296	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	1	69	theme	material	384:391	arg1	composition					393:403	material composition	384:403	material composition	384:403	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	2	70	theme	gelation	734:741	arg1	combination					705:715	the synergistic combination	689:715	the synergistic combination of hydration and gelation	689:741	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	6	71	theme	rat	1412:1414	arg1	cells					1433:1437	rat bone-marrow stem cells	1412:1437	rat bone-marrow stem cells	1412:1437	What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone-marrow stem cells.
30689278	1	72	theme	C3	239:240	arg1	S					242:242	C3 S	239:242	C3 S	239:242	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	1	72	theme	C3	239:240	arg1	silicate					229:236	tricalcium silicate	218:236	tricalcium silicate (C3 S)	218:243	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	0	73	theme	compressive	67:77	arg1	strength					79:86	high compressive strength	62:86	high compressive strength	62:86	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	2	74	theme	wt	787:788	arg1	S/SA/CS					751:757	the C3 S/SA/CS composite cements	744:775	the C3 S/SA/CS composite cements (45/45/10 wt %)	744:791	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	2	74	theme	wt	787:788	arg1	%					790:790	45/45/10 wt %	778:790	45/45/10 wt %	778:790	Depending on the synergistic combination of hydration and gelation, the C3 S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component.
30689278	0	75	theme	high	62:65	arg1	strength					79:86	high compressive strength	62:86	high compressive strength	62:86	Design of novel organic-inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility.
30689278	4	76	theme	final	1057:1061	arg1	time					1071:1074	the final setting time	1053:1074	the final setting time	1053:1074	Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content.
30689278	1	77	theme	progressive	510:520	arg1	process					532:538	progressive hydration process	510:538	progressive hydration process of C3 S	510:546	In this work, novel bioactive organic-inorganic composite bone cements consisting of tricalcium silicate (C3 S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3 S with distinctive gelation capacity of SA and further improve degradability and self-setting properties with the addition of CS.
30689278	7	78	theme	composite	1509:1517	arg1	preparation					1484:1494	the preparation	1480:1494	the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair	1480:1614	This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load-bearing bone repair.
30689278	5	79	theme	phosphate	1281:1289	arg1	buffer					1291:1296	phosphate buffer	1281:1296	phosphate buffer	1281:1296	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
30689278	5	80	theme	fluid	1233:1237	arg1	solution					1239:1246	simulated body fluid solution	1218:1246	simulated body fluid solution	1218:1246	The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution.
29525155	5	0	theme	size	759:762	arg1	distribution					764:775	granule size distribution	751:775	granule size distribution	751:775	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	4	1	theme	Maize	499:503	arg1	starches					516:523	Maize and potato starches	499:523	Maize and potato starches	499:523	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	1	2	theme	sweetpotato	137:147	arg1	diversity					149:157	sweetpotato diversity	137:157	sweetpotato diversity	137:157	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	5	3	from	composition	701:711	arg1	degree					670:675	a degree	668:675	a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches	668:837	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	7	4	theme	internal	973:980	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	2	5	theme	granular	337:344	arg1	structure					346:354	the molecular and granular structure	319:354	the molecular and granular structure of the starches	319:370	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	5	6	from	unit	714:717	arg1	degree					670:675	a degree	668:675	a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches	668:837	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	4	7	theme	potato	509:514	arg1	starches					516:523	Maize and potato starches	499:523	Maize and potato starches	499:523	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	7	8	theme	unit	982:985	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	1	9	theme	Zealand	49:55	arg1	kumara					72:77	kumara	72:77	kumara	72:77	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	1	9	theme	Zealand	49:55	arg1	sweetpotatoes					57:69	New Zealand sweetpotatoes	45:69	New Zealand sweetpotatoes (kumara) (Ipomoea batatas)	45:96	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	1	9	theme	Zealand	49:55	arg1	batatas					89:95	Ipomoea batatas	81:95	Ipomoea batatas	81:95	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	4	10	theme	commercial	590:599	arg1	sources					608:614	the most important commercial starch sources	571:614	the most important commercial starch sources	571:614	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	5	11	theme	internal	723:730	arg1	composition					738:748	internal chain composition	723:748	internal chain composition	723:748	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	2	12	theme	structure	346:354	arg1	characterization					299:314	the characterization	295:314	the characterization of the molecular and granular structure of the starches	295:370	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	3	13	theme	enzymatic	477:485	arg1	techniques					487:496	chromatographic and enzymatic techniques	457:496	chromatographic and enzymatic techniques	457:496	In particular, the internal molecular structure of the amylopectins was detailed by chromatographic and enzymatic techniques.
29525155	5	14	theme	chain	732:736	arg1	composition					738:748	internal chain composition	723:748	internal chain composition	723:748	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	7	15	theme	long	968:971	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	2	16	theme	commercial	252:261	arg1	significance					263:274	commercial significance	252:274	commercial significance	252:274	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	4	17	theme	important	580:588	arg1	sources					608:614	the most important commercial starch sources	571:614	the most important commercial starch sources	571:614	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	3	18	theme	amylopectins	428:439	arg1	structure					411:419	the internal molecular structure	388:419	the internal molecular structure of the amylopectins	388:439	In particular, the internal molecular structure of the amylopectins was detailed by chromatographic and enzymatic techniques.
29525155	5	19	theme	crystallinity	792:804	arg1	degree					782:787	degree	782:787	degree	782:787	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	19	theme	crystallinity	792:804	arg1	composition					701:711	amylose composition	693:711	amylose composition	693:711	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	19	theme	crystallinity	792:804	arg1	distribution					764:775	granule size distribution	751:775	granule size distribution	751:775	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	19	theme	crystallinity	792:804	arg1	composition					738:748	internal chain composition	723:748	internal chain composition	723:748	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	19	theme	crystallinity	792:804	arg1	unit					714:717	unit	714:717	unit	714:717	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	6	20	theme	CA-type	876:882	arg1	polymorph					884:892	CA-type polymorph	876:892	CA-type polymorph	876:892	All the sweetpotato starches showed CA-type polymorph.
29525155	8	21	theme	starches	1093:1100	arg1	structure					1068:1076	the structure	1064:1076	the structure of sweetpotato starches	1064:1100	The differences in the structure of sweetpotato starches suggest differences in physicochemical properties.
29525155	7	22	theme	short	958:962	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	7	23	contain	have	924:927	arg1	amylopectins					911:922	The sweetpotato amylopectins	895:922	The sweetpotato amylopectins	895:922	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	7	23	contain	have	924:927	arg2	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	7	23	contain	have	924:927	arg2	amounts					942:948	intermediate amounts	929:948	intermediate amounts of both short and long internal unit chains	929:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	2	24	theme	starches	363:370	arg1	structure					346:354	the molecular and granular structure	319:354	the molecular and granular structure of the starches	319:370	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	0	25	theme	Zealand	17:23	arg1	Structure					0:8	Structure	0:8	Structure of New Zealand	0:23	Structure of New Zealand sweetpotato starch.
29525155	5	26	theme	amylose	693:699	arg1	composition					701:711	amylose composition	693:711	amylose composition	693:711	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	1	27	theme	Ipomoea	81:87	arg1	sweetpotatoes					57:69	New Zealand sweetpotatoes	45:69	New Zealand sweetpotatoes (kumara) (Ipomoea batatas)	45:96	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	1	27	theme	Ipomoea	81:87	arg1	batatas					89:95	Ipomoea batatas	81:95	Ipomoea batatas	81:95	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	5	28	theme	sweetpotato	818:828	arg1	starches					830:837	the 7 sweetpotato starches	812:837	the 7 sweetpotato starches	812:837	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	0	29	theme	New	13:15	arg1	Zealand					17:23	New Zealand	13:23	New Zealand	13:23	Structure of New Zealand sweetpotato starch.
29525155	8	30	from	differences	1049:1059	arg1	structure					1068:1076	the structure	1064:1076	the structure of sweetpotato starches	1064:1100	The differences in the structure of sweetpotato starches suggest differences in physicochemical properties.
29525155	3	31	theme	chromatographic	457:471	arg1	techniques					487:496	chromatographic and enzymatic techniques	457:496	chromatographic and enzymatic techniques	457:496	In particular, the internal molecular structure of the amylopectins was detailed by chromatographic and enzymatic techniques.
29525155	3	32	theme	internal	392:399	arg1	structure					411:419	the internal molecular structure	388:419	the internal molecular structure of the amylopectins	388:439	In particular, the internal molecular structure of the amylopectins was detailed by chromatographic and enzymatic techniques.
29525155	8	33	theme	physicochemical	1125:1139	arg1	properties					1141:1150	physicochemical properties	1125:1150	physicochemical properties	1125:1150	The differences in the structure of sweetpotato starches suggest differences in physicochemical properties.
29525155	5	34	from	composition	738:748	arg1	degree					670:675	a degree	668:675	a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches	668:837	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	3	35	theme	molecular	401:409	arg1	structure					411:419	the internal molecular structure	388:419	the internal molecular structure of the amylopectins	388:439	In particular, the internal molecular structure of the amylopectins was detailed by chromatographic and enzymatic techniques.
29525155	4	36	theme	normal	530:535	arg1	contents					545:552	normal amylose contents	530:552	normal amylose contents	530:552	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	6	37	theme	sweetpotato	848:858	arg1	starches					860:867	All the sweetpotato starches	840:867	All the sweetpotato starches	840:867	All the sweetpotato starches showed CA-type polymorph.
29525155	8	38	from	differences	1110:1120	arg1	properties					1141:1150	physicochemical properties	1125:1150	physicochemical properties	1125:1150	The differences in the structure of sweetpotato starches suggest differences in physicochemical properties.
29525155	7	39	theme	sources	1036:1042	arg1	amylopectins					1000:1011	amylopectins	1000:1011	amylopectins of different botanical sources	1000:1042	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	4	40	theme	amylose	537:543	arg1	contents					545:552	normal amylose contents	530:552	normal amylose contents	530:552	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	4	41	with	starches	516:523	arg1	contents					545:552	normal amylose contents	530:552	normal amylose contents	530:552	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	2	42	with	varieties	237:245	arg1	significance					263:274	commercial significance	252:274	commercial significance	252:274	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	7	43	theme	different	1016:1024	arg1	sources					1036:1042	different botanical sources	1016:1042	different botanical sources	1016:1042	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	2	44	theme	molecular	323:331	arg1	structure					346:354	the molecular and granular structure	319:354	the molecular and granular structure of the starches	319:370	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	1	45	theme	New	45:47	arg1	kumara					72:77	kumara	72:77	kumara	72:77	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	1	45	theme	New	45:47	arg1	sweetpotatoes					57:69	New Zealand sweetpotatoes	45:69	New Zealand sweetpotatoes (kumara) (Ipomoea batatas)	45:96	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	1	45	theme	New	45:47	arg1	batatas					89:95	Ipomoea batatas	81:95	Ipomoea batatas	81:95	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	5	46	theme	diversity	680:688	arg1	degree					670:675	a degree	668:675	a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches	668:837	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	7	47	theme	botanical	1026:1034	arg1	sources					1036:1042	different botanical sources	1016:1042	different botanical sources	1016:1042	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	1	48	theme	unique	108:113	arg1	resources					123:131	unique genetic resources	108:131	unique genetic resources for sweetpotato diversity	108:157	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	7	49	theme	intermediate	929:940	arg1	amounts					942:948	intermediate amounts	929:948	intermediate amounts of both short and long internal unit chains	929:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	7	49	theme	intermediate	929:940	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	5	50	from	diversity	680:688	arg1	composition					701:711	amylose composition	693:711	amylose composition	693:711	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	50	from	diversity	680:688	arg1	composition					738:748	internal chain composition	723:748	internal chain composition	723:748	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	50	from	diversity	680:688	arg1	unit					714:717	unit	714:717	unit	714:717	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	2	51	theme	sweetpotato	225:235	arg1	varieties					237:245	7 New Zealand sweetpotato varieties	211:245	7 New Zealand sweetpotato varieties with commercial significance	211:274	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	1	52	theme	genetic	115:121	arg1	resources					123:131	unique genetic resources	108:131	unique genetic resources for sweetpotato diversity	108:157	New Zealand sweetpotatoes (kumara) (Ipomoea batatas) represent unique genetic resources for sweetpotato diversity, though they are much under-studied.
29525155	7	53	theme	sweetpotato	899:909	arg1	amylopectins					911:922	The sweetpotato amylopectins	895:922	The sweetpotato amylopectins	895:922	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	4	54	theme	starch	601:606	arg1	sources					608:614	the most important commercial starch sources	571:614	the most important commercial starch sources	571:614	Maize and potato starches with normal amylose contents, which are among the most important commercial starch sources, were employed for comparison.
29525155	8	55	theme	sweetpotato	1081:1091	arg1	starches					1093:1100	sweetpotato starches	1081:1100	sweetpotato starches	1081:1100	The differences in the structure of sweetpotato starches suggest differences in physicochemical properties.
29525155	2	56	theme	Zealand	217:223	arg1	varieties					237:245	7 New Zealand sweetpotato varieties	211:245	7 New Zealand sweetpotato varieties with commercial significance	211:274	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	5	57	from	degree	670:675	arg1	composition					701:711	amylose composition	693:711	amylose composition	693:711	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	57	from	degree	670:675	arg1	composition					738:748	internal chain composition	723:748	internal chain composition	723:748	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	57	from	degree	670:675	arg1	unit					714:717	unit	714:717	unit	714:717	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	5	58	theme	granule	751:757	arg1	distribution					764:775	granule size distribution	751:775	granule size distribution	751:775	The results revealed a degree of diversity in amylose composition, unit and internal chain composition, granule size distribution, and degree of crystallinity among the 7 sweetpotato starches.
29525155	2	59	theme	New	213:215	arg1	varieties					237:245	7 New Zealand sweetpotato varieties	211:245	7 New Zealand sweetpotato varieties with commercial significance	211:274	In this study, 7 New Zealand sweetpotato varieties with commercial significance were collected for the characterization of the molecular and granular structure of the starches.
29525155	7	60	theme	chains	987:992	arg1	amounts					942:948	intermediate amounts	929:948	intermediate amounts of both short and long internal unit chains	929:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
29525155	7	60	theme	chains	987:992	arg1	chains					987:992	both short and long internal unit chains	953:992	both short and long internal unit chains	953:992	The sweetpotato amylopectins have intermediate amounts of both short and long internal unit chains among amylopectins of different botanical sources.
31826510	4	0	theme	sugar	716:720	arg1	residues					722:729	sugar residues	716:729	sugar residues	716:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	7	1	theme	food	1174:1177	arg1	industries					1191:1200	food or cosmetic industries	1174:1200	food or cosmetic industries	1174:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	2	2	theme	morphology	400:409	arg1	terms					391:395	terms	391:395	terms of morphology, chemical composition, and rheological and emulsifying properties	391:475	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	5	3	theme	entangled	794:802	arg1	structure					804:812	entangled structure	794:812	entangled structure	794:812	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	1	4	contain	have	147:150	arg1	polysaccharides					105:119	The extracellular polysaccharides	87:119	The extracellular polysaccharides produced by cyanobacteria	87:145	The extracellular polysaccharides produced by cyanobacteria have distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine.
31826510	1	4	contain	have	147:150	arg2	characteristics					164:178	distinctive characteristics	152:178	distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine	152:263	The extracellular polysaccharides produced by cyanobacteria have distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine.
31826510	3	5	theme	residues	543:550	arg1	%					529:529	11 %	526:529	11 % of sulfated residues	526:550	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	3	5	theme	residues	543:550	arg1	residues					543:550	sulfated residues	534:550	sulfated residues	534:550	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	5	6	theme	mass	761:764	arg1	fractions					766:774	high molecular mass fractions	746:774	high molecular mass fractions (above 1 MDa)	746:788	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	6	theme	mass	761:764	arg1	1 MDa					783:787	above 1 MDa	777:787	above 1 MDa	777:787	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	2	7	theme	cyanobacterial	328:341	arg1	strain					343:348	a marine cyanobacterial strain	319:348	a marine cyanobacterial strain	319:348	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	4	8	theme	glycosidic-substitution	606:628	arg1	analysis					630:637	The glycosidic-substitution analysis	602:637	The glycosidic-substitution analysis	602:637	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	2	9	theme	marine	321:326	arg1	strain					343:348	a marine cyanobacterial strain	319:348	a marine cyanobacterial strain	319:348	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	5	10	theme	high	886:889	arg1	activity					903:910	high emulsifying activity	886:910	high emulsifying activity	886:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	11	from	viscosity	851:859	arg1	solutions					872:880	aqueous solutions	864:880	aqueous solutions	864:880	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	11	from	viscosity	851:859	arg1	activity					903:910	high emulsifying activity	886:910	high emulsifying activity	886:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	12	theme	aqueous	864:870	arg1	solutions					872:880	aqueous solutions	864:880	aqueous solutions	864:880	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	4	13	theme	branched	657:664	arg1	structure					683:691	a highly branched complex chemical structure	648:691	a highly branched complex chemical structure with a large number of sugar residues	648:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	2	14	theme	rheological	438:448	arg1	properties					466:475	rheological and emulsifying properties	438:475	properties	466:475	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	5	15	theme	above	777:781	arg1	fractions					766:774	high molecular mass fractions	746:774	high molecular mass fractions (above 1 MDa)	746:788	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	15	theme	above	777:781	arg1	1 MDa					783:787	above 1 MDa	777:787	above 1 MDa	777:787	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	7	16	theme	emulsifying/thickening	1142:1163	arg1	agent					1165:1169	emulsifying/thickening agent	1142:1169	emulsifying/thickening agent in food or cosmetic industries	1142:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	7	17	theme	cosmetic	1182:1189	arg1	industries					1191:1200	food or cosmetic industries	1174:1200	food or cosmetic industries	1174:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	4	18	with	structure	683:691	arg1	number					706:711	a large number	698:711	a large number of sugar residues	698:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	3	19	theme	71	494:495	arg1	%					497:497	%	497:497	%	497:497	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	5	20	dep	cyanoflan	736:744	arg1	fractions					766:774	high molecular mass fractions	746:774	high molecular mass fractions (above 1 MDa)	746:788	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	20	dep	cyanoflan	736:744	arg1	1 MDa					783:787	above 1 MDa	777:787	above 1 MDa	777:787	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	1	21	theme	extracellular	91:103	arg1	polysaccharides					105:119	The extracellular polysaccharides	87:119	The extracellular polysaccharides produced by cyanobacteria	87:145	The extracellular polysaccharides produced by cyanobacteria have distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine.
31826510	5	22	theme	high	837:840	arg1	viscosity					851:859	its high apparent viscosity	833:859	its high apparent viscosity in aqueous solutions and high emulsifying activity	833:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	2	23	theme	named	354:358	arg1	cyanoflan					360:368	named cyanoflan	354:368	named cyanoflan	354:368	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	2	24	theme	composition	421:431	arg1	terms					391:395	terms	391:395	terms of morphology, chemical composition, and rheological and emulsifying properties	391:475	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	0	25	theme	cyanobacterial	13:26	arg1	polymer					50:56	A cyanobacterial sulfated carbohydrate polymer	11:56	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.	0:85	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
31826510	7	26	theme	biotechnological	1103:1118	arg1	applications					1120:1131	different biotechnological applications	1093:1131	different biotechnological applications	1093:1131	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	4	27	theme	complex	666:672	arg1	structure					683:691	a highly branched complex chemical structure	648:691	a highly branched complex chemical structure with a large number of sugar residues	648:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	3	28	theme	dry	590:592	arg1	weight					594:599	dry weight	590:599	dry weight	590:599	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	4	29	theme	residues	722:729	arg1	number					706:711	a large number	698:711	a large number of sugar residues	698:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	6	30	theme	non-Newtonian	939:951	arg1	It					913:914	It	913:914	It	913:914	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	6	30	theme	non-Newtonian	939:951	arg1	fluid					953:957	a typical non-Newtonian fluid	929:957	a typical non-Newtonian fluid with pseudoplastic behavior	929:985	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	2	31	theme	properties	466:475	arg1	terms					391:395	terms	391:395	terms of morphology, chemical composition, and rheological and emulsifying properties	391:475	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	0	32	theme	carbohydrate	37:48	arg1	polymer					50:56	A cyanobacterial sulfated carbohydrate polymer	11:56	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.	0:85	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
31826510	5	33	theme	high	746:749	arg1	mass					761:764	high molecular mass	746:764	high molecular mass fractions (above 1 MDa)	746:788	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	3	34	theme	%	497:497	arg1	content					512:518	a 71 % carbohydrate content	492:518	a 71 % carbohydrate content	492:518	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	4	35	theme	large	700:704	arg1	number					706:711	a large number	698:711	a large number of sugar residues	698:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	2	36	theme	emulsifying	454:464	arg1	properties					466:475	rheological and emulsifying properties	438:475	properties	466:475	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	0	37	theme	sulfated	28:35	arg1	polymer					50:56	A cyanobacterial sulfated carbohydrate polymer	11:56	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.	0:85	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
31826510	7	38	from	agent	1165:1169	arg1	industries					1191:1200	food or cosmetic industries	1174:1200	food or cosmetic industries	1174:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	5	39	theme	molecular	751:759	arg1	mass					761:764	high molecular mass	746:764	high molecular mass fractions (above 1 MDa)	746:788	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	5	40	theme	apparent	842:849	arg1	viscosity					851:859	its high apparent viscosity	833:859	its high apparent viscosity in aqueous solutions and high emulsifying activity	833:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	7	41	theme	carbohydrate	1052:1063	arg1	cyanoflan					1027:1035	cyanoflan	1027:1035	cyanoflan	1027:1035	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	7	41	theme	carbohydrate	1052:1063	arg1	polymer					1065:1071	a versatile carbohydrate polymer	1040:1071	a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries	1040:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	3	42	theme	carbohydrate	499:510	arg1	content					512:518	a 71 % carbohydrate content	492:518	a 71 % carbohydrate content	492:518	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	7	43	used	used	1085:1088	arg2	polymer					1065:1071	a versatile carbohydrate polymer	1040:1071	a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries	1040:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	7	43	used	used	1085:1088	arg2	cyanoflan					1027:1035	cyanoflan	1027:1035	cyanoflan	1027:1035	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	3	44	theme	sulfated	534:541	arg1	residues					543:550	sulfated residues	534:550	sulfated residues	534:550	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	3	45	theme	weight	594:599	arg1	%					585:585	4 %	583:585	4 % of dry weight	583:599	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	3	45	theme	weight	594:599	arg1	weight					594:599	dry weight	590:599	dry weight	590:599	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	7	46	theme	different	1093:1101	arg1	applications					1120:1131	different biotechnological applications	1093:1131	different biotechnological applications	1093:1131	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	6	47	theme	pseudoplastic	964:976	arg1	behavior					978:985	pseudoplastic behavior	964:985	pseudoplastic behavior	964:985	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	3	48	contain	has	488:490	arg1	Cyanoflan					478:486	Cyanoflan	478:486	Cyanoflan	478:486	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	3	48	contain	has	488:490	arg2	content					512:518	a 71 % carbohydrate content	492:518	a 71 % carbohydrate content	492:518	Cyanoflan has a 71 % carbohydrate content, with 11 % of sulfated residues, while the protein account for 4 % of dry weight.
31826510	7	49	theme	versatile	1042:1050	arg1	cyanoflan					1027:1035	cyanoflan	1027:1035	cyanoflan	1027:1035	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	7	49	theme	versatile	1042:1050	arg1	polymer					1065:1071	a versatile carbohydrate polymer	1040:1071	a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries	1040:1200	Altogether, these results confirm that cyanoflan is a versatile carbohydrate polymer that can be used in different biotechnological applications, such as emulsifying/thickening agent in food or cosmetic industries.
31826510	1	50	theme	distinctive	152:162	arg1	characteristics					164:178	distinctive characteristics	152:178	distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine	152:263	The extracellular polysaccharides produced by cyanobacteria have distinctive characteristics that make them promising for applications ranging from bioremediation to biomedicine.
31826510	6	51	theme	typical	931:937	arg1	It					913:914	It	913:914	It	913:914	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	6	51	theme	typical	931:937	arg1	fluid					953:957	a typical non-Newtonian fluid	929:957	a typical non-Newtonian fluid with pseudoplastic behavior	929:985	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	5	52	with	consistent	817:826	arg1	viscosity					851:859	its high apparent viscosity	833:859	its high apparent viscosity in aqueous solutions and high emulsifying activity	833:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	6	53	with	fluid	953:957	arg1	behavior					978:985	pseudoplastic behavior	964:985	pseudoplastic behavior	964:985	It showed to be a typical non-Newtonian fluid with pseudoplastic behavior.
31826510	4	54	theme	chemical	674:681	arg1	structure					683:691	a highly branched complex chemical structure	648:691	a highly branched complex chemical structure with a large number of sugar residues	648:729	The glycosidic-substitution analysis revealed a highly branched complex chemical structure with a large number of sugar residues.
31826510	2	55	theme	sulfated	283:290	arg1	polysaccharide					292:305	a sulfated polysaccharide	281:305	a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan	281:368	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	0	56	with	polymer	50:56	arg1	properties					75:84	emulsifying properties	63:84	emulsifying properties	63:84	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
31826510	2	57	theme	chemical	412:419	arg1	composition					421:431	chemical composition	412:431	chemical composition	412:431	In this study, a sulfated polysaccharide produced by a marine cyanobacterial strain and named cyanoflan was characterized in terms of morphology, chemical composition, and rheological and emulsifying properties.
31826510	5	58	theme	emulsifying	891:901	arg1	activity					903:910	high emulsifying activity	886:910	high emulsifying activity	886:910	The cyanoflan high molecular mass fractions (above 1 MDa) and entangled structure is consistent with its high apparent viscosity in aqueous solutions and high emulsifying activity.
31826510	0	59	theme	emulsifying	63:73	arg1	properties					75:84	emulsifying properties	63:84	emulsifying properties	63:84	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
31826510	0	60	dep	Cyanoflan	0:8	arg1	polymer					50:56	A cyanobacterial sulfated carbohydrate polymer	11:56	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.	0:85	Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties.
30978420	5	0	theme	molecular	602:610	arg1	weight					612:617	the molecular weight	598:617	the molecular weight of active polymer	598:635	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	2	1	theme	modified	368:375	arg1	MCCs					377:380	modified MCCs	368:380	modified MCCs	368:380	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	10	2	theme	other	1282:1286	arg1	sectors					1300:1306	other food allied sectors	1282:1306	other food allied sectors	1282:1306	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	10	3	theme	composites	1245:1254	arg1	properties					1222:1231	the original properties	1209:1231	the original properties of un-dried composites	1209:1254	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	10	4	theme	un-dried	1236:1243	arg1	composites					1245:1254	un-dried composites	1236:1254	un-dried composites	1236:1254	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	5	5	theme	active	622:627	arg1	polymer					629:635	active polymer	622:635	active polymer	622:635	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	0	6	from	characterization	68:83	arg1	Stabilization					109:121	the Stabilization	105:121	the Stabilization of Pickering emulsions	105:144	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	5	7	theme	physicochemical	686:700	arg1	properties					702:711	the physicochemical properties	682:711	the physicochemical properties	682:711	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	5	8	theme	polymer	629:635	arg1	weight					612:617	the molecular weight	598:617	the molecular weight of active polymer	598:635	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	0	9	theme	emulsions	136:144	arg1	Stabilization					109:121	the Stabilization	105:121	the Stabilization of Pickering emulsions	105:144	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	6	10	with	CMCNa	765:769	arg1	weight					791:796	high molecular weight	776:796	high molecular weight	776:796	The composites prepared from CMCNa with high molecular weight were more stable than others.
30978420	5	11	contain	had	637:639	arg1	weight					612:617	the molecular weight	598:617	the molecular weight of active polymer	598:635	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	5	11	contain	had	637:639	arg2	influence					669:677	an amenable and significant influence	641:677	an amenable and significant influence	641:677	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	0	12	theme	Pickering	126:134	arg1	emulsions					136:144	Pickering emulsions	126:144	Pickering emulsions	126:144	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	5	13	theme	amenable	644:651	arg1	influence					669:677	an amenable and significant influence	641:677	an amenable and significant influence	641:677	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	2	14	theme	cellulose	295:303	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	14	theme	cellulose	295:303	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	3	15	theme	particle	395:402	arg1	size					404:407	The average particle size	383:407	The average particle size of unmodified MCC	383:425	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	3	15	theme	particle	395:402	arg1	14.208 ± 0.064 μm					437:453	14.208 ± 0.064 μm	437:453	14.208 ± 0.064 μm	437:453	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	2	16	theme	molecular	331:339	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	16	theme	molecular	331:339	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	10	17	theme	allied	1293:1298	arg1	sectors					1300:1306	other food allied sectors	1282:1306	other food allied sectors	1282:1306	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	0	18	theme	Surface	0:6	arg1	modification					8:19	Surface modification	0:19	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.	0:145	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	5	19	theme	significant	657:667	arg1	influence					669:677	an amenable and significant influence	641:677	an amenable and significant influence	641:677	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	2	20	used	used	352:355	arg2	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	20	used	used	352:355	arg2	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	21	theme	different	321:329	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	21	theme	different	321:329	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	0	22	theme	microcrystalline	24:39	arg1	cellulose					41:49	microcrystalline cellulose	24:49	microcrystalline cellulose	24:49	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	0	23	dep	modification	8:19	arg1	applications					89:100	applications	89:100	applications	89:100	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	0	23	dep	modification	8:19	arg1	characterization					68:83	Physicochemical characterization	52:83	Physicochemical characterization	52:83	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	5	24	theme	MCCs	730:733	arg1	stability					717:725	stability	717:725	stability of MCCs	717:733	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	5	24	theme	MCCs	730:733	arg1	properties					702:711	the physicochemical properties	682:711	the physicochemical properties	682:711	It suggested that the molecular weight of active polymer had an amenable and significant influence on the physicochemical properties and stability of MCCs.
30978420	8	25	theme	ionic	1016:1020	arg1	changes					1007:1013	pH changes	1004:1013	pH changes	1004:1013	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	8	25	theme	ionic	1016:1020	arg1	strength					1022:1029	ionic strength	1016:1029	ionic strength	1016:1029	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	2	26	theme	having	314:319	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	26	theme	having	314:319	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	9	27	theme	significant	1114:1124	arg1	role					1126:1129	a significant role	1112:1129	a significant role	1112:1129	It suggested that the re-dispersible MCCs played a significant role in stabilization of emulsions.
30978420	3	28	theme	average	387:393	arg1	size					404:407	The average particle size	383:407	The average particle size of unmodified MCC	383:425	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	3	28	theme	average	387:393	arg1	14.208 ± 0.064 μm					437:453	14.208 ± 0.064 μm	437:453	14.208 ± 0.064 μm	437:453	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	9	29	theme	emulsions	1151:1159	arg1	stabilization					1134:1146	stabilization	1134:1146	stabilization of emulsions	1134:1159	It suggested that the re-dispersible MCCs played a significant role in stabilization of emulsions.
30978420	1	30	theme	microcrystalline	221:236	arg1	MCC					249:251	MCC	249:251	MCC	249:251	The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC).
30978420	1	30	theme	microcrystalline	221:236	arg1	cellulose					238:246	microcrystalline cellulose	221:246	microcrystalline cellulose (MCC)	221:252	The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC).
30978420	3	31	theme	MCC	423:425	arg1	size					404:407	The average particle size	383:407	The average particle size of unmodified MCC	383:425	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	3	31	theme	MCC	423:425	arg1	14.208 ± 0.064 μm					437:453	14.208 ± 0.064 μm	437:453	14.208 ± 0.064 μm	437:453	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	9	32	theme	re-dispersible	1085:1098	arg1	MCCs					1100:1103	the re-dispersible MCCs	1081:1103	the re-dispersible MCCs	1081:1103	It suggested that the re-dispersible MCCs played a significant role in stabilization of emulsions.
30978420	1	33	theme	cellulose	238:246	arg1	surface					210:216	the surface	206:216	the surface of microcrystalline cellulose (MCC)	206:252	The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC).
30978420	0	34	theme	cellulose	41:49	arg1	modification					8:19	Surface modification	0:19	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.	0:145	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	4	35	theme	typical	547:553	arg1	behavior					570:577	a typical shear thinning behavior	545:577	a typical shear thinning behavior	545:577	The modified MCCs exhibited a typical shear thinning behavior.
30978420	0	36	from	applications	89:100	arg1	Stabilization					109:121	the Stabilization	105:121	the Stabilization of Pickering emulsions	105:144	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	10	37	used	used	1274:1277	arg2	MCCs					1194:1197	the re-dispersible MCCs	1175:1197	the re-dispersible MCCs	1175:1197	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	4	38	theme	shear	555:559	arg1	behavior					570:577	a typical shear thinning behavior	545:577	a typical shear thinning behavior	545:577	The modified MCCs exhibited a typical shear thinning behavior.
30978420	10	39	theme	re-dispersible	1179:1192	arg1	MCCs					1194:1197	the re-dispersible MCCs	1175:1197	the re-dispersible MCCs	1175:1197	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	8	40	theme	higher	979:984	arg1	stability					986:994	higher stability	979:994	higher stability	979:994	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	7	41	theme	dried	838:842	arg1	MCCs					844:847	dried MCCs	838:847	dried MCCs	838:847	Moreover, dried MCCs could be re-dispersed in water and could be used as a stabilizer for Pickering emulsions.
30978420	7	41	theme	dried	838:842	arg1	stabilizer					903:912	a stabilizer	901:912	a stabilizer for Pickering emulsions	901:936	Moreover, dried MCCs could be re-dispersed in water and could be used as a stabilizer for Pickering emulsions.
30978420	0	42	theme	Physicochemical	52:66	arg1	characterization					68:83	Physicochemical characterization	52:83	Physicochemical characterization	52:83	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	4	43	theme	thinning	561:568	arg1	behavior					570:577	a typical shear thinning behavior	545:577	a typical shear thinning behavior	545:577	The modified MCCs exhibited a typical shear thinning behavior.
30978420	3	44	theme	unmodified	412:421	arg1	MCC					423:425	unmodified MCC	412:425	unmodified MCC	412:425	The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was increased to 19.576 ± 0.26 μm after modification.
30978420	6	45	theme	molecular	781:789	arg1	weight					791:796	high molecular weight	776:796	high molecular weight	776:796	The composites prepared from CMCNa with high molecular weight were more stable than others.
30978420	7	46	theme	Pickering	918:926	arg1	emulsions					928:936	Pickering emulsions	918:936	Pickering emulsions	918:936	Moreover, dried MCCs could be re-dispersed in water and could be used as a stabilizer for Pickering emulsions.
30978420	0	47	mod	modification	8:19	arg3	Surface					0:6	Surface modification	0:19	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.	0:145	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	0	47	mod	modification	8:19	arg1	cellulose					41:49	microcrystalline cellulose	24:49	microcrystalline cellulose	24:49	Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.
30978420	8	48	theme	pH	1004:1005	arg1	coalescence					1035:1045	coalescence	1035:1045	coalescence	1035:1045	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	8	48	theme	pH	1004:1005	arg1	changes					1007:1013	pH changes	1004:1013	pH changes	1004:1013	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	8	48	theme	pH	1004:1005	arg1	strength					1022:1029	ionic strength	1016:1029	ionic strength	1016:1029	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	2	49	theme	carboxymethyl	281:293	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	49	theme	carboxymethyl	281:293	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	6	50	theme	high	776:779	arg1	weight					791:796	high molecular weight	776:796	high molecular weight	776:796	The composites prepared from CMCNa with high molecular weight were more stable than others.
30978420	10	51	theme	original	1213:1220	arg1	properties					1222:1231	the original properties	1209:1231	the original properties of un-dried composites	1209:1254	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
30978420	8	52	theme	obtained	943:950	arg1	CMCNa-c					963:969	CMCNa-c	963:969	CMCNa-c	963:969	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	8	52	theme	obtained	943:950	arg1	emulsions					952:960	The obtained emulsions	939:960	The obtained emulsions (CMCNa-c)	939:970	The obtained emulsions (CMCNa-c) showed higher stability against pH changes, ionic strength and coalescence during storage.
30978420	2	53	theme	sodium	274:279	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	53	theme	sodium	274:279	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	7	54	used	used	893:896	arg2	MCCs					844:847	dried MCCs	838:847	dried MCCs	838:847	Moreover, dried MCCs could be re-dispersed in water and could be used as a stabilizer for Pickering emulsions.
30978420	7	54	used	used	893:896	arg2	stabilizer					903:912	a stabilizer	901:912	a stabilizer for Pickering emulsions	901:936	Moreover, dried MCCs could be re-dispersed in water and could be used as a stabilizer for Pickering emulsions.
30978420	2	55	theme	polymer	266:272	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	55	theme	polymer	266:272	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	1	56	theme	polymer	165:171	arg1	adsorption					173:182	polymer adsorption	165:182	polymer adsorption	165:182	The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC).
30978420	4	57	theme	modified	521:528	arg1	MCCs					530:533	The modified MCCs	517:533	The modified MCCs	517:533	The modified MCCs exhibited a typical shear thinning behavior.
30978420	2	58	theme	active	259:264	arg1	CMCNa					306:310	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa)	255:311	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	2	58	theme	active	259:264	arg1	weight					341:346	having different molecular weight	314:346	having different molecular weight	314:346	The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs.
30978420	1	59	theme	adsorption	173:182	arg1	phenomenon					151:160	The phenomenon	147:160	The phenomenon of polymer adsorption	147:182	The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC).
30978420	10	60	theme	food	1288:1291	arg1	sectors					1300:1306	other food allied sectors	1282:1306	other food allied sectors	1282:1306	Furthermore, the re-dispersible MCCs preserved the original properties of un-dried composites and could also be used in other food allied sectors.
31252015	0	0	theme	electrospun	107:117	arg1	nanofibers					119:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	3	1	theme	SA-g-AN/PEG	710:720	arg1	solution					731:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	6	2	theme	stable	1348:1353	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	2	theme	stable	1348:1353	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	2	theme	stable	1348:1353	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	2	theme	stable	1348:1353	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	3	3	theme	solution	731:738	arg1	properties					684:693	The physical properties	671:693	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution	671:738	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	4	theme	electron	915:922	arg1	SEM					936:938	SEM	936:938	SEM	936:938	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	4	theme	electron	915:922	arg1	microscopy					924:933	scanning electron microscopy	906:933	scanning electron microscopy (SEM)	906:939	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	5	theme	SA-g-AN	846:852	arg1	morphology					832:841	the morphology	828:841	the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers	828:887	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	0	6	theme	glycol	100:105	arg1	nanofibers					119:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	1	7	dep	acrylonitrile	264:276	arg1	AN					279:280	AN	279:280	AN	279:280	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	8	theme	sodium	176:181	arg1	alginate					183:190	sodium alginate	176:190	sodium alginate	176:190	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	3	9	theme	SA-g-AN	698:704	arg1	solution					731:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	4	10	theme	SA-g-AN	1007:1013	arg1	fibers					1027:1032	SA-g-AN electrospun fibers	1007:1032	SA-g-AN electrospun fibers	1007:1032	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	2	11	theme	chemical	523:530	arg1	structure					532:540	chemical structure	523:540	chemical structure	523:540	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	2	12	theme	infrared	597:604	arg1	FTIR					620:623	FTIR	620:623	FTIR	620:623	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	2	12	theme	infrared	597:604	arg1	spectroscopy					606:617	infrared spectroscopy	597:617	infrared spectroscopy (FTIR)	597:624	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	6	13	theme	uniform	1325:1331	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	13	theme	uniform	1325:1331	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	13	theme	uniform	1325:1331	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	13	theme	uniform	1325:1331	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	4	14	theme	water	987:991	arg1	resistance					993:1002	water resistance	987:1002	water resistance	987:1002	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	2	15	theme	scanning	643:650	arg1	DSC					665:667	DSC	665:667	DSC	665:667	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	2	15	theme	scanning	643:650	arg1	calorimetry					652:662	differential scanning calorimetry	630:662	differential scanning calorimetry (DSC)	630:668	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	1	16	theme	alginate	183:190	arg1	spinnability					160:171	the spinnability	156:171	the spinnability of sodium alginate	156:190	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	3	17	theme	spinning	722:729	arg1	solution					731:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	SA-g-AN and SA-g-AN/PEG spinning solution	698:738	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	2	18	theme	differential	630:641	arg1	DSC					665:667	DSC	665:667	DSC	665:667	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	2	18	theme	differential	630:641	arg1	calorimetry					652:662	differential scanning calorimetry	630:662	differential scanning calorimetry (DSC)	630:668	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	6	19	theme	copolymer	1231:1239	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	19	theme	copolymer	1231:1239	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	19	theme	copolymer	1231:1239	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	19	theme	copolymer	1231:1239	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	4	20	theme	oil-water	950:958	arg1	performance					971:981	the oil-water separation performance	946:981	the oil-water separation performance	946:981	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	3	21	theme	tension	779:785	arg1	meter					787:791	surface tension meter	771:791	surface tension meter	771:791	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	1	22	theme	composite	365:373	arg1	solutions					375:383	SA-g-AN/PEG composite solutions	353:383	SA-g-AN/PEG composite solutions	353:383	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	23	theme	spinning	452:459	arg1	solution					461:468	spinning solution	452:468	spinning solution to prepare corresponding electrospun nanofibers	452:516	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	6	24	theme	graft	1225:1229	arg1	copolymer					1231:1239	modified graft copolymer	1216:1239	modified graft copolymer	1216:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	1	25	theme	potassium	193:201	arg1	initiator					232:240	initiator	232:240	initiator	232:240	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	25	theme	potassium	193:201	arg1	KPS					215:217	KPS	215:217	KPS	215:217	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	25	theme	potassium	193:201	arg1	persulfate					203:212	potassium persulfate	193:212	potassium persulfate (KPS)	193:218	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	2	26	dep	structure	532:540	arg1	The					519:521	The	519:521	The	519:521	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	1	27	used	used	224:227	arg2	KPS					215:217	KPS	215:217	KPS	215:217	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	27	used	used	224:227	arg2	persulfate					203:212	potassium persulfate	193:212	potassium persulfate (KPS)	193:218	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	27	used	used	224:227	arg2	initiator					232:240	initiator	232:240	initiator	232:240	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	6	28	theme	modified	1216:1223	arg1	copolymer					1231:1239	modified graft copolymer	1216:1239	modified graft copolymer	1216:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	3	29	theme	surface	771:777	arg1	meter					787:791	surface tension meter	771:791	surface tension meter	771:791	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	5	30	theme	graft	1089:1093	arg1	modification					1095:1106	the graft modification	1085:1106	the graft modification of acrylonitrile	1085:1123	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	5	31	theme	alginate	1182:1189	arg1	electrospinnability					1152:1170	the electrospinnability	1148:1170	the electrospinnability of sodium alginate	1148:1189	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	6	32	contain	had	1319:1321	arg2	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	32	contain	had	1319:1321	arg2	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	32	contain	had	1319:1321	arg2	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	32	contain	had	1319:1321	arg1	nanofibers					1308:1317	the electrospun nanofibers	1292:1317	the electrospun nanofibers	1292:1317	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	32	contain	had	1319:1321	arg2	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	2	33	theme	thermal	546:552	arg1	stability					554:562	thermal stability	546:562	thermal stability	546:562	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	4	34	theme	fibers	1027:1032	arg1	performance					971:981	the oil-water separation performance	946:981	the oil-water separation performance	946:981	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	4	34	theme	fibers	1027:1032	arg1	resistance					993:1002	water resistance	987:1002	water resistance	987:1002	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	5	35	theme	acrylonitrile	1111:1123	arg1	modification					1095:1106	the graft modification	1085:1106	the graft modification of acrylonitrile	1085:1123	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	6	36	theme	oil-water	1355:1363	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	36	theme	oil-water	1355:1363	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	36	theme	oil-water	1355:1363	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	36	theme	oil-water	1355:1363	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	0	37	theme	alginate	56:63	arg1	characterizations					13:29	characterizations	13:29	characterizations	13:29	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	0	37	theme	alginate	56:63	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	0	37	theme	alginate	56:63	arg1	properties					35:44	properties	35:44	properties	35:44	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	1	38	mod	modified	250:257	arg3	acrylonitrile					264:276	acrylonitrile	264:276	acrylonitrile (AN)	264:281	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	38	mod	modified	250:257	arg1	SA					243:244	SA	243:244	SA	243:244	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	5	39	mod	modification	1095:1106	arg1	acrylonitrile					1111:1123	acrylonitrile	1111:1123	acrylonitrile	1111:1123	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	5	39	mod	modification	1095:1106	arg3	graft					1089:1093	the graft modification	1085:1106	the graft modification of acrylonitrile	1085:1123	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	1	40	theme	corresponding	481:493	arg1	nanofibers					507:516	corresponding electrospun nanofibers	481:516	corresponding electrospun nanofibers	481:516	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	0	41	theme	sodium	49:54	arg1	alginate					56:63	sodium alginate	49:63	sodium alginate	49:63	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	1	42	theme	sodium	294:299	arg1	SA-g-AN					339:345	SA-g-AN	339:345	SA-g-AN	339:345	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	42	theme	sodium	294:299	arg1	copolymer					328:336	sodium alginate-polyacrylonitrile copolymer	294:336	sodium alginate-polyacrylonitrile copolymer (SA-g-AN)	294:346	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	5	43	theme	sodium	1175:1180	arg1	alginate					1182:1189	sodium alginate	1175:1189	sodium alginate	1175:1189	The results revealed that: the graft modification of acrylonitrile significantly improved the electrospinnability of sodium alginate.
31252015	3	44	theme	physical	675:682	arg1	properties					684:693	The physical properties	671:693	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution	671:738	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	1	45	theme	electrospun	495:505	arg1	nanofibers					507:516	corresponding electrospun nanofibers	481:516	corresponding electrospun nanofibers	481:516	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	3	46	theme	fibers	882:887	arg1	morphology					832:841	the morphology	828:841	the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers	828:887	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	1	47	theme	alginate-polyacrylonitrile	301:326	arg1	SA-g-AN					339:345	SA-g-AN	339:345	SA-g-AN	339:345	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	47	theme	alginate-polyacrylonitrile	301:326	arg1	copolymer					328:336	sodium alginate-polyacrylonitrile copolymer	294:336	sodium alginate-polyacrylonitrile copolymer (SA-g-AN)	294:346	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	6	48	theme	separation	1365:1374	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	48	theme	separation	1365:1374	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	48	theme	separation	1365:1374	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	48	theme	separation	1365:1374	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	49	theme	thermal	1269:1275	arg1	performance					1376:1386	stable oil-water separation performance	1348:1386	stable oil-water separation performance	1348:1386	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	49	theme	thermal	1269:1275	arg1	morphology					1333:1342	a uniform morphology	1323:1342	a uniform morphology	1323:1342	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	49	theme	thermal	1269:1275	arg1	water-resistance					1196:1211	The water-resistance	1192:1211	The water-resistance of modified graft copolymer	1192:1239	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	6	49	theme	thermal	1269:1275	arg1	stability					1277:1285	the thermal stability	1265:1285	the thermal stability	1265:1285	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	2	50	theme	SA-g-AN	567:573	arg1	stability					554:562	thermal stability	546:562	thermal stability	546:562	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	2	50	theme	SA-g-AN	567:573	arg1	structure					532:540	chemical structure	523:540	chemical structure	523:540	The chemical structure and thermal stability of SA-g-AN were characterized by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
31252015	4	51	theme	separation	960:969	arg1	performance					971:981	the oil-water separation performance	946:981	the oil-water separation performance	946:981	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	3	52	theme	SA-g-AN/PEG	858:868	arg1	fibers					882:887	SA-g-AN/PEG electrospun fibers	858:887	SA-g-AN/PEG electrospun fibers	858:887	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	0	53	theme	acrylonitrile/polyethylene	73:98	arg1	nanofibers					119:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	acrylonitrile/polyethylene glycol electrospun nanofibers	73:128	Preparation, characterizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nanofibers.
31252015	1	54	theme	polyethylene	423:434	arg1	PEG					444:446	PEG	444:446	PEG	444:446	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	1	54	theme	polyethylene	423:434	arg1	glycol					436:441	polyethylene glycol	423:441	polyethylene glycol (PEG)	423:447	In this work, to improve the spinnability of sodium alginate, potassium persulfate (KPS) was used as initiator, SA was modified with acrylonitrile (AN) to prepare sodium alginate-polyacrylonitrile copolymer (SA-g-AN), and SA-g-AN/PEG composite solutions were prepared by blending SA-g-AN and polyethylene glycol (PEG) as spinning solution to prepare corresponding electrospun nanofibers.
31252015	3	55	theme	electrospun	870:880	arg1	fibers					882:887	SA-g-AN/PEG electrospun fibers	858:887	SA-g-AN/PEG electrospun fibers	858:887	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	56	theme	conductivity	808:819	arg1	meter					821:825	electrical conductivity meter	797:825	electrical conductivity meter	797:825	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	4	57	theme	electrospun	1015:1025	arg1	fibers					1027:1032	SA-g-AN electrospun fibers	1007:1032	SA-g-AN electrospun fibers	1007:1032	And the oil-water separation performance and water resistance of SA-g-AN electrospun fibers were also investigated.
31252015	6	58	theme	electrospun	1296:1306	arg1	nanofibers					1308:1317	the electrospun nanofibers	1292:1317	the electrospun nanofibers	1292:1317	The water-resistance of modified graft copolymer was improved as well as the thermal stability, and the electrospun nanofibers had a uniform morphology and stable oil-water separation performance.
31252015	3	59	theme	scanning	906:913	arg1	SEM					936:938	SEM	936:938	SEM	936:938	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	59	theme	scanning	906:913	arg1	microscopy					924:933	scanning electron microscopy	906:933	scanning electron microscopy (SEM)	906:939	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
31252015	3	60	theme	electrical	797:806	arg1	meter					821:825	electrical conductivity meter	797:825	electrical conductivity meter	797:825	The physical properties of SA-g-AN and SA-g-AN/PEG spinning solution were determined by viscometer, surface tension meter and electrical conductivity meter, the morphology of SA-g-AN and SA-g-AN/PEG electrospun fibers were observed by scanning electron microscopy (SEM).
30310109	5	0	theme	screening	927:935	arg1	effort					937:942	the automated screening effort	913:942	the automated screening effort	913:942	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	1	1	gly	glycosylation	83:95	arg1	flavonoids					100:109	flavonoids	100:109	flavonoids	100:109	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	6	2	theme	considered	1119:1128	arg1	priori					1112:1117	a priori considered as recalcitrant compounds to glucosylation	1110:1171	a priori considered as recalcitrant compounds to glucosylation using this α-transglucosylases	1110:1202	Notably, naringenin and morin, a priori considered as recalcitrant compounds to glucosylation using this α-transglucosylases, could also be modified.
30310109	6	2	theme	considered	1119:1128	arg1	naringenin					1088:1097	naringenin	1088:1097	naringenin	1088:1097	Notably, naringenin and morin, a priori considered as recalcitrant compounds to glucosylation using this α-transglucosylases, could also be modified.
30310109	2	3	theme	enzymatic	345:353	arg1	platform					355:362	a valuable enzymatic platform	334:362	a valuable enzymatic platform for flavonoid glucosylation	334:390	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	7	4	theme	molecular	1376:1384	arg1	structures					1386:1395	molecular structures	1376:1395	molecular structures inaccessible yet	1376:1412	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	3	5	theme	pair-wise	561:569	arg1	mutations					571:579	pair-wise mutations	561:579	pair-wise mutations inferred by molecular docking simulations	561:621	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	1	6	dep	degradation	160:170	arg1	enhancing					178:186	enhancing	178:186	enhancing their solubility	178:203	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	3	7	theme	molecular	593:601	arg1	simulations					611:621	molecular docking simulations	593:621	molecular docking simulations	593:621	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	1	8	theme	Enzymatic	73:81	arg1	glycosylation					83:95	Enzymatic glycosylation	73:95	Enzymatic glycosylation of flavonoids	73:109	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	1	8	theme	Enzymatic	73:81	arg1	mean					127:130	an efficient mean	114:130	an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care	114:319	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	6	9	theme	recalcitrant	1133:1144	arg1	compounds					1146:1154	recalcitrant compounds	1133:1154	recalcitrant compounds	1133:1154	Notably, naringenin and morin, a priori considered as recalcitrant compounds to glucosylation using this α-transglucosylases, could also be modified.
30310109	5	10	theme	other	865:869	arg1	flavonoids					871:880	five other flavonoids	860:880	five other flavonoids	860:880	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	1	11	dep	mean	127:130	arg1	protect					135:141	protect	135:141	to protect aglycons against degradation while enhancing their solubility, life time	132:214	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	2	12	theme	α-1,2	396:400	arg1	sucrase					412:418	an α-1,2 branching sucrase	393:418	an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases	393:469	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	2	12	theme	α-1,2	396:400	arg1	template					487:494	template	487:494	template	487:494	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	0	13	theme	glucoside	46:54	arg1	diversification					56:70	flavonoid glucoside diversification	36:70	flavonoid glucoside diversification	36:70	Engineering a branching sucrase for flavonoid glucoside diversification.
30310109	1	14	from	applications	293:304	arg1	care					316:319	health care	309:319	health care	309:319	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	7	15	theme	flavonoid	1352:1360	arg1	glucosides					1362:1371	flavonoid glucosides	1352:1371	flavonoid glucosides	1352:1371	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	4	16	theme	quercetin	783:791	arg1	conversion					793:802	quercetin conversion	783:802	quercetin conversion	783:802	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	5	17	with	luteolin	993:1000	arg1	conversion					1043:1052	conversion	1043:1052	conversion ranging from 30% to 90%	1043:1076	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	1	18	theme	flavonoids	100:109	arg1	glycosylation					83:95	Enzymatic glycosylation	73:95	Enzymatic glycosylation of flavonoids	73:109	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	1	18	theme	flavonoids	100:109	arg1	mean					127:130	an efficient mean	114:130	an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care	114:319	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	7	19	theme	glucosides	1362:1371	arg1	diversity					1339:1347	the diversity	1335:1347	the diversity of flavonoid glucosides to molecular structures inaccessible yet	1335:1412	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	5	20	theme	acceptor	831:838	arg1	promiscuity					840:850	Their acceptor promiscuity	825:850	Their acceptor promiscuity towards five other flavonoids	825:880	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	3	21	theme	docking	603:609	arg1	simulations					611:621	molecular docking simulations	593:621	molecular docking simulations	593:621	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	1	22	theme	life	206:209	arg1	degradation					160:170	degradation	160:170	degradation while enhancing their solubility	160:203	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	1	22	theme	life	206:209	arg1	time					211:214	life time	206:214	life time	206:214	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	7	23	dep	structures	1386:1395	arg1	inaccessible					1397:1408	inaccessible	1397:1408	inaccessible	1397:1408	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	0	24	theme	branching	14:22	arg1	sucrase					24:30	a branching sucrase	12:30	a branching sucrase for flavonoid glucoside diversification	12:70	Engineering a branching sucrase for flavonoid glucoside diversification.
30310109	3	25	theme	variants	542:549	arg1	libraries					529:537	Two libraries	525:537	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations	525:621	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	1	26	theme	efficient	117:125	arg1	glycosylation					83:95	Enzymatic glycosylation	73:95	Enzymatic glycosylation of flavonoids	73:109	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	1	26	theme	efficient	117:125	arg1	mean					127:130	an efficient mean	114:130	an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care	114:319	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	4	27	theme	limited	721:727	arg1	number					729:734	Only a limited number	714:734	Only a limited number of variants (22)	714:751	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	0	28	theme	flavonoid	36:44	arg1	diversification					56:70	flavonoid glucoside diversification	36:70	flavonoid glucoside diversification	36:70	Engineering a branching sucrase for flavonoid glucoside diversification.
30310109	7	29	theme	engineered	1286:1295	arg1	α-transglucosylases					1302:1320	engineered GH70 α-transglucosylases	1286:1320	engineered GH70 α-transglucosylases	1286:1320	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	5	30	theme	30	1067:1068	arg1	%					1069:1069	%	1069:1069	%	1069:1069	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	5	31	dep	%	1069:1069	arg1	to					1071:1072	to	1071:1072	to	1071:1072	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	5	32	with	glucosylation	1024:1036	arg1	conversion					1043:1052	conversion	1043:1052	conversion ranging from 30% to 90%	1043:1076	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	2	33	theme	branching	402:410	arg1	sucrase					412:418	an α-1,2 branching sucrase	393:418	an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases	393:469	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	2	33	theme	branching	402:410	arg1	template					487:494	template	487:494	template	487:494	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	5	34	with	morin	1003:1007	arg1	conversion					1043:1052	conversion	1043:1052	conversion ranging from 30% to 90%	1043:1076	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	1	35	theme	health	309:314	arg1	care					316:319	health care	309:319	health care	309:319	Enzymatic glycosylation of flavonoids is an efficient mean to protect aglycons against degradation while enhancing their solubility, life time and, by extension, their bioavailability which is critical for most of their applications in health care.
30310109	2	36	theme	valuable	336:343	arg1	platform					355:362	a valuable enzymatic platform	334:362	a valuable enzymatic platform for flavonoid glucosylation	334:390	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	2	37	theme	glycoside-hydrolases	450:469	arg1	family					437:442	the family 70	433:445	the family 70 of glycoside-hydrolases	433:469	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	5	38	theme	90	1074:1075	arg1	%					1069:1069	%	1069:1069	%	1069:1069	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	4	39	theme	variants	739:746	arg1	number					729:734	Only a limited number	714:734	Only a limited number of variants (22)	714:751	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	7	40	theme	GH70	1297:1300	arg1	α-transglucosylases					1302:1320	engineered GH70 α-transglucosylases	1286:1320	engineered GH70 α-transglucosylases	1286:1320	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	4	41	theme	product	808:814	arg1	profile					816:822	product profile	808:822	product profile	808:822	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	3	42	theme	quercetin	655:663	arg1	glucosylation					665:677	quercetin glucosylation	655:677	quercetin glucosylation	655:677	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	7	43	theme	α-transglucosylases	1302:1320	arg1	platforms					1273:1281	small platforms	1267:1281	small platforms of engineered GH70 α-transglucosylases	1267:1320	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	4	44	theme	conversion	793:802	arg1	basis					774:778	the basis	770:778	the basis of quercetin conversion and product profile	770:822	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	3	45	theme	glucosyl	698:705	arg1	donor					707:711	a glucosyl donor	696:711	a glucosyl donor	696:711	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	3	45	theme	glucosyl	698:705	arg1	sucrose					685:691	sucrose	685:691	sucrose	685:691	Two libraries of variants targeting pair-wise mutations inferred by molecular docking simulations were generated and screened for quercetin glucosylation using sucrose as a glucosyl donor.
30310109	5	46	theme	remarkable	970:979	arg1	ability					981:987	remarkable ability	970:987	remarkable ability	970:987	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	5	47	theme	naringenin	1013:1022	arg1	glucosylation					1024:1036	naringenin glucosylation	1013:1036	naringenin glucosylation	1013:1036	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	2	48	theme	flavonoid	368:376	arg1	glucosylation					378:390	flavonoid glucosylation	368:390	flavonoid glucosylation	368:390	To generate a valuable enzymatic platform for flavonoid glucosylation, an α-1,2 branching sucrase belonging to the family 70 of glycoside-hydrolases was selected as template and subsequently engineered.
30310109	7	49	theme	small	1267:1271	arg1	platforms					1273:1281	small platforms	1267:1281	small platforms of engineered GH70 α-transglucosylases	1267:1320	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30310109	4	50	theme	profile	816:822	arg1	basis					774:778	the basis	770:778	the basis of quercetin conversion and product profile	770:822	Only a limited number of variants (22) were retained on the basis of quercetin conversion and product profile.
30310109	5	51	theme	automated	917:925	arg1	effort					937:942	the automated screening effort	913:942	the automated screening effort	913:942	Their acceptor promiscuity towards five other flavonoids was subsequently assessed, and the automated screening effort revealed variants showing remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%.
30310109	7	52	theme	platforms	1273:1281	arg1	potential					1254:1262	the potential	1250:1262	the potential of small platforms of engineered GH70 α-transglucosylases	1250:1320	The approach reveals the potential of small platforms of engineered GH70 α-transglucosylases and opens up the diversity of flavonoid glucosides to molecular structures inaccessible yet.
30543887	5	0	theme	X-ray	682:686	arg1	XRD					710:712	XRD	710:712	XRD	710:712	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	5	0	theme	X-ray	682:686	arg1	analysis					700:707	X-ray diffraction analysis	682:707	X-ray diffraction analysis (XRD)	682:713	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	6	1	theme	micro-dilution	835:848	arg1	n = 3					857:861	n = 3	857:861	n = 3	857:861	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	1	theme	micro-dilution	835:848	arg1	assay					850:854	broth micro-dilution assay	829:854	broth micro-dilution assay (n = 3)	829:862	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	8	2	theme	tissue	1164:1169	arg1	group					1143:1147	a control group	1133:1147	a control group (chitosan-free tissue conditioner)	1133:1182	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	2	theme	tissue	1164:1169	arg1	conditioner					1171:1181	chitosan-free tissue conditioner	1150:1181	chitosan-free tissue conditioner	1150:1181	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	11	3	theme	pure	1512:1515	arg1	chitosan					1528:1535	chitosan	1528:1535	chitosan	1528:1535	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	12	4	from	reduction	1652:1660	arg1	number					1665:1670	number	1665:1670	number of CFUs of C. albicans density	1665:1701	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	10	5	theme	in-vitro	1403:1410	arg1	activity					1423:1430	A potent in-vitro antifungal activity	1394:1430	A potent in-vitro antifungal activity against C. albicans	1394:1450	A potent in-vitro antifungal activity against C. albicans was observed.
30543887	5	6	theme	chitosan	522:529	arg1	COS					548:550	COS	548:550	COS	548:550	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	5	6	theme	chitosan	522:529	arg1	oligosaccharide					531:545	A chitosan oligosaccharide	520:545	A chitosan oligosaccharide (COS)	520:551	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	1	7	theme	ill-fitting	193:203	arg1	dentures					205:212	ill-fitting dentures	193:212	ill-fitting dentures	193:212	Tissue conditioners are commonly used to improve the adaptation of ill-fitting dentures.
30543887	1	8	used	used	159:162	arg2	conditioners					133:144	Tissue conditioners	126:144	Tissue conditioners	126:144	Tissue conditioners are commonly used to improve the adaptation of ill-fitting dentures.
30543887	11	9	theme	COS	1541:1543	arg1	MIC					1504:1506	MIC	1504:1506	MIC	1504:1506	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	9	theme	COS	1541:1543	arg1	concentration					1489:1501	The minimum inhibitory concentration	1466:1501	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS	1466:1543	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	9	theme	COS	1541:1543	arg1	0.625 mg/ml					1549:1559	0.625 mg/ml	1549:1559	0.625 mg/ml	1549:1559	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	15	10	theme	denture	2136:2142	arg1	stomatitis					2144:2153	denture stomatitis	2136:2153	denture stomatitis	2136:2153	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	11	11	theme	chitosan	1528:1535	arg1	MIC					1504:1506	MIC	1504:1506	MIC	1504:1506	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	11	theme	chitosan	1528:1535	arg1	concentration					1489:1501	The minimum inhibitory concentration	1466:1501	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS	1466:1543	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	11	theme	chitosan	1528:1535	arg1	0.625 mg/ml					1549:1559	0.625 mg/ml	1549:1559	0.625 mg/ml	1549:1559	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	2	12	theme	denture	301:307	arg1	"					319:319	"denture stomatitis"	300:319	"denture stomatitis"	300:319	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	8	13	theme	forming	1214:1220	arg1	CFUs					1229:1232	CFUs	1229:1232	CFUs	1229:1232	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	13	theme	forming	1214:1220	arg1	n = 3					1236:1240	n = 3	1236:1240	n = 3	1236:1240	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	13	theme	forming	1214:1220	arg1	units					1222:1226	colony forming units	1207:1226	colony forming units (CFUs) (n = 3)	1207:1241	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	12	14	theme	control	1608:1614	arg1	group					1616:1620	the control group	1604:1620	the control group	1604:1620	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	2	15	theme	Candida	255:261	arg1	albicans					263:270	Candida albicans	255:270	Candida albicans (C. albicans)	255:284	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	2	15	theme	Candida	255:261	arg1	albicans					276:283	C. albicans	273:283	C. albicans	273:283	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	9	16	from	potential	1259:1267	arg1	environment					1328:1338	a simulated oral environment	1311:1338	a simulated oral environment	1311:1338	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	14	17	from	reduction	1951:1959	arg1	density					1976:1982	C. albicans density	1964:1982	C. albicans density	1964:1982	Experimental formulations showed a reduction in C. albicans density compared to the control group.
30543887	12	18	theme	density	1695:1701	arg1	CFUs					1675:1678	CFUs	1675:1678	CFUs of C. albicans density	1675:1701	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	4	19	theme	tissue	434:439	arg1	conditioner					441:451	a chitosan-based tissue conditioner	417:451	a chitosan-based tissue conditioner	417:451	This study aims to formulate a chitosan-based tissue conditioner and assess its anti-fungal efficacy against C. albicans over time.
30543887	11	20	theme	minimum	1470:1476	arg1	MIC					1504:1506	MIC	1504:1506	MIC	1504:1506	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	20	theme	minimum	1470:1476	arg1	concentration					1489:1501	The minimum inhibitory concentration	1466:1501	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS	1466:1543	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	20	theme	minimum	1470:1476	arg1	0.625 mg/ml					1549:1559	0.625 mg/ml	1549:1559	0.625 mg/ml	1549:1559	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	0	21	theme	potential	74:82	arg1	therapy					94:100	potential treatment therapy	74:100	potential treatment therapy for denture stomatitis	74:123	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	12	22	theme	CFUs	1675:1678	arg1	number					1665:1670	number	1665:1670	number of CFUs of C. albicans density	1665:1701	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	10	23	theme	C.	1440:1441	arg1	albicans					1443:1450	C. albicans	1440:1450	C. albicans	1440:1450	A potent in-vitro antifungal activity against C. albicans was observed.
30543887	9	24	theme	experimental	1272:1283	arg1	formulations					1285:1296	experimental formulations	1272:1296	experimental formulations over time	1272:1306	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	12	25	theme	C.	1683:1684	arg1	density					1695:1701	C. albicans density	1683:1701	C. albicans density	1683:1701	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	3	26	theme	antifungal	367:376	arg1	activity					378:385	inherent antifungal activity	358:385	inherent antifungal activity	358:385	Chitosan and its derivative possess inherent antifungal activity.
30543887	7	27	dep	conditioner	956:966	arg1	i.e.					944:947	i.e.	944:947	i.e.	944:947	Next, it was incorporated into tissue conditioners and two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS) were prepared.
30543887	0	28	theme	denture	106:112	arg1	stomatitis					114:123	denture stomatitis	106:123	denture stomatitis	106:123	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	13	29	from	reduction	1867:1875	arg1	effect					1895:1900	the inhibitory effect	1880:1900	the inhibitory effect	1880:1900	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	4	30	theme	C.	497:498	arg1	albicans					500:507	C. albicans	497:507	C. albicans	497:507	This study aims to formulate a chitosan-based tissue conditioner and assess its anti-fungal efficacy against C. albicans over time.
30543887	6	31	theme	inhibitory	728:737	arg1	MIC					754:756	MIC	754:756	MIC	754:756	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	31	theme	inhibitory	728:737	arg1	concentration					739:751	The minimum inhibitory concentration	716:751	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans	716:807	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	7	32	theme	tissue	949:954	arg1	conditioner					956:966	tissue conditioner	949:966	tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS)	949:1043	Next, it was incorporated into tissue conditioners and two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS) were prepared.
30543887	15	33	theme	novel	2023:2027	arg1	combination					2029:2039	a novel combination	2021:2039	a novel combination of chitosan and tissue conditioners	2021:2075	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	15	33	theme	novel	2023:2027	arg1	alternative					2092:2102	a promising alternative	2080:2102	a promising alternative for prevention and treatment of denture stomatitis	2080:2153	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	0	34	theme	In-vitro	0:7	arg1	efficacy					20:27	In-vitro antifungal efficacy	0:27	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.	0:124	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	9	35	theme	simulated	1313:1321	arg1	environment					1328:1338	a simulated oral environment	1311:1338	a simulated oral environment	1311:1338	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	12	36	theme	experimental	1623:1634	arg1	groups					1636:1641	experimental groups	1623:1641	experimental groups	1623:1641	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	8	37	theme	antifungal	1073:1082	arg1	activity					1084:1091	The in-vitro antifungal activity	1060:1091	The in-vitro antifungal activity of experimental group	1060:1113	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	12	38	dep	C.	1683:1684	arg1	albicans					1686:1693	albicans	1686:1693	albicans	1686:1693	Compared to the control group, experimental groups showed a reduction in number of CFUs of C. albicans density.
30543887	1	39	theme	Tissue	126:131	arg1	conditioners					133:144	Tissue conditioners	126:144	Tissue conditioners	126:144	Tissue conditioners are commonly used to improve the adaptation of ill-fitting dentures.
30543887	0	40	theme	tissue	32:37	arg1	composites					60:69	tissue conditioner-chitosan composites	32:69	tissue conditioner-chitosan composites	32:69	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	6	41	theme	commercial	770:779	arg1	chitosan					781:788	commercial chitosan	770:788	commercial chitosan	770:788	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	0	42	theme	composites	60:69	arg1	efficacy					20:27	In-vitro antifungal efficacy	0:27	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.	0:124	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	8	43	theme	group	1109:1113	arg1	activity					1084:1091	The in-vitro antifungal activity	1060:1091	The in-vitro antifungal activity of experimental group	1060:1113	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	6	44	theme	COS	762:764	arg1	MIC					754:756	MIC	754:756	MIC	754:756	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	44	theme	COS	762:764	arg1	concentration					739:751	The minimum inhibitory concentration	716:751	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans	716:807	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	7	45	theme	experimental	924:935	arg1	groups					937:942	two experimental groups	920:942	two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS)	920:1043	Next, it was incorporated into tissue conditioners and two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS) were prepared.
30543887	15	46	theme	conditioners	2064:2075	arg1	combination					2029:2039	a novel combination	2021:2039	a novel combination of chitosan and tissue conditioners	2021:2075	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	15	46	theme	conditioners	2064:2075	arg1	alternative					2092:2102	a promising alternative	2080:2102	a promising alternative for prevention and treatment of denture stomatitis	2080:2153	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	7	47	theme	tissue	896:901	arg1	conditioners					903:914	tissue conditioners	896:914	tissue conditioners	896:914	Next, it was incorporated into tissue conditioners and two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS) were prepared.
30543887	13	48	theme	inhibitory	1752:1761	arg1	effect					1763:1768	its inhibitory effect	1748:1768	its inhibitory effect	1748:1768	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	5	49	theme	diffraction	688:698	arg1	XRD					710:712	XRD	710:712	XRD	710:712	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	5	49	theme	diffraction	688:698	arg1	analysis					700:707	X-ray diffraction analysis	682:707	X-ray diffraction analysis (XRD)	682:713	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	6	50	theme	broth	829:833	arg1	n = 3					857:861	n = 3	857:861	n = 3	857:861	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	50	theme	broth	829:833	arg1	assay					850:854	broth micro-dilution assay	829:854	broth micro-dilution assay (n = 3)	829:862	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	14	51	dep	C.	1964:1965	arg1	albicans					1967:1974	albicans	1967:1974	albicans	1967:1974	Experimental formulations showed a reduction in C. albicans density compared to the control group.
30543887	8	52	theme	chitosan-free	1150:1162	arg1	group					1143:1147	a control group	1133:1147	a control group (chitosan-free tissue conditioner)	1133:1182	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	52	theme	chitosan-free	1150:1162	arg1	conditioner					1171:1181	chitosan-free tissue conditioner	1150:1181	chitosan-free tissue conditioner	1150:1181	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	4	53	theme	chitosan-based	419:432	arg1	conditioner					441:451	a chitosan-based tissue conditioner	417:451	a chitosan-based tissue conditioner	417:451	This study aims to formulate a chitosan-based tissue conditioner and assess its anti-fungal efficacy against C. albicans over time.
30543887	11	54	theme	commercial	1517:1526	arg1	chitosan					1528:1535	chitosan	1528:1535	chitosan	1528:1535	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	15	55	theme	stomatitis	2144:2153	arg1	treatment					2123:2131	treatment	2123:2131	treatment	2123:2131	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	15	55	theme	stomatitis	2144:2153	arg1	prevention					2108:2117	prevention	2108:2117	prevention	2108:2117	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	10	56	theme	potent	1396:1401	arg1	activity					1423:1430	A potent in-vitro antifungal activity	1394:1430	A potent in-vitro antifungal activity against C. albicans	1394:1450	A potent in-vitro antifungal activity against C. albicans was observed.
30543887	1	57	theme	dentures	205:212	arg1	adaptation					179:188	the adaptation	175:188	the adaptation of ill-fitting dentures	175:212	Tissue conditioners are commonly used to improve the adaptation of ill-fitting dentures.
30543887	2	58	theme	stomatitis	309:318	arg1	"					319:319	"denture stomatitis"	300:319	"denture stomatitis"	300:319	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	4	59	theme	anti-fungal	468:478	arg1	efficacy					480:487	its anti-fungal efficacy	464:487	its anti-fungal efficacy against C. albicans over time	464:517	This study aims to formulate a chitosan-based tissue conditioner and assess its anti-fungal efficacy against C. albicans over time.
30543887	8	60	theme	colony	1207:1212	arg1	CFUs					1229:1232	CFUs	1229:1232	CFUs	1229:1232	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	60	theme	colony	1207:1212	arg1	n = 3					1236:1240	n = 3	1236:1240	n = 3	1236:1240	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	60	theme	colony	1207:1212	arg1	units					1222:1226	colony forming units	1207:1226	colony forming units (CFUs) (n = 3)	1207:1241	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	61	theme	units	1222:1226	arg1	numbers					1196:1202	numbers	1196:1202	numbers of colony forming units (CFUs) (n = 3)	1196:1241	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	11	62	theme	inhibitory	1478:1487	arg1	MIC					1504:1506	MIC	1504:1506	MIC	1504:1506	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	62	theme	inhibitory	1478:1487	arg1	concentration					1489:1501	The minimum inhibitory concentration	1466:1501	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS	1466:1543	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	11	62	theme	inhibitory	1478:1487	arg1	0.625 mg/ml					1549:1559	0.625 mg/ml	1549:1559	0.625 mg/ml	1549:1559	The minimum inhibitory concentration (MIC) of pure commercial chitosan and COS was 0.625 mg/ml and 0.3125 mg/ml respectively.
30543887	15	63	theme	tissue	2057:2062	arg1	conditioners					2064:2075	tissue conditioners	2057:2075	tissue conditioners	2057:2075	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	5	64	theme	purchased	587:595	arg1	chitosan					597:604	commercially purchased chitosan	574:604	commercially purchased chitosan	574:604	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	13	65	theme	inhibitory	1884:1893	arg1	effect					1895:1900	the inhibitory effect	1880:1900	the inhibitory effect	1880:1900	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	0	66	theme	treatment	84:92	arg1	therapy					94:100	potential treatment therapy	74:100	potential treatment therapy for denture stomatitis	74:123	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	9	67	theme	antifungal	1248:1257	arg1	potential					1259:1267	The antifungal potential	1244:1267	The antifungal potential of experimental formulations over time in a simulated oral environment	1244:1338	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	2	68	theme	C.	273:274	arg1	albicans					263:270	Candida albicans	255:270	Candida albicans (C. albicans)	255:284	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	2	68	theme	C.	273:274	arg1	albicans					276:283	C. albicans	273:283	C. albicans	273:283	These materials are easily colonized by Candida albicans (C. albicans), resulting in "denture stomatitis".
30543887	7	69	theme	tissue	1001:1006	arg1	conditioner					1008:1018	tissue conditioner	1001:1018	tissue conditioner modified by COS (TC-COS)	1001:1043	Next, it was incorporated into tissue conditioners and two experimental groups i.e. tissue conditioner modified by chitosan (TC-CH) and tissue conditioner modified by COS (TC-COS) were prepared.
30543887	13	70	theme	gradual	1859:1865	arg1	reduction					1867:1875	a gradual reduction	1857:1875	a gradual reduction in the inhibitory effect	1857:1900	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	9	71	theme	formulations	1285:1296	arg1	potential					1259:1267	The antifungal potential	1244:1267	The antifungal potential of experimental formulations over time in a simulated oral environment	1244:1338	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	14	72	theme	control	2000:2006	arg1	group					2008:2012	the control group	1996:2012	the control group	1996:2012	Experimental formulations showed a reduction in C. albicans density compared to the control group.
30543887	10	73	theme	antifungal	1412:1421	arg1	activity					1423:1430	A potent in-vitro antifungal activity	1394:1430	A potent in-vitro antifungal activity against C. albicans	1394:1450	A potent in-vitro antifungal activity against C. albicans was observed.
30543887	13	74	theme	third	1833:1837	arg1	day					1839:1841	the third day	1829:1841	the third day	1829:1841	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	5	75	theme	Infrared	649:656	arg1	FTIR					672:675	FTIR	672:675	FTIR	672:675	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	5	75	theme	Infrared	649:656	arg1	spectroscopy					658:669	Infrared spectroscopy	649:669	Infrared spectroscopy (FTIR)	649:676	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
30543887	3	76	theme	inherent	358:365	arg1	activity					378:385	inherent antifungal activity	358:385	inherent antifungal activity	358:385	Chitosan and its derivative possess inherent antifungal activity.
30543887	6	77	theme	minimum	720:726	arg1	MIC					754:756	MIC	754:756	MIC	754:756	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	77	theme	minimum	720:726	arg1	concentration					739:751	The minimum inhibitory concentration	716:751	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans	716:807	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	0	78	theme	antifungal	9:18	arg1	efficacy					20:27	In-vitro antifungal efficacy	0:27	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.	0:124	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	8	79	theme	in-vitro	1064:1071	arg1	activity					1084:1091	The in-vitro antifungal activity	1060:1091	The in-vitro antifungal activity of experimental group	1060:1113	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	13	80	theme	improved	1803:1810	arg1	inhibition					1812:1821	improved inhibition	1803:1821	improved inhibition	1803:1821	Once immersed in saliva, the TC-CH retained its inhibitory effect for 24 h, while TC-COS exhibited improved inhibition until the third day, beyond which a gradual reduction in the inhibitory effect was observed.
30543887	9	81	theme	oral	1323:1326	arg1	environment					1328:1338	a simulated oral environment	1311:1338	a simulated oral environment	1311:1338	The antifungal potential of experimental formulations over time in a simulated oral environment was also evaluated using a spectrophotometry (n = 3).
30543887	14	82	theme	C.	1964:1965	arg1	density					1976:1982	C. albicans density	1964:1982	C. albicans density	1964:1982	Experimental formulations showed a reduction in C. albicans density compared to the control group.
30543887	0	83	theme	conditioner-chitosan	39:58	arg1	composites					60:69	tissue conditioner-chitosan composites	32:69	tissue conditioner-chitosan composites	32:69	In-vitro antifungal efficacy of tissue conditioner-chitosan composites as potential treatment therapy for denture stomatitis.
30543887	8	84	theme	experimental	1096:1107	arg1	group					1109:1113	experimental group	1096:1113	experimental group	1096:1113	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	15	85	theme	promising	2082:2090	arg1	combination					2029:2039	a novel combination	2021:2039	a novel combination of chitosan and tissue conditioners	2021:2075	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	15	85	theme	promising	2082:2090	arg1	alternative					2092:2102	a promising alternative	2080:2102	a promising alternative for prevention and treatment of denture stomatitis	2080:2153	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	6	86	theme	chitosan	781:788	arg1	MIC					754:756	MIC	754:756	MIC	754:756	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	6	86	theme	chitosan	781:788	arg1	concentration					739:751	The minimum inhibitory concentration	716:751	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans	716:807	The minimum inhibitory concentration (MIC) of COS and commercial chitosan against C. abicans was evaluated using broth micro-dilution assay (n = 3).
30543887	3	87	contain	possess	350:356	arg2	activity					378:385	inherent antifungal activity	358:385	inherent antifungal activity	358:385	Chitosan and its derivative possess inherent antifungal activity.
30543887	3	87	contain	possess	350:356	arg1	Chitosan					322:329	Chitosan	322:329	Chitosan	322:329	Chitosan and its derivative possess inherent antifungal activity.
30543887	3	87	contain	possess	350:356	arg1	derivative					339:348	its derivative	335:348	its derivative	335:348	Chitosan and its derivative possess inherent antifungal activity.
30543887	14	88	theme	Experimental	1916:1927	arg1	formulations					1929:1940	Experimental formulations	1916:1940	Experimental formulations	1916:1940	Experimental formulations showed a reduction in C. albicans density compared to the control group.
30543887	8	89	theme	control	1135:1141	arg1	group					1143:1147	a control group	1133:1147	a control group (chitosan-free tissue conditioner)	1133:1182	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	8	89	theme	control	1135:1141	arg1	conditioner					1171:1181	chitosan-free tissue conditioner	1150:1181	chitosan-free tissue conditioner	1150:1181	The in-vitro antifungal activity of experimental group was compared with a control group (chitosan-free tissue conditioner) by counting numbers of colony forming units (CFUs) (n = 3).
30543887	15	90	theme	chitosan	2044:2051	arg1	combination					2029:2039	a novel combination	2021:2039	a novel combination of chitosan and tissue conditioners	2021:2075	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	15	90	theme	chitosan	2044:2051	arg1	alternative					2092:2102	a promising alternative	2080:2102	a promising alternative for prevention and treatment of denture stomatitis	2080:2153	Thus, a novel combination of chitosan and tissue conditioners is a promising alternative for prevention and treatment of denture stomatitis.
30543887	5	91	dep	Fourier	631:637	arg1	Transform					639:647	Transform	639:647	Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD)	639:713	A chitosan oligosaccharide (COS) was synthesized from commercially purchased chitosan and was characterized by Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD).
31439876	5	0	contain	contained	884:892	arg1	formation					856:864	the formation	852:864	the formation	852:864	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	5	0	contain	contained	884:892	arg2	exopolysaccharide					902:918	a novel exopolysaccharide	894:918	a novel exopolysaccharide consisting of an amylose-like glucan	894:955	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	3	1	dep	15-fold	583:589	arg1	to					580:581	to	580:581	to	580:581	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	1	dep	15-fold	583:589	arg1	more					591:594	more	591:594	more	591:594	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	7	2	attach	attached	1127:1134	arg2	novicida					1118:1125	F. novicida	1115:1125	F. novicida	1115:1125	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	7	2	attach	attached	1127:1134	arg1	surfaces					1139:1146	surfaces	1139:1146	surfaces	1139:1146	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	6	3	theme	bacterium	1017:1025	arg1	composition					998:1008	the surface polysaccharide composition	971:1008	the surface polysaccharide composition of the bacterium	971:1025	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	0	4	theme	Surface	68:74	arg1	Glycosylation					76:88	Cell Surface Glycosylation	63:88	Cell Surface Glycosylation	63:88	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	5	5	theme	novel	896:900	arg1	exopolysaccharide					902:918	a novel exopolysaccharide	894:918	a novel exopolysaccharide consisting of an amylose-like glucan	894:955	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	3	6	theme	O-antigen	526:534	arg1	capsule					536:542	O-antigen capsule	526:542	O-antigen capsule	526:542	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	0	7	theme	Growth	91:96	arg1	Medium					98:103	Growth Medium	91:103	Growth Medium	91:103	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	7	8	theme	F.	1115:1116	arg1	novicida					1118:1125	F. novicida	1115:1125	F. novicida	1115:1125	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	7	9	theme	F.	1297:1298	arg1	formation					1317:1325	F. novicida biofilm formation	1297:1325	F. novicida biofilm formation	1297:1325	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	8	10	theme	surface	1372:1378	arg1	polysaccharides					1380:1394	surface polysaccharides	1372:1394	surface polysaccharides	1372:1394	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	11	dep	F.	1505:1506	arg1	tularensis					1508:1517	F. tularensis	1505:1517	F. tularensis	1505:1517	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	7	12	theme	A	1206:1206	arg1	strains					1213:1219	Type A or B strains	1201:1219	Type A or B strains	1201:1219	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	3	13	dep	F.	414:415	arg1	tularensis					417:426	F. tularensis	414:426	F. tularensis Types A and B	414:440	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	4	14	from	complex	778:784	arg1	deficient					738:746	deficient	738:746	deficient	738:746	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	15	theme	capsule-like	765:776	arg1	complex					778:784	capsule-like complex	765:784	capsule-like complex	765:784	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	7	16	theme	capsule-like	1247:1258	arg1	complex					1260:1266	capsule-like complex	1247:1266	capsule-like complex	1247:1266	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	8	17	dep	Types	1443:1447	arg1	B.					1455:1456	B.	1455:1456	B.	1455:1456	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	17	dep	Types	1443:1447	arg1	A					1449:1449	A	1449:1449	A	1449:1449	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	17	dep	Types	1443:1447	arg1	Types					1443:1447	Types A and B.	1443:1456	F. tularensis Types A and B.	1429:1456	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	7	18	theme	complex	1260:1266	arg1	loss					1226:1229	loss	1226:1229	loss of O-antigen or capsule-like complex	1226:1266	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	6	19	theme	polysaccharide	1052:1065	arg1	composition					1067:1077	polysaccharide composition	1052:1077	polysaccharide composition of the biofilm matrix	1052:1099	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	4	20	dep	capable	684:690	arg1	strain					659:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	20	dep	capable	684:690	arg1	capable					684:690	capable	684:690	capable	684:690	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	20	dep	capable	684:690	arg1	%					677:677	50%	675:677	50%	675:677	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	7	21	theme	novicida	1300:1307	arg1	formation					1317:1325	F. novicida biofilm formation	1297:1325	F. novicida biofilm formation	1297:1325	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	3	22	theme	poor	447:450	arg1	biofilms					452:459	poor biofilms	447:459	poor biofilms	447:459	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	8	23	theme	environmental	1539:1551	arg1	niches					1553:1558	aquatic or other environmental niches	1522:1558	niches	1553:1558	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	3	24	dep	Types	428:432	arg1	Types					428:432	F. tularensis Types	414:432	F. tularensis Types A and B	414:440	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	24	dep	Types	428:432	arg1	B					440:440	B	440:440	B	440:440	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	24	dep	Types	428:432	arg1	A					434:434	A	434:434	A	434:434	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	25	theme	capsule-like	549:560	arg1	complex					562:568	capsule-like complex	549:568	capsule-like complex	549:568	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	5	26	theme	biofilm	869:875	arg1	formation					856:864	the formation	852:864	the formation	852:864	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	7	27	theme	B	1211:1211	arg1	strains					1213:1219	Type A or B strains	1201:1219	Type A or B strains	1201:1219	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	6	28	theme	matrix	1094:1099	arg1	composition					1067:1077	polysaccharide composition	1052:1077	polysaccharide composition of the biofilm matrix	1052:1099	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	4	29	from	O-antigen	751:759	arg1	deficient					738:746	deficient	738:746	deficient	738:746	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	3	30	theme	15-fold	583:589	arg1	biofilm					596:602	up to 15-fold more biofilm	577:602	up to 15-fold more biofilm	577:602	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	8	31	theme	aquatic	1522:1528	arg1	niches					1553:1558	aquatic or other environmental niches	1522:1558	niches	1553:1558	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	2	32	dep	pathogen	294:301	arg1	tularensis					315:324	Francisella tularensis	303:324	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages.
31439876	3	33	theme	F.	414:415	arg1	Types					428:432	F. tularensis Types	414:432	F. tularensis Types A and B	414:440	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	33	theme	F.	414:415	arg1	B					440:440	B	440:440	B	440:440	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	3	33	theme	F.	414:415	arg1	A					434:434	A	434:434	A	434:434	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	6	34	theme	polysaccharide	983:996	arg1	composition					998:1008	the surface polysaccharide composition	971:1008	the surface polysaccharide composition of the bacterium	971:1025	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	8	35	dep	niches	1553:1558	arg1	has					1560:1562	has	1560:1562	has yet to be determined	1560:1583	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	4	36	theme	deficient	738:746	arg1	mutants					730:736	mutants	730:736	mutants deficient in O-antigen and capsule-like complex	730:784	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	2	37	theme	mammalian	391:399	arg1	macrophages					401:411	mammalian macrophages	391:411	mammalian macrophages	391:411	The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages.
31439876	0	38	theme	Francisella	17:27	arg1	tularensis					29:38	Francisella tularensis	17:38	the Francisella tularensis Biofilm	13:46	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	3	39	dep	F.	466:467	arg1	tularensis					469:478	F. tularensis	466:478	F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex	466:568	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	4	40	from	deficient	738:746	arg1	O-antigen					751:759	O-antigen	751:759	O-antigen	751:759	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	40	from	deficient	738:746	arg1	complex					778:784	capsule-like complex	765:784	capsule-like complex	765:784	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	8	41	theme	polysaccharides	1380:1394	arg1	suppression					1357:1367	suppression	1357:1367	suppression of surface polysaccharides	1357:1394	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	3	42	theme	F.	466:467	arg1	mutants					480:486	F. tularensis mutants	466:486	F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex	466:568	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	1	43	theme	matrix-associated	151:167	arg1	Biofilms					138:145	Biofilms	138:145	Biofilms	138:145	Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria.
31439876	1	43	theme	matrix-associated	151:167	arg1	communities					169:179	matrix-associated communities	151:179	matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria	151:261	Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria.
31439876	4	44	theme	B	644:644	arg1	strain					659:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	44	theme	B	644:644	arg1	capable					684:690	capable	684:690	capable	684:690	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	44	theme	B	644:644	arg1	%					677:677	50%	675:677	50%	675:677	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	0	45	theme	Biofilm	40:46	arg1	Formation					0:8	Formation	0:8	Formation of the Francisella tularensis Biofilm	0:46	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	7	46	theme	robust	1181:1186	arg1	biofilm					1188:1194	a more robust biofilm	1174:1194	a more robust biofilm	1174:1194	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	7	47	theme	biofilm	1309:1315	arg1	formation					1317:1325	F. novicida biofilm formation	1297:1325	F. novicida biofilm formation	1297:1325	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	0	48	theme	tularensis	29:38	arg1	Biofilm					40:46	the Francisella tularensis Biofilm	13:46	the Francisella tularensis Biofilm	13:46	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	0	49	theme	Glucan	112:117	arg1	Exopolysaccharide					119:135	a Glucan Exopolysaccharide	110:135	a Glucan Exopolysaccharide	110:135	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	3	50	theme	glycosylated	615:626	arg1	cells					628:632	fully glycosylated cells	609:632	fully glycosylated cells	609:632	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	1	51	theme	unsuitable	227:236	arg1	environments					214:225	environments	214:225	environments unsuitable for free-living bacteria	214:261	Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria.
31439876	5	52	theme	growth	800:805	arg1	medium					807:812	the growth medium	796:812	the growth medium of all strains tested	796:834	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	7	53	theme	Type	1201:1204	arg1	A					1206:1206	Type A	1201:1206	Type A	1201:1206	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
31439876	8	54	from	survival	1493:1500	arg1	niches					1553:1558	aquatic or other environmental niches	1522:1558	niches	1553:1558	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	6	55	theme	surface	975:981	arg1	composition					998:1008	the surface polysaccharide composition	971:1008	the surface polysaccharide composition of the bacterium	971:1025	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	3	56	theme	lipopolysaccharide	496:513	arg1	O-antigen					515:523	lipopolysaccharide O-antigen	496:523	lipopolysaccharide O-antigen	496:523	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	5	57	theme	strains	821:827	arg1	medium					807:812	the growth medium	796:812	the growth medium of all strains tested	796:834	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	4	58	theme	surface	706:712	arg1	attachment					714:723	surface attachment	706:723	surface attachment than mutants deficient in O-antigen and capsule-like complex	706:784	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	8	59	theme	other	1533:1537	arg1	niches					1553:1558	aquatic or other environmental niches	1522:1558	niches	1553:1558	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	60	theme	biofilm	1466:1472	arg1	formation					1474:1482	biofilm formation	1466:1482	biofilm formation	1466:1482	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	61	dep	F.	1429:1430	arg1	B.					1455:1456	B.	1455:1456	B.	1455:1456	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	61	dep	F.	1429:1430	arg1	A					1449:1449	A	1449:1449	A	1449:1449	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	61	dep	F.	1429:1430	arg1	tularensis					1432:1441	F. tularensis Types A and B.	1429:1456	F. tularensis Types A and B.	1429:1456	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	8	61	dep	F.	1429:1430	arg1	Types					1443:1447	Types A and B.	1443:1456	F. tularensis Types A and B.	1429:1456	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	2	62	theme	Francisella	303:313	arg1	tularensis					315:324	Francisella tularensis	303:324	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages.
31439876	2	63	theme	intracellular	280:292	arg1	pathogen					294:301	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages.
31439876	5	64	theme	amylose-like	937:948	arg1	glucan					950:955	an amylose-like glucan	934:955	an amylose-like glucan	934:955	However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan.
31439876	1	65	theme	free-living	242:252	arg1	bacteria					254:261	free-living bacteria	242:261	free-living bacteria	242:261	Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria.
31439876	0	66	theme	Cell	63:66	arg1	Glycosylation					76:88	Cell Surface Glycosylation	63:88	Cell Surface Glycosylation	63:88	Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.
31439876	4	67	theme	vaccine	651:657	arg1	strain					659:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	67	theme	vaccine	651:657	arg1	capable					684:690	capable	684:690	capable	684:690	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	67	theme	vaccine	651:657	arg1	%					677:677	50%	675:677	50%	675:677	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	2	68	theme	facultative	268:278	arg1	pathogen					294:301	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis	264:324	The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages.
31439876	3	69	gly	glycosylated	615:626	arg1	cells					628:632	fully glycosylated cells	609:632	fully glycosylated cells	609:632	F. tularensis Types A and B form poor biofilms, but F. tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells.
31439876	8	70	theme	biofilm	1408:1414	arg1	formation					1416:1424	biofilm formation	1408:1424	biofilm formation	1408:1424	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	6	71	theme	biofilm	1086:1092	arg1	matrix					1094:1099	the biofilm matrix	1082:1099	the biofilm matrix	1082:1099	In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix.
31439876	4	72	theme	Type	639:642	arg1	strain					659:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	72	theme	Type	639:642	arg1	capable					684:690	capable	684:690	capable	684:690	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	72	theme	Type	639:642	arg1	%					677:677	50%	675:677	50%	675:677	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	8	73	theme	F.	1505:1506	arg1	survival					1493:1500	survival	1493:1500	survival of F. tularensis in aquatic or other environmental niches has yet to be determined	1493:1583	These results indicated that suppression of surface polysaccharides may promote biofilm formation by F. tularensis Types A and B. Whether biofilm formation enhances survival of F. tularensis in aquatic or other environmental niches has yet to be determined.
31439876	4	74	theme	live	646:649	arg1	strain					659:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain	635:664	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	74	theme	live	646:649	arg1	capable					684:690	capable	684:690	capable	684:690	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	4	74	theme	live	646:649	arg1	%					677:677	50%	675:677	50%	675:677	The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex.
31439876	7	75	theme	O-antigen	1234:1242	arg1	loss					1226:1229	loss	1226:1229	loss of O-antigen or capsule-like complex	1226:1266	In contrast, F. novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F. novicida biofilm formation.
30039738	1	0	from	content	196:202	arg1	protocol					264:271	an established lung decellularization protocol	226:271	an established lung decellularization protocol	226:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	0	1	theme	matrix	87:92	arg1	composition					94:104	extracellular matrix composition	73:104	extracellular matrix composition	73:104	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	7	2	located	observed	908:915	arg2	that					903:906	that	903:906	that	903:906	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	7	2	located	observed	908:915	arg1	lungs					936:940	ex vivo control lungs	920:940	ex vivo control lungs	920:940	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	7	3	dep	ex	920:921	arg1	vivo					923:926	vivo	923:926	vivo	923:926	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	1	4	theme	extracellular	175:187	arg1	content					196:202	extracellular matrix content	175:202	extracellular matrix content	175:202	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	2	5	used	used	312:315	arg2	mice					302:305	Healthy mice	294:305	Healthy mice	294:305	MATERIALS & METHODS Healthy mice were used: 15 for decellularization and five to serve as controls.
30039738	2	5	used	used	312:315	arg2	15					318:319	15	318:319	15	318:319	MATERIALS & METHODS Healthy mice were used: 15 for decellularization and five to serve as controls.
30039738	2	5	used	used	312:315	arg2	five					347:350	five	347:350	five	347:350	MATERIALS & METHODS Healthy mice were used: 15 for decellularization and five to serve as controls.
30039738	0	6	from	Impact	0:5	arg1	mechanics					110:118	mechanics	110:118	mechanics	110:118	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	0	6	from	Impact	0:5	arg1	composition					94:104	extracellular matrix composition	73:104	extracellular matrix composition	73:104	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	6	7	theme	dermatan	721:728	arg1	content					730:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content was reduced after decellularization.
30039738	1	8	from	rates	166:170	arg1	content					196:202	extracellular matrix content	175:202	extracellular matrix content	175:202	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	1	8	from	rates	166:170	arg1	mechanics					213:221	lung mechanics	208:221	lung mechanics	208:221	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	6	9	theme	heparan	709:715	arg1	content					730:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content was reduced after decellularization.
30039738	1	10	theme	matrix	189:194	arg1	content					196:202	extracellular matrix content	175:202	extracellular matrix content	175:202	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	3	11	theme	20 ml/min	409:417	arg1	rates					424:428	5, 10 and 20 ml/min flow rates	399:428	rates	424:428	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	3	12	theme	tracheal	438:445	arg1	cannula					447:453	tracheal cannula	438:453	tracheal cannula	438:453	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	7	13	theme	greater	867:873	arg1	preservation					875:886	greater preservation	867:886	greater preservation	867:886	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	7	14	theme	20 ml/min	816:824	arg1	rate					808:811	An intratracheal flow rate	786:811	An intratracheal flow rate of 20 ml/min	786:824	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	6	15	theme	Chondroitin	696:706	arg1	content					730:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content	696:736	Chondroitin, heparan and dermatan content was reduced after decellularization.
30039738	5	16	theme	Elastic	598:604	arg1	content					612:618	Elastic fiber content	598:618	Elastic fiber content	598:618	Elastic fiber content decreased at 5 and 10 ml/min, but not at 20 ml/min, compared with controls.
30039738	2	17	dep	METHODS	286:292	arg1	used					312:315	used	312:315	were used	307:315	MATERIALS & METHODS Healthy mice were used: 15 for decellularization and five to serve as controls.
30039738	0	18	theme	intratracheal	20:32	arg1	flows					34:38	different intratracheal flows	10:38	different intratracheal flows	10:38	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	4	19	theme	collagen	556:563	arg1	content					565:571	collagen content	556:571	collagen content in decellularized lungs	556:595	RESULTS The 20 ml/min rate better preserved collagen content in decellularized lungs.
30039738	5	20	theme	fiber	606:610	arg1	content					612:618	Elastic fiber content	598:618	Elastic fiber content	598:618	Elastic fiber content decreased at 5 and 10 ml/min, but not at 20 ml/min, compared with controls.
30039738	0	21	theme	different	10:18	arg1	flows					34:38	different intratracheal flows	10:38	different intratracheal flows	10:38	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	4	22	theme	decellularized	576:589	arg1	lungs					591:595	decellularized lungs	576:595	decellularized lungs	576:595	RESULTS The 20 ml/min rate better preserved collagen content in decellularized lungs.
30039738	7	23	theme	lower	846:850	arg1	resistance					852:861	lower resistance	846:861	lower resistance	846:861	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	3	24	theme	flow	419:422	arg1	rates					424:428	5, 10 and 20 ml/min flow rates	399:428	rates	424:428	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	7	25	theme	collagen	891:898	arg1	resistance					852:861	lower resistance	846:861	lower resistance	846:861	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	7	25	theme	collagen	891:898	arg1	preservation					875:886	greater preservation	867:886	greater preservation	867:886	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	1	26	theme	lung	208:211	arg1	mechanics					213:221	lung mechanics	208:221	lung mechanics	208:221	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	3	27	theme	right	459:463	arg1	0.5 ml/min					485:494	0.5 ml/min	485:494	0.5 ml/min	485:494	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	3	27	theme	right	459:463	arg1	cavity					477:482	right ventricular cavity	459:482	right ventricular cavity (0.5 ml/min)	459:495	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	0	28	theme	flows	34:38	arg1	Impact					0:5	Impact	0:5	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.	0:119	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	7	29	theme	flow	803:806	arg1	rate					808:811	An intratracheal flow rate	786:811	An intratracheal flow rate of 20 ml/min	786:824	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	4	30	from	content	565:571	arg1	lungs					591:595	decellularized lungs	576:595	decellularized lungs	576:595	RESULTS The 20 ml/min rate better preserved collagen content in decellularized lungs.
30039738	1	31	theme	established	229:239	arg1	protocol					264:271	an established lung decellularization protocol	226:271	an established lung decellularization protocol	226:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	3	32	theme	10 and	402:407	arg1	rates					424:428	5, 10 and 20 ml/min flow rates	399:428	rates	424:428	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	4	33	theme	20 ml/min	524:532	arg1	rate					534:537	The 20 ml/min rate	520:537	The 20 ml/min rate	520:537	RESULTS The 20 ml/min rate better preserved collagen content in decellularized lungs.
30039738	7	34	dep	CONCLUSION	775:784	arg1	associated					830:839	associated	830:839	was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs	826:940	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	0	35	theme	lung	47:50	arg1	decellularization					52:68	lung decellularization	47:68	lung decellularization	47:68	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	1	36	from	mechanics	213:221	arg1	protocol					264:271	an established lung decellularization protocol	226:271	an established lung decellularization protocol	226:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	7	37	theme	control	928:934	arg1	lungs					936:940	ex vivo control lungs	920:940	ex vivo control lungs	920:940	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	7	38	theme	intratracheal	789:801	arg1	rate					808:811	An intratracheal flow rate	786:811	An intratracheal flow rate of 20 ml/min	786:824	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	2	39	theme	Healthy	294:300	arg1	mice					302:305	Healthy mice	294:305	Healthy mice	294:305	MATERIALS & METHODS Healthy mice were used: 15 for decellularization and five to serve as controls.
30039738	1	40	theme	different	137:145	arg1	rates					166:170	different intratracheal flow rates	137:170	different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol	137:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	1	41	theme	lung	241:244	arg1	protocol					264:271	an established lung decellularization protocol	226:271	an established lung decellularization protocol	226:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	1	42	theme	intratracheal	147:159	arg1	rates					166:170	different intratracheal flow rates	137:170	different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol	137:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	7	43	theme	ex	920:921	arg1	lungs					936:940	ex vivo control lungs	920:940	ex vivo control lungs	920:940	CONCLUSION An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
30039738	1	44	theme	decellularization	246:262	arg1	protocol					264:271	an established lung decellularization protocol	226:271	an established lung decellularization protocol	226:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	0	45	theme	extracellular	73:85	arg1	composition					94:104	extracellular matrix composition	73:104	extracellular matrix composition	73:104	Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.
30039738	3	46	theme	ventricular	465:475	arg1	0.5 ml/min					485:494	0.5 ml/min	485:494	0.5 ml/min	485:494	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	3	46	theme	ventricular	465:475	arg1	cavity					477:482	right ventricular cavity	459:482	right ventricular cavity (0.5 ml/min)	459:495	Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
30039738	1	47	theme	flow	161:164	arg1	rates					166:170	different intratracheal flow rates	137:170	different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol	137:271	AIM To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
30039738	4	48	dep	preserved	546:554	arg1	content					565:571	collagen content	556:571	collagen content in decellularized lungs	556:595	RESULTS The 20 ml/min rate better preserved collagen content in decellularized lungs.
29883698	0	0	theme	silver	81:86	arg1	nanoparticles					88:100	in situ generated silver nanoparticles	63:100	in situ generated silver nanoparticles as fillers for antibacterial applications	63:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	1	1	theme	5 wt	300:303	arg1	%					304:304	1 wt% to 5 wt%	291:304	1 wt% to 5 wt%	291:304	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	6	2	theme	nanocomposites	1127:1140	arg1	strength					1104:1111	The tensile strength	1092:1111	The tensile strength of the hybrid nanocomposites	1092:1140	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	2	3	from	formation	367:375	arg1	surface					411:417	the surface	407:417	the surface of the NGFs	407:429	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	6	4	theme	tensile	1096:1102	arg1	strength					1104:1111	The tensile strength	1092:1111	The tensile strength of the hybrid nanocomposites	1092:1140	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	0	5	theme	generated	71:79	arg1	nanoparticles					88:100	in situ generated silver nanoparticles	63:100	in situ generated silver nanoparticles as fillers for antibacterial applications	63:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	7	6	theme	negative	1341:1348	arg1	bacteria					1390:1397	Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1336:1397	Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1336:1397	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	6	7	theme	hybrid	1120:1125	arg1	nanocomposites					1127:1140	the hybrid nanocomposites	1116:1140	the hybrid nanocomposites	1116:1140	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	2	8	theme	NGFs	426:429	arg1	surface					411:417	the surface	407:417	the surface of the NGFs	407:429	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	1	9	theme	nanocomposite	247:259	arg1	fillers					279:285	fillers	279:285	fillers (by 1 wt% to 5 wt%) in cellulose matrix	279:325	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	9	theme	nanocomposite	247:259	arg1	NGFs					261:264	these nanocomposite NGFs	241:264	these nanocomposite NGFs	241:264	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	10	theme	electron	458:465	arg1	SEM					479:481	SEM	479:481	SEM	479:481	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	10	theme	electron	458:465	arg1	microscope					467:476	scanning electron microscope	449:476	scanning electron microscope (SEM)	449:482	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	7	11	theme	good	1300:1303	arg1	activity					1319:1326	good antibacterial activity	1300:1326	good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1300:1397	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	2	12	theme	scanning	449:456	arg1	SEM					479:481	SEM	479:481	SEM	479:481	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	12	theme	scanning	449:456	arg1	microscope					467:476	scanning electron microscope	449:476	scanning electron microscope (SEM)	449:482	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	1	13	theme	cellulose	310:318	arg1	matrix					320:325	cellulose matrix	310:325	cellulose matrix	310:325	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	14	theme	X-ray	562:566	arg1	EDX					582:584	EDX	582:584	EDX	582:584	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	14	theme	X-ray	562:566	arg1	spectroscope					568:579	Energy dispersive X-ray spectroscope	544:579	Energy dispersive X-ray spectroscope (EDX)	544:585	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	7	15	theme	nanocomposite	1273:1285	arg1	films					1287:1291	The hybrid nanocomposite films	1262:1291	The hybrid nanocomposite films	1262:1291	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	1	16	used	used	271:274	arg2	NGFs					261:264	these nanocomposite NGFs	241:264	these nanocomposite NGFs	241:264	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	16	used	used	271:274	arg2	fillers					279:285	fillers	279:285	fillers (by 1 wt% to 5 wt%) in cellulose matrix	279:325	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	17	from	fillers	279:285	arg1	matrix					320:325	cellulose matrix	310:325	cellulose matrix	310:325	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	18	theme	generated	388:396	arg1	AgNPs					398:402	in situ generated AgNPs	380:402	in situ generated AgNPs	380:402	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	3	19	theme	AgNPs	687:691	arg1	presence					665:672	the presence	661:672	the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range	661:805	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	0	20	theme	hybrid	10:15	arg1	nanocomposites					17:30	Cellulose hybrid nanocomposites	0:30	Cellulose hybrid nanocomposites	0:30	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	3	21	theme	HR-TEM	635:640	arg1	analysis					642:649	The HR-TEM analysis	631:649	The HR-TEM analysis	631:649	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	2	22	theme	Energy	544:549	arg1	EDX					582:584	EDX	582:584	EDX	582:584	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	22	theme	Energy	544:549	arg1	spectroscope					568:579	Energy dispersive X-ray spectroscope	544:579	Energy dispersive X-ray spectroscope (EDX)	544:585	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	23	theme	photoelectron	597:609	arg1	XPS					625:627	XPS	625:627	XPS	625:627	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	23	theme	photoelectron	597:609	arg1	spectroscope					611:622	X-ray photoelectron spectroscope	591:622	X-ray photoelectron spectroscope (XPS)	591:628	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	0	24	theme	Cellulose	0:8	arg1	nanocomposites					17:30	Cellulose hybrid nanocomposites	0:30	Cellulose hybrid nanocomposites	0:30	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	1	25	dep	%	304:304	arg1	to					297:298	to	297:298	to	297:298	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	26	theme	X-ray	591:595	arg1	XPS					625:627	XPS	625:627	XPS	625:627	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	26	theme	X-ray	591:595	arg1	spectroscope					611:622	X-ray photoelectron spectroscope	591:622	X-ray photoelectron spectroscope (XPS)	591:628	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	7	27	theme	hybrid	1266:1271	arg1	films					1287:1291	The hybrid nanocomposite films	1262:1291	The hybrid nanocomposite films	1262:1291	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	7	28	theme	antibacterial	1305:1317	arg1	activity					1319:1326	good antibacterial activity	1300:1326	good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1300:1397	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	3	29	theme	spherical	677:685	arg1	AgNPs					687:691	spherical AgNPs	677:691	spherical AgNPs	677:691	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	6	30	theme	tensile	1187:1193	arg1	modulus					1195:1201	their tensile modulus	1181:1201	their tensile modulus	1181:1201	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	4	31	theme	composite	877:885	arg1	films					887:891	the hybrid composite films	866:891	the hybrid composite films	866:891	The POM images indicated the randomly oriented fillers in the hybrid composite films.
29883698	5	32	theme	catalytic	993:1001	arg1	activity					1003:1010	catalytic activity	993:1010	catalytic activity of the AgNPs	993:1023	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	0	33	theme	antibacterial	117:129	arg1	applications					131:142	antibacterial applications	117:142	antibacterial applications	117:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	0	34	theme	grass	45:49	arg1	fibers					51:56	Napier grass fibers	38:56	Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications	38:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	7	35	dep	negative	1341:1348	arg1	coli					1354:1357	E. coli	1351:1357	E. coli	1351:1357	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	4	36	theme	POM	812:814	arg1	images					816:821	The POM images	808:821	The POM images	808:821	The POM images indicated the randomly oriented fillers in the hybrid composite films.
29883698	1	37	theme	hybrid	335:340	arg1	films					356:360	hybrid nanocomposite films	335:360	hybrid nanocomposite films	335:360	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	38	theme	Napier	210:215	arg1	NGFs					231:234	NGFs	231:234	NGFs	231:234	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	38	theme	Napier	210:215	arg1	fibers					223:228	Napier grass fibers	210:228	Napier grass fibers (NGFs)	210:235	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	0	39	theme	Napier	38:43	arg1	fibers					51:56	Napier grass fibers	38:56	Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications	38:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	6	40	theme	various	1237:1243	arg1	contents					1252:1259	various filler contents	1237:1259	various filler contents	1237:1259	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	4	41	theme	hybrid	870:875	arg1	films					887:891	the hybrid composite films	866:891	the hybrid composite films	866:891	The POM images indicated the randomly oriented fillers in the hybrid composite films.
29883698	1	42	theme	nanocomposite	342:354	arg1	films					356:360	hybrid nanocomposite films	335:360	hybrid nanocomposite films	335:360	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	43	theme	grass	217:221	arg1	NGFs					231:234	NGFs	231:234	NGFs	231:234	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	43	theme	grass	217:221	arg1	fibers					223:228	Napier grass fibers	210:228	Napier grass fibers (NGFs)	210:235	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	5	44	theme	AgNPs	1019:1023	arg1	activity					1003:1010	catalytic activity	993:1010	catalytic activity of the AgNPs	993:1023	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	2	45	theme	electron	514:521	arg1	HR-TEM					535:540	HR-TEM	535:540	HR-TEM	535:540	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	45	theme	electron	514:521	arg1	microscope					523:532	high resolution transmission electron microscope	485:532	high resolution transmission electron microscope (HR-TEM)	485:541	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	5	46	theme	residual	1031:1038	arg1	higher					1060:1065	higher	1060:1065	higher	1060:1065	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	5	46	theme	residual	1031:1038	arg1	weight					1040:1045	the residual weight	1027:1045	the residual weight for them	1027:1054	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	5	47	theme	inflection	905:914	arg1	lower					959:963	lower	959:963	lower	959:963	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	5	47	theme	inflection	905:914	arg1	temperatures					916:927	the inflection temperatures	901:927	the inflection temperatures of the hybrid composites	901:952	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	3	48	attach	presence	665:672	arg2	AgNPs					687:691	spherical AgNPs	677:691	spherical AgNPs	677:691	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	3	48	attach	presence	665:672	arg1	surface					700:706	the surface	696:706	the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range	696:805	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	1	49	theme	1 wt	291:294	arg1	%					304:304	1 wt% to 5 wt%	291:304	1 wt% to 5 wt%	291:304	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	50	theme	transmission	501:512	arg1	HR-TEM					535:540	HR-TEM	535:540	HR-TEM	535:540	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	50	theme	transmission	501:512	arg1	microscope					523:532	high resolution transmission electron microscope	485:532	high resolution transmission electron microscope (HR-TEM)	485:541	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	7	51	dep	bacteria	1390:1397	arg1	aureus					1382:1387	S. aureus	1379:1387	S. aureus	1379:1387	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	2	52	theme	dispersive	551:560	arg1	EDX					582:584	EDX	582:584	EDX	582:584	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	52	theme	dispersive	551:560	arg1	spectroscope					568:579	Energy dispersive X-ray spectroscope	544:579	Energy dispersive X-ray spectroscope (EDX)	544:585	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	1	53	theme	%	295:295	arg1	%					304:304	1 wt% to 5 wt%	291:304	1 wt% to 5 wt%	291:304	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	2	54	theme	resolution	490:499	arg1	HR-TEM					535:540	HR-TEM	535:540	HR-TEM	535:540	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	54	theme	resolution	490:499	arg1	microscope					523:532	high resolution transmission electron microscope	485:532	high resolution transmission electron microscope (HR-TEM)	485:541	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	7	55	theme	positive	1369:1376	arg1	bacteria					1390:1397	Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1336:1397	Gram negative (E. coli) and Gram positive (S. aureus) bacteria	1336:1397	The hybrid nanocomposite films showed good antibacterial activity against Gram negative (E. coli) and Gram positive (S. aureus) bacteria.
29883698	3	56	theme	fillers	715:721	arg1	surface					700:706	the surface	696:706	the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range	696:805	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	2	57	theme	high	485:488	arg1	HR-TEM					535:540	HR-TEM	535:540	HR-TEM	535:540	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	2	57	theme	high	485:488	arg1	microscope					523:532	high resolution transmission electron microscope	485:532	high resolution transmission electron microscope (HR-TEM)	485:541	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	5	58	theme	hybrid	936:941	arg1	composites					943:952	the hybrid composites	932:952	the hybrid composites	932:952	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	3	59	dep	range	801:805	arg1	to					792:793	to	792:793	to	792:793	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	6	60	theme	filler	1245:1250	arg1	contents					1252:1259	various filler contents	1237:1259	various filler contents	1237:1259	The tensile strength of the hybrid nanocomposites varied between 73 MPa and 40 MPa while their tensile modulus between 4350 MPa and 2580 MPa for various filler contents.
29883698	5	61	theme	composites	943:952	arg1	lower					959:963	lower	959:963	lower	959:963	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	5	61	theme	composites	943:952	arg1	temperatures					916:927	the inflection temperatures	901:927	the inflection temperatures of the hybrid composites	901:952	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	0	62	with	fibers	51:56	arg1	nanoparticles					88:100	in situ generated silver nanoparticles	63:100	in situ generated silver nanoparticles as fillers for antibacterial applications	63:142	Cellulose hybrid nanocomposites using Napier grass fibers with in situ generated silver nanoparticles as fillers for antibacterial applications.
29883698	1	63	theme	silver	155:160	arg1	nanoparticles					162:174	Initially silver nanoparticles	145:174	Initially silver nanoparticles (AgNPs)	145:182	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	1	63	theme	silver	155:160	arg1	AgNPs					177:181	AgNPs	177:181	AgNPs	177:181	Initially silver nanoparticles (AgNPs) were in situ generated in Napier grass fibers (NGFs) and these nanocomposite NGFs were used as fillers (by 1 wt% to 5 wt%) in cellulose matrix to make hybrid nanocomposite films.
29883698	4	64	from	fillers	855:861	arg1	films					887:891	the hybrid composite films	866:891	the hybrid composite films	866:891	The POM images indicated the randomly oriented fillers in the hybrid composite films.
29883698	4	65	theme	oriented	846:853	arg1	fillers					855:861	the randomly oriented fillers	833:861	the randomly oriented fillers in the hybrid composite films	833:891	The POM images indicated the randomly oriented fillers in the hybrid composite films.
29883698	5	66	dep	lower	959:963	arg1	due					986:988	due	986:988	due	986:988	Though the inflection temperatures of the hybrid composites were lower than for the matrix (due to catalytic activity of the AgNPs), the residual weight for them was higher than that of the matrix.
29883698	3	67	with	fillers	715:721	arg1	size					730:733	a size	728:733	a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range	728:805	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	2	68	theme	AgNPs	398:402	arg1	formation					367:375	The formation	363:375	The formation of in situ generated AgNPs on the surface of the NGFs	363:429	The formation of in situ generated AgNPs on the surface of the NGFs was studied using scanning electron microscope (SEM), high resolution transmission electron microscope (HR-TEM), Energy dispersive X-ray spectroscope (EDX) and X-ray photoelectron spectroscope (XPS).
29883698	3	69	theme	them	777:780	arg1	majority					765:772	majority	765:772	majority of them	765:780	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
29883698	3	70	from	presence	665:672	arg1	surface					700:706	the surface	696:706	the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range	696:805	The HR-TEM analysis indicated the presence of spherical AgNPs on the surface of the fillers with a size ranging from 10 to 100 nm but majority of them in the 11 to 20 nm range.
31396677	5	0	theme	biomass	1407:1413	arg1	production					1415:1424	biomass production	1407:1424	biomass production	1407:1424	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	2	1	from	growth	466:471	arg1	wastewater					476:485	wastewater	476:485	wastewater	476:485	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	5	2	theme	dry	1530:1532	arg1	biomass					1534:1540	total dry biomass	1524:1540	total dry biomass	1524:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	7	3	theme	large-scale	1845:1855	arg1	cultivation					1863:1873	large-scale algal cultivation	1845:1873	large-scale algal cultivation in wastewater and microalgal-based wastewater treatments	1845:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	5	4	theme	alternating	1363:1373	arg1	condition					1396:1404	alternating high-low temperature condition	1363:1404	alternating high-low temperature condition	1363:1404	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	6	5	theme	great	1713:1717	arg1	potential					1719:1727	great potential	1713:1727	great potential to generate algal biomass and alleviate the wastewater nutrients	1713:1792	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	5	6	theme	temperature	1384:1394	arg1	condition					1396:1404	alternating high-low temperature condition	1363:1404	alternating high-low temperature condition	1363:1404	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	3	7	theme	wastewater	760:769	arg1	removal					780:786	the algal biomass and wastewater nutrient removal	738:786	removal	780:786	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	8	theme	g	1070:1070	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	8	theme	g	1070:1070	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	9	dep	TP	1186:1187	arg1	%					1194:1194	83.0%	1190:1194	83.0%	1190:1194	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	9	dep	TP	1186:1187	arg1	%					1201:1201	96.5%	1197:1201	96.5%	1197:1201	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	9	dep	TP	1186:1187	arg1	%					1208:1208	97.8%	1204:1208	97.8%	1204:1208	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	9	dep	TP	1186:1187	arg1	%					1219:1219	99.2%	1215:1219	99.2%	1215:1219	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	6	10	theme	high-low	1675:1682	arg1	condition					1696:1704	the alternating high-low temperature condition	1659:1704	the alternating high-low temperature condition	1659:1704	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	3	11	dep	temperature	826:836	arg1	1					819:819	1	819:819	1	819:819	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	12	theme	most	1051:1054	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	12	theme	most	1051:1054	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	1	13	theme	energy	236:241	arg1	crisis					243:248	energy crisis	236:248	energy crisis	236:248	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	4	14	theme	biomass	1090:1096	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	14	theme	biomass	1090:1096	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	3	15	dep	alternating	884:894	arg1	3					881:881	3	881:881	3	881:881	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	1	16	theme	Coupling	146:153	arg1	cultivation					161:171	Coupling algal cultivation	146:171	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden	146:280	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	2	17	theme	biomass	545:551	arg1	production					553:562	microalgal biomass production	534:562	microalgal biomass production	534:562	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	6	18	theme	algal	1741:1745	arg1	biomass					1747:1753	algal biomass	1741:1753	algal biomass	1741:1753	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	3	19	dep	temperature	905:915	arg1	°C					921:922	35 °C	918:922	35 °C in the day	918:933	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	5	20	theme	lipid	1281:1285	arg1	content					1287:1293	lipid content	1281:1293	lipid content	1281:1293	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	7	21	theme	microalgal-based	1893:1908	arg1	treatments					1921:1930	microalgal-based wastewater treatments	1893:1930	microalgal-based wastewater treatments	1893:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	3	22	dep	regimes	809:815	arg1	temperature					855:865	(2) high temperature	846:865	(2) high temperature (35 °C)	846:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	22	dep	regimes	809:815	arg1	temperature					826:836	(1) low temperature	818:836	(1) low temperature (4 °C)	818:843	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	22	dep	regimes	809:815	arg1	°C					841:842	4 °C	839:842	4 °C	839:842	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	1	23	theme	wastewater	178:187	arg1	treatment					189:197	wastewater treatment	178:197	wastewater treatment	178:197	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	2	24	theme	nutrient	579:586	arg1	removal					588:594	wastewater nutrient removal	568:594	wastewater nutrient removal	568:594	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	0	25	from	Effects	0:6	arg1	cultivation					40:50	cultivation	40:50	cultivation of microalgae in municipal wastewater	40:88	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	1	26	with	cultivation	161:171	arg1	treatment					189:197	wastewater treatment	178:197	wastewater treatment	178:197	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	3	27	dep	temperature	855:865	arg1	2					847:847	2	847:847	2	847:847	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	2	28	from	effects	496:502	arg1	removal					588:594	wastewater nutrient removal	568:594	wastewater nutrient removal	568:594	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	2	28	from	effects	496:502	arg1	production					553:562	microalgal biomass production	534:562	microalgal biomass production	534:562	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	5	29	theme	polysaccharides	1254:1268	arg1	analysis					1336:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	5	30	theme	lipid	1500:1504	arg1	content					1506:1512	the highest lipid content	1488:1512	the highest lipid content	1488:1512	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	5	31	theme	proteins	1271:1278	arg1	analysis					1336:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	4	32	theme	99.21	1115:1119	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	32	theme	99.21	1115:1119	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	2	33	theme	temperature	420:430	arg1	fluctuation					432:442	daily and seasonal temperature fluctuation	401:442	daily and seasonal temperature fluctuation	401:442	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	1	34	theme	increased	300:308	arg1	attention					310:318	the increased attention	296:318	the increased attention	296:318	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	4	35	theme	other	970:974	arg1	regimes					992:998	the other two temperature regimes	966:998	the other two temperature regimes	966:998	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	5	36	theme	composition	1324:1334	arg1	analysis					1336:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	4	37	theme	L-1	1124:1126	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	37	theme	L-1	1124:1126	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	0	38	theme	multi-temperature	11:27	arg1	regimes					29:35	multi-temperature regimes	11:35	multi-temperature regimes	11:35	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	3	39	from	°C	921:922	arg1	day					931:933	the day	927:933	the day	927:933	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	2	40	theme	temperature	511:521	arg1	regimes					523:529	the temperature regimes	507:529	the temperature regimes	507:529	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	2	41	theme	microalgal	455:464	arg1	growth					466:471	microalgal growth	455:471	microalgal growth in wastewater	455:485	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	5	42	theme	fatty	1300:1304	arg1	ester					1318:1322	fatty acid methyl ester	1300:1322	fatty acid methyl ester	1300:1322	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	3	43	theme	municipal	687:695	arg1	wastewater					697:706	municipal wastewater	687:706	municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C)	687:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	5	44	theme	biomass	1534:1540	arg1	%					1519:1519	26.4%	1515:1519	26.4% of total dry biomass	1515:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	5	44	theme	biomass	1534:1540	arg1	biomass					1534:1540	total dry biomass	1524:1540	total dry biomass	1524:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	7	45	from	cultivation	1863:1873	arg1	treatments					1921:1930	microalgal-based wastewater treatments	1893:1930	microalgal-based wastewater treatments	1893:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	7	45	from	cultivation	1863:1873	arg1	wastewater					1878:1887	wastewater	1878:1887	wastewater	1878:1887	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	4	46	theme	high-low	1005:1012	arg1	conditions					1026:1035	the high-low temperature conditions	1001:1035	the high-low temperature conditions	1001:1035	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	5	47	theme	methyl	1311:1316	arg1	ester					1318:1322	fatty acid methyl ester	1300:1322	fatty acid methyl ester	1300:1322	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	4	48	theme	efficient	1145:1153	arg1	removal					1155:1161	most efficient removal	1140:1161	most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively)	1140:1234	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	2	49	theme	microalgal-based	352:367	arg1	processes					369:377	these microalgal-based processes	346:377	these microalgal-based processes	346:377	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	3	50	from	night	944:948	arg1	°C					938:939	4 °C	936:939	4 °C at night	936:948	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	51	theme	temperature	980:990	arg1	regimes					992:998	the other two temperature regimes	966:998	the other two temperature regimes	966:998	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	3	52	from	effects	727:733	arg1	regimes					809:815	three temperature regimes	791:815	three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C)	791:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	52	from	effects	727:733	arg1	biomass					748:754	the algal biomass and wastewater nutrient removal	738:786	biomass	748:754	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	52	from	effects	727:733	arg1	removal					780:786	the algal biomass and wastewater nutrient removal	738:786	removal	780:786	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	53	theme	TP	1186:1187	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	53	theme	TP	1186:1187	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	53	theme	TP	1186:1187	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	53	theme	TP	1186:1187	arg1	removal					1155:1161	most efficient removal	1140:1161	most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively)	1140:1234	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	53	theme	TP	1186:1187	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	5	54	theme	total	1524:1528	arg1	biomass					1534:1540	total dry biomass	1524:1540	total dry biomass	1524:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	3	55	theme	algal	742:746	arg1	biomass					748:754	the algal biomass and wastewater nutrient removal	738:786	biomass	748:754	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	7	56	theme	algal	1857:1861	arg1	cultivation					1863:1873	large-scale algal cultivation	1845:1873	large-scale algal cultivation in wastewater and microalgal-based wastewater treatments	1845:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	4	57	theme	1.62	1065:1068	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	57	theme	1.62	1065:1068	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	5	58	theme	high-low	1375:1382	arg1	condition					1396:1404	alternating high-low temperature condition	1363:1404	alternating high-low temperature condition	1363:1404	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	3	59	dep	°C	921:922	arg1	°C					938:939	4 °C	936:939	4 °C at night	936:948	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	5	60	theme	biofuel	1454:1460	arg1	production					1462:1471	biofuel production	1454:1471	biofuel production	1454:1471	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	3	61	theme	nutrient	771:778	arg1	removal					780:786	the algal biomass and wastewater nutrient removal	738:786	removal	780:786	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	7	62	theme	valuable	1820:1827	arg1	information					1829:1839	some valuable information	1815:1839	some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments	1815:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	6	63	theme	temperature	1684:1694	arg1	condition					1696:1704	the alternating high-low temperature condition	1659:1704	the alternating high-low temperature condition	1659:1704	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	6	64	theme	alternating	1663:1673	arg1	condition					1696:1704	the alternating high-low temperature condition	1659:1704	the alternating high-low temperature condition	1659:1704	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	3	65	theme	temperature	797:807	arg1	regimes					809:815	three temperature regimes	791:815	three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C)	791:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	66	theme	production	1098:1107	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	66	theme	production	1098:1107	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	3	67	theme	low	822:824	arg1	temperature					855:865	(2) high temperature	846:865	(2) high temperature (35 °C)	846:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	67	theme	low	822:824	arg1	temperature					826:836	(1) low temperature	818:836	(1) low temperature (4 °C)	818:843	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	67	theme	low	822:824	arg1	°C					841:842	4 °C	839:842	4 °C	839:842	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	0	68	theme	municipal	69:77	arg1	wastewater					79:88	municipal wastewater	69:88	municipal wastewater	69:88	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	1	69	theme	algal	155:159	arg1	cultivation					161:171	Coupling algal cultivation	146:171	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden	146:280	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	3	70	theme	high	850:853	arg1	temperature					855:865	(2) high temperature	846:865	(2) high temperature (35 °C)	846:873	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	70	theme	high	850:853	arg1	°C					871:872	35 °C	868:872	35 °C	868:872	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	3	70	theme	high	850:853	arg1	temperature					826:836	(1) low temperature	818:836	(1) low temperature (4 °C)	818:843	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	2	71	theme	microalgal	534:543	arg1	production					553:562	microalgal biomass production	534:562	microalgal biomass production	534:562	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	5	72	dep	was	1484:1486	arg1	%					1519:1519	26.4%	1515:1519	26.4% of total dry biomass	1515:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	5	72	dep	was	1484:1486	arg1	biomass					1534:1540	total dry biomass	1524:1540	total dry biomass	1524:1540	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	4	73	theme	highest	1082:1088	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	73	theme	highest	1082:1088	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	2	74	theme	regimes	523:529	arg1	effects					496:502	the effects	492:502	the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal	492:594	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	5	75	theme	content	1287:1293	arg1	analysis					1336:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	7	76	theme	wastewater	1910:1919	arg1	treatments					1921:1930	microalgal-based wastewater treatments	1893:1930	microalgal-based wastewater treatments	1893:1930	This study provides some valuable information for large-scale algal cultivation in wastewater and microalgal-based wastewater treatments.
31396677	2	77	theme	wastewater	568:577	arg1	removal					588:594	wastewater nutrient removal	568:594	wastewater nutrient removal	568:594	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	0	78	from	cultivation	40:50	arg1	wastewater					79:88	municipal wastewater	69:88	municipal wastewater	69:88	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	4	79	theme	mg	1121:1122	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	79	theme	mg	1121:1122	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	2	80	theme	seasonal	411:418	arg1	fluctuation					432:442	daily and seasonal temperature fluctuation	401:442	daily and seasonal temperature fluctuation	401:442	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	0	81	theme	regimes	29:35	arg1	Effects					0:6	Effects	0:6	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater	0:88	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	4	82	theme	TN	1171:1172	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	82	theme	TN	1171:1172	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	82	theme	TN	1171:1172	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	82	theme	TN	1171:1172	arg1	removal					1155:1161	most efficient removal	1140:1161	most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively)	1140:1234	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	82	theme	TN	1171:1172	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	6	83	theme	wastewater	1773:1782	arg1	nutrients					1784:1792	the wastewater nutrients	1769:1792	the wastewater nutrients	1769:1792	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	1	84	theme	recent	323:328	arg1	years					330:334	recent years	323:334	recent years	323:334	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
31396677	3	85	theme	high-low	896:903	arg1	temperature					905:915	high-low temperature	896:915	high-low temperature (35 °C in the day: 4 °C at night)	896:949	In this study, Chlorella vulgaris was continuously cultured for 15 days in municipal wastewater to investigate the effects on the algal biomass and wastewater nutrient removal in three temperature regimes: (1) low temperature (4 °C), (2) high temperature (35 °C), and (3) alternating high-low temperature (35 °C in the day: 4 °C at night).
31396677	4	86	theme	NH3-N	1175:1179	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	86	theme	NH3-N	1175:1179	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	86	theme	NH3-N	1175:1179	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	86	theme	NH3-N	1175:1179	arg1	removal					1155:1161	most efficient removal	1140:1161	most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively)	1140:1234	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	86	theme	NH3-N	1175:1179	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	5	87	theme	highest	1492:1498	arg1	content					1506:1512	the highest lipid content	1488:1512	the highest lipid content	1488:1512	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	0	88	theme	microalgae	55:64	arg1	cultivation					40:50	cultivation	40:50	cultivation of microalgae in municipal wastewater	40:88	Effects of multi-temperature regimes on cultivation of microalgae in municipal wastewater to simultaneously remove nutrients and produce biomass.
31396677	5	89	theme	acid	1306:1309	arg1	ester					1318:1322	fatty acid methyl ester	1300:1322	fatty acid methyl ester	1300:1322	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	5	90	theme	ester	1318:1322	arg1	analysis					1336:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis	1250:1343	In addition, the polysaccharides, proteins, lipid content, and fatty acid methyl ester composition analysis indicates that in alternating high-low temperature condition, biomass production increased the potential for biofuel production, and there was the highest lipid content (26.4% of total dry biomass).
31396677	4	91	theme	COD	1166:1168	arg1	day-1					1128:1132	99.21 mg L-1 day-1	1115:1132	99.21 mg L-1 day-1	1115:1132	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	91	theme	COD	1166:1168	arg1	biomass					1056:1062	the most biomass	1047:1062	the most biomass (1.62 g L-1)	1047:1075	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	91	theme	COD	1166:1168	arg1	rate					1109:1112	the highest biomass production rate	1078:1112	the highest biomass production rate (99.21 mg L-1 day-1)	1078:1133	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	91	theme	COD	1166:1168	arg1	removal					1155:1161	most efficient removal	1140:1161	most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively)	1140:1234	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	91	theme	COD	1166:1168	arg1	L-1					1072:1074	1.62 g L-1	1065:1074	1.62 g L-1	1065:1074	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	4	92	theme	temperature	1014:1024	arg1	conditions					1026:1035	the high-low temperature conditions	1001:1035	the high-low temperature conditions	1001:1035	Compared with the other two temperature regimes, the high-low temperature conditions generated the most biomass (1.62 g L-1), the highest biomass production rate (99.21 mg L-1 day-1), and most efficient removal of COD, TN, NH3-N, and TP (83.0%, 96.5%, 97.8%, and 99.2%, respectively).
31396677	6	93	theme	temperature	1631:1641	arg1	conditions					1643:1652	these temperature conditions	1625:1652	these temperature conditions	1625:1652	The results showed that the nutrients except COD were all efficiently removed in these temperature conditions, and the alternating high-low temperature condition showed great potential to generate algal biomass and alleviate the wastewater nutrients.
31396677	2	94	theme	daily	401:405	arg1	fluctuation					432:442	daily and seasonal temperature fluctuation	401:442	daily and seasonal temperature fluctuation	401:442	However, these microalgal-based processes are challenging since daily and seasonal temperature fluctuation may affect microalgal growth in wastewater, and the effects of the temperature regimes on microalgal biomass production and wastewater nutrient removal remain unclear.
31396677	1	95	theme	environmental	261:273	arg1	burden					275:280	environmental burden	261:280	environmental burden	261:280	Coupling algal cultivation with wastewater treatment due to their potentials to alleviate energy crisis and reduce environmental burden has attracted the increased attention in recent years.
30772509	2	0	theme	InP/ZnS	600:606	arg1	dots					616:619	fluorescent InP/ZnS quantum dots	588:619	fluorescent InP/ZnS quantum dots (QDs)	588:625	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	0	theme	InP/ZnS	600:606	arg1	QDs					622:624	QDs	622:624	QDs	622:624	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	4	1	with	coating	868:874	arg1	shell					890:894	a silica shell	881:894	a silica shell	881:894	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	4	2	theme	tight	933:937	arg1	shell					954:958	a tight polysaccharide shell	931:958	(ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method	926:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	1	3	theme	easy	290:293	arg1	detection					295:303	an easy detection	287:303	an easy detection in vivo	287:311	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	9	4	dep	in	2062:2063	arg1	vivo					2065:2068	vivo	2065:2068	vivo	2065:2068	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	4	5	theme	particles	1003:1011	arg1	surface					977:983	the surface	973:983	the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method	973:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	9	6	theme	luminescent	2032:2042	arg1	degree					2127:2132	a versatile degree	2115:2132	a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2115:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	6	theme	luminescent	2032:2042	arg1	fluorescent					1969:1979	fluorescent	1969:1979	fluorescent	1969:1979	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	6	theme	luminescent	2032:2042	arg1	STMS					1992:1995	hybrid STMS	1985:1995	hybrid STMS	1985:1995	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	6	theme	luminescent	2032:2042	arg1	nanoplatforms					2044:2056	novel luminescent nanoplatforms	2026:2056	novel luminescent nanoplatforms for in vivo fluorescence tracking applications	2026:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	1	7	theme	good	208:211	arg1	stability					223:231	good colloidal stability	208:231	good colloidal stability in aqueous solutions	208:252	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	1	7	theme	good	208:211	arg1	scaling-up					196:205	scaling-up	196:205	scaling-up	196:205	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	8	8	theme	microscopy	1769:1778	arg1	imaging					1780:1786	in vivo confocal microscopy imaging	1752:1786	in vivo confocal microscopy imaging	1752:1786	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	6	9	theme	metal	1408:1412	arg1	release					1421:1427	metal cation release	1408:1427	metal cation release	1408:1427	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	1	10	theme	aqueous	236:242	arg1	solutions					244:252	aqueous solutions	236:252	aqueous solutions	236:252	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	4	11	theme	further	860:866	arg1	coating					868:874	a further coating	858:874	a further coating with a silica shell	858:894	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	4	12	theme	@	998:998	arg1	particles					1003:1011	these STMS@QDs particles	988:1011	these STMS@QDs particles	988:1011	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	9	13	theme	tracking	2083:2090	arg1	applications					2092:2103	in vivo fluorescence tracking applications	2062:2103	in vivo fluorescence tracking applications	2062:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	8	14	theme	remanent	1910:1917	arg1	labelling					1931:1939	a remanent fluorescent labelling	1908:1939	a remanent fluorescent labelling of ZF tissues	1908:1953	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	7	15	theme	luminescent	1500:1510	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	5	16	from	monodispersity	1187:1200	arg1	solutions					1271:1279	aqueous solutions	1263:1279	aqueous solutions	1263:1279	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	4	17	theme	-mediated	1049:1057	arg1	method					1059:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	7	18	theme	ZF	1631:1632	arg1	embryos					1647:1653	zebrafish (ZF) translucent embryos	1620:1653	zebrafish (ZF) translucent embryos	1620:1653	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	7	19	theme	@	1484:1484	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	2	20	theme	denoted	565:571	arg1	morphology					553:562	a stellate morphology	542:562	a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	542:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	20	theme	denoted	565:571	arg1	STMS					573:576	denoted STMS	565:576	denoted STMS	565:576	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	7	21	theme	zebrafish	1620:1628	arg1	embryos					1647:1653	zebrafish (ZF) translucent embryos	1620:1653	zebrafish (ZF) translucent embryos	1620:1653	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	7	22	theme	@	1480:1480	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	4	23	theme	isobutyramide	1029:1041	arg1	method					1059:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	5	24	theme	size	1182:1185	arg1	monodispersity					1187:1200	a high size monodispersity	1175:1200	a high size monodispersity (ca. 200 and 100 nm respectively)	1175:1234	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	24	theme	size	1182:1185	arg1	structure					1164:1172	a highly controlled structure	1144:1172	a highly controlled structure	1144:1172	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	7	25	theme	original	1467:1474	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	4	26	theme	IBAM	1044:1047	arg1	method					1059:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	9	27	theme	additional	2137:2146	arg1	functionalities					2148:2162	additional functionalities	2137:2162	additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2137:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	7	28	dep	shown	1530:1534	arg1	biocompatible					1536:1548	biocompatible	1536:1548	biocompatible	1536:1548	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	8	29	dep	in	1752:1753	arg1	vivo					1755:1758	vivo	1755:1758	vivo	1755:1758	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	9	30	dep	fluorescent	1969:1979	arg1	composites					1997:2006	composites	1997:2006	composites	1997:2006	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	4	31	theme	silica	883:888	arg1	shell					890:894	a silica shell	881:894	a silica shell	881:894	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	1	32	dep	nanoplatforms	131:143	arg1	providing					277:285	providing	277:285	providing an easy detection in vivo	277:311	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	1	32	dep	nanoplatforms	131:143	arg1	displaying					173:182	displaying	173:182	displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility,	173:271	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	8	33	theme	neighboring	1878:1888	arg1	tissues					1890:1896	neighboring tissues	1878:1896	neighboring tissues	1878:1896	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	7	34	theme	cancer	1571:1576	arg1	cells					1578:1582	murine cancer cells	1564:1582	murine cancer cells	1564:1582	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	8	35	theme	tissues	1947:1953	arg1	labelling					1931:1939	a remanent fluorescent labelling	1908:1939	a remanent fluorescent labelling of ZF tissues	1908:1953	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	2	36	theme	high	657:660	arg1	%					672:672	≥90%	669:672	≥90%	669:672	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	36	theme	high	657:660	arg1	yield					662:666	a high yield	655:666	a high yield (≥90%)	655:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	37	theme	original	417:424	arg1	strategy					426:433	The original strategy	413:433	The original strategy proposed here	413:447	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	0	38	theme	Wrapped	0:6	arg1	silica					17:22	Wrapped stellate silica	0:22	Wrapped stellate silica	0:22	Wrapped stellate silica nanocomposites as biocompatible luminescent nanoplatforms assessed in vivo.
30772509	2	39	theme	large	469:473	arg1	pore					475:478	large pore	469:478	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	39	theme	large	469:473	arg1	15 nm					485:489	ca. 15 nm	481:489	ca. 15 nm	481:489	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	9	40	theme	magnetic/plasmonic	2197:2214	arg1	core					2216:2219	magnetic/plasmonic core	2197:2219	magnetic/plasmonic core	2197:2219	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	6	41	theme	IBAM-polysaccharide	1306:1324	arg1	approach					1334:1341	the IBAM-polysaccharide coating approach	1302:1341	the IBAM-polysaccharide coating approach	1302:1341	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	1	42	theme	fluorescence	316:327	arg1	methods					329:335	fluorescence methods	316:335	fluorescence methods	316:335	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	1	43	theme	luminescent	119:129	arg1	nanoplatforms					131:143	luminescent nanoplatforms	119:143	luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo	119:311	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	2	44	theme	mesoporous	492:501	arg1	NPs					530:532	NPs	530:532	NPs	530:532	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	44	theme	mesoporous	492:501	arg1	nanoparticles					515:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles	469:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	8	45	theme	fluorescent	1919:1929	arg1	labelling					1931:1939	a remanent fluorescent labelling	1908:1939	a remanent fluorescent labelling of ZF tissues	1908:1953	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	0	46	theme	luminescent	56:66	arg1	nanoplatforms					68:80	biocompatible luminescent nanoplatforms	42:80	biocompatible luminescent nanoplatforms assessed in vivo	42:97	Wrapped stellate silica nanocomposites as biocompatible luminescent nanoplatforms assessed in vivo.
30772509	1	47	theme	high	352:355	arg1	potential					357:365	high potential	352:365	high potential of functionalities	352:384	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	6	48	dep	suitable	1361:1368	arg1	avoid					1402:1406	avoid	1402:1406	to avoid metal cation release over three months	1399:1445	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	6	48	dep	suitable	1361:1368	arg1	ensure					1373:1378	ensure	1373:1378	to ensure QDs protection	1370:1393	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	5	49	theme	novel	1101:1105	arg1	nanoplatforms					1119:1131	novel luminescent nanoplatforms	1101:1131	novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions	1101:1279	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	9	50	theme	hybrid	1985:1990	arg1	degree					2127:2132	a versatile degree	2115:2132	a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2115:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	50	theme	hybrid	1985:1990	arg1	fluorescent					1969:1979	fluorescent	1969:1979	fluorescent	1969:1979	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	50	theme	hybrid	1985:1990	arg1	STMS					1992:1995	hybrid STMS	1985:1995	hybrid STMS	1985:1995	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	50	theme	hybrid	1985:1990	arg1	nanoplatforms					2044:2056	novel luminescent nanoplatforms	2026:2056	novel luminescent nanoplatforms for in vivo fluorescence tracking applications	2026:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	5	51	theme	colloidal	1240:1248	arg1	stability					1250:1258	colloidal stability	1240:1258	colloidal stability	1240:1258	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	51	theme	colloidal	1240:1248	arg1	structure					1164:1172	a highly controlled structure	1144:1172	a highly controlled structure	1144:1172	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	52	dep	monodispersity	1187:1200	arg1	100 nm					1215:1220	100 nm	1215:1220	100 nm	1215:1220	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	52	dep	monodispersity	1187:1200	arg1	200					1207:1209	200	1207:1209	200	1207:1209	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	4	53	theme	polysaccharide	939:952	arg1	shell					954:958	a tight polysaccharide shell	931:958	(ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method	926:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	5	54	theme	wrapping	1072:1079	arg1	approaches					1081:1090	Both wrapping approaches	1067:1090	Both wrapping approaches	1067:1090	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	2	55	theme	fluorescent	588:598	arg1	dots					616:619	fluorescent InP/ZnS quantum dots	588:619	fluorescent InP/ZnS quantum dots (QDs)	588:625	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	55	theme	fluorescent	588:598	arg1	QDs					622:624	QDs	622:624	QDs	622:624	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	7	56	theme	toxicity	1672:1679	arg1	sign					1664:1667	no sign	1661:1667	no sign of toxicity	1661:1679	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	8	57	theme	in	1752:1753	arg1	imaging					1780:1786	in vivo confocal microscopy imaging	1752:1786	in vivo confocal microscopy imaging	1752:1786	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	4	58	theme	wrapping	822:829	arg1	methods					831:837	two wrapping methods	818:837	two wrapping methods	818:837	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	9	59	theme	novel	2026:2030	arg1	degree					2127:2132	a versatile degree	2115:2132	a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2115:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	59	theme	novel	2026:2030	arg1	fluorescent					1969:1979	fluorescent	1969:1979	fluorescent	1969:1979	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	59	theme	novel	2026:2030	arg1	STMS					1992:1995	hybrid STMS	1985:1995	hybrid STMS	1985:1995	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	59	theme	novel	2026:2030	arg1	nanoplatforms					2044:2056	novel luminescent nanoplatforms	2026:2056	novel luminescent nanoplatforms for in vivo fluorescence tracking applications	2026:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	8	60	theme	confocal	1760:1767	arg1	microscopy					1769:1778	confocal microscopy	1760:1778	in vivo confocal microscopy imaging	1752:1786	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	4	61	theme	potential	784:792	arg1	release					794:800	potential release	784:800	potential release	784:800	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	4	62	theme	original	1020:1027	arg1	method					1059:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	an original isobutyramide (IBAM)-mediated method	1017:1064	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	4	63	theme	small	903:907	arg1	≤2 nm					916:920	≤2 nm	916:920	≤2 nm	916:920	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	4	63	theme	small	903:907	arg1	pores					909:913	small pores	903:913	small pores (≤2 nm)	903:921	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	6	64	theme	cation	1414:1419	arg1	release					1421:1427	metal cation release	1408:1427	metal cation release	1408:1427	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	1	65	theme	colloidal	213:221	arg1	stability					223:231	good colloidal stability	208:231	good colloidal stability in aqueous solutions	208:252	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	1	65	theme	colloidal	213:221	arg1	scaling-up					196:205	scaling-up	196:205	scaling-up	196:205	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	2	66	theme	quantum	608:614	arg1	dots					616:619	fluorescent InP/ZnS quantum dots	588:619	fluorescent InP/ZnS quantum dots (QDs)	588:625	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	66	theme	quantum	608:614	arg1	QDs					622:624	QDs	622:624	QDs	622:624	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	7	67	theme	translucent	1635:1645	arg1	embryos					1647:1653	zebrafish (ZF) translucent embryos	1620:1653	zebrafish (ZF) translucent embryos	1620:1653	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	4	68	theme	STMS	994:997	arg1	particles					1003:1011	these STMS@QDs particles	988:1011	these STMS@QDs particles	988:1011	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	9	69	theme	in	2062:2063	arg1	applications					2092:2103	in vivo fluorescence tracking applications	2062:2103	in vivo fluorescence tracking applications	2062:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	2	70	theme	stellate	544:551	arg1	morphology					553:562	a stellate morphology	542:562	a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	542:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	70	theme	stellate	544:551	arg1	STMS					573:576	denoted STMS	565:576	denoted STMS	565:576	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	9	71	theme	fluorescence	2070:2081	arg1	applications					2092:2103	in vivo fluorescence tracking applications	2062:2103	in vivo fluorescence tracking applications	2062:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	7	72	theme	polysaccharide	1485:1498	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	3	73	theme	potential	737:745	arg1	release					751:757	a potential QDs release	735:757	a potential QDs release	735:757	These nanoplatforms are after that further coated to avoid a potential QDs release.
30772509	1	74	theme	nanoplatforms	131:143	arg1	engineering					104:114	The engineering	100:114	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods	100:335	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	1	74	theme	nanoplatforms	131:143	arg1	challenge					402:410	a challenge	400:410	a challenge	400:410	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	4	75	dep	having	896:901	arg1	i					855:855	i	855:855	i	855:855	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	1	76	theme	biomedical	149:158	arg1	applications					160:171	biomedical applications	149:171	biomedical applications	149:171	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	7	77	theme	STMS	1476:1479	arg1	nanoplatforms					1512:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	these original STMS@QDs@polysaccharide luminescent nanoplatforms	1461:1524	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	5	78	theme	high	1177:1180	arg1	monodispersity					1187:1200	a high size monodispersity	1175:1200	a high size monodispersity (ca. 200 and 100 nm respectively)	1175:1234	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	78	theme	high	1177:1180	arg1	structure					1164:1172	a highly controlled structure	1144:1172	a highly controlled structure	1144:1172	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	9	79	theme	versatile	2117:2125	arg1	degree					2127:2132	a versatile degree	2115:2132	a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2115:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	79	theme	versatile	2117:2125	arg1	fluorescent					1969:1979	fluorescent	1969:1979	fluorescent	1969:1979	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	79	theme	versatile	2117:2125	arg1	STMS					1992:1995	hybrid STMS	1985:1995	hybrid STMS	1985:1995	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	79	theme	versatile	2117:2125	arg1	nanoplatforms					2044:2056	novel luminescent nanoplatforms	2026:2056	novel luminescent nanoplatforms for in vivo fluorescence tracking applications	2026:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	8	80	theme	blood	1847:1851	arg1	circulation					1853:1863	blood circulation	1847:1863	blood circulation	1847:1863	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	1	81	from	stability	223:231	arg1	solutions					244:252	aqueous solutions	236:252	aqueous solutions	236:252	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	2	82	theme	MS	511:512	arg1	NPs					530:532	NPs	530:532	NPs	530:532	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	82	theme	MS	511:512	arg1	nanoparticles					515:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles	469:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	1	83	theme	functionalities	370:384	arg1	potential					357:365	high potential	352:365	high potential of functionalities	352:384	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	2	84	theme	silica	503:508	arg1	NPs					530:532	NPs	530:532	NPs	530:532	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	84	theme	silica	503:508	arg1	nanoparticles					515:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles	469:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	5	85	theme	controlled	1153:1162	arg1	stability					1250:1258	colloidal stability	1240:1258	colloidal stability	1240:1258	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	85	theme	controlled	1153:1162	arg1	monodispersity					1187:1200	a high size monodispersity	1175:1200	a high size monodispersity (ca. 200 and 100 nm respectively)	1175:1234	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	5	85	theme	controlled	1153:1162	arg1	structure					1164:1172	a highly controlled structure	1144:1172	a highly controlled structure	1144:1172	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	9	86	theme	functionalities	2148:2162	arg1	degree					2127:2132	a versatile degree	2115:2132	a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core)	2115:2220	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	86	theme	functionalities	2148:2162	arg1	fluorescent					1969:1979	fluorescent	1969:1979	fluorescent	1969:1979	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	86	theme	functionalities	2148:2162	arg1	STMS					1992:1995	hybrid STMS	1985:1995	hybrid STMS	1985:1995	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	86	theme	functionalities	2148:2162	arg1	nanoplatforms					2044:2056	novel luminescent nanoplatforms	2026:2056	novel luminescent nanoplatforms for in vivo fluorescence tracking applications	2026:2103	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	4	87	dep	shell	954:958	arg1	ii					927:928	ii	927:928	ii	927:928	To protect the QDs from potential release or dissolution, two wrapping methods are developed: (i) a further coating with a silica shell having small pores (≤2 nm) or (ii) a tight polysaccharide shell deposited on the surface of these STMS@QDs particles via an original isobutyramide (IBAM)-mediated method.
30772509	2	88	contain	having	535:540	arg1	NPs					530:532	NPs	530:532	NPs	530:532	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	88	contain	having	535:540	arg2	STMS					573:576	denoted STMS	565:576	denoted STMS	565:576	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	88	contain	having	535:540	arg2	morphology					553:562	a stellate morphology	542:562	a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	542:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	88	contain	having	535:540	arg1	nanoparticles					515:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles	469:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	9	89	theme	drug	2165:2168	arg1	incorporation					2180:2192	incorporation	2180:2192	incorporation of magnetic/plasmonic core	2180:2219	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	89	theme	drug	2165:2168	arg1	delivery					2170:2177	drug delivery	2165:2177	drug delivery	2165:2177	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	5	90	from	stability	1250:1258	arg1	solutions					1271:1279	aqueous solutions	1263:1279	aqueous solutions	1263:1279	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	2	91	theme	nanoparticles	515:527	arg1	use					462:464	the use	458:464	the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	458:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	0	92	theme	stellate	8:15	arg1	silica					17:22	Wrapped stellate silica	0:22	Wrapped stellate silica	0:22	Wrapped stellate silica nanocomposites as biocompatible luminescent nanoplatforms assessed in vivo.
30772509	2	93	theme	pore	475:478	arg1	NPs					530:532	NPs	530:532	NPs	530:532	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	93	theme	pore	475:478	arg1	nanoparticles					515:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles	469:527	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	6	94	theme	coating	1326:1332	arg1	approach					1334:1341	the IBAM-polysaccharide coating approach	1302:1341	the IBAM-polysaccharide coating approach	1302:1341	Among both methods, the IBAM-polysaccharide coating approach is shown the most suitable to ensure QDs protection and to avoid metal cation release over three months.
30772509	7	95	with	biocompatible	1536:1548	arg1	cells					1578:1582	murine cancer cells	1564:1582	murine cancer cells	1564:1582	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	7	96	theme	murine	1564:1569	arg1	cells					1578:1582	murine cancer cells	1564:1582	murine cancer cells	1564:1582	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	9	97	theme	core	2216:2219	arg1	incorporation					2180:2192	incorporation	2180:2192	incorporation of magnetic/plasmonic core	2180:2219	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	97	theme	core	2216:2219	arg1	delivery					2170:2177	drug delivery	2165:2177	drug delivery	2165:2177	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	0	98	theme	biocompatible	42:54	arg1	nanoplatforms					68:80	biocompatible luminescent nanoplatforms	42:80	biocompatible luminescent nanoplatforms assessed in vivo	42:97	Wrapped stellate silica nanocomposites as biocompatible luminescent nanoplatforms assessed in vivo.
30772509	1	99	dep	challenge	402:410	arg1	offering					343:350	offering	343:350	offering high potential of functionalities	343:384	The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge.
30772509	5	100	theme	aqueous	1263:1269	arg1	solutions					1271:1279	aqueous solutions	1263:1279	aqueous solutions	1263:1279	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	8	101	theme	ZF	1944:1945	arg1	tissues					1947:1953	ZF tissues	1944:1953	ZF tissues	1944:1953	As assessed by in vivo confocal microscopy imaging, these nanoplatforms are shown to rapidly extravasate from blood circulation to settle in neighboring tissues, ensuring a remanent fluorescent labelling of ZF tissues in vivo.
30772509	2	102	theme	ca.	481:483	arg1	pore					475:478	large pore	469:478	large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)	469:673	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	2	102	theme	ca.	481:483	arg1	15 nm					485:489	ca. 15 nm	481:489	ca. 15 nm	481:489	The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%).
30772509	7	103	theme	several	1723:1729	arg1	days					1731:1734	several days	1723:1734	several days	1723:1734	Furthermore, these original STMS@QDs@polysaccharide luminescent nanoplatforms are shown biocompatible in vitro with murine cancer cells and in vivo after injections within zebrafish (ZF) translucent embryos where no sign of toxicity is observed during their development over several days.
30772509	5	104	theme	luminescent	1107:1117	arg1	nanoplatforms					1119:1131	novel luminescent nanoplatforms	1101:1131	novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions	1101:1279	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30772509	9	105	dep	functionalities	2148:2162	arg1	incorporation					2180:2192	incorporation	2180:2192	incorporation of magnetic/plasmonic core	2180:2219	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	9	105	dep	functionalities	2148:2162	arg1	delivery					2170:2177	drug delivery	2165:2177	drug delivery	2165:2177	Such fluorescent and hybrid STMS composites are envisioned as novel luminescent nanoplatforms for in vivo fluorescence tracking applications and offer a versatile degree of additional functionalities (drug delivery, incorporation of magnetic/plasmonic core).
30772509	5	106	dep	200	1207:1209	arg1	ca.					1203:1205	ca.	1203:1205	ca.	1203:1205	Both wrapping approaches yield to novel luminescent nanoplatforms displaying a highly controlled structure, a high size monodispersity (ca. 200 and 100 nm respectively) and colloidal stability in aqueous solutions.
30862945	3	0	theme	accessory	318:326	arg1	enzymes					328:334	key and accessory enzymes	310:334	key and accessory enzymes responsible for CO2 sequestration	310:368	Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration.
30862945	4	1	theme	polysaccharide	512:525	arg1	B					527:527	polysaccharide B	512:527	polysaccharide B	512:527	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	4	1	theme	polysaccharide	512:525	arg1	lipopolysaccharide					472:489	lipopolysaccharide	472:489	lipopolysaccharide	472:489	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	11	2	theme	%	1249:1249	arg1	activity					1270:1277	68% ± 0.9 flocculating activity	1247:1277	68% ± 0.9 flocculating activity	1247:1277	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	8	3	theme	linkage	975:981	arg1	groups					946:951	The functional groups	931:951	The functional groups	931:951	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	3	theme	linkage	975:981	arg1	EPS					1018:1020	purified EPS	1009:1020	purified EPS	1009:1020	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	3	theme	linkage	975:981	arg1	analysis					983:990	linkage analysis	975:990	linkage analysis	975:990	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	1	4	theme	carbon	157:162	arg1	CO2					173:175	CO2	173:175	CO2	173:175	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	1	4	theme	carbon	157:162	arg1	dioxide					164:170	a previously reported carbon dioxide	135:170	a previously reported carbon dioxide (CO2)	135:176	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	6	5	theme	process	775:781	arg1	parameters					783:792	process parameters	775:792	process parameters	775:792	After optimizing process parameters, the EPS production increased more than 3 folds.
30862945	10	6	theme	kaolin	1202:1207	arg1	bioflocculation					1185:1199	bioflocculation	1185:1199	bioflocculation (kaolin test)	1185:1213	EPS was further applied for bioflocculation (kaolin test) and dye removal.
30862945	10	6	theme	kaolin	1202:1207	arg1	test					1209:1212	kaolin test	1202:1212	kaolin test	1202:1212	EPS was further applied for bioflocculation (kaolin test) and dye removal.
30862945	8	7	dep	1H	1059:1060	arg1	NMR					1070:1072	NMR	1070:1072	NMR	1070:1072	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	4	8	theme	capsular	530:537	arg1	polysaccharide					539:552	capsular polysaccharide	530:552	capsular polysaccharide	530:552	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	4	8	theme	capsular	530:537	arg1	lipopolysaccharide					472:489	lipopolysaccharide	472:489	lipopolysaccharide	472:489	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	12	9	theme	sequestration	1382:1394	arg1	potential					1415:1423	CO2 sequestration and EPS production potential	1378:1423	CO2 sequestration and EPS production potential of Serratia sp	1378:1438	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	1	10	theme	dioxide	164:170	arg1	analysis					123:130	genomic analysis	115:130	genomic analysis of a previously reported carbon dioxide (CO2)	115:176	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	5	11	theme	EPS	610:612	arg1	0.88 ± 0.08					630:640	0.88 ± 0.08	630:640	0.88 ± 0.08	630:640	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	11	theme	EPS	610:612	arg1	production					596:605	The production	592:605	The production of EPS	592:612	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	7	12	theme	EPS	897:899	arg1	strain					861:866	strain	861:866	strain	861:866	The morphology of strain and elemental composition of EPS was characterized by SEM-EDX.
30862945	7	12	theme	EPS	897:899	arg1	composition					882:892	elemental composition	872:892	elemental composition	872:892	The morphology of strain and elemental composition of EPS was characterized by SEM-EDX.
30862945	11	13	theme	68	1247:1248	arg1	%					1249:1249	%	1249:1249	%	1249:1249	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	8	14	theme	purified	1009:1016	arg1	EPS					1018:1020	purified EPS	1009:1020	purified EPS	1009:1020	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	7	15	theme	elemental	872:880	arg1	composition					882:892	elemental composition	872:892	elemental composition	872:892	The morphology of strain and elemental composition of EPS was characterized by SEM-EDX.
30862945	3	16	theme	CO2	352:354	arg1	sequestration					356:368	CO2 sequestration	352:368	CO2 sequestration	352:368	Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration.
30862945	8	17	theme	EPS	1018:1020	arg1	composition					962:972	monomer composition	954:972	monomer composition	954:972	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	17	theme	EPS	1018:1020	arg1	structure					996:1004	structure	996:1004	structure	996:1004	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	17	theme	EPS	1018:1020	arg1	groups					946:951	The functional groups	931:951	The functional groups	931:951	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	17	theme	EPS	1018:1020	arg1	EPS					1018:1020	purified EPS	1009:1020	purified EPS	1009:1020	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	17	theme	EPS	1018:1020	arg1	analysis					983:990	linkage analysis	975:990	linkage analysis	975:990	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	7	18	theme	composition	882:892	arg1	morphology					847:856	The morphology	843:856	The morphology of strain and elemental composition of EPS	843:899	The morphology of strain and elemental composition of EPS was characterized by SEM-EDX.
30862945	12	19	theme	CO2	1378:1380	arg1	sequestration					1382:1394	CO2 sequestration	1378:1394	CO2 sequestration	1378:1394	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	7	20	theme	strain	861:866	arg1	morphology					847:856	The morphology	843:856	The morphology of strain and elemental composition of EPS	843:899	The morphology of strain and elemental composition of EPS was characterized by SEM-EDX.
30862945	11	21	theme	acridine	1308:1315	arg1	orange					1317:1322	cationic dye acridine orange	1295:1322	cationic dye acridine orange (80%)	1295:1328	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	21	theme	acridine	1308:1315	arg1	%					1327:1327	80%	1325:1327	80%	1325:1327	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	22	theme	crystal	1334:1340	arg1	violet					1342:1347	crystal violet	1334:1347	crystal violet (95%)	1334:1353	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	22	theme	crystal	1334:1340	arg1	%					1352:1352	95%	1350:1352	95%	1350:1352	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	23	theme	flocculating	1257:1268	arg1	activity					1270:1277	68% ± 0.9 flocculating activity	1247:1277	68% ± 0.9 flocculating activity	1247:1277	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	4	24	theme	synthesis	375:383	arg1	genes					385:389	EPS synthesis genes	371:389	EPS synthesis genes	371:389	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	0	25	theme	Genomic	0:6	arg1	analysis					8:15	Genomic analysis	0:15	Genomic analysis of carbon dioxide	0:33	Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.
30862945	5	26	from	1.44 ± 0.10 g L-1	659:675	arg1	NaHCO3					702:707	NaHCO3	702:707	NaHCO3	702:707	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	26	from	1.44 ± 0.10 g L-1	659:675	arg1	NaHCO3					714:719	NaHCO3	714:719	NaHCO3	714:719	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	26	from	1.44 ± 0.10 g L-1	659:675	arg1	bicarbonate					689:699	bicarbonate	689:699	bicarbonate (NaHCO3)	689:708	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	26	from	1.44 ± 0.10 g L-1	659:675	arg1	glucose					680:686	glucose	680:686	glucose	680:686	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	12	27	theme	Serratia	1428:1435	arg1	sp					1437:1438	Serratia sp	1428:1438	Serratia sp	1428:1438	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	6	28	theme	EPS	799:801	arg1	production					803:812	the EPS production	795:812	the EPS production	795:812	After optimizing process parameters, the EPS production increased more than 3 folds.
30862945	1	29	theme	present	100:106	arg1	study					108:112	the present study	96:112	the present study	96:112	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	0	30	theme	carbon	20:25	arg1	dioxide					27:33	carbon dioxide	20:33	carbon dioxide	20:33	Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.
30862945	1	31	theme	bacterium	191:199	arg1	sp					210:211	bacterium Serratia sp	191:211	bacterium Serratia sp	191:211	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	11	32	theme	dye	1304:1306	arg1	orange					1317:1322	cationic dye acridine orange	1295:1322	cationic dye acridine orange (80%)	1295:1328	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	32	theme	dye	1304:1306	arg1	%					1327:1327	80%	1325:1327	80%	1325:1327	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	5	33	from	1.25 ± 0.13	643:653	arg1	NaHCO3					702:707	NaHCO3	702:707	NaHCO3	702:707	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	33	from	1.25 ± 0.13	643:653	arg1	NaHCO3					714:719	NaHCO3	714:719	NaHCO3	714:719	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	33	from	1.25 ± 0.13	643:653	arg1	bicarbonate					689:699	bicarbonate	689:699	bicarbonate (NaHCO3)	689:708	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	33	from	1.25 ± 0.13	643:653	arg1	glucose					680:686	glucose	680:686	glucose	680:686	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	3	34	theme	Genomic	282:288	arg1	analysis					290:297	Genomic analysis	282:297	Genomic analysis	282:297	Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration.
30862945	1	35	theme	Serratia	201:208	arg1	sp					210:211	bacterium Serratia sp	191:211	bacterium Serratia sp	191:211	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	4	36	theme	responsible	456:466	arg1	clusters					447:454	8 putative clusters	436:454	8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production	436:589	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	6	37	dep	3	834:834	arg1	folds					836:840	folds	836:840	folds	836:840	After optimizing process parameters, the EPS production increased more than 3 folds.
30862945	0	38	theme	dioxide	27:33	arg1	analysis					8:15	Genomic analysis	0:15	Genomic analysis of carbon dioxide	0:33	Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.
30862945	12	39	theme	production	1404:1413	arg1	potential					1415:1423	CO2 sequestration and EPS production potential	1378:1423	CO2 sequestration and EPS production potential of Serratia sp	1378:1438	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	5	40	from	pH	750:751	arg1	1.44 ± 0.10 g L-1					659:675	1.44 ± 0.10 g L-1	659:675	1.44 ± 0.10 g L-1	659:675	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	40	from	pH	750:751	arg1	1.25 ± 0.13					643:653	1.25 ± 0.13	643:653	1.25 ± 0.13	643:653	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	40	from	pH	750:751	arg1	0.88 ± 0.08					630:640	0.88 ± 0.08	630:640	0.88 ± 0.08	630:640	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	40	from	pH	750:751	arg1	production					596:605	The production	592:605	The production of EPS	592:612	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	11	41	theme	cationic	1295:1302	arg1	orange					1317:1322	cationic dye acridine orange	1295:1322	cationic dye acridine orange (80%)	1295:1328	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	11	41	theme	cationic	1295:1302	arg1	%					1327:1327	80%	1325:1327	80%	1325:1327	The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%).
30862945	3	42	theme	key	310:312	arg1	enzymes					328:334	key and accessory enzymes	310:334	key and accessory enzymes responsible for CO2 sequestration	310:368	Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration.
30862945	8	43	theme	functional	935:944	arg1	composition					962:972	monomer composition	954:972	monomer composition	954:972	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	43	theme	functional	935:944	arg1	structure					996:1004	structure	996:1004	structure	996:1004	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	43	theme	functional	935:944	arg1	groups					946:951	The functional groups	931:951	The functional groups	931:951	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	43	theme	functional	935:944	arg1	EPS					1018:1020	purified EPS	1009:1020	purified EPS	1009:1020	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	43	theme	functional	935:944	arg1	analysis					983:990	linkage analysis	975:990	linkage analysis	975:990	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	1	44	theme	genomic	115:121	arg1	analysis					123:130	genomic analysis	115:130	genomic analysis of a previously reported carbon dioxide (CO2)	115:176	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	12	45	theme	EPS	1400:1402	arg1	production					1404:1413	EPS production	1400:1413	EPS production	1400:1413	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	4	46	theme	acid-saccharide	564:578	arg1	lipopolysaccharide					472:489	lipopolysaccharide	472:489	lipopolysaccharide	472:489	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	4	46	theme	acid-saccharide	564:578	arg1	production					580:589	fatty acid-saccharide production	558:589	fatty acid-saccharide production	558:589	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	8	47	theme	monomer	954:960	arg1	groups					946:951	The functional groups	931:951	The functional groups	931:951	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	47	theme	monomer	954:960	arg1	composition					962:972	monomer composition	954:972	monomer composition	954:972	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	8	47	theme	monomer	954:960	arg1	EPS					1018:1020	purified EPS	1009:1020	purified EPS	1009:1020	The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR.
30862945	4	48	theme	EPS	371:373	arg1	genes					385:389	EPS synthesis genes	371:389	EPS synthesis genes	371:389	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	10	49	theme	dye	1219:1221	arg1	removal					1223:1229	dye removal	1219:1229	dye removal	1219:1229	EPS was further applied for bioflocculation (kaolin test) and dye removal.
30862945	4	50	theme	fatty	558:562	arg1	lipopolysaccharide					472:489	lipopolysaccharide	472:489	lipopolysaccharide	472:489	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	4	50	theme	fatty	558:562	arg1	production					580:589	fatty acid-saccharide production	558:589	fatty acid-saccharide production	558:589	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	9	51	located	detected	1136:1143	arg2	galactose					1084:1092	galactose	1084:1092	galactose	1084:1092	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	9	51	located	detected	1136:1143	arg2	mannose					1095:1101	mannose	1095:1101	mannose	1095:1101	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	9	51	located	detected	1136:1143	arg1	EPS					1152:1154	the EPS	1148:1154	the EPS	1148:1154	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	9	51	located	detected	1136:1143	arg2	monomers					1127:1134	the monomers	1123:1134	the monomers detected in the EPS	1123:1154	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	9	51	located	detected	1136:1143	arg2	glucosamine					1107:1117	glucosamine	1107:1117	glucosamine	1107:1117	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	9	51	located	detected	1136:1143	arg2	Glucose					1075:1081	Glucose	1075:1081	Glucose	1075:1081	Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS.
30862945	12	52	theme	sp	1437:1438	arg1	potential					1415:1423	CO2 sequestration and EPS production potential	1378:1423	CO2 sequestration and EPS production potential of Serratia sp	1378:1438	The results highlight CO2 sequestration and EPS production potential of Serratia sp.
30862945	3	53	theme	responsible	336:346	arg1	enzymes					328:334	key and accessory enzymes	310:334	key and accessory enzymes responsible for CO2 sequestration	310:368	Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration.
30862945	4	54	theme	putative	438:445	arg1	clusters					447:454	8 putative clusters	436:454	8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production	436:589	EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production.
30862945	2	55	theme	exopolysaccharide	246:262	arg1	production					270:279	exopolysaccharide (EPS) production	246:279	exopolysaccharide (EPS) production	246:279	ISTD04 was performed along with exopolysaccharide (EPS) production.
30862945	5	56	from	0.88 ± 0.08	630:640	arg1	NaHCO3					702:707	NaHCO3	702:707	NaHCO3	702:707	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	56	from	0.88 ± 0.08	630:640	arg1	NaHCO3					714:719	NaHCO3	714:719	NaHCO3	714:719	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	56	from	0.88 ± 0.08	630:640	arg1	bicarbonate					689:699	bicarbonate	689:699	bicarbonate (NaHCO3)	689:708	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	5	56	from	0.88 ± 0.08	630:640	arg1	glucose					680:686	glucose	680:686	glucose	680:686	The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L-1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8.
30862945	0	57	theme	exopolysaccharides	62:79	arg1	production					81:90	exopolysaccharides production	62:90	exopolysaccharides production	62:90	Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.
30862945	1	58	theme	reported	148:155	arg1	CO2					173:175	CO2	173:175	CO2	173:175	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30862945	1	58	theme	reported	148:155	arg1	dioxide					164:170	a previously reported carbon dioxide	135:170	a previously reported carbon dioxide (CO2)	135:176	In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp.
30248125	11	0	from	expression	1738:1747	arg1	plants					1760:1765	mAb COK plants	1752:1765	mAb COK plants	1752:1765	Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses.
30248125	6	1	theme	anti-colorectal	1084:1098	arg1	mAbs					1107:1110	anti-colorectal cancer mAbs	1084:1110	anti-colorectal cancer mAbs	1084:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	6	2	used	used	970:973	arg2	transformation					951:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	10	3	theme	COK	1644:1646	arg1	plants					1648:1653	COK plants	1644:1653	COK plants	1644:1653	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	8	4	dep	in	1457:1458	arg1	vitro					1460:1464	vitro	1460:1464	vitro	1460:1464	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	9	5	theme	CO	1586:1587	arg1	plants					1589:1594	CO plants	1586:1594	CO plants	1586:1594	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	15	6	theme	retention	2394:2402	arg1	peptide					2411:2417	ER retention signal peptide	2391:2417	ER retention signal peptide	2391:2417	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	16	7	theme	retention	2608:2616	arg1	peptide					2625:2631	ER retention signal peptide	2605:2631	ER retention signal peptide	2605:2631	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	1	8	theme	secretion	238:246	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	8	theme	secretion	238:246	arg1	reticulum					171:179	The endoplasmic reticulum	155:179	The endoplasmic reticulum (ER)	155:184	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	8	theme	secretion	238:246	arg1	secretion					238:246	secretion	238:246	secretion	238:246	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	8	theme	secretion	238:246	arg1	folding					225:231	folding	225:231	folding	225:231	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	8	theme	secretion	238:246	arg1	site					198:201	the main site	189:201	the main site of protein synthesis, folding, and secretion to other organelles	189:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	12	9	theme	extracellular	1920:1932	arg1	areas					1934:1938	extracellular areas	1920:1938	extracellular areas	1920:1938	mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas.
30248125	6	10	theme	tagging	1167:1173	arg1	effects					1151:1157	the physiological effects	1133:1157	the physiological effects of KDEL tagging	1133:1173	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	7	11	theme	mAb	1300:1302	arg1	CO					1304:1305	mAb CO	1300:1305	mAb CO	1300:1305	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	12	theme	Germination	1176:1186	arg1	rates					1188:1192	Germination rates	1176:1192	Germination rates	1176:1192	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	15	13	theme	culture	2547:2553	arg1	media					2555:2559	culture media	2547:2559	culture media	2547:2559	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	5	14	theme	chain	824:828	arg1	C-terminus					800:809	the C-terminus	796:809	the C-terminus of the heavy chain	796:828	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	12	15	dep	distributed	1826:1836	arg1	whereas					1879:1885	whereas	1879:1885	whereas	1879:1885	mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas.
30248125	14	16	theme	specific	2297:2304	arg1	residues					2313:2320	the plant specific glycan residues	2287:2320	the plant specific glycan residues	2287:2320	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	10	17	theme	mAb	1704:1706	arg1	amounts					1708:1714	up to five times higher expression and mAb amounts	1665:1714	amounts	1708:1714	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	6	18	theme	dip	947:949	arg1	transformation					951:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	6	19	theme	physiological	1137:1149	arg1	effects					1151:1157	the physiological effects	1133:1157	the physiological effects of KDEL tagging	1133:1173	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	3	20	theme	anti-cancer	514:524	arg1	mAbs					549:552	mAbs	549:552	mAbs	549:552	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	3	20	theme	anti-cancer	514:524	arg1	antibodies					537:546	anti-cancer monoclonal antibodies	514:546	anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2	514:605	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	12	21	theme	leaf	1865:1868	arg1	tissues					1870:1876	leaf tissues	1865:1876	leaf tissues	1865:1876	mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas.
30248125	6	22	theme	Agrobacterium-mediated	917:938	arg1	transformation					951:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	16	23	theme	signal	2618:2623	arg1	peptide					2625:2631	ER retention signal peptide	2605:2631	ER retention signal peptide	2605:2631	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	4	24	theme	antibody	619:626	arg1	CO17-1A					634:640	Monoclonal antibody (mAb) CO17-1A	608:640	Monoclonal antibody (mAb) CO17-1A	608:640	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	11	25	theme	mAb	1752:1754	arg1	plants					1760:1765	mAb COK plants	1752:1765	mAb COK plants	1752:1765	Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses.
30248125	0	26	theme	plant	132:136	arg1	response					145:152	plant stress response	132:152	plant stress response	132:152	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	13	27	theme	binding	2049:2055	arg1	activities					2057:2066	equivalent or slightly better binding activities	2019:2066	equivalent or slightly better binding activities	2019:2066	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	16	28	theme	proteins	2698:2705	arg1	yields					2676:2681	the yields	2672:2681	the yields of recombinant proteins	2672:2705	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	16	29	theme	recombinant	2686:2696	arg1	proteins					2698:2705	recombinant proteins	2686:2705	recombinant proteins	2686:2705	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	2	30	dep	capacity	273:280	arg1	process					295:301	process	295:301	to process proteins	292:310	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	9	31	with	genes	1496:1500	arg1	exception					1512:1520	the exception	1508:1520	the exception of bZIP28 and IRE1a	1508:1540	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	7	32	with	CO	1304:1305	arg1	COK					1321:1323	KDEL mAb COK	1312:1323	KDEL mAb COK (COK plant)	1312:1335	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	32	with	CO	1304:1305	arg1	plant					1330:1334	COK plant	1326:1334	COK plant	1326:1334	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	15	33	theme	signal	2404:2409	arg1	peptide					2411:2417	ER retention signal peptide	2391:2417	ER retention signal peptide	2391:2417	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	16	34	theme	ER	2605:2606	arg1	retention					2608:2616	ER retention	2605:2616	ER retention signal peptide	2605:2631	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	4	35	theme	colorectal	696:705	arg1	surface					719:725	the colorectal cancer cell surface	692:725	the colorectal cancer cell surface	692:725	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	13	36	theme	equivalent	2019:2028	arg1	activities					2057:2066	equivalent or slightly better binding activities	2019:2066	equivalent or slightly better binding activities	2019:2066	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	3	37	theme	antigen	591:597	arg1	GA733-2					599:605	the tumor-associated antigen GA733-2	570:605	the tumor-associated antigen GA733-2	570:605	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	15	38	theme	ER	2391:2392	arg1	peptide					2411:2417	ER retention signal peptide	2391:2417	ER retention signal peptide	2391:2417	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	8	39	theme	non-transgenic	1420:1433	arg1	plants					1447:1452	non-transgenic Arabidopsis plants	1420:1452	non-transgenic Arabidopsis plants	1420:1452	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	7	40	theme	mAb	1317:1319	arg1	COK					1321:1323	KDEL mAb COK	1312:1323	KDEL mAb COK (COK plant)	1312:1335	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	40	theme	mAb	1317:1319	arg1	plant					1330:1334	COK plant	1326:1334	COK plant	1326:1334	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	10	41	dep	five	1671:1674	arg1	to					1668:1669	to	1668:1669	to	1668:1669	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	14	42	theme	N-glycan	2260:2267	arg1	structure					2269:2277	an oligo-mannose N-glycan structure	2243:2277	an oligo-mannose N-glycan structure	2243:2277	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	5	43	theme	ER	732:733	arg1	sequence					774:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	13	44	theme	mAb	1990:1992	arg1	CO					1994:1995	mAb CO and mAb COK	1990:2007	CO	1994:1995	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	5	45	theme	cancer	856:861	arg1	mAbs					863:866	anti-colorectal cancer mAbs	840:866	anti-colorectal cancer mAbs	840:866	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	6	46	theme	T1	987:988	arg1	transformants					990:1002	T1 transformants	987:1002	T1 transformants	987:1002	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	5	47	theme	Lys-Asp-Glu-Leu	745:759	arg1	sequence					774:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	15	48	theme	plant	2355:2359	arg1	growth					2361:2366	plant growth	2355:2366	plant growth	2355:2366	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	1	49	theme	endoplasmic	159:169	arg1	ER					182:183	ER	182:183	ER	182:183	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	49	theme	endoplasmic	159:169	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	49	theme	endoplasmic	159:169	arg1	folding					225:231	folding	225:231	folding	225:231	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	49	theme	endoplasmic	159:169	arg1	site					198:201	the main site	189:201	the main site of protein synthesis, folding, and secretion to other organelles	189:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	49	theme	endoplasmic	159:169	arg1	reticulum					171:179	The endoplasmic reticulum	155:179	The endoplasmic reticulum (ER)	155:184	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	49	theme	endoplasmic	159:169	arg1	secretion					238:246	secretion	238:246	secretion	238:246	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	8	50	theme	in	1457:1458	arg1	media					1466:1470	in vitro media	1457:1470	in vitro media	1457:1470	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	13	51	theme	resonance	1957:1965	arg1	analyses					1967:1974	Surface plasmon resonance analyses	1941:1974	Surface plasmon resonance analyses	1941:1974	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	16	52	theme	effective	2639:2647	arg1	approach					2649:2656	an effective approach	2636:2656	an effective approach for enhancing the yields of recombinant proteins in vivo	2636:2713	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	16	52	theme	effective	2639:2647	arg1	fusion					2595:2600	the fusion	2591:2600	the fusion of ER retention signal peptide	2591:2631	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	14	53	theme	mAb	2221:2223	arg1	COK					2225:2227	mAb COK	2221:2227	mAb COK	2221:2227	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	2	54	theme	unfolded	354:361	arg1	proteins					377:384	unfolded and misfolded proteins	354:384	unfolded and misfolded proteins	354:384	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	15	55	theme	in	2436:2437	arg1	conditions					2445:2454	in vitro conditions	2436:2454	in vitro conditions	2436:2454	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	7	56	theme	KDEL	1312:1315	arg1	COK					1321:1323	KDEL mAb COK	1312:1323	KDEL mAb COK (COK plant)	1312:1335	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	56	theme	KDEL	1312:1315	arg1	plant					1330:1334	COK plant	1326:1334	COK plant	1326:1334	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	4	57	theme	cell	714:717	arg1	surface					719:725	the colorectal cancer cell surface	692:725	the colorectal cancer cell surface	692:725	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	0	58	theme	Endoplasmic	0:10	arg1	reticulum					12:20	Endoplasmic reticulum	0:20	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	0:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	5	59	theme	mAb	901:903	arg1	production					905:914	mAb production	901:914	mAb production	901:914	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	2	60	theme	ER	397:398	arg1	stress					400:405	ER stress	397:405	ER stress	397:405	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	14	61	theme	plant	2189:2193	arg1	residues					2204:2211	plant specific residues	2189:2211	plant specific residues	2189:2211	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	0	62	theme	retention	22:30	arg1	motif					32:36	Endoplasmic reticulum retention motif	0:36	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	0:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	9	63	theme	bZIP28	1525:1530	arg1	exception					1512:1520	the exception	1508:1520	the exception of bZIP28 and IRE1a	1508:1540	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	13	64	contain	possessed	2009:2017	arg2	activities					2057:2066	equivalent or slightly better binding activities	2019:2066	equivalent or slightly better binding activities	2019:2066	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	13	64	contain	possessed	2009:2017	arg1	CO					1994:1995	mAb CO and mAb COK	1990:2007	CO	1994:1995	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	13	64	contain	possessed	2009:2017	arg1	COK					2005:2007	mAb CO and mAb COK	1990:2007	COK	2005:2007	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	15	65	from	conditions	2445:2454	arg1	study					2331:2335	this study	2326:2335	this study	2326:2335	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	10	66	theme	Western	1597:1603	arg1	blot					1605:1608	Western blot	1597:1608	Western blot	1597:1608	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	8	67	theme	COK	1347:1349	arg1	lengths					1371:1377	COK plants primary root lengths	1347:1377	COK plants primary root lengths	1347:1377	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	2	68	theme	misfolded	367:375	arg1	proteins					377:384	unfolded and misfolded proteins	354:384	unfolded and misfolded proteins	354:384	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	5	69	theme	KDEL	762:765	arg1	sequence					774:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	13	70	theme	parental	2122:2129	arg1	antibody					2131:2138	a commercially available parental antibody	2097:2138	a commercially available parental antibody	2097:2138	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	9	71	theme	IRE1a	1536:1540	arg1	exception					1512:1520	the exception	1508:1520	the exception of bZIP28 and IRE1a	1508:1540	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	6	72	theme	homozygous	1009:1018	arg1	seeds					1023:1027	homozygous T4 seeds	1009:1027	homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs	1009:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	0	73	theme	monoclonal	71:80	arg1	mAb					92:94	mAb	92:94	mAb	92:94	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	0	73	theme	monoclonal	71:80	arg1	antibody					82:89	anti-cancer monoclonal antibody	59:89	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	8	74	theme	primary	1358:1364	arg1	lengths					1371:1377	COK plants primary root lengths	1347:1377	COK plants primary root lengths	1347:1377	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	5	75	theme	motif	768:772	arg1	sequence					774:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	13	76	theme	plasmon	1949:1955	arg1	resonance					1957:1965	Surface plasmon resonance	1941:1965	Surface plasmon resonance analyses	1941:1974	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	7	77	theme	mAb	1278:1280	arg1	CO					1282:1283	KDEL mAb CO	1273:1283	KDEL mAb CO (CO plant)	1273:1294	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	77	theme	mAb	1278:1280	arg1	plant					1289:1293	CO plant	1286:1293	CO plant	1286:1293	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	14	78	theme	mAb	2178:2180	arg1	CO					2182:2183	mAb CO	2178:2183	mAb CO	2178:2183	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	3	79	theme	transgenic	457:466	arg1	system					480:485	a transgenic Arabidopsis system	455:485	a transgenic Arabidopsis system	455:485	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	1	80	theme	synthesis	214:222	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	80	theme	synthesis	214:222	arg1	reticulum					171:179	The endoplasmic reticulum	155:179	The endoplasmic reticulum (ER)	155:184	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	80	theme	synthesis	214:222	arg1	secretion					238:246	secretion	238:246	secretion	238:246	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	80	theme	synthesis	214:222	arg1	folding					225:231	folding	225:231	folding	225:231	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	80	theme	synthesis	214:222	arg1	site					198:201	the main site	189:201	the main site of protein synthesis, folding, and secretion to other organelles	189:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	6	81	theme	cancer	1100:1105	arg1	mAbs					1107:1110	anti-colorectal cancer mAbs	1084:1110	anti-colorectal cancer mAbs	1084:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	11	82	theme	immunohistochemical	1784:1802	arg1	analyses					1804:1811	immunohistochemical analyses	1784:1811	immunohistochemical analyses	1784:1811	Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses.
30248125	1	83	theme	folding	225:231	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	83	theme	folding	225:231	arg1	reticulum					171:179	The endoplasmic reticulum	155:179	The endoplasmic reticulum (ER)	155:184	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	83	theme	folding	225:231	arg1	secretion					238:246	secretion	238:246	secretion	238:246	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	83	theme	folding	225:231	arg1	folding					225:231	folding	225:231	folding	225:231	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	83	theme	folding	225:231	arg1	site					198:201	the main site	189:201	the main site of protein synthesis, folding, and secretion to other organelles	189:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	7	84	theme	mAb	1258:1260	arg1	CO					1262:1263	mAb CO	1258:1263	mAb CO	1258:1263	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	14	85	theme	N-glycosylation	2141:2155	arg1	analysis					2157:2164	N-glycosylation analysis	2141:2164	N-glycosylation analysis	2141:2164	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	15	86	from	lines	2538:2542	arg1	media					2555:2559	culture media	2547:2559	culture media	2547:2559	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	10	87	theme	higher	1682:1687	arg1	expression					1689:1698	up to five times higher expression and mAb amounts	1665:1714	expression	1689:1698	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	5	88	theme	heavy	818:822	arg1	chain					824:828	the heavy chain	814:828	the heavy chain	814:828	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	0	89	theme	recombinant	47:57	arg1	CO17-1A					97:103	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	6	90	theme	KDEL	1162:1165	arg1	tagging					1167:1173	KDEL tagging	1162:1173	KDEL tagging	1162:1173	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	7	91	with	CO	1282:1283	arg1	COK					1321:1323	KDEL mAb COK	1312:1323	KDEL mAb COK (COK plant)	1312:1335	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	91	with	CO	1282:1283	arg1	plant					1330:1334	COK plant	1326:1334	COK plant	1326:1334	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	14	92	theme	glycan	2306:2311	arg1	residues					2313:2320	the plant specific glycan residues	2287:2320	the plant specific glycan residues	2287:2320	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	3	93	theme	monoclonal	526:535	arg1	mAbs					549:552	mAbs	549:552	mAbs	549:552	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	3	93	theme	monoclonal	526:535	arg1	antibodies					537:546	anti-cancer monoclonal antibodies	514:546	anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2	514:605	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	11	94	theme	Enhanced	1729:1736	arg1	expression					1738:1747	Enhanced expression	1729:1747	Enhanced expression in mAb COK plants	1729:1765	Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses.
30248125	10	95	dep	blot	1605:1608	arg1	analyses					1623:1630	analyses	1623:1630	analyses	1623:1630	Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants.
30248125	6	96	theme	floral	940:945	arg1	transformation					951:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation	917:964	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	15	97	theme	growth	2361:2366	arg1	reduction					2342:2350	the reduction	2338:2350	the reduction of plant growth and biomass induced by ER retention signal peptide	2338:2417	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	9	98	theme	ER	1478:1479	arg1	genes					1496:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	0	99	theme	stress	138:143	arg1	response					145:152	plant stress response	132:152	plant stress response	132:152	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	13	100	theme	better	2042:2047	arg1	activities					2057:2066	equivalent or slightly better binding activities	2019:2066	equivalent or slightly better binding activities	2019:2066	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	15	101	theme	transgenic	2527:2536	arg1	lines					2538:2542	transgenic lines	2527:2542	transgenic lines on culture media	2527:2559	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	1	102	theme	main	193:196	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	102	theme	main	193:196	arg1	reticulum					171:179	The endoplasmic reticulum	155:179	The endoplasmic reticulum (ER)	155:184	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	102	theme	main	193:196	arg1	secretion					238:246	secretion	238:246	secretion	238:246	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	102	theme	main	193:196	arg1	folding					225:231	folding	225:231	folding	225:231	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	1	102	theme	main	193:196	arg1	site					198:201	the main site	189:201	the main site of protein synthesis, folding, and secretion to other organelles	189:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	8	103	theme	CO	1406:1407	arg1	plants					1409:1414	CO plants	1406:1414	CO plants	1406:1414	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	4	104	theme	Monoclonal	608:617	arg1	mAb					629:631	mAb	629:631	mAb	629:631	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	4	104	theme	Monoclonal	608:617	arg1	antibody					619:626	Monoclonal antibody	608:626	Monoclonal antibody (mAb) CO17-1A	608:640	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	11	105	theme	COK	1756:1758	arg1	plants					1760:1765	mAb COK plants	1752:1765	mAb COK plants	1752:1765	Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses.
30248125	12	106	theme	tissues	1870:1876	arg1	area					1857:1860	the area	1853:1860	the area of leaf tissues	1853:1876	mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas.
30248125	13	107	theme	antigen	2071:2077	arg1	EpCAM					2079:2083	antigen EpCAM	2071:2083	antigen EpCAM	2071:2083	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	14	108	theme	plant	2291:2295	arg1	residues					2313:2320	the plant specific glycan residues	2287:2320	the plant specific glycan residues	2287:2320	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	3	109	theme	tumor-associated	574:589	arg1	GA733-2					599:605	the tumor-associated antigen GA733-2	570:605	the tumor-associated antigen GA733-2	570:605	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	8	110	theme	Arabidopsis	1435:1445	arg1	plants					1447:1452	non-transgenic Arabidopsis plants	1420:1452	non-transgenic Arabidopsis plants	1420:1452	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	7	111	theme	COK	1326:1328	arg1	COK					1321:1323	KDEL mAb COK	1312:1323	KDEL mAb COK (COK plant)	1312:1335	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	111	theme	COK	1326:1328	arg1	plant					1330:1334	COK plant	1326:1334	COK plant	1326:1334	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	0	112	theme	antibody	82:89	arg1	CO17-1A					97:103	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	6	113	used	used	1117:1120	arg2	seeds					1023:1027	homozygous T4 seeds	1009:1027	homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs	1009:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	5	114	theme	anti-colorectal	840:854	arg1	mAbs					863:866	anti-colorectal cancer mAbs	840:866	anti-colorectal cancer mAbs	840:866	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	14	115	theme	oligo-mannose	2246:2258	arg1	structure					2269:2277	an oligo-mannose N-glycan structure	2243:2277	an oligo-mannose N-glycan structure	2243:2277	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	5	116	theme	retention	735:743	arg1	sequence					774:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence	728:781	The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production.
30248125	15	117	theme	biomass	2372:2378	arg1	reduction					2342:2350	the reduction	2338:2350	the reduction of plant growth and biomass induced by ER retention signal peptide	2338:2417	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	9	118	theme	Most	1473:1476	arg1	genes					1496:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	2	119	theme	plant	433:437	arg1	diseases					439:446	plant diseases	433:446	plant diseases	433:446	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	4	120	theme	tumor-associated	654:669	arg1	epitope					671:677	a tumor-associated epitope	652:677	a tumor-associated epitope expressed on the colorectal cancer cell surface	652:725	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	15	121	dep	in	2436:2437	arg1	vitro					2439:2443	vitro	2439:2443	vitro	2439:2443	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	13	122	theme	mAb	2001:2003	arg1	COK					2005:2007	mAb CO and mAb COK	1990:2007	COK	2005:2007	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	9	123	theme	stress-related	1481:1494	arg1	genes					1496:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes	1473:1500	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	14	124	contain	had	2185:2187	arg1	CO					2182:2183	mAb CO	2178:2183	mAb CO	2178:2183	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	14	124	contain	had	2185:2187	arg2	residues					2204:2211	plant specific residues	2189:2211	plant specific residues	2189:2211	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	0	125	theme	mAb	113:115	arg1	expression					117:126	mAb expression	113:126	mAb expression	113:126	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	16	126	theme	peptide	2625:2631	arg1	approach					2649:2656	an effective approach	2636:2656	an effective approach for enhancing the yields of recombinant proteins in vivo	2636:2713	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	16	126	theme	peptide	2625:2631	arg1	fusion					2595:2600	the fusion	2591:2600	the fusion of ER retention signal peptide	2591:2631	Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
30248125	0	127	theme	reticulum	12:20	arg1	motif					32:36	Endoplasmic reticulum retention motif	0:36	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	0:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	6	128	theme	transgenic	1043:1052	arg1	plants					1066:1071	transgenic Arabidopsis plants	1043:1071	transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs	1043:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	4	129	theme	cancer	707:712	arg1	surface					719:725	the colorectal cancer cell surface	692:725	the colorectal cancer cell surface	692:725	Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface.
30248125	14	130	theme	specific	2195:2202	arg1	residues					2204:2211	plant specific residues	2189:2211	plant specific residues	2189:2211	N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues.
30248125	2	131	theme	excessive	328:336	arg1	accumulation					338:349	excessive accumulation	328:349	excessive accumulation of unfolded and misfolded proteins	328:384	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	1	132	theme	other	251:255	arg1	organelles					257:266	other organelles	251:266	other organelles	251:266	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	2	133	theme	proteins	377:384	arg1	accumulation					338:349	excessive accumulation	328:349	excessive accumulation of unfolded and misfolded proteins	328:384	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	12	134	theme	mAb	1814:1816	arg1	COK					1818:1820	mAb COK	1814:1820	mAb COK	1814:1820	mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas.
30248125	6	135	theme	T4	1020:1021	arg1	seeds					1023:1027	homozygous T4 seeds	1009:1027	homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs	1009:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	0	136	theme	anti-cancer	59:69	arg1	mAb					92:94	mAb	92:94	mAb	92:94	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	0	136	theme	anti-cancer	59:69	arg1	antibody					82:89	anti-cancer monoclonal antibody	59:89	recombinant anti-cancer monoclonal antibody (mAb) CO17-1A	47:103	Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response.
30248125	8	137	theme	plants	1351:1356	arg1	lengths					1371:1377	COK plants primary root lengths	1347:1377	COK plants primary root lengths	1347:1377	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	13	138	theme	Surface	1941:1947	arg1	resonance					1957:1965	Surface plasmon resonance	1941:1965	Surface plasmon resonance analyses	1941:1974	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30248125	15	139	theme	initial	2505:2511	arg1	screening					2513:2521	the initial screening	2501:2521	the initial screening for transgenic lines on culture media	2501:2559	In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media.
30248125	7	140	theme	KDEL	1273:1276	arg1	CO					1282:1283	KDEL mAb CO	1273:1283	KDEL mAb CO (CO plant)	1273:1294	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	7	140	theme	KDEL	1273:1276	arg1	plant					1289:1293	CO plant	1286:1293	CO plant	1286:1293	Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant).
30248125	8	141	theme	root	1366:1369	arg1	lengths					1371:1377	COK plants primary root lengths	1347:1377	COK plants primary root lengths	1347:1377	However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media.
30248125	2	142	theme	ER	289:290	arg1	capacity					273:280	The capacity	269:280	The capacity of the ER to process proteins	269:310	The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases.
30248125	1	143	theme	protein	206:212	arg1	synthesis					214:222	protein synthesis	206:222	protein synthesis	206:222	The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles.
30248125	9	144	theme	COK	1563:1565	arg1	plants					1567:1572	COK plants	1563:1572	COK plants	1563:1572	Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants.
30248125	6	145	theme	Arabidopsis	1054:1064	arg1	plants					1066:1071	transgenic Arabidopsis plants	1043:1071	transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs	1043:1110	Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging.
30248125	3	146	theme	Arabidopsis	468:478	arg1	system					480:485	a transgenic Arabidopsis system	455:485	a transgenic Arabidopsis system	455:485	Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2.
30248125	13	147	theme	available	2112:2120	arg1	antibody					2131:2138	a commercially available parental antibody	2097:2138	a commercially available parental antibody	2097:2138	Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody.
30439436	4	0	theme	tensile	511:517	arg1	strength					519:526	the tensile strength	507:526	the tensile strength	507:526	When the concentration of GO reached 2 mg/ml, the tensile strength was 25.28 MPa reaching the maximum.
30439436	7	1	theme	packaging	1004:1012	arg1	field					1014:1018	the food packaging field	995:1018	the food packaging field	995:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	5	2	dep	concentration	583:595	arg1	GO					580:581	GO	580:581	GO	580:581	The increase of GO concentration caused the enhancement of film's moisture resistance.
30439436	7	3	theme	films	1072:1076	arg1	research					1036:1043	the research	1032:1043	the research of biodegradable composite films	1032:1076	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	2	4	theme	film	321:324	arg1	performance					292:302	the performance	288:302	the performance of the composite film	288:324	The effects of GO with different concentrations on the performance of the composite film were studied.
30439436	7	5	dep	adding	912:917	arg1	GO					919:920	GO	919:920	GO	919:920	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	6	6	theme	film	868:871	arg1	stability					851:859	the thermal stability	839:859	the thermal stability of the film	839:871	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	2	7	theme	composite	311:319	arg1	film					321:324	the composite film	307:324	the composite film	307:324	The effects of GO with different concentrations on the performance of the composite film were studied.
30439436	3	8	theme	concentration	364:376	arg1	increase					349:356	the increase	345:356	the increase of GO concentration	345:376	With the increase of GO concentration, the thickness increased, the tensile strength increased first and then decreased.
30439436	5	9	theme	resistance	639:648	arg1	enhancement					608:618	the enhancement	604:618	the enhancement of film's moisture resistance	604:648	The increase of GO concentration caused the enhancement of film's moisture resistance.
30439436	5	10	theme	moisture	630:637	arg1	resistance					639:648	film's moisture resistance	623:648	film's moisture resistance	623:648	The increase of GO concentration caused the enhancement of film's moisture resistance.
30439436	7	11	theme	biodegradable	1048:1060	arg1	films					1072:1076	biodegradable composite films	1048:1076	biodegradable composite films	1048:1076	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	3	12	dep	increased	425:433	arg1	decreased					450:458	decreased	450:458	increased first and then decreased	425:458	With the increase of GO concentration, the thickness increased, the tensile strength increased first and then decreased.
30439436	5	13	theme	concentration	583:595	arg1	increase					568:575	The increase	564:575	The increase of GO concentration	564:595	The increase of GO concentration caused the enhancement of film's moisture resistance.
30439436	0	14	theme	Physical	0:7	arg1	properties					9:18	Physical properties	0:18	Physical properties	0:18	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	2	15	from	effects	241:247	arg1	performance					292:302	the performance	288:302	the performance of the composite film	288:324	The effects of GO with different concentrations on the performance of the composite film were studied.
30439436	3	16	theme	tensile	408:414	arg1	strength					416:423	the tensile strength	404:423	the tensile strength	404:423	With the increase of GO concentration, the thickness increased, the tensile strength increased first and then decreased.
30439436	6	17	theme	film	762:765	arg1	composition					743:753	the composition	739:753	the composition of the film	739:765	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	1	18	theme	starch/polyvinyl	116:131	arg1	film					166:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	1	19	theme	starch/PVA	218:227	arg1	matrix					229:234	starch/PVA matrix	218:234	starch/PVA matrix	218:234	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	0	20	theme	structural	24:33	arg1	characterization					35:50	structural characterization	24:50	structural characterization	24:50	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	7	21	theme	composite	893:901	arg1	films					903:907	composite films	893:907	composite films	893:907	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	7	22	theme	films	903:907	arg1	preparation					878:888	The preparation	874:888	The preparation of composite films by adding GO	874:920	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	1	23	theme	alcohol/graphene	133:148	arg1	film					166:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	7	24	from	application	963:973	arg1	field					1014:1018	the food packaging field	995:1018	the food packaging field	995:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	6	25	theme	thermal	843:849	arg1	stability					851:859	the thermal stability	839:859	the thermal stability of the film	839:871	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	1	26	theme	oxide	150:154	arg1	film					166:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	2	27	theme	different	260:268	arg1	concentrations					270:283	different concentrations	260:283	different concentrations	260:283	The effects of GO with different concentrations on the performance of the composite film were studied.
30439436	7	28	from	nanomaterials	978:990	arg1	field					1014:1018	the food packaging field	995:1018	the food packaging field	995:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	4	29	theme	GO	487:488	arg1	concentration					470:482	the concentration	466:482	the concentration of GO	466:488	When the concentration of GO reached 2 mg/ml, the tensile strength was 25.28 MPa reaching the maximum.
30439436	7	30	theme	nanomaterials	978:990	arg1	application					963:973	the application	959:973	the application of nanomaterials in the food packaging field	959:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	3	31	dep	concentration	364:376	arg1	GO					361:362	GO	361:362	GO	361:362	With the increase of GO concentration, the thickness increased, the tensile strength increased first and then decreased.
30439436	2	32	theme	GO	252:253	arg1	effects					241:247	The effects	237:247	The effects of GO with different concentrations on the performance of the composite film	237:324	The effects of GO with different concentrations on the performance of the composite film were studied.
30439436	1	33	theme	composite	156:164	arg1	film					166:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film	112:169	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	0	34	theme	starch/polyvinyl	55:70	arg1	properties					9:18	Physical properties	0:18	Physical properties	0:18	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	0	34	theme	starch/polyvinyl	55:70	arg1	characterization					35:50	structural characterization	24:50	structural characterization	24:50	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	6	35	theme	agglomeration	813:825	arg1	phenomenon					792:801	the phenomenon	788:801	the phenomenon of matrix agglomeration	788:825	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	7	36	theme	composite	1062:1070	arg1	films					1072:1076	biodegradable composite films	1048:1076	biodegradable composite films	1048:1076	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	6	37	theme	TGA	673:675	arg1	analysis					677:684	TGA analysis	673:684	TGA analysis	673:684	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	6	38	theme	matrix	806:811	arg1	agglomeration					813:825	matrix agglomeration	806:825	matrix agglomeration	806:825	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	7	39	theme	food	999:1002	arg1	field					1014:1018	the food packaging field	995:1018	the food packaging field	995:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
30439436	0	40	theme	composite	95:103	arg1	films					105:109	oxide composite films	89:109	oxide composite films	89:109	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	0	41	theme	oxide	89:93	arg1	films					105:109	oxide composite films	89:109	oxide composite films	89:109	Physical properties and structural characterization of starch/polyvinyl alcohol/graphene oxide composite films.
30439436	6	42	theme	GO	721:722	arg1	addition					709:716	the addition	705:716	the addition of GO	705:722	Through FTIR, SEM and TGA analysis, it was found that the addition of GO did not change the composition of the film, but it could change the phenomenon of matrix agglomeration and improve the thermal stability of the film.
30439436	1	43	theme	casting	187:193	arg1	method					195:200	casting method	187:200	casting method	187:200	The starch/polyvinyl alcohol/graphene oxide composite film was prepared by casting method by adding GO to starch/PVA matrix.
30439436	7	44	from	field	1014:1018	arg1	application					963:973	the application	959:973	the application of nanomaterials in the food packaging field	959:1018	The preparation of composite films by adding GO could improve the properties, expand the application of nanomaterials in the food packaging field, and expand the research of biodegradable composite films.
31771981	5	0	theme	streamlined	829:839	arg1	method					850:855	a streamlined MS-based method	827:855	a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	827:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	7	1	theme	transglycosylases	1582:1598	arg1	activity					1536:1543	the activity	1532:1543	the activity of amidases, deacetylases, and lytic transglycosylases	1532:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	4	2	theme	PG	764:765	arg1	composition					767:777	PG composition	764:777	PG composition	764:777	Conventional methods for analyzing PG composition are complex and time-consuming.
31771981	5	3	located	present	1077:1083	arg2	present					1077:1083	present	1077:1083	present	1077:1083	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	3	located	present	1077:1083	arg1	abundance					1093:1101	high abundance	1088:1101	high abundance	1088:1101	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	3	located	present	1077:1083	arg2	assembly					1054:1061	a core assembly	1047:1061	a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1047:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	4	theme	MS-based	841:848	arg1	method					850:855	a streamlined MS-based method	827:855	a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	827:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	8	5	theme	work	1621:1624	arg1	results					1605:1611	The results	1601:1611	The results of this work	1601:1624	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	8	6	dep	changing	1891:1898	arg1	response					1879:1886	response	1879:1886	response	1879:1886	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	1	7	theme	bacterial	178:186	arg1	wall					193:196	the bacterial cell wall	174:196	the bacterial cell wall	174:196	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	8	8	theme	first	1639:1643	arg1	evidence					1645:1652	first evidence	1639:1652	first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1639:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	1	9	theme	N-acetylmuramic	282:296	arg1	acid					298:301	N-acetylmuramic acid	282:301	N-acetylmuramic acid	282:301	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	7	10	theme	modifications	1493:1505	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	8	11	theme	PG	1860:1861	arg1	fluctuations					1863:1874	global PG fluctuations	1853:1874	global PG fluctuations	1853:1874	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	6	12	theme	growth	1287:1292	arg1	states					1294:1299	growth states	1287:1299	growth states	1287:1299	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	7	13	link	Biofilm-derived	1339:1353	arg1	PG					1364:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	1	14	theme	acid	298:301	arg1	disaccharide					242:253	a repeating β-1,4-linked disaccharide	217:253	a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide	217:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	5	15	theme	core	1049:1052	arg1	present					1077:1083	present	1077:1083	present	1077:1083	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	15	theme	core	1049:1052	arg1	assembly					1054:1061	a core assembly	1047:1061	a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1047:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	8	16	theme	de-N-acetylated	1657:1671	arg1	muropeptides					1673:1684	de-N-acetylated muropeptides	1657:1684	de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1657:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	8	17	theme	reproducible	1751:1762	arg1	workflow					1764:1771	a robust and reproducible workflow	1738:1771	a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1738:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	7	18	theme	adaptive	1355:1362	arg1	PG					1364:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	8	19	theme	global	1853:1858	arg1	fluctuations					1863:1874	global PG fluctuations	1853:1874	global PG fluctuations	1853:1874	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	1	20	theme	critical	152:159	arg1	Peptidoglycan					128:140	Peptidoglycan	128:140	Peptidoglycan (PG)	128:145	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	1	20	theme	critical	152:159	arg1	component					161:169	a critical component	150:169	a critical component of the bacterial cell wall	150:196	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	5	21	dep	aeruginosa	1022:1031	arg1	identified					1036:1045	identified	1036:1045	identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1036:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	7	22	theme	acid	1450:1453	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	1	23	theme	cell	188:191	arg1	wall					193:196	the bacterial cell wall	174:196	the bacterial cell wall	174:196	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	7	24	theme	lytic	1576:1580	arg1	transglycosylases					1582:1598	lytic transglycosylases	1576:1598	lytic transglycosylases	1576:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	7	25	theme	deacetylases	1558:1569	arg1	activity					1536:1543	the activity	1532:1543	the activity of amidases, deacetylases, and lytic transglycosylases	1532:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	1	26	theme	wall	193:196	arg1	Peptidoglycan					128:140	Peptidoglycan	128:140	Peptidoglycan (PG)	128:145	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	1	26	theme	wall	193:196	arg1	component					161:169	a critical component	150:169	a critical component of the bacterial cell wall	150:196	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	8	27	from	evidence	1645:1652	arg1	aeruginosa					1694:1703	P. aeruginosa	1691:1703	P. aeruginosa	1691:1703	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	5	28	attach	present	1077:1083	arg2	present					1077:1083	present	1077:1083	present	1077:1083	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	28	attach	present	1077:1083	arg1	abundance					1093:1101	high abundance	1088:1101	high abundance	1088:1101	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	28	attach	present	1077:1083	arg2	assembly					1054:1061	a core assembly	1047:1061	a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1047:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	7	29	theme	significant	1377:1387	arg1	changes					1389:1395	significant changes	1377:1395	significant changes	1377:1395	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	3	30	theme	important	611:619	arg1	functions					621:629	important functions	611:629	important functions	611:629	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	5	31	from	abundance	1093:1101	arg1	present					1077:1083	present	1077:1083	present	1077:1083	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	31	from	abundance	1093:1101	arg1	assembly					1054:1061	a core assembly	1047:1061	a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1047:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	4	32	theme	Conventional	729:740	arg1	methods					742:748	Conventional methods	729:748	Conventional methods for analyzing PG composition	729:777	Conventional methods for analyzing PG composition are complex and time-consuming.
31771981	1	33	link	β-1,4-linked	229:240	arg1	disaccharide					242:253	a repeating β-1,4-linked disaccharide	217:253	a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide	217:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	7	34	theme	amidases	1548:1555	arg1	activity					1536:1543	the activity	1532:1543	the activity of amidases, deacetylases, and lytic transglycosylases	1532:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	8	35	dep	muropeptides	1673:1684	arg1	offers					1731:1736	offers	1731:1736	offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1731:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	2	36	theme	considerable	461:472	arg1	maturation					474:483	maturation	474:483	maturation	474:483	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	6	37	theme	physiological	1316:1328	arg1	changes					1330:1336	physiological changes	1316:1336	physiological changes	1316:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	5	38	with	analysis	884:891	arg1	approaches					924:933	statistical 1D annotation approaches	898:933	statistical 1D annotation approaches	898:933	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	39	theme	annotation	913:922	arg1	approaches					924:933	statistical 1D annotation approaches	898:933	statistical 1D annotation approaches	898:933	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	0	40	theme	compositional	25:37	arg1	changes					39:45	compositional changes	25:45	compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa	25:125	Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
31771981	8	41	theme	external	1921:1928	arg1	stimuli					1930:1936	external stimuli	1921:1936	external stimuli	1921:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	5	42	theme	PG	1066:1067	arg1	present					1077:1083	present	1077:1083	present	1077:1083	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	5	42	theme	PG	1066:1067	arg1	assembly					1054:1061	a core assembly	1047:1061	a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states	1047:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	7	43	from	changes	1521:1527	arg1	activity					1536:1543	the activity	1532:1543	the activity of amidases, deacetylases, and lytic transglycosylases	1532:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	7	44	theme	amino	1444:1448	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	1	45	theme	conserved	326:334	arg1	peptide					341:347	a highly conserved stem peptide	317:347	a highly conserved stem peptide	317:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	2	46	theme	stressors	574:582	arg1	presence					548:555	presence	548:555	presence	548:555	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	2	46	theme	stressors	574:582	arg1	phase					539:543	the growth phase	528:543	the growth phase	528:543	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	0	47	dep	biofilm-	72:79	arg1	aeruginosa					116:125	Pseudomonas aeruginosa	104:125	Pseudomonas aeruginosa	104:125	Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
31771981	6	48	from	amounts	1243:1249	arg1	assembly					1207:1214	an adaptive PG assembly	1192:1214	an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes	1192:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	48	from	amounts	1243:1249	arg1	present					1224:1230	present	1224:1230	present	1224:1230	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	8	49	from	composition	1803:1813	arg1	samples					1818:1824	samples	1818:1824	samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1818:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	6	50	located	present	1224:1230	arg1	amounts					1243:1249	smaller amounts	1235:1249	smaller amounts	1235:1249	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	50	located	present	1224:1230	arg2	present					1224:1230	present	1224:1230	present	1224:1230	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	50	located	present	1224:1230	arg2	assembly					1207:1214	an adaptive PG assembly	1192:1214	an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes	1192:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	1	51	theme	stem	336:339	arg1	peptide					341:347	a highly conserved stem peptide	317:347	a highly conserved stem peptide	317:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	2	52	theme	environmental	560:572	arg1	stressors					574:582	environmental stressors	560:582	environmental stressors	560:582	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	3	53	theme	cell	676:679	arg1	elongation/division					681:699	cell elongation/division	676:699	cell elongation/division	676:699	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	5	54	theme	statistical	898:908	arg1	approaches					924:933	statistical 1D annotation approaches	898:933	statistical 1D annotation approaches	898:933	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	1	55	theme	repeating	219:227	arg1	disaccharide					242:253	a repeating β-1,4-linked disaccharide	217:253	a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide	217:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	8	56	theme	PG	1800:1801	arg1	composition					1803:1813	PG composition	1800:1813	PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1800:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	8	57	theme	growth	1900:1905	arg1	conditions					1907:1916	growth conditions	1900:1916	growth conditions	1900:1916	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	5	58	theme	differential	871:882	arg1	analysis					884:891	differential analysis	871:891	differential analysis with statistical 1D annotation approaches	871:933	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	3	59	theme	diverse	634:640	arg1	processes					642:650	diverse processes	634:650	diverse processes	634:650	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	3	59	theme	diverse	634:640	arg1	resistance					717:726	antibiotic resistance	706:726	antibiotic resistance	706:726	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	3	59	theme	diverse	634:640	arg1	turnover					666:673	PG turnover	663:673	PG turnover	663:673	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	3	59	theme	diverse	634:640	arg1	elongation/division					681:699	cell elongation/division	676:699	cell elongation/division	676:699	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	6	60	from	present	1224:1230	arg1	amounts					1243:1249	smaller amounts	1235:1249	smaller amounts	1235:1249	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	7	61	theme	planktonic-derived	1411:1428	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	7	61	theme	planktonic-derived	1411:1428	arg1	PG					1430:1431	planktonic-derived PG	1411:1431	planktonic-derived PG	1411:1431	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	7	62	theme	peptide	1481:1487	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	8	63	from	aeruginosa	1694:1703	arg1	evidence					1645:1652	first evidence	1639:1652	first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1639:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	8	63	from	aeruginosa	1694:1703	arg1	muropeptides					1673:1684	de-N-acetylated muropeptides	1657:1684	de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1657:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	6	64	attach	present	1224:1230	arg1	amounts					1243:1249	smaller amounts	1235:1249	smaller amounts	1235:1249	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	64	attach	present	1224:1230	arg2	present					1224:1230	present	1224:1230	present	1224:1230	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	64	attach	present	1224:1230	arg2	assembly					1207:1214	an adaptive PG assembly	1192:1214	an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes	1192:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	5	65	theme	high	1088:1091	arg1	abundance					1093:1101	high abundance	1088:1101	high abundance	1088:1101	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	6	66	theme	smaller	1235:1241	arg1	amounts					1243:1249	smaller amounts	1235:1249	smaller amounts	1235:1249	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	1	67	theme	β-1,4-linked	229:240	arg1	disaccharide					242:253	a repeating β-1,4-linked disaccharide	217:253	a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide	217:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31771981	7	68	link	planktonic-derived	1411:1428	arg1	substitutions					1455:1467	amino acid substitutions	1444:1467	amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases	1444:1598	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	7	68	link	planktonic-derived	1411:1428	arg1	PG					1430:1431	planktonic-derived PG	1411:1431	planktonic-derived PG	1411:1431	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	3	69	theme	antibiotic	706:715	arg1	resistance					717:726	antibiotic resistance	706:726	antibiotic resistance	706:726	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	5	70	theme	1D	910:911	arg1	approaches					924:933	statistical 1D annotation approaches	898:933	statistical 1D annotation approaches	898:933	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	8	71	used	used	1838:1841	arg2	samples					1818:1824	samples	1818:1824	samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1818:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	2	72	theme	Gram-negative	353:365	arg1	bacteria					367:374	Gram-negative bacteria	353:374	Gram-negative bacteria	353:374	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	8	73	theme	robust	1740:1745	arg1	workflow					1764:1771	a robust and reproducible workflow	1738:1771	a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1738:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	2	74	theme	growth	532:537	arg1	phase					539:543	the growth phase	528:543	the growth phase	528:543	In Gram-negative bacteria, PG is assembled in the cytoplasm and exported into the periplasm where it undergoes considerable maturation, modification, or degradation depending on the growth phase or presence of environmental stressors.
31771981	5	75	theme	growth	1158:1163	arg1	states					1165:1170	the two growth states	1150:1170	the two growth states	1150:1170	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	7	76	theme	stem	1476:1479	arg1	peptide					1481:1487	the stem peptide	1472:1487	the stem peptide	1472:1487	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	8	77	theme	muropeptides	1673:1684	arg1	evidence					1645:1652	first evidence	1639:1652	first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli	1639:1936	The results of this work also provide first evidence of de-N-acetylated muropeptides from P. aeruginosa The method developed here offers a robust and reproducible workflow for accurately determining PG composition in samples that can be used to assess global PG fluctuations in response to changing growth conditions or external stimuli.
31771981	6	78	theme	PG	1204:1205	arg1	assembly					1207:1214	an adaptive PG assembly	1192:1214	an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes	1192:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	78	theme	PG	1204:1205	arg1	present					1224:1230	present	1224:1230	present	1224:1230	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	7	79	theme	Biofilm-derived	1339:1353	arg1	PG					1364:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG	1339:1365	Biofilm-derived adaptive PG exhibited significant changes compared with planktonic-derived PG, including amino acid substitutions of the stem peptide and modifications that indicate changes in the activity of amidases, deacetylases, and lytic transglycosylases.
31771981	0	80	from	changes	39:45	arg1	peptidoglycan					50:62	peptidoglycan	50:62	peptidoglycan	50:62	Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
31771981	3	81	theme	PG	663:664	arg1	turnover					666:673	PG turnover	663:673	PG turnover	663:673	These modifications serve important functions in diverse processes, including PG turnover, cell elongation/division, and antibiotic resistance.
31771981	6	82	theme	adaptive	1195:1202	arg1	assembly					1207:1214	an adaptive PG assembly	1192:1214	an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes	1192:1336	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	6	82	theme	adaptive	1195:1202	arg1	present					1224:1230	present	1224:1230	present	1224:1230	We also identified an adaptive PG assembly that is present in smaller amounts and fluctuates considerably between growth states in response to physiological changes.
31771981	5	83	from	present	1077:1083	arg1	abundance					1093:1101	high abundance	1088:1101	high abundance	1088:1101	We present here a streamlined MS-based method that combines differential analysis with statistical 1D annotation approaches to quantitatively compare PGs produced in planktonic- and biofilm-cultured Pseudomonas aeruginosa We identified a core assembly of PG that is present in high abundance and that does not significantly differ between the two growth states.
31771981	1	84	theme	N-acetylglucosamine	258:276	arg1	disaccharide					242:253	a repeating β-1,4-linked disaccharide	217:253	a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide	217:347	Peptidoglycan (PG) is a critical component of the bacterial cell wall and is composed of a repeating β-1,4-linked disaccharide of N-acetylglucosamine and N-acetylmuramic acid appended with a highly conserved stem peptide.
31349450	6	0	theme	morphology	1005:1014	arg1	observation					1016:1026	surface morphology observation	997:1026	surface morphology observation	997:1026	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	3	1	theme	oxidation	651:659	arg1	degrees					661:667	three oxidation degrees	645:667	three oxidation degrees (AHA40, AHA50 and AHA60)	645:692	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	12	2	theme	tissue	2210:2215	arg1	applications					2229:2240	further tissue engineering applications	2202:2240	further tissue engineering applications	2202:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	1	3	theme	different	228:236	arg1	degrees					248:254	different oxidation degrees	228:254	different oxidation degrees	228:254	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	9	4	theme	AHA	1562:1564	arg1	degree					1552:1557	the oxidation degree	1538:1557	the oxidation degree of AHA	1538:1564	On the other hand, when raising the oxidation degree of AHA, the hydrophilicity of the gels decreased and less time was required to form the gel matrix.
31349450	7	5	theme	in	1163:1164	arg1	cytotoxicity					1172:1183	in vitro cytotoxicity	1163:1183	in vitro cytotoxicity	1163:1183	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	1	6	theme	degrees	248:254	arg1	effect					218:223	the effect	214:223	the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	214:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	4	7	theme	amino	786:790	arg1	groups					792:797	amino groups	786:797	amino groups of NOCC	786:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	7	theme	amino	786:790	arg1	NOCC					802:805	NOCC	802:805	NOCC	802:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	7	theme	amino	786:790	arg1	AHA					778:780	AHA	778:780	AHA	778:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	10	8	theme	wound	1804:1808	arg1	healing					1810:1816	wound healing	1804:1816	wound healing	1804:1816	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	11	9	theme	pore	2008:2011	arg1	size					2013:2016	favorable pore size	1998:2016	favorable pore size	1998:2016	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	11	10	theme	wound	1910:1914	arg1	healing					1916:1922	skin wound healing	1905:1922	skin wound healing applications	1905:1935	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	11	11	theme	favorable	1998:2006	arg1	size					2013:2016	favorable pore size	1998:2016	favorable pore size	1998:2016	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	9	12	theme	gels	1593:1596	arg1	hydrophilicity					1571:1584	the hydrophilicity	1567:1584	the hydrophilicity of the gels	1567:1596	On the other hand, when raising the oxidation degree of AHA, the hydrophilicity of the gels decreased and less time was required to form the gel matrix.
31349450	6	13	theme	degradation	1059:1069	arg1	test					1071:1074	in vitro degradation test	1050:1074	in vitro degradation test	1050:1074	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	10	14	dep	in	1681:1682	arg1	vitro					1684:1688	vitro	1684:1688	vitro	1684:1688	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	1	15	theme	chitosan-hyaluronic	364:382	arg1	hydrogel					395:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel	339:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	339:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	11	16	theme	healing	1916:1922	arg1	applications					1924:1935	skin wound healing applications	1905:1935	skin wound healing applications	1905:1935	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	1	17	theme	hydrogel	395:402	arg1	biocompatibility					319:334	biocompatibility	319:334	biocompatibility	319:334	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	1	17	theme	hydrogel	395:402	arg1	properties					304:313	physical properties	295:313	physical properties	295:313	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	3	18	dep	degrees	661:667	arg1	AHA50					677:681	AHA50	677:681	AHA50	677:681	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	3	18	dep	degrees	661:667	arg1	AHA40					670:674	AHA40	670:674	AHA40	670:674	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	3	18	dep	degrees	661:667	arg1	AHA60					687:691	AHA60	687:691	AHA60	687:691	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	1	19	from	effect	218:223	arg1	biocompatibility					319:334	biocompatibility	319:334	biocompatibility	319:334	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	1	19	from	effect	218:223	arg1	properties					304:313	physical properties	295:313	physical properties	295:313	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	1	20	theme	skin	408:411	arg1	healing					419:425	skin wound healing	408:425	skin wound healing applications	408:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	10	21	theme	obtained	1672:1679	arg1	results					1702:1708	the obtained in vitro and in vivo results	1668:1708	the obtained in vitro and in vivo results	1668:1708	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	11	22	theme	high	1981:1984	arg1	uniformity					1986:1995	high uniformity	1981:1995	high uniformity	1981:1995	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	7	23	theme	composite	1280:1288	arg1	biocompatibility					1256:1271	the biocompatibility	1252:1271	the biocompatibility of the composite	1252:1288	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	1	24	theme	healing	419:425	arg1	applications					427:438	skin wound healing applications	408:438	skin wound healing applications	408:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	11	25	theme	NOCC-AHA40	1860:1869	arg1	hydrogel					1875:1882	NOCC-AHA40 5:5 hydrogel	1860:1882	NOCC-AHA40 5:5 hydrogel	1860:1882	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	5	26	theme	NOCC	868:871	arg1	ratios					884:889	three NOCC:AHA volume ratios	862:889	three NOCC:AHA volume ratios (3:7, 5:5 and 7:3)	862:908	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	8	27	theme	hydrogels	1404:1412	arg1	hydrophobicity					1372:1385	hydrophobicity	1372:1385	hydrophobicity	1372:1385	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	8	27	theme	hydrogels	1404:1412	arg1	homogeneity					1356:1366	homogeneity	1356:1366	homogeneity	1356:1366	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	10	28	theme	in	1694:1695	arg1	results					1702:1708	the obtained in vitro and in vivo results	1668:1708	the obtained in vitro and in vivo results	1668:1708	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	5	29	theme	polysaccharide	818:831	arg1	derivatives					833:843	the polysaccharide derivatives	814:843	the polysaccharide derivatives	814:843	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	0	30	dep	hydrogels	118:126	arg1	based					128:132	based	128:132	hydrogels based on chitosan and hyaluronic acid	118:164	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	9	31	theme	gel	1647:1649	arg1	matrix					1651:1656	the gel matrix	1643:1656	the gel matrix	1643:1656	On the other hand, when raising the oxidation degree of AHA, the hydrophilicity of the gels decreased and less time was required to form the gel matrix.
31349450	8	32	theme	slower	1481:1486	arg1	rate					1500:1503	slower degradation rate	1481:1503	slower degradation rate	1481:1503	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	11	33	theme	superior	1956:1963	arg1	morphology					1965:1974	superior morphology	1956:1974	superior morphology	1956:1974	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	5	34	theme	additional	956:965	arg1	cross-linker					967:978	any additional cross-linker	952:978	any additional cross-linker	952:978	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	2	35	theme	chitosan	513:520	arg1	chain					504:508	the polymer chain	492:508	the polymer chain of chitosan	492:520	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	8	36	theme	pore	1443:1446	arg1	walls					1448:1452	their pore walls	1437:1452	their pore walls	1437:1452	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	10	37	theme	healing	1810:1816	arg1	process					1818:1824	cell proliferation, cell attachment and wound healing process	1764:1824	cell proliferation, cell attachment and wound healing process	1764:1824	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	12	38	theme	effective	2180:2188	arg1	bio-glue					2190:2197	an effective bio-glue	2177:2197	an effective bio-glue in further tissue engineering applications	2177:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	38	theme	effective	2180:2188	arg1	matrix					2092:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	5	39	theme	AHA	873:875	arg1	ratios					884:889	three NOCC:AHA volume ratios	862:889	three NOCC:AHA volume ratios (3:7, 5:5 and 7:3)	862:908	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	2	40	theme	Carboxymethyl	441:453	arg1	groups					455:460	Carboxymethyl groups	441:460	Carboxymethyl groups (-CH2COOH)	441:471	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	2	40	theme	Carboxymethyl	441:453	arg1	-CH2COOH					463:470	-CH2COOH	463:470	-CH2COOH	463:470	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	1	41	theme	ratios	267:272	arg1	effect					218:223	the effect	214:223	the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	214:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	10	42	theme	oxidation	1731:1739	arg1	degree					1741:1746	lower oxidation degree	1725:1746	lower oxidation degree of AHA	1725:1753	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	0	43	theme	degree	24:29	arg1	effect					4:9	The effect	0:9	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking	0:116	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	1	44	theme	components	277:286	arg1	ratios					267:272	volume ratios	260:272	volume ratios of components	260:286	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	1	44	theme	components	277:286	arg1	degrees					248:254	different oxidation degrees	228:254	different oxidation degrees	228:254	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	5	45	theme	composite	918:926	arg1	hydrogels					928:936	composite hydrogels	918:936	composite hydrogels	918:936	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	8	46	theme	degradation	1488:1498	arg1	rate					1500:1503	slower degradation rate	1481:1503	slower degradation rate	1481:1503	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	3	47	theme	hyaluronic	618:627	arg1	acid					629:632	aldehyde hyaluronic acid	609:632	aldehyde hyaluronic acid (AHA)	609:638	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	3	47	theme	hyaluronic	618:627	arg1	AHA					635:637	AHA	635:637	AHA	635:637	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	4	48	theme	AHA	778:780	arg1	groups					792:797	amino groups	786:797	amino groups of NOCC	786:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	48	theme	AHA	778:780	arg1	groups					768:773	aldehyde groups	759:773	aldehyde groups of AHA	759:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	48	theme	AHA	778:780	arg1	NOCC					802:805	NOCC	802:805	NOCC	802:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	48	theme	AHA	778:780	arg1	AHA					778:780	AHA	778:780	AHA	778:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	0	49	theme	volume	35:40	arg1	ratio					42:46	volume ratio	35:46	volume ratio	35:46	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	7	50	theme	healing	1203:1209	arg1	evaluations					1211:1221	in vitro cytotoxicity and in vivo wound healing evaluations	1163:1221	in vitro cytotoxicity and in vivo wound healing evaluations	1163:1221	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	1	51	dep	in	342:343	arg1	situ					345:348	situ	345:348	situ	345:348	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	8	52	theme	NOCC	1346:1349	arg1	volume					1336:1341	the volume	1332:1341	the volume of NOCC	1332:1349	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	9	53	theme	other	1513:1517	arg1	hand					1519:1522	the other hand	1509:1522	the other hand	1509:1522	On the other hand, when raising the oxidation degree of AHA, the hydrophilicity of the gels decreased and less time was required to form the gel matrix.
31349450	6	54	theme	wettability	1032:1042	arg1	test					1044:1047	wettability test	1032:1047	wettability test	1032:1047	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	6	55	dep	in	1050:1051	arg1	vitro					1053:1057	vitro	1053:1057	vitro	1053:1057	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	9	56	theme	oxidation	1542:1550	arg1	degree					1552:1557	the oxidation degree	1538:1557	the oxidation degree of AHA	1538:1564	On the other hand, when raising the oxidation degree of AHA, the hydrophilicity of the gels decreased and less time was required to form the gel matrix.
31349450	10	57	theme	cell	1784:1787	arg1	attachment					1789:1798	cell attachment	1784:1798	cell attachment	1784:1798	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	12	58	theme	other	2147:2151	arg1	factors					2153:2159	other factors	2147:2159	other factors	2147:2159	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	4	59	theme	NOCC	802:805	arg1	groups					792:797	amino groups	786:797	amino groups of NOCC	786:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	59	theme	NOCC	802:805	arg1	groups					768:773	aldehyde groups	759:773	aldehyde groups of AHA	759:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	59	theme	NOCC	802:805	arg1	NOCC					802:805	NOCC	802:805	NOCC	802:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	59	theme	NOCC	802:805	arg1	AHA					778:780	AHA	778:780	AHA	778:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	6	60	theme	surface	997:1003	arg1	observation					1016:1026	surface morphology observation	997:1026	surface morphology observation	997:1026	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	10	61	theme	proliferation	1769:1781	arg1	process					1818:1824	cell proliferation, cell attachment and wound healing process	1764:1824	cell proliferation, cell attachment and wound healing process	1764:1824	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	12	62	theme	NOCC-AHA	2079:2086	arg1	system					2136:2141	a delivery system	2125:2141	a delivery system for other factors	2125:2159	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	62	theme	NOCC-AHA	2079:2086	arg1	bio-glue					2190:2197	an effective bio-glue	2177:2197	an effective bio-glue in further tissue engineering applications	2177:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	62	theme	NOCC-AHA	2079:2086	arg1	matrix					2092:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	1	63	theme	oxidation	238:246	arg1	degrees					248:254	different oxidation degrees	228:254	different oxidation degrees	228:254	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	7	64	theme	wound	1197:1201	arg1	healing					1203:1209	in vivo wound healing	1189:1209	in vivo wound healing	1189:1209	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	11	65	theme	5:5	1871:1873	arg1	hydrogel					1875:1882	NOCC-AHA40 5:5 hydrogel	1860:1882	NOCC-AHA40 5:5 hydrogel	1860:1882	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	8	66	gly	homogeneity	1356:1366	arg1	hydrogels					1404:1412	the resulting hydrogels	1390:1412	the resulting hydrogels	1390:1412	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	11	67	theme	suitable	2022:2029	arg1	density					2031:2037	suitable density	2022:2037	suitable density	2022:2037	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	6	68	theme	rheological	1080:1090	arg1	analysis					1092:1099	rheological analysis	1080:1099	rheological analysis	1080:1099	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	10	69	dep	in	1694:1695	arg1	vivo					1697:1700	vivo	1697:1700	vivo	1697:1700	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	11	70	theme	skin	1905:1908	arg1	healing					1916:1922	skin wound healing	1905:1922	skin wound healing applications	1905:1935	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	0	71	theme	cross-linking	104:116	arg1	applications					80:91	applications	80:91	applications	80:91	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	0	71	theme	cross-linking	104:116	arg1	properties					65:74	properties	65:74	properties	65:74	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	12	72	theme	further	2202:2208	arg1	applications					2229:2240	further tissue engineering applications	2202:2240	further tissue engineering applications	2202:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	1	73	theme	acid-based	384:393	arg1	hydrogel					395:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel	339:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	339:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	3	74	theme	Hyaluronic	570:579	arg1	acid					581:584	Hyaluronic acid	570:584	Hyaluronic acid	570:584	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	1	75	theme	research	187:194	arg1	purpose					171:177	The purpose	167:177	The purpose of this research	167:194	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	12	76	theme	engineering	2217:2227	arg1	applications					2229:2240	further tissue engineering applications	2202:2240	further tissue engineering applications	2202:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	1	77	theme	physical	295:302	arg1	properties					304:313	physical properties	295:313	physical properties	295:313	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	10	78	theme	in	1681:1682	arg1	results					1702:1708	the obtained in vitro and in vivo results	1668:1708	the obtained in vitro and in vivo results	1668:1708	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	8	79	theme	resulting	1394:1402	arg1	hydrogels					1404:1412	the resulting hydrogels	1390:1412	the resulting hydrogels	1390:1412	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	11	80	contain	possesses	1946:1954	arg1	it					1943:1944	it	1943:1944	it	1943:1944	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	11	80	contain	possesses	1946:1954	arg2	morphology					1965:1974	superior morphology	1956:1974	superior morphology	1956:1974	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	0	81	theme	hyaluronic	150:159	arg1	acid					161:164	hyaluronic acid	150:164	hyaluronic acid	150:164	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	1	82	theme	wound	413:417	arg1	healing					419:425	skin wound healing	408:425	skin wound healing applications	408:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	2	83	theme	Carboxymethyl	539:551	arg1	Chitosan					553:560	Carboxymethyl Chitosan	539:560	Carboxymethyl Chitosan	539:560	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	2	83	theme	Carboxymethyl	539:551	arg1	N					533:533	N	533:533	N	533:533	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	0	84	dep	in	96:97	arg1	situ					99:102	situ	99:102	situ	99:102	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	4	85	theme	base	738:741	arg1	linkage					743:749	Schiff base linkage	731:749	Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC	731:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	0	86	theme	components	51:60	arg1	degree					24:29	oxidation degree	14:29	oxidation degree	14:29	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	0	86	theme	components	51:60	arg1	ratio					42:46	volume ratio	35:46	volume ratio	35:46	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	10	87	theme	lower	1725:1729	arg1	degree					1741:1746	lower oxidation degree	1725:1746	lower oxidation degree of AHA	1725:1753	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	2	88	theme	polymer	496:502	arg1	chain					504:508	the polymer chain	492:508	the polymer chain of chitosan	492:520	Carboxymethyl groups (-CH2COOH) were introduced to the polymer chain of chitosan, producing N,O - Carboxymethyl Chitosan (NOCC).
31349450	5	89	dep	ratios	884:889	arg1	3:7					892:894	3:7	892:894	3:7	892:894	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	5	89	dep	ratios	884:889	arg1	7:3					905:907	7:3	905:907	7:3	905:907	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	5	89	dep	ratios	884:889	arg1	5:5					897:899	5:5	897:899	5:5	897:899	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	0	90	from	effect	4:9	arg1	applications					80:91	applications	80:91	applications	80:91	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	0	90	from	effect	4:9	arg1	properties					65:74	properties	65:74	properties	65:74	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	7	91	dep	in	1189:1190	arg1	vivo					1192:1195	vivo	1192:1195	vivo	1192:1195	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	12	92	theme	delivery	2127:2134	arg1	system					2136:2141	a delivery system	2125:2141	a delivery system for other factors	2125:2159	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	92	theme	delivery	2127:2134	arg1	matrix					2092:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	0	93	theme	in	96:97	arg1	cross-linking					104:116	in situ cross-linking	96:116	in situ cross-linking	96:116	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	1	94	theme	in	342:343	arg1	hydrogel					395:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel	339:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	339:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	12	95	used	used	2117:2120	arg2	bio-glue					2190:2197	an effective bio-glue	2177:2197	an effective bio-glue in further tissue engineering applications	2177:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	95	used	used	2117:2120	arg2	matrix					2092:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	95	used	used	2117:2120	arg2	system					2136:2141	a delivery system	2125:2141	a delivery system for other factors	2125:2159	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	1	96	theme	cross-linking	350:362	arg1	hydrogel					395:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel	339:402	an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications	339:438	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	7	97	dep	in	1163:1164	arg1	vitro					1166:1170	vitro	1166:1170	vitro	1166:1170	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	6	98	theme	FT-IR	981:985	arg1	analysis					987:994	FT-IR analysis	981:994	FT-IR analysis	981:994	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	11	99	theme	appropriate	2050:2060	arg1	wettability					2062:2072	appropriate wettability	2050:2072	appropriate wettability	2050:2072	It is also concluded that NOCC-AHA40 5:5 hydrogel is most suitable for skin wound healing applications since it possesses superior morphology with high uniformity, favorable pore size and suitable density along with appropriate wettability.
31349450	12	100	theme	gel	2088:2090	arg1	system					2136:2141	a delivery system	2125:2141	a delivery system for other factors	2125:2159	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	100	theme	gel	2088:2090	arg1	bio-glue					2190:2197	an effective bio-glue	2177:2197	an effective bio-glue in further tissue engineering applications	2177:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	12	100	theme	gel	2088:2090	arg1	matrix					2092:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix	2075:2097	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	0	101	theme	oxidation	14:22	arg1	degree					24:29	oxidation degree	14:29	oxidation degree	14:29	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	1	102	theme	volume	260:265	arg1	ratios					267:272	volume ratios	260:272	volume ratios of components	260:286	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	5	103	theme	volume	877:882	arg1	ratios					884:889	three NOCC:AHA volume ratios	862:889	three NOCC:AHA volume ratios (3:7, 5:5 and 7:3)	862:908	Then, the polysaccharide derivatives were combined at three NOCC:AHA volume ratios (3:7, 5:5 and 7:3) to form composite hydrogels without using any additional cross-linker.
31349450	1	104	dep	properties	304:313	arg1	the					291:293	the	291:293	the	291:293	The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications.
31349450	12	105	from	bio-glue	2190:2197	arg1	applications					2229:2240	further tissue engineering applications	2202:2240	further tissue engineering applications	2202:2240	The NOCC-AHA gel matrix is expected to be used as a delivery system for other factors and employed as an effective bio-glue in further tissue engineering applications.
31349450	3	106	theme	aldehyde	609:616	arg1	acid					629:632	aldehyde hyaluronic acid	609:632	aldehyde hyaluronic acid (AHA)	609:638	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	3	106	theme	aldehyde	609:616	arg1	AHA					635:637	AHA	635:637	AHA	635:637	Hyaluronic acid was oxidized to obtain aldehyde hyaluronic acid (AHA) with three oxidation degrees (AHA40, AHA50 and AHA60).
31349450	4	107	theme	aldehyde	759:766	arg1	groups					768:773	aldehyde groups	759:773	aldehyde groups of AHA	759:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	107	theme	aldehyde	759:766	arg1	NOCC					802:805	NOCC	802:805	NOCC	802:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	4	107	theme	aldehyde	759:766	arg1	AHA					778:780	AHA	778:780	AHA	778:780	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	0	108	theme	ratio	42:46	arg1	effect					4:9	The effect	0:9	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking	0:116	The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid.
31349450	6	109	theme	in	1050:1051	arg1	test					1071:1074	in vitro degradation test	1050:1074	in vitro degradation test	1050:1074	FT-IR analysis, surface morphology observation and wettability test, in vitro degradation test and rheological analysis were carried out to characterize the hydrogels.
31349450	10	110	theme	cell	1764:1767	arg1	proliferation					1769:1781	cell proliferation	1764:1781	cell proliferation	1764:1781	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	8	111	dep	homogeneity	1356:1366	arg1	the					1352:1354	the	1352:1354	the	1352:1354	Our findings showed that when increasing the volume of NOCC, the homogeneity and hydrophobicity of the resulting hydrogels were also improved and their pore walls became thicker, leading to slower degradation rate.
31349450	7	112	theme	in	1189:1190	arg1	healing					1203:1209	in vivo wound healing	1189:1209	in vivo wound healing	1189:1209	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	10	113	theme	AHA	1751:1753	arg1	degree					1741:1746	lower oxidation degree	1725:1746	lower oxidation degree of AHA	1725:1753	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
31349450	4	114	theme	Schiff	731:736	arg1	linkage					743:749	Schiff base linkage	731:749	Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC	731:805	The gelation was induced by forming Schiff base linkage between aldehyde groups of AHA and amino groups of NOCC.
31349450	7	115	theme	cytotoxicity	1172:1183	arg1	evaluations					1211:1221	in vitro cytotoxicity and in vivo wound healing evaluations	1163:1221	in vitro cytotoxicity and in vivo wound healing evaluations	1163:1221	Additionally, in vitro cytotoxicity and in vivo wound healing evaluations were also conducted to study the biocompatibility of the composite.
31349450	10	116	theme	attachment	1789:1798	arg1	process					1818:1824	cell proliferation, cell attachment and wound healing process	1764:1824	cell proliferation, cell attachment and wound healing process	1764:1824	Besides, the obtained in vitro and in vivo results indicated that lower oxidation degree of AHA supports cell proliferation, cell attachment and wound healing process better.
30716696	5	0	theme	characterization	797:812	arg1	results					814:820	The characterization results	793:820	The characterization results	793:820	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	4	1	theme	alginate	717:724	arg1	beads					736:740	the drug-loaded alginate composite beads	701:740	the drug-loaded alginate composite beads	701:740	Ultimately, the drug-loaded alginate composite beads were successfully fabricated by external gelation.
30716696	1	2	theme	interfacial	154:164	arg1	assembly					166:173	the interfacial assembly	150:173	the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method	150:257	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	0	3	theme	alginate	90:97	arg1	beads					99:103	alginate beads	90:103	alginate beads for hydrophobic drug delivery	90:133	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	2	4	theme	sulfuric	361:368	arg1	hydrolysis					375:384	sulfuric acid hydrolysis	361:384	sulfuric acid hydrolysis of biosynthesized bacterial cellulose	361:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	9	5	theme	composite	1576:1584	arg1	beads					1586:1590	the resultant alginate composite beads	1553:1590	the resultant alginate composite beads	1553:1590	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	6	6	theme	Pickering	1051:1059	arg1	emulsions					1061:1069	the Pickering emulsions	1047:1069	the Pickering emulsions	1047:1069	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	0	7	theme	hydrophobic	109:119	arg1	delivery					126:133	hydrophobic drug delivery	109:133	hydrophobic drug delivery	109:133	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	4	8	theme	external	774:781	arg1	gelation					783:790	external gelation	774:790	external gelation	774:790	Ultimately, the drug-loaded alginate composite beads were successfully fabricated by external gelation.
30716696	7	9	theme	composite	1266:1274	arg1	beads					1276:1280	the alginate composite beads	1253:1280	the alginate composite beads formed by external gelation	1253:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	7	10	theme	interfacial	1185:1195	arg1	assembly					1197:1204	The interfacial assembly	1181:1204	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation	1181:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	7	11	theme	loading	1323:1329	arg1	release					1345:1351	the loading and sustained release	1319:1351	the loading and sustained release of alfacalcidol	1319:1367	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	2	12	theme	acid	370:373	arg1	hydrolysis					375:384	sulfuric acid hydrolysis	361:384	sulfuric acid hydrolysis of biosynthesized bacterial cellulose	361:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	8	13	from	beads	1493:1497	arg1	mechanism					1448:1456	the release mechanism	1436:1456	the release mechanism of alfacalcidol from the composite beads	1436:1497	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	4	14	theme	drug-loaded	705:715	arg1	beads					736:740	the drug-loaded alginate composite beads	701:740	the drug-loaded alginate composite beads	701:740	Ultimately, the drug-loaded alginate composite beads were successfully fabricated by external gelation.
30716696	8	15	theme	release	1374:1380	arg1	curves					1382:1387	The release curves	1370:1387	The release curves	1370:1387	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	6	16	theme	alginate	1162:1169	arg1	solution					1171:1178	alginate solution	1162:1178	alginate solution	1162:1178	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	2	17	theme	bacterial	404:412	arg1	cellulose					414:422	biosynthesized bacterial cellulose	389:422	biosynthesized bacterial cellulose	389:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	8	18	theme	Korsmeyer	1409:1417	arg1	model					1426:1430	Korsmeyer Peppas model	1409:1430	Korsmeyer Peppas model	1409:1430	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	0	19	theme	drug	121:124	arg1	delivery					126:133	hydrophobic drug delivery	109:133	hydrophobic drug delivery	109:133	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	5	20	theme	Pickering	952:960	arg1	emulsions					962:970	Pickering emulsions	952:970	Pickering emulsions	952:970	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	7	21	theme	beads	1276:1280	arg1	assembly					1197:1204	The interfacial assembly	1181:1204	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation	1181:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	1	22	theme	amphiphilic	178:188	arg1	BCNs					224:227	BCNs	224:227	BCNs	224:227	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	1	22	theme	amphiphilic	178:188	arg1	nanocrystals					210:221	amphiphilic bacterial cellulose nanocrystals	178:221	amphiphilic bacterial cellulose nanocrystals (BCNs)	178:228	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	5	23	theme	good	851:854	arg1	property					866:873	good colloidal property	851:873	good colloidal property	851:873	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	7	24	theme	external	1292:1299	arg1	gelation					1301:1308	external gelation	1292:1308	external gelation	1292:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	9	25	theme	good	1623:1626	arg1	capabilities					1628:1639	good capabilities	1623:1639	good capabilities for osteoblast differentiation	1623:1670	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	1	26	theme	bacterial	190:198	arg1	BCNs					224:227	BCNs	224:227	BCNs	224:227	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	1	26	theme	bacterial	190:198	arg1	nanocrystals					210:221	amphiphilic bacterial cellulose nanocrystals	178:221	amphiphilic bacterial cellulose nanocrystals (BCNs)	178:228	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	5	27	theme	colloidal	856:864	arg1	property					866:873	good colloidal property	851:873	good colloidal property	851:873	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	8	28	theme	release	1440:1446	arg1	mechanism					1448:1456	the release mechanism	1436:1456	the release mechanism of alfacalcidol from the composite beads	1436:1497	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	0	29	theme	Pickering	58:66	arg1	droplets					78:85	Pickering emulsions droplets	58:85	Pickering emulsions droplets	58:85	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	1	30	theme	cellulose	200:208	arg1	BCNs					224:227	BCNs	224:227	BCNs	224:227	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	1	30	theme	cellulose	200:208	arg1	nanocrystals					210:221	amphiphilic bacterial cellulose nanocrystals	178:221	amphiphilic bacterial cellulose nanocrystals (BCNs)	178:228	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	0	31	theme	cellulose	24:32	arg1	nanocrystals					34:45	bacterial cellulose nanocrystals	14:45	bacterial cellulose nanocrystals	14:45	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	3	32	theme	oil-in-water	554:565	arg1	emulsions					577:585	The oil-in-water Pickering emulsions	550:585	The oil-in-water Pickering emulsions	550:585	The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.
30716696	1	33	theme	nanocrystals	210:221	arg1	assembly					166:173	the interfacial assembly	150:173	the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method	150:257	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	0	34	theme	bacterial	14:22	arg1	nanocrystals					34:45	bacterial cellulose nanocrystals	14:45	bacterial cellulose nanocrystals	14:45	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	6	35	theme	oil-water	1016:1024	arg1	interface					1026:1034	the oil-water interface	1012:1034	the oil-water interface	1012:1034	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	3	36	theme	Pickering	567:575	arg1	emulsions					577:585	The oil-in-water Pickering emulsions	550:585	The oil-in-water Pickering emulsions	550:585	The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.
30716696	6	37	theme	Ostwald	1117:1123	arg1	ripening					1125:1132	Ostwald ripening	1117:1132	Ostwald ripening	1117:1132	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	6	38	from	interface	1026:1034	arg1	adsorption					990:999	The irreversible adsorption	973:999	The irreversible adsorption of BCNs at the oil-water interface	973:1034	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	7	39	theme	alginate	1257:1264	arg1	beads					1276:1280	the alginate composite beads	1253:1280	the alginate composite beads formed by external gelation	1253:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	2	40	used	used	527:530	arg2	alfacalcidol					489:500	alfacalcidol	489:500	alfacalcidol	489:500	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	40	used	used	527:530	arg2	drug					483:486	the model drug	473:486	the model drug	473:486	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	40	used	used	527:530	arg2	phase					543:547	the oil phase	535:547	the oil phase	535:547	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	3	41	theme	ultrasonic	604:613	arg1	method					626:631	ultrasonic dispersion method	604:631	ultrasonic dispersion method	604:631	The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.
30716696	2	42	theme	biosynthesized	389:402	arg1	cellulose					414:422	biosynthesized bacterial cellulose	389:422	biosynthesized bacterial cellulose	389:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	5	43	theme	flocculated	890:900	arg1	network					909:915	flocculated fibril network	890:915	flocculated fibril network	890:915	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	0	44	theme	nanocrystals	34:45	arg1	Entrapment					0:9	Entrapment	0:9	Entrapment of bacterial cellulose nanocrystals	0:45	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	1	45	theme	alginate	313:320	arg1	drug					338:341	the alginate and hydrophobic drug	309:341	the alginate and hydrophobic drug	309:341	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	2	46	theme	oil	539:541	arg1	drug					483:486	the model drug	473:486	the model drug	473:486	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	46	theme	oil	539:541	arg1	phase					543:547	the oil phase	535:547	the oil phase	535:547	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	47	dep	used	428:431	arg1	whereas					465:471	whereas	465:471	whereas	465:471	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	48	used	used	428:431	arg2	emulsifiers					452:462	the particulate emulsifiers	436:462	the particulate emulsifiers	436:462	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	48	used	used	428:431	arg2	BCNs					344:347	BCNs	344:347	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose	344:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	5	49	theme	fibril	902:907	arg1	network					909:915	flocculated fibril network	890:915	flocculated fibril network	890:915	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	0	50	theme	emulsions	68:76	arg1	droplets					78:85	Pickering emulsions droplets	58:85	Pickering emulsions droplets	58:85	Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.
30716696	6	51	theme	BCNs	1004:1007	arg1	adsorption					990:999	The irreversible adsorption	973:999	The irreversible adsorption of BCNs at the oil-water interface	973:1034	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	9	52	theme	resultant	1557:1565	arg1	beads					1586:1590	the resultant alginate composite beads	1553:1590	the resultant alginate composite beads	1553:1590	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	7	53	theme	alfacalcidol	1356:1367	arg1	release					1345:1351	the loading and sustained release	1319:1351	the loading and sustained release of alfacalcidol	1319:1367	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	2	54	theme	model	477:481	arg1	drug					483:486	the model drug	473:486	the model drug	473:486	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	54	theme	model	477:481	arg1	phase					543:547	the oil phase	535:547	the oil phase	535:547	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	54	theme	model	477:481	arg1	alfacalcidol					489:500	alfacalcidol	489:500	alfacalcidol	489:500	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	1	55	theme	hydrophobic	326:336	arg1	drug					338:341	the alginate and hydrophobic drug	309:341	the alginate and hydrophobic drug	309:341	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	5	56	contain	possessed	841:849	arg1	BCNs					836:839	BCNs	836:839	BCNs	836:839	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	5	56	contain	possessed	841:849	arg2	property					866:873	good colloidal property	851:873	good colloidal property	851:873	The characterization results revealed that BCNs possessed good colloidal property and could form flocculated fibril network, which was beneficial to stabilize Pickering emulsions.
30716696	8	57	theme	composite	1483:1491	arg1	beads					1493:1497	the composite beads	1479:1497	the composite beads	1479:1497	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	4	58	theme	composite	726:734	arg1	beads					736:740	the drug-loaded alginate composite beads	701:740	the drug-loaded alginate composite beads	701:740	Ultimately, the drug-loaded alginate composite beads were successfully fabricated by external gelation.
30716696	9	59	theme	osteoblast	1645:1654	arg1	differentiation					1656:1670	osteoblast differentiation	1645:1670	osteoblast differentiation	1645:1670	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	7	60	theme	hydrogel	1234:1241	arg1	shells					1243:1248	the hydrogel shells	1230:1248	the hydrogel shells	1230:1248	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	7	61	theme	BCNs	1221:1224	arg1	assembly					1197:1204	The interfacial assembly	1181:1204	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation	1181:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	3	62	theme	dispersion	615:624	arg1	method					626:631	ultrasonic dispersion method	604:631	ultrasonic dispersion method	604:631	The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.
30716696	8	63	theme	alfacalcidol	1461:1472	arg1	mechanism					1448:1456	the release mechanism	1436:1456	the release mechanism of alfacalcidol from the composite beads	1436:1497	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	3	64	theme	alginate	670:677	arg1	solution					679:686	alginate solution	670:686	alginate solution	670:686	The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.
30716696	9	65	theme	low	1602:1604	arg1	cytotoxicity					1606:1617	low cytotoxicity	1602:1617	low cytotoxicity	1602:1617	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	8	66	theme	Peppas	1419:1424	arg1	model					1426:1430	Korsmeyer Peppas model	1409:1430	Korsmeyer Peppas model	1409:1430	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	1	67	theme	Pickering	233:241	arg1	method					252:257	Pickering emulsion method	233:257	Pickering emulsion method	233:257	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	9	68	theme	alginate	1567:1574	arg1	beads					1586:1590	the resultant alginate composite beads	1553:1590	the resultant alginate composite beads	1553:1590	In addition, the resultant alginate composite beads exhibited low cytotoxicity and good capabilities for osteoblast differentiation.
30716696	8	69	theme	non-Fickian	1517:1527	arg1	transport					1529:1537	non-Fickian transport	1517:1537	non-Fickian transport	1517:1537	The release curves were well fitted by Korsmeyer Peppas model and the release mechanism of alfacalcidol from the composite beads was attributed to non-Fickian transport.
30716696	2	70	theme	particulate	440:450	arg1	emulsifiers					452:462	the particulate emulsifiers	436:462	the particulate emulsifiers	436:462	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	70	theme	particulate	440:450	arg1	BCNs					344:347	BCNs	344:347	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose	344:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	2	71	theme	cellulose	414:422	arg1	hydrolysis					375:384	sulfuric acid hydrolysis	361:384	sulfuric acid hydrolysis of biosynthesized bacterial cellulose	361:422	BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CH2Cl2 was used as the oil phase.
30716696	1	72	theme	emulsion	243:250	arg1	method					252:257	Pickering emulsion method	233:257	Pickering emulsion method	233:257	In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug.
30716696	7	73	theme	amphiphilic	1209:1219	arg1	BCNs					1221:1224	amphiphilic BCNs	1209:1224	amphiphilic BCNs	1209:1224	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	6	74	theme	irreversible	977:988	arg1	adsorption					990:999	The irreversible adsorption	973:999	The irreversible adsorption of BCNs at the oil-water interface	973:1034	The irreversible adsorption of BCNs at the oil-water interface could make the Pickering emulsions preserve the droplets against coalescence and Ostwald ripening when they were dispersed in alginate solution.
30716696	7	75	theme	shells	1243:1248	arg1	assembly					1197:1204	The interfacial assembly	1181:1204	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation	1181:1308	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30716696	7	76	theme	sustained	1335:1343	arg1	release					1345:1351	the loading and sustained release	1319:1351	the loading and sustained release of alfacalcidol	1319:1367	The interfacial assembly of amphiphilic BCNs and the hydrogel shells of the alginate composite beads formed by external gelation achieved the loading and sustained release of alfacalcidol.
30535148	8	0	theme	nuclear	1156:1162	arg1	DNA					1174:1176	nuclear ribosomal DNA	1156:1176	nuclear ribosomal DNA	1156:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	14	1	dep	serology	1938:1945	arg1	AMCA					1957:1960	AMCA	1957:1960	AMCA	1957:1960	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	14	1	dep	serology	1938:1945	arg1	ACCA					1948:1951	ACCA	1948:1951	ACCA	1948:1951	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	2	2	with	Patients	214:221	arg1	colitis					239:245	ulcerative colitis	228:245	ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery)	228:315	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	2	with	Patients	214:221	arg1	UC					248:249	UC	248:249	UC	248:249	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	4	3	theme	sugar	584:588	arg1	consumption					590:600	dietary sugar consumption	576:600	dietary sugar consumption	576:600	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	0	4	with	Patients	84:91	arg1	Pouch					108:112	Ileo-Anal Pouch	98:112	Ileo-Anal Pouch	98:112	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	8	5	theme	DNA	1174:1176	arg1	region					1146:1151	the internal transcribed spacer 2 (ITS2) region	1105:1151	the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA	1105:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	14	6	theme	immune	2035:2040	arg1	response					2042:2049	a specific immune response	2024:2049	a specific immune response	2024:2049	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	12	7	dep	%	1580:1580	arg1	=					1596:1596	=	1596:1596	=	1596:1596	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	6	8	link	enzyme-linked	813:825	arg1	assay					841:845	enzyme-linked immunosorbent assay	813:845	enzyme-linked immunosorbent assay	813:845	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	1	9	theme	BACKGROUND	115:124	arg1	IBDs					155:158	IBDs	155:158	IBDs	155:158	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	1	9	theme	BACKGROUND	115:124	arg1	diseases					145:152	BACKGROUND Inflammatory bowel diseases	115:152	BACKGROUND Inflammatory bowel diseases (IBDs)	115:159	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	9	10	theme	pouch	1205:1209	arg1	patients					1211:1218	75 UC pouch patients	1199:1218	75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously	1199:1294	RESULTS We included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously.
30535148	0	11	theme	Starch	0:5	arg1	Consumption					7:17	Starch Consumption	0:17	Starch Consumption	0:17	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	9	12	theme	aged	1220:1223	arg1	patients					1211:1218	75 UC pouch patients	1199:1218	75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously	1199:1294	RESULTS We included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously.
30535148	8	13	theme	fungal	1034:1039	arg1	community					1041:1049	The fungal community	1030:1049	The fungal community in patients' fecal samples	1030:1076	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	13	14	theme	starch	1818:1823	arg1	consumption					1825:1835	starch consumption	1818:1835	starch consumption	1818:1835	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	14	15	theme	starch	1998:2003	arg1	intake					2005:2010	increased dietary starch intake	1980:2010	increased dietary starch intake	1980:2010	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	2	16	theme	pouch	302:306	arg1	anastomosis					289:299	ileo-anal anastomosis	279:299	ileo-anal anastomosis (pouch surgery)	279:315	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	16	theme	pouch	302:306	arg1	surgery					308:314	pouch surgery	302:314	pouch surgery	302:314	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	17	with	colitis	239:245	arg1	anastomosis					289:299	ileo-anal anastomosis	279:299	ileo-anal anastomosis (pouch surgery)	279:315	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	17	with	colitis	239:245	arg1	surgery					308:314	pouch surgery	302:314	pouch surgery	302:314	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	14	18	theme	increased	1980:1988	arg1	intake					2005:2010	increased dietary starch intake	1980:2010	increased dietary starch intake	1980:2010	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	12	19	theme	ACCA	1544:1547	arg1	titers					1549:1554	ACCA titers	1544:1554	ACCA titers	1544:1554	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	5	20	theme	METHODS	676:682	arg1	Patients					684:691	METHODS Patients	676:691	METHODS Patients	676:691	METHODS Patients were followed prospectively for 2 consecutive visits.
30535148	4	21	with	patients	605:612	arg1	evolution					636:644	the evolution	632:644	the evolution in antibody levels over time	632:673	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	4	21	with	patients	605:612	arg1	pouch					622:626	UC pouch	619:626	UC pouch	619:626	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	1	22	theme	Inflammatory	126:137	arg1	IBDs					155:158	IBDs	155:158	IBDs	155:158	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	1	22	theme	Inflammatory	126:137	arg1	diseases					145:152	BACKGROUND Inflammatory bowel diseases	115:152	BACKGROUND Inflammatory bowel diseases (IBDs)	115:159	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	13	23	theme	fecal	1766:1770	arg1	samples					1772:1778	fecal samples	1766:1778	fecal samples	1766:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	7	24	theme	frequency	1005:1013	arg1	questionnaire					1015:1027	a food frequency questionnaire	998:1027	a food frequency questionnaire	998:1027	Patients completed a food frequency questionnaire.
30535148	0	25	theme	Fungal	62:67	arg1	Composition					69:79	Fecal Fungal Composition	56:79	Fecal Fungal Composition	56:79	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	12	26	theme	starch	1489:1494	arg1	consumption					1496:1506	Higher starch consumption	1482:1506	Higher starch consumption	1482:1506	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	12	27	dep	=	1628:1628	arg1	%					1618:1618	0.7%	1615:1618	0.7%-9.1%	1615:1623	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	4	28	from	consumption	590:600	arg1	patients					605:612	patients	605:612	patients with UC pouch and the evolution in antibody levels over time	605:673	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	13	29	dep	=	1861:1861	arg1	r					1849:1849	Spearman's r	1838:1849	Spearman's r = 0.72	1838:1856	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	13	30	theme	relative	1724:1731	arg1	abundance					1733:1741	the relative abundance	1720:1741	the relative abundance of Candida albicans in fecal samples	1720:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	4	31	from	expression	561:570	arg1	patients					605:612	patients	605:612	patients with UC pouch and the evolution in antibody levels over time	605:673	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	4	32	theme	antiglycan	541:550	arg1	expression					561:570	antiglycan antibody expression	541:570	antiglycan antibody expression	541:570	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	14	33	theme	positive	1918:1925	arg1	serology					1938:1945	positive antiglycan serology	1918:1945	positive antiglycan serology (ACCA and AMCA)	1918:1961	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	9	34	theme	UC	1202:1203	arg1	patients					1211:1218	75 UC pouch patients	1199:1218	75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously	1199:1294	RESULTS We included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously.
30535148	11	35	theme	serologic	1450:1458	arg1	responses					1460:1468	positive serologic responses	1441:1468	positive serologic responses	1441:1468	Starch consumption was significantly higher in patients with positive serologic responses (P = 0.05).
30535148	10	36	theme	antiglycan	1357:1366	arg1	antibodies					1368:1377	antiglycan antibodies	1357:1377	antiglycan antibodies	1357:1377	Of these patients, 34.7% (n = 26) showed seropositivity for antiglycan antibodies.
30535148	13	37	theme	per-patient	1698:1708	arg1	change					1710:1715	The per-patient change	1694:1715	The per-patient change in the relative abundance of Candida albicans in fecal samples	1694:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	0	38	theme	Antiglycan	30:39	arg1	Antibodies					41:50	Antiglycan Antibodies	30:50	Antiglycan Antibodies	30:50	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	8	39	theme	internal	1109:1116	arg1	region					1146:1151	the internal transcribed spacer 2 (ITS2) region	1105:1151	the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA	1105:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	12	40	theme	Higher	1482:1487	arg1	consumption					1496:1506	Higher starch consumption	1482:1506	Higher starch consumption	1482:1506	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	14	41	theme	Starch	1883:1888	arg1	consumption					1890:1900	CONCLUSIONS Starch consumption	1871:1900	CONCLUSIONS Starch consumption	1871:1900	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	6	42	theme	carbohydrate	772:783	arg1	antibodies					785:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	12	43	theme	starch	1686:1691	arg1	increase					1666:1673	each 10-g increase	1656:1673	each 10-g increase of dietary starch	1656:1691	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	12	44	theme	dietary	1678:1684	arg1	starch					1686:1691	dietary starch	1678:1691	dietary starch	1678:1691	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	6	45	theme	enzyme-linked	813:825	arg1	assay					841:845	enzyme-linked immunosorbent assay	813:845	enzyme-linked immunosorbent assay	813:845	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	46	theme	following	751:759	arg1	antibodies					785:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	47	theme	anti-Saccharomyces	930:947	arg1	ASCA					961:964	ASCA	961:964	ASCA	961:964	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	47	theme	anti-Saccharomyces	930:947	arg1	cerevisiae					949:958	anti-Saccharomyces cerevisiae	930:958	anti-Saccharomyces cerevisiae (ASCA)	930:965	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	4	48	theme	UC	619:620	arg1	pouch					622:626	UC pouch	619:626	UC pouch	619:626	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	3	49	theme	sugars	488:493	arg1	consumption					465:475	their consumption	459:475	their consumption of dietary sugars	459:493	We hypothesized that patients' serologic responses were affected by their consumption of dietary sugars.
30535148	4	50	theme	dietary	576:582	arg1	consumption					590:600	dietary sugar consumption	576:600	dietary sugar consumption	576:600	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	11	51	theme	Starch	1380:1385	arg1	consumption					1387:1397	Starch consumption	1380:1397	Starch consumption	1380:1397	Starch consumption was significantly higher in patients with positive serologic responses (P = 0.05).
30535148	8	52	theme	ribosomal	1164:1172	arg1	DNA					1174:1176	nuclear ribosomal DNA	1156:1176	nuclear ribosomal DNA	1156:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	6	53	dep	detected	801:808	arg1	ACCA					866:869	ACCA	866:869	ACCA	866:869	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	antimannobioside					901:916	antimannobioside	901:916	antimannobioside (AMCA)	901:923	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	cerevisiae					949:958	anti-Saccharomyces cerevisiae	930:958	anti-Saccharomyces cerevisiae (ASCA)	930:965	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	AMCA					919:922	AMCA	919:922	AMCA	919:922	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	antilaminaribioside					873:891	antilaminaribioside	873:891	antilaminaribioside (ALCA)	873:898	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	antichitobioside					848:863	antichitobioside	848:863	antichitobioside (ACCA)	848:870	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	ASCA					961:964	ASCA	961:964	ASCA	961:964	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	6	53	dep	detected	801:808	arg1	ALCA					894:897	ALCA	894:897	ALCA	894:897	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	14	54	theme	specific	2026:2033	arg1	response					2042:2049	a specific immune response	2024:2049	a specific immune response	2024:2049	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	6	55	theme	immunosorbent	827:839	arg1	assay					841:845	enzyme-linked immunosorbent assay	813:845	enzyme-linked immunosorbent assay	813:845	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	2	56	theme	ileo-anal	279:287	arg1	anastomosis					289:299	ileo-anal anastomosis	279:299	ileo-anal anastomosis (pouch surgery)	279:315	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	56	theme	ileo-anal	279:287	arg1	surgery					308:314	pouch surgery	302:314	pouch surgery	302:314	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	4	57	theme	antibody	649:656	arg1	levels					658:663	antibody levels	649:663	antibody levels	649:663	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	12	58	dep	%	1618:1618	arg1	%					1623:1623	-9.1%	1619:1623	0.7%-9.1%	1615:1623	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	8	59	theme	spacer	1130:1135	arg1	region					1146:1151	the internal transcribed spacer 2 (ITS2) region	1105:1151	the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA	1105:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	1	60	theme	serologic	182:190	arg1	responses					192:200	serologic responses	182:200	serologic responses to glycans	182:211	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	12	61	dep	%	1612:1612	arg1	=					1628:1628	=	1628:1628	=	1628:1628	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	4	62	from	evolution	636:644	arg1	levels					658:663	antibody levels	649:663	antibody levels	649:663	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	11	63	with	patients	1427:1434	arg1	responses					1460:1468	positive serologic responses	1441:1468	positive serologic responses	1441:1468	Starch consumption was significantly higher in patients with positive serologic responses (P = 0.05).
30535148	6	64	dep	antichitobioside	848:863	arg1	antibodies					967:976	antibodies	967:976	antibodies	967:976	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	14	65	theme	dietary	1990:1996	arg1	intake					2005:2010	increased dietary starch intake	1980:2010	increased dietary starch intake	1980:2010	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	5	66	theme	consecutive	727:737	arg1	visits					739:744	2 consecutive visits	725:744	2 consecutive visits	725:744	METHODS Patients were followed prospectively for 2 consecutive visits.
30535148	2	67	theme	ulcerative	228:237	arg1	colitis					239:245	ulcerative colitis	228:245	ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery)	228:315	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	2	67	theme	ulcerative	228:237	arg1	UC					248:249	UC	248:249	UC	248:249	Patients with ulcerative colitis (UC) after proctocolectomy with ileo-anal anastomosis (pouch surgery) may develop inflammation (pouchitis) that resembles Crohn's disease (CD).
30535148	13	68	from	changes	1807:1813	arg1	consumption					1825:1835	starch consumption	1818:1835	starch consumption	1818:1835	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	14	69	with	patients	2054:2061	arg1	IBD					2068:2070	IBD	2068:2070	IBD	2068:2070	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	9	70	theme	pouch	1255:1259	arg1	surgery					1261:1267	pouch surgery	1255:1267	pouch surgery	1255:1267	RESULTS We included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously.
30535148	1	71	theme	bowel	139:143	arg1	IBDs					155:158	IBDs	155:158	IBDs	155:158	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	1	71	theme	bowel	139:143	arg1	diseases					145:152	BACKGROUND Inflammatory bowel diseases	115:152	BACKGROUND Inflammatory bowel diseases (IBDs)	115:159	BACKGROUND Inflammatory bowel diseases (IBDs) are characterized by serologic responses to glycans.
30535148	12	72	theme	higher	1528:1533	arg1	AMCA					1535:1538	higher AMCA	1528:1538	higher AMCA	1528:1538	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	4	73	from	pouch	622:626	arg1	levels					658:663	antibody levels	649:663	antibody levels	649:663	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	13	74	theme	albicans	1754:1761	arg1	abundance					1733:1741	the relative abundance	1720:1741	the relative abundance of Candida albicans in fecal samples	1720:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	7	75	theme	food	1000:1003	arg1	questionnaire					1015:1027	a food frequency questionnaire	998:1027	a food frequency questionnaire	998:1027	Patients completed a food frequency questionnaire.
30535148	13	76	theme	Candida	1746:1752	arg1	albicans					1754:1761	Candida albicans	1746:1761	Candida albicans	1746:1761	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	0	77	theme	Ileo-Anal	98:106	arg1	Pouch					108:112	Ileo-Anal Pouch	98:112	Ileo-Anal Pouch	98:112	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	8	78	theme	fecal	1064:1068	arg1	samples					1070:1076	patients' fecal samples	1054:1076	patients' fecal samples	1054:1076	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	13	79	from	abundance	1733:1741	arg1	samples					1772:1778	fecal samples	1766:1778	fecal samples	1766:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	3	80	theme	serologic	422:430	arg1	responses					432:440	patients' serologic responses	412:440	patients' serologic responses	412:440	We hypothesized that patients' serologic responses were affected by their consumption of dietary sugars.
30535148	4	81	theme	antibody	552:559	arg1	expression					561:570	antiglycan antibody expression	541:570	antiglycan antibody expression	541:570	This study analyzed the correlations between antiglycan antibody expression and dietary sugar consumption in patients with UC pouch and the evolution in antibody levels over time.
30535148	14	82	theme	antiglycan	1927:1936	arg1	serology					1938:1945	positive antiglycan serology	1918:1945	positive antiglycan serology (ACCA and AMCA)	1918:1961	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	11	83	theme	positive	1441:1448	arg1	responses					1460:1468	positive serologic responses	1441:1468	positive serologic responses	1441:1468	Starch consumption was significantly higher in patients with positive serologic responses (P = 0.05).
30535148	9	84	dep	RESULTS	1179:1185	arg1	included					1190:1197	included	1190:1197	included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously	1190:1294	RESULTS We included 75 UC pouch patients aged 45.2 ± 14 years who underwent pouch surgery 9.8 ± 6.7 years previously.
30535148	8	85	theme	transcribed	1118:1128	arg1	region					1146:1151	the internal transcribed spacer 2 (ITS2) region	1105:1151	the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA	1105:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	12	86	theme	%	1586:1586	arg1	%					1591:1591	0.8%-7.4%	1583:1591	0.8%-7.4%	1583:1591	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	0	87	theme	Fecal	56:60	arg1	Composition					69:79	Fecal Fungal Composition	56:79	Fecal Fungal Composition	56:79	Starch Consumption May Modify Antiglycan Antibodies and Fecal Fungal Composition in Patients With Ileo-Anal Pouch.
30535148	12	88	theme	10-g	1661:1664	arg1	increase					1666:1673	each 10-g increase	1656:1673	each 10-g increase of dietary starch	1656:1691	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	14	89	theme	CONCLUSIONS	1871:1881	arg1	consumption					1890:1900	CONCLUSIONS Starch consumption	1871:1900	CONCLUSIONS Starch consumption	1871:1900	CONCLUSIONS Starch consumption correlated with positive antiglycan serology (ACCA and AMCA), suggesting that increased dietary starch intake may promote a specific immune response in patients with IBD.
30535148	6	90	theme	antiglycan	761:770	arg1	antibodies					785:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies	747:794	The following antiglycan carbohydrate antibodies were detected by enzyme-linked immunosorbent assay: antichitobioside (ACCA), antilaminaribioside (ALCA), antimannobioside (AMCA), and anti-Saccharomyces cerevisiae (ASCA) antibodies.
30535148	3	91	theme	dietary	480:486	arg1	sugars					488:493	dietary sugars	480:493	dietary sugars	480:493	We hypothesized that patients' serologic responses were affected by their consumption of dietary sugars.
30535148	8	92	theme	ITS2	1140:1143	arg1	region					1146:1151	the internal transcribed spacer 2 (ITS2) region	1105:1151	the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA	1105:1176	The fungal community in patients' fecal samples was analyzed by sequencing the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA.
30535148	12	93	dep	=	1596:1596	arg1	%					1591:1591	0.8%-7.4%	1583:1591	0.8%-7.4%	1583:1591	Higher starch consumption was associated with higher AMCA and ACCA titers, which increased by 4.08% (0.8%-7.4%; P = 0.014) and 4.8% (0.7%-9.1%; P = 0.007), respectively, for each 10-g increase of dietary starch.
30535148	13	94	from	change	1710:1715	arg1	abundance					1733:1741	the relative abundance	1720:1741	the relative abundance of Candida albicans in fecal samples	1720:1778	The per-patient change in the relative abundance of Candida albicans in fecal samples correlated positively with changes in starch consumption (Spearman's r = 0.72; P = 0.012).
30535148	10	95	theme	=	1325:1325	arg1	%					1320:1320	34.7%	1316:1320	34.7% (n = 26)	1316:1329	Of these patients, 34.7% (n = 26) showed seropositivity for antiglycan antibodies.
30535148	10	95	theme	=	1325:1325	arg1	n					1323:1323	n = 26	1323:1328	n = 26	1323:1328	Of these patients, 34.7% (n = 26) showed seropositivity for antiglycan antibodies.
29437925	2	0	theme	polysaccharide	385:398	arg1	complex					400:406	a discrete polysaccharide complex	374:406	a discrete polysaccharide complex	374:406	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	2	0	theme	polysaccharide	385:398	arg1	holdfast					413:420	the holdfast	409:420	the holdfast	409:420	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	4	1	theme	enzymatic	634:642	arg1	assays					644:649	enzymatic assays	634:649	enzymatic assays	634:649	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	10	2	theme	strong	1935:1940	arg1	bioadhesives					1957:1968	strong, biocompatible bioadhesives	1935:1968	strong, biocompatible bioadhesives that perform underwater	1935:1992	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	7	3	theme	view	1133:1136	arg1	point					1124:1128	a composition point	1110:1128	a composition point of view	1110:1136	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	9	4	dep	structure	1575:1583	arg1	the					1571:1573	the	1571:1573	the	1571:1573	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	4	5	with	microscopy	572:581	arg1	microscopy					619:628	superresolution microscopy	603:628	superresolution microscopy	603:628	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	4	5	with	microscopy	572:581	arg1	assays					644:649	enzymatic assays	634:649	enzymatic assays	634:649	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	5	6	theme	core	894:897	arg1	layer					899:903	a stiffer nanoscopic core layer	873:903	two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer	861:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	4	7	used	used	554:557	arg2	we					551:552	we	551:552	we	551:552	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	10	8	theme	bioadhesive	1770:1780	arg1	mechanochemistry					1745:1760	the mechanochemistry	1741:1760	the mechanochemistry of this bioadhesive	1741:1780	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	14	9	theme	distinctive	2604:2614	arg1	properties					2625:2634	the distinctive adhesive properties	2600:2634	the distinctive adhesive properties of the holdfast	2600:2650	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	10	10	from	improvements	1708:1719	arg1	understanding					1724:1736	understanding	1724:1736	understanding of the mechanochemistry of this bioadhesive	1724:1780	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	13	11	theme	adhesive	2444:2451	arg1	strength					2453:2460	adhesive strength	2444:2460	adhesive strength	2444:2460	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	9	12	theme	distinctive	1676:1686	arg1	strength					1688:1695	its distinctive strength	1672:1695	its distinctive strength	1672:1695	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	8	13	theme	brush	1422:1426	arg1	layer					1428:1432	the brush layer	1418:1432	the brush layer	1418:1432	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	5	14	theme	stiffer	875:881	arg1	layer					899:903	a stiffer nanoscopic core layer	873:903	two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer	861:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	14	15	theme	diverse	2558:2564	arg1	chemistry					2566:2574	diverse chemistry	2558:2574	diverse chemistry	2558:2574	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	5	16	theme	brush	951:955	arg1	layer					957:961	a sparse, far-reaching, flexible brush layer	918:961	a sparse, far-reaching, flexible brush layer	918:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	13	17	from	peptides	2432:2439	arg1	strength					2453:2460	adhesive strength	2444:2460	adhesive strength	2444:2460	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	13	17	from	peptides	2432:2439	arg1	structure					2377:2385	the structure	2373:2385	the structure of the brush layer	2373:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	8	18	theme	initial	1454:1460	arg1	adhesion					1462:1469	initial adhesion	1454:1469	initial adhesion	1454:1469	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	14	19	theme	applications	2746:2757	arg1	mechanisms					2697:2706	the mechanisms	2693:2706	the mechanisms of bacterial adhesion and bioadhesive applications	2693:2757	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	4	20	theme	chemical	724:731	arg1	constituents					733:744	its chemical constituents	720:744	its chemical constituents	720:744	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	1	21	theme	aqueous	158:164	arg1	environments					166:177	aqueous environments	158:177	aqueous environments	158:177	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	6	22	theme	holdfast	1016:1023	arg1	response					1000:1007	the elastic response	988:1007	the elastic response of the holdfast	988:1023	Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous.
29437925	12	23	theme	brush	2271:2275	arg1	layer					2277:2281	a polymeric brush layer	2259:2281	a polymeric brush layer	2259:2281	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	4	24	dep	used	554:557	arg1	unravel					654:660	unravel	654:660	to unravel the complex structure of the holdfast	651:698	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	4	24	dep	used	554:557	arg1	characterize					707:718	characterize	707:718	to characterize its chemical constituents and their role in adhesion	704:771	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	8	25	theme	adhesive	1367:1374	arg1	force					1376:1380	adhesive force	1367:1380	adhesive force	1367:1380	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	7	26	contain	contains	1262:1269	arg2	DNA					1284:1286	DNA	1284:1286	DNA	1284:1286	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	7	26	contain	contains	1262:1269	arg2	peptides					1271:1278	peptides	1271:1278	peptides	1271:1278	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	7	26	contain	contains	1262:1269	arg1	holdfast					1253:1260	the holdfast	1249:1260	the holdfast	1249:1260	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	13	27	theme	layer	2400:2404	arg1	structure					2377:2385	the structure	2373:2385	the structure of the brush layer	2373:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	6	28	theme	elastic	992:998	arg1	response					1000:1007	the elastic response	988:1007	the elastic response of the holdfast	988:1023	Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous.
29437925	6	29	dep	heterogeneous	1070:1082	arg1	homogeneous					1092:1102	homogeneous	1092:1102	homogeneous	1092:1102	Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous.
29437925	6	29	dep	heterogeneous	1070:1082	arg1	to					1084:1085	to	1084:1085	to	1084:1085	Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous.
29437925	13	30	theme	initial	2410:2416	arg1	adhesion					2418:2425	initial adhesion	2410:2425	initial adhesion	2410:2425	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	14	31	theme	adhesion	2721:2728	arg1	mechanisms					2697:2706	the mechanisms	2693:2706	the mechanisms of bacterial adhesion and bioadhesive applications	2693:2757	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	8	32	theme	structural	1501:1510	arg1	role					1512:1515	a primarily structural role	1489:1515	a primarily structural role	1489:1515	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	5	33	dep	sparse	920:925	arg1	flexible					942:949	flexible	942:949	flexible	942:949	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	5	33	dep	sparse	920:925	arg1	far-reaching					928:939	far-reaching	928:939	far-reaching	928:939	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	0	34	theme	Layered	0:6	arg1	Structure					8:16	Layered Structure	0:16	Layered Structure	0:16	Layered Structure and Complex Mechanochemistry Underlie Strength and Versatility in a Bacterial Adhesive.
29437925	9	35	theme	unanticipated	1538:1550	arg1	complexity					1552:1561	The unanticipated complexity	1534:1561	The unanticipated complexity of both the structure and composition of the holdfast	1534:1615	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	4	36	from	microscopy	572:581	arg1	conjunction					586:596	conjunction	586:596	conjunction	586:596	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	12	37	theme	core	2244:2247	arg1	layer					2249:2253	a stiff core layer	2236:2253	a stiff core layer	2236:2253	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	14	38	theme	multilayer	2530:2539	arg1	organization					2541:2552	multilayer organization	2530:2552	multilayer organization	2530:2552	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	10	39	dep	adhesives.IMPORTANCE	1886:1905	arg1	is					1913:1914	is	1913:1914	is an urgent need for strong, biocompatible bioadhesives that perform underwater	1913:1992	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	3	40	theme	impressive	515:524	arg1	strength					535:542	impressive adhesive strength	515:542	impressive adhesive strength	515:542	The holdfast is extremely versatile and has impressive adhesive strength.
29437925	5	41	theme	heterogeneous	825:837	arg1	holdfast					811:818	the holdfast	807:818	the holdfast	807:818	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	5	41	theme	heterogeneous	825:837	arg1	material					839:846	a heterogeneous material	823:846	a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer	823:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	4	42	theme	atomic	559:564	arg1	microscopy					572:581	atomic force microscopy	559:581	atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays	559:649	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	5	43	dep	layers	865:870	arg1	layer					899:903	a stiffer nanoscopic core layer	873:903	two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer	861:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	9	44	theme	holdfast	1608:1615	arg1	structure					1575:1583	structure	1575:1583	structure	1575:1583	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	9	44	theme	holdfast	1608:1615	arg1	composition					1589:1599	composition	1589:1599	composition	1589:1599	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	11	45	dep	bacterium	2058:2066	arg1	crescentus					2080:2089	Caulobacter crescentus	2068:2089	the bacterium Caulobacter crescentus	2054:2089	To strongly adhere to surfaces and resist flow underwater, the bacterium Caulobacter crescentus produces an adhesive called the holdfast, the mechanochemistry of which remains undefined.
29437925	10	46	theme	Continued	1698:1706	arg1	improvements					1708:1719	Continued improvements	1698:1719	Continued improvements in understanding of the mechanochemistry of this bioadhesive	1698:1780	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	4	47	from	constituents	733:744	arg1	adhesion					764:771	adhesion	764:771	adhesion	764:771	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	4	48	theme	superresolution	603:617	arg1	microscopy					619:628	superresolution microscopy	603:628	superresolution microscopy	603:628	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	10	49	theme	adhesives.IMPORTANCE	1886:1905	arg1	development					1867:1877	the development	1863:1877	the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater	1863:1992	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	10	50	theme	mechanochemistry	1745:1760	arg1	understanding					1724:1736	understanding	1724:1736	understanding of the mechanochemistry of this bioadhesive	1724:1780	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	1	51	theme	substrates	288:297	arg1	surfaces					314:321	wet surfaces	310:321	wet surfaces	310:321	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	1	51	theme	substrates	288:297	arg1	substrates					288:297	substrates	288:297	substrates	288:297	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	1	51	theme	substrates	288:297	arg1	variety					277:283	a variety	275:283	a variety	275:283	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	2	52	theme	discrete	376:383	arg1	complex					400:406	a discrete polysaccharide complex	374:406	a discrete polysaccharide complex	374:406	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	2	52	theme	discrete	376:383	arg1	holdfast					413:420	the holdfast	409:420	the holdfast	409:420	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	7	53	theme	composition	1112:1122	arg1	point					1124:1128	a composition point	1110:1128	a composition point of view	1110:1136	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	5	54	theme	nanoscopic	883:892	arg1	layer					899:903	a stiffer nanoscopic core layer	873:903	two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer	861:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	4	55	theme	holdfast	691:698	arg1	structure					674:682	the complex structure	662:682	the complex structure of the holdfast	662:698	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	10	56	attach	attach	1827:1832	arg2	bacteria					1818:1825	bacteria	1818:1825	bacteria	1818:1825	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	10	56	attach	attach	1827:1832	arg1	surfaces					1837:1844	surfaces	1837:1844	surfaces	1837:1844	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	10	57	theme	urgent	1919:1924	arg1	need					1926:1929	an urgent need	1916:1929	an urgent need for strong, biocompatible bioadhesives that perform underwater	1916:1992	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	14	58	theme	adhesive	2616:2623	arg1	properties					2625:2634	the distinctive adhesive properties	2600:2634	the distinctive adhesive properties of the holdfast	2600:2650	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	11	59	theme	flow	2037:2040	arg1	underwater					2042:2051	flow underwater	2037:2051	flow underwater	2037:2051	To strongly adhere to surfaces and resist flow underwater, the bacterium Caulobacter crescentus produces an adhesive called the holdfast, the mechanochemistry of which remains undefined.
29437925	13	60	from	polysaccharides	2471:2485	arg1	strength					2453:2460	adhesive strength	2444:2460	adhesive strength	2444:2460	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	13	60	from	polysaccharides	2471:2485	arg1	structure					2377:2385	the structure	2373:2385	the structure of the brush layer	2373:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	2	61	theme	aquatic	328:334	arg1	bacterium					336:344	The aquatic bacterium	324:344	The aquatic bacterium Caulobacter crescentus	324:367	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	4	62	theme	complex	666:672	arg1	structure					674:682	the complex structure	662:682	the complex structure of the holdfast	662:698	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	1	63	attach	attach	265:270	arg1	surfaces					314:321	wet surfaces	310:321	wet surfaces	310:321	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	1	63	attach	attach	265:270	arg2	bioadhesives					235:246	bioadhesives	235:246	bioadhesives that efficiently attach to a variety of substrates, including wet surfaces	235:321	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	1	63	attach	attach	265:270	arg1	substrates					288:297	substrates	288:297	substrates	288:297	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	1	63	attach	attach	265:270	arg1	variety					277:283	a variety	275:283	a variety	275:283	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	10	64	theme	new	1796:1798	arg1	insights					1800:1807	new insights	1796:1807	new insights into how bacteria attach to surfaces	1796:1844	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	1	65	theme	synthetic	122:130	arg1	adhesives					132:140	synthetic adhesives	122:140	synthetic adhesives that perform in aqueous environments	122:177	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	11	66	theme	adhesive	2103:2110	arg1	mechanochemistry					2137:2152	the mechanochemistry	2133:2152	the mechanochemistry	2133:2152	To strongly adhere to surfaces and resist flow underwater, the bacterium Caulobacter crescentus produces an adhesive called the holdfast, the mechanochemistry of which remains undefined.
29437925	6	67	theme	surface	1039:1045	arg1	contact					1047:1053	surface contact	1039:1053	surface contact	1039:1053	Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous.
29437925	8	68	theme	adhesion	1462:1469	arg1	layer					1428:1432	the brush layer	1418:1432	the brush layer	1418:1432	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	8	68	theme	adhesion	1462:1469	arg1	strength					1442:1449	the strength	1438:1449	the strength of initial adhesion	1438:1469	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	2	69	dep	bacterium	336:344	arg1	crescentus					358:367	Caulobacter crescentus	346:367	The aquatic bacterium Caulobacter crescentus	324:367	The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow.
29437925	13	70	from	adhesion	2418:2425	arg1	strength					2453:2460	adhesive strength	2444:2460	adhesive strength	2444:2460	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	13	70	from	adhesion	2418:2425	arg1	structure					2377:2385	the structure	2373:2385	the structure of the brush layer	2373:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	14	71	theme	bioadhesive	2734:2744	arg1	applications					2746:2757	bioadhesive applications	2734:2757	bioadhesive applications	2734:2757	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	12	72	with	structure	2221:2229	arg1	layer					2277:2281	a polymeric brush layer	2259:2281	a polymeric brush layer	2259:2281	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	12	72	with	structure	2221:2229	arg1	layer					2249:2253	a stiff core layer	2236:2253	a stiff core layer	2236:2253	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	5	73	theme	sparse	920:925	arg1	layer					957:961	a sparse, far-reaching, flexible brush layer	918:961	a sparse, far-reaching, flexible brush layer	918:961	Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer.
29437925	13	74	theme	brush	2394:2398	arg1	layer					2400:2404	the brush layer	2390:2404	the brush layer	2390:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	3	75	contain	has	511:513	arg2	strength					535:542	impressive adhesive strength	515:542	impressive adhesive strength	515:542	The holdfast is extremely versatile and has impressive adhesive strength.
29437925	3	75	contain	has	511:513	arg1	holdfast					475:482	The holdfast	471:482	The holdfast	471:482	The holdfast is extremely versatile and has impressive adhesive strength.
29437925	3	75	contain	has	511:513	arg1	versatile					497:505	versatile	497:505	versatile	497:505	The holdfast is extremely versatile and has impressive adhesive strength.
29437925	12	76	theme	polymeric	2261:2269	arg1	layer					2277:2281	a polymeric brush layer	2259:2281	a polymeric brush layer	2259:2281	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	12	77	theme	stiff	2238:2242	arg1	layer					2249:2253	a stiff core layer	2236:2253	a stiff core layer	2236:2253	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	7	78	theme	only	1181:1184	arg1	N-acetyl-d-glucosamine					1147:1168	N-acetyl-d-glucosamine	1147:1168	N-acetyl-d-glucosamine (NAG)	1147:1174	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	7	78	theme	only	1181:1184	arg1	component					1186:1194	the only component	1177:1194	the only component that had been identified to date	1177:1227	From a composition point of view, besides N-acetyl-d-glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA.
29437925	14	79	theme	bacterial	2711:2719	arg1	adhesion					2721:2728	bacterial adhesion	2711:2728	bacterial adhesion	2711:2728	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	13	80	from	role	2365:2368	arg1	strength					2453:2460	adhesive strength	2444:2460	adhesive strength	2444:2460	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	13	80	from	role	2365:2368	arg1	structure					2377:2385	the structure	2373:2385	the structure of the brush layer	2373:2404	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	0	81	theme	Complex	22:28	arg1	Mechanochemistry					30:45	Complex Mechanochemistry	22:45	Complex Mechanochemistry	22:45	Layered Structure and Complex Mechanochemistry Underlie Strength and Versatility in a Bacterial Adhesive.
29437925	1	82	theme	wet	310:312	arg1	surfaces					314:321	wet surfaces	310:321	wet surfaces	310:321	While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces.
29437925	10	83	dep	strong	1935:1940	arg1	biocompatible					1943:1955	biocompatible	1943:1955	biocompatible	1943:1955	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	8	84	theme	important	1342:1350	arg1	components					1352:1361	the most important components	1333:1361	the most important components for adhesive force	1333:1380	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	8	84	theme	important	1342:1350	arg1	polypeptides					1316:1327	polypeptides	1316:1327	polypeptides	1316:1327	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	12	85	theme	layered	2213:2219	arg1	structure					2221:2229	a layered structure	2211:2229	a layered structure with a stiff core layer and a polymeric brush layer	2211:2281	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	12	85	theme	layered	2213:2219	arg1	holdfast					2199:2206	the holdfast	2195:2206	the holdfast	2195:2206	We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA.
29437925	13	86	theme	core	2511:2514	arg1	structure					2494:2502	the structure	2490:2502	the structure of the core	2490:2514	The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core.
29437925	9	87	theme	structure	1575:1583	arg1	complexity					1552:1561	The unanticipated complexity	1534:1561	The unanticipated complexity of both the structure and composition of the holdfast	1534:1615	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	14	88	theme	important	2669:2677	arg1	insights					2679:2686	important insights	2669:2686	important insights into the mechanisms of bacterial adhesion and bioadhesive applications	2669:2757	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
29437925	4	89	theme	force	566:570	arg1	microscopy					572:581	atomic force microscopy	559:581	atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays	559:649	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	10	90	theme	new	1882:1884	arg1	adhesives.IMPORTANCE					1886:1905	new adhesives.IMPORTANCE	1882:1905	new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater	1882:1992	Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives.IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater.
29437925	3	91	theme	adhesive	526:533	arg1	strength					535:542	impressive adhesive strength	515:542	impressive adhesive strength	515:542	The holdfast is extremely versatile and has impressive adhesive strength.
29437925	9	92	theme	composition	1589:1599	arg1	complexity					1552:1561	The unanticipated complexity	1534:1561	The unanticipated complexity of both the structure and composition of the holdfast	1534:1615	The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength.
29437925	8	93	theme	DNA	1399:1401	arg1	presence					1387:1394	the presence	1383:1394	the presence of DNA	1383:1401	We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core.
29437925	4	94	from	role	756:759	arg1	adhesion					764:771	adhesion	764:771	adhesion	764:771	Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion.
29437925	14	95	theme	holdfast	2643:2650	arg1	properties					2625:2634	the distinctive adhesive properties	2600:2634	the distinctive adhesive properties of the holdfast	2600:2650	The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications.
31313800	0	0	theme	cyclodextrin	86:97	arg1	scaffolds					99:107	cyclodextrin scaffolds	86:107	cyclodextrin scaffolds	86:107	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters based on cyclodextrin scaffolds.
31313800	3	1	theme	type	324:327	arg1	diabetes					331:338	type 2 diabetes	324:338	type 2 diabetes	324:338	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
31313800	3	1	theme	type	324:327	arg1	kind					367:370	the non-insulin dependent kind	341:370	the non-insulin dependent kind	341:370	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
31313800	8	2	theme	non-protected	1182:1194	arg1	monosaccharides					1210:1224	various protected and non-protected propargylated monosaccharides	1160:1224	various protected and non-protected propargylated monosaccharides	1160:1224	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	2	theme	non-protected	1182:1194	arg1	S-glycosides					1249:1260	S-glycosides	1249:1260	S-glycosides	1249:1260	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	2	theme	non-protected	1182:1194	arg1	O-					1242:1243	O-	1242:1243	O-	1242:1243	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	4	3	theme	competitive	499:509	arg1	inhibitors					511:520	competitive inhibitors	499:520	competitive inhibitors	499:520	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	4	3	theme	competitive	499:509	arg1	iminosugars					531:541	iminosugars	531:541	iminosugars (e.g. acarbose)	531:557	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	4	3	theme	competitive	499:509	arg1	derivatives					578:588	sulfonium sugar derivatives	562:588	sulfonium sugar derivatives (e.g. salacinol)	562:605	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	6	4	theme	α-d-glucosidase	938:952	arg1	inhibitors					924:933	inhibitors	924:933	inhibitors of α-d-glucosidase	924:952	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	10	5	theme	μM	1534:1535	arg1	range					1537:1541	the 32-132 μM range	1523:1541	the 32-132 μM range	1523:1541	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	1	6	theme	dramatic	169:176	arg1	worldwide					194:202	a dramatic chronic disease worldwide	167:202	a dramatic chronic disease worldwide	167:202	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	1	6	theme	dramatic	169:176	arg1	diabetes					157:164	diabetes	157:164	diabetes	157:164	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	4	7	theme	sugar	572:576	arg1	derivatives					578:588	sulfonium sugar derivatives	562:588	sulfonium sugar derivatives (e.g. salacinol)	562:605	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	3	8	theme	dependent	357:365	arg1	diabetes					331:338	type 2 diabetes	324:338	type 2 diabetes	324:338	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
31313800	3	8	theme	dependent	357:365	arg1	kind					367:370	the non-insulin dependent kind	341:370	the non-insulin dependent kind	341:370	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
31313800	10	9	theme	=	1578:1578	arg1	μM					1585:1586	IC50 = ∼250 μM	1573:1586	IC50 = ∼250 μM	1573:1586	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	9	theme	=	1578:1578	arg1	acarbose					1563:1570	acarbose	1563:1570	acarbose (IC50 = ∼250 μM)	1563:1587	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	5	10	theme	multivalent	654:664	arg1	nanoparticles					674:686	multivalent diamond nanoparticles	654:686	multivalent diamond nanoparticles grafted with unmodified sugars	654:717	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	2	11	theme	insulin	293:299	arg1	treatment					301:309	insulin treatment	293:309	insulin treatment	293:309	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	5	12	theme	low	757:759	arg1	concentrations					772:785	low micromolar concentrations	757:785	low micromolar concentrations	757:785	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	5	13	theme	diamond	666:672	arg1	nanoparticles					674:686	multivalent diamond nanoparticles	654:686	multivalent diamond nanoparticles grafted with unmodified sugars	654:717	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	5	14	theme	micromolar	761:770	arg1	concentrations					772:785	low micromolar concentrations	757:785	low micromolar concentrations	757:785	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	6	15	theme	glycoclusters	836:848	arg1	synthesis					811:819	the synthesis	807:819	the synthesis of multivalent glycoclusters	807:848	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	1	16	theme	chronic	178:184	arg1	worldwide					194:202	a dramatic chronic disease worldwide	167:202	a dramatic chronic disease worldwide	167:202	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	1	16	theme	chronic	178:184	arg1	diabetes					157:164	diabetes	157:164	diabetes	157:164	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	3	17	from	problematic	376:386	arg1	contrast					315:322	contrast	315:322	contrast	315:322	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
31313800	8	18	theme	versatility	1292:1302	arg1	evidence					1276:1283	clear evidence	1270:1283	clear evidence of its versatility	1270:1302	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	6	19	theme	multivalent	824:834	arg1	glycoclusters					836:848	multivalent glycoclusters	824:848	multivalent glycoclusters	824:848	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	1	20	theme	disease	186:192	arg1	worldwide					194:202	a dramatic chronic disease worldwide	167:202	a dramatic chronic disease worldwide	167:202	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	1	20	theme	disease	186:192	arg1	diabetes					157:164	diabetes	157:164	diabetes	157:164	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	0	21	theme	uncommon	4:11	arg1	inhibition					20:29	The uncommon strong inhibition	0:29	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters	0:75	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters based on cyclodextrin scaffolds.
31313800	4	22	dep	acarbose	549:556	arg1	e.g.					544:547	e.g.	544:547	e.g.	544:547	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	5	23	theme	unprecedented	621:633	arg1	result					635:640	an unprecedented result	618:640	an unprecedented result	618:640	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	8	24	theme	various	1160:1166	arg1	monosaccharides					1210:1224	various protected and non-protected propargylated monosaccharides	1160:1224	various protected and non-protected propargylated monosaccharides	1160:1224	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	24	theme	various	1160:1166	arg1	S-glycosides					1249:1260	S-glycosides	1249:1260	S-glycosides	1249:1260	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	24	theme	various	1160:1166	arg1	O-					1242:1243	O-	1242:1243	O-	1242:1243	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	9	25	theme	gel	1406:1408	arg1	chromatography					1410:1423	silica gel chromatography	1399:1423	silica gel chromatography	1399:1423	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	4	26	theme	level	424:428	arg1	control					393:399	The control	389:399	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation	389:472	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	10	27	theme	IC50	1508:1511	arg1	values					1513:1518	IC50 values	1508:1518	IC50 values in the 32-132 μM range	1508:1541	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	9	28	theme	6-per-glycosylated	1318:1335	arg1	CDs					1337:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	10	29	from	values	1513:1518	arg1	range					1537:1541	the 32-132 μM range	1523:1541	the 32-132 μM range	1523:1541	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	11	30	theme	inhibition	1737:1746	arg1	mode					1748:1751	a mixed-type non-competitive inhibition mode	1708:1751	a mixed-type non-competitive inhibition mode for these new glycoclusters	1708:1779	Preliminary experiments suggest a mixed-type non-competitive inhibition mode for these new glycoclusters.
31313800	10	31	theme	IC50	1573:1576	arg1	μM					1585:1586	IC50 = ∼250 μM	1573:1586	IC50 = ∼250 μM	1573:1586	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	31	theme	IC50	1573:1576	arg1	acarbose					1563:1570	acarbose	1563:1570	acarbose (IC50 = ∼250 μM)	1563:1587	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	7	32	theme	per-azido	1004:1012	arg1	α					1014:1014	per-azido α	1004:1014	per-azido α	1004:1014	The glycoclusters were efficiently obtained from per-azido α, β and γ-CD derivatives and propargyl glycosides using click-chemistry under microwave irradiation.
31313800	0	33	theme	strong	13:18	arg1	inhibition					20:29	The uncommon strong inhibition	0:29	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters	0:75	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters based on cyclodextrin scaffolds.
31313800	4	34	theme	sulfonium	562:570	arg1	derivatives					578:588	sulfonium sugar derivatives	562:588	sulfonium sugar derivatives (e.g. salacinol)	562:605	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	4	35	theme	α-d-glucosidase	445:459	arg1	inactivation					461:472	intestinal α-d-glucosidase inactivation	434:472	intestinal α-d-glucosidase inactivation	434:472	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	1	36	theme	homeostasis	114:124	arg1	disruption					126:135	The homeostasis disruption	110:135	The homeostasis disruption of d-glucose	110:148	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	0	37	theme	α-glucosidase	34:46	arg1	inhibition					20:29	The uncommon strong inhibition	0:29	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters	0:75	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters based on cyclodextrin scaffolds.
31313800	9	38	dep	yields	1380:1385	arg1	to					1367:1368	to	1367:1368	to	1367:1368	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	5	39	theme	α-glucosidase	729:741	arg1	inhibition					743:752	α-glucosidase inhibition	729:752	α-glucosidase inhibition	729:752	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	4	40	theme	intestinal	434:443	arg1	inactivation					461:472	intestinal α-d-glucosidase inactivation	434:472	intestinal α-d-glucosidase inactivation	434:472	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	4	41	dep	salacinol	596:604	arg1	e.g.					591:594	e.g.	591:594	e.g.	591:594	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	9	42	gly	6-per-glycosylated	1318:1335	arg1	CDs					1337:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	2	43	theme	treatable	239:247	arg1	form					249:252	a successfully treatable form	224:252	a successfully treatable form	224:252	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	2	43	theme	treatable	239:247	arg1	diabetes					212:219	Type 1 diabetes	205:219	Type 1 diabetes	205:219	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	10	44	from	inhibition	1445:1454	arg1	cerevisiae					1492:1501	Saccharomyces cerevisiae	1478:1501	Saccharomyces cerevisiae	1478:1501	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	0	45	theme	multivalent	51:61	arg1	glycoclusters					63:75	multivalent glycoclusters	51:75	multivalent glycoclusters	51:75	The uncommon strong inhibition of α-glucosidase by multivalent glycoclusters based on cyclodextrin scaffolds.
31313800	7	46	theme	microwave	1093:1101	arg1	irradiation					1103:1113	microwave irradiation	1093:1113	microwave irradiation	1093:1113	The glycoclusters were efficiently obtained from per-azido α, β and γ-CD derivatives and propargyl glycosides using click-chemistry under microwave irradiation.
31313800	10	47	theme	well-known	1592:1601	arg1	inhibitor					1615:1623	a well-known competitive inhibitor	1590:1623	a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes	1590:1673	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	47	theme	well-known	1592:1601	arg1	that					1555:1558	that	1555:1558	that	1555:1558	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	6	48	theme	role	916:919	arg1	cyclodextrins					856:868	cyclodextrins	856:868	cyclodextrins (CDs)	856:874	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	6	48	theme	role	916:919	arg1	scaffolds					879:887	scaffolds	879:887	scaffolds	879:887	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	6	48	theme	role	916:919	arg1	assessment					896:905	an assessment	893:905	an assessment of their role as inhibitors of α-d-glucosidase	893:952	Herein we describe the synthesis of multivalent glycoclusters using cyclodextrins (CDs) as scaffolds and an assessment of their role as inhibitors of α-d-glucosidase.
31313800	8	49	theme	protected	1168:1176	arg1	monosaccharides					1210:1224	various protected and non-protected propargylated monosaccharides	1160:1224	various protected and non-protected propargylated monosaccharides	1160:1224	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	49	theme	protected	1168:1176	arg1	S-glycosides					1249:1260	S-glycosides	1249:1260	S-glycosides	1249:1260	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	49	theme	protected	1168:1176	arg1	O-					1242:1243	O-	1242:1243	O-	1242:1243	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	4	50	theme	blood	418:422	arg1	level					424:428	the d-glucose blood level	404:428	the d-glucose blood level	404:428	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	10	51	theme	32-132	1527:1532	arg1	μM					1534:1535	μM	1534:1535	μM	1534:1535	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	52	theme	clinical	1637:1644	arg1	treatment					1646:1654	the clinical treatment	1633:1654	the clinical treatment of type 2 diabetes	1633:1673	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	8	53	theme	propargylated	1196:1208	arg1	monosaccharides					1210:1224	various protected and non-protected propargylated monosaccharides	1160:1224	various protected and non-protected propargylated monosaccharides	1160:1224	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	53	theme	propargylated	1196:1208	arg1	S-glycosides					1249:1260	S-glycosides	1249:1260	S-glycosides	1249:1260	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	8	53	theme	propargylated	1196:1208	arg1	O-					1242:1243	O-	1242:1243	O-	1242:1243	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	4	54	theme	d-glucose	408:416	arg1	level					424:428	the d-glucose blood level	404:428	the d-glucose blood level	404:428	The control of the d-glucose blood level via intestinal α-d-glucosidase inactivation can be achieved by using competitive inhibitors, such as iminosugars (e.g. acarbose) or sulfonium sugar derivatives (e.g. salacinol).
31313800	7	55	theme	propargyl	1044:1052	arg1	glycosides					1054:1063	propargyl glycosides	1044:1063	propargyl glycosides using click-chemistry under microwave irradiation	1044:1113	The glycoclusters were efficiently obtained from per-azido α, β and γ-CD derivatives and propargyl glycosides using click-chemistry under microwave irradiation.
31313800	1	56	theme	d-glucose	140:148	arg1	disruption					126:135	The homeostasis disruption	110:135	The homeostasis disruption of d-glucose	110:148	The homeostasis disruption of d-glucose causes diabetes, a dramatic chronic disease worldwide.
31313800	11	57	theme	non-competitive	1721:1735	arg1	mode					1748:1751	a mixed-type non-competitive inhibition mode	1708:1751	a mixed-type non-competitive inhibition mode for these new glycoclusters	1708:1779	Preliminary experiments suggest a mixed-type non-competitive inhibition mode for these new glycoclusters.
31313800	5	58	theme	unmodified	701:710	arg1	sugars					712:717	unmodified sugars	701:717	unmodified sugars	701:717	Recently, an unprecedented result showed that multivalent diamond nanoparticles grafted with unmodified sugars displayed α-glucosidase inhibition at low micromolar concentrations.
31313800	9	59	theme	moderate	1358:1365	arg1	yields					1380:1385	moderate to excellent yields	1358:1385	moderate to excellent yields (30-90%)	1358:1394	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	9	59	theme	moderate	1358:1365	arg1	%					1393:1393	30-90%	1388:1393	30-90%	1388:1393	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	2	60	theme	blood	261:265	arg1	d-glucose					267:275	blood d-glucose	261:275	blood d-glucose	261:275	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	7	61	theme	γ-CD	1023:1026	arg1	derivatives					1028:1038	β and γ-CD derivatives	1017:1038	derivatives	1028:1038	The glycoclusters were efficiently obtained from per-azido α, β and γ-CD derivatives and propargyl glycosides using click-chemistry under microwave irradiation.
31313800	9	62	theme	silica	1399:1404	arg1	chromatography					1410:1423	silica gel chromatography	1399:1423	silica gel chromatography	1399:1423	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	11	63	theme	mixed-type	1710:1719	arg1	mode					1748:1751	a mixed-type non-competitive inhibition mode	1708:1751	a mixed-type non-competitive inhibition mode for these new glycoclusters	1708:1779	Preliminary experiments suggest a mixed-type non-competitive inhibition mode for these new glycoclusters.
31313800	10	64	from	cerevisiae	1492:1501	arg1	α-glucosidase					1459:1471	α-glucosidase	1459:1471	α-glucosidase from Saccharomyces cerevisiae	1459:1501	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	64	from	cerevisiae	1492:1501	arg1	inhibition					1445:1454	inhibition	1445:1454	inhibition of α-glucosidase from Saccharomyces cerevisiae	1445:1501	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	65	theme	competitive	1603:1613	arg1	inhibitor					1615:1623	a well-known competitive inhibitor	1590:1623	a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes	1590:1673	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	65	theme	competitive	1603:1613	arg1	that					1555:1558	that	1555:1558	that	1555:1558	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	2	66	theme	Type	205:208	arg1	form					249:252	a successfully treatable form	224:252	a successfully treatable form	224:252	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	2	66	theme	Type	205:208	arg1	diabetes					212:219	Type 1 diabetes	205:219	Type 1 diabetes	205:219	Type 1 diabetes is a successfully treatable form, where blood d-glucose is regulated by insulin treatment.
31313800	9	67	theme	targeted	1309:1316	arg1	CDs					1337:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs	1305:1339	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	11	68	theme	Preliminary	1676:1686	arg1	experiments					1688:1698	Preliminary experiments	1676:1698	Preliminary experiments	1676:1698	Preliminary experiments suggest a mixed-type non-competitive inhibition mode for these new glycoclusters.
31313800	10	69	theme	α-glucosidase	1459:1471	arg1	inhibition					1445:1454	inhibition	1445:1454	inhibition of α-glucosidase from Saccharomyces cerevisiae	1445:1501	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	10	70	theme	diabetes	1666:1673	arg1	treatment					1646:1654	the clinical treatment	1633:1654	the clinical treatment of type 2 diabetes	1633:1673	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	9	71	theme	excellent	1370:1378	arg1	yields					1380:1385	moderate to excellent yields	1358:1385	moderate to excellent yields (30-90%)	1358:1394	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	9	71	theme	excellent	1370:1378	arg1	%					1393:1393	30-90%	1388:1393	30-90%	1388:1393	The targeted 6-per-glycosylated CDs were isolated in moderate to excellent yields (30-90%) by silica gel chromatography.
31313800	10	72	theme	type	1659:1662	arg1	diabetes					1666:1673	type 2 diabetes	1659:1673	type 2 diabetes	1659:1673	The results showed inhibition of α-glucosidase from Saccharomyces cerevisiae with IC50 values in the 32-132 μM range, lower than that of acarbose (IC50 = ∼250 μM), a well-known competitive inhibitor used in the clinical treatment of type 2 diabetes.
31313800	7	73	theme	β	1017:1017	arg1	derivatives					1028:1038	β and γ-CD derivatives	1017:1038	derivatives	1028:1038	The glycoclusters were efficiently obtained from per-azido α, β and γ-CD derivatives and propargyl glycosides using click-chemistry under microwave irradiation.
31313800	8	74	theme	clear	1270:1274	arg1	evidence					1276:1283	clear evidence	1270:1283	clear evidence of its versatility	1270:1302	The methodology was successfully applied to various protected and non-protected propargylated monosaccharides, including both O- and S-glycosides, giving clear evidence of its versatility.
31313800	11	75	theme	new	1763:1765	arg1	glycoclusters					1767:1779	these new glycoclusters	1757:1779	these new glycoclusters	1757:1779	Preliminary experiments suggest a mixed-type non-competitive inhibition mode for these new glycoclusters.
31313800	3	76	from	contrast	315:322	arg1	problematic					376:386	problematic	376:386	problematic	376:386	In contrast type 2 diabetes, the non-insulin dependent kind, is problematic.
30696937	1	0	theme	B	240:240	arg1	α1,3-galactosyltransferase					350:375	α1,3-galactosyltransferase	350:375	α1,3-galactosyltransferase (GT)	350:380	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	synthase					288:295	Forssman glycolipid synthase	268:295	Forssman glycolipid synthase (FS)	268:300	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	synthase					328:335	isoglobotriaosylceramide synthase	303:335	isoglobotriaosylceramide synthase (iGb3S)	303:343	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	AT					256:257	AT	256:257	AT	256:257	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	BT					263:264	BT	263:264	BT	263:264	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	0	theme	B	240:240	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	11	1	theme	transferases	2073:2084	arg1	generation					2045:2054	the generation	2041:2054	the generation of α1,3-Gal(NAc) transferases with diversified specificities	2041:2115	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	7	2	theme	human	1404:1408	arg1	AT/BT					1410:1414	human AT/BT	1404:1414	human AT/BT	1404:1414	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	3	theme	FS-specific	1303:1313	arg1	tripeptides					1229:1239	selected tripeptides	1220:1239	selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly	1220:1277	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	3	theme	FS-specific	1303:1313	arg1	GlyGlyAla					1315:1323	FS-specific GlyGlyAla	1303:1323	FS-specific GlyGlyAla	1303:1323	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	0	4	with	transferases	105:116	arg1	activity					146:153	differential enzymatic activity	123:153	differential enzymatic activity	123:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	4	5	theme	AT/BT	646:650	arg1	codons					618:623	codons 266 and 268	618:635	codons	618:623	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	1	6	dep	transferases	242:253	arg1	BT					263:264	BT	263:264	BT	263:264	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	6	dep	transferases	242:253	arg1	AT					256:257	AT	256:257	AT	256:257	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	6	dep	transferases	242:253	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	11	7	theme	diversified	2091:2101	arg1	specificities					2103:2115	diversified specificities	2091:2115	diversified specificities	2091:2115	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	4	8	from	codons	618:623	arg1	crucial					656:662	crucial	656:662	crucial	656:662	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	8	from	codons	618:623	arg1	acids					609:613	the amino acids	599:613	the amino acids at codons 266 and 268 of human AT/BT	599:650	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	0	9	theme	enzymatic	136:144	arg1	activity					146:153	differential enzymatic activity	123:153	differential enzymatic activity	123:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	4	10	theme	sugar	682:686	arg1	specificities					688:700	their distinct sugar specificities	667:700	their distinct sugar specificities	667:700	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	8	11	theme	appropriate	1454:1464	arg1	cells					1476:1480	appropriate recipient cells	1454:1480	appropriate recipient cells existing and newly created	1454:1507	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	7	12	theme	GT-specific	1339:1349	arg1	HisAlaAla					1351:1359	GT-specific HisAlaAla	1339:1359	GT-specific HisAlaAla	1339:1359	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	11	13	theme	α1,3-Gal	2059:2066	arg1	transferases					2073:2084	α1,3-Gal(NAc) transferases	2059:2084	α1,3-Gal(NAc) transferases	2059:2084	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	11	13	theme	α1,3-Gal	2059:2066	arg1	NAc					2068:2070	NAc	2068:2070	NAc	2068:2070	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	5	14	theme	amino	921:925	arg1	acids					927:931	20 possible amino acids	909:931	20 possible amino acids at those codons	909:947	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	5	15	contain	having	895:900	arg2	any					902:904	any	902:904	any	902:904	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	5	15	contain	having	895:900	arg1	ATs/BTs					887:893	in vitro mutagenized ATs/BTs	866:893	in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons	866:947	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	0	16	theme	blood	64:68	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	4	17	from	polymorphism	768:779	arg1	transfusion					807:817	transfusion	807:817	transfusion	807:817	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	17	from	polymorphism	768:779	arg1	medicine					839:846	transplantation medicine	823:846	transplantation medicine	823:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	1	18	theme	Functional	156:165	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	18	theme	Functional	156:165	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	18	theme	Functional	156:165	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	0	19	theme	system-related	76:89	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	1	20	theme	ABO	178:180	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	20	theme	ABO	178:180	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	20	theme	ABO	178:180	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	8	21	theme	created	1501:1507	arg1	cells					1476:1480	appropriate recipient cells	1454:1480	appropriate recipient cells existing and newly created	1454:1507	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	1	22	theme	isoglobotriaosylceramide	303:326	arg1	synthase					328:335	isoglobotriaosylceramide synthase	303:335	isoglobotriaosylceramide synthase (iGb3S)	303:343	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	22	theme	isoglobotriaosylceramide	303:326	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	22	theme	isoglobotriaosylceramide	303:326	arg1	iGb3S					338:342	iGb3S	338:342	iGb3S	338:342	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	3	23	theme	acceptor	533:540	arg1	substrates					542:551	their acceptor substrates	527:551	their acceptor substrates	527:551	However, their acceptor substrates are diverse.
30696937	2	24	theme	α1,3-glycosidic	493:507	arg1	linkage					509:515	an α1,3-glycosidic linkage	490:515	an α1,3-glycosidic linkage	490:515	These glycosyltransferases transfer N-acetyl-d-galactosamine (GalNAc) or d-galactose forming an α1,3-glycosidic linkage.
30696937	4	25	theme	ABO	750:752	arg1	polymorphism					768:779	the ABO glycosylation polymorphism	746:779	the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	746:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	11	26	theme	different	1958:1966	arg1	sequences					1979:1987	different tripeptide sequences	1958:1987	different tripeptide sequences at the substrate-binding pocket	1958:2019	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	4	27	from	medicine	839:846	arg1	basis					737:741	the molecular genetic basis	715:741	the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	715:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	0	28	theme	Amino	0:4	arg1	substitutions					11:23	Amino acid substitutions	0:23	Amino acid substitutions at sugar-recognizing codons	0:51	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	8	29	theme	cell	1532:1535	arg1	antigens					1561:1568	cell surface oligosaccharide antigens	1532:1568	cell surface oligosaccharide antigens	1532:1568	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	4	30	theme	genetic	729:735	arg1	basis					737:741	the molecular genetic basis	715:741	the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	715:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	8	31	theme	oligosaccharide	1545:1559	arg1	antigens					1561:1568	cell surface oligosaccharide antigens	1532:1568	cell surface oligosaccharide antigens	1532:1568	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	0	32	theme	sugar-recognizing	28:44	arg1	codons					46:51	sugar-recognizing codons	28:51	sugar-recognizing codons	28:51	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	7	33	theme	BT-specific	1280:1290	arg1	MetGlyAla					1292:1300	BT-specific MetGlyAla	1280:1300	BT-specific MetGlyAla	1280:1300	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	33	theme	BT-specific	1280:1290	arg1	tripeptides					1229:1239	selected tripeptides	1220:1239	selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly	1220:1277	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	10	34	theme	donor	1888:1892	arg1	nucleotide-sugar					1894:1909	donor nucleotide-sugar and acceptor substrates	1888:1933	nucleotide-sugar	1894:1909	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	7	35	theme	Eukaryotic	1136:1145	arg1	constructs					1158:1167	Eukaryotic expression constructs	1136:1167	Eukaryotic expression constructs	1136:1167	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	1	36	theme	glycolipid	277:286	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	36	theme	glycolipid	277:286	arg1	FS					298:299	FS	298:299	FS	298:299	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	36	theme	glycolipid	277:286	arg1	synthase					288:295	Forssman glycolipid synthase	268:295	Forssman glycolipid synthase (FS)	268:300	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	4	37	theme	transplantation	823:837	arg1	medicine					839:846	transplantation medicine	823:846	transplantation medicine	823:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	7	38	theme	LeuGlyGly	1269:1277	arg1	tripeptides					1229:1239	selected tripeptides	1220:1239	selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly	1220:1277	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	38	theme	LeuGlyGly	1269:1277	arg1	MetGlyAla					1292:1300	BT-specific MetGlyAla	1280:1300	BT-specific MetGlyAla	1280:1300	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	38	theme	LeuGlyGly	1269:1277	arg1	iGb3S					1329:1333	iGb3S	1329:1333	iGb3S	1329:1333	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	38	theme	LeuGlyGly	1269:1277	arg1	GlyGlyAla					1315:1323	FS-specific GlyGlyAla	1303:1323	FS-specific GlyGlyAla	1303:1323	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	4	39	theme	polymorphism	768:779	arg1	basis					737:741	the molecular genetic basis	715:741	the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	715:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	40	theme	importance	793:802	arg1	polymorphism					768:779	the ABO glycosylation polymorphism	746:779	the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	746:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	5	41	from	codons	942:947	arg1	acids					927:931	20 possible amino acids	909:931	20 possible amino acids at those codons	909:947	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	9	42	theme	several	1628:1634	arg1	tripeptides					1636:1646	several tripeptides	1628:1646	several tripeptides other than the originals	1628:1671	The results have shown that several tripeptides other than the originals also bestowed transferase activity.
30696937	7	43	theme	AlaGlyGly	1256:1264	arg1	tripeptides					1229:1239	selected tripeptides	1220:1239	selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly	1220:1277	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	43	theme	AlaGlyGly	1256:1264	arg1	MetGlyAla					1292:1300	BT-specific MetGlyAla	1280:1300	BT-specific MetGlyAla	1280:1300	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	43	theme	AlaGlyGly	1256:1264	arg1	iGb3S					1329:1333	iGb3S	1329:1333	iGb3S	1329:1333	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	43	theme	AlaGlyGly	1256:1264	arg1	GlyGlyAla					1315:1323	FS-specific GlyGlyAla	1303:1323	FS-specific GlyGlyAla	1303:1323	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	8	44	theme	DNA	1417:1419	arg1	transfection					1421:1432	DNA transfection	1417:1432	DNA transfection	1417:1432	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	10	45	theme	acids	1753:1757	arg1	repertoire					1722:1731	the repertoire	1718:1731	the repertoire of functional amino acids	1718:1757	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	10	45	theme	acids	1753:1757	arg1	acids					1753:1757	functional amino acids	1736:1757	functional amino acids	1736:1757	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	1	46	theme	group	228:232	arg1	A					234:234	blood group A	222:234	blood group A	222:234	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	6	47	theme	related	1100:1106	arg1	transferases					1122:1133	evolutionarily related α1,3-Gal(NAc) transferases	1085:1133	evolutionarily related α1,3-Gal(NAc) transferases	1085:1133	We have expanded structural analysis to include evolutionarily related α1,3-Gal(NAc) transferases.
30696937	4	48	theme	amino	603:607	arg1	crucial					656:662	crucial	656:662	crucial	656:662	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	48	theme	amino	603:607	arg1	acids					609:613	the amino acids	599:613	the amino acids at codons 266 and 268 of human AT/BT	599:650	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	10	49	theme	functional	1736:1745	arg1	acids					1753:1757	functional amino acids	1736:1757	functional amino acids	1736:1757	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	7	50	theme	selected	1220:1227	arg1	tripeptides					1229:1239	selected tripeptides	1220:1239	selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly	1220:1277	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	50	theme	selected	1220:1227	arg1	MetGlyAla					1292:1300	BT-specific MetGlyAla	1280:1300	BT-specific MetGlyAla	1280:1300	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	50	theme	selected	1220:1227	arg1	iGb3S					1329:1333	iGb3S	1329:1333	iGb3S	1329:1333	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	50	theme	selected	1220:1227	arg1	GlyGlyAla					1315:1323	FS-specific GlyGlyAla	1303:1323	FS-specific GlyGlyAla	1303:1323	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	9	51	theme	transferase	1687:1697	arg1	activity					1699:1706	transferase activity	1687:1706	transferase activity	1687:1706	The results have shown that several tripeptides other than the originals also bestowed transferase activity.
30696937	7	52	theme	AT/BT	1410:1414	arg1	266-268					1393:1399	266-268	1393:1399	266-268	1393:1399	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	7	52	theme	AT/BT	1410:1414	arg1	AT/BT					1410:1414	human AT/BT	1404:1414	human AT/BT	1404:1414	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	4	53	from	268	633:635	arg1	crucial					656:662	crucial	656:662	crucial	656:662	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	53	from	268	633:635	arg1	acids					609:613	the amino acids	599:613	the amino acids at codons 266 and 268 of human AT/BT	599:650	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	11	54	from	pocket	2014:2019	arg1	sequences					1979:1987	different tripeptide sequences	1958:1987	different tripeptide sequences at the substrate-binding pocket	1958:2019	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	4	55	theme	human	640:644	arg1	AT/BT					646:650	human AT/BT	640:650	human AT/BT	640:650	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	0	56	theme	differential	123:134	arg1	activity					146:153	differential enzymatic activity	123:153	differential enzymatic activity	123:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	5	57	theme	sugar	1018:1022	arg1	specificity					1024:1034	sugar specificity	1018:1034	sugar specificity	1018:1034	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	9	58	dep	other	1648:1652	arg1	than					1654:1657	than	1654:1657	than	1654:1657	The results have shown that several tripeptides other than the originals also bestowed transferase activity.
30696937	1	59	theme	GGTA1	203:207	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	59	theme	GGTA1	203:207	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	59	theme	GGTA1	203:207	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	4	60	from	transfusion	807:817	arg1	basis					737:741	the molecular genetic basis	715:741	the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	715:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	8	61	theme	recipient	1466:1474	arg1	cells					1476:1480	appropriate recipient cells	1454:1480	appropriate recipient cells existing and newly created	1454:1507	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	4	62	theme	distinct	673:680	arg1	specificities					688:700	their distinct sugar specificities	667:700	their distinct sugar specificities	667:700	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	5	63	theme	possible	912:919	arg1	acids					927:931	20 possible amino acids	909:931	20 possible amino acids at those codons	909:947	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	8	64	theme	existing	1482:1489	arg1	cells					1476:1480	appropriate recipient cells	1454:1480	appropriate recipient cells existing and newly created	1454:1507	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	0	65	theme	group	70:74	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	5	66	theme	mutagenized	875:885	arg1	ATs/BTs					887:893	in vitro mutagenized ATs/BTs	866:893	in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons	866:947	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	0	67	theme	α1,3 Gal	91:98	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	1	68	theme	paralogous	167:176	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	68	theme	paralogous	167:176	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	68	theme	paralogous	167:176	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	0	69	theme	NAc	100:102	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	11	70	with	generation	2045:2054	arg1	specificities					2103:2115	diversified specificities	2091:2115	diversified specificities	2091:2115	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	4	71	from	basis	737:741	arg1	transfusion					807:817	transfusion	807:817	transfusion	807:817	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	4	71	from	basis	737:741	arg1	medicine					839:846	transplantation medicine	823:846	transplantation medicine	823:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	0	72	from	codons	46:51	arg1	substitutions					11:23	Amino acid substitutions	0:23	Amino acid substitutions at sugar-recognizing codons	0:51	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	11	73	theme	tripeptide	1968:1977	arg1	sequences					1979:1987	different tripeptide sequences	1958:1987	different tripeptide sequences at the substrate-binding pocket	1958:2019	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	0	74	theme	acid	6:9	arg1	substitutions					11:23	Amino acid substitutions	0:23	Amino acid substitutions at sugar-recognizing codons	0:51	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	6	75	theme	structural	1054:1063	arg1	analysis					1065:1072	structural analysis	1054:1072	structural analysis	1054:1072	We have expanded structural analysis to include evolutionarily related α1,3-Gal(NAc) transferases.
30696937	8	76	theme	surface	1537:1543	arg1	antigens					1561:1568	cell surface oligosaccharide antigens	1532:1568	cell surface oligosaccharide antigens	1532:1568	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	4	77	theme	clinical	784:791	arg1	importance					793:802	clinical importance	784:802	clinical importance	784:802	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	8	78	theme	antigens	1561:1568	arg1	appearance					1518:1527	the appearance	1514:1527	the appearance of cell surface oligosaccharide antigens	1514:1568	DNA transfection was performed using appropriate recipient cells existing and newly created, and the appearance of cell surface oligosaccharide antigens was immunologically examined.
30696937	10	79	theme	acceptor	1915:1922	arg1	substrates					1924:1933	donor nucleotide-sugar and acceptor substrates	1888:1933	substrates	1924:1933	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	4	80	theme	molecular	719:727	arg1	basis					737:741	the molecular genetic basis	715:741	the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	715:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	11	81	theme	substrate-binding	1996:2012	arg1	pocket					2014:2019	the substrate-binding pocket	1992:2019	the substrate-binding pocket	1992:2019	It was concluded that different tripeptide sequences at the substrate-binding pocket have contributed to the generation of α1,3-Gal(NAc) transferases with diversified specificities.
30696937	7	82	theme	expression	1147:1156	arg1	constructs					1158:1167	Eukaryotic expression constructs	1136:1167	Eukaryotic expression constructs	1136:1167	Eukaryotic expression constructs were prepared of AT, FS, iGb3S, and GT, possessing selected tripeptides of AT-specific AlaGlyGly or LeuGlyGly, BT-specific MetGlyAla, FS-specific GlyGlyAla, or iGb3S and GT-specific HisAlaAla, at the codons corresponding to 266-268 of human AT/BT.
30696937	5	83	theme	transferase	993:1003	arg1	activity					1005:1012	the transferase activity	989:1012	the transferase activity	989:1012	We also prepared in vitro mutagenized ATs/BTs having any of 20 possible amino acids at those codons, and showed that those codons determine the transferase activity and sugar specificity.
30696937	1	84	dep	A3GALT2	190:196	arg1	GBGT1					183:187	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	GBGT1	183:187	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	0	85	theme	ABO	60:62	arg1	transferases					105:116	ABO blood group system-related α1,3 Gal(NAc) transferases	60:116	ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity	60:153	Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity.
30696937	1	86	theme	Forssman	268:275	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	86	theme	Forssman	268:275	arg1	FS					298:299	FS	298:299	FS	298:299	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	86	theme	Forssman	268:275	arg1	synthase					288:295	Forssman glycolipid synthase	268:295	Forssman glycolipid synthase (FS)	268:300	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	4	87	theme	glycosylation	754:766	arg1	polymorphism					768:779	the ABO glycosylation polymorphism	746:779	the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine	746:846	Previously, we demonstrated that the amino acids at codons 266 and 268 of human AT/BT are crucial to their distinct sugar specificities, elucidating the molecular genetic basis of the ABO glycosylation polymorphism of clinical importance in transfusion and transplantation medicine.
30696937	1	88	theme	blood	222:226	arg1	A					234:234	blood group A	222:234	blood group A	222:234	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	6	89	theme	α1,3-Gal	1108:1115	arg1	transferases					1122:1133	evolutionarily related α1,3-Gal(NAc) transferases	1085:1133	evolutionarily related α1,3-Gal(NAc) transferases	1085:1133	We have expanded structural analysis to include evolutionarily related α1,3-Gal(NAc) transferases.
30696937	10	90	theme	amino	1747:1751	arg1	acids					1753:1757	functional amino acids	1736:1757	functional amino acids	1736:1757	However, the repertoire of functional amino acids varied among those transferases, suggesting that structures around those codons differentially affected the interactions between donor nucleotide-sugar and acceptor substrates.
30696937	1	91	theme	A	234:234	arg1	α1,3-galactosyltransferase					350:375	α1,3-galactosyltransferase	350:375	α1,3-galactosyltransferase (GT)	350:380	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	synthase					288:295	Forssman glycolipid synthase	268:295	Forssman glycolipid synthase (FS)	268:300	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	synthase					328:335	isoglobotriaosylceramide synthase	303:335	isoglobotriaosylceramide synthase (iGb3S)	303:343	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	AT					256:257	AT	256:257	AT	256:257	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	BT					263:264	BT	263:264	BT	263:264	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	A3GALT2					190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	A3GALT2	190:196	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	genes					209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes	156:213	genes	209:213	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
30696937	1	91	theme	A	234:234	arg1	transferases					242:253	blood group A and B transferases	222:253	blood group A and B transferases (AT and BT)	222:265	Functional paralogous ABO, GBGT1, A3GALT2, and GGTA1 genes encode blood group A and B transferases (AT and BT), Forssman glycolipid synthase (FS), isoglobotriaosylceramide synthase (iGb3S), and α1,3-galactosyltransferase (GT), respectively.
31369239	0	0	theme	Wearable	108:115	arg1	Sensors					117:123	High-Performance Wearable Sensors	91:123	High-Performance Wearable Sensors	91:123	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	3	1	with	applications	543:554	arg1	productivity					582:593	productivity	582:593	productivity	582:593	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	3	1	with	applications	543:554	arg1	reliability					566:576	high reliability	561:576	high reliability	561:576	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	1	2	theme	sensing	188:194	arg1	functionality					196:208	sensing functionality	188:208	sensing functionality	188:208	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	8	3	theme	wearable	1564:1571	arg1	sensors					1573:1579	versatile wearable sensors	1554:1579	versatile wearable sensors	1554:1579	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	0	4	theme	High-Performance	91:106	arg1	Sensors					117:123	High-Performance Wearable Sensors	91:123	High-Performance Wearable Sensors	91:123	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	4	5	theme	additional	760:769	arg1	processes					771:779	additional processes	760:779	additional processes	760:779	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	6	6	theme	TCNF/CNT	1090:1097	arg1	fibers					1099:1104	The TCNF/CNT fibers	1086:1104	The TCNF/CNT fibers	1086:1104	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	2	7	theme	sensing	331:337	arg1	fibers					339:344	previously reported wearable sensing fibers	302:344	previously reported wearable sensing fibers	302:344	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	1	8	theme	sensing	135:141	arg1	fibers					143:148	Weavable sensing fibers	126:148	Weavable sensing fibers with superior mechanical strength and sensing functionality	126:208	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	4	9	theme	distinct	827:834	arg1	nanocellulose					836:848	distinct nanocellulose	827:848	distinct nanocellulose	827:848	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	2	10	theme	wearable	322:329	arg1	fibers					339:344	previously reported wearable sensing fibers	302:344	previously reported wearable sensing fibers	302:344	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	4	11	theme	carbon	798:803	arg1	CNTs					816:819	CNTs	816:819	CNTs	816:819	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	4	11	theme	carbon	798:803	arg1	nanotubes					805:813	carbon nanotubes	798:813	carbon nanotubes (CNTs)	798:820	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	7	12	theme	weaving	1418:1424	arg1	process					1426:1432	a direct weaving process	1409:1432	a direct weaving process	1409:1432	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	2	13	theme	reported	313:320	arg1	fibers					339:344	previously reported wearable sensing fibers	302:344	previously reported wearable sensing fibers	302:344	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	4	14	theme	sensing	730:736	arg1	functionality					738:750	sensing functionality	730:750	sensing functionality	730:750	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	8	15	theme	meter-scale	1439:1449	arg1	synthesis					1460:1468	Our meter-scale scalable synthesis	1435:1468	Our meter-scale scalable synthesis of functional composite fibers	1435:1499	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	7	16	theme	harsh	1280:1284	arg1	distortions					1286:1296	complex and harsh distortions	1268:1296	complex and harsh distortions maintaining their intrinsic sensing properties	1268:1343	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	6	17	theme	superior	1116:1123	arg1	performance					1143:1153	a superior gas (NO2)-sensing performance	1114:1153	a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection)	1114:1221	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	4	18	theme	mechanical	704:713	arg1	properties					715:724	good mechanical properties	699:724	good mechanical properties	699:724	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	0	19	theme	Meter-Scale	11:21	arg1	Synthesis					23:31	Continuous Meter-Scale Synthesis	0:31	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.	0:124	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	7	20	theme	intrinsic	1316:1324	arg1	properties					1334:1343	their intrinsic sensing properties	1310:1343	their intrinsic sensing properties	1310:1343	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	1	21	with	fibers	143:148	arg1	strength					175:182	superior mechanical strength	155:182	superior mechanical strength	155:182	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	1	21	with	fibers	143:148	arg1	functionality					196:208	sensing functionality	188:208	sensing functionality	188:208	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	8	22	theme	fibers	1494:1499	arg1	synthesis					1460:1468	Our meter-scale scalable synthesis	1435:1468	Our meter-scale scalable synthesis of functional composite fibers	1435:1499	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	3	23	theme	sensing	456:462	arg1	fibers					464:469	The sensing fibers	452:469	The sensing fibers	452:469	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	0	24	theme	Continuous	0:9	arg1	Synthesis					23:31	Continuous Meter-Scale Synthesis	0:31	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.	0:124	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	7	25	theme	TCNF/CNT	1241:1248	arg1	fibers					1250:1255	the TCNF/CNT fibers	1237:1255	the TCNF/CNT fibers	1237:1255	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	2	26	theme	additional	347:356	arg1	reduction					376:384	reduction	376:384	reduction	376:384	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	2	26	theme	additional	347:356	arg1	coating					399:405	coating	399:405	coating	399:405	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	2	26	theme	additional	347:356	arg1	doping					387:392	doping	387:392	doping	387:392	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	2	26	theme	additional	347:356	arg1	processes					358:366	additional processes	347:366	additional processes such as reduction, doping, and coating	347:405	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	8	27	theme	functional	1473:1482	arg1	fibers					1494:1499	functional composite fibers	1473:1499	functional composite fibers	1473:1499	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	6	28	with	performance	1143:1153	arg1	sensitivity					1181:1191	sensitivity	1181:1191	sensitivity (parts-per-billion detection)	1181:1221	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	6	28	with	performance	1143:1153	arg1	detection					1212:1220	parts-per-billion detection	1194:1220	parts-per-billion detection	1194:1220	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	6	28	with	performance	1143:1153	arg1	selectivity					1165:1175	high selectivity	1160:1175	high selectivity	1160:1175	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	5	29	theme	single-walled	929:941	arg1	CNTs					943:946	single-walled CNTs	929:946	single-walled CNTs	929:946	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	5	30	theme	aligned	1010:1016	arg1	directions					1018:1027	aligned directions	1010:1027	aligned directions	1010:1027	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	1	31	theme	Weavable	126:133	arg1	fibers					143:148	Weavable sensing fibers	126:148	Weavable sensing fibers with superior mechanical strength and sensing functionality	126:208	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	6	32	theme	-sensing	1134:1141	arg1	performance					1143:1153	a superior gas (NO2)-sensing performance	1114:1153	a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection)	1114:1221	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	8	33	theme	composite	1484:1492	arg1	fibers					1494:1499	functional composite fibers	1473:1499	functional composite fibers	1473:1499	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	5	34	theme	liquid-crystal	1059:1072	arg1	properties					1074:1083	liquid-crystal properties	1059:1083	liquid-crystal properties	1059:1083	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	8	35	theme	sensors	1573:1579	arg1	platform					1542:1549	a mass production platform	1524:1549	a mass production platform of versatile wearable sensors	1524:1579	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	2	36	theme	fibers	339:344	arg1	fabrication					287:297	the fabrication	283:297	the fabrication of previously reported wearable sensing fibers	283:344	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	4	37	theme	sensing	679:685	arg1	fibers					687:692	mass-producible wearable sensing fibers	654:692	mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes	654:779	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	0	38	theme	Tunicate	45:52	arg1	Fibers					80:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	4	39	theme	good	699:702	arg1	properties					715:724	good mechanical properties	699:724	good mechanical properties	699:724	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	2	40	from	fabrication	287:297	arg1	essential					412:420	essential	412:420	essential	412:420	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	1	41	theme	wearable	245:252	arg1	sensors					262:268	wearable textile sensors	245:268	wearable textile sensors	245:268	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	0	42	theme	Weavable	36:43	arg1	Fibers					80:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	5	43	theme	composite	953:961	arg1	TCNF/CNT					971:978	TCNF/CNT	971:978	TCNF/CNT	971:978	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	5	43	theme	composite	953:961	arg1	fibers					963:968	composite fibers	953:968	composite fibers (TCNF/CNT)	953:979	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	2	44	from	essential	412:420	arg1	fabrication					287:297	the fabrication	283:297	the fabrication of previously reported wearable sensing fibers	283:344	However, in the fabrication of previously reported wearable sensing fibers, additional processes such as reduction, doping, and coating were essential to satisfy both requirements.
31369239	1	45	theme	textile	254:260	arg1	sensors					262:268	wearable textile sensors	245:268	wearable textile sensors	245:268	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	0	46	theme	Nanotube	71:78	arg1	Fibers					80:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	8	47	theme	mass	1526:1529	arg1	platform					1542:1549	a mass production platform	1524:1549	a mass production platform of versatile wearable sensors	1524:1579	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	6	48	theme	parts-per-billion	1194:1210	arg1	sensitivity					1181:1191	sensitivity	1181:1191	sensitivity (parts-per-billion detection)	1181:1221	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	6	48	theme	parts-per-billion	1194:1210	arg1	detection					1212:1220	parts-per-billion detection	1194:1220	parts-per-billion detection	1194:1220	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	7	49	theme	conventional	1382:1393	arg1	fabrics					1395:1401	conventional fabrics	1382:1401	conventional fabrics using a direct weaving process	1382:1432	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	0	50	theme	Cellulose/Carbon	54:69	arg1	Fibers					80:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Weavable Tunicate Cellulose/Carbon Nanotube Fibers	36:85	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	3	51	theme	commercial	532:541	arg1	applications					543:554	commercial applications	532:554	commercial applications	532:554	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	1	52	theme	superior	155:162	arg1	strength					175:182	superior mechanical strength	155:182	superior mechanical strength	155:182	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	5	53	theme	wet	1032:1034	arg1	spinning					1036:1043	wet spinning	1032:1043	wet spinning	1032:1043	Nanocellulose extracted from tunicate (TCNF) is homogeneously composited with single-walled CNTs, and composite fibers (TCNF/CNT) are continuously produced in aligned directions by wet spinning, facilitating liquid-crystal properties.
31369239	1	54	theme	sensors	262:268	arg1	realization					230:240	the realization	226:240	the realization of wearable textile sensors	226:268	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	4	55	with	fibers	687:692	arg1	properties					715:724	good mechanical properties	699:724	good mechanical properties	699:724	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	4	55	with	fibers	687:692	arg1	functionality					738:750	sensing functionality	730:750	sensing functionality	730:750	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	8	56	theme	production	1531:1540	arg1	platform					1542:1549	a mass production platform	1524:1549	a mass production platform of versatile wearable sensors	1524:1579	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	1	57	theme	mechanical	164:173	arg1	strength					175:182	superior mechanical strength	155:182	superior mechanical strength	155:182	Weavable sensing fibers with superior mechanical strength and sensing functionality are crucial for the realization of wearable textile sensors.
31369239	8	58	theme	scalable	1451:1458	arg1	synthesis					1460:1468	Our meter-scale scalable synthesis	1435:1468	Our meter-scale scalable synthesis of functional composite fibers	1435:1499	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	3	59	theme	scalable	511:518	arg1	process					520:526	a scalable process	509:526	a scalable process for commercial applications with high reliability and productivity, which was challenging	509:616	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	7	60	theme	sensing	1326:1332	arg1	properties					1334:1343	their intrinsic sensing properties	1310:1343	their intrinsic sensing properties	1310:1343	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	8	61	theme	versatile	1554:1562	arg1	sensors					1573:1579	versatile wearable sensors	1554:1579	versatile wearable sensors	1554:1579	Our meter-scale scalable synthesis of functional composite fibers is expected to provide a mass production platform of versatile wearable sensors.
31369239	7	62	theme	direct	1411:1416	arg1	process					1426:1432	a direct weaving process	1409:1432	a direct weaving process	1409:1432	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	3	63	theme	high	561:564	arg1	reliability					566:576	high reliability	561:576	high reliability	561:576	The sensing fibers should be continuously synthesized in a scalable process for commercial applications with high reliability and productivity, which was challenging.
31369239	6	64	theme	high	1160:1163	arg1	selectivity					1165:1175	high selectivity	1160:1175	high selectivity	1160:1175	The TCNF/CNT fibers exhibit a superior gas (NO2)-sensing performance with high selectivity and sensitivity (parts-per-billion detection).
31369239	4	65	theme	wearable	670:677	arg1	fibers					687:692	mass-producible wearable sensing fibers	654:692	mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes	654:779	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
31369239	0	66	theme	Fibers	80:85	arg1	Synthesis					23:31	Continuous Meter-Scale Synthesis	0:31	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.	0:124	Continuous Meter-Scale Synthesis of Weavable Tunicate Cellulose/Carbon Nanotube Fibers for High-Performance Wearable Sensors.
31369239	7	67	theme	complex	1268:1274	arg1	distortions					1286:1296	complex and harsh distortions	1268:1296	complex and harsh distortions maintaining their intrinsic sensing properties	1268:1343	In addition, the TCNF/CNT fibers can endure complex and harsh distortions maintaining their intrinsic sensing properties and can be perfectly integrated with conventional fabrics using a direct weaving process.
31369239	4	68	theme	mass-producible	654:668	arg1	fibers					687:692	mass-producible wearable sensing fibers	654:692	mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes	654:779	In this study, we first synthesize mass-producible wearable sensing fibers with good mechanical properties and sensing functionality without additional processes by incorporating carbon nanotubes (CNTs) into distinct nanocellulose.
30428408	5	0	theme	heparins	791:798	arg1	blending					779:786	blending	779:786	blending of heparins	779:798	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	1	1	attach	isolated	107:114	arg2	drug					102:105	a polysaccharide anticoagulant drug	71:105	a polysaccharide anticoagulant drug isolated from animal tissues	71:134	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	1	1	attach	isolated	107:114	arg1	tissues					128:134	animal tissues	121:134	animal tissues	121:134	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	1	1	attach	isolated	107:114	arg2	Heparin					60:66	Heparin	60:66	Heparin	60:66	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	4	2	theme	antithrombin	597:608	arg1	site					622:625	heparin's antithrombin III binding site	587:625	heparin's antithrombin III binding site	587:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	5	3	theme	various	805:811	arg1	sources					813:819	various sources	805:819	various sources	805:819	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	3	4	dep	porcine	350:356	arg1	ovine					370:374	ovine	370:374	ovine	370:374	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	3	4	dep	porcine	350:356	arg1	bovine					359:364	bovine	359:364	bovine	359:364	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	3	5	theme	analytical	325:334	arg1	techniques					336:345	modern analytical techniques	318:345	modern analytical techniques	318:345	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	4	6	theme	compositional	507:519	arg1	spectrometry					480:491	nuclear magnetic resonance spectrometry	453:491	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	6	theme	compositional	507:519	arg1	analysis					521:528	disaccharide compositional analysis	494:528	disaccharide compositional analysis	494:528	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	2	7	dep	safety	169:174	arg1	the					165:167	the	165:167	the	165:167	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	3	8	theme	techniques	336:345	arg1	suite					309:313	a suite	307:313	a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins	307:402	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	4	9	theme	magnetic	461:468	arg1	resonance					470:478	nuclear magnetic resonance	453:478	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	5	10	theme	structural	690:699	arg1	differences					701:711	these structural differences	684:711	these structural differences	684:711	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	5	11	theme	Chemometric	628:638	arg1	analysis					640:647	Chemometric analysis	628:647	Chemometric analysis	628:647	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	4	12	theme	nuclear	453:459	arg1	resonance					470:478	nuclear magnetic resonance	453:478	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	3	13	theme	modern	318:323	arg1	techniques					336:345	modern analytical techniques	318:345	modern analytical techniques	318:345	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	0	14	theme	bovine	33:38	arg1	heparins					50:57	bovine and ovine heparins	33:57	bovine and ovine heparins	33:57	Chemometric analysis of porcine, bovine and ovine heparins.
30428408	4	15	theme	resonance	470:478	arg1	analysis					541:548	bottom-up analysis	531:548	bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site	531:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	15	theme	resonance	470:478	arg1	spectrometry					480:491	nuclear magnetic resonance spectrometry	453:491	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	15	theme	resonance	470:478	arg1	analysis					521:528	disaccharide compositional analysis	494:528	disaccharide compositional analysis	494:528	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	0	16	theme	Chemometric	0:10	arg1	analysis					12:19	Chemometric analysis	0:19	Chemometric analysis of porcine	0:30	Chemometric analysis of porcine, bovine and ovine heparins.
30428408	1	17	theme	polysaccharide	73:86	arg1	Heparin					60:66	Heparin	60:66	Heparin	60:66	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	1	17	theme	polysaccharide	73:86	arg1	drug					102:105	a polysaccharide anticoagulant drug	71:105	a polysaccharide anticoagulant drug isolated from animal tissues	71:134	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	0	18	theme	porcine	24:30	arg1	analysis					12:19	Chemometric analysis	0:19	Chemometric analysis of porcine	0:30	Chemometric analysis of porcine, bovine and ovine heparins.
30428408	4	19	theme	tetrasaccharides	553:568	arg1	analysis					541:548	bottom-up analysis	531:548	bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site	531:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	19	theme	tetrasaccharides	553:568	arg1	spectrometry					480:491	nuclear magnetic resonance spectrometry	453:491	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	5	20	theme	animal/tissue	728:740	arg1	source					742:747	the animal/tissue source	724:747	the animal/tissue source of heparin	724:758	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	1	21	theme	anticoagulant	88:100	arg1	Heparin					60:66	Heparin	60:66	Heparin	60:66	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	1	21	theme	anticoagulant	88:100	arg1	drug					102:105	a polysaccharide anticoagulant drug	71:105	a polysaccharide anticoagulant drug isolated from animal tissues	71:134	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	3	22	theme	porcine	350:356	arg1	heparins					395:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	4	23	theme	structural	430:439	arg1	analysis					441:448	structural analysis	430:448	structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site	430:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	0	24	dep	heparins	50:57	arg1	analysis					12:19	Chemometric analysis	0:19	Chemometric analysis of porcine	0:30	Chemometric analysis of porcine, bovine and ovine heparins.
30428408	3	25	theme	intestinal	376:385	arg1	heparins					395:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	3	26	theme	mucosal	387:393	arg1	heparins					395:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	porcine, bovine and ovine intestinal mucosal heparins	350:402	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	4	27	theme	bottom-up	531:539	arg1	analysis					541:548	bottom-up analysis	531:548	bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site	531:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	27	theme	bottom-up	531:539	arg1	spectrometry					480:491	nuclear magnetic resonance spectrometry	453:491	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	5	28	theme	heparin	752:758	arg1	source					742:747	the animal/tissue source	724:747	the animal/tissue source of heparin	724:758	Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
30428408	2	29	theme	chain	211:215	arg1	security					180:187	security	180:187	security	180:187	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	2	29	theme	chain	211:215	arg1	safety					169:174	safety	169:174	safety	169:174	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	0	30	theme	ovine	44:48	arg1	heparins					50:57	bovine and ovine heparins	33:57	bovine and ovine heparins	33:57	Chemometric analysis of porcine, bovine and ovine heparins.
30428408	2	31	theme	supply	204:209	arg1	chain					211:215	the heparin supply chain	192:215	the heparin supply chain	192:215	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	3	32	theme	current	285:291	arg1	study					293:297	The current study	281:297	The current study	281:297	The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins.
30428408	2	33	theme	heparin	196:202	arg1	chain					211:215	the heparin supply chain	192:215	the heparin supply chain	192:215	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	2	34	theme	contamination	237:249	arg1	crisis					251:256	a contamination crisis	235:256	a contamination crisis	235:256	There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption.
30428408	1	35	theme	animal	121:126	arg1	tissues					128:134	animal tissues	121:134	animal tissues	121:134	Heparin is a polysaccharide anticoagulant drug isolated from animal tissues.
30428408	4	36	theme	binding	614:620	arg1	site					622:625	heparin's antithrombin III binding site	587:625	heparin's antithrombin III binding site	587:625	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	37	theme	disaccharide	494:505	arg1	spectrometry					480:491	nuclear magnetic resonance spectrometry	453:491	nuclear magnetic resonance spectrometry	453:491	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
30428408	4	37	theme	disaccharide	494:505	arg1	analysis					521:528	disaccharide compositional analysis	494:528	disaccharide compositional analysis	494:528	These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site.
31739048	1	0	theme	systems	183:189	arg1	development					154:164	the development	150:164	the development of drug delivery systems	150:189	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	1	theme	properties	258:267	arg1	range					239:243	a wide range	232:243	a wide range of colloidal properties	232:267	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	7	2	dep	formed	1190:1195	arg1	Rg/Rh = 1.0 ± 0.3					1198:1214	Rg/Rh = 1.0 ± 0.3	1198:1214	Rg/Rh = 1.0 ± 0.3	1198:1214	Disordered and structurally heterogeneous spherical complexes were formed (Rg/Rh = 1.0 ± 0.3).
31739048	6	3	theme	PEC	1050:1052	arg1	nanoparticles					1054:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	4	4	theme	tertiary	670:677	arg1	groups					685:690	the tertiary amino groups	666:690	the tertiary amino groups	666:690	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	9	5	theme	molecular	1422:1430	arg1	weight					1432:1437	the low molecular weight	1414:1437	the low molecular weight DEAE-CS	1414:1445	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	4	6	with	DEAE-CS	769:775	arg1	DS					786:787	the DS	782:787	the DS	782:787	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	6	7	theme	heterogeneous	1036:1048	arg1	nanoparticles					1054:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	5	8	theme	mixing	894:899	arg1	order					901:905	mixing order	894:905	mixing order	894:905	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	4	9	with	complexation	797:808	arg1	HA					832:833	HA	832:833	HA	832:833	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	9	with	complexation	797:808	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid (HA)	815:834	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	6	10	theme	primary	1109:1115	arg1	PECs					1117:1120	several primary PECs	1101:1120	several primary PECs	1101:1120	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	6	11	theme	several	1101:1107	arg1	PECs					1117:1120	several primary PECs	1101:1120	several primary PECs	1101:1120	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	5	12	theme	order	901:905	arg1	influence					841:849	The influence	837:849	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles	837:957	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	4	13	from	increases	737:745	arg1	ζ-potential					754:764	the ζ-potential	750:764	the ζ-potential of DEAE-CS with the DS	750:787	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	13	from	increases	737:745	arg1	complexation					797:808	the complexation	793:808	the complexation with hyaluronic acid (HA)	793:834	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	14	theme	amino	679:683	arg1	groups					685:690	the tertiary amino groups	666:690	the tertiary amino groups	666:690	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	15	with	ζ-potential	754:764	arg1	HA					832:833	HA	832:833	HA	832:833	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	15	with	ζ-potential	754:764	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid (HA)	815:834	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	5	16	theme	PEC	858:860	arg1	composition					862:872	the PEC composition	854:872	the PEC composition	854:872	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	17	from	influence	841:849	arg1	polydispersity					923:936	polydispersity	923:936	polydispersity	923:936	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	17	from	influence	841:849	arg1	size					914:917	size	914:917	size	914:917	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	3	18	theme	substitution	425:436	arg1	pattern					438:444	the substitution pattern	421:444	the substitution pattern of DEAE-CS	421:455	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	7	19	theme	heterogeneous	1151:1163	arg1	complexes					1175:1183	structurally heterogeneous spherical complexes	1138:1183	structurally heterogeneous spherical complexes	1138:1183	Disordered and structurally heterogeneous spherical complexes were formed (Rg/Rh = 1.0 ± 0.3).
31739048	2	20	theme	diethylaminoethyl	288:304	arg1	DEAE-CS					316:322	DEAE-CS	316:322	DEAE-CS	316:322	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	2	20	theme	diethylaminoethyl	288:304	arg1	chitosan					306:313	The water-soluble diethylaminoethyl chitosan	270:313	The water-soluble diethylaminoethyl chitosan (DEAE-CS)	270:323	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	6	21	theme	disordered	1025:1034	arg1	nanoparticles					1054:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	3	22	theme	pattern	438:444	arg1	Analysis					409:416	Analysis	409:416	Analysis of the substitution pattern of DEAE-CS by different NMR techniques	409:483	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	0	23	theme	chitosan-hyaluronic	18:36	arg1	acid					38:41	Diethylaminoethyl chitosan-hyaluronic acid	0:41	Diethylaminoethyl chitosan-hyaluronic acid	0:41	Diethylaminoethyl chitosan-hyaluronic acid polyelectrolyte complexes.
31739048	9	24	theme	weight	1325:1330	arg1	HA					1343:1344	HA	1343:1344	HA	1343:1344	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	9	24	theme	weight	1325:1330	arg1	component					1332:1340	The high molecular weight component	1306:1340	The high molecular weight component (HA)	1306:1345	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	3	25	theme	amino	559:563	arg1	substituent					583:593	the DEAE substituent	574:593	the DEAE substituent	574:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	25	theme	amino	559:563	arg1	group					565:569	the tertiary amino group	546:569	the tertiary amino group of the DEAE substituent	546:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	8	26	theme	obtained	1222:1229	arg1	metastable					1241:1250	metastable	1241:1250	metastable	1241:1250	The obtained PECs were metastable and their properties were influenced by mixing order.
31739048	8	26	theme	obtained	1222:1229	arg1	PECs					1231:1234	The obtained PECs	1218:1234	The obtained PECs	1218:1234	The obtained PECs were metastable and their properties were influenced by mixing order.
31739048	0	27	theme	Diethylaminoethyl	0:16	arg1	acid					38:41	Diethylaminoethyl chitosan-hyaluronic acid	0:41	Diethylaminoethyl chitosan-hyaluronic acid	0:41	Diethylaminoethyl chitosan-hyaluronic acid polyelectrolyte complexes.
31739048	9	28	dep	surface	1397:1403	arg1	DEAE-CS					1439:1445	the low molecular weight DEAE-CS	1414:1445	the low molecular weight DEAE-CS	1414:1445	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	4	29	theme	hyaluronic	815:824	arg1	HA					832:833	HA	832:833	HA	832:833	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	29	theme	hyaluronic	815:824	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid (HA)	815:834	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	3	30	theme	group	565:569	arg1	quaternization					528:541	quaternization	528:541	quaternization of the tertiary amino group of the DEAE substituent	528:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	30	theme	group	565:569	arg1	O-substitution					501:514	O-substitution	501:514	O-substitution	501:514	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	30	theme	group	565:569	arg1	N-					494:495	N-	494:495	N-	494:495	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	9	31	theme	minor	1356:1360	arg1	component					1362:1370	a minor component	1354:1370	a minor component	1354:1370	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	9	32	theme	high	1310:1313	arg1	weight					1325:1330	high molecular weight	1310:1330	The high molecular weight component (HA)	1306:1345	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	5	33	theme	DS	875:876	arg1	influence					841:849	The influence	837:849	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles	837:957	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	1	34	theme	great	131:135	arg1	interest					137:144	great interest	131:144	great interest for the development of drug delivery systems	131:189	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	5	35	theme	PEC	941:943	arg1	nanoparticles					945:957	PEC nanoparticles	941:957	PEC nanoparticles	941:957	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	4	36	theme	amino	708:712	arg1	groups					714:719	the quaternary amino groups	693:719	the quaternary amino groups	693:719	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	9	37	theme	molecular	1315:1323	arg1	weight					1325:1330	high molecular weight	1310:1330	The high molecular weight component (HA)	1306:1345	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	5	38	theme	DEAE-CS	881:887	arg1	composition					862:872	the PEC composition	854:872	the PEC composition	854:872	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	38	theme	DEAE-CS	881:887	arg1	DS					875:876	DS	875:876	DS of DEAE-CS	875:887	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	38	theme	DEAE-CS	881:887	arg1	order					901:905	mixing order	894:905	mixing order	894:905	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	3	39	theme	groups	629:634	arg1	fraction					600:607	the fraction	596:607	the fraction of quaternary amino groups	596:634	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	40	theme	tertiary	550:557	arg1	substituent					583:593	the DEAE substituent	574:593	the DEAE substituent	574:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	40	theme	tertiary	550:557	arg1	group					565:569	the tertiary amino group	546:569	the tertiary amino group of the DEAE substituent	546:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	41	theme	different	460:468	arg1	techniques					474:483	different NMR techniques	460:483	different NMR techniques	460:483	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	9	42	theme	weight	1432:1437	arg1	DEAE-CS					1439:1445	the low molecular weight DEAE-CS	1414:1445	the low molecular weight DEAE-CS	1414:1445	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	3	43	theme	DEAE	578:581	arg1	substituent					583:593	the DEAE substituent	574:593	the DEAE substituent	574:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	5	44	dep	size	914:917	arg1	the					910:912	the	910:912	the	910:912	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	6	45	theme	Internal	1016:1023	arg1	nanoparticles					1054:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles	1016:1066	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	3	46	theme	quaternary	612:621	arg1	groups					629:634	quaternary amino groups	612:634	quaternary amino groups	612:634	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	2	47	dep	113	403:405	arg1	to					400:401	to	400:401	to	400:401	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	3	48	theme	DEAE-CS	449:455	arg1	pattern					438:444	the substitution pattern	421:444	the substitution pattern of DEAE-CS	421:455	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	5	49	theme	light	998:1002	arg1	scattering					1004:1013	dynamic and static light scattering	979:1013	dynamic and static light scattering	979:1013	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	7	50	theme	spherical	1165:1173	arg1	complexes					1175:1183	structurally heterogeneous spherical complexes	1138:1183	structurally heterogeneous spherical complexes	1138:1183	Disordered and structurally heterogeneous spherical complexes were formed (Rg/Rh = 1.0 ± 0.3).
31739048	3	51	theme	amino	623:627	arg1	groups					629:634	quaternary amino groups	612:634	quaternary amino groups	612:634	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	2	52	theme	substitution	365:376	arg1	degrees					354:360	various degrees	346:360	various degrees	346:360	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	5	53	theme	composition	862:872	arg1	influence					841:849	The influence	837:849	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles	837:957	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	4	54	theme	DEAE-CS	769:775	arg1	ζ-potential					754:764	the ζ-potential	750:764	the ζ-potential of DEAE-CS with the DS	750:787	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	4	54	theme	DEAE-CS	769:775	arg1	complexation					797:808	the complexation	793:808	the complexation with hyaluronic acid (HA)	793:834	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	5	55	theme	nanoparticles	945:957	arg1	polydispersity					923:936	polydispersity	923:936	polydispersity	923:936	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	55	theme	nanoparticles	945:957	arg1	size					914:917	size	914:917	size	914:917	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	5	56	theme	dynamic	979:985	arg1	scattering					1004:1013	dynamic and static light scattering	979:1013	dynamic and static light scattering	979:1013	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	2	57	theme	water-soluble	274:286	arg1	DEAE-CS					316:322	DEAE-CS	316:322	DEAE-CS	316:322	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	2	57	theme	water-soluble	274:286	arg1	chitosan					306:313	The water-soluble diethylaminoethyl chitosan	270:313	The water-soluble diethylaminoethyl chitosan (DEAE-CS)	270:323	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	3	58	theme	substituent	583:593	arg1	substituent					583:593	the DEAE substituent	574:593	the DEAE substituent	574:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	58	theme	substituent	583:593	arg1	group					565:569	the tertiary amino group	546:569	the tertiary amino group of the DEAE substituent	546:593	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	3	59	theme	NMR	470:472	arg1	techniques					474:483	different NMR techniques	460:483	different NMR techniques	460:483	Analysis of the substitution pattern of DEAE-CS by different NMR techniques revealed N- and O-substitution, as well as quaternization of the tertiary amino group of the DEAE substituent; the fraction of quaternary amino groups increased with the DS.
31739048	1	60	theme	wide	234:237	arg1	range					239:243	a wide range	232:243	a wide range of colloidal properties	232:267	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	6	61	theme	PECs	1117:1120	arg1	aggregates					1087:1096	the aggregates	1083:1096	the aggregates of several primary PECs	1083:1120	Internal disordered heterogeneous PEC nanoparticles were formed by the aggregates of several primary PECs.
31739048	9	62	dep	DEAE-CS	1439:1445	arg1	was					1410:1412	was	1410:1412	was	1410:1412	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	9	62	dep	DEAE-CS	1439:1445	arg1	than					1405:1408	than	1405:1408	than	1405:1408	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	2	63	theme	various	346:352	arg1	degrees					354:360	various degrees	346:360	various degrees	346:360	The water-soluble diethylaminoethyl chitosan (DEAE-CS) was synthesized with various degrees of substitution (DS), ranging from 26 to 113%.
31739048	4	64	theme	quaternary	697:706	arg1	groups					714:719	the quaternary amino groups	693:719	the quaternary amino groups	693:719	Unlike the tertiary amino groups, the quaternary amino groups did not support increases in the ζ-potential of DEAE-CS with the DS and the complexation with hyaluronic acid (HA).
31739048	9	65	theme	low	1418:1420	arg1	weight					1432:1437	the low molecular weight	1414:1437	the low molecular weight DEAE-CS	1414:1445	The high molecular weight component (HA), being a minor component, was more exposed on the surface than was the low molecular weight DEAE-CS.
31739048	5	66	theme	static	991:996	arg1	scattering					1004:1013	dynamic and static light scattering	979:1013	dynamic and static light scattering	979:1013	The influence of the PEC composition, DS of DEAE-CS, and mixing order on the size and polydispersity of PEC nanoparticles was investigated by dynamic and static light scattering.
31739048	1	67	theme	Polysaccharide-based	70:89	arg1	PECs					118:121	PECs	118:121	PECs	118:121	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	67	theme	Polysaccharide-based	70:89	arg1	complexes					107:115	Polysaccharide-based polyelectrolyte complexes	70:115	Polysaccharide-based polyelectrolyte complexes (PECs)	70:122	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	68	theme	drug	169:172	arg1	systems					183:189	drug delivery systems	169:189	drug delivery systems	169:189	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	69	theme	polyelectrolyte	91:105	arg1	PECs					118:121	PECs	118:121	PECs	118:121	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	69	theme	polyelectrolyte	91:105	arg1	complexes					107:115	Polysaccharide-based polyelectrolyte complexes	70:115	Polysaccharide-based polyelectrolyte complexes (PECs)	70:122	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	70	theme	delivery	174:181	arg1	systems					183:189	drug delivery systems	169:189	drug delivery systems	169:189	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
31739048	1	71	theme	colloidal	248:256	arg1	properties					258:267	colloidal properties	248:267	colloidal properties	248:267	Polysaccharide-based polyelectrolyte complexes (PECs) are of great interest for the development of drug delivery systems, as they are easily prepared and exhibit a wide range of colloidal properties.
29747430	1	0	theme	unstable	308:315	arg1	properties					339:348	unstable physical and chemical properties	308:348	unstable physical and chemical properties	308:348	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	5	1	dep	modeling	801:808	arg1	results					810:816	results	810:816	results	810:816	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	4	2	from	NMR	769:771	arg1	data					747:750	data	747:750	data from FTIR and ¹H NMR	747:771	Most of the components in SAEO were successfully encapsulated by HPCD, which can be supported by data from FTIR and ¹H NMR.
29747430	6	3	theme	electronic	1044:1053	arg1	nose					1055:1058	electronic nose	1044:1058	electronic nose	1044:1058	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	1	4	theme	physical	317:324	arg1	properties					339:348	unstable physical and chemical properties	308:348	unstable physical and chemical properties	308:348	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	0	5	theme	Anise	117:121	arg1	Oil					133:135	Star Anise Essential Oil	112:135	Star Anise Essential Oil	112:135	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	0	6	from	Effect	0:5	arg1	Properties					98:107	Antibacterial Properties	84:107	Antibacterial Properties	84:107	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	0	6	from	Effect	0:5	arg1	Components					69:78	Components	69:78	Components	69:78	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	7	7	theme	SAEO	1324:1327	arg1	effect					1314:1319	the inhibition effect	1299:1319	the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability	1299:1422	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	0	8	theme	Star	112:115	arg1	Oil					133:135	Star Anise Essential Oil	112:135	Star Anise Essential Oil	112:135	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	1	9	theme	chemical	330:337	arg1	properties					339:348	unstable physical and chemical properties	308:348	unstable physical and chemical properties	308:348	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	4	10	from	FTIR	757:760	arg1	data					747:750	data	747:750	data from FTIR and ¹H NMR	747:771	Most of the components in SAEO were successfully encapsulated by HPCD, which can be supported by data from FTIR and ¹H NMR.
29747430	6	11	dep	makes	1161:1165	arg1	have					1181:1184	have	1181:1184	have a sustained release effect	1181:1211	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	0	12	theme	Oil	133:135	arg1	Properties					98:107	Antibacterial Properties	84:107	Antibacterial Properties	84:107	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	0	12	theme	Oil	133:135	arg1	Components					69:78	Components	69:78	Components	69:78	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	3	13	theme	GC-MS	527:531	arg1	results					533:539	The GC-MS results	523:539	The GC-MS results	523:539	The GC-MS results indicated that the compositions reduced and content of the compositions of SAEO changed after encapsulation.
29747430	2	14	theme	encapsulation	375:387	arg1	effect					355:360	The effect	351:360	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity	351:503	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	0	15	theme	Essential	123:131	arg1	Oil					133:135	Star Anise Essential Oil	112:135	Star Anise Essential Oil	112:135	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	2	16	theme	volatility	457:466	arg1	compositions					443:454	its compositions	439:454	its compositions	439:454	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	2	16	theme	volatility	457:466	arg1	stability					468:476	volatility stability	457:476	volatility stability	457:476	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	4	17	from	components	662:671	arg1	SAEO					676:679	SAEO	676:679	SAEO	676:679	Most of the components in SAEO were successfully encapsulated by HPCD, which can be supported by data from FTIR and ¹H NMR.
29747430	2	18	theme	selective	365:373	arg1	encapsulation					375:387	selective encapsulation	365:387	selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD)	365:434	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	7	19	theme	antibacterial	1400:1412	arg1	stability					1414:1422	its antibacterial stability	1396:1422	its antibacterial stability	1396:1422	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	1	20	theme	plant	175:179	arg1	oil					159:161	Star anise essential oil	138:161	Star anise essential oil (SAEO)	138:168	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	20	theme	plant	175:179	arg1	oil					191:193	a plant essential oil	173:193	a plant essential oil with good antibacterial activity	173:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	5	21	theme	molecular	791:799	arg1	modeling					801:808	the molecular modeling	787:808	the molecular modeling results	787:816	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	6	22	theme	release	1198:1204	arg1	effect					1206:1211	a sustained release effect	1186:1211	a sustained release effect	1186:1211	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	1	23	theme	essential	181:189	arg1	oil					159:161	Star anise essential oil	138:161	Star anise essential oil (SAEO)	138:168	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	23	theme	essential	181:189	arg1	oil					191:193	a plant essential oil	173:193	a plant essential oil with good antibacterial activity	173:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	0	24	theme	Encapsulation	20:32	arg1	Effect					0:5	Effect	0:5	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.	0:136	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	7	25	theme	inhibition	1303:1312	arg1	effect					1314:1319	the inhibition effect	1299:1319	the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability	1299:1422	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	6	26	theme	SAEO	991:994	arg1	stability					978:986	The volatile stability	965:986	The volatile stability of SAEO before and after encapsulation	965:1025	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	7	27	theme	Rhizopus	1332:1339	arg1	stolonoifer					1341:1351	Rhizopus stolonoifer	1332:1351	Rhizopus stolonoifer	1332:1351	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	0	28	theme	Selective	10:18	arg1	Encapsulation					20:32	Selective Encapsulation	10:32	Selective Encapsulation of Hydroxypropyl-β-cyclodextrin	10:64	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	1	29	theme	high	273:276	arg1	volatility					278:287	its high volatility	269:287	its high volatility	269:287	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	4	30	theme	¹H	766:767	arg1	NMR					769:771	¹H NMR	766:771	¹H NMR	766:771	Most of the components in SAEO were successfully encapsulated by HPCD, which can be supported by data from FTIR and ¹H NMR.
29747430	5	31	theme	HPCD	927:930	arg1	cavity					917:922	the cavity	913:922	the cavity of HPCD on the isoallyl (or allyl) side	913:962	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	7	32	theme	selective	1258:1266	arg1	encapsulation					1268:1280	the selective encapsulation	1254:1280	the selective encapsulation of HPCD	1254:1288	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	0	33	theme	Hydroxypropyl-β-cyclodextrin	37:64	arg1	Encapsulation					20:32	Selective Encapsulation	10:32	Selective Encapsulation of Hydroxypropyl-β-cyclodextrin	10:64	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	7	34	theme	antibacterial	1234:1246	arg1	test					1248:1251	the antibacterial test	1230:1251	the antibacterial test	1230:1251	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	1	35	with	oil	191:193	arg1	activity					219:226	good antibacterial activity	200:226	good antibacterial activity	200:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	2	36	theme	SAEO	392:395	arg1	encapsulation					375:387	selective encapsulation	365:387	selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD)	365:434	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	6	37	theme	sustained	1188:1196	arg1	effect					1206:1211	a sustained release effect	1186:1211	a sustained release effect	1186:1211	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	1	38	theme	good	200:203	arg1	activity					219:226	good antibacterial activity	200:226	good antibacterial activity	200:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	7	39	theme	HPCD	1285:1288	arg1	encapsulation					1268:1280	the selective encapsulation	1254:1280	the selective encapsulation of HPCD	1254:1288	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	3	40	theme	compositions	600:611	arg1	compositions					560:571	the compositions	556:571	the compositions reduced	556:579	The GC-MS results indicated that the compositions reduced and content of the compositions of SAEO changed after encapsulation.
29747430	3	40	theme	compositions	600:611	arg1	content					585:591	content	585:591	content of the compositions of SAEO	585:619	The GC-MS results indicated that the compositions reduced and content of the compositions of SAEO changed after encapsulation.
29747430	1	41	theme	antibacterial	205:217	arg1	activity					219:226	good antibacterial activity	200:226	good antibacterial activity	200:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	42	theme	strong	290:295	arg1	smell					297:301	strong smell	290:301	strong smell	290:301	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	5	43	dep	molecules	835:843	arg1	trans-foeniculin					876:891	trans-foeniculin	876:891	trans-foeniculin	876:891	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	43	dep	molecules	835:843	arg1	estragole					862:870	estragole	862:870	estragole	862:870	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	43	dep	molecules	835:843	arg1	trans-anethole					846:859	trans-anethole	846:859	trans-anethole	846:859	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	43	dep	molecules	835:843	arg1	molecules					835:843	the three guest molecules	819:843	the three guest molecules (trans-anethole, estragole and trans-foeniculin)	819:892	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	2	44	from	effect	355:360	arg1	compositions					443:454	its compositions	439:454	its compositions	439:454	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	2	44	from	effect	355:360	arg1	stability					468:476	volatility stability	457:476	volatility stability	457:476	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	2	44	from	effect	355:360	arg1	activity					496:503	antibacterial activity	482:503	antibacterial activity	482:503	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	1	45	theme	Star	138:141	arg1	SAEO					164:167	SAEO	164:167	SAEO	164:167	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	45	theme	Star	138:141	arg1	oil					191:193	a plant essential oil	173:193	a plant essential oil with good antibacterial activity	173:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	45	theme	Star	138:141	arg1	oil					159:161	Star anise essential oil	138:161	Star anise essential oil (SAEO)	138:168	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	6	46	theme	SAEO	1152:1155	arg1	smell					1143:1147	the irritating smell	1128:1147	the irritating smell of SAEO	1128:1155	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	6	47	theme	irritating	1132:1141	arg1	smell					1143:1147	the irritating smell	1128:1147	the irritating smell of SAEO	1128:1155	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	1	48	theme	anise	143:147	arg1	SAEO					164:167	SAEO	164:167	SAEO	164:167	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	48	theme	anise	143:147	arg1	oil					191:193	a plant essential oil	173:193	a plant essential oil with good antibacterial activity	173:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	48	theme	anise	143:147	arg1	oil					159:161	Star anise essential oil	138:161	Star anise essential oil (SAEO)	138:168	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	5	49	theme	allyl	952:956	arg1	side					959:962	the isoallyl (or allyl) side	935:962	side	959:962	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	1	50	theme	essential	149:157	arg1	SAEO					164:167	SAEO	164:167	SAEO	164:167	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	50	theme	essential	149:157	arg1	oil					191:193	a plant essential oil	173:193	a plant essential oil with good antibacterial activity	173:226	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	1	50	theme	essential	149:157	arg1	oil					159:161	Star anise essential oil	138:161	Star anise essential oil (SAEO)	138:168	Star anise essential oil (SAEO) is a plant essential oil with good antibacterial activity, but its applications are limited due to its high volatility, strong smell, and unstable physical and chemical properties.
29747430	6	51	theme	volatile	969:976	arg1	stability					978:986	The volatile stability	965:986	The volatile stability of SAEO before and after encapsulation	965:1025	The volatile stability of SAEO before and after encapsulation was evaluated by electronic nose, and the results confirmed that encapsulation significantly reduced the irritating smell of SAEO and makes the clathrate have a sustained release effect.
29747430	0	52	theme	Antibacterial	84:96	arg1	Properties					98:107	Antibacterial Properties	84:107	Antibacterial Properties	84:107	Effect of Selective Encapsulation of Hydroxypropyl-β-cyclodextrin on Components and Antibacterial Properties of Star Anise Essential Oil.
29747430	5	53	theme	guest	829:833	arg1	trans-foeniculin					876:891	trans-foeniculin	876:891	trans-foeniculin	876:891	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	53	theme	guest	829:833	arg1	estragole					862:870	estragole	862:870	estragole	862:870	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	53	theme	guest	829:833	arg1	trans-anethole					846:859	trans-anethole	846:859	trans-anethole	846:859	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	53	theme	guest	829:833	arg1	molecules					835:843	the three guest molecules	819:843	the three guest molecules (trans-anethole, estragole and trans-foeniculin)	819:892	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	54	from	cavity	917:922	arg1	side					959:962	the isoallyl (or allyl) side	935:962	side	959:962	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	5	54	from	cavity	917:922	arg1	isoallyl					939:946	the isoallyl (or allyl) side	935:962	isoallyl	939:946	According to the molecular modeling results, the three guest molecules (trans-anethole, estragole and trans-foeniculin) were all docked in the cavity of HPCD on the isoallyl (or allyl) side.
29747430	2	55	theme	antibacterial	482:494	arg1	compositions					443:454	its compositions	439:454	its compositions	439:454	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	2	55	theme	antibacterial	482:494	arg1	activity					496:503	antibacterial activity	482:503	antibacterial activity	482:503	The effect of selective encapsulation of SAEO by hydroxypropyl-β-cyclodextrin (HPCD) on its compositions, volatility stability and antibacterial activity was investigated.
29747430	7	56	from	effect	1314:1319	arg1	stability					1414:1422	its antibacterial stability	1396:1422	its antibacterial stability	1396:1422	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	7	56	from	effect	1314:1319	arg1	cerevisiae					1368:1377	Saccharomyces cerevisiae	1354:1377	Saccharomyces cerevisiae	1354:1377	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	7	56	from	effect	1314:1319	arg1	coli					1387:1390	E. coli	1384:1390	E. coli	1384:1390	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	7	56	from	effect	1314:1319	arg1	stolonoifer					1341:1351	Rhizopus stolonoifer	1332:1351	Rhizopus stolonoifer	1332:1351	Furthermore, in the antibacterial test, the selective encapsulation of HPCD improved the inhibition effect of SAEO on Rhizopus stolonoifer, Saccharomyces cerevisiae, and E. coli and its antibacterial stability in 24 h.
29747430	3	57	theme	SAEO	616:619	arg1	compositions					600:611	the compositions	596:611	the compositions of SAEO	596:619	The GC-MS results indicated that the compositions reduced and content of the compositions of SAEO changed after encapsulation.
30659881	0	0	theme	promising	96:104	arg1	purpose					124:130	promising lacrimal plugging purpose	96:130	promising lacrimal plugging purpose	96:130	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	5	1	theme	composite	722:730	arg1	hydrogel					732:739	the composite hydrogel	718:739	the composite hydrogel	718:739	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	3	2	theme	suitable	486:493	arg1	ratio					504:508	a suitable swelling ratio	484:508	a suitable swelling ratio	484:508	The PVA/KGM/SA composite hydrogel showed a suitable swelling ratio and rate, as well as elasticity and flexibility.
30659881	8	3	theme	simple	1194:1199	arg1	method					1222:1227	a simple, effective, and safe method	1192:1227	a simple, effective, and safe method for the preparation of hydrogels	1192:1260	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	2	4	theme	glucomannan	339:349	arg1	mix					325:327	a mix	323:327	a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide	323:440	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	0	5	theme	plugging	115:122	arg1	purpose					124:130	promising lacrimal plugging purpose	96:130	promising lacrimal plugging purpose	96:130	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	8	6	theme	potential	1277:1285	arg1	applications					1287:1298	potential applications	1277:1298	potential applications	1277:1298	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	5	7	dep	elasticity	857:866	arg1	the					853:855	the	853:855	the	853:855	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	0	8	theme	lacrimal	106:113	arg1	purpose					124:130	promising lacrimal plugging purpose	96:130	promising lacrimal plugging purpose	96:130	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	3	9	theme	composite	458:466	arg1	hydrogel					468:475	The PVA/KGM/SA composite hydrogel	443:475	The PVA/KGM/SA composite hydrogel	443:475	The PVA/KGM/SA composite hydrogel showed a suitable swelling ratio and rate, as well as elasticity and flexibility.
30659881	5	10	theme	Rheological	686:696	arg1	studies					698:704	Rheological studies	686:704	Rheological studies	686:704	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	5	11	theme	chemical	799:806	arg1	interactions					821:832	chemical and physical interactions	799:832	chemical and physical interactions	799:832	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	2	12	theme	alcohol	394:400	arg1	mix					325:327	a mix	323:327	a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide	323:440	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	4	13	theme	breaking	615:622	arg1	strength					624:631	a breaking strength	613:631	a breaking strength of 87.25 kPa	613:644	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	7	14	theme	tear	1058:1061	arg1	height					1072:1077	the normal tear meniscus height	1047:1077	the normal tear meniscus height	1047:1077	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	7	15	theme	tear	1101:1104	arg1	area					1115:1118	the low tear meniscus area	1093:1118	the low tear meniscus area	1093:1118	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	7	16	theme	meniscus	1063:1070	arg1	height					1072:1077	the normal tear meniscus height	1047:1077	the normal tear meniscus height	1047:1077	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	2	17	theme	composite	283:291	arg1	hydrogels					293:301	composite hydrogels	283:301	composite hydrogels	283:301	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	8	18	contain	have	1272:1275	arg1	gels					1170:1173	the PVA/KGM/SA gels	1155:1173	the PVA/KGM/SA gels	1155:1173	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	8	18	contain	have	1272:1275	arg2	applications					1287:1298	potential applications	1277:1298	potential applications	1277:1298	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	1	19	theme	important	195:203	arg1	flexibility					164:174	flexibility	164:174	flexibility	164:174	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	19	theme	important	195:203	arg1	toughness					181:189	toughness	181:189	toughness	181:189	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	19	theme	important	195:203	arg1	parameters					205:214	important parameters	195:214	important parameters in hydrogel preparation for application in the human body	195:272	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	19	theme	important	195:203	arg1	strength					154:161	mechanical strength	143:161	mechanical strength	143:161	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	7	20	theme	normal	1051:1056	arg1	height					1072:1077	the normal tear meniscus height	1047:1077	the normal tear meniscus height	1047:1077	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	4	21	theme	1.660 MPa	675:683	arg1	strength					624:631	a breaking strength	613:631	a breaking strength of 87.25 kPa	613:644	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	4	21	theme	1.660 MPa	675:683	arg1	modulus					664:670	a compression modulus	650:670	a compression modulus of 1.660 MPa	650:683	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	4	22	from	%	605:605	arg1	addition					562:569	addition	562:569	addition	562:569	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	5	23	theme	cross-linked	768:779	arg1	network					781:787	a multiply cross-linked network	757:787	a multiply cross-linked network involving chemical and physical interactions	757:832	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	8	24	theme	PVA/KGM/SA	1159:1168	arg1	gels					1170:1173	the PVA/KGM/SA gels	1155:1173	the PVA/KGM/SA gels	1155:1173	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	8	25	theme	dry	1320:1322	arg1	syndrome					1328:1335	dry eye syndrome	1320:1335	dry eye syndrome	1320:1335	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	0	26	theme	Konjac	15:20	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Konjac	0:20	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	2	27	theme	alginate	365:372	arg1	mix					325:327	a mix	323:327	a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide	323:440	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	4	28	theme	87.25 kPa	636:644	arg1	strength					624:631	a breaking strength	613:631	a breaking strength of 87.25 kPa	613:644	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	4	28	theme	87.25 kPa	636:644	arg1	modulus					664:670	a compression modulus	650:670	a compression modulus of 1.660 MPa	650:683	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	1	29	theme	hydrogel	219:226	arg1	preparation					228:238	hydrogel preparation	219:238	hydrogel preparation	219:238	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	7	30	theme	meniscus	1106:1113	arg1	area					1115:1118	the low tear meniscus area	1093:1118	the low tear meniscus area	1093:1118	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	6	31	theme	gel	925:927	arg1	network					929:935	the composite gel network	911:935	the composite gel network	911:935	Interestingly, the composite gel network was reformed when the temperature decreased.
30659881	6	31	theme	gel	925:927	arg1	reformed					941:948	reformed	941:948	reformed	941:948	Interestingly, the composite gel network was reformed when the temperature decreased.
30659881	8	32	theme	syndrome	1328:1335	arg1	treatment					1307:1315	the treatment	1303:1315	the treatment of dry eye syndrome	1303:1335	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	4	33	from	break	590:594	arg1	%					605:605	660.3%	600:605	660.3%	600:605	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	4	33	from	break	590:594	arg1	elongation					576:585	the elongation	572:585	the elongation at break	572:594	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	2	34	theme	polyvinyl	384:392	arg1	PVA					403:405	PVA	403:405	PVA	403:405	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	2	34	theme	polyvinyl	384:392	arg1	alcohol					394:400	polyvinyl alcohol	384:400	polyvinyl alcohol (PVA)	384:406	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	0	35	theme	composite	40:48	arg1	hydrogel					50:57	composite hydrogel	40:57	composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose	40:130	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	6	36	theme	composite	915:923	arg1	network					929:935	the composite gel network	911:935	the composite gel network	911:935	Interestingly, the composite gel network was reformed when the temperature decreased.
30659881	6	36	theme	composite	915:923	arg1	reformed					941:948	reformed	941:948	reformed	941:948	Interestingly, the composite gel network was reformed when the temperature decreased.
30659881	8	37	theme	hydrogels	1252:1260	arg1	preparation					1237:1247	the preparation	1233:1247	the preparation of hydrogels	1233:1260	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	8	38	theme	eye	1324:1326	arg1	syndrome					1328:1335	dry eye syndrome	1320:1335	dry eye syndrome	1320:1335	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	7	39	theme	rabbit	985:990	arg1	models					992:997	rabbit models	985:997	rabbit models of dry eye	985:1008	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	5	40	theme	physical	812:819	arg1	interactions					821:832	chemical and physical interactions	799:832	chemical and physical interactions	799:832	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	7	41	theme	low	1097:1099	arg1	area					1115:1118	the low tear meniscus area	1093:1118	the low tear meniscus area	1093:1118	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	7	42	theme	eye	1006:1008	arg1	models					992:997	rabbit models	985:997	rabbit models of dry eye	985:1008	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	4	43	theme	compression	652:662	arg1	modulus					664:670	a compression modulus	650:670	a compression modulus of 1.660 MPa	650:683	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	2	44	theme	calcium	424:430	arg1	hydroxide					432:440	calcium hydroxide	424:440	calcium hydroxide	424:440	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	4	45	with	%	605:605	arg1	strength					624:631	a breaking strength	613:631	a breaking strength of 87.25 kPa	613:644	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	4	45	with	%	605:605	arg1	modulus					664:670	a compression modulus	650:670	a compression modulus of 1.660 MPa	650:683	In addition, the elongation at break was 660.3%, with a breaking strength of 87.25 kPa and a compression modulus of 1.660 MPa.
30659881	5	46	theme	gel	891:893	arg1	flexibility					872:882	flexibility	872:882	flexibility	872:882	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	5	46	theme	gel	891:893	arg1	elasticity					857:866	elasticity	857:866	elasticity	857:866	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	1	47	from	application	244:254	arg1	body					269:272	the human body	259:272	the human body	259:272	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	5	48	link	cross-linked	768:779	arg1	network					781:787	a multiply cross-linked network	757:787	a multiply cross-linked network involving chemical and physical interactions	757:832	Rheological studies showed that the composite hydrogel was composed of a multiply cross-linked network involving chemical and physical interactions, thereby affecting the elasticity and flexibility of the gel.
30659881	8	49	theme	effective	1202:1210	arg1	method					1222:1227	a simple, effective, and safe method	1192:1227	a simple, effective, and safe method for the preparation of hydrogels	1192:1260	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	1	50	theme	mechanical	143:152	arg1	flexibility					164:174	flexibility	164:174	flexibility	164:174	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	50	theme	mechanical	143:152	arg1	toughness					181:189	toughness	181:189	toughness	181:189	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	50	theme	mechanical	143:152	arg1	strength					154:161	mechanical strength	143:161	mechanical strength	143:161	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	1	50	theme	mechanical	143:152	arg1	parameters					205:214	important parameters	195:214	important parameters in hydrogel preparation for application in the human body	195:272	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	7	51	theme	dry	1002:1004	arg1	eye					1006:1008	dry eye	1002:1008	dry eye	1002:1008	In rabbit models of dry eye, the hydrogel effectively maintained the normal tear meniscus height and increased the low tear meniscus area.
30659881	2	52	theme	Konjac	332:337	arg1	KGM					352:354	KGM	352:354	KGM	352:354	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	2	52	theme	Konjac	332:337	arg1	glucomannan					339:349	Konjac glucomannan	332:349	Konjac glucomannan (KGM)	332:355	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	3	53	theme	PVA/KGM/SA	447:456	arg1	hydrogel					468:475	The PVA/KGM/SA composite hydrogel	443:475	The PVA/KGM/SA composite hydrogel	443:475	The PVA/KGM/SA composite hydrogel showed a suitable swelling ratio and rate, as well as elasticity and flexibility.
30659881	8	54	theme	safe	1217:1220	arg1	method					1222:1227	a simple, effective, and safe method	1192:1227	a simple, effective, and safe method for the preparation of hydrogels	1192:1260	The results therefore showed that the PVA/KGM/SA gels not only provide a simple, effective, and safe method for the preparation of hydrogels, but also have potential applications in the treatment of dry eye syndrome.
30659881	1	55	from	parameters	205:214	arg1	preparation					228:238	hydrogel preparation	219:238	hydrogel preparation	219:238	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30659881	3	56	theme	swelling	495:502	arg1	ratio					504:508	a suitable swelling ratio	484:508	a suitable swelling ratio	484:508	The PVA/KGM/SA composite hydrogel showed a suitable swelling ratio and rate, as well as elasticity and flexibility.
30659881	0	57	theme	calcium	74:80	arg1	hydroxide					82:90	calcium hydroxide	74:90	calcium hydroxide for promising lacrimal plugging purpose	74:130	Fabrication of Konjac glucomannan-based composite hydrogel crosslinked by calcium hydroxide for promising lacrimal plugging purpose.
30659881	2	58	theme	sodium	358:363	arg1	SA					375:376	SA	375:376	SA	375:376	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	2	58	theme	sodium	358:363	arg1	alginate					365:372	sodium alginate	358:372	sodium alginate (SA)	358:377	Herein, composite hydrogels were prepared using a mix of Konjac glucomannan (KGM), sodium alginate (SA), and polyvinyl alcohol (PVA) cross-linked by calcium hydroxide.
30659881	1	59	theme	human	263:267	arg1	body					269:272	the human body	259:272	the human body	259:272	Swelling, mechanical strength, flexibility, and toughness are important parameters in hydrogel preparation for application in the human body.
30120580	6	0	theme	filler	919:924	arg1	concentration					926:938	filler concentration	919:938	filler concentration	919:938	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	9	1	dep	CHS	1231:1233	arg1	material					1249:1256	the composite material	1235:1256	up to 0.6% CHS the composite material	1220:1256	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	9	1	dep	CHS	1231:1233	arg1	%					1229:1229	%	1229:1229	%	1229:1229	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	5	2	theme	SEM	757:759	arg1	micrograph					761:770	the SEM micrograph	753:770	the SEM micrograph	753:770	The fiber formation and the reduction in fiber diameter were confirmed by the SEM micrograph.
30120580	4	3	theme	chitosan	530:537	arg1	addition					518:525	The addition	514:525	The addition	514:525	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	6	4	theme	contact	808:814	arg1	measurements					822:833	contact angle measurements	808:833	contact angle measurements	808:833	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	5	5	from	reduction	707:715	arg1	diameter					726:733	fiber diameter	720:733	fiber diameter	720:733	The fiber formation and the reduction in fiber diameter were confirmed by the SEM micrograph.
30120580	9	6	theme	composite	1239:1247	arg1	material					1249:1256	the composite material	1235:1256	up to 0.6% CHS the composite material	1220:1256	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	7	7	dep	Fourier	1004:1010	arg1	transform					1012:1020	transform	1012:1020	transform infrared spectroscopy and thermal analysis	1012:1063	The weak interaction between PLA and chitosan was confirmed by Fourier transform infrared spectroscopy and thermal analysis.
30120580	10	8	theme	environmental	1413:1425	arg1	applications					1442:1453	environmental and biomedical applications	1413:1453	environmental and biomedical applications	1413:1453	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	4	9	from	CHS	674:676	arg1	optimum					656:662	an optimum	653:662	an optimum at 0.4wt% CHS	653:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	5	10	from	formation	689:697	arg1	diameter					726:733	fiber diameter	720:733	fiber diameter	720:733	The fiber formation and the reduction in fiber diameter were confirmed by the SEM micrograph.
30120580	0	11	theme	advanced	101:108	arg1	application					110:120	advanced application	101:120	advanced application	101:120	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	7	12	dep	transform	1012:1020	arg1	infrared					1022:1029	infrared	1022:1029	transform infrared spectroscopy and thermal analysis	1012:1063	The weak interaction between PLA and chitosan was confirmed by Fourier transform infrared spectroscopy and thermal analysis.
30120580	3	13	theme	solvent	498:504	arg1	system					506:511	a mixed solvent system	490:511	a mixed solvent system	490:511	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	6	14	theme	membrane	893:900	arg1	hydrophobicity					861:874	hydrophobicity	861:874	hydrophobicity of the composite membrane	861:900	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	6	15	theme	gas	785:787	arg1	chromatography					789:802	The inverse gas chromatography	773:802	The inverse gas chromatography	773:802	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	4	16	theme	%	672:672	arg1	CHS					674:676	0.4wt% CHS	667:676	0.4wt% CHS	667:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	6	17	theme	composite	883:891	arg1	membrane					893:900	the composite membrane	879:900	the composite membrane	879:900	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	6	18	theme	inverse	777:783	arg1	chromatography					789:802	The inverse gas chromatography	773:802	The inverse gas chromatography	773:802	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	3	19	theme	chemical	390:397	arg1	characterization					399:414	the physical and chemical characterization	373:414	the physical and chemical characterization	373:414	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	8	20	theme	composite	1087:1095	arg1	stability					1070:1078	The stability	1066:1078	The stability of the composite	1066:1095	The stability of the composite was established by zeta potential measurements.
30120580	1	21	theme	biodegradable	205:217	arg1	materials					219:227	biodegradable materials	205:227	biodegradable materials	205:227	Fabricating novel materials for biomedical applications mostly require the use of biodegradable materials.
30120580	0	22	theme	Electrospun	0:10	arg1	composite					37:45	Electrospun polylactic acid-chitosan composite	0:45	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.	0:121	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	10	23	theme	current	1280:1286	arg1	findings					1288:1295	The current findings	1276:1295	The current findings	1276:1295	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	10	23	theme	current	1280:1286	arg1	important					1306:1314	important	1306:1314	important	1306:1314	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	9	24	theme	Cytotoxicity	1145:1156	arg1	studies					1158:1164	Cytotoxicity studies	1145:1164	Cytotoxicity studies of the membranes	1145:1181	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	1	25	theme	materials	219:227	arg1	use					198:200	the use	194:200	the use of biodegradable materials	194:227	Fabricating novel materials for biomedical applications mostly require the use of biodegradable materials.
30120580	3	26	theme	physical	377:384	arg1	characterization					399:414	the physical and chemical characterization	373:414	the physical and chemical characterization	373:414	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	10	27	theme	biomedical	1431:1440	arg1	applications					1442:1453	environmental and biomedical applications	1413:1453	environmental and biomedical applications	1413:1453	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	4	28	theme	fibers	641:646	arg1	distribution					625:636	the distribution	621:636	the distribution of fibers with an optimum at 0.4wt% CHS	621:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	0	29	theme	acid-chitosan	23:35	arg1	composite					37:45	Electrospun polylactic acid-chitosan composite	0:45	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.	0:121	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	1	30	theme	Fabricating	123:133	arg1	materials					141:149	Fabricating novel materials	123:149	Fabricating novel materials for biomedical applications	123:177	Fabricating novel materials for biomedical applications mostly require the use of biodegradable materials.
30120580	9	31	dep	0.6	1226:1228	arg1	to					1223:1224	to	1223:1224	to	1223:1224	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	3	32	theme	PLA-CHS	423:429	arg1	composite					431:439	the PLA-CHS composite	419:439	the PLA-CHS composite	419:439	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	4	33	theme	fiber	569:573	arg1	diameter					575:582	fiber diameter	569:582	fiber diameter	569:582	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	8	34	theme	zeta	1116:1119	arg1	measurements					1131:1142	zeta potential measurements	1116:1142	zeta potential measurements	1116:1142	The stability of the composite was established by zeta potential measurements.
30120580	6	35	theme	hydrophobicity	861:874	arg1	increase					849:856	the increase	845:856	the increase of hydrophobicity of the composite membrane	845:900	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	2	36	theme	polylactic	272:281	arg1	PLA					289:291	PLA	289:291	PLA	289:291	In this work biodegradable materials like polylactic acid (PLA) and chitosan (CHS) were used for designing electrospun mats.
30120580	2	36	theme	polylactic	272:281	arg1	acid					283:286	polylactic acid	272:286	polylactic acid (PLA)	272:292	In this work biodegradable materials like polylactic acid (PLA) and chitosan (CHS) were used for designing electrospun mats.
30120580	4	37	theme	0.4wt	667:671	arg1	CHS					674:676	0.4wt% CHS	667:676	0.4wt% CHS	667:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	3	38	theme	electrospinning	458:472	arg1	technique					474:482	the electrospinning technique	454:482	the electrospinning technique using a mixed solvent system	454:511	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	3	39	theme	composite	431:439	arg1	characterization					399:414	the physical and chemical characterization	373:414	the physical and chemical characterization	373:414	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	10	40	theme	materials	1354:1362	arg1	development					1335:1345	development	1335:1345	development	1335:1345	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	10	40	theme	materials	1354:1362	arg1	design					1324:1329	design	1324:1329	design	1324:1329	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	10	41	dep	design	1324:1329	arg1	the					1320:1322	the	1320:1322	the	1320:1322	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	10	42	theme	new	1350:1352	arg1	materials					1354:1362	new materials	1350:1362	new materials based on polylactic acid-chitosan composites for environmental and biomedical applications	1350:1453	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	1	43	theme	novel	135:139	arg1	materials					141:149	Fabricating novel materials	123:149	Fabricating novel materials for biomedical applications	123:177	Fabricating novel materials for biomedical applications mostly require the use of biodegradable materials.
30120580	0	44	theme	bio-based	50:58	arg1	alternative					60:70	a bio-based alternative	48:70	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.	0:121	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	8	45	theme	potential	1121:1129	arg1	measurements					1131:1142	zeta potential measurements	1116:1142	zeta potential measurements	1116:1142	The stability of the composite was established by zeta potential measurements.
30120580	2	46	theme	biodegradable	243:255	arg1	materials					257:265	biodegradable materials	243:265	biodegradable materials like polylactic acid (PLA) and chitosan (CHS)	243:311	In this work biodegradable materials like polylactic acid (PLA) and chitosan (CHS) were used for designing electrospun mats.
30120580	7	47	theme	thermal	1048:1054	arg1	analysis					1056:1063	thermal analysis	1048:1063	thermal analysis	1048:1063	The weak interaction between PLA and chitosan was confirmed by Fourier transform infrared spectroscopy and thermal analysis.
30120580	7	48	theme	weak	945:948	arg1	interaction					950:960	The weak interaction	941:960	The weak interaction between PLA and chitosan	941:985	The weak interaction between PLA and chitosan was confirmed by Fourier transform infrared spectroscopy and thermal analysis.
30120580	0	49	dep	composite	37:45	arg1	alternative					60:70	a bio-based alternative	48:70	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.	0:121	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	9	50	theme	membranes	1173:1181	arg1	studies					1158:1164	Cytotoxicity studies	1145:1164	Cytotoxicity studies of the membranes	1145:1181	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	2	51	theme	electrospun	337:347	arg1	mats					349:352	electrospun mats	337:352	electrospun mats	337:352	In this work biodegradable materials like polylactic acid (PLA) and chitosan (CHS) were used for designing electrospun mats.
30120580	5	52	theme	fiber	683:687	arg1	formation					689:697	The fiber formation	679:697	The fiber formation	679:697	The fiber formation and the reduction in fiber diameter were confirmed by the SEM micrograph.
30120580	4	53	with	fibers	641:646	arg1	optimum					656:662	an optimum	653:662	an optimum at 0.4wt% CHS	653:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	4	54	with	composites	591:600	arg1	uniformity					607:616	uniformity	607:616	uniformity	607:616	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	10	55	theme	acid-chitosan	1384:1396	arg1	composites					1398:1407	polylactic acid-chitosan composites	1373:1407	polylactic acid-chitosan composites for environmental and biomedical applications	1373:1453	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	1	56	theme	biomedical	155:164	arg1	applications					166:177	biomedical applications	155:177	biomedical applications	155:177	Fabricating novel materials for biomedical applications mostly require the use of biodegradable materials.
30120580	2	57	used	used	318:321	arg2	materials					257:265	biodegradable materials	243:265	biodegradable materials like polylactic acid (PLA) and chitosan (CHS)	243:311	In this work biodegradable materials like polylactic acid (PLA) and chitosan (CHS) were used for designing electrospun mats.
30120580	3	58	theme	mixed	492:496	arg1	system					506:511	a mixed solvent system	490:511	a mixed solvent system	490:511	This work reports the physical and chemical characterization of the PLA-CHS composite, prepared by the electrospinning technique using a mixed solvent system.
30120580	0	59	theme	inorganic	76:84	arg1	composites					86:95	inorganic composites	76:95	inorganic composites	76:95	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	6	60	theme	angle	816:820	arg1	measurements					822:833	contact angle measurements	808:833	contact angle measurements	808:833	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	10	61	theme	polylactic	1373:1382	arg1	composites					1398:1407	polylactic acid-chitosan composites	1373:1407	polylactic acid-chitosan composites for environmental and biomedical applications	1373:1453	The current findings are very important for the design and development of new materials based on polylactic acid-chitosan composites for environmental and biomedical applications.
30120580	6	62	theme	concentration	926:938	arg1	increase					907:914	increase	907:914	increase of filler concentration	907:938	The inverse gas chromatography and contact angle measurements supported the increase of hydrophobicity of the composite membrane with increase of filler concentration.
30120580	4	63	from	decrease	557:564	arg1	composites					591:600	the composites	587:600	the composites with uniformity	587:616	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	4	63	from	decrease	557:564	arg1	diameter					575:582	fiber diameter	569:582	fiber diameter	569:582	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	4	63	from	decrease	557:564	arg1	distribution					625:636	the distribution	621:636	the distribution of fibers with an optimum at 0.4wt% CHS	621:676	The addition of chitosan into PLA, offered decrease in fiber diameter in the composites with uniformity in the distribution of fibers with an optimum at 0.4wt% CHS.
30120580	0	64	theme	polylactic	12:21	arg1	composite					37:45	Electrospun polylactic acid-chitosan composite	0:45	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.	0:121	Electrospun polylactic acid-chitosan composite: a bio-based alternative for inorganic composites for advanced application.
30120580	9	65	dep	%	1229:1229	arg1	0.6					1226:1228	0.6	1226:1228	0.6	1226:1228	Cytotoxicity studies of the membranes were also carried out and found that up to 0.6% CHS the composite material was noncytotoxic.
30120580	5	66	theme	fiber	720:724	arg1	diameter					726:733	fiber diameter	720:733	fiber diameter	720:733	The fiber formation and the reduction in fiber diameter were confirmed by the SEM micrograph.
30778562	14	0	theme	PAL	1975:1977	arg1	composites					1979:1988	the PAL composites	1971:1988	the PAL composites improved the antioxidant status of broilers	1971:2032	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	0	theme	PAL	1975:1977	arg1	pronounced					2111:2120	pronounced	2111:2120	pronounced	2111:2120	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	0	theme	PAL	1975:1977	arg1	COS/ZnO/PAL					2064:2074	COS/ZnO/PAL	2064:2074	COS/ZnO/PAL	2064:2074	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	2	1	theme	treatment	325:333	arg1	groups					335:340	3 treatment groups	323:340	3 treatment groups	323:340	A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups.
30778562	14	2	theme	COS/ZnO/PAL	2064:2074	arg1	effects					2053:2059	the beneficial effects	2038:2059	the beneficial effects of COS/ZnO/PAL on the antioxidant status	2038:2100	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	2	theme	COS/ZnO/PAL	2064:2074	arg1	composites					1979:1988	the PAL composites	1971:1988	the PAL composites improved the antioxidant status of broilers	1971:2032	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	2	theme	COS/ZnO/PAL	2064:2074	arg1	pronounced					2111:2120	pronounced	2111:2120	pronounced	2111:2120	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	2	theme	COS/ZnO/PAL	2064:2074	arg1	COS/ZnO/PAL					2064:2074	COS/ZnO/PAL	2064:2074	COS/ZnO/PAL	2064:2074	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	9	3	theme	total	1360:1364	arg1	T-AOC					1388:1392	T-AOC	1388:1392	T-AOC	1388:1392	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	3	theme	total	1360:1364	arg1	capacity					1378:1385	the highest total antioxidant capacity	1348:1385	the highest total antioxidant capacity (T-AOC)	1348:1393	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	4	4	theme	PAL	569:571	arg1	composites					573:582	PAL composites	569:582	PAL composites	569:582	The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05).
30778562	3	5	theme	COS/ZnO/PAL	511:521	arg1	group					523:527	COS/ZnO/PAL group	511:527	COS/ZnO/PAL group	511:527	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	5	theme	COS/ZnO/PAL	511:521	arg1	chitooligosaccharides/ZnO/PAL					480:508	1 g/kg chitooligosaccharides/ZnO/PAL	473:508	1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group)	473:528	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	5	6	theme	P	789:789	arg1	d					786:786	21 and 42 d	776:786	d	786:786	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	6	theme	P	789:789	arg1	<					791:791	P < 0.05	789:796	P < 0.05	789:796	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	9	7	contain	had	1317:1319	arg2	T-AOC					1388:1392	T-AOC	1388:1392	T-AOC	1388:1392	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	7	contain	had	1317:1319	arg1	broilers					1283:1290	broilers	1283:1290	broilers in the COS/ZnO/PAL group	1283:1315	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	7	contain	had	1317:1319	arg2	content					1336:1342	MDA content	1332:1342	MDA content	1332:1342	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	7	contain	had	1317:1319	arg2	capacity					1378:1385	the highest total antioxidant capacity	1348:1385	the highest total antioxidant capacity (T-AOC)	1348:1393	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	10	8	theme	MDA	1469:1471	arg1	content					1473:1479	ileum mucous MDA content	1456:1479	ileum mucous MDA content	1456:1479	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	1	9	theme	composites	170:179	arg1	effects					136:142	the effects	132:142	the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens	132:248	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	7	10	from	activities	1083:1092	arg1	duodenum					1142:1149	the duodenum	1138:1149	the duodenum (P > 0.05)	1138:1160	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	7	10	from	activities	1083:1092	arg1	>					1154:1154	P > 0.05	1152:1159	P > 0.05	1152:1159	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	9	11	dep	content	1336:1342	arg1	the					1321:1323	the	1321:1323	the	1321:1323	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	2	12	theme	Ross	278:281	arg1	broilers					287:294	192 one-day-old Ross 308 broilers	262:294	192 one-day-old Ross 308 broilers	262:294	A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups.
30778562	11	13	dep	groups	1614:1619	arg1	both					1578:1581	both	1578:1581	both	1578:1581	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	8	14	theme	GSH-Px	1205:1210	arg1	highest					1221:1227	highest	1221:1227	highest	1221:1227	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	8	14	theme	GSH-Px	1205:1210	arg1	activities					1183:1192	The duodenal mucous activities	1163:1192	The duodenal mucous activities of SOD and GSH-Px	1163:1210	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	12	15	theme	COS/ZnO/PAL	1720:1730	arg1	groups					1732:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	10	16	theme	CTC	1495:1497	arg1	broilers					1507:1514	CTC treated broilers	1495:1514	CTC treated broilers	1495:1514	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	6	17	theme	serum	850:854	arg1	CAT					866:868	CAT	866:868	CAT	866:868	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	6	17	theme	serum	850:854	arg1	catalase					856:863	serum catalase	850:863	serum catalase (CAT) activity	850:878	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	9	18	theme	P	1411:1411	arg1	jejunum					1402:1408	the jejunum	1398:1408	the jejunum (P < 0.05)	1398:1419	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	18	theme	P	1411:1411	arg1	<					1413:1413	P < 0.05	1411:1418	P < 0.05	1411:1418	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	7	19	theme	P	1152:1152	arg1	duodenum					1142:1149	the duodenum	1138:1149	the duodenum (P > 0.05)	1138:1160	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	7	19	theme	P	1152:1152	arg1	>					1154:1154	P > 0.05	1152:1159	P > 0.05	1152:1159	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	12	20	theme	ZnO/PAL	1708:1714	arg1	groups					1732:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	12	21	from	activities	1685:1694	arg1	groups					1732:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	the ZnO/PAL and COS/ZnO/PAL groups	1704:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	8	22	theme	COS/ZnO/PAL	1236:1246	arg1	group					1248:1252	the COS/ZnO/PAL group	1232:1252	the COS/ZnO/PAL group	1232:1252	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	7	23	theme	dismutase	1108:1116	arg1	activities					1083:1092	activities	1083:1092	activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05)	1083:1160	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	4	24	theme	P	652:652	arg1	>					654:654	P > 0.05	652:659	P > 0.05	652:659	The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05).
30778562	4	24	theme	P	652:652	arg1	CTC					647:649	CTC	647:649	CTC (P > 0.05)	647:660	The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05).
30778562	11	25	theme	ileum	1545:1549	arg1	T-AOC					1558:1562	ileum mucous T-AOC	1545:1562	ileum mucous T-AOC	1545:1562	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	5	26	theme	serum	712:716	arg1	GSH-Px					742:747	GSH-Px	742:747	GSH-Px	742:747	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	26	theme	serum	712:716	arg1	peroxidase					730:739	serum glutathione peroxidase	712:739	serum glutathione peroxidase (GSH-Px)	712:748	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	7	27	theme	CTC	988:990	arg1	group					992:996	the CTC group	984:996	the CTC group	984:996	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	13	28	theme	basal	1832:1836	arg1	diet					1838:1841	the basal diet	1828:1841	the basal diet supplemented with the PAL composites	1828:1878	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	6	29	theme	P	889:889	arg1	<					891:891	P < 0.05	889:896	P < 0.05	889:896	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	12	30	theme	mucous	1671:1676	arg1	activities					1685:1694	The ileum mucous GSH-Px activities	1661:1694	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups	1661:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	5	31	theme	peroxidase	730:739	arg1	activity					700:707	the activity	696:707	the activity of serum glutathione peroxidase (GSH-Px)	696:748	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	32	dep	CTC	764:766	arg1	both					768:771	both	768:771	both	768:771	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	0	33	from	Effects	0:6	arg1	performance					45:55	growth performance	38:55	growth performance	38:55	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	0	33	from	Effects	0:6	arg1	status					73:78	antioxidant status	61:78	antioxidant status	61:78	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	7	34	theme	MDA	1033:1035	arg1	content					1037:1043	duodenal mucous MDA content	1017:1043	duodenal mucous MDA content	1017:1043	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	7	35	theme	mucous	1026:1031	arg1	content					1037:1043	duodenal mucous MDA content	1017:1043	duodenal mucous MDA content	1017:1043	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	1	36	from	effects	136:142	arg1	status					223:228	antioxidant status	211:228	antioxidant status	211:228	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	1	36	from	effects	136:142	arg1	performance					195:205	growth performance	188:205	growth performance	188:205	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	0	37	from	performance	45:55	arg1	chickens					91:98	broiler chickens	83:98	broiler chickens	83:98	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	3	38	theme	CTC	426:428	arg1	chlortetracycline					407:423	either 50 mg/kg chlortetracycline	391:423	either 50 mg/kg chlortetracycline (CTC group)	391:435	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	38	theme	CTC	426:428	arg1	group					430:434	CTC group	426:434	CTC group	426:434	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	7	39	theme	GSH-Px	1128:1133	arg1	activities					1083:1092	activities	1083:1092	activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05)	1083:1160	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	3	40	theme	basal	361:365	arg1	diets					367:371	basal diets	361:371	basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively	361:542	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	6	41	theme	malondialdehyde	920:934	arg1	content					942:948	serum malondialdehyde (MDA) content	914:948	serum malondialdehyde (MDA) content	914:948	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	3	42	theme	1 g/kg	438:443	arg1	group					462:466	ZnO/PAL group	454:466	ZnO/PAL group	454:466	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	42	theme	1 g/kg	438:443	arg1	ZnO/PAL					445:451	1 g/kg ZnO/PAL	438:451	1 g/kg ZnO/PAL (ZnO/PAL group)	438:467	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	0	43	theme	palygorskite	11:22	arg1	composites					24:33	palygorskite composites	11:33	palygorskite composites	11:33	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	10	44	theme	mucous	1462:1467	arg1	content					1473:1479	ileum mucous MDA content	1456:1479	ileum mucous MDA content	1456:1479	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	13	45	theme	growth	1898:1903	arg1	performance					1905:1915	similar growth performance	1890:1915	similar growth performance	1890:1915	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	12	46	theme	P	1783:1783	arg1	group					1776:1780	the CTC group	1768:1780	the CTC group (P < 0.05)	1768:1791	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	12	46	theme	P	1783:1783	arg1	<					1785:1785	P < 0.05	1783:1790	P < 0.05	1783:1790	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	7	47	theme	superoxide	1097:1106	arg1	dismutase					1108:1116	superoxide dismutase	1097:1116	superoxide dismutase (SOD)	1097:1122	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	7	47	theme	superoxide	1097:1106	arg1	SOD					1119:1121	SOD	1119:1121	SOD	1119:1121	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	0	48	theme	antioxidant	61:71	arg1	status					73:78	antioxidant status	61:78	antioxidant status	61:78	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	8	49	theme	mucous	1176:1181	arg1	highest					1221:1227	highest	1221:1227	highest	1221:1227	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	8	49	theme	mucous	1176:1181	arg1	activities					1183:1192	The duodenal mucous activities	1163:1192	The duodenal mucous activities of SOD and GSH-Px	1163:1210	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	14	50	theme	broilers	2025:2032	arg1	status					2015:2020	the antioxidant status	1999:2020	the antioxidant status of broilers	1999:2032	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	11	51	theme	CTC	1639:1641	arg1	group					1643:1647	the CTC group	1635:1647	the CTC group (P < 0.05)	1635:1658	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	11	51	theme	CTC	1639:1641	arg1	<					1652:1652	P < 0.05	1650:1657	P < 0.05	1650:1657	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	9	52	theme	MDA	1332:1334	arg1	content					1336:1342	MDA content	1332:1342	MDA content	1332:1342	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	3	53	theme	1 g/kg	473:478	arg1	group					523:527	COS/ZnO/PAL group	511:527	COS/ZnO/PAL group	511:527	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	53	theme	1 g/kg	473:478	arg1	chitooligosaccharides/ZnO/PAL					480:508	1 g/kg chitooligosaccharides/ZnO/PAL	473:508	1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group)	473:528	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	1	54	theme	PAL	165:167	arg1	composites					170:179	the palygorskite (PAL) composites	147:179	the palygorskite (PAL) composites	147:179	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	14	55	theme	antioxidant	2083:2093	arg1	status					2095:2100	the antioxidant status	2079:2100	the antioxidant status	2079:2100	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	12	56	dep	groups	1732:1737	arg1	both					1696:1699	both	1696:1699	both	1696:1699	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	5	57	theme	glutathione	718:728	arg1	GSH-Px					742:747	GSH-Px	742:747	GSH-Px	742:747	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	57	theme	glutathione	718:728	arg1	peroxidase					730:739	serum glutathione peroxidase	712:739	serum glutathione peroxidase (GSH-Px)	712:748	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	1	58	theme	broiler	233:239	arg1	chickens					241:248	broiler chickens	233:248	broiler chickens	233:248	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	6	59	theme	CTC	818:820	arg1	group					822:826	the CTC group	814:826	the CTC group	814:826	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	9	60	theme	highest	1352:1358	arg1	T-AOC					1388:1392	T-AOC	1388:1392	T-AOC	1388:1392	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	60	theme	highest	1352:1358	arg1	capacity					1378:1385	the highest total antioxidant capacity	1348:1385	the highest total antioxidant capacity (T-AOC)	1348:1393	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	2	61	theme	one-day-old	266:276	arg1	broilers					287:294	192 one-day-old Ross 308 broilers	262:294	192 one-day-old Ross 308 broilers	262:294	A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups.
30778562	9	62	theme	antioxidant	1366:1376	arg1	T-AOC					1388:1392	T-AOC	1388:1392	T-AOC	1388:1392	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	9	62	theme	antioxidant	1366:1376	arg1	capacity					1378:1385	the highest total antioxidant capacity	1348:1385	the highest total antioxidant capacity (T-AOC)	1348:1393	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	10	63	theme	Palygorskite	1422:1433	arg1	composites					1435:1444	Palygorskite composites	1422:1444	Palygorskite composites	1422:1444	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	14	64	theme	beneficial	2042:2051	arg1	effects					2053:2059	the beneficial effects	2038:2059	the beneficial effects of COS/ZnO/PAL on the antioxidant status	2038:2100	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	14	64	theme	beneficial	2042:2051	arg1	pronounced					2111:2120	pronounced	2111:2120	pronounced	2111:2120	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	4	65	theme	growth	625:630	arg1	performance					632:642	growth performance	625:642	growth performance as CTC (P > 0.05)	625:660	The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05).
30778562	11	66	theme	P	1650:1650	arg1	group					1643:1647	the CTC group	1635:1647	the CTC group (P < 0.05)	1635:1658	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	11	66	theme	P	1650:1650	arg1	<					1652:1652	P < 0.05	1650:1657	P < 0.05	1650:1657	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	5	67	theme	21	776:777	arg1	d					786:786	21 and 42 d	776:786	d	786:786	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	67	theme	21	776:777	arg1	<					791:791	P < 0.05	789:796	P < 0.05	789:796	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	2	68	theme	broilers	287:294	arg1	total					253:257	A total	251:257	A total of 192 one-day-old Ross 308 broilers	251:294	A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups.
30778562	6	69	dep	enhanced	841:848	arg1	<					891:891	P < 0.05	889:896	P < 0.05	889:896	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	1	70	theme	palygorskite	151:162	arg1	composites					170:179	the palygorskite (PAL) composites	147:179	the palygorskite (PAL) composites	147:179	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	10	71	theme	treated	1499:1505	arg1	broilers					1507:1514	CTC treated broilers	1495:1514	CTC treated broilers	1495:1514	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	5	72	theme	42	783:784	arg1	d					786:786	21 and 42 d	776:786	d	786:786	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	5	72	theme	42	783:784	arg1	<					791:791	P < 0.05	789:796	P < 0.05	789:796	ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05).
30778562	6	73	theme	catalase	856:863	arg1	activity					871:878	serum catalase (CAT) activity	850:878	serum catalase (CAT) activity	850:878	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	0	74	from	status	73:78	arg1	chickens					91:98	broiler chickens	83:98	broiler chickens	83:98	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	1	75	from	status	223:228	arg1	chickens					241:248	broiler chickens	233:248	broiler chickens	233:248	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	1	76	from	performance	195:205	arg1	chickens					241:248	broiler chickens	233:248	broiler chickens	233:248	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	12	77	theme	GSH-Px	1678:1683	arg1	activities					1685:1694	The ileum mucous GSH-Px activities	1661:1694	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups	1661:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	13	78	theme	PAL	1865:1867	arg1	composites					1869:1878	the PAL composites	1861:1878	the PAL composites	1861:1878	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	2	79	theme	308	283:285	arg1	broilers					287:294	192 one-day-old Ross 308 broilers	262:294	192 one-day-old Ross 308 broilers	262:294	A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups.
30778562	14	80	from	composites	1979:1988	arg1	status					2095:2100	the antioxidant status	2079:2100	the antioxidant status	2079:2100	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	11	81	theme	COS/ZnO/PAL	1602:1612	arg1	groups					1614:1619	the ZnO/PAL and COS/ZnO/PAL groups	1586:1619	the ZnO/PAL and COS/ZnO/PAL groups	1586:1619	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	4	82	theme	similar	606:612	arg1	effects					614:620	similar effects	606:620	similar effects	606:620	The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05).
30778562	9	83	from	broilers	1283:1290	arg1	group					1311:1315	the COS/ZnO/PAL group	1295:1315	the COS/ZnO/PAL group	1295:1315	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	0	84	theme	broiler	83:89	arg1	chickens					91:98	broiler chickens	83:98	broiler chickens	83:98	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	12	85	theme	ileum	1665:1669	arg1	activities					1685:1694	The ileum mucous GSH-Px activities	1661:1694	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups	1661:1737	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	7	86	theme	duodenal	1017:1024	arg1	content					1037:1043	duodenal mucous MDA content	1017:1043	duodenal mucous MDA content	1017:1043	Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05).
30778562	13	87	theme	AGP	1946:1948	arg1	group					1950:1954	the AGP group	1942:1954	the AGP group	1942:1954	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	10	88	theme	ileum	1456:1460	arg1	content					1473:1479	ileum mucous MDA content	1456:1479	ileum mucous MDA content	1456:1479	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	14	89	theme	antioxidant	2003:2013	arg1	status					2015:2020	the antioxidant status	1999:2020	the antioxidant status of broilers	1999:2032	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	13	90	theme	similar	1890:1896	arg1	performance					1905:1915	similar growth performance	1890:1915	similar growth performance	1890:1915	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	6	91	dep	decreased	904:912	arg1	<					961:961	P < 0.05	959:966	P < 0.05	959:966	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	11	92	theme	mucous	1551:1556	arg1	T-AOC					1558:1562	ileum mucous T-AOC	1545:1562	ileum mucous T-AOC	1545:1562	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	8	93	theme	P	1263:1263	arg1	d					1260:1260	42 d	1257:1260	42 d (P < 0.05)	1257:1271	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	8	93	theme	P	1263:1263	arg1	<					1265:1265	P < 0.05	1263:1270	P < 0.05	1263:1270	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	14	94	from	effects	2053:2059	arg1	status					2095:2100	the antioxidant status	2079:2100	the antioxidant status	2079:2100	Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.
30778562	0	95	theme	composites	24:33	arg1	Effects					0:6	Effects	0:6	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens	0:98	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	11	96	theme	ZnO/PAL	1590:1596	arg1	groups					1614:1619	the ZnO/PAL and COS/ZnO/PAL groups	1586:1619	the ZnO/PAL and COS/ZnO/PAL groups	1586:1619	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	6	97	theme	serum	914:918	arg1	MDA					937:939	MDA	937:939	MDA	937:939	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	6	97	theme	serum	914:918	arg1	malondialdehyde					920:934	serum malondialdehyde	914:934	serum malondialdehyde (MDA) content	914:948	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	0	98	theme	growth	38:43	arg1	performance					45:55	growth performance	38:55	growth performance	38:55	Effects of palygorskite composites on growth performance and antioxidant status in broiler chickens.
30778562	1	99	theme	growth	188:193	arg1	performance					195:205	growth performance	188:205	growth performance	188:205	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	9	100	theme	COS/ZnO/PAL	1299:1309	arg1	group					1311:1315	the COS/ZnO/PAL group	1295:1315	the COS/ZnO/PAL group	1295:1315	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	3	101	theme	50 mg/kg	398:405	arg1	chlortetracycline					407:423	either 50 mg/kg chlortetracycline	391:423	either 50 mg/kg chlortetracycline (CTC group)	391:435	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	101	theme	50 mg/kg	398:405	arg1	group					430:434	CTC group	426:434	CTC group	426:434	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	10	102	theme	P	1525:1525	arg1	<					1527:1527	P < 0.05	1525:1532	P < 0.05	1525:1532	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	10	102	theme	P	1525:1525	arg1	d					1522:1522	21 d	1519:1522	21 d (P < 0.05)	1519:1533	Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05).
30778562	8	103	theme	duodenal	1167:1174	arg1	highest					1221:1227	highest	1221:1227	highest	1221:1227	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	8	103	theme	duodenal	1167:1174	arg1	activities					1183:1192	The duodenal mucous activities	1163:1192	The duodenal mucous activities of SOD and GSH-Px	1163:1210	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	12	104	theme	CTC	1772:1774	arg1	group					1776:1780	the CTC group	1768:1780	the CTC group (P < 0.05)	1768:1791	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	12	104	theme	CTC	1772:1774	arg1	<					1785:1785	P < 0.05	1783:1790	P < 0.05	1783:1790	The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05).
30778562	9	105	theme	lowest	1325:1330	arg1	content					1336:1342	MDA content	1332:1342	MDA content	1332:1342	At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05).
30778562	6	106	theme	P	959:959	arg1	<					961:961	P < 0.05	959:966	P < 0.05	959:966	Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05).
30778562	1	107	dep	performance	195:205	arg1	the					184:186	the	184:186	the	184:186	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	13	108	from	counterparts	1926:1937	arg1	group					1950:1954	the AGP group	1942:1954	the AGP group	1942:1954	In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group.
30778562	3	109	theme	ZnO/PAL	454:460	arg1	group					462:466	ZnO/PAL group	454:466	ZnO/PAL group	454:466	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	3	109	theme	ZnO/PAL	454:460	arg1	ZnO/PAL					445:451	1 g/kg ZnO/PAL	438:451	1 g/kg ZnO/PAL (ZnO/PAL group)	438:467	Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively.
30778562	1	110	theme	antioxidant	211:221	arg1	status					223:228	antioxidant status	211:228	antioxidant status	211:228	This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens.
30778562	11	111	dep	increased	1568:1576	arg1	compared					1621:1628	compared	1621:1628	compared with the CTC group (P < 0.05)	1621:1658	At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05).
30778562	8	112	theme	SOD	1197:1199	arg1	highest					1221:1227	highest	1221:1227	highest	1221:1227	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
30778562	8	112	theme	SOD	1197:1199	arg1	activities					1183:1192	The duodenal mucous activities	1163:1192	The duodenal mucous activities of SOD and GSH-Px	1163:1210	The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05).
29437169	7	0	dep	in	1062:1063	arg1	vitro					1065:1069	vitro	1065:1069	vitro	1065:1069	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	11	1	theme	diverse	1624:1630	arg1	fractions					1632:1640	diverse fractions	1624:1640	diverse fractions with different prebiotic properties starting from the same biomass	1624:1707	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	7	2	theme	bacteria	1114:1121	arg1	stimulation					1129:1139	lactic acid bacteria (LAB) stimulation	1102:1139	lactic acid bacteria (LAB) stimulation by POS fractions	1102:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	3	3	theme	pectin	490:495	arg1	hydrolysis					505:514	the hydrolysis	501:514	the hydrolysis of pectin	501:524	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	3	3	theme	pectin	490:495	arg1	extraction					476:485	the extraction	472:485	the extraction of pectin	472:495	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	1	4	theme	food	245:248	arg1	byproducts					250:259	pectin-rich food byproducts	233:259	pectin-rich food byproducts	233:259	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	4	5	theme	Different	591:599	arg1	extraction					601:610	Different extraction	591:610	Different extraction as well as hydrolysis and fractionation methodologies	591:664	Different extraction as well as hydrolysis and fractionation methodologies were applied.
29437169	7	6	theme	lactic	1102:1107	arg1	LAB					1124:1126	LAB	1124:1126	LAB	1124:1126	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	7	6	theme	lactic	1102:1107	arg1	bacteria					1114:1121	lactic acid bacteria	1102:1121	lactic acid bacteria (LAB) stimulation by POS fractions	1102:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	2	7	theme	present	269:275	arg1	study					277:281	the present study	265:281	the present study	265:281	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	9	8	theme	subsequent	1404:1413	arg1	fractionation					1415:1427	subsequent fractionation	1404:1427	subsequent fractionation	1404:1427	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	8	9	from	stimulation	1200:1210	arg1	efficient					1187:1195	efficient	1187:1195	efficient	1187:1195	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	11	10	theme	prebiotic	1657:1665	arg1	properties					1667:1676	different prebiotic properties	1647:1676	different prebiotic properties starting from the same biomass	1647:1707	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	8	11	from	efficient	1187:1195	arg1	manner					1236:1241	a species-dependent manner	1216:1241	a species-dependent manner	1216:1241	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	8	11	from	efficient	1187:1195	arg1	stimulation					1200:1210	stimulation	1200:1210	stimulation	1200:1210	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	2	12	theme	technological	294:306	arg1	means					308:312	different technological means	284:312	different technological means of POS production	284:330	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	9	13	theme	molecular	1445:1453	arg1	weight					1455:1460	low-medium molecular weight	1434:1460	low-medium molecular weight	1434:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	7	14	theme	in	1062:1063	arg1	experiments					1071:1081	several in vitro experiments	1054:1081	several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions	1054:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	1	15	theme	compounds	195:203	arg1	class					166:170	a new class	160:170	a new class	160:170	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	1	15	theme	compounds	195:203	arg1	oligosaccharides					114:129	Pectin oligosaccharides	107:129	Pectin oligosaccharides (POS)	107:135	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	11	16	theme	prebiotic	1818:1826	arg1	compounds					1828:1836	prebiotic compounds	1818:1836	prebiotic compounds	1818:1836	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	5	17	theme	galacturonic	732:743	arg1	content					750:756	their total galacturonic acid content	720:756	their total galacturonic acid content	720:756	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	8	18	from	manner	1236:1241	arg1	efficient					1187:1195	efficient	1187:1195	efficient	1187:1195	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	10	19	dep	E.	1541:1542	arg1	coli					1544:1547	coli	1544:1547	coli	1544:1547	Quite interestingly, no POS fraction was able to stimulate pathogenic E. coli strains.
29437169	9	20	theme	overall	1248:1254	arg1	cut-off					1462:1468	cut-off	1462:1468	cut-off	1462:1468	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	20	theme	overall	1248:1254	arg1	fractions					1261:1269	The overall best fractions	1244:1269	The overall best fractions	1244:1269	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	7	21	theme	potential	966:974	arg1	properties					986:995	the potential prebiotic properties	962:995	the potential prebiotic properties of the POS mixtures obtained	962:1024	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	0	22	theme	potential	77:85	arg1	activity					97:104	potential prebiotic activity	77:104	potential prebiotic activity	77:104	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	7	23	dep	fractions	1148:1156	arg1	POS					1144:1146	POS fractions	1144:1156	POS fractions	1144:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	11	24	theme	sugar	1760:1764	arg1	pulp					1771:1774	sugar beet pulp	1760:1774	sugar beet pulp	1760:1774	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	11	25	theme	different	1647:1655	arg1	properties					1667:1676	different prebiotic properties	1647:1676	different prebiotic properties starting from the same biomass	1647:1707	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	0	26	theme	Pectin	0:5	arg1	oligosaccharides					7:22	Pectin oligosaccharides	0:22	Pectin oligosaccharides from sugar beet pulp	0:43	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	9	27	dep	general	1279:1285	arg1	cut-off					1462:1468	cut-off	1462:1468	cut-off	1462:1468	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	27	dep	general	1279:1285	arg1	fractions					1261:1269	The overall best fractions	1244:1269	The overall best fractions	1244:1269	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	10	28	theme	POS	1495:1497	arg1	fraction					1499:1506	no POS fraction	1492:1506	no POS fraction	1492:1506	Quite interestingly, no POS fraction was able to stimulate pathogenic E. coli strains.
29437169	9	29	theme	low	1320:1322	arg1	degree					1324:1329	a low degree	1318:1329	a low degree	1318:1329	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	30	theme	biomass	1392:1398	arg1	fractionation					1415:1427	subsequent fractionation	1404:1427	subsequent fractionation	1404:1427	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	30	theme	biomass	1392:1398	arg1	extraction					1378:1387	the enzymatic extraction	1364:1387	the enzymatic extraction of biomass	1364:1398	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	0	31	dep	characterization	56:71	arg1	oligosaccharides					7:22	Pectin oligosaccharides	0:22	Pectin oligosaccharides from sugar beet pulp	0:43	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	2	32	theme	beet	402:405	arg1	pulp					407:410	sugar beet pulp	396:410	sugar beet pulp	396:410	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	0	33	theme	beet	35:38	arg1	pulp					40:43	sugar beet pulp	29:43	sugar beet pulp	29:43	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	11	34	theme	same	1696:1699	arg1	biomass					1701:1707	the same biomass	1692:1707	the same biomass	1692:1707	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	5	35	theme	HILIC-ESI/MS	790:801	arg1	methodology					803:813	a HILIC-ESI/MS methodology	788:813	a HILIC-ESI/MS methodology	788:813	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	4	36	dep	hydrolysis	623:632	arg1	methodologies					652:664	methodologies	652:664	methodologies	652:664	Different extraction as well as hydrolysis and fractionation methodologies were applied.
29437169	9	37	with	fractionation	1415:1427	arg1	weight					1455:1460	low-medium molecular weight	1434:1460	low-medium molecular weight	1434:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	2	38	dep	mixtures	373:380	arg1	POS					369:371	tailor-made POS mixtures	357:380	tailor-made POS mixtures starting from sugar beet pulp	357:410	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	9	39	contain	having	1311:1316	arg2	degree					1324:1329	a low degree	1318:1329	a low degree	1318:1329	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	39	contain	having	1311:1316	arg1	arabinans					1301:1309	arabinans	1301:1309	arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight	1301:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	3	40	dep	consisted	448:456	arg1	hydrolysis					505:514	the hydrolysis	501:514	the hydrolysis of pectin	501:524	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	3	40	dep	consisted	448:456	arg1	extraction					476:485	the extraction	472:485	the extraction of pectin	472:495	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	9	41	with	extraction	1378:1387	arg1	weight					1455:1460	low-medium molecular weight	1434:1460	low-medium molecular weight	1434:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	2	42	theme	tailor-made	357:367	arg1	mixtures					373:380	tailor-made POS mixtures	357:380	tailor-made POS mixtures starting from sugar beet pulp	357:410	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	9	43	theme	enzymatic	1368:1376	arg1	extraction					1378:1387	the enzymatic extraction	1364:1387	the enzymatic extraction of biomass	1364:1398	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	10	44	theme	pathogenic	1530:1539	arg1	strains					1549:1555	pathogenic E. coli strains	1530:1555	pathogenic E. coli strains	1530:1555	Quite interestingly, no POS fraction was able to stimulate pathogenic E. coli strains.
29437169	5	45	theme	deeper	768:773	arg1	level					775:779	a deeper level	766:779	a deeper level	766:779	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	7	46	theme	acid	1109:1112	arg1	LAB					1124:1126	LAB	1124:1126	LAB	1124:1126	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	7	46	theme	acid	1109:1112	arg1	bacteria					1114:1121	lactic acid bacteria	1102:1121	lactic acid bacteria (LAB) stimulation by POS fractions	1102:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	11	47	with	fractions	1632:1640	arg1	properties					1667:1676	different prebiotic properties	1647:1676	different prebiotic properties starting from the same biomass	1647:1707	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	1	48	theme	pectin-rich	233:243	arg1	byproducts					250:259	pectin-rich food byproducts	233:259	pectin-rich food byproducts	233:259	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	3	49	theme	pectin	519:524	arg1	hydrolysis					505:514	the hydrolysis	501:514	the hydrolysis of pectin	501:524	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	3	49	theme	pectin	519:524	arg1	extraction					476:485	the extraction	472:485	the extraction of pectin	472:495	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	1	50	theme	new	162:164	arg1	class					166:170	a new class	160:170	a new class	160:170	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	1	50	theme	new	162:164	arg1	oligosaccharides					114:129	Pectin oligosaccharides	107:129	Pectin oligosaccharides (POS)	107:135	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	6	51	theme	fractions	878:886	arg1	function					905:912	a function	903:912	a function of the technology used to obtain them	903:950	The composition of POS fractions was studied as a function of the technology used to obtain them.
29437169	6	51	theme	fractions	878:886	arg1	composition					859:869	The composition	855:869	The composition of POS fractions	855:886	The composition of POS fractions was studied as a function of the technology used to obtain them.
29437169	9	52	from	arabinans	1301:1309	arg1	rich					1293:1296	rich	1293:1296	rich	1293:1296	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	53	theme	low-medium	1434:1443	arg1	weight					1455:1460	low-medium molecular weight	1434:1460	low-medium molecular weight	1434:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	8	54	theme	Several	1159:1165	arg1	fractions					1167:1175	Several fractions	1159:1175	Several fractions	1159:1175	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	8	55	theme	species-dependent	1218:1234	arg1	manner					1236:1241	a species-dependent manner	1216:1241	a species-dependent manner	1216:1241	Several fractions were very efficient in stimulation, in a species-dependent manner.
29437169	7	56	theme	several	1054:1060	arg1	experiments					1071:1081	several in vitro experiments	1054:1081	several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions	1054:1156	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	3	57	theme	tailored	529:536	arg1	POS					538:540	tailored POS	529:540	tailored POS	529:540	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	1	58	theme	prebiotic	185:193	arg1	compounds					195:203	potential prebiotic compounds	175:203	potential prebiotic compounds	175:203	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	3	59	theme	combined	545:552	arg1	approaches					579:588	combined hydrolysis/fractionation approaches	545:588	combined hydrolysis/fractionation approaches	545:588	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	11	60	theme	appropriate	1785:1795	arg1	technology					1797:1806	the appropriate technology	1781:1806	the appropriate technology	1781:1806	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	5	61	theme	total	726:730	arg1	content					750:756	their total galacturonic acid content	720:756	their total galacturonic acid content	720:756	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	1	62	theme	potential	175:183	arg1	compounds					195:203	potential prebiotic compounds	175:203	potential prebiotic compounds	175:203	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	5	63	theme	obtained	684:691	arg1	POS					693:695	The obtained POS	680:695	The obtained POS	680:695	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	3	64	theme	hydrolysis/fractionation	554:577	arg1	approaches					579:588	combined hydrolysis/fractionation approaches	545:588	combined hydrolysis/fractionation approaches	545:588	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	7	65	theme	POS	1004:1006	arg1	mixtures					1008:1015	the POS mixtures	1000:1015	the POS mixtures obtained	1000:1024	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	6	66	theme	technology	921:930	arg1	composition					859:869	The composition	855:869	The composition of POS fractions	855:886	The composition of POS fractions was studied as a function of the technology used to obtain them.
29437169	6	66	theme	technology	921:930	arg1	function					905:912	a function	903:912	a function of the technology used to obtain them	903:950	The composition of POS fractions was studied as a function of the technology used to obtain them.
29437169	7	67	theme	mixtures	1008:1015	arg1	properties					986:995	the potential prebiotic properties	962:995	the potential prebiotic properties of the POS mixtures obtained	962:1024	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	2	68	theme	different	284:292	arg1	means					308:312	different technological means	284:312	different technological means of POS production	284:330	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	9	69	theme	best	1256:1259	arg1	cut-off					1462:1468	cut-off	1462:1468	cut-off	1462:1468	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	9	69	theme	best	1256:1259	arg1	fractions					1261:1269	The overall best fractions	1244:1269	The overall best fractions	1244:1269	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	7	70	theme	prebiotic	976:984	arg1	properties					986:995	the potential prebiotic properties	962:995	the potential prebiotic properties of the POS mixtures obtained	962:1024	Finally, the potential prebiotic properties of the POS mixtures obtained were thoroughly explored by several in vitro experiments aimed at detecting lactic acid bacteria (LAB) stimulation by POS fractions.
29437169	9	71	from	rich	1293:1296	arg1	arabinans					1301:1309	arabinans	1301:1309	arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight	1301:1460	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	0	72	theme	prebiotic	87:95	arg1	activity					97:104	potential prebiotic activity	77:104	potential prebiotic activity	77:104	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	5	73	theme	acid	745:748	arg1	content					750:756	their total galacturonic acid content	720:756	their total galacturonic acid content	720:756	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
29437169	11	74	theme	beet	1766:1769	arg1	pulp					1771:1774	sugar beet pulp	1760:1774	sugar beet pulp	1760:1774	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	0	75	from	pulp	40:43	arg1	oligosaccharides					7:22	Pectin oligosaccharides	0:22	Pectin oligosaccharides from sugar beet pulp	0:43	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	3	76	theme	production	429:438	arg1	process					440:446	The overall POS production process	413:446	The overall POS production process	413:446	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	3	77	theme	overall	417:423	arg1	process					440:446	The overall POS production process	413:446	The overall POS production process	413:446	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	0	78	theme	sugar	29:33	arg1	pulp					40:43	sugar beet pulp	29:43	sugar beet pulp	29:43	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	1	79	theme	Pectin	107:112	arg1	class					166:170	a new class	160:170	a new class	160:170	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	1	79	theme	Pectin	107:112	arg1	POS					132:134	POS	132:134	POS	132:134	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	1	79	theme	Pectin	107:112	arg1	oligosaccharides					114:129	Pectin oligosaccharides	107:129	Pectin oligosaccharides (POS)	107:135	Pectin oligosaccharides (POS) have been indicated as a new class of potential prebiotic compounds, which can be produced from pectin-rich food byproducts.
29437169	2	80	dep	production	321:330	arg1	POS					317:319	POS production	317:330	POS production	317:330	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	2	81	theme	sugar	396:400	arg1	pulp					407:410	sugar beet pulp	396:410	sugar beet pulp	396:410	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	0	82	theme	molecular	46:54	arg1	characterization					56:71	molecular characterization	46:71	molecular characterization	46:71	Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.
29437169	9	83	theme	polymerization	1334:1347	arg1	degree					1324:1329	a low degree	1318:1329	a low degree	1318:1329	The overall best fractions were in general those rich in arabinans having a low degree of polymerization, obtained from the enzymatic extraction of biomass and subsequent fractionation with low-medium molecular weight cut-off.
29437169	11	84	theme	clear	1722:1726	arg1	potential					1728:1736	clear potential	1722:1736	clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds	1722:1836	The data reported here clearly indicate the possibility to obtain diverse fractions with different prebiotic properties starting from the same biomass, and outline clear potential for POS obtained from sugar beet pulp with the appropriate technology to act as prebiotic compounds.
29437169	2	85	theme	production	321:330	arg1	means					308:312	different technological means	284:312	different technological means of POS production	284:330	In the present study, different technological means of POS production were explored to produce tailor-made POS mixtures starting from sugar beet pulp.
29437169	3	86	dep	process	440:446	arg1	POS					425:427	The overall POS production process	413:446	The overall POS production process	413:446	The overall POS production process consisted of two steps: the extraction of pectin and the hydrolysis of pectin to tailored POS by combined hydrolysis/fractionation approaches.
29437169	6	87	dep	fractions	878:886	arg1	POS					874:876	POS fractions	874:886	POS fractions	874:886	The composition of POS fractions was studied as a function of the technology used to obtain them.
29437169	10	88	theme	E.	1541:1542	arg1	strains					1549:1555	pathogenic E. coli strains	1530:1555	pathogenic E. coli strains	1530:1555	Quite interestingly, no POS fraction was able to stimulate pathogenic E. coli strains.
29437169	5	89	dep	structure	828:836	arg1	POS					824:826	the POS structure	820:836	the POS structure	820:836	The obtained POS were characterized for their total galacturonic acid content and, at a deeper level, using a HILIC-ESI/MS methodology, for the POS structure and composition.
30948070	7	0	theme	MG63	1243:1246	arg1	cells					1248:1252	MG63 cells	1243:1252	MG63 cells	1243:1252	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	3	1	theme	precipitation	598:610	arg1	method					612:617	homogenous precipitation method	587:617	homogenous precipitation method	587:617	The fibers were synthesized by homogenous precipitation method and were characterized.
30948070	5	2	theme	high	927:930	arg1	porosity					932:939	high porosity	927:939	high porosity (61-75%)	927:948	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	5	2	theme	high	927:930	arg1	%					947:947	61-75%	942:947	61-75%	942:947	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	0	3	theme	whisker-like	150:161	arg1	fibers					163:168	multiphasic calcium phosphate whisker-like fibers	120:168	multiphasic calcium phosphate whisker-like fibers for bone tissue engineering	120:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	5	4	theme	composite	866:874	arg1	scaffolds					876:884	The composite scaffolds	862:884	The composite scaffolds	862:884	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	8	5	theme	cell	1429:1432	arg1	viability					1434:1442	higher cell viability	1422:1442	higher cell viability	1422:1442	Triphasic fibers were more effective in reinforcing the scaffolds and resulted in higher cell viability.
30948070	4	6	theme	hydroxyapatite	683:696	arg1	phases					673:678	two phases	669:678	two phases of hydroxyapatite (HA) and monetite	669:714	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	10	7	theme	potential	1782:1790	arg1	scaffolds					1768:1776	the developed composite scaffolds	1744:1776	the developed composite scaffolds	1744:1776	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	10	7	theme	potential	1782:1790	arg1	candidates					1792:1801	potential candidates	1782:1801	potential candidates for bone tissue engineering	1782:1829	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	0	8	theme	tissue	179:184	arg1	engineering					186:196	bone tissue engineering	174:196	bone tissue engineering	174:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	9	9	theme	CS	1703:1704	arg1	scaffolds					1706:1714	pure CS scaffolds	1698:1714	pure CS scaffolds	1698:1714	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	4	10	contain	contained	659:667	arg1	fibers					652:657	Biphasic fibers	643:657	Biphasic fibers	643:657	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	4	10	contain	contained	659:667	arg2	phases					673:678	two phases	669:678	two phases of hydroxyapatite (HA) and monetite	669:714	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	7	11	theme	red	1326:1328	arg1	staining					1330:1337	Alizarin red staining	1317:1337	Alizarin red staining	1317:1337	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	9	12	theme	triphasic	1502:1510	arg1	fibers					1512:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	13	from	terms	1536:1540	arg1	superior					1524:1531	superior	1524:1531	superior	1524:1531	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	10	14	from	enhancement	1858:1868	arg1	properties					1884:1893	mechanical properties	1873:1893	mechanical properties	1873:1893	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	4	15	theme	triphasic	721:729	arg1	fibers					731:736	triphasic fibers	721:736	triphasic fibers	721:736	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	0	16	theme	cellulose	94:102	arg1	properties					57:66	physicochemical, mechanical and biological properties	14:66	physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose	14:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	9	17	from	superior	1524:1531	arg1	terms					1536:1540	terms	1536:1540	terms of mechanical and biological properties	1536:1580	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	10	18	theme	tissue	1812:1817	arg1	engineering					1819:1829	bone tissue engineering	1807:1829	bone tissue engineering	1807:1829	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	5	19	theme	35-200 μm	987:995	arg1	range					978:982	range	978:982	range of 35-200 μm	978:995	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	1	20	theme	drying	384:389	arg1	method					391:396	freeze drying method	377:396	freeze drying method	377:396	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	2	21	theme	scaffolds	531:539	arg1	properties					503:512	the mechanical, physicochemical and biological properties	456:512	the mechanical, physicochemical and biological properties of the composite scaffolds	456:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	8	22	theme	Triphasic	1340:1348	arg1	fibers					1350:1355	Triphasic fibers	1340:1355	Triphasic fibers	1340:1355	Triphasic fibers were more effective in reinforcing the scaffolds and resulted in higher cell viability.
30948070	0	23	theme	calcium	132:138	arg1	phosphate					140:148	multiphasic calcium phosphate	120:148	multiphasic calcium phosphate whisker-like fibers for bone tissue engineering	120:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	7	24	theme	Alizarin	1317:1324	arg1	staining					1330:1337	Alizarin red staining	1317:1337	Alizarin red staining	1317:1337	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	4	25	theme	β-tricalcium	755:766	arg1	phosphate					768:776	β-tricalcium phosphate	755:776	β-tricalcium phosphate	755:776	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	4	25	theme	β-tricalcium	755:766	arg1	HA					751:752	HA	751:752	HA	751:752	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	2	26	theme	content	445:451	arg1	addition					413:420	addition	413:420	addition of CMC, fiber type and content	413:451	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	2	27	theme	type	436:439	arg1	addition					413:420	addition	413:420	addition of CMC, fiber type and content	413:451	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	0	28	theme	physicochemical	14:28	arg1	properties					57:66	physicochemical, mechanical and biological properties	14:66	physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose	14:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	29	theme	biphasic	310:317	arg1	fibers					351:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	9	30	theme	properties	1571:1580	arg1	terms					1536:1540	terms	1536:1540	terms of mechanical and biological properties	1536:1580	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	0	31	theme	mechanical	31:40	arg1	properties					57:66	physicochemical, mechanical and biological properties	14:66	physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose	14:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	32	theme	triphasic	323:331	arg1	fibers					351:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	7	33	dep	in	1186:1187	arg1	vitro					1189:1193	vitro	1189:1193	vitro	1189:1193	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	0	34	theme	biological	46:55	arg1	properties					57:66	physicochemical, mechanical and biological properties	14:66	physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose	14:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	35	theme	phosphate	341:349	arg1	fibers					351:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	7	36	theme	MTT	1283:1285	arg1	assay					1287:1291	MTT assay	1283:1291	MTT assay	1283:1291	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	10	37	theme	mechanical	1873:1882	arg1	properties					1884:1893	mechanical properties	1873:1893	mechanical properties	1873:1893	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	8	38	theme	higher	1422:1427	arg1	viability					1434:1442	higher cell viability	1422:1442	higher cell viability	1422:1442	Triphasic fibers were more effective in reinforcing the scaffolds and resulted in higher cell viability.
30948070	1	39	theme	carboxymethyl	251:263	arg1	CMC					276:278	CMC	276:278	CMC	276:278	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	1	39	theme	carboxymethyl	251:263	arg1	cellulose					265:273	carboxymethyl cellulose	251:273	carboxymethyl cellulose (CMC)	251:279	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	7	40	theme	in	1186:1187	arg1	proliferation					1195:1207	the in vitro proliferation	1182:1207	the in vitro proliferation	1182:1207	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	9	41	theme	150 kPa	1629:1635	arg1	modulus					1618:1624	modulus	1618:1624	modulus	1618:1624	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	41	theme	150 kPa	1629:1635	arg1	strength					1605:1612	compressive strength	1593:1612	compressive strength	1593:1612	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	42	theme	3.08 MPa	1641:1648	arg1	modulus					1618:1624	modulus	1618:1624	modulus	1618:1624	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	42	theme	3.08 MPa	1641:1648	arg1	strength					1605:1612	compressive strength	1593:1612	compressive strength	1593:1612	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	7	43	theme	cells	1248:1252	arg1	proliferation					1195:1207	the in vitro proliferation	1182:1207	the in vitro proliferation	1182:1207	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	7	43	theme	cells	1248:1252	arg1	mineralization					1225:1238	mineralization	1225:1238	mineralization	1225:1238	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	7	43	theme	cells	1248:1252	arg1	attachment					1210:1219	attachment	1210:1219	attachment	1210:1219	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	5	44	from	microstructures	906:920	arg1	range					978:982	range	978:982	range of 35-200 μm	978:995	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	10	45	theme	composite	1758:1766	arg1	scaffolds					1768:1776	the developed composite scaffolds	1744:1776	the developed composite scaffolds	1744:1776	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	10	45	theme	composite	1758:1766	arg1	candidates					1792:1801	potential candidates	1782:1801	potential candidates for bone tissue engineering	1782:1829	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	5	46	theme	desirable	896:904	arg1	microstructures					906:920	desirable microstructures	896:920	desirable microstructures with high porosity (61-75%)	896:948	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	9	47	dep	300	1680:1682	arg1	to					1677:1678	to	1677:1678	to	1677:1678	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	3	48	theme	homogenous	587:596	arg1	method					612:617	homogenous precipitation method	587:617	homogenous precipitation method	587:617	The fibers were synthesized by homogenous precipitation method and were characterized.
30948070	1	49	theme	porous	213:218	arg1	scaffolds					220:228	porous scaffolds	213:228	porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC)	213:279	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	6	50	theme	CMC	1010:1012	arg1	Addition					998:1005	Addition	998:1005	Addition of CMC to CS	998:1018	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	10	51	theme	developed	1748:1756	arg1	scaffolds					1768:1776	the developed composite scaffolds	1744:1776	the developed composite scaffolds	1744:1776	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	10	51	theme	developed	1748:1756	arg1	candidates					1792:1801	potential candidates	1782:1801	potential candidates for bone tissue engineering	1782:1829	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	6	52	theme	water	1118:1122	arg1	uptake					1124:1129	the water uptake ability and biocompatibility	1114:1158	uptake	1124:1129	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	0	53	theme	bone	174:177	arg1	engineering					186:196	bone tissue engineering	174:196	bone tissue engineering	174:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	9	54	theme	Composite	1445:1453	arg1	scaffolds					1455:1463	Composite scaffolds	1445:1463	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers	1445:1517	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	55	theme	CS	1468:1469	arg1	fibers					1512:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	56	theme	greater	1685:1691	arg1	modulus					1618:1624	modulus	1618:1624	modulus	1618:1624	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	56	theme	greater	1685:1691	arg1	strength					1605:1612	compressive strength	1593:1612	compressive strength	1593:1612	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	57	theme	CMC	1475:1477	arg1	fibers					1512:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	CS and CMC reinforced with 50 wt% triphasic fibers	1468:1517	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	9	58	theme	pure	1698:1701	arg1	scaffolds					1706:1714	pure CS scaffolds	1698:1714	pure CS scaffolds	1698:1714	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	5	59	theme	interconnected	954:967	arg1	pores					969:973	interconnected pores	954:973	interconnected pores in range of 35-200 μm	954:995	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	0	60	theme	chitosan/carboxymethyl	71:92	arg1	cellulose					94:102	chitosan/carboxymethyl cellulose	71:102	chitosan/carboxymethyl cellulose	71:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	10	61	theme	bone	1807:1810	arg1	engineering					1819:1829	bone tissue engineering	1807:1829	bone tissue engineering	1807:1829	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	4	62	from	20-145 μm	827:835	arg1	length					840:845	length	840:845	length	840:845	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	6	63	theme	significant	1029:1039	arg1	improvement					1041:1051	a significant improvement	1027:1051	a significant improvement in the mechanical properties (up to 150%)	1027:1093	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	4	64	theme	monetite	707:714	arg1	phases					673:678	two phases	669:678	two phases of hydroxyapatite (HA) and monetite	669:714	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	4	65	from	20-270 μm	813:821	arg1	length					840:845	length	840:845	length	840:845	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	9	66	theme	fibers	1512:1517	arg1	scaffolds					1455:1463	Composite scaffolds	1445:1463	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers	1445:1517	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	2	67	theme	biological	492:501	arg1	properties					503:512	the mechanical, physicochemical and biological properties	456:512	the mechanical, physicochemical and biological properties of the composite scaffolds	456:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	0	68	theme	multiphasic	120:130	arg1	phosphate					140:148	multiphasic calcium phosphate	120:148	multiphasic calcium phosphate whisker-like fibers for bone tissue engineering	120:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	69	theme	freeze	377:382	arg1	method					391:396	freeze drying method	377:396	freeze drying method	377:396	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	6	70	dep	uptake	1124:1129	arg1	ability					1131:1137	ability	1131:1137	ability	1131:1137	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	6	71	from	improvement	1041:1051	arg1	properties					1071:1080	the mechanical properties	1056:1080	the mechanical properties (up to 150%)	1056:1093	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	6	71	from	improvement	1041:1051	arg1	%					1092:1092	up to 150%	1083:1092	up to 150%	1083:1092	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	4	72	theme	calcium	782:788	arg1	pyrophosphate					790:802	calcium pyrophosphate	782:802	calcium pyrophosphate	782:802	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	4	72	theme	calcium	782:788	arg1	HA					751:752	HA	751:752	HA	751:752	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	0	73	theme	phosphate	140:148	arg1	fibers					163:168	multiphasic calcium phosphate whisker-like fibers	120:168	multiphasic calcium phosphate whisker-like fibers for bone tissue engineering	120:196	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	74	theme	chitosan	233:240	arg1	scaffolds					220:228	porous scaffolds	213:228	porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC)	213:279	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	2	75	theme	composite	521:529	arg1	scaffolds					531:539	the composite scaffolds	517:539	the composite scaffolds	517:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	5	76	with	pores	969:973	arg1	porosity					932:939	high porosity	927:939	high porosity (61-75%)	927:948	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	5	76	with	pores	969:973	arg1	%					947:947	61-75%	942:947	61-75%	942:947	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	2	77	theme	addition	413:420	arg1	effect					403:408	The effect	399:408	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds	399:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	5	78	from	pores	969:973	arg1	range					978:982	range	978:982	range of 35-200 μm	978:995	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	9	79	theme	mechanical	1545:1554	arg1	properties					1571:1580	mechanical and biological properties	1545:1580	mechanical and biological properties	1545:1580	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	1	80	theme	whisker-like	297:308	arg1	fibers					351:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	9	81	theme	biological	1560:1569	arg1	properties					1571:1580	mechanical and biological properties	1545:1580	mechanical and biological properties	1545:1580	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	7	82	theme	DAPI	1294:1297	arg1	staining					1299:1306	DAPI staining	1294:1306	DAPI staining	1294:1306	Both fibers improved the in vitro proliferation, attachment and mineralization of MG63 cells on scaffolds as evidenced by MTT assay, DAPI staining, SEM and Alizarin red staining.
30948070	10	83	theme	further	1850:1856	arg1	enhancement					1858:1868	further enhancement	1850:1868	further enhancement in mechanical properties	1850:1893	The findings indicate that the developed composite scaffolds are potential candidates for bone tissue engineering although they need further enhancement in mechanical properties.
30948070	6	84	dep	150	1089:1091	arg1	to					1086:1087	to	1086:1087	to	1086:1087	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	2	85	theme	fiber	430:434	arg1	type					436:439	fiber type	430:439	fiber type	430:439	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	6	86	theme	mechanical	1060:1069	arg1	properties					1071:1080	the mechanical properties	1056:1080	the mechanical properties (up to 150%)	1056:1093	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	6	86	theme	mechanical	1060:1069	arg1	%					1092:1092	up to 150%	1083:1092	up to 150%	1083:1092	Addition of CMC to CS led to a significant improvement in the mechanical properties (up to 150%) but did not affect the water uptake ability and biocompatibility.
30948070	2	87	theme	physicochemical	472:486	arg1	properties					503:512	the mechanical, physicochemical and biological properties	456:512	the mechanical, physicochemical and biological properties of the composite scaffolds	456:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	2	88	theme	CMC	425:427	arg1	addition					413:420	addition	413:420	addition of CMC, fiber type and content	413:451	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	0	89	theme	properties	57:66	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose	0:102	Evaluation of physicochemical, mechanical and biological properties of chitosan/carboxymethyl cellulose reinforced with multiphasic calcium phosphate whisker-like fibers for bone tissue engineering.
30948070	1	90	theme	calcium	333:339	arg1	fibers					351:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	whisker-like biphasic and triphasic calcium phosphate fibers	297:356	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30948070	9	91	theme	compressive	1593:1603	arg1	strength					1605:1612	compressive strength	1593:1612	compressive strength	1593:1612	Composite scaffolds of CS and CMC reinforced with 50 wt% triphasic fibers were superior in terms of mechanical and biological properties and showed compressive strength and modulus of 150 kPa and 3.08 MPa, respectively, which is up to 300% greater than pure CS scaffolds.
30948070	2	92	from	effect	403:408	arg1	properties					503:512	the mechanical, physicochemical and biological properties	456:512	the mechanical, physicochemical and biological properties of the composite scaffolds	456:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	2	93	theme	mechanical	460:469	arg1	properties					503:512	the mechanical, physicochemical and biological properties	456:512	the mechanical, physicochemical and biological properties of the composite scaffolds	456:539	The effect of addition of CMC, fiber type and content on the mechanical, physicochemical and biological properties of the composite scaffolds was evaluated.
30948070	4	94	theme	Biphasic	643:650	arg1	fibers					652:657	Biphasic fibers	643:657	Biphasic fibers	643:657	Biphasic fibers contained two phases of hydroxyapatite (HA) and monetite, and triphasic fibers consisted of HA, β-tricalcium phosphate and calcium pyrophosphate and were 20-270 μm and 20-145 μm in length, respectively.
30948070	5	95	with	microstructures	906:920	arg1	porosity					932:939	high porosity	927:939	high porosity (61-75%)	927:948	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	5	95	with	microstructures	906:920	arg1	%					947:947	61-75%	942:947	61-75%	942:947	The composite scaffolds exhibited desirable microstructures with high porosity (61-75%) and interconnected pores in range of 35-200 μm.
30948070	1	96	theme	cellulose	265:273	arg1	scaffolds					220:228	porous scaffolds	213:228	porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC)	213:279	In this study porous scaffolds of chitosan (CS) and carboxymethyl cellulose (CMC) reinforced with whisker-like biphasic and triphasic calcium phosphate fibers were fabricated by freeze drying method.
30036810	9	0	theme	1.04	1763:1766	arg1	days					1735:1738	3 days	1733:1738	3 days	1733:1738	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	9	0	theme	1.04	1763:1766	arg1	CH4/d					1771:1775	1.04 NL CH4/d	1763:1775	1.04 NL CH4/d respectively	1763:1788	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	8	1	theme	cycle	1573:1577	arg1	days					1597:1600	5, 3 and 2 days	1586:1600	5, 3 and 2 days	1586:1600	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	8	1	theme	cycle	1573:1577	arg1	times					1579:1583	three different cycle times	1557:1583	three different cycle times (5, 3 and 2 days)	1557:1601	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	3	2	theme	different	667:675	arg1	levels					677:682	different levels	667:682	different levels of temperature, acid concentration and hydrolysis time	667:737	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	0	3	theme	process	165:171	arg1	optimization					115:126	batch optimization	109:126	batch optimization	109:126	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	0	3	theme	process	165:171	arg1	catalyst					92:99	Acid catalyst effect, batch optimization and stability of the semi-continuous process	87:171	catalyst	92:99	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	0	3	theme	process	165:171	arg1	stability					132:140	stability	132:140	stability of the semi-continuous process	132:171	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	1	4	theme	manufacturing	237:249	arg1	waste					216:220	the main solid waste	201:220	the main solid waste of the tequila manufacturing	201:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	1	4	theme	manufacturing	237:249	arg1	bagasse					190:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	3	5	theme	concentration	705:717	arg1	levels					677:682	different levels	667:682	different levels of temperature, acid concentration and hydrolysis time	667:737	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	10	6	theme	cycle	1812:1816	arg1	times					1818:1822	shorter cycle times	1804:1822	shorter cycle times	1804:1822	Operation at shorter cycle times is not advised due to microbial imbalance.
30036810	4	7	theme	H2SO4	845:849	arg1	bagasse					887:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	4	7	theme	H2SO4	845:849	arg1	one					851:853	the H2SO4 one	841:853	the H2SO4 one	841:853	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	6	8	theme	four-fold	1150:1158	arg1	increase					1160:1167	a four-fold increase	1148:1167	a four-fold increase	1148:1167	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	1	9	theme	tequilana	180:188	arg1	waste					216:220	the main solid waste	201:220	the main solid waste of the tequila manufacturing	201:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	1	9	theme	tequilana	180:188	arg1	bagasse					190:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	5	10	contain	contained	931:939	arg1	hydrolysate					919:929	the H2SO4 hydrolysate	909:929	the H2SO4 hydrolysate	909:929	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	5	10	contain	contained	931:939	arg2	concentrations					948:961	higher concentrations	941:961	higher concentrations of potential inhibitory compounds (furans and acetic acid)	941:1020	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	3	11	theme	hydrolysis	723:732	arg1	time					734:737	hydrolysis time	723:737	hydrolysis time	723:737	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	9	12	theme	stable	1619:1624	arg1	performances					1634:1645	stable reactor performances	1619:1645	stable reactor performances	1619:1645	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	7	13	theme	sugar	1386:1390	arg1	recovery					1392:1399	sugar recovery	1386:1399	sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min)	1386:1461	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	5	14	dep	compounds	987:995	arg1	furans					998:1003	furans	998:1003	furans	998:1003	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	5	14	dep	compounds	987:995	arg1	acid					1016:1019	acetic acid	1009:1019	acetic acid	1009:1019	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	1	15	theme	main	205:208	arg1	waste					216:220	the main solid waste	201:220	the main solid waste of the tequila manufacturing	201:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	1	15	theme	main	205:208	arg1	bagasse					190:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	4	16	theme	total	872:876	arg1	bagasse					887:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	4	16	theme	total	872:876	arg1	one					851:853	the H2SO4 one	841:853	the H2SO4 one	841:853	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	2	17	theme	batch	555:559	arg1	AnSBR					592:596	AnSBR	592:596	AnSBR	592:596	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	17	theme	batch	555:559	arg1	reactors					582:589	batch and sequencing batch reactors	555:589	batch and sequencing batch reactors (AnSBR)	555:597	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	8	18	from	times	1579:1583	arg1	stability					1544:1552	its stability	1540:1552	its stability at three different cycle times (5, 3 and 2 days)	1540:1601	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	9	19	theme	cycle	1650:1654	arg1	times					1656:1660	cycle times	1650:1660	cycle times of 5 and 3 days	1650:1676	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	2	20	with	pretreatments	453:465	arg1	H2SO4					480:484	H2SO4	480:484	H2SO4	480:484	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	20	with	pretreatments	453:465	arg1	HCl					472:474	HCl	472:474	HCl	472:474	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	21	theme	sugar	490:494	arg1	recovery					496:503	sugar recovery	490:503	sugar recovery	490:503	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	7	22	theme	acid	1218:1221	arg1	catalyst					1223:1230	acid catalyst	1218:1230	acid catalyst	1218:1230	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	8	23	used	used	1492:1495	arg2	conditions					1476:1485	This softer conditions	1464:1485	This softer conditions	1464:1485	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	0	24	theme	batch	71:75	arg1	reactors					77:84	batch and sequencing batch reactors	50:84	batch and sequencing batch reactors	50:84	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	1	25	theme	biofuel	326:332	arg1	production					334:343	biofuel production	326:343	biofuel production due to its lignocellulosic composition	326:382	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	9	26	theme	days	1673:1676	arg1	times					1656:1660	cycle times	1650:1660	cycle times of 5 and 3 days	1650:1676	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	0	27	dep	catalyst	92:99	arg1	effect					101:106	effect	101:106	effect	101:106	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	6	28	theme	batch	1044:1048	arg1	assays					1050:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	4	29	theme	optimal	763:769	arg1	conditions					771:780	optimal conditions	763:780	optimal conditions	763:780	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	7	30	theme	second	1182:1187	arg1	optimization					1189:1200	A second optimization	1180:1200	A second optimization by using HCl as acid catalyst and methane production as the response variable	1180:1278	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	5	31	theme	inhibitory	976:985	arg1	compounds					987:995	potential inhibitory compounds	966:995	potential inhibitory compounds (furans and acetic acid)	966:1020	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	9	32	theme	highest	1693:1699	arg1	yield					1709:1713	the highest methane yield	1689:1713	the highest methane yield	1689:1713	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	0	33	theme	Agave	0:4	arg1	bagasse					16:22	Agave tequilana bagasse	0:22	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.	0:172	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	5	34	theme	higher	941:946	arg1	concentrations					948:961	higher concentrations	941:961	higher concentrations of potential inhibitory compounds (furans and acetic acid)	941:1020	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	7	35	theme	hydrolysis	1305:1314	arg1	conditions					1316:1325	softer hydrolysis conditions	1298:1325	softer hydrolysis conditions	1298:1325	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	0	36	theme	methane	28:34	arg1	production					36:45	methane production	28:45	methane production in batch and sequencing batch reactors	28:84	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	6	37	theme	Subsequent	1023:1032	arg1	assays					1050:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	4	38	theme	sugar	819:823	arg1	recoveries					825:834	higher sugar recoveries	812:834	higher sugar recoveries	812:834	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	7	39	dep	%	1437:1437	arg1	130 °C					1444:1449	130 °C	1444:1449	130 °C	1444:1449	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	39	dep	%	1437:1437	arg1	HCl					1439:1441	HCl	1439:1441	HCl	1439:1441	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	39	dep	%	1437:1437	arg1	133min					1455:1460	133min	1455:1460	133min	1455:1460	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	1	40	theme	solid	210:214	arg1	waste					216:220	the main solid waste	201:220	the main solid waste of the tequila manufacturing	201:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	1	40	theme	solid	210:214	arg1	bagasse					190:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	0	41	dep	bagasse	16:22	arg1	optimization					115:126	batch optimization	109:126	batch optimization	109:126	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	0	41	dep	bagasse	16:22	arg1	catalyst					92:99	Acid catalyst effect, batch optimization and stability of the semi-continuous process	87:171	catalyst	92:99	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	0	41	dep	bagasse	16:22	arg1	stability					132:140	stability	132:140	stability of the semi-continuous process	132:171	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	6	42	theme	suitable	1105:1112	arg1	substrate					1114:1122	a more suitable substrate	1098:1122	a more suitable substrate for methane production	1098:1145	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	6	42	theme	suitable	1105:1112	arg1	hydrolysate					1083:1093	the HCl hydrolysate	1075:1093	the HCl hydrolysate	1075:1093	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	9	43	theme	NL	1746:1747	arg1	CH4/g-COD					1749:1757	0.28 NL CH4/g-COD	1741:1757	0.28 NL CH4/g-COD	1741:1757	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	9	43	theme	NL	1746:1747	arg1	days					1735:1738	3 days	1733:1738	3 days	1733:1738	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	3	44	theme	composite	646:654	arg1	designs					656:662	central composite designs	638:662	central composite designs	638:662	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	4	45	theme	HCl	787:789	arg1	pretreatment					791:802	the HCl pretreatment	783:802	the HCl pretreatment	783:802	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	3	46	theme	temperature	687:697	arg1	levels					677:682	different levels	667:682	different levels of temperature, acid concentration and hydrolysis time	667:737	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	10	47	theme	shorter	1804:1810	arg1	times					1818:1822	shorter cycle times	1804:1822	shorter cycle times	1804:1822	Operation at shorter cycle times is not advised due to microbial imbalance.
30036810	1	48	theme	tequila	229:235	arg1	manufacturing					237:249	the tequila manufacturing	225:249	the tequila manufacturing	225:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	9	49	theme	NL	1768:1769	arg1	days					1735:1738	3 days	1733:1738	3 days	1733:1738	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	9	49	theme	NL	1768:1769	arg1	CH4/d					1771:1775	1.04 NL CH4/d	1763:1775	1.04 NL CH4/d respectively	1763:1788	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	8	50	theme	different	1563:1571	arg1	days					1597:1600	5, 3 and 2 days	1586:1600	5, 3 and 2 days	1586:1600	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	8	50	theme	different	1563:1571	arg1	times					1579:1583	three different cycle times	1557:1583	three different cycle times (5, 3 and 2 days)	1557:1601	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	2	51	theme	batch	576:580	arg1	AnSBR					592:596	AnSBR	592:596	AnSBR	592:596	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	51	theme	batch	576:580	arg1	reactors					582:589	batch and sequencing batch reactors	555:589	batch and sequencing batch reactors (AnSBR)	555:597	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	6	52	theme	methane	1128:1134	arg1	production					1136:1145	methane production	1128:1145	methane production	1128:1145	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	3	53	theme	acid	700:703	arg1	concentration					705:717	acid concentration	700:717	acid concentration	700:717	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	1	54	theme	Agave	174:178	arg1	waste					216:220	the main solid waste	201:220	the main solid waste of the tequila manufacturing	201:249	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	1	54	theme	Agave	174:178	arg1	bagasse					190:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse	174:196	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	0	55	theme	batch	109:113	arg1	optimization					115:126	batch optimization	109:126	batch optimization	109:126	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	3	56	theme	time	734:737	arg1	levels					677:682	different levels	667:682	different levels of temperature, acid concentration and hydrolysis time	667:737	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	7	57	dep	recovery	1392:1399	arg1	%					1405:1405	1.8%	1402:1405	1.8% HCl, 119 °C and 103min	1402:1428	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	57	dep	recovery	1392:1399	arg1	%					1437:1437	1.9%	1434:1437	1.9% HCl, 130 °C and 133min	1434:1460	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	9	58	theme	reactor	1626:1632	arg1	performances					1634:1645	stable reactor performances	1619:1645	stable reactor performances	1619:1645	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	10	59	from	times	1818:1822	arg1	Operation					1791:1799	Operation	1791:1799	Operation at shorter cycle times	1791:1822	Operation at shorter cycle times is not advised due to microbial imbalance.
30036810	10	60	theme	microbial	1846:1854	arg1	imbalance					1856:1864	microbial imbalance	1846:1864	microbial imbalance	1846:1864	Operation at shorter cycle times is not advised due to microbial imbalance.
30036810	0	61	theme	semi-continuous	149:163	arg1	process					165:171	the semi-continuous process	145:171	the semi-continuous process	145:171	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	3	62	theme	Sugar	600:604	arg1	recovery					606:613	Sugar recovery	600:613	Sugar recovery	600:613	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	4	63	theme	sugars/g	878:885	arg1	bagasse					887:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	0.39 vs. 0.26 g total sugars/g bagasse	856:893	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	4	63	theme	sugars/g	878:885	arg1	one					851:853	the H2SO4 one	841:853	the H2SO4 one	841:853	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	2	64	theme	sequencing	565:574	arg1	AnSBR					592:596	AnSBR	592:596	AnSBR	592:596	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	64	theme	sequencing	565:574	arg1	reactors					582:589	batch and sequencing batch reactors	555:589	batch and sequencing batch reactors (AnSBR)	555:597	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	0	65	theme	sequencing	60:69	arg1	reactors					77:84	batch and sequencing batch reactors	50:84	batch and sequencing batch reactors	50:84	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	7	66	theme	methane	1352:1358	arg1	production					1360:1369	methane production	1352:1369	methane production	1352:1369	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	67	dep	%	1405:1405	arg1	119 °C					1412:1417	119 °C	1412:1417	119 °C	1412:1417	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	67	dep	%	1405:1405	arg1	HCl					1407:1409	HCl	1407:1409	HCl	1407:1409	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	67	dep	%	1405:1405	arg1	103min					1423:1428	103min	1423:1428	103min	1423:1428	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	8	68	theme	softer	1469:1474	arg1	conditions					1476:1485	This softer conditions	1464:1485	This softer conditions	1464:1485	This softer conditions were used to feed an AnSBR for 110 days and evaluate its stability at three different cycle times (5, 3 and 2 days).
30036810	0	69	theme	Acid	87:90	arg1	catalyst					92:99	Acid catalyst effect, batch optimization and stability of the semi-continuous process	87:171	catalyst	92:99	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	5	70	theme	potential	966:974	arg1	compounds					987:995	potential inhibitory compounds	966:995	potential inhibitory compounds (furans and acetic acid)	966:1020	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	6	71	theme	HCl	1079:1081	arg1	substrate					1114:1122	a more suitable substrate	1098:1122	a more suitable substrate for methane production	1098:1145	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	6	71	theme	HCl	1079:1081	arg1	hydrolysate					1083:1093	the HCl hydrolysate	1075:1093	the HCl hydrolysate	1075:1093	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	0	72	from	production	36:45	arg1	reactors					77:84	batch and sequencing batch reactors	50:84	batch and sequencing batch reactors	50:84	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	0	73	theme	tequilana	6:14	arg1	bagasse					16:22	Agave tequilana bagasse	0:22	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.	0:172	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	1	74	theme	lignocellulosic	356:370	arg1	composition					372:382	its lignocellulosic composition	352:382	its lignocellulosic composition	352:382	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	5	75	theme	H2SO4	913:917	arg1	hydrolysate					919:929	the H2SO4 hydrolysate	909:929	the H2SO4 hydrolysate	909:929	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	9	76	theme	methane	1701:1707	arg1	yield					1709:1713	the highest methane yield	1689:1713	the highest methane yield	1689:1713	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	1	77	theme	environmental	269:281	arg1	issue					283:287	an environmental issue	266:287	an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance	266:396	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	7	78	theme	softer	1298:1303	arg1	conditions					1316:1325	softer hydrolysis conditions	1298:1325	softer hydrolysis conditions	1298:1325	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	5	79	theme	acetic	1009:1014	arg1	acid					1016:1019	acetic acid	1009:1019	acetic acid	1009:1019	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	6	80	theme	anaerobic	1034:1042	arg1	assays					1050:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays	1023:1055	Subsequent anaerobic batch assays demonstrated that the HCl hydrolysate is a more suitable substrate for methane production; a four-fold increase was found.
30036810	0	81	theme	batch	50:54	arg1	reactors					77:84	batch and sequencing batch reactors	50:84	batch and sequencing batch reactors	50:84	Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process.
30036810	7	82	theme	response	1262:1269	arg1	HCl					1211:1213	HCl	1211:1213	HCl	1211:1213	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	7	82	theme	response	1262:1269	arg1	variable					1271:1278	the response variable	1258:1278	the response variable	1258:1278	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	4	83	theme	higher	812:817	arg1	recoveries					825:834	higher sugar recoveries	812:834	higher sugar recoveries	812:834	Results showed that at optimal conditions, the HCl pretreatment induced higher sugar recoveries than the H2SO4 one, 0.39 vs. 0.26 g total sugars/g bagasse.
30036810	1	84	theme	potential	302:310	arg1	feedstock					312:320	a potential feedstock	300:320	an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance	266:396	Agave tequilana bagasse is the main solid waste of the tequila manufacturing and represents an environmental issue as well as a potential feedstock for biofuel production due to its lignocellulosic composition and abundance.
30036810	5	85	theme	compounds	987:995	arg1	concentrations					948:961	higher concentrations	941:961	higher concentrations of potential inhibitory compounds (furans and acetic acid)	941:1020	Furthermore, the H2SO4 hydrolysate contained higher concentrations of potential inhibitory compounds (furans and acetic acid).
30036810	9	86	theme	0.28	1741:1744	arg1	CH4/g-COD					1749:1757	0.28 NL CH4/g-COD	1741:1757	0.28 NL CH4/g-COD	1741:1757	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	9	86	theme	0.28	1741:1744	arg1	days					1735:1738	3 days	1733:1738	3 days	1733:1738	Results showed stable reactor performances at cycle times of 5 and 3 days, obtaining the highest methane yield and production at 3 days, 0.28 NL CH4/g-COD and 1.04 NL CH4/d respectively.
30036810	3	87	theme	central	638:644	arg1	designs					656:662	central composite designs	638:662	central composite designs	638:662	Sugar recovery was optimized by using central composite designs at different levels of temperature, acid concentration and hydrolysis time.
30036810	7	88	theme	methane	1236:1242	arg1	production					1244:1253	methane production	1236:1253	methane production	1236:1253	A second optimization by using HCl as acid catalyst and methane production as the response variable demonstrated that softer hydrolysis conditions are required to optimize methane production as compared to sugar recovery (1.8% HCl, 119 °C and 103min vs. 1.9% HCl, 130 °C and 133min).
30036810	2	89	from	hydrolysates	539:550	arg1	AnSBR					592:596	AnSBR	592:596	AnSBR	592:596	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30036810	2	89	from	hydrolysates	539:550	arg1	reactors					582:589	batch and sequencing batch reactors	555:589	batch and sequencing batch reactors (AnSBR)	555:597	In this contribution, this feedstock was subjected to pretreatments with HCl and H2SO4 for sugar recovery and methane was produced from the hydrolysates in batch and sequencing batch reactors (AnSBR).
30070023	5	0	theme	in	1084:1085	arg1	trials					1105:1110	in vitro and in vivo trials	1084:1110	in vitro and in vivo trials for the glass-ceramic-polymer composites	1084:1151	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	1	theme	macroscopic	843:853	arg1	alterations					855:865	clear macroscopic alterations	837:865	clear macroscopic alterations	837:865	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	1	2	theme	bioactive	155:163	arg1	glass-ceramics					181:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics	155:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide	155:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	2	3	theme	high-resolution	532:546	arg1	microscopy					570:579	high-resolution transmission electron microscopy	532:579	high-resolution transmission electron microscopy	532:579	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	0	4	theme	regeneration	83:94	arg1	trials					96:101	bone tissue regeneration trials	71:101	bone tissue regeneration trials	71:101	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	5	5	theme	crystalline	1029:1039	arg1	shapes					1041:1046	Different crystalline shapes	1019:1046	Different crystalline shapes of hydroxyapatite	1019:1064	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	6	theme	clear	837:841	arg1	alterations					855:865	clear macroscopic alterations	837:865	clear macroscopic alterations	837:865	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	1	7	theme	SiO2	165:168	arg1	glass-ceramics					181:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics	155:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide	155:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	4	8	dep	in	968:969	arg1	vivo					971:974	vivo	971:974	vivo	971:974	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	3	9	dep	in	740:741	arg1	vivo					743:746	vivo	743:746	vivo	743:746	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	2	10	theme	electron	561:568	arg1	microscopy					570:579	high-resolution transmission electron microscopy	532:579	high-resolution transmission electron microscopy	532:579	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	5	11	theme	glass-ceramic-polymer	1120:1140	arg1	composites					1142:1151	the glass-ceramic-polymer composites	1116:1151	the glass-ceramic-polymer composites	1116:1151	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	7	12	theme	suitable	1516:1523	arg1	candidates					1525:1534	any doubt suitable candidates	1506:1534	any doubt suitable candidates for future bone regeneration applications	1506:1576	These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.
30070023	6	13	theme	good	1317:1320	arg1	biocompatibility					1322:1337	a good biocompatibility	1315:1337	a good biocompatibility with no significant signs of rejection by the host tissue	1315:1395	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	2	14	theme	transmission	548:559	arg1	microscopy					570:579	high-resolution transmission electron microscopy	532:579	high-resolution transmission electron microscopy	532:579	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	5	15	theme	precipitated	1167:1178	arg1	nodular					1204:1210	nodular	1204:1210	nodular	1204:1210	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	5	15	theme	precipitated	1167:1178	arg1	apatite					1180:1186	the in vitro precipitated apatite	1154:1186	the in vitro precipitated apatite	1154:1186	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	5	16	theme	hydroxyapatite	1051:1064	arg1	shapes					1041:1046	Different crystalline shapes	1019:1046	Different crystalline shapes of hydroxyapatite	1019:1064	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	17	theme	globular	991:998	arg1	shape					1000:1004	a globular shape	989:1004	a globular shape	989:1004	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	4	18	theme	recovered	763:771	arg1	composites					773:782	The recovered composites	759:782	The recovered composites after 5 weeks' in vivo and in vitro trials	759:825	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	1	19	theme	-CaO-P2	170:176	arg1	glass-ceramics					181:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics	155:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide	155:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	5	20	dep	in	1158:1159	arg1	vitro					1161:1165	vitro	1161:1165	vitro	1161:1165	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	5	21	dep	in	1097:1098	arg1	vivo					1100:1103	vivo	1100:1103	vivo	1100:1103	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	1	22	with	pullulan	141:148	arg1	oxide					208:212	copper oxide	201:212	copper oxide	201:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	4	23	dep	in	811:812	arg1	vitro					814:818	vitro	814:818	vitro	814:818	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	1	24	theme	O5	178:179	arg1	glass-ceramics					181:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics	155:194	bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide	155:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	0	25	theme	alginate-pullulan-bioactive	4:30	arg1	composites					38:47	New alginate-pullulan-bioactive glass composites	0:47	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.	0:102	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	4	26	theme	flake	938:942	arg1	aspect					944:949	a corn flake aspect	931:949	a corn flake aspect	931:949	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	2	27	theme	scanning	375:382	arg1	SEM					405:407	SEM	405:407	SEM	405:407	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	2	27	theme	scanning	375:382	arg1	microscopy					393:402	scanning electron microscopy	375:402	scanning electron microscopy (SEM)	375:408	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	0	28	theme	New	0:2	arg1	composites					38:47	New alginate-pullulan-bioactive glass composites	0:47	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.	0:102	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	3	29	theme	good	735:738	arg1	tolerance					748:756	a good in vivo tolerance	733:756	a good in vivo tolerance	733:756	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	1	30	with	alginate	131:138	arg1	oxide					208:212	copper oxide	201:212	copper oxide	201:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	2	31	theme	FT-IR	325:329	arg1	spectroscopy					332:343	infrared (FT-IR) spectroscopy	315:343	infrared (FT-IR) spectroscopy	315:343	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	4	32	contain	have	926:929	arg2	aspect					944:949	a corn flake aspect	931:949	a corn flake aspect	931:949	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	4	32	contain	have	926:929	arg1	they					921:924	they	921:924	they	921:924	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	3	33	theme	in	740:741	arg1	tolerance					748:756	a good in vivo tolerance	733:756	a good in vivo tolerance	733:756	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	4	34	theme	simulated	899:907	arg1	fluid					914:918	simulated body fluid	899:918	simulated body fluid	899:918	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	5	35	dep	in	1221:1222	arg1	vivo					1224:1227	vivo	1224:1227	vivo	1224:1227	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	1	36	theme	copper	201:206	arg1	oxide					208:212	copper oxide	201:212	copper oxide	201:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	0	37	theme	glass	32:36	arg1	composites					38:47	New alginate-pullulan-bioactive glass composites	0:47	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.	0:102	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	7	38	theme	future	1540:1545	arg1	applications					1565:1576	future bone regeneration applications	1540:1576	future bone regeneration applications	1540:1576	These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.
30070023	6	39	theme	Histopathological	1282:1298	arg1	results					1300:1306	Histopathological results	1282:1306	Histopathological results	1282:1306	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	2	40	theme	infrared	315:322	arg1	spectroscopy					332:343	infrared (FT-IR) spectroscopy	315:343	infrared (FT-IR) spectroscopy	315:343	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	5	41	theme	needlelike	1247:1256	arg1	formation					1271:1279	needlelike crystallites formation	1247:1279	needlelike crystallites formation	1247:1279	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	0	42	theme	copper	54:59	arg1	oxide					61:65	copper oxide	54:65	copper oxide	54:65	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	5	43	dep	in	1084:1085	arg1	vitro					1087:1091	vitro	1087:1091	vitro	1087:1091	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	5	44	theme	Different	1019:1027	arg1	shapes					1041:1046	Different crystalline shapes	1019:1046	Different crystalline shapes of hydroxyapatite	1019:1064	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	45	theme	in	799:800	arg1	trials					820:825	in vivo and in vitro trials	799:825	5 weeks' in vivo and in vitro trials	790:825	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	5	46	theme	crystallites	1258:1269	arg1	formation					1271:1279	needlelike crystallites formation	1247:1279	needlelike crystallites formation	1247:1279	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	47	dep	in	799:800	arg1	vivo					802:805	vivo	802:805	vivo	802:805	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	4	48	dep	5 weeks	790:796	arg1	trials					820:825	in vivo and in vitro trials	799:825	5 weeks' in vivo and in vitro trials	790:825	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	5	49	theme	in	1097:1098	arg1	trials					1105:1110	in vitro and in vivo trials	1084:1110	in vitro and in vivo trials for the glass-ceramic-polymer composites	1084:1151	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	4	50	theme	body	909:912	arg1	fluid					914:918	simulated body fluid	899:918	simulated body fluid	899:918	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	3	51	theme	cell	612:615	arg1	assays					627:632	The fibroblast and osteoblast cell viability assays	582:632	assays	627:632	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	0	52	with	composites	38:47	arg1	oxide					61:65	copper oxide	54:65	copper oxide	54:65	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	4	53	theme	in	968:969	arg1	inoculation					976:986	their in vivo inoculation	962:986	their in vivo inoculation	962:986	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	6	54	theme	rejection	1368:1376	arg1	signs					1359:1363	no significant signs	1344:1363	no significant signs of rejection by the host tissue	1344:1395	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	1	55	with	glass-ceramics	181:194	arg1	oxide					208:212	copper oxide	201:212	copper oxide	201:212	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	3	56	theme	viability	617:625	arg1	assays					627:632	The fibroblast and osteoblast cell viability assays	582:632	assays	627:632	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	1	57	theme	sodium	124:129	arg1	alginate					131:138	sodium alginate	124:138	sodium alginate	124:138	Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules.
30070023	2	58	theme	biocompatibility	437:452	arg1	properties					454:463	biocompatibility properties	437:463	biocompatibility properties	437:463	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	4	59	theme	in	811:812	arg1	trials					820:825	in vivo and in vitro trials	799:825	5 weeks' in vivo and in vitro trials	790:825	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	3	60	theme	proliferation	650:662	arg1	rates					664:668	good proliferation rates	645:668	good proliferation rates for all investigated samples	645:697	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	4	61	theme	corn	933:936	arg1	aspect					944:949	a corn flake aspect	931:949	a corn flake aspect	931:949	The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained.
30070023	2	62	theme	X-ray	346:350	arg1	XRD					365:367	XRD	365:367	XRD	365:367	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	2	62	theme	X-ray	346:350	arg1	diffraction					352:362	X-ray diffraction	346:362	X-ray diffraction (XRD)	346:368	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	5	63	theme	in	1221:1222	arg1	experiment					1229:1238	the in vivo experiment	1217:1238	the in vivo experiment	1217:1238	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	7	64	theme	studied	1462:1468	arg1	materials					1470:1478	the studied materials	1458:1478	the studied materials	1458:1478	These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.
30070023	3	65	theme	osteoblast	601:610	arg1	assays					627:632	The fibroblast and osteoblast cell viability assays	582:632	assays	627:632	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	6	66	theme	host	1385:1388	arg1	tissue					1390:1395	the host tissue	1381:1395	the host tissue	1381:1395	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	0	67	theme	tissue	76:81	arg1	trials					96:101	bone tissue regeneration trials	71:101	bone tissue regeneration trials	71:101	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	3	68	theme	good	645:648	arg1	rates					664:668	good proliferation rates	645:668	good proliferation rates for all investigated samples	645:697	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	6	69	theme	significant	1347:1357	arg1	signs					1359:1363	no significant signs	1344:1363	no significant signs of rejection by the host tissue	1344:1395	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	7	70	theme	regeneration	1552:1563	arg1	applications					1565:1576	future bone regeneration applications	1540:1576	future bone regeneration applications	1540:1576	These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.
30070023	2	71	theme	electron	384:391	arg1	SEM					405:407	SEM	405:407	SEM	405:407	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	2	71	theme	electron	384:391	arg1	microscopy					393:402	scanning electron microscopy	375:402	scanning electron microscopy (SEM)	375:408	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	7	72	theme	bone	1547:1550	arg1	applications					1565:1576	future bone regeneration applications	1540:1576	future bone regeneration applications	1540:1576	These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.
30070023	0	73	theme	bone	71:74	arg1	trials					96:101	bone tissue regeneration trials	71:101	bone tissue regeneration trials	71:101	New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials.
30070023	3	74	theme	investigated	678:689	arg1	samples					691:697	all investigated samples	674:697	all investigated samples	674:697	The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance.
30070023	2	75	theme	obtained	245:252	arg1	samples					254:260	The obtained samples	241:260	The obtained samples	241:260	The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy.
30070023	6	76	with	biocompatibility	1322:1337	arg1	signs					1359:1363	no significant signs	1344:1363	no significant signs of rejection by the host tissue	1344:1395	Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue.
30070023	5	77	theme	in	1158:1159	arg1	nodular					1204:1210	nodular	1204:1210	nodular	1204:1210	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
30070023	5	77	theme	in	1158:1159	arg1	apatite					1180:1186	the in vitro precipitated apatite	1154:1186	the in vitro precipitated apatite	1154:1186	Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation.
31718349	4	0	theme	metal-organic	790:802	arg1	matrix					804:809	metal-organic matrix	790:809	metal-organic matrix	790:809	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	6	1	theme	capacity	1006:1013	arg1	nanoparticles					1026:1038	adsorption capacity and silver nanoparticles	995:1038	adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance	995:1141	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	2	2	theme	silver	399:404	arg1	nitrate					406:412	silver nitrate	399:412	silver nitrate	399:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	1	3	theme	simple	288:293	arg1	method					322:327	simple sonication assisted reflux method	288:327	simple sonication assisted reflux method	288:327	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	0	4	theme	gum	87:89	arg1	synthesis					20:28	Sonication-assisted synthesis	0:28	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum	0:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	5	5	theme	silver	891:896	arg1	nanoparticles					898:910	metallic silver nanoparticles	882:910	metallic silver nanoparticles	882:910	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	3	6	theme	infra-red	588:596	arg1	spectroscopy					598:609	infra-red spectroscopy	588:609	infra-red spectroscopy	588:609	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	1	7	theme	sonication	295:304	arg1	method					322:327	simple sonication assisted reflux method	288:327	simple sonication assisted reflux method	288:327	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	1	8	theme	assisted	306:313	arg1	method					322:327	simple sonication assisted reflux method	288:327	simple sonication assisted reflux method	288:327	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	2	9	theme	gum	371:373	arg1	composition					342:352	Appropriate composition	330:352	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate	330:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	3	10	theme	scanning	634:641	arg1	microscopy					652:661	scanning electron microscopy	634:661	scanning electron microscopy	634:661	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	7	11	theme	reaction	1309:1316	arg1	time					1318:1321	short reaction time	1303:1321	short reaction time	1303:1321	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	2	12	theme	Appropriate	330:340	arg1	composition					342:352	Appropriate composition	330:352	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate	330:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	1	13	theme	reflux	315:320	arg1	method					322:327	simple sonication assisted reflux method	288:327	simple sonication assisted reflux method	288:327	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	0	14	theme	visible	123:129	arg1	photocatalyst					137:149	a visible light photocatalyst	121:149	a visible light photocatalyst	121:149	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	6	15	theme	silver	1019:1024	arg1	nanoparticles					1026:1038	adsorption capacity and silver nanoparticles	995:1038	adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance	995:1141	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	7	16	theme	90	1327:1328	arg1	%					1329:1329	%	1329:1329	%	1329:1329	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	3	17	theme	diffuse	531:537	arg1	spectrophotometer					551:567	UV-vis diffuse reflectance spectrophotometer	524:567	UV-vis diffuse reflectance spectrophotometer	524:567	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	3	18	theme	X-ray	612:616	arg1	diffractometer					618:631	X-ray diffractometer	612:631	X-ray diffractometer	612:631	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	7	19	theme	aniline	1254:1260	arg1	formation					1241:1249	the formation	1237:1249	the formation of aniline from nitrobenzene	1237:1278	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	5	20	theme	organic	850:856	arg1	structure					868:876	the organic framework structure	846:876	the organic framework structure	846:876	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	3	21	theme	synthesised	447:457	arg1	nanocomposites					459:472	The synthesised nanocomposites	443:472	The synthesised nanocomposites	443:472	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	3	22	dep	Fourier	570:576	arg1	transform					578:586	transform	578:586	transform infra-red spectroscopy	578:609	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	3	23	theme	photoluminescence	496:512	arg1	spectrum					514:521	photoluminescence spectrum	496:521	photoluminescence spectrum	496:521	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	7	24	theme	%	1329:1329	arg1	conversion					1331:1340	90% conversion	1327:1340	90% conversion	1327:1340	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	5	25	theme	metallic	882:889	arg1	nanoparticles					898:910	metallic silver nanoparticles	882:910	metallic silver nanoparticles	882:910	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	5	26	theme	framework	858:866	arg1	structure					868:876	the organic framework structure	846:876	the organic framework structure	846:876	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	1	27	dep	base	178:181	arg1	nanocomposites					246:259	ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	183:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	7	28	from	reaction	1213:1220	arg1	nanocomposite					1148:1160	The nanocomposite	1144:1160	The nanocomposite	1144:1160	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	7	28	from	reaction	1213:1220	arg1	selective					1186:1194	selective	1186:1194	selective	1186:1194	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	0	29	theme	Sonication-assisted	0:18	arg1	synthesis					20:28	Sonication-assisted synthesis	0:28	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum	0:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	6	30	theme	plasma	1126:1131	arg1	resonance					1133:1141	surface plasma resonance	1118:1141	surface plasma resonance	1118:1141	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	2	31	theme	guar	366:369	arg1	gum					371:373	purified guar gum	357:373	purified guar gum	357:373	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	1	32	theme	-Silver-Guar	216:227	arg1	nanocomposites					246:259	ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	183:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	4	33	theme	crystalline	705:715	arg1	peaks					717:721	The crystalline peaks	701:721	The crystalline peaks of XRD and FTIR	701:737	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	6	34	theme	surface	1118:1124	arg1	resonance					1133:1141	surface plasma resonance	1118:1141	surface plasma resonance	1118:1141	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	7	35	theme	single	1283:1288	arg1	product					1290:1296	single product	1283:1296	single product	1283:1296	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	2	36	theme	purified	357:364	arg1	gum					371:373	purified guar gum	357:373	purified guar gum	357:373	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	1	37	theme	gum	229:231	arg1	nanocomposites					246:259	ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	183:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	3	38	theme	transmission	667:678	arg1	microscopy					689:698	transmission electron microscopy	667:698	transmission electron microscopy	667:698	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	0	39	theme	light	131:135	arg1	photocatalyst					137:149	a visible light photocatalyst	121:149	a visible light photocatalyst	121:149	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	6	40	theme	adsorption	995:1004	arg1	capacity					1006:1013	adsorption capacity	995:1013	adsorption capacity	995:1013	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	1	41	theme	encapsulated	233:244	arg1	nanocomposites					246:259	ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	183:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	0	42	theme	heterostructured	39:54	arg1	base					63:66	a new heterostructured schiff base	33:66	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	5	43	theme	Morphology	812:821	arg1	studies					823:829	Morphology studies	812:829	Morphology studies	812:829	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	0	44	theme	new	35:37	arg1	base					63:66	a new heterostructured schiff base	33:66	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	6	45	theme	light	1093:1097	arg1	absorption					1099:1108	the visible light absorption	1081:1108	the visible light absorption	1081:1108	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	5	46	theme	organic	932:938	arg1	framework					940:948	the organic framework	928:948	the organic framework	928:948	Morphology studies have confirmed the organic framework structure and metallic silver nanoparticles are embedded on the organic framework.
31718349	0	47	theme	base	63:66	arg1	gum					87:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	6	48	theme	visible	1085:1091	arg1	absorption					1099:1108	the visible light absorption	1081:1108	the visible light absorption	1081:1108	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	6	49	theme	nanocomposites	969:982	arg1	efficiency					955:964	The efficiency	951:964	The efficiency of nanocomposites	951:982	The efficiency of nanocomposites depends on adsorption capacity and silver nanoparticles that are encapsulated thereby increasing the visible light absorption through surface plasma resonance.
31718349	3	50	theme	electron	680:687	arg1	microscopy					689:698	transmission electron microscopy	667:698	transmission electron microscopy	667:698	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	2	51	theme	ligand	388:393	arg1	composition					342:352	Appropriate composition	330:352	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate	330:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	0	52	theme	schiff	56:61	arg1	base					63:66	a new heterostructured schiff base	33:66	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	7	53	theme	hydrogenation	1199:1211	arg1	reaction					1213:1220	hydrogenation reaction	1199:1220	hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion	1199:1340	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	2	54	theme	base	383:386	arg1	ligand					388:393	Schiff base ligand	376:393	Schiff base ligand	376:393	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	7	55	theme	short	1303:1307	arg1	time					1318:1321	short reaction time	1303:1321	short reaction time	1303:1321	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	4	56	theme	guar	775:778	arg1	gum					780:782	guar gum	775:782	guar gum	775:782	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	2	57	theme	Schiff	376:381	arg1	ligand					388:393	Schiff base ligand	376:393	Schiff base ligand	376:393	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	3	58	theme	electron	643:650	arg1	microscopy					652:661	scanning electron microscopy	634:661	scanning electron microscopy	634:661	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	2	59	used	used	419:422	arg2	composition					342:352	Appropriate composition	330:352	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate	330:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	2	60	theme	nitrate	406:412	arg1	composition					342:352	Appropriate composition	330:352	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate	330:412	Appropriate composition of purified guar gum, Schiff base ligand and silver nitrate were used for the synthesis.
31718349	3	61	theme	reflectance	539:549	arg1	spectrophotometer					551:567	UV-vis diffuse reflectance spectrophotometer	524:567	UV-vis diffuse reflectance spectrophotometer	524:567	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	0	62	theme	Silver-Guar	75:85	arg1	gum					87:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	4	63	dep	base	759:762	arg1	ligand					764:769	ligand	764:769	ligand	764:769	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	3	64	theme	UV-vis	524:529	arg1	spectrophotometer					551:567	UV-vis diffuse reflectance spectrophotometer	524:567	UV-vis diffuse reflectance spectrophotometer	524:567	The synthesised nanocomposites were characterised by photoluminescence spectrum, UV-vis diffuse reflectance spectrophotometer, Fourier transform infra-red spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy.
31718349	1	65	theme	heterostructured	154:169	arg1	base					178:181	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
31718349	4	66	theme	Schiff	752:757	arg1	base					759:762	Schiff base ligand and guar gum	752:782	base	759:762	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	4	67	theme	XRD	726:728	arg1	peaks					717:721	The crystalline peaks	701:721	The crystalline peaks of XRD and FTIR	701:737	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	0	68	theme	ligand	68:73	arg1	gum					87:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	a new heterostructured schiff base ligand Silver-Guar gum	33:89	Sonication-assisted synthesis of a new heterostructured schiff base ligand Silver-Guar gum encapsulated nanocomposite as a visible light photocatalyst.
31718349	7	69	from	selective	1186:1194	arg1	reaction					1213:1220	hydrogenation reaction	1199:1220	hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion	1199:1340	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	4	70	theme	FTIR	734:737	arg1	peaks					717:721	The crystalline peaks	701:721	The crystalline peaks of XRD and FTIR	701:737	The crystalline peaks of XRD and FTIR reveals that Schiff base ligand and guar gum forms metal-organic matrix.
31718349	7	71	from	nitrobenzene	1267:1278	arg1	formation					1241:1249	the formation	1237:1249	the formation of aniline from nitrobenzene	1237:1278	The nanocomposite was proved to be highly selective in hydrogenation reaction which favoured the formation of aniline from nitrobenzene as single product with short reaction time and 90% conversion.
31718349	1	72	theme	Schiff	171:176	arg1	base					178:181	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites	152:259	A heterostructured Schiff base ligand (Benzildiethylenetriamine)-Silver-Guar gum encapsulated nanocomposites was intended to prepare by simple sonication assisted reflux method.
29753183	2	0	from	values	401:406	arg1	form					386:389	solution form	377:389	solution form at all pH values	377:406	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	1	1	theme	pure	145:148	arg1	hydrogels					164:172	The pure and composite hydrogels	141:172	The pure and composite hydrogels of chitosan with sodium alginate	141:205	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	0	2	theme	alginate	74:81	arg1	hydrogels					48:56	pH responsive composite hydrogels	24:56	pH responsive composite hydrogels of chitosan and alginate	24:81	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	3	from	applicability	1347:1359	arg1	industry					1377:1384	industry	1377:1384	industry	1377:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	7	3	from	applicability	1347:1359	arg1	research					1364:1371	research	1364:1371	research	1364:1371	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	5	4	theme	synthetic	883:891	arg1	medium					893:898	the synthetic medium	879:898	the synthetic medium	879:898	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	3	5	with	hydrogels	483:491	arg1	dynamics					519:526	modulated structural dynamics	498:526	modulated structural dynamics	498:526	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	3	5	with	hydrogels	483:491	arg1	viscoelasticity					532:546	viscoelasticity	532:546	viscoelasticity	532:546	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	7	6	with	hydrogels	1165:1173	arg1	properties					1189:1198	modified properties	1180:1198	modified properties	1180:1198	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	7	theme	chitosan	741:748	arg1	percentage					727:736	more percentage	722:736	more percentage of chitosan	722:748	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	7	8	from	research	1364:1371	arg1	niche					1334:1338	niche	1334:1338	niche of its applicability in research and industry	1334:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	7	9	theme	simple	1219:1224	arg1	changes					1226:1232	simple changes	1219:1232	simple changes in experimental conditions exploiting physical interactions	1219:1292	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	7	10	theme	physical	1272:1279	arg1	interactions					1281:1292	physical interactions	1272:1292	physical interactions	1272:1292	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	2	11	theme	sodium	350:355	arg1	alginate					357:364	sodium alginate	350:364	sodium alginate	350:364	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	3	12	theme	pH	467:468	arg1	adjustment					420:429	careful adjustment	412:429	careful adjustment of mixing weight fraction ratio and pH	412:468	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	4	13	theme	higher	627:632	arg1	fraction					641:648	a higher weight fraction	625:648	a higher weight fraction of alginate	625:660	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	3	14	theme	ratio	457:461	arg1	adjustment					420:429	careful adjustment	412:429	careful adjustment of mixing weight fraction ratio and pH	412:468	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	3	15	theme	modulated	498:506	arg1	dynamics					519:526	modulated structural dynamics	498:526	modulated structural dynamics	498:526	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	4	16	theme	alginate	653:660	arg1	fraction					641:648	a higher weight fraction	625:648	a higher weight fraction of alginate	625:660	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	1	17	theme	composite	154:162	arg1	hydrogels					164:172	The pure and composite hydrogels	141:172	The pure and composite hydrogels of chitosan with sodium alginate	141:205	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	5	18	theme	composite	785:793	arg1	hydrogels					795:803	Strongest composite hydrogels	775:803	Strongest composite hydrogels	775:803	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	7	19	theme	composite	1155:1163	arg1	hydrogels					1165:1173	composite hydrogels	1155:1173	composite hydrogels with modified properties	1155:1198	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	3	20	theme	structural	508:517	arg1	dynamics					519:526	modulated structural dynamics	498:526	modulated structural dynamics	498:526	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	7	21	dep	pH.	1113:1115	arg1	highlights					1128:1137	highlights	1128:1137	highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry	1128:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	22	theme	weight	634:639	arg1	fraction					641:648	a higher weight fraction	625:648	a higher weight fraction of alginate	625:660	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	3	23	theme	mechanical	549:558	arg1	strength					560:567	mechanical strength	549:567	mechanical strength	549:567	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	2	24	theme	solution	377:384	arg1	form					386:389	solution form	377:389	solution form at all pH values	377:406	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	0	25	theme	Rheological	0:10	arg1	behavior					12:19	Rheological behavior	0:19	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.	0:139	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	26	theme	composite	1073:1081	arg1	hydrogels					1083:1091	the composite hydrogels	1069:1091	the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry	1069:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	27	theme	basic	713:717	arg1	pH					719:720	basic pH	713:720	basic pH	713:720	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	1	28	theme	chitosan	177:184	arg1	hydrogels					164:172	The pure and composite hydrogels	141:172	The pure and composite hydrogels of chitosan with sodium alginate	141:205	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	0	29	theme	pH	24:25	arg1	hydrogels					48:56	pH responsive composite hydrogels	24:56	pH responsive composite hydrogels of chitosan and alginate	24:81	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	30	from	industry	1377:1384	arg1	niche					1334:1338	niche	1334:1338	niche of its applicability in research and industry	1334:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	3	31	theme	fraction	448:455	arg1	ratio					457:461	mixing weight fraction ratio	434:461	mixing weight fraction ratio	434:461	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	7	32	theme	modified	1180:1187	arg1	properties					1189:1198	modified properties	1180:1198	modified properties	1180:1198	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	6	33	theme	encapsulation	950:962	arg1	capability					964:973	their encapsulation capability	944:973	their encapsulation capability towards Citral	944:988	The synthesized hydrogels were studied for their encapsulation capability towards Citral.
29753183	7	34	from	niche	1334:1338	arg1	industry					1377:1384	industry	1377:1384	industry	1377:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	7	34	from	niche	1334:1338	arg1	research					1364:1371	research	1364:1371	research	1364:1371	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	35	theme	more	722:725	arg1	percentage					727:736	more percentage	722:736	more percentage of chitosan	722:748	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	1	36	theme	sodium	191:196	arg1	alginate					198:205	sodium alginate	191:205	sodium alginate	191:205	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	0	37	theme	composite	38:46	arg1	hydrogels					48:56	pH responsive composite hydrogels	24:56	pH responsive composite hydrogels of chitosan and alginate	24:81	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	38	theme	better	1310:1315	arg1	understanding					1317:1329	better understanding	1310:1329	better understanding of niche of its applicability in research and industry	1310:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	0	39	theme	citral	133:138	arg1	encapsulation					116:128	encapsulation	116:128	encapsulation of citral	116:138	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	3	40	theme	mixing	434:439	arg1	ratio					457:461	mixing weight fraction ratio	434:461	mixing weight fraction ratio	434:461	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	3	41	theme	careful	412:418	arg1	adjustment					420:429	careful adjustment	412:429	careful adjustment of mixing weight fraction ratio and pH	412:468	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	2	42	theme	pH	398:399	arg1	values					401:406	all pH values	394:406	all pH values	394:406	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	0	43	theme	responsive	27:36	arg1	hydrogels					48:56	pH responsive composite hydrogels	24:56	pH responsive composite hydrogels of chitosan and alginate	24:81	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	44	theme	hydrogels	1165:1173	arg1	range					1146:1150	a range	1144:1150	a range of composite hydrogels with modified properties	1144:1198	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	45	theme	acidic	614:619	arg1	pH					621:622	acidic pH	614:622	acidic pH	614:622	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	5	46	theme	gel	836:838	arg1	strength					840:847	gel strength	836:847	gel strength	836:847	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	3	47	theme	weight	441:446	arg1	ratio					457:461	mixing weight fraction ratio	434:461	mixing weight fraction ratio	434:461	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	5	48	theme	Strongest	775:783	arg1	hydrogels					795:803	Strongest composite hydrogels	775:803	Strongest composite hydrogels	775:803	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	7	49	theme	maximum	1026:1032	arg1	capacity					1048:1055	maximum encapsulation capacity	1026:1055	maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry	1026:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	4	50	theme	hydrogels	694:702	arg1	elasticity					676:685	the elasticity	672:685	the elasticity of the hydrogels	672:702	At acidic pH, a higher weight fraction of alginate increased the elasticity of the hydrogels while at basic pH more percentage of chitosan stabilized the hydrogel.
29753183	0	51	theme	hydrogels	48:56	arg1	behavior					12:19	Rheological behavior	0:19	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.	0:139	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	7	52	theme	applicability	1347:1359	arg1	niche					1334:1338	niche	1334:1338	niche of its applicability in research and industry	1334:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	0	53	from	use	109:111	arg1	encapsulation					116:128	encapsulation	116:128	encapsulation of citral	116:138	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	3	54	theme	encapsulation	573:585	arg1	capacity					587:594	encapsulation capacity	573:594	encapsulation capacity	573:594	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	7	55	theme	chitosan	1000:1007	arg1	hydrogels					1009:1017	The pure chitosan hydrogels	991:1017	The pure chitosan hydrogels	991:1017	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	2	56	theme	basic	335:339	arg1	pH					341:342	neutral and basic pH	323:342	pH	341:342	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	7	57	theme	pure	995:998	arg1	hydrogels					1009:1017	The pure chitosan hydrogels	991:1017	The pure chitosan hydrogels	991:1017	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	7	58	theme	encapsulation	1034:1046	arg1	capacity					1048:1055	maximum encapsulation capacity	1026:1055	maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry	1026:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	1	59	with	hydrogels	164:172	arg1	alginate					198:205	sodium alginate	191:205	sodium alginate	191:205	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	5	60	theme	medium	893:898	arg1	pH					873:874	pH	873:874	pH of the synthetic medium	873:898	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	2	61	theme	neutral	323:329	arg1	pH					341:342	neutral and basic pH	323:342	pH	341:342	Chitosan shows gelation at neutral and basic pH while sodium alginate remains in solution form at all pH values.
29753183	7	62	theme	experimental	1237:1248	arg1	conditions					1250:1259	experimental conditions	1237:1259	experimental conditions exploiting physical interactions	1237:1292	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	0	63	from	Characterization	84:99	arg1	encapsulation					116:128	encapsulation	116:128	encapsulation of citral	116:138	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	0	64	dep	behavior	12:19	arg1	Characterization					84:99	Characterization	84:99	Characterization	84:99	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	0	64	dep	behavior	12:19	arg1	use					109:111	its use	105:111	its use in encapsulation of citral	105:138	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	5	65	theme	acidic	822:827	arg1	pH					829:830	acidic pH	822:830	acidic pH	822:830	Strongest composite hydrogels were obtained at acidic pH and gel strength decreased on increasing pH of the synthetic medium.
29753183	3	66	theme	hydrogels	483:491	arg1	array					474:478	an array	471:478	an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity	471:594	By careful adjustment of mixing weight fraction ratio and pH, an array of hydrogels with modulated structural dynamics and viscoelasticity, mechanical strength and encapsulation capacity were obtained.
29753183	7	67	theme	niche	1334:1338	arg1	understanding					1317:1329	better understanding	1310:1329	better understanding of niche of its applicability in research and industry	1310:1384	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	1	68	theme	various	227:233	arg1	values					238:243	various pH values	227:243	various pH values	227:243	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29753183	0	69	theme	chitosan	61:68	arg1	hydrogels					48:56	pH responsive composite hydrogels	24:56	pH responsive composite hydrogels of chitosan and alginate	24:81	Rheological behavior of pH responsive composite hydrogels of chitosan and alginate: Characterization and its use in encapsulation of citral.
29753183	6	70	theme	synthesized	905:915	arg1	hydrogels					917:925	The synthesized hydrogels	901:925	The synthesized hydrogels	901:925	The synthesized hydrogels were studied for their encapsulation capability towards Citral.
29753183	7	71	from	changes	1226:1232	arg1	conditions					1250:1259	experimental conditions	1237:1259	experimental conditions exploiting physical interactions	1237:1292	The pure chitosan hydrogels showed maximum encapsulation capacity followed by the composite hydrogels prepared at neutral pH. This study highlights that a range of composite hydrogels with modified properties can be obtained by simple changes in experimental conditions exploiting physical interactions and can help in better understanding of niche of its applicability in research and industry.
29753183	1	72	theme	pH	235:236	arg1	values					238:243	various pH values	227:243	various pH values	227:243	The pure and composite hydrogels of chitosan with sodium alginate were synthesized at various pH values and characterized by rheology and IR-spectroscopy.
29707794	6	0	theme	stable	977:982	arg1	structure					984:992	the stable structure	973:992	the stable structure of the films	973:1005	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	4	1	theme	water	592:596	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	1	theme	water	592:596	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	2	2	theme	composite	358:366	arg1	films					368:372	corn distarch phosphate (CDP)-based composite films	322:372	corn distarch phosphate (CDP)-based composite films	322:372	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films was investigated.
29707794	1	3	theme	ultrasonic/microwave-assisted	243:271	arg1	treatment					273:281	ultrasonic/microwave-assisted treatment	243:281	ultrasonic/microwave-assisted treatment	243:281	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	0	4	theme	microcry	98:105	arg1	cellulose					127:135	modified microcry stalline corn straw cellulose	89:135	modified microcry stalline corn straw cellulose	89:135	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	7	5	theme	composite	1260:1268	arg1	films					1270:1274	composite films	1260:1274	composite films	1260:1274	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	3	6	from	break	520:524	arg1	elongation					506:515	the elongation	502:515	the elongation at break	502:524	RESULTS As the proportion of MMCSC was increased, tensile strength increased initially before decreasing, and the elongation at break always decreased.
29707794	2	7	theme	MMCSC	311:315	arg1	incorporation					294:306	Effective incorporation	284:306	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films	284:372	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films was investigated.
29707794	8	8	theme	MMCSC	1318:1322	arg1	incorporation					1292:1304	The incorporation	1288:1304	The incorporation of suitable MMCSC	1288:1322	CONCLUSION The incorporation of suitable MMCSC could improve the properties of composite films.
29707794	0	9	theme	modified	89:96	arg1	cellulose					127:135	modified microcry stalline corn straw cellulose	89:135	modified microcry stalline corn straw cellulose	89:135	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	7	10	theme	scanning	1081:1088	arg1	microscopy					1099:1108	A scanning electron microscopy	1079:1108	A scanning electron microscopy study	1079:1114	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	1	11	dep	BACKGROUND	138:147	arg1	prepared					231:238	prepared	231:238	was prepared by ultrasonic/microwave-assisted treatment	227:281	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	10	12	theme	Chemical	1528:1535	arg1	Industry					1537:1544	Chemical Industry	1528:1544	Chemical Industry	1528:1544	© 2018 Society of Chemical Industry.
29707794	4	13	theme	vapor	598:602	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	13	theme	vapor	598:602	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	0	14	theme	corn	116:119	arg1	cellulose					127:135	modified microcry stalline corn straw cellulose	89:135	modified microcry stalline corn straw cellulose	89:135	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	5	15	theme	*	833:833	arg1	values					835:840	a* values	832:840	a* values	832:840	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	5	16	theme	total	785:789	arg1	difference					797:806	the total color difference	781:806	the total color difference (ΔE* )	781:813	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	5	16	theme	total	785:789	arg1	*					811:811	ΔE*	809:811	ΔE*	809:811	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	4	17	theme	g	631:631	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	17	theme	g	631:631	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	3	18	theme	tensile	442:448	arg1	strength					450:457	tensile strength	442:457	tensile strength	442:457	RESULTS As the proportion of MMCSC was increased, tensile strength increased initially before decreasing, and the elongation at break always decreased.
29707794	0	19	theme	stalline	107:114	arg1	cellulose					127:135	modified microcry stalline corn straw cellulose	89:135	modified microcry stalline corn straw cellulose	89:135	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	6	20	theme	diffraction	903:913	arg1	analysis					915:922	X-ray diffraction analysis	897:922	X-ray diffraction analysis	897:922	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	2	21	theme	Effective	284:292	arg1	incorporation					294:306	Effective incorporation	284:306	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films	284:372	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films was investigated.
29707794	1	22	theme	novel	166:170	arg1	MMCSC					220:224	MMCSC	220:224	MMCSC	220:224	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	1	22	theme	novel	166:170	arg1	cellulose					209:217	a novel modified microcrystalline corn straw cellulose	164:217	a novel modified microcrystalline corn straw cellulose (MMCSC)	164:225	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	5	23	theme	b	767:767	arg1	*					768:768	b*	767:768	b*	767:768	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	8	24	theme	composite	1356:1364	arg1	films					1366:1370	composite films	1356:1370	composite films	1356:1370	CONCLUSION The incorporation of suitable MMCSC could improve the properties of composite films.
29707794	7	25	theme	distinct	1223:1230	arg1	separation					1232:1241	no distinct separation	1220:1241	no distinct separation	1220:1241	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	8	26	theme	suitable	1309:1316	arg1	MMCSC					1318:1322	suitable MMCSC	1309:1322	suitable MMCSC	1309:1322	CONCLUSION The incorporation of suitable MMCSC could improve the properties of composite films.
29707794	1	27	theme	modified	172:179	arg1	MMCSC					220:224	MMCSC	220:224	MMCSC	220:224	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	1	27	theme	modified	172:179	arg1	cellulose					209:217	a novel modified microcrystalline corn straw cellulose	164:217	a novel modified microcrystalline corn straw cellulose (MMCSC)	164:225	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	0	28	theme	Mechanical	0:9	arg1	properties					32:41	Mechanical and moisture barrier properties	0:41	properties	32:41	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	4	29	theme	MMCSC20	566:572	arg1	film					558:561	The composite film	544:561	The composite film of MMCSC20	544:572	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	5	30	dep	*	761:761	arg1	the					756:758	the	756:758	the	756:758	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	1	31	theme	microcrystalline	181:196	arg1	MMCSC					220:224	MMCSC	220:224	MMCSC	220:224	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	1	31	theme	microcrystalline	181:196	arg1	cellulose					209:217	a novel modified microcrystalline corn straw cellulose	164:217	a novel modified microcrystalline corn straw cellulose (MMCSC)	164:225	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	0	32	theme	barrier	24:30	arg1	properties					32:41	Mechanical and moisture barrier properties	0:41	properties	32:41	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	5	33	theme	*	768:768	arg1	values					770:775	the L* and b* values	756:775	the L* and b* values	756:775	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	9	34	contain	have	1457:1460	arg2	potential					1469:1477	a high potential	1462:1477	a high potential to be used for food packaging	1462:1507	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	34	contain	have	1457:1460	arg1	biodegradable					1415:1427	biodegradable	1415:1427	biodegradable	1415:1427	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	34	contain	have	1457:1460	arg1	films					1387:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	34	contain	have	1457:1460	arg1	friendly					1447:1454	friendly	1447:1454	friendly	1447:1454	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	35	theme	CDP-MMCSC	1377:1385	arg1	biodegradable					1415:1427	biodegradable	1415:1427	biodegradable	1415:1427	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	35	theme	CDP-MMCSC	1377:1385	arg1	films					1387:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	35	theme	CDP-MMCSC	1377:1385	arg1	friendly					1447:1454	friendly	1447:1454	friendly	1447:1454	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	5	36	theme	*	761:761	arg1	values					770:775	the L* and b* values	756:775	the L* and b* values	756:775	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	1	37	theme	corn	198:201	arg1	MMCSC					220:224	MMCSC	220:224	MMCSC	220:224	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	1	37	theme	corn	198:201	arg1	cellulose					209:217	a novel modified microcrystalline corn straw cellulose	164:217	a novel modified microcrystalline corn straw cellulose (MMCSC)	164:225	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	0	38	theme	moisture	15:22	arg1	properties					32:41	Mechanical and moisture barrier properties	0:41	properties	32:41	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	6	39	theme	X-ray	897:901	arg1	analysis					915:922	X-ray diffraction analysis	897:922	X-ray diffraction analysis	897:922	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	0	40	theme	straw	121:125	arg1	cellulose					127:135	modified microcry stalline corn straw cellulose	89:135	modified microcry stalline corn straw cellulose	89:135	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	6	41	dep	transform	861:869	arg1	infrared					871:878	infrared	871:878	transform infrared spectroscopy and X-ray diffraction analysis	861:922	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	5	42	theme	a	832:832	arg1	values					835:840	a* values	832:840	a* values	832:840	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	7	43	theme	electron	1090:1097	arg1	microscopy					1099:1108	A scanning electron microscopy	1079:1108	A scanning electron microscopy study	1079:1114	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	1	44	theme	straw	203:207	arg1	MMCSC					220:224	MMCSC	220:224	MMCSC	220:224	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	1	44	theme	straw	203:207	arg1	cellulose					209:217	a novel modified microcrystalline corn straw cellulose	164:217	a novel modified microcrystalline corn straw cellulose (MMCSC)	164:225	BACKGROUND In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment.
29707794	3	45	theme	MMCSC	421:425	arg1	proportion					407:416	the proportion	403:416	the proportion of MMCSC	403:425	RESULTS As the proportion of MMCSC was increased, tensile strength increased initially before decreasing, and the elongation at break always decreased.
29707794	5	46	from	proportion	725:734	arg1	films					749:753	composite films	739:753	composite films	739:753	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	5	47	theme	color	791:795	arg1	difference					797:806	the total color difference	781:806	the total color difference (ΔE* )	781:813	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	5	47	theme	color	791:795	arg1	*					811:811	ΔE*	809:811	ΔE*	809:811	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	5	48	theme	L	760:760	arg1	*					761:761	L*	760:761	L*	760:761	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	4	49	theme	lowest	585:590	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	49	theme	lowest	585:590	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	7	50	theme	microscopy	1099:1108	arg1	study					1110:1114	A scanning electron microscopy study	1079:1114	A scanning electron microscopy study	1079:1114	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	4	51	theme	h-1	637:639	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	51	theme	h-1	637:639	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	0	52	theme	distarch	51:58	arg1	film					70:73	corn distarch phosphate film	46:73	corn distarch phosphate film	46:73	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	6	53	dep	Fourier	853:859	arg1	transform					861:869	transform	861:869	transform infrared spectroscopy and X-ray diffraction analysis	861:922	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	5	54	theme	composite	739:747	arg1	films					749:753	composite films	739:753	composite films	739:753	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	2	55	theme	-based	351:356	arg1	films					368:372	corn distarch phosphate (CDP)-based composite films	322:372	corn distarch phosphate (CDP)-based composite films	322:372	Effective incorporation of MMCSC into corn distarch phosphate (CDP)-based composite films was investigated.
29707794	0	56	theme	corn	46:49	arg1	film					70:73	corn distarch phosphate film	46:73	corn distarch phosphate film	46:73	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	5	57	theme	surface	668:674	arg1	color					676:680	surface color	668:680	surface color	668:680	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	7	58	theme	films	1270:1274	arg1	matrix					1250:1255	the matrix	1246:1255	the matrix of composite films	1246:1274	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	4	59	theme	composite	548:556	arg1	film					558:561	The composite film	544:561	The composite film of MMCSC20	544:572	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	5	60	theme	color	676:680	arg1	measurement					653:663	The measurement	649:663	The measurement of surface color	649:680	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	9	61	dep	biodegradable	1415:1427	arg1	biodegradable					1415:1427	biodegradable	1415:1427	biodegradable	1415:1427	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	61	dep	biodegradable	1415:1427	arg1	friendly					1447:1454	friendly	1447:1454	friendly	1447:1454	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	9	61	dep	biodegradable	1415:1427	arg1	films					1387:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films	1373:1391	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	8	62	theme	films	1366:1370	arg1	properties					1342:1351	the properties	1338:1351	the properties of composite films	1338:1370	CONCLUSION The incorporation of suitable MMCSC could improve the properties of composite films.
29707794	8	63	dep	CONCLUSION	1277:1286	arg1	improve					1330:1336	improve	1330:1336	could improve the properties of composite films	1324:1370	CONCLUSION The incorporation of suitable MMCSC could improve the properties of composite films.
29707794	4	64	theme	2.917	618:622	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	64	theme	2.917	618:622	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	9	65	theme	food	1494:1497	arg1	packaging					1499:1507	food packaging	1494:1507	food packaging	1494:1507	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	7	66	theme	films	1155:1159	arg1	smooth					1182:1187	smooth	1182:1187	smooth	1182:1187	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	7	66	theme	films	1155:1159	arg1	microstructure					1137:1150	the surface microstructure	1125:1150	the surface microstructure of films (MMCSC0-MMCSC30)	1125:1176	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	9	67	theme	high	1464:1467	arg1	potential					1469:1477	a high potential	1462:1477	a high potential to be used for food packaging	1462:1507	The CDP-MMCSC films, which are completely biodegradable and environmental friendly, have a high potential to be used for food packaging.
29707794	0	68	theme	film	70:73	arg1	properties					32:41	Mechanical and moisture barrier properties	0:41	properties	32:41	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	5	69	theme	MMCSC	719:723	arg1	proportion					725:734	the MMCSC proportion	715:734	the MMCSC proportion in composite films	715:753	The measurement of surface color showed that by the increasing of the MMCSC proportion in composite films, the L* and b* values and the total color difference (ΔE* ) increased, while a* values decreased.
29707794	4	70	theme	m-1	633:635	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	70	theme	m-1	633:635	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	6	71	theme	films	1001:1005	arg1	structure					984:992	the stable structure	973:992	the stable structure of the films	973:1005	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	0	72	theme	phosphate	60:68	arg1	film					70:73	corn distarch phosphate film	46:73	corn distarch phosphate film	46:73	Mechanical and moisture barrier properties of corn distarch phosphate film influenced by modified microcry stalline corn straw cellulose.
29707794	7	73	theme	surface	1129:1135	arg1	smooth					1182:1187	smooth	1182:1187	smooth	1182:1187	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	7	73	theme	surface	1129:1135	arg1	microstructure					1137:1150	the surface microstructure	1125:1150	the surface microstructure of films (MMCSC0-MMCSC30)	1125:1176	A scanning electron microscopy study revealed the surface microstructure of films (MMCSC0-MMCSC30) was smooth and homogeneous, and there was no distinct separation in the matrix of composite films.
29707794	6	74	theme	MMCSC	966:970	arg1	incorporation					949:961	the incorporation	945:961	the incorporation of MMCSC	945:970	Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that, with the incorporation of MMCSC, the stable structure of the films was enhanced through cross-linking and the crystallinity was increased.
29707794	4	75	theme	×	624:624	arg1	permeability					604:615	water vapor permeability	592:615	the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 )	581:646	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
29707794	4	75	theme	×	624:624	arg1	Pa-1					641:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	2.917 × 10-7 g m-1 h-1 Pa-1	618:644	The composite film of MMCSC20 showed the lowest water vapor permeability (2.917 × 10-7 g m-1 h-1 Pa-1 ).
31546290	6	0	theme	electron	962:969	arg1	microscopy					971:980	scanning electron microscopy	953:980	scanning electron microscopy	953:980	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	10	1	theme	composition	1648:1658	arg1	type					1617:1620	the type	1613:1620	the type of drying method and wall composition	1613:1658	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	11	2	theme	relative	1745:1752	arg1	humidities					1754:1763	relative humidities	1745:1763	relative humidities	1745:1763	To assess the shelf life, the microcapsules were kept at different temperatures and relative humidities for 16 weeks.
31546290	7	3	theme	polyphenolic	1203:1214	arg1	compounds					1216:1224	the polyphenolic compounds	1199:1224	the polyphenolic compounds of the microsphere	1199:1243	High-performance liquid chromatography was used to evaluate the polyphenolic compounds of the microsphere.
31546290	14	4	theme	compounds	2502:2510	arg1	release					2491:2497	controlled release	2480:2497	controlled release of compounds in the food and pharmaceutical industries	2480:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	14	4	theme	compounds	2502:2510	arg1	release					2312:2318	the release	2308:2318	the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2308:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	12	5	theme	antioxidant	2091:2101	arg1	compounds					2103:2111	various antioxidant compounds	2083:2111	various antioxidant compounds	2083:2111	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	14	6	from	release	2491:2497	arg1	industries					2543:2552	the food and pharmaceutical industries	2515:2552	the food and pharmaceutical industries	2515:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	14	6	from	release	2491:2497	arg1	system					2365:2370	the simulated system	2351:2370	the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2351:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	14	7	theme	microencapsulated	2323:2339	arg1	powder					2341:2346	microencapsulated powder	2323:2346	microencapsulated powder	2323:2346	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	3	8	from	increase	498:505	arg1	composition					535:545	wall composition	530:545	wall composition	530:545	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	4	9	from	capacity	677:684	arg1	samples					706:712	pectin-contained samples	689:712	pectin-contained samples	689:712	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	7	10	used	used	1182:1185	arg2	chromatography					1163:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography was used to evaluate the polyphenolic compounds of the microsphere.
31546290	3	11	theme	pectin	514:519	arg1	ratio					521:525	the pectin ratio	510:525	the pectin ratio	510:525	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	2	12	theme	methods	291:297	arg1	effect					274:279	the effect	270:279	the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin)	270:372	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	5	13	theme	freeze	925:930	arg1	method					939:944	freeze drying method	925:944	freeze drying method	925:944	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	6	14	theme	scanning	1019:1026	arg1	calorimetry					1028:1038	differential scanning calorimetry	1006:1038	differential scanning calorimetry	1006:1038	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	8	15	theme	petal	1366:1370	arg1	extract					1372:1378	saffron petal extract	1358:1378	saffron petal extract	1358:1378	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	7	16	theme	High-performance	1139:1154	arg1	chromatography					1163:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography was used to evaluate the polyphenolic compounds of the microsphere.
31546290	8	17	theme	high	1315:1318	arg1	levels					1320:1325	high levels	1315:1325	high levels of routine	1315:1336	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	12	18	from	levels	1843:1848	arg1	composition					1868:1878	wall composition	1863:1878	wall composition	1863:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	4	19	theme	Microencapsulation	635:652	arg1	efficiency					654:663	Microencapsulation efficiency	635:663	Microencapsulation efficiency	635:663	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	13	20	theme	increased	2251:2259	arg1	bioavailability					2261:2275	their increased bioavailability	2245:2275	their increased bioavailability	2245:2275	Microencapsulation of its valuable compounds results in preventing the destruction of these compounds by environmental factors and their increased bioavailability.
31546290	3	21	theme	polyphenols	572:582	arg1	content					584:590	polyphenols content	572:590	polyphenols content	572:590	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	1	22	theme	considerable	202:213	arg1	amount					215:220	a considerable amount	200:220	a considerable amount of antioxidant compounds	200:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	1	22	theme	considerable	202:213	arg1	compounds					237:245	antioxidant compounds	225:245	antioxidant compounds	225:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	13	23	theme	compounds	2206:2214	arg1	destruction					2185:2195	the destruction	2181:2195	the destruction of these compounds by environmental factors and their increased bioavailability	2181:2275	Microencapsulation of its valuable compounds results in preventing the destruction of these compounds by environmental factors and their increased bioavailability.
31546290	5	24	contain	had	816:818	arg2	capacity					869:876	loading capacity	861:876	loading capacity	861:876	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	5	24	contain	had	816:818	arg2	efficiency					846:855	higher microencapsulation efficiency	820:855	higher microencapsulation efficiency	820:855	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	5	24	contain	had	816:818	arg1	microcapsules					768:780	microcapsules	768:780	microcapsules obtained from spray drying method	768:814	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	4	25	theme	maltodextrin	736:747	arg1	samples					749:755	pure maltodextrin samples	731:755	pure maltodextrin samples	731:755	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	14	26	theme	final	2443:2447	arg1	product					2449:2455	the final product	2439:2455	the final product	2439:2455	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	13	27	theme	environmental	2219:2231	arg1	factors					2233:2239	environmental factors	2219:2239	environmental factors	2219:2239	Microencapsulation of its valuable compounds results in preventing the destruction of these compounds by environmental factors and their increased bioavailability.
31546290	3	28	theme	activity	608:615	arg1	increase					560:567	the increase	556:567	the increase of polyphenols content and antioxidant activity of microcapsules	556:632	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	4	29	theme	pectin-contained	689:704	arg1	samples					706:712	pectin-contained samples	689:712	pectin-contained samples	689:712	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	5	30	theme	loading	861:867	arg1	capacity					869:876	loading capacity	861:876	loading capacity	861:876	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	14	31	theme	shelf	2425:2429	arg1	life					2431:2434	the shelf life	2421:2434	the shelf life of the final product	2421:2455	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	0	32	theme	In	78:79	arg1	Digestion					104:112	In Vitro Gastrointestinal Digestion	78:112	In Vitro Gastrointestinal Digestion	78:112	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	14	33	from	release	2312:2318	arg1	industries					2543:2552	the food and pharmaceutical industries	2515:2552	the food and pharmaceutical industries	2515:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	14	33	from	release	2312:2318	arg1	system					2365:2370	the simulated system	2351:2370	the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2351:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	10	34	theme	release	1555:1561	arg1	behavior					1563:1570	the release behavior	1551:1570	the release behavior of the microcapsules	1551:1591	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	0	35	theme	Gastrointestinal	87:102	arg1	Digestion					104:112	In Vitro Gastrointestinal Digestion	78:112	In Vitro Gastrointestinal Digestion	78:112	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	13	36	theme	compounds	2149:2157	arg1	Microencapsulation					2114:2131	Microencapsulation	2114:2131	Microencapsulation of its valuable compounds	2114:2157	Microencapsulation of its valuable compounds results in preventing the destruction of these compounds by environmental factors and their increased bioavailability.
31546290	12	37	theme	huge	2024:2027	arg1	by-product					2047:2056	saffron by-product	2039:2056	saffron by-product	2039:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	37	theme	huge	2024:2027	arg1	Saffron					2003:2009	Saffron petal	2003:2015	Saffron petal	2003:2015	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	37	theme	huge	2024:2027	arg1	amount					2029:2034	the huge amount	2020:2034	the huge amount of saffron by-product	2020:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	2	38	dep	methods	291:297	arg1	freeze					310:315	freeze	310:315	freeze	310:315	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	38	dep	methods	291:297	arg1	spray					300:304	spray	300:304	spray	300:304	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	38	dep	methods	291:297	arg1	methods					291:297	drying methods	284:297	drying methods (spray and freeze)	284:316	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	39	dep	structures	337:346	arg1	pectin					366:371	pectin	366:371	pectin	366:371	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	39	dep	structures	337:346	arg1	maltodextrin					349:360	maltodextrin	349:360	maltodextrin	349:360	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	39	dep	structures	337:346	arg1	structures					337:346	different wall structures	322:346	different wall structures (maltodextrin and pectin)	322:372	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	9	40	theme	polyphenols	1409:1419	arg1	release					1398:1404	the release	1394:1404	the release of polyphenols from microcapsules of saffron petal extract	1394:1463	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	5	41	theme	drying	802:807	arg1	method					809:814	spray drying method	796:814	spray drying method	796:814	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	10	42	theme	microcapsules	1579:1591	arg1	behavior					1563:1570	the release behavior	1551:1570	the release behavior of the microcapsules	1551:1591	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	0	43	dep	In	78:79	arg1	Vitro					81:85	Vitro	81:85	Vitro	81:85	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	6	44	theme	produced	1115:1122	arg1	microcapsules					1124:1136	produced microcapsules	1115:1136	produced microcapsules	1115:1136	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	2	45	theme	physicochemical	398:412	arg1	characteristics					414:428	the physicochemical characteristics	394:428	the physicochemical characteristics of microcapsules of saffron petal extracts	394:471	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	12	46	contain	contains	2062:2069	arg1	Saffron					2003:2009	Saffron petal	2003:2015	Saffron petal	2003:2015	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	46	contain	contains	2062:2069	arg1	by-product					2047:2056	saffron by-product	2039:2056	saffron by-product	2039:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	46	contain	contains	2062:2069	arg2	number					2073:2078	a number	2071:2078	a number of various antioxidant compounds	2071:2111	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	46	contain	contains	2062:2069	arg1	amount					2029:2034	the huge amount	2020:2034	the huge amount of saffron by-product	2020:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	5	47	with	comparison	881:890	arg1	microcapsules					897:909	microcapsules	897:909	microcapsules obtained from freeze drying method	897:944	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	14	48	theme	digestive	2379:2387	arg1	system					2389:2394	the digestive system	2375:2394	the digestive system that helps us to improve the shelf life of the final product during the process	2375:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	12	49	theme	°C.	1976:1978	arg1	APPLICATION					1990:2000	4 °C. PRACTICAL APPLICATION	1974:2000	4 °C. PRACTICAL APPLICATION	1974:2000	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	0	50	theme	Saffron	22:28	arg1	Extract					45:51	Saffron Petal Phenolic Extract	22:51	Saffron Petal Phenolic Extract	22:51	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	9	51	theme	saffron	1443:1449	arg1	extract					1457:1463	saffron petal extract	1443:1463	saffron petal extract	1443:1463	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	0	52	dep	Characterization	60:75	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of Saffron Petal Phenolic Extract	0:51	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	5	53	theme	drying	932:937	arg1	method					939:944	freeze drying method	925:944	freeze drying method	925:944	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	2	54	theme	petal	458:462	arg1	extracts					464:471	saffron petal extracts	450:471	saffron petal extracts	450:471	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	7	55	theme	microsphere	1233:1243	arg1	compounds					1216:1224	the polyphenolic compounds	1199:1224	the polyphenolic compounds of the microsphere	1199:1243	High-performance liquid chromatography was used to evaluate the polyphenolic compounds of the microsphere.
31546290	9	56	theme	extract	1457:1463	arg1	microcapsules					1426:1438	microcapsules	1426:1438	microcapsules of saffron petal extract	1426:1463	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	0	57	theme	Phenolic	36:43	arg1	Extract					45:51	Saffron Petal Phenolic Extract	22:51	Saffron Petal Phenolic Extract	22:51	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	12	58	theme	relative	1930:1937	arg1	humidity					1939:1946	relative humidity	1930:1946	relative humidity of 11%	1930:1953	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	10	59	theme	wall	1643:1646	arg1	composition					1648:1658	wall composition	1643:1658	wall composition	1643:1658	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	3	60	theme	microcapsules	620:632	arg1	content					584:590	polyphenols content	572:590	polyphenols content	572:590	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	3	60	theme	microcapsules	620:632	arg1	activity					608:615	antioxidant activity	596:615	antioxidant activity of microcapsules	596:632	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	5	61	theme	higher	820:825	arg1	efficiency					846:855	higher microencapsulation efficiency	820:855	higher microencapsulation efficiency	820:855	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	10	62	theme	method	1632:1637	arg1	type					1617:1620	the type	1613:1620	the type of drying method and wall composition	1613:1658	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	2	63	theme	present	255:261	arg1	study					263:267	the present study	251:267	the present study	251:267	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	13	64	theme	valuable	2140:2147	arg1	compounds					2149:2157	its valuable compounds	2136:2157	its valuable compounds	2136:2157	Microencapsulation of its valuable compounds results in preventing the destruction of these compounds by environmental factors and their increased bioavailability.
31546290	9	65	theme	simulated	1485:1493	arg1	conditions					1512:1521	simulated gastrointestinal conditions	1485:1521	simulated gastrointestinal conditions	1485:1521	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	14	66	theme	food	2519:2522	arg1	industries					2543:2552	the food and pharmaceutical industries	2515:2552	the food and pharmaceutical industries	2515:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	2	67	theme	structures	337:346	arg1	effect					274:279	the effect	270:279	the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin)	270:372	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	1	68	theme	antioxidant	225:235	arg1	compounds					237:245	antioxidant compounds	225:245	antioxidant compounds	225:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	11	69	theme	different	1718:1726	arg1	temperatures					1728:1739	different temperatures	1718:1739	different temperatures	1718:1739	To assess the shelf life, the microcapsules were kept at different temperatures and relative humidities for 16 weeks.
31546290	2	70	theme	different	322:330	arg1	pectin					366:371	pectin	366:371	pectin	366:371	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	70	theme	different	322:330	arg1	maltodextrin					349:360	maltodextrin	349:360	maltodextrin	349:360	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	70	theme	different	322:330	arg1	structures					337:346	different wall structures	322:346	different wall structures (maltodextrin and pectin)	322:372	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	6	71	theme	scanning	953:960	arg1	microscopy					971:980	scanning electron microscopy	953:980	scanning electron microscopy	953:980	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	14	72	theme	powder	2341:2346	arg1	release					2491:2497	controlled release	2480:2497	controlled release of compounds in the food and pharmaceutical industries	2480:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	14	72	theme	powder	2341:2346	arg1	release					2312:2318	the release	2308:2318	the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2308:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	3	73	theme	ratio	521:525	arg1	increase					498:505	the increase	494:505	the increase of the pectin ratio in wall composition	494:545	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	12	74	theme	various	2083:2089	arg1	compounds					2103:2111	various antioxidant compounds	2083:2111	various antioxidant compounds	2083:2111	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	75	theme	highest	1888:1894	arg1	activity					1908:1915	the highest antioxidant activity	1884:1915	the highest antioxidant activity	1884:1915	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	14	76	theme	controlled	2480:2489	arg1	release					2491:2497	controlled release	2480:2497	controlled release of compounds in the food and pharmaceutical industries	2480:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	12	77	theme	compounds	2103:2111	arg1	number					2073:2078	a number	2071:2078	a number of various antioxidant compounds	2071:2111	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	78	theme	pectin	1853:1858	arg1	levels					1843:1848	high levels	1838:1848	high levels of pectin in wall composition	1838:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	8	79	theme	routine	1330:1336	arg1	levels					1320:1325	high levels	1315:1325	high levels of routine	1315:1336	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	5	80	theme	spray	796:800	arg1	method					809:814	spray drying method	796:814	spray drying method	796:814	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	7	81	theme	liquid	1156:1161	arg1	chromatography					1163:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography	1139:1176	High-performance liquid chromatography was used to evaluate the polyphenolic compounds of the microsphere.
31546290	4	82	from	efficiency	654:663	arg1	samples					706:712	pectin-contained samples	689:712	pectin-contained samples	689:712	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	1	83	theme	saffron	171:177	arg1	processing					179:188	saffron processing	171:188	saffron processing	171:188	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	12	84	theme	wall	1863:1866	arg1	composition					1868:1878	wall composition	1863:1878	wall composition	1863:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	4	85	theme	loading	669:675	arg1	capacity					677:684	loading capacity	669:684	loading capacity	669:684	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	11	86	theme	shelf	1675:1679	arg1	life					1681:1684	the shelf life	1671:1684	the shelf life	1671:1684	To assess the shelf life, the microcapsules were kept at different temperatures and relative humidities for 16 weeks.
31546290	2	87	theme	drying	284:289	arg1	freeze					310:315	freeze	310:315	freeze	310:315	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	87	theme	drying	284:289	arg1	spray					300:304	spray	300:304	spray	300:304	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	87	theme	drying	284:289	arg1	methods					291:297	drying methods	284:297	drying methods (spray and freeze)	284:316	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	8	88	theme	encapsulation	1283:1295	arg1	methods					1297:1303	both encapsulation methods	1278:1303	both encapsulation methods	1278:1303	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	6	89	theme	differential	1006:1017	arg1	calorimetry					1028:1038	differential scanning calorimetry	1006:1038	differential scanning calorimetry	1006:1038	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	8	90	theme	saffron	1358:1364	arg1	extract					1372:1378	saffron petal extract	1358:1378	saffron petal extract	1358:1378	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	1	91	theme	processing	179:188	arg1	byproduct					158:166	a byproduct	156:166	a byproduct of saffron processing	156:188	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	8	92	theme	extract	1372:1378	arg1	microcapsules					1341:1353	microcapsules	1341:1353	microcapsules of saffron petal extract	1341:1378	The chromatograms obtained from both encapsulation methods indicated high levels of routine in microcapsules of saffron petal extract.
31546290	12	93	theme	by-product	2047:2056	arg1	by-product					2047:2056	saffron by-product	2039:2056	saffron by-product	2039:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	93	theme	by-product	2047:2056	arg1	Saffron					2003:2009	Saffron petal	2003:2015	Saffron petal	2003:2015	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	93	theme	by-product	2047:2056	arg1	amount					2029:2034	the huge amount	2020:2034	the huge amount of saffron by-product	2020:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	6	94	theme	X-ray	983:987	arg1	diffraction					989:999	X-ray diffraction	983:999	X-ray diffraction	983:999	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	14	95	theme	product	2449:2455	arg1	life					2431:2434	the shelf life	2421:2434	the shelf life of the final product	2421:2455	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	3	96	theme	antioxidant	596:606	arg1	activity					608:615	antioxidant activity	596:615	antioxidant activity of microcapsules	596:632	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	3	97	theme	wall	530:533	arg1	composition					535:545	wall composition	530:545	wall composition	530:545	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	14	98	theme	pharmaceutical	2528:2541	arg1	industries					2543:2552	the food and pharmaceutical industries	2515:2552	the food and pharmaceutical industries	2515:2552	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	4	99	theme	pure	731:734	arg1	samples					749:755	pure maltodextrin samples	731:755	pure maltodextrin samples	731:755	Microencapsulation efficiency and loading capacity in pectin-contained samples were higher than pure maltodextrin samples.
31546290	3	100	theme	content	584:590	arg1	increase					560:567	the increase	556:567	the increase of polyphenols content and antioxidant activity of microcapsules	556:632	Results showed that the increase of the pectin ratio in wall composition leads to the increase of polyphenols content and antioxidant activity of microcapsules.
31546290	1	101	theme	petal	146:150	arg1	Saffron					138:144	Saffron petal	138:150	Saffron petal	138:150	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	12	102	theme	high	1838:1841	arg1	levels					1843:1848	high levels	1838:1848	high levels of pectin in wall composition	1838:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	6	103	theme	useful	1062:1067	arg1	tools					1069:1073	useful tools	1062:1073	useful tools for establishing the difference between produced microcapsules	1062:1136	Also, scanning electron microscopy, X-ray diffraction, and differential scanning calorimetry have been shown to be useful tools for establishing the difference between produced microcapsules.
31546290	2	104	theme	extracts	464:471	arg1	microcapsules					433:445	microcapsules	433:445	microcapsules of saffron petal extracts	433:471	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	12	105	theme	freeze	1809:1814	arg1	drying					1816:1821	freeze drying	1809:1821	freeze drying	1809:1821	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	106	contain	had	1880:1882	arg2	activity					1908:1915	the highest antioxidant activity	1884:1915	the highest antioxidant activity	1884:1915	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	106	contain	had	1880:1882	arg1	microcapsules					1783:1795	The microcapsules	1779:1795	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition	1779:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	107	theme	saffron	2039:2045	arg1	by-product					2047:2056	saffron by-product	2039:2056	saffron by-product	2039:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	5	108	theme	microencapsulation	827:844	arg1	efficiency					846:855	higher microencapsulation efficiency	820:855	higher microencapsulation efficiency	820:855	Moreover, microcapsules obtained from spray drying method had higher microencapsulation efficiency and loading capacity in comparison with microcapsules obtained from freeze drying method.
31546290	9	109	from	microcapsules	1426:1438	arg1	release					1398:1404	the release	1394:1404	the release of polyphenols from microcapsules of saffron petal extract	1394:1463	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	14	110	theme	system	2389:2394	arg1	system					2365:2370	the simulated system	2351:2370	the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2351:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	0	111	theme	Petal	30:34	arg1	Extract					45:51	Saffron Petal Phenolic Extract	22:51	Saffron Petal Phenolic Extract	22:51	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	12	112	theme	petal	2011:2015	arg1	Saffron					2003:2009	Saffron petal	2003:2015	Saffron petal	2003:2015	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	112	theme	petal	2011:2015	arg1	by-product					2047:2056	saffron by-product	2039:2056	saffron by-product	2039:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	112	theme	petal	2011:2015	arg1	amount					2029:2034	the huge amount	2020:2034	the huge amount of saffron by-product	2020:2056	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	0	113	theme	Extract	45:51	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of Saffron Petal Phenolic Extract	0:51	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	9	114	theme	petal	1451:1455	arg1	extract					1457:1463	saffron petal extract	1443:1463	saffron petal extract	1443:1463	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	12	115	theme	PRACTICAL	1980:1988	arg1	APPLICATION					1990:2000	4 °C. PRACTICAL APPLICATION	1974:2000	4 °C. PRACTICAL APPLICATION	1974:2000	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	2	116	theme	saffron	450:456	arg1	extracts					464:471	saffron petal extracts	450:471	saffron petal extracts	450:471	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	12	117	theme	%	1953:1953	arg1	temperature					1959:1969	temperature	1959:1969	temperature of 4 °C. PRACTICAL APPLICATION	1959:2000	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	117	theme	%	1953:1953	arg1	humidity					1939:1946	relative humidity	1930:1946	relative humidity of 11%	1930:1953	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	2	118	theme	microcapsules	433:445	arg1	characteristics					414:428	the physicochemical characteristics	394:428	the physicochemical characteristics of microcapsules of saffron petal extracts	394:471	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	12	119	theme	APPLICATION	1990:2000	arg1	temperature					1959:1969	temperature	1959:1969	temperature of 4 °C. PRACTICAL APPLICATION	1959:2000	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	119	theme	APPLICATION	1990:2000	arg1	humidity					1939:1946	relative humidity	1930:1946	relative humidity of 11%	1930:1953	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	10	120	theme	drying	1625:1630	arg1	method					1632:1637	drying method	1625:1637	drying method	1625:1637	The results indicated that the release behavior of the microcapsules varied according to the type of drying method and wall composition.
31546290	1	121	contain	contains	191:198	arg2	amount					215:220	a considerable amount	200:220	a considerable amount of antioxidant compounds	200:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	1	121	contain	contains	191:198	arg1	Saffron					138:144	Saffron petal	138:150	Saffron petal	138:150	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	1	121	contain	contains	191:198	arg2	compounds					237:245	antioxidant compounds	225:245	antioxidant compounds	225:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	12	122	dep	microcapsules	1783:1795	arg1	containing					1827:1836	containing	1827:1836	containing high levels of pectin in wall composition	1827:1878	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	12	122	dep	microcapsules	1783:1795	arg1	produced					1797:1804	produced	1797:1804	produced by freeze drying	1797:1821	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	9	123	theme	gastrointestinal	1495:1510	arg1	conditions					1512:1521	simulated gastrointestinal conditions	1485:1521	simulated gastrointestinal conditions	1485:1521	In addition, the release of polyphenols from microcapsules of saffron petal extract was evaluated under simulated gastrointestinal conditions.
31546290	12	124	theme	antioxidant	1896:1906	arg1	activity					1908:1915	the highest antioxidant activity	1884:1915	the highest antioxidant activity	1884:1915	The microcapsules produced by freeze drying and containing high levels of pectin in wall composition had the highest antioxidant activity when kept in relative humidity of 11% and temperature of 4 °C. PRACTICAL APPLICATION: Saffron petal is the huge amount of saffron by-product and contains a number of various antioxidant compounds.
31546290	0	125	theme	Storage	119:125	arg1	Stability					127:135	Storage Stability	119:135	Storage Stability	119:135	Microencapsulation of Saffron Petal Phenolic Extract: Their Characterization, In Vitro Gastrointestinal Digestion, and Storage Stability.
31546290	14	126	theme	simulated	2355:2363	arg1	system					2365:2370	the simulated system	2351:2370	the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process	2351:2474	Indeed, this paper focuses on the release of microencapsulated powder in the simulated system of the digestive system that helps us to improve the shelf life of the final product during the process and controlled release of compounds in the food and pharmaceutical industries.
31546290	2	127	theme	wall	332:335	arg1	pectin					366:371	pectin	366:371	pectin	366:371	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	127	theme	wall	332:335	arg1	maltodextrin					349:360	maltodextrin	349:360	maltodextrin	349:360	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	2	127	theme	wall	332:335	arg1	structures					337:346	different wall structures	322:346	different wall structures (maltodextrin and pectin)	322:372	In the present study, the effect of drying methods (spray and freeze) and different wall structures (maltodextrin and pectin) was investigated on the physicochemical characteristics of microcapsules of saffron petal extracts.
31546290	1	128	theme	compounds	237:245	arg1	amount					215:220	a considerable amount	200:220	a considerable amount of antioxidant compounds	200:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31546290	1	128	theme	compounds	237:245	arg1	compounds					237:245	antioxidant compounds	225:245	antioxidant compounds	225:245	Saffron petal, as a byproduct of saffron processing, contains a considerable amount of antioxidant compounds.
31042409	3	0	theme	CSC	321:323	arg1	markers					325:331	CSC markers	321:331	CSC markers	321:331	The correlation between CSC markers and proteoglycan remains to be clarified.
31042409	5	1	theme	gene	729:732	arg1	expression					734:743	CD13 and CD44 gene expression	715:743	expression	734:743	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	1	2	theme	cell	168:171	arg1	differentiation					173:187	cell differentiation	168:187	cell differentiation	168:187	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	11	3	from	Application	1342:1352	arg1	reduction					1375:1383	reduction	1375:1383	reduction of hepatoma stem cells	1375:1406	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	8	4	theme	hepatoma	1123:1130	arg1	formation					1139:1147	hepatoma sphere formation	1123:1147	hepatoma sphere formation	1123:1147	These explained the inhibition of hepatoma sphere formation by exogenous heparin.
31042409	4	5	theme	family	483:488	arg1	SDC1					510:513	SDC1	510:513	SDC1	510:513	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	5	theme	family	483:488	arg1	syndecan-1					498:507	the syndecan family protein syndecan-1	470:507	the syndecan family protein syndecan-1 (SDC1)	470:514	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	5	theme	family	483:488	arg1	markers					425:431	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	11	6	dep	heparin	1357:1363	arg1	mimics					1365:1370	mimics	1365:1370	mimics	1365:1370	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	5	7	theme	Further	547:553	arg1	examination					555:565	Further examination	547:565	Further examination by suppression of CD13 expression	547:599	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	5	8	theme	gene	685:688	arg1	expression					690:699	SDC1 gene expression	680:699	SDC1 gene expression	680:699	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	0	9	from	Role	0:3	arg1	formation					60:68	hepatoma sphere formation	44:68	hepatoma sphere formation	44:68	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	5	10	theme	SDC1	626:629	arg1	expression					645:654	CD44 gene expression	635:654	CD44 gene expression	635:654	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	5	10	theme	SDC1	626:629	arg1	downregulation					608:621	downregulation	608:621	downregulation of SDC1	608:629	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	7	11	theme	disaccharide	914:925	arg1	components					927:936	The heparin disaccharide components	902:936	The heparin disaccharide components	902:936	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	5	12	theme	expression	690:699	arg1	suppression					665:675	suppression	665:675	suppression of SDC1 gene expression	665:699	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	6	13	theme	expression	771:780	arg1	Suppression					746:756	Suppression	746:756	Suppression of SDC1 gene expression	746:780	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	10	14	theme	heparin	1273:1279	arg1	components					1281:1290	SDC1 proteoglycan and heparin components	1251:1290	components	1281:1290	SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development.
31042409	11	15	theme	stem	1397:1400	arg1	cells					1402:1406	hepatoma stem cells	1388:1406	hepatoma stem cells	1388:1406	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	6	16	theme	gene	766:769	arg1	expression					771:780	SDC1 gene expression	761:780	SDC1 gene expression	761:780	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	5	17	theme	CD13	715:718	arg1	expression					734:743	CD13 and CD44 gene expression	715:743	expression	734:743	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	5	18	theme	CD13	585:588	arg1	expression					590:599	CD13 expression	585:599	CD13 expression	585:599	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	6	19	theme	SDC1	761:764	arg1	expression					771:780	SDC1 gene expression	761:780	SDC1 gene expression	761:780	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	1	20	theme	Glycosaminoglycan-modified	71:96	arg1	proteoglycans					98:110	Glycosaminoglycan-modified proteoglycans	71:110	Glycosaminoglycan-modified proteoglycans	71:110	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	1	21	theme	stem	193:196	arg1	development					203:213	stem cell development	193:213	stem cell development	193:213	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	6	22	theme	sphereocrit	841:851	arg1	assay					853:857	a novel sphereocrit assay	833:857	a novel sphereocrit assay to quantify the level of sphere formation	833:899	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	5	23	theme	expression	590:599	arg1	suppression					570:580	suppression	570:580	suppression of CD13 expression	570:599	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	7	24	theme	heparin	906:912	arg1	components					927:936	The heparin disaccharide components	902:936	The heparin disaccharide components	902:936	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	10	25	theme	SDC1	1251:1254	arg1	proteoglycan					1256:1267	SDC1 proteoglycan and heparin components	1251:1290	proteoglycan	1256:1267	SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development.
31042409	1	26	theme	cell	198:201	arg1	development					203:213	stem cell development	193:213	stem cell development	193:213	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	7	27	theme	2-O-sulfation	1074:1086	arg1	levels					1048:1053	the increased levels	1034:1053	the increased levels of N-sulfation and 2-O-sulfation	1034:1086	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	7	28	theme	N-sulfation	1058:1068	arg1	levels					1048:1053	the increased levels	1034:1053	the increased levels of N-sulfation and 2-O-sulfation	1034:1086	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	4	29	theme	sphere	389:394	arg1	formation					396:404	hepatoma sphere formation	380:404	hepatoma sphere formation	380:404	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	30	theme	markers	425:431	arg1	expression					407:416	expression	407:416	expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	407:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	8	31	theme	sphere	1132:1137	arg1	formation					1139:1147	hepatoma sphere formation	1123:1147	hepatoma sphere formation	1123:1147	These explained the inhibition of hepatoma sphere formation by exogenous heparin.
31042409	7	32	theme	increased	1038:1046	arg1	levels					1048:1053	the increased levels	1034:1053	the increased levels of N-sulfation and 2-O-sulfation	1034:1086	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	4	33	theme	hepatoma	380:387	arg1	formation					396:404	hepatoma sphere formation	380:404	hepatoma sphere formation	380:404	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	1	34	theme	important	117:125	arg1	roles					127:131	important roles	117:131	important roles	117:131	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	2	35	from	properties	257:266	arg1	CSCs					290:293	CSCs	290:293	CSCs	290:293	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	35	from	properties	257:266	arg1	cells					283:287	cancer stem cells	271:287	cancer stem cells (CSCs)	271:294	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	0	36	theme	syndecan-1	8:17	arg1	Role					0:3	Role	0:3	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.	0:69	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	6	37	theme	novel	835:839	arg1	assay					853:857	a novel sphereocrit assay	833:857	a novel sphereocrit assay to quantify the level of sphere formation	833:899	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	0	38	theme	heparin	33:39	arg1	Role					0:3	Role	0:3	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.	0:69	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	9	39	theme	CSC	1214:1216	arg1	CD13					1225:1228	CSC marker CD13 and CD44 expression	1214:1248	CD13	1225:1228	In conclusion, we found that SDC1 affected CSC marker CD13 and CD44 expression.
31042409	11	40	theme	hepatoma	1388:1395	arg1	cells					1402:1406	hepatoma stem cells	1388:1406	hepatoma stem cells	1388:1406	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	0	41	theme	exogenous	23:31	arg1	heparin					33:39	exogenous heparin	23:39	exogenous heparin	23:39	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	5	42	theme	SDC1	680:683	arg1	expression					690:699	SDC1 gene expression	680:699	SDC1 gene expression	680:699	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	9	43	theme	marker	1218:1223	arg1	CD13					1225:1228	CSC marker CD13 and CD44 expression	1214:1248	CD13	1225:1228	In conclusion, we found that SDC1 affected CSC marker CD13 and CD44 expression.
31042409	11	44	theme	cells	1402:1406	arg1	reduction					1375:1383	reduction	1375:1383	reduction of hepatoma stem cells	1375:1406	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	8	45	theme	exogenous	1152:1160	arg1	heparin					1162:1168	exogenous heparin	1152:1168	exogenous heparin	1152:1168	These explained the inhibition of hepatoma sphere formation by exogenous heparin.
31042409	0	46	theme	hepatoma	44:51	arg1	formation					60:68	hepatoma sphere formation	44:68	hepatoma sphere formation	44:68	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	4	47	theme	protein	490:496	arg1	SDC1					510:513	SDC1	510:513	SDC1	510:513	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	47	theme	protein	490:496	arg1	syndecan-1					498:507	the syndecan family protein syndecan-1	470:507	the syndecan family protein syndecan-1 (SDC1)	470:514	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	47	theme	protein	490:496	arg1	markers					425:431	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	5	48	theme	CD44	635:638	arg1	expression					645:654	CD44 gene expression	635:654	CD44 gene expression	635:654	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	5	49	theme	CD44	724:727	arg1	expression					734:743	CD13 and CD44 gene expression	715:743	expression	734:743	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	4	50	theme	syndecan	474:481	arg1	SDC1					510:513	SDC1	510:513	SDC1	510:513	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	50	theme	syndecan	474:481	arg1	syndecan-1					498:507	the syndecan family protein syndecan-1	470:507	the syndecan family protein syndecan-1 (SDC1)	470:514	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	50	theme	syndecan	474:481	arg1	markers					425:431	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	5	51	theme	gene	640:643	arg1	expression					645:654	CD44 gene expression	635:654	CD44 gene expression	635:654	Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression.
31042409	2	52	theme	formation	229:237	arg1	ability					239:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	4	53	theme	CSC	421:423	arg1	markers					425:431	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	53	theme	CSC	421:423	arg1	CD13					433:436	CD13	433:436	CD13	433:436	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	53	theme	CSC	421:423	arg1	CD44					456:459	CD44	456:459	CD44	456:459	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	53	theme	CSC	421:423	arg1	syndecan-1					498:507	the syndecan family protein syndecan-1	470:507	the syndecan family protein syndecan-1 (SDC1)	470:514	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	53	theme	CSC	421:423	arg1	CD90					439:442	CD90	439:442	CD90	439:442	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	53	theme	CSC	421:423	arg1	CD133					445:449	CD133	445:449	CD133	445:449	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	10	54	theme	sphere	1322:1327	arg1	development					1329:1339	hepatoma sphere development	1313:1339	hepatoma sphere development	1313:1339	SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development.
31042409	1	55	theme	many	136:139	arg1	activities					146:155	many cell activities	136:155	many cell activities	136:155	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	1	55	theme	many	136:139	arg1	differentiation					173:187	cell differentiation	168:187	cell differentiation	168:187	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	1	55	theme	many	136:139	arg1	development					203:213	stem cell development	193:213	stem cell development	193:213	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	6	56	theme	formation	891:899	arg1	level					875:879	the level	871:879	the level of sphere formation	871:899	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	7	57	theme	hepatoma	995:1002	arg1	development					1011:1021	hepatoma sphere development	995:1021	hepatoma sphere development	995:1021	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	2	58	theme	sphere	222:227	arg1	ability					239:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	59	theme	stem	278:281	arg1	CSCs					290:293	CSCs	290:293	CSCs	290:293	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	59	theme	stem	278:281	arg1	cells					283:287	cancer stem cells	271:287	cancer stem cells (CSCs)	271:294	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	10	60	theme	hepatoma	1313:1320	arg1	development					1329:1339	hepatoma sphere development	1313:1339	hepatoma sphere development	1313:1339	SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development.
31042409	1	61	theme	cell	141:144	arg1	activities					146:155	many cell activities	136:155	many cell activities	136:155	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	1	61	theme	cell	141:144	arg1	differentiation					173:187	cell differentiation	168:187	cell differentiation	168:187	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	1	61	theme	cell	141:144	arg1	development					203:213	stem cell development	193:213	stem cell development	193:213	Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development.
31042409	11	62	theme	heparin	1357:1363	arg1	Application					1342:1352	Application	1342:1352	Application of heparin mimics in reduction of hepatoma stem cells	1342:1406	Application of heparin mimics in reduction of hepatoma stem cells might be possible.
31042409	7	63	theme	sphere	1004:1009	arg1	development					1011:1021	hepatoma sphere development	995:1021	hepatoma sphere development	995:1021	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	2	64	theme	Tumor	216:220	arg1	ability					239:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability	216:245	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	65	theme	cancer	271:276	arg1	CSCs					290:293	CSCs	290:293	CSCs	290:293	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	65	theme	cancer	271:276	arg1	cells					283:287	cancer stem cells	271:287	cancer stem cells (CSCs)	271:294	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	6	66	theme	sphere	798:803	arg1	development					805:815	sphere development	798:815	sphere development	798:815	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	7	67	theme	chondroitin	956:966	arg1	disaccharide					968:979	chondroitin disaccharide	956:979	chondroitin disaccharide	956:979	The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation.
31042409	9	68	theme	CD44	1234:1237	arg1	expression					1239:1248	CSC marker CD13 and CD44 expression	1214:1248	expression	1239:1248	In conclusion, we found that SDC1 affected CSC marker CD13 and CD44 expression.
31042409	4	69	dep	markers	425:431	arg1	markers					425:431	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1),	421:515	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	CD13					433:436	CD13	433:436	CD13	433:436	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	CD44					456:459	CD44	456:459	CD44	456:459	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	syndecan-1					498:507	the syndecan family protein syndecan-1	470:507	the syndecan family protein syndecan-1 (SDC1)	470:514	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	CD90					439:442	CD90	439:442	CD90	439:442	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	SDC1					510:513	SDC1	510:513	SDC1	510:513	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	4	69	dep	markers	425:431	arg1	CD133					445:449	CD133	445:449	CD133	445:449	Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR.
31042409	0	70	theme	sphere	53:58	arg1	formation					60:68	hepatoma sphere formation	44:68	hepatoma sphere formation	44:68	Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
31042409	6	71	theme	sphere	884:889	arg1	formation					891:899	sphere formation	884:899	sphere formation	884:899	Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation.
31042409	2	72	theme	properties	257:266	arg1	one					250:252	one	250:252	one	250:252	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	2	72	theme	properties	257:266	arg1	properties					257:266	properties	257:266	properties in cancer stem cells (CSCs)	257:294	Tumor sphere formation ability is one of properties in cancer stem cells (CSCs).
31042409	8	73	theme	formation	1139:1147	arg1	inhibition					1109:1118	the inhibition	1105:1118	the inhibition of hepatoma sphere formation by exogenous heparin	1105:1168	These explained the inhibition of hepatoma sphere formation by exogenous heparin.
31677839	8	0	theme	RO	1125:1126	arg1	concentrate					1128:1138	RO concentrate	1125:1138	RO concentrate	1125:1138	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	0	1	from	differences	18:28	arg1	properties					47:56	cheese-making properties	33:56	cheese-making properties	33:56	Understanding the differences in cheese-making properties between reverse osmosis and ultrafiltration concentrates.
31677839	7	2	theme	yield	1026:1030	arg1	increase					989:996	the 7% increase	982:996	the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate	982:1058	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	2	3	theme	mineral	360:366	arg1	contents					368:375	mineral contents	360:375	mineral contents	360:375	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	0	4	theme	ultrafiltration	86:100	arg1	concentrates					102:113	ultrafiltration concentrates	86:113	ultrafiltration concentrates	86:113	Understanding the differences in cheese-making properties between reverse osmosis and ultrafiltration concentrates.
31677839	4	5	theme	rennet	687:692	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	5	theme	rennet	687:692	arg1	behavior					703:710	rennet gelation behavior	687:710	rennet gelation behavior	687:710	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	10	6	theme	mineral	1521:1527	arg1	content					1529:1535	high mineral content	1516:1535	high mineral content in RO concentrate	1516:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	10	7	theme	RO	1540:1541	arg1	concentrate					1543:1553	RO concentrate	1540:1553	RO concentrate	1540:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	3	8	theme	concentrate	529:539	arg1	properties					512:521	the cheese-making properties	494:521	the cheese-making properties of RO concentrate	494:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	10	9	from	concentrate	1543:1553	arg1	effects					1505:1511	the negative effects	1492:1511	the negative effects of high mineral content in RO concentrate	1492:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	7	10	theme	moisture-adjusted	1001:1017	arg1	yield					1026:1030	moisture-adjusted cheese yield	1001:1030	moisture-adjusted cheese yield	1001:1030	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	7	11	theme	RO	942:943	arg1	concentrate					945:955	RO concentrate	942:955	RO concentrate	942:955	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	4	12	theme	gelation	694:701	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	12	theme	gelation	694:701	arg1	behavior					703:710	rennet gelation behavior	687:710	rennet gelation behavior	687:710	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	8	13	theme	moisture	1154:1161	arg1	content					1163:1169	higher moisture content	1147:1169	higher moisture content	1147:1169	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	3	14	theme	high	477:480	arg1	minerals					482:489	high minerals	477:489	high minerals	477:489	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	4	15	theme	concentrates	624:635	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	15	theme	concentrates	624:635	arg1	equilibria					610:619	The soluble colloidal equilibria	588:619	The soluble colloidal equilibria of concentrates	588:635	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	2	16	theme	latter	384:389	arg1	lactose					348:354	the high lactose	339:354	the high lactose	339:354	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	2	16	theme	latter	384:389	arg1	contents					368:375	mineral contents	360:375	mineral contents	360:375	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	2	16	theme	latter	384:389	arg1	differences					274:284	The main compositional differences	251:284	The main compositional differences between ultrafiltration (UF) and RO concentrates	251:333	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	8	17	theme	higher	1147:1152	arg1	content					1163:1169	higher moisture content	1147:1169	higher moisture content	1147:1169	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	1	18	contain	has	159:161	arg2	potential					167:175	the potential to increase cheese yield	163:200	the potential to increase cheese yield	163:200	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	1	18	contain	has	159:161	arg1	milk					130:133	Concentrating milk	116:133	Concentrating milk by reverse osmosis (RO)	116:157	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	3	19	theme	RO	526:527	arg1	concentrate					529:539	RO concentrate	526:539	RO concentrate	526:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	10	20	theme	content	1529:1535	arg1	effects					1505:1511	the negative effects	1492:1511	the negative effects of high mineral content in RO concentrate	1492:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	5	21	theme	Rennet	767:772	arg1	time					786:789	Rennet coagulation time	767:789	Rennet coagulation time	767:789	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	3	22	theme	UF	559:560	arg1	concentrate					562:572	UF concentrate	559:572	UF concentrate	559:572	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	1	23	theme	cheese	189:194	arg1	yield					196:200	cheese yield	189:200	cheese yield	189:200	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	5	24	theme	gel	806:808	arg1	rate					818:821	gel firming rate	806:821	gel firming rate	806:821	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	7	25	theme	UF	1045:1046	arg1	concentrate					1048:1058	UF concentrate	1045:1058	UF concentrate	1045:1058	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	1	26	theme	Concentrating	116:128	arg1	milk					130:133	Concentrating milk	116:133	Concentrating milk by reverse osmosis (RO)	116:157	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	8	27	theme	high	1224:1227	arg1	concentration					1237:1249	the high mineral concentration	1220:1249	the high mineral concentration	1220:1249	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	4	28	theme	several	661:667	arg1	microstructure					751:764	microstructure	751:764	microstructure	751:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	28	theme	several	661:667	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	28	theme	several	661:667	arg1	balance					725:731	cheese mass balance	713:731	cheese mass balance	713:731	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	28	theme	several	661:667	arg1	equilibria					610:619	The soluble colloidal equilibria	588:619	The soluble colloidal equilibria of concentrates	588:635	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	28	theme	several	661:667	arg1	composition					734:744	composition	734:744	composition	734:744	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	28	theme	several	661:667	arg1	behavior					703:710	rennet gelation behavior	687:710	rennet gelation behavior	687:710	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	5	29	theme	firming	810:816	arg1	rate					818:821	gel firming rate	806:821	gel firming rate	806:821	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	10	30	theme	appropriate	1459:1469	arg1	strategies					1471:1480	appropriate strategies	1459:1480	appropriate strategies	1459:1480	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	8	31	theme	mineral	1229:1235	arg1	concentration					1237:1249	the high mineral concentration	1220:1249	the high mineral concentration	1220:1249	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	2	32	theme	RO	319:320	arg1	concentrates					322:333	RO concentrates	319:333	RO concentrates	319:333	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	9	33	theme	UF	1307:1308	arg1	concentrate					1310:1320	UF concentrate	1307:1320	UF concentrate	1307:1320	This study showed the potential of using RO instead of UF concentrate to maximize cheese yield.
31677839	10	34	from	effects	1505:1511	arg1	concentrate					1543:1553	RO concentrate	1540:1553	RO concentrate	1540:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	3	35	theme	high	460:463	arg1	lactose					465:471	high lactose	460:471	high lactose	460:471	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	5	36	theme	coagulation	774:784	arg1	time					786:789	Rennet coagulation time	767:789	Rennet coagulation time	767:789	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	2	37	theme	high	343:346	arg1	lactose					348:354	the high lactose	339:354	the high lactose	339:354	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	2	37	theme	high	343:346	arg1	differences					274:284	The main compositional differences	251:284	The main compositional differences between ultrafiltration (UF) and RO concentrates	251:333	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	7	38	from	Lactose	931:937	arg1	concentrate					945:955	RO concentrate	942:955	RO concentrate	942:955	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	5	39	theme	UF	861:862	arg1	concentrate					864:874	UF concentrate	861:874	UF concentrate	861:874	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	3	40	theme	distinct	440:447	arg1	effects					449:455	the distinct effects	436:455	the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate	436:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	0	41	theme	cheese-making	33:45	arg1	properties					47:56	cheese-making properties	33:56	cheese-making properties	33:56	Understanding the differences in cheese-making properties between reverse osmosis and ultrafiltration concentrates.
31677839	3	42	theme	work	414:417	arg1	objective					396:404	The objective	392:404	The objective of this work	392:417	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	5	43	theme	RO	837:838	arg1	concentrate					840:850	RO concentrate	837:850	RO concentrate	837:850	Rennet coagulation time was longer and gel firming rate was lower for RO concentrate than for UF concentrate.
31677839	0	44	theme	reverse	66:72	arg1	osmosis					74:80	reverse osmosis	66:80	reverse osmosis	66:80	Understanding the differences in cheese-making properties between reverse osmosis and ultrafiltration concentrates.
31677839	4	45	theme	other	669:673	arg1	microstructure					751:764	microstructure	751:764	microstructure	751:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	45	theme	other	669:673	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	45	theme	other	669:673	arg1	balance					725:731	cheese mass balance	713:731	cheese mass balance	713:731	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	45	theme	other	669:673	arg1	equilibria					610:619	The soluble colloidal equilibria	588:619	The soluble colloidal equilibria of concentrates	588:635	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	45	theme	other	669:673	arg1	composition					734:744	composition	734:744	composition	734:744	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	45	theme	other	669:673	arg1	behavior					703:710	rennet gelation behavior	687:710	rennet gelation behavior	687:710	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	8	46	theme	UF	1092:1093	arg1	concentrate					1095:1105	UF concentrate	1092:1105	UF concentrate	1092:1105	Compared with cheese made from UF concentrate, cheese made from RO concentrate showed higher moisture content, which could not be attributed to lactose but to the high mineral concentration.
31677839	3	47	from	effects	449:455	arg1	properties					512:521	the cheese-making properties	494:521	the cheese-making properties of RO concentrate	494:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	1	48	theme	reverse	138:144	arg1	RO					155:156	RO	155:156	RO	155:156	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	1	48	theme	reverse	138:144	arg1	osmosis					146:152	reverse osmosis	138:152	reverse osmosis (RO)	138:157	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	4	49	theme	soluble	592:598	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	49	theme	soluble	592:598	arg1	equilibria					610:619	The soluble colloidal equilibria	588:619	The soluble colloidal equilibria of concentrates	588:635	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	9	50	theme	cheese	1334:1339	arg1	yield					1341:1345	cheese yield	1334:1345	cheese yield	1334:1345	This study showed the potential of using RO instead of UF concentrate to maximize cheese yield.
31677839	7	51	theme	relative	1033:1040	arg1	yield					1026:1030	moisture-adjusted cheese yield	1001:1030	moisture-adjusted cheese yield	1001:1030	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	10	52	from	content	1529:1535	arg1	concentrate					1543:1553	RO concentrate	1540:1553	RO concentrate	1540:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	7	53	theme	%	987:987	arg1	increase					989:996	the 7% increase	982:996	the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate	982:1058	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	3	54	theme	minerals	482:489	arg1	effects					449:455	the distinct effects	436:455	the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate	436:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	4	55	theme	mass	720:723	arg1	balance					725:731	cheese mass balance	713:731	cheese mass balance	713:731	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	55	theme	mass	720:723	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	7	56	theme	cheese	1019:1024	arg1	yield					1026:1030	moisture-adjusted cheese yield	1001:1030	moisture-adjusted cheese yield	1001:1030	Lactose in RO concentrate was also responsible for the 7% increase of moisture-adjusted cheese yield, relative to UF concentrate.
31677839	9	57	theme	concentrate	1310:1320	arg1	RO					1293:1294	RO	1293:1294	RO instead of UF concentrate	1293:1320	This study showed the potential of using RO instead of UF concentrate to maximize cheese yield.
31677839	3	58	theme	lactose	465:471	arg1	effects					449:455	the distinct effects	436:455	the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate	436:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	4	59	theme	colloidal	600:608	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	59	theme	colloidal	600:608	arg1	equilibria					610:619	The soluble colloidal equilibria	588:619	The soluble colloidal equilibria of concentrates	588:635	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	60	dep	properties	675:684	arg1	composition					734:744	composition	734:744	composition	734:744	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	60	dep	properties	675:684	arg1	microstructure					751:764	microstructure	751:764	microstructure	751:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	60	dep	properties	675:684	arg1	balance					725:731	cheese mass balance	713:731	cheese mass balance	713:731	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	60	dep	properties	675:684	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	60	dep	properties	675:684	arg1	behavior					703:710	rennet gelation behavior	687:710	rennet gelation behavior	687:710	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	2	61	theme	compositional	260:272	arg1	lactose					348:354	the high lactose	339:354	the high lactose	339:354	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	2	61	theme	compositional	260:272	arg1	differences					274:284	The main compositional differences	251:284	The main compositional differences between ultrafiltration (UF) and RO concentrates	251:333	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	4	62	theme	cheese	713:718	arg1	balance					725:731	cheese mass balance	713:731	cheese mass balance	713:731	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	4	62	theme	cheese	713:718	arg1	properties					675:684	several other properties	661:684	several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure	661:764	The soluble colloidal equilibria of concentrates were studied as well as several other properties: rennet gelation behavior, cheese mass balance, composition, and microstructure.
31677839	1	63	theme	cheese-making	225:237	arg1	properties					239:248	cheese-making properties	225:248	cheese-making properties	225:248	Concentrating milk by reverse osmosis (RO) has the potential to increase cheese yield but is known to impair cheese-making properties.
31677839	10	64	theme	high	1516:1519	arg1	content					1529:1535	high mineral content	1516:1535	high mineral content in RO concentrate	1516:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
31677839	2	65	theme	main	255:258	arg1	lactose					348:354	the high lactose	339:354	the high lactose	339:354	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	2	65	theme	main	255:258	arg1	differences					274:284	The main compositional differences	251:284	The main compositional differences between ultrafiltration (UF) and RO concentrates	251:333	The main compositional differences between ultrafiltration (UF) and RO concentrates are the high lactose and mineral contents of the latter.
31677839	3	66	theme	cheese-making	498:510	arg1	properties					512:521	the cheese-making properties	494:521	the cheese-making properties of RO concentrate	494:539	The objective of this work was to determine the distinct effects of high lactose and high minerals on the cheese-making properties of RO concentrate, by supplementing UF concentrate with lactose.
31677839	10	67	theme	negative	1496:1503	arg1	effects					1505:1511	the negative effects	1492:1511	the negative effects of high mineral content in RO concentrate	1492:1553	The approach is, however, limited to applications where post-acidification can be controlled, and will require appropriate strategies to reduce the negative effects of high mineral content in RO concentrate.
30221832	1	0	theme	herbivore	150:158	arg1	colonizer					137:145	a late colonizer	130:145	a late colonizer of herbivore dung	130:163	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	1	0	theme	herbivore	150:158	arg1	machinery					210:218	an enzymatic machinery	197:218	an enzymatic machinery	197:218	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	1	1	theme	recalcitrant	240:251	arg1	fraction					253:260	the more recalcitrant fraction	231:260	the more recalcitrant fraction of plant biomass	231:277	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	6	2	from	view	863:866	arg1	adaptation					890:899	the transcriptomic adaptation	871:899	the transcriptomic adaptation of P. anserina to substrate composition	871:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	6	3	theme	depth	857:861	arg1	view					863:866	an in depth view	851:866	an in depth view on the transcriptomic adaptation of P. anserina to substrate composition	851:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	1	4	theme	dung	160:163	arg1	herbivore					150:158	herbivore dung	150:163	herbivore dung	150:163	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	0	5	theme	Commercial	98:107	arg1	Cocktails					116:124	Optimized Commercial Enzyme Cocktails	88:124	Optimized Commercial Enzyme Cocktails	88:124	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	6	6	theme	in	854:855	arg1	view					863:866	an in depth view	851:866	an in depth view on the transcriptomic adaptation of P. anserina to substrate composition	851:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	0	7	theme	Optimized	88:96	arg1	Cocktails					116:124	Optimized Commercial Enzyme Cocktails	88:124	Optimized Commercial Enzyme Cocktails	88:124	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	1	8	theme	plant	265:269	arg1	biomass					271:277	plant biomass	265:277	plant biomass	265:277	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	5	9	theme	previous	793:800	arg1	studies					813:819	previous proteomics studies	793:819	previous proteomics studies	793:819	Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies.
30221832	6	10	theme	industrial	1024:1033	arg1	level					1035:1039	the industrial level	1020:1039	the industrial level	1020:1039	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	2	11	theme	industrial	405:414	arg1	stover					457:462	corn stover	452:462	corn stover (CS)	452:467	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	11	theme	industrial	405:414	arg1	feedstocks					416:425	two industrial feedstocks	401:425	two industrial feedstocks	401:425	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	11	theme	industrial	405:414	arg1	hulls					436:440	soybean hulls	428:440	soybean hulls (SBH)	428:446	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	0	12	theme	Enzyme	109:114	arg1	Cocktails					116:124	Optimized Commercial Enzyme Cocktails	88:124	Optimized Commercial Enzyme Cocktails	88:124	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	1	13	theme	Podospora	166:174	arg1	anserina					176:183	Podospora anserina	166:183	Podospora anserina	166:183	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	6	14	theme	substrate	919:927	arg1	composition					929:939	substrate composition	919:939	substrate composition	919:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	3	15	theme	amylolytic	542:551	arg1	genes					553:557	hemicellulolytic and amylolytic genes	521:557	hemicellulolytic and amylolytic genes	521:557	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	0	16	theme	Enzymatic	0:8	arg1	Adaptation					10:19	Enzymatic Adaptation	0:19	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass	0:68	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	1	17	theme	biomass	271:277	arg1	fraction					253:260	the more recalcitrant fraction	231:260	the more recalcitrant fraction of plant biomass	231:277	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	3	18	theme	genes	553:557	arg1	expression					507:516	the expression	503:516	the expression of hemicellulolytic and amylolytic genes, respectively	503:571	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	6	19	theme	biomass	1009:1015	arg1	saccharification					983:998	saccharification	983:998	saccharification of plant biomass at the industrial level	983:1039	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	0	20	theme	Podospora	24:32	arg1	anserina					34:41	Podospora anserina	24:41	Podospora anserina	24:41	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	2	21	from	transcriptome	370:382	arg1	stover					457:462	corn stover	452:462	corn stover (CS)	452:467	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	21	from	transcriptome	370:382	arg1	feedstocks					416:425	two industrial feedstocks	401:425	two industrial feedstocks	401:425	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	21	from	transcriptome	370:382	arg1	hulls					436:440	soybean hulls	428:440	soybean hulls (SBH)	428:446	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	0	22	dep	Provides	70:77	arg1	Leads					79:83	Leads	79:83	Provides Leads to Optimized Commercial Enzyme Cocktails	70:124	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	6	23	theme	plant	1003:1007	arg1	biomass					1009:1015	plant biomass	1003:1015	plant biomass	1003:1015	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	5	24	theme	genes	775:779	arg1	diversity					752:760	a larger diversity	743:760	a larger diversity of expressed genes	743:779	Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies.
30221832	6	25	theme	anserina	907:914	arg1	adaptation					890:899	the transcriptomic adaptation	871:899	the transcriptomic adaptation of P. anserina to substrate composition	871:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	3	26	theme	diverse	608:614	arg1	set					621:623	a more diverse gene set	601:623	a more diverse gene set	601:623	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	4	27	theme	carbon	699:704	arg1	catabolism					706:715	carbon catabolism	699:715	carbon catabolism	699:715	Substrate adaptation is also observed for carbon catabolism.
30221832	2	28	theme	soybean	428:434	arg1	SBH					443:445	SBH	443:445	SBH	443:445	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	28	theme	soybean	428:434	arg1	feedstocks					416:425	two industrial feedstocks	401:425	two industrial feedstocks	401:425	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	28	theme	soybean	428:434	arg1	hulls					436:440	soybean hulls	428:440	soybean hulls (SBH)	428:446	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	6	29	theme	P.	904:905	arg1	anserina					907:914	P. anserina	904:914	P. anserina	904:914	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	3	30	theme	gene	616:619	arg1	set					621:623	a more diverse gene set	601:623	a more diverse gene set	601:623	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	1	31	theme	enzymatic	200:208	arg1	colonizer					137:145	a late colonizer	130:145	a late colonizer of herbivore dung	130:163	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	1	31	theme	enzymatic	200:208	arg1	machinery					210:218	an enzymatic machinery	197:218	an enzymatic machinery	197:218	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	0	32	theme	anserina	34:41	arg1	Adaptation					10:19	Enzymatic Adaptation	0:19	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass	0:68	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	1	33	theme	great	293:297	arg1	potential					299:307	a great potential	291:307	a great potential for biotechnology applications	291:338	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	5	34	theme	expressed	765:773	arg1	genes					775:779	expressed genes	765:779	expressed genes	765:779	Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies.
30221832	5	35	theme	proteomics	802:811	arg1	studies					813:819	previous proteomics studies	793:819	previous proteomics studies	793:819	Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies.
30221832	0	36	theme	Plant	56:60	arg1	Biomass					62:68	Different Plant Biomass	46:68	Different Plant Biomass	46:68	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	5	37	theme	larger	745:750	arg1	diversity					752:760	a larger diversity	743:760	a larger diversity of expressed genes	743:779	Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies.
30221832	0	38	theme	Different	46:54	arg1	Biomass					62:68	Different Plant Biomass	46:68	Different Plant Biomass	46:68	Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails.
30221832	6	39	theme	transcriptomic	875:888	arg1	adaptation					890:899	the transcriptomic adaptation	871:899	the transcriptomic adaptation of P. anserina to substrate composition	871:939	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	2	40	theme	corn	452:455	arg1	stover					457:462	corn stover	452:462	corn stover (CS)	452:467	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	40	theme	corn	452:455	arg1	feedstocks					416:425	two industrial feedstocks	401:425	two industrial feedstocks	401:425	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	2	40	theme	corn	452:455	arg1	CS					465:466	CS	465:466	CS	465:466	The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS).
30221832	4	41	theme	Substrate	657:665	arg1	adaptation					667:676	Substrate adaptation	657:676	Substrate adaptation	657:676	Substrate adaptation is also observed for carbon catabolism.
30221832	1	42	theme	biotechnology	313:325	arg1	applications					327:338	biotechnology applications	313:338	biotechnology applications	313:338	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	3	43	theme	time	589:592	arg1	points					594:599	later time points	583:599	later time points	583:599	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	3	44	theme	hemicellulolytic	521:536	arg1	genes					553:557	hemicellulolytic and amylolytic genes	521:557	hemicellulolytic and amylolytic genes	521:557	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
30221832	1	45	theme	late	132:135	arg1	colonizer					137:145	a late colonizer	130:145	a late colonizer of herbivore dung	130:163	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	1	45	theme	late	132:135	arg1	machinery					210:218	an enzymatic machinery	197:218	an enzymatic machinery	197:218	As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications.
30221832	6	46	from	level	1035:1039	arg1	saccharification					983:998	saccharification	983:998	saccharification of plant biomass at the industrial level	983:1039	The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
30221832	3	47	theme	later	583:587	arg1	points					594:599	later time points	583:599	later time points	583:599	Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH.
31255619	0	0	theme	agarase	95:101	arg1	hydrolysis					103:112	agarase hydrolysis	95:112	agarase hydrolysis	95:112	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	3	1	theme	polymerization	506:519	arg1	degrees					495:501	different degrees	485:501	different degrees of polymerization (DP)	485:524	In this study, Gracilaria crude polysaccharide was treated with agarase and hydrolyzed into NAOSs with different degrees of polymerization (DP).
31255619	9	2	from	effect	1095:1100	arg1	juice					1152:1156	simulated gastrointestinal juice	1125:1156	simulated gastrointestinal juice	1125:1156	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	11	3	theme	considerable	1431:1442	arg1	potential					1444:1452	a considerable potential	1429:1452	a considerable potential	1429:1452	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	5	4	theme	antioxidant	677:687	arg1	capacity					689:696	antioxidant capacity	677:696	antioxidant capacity	677:696	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	11	5	theme	Gracilaria	1347:1356	arg1	prebiotic					1399:1407	prebiotic	1399:1407	prebiotic	1399:1407	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	5	theme	Gracilaria	1347:1356	arg1	antioxidant					1383:1393	antioxidant	1383:1393	antioxidant	1383:1393	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	5	theme	Gracilaria	1347:1356	arg1	NAOSs					1358:1362	Gracilaria NAOSs	1347:1362	Gracilaria NAOSs	1347:1362	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	6	contain	had	1425:1427	arg1	prebiotic					1399:1407	prebiotic	1399:1407	prebiotic	1399:1407	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	6	contain	had	1425:1427	arg1	antioxidant					1383:1393	antioxidant	1383:1393	antioxidant	1383:1393	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	6	contain	had	1425:1427	arg1	NAOSs					1358:1362	Gracilaria NAOSs	1347:1362	Gracilaria NAOSs	1347:1362	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	6	contain	had	1425:1427	arg2	potential					1444:1452	a considerable potential	1429:1452	a considerable potential	1429:1452	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	10	7	from	effect	1287:1292	arg1	growth					1309:1314	Procambarus growth	1297:1314	Procambarus growth	1297:1314	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	0	8	dep	oligosaccharides	66:81	arg1	prepared					83:90	prepared	83:90	oligosaccharides prepared by agarase hydrolysis	66:112	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	5	9	with	capacity	689:696	arg1	DPs					744:746	different DPs	734:746	different DPs	734:746	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	9	10	theme	NAOSs	1116:1120	arg1	effect					1095:1100	The protective effect	1080:1100	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice	1080:1156	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	1	11	theme	red	140:142	arg1	algae					144:148	red algae	140:148	red algae widely cultivated in Asia	140:174	Gracilaria is a genus of red algae widely cultivated in Asia and is notable for its economic importance as food ingredients.
31255619	10	12	used	used	1224:1227	arg2	NAOSs					1185:1189	NAOSs	1185:1189	NAOSs with best prebiotic effects	1185:1217	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	3	13	theme	Gracilaria	397:406	arg1	polysaccharide					414:427	Gracilaria crude polysaccharide	397:427	Gracilaria crude polysaccharide	397:427	In this study, Gracilaria crude polysaccharide was treated with agarase and hydrolyzed into NAOSs with different degrees of polymerization (DP).
31255619	5	14	theme	NAOSs	838:842	arg1	capacity					826:833	the antioxidant capacity	810:833	the antioxidant capacity of NAOSs	810:842	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	9	15	theme	simulated	1125:1133	arg1	juice					1152:1156	simulated gastrointestinal juice	1125:1156	simulated gastrointestinal juice	1125:1156	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	11	16	used	utilized	1371:1378	arg2	prebiotic					1399:1407	prebiotic	1399:1407	prebiotic	1399:1407	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	16	used	utilized	1371:1378	arg2	NAOSs					1358:1362	Gracilaria NAOSs	1347:1362	Gracilaria NAOSs	1347:1362	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	11	16	used	utilized	1371:1378	arg2	antioxidant					1383:1393	antioxidant	1383:1393	antioxidant	1383:1393	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	5	17	dep	capacity	689:696	arg1	The					673:675	The	673:675	The	673:675	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	11	18	dep	antioxidant	1383:1393	arg1	additive					1409:1416	additive	1409:1416	additive	1409:1416	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	2	19	theme	excellent	311:319	arg1	solubility					327:336	excellent water solubility	311:336	excellent water solubility	311:336	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	9	20	theme	gastrointestinal	1135:1150	arg1	juice					1152:1156	simulated gastrointestinal juice	1125:1156	simulated gastrointestinal juice	1125:1156	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	0	21	theme	Antioxidant	0:10	arg1	capacity					12:19	Antioxidant capacity	0:19	Antioxidant capacity	0:19	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	4	22	theme	hydrolyzed	551:560	arg1	NAOSs					562:566	the hydrolyzed NAOSs	547:566	the hydrolyzed NAOSs	547:566	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	5	23	with	effects	712:718	arg1	DPs					744:746	different DPs	734:746	different DPs	734:746	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	0	24	theme	prebiotic	25:33	arg1	effects					35:41	prebiotic effects	25:41	prebiotic effects	25:41	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	10	25	theme	prebiotic	1201:1209	arg1	effects					1211:1217	best prebiotic effects	1196:1217	best prebiotic effects	1196:1217	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	4	26	theme	layer	651:655	arg1	chromatography					657:670	thin layer chromatography	646:670	thin layer chromatography	646:670	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	7	27	theme	delbrueckii	1018:1028	arg1	subsp					1030:1034	Lactobacillus delbrueckii subsp	1004:1034	Lactobacillus delbrueckii subsp	1004:1034	NAOSs promoted the growth of Lactobacillus delbrueckii subsp.
31255619	10	28	theme	feeding	1244:1250	arg1	experiment					1252:1261	Procambarus feeding experiment	1232:1261	Procambarus feeding experiment	1232:1261	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	5	29	theme	different	734:742	arg1	DPs					744:746	different DPs	734:746	different DPs	734:746	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	11	30	dep	food	1457:1460	arg1	industry					1471:1478	industry	1471:1478	industry	1471:1478	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	2	31	theme	Gracilaria	290:299	arg1	products					278:285	products	278:285	products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect	278:379	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	31	theme	Gracilaria	290:299	arg1	oligosaccharides					249:264	Neoagaro oligosaccharides	240:264	Neoagaro oligosaccharides (NAOSs)	240:272	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	4	32	theme	thin	646:649	arg1	chromatography					657:670	thin layer chromatography	646:670	thin layer chromatography	646:670	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	3	33	theme	crude	408:412	arg1	polysaccharide					414:427	Gracilaria crude polysaccharide	397:427	Gracilaria crude polysaccharide	397:427	In this study, Gracilaria crude polysaccharide was treated with agarase and hydrolyzed into NAOSs with different degrees of polymerization (DP).
31255619	4	34	theme	NAOSs	562:566	arg1	compositions					531:542	The compositions	527:542	The compositions of the hydrolyzed NAOSs	527:566	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	10	35	theme	promotion	1277:1285	arg1	effect					1287:1292	promotion effect	1277:1292	promotion effect on Procambarus growth	1277:1314	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	1	36	theme	economic	199:206	arg1	importance					208:217	its economic importance	195:217	its economic importance as food ingredients	195:237	Gracilaria is a genus of red algae widely cultivated in Asia and is notable for its economic importance as food ingredients.
31255619	6	37	theme	bacteria	965:972	arg1	growth					939:944	the growth	935:944	the growth of four intestinal bacteria	935:972	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	3	38	with	NAOSs	474:478	arg1	degrees					495:501	different degrees	485:501	different degrees of polymerization (DP)	485:524	In this study, Gracilaria crude polysaccharide was treated with agarase and hydrolyzed into NAOSs with different degrees of polymerization (DP).
31255619	6	39	theme	different	881:889	arg1	DP					891:892	different DP	881:892	different DP	881:892	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	4	40	theme	chromatography	657:670	arg1	ionization-time					598:612	electrospray ionization-time	585:612	electrospray ionization-time of flight-mass spectrometry and thin layer chromatography	585:670	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	0	41	theme	neoagaro	57:64	arg1	capacity					12:19	Antioxidant capacity	0:19	Antioxidant capacity	0:19	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	0	41	theme	neoagaro	57:64	arg1	effects					35:41	prebiotic effects	25:41	prebiotic effects	25:41	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	6	42	theme	intestinal	954:963	arg1	bacteria					965:972	four intestinal bacteria	949:972	four intestinal bacteria	949:972	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	5	43	theme	prebiotic	702:710	arg1	effects					712:718	prebiotic effects	702:718	prebiotic effects	702:718	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	6	44	from	influence	922:930	arg1	growth					939:944	the growth	935:944	the growth of four intestinal bacteria	935:972	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	10	45	with	NAOSs	1185:1189	arg1	effects					1211:1217	best prebiotic effects	1196:1217	best prebiotic effects	1196:1217	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	2	46	theme	antioxidant	339:349	arg1	activity					351:358	antioxidant activity	339:358	antioxidant activity	339:358	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	0	47	theme	Gracilaria	46:55	arg1	neoagaro					57:64	Gracilaria neoagaro	46:64	Gracilaria neoagaro	46:64	Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis.
31255619	2	48	contain	have	306:309	arg1	products					278:285	products	278:285	products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect	278:379	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	48	contain	have	306:309	arg2	effect					374:379	prebiotic effect	364:379	prebiotic effect	364:379	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	48	contain	have	306:309	arg2	activity					351:358	antioxidant activity	339:358	antioxidant activity	339:358	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	48	contain	have	306:309	arg2	solubility					327:336	excellent water solubility	311:336	excellent water solubility	311:336	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	48	contain	have	306:309	arg1	oligosaccharides					249:264	Neoagaro oligosaccharides	240:264	Neoagaro oligosaccharides (NAOSs)	240:272	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	11	49	theme	present	1321:1327	arg1	study					1329:1333	The present study	1317:1333	The present study	1317:1333	The present study showed that Gracilaria NAOSs can be utilized as antioxidant and prebiotic additive, which had a considerable potential in food and feed industry.
31255619	6	50	theme	NAOSs	870:874	arg1	effects					859:865	The prebiotic effects	845:865	The prebiotic effects of NAOSs with different DP	845:892	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	7	51	theme	subsp	1030:1034	arg1	growth					994:999	the growth	990:999	the growth of Lactobacillus delbrueckii subsp	990:1034	NAOSs promoted the growth of Lactobacillus delbrueckii subsp.
31255619	10	52	theme	Procambarus	1297:1307	arg1	growth					1309:1314	Procambarus growth	1297:1314	Procambarus growth	1297:1314	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	9	53	theme	Gracilaria	1105:1114	arg1	NAOSs					1116:1120	Gracilaria NAOSs	1105:1120	Gracilaria NAOSs	1105:1120	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	4	54	theme	flight-mass	617:627	arg1	spectrometry					629:640	flight-mass spectrometry	617:640	flight-mass spectrometry	617:640	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	5	55	theme	NAOSs	723:727	arg1	capacity					689:696	antioxidant capacity	677:696	antioxidant capacity	677:696	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	5	55	theme	NAOSs	723:727	arg1	effects					712:718	prebiotic effects	702:718	prebiotic effects	702:718	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	7	56	theme	Lactobacillus	1004:1016	arg1	subsp					1030:1034	Lactobacillus delbrueckii subsp	1004:1034	Lactobacillus delbrueckii subsp	1004:1034	NAOSs promoted the growth of Lactobacillus delbrueckii subsp.
31255619	2	57	theme	water	321:325	arg1	solubility					327:336	excellent water solubility	311:336	excellent water solubility	311:336	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	1	58	theme	food	222:225	arg1	ingredients					227:237	food ingredients	222:237	food ingredients	222:237	Gracilaria is a genus of red algae widely cultivated in Asia and is notable for its economic importance as food ingredients.
31255619	10	59	theme	Procambarus	1232:1242	arg1	experiment					1252:1261	Procambarus feeding experiment	1232:1261	Procambarus feeding experiment	1232:1261	Finally, NAOSs with best prebiotic effects were used in Procambarus feeding experiment and exhibited promotion effect on Procambarus growth.
31255619	6	60	theme	prebiotic	849:857	arg1	effects					859:865	The prebiotic effects	845:865	The prebiotic effects of NAOSs with different DP	845:892	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	6	61	with	effects	859:865	arg1	DP					891:892	different DP	881:892	different DP	881:892	The prebiotic effects of NAOSs with different DP were evaluated based on the influence on the growth of four intestinal bacteria.
31255619	1	62	theme	algae	144:148	arg1	Gracilaria					115:124	Gracilaria	115:124	Gracilaria	115:124	Gracilaria is a genus of red algae widely cultivated in Asia and is notable for its economic importance as food ingredients.
31255619	1	62	theme	algae	144:148	arg1	genus					131:135	a genus	129:135	a genus of red algae widely cultivated in Asia	129:174	Gracilaria is a genus of red algae widely cultivated in Asia and is notable for its economic importance as food ingredients.
31255619	3	63	theme	different	485:493	arg1	degrees					495:501	different degrees	485:501	different degrees of polymerization (DP)	485:524	In this study, Gracilaria crude polysaccharide was treated with agarase and hydrolyzed into NAOSs with different degrees of polymerization (DP).
31255619	4	64	theme	electrospray	585:596	arg1	ionization-time					598:612	electrospray ionization-time	585:612	electrospray ionization-time of flight-mass spectrometry and thin layer chromatography	585:670	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31255619	5	65	theme	antioxidant	814:824	arg1	capacity					826:833	the antioxidant capacity	810:833	the antioxidant capacity of NAOSs	810:842	The antioxidant capacity and prebiotic effects of NAOSs with different DPs were investigated and the results showed that DP could affect the antioxidant capacity of NAOSs.
31255619	2	66	theme	Neoagaro	240:247	arg1	products					278:285	products	278:285	products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect	278:379	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	66	theme	Neoagaro	240:247	arg1	oligosaccharides					249:264	Neoagaro oligosaccharides	240:264	Neoagaro oligosaccharides (NAOSs)	240:272	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	66	theme	Neoagaro	240:247	arg1	NAOSs					267:271	NAOSs	267:271	NAOSs	267:271	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	2	67	theme	prebiotic	364:372	arg1	effect					374:379	prebiotic effect	364:379	prebiotic effect	364:379	Neoagaro oligosaccharides (NAOSs) are products of Gracilaria that have excellent water solubility, antioxidant activity and prebiotic effect.
31255619	9	68	theme	protective	1084:1093	arg1	effect					1095:1100	The protective effect	1080:1100	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice	1080:1156	The protective effect of Gracilaria NAOSs in simulated gastrointestinal juice was also studied.
31255619	4	69	theme	spectrometry	629:640	arg1	ionization-time					598:612	electrospray ionization-time	585:612	electrospray ionization-time of flight-mass spectrometry and thin layer chromatography	585:670	The compositions of the hydrolyzed NAOSs were analyzed by electrospray ionization-time of flight-mass spectrometry and thin layer chromatography.
31548320	7	0	theme	acid	1522:1525	arg1	composition					1527:1537	fatty acid composition	1516:1537	fatty acid composition	1516:1537	The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition.
31548320	3	1	theme	pseudomallei	534:545	arg1	structure					518:526	The LPS structure	510:526	The LPS structure of B. pseudomallei, the causative agent of melioidosis,	510:582	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	6	2	theme	penta-acylated	1302:1315	arg1	species					1317:1323	predominantly penta-acylated species	1288:1323	predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups	1288:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	3	3	theme	melioidosis	571:581	arg1	pseudomallei					534:545	B. pseudomallei	531:545	B. pseudomallei	531:545	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	3	3	theme	melioidosis	571:581	arg1	agent					562:566	the causative agent	548:566	the causative agent of melioidosis	548:581	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	5	4	theme	Burkholderia	1199:1210	arg1	species					1212:1218	these Burkholderia species	1193:1218	these Burkholderia species	1193:1218	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	6	5	theme	lipid	1271:1275	arg1	A					1277:1277	Burkholderia lipid A	1258:1277	Burkholderia lipid A	1258:1277	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	1	6	theme	Gram-negative	200:212	arg1	bacteria					214:221	Gram-negative bacteria	200:221	Gram-negative bacteria	200:221	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	2	7	attach	isolated	322:329	arg2	LPSs					317:320	LPSs	317:320	LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis,	317:477	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	7	attach	isolated	322:329	arg1	species					351:357	the pathogenic species Burkholderia pseudomallei and Burkholderia mallei	336:407	the pathogenic species Burkholderia pseudomallei and Burkholderia mallei	336:407	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	7	attach	isolated	322:329	arg1	thailandensis					464:476	Burkholderia thailandensis	451:476	Burkholderia thailandensis	451:476	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	7	attach	isolated	322:329	arg1	species					434:440	less-pathogenic species	418:440	less-pathogenic species	418:440	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	7	attach	isolated	322:329	arg2	stimulators					497:507	potent immune stimulators	483:507	potent immune stimulators	483:507	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	7	8	theme	mass	1454:1457	arg1	differences					1459:1469	mass differences	1454:1469	mass differences at m/z 1,511, 1,642, 1,773, and 1,926	1454:1507	The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition.
31548320	3	9	theme	immune	747:752	arg1	recognition					754:764	their immune recognition	741:764	their immune recognition	741:764	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	8	10	theme	Burkholderia	1766:1777	arg1	species					1779:1785	other Burkholderia species	1760:1785	other Burkholderia species	1760:1785	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	6	11	theme	Burkholderia	1258:1269	arg1	A					1277:1277	Burkholderia lipid A	1258:1277	Burkholderia lipid A	1258:1277	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	0	12	theme	Melioidosis	144:154	arg1	Sera					164:167	Melioidosis Patient Sera	144:167	Melioidosis Patient Sera	144:167	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	1	13	theme	A	238:238	arg1	components					274:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	3	14	attach	isolated	649:656	arg2	LPSs					644:647	the LPSs	640:647	the LPSs isolated from other, related species	640:684	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	3	14	attach	isolated	649:656	arg1	species					678:684	other, related species	663:684	other, related species	663:684	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	6	15	theme	MALDI-TOF	1221:1229	arg1	profiles					1231:1238	MALDI-TOF profiles	1221:1238	MALDI-TOF profiles	1221:1238	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	6	16	theme	4-amino-4-deoxy-arabinose	1339:1363	arg1	residues					1365:1372	4-amino-4-deoxy-arabinose residues	1339:1372	4-amino-4-deoxy-arabinose residues at both terminal phosphate groups	1339:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	5	17	theme	chromatography	1079:1092	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	10	18	contain	have	2030:2033	arg2	implications					2035:2046	implications	2035:2046	implications for serological diagnostics	2035:2074	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	10	18	contain	have	2030:2033	arg1	they					2025:2028	they	2025:2028	they	2025:2028	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	0	19	theme	A	77:77	arg1	Structures					79:88	Different Lipid A Structures	61:88	Different Lipid A Structures	61:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	10	20	theme	antibodies	2102:2111	arg1	detection					2089:2097	the detection	2085:2097	the detection of antibodies to OPS	2085:2118	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	9	21	theme	immunoblot	1803:1812	arg1	analysis					1814:1821	immunoblot analysis	1803:1821	immunoblot analysis	1803:1821	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	0	22	theme	Patient	156:162	arg1	Sera					164:167	Melioidosis Patient Sera	144:167	Melioidosis Patient Sera	144:167	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	0	23	theme	Receptor	107:114	arg1	Activation					118:127	Toll-Like Receptor 4 Activation	97:127	Toll-Like Receptor 4 Activation	97:127	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	5	24	theme	laser	998:1002	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	2	25	theme	less-pathogenic	418:432	arg1	thailandensis					464:476	Burkholderia thailandensis	451:476	Burkholderia thailandensis	451:476	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	25	theme	less-pathogenic	418:432	arg1	species					434:440	less-pathogenic species	418:440	less-pathogenic species	418:440	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	5	26	theme	receptor	1120:1127	arg1	stimulation					1138:1148	Toll-like receptor 4 (TLR4) stimulation	1110:1148	Toll-like receptor 4 (TLR4) stimulation	1110:1148	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	9	27	theme	melioidosis	1841:1851	arg1	sera					1861:1864	melioidosis patient sera	1841:1864	melioidosis patient sera	1841:1864	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	0	28	from	Species	48:54	arg1	Lipopolysaccharides					0:18	Lipopolysaccharides	0:18	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures	0:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	8	29	theme	NF-κB	1583:1587	arg1	responses					1589:1597	TLR4-dependent NF-κB responses	1568:1597	TLR4-dependent NF-κB responses	1568:1597	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	2	30	dep	species	351:357	arg1	pseudomallei					372:383	Burkholderia pseudomallei	359:383	Burkholderia pseudomallei	359:383	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	30	dep	species	351:357	arg1	mallei					402:407	Burkholderia mallei	389:407	Burkholderia mallei	389:407	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	0	31	theme	Different	25:33	arg1	Species					48:54	Different Burkholderia Species	25:54	Different Burkholderia Species with Different Lipid A Structures	25:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	6	32	contain	contains	1279:1286	arg2	species					1317:1323	predominantly penta-acylated species	1288:1323	predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups	1288:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	6	32	contain	contains	1279:1286	arg1	A					1277:1277	Burkholderia lipid A	1258:1277	Burkholderia lipid A	1258:1277	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	3	33	theme	other	663:667	arg1	species					678:684	other, related species	663:684	other, related species	663:684	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	3	34	from	Thailand	621:628	arg1	isolates					607:614	isolates	607:614	isolates from Thailand	607:628	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	5	35	theme	MALDI-TOF	1060:1068	arg1	spectrometry					1046:1057	flight mass spectrometry	1034:1057	flight mass spectrometry (MALDI-TOF MS)	1034:1072	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	35	theme	MALDI-TOF	1060:1068	arg1	MS					1070:1071	MALDI-TOF MS	1060:1071	MALDI-TOF MS	1060:1071	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	2	36	theme	immune	490:495	arg1	LPSs					317:320	LPSs	317:320	LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis,	317:477	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	36	theme	immune	490:495	arg1	stimulators					497:507	potent immune stimulators	483:507	potent immune stimulators	483:507	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	0	37	theme	Different	61:69	arg1	Structures					79:88	Different Lipid A Structures	61:88	Different Lipid A Structures	61:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	5	38	theme	immunoblot	1155:1164	arg1	analysis					1166:1173	immunoblot analysis	1155:1173	immunoblot analysis	1155:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	9	39	theme	Burkholderia	1899:1910	arg1	species					1912:1918	other Burkholderia species	1893:1918	other Burkholderia species	1893:1918	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	8	40	theme	SDS-PAGE	1615:1622	arg1	analysis					1624:1631	SDS-PAGE analysis	1615:1631	SDS-PAGE analysis	1615:1631	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	5	41	theme	flight	1034:1039	arg1	spectrometry					1046:1057	flight mass spectrometry	1034:1057	flight mass spectrometry (MALDI-TOF MS)	1034:1072	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	41	theme	flight	1034:1039	arg1	MS					1070:1071	MALDI-TOF MS	1060:1071	MALDI-TOF MS	1060:1071	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	7	42	theme	fatty	1516:1520	arg1	composition					1527:1537	fatty acid composition	1516:1537	fatty acid composition	1516:1537	The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition.
31548320	5	43	theme	spectrometry	1046:1057	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	8	44	theme	LPS	1648:1650	arg1	patterns					1659:1666	similar LPS ladder patterns	1640:1666	similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei	1640:1719	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	0	45	with	Species	48:54	arg1	Structures					79:88	Different Lipid A Structures	61:88	Different Lipid A Structures	61:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	1	46	theme	O-polysaccharide	251:266	arg1	components					274:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	4	47	theme	Burkholderia	885:896	arg1	species					898:904	eight Burkholderia species	879:904	eight Burkholderia species	879:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	10	48	theme	LPS	1992:1994	arg1	characteristics					1973:1987	the characteristics	1969:1987	the characteristics of LPS in Burkholderia species	1969:2018	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	3	49	theme	causative	552:560	arg1	pseudomallei					534:545	B. pseudomallei	531:545	B. pseudomallei	531:545	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	3	49	theme	causative	552:560	arg1	agent					562:566	the causative agent	548:566	the causative agent of melioidosis	548:581	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	2	50	theme	potent	483:488	arg1	LPSs					317:320	LPSs	317:320	LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis,	317:477	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	2	50	theme	potent	483:488	arg1	stimulators					497:507	potent immune stimulators	483:507	potent immune stimulators	483:507	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	4	51	theme	LPSs	860:863	arg1	characteristics					837:851	the structural and immunological characteristics	804:851	the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species	804:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	3	52	theme	antigenicities	770:783	arg1	understanding					724:736	understanding	724:736	understanding of their immune recognition and antigenicities	724:783	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	1	53	theme	bacteria	214:221	arg1	LPSs					191:194	LPSs	191:194	LPSs	191:194	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	1	53	theme	bacteria	214:221	arg1	Lipopolysaccharides					170:188	Lipopolysaccharides	170:188	Lipopolysaccharides (LPSs) of Gram-negative bacteria	170:221	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	3	54	theme	recognition	754:764	arg1	understanding					724:736	understanding	724:736	understanding of their immune recognition and antigenicities	724:783	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	6	55	theme	phosphate	1391:1399	arg1	groups					1401:1406	both terminal phosphate groups	1377:1406	both terminal phosphate groups	1377:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	8	56	theme	other	1760:1764	arg1	species					1779:1785	other Burkholderia species	1760:1785	other Burkholderia species	1760:1785	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	1	57	theme	lipid	232:236	arg1	OPS					269:271	OPS	269:271	OPS	269:271	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	1	57	theme	lipid	232:236	arg1	A					238:238	lipid A	232:238	lipid A	232:238	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	10	58	from	characteristics	1973:1987	arg1	species					2012:2018	Burkholderia species	1999:2018	Burkholderia species	1999:2018	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	5	59	theme	SDS-PAGE	1100:1107	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	10	60	from	LPS	1992:1994	arg1	species					2012:2018	Burkholderia species	1999:2018	Burkholderia species	1999:2018	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	5	61	theme	gas	1075:1077	arg1	chromatography					1079:1092	gas chromatography	1075:1092	gas chromatography (GC)	1075:1097	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	61	theme	gas	1075:1077	arg1	GC					1095:1096	GC	1095:1096	GC	1095:1096	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	7	62	from	m/z	1474:1476	arg1	differences					1459:1469	mass differences	1454:1469	mass differences at m/z 1,511, 1,642, 1,773, and 1,926	1454:1507	The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition.
31548320	7	63	theme	lipid	1413:1417	arg1	A					1419:1419	The lipid A	1409:1419	The lipid A	1409:1419	The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition.
31548320	8	64	theme	similar	1640:1646	arg1	patterns					1659:1666	similar LPS ladder patterns	1640:1666	similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei	1640:1719	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	10	65	theme	serological	2052:2062	arg1	diagnostics					2064:2074	serological diagnostics	2052:2074	serological diagnostics	2052:2074	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	0	66	theme	Toll-Like	97:105	arg1	Receptor					107:114	Toll-Like Receptor 4	97:116	Toll-Like Receptor 4 Activation	97:127	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	10	67	from	species	2012:2018	arg1	characteristics					1973:1987	the characteristics	1969:1987	the characteristics of LPS in Burkholderia species	1969:2018	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	4	68	attach	isolated	865:872	arg2	LPSs					860:863	the LPSs	856:863	the LPSs isolated from eight Burkholderia species	856:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	4	68	attach	isolated	865:872	arg1	species					898:904	eight Burkholderia species	879:904	eight Burkholderia species	879:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	5	69	theme	stimulation	1138:1148	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	70	theme	Matrix-assisted	982:996	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	4	71	theme	other	961:965	arg1	species					973:979	the other seven species	957:979	the other seven species	957:979	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	8	72	theme	species	1552:1558	arg1	LPSs					1540:1543	LPSs	1540:1543	LPSs of all species	1540:1558	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	5	73	theme	Toll-like	1110:1118	arg1	receptor					1120:1127	Toll-like receptor 4	1110:1129	Toll-like receptor 4 (TLR4) stimulation	1110:1148	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	73	theme	Toll-like	1110:1118	arg1	TLR4					1132:1135	TLR4	1132:1135	TLR4	1132:1135	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	74	theme	desorption	1004:1013	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	8	75	theme	TLR4-dependent	1568:1581	arg1	responses					1589:1597	TLR4-dependent NF-κB responses	1568:1597	TLR4-dependent NF-κB responses	1568:1597	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	10	76	used	used	1943:1946	arg2	findings					1927:1934	These findings	1921:1934	These findings	1921:1934	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	10	77	theme	Burkholderia	1999:2010	arg1	species					2012:2018	Burkholderia species	1999:2018	Burkholderia species	1999:2018	These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
31548320	9	78	theme	patient	1853:1859	arg1	sera					1861:1864	melioidosis patient sera	1841:1864	melioidosis patient sera	1841:1864	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	0	79	theme	Burkholderia	35:46	arg1	Species					48:54	Different Burkholderia Species	25:54	Different Burkholderia Species with Different Lipid A Structures	25:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	2	80	theme	Burkholderia	451:462	arg1	thailandensis					464:476	Burkholderia thailandensis	451:476	Burkholderia thailandensis	451:476	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	6	81	from	groups	1401:1406	arg1	residues					1365:1372	4-amino-4-deoxy-arabinose residues	1339:1372	4-amino-4-deoxy-arabinose residues at both terminal phosphate groups	1339:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	4	82	theme	immunological	823:835	arg1	characteristics					837:851	the structural and immunological characteristics	804:851	the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species	804:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	0	83	theme	Lipid	71:75	arg1	Structures					79:88	Different Lipid A Structures	61:88	Different Lipid A Structures	61:88	Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
31548320	9	84	theme	other	1893:1897	arg1	species					1912:1918	other Burkholderia species	1893:1918	other Burkholderia species	1893:1918	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	2	85	theme	pathogenic	340:349	arg1	species					351:357	the pathogenic species Burkholderia pseudomallei and Burkholderia mallei	336:407	the pathogenic species Burkholderia pseudomallei and Burkholderia mallei	336:407	Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators.
31548320	5	86	theme	analysis	1166:1173	arg1	ionization-time					1015:1029	Matrix-assisted laser desorption ionization-time	982:1029	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis	982:1173	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	9	87	theme	species	1912:1918	arg1	OPSs					1885:1888	OPSs	1885:1888	OPSs of other Burkholderia species	1885:1918	Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species.
31548320	6	88	theme	terminal	1382:1389	arg1	groups					1401:1406	both terminal phosphate groups	1377:1406	both terminal phosphate groups	1377:1406	MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups.
31548320	3	89	theme	LPS	514:516	arg1	structure					518:526	The LPS structure	510:526	The LPS structure of B. pseudomallei, the causative agent of melioidosis,	510:582	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
31548320	1	90	theme	core	241:244	arg1	components					274:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	lipid A, core, and O-polysaccharide (OPS) components	232:283	Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components.
31548320	5	91	theme	mass	1041:1044	arg1	spectrometry					1046:1057	flight mass spectrometry	1034:1057	flight mass spectrometry (MALDI-TOF MS)	1034:1072	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	5	91	theme	mass	1041:1044	arg1	MS					1070:1071	MALDI-TOF MS	1060:1071	MALDI-TOF MS	1060:1071	Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species.
31548320	8	92	theme	ladder	1652:1657	arg1	patterns					1659:1666	similar LPS ladder patterns	1640:1666	similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei	1640:1719	LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species.
31548320	4	93	theme	structural	808:817	arg1	characteristics					837:851	the structural and immunological characteristics	804:851	the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species	804:904	Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species.
31548320	3	94	dep	other	663:667	arg1	related					670:676	related	670:676	related	670:676	The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities.
30707395	8	0	theme	Monosaccharide	982:995	arg1	analysis					1009:1016	Monosaccharide composition analysis	982:1016	Monosaccharide composition analysis	982:1016	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	6	1	theme	Tubby-like	719:728	arg1	gene					730:733	a Tubby-like gene	717:733	a Tubby-like gene	717:733	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	6	1	theme	Tubby-like	719:728	arg1	protein					747:753	Tubby-like protein 2	736:755	Tubby-like protein 2 (TLP2)	736:762	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	10	2	theme	subunit	1352:1358	arg1	NF-YC3					1364:1369	NF-YC3	1364:1369	NF-YC3	1364:1369	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	10	2	theme	subunit	1352:1358	arg1	C3					1360:1361	Nuclear Factor Y subunit C3	1335:1361	Nuclear Factor Y subunit C3 (NF-YC3)	1335:1370	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	8	3	theme	acid	1076:1079	arg1	sugars					1052:1057	total sugars	1046:1057	total sugars	1046:1057	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	3	theme	acid	1076:1079	arg1	acid					1076:1079	galacturonic acid	1063:1079	galacturonic acid (GalA)	1063:1086	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	3	theme	acid	1076:1079	arg1	GalA					1082:1085	GalA	1082:1085	GalA	1082:1085	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	3	theme	acid	1076:1079	arg1	amount					1036:1041	the amount	1032:1041	the amount of total sugars and galacturonic acid (GalA)	1032:1086	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	3	4	theme	TLP2	330:333	arg1	function					340:347	TLP2 gene function	330:347	TLP2 gene function	330:347	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	9	5	theme	tlp2-1	1253:1258	arg1	mutant					1260:1265	the tlp2-1 mutant	1249:1265	the tlp2-1 mutant	1249:1265	Immunolabelling and dot immunoblotting analysis showed that unesterified HG decreased in the tlp2-1 mutant.
30707395	8	6	theme	composition	997:1007	arg1	analysis					1009:1016	Monosaccharide composition analysis	982:1016	Monosaccharide composition analysis	982:1016	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	7	7	theme	red	857:859	arg1	staining					866:873	Ruthenium red (RR) staining	847:873	Ruthenium red (RR) staining of the seeds	847:886	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	10	8	theme	transcription	1389:1401	arg1	factor					1403:1408	a transcription factor	1387:1408	a transcription factor	1387:1408	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	8	9	theme	adherent	1119:1126	arg1	AM					1138:1139	AM	1138:1139	AM	1138:1139	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	9	theme	adherent	1119:1126	arg1	mucilage					1128:1135	the adherent mucilage	1115:1135	the adherent mucilage (AM) of tlp2-1 mutant	1115:1157	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	5	10	theme	Arabidopsis	665:675	arg1	mucilage					687:694	the Arabidopsis seed coat mucilage	661:694	the Arabidopsis seed coat mucilage	661:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	6	11	theme	developing	791:800	arg1	siliques					802:809	developing siliques	791:809	developing siliques	791:809	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	12	12	theme	UGE1	1635:1638	arg1	activation					1621:1630	the positive activation	1608:1630	the positive activation of UGE1	1608:1638	In all, it is concluded that TLP2 could regulate the biosynthesis of HG possibly through the positive activation of UGE1.
30707395	10	13	theme	Nuclear	1335:1341	arg1	NF-YC3					1364:1369	NF-YC3	1364:1369	NF-YC3	1364:1369	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	10	13	theme	Nuclear	1335:1341	arg1	C3					1360:1361	Nuclear Factor Y subunit C3	1335:1361	Nuclear Factor Y subunit C3 (NF-YC3)	1335:1370	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	5	14	theme	seed	677:680	arg1	mucilage					687:694	the Arabidopsis seed coat mucilage	661:694	the Arabidopsis seed coat mucilage	661:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	7	15	theme	tlp2-1	917:922	arg1	mutant					924:929	tlp2-1 mutant	917:929	tlp2-1 mutant	917:929	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	3	16	from	network	384:390	arg1	thaliana					430:437	Arabidopsis thaliana	418:437	Arabidopsis thaliana	418:437	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	7	17	theme	mutant	924:929	arg1	mucilage					905:912	defective mucilage	895:912	defective mucilage of tlp2-1 mutant	895:929	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	5	18	theme	major	624:628	arg1	homogalacturonan					594:609	homogalacturonan	594:609	homogalacturonan (HG)	594:614	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	18	theme	major	624:628	arg1	Rhamnogalacturonan					562:579	Rhamnogalacturonan I	562:581	Rhamnogalacturonan I (RG-I)	562:588	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	18	theme	major	624:628	arg1	constituent					646:656	the major polysaccharides constituent	620:656	the major polysaccharides constituent of the Arabidopsis seed coat mucilage	620:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	0	19	theme	Tubby-like	0:9	arg1	Protein					11:17	Tubby-like Protein 2	0:19	Tubby-like Protein 2	0:19	Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
30707395	12	20	theme	positive	1612:1619	arg1	activation					1621:1630	the positive activation	1608:1630	the positive activation of UGE1	1608:1638	In all, it is concluded that TLP2 could regulate the biosynthesis of HG possibly through the positive activation of UGE1.
30707395	5	21	theme	polysaccharides	630:644	arg1	homogalacturonan					594:609	homogalacturonan	594:609	homogalacturonan (HG)	594:614	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	21	theme	polysaccharides	630:644	arg1	Rhamnogalacturonan					562:579	Rhamnogalacturonan I	562:581	Rhamnogalacturonan I (RG-I)	562:588	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	21	theme	polysaccharides	630:644	arg1	constituent					646:656	the major polysaccharides constituent	620:656	the major polysaccharides constituent of the Arabidopsis seed coat mucilage	620:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	0	22	theme	coat	81:84	arg1	mucilage					86:93	Arabidopsis seed coat mucilage	64:93	Arabidopsis seed coat mucilage	64:93	Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
30707395	4	23	theme	model	487:491	arg1	system					493:498	an excellent model system	474:498	an excellent model system to study the biosynthesis, function and regulation of pectin	474:559	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	23	theme	model	487:491	arg1	mucilage					462:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	1	24	theme	HG	184:185	arg1	biosynthesis					187:198	HG biosynthesis	184:198	HG biosynthesis	184:198	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	3	25	theme	pectin	395:400	arg1	biosynthesis					402:413	pectin biosynthesis	395:413	pectin biosynthesis	395:413	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	8	26	theme	galacturonic	1063:1074	arg1	acid					1076:1079	galacturonic acid	1063:1079	galacturonic acid (GalA)	1063:1086	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	26	theme	galacturonic	1063:1074	arg1	GalA					1082:1085	GalA	1082:1085	GalA	1082:1085	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	1	27	theme	possible	98:105	arg1	factor					121:126	A possible transcription factor	96:126	A possible transcription factor TLP2	96:131	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	11	28	theme	RNA-sequence	1411:1422	arg1	assays					1444:1449	RNA-sequence and transactivation assays	1411:1449	RNA-sequence and transactivation assays	1411:1449	RNA-sequence and transactivation assays revealed that TLP2 could activate UDP-glucose 4-epimerase 1 (UGE1).
30707395	1	29	theme	biosynthesis	187:198	arg1	regulation					170:179	the regulation	166:179	the regulation of HG biosynthesis in Arabidopsis seed mucilage	166:227	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	3	30	theme	regulatory	373:382	arg1	network					384:390	the regulatory network	369:390	the regulatory network of pectin biosynthesis in Arabidopsis thaliana	369:437	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	8	31	theme	total	1046:1050	arg1	sugars					1052:1057	total sugars	1046:1057	total sugars	1046:1057	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	7	32	theme	defective	895:903	arg1	mucilage					905:912	defective mucilage	895:912	defective mucilage of tlp2-1 mutant	895:929	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	1	33	theme	transcription	107:119	arg1	factor					121:126	A possible transcription factor	96:126	A possible transcription factor TLP2	96:131	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	3	34	theme	function	340:347	arg1	discovery					317:325	The discovery	313:325	The discovery of TLP2 gene function	313:347	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	11	35	theme	UDP-glucose	1485:1495	arg1	4-epimerase					1497:1507	UDP-glucose 4-epimerase 1	1485:1509	UDP-glucose 4-epimerase 1 (UGE1)	1485:1516	RNA-sequence and transactivation assays revealed that TLP2 could activate UDP-glucose 4-epimerase 1 (UGE1).
30707395	11	35	theme	UDP-glucose	1485:1495	arg1	UGE1					1512:1515	UGE1	1512:1515	UGE1	1512:1515	RNA-sequence and transactivation assays revealed that TLP2 could activate UDP-glucose 4-epimerase 1 (UGE1).
30707395	9	36	theme	dot	1180:1182	arg1	analysis					1199:1206	dot immunoblotting analysis	1180:1206	dot immunoblotting analysis	1180:1206	Immunolabelling and dot immunoblotting analysis showed that unesterified HG decreased in the tlp2-1 mutant.
30707395	1	37	theme	factor	121:126	arg1	TLP2					128:131	A possible transcription factor TLP2	96:131	A possible transcription factor TLP2	96:131	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	12	38	theme	HG	1588:1589	arg1	biosynthesis					1572:1583	the biosynthesis	1568:1583	the biosynthesis of HG	1568:1589	In all, it is concluded that TLP2 could regulate the biosynthesis of HG possibly through the positive activation of UGE1.
30707395	1	39	theme	Arabidopsis	203:213	arg1	mucilage					220:227	Arabidopsis seed mucilage	203:227	Arabidopsis seed mucilage	203:227	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	3	40	theme	Arabidopsis	418:428	arg1	thaliana					430:437	Arabidopsis thaliana	418:437	Arabidopsis thaliana	418:437	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	9	41	theme	immunoblotting	1184:1197	arg1	analysis					1199:1206	dot immunoblotting analysis	1180:1206	dot immunoblotting analysis	1180:1206	Immunolabelling and dot immunoblotting analysis showed that unesterified HG decreased in the tlp2-1 mutant.
30707395	1	42	theme	seed	215:218	arg1	mucilage					220:227	Arabidopsis seed mucilage	203:227	Arabidopsis seed mucilage	203:227	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
30707395	0	43	theme	homogalacturonan	31:46	arg1	biosynthesis					48:59	homogalacturonan biosynthesis	31:59	homogalacturonan biosynthesis	31:59	Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
30707395	8	44	theme	sugars	1052:1057	arg1	sugars					1052:1057	total sugars	1046:1057	total sugars	1046:1057	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	44	theme	sugars	1052:1057	arg1	acid					1076:1079	galacturonic acid	1063:1079	galacturonic acid (GalA)	1063:1086	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	44	theme	sugars	1052:1057	arg1	GalA					1082:1085	GalA	1082:1085	GalA	1082:1085	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	44	theme	sugars	1052:1057	arg1	amount					1036:1041	the amount	1032:1041	the amount of total sugars and galacturonic acid (GalA)	1032:1086	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	4	45	theme	excellent	477:485	arg1	system					493:498	an excellent model system	474:498	an excellent model system to study the biosynthesis, function and regulation of pectin	474:559	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	45	theme	excellent	477:485	arg1	mucilage					462:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	8	46	theme	tlp2-1	1145:1150	arg1	mutant					1152:1157	tlp2-1 mutant	1145:1157	tlp2-1 mutant	1145:1157	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	3	47	theme	biosynthesis	402:413	arg1	network					384:390	the regulatory network	369:390	the regulatory network of pectin biosynthesis in Arabidopsis thaliana	369:437	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	5	48	theme	coat	682:685	arg1	mucilage					687:694	the Arabidopsis seed coat mucilage	661:694	the Arabidopsis seed coat mucilage	661:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	0	49	theme	seed	76:79	arg1	mucilage					86:93	Arabidopsis seed coat mucilage	64:93	Arabidopsis seed coat mucilage	64:93	Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
30707395	4	50	theme	pectin	554:559	arg1	biosynthesis					513:524	biosynthesis	513:524	biosynthesis	513:524	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	50	theme	pectin	554:559	arg1	regulation					540:549	regulation	540:549	regulation	540:549	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	50	theme	pectin	554:559	arg1	function					527:534	function	527:534	function	527:534	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	5	51	theme	mucilage	687:694	arg1	homogalacturonan					594:609	homogalacturonan	594:609	homogalacturonan (HG)	594:614	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	51	theme	mucilage	687:694	arg1	Rhamnogalacturonan					562:579	Rhamnogalacturonan I	562:581	Rhamnogalacturonan I (RG-I)	562:588	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	5	51	theme	mucilage	687:694	arg1	constituent					646:656	the major polysaccharides constituent	620:656	the major polysaccharides constituent of the Arabidopsis seed coat mucilage	620:694	Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage.
30707395	4	52	theme	Arabidopsis	440:450	arg1	system					493:498	an excellent model system	474:498	an excellent model system to study the biosynthesis, function and regulation of pectin	474:559	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	52	theme	Arabidopsis	440:450	arg1	mucilage					462:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	9	53	theme	unesterified	1220:1231	arg1	HG					1233:1234	unesterified HG	1220:1234	unesterified HG	1220:1234	Immunolabelling and dot immunoblotting analysis showed that unesterified HG decreased in the tlp2-1 mutant.
30707395	2	54	theme	plasma	269:274	arg1	membrane					276:283	plasma membrane	269:283	plasma membrane	269:283	TLP2 can translocate into nucleus from plasma membrane by interacting with NF-YC3.
30707395	7	55	theme	vigorous	937:944	arg1	shaking					946:952	vigorous shaking	937:952	vigorous shaking	937:952	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	7	56	theme	seeds	882:886	arg1	staining					866:873	Ruthenium red (RR) staining	847:873	Ruthenium red (RR) staining of the seeds	847:886	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	4	57	theme	coat	457:460	arg1	system					493:498	an excellent model system	474:498	an excellent model system to study the biosynthesis, function and regulation of pectin	474:559	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	57	theme	coat	457:460	arg1	mucilage					462:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	6	58	theme	Tubby-like	736:745	arg1	gene					730:733	a Tubby-like gene	717:733	a Tubby-like gene	717:733	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	6	58	theme	Tubby-like	736:745	arg1	protein					747:753	Tubby-like protein 2	736:755	Tubby-like protein 2 (TLP2)	736:762	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	6	58	theme	Tubby-like	736:745	arg1	TLP2					758:761	TLP2	758:761	TLP2	758:761	Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced.
30707395	11	59	theme	transactivation	1428:1442	arg1	assays					1444:1449	RNA-sequence and transactivation assays	1411:1449	RNA-sequence and transactivation assays	1411:1449	RNA-sequence and transactivation assays revealed that TLP2 could activate UDP-glucose 4-epimerase 1 (UGE1).
30707395	8	60	theme	mutant	1152:1157	arg1	AM					1138:1139	AM	1138:1139	AM	1138:1139	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	8	60	theme	mutant	1152:1157	arg1	mucilage					1128:1135	the adherent mucilage	1115:1135	the adherent mucilage (AM) of tlp2-1 mutant	1115:1157	Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant.
30707395	3	61	theme	gene	335:338	arg1	function					340:347	TLP2 gene function	330:347	TLP2 gene function	330:347	The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana.
30707395	4	62	theme	seed	452:455	arg1	system					493:498	an excellent model system	474:498	an excellent model system to study the biosynthesis, function and regulation of pectin	474:559	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	4	62	theme	seed	452:455	arg1	mucilage					462:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage	440:469	Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin.
30707395	7	63	theme	wild	966:969	arg1	WT					977:978	WT	977:978	WT	977:978	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	7	63	theme	wild	966:969	arg1	type					971:974	wild type	966:974	wild type (WT)	966:979	Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT).
30707395	10	64	theme	Y	1350:1350	arg1	NF-YC3					1364:1369	NF-YC3	1364:1369	NF-YC3	1364:1369	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	10	64	theme	Y	1350:1350	arg1	C3					1360:1361	Nuclear Factor Y subunit C3	1335:1361	Nuclear Factor Y subunit C3 (NF-YC3)	1335:1370	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	10	65	theme	Factor	1343:1348	arg1	NF-YC3					1364:1369	NF-YC3	1364:1369	NF-YC3	1364:1369	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	10	65	theme	Factor	1343:1348	arg1	C3					1360:1361	Nuclear Factor Y subunit C3	1335:1361	Nuclear Factor Y subunit C3 (NF-YC3)	1335:1370	Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor.
30707395	0	66	theme	Arabidopsis	64:74	arg1	mucilage					86:93	Arabidopsis seed coat mucilage	64:93	Arabidopsis seed coat mucilage	64:93	Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
30707395	1	67	from	regulation	170:179	arg1	mucilage					220:227	Arabidopsis seed mucilage	203:227	Arabidopsis seed mucilage	203:227	A possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage.
31707684	0	0	theme	bunch	60:64	arg1	explosion					16:24	steam explosion	10:24	steam explosion of oil palm frond and empty fruit bunch	10:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	2	1	theme	P	397:397	arg1	<					399:399	P < 0.01	397:404	P < 0.01	397:404	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	2	1	theme	P	397:397	arg1	ADF					392:394	ADF	392:394	ADF (P < 0.01)	392:405	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	5	2	contain	had	711:713	arg2	tendency					717:724	a tendency	715:724	a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082)	715:802	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	2	contain	had	711:713	arg1	explosion					701:709	Steam explosion	695:709	Steam explosion	695:709	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	1	3	theme	OPF	209:211	arg1	explosion					180:188	steam explosion	174:188	steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB)	174:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	0	4	theme	fruit	54:58	arg1	bunch					60:64	oil palm frond and empty fruit bunch	29:64	oil palm frond and empty fruit bunch	29:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	0	5	theme	nutrient	69:76	arg1	composition					78:88	nutrient composition	69:88	nutrient composition	69:88	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	0	6	from	Effect	0:5	arg1	characteristics					115:129	ruminal fermentation characteristics	94:129	ruminal fermentation characteristics	94:129	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	0	6	from	Effect	0:5	arg1	composition					78:88	nutrient composition	69:88	nutrient composition	69:88	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	5	7	theme	acids	779:783	arg1	concentration					741:753	the concentration	737:753	the concentration of total volatile fatty acids (TVFA)	737:790	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	8	dep	tendency	717:724	arg1	P					793:793	P	793:793	P = 0.082	793:801	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	1	9	theme	fermentation	287:298	arg1	characteristics					300:314	ruminal fermentation characteristics	279:314	ruminal fermentation characteristics	279:314	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	4	10	theme	EFB	613:615	arg1	capacity					593:600	72-h gas production capacity	573:600	72-h gas production capacity of OPF and EFB	573:615	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	1	11	theme	oil	218:220	arg1	EFB					246:248	EFB	246:248	EFB	246:248	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	11	theme	oil	218:220	arg1	bunch					239:243	oil palm empty fruit bunch	218:243	oil palm empty fruit bunch (EFB)	218:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	2	12	theme	P	435:435	arg1	content					426:432	hemicellulose content	412:432	hemicellulose content (P < 0.01)	412:443	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	2	12	theme	P	435:435	arg1	<					437:437	P < 0.01	435:442	P < 0.01	435:442	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	4	13	theme	12.60and	630:637	arg1	P					647:647	P < 0.01	647:654	P < 0.01	647:654	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	4	13	theme	12.60and	630:637	arg1	%					644:644	12.60and 85.06%	630:644	12.60and 85.06% (P < 0.01)	630:655	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	5	14	theme	Steam	695:699	arg1	explosion					701:709	Steam explosion	695:709	Steam explosion	695:709	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	15	theme	=	795:795	arg1	P					793:793	P	793:793	P = 0.082	793:801	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	6	16	theme	TVFA	928:931	arg1	concentration					933:945	TVFA concentration	928:945	TVFA concentration	928:945	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	4	17	theme	steam	678:682	arg1	explosion					684:692	steam explosion	678:692	steam explosion	678:692	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	5	18	theme	total	758:762	arg1	TVFA					786:789	TVFA	786:789	TVFA	786:789	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	18	theme	total	758:762	arg1	acids					779:783	total volatile fatty acids	758:783	total volatile fatty acids (TVFA)	758:790	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	4	19	theme	In	529:530	arg1	results					551:557	In vitro fermentation results	529:557	In vitro fermentation results	529:557	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	2	20	theme	P	381:381	arg1	NDF					376:378	NDF	376:378	NDF (P < 0.01)	376:389	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	2	20	theme	P	381:381	arg1	<					383:383	P < 0.01	381:388	P < 0.01	381:388	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	1	21	theme	palm	222:225	arg1	EFB					246:248	EFB	246:248	EFB	246:248	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	21	theme	palm	222:225	arg1	bunch					239:243	oil palm empty fruit bunch	218:243	oil palm empty fruit bunch (EFB)	218:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	5	22	theme	volatile	764:771	arg1	TVFA					786:789	TVFA	786:789	TVFA	786:789	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	22	theme	volatile	764:771	arg1	acids					779:783	total volatile fatty acids	758:783	total volatile fatty acids (TVFA)	758:790	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	3	23	theme	OPF	516:518	arg1	value					507:511	the effective energy value	486:511	the effective energy value of OPF and EFB	486:526	Steam explosion improved the effective energy value of OPF and EFB.
31707684	0	24	theme	explosion	16:24	arg1	Effect					0:5	Effect	0:5	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.	0:130	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	1	25	theme	empty	227:231	arg1	EFB					246:248	EFB	246:248	EFB	246:248	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	25	theme	empty	227:231	arg1	bunch					239:243	oil palm empty fruit bunch	218:243	oil palm empty fruit bunch (EFB)	218:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	0	26	theme	fermentation	102:113	arg1	characteristics					115:129	ruminal fermentation characteristics	94:129	ruminal fermentation characteristics	94:129	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	6	27	theme	gas	909:911	arg1	production					913:922	gas production	909:922	gas production	909:922	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	5	28	theme	fatty	773:777	arg1	TVFA					786:789	TVFA	786:789	TVFA	786:789	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	5	28	theme	fatty	773:777	arg1	acids					779:783	total volatile fatty acids	758:783	total volatile fatty acids (TVFA)	758:790	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	6	29	theme	OPF	839:841	arg1	explosion					826:834	steam explosion	820:834	steam explosion of OPF and EFB	820:849	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	0	30	theme	steam	10:14	arg1	explosion					16:24	steam explosion	10:24	steam explosion of oil palm frond and empty fruit bunch	10:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	1	31	theme	fruit	233:237	arg1	EFB					246:248	EFB	246:248	EFB	246:248	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	31	theme	fruit	233:237	arg1	bunch					239:243	oil palm empty fruit bunch	218:243	oil palm empty fruit bunch (EFB)	218:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	0	32	theme	ruminal	94:100	arg1	characteristics					115:129	ruminal fermentation characteristics	94:129	ruminal fermentation characteristics	94:129	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	4	33	theme	OPF	605:607	arg1	capacity					593:600	72-h gas production capacity	573:600	72-h gas production capacity of OPF and EFB	573:615	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	1	34	theme	steam	174:178	arg1	explosion					180:188	steam explosion	174:188	steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB)	174:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	0	35	theme	oil	29:31	arg1	bunch					60:64	oil palm frond and empty fruit bunch	29:64	oil palm frond and empty fruit bunch	29:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	1	36	theme	bunch	239:243	arg1	explosion					180:188	steam explosion	174:188	steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB)	174:249	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	2	37	theme	steam	350:354	arg1	explosion					356:364	steam explosion	350:364	steam explosion	350:364	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	1	38	theme	explosion	180:188	arg1	effect					164:169	the effect	160:169	the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics	160:314	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	6	39	theme	EFB	847:849	arg1	explosion					826:834	steam explosion	820:834	steam explosion of OPF and EFB	820:849	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	3	40	theme	energy	500:505	arg1	value					507:511	the effective energy value	486:511	the effective energy value of OPF and EFB	486:526	Steam explosion improved the effective energy value of OPF and EFB.
31707684	3	41	theme	Steam	461:465	arg1	explosion					467:475	Steam explosion	461:475	Steam explosion	461:475	Steam explosion improved the effective energy value of OPF and EFB.
31707684	5	42	dep	=	795:795	arg1	0.082					797:801	0.082	797:801	0.082	797:801	Steam explosion had a tendency to improve the concentration of total volatile fatty acids (TVFA) (P = 0.082).
31707684	0	43	theme	frond	38:42	arg1	bunch					60:64	oil palm frond and empty fruit bunch	29:64	oil palm frond and empty fruit bunch	29:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	2	44	theme	hemicellulose	412:424	arg1	content					426:432	hemicellulose content	412:432	hemicellulose content (P < 0.01)	412:443	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	2	44	theme	hemicellulose	412:424	arg1	<					437:437	P < 0.01	435:442	P < 0.01	435:442	The results showed that steam explosion decreased NDF (P < 0.01), ADF (P < 0.01), and hemicellulose content (P < 0.01) in OPF and EFB.
31707684	6	45	theme	steam	820:824	arg1	explosion					826:834	steam explosion	820:834	steam explosion of OPF and EFB	820:849	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	4	46	theme	72-h	573:576	arg1	capacity					593:600	72-h gas production capacity	573:600	72-h gas production capacity of OPF and EFB	573:615	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	0	47	theme	palm	33:36	arg1	bunch					60:64	oil palm frond and empty fruit bunch	29:64	oil palm frond and empty fruit bunch	29:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	1	48	theme	ruminal	279:285	arg1	characteristics					300:314	ruminal fermentation characteristics	279:314	ruminal fermentation characteristics	279:314	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	4	49	dep	In	529:530	arg1	vitro					532:536	vitro	532:536	vitro	532:536	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	4	50	theme	<	649:649	arg1	P					647:647	P < 0.01	647:654	P < 0.01	647:654	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	4	50	theme	<	649:649	arg1	%					644:644	12.60and 85.06%	630:644	12.60and 85.06% (P < 0.01)	630:655	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	4	51	theme	production	582:591	arg1	capacity					593:600	72-h gas production capacity	573:600	72-h gas production capacity of OPF and EFB	573:615	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	6	52	theme	hemicellulose	873:885	arg1	content					887:893	hemicellulose content	873:893	hemicellulose content	873:893	In conclusion, steam explosion of OPF and EFB reduced NDF, ADF, and hemicellulose content and increased gas production and TVFA concentration.
31707684	1	53	theme	oil	193:195	arg1	OPF					209:211	oil palm frond (OPF)	193:212	oil palm frond (OPF)	193:212	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	3	54	theme	effective	490:498	arg1	value					507:511	the effective energy value	486:511	the effective energy value of OPF and EFB	486:526	Steam explosion improved the effective energy value of OPF and EFB.
31707684	4	55	theme	gas	578:580	arg1	capacity					593:600	72-h gas production capacity	573:600	72-h gas production capacity of OPF and EFB	573:615	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	1	56	theme	palm	197:200	arg1	OPF					209:211	oil palm frond (OPF)	193:212	oil palm frond (OPF)	193:212	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	0	57	theme	empty	48:52	arg1	bunch					60:64	oil palm frond and empty fruit bunch	29:64	oil palm frond and empty fruit bunch	29:64	Effect of steam explosion of oil palm frond and empty fruit bunch on nutrient composition and ruminal fermentation characteristics.
31707684	3	58	theme	EFB	524:526	arg1	value					507:511	the effective energy value	486:511	the effective energy value of OPF and EFB	486:526	Steam explosion improved the effective energy value of OPF and EFB.
31707684	1	59	from	effect	164:169	arg1	characteristics					300:314	ruminal fermentation characteristics	279:314	ruminal fermentation characteristics	279:314	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	59	from	effect	164:169	arg1	composition					263:273	nutrient composition	254:273	nutrient composition	254:273	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	4	60	theme	fermentation	538:549	arg1	results					551:557	In vitro fermentation results	529:557	In vitro fermentation results	529:557	In vitro fermentation results revealed that 72-h gas production capacity of OPF and EFB increased by 12.60and 85.06% (P < 0.01), respectively, after steam explosion.
31707684	1	61	theme	frond	202:206	arg1	OPF					209:211	oil palm frond (OPF)	193:212	oil palm frond (OPF)	193:212	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31707684	1	62	theme	nutrient	254:261	arg1	composition					263:273	nutrient composition	254:273	nutrient composition	254:273	In this study, we evaluated the effect of steam explosion of oil palm frond (OPF) and oil palm empty fruit bunch (EFB) on nutrient composition and ruminal fermentation characteristics in vitro.
31029355	5	0	theme	suitable	908:915	arg1	performance					917:927	the suitable performance	904:927	the suitable performance	904:927	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	7	1	theme	self-healing	1177:1188	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	1	theme	self-healing	1177:1188	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	5	2	theme	MGMs	861:864	arg1	concentration					835:847	a concentration	833:847	a concentration of 30 mg/mL MGMs	833:864	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	1	3	theme	injectable	170:179	arg1	scaffolds					190:198	injectable hydrogel scaffolds	170:198	injectable hydrogel scaffolds	170:198	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	6	4	theme	in	1057:1058	arg1	release					1071:1077	the sustained in vitro drug release	1043:1077	the sustained in vitro drug release	1043:1077	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	7	5	theme	drug	1285:1288	arg1	delivery					1290:1297	drug delivery	1285:1297	drug delivery	1285:1297	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	6	6	dep	in	1057:1058	arg1	vitro					1060:1064	vitro	1060:1064	vitro	1060:1064	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	7	7	theme	CEC-OAlg	1190:1197	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	7	theme	CEC-OAlg	1190:1197	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	1	8	theme	hydrogel	181:188	arg1	scaffolds					190:198	injectable hydrogel scaffolds	170:198	injectable hydrogel scaffolds	170:198	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	6	9	theme	sustained	1047:1055	arg1	release					1071:1077	the sustained in vitro drug release	1043:1077	the sustained in vitro drug release	1043:1077	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	7	10	theme	magnetic	1164:1171	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	10	theme	magnetic	1164:1171	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	4	11	theme	MGMs	797:800	arg1	effect					787:792	the effect	783:792	the effect of MGMs on the CEC-OAlg hydrogel	783:825	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	0	12	theme	drug	118:121	arg1	delivery					123:130	drug delivery	118:130	drug delivery	118:130	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	3	13	theme	biological	535:544	arg1	capabilities					546:557	the mechanical and biological capabilities	516:557	the mechanical and biological capabilities of hydrogel	516:569	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	5	14	theme	excellent	940:948	arg1	ability					963:969	excellent self-healing ability	940:969	excellent self-healing ability	940:969	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	1	15	theme	scaffolds	190:198	arg1	abilities					157:165	the abilities	153:165	the abilities of injectable hydrogel scaffolds	153:198	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	0	16	theme	gelatin	66:72	arg1	microspheres					74:85	gelatin microspheres	66:85	gelatin microspheres	66:85	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	4	17	from	effect	787:792	arg1	hydrogel					818:825	the CEC-OAlg hydrogel	805:825	the CEC-OAlg hydrogel	805:825	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	4	18	theme	swelling	685:692	arg1	ratio					694:698	swelling ratio	685:698	swelling ratio	685:698	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	7	19	contain	containing	1235:1244	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	19	contain	containing	1235:1244	arg2	5-Fu					1246:1249	5-Fu	1246:1249	5-Fu	1246:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	19	contain	containing	1235:1244	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	5	20	theme	physiological	977:989	arg1	condition					991:999	physiological condition	977:999	physiological condition	977:999	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	0	21	theme	Magnetic	0:7	arg1	hydrogel					44:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	1	22	theme	self-healing	203:214	arg1	hydrogel					234:241	a self-healing chitosan/alginate hydrogel	201:241	a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs)	201:296	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	0	23	theme	chitosan-alginate	26:42	arg1	hydrogel					44:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	1	24	theme	anti-cancer	315:325	arg1	delivery					332:339	anti-cancer drug delivery	315:339	anti-cancer drug delivery	315:339	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	1	25	theme	chitosan/alginate	216:232	arg1	hydrogel					234:241	a self-healing chitosan/alginate hydrogel	201:241	a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs)	201:296	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	0	26	theme	self-healing	13:24	arg1	hydrogel					44:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel	0:51	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	1	27	theme	drug	327:330	arg1	delivery					332:339	anti-cancer drug delivery	315:339	anti-cancer drug delivery	315:339	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	6	28	theme	drug	1066:1069	arg1	release					1071:1077	the sustained in vitro drug release	1043:1077	the sustained in vitro drug release	1043:1077	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	7	29	theme	tissue	1308:1313	arg1	engineering					1315:1325	soft tissue engineering	1303:1325	soft tissue engineering	1303:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	4	30	theme	compressive	714:724	arg1	modulus					726:732	compressive modulus	714:732	compressive modulus	714:732	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	7	31	theme	encapsulated	1217:1228	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	31	theme	encapsulated	1217:1228	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	5	32	theme	self-healing	950:961	arg1	ability					963:969	excellent self-healing ability	940:969	excellent self-healing ability	940:969	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	6	33	theme	control	1093:1099	arg1	MGMs					1101:1104	control MGMs	1093:1104	control MGMs	1093:1104	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	6	34	theme	CEC-OAlg	1110:1117	arg1	hydrogel					1119:1126	CEC-OAlg hydrogel	1110:1126	CEC-OAlg hydrogel	1110:1126	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	2	35	theme	oxidized	447:454	arg1	OAlg					466:469	OAlg	466:469	OAlg	466:469	The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction.
31029355	2	35	theme	oxidized	447:454	arg1	alginate					456:463	oxidized alginate	447:463	oxidized alginate (OAlg)	447:470	The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction.
31029355	3	36	theme	emulsion	630:637	arg1	method					653:658	an emulsion cross-linking method	627:658	an emulsion cross-linking method	627:658	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	5	37	theme	composite	871:879	arg1	hydrogel					881:888	the composite hydrogel	867:888	the composite hydrogel	867:888	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	7	38	theme	hydrogel	1199:1206	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	38	theme	hydrogel	1199:1206	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	1	39	theme	soft	345:348	arg1	engineering					357:367	soft tissue engineering	345:367	soft tissue engineering	345:367	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	6	40	theme	composite	1017:1025	arg1	hydrogel					1027:1034	this composite hydrogel	1012:1034	this composite hydrogel	1012:1034	Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel.
31029355	3	41	theme	mechanical	520:529	arg1	capabilities					546:557	the mechanical and biological capabilities	516:557	the mechanical and biological capabilities of hydrogel	516:569	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	2	42	theme	Schiff-base	480:490	arg1	reaction					492:499	the Schiff-base reaction	476:499	the Schiff-base reaction	476:499	The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction.
31029355	1	43	theme	tissue	350:355	arg1	engineering					357:367	soft tissue engineering	345:367	soft tissue engineering	345:367	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	4	44	dep	In	661:662	arg1	vitro					664:668	vitro	664:668	vitro	664:668	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	4	45	theme	rheological	738:748	arg1	behaviors					750:758	rheological behaviors	738:758	rheological behaviors	738:758	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	3	46	contain	containing	577:586	arg2	5-Fu					604:607	5-Fu	604:607	5-Fu	604:607	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	3	46	contain	containing	577:586	arg2	5-fluorouracil					588:601	5-fluorouracil	588:601	5-fluorouracil (5-Fu)	588:608	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	3	46	contain	containing	577:586	arg1	MGMs					572:575	MGMs	572:575	MGMs containing 5-fluorouracil (5-Fu)	572:608	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	7	47	theme	scaffold	1208:1215	arg1	platform					1272:1279	a platform	1270:1279	a platform for drug delivery and soft tissue engineering	1270:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	7	47	theme	scaffold	1208:1215	arg1	MGMs					1230:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs	1159:1233	this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu	1159:1249	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31029355	1	48	theme	magnetic	261:268	arg1	microspheres					278:289	magnetic gelatin microspheres	261:289	magnetic gelatin microspheres (MGMs)	261:296	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	1	48	theme	magnetic	261:268	arg1	MGMs					292:295	MGMs	292:295	MGMs	292:295	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	4	49	theme	CEC-OAlg	809:816	arg1	hydrogel					818:825	the CEC-OAlg hydrogel	805:825	the CEC-OAlg hydrogel	805:825	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	1	50	theme	gelatin	270:276	arg1	microspheres					278:289	magnetic gelatin microspheres	261:289	magnetic gelatin microspheres (MGMs)	261:296	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	1	50	theme	gelatin	270:276	arg1	MGMs					292:295	MGMs	292:295	MGMs	292:295	In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering.
31029355	0	51	theme	covalent	91:98	arg1	cross-linking					100:112	covalent cross-linking	91:112	covalent cross-linking for drug delivery	91:130	Magnetic and self-healing chitosan-alginate hydrogel encapsulated gelatin microspheres via covalent cross-linking for drug delivery.
31029355	3	52	theme	cross-linking	639:651	arg1	method					653:658	an emulsion cross-linking method	627:658	an emulsion cross-linking method	627:658	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	4	53	theme	gelation	670:677	arg1	time					679:682	In vitro gelation time	661:682	In vitro gelation time	661:682	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	2	54	theme	carboxyethyl	415:426	arg1	CEC					438:440	CEC	438:440	CEC	438:440	The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction.
31029355	2	54	theme	carboxyethyl	415:426	arg1	chitosan					428:435	carboxyethyl chitosan	415:435	carboxyethyl chitosan (CEC)	415:441	The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction.
31029355	4	55	theme	In	661:662	arg1	time					679:682	In vitro gelation time	661:682	In vitro gelation time	661:682	In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel.
31029355	5	56	theme	30 mg/mL	852:859	arg1	MGMs					861:864	30 mg/mL MGMs	852:864	30 mg/mL MGMs	852:864	With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition.
31029355	3	57	theme	hydrogel	562:569	arg1	capabilities					546:557	the mechanical and biological capabilities	516:557	the mechanical and biological capabilities of hydrogel	516:569	To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method.
31029355	7	58	theme	soft	1303:1306	arg1	engineering					1315:1325	soft tissue engineering	1303:1325	soft tissue engineering	1303:1325	Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
31650251	6	0	theme	typh	835:838	arg1	concentration					840:852	the S. typh concentration	828:852	the S. typh concentration	828:852	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	4	1	theme	solution	672:679	arg1	color					659:663	the color	655:663	the color of the solution	655:679	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
31650251	11	2	theme	Schematic	1181:1189	arg1	representation					1191:1204	Graphical abstract Schematic representation	1162:1204	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.	1162:1355	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	5	3	from	measurement	735:745	arg1	550 nm					768:773	550 nm	768:773	550 nm	768:773	S. typh can be detected via measurement of the absorbance at 550 nm.
31650251	2	4	theme	electrostatic	368:380	arg1	interaction					382:392	electrostatic interaction	368:392	electrostatic interaction to form a composite that acts as the molecular recognition element	368:459	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	9	5	theme	S.	1014:1015	arg1	typh					1017:1020	S. typh	1014:1020	S. typh	1014:1020	The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%.
31650251	10	6	theme	counting	1145:1152	arg1	method					1154:1159	a plate counting method	1137:1159	a plate counting method	1137:1159	The analytical results are basically consistent with those of a plate counting method.
31650251	11	7	theme	assay	1226:1230	arg1	representation					1191:1204	Graphical abstract Schematic representation	1162:1204	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.	1162:1355	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	9	8	theme	typh	1017:1020	arg1	recoveries					1000:1009	The recoveries	996:1009	The recoveries of S. typh from spiked milk samples	996:1045	The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%.
31650251	10	9	theme	plate	1139:1143	arg1	method					1154:1159	a plate counting method	1137:1159	a plate counting method	1137:1159	The analytical results are basically consistent with those of a plate counting method.
31650251	0	10	theme	colorimetric	97:108	arg1	determination					110:122	the colorimetric determination	93:122	the colorimetric determination of Salmonella typhimurium	93:148	A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium.
31650251	10	11	with	consistent	1112:1121	arg1	those					1128:1132	those	1128:1132	those	1128:1132	The analytical results are basically consistent with those of a plate counting method.
31650251	5	12	theme	S.	707:708	arg1	typh					710:713	S. typh	707:713	S. typh	707:713	S. typh can be detected via measurement of the absorbance at 550 nm.
31650251	2	13	theme	molecular	431:439	arg1	element					453:459	the molecular recognition element	427:459	the molecular recognition element	427:459	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	2	13	theme	molecular	431:439	arg1	composite					404:412	a composite	402:412	a composite that acts as the molecular recognition element	402:459	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	9	14	theme	spiked	1027:1032	arg1	samples					1039:1045	spiked milk samples	1027:1045	spiked milk samples	1027:1045	The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%.
31650251	6	15	theme	concentration	840:852	arg1	logarithm					815:823	the logarithm	811:823	the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1	811:890	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	9	16	theme	milk	1034:1037	arg1	samples					1039:1045	spiked milk samples	1027:1045	spiked milk samples	1027:1045	The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%.
31650251	5	17	from	550 nm	768:773	arg1	measurement					735:745	measurement	735:745	measurement of the absorbance at 550 nm	735:773	S. typh can be detected via measurement of the absorbance at 550 nm.
31650251	5	17	from	550 nm	768:773	arg1	absorbance					754:763	the absorbance	750:763	the absorbance at 550 nm	750:773	S. typh can be detected via measurement of the absorbance at 550 nm.
31650251	11	18	theme	colorimetric	1213:1224	arg1	assay					1226:1230	the colorimetric assay	1209:1230	the colorimetric assay	1209:1230	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	6	19	from	logarithm	815:823	arg1	109 cfu·mL-1					879:890	109 cfu·mL-1	879:890	109 cfu·mL-1	879:890	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	6	19	from	logarithm	815:823	arg1	range					861:865	the range	857:865	the range from 100 to 109 cfu·mL-1	857:890	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	11	20	theme	S.	1259:1260	arg1	typhimuium					1247:1256	Salmonella typhimuium	1236:1256	Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites	1236:1354	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	11	20	theme	S.	1259:1260	arg1	typh					1262:1265	S. typh	1259:1265	S. typh	1259:1265	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	8	21	theme	assay	974:978	arg1	specificity					936:946	specificity	936:946	specificity	936:946	The specificity and practicability of the assay were evaluated.
31650251	8	21	theme	assay	974:978	arg1	practicability					952:965	practicability	952:965	practicability	952:965	The specificity and practicability of the assay were evaluated.
31650251	4	22	dep	red	694:696	arg1	blue					701:704	blue	701:704	blue	701:704	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
31650251	4	22	dep	red	694:696	arg1	to					698:699	to	698:699	to	698:699	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
31650251	11	23	theme	abstract	1172:1179	arg1	representation					1191:1204	Graphical abstract Schematic representation	1162:1204	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.	1162:1355	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	7	24	theme	detection	906:914	arg1	limit					897:901	The limit	893:901	The limit of detection	893:914	The limit of detection is 16 cfu·mL-1.
31650251	7	24	theme	detection	906:914	arg1	16 cfu·mL-1					919:929	16 cfu·mL-1	919:929	16 cfu·mL-1	919:929	The limit of detection is 16 cfu·mL-1.
31650251	8	25	dep	specificity	936:946	arg1	The					932:934	The	932:934	The	932:934	The specificity and practicability of the assay were evaluated.
31650251	3	26	theme	S.	481:482	arg1	typh					484:487	S. typh	481:487	S. typh	481:487	In the presence of S. typh, it will be bound by the aptamer, and this changes the structure of the recognition element.
31650251	0	27	theme	typhimurium	138:148	arg1	determination					110:122	the colorimetric determination	93:122	the colorimetric determination of Salmonella typhimurium	93:148	A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium.
31650251	6	28	theme	S.	832:833	arg1	concentration					840:852	the S. typh concentration	828:852	the S. typh concentration	828:852	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	3	29	theme	typh	484:487	arg1	presence					469:476	the presence	465:476	the presence of S. typh	465:487	In the presence of S. typh, it will be bound by the aptamer, and this changes the structure of the recognition element.
31650251	11	30	dep	-gold	1317:1321	arg1	AuNPs					1338:1342	AuNPs	1338:1342	AuNPs	1338:1342	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	11	30	dep	-gold	1317:1321	arg1	nanoparticles					1323:1335	nanoparticles	1323:1335	nanoparticles (AuNPs)	1323:1343	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	6	31	dep	109 cfu·mL-1	879:890	arg1	to					876:877	to	876:877	to	876:877	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	0	32	theme	carboxymethyl	26:38	arg1	chitosan					40:47	carboxymethyl chitosan	26:47	carboxymethyl chitosan	26:47	A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium.
31650251	11	33	theme	Graphical	1162:1170	arg1	representation					1191:1204	Graphical abstract Schematic representation	1162:1204	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.	1162:1355	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	2	34	theme	Carboxymethyl	246:258	arg1	chitosan					260:267	Carboxymethyl chitosan	246:267	Carboxymethyl chitosan	246:267	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	4	35	theme	gold	616:619	arg1	nanoparticles					621:633	the gold nanoparticles	612:633	the gold nanoparticles	612:633	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
31650251	0	36	theme	aptamer-modified	53:68	arg1	nanoparticles					75:87	aptamer-modified gold nanoparticles	53:87	aptamer-modified gold nanoparticles	53:87	A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium.
31650251	3	37	theme	recognition	561:571	arg1	element					573:579	the recognition element	557:579	the recognition element	557:579	In the presence of S. typh, it will be bound by the aptamer, and this changes the structure of the recognition element.
31650251	5	38	theme	absorbance	754:763	arg1	measurement					735:745	measurement	735:745	measurement of the absorbance at 550 nm	735:773	S. typh can be detected via measurement of the absorbance at 550 nm.
31650251	6	39	from	109 cfu·mL-1	879:890	arg1	range					861:865	the range	857:865	the range from 100 to 109 cfu·mL-1	857:890	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	6	39	from	109 cfu·mL-1	879:890	arg1	logarithm					815:823	the logarithm	811:823	the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1	811:890	Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 109 cfu·mL-1.
31650251	3	40	theme	element	573:579	arg1	structure					544:552	the structure	540:552	the structure of the recognition element	540:579	In the presence of S. typh, it will be bound by the aptamer, and this changes the structure of the recognition element.
31650251	10	41	theme	analytical	1079:1088	arg1	results					1090:1096	The analytical results	1075:1096	The analytical results	1075:1096	The analytical results are basically consistent with those of a plate counting method.
31650251	10	41	theme	analytical	1079:1088	arg1	consistent					1112:1121	consistent	1112:1121	consistent	1112:1121	The analytical results are basically consistent with those of a plate counting method.
31650251	2	42	theme	amino-modified	285:298	arg1	aptamer					300:306	amino-modified aptamer	285:306	amino-modified aptamer against S. typh	285:322	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	2	43	theme	gold	346:349	arg1	nanoparticles					351:363	gold nanoparticles	346:363	gold nanoparticles	346:363	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	9	44	from	samples	1039:1045	arg1	recoveries					1000:1009	The recoveries	996:1009	The recoveries of S. typh from spiked milk samples	996:1045	The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%.
31650251	1	45	theme	aptamer-based	154:166	arg1	assay					168:172	An aptamer-based assay	151:172	An aptamer-based assay	151:172	An aptamer-based assay is described for the determination of Salmonella typhimurium (S. typh).
31650251	11	46	theme	-gold	1317:1321	arg1	composites					1345:1354	carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites	1274:1354	carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites	1274:1354	Graphical abstract Schematic representation of the colorimetric assay for Salmonella typhimuium (S. typh) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
31650251	1	47	theme	typhimurium	223:233	arg1	determination					195:207	the determination	191:207	the determination of Salmonella typhimurium (S. typh)	191:243	An aptamer-based assay is described for the determination of Salmonella typhimurium (S. typh).
31650251	1	48	dep	typhimurium	223:233	arg1	typh					239:242	S. typh	236:242	S. typh	236:242	An aptamer-based assay is described for the determination of Salmonella typhimurium (S. typh).
31650251	2	49	theme	recognition	441:451	arg1	element					453:459	the molecular recognition element	427:459	the molecular recognition element	427:459	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	2	49	theme	recognition	441:451	arg1	composite					404:412	a composite	402:412	a composite that acts as the molecular recognition element	402:459	Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh, and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element.
31650251	4	50	theme	solution	602:609	arg1	addition					585:592	addition	585:592	addition of salt solution	585:609	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
31650251	0	51	theme	gold	70:73	arg1	nanoparticles					75:87	aptamer-modified gold nanoparticles	53:87	aptamer-modified gold nanoparticles	53:87	A composite prepared from carboxymethyl chitosan and aptamer-modified gold nanoparticles for the colorimetric determination of Salmonella typhimurium.
31650251	4	52	theme	salt	597:600	arg1	solution					602:609	salt solution	597:609	salt solution	597:609	On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue.
30366306	6	0	theme	LKX	922:924	arg1	high					939:942	high	939:942	high	939:942	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	6	0	theme	LKX	922:924	arg1	alginate					926:933	the LKX alginate	918:933	the LKX alginate	918:933	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	14	1	theme	fibers	2309:2314	arg1	types					2300:2304	the two types	2292:2304	the two types of fibers, namely	2292:2322	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	11	2	theme	GHB	1793:1795	arg1	fibers					1797:1802	the GHB fibers	1789:1802	the GHB fibers	1789:1802	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	6	3	from	high	887:890	arg1	acid					905:908	guluronic acid	895:908	guluronic acid	895:908	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	7	4	theme	calcium	1066:1072	arg1	fibers					1083:1088	calcium alginate fibers	1066:1088	calcium alginate fibers	1066:1088	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	11	5	theme	ion	1924:1926	arg1	content					1928:1934	2.58µmoles/mg calcium ion content	1902:1934	2.58µmoles/mg calcium ion content	1902:1934	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	6	6	theme	guluronic	895:903	arg1	acid					905:908	guluronic acid	895:908	guluronic acid	895:908	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	4	7	theme	wound	661:665	arg1	exudate					667:673	wound exudate	661:673	wound exudate	661:673	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	4	8	used	utilized	534:541	arg2	It					528:529	It	528:529	It	528:529	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	11	9	theme	calcium	1916:1922	arg1	content					1928:1934	2.58µmoles/mg calcium ion content	1902:1934	2.58µmoles/mg calcium ion content	1902:1934	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	14	10	from	break	2282:2286	arg1	modulus					2257:2263	the Young's modulus	2245:2263	the Young's modulus	2245:2263	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	14	10	from	break	2282:2286	arg1	strain					2272:2277	the strain	2268:2277	the strain at break	2268:2286	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	10	11	theme	selected	1703:1710	arg1	properties					1712:1721	selected properties	1703:1721	selected properties of the resultant fibers	1703:1745	This study showed that the alginate type influenced selected properties of the resultant fibers.
30366306	11	12	contain	had	1898:1900	arg1	thicker					1886:1892	thicker	1886:1892	thicker	1886:1892	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	11	12	contain	had	1898:1900	arg1	fibers					1864:1869	the LKX fibers	1856:1869	the LKX fibers	1856:1869	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	11	12	contain	had	1898:1900	arg2	content					1928:1934	2.58µmoles/mg calcium ion content	1902:1934	2.58µmoles/mg calcium ion content	1902:1934	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	7	13	theme	%	1043:1043	arg1	w/v					1045:1047	3% w/v	1042:1047	3% w/v	1042:1047	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	7	13	theme	%	1043:1043	arg1	chloride					1032:1039	calcium chloride	1024:1039	a calcium chloride (3% w/v) bath to produce calcium alginate fibers	1022:1088	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	2	14	theme	cations	277:283	arg1	action					255:260	the action	251:260	the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups	251:392	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	15	15	theme	different	2522:2530	arg1	batches					2532:2538	the different batches	2518:2538	the different batches of fibers	2518:2548	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	5	16	theme	contrasting	840:850	arg1	compositions					852:863	their contrasting compositions	834:863	their contrasting compositions	834:863	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	8	17	theme	72 h.	1152:1156	arg1	properties					1167:1176	72 h. Selected properties	1152:1176	72 h. Selected properties of the blank (unloaded) fibers	1152:1207	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	11	18	theme	mean	1752:1755	arg1	diameter					1757:1764	mean diameter	1752:1764	mean diameter	1752:1764	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	5	19	theme	composition	728:738	arg1	influence					702:710	the potential influence	688:710	the potential influence of the alginate composition on the properties of the resultant fibers	688:780	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	10	20	theme	resultant	1730:1738	arg1	fibers					1740:1745	the resultant fibers	1726:1745	the resultant fibers	1726:1745	This study showed that the alginate type influenced selected properties of the resultant fibers.
30366306	9	21	theme	fibers	1643:1648	arg1	lengths					1632:1638	replicate lengths	1622:1638	replicate lengths of fibers	1622:1648	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	7	22	theme	calcium	1024:1030	arg1	w/v					1045:1047	3% w/v	1042:1047	3% w/v	1042:1047	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	7	22	theme	calcium	1024:1030	arg1	chloride					1032:1039	calcium chloride	1024:1039	a calcium chloride (3% w/v) bath to produce calcium alginate fibers	1022:1088	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	8	23	theme	plasma	1411:1416	arg1	spectroscopy					1434:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	10	24	theme	alginate	1678:1685	arg1	type					1687:1690	the alginate type	1674:1690	the alginate type	1674:1690	This study showed that the alginate type influenced selected properties of the resultant fibers.
30366306	15	25	theme	measured	2378:2385	arg1	reproducible					2497:2508	reproducible	2497:2508	reproducible	2497:2508	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	15	25	theme	measured	2378:2385	arg1	consistent					2415:2424	consistent	2415:2424	consistent	2415:2424	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	15	25	theme	measured	2378:2385	arg1	properties					2387:2396	All the measured properties	2370:2396	All the measured properties	2370:2396	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	1	26	theme	Sodium	135:140	arg1	polysaccharide					174:187	a non-toxic natural polysaccharide	154:187	a non-toxic natural polysaccharide found in marine brown algae	154:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	1	26	theme	Sodium	135:140	arg1	alginate					142:149	Sodium alginate	135:149	Sodium alginate	135:149	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	8	27	theme	emission	1425:1432	arg1	spectroscopy					1434:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	28	theme	optical	1249:1255	arg1	microscopy					1257:1266	optical microscopy	1249:1266	optical microscopy	1249:1266	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	5	29	theme	resultant	765:773	arg1	fibers					775:780	the resultant fibers	761:780	the resultant fibers	761:780	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	7	30	theme	alginate	975:982	arg1	solutions					984:992	The sodium alginate solutions	964:992	The sodium alginate solutions (4% w/w)	964:1001	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	7	30	theme	alginate	975:982	arg1	%					996:996	4% w/w	995:1000	4% w/w	995:1000	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	9	31	theme	aims	1463:1466	arg1	aims					1463:1466	the key aims	1455:1466	the key aims of this work	1455:1479	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	9	31	theme	aims	1463:1466	arg1	One					1448:1450	One	1448:1450	One	1448:1450	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	14	32	theme	Young	2249:2253	arg1	modulus					2257:2263	the Young's modulus	2245:2263	the Young's modulus	2245:2263	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	1	33	theme	non-toxic	156:164	arg1	polysaccharide					174:187	a non-toxic natural polysaccharide	154:187	a non-toxic natural polysaccharide found in marine brown algae	154:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	1	33	theme	non-toxic	156:164	arg1	alginate					142:149	Sodium alginate	135:149	Sodium alginate	135:149	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	12	34	from	differences	2035:2045	arg1	microstructure					2056:2069	their microstructure	2050:2069	their microstructure	2050:2069	The fibers of each alginate could be distinguished visually based on gross differences as well as differences in their microstructure.
30366306	11	35	theme	LKX	1860:1862	arg1	thicker					1886:1892	thicker	1886:1892	thicker	1886:1892	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	11	35	theme	LKX	1860:1862	arg1	fibers					1864:1869	the LKX fibers	1856:1869	the LKX fibers	1856:1869	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	2	36	theme	guluronic	372:380	arg1	groups					387:392	the guluronic acid groups	368:392	the guluronic acid groups	368:392	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	9	37	dep	lengths	1632:1638	arg1	replicate					1622:1630	replicate	1622:1630	replicate	1622:1630	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	8	38	theme	testing	1307:1313	arg1	machine					1315:1321	a universal testing machine	1295:1321	a universal testing machine	1295:1321	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	13	39	theme	non-elastic	2153:2163	arg1	behaviour					2165:2173	largely non-elastic behaviour	2145:2173	largely non-elastic behaviour	2145:2173	Mechanical testing of the fibers produced stress-strain plots displaying largely non-elastic behaviour.
30366306	12	40	from	differences	2012:2022	arg1	microstructure					2056:2069	their microstructure	2050:2069	their microstructure	2050:2069	The fibers of each alginate could be distinguished visually based on gross differences as well as differences in their microstructure.
30366306	12	41	theme	gross	2006:2010	arg1	differences					2012:2022	gross differences	2006:2022	gross differences as well as differences in their microstructure	2006:2069	The fibers of each alginate could be distinguished visually based on gross differences as well as differences in their microstructure.
30366306	12	42	theme	alginate	1956:1963	arg1	fibers					1941:1946	The fibers	1937:1946	The fibers of each alginate	1937:1963	The fibers of each alginate could be distinguished visually based on gross differences as well as differences in their microstructure.
30366306	3	43	theme	many	434:437	arg1	industries					439:448	many industries	434:448	many industries	434:448	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	3	43	theme	many	434:437	arg1	production					466:475	food production	461:475	food production	461:475	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	0	44	theme	physico-chemical	25:40	arg1	properties					42:51	selected physico-chemical properties	16:51	selected physico-chemical properties of calcium alginate fibers	16:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	13	45	theme	stress-strain	2114:2126	arg1	plots					2128:2132	stress-strain plots	2114:2132	stress-strain plots displaying largely non-elastic behaviour	2114:2173	Mechanical testing of the fibers produced stress-strain plots displaying largely non-elastic behaviour.
30366306	5	46	theme	sodium	787:792	arg1	powders					803:809	two sodium alginate powders	783:809	two sodium alginate powders	783:809	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	6	47	theme	GHB	870:872	arg1	high					887:890	high	887:890	high	887:890	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	6	47	theme	GHB	870:872	arg1	alginate					874:881	The GHB alginate	866:881	The GHB alginate	866:881	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	8	48	dep	humidity	1139:1146	arg1	analysed					1214:1221	analysed	1214:1221	were analysed	1209:1221	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	48	dep	humidity	1139:1146	arg1	%					1128:1128	%	1128:1128	%	1128:1128	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	0	49	theme	alginate	64:71	arg1	fibers					73:78	calcium alginate fibers	56:78	calcium alginate fibers	56:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	8	50	theme	electron	1347:1354	arg1	microscopy					1356:1365	scanning electron microscopy	1338:1365	scanning electron microscopy	1338:1365	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	3	51	from	industries	439:448	arg1	popular					423:429	popular	423:429	popular	423:429	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	9	52	theme	large	1550:1554	arg1	lengths					1556:1562	moderately large lengths	1539:1562	moderately large lengths of fiber	1539:1571	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	8	53	dep	%	1128:1128	arg1	32					1126:1127	32	1126:1127	32	1126:1127	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	53	dep	%	1128:1128	arg1	22 °C					1116:1120	22 °C	1116:1120	22 °C	1116:1120	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	3	54	theme	food	461:464	arg1	production					466:475	food production	461:475	food production	461:475	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	8	55	theme	calcium	1372:1378	arg1	content					1380:1386	calcium content	1372:1386	calcium content	1372:1386	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	3	56	theme	biomedical	503:512	arg1	applications					514:525	pharmaceutical and biomedical applications	484:525	pharmaceutical and biomedical applications	484:525	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	13	57	theme	fibers	2098:2103	arg1	testing					2083:2089	Mechanical testing	2072:2089	Mechanical testing of the fibers	2072:2103	Mechanical testing of the fibers produced stress-strain plots displaying largely non-elastic behaviour.
30366306	0	58	theme	calcium	56:62	arg1	fibers					73:78	calcium alginate fibers	56:78	calcium alginate fibers	56:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	4	59	theme	gel	613:615	arg1	capabilities					625:636	gel forming capabilities	613:636	gel forming capabilities upon the absorption of wound exudate	613:673	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	7	60	theme	alginate	1074:1081	arg1	fibers					1083:1088	calcium alginate fibers	1066:1088	calcium alginate fibers	1066:1088	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	3	61	theme	pharmaceutical	484:497	arg1	applications					514:525	pharmaceutical and biomedical applications	484:525	pharmaceutical and biomedical applications	484:525	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	6	62	from	high	939:942	arg1	acid					958:961	mannuronic acid	947:961	mannuronic acid	947:961	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	4	63	theme	products	572:579	arg1	manufacture					546:556	manufacture	546:556	manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate	546:673	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	2	64	theme	poly-valent	265:275	arg1	cations					277:283	poly-valent cations	265:283	poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups	265:392	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	13	65	theme	Mechanical	2072:2081	arg1	testing					2083:2089	Mechanical testing	2072:2089	Mechanical testing of the fibers	2072:2103	Mechanical testing of the fibers produced stress-strain plots displaying largely non-elastic behaviour.
30366306	15	66	theme	sampling	2441:2448	arg1	positions					2450:2458	the nine sampling positions	2432:2458	the nine sampling positions along the lengths of fibers	2432:2486	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	6	67	from	acid	905:908	arg1	high					887:890	high	887:890	high	887:890	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	6	67	from	acid	905:908	arg1	alginate					874:881	The GHB alginate	866:881	The GHB alginate	866:881	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	3	68	from	applications	514:525	arg1	popular					423:429	popular	423:429	popular	423:429	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	11	69	theme	2.58µmoles/mg	1902:1914	arg1	content					1928:1934	2.58µmoles/mg calcium ion content	1902:1934	2.58µmoles/mg calcium ion content	1902:1934	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	1	70	theme	natural	166:172	arg1	polysaccharide					174:187	a non-toxic natural polysaccharide	154:187	a non-toxic natural polysaccharide found in marine brown algae	154:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	1	70	theme	natural	166:172	arg1	alginate					142:149	Sodium alginate	135:149	Sodium alginate	135:149	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	14	71	dep	significant	2203:2213	arg1	p < 0.05					2216:2223	p < 0.05	2216:2223	p < 0.05	2216:2223	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	4	72	theme	exudate	667:673	arg1	absorption					647:656	the absorption	643:656	the absorption of wound exudate	643:673	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	0	73	theme	alginate	125:132	arg1	types					109:113	two different types	95:113	two different types of sodium alginate	95:132	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	15	74	theme	fibers	2543:2548	arg1	batches					2532:2538	the different batches	2518:2538	the different batches of fibers	2518:2548	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
30366306	1	75	theme	marine	198:203	arg1	algae					211:215	marine brown algae	198:215	marine brown algae	198:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	8	76	theme	relative	1130:1137	arg1	humidity					1139:1146	22 °C and 32% relative humidity	1116:1146	22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed	1116:1221	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	77	theme	unloaded	1192:1199	arg1	fibers					1202:1207	the blank (unloaded) fibers	1181:1207	the blank (unloaded) fibers	1181:1207	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	7	78	theme	chloride	1032:1039	arg1	bath					1050:1053	a calcium chloride (3% w/v) bath	1022:1053	a calcium chloride (3% w/v) bath to produce calcium alginate fibers	1022:1088	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	10	79	theme	fibers	1740:1745	arg1	properties					1712:1721	selected properties	1703:1721	selected properties of the resultant fibers	1703:1745	This study showed that the alginate type influenced selected properties of the resultant fibers.
30366306	8	80	theme	blank	1185:1189	arg1	fibers					1202:1207	the blank (unloaded) fibers	1181:1207	the blank (unloaded) fibers	1181:1207	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	6	81	theme	mannuronic	947:956	arg1	acid					958:961	mannuronic acid	947:961	mannuronic acid	947:961	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	8	82	theme	coupled	1403:1409	arg1	spectroscopy					1434:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	83	theme	fibers	1202:1207	arg1	properties					1167:1176	72 h. Selected properties	1152:1176	72 h. Selected properties of the blank (unloaded) fibers	1152:1207	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	1	84	located	found	189:193	arg1	algae					211:215	marine brown algae	198:215	marine brown algae	198:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	1	84	located	found	189:193	arg2	alginate					142:149	Sodium alginate	135:149	Sodium alginate	135:149	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	1	84	located	found	189:193	arg2	polysaccharide					174:187	a non-toxic natural polysaccharide	154:187	a non-toxic natural polysaccharide found in marine brown algae	154:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	8	85	theme	Selected	1158:1165	arg1	properties					1167:1176	72 h. Selected properties	1152:1176	72 h. Selected properties of the blank (unloaded) fibers	1152:1207	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	86	theme	atomic	1418:1423	arg1	spectroscopy					1434:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	inductively coupled plasma atomic emission spectroscopy	1391:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	2	87	dep	cations	277:283	arg1	magnesium					311:319	magnesium	311:319	commonly calcium but not magnesium	286:319	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	2	87	dep	cations	277:283	arg1	calcium					295:301	calcium	295:301	commonly calcium but not magnesium	286:319	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	5	88	theme	potential	692:700	arg1	influence					702:710	the potential influence	688:710	the potential influence of the alginate composition on the properties of the resultant fibers	688:780	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	7	89	theme	w/w	998:1000	arg1	solutions					984:992	The sodium alginate solutions	964:992	The sodium alginate solutions (4% w/w)	964:1001	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	7	89	theme	w/w	998:1000	arg1	%					996:996	4% w/w	995:1000	4% w/w	995:1000	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	8	90	theme	diameter	1224:1231	arg1	measurements					1233:1244	diameter measurements	1224:1244	diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy	1224:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	0	91	theme	fibers	73:78	arg1	properties					42:51	selected physico-chemical properties	16:51	selected physico-chemical properties of calcium alginate fibers	16:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	2	92	theme	solid	237:241	arg1	gels					243:246	solid gels	237:246	solid gels	237:246	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	9	93	theme	key	1459:1461	arg1	aims					1463:1466	the key aims	1455:1466	the key aims of this work	1455:1479	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	5	94	theme	fibers	775:780	arg1	properties					747:756	the properties	743:756	the properties of the resultant fibers	743:780	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	0	95	theme	sodium	118:123	arg1	alginate					125:132	sodium alginate	118:132	sodium alginate	118:132	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	0	96	theme	different	99:107	arg1	types					109:113	two different types	95:113	two different types of sodium alginate	95:132	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	7	97	theme	sodium	968:973	arg1	solutions					984:992	The sodium alginate solutions	964:992	The sodium alginate solutions (4% w/w)	964:1001	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	7	97	theme	sodium	968:973	arg1	%					996:996	4% w/w	995:1000	4% w/w	995:1000	The sodium alginate solutions (4% w/w) were extruded into a calcium chloride (3% w/v) bath to produce calcium alginate fibers.
30366306	9	98	theme	work	1476:1479	arg1	aims					1463:1466	the key aims	1455:1466	the key aims of this work	1455:1479	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	8	99	dep	dried	1107:1111	arg1	measurements					1233:1244	diameter measurements	1224:1244	diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy	1224:1445	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	8	100	theme	mechanical	1269:1278	arg1	strength					1280:1287	mechanical strength	1269:1287	mechanical strength using a universal testing machine	1269:1321	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	1	101	theme	brown	205:209	arg1	algae					211:215	marine brown algae	198:215	marine brown algae	198:215	Sodium alginate is a non-toxic natural polysaccharide found in marine brown algae.
30366306	11	102	dep	diameter	1757:1764	arg1	The					1748:1750	The	1748:1750	The	1748:1750	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	5	103	theme	alginate	719:726	arg1	composition					728:738	the alginate composition	715:738	the alginate composition	715:738	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	8	104	theme	universal	1297:1305	arg1	machine					1315:1321	a universal testing machine	1295:1321	a universal testing machine	1295:1321	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	14	105	theme	significant	2203:2213	arg1	difference					2226:2235	no statistically significant (p < 0.05) difference	2186:2235	no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely	2186:2322	There was no statistically significant (p < 0.05) difference between the Young's modulus or the strain at break for the two types of fibers, namely about 5-5.6 GPa and 0.123-0.131 respectively.
30366306	0	106	theme	selected	16:23	arg1	properties					42:51	selected physico-chemical properties	16:51	selected physico-chemical properties of calcium alginate fibers	16:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	4	107	theme	wound-care	561:570	arg1	products					572:579	wound-care products	561:579	wound-care products	561:579	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	6	108	from	acid	958:961	arg1	high					939:942	high	939:942	high	939:942	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	6	108	from	acid	958:961	arg1	alginate					926:933	the LKX alginate	918:933	the LKX alginate	918:933	The GHB alginate was high in guluronic acid whereas the LKX alginate was high in mannuronic acid.
30366306	2	109	theme	polysaccharide	343:356	arg1	chains					358:363	the polysaccharide chains	339:363	the polysaccharide chains	339:363	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	0	110	theme	properties	42:51	arg1	comparison					2:11	A comparison	0:11	A comparison of selected physico-chemical properties of calcium alginate fibers	0:78	A comparison of selected physico-chemical properties of calcium alginate fibers produced using two different types of sodium alginate.
30366306	9	111	theme	properties	1522:1531	arg1	variability					1501:1511	the variability	1497:1511	the variability of these properties along moderately large lengths of fiber	1497:1571	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	11	112	theme	fibers	1797:1802	arg1	content					1778:1784	calcium content	1770:1784	calcium content	1770:1784	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	11	112	theme	fibers	1797:1802	arg1	diameter					1757:1764	mean diameter	1752:1764	mean diameter	1752:1764	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	3	113	theme	Alginate-based	395:408	arg1	products					410:417	Alginate-based products	395:417	Alginate-based products	395:417	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	2	114	theme	acid	382:385	arg1	groups					387:392	the guluronic acid groups	368:392	the guluronic acid groups	368:392	It is able to form solid gels by the action of poly-valent cations (commonly calcium but not magnesium) which cross-link the polysaccharide chains at the guluronic acid groups.
30366306	5	115	theme	alginate	794:801	arg1	powders					803:809	two sodium alginate powders	783:809	two sodium alginate powders	783:809	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	8	116	theme	scanning	1338:1345	arg1	microscopy					1356:1365	scanning electron microscopy	1338:1365	scanning electron microscopy	1338:1365	The fibers were dried at 22 °C and 32% relative humidity for 72 h. Selected properties of the blank (unloaded) fibers were analysed: diameter measurements by optical microscopy, mechanical strength using a universal testing machine, morphology by scanning electron microscopy, and calcium content by inductively coupled plasma atomic emission spectroscopy.
30366306	11	117	theme	calcium	1770:1776	arg1	content					1778:1784	calcium content	1770:1784	calcium content	1770:1784	The mean diameter and calcium content of the GHB fibers were 232 µm and 2.79µmoles/mg respectively, whereas the LKX fibers were about 10% thicker and had 2.58µmoles/mg calcium ion content.
30366306	5	118	from	influence	702:710	arg1	properties					747:756	the properties	743:756	the properties of the resultant fibers	743:780	Considering the potential influence of the alginate composition on the properties of the resultant fibers, two sodium alginate powders were selected based on their contrasting compositions.
30366306	9	119	theme	fiber	1567:1571	arg1	lengths					1556:1562	moderately large lengths	1539:1562	moderately large lengths of fiber	1539:1571	One of the key aims of this work was to evaluate the variability of these properties along moderately large lengths of fiber and to determine the difference (if any) between replicate lengths of fibers.
30366306	4	120	theme	forming	617:623	arg1	capabilities					625:636	gel forming capabilities	613:636	gel forming capabilities upon the absorption of wound exudate	613:673	It is utilized in manufacture of wound-care products due to its biocompatibility and gel forming capabilities upon the absorption of wound exudate.
30366306	3	121	from	popular	423:429	arg1	industries					439:448	many industries	434:448	many industries	434:448	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	3	121	from	popular	423:429	arg1	production					466:475	food production	461:475	food production	461:475	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	3	121	from	popular	423:429	arg1	applications					514:525	pharmaceutical and biomedical applications	484:525	pharmaceutical and biomedical applications	484:525	Alginate-based products are popular in many industries, including food production and in pharmaceutical and biomedical applications.
30366306	15	122	theme	fibers	2481:2486	arg1	lengths					2470:2476	the lengths	2466:2476	the lengths of fibers	2466:2486	All the measured properties were found to be consistent along the nine sampling positions along the lengths of fibers and were reproducible between the different batches of fibers.
29330852	4	0	theme	galacturonic	1005:1016	arg1	acid					1018:1021	galacturonic acid	1005:1021	galacturonic acid	1005:1021	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	5	1	theme	acids	1177:1181	arg1	content					1139:1145	the content	1135:1145	the content of soluble conjugate phenolic acids	1135:1181	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	5	2	theme	storage	1109:1115	arg1	period					1117:1122	storage period	1109:1122	storage period of Chenpi	1109:1132	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	6	3	theme	bone	1379:1382	arg1	cells					1391:1395	bone marrow cells	1379:1395	bone marrow cells	1379:1395	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	6	4	theme	10-year	1315:1321	arg1	Chenpi					1323:1328	the 5-year and 10-year Chenpi	1300:1328	the 5-year and 10-year Chenpi	1300:1328	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	4	5	theme	rhamnose	947:954	arg1	amount					937:942	a small amount	929:942	a small amount of rhamnose	929:954	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	5	theme	rhamnose	947:954	arg1	galactose					914:922	galactose	914:922	galactose	914:922	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	5	theme	rhamnose	947:954	arg1	arabinose					881:889	arabinose	881:889	arabinose	881:889	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	5	theme	rhamnose	947:954	arg1	acid					905:908	galacturonic acid	892:908	galacturonic acid	892:908	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	5	theme	rhamnose	947:954	arg1	rhamnose					947:954	rhamnose	947:954	rhamnose	947:954	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	5	6	theme	Chenpi	1127:1132	arg1	period					1117:1122	storage period	1109:1122	storage period of Chenpi	1109:1132	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	7	7	from	differences	1492:1502	arg1	polysaccharides					1518:1532	the pectic polysaccharides	1507:1532	the pectic polysaccharides from Chenpi with different storage periods	1507:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	7	from	differences	1492:1502	arg1	Chenpi					1539:1544	Chenpi	1539:1544	Chenpi with different storage periods	1539:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	4	8	theme	composition	824:834	arg1	analysis					836:843	Monosaccharide composition analysis	809:843	Monosaccharide composition analysis	809:843	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	6	9	theme	cells	1391:1395	arg1	proliferation					1362:1374	the proliferation	1358:1374	the proliferation of bone marrow cells	1358:1395	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	0	10	theme	storage	126:132	arg1	periods					134:140	different storage periods	116:140	different storage periods	116:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	0	11	from	Characteristics	0:14	arg1	Chenpi					104:109	Chenpi	104:109	Chenpi with different storage periods	104:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	0	12	with	Chenpi	104:109	arg1	periods					134:140	different storage periods	116:140	different storage periods	116:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	4	13	theme	small	1061:1065	arg1	rhamnose					1077:1084	rhamnose	1077:1084	rhamnose	1077:1084	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	13	theme	small	1061:1065	arg1	amount					1067:1072	a small amount	1059:1072	a small amount of rhamnose	1059:1084	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	1	14	theme	fresh	284:288	arg1	Chenpi					290:295	fresh Chenpi	284:295	fresh Chenpi	284:295	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	0	15	theme	different	116:124	arg1	periods					134:140	different storage periods	116:140	different storage periods	116:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	7	16	theme	present	1461:1467	arg1	study					1469:1473	The present study	1457:1473	The present study	1457:1473	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	6	17	theme	pectic	1262:1267	arg1	polysaccharides					1269:1283	the pectic polysaccharides	1258:1283	the pectic polysaccharides extracted from the 5-year and 10-year Chenpi	1258:1328	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	8	18	theme	healthcare	1856:1865	arg1	effects					1867:1873	better healthcare effects	1849:1873	better healthcare effects	1849:1873	This phenomenon might partly explain why the Chenpi stored over the long-term has better healthcare effects.
29330852	8	19	contain	has	1845:1847	arg2	effects					1867:1873	better healthcare effects	1849:1873	better healthcare effects	1849:1873	This phenomenon might partly explain why the Chenpi stored over the long-term has better healthcare effects.
29330852	8	19	contain	has	1845:1847	arg1	Chenpi					1812:1817	the Chenpi	1808:1817	the Chenpi stored over the long-term	1808:1843	This phenomenon might partly explain why the Chenpi stored over the long-term has better healthcare effects.
29330852	3	20	theme	higher	759:764	arg1	heterogeneity					776:788	a higher molecular heterogeneity	757:788	a higher molecular heterogeneity	757:788	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	8	21	theme	better	1849:1854	arg1	effects					1867:1873	better healthcare effects	1849:1873	better healthcare effects	1849:1873	This phenomenon might partly explain why the Chenpi stored over the long-term has better healthcare effects.
29330852	1	22	dep	BACKGROUND	143:152	arg1	considers					175:183	considers	175:183	considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure	175:365	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	1	23	theme	dried	198:202	arg1	peel					211:214	dried citrus peel	198:214	dried citrus peel	198:214	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	1	23	theme	dried	198:202	arg1	Chenpi					190:195	Chenpi	190:195	Chenpi (dried citrus peel) stored over the long-term	190:241	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	3	24	gly	heterogeneity	776:788	arg1	methylesterification					724:743	methylesterification	724:743	methylesterification	724:743	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	6	25	theme	patch	1424:1428	arg1	cells					1430:1434	the Peyer's patch cells	1412:1434	the Peyer's patch cells	1412:1434	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	1	26	theme	citrus	204:209	arg1	peel					211:214	dried citrus peel	198:214	dried citrus peel	198:214	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	1	26	theme	citrus	204:209	arg1	Chenpi					190:195	Chenpi	190:195	Chenpi (dried citrus peel) stored over the long-term	190:241	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	0	27	from	activities	48:57	arg1	Chenpi					104:109	Chenpi	104:109	Chenpi with different storage periods	104:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	3	28	theme	methylesterification	724:743	arg1	viscosity					700:708	a lower dynamic viscosity	684:708	a lower dynamic viscosity	684:708	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	3	28	theme	methylesterification	724:743	arg1	heterogeneity					776:788	a higher molecular heterogeneity	757:788	a higher molecular heterogeneity	757:788	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	3	28	theme	methylesterification	724:743	arg1	degree					714:719	degree	714:719	degree of methylesterification	714:743	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	7	29	with	Chenpi	1539:1544	arg1	periods					1569:1575	different storage periods	1551:1575	different storage periods	1551:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	5	30	theme	conjugate	1158:1166	arg1	acids					1177:1181	soluble conjugate phenolic acids	1150:1181	soluble conjugate phenolic acids	1150:1181	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	4	31	theme	galacturonic	892:903	arg1	acid					905:908	galacturonic acid	892:908	galacturonic acid	892:908	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	7	32	theme	storage	1561:1567	arg1	periods					1569:1575	different storage periods	1551:1575	different storage periods	1551:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	33	theme	intestinal	1696:1705	arg1	activities					1707:1716	more significant intestinal activities	1679:1716	more significant intestinal activities	1679:1716	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	3	34	theme	lower	686:690	arg1	viscosity					700:708	a lower dynamic viscosity	684:708	a lower dynamic viscosity	684:708	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	0	35	theme	immunomodulating	31:46	arg1	activities					48:57	intestinal immunomodulating activities	20:57	intestinal immunomodulating activities	20:57	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	5	36	theme	phenolic	1168:1175	arg1	acids					1177:1181	soluble conjugate phenolic acids	1150:1181	soluble conjugate phenolic acids	1150:1181	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	2	37	dep	RESULTS	368:374	arg1	investigated					596:607	investigated	596:607	investigated	596:607	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	37	dep	RESULTS	368:374	arg1	obtained					451:458	obtained	451:458	were obtained from 1-, 5- and 10-year Chenpi, respectively	446:503	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	0	38	theme	intestinal	20:29	arg1	activities					48:57	intestinal immunomodulating activities	20:57	intestinal immunomodulating activities	20:57	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	3	39	theme	molecular	766:774	arg1	heterogeneity					776:788	a higher molecular heterogeneity	757:788	a higher molecular heterogeneity	757:788	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	2	40	theme	immunomodulating	563:578	arg1	activities					580:589	intestinal immunomodulating activities	552:589	intestinal immunomodulating activities	552:589	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	7	41	theme	different	1551:1559	arg1	periods					1569:1575	different storage periods	1551:1575	different storage periods	1551:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	4	42	dep	composed	869:876	arg1	consisted					981:989	consisted	981:989	consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose	981:1084	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	2	43	theme	10-year	476:482	arg1	Chenpi					484:489	10-year Chenpi	476:489	10-year Chenpi	476:489	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	1	44	theme	detailed	311:318	arg1	mechanism					320:328	the detailed mechanism	307:328	the detailed mechanism responsible for this	307:349	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	2	45	theme	intestinal	552:561	arg1	activities					580:589	intestinal immunomodulating activities	552:589	intestinal immunomodulating activities	552:589	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	0	46	from	Chenpi	104:109	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics	0:14	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	0	46	from	Chenpi	104:109	arg1	polysaccharides					83:97	water-soluble pectic polysaccharides	62:97	water-soluble pectic polysaccharides from Chenpi with different storage periods	62:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	0	46	from	Chenpi	104:109	arg1	activities					48:57	intestinal immunomodulating activities	20:57	intestinal immunomodulating activities	20:57	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	7	47	theme	significant	1684:1694	arg1	activities					1707:1716	more significant intestinal activities	1679:1716	more significant intestinal activities	1679:1716	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	48	from	Chenpi	1539:1544	arg1	polysaccharides					1518:1532	the pectic polysaccharides	1507:1532	the pectic polysaccharides from Chenpi with different storage periods	1507:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	48	from	Chenpi	1539:1544	arg1	differences					1492:1502	the differences	1488:1502	the differences in the pectic polysaccharides from Chenpi with different storage periods	1488:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	49	dep	CONCLUSION	1446:1455	arg1	confirms					1586:1593	confirms	1586:1593	confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi	1586:1764	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	49	dep	CONCLUSION	1446:1455	arg1	demonstrates					1475:1486	demonstrates	1475:1486	demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods	1475:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	0	50	theme	pectic	76:81	arg1	polysaccharides					83:97	water-soluble pectic polysaccharides	62:97	water-soluble pectic polysaccharides from Chenpi with different storage periods	62:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	1	51	theme	responsible	330:340	arg1	mechanism					320:328	the detailed mechanism	307:328	the detailed mechanism responsible for this	307:349	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	4	52	theme	small	931:935	arg1	amount					937:942	a small amount	929:942	a small amount of rhamnose	929:954	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	52	theme	small	931:935	arg1	rhamnose					947:954	rhamnose	947:954	rhamnose	947:954	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	0	53	theme	water-soluble	62:74	arg1	polysaccharides					83:97	water-soluble pectic polysaccharides	62:97	water-soluble pectic polysaccharides from Chenpi with different storage periods	62:140	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	1	54	contain	has	243:245	arg1	peel					211:214	dried citrus peel	198:214	dried citrus peel	198:214	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	1	54	contain	has	243:245	arg2	efficacies					261:270	better health efficacies	247:270	better health efficacies	247:270	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	1	54	contain	has	243:245	arg1	Chenpi					190:195	Chenpi	190:195	Chenpi (dried citrus peel) stored over the long-term	190:241	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	4	55	theme	rhamnose	1077:1084	arg1	galactose					1024:1032	galactose	1024:1032	galactose	1024:1032	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	arabinose					994:1002	arabinose	994:1002	arabinose	994:1002	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	amount					1067:1072	a small amount	1059:1072	a small amount of rhamnose	1059:1084	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	glucose					1035:1041	glucose	1035:1041	glucose	1035:1041	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	xylose					1047:1052	xylose	1047:1052	xylose	1047:1052	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	rhamnose					1077:1084	rhamnose	1077:1084	rhamnose	1077:1084	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	4	55	theme	rhamnose	1077:1084	arg1	acid					1018:1021	galacturonic acid	1005:1021	galacturonic acid	1005:1021	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	5	56	theme	soluble	1150:1156	arg1	acids					1177:1181	soluble conjugate phenolic acids	1150:1181	soluble conjugate phenolic acids	1150:1181	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	6	57	theme	5-year	1304:1309	arg1	Chenpi					1323:1328	the 5-year and 10-year Chenpi	1300:1328	the 5-year and 10-year Chenpi	1300:1328	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	5	58	theme	pectic	1200:1205	arg1	polysaccharide					1207:1220	the pectic polysaccharide	1196:1220	the pectic polysaccharide	1196:1220	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	1	59	theme	traditional	158:168	arg1	view					170:173	The traditional view	154:173	The traditional view	154:173	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	7	60	contain	had	1675:1677	arg2	activities					1707:1716	more significant intestinal activities	1679:1716	more significant intestinal activities	1679:1716	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	60	contain	had	1675:1677	arg1	polysaccharides					1611:1625	the pectic polysaccharides	1600:1625	the pectic polysaccharides extracted from Chenpi stored over the long-term	1600:1673	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	7	61	theme	pectic	1604:1609	arg1	polysaccharides					1611:1625	the pectic polysaccharides	1600:1625	the pectic polysaccharides extracted from Chenpi stored over the long-term	1600:1673	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	4	62	theme	Monosaccharide	809:822	arg1	analysis					836:843	Monosaccharide composition analysis	809:843	Monosaccharide composition analysis	809:843	Monosaccharide composition analysis indicated that CPP1 was composed of arabinose, galacturonic acid and galactose, and a small amount of rhamnose; however, CPP5 and CPP10 consisted of arabinose, galacturonic acid, galactose, glucose and xylose, and a small amount of rhamnose.
29330852	3	63	theme	dynamic	692:698	arg1	viscosity					700:708	a lower dynamic viscosity	684:708	a lower dynamic viscosity	684:708	The results obtained showed that CPP5 and CPP10 demonstrated a lower dynamic viscosity and degree of methylesterification, as well as a higher molecular heterogeneity, compared to CPP1.
29330852	2	64	dep	polysaccharides	407:421	arg1	CPP1					424:427	CPP1	424:427	CPP1	424:427	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	64	dep	polysaccharides	407:421	arg1	polysaccharides					407:421	The three water-soluble pectic polysaccharides	376:421	The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10)	376:444	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	64	dep	polysaccharides	407:421	arg1	CPP10					439:443	CPP10	439:443	CPP10	439:443	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	64	dep	polysaccharides	407:421	arg1	CPP5					430:433	CPP5	430:433	CPP5	430:433	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	9	65	theme	Chemical	1894:1901	arg1	Industry					1903:1910	Chemical Industry	1894:1910	Chemical Industry	1894:1910	© 2018 Society of Chemical Industry.
29330852	1	66	theme	better	247:252	arg1	efficacies					261:270	better health efficacies	247:270	better health efficacies	247:270	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	7	67	theme	pectic	1511:1516	arg1	polysaccharides					1518:1532	the pectic polysaccharides	1507:1532	the pectic polysaccharides from Chenpi with different storage periods	1507:1575	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	6	68	theme	marrow	1384:1389	arg1	cells					1391:1395	bone marrow cells	1379:1395	bone marrow cells	1379:1395	Furthermore, it was confirmed that the pectic polysaccharides extracted from the 5-year and 10-year Chenpi could significantly enhance the proliferation of bone marrow cells via activating the Peyer's patch cells in vitro.
29330852	2	69	theme	pectic	400:405	arg1	CPP1					424:427	CPP1	424:427	CPP1	424:427	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	69	theme	pectic	400:405	arg1	polysaccharides					407:421	The three water-soluble pectic polysaccharides	376:421	The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10)	376:444	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	69	theme	pectic	400:405	arg1	CPP10					439:443	CPP10	439:443	CPP10	439:443	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	69	theme	pectic	400:405	arg1	CPP5					430:433	CPP5	430:433	CPP5	430:433	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	1	70	theme	health	254:259	arg1	efficacies					261:270	better health efficacies	247:270	better health efficacies	247:270	BACKGROUND The traditional view considers that Chenpi (dried citrus peel) stored over the long-term has better health efficacies compared to fresh Chenpi, although the detailed mechanism responsible for this remains obscure.
29330852	0	71	theme	polysaccharides	83:97	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics	0:14	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	0	71	theme	polysaccharides	83:97	arg1	activities					48:57	intestinal immunomodulating activities	20:57	intestinal immunomodulating activities	20:57	Characteristics and intestinal immunomodulating activities of water-soluble pectic polysaccharides from Chenpi with different storage periods.
29330852	5	72	theme	period	1117:1122	arg1	extension					1096:1104	the extension	1092:1104	the extension of storage period of Chenpi	1092:1132	With the extension of storage period of Chenpi, the content of soluble conjugate phenolic acids increased in the pectic polysaccharide.
29330852	2	73	theme	water-soluble	386:398	arg1	CPP1					424:427	CPP1	424:427	CPP1	424:427	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	73	theme	water-soluble	386:398	arg1	polysaccharides					407:421	The three water-soluble pectic polysaccharides	376:421	The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10)	376:444	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	73	theme	water-soluble	386:398	arg1	CPP10					439:443	CPP10	439:443	CPP10	439:443	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	2	73	theme	water-soluble	386:398	arg1	CPP5					430:433	CPP5	430:433	CPP5	430:433	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29330852	7	74	theme	fresh	1753:1757	arg1	Chenpi					1759:1764	the fresh Chenpi	1749:1764	the fresh Chenpi	1749:1764	CONCLUSION The present study demonstrates the differences in the pectic polysaccharides from Chenpi with different storage periods and also confirms that the pectic polysaccharides extracted from Chenpi stored over the long-term had more significant intestinal activities compared to that obtained from the fresh Chenpi.
29330852	2	75	theme	physicochemical	516:530	arg1	characteristics					532:546	their physicochemical characteristics	510:546	their physicochemical characteristics	510:546	RESULTS The three water-soluble pectic polysaccharides (CPP1, CPP5 and CPP10) were obtained from 1-, 5- and 10-year Chenpi, respectively, and their physicochemical characteristics and intestinal immunomodulating activities were investigated and compared.
29520710	9	0	theme	binding	1508:1514	arg1	modes					1516:1520	its binding modes	1504:1520	its binding modes with HS	1504:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	6	1	theme	Ig2	1009:1011	arg1	domains					1013:1019	the Ig1 and Ig2 domains	997:1019	the Ig1 and Ig2 domains of Robo1	997:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	1	theme	Ig2	1009:1011	arg1	Robo1					1024:1028	Robo1	1024:1028	Robo1	1024:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	2	theme	hinge	973:977	arg1	region					979:984	the highly flexible interdomain hinge region	941:984	the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1	941:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	8	3	theme	HS	1424:1425	arg1	modification					1408:1419	modification	1408:1419	modification of HS	1408:1425	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	8	4	theme	pattern	1397:1403	arg1	presence					1365:1372	the presence	1361:1372	the presence of a specific size and pattern of modification of HS	1361:1425	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	9	5	from	effect	1445:1450	arg1	conformation					1478:1489	the conformation	1474:1489	the conformation of Robo1	1474:1498	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	9	5	from	effect	1445:1450	arg1	modes					1516:1520	its binding modes	1504:1520	its binding modes with HS	1504:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	0	6	theme	Sulfate	78:84	arg1	Interaction					86:96	the Robo1-Heparan Sulfate Interaction	60:96	the Robo1-Heparan Sulfate Interaction	60:96	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	1	7	theme	nonneuronal	305:315	arg1	tissues					317:323	the nonneuronal tissues	301:323	the nonneuronal tissues	301:323	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	8	from	morphogenesis	260:272	arg1	tissues					317:323	the nonneuronal tissues	301:323	the nonneuronal tissues	301:323	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	3	9	theme	signaling	509:517	arg1	complex					519:525	the minimal signaling complex	497:525	the minimal signaling complex	497:525	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	4	10	theme	ion	684:686	arg1	TWIMS					711:715	TWIMS	711:715	TWIMS	711:715	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	4	10	theme	ion	684:686	arg1	spectrometry					697:708	wave ion mobility spectrometry	679:708	wave ion mobility spectrometry (TWIMS)	679:716	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	9	11	theme	Robo1	1494:1498	arg1	conformation					1478:1489	the conformation	1474:1489	the conformation of Robo1	1474:1498	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	9	11	theme	Robo1	1494:1498	arg1	modes					1516:1520	its binding modes	1504:1520	its binding modes with HS	1504:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	0	12	theme	Robo1-Heparan	64:76	arg1	Interaction					86:96	the Robo1-Heparan Sulfate Interaction	60:96	the Robo1-Heparan Sulfate Interaction	60:96	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	4	13	theme	wave	679:682	arg1	TWIMS					711:715	TWIMS	711:715	TWIMS	711:715	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	4	13	theme	wave	679:682	arg1	spectrometry					697:708	wave ion mobility spectrometry	679:708	wave ion mobility spectrometry (TWIMS)	679:716	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	1	14	theme	nervous	229:235	arg1	system					237:242	the nervous system	225:242	the nervous system	225:242	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	6	15	theme	important	1039:1047	arg1	role					1060:1063	an important functional role	1036:1063	an important functional role	1036:1063	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	16	theme	Robo1-Slit	1082:1091	arg1	interaction					1093:1103	the Robo1-Slit interaction	1078:1103	the Robo1-Slit interaction	1078:1103	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	17	theme	Ig1	1001:1003	arg1	domains					1013:1019	the Ig1 and Ig2 domains	997:1019	the Ig1 and Ig2 domains of Robo1	997:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	17	theme	Ig1	1001:1003	arg1	Robo1					1024:1028	Robo1	1024:1028	Robo1	1024:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	0	18	theme	Interaction	86:96	arg1	Study					51:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study	0:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	3	19	theme	Robo1	475:479	arg1	binding					464:470	the binding	460:470	the binding of Robo1 to Slit	460:487	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	8	20	theme	specific	1379:1386	arg1	size					1388:1391	a specific size	1377:1391	a specific size	1377:1391	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	7	21	theme	different	1242:1250	arg1	conformations					1252:1264	different conformations	1242:1264	different conformations of Robo1	1242:1273	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	0	22	theme	Traveling	2:10	arg1	Study					51:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study	0:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	7	23	theme	sulfation	1134:1142	arg1	pattern					1144:1150	sulfation pattern	1134:1150	sulfation pattern	1134:1150	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	4	24	theme	interaction	770:780	arg1	details					746:752	the conformational details	727:752	the conformational details of the Robo1-HS interaction	727:780	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	8	25	theme	MS	1281:1282	arg1	measurements					1284:1295	MS measurements	1281:1295	MS measurements	1281:1295	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	10	26	theme	Graphical	1543:1551	arg1	Abstract					1553:1560	Graphical Abstract	1543:1560	Graphical Abstract	1543:1560	Graphical Abstract ᅟ.
29520710	7	27	dep	pattern	1144:1150	arg1	the					1130:1132	the	1130:1132	the	1130:1132	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	4	28	theme	Robo1-HS	761:768	arg1	interaction					770:780	the Robo1-HS interaction	757:780	the Robo1-HS interaction	757:780	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	9	29	with	modes	1516:1520	arg1	HS					1527:1528	HS	1527:1528	HS	1527:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	3	30	theme	minimal	501:507	arg1	complex					519:525	the minimal signaling complex	497:525	the minimal signaling complex	497:525	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	0	31	theme	Ion	17:19	arg1	Spectrometry					30:41	Wave Ion Mobility Spectrometry	12:41	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	0	31	theme	Ion	17:19	arg1	TWIMS					44:48	TWIMS	44:48	TWIMS	44:48	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	1	32	theme	axon	165:168	arg1	guidance					170:177	axon guidance	165:177	axon guidance	165:177	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	0	33	theme	Wave	12:15	arg1	Spectrometry					30:41	Wave Ion Mobility Spectrometry	12:41	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	0	33	theme	Wave	12:15	arg1	TWIMS					44:48	TWIMS	44:48	TWIMS	44:48	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	9	34	theme	N-glycosylation	1455:1469	arg1	effect					1445:1450	the effect	1441:1450	the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS	1441:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	6	35	theme	functional	1049:1058	arg1	role					1060:1063	an important functional role	1036:1063	an important functional role	1036:1063	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	36	theme	interdomain	961:971	arg1	region					979:984	the highly flexible interdomain hinge region	941:984	the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1	941:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	0	37	theme	Spectrometry	30:41	arg1	Study					51:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study	0:55	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	9	38	gly	N-glycosylation	1455:1469	arg1	Robo1					1494:1498	Robo1	1494:1498	Robo1	1494:1498	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	1	39	from	development	210:220	arg1	system					237:242	the nervous system	225:242	the nervous system	225:242	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	7	40	theme	binding	1192:1198	arg1	affinity					1200:1207	its binding affinity	1188:1207	its binding affinity	1188:1207	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	6	41	theme	flexible	952:959	arg1	region					979:984	the highly flexible interdomain hinge region	941:984	the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1	941:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	0	42	theme	Mobility	21:28	arg1	Spectrometry					30:41	Wave Ion Mobility Spectrometry	12:41	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.	0:97	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	0	42	theme	Mobility	21:28	arg1	TWIMS					44:48	TWIMS	44:48	TWIMS	44:48	A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.
29520710	7	43	from	variations	1116:1125	arg1	size					1156:1159	size	1156:1159	size	1156:1159	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	7	43	from	variations	1116:1125	arg1	pattern					1144:1150	sulfation pattern	1134:1150	sulfation pattern	1134:1150	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	8	44	theme	CIU	1301:1303	arg1	experiments					1305:1315	CIU experiments	1301:1315	CIU experiments	1301:1315	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	1	45	theme	axon	180:183	arg1	branching					185:193	axon branching	180:193	axon branching	180:193	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	46	from	functions	288:296	arg1	tissues					317:323	the nonneuronal tissues	301:323	the nonneuronal tissues	301:323	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	3	47	theme	structural	562:571	arg1	requirements					573:584	the structural requirements	558:584	the structural requirements of HS for this interaction	558:611	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	3	47	theme	structural	562:571	arg1	unclear					623:629	unclear	623:629	unclear	623:629	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	1	48	dep	receptor	139:146	arg1	regulate					251:258	regulate	251:258	to regulate morphogenesis and many cell functions in the nonneuronal tissues	248:323	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	48	dep	receptor	139:146	arg1	regulate					156:163	regulate	156:163	to regulate axon guidance, axon branching, and dendritic development in the nervous system	153:242	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	49	theme	Roundabout	99:108	arg1	Robo1					113:117	Roundabout 1 (Robo1)	99:118	Roundabout 1 (Robo1)	99:118	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	50	theme	many	278:281	arg1	functions					288:296	many cell functions	278:296	many cell functions	278:296	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	8	51	theme	size	1388:1391	arg1	presence					1365:1372	the presence	1361:1372	the presence of a specific size and pattern of modification of HS	1361:1425	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	4	52	theme	conformational	731:744	arg1	details					746:752	the conformational details	727:752	the conformational details of the Robo1-HS interaction	727:780	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	9	53	with	conformation	1478:1489	arg1	HS					1527:1528	HS	1527:1528	HS	1527:1528	Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported.
29520710	1	54	theme	cell	283:286	arg1	functions					288:296	many cell functions	278:296	many cell functions	278:296	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	1	55	from	branching	185:193	arg1	system					237:242	the nervous system	225:242	the nervous system	225:242	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	8	56	theme	Robo1-HS	1331:1338	arg1	interaction					1340:1350	the Robo1-HS interaction	1327:1350	the Robo1-HS interaction	1327:1350	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	7	57	theme	Robo1	1269:1273	arg1	conformations					1252:1264	different conformations	1242:1264	different conformations of Robo1	1242:1273	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	1	58	from	guidance	170:177	arg1	system					237:242	the nervous system	225:242	the nervous system	225:242	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
29520710	4	59	theme	mobility	688:695	arg1	TWIMS					711:715	TWIMS	711:715	TWIMS	711:715	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	4	59	theme	mobility	688:695	arg1	spectrometry					697:708	wave ion mobility spectrometry	679:708	wave ion mobility spectrometry (TWIMS)	679:716	Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction.
29520710	3	60	theme	HS	589:590	arg1	requirements					573:584	the structural requirements	558:584	the structural requirements of HS for this interaction	558:611	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	3	60	theme	HS	589:590	arg1	unclear					623:629	unclear	623:629	unclear	623:629	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	3	60	theme	HS	589:590	arg1	details					546:552	the molecular details	532:552	the molecular details	532:552	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	6	61	theme	Robo1	1024:1028	arg1	domains					1013:1019	the Ig1 and Ig2 domains	997:1019	the Ig1 and Ig2 domains of Robo1	997:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	6	61	theme	Robo1	1024:1028	arg1	Robo1					1024:1028	Robo1	1024:1028	Robo1	1024:1028	The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction.
29520710	3	62	theme	Previous	404:411	arg1	studies					413:419	Previous studies	404:419	Previous studies	404:419	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	2	63	theme	heparan	382:388	arg1	HS					399:400	HS	399:400	HS	399:400	This interaction is known to be critically regulated by heparan sulfate (HS).
29520710	2	63	theme	heparan	382:388	arg1	sulfate					390:396	heparan sulfate	382:396	heparan sulfate (HS)	382:401	This interaction is known to be critically regulated by heparan sulfate (HS).
29520710	7	64	theme	HS	1164:1165	arg1	size					1156:1159	size	1156:1159	size	1156:1159	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	7	64	theme	HS	1164:1165	arg1	pattern					1144:1150	sulfation pattern	1134:1150	sulfation pattern	1134:1150	Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1.
29520710	8	65	theme	modification	1408:1419	arg1	presence					1365:1372	the presence	1361:1372	the presence of a specific size and pattern of modification of HS	1361:1425	Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS.
29520710	3	66	theme	molecular	536:544	arg1	unclear					623:629	unclear	623:629	unclear	623:629	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	3	66	theme	molecular	536:544	arg1	details					546:552	the molecular details	532:552	the molecular details	532:552	Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear.
29520710	1	67	theme	dendritic	200:208	arg1	development					210:220	dendritic development	200:220	dendritic development	200:220	Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues.
31242560	5	0	from	number	1089:1094	arg1	medium					1144:1149	MRS (de Man, Rogosa, and Sharpe) medium	1111:1149	MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1111:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	1	theme	added	1159:1163	arg1	oligosaccharide					1165:1179	no added oligosaccharide	1156:1179	no added oligosaccharide	1156:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	1	2	theme	enzymatic	180:188	arg1	hydrolysis					190:199	enzymatic hydrolysis	180:199	enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography	180:268	A water-soluble oligosaccharide termed EMOS-1a was prepared by enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography.
31242560	6	3	theme	natural	1291:1297	arg1	antioxidant					1299:1309	a natural antioxidant	1289:1309	a natural antioxidant in prebiotic preparations	1289:1335	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	6	3	theme	natural	1291:1297	arg1	EMOS-1a					1264:1270	the oligosaccharide EMOS-1a	1244:1270	the oligosaccharide EMOS-1a	1244:1270	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	0	4	theme	Oligosaccharides	83:98	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	4	theme	Oligosaccharides	83:98	arg1	Preparations					42:53	Prebiotic Preparations	32:53	Prebiotic Preparations	32:53	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	4	theme	Oligosaccharides	83:98	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	4	theme	Oligosaccharides	83:98	arg1	Activity					71:78	Antioxidant Activity	59:78	Antioxidant Activity	59:78	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	2	5	theme	chromatography-mass	414:432	arg1	spectrometry					434:445	gas chromatography-mass spectrometry	410:445	gas chromatography-mass spectrometry	410:445	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	6	dep	Man	1119:1121	arg1	de					1116:1117	de	1116:1117	de	1116:1117	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	6	7	theme	prebiotic	1314:1322	arg1	preparations					1324:1335	prebiotic preparations	1314:1335	prebiotic preparations	1314:1335	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	4	8	theme	ferric	938:943	arg1	power					966:970	ferric reducing antioxidant power	938:970	ferric reducing antioxidant power	938:970	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	2	9	theme	ultraviolet	339:349	arg1	spectroscopy					351:362	ultraviolet spectroscopy	339:362	ultraviolet spectroscopy	339:362	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	10	dep	Lactobacillus	1003:1015	arg1	rhamnosus					1017:1025	Lactobacillus rhamnosus	1003:1025	Lactobacillus rhamnosus	1003:1025	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	11	dep	MRS	1111:1113	arg1	Rogosa					1124:1129	Rogosa	1124:1129	Rogosa	1124:1129	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	11	dep	MRS	1111:1113	arg1	Sharpe					1136:1141	Sharpe	1136:1141	Sharpe	1136:1141	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	11	dep	MRS	1111:1113	arg1	Man					1119:1121	Man	1119:1121	Man	1119:1121	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	4	12	theme	1,1-diphenyl-2-picrylhydrazyl	835:863	arg1	activity					884:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	6	13	used	used	1281:1284	arg2	antioxidant					1299:1309	a natural antioxidant	1289:1309	a natural antioxidant in prebiotic preparations	1289:1335	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	6	13	used	used	1281:1284	arg2	EMOS-1a					1264:1270	the oligosaccharide EMOS-1a	1244:1270	the oligosaccharide EMOS-1a	1244:1270	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	4	14	theme	EMOS-1a	757:763	arg1	activities					743:752	The antioxidant activities	727:752	The antioxidant activities of EMOS-1a	727:763	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	3	15	theme	sulfate	566:572	arg1	content					574:580	the uronic acid and sulfate content	546:580	content	574:580	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	4	16	theme	antioxidant	954:964	arg1	power					966:970	ferric reducing antioxidant power	938:970	ferric reducing antioxidant power	938:970	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	3	17	theme	Chemical	516:523	arg1	analyses					525:532	Chemical analyses	516:532	Chemical analyses	516:532	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	4	18	theme	reducing	945:952	arg1	power					966:970	ferric reducing antioxidant power	938:970	ferric reducing antioxidant power	938:970	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	4	19	theme	scavenging	873:882	arg1	activity					884:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	1	20	theme	polysaccharides	204:218	arg1	hydrolysis					190:199	enzymatic hydrolysis	180:199	enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography	180:268	A water-soluble oligosaccharide termed EMOS-1a was prepared by enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography.
31242560	5	21	from	medium	1144:1149	arg1	proliferation					1201:1213	100% proliferation	1196:1213	100% proliferation	1196:1213	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	21	from	medium	1144:1149	arg1	number					1089:1094	the number	1085:1094	the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1085:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	2	22	theme	infrared	383:390	arg1	spectroscopy					392:403	Fourier-transform infrared spectroscopy	365:403	Fourier-transform infrared spectroscopy	365:403	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	3	23	theme	Da	723:724	arg1	weight					709:714	an average molecular weight	688:714	an average molecular weight of 987 Da	688:724	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	2	24	theme	Fourier-transform	365:381	arg1	spectroscopy					392:403	Fourier-transform infrared spectroscopy	365:403	Fourier-transform infrared spectroscopy	365:403	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	4	25	theme	radical	865:871	arg1	activity					884:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	5	26	theme	proliferation	986:998	arg1	level					977:981	The level	973:981	The level of proliferation of Lactobacillus rhamnosus	973:1025	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	4	27	theme	concentration-dependent	811:833	arg1	activity					884:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity	811:891	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	2	28	theme	EMOS-1a	507:513	arg1	galactose					469:477	galactose	469:477	galactose	469:477	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	2	28	theme	EMOS-1a	507:513	arg1	constituent					492:502	the main constituent	483:502	the main constituent of EMOS-1a	483:513	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	29	theme	4	1051:1051	arg1	%					1052:1052	%	1052:1052	%	1052:1052	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	1	30	theme	water-soluble	119:131	arg1	oligosaccharide					133:147	A water-soluble oligosaccharide	117:147	A water-soluble oligosaccharide termed EMOS-1a	117:162	A water-soluble oligosaccharide termed EMOS-1a was prepared by enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography.
31242560	5	31	theme	colonies	1099:1106	arg1	proliferation					1201:1213	100% proliferation	1196:1213	100% proliferation	1196:1213	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	31	theme	colonies	1099:1106	arg1	number					1089:1094	the number	1085:1094	the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1085:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	3	32	theme	uronic	550:555	arg1	acid					557:560	the uronic acid and sulfate content	546:580	acid	557:560	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	32	theme	uronic	550:555	arg1	%					601:601	5.6%	598:601	5.6%	598:601	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	1	33	theme	column	248:253	arg1	chromatography					255:268	column chromatography	248:268	column chromatography	248:268	A water-soluble oligosaccharide termed EMOS-1a was prepared by enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography.
31242560	5	34	from	colonies	1099:1106	arg1	medium					1144:1149	MRS (de Man, Rogosa, and Sharpe) medium	1111:1149	MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1111:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	2	35	theme	main	487:490	arg1	galactose					469:477	galactose	469:477	galactose	469:477	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	2	35	theme	main	487:490	arg1	constituent					492:502	the main constituent	483:502	the main constituent of EMOS-1a	483:513	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	0	36	theme	Prebiotic	32:40	arg1	Preparations					42:53	Prebiotic Preparations	32:53	Prebiotic Preparations	32:53	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	3	37	theme	average	691:697	arg1	weight					709:714	an average molecular weight	688:714	an average molecular weight of 987 Da	688:724	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	2	38	theme	chemical	275:282	arg1	structure					284:292	The chemical structure	271:292	The chemical structure of the purified fraction	271:317	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	0	39	theme	Antioxidant	59:69	arg1	Activity					71:78	Antioxidant Activity	59:78	Antioxidant Activity	59:78	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	3	40	theme	molecular	699:707	arg1	weight					709:714	an average molecular weight	688:714	an average molecular weight of 987 Da	688:724	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	41	theme	gel	634:636	arg1	chromatography					649:662	gel permeation chromatography	634:662	gel permeation chromatography	634:662	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	6	42	theme	oligosaccharide	1248:1262	arg1	antioxidant					1299:1309	a natural antioxidant	1289:1309	a natural antioxidant in prebiotic preparations	1289:1335	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	6	42	theme	oligosaccharide	1248:1262	arg1	EMOS-1a					1264:1270	the oligosaccharide EMOS-1a	1244:1270	the oligosaccharide EMOS-1a	1244:1270	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	2	43	theme	gas	410:412	arg1	spectrometry					434:445	gas chromatography-mass spectrometry	410:445	gas chromatography-mass spectrometry	410:445	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	44	theme	100	1196:1198	arg1	%					1199:1199	%	1199:1199	%	1199:1199	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	45	theme	MRS	1111:1113	arg1	medium					1144:1149	MRS (de Man, Rogosa, and Sharpe) medium	1111:1149	MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1111:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	3	46	theme	permeation	638:647	arg1	chromatography					649:662	gel permeation chromatography	634:662	gel permeation chromatography	634:662	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	47	theme	EMOS-1a	585:591	arg1	acid					557:560	the uronic acid and sulfate content	546:580	acid	557:560	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	47	theme	EMOS-1a	585:591	arg1	content					574:580	the uronic acid and sulfate content	546:580	content	574:580	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	47	theme	EMOS-1a	585:591	arg1	%					601:601	5.6%	598:601	5.6%	598:601	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	5	48	theme	±	1040:1040	arg1	%					1044:1044	1420 ± 16%	1035:1044	1420 ± 16%	1035:1044	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	49	with	medium	1144:1149	arg1	oligosaccharide					1165:1179	no added oligosaccharide	1156:1179	no added oligosaccharide	1156:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	50	theme	%	1199:1199	arg1	number					1089:1094	the number	1085:1094	the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide	1085:1179	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	50	theme	%	1199:1199	arg1	proliferation					1201:1213	100% proliferation	1196:1213	100% proliferation	1196:1213	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	4	51	theme	Trolox	894:899	arg1	capacity					924:931	Trolox equivalent antioxidant capacity	894:931	Trolox equivalent antioxidant capacity	894:931	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	2	52	theme	fraction	310:317	arg1	structure					284:292	The chemical structure	271:292	The chemical structure of the purified fraction	271:317	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	53	theme	%	1052:1052	arg1	EMOS-1a					1060:1066	4% (w/v) EMOS-1a	1051:1066	4% (w/v) EMOS-1a	1051:1066	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	5	54	theme	1420	1035:1038	arg1	%					1044:1044	1420 ± 16%	1035:1044	1420 ± 16%	1035:1044	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	2	55	theme	purified	301:308	arg1	fraction					310:317	the purified fraction	297:317	the purified fraction	297:317	The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a.
31242560	5	56	theme	Lactobacillus	1003:1015	arg1	proliferation					986:998	proliferation	986:998	proliferation of Lactobacillus rhamnosus	986:1025	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	0	57	from	Mulberries	105:114	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	57	from	Mulberries	105:114	arg1	Preparations					42:53	Prebiotic Preparations	32:53	Prebiotic Preparations	32:53	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	57	from	Mulberries	105:114	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	0	57	from	Mulberries	105:114	arg1	Activity					71:78	Antioxidant Activity	59:78	Antioxidant Activity	59:78	Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries.
31242560	4	58	theme	antioxidant	912:922	arg1	capacity					924:931	Trolox equivalent antioxidant capacity	894:931	Trolox equivalent antioxidant capacity	894:931	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	5	59	dep	%	1052:1052	arg1	w/v					1055:1057	w/v	1055:1057	w/v	1055:1057	The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation.
31242560	6	60	from	antioxidant	1299:1309	arg1	preparations					1324:1335	prebiotic preparations	1314:1335	prebiotic preparations	1314:1335	These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.
31242560	4	61	theme	equivalent	901:910	arg1	capacity					924:931	Trolox equivalent antioxidant capacity	894:931	Trolox equivalent antioxidant capacity	894:931	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	4	62	theme	antioxidant	731:741	arg1	activities					743:752	The antioxidant activities	727:752	The antioxidant activities of EMOS-1a	727:763	The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power.
31242560	3	63	contain	had	684:686	arg1	EMOS-1a					676:682	EMOS-1a	676:682	EMOS-1a	676:682	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31242560	3	63	contain	had	684:686	arg2	weight					709:714	an average molecular weight	688:714	an average molecular weight of 987 Da	688:724	Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da.
31361007	7	0	theme	brain	1788:1792	arg1	tissue					1794:1799	fixed brain tissue	1782:1799	fixed brain tissue	1782:1799	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	1	1	theme	net	430:432	arg1	scaffolds					440:448	rigid, structured perineuronal net (PNN) scaffolds	399:448	rigid, structured perineuronal net (PNN) scaffolds within the brain	399:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	2	theme	liquid	1159:1164	arg1	LC					1182:1183	LC	1182:1183	LC	1182:1183	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	2	theme	liquid	1159:1164	arg1	chromatography					1166:1179	a comprehensive liquid chromatography	1143:1179	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	3	dep	rigid	399:403	arg1	structured					406:415	structured	406:415	structured	406:415	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	4	theme	Δ6S-	1255:1258	arg1	disaccharides					1283:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	6	5	theme	CS	1550:1551	arg1	composition					1566:1576	the CS disaccharide composition	1546:1576	the CS disaccharide composition in formalin-fixed human brain tissue	1546:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	1	6	theme	PNN	435:437	arg1	scaffolds					440:448	rigid, structured perineuronal net (PNN) scaffolds	399:448	rigid, structured perineuronal net (PNN) scaffolds within the brain	399:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	7	7	theme	disaccharide	1752:1763	arg1	composition					1765:1775	the CS disaccharide composition	1745:1775	the CS disaccharide composition from fixed brain tissue	1745:1799	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	4	8	theme	disaccharides	1080:1092	arg1	percentages					1049:1059	the percentages	1045:1059	the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity	1045:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	9	theme	Δ4S6S-CS	1261:1268	arg1	disaccharides					1283:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	10	theme	long	183:186	arg1	chains					244:249	long, negatively charged, unbranched glycosaminoglycan (GAG) chains	183:249	chains	244:249	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	10	theme	long	183:186	arg1	sulfates					165:172	Chondroitin sulfates	153:172	Chondroitin sulfates (CS)	153:177	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	2	11	from	level	664:668	arg1	analysis					608:615	the analysis	604:615	the analysis of CS-GAGs in brain tissue at the disaccharide level	604:668	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	4	12	theme	chondroitin	1331:1341	arg1	activity					1349:1356	full chondroitin lyase activity	1326:1356	full chondroitin lyase activity	1326:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	5	13	theme	quantitative	1363:1374	arg1	accuracy					1376:1383	The quantitative accuracy	1359:1383	The quantitative accuracy of the five primary biologically relevant CS disaccharides	1359:1442	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	4	14	used	used	1138:1141	arg2	second					1131:1136	second	1131:1136	second	1131:1136	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	0	15	theme	mass	134:137	arg1	spectrometry					139:150	electrospray ionization-tandem mass spectrometry	103:150	electrospray ionization-tandem mass spectrometry	103:150	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	6	16	from	composition	1566:1576	arg1	tissue					1608:1613	formalin-fixed human brain tissue	1581:1613	formalin-fixed human brain tissue	1581:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	1	17	dep	chains	244:249	arg1	GAG					239:241	GAG	239:241	GAG	239:241	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	17	dep	chains	244:249	arg1	glycosaminoglycan					220:236	unbranched glycosaminoglycan	209:236	unbranched glycosaminoglycan (GAG)	209:242	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	18	theme	internal	1303:1310	arg1	validation					1312:1321	internal validation	1303:1321	internal validation by full chondroitin lyase activity	1303:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	6	19	theme	disaccharide	1553:1564	arg1	composition					1566:1576	the CS disaccharide composition	1546:1576	the CS disaccharide composition in formalin-fixed human brain tissue	1546:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	7	20	theme	diseases	1939:1946	arg1	onset					1898:1902	onset	1898:1902	onset	1898:1902	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	2	21	theme	disaccharide	651:662	arg1	level					664:668	the disaccharide level	647:668	the disaccharide level	647:668	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	1	22	theme	rigid	399:403	arg1	scaffolds					440:448	rigid, structured perineuronal net (PNN) scaffolds	399:448	rigid, structured perineuronal net (PNN) scaffolds within the brain	399:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	2	23	theme	states	542:547	arg1	range					520:524	a range	518:524	a range of pathological states, including Alzheimer's disease (AD) and schizophrenia	518:601	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	23	theme	states	542:547	arg1	disease					572:578	Alzheimer's disease	560:578	Alzheimer's disease (AD)	560:583	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	23	theme	states	542:547	arg1	schizophrenia					589:601	schizophrenia	589:601	schizophrenia	589:601	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	6	24	theme	first	1635:1639	arg1	report					1654:1659	the first quantitative report	1631:1659	the first quantitative report of CS sulfation patterns in the human brain	1631:1703	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	1	25	attach	attached	251:258	arg1	proteins					291:298	CS-proteoglycan (CSPG) core proteins	263:298	CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain	263:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	25	attach	attached	251:258	arg2	chains					244:249	long, negatively charged, unbranched glycosaminoglycan (GAG) chains	183:249	chains	244:249	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	25	attach	attached	251:258	arg2	sulfates					165:172	Chondroitin sulfates	153:172	Chondroitin sulfates (CS)	153:177	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	26	theme	Chondroitin	153:163	arg1	chains					244:249	long, negatively charged, unbranched glycosaminoglycan (GAG) chains	183:249	chains	244:249	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	26	theme	Chondroitin	153:163	arg1	CS					175:176	CS	175:176	CS	175:176	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	26	theme	Chondroitin	153:163	arg1	sulfates					165:172	Chondroitin sulfates	153:172	Chondroitin sulfates (CS)	153:177	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	27	theme	straightforward	938:952	arg1	technique					1022:1030	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	3	28	theme	tissue	836:841	arg1	samples					843:849	minute fixed tissue samples	823:849	minute fixed tissue samples with low picomolar sensitivity and high reproducibility	823:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	2	29	from	tissue	637:642	arg1	analysis					608:615	the analysis	604:615	the analysis of CS-GAGs in brain tissue at the disaccharide level	604:668	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	0	30	theme	fixed	81:85	arg1	tissue					93:98	human and rodent fixed brain tissue	64:98	human and rodent fixed brain tissue	64:98	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	7	31	theme	neurological	1926:1937	arg1	diseases					1939:1946	neurological diseases	1926:1946	neurological diseases	1926:1946	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	3	32	theme	minute	823:828	arg1	samples					843:849	minute fixed tissue samples	823:849	minute fixed tissue samples with low picomolar sensitivity and high reproducibility	823:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	4	33	theme	first	912:916	arg1	approach					918:925	The first approach	908:925	The first approach	908:925	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	34	theme	interstitial	349:360	arg1	ECMs					386:389	ECMs	386:389	ECMs	386:389	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	34	theme	interstitial	349:360	arg1	matrices					376:383	both loose interstitial extracellular matrices	338:383	both loose interstitial extracellular matrices (ECMs)	338:390	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	3	35	theme	picomolar	860:868	arg1	sensitivity					870:880	low picomolar sensitivity	856:880	low picomolar sensitivity	856:880	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	0	36	theme	electrospray	103:114	arg1	spectrometry					139:150	electrospray ionization-tandem mass spectrometry	103:150	electrospray ionization-tandem mass spectrometry	103:150	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	6	37	theme	human	1596:1600	arg1	tissue					1608:1613	formalin-fixed human brain tissue	1581:1613	formalin-fixed human brain tissue	1581:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	4	38	theme	-tandem	988:994	arg1	MS/MS					1015:1019	MS/MS	1015:1019	MS/MS	1015:1019	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	38	theme	-tandem	988:994	arg1	spectrometry					1001:1012	direct infusion (DI)-tandem mass spectrometry	968:1012	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	6	39	theme	patterns	1677:1684	arg1	report					1654:1659	the first quantitative report	1631:1659	the first quantitative report of CS sulfation patterns in the human brain	1631:1703	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	5	40	theme	CS	1427:1428	arg1	disaccharides					1430:1442	the five primary biologically relevant CS disaccharides	1388:1442	the five primary biologically relevant CS disaccharides	1388:1442	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	2	41	theme	brain	631:635	arg1	tissue					637:642	brain tissue	631:642	brain tissue	631:642	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	0	42	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of chondroitin sulfate	0:43	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	1	43	theme	CSPG	280:283	arg1	proteins					291:298	CS-proteoglycan (CSPG) core proteins	263:298	CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain	263:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	44	theme	spectrometry	1001:1012	arg1	technique					1022:1030	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	2	45	theme	CS-GAGs	620:626	arg1	analysis					608:615	the analysis	604:615	the analysis of CS-GAGs in brain tissue at the disaccharide level	604:668	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	1	46	theme	core	286:289	arg1	proteins					291:298	CS-proteoglycan (CSPG) core proteins	263:298	CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain	263:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	47	theme	infusion	975:982	arg1	MS/MS					1015:1019	MS/MS	1015:1019	MS/MS	1015:1019	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	47	theme	infusion	975:982	arg1	spectrometry					1001:1012	direct infusion (DI)-tandem mass spectrometry	968:1012	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	5	48	theme	rodent	1508:1513	arg1	tissue					1521:1526	fixed rodent brain tissue	1502:1526	fixed rodent brain tissue	1502:1526	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	0	49	theme	sulfate	37:43	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of chondroitin sulfate	0:43	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	4	50	with	disaccharides	1080:1092	arg1	validation					1312:1321	internal validation	1303:1321	internal validation by full chondroitin lyase activity	1303:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	51	theme	DI	985:986	arg1	MS/MS					1015:1019	MS/MS	1015:1019	MS/MS	1015:1019	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	51	theme	DI	985:986	arg1	spectrometry					1001:1012	direct infusion (DI)-tandem mass spectrometry	968:1012	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	5	52	theme	developmental	1466:1478	arg1	series					1492:1497	a developmental time course series	1464:1497	a developmental time course series	1464:1497	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	7	53	theme	CS-GAG	1860:1865	arg1	composition					1867:1877	the CS-GAG composition	1856:1877	the CS-GAG composition in relation to the onset and/or progression of neurological diseases	1856:1946	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	5	54	theme	course	1485:1490	arg1	series					1492:1497	a developmental time course series	1464:1497	a developmental time course series	1464:1497	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	4	55	theme	4S/6S-CS	1105:1112	arg1	ratio					1114:1118	the 4S/6S-CS ratio	1101:1118	the 4S/6S-CS ratio	1101:1118	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	56	theme	relative	1219:1226	arg1	percentages					1228:1238	the relative percentages	1215:1238	the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1215:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	57	from	component	325:333	arg1	ECMs					386:389	ECMs	386:389	ECMs	386:389	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	57	from	component	325:333	arg1	matrices					376:383	both loose interstitial extracellular matrices	338:383	both loose interstitial extracellular matrices (ECMs)	338:390	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	57	from	component	325:333	arg1	scaffolds					440:448	rigid, structured perineuronal net (PNN) scaffolds	399:448	rigid, structured perineuronal net (PNN) scaffolds within the brain	399:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	7	58	theme	fixed	1782:1786	arg1	tissue					1794:1799	fixed brain tissue	1782:1799	fixed brain tissue	1782:1799	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	2	59	contain	has	670:672	arg1	analysis					608:615	the analysis	604:615	the analysis of CS-GAGs in brain tissue at the disaccharide level	604:668	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	59	contain	has	670:672	arg2	potential					680:688	great potential	674:688	great potential to enhance disease diagnosis and prognosis	674:731	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	4	60	theme	disaccharides	1283:1295	arg1	percentages					1228:1238	the relative percentages	1215:1238	the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1215:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	61	theme	CS-proteoglycan	263:277	arg1	proteins					291:298	CS-proteoglycan (CSPG) core proteins	263:298	CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain	263:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	7	62	theme	CS	1749:1750	arg1	composition					1765:1775	the CS disaccharide composition	1745:1775	the CS disaccharide composition from fixed brain tissue	1745:1799	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	5	63	theme	primary	1397:1403	arg1	disaccharides					1430:1442	the five primary biologically relevant CS disaccharides	1388:1442	the five primary biologically relevant CS disaccharides	1388:1442	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	3	64	from	samples	843:849	arg1	disaccharides					804:816	CS disaccharides	801:816	CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility	801:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	2	65	theme	disease	701:707	arg1	diagnosis					709:717	disease diagnosis	701:717	disease diagnosis	701:717	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	4	66	dep	ratio	1114:1118	arg1	used					1138:1141	used	1138:1141	used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1138:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	67	theme	Δ4S-	1064:1067	arg1	disaccharides					1080:1092	Δ4S- and Δ6S-CS disaccharides	1064:1092	disaccharides	1080:1092	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	68	theme	full	1326:1329	arg1	activity					1349:1356	full chondroitin lyase activity	1326:1356	full chondroitin lyase activity	1326:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	6	69	theme	sulfation	1667:1675	arg1	patterns					1677:1684	CS sulfation patterns	1664:1684	CS sulfation patterns	1664:1684	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	4	70	theme	Δ6S-CS	1073:1078	arg1	disaccharides					1080:1092	Δ4S- and Δ6S-CS disaccharides	1064:1092	disaccharides	1080:1092	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	71	theme	lyase	1343:1347	arg1	activity					1349:1356	full chondroitin lyase activity	1326:1356	full chondroitin lyase activity	1326:1356	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	72	theme	unbranched	209:218	arg1	GAG					239:241	GAG	239:241	GAG	239:241	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	72	theme	unbranched	209:218	arg1	glycosaminoglycan					220:236	unbranched glycosaminoglycan	209:236	unbranched glycosaminoglycan (GAG)	209:242	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	2	73	attach	linked	508:513	arg1	range					520:524	a range	518:524	a range of pathological states, including Alzheimer's disease (AD) and schizophrenia	518:601	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	73	attach	linked	508:513	arg1	disease					572:578	Alzheimer's disease	560:578	Alzheimer's disease (AD)	560:583	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	73	attach	linked	508:513	arg2	formations					487:496	aberrant CS-PNN formations	471:496	aberrant CS-PNN formations	471:496	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	73	attach	linked	508:513	arg1	schizophrenia					589:601	schizophrenia	589:601	schizophrenia	589:601	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	3	74	with	samples	843:849	arg1	sensitivity					870:880	low picomolar sensitivity	856:880	low picomolar sensitivity	856:880	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	3	74	with	samples	843:849	arg1	reproducibility					891:905	high reproducibility	886:905	high reproducibility	886:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	6	75	theme	quantitative	1641:1652	arg1	report					1654:1659	the first quantitative report	1631:1659	the first quantitative report of CS sulfation patterns in the human brain	1631:1703	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	4	76	theme	comprehensive	1145:1157	arg1	LC					1182:1183	LC	1182:1183	LC	1182:1183	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	76	theme	comprehensive	1145:1157	arg1	chromatography					1166:1179	a comprehensive liquid chromatography	1143:1179	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	77	dep	straightforward	938:952	arg1	quantitative					955:966	quantitative	955:966	quantitative	955:966	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	2	78	theme	pathological	529:540	arg1	disease					572:578	Alzheimer's disease	560:578	Alzheimer's disease (AD)	560:583	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	78	theme	pathological	529:540	arg1	states					542:547	pathological states	529:547	pathological states	529:547	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	78	theme	pathological	529:540	arg1	schizophrenia					589:601	schizophrenia	589:601	schizophrenia	589:601	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	79	from	analysis	608:615	arg1	tissue					637:642	brain tissue	631:642	brain tissue	631:642	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	3	80	theme	-based	760:765	arg1	approaches					767:776	Two mass-spectrometry (MS)-based approaches	734:776	Two mass-spectrometry (MS)-based approaches	734:776	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	0	81	theme	rodent	74:79	arg1	tissue					93:98	human and rodent fixed brain tissue	64:98	human and rodent fixed brain tissue	64:98	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	1	82	theme	perineuronal	417:428	arg1	scaffolds					440:448	rigid, structured perineuronal net (PNN) scaffolds	399:448	rigid, structured perineuronal net (PNN) scaffolds within the brain	399:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	3	83	theme	CS	801:802	arg1	disaccharides					804:816	CS disaccharides	801:816	CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility	801:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	7	84	from	alterations	1841:1851	arg1	composition					1867:1877	the CS-GAG composition	1856:1877	the CS-GAG composition in relation to the onset and/or progression of neurological diseases	1856:1946	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	2	85	theme	aberrant	471:478	arg1	formations					487:496	aberrant CS-PNN formations	471:496	aberrant CS-PNN formations	471:496	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	86	theme	great	674:678	arg1	potential					680:688	great potential	674:688	great potential to enhance disease diagnosis and prognosis	674:731	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	2	87	from	CS-GAGs	620:626	arg1	tissue					637:642	brain tissue	631:642	brain tissue	631:642	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	0	88	theme	brain	87:91	arg1	tissue					93:98	human and rodent fixed brain tissue	64:98	human and rodent fixed brain tissue	64:98	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	4	89	theme	-MS/MS	1185:1190	arg1	technique					1192:1200	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	1	90	theme	loose	343:347	arg1	ECMs					386:389	ECMs	386:389	ECMs	386:389	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	90	theme	loose	343:347	arg1	matrices					376:383	both loose interstitial extracellular matrices	338:383	both loose interstitial extracellular matrices (ECMs)	338:390	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	3	91	theme	fixed	830:834	arg1	samples					843:849	minute fixed tissue samples	823:849	minute fixed tissue samples with low picomolar sensitivity and high reproducibility	823:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	7	92	from	composition	1867:1877	arg1	relation					1882:1889	relation	1882:1889	relation to the onset and/or progression of neurological diseases	1882:1946	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	6	93	theme	human	1693:1697	arg1	brain					1699:1703	the human brain	1689:1703	the human brain	1689:1703	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	0	94	theme	ionization-tandem	116:132	arg1	spectrometry					139:150	electrospray ionization-tandem mass spectrometry	103:150	electrospray ionization-tandem mass spectrometry	103:150	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	1	95	theme	extracellular	362:374	arg1	ECMs					386:389	ECMs	386:389	ECMs	386:389	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	95	theme	extracellular	362:374	arg1	matrices					376:383	both loose interstitial extracellular matrices	338:383	both loose interstitial extracellular matrices (ECMs)	338:390	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	6	96	theme	brain	1602:1606	arg1	tissue					1608:1613	formalin-fixed human brain tissue	1581:1613	formalin-fixed human brain tissue	1581:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	5	97	theme	relevant	1418:1425	arg1	disaccharides					1430:1442	the five primary biologically relevant CS disaccharides	1388:1442	the five primary biologically relevant CS disaccharides	1388:1442	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	4	98	theme	chromatography	1166:1179	arg1	technique					1192:1200	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	a comprehensive liquid chromatography (LC)-MS/MS technique	1143:1200	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	6	99	theme	formalin-fixed	1581:1594	arg1	tissue					1608:1613	formalin-fixed human brain tissue	1581:1613	formalin-fixed human brain tissue	1581:1613	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	3	100	theme	low	856:858	arg1	sensitivity					870:880	low picomolar sensitivity	856:880	low picomolar sensitivity	856:880	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	7	101	from	tissue	1794:1799	arg1	composition					1765:1775	the CS disaccharide composition	1745:1775	the CS disaccharide composition from fixed brain tissue	1745:1799	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	6	102	from	report	1654:1659	arg1	brain					1699:1703	the human brain	1689:1703	the human brain	1689:1703	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	5	103	theme	disaccharides	1430:1442	arg1	accuracy					1376:1383	The quantitative accuracy	1359:1383	The quantitative accuracy of the five primary biologically relevant CS disaccharides	1359:1442	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	6	104	theme	CS	1664:1665	arg1	patterns					1677:1684	CS sulfation patterns	1664:1684	CS sulfation patterns	1664:1684	We then analyzed the CS disaccharide composition in formalin-fixed human brain tissue, thus providing the first quantitative report of CS sulfation patterns in the human brain.
31361007	0	105	theme	chondroitin	25:35	arg1	sulfate					37:43	chondroitin sulfate	25:43	chondroitin sulfate	25:43	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	4	106	theme	mass	996:999	arg1	MS/MS					1015:1019	MS/MS	1015:1019	MS/MS	1015:1019	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	106	theme	mass	996:999	arg1	spectrometry					1001:1012	direct infusion (DI)-tandem mass spectrometry	968:1012	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	5	107	theme	fixed	1502:1506	arg1	tissue					1521:1526	fixed rodent brain tissue	1502:1526	fixed rodent brain tissue	1502:1526	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	4	108	theme	Δ0S-	1243:1246	arg1	disaccharides					1283:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	3	109	theme	high	886:889	arg1	reproducibility					891:905	high reproducibility	886:905	high reproducibility	886:905	Two mass-spectrometry (MS)-based approaches were adapted to detect CS disaccharides from minute fixed tissue samples with low picomolar sensitivity and high reproducibility.
31361007	4	110	theme	direct	968:973	arg1	MS/MS					1015:1019	MS/MS	1015:1019	MS/MS	1015:1019	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	4	110	theme	direct	968:973	arg1	spectrometry					1001:1012	direct infusion (DI)-tandem mass spectrometry	968:1012	a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique	936:1030	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	5	111	theme	brain	1515:1519	arg1	tissue					1521:1526	fixed rodent brain tissue	1502:1526	fixed rodent brain tissue	1502:1526	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	4	112	theme	Δ4S-	1249:1252	arg1	disaccharides					1283:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
31361007	0	113	theme	human	64:68	arg1	tissue					93:98	human and rodent fixed brain tissue	64:98	human and rodent fixed brain tissue	64:98	Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.
31361007	2	114	theme	CS-PNN	480:485	arg1	formations					487:496	aberrant CS-PNN formations	471:496	aberrant CS-PNN formations	471:496	As aberrant CS-PNN formations have been linked to a range of pathological states, including Alzheimer's disease (AD) and schizophrenia, the analysis of CS-GAGs in brain tissue at the disaccharide level has great potential to enhance disease diagnosis and prognosis.
31361007	7	115	dep	onset	1898:1902	arg1	the					1894:1896	the	1894:1896	the	1894:1896	The ability to comprehensively analyze the CS disaccharide composition from fixed brain tissue provides a means with which to identify alterations in the CS-GAG composition in relation to the onset and/or progression of neurological diseases.
31361007	5	116	theme	time	1480:1483	arg1	series					1492:1497	a developmental time course series	1464:1497	a developmental time course series	1464:1497	The quantitative accuracy of the five primary biologically relevant CS disaccharides was validated using a developmental time course series in fixed rodent brain tissue.
31361007	1	117	dep	long	183:186	arg1	charged					200:206	charged	200:206	charged	200:206	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	1	118	theme	glycan	318:323	arg1	component					325:333	the glycan component	314:333	the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain	314:465	Chondroitin sulfates (CS) are long, negatively charged, unbranched glycosaminoglycan (GAG) chains attached to CS-proteoglycan (CSPG) core proteins that comprise the glycan component in both loose interstitial extracellular matrices (ECMs) and in rigid, structured perineuronal net (PNN) scaffolds within the brain.
31361007	4	119	theme	Δ2S6S-CS	1274:1281	arg1	disaccharides					1283:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides	1243:1295	The first approach employed a straightforward, quantitative direct infusion (DI)-tandem mass spectrometry (MS/MS) technique to determine the percentages of Δ4S- and Δ6S-CS disaccharides within the 4S/6S-CS ratio, while the second used a comprehensive liquid chromatography (LC)-MS/MS technique to determine the relative percentages of Δ0S-, Δ4S-, Δ6S-, Δ4S6S-CS and Δ2S6S-CS disaccharides, with internal validation by full chondroitin lyase activity.
29628238	0	0	theme	drug	64:67	arg1	characteristics					77:91	drug release characteristics	64:91	drug release characteristics	64:91	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	4	1	theme	compact	714:720	arg1	structure					730:738	a compact network structure	712:738	a compact network structure	712:738	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	3	2	theme	Infrared	450:457	arg1	Spectroscopy					459:470	Infrared Spectroscopy	450:470	Infrared Spectroscopy	450:470	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	0	3	from	Effects	0:6	arg1	characteristics					77:91	drug release characteristics	64:91	drug release characteristics	64:91	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	0	3	from	Effects	0:6	arg1	properties					48:57	properties	48:57	properties	48:57	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	0	3	from	Effects	0:6	arg1	structure					37:45	structure	37:45	structure	37:45	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	5	4	theme	drug	853:856	arg1	ciprofloxacin					858:870	model drug ciprofloxacin	847:870	model drug ciprofloxacin	847:870	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	6	5	theme	release	957:963	arg1	capacity					965:972	sustained release capacity	947:972	sustained release capacity	947:972	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	0	6	theme	release	69:75	arg1	characteristics					77:91	drug release characteristics	64:91	drug release characteristics	64:91	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	2	7	theme	konjac	247:252	arg1	KGM					267:269	KGM	267:269	KGM	267:269	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	2	7	theme	konjac	247:252	arg1	glucomannan					254:264	konjac glucomannan	247:264	konjac glucomannan (KGM)	247:270	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	6	8	theme	sustained	947:955	arg1	capacity					965:972	sustained release capacity	947:972	sustained release capacity	947:972	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	0	9	theme	agarose	96:102	arg1	hydrogels					104:112	agarose hydrogels	96:112	agarose hydrogels	96:112	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	5	10	dep	In	741:742	arg1	vitro					744:748	vitro	744:748	vitro	744:748	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	5	11	theme	hydrogels	785:793	arg1	behavior					763:770	In vitro drug release behavior	741:770	In vitro drug release behavior of composite hydrogels	741:793	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	3	12	theme	X-ray	473:477	arg1	Diffraction					479:489	X-ray Diffraction	473:489	X-ray Diffraction	473:489	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	3	13	from	effect	328:333	arg1	properties					363:372	properties	363:372	properties	363:372	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	3	13	from	effect	328:333	arg1	structure					349:357	structure	349:357	structure	349:357	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	7	14	dep	properties	1099:1108	arg1	the					1095:1097	the	1095:1097	the	1095:1097	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	2	15	used	used	276:279	arg2	KGM					267:269	KGM	267:269	KGM	267:269	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	2	15	used	used	276:279	arg2	glucomannan					254:264	konjac glucomannan	247:264	konjac glucomannan (KGM)	247:270	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	3	16	dep	Fourier	432:438	arg1	Transform					440:448	Transform	440:448	Transform Infrared Spectroscopy	440:470	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	1	17	theme	strong	148:153	arg1	rigidity					155:162	rigidity	155:162	rigidity	155:162	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	5	18	theme	drug	750:753	arg1	behavior					763:770	In vitro drug release behavior	741:770	In vitro drug release behavior of composite hydrogels	741:793	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	4	19	theme	KGM	608:610	arg1	concentration					612:624	KGM concentration	608:624	KGM concentration	608:624	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	5	20	theme	release	755:761	arg1	behavior					763:770	In vitro drug release behavior	741:770	In vitro drug release behavior of composite hydrogels	741:793	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	3	21	dep	structure	349:357	arg1	the					345:347	the	345:347	the	345:347	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	5	22	theme	different	818:826	arg1	environments					828:839	different environments	818:839	different environments using model drug ciprofloxacin	818:870	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	6	23	theme	KGM	1028:1030	arg1	incorporation					1011:1023	the incorporation	1007:1023	the incorporation of KGM	1007:1030	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	0	24	theme	glucomannan	18:28	arg1	Effects					0:6	Effects	0:6	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels	0:112	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	3	25	theme	Scanning	496:503	arg1	Microscopy					514:523	Scanning Electron Microscopy	496:523	Scanning Electron Microscopy	496:523	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	4	26	theme	hydrogels	583:591	arg1	flexibility					554:564	the flexibility	550:564	the flexibility of the composite hydrogels	550:591	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	0	27	theme	konjac	11:16	arg1	glucomannan					18:28	konjac glucomannan	11:28	konjac glucomannan	11:28	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	0	28	theme	hydrogels	104:112	arg1	characteristics					77:91	drug release characteristics	64:91	drug release characteristics	64:91	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	0	28	theme	hydrogels	104:112	arg1	properties					48:57	properties	48:57	properties	48:57	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	0	28	theme	hydrogels	104:112	arg1	structure					37:45	structure	37:45	structure	37:45	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	6	29	dep	encapsulation	901:913	arg1	the					897:899	the	897:899	the	897:899	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	3	30	theme	Electron	505:512	arg1	Microscopy					514:523	Scanning Electron Microscopy	496:523	Scanning Electron Microscopy	496:523	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	4	31	theme	composite	573:581	arg1	hydrogels					583:591	the composite hydrogels	569:591	the composite hydrogels	569:591	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	6	32	theme	loading	921:927	arg1	efficiencies					929:940	drug loading efficiencies	916:940	drug loading efficiencies	916:940	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	7	33	theme	AG	1146:1147	arg1	hydrogels					1149:1157	AG hydrogels	1146:1157	AG hydrogels	1146:1157	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	3	34	theme	hydrogels	380:388	arg1	properties					363:372	properties	363:372	properties	363:372	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	3	34	theme	hydrogels	380:388	arg1	structure					349:357	structure	349:357	structure	349:357	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	7	35	theme	drug	1114:1117	arg1	characteristics					1127:1141	drug release characteristics	1114:1141	drug release characteristics	1114:1141	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	6	36	theme	drug	916:919	arg1	efficiencies					929:940	drug loading efficiencies	916:940	drug loading efficiencies	916:940	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	0	37	dep	structure	37:45	arg1	the					33:35	the	33:35	the	33:35	Effects of konjac glucomannan on the structure, properties, and drug release characteristics of agarose hydrogels.
29628238	3	38	theme	KGM	338:340	arg1	effect					328:333	The effect	324:333	The effect of KGM on the structure and properties of AG hydrogels	324:388	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	5	39	theme	model	847:851	arg1	ciprofloxacin					858:870	model drug ciprofloxacin	847:870	model drug ciprofloxacin	847:870	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	1	40	contain	have	143:146	arg2	rigidity					155:162	rigidity	155:162	rigidity	155:162	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	1	40	contain	have	143:146	arg2	brittleness					168:178	brittleness	168:178	brittleness	168:178	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	1	40	contain	have	143:146	arg1	hydrogels					133:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	4	41	theme	synergistic	656:666	arg1	interaction					668:678	a synergistic interaction	654:678	a synergistic interaction between KGM and AG resulting in a compact network structure	654:738	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	1	42	theme	AG	129:130	arg1	hydrogels					133:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	7	43	theme	release	1119:1125	arg1	characteristics					1127:1141	drug release characteristics	1114:1141	drug release characteristics	1114:1141	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	1	44	theme	Pure	115:118	arg1	hydrogels					133:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	4	45	theme	network	722:728	arg1	structure					730:738	a compact network structure	712:738	a compact network structure	712:738	The results showed that the flexibility of the composite hydrogels increases with KGM concentration, which may be attributed to a synergistic interaction between KGM and AG resulting in a compact network structure.
29628238	1	46	theme	agarose	120:126	arg1	hydrogels					133:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels	115:141	Pure agarose (AG) hydrogels have strong rigidity and brittleness, which greatly limit their applications.
29628238	7	47	contain	has	1066:1068	arg1	KGM					1062:1064	KGM	1062:1064	KGM	1062:1064	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	7	47	contain	has	1066:1068	arg2	potential					1074:1082	the potential to enhance the properties and drug release characteristics of AG hydrogels	1070:1157	the potential to enhance the properties and drug release characteristics of AG hydrogels	1070:1157	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	3	48	theme	rotational	410:419	arg1	rheometry					421:429	rotational rheometry	410:429	rotational rheometry	410:429	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	5	49	theme	composite	775:783	arg1	hydrogels					785:793	composite hydrogels	775:793	composite hydrogels	775:793	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	2	50	theme	hydrogels	313:321	arg1	properties					296:305	the properties	292:305	the properties of AG hydrogels	292:321	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	3	51	theme	AG	377:378	arg1	hydrogels					380:388	AG hydrogels	377:388	AG hydrogels	377:388	The effect of KGM on the structure and properties of AG hydrogels was investigated by rotational rheometry, Fourier Transform Infrared Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy.
29628238	6	52	theme	hydrogels	980:988	arg1	capacity					965:972	sustained release capacity	947:972	sustained release capacity	947:972	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	6	52	theme	hydrogels	980:988	arg1	efficiencies					929:940	drug loading efficiencies	916:940	drug loading efficiencies	916:940	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	6	52	theme	hydrogels	980:988	arg1	encapsulation					901:913	encapsulation	901:913	encapsulation	901:913	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29628238	5	53	theme	In	741:742	arg1	behavior					763:770	In vitro drug release behavior	741:770	In vitro drug release behavior of composite hydrogels	741:793	In vitro drug release behavior of composite hydrogels was investigated under different environments using model drug ciprofloxacin.
29628238	7	54	theme	hydrogels	1149:1157	arg1	characteristics					1127:1141	drug release characteristics	1114:1141	drug release characteristics	1114:1141	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	7	54	theme	hydrogels	1149:1157	arg1	properties					1099:1108	properties	1099:1108	properties	1099:1108	These results suggested that KGM has the potential to enhance the properties and drug release characteristics of AG hydrogels.
29628238	2	55	theme	AG	310:311	arg1	hydrogels					313:321	AG hydrogels	310:321	AG hydrogels	310:321	Therefore, in this study, konjac glucomannan (KGM) was used to improve the properties of AG hydrogels.
29628238	6	56	theme	AG	977:978	arg1	hydrogels					980:988	AG hydrogels	977:988	AG hydrogels	977:988	The results showed that the encapsulation, drug loading efficiencies, and sustained release capacity of AG hydrogels were enhanced by the incorporation of KGM.
29551513	5	0	from	90°C	784:787	arg1	G					778:778	G'	778:779	G'	778:779	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	2	1	theme	starches	311:318	arg1	shear					258:262	steady shear	251:262	steady shear	251:262	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	2	1	theme	starches	311:318	arg1	properties					288:297	dynamic oscillatory properties	268:297	dynamic oscillatory properties	268:297	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	0	2	from	properties	12:21	arg1	relation					26:33	relation	26:33	relation to molecular structure of quinoa starch	26:73	Rheological properties in relation to molecular structure of quinoa starch.
29551513	2	3	theme	quinoa	304:309	arg1	starches					311:318	9 quinoa starches	302:318	9 quinoa starches varying in composition and structure	302:355	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	4	4	theme	4-stage	535:541	arg1	process					543:549	a 4-stage process	533:549	a 4-stage process including 2 phase transitions	533:579	Temperature sweep analysis reveals that the quinoa starch encounters a 4-stage process including 2 phase transitions.
29551513	4	5	theme	Temperature	464:474	arg1	analysis					482:489	Temperature sweep analysis	464:489	Temperature sweep analysis	464:489	Temperature sweep analysis reveals that the quinoa starch encounters a 4-stage process including 2 phase transitions.
29551513	2	6	theme	dynamic	268:274	arg1	properties					288:297	dynamic oscillatory properties	268:297	dynamic oscillatory properties	268:297	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	6	7	theme	super-long	853:862	arg1	chains					869:874	super-long unit chains	853:874	super-long unit chains of amylopectin	853:889	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	0	8	theme	Rheological	0:10	arg1	properties					12:21	Rheological properties	0:21	Rheological properties in relation to molecular structure of quinoa starch	0:73	Rheological properties in relation to molecular structure of quinoa starch.
29551513	3	9	theme	selected	436:443	arg1	models					456:461	4 selected mathematic models	434:461	4 selected mathematic models	434:461	Steady shear analysis shows that the flow curves could be well described by 4 selected mathematic models.
29551513	5	10	theme	relationship	601:612	arg1	analysis					614:621	Structure-function relationship analysis	582:621	Structure-function relationship analysis	582:621	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	11	theme	Structure-function	582:599	arg1	analysis					614:621	Structure-function relationship analysis	582:621	Structure-function relationship analysis	582:621	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	3	12	theme	flow	395:398	arg1	curves					400:405	the flow curves	391:405	the flow curves	391:405	Steady shear analysis shows that the flow curves could be well described by 4 selected mathematic models.
29551513	6	13	theme	chains	842:847	arg1	roles					812:816	The roles	808:816	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch	808:948	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	6	14	theme	quinoa	936:941	arg1	starch					943:948	quinoa starch	936:948	quinoa starch	936:948	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	1	15	theme	Quinoa	76:81	arg1	granules					90:97	Quinoa starch granules	76:97	Quinoa starch granules	76:97	Quinoa starch granules are small (~0.5 - 3μm) with potentials for some food and other applications.
29551513	1	16	theme	food	147:150	arg1	applications					162:173	some food and other applications	142:173	applications	162:173	Quinoa starch granules are small (~0.5 - 3μm) with potentials for some food and other applications.
29551513	5	17	contain	have	717:720	arg1	unit					658:661	unit	658:661	unit	658:661	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	17	contain	have	717:720	arg1	composition					635:645	composition	635:645	composition as well as unit and internal chain length distribution of amylopectin	635:715	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	17	contain	have	717:720	arg2	impact					734:739	significant impact	722:739	significant impact	722:739	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	7	18	theme	rheology	1055:1062	arg1	basis					1039:1043	the structural basis	1024:1043	the structural basis of starch rheology	1024:1062	This study may stimulate further interest in understanding the structural basis of starch rheology.
29551513	1	19	theme	starch	83:88	arg1	granules					90:97	Quinoa starch granules	76:97	Quinoa starch granules	76:97	Quinoa starch granules are small (~0.5 - 3μm) with potentials for some food and other applications.
29551513	1	20	with	small	103:107	arg1	potentials					127:136	potentials	127:136	potentials	127:136	Quinoa starch granules are small (~0.5 - 3μm) with potentials for some food and other applications.
29551513	6	21	theme	starch	943:948	arg1	properties					922:931	the rheological properties	906:931	the rheological properties of quinoa starch	906:948	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	5	22	dep	G	778:778	arg1	e.g.					772:775	e.g.	772:775	e.g.	772:775	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	1	23	theme	other	156:160	arg1	applications					162:173	some food and other applications	142:173	applications	162:173	Quinoa starch granules are small (~0.5 - 3μm) with potentials for some food and other applications.
29551513	4	24	theme	quinoa	508:513	arg1	starch					515:520	the quinoa starch	504:520	the quinoa starch	504:520	Temperature sweep analysis reveals that the quinoa starch encounters a 4-stage process including 2 phase transitions.
29551513	5	25	theme	length	682:687	arg1	distribution					689:700	internal chain length distribution	667:700	internal chain length distribution	667:700	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	26	theme	quinoa	793:798	arg1	starch					800:805	quinoa starch	793:805	quinoa starch	793:805	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	7	27	theme	starch	1048:1053	arg1	rheology					1055:1062	starch rheology	1048:1062	starch rheology	1048:1062	This study may stimulate further interest in understanding the structural basis of starch rheology.
29551513	6	28	theme	individual	826:835	arg1	chains					842:847	some individual unit chains	821:847	some individual unit chains	821:847	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	2	29	theme	steady	251:256	arg1	shear					258:262	steady shear	251:262	steady shear	251:262	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	5	30	theme	starch	800:805	arg1	properties					760:769	the rheological properties	744:769	the rheological properties (e.g., G' at 90°C) of quinoa starch	744:805	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	0	31	theme	molecular	38:46	arg1	structure					48:56	molecular structure	38:56	molecular structure of quinoa starch	38:73	Rheological properties in relation to molecular structure of quinoa starch.
29551513	7	32	theme	structural	1028:1037	arg1	basis					1039:1043	the structural basis	1024:1043	the structural basis of starch rheology	1024:1062	This study may stimulate further interest in understanding the structural basis of starch rheology.
29551513	6	33	theme	rheological	910:920	arg1	properties					922:931	the rheological properties	906:931	the rheological properties of quinoa starch	906:948	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	5	34	theme	amylopectin	705:715	arg1	distribution					689:700	internal chain length distribution	667:700	internal chain length distribution	667:700	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	34	theme	amylopectin	705:715	arg1	unit					658:661	unit	658:661	unit	658:661	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	5	34	theme	amylopectin	705:715	arg1	composition					635:645	composition	635:645	composition as well as unit and internal chain length distribution of amylopectin	635:715	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	4	35	theme	sweep	476:480	arg1	analysis					482:489	Temperature sweep analysis	464:489	Temperature sweep analysis	464:489	Temperature sweep analysis reveals that the quinoa starch encounters a 4-stage process including 2 phase transitions.
29551513	3	36	theme	Steady	358:363	arg1	analysis					371:378	Steady shear analysis	358:378	Steady shear analysis	358:378	Steady shear analysis shows that the flow curves could be well described by 4 selected mathematic models.
29551513	5	37	theme	rheological	748:758	arg1	properties					760:769	the rheological properties	744:769	the rheological properties (e.g., G' at 90°C) of quinoa starch	744:805	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	2	38	dep	shear	258:262	arg1	the					247:249	the	247:249	the	247:249	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	3	39	theme	shear	365:369	arg1	analysis					371:378	Steady shear analysis	358:378	Steady shear analysis	358:378	Steady shear analysis shows that the flow curves could be well described by 4 selected mathematic models.
29551513	6	40	theme	chains	869:874	arg1	roles					812:816	The roles	808:816	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch	808:948	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	3	41	theme	mathematic	445:454	arg1	models					456:461	4 selected mathematic models	434:461	4 selected mathematic models	434:461	Steady shear analysis shows that the flow curves could be well described by 4 selected mathematic models.
29551513	5	42	theme	internal	667:674	arg1	distribution					689:700	internal chain length distribution	667:700	internal chain length distribution	667:700	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	6	43	theme	unit	837:840	arg1	chains					842:847	some individual unit chains	821:847	some individual unit chains	821:847	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	5	44	theme	chain	676:680	arg1	distribution					689:700	internal chain length distribution	667:700	internal chain length distribution	667:700	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
29551513	2	45	theme	oscillatory	276:286	arg1	properties					288:297	dynamic oscillatory properties	268:297	dynamic oscillatory properties	268:297	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	4	46	theme	phase	563:567	arg1	transitions					569:579	2 phase transitions	561:579	2 phase transitions	561:579	Temperature sweep analysis reveals that the quinoa starch encounters a 4-stage process including 2 phase transitions.
29551513	0	47	theme	starch	68:73	arg1	structure					48:56	molecular structure	38:56	molecular structure of quinoa starch	38:73	Rheological properties in relation to molecular structure of quinoa starch.
29551513	2	48	theme	starch	206:211	arg1	resource					213:220	a new starch resource	200:220	a new starch resource	200:220	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	2	48	theme	starch	206:211	arg1	it					194:195	it	194:195	it	194:195	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	6	49	theme	unit	864:867	arg1	chains					869:874	super-long unit chains	853:874	super-long unit chains of amylopectin	853:889	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	0	50	theme	quinoa	61:66	arg1	starch					68:73	quinoa starch	61:73	quinoa starch	61:73	Rheological properties in relation to molecular structure of quinoa starch.
29551513	6	51	theme	amylopectin	879:889	arg1	chains					869:874	super-long unit chains	853:874	super-long unit chains of amylopectin	853:889	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	6	51	theme	amylopectin	879:889	arg1	chains					842:847	some individual unit chains	821:847	some individual unit chains	821:847	The roles of some individual unit chains and super-long unit chains of amylopectin in determining the rheological properties of quinoa starch were revealed.
29551513	2	52	theme	new	202:204	arg1	resource					213:220	a new starch resource	200:220	a new starch resource	200:220	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	2	52	theme	new	202:204	arg1	it					194:195	it	194:195	it	194:195	To better exploit it as a new starch resource, this study investigates the steady shear and dynamic oscillatory properties of 9 quinoa starches varying in composition and structure.
29551513	5	53	theme	significant	722:732	arg1	impact					734:739	significant impact	722:739	significant impact	722:739	Structure-function relationship analysis showed that composition as well as unit and internal chain length distribution of amylopectin have significant impact on the rheological properties (e.g., G' at 90°C) of quinoa starch.
30599998	7	0	theme	defect-cecum	1129:1140	arg1	abrasion					1142:1149	abdominal defect-cecum abrasion	1119:1149	abdominal defect-cecum abrasion	1119:1149	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	4	1	theme	excellent	678:686	arg1	functionality					702:714	excellent antimicrobial functionality	678:714	excellent antimicrobial functionality against S. aureus and E. coli bacteria	678:753	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	3	2	theme	N	496:496	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	1	3	theme	notable	257:263	arg1	influences					265:274	notable influences	257:274	notable influences	257:274	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	7	4	theme	composite	1201:1209	arg1	gauze					1211:1215	O-CS/ORC composite gauze	1192:1215	O-CS/ORC composite gauze	1192:1215	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	7	4	theme	composite	1201:1209	arg1	N					1189:1189	N	1189:1189	N	1189:1189	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	7	5	theme	abdominal	1119:1127	arg1	abrasion					1142:1149	abdominal defect-cecum abrasion	1119:1149	abdominal defect-cecum abrasion	1119:1149	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	9	6	dep	gauze	1459:1463	arg1	O-CS/ORC					1440:1447	the N, O-CS/ORC composite gauze	1433:1463	O-CS/ORC	1440:1447	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	9	7	theme	ORC	1562:1564	arg1	activity					1538:1545	activity	1538:1545	activity of N, O-CS and ORC	1538:1564	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	8	8	theme	O-CS/ORC	1297:1304	arg1	gauze					1316:1320	the N, O-CS/ORC composite gauze	1290:1320	gauze	1316:1320	The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions.
30599998	9	9	theme	potential	1396:1404	arg1	mechanism					1420:1428	the potential anti-adhesion mechanism	1392:1428	the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC,	1392:1565	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	4	10	theme	composite	615:623	arg1	degradable					634:643	degradable	634:643	degradable	634:643	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	4	10	theme	composite	615:623	arg1	gauze					625:629	The N, O-CS/ORC composite gauze	599:629	gauze	625:629	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	9	11	theme	function	1516:1523	arg1	combination					1493:1503	the combination	1489:1503	the combination of barrier function	1489:1523	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	3	12	theme	N	556:556	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	6	13	theme	normal	955:960	arg1	cells					962:966	normal cells	955:966	normal cells	955:966	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	5	14	theme	composite	817:825	arg1	gauze					827:831	the N, O-CS/ORC composite gauze	801:831	gauze	827:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	2	15	theme	effective	414:422	arg1	barrier					324:330	A physical barrier	313:330	A physical barrier placed between the wound site and the adjacent tissues	313:385	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	2	15	theme	effective	414:422	arg1	technique					424:432	a convenient and highly effective technique	390:432	a convenient and highly effective technique to minimize or prevent abdominal adhesions	390:475	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	3	16	theme	regenerated	533:543	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	1	17	theme	common	176:181	arg1	complications					183:195	the most common complications	167:195	the most common complications after surgery	167:209	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	0	18	theme	postoperative	117:129	arg1	adhesion					131:138	postoperative adhesion	117:138	postoperative adhesion	117:138	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	7	19	used	used	1156:1159	arg2	models					1108:1113	the rat models	1100:1113	the rat models for abdominal defect-cecum abrasion	1100:1149	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	3	20	theme	cellulose	545:553	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	1	21	theme	complications	183:195	arg1	complications					183:195	the most common complications	167:195	the most common complications after surgery	167:209	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	1	21	theme	complications	183:195	arg1	one					160:162	one	160:162	one	160:162	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	7	22	theme	tissue	1231:1236	arg1	adhesions					1238:1246	tissue adhesions	1231:1246	tissue adhesions	1231:1246	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	5	23	theme	rabbit	850:855	arg1	livers/ears					857:867	rabbit livers/ears	850:867	rabbit livers/ears as models	850:877	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	0	24	theme	Biodegradable	0:12	arg1	N					14:14	Biodegradable N	0:14	Biodegradable N	0:14	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	0	24	theme	Biodegradable	0:12	arg1	composite					73:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	1	25	theme	damaged	286:292	arg1	tissue					294:299	the damaged tissue	282:299	the damaged tissue	282:299	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	9	26	theme	N	1550:1550	arg1	activity					1538:1545	activity	1538:1545	activity of N, O-CS and ORC	1538:1564	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	2	27	theme	physical	315:322	arg1	technique					424:432	a convenient and highly effective technique	390:432	a convenient and highly effective technique to minimize or prevent abdominal adhesions	390:475	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	2	27	theme	physical	315:322	arg1	barrier					324:330	A physical barrier	313:330	A physical barrier placed between the wound site and the adjacent tissues	313:385	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	9	28	theme	N	1437:1437	arg1	gauze					1459:1463	the N, O-CS/ORC composite gauze	1433:1463	gauze	1459:1463	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	0	29	theme	O-carboxymethyl	17:31	arg1	N					14:14	Biodegradable N	0:14	Biodegradable N	0:14	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	0	29	theme	O-carboxymethyl	17:31	arg1	composite					73:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	3	30	dep	gauze	579:583	arg1	O-CS/ORC					559:566	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	O-CS/ORC	559:566	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	6	31	theme	cells	1012:1016	arg1	adhesion					989:996	the adhesion	985:996	the adhesion of fibroblast cells	985:1016	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	5	32	theme	O-CS/ORC	808:815	arg1	gauze					827:831	the N, O-CS/ORC composite gauze	801:831	gauze	827:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	3	33	theme	composite	569:577	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	9	34	theme	anti-adhesion	1406:1418	arg1	mechanism					1420:1428	the potential anti-adhesion mechanism	1392:1428	the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC,	1392:1565	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	2	35	theme	adjacent	370:377	arg1	tissues					379:385	the adjacent tissues	366:385	the adjacent tissues	366:385	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	0	36	theme	regenerated	51:61	arg1	N					14:14	Biodegradable N	0:14	Biodegradable N	0:14	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	0	36	theme	regenerated	51:61	arg1	composite					73:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	4	37	theme	antimicrobial	688:700	arg1	functionality					702:714	excellent antimicrobial functionality	678:714	excellent antimicrobial functionality against S. aureus and E. coli bacteria	678:753	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	1	38	dep	causing	249:255	arg1	healed					305:310	healed	305:310	causing notable influences after the damaged tissue has healed	249:310	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	9	39	theme	O-CS	1553:1556	arg1	activity					1538:1545	activity	1538:1545	activity of N, O-CS and ORC	1538:1564	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	5	40	theme	gauze	827:831	arg1	efficacy					789:796	the notable hemostatic efficacy	766:796	the notable hemostatic efficacy of the N, O-CS/ORC composite gauze	766:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	2	41	theme	convenient	392:401	arg1	barrier					324:330	A physical barrier	313:330	A physical barrier placed between the wound site and the adjacent tissues	313:385	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	2	41	theme	convenient	392:401	arg1	technique					424:432	a convenient and highly effective technique	390:432	a convenient and highly effective technique to minimize or prevent abdominal adhesions	390:475	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	6	42	theme	composite	920:928	arg1	nontoxic					939:946	nontoxic	939:946	nontoxic	939:946	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	6	42	theme	composite	920:928	arg1	gauze					930:934	the N, O-CS/ORC composite gauze	904:934	gauze	930:934	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	0	43	theme	chitosan/oxidized	33:49	arg1	N					14:14	Biodegradable N	0:14	Biodegradable N	0:14	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	0	43	theme	chitosan/oxidized	33:49	arg1	composite					73:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	9	44	theme	gauze	1459:1463	arg1	mechanism					1420:1428	the potential anti-adhesion mechanism	1392:1428	the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC,	1392:1565	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	5	45	theme	N	805:805	arg1	gauze					827:831	the N, O-CS/ORC composite gauze	801:831	gauze	827:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	1	46	theme	abdominal	229:237	arg1	surgery					239:245	abdominal surgery	229:245	abdominal surgery	229:245	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	5	47	theme	hemostatic	778:787	arg1	efficacy					789:796	the notable hemostatic efficacy	766:796	the notable hemostatic efficacy of the N, O-CS/ORC composite gauze	766:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	6	48	theme	O-CS/ORC	911:918	arg1	nontoxic					939:946	nontoxic	939:946	nontoxic	939:946	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	6	48	theme	O-CS/ORC	911:918	arg1	gauze					930:934	the N, O-CS/ORC composite gauze	904:934	gauze	930:934	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	6	49	theme	fibroblast	1001:1010	arg1	cells					1012:1016	fibroblast cells	1001:1016	fibroblast cells	1001:1016	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	9	50	theme	composite	1449:1457	arg1	gauze					1459:1463	the N, O-CS/ORC composite gauze	1433:1463	gauze	1459:1463	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	2	51	theme	wound	351:355	arg1	site					357:360	the wound site	347:360	the wound site	347:360	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	0	52	theme	cellulose	63:71	arg1	N					14:14	Biodegradable N	0:14	Biodegradable N	0:14	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	0	52	theme	cellulose	63:71	arg1	composite					73:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	O-carboxymethyl chitosan/oxidized regenerated cellulose composite	17:81	Biodegradable N, O-carboxymethyl chitosan/oxidized regenerated cellulose composite gauze as a barrier for preventing postoperative adhesion.
30599998	2	53	theme	abdominal	457:465	arg1	adhesions					467:475	abdominal adhesions	457:475	abdominal adhesions	457:475	A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions.
30599998	1	54	theme	Tissue	141:146	arg1	adhesion					148:155	Tissue adhesion	141:155	Tissue adhesion	141:155	Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed.
30599998	6	55	theme	N	908:908	arg1	nontoxic					939:946	nontoxic	939:946	nontoxic	939:946	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	6	55	theme	N	908:908	arg1	gauze					930:934	the N, O-CS/ORC composite gauze	904:934	gauze	930:934	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	7	56	theme	rat	1104:1106	arg1	models					1108:1113	the rat models	1100:1113	the rat models for abdominal defect-cecum abrasion	1100:1149	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	8	57	theme	peritoneal	1361:1370	arg1	adhesions					1372:1380	postsurgical peritoneal adhesions	1348:1380	postsurgical peritoneal adhesions	1348:1380	The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions.
30599998	8	58	theme	postsurgical	1348:1359	arg1	adhesions					1372:1380	postsurgical peritoneal adhesions	1348:1380	postsurgical peritoneal adhesions	1348:1380	The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions.
30599998	4	59	theme	N	603:603	arg1	degradable					634:643	degradable	634:643	degradable	634:643	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	4	59	theme	N	603:603	arg1	gauze					625:629	The N, O-CS/ORC composite gauze	599:629	gauze	625:629	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	8	60	theme	composite	1306:1314	arg1	gauze					1316:1320	the N, O-CS/ORC composite gauze	1290:1320	gauze	1316:1320	The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions.
30599998	9	61	theme	barrier	1508:1514	arg1	function					1516:1523	barrier function	1508:1523	barrier function	1508:1523	Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
30599998	4	62	dep	aureus	727:732	arg1	bacteria					746:753	bacteria	746:753	bacteria	746:753	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	8	63	theme	N	1294:1294	arg1	gauze					1316:1320	the N, O-CS/ORC composite gauze	1290:1320	gauze	1316:1320	The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions.
30599998	7	64	theme	N	1189:1189	arg1	efficacy					1177:1184	the efficacy	1173:1184	the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery	1173:1260	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	6	65	theme	tissue	1070:1075	arg1	adhesion					1077:1084	tissue adhesion	1070:1084	tissue adhesion	1070:1084	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	3	66	theme	O-carboxymethyl	499:513	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	6	67	theme	potential	1042:1050	arg1	use					1052:1054	its potential use	1038:1054	its potential use	1038:1054	The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion.
30599998	4	68	theme	O-CS/ORC	606:613	arg1	degradable					634:643	degradable	634:643	degradable	634:643	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	4	68	theme	O-CS/ORC	606:613	arg1	gauze					625:629	The N, O-CS/ORC composite gauze	599:629	gauze	625:629	The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria.
30599998	5	69	theme	notable	770:776	arg1	efficacy					789:796	the notable hemostatic efficacy	766:796	the notable hemostatic efficacy of the N, O-CS/ORC composite gauze	766:831	Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models.
30599998	3	70	theme	chitosan/oxidized	515:531	arg1	gauze					579:583	the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze	492:583	gauze	579:583	In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared.
30599998	7	71	theme	O-CS/ORC	1192:1199	arg1	gauze					1211:1215	O-CS/ORC composite gauze	1192:1215	O-CS/ORC composite gauze	1192:1215	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30599998	7	71	theme	O-CS/ORC	1192:1199	arg1	N					1189:1189	N	1189:1189	N	1189:1189	In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery.
30797366	0	0	theme	prebiotic	77:85	arg1	activity					87:94	potential prebiotic activity	67:94	potential prebiotic activity	67:94	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	3	1	theme	carbograph	525:534	arg1	cartridges					536:545	carbograph cartridges	525:545	carbograph cartridges	525:545	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	0	2	theme	potential	67:75	arg1	activity					87:94	potential prebiotic activity	67:94	potential prebiotic activity	67:94	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	0	3	from	impact	4:9	arg1	composition					51:61	chemical composition	42:61	chemical composition	42:61	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	0	3	from	impact	4:9	arg1	activity					87:94	potential prebiotic activity	67:94	potential prebiotic activity	67:94	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	6	4	theme	well-known	944:953	arg1	plantarum					1027:1035	Lactobacillus plantarum	1013:1035	Lactobacillus plantarum	1013:1035	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	6	4	theme	well-known	944:953	arg1	acidophilus					997:1007	Lactobacillus acidophilus	983:1007	Lactobacillus acidophilus	983:1007	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	6	4	theme	well-known	944:953	arg1	bacteria					965:972	well-known probiotic bacteria	944:972	well-known probiotic bacteria	944:972	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	5	5	from	arabinose	790:798	arg1	rich					782:785	rich	782:785	rich	782:785	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	5	6	theme	Complex	709:715	arg1	oligosaccharides					717:732	Complex oligosaccharides	709:732	Complex oligosaccharides	709:732	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	6	7	theme	functional	890:899	arg1	ingredients					901:911	potential functional ingredients	880:911	potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum,	880:1036	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	3	8	dep	approach	506:513	arg1	C-18					516:519	C-18	516:519	C-18	516:519	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	3	8	dep	approach	506:513	arg1	cartridges					536:545	carbograph cartridges	525:545	carbograph cartridges	525:545	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	5	9	theme	rich	782:785	arg1	oligosaccharides					765:780	neutral oligosaccharides	757:780	neutral oligosaccharides rich in arabinose and glucose	757:810	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	6	10	theme	potential	880:888	arg1	ingredients					901:911	potential functional ingredients	880:911	potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum,	880:1036	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	4	11	theme	Chemical	623:630	arg1	characterization					632:647	Chemical characterization	623:647	Chemical characterization of the fractions	623:664	Chemical characterization of the fractions was performed using a UPLC-ESI-MSn method.
30797366	0	12	theme	oligosaccharidic	109:124	arg1	fractions					126:134	different oligosaccharidic fractions	99:134	different oligosaccharidic fractions	99:134	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	5	13	from	rich	782:785	arg1	glucose					804:810	glucose	804:810	glucose	804:810	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	5	13	from	rich	782:785	arg1	arabinose					790:798	arabinose	790:798	arabinose	790:798	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	2	14	theme	pomace	413:418	arg1	distillation					420:431	grape pomace distillation	407:431	grape pomace distillation in order to valorize this by-product	407:468	Different oligosaccharidic fractions have been extracted both before and after grape pomace distillation in order to valorize this by-product.
30797366	0	15	theme	different	99:107	arg1	fractions					126:134	different oligosaccharidic fractions	99:134	different oligosaccharidic fractions	99:134	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	2	16	theme	grape	407:411	arg1	distillation					420:431	grape pomace distillation	407:431	grape pomace distillation in order to valorize this by-product	407:468	Different oligosaccharidic fractions have been extracted both before and after grape pomace distillation in order to valorize this by-product.
30797366	7	17	theme	potential	1183:1191	arg1	activity					1203:1210	potential prebiotic activity	1183:1210	potential prebiotic activity mainly towards L. acidophilus	1183:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	6	18	theme	different	857:865	arg1	fractions					867:875	the different fractions	853:875	the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum,	853:1036	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	7	19	theme	functional	1155:1164	arg1	"					1176:1176	a novel "functional ingredient"	1146:1176	a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus	1146:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	7	20	theme	prebiotic	1193:1201	arg1	activity					1203:1210	potential prebiotic activity	1183:1210	potential prebiotic activity mainly towards L. acidophilus	1183:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	3	21	theme	oligosaccharidic	595:610	arg1	compounds					612:620	the oligosaccharidic compounds	591:620	the oligosaccharidic compounds	591:620	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	0	22	theme	distillation	14:25	arg1	process					27:33	distillation process	14:33	distillation process	14:33	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	1	23	theme	oligosaccharides	281:296	arg1	composition					250:260	composition	250:260	composition	250:260	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	1	23	theme	oligosaccharides	281:296	arg1	bioactivity					266:276	bioactivity	266:276	bioactivity	266:276	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	7	24	with	"	1176:1176	arg1	activity					1203:1210	potential prebiotic activity	1183:1210	potential prebiotic activity mainly towards L. acidophilus	1183:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	3	25	theme	solid-phase	483:493	arg1	approach					506:513	A multistep solid-phase extraction approach	471:513	A multistep solid-phase extraction approach (C-18 and carbograph cartridges)	471:546	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	0	26	theme	fractions	126:134	arg1	composition					51:61	chemical composition	42:61	chemical composition	42:61	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	0	26	theme	fractions	126:134	arg1	activity					87:94	potential prebiotic activity	67:94	potential prebiotic activity	67:94	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	0	27	dep	composition	51:61	arg1	the					38:40	the	38:40	the	38:40	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	3	28	theme	extraction	495:504	arg1	approach					506:513	A multistep solid-phase extraction approach	471:513	A multistep solid-phase extraction approach (C-18 and carbograph cartridges)	471:546	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	0	29	theme	grape	151:155	arg1	seeds					157:161	grape seeds	151:161	grape seeds	151:161	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	4	30	theme	UPLC-ESI-MSn	688:699	arg1	method					701:706	a UPLC-ESI-MSn method	686:706	a UPLC-ESI-MSn method	686:706	Chemical characterization of the fractions was performed using a UPLC-ESI-MSn method.
30797366	1	31	theme	distillation	207:218	arg1	able					231:234	able	231:234	able	231:234	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	1	31	theme	distillation	207:218	arg1	process					220:226	the distillation process	203:226	the distillation process	203:226	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	0	32	theme	process	27:33	arg1	impact					4:9	The impact	0:9	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions	0:134	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	7	33	theme	ingredient	1166:1175	arg1	"					1176:1176	a novel "functional ingredient"	1146:1176	a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus	1146:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	6	34	theme	probiotic	955:963	arg1	plantarum					1027:1035	Lactobacillus plantarum	1013:1035	Lactobacillus plantarum	1013:1035	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	6	34	theme	probiotic	955:963	arg1	acidophilus					997:1007	Lactobacillus acidophilus	983:1007	Lactobacillus acidophilus	983:1007	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	6	34	theme	probiotic	955:963	arg1	bacteria					965:972	well-known probiotic bacteria	944:972	well-known probiotic bacteria	944:972	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	6	35	with	ingredients	901:911	arg1	activity					928:935	prebiotic activity	918:935	prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum,	918:1036	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	3	36	theme	multistep	473:481	arg1	approach					506:513	A multistep solid-phase extraction approach	471:513	A multistep solid-phase extraction approach (C-18 and carbograph cartridges)	471:546	A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds.
30797366	0	37	theme	chemical	42:49	arg1	composition					51:61	chemical composition	42:61	chemical composition	42:61	The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.
30797366	1	38	theme	grape	315:319	arg1	seeds					321:325	the grape seeds	311:325	the grape seeds	311:325	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	7	39	theme	novel	1148:1152	arg1	"					1176:1176	a novel "functional ingredient"	1146:1176	a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus	1146:1240	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	5	40	from	glucose	804:810	arg1	rich					782:785	rich	782:785	rich	782:785	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	2	41	theme	oligosaccharidic	338:353	arg1	fractions					355:363	Different oligosaccharidic fractions	328:363	Different oligosaccharidic fractions	328:363	Different oligosaccharidic fractions have been extracted both before and after grape pomace distillation in order to valorize this by-product.
30797366	5	42	theme	neutral	757:763	arg1	oligosaccharides					765:780	neutral oligosaccharides	757:780	neutral oligosaccharides rich in arabinose and glucose	757:810	Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose.
30797366	6	43	theme	prebiotic	918:926	arg1	activity					928:935	prebiotic activity	918:935	prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum,	918:1036	Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated.
30797366	2	44	theme	Different	328:336	arg1	fractions					355:363	Different oligosaccharidic fractions	328:363	Different oligosaccharidic fractions	328:363	Different oligosaccharidic fractions have been extracted both before and after grape pomace distillation in order to valorize this by-product.
30797366	1	45	dep	composition	250:260	arg1	the					246:248	the	246:248	the	246:248	The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds.
30797366	7	46	theme	oligosaccharidic	1092:1107	arg1	fractions					1109:1117	oligosaccharidic fractions	1092:1117	oligosaccharidic fractions obtained	1092:1126	Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
30797366	4	47	theme	fractions	656:664	arg1	characterization					632:647	Chemical characterization	623:647	Chemical characterization of the fractions	623:664	Chemical characterization of the fractions was performed using a UPLC-ESI-MSn method.
31619635	4	0	theme	cecal	835:839	arg1	parameters					841:850	lower cecal parameters	829:850	lower cecal parameters	829:850	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	5	1	theme	significant	973:983	arg1	effect					985:990	a significant effect	971:990	a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters	971:1089	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	0	2	theme	Tissue	154:159	arg1	Parameters					118:127	the Physiological Parameters	100:127	the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile	100:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	5	3	theme	SCFA	1048:1051	arg1	contents					1053:1060	SCFA contents	1048:1060	SCFA contents	1048:1060	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	4	4	theme	lower	829:833	arg1	parameters					841:850	lower cecal parameters	829:850	lower cecal parameters	829:850	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	0	5	theme	Adipose	146:152	arg1	Tissue					154:159	Adipose Tissue	146:159	Adipose Tissue	146:159	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	6	theme	cecal	547:551	arg1	parameters					553:562	significantly higher cecal parameters	526:562	significantly higher cecal parameters	526:562	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	5	7	theme	cecal	1030:1034	arg1	parameters					1036:1045	cecal parameters	1030:1045	cecal parameters	1030:1045	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	3	8	theme	other	714:718	arg1	groups					722:727	other 3 groups	714:727	other 3 groups	714:727	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	4	9	theme	S-Rf	801:804	arg1	groups					806:811	the CON, S-Wh and S-Rf groups	783:811	the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents	783:868	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	0	10	theme	Fasting	165:171	arg1	Profile					185:191	Fasting Serum Lipid Profile	165:191	Fasting Serum Lipid Profile	165:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	11	theme	higher	540:545	arg1	parameters					553:562	significantly higher cecal parameters	526:562	significantly higher cecal parameters	526:562	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	3	12	theme	S-Wh	608:611	arg1	group					613:617	S-Wh group	608:617	S-Wh group	608:617	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	0	13	theme	Important	62:70	arg1	Correlations					72:83	Important Correlations	62:83	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.	0:192	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	14	theme	final	482:486	arg1	weight					493:498	final body weight	482:498	final body weight	482:498	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	14	theme	final	482:486	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	5	15	theme	physiological	1066:1078	arg1	parameters					1080:1089	physiological parameters	1066:1089	physiological parameters	1066:1089	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	1	16	theme	α-corn	281:286	arg1	diets					301:305	α-corn starch (CON) diets	281:305	α-corn starch (CON) diets	281:305	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	17	theme	Lipid	179:183	arg1	Profile					185:191	Fasting Serum Lipid Profile	165:191	Fasting Serum Lipid Profile	165:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	5	18	from	effect	985:990	arg1	parameters					1036:1045	cecal parameters	1030:1045	cecal parameters	1030:1045	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	18	from	effect	985:990	arg1	contents					1053:1060	SCFA contents	1048:1060	SCFA contents	1048:1060	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	18	from	effect	985:990	arg1	parameters					1080:1089	physiological parameters	1066:1089	physiological parameters	1066:1089	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	19	from	compositions	891:902	arg1	HAS					907:909	HAS	907:909	HAS	907:909	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	1	20	theme	starch	288:293	arg1	diets					301:305	α-corn starch (CON) diets	281:305	α-corn starch (CON) diets	281:305	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	21	theme	Serum	173:177	arg1	Profile					185:191	Fasting Serum Lipid Profile	165:191	Fasting Serum Lipid Profile	165:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	22	theme	chain	574:578	arg1	SCFA					592:595	SCFA	592:595	SCFA	592:595	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	22	theme	chain	574:578	arg1	acid					586:589	chain fatty acid	574:589	short chain fatty acid (SCFA) contents	568:605	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	3	23	theme	lipid	685:689	arg1	parameters					691:700	serum lipid parameters	679:700	serum lipid parameters	679:700	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	5	24	theme	distinct	942:949	arg1	clusters					951:958	3 distinct clusters	940:958	3 distinct clusters	940:958	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	25	theme	microbial	1005:1013	arg1	composition					1015:1025	the cecal microbial composition	995:1025	the cecal microbial composition	995:1025	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	2	26	theme	serum	501:505	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	26	theme	serum	501:505	arg1	profile					513:519	serum lipid profile	501:519	serum lipid profile	501:519	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	27	theme	short	568:572	arg1	contents					598:605	short chain fatty acid (SCFA) contents	568:605	short chain fatty acid (SCFA) contents	568:605	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	28	theme	feed	469:472	arg1	weight					493:498	final body weight	482:498	final body weight	482:498	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	28	theme	feed	469:472	arg1	profile					513:519	serum lipid profile	501:519	serum lipid profile	501:519	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	28	theme	feed	469:472	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	0	29	theme	In	0:1	arg1	Fermentation					16:27	In Vivo Colonic Fermentation	0:27	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.	0:192	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	1	30	theme	whole	226:230	arg1	sorghum					238:244	whole grain sorghum	226:244	whole grain sorghum (S-Wh)	226:251	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	30	theme	whole	226:230	arg1	S-Wh					247:250	S-Wh	247:250	S-Wh	247:250	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	31	theme	Profile	185:191	arg1	Parameters					118:127	the Physiological Parameters	100:127	the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile	100:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	1	32	theme	physiological	358:370	arg1	effects					372:378	the physiological effects	354:378	the physiological effects of resistant starch inclusion in the diet from grains	354:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	2	33	theme	body	488:491	arg1	weight					493:498	final body weight	482:498	final body weight	482:498	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	33	theme	body	488:491	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	1	34	theme	grain	232:236	arg1	sorghum					238:244	whole grain sorghum	226:244	whole grain sorghum (S-Wh)	226:251	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	34	theme	grain	232:236	arg1	S-Wh					247:250	S-Wh	247:250	S-Wh	247:250	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	35	theme	Physiological	104:116	arg1	Parameters					118:127	the Physiological Parameters	100:127	the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile	100:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	5	36	theme	microbial	881:889	arg1	compositions					891:902	The cecal microbial compositions	871:902	The cecal microbial compositions in HAS	871:909	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	36	theme	microbial	881:889	arg1	CON/S-Rf					921:928	CON/S-Rf	921:928	CON/S-Rf	921:928	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	36	theme	microbial	881:889	arg1	S-Wh					912:915	S-Wh	912:915	S-Wh	912:915	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	0	37	theme	Colonic	8:14	arg1	Fermentation					16:27	In Vivo Colonic Fermentation	0:27	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.	0:192	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	38	theme	acid	586:589	arg1	contents					598:605	short chain fatty acid (SCFA) contents	568:605	short chain fatty acid (SCFA) contents	568:605	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	1	39	theme	CON	296:298	arg1	diets					301:305	α-corn starch (CON) diets	281:305	α-corn starch (CON) diets	281:305	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	40	dep	bicolor	49:55	arg1	L.					57:58	Sorghum bicolor L.	41:58	Sorghum bicolor L.	41:58	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	0	41	theme	Sorghum	32:38	arg1	Fermentation					16:27	In Vivo Colonic Fermentation	0:27	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.	0:192	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	4	42	theme	S-Wh	792:795	arg1	groups					806:811	the CON, S-Wh and S-Rf groups	783:811	the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents	783:868	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	1	43	dep	starch	212:217	arg1	HAS					220:222	HAS	220:222	HAS	220:222	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	2	44	theme	fatty	580:584	arg1	SCFA					592:595	SCFA	592:595	SCFA	592:595	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	44	theme	fatty	580:584	arg1	acid					586:589	chain fatty acid	574:589	short chain fatty acid (SCFA) contents	568:605	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	4	45	from	groups	806:811	arg1	prominent					770:778	prominent	770:778	prominent	770:778	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	5	46	theme	composition	1015:1025	arg1	effect					985:990	a significant effect	971:990	a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters	971:1089	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	0	47	theme	Sorghum	41:47	arg1	Sorghum					32:38	Sorghum	32:38	Sorghum (Sorghum bicolor L.)	32:59	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	0	47	theme	Sorghum	41:47	arg1	bicolor					49:55	Sorghum bicolor L.	41:58	Sorghum bicolor L.	41:58	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	3	48	theme	feed	663:666	arg1	intake					668:673	feed intake	663:673	feed intake	663:673	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	3	49	theme	higher	643:648	arg1	weight					655:660	significantly higher body weight	629:660	significantly higher body weight	629:660	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	5	50	theme	cecal	875:879	arg1	compositions					891:902	The cecal microbial compositions	871:902	The cecal microbial compositions in HAS	871:909	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	50	theme	cecal	875:879	arg1	CON/S-Rf					921:928	CON/S-Rf	921:928	CON/S-Rf	921:928	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	5	50	theme	cecal	875:879	arg1	S-Wh					912:915	S-Wh	912:915	S-Wh	912:915	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
31619635	1	51	theme	resistant	383:391	arg1	starch					393:398	resistant starch	383:398	resistant starch inclusion	383:408	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	4	52	theme	SCFA	856:859	arg1	contents					861:868	SCFA contents	856:868	SCFA contents	856:868	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	3	53	theme	body	650:653	arg1	weight					655:660	significantly higher body weight	629:660	significantly higher body weight	629:660	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	0	54	theme	Cecum	132:136	arg1	Parameters					118:127	the Physiological Parameters	100:127	the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile	100:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	2	55	theme	lipid	507:511	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	55	theme	lipid	507:511	arg1	profile					513:519	serum lipid profile	501:519	serum lipid profile	501:519	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	1	56	theme	starch	393:398	arg1	inclusion					400:408	resistant starch inclusion	383:408	resistant starch inclusion	383:408	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	57	from	effects	372:378	arg1	grains					427:432	grains	427:432	grains	427:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	57	from	effects	372:378	arg1	diet					417:420	the diet	413:420	the diet from grains	413:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	58	theme	refined	254:260	arg1	S-Rf					271:274	S-Rf	271:274	S-Rf	271:274	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	58	theme	refined	254:260	arg1	sorghum					262:268	refined sorghum	254:268	refined sorghum (S-Rf)	254:275	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	2	59	theme	lower	463:467	arg1	weight					493:498	final body weight	482:498	final body weight	482:498	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	59	theme	lower	463:467	arg1	profile					513:519	serum lipid profile	501:519	serum lipid profile	501:519	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	2	59	theme	lower	463:467	arg1	intake					474:479	significantly lower feed intake	449:479	significantly lower feed intake	449:479	HAS exhibited significantly lower feed intake, final body weight, serum lipid profile with significantly higher cecal parameters and short chain fatty acid (SCFA) contents.
31619635	4	60	from	prominent	770:778	arg1	groups					806:811	the CON, S-Wh and S-Rf groups	783:811	the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents	783:868	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	0	61	dep	Fermentation	16:27	arg1	Correlations					72:83	Important Correlations	62:83	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.	0:192	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	1	62	theme	inclusion	400:408	arg1	effects					372:378	the physiological effects	354:378	the physiological effects of resistant starch inclusion in the diet from grains	354:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	63	theme	High	194:197	arg1	starch					212:217	High amylose corn starch	194:217	High amylose corn starch (HAS)	194:223	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	64	theme	Liver	139:143	arg1	Parameters					118:127	the Physiological Parameters	100:127	the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile	100:191	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	4	65	theme	Cecal	730:734	arg1	fermentation					736:747	Cecal fermentation	730:747	Cecal fermentation	730:747	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	1	66	theme	amylose	199:205	arg1	starch					212:217	High amylose corn starch	194:217	High amylose corn starch (HAS)	194:223	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	4	67	theme	CON	787:789	arg1	groups					806:811	the CON, S-Wh and S-Rf groups	783:811	the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents	783:868	Cecal fermentation was not seemed to be prominent in the CON, S-Wh and S-Rf groups with respect to lower cecal parameters and SCFA contents.
31619635	1	68	from	grains	427:432	arg1	diet					417:420	the diet	413:420	the diet from grains	413:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	68	from	grains	427:432	arg1	effects					372:378	the physiological effects	354:378	the physiological effects of resistant starch inclusion in the diet from grains	354:432	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	1	69	theme	corn	207:210	arg1	starch					212:217	High amylose corn starch	194:217	High amylose corn starch (HAS)	194:223	High amylose corn starch (HAS), whole grain sorghum (S-Wh), refined sorghum (S-Rf) and α-corn starch (CON) diets were fed to animals for 1 mo aiming to examine the physiological effects of resistant starch inclusion in the diet from grains.
31619635	0	70	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo Colonic Fermentation of Sorghum (Sorghum bicolor L.): Important Correlations Observed among the Physiological Parameters of Cecum, Liver, Adipose Tissue and Fasting Serum Lipid Profile.
31619635	3	71	theme	serum	679:683	arg1	parameters					691:700	serum lipid parameters	679:700	serum lipid parameters	679:700	S-Wh group exhibited significantly higher body weight, feed intake and serum lipid parameters compared to other 3 groups.
31619635	5	72	theme	cecal	999:1003	arg1	composition					1015:1025	the cecal microbial composition	995:1025	the cecal microbial composition	995:1025	The cecal microbial compositions in HAS, S-Wh and CON/S-Rf exhibited 3 distinct clusters suggesting a significant effect of the cecal microbial composition on cecal parameters, SCFA contents and physiological parameters.
30976784	5	0	theme	activities	846:855	arg1	Comparison					815:824	Comparison	815:824	Comparison	815:824	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	1	1	theme	N-linked	160:167	arg1	oligosaccharides					169:184	the N-linked oligosaccharides	156:184	the N-linked oligosaccharides of newly synthesized glycoproteins	156:219	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	6	2	theme	in	1186:1187	arg1	modeling					1196:1203	in silico modeling	1186:1203	in silico modeling	1186:1203	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	5	3	from	microsomes	863:872	arg1	Comparison					815:824	Comparison	815:824	Comparison	815:824	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	1	4	gly	glycoproteins	207:219	arg1	glycoproteins					207:219	newly synthesized glycoproteins	189:219	newly synthesized glycoproteins	189:219	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	5	5	theme	selective	972:980	arg1	inhibitor					982:990	a selective inhibitor	970:990	a selective inhibitor of ER α-glucosidase I	970:1012	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	5	5	theme	selective	972:980	arg1	3,7a-diepi-alexine					943:960	3,7a-diepi-alexine	943:960	3,7a-diepi-alexine	943:960	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	4	6	theme	oligosaccharide	714:728	arg1	processing					730:739	oligosaccharide processing	714:739	oligosaccharide processing	714:739	Using ER microsomes to recapitulate authentic protein N-glycosylation and oligosaccharide processing, we identify five iminosugars that selectively inhibit N-glycan trimming.
30976784	1	7	theme	endoplasmic	67:77	arg1	reticulum					79:87	The endoplasmic reticulum	63:87	The endoplasmic reticulum (ER)	63:92	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	1	7	theme	endoplasmic	67:77	arg1	ER					90:91	ER	90:91	ER	90:91	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	7	8	theme	promising	1374:1382	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	8	theme	promising	1374:1382	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	8	theme	promising	1374:1382	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	1	9	gly	glycoprotein	268:279	arg1	glycoprotein					268:279	glycoprotein quality control	268:295	glycoprotein quality control	268:295	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	2	10	theme	structural	311:320	arg1	mimetics					322:329	structural mimetics	311:329	structural mimetics	311:329	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	2	11	theme	molecular	448:456	arg1	basis					458:462	a molecular basis	446:462	a molecular basis for their therapeutic applications	446:497	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	5	12	from	effects	888:894	arg1	α-glucosidase					911:923	purified ER α-glucosidase II	899:926	purified ER α-glucosidase II	899:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	5	13	theme	ER	860:861	arg1	microsomes					863:872	ER microsomes	860:872	ER microsomes against their effects on purified ER α-glucosidase II	860:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	6	14	theme	enhanced	1248:1255	arg1	activity					1257:1264	this enhanced activity	1243:1264	this enhanced activity	1243:1264	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	6	15	theme	compounds	1168:1176	arg1	effective					1149:1157	effective	1149:1157	effective	1149:1157	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	6	15	theme	compounds	1168:1176	arg1	1,4-dideoxy-1,4-imino-D-arabinitol					1096:1129	1,4-dideoxy-1,4-imino-D-arabinitol	1096:1129	1,4-dideoxy-1,4-imino-D-arabinitol (DAB)	1096:1135	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	3	16	from	effects	534:540	arg1	actions					580:586	the actions	576:586	the actions of ER luminal α-glucosidase I and α-glucosidase II	576:637	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	3	17	theme	α-glucosidase	602:614	arg1	I					616:616	ER luminal α-glucosidase I	591:616	ER luminal α-glucosidase I	591:616	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	1	18	theme	synthesized	195:205	arg1	glycoproteins					207:219	newly synthesized glycoproteins	189:219	newly synthesized glycoproteins	189:219	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	7	19	theme	pyrrolizidines	1323:1336	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	19	theme	pyrrolizidines	1323:1336	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	19	theme	pyrrolizidines	1323:1336	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	3	20	theme	I	616:616	arg1	actions					580:586	the actions	576:586	the actions of ER luminal α-glucosidase I and α-glucosidase II	576:637	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	1	21	theme	glycoproteins	207:219	arg1	oligosaccharides					169:184	the N-linked oligosaccharides	156:184	the N-linked oligosaccharides of newly synthesized glycoproteins	156:219	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	5	22	theme	purified	899:906	arg1	α-glucosidase					911:923	purified ER α-glucosidase II	899:926	purified ER α-glucosidase II	899:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	1	23	link	N-linked	160:167	arg1	oligosaccharides					169:184	the N-linked oligosaccharides	156:184	the N-linked oligosaccharides of newly synthesized glycoproteins	156:219	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	6	24	dep	in	1186:1187	arg1	silico					1189:1194	silico	1189:1194	silico	1189:1194	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	1	25	dep	process	148:154	arg1	facilitate					233:242	facilitate	233:242	facilitate polypeptide folding and glycoprotein quality control	233:295	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	2	26	theme	therapeutic	474:484	arg1	applications					486:497	their therapeutic applications	468:497	their therapeutic applications	468:497	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	5	27	theme	ER	908:909	arg1	α-glucosidase					911:923	purified ER α-glucosidase II	899:926	purified ER α-glucosidase II	899:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	3	28	theme	panel	547:551	arg1	effects					534:540	the effects	530:540	the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II	530:637	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	5	29	from	Comparison	815:824	arg1	microsomes					863:872	ER microsomes	860:872	ER microsomes against their effects on purified ER α-glucosidase II	860:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	3	30	theme	luminal	594:600	arg1	I					616:616	ER luminal α-glucosidase I	591:616	ER luminal α-glucosidase I	591:616	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	0	31	theme	α-glucosidase	37:49	arg1	inhibitors					51:60	ER α-glucosidase inhibitors	34:60	ER α-glucosidase inhibitors	34:60	Characterizing the selectivity of ER α-glucosidase inhibitors.
30976784	5	32	theme	α-glucosidase	998:1010	arg1	inhibitor					982:990	a selective inhibitor	970:990	a selective inhibitor of ER α-glucosidase I	970:1012	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	5	32	theme	α-glucosidase	998:1010	arg1	3,7a-diepi-alexine					943:960	3,7a-diepi-alexine	943:960	3,7a-diepi-alexine	943:960	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	3	33	theme	II	636:637	arg1	actions					580:586	the actions	576:586	the actions of ER luminal α-glucosidase I and α-glucosidase II	576:637	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	7	34	theme	iminosugar	1404:1413	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	34	theme	iminosugar	1404:1413	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	34	theme	iminosugar	1404:1413	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	35	theme	"	1402:1402	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	35	theme	"	1402:1402	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	35	theme	"	1402:1402	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	0	36	theme	ER	34:35	arg1	inhibitors					51:60	ER α-glucosidase inhibitors	34:60	ER α-glucosidase inhibitors	34:60	Characterizing the selectivity of ER α-glucosidase inhibitors.
30976784	6	37	theme	iminosugars	1032:1042	arg1	all					1044:1046	The other active iminosugars all	1015:1046	The other active iminosugars all	1015:1046	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	7	38	dep	substituted	1311:1321	arg1	C-3					1307:1309	C-3	1307:1309	C-3	1307:1309	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	1	39	theme	polypeptide	244:254	arg1	folding					256:262	polypeptide folding	244:262	polypeptide folding	244:262	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	1	40	contain	contains	94:101	arg1	reticulum					79:87	The endoplasmic reticulum	63:87	The endoplasmic reticulum (ER)	63:92	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	1	40	contain	contains	94:101	arg2	α-glucosidases					108:121	α-glucosidases	108:121	α-glucosidases	108:121	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	1	40	contain	contains	94:101	arg1	ER					90:91	ER	90:91	ER	90:91	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	1	40	contain	contains	94:101	arg2	α-mannosidases					127:140	α-mannosidases	127:140	α-mannosidases	127:140	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	7	41	theme	substituted	1311:1321	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	41	theme	substituted	1311:1321	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	41	theme	substituted	1311:1321	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	5	42	theme	ER	995:996	arg1	α-glucosidase					998:1010	ER α-glucosidase I	995:1012	ER α-glucosidase I	995:1012	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	0	43	theme	inhibitors	51:60	arg1	selectivity					19:29	the selectivity	15:29	the selectivity of ER α-glucosidase inhibitors	15:60	Characterizing the selectivity of ER α-glucosidase inhibitors.
30976784	6	44	theme	other	1019:1023	arg1	all					1044:1046	The other active iminosugars all	1015:1046	The other active iminosugars all	1015:1046	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	5	45	from	activities	846:855	arg1	microsomes					863:872	ER microsomes	860:872	ER microsomes against their effects on purified ER α-glucosidase II	860:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	3	46	theme	α-glucosidase	622:634	arg1	II					636:637	α-glucosidase II	622:637	α-glucosidase II	622:637	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	2	47	theme	localized	377:385	arg1	α-glycosidases					387:400	these ER localized α-glycosidases	368:400	these ER localized α-glycosidases	368:400	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	2	48	theme	ER	374:375	arg1	α-glycosidases					387:400	these ER localized α-glycosidases	368:400	these ER localized α-glycosidases	368:400	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	3	49	theme	ER	591:592	arg1	I					616:616	ER luminal α-glucosidase I	591:616	ER luminal α-glucosidase I	591:616	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	4	50	theme	ER	646:647	arg1	microsomes					649:658	ER microsomes	646:658	ER microsomes	646:658	Using ER microsomes to recapitulate authentic protein N-glycosylation and oligosaccharide processing, we identify five iminosugars that selectively inhibit N-glycan trimming.
30976784	4	51	theme	N-glycan	796:803	arg1	trimming					805:812	N-glycan trimming	796:812	N-glycan trimming	796:812	Using ER microsomes to recapitulate authentic protein N-glycosylation and oligosaccharide processing, we identify five iminosugars that selectively inhibit N-glycan trimming.
30976784	3	52	theme	iminosugars	561:571	arg1	panel					547:551	a panel	545:551	a panel of nine iminosugars	545:571	In this study, we investigate the effects of a panel of nine iminosugars on the actions of ER luminal α-glucosidase I and α-glucosidase II.
30976784	2	53	dep	inhibit	360:366	arg1	preventing					403:412	preventing	403:412	preventing N-glycan trimming	403:430	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	2	53	dep	inhibit	360:366	arg1	providing					436:444	providing	436:444	providing a molecular basis for their therapeutic applications	436:497	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	4	54	theme	protein	686:692	arg1	N-glycosylation					694:708	authentic protein N-glycosylation	676:708	authentic protein N-glycosylation	676:708	Using ER microsomes to recapitulate authentic protein N-glycosylation and oligosaccharide processing, we identify five iminosugars that selectively inhibit N-glycan trimming.
30976784	4	55	theme	authentic	676:684	arg1	N-glycosylation					694:708	authentic protein N-glycosylation	676:708	authentic protein N-glycosylation	676:708	Using ER microsomes to recapitulate authentic protein N-glycosylation and oligosaccharide processing, we identify five iminosugars that selectively inhibit N-glycan trimming.
30976784	1	56	theme	glycoprotein	268:279	arg1	control					289:295	glycoprotein quality control	268:295	glycoprotein quality control	268:295	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
30976784	5	57	theme	inhibitory	835:844	arg1	activities					846:855	their inhibitory activities	829:855	their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II	829:926	Comparison of their inhibitory activities in ER microsomes against their effects on purified ER α-glucosidase II, suggests that 3,7a-diepi-alexine acts as a selective inhibitor of ER α-glucosidase I.
30976784	6	58	theme	molecular	1223:1231	arg1	basis					1233:1237	the molecular basis	1219:1237	the molecular basis for this enhanced activity	1219:1264	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	7	59	theme	second-generation	1385:1401	arg1	inhibitors					1415:1424	promising "second-generation" iminosugar inhibitors	1374:1424	promising "second-generation" iminosugar inhibitors	1374:1424	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	59	theme	second-generation	1385:1401	arg1	3,7a-diepi-alexine					1352:1369	3,7a-diepi-alexine	1352:1369	3,7a-diepi-alexine	1352:1369	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	7	59	theme	second-generation	1385:1401	arg1	casuarine					1338:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	the C-3 substituted pyrrolizidines casuarine	1303:1346	Taken together, our work identifies the C-3 substituted pyrrolizidines casuarine and 3,7a-diepi-alexine as promising "second-generation" iminosugar inhibitors.
30976784	2	60	theme	N-glycan	414:421	arg1	trimming					423:430	N-glycan trimming	414:430	N-glycan trimming	414:430	By acting as structural mimetics, iminosugars can selectively inhibit these ER localized α-glycosidases, preventing N-glycan trimming and providing a molecular basis for their therapeutic applications.
30976784	6	61	theme	active	1025:1030	arg1	all					1044:1046	The other active iminosugars all	1015:1046	The other active iminosugars all	1015:1046	The other active iminosugars all inhibit α-glucosidase II and, having identified 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) as the most effective of these compounds, we use in silico modeling to understand the molecular basis for this enhanced activity.
30976784	1	62	theme	quality	281:287	arg1	control					289:295	glycoprotein quality control	268:295	glycoprotein quality control	268:295	The endoplasmic reticulum (ER) contains both α-glucosidases and α-mannosidases which process the N-linked oligosaccharides of newly synthesized glycoproteins and thereby facilitate polypeptide folding and glycoprotein quality control.
29976057	0	0	theme	Starch	78:83	arg1	Hydrolysis					85:94	Starch Hydrolysis	78:94	Starch Hydrolysis	78:94	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	4	1	theme	thermal	821:827	arg1	stability					829:837	higher thermal stability	814:837	higher thermal stability of the composite	814:854	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	2	theme	enzyme	359:364	arg1	immobilization					366:379	enzyme immobilization	359:379	enzyme immobilization within the pores of a rigid magnetic matrix	359:423	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	4	3	from	rate	669:672	arg1	level					622:626	the level	618:626	the level of 10 units/mg	618:641	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	4	theme	amylase	558:564	arg1	composite					573:581	the amylase@ferria composite	554:581	the amylase@ferria composite	554:581	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	5	5	theme	excellent	882:890	arg1	stability					892:900	excellent stability	882:900	excellent stability	882:900	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	3	6	theme	enzyme	470:475	arg1	fractions					482:490	enzyme mass fractions	470:490	enzyme mass fractions less than 10 wt %	470:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	4	7	theme	amylase	733:739	arg1	ferria					741:746	amylase@ferria	733:746	amylase@ferria	733:746	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	6	8	theme	reusable	1099:1106	arg1	material					1043:1050	The material	1039:1050	The material	1039:1050	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	8	theme	reusable	1099:1106	arg1	catalyst					1108:1115	a magnetically separable reusable catalyst	1074:1115	a magnetically separable reusable catalyst	1074:1115	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	4	9	theme	units/mg	634:641	arg1	level					622:626	the level	618:626	the level of 10 units/mg	618:641	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	10	link	magnetite-derived	261:277	arg1	matrix					287:292	a magnetite-derived xerogel matrix	259:292	a magnetite-derived xerogel matrix	259:292	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	5	11	theme	Entrapped	857:865	arg1	amylase					867:873	Entrapped amylase	857:873	Entrapped amylase	857:873	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	5	12	theme	free	988:991	arg1	enzyme					993:998	free enzyme	988:998	free enzyme	988:998	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	6	13	theme	separable	1089:1097	arg1	material					1043:1050	The material	1039:1050	The material	1039:1050	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	13	theme	separable	1089:1097	arg1	catalyst					1108:1115	a magnetically separable reusable catalyst	1074:1115	a magnetically separable reusable catalyst	1074:1115	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	4	14	theme	comparable	674:683	arg1	rate					669:672	the starch hydrolysis rate	647:672	the starch hydrolysis rate comparable to free enzyme	647:698	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	15	theme	@	565:565	arg1	composite					573:581	the amylase@ferria composite	554:581	the amylase@ferria composite	554:581	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	6	16	theme	catalytic	1122:1130	arg1	material					1043:1050	The material	1039:1050	The material	1039:1050	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	16	theme	catalytic	1122:1130	arg1	coating					1140:1146	a catalytic ceramic coating	1120:1146	a catalytic ceramic coating with at least 10 cycles of use	1120:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	2	17	theme	matrix	418:423	arg1	immobilization					366:379	enzyme immobilization	359:379	enzyme immobilization within the pores of a rigid magnetic matrix	359:423	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	6	18	theme	use	1175:1177	arg1	cycles					1165:1170	at least 10 cycles	1153:1170	at least 10 cycles of use	1153:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	1	19	theme	new	150:152	arg1	class					154:158	a new class	148:158	a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	148:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	5	20	from	solution	972:979	arg1	days					944:947	21 days	941:947	21 days of storage in a buffer solution	941:979	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	2	21	theme	xerogel	279:285	arg1	matrix					287:292	a magnetite-derived xerogel matrix	259:292	a magnetite-derived xerogel matrix	259:292	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	1	22	theme	class	154:158	arg1	nanocomposites					190:203	a new class recyclable magnetic catalytic nanocomposites	148:203	a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	148:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	0	23	theme	@	9:9	arg1	Ferria					10:15	α-Amylase@Ferria	0:15	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.	0:95	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	5	24	from	storage	952:958	arg1	solution					972:979	a buffer solution	963:979	a buffer solution	963:979	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	4	25	theme	free	786:789	arg1	amylase					791:797	free amylase	786:797	free amylase as a result of higher thermal stability of the composite	786:854	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	26	theme	room-temperature	311:326	arg1	transition					336:345	a room-temperature sol-gel transition	309:345	a room-temperature sol-gel transition	309:345	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	1	27	theme	recyclable	160:169	arg1	nanocomposites					190:203	a new class recyclable magnetic catalytic nanocomposites	148:203	a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	148:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	0	28	theme	α-Amylase	0:8	arg1	Ferria					10:15	α-Amylase@Ferria	0:15	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.	0:95	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	6	29	theme	ceramic	1132:1138	arg1	material					1043:1050	The material	1039:1050	The material	1039:1050	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	29	theme	ceramic	1132:1138	arg1	coating					1140:1146	a catalytic ceramic coating	1120:1146	a catalytic ceramic coating with at least 10 cycles of use	1120:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	30	used	used	1059:1062	arg2	catalyst					1108:1115	a magnetically separable reusable catalyst	1074:1115	a magnetically separable reusable catalyst	1074:1115	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	30	used	used	1059:1062	arg2	coating					1140:1146	a catalytic ceramic coating	1120:1146	a catalytic ceramic coating with at least 10 cycles of use	1120:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	6	30	used	used	1059:1062	arg2	material					1043:1050	The material	1039:1050	The material	1039:1050	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	1	31	theme	magnetic	171:178	arg1	nanocomposites					190:203	a new class recyclable magnetic catalytic nanocomposites	148:203	a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	148:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	5	32	from	days	944:947	arg1	solution					972:979	a buffer solution	963:979	a buffer solution	963:979	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	1	33	theme	catalytic	180:188	arg1	nanocomposites					190:203	a new class recyclable magnetic catalytic nanocomposites	148:203	a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	148:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	5	34	theme	buffer	965:970	arg1	solution					972:979	a buffer solution	963:979	a buffer solution	963:979	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	1	35	theme	present	101:107	arg1	devoted					118:124	devoted	118:124	devoted	118:124	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	1	35	theme	present	101:107	arg1	study					109:113	The present study	97:113	The present study	97:113	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	1	36	theme	nanocomposites	190:203	arg1	development					133:143	the development	129:143	the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis	129:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	6	37	with	catalyst	1108:1115	arg1	cycles					1165:1170	at least 10 cycles	1153:1170	at least 10 cycles of use	1153:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	5	38	theme	activity	926:933	arg1	%					917:917	only 9%	911:917	only 9% of its activity	911:933	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	5	38	theme	activity	926:933	arg1	activity					926:933	its activity	922:933	its activity	922:933	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	3	39	theme	hybrid	430:435	arg1	composites					454:463	hybrid organo-inorganic composites	430:463	hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %	430:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	2	40	theme	magnetic	409:416	arg1	matrix					418:423	a rigid magnetic matrix	401:423	a rigid magnetic matrix	401:423	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	0	41	theme	Magnetic	18:25	arg1	Nanocomposites					27:40	Magnetic Nanocomposites	18:40	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.	0:95	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	4	42	theme	composite	846:854	arg1	stability					829:837	higher thermal stability	814:837	higher thermal stability of the composite	814:854	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	1	43	theme	starch	209:214	arg1	hydrolysis					216:225	starch hydrolysis	209:225	starch hydrolysis	209:225	The present study is devoted to the development of a new class recyclable magnetic catalytic nanocomposites for starch hydrolysis.
29976057	0	44	theme	Enhanced	47:54	arg1	Stability					64:72	Enhanced Thermal Stability	47:72	Enhanced Thermal Stability	47:72	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	4	45	theme	higher	814:819	arg1	stability					829:837	higher thermal stability	814:837	higher thermal stability of the composite	814:854	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	3	46	theme	organo-inorganic	437:452	arg1	composites					454:463	hybrid organo-inorganic composites	430:463	hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %	430:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	4	47	theme	free	688:691	arg1	enzyme					693:698	free enzyme	688:698	free enzyme	688:698	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	48	dep	twice	760:764	arg1	higher					766:771	higher	766:771	higher	766:771	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	49	theme	ferria	566:571	arg1	composite					573:581	the amylase@ferria composite	554:581	the amylase@ferria composite	554:581	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	5	50	theme	storage	952:958	arg1	days					944:947	21 days	941:947	21 days of storage in a buffer solution	941:979	Entrapped amylase showed excellent stability and lost only 9% of its activity after 21 days of storage in a buffer solution, while free enzyme was totally inactivated after 17 days.
29976057	3	51	theme	wt	505:506	arg1	%					508:508	10 wt %	502:508	10 wt %	502:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	4	52	theme	stability	829:837	arg1	result					804:809	a result	802:809	a result of higher thermal stability of the composite	802:854	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	53	theme	@	740:740	arg1	ferria					741:746	amylase@ferria	733:746	amylase@ferria	733:746	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	54	theme	magnetite-derived	261:277	arg1	matrix					287:292	a magnetite-derived xerogel matrix	259:292	a magnetite-derived xerogel matrix	259:292	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	4	55	theme	starch	651:656	arg1	rate					669:672	the starch hydrolysis rate	647:672	the starch hydrolysis rate comparable to free enzyme	647:698	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	56	theme	transition	336:345	arg1	course					299:304	a course	297:304	a course of a room-temperature sol-gel transition	297:345	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	3	57	with	composites	454:463	arg1	fractions					482:490	enzyme mass fractions	470:490	enzyme mass fractions less than 10 wt %	470:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	0	58	dep	Ferria	10:15	arg1	Nanocomposites					27:40	Magnetic Nanocomposites	18:40	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.	0:95	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	2	59	theme	sol-gel	328:334	arg1	transition					336:345	a room-temperature sol-gel transition	309:345	a room-temperature sol-gel transition	309:345	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	3	60	theme	less	492:495	arg1	fractions					482:490	enzyme mass fractions	470:490	enzyme mass fractions less than 10 wt %	470:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	4	61	from	activity	606:613	arg1	level					622:626	the level	618:626	the level of 10 units/mg	618:641	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	2	62	theme	rigid	403:407	arg1	matrix					418:423	a rigid magnetic matrix	401:423	a rigid magnetic matrix	401:423	α-Amylase was entrapped within a magnetite-derived xerogel matrix in a course of a room-temperature sol-gel transition, leading to enzyme immobilization within the pores of a rigid magnetic matrix.
29976057	3	63	theme	mass	477:480	arg1	fractions					482:490	enzyme mass fractions	470:490	enzyme mass fractions less than 10 wt %	470:508	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	4	64	theme	hydrolysis	658:667	arg1	rate					669:672	the starch hydrolysis rate	647:672	the starch hydrolysis rate comparable to free enzyme	647:698	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	0	65	theme	Thermal	56:62	arg1	Stability					64:72	Enhanced Thermal Stability	47:72	Enhanced Thermal Stability	47:72	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	4	66	theme	ferria	741:746	arg1	twice					760:764	twice	760:764	twice	760:764	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	4	66	theme	ferria	741:746	arg1	activity					721:728	the activity	717:728	the activity of amylase@ferria	717:746	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
29976057	0	67	with	Nanocomposites	27:40	arg1	Stability					64:72	Enhanced Thermal Stability	47:72	Enhanced Thermal Stability	47:72	α-Amylase@Ferria: Magnetic Nanocomposites with Enhanced Thermal Stability for Starch Hydrolysis.
29976057	3	68	theme	enzyme	514:519	arg1	leaching					521:528	no enzyme leaching	511:528	no enzyme leaching	511:528	For hybrid organo-inorganic composites with enzyme mass fractions less than 10 wt %, no enzyme leaching was observed.
29976057	6	69	with	coating	1140:1146	arg1	cycles					1165:1170	at least 10 cycles	1153:1170	at least 10 cycles of use	1153:1177	The material can be used as either a magnetically separable reusable catalyst or a catalytic ceramic coating with at least 10 cycles of use.
29976057	4	70	theme	catalytic	596:604	arg1	activity					606:613	catalytic activity	596:613	catalytic activity on the level of 10 units/mg	596:641	At 80 °C, the amylase@ferria composite demonstrates catalytic activity on the level of 10 units/mg and the starch hydrolysis rate comparable to free enzyme, while at 90 °C, the activity of amylase@ferria is at least twice higher than that of free amylase as a result of higher thermal stability of the composite.
30268077	3	0	theme	proteins	605:612	arg1	function					587:594	the function	583:594	the function of these proteins	583:612	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	1	from	changes	1075:1081	arg1	structure					1090:1098	the structure	1086:1098	the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1086:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	3	2	theme	βIII-Gal	803:810	arg1	plants					840:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	3	theme	arabidopsis	1107:1117	arg1	polysaccharides					1129:1143	the arabidopsis cell wall polysaccharides	1103:1143	the arabidopsis cell wall polysaccharides	1103:1143	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	3	theme	arabidopsis	1107:1117	arg1	reduction					1155:1163	a reduction	1153:1163	a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1153:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	4	4	theme	morphology	895:904	arg1	alterations					876:886	visible alterations	868:886	visible alterations of the morphology of the transgenic plant	868:928	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	4	4	theme	morphology	895:904	arg1	all					931:933	all	931:933	all	931:933	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	7	5	from	specificity	1582:1592	arg1	walls					1828:1832	the cell walls	1819:1832	the cell walls	1819:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	2	6	theme	developmental	498:510	arg1	processes					512:520	different developmental processes	488:520	different developmental processes according to previous studies	488:550	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	4	7	from	decrease	948:955	arg1	growth					960:965	growth	960:965	growth at different stages of development	960:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	4	7	from	decrease	948:955	arg1	stages					980:985	different stages	970:985	different stages of development	970:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	5	8	theme	galactan	1172:1179	arg1	chains					1186:1191	the galactan side chains	1168:1191	the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1168:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	9	theme	cell	1119:1122	arg1	polysaccharides					1129:1143	the arabidopsis cell wall polysaccharides	1103:1143	the arabidopsis cell wall polysaccharides	1103:1143	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	9	theme	cell	1119:1122	arg1	reduction					1155:1163	a reduction	1153:1163	a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1153:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	2	10	theme	sequence	447:454	arg1	identity					456:463	amino acid sequence identity	436:463	amino acid sequence identity (81%)	436:469	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	2	10	theme	sequence	447:454	arg1	%					468:468	81%	466:468	81%	466:468	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	1	11	theme	different	301:309	arg1	proteins					311:318	the different proteins	297:318	the different proteins	297:318	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
30268077	2	12	theme	different	488:496	arg1	processes					512:520	different developmental processes	488:520	different developmental processes according to previous studies	488:550	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	5	13	theme	architecture	1326:1337	arg1	control					1311:1317	the control	1307:1317	the control of the architecture of the cell wall	1307:1354	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	14	theme	side	1181:1184	arg1	chains					1186:1191	the galactan side chains	1168:1191	the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1168:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	15	theme	wall	1124:1127	arg1	polysaccharides					1129:1143	the arabidopsis cell wall polysaccharides	1103:1143	the arabidopsis cell wall polysaccharides	1103:1143	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	15	theme	wall	1124:1127	arg1	reduction					1155:1163	a reduction	1153:1163	a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1153:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	2	16	theme	acid	442:445	arg1	identity					456:463	amino acid sequence identity	436:463	amino acid sequence identity (81%)	436:469	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	2	16	theme	acid	442:445	arg1	%					468:468	81%	466:468	81%	466:468	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	1	17	theme	proteins	311:318	arg1	role					289:292	the role	285:292	the role of the different proteins	285:318	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
30268077	6	18	attach	present	1430:1436	arg2	plants					1416:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	6	18	attach	present	1430:1436	arg1	morphology					1479:1488	their morphology	1473:1488	their morphology	1473:1488	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	6	18	attach	present	1430:1436	arg1	walls					1508:1512	their cell walls	1497:1512	their cell walls	1497:1512	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	5	19	theme	marked	1219:1224	arg1	increase					1226:1233	a marked increase	1217:1233	a marked increase	1217:1233	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	3	20	theme	::	819:820	arg1	plants					840:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	21	theme	polysaccharides	1129:1143	arg1	structure					1090:1098	the structure	1086:1098	the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1086:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	2	22	theme	amino	436:440	arg1	identity					456:463	amino acid sequence identity	436:463	amino acid sequence identity (81%)	436:469	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	2	22	theme	amino	436:440	arg1	%					468:468	81%	466:468	81%	466:468	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	3	23	theme	substrate	636:644	arg1	specificity					646:656	their substrate specificity	630:656	their substrate specificity	630:656	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	24	theme	cell	1346:1349	arg1	wall					1351:1354	the cell wall	1342:1354	the cell wall	1342:1354	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	6	25	theme	cell	1503:1506	arg1	walls					1508:1512	their cell walls	1497:1512	their cell walls	1497:1512	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	3	26	theme	βIV-Gal	821:827	arg1	plants					840:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	0	27	theme	cell	96:99	arg1	-galactan					113:121	cell wall β-(1,4)-galactan	96:121	cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan	96:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	7	28	theme	sites	1734:1738	arg1	points					1694:1699	isoelectric points	1682:1699	isoelectric points	1682:1699	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	28	theme	sites	1734:1738	arg1	number					1708:1713	the number	1704:1713	the number of N-glycosylation sites	1704:1738	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	2	29	theme	previous	535:542	arg1	studies					544:550	previous studies	535:550	previous studies	535:550	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	5	30	theme	FTIR	1003:1006	arg1	spectroscopy					1008:1019	FTIR spectroscopy	1003:1019	FTIR spectroscopy	1003:1019	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	0	31	theme	wall	101:104	arg1	-galactan					113:121	cell wall β-(1,4)-galactan	96:121	cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan	96:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	2	32	theme	family	386:391	arg1	βIII-Gal					394:401	βIII-Gal	394:401	βIII-Gal	394:401	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	2	32	theme	family	386:391	arg1	members					370:376	2 members	368:376	2 members of this family	368:391	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	2	32	theme	family	386:391	arg1	βIV-Gal					407:413	βIV-Gal	407:413	βIV-Gal	407:413	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	1	33	theme	multigene	256:264	arg1	families					266:273	multigene families	256:273	multigene families	256:273	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
30268077	3	34	theme	35S	816:818	arg1	::					819:820	the 35S::βIII-Gal and 35S::	794:820	::	819:820	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	7	35	theme	cell	1823:1826	arg1	walls					1828:1832	the cell walls	1819:1832	the cell walls	1819:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	6	36	theme	35S	1403:1405	arg1	plants					1416:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	3	37	from	thaliana	769:776	arg1	overproduced					741:752	overproduced	741:752	overproduced	741:752	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	7	38	theme	sequence	1536:1543	arg1	homology					1545:1552	the high sequence homology	1527:1552	the high sequence homology	1527:1552	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	3	39	theme	possible	666:673	arg1	alterations					675:685	the possible alterations	662:685	the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	662:845	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	7	40	theme	dissimilar	1646:1655	arg1	points					1694:1699	isoelectric points	1682:1699	isoelectric points	1682:1699	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	40	theme	dissimilar	1646:1655	arg1	characteristics					1657:1671	other dissimilar characteristics	1640:1671	other dissimilar characteristics	1640:1671	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	40	theme	dissimilar	1646:1655	arg1	number					1708:1713	the number	1704:1713	the number of N-glycosylation sites	1704:1738	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	0	41	theme	Cicer	18:22	arg1	Overexpression					0:13	Overexpression	0:13	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis	0:72	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	0	42	theme	β-	106:107	arg1	-galactan					113:121	cell wall β-(1,4)-galactan	96:121	cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan	96:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	3	43	theme	transgenic	829:838	arg1	plants					840:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants	794:845	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	44	from	role	1299:1302	arg1	control					1311:1317	the control	1307:1317	the control of the architecture of the cell wall	1307:1354	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	44	from	role	1299:1302	arg1	processes					1378:1386	the processes	1374:1386	the processes of growth	1374:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	4	45	theme	transgenic	913:922	arg1	plant					924:928	the transgenic plant	909:928	the transgenic plant	909:928	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	0	46	from	increase	141:148	arg1	homogalacturonan					153:168	homogalacturonan	153:168	homogalacturonan	153:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	3	47	from	overproduced	741:752	arg1	thaliana					769:776	Arabidopsis thaliana	757:776	Arabidopsis thaliana	757:776	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	7	48	theme	enzymatic	1769:1777	arg1	properties					1779:1788	their enzymatic properties	1763:1788	their enzymatic properties	1763:1788	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	4	49	theme	plant	924:928	arg1	morphology					895:904	the morphology	891:904	the morphology of the transgenic plant	891:928	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	0	50	theme	1,4	109:111	arg1	-galactan					113:121	cell wall β-(1,4)-galactan	96:121	cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan	96:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	3	51	theme	cell	701:704	arg1	polysaccharides					711:725	the cell wall polysaccharides	697:725	the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana	697:776	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	3	52	theme	wall	706:709	arg1	polysaccharides					711:725	the cell wall polysaccharides	697:725	the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana	697:776	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	5	53	theme	growth	1391:1396	arg1	processes					1378:1386	the processes	1374:1386	the processes of growth	1374:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	7	54	theme	isoelectric	1682:1692	arg1	points					1694:1699	isoelectric points	1682:1699	isoelectric points	1682:1699	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	55	theme	N-glycosylation	1718:1732	arg1	sites					1734:1738	N-glycosylation sites	1718:1738	N-glycosylation sites	1718:1738	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	56	theme	different	1572:1580	arg1	specificity					1582:1592	different specificity	1572:1592	different specificity of substrate for these proteins	1572:1624	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	57	gly	N-glycosylation	1718:1732	arg2	sites					1734:1738	N-glycosylation sites	1718:1738	N-glycosylation sites	1718:1738	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	2	58	theme	identity	456:463	arg1	degree					426:431	high degree	421:431	high degree of amino acid sequence identity (81%)	421:469	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	7	59	theme	high	1531:1534	arg1	homology					1545:1552	the high sequence homology	1527:1552	the high sequence homology	1527:1552	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	0	60	theme	-galactan	113:121	arg1	reduction					83:91	a reduction	81:91	a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan	81:168	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	5	61	theme	wall	1351:1354	arg1	architecture					1326:1337	the architecture	1322:1337	the architecture of the cell wall	1322:1354	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	62	theme	chains	1186:1191	arg1	polysaccharides					1129:1143	the arabidopsis cell wall polysaccharides	1103:1143	the arabidopsis cell wall polysaccharides	1103:1143	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	5	62	theme	chains	1186:1191	arg1	reduction					1155:1163	a reduction	1153:1163	a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth	1153:1396	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	4	63	theme	development	990:1000	arg1	stages					980:985	different stages	970:985	different stages of development	970:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	5	64	from	increase	1226:1233	arg1	homogalacturonan					1238:1253	homogalacturonan	1238:1253	homogalacturonan	1238:1253	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	4	65	from	stages	980:985	arg1	growth					960:965	growth	960:965	growth at different stages of development	960:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	4	65	from	stages	980:985	arg1	decrease					948:955	a decrease	946:955	a decrease in growth at different stages of development	946:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	4	66	theme	different	970:978	arg1	stages					980:985	different stages	970:985	different stages of development	970:1000	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	6	67	theme	phenotypic	1442:1451	arg1	changes					1453:1459	any phenotypic changes	1438:1459	any phenotypic changes	1438:1459	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	0	68	dep	Cicer	18:22	arg1	arietinum					24:32	Cicer arietinum	18:32	Cicer arietinum βIII-Gal but not βIV-Gal	18:57	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	3	69	theme	Arabidopsis	757:767	arg1	thaliana					769:776	Arabidopsis thaliana	757:776	Arabidopsis thaliana	757:776	Our objective is to deepen in the function of these proteins by establishing their substrate specificity and the possible alterations caused in the cell wall polysaccharides when they are overproduced in Arabidopsis thaliana by constructing the 35S::βIII-Gal and 35S::βIV-Gal transgenic plants.
30268077	6	70	theme	::	1406:1407	arg1	plants					1416:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	6	71	theme	βIV-Gal	1408:1414	arg1	plants					1416:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants	1399:1421	The 35S::βIV-Gal plants do not present any phenotypic changes, neither in their morphology nor in their cell walls.
30268077	7	72	from	action	1809:1814	arg1	walls					1828:1832	the cell walls	1819:1832	the cell walls	1819:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	73	theme	other	1640:1644	arg1	points					1694:1699	isoelectric points	1682:1699	isoelectric points	1682:1699	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	73	theme	other	1640:1644	arg1	characteristics					1657:1671	other dissimilar characteristics	1640:1671	other dissimilar characteristics	1640:1671	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	73	theme	other	1640:1644	arg1	number					1708:1713	the number	1704:1713	the number of N-glycosylation sites	1704:1738	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	74	from	maybe	1627:1631	arg1	walls					1828:1832	the cell walls	1819:1832	the cell walls	1819:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	75	theme	substrate	1597:1605	arg1	specificity					1582:1592	different specificity	1572:1592	different specificity of substrate for these proteins	1572:1624	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	75	theme	substrate	1597:1605	arg1	maybe					1627:1631	maybe	1627:1631	maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties	1627:1788	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	7	75	theme	substrate	1597:1605	arg1	action					1809:1814	their distinct action	1794:1814	their distinct action in the cell walls	1794:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	1	76	theme	several	197:203	arg1	species					211:217	several plant species	197:217	several plant species	197:217	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
30268077	2	77	theme	high	421:424	arg1	degree					426:431	high degree	421:431	high degree of amino acid sequence identity (81%)	421:469	Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%), but involved in different developmental processes according to previous studies.
30268077	5	78	theme	immunological	1025:1037	arg1	studies					1039:1045	immunological studies	1025:1045	immunological studies	1025:1045	FTIR spectroscopy and immunological studies showed that βIII-Gal causes changes in the structure of the arabidopsis cell wall polysaccharides, mainly a reduction of the galactan side chains which is compensated by a marked increase in homogalacturonan, which allows us to attribute to galactan a role in the control of the architecture of the cell wall, and therefore in the processes of growth.
30268077	1	79	dep	Cicer	174:178	arg1	arietinum					180:188	Cicer arietinum	174:188	Cicer arietinum	174:188	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
30268077	0	80	from	Overexpression	0:13	arg1	arabidopsis					62:72	arabidopsis	62:72	arabidopsis	62:72	Overexpression of Cicer arietinum βIII-Gal but not βIV-Gal in arabidopsis causes a reduction of cell wall β-(1,4)-galactan compensated by an increase in homogalacturonan.
30268077	4	81	theme	visible	868:874	arg1	alterations					876:886	visible alterations	868:886	visible alterations of the morphology of the transgenic plant	868:928	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	4	81	theme	visible	868:874	arg1	all					931:933	all	931:933	all	931:933	βIII-Gal does cause visible alterations of the morphology of the transgenic plant, all related to a decrease in growth at different stages of development.
30268077	7	82	theme	distinct	1800:1807	arg1	action					1809:1814	their distinct action	1794:1814	their distinct action in the cell walls	1794:1832	In spite of the high sequence homology, our results show different specificity of substrate for these proteins, maybe due to other dissimilar characteristics, such as isoelectric points or the number of N-glycosylation sites, which could determine their enzymatic properties and their distinct action in the cell walls.
30268077	1	83	theme	plant	205:209	arg1	species					211:217	several plant species	197:217	several plant species	197:217	In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated.
31532880	4	0	theme	aqueous	722:728	arg1	method					745:750	an aqueous thermochemical method	719:750	an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials	719:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	4	0	theme	aqueous	722:728	arg1	processing					707:716	hydrothermal processing	694:716	hydrothermal processing	694:716	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	1	1	from	applications	190:201	arg1	measurements					259:270	in situ strain measurements	244:270	in situ strain measurements	244:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	1	from	applications	190:201	arg1	healthcare					206:215	healthcare	206:215	healthcare	206:215	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	1	from	applications	190:201	arg1	motion					233:238	tracking human motion	218:238	tracking human motion	218:238	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	2	theme	flexible	164:171	arg1	electronics					173:183	flexible electronics	164:183	flexible electronics	164:183	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	3	theme	strain	252:257	arg1	measurements					259:270	in situ strain measurements	244:270	in situ strain measurements	244:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	4	with	electronics	173:183	arg1	applications					190:201	applications	190:201	applications in healthcare, tracking human motion, or in situ strain measurements	190:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	5	theme	electronics	173:183	arg1	manufacturing					136:148	the manufacturing	132:148	the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements	132:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	6	with	sensors	153:159	arg1	applications					190:201	applications	190:201	applications in healthcare, tracking human motion, or in situ strain measurements	190:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	4	7	theme	composite	863:871	arg1	biomaterials					873:884	composite biomaterials	863:884	composite biomaterials	863:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	3	8	theme	conductive	546:555	arg1	biomaterials					627:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	5	9	theme	conductive	1007:1016	arg1	biomaterials					1028:1039	these conductive composite biomaterials	1001:1039	these conductive composite biomaterials	1001:1039	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	2	10	dep	ex	477:478	arg1	vivo					480:483	vivo	480:483	vivo	480:483	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	11	from	interface	353:361	arg1	mismatch					312:319	the mismatch	308:319	the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications	308:496	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	12	theme	typical	371:377	arg1	sensors					399:405	typical rigid semiconductor sensors	371:405	typical rigid semiconductor sensors	371:405	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	3	13	dep	conductive	546:555	arg1	stretchable					565:575	stretchable	565:575	stretchable	565:575	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	1	14	theme	tracking	218:225	arg1	motion					233:238	tracking human motion	218:238	tracking human motion	218:238	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	1	15	theme	great	75:79	arg1	interest					81:88	great interest	75:88	great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements	75:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	5	16	theme	molecular	929:937	arg1	modeling					948:955	full-atomistic molecular dynamics modeling	914:955	full-atomistic molecular dynamics modeling	914:955	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	2	17	theme	in	465:466	arg1	applications					485:496	in vivo and ex vivo applications	465:496	in vivo and ex vivo applications	465:496	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	18	theme	soft	411:414	arg1	surfaces					441:448	biological surfaces	430:448	biological surfaces	430:448	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	0	19	theme	Silk-Based	11:20	arg1	Composites					22:31	Conductive Silk-Based Composites	0:31	Conductive Silk-Based Composites	0:31	Conductive Silk-Based Composites Using Biobased Carbon Materials.
31532880	2	20	dep	in	465:466	arg1	vivo					468:471	vivo	468:471	vivo	468:471	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	21	theme	mechanical	324:333	arg1	properties					335:344	mechanical properties	324:344	mechanical properties	324:344	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	4	22	theme	hydrothermal	694:705	arg1	method					745:750	an aqueous thermochemical method	719:750	an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials	719:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	4	22	theme	hydrothermal	694:705	arg1	processing					707:716	hydrothermal processing	694:716	hydrothermal processing	694:716	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	0	23	theme	Conductive	0:9	arg1	Composites					22:31	Conductive Silk-Based Composites	0:31	Conductive Silk-Based Composites	0:31	Conductive Silk-Based Composites Using Biobased Carbon Materials.
31532880	5	24	theme	composite	1018:1026	arg1	biomaterials					1028:1039	these conductive composite biomaterials	1001:1039	these conductive composite biomaterials	1001:1039	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	5	25	theme	dynamics	939:946	arg1	modeling					948:955	full-atomistic molecular dynamics modeling	914:955	full-atomistic molecular dynamics modeling	914:955	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	1	26	theme	conductive	104:113	arg1	biomaterials					115:126	conductive biomaterials	104:126	conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements	104:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	3	27	theme	carbons	525:531	arg1	use					509:511	the use	505:511	the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	505:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	1	28	dep	in	244:245	arg1	situ					247:250	situ	247:250	situ	247:250	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	5	29	from	applications	1098:1109	arg1	fields					1114:1119	fields	1114:1119	fields like biomedicine, energy, and electronics	1114:1161	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	2	30	theme	biological	430:439	arg1	surfaces					441:448	biological surfaces	430:448	biological surfaces	430:448	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	0	31	theme	Carbon	48:53	arg1	Materials					55:63	Biobased Carbon Materials	39:63	Biobased Carbon Materials	39:63	Conductive Silk-Based Composites Using Biobased Carbon Materials.
31532880	5	32	theme	promising	1088:1096	arg1	applications					1098:1109	promising applications	1088:1109	promising applications in fields like biomedicine, energy, and electronics	1088:1161	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	5	33	theme	Experimental	887:898	arg1	synthesis					900:908	Experimental synthesis	887:908	Experimental synthesis	887:908	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	1	34	theme	human	227:231	arg1	motion					233:238	tracking human motion	218:238	tracking human motion	218:238	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	0	35	theme	Biobased	39:46	arg1	Materials					55:63	Biobased Carbon Materials	39:63	Biobased Carbon Materials	39:63	Conductive Silk-Based Composites Using Biobased Carbon Materials.
31532880	2	36	theme	ex	477:478	arg1	applications					485:496	in vivo and ex vivo applications	465:496	in vivo and ex vivo applications	465:496	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	3	37	theme	biocompatible	592:604	arg1	biomaterials					627:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	4	38	theme	Biobased	657:664	arg1	carbons					666:672	Biobased carbons	657:672	Biobased carbons	657:672	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	3	39	theme	flexible	578:585	arg1	biomaterials					627:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	4	40	from	filler	853:858	arg1	biomaterials					873:884	composite biomaterials	863:884	composite biomaterials	863:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	4	41	theme	thermochemical	730:743	arg1	method					745:750	an aqueous thermochemical method	719:750	an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials	719:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	4	41	theme	thermochemical	730:743	arg1	processing					707:716	hydrothermal processing	694:716	hydrothermal processing	694:716	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	3	42	theme	composite	617:625	arg1	biomaterials					627:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	1	43	theme	sensors	153:159	arg1	manufacturing					136:148	the manufacturing	132:148	the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements	132:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	4	44	theme	conductive	842:851	arg1	filler					853:858	conductive filler	842:858	conductive filler in composite biomaterials	842:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	4	45	theme	carbonaceous	781:792	arg1	material					794:801	a carbonaceous material	779:801	a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials	779:884	Biobased carbons are synthesized via hydrothermal processing, an aqueous thermochemical method that converts biomass into a carbonaceous material that can be applied upon activation as conductive filler in composite biomaterials.
31532880	5	46	theme	renewable	1061:1069	arg1	sources					1071:1077	renewable sources	1061:1077	renewable sources	1061:1077	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	3	47	theme	biobased	516:523	arg1	carbons					525:531	biobased carbons	516:531	biobased carbons	516:531	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	3	48	theme	silk-based	606:615	arg1	biomaterials					627:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials	546:638	Here, the use of biobased carbons to fabricate conductive, highly stretchable, flexible, and biocompatible silk-based composite biomaterials is demonstrated.
31532880	2	49	theme	semiconductor	385:397	arg1	sensors					399:405	typical rigid semiconductor sensors	371:405	typical rigid semiconductor sensors	371:405	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	5	50	theme	full-atomistic	914:927	arg1	modeling					948:955	full-atomistic molecular dynamics modeling	914:955	full-atomistic molecular dynamics modeling	914:955	Experimental synthesis and full-atomistic molecular dynamics modeling are combined to synthesize and characterize these conductive composite biomaterials, made entirely from renewable sources and with promising applications in fields like biomedicine, energy, and electronics.
31532880	1	51	theme	in	244:245	arg1	measurements					259:270	in situ strain measurements	244:270	in situ strain measurements	244:270	There is great interest in developing conductive biomaterials for the manufacturing of sensors or flexible electronics with applications in healthcare, tracking human motion, or in situ strain measurements.
31532880	2	52	dep	soft	411:414	arg1	uneven					423:428	uneven	423:428	uneven	423:428	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	53	theme	rigid	379:383	arg1	sensors					399:405	typical rigid semiconductor sensors	371:405	typical rigid semiconductor sensors	371:405	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
31532880	2	54	from	mismatch	312:319	arg1	properties					335:344	mechanical properties	324:344	mechanical properties	324:344	These biomaterials aim to overcome the mismatch in mechanical properties at the interface between typical rigid semiconductor sensors and soft, often uneven biological surfaces or tissues for in vivo and ex vivo applications.
29580407	3	0	dep	44	480:481	arg1	to					477:478	to	477:478	to	477:478	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	6	1	from	reusability	1061:1071	arg1	cycles					1124:1129	at least five consecutive adsorption/desorption cycles	1076:1129	at least five consecutive adsorption/desorption cycles	1076:1129	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	6	2	theme	Reuse	930:934	arg1	experiments					936:946	Reuse experiments	930:946	Reuse experiments	930:946	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	0	3	theme	contaminants	111:122	arg1	removal					85:91	removal	85:91	removal of pharmaceutical contaminants from water	85:133	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	2	4	with	interaction	327:337	arg1	matrix					358:363	polymeric matrix	348:363	polymeric matrix	348:363	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	2	4	with	interaction	327:337	arg1	dispersibility					374:387	good dispersibility	369:387	good dispersibility	369:387	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	6	5	theme	remarkable	1050:1059	arg1	reusability					1061:1071	a remarkable reusability	1048:1071	a remarkable reusability in at least five consecutive adsorption/desorption cycles	1048:1129	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	5	6	theme	pseudo-second	830:842	arg1	model					850:854	the pseudo-second order model	826:854	the pseudo-second order model	826:854	For all pharmaceuticals, the adsorption kinetics was found to follow the pseudo-second order model, while the adsorption mechanism was explained by the Freundlich isotherm.
29580407	0	7	theme	pharmaceutical	96:109	arg1	contaminants					111:122	pharmaceutical contaminants	96:122	pharmaceutical contaminants from water	96:133	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	3	8	theme	noticeable	445:454	arg1	enhancement					456:466	a noticeable enhancement	443:466	a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	443:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	6	9	theme	WCG-containing	967:980	arg1	composite					982:990	the WCG-containing composite	963:990	the WCG-containing composite	963:990	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	1	10	theme	explored	173:180	arg1	source					182:187	a poorly explored source	164:187	a poorly explored source of biocompounds	164:203	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	1	10	theme	explored	173:180	arg1	coffee-grounds					142:155	Waste coffee-grounds	136:155	Waste coffee-grounds (WCG)	136:161	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	0	11	theme	coffee-grounds	15:28	arg1	composite					30:38	Chitosan/waste coffee-grounds composite	0:38	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.	0:134	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	2	12	theme	good	322:325	arg1	interaction					327:337	a good interaction	320:337	a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%	320:401	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	0	13	theme	Chitosan/waste	0:13	arg1	composite					30:38	Chitosan/waste coffee-grounds composite	0:38	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.	0:134	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	1	14	theme	vinyl	248:252	arg1	poly					243:246	poly	243:246	poly(vinyl alcohol) (PVA)	243:267	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	1	14	theme	vinyl	248:252	arg1	alcohol					254:260	vinyl alcohol	248:260	vinyl alcohol	248:260	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	5	15	theme	Freundlich	909:918	arg1	isotherm					920:927	the Freundlich isotherm	905:927	the Freundlich isotherm	905:927	For all pharmaceuticals, the adsorption kinetics was found to follow the pseudo-second order model, while the adsorption mechanism was explained by the Freundlich isotherm.
29580407	4	16	theme	removal	657:663	arg1	efficiency					665:674	The highest removal efficiency	645:674	The highest removal efficiency	645:674	The highest removal efficiency was registered at pH 6 and the removal followed the order ASA > CAF > ACE > MET.
29580407	5	17	theme	adsorption	786:795	arg1	kinetics					797:804	the adsorption kinetics	782:804	the adsorption kinetics	782:804	For all pharmaceuticals, the adsorption kinetics was found to follow the pseudo-second order model, while the adsorption mechanism was explained by the Freundlich isotherm.
29580407	1	18	theme	biocompounds	192:203	arg1	source					182:187	a poorly explored source	164:187	a poorly explored source of biocompounds	164:203	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	1	18	theme	biocompounds	192:203	arg1	coffee-grounds					142:155	Waste coffee-grounds	136:155	Waste coffee-grounds (WCG)	136:161	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	2	19	theme	good	369:372	arg1	dispersibility					374:387	good dispersibility	369:387	good dispersibility	369:387	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	MET					534:536	MET	534:536	MET	534:536	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	acetaminophen					568:580	acetaminophen	568:580	acetaminophen (ACE)	568:586	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	pharmaceuticals					506:520	pharmaceuticals	506:520	pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	506:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	caffeine					593:600	caffeine	593:600	caffeine	593:600	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	metamizol					523:531	metamizol	523:531	metamizol (MET)	523:537	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	CAF					603:605	CAF	603:605	CAF	603:605	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	acid					556:559	acetylsalicylic acid	540:559	acetylsalicylic acid (ASA)	540:565	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	ACE					583:585	ACE	583:585	ACE	583:585	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	20	dep	pharmaceuticals	506:520	arg1	ASA					562:564	ASA	562:564	ASA	562:564	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	0	21	from	removal	85:91	arg1	water					129:133	water	129:133	water	129:133	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	4	22	theme	highest	649:655	arg1	efficiency					665:674	The highest removal efficiency	645:674	The highest removal efficiency	645:674	The highest removal efficiency was registered at pH 6 and the removal followed the order ASA > CAF > ACE > MET.
29580407	2	23	theme	polymeric	348:356	arg1	matrix					358:363	polymeric matrix	348:363	polymeric matrix	348:363	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	3	24	theme	adsorption	492:501	arg1	enhancement					456:466	a noticeable enhancement	443:466	a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	443:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	6	25	contain	has	992:994	arg1	composite					982:990	the WCG-containing composite	963:990	the WCG-containing composite	963:990	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	6	25	contain	has	992:994	arg2	cost-effectiveness					1010:1027	an attractive cost-effectiveness	996:1027	an attractive cost-effectiveness	996:1027	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	6	26	theme	consecutive	1090:1100	arg1	cycles					1124:1129	at least five consecutive adsorption/desorption cycles	1076:1129	at least five consecutive adsorption/desorption cycles	1076:1129	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	2	27	theme	10 wt-	395:400	arg1	%					401:401	10 wt-%	395:401	10 wt-%	395:401	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	0	28	from	water	129:133	arg1	removal					85:91	removal	85:91	removal of pharmaceutical contaminants from water	85:133	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	0	28	from	water	129:133	arg1	contaminants					111:122	pharmaceutical contaminants	96:122	pharmaceutical contaminants from water	96:133	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	2	29	dep	matrix	358:363	arg1	the					344:346	the	344:346	the	344:346	Overall, WCG showed a good interaction with the polymeric matrix and good dispersibility up to 10 wt-%.
29580407	1	30	theme	Waste	136:140	arg1	coffee-grounds					142:155	Waste coffee-grounds	136:155	Waste coffee-grounds (WCG)	136:161	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	1	30	theme	Waste	136:140	arg1	source					182:187	a poorly explored source	164:187	a poorly explored source of biocompounds	164:203	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	1	30	theme	Waste	136:140	arg1	WCG					158:160	WCG	158:160	WCG	158:160	Waste coffee-grounds (WCG), a poorly explored source of biocompounds, were combined with chitosan (Cs) and poly(vinyl alcohol) (PVA) in order to obtain composites.
29580407	6	31	theme	adsorption/desorption	1102:1122	arg1	cycles					1124:1129	at least five consecutive adsorption/desorption cycles	1076:1129	at least five consecutive adsorption/desorption cycles	1076:1129	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	3	32	theme	5 wt-	407:411	arg1	WCG					414:416	5 wt-% WCG	407:416	5 wt-% WCG	407:416	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	6	33	theme	attractive	999:1008	arg1	cost-effectiveness					1010:1027	an attractive cost-effectiveness	996:1027	an attractive cost-effectiveness	996:1027	Reuse experiments indicated that the WCG-containing composite has an attractive cost-effectiveness since it presented a remarkable reusability in at least five consecutive adsorption/desorption cycles.
29580407	4	34	theme	order	728:732	arg1	ASA > CAF > ACE > MET					734:754	the order ASA > CAF > ACE > MET	724:754	the order ASA > CAF > ACE > MET	724:754	The highest removal efficiency was registered at pH 6 and the removal followed the order ASA > CAF > ACE > MET.
29580407	3	35	theme	acetylsalicylic	540:554	arg1	acid					556:559	acetylsalicylic acid	540:559	acetylsalicylic acid (ASA)	540:565	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	35	theme	acetylsalicylic	540:554	arg1	pharmaceuticals					506:520	pharmaceuticals	506:520	pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	506:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	35	theme	acetylsalicylic	540:554	arg1	ASA					562:564	ASA	562:564	ASA	562:564	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	0	36	dep	composite	30:38	arg1	efficient					44:52	efficient	44:52	efficient	44:52	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	0	36	dep	composite	30:38	arg1	adsorbent					71:79	adsorbent	71:79	adsorbent	71:79	Chitosan/waste coffee-grounds composite: An efficient and eco-friendly adsorbent for removal of pharmaceutical contaminants from water.
29580407	3	37	theme	%	412:412	arg1	WCG					414:416	5 wt-% WCG	407:416	5 wt-% WCG	407:416	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	5	38	theme	adsorption	867:876	arg1	mechanism					878:886	the adsorption mechanism	863:886	the adsorption mechanism	863:886	For all pharmaceuticals, the adsorption kinetics was found to follow the pseudo-second order model, while the adsorption mechanism was explained by the Freundlich isotherm.
29580407	5	39	theme	order	844:848	arg1	model					850:854	the pseudo-second order model	826:854	the pseudo-second order model	826:854	For all pharmaceuticals, the adsorption kinetics was found to follow the pseudo-second order model, while the adsorption mechanism was explained by the Freundlich isotherm.
29580407	3	40	from	%	482:482	arg1	enhancement					456:466	a noticeable enhancement	443:466	a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	443:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	41	theme	pharmaceuticals	506:520	arg1	adsorption					492:501	the adsorption	488:501	the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF))	488:607	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
29580407	3	42	theme	pristine	628:635	arg1	sample					637:642	the pristine sample	624:642	the pristine sample	624:642	At 5 wt-% WCG, the composite exhibited a noticeable enhancement (from 10 to 44%) of the adsorption of pharmaceuticals (metamizol (MET), acetylsalicylic acid (ASA), acetaminophen (ACE), and caffeine (CAF)) as compared to the pristine sample.
31733257	2	0	dep	0.73	458:461	arg1	to					455:456	to	455:456	to	455:456	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	1	1	theme	fruiting	172:179	arg1	bodies					181:186	fruiting bodies	172:186	fruiting bodies of Russula virescens (RVP)	172:213	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	2	2	with	derivatives	329:339	arg1	degrees					408:414	different degrees	398:414	different degrees of substitution (DS) ranging from 0.34 to 0.73	398:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	8	3	theme	anticoagulant	1076:1088	arg1	activities					1120:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	0	4	from	modification	9:20	arg1	bodies					105:110	the fruiting bodies	92:110	the fruiting bodies of Russula virescens	92:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	10	5	theme	antibacterial	1484:1496	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	3	6	theme	RVP	509:511	arg1	features					497:504	Several structural features	478:504	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation	478:619	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	9	7	dep	ABTS	1220:1223	arg1	activities					1257:1266	scavenging activities	1246:1266	scavenging activities	1246:1266	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	9	8	theme	scavenging	1246:1255	arg1	activities					1257:1266	scavenging activities	1246:1266	scavenging activities	1246:1266	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	9	9	theme	antibacterial	1327:1339	arg1	activity					1341:1348	antibacterial activity	1327:1348	antibacterial activity	1327:1348	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	1	10	from	bodies	181:186	arg1	polysaccharide					152:165	The water-soluble polysaccharide	134:165	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP)	134:213	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	2	11	theme	sulfated	316:323	arg1	derivatives					329:339	Five sulfated RVP derivatives	311:339	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73	311:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	11	theme	sulfated	316:323	arg1	SRVP1-25					383:390	SRVP1-25	383:390	SRVP1-25	383:390	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	11	theme	sulfated	316:323	arg1	SRVP1-20					370:377	SRVP1-20	370:377	SRVP1-20	370:377	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	1	12	theme	different	280:288	arg1	conditions					299:308	different reaction conditions	280:308	different reaction conditions	280:308	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	0	13	theme	fruiting	96:103	arg1	bodies					105:110	the fruiting bodies	92:110	the fruiting bodies of Russula virescens	92:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	14	from	bioactivities	55:67	arg1	bodies					105:110	the fruiting bodies	92:110	the fruiting bodies of Russula virescens	92:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	10	15	theme	anticoagulant	1469:1481	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	8	16	theme	In	993:994	arg1	results					1016:1022	In vitro activity test results	993:1022	In vitro activity test results	993:1022	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	1	17	theme	reaction	290:297	arg1	conditions					299:308	different reaction conditions	280:308	different reaction conditions	280:308	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	0	18	theme	Russula	115:121	arg1	virescens					123:131	Russula virescens	115:131	Russula virescens	115:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	3	19	theme	structural	486:495	arg1	features					497:504	Several structural features	478:504	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation	478:619	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	10	20	theme	industrial	1524:1533	arg1	use					1550:1552	industrial and biomedical use	1524:1552	industrial and biomedical use	1524:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	3	21	theme	Several	478:484	arg1	features					497:504	Several structural features	478:504	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation	478:619	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	4	22	theme	sulfate	679:685	arg1	groups					687:692	the sulfate groups	675:692	the sulfate groups	675:692	The spectra results indicated that the sulfate groups were successfully introduced on RVP.
31733257	9	23	theme	anti-tumor	1354:1363	arg1	activity					1365:1372	anti-tumor activity	1354:1372	anti-tumor activity	1354:1372	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	10	24	theme	biomedical	1539:1548	arg1	use					1550:1552	industrial and biomedical use	1524:1552	industrial and biomedical use	1524:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	8	25	theme	activity	1002:1009	arg1	results					1016:1022	In vitro activity test results	993:1022	In vitro activity test results	993:1022	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	3	26	theme	chemical	533:540	arg1	structure					542:550	chemical structure	533:550	chemical structure	533:550	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	1	27	theme	Russula	191:197	arg1	virescens					199:207	Russula virescens	191:207	Russula virescens (RVP)	191:213	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	1	27	theme	Russula	191:197	arg1	RVP					210:212	RVP	210:212	RVP	210:212	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	6	28	theme	molar	885:889	arg1	ratios					891:896	different molar ratios	875:896	different molar ratios	875:896	Both RVP and SRVPs were mainly composed of mannose, glucose and galactose, with different molar ratios.
31733257	10	29	theme	agents	1513:1518	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	10	29	theme	agents	1513:1518	arg1	one					1439:1441	one	1439:1441	one	1439:1441	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	10	29	theme	agents	1513:1518	arg1	SRVPs					1411:1415	the SRVPs	1407:1415	the SRVPs	1407:1415	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	7	30	theme	random	954:959	arg1	coil					961:964	a random coil	952:964	a random coil	952:964	The triple-helical conformation was broken down into a random coil when the DS exceeded 0.34.
31733257	1	31	theme	virescens	199:207	arg1	bodies					181:186	fruiting bodies	172:186	fruiting bodies of Russula virescens (RVP)	172:213	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	9	32	theme	best	1322:1325	arg1	activity					1341:1348	antibacterial activity	1327:1348	antibacterial activity	1327:1348	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	8	33	theme	antioxidant	1063:1073	arg1	activities					1120:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	9	34	theme	hydroxyl	1229:1236	arg1	radical					1238:1244	hydroxyl radical	1229:1244	hydroxyl radical	1229:1244	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	3	35	theme	molecular	581:589	arg1	weight					591:596	molecular weight	581:596	molecular weight	581:596	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	8	36	dep	In	993:994	arg1	vitro					996:1000	vitro	996:1000	vitro	996:1000	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	10	37	theme	potential	1446:1454	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	8	38	theme	test	1011:1014	arg1	results					1016:1022	In vitro activity test results	993:1022	In vitro activity test results	993:1022	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	3	39	theme	monosaccharide	553:566	arg1	composition					568:578	monosaccharide composition	553:578	monosaccharide composition	553:578	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	10	40	theme	anti-tumor	1502:1511	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	0	41	theme	virescens	123:131	arg1	bodies					105:110	the fruiting bodies	92:110	the fruiting bodies of Russula virescens	92:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	6	42	theme	different	875:883	arg1	ratios					891:896	different molar ratios	875:896	different molar ratios	875:896	Both RVP and SRVPs were mainly composed of mannose, glucose and galactose, with different molar ratios.
31733257	2	43	theme	RVP	325:327	arg1	derivatives					329:339	Five sulfated RVP derivatives	311:339	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73	311:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	43	theme	RVP	325:327	arg1	SRVP1-25					383:390	SRVP1-25	383:390	SRVP1-25	383:390	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	43	theme	RVP	325:327	arg1	SRVP1-20					370:377	SRVP1-20	370:377	SRVP1-20	370:377	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	4	44	theme	spectra	644:650	arg1	results					652:658	The spectra results	640:658	The spectra results	640:658	The spectra results indicated that the sulfate groups were successfully introduced on RVP.
31733257	5	45	theme	molecular	735:743	arg1	weights					745:751	The molecular weights	731:751	The molecular weights of SRVP	731:759	The molecular weights of SRVP decreased with the increasing DS.
31733257	3	46	theme	chain	602:606	arg1	conformation					608:619	chain conformation	602:619	chain conformation	602:619	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	2	47	theme	substitution	419:430	arg1	degrees					408:414	different degrees	398:414	different degrees of substitution (DS) ranging from 0.34 to 0.73	398:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	8	48	theme	antibacterial	1091:1103	arg1	activities					1120:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	5	49	theme	SRVP	756:759	arg1	weights					745:751	The molecular weights	731:751	The molecular weights of SRVP	731:759	The molecular weights of SRVP decreased with the increasing DS.
31733257	9	50	theme	strongest	1210:1218	arg1	ABTS					1220:1223	the strongest ABTS	1206:1223	the strongest ABTS	1206:1223	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	10	51	theme	antioxidant	1456:1466	arg1	agents					1513:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents	1446:1518	potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use	1446:1552	These results demonstrated that the SRVPs could be developed as one of potential antioxidant, anticoagulant, antibacterial and anti-tumor agents for industrial and biomedical use.
31733257	9	52	theme	anticoagulant	1272:1284	arg1	activity					1286:1293	anticoagulant activity	1272:1293	anticoagulant activity	1272:1293	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	2	53	dep	derivatives	329:339	arg1	SRVP1-20					370:377	SRVP1-20	370:377	SRVP1-20	370:377	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	53	dep	derivatives	329:339	arg1	derivatives					329:339	Five sulfated RVP derivatives	311:339	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73	311:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	2	53	dep	derivatives	329:339	arg1	SRVP1-25					383:390	SRVP1-25	383:390	SRVP1-25	383:390	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	0	54	theme	potential	45:53	arg1	bioactivities					55:67	potential bioactivities	45:67	potential bioactivities	45:67	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	9	55	dep	activity	1341:1348	arg1	the					1318:1320	the	1318:1320	the	1318:1320	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	8	56	theme	anti-tumor	1109:1118	arg1	activities					1120:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	better antioxidant, anticoagulant, antibacterial and anti-tumor activities	1056:1129	In vitro activity test results indicated that the SRVPs showed better antioxidant, anticoagulant, antibacterial and anti-tumor activities compared with RVP.
31733257	0	57	from	bodies	105:110	arg1	bioactivities					55:67	potential bioactivities	45:67	potential bioactivities	45:67	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	57	from	bodies	105:110	arg1	modification					9:20	modification	9:20	modification	9:20	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	57	from	bodies	105:110	arg1	characterization					23:38	characterization	23:38	characterization	23:38	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	57	from	bodies	105:110	arg1	polysaccharide					72:85	polysaccharide	72:85	polysaccharide from the fruiting bodies of Russula virescens	72:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	1	58	theme	water-soluble	138:150	arg1	polysaccharide					152:165	The water-soluble polysaccharide	134:165	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP)	134:213	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	7	59	theme	triple-helical	903:916	arg1	conformation					918:929	The triple-helical conformation	899:929	The triple-helical conformation	899:929	The triple-helical conformation was broken down into a random coil when the DS exceeded 0.34.
31733257	1	60	theme	sulfur	234:239	arg1	method					267:272	sulfur trioxide·pyridine complex method	234:272	sulfur trioxide·pyridine complex method	234:272	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	0	61	theme	polysaccharide	72:85	arg1	characterization					23:38	characterization	23:38	characterization	23:38	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	61	theme	polysaccharide	72:85	arg1	modification					9:20	modification	9:20	modification	9:20	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	0	61	theme	polysaccharide	72:85	arg1	bioactivities					55:67	potential bioactivities	45:67	potential bioactivities	45:67	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	9	62	theme	sulfated	1165:1172	arg1	derivatives					1174:1184	the five sulfated derivatives	1156:1184	the five sulfated derivatives	1156:1184	Among the five sulfated derivatives, SRVP1-25 exhibited the strongest ABTS and hydroxyl radical scavenging activities and anticoagulant activity, while SRVP1-20 showed the best antibacterial activity and anti-tumor activity.
31733257	2	63	theme	different	398:406	arg1	degrees					408:414	different degrees	398:414	different degrees of substitution (DS) ranging from 0.34 to 0.73	398:461	Five sulfated RVP derivatives (SRVP1-5, SRV1-10, SRVP1-15, SRVP1-20 and SRVP1-25) with different degrees of substitution (DS) ranging from 0.34 to 0.73 were prepared.
31733257	1	64	theme	trioxide·pyridine	241:257	arg1	method					267:272	sulfur trioxide·pyridine complex method	234:272	sulfur trioxide·pyridine complex method	234:272	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
31733257	5	65	theme	increasing	780:789	arg1	DS					791:792	the increasing DS	776:792	the increasing DS	776:792	The molecular weights of SRVP decreased with the increasing DS.
31733257	0	66	from	characterization	23:38	arg1	bodies					105:110	the fruiting bodies	92:110	the fruiting bodies of Russula virescens	92:131	Sulfated modification, characterization, and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens.
31733257	3	67	theme	SRVPs	517:521	arg1	features					497:504	Several structural features	478:504	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation	478:619	Several structural features of RVP and SRVPs including chemical structure, monosaccharide composition, molecular weight and chain conformation were investigated.
31733257	1	68	theme	complex	259:265	arg1	method					267:272	sulfur trioxide·pyridine complex method	234:272	sulfur trioxide·pyridine complex method	234:272	The water-soluble polysaccharide from fruiting bodies of Russula virescens (RVP) was sulfated using sulfur trioxide·pyridine complex method under different reaction conditions.
30849116	1	0	theme	membrane	178:185	arg1	potentials					187:196	membrane potentials	178:196	membrane potentials	178:196	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	9	1	with	glycosaminoglycans	1235:1252	arg1	sulfate					1306:1312	dermatan sulfate	1297:1312	dermatan sulfate	1297:1312	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	1	with	glycosaminoglycans	1235:1252	arg1	acid					1370:1373	hyaluronic acid	1359:1373	hyaluronic acid	1359:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	1	with	glycosaminoglycans	1235:1252	arg1	A					1335:1335	chondroitin sulfate A	1315:1335	chondroitin sulfate A	1315:1335	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	1	with	glycosaminoglycans	1235:1252	arg1	sulfate					1346:1352	heparan sulfate	1338:1352	heparan sulfate	1338:1352	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	1	with	glycosaminoglycans	1235:1252	arg1	structures					1276:1285	similar chemical structures	1259:1285	similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid	1259:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	2	theme	hyaluronic	1359:1368	arg1	acid					1370:1373	hyaluronic acid	1359:1373	hyaluronic acid	1359:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	5	3	theme	proton	792:797	arg1	substance					810:818	the highest naturally occurring proton conducting substance	760:818	the highest naturally occurring proton conducting substance	760:818	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	3	theme	proton	792:797	arg1	jelly					683:687	the jelly	679:687	the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs-	679:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	6	4	theme	keratan	928:934	arg1	KS					945:946	KS	945:946	KS	945:946	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	6	4	theme	keratan	928:934	arg1	sulfate					936:942	the glycosaminoglycan keratan sulfate	906:942	the glycosaminoglycan keratan sulfate (KS)	906:947	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	9	5	theme	Proton	1164:1169	arg1	conductivity					1171:1182	Proton conductivity	1164:1182	Proton conductivity	1164:1182	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	6	6	theme	complex	837:843	arg1	composition					845:855	a complex composition	835:855	a complex composition	835:855	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	8	7	theme	PdHx	1047:1050	arg1	contacts					1052:1059	PdHx contacts	1047:1059	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.	1047:1162	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	1	8	from	important	66:74	arg1	phenomena					92:100	many natural phenomena	79:100	many natural phenomena including oxidative phosphorylation in mitochondria and archaea	79:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	1	8	from	important	66:74	arg1	phosphorylation					122:136	oxidative phosphorylation	112:136	oxidative phosphorylation in mitochondria and archaea	112:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	5	9	theme	electro-sensing	733:747	arg1	organs-					749:755	Lorenzini- shark's electro-sensing organs-	714:755	Lorenzini- shark's electro-sensing organs-	714:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	6	10	theme	glycosaminoglycan	910:926	arg1	KS					945:946	KS	945:946	KS	945:946	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	6	10	theme	glycosaminoglycan	910:926	arg1	sulfate					936:942	the glycosaminoglycan keratan sulfate	906:942	the glycosaminoglycan keratan sulfate (KS)	906:947	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	7	11	theme	proton	970:975	arg1	conductivity					977:988	the proton conductivity	966:988	the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea	966:1044	Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea.
30849116	5	12	theme	organs-	749:755	arg1	Ampullae					702:709	the Ampullae	698:709	the Ampullae of Lorenzini- shark's electro-sensing organs-	698:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	13	theme	Lorenzini-	714:723	arg1	shark					725:729	Lorenzini- shark's	714:731	Lorenzini- shark's electro-sensing organs-	714:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	7	14	theme	Bovine	1032:1037	arg1	Cornea					1039:1044	Bovine Cornea	1032:1044	Bovine Cornea	1032:1044	Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea.
30849116	9	15	theme	sulfate	1327:1333	arg1	A					1335:1335	chondroitin sulfate A	1315:1335	chondroitin sulfate A	1315:1335	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	16	theme	similar	1259:1265	arg1	sulfate					1306:1312	dermatan sulfate	1297:1312	dermatan sulfate	1297:1312	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	16	theme	similar	1259:1265	arg1	acid					1370:1373	hyaluronic acid	1359:1373	hyaluronic acid	1359:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	16	theme	similar	1259:1265	arg1	A					1335:1335	chondroitin sulfate A	1315:1335	chondroitin sulfate A	1315:1335	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	16	theme	similar	1259:1265	arg1	sulfate					1346:1352	heparan sulfate	1338:1352	heparan sulfate	1338:1352	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	16	theme	similar	1259:1265	arg1	structures					1276:1285	similar chemical structures	1259:1285	similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid	1259:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	1	17	theme	many	79:82	arg1	phenomena					92:100	many natural phenomena	79:100	many natural phenomena including oxidative phosphorylation in mitochondria and archaea	79:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	1	17	theme	many	79:82	arg1	phosphorylation					122:136	oxidative phosphorylation	112:136	oxidative phosphorylation in mitochondria and archaea	112:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	10	18	theme	proton	1427:1432	arg1	conductivity					1434:1445	proton conductivity	1427:1445	proton conductivity	1427:1445	This observation supports the relationship between proton conductivity and the chemical structure of biopolymers.
30849116	9	19	theme	chemical	1267:1274	arg1	sulfate					1306:1312	dermatan sulfate	1297:1312	dermatan sulfate	1297:1312	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	19	theme	chemical	1267:1274	arg1	acid					1370:1373	hyaluronic acid	1359:1373	hyaluronic acid	1359:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	19	theme	chemical	1267:1274	arg1	A					1335:1335	chondroitin sulfate A	1315:1335	chondroitin sulfate A	1315:1335	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	19	theme	chemical	1267:1274	arg1	sulfate					1346:1352	heparan sulfate	1338:1352	heparan sulfate	1338:1352	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	9	19	theme	chemical	1267:1274	arg1	structures					1276:1285	similar chemical structures	1259:1285	similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid	1259:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	5	20	theme	conducting	799:808	arg1	substance					810:818	the highest naturally occurring proton conducting substance	760:818	the highest naturally occurring proton conducting substance	760:818	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	20	theme	conducting	799:808	arg1	jelly					683:687	the jelly	679:687	the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs-	679:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	1	21	theme	natural	84:90	arg1	phenomena					92:100	many natural phenomena	79:100	many natural phenomena including oxidative phosphorylation in mitochondria and archaea	79:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	1	21	theme	natural	84:90	arg1	phosphorylation					122:136	oxidative phosphorylation	112:136	oxidative phosphorylation in mitochondria and archaea	112:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	10	22	theme	chemical	1455:1462	arg1	structure					1464:1472	the chemical structure	1451:1472	the chemical structure of biopolymers	1451:1487	This observation supports the relationship between proton conductivity and the chemical structure of biopolymers.
30849116	3	23	theme	many	465:468	arg1	biopolymers					479:489	many hydrated biopolymers	465:489	many hydrated biopolymers	465:489	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	23	theme	many	465:468	arg1	keratin					529:535	keratin	529:535	keratin	529:535	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	23	theme	many	465:468	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	23	theme	many	465:468	arg1	collagen					519:526	collagen	519:526	collagen	519:526	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	23	theme	many	465:468	arg1	chitosan					538:545	chitosan	538:545	chitosan	538:545	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	8	24	theme	±	1150:1150	arg1	cm					1157:1158	2 ± 1 mS cm	1148:1158	2 ± 1 mS cm -1	1148:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	8	25	theme	0.11	1071:1074	arg1	cm					1079:1080	0.50 ± 0.11 mS cm -1	1064:1083	0.50 ± 0.11 mS cm -1	1064:1083	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	1	26	theme	antibiotic	205:214	arg1	Gramicidin					216:225	the antibiotic Gramicidin, and proton actuated bioluminescence	201:262	Gramicidin	216:225	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	0	27	theme	Proton	0:5	arg1	conductivity					7:18	Proton conductivity	0:18	Proton conductivity of glycosaminoglycans.	0:41	Proton conductivity of glycosaminoglycans.
30849116	8	28	theme	±	1069:1069	arg1	cm					1079:1080	0.50 ± 0.11 mS cm -1	1064:1083	0.50 ± 0.11 mS cm -1	1064:1083	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	3	29	located	present	454:460	arg1	chitosan					538:545	chitosan	538:545	chitosan	538:545	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg1	collagen					519:526	collagen	519:526	collagen	519:526	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg1	keratin					529:535	keratin	529:535	keratin	529:535	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg2	chains					420:425	These chains	414:425	These chains of hydrogen bonds	414:443	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg1	biopolymers					479:489	many hydrated biopolymers	465:489	many hydrated biopolymers	465:489	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	29	located	present	454:460	arg1	macromolecule					495:507	macromolecule	495:507	macromolecule	495:507	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	8	30	from	Ampullae	1117:1124	arg1	cm					1157:1158	2 ± 1 mS cm	1148:1158	2 ± 1 mS cm -1	1148:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	0	31	theme	glycosaminoglycans	23:40	arg1	conductivity					7:18	Proton conductivity	0:18	Proton conductivity of glycosaminoglycans.	0:41	Proton conductivity of glycosaminoglycans.
30849116	3	32	attach	present	454:460	arg1	chitosan					538:545	chitosan	538:545	chitosan	538:545	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg1	collagen					519:526	collagen	519:526	collagen	519:526	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg1	keratin					529:535	keratin	529:535	keratin	529:535	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg2	chains					420:425	These chains	414:425	These chains of hydrogen bonds	414:443	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg1	biopolymers					479:489	many hydrated biopolymers	465:489	many hydrated biopolymers	465:489	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	32	attach	present	454:460	arg1	macromolecule					495:507	macromolecule	495:507	macromolecule	495:507	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	2	33	theme	protons	328:334	arg1	conduction					314:323	the conduction	310:323	the conduction of protons	310:334	In all of these phenomena, the conduction of protons occurs along chains of hydrogen bonds between water and hydrophilic residues.
30849116	5	34	located	found	689:693	arg2	jelly					683:687	the jelly	679:687	the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs-	679:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	34	located	found	689:693	arg1	Ampullae					702:709	the Ampullae	698:709	the Ampullae of Lorenzini- shark's electro-sensing organs-	698:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	34	located	found	689:693	arg2	substance					810:818	the highest naturally occurring proton conducting substance	760:818	the highest naturally occurring proton conducting substance	760:818	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	3	35	from	present	454:460	arg1	chitosan					538:545	chitosan	538:545	chitosan	538:545	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	35	from	present	454:460	arg1	collagen					519:526	collagen	519:526	collagen	519:526	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	35	from	present	454:460	arg1	keratin					529:535	keratin	529:535	keratin	529:535	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	35	from	present	454:460	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	35	from	present	454:460	arg1	biopolymers					479:489	many hydrated biopolymers	465:489	many hydrated biopolymers	465:489	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	35	from	present	454:460	arg1	macromolecule					495:507	macromolecule	495:507	macromolecule	495:507	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	36	theme	various	552:558	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	36	theme	various	552:558	arg1	reflectin					577:585	reflectin	577:585	reflectin	577:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	9	37	theme	lower	1197:1201	arg1	values					1203:1208	lower values	1197:1208	lower values	1197:1208	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	3	38	theme	bonds	439:443	arg1	chains					420:425	These chains	414:425	These chains of hydrogen bonds	414:443	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	5	39	dep	highest	764:770	arg1	occurring					782:790	occurring	782:790	occurring	782:790	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	3	40	theme	hydrated	470:477	arg1	biopolymers					479:489	many hydrated biopolymers	465:489	many hydrated biopolymers	465:489	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	40	theme	hydrated	470:477	arg1	keratin					529:535	keratin	529:535	keratin	529:535	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	40	theme	hydrated	470:477	arg1	proteins					560:567	various proteins	552:567	various proteins such as reflectin	552:585	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	40	theme	hydrated	470:477	arg1	collagen					519:526	collagen	519:526	collagen	519:526	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	3	40	theme	hydrated	470:477	arg1	chitosan					538:545	chitosan	538:545	chitosan	538:545	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	8	41	theme	mS	1076:1077	arg1	cm					1079:1080	0.50 ± 0.11 mS cm -1	1064:1083	0.50 ± 0.11 mS cm -1	1064:1083	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	1	42	theme	oxidative	112:120	arg1	phosphorylation					122:136	oxidative phosphorylation	112:136	oxidative phosphorylation in mitochondria and archaea	112:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	1	43	theme	proton	232:237	arg1	bioluminescence					248:262	the antibiotic Gramicidin, and proton actuated bioluminescence	201:262	bioluminescence	248:262	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	9	44	theme	chondroitin	1315:1325	arg1	sulfate					1327:1333	chondroitin sulfate	1315:1333	chondroitin sulfate A	1315:1335	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	1	45	theme	actuated	239:246	arg1	bioluminescence					248:262	the antibiotic Gramicidin, and proton actuated bioluminescence	201:262	bioluminescence	248:262	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	8	46	theme	jelly	1139:1143	arg1	Ampullae					1117:1124	Ampullae	1117:1124	Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1	1117:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	9	47	theme	dermatan	1297:1304	arg1	sulfate					1306:1312	dermatan sulfate	1297:1312	dermatan sulfate	1297:1312	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	3	48	from	biopolymers	479:489	arg1	present					454:460	present	454:460	present	454:460	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	2	49	theme	bonds	368:372	arg1	chains					349:354	chains	349:354	chains of hydrogen bonds between water and hydrophilic residues	349:411	In all of these phenomena, the conduction of protons occurs along chains of hydrogen bonds between water and hydrophilic residues.
30849116	3	50	theme	hydrogen	430:437	arg1	bonds					439:443	hydrogen bonds	430:443	hydrogen bonds	430:443	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	7	51	theme	sulfate	1010:1016	arg1	conductivity					977:988	the proton conductivity	966:988	the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea	966:1044	Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea.
30849116	2	52	theme	hydrogen	359:366	arg1	bonds					368:372	hydrogen bonds	359:372	hydrogen bonds between water and hydrophilic residues	359:411	In all of these phenomena, the conduction of protons occurs along chains of hydrogen bonds between water and hydrophilic residues.
30849116	7	53	theme	keratan	1002:1008	arg1	sulfate					1010:1016	hydrated keratan sulfate	993:1016	hydrated keratan sulfate purified from Bovine Cornea	993:1044	Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea.
30849116	1	54	from	bioluminescence	248:262	arg1	dinoflagellate					267:280	dinoflagellate	267:280	dinoflagellate	267:280	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	3	55	from	macromolecule	495:507	arg1	present					454:460	present	454:460	present	454:460	These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin.
30849116	1	56	from	phosphorylation	122:136	arg1	archaea					158:164	archaea	158:164	archaea	158:164	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	1	56	from	phosphorylation	122:136	arg1	mitochondria					141:152	mitochondria	141:152	mitochondria	141:152	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	9	57	theme	heparan	1338:1344	arg1	sulfate					1346:1352	heparan sulfate	1338:1352	heparan sulfate	1338:1352	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	10	58	theme	biopolymers	1477:1487	arg1	structure					1464:1472	the chemical structure	1451:1472	the chemical structure of biopolymers	1451:1487	This observation supports the relationship between proton conductivity and the chemical structure of biopolymers.
30849116	10	58	theme	biopolymers	1477:1487	arg1	conductivity					1434:1445	proton conductivity	1427:1445	proton conductivity	1427:1445	This observation supports the relationship between proton conductivity and the chemical structure of biopolymers.
30849116	1	59	theme	Proton	43:48	arg1	conductivity					50:61	Proton conductivity	43:61	Proton conductivity	43:61	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	7	60	theme	hydrated	993:1000	arg1	sulfate					1010:1016	hydrated keratan sulfate	993:1016	hydrated keratan sulfate purified from Bovine Cornea	993:1044	Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea.
30849116	1	61	from	phenomena	92:100	arg1	important					66:74	important	66:74	important	66:74	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	4	62	theme	proton	620:625	arg1	conductors					627:636	proton conductors	620:636	proton conductors	620:636	All of these materials are also proton conductors.
30849116	4	62	theme	proton	620:625	arg1	All					588:590	All	588:590	All	588:590	All of these materials are also proton conductors.
30849116	4	62	theme	proton	620:625	arg1	materials					601:609	these materials	595:609	these materials	595:609	All of these materials are also proton conductors.
30849116	5	63	theme	highest	764:770	arg1	substance					810:818	the highest naturally occurring proton conducting substance	760:818	the highest naturally occurring proton conducting substance	760:818	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	5	63	theme	highest	764:770	arg1	jelly					683:687	the jelly	679:687	the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs-	679:755	Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini- shark's electro-sensing organs- is the highest naturally occurring proton conducting substance.
30849116	1	64	from	Gramicidin	216:225	arg1	dinoflagellate					267:280	dinoflagellate	267:280	dinoflagellate	267:280	Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate.
30849116	2	65	theme	hydrophilic	392:402	arg1	residues					404:411	hydrophilic residues	392:411	hydrophilic residues	392:411	In all of these phenomena, the conduction of protons occurs along chains of hydrogen bonds between water and hydrophilic residues.
30849116	9	66	theme	other	1229:1233	arg1	glycosaminoglycans					1235:1252	other glycosaminoglycans	1229:1252	other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid	1229:1373	Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid.
30849116	8	67	from	cm	1079:1080	arg1	contacts					1052:1059	PdHx contacts	1047:1059	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.	1047:1162	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	6	68	contain	has	831:833	arg2	composition					845:855	a complex composition	835:855	a complex composition	835:855	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	6	68	contain	has	831:833	arg1	jelly					825:829	The jelly	821:829	The jelly	821:829	The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS).
30849116	8	69	from	cm	1157:1158	arg1	Ampullae					1117:1124	Ampullae	1117:1124	Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1	1117:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	8	69	from	cm	1157:1158	arg1	jelly					1139:1143	Lorenzini jelly	1129:1143	Lorenzini jelly at 2 ± 1 mS cm -1	1129:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	8	70	theme	mS	1154:1155	arg1	cm					1157:1158	2 ± 1 mS cm	1148:1158	2 ± 1 mS cm -1	1148:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
30849116	8	71	theme	Lorenzini	1129:1137	arg1	jelly					1139:1143	Lorenzini jelly	1129:1143	Lorenzini jelly at 2 ± 1 mS cm -1	1129:1161	PdHx contacts at 0.50 ± 0.11 mS cm -1, which is consistent to that of Ampullae of Lorenzini jelly at 2 ± 1 mS cm -1.
31421200	8	0	contain	have	1328:1331	arg2	effect					1336:1341	an effect	1333:1341	an effect	1333:1341	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	8	0	contain	have	1328:1331	arg1	speed					1314:1318	The rotation speed	1301:1318	The rotation speed	1301:1318	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	7	1	theme	Main	1043:1046	arg1	compaction					1048:1057	Main compaction	1043:1057	Main compaction	1043:1057	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	7	1	theme	Main	1043:1046	arg1	parameter					1077:1085	the principal parameter	1063:1085	the principal parameter leading to delamination	1063:1109	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	1	2	theme	process	153:159	arg1	pressures					236:244	main compaction pressures	220:244	main compaction pressures	220:244	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	2	theme	process	153:159	arg1	pre-compaction					201:214	pre-compaction	201:214	pre-compaction	201:214	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	2	theme	process	153:159	arg1	parameters					161:170	tableting process parameters	143:170	tableting process parameters	143:170	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	7	3	theme	turret	1278:1283	arg1	rotation					1285:1292	turret rotation	1278:1292	turret rotation speed	1278:1298	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	2	4	used	used	350:353	arg2	recovery					320:327	The elastic recovery	308:327	The elastic recovery after compaction	308:344	The elastic recovery after compaction was used as estimation for the elasticity of the material.
31421200	2	4	used	used	350:353	arg2	estimation					358:367	estimation	358:367	estimation for the elasticity of the material	358:402	The elastic recovery after compaction was used as estimation for the elasticity of the material.
31421200	1	5	theme	parameters	161:170	arg1	influence					130:138	the influence	126:138	the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets	126:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	7	6	theme	excipients	1228:1237	arg1	elasticity					1214:1223	elasticity	1214:1223	elasticity of excipients	1214:1237	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	7	7	theme	high	1175:1178	arg1	pressures					1180:1188	high pressures	1175:1188	high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed	1175:1298	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	3	8	theme	controlled	577:586	arg1	excipients					601:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients	483:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	483:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	9	theme	interactions	257:268	arg1	influence					130:138	the influence	126:138	the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets	126:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	2	10	theme	elastic	312:318	arg1	recovery					320:327	The elastic recovery	308:327	The elastic recovery after compaction	308:344	The elastic recovery after compaction was used as estimation for the elasticity of the material.
31421200	2	10	theme	elastic	312:318	arg1	estimation					358:367	estimation	358:367	estimation for the elasticity of the material	358:402	The elastic recovery after compaction was used as estimation for the elasticity of the material.
31421200	8	11	theme	formulations	1376:1387	arg1	case					1368:1371	the case	1364:1371	the case of formulations with brittle excipients	1364:1411	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	4	12	theme	Experiment	751:760	arg1	Design					741:746	A 3-levels 3-factors central composite Design	702:746	A 3-levels 3-factors central composite Design of Experiment	702:760	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	8	13	theme	layer	1346:1350	arg1	adhesion					1352:1359	layer adhesion	1346:1359	layer adhesion in the case of formulations with brittle excipients	1346:1411	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	6	14	theme	turret	941:946	arg1	compaction					926:935	Main compaction	921:935	Main compaction	921:935	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	14	theme	turret	941:946	arg1	rotation					948:955	turret rotation	941:955	turret rotation	941:955	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	14	theme	turret	941:946	arg1	parameters					987:996	the most important parameters	968:996	the most important parameters	968:996	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	7	15	with	formulations	1122:1133	arg1	materials					1148:1156	plastic materials	1140:1156	plastic materials	1140:1156	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	3	16	theme	pharmaceutical	421:434	arg1	combinations					467:478	combinations	467:478	combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	467:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	16	theme	pharmaceutical	421:434	arg1	formulations					436:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	17	dep	excipients	601:610	arg1	cellulose					619:627	ethyl cellulose	613:627	ethyl cellulose	613:627	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	17	dep	excipients	601:610	arg1	methylcellulose					644:658	hydroxypropyl methylcellulose	630:658	hydroxypropyl methylcellulose	630:658	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	17	dep	excipients	601:610	arg1	acetate/polyvinylpyrrolidone					671:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	5	18	theme	shear	853:857	arg1	test					859:862	A custom-made shear test	839:862	A custom-made shear test	839:862	A custom-made shear test was used to determine the tablet tendency to delaminate.
31421200	8	19	theme	brittle	1394:1400	arg1	excipients					1402:1411	brittle excipients	1394:1411	brittle excipients	1394:1411	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	7	20	contain	had	1239:1241	arg2	impact					1251:1256	a major impact	1243:1256	a major impact	1243:1256	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	7	20	contain	had	1239:1241	arg1	difference					1200:1209	the difference	1196:1209	the difference in elasticity of excipients	1196:1237	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	3	21	theme	pregelatinized	550:563	arg1	starch					565:570	pregelatinized starch	550:570	pregelatinized starch	550:570	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	21	theme	pregelatinized	550:563	arg1	cellulose					511:519	microcrystalline cellulose	494:519	microcrystalline cellulose	494:519	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	22	from	influence	130:138	arg1	adhesion					279:286	layer adhesion	273:286	layer adhesion of bilayer tablets	273:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	3	23	theme	drug	588:591	arg1	excipients					601:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients	483:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	483:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	24	theme	turret	178:183	arg1	rotation					185:192	turret rotation	178:192	turret rotation speed	178:198	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	7	25	theme	major	1245:1249	arg1	impact					1251:1256	a major impact	1243:1256	a major impact	1243:1256	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	1	26	theme	layer	273:277	arg1	adhesion					279:286	layer adhesion	273:286	layer adhesion of bilayer tablets	273:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	6	27	theme	important	977:985	arg1	compaction					926:935	Main compaction	921:935	Main compaction	921:935	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	27	theme	important	977:985	arg1	rotation					948:955	turret rotation	941:955	turret rotation	941:955	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	27	theme	important	977:985	arg1	parameters					987:996	the most important parameters	968:996	the most important parameters	968:996	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	4	28	theme	3-levels	704:711	arg1	Design					741:746	A 3-levels 3-factors central composite Design	702:746	A 3-levels 3-factors central composite Design of Experiment	702:760	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	3	29	theme	release	593:599	arg1	excipients					601:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients	483:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	483:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	4	30	theme	3-factors	713:721	arg1	Design					741:746	A 3-levels 3-factors central composite Design	702:746	A 3-levels 3-factors central composite Design of Experiment	702:760	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	0	31	theme	process	28:34	arg1	parameters					36:45	manufacturing process parameters	14:45	manufacturing process parameters	14:45	Evaluation of manufacturing process parameters causing multilayer tablets delamination.
31421200	6	32	theme	tablet	1021:1026	arg1	manufacturing					1028:1040	tablet manufacturing	1021:1040	tablet manufacturing	1021:1040	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	1	33	theme	study	104:108	arg1	aim					92:94	The aim	88:94	The aim of this study	88:108	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	8	34	from	adhesion	1352:1359	arg1	case					1368:1371	the case	1364:1371	the case of formulations with brittle excipients	1364:1411	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	0	35	theme	manufacturing	14:26	arg1	parameters					36:45	manufacturing process parameters	14:45	manufacturing process parameters	14:45	Evaluation of manufacturing process parameters causing multilayer tablets delamination.
31421200	7	36	theme	plastic	1140:1146	arg1	materials					1148:1156	plastic materials	1140:1156	plastic materials	1140:1156	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	6	37	theme	Main	921:924	arg1	compaction					926:935	Main compaction	921:935	Main compaction	921:935	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	37	theme	Main	921:924	arg1	rotation					948:955	turret rotation	941:955	turret rotation	941:955	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	6	37	theme	Main	921:924	arg1	parameters					987:996	the most important parameters	968:996	the most important parameters	968:996	Main compaction and turret rotation speed were the most important parameters to be optimized during tablet manufacturing.
31421200	0	38	dep	delamination	74:85	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of manufacturing process parameters causing multilayer tablets	0:72	Evaluation of manufacturing process parameters causing multilayer tablets delamination.
31421200	3	39	theme	immediate	483:491	arg1	excipients					601:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients	483:610	immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	483:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	40	theme	bilayer	291:297	arg1	tablets					299:305	bilayer tablets	291:305	bilayer tablets	291:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	3	41	theme	ethyl	613:617	arg1	cellulose					619:627	ethyl cellulose	613:627	ethyl cellulose	613:627	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	41	theme	ethyl	613:617	arg1	methylcellulose					644:658	hydroxypropyl methylcellulose	630:658	hydroxypropyl methylcellulose	630:658	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	41	theme	ethyl	613:617	arg1	acetate/polyvinylpyrrolidone					671:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	8	42	theme	rotation	1305:1312	arg1	speed					1314:1318	The rotation speed	1301:1318	The rotation speed	1301:1318	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	5	43	theme	tablet	890:895	arg1	tendency					897:904	the tablet tendency	886:904	the tablet tendency to delaminate	886:918	A custom-made shear test was used to determine the tablet tendency to delaminate.
31421200	0	44	theme	parameters	36:45	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of manufacturing process parameters causing multilayer tablets	0:72	Evaluation of manufacturing process parameters causing multilayer tablets delamination.
31421200	1	45	theme	tablets	299:305	arg1	adhesion					279:286	layer adhesion	273:286	layer adhesion of bilayer tablets	273:305	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	3	46	theme	potential	411:419	arg1	combinations					467:478	combinations	467:478	combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	467:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	46	theme	potential	411:419	arg1	formulations					436:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	47	theme	polyvinyl	661:669	arg1	cellulose					619:627	ethyl cellulose	613:627	ethyl cellulose	613:627	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	47	theme	polyvinyl	661:669	arg1	acetate/polyvinylpyrrolidone					671:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	polyvinyl acetate/polyvinylpyrrolidone	661:698	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	48	dep	rotation	185:192	arg1	i.e.					173:176	i.e.	173:176	i.e.	173:176	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	8	49	with	formulations	1376:1387	arg1	excipients					1402:1411	brittle excipients	1394:1411	brittle excipients	1394:1411	The rotation speed did not have an effect on layer adhesion in the case of formulations with brittle excipients.
31421200	2	50	theme	material	395:402	arg1	elasticity					377:386	the elasticity	373:386	the elasticity of the material	373:402	The elastic recovery after compaction was used as estimation for the elasticity of the material.
31421200	5	51	theme	custom-made	841:851	arg1	test					859:862	A custom-made shear test	839:862	A custom-made shear test	839:862	A custom-made shear test was used to determine the tablet tendency to delaminate.
31421200	4	52	theme	central	723:729	arg1	Design					741:746	A 3-levels 3-factors central composite Design	702:746	A 3-levels 3-factors central composite Design of Experiment	702:760	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	3	53	theme	excipients	601:610	arg1	combinations					467:478	combinations	467:478	combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone)	467:699	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	53	theme	excipients	601:610	arg1	formulations					436:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations	405:447	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	0	54	theme	multilayer	55:64	arg1	tablets					66:72	multilayer tablets	55:72	multilayer tablets	55:72	Evaluation of manufacturing process parameters causing multilayer tablets delamination.
31421200	5	55	used	used	868:871	arg2	test					859:862	A custom-made shear test	839:862	A custom-made shear test	839:862	A custom-made shear test was used to determine the tablet tendency to delaminate.
31421200	3	56	dep	immediate	483:491	arg1	cellulose					511:519	microcrystalline cellulose	494:519	microcrystalline cellulose	494:519	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	56	dep	immediate	483:491	arg1	starch					565:570	pregelatinized starch	550:570	pregelatinized starch	550:570	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	56	dep	immediate	483:491	arg1	lactose					522:528	lactose	522:528	lactose	522:528	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	56	dep	immediate	483:491	arg1	phosphate					539:547	calcium phosphate	531:547	calcium phosphate	531:547	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	4	57	theme	composite	731:739	arg1	Design					741:746	A 3-levels 3-factors central composite Design	702:746	A 3-levels 3-factors central composite Design of Experiment	702:760	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	4	58	theme	layer	802:806	arg1	adhesion					808:815	layer adhesion	802:815	layer adhesion selected as response	802:836	A 3-levels 3-factors central composite Design of Experiment was performed on each formulation, with layer adhesion selected as response.
31421200	1	59	theme	main	220:223	arg1	pressures					236:244	main compaction pressures	220:244	main compaction pressures	220:244	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	59	theme	main	220:223	arg1	parameters					161:170	tableting process parameters	143:170	tableting process parameters	143:170	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	7	60	theme	principal	1067:1075	arg1	compaction					1048:1057	Main compaction	1043:1057	Main compaction	1043:1057	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	7	60	theme	principal	1067:1075	arg1	parameter					1077:1085	the principal parameter	1063:1085	the principal parameter leading to delamination	1063:1109	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	3	61	theme	microcrystalline	494:509	arg1	cellulose					511:519	microcrystalline cellulose	494:519	microcrystalline cellulose	494:519	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	61	theme	microcrystalline	494:509	arg1	starch					565:570	pregelatinized starch	550:570	pregelatinized starch	550:570	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	61	theme	microcrystalline	494:509	arg1	lactose					522:528	lactose	522:528	lactose	522:528	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	61	theme	microcrystalline	494:509	arg1	phosphate					539:547	calcium phosphate	531:547	calcium phosphate	531:547	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	62	theme	compaction	225:234	arg1	pressures					236:244	main compaction pressures	220:244	main compaction pressures	220:244	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	62	theme	compaction	225:234	arg1	parameters					161:170	tableting process parameters	143:170	tableting process parameters	143:170	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	3	63	theme	calcium	531:537	arg1	cellulose					511:519	microcrystalline cellulose	494:519	microcrystalline cellulose	494:519	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	63	theme	calcium	531:537	arg1	phosphate					539:547	calcium phosphate	531:547	calcium phosphate	531:547	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	7	64	from	difference	1200:1209	arg1	elasticity					1214:1223	elasticity	1214:1223	elasticity of excipients	1214:1237	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	3	65	theme	hydroxypropyl	630:642	arg1	cellulose					619:627	ethyl cellulose	613:627	ethyl cellulose	613:627	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	3	65	theme	hydroxypropyl	630:642	arg1	methylcellulose					644:658	hydroxypropyl methylcellulose	630:658	hydroxypropyl methylcellulose	630:658	Three potential pharmaceutical formulations were evaluated as combinations of immediate (microcrystalline cellulose, lactose, calcium phosphate, pregelatinized starch) and controlled drug release excipients (ethyl cellulose, hydroxypropyl methylcellulose, polyvinyl acetate/polyvinylpyrrolidone).
31421200	1	66	theme	tableting	143:151	arg1	pressures					236:244	main compaction pressures	220:244	main compaction pressures	220:244	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	66	theme	tableting	143:151	arg1	pre-compaction					201:214	pre-compaction	201:214	pre-compaction	201:214	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	1	66	theme	tableting	143:151	arg1	parameters					161:170	tableting process parameters	143:170	tableting process parameters	143:170	The aim of this study was to evaluate the influence of tableting process parameters, i.e. turret rotation speed, pre-compaction and main compaction pressures, and their interactions on layer adhesion of bilayer tablets.
31421200	7	67	from	pressures	1180:1188	arg1	compaction					1048:1057	Main compaction	1043:1057	Main compaction	1043:1057	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
31421200	7	67	from	pressures	1180:1188	arg1	parameter					1077:1085	the principal parameter	1063:1085	the principal parameter leading to delamination	1063:1109	Main compaction was the principal parameter leading to delamination in case of formulations with plastic materials, particularly at high pressures where the difference in elasticity of excipients had a major impact and was followed by turret rotation speed.
30347801	1	0	theme	breast	327:332	arg1	milk					334:337	breast milk	327:337	breast milk	327:337	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	1	1	theme	oligosaccharides	154:169	arg1	composition					177:187	human milk oligosaccharides (HMO) composition	143:187	human milk oligosaccharides (HMO) composition in breast milk	143:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	1	2	theme	preterm	236:242	arg1	infants					244:250	some preterm infants	231:250	some preterm infants	231:250	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	7	3	theme	3'-sialyllactose	1149:1164	arg1	sepsis					1206:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	2	4	theme	weight	551:556	arg1	infants					574:580	extremely low birth weight (ELBW; <1000 g) infants	531:580	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	7	5	theme	culture-proven	1191:1204	arg1	sepsis					1206:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	8	6	from	variation	1319:1327	arg1	composition					1336:1346	HMO composition	1332:1346	HMO composition	1332:1346	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	8	6	from	variation	1319:1327	arg1	milk					1358:1361	breast milk	1351:1361	breast milk	1351:1361	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	0	7	theme	Low	103:105	arg1	Infants					120:126	Extremely Low Birth Weight Infants	93:126	Extremely Low Birth Weight Infants	93:126	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	2	8	theme	birth	545:549	arg1	weight					551:556	extremely low birth weight	531:556	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	2	8	theme	birth	545:549	arg1	g					571:571	ELBW; <1000 g	559:571	ELBW; <1000 g	559:571	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	2	9	from	concentration	381:393	arg1	milk					425:428	breast milk	418:428	breast milk	418:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	2	10	theme	low	541:543	arg1	weight					551:556	extremely low birth weight	531:556	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	2	10	theme	low	541:543	arg1	g					571:571	ELBW; <1000 g	559:571	ELBW; <1000 g	559:571	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	5	11	theme	sampling	980:987	arg1	points					994:999	all sampling time points	976:999	all sampling time points	976:999	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	1	12	theme	necrotizing	260:270	arg1	NEC					287:289	NEC	287:289	NEC	287:289	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	1	12	theme	necrotizing	260:270	arg1	enterocolitis					272:284	necrotizing enterocolitis	260:284	necrotizing enterocolitis (NEC)	260:290	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	0	13	theme	Weight	113:118	arg1	Infants					120:126	Extremely Low Birth Weight Infants	93:126	Extremely Low Birth Weight Infants	93:126	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	2	14	from	milk	425:428	arg1	concentration					381:393	the concentration	377:393	the concentration of 15 dominant HMOs in breast milk	377:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	6	15	theme	Lacto-N-difucohexaose	1002:1022	arg1	I					1024:1024	Lacto-N-difucohexaose I	1002:1024	Lacto-N-difucohexaose I	1002:1024	Lacto-N-difucohexaose I is only produced by secretor and Lewis positive mothers.
30347801	0	16	theme	Birth	107:111	arg1	Infants					120:126	Extremely Low Birth Weight Infants	93:126	Extremely Low Birth Weight Infants	93:126	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	5	17	from	points	994:999	arg1	cases					967:971	non-NEC cases	959:971	non-NEC cases at all sampling time points	959:999	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	2	18	theme	neonatal	441:448	arg1	period					450:455	the neonatal period	437:455	the neonatal period	437:455	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	2	19	theme	breast	612:617	arg1	milk					619:622	breast milk	612:622	breast milk	612:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	7	20	theme	significant	1099:1109	arg1	associations					1128:1139	significant but inconsistent associations	1099:1139	significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate	1099:1287	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	2	21	theme	breast	418:423	arg1	milk					425:428	breast milk	418:428	breast milk	418:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	4	22	theme	high-performance	751:766	arg1	chromatography					783:796	high-performance anion-exchange chromatography	751:796	high-performance anion-exchange chromatography with pulsed amperometric detection	751:831	The HMOs were analysed with high-performance anion-exchange chromatography with pulsed amperometric detection.
30347801	5	23	from	mothers	922:928	arg1	samples					909:915	samples	909:915	samples from mothers to NEC cases	909:941	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	0	24	theme	Low	0:2	arg1	Diversity					4:12	Low Diversity	0:12	Low Diversity of Human Milk Oligosaccharides	0:43	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	6	25	theme	secretor	1046:1053	arg1	mothers					1074:1080	secretor and Lewis positive mothers	1046:1080	secretor and Lewis positive mothers	1046:1080	Lacto-N-difucohexaose I is only produced by secretor and Lewis positive mothers.
30347801	6	26	theme	positive	1065:1072	arg1	mothers					1074:1080	secretor and Lewis positive mothers	1046:1080	secretor and Lewis positive mothers	1046:1080	Lacto-N-difucohexaose I is only produced by secretor and Lewis positive mothers.
30347801	2	27	theme	study	356:360	arg1	aim					344:346	The aim	340:346	The aim of this study	340:360	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	0	28	theme	Human	17:21	arg1	Oligosaccharides					28:43	Human Milk Oligosaccharides	17:43	Human Milk Oligosaccharides	17:43	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	8	29	dep	fed	1429:1431	arg1	milk					1424:1427	breast milk	1417:1427	breast milk	1417:1427	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	8	30	theme	breast	1351:1356	arg1	milk					1358:1361	breast milk	1351:1361	breast milk	1351:1361	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	4	31	theme	anion-exchange	768:781	arg1	chromatography					783:796	high-performance anion-exchange chromatography	751:796	high-performance anion-exchange chromatography with pulsed amperometric detection	751:831	The HMOs were analysed with high-performance anion-exchange chromatography with pulsed amperometric detection.
30347801	1	32	theme	breast	192:197	arg1	milk					199:202	breast milk	192:202	breast milk	192:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	2	33	theme	dominant	401:408	arg1	HMOs					410:413	15 dominant HMOs	398:413	15 dominant HMOs in breast milk	398:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	8	34	theme	fed	1429:1431	arg1	infants					1438:1444	exclusively breast milk fed ELBW infants	1405:1444	exclusively breast milk fed ELBW infants	1405:1444	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	0	35	theme	Oligosaccharides	28:43	arg1	Diversity					4:12	Low Diversity	0:12	Low Diversity of Human Milk Oligosaccharides	0:43	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	7	36	theme	weak	1234:1237	arg1	correlations					1239:1250	significant, but weak correlations	1217:1250	significant, but weak correlations between several HMOs and growth rate	1217:1287	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	0	37	from	Enterocolitis	76:88	arg1	Infants					120:126	Extremely Low Birth Weight Infants	93:126	Extremely Low Birth Weight Infants	93:126	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	2	38	from	growth	521:526	arg1	infants					574:580	extremely low birth weight (ELBW; <1000 g) infants	531:580	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	7	39	theme	growth	1277:1282	arg1	rate					1284:1287	growth rate	1277:1287	growth rate	1277:1287	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	0	40	theme	Milk	23:26	arg1	Oligosaccharides					28:43	Human Milk Oligosaccharides	17:43	Human Milk Oligosaccharides	17:43	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	5	41	theme	time	989:992	arg1	points					994:999	all sampling time points	976:999	all sampling time points	976:999	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	2	42	theme	HMOs	410:413	arg1	concentration					381:393	the concentration	377:393	the concentration of 15 dominant HMOs in breast milk	377:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	7	43	theme	inconsistent	1115:1126	arg1	associations					1128:1139	significant but inconsistent associations	1099:1139	significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate	1099:1287	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	5	44	theme	HMOs	838:841	arg1	diversity					843:851	The HMOs diversity	834:851	The HMOs diversity	834:851	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	5	44	theme	HMOs	838:841	arg1	lower					900:904	lower	900:904	lower	900:904	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	4	45	theme	amperometric	810:821	arg1	detection					823:831	pulsed amperometric detection	803:831	pulsed amperometric detection	803:831	The HMOs were analysed with high-performance anion-exchange chromatography with pulsed amperometric detection.
30347801	5	46	theme	non-NEC	959:965	arg1	cases					967:971	non-NEC cases	959:971	non-NEC cases at all sampling time points	959:999	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	4	47	theme	pulsed	803:808	arg1	detection					823:831	pulsed amperometric detection	803:831	pulsed amperometric detection	803:831	The HMOs were analysed with high-performance anion-exchange chromatography with pulsed amperometric detection.
30347801	1	48	from	Difference	129:138	arg1	composition					177:187	human milk oligosaccharides (HMO) composition	143:187	human milk oligosaccharides (HMO) composition in breast milk	143:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	7	49	theme	significant	1217:1227	arg1	correlations					1239:1250	significant, but weak correlations	1217:1250	significant, but weak correlations between several HMOs and growth rate	1217:1287	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	5	50	theme	Lacto-N-difucohexaose	871:891	arg1	diversity					843:851	The HMOs diversity	834:851	The HMOs diversity	834:851	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	5	50	theme	Lacto-N-difucohexaose	871:891	arg1	levels					861:866	the levels	857:866	the levels of Lacto-N-difucohexaose I	857:893	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	5	50	theme	Lacto-N-difucohexaose	871:891	arg1	lower					900:904	lower	900:904	lower	900:904	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	3	51	theme	postmenstrual	700:712	arg1	week					714:717	postmenstrual week 36	700:720	postmenstrual week 36	700:720	Milk was collected from 91 mothers to 106 infants at 14 and 28 days and at postmenstrual week 36.
30347801	8	52	theme	ELBW	1433:1436	arg1	infants					1438:1444	exclusively breast milk fed ELBW infants	1405:1444	exclusively breast milk fed ELBW infants	1405:1444	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	1	53	from	composition	177:187	arg1	milk					199:202	breast milk	192:202	breast milk	192:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	8	54	theme	breast	1417:1422	arg1	milk					1424:1427	breast milk	1417:1427	breast milk	1417:1427	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	4	55	with	chromatography	783:796	arg1	detection					823:831	pulsed amperometric detection	803:831	pulsed amperometric detection	803:831	The HMOs were analysed with high-performance anion-exchange chromatography with pulsed amperometric detection.
30347801	2	56	from	NEC	504:506	arg1	infants					574:580	extremely low birth weight (ELBW; <1000 g) infants	531:580	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	0	57	theme	Necrotising	64:74	arg1	Enterocolitis					76:88	Necrotising Enterocolitis	64:88	Necrotising Enterocolitis in Extremely Low Birth Weight Infants	64:126	Low Diversity of Human Milk Oligosaccharides is Associated with Necrotising Enterocolitis in Extremely Low Birth Weight Infants.
30347801	8	58	theme	HMO	1332:1334	arg1	composition					1336:1346	HMO composition	1332:1346	HMO composition	1332:1346	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	8	59	theme	important	1373:1381	arg1	variation					1319:1327	the variation	1315:1327	the variation in HMO composition in breast milk	1315:1361	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	8	59	theme	important	1373:1381	arg1	factor					1383:1388	an important factor	1370:1388	an important factor	1370:1388	Our results suggest that the variation in HMO composition in breast milk may be an important factor explaining why exclusively breast milk fed ELBW infants develop NEC.
30347801	7	60	theme	several	1260:1266	arg1	HMOs					1268:1271	several HMOs	1260:1271	several HMOs	1260:1271	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	1	61	theme	human	143:147	arg1	HMO					172:174	HMO	172:174	HMO	172:174	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	1	61	theme	human	143:147	arg1	oligosaccharides					154:169	human milk oligosaccharides	143:169	human milk oligosaccharides (HMO) composition in breast milk	143:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	7	62	theme	6'-sialyllactose	1170:1185	arg1	sepsis					1206:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis	1149:1211	There were also significant but inconsistent associations between 3'-sialyllactose and 6'-sialyllactose and culture-proven sepsis and significant, but weak correlations between several HMOs and growth rate.
30347801	2	63	from	HMOs	410:413	arg1	milk					425:428	breast milk	418:428	breast milk	418:428	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30347801	5	64	theme	NEC	933:935	arg1	cases					937:941	NEC cases	933:941	NEC cases	933:941	The HMOs diversity and the levels of Lacto-N-difucohexaose I were lower in samples from mothers to NEC cases, as compared to non-NEC cases at all sampling time points.
30347801	1	65	theme	milk	149:152	arg1	HMO					172:174	HMO	172:174	HMO	172:174	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	1	65	theme	milk	149:152	arg1	oligosaccharides					154:169	human milk oligosaccharides	143:169	human milk oligosaccharides (HMO) composition in breast milk	143:202	Difference in human milk oligosaccharides (HMO) composition in breast milk may be one explanation why some preterm infants develop necrotizing enterocolitis (NEC) despite being fed exclusively with breast milk.
30347801	2	66	from	sepsis	509:514	arg1	infants					574:580	extremely low birth weight (ELBW; <1000 g) infants	531:580	extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk	531:622	The aim of this study was to measure the concentration of 15 dominant HMOs in breast milk during the neonatal period and investigate how their levels correlated to NEC, sepsis, and growth in extremely low birth weight (ELBW; <1000 g) infants who were exclusively fed with breast milk.
30450908	0	0	theme	Intelligent	98:108	arg1	Packaging					115:123	Intelligent Food Packaging	98:123	Intelligent Food Packaging	98:123	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	1	1	theme	electrochemical	172:186	arg1	writing					188:194	electrochemical writing	172:194	electrochemical writing	172:194	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	1	2	theme	anthocyanins	255:266	arg1	extract					268:274	anthocyanins extract	255:274	anthocyanins extract	255:274	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	6	3	theme	written	886:892	arg1	patterns					894:901	written patterns	886:901	written patterns	886:901	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	5	4	theme	gas	712:714	arg1	films					690:694	The composite films	676:694	The composite films	676:694	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	5	4	theme	gas	712:714	arg1	sensors					716:722	gas sensors	712:722	gas sensors	712:722	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	3	5	theme	red	539:541	arg1	anthocyanins					550:561	red radish anthocyanins	539:561	red radish anthocyanins	539:561	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	6	6	theme	food	973:976	arg1	spoilage					978:985	food spoilage	973:985	food spoilage	973:985	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	6	6	theme	food	973:976	arg1	part					992:995	a part	990:995	a part of an intelligent packaging system	990:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	0	7	theme	Food	110:113	arg1	Packaging					115:123	Intelligent Food Packaging	98:123	Intelligent Food Packaging	98:123	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	2	8	from	change	364:369	arg1	range					381:385	the pH range	374:385	the pH range of 2-12	374:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	6	9	with	film	876:879	arg1	patterns					894:901	written patterns	886:901	written patterns	886:901	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	1	10	theme	intelligent	280:290	arg1	packaging					297:305	intelligent food packaging	280:305	intelligent food packaging	280:305	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	6	11	theme	composite	866:874	arg1	indicator					927:935	an easy-to-use indicator	912:935	an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system	912:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	6	11	theme	composite	866:874	arg1	film					876:879	this composite film	861:879	this composite film with written patterns	861:901	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	6	12	theme	system	1025:1030	arg1	spoilage					978:985	food spoilage	973:985	food spoilage	973:985	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	6	12	theme	system	1025:1030	arg1	part					992:995	a part	990:995	a part of an intelligent packaging system	990:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	3	13	theme	barrier	433:439	arg1	abilities					441:449	The tensile strength, ductility, and barrier abilities	396:449	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films	396:499	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	4	14	theme	Multicolor	574:583	arg1	patterns					585:592	Multicolor patterns	574:592	Multicolor patterns	574:592	Multicolor patterns were successfully drawn on the films by using the electrochemical writing method.
30450908	5	15	dep	milk	776:779	arg1	spoilage					790:797	spoilage	790:797	spoilage	790:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	5	16	theme	fish	785:788	arg1	presence					764:771	the presence	760:771	the presence of milk and fish spoilage	760:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	0	17	theme	Biomaterial-Based	8:24	arg1	Films					50:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	3	18	theme	radish	543:548	arg1	anthocyanins					550:561	red radish anthocyanins	539:561	red radish anthocyanins	539:561	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	2	19	theme	2-12	390:393	arg1	range					381:385	the pH range	374:385	the pH range of 2-12	374:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	0	20	theme	Natural	0:6	arg1	Films					50:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	1	21	theme	food	292:295	arg1	packaging					297:305	intelligent food packaging	280:305	intelligent food packaging	280:305	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	4	22	theme	electrochemical	644:658	arg1	method					668:673	the electrochemical writing method	640:673	the electrochemical writing method	640:673	Multicolor patterns were successfully drawn on the films by using the electrochemical writing method.
30450908	3	23	theme	anthocyanins	550:561	arg1	concentration					522:534	the concentration	518:534	the concentration of red radish anthocyanins	518:561	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	4	24	theme	writing	660:666	arg1	method					668:673	the electrochemical writing method	640:673	the electrochemical writing method	640:673	Multicolor patterns were successfully drawn on the films by using the electrochemical writing method.
30450908	6	25	theme	packaging	1015:1023	arg1	system					1025:1030	an intelligent packaging system	1000:1030	an intelligent packaging system	1000:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	0	26	theme	Edible	26:31	arg1	Films					50:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	6	27	theme	great	942:946	arg1	potential					948:956	great potential	942:956	great potential	942:956	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	2	28	theme	composite	312:320	arg1	film					322:325	The composite film	308:325	The composite film	308:325	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	3	29	theme	UV	467:468	arg1	light					471:475	ultraviolet (UV) light	454:475	ultraviolet (UV) light	454:475	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	5	30	theme	written	810:816	arg1	well-preserved					832:845	well-preserved	832:845	well-preserved	832:845	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	5	30	theme	written	810:816	arg1	patterns					818:825	the written patterns	806:825	the written patterns	806:825	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	1	31	theme	gellan	228:233	arg1	gum					235:237	gellan gum	228:237	gellan gum	228:237	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	0	32	theme	pH-Sensitive	37:48	arg1	Films					50:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films	0:54	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	3	33	theme	ductility	418:426	arg1	abilities					441:449	The tensile strength, ductility, and barrier abilities	396:449	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films	396:499	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	3	34	theme	ultraviolet	454:464	arg1	light					471:475	ultraviolet (UV) light	454:475	ultraviolet (UV) light	454:475	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	3	35	theme	tensile	400:406	arg1	strength					408:415	tensile strength	400:415	tensile strength	400:415	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	6	36	theme	easy-to-use	915:925	arg1	film					876:879	this composite film	861:879	this composite film with written patterns	861:901	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	6	36	theme	easy-to-use	915:925	arg1	indicator					927:935	an easy-to-use indicator	912:935	an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system	912:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	5	37	theme	milk	776:779	arg1	presence					764:771	the presence	760:771	the presence of milk and fish spoilage	760:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	5	38	theme	color	743:747	arg1	changes					749:755	visible color changes	735:755	visible color changes in the presence of milk and fish spoilage	735:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	1	39	theme	edible	129:134	arg1	film					153:156	An edible and pH-sensitive film	126:156	An edible and pH-sensitive film combined with electrochemical writing	126:194	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	3	40	theme	strength	408:415	arg1	abilities					441:449	The tensile strength, ductility, and barrier abilities	396:449	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films	396:499	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	5	41	from	changes	749:755	arg1	presence					764:771	the presence	760:771	the presence of milk and fish spoilage	760:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	2	42	theme	color	358:362	arg1	change					364:369	an orange red-to-yellow color change	334:369	an orange red-to-yellow color change in the pH range of 2-12	334:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	5	43	theme	composite	680:688	arg1	films					690:694	The composite films	676:694	The composite films	676:694	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	5	43	theme	composite	680:688	arg1	sensors					716:722	gas sensors	712:722	gas sensors	712:722	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	1	44	theme	pH-sensitive	140:151	arg1	film					153:156	An edible and pH-sensitive film	126:156	An edible and pH-sensitive film combined with electrochemical writing	126:194	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	6	45	with	indicator	927:935	arg1	potential					948:956	great potential	942:956	great potential	942:956	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	2	46	theme	orange	337:342	arg1	change					364:369	an orange red-to-yellow color change	334:369	an orange red-to-yellow color change in the pH range of 2-12	334:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	2	47	theme	red-to-yellow	344:356	arg1	change					364:369	an orange red-to-yellow color change	334:369	an orange red-to-yellow color change in the pH range of 2-12	334:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
30450908	5	48	theme	visible	735:741	arg1	changes					749:755	visible color changes	735:755	visible color changes in the presence of milk and fish spoilage	735:797	The composite films, which acted as gas sensors, presented visible color changes in the presence of milk and fish spoilage, while the written patterns were well-preserved.
30450908	6	49	theme	intelligent	1003:1013	arg1	system					1025:1030	an intelligent packaging system	1000:1030	an intelligent packaging system	1000:1030	Accordingly, this composite film with written patterns could be an easy-to-use indicator with great potential for monitoring food spoilage as a part of an intelligent packaging system.
30450908	1	50	dep	gelatin	219:225	arg1	extract					268:274	anthocyanins extract	255:274	anthocyanins extract	255:274	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	3	51	theme	films	495:499	arg1	oxygen					481:486	oxygen	481:486	oxygen	481:486	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	3	51	theme	films	495:499	arg1	light					471:475	ultraviolet (UV) light	454:475	ultraviolet (UV) light	454:475	The tensile strength, ductility, and barrier abilities to ultraviolet (UV) light and oxygen of the films were improved as the concentration of red radish anthocyanins increased.
30450908	1	52	theme	red	244:246	arg1	radish					248:253	red radish	244:253	red radish	244:253	An edible and pH-sensitive film combined with electrochemical writing was developed by using gelatin, gellan gum, and red radish anthocyanins extract for intelligent food packaging.
30450908	0	53	theme	Electrochemical	70:84	arg1	Writing					86:92	Electrochemical Writing	70:92	Electrochemical Writing for Intelligent Food Packaging	70:123	Natural Biomaterial-Based Edible and pH-Sensitive Films Combined with Electrochemical Writing for Intelligent Food Packaging.
30450908	2	54	theme	pH	378:379	arg1	range					381:385	the pH range	374:385	the pH range of 2-12	374:393	The composite film showed an orange red-to-yellow color change in the pH range of 2-12.
32299578	0	0	theme	vinyl	80:84	arg1	starch/poly					68:78	improved starch/poly	59:78	improved starch/poly(vinyl alcohol) composites	59:104	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	0	0	theme	vinyl	80:84	arg1	alcohol					86:92	vinyl alcohol	80:92	vinyl alcohol	80:92	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	2	1	from	embedded	412:419	arg1	composites					466:475	starch/poly(vinyl alcohol) (St/PVA)-based composites	424:475	starch/poly(vinyl alcohol) (St/PVA)-based composites	424:475	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	4	2	theme	plasticizing	819:830	arg1	effect					832:837	no plasticizing effect	816:837	no plasticizing effect	816:837	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	4	3	theme	PFrec	855:859	arg1	addition					861:868	PFrec addition	855:868	PFrec addition	855:868	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	1	4	theme	triglyceride-based	138:155	arg1	by-product					157:166	a triglyceride-based by-product	136:166	a triglyceride-based by-product	136:166	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	5	5	theme	Solubility	871:880	arg1	determinations					882:895	Solubility determinations	871:895	Solubility determinations for the St/PVA-based composite films	871:932	Solubility determinations for the St/PVA-based composite films showed that hydrophobic PFrec increased the water resistance with at least 40 %.
32299578	3	6	theme	Td3	639:641	arg1	%					643:643	Td3 %	639:643	Td3 %	639:643	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	3	7	theme	blends	610:615	arg1	stability					590:598	the thermal stability	578:598	the thermal stability of St/PVA blends	578:615	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	2	8	used	used	298:301	arg2	oil					290:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	8	used	used	298:301	arg2	monomer					321:327	photo-reactive monomer	306:327	photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly)	306:519	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	3	9	theme	%	643:643	arg1	increase					627:634	an 18 ⁰C increase	618:634	an 18 ⁰C increase of Td3 %	618:643	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	1	10	dep	method	118:123	arg1	harness					128:134	harness	128:134	to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater	125:253	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	6	11	theme	hydrophilic	1137:1147	arg1	molecules					1153:1161	hydrophilic Gly molecules	1137:1161	hydrophilic Gly molecules	1137:1161	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	5	12	theme	hydrophobic	946:956	arg1	PFrec					958:962	hydrophobic PFrec	946:962	hydrophobic PFrec	946:962	Solubility determinations for the St/PVA-based composite films showed that hydrophobic PFrec increased the water resistance with at least 40 %.
32299578	2	13	theme	polymeric	377:385	arg1	network					387:393	a polymeric network	375:393	a polymeric network (PFrec)	375:401	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	13	theme	polymeric	377:385	arg1	PFrec					396:400	PFrec	396:400	PFrec	396:400	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	0	14	from	treatment	39:47	arg1	byproduct					13:21	a byproduct	11:21	a byproduct from wastewater treatment	11:47	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	4	15	theme	PFrec	733:737	arg1	association					718:728	association	718:728	association of PFrec with Gly	718:746	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	6	16	theme	good	1057:1060	arg1	dispersion					1062:1071	a good dispersion	1055:1071	a good dispersion of PFrec in the St/PVA network	1055:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	1	17	theme	chicken	179:185	arg1	traces					191:196	chicken fat traces	179:196	chicken fat traces	179:196	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	3	18	theme	PFrec-loaded	666:677	arg1	sample					679:684	PFrec-loaded sample	666:684	PFrec-loaded sample	666:684	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	6	19	theme	angle	1036:1040	arg1	measurements					1042:1053	contact angle measurements	1028:1053	contact angle measurements	1028:1053	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	1	20	theme	fat	187:189	arg1	traces					191:196	chicken fat traces	179:196	chicken fat traces	179:196	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	4	21	theme	Mechanical	687:696	arg1	tests					698:702	Mechanical tests	687:702	Mechanical tests	687:702	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	4	22	located	observed	843:850	arg1	addition					861:868	PFrec addition	855:868	PFrec addition	855:868	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	4	22	located	observed	843:850	arg2	effect					832:837	no plasticizing effect	816:837	no plasticizing effect	816:837	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	6	23	theme	contact	1028:1034	arg1	measurements					1042:1053	contact angle measurements	1028:1053	contact angle measurements	1028:1053	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	5	24	theme	composite	918:926	arg1	films					928:932	the St/PVA-based composite films	901:932	the St/PVA-based composite films	901:932	Solubility determinations for the St/PVA-based composite films showed that hydrophobic PFrec increased the water resistance with at least 40 %.
32299578	5	25	theme	St/PVA-based	905:916	arg1	films					928:932	the St/PVA-based composite films	901:932	the St/PVA-based composite films	901:932	Solubility determinations for the St/PVA-based composite films showed that hydrophobic PFrec increased the water resistance with at least 40 %.
32299578	6	26	theme	Gly	1149:1151	arg1	molecules					1153:1161	hydrophilic Gly molecules	1137:1161	hydrophilic Gly molecules	1137:1161	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	2	27	with	"	338:338	arg1	molecules					351:359	the grease molecules	340:359	the grease molecules	340:359	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	28	theme	photo-reactive	306:319	arg1	monomer					321:327	photo-reactive monomer	306:327	photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly)	306:519	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	28	theme	photo-reactive	306:319	arg1	oil					290:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	3	29	theme	St/PVA	603:608	arg1	blends					610:615	St/PVA blends	603:615	St/PVA blends	603:615	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	0	30	theme	improved	59:66	arg1	starch/poly					68:78	improved starch/poly	59:78	improved starch/poly(vinyl alcohol) composites	59:104	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	0	30	theme	improved	59:66	arg1	alcohol					86:92	vinyl alcohol	80:92	vinyl alcohol	80:92	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	6	31	theme	St/PVA	1089:1094	arg1	network					1096:1102	the St/PVA network	1085:1102	the St/PVA network	1085:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	0	32	theme	wastewater	28:37	arg1	treatment					39:47	wastewater treatment	28:47	wastewater treatment	28:47	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	2	33	from	composites	466:475	arg1	embedded					412:419	embedded	412:419	embedded	412:419	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	4	34	with	association	718:728	arg1	Gly					744:746	Gly	744:746	Gly	744:746	Mechanical tests revealed that association of PFrec with Gly improved the flexibility and also reinforced the systems, although, no plasticizing effect was observed at PFrec addition.
32299578	1	35	contain	containing	168:177	arg2	traces					191:196	chicken fat traces	179:196	chicken fat traces	179:196	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	1	35	contain	containing	168:177	arg1	by-product					157:166	a triglyceride-based by-product	136:166	a triglyceride-based by-product	136:166	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	2	36	theme	grease	344:349	arg1	molecules					351:359	the grease molecules	340:359	the grease molecules	340:359	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	37	theme	vinyl	436:440	arg1	alcohol					442:448	vinyl alcohol	436:448	vinyl alcohol	436:448	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	38	theme	enhanced	527:534	arg1	properties					536:545	enhanced properties	527:545	enhanced properties	527:545	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	6	39	from	network	1096:1102	arg1	dispersion					1062:1071	a good dispersion	1055:1071	a good dispersion of PFrec in the St/PVA network	1055:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	1	40	theme	rational	109:116	arg1	method					118:123	A rational method	107:123	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater	107:253	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	2	41	theme	linseed	282:288	arg1	monomer					321:327	photo-reactive monomer	306:327	photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly)	306:519	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	41	theme	linseed	282:288	arg1	oil					290:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	1	42	theme	simulated	218:226	arg1	wastewater					244:253	the simulated slaughterhouses wastewater	214:253	the simulated slaughterhouses wastewater	214:253	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	2	43	dep	-based	459:464	arg1	alcohol					442:448	vinyl alcohol	436:448	vinyl alcohol	436:448	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	44	theme	Methacrylated	268:280	arg1	monomer					321:327	photo-reactive monomer	306:327	photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly)	306:519	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	2	44	theme	Methacrylated	268:280	arg1	oil					290:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil	268:292	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	6	45	from	dispersion	1062:1071	arg1	network					1096:1102	the St/PVA network	1085:1102	the St/PVA network	1085:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	2	46	theme	catch	333:337	arg1	"					338:338	"catch"	332:338	"catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly)	332:519	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	1	47	theme	slaughterhouses	228:242	arg1	wastewater					244:253	the simulated slaughterhouses wastewater	214:253	the simulated slaughterhouses wastewater	214:253	A rational method to harness a triglyceride-based by-product containing chicken fat traces, extracted from the simulated slaughterhouses wastewater was adopted.
32299578	0	48	theme	starch/poly	68:78	arg1	composites					95:104	improved starch/poly(vinyl alcohol) composites	59:104	improved starch/poly(vinyl alcohol) composites	59:104	Harnessing a byproduct from wastewater treatment to obtain improved starch/poly(vinyl alcohol) composites.
32299578	3	49	theme	⁰C	624:625	arg1	increase					627:634	an 18 ⁰C increase	618:634	an 18 ⁰C increase of Td3 %	618:643	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	5	50	theme	water	978:982	arg1	resistance					984:993	the water resistance	974:993	the water resistance	974:993	Solubility determinations for the St/PVA-based composite films showed that hydrophobic PFrec increased the water resistance with at least 40 %.
32299578	6	51	theme	PFrec	1076:1080	arg1	dispersion					1062:1071	a good dispersion	1055:1071	a good dispersion of PFrec in the St/PVA network	1055:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
32299578	2	52	theme	-based	459:464	arg1	composites					466:475	starch/poly(vinyl alcohol) (St/PVA)-based composites	424:475	starch/poly(vinyl alcohol) (St/PVA)-based composites	424:475	Methacrylated linseed oil was used as photo-reactive monomer to "catch" the grease molecules, resulting in a polymeric network (PFrec), further embedded in starch/poly(vinyl alcohol) (St/PVA)-based composites, with or without plasticizer (glycerol-Gly), with enhanced properties.
32299578	3	53	theme	thermal	582:588	arg1	stability					590:598	the thermal stability	578:598	the thermal stability of St/PVA blends	578:615	Hydrophobic additive improved the thermal stability of St/PVA blends, an 18 ⁰C increase of Td3 % being registered for PFrec-loaded sample.
32299578	6	54	from	PFrec	1076:1080	arg1	network					1096:1102	the St/PVA network	1085:1102	the St/PVA network	1085:1102	According to contact angle measurements a good dispersion of PFrec in the St/PVA network was mediated at the interface by hydrophilic Gly molecules.
30236648	1	0	theme	NPs	341:343	arg1	dispersions					296:306	liquid dispersions	289:306	liquid dispersions of polyelectrolyte complex (PEC) NPs	289:343	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	11	1	theme	Caco-2	1278:1283	arg1	cells					1285:1289	Caco-2 cells	1278:1289	Caco-2 cells	1278:1289	PEG polymers were well tolerated by Caco-2 cells, with the exception of linear PEG 10 kDa.
30236648	12	2	theme	content	1481:1487	arg1	optimisation					1437:1448	optimisation	1437:1448	optimisation of the cryoprotectant type and content	1437:1487	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	3	from	loading	1407:1413	arg1	product					1428:1434	the dried product	1418:1434	the dried product	1418:1434	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	7	4	theme	PEC	869:871	arg1	NPs					873:875	PEC NPs	869:875	PEC NPs	869:875	The concentration of TRE was optimised for each type of PEC NPs.
30236648	0	5	theme	composition	79:89	arg1	Effect					56:61	Effect	56:61	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.	0:119	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	1	6	theme	NP	172:173	arg1	composition					176:186	nanoparticle (NP) composition	158:186	nanoparticle (NP) composition	158:186	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	0	7	theme	selection	110:118	arg1	Effect					56:61	Effect	56:61	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.	0:119	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	6	8	theme	powder	790:795	arg1	reconstitution					797:810	a successful powder reconstitution	777:810	a successful powder reconstitution	777:810	The presence of a cryoprotectant was essential to achieve a successful powder reconstitution.
30236648	3	9	theme	ethylene	537:544	arg1	poly					532:535	poly	532:535	poly(ethylene glycol) (PEG)	532:558	The cryo-/lyo-protectants investigated were mannitol, trehalose (TRE) and poly(ethylene glycol) (PEG).
30236648	3	9	theme	ethylene	537:544	arg1	glycol					546:551	ethylene glycol	537:551	ethylene glycol	537:551	The cryo-/lyo-protectants investigated were mannitol, trehalose (TRE) and poly(ethylene glycol) (PEG).
30236648	0	10	theme	cryoprotectant	95:108	arg1	selection					110:118	cryoprotectant selection	95:118	cryoprotectant selection	95:118	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	1	11	theme	redispersible	361:373	arg1	powders					375:381	completely redispersible powders	350:381	completely redispersible powders	350:381	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	9	12	theme	NPs	1128:1130	arg1	drying					1118:1123	co-freeze drying	1108:1123	co-freeze drying of NPs with TRE and PEG	1108:1147	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	6	13	theme	successful	779:788	arg1	reconstitution					797:810	a successful powder reconstitution	777:810	a successful powder reconstitution	777:810	The presence of a cryoprotectant was essential to achieve a successful powder reconstitution.
30236648	12	14	from	ratio	1604:1608	arg1	PEC					1577:1579	the PEC	1573:1579	the PEC	1573:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	15	theme	polyions	1595:1602	arg1	ratio					1604:1608	the polyions ratio	1591:1608	the polyions ratio	1591:1608	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	4	16	theme	solid	565:569	arg1	state					571:575	The solid state	561:575	The solid state of lyophilised powders	561:598	The solid state of lyophilised powders was studied by thermal analysis and X-ray diffraction.
30236648	12	17	theme	maximising	1393:1402	arg1	loading					1407:1413	maximising NP loading	1393:1413	maximising NP loading	1393:1413	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	7	18	theme	NPs	873:875	arg1	type					861:864	each type	856:864	each type of PEC NPs	856:875	The concentration of TRE was optimised for each type of PEC NPs.
30236648	4	19	theme	X-ray	636:640	arg1	diffraction					642:652	X-ray diffraction	636:652	X-ray diffraction	636:652	The solid state of lyophilised powders was studied by thermal analysis and X-ray diffraction.
30236648	0	20	theme	Freeze	0:5	arg1	drying					7:12	Freeze drying	0:12	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.	0:119	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	7	21	theme	TRE	834:836	arg1	concentration					817:829	The concentration	813:829	The concentration of TRE	813:836	The concentration of TRE was optimised for each type of PEC NPs.
30236648	1	22	theme	composition	176:186	arg1	impact					148:153	the impact	144:153	the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants	144:229	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	11	23	theme	PEG	1321:1323	arg1	10 kDa					1325:1330	linear PEG 10 kDa	1314:1330	linear PEG 10 kDa	1314:1330	PEG polymers were well tolerated by Caco-2 cells, with the exception of linear PEG 10 kDa.
30236648	12	24	theme	NP	1404:1405	arg1	loading					1407:1413	maximising NP loading	1393:1413	maximising NP loading	1393:1413	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	2	25	theme	complex	436:442	arg1	coacervation					444:455	complex coacervation	436:455	complex coacervation	436:455	PEC NPs, with and without peptide, were produced by complex coacervation.
30236648	12	26	theme	pair	1565:1568	arg1	type					1541:1544	the type	1537:1544	the type of polyelectrolyte pair in the PEC	1537:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	26	theme	pair	1565:1568	arg1	ratio					1604:1608	the polyions ratio	1591:1608	the polyions ratio	1591:1608	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	11	27	theme	10 kDa	1325:1330	arg1	exception					1301:1309	the exception	1297:1309	the exception of linear PEG 10 kDa	1297:1330	PEG polymers were well tolerated by Caco-2 cells, with the exception of linear PEG 10 kDa.
30236648	5	28	theme	flow	703:706	arg1	cytometry					708:716	flow cytometry	703:716	flow cytometry	703:716	Cytotoxicity studies were done by MTS assay and flow cytometry.
30236648	1	29	theme	effectiveness	192:204	arg1	impact					148:153	the impact	144:153	the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants	144:229	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	0	30	theme	polyelectrolyte	17:31	arg1	nanoparticles					41:53	polyelectrolyte complex nanoparticles	17:53	polyelectrolyte complex nanoparticles	17:53	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	1	31	theme	liquid	289:294	arg1	dispersions					296:306	liquid dispersions	289:306	liquid dispersions of polyelectrolyte complex (PEC) NPs	289:343	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	11	32	theme	linear	1314:1319	arg1	10 kDa					1325:1330	linear PEG 10 kDa	1314:1330	linear PEG 10 kDa	1314:1330	PEG polymers were well tolerated by Caco-2 cells, with the exception of linear PEG 10 kDa.
30236648	4	33	theme	lyophilised	580:590	arg1	powders					592:598	lyophilised powders	580:598	lyophilised powders	580:598	The solid state of lyophilised powders was studied by thermal analysis and X-ray diffraction.
30236648	12	34	from	PEC	1577:1579	arg1	type					1541:1544	the type	1537:1544	the type of polyelectrolyte pair in the PEC	1537:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	34	from	PEC	1577:1579	arg1	ratio					1604:1608	the polyions ratio	1591:1608	the polyions ratio	1591:1608	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	9	35	theme	co-freeze	1108:1116	arg1	drying					1118:1123	co-freeze drying	1108:1123	co-freeze drying of NPs with TRE and PEG	1108:1147	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	1	36	theme	cryo-/lyo-protectants	209:229	arg1	effectiveness					192:204	effectiveness	192:204	effectiveness	192:204	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	1	36	theme	cryo-/lyo-protectants	209:229	arg1	composition					176:186	nanoparticle (NP) composition	158:186	nanoparticle (NP) composition	158:186	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	0	37	theme	nanoparticles	41:53	arg1	drying					7:12	Freeze drying	0:12	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.	0:119	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	9	38	theme	best	1075:1078	arg1	results					1080:1086	best results	1075:1086	best results	1075:1086	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	10	39	theme	storage	1223:1229	arg1	stability					1231:1239	better storage stability	1216:1239	better storage stability	1216:1239	These ternary NPs/TRE/PEG samples were crystalline, with expected better storage stability.
30236648	9	40	with	drying	1118:1123	arg1	PEG					1145:1147	PEG	1145:1147	PEG	1145:1147	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	9	40	with	drying	1118:1123	arg1	TRE					1137:1139	TRE	1137:1139	TRE	1137:1139	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	8	41	theme	Protamine-	878:887	arg1	NPs					911:913	Protamine- and hyaluronate-based NPs	878:913	Protamine- and hyaluronate-based NPs	878:913	Protamine- and hyaluronate-based NPs reconstituted better than chitosan- and chondroitin sulphate-based NPs, respectively.
30236648	0	42	theme	complex	33:39	arg1	nanoparticles					41:53	polyelectrolyte complex nanoparticles	17:53	polyelectrolyte complex nanoparticles	17:53	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	1	43	theme	polyelectrolyte	311:325	arg1	NPs					341:343	polyelectrolyte complex (PEC) NPs	311:343	polyelectrolyte complex (PEC) NPs	311:343	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	1	43	theme	polyelectrolyte	311:325	arg1	PEC					336:338	PEC	336:338	PEC	336:338	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	9	44	theme	PEG	1001:1003	arg1	polymers					1005:1012	PEG polymers	1001:1012	PEG polymers	1001:1012	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	6	45	theme	cryoprotectant	737:750	arg1	essential					756:764	essential	756:764	essential	756:764	The presence of a cryoprotectant was essential to achieve a successful powder reconstitution.
30236648	6	45	theme	cryoprotectant	737:750	arg1	presence					723:730	The presence	719:730	The presence of a cryoprotectant	719:750	The presence of a cryoprotectant was essential to achieve a successful powder reconstitution.
30236648	4	46	theme	thermal	615:621	arg1	analysis					623:630	thermal analysis	615:630	thermal analysis	615:630	The solid state of lyophilised powders was studied by thermal analysis and X-ray diffraction.
30236648	12	47	from	pair	1565:1568	arg1	PEC					1577:1579	the PEC	1573:1579	the PEC	1573:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	1	48	theme	complex	327:333	arg1	NPs					341:343	polyelectrolyte complex (PEC) NPs	311:343	polyelectrolyte complex (PEC) NPs	311:343	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	1	48	theme	complex	327:333	arg1	PEC					336:338	PEC	336:338	PEC	336:338	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	5	49	theme	Cytotoxicity	655:666	arg1	studies					668:674	Cytotoxicity studies	655:674	Cytotoxicity studies	655:674	Cytotoxicity studies were done by MTS assay and flow cytometry.
30236648	12	50	theme	polyelectrolyte	1549:1563	arg1	pair					1565:1568	polyelectrolyte pair	1549:1568	polyelectrolyte pair in the PEC	1549:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	8	51	theme	hyaluronate-based	893:909	arg1	NPs					911:913	Protamine- and hyaluronate-based NPs	878:913	Protamine- and hyaluronate-based NPs	878:913	Protamine- and hyaluronate-based NPs reconstituted better than chitosan- and chondroitin sulphate-based NPs, respectively.
30236648	12	52	dep	design	1382:1387	arg1	the					1366:1368	the	1366:1368	the	1366:1368	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	53	from	type	1541:1544	arg1	PEC					1577:1579	the PEC	1573:1579	the PEC	1573:1579	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	8	54	theme	better	929:934	arg1	NPs					982:984	better than chitosan- and chondroitin sulphate-based NPs	929:984	better than chitosan- and chondroitin sulphate-based NPs	929:984	Protamine- and hyaluronate-based NPs reconstituted better than chitosan- and chondroitin sulphate-based NPs, respectively.
30236648	12	55	theme	type	1472:1475	arg1	optimisation					1437:1448	optimisation	1437:1448	optimisation of the cryoprotectant type and content	1437:1487	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	11	56	theme	PEG	1242:1244	arg1	polymers					1246:1253	PEG polymers	1242:1253	PEG polymers	1242:1253	PEG polymers were well tolerated by Caco-2 cells, with the exception of linear PEG 10 kDa.
30236648	8	57	theme	sulphate-based	967:980	arg1	NPs					982:984	better than chitosan- and chondroitin sulphate-based NPs	929:984	better than chitosan- and chondroitin sulphate-based NPs	929:984	Protamine- and hyaluronate-based NPs reconstituted better than chitosan- and chondroitin sulphate-based NPs, respectively.
30236648	1	58	theme	freeze	236:241	arg1	process					250:256	a freeze drying process	234:256	a freeze drying process	234:256	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	12	59	theme	cryoprotectant	1457:1470	arg1	type					1472:1475	the cryoprotectant type	1453:1475	the cryoprotectant type	1453:1475	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	60	theme	formulation	1370:1380	arg1	design					1382:1387	formulation design	1370:1387	formulation design	1370:1387	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	12	61	from	design	1382:1387	arg1	product					1428:1434	the dried product	1418:1434	the dried product	1418:1434	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	5	62	theme	MTS	689:691	arg1	assay					693:697	MTS assay	689:697	MTS assay	689:697	Cytotoxicity studies were done by MTS assay and flow cytometry.
30236648	1	63	theme	drying	243:248	arg1	process					250:256	a freeze drying process	234:256	a freeze drying process	234:256	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	0	64	theme	nanoparticle	66:77	arg1	composition					79:89	nanoparticle composition	66:89	nanoparticle composition	66:89	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	0	65	dep	drying	7:12	arg1	Effect					56:61	Effect	56:61	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.	0:119	Freeze drying of polyelectrolyte complex nanoparticles: Effect of nanoparticle composition and cryoprotectant selection.
30236648	2	66	theme	PEC	384:386	arg1	NPs					388:390	PEC NPs	384:390	PEC NPs	384:390	PEC NPs, with and without peptide, were produced by complex coacervation.
30236648	10	67	theme	better	1216:1221	arg1	stability					1231:1239	better storage stability	1216:1239	better storage stability	1216:1239	These ternary NPs/TRE/PEG samples were crystalline, with expected better storage stability.
30236648	10	68	theme	NPs/TRE/PEG	1164:1174	arg1	samples					1176:1182	These ternary NPs/TRE/PEG samples	1150:1182	These ternary NPs/TRE/PEG samples	1150:1182	These ternary NPs/TRE/PEG samples were crystalline, with expected better storage stability.
30236648	12	69	theme	dried	1422:1426	arg1	product					1428:1434	the dried product	1418:1434	the dried product	1418:1434	This work shows that, as regards the formulation design and maximising NP loading in the dried product, optimisation of the cryoprotectant type and content is needed as it is highly dependent not only on the type of polyelectrolyte pair in the PEC, but also the polyions ratio.
30236648	10	70	theme	ternary	1156:1162	arg1	samples					1176:1182	These ternary NPs/TRE/PEG samples	1150:1182	These ternary NPs/TRE/PEG samples	1150:1182	These ternary NPs/TRE/PEG samples were crystalline, with expected better storage stability.
30236648	1	71	theme	nanoparticle	158:169	arg1	composition					176:186	nanoparticle (NP) composition	158:186	nanoparticle (NP) composition	158:186	This work investigates the impact of nanoparticle (NP) composition and effectiveness of cryo-/lyo-protectants in a freeze drying process, which was employed to convert liquid dispersions of polyelectrolyte complex (PEC) NPs into completely redispersible powders.
30236648	9	72	theme	effective	1036:1044	arg1	cryoprotectants					1046:1060	more effective cryoprotectants	1031:1060	more effective cryoprotectants than TRE	1031:1069	PEG polymers were found to be more effective cryoprotectants than TRE and best results were achieved using co-freeze drying of NPs with TRE and PEG.
30236648	4	73	theme	powders	592:598	arg1	state					571:575	The solid state	561:575	The solid state of lyophilised powders	561:598	The solid state of lyophilised powders was studied by thermal analysis and X-ray diffraction.
29549749	2	0	theme	charges	468:474	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	1	theme	systematic	341:350	arg1	study					352:356	a systematic study	339:356	a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	339:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	2	theme	new	1021:1023	arg1	insight					1025:1031	new insight	1021:1031	new insight into the surface charge effects on Aβ aggregation	1021:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	0	3	theme	β-protein	94:102	arg1	aggregation					104:114	amyloid β-protein aggregation	86:114	amyloid β-protein aggregation	86:114	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	3	4	theme	charges	653:659	arg1	density					661:667	the surface charges density	641:667	the surface charges density	641:667	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	2	5	theme	zeta	489:492	arg1	to -35 mV					514:522	zeta potentials from +38 to -35 mV	489:522	zeta potentials from +38 to -35 mV	489:522	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	5	theme	zeta	489:492	arg1	CH1					477:479	CH1	477:479	CH1 to CH7	477:486	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	6	from	values	807:812	arg1	Studies					783:789	Studies	783:789	Studies at different pH values	783:812	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	5	7	from	effects	1057:1063	arg1	aggregation					1071:1081	Aβ aggregation	1068:1081	Aβ aggregation	1068:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	5	8	theme	pH	804:805	arg1	values					807:812	different pH values	794:812	different pH values	794:812	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	4	9	theme	Circular	670:677	arg1	spectroscopy					689:700	Circular dichroism spectroscopy	670:700	Circular dichroism spectroscopy	670:700	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	5	10	theme	Aβ	1068:1069	arg1	aggregation					1071:1081	Aβ aggregation	1068:1081	Aβ aggregation	1068:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	4	11	from	difference	743:752	arg1	mechanisms					771:780	their working mechanisms	757:780	their working mechanisms	757:780	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	4	12	theme	working	763:769	arg1	mechanisms					771:780	their working mechanisms	757:780	their working mechanisms	757:780	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	5	13	theme	electrostatic	850:862	arg1	interactions					864:875	electrostatic interactions	850:875	electrostatic interactions	850:875	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	2	14	theme	different	450:458	arg1	charges					468:474	different surface charges	450:474	different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	450:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	15	theme	Aβ	965:966	arg1	property					975:982	Aβ charge property	965:982	Aβ charge property	965:982	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	5	16	theme	interactions	864:875	arg1	importance					836:845	the importance	832:845	the importance of electrostatic interactions in Aβ aggregation	832:893	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	2	17	theme	CH	439:440	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	18	theme	surface	460:466	arg1	charges					468:474	different surface charges	450:474	different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	450:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	19	theme	NPs	932:934	arg1	effects					918:924	the effects	914:924	the effects of CH NPs	914:934	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	0	20	theme	Charge	0:5	arg1	effects					7:13	Charge effects	0:13	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.	0:115	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	1	21	theme	Amyloid	117:123	arg1	aggregation					140:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	5	22	dep	pH.	989:991	arg1	provided					1012:1019	provided	1012:1019	has thus provided new insight into the surface charge effects on Aβ aggregation	1003:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	3	23	dep	NPs	560:562	arg1	Both					526:529	Both	526:529	Both	526:529	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	0	24	theme	self-assembled	18:31	arg1	nanoparticles					58:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	4	25	theme	force	713:717	arg1	microscopy					719:728	atomic force microscopy	706:728	atomic force microscopy	706:728	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	2	26	from	study	352:356	arg1	issue					365:369	the issue	361:369	the issue	361:369	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	1	27	theme	β-protein	125:133	arg1	aggregation					140:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	2	28	theme	chitosan-hyaluronic	403:421	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	29	theme	acid	423:426	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	1	30	theme	important	276:284	arg1	factor					286:291	an important factor	273:291	an important factor influencing Aβ aggregation	273:318	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	1	30	theme	important	276:284	arg1	charge					220:225	surface charge	212:225	surface charge of nanoparticles (NPs)	212:248	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	2	31	theme	self-assembled	388:401	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	0	32	theme	acid	53:56	arg1	nanoparticles					58:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	5	33	theme	surface	1042:1048	arg1	effects					1057:1063	the surface charge effects	1038:1063	the surface charge effects on Aβ aggregation	1038:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	3	34	theme	CH	557:558	arg1	NPs					560:562	Both the positive and negative CH NPs	526:562	NPs	560:562	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	5	35	theme	Aβ	880:881	arg1	aggregation					883:893	Aβ aggregation	880:893	Aβ aggregation	880:893	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	1	36	theme	surface	212:218	arg1	factor					286:291	an important factor	273:291	an important factor influencing Aβ aggregation	273:318	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	1	36	theme	surface	212:218	arg1	charge					220:225	surface charge	212:225	surface charge of nanoparticles (NPs)	212:248	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	0	37	theme	chitosan-hyaluronic	33:51	arg1	nanoparticles					58:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	self-assembled chitosan-hyaluronic acid nanoparticles	18:70	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	2	38	theme	potentials	494:503	arg1	to -35 mV					514:522	zeta potentials from +38 to -35 mV	489:522	zeta potentials from +38 to -35 mV	489:522	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	38	theme	potentials	494:503	arg1	CH1					477:479	CH1	477:479	CH1 to CH7	477:486	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	39	theme	charge	1050:1055	arg1	effects					1057:1063	the surface charge effects	1038:1063	the surface charge effects on Aβ aggregation	1038:1081	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	2	40	theme	composite	428:436	arg1	NPs					443:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs	388:445	self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	388:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	5	41	from	importance	836:845	arg1	aggregation					883:893	Aβ aggregation	880:893	Aβ aggregation	880:893	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	2	42	theme	NPs	443:445	arg1	series					378:383	a series	376:383	a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	376:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	1	43	theme	Aβ	305:306	arg1	aggregation					308:318	Aβ aggregation	305:318	Aβ aggregation	305:318	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	2	44	dep	charges	468:474	arg1	to -35 mV					514:522	zeta potentials from +38 to -35 mV	489:522	zeta potentials from +38 to -35 mV	489:522	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	2	44	dep	charges	468:474	arg1	CH1					477:479	CH1	477:479	CH1 to CH7	477:486	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	3	45	theme	inhibitory	597:606	arg1	effect					608:613	the inhibitory effect	593:613	the inhibitory effect	593:613	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	1	46	theme	Aβ	136:137	arg1	aggregation					140:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation	117:150	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	0	47	theme	nanoparticles	58:70	arg1	effects					7:13	Charge effects	0:13	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.	0:115	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	3	48	theme	negative	548:555	arg1	NPs					560:562	Both the positive and negative CH NPs	526:562	NPs	560:562	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	4	49	theme	dichroism	679:687	arg1	spectroscopy					689:700	Circular dichroism spectroscopy	670:700	Circular dichroism spectroscopy	670:700	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	3	50	theme	surface	645:651	arg1	density					661:667	the surface charges density	641:667	the surface charges density	641:667	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	5	51	theme	charge	968:973	arg1	property					975:982	Aβ charge property	965:982	Aβ charge property	965:982	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	4	52	theme	atomic	706:711	arg1	microscopy					719:728	atomic force microscopy	706:728	atomic force microscopy	706:728	Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms.
29549749	5	53	theme	different	794:802	arg1	values					807:812	different pH values	794:812	different pH values	794:812	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	5	54	theme	property	975:982	arg1	change					955:960	the change	951:960	the change of Aβ charge property	951:982	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	5	55	theme	CH	929:930	arg1	NPs					932:934	CH NPs	929:934	CH NPs	929:934	Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
29549749	1	56	theme	nanoparticles	230:242	arg1	factor					286:291	an important factor	273:291	an important factor influencing Aβ aggregation	273:318	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	1	56	theme	nanoparticles	230:242	arg1	charge					220:225	surface charge	212:225	surface charge of nanoparticles (NPs)	212:248	Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation.
29549749	0	57	theme	amyloid	86:92	arg1	aggregation					104:114	amyloid β-protein aggregation	86:114	amyloid β-protein aggregation	86:114	Charge effects of self-assembled chitosan-hyaluronic acid nanoparticles on inhibiting amyloid β-protein aggregation.
29549749	2	58	with	study	352:356	arg1	series					378:383	a series	376:383	a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV)	376:523	Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to -35 mV).
29549749	3	59	theme	Aβ	574:575	arg1	aggregation					577:587	Aβ aggregation	574:587	Aβ aggregation	574:587	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29549749	3	60	theme	positive	535:542	arg1	NPs					560:562	Both the positive and negative CH NPs	526:562	NPs	560:562	Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density.
29449636	0	0	theme	activities	94:103	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.	0:127	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	1	theme	dose-dependent	625:638	arg1	manner					640:645	a dose-dependent manner	623:645	a dose-dependent manner	623:645	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	0	2	theme	immunomodulation	77:92	arg1	activities					94:103	its immunomodulation activities	73:103	its immunomodulation activities on mouse B lymphocytes	73:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	0	3	from	elucidation	11:21	arg1	velutipes					59:67	Flammulina velutipes	48:67	Flammulina velutipes	48:67	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	0	3	from	elucidation	11:21	arg1	lymphocytes					116:126	mouse B lymphocytes	108:126	mouse B lymphocytes	108:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	5	4	theme	B	687:687	arg1	cells					689:693	B cells	687:693	B cells	687:693	The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment.
29449636	0	5	theme	mouse	108:112	arg1	lymphocytes					116:126	mouse B lymphocytes	108:126	mouse B lymphocytes	108:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	3	6	contain	containing	431:440	arg2	D-mannose					455:463	D-mannose	455:463	D-mannose	455:463	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg1	~ 1.50 × 104 Da					414:428	molecular weight ~ 1.50 × 104 Da	397:428	molecular weight ~ 1.50 × 104 Da	397:428	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg2	D-glucose					480:488	D-glucose	480:488	D-glucose	480:488	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg1	heteropolysaccharide					375:394	a homogeneous heteropolysaccharide	361:394	a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5	361:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg2	L-fucose					466:473	L-fucose	466:473	L-fucose	466:473	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg1	FVPB2					351:355	FVPB2	351:355	FVPB2	351:355	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	6	contain	containing	431:440	arg2	D-galactose					442:452	D-galactose	442:452	D-galactose	442:452	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	2	7	theme	FTIR	317:320	arg1	spectroscopy					322:333	FTIR spectroscopy	317:333	FTIR spectroscopy	317:333	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	2	7	theme	FTIR	317:320	arg1	analyses					292:299	monosaccharide composition and methylation analyses	249:299	monosaccharide composition and methylation analyses	249:299	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	0	8	from	velutipes	59:67	arg1	activities					94:103	its immunomodulation activities	73:103	its immunomodulation activities on mouse B lymphocytes	73:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	0	8	from	velutipes	59:67	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.	0:127	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	0	8	from	velutipes	59:67	arg1	polysaccharide					28:41	a polysaccharide	26:41	a polysaccharide from Flammulina velutipes	26:67	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	6	9	contain	has	738:740	arg2	potential					742:750	potential	742:750	potential	742:750	So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
29449636	6	9	contain	has	738:740	arg1	FVPB2					732:736	So FVPB2	729:736	So FVPB2	729:736	So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
29449636	3	10	theme	1.9:1.2:1:2.5	508:520	arg1	ratio					499:503	molar ratio	493:503	molar ratio of 1.9:1.2:1:2.5	493:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	5	11	theme	IgM	662:664	arg1	levels					652:657	The levels	648:657	The levels	648:657	The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment.
29449636	1	12	theme	fruiting	176:183	arg1	bodies					185:190	fruiting bodies	176:190	fruiting bodies of Flammulina velutipes	176:214	A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes.
29449636	0	13	theme	B	114:114	arg1	lymphocytes					116:126	mouse B lymphocytes	108:126	mouse B lymphocytes	108:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	14	theme	In	523:524	arg1	studies					549:555	In vitro immunomodulatory studies	523:555	In vitro immunomodulatory studies	523:555	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	5	15	theme	IgG	670:672	arg1	levels					652:657	The levels	648:657	The levels	648:657	The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment.
29449636	0	16	from	polysaccharide	28:41	arg1	lymphocytes					116:126	mouse B lymphocytes	108:126	mouse B lymphocytes	108:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	17	dep	In	523:524	arg1	vitro					526:530	vitro	526:530	vitro	526:530	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	0	18	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.	0:127	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	19	theme	spleen	601:606	arg1	lymphocytes					608:618	mouse spleen lymphocytes	595:618	mouse spleen lymphocytes	595:618	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	1	20	theme	Flammulina	195:204	arg1	velutipes					206:214	Flammulina velutipes	195:214	Flammulina velutipes	195:214	A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes.
29449636	3	21	theme	molecular	397:405	arg1	~ 1.50 × 104 Da					414:428	molecular weight ~ 1.50 × 104 Da	397:428	molecular weight ~ 1.50 × 104 Da	397:428	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	21	theme	molecular	397:405	arg1	heteropolysaccharide					375:394	a homogeneous heteropolysaccharide	361:394	a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5	361:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	4	22	theme	mouse	595:599	arg1	lymphocytes					608:618	mouse spleen lymphocytes	595:618	mouse spleen lymphocytes	595:618	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	1	23	theme	velutipes	206:214	arg1	bodies					185:190	fruiting bodies	176:190	fruiting bodies of Flammulina velutipes	176:214	A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes.
29449636	3	24	theme	weight	407:412	arg1	~ 1.50 × 104 Da					414:428	molecular weight ~ 1.50 × 104 Da	397:428	molecular weight ~ 1.50 × 104 Da	397:428	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	24	theme	weight	407:412	arg1	heteropolysaccharide					375:394	a homogeneous heteropolysaccharide	361:394	a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5	361:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	0	25	from	activities	94:103	arg1	lymphocytes					116:126	mouse B lymphocytes	108:126	mouse B lymphocytes	108:126	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	26	theme	lymphocytes	608:618	arg1	proliferation					578:590	FVPB2 induced proliferation	564:590	FVPB2 induced proliferation of mouse spleen lymphocytes	564:618	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	2	27	theme	monosaccharide	249:262	arg1	composition					264:274	monosaccharide composition	249:274	monosaccharide composition	249:274	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	0	28	theme	polysaccharide	28:41	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.	0:127	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	4	29	theme	induced	570:576	arg1	proliferation					578:590	FVPB2 induced proliferation	564:590	FVPB2 induced proliferation of mouse spleen lymphocytes	564:618	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	3	30	theme	homogeneous	363:373	arg1	~ 1.50 × 104 Da					414:428	molecular weight ~ 1.50 × 104 Da	397:428	molecular weight ~ 1.50 × 104 Da	397:428	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	30	theme	homogeneous	363:373	arg1	FVPB2					351:355	FVPB2	351:355	FVPB2	351:355	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	3	30	theme	homogeneous	363:373	arg1	heteropolysaccharide					375:394	a homogeneous heteropolysaccharide	361:394	a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5	361:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	4	31	theme	FVPB2	564:568	arg1	proliferation					578:590	FVPB2 induced proliferation	564:590	FVPB2 induced proliferation of mouse spleen lymphocytes	564:618	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	6	32	theme	immunomodulatory	774:789	arg1	nutraceutical					791:803	a new important immunomodulatory nutraceutical	758:803	a new important immunomodulatory nutraceutical	758:803	So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
29449636	0	33	theme	Flammulina	48:57	arg1	velutipes					59:67	Flammulina velutipes	48:67	Flammulina velutipes	48:67	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	0	34	from	lymphocytes	116:126	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.	0:127	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
29449636	6	35	theme	important	764:772	arg1	nutraceutical					791:803	a new important immunomodulatory nutraceutical	758:803	a new important immunomodulatory nutraceutical	758:803	So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
29449636	5	36	theme	FVPB2	712:716	arg1	treatment					718:726	FVPB2 treatment	712:726	FVPB2 treatment	712:726	The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment.
29449636	6	37	theme	new	760:762	arg1	nutraceutical					791:803	a new important immunomodulatory nutraceutical	758:803	a new important immunomodulatory nutraceutical	758:803	So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
29449636	1	38	theme	novel	131:135	arg1	FVPB2					152:156	A novel polysaccharide FVPB2	129:156	A novel polysaccharide FVPB2	129:156	A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes.
29449636	2	39	theme	methylation	280:290	arg1	UV-Visible					302:311	UV-Visible	302:311	UV-Visible	302:311	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	2	39	theme	methylation	280:290	arg1	spectroscopy					322:333	FTIR spectroscopy	317:333	FTIR spectroscopy	317:333	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	2	39	theme	methylation	280:290	arg1	analyses					292:299	monosaccharide composition and methylation analyses	249:299	monosaccharide composition and methylation analyses	249:299	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	1	40	theme	polysaccharide	137:150	arg1	FVPB2					152:156	A novel polysaccharide FVPB2	129:156	A novel polysaccharide FVPB2	129:156	A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes.
29449636	3	41	theme	molar	493:497	arg1	ratio					499:503	molar ratio	493:503	molar ratio of 1.9:1.2:1:2.5	493:520	FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5.
29449636	4	42	theme	immunomodulatory	532:547	arg1	studies					549:555	In vitro immunomodulatory studies	523:555	In vitro immunomodulatory studies	523:555	In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner.
29449636	2	43	theme	composition	264:274	arg1	UV-Visible					302:311	UV-Visible	302:311	UV-Visible	302:311	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	2	43	theme	composition	264:274	arg1	spectroscopy					322:333	FTIR spectroscopy	317:333	FTIR spectroscopy	317:333	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
29449636	2	43	theme	composition	264:274	arg1	analyses					292:299	monosaccharide composition and methylation analyses	249:299	monosaccharide composition and methylation analyses	249:299	Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR.
31147052	0	0	theme	structure	74:82	arg1	film					110:113	nano structure cellulose-polypyrrole-ZnO film	69:113	nano structure cellulose-polypyrrole-ZnO film	69:113	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	4	1	theme	BC	504:505	arg1	surface					507:513	the BC surface	500:513	the BC surface	500:513	The nanosized PPy-ZnO (50-150 nm) composites covered the BC surface.
31147052	8	2	theme	load	1017:1020	arg1	growth					997:1002	the growth	993:1002	the growth of microbial load in chicken thigh	993:1037	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	10	3	theme	storage	1353:1359	arg1	time-storage/temperature					1361:1384	storage time-storage/temperature	1353:1384	storage time-storage/temperature	1353:1384	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	0	4	theme	nano	69:72	arg1	film					110:113	nano structure cellulose-polypyrrole-ZnO film	69:113	nano structure cellulose-polypyrrole-ZnO film	69:113	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	8	5	theme	microbial	1007:1015	arg1	load					1017:1020	microbial load	1007:1020	microbial load	1007:1020	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	7	6	theme	microbial	805:813	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	6	theme	microbial	805:813	arg1	bacteria					854:861	Mesophilic and psychrophilic bacteria	825:861	bacteria	854:861	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	6	7	theme	temperature	617:627	arg1	effects					581:587	The effects	577:587	The effects of storage time and storage temperature on the chicken thigh characteristics	577:664	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	9	8	theme	antimicrobial	1213:1225	arg1	activity					1227:1234	antimicrobial activity	1213:1234	antimicrobial activity	1213:1234	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	3	9	theme	electron	371:378	arg1	SEM					392:394	SEM	392:394	SEM	392:394	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	3	9	theme	electron	371:378	arg1	microscopy					380:389	Scanning electron microscopy	362:389	Scanning electron microscopy (SEM)	362:395	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	8	10	theme	chicken	1025:1031	arg1	thigh					1033:1037	chicken thigh	1025:1037	chicken thigh	1025:1037	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	9	11	theme	chicken	1166:1172	arg1	thigh					1174:1178	chicken thigh	1166:1178	chicken thigh	1166:1178	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	6	12	theme	storage	609:615	arg1	temperature					617:627	storage temperature	609:627	storage temperature	609:627	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	0	13	theme	cellulose-polypyrrole-ZnO	84:108	arg1	film					110:113	nano structure cellulose-polypyrrole-ZnO film	69:113	nano structure cellulose-polypyrrole-ZnO film	69:113	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	9	14	theme	BC-PPy-ZnO	1080:1089	arg1	film					1091:1094	The BC-PPy-ZnO film	1076:1094	The BC-PPy-ZnO film	1076:1094	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	9	15	theme	thigh	1174:1178	arg1	properties					1152:1161	rheological properties	1140:1161	rheological properties of chicken thigh	1140:1178	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	7	16	theme	psychrophilic	840:852	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	16	theme	psychrophilic	840:852	arg1	bacteria					854:861	Mesophilic and psychrophilic bacteria	825:861	bacteria	854:861	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	10	17	theme	intelligent	1483:1493	arg1	packaging					1495:1503	intelligent packaging	1483:1503	intelligent packaging	1483:1503	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	10	18	dep	time	1434:1437	arg1	the					1422:1424	the	1422:1424	the	1422:1424	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	1	19	theme	bacterial	167:175	arg1	BC					188:189	BC	188:189	BC	188:189	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	1	19	theme	bacterial	167:175	arg1	cellulose					177:185	bacterial cellulose	167:185	bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO)	167:252	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	3	20	theme	Scanning	362:369	arg1	SEM					392:394	SEM	392:394	SEM	392:394	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	3	20	theme	Scanning	362:369	arg1	microscopy					380:389	Scanning electron microscopy	362:389	Scanning electron microscopy (SEM)	362:395	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	5	21	theme	thigh	570:574	arg1	packaging					549:557	packaging	549:557	packaging of chicken thigh	549:574	The BC-PPy-ZnO film was used for packaging of chicken thigh.
31147052	2	22	theme	Chemical	269:276	arg1	polymerization					278:291	Chemical polymerization	269:291	Chemical polymerization in the atmospheric condition	269:320	Chemical polymerization in the atmospheric condition was used for providing BC-PPy-ZnO film.
31147052	6	23	theme	composite	698:706	arg1	CCD					716:718	CCD	716:718	CCD	716:718	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	6	23	theme	composite	698:706	arg1	design					708:713	a central composite design	688:713	a central composite design (CCD)	688:719	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	10	24	theme	good	1292:1295	arg1	relations					1297:1305	good relations	1292:1305	good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging	1292:1503	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	10	25	theme	thigh	1474:1478	arg1	temperature					1451:1461	storage temperature	1443:1461	storage temperature	1443:1461	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	10	25	theme	thigh	1474:1478	arg1	time					1434:1437	storage time	1426:1437	storage time	1426:1437	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	10	26	theme	storage	1426:1432	arg1	time					1434:1437	storage time	1426:1437	storage time	1426:1437	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	7	27	theme	Mesophilic	825:834	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	27	theme	Mesophilic	825:834	arg1	bacteria					854:861	Mesophilic and psychrophilic bacteria	825:861	bacteria	854:861	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	6	28	theme	central	690:696	arg1	CCD					716:718	CCD	716:718	CCD	716:718	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	6	28	theme	central	690:696	arg1	design					708:713	a central composite design	688:713	a central composite design (CCD)	688:719	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	3	29	theme	film	430:433	arg1	morphology					435:444	BC-PPy-ZnO film morphology	419:444	BC-PPy-ZnO film morphology	419:444	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	0	30	theme	Intelligent	0:10	arg1	packaging					23:31	Intelligent and active packaging	0:31	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.	0:114	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	10	31	theme	chicken	1466:1472	arg1	thigh					1474:1478	chicken thigh	1466:1478	chicken thigh	1466:1478	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	10	32	theme	film	1315:1318	arg1	change					1342:1347	film electrical resistance change	1315:1347	film electrical resistance change	1315:1347	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	6	33	theme	storage	592:598	arg1	time					600:603	storage time	592:603	storage time	592:603	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	10	34	theme	storage	1443:1449	arg1	temperature					1451:1461	storage temperature	1443:1461	storage temperature	1443:1461	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	8	35	theme	BC-PPy-ZnO	962:971	arg1	film					973:976	the BC-PPy-ZnO film	958:976	the BC-PPy-ZnO film	958:976	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	0	36	theme	active	16:21	arg1	packaging					23:31	Intelligent and active packaging	0:31	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.	0:114	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	10	37	theme	resistance	1331:1340	arg1	change					1342:1347	film electrical resistance change	1315:1347	film electrical resistance change	1315:1347	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	4	38	theme	PPy-ZnO	461:467	arg1	composites					481:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites covered the BC surface.
31147052	2	39	theme	atmospheric	300:310	arg1	condition					312:320	the atmospheric condition	296:320	the atmospheric condition	296:320	Chemical polymerization in the atmospheric condition was used for providing BC-PPy-ZnO film.
31147052	0	40	theme	chicken	36:42	arg1	meat					50:53	chicken thigh meat	36:53	chicken thigh meat	36:53	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	4	41	theme	nanosized	451:459	arg1	composites					481:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites covered the BC surface.
31147052	6	42	theme	chicken	636:642	arg1	characteristics					650:664	the chicken thigh characteristics	632:664	the chicken thigh characteristics	632:664	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	3	43	theme	BC-PPy-ZnO	419:428	arg1	morphology					435:444	BC-PPy-ZnO film morphology	419:444	BC-PPy-ZnO film morphology	419:444	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	7	44	theme	chicken	760:766	arg1	antioxidant					881:891	antioxidant	881:891	antioxidant	881:891	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	44	theme	chicken	760:766	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	44	theme	chicken	760:766	arg1	characteristics					774:788	the chicken thigh characteristics	756:788	the chicken thigh characteristics	756:788	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	44	theme	chicken	760:766	arg1	properties					909:918	rheological properties	897:918	rheological properties	897:918	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	44	theme	chicken	760:766	arg1	property					871:878	color property	865:878	color property	865:878	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	44	theme	chicken	760:766	arg1	pH					801:802	pH	801:802	pH	801:802	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	8	45	theme	increasing	1064:1073	arg1	pH					1061:1062	the pH increasing	1057:1073	the pH increasing	1057:1073	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	4	46	theme	50-150 nm	470:478	arg1	composites					481:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites	447:490	The nanosized PPy-ZnO (50-150 nm) composites covered the BC surface.
31147052	1	47	theme	polypyrrole-Zinc	204:219	arg1	BC-PPy-ZnO					242:251	BC-PPy-ZnO	242:251	BC-PPy-ZnO	242:251	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	1	47	theme	polypyrrole-Zinc	204:219	arg1	nanocomposite					227:239	polypyrrole-Zinc oxide nanocomposite	204:239	polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO)	204:252	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	0	48	theme	meat	50:53	arg1	packaging					23:31	Intelligent and active packaging	0:31	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.	0:114	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	5	49	used	used	540:543	arg2	film					531:534	The BC-PPy-ZnO film	516:534	The BC-PPy-ZnO film	516:534	The BC-PPy-ZnO film was used for packaging of chicken thigh.
31147052	7	50	from	effects	726:732	arg1	antioxidant					881:891	antioxidant	881:891	antioxidant	881:891	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	50	from	effects	726:732	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	50	from	effects	726:732	arg1	characteristics					774:788	the chicken thigh characteristics	756:788	the chicken thigh characteristics	756:788	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	50	from	effects	726:732	arg1	properties					909:918	rheological properties	897:918	rheological properties	897:918	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	50	from	effects	726:732	arg1	property					871:878	color property	865:878	color property	865:878	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	50	from	effects	726:732	arg1	pH					801:802	pH	801:802	pH	801:802	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	9	51	theme	active	1239:1244	arg1	packaging					1246:1254	active packaging	1239:1254	active packaging	1239:1254	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	0	52	theme	thigh	44:48	arg1	meat					50:53	chicken thigh meat	36:53	chicken thigh meat	36:53	Intelligent and active packaging of chicken thigh meat by conducting nano structure cellulose-polypyrrole-ZnO film.
31147052	3	53	used	used	401:404	arg2	microscopy					380:389	Scanning electron microscopy	362:389	Scanning electron microscopy (SEM)	362:395	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	3	53	used	used	401:404	arg2	SEM					392:394	SEM	392:394	SEM	392:394	Scanning electron microscopy (SEM) was used for studying BC-PPy-ZnO film morphology.
31147052	9	54	theme	shelf	1115:1119	arg1	life					1121:1124	the shelf life	1111:1124	the shelf life	1111:1124	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	2	55	theme	BC-PPy-ZnO	345:354	arg1	film					356:359	BC-PPy-ZnO film	345:359	BC-PPy-ZnO film	345:359	Chemical polymerization in the atmospheric condition was used for providing BC-PPy-ZnO film.
31147052	2	56	from	polymerization	278:291	arg1	condition					312:320	the atmospheric condition	296:320	the atmospheric condition	296:320	Chemical polymerization in the atmospheric condition was used for providing BC-PPy-ZnO film.
31147052	5	57	theme	chicken	562:568	arg1	thigh					570:574	chicken thigh	562:574	chicken thigh	562:574	The BC-PPy-ZnO film was used for packaging of chicken thigh.
31147052	1	58	theme	intelligent	129:139	arg1	films					152:156	intelligent and active films	129:156	intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO)	129:252	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	7	59	theme	rheological	897:907	arg1	properties					909:918	rheological properties	897:918	rheological properties	897:918	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	1	60	theme	oxide	221:225	arg1	BC-PPy-ZnO					242:251	BC-PPy-ZnO	242:251	BC-PPy-ZnO	242:251	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	1	60	theme	oxide	221:225	arg1	nanocomposite					227:239	polypyrrole-Zinc oxide nanocomposite	204:239	polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO)	204:252	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	10	61	theme	electrical	1320:1329	arg1	change					1342:1347	film electrical resistance change	1315:1347	film electrical resistance change	1315:1347	According to the results there are good relations between film electrical resistance change and storage time-storage/temperature that this could help us to estimate the storage time and storage temperature of chicken thigh as intelligent packaging.
31147052	6	62	from	effects	581:587	arg1	characteristics					650:664	the chicken thigh characteristics	632:664	the chicken thigh characteristics	632:664	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	5	63	theme	BC-PPy-ZnO	520:529	arg1	film					531:534	The BC-PPy-ZnO film	516:534	The BC-PPy-ZnO film	516:534	The BC-PPy-ZnO film was used for packaging of chicken thigh.
31147052	8	64	from	growth	997:1002	arg1	thigh					1033:1037	chicken thigh	1025:1037	chicken thigh	1025:1037	The results showed that the BC-PPy-ZnO film could decrease the growth of microbial load in chicken thigh and could control the pH increasing.
31147052	6	65	theme	thigh	644:648	arg1	characteristics					650:664	the chicken thigh characteristics	632:664	the chicken thigh characteristics	632:664	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	7	66	theme	film	748:751	arg1	effects					726:732	The effects	722:732	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties	722:918	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	6	67	theme	time	600:603	arg1	effects					581:587	The effects	577:587	The effects of storage time and storage temperature on the chicken thigh characteristics	577:664	The effects of storage time and storage temperature on the chicken thigh characteristics were studied based on a central composite design (CCD).
31147052	1	68	theme	active	145:150	arg1	films					152:156	intelligent and active films	129:156	intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO)	129:252	In this work intelligent and active films based on bacterial cellulose (BC) modified by polypyrrole-Zinc oxide nanocomposite (BC-PPy-ZnO) were provided.
31147052	2	69	used	used	326:329	arg2	polymerization					278:291	Chemical polymerization	269:291	Chemical polymerization in the atmospheric condition	269:320	Chemical polymerization in the atmospheric condition was used for providing BC-PPy-ZnO film.
31147052	9	70	theme	rheological	1140:1150	arg1	properties					1152:1161	rheological properties	1140:1161	rheological properties of chicken thigh	1140:1178	The BC-PPy-ZnO film could increase the shelf life and stabilize rheological properties of chicken thigh by increasing of antioxidant and antimicrobial activity as active packaging.
31147052	7	71	theme	BC-PPy-ZnO	737:746	arg1	film					748:751	BC-PPy-ZnO film	737:751	BC-PPy-ZnO film	737:751	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	72	theme	color	865:869	arg1	property					871:878	color property	865:878	color property	865:878	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	antioxidant					881:891	antioxidant	881:891	antioxidant	881:891	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	property					815:822	microbial property	805:822	microbial property (Mesophilic and psychrophilic bacteria)	805:862	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	characteristics					774:788	the chicken thigh characteristics	756:788	the chicken thigh characteristics	756:788	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	properties					909:918	rheological properties	897:918	rheological properties	897:918	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	property					871:878	color property	865:878	color property	865:878	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
31147052	7	73	theme	thigh	768:772	arg1	pH					801:802	pH	801:802	pH	801:802	The effects of BC-PPy-ZnO film on the chicken thigh characteristics, including pH, microbial property (Mesophilic and psychrophilic bacteria), color property, antioxidant and rheological properties were studied.
30275395	6	0	theme	ursolic	987:993	arg1	acid					995:998	ursolic acid	987:998	ursolic acid	987:998	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	8	1	theme	α-glucosidase	1433:1445	arg1	activity					1458:1465	α-glucosidase inhibitory activity	1433:1465	α-glucosidase inhibitory activity	1433:1465	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	2	2	theme	dried	343:347	arg1	fruits					349:354	the dried fruits	339:354	the dried fruits of C. speciosa	339:369	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	4	3	theme	production	812:821	arg1	areas					823:827	other production areas	806:827	other production areas	806:827	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	3	4	theme	speciosa	482:489	arg1	fruits					469:474	dried fruits	463:474	dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	463:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	9	5	theme	correlation	1481:1491	arg1	analysis					1493:1500	correlation analysis	1481:1500	correlation analysis	1481:1500	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	7	6	theme	high	1262:1265	arg1	levels					1267:1272	high levels	1262:1272	high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g)	1262:1345	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	9	7	theme	α-glucosidase	1738:1750	arg1	activity					1763:1770	α-glucosidase inhibitory activity	1738:1770	α-glucosidase inhibitory activity of the plant	1738:1783	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	1	8	theme	medicinal	182:190	arg1	Nakai					171:175	Nakai	171:175	Nakai	171:175	Chaenomeles speciosa (Sweet) Nakai is a medicinal plant.
30275395	1	8	theme	medicinal	182:190	arg1	plant					192:196	a medicinal plant	180:196	a medicinal plant	180:196	Chaenomeles speciosa (Sweet) Nakai is a medicinal plant.
30275395	9	9	theme	total	1516:1520	arg1	components					1598:1607	the major components	1588:1607	the major components responsible for the antioxidant activity of C. speciosa	1588:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	9	theme	total	1516:1520	arg1	polysaccharide					1551:1564	polysaccharide	1551:1564	polysaccharide	1551:1564	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	9	theme	total	1516:1520	arg1	flavonoid					1522:1530	total flavonoid	1516:1530	total flavonoid	1516:1530	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	9	theme	total	1516:1520	arg1	polyphenol					1539:1548	total polyphenol	1533:1548	total polyphenol	1533:1548	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	9	theme	total	1516:1520	arg1	acid					1578:1581	ursolic acid	1570:1581	ursolic acid	1570:1581	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	6	10	theme	±	1085:1085	arg1	μg/g					1093:1096	321.06 ± 14.64 μg/g	1078:1096	321.06 ± 14.64 μg/g	1078:1096	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	6	10	theme	±	1085:1085	arg1	Anhui					1071:1075	Anhui	1071:1075	Anhui (321.06 ± 14.64 μg/g)	1071:1097	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	7	11	theme	ursolic	1236:1242	arg1	acid					1244:1247	ursolic acid	1236:1247	ursolic acid	1236:1247	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	2	12	theme	antioxidant	277:287	arg1	profiles					267:274	the chemical profiles	254:274	the chemical profiles	254:274	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	12	theme	antioxidant	277:287	arg1	activity					289:296	antioxidant activity	277:296	antioxidant activity	277:296	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	7	13	dep	acid	1211:1214	arg1	contents					1249:1256	contents	1249:1256	contents	1249:1256	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	14	theme	amino	1205:1209	arg1	acid					1211:1214	free amino acid	1200:1214	free amino acid	1200:1214	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	3	15	from	provinces	534:542	arg1	components					449:458	the chemical components	436:458	the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	436:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	3	15	from	provinces	534:542	arg1	fruits					469:474	dried fruits	463:474	dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	463:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	3	15	from	provinces	534:542	arg1	China					547:551	China	547:551	China	547:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	10	16	theme	speciosa	1850:1857	arg1	quality					1836:1842	the quality	1832:1842	the quality of C. speciosa in the different production areas	1832:1891	These results would be helpful for evaluating the quality of C. speciosa in the different production areas.
30275395	3	17	theme	α-glucosidase	597:609	arg1	activity					622:629	α-glucosidase inhibitory activity	597:629	α-glucosidase inhibitory activity	597:629	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	1	18	theme	Chaenomeles	142:152	arg1	Sweet					164:168	Sweet	164:168	Sweet	164:168	Chaenomeles speciosa (Sweet) Nakai is a medicinal plant.
30275395	1	18	theme	Chaenomeles	142:152	arg1	speciosa					154:161	Chaenomeles speciosa	142:161	Chaenomeles speciosa (Sweet) Nakai	142:175	Chaenomeles speciosa (Sweet) Nakai is a medicinal plant.
30275395	9	19	theme	ursolic	1570:1576	arg1	components					1598:1607	the major components	1588:1607	the major components responsible for the antioxidant activity of C. speciosa	1588:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	19	theme	ursolic	1570:1576	arg1	polysaccharide					1551:1564	polysaccharide	1551:1564	polysaccharide	1551:1564	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	19	theme	ursolic	1570:1576	arg1	acid					1578:1581	ursolic acid	1570:1581	ursolic acid	1570:1581	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	19	theme	ursolic	1570:1576	arg1	polyphenol					1539:1548	total polyphenol	1533:1548	total polyphenol	1533:1548	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	19	theme	ursolic	1570:1576	arg1	flavonoid					1522:1530	total flavonoid	1516:1530	total flavonoid	1516:1530	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	4	20	theme	total	667:671	arg1	flavonoid					673:681	higher total flavonoid	660:681	higher total flavonoid (47.92 ± 3.79 mg/g)	660:701	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	20	theme	total	667:671	arg1	mg/g					697:700	47.92 ± 3.79 mg/g	684:700	47.92 ± 3.79 mg/g	684:700	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	10	21	theme	production	1876:1885	arg1	areas					1887:1891	the different production areas	1862:1891	the different production areas	1862:1891	These results would be helpful for evaluating the quality of C. speciosa in the different production areas.
30275395	10	22	from	quality	1836:1842	arg1	areas					1887:1891	the different production areas	1862:1891	the different production areas	1862:1891	These results would be helpful for evaluating the quality of C. speciosa in the different production areas.
30275395	9	23	theme	major	1592:1596	arg1	components					1598:1607	the major components	1588:1607	the major components responsible for the antioxidant activity of C. speciosa	1588:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	23	theme	major	1592:1596	arg1	polysaccharide					1551:1564	polysaccharide	1551:1564	polysaccharide	1551:1564	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	23	theme	major	1592:1596	arg1	acid					1578:1581	ursolic acid	1570:1581	ursolic acid	1570:1581	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	23	theme	major	1592:1596	arg1	polyphenol					1539:1548	total polyphenol	1533:1548	total polyphenol	1533:1548	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	23	theme	major	1592:1596	arg1	flavonoid					1522:1530	total flavonoid	1516:1530	total flavonoid	1516:1530	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	4	24	theme	29.15	722:726	arg1	±					728:728	±	728:728	±	728:728	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	8	25	dep	activity	1420:1427	arg1	the					1396:1398	the	1396:1398	the	1396:1398	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	4	26	dep	flavonoid	673:681	arg1	contents					780:787	contents	780:787	contents	780:787	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	9	27	theme	responsible	1609:1619	arg1	components					1598:1607	the major components	1588:1607	the major components responsible for the antioxidant activity of C. speciosa	1588:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	27	theme	responsible	1609:1619	arg1	polysaccharide					1551:1564	polysaccharide	1551:1564	polysaccharide	1551:1564	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	27	theme	responsible	1609:1619	arg1	acid					1578:1581	ursolic acid	1570:1581	ursolic acid	1570:1581	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	27	theme	responsible	1609:1619	arg1	polyphenol					1539:1548	total polyphenol	1533:1548	total polyphenol	1533:1548	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	27	theme	responsible	1609:1619	arg1	flavonoid					1522:1530	total flavonoid	1516:1530	total flavonoid	1516:1530	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	4	28	theme	0.29	730:733	arg1	±					728:728	±	728:728	±	728:728	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	2	29	theme	production	386:395	arg1	regions					397:403	different production regions	376:403	different production regions	376:403	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	0	30	theme	Production	115:124	arg1	Areas					126:130	Four Production Areas	110:130	Four Production Areas in China	110:139	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	7	31	contain	had	1141:1143	arg2	flavonoid					1155:1163	low total flavonoid	1145:1163	low total flavonoid	1145:1163	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	31	contain	had	1141:1143	arg2	polyphenol					1172:1181	total polyphenol	1166:1181	total polyphenol	1166:1181	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	31	contain	had	1141:1143	arg2	acid					1211:1214	free amino acid	1200:1214	free amino acid	1200:1214	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	31	contain	had	1141:1143	arg2	polysaccharide					1184:1197	polysaccharide	1184:1197	polysaccharide	1184:1197	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	31	contain	had	1141:1143	arg1	speciosa					1117:1124	C. speciosa	1114:1124	C. speciosa from Chongqing	1114:1139	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	31	contain	had	1141:1143	arg2	levels					1267:1272	high levels	1262:1272	high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g)	1262:1345	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	5	32	from	Zhejiang	851:858	arg1	Samples					830:836	Samples	830:836	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg)	830:887	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	6	33	theme	Oleanolic	968:976	arg1	acid					978:981	Oleanolic acid	968:981	Oleanolic acid	968:981	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	0	34	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	3	35	theme	Chongqing	504:512	arg1	provinces					534:542	Yunnan, Chongqing, Zhejiang and Anhui provinces	496:542	Yunnan, Chongqing, Zhejiang and Anhui provinces in China	496:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	5	36	theme	>	875:875	arg1	mg/kg					882:886	all > 3200 mg/kg	871:886	all > 3200 mg/kg	871:886	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	5	36	theme	>	875:875	arg1	Anhui					864:868	Anhui	864:868	Anhui	864:868	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	4	37	theme	27.60	761:765	arg1	±					767:767	±	767:767	±	767:767	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	7	38	theme	total	1149:1153	arg1	flavonoid					1155:1163	low total flavonoid	1145:1163	low total flavonoid	1145:1163	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	4	39	theme	1.56	769:772	arg1	±					767:767	±	767:767	±	767:767	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	0	40	theme	α-Glucosidase	47:59	arg1	Activity					72:79	α-Glucosidase Inhibitory Activity	47:79	α-Glucosidase Inhibitory Activity	47:79	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	9	41	theme	total	1672:1676	arg1	polysaccharide					1692:1705	polysaccharide	1692:1705	polysaccharide	1692:1705	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	41	theme	total	1672:1676	arg1	flavonoid					1678:1686	total flavonoid	1672:1686	total flavonoid	1672:1686	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	41	theme	total	1672:1676	arg1	contributors					1721:1732	the main contributors	1712:1732	the main contributors for α-glucosidase inhibitory activity of the plant	1712:1783	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	4	42	contain	had	656:658	arg2	flavonoid					673:681	higher total flavonoid	660:681	higher total flavonoid (47.92 ± 3.79 mg/g)	660:701	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg2	polyphenol					710:719	total polyphenol	704:719	total polyphenol (29.15 ± 0.29 mg/g)	704:739	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg2	mg/g					697:700	47.92 ± 3.79 mg/g	684:700	47.92 ± 3.79 mg/g	684:700	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg2	mg/g					735:738	29.15 ± 0.29 mg/g	722:738	29.15 ± 0.29 mg/g	722:738	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg2	polysaccharide					745:758	polysaccharide	745:758	polysaccharide (27.60 ± 1.56 mg/g)	745:778	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg1	speciosa					635:642	C. speciosa	632:642	C. speciosa from Yunnan	632:654	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	42	contain	had	656:658	arg2	mg/g					774:777	27.60 ± 1.56 mg/g	761:777	27.60 ± 1.56 mg/g	761:777	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	3	43	theme	dried	463:467	arg1	fruits					469:474	dried fruits	463:474	dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	463:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	7	44	theme	stearic	1315:1321	arg1	mg/g					1341:1344	2.23 ± 0.08 mg/g	1329:1344	2.23 ± 0.08 mg/g	1329:1344	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	44	theme	stearic	1315:1321	arg1	acid					1323:1326	stearic acid	1315:1326	stearic acid (2.23 ± 0.08 mg/g)	1315:1345	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	5	45	theme	free	900:903	arg1	contents					916:923	higher free amino acid contents	893:923	higher free amino acid contents	893:923	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	5	46	theme	acid	911:914	arg1	contents					916:923	higher free amino acid contents	893:923	higher free amino acid contents	893:923	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	6	47	theme	acid	995:998	arg1	levels					1000:1005	Oleanolic acid and ursolic acid levels	968:1005	Oleanolic acid and ursolic acid levels	968:1005	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	8	48	theme	antioxidant	1408:1418	arg1	activity					1420:1427	antioxidant activity	1408:1427	antioxidant activity	1408:1427	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	2	49	theme	fruits	349:354	arg1	profiles					267:274	the chemical profiles	254:274	the chemical profiles	254:274	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	49	theme	fruits	349:354	arg1	activity					289:296	antioxidant activity	277:296	antioxidant activity	277:296	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	49	theme	fruits	349:354	arg1	activity					327:334	α-glucosidase inhibitory activity	302:334	α-glucosidase inhibitory activity	302:334	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	9	50	theme	main	1716:1719	arg1	polysaccharide					1692:1705	polysaccharide	1692:1705	polysaccharide	1692:1705	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	50	theme	main	1716:1719	arg1	flavonoid					1678:1686	total flavonoid	1672:1686	total flavonoid	1672:1686	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	50	theme	main	1716:1719	arg1	contributors					1721:1732	the main contributors	1712:1732	the main contributors for α-glucosidase inhibitory activity of the plant	1712:1783	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	3	51	theme	Zhejiang	515:522	arg1	provinces					534:542	Yunnan, Chongqing, Zhejiang and Anhui provinces	496:542	Yunnan, Chongqing, Zhejiang and Anhui provinces in China	496:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	4	52	theme	other	806:810	arg1	areas					823:827	other production areas	806:827	other production areas	806:827	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	7	53	theme	palmitic	1277:1284	arg1	mg/g					1305:1308	12.04 ± 0.02 mg/g	1292:1308	12.04 ± 0.02 mg/g	1292:1308	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	7	53	theme	palmitic	1277:1284	arg1	acid					1286:1289	palmitic acid	1277:1289	palmitic acid (12.04 ± 0.02 mg/g)	1277:1309	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	5	54	contain	had	889:891	arg1	Samples					830:836	Samples	830:836	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg)	830:887	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	5	54	contain	had	889:891	arg2	contents					916:923	higher free amino acid contents	893:923	higher free amino acid contents	893:923	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	8	55	theme	inhibitory	1447:1456	arg1	activity					1458:1465	α-glucosidase inhibitory activity	1433:1465	α-glucosidase inhibitory activity	1433:1465	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	2	56	theme	chemical	258:265	arg1	profiles					267:274	the chemical profiles	254:274	the chemical profiles	254:274	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	56	theme	chemical	258:265	arg1	activity					289:296	antioxidant activity	277:296	antioxidant activity	277:296	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	56	theme	chemical	258:265	arg1	activity					327:334	α-glucosidase inhibitory activity	302:334	α-glucosidase inhibitory activity	302:334	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	7	57	theme	acid	1286:1289	arg1	levels					1267:1272	high levels	1262:1272	high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g)	1262:1345	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	6	58	theme	±	1053:1053	arg1	μg/g					1061:1064	555.98 ± 20.88 μg/g	1046:1064	555.98 ± 20.88 μg/g	1046:1064	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	6	58	theme	±	1053:1053	arg1	Zhejiang					1036:1043	Zhejiang	1036:1043	Zhejiang (555.98 ± 20.88 μg/g)	1036:1065	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	9	59	theme	inhibitory	1752:1761	arg1	activity					1763:1770	α-glucosidase inhibitory activity	1738:1770	α-glucosidase inhibitory activity of the plant	1738:1783	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	2	60	theme	α-glucosidase	302:314	arg1	profiles					267:274	the chemical profiles	254:274	the chemical profiles	254:274	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	60	theme	α-glucosidase	302:314	arg1	activity					327:334	α-glucosidase inhibitory activity	302:334	α-glucosidase inhibitory activity	302:334	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	7	61	from	Chongqing	1131:1139	arg1	speciosa					1117:1124	C. speciosa	1114:1124	C. speciosa from Chongqing	1114:1139	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	3	62	theme	Anhui	528:532	arg1	provinces					534:542	Yunnan, Chongqing, Zhejiang and Anhui provinces	496:542	Yunnan, Chongqing, Zhejiang and Anhui provinces in China	496:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	9	63	theme	plant	1779:1783	arg1	activity					1763:1770	α-glucosidase inhibitory activity	1738:1770	α-glucosidase inhibitory activity of the plant	1738:1783	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	64	theme	total	1533:1537	arg1	components					1598:1607	the major components	1588:1607	the major components responsible for the antioxidant activity of C. speciosa	1588:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	64	theme	total	1533:1537	arg1	polysaccharide					1551:1564	polysaccharide	1551:1564	polysaccharide	1551:1564	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	64	theme	total	1533:1537	arg1	acid					1578:1581	ursolic acid	1570:1581	ursolic acid	1570:1581	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	64	theme	total	1533:1537	arg1	polyphenol					1539:1548	total polyphenol	1533:1548	total polyphenol	1533:1548	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	9	64	theme	total	1533:1537	arg1	flavonoid					1522:1530	total flavonoid	1516:1530	total flavonoid	1516:1530	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	0	65	from	Areas	126:130	arg1	Activity					72:79	α-Glucosidase Inhibitory Activity	47:79	α-Glucosidase Inhibitory Activity	47:79	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	0	65	from	Areas	126:130	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	0	65	from	Areas	126:130	arg1	Activity					34:41	Antioxidant Activity	22:41	Antioxidant Activity	22:41	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	0	65	from	Areas	126:130	arg1	China					135:139	China	135:139	China	135:139	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	7	66	theme	oleanolic	1217:1225	arg1	acid					1227:1230	oleanolic acid	1217:1230	oleanolic acid	1217:1230	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	4	67	theme	47.92	684:688	arg1	±					690:690	±	690:690	±	690:690	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	68	theme	3.79	692:695	arg1	±					690:690	±	690:690	±	690:690	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	69	from	areas	823:827	arg1	plants					794:799	plants	794:799	plants from other production areas	794:827	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	3	70	theme	antioxidant	572:582	arg1	activity					584:591	the antioxidant activity	568:591	the antioxidant activity	568:591	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	3	71	theme	fruits	469:474	arg1	components					449:458	the chemical components	436:458	the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	436:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	7	72	theme	free	1200:1203	arg1	acid					1211:1214	free amino acid	1200:1214	free amino acid	1200:1214	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	4	73	theme	higher	660:665	arg1	flavonoid					673:681	higher total flavonoid	660:681	higher total flavonoid (47.92 ± 3.79 mg/g)	660:701	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	73	theme	higher	660:665	arg1	mg/g					697:700	47.92 ± 3.79 mg/g	684:700	47.92 ± 3.79 mg/g	684:700	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	6	74	from	Anhui	1071:1075	arg1	samples					1023:1029	samples	1023:1029	samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively	1023:1111	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	0	75	theme	Speciosa	96:103	arg1	Activity					72:79	α-Glucosidase Inhibitory Activity	47:79	α-Glucosidase Inhibitory Activity	47:79	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	0	75	theme	Speciosa	96:103	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	0	75	theme	Speciosa	96:103	arg1	Activity					34:41	Antioxidant Activity	22:41	Antioxidant Activity	22:41	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	10	76	theme	different	1866:1874	arg1	areas					1887:1891	the different production areas	1862:1891	the different production areas	1862:1891	These results would be helpful for evaluating the quality of C. speciosa in the different production areas.
30275395	2	77	theme	inhibitory	316:325	arg1	profiles					267:274	the chemical profiles	254:274	the chemical profiles	254:274	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	2	77	theme	inhibitory	316:325	arg1	activity					327:334	α-glucosidase inhibitory activity	302:334	α-glucosidase inhibitory activity	302:334	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	6	78	theme	acid	978:981	arg1	levels					1000:1005	Oleanolic acid and ursolic acid levels	968:1005	Oleanolic acid and ursolic acid levels	968:1005	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	2	79	theme	different	376:384	arg1	regions					397:403	different production regions	376:403	different production regions	376:403	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	7	80	theme	total	1166:1170	arg1	polyphenol					1172:1181	total polyphenol	1166:1181	total polyphenol	1166:1181	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	9	81	theme	antioxidant	1629:1639	arg1	activity					1641:1648	the antioxidant activity	1625:1648	the antioxidant activity of C. speciosa	1625:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	6	82	from	Zhejiang	1036:1043	arg1	samples					1023:1029	samples	1023:1029	samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively	1023:1111	Oleanolic acid and ursolic acid levels were highest in samples from Zhejiang (555.98 ± 20.88 μg/g) and Anhui (321.06 ± 14.64 μg/g), respectively.
30275395	2	83	theme	speciosa	362:369	arg1	fruits					349:354	the dried fruits	339:354	the dried fruits of C. speciosa	339:369	Until date, there are no studies focusing on comparing the chemical profiles, antioxidant activity and α-glucosidase inhibitory activity of the dried fruits of C. speciosa from different production regions.
30275395	0	84	theme	Antioxidant	22:32	arg1	Activity					34:41	Antioxidant Activity	22:41	Antioxidant Activity	22:41	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	8	85	theme	production	1359:1368	arg1	areas					1370:1374	four production areas	1354:1374	four production areas	1354:1374	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	4	86	theme	total	704:708	arg1	mg/g					735:738	29.15 ± 0.29 mg/g	722:738	29.15 ± 0.29 mg/g	722:738	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	4	86	theme	total	704:708	arg1	polyphenol					710:719	total polyphenol	704:719	total polyphenol (29.15 ± 0.29 mg/g)	704:739	C. speciosa from Yunnan had higher total flavonoid (47.92 ± 3.79 mg/g), total polyphenol (29.15 ± 0.29 mg/g) and polysaccharide (27.60 ± 1.56 mg/g) contents than plants from other production areas.
30275395	7	87	theme	low	1145:1147	arg1	flavonoid					1155:1163	low total flavonoid	1145:1163	low total flavonoid	1145:1163	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	9	88	theme	speciosa	1656:1663	arg1	activity					1641:1648	the antioxidant activity	1625:1648	the antioxidant activity of C. speciosa	1625:1663	In addition, correlation analysis revealed that total flavonoid, total polyphenol, polysaccharide and ursolic acid were the major components responsible for the antioxidant activity of C. speciosa, while total flavonoid and polysaccharide were the main contributors for α-glucosidase inhibitory activity of the plant.
30275395	3	89	theme	inhibitory	611:620	arg1	activity					622:629	α-glucosidase inhibitory activity	597:629	α-glucosidase inhibitory activity	597:629	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	3	90	theme	chemical	440:447	arg1	components					449:458	the chemical components	436:458	the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China	436:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	0	91	theme	Inhibitory	61:70	arg1	Activity					72:79	α-Glucosidase Inhibitory Activity	47:79	α-Glucosidase Inhibitory Activity	47:79	Chemical Composition, Antioxidant Activity and α-Glucosidase Inhibitory Activity of Chaenomeles Speciosa from Four Production Areas in China.
30275395	3	92	from	components	449:458	arg1	provinces					534:542	Yunnan, Chongqing, Zhejiang and Anhui provinces	496:542	Yunnan, Chongqing, Zhejiang and Anhui provinces in China	496:551	In the study, we investigated the chemical components of dried fruits of C. speciosa from Yunnan, Chongqing, Zhejiang and Anhui provinces in China in relation to the antioxidant activity and α-glucosidase inhibitory activity.
30275395	5	93	theme	higher	893:898	arg1	contents					916:923	higher free amino acid contents	893:923	higher free amino acid contents	893:923	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	8	94	theme	highest	1400:1406	arg1	activity					1420:1427	antioxidant activity	1408:1427	antioxidant activity	1408:1427	Among four production areas, Yunnan represented the highest antioxidant activity and α-glucosidase inhibitory activity.
30275395	7	95	theme	C.	1114:1115	arg1	speciosa					1117:1124	C. speciosa	1114:1124	C. speciosa from Chongqing	1114:1139	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	5	96	from	Anhui	864:868	arg1	Samples					830:836	Samples	830:836	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg)	830:887	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
30275395	7	97	theme	acid	1323:1326	arg1	levels					1267:1272	high levels	1262:1272	high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g)	1262:1345	C. speciosa from Chongqing had low total flavonoid, total polyphenol, polysaccharide, free amino acid, oleanolic acid and ursolic acid contents but high levels of palmitic acid (12.04 ± 0.02 mg/g) and stearic acid (2.23 ± 0.08 mg/g).
30275395	5	98	theme	amino	905:909	arg1	contents					916:923	higher free amino acid contents	893:923	higher free amino acid contents	893:923	Samples from Yunnan, Zhejiang and Anhui (all > 3200 mg/kg) had higher free amino acid contents than those from Chongqing (2286.66 mg/kg).
29414153	2	0	theme	same	500:503	arg1	ratio					517:521	the same composition ratio	496:521	the same composition ratio	496:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	0	1	theme	corresponding	70:82	arg1	mixture					84:90	its corresponding mixture	66:90	its corresponding mixture of cellulose, xylan, and lignin	66:122	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	1	2	theme	above	348:352	arg1	components					354:363	the above components	344:363	the above components	344:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	5	3	theme	original	897:904	arg1	sample					914:919	the original biomass sample	893:919	the original biomass sample	893:919	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	1	4	theme	glucose	251:257	arg1	production					259:268	glucose production	251:268	glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	251:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	0	5	theme	cellulose	95:103	arg1	mixture					84:90	its corresponding mixture	66:90	its corresponding mixture of cellulose, xylan, and lignin	66:122	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	0	5	theme	cellulose	95:103	arg1	study					12:16	Comparative study	0:16	Comparative study of hydrothermal pretreatment for rice straw	0:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	5	6	theme	pretreatment	961:972	arg1	efficiency					934:943	the efficiency	930:943	the efficiency of hydrothermal pretreatment	930:972	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	2	7	contain	containing	451:460	arg2	compounds					483:491	the individual model compounds	462:491	the individual model compounds	462:491	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	2	7	contain	containing	451:460	arg1	mixture					443:449	a mixture	441:449	a mixture containing the individual model compounds in the same composition ratio	441:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	5	8	theme	cross-linking	847:859	arg1	presence					835:842	the presence	831:842	the presence of cross-linking between cellulose and lignin in the original biomass sample	831:919	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	0	9	theme	xylan	106:110	arg1	mixture					84:90	its corresponding mixture	66:90	its corresponding mixture of cellulose, xylan, and lignin	66:122	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	0	9	theme	xylan	106:110	arg1	study					12:16	Comparative study	0:16	Comparative study of hydrothermal pretreatment for rice straw	0:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	3	10	theme	straw	535:539	arg1	biomass					541:547	rice straw biomass	530:547	rice straw biomass	530:547	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	2	11	theme	hydrothermal	400:411	arg1	pretreatment					413:424	the hydrothermal pretreatment	396:424	the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio	396:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	2	12	theme	individual	466:475	arg1	compounds					483:491	the individual model compounds	462:491	the individual model compounds	462:491	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	5	13	theme	different	775:783	arg1	results					785:791	different results	775:791	different results	775:791	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	1	14	from	cellulose	275:283	arg1	production					259:268	glucose production	251:268	glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	251:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	1	15	theme	hemicellulose	162:174	arg1	effect					152:157	the effect	148:157	the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	148:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	0	16	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of hydrothermal pretreatment for rice straw	0:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	3	17	contain	containing	565:574	arg2	xylan					587:591	xylan	587:591	xylan	587:591	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	3	17	contain	containing	565:574	arg2	cellulose					576:584	cellulose	576:584	cellulose	576:584	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	3	17	contain	containing	565:574	arg2	lignin					598:603	lignin	598:603	lignin	598:603	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	3	17	contain	containing	565:574	arg1	mixture					557:563	the mixture	553:563	the mixture containing cellulose, xylan, and lignin	553:603	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	0	18	theme	hydrothermal	21:32	arg1	pretreatment					34:45	hydrothermal pretreatment	21:45	hydrothermal pretreatment for rice straw	21:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	1	19	from	production	259:268	arg1	terms					288:292	terms	288:292	terms of structural cross-linking between cellulose and the above components	288:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	5	20	theme	hydrothermal	948:959	arg1	pretreatment					961:972	hydrothermal pretreatment	948:972	hydrothermal pretreatment	948:972	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	1	21	theme	lignin	180:185	arg1	effect					152:157	the effect	148:157	the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	148:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	6	22	theme	hydrothermal	1127:1138	arg1	conditions					1140:1149	hydrothermal conditions	1127:1149	hydrothermal conditions	1127:1149	A model was therefore proposed to account for the obtained result in accordance with previous knowledge regarding the behavior of these compounds under hydrothermal conditions.
29414153	4	23	theme	enzymatic	738:746	arg1	hydrolysis					748:757	enzymatic hydrolysis	738:757	enzymatic hydrolysis	738:757	The obtained products were then subjected to enzymatic hydrolysis.
29414153	2	24	theme	biomass	429:435	arg1	pretreatment					413:424	the hydrothermal pretreatment	396:424	the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio	396:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	0	25	theme	pretreatment	34:45	arg1	mixture					84:90	its corresponding mixture	66:90	its corresponding mixture of cellulose, xylan, and lignin	66:122	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	0	25	theme	pretreatment	34:45	arg1	study					12:16	Comparative study	0:16	Comparative study of hydrothermal pretreatment for rice straw	0:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	1	26	theme	structural	297:306	arg1	cross-linking					308:320	structural cross-linking	297:320	structural cross-linking between cellulose and the above components	297:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	0	27	theme	lignin	117:122	arg1	mixture					84:90	its corresponding mixture	66:90	its corresponding mixture of cellulose, xylan, and lignin	66:122	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	0	27	theme	lignin	117:122	arg1	study					12:16	Comparative study	0:16	Comparative study of hydrothermal pretreatment for rice straw	0:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	3	28	theme	autoclave	624:632	arg1	reactor					634:640	an autoclave reactor	621:640	an autoclave reactor	621:640	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	2	29	theme	mixture	443:449	arg1	pretreatment					413:424	the hydrothermal pretreatment	396:424	the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio	396:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	5	30	from	presence	835:842	arg1	sample					914:919	the original biomass sample	893:919	the original biomass sample	893:919	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	1	31	theme	hydrothermal	194:205	arg1	pretreatment					207:218	the hydrothermal pretreatment	190:218	the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	190:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	2	32	theme	model	477:481	arg1	compounds					483:491	the individual model compounds	462:491	the individual model compounds	462:491	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	1	33	theme	cross-linking	308:320	arg1	terms					288:292	terms	288:292	terms of structural cross-linking between cellulose and the above components	288:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	5	34	theme	biomass	906:912	arg1	sample					914:919	the original biomass sample	893:919	the original biomass sample	893:919	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	0	35	theme	rice	51:54	arg1	straw					56:60	rice straw	51:60	rice straw	51:60	Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin.
29414153	4	36	theme	obtained	697:704	arg1	products					706:713	The obtained products	693:713	The obtained products	693:713	The obtained products were then subjected to enzymatic hydrolysis.
29414153	6	37	theme	compounds	1111:1119	arg1	behavior					1093:1100	the behavior	1089:1100	the behavior of these compounds under hydrothermal conditions	1089:1149	A model was therefore proposed to account for the obtained result in accordance with previous knowledge regarding the behavior of these compounds under hydrothermal conditions.
29414153	6	38	theme	obtained	1025:1032	arg1	result					1034:1039	the obtained result	1021:1039	the obtained result	1021:1039	A model was therefore proposed to account for the obtained result in accordance with previous knowledge regarding the behavior of these compounds under hydrothermal conditions.
29414153	5	39	attach	presence	835:842	arg1	sample					914:919	the original biomass sample	893:919	the original biomass sample	893:919	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	5	39	attach	presence	835:842	arg2	cross-linking					847:859	cross-linking	847:859	cross-linking between cellulose and lignin	847:888	Interestingly, different results were obtained for the two samples, as the presence of cross-linking between cellulose and lignin in the original biomass sample affected the efficiency of hydrothermal pretreatment.
29414153	6	40	theme	previous	1060:1067	arg1	knowledge					1069:1077	previous knowledge	1060:1077	previous knowledge regarding the behavior of these compounds under hydrothermal conditions	1060:1149	A model was therefore proposed to account for the obtained result in accordance with previous knowledge regarding the behavior of these compounds under hydrothermal conditions.
29414153	1	41	theme	lignocellulosic	223:237	arg1	biomass					239:245	lignocellulosic biomass	223:245	lignocellulosic biomass	223:245	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	2	42	theme	composition	505:515	arg1	ratio					517:521	the same composition ratio	496:521	the same composition ratio	496:521	A comparison was made between the hydrothermal pretreatment of biomass and a mixture containing the individual model compounds in the same composition ratio.
29414153	1	43	from	effect	152:157	arg1	pretreatment					207:218	the hydrothermal pretreatment	190:218	the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	190:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
29414153	3	44	theme	rice	530:533	arg1	biomass					541:547	rice straw biomass	530:547	rice straw biomass	530:547	Thus, rice straw biomass and the mixture containing cellulose, xylan, and lignin were treated in an autoclave reactor at temperatures between 150 and 250 °C for 30 min.
29414153	1	45	theme	biomass	239:245	arg1	pretreatment					207:218	the hydrothermal pretreatment	190:218	the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components	190:363	We herein investigated the effect of hemicellulose and lignin on the hydrothermal pretreatment of lignocellulosic biomass for glucose production from cellulose in terms of structural cross-linking between cellulose and the above components.
31151519	0	0	theme	human	95:99	arg1	cells					118:122	human mesenchymal stem cells	95:122	human mesenchymal stem cells	95:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	1	theme	osteogenic	719:728	arg1	markers					730:736	osteogenic markers	719:736	osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein)	719:786	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	2	theme	umbilical	616:624	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	2	theme	umbilical	616:624	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	6	3	theme	PKH-26-labelled	1003:1017	arg1	cells					1019:1023	many PKH-26-labelled cells	998:1023	many PKH-26-labelled cells	998:1023	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	1	4	theme	stem	159:162	arg1	differentiation					169:183	stem cell differentiation	159:183	stem cell differentiation	159:183	Scaffold plays a critical role in stem cell differentiation and tissue regeneration.
31151519	5	5	theme	cord	626:629	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	5	theme	cord	626:629	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	1	6	theme	cell	164:167	arg1	differentiation					169:183	stem cell differentiation	159:183	stem cell differentiation	159:183	Scaffold plays a critical role in stem cell differentiation and tissue regeneration.
31151519	3	7	dep	uptake	509:514	arg1	%					525:525	%	525:525	%	525:525	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	0	8	theme	stem	113:116	arg1	cells					118:122	human mesenchymal stem cells	95:122	human mesenchymal stem cells	95:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	0	9	from	Fabrication	0:10	arg1	cells					118:122	human mesenchymal stem cells	95:122	human mesenchymal stem cells	95:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	10	theme	blood	631:635	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	10	theme	blood	631:635	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	0	11	theme	mesenchymal	101:111	arg1	cells					118:122	human mesenchymal stem cells	95:122	human mesenchymal stem cells	95:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	12	theme	derived	637:643	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	12	theme	derived	637:643	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	6	13	theme	muscle	1058:1063	arg1	actin					1065:1069	α-smooth muscle actin	1049:1069	α-smooth muscle actin	1049:1069	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	3	14	dep	400	522:524	arg1	to					519:520	to	519:520	to	519:520	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	3	15	theme	Col	480:482	arg1	scaffold					484:491	Col scaffold	480:491	Col scaffold	480:491	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	5	16	link	derived	637:643	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	16	link	derived	637:643	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	3	17	theme	well-organized	387:400	arg1	structure					424:432	a well-organized interconnected porous structure	385:432	a well-organized interconnected porous structure	385:432	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	3	18	theme	porous	417:422	arg1	structure					424:432	a well-organized interconnected porous structure	385:432	a well-organized interconnected porous structure	385:432	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	5	19	theme	osteogenic	592:601	arg1	induction					603:611	osteogenic induction	592:611	osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks	592:689	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	6	20	theme	α-smooth	1049:1056	arg1	actin					1065:1069	α-smooth muscle actin	1049:1069	α-smooth muscle actin	1049:1069	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	7	21	theme	current	1120:1126	arg1	work					1128:1131	The current work	1116:1131	The current work	1116:1131	The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
31151519	5	22	dep	found	695:699	arg1	up-regulated					706:717	up-regulated	706:717	found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold	695:886	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	1	23	theme	tissue	189:194	arg1	regeneration					196:207	tissue regeneration	189:207	tissue regeneration	189:207	Scaffold plays a critical role in stem cell differentiation and tissue regeneration.
31151519	5	24	theme	elevated	806:813	arg1	proteins					815:822	significantly elevated proteins	792:822	significantly elevated proteins	792:822	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	3	25	theme	interconnected	402:415	arg1	structure					424:432	a well-organized interconnected porous structure	385:432	a well-organized interconnected porous structure	385:432	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	0	26	theme	cellulose-collagen	25:42	arg1	scaffolds					54:62	bacterial cellulose-collagen composite scaffolds	15:62	bacterial cellulose-collagen composite scaffolds	15:62	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	27	theme	BC/Col	866:871	arg1	scaffold					879:886	BC/Col (5:1) scaffold	866:886	BC/Col (5:1) scaffold	866:886	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	3	28	theme	better	449:454	arg1	stability					465:473	significantly better physical stability	435:473	significantly better physical stability than Col scaffold	435:491	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	0	29	theme	bacterial	15:23	arg1	scaffolds					54:62	bacterial cellulose-collagen composite scaffolds	15:62	bacterial cellulose-collagen composite scaffolds	15:62	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	6	30	theme	MSC-loaded	914:923	arg1	scaffolds					925:933	PKH-26 pre-labelled MSC-loaded scaffolds	894:933	PKH-26 pre-labelled MSC-loaded scaffolds	894:933	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	5	31	dep	markers	730:736	arg1	type					748:751	collagen type 1	739:753	collagen type 1	739:753	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	31	dep	markers	730:736	arg1	osteocalcin					756:766	osteocalcin	756:766	osteocalcin	756:766	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	31	dep	markers	730:736	arg1	sialoprotein					774:785	bone sialoprotein	769:785	bone sialoprotein	769:785	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	4	32	dep	cell	558:561	arg1	attachment					563:572	attachment	563:572	attachment	563:572	They were also favorable with cell attachment and growth.
31151519	2	33	theme	different	289:297	arg1	ratios					299:304	different ratios	289:304	different ratios (1:1, 3:1, 5:1)	289:320	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	3	34	theme	physical	456:463	arg1	stability					465:473	significantly better physical stability	435:473	significantly better physical stability than Col scaffold	435:491	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	0	35	theme	scaffolds	54:62	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bacterial cellulose-collagen composite scaffolds	0:62	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	0	35	theme	scaffolds	54:62	arg1	effect					85:90	their osteogenic effect	68:90	their osteogenic effect on human mesenchymal stem cells	68:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	6	36	theme	pre-labelled	901:912	arg1	scaffolds					925:933	PKH-26 pre-labelled MSC-loaded scaffolds	894:933	PKH-26 pre-labelled MSC-loaded scaffolds	894:933	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	7	37	theme	bone	1195:1198	arg1	scaffold					1218:1225	a bone tissue-engineered scaffold	1193:1225	a bone tissue-engineered scaffold	1193:1225	The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
31151519	5	38	theme	calcium	828:834	arg1	deposition					836:845	calcium deposition	828:845	calcium deposition	828:845	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	6	39	theme	many	998:1001	arg1	cells					1019:1023	many PKH-26-labelled cells	998:1023	many PKH-26-labelled cells	998:1023	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	0	40	theme	composite	44:52	arg1	scaffolds					54:62	bacterial cellulose-collagen composite scaffolds	15:62	bacterial cellulose-collagen composite scaffolds	15:62	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	41	theme	collagen	739:746	arg1	type					748:751	collagen type 1	739:753	collagen type 1	739:753	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	41	theme	collagen	739:746	arg1	osteocalcin					756:766	osteocalcin	756:766	osteocalcin	756:766	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	41	theme	collagen	739:746	arg1	sialoprotein					774:785	bone sialoprotein	769:785	bone sialoprotein	769:785	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	0	42	from	effect	85:90	arg1	cells					118:122	human mesenchymal stem cells	95:122	human mesenchymal stem cells	95:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	6	43	from	neovascularization	1076:1093	arg1	5:1					1110:1112	5:1	1110:1112	5:1	1110:1112	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	6	43	from	neovascularization	1076:1093	arg1	BC/Col					1102:1107	the BC/Col	1098:1107	the BC/Col (5:1)	1098:1113	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	6	44	theme	positive	1029:1036	arg1	signals					1038:1044	positive signals	1029:1044	positive signals of α-smooth muscle actin	1029:1069	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	3	45	theme	composite	357:365	arg1	scaffolds					367:375	The composite scaffolds	353:375	The composite scaffolds	353:375	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	5	46	theme	mesenchymal	645:655	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	46	theme	mesenchymal	645:655	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	2	47	theme	bacterial	242:250	arg1	cellulose					252:260	bacterial cellulose	242:260	bacterial cellulose (BC)	242:265	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	2	47	theme	bacterial	242:250	arg1	BC					263:264	BC	263:264	BC	263:264	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	2	48	dep	ratios	299:304	arg1	5:1					317:319	5:1	317:319	5:1	317:319	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	2	48	dep	ratios	299:304	arg1	3:1					312:314	3:1	312:314	3:1	312:314	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	2	48	dep	ratios	299:304	arg1	1:1					307:309	1:1	307:309	1:1	307:309	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	5	49	theme	stem	657:660	arg1	cells					662:666	umbilical cord blood derived mesenchymal stem cells	616:666	umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)	616:677	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	49	theme	stem	657:660	arg1	UCB-MSCs					669:676	UCB-MSCs	669:676	UCB-MSCs	669:676	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	7	50	theme	BC/Col	1155:1160	arg1	composites					1162:1171	our BC/Col composites	1151:1171	our BC/Col composites	1151:1171	The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
31151519	7	51	theme	tissue-engineered	1200:1216	arg1	scaffold					1218:1225	a bone tissue-engineered scaffold	1193:1225	a bone tissue-engineered scaffold	1193:1225	The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
31151519	5	52	theme	cells	662:666	arg1	induction					603:611	osteogenic induction	592:611	osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks	592:689	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	2	53	theme	Composite	210:218	arg1	scaffolds					220:228	Composite scaffolds	210:228	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1)	210:320	Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study.
31151519	1	54	theme	critical	142:149	arg1	role					151:154	a critical role	140:154	a critical role	140:154	Scaffold plays a critical role in stem cell differentiation and tissue regeneration.
31151519	6	55	theme	actin	1065:1069	arg1	cells					1019:1023	many PKH-26-labelled cells	998:1023	many PKH-26-labelled cells	998:1023	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	6	55	theme	actin	1065:1069	arg1	signals					1038:1044	positive signals	1029:1044	positive signals of α-smooth muscle actin	1029:1069	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
31151519	4	56	with	favorable	543:551	arg1	cell					558:561	cell attachment and growth	558:583	cell	558:561	They were also favorable with cell attachment and growth.
31151519	4	56	with	favorable	543:551	arg1	growth					578:583	growth	578:583	growth	578:583	They were also favorable with cell attachment and growth.
31151519	3	57	dep	%	525:525	arg1	400					522:524	400	522:524	400	522:524	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	0	58	theme	osteogenic	74:83	arg1	effect					85:90	their osteogenic effect	68:90	their osteogenic effect on human mesenchymal stem cells	68:122	Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells.
31151519	5	59	theme	bone	769:772	arg1	type					748:751	collagen type 1	739:753	collagen type 1	739:753	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	5	59	theme	bone	769:772	arg1	sialoprotein					774:785	bone sialoprotein	769:785	bone sialoprotein	769:785	After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold.
31151519	3	60	theme	water	503:507	arg1	uptake					509:514	more water uptake	498:514	more water uptake up to 400%	498:525	The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%.
31151519	6	61	theme	mouse	973:977	arg1	model					979:983	a mouse model	971:983	a mouse model	971:983	When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1).
30174062	0	0	theme	film	81:84	arg1	Effect					0:5	Effect	0:5	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.	0:181	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	7	1	with	comparison	828:837	arg1	control					848:854	control	848:854	control	848:854	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	7	1	with	comparison	828:837	arg1	fruit					872:876	ABA-treated fruit	860:876	ABA-treated fruit	860:876	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	3	2	theme	color	201:205	arg1	change					207:212	The color change	197:212	The color change of the jujube fruit after harvest	197:246	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	3	2	theme	color	201:205	arg1	indicator					269:277	a quality indicator	259:277	a quality indicator	259:277	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	6	3	theme	positive	691:698	arg1	correlation					700:710	A significant positive correlation	677:710	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content	677:785	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	0	4	theme	winter	146:151	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	5	theme	composite	71:79	arg1	film					81:84	chitosan/nano-silica/sodium alginate composite film	34:84	chitosan/nano-silica/sodium alginate composite film	34:84	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	6	theme	Zizyphus	161:168	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	4	7	theme	composite	358:366	arg1	film					368:371	a chitosan/nano-silica/sodium alginate composite film	319:371	a chitosan/nano-silica/sodium alginate composite film	319:371	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	8	theme	jujuba	170:175	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	9	from	Effect	0:5	arg1	quality					115:121	quality	115:121	quality	115:121	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	9	from	Effect	0:5	arg1	development					99:109	color development	93:109	color development	93:109	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	7	10	theme	polyphenol	907:916	arg1	PPO					927:929	PPO	927:929	PPO	927:929	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	7	10	theme	polyphenol	907:916	arg1	oxidase					918:924	polyphenol oxidase	907:924	polyphenol oxidase (PPO)	907:930	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	4	11	theme	alginate	349:356	arg1	film					368:371	a chitosan/nano-silica/sodium alginate composite film	319:371	a chitosan/nano-silica/sodium alginate composite film	319:371	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	12	dep	development	99:109	arg1	the					89:91	the	89:91	the	89:91	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	5	13	theme	composite	508:516	arg1	film					518:521	a composite film	506:521	a composite film	506:521	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	6	14	theme	water	738:742	arg1	loss					744:747	water loss	738:747	water loss	738:747	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	0	15	theme	Mill	177:180	arg1	quality					115:121	quality	115:121	quality	115:121	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	15	theme	Mill	177:180	arg1	development					99:109	color development	93:109	color development	93:109	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	5	16	theme	jujube	577:582	arg1	life					547:550	the shelf life	537:550	the shelf life of post-harvested winter jujube	537:582	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	6	17	theme	significant	679:689	arg1	correlation					700:710	A significant positive correlation	677:710	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content	677:785	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	4	18	theme	jujube	438:443	arg1	development					386:396	color development	380:396	color development	380:396	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	18	theme	jujube	438:443	arg1	properties					414:423	qualitative properties	402:423	qualitative properties	402:423	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	3	19	theme	jujube	221:226	arg1	fruit					228:232	the jujube fruit	217:232	the jujube fruit	217:232	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	4	20	theme	film	368:371	arg1	effects					284:290	The effects	280:290	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage	280:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	21	theme	color	93:97	arg1	development					99:109	color development	93:109	color development	93:109	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	3	22	theme	fruit	228:232	arg1	change					207:212	The color change	197:212	The color change of the jujube fruit after harvest	197:246	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	3	22	theme	fruit	228:232	arg1	indicator					269:277	a quality indicator	259:277	a quality indicator	259:277	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	6	23	theme	malondialdehyde	757:771	arg1	content					779:785	the malondialdehyde (MDA) content	753:785	the malondialdehyde (MDA) content	753:785	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	4	24	theme	cold	448:451	arg1	storage					453:459	cold storage	448:459	cold storage	448:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	7	25	theme	composite	991:999	arg1	fruit					1014:1018	a composite film-treated fruit	989:1018	a composite film-treated fruit	989:1018	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	5	26	theme	shelf	541:545	arg1	life					547:550	the shelf life	537:550	the shelf life of post-harvested winter jujube	537:582	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	4	27	from	development	386:396	arg1	storage					453:459	cold storage	448:459	cold storage	448:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	28	from	properties	414:423	arg1	storage					453:459	cold storage	448:459	cold storage	448:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	29	from	jujube	438:443	arg1	storage					453:459	cold storage	448:459	cold storage	448:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	30	theme	chitosan/nano-silica/sodium	321:347	arg1	film					368:371	a chitosan/nano-silica/sodium alginate composite film	319:371	a chitosan/nano-silica/sodium alginate composite film	319:371	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	31	from	storage	453:459	arg1	development					386:396	color development	380:396	color development	380:396	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	31	from	storage	453:459	arg1	properties					414:423	qualitative properties	402:423	qualitative properties	402:423	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	5	32	theme	ABA	621:623	arg1	treatment					625:633	the ABA treatment	617:633	the ABA treatment	617:633	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	3	33	used	used	251:254	arg2	indicator					269:277	a quality indicator	259:277	a quality indicator	259:277	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	3	33	used	used	251:254	arg2	change					207:212	The color change	197:212	The color change of the jujube fruit after harvest	197:246	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	7	34	theme	superoxide	879:888	arg1	dismutase					890:898	superoxide dismutase	879:898	superoxide dismutase (SOD)	879:904	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	7	34	theme	superoxide	879:888	arg1	SOD					901:903	SOD	901:903	SOD	901:903	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	5	35	theme	post-harvested	555:568	arg1	jujube					577:582	post-harvested winter jujube	555:582	post-harvested winter jujube	555:582	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	0	36	theme	abscisic	10:17	arg1	ABA					25:27	ABA	25:27	ABA	25:27	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	0	36	theme	abscisic	10:17	arg1	acid					19:22	abscisic acid	10:22	abscisic acid (ABA)	10:28	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	8	37	from	gene	1087:1090	arg1	addition					1024:1031	addition	1024:1031	addition	1024:1031	In addition, the dihydroflavonol-4-reductase (DFR) is the primary gene that regulates the expression of anthocyanin.
30174062	7	38	theme	film-treated	1001:1012	arg1	fruit					1014:1018	a composite film-treated fruit	989:1018	a composite film-treated fruit	989:1018	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	8	39	theme	anthocyanin	1125:1135	arg1	expression					1111:1120	the expression	1107:1120	the expression of anthocyanin	1107:1135	In addition, the dihydroflavonol-4-reductase (DFR) is the primary gene that regulates the expression of anthocyanin.
30174062	0	40	theme	postharvest	126:136	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	4	41	theme	qualitative	402:412	arg1	properties					414:423	qualitative properties	402:423	qualitative properties	402:423	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	6	42	theme	a∗/b∗	724:728	arg1	values					730:735	the a∗/b∗ values	720:735	the a∗/b∗ values	720:735	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	4	43	theme	harvested	428:436	arg1	jujube					438:443	harvested jujube	428:443	harvested jujube in cold storage	428:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	44	theme	acid	19:22	arg1	Effect					0:5	Effect	0:5	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.	0:181	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	4	45	theme	acid	304:307	arg1	effects					284:290	The effects	280:290	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage	280:459	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	8	46	theme	primary	1079:1085	arg1	dihydroflavonol-4-reductase					1038:1064	the dihydroflavonol-4-reductase	1034:1064	the dihydroflavonol-4-reductase (DFR)	1034:1070	In addition, the dihydroflavonol-4-reductase (DFR) is the primary gene that regulates the expression of anthocyanin.
30174062	8	46	theme	primary	1079:1085	arg1	gene					1087:1090	the primary gene	1075:1090	the primary gene that regulates the expression of anthocyanin	1075:1135	In addition, the dihydroflavonol-4-reductase (DFR) is the primary gene that regulates the expression of anthocyanin.
30174062	6	47	from	fruits	801:806	arg1	storage					816:822	cold storage	811:822	cold storage	811:822	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	7	48	dep	control	848:854	arg1	the					844:846	the	844:846	the	844:846	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	3	49	theme	quality	261:267	arg1	change					207:212	The color change	197:212	The color change of the jujube fruit after harvest	197:246	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	3	49	theme	quality	261:267	arg1	indicator					269:277	a quality indicator	259:277	a quality indicator	259:277	The color change of the jujube fruit after harvest is used as a quality indicator.
30174062	4	50	theme	abscisic	295:302	arg1	acid					304:307	abscisic acid	295:307	abscisic acid (ABA)	295:313	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	50	theme	abscisic	295:302	arg1	ABA					310:312	ABA	310:312	ABA	310:312	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	51	theme	Chinese	138:144	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	4	52	theme	color	380:384	arg1	development					386:396	color development	380:396	color development	380:396	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	6	53	theme	cold	811:814	arg1	storage					816:822	cold storage	811:822	cold storage	811:822	A significant positive correlation between the a∗/b∗ values, water loss and the malondialdehyde (MDA) content was found for fruits in cold storage.
30174062	7	54	theme	ABA-treated	860:870	arg1	fruit					872:876	ABA-treated fruit	860:876	ABA-treated fruit	860:876	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	7	55	theme	dismutase	890:898	arg1	activities					953:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	5	56	theme	winter	570:575	arg1	jujube					577:582	post-harvested winter jujube	555:582	post-harvested winter jujube	555:582	The results indicate that a composite film could prolong the shelf life of post-harvested winter jujube for approximately 1 month, while the ABA treatment induced ripening and reduced the quality.
30174062	0	57	theme	jujube	153:158	arg1	Mill					177:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	postharvest Chinese winter jujube (Zizyphus jujuba Mill	126:180	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	4	58	from	effects	284:290	arg1	development					386:396	color development	380:396	color development	380:396	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	58	from	effects	284:290	arg1	properties					414:423	qualitative properties	402:423	qualitative properties	402:423	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	4	59	dep	development	386:396	arg1	the					376:378	the	376:378	the	376:378	The effects of abscisic acid (ABA) and a chitosan/nano-silica/sodium alginate composite film on the color development and qualitative properties of harvested jujube in cold storage were investigated.
30174062	0	60	theme	alginate	62:69	arg1	film					81:84	chitosan/nano-silica/sodium alginate composite film	34:84	chitosan/nano-silica/sodium alginate composite film	34:84	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	7	61	theme	peroxidase	936:945	arg1	activities					953:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30174062	0	62	theme	chitosan/nano-silica/sodium	34:60	arg1	film					81:84	chitosan/nano-silica/sodium alginate composite film	34:84	chitosan/nano-silica/sodium alginate composite film	34:84	Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill.
30174062	7	63	theme	oxidase	918:924	arg1	activities					953:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities	879:962	In comparison with the control and ABA-treated fruit, superoxide dismutase (SOD), polyphenol oxidase (PPO) and peroxidase (POD) activities were lower than those in a composite film-treated fruit.
30857960	6	0	theme	other	814:818	arg1	analysis					847:854	the other monosaccharide composition analysis	810:854	the other monosaccharide composition analysis	810:854	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	1	1	theme	microwave	133:141	arg1	pretreatment					143:154	microwave pretreatment	133:154	microwave pretreatment	133:154	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	5	2	dep	chromatographic	626:640	arg1	GC					643:644	GC	643:644	GC	643:644	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	5	3	dep	spectroscopic	697:709	arg1	transform					720:728	transform	720:728	transform infrared	720:737	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	8	4	from	industry	1181:1188	arg1	additive					1164:1171	additive	1164:1171	additive	1164:1171	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	8	4	from	industry	1181:1188	arg1	pectin					1122:1127	the pectin	1118:1127	the pectin of PMTL	1118:1135	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	2	5	theme	pectin	324:329	arg1	yield					342:346	the pectin extraction yield	320:346	the pectin extraction yield	320:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	6	6	from	mannose	932:938	arg1	ratio					968:972	a ratio	966:972	a ratio of 2.96:1.17:1.04:8.07:2.05	966:1000	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	8	7	used	used	1146:1149	arg2	pectin					1122:1127	the pectin	1118:1127	the pectin of PMTL	1118:1135	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	8	7	used	used	1146:1149	arg2	additive					1164:1171	additive	1164:1171	additive	1164:1171	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	7	8	contain	had	1022:1024	arg1	pectin					1007:1012	The pectin	1003:1012	The pectin of PMTL	1003:1020	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	7	8	contain	had	1022:1024	arg2	degree					1044:1049	an esterification degree	1026:1049	an esterification degree of 62.50%	1026:1059	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	7	9	theme	PMTL	1017:1020	arg1	pectin					1007:1012	The pectin	1003:1012	The pectin of PMTL	1003:1020	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	2	10	theme	extraction	331:340	arg1	yield					342:346	the pectin extraction yield	320:346	the pectin extraction yield	320:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	5	11	theme	spectrophotometric	648:665	arg1	methods					740:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	8	12	from	additive	1164:1171	arg1	industry					1181:1188	food industry	1176:1188	food industry	1176:1188	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	7	13	theme	antioxidant	1080:1090	arg1	activity					1092:1099	good antioxidant activity	1075:1099	good antioxidant activity	1075:1099	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	6	14	from	glucose	941:947	arg1	ratio					968:972	a ratio	966:972	a ratio of 2.96:1.17:1.04:8.07:2.05	966:1000	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	6	15	theme	2.96:1.17:1.04:8.07:2.05	977:1000	arg1	ratio					968:972	a ratio	966:972	a ratio of 2.96:1.17:1.04:8.07:2.05	966:1000	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	7	16	theme	good	1075:1078	arg1	activity					1092:1099	good antioxidant activity	1075:1099	good antioxidant activity	1075:1099	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	3	17	dep	follows	389:395	arg1	50 mL/g					471:477	ratio 50 mL/g	465:477	ratio 50 mL/g	465:477	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
30857960	2	18	theme	composite	292:300	arg1	design					302:307	the isovariant central composite design	269:307	the isovariant central composite design to improve the pectin extraction yield	269:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	3	19	theme	extraction	398:407	arg1	2 h					414:416	extraction time 2 h	398:416	extraction time 2 h	398:416	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
30857960	6	20	from	galactose	953:961	arg1	ratio					968:972	a ratio	966:972	a ratio of 2.96:1.17:1.04:8.07:2.05	966:1000	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	2	21	theme	central	284:290	arg1	design					302:307	the isovariant central composite design	269:307	the isovariant central composite design to improve the pectin extraction yield	269:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	1	22	theme	extract	169:175	arg1	pectin					177:182	extract pectin	169:182	extract pectin from Premna microphylla Turcz leaves (PMTL)	169:226	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	7	23	theme	%	1059:1059	arg1	degree					1044:1049	an esterification degree	1026:1049	an esterification degree of 62.50%	1026:1059	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	5	24	theme	gas	622:624	arg1	methods					740:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	2	25	theme	isovariant	273:282	arg1	design					302:307	the isovariant central composite design	269:307	the isovariant central composite design to improve the pectin extraction yield	269:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	2	26	dep	design	302:307	arg1	improve					312:318	improve	312:318	to improve the pectin extraction yield	309:346	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	5	27	theme	chromatographic	626:640	arg1	methods					740:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	1	28	theme	response	94:101	arg1	RSM					124:126	RSM	124:126	RSM	124:126	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	28	theme	response	94:101	arg1	methodology					111:121	response surface methodology	94:121	response surface methodology (RSM)	94:127	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	5	29	theme	spectroscopic	697:709	arg1	methods					740:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods	622:746	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	1	30	theme	Premna	189:194	arg1	microphylla					196:206	Premna microphylla Turcz leaves (PMTL)	189:226	Premna microphylla Turcz leaves (PMTL)	189:226	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	3	31	theme	time	409:412	arg1	2 h					414:416	extraction time 2 h	398:416	extraction time 2 h	398:416	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
30857960	3	32	theme	temperature	419:429	arg1	90 °C					431:435	temperature 90 °C	419:435	temperature 90 °C	419:435	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
30857960	0	33	theme	pectin	35:40	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	0	33	theme	pectin	35:40	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	4	34	theme	extraction	484:493	arg1	yield					495:499	The extraction yield	480:499	The extraction yield	480:499	The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%).
30857960	4	34	theme	extraction	484:493	arg1	%					510:510	18.25%	505:510	18.25%	505:510	The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%).
30857960	6	35	from	PMTL	885:888	arg1	pectin					873:878	pectin	873:878	pectin from PMTL	873:888	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	1	36	dep	Turcz	208:212	arg1	leaves					214:219	leaves	214:219	leaves	214:219	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	0	37	theme	microphylla	54:64	arg1	Turcz					66:70	Premna microphylla Turcz	47:70	Premna microphylla Turcz	47:70	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	5	38	dep	spectrophotometric	648:665	arg1	Spectroscopy					679:690	UV-visible Spectroscopy	668:690	UV-visible Spectroscopy	668:690	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	0	39	from	Turcz	66:70	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	0	39	from	Turcz	66:70	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	8	40	theme	PMTL	1132:1135	arg1	pectin					1122:1127	the pectin	1118:1127	the pectin of PMTL	1118:1135	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	8	40	theme	PMTL	1132:1135	arg1	additive					1164:1171	additive	1164:1171	additive	1164:1171	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	1	41	from	microphylla	196:206	arg1	pectin					177:182	extract pectin	169:182	extract pectin from Premna microphylla Turcz leaves (PMTL)	169:226	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	42	theme	surface	103:109	arg1	RSM					124:126	RSM	124:126	RSM	124:126	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	42	theme	surface	103:109	arg1	methodology					111:121	response surface methodology	94:121	response surface methodology (RSM)	94:127	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	0	43	theme	Premna	47:52	arg1	Turcz					66:70	Premna microphylla Turcz	47:70	Premna microphylla Turcz	47:70	Extraction and characterization of pectin from Premna microphylla Turcz leaves.
30857960	3	44	theme	ratio	465:469	arg1	50 mL/g					471:477	ratio 50 mL/g	465:477	ratio 50 mL/g	465:477	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
30857960	6	45	theme	composition	835:845	arg1	analysis					847:854	the other monosaccharide composition analysis	810:854	the other monosaccharide composition analysis	810:854	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	6	46	theme	acid	766:769	arg1	content					771:777	The galacturonic acid content	749:777	The galacturonic acid content	749:777	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	6	46	theme	acid	766:769	arg1	%					788:788	82.75%	783:788	82.75%	783:788	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	2	47	theme	process	233:239	arg1	variables					241:249	The process variables	229:249	The process variables	229:249	The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield.
30857960	6	48	theme	galacturonic	753:764	arg1	content					771:777	The galacturonic acid content	749:777	The galacturonic acid content	749:777	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	6	48	theme	galacturonic	753:764	arg1	%					788:788	82.75%	783:788	82.75%	783:788	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	1	49	dep	microphylla	196:206	arg1	Turcz					208:212	Turcz leaves	208:219	Premna microphylla Turcz leaves (PMTL)	189:226	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	49	dep	microphylla	196:206	arg1	PMTL					222:225	PMTL	222:225	PMTL	222:225	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	8	50	theme	food	1176:1179	arg1	industry					1181:1188	food industry	1176:1188	food industry	1176:1188	Taken together, the pectin of PMTL could be used as potential additive in food industry.
30857960	5	51	theme	UV-visible	668:677	arg1	Spectroscopy					679:690	UV-visible Spectroscopy	668:690	UV-visible Spectroscopy	668:690	Then the pectin was characterized by gas chromatographic (GC), spectrophotometric (UV-visible Spectroscopy) and spectroscopic (Fourier transform infrared) methods.
30857960	1	52	used	used	161:164	arg2	pretreatment					143:154	microwave pretreatment	133:154	microwave pretreatment	133:154	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	52	used	used	161:164	arg2	RSM					124:126	RSM	124:126	RSM	124:126	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	1	52	used	used	161:164	arg2	methodology					111:121	response surface methodology	94:121	response surface methodology (RSM)	94:127	In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL).
30857960	4	53	theme	predicted	559:567	arg1	value					569:573	the predicted value	555:573	the predicted value (17.60%)	555:582	The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%).
30857960	4	53	theme	predicted	559:567	arg1	%					581:581	17.60%	576:581	17.60%	576:581	The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%).
30857960	4	53	theme	predicted	559:567	arg1	conditions					524:533	these conditions	518:533	these conditions	518:533	The extraction yield was 18.25% under these conditions, which was close to the predicted value (17.60%).
30857960	7	54	theme	esterification	1029:1042	arg1	degree					1044:1049	an esterification degree	1026:1049	an esterification degree of 62.50%	1026:1059	The pectin of PMTL had an esterification degree of 62.50% and it showed good antioxidant activity.
30857960	6	55	theme	monosaccharide	820:833	arg1	analysis					847:854	the other monosaccharide composition analysis	810:854	the other monosaccharide composition analysis	810:854	The galacturonic acid content was 82.75%, and on this basis, the other monosaccharide composition analysis illustrated that pectin from PMTL was also composed of rhamnose, arabinose, mannose, glucose and galactose in a ratio of 2.96:1.17:1.04:8.07:2.05.
30857960	3	56	theme	optimum	353:359	arg1	conditions					361:370	The optimum conditions	349:370	The optimum conditions obtained	349:379	The optimum conditions obtained were as follows, extraction time 2 h, temperature 90 °C, pH 2 and liquid-solid (LS) ratio 50 mL/g.
31426303	3	0	dep	China	467:471	arg1	CYYS-2					405:410	CYYS-2	405:410	CYYS-2	405:410	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	0	dep	China	467:471	arg1	CYYS-3					417:422	CYYS-3	417:422	CYYS-3	417:422	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	0	dep	China	467:471	arg1	CYYS-1					397:402	CYYS-1	397:402	CYYS-1	397:402	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	0	dep	China	467:471	arg1	starches					387:394	starches	387:394	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town	387:439	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	8	1	theme	CYYS-1	888:893	arg1	temperature					873:883	The pasting temperature	861:883	The pasting temperature of CYYS-1	861:893	The pasting temperature of CYYS-1 increased significantly with sucrose addition.
31426303	6	2	theme	CYYS	706:709	arg1	stability					688:696	The freeze-thaw stability	672:696	The freeze-thaw stability of each CYYS	672:709	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	9	3	theme	CYYS	983:986	arg1	power					974:978	the swelling power	961:978	the swelling power of CYYS	961:986	NaCl could inhibit the swelling power of CYYS.
31426303	2	4	theme	paste	244:248	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	4	theme	paste	244:248	arg1	clarity					250:256	paste clarity	244:256	paste clarity	244:256	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	10	5	theme	significant	1000:1010	arg1	decreases					1012:1020	significant decreases	1000:1020	significant decreases in pasting temperature and pasting time of CYYS	1000:1068	There were significant decreases in pasting temperature and pasting time of CYYS when pH decreased.
31426303	2	6	dep	compare	150:156	arg1	Thunb					379:383	Thunb	379:383	Thunb	379:383	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	5	7	theme	30.34	607:611	arg1	%					602:602	%	602:602	%	602:602	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	6	8	theme	amylose	747:753	arg1	content					755:761	its amylose content	743:761	its amylose content	743:761	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	7	9	theme	highest	795:801	arg1	T					803:803	the highest T	791:803	the highest T (81.1 °C)	791:813	In addition, CYYS-3 showed the highest T (81.1 °C), T (84.8 °C), T (91.2 °C), and ΔH (14.1 J/g).
31426303	7	9	theme	highest	795:801	arg1	°C					811:812	81.1 °C	806:812	81.1 °C	806:812	In addition, CYYS-3 showed the highest T (81.1 °C), T (84.8 °C), T (91.2 °C), and ΔH (14.1 J/g).
31426303	8	10	theme	sucrose	924:930	arg1	addition					932:939	sucrose addition	924:939	sucrose addition	924:939	The pasting temperature of CYYS-1 increased significantly with sucrose addition.
31426303	2	11	theme	swelling	228:235	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	11	theme	swelling	228:235	arg1	power					237:241	swelling power	228:241	swelling power	228:241	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	4	12	theme	diffraction	537:547	arg1	pattern					549:555	a typical C-type X-ray diffraction pattern	514:555	a typical C-type X-ray diffraction pattern	514:555	Our results show that all the CYYS gave a typical C-type X-ray diffraction pattern.
31426303	9	13	theme	swelling	965:972	arg1	power					974:978	the swelling power	961:978	the swelling power of CYYS	961:986	NaCl could inhibit the swelling power of CYYS.
31426303	5	14	theme	%	669:669	arg1	range					651:655	the range	647:655	the range of 7.84-4.55%	647:669	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	6	15	theme	freeze-thaw	676:686	arg1	stability					688:696	The freeze-thaw stability	672:696	The freeze-thaw stability of each CYYS	672:709	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	6	16	theme	each	701:704	arg1	CYYS					706:709	each CYYS	701:709	each CYYS	701:709	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	4	17	theme	typical	516:522	arg1	pattern					549:555	a typical C-type X-ray diffraction pattern	514:555	a typical C-type X-ray diffraction pattern	514:555	Our results show that all the CYYS gave a typical C-type X-ray diffraction pattern.
31426303	0	18	theme	Physicochemical	16:30	arg1	Properties					32:41	Physicochemical Properties	16:41	Physicochemical Properties	16:41	Composition and Physicochemical Properties of Three Chinese Yam (Dioscorea opposita Thunb.)
31426303	6	19	theme	contrary	720:727	arg1	tendency					729:736	a contrary tendency	718:736	a contrary tendency with its amylose content	718:761	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	10	20	theme	CYYS	1065:1068	arg1	time					1057:1060	time	1057:1060	time of CYYS	1057:1068	There were significant decreases in pasting temperature and pasting time of CYYS when pH decreased.
31426303	2	21	theme	pasting	320:326	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	21	theme	pasting	320:326	arg1	property					328:335	pasting property	320:335	pasting property	320:335	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	5	22	theme	10.79	597:601	arg1	%					602:602	%	602:602	%	602:602	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	2	23	theme	yam	355:357	arg1	composition					162:172	the composition	158:172	the composition	158:172	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	23	theme	yam	355:357	arg1	properties					194:203	physicochemical properties	178:203	physicochemical properties	178:203	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	4	24	theme	X-ray	531:535	arg1	pattern					549:555	a typical C-type X-ray diffraction pattern	514:555	a typical C-type X-ray diffraction pattern	514:555	Our results show that all the CYYS gave a typical C-type X-ray diffraction pattern.
31426303	3	25	from	starches	387:394	arg1	town					436:439	Yunlong town	428:439	Yunlong town	428:439	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	2	26	theme	Chinese	347:353	arg1	yam					355:357	three Chinese yam	341:357	three Chinese yam	341:357	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	0	27	theme	Yam	60:62	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and Physicochemical Properties of Three Chinese Yam (Dioscorea opposita Thunb.)
31426303	0	27	theme	Yam	60:62	arg1	Properties					32:41	Physicochemical Properties	16:41	Physicochemical Properties	16:41	Composition and Physicochemical Properties of Three Chinese Yam (Dioscorea opposita Thunb.)
31426303	0	27	theme	Yam	60:62	arg1	Thunb					84:88	Thunb	84:88	Thunb	84:88	Composition and Physicochemical Properties of Three Chinese Yam (Dioscorea opposita Thunb.)
31426303	5	28	theme	swelling	562:569	arg1	power					571:575	The swelling power	558:575	The swelling power of CYYS	558:583	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	2	29	theme	work	138:141	arg1	aim					126:128	The aim	122:128	The aim of this work	122:141	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	30	theme	thermal	298:304	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	30	theme	thermal	298:304	arg1	property					306:313	thermal property	298:313	thermal property	298:313	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	0	31	theme	Chinese	52:58	arg1	Yam					60:62	Three Chinese Yam	46:62	Three Chinese Yam	46:62	Composition and Physicochemical Properties of Three Chinese Yam (Dioscorea opposita Thunb.)
31426303	2	32	theme	physicochemical	178:192	arg1	properties					194:203	physicochemical properties	178:203	physicochemical properties	178:203	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	1	33	theme	Comparison	104:113	arg1	Study					115:119	A Comparison Study	102:119	A Comparison Study	102:119	Starches: A Comparison Study.
31426303	8	34	theme	pasting	865:871	arg1	temperature					873:883	The pasting temperature	861:883	The pasting temperature of CYYS-1	861:893	The pasting temperature of CYYS-1 increased significantly with sucrose addition.
31426303	3	35	dep	starches	387:394	arg1	CYYS-2					405:410	CYYS-2	405:410	CYYS-2	405:410	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	35	dep	starches	387:394	arg1	CYYS-3					417:422	CYYS-3	417:422	CYYS-3	417:422	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	35	dep	starches	387:394	arg1	CYYS-1					397:402	CYYS-1	397:402	CYYS-1	397:402	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	3	35	dep	starches	387:394	arg1	starches					387:394	starches	387:394	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town	387:439	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	2	36	dep	composition	162:172	arg1	retrogradation					259:272	retrogradation	259:272	retrogradation	259:272	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	property					306:313	thermal property	298:313	thermal property	298:313	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	power					237:241	swelling power	228:241	swelling power	228:241	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	stability					287:295	freeze-thaw stability	275:295	freeze-thaw stability	275:295	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	XRD					211:213	XRD	211:213	XRD	211:213	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	property					328:335	pasting property	320:335	pasting property	320:335	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	solubility					216:225	solubility	216:225	solubility	216:225	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	36	dep	composition	162:172	arg1	clarity					250:256	paste clarity	244:256	paste clarity	244:256	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	5	37	theme	CYYS	580:583	arg1	power					571:575	The swelling power	558:575	The swelling power of CYYS	558:583	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	1	38	dep	Study	115:119	arg1	Starches					92:99	Starches	92:99	Starches	92:99	Starches: A Comparison Study.
31426303	2	39	theme	freeze-thaw	275:285	arg1	SEM					206:208	SEM	206:208	SEM	206:208	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	2	39	theme	freeze-thaw	275:285	arg1	stability					287:295	freeze-thaw stability	275:295	freeze-thaw stability	275:295	The aim of this work was to compare the composition and physicochemical properties (SEM, XRD, solubility, swelling power, paste clarity, retrogradation, freeze-thaw stability, thermal property, and pasting property) of three Chinese yam (Dioscorea opposita Thunb.)
31426303	4	40	theme	C-type	524:529	arg1	pattern					549:555	a typical C-type X-ray diffraction pattern	514:555	a typical C-type X-ray diffraction pattern	514:555	Our results show that all the CYYS gave a typical C-type X-ray diffraction pattern.
31426303	3	41	theme	Yunlong	428:434	arg1	town					436:439	Yunlong town	428:439	Yunlong town	428:439	starches (CYYS-1, CYYS-2, and CYYS-3) in Yunlong town, Haikou, Hainan Province, China.
31426303	5	42	theme	solubility	623:632	arg1	index					634:638	solubility index	623:638	solubility index	623:638	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
31426303	6	43	with	tendency	729:736	arg1	content					755:761	its amylose content	743:761	its amylose content	743:761	The freeze-thaw stability of each CYYS showed a contrary tendency with its amylose content.
31426303	5	44	dep	%	602:602	arg1	to					604:605	to	604:605	to	604:605	The swelling power of CYYS varied from 10.79% to 30.34%, whereas solubility index was in the range of 7.84-4.55%.
30865987	1	0	theme	human	146:150	arg1	milk					152:155	human milk	146:155	human milk	146:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	4	1	theme	characteristic	556:569	arg1	composition					575:585	a woman's characteristic HMO composition	546:585	a woman's characteristic HMO composition	546:585	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	5	2	theme	many	732:735	arg1	effects					737:743	the many effects	728:743	the many effects	728:743	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	1	3	theme	milk	152:155	arg1	oligosaccharides					81:96	Human milk oligosaccharides	70:96	Human milk oligosaccharides (HMOs)	70:103	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	3	theme	milk	152:155	arg1	component					133:141	the third most abundant component	109:141	the third most abundant component of human milk	109:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	6	4	theme	individual	815:824	arg1	HMOs					826:829	individual HMOs	815:829	individual HMOs exert a certain effect and, while there might be some redundancy, the effects	815:907	In some cases, individual HMOs exert a certain effect and, while there might be some redundancy, the effects are often highly structure-specific.
30865987	5	5	theme	studies	692:698	arg1	combination					643:653	A combination	641:653	A combination of preclinical, cohort, and clinical studies	641:698	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	3	6	theme	lactation	342:350	arg1	course					332:337	the course	328:337	the course of lactation in the same woman	328:368	HMO composition varies substantially between women, but remains fairly constant over the course of lactation in the same woman.
30865987	6	7	theme	certain	839:845	arg1	effect					847:852	a certain effect	837:852	a certain effect	837:852	In some cases, individual HMOs exert a certain effect and, while there might be some redundancy, the effects are often highly structure-specific.
30865987	4	8	theme	genetic	386:392	arg1	factors					412:418	maternal genetic and environmental factors	377:418	maternal genetic and environmental factors	377:418	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	1	9	theme	Human	70:74	arg1	HMOs					99:102	HMOs	99:102	HMOs	99:102	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	9	theme	Human	70:74	arg1	oligosaccharides					81:96	Human milk oligosaccharides	70:96	Human milk oligosaccharides (HMOs)	70:103	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	9	theme	Human	70:74	arg1	component					133:141	the third most abundant component	109:141	the third most abundant component of human milk	109:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	4	10	theme	HMO	571:573	arg1	composition					575:585	a woman's characteristic HMO composition	546:585	a woman's characteristic HMO composition	546:585	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	4	11	theme	HMO	460:462	arg1	composition					464:474	HMO composition	460:474	HMO composition	460:474	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	4	12	theme	maternal	377:384	arg1	factors					412:418	maternal genetic and environmental factors	377:418	maternal genetic and environmental factors	377:418	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	1	13	theme	milk	76:79	arg1	HMOs					99:102	HMOs	99:102	HMOs	99:102	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	13	theme	milk	76:79	arg1	oligosaccharides					81:96	Human milk oligosaccharides	70:96	Human milk oligosaccharides (HMOs)	70:103	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	13	theme	milk	76:79	arg1	component					133:141	the third most abundant component	109:141	the third most abundant component of human milk	109:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	7	14	theme	specific	997:1004	arg1	ratios					1006:1011	specific ratios	997:1011	specific ratios	997:1011	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	5	15	theme	potential	761:769	arg1	claims					771:776	potential claims	761:776	potential claims	761:776	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	4	16	theme	specific	625:632	arg1	infant					616:621	her own infant's specific needs	608:638	her own infant's specific needs	608:638	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	0	17	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides: Next-Generation Functions and Questions.
30865987	0	18	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides: Next-Generation Functions and Questions.
30865987	7	19	from	ratios	1006:1011	arg1	combination					964:974	a combination	962:974	a combination of different HMOs in specific ratios to each other	962:1025	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	19	from	ratios	1006:1011	arg1	effective					1045:1053	effective	1045:1053	effective	1045:1053	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	4	20	theme	environmental	398:410	arg1	factors					412:418	maternal genetic and environmental factors	377:418	maternal genetic and environmental factors	377:418	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	8	21	theme	personalized	1258:1269	arg1	complexity					1271:1280	the personalized complexity	1254:1280	the personalized complexity of HMOs	1254:1288	Overall, the personalized complexity of HMOs cannot be mimicked in artificial infant formula and provides yet another powerful reason to protect, promote, and support breastfeeding.
30865987	7	22	theme	long-term	1227:1235	arg1	health					1237:1242	the infant's short- and long-term health	1203:1242	the infant's short- and long-term health	1203:1242	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	5	23	theme	cohort	671:676	arg1	studies					692:698	preclinical, cohort, and clinical studies	658:698	preclinical, cohort, and clinical studies	658:698	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	4	24	theme	own	612:614	arg1	infant					616:621	her own infant's specific needs	608:638	her own infant's specific needs	608:638	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	7	25	theme	different	979:987	arg1	HMOs					989:992	different HMOs	979:992	different HMOs in specific ratios	979:1011	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	4	26	from	variations	446:455	arg1	composition					464:474	HMO composition	460:474	HMO composition	460:474	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	4	27	theme	interindividual	430:444	arg1	variations					446:455	the interindividual variations	426:455	the interindividual variations in HMO composition	426:474	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	8	28	theme	HMOs	1285:1288	arg1	complexity					1271:1280	the personalized complexity	1254:1280	the personalized complexity of HMOs	1254:1288	Overall, the personalized complexity of HMOs cannot be mimicked in artificial infant formula and provides yet another powerful reason to protect, promote, and support breastfeeding.
30865987	7	29	theme	short-	1216:1221	arg1	health					1237:1242	the infant's short- and long-term health	1203:1242	the infant's short- and long-term health	1203:1242	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	3	30	theme	HMO	243:245	arg1	composition					247:257	HMO composition	243:257	HMO composition	243:257	HMO composition varies substantially between women, but remains fairly constant over the course of lactation in the same woman.
30865987	3	31	from	course	332:337	arg1	woman					364:368	the same woman	355:368	the same woman	355:368	HMO composition varies substantially between women, but remains fairly constant over the course of lactation in the same woman.
30865987	7	32	theme	future	1060:1065	arg1	research					1067:1074	future research	1060:1074	future research	1060:1074	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	5	33	theme	clinical	683:690	arg1	studies					692:698	preclinical, cohort, and clinical studies	658:698	preclinical, cohort, and clinical studies	658:698	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	2	34	theme	distinct	207:214	arg1	HMOs					216:219	more than 150 different and structurally distinct HMOs	166:219	more than 150 different and structurally distinct HMOs	166:219	So far, more than 150 different and structurally distinct HMOs have been identified.
30865987	7	35	theme	other	949:953	arg1	cases					955:959	other cases	949:959	other cases	949:959	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	8	36	theme	powerful	1363:1370	arg1	reason					1372:1377	another powerful reason	1355:1377	another powerful reason to protect, promote, and support breastfeeding	1355:1424	Overall, the personalized complexity of HMOs cannot be mimicked in artificial infant formula and provides yet another powerful reason to protect, promote, and support breastfeeding.
30865987	8	37	theme	artificial	1312:1321	arg1	formula					1330:1336	artificial infant formula	1312:1336	artificial infant formula	1312:1336	Overall, the personalized complexity of HMOs cannot be mimicked in artificial infant formula and provides yet another powerful reason to protect, promote, and support breastfeeding.
30865987	7	38	theme	HMOs	989:992	arg1	combination					964:974	a combination	962:974	a combination of different HMOs in specific ratios to each other	962:1025	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	38	theme	HMOs	989:992	arg1	effective					1045:1053	effective	1045:1053	effective	1045:1053	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	2	39	theme	different	180:188	arg1	HMOs					216:219	more than 150 different and structurally distinct HMOs	166:219	more than 150 different and structurally distinct HMOs	166:219	So far, more than 150 different and structurally distinct HMOs have been identified.
30865987	0	40	dep	Next-Generation	29:43	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides: Next-Generation Functions and Questions.
30865987	0	40	dep	Next-Generation	29:43	arg1	Functions					45:53	Functions	45:53	Functions	45:53	Human Milk Oligosaccharides: Next-Generation Functions and Questions.
30865987	1	41	theme	third	113:117	arg1	oligosaccharides					81:96	Human milk oligosaccharides	70:96	Human milk oligosaccharides (HMOs)	70:103	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	41	theme	third	113:117	arg1	component					133:141	the third most abundant component	109:141	the third most abundant component of human milk	109:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	5	42	theme	preclinical	658:668	arg1	studies					692:698	preclinical, cohort, and clinical studies	658:698	preclinical, cohort, and clinical studies	658:698	A combination of preclinical, cohort, and clinical studies is required to fully assess the many effects, functions, and potential claims associated with HMOs.
30865987	4	43	dep	infant	616:621	arg1	needs					634:638	needs	634:638	needs	634:638	Which maternal genetic and environmental factors drive the interindividual variations in HMO composition remains poorly understood, and it is currently unknown whether or not a woman's characteristic HMO composition has evolved to match her own infant's specific needs.
30865987	8	44	theme	infant	1323:1328	arg1	formula					1330:1336	artificial infant formula	1312:1336	artificial infant formula	1312:1336	Overall, the personalized complexity of HMOs cannot be mimicked in artificial infant formula and provides yet another powerful reason to protect, promote, and support breastfeeding.
30865987	1	45	theme	abundant	124:131	arg1	oligosaccharides					81:96	Human milk oligosaccharides	70:96	Human milk oligosaccharides (HMOs)	70:103	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	1	45	theme	abundant	124:131	arg1	component					133:141	the third most abundant component	109:141	the third most abundant component of human milk	109:155	Human milk oligosaccharides (HMOs) are the third most abundant component of human milk.
30865987	6	46	dep	HMOs	826:829	arg1	exert					831:835	exert	831:835	exert a certain effect	831:852	In some cases, individual HMOs exert a certain effect and, while there might be some redundancy, the effects are often highly structure-specific.
30865987	7	47	theme	individual	1129:1138	arg1	HMOs					1140:1143	individual HMOs	1129:1143	individual HMOs alone	1129:1149	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	48	from	HMOs	989:992	arg1	ratios					1006:1011	specific ratios	997:1011	specific ratios	997:1011	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	49	from	combination	964:974	arg1	ratios					1006:1011	specific ratios	997:1011	specific ratios	997:1011	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	50	theme	HMOs	1140:1143	arg1	counterproductive					1158:1174	counterproductive	1158:1174	counterproductive	1158:1174	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	7	50	theme	HMOs	1140:1143	arg1	administration					1111:1124	the administration	1107:1124	the administration of individual HMOs alone	1107:1149	In other cases, a combination of different HMOs in specific ratios to each other is required to be effective, and future research needs to assess whether or not the administration of individual HMOs alone may be counterproductive and potentially harmful to the infant's short- and long-term health.
30865987	3	51	theme	same	359:362	arg1	woman					364:368	the same woman	355:368	the same woman	355:368	HMO composition varies substantially between women, but remains fairly constant over the course of lactation in the same woman.
29808729	3	0	theme	different	312:320	arg1	levels					322:327	different levels	312:327	different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour)	312:384	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	5	1	theme	xanthan	794:800	arg1	gum					802:804	xanthan gum	794:804	the xanthan gum level	790:810	Increasing the xanthan gum level increases the hardness and elasticity of the dough, and decreases its springiness, cohesiveness, and adhesiveness.
29808729	9	2	theme	dough	1403:1407	arg1	properties					1389:1398	the proper technological properties	1364:1398	the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1364:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	3	3	theme	levels	322:327	arg1	effect					302:307	The effect	298:307	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit	298:543	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	4	4	theme	biscuits	769:776	arg1	texture					716:722	the texture	712:722	the texture	712:722	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	4	theme	biscuits	769:776	arg1	aw					743:744	aw	743:744	aw	743:744	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	4	theme	biscuits	769:776	arg1	dimensions					751:760	dimensions	751:760	dimensions	751:760	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	4	theme	biscuits	769:776	arg1	weight					725:730	weight	725:730	weight	725:730	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	4	theme	biscuits	769:776	arg1	moisture					733:740	moisture	733:740	moisture	733:740	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	4	theme	biscuits	769:776	arg1	properties					676:685	the textural and linear viscoelastic properties	639:685	the textural and linear viscoelastic properties of the dough	639:698	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	8	5	theme	acceptability	1192:1204	arg1	terms					1175:1179	terms	1175:1179	terms of sensory acceptability	1175:1204	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	7	6	theme	moisture	1092:1099	arg1	content					1101:1107	the moisture content	1088:1107	the moisture content	1088:1107	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	5	7	theme	dough	857:861	arg1	elasticity					839:848	elasticity	839:848	elasticity	839:848	Increasing the xanthan gum level increases the hardness and elasticity of the dough, and decreases its springiness, cohesiveness, and adhesiveness.
29808729	5	7	theme	dough	857:861	arg1	hardness					826:833	hardness	826:833	hardness	826:833	Increasing the xanthan gum level increases the hardness and elasticity of the dough, and decreases its springiness, cohesiveness, and adhesiveness.
29808729	9	8	theme	viable	1497:1502	arg1	gum					1349:1351	using xanthan gum	1335:1351	using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1335:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	9	8	theme	viable	1497:1502	arg1	alternative					1504:1514	a viable alternative	1495:1514	a viable alternative	1495:1514	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	9	9	theme	biscuits	1483:1490	arg1	production					1437:1446	the industrial production	1422:1446	the industrial production of gluten-free rice-chickpea flour biscuits	1422:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	2	10	theme	rice-chickpea	277:289	arg1	flour					291:295	the rice-chickpea flour	273:295	the rice-chickpea flour	273:295	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	7	11	theme	baked	1127:1131	arg1	biscuits					1133:1140	the baked biscuits	1123:1140	the baked biscuits	1123:1140	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	3	12	theme	water	483:487	arg1	aw					499:500	aw	499:500	aw	499:500	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	12	theme	water	483:487	arg1	activity					489:496	water activity	483:496	water activity (aw)	483:501	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	13	theme	dough	435:439	arg1	texture					393:399	the texture	389:399	the texture	389:399	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	14	theme	gum	340:342	arg1	incorporation					344:356	xanthan gum incorporation	332:356	xanthan gum incorporation (0.5, 1, and 1.5% of flour)	332:384	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	8	15	theme	flour	1250:1254	arg1	acceptability					1264:1276	rice-chickpea flour biscuit acceptability	1236:1276	rice-chickpea flour biscuit acceptability	1236:1276	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	4	16	theme	xanthan	579:585	arg1	gum					587:589	xanthan gum	579:589	xanthan gum	579:589	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	3	17	theme	incorporation	344:356	arg1	levels					322:327	different levels	312:327	different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour)	312:384	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	5	18	theme	gum	802:804	arg1	level					806:810	the xanthan gum level	790:810	the xanthan gum level	790:810	Increasing the xanthan gum level increases the hardness and elasticity of the dough, and decreases its springiness, cohesiveness, and adhesiveness.
29808729	8	19	theme	rice-chickpea	1236:1248	arg1	acceptability					1264:1276	rice-chickpea flour biscuit acceptability	1236:1276	rice-chickpea flour biscuit acceptability	1236:1276	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	6	20	theme	significant	969:979	arg1	improvement					981:991	a significant improvement	967:991	a significant improvement in the thickness and specific volume of biscuits	967:1040	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	9	21	theme	xanthan	1341:1347	arg1	gum					1349:1351	using xanthan gum	1335:1351	using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1335:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	9	21	theme	xanthan	1341:1347	arg1	alternative					1504:1514	a viable alternative	1495:1514	a viable alternative	1495:1514	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	7	22	theme	holding	1061:1067	arg1	capacity					1069:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	9	23	theme	industrial	1426:1435	arg1	production					1437:1446	the industrial production	1422:1446	the industrial production of gluten-free rice-chickpea flour biscuits	1422:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	1	24	theme	gluten-free	79:89	arg1	biscuit					91:97	A gluten-free biscuit	77:97	A gluten-free biscuit for celiac children based on composite rice-chickpea flour	77:156	A gluten-free biscuit for celiac children based on composite rice-chickpea flour was developed.
29808729	7	25	theme	biscuit	1154:1160	arg1	hardness					1162:1169	biscuit hardness	1154:1169	biscuit hardness	1154:1169	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	7	26	theme	Xanthan	1043:1049	arg1	gum					1051:1053	Xanthan gum	1043:1053	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	4	27	theme	viscoelastic	663:674	arg1	properties					676:685	the textural and linear viscoelastic properties	639:685	the textural and linear viscoelastic properties of the dough	639:698	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	2	28	from	absence	252:258	arg1	flour					291:295	the rice-chickpea flour	273:295	the rice-chickpea flour	273:295	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	4	29	theme	gum	587:589	arg1	incorporation					562:574	The incorporation	558:574	The incorporation of xanthan gum into rice-chickpea flour	558:614	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	7	30	theme	gum	1051:1053	arg1	capacity					1069:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	9	31	theme	using	1335:1339	arg1	gum					1349:1351	using xanthan gum	1335:1351	using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1335:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	9	31	theme	using	1335:1339	arg1	alternative					1504:1514	a viable alternative	1495:1514	a viable alternative	1495:1514	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	6	32	theme	biscuits	1033:1040	arg1	thickness					1000:1008	thickness	1000:1008	thickness	1000:1008	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	6	32	theme	biscuits	1033:1040	arg1	volume					1023:1028	specific volume	1014:1028	specific volume	1014:1028	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	9	33	dep	gum	1349:1351	arg1	achieve					1356:1362	achieve	1356:1362	to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1353:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	6	34	theme	xanthan	943:949	arg1	gum					951:953	xanthan gum	943:953	xanthan gum	943:953	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	7	35	theme	water	1055:1059	arg1	capacity					1069:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity	1043:1076	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	9	36	theme	rice-chickpea	1463:1475	arg1	biscuits					1483:1490	gluten-free rice-chickpea flour biscuits	1451:1490	gluten-free rice-chickpea flour biscuits	1451:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	4	37	theme	dough	694:698	arg1	texture					716:722	the texture	712:722	the texture	712:722	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	37	theme	dough	694:698	arg1	aw					743:744	aw	743:744	aw	743:744	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	37	theme	dough	694:698	arg1	dimensions					751:760	dimensions	751:760	dimensions	751:760	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	37	theme	dough	694:698	arg1	weight					725:730	weight	725:730	weight	725:730	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	37	theme	dough	694:698	arg1	moisture					733:740	moisture	733:740	moisture	733:740	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	4	37	theme	dough	694:698	arg1	properties					676:685	the textural and linear viscoelastic properties	639:685	the textural and linear viscoelastic properties of the dough	639:698	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	1	38	theme	celiac	103:108	arg1	children					110:117	celiac children	103:117	celiac children based on composite rice-chickpea flour	103:156	A gluten-free biscuit for celiac children based on composite rice-chickpea flour was developed.
29808729	7	39	theme	biscuits	1133:1140	arg1	content					1101:1107	the moisture content	1088:1107	the moisture content	1088:1107	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	7	39	theme	biscuits	1133:1140	arg1	aw					1117:1118	the aw	1113:1118	the aw of the baked biscuits	1113:1140	Xanthan gum water holding capacity increases the moisture content and the aw of the baked biscuits and reduces biscuit hardness.
29808729	0	40	theme	rice-chickpea	40:52	arg1	flour					54:58	composite rice-chickpea flour	30:58	composite rice-chickpea flour	30:58	Gluten-free biscuits based on composite rice-chickpea flour and xanthan gum.
29808729	8	41	theme	xanthan	1207:1213	arg1	gum					1215:1217	xanthan gum	1207:1217	xanthan gum	1207:1217	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	3	42	theme	sensory	508:514	arg1	acceptability					516:528	sensory acceptability	508:528	sensory acceptability	508:528	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	6	43	theme	gum	951:953	arg1	addition					931:938	The addition	927:938	The addition of xanthan gum	927:953	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	9	44	theme	proper	1368:1373	arg1	properties					1389:1398	the proper technological properties	1364:1398	the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1364:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	0	45	theme	composite	30:38	arg1	flour					54:58	composite rice-chickpea flour	30:58	composite rice-chickpea flour	30:58	Gluten-free biscuits based on composite rice-chickpea flour and xanthan gum.
29808729	3	46	theme	flour	379:383	arg1	%					374:374	0.5, 1, and 1.5%	359:374	0.5, 1, and 1.5% of flour	359:383	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	46	theme	flour	379:383	arg1	flour					379:383	flour	379:383	flour	379:383	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	2	47	theme	Xanthan	173:179	arg1	gum					181:183	Xanthan gum	173:183	Xanthan gum	173:183	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	3	48	theme	rheological	405:415	arg1	properties					417:426	rheological properties	405:426	rheological properties	405:426	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	0	49	theme	xanthan	64:70	arg1	gum					72:74	xanthan gum	64:74	xanthan gum	64:74	Gluten-free biscuits based on composite rice-chickpea flour and xanthan gum.
29808729	8	50	theme	biscuit	1256:1262	arg1	acceptability					1264:1276	rice-chickpea flour biscuit acceptability	1236:1276	rice-chickpea flour biscuit acceptability	1236:1276	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	4	51	theme	textural	643:650	arg1	properties					676:685	the textural and linear viscoelastic properties	639:685	the textural and linear viscoelastic properties of the dough	639:698	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	3	52	dep	incorporation	344:356	arg1	%					374:374	0.5, 1, and 1.5%	359:374	0.5, 1, and 1.5% of flour	359:383	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	52	dep	incorporation	344:356	arg1	flour					379:383	flour	379:383	flour	379:383	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	2	53	theme	handling	210:217	arg1	difficulties					219:230	the handling difficulties	206:230	the handling difficulties associated with the absence of gluten in the rice-chickpea flour	206:295	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	6	54	theme	specific	1014:1021	arg1	volume					1023:1028	specific volume	1014:1028	specific volume	1014:1028	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	9	55	theme	gluten-free	1451:1461	arg1	biscuits					1483:1490	gluten-free rice-chickpea flour biscuits	1451:1490	gluten-free rice-chickpea flour biscuits	1451:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	3	56	theme	biscuit	537:543	arg1	texture					452:458	the texture	448:458	the texture	448:458	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	56	theme	biscuit	537:543	arg1	acceptability					516:528	sensory acceptability	508:528	sensory acceptability	508:528	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	56	theme	biscuit	537:543	arg1	activity					489:496	water activity	483:496	water activity (aw)	483:501	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	56	theme	biscuit	537:543	arg1	dimensions					461:470	dimensions	461:470	dimensions	461:470	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	56	theme	biscuit	537:543	arg1	aw					499:500	aw	499:500	aw	499:500	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	56	theme	biscuit	537:543	arg1	moisture					473:480	moisture	473:480	moisture	473:480	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	2	57	theme	gluten	263:268	arg1	absence					252:258	the absence	248:258	the absence of gluten in the rice-chickpea flour	248:295	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	4	58	theme	linear	656:661	arg1	properties					676:685	the textural and linear viscoelastic properties	639:685	the textural and linear viscoelastic properties of the dough	639:698	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
29808729	9	59	theme	flour	1477:1481	arg1	biscuits					1483:1490	gluten-free rice-chickpea flour biscuits	1451:1490	gluten-free rice-chickpea flour biscuits	1451:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	6	60	dep	thickness	1000:1008	arg1	the					996:998	the	996:998	the	996:998	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	6	61	from	improvement	981:991	arg1	thickness					1000:1008	thickness	1000:1008	thickness	1000:1008	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	6	61	from	improvement	981:991	arg1	volume					1023:1028	specific volume	1014:1028	specific volume	1014:1028	The addition of xanthan gum resulted in a significant improvement in the thickness and specific volume of biscuits.
29808729	1	62	theme	composite	128:136	arg1	flour					152:156	composite rice-chickpea flour	128:156	composite rice-chickpea flour	128:156	A gluten-free biscuit for celiac children based on composite rice-chickpea flour was developed.
29808729	5	63	dep	hardness	826:833	arg1	the					822:824	the	822:824	the	822:824	Increasing the xanthan gum level increases the hardness and elasticity of the dough, and decreases its springiness, cohesiveness, and adhesiveness.
29808729	8	64	theme	sensory	1184:1190	arg1	acceptability					1192:1204	sensory acceptability	1184:1204	sensory acceptability	1184:1204	In terms of sensory acceptability, xanthan gum slightly reduces rice-chickpea flour biscuit acceptability, but the differences were not significant.
29808729	2	65	used	used	189:192	arg2	gum					181:183	Xanthan gum	173:183	Xanthan gum	173:183	Xanthan gum was used to overcome the handling difficulties associated with the absence of gluten in the rice-chickpea flour.
29808729	9	66	theme	technological	1375:1387	arg1	properties					1389:1398	the proper technological properties	1364:1398	the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits	1364:1490	Consequently, using xanthan gum to achieve the proper technological properties of dough required for the industrial production of gluten-free rice-chickpea flour biscuits is a viable alternative.
29808729	0	67	dep	biscuits	12:19	arg1	based					21:25	based	21:25	biscuits based on composite rice-chickpea flour and xanthan gum	12:74	Gluten-free biscuits based on composite rice-chickpea flour and xanthan gum.
29808729	1	68	theme	rice-chickpea	138:150	arg1	flour					152:156	composite rice-chickpea flour	128:156	composite rice-chickpea flour	128:156	A gluten-free biscuit for celiac children based on composite rice-chickpea flour was developed.
29808729	3	69	from	effect	302:307	arg1	texture					452:458	the texture	448:458	the texture	448:458	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	acceptability					516:528	sensory acceptability	508:528	sensory acceptability	508:528	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	activity					489:496	water activity	483:496	water activity (aw)	483:501	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	dimensions					461:470	dimensions	461:470	dimensions	461:470	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	texture					393:399	the texture	389:399	the texture	389:399	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	aw					499:500	aw	499:500	aw	499:500	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	69	from	effect	302:307	arg1	moisture					473:480	moisture	473:480	moisture	473:480	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	3	70	theme	xanthan	332:338	arg1	incorporation					344:356	xanthan gum incorporation	332:356	xanthan gum incorporation (0.5, 1, and 1.5% of flour)	332:384	The effect of different levels of xanthan gum incorporation (0.5, 1, and 1.5% of flour) on the texture and rheological properties of the dough and on the texture, dimensions, moisture, water activity (aw), and sensory acceptability of the biscuit was studied.
29808729	4	71	theme	rice-chickpea	596:608	arg1	flour					610:614	rice-chickpea flour	596:614	rice-chickpea flour	596:614	The incorporation of xanthan gum into rice-chickpea flour significantly affected the textural and linear viscoelastic properties of the dough, as well as the texture, weight, moisture, aw, and dimensions of the biscuits.
31600559	9	0	theme	galacturonic	1360:1371	arg1	acid					1373:1376	galacturonic acid	1360:1376	galacturonic acid	1360:1376	AAP01-1 was composed of seven monosaccharides, including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose.
31600559	7	1	theme	anticomplement	1041:1054	arg1	activities					1056:1065	Their anticomplement activities	1035:1065	Their anticomplement activities	1035:1065	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	16	2	theme	bioactive	2380:2388	arg1	constituents					2390:2401	bioactive constituents	2380:2401	bioactive constituents conferring heat-clearing effects of A. annua	2380:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	2	3	theme	immunoregulatory	337:352	arg1	activities					354:363	its anti-inflammatory and immunoregulatory activities	311:363	its anti-inflammatory and immunoregulatory activities	311:363	It has gained increasing attention for its anti-inflammatory and immunoregulatory activities.
31600559	8	4	theme	homogeneous	1179:1189	arg1	AAP01-1					1208:1214	AAP01-1	1208:1214	AAP01-1	1208:1214	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	4	theme	homogeneous	1179:1189	arg1	polysaccharides					1191:1205	Three homogeneous polysaccharides	1173:1205	Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3)	1173:1236	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	4	theme	homogeneous	1179:1189	arg1	AAP01-3					1229:1235	AAP01-3	1229:1235	AAP01-3	1229:1235	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	4	theme	homogeneous	1179:1189	arg1	AAP01-2					1217:1223	AAP01-2	1217:1223	AAP01-2	1217:1223	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	5	5	dep	MATERIALS	676:684	arg1	polysaccharides					718:732	Water-soluble crude polysaccharides	698:732	Water-soluble crude polysaccharides	698:732	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	16	6	theme	anticomplement	2329:2342	arg1	kind					2372:2375	another kind	2364:2375	another kind of bioactive constituents conferring heat-clearing effects of A. annua	2364:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	16	6	theme	anticomplement	2329:2342	arg1	polysaccharides					2344:2358	The anticomplement polysaccharides	2325:2358	The anticomplement polysaccharides	2325:2358	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	6	7	theme	NMR	944:946	arg1	analysis					948:955	NMR analysis	944:955	NMR analysis	944:955	Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides.
31600559	2	8	theme	anti-inflammatory	315:331	arg1	activities					354:363	its anti-inflammatory and immunoregulatory activities	311:363	its anti-inflammatory and immunoregulatory activities	311:363	It has gained increasing attention for its anti-inflammatory and immunoregulatory activities.
31600559	13	9	dep	CH50	1907:1910	arg1	AP50					1934:1937	AP50	1934:1937	AP50	1934:1937	AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL), while AAP01-1 was inactive.
31600559	13	9	dep	CH50	1907:1910	arg1	1.120 ± 0.052 mg/mL					1913:1931	1.120 ± 0.052 mg/mL	1913:1931	1.120 ± 0.052 mg/mL	1913:1931	AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL), while AAP01-1 was inactive.
31600559	13	9	dep	CH50	1907:1910	arg1	1.283 ± 0.061 mg/mL					1940:1958	1.283 ± 0.061 mg/mL	1940:1958	1.283 ± 0.061 mg/mL	1940:1958	AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL), while AAP01-1 was inactive.
31600559	11	10	dep	heteropolysaccharides	1544:1564	arg1	%					1618:1618	28%	1616:1618	28%	1616:1618	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	11	10	dep	heteropolysaccharides	1544:1564	arg1	%					1626:1626	15%	1624:1626	15%	1624:1626	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	11	10	dep	heteropolysaccharides	1544:1564	arg1	AAP01-3					1653:1659	AAP01-3	1653:1659	AAP01-3	1653:1659	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	11	10	dep	heteropolysaccharides	1544:1564	arg1	AAP01-2					1641:1647	AAP01-2	1641:1647	AAP01-2	1641:1647	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	11	10	dep	heteropolysaccharides	1544:1564	arg1	%					1613:1613	8%	1612:1613	8%	1612:1613	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	5	11	theme	annua	762:766	arg1	parts					750:754	the aerial parts	739:754	the aerial parts of A. annua	739:766	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	5	12	theme	Water-soluble	698:710	arg1	polysaccharides					718:732	Water-soluble crude polysaccharides	698:732	Water-soluble crude polysaccharides	698:732	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	15	13	from	activities	2276:2285	arg1	annua					2318:2322	annua	2318:2322	annua	2318:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	5	14	theme	gel	838:840	arg1	chromatography					853:866	DEAE-cellulose and Sephacryl S-300 gel permeation chromatography	803:866	chromatography	853:866	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	10	15	theme	acid	1508:1511	arg1	absence					1486:1492	the absence	1482:1492	the absence of glucuronic acid	1482:1511	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	11	16	theme	acidic	1537:1542	arg1	heteropolysaccharides					1544:1564	all branched acidic heteropolysaccharides	1524:1564	all branched acidic heteropolysaccharides	1524:1564	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	5	17	theme	DEAE-cellulose	803:816	arg1	chromatography					853:866	DEAE-cellulose and Sephacryl S-300 gel permeation chromatography	803:866	chromatography	853:866	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	10	18	contain	had	1433:1435	arg2	monosaccharides					1445:1459	similar monosaccharides	1437:1459	similar monosaccharides	1437:1459	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	10	18	contain	had	1433:1435	arg1	AAP01-3					1425:1431	AAP01-3	1425:1431	AAP01-3	1425:1431	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	10	18	contain	had	1433:1435	arg1	AAP01-2					1413:1419	AAP01-2	1413:1419	AAP01-2	1413:1419	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	15	19	from	annua	2318:2322	arg1	activities					2276:2285	anticomplement activities	2261:2285	anticomplement activities of the polysaccharides from A. annua	2261:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	15	19	from	annua	2318:2322	arg1	polysaccharides					2294:2308	the polysaccharides	2290:2308	the polysaccharides from A. annua	2290:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	5	20	theme	Sephacryl	822:830	arg1	chromatography					853:866	DEAE-cellulose and Sephacryl S-300 gel permeation chromatography	803:866	chromatography	853:866	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	4	21	theme	anticomplement	508:521	arg1	polysaccharides					535:549	its anticomplement homogeneous polysaccharides	504:549	its anticomplement homogeneous polysaccharides from A. annua	504:563	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	0	22	theme	homogeneous	74:84	arg1	polysaccharides					86:100	three acidic homogeneous polysaccharides	61:100	three acidic homogeneous polysaccharides from Artemisia annua	61:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	1	23	theme	heat-clearing	179:191	arg1	RELEVANCE					145:153	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	1	23	theme	heat-clearing	179:191	arg1	medicine					201:208	a heat-clearing Chinese medicine	177:208	a heat-clearing Chinese medicine	177:208	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	12	24	theme	CH50	1728:1731	arg1	value					1733:1737	CH50 value	1728:1737	CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway	1728:1790	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	3	25	theme	crude	385:389	arg1	polysaccahrides					391:405	the crude polysaccahrides	381:405	the crude polysaccahrides of A. annua	381:417	Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity.
31600559	3	26	theme	potent	429:434	arg1	activity					451:458	potent anticomplement activity	429:458	potent anticomplement activity	429:458	Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity.
31600559	15	27	theme	galacturonic	2224:2235	arg1	acid					2237:2240	galacturonic acid	2224:2240	galacturonic acid	2224:2240	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	12	28	theme	alternative	1842:1852	arg1	pathway					1854:1860	the alternative pathway	1838:1860	the alternative pathway	1838:1860	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	12	29	theme	potent	1692:1697	arg1	activity					1714:1721	potent anticomplement activity	1692:1721	potent anticomplement activity	1692:1721	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	3	30	theme	annua	413:417	arg1	polysaccahrides					391:405	the crude polysaccahrides	381:405	the crude polysaccahrides of A. annua	381:417	Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity.
31600559	11	31	with	heteropolysaccharides	1544:1564	arg1	contents					1581:1588	different contents	1571:1588	different contents of galacturonic acid	1571:1609	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	16	32	theme	heat-clearing	2414:2426	arg1	effects					2428:2434	heat-clearing effects	2414:2434	heat-clearing effects of A. annua	2414:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	0	33	from	activities	47:56	arg1	annua					117:121	Artemisia annua	107:121	Artemisia annua	107:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	7	34	from	components	1081:1090	arg1	cascade					1121:1127	the complement activation cascade	1095:1127	the complement activation cascade	1095:1127	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	16	35	theme	A.	2439:2440	arg1	annua					2442:2446	A. annua	2439:2446	A. annua	2439:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	0	36	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	0	37	from	annua	117:121	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	0	37	from	annua	117:121	arg1	activities					47:56	anticomplement activities	32:56	anticomplement activities	32:56	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	0	37	from	annua	117:121	arg1	polysaccharides					86:100	three acidic homogeneous polysaccharides	61:100	three acidic homogeneous polysaccharides from Artemisia annua	61:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	7	38	theme	activation	1110:1119	arg1	cascade					1121:1127	the complement activation cascade	1095:1127	the complement activation cascade	1095:1127	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	14	39	theme	complement	2133:2142	arg1	system					2144:2149	complement system	2133:2149	complement system	2133:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	4	40	theme	isolated	650:657	arg1	polysaccharides					659:673	the isolated polysaccharides	646:673	the isolated polysaccharides	646:673	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	10	41	theme	glucuronic	1497:1506	arg1	acid					1508:1511	glucuronic acid	1497:1511	glucuronic acid	1497:1511	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	5	42	from	parts	750:754	arg1	METHODS					690:696	METHODS	690:696	METHODS	690:696	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	5	42	from	parts	750:754	arg1	MATERIALS					676:684	MATERIALS	676:684	MATERIALS	676:684	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	8	43	dep	RESULTS	1165:1171	arg1	obtained					1243:1250	obtained	1243:1250	were obtained from A. annua	1238:1264	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	6	44	theme	polysaccharides	1018:1032	arg1	structures					992:1001	the structures	988:1001	the structures of homogeneous polysaccharides	988:1032	Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides.
31600559	4	45	theme	anticomplement	617:630	arg1	activities					632:641	anticomplement activities	617:641	anticomplement activities	617:641	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	15	46	dep	CONCLUSION	2152:2161	arg1	indicated					2177:2185	indicated	2177:2185	indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua	2177:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	6	47	theme	monosaccharide	900:913	arg1	composition					915:925	monosaccharide composition	900:925	monosaccharide composition	900:925	Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides.
31600559	7	48	theme	complement	1099:1108	arg1	cascade					1121:1127	the complement activation cascade	1095:1127	the complement activation cascade	1095:1127	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	9	49	theme	glucuronic	1343:1352	arg1	acid					1354:1357	glucuronic acid	1343:1357	glucuronic acid	1343:1357	AAP01-1 was composed of seven monosaccharides, including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose.
31600559	14	50	dep	C4	2026:2027	arg1	components					2040:2049	components	2040:2049	components	2040:2049	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	11	51	theme	different	1571:1579	arg1	contents					1581:1588	different contents	1571:1588	different contents of galacturonic acid	1571:1609	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	7	52	theme	targeting	1071:1079	arg1	components					1081:1090	targeting components	1071:1090	targeting components	1071:1090	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	16	53	theme	constituents	2390:2401	arg1	kind					2372:2375	another kind	2364:2375	another kind of bioactive constituents conferring heat-clearing effects of A. annua	2364:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	16	53	theme	constituents	2390:2401	arg1	polysaccharides					2344:2358	The anticomplement polysaccharides	2325:2358	The anticomplement polysaccharides	2325:2358	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	11	54	theme	acid	1606:1609	arg1	contents					1581:1588	different contents	1571:1588	different contents of galacturonic acid	1571:1609	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	14	55	theme	C3	2079:2080	arg1	components					2093:2102	C3, C4 and C5 components	2079:2102	C3, C4 and C5 components in the activation cascade of complement system	2079:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	5	56	theme	A.	759:760	arg1	annua					762:766	A. annua	759:766	A. annua	759:766	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	4	57	from	annua	559:563	arg1	polysaccharides					535:549	its anticomplement homogeneous polysaccharides	504:549	its anticomplement homogeneous polysaccharides from A. annua	504:563	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	1	58	theme	antimalarial	233:244	arg1	constituent					246:256	its antimalarial constituent	229:256	its antimalarial constituent	229:256	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	1	58	theme	antimalarial	233:244	arg1	artemisinin					259:269	artemisinin	259:269	artemisinin	259:269	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	12	59	theme	AP50	1796:1799	arg1	value					1801:1805	AP50 value	1796:1805	AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway	1796:1860	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	7	60	theme	hemolytic	1147:1155	arg1	assays					1157:1162	hemolytic assays	1147:1162	hemolytic assays	1147:1162	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	5	61	theme	permeation	842:851	arg1	chromatography					853:866	DEAE-cellulose and Sephacryl S-300 gel permeation chromatography	803:866	chromatography	853:866	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	15	62	theme	polysaccharides	2294:2308	arg1	activities					2276:2285	anticomplement activities	2261:2285	anticomplement activities of the polysaccharides from A. annua	2261:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	12	63	theme	0.360 ± 0.020 mg/mL	1742:1760	arg1	value					1801:1805	AP50 value	1796:1805	AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway	1796:1860	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	12	63	theme	0.360 ± 0.020 mg/mL	1742:1760	arg1	value					1733:1737	CH50 value	1728:1737	CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway	1728:1790	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	11	64	theme	branched	1528:1535	arg1	heteropolysaccharides					1544:1564	all branched acidic heteropolysaccharides	1524:1564	all branched acidic heteropolysaccharides	1524:1564	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
31600559	12	65	theme	classical	1774:1782	arg1	pathway					1784:1790	the classical pathway	1770:1790	the classical pathway	1770:1790	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	14	66	from	components	2093:2102	arg1	cascade					2122:2128	the activation cascade	2107:2128	the activation cascade of complement system	2107:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	5	67	theme	S-300	832:836	arg1	chromatography					853:866	DEAE-cellulose and Sephacryl S-300 gel permeation chromatography	803:866	chromatography	853:866	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	4	68	theme	homogeneous	523:533	arg1	polysaccharides					535:549	its anticomplement homogeneous polysaccharides	504:549	its anticomplement homogeneous polysaccharides from A. annua	504:563	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	0	69	theme	polysaccharides	86:100	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	0	69	theme	polysaccharides	86:100	arg1	activities					47:56	anticomplement activities	32:56	anticomplement activities	32:56	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	3	70	theme	A.	410:411	arg1	annua					413:417	A. annua	410:417	A. annua	410:417	Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity.
31600559	1	71	dep	RELEVANCE	145:153	arg1	L.					171:172	L.	171:172	L.	171:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	0	72	theme	Artemisia	107:115	arg1	annua					117:121	Artemisia annua	107:121	Artemisia annua	107:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	1	73	theme	Chinese	193:199	arg1	RELEVANCE					145:153	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	1	73	theme	Chinese	193:199	arg1	medicine					201:208	a heat-clearing Chinese medicine	177:208	a heat-clearing Chinese medicine	177:208	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	0	74	from	characterization	11:26	arg1	annua					117:121	Artemisia annua	107:121	Artemisia annua	107:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	10	75	theme	similar	1437:1443	arg1	monosaccharides					1445:1459	similar monosaccharides	1437:1459	similar monosaccharides	1437:1459	AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid.
31600559	12	76	theme	anticomplement	1699:1712	arg1	activity					1714:1721	potent anticomplement activity	1692:1721	potent anticomplement activity	1692:1721	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	3	77	theme	anticomplement	436:449	arg1	activity					451:458	potent anticomplement activity	429:458	potent anticomplement activity	429:458	Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity.
31600559	7	78	from	activities	1056:1065	arg1	cascade					1121:1127	the complement activation cascade	1095:1127	the complement activation cascade	1095:1127	Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays.
31600559	5	79	theme	aerial	743:748	arg1	parts					750:754	the aerial parts	739:754	the aerial parts of A. annua	739:766	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	15	80	theme	high	2207:2210	arg1	important					2247:2255	important	2247:2255	important	2247:2255	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	15	80	theme	high	2207:2210	arg1	contents					2212:2219	the relatively high contents	2192:2219	the relatively high contents of galacturonic acid	2192:2240	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	16	81	theme	annua	2442:2446	arg1	effects					2428:2434	heat-clearing effects	2414:2434	heat-clearing effects of A. annua	2414:2446	The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.
31600559	14	82	theme	system	2144:2149	arg1	cascade					2122:2128	the activation cascade	2107:2128	the activation cascade of complement system	2107:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	4	83	theme	polysaccharides	659:673	arg1	structures					602:611	structures	602:611	structures	602:611	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	4	83	theme	polysaccharides	659:673	arg1	activities					632:641	anticomplement activities	617:641	anticomplement activities	617:641	This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides.
31600559	13	84	theme	weaker	1890:1895	arg1	CH50					1907:1910	CH50	1907:1910	CH50	1907:1910	AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL), while AAP01-1 was inactive.
31600559	13	84	theme	weaker	1890:1895	arg1	activity					1897:1904	slightly weaker activity	1881:1904	slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL)	1881:1959	AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ± 0.052 mg/mL, AP50: 1.283 ± 0.061 mg/mL), while AAP01-1 was inactive.
31600559	8	85	dep	polysaccharides	1191:1205	arg1	AAP01-1					1208:1214	AAP01-1	1208:1214	AAP01-1	1208:1214	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	85	dep	polysaccharides	1191:1205	arg1	polysaccharides					1191:1205	Three homogeneous polysaccharides	1173:1205	Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3)	1173:1236	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	85	dep	polysaccharides	1191:1205	arg1	AAP01-3					1229:1235	AAP01-3	1229:1235	AAP01-3	1229:1235	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	8	85	dep	polysaccharides	1191:1205	arg1	AAP01-2					1217:1223	AAP01-2	1217:1223	AAP01-2	1217:1223	RESULTS Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua.
31600559	0	86	theme	anticomplement	32:45	arg1	activities					47:56	anticomplement activities	32:56	anticomplement activities	32:56	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	14	87	theme	C4	2083:2084	arg1	components					2093:2102	C3, C4 and C5 components	2079:2102	C3, C4 and C5 components in the activation cascade of complement system	2079:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	5	88	theme	crude	712:716	arg1	polysaccharides					718:732	Water-soluble crude polysaccharides	698:732	Water-soluble crude polysaccharides	698:732	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography.
31600559	1	89	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	medicine					201:208	a heat-clearing Chinese medicine	177:208	a heat-clearing Chinese medicine	177:208	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	1	89	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	RELEVANCE					145:153	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L.	124:172	ETHNOPHARMACOLOGICAL RELEVANCE Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin.
31600559	14	90	theme	activation	2111:2120	arg1	cascade					2122:2128	the activation cascade	2107:2128	the activation cascade of complement system	2107:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	12	91	theme	0.547 ± 0.033 mg/mL	1810:1828	arg1	value					1801:1805	AP50 value	1796:1805	AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway	1796:1860	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	12	91	theme	0.547 ± 0.033 mg/mL	1810:1828	arg1	value					1733:1737	CH50 value	1728:1737	CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway	1728:1790	AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ± 0.020 mg/mL through the classical pathway and AP50 value of 0.547 ± 0.033 mg/mL through the alternative pathway.
31600559	0	92	theme	acidic	67:72	arg1	polysaccharides					86:100	three acidic homogeneous polysaccharides	61:100	three acidic homogeneous polysaccharides from Artemisia annua	61:121	Structural characterization and anticomplement activities of three acidic homogeneous polysaccharides from Artemisia annua.
31600559	6	93	theme	homogeneous	1006:1016	arg1	polysaccharides					1018:1032	homogeneous polysaccharides	1006:1032	homogeneous polysaccharides	1006:1032	Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides.
31600559	15	94	theme	anticomplement	2261:2274	arg1	activities					2276:2285	anticomplement activities	2261:2285	anticomplement activities of the polysaccharides from A. annua	2261:2322	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	14	95	theme	C5	2090:2091	arg1	components					2093:2102	C3, C4 and C5 components	2079:2102	C3, C4 and C5 components in the activation cascade of complement system	2079:2149	Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system.
31600559	6	96	theme	molecular	882:890	arg1	weight					892:897	molecular weight	882:897	molecular weight	882:897	Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides.
31600559	15	97	theme	acid	2237:2240	arg1	important					2247:2255	important	2247:2255	important	2247:2255	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	15	97	theme	acid	2237:2240	arg1	contents					2212:2219	the relatively high contents	2192:2219	the relatively high contents of galacturonic acid	2192:2240	CONCLUSION These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua.
31600559	11	98	theme	galacturonic	1593:1604	arg1	acid					1606:1609	galacturonic acid	1593:1609	galacturonic acid	1593:1609	They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively).
30301347	4	0	dep	%	707:707	arg1	20					705:706	20	705:706	20	705:706	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	4	0	dep	%	707:707	arg1	w/w					710:712	w/w	710:712	w/w	710:712	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	4	0	dep	%	707:707	arg1	5					699:699	5	699:699	5	699:699	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	5	1	theme	PBAT	854:857	arg1	ductility					841:849	severely impaired the ductility	819:849	severely impaired the ductility of PBAT	819:857	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	5	1	theme	PBAT	854:857	arg1	aggregation					773:783	aggregation	773:783	aggregation	773:783	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	5	1	theme	PBAT	854:857	arg1	adhesion					791:798	poor adhesion	786:798	poor adhesion in the matrix	786:812	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	4	2	theme	reinforcing	616:626	arg1	effect					628:633	a clear reinforcing effect	608:633	a clear reinforcing effect	608:633	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	6	3	theme	5	1064:1064	arg1	%					1065:1065	%	1065:1065	%	1065:1065	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	4	4	theme	clear	610:614	arg1	effect					628:633	a clear reinforcing effect	608:633	a clear reinforcing effect	608:633	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	3	5	from	PBAT	553:556	arg1	composites					561:570	composites	561:570	composites prepared by melt extrusion	561:597	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	6	theme	melt	584:587	arg1	extrusion					589:597	melt extrusion	584:597	melt extrusion	584:597	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	4	7	contain	had	604:606	arg1	CNF					600:602	CNF	600:602	CNF	600:602	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	4	7	contain	had	604:606	arg2	effect					628:633	a clear reinforcing effect	608:633	a clear reinforcing effect	608:633	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	5	8	from	ductility	841:849	arg1	matrix					807:812	the matrix	803:812	the matrix	803:812	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	6	9	theme	%	1065:1065	arg1	level					1082:1086	5% (w/w) addition level	1064:1086	5% (w/w) addition level	1064:1086	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	0	10	from	Integration	50:60	arg1	Nanocomposites					76:89	Degradable Nanocomposites	65:89	Degradable Nanocomposites	65:89	Admicellar Polymerization Coating of CNF Enhances Integration in Degradable Nanocomposites.
30301347	3	11	with	compatibility	453:465	arg1	matrix					503:508	a degradable plastic composite matrix	472:508	a degradable plastic composite matrix	472:508	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	12	theme	plastic	485:491	arg1	matrix					503:508	a degradable plastic composite matrix	472:508	a degradable plastic composite matrix	472:508	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	5	13	from	aggregation	773:783	arg1	matrix					807:812	the matrix	803:812	the matrix	803:812	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	6	14	theme	addition	1073:1080	arg1	level					1082:1086	5% (w/w) addition level	1064:1086	5% (w/w) addition level	1064:1086	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	4	15	from	content	719:725	arg1	%					685:685	at least 35%	674:685	at least 35%	674:685	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	4	15	from	content	719:725	arg1	%					694:694	169%	691:694	169% at 5 and 20% (w/w) CNF content	691:725	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	3	16	theme	composite	493:501	arg1	matrix					503:508	a degradable plastic composite matrix	472:508	a degradable plastic composite matrix	472:508	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	0	17	theme	Polymerization	11:24	arg1	Coating					26:32	Admicellar Polymerization Coating	0:32	Admicellar Polymerization Coating of CNF	0:39	Admicellar Polymerization Coating of CNF Enhances Integration in Degradable Nanocomposites.
30301347	2	18	theme	acrylate	339:346	arg1	polymers					348:355	fatty acrylate polymers	333:355	fatty acrylate polymers	333:355	CNF was chemically modified through admicellar polymerization, producing fibrils coated with fatty acrylate polymers.
30301347	1	19	theme	water-based	94:104	arg1	strategy					124:131	A water-based one-pot synthesis strategy	92:131	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade	92:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	5	20	from	adhesion	791:798	arg1	matrix					807:812	the matrix	803:812	the matrix	803:812	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	5	21	theme	impaired	828:835	arg1	ductility					841:849	severely impaired the ductility	819:849	severely impaired the ductility of PBAT	819:857	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	0	22	theme	Admicellar	0:9	arg1	Coating					26:32	Admicellar Polymerization Coating	0:32	Admicellar Polymerization Coating of CNF	0:39	Admicellar Polymerization Coating of CNF Enhances Integration in Degradable Nanocomposites.
30301347	1	23	theme	one-pot	106:112	arg1	strategy					124:131	A water-based one-pot synthesis strategy	92:131	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade	92:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	6	24	theme	better	973:978	arg1	preservation					980:991	significantly better preservation	959:991	significantly better preservation of the elongation properties	959:1020	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	3	25	theme	degradable	474:483	arg1	matrix					503:508	a degradable plastic composite matrix	472:508	a degradable plastic composite matrix	472:508	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	5	26	theme	poor	786:789	arg1	adhesion					791:798	poor adhesion	786:798	poor adhesion in the matrix	786:812	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
30301347	6	27	theme	PBT	937:939	arg1	matrix					941:946	the PBT matrix	933:946	the PBT matrix	933:946	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	2	28	theme	fatty	333:337	arg1	polymers					348:355	fatty acrylate polymers	333:355	fatty acrylate polymers	333:355	CNF was chemically modified through admicellar polymerization, producing fibrils coated with fatty acrylate polymers.
30301347	1	29	theme	biopolymer	211:220	arg1	grade					222:226	a hydrophobic and processable biopolymer grade	181:226	a hydrophobic and processable biopolymer grade	181:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	3	30	theme	interfibrillar	408:421	arg1	interactions					423:434	the interfibrillar interactions	404:434	the interfibrillar interactions	404:434	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	4	31	theme	CNF	715:717	arg1	content					719:725	5 and 20% (w/w) CNF content	699:725	5 and 20% (w/w) CNF content	699:725	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	6	32	dep	%	1065:1065	arg1	w/w					1068:1070	w/w	1068:1070	w/w	1068:1070	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	1	33	theme	processable	199:209	arg1	grade					222:226	a hydrophobic and processable biopolymer grade	181:226	a hydrophobic and processable biopolymer grade	181:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	0	34	theme	CNF	37:39	arg1	Coating					26:32	Admicellar Polymerization Coating	0:32	Admicellar Polymerization Coating of CNF	0:39	Admicellar Polymerization Coating of CNF Enhances Integration in Degradable Nanocomposites.
30301347	3	35	with	interactions	423:434	arg1	matrix					503:508	a degradable plastic composite matrix	472:508	a degradable plastic composite matrix	472:508	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	36	from	change	394:399	arg1	interactions					423:434	the interfibrillar interactions	404:434	the interfibrillar interactions	404:434	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	36	from	change	394:399	arg1	compatibility					453:465	improved CNF compatibility	440:465	improved CNF compatibility	440:465	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	6	37	theme	properties	1011:1020	arg1	preservation					980:991	significantly better preservation	959:991	significantly better preservation of the elongation properties	959:1020	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	3	38	theme	improved	440:447	arg1	compatibility					453:465	improved CNF compatibility	440:465	improved CNF compatibility	440:465	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	4	39	dep	content	719:725	arg1	%					707:707	%	707:707	%	707:707	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	3	40	theme	CNF	449:451	arg1	compatibility					453:465	improved CNF compatibility	440:465	improved CNF compatibility	440:465	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	1	41	theme	cellulose	148:156	arg1	CNF					171:173	CNF	171:173	CNF	171:173	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	1	41	theme	cellulose	148:156	arg1	nanofibrils					158:168	cellulose nanofibrils	148:168	cellulose nanofibrils (CNF)	148:174	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	1	42	theme	synthesis	114:122	arg1	strategy					124:131	A water-based one-pot synthesis strategy	92:131	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade	92:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	6	43	theme	elongation	1000:1009	arg1	properties					1011:1020	the elongation properties	996:1020	the elongation properties	996:1020	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	2	44	theme	admicellar	276:285	arg1	polymerization					287:300	admicellar polymerization	276:300	admicellar polymerization	276:300	CNF was chemically modified through admicellar polymerization, producing fibrils coated with fatty acrylate polymers.
30301347	3	45	theme	adipate-	525:532	arg1	co-terephthalate					534:549	butylene adipate- co-terephthalate	516:549	butylene adipate- co-terephthalate	516:549	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	45	theme	adipate-	525:532	arg1	poly					511:514	poly	511:514	poly(butylene adipate- co-terephthalate)	511:550	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	46	theme	butylene	516:523	arg1	co-terephthalate					534:549	butylene adipate- co-terephthalate	516:549	butylene adipate- co-terephthalate	516:549	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	46	theme	butylene	516:523	arg1	poly					511:514	poly	511:514	poly(butylene adipate- co-terephthalate)	511:550	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	3	47	theme	proposed	362:369	arg1	modification					371:382	The proposed modification	358:382	The proposed modification	358:382	The proposed modification targets a change in the interfibrillar interactions and improved CNF compatibility with a degradable plastic composite matrix, poly(butylene adipate- co-terephthalate), PBAT in composites prepared by melt extrusion.
30301347	6	48	theme	unmodified	1034:1043	arg1	CNF					1045:1047	unmodified CNF	1034:1047	unmodified CNF	1034:1047	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	1	49	theme	hydrophobic	183:193	arg1	grade					222:226	a hydrophobic and processable biopolymer grade	181:226	a hydrophobic and processable biopolymer grade	181:226	A water-based one-pot synthesis strategy for converting cellulose nanofibrils (CNF) into a hydrophobic and processable biopolymer grade is devised.
30301347	4	50	theme	Young	655:659	arg1	modulus					663:669	Young's modulus	655:669	Young's modulus	655:669	CNF had a clear reinforcing effect on PBAT, increasing Young's modulus by at least 35% and 169% at 5 and 20% (w/w) CNF content, respectively.
30301347	0	51	theme	Degradable	65:74	arg1	Nanocomposites					76:89	Degradable Nanocomposites	65:89	Degradable Nanocomposites	65:89	Admicellar Polymerization Coating of CNF Enhances Integration in Degradable Nanocomposites.
30301347	6	52	theme	admicellar	876:885	arg1	polymerization					887:900	admicellar polymerization	876:900	admicellar polymerization	876:900	CNF modified by admicellar polymerization was homogeneously dispersed in the PBT matrix and showed significantly better preservation of the elongation properties compared to unmodified CNF, especially at 5% (w/w) addition level.
30301347	5	53	theme	unmodified	751:760	arg1	CNF					762:764	unmodified CNF	751:764	unmodified CNF	751:764	However, unmodified CNF showed aggregation, poor adhesion in the matrix, and severely impaired the ductility of PBAT.
31726144	6	0	theme	specific	1054:1061	arg1	genera					1063:1068	some specific genera	1049:1068	some specific genera	1049:1068	Spearman's correlation showed that some specific genera, such as Odoribacter, AF12, and Rikenella, were strongly associated with obesity-related parameters.
31726144	7	1	theme	diet-induced	1270:1281	arg1	obesity					1283:1289	diet-induced obesity	1270:1289	diet-induced obesity	1270:1289	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	3	2	from	obesity	585:591	arg1	mice					596:599	mice	596:599	mice with the amelioration of dyslipidemia	596:637	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	2	3	theme	related	371:377	arg1	parameters					360:369	parameters	360:369	parameters related to obesity, gut microbiota composition, and the correlation between the parameters	360:460	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	7	4	theme	prebiotic	1233:1241	arg1	agent					1243:1247	a potential prebiotic agent	1221:1247	a potential prebiotic agent in the prevention of diet-induced obesity	1221:1289	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	7	4	theme	prebiotic	1233:1241	arg1	ASKP					1201:1204	ASKP	1201:1204	ASKP	1201:1204	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	2	5	theme	anti-obesogenic	320:334	arg1	effect					336:341	anti-obesogenic effect	320:341	anti-obesogenic effect of ASKP	320:349	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	5	6	theme	Proteobacteria	974:987	arg1	reduction					961:969	the reduction	957:969	the reduction of Proteobacteria, AF12, and Helicobacter	957:1011	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	7	7	theme	obesity	1283:1289	arg1	prevention					1256:1265	the prevention	1252:1265	the prevention of diet-induced obesity	1252:1289	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	5	8	theme	Helicobacter	1000:1011	arg1	reduction					961:969	the reduction	957:969	the reduction of Proteobacteria, AF12, and Helicobacter	957:1011	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	3	9	from	endotoxaemia	654:665	arg1	mice					596:599	mice	596:599	mice with the amelioration of dyslipidemia	596:637	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	5	10	theme	gut	909:911	arg1	dysbiosis					913:921	the gut dysbiosis	905:921	the gut dysbiosis induced by high-fat diet	905:946	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	5	11	theme	16S	861:863	arg1	rRNA					865:868	16S rRNA	861:868	16S rRNA analysis	861:877	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	2	12	theme	underlying	295:304	arg1	mechanisms					306:315	the underlying mechanisms	291:315	the underlying mechanisms on anti-obesogenic effect of ASKP in mice	291:357	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	1	13	theme	sphaerocephala	190:203	arg1	polysaccharide					212:225	Artemisia sphaerocephala Krasch polysaccharide	180:225	Artemisia sphaerocephala Krasch polysaccharide (ASKP)	180:232	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	1	13	theme	sphaerocephala	190:203	arg1	ASKP					228:231	ASKP	228:231	ASKP	228:231	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	3	14	with	mice	596:599	arg1	amelioration					610:621	the amelioration	606:621	the amelioration of dyslipidemia	606:637	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	5	15	theme	rRNA	865:868	arg1	analysis					870:877	16S rRNA analysis	861:877	16S rRNA analysis	861:877	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	1	16	theme	Krasch	205:210	arg1	polysaccharide					212:225	Artemisia sphaerocephala Krasch polysaccharide	180:225	Artemisia sphaerocephala Krasch polysaccharide (ASKP)	180:232	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	1	16	theme	Krasch	205:210	arg1	ASKP					228:231	ASKP	228:231	ASKP	228:231	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	0	17	theme	sphaerocephala	10:23	arg1	polysaccharide					32:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	2	18	theme	ASKP	346:349	arg1	effect					336:341	anti-obesogenic effect	320:341	anti-obesogenic effect of ASKP	320:349	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	3	19	theme	high-fat-diet-induced	563:583	arg1	obesity					585:591	high-fat-diet-induced obesity	563:591	high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia	563:637	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	2	20	theme	microbiota	395:404	arg1	composition					406:416	gut microbiota composition	391:416	gut microbiota composition	391:416	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	0	21	theme	Artemisia	0:8	arg1	polysaccharide					32:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	4	22	theme	genes	811:815	arg1	downregulation					785:798	the downregulation	781:798	the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ	781:858	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	2	23	theme	gut	391:393	arg1	composition					406:416	gut microbiota composition	391:416	gut microbiota composition	391:416	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	0	24	theme	microbiota	141:150	arg1	modulation					123:132	the modulation	119:132	the modulation of gut microbiota in diet-induced obese mice	119:177	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	5	25	theme	AF12	990:993	arg1	reduction					961:969	the reduction	957:969	the reduction of Proteobacteria, AF12, and Helicobacter	957:1011	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	0	26	theme	Krasch	25:30	arg1	polysaccharide					32:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide	0:45	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	0	27	theme	gut	137:139	arg1	microbiota					141:150	gut microbiota	137:150	gut microbiota	137:150	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	1	28	theme	Artemisia	180:188	arg1	polysaccharide					212:225	Artemisia sphaerocephala Krasch polysaccharide	180:225	Artemisia sphaerocephala Krasch polysaccharide (ASKP)	180:232	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	1	28	theme	Artemisia	180:188	arg1	ASKP					228:231	ASKP	228:231	ASKP	228:231	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	5	29	theme	high-fat	934:941	arg1	diet					943:946	high-fat diet	934:946	high-fat diet	934:946	16S rRNA analysis showed that ASKP mediated the gut dysbiosis induced by high-fat diet, such as the reduction of Proteobacteria, AF12, and Helicobacter.
31726144	0	30	theme	lipid	56:60	arg1	metabolism					62:71	lipid metabolism	56:71	lipid metabolism	56:71	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	0	31	theme	diet-induced	155:166	arg1	mice					174:177	diet-induced obese mice	155:177	diet-induced obese mice	155:177	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	7	32	theme	potential	1223:1231	arg1	agent					1243:1247	a potential prebiotic agent	1221:1247	a potential prebiotic agent in the prevention of diet-induced obesity	1221:1289	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	7	32	theme	potential	1223:1231	arg1	ASKP					1201:1204	ASKP	1201:1204	ASKP	1201:1204	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	4	33	theme	ASKP	721:724	arg1	administration					726:739	ASKP administration	721:739	ASKP administration	721:739	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	1	34	contain	have	253:256	arg1	polysaccharide					212:225	Artemisia sphaerocephala Krasch polysaccharide	180:225	Artemisia sphaerocephala Krasch polysaccharide (ASKP)	180:232	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	1	34	contain	have	253:256	arg2	bioactivities					263:275	many bioactivities	258:275	many bioactivities	258:275	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	1	34	contain	have	253:256	arg1	ASKP					228:231	ASKP	228:231	ASKP	228:231	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31726144	4	35	theme	genes	700:704	arg1	analyses					688:695	Relative expression analyses	668:695	Relative expression analyses of genes	668:704	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	7	36	from	agent	1243:1247	arg1	prevention					1256:1265	the prevention	1252:1265	the prevention of diet-induced obesity	1252:1289	Our results demonstrated that ASKP could serve as a potential prebiotic agent in the prevention of diet-induced obesity.
31726144	3	37	theme	dyslipidemia	626:637	arg1	amelioration					610:621	the amelioration	606:621	the amelioration of dyslipidemia	606:637	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	2	38	theme	bacterial	475:483	arg1	taxa					485:488	specific bacterial taxa	466:488	specific bacterial taxa	466:488	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	0	39	theme	obese	168:172	arg1	mice					174:177	diet-induced obese mice	155:177	diet-induced obese mice	155:177	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	0	40	from	modulation	123:132	arg1	mice					174:177	diet-induced obese mice	155:177	diet-induced obese mice	155:177	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	3	41	theme	metabolic	644:652	arg1	endotoxaemia					654:665	metabolic endotoxaemia	644:665	metabolic endotoxaemia	644:665	The results showed that ASKP significantly alleviated high-fat-diet-induced obesity in mice with the amelioration of dyslipidemia, and metabolic endotoxaemia.
31726144	4	42	theme	related	803:809	arg1	PPARγ					854:858	PPARγ	854:858	PPARγ	854:858	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	4	42	theme	related	803:809	arg1	FAS					835:837	FAS	835:837	FAS	835:837	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	4	42	theme	related	803:809	arg1	genes					811:815	related genes	803:815	related genes	803:815	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	4	42	theme	related	803:809	arg1	ACC-1					828:832	ACC-1	828:832	ACC-1	828:832	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	4	42	theme	related	803:809	arg1	SREBP-1c					840:847	SREBP-1c	840:847	SREBP-1c	840:847	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	4	43	theme	hepatic	751:757	arg1	metabolism					765:774	hepatic lipid metabolism	751:774	hepatic lipid metabolism	751:774	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	6	44	theme	obesity-related	1143:1157	arg1	parameters					1159:1168	obesity-related parameters	1143:1168	obesity-related parameters	1143:1168	Spearman's correlation showed that some specific genera, such as Odoribacter, AF12, and Rikenella, were strongly associated with obesity-related parameters.
31726144	4	45	theme	lipid	759:763	arg1	metabolism					765:774	hepatic lipid metabolism	751:774	hepatic lipid metabolism	751:774	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	2	46	from	mechanisms	306:315	arg1	effect					336:341	anti-obesogenic effect	320:341	anti-obesogenic effect of ASKP	320:349	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	2	46	from	mechanisms	306:315	arg1	mice					354:357	mice	354:357	mice	354:357	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	4	47	theme	expression	677:686	arg1	analyses					688:695	Relative expression analyses	668:695	Relative expression analyses of genes	668:704	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	0	48	theme	metabolic	77:85	arg1	endotoxaemia					87:98	metabolic endotoxaemia	77:98	metabolic endotoxaemia	77:98	Artemisia sphaerocephala Krasch polysaccharide mediates lipid metabolism and metabolic endotoxaemia in associated with the modulation of gut microbiota in diet-induced obese mice.
31726144	4	49	theme	Relative	668:675	arg1	analyses					688:695	Relative expression analyses	668:695	Relative expression analyses of genes	668:704	Relative expression analyses of genes indicated that ASKP administration modulated hepatic lipid metabolism with the downregulation of related genes, including ACC-1, FAS, SREBP-1c, and PPARγ.
31726144	2	50	theme	specific	466:473	arg1	taxa					485:488	specific bacterial taxa	466:488	specific bacterial taxa	466:488	To determine the underlying mechanisms on anti-obesogenic effect of ASKP in mice, parameters related to obesity, gut microbiota composition, and the correlation between the parameters and specific bacterial taxa were investigated.
31726144	1	51	theme	many	258:261	arg1	bioactivities					263:275	many bioactivities	258:275	many bioactivities	258:275	Artemisia sphaerocephala Krasch polysaccharide (ASKP) has been proved to have many bioactivities.
31820948	5	0	theme	biological	789:798	arg1	origins					800:806	the biological origins	785:806	the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	785:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	6	1	theme	independent	906:916	arg1	fact					901:904	fact	901:904	fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface	901:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	8	2	theme	nanocellulose	1325:1337	arg1	production					1340:1349	"nanocellulose" production	1324:1349	"nanocellulose" production	1324:1349	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	6	3	theme	conformation	970:981	arg1	independent					906:916	independent	906:916	independent	906:916	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	4	4	theme	volume	587:592	arg1	fraction					594:601	the volume fraction	583:601	the volume fraction of cellulose	583:614	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	5	5	theme	origins	800:806	arg1	irrespective					769:780	irrespective	769:780	irrespective	769:780	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	10	6	contain	has	1623:1625	arg1	density					1546:1552	The true density	1537:1552	The true density of nanocellulose	1537:1569	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	10	6	contain	has	1623:1625	arg2	gradient					1636:1643	a strong gradient	1627:1643	a strong gradient	1627:1643	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	1	7	from	quantity	176:183	arg1	science					97:103	science	97:103	science	97:103	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	7	from	quantity	176:183	arg1	materials					87:95	materials science and crystallography	87:123	materials science and crystallography	87:123	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	7	from	quantity	176:183	arg1	crystallography					109:123	crystallography	109:123	crystallography	109:123	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	4	8	theme	single	521:526	arg1	fibrils					528:534	the single fibrils	517:534	the single fibrils	517:534	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	7	9	theme	cellulose	1098:1106	arg1	molecules					1108:1116	all the cellulose molecules	1090:1116	all the cellulose molecules	1090:1116	In the single fibrils, all the cellulose molecules are densely packed from the crystalline core to the noncrystalline outermost regions.
31820948	8	10	theme	production	1340:1349	arg1	process					1313:1319	the wet disintegration process	1290:1319	the wet disintegration process of "nanocellulose" production	1290:1349	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	3	11	theme	single	381:386	arg1	fibrils					388:394	the single fibrils	377:394	the single fibrils	377:394	In the single fibrils, all the cellulose molecules are uniaxiallly oriented.
31820948	4	12	theme	molecular	488:496	arg1	density					506:512	the molecular packing density	484:512	the molecular packing density in the single fibrils	484:534	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	8	13	theme	"	1338:1338	arg1	production					1340:1349	"nanocellulose" production	1324:1349	"nanocellulose" production	1324:1349	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	1	14	dep	materials	87:95	arg1	science					97:103	science	97:103	science	97:103	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	14	dep	materials	87:95	arg1	materials					87:95	materials science and crystallography	87:123	materials science and crystallography	87:123	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	14	dep	materials	87:95	arg1	crystallography					109:123	crystallography	109:123	crystallography	109:123	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	7	15	theme	outermost	1185:1193	arg1	regions					1195:1201	the noncrystalline outermost regions	1166:1201	the noncrystalline outermost regions	1166:1201	In the single fibrils, all the cellulose molecules are densely packed from the crystalline core to the noncrystalline outermost regions.
31820948	4	16	from	density	506:512	arg1	fibrils					528:534	the single fibrils	517:534	the single fibrils	517:534	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	5	17	theme	crystallinity	863:875	arg1	origins					800:806	the biological origins	785:806	the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	785:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	1	18	theme	solids	188:193	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	18	theme	solids	188:193	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	4	19	theme	fraction	594:601	arg1	estimation					569:578	the precise estimation	557:578	the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures	557:662	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	6	20	theme	true	882:885	arg1	density					887:893	The true density	878:893	The true density	878:893	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	9	21	theme	fibrils	1408:1414	arg1	functionality					1387:1399	the surface functionality	1375:1399	the surface functionality of the fibrils by oxidation	1375:1427	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	2	22	theme	cellulose	291:299	arg1	crystallites					275:286	nanometer-wide fibrillar crystallites	250:286	nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	250:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	10	23	theme	true	1541:1544	arg1	density					1546:1552	The true density	1537:1552	The true density of nanocellulose	1537:1569	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	4	24	theme	packing	498:504	arg1	density					506:512	the molecular packing density	484:512	the molecular packing density in the single fibrils	484:534	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	6	25	theme	atomic	963:968	arg1	conformation					970:981	the atomic conformation	959:981	the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface	959:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	5	26	theme	cellulose	731:739	arg1	crystallites					715:726	fibrillar crystallites	705:726	fibrillar crystallites of cellulose	705:739	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	4	27	from	estimation	569:578	arg1	structures					653:662	porous structures	646:662	porous structures	646:662	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	4	27	from	estimation	569:578	arg1	composites					632:641	fibril-based composites	619:641	fibril-based composites	619:641	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	4	28	theme	precise	561:567	arg1	estimation					569:578	the precise estimation	557:578	the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures	557:662	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	6	29	from	surface	1058:1064	arg1	conformation					970:981	the atomic conformation	959:981	the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface	959:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	6	29	from	surface	1058:1064	arg1	dimension					925:933	the dimension	921:933	the dimension of the crystallites	921:953	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	5	30	dep	cellulose	815:823	arg1	tunicate					845:852	a tunicate	843:852	a tunicate	843:852	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	5	30	dep	cellulose	815:823	arg1	cotton					832:837	cotton	832:837	cotton	832:837	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	5	30	dep	cellulose	815:823	arg1	wood					826:829	wood	826:829	wood	826:829	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	0	31	theme	True	53:56	arg1	Density					58:64	Modification-Dependent True Density	30:64	Modification-Dependent True Density of Nanocellulose	30:81	Crystallinity-Independent yet Modification-Dependent True Density of Nanocellulose.
31820948	10	32	theme	fibril	1652:1657	arg1	direction					1675:1683	the fibril cross-sectional direction	1648:1683	the fibril cross-sectional direction	1648:1683	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	5	33	theme	crystallites	715:726	arg1	g/cm3					763:767	approximately 1.60 g/cm3	744:767	approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	744:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	5	33	theme	crystallites	715:726	arg1	density					694:700	the true density	685:700	the true density of fibrillar crystallites of cellulose	685:739	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	6	34	theme	oriented	1001:1008	arg1	molecules					1029:1037	the uniaxially oriented but noncrystalline molecules	986:1037	the uniaxially oriented but noncrystalline molecules	986:1037	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	4	35	theme	porous	646:651	arg1	structures					653:662	porous structures	646:662	porous structures	646:662	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	0	36	theme	Modification-Dependent	30:51	arg1	Density					58:64	Modification-Dependent True Density	30:64	Modification-Dependent True Density of Nanocellulose	30:81	Crystallinity-Independent yet Modification-Dependent True Density of Nanocellulose.
31820948	7	37	theme	single	1074:1079	arg1	fibrils					1081:1087	the single fibrils	1070:1087	the single fibrils	1070:1087	In the single fibrils, all the cellulose molecules are densely packed from the crystalline core to the noncrystalline outermost regions.
31820948	4	38	theme	true	461:464	arg1	essential					543:551	essential	543:551	essential	543:551	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	4	38	theme	true	461:464	arg1	density					466:472	the true density	457:472	the true density	457:472	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	1	39	theme	true	130:133	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	39	theme	true	130:133	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	6	40	theme	crystallite	1046:1056	arg1	surface					1058:1064	the crystallite surface	1042:1064	the crystallite surface	1042:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	6	41	theme	crystallites	942:953	arg1	conformation					970:981	the atomic conformation	959:981	the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface	959:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	6	41	theme	crystallites	942:953	arg1	dimension					925:933	the dimension	921:933	the dimension of the crystallites	921:953	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	1	42	link	derived	159:165	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	42	link	derived	159:165	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	7	43	theme	noncrystalline	1170:1183	arg1	regions					1195:1201	the noncrystalline outermost regions	1166:1201	the noncrystalline outermost regions	1166:1201	In the single fibrils, all the cellulose molecules are densely packed from the crystalline core to the noncrystalline outermost regions.
31820948	7	44	theme	crystalline	1146:1156	arg1	core					1158:1161	the crystalline core	1142:1161	the crystalline core to the noncrystalline outermost regions	1142:1201	In the single fibrils, all the cellulose molecules are densely packed from the crystalline core to the noncrystalline outermost regions.
31820948	10	45	theme	surface	1597:1603	arg1	functionality					1605:1617	the surface functionality	1593:1617	the surface functionality	1593:1617	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	2	46	theme	density	239:245	arg1	correlation					215:225	the correlation	211:225	the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	211:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	9	47	from	change	1472:1477	arg1	up					1499:1500	the true density up	1482:1500	the true density up to 1.8 g/cm3	1482:1513	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	9	47	from	change	1472:1477	arg1	down					1518:1521	down	1518:1521	down	1518:1521	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	5	48	theme	irrespective	769:780	arg1	g/cm3					763:767	approximately 1.60 g/cm3	744:767	approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	744:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	5	48	theme	irrespective	769:780	arg1	density					694:700	the true density	685:700	the true density of fibrillar crystallites of cellulose	685:739	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	3	49	theme	cellulose	405:413	arg1	molecules					415:423	all the cellulose molecules	397:423	all the cellulose molecules	397:423	In the single fibrils, all the cellulose molecules are uniaxiallly oriented.
31820948	5	50	theme	true	689:692	arg1	g/cm3					763:767	approximately 1.60 g/cm3	744:767	approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	744:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	5	50	theme	true	689:692	arg1	density					694:700	the true density	685:700	the true density of fibrillar crystallites of cellulose	685:739	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	9	51	theme	density	1491:1497	arg1	up					1499:1500	the true density up	1482:1500	the true density up to 1.8 g/cm3	1482:1513	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	2	52	theme	true	234:237	arg1	density					239:245	the true density	230:245	the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	230:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	9	53	theme	true	1486:1489	arg1	up					1499:1500	the true density up	1482:1500	the true density up to 1.8 g/cm3	1482:1513	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	0	54	theme	Nanocellulose	69:81	arg1	Density					58:64	Modification-Dependent True Density	30:64	Modification-Dependent True Density of Nanocellulose	30:81	Crystallinity-Independent yet Modification-Dependent True Density of Nanocellulose.
31820948	6	55	theme	dimension	925:933	arg1	independent					906:916	independent	906:916	independent	906:916	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	2	56	theme	surface	351:357	arg1	functionality					359:371	surface functionality	351:371	surface functionality	351:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	2	57	with	crystallites	275:286	arg1	crystallinity					320:332	crystallinity	320:332	crystallinity	320:332	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	2	57	with	crystallites	275:286	arg1	purity					312:317	purity	312:317	purity	312:317	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	2	57	with	crystallites	275:286	arg1	functionality					359:371	surface functionality	351:371	surface functionality	351:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	2	57	with	crystallites	275:286	arg1	morphology					335:344	morphology	335:344	morphology	335:344	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	10	58	theme	cross-sectional	1659:1673	arg1	direction					1675:1683	the fibril cross-sectional direction	1648:1683	the fibril cross-sectional direction	1648:1683	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	6	59	theme	noncrystalline	1014:1027	arg1	molecules					1029:1037	the uniaxially oriented but noncrystalline molecules	986:1037	the uniaxially oriented but noncrystalline molecules	986:1037	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	8	60	theme	disintegration	1298:1311	arg1	process					1313:1319	the wet disintegration process	1290:1319	the wet disintegration process of "nanocellulose" production	1290:1349	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	9	61	theme	surface	1379:1385	arg1	functionality					1387:1399	the surface functionality	1375:1399	the surface functionality of the fibrils by oxidation	1375:1427	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	6	62	theme	molecules	1029:1037	arg1	conformation					970:981	the atomic conformation	959:981	the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface	959:1064	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	6	62	theme	molecules	1029:1037	arg1	dimension					925:933	the dimension	921:933	the dimension of the crystallites	921:953	The true density is in fact independent of the dimension of the crystallites and the atomic conformation of the uniaxially oriented but noncrystalline molecules at the crystallite surface.
31820948	5	63	theme	fibrillar	705:713	arg1	crystallites					715:726	fibrillar crystallites	705:726	fibrillar crystallites of cellulose	705:739	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	2	64	theme	crystallites	275:286	arg1	density					239:245	the true density	230:245	the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	230:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	1	65	theme	important	149:157	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	65	theme	important	149:157	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	8	66	theme	g/cm3	1222:1226	arg1	value					1208:1212	The value	1204:1212	The value of 1.60 g/cm3	1204:1226	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	4	67	theme	cellulose	606:614	arg1	fraction					594:601	the volume fraction	583:601	the volume fraction of cellulose	583:614	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31820948	2	68	theme	fibrillar	265:273	arg1	crystallites					275:286	nanometer-wide fibrillar crystallites	250:286	nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	250:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	9	69	theme	substantial	1460:1470	arg1	change					1472:1477	a substantial change	1458:1477	a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3	1458:1534	In contrast, tailoring the surface functionality of the fibrils by oxidation and/or adsorption results in a substantial change in the true density up to 1.8 g/cm3 or down to 1.3 g/cm3.
31820948	1	70	theme	derived	159:165	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	70	theme	derived	159:165	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	8	71	theme	wet	1294:1296	arg1	process					1313:1319	the wet disintegration process	1290:1319	the wet disintegration process of "nanocellulose" production	1290:1349	The value of 1.60 g/cm3 remains unchanged even when the fibrils are dispersed through the wet disintegration process of "nanocellulose" production.
31820948	10	72	theme	strong	1629:1634	arg1	gradient					1636:1643	a strong gradient	1627:1643	a strong gradient	1627:1643	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	5	73	theme	cellulose	815:823	arg1	origins					800:806	the biological origins	785:806	the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity	785:875	We demonstrate that the true density of fibrillar crystallites of cellulose is approximately 1.60 g/cm3 irrespective of the biological origins of the cellulose (wood, cotton, or a tunicate) and the crystallinity.
31820948	2	74	theme	nanometer-wide	250:263	arg1	crystallites					275:286	nanometer-wide fibrillar crystallites	250:286	nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality	250:371	Here we report the correlation of the true density of nanometer-wide fibrillar crystallites of cellulose with their purity, crystallinity, morphology, and surface functionality.
31820948	10	75	theme	nanocellulose	1557:1569	arg1	density					1546:1552	The true density	1537:1552	The true density of nanocellulose	1537:1569	The true density of nanocellulose is indeed governed by the surface functionality and has a strong gradient in the fibril cross-sectional direction.
31820948	1	76	theme	physical	167:174	arg1	density					135:141	the true density	126:141	the true density	126:141	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	1	76	theme	physical	167:174	arg1	quantity					176:183	an important derived physical quantity	146:183	an important derived physical quantity of solids	146:193	In materials science and crystallography, the true density is an important derived physical quantity of solids.
31820948	4	77	theme	fibril-based	619:630	arg1	composites					632:641	fibril-based composites	619:641	fibril-based composites	619:641	Thus, the true density indicates the molecular packing density in the single fibrils and is essential for the precise estimation of the volume fraction of cellulose in fibril-based composites or porous structures.
31874683	1	0	theme	genus	162:166	arg1	Cellulosimicrobium					168:185	the genus Cellulosimicrobium	158:185	the genus Cellulosimicrobium	158:185	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	0	1	theme	Cellulosimicrobium	75:92	arg1	species					94:100	Cellulosimicrobium species	75:100	Cellulosimicrobium species	75:100	Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.
31874683	1	2	theme	Cellulosimicrobium	168:185	arg1	species					147:153	type species	142:153	type species of the genus Cellulosimicrobium	142:185	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	3	3	theme	proteomic	594:602	arg1	techniques					604:613	proteomic techniques	594:613	proteomic techniques	594:613	Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed.
31874683	3	4	theme	structure	539:547	arg1	identifications					549:563	subunit structure identifications	531:563	subunit structure identifications	531:563	Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed.
31874683	1	5	theme	water-insoluble	284:298	arg1	polysaccharides					300:314	water-insoluble polysaccharides	284:314	water-insoluble polysaccharides	284:314	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	2	6	theme	other	406:410	arg1	species					412:418	two other species	402:418	two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum)	402:505	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	5	7	theme	enzymatic	850:858	arg1	activities					860:869	different enzymatic activities	840:869	different enzymatic activities	840:869	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	4	8	theme	isolated	705:712	arg1	MECs					714:717	the isolated MECs	701:717	the isolated MECs	701:717	When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins.
31874683	4	9	theme	multidomain	785:795	arg1	proteins					797:804	at least three conserved multidomain proteins	760:804	at least three conserved multidomain proteins	760:804	When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins.
31874683	1	10	theme	xylanase	197:204	arg1	complexes					240:248	xylanase predominant nanoscale multienzyme complexes	197:248	xylanase predominant nanoscale multienzyme complexes	197:248	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	1	11	theme	Cellulosimicrobium	103:120	arg1	cellulans					122:130	Cellulosimicrobium cellulans	103:130	Cellulosimicrobium cellulans	103:130	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	1	12	theme	predominant	206:216	arg1	complexes					240:248	xylanase predominant nanoscale multienzyme complexes	197:248	xylanase predominant nanoscale multienzyme complexes	197:248	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	5	13	theme	subunit	875:881	arg1	compositions					883:894	subunit compositions	875:894	subunit compositions	875:894	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	4	14	theme	conserved	775:783	arg1	proteins					797:804	at least three conserved multidomain proteins	760:804	at least three conserved multidomain proteins	760:804	When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins.
31874683	1	15	theme	nanoscale	218:226	arg1	complexes					240:248	xylanase predominant nanoscale multienzyme complexes	197:248	xylanase predominant nanoscale multienzyme complexes	197:248	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	0	16	theme	subunit	14:20	arg1	compositions					22:33	subunit compositions	14:33	subunit compositions	14:33	Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.
31874683	1	17	dep	xylanosomes	257:267	arg1	i.e.					251:254	i.e.	251:254	i.e.	251:254	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	2	18	theme	complexes	373:381	arg1	isolation					340:348	the isolation	336:348	the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum)	336:505	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	1	19	theme	multienzyme	228:238	arg1	complexes					240:248	xylanase predominant nanoscale multienzyme complexes	197:248	xylanase predominant nanoscale multienzyme complexes	197:248	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	3	20	theme	subunit	531:537	arg1	identifications					549:563	subunit structure identifications	531:563	subunit structure identifications	531:563	Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed.
31874683	5	21	theme	diverse	913:919	arg1	capability					921:930	diverse capability	913:930	diverse capability	913:930	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	0	22	theme	complexes	53:61	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.
31874683	0	22	theme	complexes	53:61	arg1	compositions					22:33	subunit compositions	14:33	subunit compositions	14:33	Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.
31874683	5	23	theme	degrading	950:958	arg1	hemicelluloses					960:973	degrading hemicelluloses	950:973	degrading hemicelluloses	950:973	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	2	24	dep	Cellulosimicrobium	429:446	arg1	funkei					468:473	Cellulosimicrobium funkei	449:473	Cellulosimicrobium funkei	449:473	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	2	24	dep	Cellulosimicrobium	429:446	arg1	terreum					498:504	Cellulosimicrobium terreum	479:504	Cellulosimicrobium terreum	479:504	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	2	25	theme	multienzyme	361:371	arg1	MECs					384:387	MECs	384:387	MECs	384:387	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	2	25	theme	multienzyme	361:371	arg1	complexes					373:381	similar multienzyme complexes	353:381	similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum)	353:505	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	0	26	theme	xylanosome	42:51	arg1	complexes					53:61	the xylanosome complexes	38:61	the xylanosome complexes	38:61	Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.
31874683	2	27	theme	similar	353:359	arg1	MECs					384:387	MECs	384:387	MECs	384:387	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	2	27	theme	similar	353:359	arg1	complexes					373:381	similar multienzyme complexes	353:381	similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum)	353:505	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	1	28	theme	type	142:145	arg1	species					147:153	type species	142:153	type species of the genus Cellulosimicrobium	142:185	Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides.
31874683	5	29	from	strategies	936:945	arg1	hemicelluloses					960:973	degrading hemicelluloses	950:973	degrading hemicelluloses	950:973	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	4	30	theme	larger	728:733	arg1	size					744:747	a larger particle size	726:747	a larger particle size	726:747	When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins.
31874683	3	31	theme	Functional	508:517	arg1	studies					519:525	Functional studies	508:525	Functional studies	508:525	Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed.
31874683	2	32	from	species	412:418	arg1	Cellulosimicrobium					429:446	genus Cellulosimicrobium	423:446	genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum)	423:505	Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum).
31874683	4	33	theme	particle	735:742	arg1	size					744:747	a larger particle size	726:747	a larger particle size	726:747	When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins.
31874683	5	34	theme	different	840:848	arg1	activities					860:869	different enzymatic activities	840:869	different enzymatic activities	840:869	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	5	35	from	capability	921:930	arg1	hemicelluloses					960:973	degrading hemicelluloses	950:973	degrading hemicelluloses	950:973	In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
31874683	3	36	theme	genomic	571:577	arg1	sequencing					579:588	genomic sequencing	571:588	genomic sequencing	571:588	Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed.
31374273	2	0	dep	carriers	488:495	arg1	HLG					501:503	HLG	501:503	HLG	501:503	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	0	dep	carriers	488:495	arg1	LSM					497:499	LSM	497:499	LSM	497:499	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	1	theme	2.59 years	1349:1358	arg1	life					1335:1338	shelf life	1329:1338	shelf life of about 2.59 years	1329:1358	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	2	theme	7.4pH	1190:1194	arg1	media					1205:1209	7.4pH alkaline media	1190:1209	7.4pH alkaline media	1190:1209	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	3	dep	ex	1067:1068	arg1	vivo					1070:1073	vivo	1070:1073	vivo	1070:1073	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	2	4	from	carriers	488:495	arg1	solution					544:551	curing solution	537:551	curing solution	537:551	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	5	theme	polymer	518:524	arg1	pectin					526:531	enteric polymer pectin	510:531	enteric polymer pectin (in curing solution)	510:552	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	6	theme	shelf	1329:1333	arg1	life					1335:1338	shelf life	1329:1338	shelf life of about 2.59 years	1329:1358	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	4	7	dep	favorable	825:833	arg1	in					835:836	in	835:836	in	835:836	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	5	8	theme	1.2pH	1167:1171	arg1	media					1173:1177	1.2pH media	1167:1177	1.2pH media	1167:1177	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	9	theme	Hibiscus	170:177	arg1	gum					184:186	Hibiscus Leaf gum	170:186	Hibiscus Leaf gum(HLG)	170:191	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	9	theme	Hibiscus	170:177	arg1	HLG					188:190	HLG	188:190	HLG	188:190	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	10	theme	gum	184:186	arg1	blend					132:136	a blend	130:136	a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG)	130:191	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	10	theme	gum	184:186	arg1	carriers					209:216	mucoadhesive carriers	196:216	mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB)	196:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	4	11	theme	optimized	765:773	arg1	F18					779:781	F18	779:781	F18	779:781	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	4	11	theme	optimized	765:773	arg1	CMB					775:777	The optimized CMB	761:777	The optimized CMB(F18)	761:782	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	2	12	theme	drug	607:610	arg1	release					612:618	%drug release	606:618	%drug release (%DR)	606:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	12	theme	drug	607:610	arg1	DR					622:623	%DR	621:623	%DR	621:623	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	1	13	theme	CPTB	341:344	arg1	release					330:336	sustained release	320:336	sustained release of CPTB (BCS Class I drug) in the colon region	320:383	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	14	theme	carriers	488:495	arg1	quantities					461:470	quantities	461:470	quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution)	461:552	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	6	15	theme	mucoadhesive	1427:1438	arg1	carriers					1440:1447	ideal mucoadhesive carriers	1421:1447	ideal mucoadhesive carriers	1421:1447	Hence the study confirms that the combination of LSM&HLG as ideal mucoadhesive carriers and can favorably target highly soluble drugs to the colon region.
31374273	4	16	theme	non-Fickian	928:938	arg1	mechanism					950:958	zero order non-Fickian diffusion mechanism	917:958	zero order non-Fickian diffusion mechanism	917:958	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	1	17	theme	BCS	347:349	arg1	CPTB					341:344	CPTB	341:344	CPTB (BCS Class I drug)	341:363	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	17	theme	BCS	347:349	arg1	drug					359:362	BCS Class I drug	347:362	BCS Class I drug	347:362	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	3	18	theme	bead	681:684	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	18	theme	bead	681:684	arg1	efficiency					749:758	maximum drug encapsulation efficiency	722:758	maximum drug encapsulation efficiency	722:758	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	18	theme	bead	681:684	arg1	morphology					710:719	spherical surface morphology	692:719	spherical surface morphology	692:719	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	1	19	theme	I	357:357	arg1	CPTB					341:344	CPTB	341:344	CPTB (BCS Class I drug)	341:363	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	19	theme	I	357:357	arg1	drug					359:362	BCS Class I drug	347:362	BCS Class I drug	347:362	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	4	20	theme	maximum	794:800	arg1	loading					808:814	maximum %drug loading	794:814	maximum %drug loading(28.94%)	794:822	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	4	20	theme	maximum	794:800	arg1	%					821:821	28.94%	816:821	28.94%	816:821	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	5	21	theme	ex	1067:1068	arg1	study					1088:1092	ex vivo mucoadhesive study	1067:1092	ex vivo mucoadhesive study	1067:1092	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	4	22	from	release	849:855	arg1	12 h					876:879	12 h	876:879	12 h	876:879	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	3	23	theme	surface	702:708	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	23	theme	surface	702:708	arg1	morphology					710:719	spherical surface morphology	692:719	spherical surface morphology	692:719	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	4	24	theme	drug	803:806	arg1	loading					808:814	maximum %drug loading	794:814	maximum %drug loading(28.94%)	794:822	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	4	24	theme	drug	803:806	arg1	%					821:821	28.94%	816:821	28.94%	816:821	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	2	25	theme	drug	583:586	arg1	loading					588:594	%drug loading	582:594	%drug loading (%DL)	582:600	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	25	theme	drug	583:586	arg1	DL					598:599	%DL	597:599	%DL	597:599	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	26	theme	alkaline	1024:1031	arg1	media					1033:1037	alkaline media	1024:1037	alkaline media	1024:1037	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	3	27	theme	ion-gelation	643:654	arg1	technique					656:664	ion-gelation technique	643:664	ion-gelation technique	643:664	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	0	28	theme	colon	83:87	arg1	targeting					89:97	colon targeting	83:97	colon targeting	83:97	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	3	29	theme	drug	730:733	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	29	theme	drug	730:733	arg1	efficiency					749:758	maximum drug encapsulation efficiency	722:758	maximum drug encapsulation efficiency	722:758	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	2	30	theme	pectin	526:531	arg1	quantities					461:470	quantities	461:470	quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution)	461:552	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	31	theme	major	1211:1215	arg1	portion					1217:1223	major portion	1211:1223	major portion of the beads	1211:1236	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	32	theme	mucoadhesive	1075:1086	arg1	study					1088:1092	ex vivo mucoadhesive study	1067:1092	ex vivo mucoadhesive study	1067:1092	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	33	theme	mucoadhesive	248:259	arg1	formulation					276:286	mucoadhesive composite bead formulation	248:286	mucoadhesive composite bead formulation (CMB)	248:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	33	theme	mucoadhesive	248:259	arg1	CMB					289:291	CMB	289:291	CMB	289:291	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	5	34	theme	CMB	1311:1313	arg1	testing					1300:1306	accelerated stability testing	1278:1306	accelerated stability testing of CMB(F18)	1278:1318	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	0	35	theme	sustained	32:40	arg1	release					42:48	sustained release	32:48	sustained release	32:48	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	1	36	theme	bead	271:274	arg1	formulation					276:286	mucoadhesive composite bead formulation	248:286	mucoadhesive composite bead formulation (CMB)	248:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	36	theme	bead	271:274	arg1	CMB					289:291	CMB	289:291	CMB	289:291	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	0	37	theme	mucoadhesive	50:61	arg1	beads					73:77	mucoadhesive composite beads	50:77	mucoadhesive composite beads	50:77	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	5	38	dep	revealed	1099:1106	arg1	exhibited					967:975	exhibited	967:975	exhibited significantly higher swelling upon exposure to alkaline media than acidic media	967:1055	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	39	theme	accelerated	1278:1288	arg1	testing					1300:1306	accelerated stability testing	1278:1306	accelerated stability testing of CMB(F18)	1278:1318	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	40	theme	higher	991:996	arg1	swelling					998:1005	significantly higher swelling	977:1005	significantly higher swelling upon exposure to alkaline media	977:1037	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	41	theme	Seed	147:150	arg1	LSM					161:163	LSM	161:163	LSM	161:163	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	41	theme	Seed	147:150	arg1	mucilage					152:159	Linum Seed mucilage	141:159	Linum Seed mucilage(LSM)	141:164	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	5	42	theme	beads	1131:1135	arg1	fraction					1119:1126	major fraction	1113:1126	major fraction of beads	1113:1135	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	43	theme	mucoadhesive	196:207	arg1	blend					132:136	a blend	130:136	a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG)	130:191	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	43	theme	mucoadhesive	196:207	arg1	carriers					209:216	mucoadhesive carriers	196:216	mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB)	196:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	4	44	theme	drug	844:847	arg1	release					849:855	favorable in vitro drug release	825:855	favorable in vitro drug release of CPTB(54.43%) in 12 h	825:879	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	2	45	from	effect	451:456	arg1	release					612:618	%drug release	606:618	%drug release (%DR)	606:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	45	from	effect	451:456	arg1	factors					566:572	response factors	557:572	response factors such as %drug loading (%DL) and %drug release (%DR)	557:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	45	from	effect	451:456	arg1	loading					588:594	%drug loading	582:594	%drug loading (%DL)	582:600	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	46	theme	about	1343:1347	arg1	2.59 years					1349:1358	about 2.59 years	1343:1358	about 2.59 years	1343:1358	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	5	47	theme	CMB	961:963	arg1	's					964:965	CMB's	961:965	CMB's	961:965	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	2	48	from	quantities	461:470	arg1	solution					544:551	curing solution	537:551	curing solution	537:551	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	49	theme	alkaline	1196:1203	arg1	media					1205:1209	7.4pH alkaline media	1190:1209	7.4pH alkaline media	1190:1209	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	2	50	theme	%	606:606	arg1	release					612:618	%drug release	606:618	%drug release (%DR)	606:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	50	theme	%	606:606	arg1	DR					622:623	%DR	621:623	%DR	621:623	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	4	51	dep	in	835:836	arg1	vitro					838:842	vitro	838:842	vitro	838:842	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	1	52	theme	colon	372:376	arg1	region					378:383	the colon region	368:383	the colon region	368:383	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	53	theme	enteric	510:516	arg1	pectin					526:531	enteric polymer pectin	510:531	enteric polymer pectin (in curing solution)	510:552	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	4	54	theme	release	893:899	arg1	kinetics					901:908	the release kinetics	889:908	the release kinetics	889:908	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	4	55	theme	%	802:802	arg1	loading					808:814	maximum %drug loading	794:814	maximum %drug loading(28.94%)	794:822	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	4	55	theme	%	802:802	arg1	%					821:821	28.94%	816:821	28.94%	816:821	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	2	56	theme	%	621:621	arg1	release					612:618	%drug release	606:618	%drug release (%DR)	606:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	56	theme	%	621:621	arg1	DR					622:623	%DR	621:623	%DR	621:623	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	1	57	theme	Leaf	179:182	arg1	gum					184:186	Hibiscus Leaf gum	170:186	Hibiscus Leaf gum(HLG)	170:191	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	57	theme	Leaf	179:182	arg1	HLG					188:190	HLG	188:190	HLG	188:190	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	58	theme	%	582:582	arg1	loading					588:594	%drug loading	582:594	%drug loading (%DL)	582:600	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	58	theme	%	582:582	arg1	DL					598:599	%DL	597:599	%DL	597:599	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	59	theme	mucoadhesive	475:486	arg1	carriers					488:495	mucoadhesive carriers	475:495	mucoadhesive carriers(LSM,HLG)	475:504	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	6	60	theme	ideal	1421:1425	arg1	carriers					1440:1447	ideal mucoadhesive carriers	1421:1447	ideal mucoadhesive carriers	1421:1447	Hence the study confirms that the combination of LSM&HLG as ideal mucoadhesive carriers and can favorably target highly soluble drugs to the colon region.
31374273	2	61	from	pectin	526:531	arg1	solution					544:551	curing solution	537:551	curing solution	537:551	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	62	theme	acidic	1044:1049	arg1	media					1051:1055	acidic media	1044:1055	acidic media	1044:1055	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	63	theme	Class	351:355	arg1	CPTB					341:344	CPTB	341:344	CPTB (BCS Class I drug)	341:363	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	63	theme	Class	351:355	arg1	drug					359:362	BCS Class I drug	347:362	BCS Class I drug	347:362	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	64	theme	quantities	461:470	arg1	effect					451:456	the effect	447:456	the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR)	447:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	6	65	theme	LSM&HLG	1410:1416	arg1	combination					1395:1405	the combination	1391:1405	the combination of LSM&HLG as ideal mucoadhesive carriers	1391:1447	Hence the study confirms that the combination of LSM&HLG as ideal mucoadhesive carriers and can favorably target highly soluble drugs to the colon region.
31374273	2	66	theme	%	597:597	arg1	loading					588:594	%drug loading	582:594	%drug loading (%DL)	582:600	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	66	theme	%	597:597	arg1	DL					598:599	%DL	597:599	%DL	597:599	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	1	67	theme	Linum	141:145	arg1	LSM					161:163	LSM	161:163	LSM	161:163	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	67	theme	Linum	141:145	arg1	mucilage					152:159	Linum Seed mucilage	141:159	Linum Seed mucilage(LSM)	141:164	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	3	68	theme	uniform	673:679	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	68	theme	uniform	673:679	arg1	efficiency					749:758	maximum drug encapsulation efficiency	722:758	maximum drug encapsulation efficiency	722:758	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	68	theme	uniform	673:679	arg1	morphology					710:719	spherical surface morphology	692:719	spherical surface morphology	692:719	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	6	69	theme	colon	1502:1506	arg1	region					1508:1513	the colon region	1498:1513	the colon region	1498:1513	Hence the study confirms that the combination of LSM&HLG as ideal mucoadhesive carriers and can favorably target highly soluble drugs to the colon region.
31374273	4	70	theme	order	922:926	arg1	mechanism					950:958	zero order non-Fickian diffusion mechanism	917:958	zero order non-Fickian diffusion mechanism	917:958	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	2	71	from	solution	544:551	arg1	quantities					461:470	quantities	461:470	quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution)	461:552	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	1	72	theme	mucilage	152:159	arg1	blend					132:136	a blend	130:136	a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG)	130:191	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	72	theme	mucilage	152:159	arg1	carriers					209:216	mucoadhesive carriers	196:216	mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB)	196:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	73	theme	Box-Behnken	405:415	arg1	BBD					424:426	BBD	424:426	BBD	424:426	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	73	theme	Box-Behnken	405:415	arg1	Design					417:422	Box-Behnken Design	405:422	Box-Behnken Design(BBD)	405:427	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	74	theme	curing	537:542	arg1	solution					544:551	curing solution	537:551	curing solution	537:551	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	3	75	theme	spherical	692:700	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	75	theme	spherical	692:700	arg1	morphology					710:719	spherical surface morphology	692:719	spherical surface morphology	692:719	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	6	76	theme	soluble	1481:1487	arg1	drugs					1489:1493	highly soluble drugs	1474:1493	highly soluble drugs	1474:1493	Hence the study confirms that the combination of LSM&HLG as ideal mucoadhesive carriers and can favorably target highly soluble drugs to the colon region.
31374273	1	77	from	release	330:336	arg1	region					378:383	the colon region	368:383	the colon region	368:383	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	3	78	theme	encapsulation	735:747	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	78	theme	encapsulation	735:747	arg1	efficiency					749:758	maximum drug encapsulation efficiency	722:758	maximum drug encapsulation efficiency	722:758	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	2	79	theme	response	557:564	arg1	release					612:618	%drug release	606:618	%drug release (%DR)	606:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	79	theme	response	557:564	arg1	factors					566:572	response factors	557:572	response factors such as %drug loading (%DL) and %drug release (%DR)	557:624	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	2	79	theme	response	557:564	arg1	loading					588:594	%drug loading	582:594	%drug loading (%DL)	582:600	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	5	80	theme	major	1113:1117	arg1	fraction					1119:1126	major fraction	1113:1126	major fraction of beads	1113:1135	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	81	theme	sustained	320:328	arg1	release					330:336	sustained release	320:336	sustained release of CPTB (BCS Class I drug) in the colon region	320:383	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	3	82	theme	maximum	722:728	arg1	size					686:689	uniform bead size	673:689	uniform bead size	673:689	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	3	82	theme	maximum	722:728	arg1	efficiency					749:758	maximum drug encapsulation efficiency	722:758	maximum drug encapsulation efficiency	722:758	CMB prepared by ion-gelation technique showed uniform bead size, spherical surface morphology, maximum drug encapsulation efficiency.
31374273	5	83	theme	beads	1232:1236	arg1	portion					1217:1223	major portion	1211:1223	major portion of the beads	1211:1236	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	1	84	theme	present	104:110	arg1	work					112:115	The present work	100:115	The present work	100:115	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	4	85	theme	favorable	825:833	arg1	release					849:855	favorable in vitro drug release	825:855	favorable in vitro drug release of CPTB(54.43%) in 12 h	825:879	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	0	86	theme	release	42:48	arg1	optimization					16:27	optimization	16:27	optimization	16:27	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	0	86	theme	release	42:48	arg1	Development					0:10	Development	0:10	Development	0:10	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	1	87	theme	composite	261:269	arg1	formulation					276:286	mucoadhesive composite bead formulation	248:286	mucoadhesive composite bead formulation (CMB)	248:292	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	1	87	theme	composite	261:269	arg1	CMB					289:291	CMB	289:291	CMB	289:291	The present work investigates a blend of Linum Seed mucilage(LSM) and Hibiscus Leaf gum(HLG) as mucoadhesive carriers for Capecitabine(CPTB) loaded mucoadhesive composite bead formulation (CMB), in an attempt to achieve sustained release of CPTB (BCS Class I drug) in the colon region.
31374273	2	88	used	used	433:436	arg2	Optimization					386:397	Optimization	386:397	Optimization using Box-Behnken Design(BBD)	386:427	Optimization using Box-Behnken Design(BBD) was used to study the effect of quantities of mucoadhesive carriers(LSM,HLG) and enteric polymer pectin (in curing solution) on response factors such as %drug loading (%DL) and %drug release (%DR).
31374273	0	89	theme	composite	63:71	arg1	beads					73:77	mucoadhesive composite beads	50:77	mucoadhesive composite beads	50:77	Development and optimization of sustained release mucoadhesive composite beads for colon targeting.
31374273	4	90	theme	CPTB	860:863	arg1	release					849:855	favorable in vitro drug release	825:855	favorable in vitro drug release of CPTB(54.43%) in 12 h	825:879	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
31374273	5	91	theme	stability	1290:1298	arg1	testing					1300:1306	accelerated stability testing	1278:1306	accelerated stability testing of CMB(F18)	1278:1318	CMB's exhibited significantly higher swelling upon exposure to alkaline media than acidic media similarly ex vivo mucoadhesive study also revealed that major fraction of beads were washed off within 2 h in 1.2pH media whereas in 7.4pH alkaline media major portion of the beads remain adhered even after 24 h Moreover accelerated stability testing of CMB(F18) revealed shelf life of about 2.59 years.
31374273	4	92	theme	diffusion	940:948	arg1	mechanism					950:958	zero order non-Fickian diffusion mechanism	917:958	zero order non-Fickian diffusion mechanism	917:958	The optimized CMB(F18) exhibited maximum %drug loading(28.94%), favorable in vitro drug release of CPTB(54.43%) in 12 h, where, the release kinetics follow zero order non-Fickian diffusion mechanism.
29401627	3	0	theme	O-glycosylation	524:538	arg1	profiles					540:547	different O-glycosylation profiles	514:547	different O-glycosylation profiles	514:547	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	9	1	theme	basis	1783:1787	arg1	function					1590:1597	function	1590:1597	function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1590:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	1	theme	basis	1783:1787	arg1	structure					1576:1584	the structure	1572:1584	the structure	1572:1584	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	1	2	theme	protein-carbohydrate	166:185	arg1	interactions					187:198	protein-carbohydrate interactions	166:198	protein-carbohydrate interactions	166:198	Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions.
29401627	6	3	with	interaction	1144:1154	arg1	mDectin-2					1161:1169	mDectin-2	1161:1169	mDectin-2	1161:1169	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	6	4	theme	high-mannose	1187:1198	arg1	N-glycans					1200:1208	high-mannose N-glycans	1187:1208	high-mannose N-glycans	1187:1208	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	3	5	theme	mass	567:570	arg1	MS					586:587	MS	586:587	MS	586:587	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	3	5	theme	mass	567:570	arg1	spectrometry					572:583	mass spectrometry	567:583	mass spectrometry (MS)	567:588	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	4	6	theme	terminal	672:679	arg1	glycans					681:687	terminal glycans	672:687	terminal glycans	672:687	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	4	7	theme	glycosylation	613:625	arg1	Modification					591:602	Modification	591:602	Modification	591:602	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	5	8	theme	cell-reporter	973:985	arg1	assay					987:991	a cell-reporter assay	971:991	a cell-reporter assay	971:991	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	8	9	theme	N-glycan	1363:1370	arg1	structures					1372:1381	N-glycan structures	1363:1381	N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3	1363:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	7	10	theme	C-type	1259:1264	arg1	lectins					1266:1272	the 3 C-type lectins	1253:1272	the 3 C-type lectins	1253:1272	Furthermore, we observed Gal-3 binding to the 3 C-type lectins by force spectroscopy.
29401627	9	11	theme	mucin	1804:1808	arg1	recognition					1810:1820	intestinal mucin recognition	1793:1820	intestinal mucin recognition by galectin-3 and C-type lectins	1793:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	4	12	theme	mucin	607:611	arg1	glycosylation					613:625	mucin glycosylation	607:625	mucin glycosylation	607:625	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	6	13	theme	colonic	1096:1102	arg1	Muc					1111:1113	mouse colonic mucin (Muc)-2	1090:1116	mouse colonic mucin (Muc)-2	1090:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	2	14	theme	galectin	413:420	arg1	Gal					423:425	galectin (Gal)-3	413:428	galectin (Gal)-3	413:428	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	2	15	used	used	204:207	arg2	We					201:202	We	201:202	We	201:202	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	3	16	with	mucins	502:507	arg1	profiles					540:547	different O-glycosylation profiles	514:547	different O-glycosylation profiles	514:547	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	0	17	theme	Molecular	0:8	arg1	basis					10:14	Molecular basis	0:14	Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	0:80	Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	1	18	theme	Intestinal	83:92	arg1	mucins					94:99	Intestinal mucins	83:99	Intestinal mucins	83:99	Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions.
29401627	6	19	theme	Muc	1111:1113	arg1	profile					1079:1085	the N-glycosylation profile	1059:1085	the N-glycosylation profile of mouse colonic mucin (Muc)-2	1059:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	2	20	theme	lectin	441:446	arg1	receptors					448:456	C-type lectin receptors	434:456	C-type lectin receptors	434:456	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	4	21	theme	poly-	727:731	arg1	N-acetyllactosamine					733:751	poly- N-acetyllactosamine	727:751	poly- N-acetyllactosamine	727:751	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	5	22	theme	molecule-grabbing	895:911	arg1	nonintegrin-related-1					913:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1	863:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1)	863:943	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	22	theme	molecule-grabbing	895:911	arg1	SIGN-R1					936:942	SIGN-R1	936:942	SIGN-R1	936:942	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	2	23	theme	interaction	325:335	arg1	specificity					306:316	the specificity	302:316	the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors	302:456	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	4	24	theme	glycans	681:687	arg1	loss					664:667	a loss	662:667	a loss of terminal glycans	662:687	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	0	25	theme	intestinal	20:29	arg1	recognition					37:47	intestinal mucin recognition	20:47	intestinal mucin recognition by galectin-3 and C-type lectins	20:80	Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	26	theme	C-type	1840:1845	arg1	lectins					1847:1853	C-type lectins	1840:1853	C-type lectins	1840:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	6	27	theme	mucin	1104:1108	arg1	Muc					1111:1113	mouse colonic mucin (Muc)-2	1090:1116	mouse colonic mucin (Muc)-2	1090:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	9	28	dep	N.	1770:1771	arg1	D.					1743:1744	D.	1743:1744	D.	1743:1744	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	28	dep	N.	1770:1771	arg1	MacKenzie					1732:1740	major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N.	1602:1771	MacKenzie	1732:1740	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	28	dep	N.	1770:1771	arg1	C.					1637:1638	C.	1637:1638	C.	1637:1638	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	29	theme	mucins	1513:1518	arg1	role					1505:1508	the role	1501:1508	the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1501:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	1	30	theme	immune	109:114	arg1	responses					116:124	immune responses	109:124	immune responses	109:124	Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions.
29401627	5	31	theme	specific	863:870	arg1	nonintegrin-related-1					913:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1	863:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1)	863:943	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	31	theme	specific	863:870	arg1	SIGN-R1					936:942	SIGN-R1	936:942	SIGN-R1	936:942	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	32	theme	mouse	809:813	arg1	mDectin					849:855	mouse dendritic cell-associated lectin (mDectin)-2	809:858	mouse dendritic cell-associated lectin (mDectin)-2	809:858	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	4	33	theme	Gal-3	718:722	arg1	interaction					703:713	the interaction	699:713	the interaction of Gal-3 to poly- N-acetyllactosamine	699:751	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
29401627	6	34	theme	mouse	1090:1094	arg1	Muc					1111:1113	mouse colonic mucin (Muc)-2	1090:1116	mouse colonic mucin (Muc)-2	1090:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	2	35	theme	biophysical	251:261	arg1	approaches					279:288	structural, biochemical, biophysical, and cell-based approaches	226:288	structural, biochemical, biophysical, and cell-based approaches	226:288	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	5	36	theme	intercellular	872:884	arg1	nonintegrin-related-1					913:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1	863:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1)	863:943	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	36	theme	intercellular	872:884	arg1	SIGN-R1					936:942	SIGN-R1	936:942	SIGN-R1	936:942	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	0	37	theme	mucin	31:35	arg1	recognition					37:47	intestinal mucin recognition	20:47	intestinal mucin recognition by galectin-3 and C-type lectins	20:80	Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	5	38	theme	dendritic	815:823	arg1	mDectin					849:855	mouse dendritic cell-associated lectin (mDectin)-2	809:858	mouse dendritic cell-associated lectin (mDectin)-2	809:858	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	39	theme	Specific	754:761	arg1	interactions					763:774	Specific interactions	754:774	Specific interactions	754:774	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	2	40	theme	C-type	434:439	arg1	receptors					448:456	C-type lectin receptors	434:456	C-type lectin receptors	434:456	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	5	41	theme	adhesion	886:893	arg1	nonintegrin-related-1					913:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1	863:933	specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1)	863:943	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	41	theme	adhesion	886:893	arg1	SIGN-R1					936:942	SIGN-R1	936:942	SIGN-R1	936:942	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	5	42	theme	cell-associated	825:839	arg1	mDectin					849:855	mouse dendritic cell-associated lectin (mDectin)-2	809:858	mouse dendritic cell-associated lectin (mDectin)-2	809:858	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	2	43	theme	biochemical	238:248	arg1	approaches					279:288	structural, biochemical, biophysical, and cell-based approaches	226:288	structural, biochemical, biophysical, and cell-based approaches	226:288	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	2	44	theme	mammalian	363:371	arg1	lectins					373:379	mammalian lectins	363:379	mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors	363:456	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	5	45	theme	lectin	841:846	arg1	mDectin					849:855	mouse dendritic cell-associated lectin (mDectin)-2	809:858	mouse dendritic cell-associated lectin (mDectin)-2	809:858	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	8	46	theme	recognition	1426:1436	arg1	Gal-3					1448:1452	Gal-3	1448:1452	Gal-3	1448:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	8	46	theme	recognition	1426:1436	arg1	domain					1438:1443	the carbohydrate recognition domain	1409:1443	the carbohydrate recognition domain of Gal-3	1409:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	2	47	theme	structural	226:235	arg1	approaches					279:288	structural, biochemical, biophysical, and cell-based approaches	226:288	structural, biochemical, biophysical, and cell-based approaches	226:288	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	8	48	theme	carbohydrate	1413:1424	arg1	Gal-3					1448:1452	Gal-3	1448:1452	Gal-3	1448:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	8	48	theme	carbohydrate	1413:1424	arg1	domain					1438:1443	the carbohydrate recognition domain	1409:1443	the carbohydrate recognition domain of Gal-3	1409:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	7	49	theme	Gal-3	1236:1240	arg1	binding					1242:1248	Gal-3 binding	1236:1248	Gal-3 binding to the 3 C-type lectins by force spectroscopy	1236:1294	Furthermore, we observed Gal-3 binding to the 3 C-type lectins by force spectroscopy.
29401627	3	50	theme	different	514:522	arg1	profiles					540:547	different O-glycosylation profiles	514:547	different O-glycosylation profiles	514:547	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	5	51	theme	force	1022:1026	arg1	spectroscopy					1028:1039	atomic force spectroscopy	1015:1039	atomic force spectroscopy	1015:1039	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	2	52	theme	approaches	279:288	arg1	combination					211:221	a combination	209:221	a combination of structural, biochemical, biophysical, and cell-based approaches	209:288	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	9	53	theme	immune	1533:1538	arg1	responses					1540:1548	immune responses	1533:1548	immune responses	1533:1548	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	3	54	theme	intestinal	491:500	arg1	mucins					502:507	intestinal mucins	491:507	intestinal mucins with different O-glycosylation profiles	491:547	Gal-3 differentially recognized intestinal mucins with different O-glycosylation profiles, as determined by mass spectrometry (MS).
29401627	5	55	theme	atomic	1015:1020	arg1	spectroscopy					1028:1039	atomic force spectroscopy	1015:1039	atomic force spectroscopy	1015:1039	Specific interactions were observed between mucins and mouse dendritic cell-associated lectin (mDectin)-2 or specific intercellular adhesion molecule-grabbing nonintegrin-related-1 (SIGN-R1), but not mDectin-1, using a cell-reporter assay, as also confirmed by atomic force spectroscopy.
29401627	9	56	theme	new	1554:1556	arg1	insights					1558:1565	new insights	1554:1565	new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1554:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	2	57	theme	cell-based	268:277	arg1	approaches					279:288	structural, biochemical, biophysical, and cell-based approaches	226:288	structural, biochemical, biophysical, and cell-based approaches	226:288	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	6	58	gly	N-glycosylation	1063:1077	arg1	Muc					1111:1113	mouse colonic mucin (Muc)-2	1090:1116	mouse colonic mucin (Muc)-2	1090:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	2	59	theme	mucin	345:349	arg1	glycans					351:357	mucin glycans	345:357	mucin glycans	345:357	We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors.
29401627	6	60	theme	N-glycosylation	1063:1077	arg1	profile					1079:1085	the N-glycosylation profile	1059:1085	the N-glycosylation profile of mouse colonic mucin (Muc)-2	1059:1116	We characterized the N-glycosylation profile of mouse colonic mucin (Muc)-2 by MS and showed that the interaction with mDectin-2 was mediated by high-mannose N-glycans.
29401627	0	61	theme	C-type	67:72	arg1	lectins					74:80	C-type lectins	67:80	C-type lectins	67:80	Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	62	theme	intestinal	1793:1802	arg1	recognition					1810:1820	intestinal mucin recognition	1793:1820	intestinal mucin recognition by galectin-3 and C-type lectins	1793:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	9	63	theme	structural	1480:1489	arg1	basis					1491:1495	a structural basis	1478:1495	a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1478:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	1	64	theme	dendritic	146:154	arg1	cells					156:160	dendritic cells	146:160	dendritic cells	146:160	Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions.
29401627	9	65	theme	N.	1770:1771	arg1	basis					1783:1787	major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis	1602:1787	major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1602:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	8	66	theme	Gal-3	1448:1452	arg1	Gal-3					1448:1452	Gal-3	1448:1452	Gal-3	1448:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	8	66	theme	Gal-3	1448:1452	arg1	domain					1438:1443	the carbohydrate recognition domain	1409:1443	the carbohydrate recognition domain of Gal-3	1409:1452	We showed that mDectin-1, mDectin-2, and SIGN-R1 are decorated by N-glycan structures that can be recognized by the carbohydrate recognition domain of Gal-3.
29401627	7	67	theme	force	1277:1281	arg1	spectroscopy					1283:1294	force spectroscopy	1277:1294	force spectroscopy	1277:1294	Furthermore, we observed Gal-3 binding to the 3 C-type lectins by force spectroscopy.
29401627	9	68	theme	Molecular	1773:1781	arg1	basis					1783:1787	major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis	1602:1787	major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins	1602:1853	These findings provide a structural basis for the role of mucins in mediating immune responses and new insights into the structure and function of major mammalian lectins.-Leclaire, C., Lecointe, K., Gunning, P. A., Tribolo, S., Kavanaugh, D. W., Wittmann, A., Latousakis, D., MacKenzie, D. A., Kawasaki, N., Juge, N. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.
29401627	4	69	theme	chemical	632:639	arg1	treatment					641:649	chemical treatment	632:649	chemical treatment leading to a loss of terminal glycans	632:687	Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine.
30412313	0	0	theme	mechanical	88:97	arg1	properties					99:108	mechanical properties	88:108	mechanical properties	88:108	Effect of methacrylated chitosan incorporated in experimental composite and adhesive on mechanical properties and biofilm formation.
30412313	1	1	theme	restoration	163:173	arg1	lifespan					137:144	The lifespan	133:144	The lifespan of a resin-based restoration	133:173	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	1	1	theme	restoration	163:173	arg1	limited					178:184	limited	178:184	limited	178:184	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	4	2	theme	mechanical	707:716	arg1	properties					718:727	the mechanical properties	703:727	the mechanical properties of the modified materials	703:753	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	6	3	theme	biofilm	1060:1066	arg1	formation					1037:1045	the formation	1033:1045	the formation of S. mutans biofilm	1033:1066	Methacrylated chitosan was incorporated into an experimental composite and adhesive, and the modified materials reduced the formation of S. mutans biofilm.
30412313	3	4	theme	antimicrobial	373:385	arg1	properties					387:396	antimicrobial properties	373:396	antimicrobial properties	373:396	Dental materials with antimicrobial properties may reduce formation of biofilm and thus increase the longevity of restorations.
30412313	4	5	theme	chitosan	539:546	arg1	effect					515:520	the effect	511:520	the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans	511:687	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	6	6	theme	S. mutans	1050:1058	arg1	biofilm					1060:1066	S. mutans biofilm	1050:1066	S. mutans biofilm	1050:1066	Methacrylated chitosan was incorporated into an experimental composite and adhesive, and the modified materials reduced the formation of S. mutans biofilm.
30412313	5	7	from	effect	869:874	arg1	solution					903:910	solution	903:910	solution	903:910	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	10	8	theme	flexural	1400:1407	arg1	values					1418:1423	flexural strength values	1400:1423	flexural strength values	1400:1423	However, flexural strength values still met the requirement in the ISO standard.
30412313	4	9	theme	modified	736:743	arg1	materials					745:753	the modified materials	732:753	the modified materials	732:753	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	4	10	from	effect	515:520	arg1	growth					658:663	biofilm growth	650:663	biofilm growth of Streptococcus mutans	650:687	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	4	11	theme	mutans	682:687	arg1	growth					658:663	biofilm growth	650:663	biofilm growth of Streptococcus mutans	650:687	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	0	12	theme	biofilm	114:120	arg1	formation					122:130	biofilm formation	114:130	biofilm formation	114:130	Effect of methacrylated chitosan incorporated in experimental composite and adhesive on mechanical properties and biofilm formation.
30412313	9	13	theme	CH-MA	1384:1388	arg1	CH-MA					1384:1388	CH-MA	1384:1388	CH-MA	1384:1388	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	9	13	theme	CH-MA	1384:1388	arg1	amounts					1373:1379	increasing amounts	1362:1379	increasing amounts of CH-MA	1362:1388	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	2	14	theme	tooth-material	269:282	arg1	interface					284:292	the tooth-material interface	265:292	the tooth-material interface	265:292	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	3	15	theme	restorations	465:476	arg1	longevity					452:460	the longevity	448:460	the longevity of restorations	448:476	Dental materials with antimicrobial properties may reduce formation of biofilm and thus increase the longevity of restorations.
30412313	7	16	theme	adhesives	1135:1143	arg1	strength					1119:1126	the bond strength	1110:1126	the bond strength of the adhesives	1110:1143	The incorporation of CH-MA did not alter the bond strength of the adhesives.
30412313	5	17	theme	antibacterial	855:867	arg1	effect					869:874	the antibacterial effect	851:874	the antibacterial effect of the modified polymer in solution	851:910	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	0	18	theme	chitosan	24:31	arg1	Effect					0:5	Effect	0:5	Effect of methacrylated chitosan	0:31	Effect of methacrylated chitosan incorporated in experimental composite and adhesive on mechanical properties and biofilm formation.
30412313	10	19	theme	standard	1462:1469	arg1	ISO					1458:1460	the ISO standard	1454:1469	the ISO standard	1454:1469	However, flexural strength values still met the requirement in the ISO standard.
30412313	2	20	theme	etiological	309:319	arg1	formation					252:260	Biofilm formation	244:260	Biofilm formation at the tooth-material interface	244:292	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	2	20	theme	etiological	309:319	arg1	agent					321:325	a necessary etiological agent	297:325	a necessary etiological agent for caries development	297:348	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	4	21	theme	biofilm	650:656	arg1	growth					658:663	biofilm growth	650:663	biofilm growth of Streptococcus mutans	650:687	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	0	22	theme	methacrylated	10:22	arg1	chitosan					24:31	methacrylated chitosan	10:31	methacrylated chitosan	10:31	Effect of methacrylated chitosan incorporated in experimental composite and adhesive on mechanical properties and biofilm formation.
30412313	2	23	theme	necessary	299:307	arg1	formation					252:260	Biofilm formation	244:260	Biofilm formation at the tooth-material interface	244:292	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	2	23	theme	necessary	299:307	arg1	agent					321:325	a necessary etiological agent	297:325	a necessary etiological agent for caries development	297:348	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	5	24	theme	modified	883:890	arg1	polymer					892:898	the modified polymer	879:898	the modified polymer	879:898	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	4	25	theme	polymeric	579:587	arg1	network					589:595	the polymeric network	575:595	the polymeric network of an experimental dental composite and adhesive	575:644	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	7	26	theme	bond	1114:1117	arg1	strength					1119:1126	the bond strength	1110:1126	the bond strength of the adhesives	1110:1143	The incorporation of CH-MA did not alter the bond strength of the adhesives.
30412313	6	27	theme	Methacrylated	913:925	arg1	chitosan					927:934	Methacrylated chitosan	913:934	Methacrylated chitosan	913:934	Methacrylated chitosan was incorporated into an experimental composite and adhesive, and the modified materials reduced the formation of S. mutans biofilm.
30412313	3	28	with	materials	358:366	arg1	properties					387:396	antimicrobial properties	373:396	antimicrobial properties	373:396	Dental materials with antimicrobial properties may reduce formation of biofilm and thus increase the longevity of restorations.
30412313	5	29	theme	polymer	892:898	arg1	effect					869:874	the antibacterial effect	851:874	the antibacterial effect of the modified polymer in solution	851:910	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	5	30	theme	biofilm	825:831	arg1	studies					833:839	biofilm studies	825:839	biofilm studies	825:839	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	6	31	theme	modified	1006:1013	arg1	materials					1015:1023	the modified materials	1002:1023	the modified materials	1002:1023	Methacrylated chitosan was incorporated into an experimental composite and adhesive, and the modified materials reduced the formation of S. mutans biofilm.
30412313	8	32	theme	mechanical	1256:1265	arg1	properties					1267:1276	the mechanical properties	1252:1276	the mechanical properties of the material	1252:1292	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	10	33	theme	strength	1409:1416	arg1	values					1418:1423	flexural strength values	1400:1423	flexural strength values	1400:1423	However, flexural strength values still met the requirement in the ISO standard.
30412313	3	34	theme	biofilm	422:428	arg1	formation					409:417	formation	409:417	formation of biofilm	409:428	Dental materials with antimicrobial properties may reduce formation of biofilm and thus increase the longevity of restorations.
30412313	5	35	theme	chitosan	799:806	arg1	methacrylation					760:773	The methacrylation	756:773	The methacrylation of low-molecular-weight chitosan	756:806	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	7	36	theme	CH-MA	1090:1094	arg1	incorporation					1073:1085	The incorporation	1069:1085	The incorporation of CH-MA	1069:1094	The incorporation of CH-MA did not alter the bond strength of the adhesives.
30412313	4	37	theme	composite	623:631	arg1	network					589:595	the polymeric network	575:595	the polymeric network of an experimental dental composite and adhesive	575:644	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	9	38	theme	composite	1337:1345	arg1	flexural strength					1312:1328	flexural strength	1312:1328	flexural strength	1312:1328	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	9	38	theme	composite	1337:1345	arg1	hardness					1299:1306	hardness	1299:1306	hardness	1299:1306	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	10	39	from	requirement	1439:1449	arg1	ISO					1458:1460	the ISO standard	1454:1469	the ISO standard	1454:1469	However, flexural strength values still met the requirement in the ISO standard.
30412313	2	40	from	interface	284:292	arg1	formation					252:260	Biofilm formation	244:260	Biofilm formation at the tooth-material interface	244:292	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	2	40	from	interface	284:292	arg1	agent					321:325	a necessary etiological agent	297:325	a necessary etiological agent for caries development	297:348	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	8	41	theme	CH-MA	1169:1173	arg1	amount					1159:1164	the amount	1155:1164	the amount of CH-MA in composite that is required to elicit an antibacterial response	1155:1239	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	8	41	theme	CH-MA	1169:1173	arg1	CH-MA					1169:1173	CH-MA	1169:1173	CH-MA	1169:1173	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	4	42	theme	materials	745:753	arg1	properties					718:727	the mechanical properties	703:727	the mechanical properties of the modified materials	703:753	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	8	43	from	amount	1159:1164	arg1	composite					1178:1186	composite	1178:1186	composite that is required to elicit an antibacterial response	1178:1239	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	9	44	theme	increasing	1362:1371	arg1	CH-MA					1384:1388	CH-MA	1384:1388	CH-MA	1384:1388	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	9	44	theme	increasing	1362:1371	arg1	amounts					1373:1379	increasing amounts	1362:1379	increasing amounts of CH-MA	1362:1388	The hardness and flexural strength of the composite decreased with increasing amounts of CH-MA.
30412313	8	45	theme	antibacterial	1218:1230	arg1	response					1232:1239	an antibacterial response	1215:1239	an antibacterial response	1215:1239	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	2	46	theme	caries	331:336	arg1	development					338:348	caries development	331:348	caries development	331:348	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	1	47	theme	secondary	226:234	arg1	caries					236:241	secondary caries	226:241	secondary caries	226:241	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	1	47	theme	secondary	226:234	arg1	reason					201:206	the main reason	192:206	the main reason for failure	192:218	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	5	48	theme	low-molecular-weight	778:797	arg1	chitosan					799:806	low-molecular-weight chitosan	778:806	low-molecular-weight chitosan	778:806	The methacrylation of low-molecular-weight chitosan was achieved and biofilm studies confirmed the antibacterial effect of the modified polymer in solution.
30412313	1	49	theme	main	196:199	arg1	caries					236:241	secondary caries	226:241	secondary caries	226:241	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	1	49	theme	main	196:199	arg1	reason					201:206	the main reason	192:206	the main reason for failure	192:218	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	8	50	theme	material	1285:1292	arg1	properties					1267:1276	the mechanical properties	1252:1276	the mechanical properties of the material	1252:1292	However, the amount of CH-MA in composite that is required to elicit an antibacterial response challenges the mechanical properties of the material.
30412313	3	51	theme	Dental	351:356	arg1	materials					358:366	Dental materials	351:366	Dental materials with antimicrobial properties	351:396	Dental materials with antimicrobial properties may reduce formation of biofilm and thus increase the longevity of restorations.
30412313	4	52	theme	methacrylated	525:537	arg1	CH-MA					549:553	CH-MA	549:553	CH-MA	549:553	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	4	52	theme	methacrylated	525:537	arg1	chitosan					539:546	methacrylated chitosan	525:546	methacrylated chitosan (CH-MA)	525:554	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
30412313	2	53	theme	Biofilm	244:250	arg1	formation					252:260	Biofilm formation	244:260	Biofilm formation at the tooth-material interface	244:292	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	2	53	theme	Biofilm	244:250	arg1	agent					321:325	a necessary etiological agent	297:325	a necessary etiological agent for caries development	297:348	Biofilm formation at the tooth-material interface is a necessary etiological agent for caries development.
30412313	1	54	theme	resin-based	151:161	arg1	restoration					163:173	a resin-based restoration	149:173	a resin-based restoration	149:173	The lifespan of a resin-based restoration is limited, with the main reason for failure being secondary caries.
30412313	4	55	theme	adhesive	637:644	arg1	network					589:595	the polymeric network	575:595	the polymeric network of an experimental dental composite and adhesive	575:644	This study aimed to investigate the effect of methacrylated chitosan (CH-MA), incorporated into the polymeric network of an experimental dental composite and adhesive, on biofilm growth of Streptococcus mutans and to assess the mechanical properties of the modified materials.
31362412	4	0	theme	high	570:573	arg1	weight					585:590	high molecular weight fucoidan (HMWF)	570:606	high molecular weight fucoidan (HMWF)	570:606	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	6	1	theme	cell	930:933	arg1	cytotoxicity					935:946	NK cell cytotoxicity	927:946	NK cell cytotoxicity	927:946	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	5	2	theme	high	800:803	arg1	function					805:812	a relatively high function	787:812	a relatively high function	787:812	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	7	3	theme	tumor	1100:1104	arg1	TNF					1123:1125	TNF	1123:1125	TNF	1123:1125	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	7	3	theme	tumor	1100:1104	arg1	factor					1115:1120	tumor necrosis factor (TNF)-α	1100:1128	tumor necrosis factor (TNF)-α	1100:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	7	4	theme	IL-12	1069:1073	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	0	5	from	Characterization	0:15	arg1	Sporophyll					98:107	the Sporophyll	94:107	the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	94:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	2	6	theme	diseases	355:362	arg1	pathogenesis					331:342	pathogenesis	331:342	pathogenesis	331:342	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	2	6	theme	diseases	355:362	arg1	pathophysiology					311:325	pathophysiology	311:325	pathophysiology	311:325	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	4	7	theme	chemical	546:553	arg1	composition					555:565	the chemical composition	542:565	the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan	542:640	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	2	8	theme	various	347:353	arg1	diseases					355:362	various diseases	347:362	various diseases affecting the immune system	347:390	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	0	9	theme	pinnatifida	120:130	arg1	Sporophyll					98:107	the Sporophyll	94:107	the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	94:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	8	10	theme	effective	1201:1209	arg1	immunostimulant					1211:1225	an effective immunostimulant	1198:1225	an effective immunostimulant under immunosuppressive conditions	1198:1260	This study suggests that HMWF acts as an effective immunostimulant under immunosuppressive conditions.
31362412	8	10	theme	effective	1201:1209	arg1	HMWF					1185:1188	HMWF	1185:1188	HMWF	1185:1188	This study suggests that HMWF acts as an effective immunostimulant under immunosuppressive conditions.
31362412	0	11	theme	Undaria	112:118	arg1	pinnatifida					120:130	Undaria pinnatifida	112:130	Undaria pinnatifida	112:130	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	3	12	used	used	417:420	arg2	immunostimulators					425:441	immunostimulators	425:441	immunostimulators	425:441	Immunomodulators can be used as immunostimulators to reduce the side effects of drugs that induce immunosuppression.
31362412	3	12	used	used	417:420	arg2	Immunomodulators					393:408	Immunomodulators	393:408	Immunomodulators	393:408	Immunomodulators can be used as immunostimulators to reduce the side effects of drugs that induce immunosuppression.
31362412	3	13	theme	side	457:460	arg1	effects					462:468	the side effects	453:468	the side effects of drugs that induce immunosuppression	453:507	Immunomodulators can be used as immunostimulators to reduce the side effects of drugs that induce immunosuppression.
31362412	5	14	contain	has	783:785	arg1	HMWF					771:774	HMWF	771:774	HMWF	771:774	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	5	14	contain	has	783:785	arg2	function					805:812	a relatively high function	787:812	a relatively high function	787:812	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	7	15	theme	interferon	1076:1085	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	5	16	theme	animal	867:872	arg1	models					874:879	immunosuppressed animal models	850:879	immunosuppressed animal models	850:879	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	4	17	theme	molecular	616:624	arg1	weight					626:631	low molecular weight	612:631	low molecular weight	612:631	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	2	18	theme	immune	378:383	arg1	system					385:390	the immune system	374:390	the immune system	374:390	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	0	19	theme	Molecular	54:62	arg1	Fraction					80:87	High Molecular Weight Fucoidan Fraction	49:87	High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	49:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	2	20	dep	pathophysiology	311:325	arg1	the					307:309	the	307:309	the	307:309	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	4	21	theme	low	612:614	arg1	weight					626:631	low molecular weight	612:631	low molecular weight	612:631	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	0	22	theme	Cyclophosphamide-Induced	135:158	arg1	Mice					177:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	23	theme	Immunomodulatory	21:36	arg1	Effects					38:44	Immunomodulatory Effects	21:44	Immunomodulatory Effects	21:44	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	5	24	theme	HMWF	771:774	arg1	immunostimulator					830:845	an immunostimulator	827:845	an immunostimulator in immunosuppressed animal models	827:879	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	5	24	theme	HMWF	771:774	arg1	effectiveness					754:766	the effectiveness	750:766	the effectiveness of HMWF, which has a relatively high function in vitro,	750:822	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	6	25	theme	NK	927:928	arg1	cytotoxicity					935:946	NK cell cytotoxicity	927:946	NK cell cytotoxicity	927:946	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	5	26	from	immunostimulator	830:845	arg1	models					874:879	immunosuppressed animal models	850:879	immunosuppressed animal models	850:879	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	0	27	theme	High	49:52	arg1	Fraction					80:87	High Molecular Weight Fucoidan Fraction	49:87	High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	49:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	4	28	theme	weight	626:631	arg1	composition					555:565	the chemical composition	542:565	the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan	542:640	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	7	29	theme	IL-5	1063:1066	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	8	30	theme	immunosuppressive	1233:1249	arg1	conditions					1251:1260	immunosuppressive conditions	1233:1260	immunosuppressive conditions	1233:1260	This study suggests that HMWF acts as an effective immunostimulant under immunosuppressive conditions.
31362412	7	31	theme	necrosis	1106:1113	arg1	TNF					1123:1125	TNF	1123:1125	TNF	1123:1125	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	7	31	theme	necrosis	1106:1113	arg1	factor					1115:1120	tumor necrosis factor (TNF)-α	1100:1128	tumor necrosis factor (TNF)-α	1100:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	6	32	theme	granzyme	952:959	arg1	B					961:961	granzyme B	952:961	granzyme B release	952:969	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	0	33	theme	Immunosuppressed	160:175	arg1	Mice					177:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	4	34	theme	cell-derived	694:705	arg1	functions					661:669	their functions	655:669	their functions	655:669	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	34	theme	cell-derived	694:705	arg1	immunostimulators					707:723	natural killer (NK) cell-derived immunostimulators	674:723	natural killer (NK) cell-derived immunostimulators	674:723	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	0	35	theme	Weight	64:69	arg1	Fraction					80:87	High Molecular Weight Fucoidan Fraction	49:87	High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	49:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	4	36	dep	weight	585:590	arg1	fucoidan					592:599	fucoidan	592:599	high molecular weight fucoidan (HMWF)	570:606	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	36	dep	weight	585:590	arg1	HMWF					602:605	HMWF	602:605	HMWF	602:605	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	36	dep	weight	585:590	arg1	fucoidan					633:640	fucoidan	633:640	fucoidan	633:640	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	7	37	theme	IL-4	1057:1060	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	2	38	theme	complex	274:280	arg1	mechanism					282:290	a complex mechanism	272:290	a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system	272:390	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	2	38	theme	complex	274:280	arg1	It					266:267	It	266:267	It	266:267	It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system.
31362412	3	39	theme	drugs	473:477	arg1	effects					462:468	the side effects	453:468	the side effects of drugs that induce immunosuppression	453:507	Immunomodulators can be used as immunostimulators to reduce the side effects of drugs that induce immunosuppression.
31362412	6	40	theme	group	986:990	arg1	level					992:996	the control group level	974:996	the control group level	974:996	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	4	41	theme	molecular	575:583	arg1	weight					585:590	high molecular weight fucoidan (HMWF)	570:606	high molecular weight fucoidan (HMWF)	570:606	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	6	42	theme	control	978:984	arg1	level					992:996	the control group level	974:996	the control group level	974:996	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	7	43	theme	IL-2	1051:1054	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	7	44	theme	factor	1115:1120	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	4	45	theme	natural	674:680	arg1	NK					690:691	NK	690:691	NK	690:691	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	45	theme	natural	674:680	arg1	killer					682:687	natural killer	674:687	natural killer (NK) cell-derived immunostimulators	674:723	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	46	theme	weight	585:590	arg1	composition					555:565	the chemical composition	542:565	the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan	542:640	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	47	link	cell-derived	694:705	arg1	functions					661:669	their functions	655:669	their functions	655:669	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	47	link	cell-derived	694:705	arg1	immunostimulators					707:723	natural killer (NK) cell-derived immunostimulators	674:723	natural killer (NK) cell-derived immunostimulators	674:723	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	7	48	theme	interleukin	1030:1040	arg1	expression					1016:1025	the expression	1012:1025	the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1012:1128	In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen.
31362412	0	49	theme	Fraction	80:87	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	49	theme	Fraction	80:87	arg1	Effects					38:44	Immunomodulatory Effects	21:44	Immunomodulatory Effects	21:44	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	50	from	Sporophyll	98:107	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	50	from	Sporophyll	98:107	arg1	Effects					38:44	Immunomodulatory Effects	21:44	Immunomodulatory Effects	21:44	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	50	from	Sporophyll	98:107	arg1	Fraction					80:87	High Molecular Weight Fucoidan Fraction	49:87	High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	49:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	50	from	Sporophyll	98:107	arg1	Mice					177:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Cyclophosphamide-Induced Immunosuppressed Mice	135:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	6	51	theme	B	961:961	arg1	release					963:969	granzyme B release	952:969	granzyme B release	952:969	In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level.
31362412	0	52	theme	Fucoidan	71:78	arg1	Fraction					80:87	High Molecular Weight Fucoidan Fraction	49:87	High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	49:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	0	53	from	Effects	38:44	arg1	Sporophyll					98:107	the Sporophyll	94:107	the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice	94:180	Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice.
31362412	5	54	theme	immunosuppressed	850:865	arg1	models					874:879	immunosuppressed animal models	850:879	immunosuppressed animal models	850:879	We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models.
31362412	4	55	theme	killer	682:687	arg1	functions					661:669	their functions	655:669	their functions	655:669	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31362412	4	55	theme	killer	682:687	arg1	immunostimulators					707:723	natural killer (NK) cell-derived immunostimulators	674:723	natural killer (NK) cell-derived immunostimulators	674:723	In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro.
31574365	2	0	theme	sufficient	463:472	arg1	sites					482:486	sufficient binding sites	463:486	sufficient binding sites for adsorption of Cu(II)	463:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	5	1	theme	HIPS/SA	743:749	arg1	hydrogel					751:758	HIPS/SA hydrogel	743:758	HIPS/SA hydrogel	743:758	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	2	2	with	endowed	447:453	arg1	sites					482:486	sufficient binding sites	463:486	sufficient binding sites for adsorption of Cu(II)	463:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	4	3	theme	adsorption	663:672	arg1	capacity					674:681	adsorption capacity	663:681	adsorption capacity	663:681	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	5	4	theme	hydrogel	751:758	arg1	isotherms					717:725	adsorption isotherms	706:725	adsorption isotherms	706:725	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	5	4	theme	hydrogel	751:758	arg1	kinetics					731:738	kinetics	731:738	kinetics	731:738	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	2	5	dep	structure	436:444	arg1	endowed					447:453	endowed	447:453	endowed	447:453	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	1	6	theme	novel	143:147	arg1	hydrogel					187:194	A novel polysaccharide-based thermoresponsive hydrogel	141:194	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS)	141:273	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	2	7	theme	Cu	506:507	arg1	adsorption					492:501	adsorption	492:501	adsorption of Cu(II)	492:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	4	8	theme	Cu	639:640	arg1	concentration					646:658	initial Cu(II) concentration	631:658	initial Cu(II) concentration	631:658	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	5	9	theme	adsorption	706:715	arg1	isotherms					717:725	adsorption isotherms	706:725	adsorption isotherms	706:725	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	0	10	from	solution	131:138	arg1	removal					100:106	the removal	96:106	the removal of Cu(II) from aqueous solution	96:138	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	5	11	theme	maximum	882:888	arg1	25.81 mg/g					914:923	25.81 mg/g	914:923	25.81 mg/g	914:923	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	5	11	theme	maximum	882:888	arg1	capacity					901:908	the maximum adsorption capacity	878:908	the maximum adsorption capacity	878:908	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	6	12	theme	adsorption	1156:1165	arg1	higher					1197:1202	higher	1197:1202	higher	1197:1202	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	6	12	theme	adsorption	1156:1165	arg1	capacity					1167:1174	the adsorption capacity	1152:1174	the adsorption capacity for Cu(II)	1152:1185	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	1	13	theme	polysaccharide-based	149:168	arg1	hydrogel					187:194	A novel polysaccharide-based thermoresponsive hydrogel	141:194	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS)	141:273	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	0	14	theme	Cu	111:112	arg1	removal					100:106	the removal	96:106	the removal of Cu(II) from aqueous solution	96:138	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	6	15	theme	thermoresponsive	1066:1081	arg1	property					1083:1090	its thermoresponsive property	1062:1090	its thermoresponsive property	1062:1090	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	1	16	theme	thermoresponsive	170:185	arg1	hydrogel					187:194	A novel polysaccharide-based thermoresponsive hydrogel	141:194	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS)	141:273	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	3	17	theme	thermoresponsive	529:544	arg1	behavior					565:572	The reversible thermoresponsive swelling-shrinking behavior	514:572	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel	514:592	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel was discussed.
31574365	5	18	theme	pseudo-second-order	833:851	arg1	models					853:858	pseudo-second-order models	833:858	pseudo-second-order models	833:858	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	5	19	theme	Cu	796:797	arg1	adsorption					782:791	the adsorption	778:791	the adsorption of Cu(II)	778:801	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	3	20	theme	swelling-shrinking	546:563	arg1	behavior					565:572	The reversible thermoresponsive swelling-shrinking behavior	514:572	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel	514:592	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel was discussed.
31574365	2	21	theme	porous	368:373	arg1	structure					375:383	porous structure	368:383	porous structure	368:383	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	0	22	theme	alginate/starch	32:46	arg1	hydrogel					64:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	6	23	theme	regeneration	1130:1141	arg1	feasibility					1115:1125	the feasibility	1111:1125	the feasibility of regeneration	1111:1141	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	2	24	theme	abundant	401:408	arg1	groups					418:423	the abundant carboxy groups	397:423	the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II)	397:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	2	25	theme	carboxy	410:416	arg1	groups					418:423	the abundant carboxy groups	397:423	the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II)	397:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	1	26	contain	containing	196:205	arg1	hydrogel					187:194	A novel polysaccharide-based thermoresponsive hydrogel	141:194	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS)	141:273	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	1	26	contain	containing	196:205	arg2	SA					224:225	SA	224:225	SA	224:225	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	1	26	contain	containing	196:205	arg2	alginate					214:221	sodium alginate	207:221	sodium alginate (SA)	207:226	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	1	26	contain	containing	196:205	arg2	starch					261:266	starch	261:266	starch	261:266	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	1	26	contain	containing	196:205	arg2	HIPS					269:272	HIPS	269:272	HIPS	269:272	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	3	27	theme	reversible	518:527	arg1	behavior					565:572	The reversible thermoresponsive swelling-shrinking behavior	514:572	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel	514:592	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel was discussed.
31574365	0	28	theme	composite	54:62	arg1	hydrogel					64:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	6	29	theme	HIPS/SA	940:946	arg1	hydrogel					948:955	HIPS/SA hydrogel	940:955	HIPS/SA hydrogel	940:955	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	0	30	theme	aqueous	123:129	arg1	solution					131:138	aqueous solution	123:138	aqueous solution	123:138	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	3	31	theme	HIPS/SA	577:583	arg1	hydrogel					585:592	HIPS/SA hydrogel	577:592	HIPS/SA hydrogel	577:592	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel was discussed.
31574365	4	32	from	effects	613:619	arg1	capacity					674:681	adsorption capacity	663:681	adsorption capacity	663:681	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	6	33	theme	acid	1033:1036	arg1	amounts					1002:1008	only small amounts	991:1008	only small amounts of dilute hydrochloric acid	991:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	6	33	theme	acid	1033:1036	arg1	acid					1033:1036	dilute hydrochloric acid	1013:1036	dilute hydrochloric acid	1013:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	0	34	theme	ether	48:52	arg1	hydrogel					64:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	3	35	theme	hydrogel	585:592	arg1	behavior					565:572	The reversible thermoresponsive swelling-shrinking behavior	514:572	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel	514:592	The reversible thermoresponsive swelling-shrinking behavior of HIPS/SA hydrogel was discussed.
31574365	4	36	theme	pH	624:625	arg1	effects					613:619	The effects	609:619	The effects of pH and initial Cu(II) concentration on adsorption capacity	609:681	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	5	37	dep	isotherms	717:725	arg1	The					702:704	The	702:704	The	702:704	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	2	38	theme	HIPS/SA	332:338	arg1	hydrogel					340:347	HIPS/SA hydrogel	332:347	HIPS/SA hydrogel	332:347	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	0	39	theme	hydrogel	64:71	arg1	Preparation					0:10	Preparation	0:10	Preparation of thermoresponsive alginate/starch ether composite hydrogel	0:71	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	0	39	theme	hydrogel	64:71	arg1	application					81:91	its application	77:91	its application to the removal of Cu(II) from aqueous solution	77:138	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	5	40	theme	adsorption	890:899	arg1	25.81 mg/g					914:923	25.81 mg/g	914:923	25.81 mg/g	914:923	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	5	40	theme	adsorption	890:899	arg1	capacity					901:908	the maximum adsorption capacity	878:908	the maximum adsorption capacity	878:908	The adsorption isotherms and kinetics of HIPS/SA hydrogel demonstrated that the adsorption of Cu(II) was subjected to Langmuir and pseudo-second-order models respectively, and the maximum adsorption capacity was 25.81 mg/g.
31574365	6	41	theme	dilute	1013:1018	arg1	acid					1033:1036	dilute hydrochloric acid	1013:1036	dilute hydrochloric acid	1013:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	6	42	theme	short	1047:1051	arg1	time					1053:1056	a short time	1045:1056	a short time for its thermoresponsive property	1045:1090	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	6	43	theme	hydrochloric	1020:1031	arg1	acid					1033:1036	dilute hydrochloric acid	1013:1036	dilute hydrochloric acid	1013:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	1	44	theme	sodium	207:212	arg1	alginate					214:221	sodium alginate	207:221	sodium alginate (SA)	207:226	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	1	44	theme	sodium	207:212	arg1	SA					224:225	SA	224:225	SA	224:225	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	4	45	theme	concentration	646:658	arg1	effects					613:619	The effects	609:619	The effects of pH and initial Cu(II) concentration on adsorption capacity	609:681	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	4	46	theme	initial	631:637	arg1	concentration					646:658	initial Cu(II) concentration	631:658	initial Cu(II) concentration	631:658	The effects of pH and initial Cu(II) concentration on adsorption capacity were investigated.
31574365	2	47	theme	binding	474:480	arg1	sites					482:486	sufficient binding sites	463:486	sufficient binding sites for adsorption of Cu(II)	463:511	HIPS/SA hydrogel showed network and porous structure, as well as the abundant carboxy groups inside the structure, endowed it with sufficient binding sites for adsorption of Cu(II).
31574365	1	48	theme	aqueous	314:320	arg1	solution					322:329	aqueous solution	314:329	aqueous solution	314:329	A novel polysaccharide-based thermoresponsive hydrogel containing sodium alginate (SA) and 2-hydroxy-3-isopropoxypropyl starch (HIPS) was developed for removing Cu(II) from aqueous solution.
31574365	0	49	theme	thermoresponsive	15:30	arg1	hydrogel					64:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	thermoresponsive alginate/starch ether composite hydrogel	15:71	Preparation of thermoresponsive alginate/starch ether composite hydrogel and its application to the removal of Cu(II) from aqueous solution.
31574365	6	50	theme	small	996:1000	arg1	amounts					1002:1008	only small amounts	991:1008	only small amounts of dilute hydrochloric acid	991:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31574365	6	50	theme	small	996:1000	arg1	acid					1033:1036	dilute hydrochloric acid	1013:1036	dilute hydrochloric acid	1013:1036	Additionally, HIPS/SA hydrogel could be successfully desorbed by only small amounts of dilute hydrochloric acid within a short time for its thermoresponsive property, it also exhibited the feasibility of regeneration, because the adsorption capacity for Cu(II) was still higher than 15.23 ± 0.27 mg/g even after five cycles.
31887386	10	0	theme	table	1884:1888	arg1	blade					1894:1898	a table fan blade	1882:1898	a table fan blade	1882:1898	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	10	0	theme	table	1884:1888	arg1	guard					1911:1915	an engine guard	1901:1915	an engine guard for two-wheelers and self-motor guard for four wheelers	1901:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	2	1	theme	composite	344:352	arg1	materials					354:362	the composite materials	340:362	the composite materials	340:362	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	2	1	theme	composite	344:352	arg1	reinforcements					447:460	recyclable and biodegradable reinforcements	418:460	recyclable and biodegradable reinforcements	418:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	9	2	theme	filler-based	1622:1633	arg1	composites					1644:1653	the natural filler-based (DSF-VE) composites	1610:1653	the natural filler-based (DSF-VE) composites	1610:1653	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	9	3	theme	DSF-VE	1636:1641	arg1	composites					1644:1653	the natural filler-based (DSF-VE) composites	1610:1653	the natural filler-based (DSF-VE) composites	1610:1653	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	4	4	theme	seed	651:654	arg1	composites					695:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	3	5	theme	solid	546:550	arg1	waste					552:556	bio solid waste	542:556	bio solid waste (date seed filler)	542:575	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	3	5	theme	solid	546:550	arg1	filler					569:574	date seed filler	559:574	date seed filler	559:574	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	1	6	theme	polymer	174:180	arg1	composites					182:191	fibre reinforced polymer composites	157:191	fibre reinforced polymer composites	157:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	6	7	theme	DSF-VE	1157:1162	arg1	composites					1164:1173	the DSF-VE composites	1153:1173	the DSF-VE composites	1153:1173	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	4	8	theme	reinforced	663:672	arg1	DSF-VE					687:692	DSF-VE	687:692	DSF-VE	687:692	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	4	8	theme	reinforced	663:672	arg1	ester					680:684	reinforced vinyl ester	663:684	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	2	9	theme	mechanical	303:312	arg1	properties					326:335	the inherent mechanical and thermal properties	290:335	the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements	290:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	9	10	theme	thermal	1665:1671	arg1	stability					1673:1681	thermal stability	1665:1681	thermal stability	1665:1681	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	4	11	with	technique	763:771	arg1	loadings					794:801	varying fillers loadings	778:801	varying fillers loadings from 5% to 50%	778:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	3	12	theme	seed	564:567	arg1	waste					552:556	bio solid waste	542:556	bio solid waste (date seed filler)	542:575	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	3	12	theme	seed	564:567	arg1	filler					569:574	date seed filler	559:574	date seed filler	559:574	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	4	13	from	%	816:816	arg1	loadings					794:801	varying fillers loadings	778:801	varying fillers loadings from 5% to 50%	778:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	4	14	dep	%	809:809	arg1	to					811:812	to	811:812	to	811:812	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	7	15	from	results	1200:1206	arg1	evident					1183:1189	evident	1183:1189	evident	1183:1189	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	3	16	theme	vinyl	581:585	arg1	ester					587:591	vinyl ester	581:591	vinyl ester	581:591	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	5	17	theme	water	876:880	arg1	absorption					882:891	water absorption	876:891	water absorption	876:891	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	1	18	theme	numerous	125:132	arg1	applications					141:152	numerous better applications	125:152	numerous better applications of fibre reinforced polymer composites	125:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	8	19	theme	deflection	1447:1456	arg1	temperatures					1458:1469	the heat deflection temperatures	1438:1469	the heat deflection temperatures of DSF-VE composites	1438:1490	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	4	20	theme	ester	680:684	arg1	composites					695:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	0	21	theme	thermal	54:60	arg1	properties					62:71	mechanical and thermal properties	39:71	mechanical and thermal properties of date palm seed/vinyl ester composites	39:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	2	22	theme	Many	238:241	arg1	studies					243:249	Many studies	238:249	Many studies	238:249	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	9	23	theme	polymerisation	1720:1733	arg1	temperature					1743:1753	the polymerisation process temperature	1716:1753	the polymerisation process temperature	1716:1753	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	5	24	dep	thermal	897:903	arg1	temperature					922:932	heat deflection temperature	906:932	heat deflection temperature	906:932	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	5	24	dep	thermal	897:903	arg1	analysis					957:964	thermo-gravimetric analysis	938:964	thermo-gravimetric analysis	938:964	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	1	25	theme	fibre	157:161	arg1	composites					182:191	fibre reinforced polymer composites	157:191	fibre reinforced polymer composites	157:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	8	26	theme	composites	1481:1490	arg1	temperatures					1458:1469	the heat deflection temperatures	1438:1469	the heat deflection temperatures of DSF-VE composites	1438:1490	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	0	27	theme	palm	81:84	arg1	composites					103:112	date palm seed/vinyl ester composites	76:112	date palm seed/vinyl ester composites	76:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	2	28	theme	recyclable	418:427	arg1	materials					354:362	the composite materials	340:362	the composite materials	340:362	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	2	28	theme	recyclable	418:427	arg1	reinforcements					447:460	recyclable and biodegradable reinforcements	418:460	recyclable and biodegradable reinforcements	418:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	4	29	theme	varying	778:784	arg1	loadings					794:801	varying fillers loadings	778:801	varying fillers loadings from 5% to 50%	778:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	0	30	theme	ester	97:101	arg1	composites					103:112	date palm seed/vinyl ester composites	76:112	date palm seed/vinyl ester composites	76:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	10	31	theme	engine	1904:1909	arg1	blade					1894:1898	a table fan blade	1882:1898	a table fan blade	1882:1898	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	10	31	theme	engine	1904:1909	arg1	guard					1911:1915	an engine guard	1901:1915	an engine guard for two-wheelers and self-motor guard for four wheelers	1901:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	2	32	theme	sustainable	381:391	arg1	friendly					408:415	sustainable, environmental friendly	381:415	sustainable, environmental friendly	381:415	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	6	33	theme	microscopic	1052:1062	arg1	analysis					1064:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	4	34	theme	compression	742:752	arg1	technique					763:771	conventional compression moulding technique	729:771	conventional compression moulding technique with varying fillers loadings from 5% to 50%	729:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	9	35	contain	possessed	1655:1663	arg1	composites					1644:1653	the natural filler-based (DSF-VE) composites	1610:1653	the natural filler-based (DSF-VE) composites	1610:1653	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	9	35	contain	possessed	1655:1663	arg2	stability					1673:1681	thermal stability	1665:1681	thermal stability	1665:1681	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	0	36	theme	cellulosic	10:19	arg1	loading					28:34	cellulosic filler loading	10:34	cellulosic filler loading	10:34	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	5	37	theme	deflection	911:920	arg1	temperature					922:932	heat deflection temperature	906:932	heat deflection temperature	906:932	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	6	38	theme	Scanning	1034:1041	arg1	analysis					1064:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	0	39	theme	loading	28:34	arg1	Effect					0:5	Effect	0:5	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.	0:113	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	5	40	theme	composites	992:1001	arg1	mechanical					823:832	mechanical	823:832	mechanical	823:832	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	3	41	theme	composites	630:639	arg1	properties					608:617	the properties	604:617	the properties of polymer composites	604:639	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	0	42	theme	mechanical	39:48	arg1	properties					62:71	mechanical and thermal properties	39:71	mechanical and thermal properties of date palm seed/vinyl ester composites	39:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	2	43	with	materials	354:362	arg1	friendly					408:415	sustainable, environmental friendly	381:415	sustainable, environmental friendly	381:415	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	7	44	theme	mechanical	1276:1285	arg1	hardness					1316:1323	hardness	1316:1323	hardness	1316:1323	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	44	theme	mechanical	1276:1285	arg1	40.3 MPa					1354:1361	40.3 MPa	1354:1361	40.3 MPa	1354:1361	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	44	theme	mechanical	1276:1285	arg1	properties					1287:1296	the highest mechanical properties	1264:1296	the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa	1264:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	44	theme	mechanical	1276:1285	arg1	impact					1299:1304	impact	1299:1304	impact	1299:1304	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	44	theme	mechanical	1276:1285	arg1	149 MPa					1371:1377	149 MPa	1371:1377	149 MPa	1371:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	44	theme	mechanical	1276:1285	arg1	tensile					1307:1313	tensile	1307:1313	tensile	1307:1313	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	8	45	theme	pure	1542:1545	arg1	resin					1559:1563	pure vinyl ester resin	1542:1563	pure vinyl ester resin	1542:1563	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	8	45	theme	pure	1542:1545	arg1	neat					1536:1539	the neat	1532:1539	the neat	1532:1539	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	5	46	dep	mechanical	823:832	arg1	hardness					865:872	hardness	865:872	hardness	865:872	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	5	46	dep	mechanical	823:832	arg1	impact					854:859	impact	854:859	impact	854:859	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	5	46	dep	mechanical	823:832	arg1	flexural					844:851	flexural	844:851	flexural	844:851	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	5	46	dep	mechanical	823:832	arg1	properties					967:976	properties	967:976	properties	967:976	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	5	46	dep	mechanical	823:832	arg1	tensile					835:841	tensile	835:841	tensile	835:841	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	6	47	theme	surface	1104:1110	arg1	characteristics					1112:1126	surface characteristics	1104:1126	surface characteristics	1104:1126	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	8	48	theme	ester	1553:1557	arg1	resin					1559:1563	pure vinyl ester resin	1542:1563	pure vinyl ester resin	1542:1563	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	8	48	theme	ester	1553:1557	arg1	neat					1536:1539	the neat	1532:1539	the neat	1532:1539	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	4	49	theme	5	808:808	arg1	%					809:809	%	809:809	%	809:809	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	7	50	theme	composites	1243:1252	arg1	composites					1243:1252	the DSF-VE composites	1232:1252	the DSF-VE composites	1232:1252	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	50	theme	composites	1243:1252	arg1	%					1227:1227	30 wt%	1222:1227	30 wt% of the DSF-VE composites	1222:1252	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	10	51	theme	fan	1890:1892	arg1	blade					1894:1898	a table fan blade	1882:1898	a table fan blade	1882:1898	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	10	51	theme	fan	1890:1892	arg1	guard					1911:1915	an engine guard	1901:1915	an engine guard for two-wheelers and self-motor guard for four wheelers	1901:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	2	52	theme	materials	354:362	arg1	properties					326:335	the inherent mechanical and thermal properties	290:335	the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements	290:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	9	53	theme	natural	1614:1620	arg1	composites					1644:1653	the natural filler-based (DSF-VE) composites	1610:1653	the natural filler-based (DSF-VE) composites	1610:1653	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	10	54	theme	various	1827:1833	arg1	applications					1845:1856	various potential applications	1827:1856	various potential applications	1827:1856	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	10	54	theme	various	1827:1833	arg1	fabrication					1867:1877	fabrication	1867:1877	fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers	1867:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	7	55	theme	fabricated	1404:1413	arg1	composites					1415:1424	the fabricated composites	1400:1424	the fabricated composites	1400:1424	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	6	56	theme	fractured	1132:1140	arg1	surface					1142:1148	fractured surface	1132:1148	fractured surface	1132:1148	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	3	57	theme	bio	542:544	arg1	waste					552:556	bio solid waste	542:556	bio solid waste (date seed filler)	542:575	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	3	57	theme	bio	542:544	arg1	filler					569:574	date seed filler	559:574	date seed filler	559:574	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	1	58	theme	reinforced	163:172	arg1	composites					182:191	fibre reinforced polymer composites	157:191	fibre reinforced polymer composites	157:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	6	59	theme	composites	1164:1173	arg1	characteristics					1112:1126	surface characteristics	1104:1126	surface characteristics	1104:1126	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	6	59	theme	composites	1164:1173	arg1	surface					1142:1148	fractured surface	1132:1148	fractured surface	1132:1148	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	4	60	theme	date	646:649	arg1	composites					695:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	1	61	theme	composites	182:191	arg1	applications					141:152	numerous better applications	125:152	numerous better applications of fibre reinforced polymer composites	125:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	4	62	theme	filler	656:661	arg1	composites					695:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	2	63	theme	inherent	294:301	arg1	properties					326:335	the inherent mechanical and thermal properties	290:335	the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements	290:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	9	64	theme	thermo-gravimetric	1570:1587	arg1	analysis					1589:1596	The thermo-gravimetric analysis	1566:1596	The thermo-gravimetric analysis	1566:1596	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	10	65	theme	DSF-VE	1773:1778	arg1	composites					1780:1789	the DSF-VE composites	1769:1789	the DSF-VE composites	1769:1789	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	0	66	from	Effect	0:5	arg1	properties					62:71	mechanical and thermal properties	39:71	mechanical and thermal properties of date palm seed/vinyl ester composites	39:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	8	67	theme	heat	1442:1445	arg1	temperatures					1458:1469	the heat deflection temperatures	1438:1469	the heat deflection temperatures of DSF-VE composites	1438:1490	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	2	68	dep	sustainable	381:391	arg1	environmental					394:406	environmental	394:406	environmental	394:406	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	10	69	used	utilized	1814:1821	arg2	composites					1780:1789	the DSF-VE composites	1769:1789	the DSF-VE composites	1769:1789	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	3	70	theme	date	559:562	arg1	waste					552:556	bio solid waste	542:556	bio solid waste (date seed filler)	542:575	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	3	70	theme	date	559:562	arg1	filler					569:574	date seed filler	559:574	date seed filler	559:574	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	hardness					1316:1323	hardness	1316:1323	hardness	1316:1323	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	40.3 MPa					1354:1361	40.3 MPa	1354:1361	40.3 MPa	1354:1361	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	properties					1287:1296	the highest mechanical properties	1264:1296	the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa	1264:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	impact					1299:1304	impact	1299:1304	impact	1299:1304	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	149 MPa					1371:1377	149 MPa	1371:1377	149 MPa	1371:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	71	theme	KJ/m2	1347:1351	arg1	tensile					1307:1313	tensile	1307:1313	tensile	1307:1313	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	72	theme	30 wt	1222:1226	arg1	composites					1243:1252	the DSF-VE composites	1232:1252	the DSF-VE composites	1232:1252	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	72	theme	30 wt	1222:1226	arg1	%					1227:1227	30 wt%	1222:1227	30 wt% of the DSF-VE composites	1222:1252	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	10	73	theme	potential	1835:1843	arg1	applications					1845:1856	various potential applications	1827:1856	various potential applications	1827:1856	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	10	73	theme	potential	1835:1843	arg1	fabrication					1867:1877	fabrication	1867:1877	fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers	1867:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	1	74	theme	better	134:139	arg1	applications					141:152	numerous better applications	125:152	numerous better applications of fibre reinforced polymer composites	125:191	There are numerous better applications of fibre reinforced polymer composites today, when compared with metals and alloys.
31887386	8	75	theme	DSF-VE	1474:1479	arg1	composites					1481:1490	DSF-VE composites	1474:1490	DSF-VE composites	1474:1490	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	4	76	theme	vinyl	674:678	arg1	DSF-VE					687:692	DSF-VE	687:692	DSF-VE	687:692	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	4	76	theme	vinyl	674:678	arg1	ester					680:684	reinforced vinyl ester	663:684	The date seed filler reinforced vinyl ester (DSF-VE) composites	642:704	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	0	77	theme	date	76:79	arg1	composites					103:112	date palm seed/vinyl ester composites	76:112	date palm seed/vinyl ester composites	76:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	9	78	theme	process	1735:1741	arg1	temperature					1743:1753	the polymerisation process temperature	1716:1753	the polymerisation process temperature	1716:1753	The thermo-gravimetric analysis showed that the natural filler-based (DSF-VE) composites possessed thermal stability up to 400.2 °C, which was within the polymerisation process temperature.
31887386	0	79	theme	seed/vinyl	86:95	arg1	composites					103:112	date palm seed/vinyl ester composites	76:112	date palm seed/vinyl ester composites	76:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	10	80	dep	two-wheelers	1921:1932	arg1	guard					1949:1953	guard	1949:1953	guard for four wheelers	1949:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
31887386	4	81	theme	moulding	754:761	arg1	technique					763:771	conventional compression moulding technique	729:771	conventional compression moulding technique with varying fillers loadings from 5% to 50%	729:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	0	82	theme	composites	103:112	arg1	properties					62:71	mechanical and thermal properties	39:71	mechanical and thermal properties of date palm seed/vinyl ester composites	39:112	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	5	83	theme	thermo-gravimetric	938:955	arg1	analysis					957:964	thermo-gravimetric analysis	938:964	thermo-gravimetric analysis	938:964	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	0	84	theme	filler	21:26	arg1	loading					28:34	cellulosic filler loading	10:34	cellulosic filler loading	10:34	Effect of cellulosic filler loading on mechanical and thermal properties of date palm seed/vinyl ester composites.
31887386	5	85	theme	heat	906:909	arg1	temperature					922:932	heat deflection temperature	906:932	heat deflection temperature	906:932	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	6	86	theme	electron	1043:1050	arg1	analysis					1064:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis	1034:1071	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	2	87	theme	biodegradable	433:445	arg1	materials					354:362	the composite materials	340:362	the composite materials	340:362	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	2	87	theme	biodegradable	433:445	arg1	reinforcements					447:460	recyclable and biodegradable reinforcements	418:460	recyclable and biodegradable reinforcements	418:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	6	88	dep	characteristics	1112:1126	arg1	the					1100:1102	the	1100:1102	the	1100:1102	Scanning electron microscopic analysis was carried out to analyse the surface characteristics and fractured surface of the DSF-VE composites.
31887386	4	89	theme	conventional	729:740	arg1	technique					763:771	conventional compression moulding technique	729:771	conventional compression moulding technique with varying fillers loadings from 5% to 50%	729:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	3	90	theme	current	485:491	arg1	study					493:497	this current study	480:497	this current study	480:497	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	5	91	theme	DSF-VE	985:990	arg1	composites					992:1001	the DSF-VE composites	981:1001	the DSF-VE composites	981:1001	The mechanical (tensile, flexural, impact and hardness), water absorption and thermal (heat deflection temperature and thermo-gravimetric analysis) properties of the DSF-VE composites were experimentally evaluated.
31887386	3	92	theme	polymer	622:628	arg1	composites					630:639	polymer composites	622:639	polymer composites	622:639	Consequently, in this current study, the composites were prepared by combining bio solid waste (date seed filler) and vinyl ester to enhance the properties of polymer composites.
31887386	7	93	theme	highest	1268:1274	arg1	hardness					1316:1323	hardness	1316:1323	hardness	1316:1323	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	93	theme	highest	1268:1274	arg1	40.3 MPa					1354:1361	40.3 MPa	1354:1361	40.3 MPa	1354:1361	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	93	theme	highest	1268:1274	arg1	properties					1287:1296	the highest mechanical properties	1264:1296	the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa	1264:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	93	theme	highest	1268:1274	arg1	impact					1299:1304	impact	1299:1304	impact	1299:1304	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	93	theme	highest	1268:1274	arg1	149 MPa					1371:1377	149 MPa	1371:1377	149 MPa	1371:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	93	theme	highest	1268:1274	arg1	tensile					1307:1313	tensile	1307:1313	tensile	1307:1313	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	8	94	theme	vinyl	1547:1551	arg1	resin					1559:1563	pure vinyl ester resin	1542:1563	pure vinyl ester resin	1542:1563	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	8	94	theme	vinyl	1547:1551	arg1	neat					1536:1539	the neat	1532:1539	the neat	1532:1539	Similarly, the heat deflection temperatures of DSF-VE composites increased by 58.49%, when compared with the neat, pure vinyl ester resin.
31887386	4	95	theme	50	814:815	arg1	%					809:809	%	809:809	%	809:809	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	7	96	dep	properties	1287:1296	arg1	hardness					1316:1323	hardness	1316:1323	hardness	1316:1323	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	40.3 MPa					1354:1361	40.3 MPa	1354:1361	40.3 MPa	1354:1361	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	properties					1287:1296	the highest mechanical properties	1264:1296	the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa	1264:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	51					1364:1365	51	1364:1365	51	1364:1365	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	impact					1299:1304	impact	1299:1304	impact	1299:1304	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	149 MPa					1371:1377	149 MPa	1371:1377	149 MPa	1371:1377	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	7	96	dep	properties	1287:1296	arg1	tensile					1307:1313	tensile	1307:1313	tensile	1307:1313	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	4	97	theme	fillers	786:792	arg1	loadings					794:801	varying fillers loadings	778:801	varying fillers loadings from 5% to 50%	778:816	The date seed filler reinforced vinyl ester (DSF-VE) composites were prepared by using conventional compression moulding technique with varying fillers loadings from 5% to 50%.
31887386	2	98	theme	thermal	318:324	arg1	properties					326:335	the inherent mechanical and thermal properties	290:335	the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements	290:460	Many studies have been conducted to further improve the inherent mechanical and thermal properties of the composite materials, especially with sustainable, environmental friendly, recyclable and biodegradable reinforcements.
31887386	7	99	theme	DSF-VE	1236:1241	arg1	composites					1243:1252	the DSF-VE composites	1232:1252	the DSF-VE composites	1232:1252	It was evident from the results obtained that 30 wt% of the DSF-VE composites exhibited the highest mechanical properties: impact, tensile, hardness and flexural of 17.03 KJ/m2, 40.3 MPa, 51 and 149 MPa, respectively, among the fabricated composites.
31887386	10	100	theme	blade	1894:1898	arg1	fabrication					1867:1877	fabrication	1867:1877	fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers	1867:1971	Furthermore, the DSF-VE composites have been successfully utilized for various potential applications, such as fabrication of a table fan blade, an engine guard for two-wheelers and self-motor guard for four wheelers.
30575035	8	0	theme	restructured	1139:1150	arg1	strips					1161:1166	the restructured pimiento strips	1135:1166	the restructured pimiento strips	1135:1166	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	8	0	theme	restructured	1139:1150	arg1	stable					1191:1196	stable	1191:1196	stable	1191:1196	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	6	1	theme	own	926:928	arg1	structure					930:938	its own structure	922:938	its own structure	922:938	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	4	2	theme	structural	610:619	arg1	hardness					515:522	hardness	515:522	hardness	515:522	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	4	2	theme	structural	610:619	arg1	characteristics					649:663	structural, thermal, and morphological characteristics	610:663	structural, thermal, and morphological characteristics of pimiento strips	610:682	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	10	3	theme	microstructural	1552:1566	arg1	results					1568:1574	The microstructural results	1548:1574	The microstructural results	1548:1574	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	17	4	theme	25	2697:2698	arg1	%					2699:2699	%	2699:2699	%	2699:2699	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	4	5	theme	thermal	622:628	arg1	hardness					515:522	hardness	515:522	hardness	515:522	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	4	5	theme	thermal	622:628	arg1	characteristics					649:663	structural, thermal, and morphological characteristics	610:663	structural, thermal, and morphological characteristics of pimiento strips	610:682	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	12	6	theme	alginate	2081:2088	arg1	hydrogels					2068:2076	binary composite hydrogels	2051:2076	binary composite hydrogels of alginate and guar gum	2051:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	10	7	from	evidence	1636:1643	arg1	structure					1607:1615	ellipsoidal and compact structure	1583:1615	structure	1607:1615	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	17	8	theme	pimiento	2701:2708	arg1	pulp					2710:2713	25% pimiento pulp	2697:2713	25% pimiento pulp	2697:2713	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	2	9	theme	gelling	313:319	arg1	system					321:326	Alginate-guar gelling system	299:326	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1%	299:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	1	10	theme	alginate-guar	186:198	arg1	gels					200:203	reconstituted pimiento alginate-guar gels	163:203	reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	163:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	8	11	from	temperature	1216:1226	arg1	strips					1161:1166	the restructured pimiento strips	1135:1166	the restructured pimiento strips	1135:1166	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	8	11	from	temperature	1216:1226	arg1	stable					1191:1196	stable	1191:1196	stable	1191:1196	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	2	12	theme	different	344:352	arg1	concentrations					371:384	different calcium chloride concentrations	344:384	different calcium chloride concentrations (2-8%)	344:391	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	12	theme	different	344:352	arg1	%					390:390	2-8%	387:390	2-8%	387:390	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	9	13	theme	acid	1477:1480	arg1	type					1482:1485	acid type	1477:1485	acid type	1477:1485	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	8	14	theme	pasteurization	1201:1214	arg1	temperature					1216:1226	pasteurization temperature	1201:1226	pasteurization temperature	1201:1226	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	16	15	theme	higher	2589:2594	arg1	systems					2567:2573	a restructured fruit gel systems	2542:2573	a restructured fruit gel systems several times higher than the limiting 10% pulp	2542:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	4	16	theme	pimiento	668:675	arg1	strips					677:682	pimiento strips	668:682	pimiento strips	668:682	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	12	17	dep	APPLICATIONS	1947:1958	arg1	problem					2040:2046	a challenging problem	2026:2046	a challenging problem in binary composite hydrogels of alginate and guar gum	2026:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	12	17	dep	APPLICATIONS	1947:1958	arg1	strip					1983:1987	Restructured pimiento strip	1961:1987	Restructured pimiento strip for stuffing into cocktail olives	1961:2021	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	2	18	theme	2:1	337:339	arg1	ratio					331:335	ratio 2:1	331:339	ratio 2:1 at different calcium chloride concentrations (2-8%)	331:391	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	1	19	theme	thermal	218:224	arg1	stresses					241:248	thermal and mechanical stresses	218:248	thermal and mechanical stresses	218:248	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	10	20	theme	high	1648:1651	arg1	elasticity					1653:1662	high elasticity	1648:1662	high elasticity	1648:1662	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	11	21	theme	appreciable	1864:1874	arg1	integrity					1885:1893	appreciable textural integrity	1864:1893	appreciable textural integrity during processing	1864:1911	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	9	22	theme	calcium	1256:1262	arg1	level					1264:1268	calcium level	1256:1268	calcium level	1256:1268	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	1	23	theme	mechanical	230:239	arg1	stresses					241:248	thermal and mechanical stresses	218:248	thermal and mechanical stresses	218:248	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	16	24	theme	10	2614:2615	arg1	%					2616:2616	%	2616:2616	%	2616:2616	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	7	25	theme	gel	1049:1051	arg1	shrinkage					1053:1061	the gel shrinkage	1045:1061	the gel shrinkage during thermal processing	1045:1087	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	1	26	theme	microstructural	120:134	arg1	properties					149:158	Textural, mechanical, microstructural, and thermal properties	98:158	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	98:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	11	27	theme	strips	1778:1783	arg1	rigidity					1753:1760	rigidity	1753:1760	rigidity	1753:1760	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	11	27	theme	strips	1778:1783	arg1	elasticity					1738:1747	the high elasticity	1729:1747	the high elasticity	1729:1747	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	5	28	theme	pimiento	730:737	arg1	strips					739:744	pimiento strips	730:744	pimiento strips	730:744	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	9	29	theme	pimiento	1277:1284	arg1	strip					1286:1290	the pimiento strip	1273:1290	the pimiento strip	1273:1290	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	2	30	from	ratio	331:335	arg1	system					321:326	Alginate-guar gelling system	299:326	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1%	299:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	17	31	theme	processing	2782:2791	arg1	levels					2743:2748	different levels	2733:2748	different levels of calcium chloride and thermal processing	2733:2791	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	15	32	contain	had	2356:2358	arg1	it					2348:2349	it	2348:2349	it	2348:2349	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	15	32	contain	had	2356:2358	arg2	shrinkage					2378:2386	shrinkage	2378:2386	shrinkage	2378:2386	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	15	32	contain	had	2356:2358	arg2	syneresis					2364:2372	low syneresis	2360:2372	low syneresis	2360:2372	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	12	33	theme	composite	2058:2066	arg1	hydrogels					2068:2076	binary composite hydrogels	2051:2076	binary composite hydrogels of alginate and guar gum	2051:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	9	34	dep	transform	1360:1368	arg1	infrared					1370:1377	infrared	1370:1377	transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions	1360:1545	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	0	35	theme	alginate-guar	78:90	arg1	gels					92:95	pimiento alginate-guar gels	69:95	pimiento alginate-guar gels	69:95	Textural, mechanical, and microstructural properties of restructured pimiento alginate-guar gels.
30575035	9	36	theme	transition	1299:1308	arg1	temperature					1310:1320	glass transition temperature	1293:1320	glass transition temperature	1293:1320	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	15	37	theme	low	2360:2362	arg1	syneresis					2364:2372	low syneresis	2360:2372	low syneresis	2360:2372	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	11	38	with	stresses	1850:1857	arg1	integrity					1885:1893	appreciable textural integrity	1864:1893	appreciable textural integrity during processing	1864:1911	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	1	39	theme	reconstituted	163:175	arg1	gels					200:203	reconstituted pimiento alginate-guar gels	163:203	reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	163:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	15	40	dep	syneresis	2364:2372	arg1	fall					2408:2411	fall	2408:2411	not fall out of the olive hole during storage	2404:2448	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	9	41	dep	98°C.	1346:1350	arg1	to					1343:1344	to	1343:1344	to	1343:1344	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	14	42	theme	olive	2328:2332	arg1	hole					2320:2323	the pit hole	2312:2323	the pit hole of olive	2312:2332	Furthermore, it had to withstand to pasteurization process and be flexible to bend like a hairpin into the pit hole of olive.
30575035	5	43	theme	calcium	771:777	arg1	levels					779:784	higher calcium levels	764:784	higher calcium levels	764:784	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	16	44	theme	several	2575:2581	arg1	times					2583:2587	several times	2575:2587	several times	2575:2587	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	9	45	contain	has	1495:1497	arg2	effect					1511:1516	significant effect	1499:1516	significant effect	1499:1516	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	9	45	contain	has	1495:1497	arg1	type					1482:1485	acid type	1477:1485	acid type	1477:1485	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	10	46	theme	pimiento	1680:1687	arg1	gels					1689:1692	pimiento gels	1680:1692	pimiento gels	1680:1692	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	14	47	theme	pasteurization	2245:2258	arg1	process					2260:2266	pasteurization process	2245:2266	pasteurization process	2245:2266	Furthermore, it had to withstand to pasteurization process and be flexible to bend like a hairpin into the pit hole of olive.
30575035	10	48	theme	rigidity	1668:1675	arg1	evidence					1636:1643	an evidence	1633:1643	an evidence of high elasticity and rigidity of pimiento gels	1633:1692	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	0	49	theme	Textural	0:7	arg1	properties					42:51	Textural, mechanical, and microstructural properties	0:51	Textural, mechanical, and microstructural properties of	0:54	Textural, mechanical, and microstructural properties of restructured pimiento alginate-guar gels.
30575035	12	50	theme	Restructured	1961:1972	arg1	problem					2040:2046	a challenging problem	2026:2046	a challenging problem in binary composite hydrogels of alginate and guar gum	2026:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	12	50	theme	Restructured	1961:1972	arg1	strip					1983:1987	Restructured pimiento strip	1961:1987	Restructured pimiento strip for stuffing into cocktail olives	1961:2021	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	1	51	theme	Textural	98:105	arg1	properties					149:158	Textural, mechanical, microstructural, and thermal properties	98:158	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	98:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	11	52	theme	high	1733:1736	arg1	elasticity					1738:1747	the high elasticity	1729:1747	the high elasticity	1729:1747	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	2	53	theme	1	454:454	arg1	%					455:455	%	455:455	%	455:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	0	54	theme	mechanical	10:19	arg1	properties					42:51	Textural, mechanical, and microstructural properties	0:51	Textural, mechanical, and microstructural properties of	0:54	Textural, mechanical, and microstructural properties of restructured pimiento alginate-guar gels.
30575035	16	55	from	advantageous	2518:2529	arg1	point					2463:2467	the view point	2454:2467	the view point of marketing and nutritional aspects	2454:2504	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	9	56	from	conditions	1451:1460	arg1	stable					1466:1471	stable	1466:1471	stable	1466:1471	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	9	56	from	conditions	1451:1460	arg1	structure					1431:1439	the gel network structure	1415:1439	the gel network structure at acidic conditions	1415:1460	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	16	57	theme	aspects	2498:2504	arg1	point					2463:2467	the view point	2454:2467	the view point of marketing and nutritional aspects	2454:2504	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	1	58	theme	mechanical	108:117	arg1	properties					149:158	Textural, mechanical, microstructural, and thermal properties	98:158	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	98:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	1	59	theme	thermal	141:147	arg1	properties					149:158	Textural, mechanical, microstructural, and thermal properties	98:158	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	98:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	17	60	theme	calcium	2753:2759	arg1	chloride					2761:2768	calcium chloride	2753:2768	calcium chloride	2753:2768	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	4	61	theme	mechanical	587:596	arg1	hardness					515:522	hardness	515:522	hardness	515:522	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	4	61	theme	mechanical	587:596	arg1	properties					598:607	mechanical properties	587:607	adhesiveness as well as mechanical properties	563:607	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	2	62	theme	lactic	442:447	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	3	63	theme	Textural	473:480	arg1	analysis					490:497	Textural profile analysis	473:497	Textural profile analysis	473:497	Textural profile analysis parameters viz.
30575035	17	64	theme	chloride	2761:2768	arg1	levels					2743:2748	different levels	2733:2748	different levels of calcium chloride and thermal processing	2733:2791	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	12	65	from	problem	2040:2046	arg1	hydrogels					2068:2076	binary composite hydrogels	2051:2076	binary composite hydrogels of alginate and guar gum	2051:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	2	66	from	conditions	410:419	arg1	system					321:326	Alginate-guar gelling system	299:326	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1%	299:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	16	67	from	point	2463:2467	arg1	advantageous					2518:2529	advantageous	2518:2529	advantageous	2518:2529	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	6	68	theme	high	881:884	arg1	content					891:897	high pulp content	881:897	high pulp content (25%)	881:903	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	6	68	theme	high	881:884	arg1	%					902:902	25%	900:902	25%	900:902	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	16	69	theme	weak	2638:2641	arg1	structure					2643:2651	its weak structure	2634:2651	its weak structure	2634:2651	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	17	70	contain	containing	2686:2695	arg1	systems					2678:2684	alginate-gel systems	2665:2684	alginate-gel systems containing 25% pimiento pulp	2665:2713	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	17	70	contain	containing	2686:2695	arg2	pulp					2710:2713	25% pimiento pulp	2697:2713	25% pimiento pulp	2697:2713	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	10	71	theme	ellipsoidal	1583:1593	arg1	structure					1607:1615	ellipsoidal and compact structure	1583:1615	structure	1607:1615	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	3	72	theme	profile	482:488	arg1	analysis					490:497	Textural profile analysis	473:497	Textural profile analysis	473:497	Textural profile analysis parameters viz.
30575035	2	73	theme	varying	397:403	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	73	theme	varying	397:403	arg1	conditions					410:419	varying acid conditions	397:419	varying acid conditions including citric and lactic acid 1%	397:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	9	74	theme	gel	1419:1421	arg1	stable					1466:1471	stable	1466:1471	stable	1466:1471	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	9	74	theme	gel	1419:1421	arg1	structure					1431:1439	the gel network structure	1415:1439	the gel network structure at acidic conditions	1415:1460	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	16	75	theme	restructured	2544:2555	arg1	systems					2567:2573	a restructured fruit gel systems	2542:2573	a restructured fruit gel systems several times higher than the limiting 10% pulp	2542:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	4	76	theme	morphological	635:647	arg1	hardness					515:522	hardness	515:522	hardness	515:522	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	4	76	theme	morphological	635:647	arg1	characteristics					649:663	structural, thermal, and morphological characteristics	610:663	structural, thermal, and morphological characteristics of pimiento strips	610:682	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	16	77	theme	marketing	2472:2480	arg1	aspects					2498:2504	marketing and nutritional aspects	2472:2504	marketing and nutritional aspects	2472:2504	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	12	78	theme	gum	2099:2101	arg1	hydrogels					2068:2076	binary composite hydrogels	2051:2076	binary composite hydrogels of alginate and guar gum	2051:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	16	79	theme	view	2458:2461	arg1	point					2463:2467	the view point	2454:2467	the view point of marketing and nutritional aspects	2454:2504	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	2	80	from	concentrations	371:384	arg1	ratio					331:335	ratio 2:1	331:339	ratio 2:1 at different calcium chloride concentrations (2-8%)	331:391	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	80	from	concentrations	371:384	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	80	from	concentrations	371:384	arg1	conditions					410:419	varying acid conditions	397:419	varying acid conditions including citric and lactic acid 1%	397:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	5	81	theme	Gel	699:701	arg1	strength					703:710	Gel strength	699:710	Gel strength	699:710	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	9	82	theme	acidic	1444:1449	arg1	conditions					1451:1460	acidic conditions	1444:1460	acidic conditions	1444:1460	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	8	83	theme	pimiento	1152:1159	arg1	strips					1161:1166	the restructured pimiento strips	1135:1166	the restructured pimiento strips	1135:1166	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	8	83	theme	pimiento	1152:1159	arg1	stable					1191:1196	stable	1191:1196	stable	1191:1196	Therefore, the restructured pimiento strips were completely thermo stable at pasteurization temperature.
30575035	17	84	theme	%	2699:2699	arg1	pulp					2710:2713	25% pimiento pulp	2697:2713	25% pimiento pulp	2697:2713	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	10	85	theme	compact	1599:1605	arg1	structure					1607:1615	ellipsoidal and compact structure	1583:1615	structure	1607:1615	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	1	86	theme	pimiento	177:184	arg1	gels					200:203	reconstituted pimiento alginate-guar gels	163:203	reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	163:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	6	87	theme	gel	837:839	arg1	system					841:846	the gel system	833:846	the gel system	833:846	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	2	88	theme	calcium	354:360	arg1	concentrations					371:384	different calcium chloride concentrations	344:384	different calcium chloride concentrations (2-8%)	344:391	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	88	theme	calcium	354:360	arg1	%					390:390	2-8%	387:390	2-8%	387:390	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	1	89	theme	gels	200:203	arg1	properties					149:158	Textural, mechanical, microstructural, and thermal properties	98:158	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process	98:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	12	90	theme	challenging	2028:2038	arg1	problem					2040:2046	a challenging problem	2026:2046	a challenging problem in binary composite hydrogels of alginate and guar gum	2026:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	12	90	theme	challenging	2028:2038	arg1	strip					1983:1987	Restructured pimiento strip	1961:1987	Restructured pimiento strip for stuffing into cocktail olives	1961:2021	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	11	91	theme	mechanical	1839:1848	arg1	pasteurization					1921:1934	pasteurization	1921:1934	pasteurization	1921:1934	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	11	91	theme	mechanical	1839:1848	arg1	stresses					1850:1857	thermal and mechanical stresses	1827:1857	thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization	1827:1934	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	5	92	theme	strips	739:744	arg1	elasticity					716:725	elasticity	716:725	elasticity	716:725	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	5	92	theme	strips	739:744	arg1	strength					703:710	Gel strength	699:710	Gel strength	699:710	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	16	93	theme	limiting	2605:2612	arg1	pulp					2618:2621	the limiting 10% pulp	2601:2621	the limiting 10% pulp	2601:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	10	94	theme	elasticity	1653:1662	arg1	evidence					1636:1643	an evidence	1633:1643	an evidence of high elasticity and rigidity of pimiento gels	1633:1692	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	11	95	theme	thermal	1827:1833	arg1	pasteurization					1921:1934	pasteurization	1921:1934	pasteurization	1921:1934	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	11	95	theme	thermal	1827:1833	arg1	stresses					1850:1857	thermal and mechanical stresses	1827:1857	thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization	1827:1934	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	12	96	theme	cocktail	2007:2014	arg1	olives					2016:2021	cocktail olives	2007:2021	cocktail olives	2007:2021	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	16	97	theme	%	2616:2616	arg1	pulp					2618:2621	the limiting 10% pulp	2601:2621	the limiting 10% pulp	2601:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	16	98	theme	gel	2563:2565	arg1	systems					2567:2573	a restructured fruit gel systems	2542:2573	a restructured fruit gel systems several times higher than the limiting 10% pulp	2542:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	12	99	theme	binary	2051:2056	arg1	hydrogels					2068:2076	binary composite hydrogels	2051:2076	binary composite hydrogels of alginate and guar gum	2051:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	4	100	theme	strips	677:682	arg1	hardness					515:522	hardness	515:522	hardness	515:522	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	4	100	theme	strips	677:682	arg1	characteristics					649:663	structural, thermal, and morphological characteristics	610:663	structural, thermal, and morphological characteristics of pimiento strips	610:682	hardness, springiness, gumminess, cohesiveness, adhesiveness as well as mechanical properties, structural, thermal, and morphological characteristics of pimiento strips were examined.
30575035	11	101	theme	textural	1876:1883	arg1	integrity					1885:1893	appreciable textural integrity	1864:1893	appreciable textural integrity during processing	1864:1911	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	9	102	theme	significant	1499:1509	arg1	effect					1511:1516	significant effect	1499:1516	significant effect	1499:1516	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	7	103	theme	thermal	1070:1076	arg1	processing					1078:1087	thermal processing	1070:1087	thermal processing	1070:1087	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	7	104	from	Increase	941:948	arg1	hardness					953:960	hardness	953:960	hardness	953:960	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	7	104	from	Increase	941:948	arg1	springiness					979:989	springiness	979:989	springiness	979:989	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	2	105	theme	Alginate-guar	299:311	arg1	system					321:326	Alginate-guar gelling system	299:326	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1%	299:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	17	106	theme	alginate-gel	2665:2676	arg1	systems					2678:2684	alginate-gel systems	2665:2684	alginate-gel systems containing 25% pimiento pulp	2665:2713	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	0	107	theme	pimiento	69:76	arg1	gels					92:95	pimiento alginate-guar gels	69:95	pimiento alginate-guar gels	69:95	Textural, mechanical, and microstructural properties of restructured pimiento alginate-guar gels.
30575035	7	108	theme	elasticity	1006:1015	arg1	loss					998:1001	loss	998:1001	loss of elasticity, which may be attributed to the gel shrinkage during thermal processing	998:1087	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	9	109	theme	chemical	1525:1532	arg1	interactions					1534:1545	the chemical interactions	1521:1545	the chemical interactions	1521:1545	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	16	110	theme	fruit	2557:2561	arg1	systems					2567:2573	a restructured fruit gel systems	2542:2573	a restructured fruit gel systems several times higher than the limiting 10% pulp	2542:2621	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	9	111	dep	Fourier	1352:1358	arg1	transform					1360:1368	transform	1360:1368	transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions	1360:1545	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	7	112	from	reduction	966:974	arg1	hardness					953:960	hardness	953:960	hardness	953:960	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	7	112	from	reduction	966:974	arg1	springiness					979:989	springiness	979:989	springiness	979:989	Increase in hardness and reduction in springiness showed loss of elasticity, which may be attributed to the gel shrinkage during thermal processing, making it more compact and dense.
30575035	11	113	theme	pimiento	1769:1776	arg1	strips					1778:1783	the pimiento strips	1765:1783	the pimiento strips	1765:1783	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	9	114	theme	glass	1293:1297	arg1	temperature					1310:1320	glass transition temperature	1293:1320	glass transition temperature	1293:1320	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	5	115	theme	higher	764:769	arg1	levels					779:784	higher calcium levels	764:784	higher calcium levels	764:784	Gel strength and elasticity of pimiento strips were increased at higher calcium levels.
30575035	11	116	theme	ability	1795:1801	arg1	rigidity					1753:1760	rigidity	1753:1760	rigidity	1753:1760	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	11	116	theme	ability	1795:1801	arg1	elasticity					1738:1747	the high elasticity	1729:1747	the high elasticity	1729:1747	These results completely approved the high elasticity and rigidity of the pimiento strips and their ability to sustain successfully thermal and mechanical stresses with appreciable textural integrity during processing such as pasteurization.
30575035	14	117	theme	pit	2316:2318	arg1	hole					2320:2323	the pit hole	2312:2323	the pit hole of olive	2312:2332	Furthermore, it had to withstand to pasteurization process and be flexible to bend like a hairpin into the pit hole of olive.
30575035	12	118	theme	pimiento	1974:1981	arg1	problem					2040:2046	a challenging problem	2026:2046	a challenging problem in binary composite hydrogels of alginate and guar gum	2026:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	12	118	theme	pimiento	1974:1981	arg1	strip					1983:1987	Restructured pimiento strip	1961:1987	Restructured pimiento strip for stuffing into cocktail olives	1961:2021	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	17	119	theme	different	2733:2741	arg1	levels					2743:2748	different levels	2733:2748	different levels of calcium chloride and thermal processing	2733:2791	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	2	120	theme	acid	405:408	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	120	theme	acid	405:408	arg1	conditions					410:419	varying acid conditions	397:419	varying acid conditions including citric and lactic acid 1%	397:455	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	121	theme	%	455:455	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	0	122	theme	microstructural	26:40	arg1	properties					42:51	Textural, mechanical, and microstructural properties	0:51	Textural, mechanical, and microstructural properties of	0:54	Textural, mechanical, and microstructural properties of restructured pimiento alginate-guar gels.
30575035	12	123	theme	PRACTICAL	1937:1945	arg1	APPLICATIONS					1947:1958	PRACTICAL APPLICATIONS	1937:1958	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.	1937:2102	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	16	124	theme	nutritional	2486:2496	arg1	aspects					2498:2504	marketing and nutritional aspects	2472:2504	marketing and nutritional aspects	2472:2504	In the view point of marketing and nutritional aspects, it is more advantageous to develop a restructured fruit gel systems several times higher than the limiting 10% pulp because of its weak structure.
30575035	2	125	theme	citric	431:436	arg1	acid					449:452	citric and lactic acid	431:452	acid	449:452	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	1	126	theme	pasteurization	257:270	arg1	process					272:278	pasteurization process	257:278	pasteurization process	257:278	Textural, mechanical, microstructural, and thermal properties of reconstituted pimiento alginate-guar gels subjected to thermal and mechanical stresses during pasteurization process were investigated.
30575035	10	127	theme	gels	1689:1692	arg1	rigidity					1668:1675	rigidity	1668:1675	rigidity of pimiento gels	1668:1692	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	10	127	theme	gels	1689:1692	arg1	elasticity					1653:1662	high elasticity	1648:1662	high elasticity	1648:1662	The microstructural results showed ellipsoidal and compact structure in which can be an evidence of high elasticity and rigidity of pimiento gels.
30575035	9	128	theme	spectroscopy	1379:1390	arg1	studies					1392:1398	spectroscopy studies	1379:1398	spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions	1379:1545	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	6	129	theme	pulp	886:889	arg1	content					891:897	high pulp content	881:897	high pulp content (25%)	881:903	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	6	129	theme	pulp	886:889	arg1	%					902:902	25%	900:902	25%	900:902	Fracturability was decreased clearly revealed the gel system, regain its strength in spite of high pulp content (25%) and can maintain its own structure.
30575035	15	130	theme	olive	2424:2428	arg1	hole					2430:2433	the olive hole	2420:2433	the olive hole during storage	2420:2448	In addition, it also had low syneresis and shrinkage that the strips not fall out of the olive hole during storage.
30575035	17	131	theme	thermal	2774:2780	arg1	processing					2782:2791	thermal processing	2774:2791	thermal processing	2774:2791	Therefore, alginate-gel systems containing 25% pimiento pulp were evaluated at different levels of calcium chloride and thermal processing.
30575035	2	132	theme	chloride	362:369	arg1	concentrations					371:384	different calcium chloride concentrations	344:384	different calcium chloride concentrations (2-8%)	344:391	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	2	132	theme	chloride	362:369	arg1	%					390:390	2-8%	387:390	2-8%	387:390	Alginate-guar gelling system at ratio 2:1 at different calcium chloride concentrations (2-8%) and varying acid conditions including citric and lactic acid 1% were evaluated.
30575035	12	133	theme	guar	2094:2097	arg1	gum					2099:2101	guar gum	2094:2101	guar gum	2094:2101	PRACTICAL APPLICATIONS: Restructured pimiento strip for stuffing into cocktail olives is a challenging problem in binary composite hydrogels of alginate and guar gum.
30575035	9	134	theme	network	1423:1429	arg1	stable					1466:1471	stable	1466:1471	stable	1466:1471	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
30575035	9	134	theme	network	1423:1429	arg1	structure					1431:1439	the gel network structure	1415:1439	the gel network structure at acidic conditions	1415:1460	By increasing alginate and calcium level in the pimiento strip, glass transition temperature was reduced from 112 to 98°C. Fourier transform infrared spectroscopy studies confirmed that the gel network structure at acidic conditions was stable and acid type did not has significant effect on the chemical interactions.
29909634	2	0	theme	SeLe	446:449	arg1	status					451:456	the mother's SeLe status	433:456	the mother's SeLe status	433:456	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	0	1	from	Oligosaccharides	11:26	arg1	Colostrum					31:39	Colostrum	31:39	Colostrum	31:39	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	0	1	from	Oligosaccharides	11:26	arg1	Milk					52:55	Mature Milk	45:55	Mature Milk	45:55	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	1	2	theme	human	140:144	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	1	3	theme	lactation	220:228	arg1	period					230:235	a 20-wk lactation period	212:235	a 20-wk lactation period	212:235	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	0	4	theme	Mothers	68:74	arg1	Colostrum					31:39	Colostrum	31:39	Colostrum	31:39	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	0	4	theme	Mothers	68:74	arg1	Milk					52:55	Mature Milk	45:55	Mature Milk	45:55	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	4	5	theme	nonsecretor	800:810	arg1	group					812:816	the nonsecretor group	796:816	the nonsecretor group	796:816	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	1	6	theme	milk	146:149	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	0	7	theme	Positive	83:90	arg1	Subgroups					101:109	Lewis Positive Secretor Subgroups	77:109	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	3	8	theme	HMO	534:536	arg1	concentrations					538:551	total acidic and neutral HMO concentrations	509:551	total acidic and neutral HMO concentrations in Chinese human milk	509:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	7	9	theme	human	1090:1094	arg1	milk					1096:1099	Dutch human milk	1084:1099	Dutch human milk	1084:1099	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	4	10	theme	fucosylated	689:699	arg1	concentrations					705:718	total neutral fucosylated HMO concentrations	675:718	total neutral fucosylated HMO concentrations in Chinese human milk	675:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	0	11	theme	Lewis	77:81	arg1	Subgroups					101:109	Lewis Positive Secretor Subgroups	77:109	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	1	12	from	variability	125:135	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	2	13	theme	total	316:320	arg1	concentrations					326:339	total HMO concentrations	316:339	total HMO concentrations in Chinese human milk	316:361	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	5	14	dep	concentrations	869:882	arg1	the					822:824	the	822:824	the	822:824	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	5	14	dep	concentrations	869:882	arg1	basis					826:830	basis	826:830	basis	826:830	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	1	15	theme	HMO	238:240	arg1	profiles					242:249	HMO profiles	238:249	HMO profiles of 30 mothers	238:263	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	4	16	gly	fucosylated	689:699	arg1	concentrations					705:718	total neutral fucosylated HMO concentrations	675:718	total neutral fucosylated HMO concentrations in Chinese human milk	675:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	6	17	theme	Se+Le+	1015:1020	arg1	subgroups					1022:1030	Se+Le+ subgroups	1015:1030	Se+Le+ subgroups	1015:1030	HMOs that differed in level between Se+Le+ subgroups were 2'FL, DF-L, LNFP I, and F-LNO.
29909634	4	18	from	concentrations	705:718	arg1	milk					737:740	Chinese human milk	723:740	Chinese human milk	723:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	4	19	theme	human	731:735	arg1	milk					737:740	Chinese human milk	723:740	Chinese human milk	723:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	2	20	theme	Chinese	344:350	arg1	milk					358:361	Chinese human milk	344:361	Chinese human milk	344:361	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	1	21	theme	oligosaccharide	151:165	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	0	22	theme	Secretor	92:99	arg1	Subgroups					101:109	Lewis Positive Secretor Subgroups	77:109	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	4	23	theme	Chinese	723:729	arg1	milk					737:740	Chinese human milk	723:740	Chinese human milk	723:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	2	24	theme	human	352:356	arg1	milk					358:361	Chinese human milk	344:361	Chinese human milk	344:361	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	0	25	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	5	26	theme	Se+Le+	931:936	arg1	group					938:942	the Se+Le+ group	927:942	the Se+Le+ group	927:942	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	4	27	theme	neutral	681:687	arg1	concentrations					705:718	total neutral fucosylated HMO concentrations	675:718	total neutral fucosylated HMO concentrations in Chinese human milk	675:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	1	28	theme	HMO	168:170	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	0	29	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	2	30	from	concentrations	326:339	arg1	milk					358:361	Chinese human milk	344:361	Chinese human milk	344:361	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	3	31	theme	neutral	526:532	arg1	concentrations					538:551	total acidic and neutral HMO concentrations	509:551	total acidic and neutral HMO concentrations in Chinese human milk	509:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	2	32	theme	lactation	400:408	arg1	period					410:415	a 20-wk lactation period	392:415	a 20-wk lactation period	392:415	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	4	33	theme	total	675:679	arg1	concentrations					705:718	total neutral fucosylated HMO concentrations	675:718	total neutral fucosylated HMO concentrations in Chinese human milk	675:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	3	34	theme	total	509:513	arg1	concentrations					538:551	total acidic and neutral HMO concentrations	509:551	total acidic and neutral HMO concentrations in Chinese human milk	509:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	3	35	from	concentrations	538:551	arg1	milk					570:573	Chinese human milk	556:573	Chinese human milk	556:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	2	36	theme	20-wk	394:398	arg1	period					410:415	a 20-wk lactation period	392:415	a 20-wk lactation period	392:415	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	5	37	theme	HMO	907:909	arg1	profiles					911:918	HMO profiles	907:918	HMO profiles within the Se+Le+ group	907:942	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	5	38	theme	Chinese	887:893	arg1	milk					901:904	Chinese human milk	887:904	Chinese human milk	887:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	3	39	theme	acidic	515:520	arg1	concentrations					538:551	total acidic and neutral HMO concentrations	509:551	total acidic and neutral HMO concentrations in Chinese human milk	509:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	1	40	theme	mothers	257:263	arg1	profiles					242:249	HMO profiles	238:249	HMO profiles of 30 mothers	238:263	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	7	41	theme	HMO	1068:1070	arg1	profiles					1072:1079	HMO profiles	1068:1079	HMO profiles in Dutch human milk	1068:1099	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	7	42	from	profiles	1072:1079	arg1	milk					1096:1099	Dutch human milk	1084:1099	Dutch human milk	1084:1099	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	1	43	theme	Chinese	188:194	arg1	milk					202:205	Chinese human milk	188:205	Chinese human milk	188:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	5	44	gly	fucosylated	853:863	arg1	concentrations					869:882	the total neutral fucosylated HMO concentrations	835:882	the total neutral fucosylated HMO concentrations in Chinese human milk	835:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	3	45	theme	Chinese	556:562	arg1	milk					570:573	Chinese human milk	556:573	Chinese human milk	556:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	1	46	theme	human	196:200	arg1	milk					202:205	Chinese human milk	188:205	Chinese human milk	188:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	2	47	theme	individual	473:482	arg1	variations					484:493	individual variations	473:493	individual variations	473:493	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	5	48	theme	total	839:843	arg1	concentrations					869:882	the total neutral fucosylated HMO concentrations	835:882	the total neutral fucosylated HMO concentrations in Chinese human milk	835:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	0	49	theme	Mature	45:50	arg1	Milk					52:55	Mature Milk	45:55	Mature Milk	45:55	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	7	50	theme	subgroup	1120:1127	arg1	division					1129:1136	Se+Le+ subgroup division	1113:1136	Se+Le+ subgroup division	1113:1136	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	4	51	theme	secretor	765:772	arg1	groups					774:779	the two secretor groups	757:779	the two secretor groups	757:779	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	7	52	theme	Dutch	1084:1088	arg1	milk					1096:1099	Dutch human milk	1084:1099	Dutch human milk	1084:1099	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	5	53	theme	neutral	845:851	arg1	concentrations					869:882	the total neutral fucosylated HMO concentrations	835:882	the total neutral fucosylated HMO concentrations in Chinese human milk	835:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	5	54	theme	fucosylated	853:863	arg1	concentrations					869:882	the total neutral fucosylated HMO concentrations	835:882	the total neutral fucosylated HMO concentrations in Chinese human milk	835:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	4	55	theme	HMO	701:703	arg1	concentrations					705:718	total neutral fucosylated HMO concentrations	675:718	total neutral fucosylated HMO concentrations in Chinese human milk	675:740	Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups as compared to the nonsecretor group.
29909634	1	56	theme	milk	202:205	arg1	composition					173:183	human milk oligosaccharide (HMO) composition	140:183	human milk oligosaccharide (HMO) composition of Chinese human milk	140:205	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29909634	0	57	dep	Oligosaccharides	11:26	arg1	Subgroups					101:109	Lewis Positive Secretor Subgroups	77:109	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.	0:110	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	5	58	theme	HMO	865:867	arg1	concentrations					869:882	the total neutral fucosylated HMO concentrations	835:882	the total neutral fucosylated HMO concentrations in Chinese human milk	835:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	5	59	from	concentrations	869:882	arg1	milk					901:904	Chinese human milk	887:904	Chinese human milk	887:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	0	60	theme	Chinese	60:66	arg1	Mothers					68:74	Chinese Mothers	60:74	Chinese Mothers	60:74	Human Milk Oligosaccharides in Colostrum and Mature Milk of Chinese Mothers: Lewis Positive Secretor Subgroups.
29909634	3	61	theme	human	564:568	arg1	milk					570:573	Chinese human milk	556:573	Chinese human milk	556:573	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	5	62	theme	human	895:899	arg1	milk					901:904	Chinese human milk	887:904	Chinese human milk	887:904	On the basis of the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided into two subgroups.
29909634	7	63	theme	driving	1172:1178	arg1	force					1180:1184	the driving force	1168:1184	the driving force	1168:1184	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	2	64	theme	status	451:456	arg1	independent					418:428	independent	418:428	independent	418:428	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	3	65	theme	SeLe	641:644	arg1	status					646:651	their mother's SeLe status	626:651	their mother's SeLe status	626:651	In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother's SeLe status.
29909634	2	66	theme	HMO	322:324	arg1	concentrations					326:339	total HMO concentrations	316:339	total HMO concentrations in Chinese human milk	316:361	This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother's SeLe status, although with individual variations.
29909634	7	67	theme	Se+Le+	1113:1118	arg1	division					1129:1136	Se+Le+ subgroup division	1113:1136	Se+Le+ subgroup division	1113:1136	HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2'FL, LNT, and F-LNO as the driving force.
29909634	1	68	theme	20-wk	214:218	arg1	period					230:235	a 20-wk lactation period	212:235	a 20-wk lactation period	212:235	To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF.
29767310	0	0	theme	alginate	81:88	arg1	coagulants					100:109	modified sodium alginate composite coagulants	65:109	modified sodium alginate composite coagulants	65:109	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	4	1	theme	better	662:667	arg1	performance					681:691	better coagulation performance	662:691	better coagulation performance	662:691	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	6	2	theme	optimal	1175:1181	arg1	condition					1195:1203	the optimal coagulation condition	1171:1203	the optimal coagulation condition	1171:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	0	3	theme	sodium	74:79	arg1	coagulants					100:109	modified sodium alginate composite coagulants	65:109	modified sodium alginate composite coagulants	65:109	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	5	4	theme	larger	836:841	arg1	flocs					854:858	larger and looser flocs	836:858	larger and looser flocs	836:858	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	9	5	theme	nanoparticle	1369:1380	arg1	recycle					1382:1388	silver nanoparticle recycle	1362:1388	silver nanoparticle recycle	1362:1388	It gives us a possible way for silver nanoparticle recycle.
29767310	6	6	theme	those	1144:1148	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	6	theme	those	1144:1148	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	6	theme	those	1144:1148	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	6	theme	those	1144:1148	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	Ref-s					1041:1045	Ref-s	1041:1045	Ref-s	1041:1045	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	7	theme	MSA	1114:1116	arg1	Rif-r					1096:1100	Rif-r	1096:1100	Rif-r	1096:1100	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	5	8	theme	SA	883:884	arg1	coagulant					896:904	SA composite coagulant	883:904	SA composite coagulant	883:904	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	4	9	from	condition	768:776	arg1	%					751:751	97 and 59%	742:751	97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L)	742:812	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	0	10	theme	composite	90:98	arg1	coagulants					100:109	modified sodium alginate composite coagulants	65:109	modified sodium alginate composite coagulants	65:109	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	1	11	theme	coagulation-ultrafiltration	301:327	arg1	process					329:335	coagulation-ultrafiltration process	301:335	coagulation-ultrafiltration process	301:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	5	12	theme	composite	886:894	arg1	coagulant					896:904	SA composite coagulant	883:904	SA composite coagulant	883:904	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	0	13	theme	floc	112:115	arg1	properties					117:126	floc properties	112:126	floc properties	112:126	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	6	14	theme	fouling	1076:1082	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	14	theme	fouling	1076:1082	arg1	Rif-r					1096:1100	Rif-r	1096:1100	Rif-r	1096:1100	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	14	theme	fouling	1076:1082	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	14	theme	fouling	1076:1082	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	2	15	theme	membrane	362:369	arg1	fouling					371:377	membrane fouling	362:377	membrane fouling	362:377	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	2	16	theme	laser	454:458	arg1	instrument					472:481	a laser diffraction instrument	452:481	a laser diffraction instrument	452:481	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	6	17	theme	internal	1067:1074	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	17	theme	internal	1067:1074	arg1	Rif-r					1096:1100	Rif-r	1096:1100	Rif-r	1096:1100	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	17	theme	internal	1067:1074	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	17	theme	internal	1067:1074	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	2	18	theme	diffraction	460:470	arg1	instrument					472:481	a laser diffraction instrument	452:481	a laser diffraction instrument	452:481	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	1	19	used	used	239:242	arg2	alginate					198:205	a modified sodium alginate	180:205	a modified sodium alginate (MSA) composited with TiCl4	180:233	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	1	19	used	used	239:242	arg2	MSA					208:210	MSA	208:210	MSA	208:210	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	4	20	theme	dosage = 12 mg	790:803	arg1	TiCl4/L					805:811	dosage = 12 mg TiCl4/L	790:811	dosage = 12 mg TiCl4/L	790:811	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	6	21	theme	reversible	1056:1065	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	21	theme	reversible	1056:1065	arg1	Rif-r					1096:1100	Rif-r	1096:1100	Rif-r	1096:1100	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	21	theme	reversible	1056:1065	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	21	theme	reversible	1056:1065	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	9	22	theme	possible	1345:1352	arg1	way					1354:1356	a possible way	1343:1356	a possible way for silver nanoparticle recycle	1343:1388	It gives us a possible way for silver nanoparticle recycle.
29767310	6	23	from	condition	1195:1203	arg1	TiCl4					1162:1166	TiCl4	1162:1166	TiCl4 at the optimal coagulation condition	1162:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	2	24	dep	coagulants	406:415	arg1	MSA					426:428	MSA	426:428	MSA	426:428	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	2	24	dep	coagulants	406:415	arg1	TiCl4					418:422	TiCl4	418:422	TiCl4	418:422	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	7	25	theme	adopted	1219:1225	arg1	AgCl-TiO2					1227:1235	the adopted AgCl-TiO2	1215:1235	the adopted AgCl-TiO2	1215:1235	Besides, the adopted AgCl-TiO2 could be recycled from AgNP-containing flocs.
29767310	2	26	theme	floc	342:345	arg1	properties					347:356	floc properties	342:356	floc properties	342:356	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	0	27	theme	silver	15:20	arg1	nanoparticle					22:33	silver nanoparticle	15:33	silver nanoparticle	15:33	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	3	28	theme	flocs	553:557	arg1	recycle					522:528	The recycle	518:528	The recycle of the AgNP-containing flocs	518:557	The recycle of the AgNP-containing flocs was evaluated by XRD and photocatalytic experiments.
29767310	7	29	theme	AgNP-containing	1260:1274	arg1	flocs					1276:1280	AgNP-containing flocs	1260:1280	AgNP-containing flocs	1260:1280	Besides, the adopted AgCl-TiO2 could be recycled from AgNP-containing flocs.
29767310	4	30	dep	condition	768:776	arg1	pH = 5					779:784	pH = 5	779:784	pH = 5	779:784	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	4	30	dep	condition	768:776	arg1	TiCl4/L					805:811	dosage = 12 mg TiCl4/L	790:811	dosage = 12 mg TiCl4/L	790:811	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	2	31	theme	MSA	392:394	arg1	properties					347:356	floc properties	342:356	floc properties	342:356	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	2	31	theme	MSA	392:394	arg1	fouling					371:377	membrane fouling	362:377	membrane fouling	362:377	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	8	32	theme	anatase	1322:1328	arg1	form					1309:1312	the form	1305:1312	the form of TiO2 anatase	1305:1328	And MSA could promote the form of TiO2 anatase.
29767310	5	33	theme	subsequence	960:970	arg1	fouling					981:987	subsequence membrane fouling	960:987	subsequence membrane fouling	960:987	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	2	34	theme	TiCl4	382:386	arg1	properties					347:356	floc properties	342:356	floc properties	342:356	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	2	34	theme	TiCl4	382:386	arg1	fouling					371:377	membrane fouling	362:377	membrane fouling	362:377	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	1	35	from	water	292:296	arg1	process					329:335	coagulation-ultrafiltration process	301:335	coagulation-ultrafiltration process	301:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	4	36	dep	AgNP	715:718	arg1	removal					728:734	removal	728:734	removal	728:734	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	2	37	theme	fouling	503:509	arg1	model					511:515	ultrafiltration fouling model	487:515	ultrafiltration fouling model	487:515	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	5	38	theme	membrane	972:979	arg1	fouling					981:987	subsequence membrane fouling	960:987	subsequence membrane fouling	960:987	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	1	39	theme	modified	182:189	arg1	MSA					208:210	MSA	208:210	MSA	208:210	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	1	39	theme	modified	182:189	arg1	alginate					198:205	a modified sodium alginate	180:205	a modified sodium alginate (MSA) composited with TiCl4	180:233	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	0	40	theme	nanoparticle	22:33	arg1	coagulants					100:109	modified sodium alginate composite coagulants	65:109	modified sodium alginate composite coagulants	65:109	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	0	40	theme	nanoparticle	22:33	arg1	removal					4:10	The removal	0:10	The removal of silver nanoparticle by titanium tetrachloride	0:59	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	1	41	theme	synthetic	257:265	arg1	water					292:296	the synthetic Ag nanoparticles (AgNPs) water	253:296	the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process	253:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	0	42	theme	membrane	129:136	arg1	fouling					138:144	membrane fouling	129:144	membrane fouling	129:144	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	5	43	theme	fouling	981:987	arg1	inhibition					946:955	the inhibition	942:955	the inhibition of subsequence membrane fouling	942:987	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	2	44	theme	composite	396:404	arg1	coagulants					406:415	composite coagulants	396:415	composite coagulants (TiCl4 + MSA)	396:429	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	2	45	theme	ultrafiltration	487:501	arg1	model					511:515	ultrafiltration fouling model	487:515	ultrafiltration fouling model	487:515	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	1	46	theme	sodium	191:196	arg1	MSA					208:210	MSA	208:210	MSA	208:210	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	1	46	theme	sodium	191:196	arg1	alginate					198:205	a modified sodium alginate	180:205	a modified sodium alginate (MSA) composited with TiCl4	180:233	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	1	47	theme	Ag	267:268	arg1	water					292:296	the synthetic Ag nanoparticles (AgNPs) water	253:296	the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process	253:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	4	48	theme	optimum	760:766	arg1	condition					768:776	the optimum condition	756:776	the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L)	756:812	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	8	49	theme	TiO2	1317:1320	arg1	anatase					1322:1328	TiO2 anatase	1317:1328	TiO2 anatase	1317:1328	And MSA could promote the form of TiO2 anatase.
29767310	3	50	theme	AgNP-containing	537:551	arg1	flocs					553:557	the AgNP-containing flocs	533:557	the AgNP-containing flocs	533:557	The recycle of the AgNP-containing flocs was evaluated by XRD and photocatalytic experiments.
29767310	6	51	theme	external	1012:1019	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	51	theme	external	1012:1019	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	51	theme	external	1012:1019	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	51	theme	external	1012:1019	arg1	Ref-s					1041:1045	Ref-s	1041:1045	Ref-s	1041:1045	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	0	52	theme	titanium	38:45	arg1	tetrachloride					47:59	titanium tetrachloride	38:59	titanium tetrachloride	38:59	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	1	53	theme	nanoparticles	270:282	arg1	water					292:296	the synthetic Ag nanoparticles (AgNPs) water	253:296	the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process	253:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	2	54	dep	TiCl4	382:386	arg1	coagulants					406:415	composite coagulants	396:415	composite coagulants (TiCl4 + MSA)	396:429	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	4	55	theme	coagulation	669:679	arg1	performance					681:691	better coagulation performance	662:691	better coagulation performance	662:691	The results showed that TiCl4 + MSA could achieve better coagulation performance than TiCl4 alone with AgNP and DOC removal up to 97 and 59% at the optimum condition (pH = 5 and dosage = 12 mg TiCl4/L).
29767310	5	56	theme	looser	847:852	arg1	flocs					854:858	larger and looser flocs	836:858	larger and looser flocs	836:858	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	2	57	dep	properties	347:356	arg1	The					338:340	The	338:340	The	338:340	The floc properties and membrane fouling of TiCl4 and MSA composite coagulants (TiCl4 + MSA) were investigated by a laser diffraction instrument and ultrafiltration fouling model.
29767310	6	58	theme	coagulation	1183:1193	arg1	condition					1195:1203	the optimal coagulation condition	1171:1203	the optimal coagulation condition	1171:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	0	59	theme	floc	151:154	arg1	recycle					156:162	floc recycle	151:162	floc recycle	151:162	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	3	60	theme	photocatalytic	584:597	arg1	experiments					599:609	XRD and photocatalytic experiments	576:609	XRD and photocatalytic experiments	576:609	The recycle of the AgNP-containing flocs was evaluated by XRD and photocatalytic experiments.
29767310	6	61	theme	fouling	1021:1027	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	61	theme	fouling	1021:1027	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	61	theme	fouling	1021:1027	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	61	theme	fouling	1021:1027	arg1	Ref-s					1041:1045	Ref-s	1041:1045	Ref-s	1041:1045	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	0	62	theme	modified	65:72	arg1	coagulants					100:109	modified sodium alginate composite coagulants	65:109	modified sodium alginate composite coagulants	65:109	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	6	63	theme	attached	1003:1010	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	63	theme	attached	1003:1010	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	63	theme	attached	1003:1010	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	63	theme	attached	1003:1010	arg1	Ref-s					1041:1045	Ref-s	1041:1045	Ref-s	1041:1045	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	0	64	dep	removal	4:10	arg1	properties					117:126	floc properties	112:126	floc properties	112:126	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	0	64	dep	removal	4:10	arg1	fouling					138:144	membrane fouling	129:144	membrane fouling	129:144	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	0	64	dep	removal	4:10	arg1	recycle					156:162	floc recycle	151:162	floc recycle	151:162	The removal of silver nanoparticle by titanium tetrachloride and modified sodium alginate composite coagulants: floc properties, membrane fouling, and floc recycle.
29767310	9	65	theme	silver	1362:1367	arg1	recycle					1382:1388	silver nanoparticle recycle	1362:1388	silver nanoparticle recycle	1362:1388	It gives us a possible way for silver nanoparticle recycle.
29767310	5	66	dep	TiCl4	875:879	arg1	SA					915:916	SA	915:916	SA	915:916	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	5	66	dep	TiCl4	875:879	arg1	TiCl4					907:911	TiCl4	907:911	TiCl4	907:911	TiCl4 + MSA produced larger and looser flocs than TiCl4 and TiCl4 + SA composite coagulant (TiCl4 + SA), which was benefit for the inhibition of subsequence membrane fouling.
29767310	1	67	theme	AgNPs	285:289	arg1	water					292:296	the synthetic Ag nanoparticles (AgNPs) water	253:296	the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process	253:335	In this study, a modified sodium alginate (MSA) composited with TiCl4 was used to treat the synthetic Ag nanoparticles (AgNPs) water in coagulation-ultrafiltration process.
29767310	3	68	theme	XRD	576:578	arg1	experiments					599:609	XRD and photocatalytic experiments	576:609	XRD and photocatalytic experiments	576:609	The recycle of the AgNP-containing flocs was evaluated by XRD and photocatalytic experiments.
29767310	6	69	theme	TiCl4	1106:1110	arg1	resistance					1029:1038	The strongly attached external fouling resistance	990:1038	The strongly attached external fouling resistance (Ref-s)	990:1046	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	69	theme	TiCl4	1106:1110	arg1	Ref-s					1041:1045	Ref-s	1041:1045	Ref-s	1041:1045	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	69	theme	TiCl4	1106:1110	arg1	%					1139:1139	only 43 and 39.2%	1123:1139	only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition	1123:1203	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	69	theme	TiCl4	1106:1110	arg1	resistance					1084:1093	the reversible internal fouling resistance	1052:1093	the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA	1052:1116	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	69	theme	TiCl4	1106:1110	arg1	those					1144:1148	those	1144:1148	those	1144:1148	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
29767310	6	69	theme	TiCl4	1106:1110	arg1	Rif-r					1096:1100	Rif-r	1096:1100	Rif-r	1096:1100	The strongly attached external fouling resistance (Ref-s) and the reversible internal fouling resistance (Rif-r) of TiCl4 + MSA were only 43 and 39.2% of those achieved by TiCl4 at the optimal coagulation condition.
30889709	5	0	theme	chitosan	876:883	arg1	structure					850:858	lamellar structure	841:858	lamellar structure	841:858	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	5	0	theme	chitosan	876:883	arg1	nanoparticles					823:835	silver nanoparticles	816:835	silver nanoparticles	816:835	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	9	1	with	composites	1309:1318	arg1	%					1338:1338	hemolysis < 5%	1325:1338	hemolysis < 5%	1325:1338	Hemolysis analysis of the composites verified the hemocompatible nature of composites with hemolysis < 5%.
30889709	6	2	from	increase	943:950	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	9	3	theme	Hemolysis	1234:1242	arg1	analysis					1244:1251	Hemolysis analysis	1234:1251	Hemolysis analysis of the composites	1234:1269	Hemolysis analysis of the composites verified the hemocompatible nature of composites with hemolysis < 5%.
30889709	12	4	theme	collagen	1636:1643	arg1	fibril					1645:1650	collagen fibril	1636:1650	collagen fibril	1636:1650	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	2	5	theme	collagen	537:544	arg1	arrangement					505:515	layer-like arrangement	494:515	layer-like arrangement of native fibrillar collagen	494:544	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	6	6	theme	film	931:934	arg1	property					901:908	The mechanical property	886:908	The mechanical property of the dry composite film	886:934	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	1	7	theme	collagen	262:269	arg1	nanoparticles					238:250	silver nanoparticles	231:250	silver nanoparticles (tAgNPs)	231:259	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	7	theme	collagen	262:269	arg1	fibril					271:276	collagen fibril	262:276	collagen fibril	262:276	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	13	8	used	used	1776:1779	arg2	-could					1766:1771	the fabricated composite -could	1741:1771	the fabricated composite -could	1741:1771	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	13	8	used	used	1776:1779	arg2	biomaterial					1786:1796	a biomaterial	1784:1796	a biomaterial for bone tissue engineering applications	1784:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	13	9	theme	bone	1802:1805	arg1	applications					1826:1837	bone tissue engineering applications	1802:1837	bone tissue engineering applications	1802:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	6	10	theme	composite	921:929	arg1	film					931:934	the dry composite film	913:934	the dry composite film	913:934	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	3	11	theme	phosphates	618:627	arg1	mineralization					592:605	surface mineralization	584:605	surface mineralization of calcium phosphates incorporated with magnesium	584:655	The mineralized composites exhibited surface mineralization of calcium phosphates incorporated with magnesium.
30889709	12	12	contain	possess	1600:1606	arg2	nanoparticles					1615:1627	silver nanoparticles	1608:1627	silver nanoparticles loaded collagen fibril	1608:1650	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	12	12	contain	possess	1600:1606	arg1	composite					1584:1592	The prepared composite	1571:1592	The prepared composite	1571:1592	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	5	13	theme	lamellar	841:848	arg1	structure					850:858	lamellar structure	841:858	lamellar structure	841:858	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	13	14	theme	tissue	1807:1812	arg1	applications					1826:1837	bone tissue engineering applications	1802:1837	bone tissue engineering applications	1802:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	6	15	theme	dry	917:919	arg1	film					931:934	the dry composite film	913:934	the dry composite film	913:934	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	2	16	theme	Electron	349:356	arg1	micrograph					358:367	Electron micrograph	349:367	Electron micrograph	349:367	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	5	17	theme	structure	850:858	arg1	incorporation					799:811	incorporation	799:811	incorporation of silver nanoparticles and lamellar structure of collagen and chitosan	799:883	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	7	18	from	increase	1100:1107	arg1	mineralization					1112:1125	mineralization	1112:1125	mineralization	1112:1125	The incorporation of chitosan in M-CC3 led to 2.2 fold increase in mineralization as confirmed by the TGA analysis.
30889709	1	19	dep	nanoparticles	122:134	arg1	CC					179:180	CC	179:180	CC	179:180	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	19	dep	nanoparticles	122:134	arg1	matrix					171:176	loaded fibrillar collagen-chitosan matrix	136:176	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC)	115:181	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	10	20	theme	cell	1419:1422	arg1	%					1438:1438	cell viability > 80%	1419:1438	cell viability > 80%	1419:1438	MTT assay for the composites was carried by seeding MG-63 cells and indicated cell viability > 80%.
30889709	2	21	theme	fibrillar	428:436	arg1	morphology					438:447	native fibrillar morphology	421:447	native fibrillar morphology of collagen	421:459	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	11	22	theme	activity	1455:1462	arg1	analysis					1464:1471	Antibacterial activity analysis	1441:1471	Antibacterial activity analysis	1441:1471	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	6	23	theme	chitosan	993:1000	arg1	addition					981:988	the addition	977:988	the addition of chitosan	977:1000	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	2	24	theme	fibrillar	527:535	arg1	collagen					537:544	native fibrillar collagen	520:544	native fibrillar collagen	520:544	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	9	25	theme	composites	1309:1318	arg1	nature					1299:1304	the hemocompatible nature	1280:1304	the hemocompatible nature of composites with hemolysis < 5%	1280:1338	Hemolysis analysis of the composites verified the hemocompatible nature of composites with hemolysis < 5%.
30889709	3	26	theme	calcium	610:616	arg1	phosphates					618:627	calcium phosphates	610:627	calcium phosphates incorporated with magnesium	610:655	The mineralized composites exhibited surface mineralization of calcium phosphates incorporated with magnesium.
30889709	2	27	theme	native	421:426	arg1	morphology					438:447	native fibrillar morphology	421:447	native fibrillar morphology of collagen	421:459	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	2	28	theme	layer-like	494:503	arg1	arrangement					505:515	layer-like arrangement	494:515	layer-like arrangement of native fibrillar collagen	494:544	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	0	29	theme	fibrillar	82:90	arg1	collagen					92:99	native fibrillar collagen	75:99	native fibrillar collagen	75:99	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	8	30	theme	Contact	1161:1167	arg1	analysis					1175:1182	Contact angle analysis	1161:1182	Contact angle analysis	1161:1182	Contact angle analysis revealed the hydrophilic nature of the composite.
30889709	5	31	theme	collagen	863:870	arg1	structure					850:858	lamellar structure	841:858	lamellar structure	841:858	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	5	31	theme	collagen	863:870	arg1	nanoparticles					823:835	silver nanoparticles	816:835	silver nanoparticles	816:835	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	8	32	theme	hydrophilic	1197:1207	arg1	nature					1209:1214	the hydrophilic nature	1193:1214	the hydrophilic nature of the composite	1193:1231	Contact angle analysis revealed the hydrophilic nature of the composite.
30889709	0	33	theme	Biomimetic	0:9	arg1	fabrication					11:21	Biomimetic fabrication	0:21	Biomimetic fabrication of mineralized composite films of nanosilver	0:66	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	1	34	theme	chitosan	282:289	arg1	nanoparticles					238:250	silver nanoparticles	231:250	silver nanoparticles (tAgNPs)	231:259	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	34	theme	chitosan	282:289	arg1	hydrogel					291:298	chitosan hydrogel	282:298	chitosan hydrogel	282:298	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	6	35	theme	mechanical	890:899	arg1	property					901:908	The mechanical property	886:908	The mechanical property of the dry composite film	886:934	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	2	36	theme	composites	400:409	arg1	surface					385:391	the surface	381:391	the surface of the composites	381:409	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	0	37	theme	mineralized	26:36	arg1	films					48:52	mineralized composite films	26:52	mineralized composite films of nanosilver	26:66	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	10	38	theme	MG-63	1393:1397	arg1	cells					1399:1403	MG-63 cells	1393:1403	MG-63 cells	1393:1403	MTT assay for the composites was carried by seeding MG-63 cells and indicated cell viability > 80%.
30889709	7	39	theme	chitosan	1066:1073	arg1	incorporation					1049:1061	The incorporation	1045:1061	The incorporation of chitosan in M-CC3	1045:1082	The incorporation of chitosan in M-CC3 led to 2.2 fold increase in mineralization as confirmed by the TGA analysis.
30889709	4	40	theme	chitosan	723:730	arg1	blending					698:705	the uniform blending	686:705	the uniform blending of collagen and chitosan without any chemical interaction between them	686:776	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	4	41	theme	FT-IR	658:662	arg1	analysis					668:675	FT-IR ATR analysis	658:675	FT-IR ATR analysis	658:675	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	8	42	theme	angle	1169:1173	arg1	analysis					1175:1182	Contact angle analysis	1161:1182	Contact angle analysis	1161:1182	Contact angle analysis revealed the hydrophilic nature of the composite.
30889709	13	43	theme	fabricated	1745:1754	arg1	biomaterial					1786:1796	a biomaterial	1784:1796	a biomaterial for bone tissue engineering applications	1784:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	13	43	theme	fabricated	1745:1754	arg1	-could					1766:1771	the fabricated composite -could	1741:1771	the fabricated composite -could	1741:1771	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	1	44	theme	Silver	115:120	arg1	nanoparticles					122:134	Silver nanoparticles	115:134	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC)	115:181	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	4	45	theme	ATR	664:666	arg1	analysis					668:675	FT-IR ATR analysis	658:675	FT-IR ATR analysis	658:675	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	2	46	theme	collagen	452:459	arg1	morphology					438:447	native fibrillar morphology	421:447	native fibrillar morphology of collagen	421:459	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	0	47	theme	films	48:52	arg1	fabrication					11:21	Biomimetic fabrication	0:21	Biomimetic fabrication of mineralized composite films of nanosilver	0:66	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	3	48	theme	mineralized	551:561	arg1	composites					563:572	The mineralized composites	547:572	The mineralized composites	547:572	The mineralized composites exhibited surface mineralization of calcium phosphates incorporated with magnesium.
30889709	5	49	theme	silver	816:821	arg1	nanoparticles					823:835	silver nanoparticles	816:835	silver nanoparticles	816:835	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	1	50	theme	biomimetic	199:208	arg1	approach					210:217	biomimetic approach	199:217	biomimetic approach	199:217	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	0	51	theme	composite	38:46	arg1	films					48:52	mineralized composite films	26:52	mineralized composite films of nanosilver	26:66	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	11	52	theme	growth	1492:1497	arg1	inhibition					1499:1508	the percent growth inhibition	1480:1508	the percent growth inhibition of about 27%	1480:1521	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	2	53	theme	native	520:525	arg1	collagen					537:544	native fibrillar collagen	520:544	native fibrillar collagen	520:544	Electron micrograph showed that the surface of the composites exhibited native fibrillar morphology of collagen and their cross-section revealed layer-like arrangement of native fibrillar collagen.
30889709	11	54	theme	%	1521:1521	arg1	%					1521:1521	about 27%	1513:1521	about 27%	1513:1521	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	11	54	theme	%	1521:1521	arg1	%					1529:1529	37%	1527:1529	37% for S. aureus and E. coli	1527:1555	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	11	54	theme	%	1521:1521	arg1	inhibition					1499:1508	the percent growth inhibition	1480:1508	the percent growth inhibition of about 27%	1480:1521	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	1	55	theme	silver	231:236	arg1	tAgNPs					253:258	tAgNPs	253:258	tAgNPs	253:258	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	55	theme	silver	231:236	arg1	nanoparticles					238:250	silver nanoparticles	231:250	silver nanoparticles (tAgNPs)	231:259	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	55	theme	silver	231:236	arg1	fibril					271:276	collagen fibril	262:276	collagen fibril	262:276	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	55	theme	silver	231:236	arg1	hydrogel					291:298	chitosan hydrogel	282:298	chitosan hydrogel	282:298	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	0	56	theme	nanosilver	57:66	arg1	films					48:52	mineralized composite films	26:52	mineralized composite films of nanosilver	26:66	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	12	57	theme	formed	1680:1685	arg1	biomineral					1687:1696	the formed biomineral	1676:1696	the formed biomineral	1676:1696	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	12	57	theme	formed	1680:1685	arg1	similar					1706:1712	similar	1706:1712	similar	1706:1712	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	12	58	theme	silver	1608:1613	arg1	nanoparticles					1615:1627	silver nanoparticles	1608:1627	silver nanoparticles loaded collagen fibril	1608:1650	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	5	59	theme	nanoparticles	823:835	arg1	incorporation					799:811	incorporation	799:811	incorporation of silver nanoparticles and lamellar structure of collagen and chitosan	799:883	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	13	60	theme	composite	1756:1764	arg1	biomaterial					1786:1796	a biomaterial	1784:1796	a biomaterial for bone tissue engineering applications	1784:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	13	60	theme	composite	1756:1764	arg1	-could					1766:1771	the fabricated composite -could	1741:1771	the fabricated composite -could	1741:1771	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	5	61	theme	XRD	779:781	arg1	analysis					783:790	XRD analysis	779:790	XRD analysis	779:790	XRD analysis showed incorporation of silver nanoparticles and lamellar structure of collagen and chitosan.
30889709	6	62	theme	tensile	955:961	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	7	63	theme	fold	1095:1098	arg1	increase					1100:1107	2.2 fold increase	1091:1107	2.2 fold increase in mineralization	1091:1125	The incorporation of chitosan in M-CC3 led to 2.2 fold increase in mineralization as confirmed by the TGA analysis.
30889709	1	64	theme	loaded	136:141	arg1	CC					179:180	CC	179:180	CC	179:180	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	64	theme	loaded	136:141	arg1	matrix					171:176	loaded fibrillar collagen-chitosan matrix	136:176	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC)	115:181	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	6	65	theme	M-CC4	1028:1032	arg1	composite					1034:1042	M-CC4 composite	1028:1042	M-CC4 composite	1028:1042	The mechanical property of the dry composite film showed increase in tensile strength with the addition of chitosan and raised to 4.6 fold in M-CC4 composite.
30889709	9	66	theme	hemocompatible	1284:1297	arg1	nature					1299:1304	the hemocompatible nature	1280:1304	the hemocompatible nature of composites with hemolysis < 5%	1280:1338	Hemolysis analysis of the composites verified the hemocompatible nature of composites with hemolysis < 5%.
30889709	1	67	theme	fibrillar	143:151	arg1	CC					179:180	CC	179:180	CC	179:180	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	67	theme	fibrillar	143:151	arg1	matrix					171:176	loaded fibrillar collagen-chitosan matrix	136:176	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC)	115:181	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	12	68	theme	native	1659:1664	arg1	state					1666:1670	the native state	1655:1670	the native state	1655:1670	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	11	69	theme	Antibacterial	1441:1453	arg1	analysis					1464:1471	Antibacterial activity analysis	1441:1471	Antibacterial activity analysis	1441:1471	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	11	70	theme	percent	1484:1490	arg1	inhibition					1499:1508	the percent growth inhibition	1480:1508	the percent growth inhibition of about 27%	1480:1521	Antibacterial activity analysis showed the percent growth inhibition of about 27% and 37% for S. aureus and E. coli respectively.
30889709	1	71	theme	collagen-chitosan	153:169	arg1	CC					179:180	CC	179:180	CC	179:180	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	1	71	theme	collagen-chitosan	153:169	arg1	matrix					171:176	loaded fibrillar collagen-chitosan matrix	136:176	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC)	115:181	Silver nanoparticles loaded fibrillar collagen-chitosan matrix (CC) was prepared by biomimetic approach by blending silver nanoparticles (tAgNPs), collagen fibril and chitosan hydrogel followed by cross-linking and biomineralization.
30889709	10	72	theme	MTT	1341:1343	arg1	assay					1345:1349	MTT assay	1341:1349	MTT assay for the composites	1341:1368	MTT assay for the composites was carried by seeding MG-63 cells and indicated cell viability > 80%.
30889709	4	73	theme	uniform	690:696	arg1	blending					698:705	the uniform blending	686:705	the uniform blending of collagen and chitosan without any chemical interaction between them	686:776	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	0	74	theme	native	75:80	arg1	collagen					92:99	native fibrillar collagen	75:99	native fibrillar collagen	75:99	Biomimetic fabrication of mineralized composite films of nanosilver loaded native fibrillar collagen and chitosan.
30889709	12	75	theme	prepared	1575:1582	arg1	composite					1584:1592	The prepared composite	1571:1592	The prepared composite	1571:1592	The prepared composite would possess silver nanoparticles loaded collagen fibril in the native state and the formed biomineral will be similar to the bone mineral.
30889709	3	76	theme	surface	584:590	arg1	mineralization					592:605	surface mineralization	584:605	surface mineralization of calcium phosphates incorporated with magnesium	584:655	The mineralized composites exhibited surface mineralization of calcium phosphates incorporated with magnesium.
30889709	4	77	theme	chemical	744:751	arg1	interaction					753:763	any chemical interaction	740:763	any chemical interaction between them	740:776	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	4	78	theme	collagen	710:717	arg1	blending					698:705	the uniform blending	686:705	the uniform blending of collagen and chitosan without any chemical interaction between them	686:776	FT-IR ATR analysis revealed the uniform blending of collagen and chitosan without any chemical interaction between them.
30889709	8	79	theme	composite	1223:1231	arg1	nature					1209:1214	the hydrophilic nature	1193:1214	the hydrophilic nature of the composite	1193:1231	Contact angle analysis revealed the hydrophilic nature of the composite.
30889709	13	80	theme	engineering	1814:1824	arg1	applications					1826:1837	bone tissue engineering applications	1802:1837	bone tissue engineering applications	1802:1837	Hence the fabricated composite -could be used as a biomaterial for bone tissue engineering applications.
30889709	7	81	theme	TGA	1147:1149	arg1	analysis					1151:1158	the TGA analysis	1143:1158	the TGA analysis	1143:1158	The incorporation of chitosan in M-CC3 led to 2.2 fold increase in mineralization as confirmed by the TGA analysis.
30889709	9	82	theme	composites	1260:1269	arg1	analysis					1244:1251	Hemolysis analysis	1234:1251	Hemolysis analysis of the composites	1234:1269	Hemolysis analysis of the composites verified the hemocompatible nature of composites with hemolysis < 5%.
30889709	7	83	from	incorporation	1049:1061	arg1	M-CC3					1078:1082	M-CC3	1078:1082	M-CC3	1078:1082	The incorporation of chitosan in M-CC3 led to 2.2 fold increase in mineralization as confirmed by the TGA analysis.
31252006	4	0	with	Al2O3	716:720	arg1	ratio					729:733	a ratio	727:733	a ratio of 0, 1, 3 and 5 wt%	727:754	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	1	1	theme	aluminum	287:294	arg1	oxide					296:300	aluminum oxide	287:300	aluminum oxide nanoparticles (Al2O3)	287:322	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	8	2	theme	glass	1370:1374	arg1	temperature					1387:1397	the glass transition temperature	1366:1397	the glass transition temperature (Tg)	1366:1402	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	8	2	theme	glass	1370:1374	arg1	Tg					1400:1401	Tg	1400:1401	Tg	1400:1401	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	5	3	theme	thermal	1030:1036	arg1	stability					1038:1046	thermal stability	1030:1046	thermal stability	1030:1046	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	2	4	theme	mechanical	386:395	arg1	strength					425:432	tensile strength	417:432	tensile strength	417:432	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	2	4	theme	mechanical	386:395	arg1	properties					397:406	the mechanical properties	382:406	the mechanical properties	382:406	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	2	4	theme	mechanical	386:395	arg1	elongation					438:447	elongation	438:447	elongation	438:447	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	4	5	theme	casting	961:967	arg1	method					969:974	the solvent casting method	949:974	the solvent casting method	949:974	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	1	6	theme	oxide	296:300	arg1	Al2O3					317:321	Al2O3	317:321	Al2O3	317:321	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	6	theme	oxide	296:300	arg1	reinforcements					327:340	reinforcements	327:340	reinforcements	327:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	6	theme	oxide	296:300	arg1	nanoparticles					302:314	aluminum oxide nanoparticles	287:314	aluminum oxide nanoparticles (Al2O3)	287:322	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	6	theme	oxide	296:300	arg1	nanocrystals					264:275	cellulose nanocrystals	254:275	cellulose nanocrystals (CNC)	254:281	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	4	7	with	CNC	708:710	arg1	ratio					729:733	a ratio	727:733	a ratio of 0, 1, 3 and 5 wt%	727:754	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	2	8	from	effects	347:353	arg1	strength					425:432	tensile strength	417:432	tensile strength	417:432	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	2	8	from	effects	347:353	arg1	properties					397:406	the mechanical properties	382:406	the mechanical properties	382:406	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	2	8	from	effects	347:353	arg1	elongation					438:447	elongation	438:447	elongation	438:447	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	0	9	theme	nano	101:104	arg1	reinforcements					106:119	nano reinforcements	101:119	nano reinforcements	101:119	Preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) as green composites using nano reinforcements.
31252006	4	10	theme	two	811:813	arg1	combination					790:800	a combination	788:800	a combination of these two with the ratio of 3:3 and 5:5	788:843	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	11	11	theme	physical	1712:1719	arg1	properties					1736:1745	physical and mechanical properties	1712:1745	physical and mechanical properties of the composites	1712:1763	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	1	12	dep	preparation	148:158	arg1	the					144:146	the	144:146	the	144:146	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	9	13	theme	5 wt	1610:1613	arg1	CNC					1616:1618	5 wt% CNC	1610:1618	5 wt% CNC	1610:1618	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	9	14	theme	neat	1534:1537	arg1	film					1544:1547	neat PHBV film	1534:1547	neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively	1534:1632	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	11	15	theme	aluminum	1809:1816	arg1	nanoparticles					1824:1836	aluminum oxide nanoparticles	1809:1836	aluminum oxide nanoparticles as a nano reinforcement	1809:1860	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	11	16	theme	better	1790:1795	arg1	effect					1797:1802	a better effect	1788:1802	a better effect than aluminum oxide nanoparticles as a nano reinforcement	1788:1860	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	4	17	theme	PHBV	862:865	arg1	matrix					867:872	the PHBV matrix	858:872	the PHBV matrix	858:872	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	9	18	theme	PHBV	1539:1542	arg1	film					1544:1547	neat PHBV film	1534:1547	neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively	1534:1632	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	3	19	theme	thermal	551:557	arg1	features					559:566	thermal features	551:566	thermal features	551:566	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	7	20	from	decrease	1244:1251	arg1	composites					1285:1294	the composites	1281:1294	the composites with nanoparticles of more than 3% by weight	1281:1339	This led to a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight.
31252006	1	21	theme	PHBV	217:220	arg1	films					237:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films	184:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements	184:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	7	22	with	composites	1285:1294	arg1	nanoparticles					1301:1313	nanoparticles	1301:1313	nanoparticles of more than 3% by weight	1301:1339	This led to a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight.
31252006	5	23	theme	tensile	1009:1015	arg1	strength					1017:1024	tensile strength	1009:1024	tensile strength	1009:1024	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	6	24	theme	nanoparticles	1156:1168	arg1	nanoparticles					1156:1168	nanoparticles	1156:1168	nanoparticles (3 wt%)	1156:1176	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	6	24	theme	nanoparticles	1156:1168	arg1	%					1175:1175	3 wt%	1171:1175	3 wt%	1171:1175	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	6	24	theme	nanoparticles	1156:1168	arg1	amounts					1145:1151	large amounts	1139:1151	large amounts of nanoparticles (3 wt%)	1139:1176	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	3	25	theme	scanning	589:596	arg1	SEM					619:621	SEM	619:621	SEM	619:621	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	3	25	theme	scanning	589:596	arg1	microscopy					607:616	scanning electron microscopy	589:616	scanning electron microscopy (SEM)	589:622	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	5	26	theme	films	1064:1068	arg1	strength					1017:1024	tensile strength	1009:1024	tensile strength	1009:1024	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	5	26	theme	films	1064:1068	arg1	stability					1038:1046	thermal stability	1030:1046	thermal stability	1030:1046	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	2	27	theme	tensile	417:423	arg1	strength					425:432	tensile strength	417:432	tensile strength	417:432	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	4	28	theme	nanocomposite	912:924	arg1	films					926:930	the various nanocomposite films	900:930	the various nanocomposite films	900:930	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	10	29	theme	DMA	1688:1690	arg1	conclusions					1673:1683	the conclusions	1669:1683	the conclusions of DMA	1669:1690	These results are consistent with the conclusions of DMA.
31252006	3	30	theme	electron	598:605	arg1	SEM					619:621	SEM	619:621	SEM	619:621	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	3	30	theme	electron	598:605	arg1	microscopy					607:616	scanning electron microscopy	589:616	scanning electron microscopy (SEM)	589:622	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	1	31	theme	nanocomposite	223:235	arg1	films					237:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films	184:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements	184:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	2	32	theme	dynamic-mechanical	469:486	arg1	analysis					488:495	dynamic-mechanical analysis	469:495	dynamic-mechanical analysis (DMA)	469:501	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	2	32	theme	dynamic-mechanical	469:486	arg1	DMA					498:500	DMA	498:500	DMA	498:500	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	4	33	theme	5:5	841:843	arg1	ratio					824:828	the ratio	820:828	the ratio of 3:3 and 5:5	820:843	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	10	34	with	consistent	1653:1662	arg1	conclusions					1673:1683	the conclusions	1669:1683	the conclusions of DMA	1669:1690	These results are consistent with the conclusions of DMA.
31252006	1	35	theme	films	237:241	arg1	characterization					164:179	characterization	164:179	characterization	164:179	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	35	theme	films	237:241	arg1	preparation					148:158	preparation	148:158	preparation	148:158	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	5	36	dep	strength	1017:1024	arg1	the					1005:1007	the	1005:1007	the	1005:1007	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	1	37	theme	polyhydroxybutyrate-co-valerate	184:214	arg1	films					237:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films	184:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements	184:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	11	38	theme	composites	1754:1763	arg1	properties					1736:1745	physical and mechanical properties	1712:1745	physical and mechanical properties of the composites	1712:1763	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	11	39	theme	properties	1736:1745	arg1	improvement					1697:1707	The improvement	1693:1707	The improvement of physical and mechanical properties of the composites	1693:1763	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	7	40	theme	%	1329:1329	arg1	nanoparticles					1301:1313	nanoparticles	1301:1313	nanoparticles of more than 3% by weight	1301:1339	This led to a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight.
31252006	1	41	theme	cellulose	254:262	arg1	reinforcements					327:340	reinforcements	327:340	reinforcements	327:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	41	theme	cellulose	254:262	arg1	nanoparticles					302:314	aluminum oxide nanoparticles	287:314	aluminum oxide nanoparticles (Al2O3)	287:322	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	41	theme	cellulose	254:262	arg1	CNC					278:280	CNC	278:280	CNC	278:280	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	41	theme	cellulose	254:262	arg1	nanocrystals					264:275	cellulose nanocrystals	254:275	cellulose nanocrystals (CNC)	254:281	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	0	42	theme	polyhydroxybutyrate-co-valerate	36:66	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) as green composites using nano reinforcements.
31252006	0	42	theme	polyhydroxybutyrate-co-valerate	36:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) as green composites using nano reinforcements.
31252006	8	43	theme	participles	1454:1464	arg1	incorporation					1432:1444	the incorporation	1428:1444	the incorporation of nano participles to PHBV	1428:1472	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	8	44	theme	DSC	1342:1344	arg1	results					1346:1352	DSC results	1342:1352	DSC results	1342:1352	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	6	45	theme	large	1139:1143	arg1	nanoparticles					1156:1168	nanoparticles	1156:1168	nanoparticles (3 wt%)	1156:1176	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	6	45	theme	large	1139:1143	arg1	%					1175:1175	3 wt%	1171:1175	3 wt%	1171:1175	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	6	45	theme	large	1139:1143	arg1	amounts					1145:1151	large amounts	1139:1151	large amounts of nanoparticles (3 wt%)	1139:1176	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	11	46	theme	nano	1843:1846	arg1	reinforcement					1848:1860	a nano reinforcement	1841:1860	a nano reinforcement	1841:1860	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	4	47	dep	added	760:764	arg1	prepared					937:944	prepared	937:944	were prepared by the solvent casting method	932:974	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	4	47	dep	added	760:764	arg1	added					849:853	added	849:853	added	849:853	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	4	48	theme	various	904:910	arg1	films					926:930	the various nanocomposite films	900:930	the various nanocomposite films	900:930	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	8	49	theme	nano	1449:1452	arg1	participles					1454:1464	nano participles	1449:1464	nano participles	1449:1464	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	6	50	theme	biopolymer	1211:1220	arg1	matrix					1222:1227	the biopolymer matrix	1207:1227	the biopolymer matrix	1207:1227	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	7	51	theme	mechanical	1256:1265	arg1	properties					1267:1276	mechanical properties	1256:1276	mechanical properties	1256:1276	This led to a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight.
31252006	9	52	theme	PHBV	1578:1581	arg1	films					1583:1587	PHBV films	1578:1587	PHBV films containing 1	1578:1600	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	5	53	theme	PHBV/CNC	1055:1062	arg1	films					1064:1068	the PHBV/CNC films	1051:1068	the PHBV/CNC films	1051:1068	After adding nanoparticles, the tensile strength and thermal stability of the PHBV/CNC films increased and the elongation decreased.
31252006	2	54	dep	modulus	511:517	arg1	δ					527:527	δ	527:527	δ	527:527	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	7	55	theme	properties	1267:1276	arg1	decrease					1244:1251	a decrease	1242:1251	a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight	1242:1339	This led to a decrease of mechanical properties in the composites with nanoparticles of more than 3% by weight.
31252006	9	56	theme	%	1614:1614	arg1	CNC					1616:1618	5 wt% CNC	1610:1618	5 wt% CNC	1610:1618	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	4	57	theme	%	754:754	arg1	ratio					729:733	a ratio	727:733	a ratio of 0, 1, 3 and 5 wt%	727:754	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	4	58	theme	3:3	833:835	arg1	ratio					824:828	the ratio	820:828	the ratio of 3:3 and 5:5	820:843	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	9	59	theme	fusion	1491:1496	arg1	enthalpy					1479:1486	The enthalpy	1475:1486	The enthalpy of fusion (ΔHfus)	1475:1504	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	3	60	theme	scanning	641:648	arg1	DSC					663:665	DSC	663:665	DSC	663:665	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	3	60	theme	scanning	641:648	arg1	calorimetry					650:660	differential scanning calorimetry	628:660	differential scanning calorimetry (DSC)	628:666	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	2	61	theme	nanoparticles	365:377	arg1	effects					347:353	The effects	343:353	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation,	343:448	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	11	62	theme	oxide	1818:1822	arg1	nanoparticles					1824:1836	aluminum oxide nanoparticles	1809:1836	aluminum oxide nanoparticles as a nano reinforcement	1809:1860	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	9	63	contain	containing	1589:1598	arg2	1					1600:1600	1	1600:1600	1	1600:1600	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	9	63	contain	containing	1589:1598	arg1	films					1583:1587	PHBV films	1578:1587	PHBV films containing 1	1578:1600	The enthalpy of fusion (ΔHfus) increased from 33.8 J/g for neat PHBV film to 48.1, 50 and 45.8 J/g for PHBV films containing 1, 3, and 5 wt% CNC, respectively.
31252006	1	64	contain	containing	243:252	arg2	nanocrystals					264:275	cellulose nanocrystals	254:275	cellulose nanocrystals (CNC)	254:281	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	64	contain	containing	243:252	arg2	Al2O3					317:321	Al2O3	317:321	Al2O3	317:321	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	64	contain	containing	243:252	arg2	reinforcements					327:340	reinforcements	327:340	reinforcements	327:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	64	contain	containing	243:252	arg2	nanoparticles					302:314	aluminum oxide nanoparticles	287:314	aluminum oxide nanoparticles (Al2O3)	287:322	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	64	contain	containing	243:252	arg1	films					237:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films	184:241	polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements	184:340	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	1	64	contain	containing	243:252	arg2	CNC					278:280	CNC	278:280	CNC	278:280	This article presents the preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) nanocomposite films containing cellulose nanocrystals (CNC) and aluminum oxide nanoparticles (Al2O3) as reinforcements.
31252006	6	65	theme	SEM	1110:1112	arg1	observations					1114:1125	SEM observations	1110:1125	SEM observations	1110:1125	SEM observations showed that large amounts of nanoparticles (3 wt%) are strongly agglomerated in the biopolymer matrix.
31252006	2	66	theme	adding	358:363	arg1	nanoparticles					365:377	adding nanoparticles	358:377	adding nanoparticles	358:377	The effects of adding nanoparticles on the mechanical properties, such as tensile strength and elongation, were studied using dynamic-mechanical analysis (DMA) such as modulus and tan δ.
31252006	0	67	theme	green	78:82	arg1	composites					84:93	green composites	78:93	green composites	78:93	Preparation and characterization of polyhydroxybutyrate-co-valerate (PHBV) as green composites using nano reinforcements.
31252006	4	68	theme	solvent	953:959	arg1	method					969:974	the solvent casting method	949:974	the solvent casting method	949:974	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
31252006	11	69	theme	mechanical	1725:1734	arg1	properties					1736:1745	physical and mechanical properties	1712:1745	physical and mechanical properties of the composites	1712:1763	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	11	70	contain	has	1784:1786	arg1	CNC					1780:1782	CNC	1780:1782	CNC	1780:1782	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	11	70	contain	has	1784:1786	arg2	effect					1797:1802	a better effect	1788:1802	a better effect than aluminum oxide nanoparticles as a nano reinforcement	1788:1860	The improvement of physical and mechanical properties of the composites confirmed that CNC has a better effect than aluminum oxide nanoparticles as a nano reinforcement.
31252006	8	71	theme	transition	1376:1385	arg1	temperature					1387:1397	the glass transition temperature	1366:1397	the glass transition temperature (Tg)	1366:1402	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	8	71	theme	transition	1376:1385	arg1	Tg					1400:1401	Tg	1400:1401	Tg	1400:1401	DSC results showed that the glass transition temperature (Tg) increased slightly with the incorporation of nano participles to PHBV.
31252006	3	72	theme	differential	628:639	arg1	DSC					663:665	DSC	663:665	DSC	663:665	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	3	72	theme	differential	628:639	arg1	calorimetry					650:660	differential scanning calorimetry	628:660	differential scanning calorimetry (DSC)	628:666	Also, morphology and thermal features were investigated by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC), respectively.
31252006	4	73	with	combination	790:800	arg1	ratio					824:828	the ratio	820:828	the ratio of 3:3 and 5:5	820:843	For this purpose, first, CNC and Al2O3 with a ratio of 0, 1, 3 and 5 wt% was added to a biopolymer, then a combination of these two with the ratio of 3:3 and 5:5 was added to the PHBV matrix, separately; and finally, the various nanocomposite films were prepared by the solvent casting method.
30732745	0	0	theme	babassu	91:97	arg1	shells					107:112	babassu coconut shells	91:112	babassu coconut shells	91:112	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	3	1	theme	significant	728:738	arg1	silica					750:755	silica	750:755	silica	750:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	1	theme	significant	728:738	arg1	amount					740:745	a significant amount	726:745	a significant amount of silica	726:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	2	2	theme	babassu	357:363	arg1	endocarp					393:400	endocarp	393:400	endocarp	393:400	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	2	theme	babassu	357:363	arg1	epicarp					381:387	epicarp	381:387	epicarp	381:387	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	2	theme	babassu	357:363	arg1	shells					373:378	the babassu coconut shells	353:378	the babassu coconut shells (epicarp and endocarp)	353:401	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	4	3	theme	materials	1095:1103	arg1	production					1065:1074	the production	1061:1074	the production of cellulose-based materials of high technological interest	1061:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	2	4	theme	cellulose	464:472	arg1	acetate					474:480	cellulose acetate	464:480	cellulose acetate	464:480	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	4	5	theme	technological	1113:1125	arg1	interest					1127:1134	high technological interest	1108:1134	high technological interest	1108:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	2	6	theme	resonance	603:611	arg1	spectroscopy					613:624	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	3	7	contain	containing	770:779	arg1	composite					760:768	a composite	758:768	a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape)	758:860	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	7	contain	containing	770:779	arg2	fibers					791:796	cellulose fibers	781:796	cellulose fibers mixed with amorphous silica particles (with rosette-like shape)	781:860	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	1	8	theme	renewable	269:277	arg1	sources					286:292	renewable energy sources	269:292	renewable energy sources	269:292	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	0	9	theme	coconut	99:105	arg1	shells					107:112	babassu coconut shells	91:112	babassu coconut shells	91:112	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	3	10	theme	silica	750:755	arg1	silica					750:755	silica	750:755	silica	750:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	10	theme	silica	750:755	arg1	amount					740:745	a significant amount	726:745	a significant amount of silica	726:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	2	11	theme	magnetic	594:601	arg1	resonance					603:611	solid-state 13C nuclear magnetic resonance	570:611	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	12	theme	other	666:670	arg1	techniques					672:681	other techniques	666:681	other techniques	666:681	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	1	13	theme	energy	279:284	arg1	sources					286:292	renewable energy sources	269:292	renewable energy sources	269:292	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	2	14	dep	shells	373:378	arg1	endocarp					393:400	endocarp	393:400	endocarp	393:400	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	14	dep	shells	373:378	arg1	epicarp					381:387	epicarp	381:387	epicarp	381:387	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	14	dep	shells	373:378	arg1	shells					373:378	the babassu coconut shells	353:378	the babassu coconut shells (epicarp and endocarp)	353:401	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	3	15	theme	rosette-like	842:853	arg1	shape					855:859	rosette-like shape	842:859	rosette-like shape	842:859	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	4	16	theme	residue	1049:1055	arg1	applications					1002:1013	potential applications	992:1013	potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest	992:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	4	17	theme	lignocellulosic	1033:1047	arg1	residue					1049:1055	this important lignocellulosic residue	1018:1055	this important lignocellulosic residue for the production of cellulose-based materials of high technological interest	1018:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	1	18	theme	cellulose-	178:187	arg1	products					201:208	cellulose- and related products	178:208	cellulose- and related products	178:208	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	2	19	theme	products	436:443	arg1	achievement					421:431	the achievement	417:431	the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy	417:657	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	20	theme	electron	639:646	arg1	microscopy					648:657	scanning electron microscopy	630:657	scanning electron microscopy	630:657	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	21	theme	cellulose-silica	486:501	arg1	composite					503:511	cellulose-silica composite	486:511	cellulose-silica composite	486:511	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	4	22	theme	synthesis	909:917	arg1	possibility					894:904	the possibility	890:904	the possibility of synthesis of cellulose acetate	890:938	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	2	23	theme	scanning	630:637	arg1	microscopy					648:657	scanning electron microscopy	630:657	scanning electron microscopy	630:657	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	1	24	theme	related	193:199	arg1	products					201:208	cellulose- and related products	178:208	cellulose- and related products	178:208	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	0	25	theme	cellulose	26:34	arg1	Production					0:9	Production	0:9	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.	0:113	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	3	26	theme	cellulose	781:789	arg1	fibers					791:796	cellulose fibers	781:796	cellulose fibers mixed with amorphous silica particles (with rosette-like shape)	781:860	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	1	27	theme	products	201:208	arg1	biomass					131:137	Lignocellulosic biomass	115:137	Lignocellulosic biomass	115:137	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	1	27	theme	products	201:208	arg1	source					168:173	a source	166:173	a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials	166:336	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	0	28	theme	high-purity	14:24	arg1	cellulose					26:34	high-purity cellulose	14:34	high-purity cellulose	14:34	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	1	29	theme	vegetable	295:303	arg1	recycling					311:319	vegetable waste recycling	295:319	vegetable waste recycling	295:319	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	1	30	theme	Lignocellulosic	115:129	arg1	biomass					131:137	Lignocellulosic biomass	115:137	Lignocellulosic biomass	115:137	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	1	30	theme	Lignocellulosic	115:129	arg1	source					168:173	a source	166:173	a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials	166:336	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	4	31	theme	applications	1002:1013	arg1	plethora					980:987	the plethora	976:987	the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest	976:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	0	32	theme	cellulose	37:45	arg1	acetate					47:53	cellulose acetate	37:53	cellulose acetate	37:53	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	1	33	theme	waste	305:309	arg1	recycling					311:319	vegetable waste recycling	295:319	vegetable waste recycling	295:319	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	4	34	theme	important	1023:1031	arg1	residue					1049:1055	this important lignocellulosic residue	1018:1055	this important lignocellulosic residue for the production of cellulose-based materials of high technological interest	1018:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	4	35	theme	cellulose-based	1079:1093	arg1	materials					1095:1103	cellulose-based materials	1079:1103	cellulose-based materials of high technological interest	1079:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	3	36	theme	amorphous	809:817	arg1	particles					826:834	amorphous silica particles	809:834	amorphous silica particles (with rosette-like shape)	809:860	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	2	37	theme	nuclear	586:592	arg1	resonance					603:611	solid-state 13C nuclear magnetic resonance	570:611	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	4	38	theme	potential	992:1000	arg1	applications					1002:1013	potential applications	992:1013	potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest	992:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	4	39	theme	acetate	932:938	arg1	synthesis					909:917	synthesis	909:917	synthesis of cellulose acetate	909:938	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	0	40	theme	acetate	47:53	arg1	Production					0:9	Production	0:9	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.	0:113	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	2	41	theme	13C	582:584	arg1	resonance					603:611	solid-state 13C nuclear magnetic resonance	570:611	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	42	used	used	408:411	arg2	shells					373:378	the babassu coconut shells	353:378	the babassu coconut shells (epicarp and endocarp)	353:401	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	42	used	used	408:411	arg2	endocarp					393:400	endocarp	393:400	endocarp	393:400	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	42	used	used	408:411	arg2	epicarp					381:387	epicarp	381:387	epicarp	381:387	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	43	theme	solid-state	570:580	arg1	resonance					603:611	solid-state 13C nuclear magnetic resonance	570:611	solid-state 13C nuclear magnetic resonance spectroscopy	570:624	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	4	44	theme	interest	1127:1134	arg1	materials					1095:1103	cellulose-based materials	1079:1103	cellulose-based materials of high technological interest	1079:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	1	45	theme	great	226:230	arg1	interest					232:239	the great interest	222:239	the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials	222:336	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
30732745	4	46	theme	cellulose	922:930	arg1	acetate					932:938	cellulose acetate	922:938	cellulose acetate	922:938	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	0	47	from	shells	107:112	arg1	Production					0:9	Production	0:9	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.	0:113	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	0	48	theme	composite	76:84	arg1	Production					0:9	Production	0:9	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.	0:113	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	4	49	theme	high	1108:1111	arg1	interest					1127:1134	high technological interest	1108:1134	high technological interest	1108:1134	Finally, the possibility of synthesis of cellulose acetate was also demonstrated, illustrating the plethora of potential applications of this important lignocellulosic residue for the production of cellulose-based materials of high technological interest.
30732745	3	50	with	particles	826:834	arg1	shape					855:859	rosette-like shape	842:859	rosette-like shape	842:859	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	51	contain	contains	717:724	arg2	silica					750:755	silica	750:755	silica	750:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	51	contain	contains	717:724	arg1	precursor					692:700	this precursor	687:700	this precursor	687:700	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	51	contain	contains	717:724	arg2	amount					740:745	a significant amount	726:745	a significant amount of silica	726:755	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	3	52	theme	silica	819:824	arg1	particles					826:834	amorphous silica particles	809:834	amorphous silica particles (with rosette-like shape)	809:860	As this precursor also naturally contains a significant amount of silica, a composite containing cellulose fibers mixed with amorphous silica particles (with rosette-like shape) was also produced.
30732745	0	53	theme	cellulose-silica	59:74	arg1	composite					76:84	cellulose-silica composite	59:84	cellulose-silica composite	59:84	Production of high-purity cellulose, cellulose acetate and cellulose-silica composite from babassu coconut shells.
30732745	2	54	theme	coconut	365:371	arg1	endocarp					393:400	endocarp	393:400	endocarp	393:400	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	54	theme	coconut	365:371	arg1	epicarp					381:387	epicarp	381:387	epicarp	381:387	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	2	54	theme	coconut	365:371	arg1	shells					373:378	the babassu coconut shells	353:378	the babassu coconut shells (epicarp and endocarp)	353:401	In this work, the babassu coconut shells (epicarp and endocarp) were used for the achievement of products such as cellulose, cellulose acetate and cellulose-silica composite, which were chemically and structurally characterized by solid-state 13C nuclear magnetic resonance spectroscopy and scanning electron microscopy, among other techniques.
30732745	1	55	theme	researchers	244:254	arg1	interest					232:239	the great interest	222:239	the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials	222:336	Lignocellulosic biomass has been widely studied as a source of cellulose- and related products, attracting the great interest of researchers dealing with renewable energy sources, vegetable waste recycling and biomaterials.
31123736	16	0	theme	covariance	2219:2228	arg1	matrix					2230:2235	the covariance matrix	2215:2235	the covariance matrix of the library spectra	2215:2258	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	15	1	from	able	2098:2101	arg1	heparin					2072:2078	heparin	2072:2078	heparin	2072:2078	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	16	2	theme	library	2294:2300	arg1	spectra					2302:2308	the library spectra	2290:2308	the library spectra	2290:2308	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	15	3	theme	adulterants	2057:2067	arg1	features					2045:2052	the spectral features	2032:2052	the spectral features of adulterants	2032:2067	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	2	4	from	composition	254:264	arg1	heterogeneous					226:238	heterogeneous	226:238	heterogeneous	226:238	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	10	5	dep	analysis	1132:1139	arg1	PCA					1172:1174	PCA	1172:1174	PCA	1172:1174	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	10	5	dep	analysis	1132:1139	arg1	analysis					1162:1169	principal component analysis	1142:1169	principal component analysis (PCA)	1142:1175	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	11	6	theme	animal	1235:1240	arg1	heparins					1242:1249	animal heparins	1235:1249	animal heparins	1235:1249	This was able to distinguish animal heparins from each other, and could also separate adulterated heparin from what was considered bona fide heparin.
31123736	8	7	theme	public	920:925	arg1	emergency					934:942	a global public health emergency	911:942	a global public health emergency	911:942	Unfortunately this event was disastrous and resulted in a global public health emergency.
31123736	14	8	theme	bona	1734:1737	arg1	spectra					1744:1750	bona fide spectra	1734:1750	bona fide spectra	1734:1750	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	2	9	from	activity	281:288	arg1	heterogeneous					226:238	heterogeneous	226:238	heterogeneous	226:238	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	12	10	theme	interest	1472:1479	arg1	dataset					1461:1467	the dataset	1457:1467	the dataset of interest	1457:1479	Taught multivariate analysis functions by training the analysis to look for specific patterns within the dataset of interest.
31123736	16	11	theme	test	2319:2322	arg1	spectrum					2324:2331	the test spectrum	2315:2331	the test spectrum	2315:2331	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	8	12	theme	global	913:918	arg1	emergency					934:942	a global public health emergency	911:942	a global public health emergency	911:942	Unfortunately this event was disastrous and resulted in a global public health emergency.
31123736	15	13	theme	2D-NMR	1973:1978	arg1	spectra					1980:1986	2D-NMR spectra	1973:1986	2D-NMR spectra	1973:1986	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	13	14	theme	possible	1604:1611	arg1	signal					1619:1624	every possible alien signal	1598:1624	every possible alien signal	1598:1624	If this approach was to be applied to heparin, or any other biological medicine, it would have to be taught to find every possible alien signal.
31123736	3	15	dep	example	355:361	arg1	insulin					363:369	insulin	363:369	insulin	363:369	Some of the oldest pharmaceutical products are biologicals, for example insulin and heparin.
31123736	15	16	from	heparin	2072:2078	arg1	able					2098:2101	able	2098:2101	able	2098:2101	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	1	17	theme	biological	65:74	arg1	medicine					76:83	A biological medicine	63:83	A biological medicine (or biologicals)	63:100	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	1	17	theme	biological	65:74	arg1	biologicals					89:99	biologicals	89:99	biologicals	89:99	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	1	17	theme	biological	65:74	arg1	term					107:110	a term	105:110	a term for a medicinal compound that is derived from a living organism	105:174	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	6	18	theme	animals	651:657	arg1	mucosa					641:646	the intestinal mucosa	626:646	the intestinal mucosa of animals	626:657	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	14	19	theme	opposite	1631:1638	arg1	efficient					1663:1671	efficient	1663:1671	efficient	1663:1671	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	14	19	theme	opposite	1631:1638	arg1	approach					1640:1647	The opposite approach	1627:1647	The opposite approach	1627:1647	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	3	20	theme	oldest	303:308	arg1	products					325:332	the oldest pharmaceutical products	299:332	the oldest pharmaceutical products	299:332	Some of the oldest pharmaceutical products are biologicals, for example insulin and heparin.
31123736	9	21	theme	NMR	1019:1021	arg1	spectroscopy					1023:1034	NMR spectroscopy	1019:1034	NMR spectroscopy	1019:1034	This was the impetuous to apply modern analytical techniques, principally NMR spectroscopy, and multivariate analyses to monitor heparin.
31123736	6	22	theme	non-porcine	727:737	arg1	heparin					739:745	non-porcine heparin	727:745	non-porcine heparin due to social and economical reasons	727:782	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	12	23	theme	Taught	1356:1361	arg1	analysis					1376:1383	Taught multivariate analysis	1356:1383	Taught multivariate analysis	1356:1383	Taught multivariate analysis functions by training the analysis to look for specific patterns within the dataset of interest.
31123736	7	24	theme	sulfated	830:837	arg1	polysaccharide					839:852	another sulfated polysaccharide	822:852	another sulfated polysaccharide	822:852	In 2008 heparin was adulterated with another sulfated polysaccharide.
31123736	17	25	theme	vaccine	2418:2424	arg1	polysaccharides					2426:2440	vaccine polysaccharides	2418:2440	vaccine polysaccharides	2418:2440	These approaches are universal and could be applied to biological medicines such as vaccine polysaccharides and monoclonal antibodies.
31123736	3	26	theme	pharmaceutical	310:323	arg1	products					325:332	the oldest pharmaceutical products	299:332	the oldest pharmaceutical products	299:332	Some of the oldest pharmaceutical products are biologicals, for example insulin and heparin.
31123736	17	27	theme	biological	2389:2398	arg1	polysaccharides					2426:2440	vaccine polysaccharides	2418:2440	vaccine polysaccharides	2418:2440	These approaches are universal and could be applied to biological medicines such as vaccine polysaccharides and monoclonal antibodies.
31123736	17	27	theme	biological	2389:2398	arg1	antibodies					2457:2466	monoclonal antibodies	2446:2466	monoclonal antibodies	2446:2466	These approaches are universal and could be applied to biological medicines such as vaccine polysaccharides and monoclonal antibodies.
31123736	17	27	theme	biological	2389:2398	arg1	medicines					2400:2408	biological medicines	2389:2408	biological medicines such as vaccine polysaccharides and monoclonal antibodies	2389:2466	These approaches are universal and could be applied to biological medicines such as vaccine polysaccharides and monoclonal antibodies.
31123736	13	28	theme	biological	1542:1551	arg1	medicine					1553:1560	any other biological medicine	1532:1560	any other biological medicine	1532:1560	If this approach was to be applied to heparin, or any other biological medicine, it would have to be taught to find every possible alien signal.
31123736	15	29	theme	spectral	2036:2043	arg1	features					2045:2052	the spectral features	2032:2052	the spectral features of adulterants	2032:2067	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	10	30	theme	unsupervised	1106:1117	arg1	analysis					1132:1139	traditional unsupervised multivariate analysis	1094:1139	traditional unsupervised multivariate analysis (principal component analysis (PCA))	1094:1176	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	14	31	with	consistent	1841:1850	arg1	library					1871:1877	the reference library	1857:1877	the reference library	1857:1877	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	17	32	theme	monoclonal	2446:2455	arg1	antibodies					2457:2466	monoclonal antibodies	2446:2466	monoclonal antibodies	2446:2466	These approaches are universal and could be applied to biological medicines such as vaccine polysaccharides and monoclonal antibodies.
31123736	6	33	theme	intestinal	630:639	arg1	mucosa					641:646	the intestinal mucosa	626:646	the intestinal mucosa of animals	626:657	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	14	34	theme	alien	1813:1817	arg1	features					1819:1826	alien features	1813:1826	alien features that are not consistent with the reference library	1813:1877	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	0	35	theme	Multivariate	0:11	arg1	analysis					13:20	Multivariate analysis	0:20	Multivariate analysis	0:20	Multivariate analysis applied to complex biological medicines.
31123736	12	36	theme	specific	1432:1439	arg1	patterns					1441:1448	specific patterns	1432:1448	specific patterns within the dataset of interest	1432:1479	Taught multivariate analysis functions by training the analysis to look for specific patterns within the dataset of interest.
31123736	6	37	theme	significant	704:714	arg1	market					716:721	a significant market	702:721	a significant market for non-porcine heparin due to social and economical reasons	702:782	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	2	38	theme	biological	270:279	arg1	activity					281:288	biological activity	270:288	biological activity	270:288	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	9	39	theme	multivariate	1041:1052	arg1	analyses					1054:1061	multivariate analyses	1041:1061	multivariate analyses	1041:1061	This was the impetuous to apply modern analytical techniques, principally NMR spectroscopy, and multivariate analyses to monitor heparin.
31123736	1	40	theme	living	160:165	arg1	organism					167:174	a living organism	158:174	a living organism	158:174	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	4	41	theme	chemical	498:505	arg1	entity					507:512	a defined chemical entity	488:512	a defined chemical entity	488:512	The former is now produced recombinantly, with technology being at a point where this can be considered a defined chemical entity.
31123736	15	42	theme	approach	1904:1911	arg1	This					1880:1883	This	1880:1883	This	1880:1883	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	15	42	theme	approach	1904:1911	arg1	basis					1892:1896	the basis	1888:1896	the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products	1888:2149	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	8	43	theme	health	927:932	arg1	emergency					934:942	a global public health emergency	911:942	a global public health emergency	911:942	Unfortunately this event was disastrous and resulted in a global public health emergency.
31123736	15	44	theme	spectral	1920:1927	arg1	filtering					1929:1937	spectral filtering	1920:1937	spectral filtering	1920:1937	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	16	45	theme	spectra	2252:2258	arg1	matrix					2230:2235	the covariance matrix	2215:2235	the covariance matrix of the library spectra	2215:2258	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	12	46	theme	multivariate	1363:1374	arg1	analysis					1376:1383	Taught multivariate analysis	1356:1383	Taught multivariate analysis	1356:1383	Taught multivariate analysis functions by training the analysis to look for specific patterns within the dataset of interest.
31123736	15	47	theme	biosimilar	2131:2140	arg1	products					2142:2149	supposedly biosimilar products	2120:2149	supposedly biosimilar products	2120:2149	This is the basis of an approach termed spectral filtering, which has been applied to 1D and 2D-NMR spectra, and has been very successful in extracting the spectral features of adulterants in heparin, as well as being able to differentiate supposedly biosimilar products.
31123736	13	48	theme	other	1536:1540	arg1	medicine					1553:1560	any other biological medicine	1532:1560	any other biological medicine	1532:1560	If this approach was to be applied to heparin, or any other biological medicine, it would have to be taught to find every possible alien signal.
31123736	0	49	theme	biological	41:50	arg1	medicines					52:60	complex biological medicines	33:60	complex biological medicines	33:60	Multivariate analysis applied to complex biological medicines.
31123736	14	50	dep	bona	1734:1737	arg1	fide					1739:1742	fide	1739:1742	fide	1739:1742	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	1	51	attach	derived	145:151	arg2	compound					128:135	a medicinal compound	116:135	a medicinal compound that is derived from a living organism	116:174	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	1	51	attach	derived	145:151	arg1	organism					167:174	a living organism	158:174	a living organism	158:174	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	9	52	theme	modern	977:982	arg1	techniques					995:1004	modern analytical techniques	977:1004	modern analytical techniques	977:1004	This was the impetuous to apply modern analytical techniques, principally NMR spectroscopy, and multivariate analyses to monitor heparin.
31123736	16	53	theme	library	2244:2250	arg1	spectra					2252:2258	the library spectra	2240:2258	the library spectra	2240:2258	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	0	54	theme	complex	33:39	arg1	medicines					52:60	complex biological medicines	33:60	complex biological medicines	33:60	Multivariate analysis applied to complex biological medicines.
31123736	11	55	dep	bona	1337:1340	arg1	fide					1342:1345	fide	1342:1345	fide	1342:1345	This was able to distinguish animal heparins from each other, and could also separate adulterated heparin from what was considered bona fide heparin.
31123736	14	56	with	samples	1776:1782	arg1	spectra					1795:1801	these spectra	1789:1801	these spectra	1789:1801	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	10	57	theme	multivariate	1119:1130	arg1	analysis					1132:1139	traditional unsupervised multivariate analysis	1094:1139	traditional unsupervised multivariate analysis (principal component analysis (PCA))	1094:1176	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	14	58	theme	reference	1861:1869	arg1	library					1871:1877	the reference library	1857:1877	the reference library	1857:1877	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	11	59	theme	adulterated	1292:1302	arg1	heparin					1304:1310	adulterated heparin	1292:1310	adulterated heparin	1292:1310	This was able to distinguish animal heparins from each other, and could also separate adulterated heparin from what was considered bona fide heparin.
31123736	4	60	theme	defined	490:496	arg1	entity					507:512	a defined chemical entity	488:512	a defined chemical entity	488:512	The former is now produced recombinantly, with technology being at a point where this can be considered a defined chemical entity.
31123736	2	61	theme	very	186:189	arg1	nature					191:196	their very nature	180:196	their very nature	180:196	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	14	62	theme	test	1771:1774	arg1	samples					1776:1782	test samples	1771:1782	test samples with these spectra	1771:1801	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	10	63	theme	traditional	1094:1104	arg1	analysis					1132:1139	traditional unsupervised multivariate analysis	1094:1139	traditional unsupervised multivariate analysis (principal component analysis (PCA))	1094:1176	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	14	64	theme	complex	1687:1693	arg1	material					1709:1716	the complex heterogeneous material	1683:1716	the complex heterogeneous material	1683:1716	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	6	65	theme	economical	765:774	arg1	reasons					776:782	social and economical reasons	754:782	social and economical reasons	754:782	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	13	66	dep	possible	1604:1611	arg1	alien					1613:1617	alien	1613:1617	alien	1613:1617	If this approach was to be applied to heparin, or any other biological medicine, it would have to be taught to find every possible alien signal.
31123736	16	67	theme	covariance	2269:2278	arg1	matrix					2280:2285	the covariance matrix	2265:2285	the covariance matrix of the library spectra plus the test spectrum	2265:2331	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	2	68	from	heterogeneous	226:238	arg1	structure					243:251	structure	243:251	structure	243:251	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	2	68	from	heterogeneous	226:238	arg1	composition					254:264	composition	254:264	composition	254:264	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	2	68	from	heterogeneous	226:238	arg1	activity					281:288	biological activity	270:288	biological activity	270:288	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	16	69	theme	filtered	2168:2175	arg1	spectrum					2177:2184	the filtered spectrum	2164:2184	the filtered spectrum	2164:2184	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	16	70	theme	spectrum	2324:2331	arg1	matrix					2280:2285	the covariance matrix	2265:2285	the covariance matrix of the library spectra plus the test spectrum	2265:2331	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	6	71	theme	heterogeneous	574:586	arg1	Heparin					561:567	Heparin	561:567	Heparin	561:567	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	6	71	theme	heterogeneous	574:586	arg1	polysaccharide					588:601	a heterogeneous polysaccharide	572:601	a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons	572:782	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	10	72	theme	component	1152:1160	arg1	PCA					1172:1174	PCA	1172:1174	PCA	1172:1174	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	10	72	theme	component	1152:1160	arg1	analysis					1162:1169	principal component analysis	1142:1169	principal component analysis (PCA)	1142:1175	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	6	73	theme	social	754:759	arg1	reasons					776:782	social and economical reasons	754:782	social and economical reasons	754:782	Heparin is a heterogeneous polysaccharide that is extracted from the intestinal mucosa of animals, primarily porcine, although there is also a significant market for non-porcine heparin due to social and economical reasons.
31123736	1	74	theme	medicinal	118:126	arg1	compound					128:135	a medicinal compound	116:135	a medicinal compound that is derived from a living organism	116:174	A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism.
31123736	2	75	from	structure	243:251	arg1	heterogeneous					226:238	heterogeneous	226:238	heterogeneous	226:238	By their very nature, they are complex and often heterogeneous in structure, composition and biological activity.
31123736	10	76	theme	principal	1142:1150	arg1	PCA					1172:1174	PCA	1172:1174	PCA	1172:1174	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	10	76	theme	principal	1142:1150	arg1	analysis					1162:1169	principal component analysis	1142:1169	principal component analysis (PCA)	1142:1175	Initially, traditional unsupervised multivariate analysis (principal component analysis (PCA)) was applied to the problem.
31123736	11	77	theme	bona	1337:1340	arg1	heparin					1347:1353	bona fide heparin	1337:1353	bona fide heparin	1337:1353	This was able to distinguish animal heparins from each other, and could also separate adulterated heparin from what was considered bona fide heparin.
31123736	16	78	theme	spectra	2302:2308	arg1	matrix					2280:2285	the covariance matrix	2265:2285	the covariance matrix of the library spectra plus the test spectrum	2265:2331	In essence, the filtered spectrum is determined by subtracting the covariance matrix of the library spectra from the covariance matrix of the library spectra plus the test spectrum.
31123736	14	79	theme	spectra	1744:1750	arg1	library					1723:1729	a library	1721:1729	a library of bona fide spectra	1721:1750	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
31123736	9	80	theme	analytical	984:993	arg1	techniques					995:1004	modern analytical techniques	977:1004	modern analytical techniques	977:1004	This was the impetuous to apply modern analytical techniques, principally NMR spectroscopy, and multivariate analyses to monitor heparin.
31123736	14	81	theme	heterogeneous	1695:1707	arg1	material					1709:1716	the complex heterogeneous material	1683:1716	the complex heterogeneous material	1683:1716	The opposite approach would be more efficient; defining the complex heterogeneous material by a library of bona fide spectra and then filtering test samples with these spectra to reveal alien features that are not consistent with the reference library.
29426424	0	0	theme	high-amylose	69:80	arg1	potato					82:87	a high-amylose potato	67:87	a high-amylose potato	67:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	4	1	theme	amylose	648:654	arg1	content					656:662	amylose content	648:662	amylose content	648:662	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	2	2	theme	high-amylose	312:323	arg1	tubers					325:330	the high-amylose tubers	308:330	the high-amylose tubers (30% of dry matter, DM)	308:354	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	3	theme	dry	340:342	arg1	matter					344:349	dry matter	340:349	dry matter	340:349	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	4	contain	had	356:358	arg2	RS					371:372	much lower RS	360:372	much lower RS	360:372	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	4	contain	had	356:358	arg1	tubers					325:330	the high-amylose tubers	308:330	the high-amylose tubers (30% of dry matter, DM)	308:354	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	3	5	dep	gave	458:461	arg1	%					501:501	4%	500:501	4% of DM	500:507	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	5	dep	gave	458:461	arg1	DM					479:480	DM	479:480	DM	479:480	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	5	dep	gave	458:461	arg1	%					474:474	13%	472:474	13% of DM	472:480	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	5	dep	gave	458:461	arg1	DM					506:507	DM	506:507	DM	506:507	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	1	6	theme	dietary	234:240	arg1	DF					249:250	DF	249:250	DF	249:250	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	1	6	theme	dietary	234:240	arg1	fiber					242:246	dietary fiber	234:246	dietary fiber (DF)	234:251	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	2	7	dep	tubers	390:395	arg1	%					400:400	56%	398:400	56% of DM	398:406	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	7	dep	tubers	390:395	arg1	DM					405:406	DM	405:406	DM	405:406	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	6	8	theme	less	960:963	arg1	pectin					965:970	more cellulose and less pectin	941:970	more cellulose and less pectin	941:970	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	6	9	theme	cellulose	946:954	arg1	pectin					965:970	more cellulose and less pectin	941:970	more cellulose and less pectin	941:970	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	4	10	from	changes	637:643	arg1	structure					680:688	starch granule structure	665:688	starch granule structure	665:688	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	4	10	from	changes	637:643	arg1	content					656:662	amylose content	648:662	amylose content	648:662	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	4	10	from	changes	637:643	arg1	structure					707:715	amylopectin structure	695:715	amylopectin structure	695:715	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	1	11	from	line	138:141	arg1	Tubers					90:95	Tubers	90:95	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo	90:188	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	6	12	theme	genetic	912:918	arg1	modification					920:931	the genetic modification	908:931	the genetic modification	908:931	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	5	13	dep	more	793:796	arg1	DF					798:799	DF	798:799	DF (10-14% of DM)	798:814	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	0	14	from	components	41:50	arg1	tubers					55:60	tubers	55:60	tubers from a high-amylose potato	55:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	0	14	from	components	41:50	arg1	potato					82:87	a high-amylose potato	67:87	a high-amylose potato	67:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	5	15	theme	high-amylose	758:769	arg1	tubers					771:776	The high-amylose tubers	754:776	The high-amylose tubers	754:776	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	1	16	theme	parental	158:165	arg1	Dinamo					183:188	its parental potato cultivar Dinamo	154:188	its parental potato cultivar Dinamo	154:188	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	2	17	theme	DM	405:406	arg1	%					400:400	56%	398:400	56% of DM	398:406	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	17	theme	DM	405:406	arg1	DM					405:406	DM	405:406	DM	405:406	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	3	18	theme	RS	519:520	arg1	level					522:526	the RS level	515:526	the RS level	515:526	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	0	19	theme	Resistant	0:8	arg1	starch					10:15	Resistant starch	0:15	Resistant starch	0:15	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	1	20	theme	potato	167:172	arg1	Dinamo					183:188	its parental potato cultivar Dinamo	154:188	its parental potato cultivar Dinamo	154:188	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	6	21	theme	high-amylose	979:990	arg1	tubers					992:997	the high-amylose tubers	975:997	the high-amylose tubers than the parent	975:1013	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	5	22	theme	DM	841:842	arg1	DM					841:842	DM	841:842	DM	841:842	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	5	22	theme	DM	841:842	arg1	%					836:836	5-7%	833:836	5-7% of DM	833:842	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	1	23	theme	cultivar	174:181	arg1	Dinamo					183:188	its parental potato cultivar Dinamo	154:188	its parental potato cultivar Dinamo	154:188	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	2	24	theme	lower	365:369	arg1	RS					371:372	much lower RS	360:372	much lower RS	360:372	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	4	25	theme	amylopectin	695:705	arg1	structure					707:715	amylopectin structure	695:715	amylopectin structure	695:715	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	2	26	theme	matter	344:349	arg1	DM					352:353	DM	352:353	DM	352:353	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	26	theme	matter	344:349	arg1	%					335:335	30%	333:335	30% of dry matter	333:349	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	26	theme	matter	344:349	arg1	matter					344:349	dry matter	340:349	dry matter	340:349	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	3	27	theme	high-amylose	438:449	arg1	tubers					451:456	the high-amylose tubers	434:456	the high-amylose tubers	434:456	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	5	28	dep	DF	798:799	arg1	DM					812:813	DM	812:813	DM	812:813	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	5	28	dep	DF	798:799	arg1	%					807:807	10-14%	802:807	10-14% of DM	802:813	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	0	29	theme	fiber	35:39	arg1	components					41:50	fiber components	35:50	fiber components in tubers from a high-amylose potato	35:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	4	30	theme	altered	598:604	arg1	attributable					621:632	attributable	621:632	attributable	621:632	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	4	30	theme	altered	598:604	arg1	content					609:615	The altered RS content	594:615	The altered RS content	594:615	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	2	31	theme	parent	383:388	arg1	tubers					390:395	the parent tubers	379:395	the parent tubers (56% of DM)	379:407	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	5	32	dep	contained	783:791	arg1	DM					841:842	DM	841:842	DM	841:842	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	5	32	dep	contained	783:791	arg1	%					836:836	5-7%	833:836	5-7% of DM	833:842	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	1	33	theme	resistant	208:216	arg1	RS					226:227	RS	226:227	RS	226:227	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	1	33	theme	resistant	208:216	arg1	starch					218:223	resistant starch	208:223	resistant starch (RS)	208:228	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	1	34	theme	cold	271:274	arg1	storage					276:282	cold storage	271:282	cold storage	271:282	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	4	35	theme	granule	672:678	arg1	structure					680:688	starch granule structure	665:688	starch granule structure	665:688	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	3	36	theme	more	463:466	arg1	RS					468:469	more RS	463:469	more RS	463:469	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	6	37	theme	wall	864:867	arg1	composition					869:879	cell wall composition	859:879	cell wall composition	859:879	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	4	38	theme	starch	665:670	arg1	structure					680:688	starch granule structure	665:688	starch granule structure	665:688	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	5	39	theme	DM	812:813	arg1	DM					812:813	DM	812:813	DM	812:813	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	5	39	theme	DM	812:813	arg1	%					807:807	10-14%	802:807	10-14% of DM	802:813	The high-amylose tubers also contained more DF (10-14% of DM) than the parent (5-7% of DM).
29426424	4	40	theme	RS	606:607	arg1	attributable					621:632	attributable	621:632	attributable	621:632	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	4	40	theme	RS	606:607	arg1	content					609:615	The altered RS content	594:615	The altered RS content	594:615	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	6	41	theme	cell	859:862	arg1	composition					869:879	cell wall composition	859:879	cell wall composition	859:879	Furthermore, cell wall composition was indirectly affected by the genetic modification, giving more cellulose and less pectin in the high-amylose tubers than the parent.
29426424	3	42	theme	DM	562:563	arg1	DM					562:563	DM	562:563	DM	562:563	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	42	theme	DM	562:563	arg1	%					557:557	about 20%	549:557	about 20% of DM	549:563	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	43	theme	DM	506:507	arg1	%					501:501	4%	500:501	4% of DM	500:507	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	43	theme	DM	506:507	arg1	DM					506:507	DM	506:507	DM	506:507	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	44	theme	DM	479:480	arg1	DM					479:480	DM	479:480	DM	479:480	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	3	44	theme	DM	479:480	arg1	%					474:474	13%	472:474	13% of DM	472:480	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	1	45	theme	modified	116:123	arg1	line					138:141	a genetically modified high-amylose line T-2012	102:148	a genetically modified high-amylose line T-2012	102:148	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	3	46	theme	storage	585:591	arg1	1 day					571:575	1 day	571:575	1 day of cold storage	571:591	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
29426424	2	47	dep	tubers	325:330	arg1	DM					352:353	DM	352:353	DM	352:353	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	47	dep	tubers	325:330	arg1	%					335:335	30%	333:335	30% of dry matter	333:349	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	2	47	dep	tubers	325:330	arg1	matter					344:349	dry matter	340:349	dry matter	340:349	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	1	48	theme	high-amylose	125:136	arg1	line					138:141	a genetically modified high-amylose line T-2012	102:148	a genetically modified high-amylose line T-2012	102:148	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	2	49	theme	uncooked	289:296	arg1	potatoes					298:305	uncooked potatoes	289:305	uncooked potatoes	289:305	For uncooked potatoes, the high-amylose tubers (30% of dry matter, DM) had much lower RS than the parent tubers (56% of DM).
29426424	0	50	from	potato	82:87	arg1	tubers					55:60	tubers	55:60	tubers from a high-amylose potato	55:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	0	50	from	potato	82:87	arg1	components					41:50	fiber components	35:50	fiber components in tubers from a high-amylose potato	35:87	Resistant starch and other dietary fiber components in tubers from a high-amylose potato.
29426424	1	51	from	Dinamo	183:188	arg1	Tubers					90:95	Tubers	90:95	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo	90:188	Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) and dietary fiber (DF) after cooking and cold storage.
29426424	4	52	theme	genetic	732:738	arg1	modification					740:751	the genetic modification	728:751	the genetic modification	728:751	The altered RS content was attributable to changes in amylose content, starch granule structure, and amylopectin structure induced by the genetic modification.
29426424	3	53	theme	cold	580:583	arg1	storage					585:591	cold storage	580:591	cold storage	580:591	However, after cooking, the high-amylose tubers gave more RS (13% of DM) than the parent (4% of DM), and the RS level increased further to about 20% of DM after 1 day of cold storage.
31253275	0	0	theme	pigmented	74:82	arg1	starches					89:96	pigmented rice starches	74:96	pigmented rice starches	74:96	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	2	1	theme	thermal	502:508	arg1	characteristics					510:524	physicochemical, pasting and thermal characteristics	473:524	physicochemical, pasting and thermal characteristics	473:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	5	2	dep	quder	828:832	arg1	cultivars					854:862	kareed rice cultivars	842:862	kareed rice cultivars	842:862	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	2	3	from	hybrids	426:432	arg1	terms					464:468	terms	464:468	terms of physicochemical, pasting and thermal characteristics	464:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	4	4	theme	starch	719:724	arg1	samples					726:732	the analyzed starch samples	706:732	the analyzed starch samples	706:732	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	1	5	theme	crystalline	242:252	arg1	structure					254:262	crystalline structure	242:262	crystalline structure	242:262	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	5	6	theme	compact	787:793	arg1	nature					795:800	The compact nature	783:800	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars	783:862	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	5	7	from	nature	795:800	arg1	Kaw					838:840	Kaw	838:840	Kaw	838:840	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	5	7	from	nature	795:800	arg1	quder					828:832	quder	828:832	quder	828:832	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	2	8	theme	cultivars	355:363	arg1	starches					332:339	The starches	328:339	The starches of these rice cultivars	328:363	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	1	9	theme	structure	254:262	arg1	effect					187:192	the effect	183:192	the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics	183:325	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	0	10	theme	starches	89:96	arg1	composition					19:29	chemical composition	10:29	chemical composition	10:29	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	0	10	theme	starches	89:96	arg1	structure					40:48	granule structure	32:48	granule structure	32:48	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	0	10	theme	starches	89:96	arg1	form					66:69	crystalline form	54:69	crystalline form	54:69	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	0	11	from	Effect	0:5	arg1	characteristics					118:132	their functional characteristics	101:132	their functional characteristics	101:132	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	7	12	theme	shaped	1155:1160	arg1	morphology					1162:1171	a polyhedral angular shaped morphology	1134:1171	a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1134:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	3	13	theme	particles	563:571	arg1	diameter					531:538	The diameter	527:538	The diameter of the analyzed starch particles	527:571	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	4	14	theme	whiteness	761:769	arg1	degree					751:756	a high degree	744:756	a high degree of whiteness and purity	744:780	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	0	15	theme	rice	84:87	arg1	starches					89:96	pigmented rice starches	74:96	pigmented rice starches	74:96	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	4	16	theme	purity	775:780	arg1	degree					751:756	a high degree	744:756	a high degree of whiteness and purity	744:780	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	1	17	theme	pigmented	267:275	arg1	starches					282:289	pigmented rice starches	267:289	pigmented rice starches	267:289	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	4	18	theme	analyzed	710:717	arg1	samples					726:732	the analyzed starch samples	706:732	the analyzed starch samples	706:732	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	7	19	theme	polyhedral	1136:1145	arg1	morphology					1162:1171	a polyhedral angular shaped morphology	1134:1171	a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1134:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	5	20	theme	kareed	842:847	arg1	cultivars					854:862	kareed rice cultivars	842:862	kareed rice cultivars	842:862	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	3	21	theme	analyzed	547:554	arg1	particles					563:571	the analyzed starch particles	543:571	the analyzed starch particles	543:571	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	5	22	theme	granules	812:819	arg1	nature					795:800	The compact nature	783:800	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars	783:862	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	2	23	theme	pasting	490:496	arg1	characteristics					510:524	physicochemical, pasting and thermal characteristics	473:524	physicochemical, pasting and thermal characteristics	473:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	3	24	theme	starch	556:561	arg1	particles					563:571	the analyzed starch particles	543:571	the analyzed starch particles	543:571	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	6	25	theme	A-type	1001:1006	arg1	pattern					1008:1014	an A-type pattern	998:1014	an A-type pattern for analyzing starches	998:1037	Crystalline pattern by X-Ray diffractometer showed an A-type pattern for analyzing starches.
31253275	2	26	theme	novel	390:394	arg1	characteristic					396:409	novel characteristic	390:409	novel characteristic	390:409	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	1	27	theme	rice	277:280	arg1	starches					282:289	pigmented rice starches	267:289	pigmented rice starches	267:289	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	4	28	theme	color	690:694	arg1	values					696:701	The color values	686:701	The color values of the analyzed starch samples	686:732	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	0	29	theme	composition	19:29	arg1	Effect					0:5	Effect	0:5	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.	0:133	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	1	30	theme	starches	282:289	arg1	morphology					227:236	granule morphology	219:236	granule morphology	219:236	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	1	30	theme	starches	282:289	arg1	structure					254:262	crystalline structure	242:262	crystalline structure	242:262	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	1	30	theme	starches	282:289	arg1	composition					206:216	chemical composition	197:216	chemical composition	197:216	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	7	31	theme	spherical	1108:1116	arg1	granules					1118:1125	spherical granules	1108:1125	spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1108:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	5	32	theme	starch	805:810	arg1	granules					812:819	starch granules	805:819	starch granules	805:819	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	0	33	theme	chemical	10:17	arg1	composition					19:29	chemical composition	10:29	chemical composition	10:29	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	0	34	theme	functional	107:116	arg1	characteristics					118:132	their functional characteristics	101:132	their functional characteristics	101:132	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	2	35	contain	possess	382:388	arg1	starches					332:339	The starches	328:339	The starches of these rice cultivars	328:363	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	2	35	contain	possess	382:388	arg2	characteristic					396:409	novel characteristic	390:409	novel characteristic	390:409	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	3	36	theme	considerable	584:595	arg1	range					597:601	a considerable range	582:601	a considerable range varying from 5.139 μm	582:623	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	5	37	theme	transition	891:900	arg1	temperatures					902:913	higher transition temperatures	884:913	their higher transition temperatures	878:913	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	0	38	theme	granule	32:38	arg1	structure					40:48	granule structure	32:48	granule structure	32:48	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	5	39	theme	other	930:934	arg1	cultivars					936:944	other cultivars	930:944	other cultivars	930:944	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	5	40	theme	rice	849:852	arg1	cultivars					854:862	kareed rice cultivars	842:862	kareed rice cultivars	842:862	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	5	41	theme	higher	884:889	arg1	temperatures					902:913	higher transition temperatures	884:913	their higher transition temperatures	878:913	The compact nature of starch granules in Kaw quder and Kaw kareed rice cultivars accounted for their higher transition temperatures as compared to other cultivars.
31253275	1	42	theme	chemical	197:204	arg1	composition					206:216	chemical composition	197:216	chemical composition	197:216	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	7	43	theme	smooth	1206:1211	arg1	surface					1213:1219	a relatively smooth surface	1193:1219	a relatively smooth surface	1193:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	1	44	theme	functional	300:309	arg1	characteristics					311:325	their functional characteristics	294:325	their functional characteristics	294:325	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	7	45	theme	starch	1044:1049	arg1	granules					1051:1058	The starch granules	1040:1058	The starch granules	1040:1058	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	1	46	theme	composition	206:216	arg1	effect					187:192	the effect	183:192	the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics	183:325	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	2	47	theme	physicochemical	473:487	arg1	characteristics					510:524	physicochemical, pasting and thermal characteristics	473:524	physicochemical, pasting and thermal characteristics	473:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	6	48	theme	X-Ray	970:974	arg1	diffractometer					976:989	X-Ray diffractometer	970:989	X-Ray diffractometer	970:989	Crystalline pattern by X-Ray diffractometer showed an A-type pattern for analyzing starches.
31253275	7	49	theme	irregular	1071:1079	arg1	morphology					1092:1101	the irregular polyhedral morphology	1067:1101	the irregular polyhedral morphology	1067:1101	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	2	50	theme	characteristics	510:524	arg1	terms					464:468	terms	464:468	terms of physicochemical, pasting and thermal characteristics	464:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	2	51	theme	rice	350:353	arg1	cultivars					355:363	these rice cultivars	344:363	these rice cultivars	344:363	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	0	52	theme	structure	40:48	arg1	Effect					0:5	Effect	0:5	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.	0:133	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	7	53	theme	polyhedral	1081:1090	arg1	morphology					1092:1101	the irregular polyhedral morphology	1067:1101	the irregular polyhedral morphology	1067:1101	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	7	54	theme	angular	1147:1153	arg1	morphology					1162:1171	a polyhedral angular shaped morphology	1134:1171	a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1134:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	1	55	from	effect	187:192	arg1	characteristics					311:325	their functional characteristics	294:325	their functional characteristics	294:325	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	4	56	theme	samples	726:732	arg1	values					696:701	The color values	686:701	The color values of the analyzed starch samples	686:732	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	6	57	theme	Crystalline	947:957	arg1	pattern					959:965	Crystalline pattern	947:965	Crystalline pattern by X-Ray diffractometer	947:989	Crystalline pattern by X-Ray diffractometer showed an A-type pattern for analyzing starches.
31253275	3	58	theme	median	646:651	arg1	size					662:665	their median particle size	640:665	their median particle size	640:665	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	4	59	theme	high	746:749	arg1	degree					751:756	a high degree	744:756	a high degree of whiteness and purity	744:780	The color values of the analyzed starch samples indicated a high degree of whiteness and purity.
31253275	7	60	contain	having	1127:1132	arg2	morphology					1162:1171	a polyhedral angular shaped morphology	1134:1171	a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1134:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	7	60	contain	having	1127:1132	arg1	granules					1118:1125	spherical granules	1108:1125	spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface	1108:1219	The starch granules showed the irregular polyhedral morphology with spherical granules having a polyhedral angular shaped morphology packed tightly with a relatively smooth surface.
31253275	3	61	theme	particle	653:660	arg1	size					662:665	their median particle size	640:665	their median particle size	640:665	The diameter of the analyzed starch particles depicted a considerable range varying from 5.139 μm to 8.453 μm as their median particle size visualized at ×50.
31253275	1	62	theme	granule	219:225	arg1	morphology					227:236	granule morphology	219:236	granule morphology	219:236	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	0	63	theme	form	66:69	arg1	Effect					0:5	Effect	0:5	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.	0:133	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	1	64	theme	present	139:145	arg1	research					147:154	The present research	135:154	The present research	135:154	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	1	65	theme	morphology	227:236	arg1	effect					187:192	the effect	183:192	the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics	183:325	The present research was carried out to analyze the effect of chemical composition, granule morphology and crystalline structure of pigmented rice starches on their functional characteristics.
31253275	0	66	theme	crystalline	54:64	arg1	form					66:69	crystalline form	54:69	crystalline form	54:69	Effect of chemical composition, granule structure and crystalline form of pigmented rice starches on their functional characteristics.
31253275	2	67	from	cultivars	451:459	arg1	terms					464:468	terms	464:468	terms of physicochemical, pasting and thermal characteristics	464:524	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31253275	2	68	theme	non-pigmented	437:449	arg1	cultivars					451:459	non-pigmented cultivars	437:459	non-pigmented cultivars	437:459	The starches of these rice cultivars were observed to possess novel characteristic as compared to hybrids or non-pigmented cultivars in terms of physicochemical, pasting and thermal characteristics.
31227155	4	0	theme	bran	911:914	arg1	varieties					916:924	all wheat bran varieties	901:924	all wheat bran varieties	901:924	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	0	1	from	characterization	14:29	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	4	2	theme	glucuronic	797:806	arg1	acid					808:811	glucuronic acid	797:811	glucuronic acid	797:811	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	2	theme	glucuronic	797:806	arg1	rhamnose					745:752	rhamnose	745:752	rhamnose	745:752	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	2	3	from	rich	421:424	arg1	content					437:443	protein content	429:443	protein content (12.45-16.97%)	429:458	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	2	3	from	rich	421:424	arg1	%					457:457	12.45-16.97%	446:457	12.45-16.97%	446:457	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	1	4	theme	alkali	221:226	arg1	Hemi-A					245:250	water-extractable (WE) and alkali extractable (AE) Hemi-A	194:250	Hemi-A	245:250	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	5	theme	fractions	289:297	arg1	composition					179:189	sugar composition	173:189	sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	173:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	5	theme	fractions	289:297	arg1	relationship					352:363	their relationship	346:363	their relationship with dough rheology	346:383	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	5	6	theme	viscoelastic	979:990	arg1	dough					992:996	a viscoelastic dough	977:996	a viscoelastic dough with high G' and G" values	977:1023	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	4	7	theme	fractions	612:620	arg1	composition					597:607	Sugar composition	591:607	Sugar composition	591:607	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	1	8	theme	extractable	228:238	arg1	Hemi-A					245:250	water-extractable (WE) and alkali extractable (AE) Hemi-A	194:250	Hemi-A	245:250	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	4	9	theme	typical	833:839	arg1	arabinoxylan					841:852	the typical arabinoxylan	829:852	the typical arabinoxylan containing arabinose	829:873	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	0	10	from	Isolation	0:8	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	5	11	theme	HD-2947	939:945	arg1	varieties					953:961	HD-3086 and HD-2947 wheat varieties	927:961	varieties	953:961	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	3	12	theme	WE-Hemi-A	474:482	arg1	yield					465:469	The yield	461:469	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo	461:506	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	3	12	theme	WE-Hemi-A	474:482	arg1	lower					513:517	lower	513:517	lower	513:517	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	4	13	attach	present	890:896	arg2	galactose					755:763	galactose	755:763	galactose	755:763	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg2	xylose					879:884	xylose	879:884	xylose	879:884	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg2	acid					808:811	glucuronic acid	797:811	glucuronic acid	797:811	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg2	glucose					766:772	glucose	766:772	glucose	766:772	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg1	varieties					916:924	all wheat bran varieties	901:924	all wheat bran varieties	901:924	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg2	acid					788:791	galacturonic acid	775:791	galacturonic acid	775:791	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	13	attach	present	890:896	arg2	rhamnose					745:752	rhamnose	745:752	rhamnose	745:752	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	14	theme	wheat	905:909	arg1	varieties					916:924	all wheat bran varieties	901:924	all wheat bran varieties	901:924	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	0	15	theme	contribution	83:94	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	15	theme	contribution	83:94	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	15	theme	contribution	83:94	arg1	study					68:72	study	68:72	study of their contribution to wheat flour dough rheology	68:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	16	from	bran	59:62	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	16	from	bran	59:62	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	16	from	bran	59:62	arg1	study					68:72	study	68:72	study of their contribution to wheat flour dough rheology	68:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	16	from	bran	59:62	arg1	arabinoxylans					34:46	arabinoxylans	34:46	arabinoxylans from wheat bran	34:62	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	1	17	theme	AE	241:242	arg1	Hemi-A					245:250	water-extractable (WE) and alkali extractable (AE) Hemi-A	194:250	Hemi-A	245:250	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	0	18	theme	flour	105:109	arg1	dough					111:115	wheat flour dough	99:115	wheat flour dough rheology	99:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	2	19	from	varieties	406:414	arg1	bran					392:395	Wheat bran	386:395	Wheat bran from all varieties	386:414	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	0	20	theme	wheat	99:103	arg1	dough					111:115	wheat flour dough	99:115	wheat flour dough rheology	99:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	2	21	theme	protein	429:435	arg1	content					437:443	protein content	429:443	protein content (12.45-16.97%)	429:458	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	2	21	theme	protein	429:435	arg1	%					457:457	12.45-16.97%	446:457	12.45-16.97%	446:457	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	4	22	from	present	890:896	arg1	varieties					916:924	all wheat bran varieties	901:924	all wheat bran varieties	901:924	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	5	23	theme	G	1015:1015	arg1	values					1018:1023	G" values	1015:1023	G" values	1015:1023	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	4	24	dep	arabinoxylan	841:852	arg1	addition					817:824	addition	817:824	addition	817:824	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	1	25	theme	wheat	326:330	arg1	varieties					332:340	four wheat varieties	321:340	four wheat varieties	321:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	5	26	theme	HD-3086	927:933	arg1	varieties					953:961	HD-3086 and HD-2947 wheat varieties	927:961	varieties	953:961	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	3	27	theme	WE-Oligo	499:506	arg1	yield					465:469	The yield	461:469	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo	461:506	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	3	27	theme	WE-Oligo	499:506	arg1	lower					513:517	lower	513:517	lower	513:517	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	4	28	from	varieties	916:924	arg1	present					890:896	present	890:896	present	890:896	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	29	theme	Sugar	591:595	arg1	composition					597:607	Sugar composition	591:607	Sugar composition	591:607	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	30	theme	anion	653:657	arg1	chromatography					668:681	high-performance anion exchange chromatography	636:681	high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD)	636:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	31	contain	containing	854:863	arg2	arabinose					865:873	arabinose	865:873	arabinose	865:873	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	31	contain	containing	854:863	arg1	arabinoxylan					841:852	the typical arabinoxylan	829:852	the typical arabinoxylan containing arabinose	829:873	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	1	32	theme	Hemi-A	245:250	arg1	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	3	33	theme	WE-Hemi-B	485:493	arg1	yield					465:469	The yield	461:469	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo	461:506	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	3	33	theme	WE-Hemi-B	485:493	arg1	lower					513:517	lower	513:517	lower	513:517	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	1	34	with	relationship	352:363	arg1	rheology					376:383	dough rheology	370:383	dough rheology	370:383	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	35	theme	sugar	173:177	arg1	composition					179:189	sugar composition	173:189	sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	173:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	2	36	theme	Wheat	386:390	arg1	bran					392:395	Wheat bran	386:395	Wheat bran from all varieties	386:414	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	5	37	theme	higher	1082:1087	arg1	percent					1089:1095	a higher percent	1080:1095	a higher percent of AE-Hemi-B	1080:1108	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	0	38	theme	dough	111:115	arg1	rheology					117:124	wheat flour dough rheology	99:124	wheat flour dough rheology	99:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	1	39	theme	varieties	332:340	arg1	bran					313:316	bran	313:316	bran of four wheat varieties	313:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	4	40	theme	pulsed	688:693	arg1	HPAEC-PAD					718:726	HPAEC-PAD	718:726	HPAEC-PAD	718:726	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	40	theme	pulsed	688:693	arg1	detector					708:715	pulsed amperometric detector	688:715	pulsed amperometric detector (HPAEC-PAD)	688:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	5	41	theme	good	1040:1043	arg1	characteristics					1058:1072	good dough-making characteristics	1040:1072	good dough-making characteristics	1040:1072	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	1	42	theme	Hemi-B	253:258	arg1	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	5	43	theme	high	1003:1006	arg1	G					1008:1008	high G'	1003:1009	high G'	1003:1009	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	1	44	dep	yield	163:167	arg1	composition					179:189	sugar composition	173:189	sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	173:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	44	dep	yield	163:167	arg1	relationship					352:363	their relationship	346:363	their relationship with dough rheology	346:383	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	5	45	theme	AE-Hemi-B	1100:1108	arg1	percent					1089:1095	a higher percent	1080:1095	a higher percent of AE-Hemi-B	1080:1108	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	0	46	theme	arabinoxylans	34:46	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	46	theme	arabinoxylans	34:46	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	0	46	theme	arabinoxylans	34:46	arg1	study					68:72	study	68:72	study of their contribution to wheat flour dough rheology	68:124	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	5	47	theme	dough-making	1045:1056	arg1	characteristics					1058:1072	good dough-making characteristics	1040:1072	good dough-making characteristics	1040:1072	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	1	48	with	composition	179:189	arg1	rheology					376:383	dough rheology	370:383	dough rheology	370:383	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	0	49	from	study	68:72	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	5	50	theme	wheat	947:951	arg1	varieties					953:961	HD-3086 and HD-2947 wheat varieties	927:961	varieties	953:961	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	5	51	contain	had	1076:1078	arg2	percent					1089:1095	a higher percent	1080:1095	a higher percent of AE-Hemi-B	1080:1108	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	5	51	contain	had	1076:1078	arg1	varieties					953:961	HD-3086 and HD-2947 wheat varieties	927:961	varieties	953:961	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	5	51	contain	had	1076:1078	arg2	WE-Oligo					1119:1126	WE-Oligo	1119:1126	WE-Oligo	1119:1126	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	1	52	theme	water-extractable	194:210	arg1	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	53	theme	oligosaccharides	264:279	arg1	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	3	54	theme	AE-Oligo	562:569	arg1	yield					528:532	the yield	524:532	the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo	524:569	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	3	55	theme	AE-Hemi-B	548:556	arg1	yield					528:532	the yield	524:532	the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo	524:569	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	4	56	theme	galacturonic	775:786	arg1	acid					788:791	galacturonic acid	775:791	galacturonic acid	775:791	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	56	theme	galacturonic	775:786	arg1	rhamnose					745:752	rhamnose	745:752	rhamnose	745:752	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	57	theme	high-performance	636:651	arg1	chromatography					668:681	high-performance anion exchange chromatography	636:681	high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD)	636:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	0	58	theme	wheat	53:57	arg1	bran					59:62	wheat bran	53:62	wheat bran	53:62	Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology.
31227155	1	59	theme	Oligo	282:286	arg1	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	60	theme	dough	370:374	arg1	rheology					376:383	dough rheology	370:383	dough rheology	370:383	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	4	61	theme	exchange	659:666	arg1	chromatography					668:681	high-performance anion exchange chromatography	636:681	high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD)	636:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	62	theme	amperometric	695:706	arg1	HPAEC-PAD					718:726	HPAEC-PAD	718:726	HPAEC-PAD	718:726	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	62	theme	amperometric	695:706	arg1	detector					708:715	pulsed amperometric detector	688:715	pulsed amperometric detector (HPAEC-PAD)	688:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	2	63	from	content	437:443	arg1	rich					421:424	rich	421:424	rich	421:424	Wheat bran from all varieties were rich in protein content (12.45-16.97%).
31227155	1	64	dep	water-extractable	194:210	arg1	WE					213:214	WE	213:214	WE	213:214	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	65	attach	isolated	299:306	arg1	bran					313:316	bran	313:316	bran of four wheat varieties	313:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	1	65	attach	isolated	299:306	arg2	fractions					289:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions	194:297	water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties	194:340	The study was aimed to evaluate the yield and sugar composition of water-extractable (WE) and alkali extractable (AE) Hemi-A, Hemi-B and oligosaccharides (Oligo) fractions isolated from bran of four wheat varieties and their relationship with dough rheology.
31227155	5	66	theme	wheat	1163:1167	arg1	varieties					1169:1177	C-306 and HS-490 wheat varieties	1146:1177	C-306 and HS-490 wheat varieties	1146:1177	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	4	67	located	present	890:896	arg2	galactose					755:763	galactose	755:763	galactose	755:763	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg2	xylose					879:884	xylose	879:884	xylose	879:884	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg2	acid					808:811	glucuronic acid	797:811	glucuronic acid	797:811	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg2	glucose					766:772	glucose	766:772	glucose	766:772	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg1	varieties					916:924	all wheat bran varieties	901:924	all wheat bran varieties	901:924	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg2	acid					788:791	galacturonic acid	775:791	galacturonic acid	775:791	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	67	located	present	890:896	arg2	rhamnose					745:752	rhamnose	745:752	rhamnose	745:752	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	68	with	chromatography	668:681	arg1	HPAEC-PAD					718:726	HPAEC-PAD	718:726	HPAEC-PAD	718:726	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	4	68	with	chromatography	668:681	arg1	detector					708:715	pulsed amperometric detector	688:715	pulsed amperometric detector (HPAEC-PAD)	688:727	Sugar composition of fractions determined by high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), showed sugars: rhamnose, galactose, glucose, galacturonic acid and glucuronic acid, in addition to the typical arabinoxylan containing arabinose and xylose are present in all wheat bran varieties.
31227155	5	69	dep	yielded	969:975	arg1	indicative					1026:1035	indicative	1026:1035	indicative	1026:1035	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	3	70	theme	AE-Hemi-A	537:545	arg1	yield					528:532	the yield	524:532	the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo	524:569	The yield of WE-Hemi-A, WE-Hemi-B and WE-Oligo were lower than the yield of AE-Hemi-A, AE-Hemi-B and AE-Oligo, for all varieties.
31227155	5	71	theme	"	1016:1016	arg1	values					1018:1023	G" values	1015:1023	G" values	1015:1023	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
31227155	5	72	theme	characteristics	1058:1072	arg1	indicative					1026:1035	indicative	1026:1035	indicative	1026:1035	HD-3086 and HD-2947 wheat varieties which yielded a viscoelastic dough with high G' and G" values (indicative of good dough-making characteristics), had a higher percent of AE-Hemi-B and both WE-Oligo and AE-Oligo than C-306 and HS-490 wheat varieties.
30857965	4	0	theme	aqueous	640:646	arg1	solutions					648:656	aqueous solutions	640:656	aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones	640:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	6	1	theme	reported	1279:1286	arg1	studies					1288:1294	other reported studies	1273:1294	other reported studies	1273:1294	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	7	2	theme	high	1399:1402	arg1	efficiency					1404:1413	high efficiency	1399:1413	high efficiency even after four consecutive reaction runs	1399:1455	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	0	3	contain	have	52:55	arg1	materials					42:50	the porous chitosan-based composite materials	6:50	the porous chitosan-based composite materials	6:50	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	0	3	contain	have	52:55	arg2	chance					59:64	a chance	57:64	a chance	57:64	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	3	4	theme	metal-loaded	458:469	arg1	biosorbents					471:481	the metal-loaded biosorbents	454:481	the metal-loaded biosorbents in further environmental applications, particularly	454:533	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	1	5	theme	wastewaters	224:234	arg1	remediation					209:219	the low-cost remediation	196:219	the low-cost remediation of wastewaters contaminated with metal ions	196:263	Currently, biosorption is considered a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions.
30857965	0	6	theme	LIFE	76:79	arg1	"					80:80	a "NEW LIFE"	69:80	a "NEW LIFE" after Cu(II) ion binding	69:105	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	7	7	dep	along	1358:1362	arg1	with					1364:1367	with	1364:1367	with	1364:1367	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	6	8	theme	rate	1081:1084	arg1	higher					1113:1118	higher	1113:1118	higher	1113:1118	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	6	8	theme	rate	1081:1084	arg1	constant					1086:1093	the apparent degradation rate constant	1056:1093	the apparent degradation rate constant	1056:1093	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	0	9	theme	NEW	72:74	arg1	"					80:80	a "NEW LIFE"	69:80	a "NEW LIFE" after Cu(II) ion binding	69:105	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	7	10	theme	stability	1385:1393	arg1	efficiency					1404:1413	high efficiency	1399:1413	high efficiency even after four consecutive reaction runs	1399:1455	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	7	10	theme	stability	1385:1393	arg1	preparation					1317:1327	The straightforward preparation	1297:1327	The straightforward preparation of copper-loaded composites, along with their excellent stability	1297:1393	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	3	11	from	biosorbents	471:481	arg1	applications					508:519	further environmental applications	486:519	further environmental applications	486:519	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	5	12	theme	co-catalyst	857:867	arg1	absence					842:848	the absence	838:848	the absence of any co-catalyst	838:867	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	3	13	theme	oxidative	544:552	arg1	catalysis					554:562	the oxidative catalysis	540:562	the oxidative catalysis of dyes	540:570	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	6	14	theme	co-catalyst	1216:1226	arg1	content					1205:1211	the content	1201:1211	the content of co-catalyst (H2O2)	1201:1233	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	3	15	theme	waste	423:427	arg1	"					441:441	"waste minimization"	422:441	"waste minimization"	422:441	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	3	16	theme	further	486:492	arg1	applications					508:519	further environmental applications	486:519	further environmental applications	486:519	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	5	17	theme	DE	877:878	arg1	value					880:884	a DE value	875:884	a DE value of 95.3%	875:893	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	4	18	theme	Methyl	618:623	arg1	MO					633:634	MO	633:634	MO	633:634	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	4	18	theme	Methyl	618:623	arg1	Orange					625:630	Methyl Orange	618:630	Methyl Orange (MO)	618:635	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	7	19	theme	potential	1494:1502	arg1	catalysts					1504:1512	potential catalysts	1494:1512	potential catalysts for dye removal by oxidative decolourisation treatments	1494:1568	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	3	20	theme	environmental	494:506	arg1	applications					508:519	further environmental applications	486:519	further environmental applications	486:519	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	7	21	theme	excellent	1375:1383	arg1	stability					1385:1393	their excellent stability	1369:1393	their excellent stability	1369:1393	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	4	22	theme	copper-imprinted	684:699	arg1	composites					716:725	copper-imprinted chitosan-based composites	684:725	copper-imprinted chitosan-based composites	684:725	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	5	23	theme	ion-imprinted	915:927	arg1	catalysts					929:937	the ion-imprinted catalysts	911:937	the ion-imprinted catalysts within 360 min of reaction	911:964	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	1	24	theme	metal	254:258	arg1	ions					260:263	metal ions	254:263	metal ions	254:263	Currently, biosorption is considered a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions.
30857965	4	25	theme	ambient	664:670	arg1	light					672:676	ambient light	664:676	ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones	664:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	2	26	theme	safe	279:282	arg1	issue					344:348	a challenging issue	330:348	a challenging issue	330:348	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	2	26	theme	safe	279:282	arg1	disposal					284:291	the safe disposal	275:291	the safe disposal of metal-loaded biosorbents	275:319	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	4	27	theme	Orange	625:630	arg1	DE					611:612	DE	611:612	DE	611:612	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	4	27	theme	Orange	625:630	arg1	efficiency					599:608	the decolourisation efficiency	579:608	the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones	579:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	0	28	theme	chitosan-based	17:30	arg1	materials					42:50	the porous chitosan-based composite materials	6:50	the porous chitosan-based composite materials	6:50	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	7	29	theme	copper-loaded	1332:1344	arg1	composites					1346:1355	copper-loaded composites	1332:1355	copper-loaded composites	1332:1355	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	5	30	theme	%	893:893	arg1	value					880:884	a DE value	875:884	a DE value of 95.3%	875:893	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	5	31	theme	reaction	957:964	arg1	360 min					946:952	360 min	946:952	360 min of reaction	946:964	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	5	32	theme	MO	798:799	arg1	degradation					801:811	The MO degradation	794:811	The MO degradation	794:811	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	0	33	theme	porous	10:15	arg1	materials					42:50	the porous chitosan-based composite materials	6:50	the porous chitosan-based composite materials	6:50	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	0	34	theme	Cu	88:89	arg1	binding					99:105	Cu(II) ion binding	88:105	Cu(II) ion binding	88:105	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	7	35	theme	ion-imprinted	1469:1481	arg1	systems					1483:1489	our ion-imprinted systems	1465:1489	our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments	1465:1568	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	4	36	theme	chitosan-based	701:714	arg1	composites					716:725	copper-imprinted chitosan-based composites	684:725	copper-imprinted chitosan-based composites	684:725	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	1	37	theme	leading-edge	147:158	arg1	method					185:190	a leading-edge environmentally-friendly method	145:190	a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions	145:263	Currently, biosorption is considered a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions.
30857965	3	38	theme	major	372:376	arg1	objective					378:386	our major objective	368:386	our major objective	368:386	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	6	39	theme	apparent	1060:1067	arg1	higher					1113:1118	higher	1113:1118	higher	1113:1118	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	6	39	theme	apparent	1060:1067	arg1	constant					1086:1093	the apparent degradation rate constant	1056:1093	the apparent degradation rate constant	1056:1093	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	3	40	theme	dyes	567:570	arg1	catalysis					554:562	the oxidative catalysis	540:562	the oxidative catalysis of dyes	540:570	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	4	41	from	efficiency	599:608	arg1	solutions					648:656	aqueous solutions	640:656	aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones	640:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	1	42	theme	environmentally-friendly	160:183	arg1	method					185:190	a leading-edge environmentally-friendly method	145:190	a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions	145:263	Currently, biosorption is considered a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions.
30857965	0	43	theme	composite	32:40	arg1	materials					42:50	the porous chitosan-based composite materials	6:50	the porous chitosan-based composite materials	6:50	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	5	44	theme	non-imprinted	1006:1018	arg1	ones					1020:1023	the non-imprinted ones	1002:1023	the non-imprinted ones	1002:1023	The MO degradation was established first in the absence of any co-catalyst, when a DE value of 95.3% was achieved by the ion-imprinted catalysts within 360 min of reaction, compared to only 67.4% attained by the non-imprinted ones.
30857965	4	45	theme	decolourisation	583:597	arg1	DE					611:612	DE	611:612	DE	611:612	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	4	45	theme	decolourisation	583:597	arg1	efficiency					599:608	the decolourisation efficiency	579:608	the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones	579:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	6	46	theme	other	1273:1277	arg1	studies					1288:1294	other reported studies	1273:1294	other reported studies	1273:1294	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	7	47	theme	oxidative	1533:1541	arg1	treatments					1559:1568	oxidative decolourisation treatments	1533:1568	oxidative decolourisation treatments	1533:1568	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	6	48	theme	degradation	1069:1079	arg1	higher					1113:1118	higher	1113:1118	higher	1113:1118	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	6	48	theme	degradation	1069:1079	arg1	constant					1086:1093	the apparent degradation rate constant	1056:1093	the apparent degradation rate constant	1056:1093	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	7	49	theme	decolourisation	1543:1557	arg1	treatments					1559:1568	oxidative decolourisation treatments	1533:1568	oxidative decolourisation treatments	1533:1568	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	0	50	theme	ion	95:97	arg1	binding					99:105	Cu(II) ion binding	88:105	Cu(II) ion binding	88:105	Could the porous chitosan-based composite materials have a chance to a "NEW LIFE" after Cu(II) ion binding?
30857965	7	51	theme	reaction	1443:1450	arg1	runs					1452:1455	four consecutive reaction runs	1426:1455	four consecutive reaction runs	1426:1455	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	2	52	theme	biosorbents	309:319	arg1	issue					344:348	a challenging issue	330:348	a challenging issue	330:348	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	2	52	theme	biosorbents	309:319	arg1	disposal					284:291	the safe disposal	275:291	the safe disposal of metal-loaded biosorbents	275:319	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	7	53	theme	dye	1518:1520	arg1	removal					1522:1528	dye removal	1518:1528	dye removal by oxidative decolourisation treatments	1518:1568	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	7	54	theme	composites	1346:1355	arg1	efficiency					1404:1413	high efficiency	1399:1413	high efficiency even after four consecutive reaction runs	1399:1455	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	7	54	theme	composites	1346:1355	arg1	preparation					1317:1327	The straightforward preparation	1297:1327	The straightforward preparation of copper-loaded composites, along with their excellent stability	1297:1393	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	2	55	theme	metal-loaded	296:307	arg1	biosorbents					309:319	metal-loaded biosorbents	296:319	metal-loaded biosorbents	296:319	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	7	56	theme	straightforward	1301:1315	arg1	preparation					1317:1327	The straightforward preparation	1297:1327	The straightforward preparation of copper-loaded composites, along with their excellent stability	1297:1393	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30857965	4	57	theme	non-imprinted	744:756	arg1	ones					758:761	non-imprinted ones	744:761	non-imprinted ones	744:761	Thus, the decolourisation efficiency (DE) of Methyl Orange (MO) in aqueous solutions under ambient light using copper-imprinted chitosan-based composites in comparison to non-imprinted ones was investigated in this work.
30857965	3	58	theme	"	441:441	arg1	possibility					407:417	the possibility	403:417	the possibility of "waste minimization"	403:441	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	3	59	theme	minimization	429:440	arg1	"					441:441	"waste minimization"	422:441	"waste minimization"	422:441	In this context, our major objective was to explore the possibility of "waste minimization" by reusing the metal-loaded biosorbents in further environmental applications, particularly into the oxidative catalysis of dyes.
30857965	6	60	theme	Fenton-like	1032:1042	arg1	conditions					1044:1053	Fenton-like conditions	1032:1053	Fenton-like conditions	1032:1053	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	6	61	dep	higher	1113:1118	arg1	whereas					1193:1199	whereas	1193:1199	whereas	1193:1199	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	6	61	dep	higher	1113:1118	arg1	increasing					1128:1137	increasing	1128:1137	increasing within 40 min to about 98.6%, and 70.5% respectively	1128:1190	Under Fenton-like conditions, the apparent degradation rate constant was seventy times higher, the DE increasing within 40 min to about 98.6%, and 70.5% respectively, whereas the content of co-catalyst (H2O2) was significantly lowered compared to other reported studies.
30857965	1	62	theme	low-cost	200:207	arg1	remediation					209:219	the low-cost remediation	196:219	the low-cost remediation of wastewaters contaminated with metal ions	196:263	Currently, biosorption is considered a leading-edge environmentally-friendly method for the low-cost remediation of wastewaters contaminated with metal ions.
30857965	2	63	theme	challenging	332:342	arg1	disposal					284:291	the safe disposal	275:291	the safe disposal of metal-loaded biosorbents	275:319	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	2	63	theme	challenging	332:342	arg1	issue					344:348	a challenging issue	330:348	a challenging issue	330:348	However, the safe disposal of metal-loaded biosorbents is still a challenging issue.
30857965	7	64	theme	consecutive	1431:1441	arg1	runs					1452:1455	four consecutive reaction runs	1426:1455	four consecutive reaction runs	1426:1455	The straightforward preparation of copper-loaded composites, along with their excellent stability and high efficiency even after four consecutive reaction runs support our ion-imprinted systems as potential catalysts for dye removal by oxidative decolourisation treatments.
30707999	8	0	theme	certain	1334:1340	arg1	extent					1342:1347	certain extent	1334:1347	certain extent of transformation of the crystalline drug to its amorphous form	1334:1411	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	10	1	theme	ZIP	1567:1569	arg1	delivery					1571:1578	gastroretentive ZIP delivery	1551:1578	gastroretentive ZIP delivery over an extended period of time	1551:1610	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	4	2	theme	mucin	854:858	arg1	ability					871:877	mucin adsorption ability	854:877	mucin adsorption ability	854:877	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	3	3	dep	DGP	680:682	arg1	AG					684:685	AG	684:685	AG	684:685	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	1	4	theme	modified	180:187	arg1	composites					211:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	8	5	theme	thermal	1228:1234	arg1	X-ray					1237:1241	the thermal, X-ray and infrared analyses	1224:1263	X-ray	1237:1241	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	10	6	theme	gastroretentive	1551:1565	arg1	delivery					1571:1578	gastroretentive ZIP delivery	1551:1578	gastroretentive ZIP delivery over an extended period of time	1551:1610	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	3	7	theme	filler	708:713	arg1	existence					721:729	filler (MMT) existence	708:729	filler (MMT) existence	708:729	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	1	8	theme	montmorillonite	189:203	arg1	composites					211:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	9	9	theme	SEM	1418:1420	arg1	studies					1422:1428	The SEM studies	1414:1428	The SEM studies	1414:1428	The SEM studies revealed the spherical morphology of the composites.
30707999	2	10	theme	ionotropic	397:406	arg1	protocol					417:424	ionotropic gelation protocol	397:424	ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA)	397:519	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	8	11	theme	transformation	1352:1365	arg1	extent					1342:1347	certain extent	1334:1347	certain extent of transformation of the crystalline drug to its amorphous form	1334:1411	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	4	12	theme	optimal	763:769	arg1	F-3					783:785	F-3	783:785	F-3	783:785	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	4	12	theme	optimal	763:769	arg1	composites					771:780	The optimal composites	759:780	The optimal composites (F-3)	759:786	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	2	13	theme	clay-biopolymer	351:365	arg1	matrices					367:374	The ZIP-loaded clay-biopolymer matrices	336:374	The ZIP-loaded clay-biopolymer matrices	336:374	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	3	14	theme	crosslinking	735:746	arg1	procedure					748:756	crosslinking procedure	735:756	crosslinking procedure	735:756	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	4	15	theme	adsorption	860:869	arg1	ability					871:877	mucin adsorption ability	854:877	mucin adsorption ability	854:877	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	2	16	theme	gelation	408:415	arg1	protocol					417:424	ionotropic gelation protocol	397:424	ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA)	397:519	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	10	17	theme	extended	1588:1595	arg1	period					1597:1602	an extended period	1585:1602	an extended period of time	1585:1610	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	8	18	theme	drug	1303:1306	arg1	environment					1288:1298	a compatible environment	1275:1298	a compatible environment of drug in the composites	1275:1324	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	1	19	theme	Novel	108:112	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	19	theme	Novel	108:112	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	8	20	theme	infrared	1247:1254	arg1	analyses					1256:1263	the thermal, X-ray and infrared analyses	1224:1263	analyses	1256:1263	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	9	21	theme	composites	1471:1480	arg1	morphology					1453:1462	the spherical morphology	1439:1462	the spherical morphology of the composites	1439:1480	The SEM studies revealed the spherical morphology of the composites.
30707999	4	22	theme	%	825:825	arg1	Q8h					816:818	Q8h	816:818	Q8h	816:818	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	4	22	theme	%	825:825	arg1	%					825:825	52%	823:825	52%	823:825	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	4	22	theme	%	825:825	arg1	%					810:810	61%	808:810	61%	808:810	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	1	23	theme	diethanolamine-grafted	114:135	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	23	theme	diethanolamine-grafted	114:135	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	24	theme	MMT	206:208	arg1	composites					211:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	0	25	theme	arabic	19:24	arg1	gum					26:28	Ziprasidone-loaded arabic gum	0:28	Ziprasidone-loaded arabic gum	0:28	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	7	26	theme	molar	1089:1093	arg1	mass					1095:1098	The molar mass	1085:1098	The molar mass between crosslinks of composites (F-3) calculated employing Flory-Rehner equation	1085:1180	The molar mass between crosslinks of composites (F-3) calculated employing Flory-Rehner equation was increased with temperature.
30707999	2	27	theme	ZIP-loaded	340:349	arg1	matrices					367:374	The ZIP-loaded clay-biopolymer matrices	336:374	The ZIP-loaded clay-biopolymer matrices	336:374	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	1	28	theme	high-methoxyl	137:149	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	28	theme	high-methoxyl	137:149	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	0	29	theme	Ziprasidone-loaded	0:17	arg1	gum					26:28	Ziprasidone-loaded arabic gum	0:28	Ziprasidone-loaded arabic gum	0:28	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	1	30	theme	floating	298:305	arg1	mechanisms					324:333	floating and mucoadhesion mechanisms	298:333	floating and mucoadhesion mechanisms	298:333	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	4	31	theme	outstanding	832:842	arg1	buoyancy					844:851	outstanding buoyancy	832:851	outstanding buoyancy	832:851	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	1	32	theme	pectin	151:156	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	32	theme	pectin	151:156	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	2	33	theme	zinc	436:439	arg1	acetate					441:447	zinc acetate	436:447	zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA)	436:519	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	2	34	theme	crosslinker	488:498	arg1	absence					468:474	absence	468:474	absence	468:474	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	2	34	theme	crosslinker	488:498	arg1	presence					456:463	presence	456:463	presence	456:463	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	5	35	theme	driven	997:1002	arg1	mechanism					1004:1012	Fickian diffusion driven mechanism	979:1012	Fickian diffusion driven mechanism	979:1012	The release profile of F-3 was best fitted in the Korsmeyer-Peppas model with Fickian diffusion driven mechanism.
30707999	7	36	theme	composites	1122:1131	arg1	crosslinks					1108:1117	crosslinks	1108:1117	crosslinks of composites (F-3) calculated employing Flory-Rehner equation	1108:1180	The molar mass between crosslinks of composites (F-3) calculated employing Flory-Rehner equation was increased with temperature.
30707999	9	37	theme	spherical	1443:1451	arg1	morphology					1453:1462	the spherical morphology	1439:1462	the spherical morphology of the composites	1439:1480	The SEM studies revealed the spherical morphology of the composites.
30707999	3	38	dep	efficiency	579:588	arg1	%					596:596	%	596:596	%	596:596	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	3	38	dep	efficiency	579:588	arg1	DEE					591:593	DEE	591:593	DEE	591:593	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	8	39	theme	compatible	1277:1286	arg1	environment					1288:1298	a compatible environment	1275:1298	a compatible environment of drug in the composites	1275:1324	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	2	40	theme	covalent	479:486	arg1	glutaraldehyde					501:514	glutaraldehyde	501:514	glutaraldehyde (GA)	501:519	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	2	40	theme	covalent	479:486	arg1	crosslinker					488:498	covalent crosslinker	479:498	covalent crosslinker	479:498	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	1	41	theme	mucoadhesion	311:322	arg1	mechanisms					324:333	floating and mucoadhesion mechanisms	298:333	floating and mucoadhesion mechanisms	298:333	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	10	42	theme	developed	1499:1507	arg1	appropriate					1535:1545	appropriate	1535:1545	appropriate	1535:1545	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	10	42	theme	developed	1499:1507	arg1	composites					1520:1529	the newly developed DGP-AG-MMT composites	1489:1529	the newly developed DGP-AG-MMT composites	1489:1529	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	8	43	theme	amorphous	1398:1406	arg1	form					1408:1411	its amorphous form	1394:1411	its amorphous form	1394:1411	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	1	44	theme	DGP	159:161	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	44	theme	DGP	159:161	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	0	45	theme	pectin	67:72	arg1	composites					96:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	3	46	theme	excellent	553:561	arg1	efficiency					579:588	excellent drug entrapment efficiency	553:588	excellent drug entrapment efficiency (DEE, %)	553:597	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	4	47	theme	Q8h	816:818	arg1	DEE					801:803	DEE	801:803	DEE of 61% and Q8h of 52%	801:825	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	6	48	theme	Freundlich	1063:1072	arg1	isotherms					1074:1082	Freundlich isotherms	1063:1082	Freundlich isotherms	1063:1082	The mucin adsorption to composites F-3 followed Freundlich isotherms.
30707999	0	49	theme	montmorillonite-tailor-made	39:65	arg1	composites					96:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	7	50	theme	Flory-Rehner	1160:1171	arg1	equation					1173:1180	Flory-Rehner equation	1160:1180	Flory-Rehner equation	1160:1180	The molar mass between crosslinks of composites (F-3) calculated employing Flory-Rehner equation was increased with temperature.
30707999	2	51	dep	presence	456:463	arg1	the					452:454	the	452:454	the	452:454	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	2	52	from	acetate	441:447	arg1	absence					468:474	absence	468:474	absence	468:474	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	2	52	from	acetate	441:447	arg1	presence					456:463	presence	456:463	presence	456:463	The ZIP-loaded clay-biopolymer matrices were accomplished by ionotropic gelation protocol utilizing zinc acetate in the presence or absence of covalent crosslinker, glutaraldehyde (GA).
30707999	1	53	theme	intragastric	241:252	arg1	delivery					276:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	0	54	theme	gastroretentive	80:94	arg1	composites					96:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	3	55	theme	drug	563:566	arg1	efficiency					579:588	excellent drug entrapment efficiency	553:588	excellent drug entrapment efficiency (DEE, %)	553:597	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	8	56	theme	drug	1386:1389	arg1	transformation					1352:1365	transformation	1352:1365	transformation of the crystalline drug to its amorphous form	1352:1411	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	10	57	theme	DGP-AG-MMT	1509:1518	arg1	appropriate					1535:1545	appropriate	1535:1545	appropriate	1535:1545	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	10	57	theme	DGP-AG-MMT	1509:1518	arg1	composites					1520:1529	the newly developed DGP-AG-MMT composites	1489:1529	the newly developed DGP-AG-MMT composites	1489:1529	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	5	58	theme	Korsmeyer-Peppas	951:966	arg1	model					968:972	the Korsmeyer-Peppas model	947:972	the Korsmeyer-Peppas model	947:972	The release profile of F-3 was best fitted in the Korsmeyer-Peppas model with Fickian diffusion driven mechanism.
30707999	8	59	theme	crystalline	1374:1384	arg1	drug					1386:1389	the crystalline drug	1370:1389	the crystalline drug	1370:1389	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	5	60	theme	release	905:911	arg1	profile					913:919	The release profile	901:919	The release profile of F-3	901:926	The release profile of F-3 was best fitted in the Korsmeyer-Peppas model with Fickian diffusion driven mechanism.
30707999	0	61	theme	based	74:78	arg1	composites					96:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	montmorillonite-tailor-made pectin based gastroretentive composites	39:105	Ziprasidone-loaded arabic gum modified montmorillonite-tailor-made pectin based gastroretentive composites.
30707999	3	62	theme	Various	522:528	arg1	formulations					530:541	Various formulations	522:541	Various formulations	522:541	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	3	63	theme	entrapment	568:577	arg1	efficiency					579:588	excellent drug entrapment efficiency	553:588	excellent drug entrapment efficiency (DEE, %)	553:597	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	1	64	theme	-arabic	163:169	arg1	gum					171:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum	108:173	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	64	theme	-arabic	163:169	arg1	AG					176:177	AG	176:177	AG	176:177	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	3	65	theme	drug	613:616	arg1	profiles					626:633	drug release profiles	613:633	drug release profiles	613:633	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	6	66	theme	mucin	1019:1023	arg1	adsorption					1025:1034	The mucin adsorption	1015:1034	The mucin adsorption to composites F-3	1015:1052	The mucin adsorption to composites F-3 followed Freundlich isotherms.
30707999	1	67	theme	gum	171:173	arg1	composites					211:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites	108:220	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	1	68	theme	ziprasidone	254:264	arg1	delivery					276:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	3	69	theme	polymer-blend	665:677	arg1	ratios					688:693	the polymer-blend (DGP:AG) ratios	661:693	the polymer-blend (DGP:AG) ratios	661:693	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	5	70	theme	F-3	924:926	arg1	profile					913:919	The release profile	901:919	The release profile of F-3	901:926	The release profile of F-3 was best fitted in the Korsmeyer-Peppas model with Fickian diffusion driven mechanism.
30707999	1	71	theme	HCl	266:268	arg1	delivery					276:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
30707999	3	72	dep	polymer-blend	665:677	arg1	DGP					680:682	DGP	680:682	DGP	680:682	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	4	73	theme	%	810:810	arg1	DEE					801:803	DEE	801:803	DEE of 61% and Q8h of 52%	801:825	The optimal composites (F-3) demonstrated DEE of 61% and Q8h of 52% with outstanding buoyancy, mucin adsorption ability and biodegradability.
30707999	8	74	from	environment	1288:1298	arg1	composites					1315:1324	the composites	1311:1324	the composites	1311:1324	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	8	75	theme	X-ray	1237:1241	arg1	analyses					1256:1263	the thermal, X-ray and infrared analyses	1224:1263	analyses	1256:1263	Moreover, the thermal, X-ray and infrared analyses confirmed a compatible environment of drug in the composites, except certain extent of transformation of the crystalline drug to its amorphous form.
30707999	3	76	theme	MMT	716:718	arg1	existence					721:729	filler (MMT) existence	708:729	filler (MMT) existence	708:729	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	10	77	theme	time	1607:1610	arg1	period					1597:1602	an extended period	1585:1602	an extended period of time	1585:1610	Thus, the newly developed DGP-AG-MMT composites are appropriate for gastroretentive ZIP delivery over an extended period of time.
30707999	3	78	theme	release	618:624	arg1	profiles					626:633	drug release profiles	613:633	drug release profiles	613:633	Various formulations exhibited excellent drug entrapment efficiency (DEE, %) and sustained drug release profiles, which were influenced by the polymer-blend (DGP:AG) ratios, reinforcing filler (MMT) existence and crosslinking procedure.
30707999	1	79	theme	ZIP	271:273	arg1	delivery					276:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	intragastric ziprasidone HCl (ZIP) delivery	241:283	Novel diethanolamine-grafted high-methoxyl pectin (DGP)-arabic gum (AG) modified montmorillonite (MMT) composites were developed for intragastric ziprasidone HCl (ZIP) delivery by combining floating and mucoadhesion mechanisms.
31887955	6	0	theme	AV	883:884	arg1	property					859:866	The mechanical property	844:866	The mechanical property of the CO/CSGC@AV	844:884	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	7	1	theme	AV	1058:1059	arg1	presence					1046:1053	the presence	1042:1053	the presence of AV	1042:1059	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	6	2	theme	mechanical	848:857	arg1	property					859:866	The mechanical property	844:866	The mechanical property of the CO/CSGC@AV	844:884	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	3	3	theme	scaffold	455:462	arg1	morphology					342:351	morphology	342:351	morphology	342:351	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	size					382:385	pore size	377:385	pore size	377:385	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	porosity					388:395	porosity	388:395	porosity	388:395	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	behavior					432:439	degradation behavior	420:439	degradation behavior	420:439	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	properties					365:374	mechanical properties	354:374	mechanical properties	354:374	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	ability					407:413	swelling ability	398:413	swelling ability	398:413	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	3	theme	scaffold	455:462	arg1	process					333:339	Extraction process	322:339	Extraction process	322:339	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	4	4	theme	commune	548:554	arg1	mycelium					522:529	mycelium	522:529	mycelium of Schizophyllum commune	522:554	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	0	5	theme	aloe	87:90	arg1	vera					92:95	aloe vera	87:95	aloe vera	87:95	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	6	6	theme	@	882:882	arg1	AV					883:884	the CO/CSGC@AV	871:884	the CO/CSGC@AV	871:884	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	8	7	theme	skin	1150:1153	arg1	regeneration					1155:1166	clinical skin regeneration	1141:1166	clinical skin regeneration	1141:1166	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	1	8	dep	Part	98:101	arg1	characterization					122:137	characterization	122:137	characterization	122:137	Part I: Preparation and characterization.
31887955	1	8	dep	Part	98:101	arg1	Preparation					106:116	Preparation	106:116	Preparation	106:116	Part I: Preparation and characterization.
31887955	4	9	theme	Schizophyllum	534:546	arg1	commune					548:554	Schizophyllum commune	534:554	Schizophyllum commune	534:554	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	5	10	theme	swelling	676:683	arg1	results					665:671	The results	661:671	The results of swelling and hydrolytic degradation studies	661:718	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	6	11	theme	CO/CSGC	875:881	arg1	AV					883:884	the CO/CSGC@AV	871:884	the CO/CSGC@AV	871:884	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	4	12	theme	hollow	561:566	arg1	fiber					568:572	hollow fiber	561:572	hollow fiber	561:572	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	3	13	theme	Extraction	322:331	arg1	process					333:339	Extraction process	322:339	Extraction process	322:339	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	4	14	dep	2.5 ± 0.5 μm	646:657	arg1	of					642:643	of	642:643	of	642:643	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	5	15	theme	concentration-dependent	812:834	arg1	manner					836:841	a concentration-dependent manner	810:841	a concentration-dependent manner	810:841	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	5	16	theme	hydrolytic	689:698	arg1	degradation					700:710	hydrolytic degradation	689:710	hydrolytic degradation	689:710	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	2	17	theme	CO	150:151	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	0	18	with	embedded	73:80	arg1	vera					92:95	aloe vera	87:95	aloe vera	87:95	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	2	19	theme	@	253:253	arg1	AV					254:255	CO/CSGC@AV	246:255	CO/CSGC@AV	246:255	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	19	theme	@	253:253	arg1	scaffold					236:243	aloe vera (AV) dressing scaffold	212:243	aloe vera (AV) dressing scaffold (CO/CSGC@AV)	212:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	4	20	theme	outer	621:625	arg1	2.5 ± 0.5 μm					646:657	2.5 ± 0.5 μm	646:657	2.5 ± 0.5 μm	646:657	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	4	20	theme	outer	621:625	arg1	diameter					633:640	outer fiber diameter	621:640	outer fiber diameter of (2.5 ± 0.5 μm)	621:658	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	5	21	theme	@	768:768	arg1	AV					769:770	CO/CSGC@AV	761:770	CO/CSGC@AV	761:770	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	8	22	theme	clinical	1141:1148	arg1	regeneration					1155:1166	clinical skin regeneration	1141:1166	clinical skin regeneration	1141:1166	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	2	23	theme	CO/CSGC	246:252	arg1	AV					254:255	CO/CSGC@AV	246:255	CO/CSGC@AV	246:255	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	23	theme	CO/CSGC	246:252	arg1	scaffold					236:243	aloe vera (AV) dressing scaffold	212:243	aloe vera (AV) dressing scaffold (CO/CSGC@AV)	212:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	8	24	theme	dressing	1103:1110	arg1	scaffold					1112:1119	the new dressing scaffold	1095:1119	the new dressing scaffold	1095:1119	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	0	25	theme	complex	16:22	arg1	fibers					31:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	2	26	theme	Collagen	140:147	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	7	27	dep	size	979:982	arg1	The					970:972	The	970:972	The	970:972	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	8	28	theme	infected	1186:1193	arg1	wounds					1203:1208	infected chronic wounds	1186:1208	infected chronic wounds	1186:1208	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	0	29	theme	Chitosan-glucan	0:14	arg1	fibers					31:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	8	30	theme	new	1099:1101	arg1	scaffold					1112:1119	the new dressing scaffold	1095:1119	the new dressing scaffold	1095:1119	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	8	31	theme	chronic	1195:1201	arg1	wounds					1203:1208	infected chronic wounds	1186:1208	infected chronic wounds	1186:1208	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	4	32	theme	fiber	627:631	arg1	2.5 ± 0.5 μm					646:657	2.5 ± 0.5 μm	646:657	2.5 ± 0.5 μm	646:657	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	4	32	theme	fiber	627:631	arg1	diameter					633:640	outer fiber diameter	621:640	outer fiber diameter of (2.5 ± 0.5 μm)	621:658	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	6	33	theme	hollow	931:936	arg1	fibers					938:943	CSGC hollow fibers	926:943	CSGC hollow fibers	926:943	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	7	34	theme	AV	1012:1013	arg1	porosity					988:995	porosity	988:995	porosity	988:995	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	7	34	theme	AV	1012:1013	arg1	size					979:982	pore size	974:982	pore size	974:982	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	5	35	theme	CO/CSGC	761:767	arg1	AV					769:770	CO/CSGC@AV	761:770	CO/CSGC@AV	761:770	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	0	36	theme	hollow	24:29	arg1	fibers					31:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers	0:36	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	6	37	theme	CSGC	926:929	arg1	fibers					938:943	CSGC hollow fibers	926:943	CSGC hollow fibers	926:943	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	2	38	theme	CSGC	179:182	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	39	theme	freeze-dried	300:311	arg1	process					313:319	the freeze-dried process	296:319	the freeze-dried process	296:319	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	4	40	theme	of	599:600	arg1	600 ± 250 nm					603:614	600 ± 250 nm	603:614	600 ± 250 nm	603:614	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	4	40	theme	of	599:600	arg1	diameter					590:597	inner diameter of	584:600	inner diameter of (600 ± 250 nm)	584:615	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	0	41	theme	collagen	49:56	arg1	dressing					64:71	collagen wound dressing	49:71	collagen wound dressing embedded with aloe vera	49:95	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	7	42	theme	CO/CSGC	1004:1010	arg1	AV					1012:1013	the CO/CSGC@AV	1000:1013	the CO/CSGC@AV	1000:1013	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	3	43	theme	swelling	398:405	arg1	ability					407:413	swelling ability	398:413	swelling ability	398:413	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	3	44	theme	pore	377:380	arg1	size					382:385	pore size	377:385	pore size	377:385	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	4	45	theme	hollow	488:493	arg1	fibers					495:500	CSGC hollow fibers	483:500	CSGC hollow fibers	483:500	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	6	46	theme	native	959:964	arg1	CO					966:967	native CO	959:967	native CO	959:967	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	2	47	theme	complex	170:176	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	7	48	theme	pore	974:977	arg1	size					979:982	pore size	974:982	pore size	974:982	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	5	49	theme	degradation	700:710	arg1	results					665:671	The results	661:671	The results of swelling and hydrolytic degradation studies	661:718	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	3	50	theme	mechanical	354:363	arg1	properties					365:374	mechanical properties	354:374	mechanical properties	354:374	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	4	51	theme	CSGC	483:486	arg1	fibers					495:500	CSGC hollow fibers	483:500	CSGC hollow fibers	483:500	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	2	52	theme	/chitosan-glucan	153:168	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	53	theme	vera	217:220	arg1	AV					254:255	CO/CSGC@AV	246:255	CO/CSGC@AV	246:255	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	53	theme	vera	217:220	arg1	scaffold					236:243	aloe vera (AV) dressing scaffold	212:243	aloe vera (AV) dressing scaffold (CO/CSGC@AV)	212:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	7	54	theme	@	1011:1011	arg1	AV					1012:1013	the CO/CSGC@AV	1000:1013	the CO/CSGC@AV	1000:1013	The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV.
31887955	0	55	theme	wound	58:62	arg1	dressing					64:71	collagen wound dressing	49:71	collagen wound dressing embedded with aloe vera	49:95	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	2	56	theme	aloe	212:215	arg1	AV					223:224	AV	223:224	AV	223:224	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	56	theme	aloe	212:215	arg1	vera					217:220	aloe vera	212:220	aloe vera (AV) dressing scaffold (CO/CSGC@AV)	212:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	3	57	theme	composites	444:453	arg1	scaffold					455:462	composites scaffold	444:462	composites scaffold	444:462	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	5	58	dep	swelling	676:683	arg1	studies					712:718	studies	712:718	studies	712:718	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	3	59	theme	degradation	420:430	arg1	behavior					432:439	degradation behavior	420:439	degradation behavior	420:439	Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated.
31887955	2	60	theme	hollow	185:190	arg1	fibers					192:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers	140:197	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV)	140:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	61	theme	first	282:286	arg1	time					288:291	the first time	278:291	the first time	278:291	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	62	theme	dressing	227:234	arg1	AV					254:255	CO/CSGC@AV	246:255	CO/CSGC@AV	246:255	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	2	62	theme	dressing	227:234	arg1	scaffold					236:243	aloe vera (AV) dressing scaffold	212:243	aloe vera (AV) dressing scaffold (CO/CSGC@AV)	212:256	Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process.
31887955	0	63	theme	embedded	73:80	arg1	dressing					64:71	collagen wound dressing	49:71	collagen wound dressing embedded with aloe vera	49:95	Chitosan-glucan complex hollow fibers reinforced collagen wound dressing embedded with aloe vera.
31887955	8	64	contain	has	1121:1123	arg1	scaffold					1112:1119	the new dressing scaffold	1095:1119	the new dressing scaffold	1095:1119	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	8	64	contain	has	1121:1123	arg2	potential					1127:1135	a potential	1125:1135	a potential	1125:1135	All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
31887955	5	65	theme	AV	769:770	arg1	physicochemical					742:756	the physicochemical	738:756	the physicochemical of CO/CSGC@AV	738:770	The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner.
31887955	6	66	theme	encapsulated	905:916	arg1	AV					918:919	encapsulated AV	905:919	encapsulated AV into CSGC hollow fibers	905:943	The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO.
31887955	4	67	theme	inner	584:588	arg1	600 ± 250 nm					603:614	600 ± 250 nm	603:614	600 ± 250 nm	603:614	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
31887955	4	67	theme	inner	584:588	arg1	diameter					590:597	inner diameter of	584:600	inner diameter of (600 ± 250 nm)	584:615	CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm).
30506688	4	0	theme	different	965:973	arg1	sources					985:991	different vegetable sources	965:991	different vegetable sources	965:991	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	6	1	theme	particles	1388:1396	arg1	swelling					1372:1379	swelling	1372:1379	swelling of the particles	1372:1396	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	10	2	theme	food	2165:2168	arg1	sources					2170:2176	different food sources	2155:2176	different food sources	2155:2176	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	5	3	from	fractions	1170:1178	arg1	increase					1120:1127	the increase	1116:1127	the increase in the rheological values at high volume fractions of powder	1116:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	5	3	from	fractions	1170:1178	arg1	values					1148:1153	the rheological values	1132:1153	the rheological values at high volume fractions of powder	1132:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	3	4	theme	slight	625:630	arg1	differences					632:642	slight differences	625:642	slight differences in the printability and rheological values	625:685	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	1	5	from	factors	256:262	arg1	systems					119:125	food ink systems	110:125	food ink systems in which the particles are dispersed in a hydrocolloid matrix	110:187	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	4	6	theme	powder	772:777	arg1	content					779:785	the powder content	768:785	the powder content	768:785	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	2	7	theme	hydration	488:496	arg1	properties					498:507	different hydration properties	478:507	different hydration properties	478:507	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	10	8	theme	different	2155:2163	arg1	sources					2170:2176	different food sources	2155:2176	different food sources	2155:2176	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	3	9	from	differences	632:642	arg1	printability					651:662	printability	651:662	printability	651:662	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	3	9	from	differences	632:642	arg1	values					680:685	rheological values	668:685	rheological values	668:685	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	4	10	theme	lowest	835:840	arg1	capacity					858:865	the lowest water hydration capacity	831:865	the lowest water hydration capacity	831:865	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	3	11	dep	different	713:721	arg1	vegetable					723:731	vegetable	723:731	vegetable	723:731	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	5	12	from	differences	1207:1217	arg1	printability					1222:1233	printability	1222:1233	printability	1222:1233	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	3	13	theme	rheological	668:678	arg1	values					680:685	rheological values	668:685	rheological values	668:685	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	8	14	theme	food	1842:1845	arg1	materials					1847:1855	all food materials	1838:1855	all food materials that can be powdered	1838:1876	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	1	15	theme	food	110:113	arg1	systems					119:125	food ink systems	110:125	food ink systems in which the particles are dispersed in a hydrocolloid matrix	110:187	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	7	16	theme	extruded	1532:1539	arg1	hardness					1541:1548	their low extruded hardness	1522:1548	their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg)	1522:1580	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	7	16	theme	extruded	1532:1539	arg1	kg					1578:1579	2.96 ± 0.23 to 3.46 ± 0.16 kg	1551:1579	2.96 ± 0.23 to 3.46 ± 0.16 kg	1551:1579	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	2	17	theme	different	478:486	arg1	properties					498:507	different hydration properties	478:507	different hydration properties	478:507	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	3	18	theme	different	713:721	arg1	sources					733:739	the different vegetable sources	709:739	the different vegetable sources in all hydrocolloids	709:760	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	5	19	theme	water	1033:1037	arg1	capacity					1049:1056	its higher water hydration capacity	1022:1056	its higher water hydration capacity	1022:1056	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	8	20	theme	texturization	1700:1712	arg1	technology					1714:1723	The powder-based texturization technology	1683:1723	The powder-based texturization technology introduced in this study	1683:1748	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	9	21	theme	present	1896:1902	arg1	invention					1904:1912	the present invention	1892:1912	the present invention	1892:1912	In addition, the present invention can be applied to a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing.
30506688	5	22	theme	volume	1163:1168	arg1	fractions					1170:1178	high volume fractions	1158:1178	high volume fractions of powder	1158:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	0	23	theme	Inks	80:83	arg1	Properties					39:48	Rheological Properties	27:48	Rheological Properties	27:48	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	0	23	theme	Inks	80:83	arg1	Printability					54:65	Printability	54:65	Printability of Vegetable Inks for 3D Food Printing	54:104	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	3	24	dep	printability	651:662	arg1	the					647:649	the	647:649	the	647:649	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	7	25	theme	resulting	1591:1599	arg1	objects					1601:1607	the resulting objects	1587:1607	the resulting objects	1587:1607	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	4	26	theme	hydration	848:856	arg1	capacity					858:865	the lowest water hydration capacity	831:865	the lowest water hydration capacity	831:865	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	0	27	from	Effect	0:5	arg1	Properties					39:48	Rheological Properties	27:48	Rheological Properties	27:48	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	0	27	from	Effect	0:5	arg1	Printability					54:65	Printability	54:65	Printability of Vegetable Inks for 3D Food Printing	54:104	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	0	28	theme	3D	89:90	arg1	Printing					97:104	3D Food Printing	89:104	3D Food Printing	89:104	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	1	29	theme	system	325:330	arg1	printability					278:289	printability	278:289	printability	278:289	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	29	theme	system	325:330	arg1	properties					307:316	rheological properties	295:316	rheological properties	295:316	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	2	30	theme	rheological	554:564	arg1	properties					566:575	rheological properties	554:575	rheological properties	554:575	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	5	31	theme	vegetable	1253:1261	arg1	sources					1263:1269	different vegetable sources	1243:1269	different vegetable sources	1243:1269	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	6	32	theme	Confocal	1272:1279	arg1	microscopy					1296:1305	Confocal laser scanning microscopy	1272:1305	Confocal laser scanning microscopy analysis of the vegetable inks	1272:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	0	33	theme	Vegetable	70:78	arg1	Inks					80:83	Vegetable Inks	70:83	Vegetable Inks	70:83	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	8	34	theme	standardized	1761:1772	arg1	method					1774:1779	a standardized method	1759:1779	a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered	1759:1876	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	3	35	from	sources	733:739	arg1	hydrocolloids					748:760	all hydrocolloids	744:760	all hydrocolloids	744:760	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	9	36	theme	printing	1937:1944	arg1	technique					1946:1954	a 3D printing technique	1932:1954	a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing	1932:2061	In addition, the present invention can be applied to a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing.
30506688	5	37	with	gum	1012:1014	arg1	capacity					1049:1056	its higher water hydration capacity	1022:1056	its higher water hydration capacity	1022:1056	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	0	38	theme	Hydrocolloids	10:22	arg1	Effect					0:5	Effect	0:5	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.	0:105	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	1	39	theme	main	251:254	arg1	source					194:199	the source	190:199	the source of the particles	190:216	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	39	theme	main	251:254	arg1	factors					256:262	the main factors	247:262	the main factors affecting the printability and rheological properties of the system	247:330	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	39	theme	main	251:254	arg1	content					235:241	the particle content	222:241	the particle content	222:241	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	4	40	theme	greatest	910:917	arg1	differences					919:929	the greatest differences	906:929	the greatest differences in rheology and printability	906:958	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	0	41	theme	Rheological	27:37	arg1	Properties					39:48	Rheological Properties	27:48	Rheological Properties	27:48	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	7	42	theme	xanthan	1460:1466	arg1	gum					1468:1470	xanthan gum	1460:1470	xanthan gum	1460:1470	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	2	43	theme	vegetable	388:396	arg1	powders					429:435	vegetable (broccoli, spinach, or carrot) powders	388:435	vegetable (broccoli, spinach, or carrot) powders	388:435	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	2	44	theme	powders	429:435	arg1	contents					358:365	different contents	348:365	different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders	348:435	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	6	45	theme	inks	1333:1336	arg1	analysis					1307:1314	Confocal laser scanning microscopy analysis	1272:1314	Confocal laser scanning microscopy analysis of the vegetable inks	1272:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	1	46	theme	rheological	295:305	arg1	properties					307:316	rheological properties	295:316	rheological properties	295:316	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	3	47	theme	powder	602:607	arg1	contents					609:616	low powder contents	598:616	low powder contents (10%)	598:622	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	3	47	theme	powder	602:607	arg1	%					621:621	10%	619:621	10%	619:621	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	6	48	theme	powder	1426:1431	arg1	source					1433:1438	the vegetable powder source	1412:1438	the vegetable powder source	1412:1438	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	9	49	theme	hydrocolloid	1980:1991	arg1	matrix					1993:1998	a hydrocolloid matrix	1978:1998	a hydrocolloid matrix	1978:1998	In addition, the present invention can be applied to a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing.
30506688	2	50	dep	contents	358:365	arg1	w/w					380:382	w/w	380:382	w/w	380:382	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	2	50	dep	contents	358:365	arg1	%					370:370	10% and 30% w/w	368:382	%	370:370	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	8	51	dep	APPLICATION	1670:1680	arg1	provides					1750:1757	provides	1750:1757	provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered	1750:1876	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	5	52	theme	hydration	1039:1047	arg1	capacity					1049:1056	its higher water hydration capacity	1022:1056	its higher water hydration capacity	1022:1056	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	8	53	theme	food	1794:1797	arg1	ink					1799:1801	food ink	1794:1801	food ink that can be universally applied to all food materials that can be powdered	1794:1876	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	1	54	theme	particle	226:233	arg1	source					194:199	the source	190:199	the source of the particles	190:216	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	54	theme	particle	226:233	arg1	factors					256:262	the main factors	247:262	the main factors affecting the printability and rheological properties of the system	247:330	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	54	theme	particle	226:233	arg1	content					235:241	the particle content	222:241	the particle content	222:241	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	5	55	from	increase	1120:1127	arg1	fractions					1170:1178	high volume fractions	1158:1178	high volume fractions of powder	1158:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	5	55	from	increase	1120:1127	arg1	values					1148:1153	the rheological values	1132:1153	the rheological values at high volume fractions of powder	1132:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	1	56	theme	hydrocolloid	169:180	arg1	matrix					182:187	a hydrocolloid matrix	167:187	a hydrocolloid matrix	167:187	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	2	57	dep	printability	537:548	arg1	the					533:535	the	533:535	the	533:535	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	7	58	theme	2.96	1551:1554	arg1	±					1556:1556	±	1556:1556	±	1556:1556	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	2	59	theme	hydrocolloid	451:462	arg1	matrices					464:471	hydrocolloid matrices	451:471	hydrocolloid matrices with different hydration properties	451:507	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	6	60	theme	microscopy	1296:1305	arg1	analysis					1307:1314	Confocal laser scanning microscopy analysis	1272:1314	Confocal laser scanning microscopy analysis of the vegetable inks	1272:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	7	61	theme	low	1528:1530	arg1	hardness					1541:1548	their low extruded hardness	1522:1548	their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg)	1522:1580	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	7	61	theme	low	1528:1530	arg1	kg					1578:1579	2.96 ± 0.23 to 3.46 ± 0.16 kg	1551:1579	2.96 ± 0.23 to 3.46 ± 0.16 kg	1551:1579	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	3	62	located	observed	692:699	arg2	differences					632:642	slight differences	625:642	slight differences in the printability and rheological values	625:685	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	3	62	located	observed	692:699	arg1	contents					609:616	low powder contents	598:616	low powder contents (10%)	598:622	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	3	62	located	observed	692:699	arg1	%					621:621	10%	619:621	10%	619:621	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	5	63	theme	powder	1183:1188	arg1	fractions					1170:1178	high volume fractions	1158:1178	high volume fractions of powder	1158:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	8	64	theme	PRACTICAL	1660:1668	arg1	APPLICATION					1670:1680	PRACTICAL APPLICATION	1660:1680	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.	1660:1877	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	1	65	theme	ink	115:117	arg1	systems					119:125	food ink systems	110:125	food ink systems in which the particles are dispersed in a hydrocolloid matrix	110:187	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	10	66	theme	inherent	2096:2103	arg1	differences					2117:2127	the inherent rheological differences	2092:2127	the inherent rheological differences between formulations with different food sources and compositions	2092:2193	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	6	67	theme	vegetable	1323:1331	arg1	inks					1333:1336	the vegetable inks	1319:1336	the vegetable inks	1319:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	4	68	theme	hydroxypropyl	868:880	arg1	methylcellulose					882:896	hydroxypropyl methylcellulose	868:896	hydroxypropyl methylcellulose	868:896	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	4	68	theme	hydroxypropyl	868:880	arg1	hydrocolloid					813:824	the hydrocolloid	809:824	the hydrocolloid with the lowest water hydration capacity	809:865	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	7	69	dep	0.16	1573:1576	arg1	to					1563:1564	to	1563:1564	to	1563:1564	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	5	70	theme	high	1158:1161	arg1	fractions					1170:1178	high volume fractions	1158:1178	high volume fractions of powder	1158:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	6	71	theme	scanning	1287:1294	arg1	microscopy					1296:1305	Confocal laser scanning microscopy	1272:1305	Confocal laser scanning microscopy analysis of the vegetable inks	1272:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	10	72	with	formulations	2137:2148	arg1	compositions					2182:2193	compositions	2182:2193	compositions	2182:2193	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	10	72	with	formulations	2137:2148	arg1	sources					2170:2176	different food sources	2155:2176	different food sources	2155:2176	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	7	73	dep	±	1556:1556	arg1	0.16					1573:1576	0.16	1573:1576	0.16	1573:1576	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	2	74	dep	vegetable	388:396	arg1	spinach					409:415	spinach	409:415	spinach	409:415	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	2	74	dep	vegetable	388:396	arg1	carrot					421:426	carrot	421:426	carrot	421:426	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	2	74	dep	vegetable	388:396	arg1	broccoli					399:406	broccoli	399:406	broccoli	399:406	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	8	75	theme	powder-based	1687:1698	arg1	technology					1714:1723	The powder-based texturization technology	1683:1723	The powder-based texturization technology introduced in this study	1683:1748	PRACTICAL APPLICATION: The powder-based texturization technology introduced in this study provides a standardized method of preparing food ink that can be universally applied to all food materials that can be powdered.
30506688	7	76	theme	high	1616:1619	arg1	resolution					1621:1630	high resolution	1616:1630	high resolution	1616:1630	The mixtures using xanthan gum could be smoothly extruded from the nozzle due to their low extruded hardness (2.96 ± 0.23 to 3.46 ± 0.16 kg), and the resulting objects showed high resolution without collapse over time.
30506688	0	77	theme	Food	92:95	arg1	Printing					97:104	3D Food Printing	89:104	3D Food Printing	89:104	Effect of Hydrocolloids on Rheological Properties and Printability of Vegetable Inks for 3D Food Printing.
30506688	4	78	theme	water	842:846	arg1	capacity					858:865	the lowest water hydration capacity	831:865	the lowest water hydration capacity	831:865	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	1	79	dep	printability	278:289	arg1	the					274:276	the	274:276	the	274:276	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	5	80	theme	different	1243:1251	arg1	sources					1263:1269	different vegetable sources	1243:1269	different vegetable sources	1243:1269	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	6	81	theme	laser	1281:1285	arg1	microscopy					1296:1305	Confocal laser scanning microscopy	1272:1305	Confocal laser scanning microscopy analysis of the vegetable inks	1272:1336	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	4	82	dep	different	965:973	arg1	vegetable					975:983	vegetable	975:983	vegetable	975:983	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	4	83	used	used	998:1001	arg2	sources					985:991	different vegetable sources	965:991	different vegetable sources	965:991	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	2	84	with	matrices	464:471	arg1	properties					498:507	different hydration properties	478:507	different hydration properties	478:507	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	5	85	dep	inhibited	1059:1067	arg1	minimizing					1105:1114	minimizing	1105:1114	minimizing the increase in the rheological values at high volume fractions of powder	1105:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	5	85	dep	inhibited	1059:1067	arg1	reducing					1194:1201	reducing	1194:1201	reducing the differences in printability between different vegetable sources	1194:1269	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	2	86	theme	different	348:356	arg1	contents					358:365	different contents	348:365	different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders	348:435	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	5	87	theme	higher	1026:1031	arg1	capacity					1049:1056	its higher water hydration capacity	1022:1056	its higher water hydration capacity	1022:1056	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	4	88	with	hydrocolloid	813:824	arg1	capacity					858:865	the lowest water hydration capacity	831:865	the lowest water hydration capacity	831:865	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	9	89	theme	3D	1934:1935	arg1	technique					1946:1954	a 3D printing technique	1932:1954	a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing	1932:2061	In addition, the present invention can be applied to a 3D printing technique in which a powder and a hydrocolloid matrix are independently stored and mixed immediately before printing.
30506688	5	90	theme	particles	1089:1097	arg1	swelling					1073:1080	the swelling	1069:1080	the swelling of the particles	1069:1097	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	10	91	theme	rheological	2105:2115	arg1	differences					2117:2127	the inherent rheological differences	2092:2127	the inherent rheological differences between formulations with different food sources and compositions	2092:2193	This technique can minimize the inherent rheological differences between formulations with different food sources and compositions.
30506688	2	92	from	influence	520:528	arg1	printability					537:548	printability	537:548	printability	537:548	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	2	92	from	influence	520:528	arg1	properties					566:575	rheological properties	554:575	rheological properties	554:575	In this study, different contents (10% and 30% w/w) of vegetable (broccoli, spinach, or carrot) powders were added to hydrocolloid matrices with different hydration properties, and their influence on the printability and rheological properties was investigated.
30506688	6	93	theme	xanthan	1350:1356	arg1	gum					1358:1360	xanthan gum	1350:1360	xanthan gum	1350:1360	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	4	94	from	differences	919:929	arg1	printability					947:958	printability	947:958	printability	947:958	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	4	94	from	differences	919:929	arg1	rheology					934:941	rheology	934:941	rheology	934:941	When the powder content was increased to 30%, the hydrocolloid with the lowest water hydration capacity, hydroxypropyl methylcellulose, showed the greatest differences in rheology and printability when different vegetable sources were used.
30506688	3	95	theme	low	598:600	arg1	contents					609:616	low powder contents	598:616	low powder contents (10%)	598:622	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	3	95	theme	low	598:600	arg1	%					621:621	10%	619:621	10%	619:621	At low powder contents (10%), slight differences in the printability and rheological values were observed between the different vegetable sources in all hydrocolloids.
30506688	5	96	theme	Xanthan	1004:1010	arg1	gum					1012:1014	Xanthan gum	1004:1014	Xanthan gum	1004:1014	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	6	97	theme	vegetable	1416:1424	arg1	source					1433:1438	the vegetable powder source	1412:1438	the vegetable powder source	1412:1438	Confocal laser scanning microscopy analysis of the vegetable inks showed that xanthan gum inhibited swelling of the particles regardless of the vegetable powder source.
30506688	5	98	theme	rheological	1136:1146	arg1	values					1148:1153	the rheological values	1132:1153	the rheological values at high volume fractions of powder	1132:1188	Xanthan gum, with its higher water hydration capacity, inhibited the swelling of the particles, thus minimizing the increase in the rheological values at high volume fractions of powder and reducing the differences in printability between different vegetable sources.
30506688	1	99	theme	particles	208:216	arg1	source					194:199	the source	190:199	the source of the particles	190:216	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	99	theme	particles	208:216	arg1	factors					256:262	the main factors	247:262	the main factors affecting the printability and rheological properties of the system	247:330	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
30506688	1	99	theme	particles	208:216	arg1	content					235:241	the particle content	222:241	the particle content	222:241	In food ink systems in which the particles are dispersed in a hydrocolloid matrix, the source of the particles and the particle content are the main factors affecting the printability and rheological properties of the system.
31386201	3	0	dep	US1	303:305	arg1	W L-1					312:316	542 W L-1	308:316	542 W L-1	308:316	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	3	0	dep	US1	303:305	arg1	794 W L-1					327:335	794 W L-1	327:335	US1: 542 W L-1 and US2: 794 W L-1	303:335	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	3	0	dep	US1	303:305	arg1	US2					322:324	US2	322:324	US2	322:324	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	4	1	theme	citric	466:471	arg1	acid					473:476	citric acid	466:476	citric acid	466:476	All extractions were carried out at 25 °C, using citric acid as the extraction solvent.
31386201	4	1	theme	citric	466:471	arg1	solvent					496:502	the extraction solvent	481:502	the extraction solvent	481:502	All extractions were carried out at 25 °C, using citric acid as the extraction solvent.
31386201	3	2	theme	mechanical	258:267	arg1	0.2 × g					280:286	0.2 × g	280:286	0.2 × g	280:286	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	3	2	theme	mechanical	258:267	arg1	agitation					269:277	mechanical agitation	258:277	mechanical agitation (0.2 × g)	258:287	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	5	3	theme	RESULTS	505:511	arg1	yields					554:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	6	4	dep	pH.	745:747	arg1	increased					779:787	increased	779:787	increased	779:787	Moreover, yield increases were significantly higher using the more acidic pH. Thus, at pH 1.5, pectin yield increased from ∼19%, obtained with agitation, to ∼47%, applying ultrasound; whereas, at pH 2.0, this increase was from ∼10%, with agitation, to ∼18%, applying ultrasound.
31386201	9	5	theme	relative	1328:1335	arg1	model					1308:1312	a second-order rate model	1288:1312	a second-order rate model (average mean relative error ≤ 7.4%)	1288:1349	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	9	5	theme	relative	1328:1335	arg1	%					1348:1348	average mean relative error ≤ 7.4%	1315:1348	average mean relative error ≤ 7.4%	1315:1348	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	0	6	theme	citrus	75:80	arg1	by-products					82:92	citrus by-products	75:92	citrus by-products	75:92	Effects of acoustic power and pH on pectin-enriched extracts obtained from citrus by-products.
31386201	3	7	theme	agitation	269:277	arg1	Kinetics					246:253	Kinetics	246:253	Kinetics of mechanical agitation (0.2 × g)	246:287	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	3	7	theme	agitation	269:277	arg1	extractions					339:349	acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions	293:349	acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions	293:349	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	5	8	theme	ultrasonic	580:589	arg1	assistance					591:600	ultrasonic assistance	580:600	ultrasonic assistance	580:600	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	10	9	theme	rate	1367:1370	arg1	constant					1372:1379	The extraction rate constant	1352:1379	The extraction rate constant	1352:1379	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	11	10	from	efficiency	1663:1672	arg1	process					1713:1719	the pectin extraction process	1691:1719	the pectin extraction process	1691:1719	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	5	11	theme	mechanical	649:658	arg1	agitation					660:668	mechanical agitation	649:668	mechanical agitation	649:668	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	11	12	theme	ultrasound	1677:1686	arg1	efficiency					1663:1672	the efficiency	1659:1672	the efficiency of ultrasound on the pectin extraction process	1659:1719	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	2	13	from	by-products	216:226	arg1	extraction					167:176	The ultrasound-assisted extraction	143:176	The ultrasound-assisted extraction of pectic polysaccharides from orange by-products	143:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	2	13	from	by-products	216:226	arg1	polysaccharides					188:202	pectic polysaccharides	181:202	pectic polysaccharides from orange by-products	181:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	9	14	theme	acoustic	1213:1220	arg1	extractions					1247:1257	acoustic and mechanical agitation extractions	1213:1257	acoustic and mechanical agitation extractions	1213:1257	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	11	15	theme	adequate	1596:1603	arg1	conditions					1612:1621	adequate acidic conditions	1596:1621	adequate acidic conditions	1596:1621	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	3	16	dep	values	395:400	arg1	2.0					411:413	2.0	411:413	2.0	411:413	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	3	16	dep	values	395:400	arg1	1.5					403:405	1.5	403:405	1.5	403:405	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	9	17	theme	mechanical	1226:1235	arg1	extractions					1247:1257	acoustic and mechanical agitation extractions	1213:1257	acoustic and mechanical agitation extractions	1213:1257	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	2	18	theme	ultrasound-assisted	147:165	arg1	extraction					167:176	The ultrasound-assisted extraction	143:176	The ultrasound-assisted extraction of pectic polysaccharides from orange by-products	143:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	10	19	theme	initial	1382:1388	arg1	rate					1401:1404	initial extraction rate	1382:1404	initial extraction rate	1382:1404	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	6	20	with	%	901:901	arg1	agitation					909:917	agitation	909:917	agitation	909:917	Moreover, yield increases were significantly higher using the more acidic pH. Thus, at pH 1.5, pectin yield increased from ∼19%, obtained with agitation, to ∼47%, applying ultrasound; whereas, at pH 2.0, this increase was from ∼10%, with agitation, to ∼18%, applying ultrasound.
31386201	11	21	theme	pectin	1695:1700	arg1	process					1713:1719	the pectin extraction process	1691:1719	the pectin extraction process	1691:1719	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	9	22	theme	second-order	1290:1301	arg1	model					1308:1312	a second-order rate model	1288:1312	a second-order rate model (average mean relative error ≤ 7.4%)	1288:1349	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	9	22	theme	second-order	1290:1301	arg1	%					1348:1348	average mean relative error ≤ 7.4%	1315:1348	average mean relative error ≤ 7.4%	1315:1348	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	3	23	theme	different	382:390	arg1	values					395:400	different pH values	382:400	different pH values (1.5 and 2.0)	382:414	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	9	24	theme	agitation	1237:1245	arg1	extractions					1247:1257	acoustic and mechanical agitation extractions	1213:1257	acoustic and mechanical agitation extractions	1213:1257	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	7	25	theme	acid	994:997	arg1	proportion					999:1008	the galacturonic acid proportion	977:1008	the galacturonic acid proportion	977:1008	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	6	26	theme	pectin	766:771	arg1	yield					773:777	pectin yield	766:777	pectin yield	766:777	Moreover, yield increases were significantly higher using the more acidic pH. Thus, at pH 1.5, pectin yield increased from ∼19%, obtained with agitation, to ∼47%, applying ultrasound; whereas, at pH 2.0, this increase was from ∼10%, with agitation, to ∼18%, applying ultrasound.
31386201	3	27	theme	pH	392:393	arg1	values					395:400	different pH values	382:400	different pH values (1.5 and 2.0)	382:414	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	9	28	theme	extractions	1247:1257	arg1	Curves					1203:1208	Curves	1203:1208	Curves of acoustic and mechanical agitation extractions	1203:1257	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	7	29	theme	proportion	999:1008	arg1	decrease					965:972	A considerable decrease	950:972	A considerable decrease of the galacturonic acid proportion	950:1008	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	10	30	theme	extraction	1390:1399	arg1	rate					1401:1404	initial extraction rate	1382:1404	initial extraction rate	1382:1404	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	6	31	theme	yield	681:685	arg1	increases					687:695	yield increases	681:695	yield increases	681:695	Moreover, yield increases were significantly higher using the more acidic pH. Thus, at pH 1.5, pectin yield increased from ∼19%, obtained with agitation, to ∼47%, applying ultrasound; whereas, at pH 2.0, this increase was from ∼10%, with agitation, to ∼18%, applying ultrasound.
31386201	10	32	theme	extraction	1356:1365	arg1	constant					1372:1379	The extraction rate constant	1352:1379	The extraction rate constant	1352:1379	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	0	33	theme	power	20:24	arg1	Effects					0:6	Effects	0:6	Effects of acoustic power and pH	0:31	Effects of acoustic power and pH on pectin-enriched extracts obtained from citrus by-products.
31386201	12	34	theme	Chemical	1740:1747	arg1	Industry					1749:1756	Chemical Industry	1740:1756	Chemical Industry	1740:1756	© 2019 Society of Chemical Industry.
31386201	0	35	theme	acoustic	11:18	arg1	power					20:24	acoustic power	11:24	acoustic power	11:24	Effects of acoustic power and pH on pectin-enriched extracts obtained from citrus by-products.
31386201	11	36	theme	extraction	1702:1711	arg1	process					1713:1719	the pectin extraction process	1691:1719	the pectin extraction process	1691:1719	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	7	37	theme	galacturonic	981:992	arg1	proportion					999:1008	the galacturonic acid proportion	977:1008	the galacturonic acid proportion	977:1008	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	2	38	theme	orange	209:214	arg1	by-products					216:226	orange by-products	209:226	orange by-products	209:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	0	39	theme	pH	30:31	arg1	Effects					0:6	Effects	0:6	Effects of acoustic power and pH	0:31	Effects of acoustic power and pH on pectin-enriched extracts obtained from citrus by-products.
31386201	3	40	theme	acoustic	293:300	arg1	extractions					339:349	acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions	293:349	acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions	293:349	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	8	41	theme	methylation	1183:1193	arg1	degree					1195:1200	a low methylation degree	1177:1200	a low methylation degree	1177:1200	High methoxyl pectins were extracted at pH 1.5 whereas at pH 2.0, pectins exhibited a low methylation degree.
31386201	3	42	dep	extractions	339:349	arg1	US1					303:305	US1	303:305	US1: 542 W L-1 and US2: 794 W L-1	303:335	Kinetics of mechanical agitation (0.2 × g) and acoustic (US1: 542 W L-1 and US2: 794 W L-1 ) extractions, were obtained and modelled at different pH values (1.5 and 2.0).
31386201	2	43	theme	polysaccharides	188:202	arg1	extraction					167:176	The ultrasound-assisted extraction	143:176	The ultrasound-assisted extraction of pectic polysaccharides from orange by-products	143:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	5	44	theme	Higher	513:518	arg1	yields					554:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	8	45	theme	low	1179:1181	arg1	degree					1195:1200	a low methylation degree	1177:1200	a low methylation degree	1177:1200	High methoxyl pectins were extracted at pH 1.5 whereas at pH 2.0, pectins exhibited a low methylation degree.
31386201	4	46	theme	extraction	485:494	arg1	acid					473:476	citric acid	466:476	citric acid	466:476	All extractions were carried out at 25 °C, using citric acid as the extraction solvent.
31386201	4	46	theme	extraction	485:494	arg1	solvent					496:502	the extraction solvent	481:502	the extraction solvent	481:502	All extractions were carried out at 25 °C, using citric acid as the extraction solvent.
31386201	2	47	theme	pectic	181:186	arg1	polysaccharides					188:202	pectic polysaccharides	181:202	pectic polysaccharides from orange by-products	181:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	5	48	theme	pectic	520:525	arg1	yields					554:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	1	49	theme	extraction	112:121	arg1	process					123:129	the extraction process	108:129	the extraction process	108:129	Modelling of the extraction process.
31386201	2	50	from	extraction	167:176	arg1	by-products					216:226	orange by-products	209:226	orange by-products	209:226	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	2	51	dep	BACKGROUND	132:141	arg1	investigated					232:243	investigated	232:243	was investigated	228:243	BACKGROUND The ultrasound-assisted extraction of pectic polysaccharides from orange by-products was investigated.
31386201	7	52	theme	considerable	952:963	arg1	decrease					965:972	A considerable decrease	950:972	A considerable decrease of the galacturonic acid proportion	950:1008	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	5	53	theme	polysaccharides	527:541	arg1	yields					554:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	1	54	theme	process	123:129	arg1	Modelling					95:103	Modelling	95:103	Modelling of the extraction process	95:129	Modelling of the extraction process.
31386201	7	55	located	observed	1014:1021	arg1	extracts					1030:1037	the extracts	1026:1037	the extracts	1026:1037	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	7	55	located	observed	1014:1021	arg2	decrease					965:972	A considerable decrease	950:972	A considerable decrease of the galacturonic acid proportion	950:1008	A considerable decrease of the galacturonic acid proportion was observed on the extracts when ultrasound were applied for 60 min under pH 2.0.
31386201	9	56	theme	rate	1303:1306	arg1	model					1308:1312	a second-order rate model	1288:1312	a second-order rate model (average mean relative error ≤ 7.4%)	1288:1349	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	9	56	theme	rate	1303:1306	arg1	%					1348:1348	average mean relative error ≤ 7.4%	1315:1348	average mean relative error ≤ 7.4%	1315:1348	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	11	57	theme	acidic	1605:1610	arg1	conditions					1612:1621	adequate acidic conditions	1596:1621	adequate acidic conditions	1596:1621	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	5	58	theme	extraction	543:552	arg1	yields					554:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields	505:559	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	5	59	with	comparison	606:615	arg1	results					626:632	the results	622:632	the results obtained using mechanical agitation	622:668	RESULTS Higher pectic polysaccharides extraction yields were obtained with ultrasonic assistance, in comparison with the results obtained using mechanical agitation.
31386201	8	60	theme	High	1093:1096	arg1	pectins					1107:1113	High methoxyl pectins	1093:1113	High methoxyl pectins	1093:1113	High methoxyl pectins were extracted at pH 1.5 whereas at pH 2.0, pectins exhibited a low methylation degree.
31386201	11	61	theme	ultrasound	1542:1551	arg1	dependent					1564:1572	dependent	1564:1572	dependent	1564:1572	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	11	61	theme	ultrasound	1542:1551	arg1	effect					1532:1537	the effect	1528:1537	the effect of ultrasound	1528:1551	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	10	62	theme	maximum	1410:1416	arg1	yield					1418:1422	maximum yield	1410:1422	maximum yield	1410:1422	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	10	63	theme	experimental	1448:1459	arg1	conditions					1461:1470	all experimental conditions	1444:1470	all experimental conditions	1444:1470	The extraction rate constant, initial extraction rate and maximum yield were determined for all experimental conditions.
31386201	11	64	dep	CONCLUSION	1473:1482	arg1	indicated					1513:1521	indicated	1513:1521	indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process	1513:1719	CONCLUSION Overall, the results clearly indicated that the effect of ultrasound was highly dependent on the pH. Therefore, adequate acidic conditions must be applied in order to improve the efficiency of ultrasound on the pectin extraction process.
31386201	9	65	theme	average	1315:1321	arg1	model					1308:1312	a second-order rate model	1288:1312	a second-order rate model (average mean relative error ≤ 7.4%)	1288:1349	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	9	65	theme	average	1315:1321	arg1	%					1348:1348	average mean relative error ≤ 7.4%	1315:1348	average mean relative error ≤ 7.4%	1315:1348	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	8	66	theme	methoxyl	1098:1105	arg1	pectins					1107:1113	High methoxyl pectins	1093:1113	High methoxyl pectins	1093:1113	High methoxyl pectins were extracted at pH 1.5 whereas at pH 2.0, pectins exhibited a low methylation degree.
31386201	6	67	from	pH 2.0	867:872	arg1	%					901:901	∼10%	898:901	∼10%	898:901	Moreover, yield increases were significantly higher using the more acidic pH. Thus, at pH 1.5, pectin yield increased from ∼19%, obtained with agitation, to ∼47%, applying ultrasound; whereas, at pH 2.0, this increase was from ∼10%, with agitation, to ∼18%, applying ultrasound.
31386201	9	68	theme	mean	1323:1326	arg1	model					1308:1312	a second-order rate model	1288:1312	a second-order rate model (average mean relative error ≤ 7.4%)	1288:1349	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
31386201	9	68	theme	mean	1323:1326	arg1	%					1348:1348	average mean relative error ≤ 7.4%	1315:1348	average mean relative error ≤ 7.4%	1315:1348	Curves of acoustic and mechanical agitation extractions were properly represented by a second-order rate model (average mean relative error ≤ 7.4%).
29441088	4	0	theme	cholera	987:993	arg1	toxin					995:999	N-glycosylated cholera toxin	972:999	N-glycosylated cholera toxin B subunit (gCTB)	972:1016	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	1	theme	binding	596:602	arg1	affinity					604:611	high binding affinity	591:611	high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	591:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	8	2	theme	antigens	1863:1870	arg1	production					1809:1818	efficient production	1799:1818	efficient production of highly mannosylated recombinant vaccine antigens in plants	1799:1880	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	4	3	theme	B	1001:1001	arg1	gCTB					1012:1015	gCTB	1012:1015	gCTB	1012:1015	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	3	theme	B	1001:1001	arg1	subunit					1003:1009	N-glycosylated cholera toxin B subunit	972:1009	N-glycosylated cholera toxin B subunit (gCTB)	972:1016	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	1	4	theme	high	337:340	arg1	scalability					342:352	high scalability	337:352	high scalability	337:352	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	4	5	theme	glycoproteins	951:963	arg1	production					923:932	the production	919:932	the production of Man9-rich HMG glycoproteins	919:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	6	6	theme	Detailed	1400:1407	arg1	profiling					1416:1424	Detailed glycan profiling	1400:1424	Detailed glycan profiling	1400:1424	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	2	7	theme	vaccines	543:550	arg1	effectiveness					513:525	the effectiveness	509:525	the effectiveness of some subunit vaccines	509:550	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	1	8	theme	transient	216:224	arg1	overexpression					226:239	transient overexpression	216:239	Nicotiana benthamiana transient overexpression systems	194:247	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	3	9	theme	mannose	669:675	arg1	DC-SIGN					771:777	DC-SIGN	771:777	DC-SIGN	771:777	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	9	theme	mannose	669:675	arg1	receptor					677:684	mannose receptor	669:684	mannose receptor	669:684	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	4	10	theme	human	1022:1026	arg1	virus					1045:1049	human immunodeficiency virus	1022:1049	human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1022:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	0	11	theme	Man9	140:143	arg1	Type					158:161	Predominantly Man9 High-Mannose Type	126:161	Predominantly Man9 High-Mannose Type	126:161	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	4	12	gly	glycoproteins	951:963	arg1	glycoproteins					951:963	Man9-rich HMG glycoproteins	937:963	Man9-rich HMG glycoproteins	937:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	13	theme	cell-specific	700:712	arg1	molecule					737:744	dendritic cell-specific intracellular adhesion molecule	690:744	dendritic cell-specific intracellular adhesion molecule	690:744	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	0	14	theme	Transient	168:176	arg1	Overexpression					178:191	Transient Overexpression	168:191	Transient Overexpression	168:191	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	5	15	theme	Biochemical	1153:1163	arg1	analysis					1165:1172	Biochemical analysis	1153:1172	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion	1153:1258	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	16	theme	α-mannosidase	833:845	arg1	inhibitor					849:857	an α-mannosidase I inhibitor	830:857	an α-mannosidase I inhibitor	830:857	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	16	theme	α-mannosidase	833:845	arg1	kifunensine					817:827	kifunensine	817:827	kifunensine	817:827	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	17	theme	adhesion	728:735	arg1	molecule					737:744	dendritic cell-specific intracellular adhesion molecule	690:744	dendritic cell-specific intracellular adhesion molecule	690:744	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	4	18	theme	endoplasmic	1087:1097	arg1	reticulum					1099:1107	a H/KDEL endoplasmic reticulum	1078:1107	a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1078:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	1	19	theme	eukaryotic	358:367	arg1	modifications					388:400	eukaryotic post-translational modifications	358:400	eukaryotic post-translational modifications such as N-glycosylation	358:424	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	1	19	theme	eukaryotic	358:367	arg1	N-glycosylation					410:424	N-glycosylation	410:424	N-glycosylation	410:424	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	5	20	theme	F	1248:1248	arg1	digestion					1250:1258	Endo H and PNGase F digestion	1230:1258	digestion	1250:1258	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	7	21	theme	Man9	1735:1738	arg1	composition					1740:1750	a substantially lower Man9 composition	1713:1750	a substantially lower Man9 composition	1713:1750	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	0	22	theme	Glycoprotein	101:112	arg1	Antigens					114:121	Recombinant Glycoprotein Antigens	89:121	Recombinant Glycoprotein Antigens	89:121	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	8	23	from	production	1809:1818	arg1	plants					1875:1880	plants	1875:1880	plants	1875:1880	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	5	24	theme	Endo	1230:1233	arg1	H					1235:1235	Endo H and PNGase F digestion	1230:1258	H	1235:1235	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	25	theme	vaccine	1135:1141	arg1	antigens					1143:1150	model vaccine antigens	1129:1150	model vaccine antigens	1129:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	0	26	theme	Hydroponic	0:9	arg1	Treatment					11:19	Hydroponic Treatment	0:19	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine	0:61	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	8	27	gly	mannosylated	1830:1841	arg1	antigens					1863:1870	highly mannosylated recombinant vaccine antigens	1823:1870	highly mannosylated recombinant vaccine antigens	1823:1870	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	2	28	theme	subunit	535:541	arg1	vaccines					543:550	some subunit vaccines	530:550	some subunit vaccines	530:550	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	5	29	theme	plant-specific	1314:1327	arg1	glycoforms					1329:1338	plant-specific glycoforms	1314:1338	plant-specific glycoforms	1314:1338	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	30	theme	retention	1109:1117	arg1	signal					1119:1124	a H/KDEL endoplasmic reticulum retention signal	1078:1124	a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1078:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	31	dep	receptor	677:684	arg1	the					665:667	the	665:667	the	665:667	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	0	32	theme	benthamiana	34:44	arg1	Treatment					11:19	Hydroponic Treatment	0:19	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine	0:61	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	4	33	theme	Man9-rich	937:945	arg1	glycoproteins					951:963	Man9-rich HMG glycoproteins	937:963	Man9-rich HMG glycoproteins	937:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	5	34	theme	kifunensine	1272:1282	arg1	treatment					1284:1292	kifunensine treatment	1272:1292	kifunensine treatment	1272:1292	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	35	theme	N.	900:901	arg1	benthamiana					903:913	N. benthamiana	900:913	N. benthamiana	900:913	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	2	36	theme	High-mannose-type	427:443	arg1	glycans					445:451	High-mannose-type glycans	427:451	High-mannose-type glycans (HMGs) of glycoprotein antigens	427:483	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	2	36	theme	High-mannose-type	427:443	arg1	HMGs					454:457	HMGs	454:457	HMGs	454:457	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	8	37	theme	efficient	1799:1807	arg1	production					1809:1818	efficient production	1799:1818	efficient production of highly mannosylated recombinant vaccine antigens in plants	1799:1880	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	1	38	theme	rapid	277:281	arg1	production					315:324	rapid and scalable biopharmaceuticals production	277:324	rapid and scalable biopharmaceuticals production	277:324	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	3	39	theme	C-type	633:638	arg1	lectin					640:645	mannose-specific C-type lectin	616:645	mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	616:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	2	40	theme	glycoprotein	463:474	arg1	antigens					476:483	glycoprotein antigens	463:483	glycoprotein antigens	463:483	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	1	41	theme	scalable	287:294	arg1	production					315:324	rapid and scalable biopharmaceuticals production	277:324	rapid and scalable biopharmaceuticals production	277:324	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	6	42	theme	plant-produced	1440:1453	arg1	gp120					1455:1459	plant-produced gp120	1440:1459	plant-produced gp120	1440:1459	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	3	43	theme	high	591:594	arg1	affinity					604:611	high binding affinity	591:611	high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	591:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	8	44	theme	mannosylated	1830:1841	arg1	antigens					1863:1870	highly mannosylated recombinant vaccine antigens	1823:1870	highly mannosylated recombinant vaccine antigens	1823:1870	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	1	45	theme	biopharmaceuticals	296:313	arg1	production					315:324	rapid and scalable biopharmaceuticals production	277:324	rapid and scalable biopharmaceuticals production	277:324	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	4	46	theme	N-glycosylated	972:985	arg1	toxin					995:999	N-glycosylated cholera toxin	972:999	N-glycosylated cholera toxin B subunit (gCTB)	972:1016	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	47	theme	lectin	640:645	arg1	receptor					677:684	mannose receptor	669:684	mannose receptor	669:684	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	47	theme	lectin	640:645	arg1	receptors					647:655	mannose-specific C-type lectin receptors	616:655	mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	616:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	47	theme	lectin	640:645	arg1	molecule					737:744	dendritic cell-specific intracellular adhesion molecule	690:744	dendritic cell-specific intracellular adhesion molecule	690:744	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	8	48	theme	vaccine	1855:1861	arg1	antigens					1863:1870	highly mannosylated recombinant vaccine antigens	1823:1870	highly mannosylated recombinant vaccine antigens	1823:1870	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	7	49	theme	prominent	1633:1641	arg1	glycoform					1643:1651	the most prominent glycoform	1624:1651	the most prominent glycoform (64.5%)	1624:1659	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	7	49	theme	prominent	1633:1641	arg1	%					1658:1658	64.5%	1654:1658	64.5%	1654:1658	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	4	50	theme	toxin	995:999	arg1	gCTB					1012:1015	gCTB	1012:1015	gCTB	1012:1015	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	50	theme	toxin	995:999	arg1	subunit					1003:1009	N-glycosylated cholera toxin B subunit	972:1009	N-glycosylated cholera toxin B subunit (gCTB)	972:1016	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	6	51	theme	glycan	1409:1414	arg1	profiling					1416:1424	Detailed glycan profiling	1400:1424	Detailed glycan profiling	1400:1424	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	3	52	theme	mannose-specific	616:631	arg1	lectin					640:645	mannose-specific C-type lectin	616:645	mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	616:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	4	53	gly	N-glycosylated	972:985	arg1	toxin					995:999	N-glycosylated cholera toxin	972:999	N-glycosylated cholera toxin B subunit (gCTB)	972:1016	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	54	theme	HMG	947:949	arg1	glycoproteins					951:963	Man9-rich HMG glycoproteins	937:963	Man9-rich HMG glycoproteins	937:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	1	55	theme	overexpression	226:239	arg1	systems					241:247	Nicotiana benthamiana transient overexpression systems	194:247	Nicotiana benthamiana transient overexpression systems	194:247	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	7	56	theme	kifunensine-treatment	1573:1593	arg1	conditions					1595:1604	kifunensine-treatment conditions	1573:1604	kifunensine-treatment conditions	1573:1604	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	0	57	theme	High-Mannose	145:156	arg1	Type					158:161	Predominantly Man9 High-Mannose Type	126:161	Predominantly Man9 High-Mannose Type	126:161	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	5	58	theme	anti-xylose	1196:1206	arg1	antibodies					1208:1217	anti-fucose and anti-xylose antibodies	1180:1217	anti-fucose and anti-xylose antibodies	1180:1217	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	3	59	theme	dendritic	690:698	arg1	molecule					737:744	dendritic cell-specific intracellular adhesion molecule	690:744	dendritic cell-specific intracellular adhesion molecule	690:744	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	7	60	contain	had	1709:1711	arg1	protein					1672:1678	the protein	1668:1678	the protein produced without kifunensine	1668:1707	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	7	60	contain	had	1709:1711	arg2	composition					1740:1750	a substantially lower Man9 composition	1713:1750	a substantially lower Man9 composition	1713:1750	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	4	61	theme	immunodeficiency	1028:1043	arg1	virus					1045:1049	human immunodeficiency virus	1022:1049	human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1022:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	62	dep	virus	1045:1049	arg1	gp120					1051:1055	gp120	1051:1055	human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1022:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	6	63	theme	glycan	1467:1472	arg1	profile					1474:1480	a glycan profile	1465:1480	a glycan profile bearing mostly HMGs	1465:1500	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	5	64	from	HMGs	1357:1360	arg1	compositions					1378:1389	the N-glycan compositions	1365:1389	the N-glycan compositions of gCTB	1365:1397	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	65	theme	kifunensine	817:827	arg1	effect					807:812	the effect	803:812	the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins	803:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	5	66	theme	anti-fucose	1180:1190	arg1	antibodies					1208:1217	anti-fucose and anti-xylose antibodies	1180:1217	anti-fucose and anti-xylose antibodies	1180:1217	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	3	67	theme	intracellular	714:726	arg1	molecule					737:744	dendritic cell-specific intracellular adhesion molecule	690:744	dendritic cell-specific intracellular adhesion molecule	690:744	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	5	68	theme	gCTB	1394:1397	arg1	compositions					1378:1389	the N-glycan compositions	1365:1389	the N-glycan compositions of gCTB	1365:1397	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	6	69	theme	kifunensine	1516:1526	arg1	treatment					1528:1536	kifunensine treatment	1516:1536	kifunensine treatment	1516:1536	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	0	70	theme	Recombinant	89:99	arg1	Antigens					114:121	Recombinant Glycoprotein Antigens	89:121	Recombinant Glycoprotein Antigens	89:121	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	5	71	theme	PNGase	1241:1246	arg1	digestion					1250:1258	Endo H and PNGase F digestion	1230:1258	digestion	1250:1258	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	6	72	contain	had	1461:1463	arg1	gp120					1455:1459	plant-produced gp120	1440:1459	plant-produced gp120	1440:1459	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	6	72	contain	had	1461:1463	arg2	profile					1474:1480	a glycan profile	1465:1480	a glycan profile bearing mostly HMGs	1465:1500	Detailed glycan profiling revealed that plant-produced gp120 had a glycan profile bearing mostly HMGs regardless of kifunensine treatment.
29441088	7	73	theme	lower	1729:1733	arg1	composition					1740:1750	a substantially lower Man9 composition	1713:1750	a substantially lower Man9 composition	1713:1750	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29441088	4	74	theme	H/KDEL	1080:1085	arg1	reticulum					1099:1107	a H/KDEL endoplasmic reticulum	1078:1107	a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1078:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	0	75	theme	Antigens	114:121	arg1	N-glycans					76:84	the N-glycans	72:84	the N-glycans of Recombinant Glycoprotein Antigens	72:121	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	1	76	theme	unique	255:260	arg1	scalability					342:352	high scalability	337:352	high scalability	337:352	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	1	76	theme	unique	255:260	arg1	advantages					262:271	unique advantages	255:271	unique advantages	255:271	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	1	76	theme	unique	255:260	arg1	modifications					388:400	eukaryotic post-translational modifications	358:400	eukaryotic post-translational modifications such as N-glycosylation	358:424	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	3	77	contain	has	587:589	arg2	affinity					604:611	high binding affinity	591:611	high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)	591:778	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	77	contain	has	587:589	arg1	Man9					581:584	Man9	581:584	Man9	581:584	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	77	contain	has	587:589	arg1	Man9GlcNAc2					568:578	Man9GlcNAc2	568:578	Man9GlcNAc2 (Man9)	568:585	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	4	78	theme	hydroponic	878:887	arg1	culture					889:895	a hydroponic culture	876:895	a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins	876:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	79	theme	model	1129:1133	arg1	antigens					1143:1150	model vaccine antigens	1129:1150	model vaccine antigens	1129:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	80	theme	I	847:847	arg1	inhibitor					849:857	an α-mannosidase I inhibitor	830:857	an α-mannosidase I inhibitor	830:857	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	4	80	theme	I	847:847	arg1	kifunensine					817:827	kifunensine	817:827	kifunensine	817:827	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	2	81	theme	antigens	476:483	arg1	glycans					445:451	High-mannose-type glycans	427:451	High-mannose-type glycans (HMGs) of glycoprotein antigens	427:483	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	2	81	theme	antigens	476:483	arg1	HMGs					454:457	HMGs	454:457	HMGs	454:457	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	0	82	theme	Nicotiana	24:32	arg1	benthamiana					34:44	Nicotiana benthamiana	24:44	Nicotiana benthamiana	24:44	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	2	83	gly	glycoprotein	463:474	arg1	glycoprotein					463:474	glycoprotein antigens	463:483	glycoprotein antigens	463:483	High-mannose-type glycans (HMGs) of glycoprotein antigens have been implicated in the effectiveness of some subunit vaccines.
29441088	1	84	theme	post-translational	369:386	arg1	modifications					388:400	eukaryotic post-translational modifications	358:400	eukaryotic post-translational modifications such as N-glycosylation	358:424	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	1	84	theme	post-translational	369:386	arg1	N-glycosylation					410:424	N-glycosylation	410:424	N-glycosylation	410:424	Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation.
29441088	4	85	theme	reticulum	1099:1107	arg1	signal					1119:1124	a H/KDEL endoplasmic reticulum retention signal	1078:1124	a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens	1078:1150	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	3	86	theme	non-integrin	757:768	arg1	DC-SIGN					771:777	DC-SIGN	771:777	DC-SIGN	771:777	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	3	86	theme	non-integrin	757:768	arg1	receptor					677:684	mannose receptor	669:684	mannose receptor	669:684	In particular, Man9GlcNAc2 (Man9) has high binding affinity to mannose-specific C-type lectin receptors such as the mannose receptor and dendritic cell-specific intracellular adhesion molecule 3-grabbing non-integrin (DC-SIGN).
29441088	0	87	with	Treatment	11:19	arg1	Kifunensine					51:61	Kifunensine	51:61	Kifunensine	51:61	Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression.
29441088	5	88	theme	N-glycan	1369:1376	arg1	compositions					1378:1389	the N-glycan compositions	1365:1389	the N-glycan compositions of gCTB	1365:1397	Biochemical analysis using anti-fucose and anti-xylose antibodies as well as Endo H and PNGase F digestion showed that kifunensine treatment effectively reduced plant-specific glycoforms while increasing HMGs in the N-glycan compositions of gCTB.
29441088	4	89	theme	benthamiana	903:913	arg1	culture					889:895	a hydroponic culture	876:895	a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins	876:963	Here, we investigated the effect of kifunensine, an α-mannosidase I inhibitor, supplemented in a hydroponic culture of N. benthamiana for the production of Man9-rich HMG glycoproteins, using N-glycosylated cholera toxin B subunit (gCTB) and human immunodeficiency virus gp120 that are tagged with a H/KDEL endoplasmic reticulum retention signal as model vaccine antigens.
29441088	8	90	theme	recombinant	1843:1853	arg1	antigens					1863:1870	highly mannosylated recombinant vaccine antigens	1823:1870	highly mannosylated recombinant vaccine antigens	1823:1870	Our results open up possibilities for efficient production of highly mannosylated recombinant vaccine antigens in plants.
29441088	7	91	dep	had	1709:1711	arg1	%					1757:1757	20.3%	1753:1757	20.3%	1753:1757	However, the gp120 produced under kifunensine-treatment conditions showed Man9 being the most prominent glycoform (64.5%), while the protein produced without kifunensine had a substantially lower Man9 composition (20.3%).
29302878	0	0	theme	Dietary	77:83	arg1	Restriction					92:102	Dietary FODMAP Restriction	77:102	Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	77:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	6	1	theme	taxonomic	904:912	arg1	levels					914:919	different taxonomic levels	894:919	different taxonomic levels	894:919	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	5	2	theme	as ≥ 50	650:656	arg1	%					657:657	%	657:657	%	657:657	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	10	3	dep	10	1431:1432	arg1	to					1428:1429	to	1428:1429	to	1428:1429	These points were summed up, giving an RI from 0 to 10.
29302878	5	4	theme	IBS	671:673	arg1	IBS-SSS					700:706	IBS-SSS	700:706	IBS-SSS	700:706	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	5	4	theme	IBS	671:673	arg1	scores					692:697	IBS symptom severity scores	671:697	IBS symptom severity scores (IBS-SSS)	671:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	9	5	theme	responders	1191:1200	arg1	values					1164:1169	median values	1157:1169	median values (used as cutoff) of responders for these 10 bacterial markers	1157:1231	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	6	6	theme	different	894:902	arg1	levels					914:919	different taxonomic levels	894:919	different taxonomic levels	894:919	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	5	7	theme	%	657:657	arg1	decrease					659:666	defined as ≥ 50% decrease	642:666	defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS)	642:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	6	8	theme	Dysbiosis	810:818	arg1	Test					820:823	the GA-map™ Dysbiosis Test	798:823	the GA-map™ Dysbiosis Test	798:823	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	6	8	theme	Dysbiosis	810:818	arg1	method					790:795	a commercially available method	765:795	a commercially available method (the GA-map™ Dysbiosis Test)	765:824	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	12	9	theme	dietary	1728:1734	arg1	restriction					1743:1753	dietary FODMAP restriction	1728:1753	dietary FODMAP restriction	1728:1753	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	11	10	theme	likely	1474:1479	arg1	times					1463:1467	5 times	1461:1467	5 times more likely	1461:1479	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	3	11	with	patients	505:512	arg1	IBS					519:521	IBS	519:521	IBS	519:521	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	6	12	theme	GA-map™	802:808	arg1	Test					820:823	the GA-map™ Dysbiosis Test	798:823	the GA-map™ Dysbiosis Test	798:823	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	6	12	theme	GA-map™	802:808	arg1	method					790:795	a commercially available method	765:795	a commercially available method (the GA-map™ Dysbiosis Test)	765:824	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	4	13	theme	4-week	590:595	arg1	diet					615:618	a 4-week FODMAP-restricted diet	588:618	a 4-week FODMAP-restricted diet	588:618	METHODS Patients were recruited consecutively to participate in a 4-week FODMAP-restricted diet.
29302878	0	14	theme	FODMAP	85:90	arg1	Restriction					92:102	Dietary FODMAP Restriction	77:102	Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	77:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	8	15	theme	markers	1078:1084	arg1	Ten					1054:1056	Ten	1054:1056	Ten	1054:1056	Ten of the 54 bacterial markers differed significantly between responders and non-responders.
29302878	8	15	theme	markers	1078:1084	arg1	markers					1078:1084	the 54 bacterial markers	1061:1084	the 54 bacterial markers	1061:1084	Ten of the 54 bacterial markers differed significantly between responders and non-responders.
29302878	9	16	theme	bacterial	1215:1223	arg1	markers					1225:1231	these 10 bacterial markers	1206:1231	these 10 bacterial markers	1206:1231	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	12	17	theme	new	1647:1649	arg1	RI					1651:1652	a new RI	1645:1652	a new RI	1645:1652	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	7	18	dep	RESULTS	922:928	arg1	included					966:973	included	966:973	were included	961:973	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	18	dep	RESULTS	922:928	arg1	classified					991:1000	classified	991:1000	classified as responders and 29 (25 F; 4 M) as non-responders	991:1051	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	1	19	theme	low	165:167	arg1	diet					160:163	A diet	158:163	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs)	147:254	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	8	20	theme	bacterial	1068:1076	arg1	markers					1078:1084	the 54 bacterial markers	1061:1084	the 54 bacterial markers	1061:1084	Ten of the 54 bacterial markers differed significantly between responders and non-responders.
29302878	7	21	theme	F	1027:1027	arg1	4 M					1030:1032	25 F; 4 M	1024:1032	25 F; 4 M	1024:1032	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	21	theme	F	1027:1027	arg1	non-responders					1038:1051	29 (25 F; 4 M) as non-responders	1020:1051	29 (25 F; 4 M) as non-responders	1020:1051	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	0	22	with	Patients	107:114	arg1	Syndrome					137:144	Irritable Bowel Syndrome	121:144	Irritable Bowel Syndrome	121:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	11	23	dep	probability	1535:1545	arg1	respond					1550:1556	respond	1550:1556	to respond	1547:1556	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	3	24	theme	microbial	429:437	arg1	composition					439:449	gut microbial composition	425:449	gut microbial composition	425:449	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	0	25	theme	Gut	10:12	arg1	Composition					25:35	Gut Microbiota Composition	10:35	Gut Microbiota Composition	10:35	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	0	25	theme	Gut	10:12	arg1	Indicator					43:51	an Indicator	40:51	an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	40:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	9	26	theme	Response	1251:1258	arg1	RI					1267:1268	RI	1267:1268	RI	1267:1268	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	9	26	theme	Response	1251:1258	arg1	Index					1260:1264	a Response Index	1249:1264	a Response Index (RI)	1249:1269	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	6	27	theme	available	780:788	arg1	Test					820:823	the GA-map™ Dysbiosis Test	798:823	the GA-map™ Dysbiosis Test	798:823	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	6	27	theme	available	780:788	arg1	method					790:795	a commercially available method	765:795	a commercially available method (the GA-map™ Dysbiosis Test)	765:824	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	1	28	theme	fermentable	172:182	arg1	oligosaccharides					184:199	fermentable oligosaccharides	172:199	fermentable oligosaccharides	172:199	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	28	theme	fermentable	172:182	arg1	FODMAPs					247:253	FODMAPs	247:253	FODMAPs	247:253	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	11	29	theme	%	1571:1571	arg1	%					1584:1584	95% CI [61.2-94%	1569:1584	95% CI [61.2-94%	1569:1584	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	5	30	theme	defined	642:648	arg1	decrease					659:666	defined as ≥ 50% decrease	642:666	defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS)	642:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	7	31	theme	as	1035:1036	arg1	4 M					1030:1032	25 F; 4 M	1024:1032	25 F; 4 M	1024:1032	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	31	theme	as	1035:1036	arg1	non-responders					1038:1051	29 (25 F; 4 M) as non-responders	1020:1051	29 (25 F; 4 M) as non-responders	1020:1051	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	11	32	theme	CI	1573:1574	arg1	%					1584:1584	95% CI [61.2-94%	1569:1584	95% CI [61.2-94%	1569:1584	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	0	33	theme	Irritable	121:129	arg1	Syndrome					137:144	Irritable Bowel Syndrome	121:144	Irritable Bowel Syndrome	121:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	7	34	theme	F	953:953	arg1	7 M					956:958	54 F; 7 M	950:958	54 F; 7 M	950:958	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	34	theme	F	953:953	arg1	patients					940:947	Sixty-one patients	930:947	Sixty-one patients (54 F; 7 M)	930:959	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	9	35	theme	selected	1327:1334	arg1	marker					1346:1351	each selected bacterial marker	1322:1351	each selected bacterial marker	1322:1351	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	0	36	theme	Microbiota	14:23	arg1	Composition					25:35	Gut Microbiota Composition	10:35	Gut Microbiota Composition	10:35	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	0	36	theme	Microbiota	14:23	arg1	Indicator					43:51	an Indicator	40:51	an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	40:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	1	37	theme	irritable	280:288	arg1	IBS					306:308	IBS	306:308	IBS	306:308	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	37	theme	irritable	280:288	arg1	syndrome					296:303	irritable bowel syndrome	280:303	irritable bowel syndrome (IBS)	280:309	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	7	38	theme	F	983:983	arg1	3 M					986:988	29 F; 3 M	980:988	29 F; 3 M	980:988	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	38	theme	F	983:983	arg1	32					976:977	32	976:977	32	976:977	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	1	39	dep	BACKGROUND	147:156	arg1	diet					160:163	A diet	158:163	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs)	147:254	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	3	40	theme	symptom	464:470	arg1	response					472:479	symptom response	464:479	symptom response to a low-FODMAP diet in patients with IBS	464:521	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	1	41	theme	bowel	290:294	arg1	IBS					306:308	IBS	306:308	IBS	306:308	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	41	theme	bowel	290:294	arg1	syndrome					296:303	irritable bowel syndrome	280:303	irritable bowel syndrome (IBS)	280:309	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	9	42	theme	median	1157:1162	arg1	values					1164:1169	median values	1157:1169	median values (used as cutoff) of responders for these 10 bacterial markers	1157:1231	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	3	43	theme	gut	425:427	arg1	composition					439:449	gut microbial composition	425:449	gut microbial composition	425:449	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	1	44	theme	syndrome	296:303	arg1	symptoms					268:275	symptoms	268:275	symptoms of irritable bowel syndrome (IBS)	268:309	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	0	45	theme	Bowel	131:135	arg1	Syndrome					137:144	Irritable Bowel Syndrome	121:144	Irritable Bowel Syndrome	121:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	6	46	theme	bacterial	840:848	arg1	markers					850:856	54 bacterial markers	837:856	54 bacterial markers targeting more than 300 bacteria at different taxonomic levels	837:919	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	12	47	theme	FODMAP	1736:1741	arg1	restriction					1743:1753	dietary FODMAP restriction	1728:1753	dietary FODMAP restriction	1728:1753	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	10	48	from	10	1431:1432	arg1	RI					1418:1419	an RI	1415:1419	an RI from 0 to 10	1415:1432	These points were summed up, giving an RI from 0 to 10.
29302878	5	49	from	decrease	659:666	arg1	IBS-SSS					700:706	IBS-SSS	700:706	IBS-SSS	700:706	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	5	49	from	decrease	659:666	arg1	scores					692:697	IBS symptom severity scores	671:697	IBS symptom severity scores (IBS-SSS)	671:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	1	50	from	polyols	238:244	arg1	low					165:167	low	165:167	low	165:167	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	12	51	theme	Gut	1600:1602	arg1	composition					1614:1624	Gut microbial composition	1600:1624	CONCLUSIONS Gut microbial composition	1588:1624	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	1	52	from	monosaccharides	217:231	arg1	low					165:167	low	165:167	low	165:167	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	2	53	theme	nutritional	321:331	arg1	counseling					333:342	nutritional counseling	321:342	nutritional counseling	321:342	However, nutritional counseling is resource-demanding and not all patients will benefit.
29302878	5	54	theme	symptom	675:681	arg1	IBS-SSS					700:706	IBS-SSS	700:706	IBS-SSS	700:706	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	5	54	theme	symptom	675:681	arg1	scores					692:697	IBS symptom severity scores	671:697	IBS symptom severity scores (IBS-SSS)	671:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	11	55	with	Patients	1435:1442	arg1	RI > 3					1449:1454	RI > 3	1449:1454	RI > 3	1449:1454	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	7	56	theme	Sixty-one	930:938	arg1	7 M					956:958	54 F; 7 M	950:958	54 F; 7 M	950:958	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	7	56	theme	Sixty-one	930:938	arg1	patients					940:947	Sixty-one patients	930:947	Sixty-one patients (54 F; 7 M)	930:959	RESULTS Sixty-one patients (54 F; 7 M) were included: 32 (29 F; 3 M) classified as responders and 29 (25 F; 4 M) as non-responders.
29302878	9	57	theme	bacterial	1336:1344	arg1	marker					1346:1351	each selected bacterial marker	1322:1351	each selected bacterial marker	1322:1351	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	3	58	theme	low-FODMAP	486:495	arg1	diet					497:500	a low-FODMAP diet	484:500	a low-FODMAP diet	484:500	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	4	59	theme	METHODS	524:530	arg1	Patients					532:539	METHODS Patients	524:539	METHODS Patients	524:539	METHODS Patients were recruited consecutively to participate in a 4-week FODMAP-restricted diet.
29302878	5	60	theme	severity	683:690	arg1	IBS-SSS					700:706	IBS-SSS	700:706	IBS-SSS	700:706	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	5	60	theme	severity	683:690	arg1	scores					692:697	IBS symptom severity scores	671:697	IBS symptom severity scores (IBS-SSS)	671:707	Response to diet was defined as ≥ 50% decrease in IBS symptom severity scores (IBS-SSS) compared to baseline.
29302878	3	61	from	response	472:479	arg1	patients					505:512	patients	505:512	patients with IBS	505:521	AIMS To explore whether gut microbial composition may identify symptom response to a low-FODMAP diet in patients with IBS.
29302878	11	62	dep	respond	1484:1490	arg1	CI					1508:1509	OR = 5.05, 95% CI [1.58; 16.10]	1493:1523	CI	1508:1509	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	1	63	from	disaccharides	202:214	arg1	low					165:167	low	165:167	low	165:167	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	9	64	dep	values	1164:1169	arg1	used					1172:1175	used	1172:1175	used as cutoff	1172:1185	Based on median values (used as cutoff) of responders for these 10 bacterial markers, we constructed a Response Index (RI): Each patient was given a point when the value for each selected bacterial marker differed from the cutoff.
29302878	12	65	theme	microbial	1604:1612	arg1	composition					1614:1624	Gut microbial composition	1600:1624	CONCLUSIONS Gut microbial composition	1588:1624	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	0	66	from	Restriction	92:102	arg1	Patients					107:114	Patients	107:114	Patients with Irritable Bowel Syndrome	107:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	6	67	theme	Fecal	731:735	arg1	microbiota					737:746	Fecal microbiota	731:746	Fecal microbiota	731:746	Fecal microbiota were analyzed by a commercially available method (the GA-map™ Dysbiosis Test), assessing 54 bacterial markers targeting more than 300 bacteria at different taxonomic levels.
29302878	4	68	theme	FODMAP-restricted	597:613	arg1	diet					615:618	a 4-week FODMAP-restricted diet	588:618	a 4-week FODMAP-restricted diet	588:618	METHODS Patients were recruited consecutively to participate in a 4-week FODMAP-restricted diet.
29302878	0	69	theme	Response	65:72	arg1	Composition					25:35	Gut Microbiota Composition	10:35	Gut Microbiota Composition	10:35	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	0	69	theme	Response	65:72	arg1	Indicator					43:51	an Indicator	40:51	an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	40:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	11	70	dep	%	1566:1566	arg1	%					1584:1584	95% CI [61.2-94%	1569:1584	95% CI [61.2-94%	1569:1584	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	11	71	theme	%	1506:1506	arg1	CI					1508:1509	OR = 5.05, 95% CI [1.58; 16.10]	1493:1523	CI	1508:1509	Patients with RI > 3 were 5 times more likely to respond (OR = 5.05, 95% CI [1.58; 16.10]), and the probability to respond was 83.4%, 95% CI [61.2-94%].
29302878	1	72	from	oligosaccharides	184:199	arg1	low					165:167	low	165:167	low	165:167	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	12	73	dep	CONCLUSIONS	1588:1598	arg1	composition					1614:1624	Gut microbial composition	1600:1624	CONCLUSIONS Gut microbial composition	1588:1624	CONCLUSIONS Gut microbial composition, assessed by using a new RI, may constitute a tool to identify patients that are likely to respond to dietary FODMAP restriction.
29302878	0	74	theme	Clinical	56:63	arg1	Response					65:72	Clinical Response	56:72	Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome	56:144	Exploring Gut Microbiota Composition as an Indicator of Clinical Response to Dietary FODMAP Restriction in Patients with Irritable Bowel Syndrome.
29302878	1	75	from	low	165:167	arg1	polyols					238:244	polyols	238:244	polyols	238:244	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	75	from	low	165:167	arg1	disaccharides					202:214	disaccharides	202:214	disaccharides	202:214	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	75	from	low	165:167	arg1	oligosaccharides					184:199	fermentable oligosaccharides	172:199	fermentable oligosaccharides	172:199	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	75	from	low	165:167	arg1	FODMAPs					247:253	FODMAPs	247:253	FODMAPs	247:253	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
29302878	1	75	from	low	165:167	arg1	monosaccharides					217:231	monosaccharides	217:231	monosaccharides	217:231	BACKGROUND A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) may relieve symptoms of irritable bowel syndrome (IBS).
31163085	0	0	theme	association	79:89	arg1	study					91:95	genome-wide association study	67:95	genome-wide association study	67:95	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	3	1	theme	pharmaceutical	337:350	arg1	applications					352:363	diagnostic and pharmaceutical applications	322:363	diagnostic and pharmaceutical applications	322:363	Defining genetic factors altering glycosylation may provide a basis for novel approaches to diagnostic and pharmaceutical applications.
31163085	3	2	theme	genetic	239:245	arg1	factors					247:253	genetic factors	239:253	genetic factors altering glycosylation	239:276	Defining genetic factors altering glycosylation may provide a basis for novel approaches to diagnostic and pharmaceutical applications.
31163085	9	3	theme	protein	1146:1152	arg1	HNF1A					1168:1172	HNF1A	1168:1172	HNF1A	1168:1172	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	3	theme	protein	1146:1152	arg1	fucosylation					1154:1165	plasma protein fucosylation	1139:1165	plasma protein fucosylation (HNF1A)	1139:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	5	4	theme	glycan	678:683	arg1	traits					685:690	113 glycan traits	674:690	113 glycan traits	674:690	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	0	5	theme	genome-wide	67:77	arg1	study					91:95	genome-wide association study	67:95	genome-wide association study	67:95	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	4	6	theme	blood	426:430	arg1	composition					449:459	the human blood plasma N-glycome composition	416:459	the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	416:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	7	theme	human	420:424	arg1	composition					449:459	the human blood plasma N-glycome composition	416:459	the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	416:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	12	8	theme	research	1498:1505	arg1	area					1507:1510	this research area	1493:1510	this research area	1493:1510	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	8	9	dep	an	861:862	arg1	overlap					864:870	overlap	864:870	overlap in genetic control between total plasma protein and IgG glycosylation	864:940	This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation.
31163085	4	10	theme	Performance	500:510	arg1	UPLC					535:538	UPLC	535:538	UPLC	535:538	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	10	theme	Performance	500:510	arg1	Chromatography					519:532	Ultra Performance Liquid Chromatography	494:532	Ultra Performance Liquid Chromatography (UPLC) technology	494:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	6	11	theme	human	775:779	arg1	autosomes					781:789	human autosomes	775:789	human autosomes	775:789	We studied associations between these traits and genetic polymorphisms located on human autosomes.
31163085	4	12	theme	N-glycome	439:447	arg1	composition					449:459	the human blood plasma N-glycome composition	416:459	the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	416:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	10	13	theme	process	1276:1282	arg1	aspects					1251:1257	other more complex aspects	1232:1257	other more complex aspects of glycosylation process	1232:1282	However, we also found loci that could possibly reflect other more complex aspects of glycosylation process.
31163085	5	14	theme	additional	623:632	arg1	77					634:635	an additional 77	620:635	an additional 77	620:635	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	4	15	dep	3811	470:473	arg1	to					467:468	to	467:468	to	467:468	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	9	16	theme	loci	968:971	arg1	majority					947:954	The majority	943:954	The majority of revealed loci	943:971	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	4	17	theme	Ultra	494:498	arg1	UPLC					535:538	UPLC	535:538	UPLC	535:538	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	17	theme	Ultra	494:498	arg1	Chromatography					519:532	Ultra Performance Liquid Chromatography	494:532	Ultra Performance Liquid Chromatography (UPLC) technology	494:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	5	18	theme	derived	637:643	arg1	traits					645:650	an additional 77 derived traits	620:650	an additional 77 derived traits leading to a total of 113 glycan traits	620:690	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	4	19	theme	plasma	432:437	arg1	composition					449:459	the human blood plasma N-glycome composition	416:459	the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	416:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	8	20	theme	genetic	875:881	arg1	control					883:889	genetic control	875:889	genetic control between total plasma protein and IgG glycosylation	875:940	This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation.
31163085	9	21	theme	fucosylation	1154:1165	arg1	regulator					1126:1134	a known regulator	1118:1134	a known regulator of plasma protein fucosylation (HNF1A)	1118:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	21	theme	fucosylation	1154:1165	arg1	glycosylation					1030:1042	glycosylation	1030:1042	glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5)	1030:1112	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	4	22	theme	Chromatography	519:532	arg1	technology					541:550	Ultra Performance Liquid Chromatography (UPLC) technology	494:550	Ultra Performance Liquid Chromatography (UPLC) technology	494:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	9	23	dep	glycosylation	1030:1042	arg1	MGAT3					1097:1101	MGAT3	1097:1101	MGAT3	1097:1101	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	FUT3/FUT6					1045:1053	FUT3/FUT6	1045:1053	FUT3/FUT6	1045:1053	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	MGAT5					1107:1111	MGAT5	1107:1111	MGAT5	1107:1111	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	FUT8					1056:1059	FUT8	1056:1059	FUT8	1056:1059	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	B4GALT1					1079:1085	B4GALT1	1079:1085	B4GALT1	1079:1085	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	ST3GAL4					1088:1094	ST3GAL4	1088:1094	ST3GAL4	1088:1094	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	B3GAT1					1062:1067	B3GAT1	1062:1067	B3GAT1	1062:1067	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	23	dep	glycosylation	1030:1042	arg1	ST6GAL1					1070:1076	ST6GAL1	1070:1076	ST6GAL1	1070:1076	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	8	24	theme	total	899:903	arg1	protein					912:918	total plasma protein	899:918	total plasma protein	899:918	This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation.
31163085	4	25	theme	Liquid	512:517	arg1	UPLC					535:538	UPLC	535:538	UPLC	535:538	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	25	theme	Liquid	512:517	arg1	Chromatography					519:532	Ultra Performance Liquid Chromatography	494:532	Ultra Performance Liquid Chromatography (UPLC) technology	494:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	26	theme	association	395:405	arg1	study					407:411	a genome-wide association study	381:411	a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	381:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	9	27	contain	contained	973:981	arg2	genes					983:987	genes	983:987	genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A)	983:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	27	contain	contained	973:981	arg1	majority					947:954	The majority	943:954	The majority of revealed loci	943:971	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	27	contain	contained	973:981	arg2	enzymes					1001:1007	enzymes	1001:1007	enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A)	1001:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	5	28	link	derived	637:643	arg1	traits					645:650	an additional 77 derived traits	620:650	an additional 77 derived traits leading to a total of 113 glycan traits	620:690	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	12	29	from	studies	1482:1488	arg1	area					1507:1510	this research area	1493:1510	this research area	1493:1510	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	9	30	gly	glycosylation	1030:1042	arg1	HNF1A					1168:1172	HNF1A	1168:1172	HNF1A	1168:1172	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	30	gly	glycosylation	1030:1042	arg1	fucosylation					1154:1165	plasma protein fucosylation	1139:1165	plasma protein fucosylation (HNF1A)	1139:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	11	31	theme	genomic	1296:1302	arg1	annotation					1304:1313	Functional genomic annotation	1285:1313	Functional genomic annotation	1285:1313	Functional genomic annotation suggested the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1.
31163085	4	32	from	study	407:411	arg1	people					475:480	up to 3811 people	464:480	up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	464:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	8	33	theme	plasma	905:910	arg1	protein					912:918	total plasma protein	899:918	total plasma protein	899:918	This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation.
31163085	0	34	theme	genetic	13:19	arg1	control					21:27	the genetic control	9:27	the genetic control of human blood plasma N-glycome	9:59	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	3	35	theme	novel	302:306	arg1	approaches					308:317	novel approaches	302:317	novel approaches to diagnostic and pharmaceutical applications	302:363	Defining genetic factors altering glycosylation may provide a basis for novel approaches to diagnostic and pharmaceutical applications.
31163085	8	36	theme	IgG	924:926	arg1	glycosylation					928:940	IgG glycosylation	924:940	IgG glycosylation	924:940	This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation.
31163085	0	37	theme	human	32:36	arg1	N-glycome					51:59	human blood plasma N-glycome	32:59	human blood plasma N-glycome	32:59	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	4	38	from	people	475:480	arg1	study					407:411	a genome-wide association study	381:411	a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	381:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	10	39	theme	glycosylation	1262:1274	arg1	process					1276:1282	glycosylation process	1262:1282	glycosylation process	1262:1282	However, we also found loci that could possibly reflect other more complex aspects of glycosylation process.
31163085	9	40	theme	known	1120:1124	arg1	regulator					1126:1134	a known regulator	1118:1134	a known regulator of plasma protein fucosylation (HNF1A)	1118:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	11	41	theme	genes	1345:1349	arg1	role					1329:1332	the role	1325:1332	the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1	1325:1398	Functional genomic annotation suggested the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1.
31163085	12	42	theme	functional	1471:1480	arg1	studies					1482:1488	further functional studies	1463:1488	further functional studies in this research area	1463:1510	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	10	43	theme	complex	1243:1249	arg1	aspects					1251:1257	other more complex aspects	1232:1257	other more complex aspects of glycosylation process	1232:1282	However, we also found loci that could possibly reflect other more complex aspects of glycosylation process.
31163085	0	44	theme	plasma	44:49	arg1	N-glycome					51:59	human blood plasma N-glycome	32:59	human blood plasma N-glycome	32:59	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	3	45	theme	diagnostic	322:331	arg1	applications					352:363	diagnostic and pharmaceutical applications	322:363	diagnostic and pharmaceutical applications	322:363	Defining genetic factors altering glycosylation may provide a basis for novel approaches to diagnostic and pharmaceutical applications.
31163085	12	46	theme	further	1463:1469	arg1	studies					1482:1488	further functional studies	1463:1488	further functional studies in this research area	1463:1510	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	5	47	theme	original	574:581	arg1	traits					583:588	the 36 original traits	567:588	the 36 original traits measured by UPLC	567:605	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	11	48	theme	Functional	1285:1294	arg1	annotation					1304:1313	Functional genomic annotation	1285:1313	Functional genomic annotation	1285:1313	Functional genomic annotation suggested the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1.
31163085	0	49	theme	blood	38:42	arg1	N-glycome					51:59	human blood plasma N-glycome	32:59	human blood plasma N-glycome	32:59	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	5	50	theme	traits	685:690	arg1	total					665:669	a total	663:669	a total of 113 glycan traits	663:690	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	2	51	theme	diseases	220:227	arg1	number					204:209	a number	202:209	a number of human diseases	202:227	Glycosylation is associated with a number of human diseases.
31163085	4	52	theme	composition	449:459	arg1	study					407:411	a genome-wide association study	381:411	a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	381:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	4	53	theme	genome-wide	383:393	arg1	study					407:411	a genome-wide association study	381:411	a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	381:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	1	54	theme	common	117:122	arg1	modification					143:154	a common post-translational modification	115:154	a common post-translational modification of proteins	115:166	Glycosylation is a common post-translational modification of proteins.
31163085	1	54	theme	common	117:122	arg1	Glycosylation					98:110	Glycosylation	98:110	Glycosylation	98:110	Glycosylation is a common post-translational modification of proteins.
31163085	12	55	dep	hypotheses	1405:1414	arg1	we					1416:1417	we	1416:1417	we	1416:1417	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	2	56	theme	human	214:218	arg1	diseases					220:227	human diseases	214:227	human diseases	214:227	Glycosylation is associated with a number of human diseases.
31163085	6	57	theme	located	764:770	arg1	traits					731:736	these traits	725:736	these traits	725:736	We studied associations between these traits and genetic polymorphisms located on human autosomes.
31163085	1	58	theme	post-translational	124:141	arg1	modification					143:154	a common post-translational modification	115:154	a common post-translational modification of proteins	115:166	Glycosylation is a common post-translational modification of proteins.
31163085	1	58	theme	post-translational	124:141	arg1	Glycosylation					98:110	Glycosylation	98:110	Glycosylation	98:110	Glycosylation is a common post-translational modification of proteins.
31163085	11	59	theme	several	1337:1343	arg1	genes					1345:1349	several genes	1337:1349	several genes	1337:1349	Functional genomic annotation suggested the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1.
31163085	4	60	from	composition	449:459	arg1	people					475:480	up to 3811 people	464:480	up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology	464:550	Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology.
31163085	12	61	theme	starting	1444:1451	arg1	hypotheses					1405:1414	The hypotheses	1401:1414	The hypotheses we generated	1401:1427	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	12	61	theme	starting	1444:1451	arg1	point					1453:1457	a starting point	1442:1457	a starting point for further functional studies in this research area	1442:1510	The hypotheses we generated may serve as a starting point for further functional studies in this research area.
31163085	5	62	dep	derived	637:643	arg1	77					634:635	an additional 77	620:635	an additional 77	620:635	Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits.
31163085	6	63	theme	genetic	742:748	arg1	polymorphisms					750:762	genetic polymorphisms	742:762	genetic polymorphisms	742:762	We studied associations between these traits and genetic polymorphisms located on human autosomes.
31163085	0	64	theme	N-glycome	51:59	arg1	control					21:27	the genetic control	9:27	the genetic control of human blood plasma N-glycome	9:59	Defining the genetic control of human blood plasma N-glycome using genome-wide association study.
31163085	9	65	theme	plasma	1139:1144	arg1	HNF1A					1168:1172	HNF1A	1168:1172	HNF1A	1168:1172	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	9	65	theme	plasma	1139:1144	arg1	fucosylation					1154:1165	plasma protein fucosylation	1139:1165	plasma protein fucosylation (HNF1A)	1139:1173	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
31163085	1	66	theme	proteins	159:166	arg1	modification					143:154	a common post-translational modification	115:154	a common post-translational modification of proteins	115:166	Glycosylation is a common post-translational modification of proteins.
31163085	1	66	theme	proteins	159:166	arg1	Glycosylation					98:110	Glycosylation	98:110	Glycosylation	98:110	Glycosylation is a common post-translational modification of proteins.
31163085	10	67	theme	other	1232:1236	arg1	aspects					1251:1257	other more complex aspects	1232:1257	other more complex aspects of glycosylation process	1232:1282	However, we also found loci that could possibly reflect other more complex aspects of glycosylation process.
31163085	9	68	theme	revealed	959:966	arg1	loci					968:971	revealed loci	959:971	revealed loci	959:971	The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A).
30372992	0	0	theme	digestible	97:106	arg1	starch					108:113	slowly digestible starch	90:113	slowly digestible starch	90:113	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	6	1	theme	driven	1144:1149	arg1	SDS					1151:1153	structurally driven SDS	1131:1153	structurally driven SDS	1131:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	3	2	theme	EB	582:583	arg1	crumbs					585:590	EB crumbs	582:590	EB crumbs (fully gelatinized)	582:610	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	1	3	theme	native	161:166	arg1	starch					198:203	native (NB) and extruded (EB) banana starch	161:203	native (NB) and extruded (EB) banana starch	161:203	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	4	theme	1:1	212:214	arg1	composite					246:254	extruded banana starch composite	223:254	a 1:1 native:extruded banana starch composite (MB)	210:259	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	4	theme	1:1	212:214	arg1	MB					257:258	MB	257:258	MB	257:258	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	3	5	from	%	566:566	arg1	NB					571:572	NB	571:572	NB	571:572	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	4	6	theme	amylopectin	732:742	arg1	branches					744:751	amylopectin branches	732:751	amylopectin branches	732:751	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	1	7	theme	native	216:221	arg1	composite					246:254	extruded banana starch composite	223:254	a 1:1 native:extruded banana starch composite (MB)	210:259	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	7	theme	native	216:221	arg1	MB					257:258	MB	257:258	MB	257:258	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	8	theme	starch	282:287	arg1	digestibility					289:301	the starch digestibility	278:301	the starch digestibility of bread crumb	278:316	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	6	9	theme	size	1012:1015	arg1	reduction					1017:1025	a molecular size reduction	1000:1025	a molecular size reduction	1000:1025	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	9	theme	size	1012:1015	arg1	strategy					1032:1039	a strategy	1030:1039	a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS	1030:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	10	theme	molecular	1002:1010	arg1	reduction					1017:1025	a molecular size reduction	1000:1025	a molecular size reduction	1000:1025	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	10	theme	molecular	1002:1010	arg1	strategy					1032:1039	a strategy	1030:1039	a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS	1030:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	11	theme	rich	1123:1126	arg1	products					1114:1121	highly gelatinized baked products	1089:1121	highly gelatinized baked products rich in structurally driven SDS	1089:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	2	12	theme	molecular	368:376	arg1	weight					378:383	the molecular weight	364:383	the molecular weight of banana starch	364:400	During extrusion, the molecular weight of banana starch was reduced from 2.75 × 108 to 4.48 × 106 g/mol (HPSEC-MALS-RI).
30372992	1	13	theme	extruded	223:230	arg1	composite					246:254	extruded banana starch composite	223:254	a 1:1 native:extruded banana starch composite (MB)	210:259	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	13	theme	extruded	223:230	arg1	MB					257:258	MB	257:258	MB	257:258	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	0	14	theme	gelatinized	124:134	arg1	crumb					142:146	fully gelatinized bread crumb	118:146	fully gelatinized bread crumb	118:146	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	4	15	dep	those	765:769	arg1	especially					754:763	especially	754:763	especially	754:763	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	3	16	from	MB	575:576	arg1	NB					571:572	NB	571:572	NB	571:572	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	1	17	theme	bread	306:310	arg1	crumb					312:316	bread crumb	306:316	bread crumb	306:316	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	4	18	theme	structures	698:707	arg1	formation					670:678	the formation	666:678	the formation of supramolecular structures upon storage involving amylopectin branches	666:751	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	4	19	theme	DSC	627:629	arg1	data					631:634	DSC data	627:634	DSC data	627:634	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	1	20	theme	crumb	312:316	arg1	digestibility					289:301	the starch digestibility	278:301	the starch digestibility of bread crumb	278:316	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	6	21	theme	gelatinized	1096:1106	arg1	products					1114:1121	highly gelatinized baked products	1089:1121	highly gelatinized baked products rich in structurally driven SDS	1089:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	1	22	dep	native	161:166	arg1	NB					169:170	NB	169:170	NB	169:170	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	0	23	theme	Banana	0:5	arg1	starch					7:12	Banana starch	0:12	Banana starch	0:12	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	1	24	theme	banana	232:237	arg1	composite					246:254	extruded banana starch composite	223:254	a 1:1 native:extruded banana starch composite (MB)	210:259	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	24	theme	banana	232:237	arg1	MB					257:258	MB	257:258	MB	257:258	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	5	25	theme	hedonic	811:817	arg1	test					827:830	The hedonic sensory test	807:830	The hedonic sensory test	807:830	The hedonic sensory test showed no differences in overall liking between MB, EB and control, validating feasibility of including banana in the formulation.
30372992	1	26	theme	extruded	177:184	arg1	starch					198:203	native (NB) and extruded (EB) banana starch	161:203	native (NB) and extruded (EB) banana starch	161:203	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	0	27	theme	molecular	18:26	arg1	fragmentation					34:46	molecular shear fragmentation	18:46	molecular shear fragmentation	18:46	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	1	28	theme	starch	239:244	arg1	composite					246:254	extruded banana starch composite	223:254	a 1:1 native:extruded banana starch composite (MB)	210:259	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	28	theme	starch	239:244	arg1	MB					257:258	MB	257:258	MB	257:258	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	3	29	theme	digestible	491:500	arg1	starch					502:507	a slowly digestible starch	482:507	a slowly digestible starch (SDS) increase	482:522	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	3	29	theme	digestible	491:500	arg1	SDS					510:512	SDS	510:512	SDS	510:512	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	1	30	theme	composite	246:254	arg1	crust					322:326	crust	322:326	crust	322:326	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	30	theme	composite	246:254	arg1	role					153:156	The role	149:156	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb	149:316	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	0	31	theme	bread	136:140	arg1	crumb					142:146	fully gelatinized bread crumb	118:146	fully gelatinized bread crumb	118:146	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	3	32	theme	starch	502:507	arg1	increase					515:522	a slowly digestible starch (SDS) increase	482:522	a slowly digestible starch (SDS) increase	482:522	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	0	33	dep	increase	61:68	arg1	driven					83:88	driven	83:88	increase structurally driven slowly digestible starch in fully gelatinized bread crumb	61:146	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	4	34	dep	attributed	636:645	arg1	those					765:769	those	765:769	those	765:769	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	0	35	theme	shear	28:32	arg1	fragmentation					34:46	molecular shear fragmentation	18:46	molecular shear fragmentation	18:46	Banana starch and molecular shear fragmentation dramatically increase structurally driven slowly digestible starch in fully gelatinized bread crumb.
30372992	1	36	dep	extruded	177:184	arg1	EB					187:188	EB	187:188	EB	187:188	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	37	theme	banana	191:196	arg1	starch					198:203	native (NB) and extruded (EB) banana starch	161:203	native (NB) and extruded (EB) banana starch	161:203	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	2	38	theme	starch	395:400	arg1	weight					378:383	the molecular weight	364:383	the molecular weight of banana starch	364:400	During extrusion, the molecular weight of banana starch was reduced from 2.75 × 108 to 4.48 × 106 g/mol (HPSEC-MALS-RI).
30372992	6	39	from	SDS	1151:1153	arg1	rich					1123:1126	rich	1123:1126	rich	1123:1126	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	4	40	theme	supramolecular	683:696	arg1	structures					698:707	supramolecular structures	683:707	supramolecular structures	683:707	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	2	41	theme	banana	388:393	arg1	starch					395:400	banana starch	388:400	banana starch	388:400	During extrusion, the molecular weight of banana starch was reduced from 2.75 × 108 to 4.48 × 106 g/mol (HPSEC-MALS-RI).
30372992	3	42	dep	crumbs	585:590	arg1	gelatinized					599:609	gelatinized	599:609	gelatinized	599:609	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
30372992	6	43	theme	baked	1108:1112	arg1	products					1114:1121	highly gelatinized baked products	1089:1121	highly gelatinized baked products rich in structurally driven SDS	1089:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	44	theme	selected	1056:1063	arg1	starches					1065:1072	selected starches	1056:1072	selected starches that result in highly gelatinized baked products rich in structurally driven SDS	1056:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	5	45	theme	overall	857:863	arg1	liking					865:870	overall liking	857:870	overall liking	857:870	The hedonic sensory test showed no differences in overall liking between MB, EB and control, validating feasibility of including banana in the formulation.
30372992	5	46	theme	sensory	819:825	arg1	test					827:830	The hedonic sensory test	807:830	The hedonic sensory test	807:830	The hedonic sensory test showed no differences in overall liking between MB, EB and control, validating feasibility of including banana in the formulation.
30372992	1	47	theme	starch	198:203	arg1	crust					322:326	crust	322:326	crust	322:326	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	1	47	theme	starch	198:203	arg1	role					153:156	The role	149:156	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb	149:316	The role of native (NB) and extruded (EB) banana starch, and a 1:1 native:extruded banana starch composite (MB), in slowing down the starch digestibility of bread crumb and crust was investigated.
30372992	4	48	theme	fragmented	776:785	arg1	amylopectin					787:797	fragmented amylopectin	776:797	fragmented amylopectin	776:797	DSC data attributed this occurrence to the formation of supramolecular structures upon storage involving amylopectin branches (especially those from fragmented amylopectin in EB).
30372992	6	49	theme	first	971:975	arg1	time					977:980	the first time	967:980	the first time	967:980	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	6	50	from	rich	1123:1126	arg1	SDS					1151:1153	structurally driven SDS	1131:1153	structurally driven SDS	1131:1153	For the first time, this study shows a molecular size reduction as a strategy to manufacture selected starches that result in highly gelatinized baked products rich in structurally driven SDS.
30372992	5	51	from	differences	842:852	arg1	liking					865:870	overall liking	857:870	overall liking	857:870	The hedonic sensory test showed no differences in overall liking between MB, EB and control, validating feasibility of including banana in the formulation.
30372992	3	52	from	crumbs	585:590	arg1	NB					571:572	NB	571:572	NB	571:572	Results showed a slowly digestible starch (SDS) increase from 1.09% (control) to 4.2, 6.6, and 7.76% in NB, MB and EB crumbs (fully gelatinized), respectively.
31766842	0	0	theme	Sensitive	87:95	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	7	1	with	tests	902:906	arg1	radius					931:936	a minimum bending radius	913:936	a minimum bending radius of 1.5 mm	913:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	11	2	theme	calibration	1286:1296	arg1	curve					1298:1302	a calibration curve	1284:1302	a calibration curve	1284:1302	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	8	3	theme	ZnO	962:964	arg1	NRs					966:968	ZnO NRs	962:968	ZnO NRs with different lengths	962:991	In addition, ZnO NRs with different lengths were synthesized.
31766842	4	4	theme	ZnO	518:520	arg1	NRs					522:524	brittle ZnO NRs	510:524	brittle ZnO NRs	510:524	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	5	5	theme	CNF/ZnO	646:652	arg1	film					671:674	the CNF/ZnO NR nanocomposite film	642:674	the CNF/ZnO NR nanocomposite film	642:674	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	8	6	theme	different	975:983	arg1	lengths					985:991	different lengths	975:991	different lengths	975:991	In addition, ZnO NRs with different lengths were synthesized.
31766842	1	7	theme	Organic	168:174	arg1	nanomaterials					206:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials were synthesized and combined into a nanocomposite film for a wearable sensor.
31766842	7	8	theme	bending	894:900	arg1	tests					902:906	repeated bending tests	885:906	repeated bending tests with a minimum bending radius of 1.5 mm	885:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	4	9	theme	CNFs	574:577	arg1	network					563:569	the flexible network	550:569	the flexible network of CNFs	550:577	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	0	10	theme	Reliable	98:105	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	4	11	theme	brittle	510:516	arg1	NRs					522:524	brittle ZnO NRs	510:524	brittle ZnO NRs	510:524	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	5	12	theme	NR	654:655	arg1	film					671:674	the CNF/ZnO NR nanocomposite film	642:674	the CNF/ZnO NR nanocomposite film	642:674	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	2	13	theme	nanorod	316:322	arg1	synthesis					329:337	Reproducible ZnO nanorod (NR) synthesis	299:337	Reproducible ZnO nanorod (NR) synthesis	299:337	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	1	14	theme	wearable	282:289	arg1	sensor					291:296	a wearable sensor	280:296	a wearable sensor	280:296	Organic and inorganic one-dimensional nanomaterials were synthesized and combined into a nanocomposite film for a wearable sensor.
31766842	5	15	theme	nanocomposite	657:669	arg1	film					671:674	the CNF/ZnO NR nanocomposite film	642:674	the CNF/ZnO NR nanocomposite film	642:674	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	2	16	theme	ZnO	312:314	arg1	NR					325:326	NR	325:326	NR	325:326	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	2	16	theme	ZnO	312:314	arg1	nanorod					316:322	Reproducible ZnO nanorod	299:322	Reproducible ZnO nanorod (NR) synthesis	299:337	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	9	17	theme	×	1062:1062	arg1	times					1068:1072	2.8 × 103 times	1058:1072	2.8 × 103 times higher photocurrent and responsivity performance	1058:1121	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	0	18	theme	Wireless	108:115	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	13	19	theme	wearable	1465:1472	arg1	sensors					1474:1480	wearable sensors	1465:1480	wearable sensors	1465:1480	These results open an avenue for developing wearable sensors by overcoming the brittleness of inorganic materials.
31766842	2	20	theme	Reproducible	299:310	arg1	NR					325:326	NR	325:326	NR	325:326	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	2	20	theme	Reproducible	299:310	arg1	nanorod					316:322	Reproducible ZnO nanorod	299:322	Reproducible ZnO nanorod (NR) synthesis	299:337	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	7	21	theme	repeated	885:892	arg1	tests					902:906	repeated bending tests	885:906	repeated bending tests with a minimum bending radius of 1.5 mm	885:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	13	22	theme	materials	1525:1533	arg1	brittleness					1500:1510	the brittleness	1496:1510	the brittleness of inorganic materials	1496:1533	These results open an avenue for developing wearable sensors by overcoming the brittleness of inorganic materials.
31766842	4	23	theme	interconnected	483:496	arg1	channels					498:505	The interconnected channels	479:505	The interconnected channels of brittle ZnO NRs	479:524	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	5	24	theme	mechanical	617:626	arg1	properties					628:637	mechanical properties	617:637	mechanical properties	617:637	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	3	25	used	used	436:439	arg2	CNFs					425:428	CNFs	425:428	CNFs	425:428	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	3	25	used	used	436:439	arg2	nanofibers					413:422	Cellulose nanofibers	403:422	Cellulose nanofibers (CNFs)	403:429	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	6	26	theme	interfacial	700:710	arg1	interactions					712:723	The interfacial interactions	696:723	The interfacial interactions between these two nanomaterials	696:755	The interfacial interactions between these two nanomaterials were also studied.
31766842	2	27	theme	water	396:400	arg1	water					396:400	water	396:400	water	396:400	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	2	27	theme	water	396:400	arg1	amount					386:391	an appropriate amount	371:391	an appropriate amount of water	371:400	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	0	28	theme	Organic/Inorganic	3:19	arg1	Nanocomposite					21:33	An Organic/Inorganic Nanocomposite	0:33	An Organic/Inorganic Nanocomposite of Cellulose	0:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	0	28	theme	Organic/Inorganic	3:19	arg1	Cellulose					38:46	Cellulose	38:46	Cellulose	38:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	10	29	theme	sensing	1137:1143	arg1	performance					1145:1155	The humidity sensing performance	1124:1155	The humidity sensing performance of the composite	1124:1172	The humidity sensing performance of the composite was also suggested.
31766842	11	30	theme	NR	1206:1207	arg1	film					1209:1212	The CNF/ZnO NR film	1194:1212	The CNF/ZnO NR film	1194:1212	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	9	31	theme	higher	1074:1079	arg1	times					1068:1072	2.8 × 103 times	1058:1072	2.8 × 103 times higher photocurrent and responsivity performance	1058:1121	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	1	32	theme	one-dimensional	190:204	arg1	nanomaterials					206:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials were synthesized and combined into a nanocomposite film for a wearable sensor.
31766842	4	33	theme	flexible	554:561	arg1	network					563:569	the flexible network	550:569	the flexible network of CNFs	550:577	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	7	34	theme	minimum	915:921	arg1	radius					931:936	a minimum bending radius	913:936	a minimum bending radius of 1.5 mm	913:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	7	35	theme	electrical	844:853	arg1	changes					866:872	no electrical resistance changes	841:872	no electrical resistance changes	841:872	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	2	36	theme	amount	386:391	arg1	addition					359:366	the addition	355:366	the addition of an appropriate amount of water	355:400	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	10	37	theme	humidity	1128:1135	arg1	performance					1145:1155	The humidity sensing performance	1124:1155	The humidity sensing performance of the composite	1124:1172	The humidity sensing performance of the composite was also suggested.
31766842	11	38	theme	electrical	1231:1240	arg1	changes					1249:1255	reasonable electrical signal changes	1220:1255	reasonable electrical signal changes enabling the evaluation of a calibration curve	1220:1302	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	5	39	theme	surface	584:590	arg1	morphology					592:601	surface morphology	584:601	surface morphology	584:601	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	7	40	theme	resistance	855:864	arg1	changes					866:872	no electrical resistance changes	841:872	no electrical resistance changes	841:872	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	3	41	theme	porous	454:459	arg1	formation					468:476	their porous matrix formation	448:476	their porous matrix formation	448:476	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	11	42	theme	signal	1242:1247	arg1	changes					1249:1255	reasonable electrical signal changes	1220:1255	reasonable electrical signal changes enabling the evaluation of a calibration curve	1220:1302	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	11	43	theme	CNF/ZnO	1198:1204	arg1	film					1209:1212	The CNF/ZnO NR film	1194:1212	The CNF/ZnO NR film	1194:1212	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	9	44	theme	responsivity	1098:1109	arg1	performance					1111:1121	responsivity performance	1098:1121	responsivity performance	1098:1121	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	3	45	theme	matrix	461:466	arg1	formation					468:476	their porous matrix formation	448:476	their porous matrix formation	448:476	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	8	46	with	NRs	966:968	arg1	lengths					985:991	different lengths	975:991	different lengths	975:991	In addition, ZnO NRs with different lengths were synthesized.
31766842	11	47	theme	curve	1298:1302	arg1	evaluation					1270:1279	the evaluation	1266:1279	the evaluation of a calibration curve	1266:1302	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	0	48	theme	Multifunctional	131:145	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	12	49	theme	NR	1347:1348	arg1	sensor					1355:1360	a CNF/ZnO NR film sensor	1337:1360	a CNF/ZnO NR film sensor	1337:1360	Finally, a smart band including a CNF/ZnO NR film sensor was fabricated and connected to a smartphone by Bluetooth.
31766842	7	50	theme	bending	923:929	arg1	radius					931:936	a minimum bending radius	913:936	a minimum bending radius of 1.5 mm	913:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	0	51	theme	Cellulose	38:46	arg1	Nanocomposite					21:33	An Organic/Inorganic Nanocomposite	0:33	An Organic/Inorganic Nanocomposite of Cellulose	0:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	0	51	theme	Cellulose	38:46	arg1	Cellulose					38:46	Cellulose	38:46	Cellulose	38:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	10	52	theme	composite	1164:1172	arg1	performance					1145:1155	The humidity sensing performance	1124:1155	The humidity sensing performance of the composite	1124:1172	The humidity sensing performance of the composite was also suggested.
31766842	0	53	theme	Wearable	122:129	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	12	54	theme	CNF/ZnO	1339:1345	arg1	sensor					1355:1360	a CNF/ZnO NR film sensor	1337:1360	a CNF/ZnO NR film sensor	1337:1360	Finally, a smart band including a CNF/ZnO NR film sensor was fabricated and connected to a smartphone by Bluetooth.
31766842	3	55	theme	Cellulose	403:411	arg1	nanofibers					413:422	Cellulose nanofibers	403:422	Cellulose nanofibers (CNFs)	403:429	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	3	55	theme	Cellulose	403:411	arg1	CNFs					425:428	CNFs	425:428	CNFs	425:428	Cellulose nanofibers (CNFs) were used due to their porous matrix formation.
31766842	4	56	theme	NRs	522:524	arg1	channels					498:505	The interconnected channels	479:505	The interconnected channels of brittle ZnO NRs	479:524	The interconnected channels of brittle ZnO NRs were well-fabricated in the flexible network of CNFs.
31766842	5	57	theme	film	671:674	arg1	properties					628:637	mechanical properties	617:637	mechanical properties	617:637	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	5	57	theme	film	671:674	arg1	morphology					592:601	surface morphology	584:601	surface morphology	584:601	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	0	58	dep	Nanofibers	48:57	arg1	Nanocomposite					21:33	An Organic/Inorganic Nanocomposite	0:33	An Organic/Inorganic Nanocomposite of Cellulose	0:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	0	58	dep	Nanofibers	48:57	arg1	Cellulose					38:46	Cellulose	38:46	Cellulose	38:46	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	9	59	dep	higher	1074:1079	arg1	photocurrent					1081:1092	photocurrent	1081:1092	photocurrent	1081:1092	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	9	59	dep	higher	1074:1079	arg1	performance					1111:1121	responsivity performance	1098:1121	responsivity performance	1098:1121	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	11	60	theme	reasonable	1220:1229	arg1	changes					1249:1255	reasonable electrical signal changes	1220:1255	reasonable electrical signal changes enabling the evaluation of a calibration curve	1220:1302	The CNF/ZnO NR film shows reasonable electrical signal changes enabling the evaluation of a calibration curve.
31766842	7	61	theme	nanocomposite	780:792	arg1	film					794:797	The nanocomposite film	776:797	The nanocomposite film	776:797	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	7	61	theme	nanocomposite	780:792	arg1	flexible					815:822	flexible	815:822	flexible	815:822	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	0	62	theme	Sensor	147:152	arg1	Applications					154:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications	80:165	An Organic/Inorganic Nanocomposite of Cellulose Nanofibers and ZnO Nanorods for Highly Sensitive, Reliable, Wireless, and Wearable Multifunctional Sensor Applications.
31766842	12	63	theme	film	1350:1353	arg1	sensor					1355:1360	a CNF/ZnO NR film sensor	1337:1360	a CNF/ZnO NR film sensor	1337:1360	Finally, a smart band including a CNF/ZnO NR film sensor was fabricated and connected to a smartphone by Bluetooth.
31766842	9	64	theme	ZnO	1035:1037	arg1	NRs					1039:1041	longer ZnO NRs	1028:1041	longer ZnO NRs	1028:1041	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	7	65	theme	mm	945:946	arg1	radius					931:936	a minimum bending radius	913:936	a minimum bending radius of 1.5 mm	913:946	The nanocomposite film is sufficiently flexible so that it shows no electrical resistance changes even after repeated bending tests with a minimum bending radius of 1.5 mm.
31766842	13	66	theme	inorganic	1515:1523	arg1	materials					1525:1533	inorganic materials	1515:1533	inorganic materials	1515:1533	These results open an avenue for developing wearable sensors by overcoming the brittleness of inorganic materials.
31766842	9	67	theme	longer	1028:1033	arg1	NRs					1039:1041	longer ZnO NRs	1028:1041	longer ZnO NRs	1028:1041	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	12	68	theme	smart	1316:1320	arg1	band					1322:1325	a smart band	1314:1325	a smart band including a CNF/ZnO NR film sensor	1314:1360	Finally, a smart band including a CNF/ZnO NR film sensor was fabricated and connected to a smartphone by Bluetooth.
31766842	5	69	dep	morphology	592:601	arg1	The					580:582	The	580:582	The	580:582	The surface morphology, thermal, and mechanical properties of the CNF/ZnO NR nanocomposite film were characterized.
31766842	2	70	theme	appropriate	374:384	arg1	water					396:400	water	396:400	water	396:400	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	2	70	theme	appropriate	374:384	arg1	amount					386:391	an appropriate amount	371:391	an appropriate amount of water	371:400	Reproducible ZnO nanorod (NR) synthesis was achieved by the addition of an appropriate amount of water.
31766842	1	71	theme	inorganic	180:188	arg1	nanomaterials					206:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials	168:218	Organic and inorganic one-dimensional nanomaterials were synthesized and combined into a nanocomposite film for a wearable sensor.
31766842	1	72	theme	nanocomposite	257:269	arg1	film					271:274	a nanocomposite film	255:274	a nanocomposite film for a wearable sensor	255:296	Organic and inorganic one-dimensional nanomaterials were synthesized and combined into a nanocomposite film for a wearable sensor.
31766842	9	73	theme	NRs	1039:1041	arg1	CNF					1047:1049	CNF	1047:1049	CNF	1047:1049	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	9	73	theme	NRs	1039:1041	arg1	NRs					1039:1041	longer ZnO NRs	1028:1041	longer ZnO NRs	1028:1041	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
31766842	9	73	theme	NRs	1039:1041	arg1	composite					1015:1023	The composite	1011:1023	The composite of longer ZnO NRs	1011:1041	The composite of longer ZnO NRs and CNF showed 2.8 × 103 times higher photocurrent and responsivity performance.
30065236	1	0	from	indusiata	270:278	arg1	polysaccharides					224:238	polysaccharides	224:238	polysaccharides from the mushroom Dictyophora indusiata	224:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	1	0	from	indusiata	270:278	arg1	activity					212:219	the tremendous biological activity	186:219	the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata	186:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	7	1	theme	integrity	1861:1869	arg1	lowering					1902:1909	lowering	1902:1909	lowering of endotoxin levels	1902:1929	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	1	theme	integrity	1861:1869	arg1	enhancement					1834:1844	the enhancement	1830:1844	the enhancement of gut barrier integrity	1830:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	1	theme	integrity	1861:1869	arg1	reduction					1872:1880	reduction	1872:1880	reduction of inflammation	1872:1896	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	2	theme	gut	1849:1851	arg1	integrity					1861:1869	gut barrier integrity	1849:1869	gut barrier integrity	1849:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	4	3	theme	associated	725:734	arg1	proteins					736:743	tight-junction associated proteins	710:743	tight-junction associated proteins	710:743	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	6	4	theme	endotoxemia	1371:1381	arg1	reduction					1358:1366	the reduction	1354:1366	the reduction of endotoxemia (through lipopolysaccharides (LPSs))	1354:1418	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	4	theme	endotoxemia	1371:1381	arg1	levels					1538:1543	pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels	1424:1543	pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels	1424:1543	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	1	5	theme	tremendous	190:199	arg1	activity					212:219	the tremendous biological activity	186:219	the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata	186:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	6	6	dep	proteins	1605:1612	arg1	occludin					1626:1633	occludin	1626:1633	occludin	1626:1633	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	6	dep	proteins	1605:1612	arg1	claudin-1					1615:1623	claudin-1	1615:1623	claudin-1	1615:1623	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	6	dep	proteins	1605:1612	arg1	proteins					1605:1612	tight-junction associated proteins	1579:1612	tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1579:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	6	dep	proteins	1605:1612	arg1	occludens-1					1647:1657	zonula occludens-1	1640:1657	zonula occludens-1	1640:1657	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	7	theme	associated	1594:1603	arg1	occludin					1626:1633	occludin	1626:1633	occludin	1626:1633	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	7	theme	associated	1594:1603	arg1	claudin-1					1615:1623	claudin-1	1615:1623	claudin-1	1615:1623	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	7	theme	associated	1594:1603	arg1	occludens-1					1647:1657	zonula occludens-1	1640:1657	zonula occludens-1	1640:1657	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	7	theme	associated	1594:1603	arg1	proteins					1605:1612	tight-junction associated proteins	1579:1612	tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1579:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	5	8	theme	various	968:974	arg1	levels					986:991	various taxonomic levels	968:991	various taxonomic levels	968:991	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	6	9	theme	tumor	1451:1455	arg1	TNF-α					1480:1484	TNF-α	1480:1484	TNF-α	1480:1484	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	9	theme	tumor	1451:1455	arg1	alpha					1473:1477	tumor necrosis factor alpha	1451:1477	tumor necrosis factor alpha (TNF-α)	1451:1485	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	3	10	theme	microbial	609:617	arg1	communities					619:629	the microbial communities	605:629	the microbial communities	605:629	Alteration and restoration in the microbial communities were elucidated by the Illumina MiSeq platform.
30065236	1	11	theme	polysaccharides	224:238	arg1	activity					212:219	the tremendous biological activity	186:219	the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata	186:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	7	12	theme	comprehensive	1697:1709	arg1	understanding					1711:1723	a comprehensive understanding	1695:1723	a comprehensive understanding of the protective effects of a DIP	1695:1758	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	1	13	from	role	285:288	arg1	restoration					297:307	the restoration	293:307	the restoration of gut microbiota	293:325	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	3	14	theme	Illumina	654:661	arg1	platform					669:676	the Illumina MiSeq platform	650:676	the Illumina MiSeq platform	650:676	Alteration and restoration in the microbial communities were elucidated by the Illumina MiSeq platform.
30065236	0	15	theme	Mouse	165:169	arg1	Model					171:175	a Mouse Model	163:175	a Mouse Model	163:175	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	5	16	theme	DIP	1141:1143	arg1	administration					1145:1158	DIP administration	1141:1158	DIP administration	1141:1158	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	17	dep	flora	1077:1081	arg1	Enterococcus					1100:1111	Enterococcus	1100:1111	Enterococcus	1100:1111	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	17	dep	flora	1077:1081	arg1	Bacteroides					1118:1128	Bacteroides	1118:1128	Bacteroides	1118:1128	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	17	dep	flora	1077:1081	arg1	Proteobacteria					1084:1097	Proteobacteria	1084:1097	Proteobacteria	1084:1097	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	6	18	theme	zonula	1640:1645	arg1	proteins					1605:1612	tight-junction associated proteins	1579:1612	tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1579:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	18	theme	zonula	1640:1645	arg1	occludens-1					1647:1657	zonula occludens-1	1640:1657	zonula occludens-1	1640:1657	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	5	19	theme	increased	1037:1045	arg1	abundance					1056:1064	increased relative abundance	1037:1064	increased relative abundance	1037:1064	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	4	20	theme	serum/tissue	750:761	arg1	endotoxin					763:771	serum/tissue endotoxin and cytokine levels	750:791	endotoxin	763:771	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	7	21	theme	gut	1782:1784	arg1	microbiota					1786:1795	gut microbiota	1782:1795	gut microbiota	1782:1795	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	5	22	theme	daily	818:822	arg1	administration					829:842	Two-week daily oral administration	809:842	Two-week daily oral administration of clindamycin and metronidazole	809:875	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	2	23	theme	barrier	508:514	arg1	function					516:523	intestinal barrier function	497:523	intestinal barrier function	497:523	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	0	24	theme	Intestinal	94:103	arg1	Dysbiosis					105:113	Antibiotic-Driven Intestinal Dysbiosis	76:113	Antibiotic-Driven Intestinal Dysbiosis	76:113	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	5	25	theme	acid-producing	1250:1263	arg1	Lactobacillaceae					1225:1240	Lactobacillaceae	1225:1240	Lactobacillaceae (lactic acid-producing bacteria)	1225:1273	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	25	theme	acid-producing	1250:1263	arg1	bacteria					1265:1272	lactic acid-producing bacteria	1243:1272	lactic acid-producing bacteria	1243:1272	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	26	theme	altered	994:1000	arg1	ratio					1027:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	27	theme	Two-week	809:816	arg1	administration					829:842	Two-week daily oral administration	809:842	Two-week daily oral administration of clindamycin and metronidazole	809:875	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	2	28	theme	antibiotic-driven	546:562	arg1	dysbiosis					564:572	broad-spectrum antibiotic-driven dysbiosis	531:572	broad-spectrum antibiotic-driven dysbiosis	531:572	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	5	29	theme	oral	824:827	arg1	administration					829:842	Two-week daily oral administration	809:842	Two-week daily oral administration of clindamycin and metronidazole	809:875	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	2	30	theme	D.	401:402	arg1	DIP					430:432	DIP	430:432	DIP	430:432	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	2	30	theme	D.	401:402	arg1	polysaccharide					414:427	D. indusiata polysaccharide	401:427	D. indusiata polysaccharide (DIP)	401:433	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	7	31	theme	effects	1743:1749	arg1	understanding					1711:1723	a comprehensive understanding	1695:1723	a comprehensive understanding of the protective effects of a DIP	1695:1758	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	1	32	theme	gut	312:314	arg1	microbiota					316:325	gut microbiota	312:325	gut microbiota	312:325	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	5	33	theme	harmful	1069:1075	arg1	flora					1077:1081	harmful flora	1069:1081	harmful flora (Proteobacteria, Enterococcus, and Bacteroides)	1069:1129	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	2	34	theme	microbiota	470:479	arg1	composition					481:491	gut microbiota composition	466:491	gut microbiota composition	466:491	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	2	35	theme	present	358:364	arg1	study					366:370	The present study	354:370	The present study	354:370	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	5	36	theme	reduced	889:895	arg1	diversity					907:915	reduced bacterial diversity	889:915	reduced bacterial diversity	889:915	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	1	37	theme	mushroom	249:256	arg1	indusiata					270:278	the mushroom Dictyophora indusiata	245:278	the mushroom Dictyophora indusiata	245:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	7	38	theme	barrier	1853:1859	arg1	integrity					1861:1869	gut barrier integrity	1849:1869	gut barrier integrity	1849:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	0	39	attach	Isolated	17:24	arg1	indusiata					43:51	indusiata	43:51	indusiata	43:51	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	0	39	attach	Isolated	17:24	arg2	Polysaccharide					2:15	A Polysaccharide	0:15	A Polysaccharide Isolated from Dictyophora indusiata	0:51	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	5	40	theme	microbial	949:957	arg1	flora					959:963	the microbial flora	945:963	the microbial flora	945:963	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	6	41	theme	factor	1466:1471	arg1	TNF-α					1480:1484	TNF-α	1480:1484	TNF-α	1480:1484	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	41	theme	factor	1466:1471	arg1	alpha					1473:1477	tumor necrosis factor alpha	1451:1477	tumor necrosis factor alpha (TNF-α)	1451:1485	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	4	42	theme	tight-junction	710:723	arg1	proteins					736:743	tight-junction associated proteins	710:743	tight-junction associated proteins	710:743	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	6	43	theme	proteins	1605:1612	arg1	expression					1565:1574	the increased expression	1551:1574	the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1551:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	1	44	theme	biological	201:210	arg1	activity					212:219	the tremendous biological activity	186:219	the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata	186:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	5	45	dep	resulted	877:884	arg1	ratio					1027:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	45	dep	resulted	877:884	arg1	abundance					1056:1064	increased relative abundance	1037:1064	increased relative abundance	1037:1064	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	45	dep	resulted	877:884	arg1	whereas					1133:1139	whereas	1133:1139	whereas	1133:1139	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	4	46	theme	proteins	736:743	arg1	endotoxin					763:771	serum/tissue endotoxin and cytokine levels	750:791	endotoxin	763:771	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	4	46	theme	proteins	736:743	arg1	levels					786:791	serum/tissue endotoxin and cytokine levels	750:791	levels	786:791	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	4	46	theme	proteins	736:743	arg1	histology					685:693	Colon histology	679:693	Colon histology	679:693	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	4	46	theme	proteins	736:743	arg1	expression					696:705	expression	696:705	expression of tight-junction associated proteins	696:743	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	6	47	theme	necrosis	1457:1464	arg1	TNF-α					1480:1484	TNF-α	1480:1484	TNF-α	1480:1484	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	47	theme	necrosis	1457:1464	arg1	alpha					1473:1477	tumor necrosis factor alpha	1451:1477	tumor necrosis factor alpha (TNF-α)	1451:1485	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	48	theme	tight-junction	1579:1592	arg1	occludin					1626:1633	occludin	1626:1633	occludin	1626:1633	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	48	theme	tight-junction	1579:1592	arg1	claudin-1					1615:1623	claudin-1	1615:1623	claudin-1	1615:1623	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	48	theme	tight-junction	1579:1592	arg1	occludens-1					1647:1657	zonula occludens-1	1640:1657	zonula occludens-1	1640:1657	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	48	theme	tight-junction	1579:1592	arg1	proteins					1605:1612	tight-junction associated proteins	1579:1612	tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1579:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	0	49	from	Dysbiosis	105:113	arg1	Recovery					62:69	Recovery	62:69	Recovery from Antibiotic-Driven Intestinal Dysbiosis	62:113	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	6	50	dep	cytokine	1441:1448	arg1	IL-1β					1530:1534	IL-1β	1530:1534	IL-1β	1530:1534	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	50	dep	cytokine	1441:1448	arg1	interleukin					1488:1498	interleukin 6	1488:1500	interleukin 6 (IL-6)	1488:1507	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	50	dep	cytokine	1441:1448	arg1	interleukin					1514:1524	interleukin 1β	1514:1527	interleukin 1β (IL-1β)	1514:1535	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	50	dep	cytokine	1441:1448	arg1	alpha					1473:1477	tumor necrosis factor alpha	1451:1477	tumor necrosis factor alpha (TNF-α)	1451:1485	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	50	dep	cytokine	1441:1448	arg1	TNF-α					1480:1484	TNF-α	1480:1484	TNF-α	1480:1484	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	50	dep	cytokine	1441:1448	arg1	IL-6					1503:1506	IL-6	1503:1506	IL-6	1503:1506	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	6	51	theme	pro-inflammatory	1424:1439	arg1	cytokine					1441:1448	pro-inflammatory cytokine	1424:1448	pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels	1424:1543	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	5	52	theme	beneficial	1197:1206	arg1	flora					1208:1212	beneficial flora	1197:1212	beneficial flora	1197:1212	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	53	theme	Firmicutes/Bacteroidetes	1002:1025	arg1	ratio					1027:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	4	54	theme	cytokine	777:784	arg1	levels					786:791	serum/tissue endotoxin and cytokine levels	750:791	levels	786:791	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30065236	0	55	theme	Antibiotic-Driven	76:92	arg1	Dysbiosis					105:113	Antibiotic-Driven Intestinal Dysbiosis	76:113	Antibiotic-Driven Intestinal Dysbiosis	76:113	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	3	56	theme	MiSeq	663:667	arg1	platform					669:676	the Illumina MiSeq platform	650:676	the Illumina MiSeq platform	650:676	Alteration and restoration in the microbial communities were elucidated by the Illumina MiSeq platform.
30065236	1	57	from	activity	212:219	arg1	indusiata					270:278	the mushroom Dictyophora indusiata	245:278	the mushroom Dictyophora indusiata	245:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	5	58	theme	taxonomic	976:984	arg1	levels					986:991	various taxonomic levels	968:991	various taxonomic levels	968:991	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	2	59	theme	function	516:523	arg1	recovery					454:461	the recovery	450:461	the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis	450:572	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	3	60	from	Alteration	575:584	arg1	communities					619:629	the microbial communities	605:629	the microbial communities	605:629	Alteration and restoration in the microbial communities were elucidated by the Illumina MiSeq platform.
30065236	5	61	theme	clindamycin	847:857	arg1	administration					829:842	Two-week daily oral administration	809:842	Two-week daily oral administration of clindamycin and metronidazole	809:875	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	62	theme	lactic	1243:1248	arg1	Lactobacillaceae					1225:1240	Lactobacillaceae	1225:1240	Lactobacillaceae (lactic acid-producing bacteria)	1225:1273	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	62	theme	lactic	1243:1248	arg1	bacteria					1265:1272	lactic acid-producing bacteria	1243:1272	lactic acid-producing bacteria	1243:1272	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	7	63	theme	microbiota	1786:1795	arg1	restoration					1767:1777	the restoration	1763:1777	the restoration of gut microbiota	1763:1795	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	2	64	theme	intestinal	497:506	arg1	function					516:523	intestinal barrier function	497:523	intestinal barrier function	497:523	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	3	65	from	restoration	590:600	arg1	communities					619:629	the microbial communities	605:629	the microbial communities	605:629	Alteration and restoration in the microbial communities were elucidated by the Illumina MiSeq platform.
30065236	5	66	theme	flora	1077:1081	arg1	ratio					1027:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	altered Firmicutes/Bacteroidetes ratio	994:1031	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	66	theme	flora	1077:1081	arg1	abundance					1056:1064	increased relative abundance	1037:1064	increased relative abundance	1037:1064	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	0	67	theme	Epithelial	132:141	arg1	Function					151:158	Gut Epithelial Barrier Function	128:158	Gut Epithelial Barrier Function in a Mouse Model	128:175	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	2	68	theme	broad-spectrum	531:544	arg1	dysbiosis					564:572	broad-spectrum antibiotic-driven dysbiosis	531:572	broad-spectrum antibiotic-driven dysbiosis	531:572	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	2	69	theme	indusiata	404:412	arg1	DIP					430:432	DIP	430:432	DIP	430:432	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	2	69	theme	indusiata	404:412	arg1	polysaccharide					414:427	D. indusiata polysaccharide	401:427	D. indusiata polysaccharide (DIP)	401:433	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	5	70	theme	bacterial	897:905	arg1	diversity					907:915	reduced bacterial diversity	889:915	reduced bacterial diversity	889:915	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	71	theme	relative	1047:1054	arg1	abundance					1056:1064	increased relative abundance	1037:1064	increased relative abundance	1037:1064	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	7	72	theme	DIP	1756:1758	arg1	effects					1743:1749	the protective effects	1728:1749	the protective effects of a DIP	1728:1758	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	5	73	theme	butyrate-producing	1296:1313	arg1	Ruminococaceae					1280:1293	Ruminococaceae (butyrate-producing bacteria)	1280:1323	Ruminococaceae (butyrate-producing bacteria)	1280:1323	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	73	theme	butyrate-producing	1296:1313	arg1	bacteria					1315:1322	butyrate-producing bacteria	1296:1322	butyrate-producing bacteria	1296:1322	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	5	74	theme	metronidazole	863:875	arg1	administration					829:842	Two-week daily oral administration	809:842	Two-week daily oral administration of clindamycin and metronidazole	809:875	Two-week daily oral administration of clindamycin and metronidazole resulted in reduced bacterial diversity and richness, and perturbed the microbial flora at various taxonomic levels (altered Firmicutes/Bacteroidetes ratio and increased relative abundance of harmful flora (Proteobacteria, Enterococcus, and Bacteroides)), whereas DIP administration reversed the dysbiosis and increased beneficial flora, including Lactobacillaceae (lactic acid-producing bacteria), and Ruminococaceae (butyrate-producing bacteria).
30065236	1	75	theme	microbiota	316:325	arg1	restoration					297:307	the restoration	293:307	the restoration of gut microbiota	293:325	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	7	76	theme	protective	1732:1741	arg1	effects					1743:1749	the protective effects	1728:1749	the protective effects of a DIP	1728:1758	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	2	77	theme	composition	481:491	arg1	recovery					454:461	the recovery	450:461	the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis	450:572	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	0	78	from	Function	151:158	arg1	Model					171:175	a Mouse Model	163:175	a Mouse Model	163:175	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	0	79	theme	Gut	128:130	arg1	Function					151:158	Gut Epithelial Barrier Function	128:158	Gut Epithelial Barrier Function in a Mouse Model	128:175	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	7	80	theme	endotoxin	1914:1922	arg1	levels					1924:1929	endotoxin levels	1914:1929	endotoxin levels	1914:1929	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	81	from	role	1822:1825	arg1	mice					1934:1937	mice	1934:1937	mice	1934:1937	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	81	from	role	1822:1825	arg1	lowering					1902:1909	lowering	1902:1909	lowering of endotoxin levels	1902:1929	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	81	from	role	1822:1825	arg1	enhancement					1834:1844	the enhancement	1830:1844	the enhancement of gut barrier integrity	1830:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	81	from	role	1822:1825	arg1	reduction					1872:1880	reduction	1872:1880	reduction of inflammation	1872:1896	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	2	82	theme	gut	466:468	arg1	composition					481:491	gut microbiota composition	466:491	gut microbiota composition	466:491	The present study aimed to investigate whether D. indusiata polysaccharide (DIP) could modulate the recovery of gut microbiota composition and intestinal barrier function after broad-spectrum antibiotic-driven dysbiosis.
30065236	6	83	theme	increased	1555:1563	arg1	expression					1565:1574	the increased expression	1551:1574	the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1)	1551:1658	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	7	84	theme	levels	1924:1929	arg1	lowering					1902:1909	lowering	1902:1909	lowering of endotoxin levels	1902:1929	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	84	theme	levels	1924:1929	arg1	enhancement					1834:1844	the enhancement	1830:1844	the enhancement of gut barrier integrity	1830:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	84	theme	levels	1924:1929	arg1	reduction					1872:1880	reduction	1872:1880	reduction of inflammation	1872:1896	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	6	85	theme	cytokine	1441:1448	arg1	levels					1538:1543	pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels	1424:1543	pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels	1424:1543	In addition, it resulted in the reduction of endotoxemia (through lipopolysaccharides (LPSs)) and pro-inflammatory cytokine (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)) levels, with the increased expression of tight-junction associated proteins (claudin-1, occludin, and zonula occludens-1).
30065236	1	86	theme	Dictyophora	258:268	arg1	indusiata					270:278	the mushroom Dictyophora indusiata	245:278	the mushroom Dictyophora indusiata	245:278	Despite the tremendous biological activity of polysaccharides from the mushroom Dictyophora indusiata, its role in the restoration of gut microbiota has not yet been explored.
30065236	7	87	theme	inflammation	1885:1896	arg1	lowering					1902:1909	lowering	1902:1909	lowering of endotoxin levels	1902:1929	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	87	theme	inflammation	1885:1896	arg1	enhancement					1834:1844	the enhancement	1830:1844	the enhancement of gut barrier integrity	1830:1869	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	7	87	theme	inflammation	1885:1896	arg1	reduction					1872:1880	reduction	1872:1880	reduction of inflammation	1872:1896	These findings not only suggested a comprehensive understanding of the protective effects of a DIP in the restoration of gut microbiota but also highlighted its role in the enhancement of gut barrier integrity, reduction of inflammation and lowering of endotoxin levels in mice.
30065236	0	88	theme	Barrier	143:149	arg1	Function					151:158	Gut Epithelial Barrier Function	128:158	Gut Epithelial Barrier Function in a Mouse Model	128:175	A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model.
30065236	4	89	theme	Colon	679:683	arg1	histology					685:693	Colon histology	679:693	Colon histology	679:693	Colon histology, expression of tight-junction associated proteins, and serum/tissue endotoxin and cytokine levels were evaluated.
30591361	7	0	theme	blood	1315:1319	arg1	serum					1321:1325	NFT spiked human blood serum	1298:1325	NFT spiked human blood serum	1298:1325	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	7	1	with	sample	1337:1342	arg1	performance					1369:1379	reliable analytical performance	1349:1379	reliable analytical performance	1349:1379	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	1	2	theme	β-cyclodextrin	193:206	arg1	synthesis					180:188	A simple ultrasonic energy assisted synthesis	144:188	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	144:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	7	3	dep	spiked	1302:1307	arg1	NFT					1298:1300	NFT	1298:1300	NFT	1298:1300	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	0	4	theme	electrochemical	101:115	arg1	sensing					117:123	electrochemical sensing	101:123	electrochemical sensing of nitrofurantoin	101:141	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	6	5	theme	good	1150:1153	arg1	selectivity					1155:1165	a good selectivity	1148:1165	a good selectivity	1148:1165	In addition, the sensor showcased a good selectivity, storage, and working stability, with amiable reproducibility.
30591361	0	6	theme	nitrofurantoin	128:141	arg1	sensing					117:123	electrochemical sensing	101:123	electrochemical sensing of nitrofurantoin	101:141	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	3	7	theme	β-CD/CNF	771:778	arg1	composite					780:788	the β-CD/CNF composite	767:788	the β-CD/CNF composite	767:788	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	8	8	theme	analytical	1458:1467	arg1	performance					1469:1479	outstanding analytical performance	1446:1479	outstanding analytical performance	1446:1479	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	5	9	theme	calculated	1062:1071	arg1	limit					1083:1087	calculated detection limit	1062:1087	calculated detection limit of 1.8 nM	1062:1097	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	1	10	theme	electrochemical	291:305	arg1	sensing					307:313	electrochemical sensing	291:313	electrochemical sensing of antibiotic nitrofurantoin (NFT)	291:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	2	11	theme	X-ray	582:586	arg1	spectroscopy					602:613	X-ray photoelectron spectroscopy	582:613	X-ray photoelectron spectroscopy	582:613	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	7	12	with	serum	1321:1325	arg1	performance					1369:1379	reliable analytical performance	1349:1379	reliable analytical performance	1349:1379	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	4	13	theme	composite	846:854	arg1	efficacy					821:828	the electrocatalytic efficacy	800:828	the electrocatalytic efficacy of the β-CD/CNF composite	800:854	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	2	14	theme	surface	393:399	arg1	morphology					401:410	surface morphology	393:410	surface morphology	393:410	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	5	15	theme	working	1028:1034	arg1	range					1036:1040	a broad working range	1020:1040	a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively	1020:1111	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	6	16	theme	working	1181:1187	arg1	stability					1189:1197	working stability	1181:1197	working stability	1181:1197	In addition, the sensor showcased a good selectivity, storage, and working stability, with amiable reproducibility.
30591361	5	17	theme	limit	1083:1087	arg1	range					1036:1040	a broad working range	1020:1040	a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively	1020:1111	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	5	18	theme	broad	1022:1026	arg1	range					1036:1040	a broad working range	1020:1040	a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively	1020:1111	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	2	19	theme	energy	505:510	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	19	theme	energy	505:510	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	8	20	theme	real-world	1484:1493	arg1	samples					1495:1501	real-world samples	1484:1501	real-world samples with higher reliability	1484:1525	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	4	21	theme	quantitative	930:941	arg1	detection					943:951	the high sensitive quantitative detection	911:951	the high sensitive quantitative detection of NFT	911:958	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	7	22	theme	spiked	1302:1307	arg1	serum					1321:1325	NFT spiked human blood serum	1298:1325	NFT spiked human blood serum	1298:1325	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	2	23	theme	composite	428:436	arg1	composition					377:387	elemental composition	367:387	elemental composition	367:387	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	23	theme	composite	428:436	arg1	morphology					401:410	surface morphology	393:410	surface morphology	393:410	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	0	24	theme	energy-assisted	11:25	arg1	preparation					27:37	Ultrasonic energy-assisted preparation	0:37	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.	0:142	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	4	25	theme	NFT	956:958	arg1	detection					943:951	the high sensitive quantitative detection	911:951	the high sensitive quantitative detection of NFT	911:958	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	5	26	theme	0.004-308 µM	1045:1056	arg1	range					1036:1040	a broad working range	1020:1040	a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively	1020:1111	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	2	27	theme	β-CD/CNF	419:426	arg1	composite					428:436	the β-CD/CNF composite	415:436	the β-CD/CNF composite	415:436	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	0	28	theme	Ultrasonic	0:9	arg1	preparation					27:37	Ultrasonic energy-assisted preparation	0:37	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.	0:142	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	3	29	theme	uniform	652:658	arg1	enfolding					660:668	The uniform enfolding	648:668	The uniform enfolding of hydrophilic β-CD over CNF	648:697	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	1	30	theme	carbon	225:230	arg1	composite					242:250	carbon nanofiber composite	225:250	carbon nanofiber composite (CNF)	225:256	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	1	30	theme	carbon	225:230	arg1	CNF					253:255	CNF	253:255	CNF	253:255	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	2	31	theme	electron	484:491	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	31	theme	electron	484:491	arg1	Fourier					541:547	Fourier	541:547	Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	541:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	31	theme	electron	484:491	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	1	32	theme	antibiotic	318:327	arg1	nitrofurantoin					329:342	antibiotic nitrofurantoin	318:342	antibiotic nitrofurantoin (NFT)	318:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	1	32	theme	antibiotic	318:327	arg1	NFT					345:347	NFT	345:347	NFT	345:347	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	2	33	theme	thermogravimetric	620:636	arg1	analysis					638:645	thermogravimetric analysis	620:645	thermogravimetric analysis	620:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	34	dep	composition	377:387	arg1	The					363:365	The	363:365	The	363:365	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	1	35	theme	nanofiber	232:240	arg1	composite					242:250	carbon nanofiber composite	225:250	carbon nanofiber composite (CNF)	225:256	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	1	35	theme	nanofiber	232:240	arg1	CNF					253:255	CNF	253:255	CNF	253:255	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	2	36	theme	scanning	475:482	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	36	theme	scanning	475:482	arg1	Fourier					541:547	Fourier	541:547	Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	541:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	36	theme	scanning	475:482	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	1	37	theme	nitrofurantoin	329:342	arg1	sensing					307:313	electrochemical sensing	291:313	electrochemical sensing of antibiotic nitrofurantoin (NFT)	291:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	2	38	theme	Field	460:464	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	38	theme	Field	460:464	arg1	Fourier					541:547	Fourier	541:547	Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	541:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	38	theme	Field	460:464	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	0	39	theme	nanofiber	64:72	arg1	composite					74:82	β-cyclodextrin-carbon nanofiber composite	42:82	β-cyclodextrin-carbon nanofiber composite	42:82	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	2	40	theme	elemental	367:375	arg1	composition					377:387	elemental composition	367:387	elemental composition	367:387	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	1	41	from	application	276:286	arg1	sensing					307:313	electrochemical sensing	291:313	electrochemical sensing of antibiotic nitrofurantoin (NFT)	291:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	8	42	theme	simple	1386:1391	arg1	sensor					1413:1418	The simple, cost-effective NFT sensor	1382:1418	The simple, cost-effective NFT sensor based on β-CD/CNF	1382:1436	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	3	43	theme	hydrophilic	673:683	arg1	β-CD					685:688	hydrophilic β-CD	673:688	hydrophilic β-CD over CNF	673:697	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	5	44	theme	analytical	977:986	arg1	conditions					988:997	optimized analytical conditions	967:997	optimized analytical conditions	967:997	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	5	45	theme	optimized	967:975	arg1	conditions					988:997	optimized analytical conditions	967:997	optimized analytical conditions	967:997	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	0	46	theme	β-cyclodextrin-carbon	42:62	arg1	composite					74:82	β-cyclodextrin-carbon nanofiber composite	42:82	β-cyclodextrin-carbon nanofiber composite	42:82	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	2	47	theme	X-ray	523:527	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	47	theme	X-ray	523:527	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	3	48	theme	aqueous	711:717	arg1	dispersion					719:728	the aqueous dispersion	707:728	the aqueous dispersion	707:728	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	3	49	theme	β-CD	685:688	arg1	enfolding					660:668	The uniform enfolding	648:668	The uniform enfolding of hydrophilic β-CD over CNF	648:697	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	8	50	with	samples	1495:1501	arg1	reliability					1515:1525	higher reliability	1508:1525	higher reliability	1508:1525	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	8	51	theme	NFT	1409:1411	arg1	sensor					1413:1418	The simple, cost-effective NFT sensor	1382:1418	The simple, cost-effective NFT sensor based on β-CD/CNF	1382:1436	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	2	52	theme	dispersive	512:521	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	52	theme	dispersive	512:521	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	53	theme	photoelectron	588:600	arg1	spectroscopy					602:613	X-ray photoelectron spectroscopy	582:613	X-ray photoelectron spectroscopy	582:613	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	54	dep	Fourier	541:547	arg1	transform					549:557	transform	549:557	transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	549:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	4	55	theme	sensitive	920:928	arg1	detection					943:951	the high sensitive quantitative detection	911:951	the high sensitive quantitative detection of NFT	911:958	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	6	56	theme	amiable	1205:1211	arg1	reproducibility					1213:1227	amiable reproducibility	1205:1227	amiable reproducibility	1205:1227	In addition, the sensor showcased a good selectivity, storage, and working stability, with amiable reproducibility.
30591361	1	57	theme	simple	146:151	arg1	synthesis					180:188	A simple ultrasonic energy assisted synthesis	144:188	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	144:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	0	58	theme	composite	74:82	arg1	preparation					27:37	Ultrasonic energy-assisted preparation	0:37	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.	0:142	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	4	59	theme	high	915:918	arg1	detection					943:951	the high sensitive quantitative detection	911:951	the high sensitive quantitative detection of NFT	911:958	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	7	60	theme	human	1309:1313	arg1	serum					1321:1325	NFT spiked human blood serum	1298:1325	NFT spiked human blood serum	1298:1325	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	1	61	theme	ultrasonic	153:162	arg1	synthesis					180:188	A simple ultrasonic energy assisted synthesis	144:188	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	144:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	4	62	used	utilized	859:866	arg2	efficacy					821:828	the electrocatalytic efficacy	800:828	the electrocatalytic efficacy of the β-CD/CNF composite	800:854	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	7	63	theme	analytical	1358:1367	arg1	performance					1369:1379	reliable analytical performance	1349:1379	reliable analytical performance	1349:1379	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	7	64	theme	point-of-care	1234:1246	arg1	applicability					1248:1260	The point-of-care applicability	1230:1260	The point-of-care applicability of the sensor	1230:1274	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	5	65	theme	1.8 nM	1092:1097	arg1	0.004-308 µM					1045:1056	0.004-308 µM	1045:1056	0.004-308 µM	1045:1056	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	5	65	theme	1.8 nM	1092:1097	arg1	limit					1083:1087	calculated detection limit	1062:1087	calculated detection limit of 1.8 nM	1062:1097	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	1	66	from	composite	242:250	arg1	sensing					307:313	electrochemical sensing	291:313	electrochemical sensing of antibiotic nitrofurantoin (NFT)	291:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	1	67	theme	energy	164:169	arg1	synthesis					180:188	A simple ultrasonic energy assisted synthesis	144:188	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	144:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	4	68	theme	β-CD/CNF	837:844	arg1	composite					846:854	the β-CD/CNF composite	833:854	the β-CD/CNF composite	833:854	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	2	69	dep	transform	549:557	arg1	infrared					559:566	infrared	559:566	transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	549:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	3	70	theme	abundant	740:747	arg1	surface					756:762	abundant active surface	740:762	abundant active surface	740:762	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	0	71	dep	preparation	27:37	arg1	Application					85:95	Application	85:95	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.	0:142	Ultrasonic energy-assisted preparation of β-cyclodextrin-carbon nanofiber composite: Application for electrochemical sensing of nitrofurantoin.
30591361	8	72	dep	simple	1386:1391	arg1	cost-effective					1394:1407	cost-effective	1394:1407	cost-effective	1394:1407	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	1	73	theme	assisted	171:178	arg1	synthesis					180:188	A simple ultrasonic energy assisted synthesis	144:188	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	144:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
30591361	8	74	theme	outstanding	1446:1456	arg1	performance					1469:1479	outstanding analytical performance	1446:1479	outstanding analytical performance	1446:1479	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	4	75	theme	electrocatalytic	804:819	arg1	efficacy					821:828	the electrocatalytic efficacy	800:828	the electrocatalytic efficacy of the β-CD/CNF composite	800:854	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	3	76	theme	active	749:754	arg1	surface					756:762	abundant active surface	740:762	abundant active surface	740:762	The uniform enfolding of hydrophilic β-CD over CNF enhance the aqueous dispersion and offer abundant active surface to the β-CD/CNF composite.
30591361	2	77	theme	emission	466:473	arg1	microscopy					493:502	Field emission scanning electron microscopy	460:502	Field emission scanning electron microscopy	460:502	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	77	theme	emission	466:473	arg1	Fourier					541:547	Fourier	541:547	Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis	541:645	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	2	77	theme	emission	466:473	arg1	microscopy					529:538	energy dispersive X-ray microscopy	505:538	energy dispersive X-ray microscopy	505:538	The elemental composition and surface morphology of the β-CD/CNF composite was validated through Field emission scanning electron microscopy, energy dispersive X-ray microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis.
30591361	7	78	theme	reliable	1349:1356	arg1	performance					1369:1379	reliable analytical performance	1349:1379	reliable analytical performance	1349:1379	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	8	79	theme	higher	1508:1513	arg1	reliability					1515:1525	higher reliability	1508:1525	higher reliability	1508:1525	The simple, cost-effective NFT sensor based on β-CD/CNF offered outstanding analytical performance in real-world samples with higher reliability.
30591361	7	80	theme	urine	1331:1335	arg1	sample					1337:1342	urine sample	1331:1342	urine sample with reliable analytical performance	1331:1379	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	5	81	theme	detection	1073:1081	arg1	limit					1083:1087	calculated detection limit	1062:1087	calculated detection limit of 1.8 nM	1062:1097	Under optimized analytical conditions, the sensor displays a broad working range of 0.004-308 µM and calculated detection limit of 1.8 nM, respectively.
30591361	4	82	theme	electrochemical	884:898	arg1	sensor					900:905	an electrochemical sensor	881:905	an electrochemical sensor for the high sensitive quantitative detection of NFT	881:958	Further, the electrocatalytic efficacy of the β-CD/CNF composite is utilized to fabricate an electrochemical sensor for the high sensitive quantitative detection of NFT.
30591361	7	83	theme	sensor	1269:1274	arg1	applicability					1248:1260	The point-of-care applicability	1230:1260	The point-of-care applicability of the sensor	1230:1274	The point-of-care applicability of the sensor was demonstrated with NFT spiked human blood serum and urine sample with reliable analytical performance.
30591361	1	84	theme	potential	266:274	arg1	application					276:286	its potential application	262:286	its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT)	262:348	A simple ultrasonic energy assisted synthesis of β-cyclodextrin (β-CD) supported carbon nanofiber composite (CNF) and its potential application in electrochemical sensing of antibiotic nitrofurantoin (NFT) is reported.
31655479	0	0	theme	quaternary	87:96	arg1	ammonium					98:105	novel quaternary ammonium	81:105	novel quaternary ammonium chitosan composite	81:124	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	1	1	theme	food	159:162	arg1	coating					184:190	a multifunctional food packaging composite coating	141:190	a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties	141:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	3	2	dep	ammonium	704:711	arg1	chitosan					713:720	chitosan	713:720	chitosan	713:720	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	3	2	dep	ammonium	704:711	arg1	PVA					726:728	PVA	726:728	PVA	726:728	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	0	3	theme	novel	81:85	arg1	ammonium					98:105	novel quaternary ammonium	81:105	novel quaternary ammonium chitosan composite	81:124	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	3	4	theme	ammonium	704:711	arg1	absorbency					679:688	the strong water absorbency	662:688	the strong water absorbency of quaternary ammonium chitosan and PVA	662:728	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	7	5	contain	has	1132:1134	arg1	coating					1124:1130	The multifunctional coating	1104:1130	The multifunctional coating	1104:1130	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	7	5	contain	has	1132:1134	arg2	prospects					1142:1150	broad prospects	1136:1150	broad prospects	1136:1150	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	1	6	theme	packaging	164:172	arg1	coating					184:190	a multifunctional food packaging composite coating	141:190	a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties	141:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	2	7	theme	excellent	524:532	arg1	functions					564:572	excellent antifogging and antibacterial functions	524:572	excellent antifogging and antibacterial functions	524:572	A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions.
31655479	2	8	theme	antibacterial	550:562	arg1	functions					564:572	excellent antifogging and antibacterial functions	524:572	excellent antifogging and antibacterial functions	524:572	A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions.
31655479	1	9	theme	vinyl	357:361	arg1	poly					351:354	poly	351:354	poly (vinyl alcohol) (PVA)	351:376	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	9	theme	vinyl	357:361	arg1	alcohol					363:369	vinyl alcohol	357:369	vinyl alcohol	357:369	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	4	10	theme	antifogging	802:812	arg1	test					814:817	antifogging test	802:817	antifogging test	802:817	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	5	11	theme	Botrytis	966:973	arg1	cinerea					975:981	Botrytis cinerea	966:981	Botrytis cinerea	966:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	7	12	theme	broad	1136:1140	arg1	prospects					1142:1150	broad prospects	1136:1150	broad prospects	1136:1150	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	3	13	theme	excellent	579:587	arg1	property					601:608	The excellent antifogging property	575:608	The excellent antifogging property of the HACC/PVA composite coating	575:642	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	2	14	theme	antifogging	534:544	arg1	functions					564:572	excellent antifogging and antibacterial functions	524:572	excellent antifogging and antibacterial functions	524:572	A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions.
31655479	5	15	theme	composite	869:877	arg1	coating					879:885	the HACC/PVA composite coating	856:885	the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea	856:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	5	16	theme	inhibition	837:846	arg1	rate					848:851	the inhibition rate	833:851	the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea	833:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	5	16	theme	inhibition	837:846	arg1	%					997:997	up to ~99%	988:997	up to ~99%	988:997	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	1	17	theme	composite	174:182	arg1	coating					184:190	a multifunctional food packaging composite coating	141:190	a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties	141:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	0	18	theme	composite	116:124	arg1	ammonium					98:105	novel quaternary ammonium	81:105	novel quaternary ammonium chitosan composite	81:124	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	5	19	dep	aureus	943:948	arg1	aureus					954:959	S. aureus	951:959	S. aureus	951:959	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	2	20	theme	simple	443:448	arg1	modification					465:476	A simple quaternization modification	441:476	A simple quaternization modification	441:476	A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions.
31655479	7	21	theme	fruit	1174:1178	arg1	application					1159:1169	the application	1155:1169	the application of fruit and vegetable packaging	1155:1202	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	0	22	theme	efficient	7:15	arg1	antifogging					17:27	Highly efficient antifogging	0:27	Highly efficient antifogging	0:27	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	4	23	theme	glasses	774:780	arg1	ratio					758:762	A nearly 98% transmittance ratio	731:762	A nearly 98% transmittance ratio of coated glasses	731:780	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	5	24	from	%	997:997	arg1	addition					823:830	addition	823:830	addition	823:830	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	1	25	theme	quaternary	297:306	arg1	HACC					341:344	HACC	341:344	HACC	341:344	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	25	theme	quaternary	297:306	arg1	chitosan					331:338	quaternary ammonium salt modified chitosan	297:338	quaternary ammonium salt modified chitosan (HACC)	297:345	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	4	26	theme	coated	767:772	arg1	glasses					774:780	coated glasses	767:780	coated glasses	767:780	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	1	27	theme	transparent	197:207	arg1	properties					255:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	28	theme	ammonium	308:315	arg1	HACC					341:344	HACC	341:344	HACC	341:344	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	28	theme	ammonium	308:315	arg1	chitosan					331:338	quaternary ammonium salt modified chitosan	297:338	quaternary ammonium salt modified chitosan (HACC)	297:345	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	3	29	theme	antifogging	589:599	arg1	property					601:608	The excellent antifogging property	575:608	The excellent antifogging property of the HACC/PVA composite coating	575:642	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	5	30	dep	coli	912:915	arg1	coli					921:924	E. coli	918:924	E. coli	918:924	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	5	31	theme	HACC/PVA	860:867	arg1	coating					879:885	the HACC/PVA composite coating	856:885	the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea	856:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	4	32	dep	ratio	758:762	arg1	%					742:742	%	742:742	%	742:742	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	1	33	theme	salt	317:320	arg1	HACC					341:344	HACC	341:344	HACC	341:344	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	33	theme	salt	317:320	arg1	chitosan					331:338	quaternary ammonium salt modified chitosan	297:338	quaternary ammonium salt modified chitosan (HACC)	297:345	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	4	34	dep	%	742:742	arg1	98					740:741	98	740:741	98	740:741	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	1	35	theme	biodegradable	210:222	arg1	properties					255:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	0	36	theme	food	47:50	arg1	film					62:65	antibacterial food packaging film	33:65	antibacterial food packaging film	33:65	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	1	37	theme	modified	322:329	arg1	HACC					341:344	HACC	341:344	HACC	341:344	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	37	theme	modified	322:329	arg1	chitosan					331:338	quaternary ammonium salt modified chitosan	297:338	quaternary ammonium salt modified chitosan (HACC)	297:345	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	3	38	theme	quaternary	693:702	arg1	ammonium					704:711	quaternary ammonium chitosan and PVA	693:728	quaternary ammonium chitosan and PVA	693:728	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	3	39	theme	coating	636:642	arg1	property					601:608	The excellent antifogging property	575:608	The excellent antifogging property of the HACC/PVA composite coating	575:642	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	6	40	theme	strawberries	1059:1070	arg1	group					1050:1054	each group	1045:1054	each group of strawberries	1045:1070	The antibacterial effect was demonstrated by each group of strawberries after storage for 1, 3, 5 days.
31655479	6	40	theme	strawberries	1059:1070	arg1	strawberries					1059:1070	strawberries	1059:1070	strawberries	1059:1070	The antibacterial effect was demonstrated by each group of strawberries after storage for 1, 3, 5 days.
31655479	5	41	theme	coating	879:885	arg1	rate					848:851	the inhibition rate	833:851	the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea	833:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	5	41	theme	coating	879:885	arg1	%					997:997	up to ~99%	988:997	up to ~99%	988:997	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	0	42	theme	antibacterial	33:45	arg1	film					62:65	antibacterial food packaging film	33:65	antibacterial food packaging film	33:65	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	3	43	theme	water	673:677	arg1	absorbency					679:688	the strong water absorbency	662:688	the strong water absorbency of quaternary ammonium chitosan and PVA	662:728	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	1	44	theme	antifogging	225:235	arg1	properties					255:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	7	45	theme	multifunctional	1108:1122	arg1	coating					1124:1130	The multifunctional coating	1104:1130	The multifunctional coating	1104:1130	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	7	46	theme	vegetable	1184:1192	arg1	application					1159:1169	the application	1155:1169	the application of fruit and vegetable packaging	1155:1202	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	7	47	dep	fruit	1174:1178	arg1	packaging					1194:1202	packaging	1194:1202	packaging	1194:1202	The multifunctional coating has broad prospects in the application of fruit and vegetable packaging.
31655479	1	48	theme	facile	384:389	arg1	method					433:438	a facile and environment-friendly solution casting method	382:438	a facile and environment-friendly solution casting method	382:438	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	3	49	theme	HACC/PVA	617:624	arg1	coating					636:642	the HACC/PVA composite coating	613:642	the HACC/PVA composite coating	613:642	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	4	50	theme	transmittance	744:756	arg1	ratio					758:762	A nearly 98% transmittance ratio	731:762	A nearly 98% transmittance ratio of coated glasses	731:780	A nearly 98% transmittance ratio of coated glasses was achieved during antifogging test.
31655479	0	51	theme	packaging	52:60	arg1	film					62:65	antibacterial food packaging film	33:65	antibacterial food packaging film	33:65	Highly efficient antifogging and antibacterial food packaging film fabricated by novel quaternary ammonium chitosan composite.
31655479	2	52	theme	quaternization	450:463	arg1	modification					465:476	A simple quaternization modification	441:476	A simple quaternization modification	441:476	A simple quaternization modification enabled the coating simultaneously to achieve excellent antifogging and antibacterial functions.
31655479	3	53	theme	composite	626:634	arg1	coating					636:642	the HACC/PVA composite coating	613:642	the HACC/PVA composite coating	613:642	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	1	54	theme	environment-friendly	395:414	arg1	method					433:438	a facile and environment-friendly solution casting method	382:438	a facile and environment-friendly solution casting method	382:438	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	5	55	dep	coating	879:885	arg1	kill					887:890	kill	887:890	kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea	887:981	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	1	56	with	coating	184:190	arg1	properties					255:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	57	theme	solution	416:423	arg1	method					433:438	a facile and environment-friendly solution casting method	382:438	a facile and environment-friendly solution casting method	382:438	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	3	58	theme	strong	666:671	arg1	absorbency					679:688	the strong water absorbency	662:688	the strong water absorbency of quaternary ammonium chitosan and PVA	662:728	The excellent antifogging property of the HACC/PVA composite coating was attributed to the strong water absorbency of quaternary ammonium chitosan and PVA.
31655479	1	59	theme	antibacterial	241:253	arg1	properties					255:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	transparent, biodegradable, antifogging and antibacterial properties	197:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	1	60	theme	casting	425:431	arg1	method					433:438	a facile and environment-friendly solution casting method	382:438	a facile and environment-friendly solution casting method	382:438	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	5	61	dep	~99	994:996	arg1	to					991:992	to	991:992	to	991:992	In addition, the inhibition rate of the HACC/PVA composite coating kill against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Botrytis cinerea were up to ~99%.
31655479	1	62	theme	multifunctional	143:157	arg1	coating					184:190	a multifunctional food packaging composite coating	141:190	a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties	141:264	In this work, a multifunctional food packaging composite coating with transparent, biodegradable, antifogging and antibacterial properties was designed and fabricated by quaternary ammonium salt modified chitosan (HACC) and poly (vinyl alcohol) (PVA) via a facile and environment-friendly solution casting method.
31655479	6	63	theme	antibacterial	1004:1016	arg1	effect					1018:1023	The antibacterial effect	1000:1023	The antibacterial effect	1000:1023	The antibacterial effect was demonstrated by each group of strawberries after storage for 1, 3, 5 days.
31079689	0	0	theme	prebiotic	91:99	arg1	potentials					119:128	prebiotic and antiglycation potentials	91:128	prebiotic and antiglycation potentials	91:128	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	6	1	theme	prebiotic	1175:1183	arg1	activity					1185:1192	both antiglycation and prebiotic activity	1152:1192	both antiglycation and prebiotic activity of POSs	1152:1200	The degree of esterification (DE) was less important for both antiglycation and prebiotic activity of POSs.
31079689	1	2	theme	prebiotic	167:175	arg1	activities					195:204	prebiotic and antiglycation activities	167:204	prebiotic and antiglycation activities	167:204	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	2	3	theme	polysaccharide	380:393	arg1	degradation					358:368	enzymatic and ultrasound-assisted enzymatic degradation	314:368	degradation	358:368	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	4	4	theme	antiglycation	611:623	arg1	activity					625:632	the antiglycation activity	607:632	the antiglycation activity of POSs in vitro	607:649	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	4	theme	antiglycation	611:623	arg1	proportional					655:666	proportional	655:666	proportional	655:666	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	0	5	theme	antiglycation	105:117	arg1	potentials					119:128	prebiotic and antiglycation potentials	91:128	prebiotic and antiglycation potentials	91:128	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	4	6	theme	molar	859:863	arg1	ratios					865:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	5	7	theme	prebiotic	913:921	arg1	activity					923:930	the prebiotic activity	909:930	the prebiotic activity of POSs	909:938	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	4	8	theme	POSs	637:640	arg1	activity					625:632	the antiglycation activity	607:632	the antiglycation activity of POSs in vitro	607:649	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	8	theme	POSs	637:640	arg1	proportional					655:666	proportional	655:666	proportional	655:666	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	3	9	theme	POSs	511:514	arg1	activities					497:506	the antiglycation in vitro and prebiotic activities	456:506	the antiglycation in vitro and prebiotic activities of POSs	456:514	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	4	10	from	activity	625:632	arg1	vitro					645:649	vitro	645:649	vitro	645:649	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	1	11	theme	Pectin	131:136	arg1	POSs					156:159	POSs	156:159	POSs	156:159	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	1	11	theme	Pectin	131:136	arg1	oligosaccharides					138:153	Pectin oligosaccharides	131:153	Pectin oligosaccharides (POSs)	131:160	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	6	12	theme	POSs	1197:1200	arg1	activity					1185:1192	both antiglycation and prebiotic activity	1152:1192	both antiglycation and prebiotic activity of POSs	1152:1200	The degree of esterification (DE) was less important for both antiglycation and prebiotic activity of POSs.
31079689	5	13	dep	Ara	971:973	arg1	Rha					975:977	Rha	975:977	Rha	975:977	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	13	dep	Ara	971:973	arg1	Rha					987:989	Rha	987:989	Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content	971:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	13	dep	Ara	971:973	arg1	Gal					983:985	Gal	983:985	Gal	983:985	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	14	theme	POSs	935:938	arg1	activity					923:930	the prebiotic activity	909:930	the prebiotic activity of POSs	909:938	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	1	15	theme	antiglycation	181:193	arg1	activities					195:204	prebiotic and antiglycation activities	167:204	prebiotic and antiglycation activities	167:204	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	0	16	theme	potentials	119:128	arg1	relationship					75:86	Structure-activity relationship	56:86	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.	0:129	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	1	17	contain	have	162:165	arg1	POSs					156:159	POSs	156:159	POSs	156:159	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	1	17	contain	have	162:165	arg1	oligosaccharides					138:153	Pectin oligosaccharides	131:153	Pectin oligosaccharides (POSs)	131:160	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	1	17	contain	have	162:165	arg2	activities					195:204	prebiotic and antiglycation activities	167:204	prebiotic and antiglycation activities	167:204	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	3	18	theme	chemical	429:436	arg1	characterization					438:453	the chemical characterization	425:453	the chemical characterization	425:453	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	3	19	theme	in	474:475	arg1	activities					497:506	the antiglycation in vitro and prebiotic activities	456:506	the antiglycation in vitro and prebiotic activities of POSs	456:514	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	2	20	theme	ultrasound-assisted	328:346	arg1	degradation					358:368	enzymatic and ultrasound-assisted enzymatic degradation	314:368	degradation	358:368	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	5	21	theme	neutral	1036:1042	arg1	content					1050:1056	the neutral sugar content	1032:1056	the neutral sugar content	1032:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	0	22	theme	Pectin	0:5	arg1	oligosaccharides					7:22	Pectin oligosaccharides	0:22	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.	0:129	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	4	23	theme	low	742:744	arg1	weight					756:761	the low molecular weight	738:761	the low molecular weight POSs	738:766	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	24	theme	weight	756:761	arg1	POSs					763:766	the low molecular weight POSs	738:766	the low molecular weight POSs	738:766	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	25	dep	proportional	793:804	arg1	such					831:834	such	831:834	such	831:834	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	26	theme	branching	813:821	arg1	degree					823:828	its branching degree	809:828	its branching degree	809:828	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	2	27	theme	enzymatic	314:322	arg1	degradation					358:368	enzymatic and ultrasound-assisted enzymatic degradation	314:368	degradation	358:368	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	3	28	theme	prebiotic	487:495	arg1	activities					497:506	the antiglycation in vitro and prebiotic activities	456:506	the antiglycation in vitro and prebiotic activities of POSs	456:514	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	4	29	dep	Ara	839:841	arg1	Rha					843:845	Rha	843:845	Rha	843:845	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	29	dep	Ara	839:841	arg1	Rha					855:857	Rha	855:857	Ara:Rha and Gal:Rha	839:857	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	29	dep	Ara	839:841	arg1	Gal					851:853	Gal	851:853	Gal	851:853	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	6	30	theme	esterification	1109:1122	arg1	important					1138:1146	important	1138:1146	important	1138:1146	The degree of esterification (DE) was less important for both antiglycation and prebiotic activity of POSs.
31079689	6	30	theme	esterification	1109:1122	arg1	degree					1099:1104	The degree	1095:1104	The degree of esterification (DE)	1095:1127	The degree of esterification (DE) was less important for both antiglycation and prebiotic activity of POSs.
31079689	5	31	theme	sugar	1044:1048	arg1	content					1050:1056	the neutral sugar content	1032:1056	the neutral sugar content	1032:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	4	32	theme	molar	714:718	arg1	ratios					720:725	Rha molar ratios	710:725	Rha molar ratios	710:725	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	32	theme	molar	714:718	arg1	LM-POSs					769:775	LM-POSs	769:775	LM-POSs	769:775	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	0	33	theme	Actinidia	38:46	arg1	arguta					48:53	Actinidia arguta	38:53	Actinidia arguta	38:53	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	7	34	theme	important	1229:1237	arg1	basis					1251:1255	an important theoretical basis	1226:1255	an important theoretical basis for POSs application in food	1226:1284	These results provided an important theoretical basis for POSs application in food.
31079689	5	35	theme	molecule	1007:1014	arg1	composition					1016:1026	molecule composition	1007:1026	molecule composition	1007:1026	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	7	36	theme	POSs	1261:1264	arg1	application					1266:1276	POSs application	1261:1276	POSs application in food	1261:1284	These results provided an important theoretical basis for POSs application in food.
31079689	4	37	theme	acid	688:691	arg1	content					693:699	the galacturonic acid content	671:699	the galacturonic acid content	671:699	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	3	38	theme	antiglycation	460:472	arg1	activities					497:506	the antiglycation in vitro and prebiotic activities	456:506	the antiglycation in vitro and prebiotic activities of POSs	456:514	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	6	39	theme	antiglycation	1157:1169	arg1	activity					1185:1192	both antiglycation and prebiotic activity	1152:1192	both antiglycation and prebiotic activity of POSs	1152:1200	The degree of esterification (DE) was less important for both antiglycation and prebiotic activity of POSs.
31079689	1	40	theme	specific	224:231	arg1	unclear					268:274	unclear	268:274	unclear	268:274	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	1	40	theme	specific	224:231	arg1	relationship					252:263	the specific structure-activity relationship	220:263	the specific structure-activity relationship	220:263	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	5	41	located	found	898:902	arg2	it					886:887	it	886:887	it	886:887	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	41	located	found	898:902	arg1	addition					876:883	addition	876:883	addition	876:883	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	42	from	ratios	997:1002	arg1	content					1050:1056	the neutral sugar content	1032:1056	the neutral sugar content	1032:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	42	from	ratios	997:1002	arg1	composition					1016:1026	molecule composition	1007:1026	molecule composition	1007:1026	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	4	43	dep	showed	595:600	arg1	ratios					720:725	Rha molar ratios	710:725	Rha molar ratios	710:725	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	43	dep	showed	595:600	arg1	ratios					865:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	43	dep	showed	595:600	arg1	LM-POSs					769:775	LM-POSs	769:775	LM-POSs	769:775	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	2	44	theme	pectin	373:378	arg1	PPS					396:398	PPS	396:398	PPS	396:398	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	2	44	theme	pectin	373:378	arg1	polysaccharide					380:393	pectin polysaccharide	373:393	pectin polysaccharide (PPS)	373:399	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	0	45	theme	arguta	48:53	arg1	fruit					29:33	fruit	29:33	fruit of Actinidia arguta	29:53	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	3	46	dep	in	474:475	arg1	vitro					477:481	vitro	477:481	vitro	477:481	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	4	47	theme	molecular	746:754	arg1	weight					756:761	the low molecular weight	738:761	the low molecular weight POSs	738:766	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	1	48	theme	structure-activity	233:250	arg1	unclear					268:274	unclear	268:274	unclear	268:274	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	1	48	theme	structure-activity	233:250	arg1	relationship					252:263	the specific structure-activity relationship	220:263	the specific structure-activity relationship	220:263	Pectin oligosaccharides (POSs) have prebiotic and antiglycation activities in vitro, but the specific structure-activity relationship is unclear.
31079689	5	49	theme	molar	991:995	arg1	arabinose					1084:1092	arabinose	1084:1092	arabinose	1084:1092	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	49	theme	molar	991:995	arg1	ratios					997:1002	Ara:Rha and Gal:Rha molar ratios	971:1002	Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content	971:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	49	theme	molar	991:995	arg1	galactose					1070:1078	galactose	1070:1078	galactose	1070:1078	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	4	50	theme	proportional	793:804	arg1	ratios					865:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios	783:870	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	2	51	theme	enzymatic	348:356	arg1	degradation					358:368	enzymatic and ultrasound-assisted enzymatic degradation	314:368	degradation	358:368	In this study, POSs were obtained by enzymatic and ultrasound-assisted enzymatic degradation of pectin polysaccharide (PPS), respectively.
31079689	4	52	theme	galacturonic	675:686	arg1	content					693:699	the galacturonic acid content	671:699	the galacturonic acid content	671:699	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	0	53	dep	oligosaccharides	7:22	arg1	relationship					75:86	Structure-activity relationship	56:86	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.	0:129	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	7	54	theme	theoretical	1239:1249	arg1	basis					1251:1255	an important theoretical basis	1226:1255	an important theoretical basis for POSs application in food	1226:1284	These results provided an important theoretical basis for POSs application in food.
31079689	3	55	theme	structure-activity	538:555	arg1	relationship					557:568	the structure-activity relationship	534:568	the structure-activity relationship	534:568	Based on the chemical characterization, the antiglycation in vitro and prebiotic activities of POSs were compared and the structure-activity relationship was studied.
31079689	0	56	theme	Structure-activity	56:73	arg1	relationship					75:86	Structure-activity relationship	56:86	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.	0:129	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	0	57	from	fruit	29:33	arg1	oligosaccharides					7:22	Pectin oligosaccharides	0:22	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.	0:129	Pectin oligosaccharides from fruit of Actinidia arguta: Structure-activity relationship of prebiotic and antiglycation potentials.
31079689	5	58	theme	Ara	971:973	arg1	arabinose					1084:1092	arabinose	1084:1092	arabinose	1084:1092	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	58	theme	Ara	971:973	arg1	ratios					997:1002	Ara:Rha and Gal:Rha molar ratios	971:1002	Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content	971:1056	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	5	58	theme	Ara	971:973	arg1	galactose					1070:1078	galactose	1070:1078	galactose	1070:1078	In addition, it was also found that the prebiotic activity of POSs was positively correlated with Ara:Rha and Gal:Rha molar ratios in molecule composition and the neutral sugar content, especially galactose and arabinose.
31079689	7	59	from	application	1266:1276	arg1	food					1281:1284	food	1281:1284	food	1281:1284	These results provided an important theoretical basis for POSs application in food.
31079689	4	60	theme	Rha	710:712	arg1	ratios					720:725	Rha molar ratios	710:725	Rha molar ratios	710:725	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31079689	4	60	theme	Rha	710:712	arg1	LM-POSs					769:775	LM-POSs	769:775	LM-POSs	769:775	The results showed that the antiglycation activity of POSs in vitro was proportional to the galacturonic acid content and GalA:Rha molar ratios except for the low molecular weight POSs (LM-POSs), and inversely proportional to its branching degree, such as Ara:Rha and Gal:Rha molar ratios.
31591153	0	0	theme	Outer	70:74	arg1	Mucilage					76:83	Seed Outer Mucilage	65:83	Seed Outer Mucilage	65:83	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	5	1	theme	Natural	764:770	arg1	variants					772:779	Natural variants	764:779	Natural variants with high molar mass	764:800	Natural variants with high molar mass were associated with a gene encoding a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70).
31591153	4	2	theme	genome	687:692	arg1	polymorphisms					694:706	several genome polymorphisms	679:706	several genome polymorphisms	679:706	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	1	3	theme	Arabidopsis	135:145	arg1	seeds					157:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
31591153	6	4	theme	muci70	906:911	arg1	mutants					923:929	muci70 insertion mutants	906:929	muci70 insertion mutants	906:929	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	0	5	theme	Seed	65:68	arg1	Mucilage					76:83	Seed Outer Mucilage	65:83	Seed Outer Mucilage	65:83	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	7	6	theme	amine	1117:1121	arg1	oxidase					1123:1129	a putative copper amine oxidase	1099:1129	a putative copper amine oxidase of clade 1a (CuAOα1)	1099:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	6	7	from	polymorphism	1021:1032	arg1	gene					1042:1045	this gene	1037:1045	this gene	1037:1045	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	1	8	theme	thaliana	147:154	arg1	seeds					157:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
31591153	6	9	theme	insertion	913:921	arg1	mutants					923:929	muci70 insertion mutants	906:929	muci70 insertion mutants	906:929	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	7	10	theme	copper	1110:1115	arg1	oxidase					1123:1129	a putative copper amine oxidase	1099:1129	a putative copper amine oxidase of clade 1a (CuAOα1)	1099:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	8	11	theme	mutant	1311:1316	arg1	phenotype					1318:1326	the mutant phenotype	1307:1326	the mutant phenotype	1307:1326	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	8	11	theme	mutant	1311:1316	arg1	unique					1331:1336	unique	1331:1336	unique	1331:1336	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	3	12	theme	outer	530:534	arg1	function					545:552	outer mucilage function	530:552	outer mucilage function	530:552	This mucilage is almost exclusively made up of rhamnogalacturonan I (RGI), highlighting the importance of this pectin for outer mucilage function.
31591153	4	13	theme	genome-wide	560:570	arg1	association					572:582	a genome-wide association	558:582	a genome-wide association study	558:588	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	3	14	theme	mucilage	536:543	arg1	function					545:552	outer mucilage function	530:552	outer mucilage function	530:552	This mucilage is almost exclusively made up of rhamnogalacturonan I (RGI), highlighting the importance of this pectin for outer mucilage function.
31591153	4	15	attach	linked	669:674	arg2	variations					600:609	observed variations	591:609	observed variations in polymer amount and macromolecular characteristics	591:662	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	4	15	attach	linked	669:674	arg1	polymorphisms					694:706	several genome polymorphisms	679:706	several genome polymorphisms	679:706	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	4	15	attach	linked	669:674	arg3	study					584:588	a genome-wide association study	558:588	a genome-wide association study	558:588	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	4	16	theme	regulation	752:761	arg1	complexity					724:733	the complexity	720:733	the complexity of their genetic regulation	720:761	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	3	17	theme	pectin	519:524	arg1	importance					500:509	the importance	496:509	the importance of this pectin for outer mucilage function	496:552	This mucilage is almost exclusively made up of rhamnogalacturonan I (RGI), highlighting the importance of this pectin for outer mucilage function.
31591153	8	18	with	unique	1331:1336	arg1	production					1348:1357	RGI production	1344:1357	RGI production only impaired for outer mucilage	1344:1390	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	4	19	theme	several	679:685	arg1	polymorphisms					694:706	several genome polymorphisms	679:706	several genome polymorphisms	679:706	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	7	20	theme	cuaoα1	1248:1253	arg1	mutants					1255:1261	cuaoα1 mutants	1248:1261	cuaoα1 mutants	1248:1261	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	2	21	theme	natural	374:380	arg1	variation					382:390	little natural variation	367:390	little natural variation in composition	367:405	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	0	22	theme	Underlying	89:98	arg1	Genes					100:104	Underlying Genes	89:104	Underlying Genes	89:104	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	0	23	from	Role	32:35	arg1	Mucilage					76:83	Seed Outer Mucilage	65:83	Seed Outer Mucilage	65:83	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	0	23	from	Role	32:35	arg1	Genes					100:104	Underlying Genes	89:104	Underlying Genes	89:104	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	7	24	theme	putative	1101:1108	arg1	oxidase					1123:1129	a putative copper amine oxidase	1099:1129	a putative copper amine oxidase of clade 1a (CuAOα1)	1099:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	25	from	layer	1241:1245	arg1	present					1211:1217	present	1211:1217	present	1211:1217	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	2	26	theme	little	367:372	arg1	variation					382:390	little natural variation	367:390	little natural variation in composition	367:405	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	8	27	theme	outer	1377:1381	arg1	mucilage					1383:1390	outer mucilage	1377:1390	outer mucilage	1377:1390	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	2	28	theme	set	303:305	arg1	Analysis					265:272	Analysis	265:272	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions	265:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	7	29	theme	pectin	1285:1290	arg1	production					1292:1301	pectin production	1285:1301	pectin production	1285:1301	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	0	30	theme	Natural	0:6	arg1	Variation					8:16	Natural Variation	0:16	Natural Variation	0:16	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	7	31	from	present	1211:1217	arg1	layer					1241:1245	the outer mucilage layer	1222:1245	the outer mucilage layer	1222:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	4	32	theme	macromolecular	633:646	arg1	characteristics					648:662	macromolecular characteristics	633:662	macromolecular characteristics	633:662	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	2	33	theme	data	298:301	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	2	33	theme	data	298:301	arg1	set					303:305	a publicly available data set	277:305	a publicly available data set of outer seed mucilage traits of over 300 accessions	277:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	2	34	theme	accessions	349:358	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	2	35	theme	available	288:296	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	2	35	theme	available	288:296	arg1	set					303:305	a publicly available data set	277:305	a publicly available data set of outer seed mucilage traits of over 300 accessions	277:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	6	36	theme	polymer	1062:1068	arg1	size					1070:1073	RGI polymer size	1058:1073	RGI polymer size	1058:1073	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	7	37	theme	second	1078:1083	arg1	gene					1085:1088	A second gene	1076:1088	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1)	1076:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	8	38	theme	further	1437:1443	arg1	mechanism					1445:1453	a further mechanism	1435:1453	a further mechanism controlling mucilage synthesis	1435:1484	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	2	39	theme	traits	330:335	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	2	39	theme	traits	330:335	arg1	set					303:305	a publicly available data set	277:305	a publicly available data set of outer seed mucilage traits of over 300 accessions	277:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	6	40	theme	RGI	1058:1060	arg1	size					1070:1073	RGI polymer size	1058:1073	RGI polymer size	1058:1073	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	7	41	theme	natural	1172:1178	arg1	variation					1180:1188	natural variation	1172:1188	natural variation in the amount of RGI present in the outer mucilage layer	1172:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	42	theme	outer	1226:1230	arg1	layer					1241:1245	the outer mucilage layer	1222:1245	the outer mucilage layer	1222:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	4	43	from	variations	600:609	arg1	amount					622:627	polymer amount	614:627	polymer amount	614:627	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	4	43	from	variations	600:609	arg1	characteristics					648:662	macromolecular characteristics	633:662	macromolecular characteristics	633:662	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	1	44	theme	epidermal	198:206	arg1	cells					208:212	their epidermal cells	192:212	their epidermal cells that form a two-layered hydrogel, termed mucilage	192:262	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
31591153	0	45	theme	Key	28:30	arg1	Role					32:35	a Key Role	26:35	a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes	26:104	Natural Variation Reveals a Key Role for Rhamnogalacturonan I in Seed Outer Mucilage and Underlying Genes.
31591153	5	46	theme	high	786:789	arg1	mass					797:800	high molar mass	786:800	high molar mass	786:800	Natural variants with high molar mass were associated with a gene encoding a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70).
31591153	4	47	theme	polymer	614:620	arg1	amount					622:627	polymer amount	614:627	polymer amount	614:627	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	5	48	theme	molar	791:795	arg1	mass					797:800	high molar mass	786:800	high molar mass	786:800	Natural variants with high molar mass were associated with a gene encoding a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70).
31591153	7	49	theme	present	1211:1217	arg1	RGI					1207:1209	RGI	1207:1209	RGI	1207:1209	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	49	theme	present	1211:1217	arg1	amount					1197:1202	the amount	1193:1202	the amount of RGI present in the outer mucilage layer	1193:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	1	50	from	cells	208:212	arg1	polysaccharides					171:185	polysaccharides	171:185	polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage	171:262	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
31591153	2	51	theme	mucilage	321:328	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	7	52	theme	1a	1140:1141	arg1	oxidase					1123:1129	a putative copper amine oxidase	1099:1129	a putative copper amine oxidase of clade 1a (CuAOα1)	1099:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	6	53	theme	RGI	951:953	arg1	polymers					955:962	many short RGI polymers	940:962	many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size	940:1073	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	8	54	theme	mucilage	1467:1474	arg1	synthesis					1476:1484	mucilage synthesis	1467:1484	mucilage synthesis	1467:1484	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	8	55	theme	impaired	1364:1371	arg1	production					1348:1357	RGI production	1344:1357	RGI production only impaired for outer mucilage	1344:1390	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	7	56	from	role	1277:1280	arg1	production					1292:1301	pectin production	1285:1301	pectin production	1285:1301	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	2	57	theme	seed	316:319	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	6	58	theme	short	945:949	arg1	polymers					955:962	many short RGI polymers	940:962	many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size	940:1073	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	7	59	from	variation	1180:1188	arg1	RGI					1207:1209	RGI	1207:1209	RGI	1207:1209	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	59	from	variation	1180:1188	arg1	amount					1197:1202	the amount	1193:1202	the amount of RGI present in the outer mucilage layer	1193:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	2	60	theme	outer	310:314	arg1	traits					330:335	outer seed mucilage traits	310:335	outer seed mucilage traits of over 300 accessions	310:358	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	4	61	theme	genetic	744:750	arg1	regulation					752:761	their genetic regulation	738:761	their genetic regulation	738:761	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	5	62	with	variants	772:779	arg1	mass					797:800	high molar mass	786:800	high molar mass	786:800	Natural variants with high molar mass were associated with a gene encoding a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70).
31591153	7	63	attach	present	1211:1217	arg2	RGI					1207:1209	RGI	1207:1209	RGI	1207:1209	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	63	attach	present	1211:1217	arg2	amount					1197:1202	the amount	1193:1202	the amount of RGI present in the outer mucilage layer	1193:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	63	attach	present	1211:1217	arg1	layer					1241:1245	the outer mucilage layer	1222:1245	the outer mucilage layer	1222:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	4	64	theme	association	572:582	arg1	study					584:588	a genome-wide association study	558:588	a genome-wide association study	558:588	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	7	65	theme	RGI	1207:1209	arg1	RGI					1207:1209	RGI	1207:1209	RGI	1207:1209	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	65	theme	RGI	1207:1209	arg1	amount					1197:1202	the amount	1193:1202	the amount of RGI present in the outer mucilage layer	1193:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	5	66	theme	putative	841:848	arg1	glycosyltransferase					850:868	a putative glycosyltransferase	839:868	a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70)	839:903	Natural variants with high molar mass were associated with a gene encoding a putative glycosyltransferase called MUCILAGE-RELATED70 (MUCI70).
31591153	2	67	from	variation	382:390	arg1	composition					395:405	composition	395:405	composition	395:405	Analysis of a publicly available data set of outer seed mucilage traits of over 300 accessions showed little natural variation in composition.
31591153	1	68	theme	Arabidopsis	122:132	arg1	seeds					157:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	Arabidopsis (Arabidopsis thaliana) seeds	122:161	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
31591153	8	69	theme	RGI	1344:1346	arg1	production					1348:1357	RGI production	1344:1357	RGI production only impaired for outer mucilage	1344:1390	As the mutant phenotype is unique, with RGI production only impaired for outer mucilage, this indicates that CuAOα1 contributes to a further mechanism controlling mucilage synthesis.
31591153	4	70	theme	observed	591:598	arg1	variations					600:609	observed variations	591:609	observed variations in polymer amount and macromolecular characteristics	591:662	In a genome-wide association study, observed variations in polymer amount and macromolecular characteristics were linked to several genome polymorphisms, indicating the complexity of their genetic regulation.
31591153	7	71	theme	clade	1134:1138	arg1	CuAOα1					1144:1149	CuAOα1	1144:1149	CuAOα1	1144:1149	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	7	71	theme	clade	1134:1138	arg1	1a					1140:1141	clade 1a	1134:1141	clade 1a (CuAOα1)	1134:1150	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	6	72	theme	many	940:943	arg1	polymers					955:962	many short RGI polymers	940:962	many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size	940:1073	muci70 insertion mutants produced many short RGI polymers that were highly substituted with xylan, confirming that polymorphism in this gene can affect RGI polymer size.
31591153	7	73	theme	mucilage	1232:1239	arg1	layer					1241:1245	the outer mucilage layer	1222:1245	the outer mucilage layer	1222:1245	A second gene encoding a putative copper amine oxidase of clade 1a (CuAOα1) was associated with natural variation in the amount of RGI present in the outer mucilage layer; cuaoα1 mutants validated its role in pectin production.
31591153	1	74	theme	two-layered	226:236	arg1	hydrogel					238:245	a two-layered hydrogel	224:245	a two-layered hydrogel	224:245	On imbibition, Arabidopsis (Arabidopsis thaliana) seeds release polysaccharides from their epidermal cells that form a two-layered hydrogel, termed mucilage.
32089591	6	0	theme	Escherichia	1108:1118	arg1	O157					1125:1128	enterohemorrhagic Escherichia coli O157	1090:1128	enterohemorrhagic Escherichia coli O157:H7	1090:1131	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	2	1	theme	direct	360:365	arg1	action					381:386	direct antimicrobial action	360:386	direct antimicrobial action - likely via a decoy effect -	360:416	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	6	2	theme	fucosylated	1050:1060	arg1	BMO					1062:1064	fucosylated BMO	1050:1064	fucosylated BMO	1050:1064	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	6	3	theme	epithelial	1153:1162	arg1	cells					1171:1175	human intestinal epithelial Caco-2 cells	1136:1175	human intestinal epithelial Caco-2 cells	1136:1175	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	3	4	dep	HMO	578:580	arg1	resemble					569:576	resemble	569:576	resemble	569:576	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	1	5	theme	breastfed	257:265	arg1	infants					267:273	breastfed infants	257:273	breastfed infants	257:273	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	0	6	theme	milk	100:103	arg1	oligosaccharides					105:120	bovine milk oligosaccharides	93:120	bovine milk oligosaccharides	93:120	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	4	7	theme	HMO-like	769:776	arg1	glycans					778:784	HMO-like glycans	769:784	HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches	769:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	2	8	theme	human	303:307	arg1	oligosaccharides					314:329	sialylated human milk oligosaccharides	292:329	sialylated human milk oligosaccharides	292:329	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	3	9	theme	BMO	565:567	arg1	HMO					578:580	BMO resemble HMO	565:580	BMO resemble HMO	565:580	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	0	10	theme	bovine	93:98	arg1	oligosaccharides					105:120	bovine milk oligosaccharides	93:120	bovine milk oligosaccharides	93:120	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	3	11	theme	HMO-mimics	551:560	arg1	oligosaccharides					502:517	Bovine milk oligosaccharides	490:517	Bovine milk oligosaccharides (BMO)	490:523	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	11	theme	HMO-mimics	551:560	arg1	source					541:546	a potential source	529:546	a potential source of HMO-mimics	529:560	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	4	12	dep	whey	684:687	arg1	permeate					689:696	permeate	689:696	permeate	689:696	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	2	13	theme	sialylated	292:301	arg1	oligosaccharides					314:329	sialylated human milk oligosaccharides	292:329	sialylated human milk oligosaccharides	292:329	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	3	14	gly	fucosylated	619:629	arg1	structures					631:640	simpler and less fucosylated structures	602:640	simpler and less fucosylated structures	602:640	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	6	15	theme	intestinal	1142:1151	arg1	cells					1171:1175	human intestinal epithelial Caco-2 cells	1136:1175	human intestinal epithelial Caco-2 cells	1136:1175	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	0	16	gly	glycosylation	76:88	arg1	oligosaccharides					105:120	bovine milk oligosaccharides	93:120	bovine milk oligosaccharides	93:120	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	1	17	theme	reduced	171:177	arg1	incidence					179:187	incidence	179:187	incidence	179:187	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	0	18	theme	oligosaccharides	105:120	arg1	glycosylation					76:88	enzymatic glycosylation	66:88	enzymatic glycosylation of bovine milk oligosaccharides	66:120	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	6	19	theme	Caco-2	1164:1169	arg1	cells					1171:1175	human intestinal epithelial Caco-2 cells	1136:1175	human intestinal epithelial Caco-2 cells	1136:1175	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	6	20	theme	human	1136:1140	arg1	cells					1171:1175	human intestinal epithelial Caco-2 cells	1136:1175	human intestinal epithelial Caco-2 cells	1136:1175	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	2	21	theme	likely	390:395	arg1	action					381:386	direct antimicrobial action	360:386	direct antimicrobial action - likely via a decoy effect -	360:416	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	6	22	theme	activity	1019:1026	arg1	assays					1028:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	6	23	dep	O157	1125:1128	arg1	H7					1130:1131	H7	1130:1131	enterohemorrhagic Escherichia coli O157:H7	1090:1131	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	4	24	theme	large	659:663	arg1	scales					665:670	large scales	659:670	large scales	659:670	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	0	25	theme	mimics	25:30	arg1	Production					0:9	Production	0:9	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.	0:121	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	6	26	theme	anti-adherence	1004:1017	arg1	assays					1028:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	5	27	theme	identical	956:964	arg1	composition					966:976	identical composition	956:976	identical composition	956:976	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	5	28	theme	known	981:985	arg1	HMO					987:989	known HMO	981:989	known HMO	981:989	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	0	29	theme	functional	14:23	arg1	mimics					25:30	functional mimics	14:30	functional mimics of human milk oligosaccharides	14:61	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	6	30	theme	Preliminary	992:1002	arg1	assays					1028:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays	992:1033	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	3	31	theme	potential	531:539	arg1	oligosaccharides					502:517	Bovine milk oligosaccharides	490:517	Bovine milk oligosaccharides (BMO)	490:523	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	31	theme	potential	531:539	arg1	source					541:546	a potential source	529:546	a potential source of HMO-mimics	529:560	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	4	32	attach	isolated	647:654	arg1	whey					684:687	whey	684:687	whey	684:687	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	4	32	attach	isolated	647:654	arg2	BMO					643:645	BMO	643:645	BMO isolated at large scales from bovine whey permeate	643:696	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	2	33	theme	milk	309:312	arg1	oligosaccharides					314:329	sialylated human milk oligosaccharides	292:329	sialylated human milk oligosaccharides	292:329	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	1	34	theme	enteric	205:211	arg1	infections					213:222	enteric infections	205:222	enteric infections	205:222	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	0	35	theme	human	35:39	arg1	oligosaccharides					46:61	human milk oligosaccharides	35:61	human milk oligosaccharides	35:61	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	5	36	theme	Quadrupole	854:863	arg1	analysis					886:893	Quadrupole time-of-flight LC/MS analysis	854:893	Quadrupole time-of-flight LC/MS analysis	854:893	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	4	37	theme	cost-effective	807:820	arg1	approaches					842:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	6	38	theme	O157	1125:1128	arg1	uptake					1080:1085	the uptake	1076:1085	the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells	1076:1175	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	1	39	theme	infections	213:222	arg1	severity					193:200	severity	193:200	severity	193:200	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	1	39	theme	infections	213:222	arg1	incidence					179:187	incidence	179:187	incidence	179:187	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	5	40	theme	LC/MS	880:884	arg1	analysis					886:893	Quadrupole time-of-flight LC/MS analysis	854:893	Quadrupole time-of-flight LC/MS analysis	854:893	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	3	41	theme	fucosylated	619:629	arg1	structures					631:640	simpler and less fucosylated structures	602:640	simpler and less fucosylated structures	602:640	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	2	42	theme	antimicrobial	431:443	arg1	action					445:450	indirect antimicrobial action	422:450	indirect antimicrobial action	422:450	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	2	43	theme	decoy	403:407	arg1	effect					409:414	a decoy effect	401:414	a decoy effect	401:414	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	0	44	theme	oligosaccharides	46:61	arg1	mimics					25:30	functional mimics	14:30	functional mimics of human milk oligosaccharides	14:61	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	2	45	gly	sialylated	292:301	arg1	oligosaccharides					314:329	sialylated human milk oligosaccharides	292:329	sialylated human milk oligosaccharides	292:329	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	4	46	theme	sialic	745:750	arg1	acid					752:755	sialic acid	745:755	sialic acid	745:755	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	4	47	theme	multienzyme	830:840	arg1	approaches					842:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	5	48	contain	had	952:954	arg1	9					950:950	9	950:950	9	950:950	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	5	48	contain	had	952:954	arg2	composition					966:976	identical composition	956:976	identical composition	956:976	Quadrupole time-of-flight LC/MS analysis revealed that 22 oligosaccharides were synthesized and 9 had identical composition to known HMO.
32089591	0	49	theme	milk	41:44	arg1	oligosaccharides					46:61	human milk oligosaccharides	35:61	human milk oligosaccharides	35:61	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	1	50	from	morbidity	244:252	arg1	infants					267:273	breastfed infants	257:273	breastfed infants	257:273	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	2	51	theme	commensal	460:468	arg1	enhancement					477:487	commensal growth enhancement	460:487	commensal growth enhancement	460:487	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	4	52	theme	one-pot	822:828	arg1	approaches					842:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	3	53	theme	simpler	602:608	arg1	structures					631:640	simpler and less fucosylated structures	602:640	simpler and less fucosylated structures	602:640	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	54	theme	Bovine	490:495	arg1	oligosaccharides					502:517	Bovine milk oligosaccharides	490:517	Bovine milk oligosaccharides (BMO)	490:523	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	54	theme	Bovine	490:495	arg1	source					541:546	a potential source	529:546	a potential source of HMO-mimics	529:560	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	54	theme	Bovine	490:495	arg1	BMO					520:522	BMO	520:522	BMO	520:522	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	6	55	theme	native	1199:1204	arg1	BMO					1206:1208	native BMO	1199:1208	native BMO	1199:1208	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	6	56	dep	Escherichia	1108:1118	arg1	coli					1120:1123	coli	1120:1123	coli	1120:1123	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	4	57	theme	acid	752:755	arg1	addition					719:726	the addition	715:726	the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches	715:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	4	58	theme	fucose	731:736	arg1	addition					719:726	the addition	715:726	the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches	715:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	3	59	theme	milk	497:500	arg1	oligosaccharides					502:517	Bovine milk oligosaccharides	490:517	Bovine milk oligosaccharides (BMO)	490:523	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	59	theme	milk	497:500	arg1	source					541:546	a potential source	529:546	a potential source of HMO-mimics	529:560	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	59	theme	milk	497:500	arg1	BMO					520:522	BMO	520:522	BMO	520:522	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	60	contain	have	597:600	arg1	they					592:595	they	592:595	they	592:595	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	3	60	contain	have	597:600	arg2	structures					631:640	simpler and less fucosylated structures	602:640	simpler and less fucosylated structures	602:640	Bovine milk oligosaccharides (BMO) are a potential source of HMO-mimics as BMO resemble HMO; however, they have simpler and less fucosylated structures.
32089591	0	61	theme	enzymatic	66:74	arg1	glycosylation					76:88	enzymatic glycosylation	66:88	enzymatic glycosylation of bovine milk oligosaccharides	66:120	Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides.
32089591	4	62	mod	modified	703:710	arg3	addition					719:726	the addition	715:726	the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches	715:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	4	62	mod	modified	703:710	arg1	BMO					643:645	BMO	643:645	BMO isolated at large scales from bovine whey permeate	643:696	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	4	63	theme	high-yield	792:801	arg1	approaches					842:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	high-yield and cost-effective one-pot multienzyme approaches	792:851	BMO isolated at large scales from bovine whey permeate were modified by the addition of fucose and/or sialic acid to generate HMO-like glycans using high-yield and cost-effective one-pot multienzyme approaches.
32089591	1	64	theme	milk	147:150	arg1	Consumption					123:133	Consumption	123:133	Consumption of mothers' milk	123:150	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	1	65	theme	reduced	236:242	arg1	morbidity					244:252	reduced morbidity	236:252	reduced morbidity in breastfed infants	236:273	Consumption of mothers' milk is associated with reduced incidence and severity of enteric infections, leading to reduced morbidity in breastfed infants.
32089591	2	66	theme	growth	470:475	arg1	enhancement					477:487	commensal growth enhancement	460:487	commensal growth enhancement	460:487	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	6	67	gly	fucosylated	1050:1060	arg1	BMO					1062:1064	fucosylated BMO	1050:1064	fucosylated BMO	1050:1064	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
32089591	2	68	theme	indirect	422:429	arg1	action					445:450	indirect antimicrobial action	422:450	indirect antimicrobial action	422:450	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	2	69	theme	antimicrobial	367:379	arg1	action					381:386	direct antimicrobial action	360:386	direct antimicrobial action - likely via a decoy effect -	360:416	Fucosylated and sialylated human milk oligosaccharides (HMO) are important for both direct antimicrobial action - likely via a decoy effect - and indirect antimicrobial action through commensal growth enhancement.
32089591	6	70	theme	enterohemorrhagic	1090:1106	arg1	O157					1125:1128	enterohemorrhagic Escherichia coli O157	1090:1128	enterohemorrhagic Escherichia coli O157:H7	1090:1131	Preliminary anti-adherence activity assays indicated that fucosylated BMO decreased the uptake of enterohemorrhagic Escherichia coli O157:H7 by human intestinal epithelial Caco-2 cells more effectively than native BMO.
29415416	0	0	theme	cellulose	98:106	arg1	nanowhiskers					108:119	cellulose nanowhiskers	98:119	cellulose nanowhiskers for bone tissue engineering applications	98:160	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	5	1	theme	FTIR	806:809	arg1	analysis					811:818	FTIR analysis	806:818	FTIR analysis	806:818	FTIR analysis confirmed the interaction between CCNWs and AgNPs.
29415416	6	2	theme	80-90	965:969	arg1	%					970:970	%	970:970	%	970:970	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	7	3	theme	swelling	1149:1156	arg1	capacity					1158:1165	The swelling capacity	1145:1165	The swelling capacity of scaffolds	1145:1178	The swelling capacity of scaffolds decreased after the incorporation of nanocomposite.
29415416	4	4	theme	XRD	663:665	arg1	analysis					677:684	XRD and FESEM analysis	663:684	XRD and FESEM analysis of nanocomposite	663:701	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	7	5	theme	nanocomposite	1217:1229	arg1	incorporation					1200:1212	the incorporation	1196:1212	the incorporation of nanocomposite	1196:1229	The swelling capacity of scaffolds decreased after the incorporation of nanocomposite.
29415416	11	6	theme	antimicrobial	1646:1658	arg1	scaffolds					1660:1668	self-sustained and potential antimicrobial scaffolds	1617:1668	scaffolds	1660:1668	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	8	7	theme	scaffold	1241:1248	arg1	rate					1262:1265	scaffold degradation rate	1241:1265	scaffold degradation rate	1241:1265	In turn, scaffold degradation rate was tuned to support angiogenesis and vascularization.
29415416	6	8	theme	%	970:970	arg1	porosity					972:979	the desirable 80-90% porosity	951:979	the desirable 80-90% porosity	951:979	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	4	9	theme	long	786:789	arg1	surface					797:803	~200 nm long CCNWs surface	778:803	~200 nm long CCNWs surface	778:803	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	1	10	theme	mechanical	291:300	arg1	strength					302:309	mechanical strength	291:309	mechanical strength	291:309	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	3	11	contain	containing	522:531	arg2	cellulose					565:573	carboxymethyl cellulose	551:573	carboxymethyl cellulose (CMC)	551:579	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	3	11	contain	containing	522:531	arg1	Scaffolds					512:520	Scaffolds	512:520	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite	512:617	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	3	11	contain	containing	522:531	arg2	CMC					576:578	CMC	576:578	CMC	576:578	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	3	11	contain	containing	522:531	arg2	CS					543:544	CS	543:544	CS	543:544	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	3	11	contain	containing	522:531	arg2	chitosan					533:540	chitosan	533:540	chitosan (CS)	533:545	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	10	12	theme	bone	1513:1516	arg1	growth					1518:1523	bone growth	1513:1523	bone growth	1513:1523	Incorporation of CCNWs also resulted in improved biomineralization for bone growth.
29415416	0	13	theme	tissue	130:135	arg1	applications					149:160	bone tissue engineering applications	125:160	bone tissue engineering applications	125:160	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	6	14	theme	desirable	955:963	arg1	porosity					972:979	the desirable 80-90% porosity	951:979	the desirable 80-90% porosity	951:979	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	7	15	theme	scaffolds	1170:1178	arg1	capacity					1158:1165	The swelling capacity	1145:1165	The swelling capacity of scaffolds	1145:1178	The swelling capacity of scaffolds decreased after the incorporation of nanocomposite.
29415416	2	16	theme	dual	436:439	arg1	functions					441:449	dual functions	436:449	dual functions of providing mechanical strength and antimicrobial activity	436:509	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	4	17	theme	nanocomposite	689:701	arg1	analysis					677:684	XRD and FESEM analysis	663:684	XRD and FESEM analysis of nanocomposite	663:701	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	0	18	theme	bone	125:128	arg1	applications					149:160	bone tissue engineering applications	125:160	bone tissue engineering applications	125:160	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	11	19	theme	bone	1708:1711	arg1	infections					1721:1730	bone related infections	1708:1730	bone related infections like osteomyelitis	1708:1749	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	0	20	theme	Nano-biocomposite	0:16	arg1	scaffolds					18:26	Nano-biocomposite scaffolds	0:26	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle	0:87	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	3	21	theme	carboxymethyl	551:563	arg1	CMC					576:578	CMC	576:578	CMC	576:578	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	3	21	theme	carboxymethyl	551:563	arg1	cellulose					565:573	carboxymethyl cellulose	551:573	carboxymethyl cellulose (CMC)	551:579	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	11	22	theme	related	1713:1719	arg1	infections					1721:1730	bone related infections	1708:1730	bone related infections like osteomyelitis	1708:1749	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	11	23	theme	excellent	1559:1567	arg1	properties					1569:1578	excellent properties	1559:1578	excellent properties of fabricated scaffolds	1559:1602	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	2	24	theme	mechanical	464:473	arg1	strength					475:482	mechanical strength	464:482	mechanical strength	464:482	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	6	25	theme	mechanical	1035:1044	arg1	strength					1046:1053	mechanical strength	1035:1053	mechanical strength	1035:1053	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	6	26	theme	bone	1139:1142	arg1	strength					1116:1123	the mechanical strength	1101:1123	the mechanical strength of cancellous bone	1101:1142	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	0	27	theme	chitosan	31:38	arg1	scaffolds					18:26	Nano-biocomposite scaffolds	0:26	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle	0:87	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	8	28	theme	degradation	1250:1260	arg1	rate					1262:1265	scaffold degradation rate	1241:1265	scaffold degradation rate	1241:1265	In turn, scaffold degradation rate was tuned to support angiogenesis and vascularization.
29415416	11	29	theme	loaded	1683:1688	arg1	drug					1690:1693	any loaded drug	1679:1693	any loaded drug	1679:1693	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	4	30	theme	CCNWs	791:795	arg1	surface					797:803	~200 nm long CCNWs surface	778:803	~200 nm long CCNWs surface	778:803	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	6	31	theme	cancellous	1128:1137	arg1	bone					1139:1142	cancellous bone	1128:1142	cancellous bone	1128:1142	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	0	32	theme	engineering	137:147	arg1	applications					149:160	bone tissue engineering applications	125:160	bone tissue engineering applications	125:160	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	3	33	theme	varying	586:592	arg1	percent					594:600	varying percent	586:600	varying percent of nanocomposite	586:617	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	11	34	theme	fabricated	1583:1592	arg1	scaffolds					1594:1602	fabricated scaffolds	1583:1602	fabricated scaffolds	1583:1602	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	1	35	theme	efficient	214:222	arg1	scaffolds					249:257	highly efficient nano-composite polymeric scaffolds	207:257	highly efficient nano-composite polymeric scaffolds	207:257	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	0	36	theme	carboxymethyl	41:53	arg1	cellulose					55:63	carboxymethyl cellulose	41:63	carboxymethyl cellulose	41:63	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	11	37	theme	scaffolds	1594:1602	arg1	properties					1569:1578	excellent properties	1559:1578	excellent properties of fabricated scaffolds	1559:1602	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	1	38	theme	nano-composite	224:237	arg1	scaffolds					249:257	highly efficient nano-composite polymeric scaffolds	207:257	highly efficient nano-composite polymeric scaffolds	207:257	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	1	39	theme	polymeric	239:247	arg1	scaffolds					249:257	highly efficient nano-composite polymeric scaffolds	207:257	highly efficient nano-composite polymeric scaffolds	207:257	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	2	40	theme	antimicrobial	488:500	arg1	activity					502:509	antimicrobial activity	488:509	antimicrobial activity	488:509	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	6	41	theme	mechanical	1105:1114	arg1	strength					1116:1123	the mechanical strength	1101:1123	the mechanical strength of cancellous bone	1101:1142	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	6	42	theme	scaffolds	909:917	arg1	preparation					919:929	scaffolds preparation	909:929	scaffolds preparation	909:929	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	3	43	theme	nanocomposite	605:617	arg1	percent					594:600	varying percent	586:600	varying percent of nanocomposite	586:617	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	10	44	theme	CCNWs	1459:1463	arg1	Incorporation					1442:1454	Incorporation	1442:1454	Incorporation of CCNWs	1442:1463	Incorporation of CCNWs also resulted in improved biomineralization for bone growth.
29415416	0	45	theme	cellulose	55:63	arg1	scaffolds					18:26	Nano-biocomposite scaffolds	0:26	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle	0:87	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	9	46	theme	excellent	1354:1362	arg1	activity					1378:1385	excellent antimicrobial activity	1354:1385	excellent antimicrobial activity	1354:1385	Scaffolds apart from exhibiting excellent antimicrobial activity, also supported MG63 cells adhesion and proliferation.
29415416	9	47	theme	antimicrobial	1364:1376	arg1	activity					1378:1385	excellent antimicrobial activity	1354:1385	excellent antimicrobial activity	1354:1385	Scaffolds apart from exhibiting excellent antimicrobial activity, also supported MG63 cells adhesion and proliferation.
29415416	6	48	theme	nanocomposite	888:900	arg1	Incorporation					871:883	Incorporation	871:883	Incorporation of nanocomposite during scaffolds preparation	871:929	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	4	49	theme	crystalline	719:729	arg1	structure					731:739	highly crystalline structure	712:739	highly crystalline structure	712:739	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	2	50	theme	nanoparticles	362:374	arg1	CCNWs-AgNPs					339:349	CCNWs-AgNPs	339:349	CCNWs-AgNPs	339:349	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	2	50	theme	nanoparticles	362:374	arg1	nanocomposite					324:336	nanocomposite	324:336	nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity	324:509	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	2	50	theme	nanoparticles	362:374	arg1	nanoparticles					362:374	silver nanoparticles	355:374	silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs)	355:421	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	11	51	theme	potential	1636:1644	arg1	scaffolds					1660:1668	self-sustained and potential antimicrobial scaffolds	1617:1668	scaffolds	1660:1668	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	9	52	theme	cells	1408:1412	arg1	adhesion					1414:1421	MG63 cells adhesion	1403:1421	MG63 cells adhesion	1403:1421	Scaffolds apart from exhibiting excellent antimicrobial activity, also supported MG63 cells adhesion and proliferation.
29415416	2	53	theme	silver	355:360	arg1	AgNPs					377:381	AgNPs	377:381	AgNPs	377:381	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	2	53	theme	silver	355:360	arg1	nanoparticles					362:374	silver nanoparticles	355:374	silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs)	355:421	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	0	54	theme	nanoparticle	76:87	arg1	scaffolds					18:26	Nano-biocomposite scaffolds	0:26	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle	0:87	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	4	55	theme	FESEM	671:675	arg1	analysis					677:684	XRD and FESEM analysis	663:684	XRD and FESEM analysis of nanocomposite	663:701	XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface.
29415416	3	56	theme	freeze	641:646	arg1	method					655:660	freeze drying method	641:660	freeze drying method	641:660	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	10	57	theme	improved	1482:1489	arg1	biomineralization					1491:1507	improved biomineralization	1482:1507	improved biomineralization for bone growth	1482:1523	Incorporation of CCNWs also resulted in improved biomineralization for bone growth.
29415416	2	58	theme	carboxylated	397:408	arg1	CCNWs					416:420	CCNWs	416:420	CCNWs	416:420	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	2	58	theme	carboxylated	397:408	arg1	CNWs					410:413	carboxylated CNWs	397:413	carboxylated CNWs (CCNWs)	397:421	We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity.
29415416	11	59	theme	self-sustained	1617:1630	arg1	scaffolds					1660:1668	self-sustained and potential antimicrobial scaffolds	1617:1668	scaffolds	1660:1668	Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis.
29415416	1	60	theme	controllable	264:275	arg1	size					282:285	controllable pore size	264:285	controllable pore size	264:285	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	0	61	theme	silver	69:74	arg1	nanoparticle					76:87	silver nanoparticle	69:87	silver nanoparticle	69:87	Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
29415416	6	62	theme	pore	986:989	arg1	diameter					991:998	pore diameter	986:998	pore diameter ranging between 150 and 500 μm	986:1029	Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone.
29415416	3	63	theme	drying	648:653	arg1	method					655:660	freeze drying method	641:660	freeze drying method	641:660	Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method.
29415416	1	64	theme	present	170:176	arg1	work					178:181	the present work	166:181	the present work	166:181	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
29415416	1	65	theme	pore	277:280	arg1	size					282:285	controllable pore size	264:285	controllable pore size	264:285	In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength.
31247231	0	0	theme	Water	89:93	arg1	chestnut					95:102	Water chestnut	89:102	Water chestnut (Trapa bispinosa)	89:120	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	0	0	theme	Water	89:93	arg1	bispinosa					111:119	Trapa bispinosa	105:119	Trapa bispinosa	105:119	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	4	1	theme	chestnut	465:472	arg1	starch					474:479	water chestnut starch	459:479	water chestnut starch	459:479	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	5	2	theme	While	609:613	arg1	starch					639:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	7	3	theme	burst	945:949	arg1	strength					951:958	burst strength	945:958	burst strength of native starch	945:975	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	1	4	theme	physicochemical	225:239	arg1	properties					241:250	various physicochemical properties	217:250	various physicochemical properties	217:250	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	8	5	theme	moisture	1100:1107	arg1	content					1109:1115	thickness (0.041 ± 0.07 mm) moisture content	1072:1115	thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%)	1072:1130	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	5	theme	moisture	1100:1107	arg1	%					1129:1129	4.17 ± 0.32%	1118:1129	4.17 ± 0.32%	1118:1129	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	9	6	theme	transmission	1398:1409	arg1	rate					1411:1414	water vapour transmission rate	1385:1414	water vapour transmission rate	1385:1414	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	6	7	theme	nanoparticles	794:806	arg1	concentrations					769:782	varying concentrations	761:782	varying concentrations of starch nanoparticles (SNPs)	761:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	7	8	theme	transmission	908:919	arg1	rate					921:924	water vapour transmission rate	895:924	water vapour transmission rate	895:924	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	6	9	theme	solution	837:844	arg1	method					854:859	a solution casting method	835:859	a solution casting method	835:859	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	5	10	theme	irregular	684:692	arg1	surface					704:710	irregular and rough surface	684:710	irregular and rough surface	684:710	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	2	11	contain	having	335:340	arg1	method					328:333	the acid hydrolysis method	308:333	the acid hydrolysis method having a yield of 27.5%	308:357	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	2	11	contain	having	335:340	arg2	yield					344:348	a yield	342:348	a yield of 27.5%	342:357	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	5	12	theme	water	619:623	arg1	starch					639:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	6	13	theme	starch	787:792	arg1	SNPs					809:812	SNPs	809:812	SNPs	809:812	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	6	13	theme	starch	787:792	arg1	nanoparticles					794:806	starch nanoparticles	787:806	starch nanoparticles (SNPs)	787:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	9	14	theme	SNPs	1497:1500	arg1	concentration					1480:1492	the concentration	1476:1492	the concentration of SNPs which is essential features of a good package	1476:1546	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	9	15	theme	moisture	1367:1374	arg1	content					1376:1382	moisture content	1367:1382	moisture content	1367:1382	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	5	16	theme	chestnut	625:632	arg1	starch					639:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	9	17	theme	package	1540:1546	arg1	features					1521:1528	essential features	1511:1528	essential features of a good package	1511:1546	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	8	18	contain	had	1068:1070	arg2	%					1225:1225	35.71 ± 0.17%	1213:1225	35.71 ± 0.17%	1213:1225	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	4.678 × 10-3 ± 0.42 g-2 s-1					1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	976.4 ± 12.47 g					1248:1262	976.4 ± 12.47 g	1248:1262	976.4 ± 12.47 g	1248:1262	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	content					1109:1115	thickness (0.041 ± 0.07 mm) moisture content	1072:1115	thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%)	1072:1130	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg1	film					1063:1066	native starch film	1049:1066	native starch film	1049:1066	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	%					1129:1129	4.17 ± 0.32%	1118:1129	4.17 ± 0.32%	1118:1129	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	rate					1159:1162	water vapour transmission rate	1133:1162	water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1)	1133:1192	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	strength					1238:1245	burst strength	1232:1245	burst strength (976.4 ± 12.47 g)	1232:1263	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	18	contain	had	1068:1070	arg2	solubility					1201:1210	water solubility	1195:1210	water solubility (35.71 ± 0.17%)	1195:1226	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	5	19	theme	rough	698:702	arg1	surface					704:710	irregular and rough surface	684:710	irregular and rough surface	684:710	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	4	20	theme	unique	441:446	arg1	shape					489:493	the shape	485:493	the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface	485:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	4	20	theme	unique	441:446	arg1	feature					448:454	The unique feature	437:454	The unique feature of water chestnut starch	437:479	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	9	21	from	increase	1464:1471	arg1	concentration					1480:1492	the concentration	1476:1492	the concentration of SNPs which is essential features of a good package	1476:1546	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	2	22	theme	Nano	271:274	arg1	starch					276:281	Nano starch	271:281	Nano starch	271:281	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	9	23	theme	vapour	1391:1396	arg1	rate					1411:1414	water vapour transmission rate	1385:1414	water vapour transmission rate	1385:1414	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	8	24	theme	water	1195:1199	arg1	solubility					1201:1210	water solubility	1195:1210	water solubility (35.71 ± 0.17%)	1195:1226	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	24	theme	water	1195:1199	arg1	%					1225:1225	35.71 ± 0.17%	1213:1225	35.71 ± 0.17%	1213:1225	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	0	25	theme	Trapa	105:109	arg1	chestnut					95:102	Water chestnut	89:102	Water chestnut (Trapa bispinosa)	89:120	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	0	25	theme	Trapa	105:109	arg1	bispinosa					111:119	Trapa bispinosa	105:119	Trapa bispinosa	105:119	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	4	26	theme	water	459:463	arg1	starch					474:479	water chestnut starch	459:479	water chestnut starch	459:479	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	3	27	theme	native	390:395	arg1	starch					406:411	native and nano starch	390:411	starch	406:411	Particle size distribution of native and nano starch was 5559 nm and 396 nm.
31247231	6	28	theme	varying	761:767	arg1	concentrations					769:782	varying concentrations	761:782	varying concentrations of starch nanoparticles (SNPs)	761:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	9	29	theme	water	1385:1389	arg1	rate					1411:1414	water vapour transmission rate	1385:1414	water vapour transmission rate	1385:1414	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	3	30	theme	Particle	360:367	arg1	distribution					374:385	Particle size distribution	360:385	Particle size distribution of native and nano starch	360:411	Particle size distribution of native and nano starch was 5559 nm and 396 nm.
31247231	6	31	theme	casting	846:852	arg1	method					854:859	a solution casting method	835:859	a solution casting method	835:859	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	7	32	theme	water	895:899	arg1	rate					921:924	water vapour transmission rate	895:924	water vapour transmission rate	895:924	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	4	33	dep	oval	525:528	arg1	ellipsoidal					531:541	ellipsoidal	531:541	ellipsoidal	531:541	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	3	34	theme	nano	401:404	arg1	starch					406:411	native and nano starch	390:411	starch	406:411	Particle size distribution of native and nano starch was 5559 nm and 396 nm.
31247231	3	35	theme	size	369:372	arg1	distribution					374:385	Particle size distribution	360:385	Particle size distribution of native and nano starch	360:411	Particle size distribution of native and nano starch was 5559 nm and 396 nm.
31247231	7	36	theme	native	963:968	arg1	starch					970:975	native starch	963:975	native starch	963:975	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	9	37	theme	essential	1511:1519	arg1	features					1521:1528	essential features	1511:1528	essential features of a good package	1511:1546	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	3	38	theme	starch	406:411	arg1	distribution					374:385	Particle size distribution	360:385	Particle size distribution of native and nano starch	360:411	Particle size distribution of native and nano starch was 5559 nm and 396 nm.
31247231	8	39	theme	transmission	1146:1157	arg1	4.678 × 10-3 ± 0.42 g-2 s-1					1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	39	theme	transmission	1146:1157	arg1	rate					1159:1162	water vapour transmission rate	1133:1162	water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1)	1133:1192	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	6	40	theme	nanocomposites	735:748	arg1	films					750:754	Water chestnut starch nanocomposites films	713:754	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs)	713:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	7	41	theme	water	927:931	arg1	solubility					933:942	water solubility	927:942	water solubility	927:942	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	1	42	theme	non-conventional	172:187	arg1	source					189:194	a non-conventional source	170:194	a non-conventional source (water chestnut)	170:211	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	1	42	theme	non-conventional	172:187	arg1	chestnut					203:210	water chestnut	197:210	water chestnut	197:210	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	0	43	theme	starch	15:20	arg1	Development					0:10	Development	0:10	Development of starch	0:20	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	5	44	theme	agglomerated	661:672	arg1	form					674:677	an agglomerated form	658:677	an agglomerated form with irregular and rough surface	658:710	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	1	45	attach	isolated	156:163	arg2	starch					145:150	starch	145:150	starch	145:150	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	1	45	attach	isolated	156:163	arg1	source					189:194	a non-conventional source	170:194	a non-conventional source (water chestnut)	170:211	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	1	45	attach	isolated	156:163	arg1	chestnut					203:210	water chestnut	197:210	water chestnut	197:210	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	0	46	theme	composite	42:50	arg1	films					52:56	composite films	42:56	composite films	42:56	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	5	47	with	form	674:677	arg1	surface					704:710	irregular and rough surface	684:710	irregular and rough surface	684:710	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	8	48	theme	starch	1056:1061	arg1	film					1063:1066	native starch film	1049:1066	native starch film	1049:1066	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	9	49	theme	burst	1346:1350	arg1	strength					1352:1359	burst strength	1346:1359	burst strength	1346:1359	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	2	50	theme	%	357:357	arg1	yield					344:348	a yield	342:348	a yield of 27.5%	342:357	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	8	51	theme	native	1049:1054	arg1	film					1063:1066	native starch film	1049:1066	native starch film	1049:1066	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	0	52	dep	films	52:56	arg1	source					80:85	non-conventional source	63:85	non-conventional source	63:85	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	0	52	dep	films	52:56	arg1	chestnut					95:102	Water chestnut	89:102	Water chestnut (Trapa bispinosa)	89:120	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	0	52	dep	films	52:56	arg1	bispinosa					111:119	Trapa bispinosa	105:119	Trapa bispinosa	105:119	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	6	53	theme	starch	728:733	arg1	films					750:754	Water chestnut starch nanocomposites films	713:754	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs)	713:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	1	54	theme	water	197:201	arg1	source					189:194	a non-conventional source	170:194	a non-conventional source (water chestnut)	170:211	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	1	54	theme	water	197:201	arg1	chestnut					203:210	water chestnut	197:210	water chestnut	197:210	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	5	55	theme	nano	634:637	arg1	starch					639:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch	609:644	While the water chestnut nano starch appeared as an agglomerated form with irregular and rough surface.
31247231	6	56	with	films	750:754	arg1	concentrations					769:782	varying concentrations	761:782	varying concentrations of starch nanoparticles (SNPs)	761:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	4	57	dep	looked	518:523	arg1	mixed					544:548	mixed	544:548	mixed with spherical granules without cracks and smooth surface	544:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	9	58	theme	films	1434:1438	arg1	solubility					1420:1429	solubility	1420:1429	solubility of films	1420:1438	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	9	58	theme	films	1434:1438	arg1	rate					1411:1414	water vapour transmission rate	1385:1414	water vapour transmission rate	1385:1414	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	9	58	theme	films	1434:1438	arg1	content					1376:1382	moisture content	1367:1382	moisture content	1367:1382	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	4	59	theme	granule	505:511	arg1	shape					489:493	the shape	485:493	the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface	485:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	4	59	theme	granule	505:511	arg1	feature					448:454	The unique feature	437:454	The unique feature of water chestnut starch	437:479	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	6	60	theme	Water	713:717	arg1	films					750:754	Water chestnut starch nanocomposites films	713:754	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs)	713:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	8	61	theme	burst	1232:1236	arg1	976.4 ± 12.47 g					1248:1262	976.4 ± 12.47 g	1248:1262	976.4 ± 12.47 g	1248:1262	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	61	theme	burst	1232:1236	arg1	strength					1238:1245	burst strength	1232:1245	burst strength (976.4 ± 12.47 g)	1232:1263	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	7	62	theme	moisture	877:884	arg1	content					886:892	moisture content	877:892	moisture content	877:892	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	4	63	theme	starch	498:503	arg1	granule					505:511	starch granule	498:511	starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface	498:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	7	64	theme	starch	986:991	arg1	films					1003:1007	nano starch composite films	981:1007	nano starch composite films	981:1007	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	65	theme	vapour	901:906	arg1	rate					921:924	water vapour transmission rate	895:924	water vapour transmission rate	895:924	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	0	66	dep	nanoparticles	22:34	arg1	based					36:40	based	36:40	nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa)	22:120	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	7	67	theme	starch	970:975	arg1	strength					951:958	burst strength	945:958	burst strength of native starch	945:975	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	67	theme	starch	970:975	arg1	rate					921:924	water vapour transmission rate	895:924	water vapour transmission rate	895:924	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	67	theme	starch	970:975	arg1	content					886:892	moisture content	877:892	moisture content	877:892	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	67	theme	starch	970:975	arg1	films					1003:1007	nano starch composite films	981:1007	nano starch composite films	981:1007	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	67	theme	starch	970:975	arg1	solubility					933:942	water solubility	927:942	water solubility	927:942	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	67	theme	starch	970:975	arg1	thickness					866:874	The thickness	862:874	The thickness	862:874	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	2	68	theme	hydrolysis	317:326	arg1	method					328:333	the acid hydrolysis method	308:333	the acid hydrolysis method having a yield of 27.5%	308:357	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	6	69	theme	chestnut	719:726	arg1	films					750:754	Water chestnut starch nanocomposites films	713:754	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs)	713:813	Water chestnut starch nanocomposites films with varying concentrations of starch nanoparticles (SNPs) were synthesized by a solution casting method.
31247231	7	70	theme	composite	993:1001	arg1	films					1003:1007	nano starch composite films	981:1007	nano starch composite films	981:1007	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	7	71	theme	nano	981:984	arg1	films					1003:1007	nano starch composite films	981:1007	nano starch composite films	981:1007	The thickness, moisture content, water vapour transmission rate, water solubility, burst strength of native starch and nano starch composite films were evaluated.
31247231	9	72	from	increase	1320:1327	arg1	thickness					1332:1340	thickness	1332:1340	thickness	1332:1340	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	9	72	from	increase	1320:1327	arg1	strength					1352:1359	burst strength	1346:1359	burst strength	1346:1359	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	2	73	theme	acid	312:315	arg1	method					328:333	the acid hydrolysis method	308:333	the acid hydrolysis method having a yield of 27.5%	308:357	Nano starch was prepared by adopting the acid hydrolysis method having a yield of 27.5%.
31247231	4	74	theme	spherical	555:563	arg1	granules					565:572	spherical granules	555:572	spherical granules without cracks and smooth surface	555:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	9	75	theme	good	1535:1538	arg1	package					1540:1546	a good package	1533:1546	a good package	1533:1546	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	4	76	theme	smooth	593:598	arg1	surface					600:606	smooth surface	593:606	smooth surface	593:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	1	77	theme	present	130:136	arg1	study					138:142	the present study	126:142	the present study	126:142	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
31247231	9	78	theme	SNPs	1301:1304	arg1	incorporation					1284:1296	The incorporation	1280:1296	The incorporation of SNPs	1280:1304	The incorporation of SNPs results in an increase in thickness and burst strength while moisture content, water vapour transmission rate and solubility of films were decreased with the increase in the concentration of SNPs which is essential features of a good package.
31247231	0	79	theme	non-conventional	63:78	arg1	source					80:85	non-conventional source	63:85	non-conventional source	63:85	Development of starch nanoparticles based composite films from non-conventional source - Water chestnut (Trapa bispinosa).
31247231	4	80	theme	starch	474:479	arg1	shape					489:493	the shape	485:493	the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface	485:606	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	4	80	theme	starch	474:479	arg1	feature					448:454	The unique feature	437:454	The unique feature of water chestnut starch	437:479	The unique feature of water chestnut starch was the shape of starch granule that looked oval, ellipsoidal, mixed with spherical granules without cracks and smooth surface.
31247231	8	81	theme	vapour	1139:1144	arg1	4.678 × 10-3 ± 0.42 g-2 s-1					1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	81	theme	vapour	1139:1144	arg1	rate					1159:1162	water vapour transmission rate	1133:1162	water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1)	1133:1192	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	82	theme	water	1133:1137	arg1	4.678 × 10-3 ± 0.42 g-2 s-1					1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	4.678 × 10-3 ± 0.42 g-2 s-1	1165:1191	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	82	theme	water	1133:1137	arg1	rate					1159:1162	water vapour transmission rate	1133:1162	water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1)	1133:1192	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	83	theme	thickness	1072:1080	arg1	content					1109:1115	thickness (0.041 ± 0.07 mm) moisture content	1072:1115	thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%)	1072:1130	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	8	83	theme	thickness	1072:1080	arg1	%					1129:1129	4.17 ± 0.32%	1118:1129	4.17 ± 0.32%	1118:1129	The results showed that native starch film had thickness (0.041 ± 0.07 mm) moisture content (4.17 ± 0.32%), water vapour transmission rate (4.678 × 10-3 ± 0.42 g-2 s-1), water solubility (35.71 ± 0.17%) and burst strength (976.4 ± 12.47 g), respectively.
31247231	1	84	theme	various	217:223	arg1	properties					241:250	various physicochemical properties	217:250	various physicochemical properties	217:250	In the present study, starch was isolated from a non-conventional source (water chestnut) and various physicochemical properties were investigated.
30772408	0	0	theme	chitin/CM-chitosan	66:83	arg1	graft					102:106	a chitin/CM-chitosan artificial nerve graft	64:106	a chitin/CM-chitosan artificial nerve graft	64:106	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	4	1	with	cells	754:758	arg1	biocompatibility					783:798	good cell biocompatibility	773:798	good cell biocompatibility	773:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	3	2	theme	chitin-based	523:534	arg1	conduit					536:542	microporous chitin-based conduit	511:542	microporous chitin-based conduit	511:542	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	1	3	theme	biodegradability	339:354	arg1	properties					259:268	its excellent properties	245:268	its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	245:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	8	4	theme	nerve	1245:1249	arg1	grafts					1251:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	0	5	theme	nerve	96:100	arg1	graft					102:106	a chitin/CM-chitosan artificial nerve graft	64:106	a chitin/CM-chitosan artificial nerve graft	64:106	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	1	6	theme	excellent	249:257	arg1	properties					259:268	its excellent properties	245:268	its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	245:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	0	7	theme	artificial	85:94	arg1	graft					102:106	a chitin/CM-chitosan artificial nerve graft	64:106	a chitin/CM-chitosan artificial nerve graft	64:106	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	4	8	theme	cell	778:781	arg1	biocompatibility					783:798	good cell biocompatibility	773:798	good cell biocompatibility	773:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	4	9	theme	cells	754:758	arg1	proliferation					725:737	the proliferation	721:737	the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility	721:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	3	10	theme	chitosan-based	473:486	arg1	graft					494:498	a novel composite chitosan-based nerve graft	455:498	a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber	455:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	3	11	theme	CM-chitosan	557:567	arg1	fiber					569:573	internal CM-chitosan fiber	548:573	internal CM-chitosan fiber	548:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	3	12	from	defect	642:647	arg1	rats					655:658	SD rats	652:658	SD rats	652:658	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	4	13	theme	nerve	695:699	arg1	graft					701:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	3	14	theme	composite	463:471	arg1	graft					494:498	a novel composite chitosan-based nerve graft	455:498	a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber	455:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	6	15	theme	tissue	912:917	arg1	inflammation					919:930	tissue inflammation	912:930	tissue inflammation	912:930	No apparent toxicity was observed, and tissue inflammation was very slight after implantation, indicating favorable bio-safety of the nerve graft.
30772408	5	16	theme	nerve	836:840	arg1	graft					842:846	the artificial nerve graft	821:846	the artificial nerve graft	821:846	After implantation, the artificial nerve graft showed slow degradation.
30772408	4	17	theme	artificial	684:693	arg1	graft					701:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	0	18	theme	sciatic	4:10	arg1	regeneration					18:29	Rat sciatic nerve regeneration	0:29	Rat sciatic nerve regeneration across a 10-mm defect	0:51	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	7	19	theme	similar	1141:1147	arg1	effect					1149:1154	similar effect	1141:1154	similar effect	1141:1154	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	0	20	theme	Rat	0:2	arg1	regeneration					18:29	Rat sciatic nerve regeneration	0:29	Rat sciatic nerve regeneration across a 10-mm defect	0:51	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	6	21	theme	graft	1013:1017	arg1	bio-safety					989:998	favorable bio-safety	979:998	favorable bio-safety of the nerve graft	979:1017	No apparent toxicity was observed, and tissue inflammation was very slight after implantation, indicating favorable bio-safety of the nerve graft.
30772408	8	22	theme	nervous	1338:1344	arg1	injury					1346:1351	peripheral nervous injury	1327:1351	peripheral nervous injury	1327:1351	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	3	23	theme	bridge	606:611	arg1	nerve					621:625	bridge sciatic nerve	606:625	bridge sciatic nerve across a 10-mm defect in SD rats	606:658	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	8	24	theme	composite	1202:1210	arg1	grafts					1251:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	1	25	theme	low	273:275	arg1	toxicity					277:284	low toxicity	273:284	low toxicity	273:284	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	7	26	theme	neurons	1128:1134	arg1	restoration					1105:1115	restoration	1105:1115	restoration of damaged neurons with similar effect	1105:1154	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	4	27	theme	good	773:776	arg1	biocompatibility					783:798	good cell biocompatibility	773:798	good cell biocompatibility	773:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	2	28	theme	grade	429:433	arg1	chitosan					435:442	pharmaceutical grade chitosan	414:442	pharmaceutical grade chitosan	414:442	In this study, chitin and CM-chitosan were prepared from pharmaceutical grade chitosan.
30772408	0	29	theme	nerve	12:16	arg1	regeneration					18:29	Rat sciatic nerve regeneration	0:29	Rat sciatic nerve regeneration across a 10-mm defect	0:51	Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
30772408	1	30	theme	toxicity	277:284	arg1	properties					259:268	its excellent properties	245:268	its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	245:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	8	31	theme	chitin/CM-chitosan	1226:1243	arg1	grafts					1251:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	2	32	theme	pharmaceutical	414:427	arg1	chitosan					435:442	pharmaceutical grade chitosan	414:442	pharmaceutical grade chitosan	414:442	In this study, chitin and CM-chitosan were prepared from pharmaceutical grade chitosan.
30772408	4	33	theme	rat	742:744	arg1	RSC96					761:765	RSC96	761:765	RSC96	761:765	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	4	33	theme	rat	742:744	arg1	cells					754:758	rat Schwann cells	742:758	rat Schwann cells (RSC96) with good cell biocompatibility	742:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	7	34	with	neurons	1128:1134	arg1	effect					1149:1154	similar effect	1141:1154	similar effect	1141:1154	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	8	35	theme	favorable	1268:1276	arg1	biocompatibility					1278:1293	favorable biocompatibility	1268:1293	favorable biocompatibility	1268:1293	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	4	36	theme	chitin/CM-chitosan	665:682	arg1	graft					701:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft	661:705	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	8	37	theme	good	1299:1302	arg1	potential					1304:1312	good potential	1299:1312	good potential	1299:1312	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	1	38	theme	antibacterial	287:299	arg1	properties					301:310	antibacterial properties	287:310	antibacterial properties	287:310	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	7	39	theme	damaged	1120:1126	arg1	neurons					1128:1134	damaged neurons	1120:1134	damaged neurons with similar effect	1120:1154	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	7	40	theme	nerve	1067:1071	arg1	graft					1073:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	1	41	theme	peripheral	213:222	arg1	injury					230:235	peripheral nerve injury	213:235	peripheral nerve injury	213:235	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	1	42	theme	properties	301:310	arg1	properties					259:268	its excellent properties	245:268	its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	245:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	6	43	theme	nerve	1007:1011	arg1	graft					1013:1017	the nerve graft	1003:1017	the nerve graft	1003:1017	No apparent toxicity was observed, and tissue inflammation was very slight after implantation, indicating favorable bio-safety of the nerve graft.
30772408	3	44	theme	sciatic	613:619	arg1	nerve					621:625	bridge sciatic nerve	606:625	bridge sciatic nerve across a 10-mm defect in SD rats	606:658	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	4	45	theme	Schwann	746:752	arg1	RSC96					761:765	RSC96	761:765	RSC96	761:765	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	4	45	theme	Schwann	746:752	arg1	cells					754:758	rat Schwann cells	742:758	rat Schwann cells (RSC96) with good cell biocompatibility	742:798	The chitin/CM-chitosan artificial nerve graft could promote the proliferation of rat Schwann cells (RSC96) with good cell biocompatibility.
30772408	5	46	theme	artificial	825:834	arg1	graft					842:846	the artificial nerve graft	821:846	the artificial nerve graft	821:846	After implantation, the artificial nerve graft showed slow degradation.
30772408	6	47	theme	apparent	876:883	arg1	toxicity					885:892	No apparent toxicity	873:892	No apparent toxicity	873:892	No apparent toxicity was observed, and tissue inflammation was very slight after implantation, indicating favorable bio-safety of the nerve graft.
30772408	7	48	theme	chitin/CM-chitosan	1037:1054	arg1	graft					1073:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	3	49	theme	microporous	511:521	arg1	conduit					536:542	microporous chitin-based conduit	511:542	microporous chitin-based conduit	511:542	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	1	50	theme	high	313:316	arg1	biocompatibility					318:333	high biocompatibility	313:333	high biocompatibility	313:333	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	8	51	theme	biodegradable	1212:1224	arg1	grafts					1251:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	7	52	theme	artificial	1056:1065	arg1	graft					1073:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	the chitin/CM-chitosan artificial nerve graft	1033:1077	Furthermore, the chitin/CM-chitosan artificial nerve graft could effectively promote restoration of damaged neurons with similar effect compared to the autograft.
30772408	3	53	theme	nerve	488:492	arg1	graft					494:498	a novel composite chitosan-based nerve graft	455:498	a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber	455:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	1	54	theme	nerve	224:228	arg1	injury					230:235	peripheral nerve injury	213:235	peripheral nerve injury	213:235	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	3	55	theme	SD	652:653	arg1	rats					655:658	SD rats	652:658	SD rats	652:658	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	1	56	theme	natural	123:129	arg1	biopolymer					141:150	a natural bioactive biopolymer	121:150	a natural bioactive biopolymer	121:150	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	3	57	theme	novel	457:461	arg1	graft					494:498	a novel composite chitosan-based nerve graft	455:498	a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber	455:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	8	58	contain	possessed	1258:1266	arg2	potential					1304:1312	good potential	1299:1312	good potential	1299:1312	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	8	58	contain	possessed	1258:1266	arg2	biocompatibility					1278:1293	favorable biocompatibility	1268:1293	favorable biocompatibility	1268:1293	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	8	58	contain	possessed	1258:1266	arg1	grafts					1251:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	the composite biodegradable chitin/CM-chitosan nerve grafts	1198:1256	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	1	59	theme	conduit	191:197	arg1	guidance					182:189	guidance	182:189	guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	182:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	1	60	theme	bioactive	131:139	arg1	biopolymer					141:150	a natural bioactive biopolymer	121:150	a natural bioactive biopolymer	121:150	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	8	61	theme	peripheral	1327:1336	arg1	injury					1346:1351	peripheral nervous injury	1327:1351	peripheral nervous injury	1327:1351	In conclusion, the composite biodegradable chitin/CM-chitosan nerve grafts possessed favorable biocompatibility and good potential in repairing peripheral nervous injury.
30772408	1	62	theme	biocompatibility	318:333	arg1	properties					259:268	its excellent properties	245:268	its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability	245:354	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30772408	5	63	theme	slow	855:858	arg1	degradation					860:870	slow degradation	855:870	slow degradation	855:870	After implantation, the artificial nerve graft showed slow degradation.
30772408	3	64	theme	internal	548:555	arg1	fiber					569:573	internal CM-chitosan fiber	548:573	internal CM-chitosan fiber	548:573	Moreover, a novel composite chitosan-based nerve graft comprising microporous chitin-based conduit and internal CM-chitosan fiber was constructed and applied to bridge sciatic nerve across a 10-mm defect in SD rats.
30772408	6	65	theme	favorable	979:987	arg1	bio-safety					989:998	favorable bio-safety	979:998	favorable bio-safety of the nerve graft	979:1017	No apparent toxicity was observed, and tissue inflammation was very slight after implantation, indicating favorable bio-safety of the nerve graft.
30772408	1	66	theme	due	238:240	arg1	injury					230:235	peripheral nerve injury	213:235	peripheral nerve injury	213:235	Chitosan as a natural bioactive biopolymer has been commonly employed in guidance conduit for repairing peripheral nerve injury, due to its excellent properties of low toxicity, antibacterial properties, high biocompatibility and biodegradability.
30571946	1	0	theme	liquid	245:250	arg1	spectrometry					279:290	liquid chromatography tandem mass spectrometry	245:290	liquid chromatography tandem mass spectrometry using multiple reaction monitoring	245:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	2	1	theme	sulfate	363:369	arg1	levels					345:350	ng/mL levels	339:350	ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan	339:406	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
30571946	1	2	theme	chromatography	252:265	arg1	spectrometry					279:290	liquid chromatography tandem mass spectrometry	245:290	liquid chromatography tandem mass spectrometry using multiple reaction monitoring	245:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	0	3	from	Glycosaminoglycans	0:17	arg1	fluid					42:46	human cerebrospinal fluid	22:46	human cerebrospinal fluid	22:46	Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.
30571946	3	4	located	found	484:488	arg2	those					478:482	those	478:482	those	478:482	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	3	4	located	found	484:488	arg1	plasma					499:504	human plasma	493:504	human plasma	493:504	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	1	5	theme	tandem	267:272	arg1	spectrometry					279:290	liquid chromatography tandem mass spectrometry	245:290	liquid chromatography tandem mass spectrometry using multiple reaction monitoring	245:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	2	6	theme	ng/mL	339:343	arg1	levels					345:350	ng/mL levels	339:350	ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan	339:406	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
30571946	3	7	dep	amounts	413:419	arg1	The					409:411	The	409:411	The	409:411	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	3	8	theme	GAGs	459:462	arg1	GAGs					459:462	these GAGs	453:462	these GAGs	453:462	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	3	8	theme	GAGs	459:462	arg1	amounts					413:419	amounts	413:419	amounts	413:419	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	3	8	theme	GAGs	459:462	arg1	composition					438:448	disaccharide composition	425:448	disaccharide composition	425:448	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	1	9	theme	mass	274:277	arg1	spectrometry					279:290	liquid chromatography tandem mass spectrometry	245:290	liquid chromatography tandem mass spectrometry using multiple reaction monitoring	245:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	4	10	theme	nervous	604:610	arg1	system					612:617	the central nervous system	592:617	the central nervous system	592:617	This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
30571946	4	11	theme	biomarkers	565:574	arg1	discovery					552:560	the discovery	548:560	the discovery of biomarkers	548:574	This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
30571946	2	12	theme	hyaluronan	397:406	arg1	levels					345:350	ng/mL levels	339:350	ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan	339:406	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
30571946	0	13	theme	cerebrospinal	28:40	arg1	fluid					42:46	human cerebrospinal fluid	22:46	human cerebrospinal fluid	22:46	Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.
30571946	0	14	theme	human	22:26	arg1	fluid					42:46	human cerebrospinal fluid	22:46	human cerebrospinal fluid	22:46	Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.
30571946	1	15	theme	multiple	298:305	arg1	monitoring					316:325	multiple reaction monitoring	298:325	multiple reaction monitoring	298:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	3	16	theme	disaccharide	425:436	arg1	composition					438:448	disaccharide composition	425:448	disaccharide composition	425:448	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	1	17	theme	reaction	307:314	arg1	monitoring					316:325	multiple reaction monitoring	298:325	multiple reaction monitoring	298:325	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	1	18	theme	polysaccharide	206:219	arg1	lyases					221:226	polysaccharide lyases	206:226	polysaccharide lyases	206:226	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	0	19	theme	LC-MS/MS	62:69	arg1	MRM					71:73	LC-MS/MS MRM	62:73	LC-MS/MS MRM	62:73	Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.
30571946	4	20	theme	new	533:535	arg1	method					537:542	a new method	531:542	a new method for the discovery of biomarkers for diseases of the central nervous system	531:617	This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
30571946	2	21	theme	heparan	355:361	arg1	sulfate					363:369	heparan sulfate	355:369	heparan sulfate	355:369	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
30571946	1	22	theme	human	122:126	arg1	CSF					151:153	CSF	151:153	CSF	151:153	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	1	22	theme	human	122:126	arg1	fluid					144:148	human cerebral spinal fluid	122:148	human cerebral spinal fluid (CSF)	122:154	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	1	23	theme	cerebral	128:135	arg1	CSF					151:153	CSF	151:153	CSF	151:153	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	1	23	theme	cerebral	128:135	arg1	fluid					144:148	human cerebral spinal fluid	122:148	human cerebral spinal fluid (CSF)	122:154	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	4	24	theme	system	612:617	arg1	diseases					580:587	diseases	580:587	diseases of the central nervous system	580:617	This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
30571946	1	25	theme	spinal	137:142	arg1	CSF					151:153	CSF	151:153	CSF	151:153	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	1	25	theme	spinal	137:142	arg1	fluid					144:148	human cerebral spinal fluid	122:148	human cerebral spinal fluid (CSF)	122:154	Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring.
30571946	3	26	theme	human	493:497	arg1	plasma					499:504	human plasma	493:504	human plasma	493:504	The amounts and disaccharide composition of these GAGs differed from those found in human plasma.
30571946	2	27	theme	sulfates	384:391	arg1	levels					345:350	ng/mL levels	339:350	ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan	339:406	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
30571946	4	28	theme	central	596:602	arg1	system					612:617	the central nervous system	592:617	the central nervous system	592:617	This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.
30571946	2	29	theme	chondroitin	372:382	arg1	sulfates					384:391	chondroitin sulfates	372:391	chondroitin sulfates	372:391	CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan.
31732088	3	0	attach	derived	530:536	arg2	product					522:528	a product	520:528	a product derived from wheat flour production	520:564	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	0	attach	derived	530:536	arg2	bioprocessing					486:498	bioprocessing	486:498	bioprocessing of wheat middlings	486:517	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	0	attach	derived	530:536	arg1	production					555:564	wheat flour production	543:564	wheat flour production	543:564	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	1	1	theme	health-promoting	188:203	arg1	effect					205:210	a health-promoting effect	186:210	a health-promoting effect	186:210	Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect.
31732088	5	2	theme	highest	845:851	arg1	70.31 U/mL					860:869	70.31 U/mL	860:869	70.31 U/mL	860:869	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	5	2	theme	highest	845:851	arg1	value					853:857	the highest value	841:857	the highest value (70.31 U/mL)	841:870	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	7	3	theme	commercial	1126:1135	arg1	LA-5					1173:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	5	4	theme	bacterial	794:802	arg1	growth					804:809	the 72 h bacterial growth	785:809	the 72 h bacterial growth	785:809	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	6	5	theme	different	983:991	arg1	times					993:997	different times	983:997	different times	983:997	XOS profile and concentration varied considerably between control and bioprocessed samples and among these at different times.
31732088	6	6	theme	XOS	873:875	arg1	profile					877:883	XOS profile	873:883	XOS profile	873:883	XOS profile and concentration varied considerably between control and bioprocessed samples and among these at different times.
31732088	4	7	theme	enzymatic	635:643	arg1	activities					659:668	the enzymatic and prebiotic activities	631:668	the enzymatic and prebiotic activities of resulting B. subtilis cultures	631:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	8	8	theme	xylanolytic	1321:1331	arg1	enzymes					1333:1339	xylanolytic enzymes	1321:1339	xylanolytic enzymes	1321:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	1	9	theme	Prebiotic	90:98	arg1	substrates					114:123	substrates	114:123	substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect	114:210	Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect.
31732088	1	9	theme	Prebiotic	90:98	arg1	compounds					100:108	Prebiotic compounds	90:108	Prebiotic compounds	90:108	Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect.
31732088	8	10	theme	good	1306:1309	arg1	source					1311:1316	a good source	1304:1316	a good source of xylanolytic enzymes	1304:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	10	theme	good	1306:1309	arg1	FTC01					1284:1288	FTC01	1284:1288	FTC01	1284:1288	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	11	theme	prebiotics	1248:1257	arg1	production					1234:1243	production	1234:1243	production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes	1234:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	2	12	located	found	383:387	arg1	cereals					392:398	cereals	392:398	cereals	392:398	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	12	located	found	383:387	arg2	polysaccharides					367:381	polysaccharides	367:381	polysaccharides found in cereals	367:398	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	12	located	found	383:387	arg2	arabinoxylans					342:354	arabinoxylans	342:354	arabinoxylans	342:354	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	3	13	theme	wheat	503:507	arg1	middlings					509:517	wheat middlings	503:517	wheat middlings	503:517	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	0	14	from	middlings	43:51	arg1	production					21:30	Xylooligosaccharides production	0:30	Xylooligosaccharides production from wheat middlings	0:51	Xylooligosaccharides production from wheat middlings bioprocessed with Bacillus subtilis.
31732088	7	15	theme	acidophilus	1161:1171	arg1	LA-5					1173:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	8	16	dep	B.	1272:1273	arg1	appears					1290:1296	appears	1290:1296	appears to be a good source of xylanolytic enzymes	1290:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	16	dep	B.	1272:1273	arg1	subtilis					1275:1282	B. subtilis FTC01 appears to be a good source of xylanolytic enzymes	1272:1339	B. subtilis FTC01 appears to be a good source of xylanolytic enzymes	1272:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	0	17	theme	Xylooligosaccharides	0:19	arg1	production					21:30	Xylooligosaccharides production	0:30	Xylooligosaccharides production from wheat middlings	0:51	Xylooligosaccharides production from wheat middlings bioprocessed with Bacillus subtilis.
31732088	7	18	theme	prebiotic	1008:1016	arg1	score					1027:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	0	19	theme	wheat	37:41	arg1	middlings					43:51	wheat middlings	37:51	wheat middlings	37:51	Xylooligosaccharides production from wheat middlings bioprocessed with Bacillus subtilis.
31732088	7	20	theme	probiotic	1137:1145	arg1	LA-5					1173:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	the commercial probiotic Lactobacillus acidophilus LA-5	1122:1176	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	7	21	theme	activity	1018:1025	arg1	score					1027:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	8	22	theme	enzymes	1333:1339	arg1	source					1311:1316	a good source	1304:1316	a good source of xylanolytic enzymes	1304:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	22	theme	enzymes	1333:1339	arg1	FTC01					1284:1288	FTC01	1284:1288	FTC01	1284:1288	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	4	23	theme	XOS	623:625	arg1	composition					608:618	The composition	604:618	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures	604:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	2	24	theme	prebiotic	226:234	arg1	carbohydrates					236:248	some prebiotic carbohydrates	221:248	some prebiotic carbohydrates	221:248	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	4	25	theme	B.	683:684	arg1	cultures					695:702	resulting B. subtilis cultures	673:702	resulting B. subtilis cultures	673:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	3	26	theme	flour	549:553	arg1	production					555:564	wheat flour production	543:564	wheat flour production	543:564	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	8	27	theme	Wheat	1179:1183	arg1	substrate					1220:1228	a promising substrate	1208:1228	a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes	1208:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	27	theme	Wheat	1179:1183	arg1	middlings					1185:1193	Wheat middlings	1179:1193	Wheat middlings	1179:1193	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	6	28	theme	bioprocessed	943:954	arg1	samples					956:962	bioprocessed samples	943:962	bioprocessed samples	943:962	XOS profile and concentration varied considerably between control and bioprocessed samples and among these at different times.
31732088	3	29	theme	middlings	509:517	arg1	bioprocessing					486:498	bioprocessing	486:498	bioprocessing of wheat middlings	486:517	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	29	theme	middlings	509:517	arg1	product					522:528	a product	520:528	a product derived from wheat flour production	520:564	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	30	theme	XOS	475:477	arg1	production					437:446	the production	433:446	the production of xylanolytic enzymes and XOS	433:477	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	5	31	theme	72 h	789:792	arg1	growth					804:809	the 72 h bacterial growth	785:809	the 72 h bacterial growth	785:809	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	4	32	theme	cultures	695:702	arg1	activities					659:668	the enzymatic and prebiotic activities	631:668	the enzymatic and prebiotic activities of resulting B. subtilis cultures	631:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	4	32	theme	cultures	695:702	arg1	XOS					623:625	XOS	623:625	XOS	623:625	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	3	33	theme	xylanolytic	451:461	arg1	enzymes					463:469	xylanolytic enzymes	451:469	xylanolytic enzymes	451:469	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	34	theme	wheat	543:547	arg1	production					555:564	wheat flour production	543:564	wheat flour production	543:564	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	3	35	theme	enzymes	463:469	arg1	production					437:446	the production	433:446	the production of xylanolytic enzymes and XOS	433:477	This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis.
31732088	5	36	theme	xylanolytic	736:746	arg1	enzymes					748:754	xylanolytic enzymes	736:754	xylanolytic enzymes	736:754	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	4	37	dep	B.	683:684	arg1	subtilis					686:693	subtilis	686:693	subtilis	686:693	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	1	38	theme	beneficial	152:161	arg1	microbiota					167:176	beneficial gut microbiota	152:176	beneficial gut microbiota causing a health-promoting effect	152:210	Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect.
31732088	2	39	attach	derived	329:335	arg2	xylooligosaccharides					277:296	xylooligosaccharides	277:296	xylooligosaccharides (XOS)	277:302	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	39	attach	derived	329:335	arg2	prebiotics					318:327	important prebiotics	308:327	important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals	308:398	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	39	attach	derived	329:335	arg1	arabinoxylans					342:354	arabinoxylans	342:354	arabinoxylans	342:354	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	39	attach	derived	329:335	arg1	polysaccharides					367:381	polysaccharides	367:381	polysaccharides found in cereals	367:398	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	5	40	theme	enzymes	748:754	arg1	activity					724:731	The activity	720:731	The activity of xylanolytic enzymes	720:754	The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL).
31732088	2	41	theme	important	308:316	arg1	xylooligosaccharides					277:296	xylooligosaccharides	277:296	xylooligosaccharides (XOS)	277:302	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	2	41	theme	important	308:316	arg1	prebiotics					318:327	important prebiotics	308:327	important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals	308:398	Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals.
31732088	1	42	theme	gut	163:165	arg1	microbiota					167:176	beneficial gut microbiota	152:176	beneficial gut microbiota causing a health-promoting effect	152:210	Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect.
31732088	7	43	theme	Maximum	1000:1006	arg1	score					1027:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score	1000:1031	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	4	44	theme	activities	659:668	arg1	composition					608:618	The composition	604:618	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures	604:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	7	45	theme	bioprocessed	1065:1076	arg1	samples					1078:1084	bioprocessed samples	1065:1084	bioprocessed samples	1065:1084	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	4	46	theme	prebiotic	649:657	arg1	activities					659:668	the enzymatic and prebiotic activities	631:668	the enzymatic and prebiotic activities of resulting B. subtilis cultures	631:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
31732088	8	47	theme	promising	1210:1218	arg1	substrate					1220:1228	a promising substrate	1208:1228	a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes	1208:1339	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	8	47	theme	promising	1210:1218	arg1	middlings					1185:1193	Wheat middlings	1179:1193	Wheat middlings	1179:1193	Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
31732088	7	48	dep	24 h	1051:1054	arg1	1.73					1087:1090	1.73	1087:1090	1.73	1087:1090	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	7	48	dep	24 h	1051:1054	arg1	samples					1078:1084	bioprocessed samples	1065:1084	bioprocessed samples	1065:1084	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	7	48	dep	24 h	1051:1054	arg1	the					1047:1049	the	1047:1049	the	1047:1049	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	7	48	dep	24 h	1051:1054	arg1	1.61					1096:1099	1.61	1096:1099	1.61	1096:1099	Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5.
31732088	4	49	theme	resulting	673:681	arg1	cultures					695:702	resulting B. subtilis cultures	673:702	resulting B. subtilis cultures	673:702	The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated.
30207990	4	0	dep	transform	537:545	arg1	infrared					547:554	infrared	547:554	transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis	537:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	5	1	theme	Zr/Ti/CHT	677:685	arg1	catalyst					697:704	the Zr/Ti/CHT composite catalyst	673:704	the Zr/Ti/CHT composite catalyst	673:704	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	8	2	theme	0.009	1054:1058	arg1	values					1030:1035	The kapp values	1021:1035	The kapp values	1021:1035	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	8	2	theme	0.009	1054:1058	arg1	min-1					1085:1089	0.009, 0.0013, 0.012 and 0.014 min-1	1054:1089	min-1	1085:1089	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	1	3	theme	composite	187:195	arg1	catalysts					197:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	5	4	theme	phase	748:752	arg1	crystallinity					718:730	high crystallinity	713:730	high crystallinity of anatase TiO2 phase	713:752	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	4	5	dep	Fourier	529:535	arg1	transform					537:545	transform	537:545	transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis	537:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	5	6	theme	composite	687:695	arg1	catalyst					697:704	the Zr/Ti/CHT composite catalyst	673:704	the Zr/Ti/CHT composite catalyst	673:704	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	6	7	theme	light	888:892	arg1	irradiation					894:904	visible light irradiation	880:904	visible light irradiation	880:904	Photocatalytic experiments showed that adding CHT into the catalyst structure increased the photocatalytic degradation under visible light irradiation.
30207990	9	8	theme	fifth	1272:1276	arg1	cycle					1278:1282	the fifth cycle	1268:1282	the fifth cycle	1268:1282	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	8	9	theme	0.014	1079:1083	arg1	values					1030:1035	The kapp values	1021:1035	The kapp values	1021:1035	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	8	9	theme	0.014	1079:1083	arg1	min-1					1085:1089	0.009, 0.0013, 0.012 and 0.014 min-1	1054:1089	min-1	1085:1089	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	6	10	theme	visible	880:886	arg1	irradiation					894:904	visible light irradiation	880:904	visible light irradiation	880:904	Photocatalytic experiments showed that adding CHT into the catalyst structure increased the photocatalytic degradation under visible light irradiation.
30207990	9	11	theme	first	1186:1190	arg1	cycle					1192:1196	the first cycle	1182:1196	the first cycle	1182:1196	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	4	12	theme	photoelectron	609:621	arg1	analysis					636:643	X-ray photoelectron spectroscopy analysis	603:643	X-ray photoelectron spectroscopy analysis	603:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	9	13	theme	II	1205:1206	arg1	degradation					1212:1222	Orange II dye degradation	1198:1222	Orange II dye degradation	1198:1222	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	6	14	theme	Photocatalytic	755:768	arg1	experiments					770:780	Photocatalytic experiments	755:780	Photocatalytic experiments	755:780	Photocatalytic experiments showed that adding CHT into the catalyst structure increased the photocatalytic degradation under visible light irradiation.
30207990	4	15	theme	X-ray	603:607	arg1	analysis					636:643	X-ray photoelectron spectroscopy analysis	603:643	X-ray photoelectron spectroscopy analysis	603:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	7	16	theme	reaction	929:936	arg1	kapp					953:956	kapp	953:956	kapp	953:956	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	7	16	theme	reaction	929:936	arg1	constant					943:950	the first-order reaction rate constant	913:950	the first-order reaction rate constant	913:950	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	5	17	theme	XRD	646:648	arg1	analysis					650:657	XRD analysis	646:657	XRD analysis	646:657	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	7	18	theme	rate	938:941	arg1	kapp					953:956	kapp	953:956	kapp	953:956	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	7	18	theme	rate	938:941	arg1	constant					943:950	the first-order reaction rate constant	913:950	the first-order reaction rate constant	913:950	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	0	19	theme	Orange	132:137	arg1	dye					142:144	Orange II dye	132:144	Orange II dye	132:144	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	4	20	theme	X-ray	504:508	arg1	XRD					523:525	XRD	523:525	XRD	523:525	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	4	20	theme	X-ray	504:508	arg1	Fourier					529:535	Fourier	529:535	Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis	529:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	4	20	theme	X-ray	504:508	arg1	diffraction					510:520	X-ray diffraction	504:520	X-ray diffraction (XRD)	504:526	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	6	21	theme	photocatalytic	847:860	arg1	degradation					862:872	the photocatalytic degradation	843:872	the photocatalytic degradation	843:872	Photocatalytic experiments showed that adding CHT into the catalyst structure increased the photocatalytic degradation under visible light irradiation.
30207990	4	22	theme	spectroscopy	623:634	arg1	analysis					636:643	X-ray photoelectron spectroscopy analysis	603:643	X-ray photoelectron spectroscopy analysis	603:643	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	3	23	dep	Zr/Ti	422:426	arg1	catalysts					447:455	catalysts	447:455	catalysts	447:455	The results were compared with Zr/Ti, Zr/CHT and Ti/CHT catalysts.
30207990	2	24	theme	Orange	343:348	arg1	dye					353:355	Orange II dye	343:355	Orange II dye	343:355	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30207990	7	25	theme	first-order	917:927	arg1	kapp					953:956	kapp	953:956	kapp	953:956	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	7	25	theme	first-order	917:927	arg1	constant					943:950	the first-order reaction rate constant	913:950	the first-order reaction rate constant	913:950	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	7	26	theme	Langmuir-Hinshelwood	984:1003	arg1	equation					1011:1018	the Langmuir-Hinshelwood (L-H) equation	980:1018	the Langmuir-Hinshelwood (L-H) equation	980:1018	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	2	27	from	%	284:284	arg1	degradation					328:338	the photocatalytic degradation	309:338	the photocatalytic degradation of Orange II dye	309:355	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30207990	1	28	theme	sol-gel	227:233	arg1	method					235:240	sol-gel method	227:240	sol-gel method using different chitosan amounts (5-20 wt	227:282	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	0	29	theme	TiO2/chitosan	24:36	arg1	catalysts					48:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	10	30	theme	dye	1374:1376	arg1	degradation					1378:1388	Orange II dye degradation	1364:1388	Orange II dye degradation	1364:1388	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	5	31	theme	TiO2	743:746	arg1	phase					748:752	anatase TiO2 phase	735:752	anatase TiO2 phase	735:752	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	9	32	theme	dye	1208:1210	arg1	degradation					1212:1222	Orange II dye degradation	1198:1222	Orange II dye degradation	1198:1222	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	9	33	theme	Orange	1198:1203	arg1	degradation					1212:1222	Orange II dye degradation	1198:1222	Orange II dye degradation	1198:1222	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	10	34	theme	Orange	1364:1369	arg1	degradation					1378:1388	Orange II dye degradation	1364:1388	Orange II dye degradation	1364:1388	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	0	35	theme	Zr-doped	15:22	arg1	catalysts					48:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	4	36	theme	electron	579:586	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy	570:597	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	0	37	theme	dye	142:144	arg1	degradation					117:127	the degradation	113:127	the degradation of Orange II dye	113:144	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	2	38	theme	photocatalytic	313:326	arg1	degradation					328:338	the photocatalytic degradation	309:338	the photocatalytic degradation of Orange II dye	309:355	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30207990	0	39	theme	catalysts	48:56	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.	0:145	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	4	40	theme	scanning	570:577	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy	570:597	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	1	41	theme	different	248:256	arg1	amounts					267:273	different chitosan amounts	248:273	different chitosan amounts (5-20 wt	248:282	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	0	42	theme	composite	38:46	arg1	catalysts					48:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Zr-doped TiO2/chitosan composite catalysts	15:56	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	1	43	dep	amounts	267:273	arg1	wt					281:282	5-20 wt	276:282	different chitosan amounts (5-20 wt	248:282	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	6	44	theme	catalyst	814:821	arg1	structure					823:831	the catalyst structure	810:831	the catalyst structure	810:831	Photocatalytic experiments showed that adding CHT into the catalyst structure increased the photocatalytic degradation under visible light irradiation.
30207990	1	45	theme	chitosan	258:265	arg1	amounts					267:273	different chitosan amounts	248:273	different chitosan amounts (5-20 wt	248:282	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	0	46	theme	enhanced	63:70	arg1	photoactivity					95:107	enhanced visible-light-mediated photoactivity	63:107	enhanced visible-light-mediated photoactivity for the degradation of Orange II dye	63:144	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	5	47	theme	high	713:716	arg1	crystallinity					718:730	high crystallinity	713:730	high crystallinity of anatase TiO2 phase	713:752	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	4	48	theme	composite	462:470	arg1	catalysts					472:480	The composite catalysts	458:480	The composite catalysts	458:480	The composite catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy analysis.
30207990	9	49	theme	stability	1159:1167	arg1	tests					1169:1173	stability tests	1159:1173	stability tests	1159:1173	According to stability tests, after the first cycle Orange II dye degradation was found to be 95%, while it was 37% after the fifth cycle.
30207990	2	50	from	activity	297:304	arg1	degradation					328:338	the photocatalytic degradation	309:338	the photocatalytic degradation of Orange II dye	309:355	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30207990	10	51	theme	II	1371:1372	arg1	degradation					1378:1388	Orange II dye degradation	1364:1388	Orange II dye degradation	1364:1388	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	7	52	theme	L-H	1006:1008	arg1	equation					1011:1018	the Langmuir-Hinshelwood (L-H) equation	980:1018	the Langmuir-Hinshelwood (L-H) equation	980:1018	Also, the first-order reaction rate constant, kapp, was calculated using the Langmuir-Hinshelwood (L-H) equation.
30207990	0	53	with	Fabrication	0:10	arg1	photoactivity					95:107	enhanced visible-light-mediated photoactivity	63:107	enhanced visible-light-mediated photoactivity for the degradation of Orange II dye	63:144	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	1	54	theme	Zirconium/titanium/chitosan	147:173	arg1	catalysts					197:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	5	55	theme	anatase	735:741	arg1	phase					748:752	anatase TiO2 phase	735:752	anatase TiO2 phase	735:752	XRD analysis revealed that the Zr/Ti/CHT composite catalyst showed high crystallinity of anatase TiO2 phase.
30207990	10	56	theme	composite	1313:1321	arg1	catalyst					1323:1330	the composite catalyst	1309:1330	the composite catalyst	1309:1330	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	2	57	theme	dye	353:355	arg1	degradation					328:338	the photocatalytic degradation	309:338	the photocatalytic degradation of Orange II dye	309:355	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30207990	1	58	theme	Zr/Ti/CHT	176:184	arg1	catalysts					197:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts	147:205	Zirconium/titanium/chitosan (Zr/Ti/CHT) composite catalysts were synthesized by sol-gel method using different chitosan amounts (5-20 wt.
30207990	10	59	used	used	1341:1344	arg2	catalyst					1323:1330	the composite catalyst	1309:1330	the composite catalyst	1309:1330	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	10	60	theme	several	1346:1352	arg1	times					1354:1358	several times	1346:1358	several times	1346:1358	The results showed that the composite catalyst could be used several times for Orange II dye degradation.
30207990	0	61	theme	visible-light-mediated	72:93	arg1	photoactivity					95:107	enhanced visible-light-mediated photoactivity	63:107	enhanced visible-light-mediated photoactivity for the degradation of Orange II dye	63:144	Fabrication of Zr-doped TiO2/chitosan composite catalysts with enhanced visible-light-mediated photoactivity for the degradation of Orange II dye.
30207990	8	62	theme	kapp	1025:1028	arg1	values					1030:1035	The kapp values	1021:1035	The kapp values	1021:1035	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	8	62	theme	kapp	1025:1028	arg1	min-1					1085:1089	0.009, 0.0013, 0.012 and 0.014 min-1	1054:1089	min-1	1085:1089	The kapp values were found to be 0.009, 0.0013, 0.012 and 0.014 min-1 for Zr/Ti, Zr/CHT, Ti/CHT and Zr/Ti/CHT, respectively.
30207990	2	63	theme	first	379:383	arg1	time					385:388	the first time	375:388	the first time	375:388	%) and their activity in the photocatalytic degradation of Orange II dye was evaluated for the first time.
30213572	0	0	theme	silver	129:134	arg1	nanoparticles					136:148	silver nanoparticles	129:148	silver nanoparticles	129:148	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	9	1	theme	food	1228:1231	arg1	film					1208:1211	new nanocomposite film	1190:1211	new nanocomposite film	1190:1211	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	9	1	theme	food	1228:1231	arg1	packaging					1240:1248	food active packaging	1228:1248	food active packaging	1228:1248	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	1	2	theme	methylcellulose/Beeswax	222:244	arg1	AgNPs					279:283	AgNPs	279:283	AgNPs	279:283	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	1	2	theme	methylcellulose/Beeswax	222:244	arg1	nanoparticles					264:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles	197:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	197:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	8	3	theme	pathogen	1104:1111	arg1	bacteria					1113:1120	tested pathogen bacteria	1097:1120	tested pathogen bacteria	1097:1120	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	1	4	theme	reinforced	246:255	arg1	AgNPs					279:283	AgNPs	279:283	AgNPs	279:283	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	1	4	theme	reinforced	246:255	arg1	nanoparticles					264:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles	197:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	197:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	9	5	theme	new	1190:1192	arg1	film					1208:1211	new nanocomposite film	1190:1211	new nanocomposite film	1190:1211	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	9	5	theme	new	1190:1192	arg1	packaging					1240:1248	food active packaging	1228:1248	food active packaging	1228:1248	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	7	6	with	incorporation	990:1002	arg1	AgNPs					1009:1013	AgNPs	1009:1013	AgNPs	1009:1013	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	8	7	theme	tested	1097:1102	arg1	bacteria					1113:1120	tested pathogen bacteria	1097:1120	tested pathogen bacteria	1097:1120	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	6	8	dep	transform	839:847	arg1	infrared					849:856	infrared	849:856	transform infrared	839:856	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	5	9	theme	apparent	676:683	arg1	color					685:689	the apparent color	672:689	the apparent color of bio-composite film	672:711	The use of AgNPs influenced the apparent color of bio-composite film.
30213572	7	10	theme	film	954:957	arg1	stability					941:949	the thermal stability	929:949	the thermal stability of film	929:957	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	1	11	theme	trinary	161:167	arg1	film					183:186	The novel trinary bio-composite film	151:186	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	151:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	9	12	theme	antimicrobial	1155:1167	arg1	results					1169:1175	The antimicrobial results	1151:1175	The antimicrobial results	1151:1175	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	1	13	theme	silver	257:262	arg1	AgNPs					279:283	AgNPs	279:283	AgNPs	279:283	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	1	13	theme	silver	257:262	arg1	nanoparticles					264:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles	197:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	197:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	4	14	theme	vapor	546:550	arg1	permeability					552:563	water vapor permeability	540:563	water vapor permeability	540:563	However, water vapor permeability was improved by addition of the nanoparticles (4.57-2.16 × 10-13 g m/m2 s Pa).
30213572	5	15	theme	bio-composite	694:706	arg1	film					708:711	bio-composite film	694:711	bio-composite film	694:711	The use of AgNPs influenced the apparent color of bio-composite film.
30213572	8	16	theme	contact	1129:1135	arg1	zone					1145:1148	the contact surface zone	1125:1148	the contact surface zone	1125:1148	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	4	17	theme	water	540:544	arg1	permeability					552:563	water vapor permeability	540:563	water vapor permeability	540:563	However, water vapor permeability was improved by addition of the nanoparticles (4.57-2.16 × 10-13 g m/m2 s Pa).
30213572	5	18	theme	film	708:711	arg1	color					685:689	the apparent color	672:689	the apparent color of bio-composite film	672:711	The use of AgNPs influenced the apparent color of bio-composite film.
30213572	6	19	theme	films	770:774	arg1	topography					752:761	topography	752:761	topography	752:761	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	6	19	theme	films	770:774	arg1	structure					738:746	The microscopic surface structure	714:746	The microscopic surface structure	714:746	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	3	20	theme	composite	479:487	arg1	strength					497:504	the composite tensile strength	475:504	the composite tensile strength from 33.64 to 16.12 MPa	475:528	It was discovered that AgNPs reduced the composite tensile strength from 33.64 to 16.12 MPa.
30213572	0	21	theme	Physico-mechanical	0:17	arg1	properties					37:46	Physico-mechanical and antimicrobial properties	0:46	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films	0:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	7	22	theme	analysis	900:907	arg1	results					909:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	3	23	theme	tensile	489:495	arg1	strength					497:504	the composite tensile strength	475:504	the composite tensile strength from 33.64 to 16.12 MPa	475:528	It was discovered that AgNPs reduced the composite tensile strength from 33.64 to 16.12 MPa.
30213572	5	24	theme	AgNPs	655:659	arg1	use					648:650	The use	644:650	The use of AgNPs	644:659	The use of AgNPs influenced the apparent color of bio-composite film.
30213572	2	25	from	effect	329:334	arg1	properties					404:413	some physico-mechanical and antimicrobial properties	362:413	some physico-mechanical and antimicrobial properties of bio-composite film	362:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	9	26	used	used	1220:1223	arg2	packaging					1240:1248	food active packaging	1228:1248	food active packaging	1228:1248	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	9	26	used	used	1220:1223	arg2	film					1208:1211	new nanocomposite film	1190:1211	new nanocomposite film	1190:1211	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	0	27	theme	antimicrobial	23:35	arg1	properties					37:46	Physico-mechanical and antimicrobial properties	0:46	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films	0:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	7	28	theme	mechanical	881:890	arg1	results					909:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	1	29	theme	novel	155:159	arg1	film					183:186	The novel trinary bio-composite film	151:186	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	151:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	9	30	theme	active	1233:1238	arg1	film					1208:1211	new nanocomposite film	1190:1211	new nanocomposite film	1190:1211	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	9	30	theme	active	1233:1238	arg1	packaging					1240:1248	food active packaging	1228:1248	food active packaging	1228:1248	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	8	31	from	activity	1080:1087	arg1	zone					1145:1148	the contact surface zone	1125:1148	the contact surface zone	1125:1148	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	0	32	theme	tragacanth/hydroxypropyl	51:74	arg1	films					107:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	7	33	theme	thermal	892:898	arg1	results					909:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	8	34	theme	nano-composite	1025:1038	arg1	films					1040:1044	nano-composite films	1025:1044	nano-composite films	1025:1044	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	8	35	theme	strong	1059:1064	arg1	activity					1080:1087	strong antibacterial activity	1059:1087	strong antibacterial activity against tested pathogen bacteria in the contact surface zone	1059:1148	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	3	36	from	33.64	511:515	arg1	strength					497:504	the composite tensile strength	475:504	the composite tensile strength from 33.64 to 16.12 MPa	475:528	It was discovered that AgNPs reduced the composite tensile strength from 33.64 to 16.12 MPa.
30213572	0	37	theme	edible	100:105	arg1	films					107:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	7	38	theme	thermal	933:939	arg1	stability					941:949	the thermal stability	929:949	the thermal stability of film	929:957	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30213572	2	39	theme	antimicrobial	390:402	arg1	properties					404:413	some physico-mechanical and antimicrobial properties	362:413	some physico-mechanical and antimicrobial properties of bio-composite film	362:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	0	40	theme	methylcellulose/beeswax	76:98	arg1	films					107:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	tragacanth/hydroxypropyl methylcellulose/beeswax edible films	51:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	4	41	theme	nanoparticles	597:609	arg1	addition					581:588	addition	581:588	addition of the nanoparticles (4.57-2.16 × 10-13 g m/m2 s Pa)	581:641	However, water vapor permeability was improved by addition of the nanoparticles (4.57-2.16 × 10-13 g m/m2 s Pa).
30213572	1	42	theme	bio-composite	169:181	arg1	film					183:186	The novel trinary bio-composite film	151:186	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	151:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	8	43	theme	surface	1137:1143	arg1	zone					1145:1148	the contact surface zone	1125:1148	the contact surface zone	1125:1148	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	2	44	theme	physico-mechanical	367:384	arg1	properties					404:413	some physico-mechanical and antimicrobial properties	362:413	some physico-mechanical and antimicrobial properties of bio-composite film	362:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	6	45	theme	surface	730:736	arg1	structure					738:746	The microscopic surface structure	714:746	The microscopic surface structure	714:746	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	2	46	theme	AgNPs	339:343	arg1	effect					329:334	the effect	325:334	the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film	325:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	8	47	theme	antibacterial	1066:1078	arg1	activity					1080:1087	strong antibacterial activity	1059:1087	strong antibacterial activity against tested pathogen bacteria in the contact surface zone	1059:1148	Finally, nano-composite films demonstrated strong antibacterial activity against tested pathogen bacteria in the contact surface zone.
30213572	1	48	theme	Tragacanth/Hydroxypropyl	197:220	arg1	AgNPs					279:283	AgNPs	279:283	AgNPs	279:283	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	1	48	theme	Tragacanth/Hydroxypropyl	197:220	arg1	nanoparticles					264:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles	197:276	Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs)	197:284	The novel trinary bio-composite film based on Tragacanth/Hydroxypropyl methylcellulose/Beeswax reinforced silver nanoparticles (AgNPs) was developed.
30213572	6	49	theme	microscopic	718:728	arg1	structure					738:746	The microscopic surface structure	714:746	The microscopic surface structure	714:746	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	9	50	theme	nanocomposite	1194:1206	arg1	film					1208:1211	new nanocomposite film	1190:1211	new nanocomposite film	1190:1211	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	9	50	theme	nanocomposite	1194:1206	arg1	packaging					1240:1248	food active packaging	1228:1248	food active packaging	1228:1248	The antimicrobial results suggest that new nanocomposite film may be used as food active packaging.
30213572	6	51	theme	electron	807:814	arg1	microscopy					816:825	scanning electron microscopy	798:825	scanning electron microscopy	798:825	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	2	52	theme	film	432:435	arg1	properties					404:413	some physico-mechanical and antimicrobial properties	362:413	some physico-mechanical and antimicrobial properties of bio-composite film	362:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	0	53	theme	films	107:111	arg1	properties					37:46	Physico-mechanical and antimicrobial properties	0:46	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films	0:111	Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles.
30213572	6	54	theme	scanning	798:805	arg1	microscopy					816:825	scanning electron microscopy	798:825	scanning electron microscopy	798:825	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	2	55	theme	bio-composite	418:430	arg1	film					432:435	bio-composite film	418:435	bio-composite film	418:435	This study investigated the effect of AgNPs (2, 4 and 8%) on some physico-mechanical and antimicrobial properties of bio-composite film.
30213572	6	56	dep	Fourier	831:837	arg1	transform					839:847	transform	839:847	transform infrared	839:856	The microscopic surface structure and topography of the films were also examined by scanning electron microscopy and Fourier transform infrared, respectively.
30213572	7	57	theme	Dynamic	873:879	arg1	results					909:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results	873:915	Dynamic mechanical thermal analysis results showed that the thermal stability of film was slightly decreased through incorporation with AgNPs.
30074061	0	0	theme	cross	75:79	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	4	1	theme	enzyme	683:688	arg1	reactor					699:705	the enzyme membrane reactor	679:705	the enzyme membrane reactor	679:705	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	0	2	from	production	11:20	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	0	2	from	production	11:20	arg1	pulp					65:68	sugar beet pulp	54:68	sugar beet pulp	54:68	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	6	3	theme	pectic	1109:1114	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	3	4	theme	filtration	620:629	arg1	suitability					631:641	filtration suitability	620:641	filtration suitability	620:641	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	1	5	theme	potential	237:245	arg1	prebiotics					247:256	potential prebiotics	237:256	potential prebiotics	237:256	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	0	6	theme	continuous	86:95	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	3	7	theme	Preliminary	495:505	arg1	experiments					507:517	Preliminary experiments	495:517	Preliminary experiments in batch and semi-continuous setups	495:553	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	3	8	from	experiments	507:517	arg1	setups					548:553	batch and semi-continuous setups	522:553	batch and semi-continuous setups	522:553	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	1	9	theme	prebiotics	247:256	arg1	class					228:232	an emerging class	216:232	an emerging class of potential prebiotics	216:256	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	9	theme	prebiotics	247:256	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	0	10	theme	flow	81:84	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	2	11	dep	reactor	430:436	arg1	using					444:448	using	444:448	using a low-cost crude enzyme mixture (viscozyme)	444:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	6	12	theme	average	1101:1107	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	0	13	theme	membrane	104:111	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	6	14	theme	%	1151:1151	arg1	productivity					1222:1233	a specific productivity	1211:1233	a specific productivity of 8.0 ± 1.0 g/g E/h	1211:1254	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	14	theme	%	1151:1151	arg1	productivity					1173:1184	a volumetric productivity	1160:1184	a volumetric productivity of 17.5 ± 2.1 g/L/h	1160:1204	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	14	theme	%	1151:1151	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	5	15	theme	obtained	792:799	arg1	mixtures					817:824	the obtained oligosaccharide mixtures	788:824	the obtained oligosaccharide mixtures	788:824	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	2	16	theme	main	263:266	arg1	aim					268:270	The main aim	259:270	The main aim of the present work	259:290	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	7	17	theme	mixtures	1355:1362	arg1	production					1325:1334	the continuous and stable production	1299:1334	the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1299:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	1	18	theme	attractive	151:160	arg1	pectin					138:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	18	theme	attractive	151:160	arg1	class					228:232	an emerging class	216:232	an emerging class of potential prebiotics	216:256	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	18	theme	attractive	151:160	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	0	19	theme	enzyme	97:102	arg1	reactor					113:119	a cross flow continuous enzyme membrane reactor	73:119	a cross flow continuous enzyme membrane reactor	73:119	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	0	20	theme	Continuous	0:9	arg1	production					11:20	Continuous production	0:20	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.	0:120	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	4	21	from	experiments	664:674	arg1	reactor					699:705	the enzyme membrane reactor	679:705	the enzyme membrane reactor	679:705	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	7	22	theme	oligosaccharide	1339:1353	arg1	mixtures					1355:1362	oligosaccharide mixtures	1339:1362	oligosaccharide mixtures	1339:1362	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	2	23	theme	flow	409:412	arg1	reactor					430:436	a cross flow enzyme membrane reactor	401:436	a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme)	401:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	4	24	theme	continuous	653:662	arg1	experiments					664:674	continuous experiments	653:674	continuous experiments in the enzyme membrane reactor	653:705	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	2	25	theme	cross	403:407	arg1	reactor					430:436	a cross flow enzyme membrane reactor	401:436	a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme)	401:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	6	26	theme	E/h	1252:1254	arg1	productivity					1222:1233	a specific productivity	1211:1233	a specific productivity of 8.0 ± 1.0 g/g E/h	1211:1254	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	26	theme	E/h	1252:1254	arg1	productivity					1173:1184	a volumetric productivity	1160:1184	a volumetric productivity of 17.5 ± 2.1 g/L/h	1160:1204	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	26	theme	E/h	1252:1254	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	2	27	theme	work	287:290	arg1	aim					268:270	The main aim	259:270	The main aim of the present work	259:290	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	7	28	theme	reactor	1407:1413	arg1	technology					1415:1424	enzyme membrane reactor technology	1391:1424	enzyme membrane reactor technology	1391:1424	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	2	29	theme	pectic	345:350	arg1	oligosaccharides					352:367	pectic oligosaccharides	345:367	pectic oligosaccharides	345:367	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	0	30	theme	pectic	25:30	arg1	oligosaccharides					32:47	pectic oligosaccharides	25:47	pectic oligosaccharides from sugar beet pulp	25:68	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	5	31	theme	oligosaccharide	801:815	arg1	mixtures					817:824	the obtained oligosaccharide mixtures	788:824	the obtained oligosaccharide mixtures	788:824	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	2	32	theme	present	279:285	arg1	work					287:290	the present work	275:290	the present work	275:290	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	7	33	theme	enzyme	1391:1396	arg1	technology					1415:1424	enzyme membrane reactor technology	1391:1424	enzyme membrane reactor technology	1391:1424	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	7	34	theme	continuous	1303:1312	arg1	production					1325:1334	the continuous and stable production	1299:1334	the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1299:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	3	35	theme	semi-continuous	532:546	arg1	setups					548:553	batch and semi-continuous setups	522:553	batch and semi-continuous setups	522:553	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	5	36	theme	condition-specific	929:946	arg1	arabinans					948:956	condition-specific arabinans	929:956	condition-specific arabinans	929:956	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	3	37	theme	batch	522:526	arg1	setups					548:553	batch and semi-continuous setups	522:553	batch and semi-continuous setups	522:553	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	5	38	theme	promising	875:883	arg1	conditions					885:894	the most promising conditions	866:894	the most promising conditions	866:894	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	7	39	theme	first	1288:1292	arg1	time					1294:1297	the first time	1284:1297	the first time	1284:1297	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	6	40	theme	8.0 ± 1.0 g/g	1238:1250	arg1	E/h					1252:1254	8.0 ± 1.0 g/g E/h	1238:1254	8.0 ± 1.0 g/g E/h	1238:1254	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	41	theme	optimized	1066:1074	arg1	conditions					1076:1085	the optimized conditions	1062:1085	the optimized conditions	1062:1085	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	1	42	theme	pectic	191:196	arg1	oligosaccharides					198:213	pectic oligosaccharides	191:213	pectic oligosaccharides	191:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	0	43	theme	oligosaccharides	32:47	arg1	production					11:20	Continuous production	0:20	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.	0:120	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	6	44	theme	continuous	1001:1010	arg1	production					1023:1032	A continuous and stable production	999:1032	A continuous and stable production	999:1032	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	5	45	theme	mixtures	817:824	arg1	composition					773:783	The composition	769:783	The composition of the obtained oligosaccharide mixtures	769:824	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	5	46	theme	molecular	846:854	arg1	level					856:860	the molecular level	842:860	the molecular level	842:860	The composition of the obtained oligosaccharide mixtures was assessed at the molecular level for the most promising conditions and was shown to be dominated by condition-specific arabinans, rhamnogalacturonans, and galacturonans.
30074061	1	47	theme	oligosaccharides	198:213	arg1	production					177:186	the production	173:186	the production of pectic oligosaccharides	173:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	0	48	theme	beet	60:63	arg1	pulp					65:68	sugar beet pulp	54:68	sugar beet pulp	54:68	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	0	49	from	pulp	65:68	arg1	production					11:20	Continuous production	0:20	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.	0:120	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	0	49	from	pulp	65:68	arg1	oligosaccharides					32:47	pectic oligosaccharides	25:47	pectic oligosaccharides from sugar beet pulp	25:68	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	6	50	theme	oligosaccharide	1116:1130	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	2	51	theme	new	313:315	arg1	process					317:323	a new process	311:323	a new process	311:323	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	0	52	theme	sugar	54:58	arg1	pulp					65:68	sugar beet pulp	54:68	sugar beet pulp	54:68	Continuous production of pectic oligosaccharides from sugar beet pulp in a cross flow continuous enzyme membrane reactor.
30074061	2	53	theme	enzyme	414:419	arg1	reactor					430:436	a cross flow enzyme membrane reactor	401:436	a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme)	401:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	6	54	theme	specific	1213:1220	arg1	productivity					1222:1233	a specific productivity	1211:1233	a specific productivity of 8.0 ± 1.0 g/g E/h	1211:1254	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	4	55	theme	membrane	690:697	arg1	reactor					699:705	the enzyme membrane reactor	679:705	the enzyme membrane reactor	679:705	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	6	56	theme	17.5 ± 2.1 g/L/h	1189:1204	arg1	productivity					1222:1233	a specific productivity	1211:1233	a specific productivity of 8.0 ± 1.0 g/g E/h	1211:1254	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	56	theme	17.5 ± 2.1 g/L/h	1189:1204	arg1	productivity					1173:1184	a volumetric productivity	1160:1184	a volumetric productivity of 17.5 ± 2.1 g/L/h	1160:1204	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	6	56	theme	17.5 ± 2.1 g/L/h	1189:1204	arg1	yield					1132:1136	an average pectic oligosaccharide yield	1098:1136	an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w)	1098:1157	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	7	57	theme	sugar	1369:1373	arg1	pulp					1380:1383	sugar beet pulp	1369:1383	sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1369:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	7	58	theme	membrane	1398:1405	arg1	technology					1415:1424	enzyme membrane reactor technology	1391:1424	enzyme membrane reactor technology	1391:1424	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	1	59	theme	Sugar	122:126	arg1	pectin					138:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	59	theme	Sugar	122:126	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	2	60	theme	enzyme	467:472	arg1	mixture					474:480	a low-cost crude enzyme mixture	450:480	a low-cost crude enzyme mixture (viscozyme)	450:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	2	60	theme	enzyme	467:472	arg1	viscozyme					483:491	viscozyme	483:491	viscozyme	483:491	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	7	61	theme	suitable	1437:1444	arg1	setup					1431:1435	a setup	1429:1435	a setup suitable for upscaling	1429:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	7	62	from	pulp	1380:1383	arg1	production					1325:1334	the continuous and stable production	1299:1334	the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1299:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	7	63	theme	beet	1375:1378	arg1	pulp					1380:1383	sugar beet pulp	1369:1383	sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1369:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	4	64	theme	substrate	727:735	arg1	loading					737:743	substrate loading	727:743	substrate loading	727:743	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	1	65	theme	beet	128:131	arg1	pectin					138:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	65	theme	beet	128:131	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	7	66	theme	stable	1318:1323	arg1	production					1325:1334	the continuous and stable production	1299:1334	the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling	1299:1458	This work demonstrated for the first time the continuous and stable production of oligosaccharide mixtures from sugar beet pulp using enzyme membrane reactor technology in a setup suitable for upscaling.
30074061	4	67	theme	residence	708:716	arg1	time					718:721	residence time	708:721	residence time	708:721	Then, in continuous experiments in the enzyme membrane reactor, residence time and substrate loading were further optimized.
30074061	2	68	theme	crude	461:465	arg1	mixture					474:480	a low-cost crude enzyme mixture	450:480	a low-cost crude enzyme mixture (viscozyme)	450:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	2	68	theme	crude	461:465	arg1	viscozyme					483:491	viscozyme	483:491	viscozyme	483:491	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	2	69	theme	continuous	374:383	arg1	way					385:387	a continuous way	372:387	a continuous way	372:387	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	3	70	theme	suitable	575:582	arg1	concentrations					591:604	suitable enzyme concentrations	575:604	suitable enzyme concentrations	575:604	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	1	71	theme	pulp	133:136	arg1	pectin					138:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin	122:143	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	71	theme	pulp	133:136	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	6	72	theme	volumetric	1162:1171	arg1	productivity					1173:1184	a volumetric productivity	1160:1184	a volumetric productivity of 17.5 ± 2.1 g/L/h	1160:1204	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
30074061	1	73	theme	emerging	219:226	arg1	class					228:232	an emerging class	216:232	an emerging class of potential prebiotics	216:256	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	1	73	theme	emerging	219:226	arg1	source					162:167	an attractive source	148:167	an attractive source for the production of pectic oligosaccharides	148:213	Sugar beet pulp pectin is an attractive source for the production of pectic oligosaccharides, an emerging class of potential prebiotics.
30074061	2	74	theme	membrane	421:428	arg1	reactor					430:436	a cross flow enzyme membrane reactor	401:436	a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme)	401:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	3	75	theme	enzyme	584:589	arg1	concentrations					591:604	suitable enzyme concentrations	575:604	suitable enzyme concentrations	575:604	Preliminary experiments in batch and semi-continuous setups allowed to identify suitable enzyme concentrations and assessing filtration suitability.
30074061	2	76	theme	low-cost	452:459	arg1	mixture					474:480	a low-cost crude enzyme mixture	450:480	a low-cost crude enzyme mixture (viscozyme)	450:492	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	2	76	theme	low-cost	452:459	arg1	viscozyme					483:491	viscozyme	483:491	viscozyme	483:491	The main aim of the present work was to investigate a new process allowing to produce pectic oligosaccharides in a continuous way by means of a cross flow enzyme membrane reactor while using a low-cost crude enzyme mixture (viscozyme).
30074061	6	77	theme	stable	1016:1021	arg1	production					1023:1032	A continuous and stable production	999:1032	A continuous and stable production	999:1032	A continuous and stable production was performed for 28.5 h at the optimized conditions, obtaining an average pectic oligosaccharide yield of 82.9 ± 9.9% (w/w), a volumetric productivity of 17.5 ± 2.1 g/L/h, and a specific productivity of 8.0 ± 1.0 g/g E/h.
31883888	6	0	theme	calvaria	945:952	arg1	cells					914:918	MC3T3-E1 cells	905:918	MC3T3-E1 cells	905:918	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	6	0	theme	calvaria	945:952	arg1	cells					932:936	primary cells	924:936	primary cells	924:936	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	0	1	theme	structural	73:82	arg1	characterization					84:99	structural characterization	73:99	structural characterization	73:99	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	1	2	theme	important	161:169	arg1	role					171:174	an important role	158:174	an important role	158:174	Glycosaminoglycans (GAGs) play an important role in various biological activities.
31883888	2	3	attach	present	228:234	arg2	lot					212:214	A lot	210:214	A lot of them	210:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	3	attach	present	228:234	arg2	present					228:234	present	228:234	present	228:234	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	3	attach	present	228:234	arg2	them					219:222	them	219:222	them	219:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	3	attach	present	228:234	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	3	4	dep	processing	422:431	arg1	discard					433:439	discard	433:439	discard	433:439	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	2	5	theme	fish	283:286	arg1	industries					299:308	fish processing industries	283:308	fish processing industries	283:308	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	5	theme	fish	283:286	arg1	abattoirs					269:277	abattoirs	269:277	abattoirs	269:277	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	5	theme	fish	283:286	arg1	source					340:345	a valuable source	329:345	a valuable source of GAGs	329:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	5	6	contain	have	751:754	arg1	CS/DS					726:730	CS/DS	726:730	CS/DS from both the fish	726:749	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	5	6	contain	have	751:754	arg2	composition					780:790	a distinct disaccharide composition	756:790	a distinct disaccharide composition indicating differences in their structure	756:832	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	4	7	theme	sulfate/dermatan	666:681	arg1	CS/DS					692:696	CS/DS	692:696	CS/DS	692:696	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	7	theme	sulfate/dermatan	666:681	arg1	sulfate					683:689	chondroitin sulfate/dermatan sulfate	654:689	chondroitin sulfate/dermatan sulfate (CS/DS)	654:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	3	8	theme	rohita	487:492	arg1	processing					467:476	the processing	463:476	the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus)	463:547	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	0	9	theme	osteogenic	106:115	arg1	activity					117:124	osteogenic activity	106:124	osteogenic activity	106:124	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	0	10	from	processing	41:50	arg1	Glycosaminoglycans					0:17	Glycosaminoglycans	0:17	Glycosaminoglycans from fresh water fish processing	0:50	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	2	11	from	present	228:234	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	5	12	from	fish	746:749	arg1	CS/DS					726:730	CS/DS	726:730	CS/DS from both the fish	726:749	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	1	13	theme	various	179:185	arg1	activities					198:207	various biological activities	179:207	various biological activities	179:207	Glycosaminoglycans (GAGs) play an important role in various biological activities.
31883888	6	14	theme	primary	924:930	arg1	cells					932:936	primary cells	924:936	primary cells	924:936	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	3	15	theme	brachypomus	520:530	arg1	processing					467:476	the processing	463:476	the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus)	463:547	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	1	16	theme	biological	187:196	arg1	activities					198:207	various biological activities	179:207	various biological activities	179:207	Glycosaminoglycans (GAGs) play an important role in various biological activities.
31883888	0	17	theme	water	30:34	arg1	processing					41:50	fresh water fish processing	24:50	fresh water fish processing	24:50	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	6	18	theme	Biological	835:844	arg1	activity					846:853	Biological activity	835:853	Biological activity	835:853	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	4	19	theme	heparan	704:710	arg1	HS					721:722	HS	721:722	HS	721:722	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	19	theme	heparan	704:710	arg1	sulfate					712:718	heparan sulfate	704:718	heparan sulfate (HS)	704:723	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	5	20	theme	distinct	758:765	arg1	composition					780:790	a distinct disaccharide composition	756:790	a distinct disaccharide composition indicating differences in their structure	756:832	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	0	21	theme	fresh	24:28	arg1	processing					41:50	fresh water fish processing	24:50	fresh water fish processing	24:50	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	2	22	theme	processing	288:297	arg1	industries					299:308	fish processing industries	283:308	fish processing industries	283:308	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	22	theme	processing	288:297	arg1	abattoirs					269:277	abattoirs	269:277	abattoirs	269:277	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	22	theme	processing	288:297	arg1	source					340:345	a valuable source	329:345	a valuable source of GAGs	329:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	23	from	discards	255:262	arg1	present					228:234	present	228:234	present	228:234	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	23	from	discards	255:262	arg1	them					219:222	them	219:222	them	219:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	23	from	discards	255:262	arg1	lot					212:214	A lot	210:214	A lot of them	210:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	5	24	theme	disaccharide	767:778	arg1	composition					780:790	a distinct disaccharide composition	756:790	a distinct disaccharide composition indicating differences in their structure	756:832	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	6	25	theme	MC3T3-E1	905:912	arg1	cells					914:918	MC3T3-E1 cells	905:918	MC3T3-E1 cells	905:918	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	3	26	theme	osteogenic	594:603	arg1	activity					605:612	osteogenic activity	594:612	osteogenic activity	594:612	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	4	27	theme	Isolated	615:622	arg1	GAGs					624:627	Isolated GAGs	615:627	Isolated GAGs	615:627	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	3	28	theme	fish	417:420	arg1	processing					422:431	fish processing discard	417:439	fish processing discard	417:439	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	0	29	theme	fish	36:39	arg1	processing					41:50	fresh water fish processing	24:50	fresh water fish processing	24:50	Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity.
31883888	2	30	located	present	228:234	arg2	lot					212:214	A lot	210:214	A lot of them	210:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	30	located	present	228:234	arg2	present					228:234	present	228:234	present	228:234	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	30	located	present	228:234	arg2	them					219:222	them	219:222	them	219:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	30	located	present	228:234	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	6	31	from	activity	846:853	arg1	terms					859:863	terms	859:863	terms of promoting osteogenesis	859:889	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	2	32	theme	them	219:222	arg1	present					228:234	present	228:234	present	228:234	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	32	theme	them	219:222	arg1	them					219:222	them	219:222	them	219:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	32	theme	them	219:222	arg1	lot					212:214	A lot	210:214	A lot of them	210:222	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	3	33	from	processing	422:431	arg1	GAGs					407:410	GAGs	407:410	GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus)	407:547	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	3	33	from	processing	422:431	arg1	head					442:445	head	442:445	head	442:445	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	2	34	theme	valuable	331:338	arg1	industries					299:308	fish processing industries	283:308	fish processing industries	283:308	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	34	theme	valuable	331:338	arg1	abattoirs					269:277	abattoirs	269:277	abattoirs	269:277	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	34	theme	valuable	331:338	arg1	source					340:345	a valuable source	329:345	a valuable source of GAGs	329:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	6	35	theme	phosphatase	1014:1024	arg1	staining					1026:1033	alkaline phosphatase staining	1005:1033	alkaline phosphatase staining	1005:1033	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	4	36	theme	chondroitin	654:664	arg1	CS/DS					692:696	CS/DS	692:696	CS/DS	692:696	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	36	theme	chondroitin	654:664	arg1	sulfate					683:689	chondroitin sulfate/dermatan sulfate	654:689	chondroitin sulfate/dermatan sulfate (CS/DS)	654:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	2	37	from	abattoirs	269:277	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	6	38	theme	osteogenesis	878:889	arg1	terms					859:863	terms	859:863	terms of promoting osteogenesis	859:889	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	2	39	theme	processing	244:253	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	4	40	theme	sulfate	683:689	arg1	amounts					643:649	higher amounts	636:649	higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS)	636:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	40	theme	sulfate	683:689	arg1	CS/DS					692:696	CS/DS	692:696	CS/DS	692:696	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	40	theme	sulfate	683:689	arg1	sulfate					683:689	chondroitin sulfate/dermatan sulfate	654:689	chondroitin sulfate/dermatan sulfate (CS/DS)	654:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	6	41	theme	early	966:970	arg1	mineralization					972:985	early mineralization	966:985	early mineralization	966:985	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	6	42	theme	late	1053:1056	arg1	mineralization					1058:1071	late mineralization	1053:1071	late mineralization	1053:1071	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	2	43	theme	fish	239:242	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	6	44	theme	alkaline	1005:1012	arg1	phosphatase					1014:1024	alkaline phosphatase	1005:1024	alkaline phosphatase staining	1005:1033	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	4	45	theme	higher	636:641	arg1	amounts					643:649	higher amounts	636:649	higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS)	636:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	45	theme	higher	636:641	arg1	CS/DS					692:696	CS/DS	692:696	CS/DS	692:696	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	4	45	theme	higher	636:641	arg1	sulfate					683:689	chondroitin sulfate/dermatan sulfate	654:689	chondroitin sulfate/dermatan sulfate (CS/DS)	654:697	Isolated GAGs showed higher amounts of chondroitin sulfate/dermatan sulfate (CS/DS) than heparan sulfate (HS).
31883888	3	46	dep	brachypomus	520:530	arg1	brachypomus					536:546	P. brachypomus	533:546	P. brachypomus	533:546	We have, in this paper, isolated and characterized GAGs from fish processing discard (head) generated from the processing of Labeo rohita (L. rohita) and Piaractus brachypomus (P. brachypomus) and have determined their ability to promote osteogenic activity.
31883888	6	47	theme	promoting	868:876	arg1	osteogenesis					878:889	promoting osteogenesis	868:889	promoting osteogenesis	868:889	Biological activity, in terms of promoting osteogenesis, evaluated in MC3T3-E1 cells and primary cells of the calvaria showed that early mineralization, characterized by alkaline phosphatase staining and activity, and late mineralization, was supported by both the GAGs.
31883888	5	48	from	differences	803:813	arg1	structure					824:832	their structure	818:832	their structure	818:832	CS/DS from both the fish have a distinct disaccharide composition indicating differences in their structure.
31883888	2	49	theme	GAGs	350:353	arg1	industries					299:308	fish processing industries	283:308	fish processing industries	283:308	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	49	theme	GAGs	350:353	arg1	abattoirs					269:277	abattoirs	269:277	abattoirs	269:277	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	49	theme	GAGs	350:353	arg1	source					340:345	a valuable source	329:345	a valuable source of GAGs	329:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
31883888	2	50	from	industries	299:308	arg1	discards					255:262	fish processing discards	239:262	fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs	239:353	A lot of them are present in fish processing discards from abattoirs and fish processing industries which can serve as a valuable source of GAGs.
29926199	0	0	theme	graphitic	99:107	arg1	dots					141:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	2	1	with	600 nm	389:394	arg1	yield					411:415	a quantum yield	401:415	a quantum yield of 21%	401:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	7	2	theme	silver	958:963	arg1	nanoparticles					965:977	silver nanoparticles	958:977	silver nanoparticles	958:977	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	4	3	theme	heparin	567:573	arg1	addition					555:562	addition	555:562	addition of heparin	555:573	Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs.
29926199	8	4	theme	heparin	1125:1131	arg1	addition					1113:1120	addition	1113:1120	addition of heparin	1113:1131	Its red fluorescence is weak in presence of polyethyleneimine but restored on addition of heparin.
29926199	6	5	theme	detection	863:871	arg1	limit					854:858	a 8.2 nM limit	845:858	a 8.2 nM limit of detection	845:871	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	6	6	theme	concentration	819:831	arg1	range					833:837	the 0.025 to 2.5 μM heparin concentration range	791:837	the 0.025 to 2.5 μM heparin concentration range	791:837	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	1	7	theme	water	256:260	arg1	synthesis					268:276	water phase synthesis	256:276	water phase synthesis	256:276	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	0	8	theme	nitride	116:122	arg1	dots					141:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	9	9	theme	a new	1159:1163	arg1	method					1165:1170	a new method	1159:1170	a new method for heparin detection	1159:1192	This forms the basis for a new method for heparin detection.
29926199	2	10	theme	metal-enhanced	288:301	arg1	fluorescence					303:314	metal-enhanced fluorescence	288:314	metal-enhanced fluorescence	288:314	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	1	11	theme	phase	262:266	arg1	synthesis					268:276	water phase synthesis	256:276	water phase synthesis	256:276	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	0	12	theme	carbon	109:114	arg1	dots					141:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	5	13	used	used	697:700	arg2	effect					686:691	The effect	682:691	The effect	682:691	The effect was used to design a fluorometric assay for heparin.
29926199	0	14	theme	g-C3N4	125:130	arg1	dots					141:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	6	15	theme	8.2 nM	847:852	arg1	limit					854:858	a 8.2 nM limit	845:858	a 8.2 nM limit of detection	845:871	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	2	16	theme	quantum	403:409	arg1	yield					411:415	a quantum yield	401:415	a quantum yield of 21%	401:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	6	17	theme	at	759:760	arg1	600 nm					762:767	The emission at 600 nm	746:767	The emission at 600 nm	746:767	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	6	18	from	600 nm	762:767	arg1	emission					750:757	The emission at 600 nm	746:767	The emission at 600 nm	746:767	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	6	18	from	600 nm	762:767	arg1	at					759:760	The emission at 600 nm	746:767	The emission at 600 nm	746:767	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	6	19	theme	emission	750:757	arg1	600 nm					762:767	The emission at 600 nm	746:767	The emission at 600 nm	746:767	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	0	20	theme	Ultrasensitive	0:13	arg1	detection					15:23	Ultrasensitive detection	0:23	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.	0:145	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	7	21	theme	abstract	884:891	arg1	illustration					903:914	Graphical abstract Schematic illustration	874:914	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).	874:1033	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	4	22	theme	higher	637:642	arg1	affinity					644:651	a higher affinity	635:651	a higher affinity for heparin than Ag-g-CNQDs	635:679	Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs.
29926199	0	23	theme	heparin	28:34	arg1	detection					15:23	Ultrasensitive detection	0:23	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.	0:145	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	0	24	theme	quantum	133:139	arg1	dots					141:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	graphitic carbon nitride (g-C3N4) quantum dots	99:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	7	25	theme	Schematic	893:901	arg1	illustration					903:914	Graphical abstract Schematic illustration	874:914	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).	874:1033	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	2	26	theme	%	422:422	arg1	yield					411:415	a quantum yield	401:415	a quantum yield of 21%	401:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	1	27	theme	carbon	200:205	arg1	dots					223:226	graphitic carbon nitride quantum dots	190:226	graphitic carbon nitride quantum dots	190:226	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	1	28	theme	nitride	207:213	arg1	dots					223:226	graphitic carbon nitride quantum dots	190:226	graphitic carbon nitride quantum dots	190:226	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	0	29	theme	dots	141:144	arg1	fluorescence					83:94	the silver nanoparticle-enhanced fluorescence	50:94	the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots	50:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	2	30	with	emission	365:372	arg1	peak					381:384	a peak	379:384	a peak at 600 nm with a quantum yield of 21%	379:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	4	31	theme	system	600:605	arg1	fluorescence					580:591	the fluorescence	576:591	the fluorescence of the system	576:605	Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs.
29926199	7	32	theme	nitride	1000:1006	arg1	Ag-g-CNQDs					1022:1031	Ag-g-CNQDs	1022:1031	Ag-g-CNQDs	1022:1031	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	7	32	theme	nitride	1000:1006	arg1	dots					1016:1019	graphitic carbon nitride quantum dots	983:1019	graphitic carbon nitride quantum dots (Ag-g-CNQDs)	983:1032	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	1	33	theme	quantum	215:221	arg1	dots					223:226	graphitic carbon nitride quantum dots	190:226	graphitic carbon nitride quantum dots	190:226	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	0	34	theme	silver	54:59	arg1	fluorescence					83:94	the silver nanoparticle-enhanced fluorescence	50:94	the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots	50:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	6	35	theme	heparin	811:817	arg1	range					833:837	the 0.025 to 2.5 μM heparin concentration range	791:837	the 0.025 to 2.5 μM heparin concentration range	791:837	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	2	36	from	600 nm	389:394	arg1	peak					381:384	a peak	379:384	a peak at 600 nm with a quantum yield of 21%	379:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	8	37	theme	polyethyleneimine	1079:1095	arg1	presence					1067:1074	presence	1067:1074	presence of polyethyleneimine	1067:1095	Its red fluorescence is weak in presence of polyethyleneimine but restored on addition of heparin.
29926199	7	38	theme	graphitic	983:991	arg1	Ag-g-CNQDs					1022:1031	Ag-g-CNQDs	1022:1031	Ag-g-CNQDs	1022:1031	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	7	38	theme	graphitic	983:991	arg1	dots					1016:1019	graphitic carbon nitride quantum dots	983:1019	graphitic carbon nitride quantum dots (Ag-g-CNQDs)	983:1032	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	2	39	theme	red	361:363	arg1	emission					365:372	excitation-dependent red emission	340:372	excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%	340:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	6	40	dep	range	833:837	arg1	to					801:802	to	801:802	to	801:802	The emission at 600 nm increases linearly in the 0.025 to 2.5 μM heparin concentration range, with a 8.2 nM limit of detection.
29926199	9	41	theme	heparin	1176:1182	arg1	detection					1184:1192	heparin detection	1176:1192	heparin detection	1176:1192	This forms the basis for a new method for heparin detection.
29926199	7	42	theme	carbon	993:998	arg1	Ag-g-CNQDs					1022:1031	Ag-g-CNQDs	1022:1031	Ag-g-CNQDs	1022:1031	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	7	42	theme	carbon	993:998	arg1	dots					1016:1019	graphitic carbon nitride quantum dots	983:1019	graphitic carbon nitride quantum dots (Ag-g-CNQDs)	983:1032	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	2	43	theme	excitation-dependent	340:359	arg1	emission					365:372	excitation-dependent red emission	340:372	excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%	340:422	Aided by metal-enhanced fluorescence, the composite exhibits excitation-dependent red emission with a peak at 600 nm with a quantum yield of 21%.
29926199	7	44	theme	Graphical	874:882	arg1	illustration					903:914	Graphical abstract Schematic illustration	874:914	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).	874:1033	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	4	45	contain	has	631:633	arg2	affinity					644:651	a higher affinity	635:651	a higher affinity for heparin than Ag-g-CNQDs	635:679	Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs.
29926199	4	45	contain	has	631:633	arg1	PEI					627:629	PEI	627:629	PEI	627:629	Upon addition of heparin, the fluorescence of the system is enhanced because PEI has a higher affinity for heparin than Ag-g-CNQDs.
29926199	7	46	theme	quantum	1008:1014	arg1	Ag-g-CNQDs					1022:1031	Ag-g-CNQDs	1022:1031	Ag-g-CNQDs	1022:1031	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	7	46	theme	quantum	1008:1014	arg1	dots					1016:1019	graphitic carbon nitride quantum dots	983:1019	graphitic carbon nitride quantum dots (Ag-g-CNQDs)	983:1032	Graphical abstract Schematic illustration for fabricating a composite consisting of silver nanoparticles and graphitic carbon nitride quantum dots (Ag-g-CNQDs).
29926199	8	47	from	presence	1067:1074	arg1	weak					1059:1062	weak	1059:1062	weak	1059:1062	Its red fluorescence is weak in presence of polyethyleneimine but restored on addition of heparin.
29926199	5	48	theme	fluorometric	714:725	arg1	assay					727:731	a fluorometric assay	712:731	a fluorometric assay for heparin	712:743	The effect was used to design a fluorometric assay for heparin.
29926199	1	49	theme	graphitic	190:198	arg1	dots					223:226	graphitic carbon nitride quantum dots	190:226	graphitic carbon nitride quantum dots	190:226	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	3	50	theme	Ag-g-CNQD/PEI	492:504	arg1	complexe					506:513	the Ag-g-CNQD/PEI complexe	488:513	the Ag-g-CNQD/PEI complexe	488:513	If the composite is coated with polyethylenimine (PEI) to form the Ag-g-CNQD/PEI complexe, fluorescence is strongly reduced.
29926199	1	51	theme	silver	232:237	arg1	nanoparticles					239:251	silver nanoparticles	232:251	silver nanoparticles	232:251	A composite (Ag-g-CNQDs) was prepared from graphitic carbon nitride quantum dots and silver nanoparticles by water phase synthesis.
29926199	0	52	theme	nanoparticle-enhanced	61:81	arg1	fluorescence					83:94	the silver nanoparticle-enhanced fluorescence	50:94	the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots	50:144	Ultrasensitive detection of heparin by exploiting the silver nanoparticle-enhanced fluorescence of graphitic carbon nitride (g-C3N4) quantum dots.
29926199	8	53	from	weak	1059:1062	arg1	presence					1067:1074	presence	1067:1074	presence of polyethyleneimine	1067:1095	Its red fluorescence is weak in presence of polyethyleneimine but restored on addition of heparin.
30562892	0	0	theme	acid/Multiwalled	96:111	arg1	Nanotubes/Extract					120:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract	68:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites	68:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	5	1	theme	energy	848:853	arg1	spectroscopy					866:877	energy dispersive spectroscopy	848:877	energy dispersive spectroscopy	848:877	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	5	2	dep	structure	742:750	arg1	The					738:740	The	738:740	The	738:740	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	8	3	theme	Mimosa	1256:1261	arg1	tenuiflora					1263:1272	Mimosa tenuiflora	1256:1272	Mimosa tenuiflora	1256:1272	It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
30562892	9	4	theme	composites	1429:1438	arg1	potential					1450:1458	their potential	1444:1458	their potential as candidates for tissue engineering applications	1444:1508	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	9	4	theme	composites	1429:1438	arg1	efficiency					1387:1396	the efficiency	1383:1396	the efficiency of bioactive and biocompatible composites	1383:1438	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	4	5	theme	simulated	689:697	arg1	fluid					704:708	simulated body fluid	689:708	simulated body fluid for 7, 14, 21, and 28 days	689:735	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	0	6	theme	Polycaprolactone/Hyaluronic	68:94	arg1	Nanotubes/Extract					120:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract	68:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites	68:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	6	7	theme	alkaline	1004:1011	arg1	phosphatase					1013:1023	alkaline phosphatase	1004:1023	alkaline phosphatase activity of the osteoblasts	1004:1051	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	1	8	theme	biomaterial	203:213	arg1	scaffolds					215:223	biomaterial scaffolds	203:223	biomaterial scaffolds	203:223	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	0	9	theme	Nanotubes/Extract	120:136	arg1	biocompatibility					48:63	in vitro biocompatibility	39:63	in vitro biocompatibility	39:63	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	0	9	theme	Nanotubes/Extract	120:136	arg1	bioactivity					23:33	in vitro bioactivity	14:33	in vitro bioactivity	14:33	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	2	10	theme	Carbon	351:356	arg1	Nanotubes/Extract					358:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract	303:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites	303:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	2	11	theme	composites	397:406	arg1	Nanotubes/Extract					358:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract	303:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites	303:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	9	12	theme	biocompatible	1415:1427	arg1	composites					1429:1438	bioactive and biocompatible composites	1401:1438	bioactive and biocompatible composites	1401:1438	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	0	13	theme	Carbon	113:118	arg1	Nanotubes/Extract					120:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract	68:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites	68:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	5	14	theme	composites	775:784	arg1	composition					756:766	composition	756:766	composition	756:766	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	5	14	theme	composites	775:784	arg1	structure					742:750	structure	742:750	structure	742:750	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	2	15	theme	tenuiflora	386:395	arg1	composites					397:406	Mimosa tenuiflora composites	379:406	Mimosa tenuiflora composites	379:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	1	16	dep	BACKGROUND	173:182	arg1	development					188:198	The development	184:198	The development of biomaterial scaffolds	184:223	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	1	16	dep	BACKGROUND	173:182	arg1	necessary					281:289	necessary	281:289	necessary	281:289	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	4	17	theme	body	699:702	arg1	fluid					704:708	simulated body fluid	689:708	simulated body fluid for 7, 14, 21, and 28 days	689:735	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	0	18	theme	Mimosa	143:148	arg1	composites					161:170	Mimosa tenuiflora composites	143:170	Mimosa tenuiflora composites	143:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	7	19	with	morphology	1176:1185	arg1	bioactivity					1201:1211	enhanced bioactivity	1192:1211	enhanced bioactivity	1192:1211	RESULTS The results show a porous and interconnected morphology with enhanced bioactivity.
30562892	5	20	dep	Fourier	883:889	arg1	transform					891:899	transform	891:899	transform infrared spectroscopy	891:921	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	2	21	theme	Mimosa	379:384	arg1	composites					397:406	Mimosa tenuiflora composites	379:406	Mimosa tenuiflora composites	379:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	8	22	theme	increased	1301:1309	arg1	viability					1311:1319	increased viability	1301:1319	increased viability of osteoblasts	1301:1334	It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
30562892	1	23	theme	scaffolds	215:223	arg1	implementation					229:242	implementation	229:242	implementation of tissue engineering techniques	229:275	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	1	23	theme	scaffolds	215:223	arg1	development					188:198	The development	184:198	The development of biomaterial scaffolds	184:223	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	1	23	theme	scaffolds	215:223	arg1	necessary					281:289	necessary	281:289	necessary	281:289	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	5	24	dep	transform	891:899	arg1	infrared					901:908	infrared	901:908	transform infrared spectroscopy	891:921	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	7	25	theme	interconnected	1161:1174	arg1	morphology					1176:1185	a porous and interconnected morphology	1148:1185	a porous and interconnected morphology with enhanced bioactivity	1148:1211	RESULTS The results show a porous and interconnected morphology with enhanced bioactivity.
30562892	5	26	theme	electron	815:822	arg1	microscopy					824:833	scanning electron microscopy	806:833	scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy	806:921	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	0	27	from	bioactivity	23:33	arg1	composites					161:170	Mimosa tenuiflora composites	143:170	Mimosa tenuiflora composites	143:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	6	28	theme	metabolic	1074:1082	arg1	activity					1084:1091	the metabolic activity	1070:1091	the metabolic activity of the cells	1070:1104	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	3	29	theme	in	536:537	arg1	bioactivity					545:555	in vitro bioactivity	536:555	in vitro bioactivity	536:555	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	3	30	theme	research	507:514	arg1	objective					489:497	The objective	485:497	The objective of this research	485:514	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	4	31	dep	METHODS	606:612	arg1	bioactivity					627:637	The in vitro bioactivity	614:637	METHODS The in vitro bioactivity of the composites	606:655	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	7	32	theme	porous	1150:1155	arg1	morphology					1176:1185	a porous and interconnected morphology	1148:1185	a porous and interconnected morphology with enhanced bioactivity	1148:1211	RESULTS The results show a porous and interconnected morphology with enhanced bioactivity.
30562892	7	33	theme	enhanced	1192:1199	arg1	bioactivity					1201:1211	enhanced bioactivity	1192:1211	enhanced bioactivity	1192:1211	RESULTS The results show a porous and interconnected morphology with enhanced bioactivity.
30562892	6	34	theme	cells	1100:1104	arg1	activity					1084:1091	the metabolic activity	1070:1091	the metabolic activity of the cells	1070:1104	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	8	35	from	incorporation	1239:1251	arg1	composites					1281:1290	the composites	1277:1290	the composites	1277:1290	It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
30562892	7	36	dep	RESULTS	1123:1129	arg1	results					1135:1141	The results	1131:1141	RESULTS The results	1123:1141	RESULTS The results show a porous and interconnected morphology with enhanced bioactivity.
30562892	1	37	theme	tissue	247:252	arg1	techniques					266:275	tissue engineering techniques	247:275	tissue engineering techniques	247:275	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	0	38	theme	in	14:15	arg1	bioactivity					23:33	in vitro bioactivity	14:33	in vitro bioactivity	14:33	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	0	39	theme	tenuiflora	150:159	arg1	composites					161:170	Mimosa tenuiflora composites	143:170	Mimosa tenuiflora composites	143:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	6	40	theme	composites	967:976	arg1	biocompatibility					943:958	the in vitro biocompatibility	930:958	the in vitro biocompatibility of the composites	930:976	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	6	41	theme	phosphatase	1013:1023	arg1	activity					1025:1032	alkaline phosphatase activity	1004:1032	alkaline phosphatase activity of the osteoblasts	1004:1051	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	1	42	theme	engineering	254:264	arg1	techniques					266:275	tissue engineering techniques	247:275	tissue engineering techniques	247:275	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	8	43	theme	osteoblasts	1324:1334	arg1	viability					1311:1319	increased viability	1301:1319	increased viability of osteoblasts	1301:1334	It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
30562892	6	44	dep	in	934:935	arg1	vitro					937:941	vitro	937:941	vitro	937:941	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	3	45	theme	in	561:562	arg1	biocompatibility					570:585	in vitro biocompatibility	561:585	in vitro biocompatibility	561:585	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	3	46	dep	in	536:537	arg1	vitro					539:543	vitro	539:543	vitro	539:543	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	1	47	theme	techniques	266:275	arg1	implementation					229:242	implementation	229:242	implementation of tissue engineering techniques	229:275	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	1	47	theme	techniques	266:275	arg1	development					188:198	The development	184:198	The development of biomaterial scaffolds	184:223	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	1	47	theme	techniques	266:275	arg1	necessary					281:289	necessary	281:289	necessary	281:289	BACKGROUND The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary.
30562892	0	48	theme	bioactivity	23:33	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.	0:171	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	9	49	theme	engineering	1485:1495	arg1	applications					1497:1508	tissue engineering applications	1478:1508	tissue engineering applications	1478:1508	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	5	50	theme	dispersive	855:864	arg1	spectroscopy					866:877	energy dispersive spectroscopy	848:877	energy dispersive spectroscopy	848:877	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30562892	0	51	dep	in	39:40	arg1	vitro					42:46	vitro	42:46	vitro	42:46	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	6	52	theme	osteoblasts	1041:1051	arg1	activity					1025:1032	alkaline phosphatase activity	1004:1032	alkaline phosphatase activity of the osteoblasts	1004:1051	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	0	53	from	biocompatibility	48:63	arg1	composites					161:170	Mimosa tenuiflora composites	143:170	Mimosa tenuiflora composites	143:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	3	54	dep	bioactivity	545:555	arg1	the					532:534	the	532:534	the	532:534	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	2	55	theme	separation	456:465	arg1	method					467:472	a thermally-induced phase separation method	430:472	a thermally-induced phase separation method	430:472	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	0	56	theme	in	39:40	arg1	biocompatibility					48:63	in vitro biocompatibility	39:63	in vitro biocompatibility	39:63	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	4	57	dep	in	618:619	arg1	vitro					621:625	vitro	621:625	vitro	621:625	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	9	58	theme	bioactive	1401:1409	arg1	composites					1429:1438	bioactive and biocompatible composites	1401:1438	bioactive and biocompatible composites	1401:1438	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	2	59	theme	phase	450:454	arg1	method					467:472	a thermally-induced phase separation method	430:472	a thermally-induced phase separation method	430:472	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	6	60	theme	MTT	1112:1114	arg1	assay					1116:1120	MTT assay	1112:1120	MTT assay	1112:1120	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	4	61	theme	composites	646:655	arg1	bioactivity					627:637	The in vitro bioactivity	614:637	METHODS The in vitro bioactivity of the composites	606:655	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	3	62	dep	OBJECTIVE	475:483	arg1	was					516:518	was	516:518	was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites	516:603	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	6	63	theme	in	934:935	arg1	biocompatibility					943:958	the in vitro biocompatibility	930:958	the in vitro biocompatibility of the composites	930:976	Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay.
30562892	3	64	dep	in	561:562	arg1	vitro					564:568	vitro	564:568	vitro	564:568	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	2	65	theme	thermally-induced	432:448	arg1	method					467:472	a thermally-induced phase separation method	430:472	a thermally-induced phase separation method	430:472	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	8	66	theme	tenuiflora	1263:1272	arg1	incorporation					1239:1251	the incorporation	1235:1251	the incorporation of Mimosa tenuiflora in the composites	1235:1290	It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds.
30562892	0	67	dep	in	14:15	arg1	vitro					17:21	vitro	17:21	vitro	17:21	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	3	68	theme	composites	594:603	arg1	bioactivity					545:555	in vitro bioactivity	536:555	in vitro bioactivity	536:555	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	3	68	theme	composites	594:603	arg1	biocompatibility					570:585	in vitro biocompatibility	561:585	in vitro biocompatibility	561:585	OBJECTIVE The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites.
30562892	2	69	theme	Hyaluronate/Multiwalled	327:349	arg1	Nanotubes/Extract					358:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract	303:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites	303:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	9	70	theme	tissue	1478:1483	arg1	applications					1497:1508	tissue engineering applications	1478:1508	tissue engineering applications	1478:1508	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	0	71	from	composites	161:170	arg1	Nanotubes/Extract					120:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract	68:136	Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites	68:170	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	0	71	from	composites	161:170	arg1	biocompatibility					48:63	in vitro biocompatibility	39:63	in vitro biocompatibility	39:63	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	0	71	from	composites	161:170	arg1	bioactivity					23:33	in vitro bioactivity	14:33	in vitro bioactivity	14:33	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	0	72	theme	biocompatibility	48:63	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.	0:171	Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites.
30562892	4	73	theme	in	618:619	arg1	bioactivity					627:637	The in vitro bioactivity	614:637	METHODS The in vitro bioactivity of the composites	606:655	METHODS The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days.
30562892	2	74	theme	Polycaprolactone/Sodium	303:325	arg1	Nanotubes/Extract					358:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract	303:374	Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites	303:406	Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method.
30562892	9	75	dep	CONCLUSIONS	1354:1364	arg1	show					1378:1381	show	1378:1381	show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications	1378:1508	CONCLUSIONS The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
30562892	5	76	theme	scanning	806:813	arg1	microscopy					824:833	scanning electron microscopy	806:833	scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy	806:921	The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy.
30946908	0	0	theme	transformer	112:122	arg1	oil					124:126	transformer oil	112:126	transformer oil	112:126	Preparation and characterisation of carboxymethyl-chitosan/sodium phytate composite membranes for adsorption in transformer oil.
30946908	4	1	from	effects	587:593	arg1	adsorption					671:680	adsorption	671:680	adsorption of copper, iron, and aluminium impurities	671:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	4	2	theme	temperature	656:666	arg1	content					605:611	the SP content	598:611	the SP content of the membrane, contact time, and contact temperature	598:666	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	6	3	theme	camera	868:873	arg1	observations					875:886	The SEM and video camera observations	850:886	The SEM and video camera observations	850:886	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	6	4	theme	video	862:866	arg1	camera					868:873	video camera	862:873	video camera	862:873	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	4	5	theme	contact	648:654	arg1	temperature					656:666	contact temperature	648:666	contact temperature	648:666	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	7	6	theme	membrane	1070:1077	arg1	capacity					1052:1059	the adsorption capacity	1037:1059	the adsorption capacity of a CMC membrane	1037:1077	The experimental results showed that the SP content significantly affected the adsorption capacity of a CMC membrane.
30946908	3	7	dep	spectra	473:479	arg1	observations					569:580	observations	569:580	observations	569:580	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	8	8	theme	copper	1128:1133	arg1	percentages					1103:1113	The maximum adsorption percentages	1080:1113	The maximum adsorption percentages of elemental copper, iron, and aluminium	1080:1154	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	8	theme	copper	1128:1133	arg1	%					1166:1166	88.12%	1161:1166	88.12%	1161:1166	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	6	9	theme	SEM	854:856	arg1	observations					875:886	The SEM and video camera observations	850:886	The SEM and video camera observations	850:886	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	1	10	from	oil	177:179	arg1	Adsorption					129:138	Adsorption	129:138	Adsorption of metal impurities from transformer oil	129:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	1	10	from	oil	177:179	arg1	impurities					149:158	metal impurities	143:158	metal impurities from transformer oil	143:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	4	11	theme	aluminium	703:711	arg1	impurities					713:722	aluminium impurities	703:722	aluminium impurities	703:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	3	12	theme	scanning	482:489	arg1	SEM					512:514	SEM	512:514	SEM	512:514	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	3	12	theme	scanning	482:489	arg1	microscopy					500:509	scanning electron microscopy	482:509	scanning electron microscopy (SEM)	482:515	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	5	13	theme	membranes	791:799	arg1	spectra					747:753	The FTIR spectra	738:753	The FTIR spectra	738:753	The FTIR spectra and thermogravimetric curves of the membranes indicated good compatibility between CMC and SP.
30946908	5	13	theme	membranes	791:799	arg1	curves					777:782	thermogravimetric curves	759:782	thermogravimetric curves	759:782	The FTIR spectra and thermogravimetric curves of the membranes indicated good compatibility between CMC and SP.
30946908	4	14	theme	SP	602:603	arg1	content					605:611	the SP content	598:611	the SP content of the membrane, contact time, and contact temperature	598:666	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	4	15	theme	time	638:641	arg1	content					605:611	the SP content	598:611	the SP content of the membrane, contact time, and contact temperature	598:666	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	3	16	theme	membranes	431:439	arg1	structure					414:422	The chemical structure	401:422	The chemical structure of the membranes	401:439	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	8	17	theme	iron	1136:1139	arg1	percentages					1103:1113	The maximum adsorption percentages	1080:1113	The maximum adsorption percentages of elemental copper, iron, and aluminium	1080:1154	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	17	theme	iron	1136:1139	arg1	%					1166:1166	88.12%	1161:1166	88.12%	1161:1166	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	7	18	theme	CMC	1066:1068	arg1	membrane					1070:1077	a CMC membrane	1064:1077	a CMC membrane	1064:1077	The experimental results showed that the SP content significantly affected the adsorption capacity of a CMC membrane.
30946908	2	19	theme	phytate	283:289	arg1	solution					226:233	A solution	224:233	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP)	224:294	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	1	20	theme	novel	201:205	arg1	membrane					214:221	a novel porous membrane	199:221	a novel porous membrane	199:221	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	8	21	theme	aluminium	1146:1154	arg1	percentages					1103:1113	The maximum adsorption percentages	1080:1113	The maximum adsorption percentages of elemental copper, iron, and aluminium	1080:1154	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	21	theme	aluminium	1146:1154	arg1	%					1166:1166	88.12%	1161:1166	88.12%	1161:1166	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	4	22	theme	membrane	620:627	arg1	content					605:611	the SP content	598:611	the SP content of the membrane, contact time, and contact temperature	598:666	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	4	23	theme	iron	693:696	arg1	adsorption					671:680	adsorption	671:680	adsorption of copper, iron, and aluminium impurities	671:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	1	24	theme	porous	207:212	arg1	membrane					214:221	a novel porous membrane	199:221	a novel porous membrane	199:221	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	5	25	theme	thermogravimetric	759:775	arg1	curves					777:782	thermogravimetric curves	759:782	thermogravimetric curves	759:782	The FTIR spectra and thermogravimetric curves of the membranes indicated good compatibility between CMC and SP.
30946908	6	26	theme	mature	936:941	arg1	structure					951:959	a mature, porous structure	934:959	a mature, porous structure	934:959	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	8	27	theme	elemental	1118:1126	arg1	copper					1128:1133	elemental copper	1118:1133	elemental copper	1118:1133	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	4	28	theme	contact	630:636	arg1	time					638:641	contact time	630:641	contact time	630:641	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	8	29	from	60 °C	1217:1221	arg1	%					1212:1212	80%	1210:1212	80%	1210:1212	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	29	from	60 °C	1217:1221	arg1	ratio					1200:1204	the SP ratio	1193:1204	the SP ratio	1193:1204	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	4	30	theme	content	605:611	arg1	effects					587:593	The effects	583:593	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities	583:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	7	31	theme	adsorption	1041:1050	arg1	capacity					1052:1059	the adsorption capacity	1037:1059	the adsorption capacity of a CMC membrane	1037:1077	The experimental results showed that the SP content significantly affected the adsorption capacity of a CMC membrane.
30946908	3	32	theme	electron	491:498	arg1	SEM					512:514	SEM	512:514	SEM	512:514	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	3	32	theme	electron	491:498	arg1	microscopy					500:509	scanning electron microscopy	482:509	scanning electron microscopy (SEM)	482:515	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	3	33	theme	FTIR	468:471	arg1	spectra					473:479	their FTIR spectra	462:479	their FTIR spectra	462:479	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	3	34	theme	chemical	405:412	arg1	structure					414:422	The chemical structure	401:422	The chemical structure of the membranes	401:439	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	2	35	theme	porous	327:332	arg1	membrane					334:341	a novel porous membrane	319:341	a novel porous membrane	319:341	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	0	36	theme	carboxymethyl-chitosan/sodium	36:64	arg1	characterisation					16:31	characterisation	16:31	characterisation	16:31	Preparation and characterisation of carboxymethyl-chitosan/sodium phytate composite membranes for adsorption in transformer oil.
30946908	0	36	theme	carboxymethyl-chitosan/sodium	36:64	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterisation of carboxymethyl-chitosan/sodium phytate composite membranes for adsorption in transformer oil.
30946908	6	37	theme	composite	910:918	arg1	membranes					920:928	CMC-SP composite membranes	903:928	CMC-SP composite membranes	903:928	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	2	38	theme	O‑carboxymethyl‑chitosan	241:264	arg1	solution					226:233	A solution	224:233	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP)	224:294	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	5	39	theme	good	811:814	arg1	compatibility					816:828	good compatibility	811:828	good compatibility between CMC and SP	811:847	The FTIR spectra and thermogravimetric curves of the membranes indicated good compatibility between CMC and SP.
30946908	5	40	theme	FTIR	742:745	arg1	spectra					747:753	The FTIR spectra	738:753	The FTIR spectra	738:753	The FTIR spectra and thermogravimetric curves of the membranes indicated good compatibility between CMC and SP.
30946908	6	41	theme	CMC-SP	903:908	arg1	membranes					920:928	CMC-SP composite membranes	903:928	CMC-SP composite membranes	903:928	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	7	42	theme	SP	1003:1004	arg1	content					1006:1012	the SP content	999:1012	the SP content	999:1012	The experimental results showed that the SP content significantly affected the adsorption capacity of a CMC membrane.
30946908	3	43	theme	video	556:560	arg1	camera					562:567	video camera	556:567	video camera	556:567	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	1	44	theme	metal	143:147	arg1	impurities					149:158	metal impurities	143:158	metal impurities from transformer oil	143:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	0	45	theme	composite	74:82	arg1	membranes					84:92	composite membranes	74:92	composite membranes for adsorption	74:107	Preparation and characterisation of carboxymethyl-chitosan/sodium phytate composite membranes for adsorption in transformer oil.
30946908	2	46	theme	N	238:238	arg1	solution					226:233	A solution	224:233	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP)	224:294	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	2	47	from	oil	396:398	arg1	adsorption					368:377	the metal impurities adsorption	347:377	the metal impurities adsorption from transformer oil	347:398	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	1	48	theme	impurities	149:158	arg1	Adsorption					129:138	Adsorption	129:138	Adsorption of metal impurities from transformer oil	129:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	1	49	from	Adsorption	129:138	arg1	oil					177:179	transformer oil	165:179	transformer oil	165:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	8	50	theme	maximum	1084:1090	arg1	percentages					1103:1113	The maximum adsorption percentages	1080:1113	The maximum adsorption percentages of elemental copper, iron, and aluminium	1080:1154	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	50	theme	maximum	1084:1090	arg1	%					1166:1166	88.12%	1161:1166	88.12%	1161:1166	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	2	51	theme	sodium	276:281	arg1	SP					292:293	SP	292:293	SP	292:293	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	2	51	theme	sodium	276:281	arg1	phytate					283:289	sodium phytate	276:289	sodium phytate (SP)	276:294	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	6	52	contain	had	930:932	arg1	membranes					920:928	CMC-SP composite membranes	903:928	CMC-SP composite membranes	903:928	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	6	52	contain	had	930:932	arg2	structure					951:959	a mature, porous structure	934:959	a mature, porous structure	934:959	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	2	53	theme	novel	321:325	arg1	membrane					334:341	a novel porous membrane	319:341	a novel porous membrane	319:341	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	8	54	theme	SP	1197:1198	arg1	%					1212:1212	80%	1210:1212	80%	1210:1212	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	54	theme	SP	1197:1198	arg1	ratio					1200:1204	the SP ratio	1193:1204	the SP ratio	1193:1204	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	3	55	theme	thermogravimetric	518:534	arg1	analysis					536:543	thermogravimetric analysis	518:543	thermogravimetric analysis (TGA)	518:549	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	3	55	theme	thermogravimetric	518:534	arg1	TGA					546:548	TGA	546:548	TGA	546:548	The chemical structure of the membranes was characterised by their FTIR spectra, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and video camera observations.
30946908	6	56	dep	mature	936:941	arg1	porous					944:949	porous	944:949	porous	944:949	The SEM and video camera observations suggested that CMC-SP composite membranes had a mature, porous structure.
30946908	4	57	theme	impurities	713:722	arg1	adsorption					671:680	adsorption	671:680	adsorption of copper, iron, and aluminium impurities	671:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	1	58	theme	transformer	165:175	arg1	oil					177:179	transformer oil	165:179	transformer oil	165:179	Adsorption of metal impurities from transformer oil was studied using a novel porous membrane.
30946908	4	59	theme	copper	685:690	arg1	adsorption					671:680	adsorption	671:680	adsorption of copper, iron, and aluminium impurities	671:722	The effects of the SP content of the membrane, contact time, and contact temperature on adsorption of copper, iron, and aluminium impurities were studied.
30946908	7	60	theme	experimental	966:977	arg1	results					979:985	The experimental results	962:985	The experimental results	962:985	The experimental results showed that the SP content significantly affected the adsorption capacity of a CMC membrane.
30946908	2	61	theme	impurities	357:366	arg1	adsorption					368:377	the metal impurities adsorption	347:377	the metal impurities adsorption from transformer oil	347:398	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	2	62	theme	transformer	384:394	arg1	oil					396:398	transformer oil	384:398	transformer oil	384:398	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
30946908	8	63	theme	adsorption	1092:1101	arg1	percentages					1103:1113	The maximum adsorption percentages	1080:1113	The maximum adsorption percentages of elemental copper, iron, and aluminium	1080:1154	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	8	63	theme	adsorption	1092:1101	arg1	%					1166:1166	88.12%	1161:1166	88.12%	1161:1166	The maximum adsorption percentages of elemental copper, iron, and aluminium were 88.12%, 82.35%, and 80.36% when the SP ratio was 80% at 60 °C.
30946908	2	64	theme	metal	351:355	arg1	adsorption					368:377	the metal impurities adsorption	347:377	the metal impurities adsorption from transformer oil	347:398	A solution of N, O‑carboxymethyl‑chitosan (CMC) and sodium phytate (SP) was blended to prepare a novel porous membrane for the metal impurities adsorption from transformer oil.
31654949	4	0	theme	sensory	445:451	arg1	quality					453:459	sensory quality	445:459	sensory quality	445:459	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	7	1	dep	affected	779:786	arg1	whereas					801:807	whereas	801:807	whereas	801:807	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	4	2	theme	health-related	462:475	arg1	compounds					477:485	health-related compounds	462:485	health-related compounds (e.g. acrylamide, β-glucan)	462:513	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	5	3	theme	oat	579:581	arg1	flakes					583:588	oat flakes	579:588	oat flakes with improved sensory properties	579:621	Roasting resulted in oat flakes with improved sensory properties.
31654949	7	4	theme	dietary	809:815	arg1	fractions					823:831	dietary fibre fractions	809:831	dietary fibre fractions	809:831	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	0	5	theme	products	98:105	arg1	compounds					81:89	health-related compounds	66:89	health-related compounds	66:89	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	0	5	theme	products	98:105	arg1	properties					51:60	sensory properties	43:60	sensory properties	43:60	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	0	6	from	Impact	0:5	arg1	compounds					81:89	health-related compounds	66:89	health-related compounds	66:89	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	0	6	from	Impact	0:5	arg1	properties					51:60	sensory properties	43:60	sensory properties	43:60	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	5	7	theme	sensory	604:610	arg1	properties					612:621	improved sensory properties	595:621	improved sensory properties	595:621	Roasting resulted in oat flakes with improved sensory properties.
31654949	7	8	theme	fibre	817:821	arg1	fractions					823:831	dietary fibre fractions	809:831	dietary fibre fractions	809:831	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	4	9	theme	kernels	538:544	arg1	quality					453:459	sensory quality	445:459	sensory quality	445:459	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	9	theme	kernels	538:544	arg1	compounds					477:485	health-related compounds	462:485	health-related compounds (e.g. acrylamide, β-glucan)	462:513	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	9	theme	kernels	538:544	arg1	viscosities					519:529	viscosities	519:529	viscosities of oat kernels and flakes	519:555	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	0	10	theme	oat	94:96	arg1	products					98:105	oat products	94:105	oat products	94:105	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	6	11	theme	Acrylamide	624:633	arg1	formation					635:643	Acrylamide formation	624:643	Acrylamide formation	624:643	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	9	12	theme	oat	1039:1041	arg1	products					1043:1050	oat products	1039:1050	oat products	1039:1050	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	4	13	theme	oat	534:536	arg1	kernels					538:544	oat kernels	534:544	oat kernels	534:544	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	9	14	with	technique	1002:1010	arg1	potential					1017:1025	potential	1017:1025	potential	1017:1025	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	6	15	theme	roasting	667:674	arg1	temperatures					676:687	higher roasting temperatures	660:687	higher roasting temperatures	660:687	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	3	16	theme	roasting	393:400	arg1	conditions					402:411	different roasting conditions	383:411	different roasting conditions	383:411	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	9	17	theme	processing	991:1000	arg1	up					973:974	roasting up	964:974	roasting up to 160 °C	964:984	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	9	17	theme	processing	991:1000	arg1	technique					1002:1010	a processing technique	989:1010	a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition	989:1119	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	3	18	dep	analyse	361:367	arg1	140-180 °C					414:423	140-180 °C	414:423	140-180 °C	414:423	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	6	19	theme	higher	660:665	arg1	temperatures					676:687	higher roasting temperatures	660:687	higher roasting temperatures	660:687	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	3	20	theme	conditions	402:411	arg1	impact					373:378	the impact	369:378	the impact of different roasting conditions	369:411	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	3	21	theme	study	348:352	arg1	aim					329:331	the aim	325:331	the aim of the present study	325:352	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	4	22	from	20 min	434:439	arg1	quality					453:459	sensory quality	445:459	sensory quality	445:459	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	22	from	20 min	434:439	arg1	viscosities					519:529	viscosities	519:529	viscosities of oat kernels and flakes	519:555	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	22	from	20 min	434:439	arg1	compounds					477:485	health-related compounds	462:485	health-related compounds (e.g. acrylamide, β-glucan)	462:513	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	9	23	theme	improved	1057:1064	arg1	quality					1074:1080	improved sensory quality	1057:1080	improved sensory quality	1057:1080	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	1	24	theme	oat	166:168	arg1	products					170:177	oat products	166:177	oat products	166:177	Due to their high content of β-glucan, the consumption of oat products can contribute to a healthy diet.
31654949	8	25	theme	roasting	915:922	arg1	temperatures					924:935	roasting temperatures	915:935	roasting temperatures	915:935	Viscosities were significantly reduced with increasing roasting temperatures.
31654949	7	26	theme	starch	750:755	arg1	Contents					724:731	Contents	724:731	Contents of fat, protein, starch and β-glucan	724:768	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	9	27	theme	sensory	1066:1072	arg1	quality					1074:1080	improved sensory quality	1057:1080	improved sensory quality	1057:1080	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	3	28	theme	different	383:391	arg1	conditions					402:411	different roasting conditions	383:411	different roasting conditions	383:411	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	0	29	theme	roasting	20:27	arg1	conditions					29:38	different roasting conditions	10:38	different roasting conditions	10:38	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	2	30	theme	nutritional	279:289	arg1	value					291:295	the nutritional value	275:295	the nutritional value of oat products	275:311	Roasting may improve sensory properties but could also affect the nutritional value of oat products.
31654949	3	31	theme	present	340:346	arg1	study					348:352	the present study	336:352	the present study	336:352	Therefore, the aim of the present study was to analyse the impact of different roasting conditions (140-180 °C, approx.
31654949	7	32	theme	protein	741:747	arg1	Contents					724:731	Contents	724:731	Contents of fat, protein, starch and β-glucan	724:768	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	4	33	theme	flakes	550:555	arg1	quality					453:459	sensory quality	445:459	sensory quality	445:459	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	33	theme	flakes	550:555	arg1	compounds					477:485	health-related compounds	462:485	health-related compounds (e.g. acrylamide, β-glucan)	462:513	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	33	theme	flakes	550:555	arg1	viscosities					519:529	viscosities	519:529	viscosities of oat kernels and flakes	519:555	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	9	34	theme	favourable	1086:1095	arg1	composition					1109:1119	favourable nutritional composition	1086:1119	favourable nutritional composition	1086:1119	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	5	35	theme	improved	595:602	arg1	properties					612:621	improved sensory properties	595:621	improved sensory properties	595:621	Roasting resulted in oat flakes with improved sensory properties.
31654949	9	36	theme	roasting	964:971	arg1	up					973:974	roasting up	964:974	roasting up to 160 °C	964:984	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	9	36	theme	roasting	964:971	arg1	technique					1002:1010	a processing technique	989:1010	a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition	989:1119	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	1	37	theme	products	170:177	arg1	consumption					151:161	the consumption	147:161	the consumption of oat products	147:177	Due to their high content of β-glucan, the consumption of oat products can contribute to a healthy diet.
31654949	0	38	theme	conditions	29:38	arg1	Impact					0:5	Impact	0:5	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.	0:106	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	7	39	theme	fat	736:738	arg1	Contents					724:731	Contents	724:731	Contents of fat, protein, starch and β-glucan	724:768	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	9	40	theme	nutritional	1097:1107	arg1	composition					1109:1119	favourable nutritional composition	1086:1119	favourable nutritional composition	1086:1119	The results indicate that roasting up to 160 °C is a processing technique with potential to generate oat products with improved sensory quality and favourable nutritional composition.
31654949	0	41	theme	sensory	43:49	arg1	properties					51:60	sensory properties	43:60	sensory properties	43:60	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	6	42	theme	thick	710:714	arg1	flakes					716:721	thin and thick flakes	701:721	thin and thick flakes	701:721	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	6	42	theme	thick	710:714	arg1	kernels					692:698	kernels	692:698	kernels	692:698	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	5	43	with	flakes	583:588	arg1	properties					612:621	improved sensory properties	595:621	improved sensory properties	595:621	Roasting resulted in oat flakes with improved sensory properties.
31654949	1	44	theme	high	121:124	arg1	content					126:132	their high content	115:132	their high content of β-glucan	115:144	Due to their high content of β-glucan, the consumption of oat products can contribute to a healthy diet.
31654949	1	45	theme	healthy	199:205	arg1	diet					207:210	a healthy diet	197:210	a healthy diet	197:210	Due to their high content of β-glucan, the consumption of oat products can contribute to a healthy diet.
31654949	2	46	theme	sensory	234:240	arg1	properties					242:251	sensory properties	234:251	sensory properties	234:251	Roasting may improve sensory properties but could also affect the nutritional value of oat products.
31654949	6	47	theme	thin	701:704	arg1	flakes					716:721	thin and thick flakes	701:721	thin and thick flakes	701:721	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	6	47	theme	thin	701:704	arg1	kernels					692:698	kernels	692:698	kernels	692:698	Acrylamide formation increased with higher roasting temperatures in kernels, thin and thick flakes.
31654949	2	48	theme	oat	300:302	arg1	products					304:311	oat products	300:311	oat products	300:311	Roasting may improve sensory properties but could also affect the nutritional value of oat products.
31654949	7	49	theme	β-glucan	761:768	arg1	Contents					724:731	Contents	724:731	Contents of fat, protein, starch and β-glucan	724:768	Contents of fat, protein, starch and β-glucan were not affected by roasting, whereas dietary fibre fractions were marginally modulated.
31654949	2	50	theme	products	304:311	arg1	value					291:295	the nutritional value	275:295	the nutritional value of oat products	275:311	Roasting may improve sensory properties but could also affect the nutritional value of oat products.
31654949	0	51	theme	health-related	66:79	arg1	compounds					81:89	health-related compounds	66:89	health-related compounds	66:89	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	4	52	dep	acrylamide	493:502	arg1	e.g.					488:491	e.g.	488:491	e.g.	488:491	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	4	52	dep	acrylamide	493:502	arg1	β-glucan					505:512	β-glucan	505:512	β-glucan	505:512	20 min) on sensory quality, health-related compounds (e.g. acrylamide, β-glucan) and viscosities of oat kernels and flakes.
31654949	0	53	theme	different	10:18	arg1	conditions					29:38	different roasting conditions	10:38	different roasting conditions	10:38	Impact of different roasting conditions on sensory properties and health-related compounds of oat products.
31654949	1	54	theme	β-glucan	137:144	arg1	content					126:132	their high content	115:132	their high content of β-glucan	115:144	Due to their high content of β-glucan, the consumption of oat products can contribute to a healthy diet.
30335947	3	0	link	cross-linked	603:614	arg1	alginate					616:623	ionically cross-linked alginate	593:623	ionically cross-linked alginate	593:623	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	5	1	theme	interfacial	829:839	arg1	propagation					847:857	interfacial crack propagation	829:857	interfacial crack propagation	829:857	Instead, mechanistic principles were used to control interfacial crack propagation.
30335947	5	2	used	used	813:816	arg2	principles					797:806	mechanistic principles	785:806	mechanistic principles	785:806	Instead, mechanistic principles were used to control interfacial crack propagation.
30335947	4	3	theme	developed	756:764	arg1	hydrogel					766:773	the developed hydrogel	752:773	the developed hydrogel	752:773	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
30335947	5	4	theme	crack	841:845	arg1	propagation					847:857	interfacial crack propagation	829:857	interfacial crack propagation	829:857	Instead, mechanistic principles were used to control interfacial crack propagation.
30335947	3	5	theme	cross-linked	539:550	arg1	dimethacrylate					574:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	6	6	theme	articular	1061:1069	arg1	cartilage					1071:1079	articular cartilage	1061:1079	articular cartilage	1061:1079	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	5	7	theme	mechanistic	785:795	arg1	principles					797:806	mechanistic principles	785:806	mechanistic principles	785:806	Instead, mechanistic principles were used to control interfacial crack propagation.
30335947	2	8	with	hydrogel	351:358	arg1	content					376:382	∼90% water content	365:382	∼90% water content	365:382	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	6	9	theme	tissue	884:889	arg1	adhesives					891:899	commercial tissue adhesives	873:899	commercial tissue adhesives	873:899	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	1	10	theme	high	121:124	arg1	properties					137:146	high mechanical properties	121:146	high mechanical properties	121:146	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	4	11	theme	surface	682:688	arg1	modification					690:701	No tissue surface modification	672:701	No tissue surface modification	672:701	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
30335947	3	12	theme	ethylene	557:564	arg1	poly					552:555	poly	552:555	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	3	12	theme	ethylene	557:564	arg1	glycol					566:571	ethylene glycol	557:571	ethylene glycol	557:571	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	2	13	theme	dissipative	401:411	arg1	interface					413:421	a dissipative interface	399:421	a dissipative interface	399:421	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	0	14	from	Adhesion	46:53	arg1	Surfaces					69:76	Biological Surfaces	58:76	Biological Surfaces	58:76	Composite Double-Network Hydrogels To Improve Adhesion on Biological Surfaces.
30335947	8	15	theme	tissues	1308:1314	arg1	repair					1316:1321	tissues repair	1308:1321	tissues repair	1308:1321	This research provides a promising path to the development of highly adhesive hydrogels for tissues repair.
30335947	0	16	theme	Double-Network	10:23	arg1	Hydrogels					25:33	Composite Double-Network Hydrogels	0:33	Composite Double-Network Hydrogels	0:33	Composite Double-Network Hydrogels To Improve Adhesion on Biological Surfaces.
30335947	7	17	theme	dissipation	1164:1174	arg1	processes					1176:1184	dissipation processes	1164:1184	dissipation processes	1164:1184	Our findings highlight the significant role of controlling hydrogel structure and dissipation processes for toughening the interface.
30335947	3	18	theme	cross-linked	603:614	arg1	alginate					616:623	ionically cross-linked alginate	593:623	ionically cross-linked alginate	593:623	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	6	19	theme	tissue	967:972	arg1	surfaces					974:981	the soft tissue surfaces	958:981	the soft tissue surfaces	958:981	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	1	20	theme	biological	199:208	arg1	surfaces					210:217	biological surfaces	199:217	biological surfaces	199:217	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	0	21	theme	Composite	0:8	arg1	Hydrogels					25:33	Composite Double-Network Hydrogels	0:33	Composite Double-Network Hydrogels	0:33	Composite Double-Network Hydrogels To Improve Adhesion on Biological Surfaces.
30335947	2	22	theme	double-network	336:349	arg1	hydrogel					351:358	a composite double-network hydrogel	324:358	a composite double-network hydrogel	324:358	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	8	23	theme	promising	1241:1249	arg1	path					1251:1254	a promising path	1239:1254	a promising path to the development of highly adhesive hydrogels for tissues repair	1239:1321	This research provides a promising path to the development of highly adhesive hydrogels for tissues repair.
30335947	4	24	theme	high	724:727	arg1	properties					738:747	high adhesion properties	724:747	high adhesion properties of the developed hydrogel	724:773	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
30335947	8	25	theme	hydrogels	1294:1302	arg1	development					1263:1273	the development	1259:1273	the development of highly adhesive hydrogels for tissues repair	1259:1321	This research provides a promising path to the development of highly adhesive hydrogels for tissues repair.
30335947	6	26	theme	commercial	873:882	arg1	adhesives					891:899	commercial tissue adhesives	873:899	commercial tissue adhesives	873:899	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	2	27	theme	composite	326:334	arg1	hydrogel					351:358	a composite double-network hydrogel	324:358	a composite double-network hydrogel	324:358	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	2	28	theme	soft	447:450	arg1	meniscus					482:489	meniscus	482:489	meniscus	482:489	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	2	28	theme	soft	447:450	arg1	tissues					452:458	soft tissues	447:458	soft tissues such as cartilage and meniscus	447:489	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	2	28	theme	soft	447:450	arg1	cartilage					468:476	cartilage	468:476	cartilage	468:476	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	8	29	theme	adhesive	1285:1292	arg1	hydrogels					1294:1302	highly adhesive hydrogels	1278:1302	highly adhesive hydrogels for tissues repair	1278:1321	This research provides a promising path to the development of highly adhesive hydrogels for tissues repair.
30335947	3	30	link	cross-linked	539:550	arg1	dimethacrylate					574:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	6	31	theme	significant	993:1003	arg1	increase					1005:1012	a significant increase	991:1012	a significant increase in the adhesion strength	991:1037	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	3	32	theme	poly	552:555	arg1	dimethacrylate					574:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	covalently cross-linked poly(ethylene glycol) dimethacrylate	528:587	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	6	33	from	integration	906:916	arg1	surfaces					974:981	the soft tissue surfaces	958:981	the soft tissue surfaces	958:981	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	2	34	theme	%	368:368	arg1	content					376:382	∼90% water content	365:382	∼90% water content	365:382	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	6	35	theme	network	947:953	arg1	integration					906:916	the integration	902:916	the integration of the dissipative polymeric network on the soft tissue surfaces	902:981	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	6	36	from	increase	1005:1012	arg1	strength					1030:1037	the adhesion strength	1017:1037	the adhesion strength	1017:1037	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	7	37	theme	hydrogel	1141:1148	arg1	structure					1150:1158	hydrogel structure	1141:1158	hydrogel structure	1141:1158	Our findings highlight the significant role of controlling hydrogel structure and dissipation processes for toughening the interface.
30335947	6	38	theme	polymeric	937:945	arg1	network					947:953	the dissipative polymeric network	921:953	the dissipative polymeric network	921:953	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	3	39	theme	nanofibrillated	645:659	arg1	cellulose					661:669	nanofibrillated cellulose	645:669	nanofibrillated cellulose	645:669	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	0	40	theme	Biological	58:67	arg1	Surfaces					69:76	Biological Surfaces	58:76	Biological Surfaces	58:76	Composite Double-Network Hydrogels To Improve Adhesion on Biological Surfaces.
30335947	4	41	theme	adhesion	729:736	arg1	properties					738:747	high adhesion properties	724:747	high adhesion properties of the developed hydrogel	724:773	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
30335947	6	42	theme	dissipative	925:935	arg1	network					947:953	the dissipative polymeric network	921:953	the dissipative polymeric network	921:953	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	1	43	dep	materials	185:193	arg1	these					179:183	these	179:183	these	179:183	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	1	44	from	use	246:248	arg1	field					268:272	the biomedical field	253:272	the biomedical field	253:272	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	2	45	theme	water	370:374	arg1	content					376:382	∼90% water content	365:382	∼90% water content	365:382	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	6	46	dep	such	1040:1043	arg1	as					1045:1046	as	1045:1046	as	1045:1046	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	1	47	theme	mechanical	126:135	arg1	properties					137:146	high mechanical properties	121:146	high mechanical properties	121:146	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	2	48	theme	toughening	290:299	arg1	processes					301:309	toughening processes	290:309	toughening processes	290:309	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	2	49	theme	∼90	365:367	arg1	%					368:368	%	368:368	%	368:368	By controlling toughening processes, we designed a composite double-network hydrogel with ∼90% water content, which creates a dissipative interface and robustly adheres to soft tissues such as cartilage and meniscus.
30335947	1	50	theme	hydrogels	106:114	arg1	development					91:101	the development	87:101	the development of hydrogels with high mechanical properties	87:146	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	7	51	theme	significant	1109:1119	arg1	role					1121:1124	the significant role	1105:1124	the significant role of controlling hydrogel structure and dissipation processes for toughening the interface	1105:1213	Our findings highlight the significant role of controlling hydrogel structure and dissipation processes for toughening the interface.
30335947	3	52	theme	double-network	494:507	arg1	matrix					509:514	A double-network matrix	492:514	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate	492:623	A double-network matrix composed of covalently cross-linked poly(ethylene glycol) dimethacrylate and ionically cross-linked alginate was reinforced with nanofibrillated cellulose.
30335947	6	53	theme	soft	962:965	arg1	surfaces					974:981	the soft tissue surfaces	958:981	the soft tissue surfaces	958:981	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	4	54	theme	tissue	675:680	arg1	modification					690:701	No tissue surface modification	672:701	No tissue surface modification	672:701	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
30335947	1	55	theme	insufficient	149:160	arg1	adhesion					162:169	insufficient adhesion	149:169	insufficient adhesion between these materials and biological surfaces	149:217	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	1	56	theme	biomedical	257:266	arg1	field					268:272	the biomedical field	253:272	the biomedical field	253:272	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	1	57	with	hydrogels	106:114	arg1	properties					137:146	high mechanical properties	121:146	high mechanical properties	121:146	Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field.
30335947	6	58	theme	adhesion	1021:1028	arg1	strength					1030:1037	the adhesion strength	1017:1037	the adhesion strength	1017:1037	Comparing to commercial tissue adhesives, the integration of the dissipative polymeric network on the soft tissue surfaces allowed a significant increase in the adhesion strength, such as ∼130 kPa for articular cartilage.
30335947	4	59	theme	hydrogel	766:773	arg1	properties					738:747	high adhesion properties	724:747	high adhesion properties of the developed hydrogel	724:773	No tissue surface modification was needed to obtain high adhesion properties of the developed hydrogel.
31760008	4	0	theme	formation	911:919	arg1	mechanism					921:929	The formation mechanism	907:929	The formation mechanism of the composite fiber	907:952	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	1	from	PEC	669:671	arg1	solution					735:742	NaCl solution	730:742	NaCl solution	730:742	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	2	1	from	PEC	669:671	arg1	water					720:724	pure water	715:724	pure water	715:724	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	3	2	theme	as-prepared	767:777	arg1	fibers					779:784	the as-prepared fibers	763:784	the as-prepared fibers	763:784	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	7	3	theme	drying	1529:1534	arg1	techniques					1536:1545	PEC and freeze drying techniques	1514:1545	PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte	1514:1602	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	2	4	theme	drying	685:690	arg1	method					700:705	polyelectrolyte complexation (PEC) and freeze drying coupled method	639:705	method	700:705	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	6	5	theme	complexing	1271:1280	arg1	system					1282:1287	the complexing system	1267:1287	the complexing system	1267:1287	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	3	6	theme	NaCl	805:808	arg1	concentration					810:822	NaCl concentration	805:822	NaCl concentration	805:822	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	7	7	with	NaCl	1553:1556	arg1	concentration					1571:1583	desired concentration	1563:1583	desired concentration	1563:1583	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	1	8	theme	complicated	327:337	arg1	processes					351:359	complicated preparation processes	327:359	complicated preparation processes	327:359	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	5	9	theme	composite	1176:1184	arg1	fiber					1186:1190	the composite fiber	1172:1190	the composite fiber	1172:1190	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	1	10	theme	preparation	339:349	arg1	processes					351:359	complicated preparation processes	327:359	complicated preparation processes	327:359	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	7	11	theme	controllable	1453:1464	arg1	structures					1466:1475	controllable structures	1453:1475	controllable structures	1453:1475	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	1	12	theme	electrospinning	235:249	arg1	process					251:257	The conventional electrospinning process	218:257	The conventional electrospinning process for the preparation of fibers	218:287	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	2	13	theme	complexation	655:666	arg1	PEC					669:671	polyelectrolyte complexation (PEC) and freeze drying coupled method	639:705	PEC	669:671	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	7	14	theme	polymers	1439:1446	arg1	route					1398:1402	a new preparation route	1380:1402	a new preparation route to the composite fibers of natural polymers with controllable structures and properties	1380:1490	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	0	15	theme	ammonium	137:144	arg1	chloride					146:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	84:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	84:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	5	16	theme	swelling	1110:1117	arg1	properties					1119:1128	The swelling properties	1106:1128	The swelling properties	1106:1128	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	4	17	theme	NaCl	973:976	arg1	concentration					978:990	NaCl concentration	973:990	NaCl concentration	973:990	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	0	18	theme	chitosan	155:162	arg1	fiber					75:79	composite fiber	65:79	composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	65:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	4	19	from	effects	962:968	arg1	structure					995:1003	structure	995:1003	structure	995:1003	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	19	from	effects	962:968	arg1	properties					1009:1018	properties	1009:1018	properties	1009:1018	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	20	theme	composite	938:946	arg1	fiber					948:952	the composite fiber	934:952	the composite fiber	934:952	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	7	21	theme	composite	1411:1419	arg1	fibers					1421:1426	the composite fibers	1407:1426	the composite fibers	1407:1426	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	3	22	theme	fibers	845:850	arg1	structures					831:840	the structures	827:840	the structures of fibers	827:850	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	1	23	theme	biomedical	468:477	arg1	materials					479:487	biomedical materials	468:487	biomedical materials	468:487	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	3	24	theme	solid	874:878	arg1	NMR					884:886	solid 13C NMR	874:886	solid 13C NMR	874:886	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	6	25	theme	fiber	1351:1355	arg1	structure					1315:1323	structure	1315:1323	structure	1315:1323	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	6	25	theme	fiber	1351:1355	arg1	properties					1329:1338	properties	1329:1338	properties	1329:1338	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	7	26	theme	preparation	1386:1396	arg1	route					1398:1402	a new preparation route	1380:1402	a new preparation route to the composite fibers of natural polymers with controllable structures and properties	1380:1490	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	2	27	theme	chitosan	583:590	arg1	fibers					613:618	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	0	28	theme	composite	65:73	arg1	fiber					75:79	composite fiber	65:79	composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	65:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	5	29	theme	fiber	1186:1190	arg1	kinetics					1160:1167	the thermal decomposition kinetics	1134:1167	the thermal decomposition kinetics	1134:1167	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	5	29	theme	fiber	1186:1190	arg1	properties					1119:1128	The swelling properties	1106:1128	The swelling properties	1106:1128	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	0	30	from	Effects	0:6	arg1	properties					51:60	properties	51:60	properties	51:60	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	0	30	from	Effects	0:6	arg1	structure					37:45	structure	37:45	structure	37:45	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	5	31	theme	thermal	1138:1144	arg1	kinetics					1160:1167	the thermal decomposition kinetics	1134:1167	the thermal decomposition kinetics	1134:1167	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	2	32	theme	composite	603:611	arg1	fibers					613:618	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	0	33	theme	cellulose/N-2-hydroxylpropyl	98:125	arg1	chloride					146:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	84:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	84:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	7	34	theme	new	1382:1384	arg1	route					1398:1402	a new preparation route	1380:1402	a new preparation route to the composite fibers of natural polymers with controllable structures and properties	1380:1490	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	4	35	theme	Materials	1065:1073	arg1	software					1082:1089	the Materials Studio software	1061:1089	the Materials Studio software	1061:1089	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	1	36	from	application	434:444	arg1	preparation					453:463	the preparation	449:463	the preparation of biomedical materials	449:487	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	4	37	from	software	1082:1089	arg1	simulated					1048:1056	simulated	1048:1056	simulated	1048:1056	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	38	theme	current	497:503	arg1	study					505:509	the current study	493:509	the current study	493:509	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	6	39	theme	NaCl	1247:1250	arg1	electrolyte					1252:1262	NaCl electrolyte	1247:1262	NaCl electrolyte	1247:1262	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	0	40	theme	salt	11:14	arg1	concentration					16:28	salt concentration	11:28	salt concentration	11:28	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	4	41	from	mechanism	921:929	arg1	structure					995:1003	structure	995:1003	structure	995:1003	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	41	from	mechanism	921:929	arg1	properties					1009:1018	properties	1009:1018	properties	1009:1018	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	42	theme	ammonium	565:572	arg1	chloride					574:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	512:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	4	43	theme	fiber	1037:1041	arg1	structure					995:1003	structure	995:1003	structure	995:1003	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	43	theme	fiber	1037:1041	arg1	properties					1009:1018	properties	1009:1018	properties	1009:1018	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	0	44	dep	structure	37:45	arg1	the					33:35	the	33:35	the	33:35	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	2	45	theme	cellulose/N-2-hydroxylpropyl	526:553	arg1	chloride					574:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	512:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	1	46	theme	chemical	373:380	arg1	crosslinkers					382:393	chemical crosslinkers	373:393	chemical crosslinkers	373:393	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	7	47	theme	desired	1563:1569	arg1	concentration					1571:1583	desired concentration	1563:1583	desired concentration	1563:1583	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	7	48	theme	techniques	1536:1545	arg1	combination					1499:1509	the combination	1495:1509	the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte	1495:1602	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	4	49	theme	composite	1027:1035	arg1	fiber					1037:1041	the composite fiber	1023:1041	the composite fiber	1023:1041	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	6	50	theme	PEC	1347:1349	arg1	fiber					1351:1355	the PEC fiber	1343:1355	the PEC fiber	1343:1355	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	1	51	theme	complex	305:311	arg1	equipment					313:321	complex equipment	305:321	complex equipment	305:321	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	6	52	dep	structure	1315:1323	arg1	the					1311:1313	the	1311:1313	the	1311:1313	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	7	53	theme	freeze	1522:1527	arg1	drying					1529:1534	freeze drying	1522:1534	freeze drying	1522:1534	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	2	54	theme	coupled	692:698	arg1	method					700:705	polyelectrolyte complexation (PEC) and freeze drying coupled method	639:705	method	700:705	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	3	55	theme	fibers	779:784	arg1	structures					749:758	The structures	745:758	The structures of the as-prepared fibers	745:784	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	3	55	theme	fibers	779:784	arg1	effects					794:800	the effects	790:800	the effects of NaCl concentration on the structures of fibers	790:850	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	7	56	theme	PEC	1514:1516	arg1	techniques					1536:1545	PEC and freeze drying techniques	1514:1545	PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte	1514:1602	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	2	57	theme	freeze	678:683	arg1	method					700:705	polyelectrolyte complexation (PEC) and freeze drying coupled method	639:705	method	700:705	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	3	58	theme	concentration	810:822	arg1	structures					749:758	The structures	745:758	The structures of the as-prepared fibers	745:784	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	3	58	theme	concentration	810:822	arg1	effects					794:800	the effects	790:800	the effects of NaCl concentration on the structures of fibers	790:850	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	2	59	theme	pure	715:718	arg1	water					720:724	pure water	715:724	pure water	715:724	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	3	60	from	effects	794:800	arg1	structures					831:840	the structures	827:840	the structures of fibers	827:850	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	1	61	theme	conventional	222:233	arg1	process					251:257	The conventional electrospinning process	218:257	The conventional electrospinning process for the preparation of fibers	218:287	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	0	62	theme	chloride	146:153	arg1	chitosan					155:162	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan	84:162	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	84:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	2	63	theme	polyelectrolyte	639:653	arg1	PEC					669:671	polyelectrolyte complexation (PEC) and freeze drying coupled method	639:705	PEC	669:671	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	7	64	theme	natural	1431:1437	arg1	polymers					1439:1446	natural polymers	1431:1446	natural polymers with controllable structures and properties	1431:1490	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	1	65	theme	materials	479:487	arg1	preparation					453:463	the preparation	449:463	the preparation of biomedical materials	449:487	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	4	66	theme	fiber	948:952	arg1	effects					962:968	the effects	958:968	the effects of NaCl concentration on structure and properties of the composite fiber	958:1041	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	66	theme	fiber	948:952	arg1	mechanism					921:929	The formation mechanism	907:929	The formation mechanism of the composite fiber	907:952	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	6	67	theme	electrolyte	1252:1262	arg1	addition					1235:1242	the addition	1231:1242	the addition of NaCl electrolyte to the complexing system	1231:1287	The results suggest that the addition of NaCl electrolyte to the complexing system significantly affects the structure and properties of the PEC fiber.
31760008	3	68	theme	13C	880:882	arg1	NMR					884:886	solid 13C NMR	874:886	solid 13C NMR	874:886	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
31760008	0	69	theme	fiber	75:79	arg1	properties					51:60	properties	51:60	properties	51:60	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	0	69	theme	fiber	75:79	arg1	structure					37:45	structure	37:45	structure	37:45	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	1	70	theme	organic	399:405	arg1	solvents					407:414	organic solvents	399:414	organic solvents	399:414	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	2	71	theme	chloride	574:581	arg1	CMC/HACC					593:600	CMC/HACC	593:600	CMC/HACC	593:600	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	2	71	theme	chloride	574:581	arg1	chitosan					583:590	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan	512:590	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	0	72	theme	carboxymethyl	84:96	arg1	chloride					146:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	84:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	84:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	4	73	theme	concentration	978:990	arg1	effects					962:968	the effects	958:968	the effects of NaCl concentration on structure and properties of the composite fiber	958:1041	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	4	73	theme	concentration	978:990	arg1	mechanism					921:929	The formation mechanism	907:929	The formation mechanism of the composite fiber	907:952	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	74	theme	NaCl	730:733	arg1	solution					735:742	NaCl solution	730:742	NaCl solution	730:742	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	0	75	theme	trimethyl	127:135	arg1	chloride					146:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	84:153	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	84:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	5	76	theme	decomposition	1146:1158	arg1	kinetics					1160:1167	the thermal decomposition kinetics	1134:1167	the thermal decomposition kinetics	1134:1167	The swelling properties and the thermal decomposition kinetics of the composite fiber were studied.
31760008	7	77	with	polymers	1439:1446	arg1	properties					1481:1490	properties	1481:1490	properties	1481:1490	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	7	77	with	polymers	1439:1446	arg1	structures					1466:1475	controllable structures	1453:1475	controllable structures	1453:1475	Our work has provided a new preparation route to the composite fibers of natural polymers with controllable structures and properties by the combination of PEC and freeze drying techniques using NaCl with desired concentration as the electrolyte.
31760008	0	78	theme	concentration	16:28	arg1	Effects					0:6	Effects	0:6	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte	0:188	Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying.
31760008	4	79	theme	Studio	1075:1080	arg1	software					1082:1089	the Materials Studio software	1061:1089	the Materials Studio software	1061:1089	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	80	from	method	700:705	arg1	solution					735:742	NaCl solution	730:742	NaCl solution	730:742	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	2	80	from	method	700:705	arg1	water					720:724	pure water	715:724	pure water	715:724	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	2	81	theme	trimethyl	555:563	arg1	chloride					574:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	512:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	4	82	from	simulated	1048:1056	arg1	software					1082:1089	the Materials Studio software	1061:1089	the Materials Studio software	1061:1089	The formation mechanism of the composite fiber and the effects of NaCl concentration on structure and properties of the composite fiber were simulated in the Materials Studio software and discussed.
31760008	2	83	theme	carboxymethyl	512:524	arg1	chloride					574:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride	512:581	carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers	512:618	In the current study, carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan (CMC/HACC) composite fibers were fabricated by polyelectrolyte complexation (PEC) and freeze drying coupled method in both pure water and NaCl solution.
31760008	1	84	theme	fibers	282:287	arg1	preparation					267:277	the preparation	263:277	the preparation of fibers	263:287	The conventional electrospinning process for the preparation of fibers usually require complex equipment and complicated preparation processes, as well as chemical crosslinkers and organic solvents, which limits its application in the preparation of biomedical materials.
31760008	3	85	from	structures	749:758	arg1	structures					831:840	the structures	827:840	the structures of fibers	827:850	The structures of the as-prepared fibers and the effects of NaCl concentration on the structures of fibers were studied by FTIR, solid 13C NMR, XRD, XPS and SEM.
29315387	0	0	theme	genome	84:89	arg1	targeting					41:49	CRISPR/Cas9 targeting	29:49	CRISPR/Cas9 targeting of the human glycosyltransferase genome	29:89	A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome.
29315387	4	1	theme	genetic	952:958	arg1	dissection					960:969	genetic dissection	952:969	genetic dissection of biosynthetic pathways	952:994	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	4	2	theme	CRISPR/Cas9	836:846	arg1	tool					861:864	the facile CRISPR/Cas9 gene editing tool	825:864	the facile CRISPR/Cas9 gene editing tool	825:864	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	2	3	from	roles	408:412	arg1	proteins					546:553	proteins	546:553	proteins	546:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	4	4	theme	direct	1012:1017	arg1	analysis					1026:1033	direct glycan analysis	1012:1033	direct glycan analysis	1012:1033	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	7	5	theme	predicted	1540:1548	arg1	functions					1550:1558	the predicted functions	1536:1558	the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1536:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	2	6	theme	type	469:472	arg1	roles					408:412	biological roles	397:412	biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins	397:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	5	7	theme	efficient	1098:1106	arg1	constructs					1123:1132	efficient gene targeting constructs	1098:1132	efficient gene targeting constructs	1098:1132	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	4	8	theme	facile	829:834	arg1	tool					861:864	the facile CRISPR/Cas9 gene editing tool	825:864	the facile CRISPR/Cas9 gene editing tool	825:864	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	2	9	gly	glycosites	532:541	arg1	glycoconjugate					477:490	glycoconjugate	477:490	glycoconjugate	477:490	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	9	gly	glycosites	532:541	arg2	glycosites					532:541	distinct glycosites	523:541	distinct glycosites	523:541	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	9	gly	glycosites	532:541	arg2	glycoconjugate					477:490	glycoconjugate	477:490	glycoconjugate	477:490	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	5	10	theme	gene	1224:1227	arg1	function					1229:1236	gene function	1224:1236	gene function	1224:1236	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	7	11	theme	functions	1550:1558	arg1	view					1528:1531	a global view	1519:1531	a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1519:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	5	12	theme	gene	1108:1111	arg1	constructs					1123:1132	efficient gene targeting constructs	1098:1132	efficient gene targeting constructs	1098:1132	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	2	13	theme	analytic	336:343	arg1	strategies					345:354	analytic strategies	336:354	analytic strategies	336:354	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	7	14	theme	gRNA	1400:1403	arg1	designs					1405:1411	validated high-efficiency gRNA designs	1374:1411	validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1374:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	5	15	theme	function	1229:1236	arg1	targeting					1191:1199	gene targeting	1186:1199	gene targeting	1186:1199	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	15	theme	function	1229:1236	arg1	translation					1209:1219	the translation	1205:1219	the translation of gene function to glycan structures	1205:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	1	16	theme	different	251:259	arg1	glycoconjugates					261:275	different glycoconjugates	251:275	different glycoconjugates in cells	251:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	6	17	theme	gene	1329:1332	arg1	families					1334:1341	isoenzyme gene families	1319:1341	isoenzyme gene families	1319:1341	This is especially true for glycosylation steps covered by isoenzyme gene families.
29315387	4	18	theme	gene	848:851	arg1	tool					861:864	the facile CRISPR/Cas9 gene editing tool	825:864	the facile CRISPR/Cas9 gene editing tool	825:864	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	7	19	theme	high-efficiency	1384:1398	arg1	designs					1405:1411	validated high-efficiency gRNA designs	1374:1411	validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1374:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	2	20	theme	glycoproteins	504:516	arg1	roles					408:412	biological roles	397:412	biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins	397:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	3	21	theme	most	712:715	arg1	roles					703:707	the biosynthetic roles	686:707	the biosynthetic roles of most of these enzymes	686:732	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	2	22	from	advancements	320:331	arg1	strategies					345:354	analytic strategies	336:354	analytic strategies	336:354	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	5	23	from	translation	1209:1219	arg1	results					1173:1179	results	1173:1179	results from gene targeting and the translation of gene function to glycan structures	1173:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	23	from	translation	1209:1219	arg1	interpretation					1155:1168	the interpretation	1151:1168	the interpretation of results from gene targeting and the translation of gene function to glycan structures	1151:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	3	24	dep	this	571:574	arg1	contrast					559:566	contrast	559:566	contrast	559:566	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	2	25	theme	particular	493:502	arg1	glycoproteins					504:516	particular glycoproteins	493:516	particular glycoproteins	493:516	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	3	26	theme	biosynthetic	690:701	arg1	roles					703:707	the biosynthetic roles	686:707	the biosynthetic roles of most of these enzymes	686:732	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	2	27	gly	glycoproteins	504:516	arg1	glycoproteins					504:516	particular glycoproteins	493:516	particular glycoproteins	493:516	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	6	28	theme	isoenzyme	1319:1327	arg1	families					1334:1341	isoenzyme gene families	1319:1341	isoenzyme gene families	1319:1341	This is especially true for glycosylation steps covered by isoenzyme gene families.
29315387	0	29	theme	glycosyltransferase	64:82	arg1	genome					84:89	the human glycosyltransferase genome	54:89	the human glycosyltransferase genome	54:89	A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome.
29315387	4	30	theme	glycome	936:942	arg1	functions					919:927	the functions	915:927	the functions of the glycome	915:942	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	1	31	theme	human	183:187	arg1	glycome					189:195	the human glycome	179:195	the human glycome	179:195	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	5	32	from	interpretation	1155:1168	arg1	targeting					1191:1199	gene targeting	1186:1199	gene targeting	1186:1199	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	32	from	interpretation	1155:1168	arg1	translation					1209:1219	the translation	1205:1219	the translation of gene function to glycan structures	1205:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	7	33	theme	glycosyltransferase	1478:1496	arg1	genome					1498:1503	the human glycosyltransferase genome	1468:1503	the human glycosyltransferase genome	1468:1503	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	4	34	theme	pathways	987:994	arg1	dissection					960:969	genetic dissection	952:969	genetic dissection of biosynthetic pathways	952:994	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	7	35	theme	genome	1498:1503	arg1	individual					1426:1435	individual	1426:1435	individual	1426:1435	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	7	35	theme	genome	1498:1503	arg1	targeting					1455:1463	combinatorial targeting	1441:1463	combinatorial targeting	1441:1463	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	7	36	theme	human	1658:1662	arg1	glycome					1664:1670	the human glycome	1654:1670	the human glycome	1654:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	1	37	theme	glycome	189:195	arg1	biosynthesis					163:174	the biosynthesis	159:174	the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells	159:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	7	38	theme	glycosyltransferases	1569:1588	arg1	functions					1550:1558	the predicted functions	1536:1558	the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1536:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	2	39	theme	glycans	417:423	arg1	roles					408:412	biological roles	397:412	biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins	397:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	7	40	theme	targeting	1619:1627	arg1	strategies					1629:1638	gene targeting strategies	1614:1638	gene targeting strategies	1614:1638	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	1	41	from	glycoconjugates	261:275	arg1	cells					280:284	cells	280:284	cells	280:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	7	42	theme	global	1521:1526	arg1	view					1528:1531	a global view	1519:1531	a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1519:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	1	43	theme	biosynthesis	163:174	arg1	orchestration					142:154	the orchestration	138:154	the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells	138:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	0	44	theme	CRISPR/Cas9	29:39	arg1	targeting					41:49	CRISPR/Cas9 targeting	29:49	CRISPR/Cas9 targeting of the human glycosyltransferase genome	29:89	A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome.
29315387	2	45	theme	glycosites	532:541	arg1	roles					408:412	biological roles	397:412	biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins	397:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	5	46	with	validation	1084:1093	arg1	interpretation					1155:1168	the interpretation	1151:1168	the interpretation of results from gene targeting and the translation of gene function to glycan structures	1151:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	7	47	theme	designs	1405:1411	arg1	library					1363:1369	a library	1361:1369	a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1361:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	2	48	theme	biological	397:406	arg1	roles					408:412	biological roles	397:412	biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins	397:553	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	4	49	theme	biosynthetic	974:985	arg1	pathways					987:994	biosynthetic pathways	974:994	biosynthetic pathways	974:994	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	2	50	theme	distinct	523:530	arg1	glycoconjugate					477:490	glycoconjugate	477:490	glycoconjugate	477:490	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	50	theme	distinct	523:530	arg1	glycosites					532:541	distinct glycosites	523:541	distinct glycosites	523:541	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	6	51	theme	glycosylation	1288:1300	arg1	steps					1302:1306	glycosylation steps	1288:1306	glycosylation steps covered by isoenzyme gene families	1288:1341	This is especially true for glycosylation steps covered by isoenzyme gene families.
29315387	7	52	theme	combinatorial	1441:1453	arg1	targeting					1455:1463	combinatorial targeting	1441:1463	combinatorial targeting	1441:1463	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	4	53	theme	functions	919:927	arg1	investigation					898:910	investigation	898:910	investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis	898:1033	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	2	54	theme	glycan	450:455	arg1	structures					457:466	specific glycan structures	441:466	specific glycan structures	441:466	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	5	55	theme	targeting	1113:1121	arg1	constructs					1123:1132	efficient gene targeting constructs	1098:1132	efficient gene targeting constructs	1098:1132	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	7	56	theme	suitable	1413:1420	arg1	designs					1405:1411	validated high-efficiency gRNA designs	1374:1411	validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1374:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	2	57	theme	specific	441:448	arg1	structures					457:466	specific glycan structures	441:466	specific glycan structures	441:466	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	4	58	theme	tool	861:864	arg1	availability					809:820	the availability	805:820	the availability of the facile CRISPR/Cas9 gene editing tool	805:864	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	3	59	theme	glycome	659:665	arg1	biosynthesis					633:644	the biosynthesis	629:644	the biosynthesis of the human glycome	629:665	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	4	60	theme	editing	853:859	arg1	tool					861:864	the facile CRISPR/Cas9 gene editing tool	825:864	the facile CRISPR/Cas9 gene editing tool	825:864	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	5	61	with	design	1073:1078	arg1	interpretation					1155:1168	the interpretation	1151:1168	the interpretation of results from gene targeting and the translation of gene function to glycan structures	1151:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	3	62	theme	glycosyltransferase	591:609	arg1	genes					611:615	glycosyltransferase genes	591:615	glycosyltransferase genes involved in the biosynthesis of the human glycome	591:665	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	3	63	theme	genes	611:615	arg1	manageable					670:679	manageable	670:679	manageable	670:679	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	3	63	theme	genes	611:615	arg1	number					581:586	the number	577:586	the number of glycosyltransferase genes involved in the biosynthesis of the human glycome	577:665	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	7	64	theme	validated	1374:1382	arg1	designs					1405:1411	validated high-efficiency gRNA designs	1374:1411	validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome	1374:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	5	65	theme	glycan	1241:1246	arg1	structures					1248:1257	glycan structures	1241:1257	glycan structures	1241:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	3	66	theme	reasonable	771:780	arg1	confidence					782:791	reasonable confidence	771:791	reasonable confidence	771:791	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	5	67	from	targeting	1191:1199	arg1	results					1173:1179	results	1173:1179	results from gene targeting and the translation of gene function to glycan structures	1173:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	67	from	targeting	1191:1199	arg1	interpretation					1155:1168	the interpretation	1151:1168	the interpretation of results from gene targeting and the translation of gene function to glycan structures	1151:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	7	68	theme	human	1472:1476	arg1	genome					1498:1503	the human glycosyltransferase genome	1468:1503	the human glycosyltransferase genome	1468:1503	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	1	69	located	found	242:246	arg2	structures					231:240	all glycan structures	220:240	all glycan structures found on different glycoconjugates in cells	220:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	1	69	located	found	242:246	arg1	glycoconjugates					261:275	different glycoconjugates	251:275	different glycoconjugates in cells	251:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	7	70	theme	gene	1614:1617	arg1	strategies					1629:1638	gene targeting strategies	1614:1638	gene targeting strategies	1614:1638	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	7	71	theme	glycome	1664:1670	arg1	studies					1643:1649	studies	1643:1649	studies of the human glycome	1643:1670	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	0	72	theme	human	58:62	arg1	genome					84:89	the human glycosyltransferase genome	54:89	the human glycosyltransferase genome	54:89	A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome.
29315387	3	73	theme	human	653:657	arg1	glycome					659:665	the human glycome	649:665	the human glycome	649:665	In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence.
29315387	7	74	theme	human	1563:1567	arg1	glycosyltransferases					1569:1588	human glycosyltransferases	1563:1588	human glycosyltransferases	1563:1588	Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.
29315387	1	75	theme	glycan	224:229	arg1	structures					231:240	all glycan structures	220:240	all glycan structures found on different glycoconjugates in cells	220:284	Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in cells.
29315387	2	76	theme	glycoconjugate	477:490	arg1	glycoconjugate					477:490	glycoconjugate	477:490	glycoconjugate	477:490	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	76	theme	glycoconjugate	477:490	arg1	glycoproteins					504:516	particular glycoproteins	493:516	particular glycoproteins	493:516	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	76	theme	glycoconjugate	477:490	arg1	glycans					417:423	glycans	417:423	glycans with respect to specific glycan structures	417:466	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	76	theme	glycoconjugate	477:490	arg1	type					469:472	type	469:472	type of glycoconjugate	469:490	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	2	76	theme	glycoconjugate	477:490	arg1	glycosites					532:541	distinct glycosites	523:541	distinct glycosites	523:541	The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins.
29315387	0	77	theme	gRNA	12:15	arg1	library					17:23	gRNA library	12:23	gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome	12:89	A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome.
29315387	5	78	theme	results	1173:1179	arg1	interpretation					1155:1168	the interpretation	1151:1168	the interpretation of results from gene targeting and the translation of gene function to glycan structures	1151:1257	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	79	theme	gene	1186:1189	arg1	targeting					1191:1199	gene targeting	1186:1199	gene targeting	1186:1199	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	4	80	theme	glycan	1019:1024	arg1	analysis					1026:1033	direct glycan analysis	1012:1033	direct glycan analysis	1012:1033	Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis.
29315387	5	81	theme	constructs	1123:1132	arg1	design					1073:1078	design	1073:1078	design	1073:1078	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
29315387	5	81	theme	constructs	1123:1132	arg1	validation					1084:1093	validation	1084:1093	validation	1084:1093	However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures.
31891675	4	0	dep	length	657:662	arg1	degree					686:691	degree	686:691	degree of deacetylation, degree of sulfation	686:729	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	1	theme	transfection	782:793	arg1	efficiency					795:804	transfection efficiency	782:804	transfection efficiency	782:804	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	2	theme	avidity	828:834	arg1	mRNA					839:842	avidity to mRNA	828:842	avidity to mRNA	828:842	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	5	3	theme	%	992:992	arg1	efficiency					1007:1016	∼60%-65% transfection efficiency	985:1016	∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	985:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	0	4	theme	Composition	85:95	arg1	Influence					72:80	Influence	72:80	Influence of Composition and Structure	72:109	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	1	5	dep	acids	306:310	arg1	HAs					313:315	HAs	313:315	HAs	313:315	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	1	5	dep	acids	306:310	arg1	native					319:324	native	319:324	native	319:324	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	1	5	dep	acids	306:310	arg1	sulfated					330:337	sulfated	330:337	sulfated	330:337	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	1	6	theme	deacetylation	277:289	arg1	degrees					266:272	different degrees	256:272	different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	256:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	5	7	theme	lipid	1053:1057	arg1	control					1059:1065	commercially available lipid control	1030:1065	commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	1030:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	3	8	theme	pH.	569:571	arg1	size					582:585	pH. Particle size	569:585	pH. Particle size	569:585	In vitro transfections were performed in the presence/absence of trehalose and at different pH. Particle size and ζ-potential were correlated with transfection efficiency.
31891675	4	9	theme	deacetylation	696:708	arg1	degree					686:691	degree	686:691	degree of deacetylation, degree of sulfation	686:729	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	1	10	theme	RNA	122:124	arg1	nanoparticles					144:156	Messenger RNA (mRNA)-containing nanoparticles	112:156	Messenger RNA (mRNA)-containing nanoparticles	112:156	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	0	11	theme	Structure	101:109	arg1	Influence					72:80	Influence	72:80	Influence of Composition and Structure	72:109	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	4	12	from	influence	769:777	arg1	efficiency					795:804	transfection efficiency	782:804	transfection efficiency	782:804	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	5	13	with	control	1059:1065	arg1	toxicity					1084:1091	no apparent toxicity	1072:1091	no apparent toxicity for transfection at slightly acidic pH 6.5	1072:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	14	theme	hyaluronic	295:304	arg1	acids					306:310	hyaluronic acids	295:310	hyaluronic acids (HAs) (native vs. sulfated)	295:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	5	15	theme	transfection	994:1005	arg1	efficiency					1007:1016	∼60%-65% transfection efficiency	985:1016	∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	985:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	16	theme	electrostatic	175:187	arg1	complexation					189:200	electrostatic complexation	175:200	electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	175:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	4	17	dep	mRNA	839:842	arg1	to					836:837	to	836:837	to	836:837	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	5	18	theme	P	847:847	arg1	ratio					851:855	P:C ratio	847:855	P:C ratio	847:855	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	5	19	theme	relative	1018:1025	arg1	efficiency					1007:1016	∼60%-65% transfection efficiency	985:1016	∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	985:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	2	20	dep	phosphate	404:412	arg1	to					401:402	to	401:402	to	401:402	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	3	21	theme	In vitro	477:484	arg1	transfections					486:498	In vitro transfections	477:498	In vitro transfections	477:498	In vitro transfections were performed in the presence/absence of trehalose and at different pH. Particle size and ζ-potential were correlated with transfection efficiency.
31891675	5	22	theme	available	1043:1051	arg1	control					1059:1065	commercially available lipid control	1030:1065	commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	1030:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	23	theme	acids	306:310	arg1	degrees					266:272	different degrees	256:272	different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	256:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	4	24	theme	direct	762:767	arg1	influence					769:777	a direct influence	760:777	a direct influence on transfection efficiency	760:804	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	25	theme	charge	668:673	arg1	densities					675:683	Polymer length and charge densities	649:683	densities	675:683	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	2	26	theme	HA	362:363	arg1	degree					365:370	HA degree	362:370	HA degree of sulfation (DS)	362:388	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	0	27	theme	mRNA	40:43	arg1	Systems					54:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	2	28	dep	N	453:453	arg1	C					457:457	C	457:457	N:P:C	453:457	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	28	dep	N	453:453	arg1	P					455:455	P	455:455	N:P:C	453:457	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	1	29	theme	Messenger	112:120	arg1	mRNA					127:130	mRNA	127:130	mRNA	127:130	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	1	29	theme	Messenger	112:120	arg1	RNA					122:124	Messenger RNA	112:124	Messenger RNA (mRNA)-containing nanoparticles	112:156	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	5	30	theme	acid	903:906	arg1	dose					908:911	nucleic acid dose	895:911	nucleic acid dose	895:911	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	0	31	theme	Chitosan/Hyaluronan-Based	14:38	arg1	Systems					54:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	4	32	theme	mRNA	839:842	arg1	modulation					814:823	modulation	814:823	modulation of avidity to mRNA	814:842	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	2	33	from	HA	442:443	arg1	sulfate					428:434	carboxyl + sulfate	417:434	carboxyl + sulfate (from HA) ratio (N:P:C)	417:458	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	0	34	dep	Influence	72:80	arg1	Efficiency					0:9	Efficiency	0:9	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems	0:60	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	0	34	dep	Influence	72:80	arg1	In Vitro					62:69	In Vitro	62:69	In Vitro	62:69	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	0	35	theme	Systems	54:60	arg1	Efficiency					0:9	Efficiency	0:9	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems	0:60	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	3	36	theme	Particle	573:580	arg1	size					582:585	pH. Particle size	569:585	pH. Particle size	569:585	In vitro transfections were performed in the presence/absence of trehalose and at different pH. Particle size and ζ-potential were correlated with transfection efficiency.
31891675	4	37	theme	HA	740:741	arg1	length					657:662	Polymer length and charge densities	649:683	length	657:662	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	37	theme	HA	740:741	arg1	densities					675:683	Polymer length and charge densities	649:683	densities	675:683	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	1	38	theme	pharmaceutical	220:233	arg1	chitosans					241:249	pharmaceutical grade chitosans	220:249	pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	220:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	0	39	theme	Delivery	45:52	arg1	Systems					54:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Chitosan/Hyaluronan-Based mRNA Delivery Systems	14:60	Efficiency of Chitosan/Hyaluronan-Based mRNA Delivery Systems In Vitro: Influence of Composition and Structure.
31891675	2	40	theme	sulfate	428:434	arg1	ratio					446:450	carboxyl + sulfate (from HA) ratio	417:450	carboxyl + sulfate (from HA) ratio (N:P:C)	417:458	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	41	theme	amine	395:399	arg1	phosphate					404:412	amine to phosphate	395:412	amine to phosphate	395:412	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	1	42	theme	grade	235:239	arg1	chitosans					241:249	pharmaceutical grade chitosans	220:249	pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	220:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	2	43	theme	carboxyl +	417:426	arg1	sulfate					428:434	carboxyl + sulfate	417:434	carboxyl + sulfate (from HA) ratio (N:P:C)	417:458	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	5	44	theme	-65	989:991	arg1	%					988:988	%	988:988	%	988:988	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	4	45	theme	chitosan	747:754	arg1	length					657:662	Polymer length and charge densities	649:683	length	657:662	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	45	theme	chitosan	747:754	arg1	densities					675:683	Polymer length and charge densities	649:683	densities	675:683	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	46	theme	Polymer	649:655	arg1	length					657:662	Polymer length and charge densities	649:683	length	657:662	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	2	47	theme	sulfation	375:383	arg1	phosphate					404:412	amine to phosphate	395:412	amine to phosphate	395:412	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	47	theme	sulfation	375:383	arg1	Mn					357:358	Mn	357:358	Mn	357:358	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	47	theme	sulfation	375:383	arg1	length					349:354	Polymer length	341:354	Polymer length (Mn)	341:359	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	47	theme	sulfation	375:383	arg1	degree					365:370	HA degree	362:370	HA degree of sulfation (DS)	362:388	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	5	48	from	pH	1129:1130	arg1	transfection					1097:1108	transfection	1097:1108	transfection at slightly acidic pH 6.5	1097:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	5	49	theme	transfection	924:935	arg1	efficiency					937:946	transfection efficiency	924:946	transfection efficiency	924:946	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	2	50	theme	Polymer	341:347	arg1	Mn					357:358	Mn	357:358	Mn	357:358	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	2	50	theme	Polymer	341:347	arg1	length					349:354	Polymer length	341:354	Polymer length (Mn)	341:359	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	5	51	theme	apparent	1075:1082	arg1	toxicity					1084:1091	no apparent toxicity	1072:1091	no apparent toxicity for transfection at slightly acidic pH 6.5	1072:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	2	52	dep	ratio	446:450	arg1	N					453:453	N	453:453	N:P:C	453:457	Polymer length (Mn), HA degree of sulfation (DS), and amine to phosphate to carboxyl + sulfate (from HA) ratio (N:P:C) were controlled.
31891675	4	53	theme	sulfation	721:729	arg1	deacetylation					696:708	deacetylation	696:708	deacetylation	696:708	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	53	theme	sulfation	721:729	arg1	degree					711:716	degree	711:716	degree of sulfation	711:729	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	3	54	theme	trehalose	542:550	arg1	presence/absence					522:537	the presence/absence	518:537	the presence/absence of trehalose	518:550	In vitro transfections were performed in the presence/absence of trehalose and at different pH. Particle size and ζ-potential were correlated with transfection efficiency.
31891675	5	55	theme	C	849:849	arg1	ratio					851:855	P:C ratio	847:855	P:C ratio	847:855	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	56	theme	chitosans	241:249	arg1	library					209:215	a library	207:215	a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	207:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	1	57	with	complexation	189:200	arg1	library					209:215	a library	207:215	a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	207:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	5	58	theme	optimized	953:961	arg1	formulations					963:974	optimized formulations	953:974	optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5	953:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	5	59	theme	∼60	985:987	arg1	%					988:988	%	988:988	%	988:988	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	60	theme	-containing	132:142	arg1	nanoparticles					144:156	Messenger RNA (mRNA)-containing nanoparticles	112:156	Messenger RNA (mRNA)-containing nanoparticles	112:156	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	4	61	contain	had	756:758	arg2	influence					769:777	a direct influence	760:777	a direct influence on transfection efficiency	760:804	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	61	contain	had	756:758	arg1	length					657:662	Polymer length and charge densities	649:683	length	657:662	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	4	61	contain	had	756:758	arg1	densities					675:683	Polymer length and charge densities	649:683	densities	675:683	Polymer length and charge densities (degree of deacetylation, degree of sulfation) of both HA and chitosan had a direct influence on transfection efficiency through modulation of avidity to mRNA.
31891675	1	62	theme	different	256:264	arg1	degrees					266:272	different degrees	256:272	different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	256:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	3	63	theme	transfection	624:635	arg1	efficiency					637:646	transfection efficiency	624:646	transfection efficiency	624:646	In vitro transfections were performed in the presence/absence of trehalose and at different pH. Particle size and ζ-potential were correlated with transfection efficiency.
31891675	5	64	theme	nucleic	895:901	arg1	dose					908:911	nucleic acid dose	895:911	nucleic acid dose	895:911	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	1	65	with	chitosans	241:249	arg1	degrees					266:272	different degrees	256:272	different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated)	256:338	Messenger RNA (mRNA)-containing nanoparticles were produced by electrostatic complexation with a library of pharmaceutical grade chitosans with different degrees of deacetylation and hyaluronic acids (HAs) (native vs. sulfated).
31891675	5	66	theme	mixing	869:874	arg1	concentration					876:888	mixing concentration	869:888	mixing concentration	869:888	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31891675	5	67	theme	acidic	1122:1127	arg1	pH					1129:1130	slightly acidic pH 6.5	1113:1134	slightly acidic pH 6.5	1113:1134	N:P:C ratio, trehalose, mixing concentration, and nucleic acid dose influenced transfection efficiency with optimized formulations reaching ∼60%-65% transfection efficiency relative to commercially available lipid control with no apparent toxicity for transfection at slightly acidic pH 6.5.
31047665	0	0	theme	enhanced	96:103	arg1	performance					123:133	enhanced enantioseparation performance	96:133	enhanced enantioseparation performance	96:133	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	1	1	theme	GQDs	336:339	arg1	effect					326:331	the effect	322:331	the effect of GQDs on chiral separation	322:360	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	2	2	dep	found	503:507	arg1	enhanced					514:521	enhanced	514:521	found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative	503:668	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	4	3	theme	recognition	1002:1012	arg1	ability					1014:1020	the chiral recognition ability	991:1020	the chiral recognition ability	991:1020	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	1	4	from	effect	326:331	arg1	separation					351:360	chiral separation	344:360	chiral separation	344:360	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	5	5	theme	experimental	1100:1111	arg1	results					1113:1119	the experimental results	1096:1119	the experimental results	1096:1119	The molecular simulation results showed a good agreement with the experimental results.
31047665	1	6	theme	chiral	344:349	arg1	separation					351:360	chiral separation	344:360	chiral separation	344:360	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	6	7	theme	asymmetric	1342:1351	arg1	synthesis					1353:1361	asymmetric synthesis	1342:1361	asymmetric synthesis	1342:1361	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	5	8	theme	good	1076:1079	arg1	agreement					1081:1089	a good agreement	1074:1089	a good agreement with the experimental results	1074:1119	The molecular simulation results showed a good agreement with the experimental results.
31047665	1	9	theme	chiral	276:281	arg1	CSP					302:304	CSP	302:304	CSP	302:304	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	9	theme	chiral	276:281	arg1	phases					294:299	chiral stationary phases	276:299	chiral stationary phases (CSP)	276:305	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	9	theme	chiral	276:281	arg1	dots					153:156	Graphene quantum dots	136:156	Graphene quantum dots (GQD)	136:162	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	0	10	theme	enantioseparation	105:121	arg1	performance					123:133	enhanced enantioseparation performance	96:133	enhanced enantioseparation performance	96:133	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	1	11	theme	stationary	283:292	arg1	CSP					302:304	CSP	302:304	CSP	302:304	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	11	theme	stationary	283:292	arg1	phases					294:299	chiral stationary phases	276:299	chiral stationary phases (CSP)	276:305	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	11	theme	stationary	283:292	arg1	dots					153:156	Graphene quantum dots	136:156	Graphene quantum dots (GQD)	136:162	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	4	12	theme	molecular	810:818	arg1	results					831:837	molecular simulation results	810:837	molecular simulation results	810:837	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	6	13	theme	chiral	1382:1387	arg1	recognition					1389:1399	chiral recognition	1382:1399	chiral recognition	1382:1399	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	14	theme	chiral	1290:1295	arg1	separation					1297:1306	chiral separation	1290:1306	chiral separation	1290:1306	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	1	15	dep	dots	153:156	arg1	β-CD					195:198	β-CD	195:198	β-CD	195:198	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	15	dep	dots	153:156	arg1	β-cyclodextrin					179:192	functionalized β-cyclodextrin	164:192	functionalized β-cyclodextrin (β-CD)	164:199	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	0	16	theme	quantum	9:15	arg1	dots					17:20	Graphene quantum dots	0:20	Graphene quantum dots	0:20	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	2	17	theme	enantioseparation	385:401	arg1	performance					403:413	the enantioseparation performance	381:413	the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs	381:459	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	0	18	theme	Graphene	0:7	arg1	dots					17:20	Graphene quantum dots	0:20	Graphene quantum dots	0:20	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	6	19	theme	chiral	1364:1369	arg1	catalysis					1371:1379	chiral catalysis	1364:1379	chiral catalysis	1364:1379	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	20	theme	great	1272:1276	arg1	prospect					1278:1285	great prospect	1272:1285	great prospect	1272:1285	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	1	21	theme	cellulose	205:213	arg1	composites					222:231	cellulose silica composites	205:231	cellulose silica composites	205:231	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	2	22	theme	CSPs	456:459	arg1	performance					403:413	the enantioseparation performance	381:413	the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs	381:459	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	22	theme	CSPs	456:459	arg1	β-CD					476:479	unmodified β-CD	465:479	unmodified β-CD	465:479	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	22	theme	CSPs	456:459	arg1	CSPs					494:497	cellulose CSPs	484:497	cellulose CSPs	484:497	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	5	23	theme	simulation	1048:1057	arg1	results					1059:1065	The molecular simulation results	1034:1065	The molecular simulation results	1034:1065	The molecular simulation results showed a good agreement with the experimental results.
31047665	4	24	theme	extra	853:857	arg1	interactions					859:870	extra interactions	853:870	extra interactions such as hydrophobic	853:890	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	4	24	theme	extra	853:857	arg1	hydrophobic					880:890	hydrophobic	880:890	hydrophobic	880:890	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	1	25	theme	silica	215:220	arg1	composites					222:231	cellulose silica composites	205:231	cellulose silica composites	205:231	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	2	26	theme	cellulose	446:454	arg1	CSPs					456:459	cellulose CSPs	446:459	cellulose CSPs	446:459	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	1	27	theme	Graphene	136:143	arg1	phases					294:299	chiral stationary phases	276:299	chiral stationary phases (CSP)	276:305	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	27	theme	Graphene	136:143	arg1	GQD					159:161	GQD	159:161	GQD	159:161	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	27	theme	Graphene	136:143	arg1	dots					153:156	Graphene quantum dots	136:156	Graphene quantum dots (GQD)	136:162	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	2	28	theme	β-CD	438:441	arg1	performance					403:413	the enantioseparation performance	381:413	the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs	381:459	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	28	theme	β-CD	438:441	arg1	β-CD					476:479	unmodified β-CD	465:479	unmodified β-CD	465:479	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	28	theme	β-CD	438:441	arg1	CSPs					494:497	cellulose CSPs	484:497	cellulose CSPs	484:497	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	5	29	with	agreement	1081:1089	arg1	results					1113:1119	the experimental results	1096:1119	the experimental results	1096:1119	The molecular simulation results showed a good agreement with the experimental results.
31047665	2	30	theme	unmodified	465:474	arg1	β-CD					476:479	unmodified β-CD	465:479	unmodified β-CD	465:479	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	31	theme	nature	560:565	arg1	performance					545:555	the enantioseparation performance	523:555	the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative	523:668	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	6	32	theme	GQDs	1170:1173	arg1	performance					1155:1165	the enhancement performance	1139:1165	the enhancement performance of GQDs for chiral separation	1139:1195	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	3	33	theme	Molecular	671:679	arg1	modeling					681:688	Molecular modeling	671:688	Molecular modeling	671:688	Molecular modeling was applied to understand and theoretically study the enhancement mechanism of GQDs for enantioseparation.
31047665	2	34	theme	functionalized	423:436	arg1	β-CD					438:441	GQDs functionalized β-CD	418:441	GQDs functionalized β-CD	418:441	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	0	35	theme	β-cyclodextrin	37:50	arg1	phases					84:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	6	36	theme	drug	1405:1408	arg1	delivery					1410:1417	drug delivery	1405:1417	drug delivery	1405:1417	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	2	37	theme	cellulose	484:492	arg1	CSPs					494:497	cellulose CSPs	484:497	cellulose CSPs	484:497	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	4	38	theme	π-π	911:913	arg1	interaction					915:925	π-π interaction	911:925	π-π interaction	911:925	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	2	39	theme	GQDs	418:421	arg1	β-CD					438:441	GQDs functionalized β-CD	418:441	GQDs functionalized β-CD	418:441	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	0	40	theme	chiral	66:71	arg1	phases					84:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	2	41	theme	cellulose-3,5-dimethylphenylcarbamate	621:657	arg1	derivative					659:668	cellulose-3,5-dimethylphenylcarbamate derivative	621:668	cellulose-3,5-dimethylphenylcarbamate derivative	621:668	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	41	theme	cellulose-3,5-dimethylphenylcarbamate	621:657	arg1	nature					560:565	nature β-CD	560:570	nature β-CD	560:570	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	4	42	theme	chiral	932:937	arg1	selector					939:946	chiral selector	932:946	chiral selector	932:946	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	1	43	theme	quantum	145:151	arg1	phases					294:299	chiral stationary phases	276:299	chiral stationary phases (CSP)	276:305	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	43	theme	quantum	145:151	arg1	GQD					159:161	GQD	159:161	GQD	159:161	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	43	theme	quantum	145:151	arg1	dots					153:156	Graphene quantum dots	136:156	Graphene quantum dots (GQD)	136:162	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	0	44	theme	cellulose	56:64	arg1	phases					84:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	4	45	theme	chiral	995:1000	arg1	ability					1014:1020	the chiral recognition ability	991:1020	the chiral recognition ability	991:1020	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	6	46	contain	have	1267:1270	arg1	composites					1240:1249	GQDs-based chiral composites	1222:1249	GQDs-based chiral composites	1222:1249	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	46	contain	have	1267:1270	arg1	GQDs					1262:1265	chiral GQDs	1255:1265	chiral GQDs	1255:1265	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	46	contain	have	1267:1270	arg2	prospect					1278:1285	great prospect	1272:1285	great prospect	1272:1285	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	3	47	theme	GQDs	769:772	arg1	mechanism					756:764	the enhancement mechanism	740:764	the enhancement mechanism of GQDs for enantioseparation	740:794	Molecular modeling was applied to understand and theoretically study the enhancement mechanism of GQDs for enantioseparation.
31047665	4	48	theme	hydrogen	893:900	arg1	bond					902:905	hydrogen bond	893:905	hydrogen bond	893:905	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	5	49	theme	molecular	1038:1046	arg1	results					1059:1065	The molecular simulation results	1034:1065	The molecular simulation results	1034:1065	The molecular simulation results showed a good agreement with the experimental results.
31047665	6	50	theme	chiral	1233:1238	arg1	composites					1240:1249	GQDs-based chiral composites	1222:1249	GQDs-based chiral composites	1222:1249	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	2	51	theme	β-CD-3,5-dimethylphenylcarbamate	573:604	arg1	derivative					606:615	β-CD-3,5-dimethylphenylcarbamate derivative	573:615	β-CD-3,5-dimethylphenylcarbamate derivative	573:615	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	2	51	theme	β-CD-3,5-dimethylphenylcarbamate	573:604	arg1	nature					560:565	nature β-CD	560:570	nature β-CD	560:570	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
31047665	3	52	theme	enhancement	744:754	arg1	mechanism					756:764	the enhancement mechanism	740:764	the enhancement mechanism of GQDs for enantioseparation	740:794	Molecular modeling was applied to understand and theoretically study the enhancement mechanism of GQDs for enantioseparation.
31047665	4	53	theme	simulation	820:829	arg1	results					831:837	molecular simulation results	810:837	molecular simulation results	810:837	According to molecular simulation results, GQDs provide extra interactions such as hydrophobic, hydrogen bond and π-π interaction when chiral selector interacts with enantiomers, which enhances the chiral recognition ability indirectly.
31047665	6	54	theme	chiral	1255:1260	arg1	GQDs					1262:1265	chiral GQDs	1255:1265	chiral GQDs	1255:1265	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	55	theme	enhancement	1143:1153	arg1	performance					1155:1165	the enhancement performance	1139:1165	the enhancement performance of GQDs for chiral separation	1139:1195	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	56	theme	research	1318:1325	arg1	fields					1327:1332	other research fields	1312:1332	other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery	1312:1417	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	56	theme	research	1318:1325	arg1	synthesis					1353:1361	asymmetric synthesis	1342:1361	asymmetric synthesis	1342:1361	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	56	theme	research	1318:1325	arg1	catalysis					1371:1379	chiral catalysis	1364:1379	chiral catalysis	1364:1379	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	56	theme	research	1318:1325	arg1	recognition					1389:1399	chiral recognition	1382:1399	chiral recognition	1382:1399	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	56	theme	research	1318:1325	arg1	delivery					1410:1417	drug delivery	1405:1417	drug delivery	1405:1417	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	57	theme	chiral	1179:1184	arg1	separation					1186:1195	chiral separation	1179:1195	chiral separation	1179:1195	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	58	theme	GQDs-based	1222:1231	arg1	composites					1240:1249	GQDs-based chiral composites	1222:1249	GQDs-based chiral composites	1222:1249	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	0	59	theme	stationary	73:82	arg1	phases					84:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	β-cyclodextrin and cellulose chiral stationary phases	37:89	Graphene quantum dots functionalized β-cyclodextrin and cellulose chiral stationary phases with enhanced enantioseparation performance.
31047665	6	60	theme	other	1312:1316	arg1	fields					1327:1332	other research fields	1312:1332	other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery	1312:1417	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	60	theme	other	1312:1316	arg1	synthesis					1353:1361	asymmetric synthesis	1342:1361	asymmetric synthesis	1342:1361	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	60	theme	other	1312:1316	arg1	catalysis					1371:1379	chiral catalysis	1364:1379	chiral catalysis	1364:1379	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	60	theme	other	1312:1316	arg1	recognition					1389:1399	chiral recognition	1382:1399	chiral recognition	1382:1399	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	6	60	theme	other	1312:1316	arg1	delivery					1410:1417	drug delivery	1405:1417	drug delivery	1405:1417	Our work reveals the enhancement performance of GQDs for chiral separation, it can be expected that GQDs-based chiral composites and chiral GQDs have great prospect in chiral separation and other research fields such as asymmetric synthesis, chiral catalysis, chiral recognition and drug delivery.
31047665	1	61	theme	functionalized	164:177	arg1	β-CD					195:198	β-CD	195:198	β-CD	195:198	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	1	61	theme	functionalized	164:177	arg1	β-cyclodextrin					179:192	functionalized β-cyclodextrin	164:192	functionalized β-cyclodextrin (β-CD)	164:199	Graphene quantum dots (GQD) functionalized β-cyclodextrin (β-CD) and cellulose silica composites were first prepared and applied in HPLC as chiral stationary phases (CSP) to investigate the effect of GQDs on chiral separation.
31047665	2	62	theme	enantioseparation	527:543	arg1	performance					545:555	the enantioseparation performance	523:555	the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative	523:668	Through comparing the enantioseparation performance of GQDs functionalized β-CD or cellulose CSPs and unmodified β-CD or cellulose CSPs, we found GQDs enhanced the enantioseparation performance of nature β-CD, β-CD-3,5-dimethylphenylcarbamate derivative and cellulose-3,5-dimethylphenylcarbamate derivative.
30933879	6	0	theme	amino	854:858	arg1	acids					860:864	amino acids	854:864	amino acids	854:864	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	6	1	located	detected	789:796	arg2	metabolites					772:782	63 and 67 hydrophilic metabolites	750:782	63 and 67 hydrophilic metabolites	750:782	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	6	1	located	detected	789:796	arg1	samples					821:827	the fecal and urine samples	801:827	samples	821:827	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	9	2	theme	rat	1295:1297	arg1	samples					1305:1311	rat urine samples	1295:1311	rat urine samples	1295:1311	However, only 7 metabolites were altered in rat urine samples.
30933879	6	3	theme	fecal	805:809	arg1	samples					821:827	the fecal and urine samples	801:827	samples	821:827	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	10	4	theme	Metabolic	1314:1322	arg1	alterations					1324:1334	Metabolic alterations	1314:1334	Metabolic alterations in fecal samples	1314:1351	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	5	5	theme	time	676:679	arg1	15 days					705:711	0, 1, 5, 10 and 15 days	689:711	0, 1, 5, 10 and 15 days	689:711	Fecal and urine samples were collected at 5 time points (0, 1, 5, 10 and 15 days).
30933879	5	5	theme	time	676:679	arg1	points					681:686	5 time points	674:686	5 time points (0, 1, 5, 10 and 15 days)	674:712	Fecal and urine samples were collected at 5 time points (0, 1, 5, 10 and 15 days).
30933879	11	6	theme	such	1547:1550	arg1	alterations					1552:1562	such alterations	1547:1562	such alterations	1547:1562	Fecal targeted metabolomics were proven to be suitable for uplifting and highlighting such alterations.
30933879	3	7	theme	metabolic	515:523	arg1	alterations					525:535	metabolic alterations	515:535	metabolic alterations	515:535	Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations.
30933879	4	8	theme	male	563:566	arg1	rats					575:578	16 male Wistar rats	560:578	16 male Wistar rats	560:578	In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period.
30933879	7	9	from	day	1067:1069	arg1	differentiation					1048:1062	only a mild differentiation	1036:1062	only a mild differentiation at day 1	1036:1071	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	2	10	theme	carob	247:251	arg1	effects					236:242	The beneficial effects	221:242	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease	221:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	10	theme	carob	247:251	arg1	few					372:374	few	372:374	few	372:374	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	6	11	theme	organic	867:873	arg1	acids					875:879	organic acids	867:879	organic acids	867:879	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	8	12	theme	organic	1236:1242	arg1	acids					1244:1248	organic acids	1236:1248	organic acids	1236:1248	Twenty-one fecal metabolites were responsible for the separation including amino acids and their derivatives, vitamins and organic acids.
30933879	8	13	theme	fecal	1124:1128	arg1	metabolites					1130:1140	Twenty-one fecal metabolites	1113:1140	Twenty-one fecal metabolites	1113:1140	Twenty-one fecal metabolites were responsible for the separation including amino acids and their derivatives, vitamins and organic acids.
30933879	1	14	theme	high	132:135	arg1	value					149:153	high nutritional value	132:153	high nutritional value	132:153	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
30933879	7	15	theme	15 days	1011:1017	arg1	treatment					1019:1027	15 days treatment	1011:1027	15 days treatment	1011:1027	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	11	16	theme	targeted	1467:1474	arg1	metabolomics					1476:1487	Fecal targeted metabolomics	1461:1487	Fecal targeted metabolomics	1461:1487	Fecal targeted metabolomics were proven to be suitable for uplifting and highlighting such alterations.
30933879	1	17	theme	Ceratonia	70:78	arg1	siliqua					80:86	Ceratonia siliqua	70:86	Ceratonia siliqua	70:86	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
30933879	1	18	theme	nutritional	137:147	arg1	value					149:153	high nutritional value	132:153	high nutritional value	132:153	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
30933879	7	19	theme	mild	1043:1046	arg1	differentiation					1048:1062	only a mild differentiation	1036:1062	only a mild differentiation at day 1	1036:1071	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	10	20	theme	fecal	1339:1343	arg1	samples					1345:1351	fecal samples	1339:1351	fecal samples	1339:1351	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	2	21	theme	reflux	346:351	arg1	disease					353:359	gastro esophageal reflux disease	328:359	gastro esophageal reflux disease	328:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	22	theme	actions	395:401	arg1	effects					236:242	The beneficial effects	221:242	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease	221:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	22	theme	actions	395:401	arg1	few					372:374	few	372:374	few	372:374	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	7	23	theme	fecal	965:969	arg1	metabolome					971:980	fecal metabolome	965:980	fecal metabolome	965:980	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	2	24	theme	metabolic	269:277	arg1	syndrome					279:286	metabolic syndrome	269:286	metabolic syndrome	269:286	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	25	theme	esophageal	335:344	arg1	disease					353:359	gastro esophageal reflux disease	328:359	gastro esophageal reflux disease	328:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	26	theme	therapeutic	383:393	arg1	actions					395:401	its therapeutic actions	379:401	its therapeutic actions	379:401	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	8	27	theme	amino	1188:1192	arg1	acids					1194:1198	amino acids	1188:1198	amino acids	1188:1198	Twenty-one fecal metabolites were responsible for the separation including amino acids and their derivatives, vitamins and organic acids.
30933879	10	28	theme	physiological	1376:1388	arg1	adaptations					1406:1416	physiological and biochemical adaptations	1376:1416	physiological and biochemical adaptations derived from the nutritional intervention	1376:1458	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	11	29	theme	Fecal	1461:1465	arg1	metabolomics					1476:1487	Fecal targeted metabolomics	1461:1487	Fecal targeted metabolomics	1461:1487	Fecal targeted metabolomics were proven to be suitable for uplifting and highlighting such alterations.
30933879	4	30	theme	carob	598:602	arg1	powder					604:609	carob powder	598:609	carob powder	598:609	In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period.
30933879	0	31	theme	fecal	10:14	arg1	samples					16:22	fecal samples	10:22	fecal samples	10:22	Urine and fecal samples targeted metabolomics of carobs treated rats.
30933879	4	32	theme	present	545:551	arg1	study					553:557	the present study	541:557	the present study	541:557	In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period.
30933879	6	33	theme	hydrophilic	760:770	arg1	metabolites					772:782	63 and 67 hydrophilic metabolites	750:782	63 and 67 hydrophilic metabolites	750:782	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	10	34	theme	nutritional	1435:1445	arg1	intervention					1447:1458	the nutritional intervention	1431:1458	the nutritional intervention	1431:1458	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	9	35	theme	urine	1299:1303	arg1	samples					1305:1311	rat urine samples	1295:1311	rat urine samples	1295:1311	However, only 7 metabolites were altered in rat urine samples.
30933879	1	36	theme	great	162:166	arg1	significance					177:188	great economic significance	162:188	great economic significance	162:188	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
30933879	7	37	theme	clear	933:937	arg1	separation					945:954	A clear group separation	931:954	A clear group separation based on fecal metabolome	931:980	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	1	38	theme	economic	168:175	arg1	significance					177:188	great economic significance	162:188	great economic significance	162:188	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
30933879	8	39	theme	Twenty-one	1113:1122	arg1	metabolites					1130:1140	Twenty-one fecal metabolites	1113:1140	Twenty-one fecal metabolites	1113:1140	Twenty-one fecal metabolites were responsible for the separation including amino acids and their derivatives, vitamins and organic acids.
30933879	3	40	theme	Metabolomics-based	404:421	arg1	analysis					423:430	Metabolomics-based analysis	404:430	Metabolomics-based analysis	404:430	Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations.
30933879	4	41	theme	15-day	617:622	arg1	period					624:629	a 15-day period	615:629	a 15-day period	615:629	In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period.
30933879	7	42	theme	group	939:943	arg1	separation					945:954	A clear group separation	931:954	A clear group separation based on fecal metabolome	931:980	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	6	43	theme	endogenous	909:918	arg1	compounds					920:928	other endogenous compounds	903:928	other endogenous compounds	903:928	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	0	44	theme	carobs	49:54	arg1	rats					64:67	carobs treated rats	49:67	carobs treated rats	49:67	Urine and fecal samples targeted metabolomics of carobs treated rats.
30933879	5	45	theme	Fecal	632:636	arg1	samples					648:654	Fecal and urine samples	632:654	Fecal and urine samples	632:654	Fecal and urine samples were collected at 5 time points (0, 1, 5, 10 and 15 days).
30933879	7	46	theme	urine	1095:1099	arg1	metabolome					1101:1110	urine metabolome	1095:1110	urine metabolome	1095:1110	A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome.
30933879	10	47	theme	biochemical	1394:1404	arg1	adaptations					1406:1416	physiological and biochemical adaptations	1376:1416	physiological and biochemical adaptations derived from the nutritional intervention	1376:1458	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	10	48	attach	derived	1418:1424	arg1	intervention					1447:1458	the nutritional intervention	1431:1458	the nutritional intervention	1431:1458	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	10	48	attach	derived	1418:1424	arg2	adaptations					1406:1416	physiological and biochemical adaptations	1376:1416	physiological and biochemical adaptations derived from the nutritional intervention	1376:1458	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	5	49	theme	urine	642:646	arg1	samples					648:654	Fecal and urine samples	632:654	Fecal and urine samples	632:654	Fecal and urine samples were collected at 5 time points (0, 1, 5, 10 and 15 days).
30933879	2	50	theme	beneficial	225:234	arg1	effects					236:242	The beneficial effects	221:242	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease	221:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	2	50	theme	beneficial	225:234	arg1	few					372:374	few	372:374	few	372:374	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	3	51	theme	ultimate	444:451	arg1	tool					453:456	an ultimate tool	441:456	an ultimate tool	441:456	Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations.
30933879	6	52	theme	other	903:907	arg1	compounds					920:928	other endogenous compounds	903:928	other endogenous compounds	903:928	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	6	53	theme	HILIC-MS/MS	730:740	arg1	method					742:747	the applied HILIC-MS/MS method	718:747	the applied HILIC-MS/MS method	718:747	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	4	54	theme	Wistar	568:573	arg1	rats					575:578	16 male Wistar rats	560:578	16 male Wistar rats	560:578	In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period.
30933879	0	55	theme	rats	64:67	arg1	metabolomics					33:44	metabolomics	33:44	metabolomics of carobs treated rats	33:67	Urine and fecal samples targeted metabolomics of carobs treated rats.
30933879	6	56	theme	applied	722:728	arg1	method					742:747	the applied HILIC-MS/MS method	718:747	the applied HILIC-MS/MS method	718:747	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	2	57	theme	gastro	328:333	arg1	disease					353:359	gastro esophageal reflux disease	328:359	gastro esophageal reflux disease	328:359	The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions.
30933879	0	58	theme	treated	56:62	arg1	rats					64:67	carobs treated rats	49:67	carobs treated rats	49:67	Urine and fecal samples targeted metabolomics of carobs treated rats.
30933879	6	59	theme	urine	815:819	arg1	samples					821:827	the fecal and urine samples	801:827	samples	821:827	By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds.
30933879	3	60	theme	nutritional	482:492	arg1	intervention					494:505	nutritional intervention	482:505	nutritional intervention derived metabolic alterations	482:535	Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations.
30933879	10	61	from	alterations	1324:1334	arg1	samples					1345:1351	fecal samples	1339:1351	fecal samples	1339:1351	Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention.
30933879	1	62	theme	unique	201:206	arg1	composition					208:218	its unique composition	197:218	its unique composition	197:218	Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition.
31616890	6	0	theme	mouse	1285:1289	arg1	model					1291:1295	a liver puncture mouse model	1268:1295	a liver puncture mouse model	1268:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	6	1	theme	untreated	1247:1255	arg1	control					1257:1263	the untreated control	1243:1263	the untreated control in a liver puncture mouse model	1243:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	9	2	theme	co-assembled	1614:1625	arg1	nanostructures					1627:1640	co-assembled nanostructures	1614:1640	co-assembled nanostructures composed of biofunctional peptides and polysaccharides	1614:1695	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	3	3	theme	mechanical	590:599	arg1	properties					601:610	excellent mechanical properties	580:610	excellent mechanical properties	580:610	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	3	4	theme	excellent	580:588	arg1	properties					601:610	excellent mechanical properties	580:610	excellent mechanical properties	580:610	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	4	5	theme	better	901:906	arg1	strength					919:926	better mechanical strength	901:926	better mechanical strength	901:926	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	7	6	theme	mice	1464:1467	arg1	model					1455:1459	a full-thickness skin defect model	1426:1459	a full-thickness skin defect model of mice	1426:1467	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	1	7	theme	non-compressive	193:207	arg1	management					215:224	non-compressive wound management	193:224	non-compressive wound management	193:224	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	9	8	theme	nanocomposite	1754:1766	arg1	materials					1768:1776	nanocomposite materials	1754:1776	nanocomposite materials for a variety of biomedical applications	1754:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	9	9	theme	applications	1806:1817	arg1	variety					1784:1790	a variety	1782:1790	a variety of biomedical applications	1782:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	9	9	theme	applications	1806:1817	arg1	applications					1806:1817	biomedical applications	1795:1817	biomedical applications	1795:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	4	10	theme	co-assembling	719:731	arg1	process					733:739	The co-assembling process	715:739	The co-assembling process	715:739	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	3	11	theme	high	616:619	arg1	efficacy					621:628	high efficacy	616:628	high efficacy	616:628	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	6	12	theme	bleeding	1223:1230	arg1	bleeding					1223:1230	bleeding	1223:1230	bleeding	1223:1230	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	6	12	theme	bleeding	1223:1230	arg1	amount					1213:1218	the amount	1209:1218	the amount of bleeding	1209:1230	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	1	13	theme	Injectable	128:137	arg1	hydrogels					139:147	Injectable hydrogels	128:147	Injectable hydrogels	128:147	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	1	13	theme	Injectable	128:137	arg1	materials					163:171	promising materials	153:171	promising materials for applications in non-compressive wound management	153:224	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	3	14	theme	hemostatic	638:647	arg1	control					649:655	hemostatic control	638:655	hemostatic control	638:655	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	9	15	theme	biomedical	1795:1804	arg1	applications					1806:1817	biomedical applications	1795:1817	biomedical applications	1795:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	8	16	theme	Pept-1/ALG	1487:1496	arg1	hydrogel					1498:1505	the Pept-1/ALG hydrogel	1483:1505	the Pept-1/ALG hydrogel	1483:1505	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	7	17	theme	healing	1415:1421	arg1	rate					1401:1404	the rate	1397:1404	the rate of wound healing	1397:1421	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	5	18	theme	Pept-1/ALG	980:989	arg1	hydrogels					991:999	As-prepared Pept-1/ALG hydrogels	968:999	As-prepared Pept-1/ALG hydrogels	968:999	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	2	19	theme	materials	303:311	arg1	fabrication					259:269	the fabrication	255:269	the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors	255:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	6	20	theme	liver	1270:1274	arg1	model					1291:1295	a liver puncture mouse model	1268:1295	a liver puncture mouse model	1268:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	2	21	theme	hydrogel	294:301	arg1	materials					303:311	mechanically stable hydrogel materials	274:311	mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors	274:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	8	22	theme	biocompatibility	1524:1539	arg1	activity					1567:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	6	23	from	model	1291:1295	arg1	control					1257:1263	the untreated control	1243:1263	the untreated control in a liver puncture mouse model	1243:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	6	23	from	model	1291:1295	arg1	%					1238:1238	∼18%	1235:1238	∼18% of the untreated control in a liver puncture mouse model	1235:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	0	24	theme	alginate	67:74	arg1	hydrogels					28:36	Co-assembled supramolecular hydrogels	0:36	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.	0:126	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	4	25	with	strength	919:926	arg1	structures					886:895	denser nanofibrillar structures	865:895	denser nanofibrillar structures	865:895	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	6	26	from	control	1257:1263	arg1	model					1291:1295	a liver puncture mouse model	1268:1295	a liver puncture mouse model	1268:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	4	27	with	hydrogel	851:858	arg1	structures					886:895	denser nanofibrillar structures	865:895	denser nanofibrillar structures	865:895	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	0	28	theme	rapid	80:84	arg1	hemostasis					86:95	rapid hemostasis	80:95	rapid hemostasis	80:95	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	8	29	theme	hemolytic	1557:1565	arg1	activity					1567:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	4	30	theme	metal	813:817	arg1	chelation					819:827	metal chelation	813:827	metal chelation	813:827	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	6	31	from	%	1238:1238	arg1	model					1291:1295	a liver puncture mouse model	1268:1295	a liver puncture mouse model	1268:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	3	32	theme	adhesive	484:491	arg1	Pept-1					512:517	Pept-1	512:517	Pept-1	512:517	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	3	32	theme	adhesive	484:491	arg1	conjugate					501:509	a cell adhesive peptide conjugate	477:509	a cell adhesive peptide conjugate (Pept-1)	477:518	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	0	33	theme	wound	113:117	arg1	healing					119:125	efficacious wound healing	101:125	efficacious wound healing	101:125	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	0	34	theme	Co-assembled	0:11	arg1	hydrogels					28:36	Co-assembled supramolecular hydrogels	0:36	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.	0:126	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	5	35	theme	injectability	1021:1033	arg1	properties					1051:1060	excellent injectability and thixotropic properties	1011:1060	excellent injectability and thixotropic properties	1011:1060	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	3	36	theme	supramolecular	550:563	arg1	hydrogels					565:573	supramolecular hydrogels	550:573	supramolecular hydrogels with excellent mechanical properties and high efficacy	550:628	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	5	37	theme	thixotropic	1039:1049	arg1	properties					1051:1060	excellent injectability and thixotropic properties	1011:1060	excellent injectability and thixotropic properties	1011:1060	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	6	38	theme	fast	1142:1145	arg1	hemostasis					1147:1156	fast hemostasis	1142:1156	fast hemostasis	1142:1156	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	3	39	theme	growth	699:704	arg1	factors					706:712	no additional growth factors	685:712	no additional growth factors	685:712	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	4	40	theme	denser	865:870	arg1	structures					886:895	denser nanofibrillar structures	865:895	denser nanofibrillar structures	865:895	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	3	41	theme	conjugate	501:509	arg1	co-assembly					462:472	the co-assembly	458:472	the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG)	458:537	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	0	42	theme	cell	41:44	arg1	peptide					55:61	cell adhesive peptide	41:61	cell adhesive peptide	41:61	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	3	43	with	hydrogels	565:573	arg1	properties					601:610	excellent mechanical properties	580:610	excellent mechanical properties	580:610	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	3	43	with	hydrogels	565:573	arg1	efficacy					621:628	high efficacy	616:628	high efficacy	616:628	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	7	44	theme	NIH3T3	1358:1363	arg1	cells					1365:1369	fibroblast NIH3T3 cells	1347:1369	fibroblast NIH3T3 cells	1347:1369	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	2	45	theme	additional	396:405	arg1	supplements					407:417	additional supplements	396:417	additional supplements of growth factors	396:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	0	46	theme	peptide	55:61	arg1	hydrogels					28:36	Co-assembled supramolecular hydrogels	0:36	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.	0:126	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	2	47	theme	factors	429:435	arg1	supplements					407:417	additional supplements	396:417	additional supplements of growth factors	396:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	5	48	theme	wound	1095:1099	arg1	dressing					1101:1108	wound dressing	1095:1108	wound dressing	1095:1108	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	9	49	theme	broad	1739:1743	arg1	range					1745:1749	a broad range	1737:1749	a broad range of nanocomposite materials for a variety of biomedical applications	1737:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	2	50	theme	inherent	318:325	arg1	functionalities					327:341	inherent functionalities	318:341	inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors	318:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	1	51	theme	wound	209:213	arg1	management					215:224	non-compressive wound management	193:224	non-compressive wound management	193:224	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	4	52	theme	mechanical	908:917	arg1	strength					919:926	better mechanical strength	901:926	better mechanical strength	901:926	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	2	53	theme	hemostatic	351:360	arg1	control					362:368	hemostatic control	351:368	hemostatic control	351:368	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	7	54	theme	defect	1448:1453	arg1	model					1455:1459	a full-thickness skin defect model	1426:1459	a full-thickness skin defect model of mice	1426:1467	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	9	55	theme	materials	1768:1776	arg1	range					1745:1749	a broad range	1737:1749	a broad range of nanocomposite materials for a variety of biomedical applications	1737:1817	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	7	56	theme	full-thickness	1428:1441	arg1	model					1455:1459	a full-thickness skin defect model	1426:1459	a full-thickness skin defect model of mice	1426:1467	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	5	57	theme	As-prepared	968:978	arg1	hydrogels					991:999	As-prepared Pept-1/ALG hydrogels	968:999	As-prepared Pept-1/ALG hydrogels	968:999	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	2	58	with	materials	303:311	arg1	functionalities					327:341	inherent functionalities	318:341	inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors	318:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	4	59	theme	Pept-1	950:955	arg1	gel					957:959	the Pept-1 gel	946:959	the Pept-1 gel alone	946:965	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	8	60	theme	excellent	1514:1522	arg1	activity					1567:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	6	61	theme	puncture	1276:1283	arg1	model					1291:1295	a liver puncture mouse model	1268:1295	a liver puncture mouse model	1268:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	4	62	theme	ALG	755:757	arg1	process					733:739	The co-assembling process	715:739	The co-assembling process	715:739	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	3	63	theme	wound	661:665	arg1	healing					667:673	wound healing	661:673	wound healing	661:673	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	7	64	theme	skin	1443:1446	arg1	model					1455:1459	a full-thickness skin defect model	1426:1459	a full-thickness skin defect model of mice	1426:1467	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	6	65	theme	composite	1115:1123	arg1	hydrogel					1125:1132	The composite hydrogel	1111:1132	The composite hydrogel	1111:1132	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	8	66	theme	with	1541:1544	arg1	activity					1567:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	7	67	theme	wound	1409:1413	arg1	healing					1415:1421	wound healing	1409:1421	wound healing	1409:1421	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	2	68	theme	stable	287:292	arg1	materials					303:311	mechanically stable hydrogel materials	274:311	mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors	274:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	1	69	theme	promising	153:161	arg1	hydrogels					139:147	Injectable hydrogels	128:147	Injectable hydrogels	128:147	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	1	69	theme	promising	153:161	arg1	materials					163:171	promising materials	153:171	promising materials for applications in non-compressive wound management	153:224	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	2	70	from	functionalities	327:341	arg1	control					362:368	hemostatic control	351:368	hemostatic control	351:368	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	2	70	from	functionalities	327:341	arg1	healing					380:386	wound healing	374:386	wound healing	374:386	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	8	71	theme	obvious	1549:1555	arg1	activity					1567:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	excellent biocompatibility with no obvious hemolytic activity	1514:1574	In addition, the Pept-1/ALG hydrogel showed excellent biocompatibility with no obvious hemolytic activity.
31616890	6	72	theme	control	1257:1263	arg1	control					1257:1263	the untreated control	1243:1263	the untreated control in a liver puncture mouse model	1243:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	6	72	theme	control	1257:1263	arg1	%					1238:1238	∼18%	1235:1238	∼18% of the untreated control in a liver puncture mouse model	1235:1295	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	4	73	theme	Pept-1	744:749	arg1	process					733:739	The co-assembling process	715:739	The co-assembling process	715:739	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	0	74	theme	efficacious	101:111	arg1	healing					119:125	efficacious wound healing	101:125	efficacious wound healing	101:125	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	1	75	from	applications	177:188	arg1	management					215:224	non-compressive wound management	193:224	non-compressive wound management	193:224	Injectable hydrogels are promising materials for applications in non-compressive wound management.
31616890	2	76	theme	wound	374:378	arg1	healing					380:386	wound healing	374:386	wound healing	374:386	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31616890	6	77	theme	whole	1175:1179	arg1	blood					1181:1185	whole blood	1175:1185	whole blood	1175:1185	The composite hydrogel induced fast hemostasis when spiked with whole blood in vitro, and reduced the amount of bleeding to ∼18% of the untreated control in a liver puncture mouse model.
31616890	3	78	theme	peptide	493:499	arg1	Pept-1					512:517	Pept-1	512:517	Pept-1	512:517	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	3	78	theme	peptide	493:499	arg1	conjugate					501:509	a cell adhesive peptide conjugate	477:509	a cell adhesive peptide conjugate (Pept-1)	477:518	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	0	79	theme	supramolecular	13:26	arg1	hydrogels					28:36	Co-assembled supramolecular hydrogels	0:36	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.	0:126	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	5	80	theme	excellent	1011:1019	arg1	properties					1051:1060	excellent injectability and thixotropic properties	1011:1060	excellent injectability and thixotropic properties	1011:1060	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	3	81	theme	cell	479:482	arg1	Pept-1					512:517	Pept-1	512:517	Pept-1	512:517	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	3	81	theme	cell	479:482	arg1	conjugate					501:509	a cell adhesive peptide conjugate	477:509	a cell adhesive peptide conjugate (Pept-1)	477:518	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	7	82	theme	cells	1365:1369	arg1	migration					1334:1342	migration	1334:1342	migration	1334:1342	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	7	82	theme	cells	1365:1369	arg1	adhesion					1321:1328	adhesion	1321:1328	adhesion	1321:1328	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	3	83	theme	additional	688:697	arg1	factors					706:712	no additional growth factors	685:712	no additional growth factors	685:712	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	4	84	theme	electrostatic	782:794	arg1	interactions					796:807	electrostatic interactions	782:807	electrostatic interactions	782:807	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	0	85	theme	adhesive	46:53	arg1	peptide					55:61	cell adhesive peptide	41:61	cell adhesive peptide	41:61	Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing.
31616890	9	86	theme	biofunctional	1654:1666	arg1	peptides					1668:1675	biofunctional peptides	1654:1675	biofunctional peptides	1654:1675	In future, the strategy of utilizing co-assembled nanostructures composed of biofunctional peptides and polysaccharides could be further exploited to construct a broad range of nanocomposite materials for a variety of biomedical applications.
31616890	7	87	theme	fibroblast	1347:1356	arg1	cells					1365:1369	fibroblast NIH3T3 cells	1347:1369	fibroblast NIH3T3 cells	1347:1369	Meanwhile, it promoted adhesion and migration of fibroblast NIH3T3 cells in vitro, and accelerated the rate of wound healing in a full-thickness skin defect model of mice.
31616890	4	88	theme	nanofibrillar	872:884	arg1	structures					886:895	denser nanofibrillar structures	865:895	denser nanofibrillar structures	865:895	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	5	89	theme	ideal	1075:1079	arg1	materials					1081:1089	ideal materials	1075:1089	ideal materials for wound dressing	1075:1108	As-prepared Pept-1/ALG hydrogels exhibited excellent injectability and thixotropic properties, making them ideal materials for wound dressing.
31616890	3	90	theme	alginate	524:531	arg1	co-assembly					462:472	the co-assembly	458:472	the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG)	458:537	Herein, we reported the co-assembly of a cell adhesive peptide conjugate (Pept-1) and alginate (ALG), to confer supramolecular hydrogels with excellent mechanical properties and high efficacy in both hemostatic control and wound healing requiring no additional growth factors.
31616890	4	91	theme	composite	841:849	arg1	hydrogel					851:858	a composite hydrogel	839:858	a composite hydrogel with denser nanofibrillar structures	839:895	The co-assembling process of Pept-1 and ALG, which was mediated by electrostatic interactions and metal chelation, afforded a composite hydrogel with denser nanofibrillar structures and better mechanical strength when comparing to the Pept-1 gel alone.
31616890	2	92	theme	growth	422:427	arg1	factors					429:435	growth factors	422:435	growth factors	422:435	Yet difficulties remain for the fabrication of mechanically stable hydrogel materials with inherent functionalities in both hemostatic control and wound healing without additional supplements of growth factors.
31173023	0	0	theme	endophytic	82:91	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	6	1	dep	mechanism	1000:1008	arg1	binding					1037:1043	(1) binding	1033:1043	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	1	dep	mechanism	1000:1008	arg1	adapt					1013:1017	adapt	1013:1017	to adapt to Cd stress	1010:1030	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	1	dep	mechanism	1000:1008	arg1	sequestration					1192:1204	(2) intracellular sequestration	1174:1204	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	1	dep	mechanism	1000:1008	arg1	alleviation					1287:1297	(4) alleviation	1283:1297	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	1	dep	mechanism	1000:1008	arg1	excretion					1236:1244	(3) excretion	1232:1244	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	2	theme	biochemical	816:826	arg1	detection					828:836	biochemical detection	816:836	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	3	theme	label-free	896:905	arg1	analysis					938:945	label-free quantitative proteomic profile analysis	896:945	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	4	theme	physiological	714:726	arg1	observation					728:738	physiological observation	714:738	physiological observation	714:738	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	7	5	theme	phytoremediation	1469:1484	arg1	efficiency					1486:1495	phytoremediation efficiency	1469:1495	phytoremediation efficiency	1469:1495	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	6	6	theme	efflux	1269:1274	arg1	pumps					1276:1280	efflux pumps	1269:1280	efflux pumps	1269:1280	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	1	7	theme	endophytic	171:180	arg1	YG-3					192:195	An endophytic bacterium YG-3	168:195	An endophytic bacterium YG-3 with high cadmium (Cd) resistance	168:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	6	8	dep	sequestration	1192:1204	arg1	2					1175:1175	2	1175:1175	2	1175:1175	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	0	9	theme	Burkholderia	103:114	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	6	10	from	cell	1261:1264	arg1	Cd					1249:1250	Cd	1249:1250	Cd from the cell	1249:1264	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	10	from	cell	1261:1264	arg1	excretion					1236:1244	(3) excretion	1232:1244	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	11	theme	global	634:639	arg1	mechanisms					652:661	The global resistance mechanisms	630:661	The global resistance mechanisms of YG-3	630:669	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	0	12	theme	bacterium	93:101	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	5	13	theme	resistance	641:650	arg1	mechanisms					652:661	The global resistance mechanisms	630:661	The global resistance mechanisms of YG-3	630:669	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	3	14	theme	L-1	518:520	arg1	concentrations					480:493	high concentrations	475:493	high concentrations of both 100 and 500 mg L-1	475:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	4	15	theme	adsorption	557:566	arg1	strategies					568:577	adsorption strategies	557:577	adsorption strategies	557:577	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	0	16	theme	strain	128:133	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	6	17	theme	wall	1100:1103	arg1	components					1105:1114	the cell wall components	1091:1114	the cell wall components	1091:1114	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	18	theme	secreted	1128:1135	arg1	siderophores					1137:1148	secreted siderophores	1128:1148	secreted siderophores	1128:1148	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	7	19	theme	heavy	1500:1504	arg1	sites					1525:1529	heavy metal-contaminated sites	1500:1529	heavy metal-contaminated sites	1500:1529	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	1	20	theme	bacterium	182:190	arg1	YG-3					192:195	An endophytic bacterium YG-3	168:195	An endophytic bacterium YG-3 with high cadmium (Cd) resistance	168:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	0	21	theme	cenocepacia	116:126	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	5	22	theme	quantitative	907:918	arg1	analysis					938:945	label-free quantitative proteomic profile analysis	896:945	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	3	23	with	solution	461:468	arg1	concentrations					480:493	high concentrations	475:493	high concentrations of both 100 and 500 mg L-1	475:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	5	24	theme	electron	795:802	arg1	microscopy					804:813	transmission electron microscopy	782:813	transmission electron microscopy	782:813	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	6	25	dep	excretion	1236:1244	arg1	3					1233:1233	3	1233:1233	3	1233:1233	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	26	theme	transmission	782:793	arg1	microscopy					804:813	transmission electron microscopy	782:813	transmission electron microscopy	782:813	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	6	27	dep	binding	1037:1043	arg1	1					1034:1034	1	1034:1034	1	1034:1034	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	28	theme	complex	992:998	arg1	mechanism					1000:1008	a complex mechanism	990:1008	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	29	theme	several	692:698	arg1	microscopy					767:776	scanning electron microscopy	749:776	scanning electron microscopy	749:776	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	29	theme	several	692:698	arg1	microscopy					804:813	transmission electron microscopy	782:813	transmission electron microscopy	782:813	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	29	theme	several	692:698	arg1	levels					700:705	several levels	692:705	several levels	692:705	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	0	30	theme	Physiological	0:12	arg1	insight					41:47	Physiological, biochemical and proteomic insight	0:47	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.	0:166	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	1	31	theme	composite	267:275	arg1	tailing					282:288	a composite mine tailing	265:288	a composite mine tailing	265:288	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	6	32	theme	Cd	1209:1210	arg1	sequestration					1192:1204	(2) intracellular sequestration	1174:1204	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	33	theme	compound	849:856	arg1	production					858:867	active compound production	842:867	active compound production	842:867	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	6	34	dep	alleviation	1287:1297	arg1	4					1284:1284	4	1284:1284	4	1284:1284	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	1	35	theme	high	202:205	arg1	resistance					220:229	high cadmium (Cd) resistance	202:229	high cadmium (Cd) resistance	202:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	1	36	theme	mine	277:280	arg1	tailing					282:288	a composite mine tailing	265:288	a composite mine tailing	265:288	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	0	37	theme	response	140:147	arg1	strategies					65:74	integrated strategies	54:74	integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	54:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	1	38	theme	cadmium	207:213	arg1	resistance					220:229	high cadmium (Cd) resistance	202:229	high cadmium (Cd) resistance	202:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	0	39	theme	biochemical	15:25	arg1	insight					41:47	Physiological, biochemical and proteomic insight	0:47	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.	0:166	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	4	40	theme	various	534:540	arg1	absorption					542:551	various absorption	534:551	various absorption	534:551	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	0	41	theme	YG-3	135:138	arg1	response					140:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response	79:147	an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	79:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	2	42	theme	genomic	346:352	arg1	analyses					385:392	genomic, physiological and biochemical analyses	346:392	genomic, physiological and biochemical analyses	346:392	It was identified as Burkholderia cenocepacia based on genomic, physiological and biochemical analyses.
31173023	3	43	theme	Cd	450:451	arg1	solution					461:468	Cd aqueous solution	450:468	Cd aqueous solution with high concentrations of both 100 and 500 mg L-1	450:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	5	44	theme	scanning	749:756	arg1	microscopy					767:776	scanning electron microscopy	749:776	scanning electron microscopy	749:776	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	45	theme	profile	930:936	arg1	analysis					938:945	label-free quantitative proteomic profile analysis	896:945	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	0	46	theme	cadmium	152:158	arg1	stress					160:165	cadmium stress	152:165	cadmium stress	152:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	6	47	theme	cell	1095:1098	arg1	components					1105:1114	the cell wall components	1091:1114	the cell wall components	1091:1114	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	2	48	theme	biochemical	373:383	arg1	analyses					385:392	genomic, physiological and biochemical analyses	346:392	genomic, physiological and biochemical analyses	346:392	It was identified as Burkholderia cenocepacia based on genomic, physiological and biochemical analyses.
31173023	7	49	theme	endophyte	1360:1368	arg1	YG-3					1370:1373	endophyte YG-3	1360:1373	endophyte YG-3	1360:1373	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	0	50	theme	proteomic	31:39	arg1	insight					41:47	Physiological, biochemical and proteomic insight	0:47	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.	0:166	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	6	51	theme	Cd	1302:1303	arg1	toxicity					1305:1312	Cd toxicity	1302:1312	Cd toxicity	1302:1312	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	52	theme	electron	758:765	arg1	microscopy					767:776	scanning electron microscopy	749:776	scanning electron microscopy	749:776	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	4	53	located	found	584:588	arg2	absorption					542:551	various absorption	534:551	various absorption	534:551	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	4	53	located	found	584:588	arg2	Meanwhile					523:531	Meanwhile	523:531	Meanwhile	523:531	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	4	53	located	found	584:588	arg1	concentrations					614:627	the two different Cd concentrations	593:627	the two different Cd concentrations	593:627	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	4	53	located	found	584:588	arg2	strategies					568:577	adsorption strategies	557:577	adsorption strategies	557:577	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	4	54	theme	Cd	611:612	arg1	concentrations					614:627	the two different Cd concentrations	593:627	the two different Cd concentrations	593:627	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	0	55	theme	integrated	54:63	arg1	strategies					65:74	integrated strategies	54:74	integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress	54:165	Physiological, biochemical and proteomic insight into integrated strategies of an endophytic bacterium Burkholderia cenocepacia strain YG-3 response to cadmium stress.
31173023	5	56	dep	levels	700:705	arg1	analysis					938:945	label-free quantitative proteomic profile analysis	896:945	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	5	56	dep	levels	700:705	arg1	detection					828:836	biochemical detection	816:836	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	6	57	theme	intracellular	1178:1190	arg1	sequestration					1192:1204	(2) intracellular sequestration	1174:1204	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	3	58	from	%	445:445	arg1	solution					461:468	Cd aqueous solution	450:468	Cd aqueous solution with high concentrations of both 100 and 500 mg L-1	450:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	5	59	theme	proteomic	920:928	arg1	analysis					938:945	label-free quantitative proteomic profile analysis	896:945	several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis	692:945	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	3	60	theme	removal	402:408	arg1	rate					410:413	The Cd removal rate	395:413	The Cd removal rate by YG-3	395:421	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	6	61	from	excretion	1236:1244	arg1	cell					1261:1264	the cell	1257:1264	the cell	1257:1264	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	1	62	attach	isolated	235:242	arg1	poplar					249:254	poplar	249:254	poplar grown in a composite mine tailing	249:288	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	1	62	attach	isolated	235:242	arg2	YG-3					192:195	An endophytic bacterium YG-3	168:195	An endophytic bacterium YG-3 with high cadmium (Cd) resistance	168:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	3	63	theme	high	475:478	arg1	concentrations					480:493	high concentrations	475:493	high concentrations of both 100 and 500 mg L-1	475:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	7	64	contain	has	1417:1419	arg1	YG-3					1370:1373	endophyte YG-3	1360:1373	endophyte YG-3	1360:1373	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	7	64	contain	has	1417:1419	arg2	potential					1421:1429	potential	1421:1429	potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites	1421:1529	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	5	65	theme	YG-3	666:669	arg1	mechanisms					652:661	The global resistance mechanisms	630:661	The global resistance mechanisms of YG-3	630:669	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	6	66	contain	possesses	980:988	arg2	mechanism					1000:1008	a complex mechanism	990:1008	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	6	66	contain	possesses	980:988	arg1	YG-3					975:978	YG-3	975:978	YG-3	975:978	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	3	67	theme	aqueous	453:459	arg1	solution					461:468	Cd aqueous solution	450:468	Cd aqueous solution with high concentrations of both 100 and 500 mg L-1	450:520	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	5	68	theme	infrared	873:880	arg1	spectroscopy					882:893	infrared spectroscopy	873:893	infrared spectroscopy	873:893	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	7	69	theme	metal-contaminated	1506:1523	arg1	sites					1525:1529	heavy metal-contaminated sites	1500:1529	heavy metal-contaminated sites	1500:1529	Our results demonstrate that endophyte YG-3 is well adjusted to largely remove Cd and has potential to cooperate with its host to improve phytoremediation efficiency in heavy metal-contaminated sites.
31173023	1	70	with	YG-3	192:195	arg1	resistance					220:229	high cadmium (Cd) resistance	202:229	high cadmium (Cd) resistance	202:229	An endophytic bacterium YG-3 with high cadmium (Cd) resistance was isolated from poplar grown in a composite mine tailing.
31173023	6	71	theme	Cd	1048:1049	arg1	binding					1037:1043	(1) binding	1033:1043	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	3	72	dep	L-1	518:520	arg1	mg					515:516	mg	515:516	mg	515:516	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	3	73	dep	mg	515:516	arg1	500					511:513	500	511:513	500	511:513	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	3	73	dep	mg	515:516	arg1	100					503:505	100	503:505	100	503:505	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	6	74	theme	toxicity	1305:1312	arg1	alleviation					1287:1297	(4) alleviation	1283:1297	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	5	75	theme	active	842:847	arg1	production					858:867	active compound production	842:867	active compound production	842:867	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	4	76	theme	different	601:609	arg1	concentrations					614:627	the two different Cd concentrations	593:627	the two different Cd concentrations	593:627	Meanwhile, various absorption and adsorption strategies were found in the two different Cd concentrations.
31173023	6	77	theme	Cd	1249:1250	arg1	excretion					1236:1244	(3) excretion	1232:1244	a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants	990:1328	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31173023	3	78	theme	Cd	399:400	arg1	rate					410:413	The Cd removal rate	395:413	The Cd removal rate by YG-3	395:421	The Cd removal rate by YG-3 could reach about 60.0% in Cd aqueous solution with high concentrations of both 100 and 500 mg L-1.
31173023	5	79	dep	observation	728:738	arg1	i.e.					708:711	i.e.	708:711	i.e.	708:711	The global resistance mechanisms of YG-3 were investigated in several levels, i.e., physiological observation, such as scanning electron microscopy and transmission electron microscopy; biochemical detection for active compound production and infrared spectroscopy; label-free quantitative proteomic profile analysis.
31173023	2	80	theme	physiological	355:367	arg1	analyses					385:392	genomic, physiological and biochemical analyses	346:392	genomic, physiological and biochemical analyses	346:392	It was identified as Burkholderia cenocepacia based on genomic, physiological and biochemical analyses.
31173023	6	81	theme	Cd	1022:1023	arg1	stress					1025:1030	Cd stress	1022:1030	Cd stress	1022:1030	The results indicated that YG-3 possesses a complex mechanism to adapt to Cd stress: (1) binding of Cd to prevent it from entering the cell by the cell wall components, as well as secreted siderophores and exopolysaccharides; (2) intracellular sequestration of Cd by metalloproteins; (3) excretion of Cd from the cell by efflux pumps; (4) alleviation of Cd toxicity by antioxidants.
31989986	3	0	theme	1663.82 ± 22.46 L/m2·h·bar	594:619	arg1	diameter					541:548	an average pore diameter	525:548	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	0	theme	1663.82 ± 22.46 L/m2·h·bar	594:619	arg1	flux					586:589	a high water flux	573:589	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	6	1	theme	CV	907:908	arg1	removal					910:916	CV removal	907:916	CV removal	907:916	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	1	2	theme	composite	167:175	arg1	membrane					177:184	a new inorganic-organic composite membrane	143:184	a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method	143:238	This study reports a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method.
31989986	0	3	theme	membrane	90:97	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.	0:122	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	3	4	theme	water	580:584	arg1	flux					586:589	a high water flux	573:589	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	5	theme	CS	442:443	arg1	solution					445:452	1.0% CS solution	437:452	1.0% CS solution	437:452	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	0	6	theme	dye	111:113	arg1	removal					115:121	aquatic dye removal	103:121	aquatic dye removal	103:121	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	4	7	theme	temperature	678:688	arg1	effects					626:632	The effects	622:632	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG	622:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	3	8	theme	solution	445:452	arg1	2.0 mL					427:432	2.0 mL	427:432	2.0 mL of 1.0% CS solution	427:452	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	9	theme	obtained	460:467	arg1	membrane					475:482	the obtained CS/PG membrane	456:482	the obtained CS/PG membrane	456:482	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	0	10	theme	aquatic	103:109	arg1	removal					115:121	aquatic dye removal	103:121	aquatic dye removal	103:121	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	8	11	theme	practical	1201:1209	arg1	applications					1211:1222	practical applications	1201:1222	practical applications in water purification	1201:1244	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	3	12	theme	CS/PG	469:473	arg1	membrane					475:482	the obtained CS/PG membrane	456:482	the obtained CS/PG membrane	456:482	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	8	13	theme	cost-saving	1148:1158	arg1	membranes					1187:1195	cost-saving and eco-friendly composite membranes	1148:1195	cost-saving and eco-friendly composite membranes	1148:1195	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	3	14	theme	CS	423:424	arg1	dosage					413:418	optimum dosage	405:418	optimum dosage of CS (2.0 mL of 1.0% CS solution)	405:453	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	8	15	theme	water	1227:1231	arg1	purification					1233:1244	water purification	1227:1244	water purification	1227:1244	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	4	16	theme	solution	702:709	arg1	temperature					678:688	temperature	678:688	temperature of the feed solution	678:709	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	16	theme	solution	702:709	arg1	pH					660:661	pH	660:661	pH	660:661	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	16	theme	solution	702:709	arg1	rate					669:672	flow rate	664:672	flow rate	664:672	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	16	theme	solution	702:709	arg1	concentration					645:657	initial concentration	637:657	initial concentration	637:657	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	8	17	theme	composite	1177:1185	arg1	membranes					1187:1195	cost-saving and eco-friendly composite membranes	1148:1195	cost-saving and eco-friendly composite membranes	1148:1195	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	6	18	theme	adsorption	998:1007	arg1	effect					974:979	the synergistic effect	958:979	the synergistic effect of rejection and adsorption	958:1007	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	4	19	theme	rate	669:672	arg1	effects					626:632	The effects	622:632	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG	622:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	6	20	theme	CS/PG	925:929	arg1	membrane					931:938	the CS/PG membrane	921:938	the CS/PG membrane	921:938	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	5	21	theme	14 h	880:883	arg1	continuous					857:866	continuous	857:866	continuous	857:866	The results indicated ~95% CV could be removed from water during continuous treating of 14 h.
31989986	7	22	theme	prolonged	1078:1086	arg1	use					1088:1090	prolonged use	1078:1090	prolonged use	1078:1090	Furthermore, the composite membrane could be easily regenerated for prolonged use.
31989986	6	23	from	effectiveness	890:902	arg1	removal					910:916	CV removal	907:916	CV removal	907:916	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	4	24	theme	concentration	645:657	arg1	effects					626:632	The effects	622:632	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG	622:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	7	25	theme	composite	1027:1035	arg1	membrane					1037:1044	the composite membrane	1023:1044	the composite membrane	1023:1044	Furthermore, the composite membrane could be easily regenerated for prolonged use.
31989986	0	26	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.	0:122	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	3	27	theme	high	496:499	arg1	porosity					501:508	a high porosity	494:508	a high porosity of 50.97%	494:518	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	4	28	theme	flow	664:667	arg1	rate					669:672	flow rate	664:672	flow rate	664:672	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	2	29	theme	"	351:351	arg1	chitosan					328:335	chitosan	328:335	chitosan (CS)	328:340	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	29	theme	"	351:351	arg1	biomaterial					353:363	a "green" biomaterial	343:363	a "green" biomaterial	343:363	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	4	30	theme	initial	637:643	arg1	concentration					645:657	initial concentration	637:657	initial concentration	637:657	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	31	theme	crystal	714:720	arg1	CV					730:731	CV	730:731	CV	730:731	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	31	theme	crystal	714:720	arg1	violet					722:727	crystal violet	714:727	crystal violet (CV) removal by the CS/PG	714:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	4	32	theme	pH	660:661	arg1	effects					626:632	The effects	622:632	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG	622:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	1	33	theme	self-assembling	217:231	arg1	method					233:238	an electrostatic self-assembling method	200:238	an electrostatic self-assembling method	200:238	This study reports a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method.
31989986	2	34	theme	active	386:391	arg1	layer					393:397	an active layer	383:397	an active layer	383:397	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	5	35	theme	~95	814:816	arg1	%					817:817	%	817:817	%	817:817	The results indicated ~95% CV could be removed from water during continuous treating of 14 h.
31989986	3	36	theme	13.93 nm	553:560	arg1	diameter					541:548	an average pore diameter	525:548	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	36	theme	13.93 nm	553:560	arg1	flux					586:589	a high water flux	573:589	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	1	37	theme	new	145:147	arg1	membrane					177:184	a new inorganic-organic composite membrane	143:184	a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method	143:238	This study reports a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method.
31989986	3	38	theme	%	440:440	arg1	solution					445:452	1.0% CS solution	437:452	1.0% CS solution	437:452	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	5	39	attach	removed	831:837	arg2	results					796:802	The results	792:802	The results indicated ~95% CV	792:820	The results indicated ~95% CV could be removed from water during continuous treating of 14 h.
31989986	5	39	attach	removed	831:837	arg1	water					844:848	water	844:848	water	844:848	The results indicated ~95% CV could be removed from water during continuous treating of 14 h.
31989986	2	40	theme	porous	271:276	arg1	PG					290:291	PG	290:291	PG	290:291	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	40	theme	porous	271:276	arg1	support					310:316	a support	308:316	a support	308:316	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	40	theme	porous	271:276	arg1	geopolymer					278:287	The low-cost and eco-friendly porous geopolymer	241:287	The low-cost and eco-friendly porous geopolymer (PG)	241:292	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	1	41	theme	electrostatic	203:215	arg1	method					233:238	an electrostatic self-assembling method	200:238	an electrostatic self-assembling method	200:238	This study reports a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method.
31989986	3	42	theme	optimum	405:411	arg1	dosage					413:418	optimum dosage	405:418	optimum dosage of CS (2.0 mL of 1.0% CS solution)	405:453	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	0	43	theme	low-cost	24:31	arg1	membrane					90:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	8	44	theme	eco-friendly	1164:1175	arg1	membranes					1187:1195	cost-saving and eco-friendly composite membranes	1148:1195	cost-saving and eco-friendly composite membranes	1148:1195	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	2	45	theme	eco-friendly	258:269	arg1	PG					290:291	PG	290:291	PG	290:291	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	45	theme	eco-friendly	258:269	arg1	support					310:316	a support	308:316	a support	308:316	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	45	theme	eco-friendly	258:269	arg1	geopolymer					278:287	The low-cost and eco-friendly porous geopolymer	241:287	The low-cost and eco-friendly porous geopolymer (PG)	241:292	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	46	used	used	370:373	arg2	chitosan					328:335	chitosan	328:335	chitosan (CS)	328:340	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	46	used	used	370:373	arg2	biomaterial					353:363	a "green" biomaterial	343:363	a "green" biomaterial	343:363	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	46	used	used	370:373	arg2	CS					338:339	CS	338:339	CS	338:339	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	8	47	theme	new	1120:1122	arg1	possibility					1124:1134	a new possibility	1118:1134	a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification	1118:1244	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	0	48	theme	friendly	53:60	arg1	membrane					90:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	4	49	theme	violet	722:727	arg1	removal					734:740	crystal violet (CV) removal	714:740	crystal violet (CV) removal by the CS/PG	714:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	6	50	theme	rejection	984:992	arg1	effect					974:979	the synergistic effect	958:979	the synergistic effect of rejection and adsorption	958:1007	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	0	51	theme	environmentally	37:51	arg1	membrane					90:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	2	52	theme	low-cost	245:252	arg1	PG					290:291	PG	290:291	PG	290:291	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	52	theme	low-cost	245:252	arg1	support					310:316	a support	308:316	a support	308:316	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	52	theme	low-cost	245:252	arg1	geopolymer					278:287	The low-cost and eco-friendly porous geopolymer	241:287	The low-cost and eco-friendly porous geopolymer (PG)	241:292	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	3	53	theme	1.0	437:439	arg1	%					440:440	%	440:440	%	440:440	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	4	54	theme	continuous	775:784	arg1	mode					786:789	a continuous mode	773:789	a continuous mode	773:789	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	3	55	theme	high	575:578	arg1	flux					586:589	a high water flux	573:589	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	56	theme	average	528:534	arg1	diameter					541:548	an average pore diameter	525:548	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	3	57	theme	%	518:518	arg1	porosity					501:508	a high porosity	494:508	a high porosity of 50.97%	494:518	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	4	58	from	effects	626:632	arg1	removal					734:740	crystal violet (CV) removal	714:740	crystal violet (CV) removal by the CS/PG	714:753	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	3	59	theme	pore	536:539	arg1	diameter					541:548	an average pore diameter	525:548	an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar	525:619	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	5	60	theme	%	817:817	arg1	CV					819:820	~95% CV	814:820	~95% CV	814:820	The results indicated ~95% CV could be removed from water during continuous treating of 14 h.
31989986	6	61	theme	synergistic	962:972	arg1	effect					974:979	the synergistic effect	958:979	the synergistic effect of rejection and adsorption	958:1007	The effectiveness in CV removal by the CS/PG membrane was attributed to the synergistic effect of rejection and adsorption.
31989986	8	62	from	applications	1211:1222	arg1	purification					1233:1244	water purification	1227:1244	water purification	1227:1244	Overall, this work opens a new possibility of building cost-saving and eco-friendly composite membranes for practical applications in water purification.
31989986	0	63	theme	composite	80:88	arg1	membrane					90:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	4	64	theme	feed	697:700	arg1	solution					702:709	the feed solution	693:709	the feed solution	693:709	The effects of initial concentration, pH, flow rate and temperature of the feed solution on crystal violet (CV) removal by the CS/PG were evaluated in a continuous mode.
31989986	2	65	theme	green	346:350	arg1	chitosan					328:335	chitosan	328:335	chitosan (CS)	328:340	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	2	65	theme	green	346:350	arg1	biomaterial					353:363	a "green" biomaterial	343:363	a "green" biomaterial	343:363	The low-cost and eco-friendly porous geopolymer (PG) was chosen as a support, on which chitosan (CS), a "green" biomaterial, was used to form an active layer.
31989986	0	66	theme	inorganic-organic	62:78	arg1	membrane					90:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	a low-cost and environmentally friendly inorganic-organic composite membrane	22:97	Facile fabrication of a low-cost and environmentally friendly inorganic-organic composite membrane for aquatic dye removal.
31989986	3	67	dep	CS	423:424	arg1	2.0 mL					427:432	2.0 mL	427:432	2.0 mL of 1.0% CS solution	427:452	With optimum dosage of CS (2.0 mL of 1.0% CS solution), the obtained CS/PG membrane exhibited a high porosity of 50.97% with an average pore diameter of 13.93 nm as well as a high water flux of 1663.82 ± 22.46 L/m2·h·bar.
31989986	1	68	theme	inorganic-organic	149:165	arg1	membrane					177:184	a new inorganic-organic composite membrane	143:184	a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method	143:238	This study reports a new inorganic-organic composite membrane fabricated by an electrostatic self-assembling method.
29792961	11	0	from	applications	1465:1476	arg1	industry					1490:1497	the food industry	1481:1497	the food industry	1481:1497	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	2	1	theme	water	339:343	arg1	adsorption					345:354	water adsorption	339:354	water adsorption	339:354	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	5	2	theme	cellulose-based	705:719	arg1	films					721:725	cellulose-based films	705:725	cellulose-based films	705:725	Results showed that chitosan and polyvinyl alcohol enhanced the mechanical properties of cellulose-based films.
29792961	1	3	theme	polyvinyl	226:234	arg1	alcohol					236:242	polyvinyl alcohol	226:242	polyvinyl alcohol	226:242	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	2	4	theme	contents	474:481	arg1	function					399:406	a function	397:406	a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	397:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	4	theme	contents	474:481	arg1	properties					356:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	6	5	theme	strength	783:790	arg1	values					792:797	burst strength values	777:797	burst strength values ranged from 929 to 6291 g	777:823	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	7	6	theme	films	1000:1004	arg1	effect					986:991	the UV light protection effect	962:991	the UV light protection effect of the films	962:1004	Cellulose and chitosan improved the UV light protection effect of the films.
29792961	0	7	theme	water	91:95	arg1	adsorption					97:106	water adsorption	91:106	water adsorption	91:106	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	10	8	with	films	1255:1259	arg1	properties					1286:1295	improved mechanical properties	1266:1295	improved mechanical properties	1266:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	6	9	theme	burst	777:781	arg1	values					792:797	burst strength values	777:797	burst strength values ranged from 929 to 6291 g	777:823	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	11	10	theme	food	1485:1488	arg1	industry					1490:1497	the food industry	1481:1497	the food industry	1481:1497	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	7	11	theme	UV	966:967	arg1	effect					986:991	the UV light protection effect	962:991	the UV light protection effect of the films	962:1004	Cellulose and chitosan improved the UV light protection effect of the films.
29792961	6	12	dep	2.52 mm	864:870	arg1	to					861:862	to	861:862	to	861:862	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	2	13	theme	alcohol	459:465	arg1	contents					474:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	4	14	theme	structural	569:578	arg1	properties					531:540	Light-barrier properties	517:540	Light-barrier properties	517:540	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	4	14	theme	structural	569:578	arg1	analyses					592:599	structural and thermal analyses	569:599	structural and thermal analyses	569:599	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	10	15	theme	composite	1245:1253	arg1	capacity					1303:1310	high capacity	1298:1310	high capacity to adsorb water	1298:1326	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	15	theme	composite	1245:1253	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	15	theme	composite	1245:1253	arg1	properties					1342:1351	good barrier properties	1329:1351	good barrier properties	1329:1351	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	0	16	theme	adsorption	97:106	arg1	Evaluation					77:86	Evaluation	77:86	Evaluation of water adsorption	77:106	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	4	17	theme	Light-barrier	517:529	arg1	properties					531:540	Light-barrier properties	517:540	Light-barrier properties	517:540	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	4	17	theme	Light-barrier	517:529	arg1	analyses					592:599	structural and thermal analyses	569:599	structural and thermal analyses	569:599	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	4	17	theme	Light-barrier	517:529	arg1	morphology					557:566	morphology	557:566	morphology	557:566	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	4	17	theme	Light-barrier	517:529	arg1	transparency					543:554	transparency	543:554	transparency	543:554	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	10	18	theme	transparency	1388:1399	arg1	value					1401:1405	adequate transparency value	1379:1405	adequate transparency value	1379:1405	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	8	19	dep	improved	1058:1065	arg1	increased					1020:1028	increased	1020:1028	increased	1020:1028	However, PVA increased the transmittance meanwhile improved the film transparency.
29792961	10	20	theme	alcohol	1237:1243	arg1	capacity					1303:1310	high capacity	1298:1310	high capacity to adsorb water	1298:1326	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	20	theme	alcohol	1237:1243	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	20	theme	alcohol	1237:1243	arg1	properties					1342:1351	good barrier properties	1329:1351	good barrier properties	1329:1351	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	1	21	theme	study	160:164	arg1	aim					148:150	The aim	144:150	The aim of this study	144:164	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	1	22	from	alcohol	236:242	arg1	films					191:195	composite films	181:195	composite films from cellulose, chitosan and polyvinyl alcohol	181:242	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	10	23	theme	adequate	1379:1386	arg1	value					1401:1405	adequate transparency value	1379:1405	adequate transparency value	1379:1405	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	2	24	theme	strength	307:314	arg1	function					399:406	a function	397:406	a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	397:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	24	theme	strength	307:314	arg1	properties					356:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	10	25	theme	cellulose-chitosan-polyvinyl	1208:1235	arg1	capacity					1303:1310	high capacity	1298:1310	high capacity to adsorb water	1298:1326	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	25	theme	cellulose-chitosan-polyvinyl	1208:1235	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	25	theme	cellulose-chitosan-polyvinyl	1208:1235	arg1	properties					1342:1351	good barrier properties	1329:1351	good barrier properties	1329:1351	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	0	26	theme	Composite	0:8	arg1	properties					132:141	mechanical and optical properties	109:141	mechanical and optical properties	109:141	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	0	26	theme	Composite	0:8	arg1	films					10:14	Composite films	0:14	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol	0:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	1	27	theme	environmentally	254:268	arg1	materials					279:287	environmentally friendly materials	254:287	environmentally friendly materials	254:287	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	9	28	theme	films	1156:1160	arg1	components					1138:1147	the components	1134:1147	the components of the films	1134:1160	FT-IR and DSC showed an interaction between the components of the films.
29792961	2	29	theme	burst	301:305	arg1	strength					307:314	burst strength	301:314	burst strength	301:314	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	0	30	theme	regenerate	19:28	arg1	cellulose					30:38	regenerate cellulose	19:38	regenerate cellulose	19:38	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	1	31	theme	friendly	270:277	arg1	materials					279:287	environmentally friendly materials	254:287	environmentally friendly materials	254:287	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	5	32	theme	polyvinyl	649:657	arg1	alcohol					659:665	polyvinyl alcohol	649:665	polyvinyl alcohol	649:665	Results showed that chitosan and polyvinyl alcohol enhanced the mechanical properties of cellulose-based films.
29792961	10	33	theme	barrier	1334:1340	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	33	theme	barrier	1334:1340	arg1	properties					1342:1351	good barrier properties	1329:1351	good barrier properties	1329:1351	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	2	34	dep	burst	329:333	arg1	to					326:327	to	326:327	to	326:327	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	6	35	dep	8.01 MJ/m3	765:774	arg1	to					762:763	to	762:763	to	762:763	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	2	36	theme	cellulose	411:419	arg1	contents					474:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	0	37	theme	mechanical	109:118	arg1	properties					132:141	mechanical and optical properties	109:141	mechanical and optical properties	109:141	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	0	37	theme	mechanical	109:118	arg1	films					10:14	Composite films	0:14	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol	0:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	10	38	theme	UV	1361:1362	arg1	radiations					1364:1373	UV radiations	1361:1373	UV radiations	1361:1373	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	2	39	theme	Toughness	290:298	arg1	function					399:406	a function	397:406	a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	397:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	39	theme	Toughness	290:298	arg1	properties					356:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	10	40	theme	improved	1266:1273	arg1	properties					1286:1295	improved mechanical properties	1266:1295	improved mechanical properties	1266:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	1	41	theme	composite	181:189	arg1	films					191:195	composite films	181:195	composite films from cellulose, chitosan and polyvinyl alcohol	181:242	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	10	42	theme	high	1298:1301	arg1	capacity					1303:1310	high capacity	1298:1310	high capacity to adsorb water	1298:1326	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	42	theme	high	1298:1301	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	11	43	theme	partial	1507:1513	arg1	alternative					1515:1525	a partial alternative	1505:1525	a partial alternative to synthetic films	1505:1544	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	2	44	theme	chitosan	429:436	arg1	contents					474:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	411:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	6	45	dep	6291 g	818:823	arg1	to					815:816	to	815:816	to	815:816	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	0	46	theme	cellulose	30:38	arg1	properties					132:141	mechanical and optical properties	109:141	mechanical and optical properties	109:141	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	0	46	theme	cellulose	30:38	arg1	films					10:14	Composite films	0:14	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol	0:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	2	47	theme	polyvinyl	449:457	arg1	alcohol					459:465	polyvinyl alcohol	449:465	polyvinyl alcohol (0-4%)	449:472	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	47	theme	polyvinyl	449:457	arg1	%					471:471	0-4%	468:471	0-4%	468:471	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	5	48	theme	mechanical	680:689	arg1	properties					691:700	the mechanical properties	676:700	the mechanical properties of cellulose-based films	676:725	Results showed that chitosan and polyvinyl alcohol enhanced the mechanical properties of cellulose-based films.
29792961	1	49	from	chitosan	213:220	arg1	films					191:195	composite films	181:195	composite films from cellulose, chitosan and polyvinyl alcohol	181:242	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	8	50	theme	film	1071:1074	arg1	transparency					1076:1087	the film transparency	1067:1087	the film transparency	1067:1087	However, PVA increased the transmittance meanwhile improved the film transparency.
29792961	6	51	theme	Toughness	728:736	arg1	values					738:743	Toughness values	728:743	Toughness values ranged from 0.47 to 8.01 MJ/m3	728:774	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	2	52	theme	burst	329:333	arg1	function					399:406	a function	397:406	a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	397:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	52	theme	burst	329:333	arg1	properties					356:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	0	53	theme	optical	124:130	arg1	properties					132:141	mechanical and optical properties	109:141	mechanical and optical properties	109:141	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	0	53	theme	optical	124:130	arg1	films					10:14	Composite films	0:14	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol	0:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	6	54	theme	adsorption	882:891	arg1	values					893:898	water adsorption values	876:898	water adsorption values	876:898	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	8	55	theme	transmittance	1034:1046	arg1	meanwhile					1048:1056	the transmittance meanwhile	1030:1056	the transmittance meanwhile	1030:1056	However, PVA increased the transmittance meanwhile improved the film transparency.
29792961	7	56	theme	light	969:973	arg1	effect					986:991	the UV light protection effect	962:991	the UV light protection effect of the films	962:1004	Cellulose and chitosan improved the UV light protection effect of the films.
29792961	0	57	with	films	10:14	arg1	chitosan					45:52	chitosan	45:52	chitosan	45:52	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	0	57	with	films	10:14	arg1	alcohol					68:74	polyvinyl alcohol	58:74	polyvinyl alcohol	58:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	6	58	theme	water	876:880	arg1	values					893:898	water adsorption values	876:898	water adsorption values	876:898	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	1	59	from	cellulose	202:210	arg1	films					191:195	composite films	181:195	composite films from cellulose, chitosan and polyvinyl alcohol	181:242	The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials.
29792961	2	60	theme	distance	317:324	arg1	burst					329:333	distance to burst	317:333	distance to burst	317:333	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	3	61	theme	Polynomial	484:493	arg1	models					495:500	Polynomial models	484:500	Polynomial models	484:500	Polynomial models were obtained.
29792961	7	62	theme	protection	975:984	arg1	effect					986:991	the UV light protection effect	962:991	the UV light protection effect of the films	962:1004	Cellulose and chitosan improved the UV light protection effect of the films.
29792961	6	63	dep	143.56	921:926	arg1	to					918:919	to	918:919	to	918:919	Toughness values ranged from 0.47 to 8.01 MJ/m3, burst strength values ranged from 929 to 6291 g, distance to burst ranged from 1.25 to 2.52 mm and water adsorption values ranged from 52.30 to 143.56%.
29792961	10	64	theme	good	1329:1332	arg1	films					1255:1259	cellulose-chitosan-polyvinyl alcohol composite films	1208:1259	cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties	1208:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	64	theme	good	1329:1332	arg1	properties					1342:1351	good barrier properties	1329:1351	good barrier properties	1329:1351	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	10	65	theme	mechanical	1275:1284	arg1	properties					1286:1295	improved mechanical properties	1266:1295	improved mechanical properties	1266:1295	Results showed that it is feasible to obtain cellulose-chitosan-polyvinyl alcohol composite films with improved mechanical properties, high capacity to adsorb water, good barrier properties against UV radiations and adequate transparency value.
29792961	11	66	theme	potential	1445:1453	arg1	applications					1465:1476	potential packaging applications	1445:1476	potential packaging applications in the food industry	1445:1497	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	5	67	theme	films	721:725	arg1	properties					691:700	the mechanical properties	676:700	the mechanical properties of cellulose-based films	676:725	Results showed that chitosan and polyvinyl alcohol enhanced the mechanical properties of cellulose-based films.
29792961	4	68	theme	thermal	584:590	arg1	properties					531:540	Light-barrier properties	517:540	Light-barrier properties	517:540	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	4	68	theme	thermal	584:590	arg1	analyses					592:599	structural and thermal analyses	569:599	structural and thermal analyses	569:599	Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed.
29792961	0	69	dep	films	10:14	arg1	Evaluation					77:86	Evaluation	77:86	Evaluation of water adsorption	77:106	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	11	70	theme	packaging	1455:1463	arg1	applications					1465:1476	potential packaging applications	1445:1476	potential packaging applications in the food industry	1445:1497	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	0	71	theme	polyvinyl	58:66	arg1	alcohol					68:74	polyvinyl alcohol	58:74	polyvinyl alcohol	58:74	Composite films of regenerate cellulose with chitosan and polyvinyl alcohol: Evaluation of water adsorption, mechanical and optical properties.
29792961	11	72	theme	synthetic	1530:1538	arg1	films					1540:1544	synthetic films	1530:1544	synthetic films	1530:1544	These properties could be useful for potential packaging applications in the food industry or as a partial alternative to synthetic films.
29792961	2	73	theme	adsorption	345:354	arg1	function					399:406	a function	397:406	a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents	397:481	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
29792961	2	73	theme	adsorption	345:354	arg1	properties					356:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties	290:365	Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents.
31374278	7	0	theme	starch/PVA	1198:1207	arg1	matrix					1209:1214	the biodegradable starch/PVA matrix	1180:1214	the biodegradable starch/PVA matrix	1180:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	3	1	theme	microscopy	695:704	arg1	methods					752:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	1	2	theme	eco-friendly	249:260	arg1	matrix					273:278	a mixed eco-friendly starch/PVA matrix	241:278	a mixed eco-friendly starch/PVA matrix	241:278	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	6	3	theme	TiO2	1126:1129	arg1	concentration					1131:1143	the TiO2 concentration	1122:1143	the TiO2 concentration	1122:1143	The UV- protective ability of the composite films rose as the TiO2 concentration increased.
31374278	3	4	theme	TiO2	578:581	arg1	content					590:596	the TiO2 filler content	574:596	the TiO2 filler content	574:596	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	6	5	theme	films	1108:1112	arg1	ability					1083:1089	The UV- protective ability	1064:1089	The UV- protective ability of the composite films	1064:1112	The UV- protective ability of the composite films rose as the TiO2 concentration increased.
31374278	3	6	theme	spectroscopy	739:750	arg1	methods					752:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	0	7	theme	biodegradable	100:112	arg1	films					141:145	biodegradable maize starch/PVA composite films	100:145	biodegradable maize starch/PVA composite films	100:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	5	8	theme	TiO2	1032:1035	arg1	concentration					1037:1049	the TiO2 concentration	1028:1049	the TiO2 concentration of 0.05 wt%	1028:1061	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	0	9	theme	starch/PVA	120:129	arg1	films					141:145	biodegradable maize starch/PVA composite films	100:145	biodegradable maize starch/PVA composite films	100:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	1	10	theme	"	199:199	arg1	materials					222:230	"green" multifunctional film materials	193:230	"green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	193:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	7	11	attach	presence	1160:1167	arg2	TiO2					1172:1175	TiO2	1172:1175	TiO2	1172:1175	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	7	11	attach	presence	1160:1167	arg1	matrix					1209:1214	the biodegradable starch/PVA matrix	1180:1214	the biodegradable starch/PVA matrix	1180:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	0	12	theme	films	141:145	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	3	13	theme	content	590:596	arg1	influence					561:569	The influence	557:569	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites	557:666	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	4	14	theme	polymer	894:900	arg1	weight					902:907	the dry polymer weight	886:907	the dry polymer weight	886:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	8	15	theme	starch/PVA	1415:1424	arg1	properties					1432:1441	starch/PVA films properties	1415:1441	starch/PVA films properties	1415:1441	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	7	16	theme	biodegradable	1184:1196	arg1	matrix					1209:1214	the biodegradable starch/PVA matrix	1180:1214	the biodegradable starch/PVA matrix	1180:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	0	17	from	Effect	0:5	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	7	18	theme	TiO2	1172:1175	arg1	presence					1160:1167	The presence	1156:1167	The presence of TiO2 in the biodegradable starch/PVA matrix	1156:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	5	19	theme	%	1061:1061	arg1	concentration					1037:1049	the TiO2 concentration	1028:1049	the TiO2 concentration of 0.05 wt%	1028:1061	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	5	20	theme	water	950:954	arg1	permeability					962:973	water vapor permeability	950:973	water vapor permeability	950:973	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	4	21	theme	polymer	786:792	arg1	components					794:803	the polymer components	782:803	the polymer components	782:803	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	5	22	from	concentration	1037:1049	arg1	maximum					1017:1023	a maximum	1015:1023	a maximum at the TiO2 concentration of 0.05 wt%	1015:1061	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	5	23	theme	permeability	962:973	arg1	dependence					915:924	The dependence	911:924	The dependence of tensile strength and water vapor permeability on the filler content	911:995	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	2	24	from	time	489:492	arg1	components					466:475	the components	462:475	the components at the same time	462:492	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	4	25	dep	filler	857:862	arg1	weight					902:907	the dry polymer weight	886:907	the dry polymer weight	886:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	4	25	dep	filler	857:862	arg1	%					881:881	0.01 and 0.05 wt%	865:881	0.01 and 0.05 wt% of the dry polymer weight	865:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	1	26	theme	starch/PVA	262:271	arg1	matrix					273:278	a mixed eco-friendly starch/PVA matrix	241:278	a mixed eco-friendly starch/PVA matrix	241:278	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	7	27	theme	composite	1280:1288	arg1	films					1290:1294	the composite films	1276:1294	the composite films	1276:1294	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	8	28	theme	fibrous	1334:1340	arg1	particles					1347:1355	fibrous TiO2 particles	1334:1355	fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt%	1334:1401	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	0	29	theme	fibrous	10:16	arg1	filler					23:28	fibrous TiO2 filler	10:28	fibrous TiO2 filler	10:28	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	5	30	theme	strength	937:944	arg1	dependence					915:924	The dependence	911:924	The dependence of tensile strength and water vapor permeability on the filler content	911:995	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	0	31	theme	filler	23:28	arg1	Effect					0:5	Effect	0:5	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.	0:146	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	7	32	theme	biodegradation	1253:1266	arg1	rate					1268:1271	the soil burial biodegradation rate	1237:1271	the soil burial biodegradation rate of the composite films	1237:1294	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	2	33	theme	same	484:487	arg1	time					489:492	the same time	480:492	the same time	480:492	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	8	34	theme	optimum	1360:1366	arg1	concentration					1368:1380	optimum concentration	1360:1380	optimum concentration of 0.01 and 0.05 wt%	1360:1401	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	1	35	theme	TiO2	304:307	arg1	particles					309:317	fibrous TiO2 particles	296:317	fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	296:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	7	36	theme	soil	1241:1244	arg1	rate					1268:1271	the soil burial biodegradation rate	1237:1271	the soil burial biodegradation rate of the composite films	1237:1294	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	5	37	contain	have	1010:1013	arg1	dependence					915:924	The dependence	911:924	The dependence of tensile strength and water vapor permeability on the filler content	911:995	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	5	37	contain	have	1010:1013	arg2	maximum					1017:1023	a maximum	1015:1023	a maximum at the TiO2 concentration of 0.05 wt%	1015:1061	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	3	38	theme	optical	687:693	arg1	microscopy					695:704	optical microscopy	687:704	optical microscopy	687:704	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	1	39	theme	multifunctional	201:215	arg1	materials					222:230	"green" multifunctional film materials	193:230	"green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	193:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	6	40	theme	protective	1072:1081	arg1	ability					1083:1089	The UV- protective ability	1064:1089	The UV- protective ability of the composite films	1064:1112	The UV- protective ability of the composite films rose as the TiO2 concentration increased.
31374278	2	41	theme	Starch/PVA/TiO2	373:387	arg1	dispersions					402:412	Starch/PVA/TiO2 film-forming dispersions	373:412	Starch/PVA/TiO2 film-forming dispersions	373:412	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	7	42	theme	little	1220:1225	arg1	effect					1227:1232	little effect	1220:1232	little effect	1220:1232	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	3	43	theme	diffraction	713:723	arg1	methods					752:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	1	44	theme	mixed	243:247	arg1	matrix					273:278	a mixed eco-friendly starch/PVA matrix	241:278	a mixed eco-friendly starch/PVA matrix	241:278	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	3	45	theme	FTIR	734:737	arg1	spectroscopy					739:750	FTIR spectroscopy	734:750	FTIR spectroscopy	734:750	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	3	46	theme	DSC	726:728	arg1	methods					752:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods	683:758	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	4	47	theme	filler	857:862	arg1	filler					857:862	the filler	853:862	the filler (0.01 and 0.05 wt% of the dry polymer weight)	853:908	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	4	47	theme	filler	857:862	arg1	amount					843:848	a small amount	835:848	a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight)	835:908	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	0	48	theme	maize	114:118	arg1	films					141:145	biodegradable maize starch/PVA composite films	100:145	biodegradable maize starch/PVA composite films	100:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	4	49	theme	weight	902:907	arg1	weight					902:907	the dry polymer weight	886:907	the dry polymer weight	886:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	4	49	theme	weight	902:907	arg1	%					881:881	0.01 and 0.05 wt%	865:881	0.01 and 0.05 wt% of the dry polymer weight	865:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	1	50	dep	materials	222:230	arg1	filled					284:289	filled	284:289	filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	284:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	1	50	dep	materials	222:230	arg1	based					232:236	based	232:236	based on a mixed eco-friendly starch/PVA matrix	232:278	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	6	51	theme	composite	1098:1106	arg1	films					1108:1112	the composite films	1094:1112	the composite films	1094:1112	The UV- protective ability of the composite films rose as the TiO2 concentration increased.
31374278	0	52	theme	composite	131:139	arg1	films					141:145	biodegradable maize starch/PVA composite films	100:145	biodegradable maize starch/PVA composite films	100:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	8	53	from	concentration	1368:1380	arg1	particles					1347:1355	fibrous TiO2 particles	1334:1355	fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt%	1334:1401	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	1	54	theme	green	194:198	arg1	materials					222:230	"green" multifunctional film materials	193:230	"green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	193:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	2	55	theme	casting	541:547	arg1	method					549:554	the casting method	537:554	the casting method	537:554	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	7	56	from	presence	1160:1167	arg1	matrix					1209:1214	the biodegradable starch/PVA matrix	1180:1214	the biodegradable starch/PVA matrix	1180:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	3	57	theme	filler	583:588	arg1	content					590:596	the TiO2 filler content	574:596	the TiO2 filler content	574:596	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	1	58	theme	ultrasound-assisted	333:351	arg1	method					365:370	the ultrasound-assisted biotemplate method	329:370	the ultrasound-assisted biotemplate method	329:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	8	59	theme	films	1426:1430	arg1	properties					1432:1441	starch/PVA films properties	1415:1441	starch/PVA films properties	1415:1441	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	3	60	from	influence	561:569	arg1	characteristics					634:648	the morphological and structural characteristics	601:648	the morphological and structural characteristics of the composites	601:666	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	3	61	theme	structural	623:632	arg1	characteristics					634:648	the morphological and structural characteristics	601:648	the morphological and structural characteristics of the composites	601:666	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	4	62	theme	dry	890:892	arg1	weight					902:907	the dry polymer weight	886:907	the dry polymer weight	886:907	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	0	63	theme	mechanical	49:58	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	3	64	theme	morphological	605:617	arg1	characteristics					634:648	the morphological and structural characteristics	601:648	the morphological and structural characteristics of the composites	601:666	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	2	65	theme	composite	504:512	arg1	films					514:518	then composite films	499:518	then composite films	499:518	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	0	66	theme	barrier	61:67	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	1	67	theme	biotemplate	353:363	arg1	method					365:370	the ultrasound-assisted biotemplate method	329:370	the ultrasound-assisted biotemplate method	329:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	8	68	theme	%	1401:1401	arg1	concentration					1368:1380	optimum concentration	1360:1380	optimum concentration of 0.01 and 0.05 wt%	1360:1401	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	0	69	theme	optical	73:79	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	7	70	contain	had	1216:1218	arg1	presence					1160:1167	The presence	1156:1167	The presence of TiO2 in the biodegradable starch/PVA matrix	1156:1214	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	7	70	contain	had	1216:1218	arg2	effect					1227:1232	little effect	1220:1232	little effect	1220:1232	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	5	71	theme	vapor	956:960	arg1	permeability					962:973	water vapor permeability	950:973	water vapor permeability	950:973	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	7	72	theme	films	1290:1294	arg1	rate					1268:1271	the soil burial biodegradation rate	1237:1271	the soil burial biodegradation rate of the composite films	1237:1294	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	2	73	theme	film-forming	389:400	arg1	dispersions					402:412	Starch/PVA/TiO2 film-forming dispersions	373:412	Starch/PVA/TiO2 film-forming dispersions	373:412	Starch/PVA/TiO2 film-forming dispersions were prepared by heating and intensively mixing the components at the same time, and then composite films were obtained by the casting method.
31374278	0	74	theme	TiO2	18:21	arg1	filler					23:28	fibrous TiO2 filler	10:28	fibrous TiO2 filler	10:28	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	5	75	theme	tensile	929:935	arg1	strength					937:944	tensile strength	929:944	tensile strength	929:944	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	8	76	theme	obtained	1307:1314	arg1	results					1316:1322	the obtained results	1303:1322	the obtained results	1303:1322	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	3	77	theme	composites	657:666	arg1	characteristics					634:648	the morphological and structural characteristics	601:648	the morphological and structural characteristics of the composites	601:666	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
31374278	7	78	theme	burial	1246:1251	arg1	rate					1268:1271	the soil burial biodegradation rate	1237:1271	the soil burial biodegradation rate of the composite films	1237:1294	The presence of TiO2 in the biodegradable starch/PVA matrix had little effect on the soil burial biodegradation rate of the composite films.
31374278	8	79	theme	TiO2	1342:1345	arg1	particles					1347:1355	fibrous TiO2 particles	1334:1355	fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt%	1334:1401	Thus, the obtained results show that fibrous TiO2 particles at optimum concentration of 0.01 and 0.05 wt% can improve starch/PVA films properties and extend their functionality.
31374278	0	80	theme	structural	37:46	arg1	characteristics					81:95	the structural, mechanical, barrier and optical characteristics	33:95	the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films	33:145	Effect of fibrous TiO2 filler on the structural, mechanical, barrier and optical characteristics of biodegradable maize starch/PVA composite films.
31374278	1	81	theme	fibrous	296:302	arg1	particles					309:317	fibrous TiO2 particles	296:317	fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	296:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	4	82	theme	small	837:841	arg1	filler					857:862	the filler	853:862	the filler (0.01 and 0.05 wt% of the dry polymer weight)	853:908	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	4	82	theme	small	837:841	arg1	amount					843:848	a small amount	835:848	a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight)	835:908	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	4	83	theme	components	794:803	arg1	compatibility					765:777	The compatibility	761:777	The compatibility of the polymer components	761:803	The compatibility of the polymer components was improved by incorporating a small amount of the filler (0.01 and 0.05 wt% of the dry polymer weight).
31374278	5	84	from	dependence	915:924	arg1	content					989:995	the filler content	978:995	the filler content	978:995	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	1	85	theme	film	217:220	arg1	materials					222:230	"green" multifunctional film materials	193:230	"green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method	193:370	The work focuses on producing and evaluating "green" multifunctional film materials based on a mixed eco-friendly starch/PVA matrix and filled with fibrous TiO2 particles formed by the ultrasound-assisted biotemplate method.
31374278	5	86	theme	filler	982:987	arg1	content					989:995	the filler content	978:995	the filler content	978:995	The dependence of tensile strength and water vapor permeability on the filler content was shown to have a maximum at the TiO2 concentration of 0.05 wt%.
31374278	6	87	theme	UV-	1068:1070	arg1	ability					1083:1089	The UV- protective ability	1064:1089	The UV- protective ability of the composite films	1064:1112	The UV- protective ability of the composite films rose as the TiO2 concentration increased.
31374278	3	88	theme	X-ray	707:711	arg1	diffraction					713:723	X-ray diffraction	707:723	X-ray diffraction	707:723	The influence of the TiO2 filler content on the morphological and structural characteristics of the composites was studied by the optical microscopy, X-ray diffraction, DSC and FTIR spectroscopy methods.
30913409	2	0	with	mixture	419:425	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	6	1	located	observed	1152:1159	arg1	emulsions					1171:1179	cooked emulsions	1164:1179	cooked emulsions	1164:1179	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	6	1	located	observed	1152:1159	arg2	differences					1131:1141	these differences	1125:1141	these differences	1125:1141	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	1	2	theme	sourced	152:158	arg1	βG					173:174	βG	173:174	βG	173:174	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	2	theme	sourced	152:158	arg1	beta-glucan					160:170	Barley sourced beta-glucan	145:170	Barley sourced beta-glucan (βG)	145:175	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	2	3	theme	MCC	368:370	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	3	theme	MCC	368:370	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	6	4	dep	greater	1019:1025	arg1	P < .05					1028:1034	P < .05	1028:1034	P < .05	1028:1034	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	2	5	theme	%	388:388	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	6	6	theme	greater	1019:1025	arg1	values					1040:1045	greater (P < .05) b* values	1019:1045	greater (P < .05) b* values	1019:1045	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	2	7	theme	MCC	444:446	arg1	mixture					419:425	a mixture	417:425	a mixture of βG (1.5%) and MCC (1.5%)	417:453	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	7	theme	MCC	444:446	arg1	Emulsions					297:305	Emulsions	297:305	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	297:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	8	theme	independent	575:585	arg1	replications					587:598	three independent replications	569:598	three independent replications	569:598	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	6	9	contain	had	1015:1017	arg1	Emulsions					988:996	Emulsions	988:996	Emulsions prepared with βG	988:1013	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	6	9	contain	had	1015:1017	arg2	values					1040:1045	greater (P < .05) b* values	1019:1045	greater (P < .05) b* values	1019:1045	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	1	10	theme	beef	236:239	arg1	emulsions					241:249	beef emulsions	236:249	beef emulsions containing beef, olive oil, salts, and water	236:294	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	6	11	theme	*	1038:1038	arg1	values					1040:1045	greater (P < .05) b* values	1019:1045	greater (P < .05) b* values	1019:1045	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	1	12	contain	containing	251:260	arg2	salts					279:283	salts	279:283	salts	279:283	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	12	contain	containing	251:260	arg1	emulsions					241:249	beef emulsions	236:249	beef emulsions containing beef, olive oil, salts, and water	236:294	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	12	contain	containing	251:260	arg2	oil					274:276	olive oil	268:276	olive oil	268:276	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	12	contain	containing	251:260	arg2	water					290:294	water	290:294	water	290:294	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	5	13	dep	lower	794:798	arg1	P < .05					801:807	P < .05	801:807	P < .05	801:807	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	3	14	dep	differed	636:643	arg1	based					645:649	based	645:649	differed based mainly on the hydrocolloid used and formulation	636:697	As expected, proximate composition differed based mainly on the hydrocolloid used and formulation.
30913409	2	15	theme	%	333:333	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	16	theme	βG	364:365	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	17	theme	starch	376:381	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	18	theme	instrumental	511:522	arg1	color					524:528	instrumental color	511:528	instrumental color	511:528	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	19	from	composition	484:494	arg1	replications					587:598	three independent replications	569:598	three independent replications	569:598	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	20	theme	βG	393:394	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	21	theme	βG	430:431	arg1	mixture					419:425	a mixture	417:425	a mixture of βG (1.5%) and MCC (1.5%)	417:453	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	21	theme	βG	430:431	arg1	Emulsions					297:305	Emulsions	297:305	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	297:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	0	22	theme	beef	20:23	arg1	models					34:39	beef emulsion models	20:39	beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose	20:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	2	23	theme	MCC	397:399	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	23	theme	MCC	397:399	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	1	24	theme	microcrystalline	178:193	arg1	cellulose					195:203	microcrystalline cellulose	178:203	microcrystalline cellulose (MCC)	178:209	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	24	theme	microcrystalline	178:193	arg1	MCC					206:208	MCC	206:208	MCC	206:208	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	6	25	theme	cooked	1164:1169	arg1	emulsions					1171:1179	cooked emulsions	1164:1179	cooked emulsions	1164:1179	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	1	26	theme	beef	262:265	arg1	oil					274:276	olive oil	268:276	olive oil	268:276	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	0	27	theme	β-glucan	104:111	arg1	cellulose					73:81	microcrystalline cellulose	56:81	microcrystalline cellulose	56:81	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	27	theme	β-glucan	104:111	arg1	combination					89:99	a combination	87:99	a combination of β-glucan and microcrystalline cellulose	87:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	27	theme	β-glucan	104:111	arg1	β-glucan					46:53	β-glucan	46:53	β-glucan	46:53	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	4	28	theme	Cooking	700:706	arg1	loss					708:711	Cooking loss	700:711	Cooking loss	700:711	Cooking loss was not different among treatments.
30913409	2	29	theme	profile	543:549	arg1	analyses					551:558	texture profile analyses	535:558	texture profile analyses	535:558	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	30	theme	MCC	342:344	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	0	31	theme	cellulose	134:142	arg1	cellulose					73:81	microcrystalline cellulose	56:81	microcrystalline cellulose	56:81	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	31	theme	cellulose	134:142	arg1	combination					89:99	a combination	87:99	a combination of β-glucan and microcrystalline cellulose	87:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	31	theme	cellulose	134:142	arg1	β-glucan					46:53	β-glucan	46:53	β-glucan	46:53	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	2	32	from	color	524:528	arg1	replications					587:598	three independent replications	569:598	three independent replications	569:598	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	33	theme	texture	535:541	arg1	analyses					551:558	texture profile analyses	535:558	texture profile analyses	535:558	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	0	34	theme	emulsion	25:32	arg1	models					34:39	beef emulsion models	20:39	beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose	20:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	1	35	theme	olive	268:272	arg1	oil					274:276	olive oil	268:276	olive oil	268:276	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	0	36	theme	microcrystalline	117:132	arg1	cellulose					134:142	microcrystalline cellulose	117:142	microcrystalline cellulose	117:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	2	37	theme	starch	350:355	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	37	theme	starch	350:355	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	38	theme	βG	338:339	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	5	39	theme	MCC	898:900	arg1	samples					913:919	MCC and starch samples	898:919	samples	913:919	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	5	40	theme	lower	794:798	arg1	values					810:815	lower (P < .05) values	794:815	lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness	794:882	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	2	41	theme	proximate	474:482	arg1	TPA					561:563	TPA	561:563	TPA	561:563	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	41	theme	proximate	474:482	arg1	composition					484:494	proximate composition	474:494	proximate composition	474:494	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	42	theme	%	359:359	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	5	43	theme	starch	906:911	arg1	samples					913:919	MCC and starch samples	898:919	samples	913:919	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	2	44	theme	starch	405:410	arg1	%					333:333	1%	332:333	1% of βG, MCC, or starch	332:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	starch					350:355	starch	350:355	starch	350:355	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	%					388:388	3%	387:388	3%	387:388	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	MCC					368:370	MCC	368:370	MCC	368:370	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	starch					405:410	starch	405:410	starch	405:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	βG					338:339	βG	338:339	βG	338:339	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	starch					376:381	starch	376:381	starch	376:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	MCC					342:344	MCC	342:344	MCC	342:344	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	βG					393:394	βG	393:394	βG	393:394	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	%					359:359	2%	358:359	2% of βG, MCC, or starch	358:381	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	MCC					397:399	MCC	397:399	MCC	397:399	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	44	theme	starch	405:410	arg1	βG					364:365	βG	364:365	βG	364:365	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	3	45	theme	proximate	614:622	arg1	composition					624:634	proximate composition	614:634	proximate composition	614:634	As expected, proximate composition differed based mainly on the hydrocolloid used and formulation.
30913409	5	46	theme	βG	776:777	arg1	samples					779:785	βG samples	776:785	βG samples	776:785	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	6	47	theme	b	1037:1037	arg1	values					1040:1045	greater (P < .05) b* values	1019:1045	greater (P < .05) b* values	1019:1045	Emulsions prepared with βG had greater (P < .05) b* values before cooking when compared with emulsions prepared with MCC and starch, but these differences were not observed in cooked emulsions.
30913409	2	48	with	Emulsions	297:305	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	0	49	with	models	34:39	arg1	cellulose					73:81	microcrystalline cellulose	56:81	microcrystalline cellulose	56:81	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	49	with	models	34:39	arg1	combination					89:99	a combination	87:99	a combination of β-glucan and microcrystalline cellulose	87:142	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	0	49	with	models	34:39	arg1	β-glucan					46:53	β-glucan	46:53	β-glucan	46:53	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	1	50	theme	Barley	145:150	arg1	βG					173:174	βG	173:174	βG	173:174	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	1	50	theme	Barley	145:150	arg1	beta-glucan					160:170	Barley sourced beta-glucan	145:170	Barley sourced beta-glucan (βG)	145:175	Barley sourced beta-glucan (βG), microcrystalline cellulose (MCC), or starch were added to beef emulsions containing beef, olive oil, salts, and water.
30913409	5	51	theme	similar	975:981	arg1	TPA					983:985	similar TPA	975:985	similar TPA	975:985	However, TPA differed with βG samples having lower (P < .05) values for hardness, adhesiveness, cohesiveness, gumminess, and chewiness compared with MCC and starch samples, while samples prepared with MCC and starch presented similar TPA.
30913409	0	52	theme	microcrystalline	56:71	arg1	cellulose					73:81	microcrystalline cellulose	56:81	microcrystalline cellulose	56:81	Replacing starch in beef emulsion models with β-glucan, microcrystalline cellulose, or a combination of β-glucan and microcrystalline cellulose.
30913409	2	53	from	loss	505:508	arg1	replications					587:598	three independent replications	569:598	three independent replications	569:598	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	54	from	analyses	551:558	arg1	replications					587:598	three independent replications	569:598	three independent replications	569:598	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	55	theme	cooking	497:503	arg1	loss					505:508	cooking loss	497:508	cooking loss	497:508	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30913409	2	56	theme	inclusion	312:320	arg1	levels					322:327	inclusion levels	312:327	inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch	312:410	Emulsions with inclusion levels of 1% of βG, MCC, or starch, 2% of βG, MCC, or starch, or 3% of βG, MCC, or starch, and a mixture of βG (1.5%) and MCC (1.5%) were evaluated for proximate composition, cooking loss, instrumental color, and texture profile analyses (TPA) in three independent replications.
30405163	0	0	theme	cellulose	80:88	arg1	CNC					104:106	CNC	104:106	CNC	104:106	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	0	0	theme	cellulose	80:88	arg1	nanocrystals					90:101	cellulose nanocrystals	80:101	cellulose nanocrystals (CNC)	80:107	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	4	1	theme	simultaneous	587:598	arg1	crystallization					600:614	simultaneous crystallization	587:614	simultaneous crystallization of cellulose	587:627	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	7	2	attach	presents	884:891	arg1	4 °C.					955:959	4 °C.	955:959	4 °C.	955:959	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	7	2	attach	presents	884:891	arg2	suspension					873:882	The resultant CNC suspension	855:882	The resultant CNC suspension	855:882	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	6	3	theme	CNC	741:743	arg1	properties					711:720	The morphology, spectroscopic, and stability properties	666:720	The morphology, spectroscopic, and stability properties of the as-prepared CNC	666:743	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	7	4	theme	resultant	859:867	arg1	suspension					873:882	The resultant CNC suspension	855:882	The resultant CNC suspension	855:882	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	1	5	theme	tough	233:237	arg1	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	5	theme	tough	233:237	arg1	nano-composites					239:253	tough nano-composites	233:253	tough nano-composites	233:253	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	4	6	theme	organosolv	515:524	arg1	solubilization					526:539	organosolv solubilization	515:539	organosolv solubilization	515:539	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	7	7	theme	CNC	971:973	arg1	phase					990:994	CNC liquid crystal phase	971:994	CNC liquid crystal phase	971:994	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	3	8	theme	inherent	396:403	arg1	limitations					405:415	inherent limitations	396:415	inherent limitations of corrosion hazards, time-consuming process, and/or low yield	396:478	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	0	9	theme	nanocrystals	90:101	arg1	isolation					67:75	high yield isolation	56:75	high yield isolation of cellulose nanocrystals (CNC)	56:107	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	6	10	theme	as-prepared	729:739	arg1	CNC					741:743	the as-prepared CNC	725:743	the as-prepared CNC	725:743	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	1	11	theme	due	255:257	arg1	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	11	theme	due	255:257	arg1	materials					200:208	responsive materials	189:208	responsive materials	189:208	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	7	12	with	property	900:907	arg1	stability					919:927	high stability	914:927	high stability	914:927	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	7	13	theme	aqueous	1075:1081	arg1	dispersion					1083:1092	the CNC aqueous dispersion	1067:1092	the CNC aqueous dispersion without stirring	1067:1109	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	8	14	theme	innovative	1116:1125	arg1	approach					1127:1134	The innovative approach	1112:1134	The innovative approach in this work	1112:1147	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	4	15	with	degradation	570:580	arg1	crystallization					600:614	simultaneous crystallization	587:614	simultaneous crystallization of cellulose	587:627	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	8	16	theme	organosolv	1251:1260	arg1	solubilization					1262:1275	organosolv solubilization	1251:1275	organosolv solubilization	1251:1275	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	3	17	theme	time-consuming	439:452	arg1	process					454:460	time-consuming process	439:460	time-consuming process	439:460	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	4	18	theme	cellulose	619:627	arg1	crystallization					600:614	simultaneous crystallization	587:614	simultaneous crystallization of cellulose	587:627	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	4	19	theme	irradiation	489:499	arg1	oxidation					501:509	irradiation oxidation	489:509	irradiation oxidation	489:509	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	1	20	theme	chiral	211:216	arg1	templates					218:226	chiral templates	211:226	chiral templates	211:226	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	20	theme	chiral	211:216	arg1	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	3	21	theme	process	454:460	arg1	limitations					405:415	inherent limitations	396:415	inherent limitations of corrosion hazards, time-consuming process, and/or low yield	396:478	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	0	22	theme	energy	5:10	arg1	oxidation					12:20	energy oxidation	5:20	energy oxidation	5:20	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	7	23	theme	months	937:942	arg1	storage					944:950	9 months storage	935:950	9 months storage	935:950	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	2	24	theme	enzyme	300:305	arg1	hydrolyses					307:316	enzyme hydrolyses	300:316	enzyme hydrolyses	300:316	Acid and enzyme hydrolyses are extensively employed to prepare CNC.
30405163	3	25	theme	hazards	430:436	arg1	limitations					405:415	inherent limitations	396:415	inherent limitations of corrosion hazards, time-consuming process, and/or low yield	396:478	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	0	26	theme	High	0:3	arg1	solubilization					37:50	High energy oxidation and organosolv solubilization	0:50	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.	0:133	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	7	27	theme	CNC	1071:1073	arg1	dispersion					1083:1092	the CNC aqueous dispersion	1067:1092	the CNC aqueous dispersion without stirring	1067:1109	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	6	28	theme	stability	701:709	arg1	properties					711:720	The morphology, spectroscopic, and stability properties	666:720	The morphology, spectroscopic, and stability properties of the as-prepared CNC	666:743	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	4	29	theme	rapid	564:568	arg1	degradation					570:580	rapid degradation	564:580	rapid degradation with simultaneous crystallization of cellulose to achieve approx	564:645	Herein, irradiation oxidation and organosolv solubilization are conducted to cause rapid degradation with simultaneous crystallization of cellulose to achieve approx.
30405163	0	30	theme	Eucalyptus	114:123	arg1	hardwood					125:132	Eucalyptus hardwood	114:132	Eucalyptus hardwood	114:132	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	3	31	theme	low	470:472	arg1	yield					474:478	low yield	470:478	low yield	470:478	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	0	32	theme	oxidation	12:20	arg1	solubilization					37:50	High energy oxidation and organosolv solubilization	0:50	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.	0:133	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	1	33	theme	unparalleled	266:277	arg1	properties					279:288	its unparalleled properties	262:288	its unparalleled properties	262:288	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	7	34	dep	solution	1055:1062	arg1	dispersion					1083:1092	the CNC aqueous dispersion	1067:1092	the CNC aqueous dispersion without stirring	1067:1109	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	3	35	theme	yield	474:478	arg1	limitations					405:415	inherent limitations	396:415	inherent limitations of corrosion hazards, time-consuming process, and/or low yield	396:478	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	8	36	theme	lignocellulosic	1193:1207	arg1	biomass					1209:1215	lignocellulosic biomass	1193:1215	lignocellulosic biomass	1193:1215	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	6	37	theme	spectroscopic	682:694	arg1	properties					711:720	The morphology, spectroscopic, and stability properties	666:720	The morphology, spectroscopic, and stability properties of the as-prepared CNC	666:743	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	1	38	theme	responsive	189:198	arg1	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	38	theme	responsive	189:198	arg1	materials					200:208	responsive materials	189:208	responsive materials	189:208	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	3	39	theme	corrosion	420:428	arg1	hazards					430:436	corrosion hazards	420:436	corrosion hazards	420:436	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
30405163	0	40	theme	organosolv	26:35	arg1	solubilization					37:50	High energy oxidation and organosolv solubilization	0:50	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.	0:133	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	8	41	from	approach	1127:1134	arg1	work					1144:1147	this work	1139:1147	this work	1139:1147	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	6	42	theme	zetal	792:796	arg1	potential					798:806	zetal potential	792:806	zetal potential	792:806	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	8	43	theme	acid	1285:1288	arg1	hydrolysis					1290:1299	acid hydrolysis	1285:1299	acid hydrolysis	1285:1299	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	0	44	theme	high	56:59	arg1	isolation					67:75	high yield isolation	56:75	high yield isolation of cellulose nanocrystals (CNC)	56:107	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	6	45	theme	TGA	839:841	arg1	techniques					843:852	TGA techniques	839:852	TGA techniques	839:852	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	7	46	theme	cations	1047:1053	arg1	addition					1025:1032	addition	1025:1032	addition of anions or cations solution to the CNC aqueous dispersion without stirring	1025:1109	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	7	47	theme	anions	1037:1042	arg1	addition					1025:1032	addition	1025:1032	addition of anions or cations solution to the CNC aqueous dispersion without stirring	1025:1109	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	1	48	used	used	181:184	arg2	CNC					159:161	CNC	159:161	CNC	159:161	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	48	used	used	181:184	arg2	materials					200:208	responsive materials	189:208	responsive materials	189:208	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	48	used	used	181:184	arg2	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	48	used	used	181:184	arg2	nano-composites					239:253	tough nano-composites	233:253	tough nano-composites	233:253	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	48	used	used	181:184	arg2	templates					218:226	chiral templates	211:226	chiral templates	211:226	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	6	49	theme	UV-vis	771:776	arg1	spectroscopy					778:789	UV-vis spectroscopy	771:789	UV-vis spectroscopy	771:789	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	6	50	theme	morphology	670:679	arg1	properties					711:720	The morphology, spectroscopic, and stability properties	666:720	The morphology, spectroscopic, and stability properties of the as-prepared CNC	666:743	The morphology, spectroscopic, and stability properties of the as-prepared CNC are characterized through UV-vis spectroscopy, zetal potential, XRD, TEM, DLS, GPC, FT-IR and TGA techniques.
30405163	7	51	theme	liquid	975:980	arg1	phase					990:994	CNC liquid crystal phase	971:994	CNC liquid crystal phase	971:994	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	7	52	theme	unique	893:898	arg1	property					900:907	unique property	893:907	unique property with high stability	893:927	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	0	53	from	hardwood	125:132	arg1	solubilization					37:50	High energy oxidation and organosolv solubilization	0:50	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.	0:133	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	7	54	theme	CNC	869:871	arg1	suspension					873:882	The resultant CNC suspension	855:882	The resultant CNC suspension	855:882	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	7	55	theme	crystal	982:988	arg1	phase					990:994	CNC liquid crystal phase	971:994	CNC liquid crystal phase	971:994	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	1	56	theme	Cellulose	135:143	arg1	templates					218:226	chiral templates	211:226	chiral templates	211:226	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	56	theme	Cellulose	135:143	arg1	nanocrystals					145:156	Cellulose nanocrystals	135:156	Cellulose nanocrystals (CNC)	135:162	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	56	theme	Cellulose	135:143	arg1	materials					200:208	responsive materials	189:208	responsive materials	189:208	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	56	theme	Cellulose	135:143	arg1	nano-composites					239:253	tough nano-composites	233:253	tough nano-composites	233:253	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	1	56	theme	Cellulose	135:143	arg1	CNC					159:161	CNC	159:161	CNC	159:161	Cellulose nanocrystals (CNC) have been widely used as responsive materials, chiral templates, and tough nano-composites due to its unparalleled properties.
30405163	7	57	theme	high	914:917	arg1	stability					919:927	high stability	914:927	high stability	914:927	The resultant CNC suspension presents unique property with high stability after 9 months storage at 4 °C. Moreover, CNC liquid crystal phase is successfully generated by addition of anions or cations solution to the CNC aqueous dispersion without stirring.
30405163	8	58	dep	avenue	1158:1163	arg1	obtain					1168:1173	obtain	1168:1173	to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis	1165:1299	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	8	58	dep	avenue	1158:1163	arg1	washing					1305:1311	washing	1305:1311	washing procedure	1305:1321	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	0	59	theme	yield	61:65	arg1	isolation					67:75	high yield isolation	56:75	high yield isolation of cellulose nanocrystals (CNC)	56:107	High energy oxidation and organosolv solubilization for high yield isolation of cellulose nanocrystals (CNC) from Eucalyptus hardwood.
30405163	8	60	theme	irradiation	1225:1235	arg1	oxidation					1237:1245	irradiation oxidation	1225:1245	irradiation oxidation	1225:1245	The innovative approach in this work opens an avenue to obtain CNC directly from lignocellulosic biomass through irradiation oxidation and organosolv solubilization without acid hydrolysis and washing procedure.
30405163	3	61	theme	traditional	365:375	arg1	approaches					377:386	These traditional approaches	359:386	These traditional approaches	359:386	These traditional approaches exhibit inherent limitations of corrosion hazards, time-consuming process, and/or low yield.
29773402	5	0	theme	AM/propidium	706:717	arg1	staining					726:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	7	1	dep	%	1035:1035	arg1	35					1033:1034	35	1033:1034	35	1033:1034	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	0	2	theme	alveolar	136:143	arg1	repair					150:155	alveolar bone repair	136:155	alveolar bone repair	136:155	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	2	3	theme	HPMC	282:285	arg1	scaffolds					314:322	HPMC crosslinked chitosan based scaffolds	282:322	HPMC crosslinked chitosan based scaffolds for alveolar bone repair	282:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	5	4	theme	iodide	719:724	arg1	staining					726:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	6	5	theme	Differentiation	877:891	arg1	potential					893:901	Differentiation potential	877:901	Differentiation potential of pre-osteoblast cells	877:925	Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay.
29773402	5	6	theme	Hoechst	736:742	arg1	staining					752:759	Hoechst nuclear staining	736:759	Hoechst nuclear staining	736:759	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	3	7	link	crosslinked	382:392	arg1	foams					400:404	HPMC crosslinked CH/BG foams	377:404	HPMC crosslinked CH/BG foams	377:404	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	0	8	theme	bioactive	90:98	arg1	BG					107:108	BG	107:108	BG	107:108	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	8	theme	bioactive	90:98	arg1	glass					100:104	bioactive glass	90:104	bioactive glass (BG)	90:109	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	7	9	dep	viability	1013:1021	arg1	%					1035:1035	%	1035:1035	%	1035:1035	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	0	10	theme	bone	145:148	arg1	repair					150:155	alveolar bone repair	136:155	alveolar bone repair	136:155	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	6	11	theme	phosphate	965:973	arg1	assay					975:979	alkaline phosphate assay	956:979	alkaline phosphate assay	956:979	Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay.
29773402	7	12	theme	synergistic	1055:1065	arg1	effects					1067:1073	synergistic effects	1055:1073	synergistic effects of components	1055:1087	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	2	13	theme	alveolar	328:335	arg1	repair					342:347	alveolar bone repair	328:347	alveolar bone repair	328:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	8	14	theme	crosslinked	1129:1139	arg1	candidates					1165:1174	potential candidates	1155:1174	potential candidates for alveolar bone repair	1155:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	8	14	theme	crosslinked	1129:1139	arg1	scaffolds					1141:1149	HPMC crosslinked scaffolds	1124:1149	HPMC crosslinked scaffolds	1124:1149	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	8	15	theme	bone	1189:1192	arg1	repair					1194:1199	alveolar bone repair	1180:1199	alveolar bone repair	1180:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	3	16	theme	HPMC	377:380	arg1	foams					400:404	HPMC crosslinked CH/BG foams	377:404	HPMC crosslinked CH/BG foams	377:404	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	0	17	theme	cellulose	20:28	arg1	CH					59:60	CH	59:60	CH	59:60	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	17	theme	cellulose	20:28	arg1	chitosan					49:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan	0:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH)	0:61	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	2	18	link	crosslinked	287:297	arg1	scaffolds					314:322	HPMC crosslinked chitosan based scaffolds	282:322	HPMC crosslinked chitosan based scaffolds for alveolar bone repair	282:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	4	19	from	day	601:603	arg1	analysis					589:596	Cytotoxicity analysis	576:596	Cytotoxicity analysis at day 2, 4 and 8	576:614	Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability.
29773402	5	20	theme	cell	836:839	arg1	growth					841:846	pre-osteoblast cell growth	821:846	pre-osteoblast cell growth	821:846	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	0	21	theme	Hydroxypropylmethyl	0:18	arg1	HPMC					31:34	HPMC	31:34	HPMC	31:34	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	21	theme	Hydroxypropylmethyl	0:18	arg1	cellulose					20:28	Hydroxypropylmethyl cellulose	0:28	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH)	0:61	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	6	22	theme	alkaline	956:963	arg1	assay					975:979	alkaline phosphate assay	956:979	alkaline phosphate assay	956:979	Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay.
29773402	0	23	link	crosslinked	37:47	arg1	CH					59:60	CH	59:60	CH	59:60	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	23	link	crosslinked	37:47	arg1	chitosan					49:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan	0:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH)	0:61	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	2	24	theme	based	308:312	arg1	scaffolds					314:322	HPMC crosslinked chitosan based scaffolds	282:322	HPMC crosslinked chitosan based scaffolds for alveolar bone repair	282:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	5	25	theme	cell	765:768	arg1	assay					779:783	cell adhesion assay	765:783	cell adhesion assay	765:783	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	1	26	theme	optimal	219:225	arg1	ridge					236:240	an optimal alveolar ridge	216:240	an optimal alveolar ridge	216:240	The success of a dental implant relies on the presence of an optimal alveolar ridge.
29773402	3	27	theme	100 mg	502:507	arg1	BG					509:510	100 mg BG	502:510	100 mg BG	502:510	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	4	28	theme	Cytotoxicity	576:587	arg1	analysis					589:596	Cytotoxicity analysis	576:596	Cytotoxicity analysis at day 2, 4 and 8	576:614	Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability.
29773402	3	29	theme	mechanical	465:474	arg1	responses					476:484	mechanical responses	465:484	mechanical responses (0.451 MPa with 100 mg BG)	465:511	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	1	30	theme	alveolar	227:234	arg1	ridge					236:240	an optimal alveolar ridge	216:240	an optimal alveolar ridge	216:240	The success of a dental implant relies on the presence of an optimal alveolar ridge.
29773402	8	31	theme	potential	1155:1163	arg1	candidates					1165:1174	potential candidates	1155:1174	potential candidates for alveolar bone repair	1155:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	8	31	theme	potential	1155:1163	arg1	scaffolds					1141:1149	HPMC crosslinked scaffolds	1124:1149	HPMC crosslinked scaffolds	1124:1149	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	4	32	theme	cellular	678:685	arg1	viability					687:695	cellular viability	678:695	cellular viability	678:695	Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability.
29773402	0	33	theme	crosslinked	37:47	arg1	CH					59:60	CH	59:60	CH	59:60	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	33	theme	crosslinked	37:47	arg1	chitosan					49:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan	0:56	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH)	0:61	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	1	34	theme	ridge	236:240	arg1	presence					204:211	the presence	200:211	the presence of an optimal alveolar ridge	200:240	The success of a dental implant relies on the presence of an optimal alveolar ridge.
29773402	0	35	theme	zinc	115:118	arg1	ZnO					127:129	ZnO	127:129	ZnO	127:129	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	35	theme	zinc	115:118	arg1	oxide					120:124	zinc oxide	115:124	zinc oxide (ZnO)	115:130	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	3	36	theme	SEM	529:531	arg1	analysis					533:540	SEM analysis	529:540	SEM analysis	529:540	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	3	37	theme	crosslinked	382:392	arg1	foams					400:404	HPMC crosslinked CH/BG foams	377:404	HPMC crosslinked CH/BG foams	377:404	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	8	38	theme	alveolar	1180:1187	arg1	repair					1194:1199	alveolar bone repair	1180:1199	alveolar bone repair	1180:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	2	39	theme	bone	337:340	arg1	repair					342:347	alveolar bone repair	328:347	alveolar bone repair	328:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	3	40	with	0.451 MPa	487:495	arg1	BG					509:510	100 mg BG	502:510	100 mg BG	502:510	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	8	41	theme	HPMC	1124:1127	arg1	candidates					1165:1174	potential candidates	1155:1174	potential candidates for alveolar bone repair	1155:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	8	41	theme	HPMC	1124:1127	arg1	scaffolds					1141:1149	HPMC crosslinked scaffolds	1124:1149	HPMC crosslinked scaffolds	1124:1149	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	7	42	theme	S.	1003:1004	arg1	viability					1013:1021	S. aureus viability	1003:1021	S. aureus viability as low as 35%	1003:1035	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	3	43	dep	responses	476:484	arg1	0.451 MPa					487:495	0.451 MPa	487:495	0.451 MPa with 100 mg BG	487:510	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	5	44	theme	nuclear	744:750	arg1	staining					752:759	Hoechst nuclear staining	736:759	Hoechst nuclear staining	736:759	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	6	45	theme	cells	921:925	arg1	potential					893:901	Differentiation potential	877:901	Differentiation potential of pre-osteoblast cells	877:925	Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay.
29773402	3	46	theme	better	416:421	arg1	structure					437:445	better morphological structure	416:445	better morphological structure (132-90.5 μm)	416:459	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	3	46	theme	better	416:421	arg1	132-90.5 μm					448:458	132-90.5 μm	448:458	132-90.5 μm	448:458	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	0	47	contain	containing	79:88	arg2	ZnO					127:129	ZnO	127:129	ZnO	127:129	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	47	contain	containing	79:88	arg1	scaffolds					69:77	scaffolds	69:77	scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair	69:155	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	47	contain	containing	79:88	arg2	oxide					120:124	zinc oxide	115:124	zinc oxide (ZnO)	115:130	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	47	contain	containing	79:88	arg2	BG					107:108	BG	107:108	BG	107:108	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	0	47	contain	containing	79:88	arg2	glass					100:104	bioactive glass	90:104	bioactive glass (BG)	90:109	Hydroxypropylmethyl cellulose (HPMC) crosslinked chitosan (CH) based scaffolds containing bioactive glass (BG) and zinc oxide (ZnO) for alveolar bone repair.
29773402	6	48	theme	pre-osteoblast	906:919	arg1	cells					921:925	pre-osteoblast cells	906:925	pre-osteoblast cells	906:925	Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay.
29773402	7	49	theme	aureus	1006:1011	arg1	viability					1013:1021	S. aureus viability	1003:1021	S. aureus viability as low as 35%	1003:1035	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	2	50	theme	study	259:263	arg1	aim					247:249	The aim	243:249	The aim of this study	243:263	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	7	51	theme	viability	1013:1021	arg1	loss					995:998	loss	995:998	loss of S. aureus viability as low as 35%	995:1035	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
29773402	5	52	theme	adhesion	770:777	arg1	assay					779:783	cell adhesion assay	765:783	cell adhesion assay	765:783	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	3	53	theme	morphological	423:435	arg1	structure					437:445	better morphological structure	416:445	better morphological structure (132-90.5 μm)	416:459	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	3	53	theme	morphological	423:435	arg1	132-90.5 μm					448:458	132-90.5 μm	448:458	132-90.5 μm	448:458	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	1	54	theme	dental	175:180	arg1	implant					182:188	a dental implant	173:188	a dental implant	173:188	The success of a dental implant relies on the presence of an optimal alveolar ridge.
29773402	3	55	theme	CH/BG	394:398	arg1	foams					400:404	HPMC crosslinked CH/BG foams	377:404	HPMC crosslinked CH/BG foams	377:404	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	8	56	link	crosslinked	1129:1139	arg1	candidates					1165:1174	potential candidates	1155:1174	potential candidates for alveolar bone repair	1155:1199	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	8	56	link	crosslinked	1129:1139	arg1	scaffolds					1141:1149	HPMC crosslinked scaffolds	1124:1149	HPMC crosslinked scaffolds	1124:1149	Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.
29773402	2	57	theme	chitosan	299:306	arg1	scaffolds					314:322	HPMC crosslinked chitosan based scaffolds	282:322	HPMC crosslinked chitosan based scaffolds for alveolar bone repair	282:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	5	58	theme	pre-osteoblast	821:834	arg1	growth					841:846	pre-osteoblast cell growth	821:846	pre-osteoblast cell growth	821:846	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	1	59	theme	implant	182:188	arg1	success					162:168	The success	158:168	The success of a dental implant	158:188	The success of a dental implant relies on the presence of an optimal alveolar ridge.
29773402	5	60	theme	Calcein	698:704	arg1	staining					726:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining	698:733	Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation.
29773402	3	61	theme	fatigue	546:552	arg1	testing					554:560	fatigue testing	546:560	fatigue testing respectively	546:573	Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively.
29773402	2	62	theme	crosslinked	287:297	arg1	scaffolds					314:322	HPMC crosslinked chitosan based scaffolds	282:322	HPMC crosslinked chitosan based scaffolds for alveolar bone repair	282:347	The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair.
29773402	7	63	theme	components	1078:1087	arg1	effects					1067:1073	synergistic effects	1055:1073	synergistic effects of components	1055:1087	Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components.
31839840	3	0	theme	core	531:534	arg1	structure					536:544	the core structure	527:544	the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages	527:622	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	1	from	MTB	557:559	arg1	structure					536:544	the core structure	527:544	the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages	527:622	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	1	from	MTB	557:559	arg1	AM					549:550	AM	549:550	AM	549:550	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	4	2	theme	branched	808:815	arg1	dodecasaccharides					817:833	branched dodecasaccharides	808:833	branched dodecasaccharides	808:833	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	3	3	from	structure	536:544	arg1	MTB					557:559	MTB	557:559	MTB	557:559	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	2	4	theme	defined	314:320	arg1	oligosaccharides					325:340	defined AM oligosaccharides	314:340	defined AM oligosaccharides	314:340	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	1	5	theme	macrophage	276:285	arg1	activation					287:296	macrophage activation	276:296	macrophage activation	276:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	4	6	theme	reaction	707:714	arg1	conditions					716:725	further optimized reaction conditions	689:725	further optimized reaction conditions	689:725	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	4	7	dep	glycosylation	671:683	arg1	each					666:669	each	666:669	each	666:669	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	4	8	theme	step	655:658	arg1	introduction					629:640	The introduction	625:640	The introduction of a capping step after each glycosylation and further optimized reaction conditions	625:725	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	1	9	theme	Arabinomannan	93:105	arg1	polysaccharides					112:126	Arabinomannan (AM) polysaccharides	93:126	Arabinomannan (AM) polysaccharides	93:126	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	1	9	theme	Arabinomannan	93:105	arg1	biomarkers					141:150	clinical biomarkers	132:150	clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation	132:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	2	10	theme	novel	428:432	arg1	agents					463:468	novel therapeutical and diagnostic agents	428:468	novel therapeutical and diagnostic agents	428:468	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	0	11	theme	Automated	0:8	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of arabinomannan	0:41	Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis.
31839840	1	12	theme	due	200:202	arg1	infections					189:198	Mycobacterium tuberculosis (MTB) infections	156:198	Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation	156:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	0	13	theme	glycan	10:15	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of arabinomannan	0:41	Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis.
31839840	3	14	theme	AM	549:550	arg1	structure					536:544	the core structure	527:544	the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages	527:622	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	1	15	from	interference	258:269	arg1	interaction					226:236	the interaction	222:236	the interaction with host cells	222:252	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	3	16	theme	Automated	471:479	arg1	AGA					498:500	AGA	498:500	AGA	498:500	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	16	theme	Automated	471:479	arg1	assembly					488:495	Automated glycan assembly	471:495	Automated glycan assembly (AGA)	471:501	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	1	17	with	interference	258:269	arg1	activation					287:296	macrophage activation	276:296	macrophage activation	276:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	2	18	theme	oligosaccharides	325:340	arg1	Collections					299:309	Collections	299:309	Collections of defined AM oligosaccharides	299:340	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	2	19	theme	agents	463:468	arg1	understanding					366:378	the understanding	362:378	the understanding of these polysaccharides	362:403	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	2	19	theme	agents	463:468	arg1	development					413:423	the development	409:423	the development of novel therapeutical and diagnostic agents	409:468	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	1	20	with	interaction	226:236	arg1	cells					248:252	host cells	243:252	host cells	243:252	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	2	21	theme	AM	322:323	arg1	oligosaccharides					325:340	defined AM oligosaccharides	314:340	defined AM oligosaccharides	314:340	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	2	22	theme	diagnostic	452:461	arg1	agents					463:468	novel therapeutical and diagnostic agents	428:468	novel therapeutical and diagnostic agents	428:468	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	1	23	with	roles	213:217	arg1	activation					287:296	macrophage activation	276:296	macrophage activation	276:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	0	24	theme	arabinomannan	29:41	arg1	assembly					17:24	Automated glycan assembly	0:24	Automated glycan assembly of arabinomannan	0:41	Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis.
31839840	4	25	theme	series	758:763	arg1	synthesis					743:751	the synthesis	739:751	the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides	739:833	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	2	26	theme	therapeutical	434:446	arg1	agents					463:468	novel therapeutical and diagnostic agents	428:468	novel therapeutical and diagnostic agents	428:468	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	0	27	theme	Mycobacterium	65:77	arg1	tuberculosis					79:90	Mycobacterium tuberculosis	65:90	Mycobacterium tuberculosis	65:90	Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis.
31839840	3	28	theme	glycan	481:486	arg1	AGA					498:500	AGA	498:500	AGA	498:500	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	28	theme	glycan	481:486	arg1	assembly					488:495	Automated glycan assembly	471:495	Automated glycan assembly (AGA)	471:501	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	1	29	theme	clinical	132:139	arg1	polysaccharides					112:126	Arabinomannan (AM) polysaccharides	93:126	Arabinomannan (AM) polysaccharides	93:126	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	1	29	theme	clinical	132:139	arg1	biomarkers					141:150	clinical biomarkers	132:150	clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation	132:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	3	30	contain	containing	562:571	arg2	-Man					580:583	-Man	580:583	-Man	580:583	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	30	contain	containing	562:571	arg2	-Man					610:613	-Man	610:613	-Man	610:613	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	30	contain	containing	562:571	arg2	-Ara					593:596	-Ara	593:596	-Ara	593:596	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	3	30	contain	containing	562:571	arg1	AM					549:550	AM	549:550	AM	549:550	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	4	31	theme	oligosaccharides	768:783	arg1	series					758:763	a series	756:763	a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides	756:833	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	1	32	from	roles	213:217	arg1	interaction					226:236	the interaction	222:236	the interaction with host cells	222:252	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	4	33	theme	capping	647:653	arg1	step					655:658	a capping step	645:658	a capping step after each glycosylation and further optimized reaction conditions	645:725	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	4	34	theme	optimized	697:705	arg1	conditions					716:725	further optimized reaction conditions	689:725	further optimized reaction conditions	689:725	The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.
31839840	1	35	theme	Mycobacterium	156:168	arg1	MTB					184:186	MTB	184:186	MTB	184:186	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	1	35	theme	Mycobacterium	156:168	arg1	tuberculosis					170:181	Mycobacterium tuberculosis	156:181	Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation	156:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	1	36	theme	host	243:246	arg1	cells					248:252	host cells	243:252	host cells	243:252	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	3	37	dep	-Man	580:583	arg1	linkages					615:622	linkages	615:622	linkages	615:622	Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing α-(1,6)-Man, α-(1,5)-Ara, and α-(1,2)-Man linkages.
31839840	1	38	theme	tuberculosis	170:181	arg1	infections					189:198	Mycobacterium tuberculosis (MTB) infections	156:198	Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation	156:296	Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation.
31839840	2	39	theme	polysaccharides	389:403	arg1	understanding					366:378	the understanding	362:378	the understanding of these polysaccharides	362:403	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31839840	2	39	theme	polysaccharides	389:403	arg1	development					413:423	the development	409:423	the development of novel therapeutical and diagnostic agents	409:468	Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents.
31498314	8	0	with	analysis	1246:1253	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	1	theme	fatty	1340:1344	arg1	SCFAs					1353:1357	SCFAs	1353:1357	SCFAs	1353:1357	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	1	theme	fatty	1340:1344	arg1	acids					1346:1350	short-chain fatty acids	1328:1350	short-chain fatty acids (SCFAs)	1328:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	0	2	theme	In	76:77	arg1	Systems					103:109	In Vitro Bath Fermentation Systems	76:109	In Vitro Bath Fermentation Systems	76:109	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	3	3	theme	economic	505:512	arg1	constraints					514:524	economic constraints	505:524	economic constraints	505:524	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	9	4	theme	fermentation	1488:1499	arg1	systems					1501:1507	in vitro batch fermentation systems	1473:1507	in vitro batch fermentation systems	1473:1507	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	6	5	dep	endobiotics	910:920	arg1	microbiota					930:939	microbiota	930:939	microbiota	930:939	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	0	6	theme	Fermentation	90:101	arg1	Systems					103:109	In Vitro Bath Fermentation Systems	76:109	In Vitro Bath Fermentation Systems	76:109	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	0	7	theme	Bath	85:88	arg1	Systems					103:109	In Vitro Bath Fermentation Systems	76:109	In Vitro Bath Fermentation Systems	76:109	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	8	8	with	chromatography	1190:1203	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	9	theme	acids	1346:1350	arg1	chromatography					1190:1203	thin-layer chromatography	1179:1203	thin-layer chromatography (TLC)	1179:1209	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	9	theme	acids	1346:1350	arg1	analysis					1246:1253	bacterial community compositional analysis	1212:1253	bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing	1212:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	9	theme	acids	1346:1350	arg1	TLC					1206:1208	TLC	1206:1208	TLC	1206:1208	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	9	theme	acids	1346:1350	arg1	chromatography					1310:1323	gas chromatography	1306:1323	gas chromatography of short-chain fatty acids (SCFAs)	1306:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	10	theme	gut	1116:1118	arg1	microbiota					1120:1129	human gut microbiota	1110:1129	human gut microbiota	1110:1129	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	10	11	theme	general	1573:1579	arg1	interactions					1581:1592	general interactions	1573:1592	general interactions between other endobiotics and gut microbiota	1573:1637	Importantly, this protocol can also be modified to investigate general interactions between other endobiotics and gut microbiota.
31498314	1	12	theme	Human	112:116	arg1	microorganisms					129:142	Human intestinal microorganisms	112:142	Human intestinal microorganisms	112:142	Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases.
31498314	1	13	theme	human	210:214	arg1	health					216:221	human health	210:221	human health	210:221	Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases.
31498314	9	14	theme	gut	1452:1454	arg1	microbiota					1456:1465	human gut microbiota	1446:1465	human gut microbiota	1446:1465	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	8	15	theme	compositional	1232:1244	arg1	analysis					1246:1253	bacterial community compositional analysis	1212:1253	bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing	1212:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	0	16	theme	Human	49:53	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota	49:68	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	3	17	dep	in	411:412	arg1	vivo					414:417	vivo	414:417	vivo	414:417	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	6	18	theme	alternative	785:795	arg1	strategy					806:813	An alternative research strategy	782:813	An alternative research strategy	782:813	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	6	18	theme	alternative	785:795	arg1	use					822:824	the use	818:824	the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro	818:948	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	3	19	with	experiments	419:429	arg1	volunteers					442:451	human volunteers	436:451	human volunteers	436:451	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	8	20	theme	community	1222:1230	arg1	analysis					1246:1253	bacterial community compositional analysis	1212:1253	bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing	1212:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	21	theme	thin-layer	1179:1188	arg1	chromatography					1190:1203	thin-layer chromatography	1179:1203	thin-layer chromatography (TLC)	1179:1209	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	21	theme	thin-layer	1179:1188	arg1	TLC					1206:1208	TLC	1206:1208	TLC	1206:1208	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	5	22	theme	animal	623:628	arg1	studies					636:642	animal model studies	623:642	animal model studies	623:642	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	5	23	theme	bioethics	665:673	arg1	considerations					675:688	bioethics considerations	665:688	bioethics considerations	665:688	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	1	24	theme	research	188:195	arg1	target					178:183	an important target	165:183	an important target of research	165:195	Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases.
31498314	8	25	theme	Bif	1098:1100	arg1	EPS					1102:1104	Bif EPS	1098:1104	Bif EPS	1098:1104	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	5	26	theme	model	630:634	arg1	studies					636:642	animal model studies	623:642	animal model studies	623:642	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	0	27	dep	In	76:77	arg1	Vitro					79:83	Vitro	79:83	Vitro	79:83	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	8	28	theme	16S	1260:1262	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	2	29	theme	research	386:393	arg1	topic					395:399	an important research topic	373:399	an important research topic	373:399	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	4	30	theme	animal	540:545	arg1	result					532:537	a result	530:537	a result	530:537	As a result, animal models have been used to evaluate these interactions in vivo.
31498314	4	30	theme	animal	540:545	arg1	models					547:552	animal models	540:552	animal models	540:552	As a result, animal models have been used to evaluate these interactions in vivo.
31498314	6	31	theme	research	797:804	arg1	strategy					806:813	An alternative research strategy	782:813	An alternative research strategy	782:813	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	6	31	theme	research	797:804	arg1	use					822:824	the use	818:824	the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro	818:948	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	8	32	theme	short-chain	1328:1338	arg1	SCFAs					1353:1357	SCFAs	1353:1357	SCFAs	1353:1357	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	32	theme	short-chain	1328:1338	arg1	acids					1346:1350	short-chain fatty acids	1328:1350	short-chain fatty acids (SCFAs)	1328:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	33	theme	rRNA	1264:1267	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	0	34	theme	Interactions	12:23	arg1	Analysis					0:7	Analysis	0:7	Analysis of Interactions between Endobiotics and Human Gut Microbiota	0:68	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	1	35	theme	important	168:176	arg1	target					178:183	an important target	165:183	an important target of research	165:195	Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases.
31498314	3	36	theme	such	471:474	arg1	studies					476:482	such studies	471:482	such studies due to bioethics and economic constraints	471:524	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	7	37	used	used	1030:1033	arg2	xenobiotic					1055:1064	xenobiotic	1055:1064	xenobiotic	1055:1064	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	37	used	used	1030:1033	arg2	EPS					1020:1022	EPS	1020:1022	EPS	1020:1022	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	37	used	used	1030:1033	arg2	exopolysaccharides					1000:1017	bifidobacterial (Bif) exopolysaccharides	978:1017	bifidobacterial (Bif) exopolysaccharides (EPS)	978:1023	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	2	38	theme	gut	346:348	arg1	microbiota					350:359	gut microbiota	346:359	gut microbiota	346:359	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	10	39	theme	other	1602:1606	arg1	endobiotics					1608:1618	other endobiotics	1602:1618	other endobiotics	1602:1618	Importantly, this protocol can also be modified to investigate general interactions between other endobiotics and gut microbiota.
31498314	3	40	theme	human	436:440	arg1	volunteers					442:451	human volunteers	436:451	human volunteers	436:451	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	8	41	theme	high-throughput	1274:1288	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	9	42	theme	batch	1482:1486	arg1	systems					1501:1507	in vitro batch fermentation systems	1473:1507	in vitro batch fermentation systems	1473:1507	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	8	43	theme	gene	1269:1272	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	0	44	theme	Gut	55:57	arg1	Microbiota					59:68	Human Gut Microbiota	49:68	Human Gut Microbiota	49:68	Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems.
31498314	8	45	theme	several	1155:1161	arg1	chromatography					1190:1203	thin-layer chromatography	1179:1203	thin-layer chromatography (TLC)	1179:1209	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	45	theme	several	1155:1161	arg1	analysis					1246:1253	bacterial community compositional analysis	1212:1253	bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing	1212:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	45	theme	several	1155:1161	arg1	methods					1163:1169	several methods	1155:1169	several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs)	1155:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	8	45	theme	several	1155:1161	arg1	chromatography					1310:1323	gas chromatography	1306:1323	gas chromatography of short-chain fatty acids (SCFAs)	1306:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	6	46	theme	experiments	848:858	arg1	strategy					806:813	An alternative research strategy	782:813	An alternative research strategy	782:813	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	6	46	theme	experiments	848:858	arg1	use					822:824	the use	818:824	the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro	818:948	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	8	47	theme	human	1110:1114	arg1	microbiota					1120:1129	human gut microbiota	1110:1129	human gut microbiota	1110:1129	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	2	48	dep	drugs	320:324	arg1	e.g.					314:317	e.g.	314:317	e.g.	314:317	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	3	49	theme	in	411:412	arg1	experiments					419:429	in vivo experiments	411:429	in vivo experiments with human volunteers	411:451	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	2	50	dep	endobiotics	301:311	arg1	drugs					320:324	drugs	320:324	drugs	320:324	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	2	50	dep	endobiotics	301:311	arg1	prebiotics					330:339	prebiotics	330:339	prebiotics	330:339	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	6	51	theme	fermentation	835:846	arg1	experiments					848:858	batch fermentation experiments	829:858	batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro	829:948	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	6	52	theme	interactions	889:900	arg1	evaluation					871:880	evaluation	871:880	evaluation of the interactions between endobiotics and gut microbiota	871:939	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	9	53	theme	in	1473:1474	arg1	systems					1501:1507	in vitro batch fermentation systems	1473:1507	in vitro batch fermentation systems	1473:1507	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	1	54	theme	intestinal	118:127	arg1	microorganisms					129:142	Human intestinal microorganisms	112:142	Human intestinal microorganisms	112:142	Human intestinal microorganisms have recently become an important target of research in promoting human health and preventing diseases.
31498314	4	55	used	used	564:567	arg2	models					547:552	animal models	540:552	animal models	540:552	As a result, animal models have been used to evaluate these interactions in vivo.
31498314	4	55	used	used	564:567	arg2	result					532:537	a result	530:537	a result	530:537	As a result, animal models have been used to evaluate these interactions in vivo.
31498314	6	56	theme	batch	829:833	arg1	experiments					848:858	batch fermentation experiments	829:858	batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro	829:948	An alternative research strategy is the use of batch fermentation experiments that allow evaluation of the interactions between endobiotics and gut microbiota in vitro.
31498314	2	57	theme	interactions	280:291	arg1	investigations					262:275	investigations	262:275	investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota	262:359	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	8	58	theme	gas	1306:1308	arg1	chromatography					1310:1323	gas chromatography	1306:1323	gas chromatography of short-chain fatty acids (SCFAs)	1306:1358	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	7	59	theme	Bif	995:997	arg1	xenobiotic					1055:1064	xenobiotic	1055:1064	xenobiotic	1055:1064	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	59	theme	Bif	995:997	arg1	EPS					1020:1022	EPS	1020:1022	EPS	1020:1022	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	59	theme	Bif	995:997	arg1	exopolysaccharides					1000:1017	bifidobacterial (Bif) exopolysaccharides	978:1017	bifidobacterial (Bif) exopolysaccharides (EPS)	978:1023	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	3	60	theme	due	484:486	arg1	studies					476:482	such studies	471:482	such studies due to bioethics and economic constraints	471:524	However, in vivo experiments with human volunteers are not ideal for such studies due to bioethics and economic constraints.
31498314	8	61	theme	bacterial	1212:1220	arg1	analysis					1246:1253	bacterial community compositional analysis	1212:1253	bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing	1212:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	5	62	theme	microbiota	748:757	arg1	diversities					733:743	diversities	733:743	diversities	733:743	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	5	62	theme	microbiota	748:757	arg1	compositions					716:727	compositions	716:727	compositions	716:727	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	8	63	with	chromatography	1310:1323	arg1	sequencing					1290:1299	16S rRNA gene high-throughput sequencing	1260:1299	16S rRNA gene high-throughput sequencing	1260:1299	Then, the interactions between Bif EPS and human gut microbiota were investigated using several methods such as thin-layer chromatography (TLC), bacterial community compositional analysis with 16S rRNA gene high-throughput sequencing, and gas chromatography of short-chain fatty acids (SCFAs).
31498314	2	64	theme	important	376:384	arg1	topic					395:399	an important research topic	373:399	an important research topic	373:399	Consequently, investigations of interactions between endobiotics (e.g., drugs and prebiotics) and gut microbiota have become an important research topic.
31498314	9	65	theme	human	1446:1450	arg1	microbiota					1456:1465	human gut microbiota	1446:1465	human gut microbiota	1446:1465	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	5	66	dep	differing	706:714	arg1	addition					694:701	addition	694:701	addition	694:701	Nevertheless, animal model studies are still limited by bioethics considerations, in addition to differing compositions and diversities of microbiota in animals vs. humans.
31498314	9	67	dep	in	1473:1474	arg1	vitro					1476:1480	vitro	1476:1480	vitro	1476:1480	Presented here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.
31498314	7	68	theme	bifidobacterial	978:992	arg1	xenobiotic					1055:1064	xenobiotic	1055:1064	xenobiotic	1055:1064	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	68	theme	bifidobacterial	978:992	arg1	EPS					1020:1022	EPS	1020:1022	EPS	1020:1022	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	7	68	theme	bifidobacterial	978:992	arg1	exopolysaccharides					1000:1017	bifidobacterial (Bif) exopolysaccharides	978:1017	bifidobacterial (Bif) exopolysaccharides (EPS)	978:1023	To evaluate this strategy, bifidobacterial (Bif) exopolysaccharides (EPS) were used as a representative xenobiotic.
31498314	10	69	theme	gut	1624:1626	arg1	microbiota					1628:1637	gut microbiota	1624:1637	gut microbiota	1624:1637	Importantly, this protocol can also be modified to investigate general interactions between other endobiotics and gut microbiota.
31476393	0	0	theme	glycerol	85:92	arg1	ester					94:98	guar gum-PEG/rosin glycerol ester	66:98	guar gum-PEG/rosin glycerol ester	66:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	4	1	contain	containing	899:908	arg2	GG-PEG/RE					915:923	GG-PEG/RE	915:923	GG-PEG/RE	915:923	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	1	contain	containing	899:908	arg2	1:2					910:912	1:2	910:912	1:2 (GG-PEG/RE)	910:924	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	1	contain	containing	899:908	arg1	blend					950:954	an optimum miscible blend	930:954	an optimum miscible blend	930:954	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	1	contain	containing	899:908	arg1	blend					893:897	the blend	889:897	the blend containing 1:2 (GG-PEG/RE)	889:924	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	1	2	theme	polyethylene	139:150	arg1	PEG					160:162	PEG	160:162	PEG	160:162	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	2	theme	polyethylene	139:150	arg1	glycol					152:157	polyethylene glycol	139:157	polyethylene glycol (PEG)	139:163	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	5	3	theme	Wall	1130:1133	arg1	parameter					1135:1143	Wall parameter	1130:1143	Wall parameter	1130:1143	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	5	4	dep	Krigbaum	1117:1124	arg1	the					1113:1115	the	1113:1115	the	1113:1115	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	0	5	theme	gum-PEG/rosin	71:83	arg1	ester					94:98	guar gum-PEG/rosin glycerol ester	66:98	guar gum-PEG/rosin glycerol ester	66:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	1	6	theme	glycol	152:157	arg1	blends					173:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends	115:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle	115:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	7	theme	blends	462:467	arg1	miscibility					443:453	the miscibility	439:453	the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao	439:689	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	0	8	from	Influence	0:8	arg1	viscosity					53:61	the intrinsic viscosity	39:61	the intrinsic viscosity of guar gum-PEG/rosin glycerol ester	39:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	6	9	dep	viscosity	1233:1241	arg1	results					1243:1249	results	1243:1249	results	1243:1249	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	3	10	theme	intrinsic	742:750	arg1	viscosities					752:762	the intrinsic viscosities	738:762	the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g	738:796	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	0	11	theme	ester	94:98	arg1	viscosity					53:61	the intrinsic viscosity	39:61	the intrinsic viscosity of guar gum-PEG/rosin glycerol ester	39:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	1	12	from	ratios	294:299	arg1	irradiation					257:267	ultrasonic irradiation	246:267	ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1)	246:329	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	7	13	theme	infrared	1465:1472	arg1	spectroscopy					1481:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	14	theme	Scanning	1392:1399	arg1	STEM					1435:1438	STEM	1435:1438	STEM	1435:1438	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	14	theme	Scanning	1392:1399	arg1	microscopy					1423:1432	Scanning transmission electron microscopy	1392:1432	Scanning transmission electron microscopy (STEM)	1392:1439	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	6	15	theme	sub-300 nm	1309:1318	arg1	nanoparticles					1330:1342	sub-300 nm GG-PEG/RE nanoparticles	1309:1342	sub-300 nm GG-PEG/RE nanoparticles	1309:1342	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	2	16	theme	nanosystems	573:583	arg1	time					513:516	sonication time	502:516	sonication time	502:516	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	16	theme	nanosystems	573:583	arg1	viscosities					554:564	The intrinsic viscosities	540:564	The intrinsic viscosities of the nanosystems	540:583	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	16	theme	nanosystems	573:583	arg1	temperature					519:529	temperature	519:529	temperature	519:529	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	17	theme	ultrasound	403:412	arg1	irradiation					414:424	ultrasound irradiation	403:424	ultrasound irradiation	403:424	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	7	18	theme	transformed	1453:1463	arg1	spectroscopy					1481:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	19	theme	polymeric	1532:1540	arg1	nanoparticles					1542:1554	the polymeric nanoparticles	1528:1554	the polymeric nanoparticles	1528:1554	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	4	20	theme	optimum	933:939	arg1	blend					950:954	an optimum miscible blend	930:954	an optimum miscible blend	930:954	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	20	theme	optimum	933:939	arg1	blend					893:897	the blend	889:897	the blend containing 1:2 (GG-PEG/RE)	889:924	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	21	theme	miscible	941:948	arg1	blend					950:954	an optimum miscible blend	930:954	an optimum miscible blend	930:954	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	4	21	theme	miscible	941:948	arg1	blend					893:897	the blend	889:897	the blend containing 1:2 (GG-PEG/RE)	889:924	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	1	22	dep	ratios	294:299	arg1	2:1					317:319	2:1	317:319	2:1	317:319	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	22	dep	ratios	294:299	arg1	4:1					326:328	4:1	326:328	4:1	326:328	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	22	dep	ratios	294:299	arg1	1:4					312:314	1:4	312:314	1:4	312:314	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	22	dep	ratios	294:299	arg1	1:2					307:309	1:2	307:309	1:2	307:309	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	22	dep	ratios	294:299	arg1	1:1					302:304	1:1	302:304	1:1	302:304	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	23	from	solution	472:479	arg1	miscibility					443:453	the miscibility	439:453	the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao	439:689	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	4	24	theme	viscosity	859:867	arg1	results					869:875	The viscosity results	855:875	The viscosity results	855:875	The viscosity results reveal that the blend containing 1:2 (GG-PEG/RE) was an optimum miscible blend.
31476393	1	25	theme	ultrasonic	246:255	arg1	irradiation					257:267	ultrasonic irradiation	246:267	ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1)	246:329	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	26	theme	intrinsic	544:552	arg1	viscosities					554:564	The intrinsic viscosities	540:564	The intrinsic viscosities of the nanosystems	540:583	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	27	theme	different	609:617	arg1	Rao					687:689	Rao	687:689	Rao	687:689	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	27	theme	different	609:617	arg1	Kraemer					646:652	Kraemer	646:652	Kraemer	646:652	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	27	theme	different	609:617	arg1	models					619:624	five different models	604:624	five different models	604:624	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	2	27	theme	different	609:617	arg1	Higiro					675:680	Higiro	675:680	Higiro	675:680	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	7	28	theme	transmission	1401:1412	arg1	STEM					1435:1438	STEM	1435:1438	STEM	1435:1438	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	28	theme	transmission	1401:1412	arg1	microscopy					1423:1432	Scanning transmission electron microscopy	1392:1432	Scanning transmission electron microscopy (STEM)	1392:1439	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	1	29	theme	polymer	165:171	arg1	blends					173:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends	115:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle	115:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	0	30	theme	irradiation	24:34	arg1	Influence					0:8	Influence	0:8	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester	0:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	7	31	theme	nanoparticles	1542:1554	arg1	characterization					1508:1523	characterization	1508:1523	characterization of the polymeric nanoparticles	1508:1554	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	0	32	theme	ultrasound	13:22	arg1	irradiation					24:34	ultrasound irradiation	13:34	ultrasound irradiation	13:34	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	5	33	theme	creaming	1078:1085	arg1	CI					1094:1095	CI	1094:1095	CI	1094:1095	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	5	33	theme	creaming	1078:1085	arg1	index					1087:1091	creaming index	1078:1091	creaming index (CI) parameter	1078:1106	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	2	34	from	miscibility	443:453	arg1	solution					472:479	solution	472:479	solution	472:479	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	1	35	theme	Novel	115:119	arg1	GG					131:132	GG	131:132	GG	131:132	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	35	theme	Novel	115:119	arg1	Gum					126:128	Novel Guar Gum	115:128	Novel Guar Gum (GG)	115:133	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	5	36	theme	index	1087:1091	arg1	parameter					1098:1106	creaming index (CI) parameter	1078:1106	creaming index (CI) parameter	1078:1106	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	1	37	theme	different	272:280	arg1	ratios					294:299	different composition ratios	272:299	different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1)	272:329	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	38	with	blends	173:178	arg1	nanoparticle					211:222	rosin glycerol ester (RE) nanoparticle	185:222	rosin glycerol ester (RE) nanoparticle	185:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	5	39	theme	polymeric	990:998	arg1	nanoparticles					1000:1012	the polymeric nanoparticles	986:1012	the polymeric nanoparticles	986:1012	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	5	40	theme	intermolecular	1173:1186	arg1	interactions					1188:1199	the intermolecular interactions	1169:1199	the intermolecular interactions	1169:1199	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	1	41	theme	rosin	185:189	arg1	nanoparticle					211:222	rosin glycerol ester (RE) nanoparticle	185:222	rosin glycerol ester (RE) nanoparticle	185:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	6	42	theme	miscibility-improving	1277:1297	arg1	effect					1299:1304	the miscibility-improving effect	1273:1304	the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles	1273:1342	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	1	43	theme	composition	282:292	arg1	ratios					294:299	different composition ratios	272:299	different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1)	272:329	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	3	44	theme	138,27	767:772	arg1	viscosities					752:762	the intrinsic viscosities	738:762	the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g	738:796	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	5	45	theme	nanoparticles	1000:1012	arg1	behaviour					973:981	The miscibility behaviour	957:981	The miscibility behaviour of the polymeric nanoparticles	957:1012	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	3	46	theme	best	723:726	arg1	Tanglertpaibul-Rao					696:713	The Tanglertpaibul-Rao	692:713	The Tanglertpaibul-Rao	692:713	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	3	46	theme	best	723:726	arg1	model					728:732	the best model	719:732	the best model	719:732	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	1	47	theme	glycerol	191:198	arg1	nanoparticle					211:222	rosin glycerol ester (RE) nanoparticle	185:222	rosin glycerol ester (RE) nanoparticle	185:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	5	48	theme	shape	1060:1064	arg1	υ					1074:1074	υ	1074:1074	υ	1074:1074	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	5	48	theme	shape	1060:1064	arg1	factor					1066:1071	shape factor	1060:1071	shape factor (υ)	1060:1075	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	2	49	dep	miscibility	443:453	arg1	compared					590:597	compared	590:597	were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao	585:689	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	6	50	theme	GG-PEG/RE	1320:1328	arg1	nanoparticles					1330:1342	sub-300 nm GG-PEG/RE nanoparticles	1309:1342	sub-300 nm GG-PEG/RE nanoparticles	1309:1342	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	1	51	theme	Guar	121:124	arg1	GG					131:132	GG	131:132	GG	131:132	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	1	51	theme	Guar	121:124	arg1	Gum					126:128	Novel Guar Gum	115:128	Novel Guar Gum (GG)	115:133	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	0	52	theme	intrinsic	43:51	arg1	viscosity					53:61	the intrinsic viscosity	39:61	the intrinsic viscosity of guar gum-PEG/rosin glycerol ester	39:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	7	53	used	used	1499:1502	arg2	spectroscopy					1481:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	53	used	used	1499:1502	arg2	STEM					1435:1438	STEM	1435:1438	STEM	1435:1438	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	53	used	used	1499:1502	arg2	microscopy					1423:1432	Scanning transmission electron microscopy	1392:1432	Scanning transmission electron microscopy (STEM)	1392:1439	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	5	54	theme	miscibility	961:971	arg1	behaviour					973:981	The miscibility behaviour	957:981	The miscibility behaviour of the polymeric nanoparticles	957:1012	The miscibility behaviour of the polymeric nanoparticles was investigated using the voluminosity (VE), shape factor (υ), creaming index (CI) parameter, and the Krigbaum and Wall parameter (Δb), which account for the intermolecular interactions.
31476393	1	55	theme	Gum	126:128	arg1	blends					173:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends	115:178	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle	115:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	56	from	blends	462:467	arg1	solution					472:479	solution	472:479	solution	472:479	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	6	57	theme	ultrasonic	1373:1382	arg1	effect					1384:1389	the ultrasonic effect	1369:1389	the ultrasonic effect	1369:1389	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	3	58	theme	163,29 dl/g	786:796	arg1	viscosities					752:762	the intrinsic viscosities	738:762	the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g	738:796	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	1	59	theme	ester	200:204	arg1	nanoparticle					211:222	rosin glycerol ester (RE) nanoparticle	185:222	rosin glycerol ester (RE) nanoparticle	185:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	60	theme	sonication	502:511	arg1	time					513:516	sonication time	502:516	sonication time	502:516	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	6	61	theme	nanoparticles	1258:1270	arg1	viscosity					1233:1241	the intrinsic viscosity results	1219:1249	the intrinsic viscosity results of the nanoparticles	1219:1270	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	0	62	theme	guar	66:69	arg1	ester					94:98	guar gum-PEG/rosin glycerol ester	66:98	guar gum-PEG/rosin glycerol ester	66:98	Influence of ultrasound irradiation on the intrinsic viscosity of guar gum-PEG/rosin glycerol ester nanoparticles.
31476393	2	63	theme	intrinsic	336:344	arg1	viscosities					346:356	The intrinsic viscosities	332:356	The intrinsic viscosities of the nanoparticles	332:377	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	7	64	theme	Fourier	1445:1451	arg1	spectroscopy					1481:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	65	theme	FTIR	1475:1478	arg1	spectroscopy					1481:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Fourier transformed infrared (FTIR) spectroscopy	1445:1492	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	3	66	theme	142,94	775:780	arg1	viscosities					752:762	the intrinsic viscosities	738:762	the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g	738:796	The Tanglertpaibul-Rao was the best model and the intrinsic viscosities of 138,27, 142,94 and 163,29 dl/g were reported for GG-PEG/RE (1:1,1:2,1:4), respectively.
31476393	1	67	theme	RE	207:208	arg1	nanoparticle					211:222	rosin glycerol ester (RE) nanoparticle	185:222	rosin glycerol ester (RE) nanoparticle	185:222	Novel Guar Gum (GG) and polyethylene glycol (PEG) polymer blends with rosin glycerol ester (RE) nanoparticle was synthesized under ultrasonic irradiation at different composition ratios (1:1, 1:2, 1:4, 2:1, and 4:1).
31476393	2	68	theme	nanoparticles	365:377	arg1	viscosities					346:356	The intrinsic viscosities	332:356	The intrinsic viscosities of the nanoparticles	332:377	The intrinsic viscosities of the nanoparticles were investigated using ultrasound irradiation to determine the miscibility of the blends in solution as affected by salt, sonication time, temperature, and pH. The intrinsic viscosities of the nanosystems were compared with five different models, including Huggins, Kraemer, Tanglertpaibul-Rao, Higiro, and Rao.
31476393	7	69	theme	electron	1414:1421	arg1	STEM					1435:1438	STEM	1435:1438	STEM	1435:1438	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	7	69	theme	electron	1414:1421	arg1	microscopy					1423:1432	Scanning transmission electron microscopy	1392:1432	Scanning transmission electron microscopy (STEM)	1392:1439	Scanning transmission electron microscopy (STEM) and Fourier transformed infrared (FTIR) spectroscopy were used for characterization of the polymeric nanoparticles.
31476393	6	70	theme	nanoparticles	1330:1342	arg1	effect					1299:1304	the miscibility-improving effect	1273:1304	the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles	1273:1342	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
31476393	6	71	theme	intrinsic	1223:1231	arg1	viscosity					1233:1241	the intrinsic viscosity results	1219:1249	the intrinsic viscosity results of the nanoparticles	1219:1270	When compared to the intrinsic viscosity results of the nanoparticles, the miscibility-improving effect of sub-300 nm GG-PEG/RE nanoparticles is clearly proven due to the ultrasonic effect.
29624378	6	0	theme	erythropoietin	1037:1050	arg1	spectra					966:972	mass spectra	961:972	mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin	961:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	1	1	theme	Hybrid	138:143	arg1	MS					164:165	MS	164:165	MS	164:165	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	1	1	theme	Hybrid	138:143	arg1	spectrometry					150:161	Hybrid mass spectrometry	138:161	Hybrid mass spectrometry (MS)	138:166	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	1	1	theme	Hybrid	138:143	arg1	technique					183:191	an emerging technique	171:191	an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity	171:279	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	6	2	theme	recombinant	1019:1029	arg1	erythropoietin					1037:1050	recombinant human erythropoietin	1019:1050	recombinant human erythropoietin	1019:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	10	3	from	present	1625:1631	arg1	annotation					1640:1649	the annotation	1636:1649	the annotation of a glycoprotein mass spectrum	1636:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	9	4	theme	identical	1473:1481	arg1	glycans					1483:1489	identical glycans	1473:1489	identical glycans	1473:1489	Notably, glycoforms that comprise identical glycans may nevertheless differ in score if those glycans occupy different sites.
29624378	1	5	theme	mass	145:148	arg1	MS					164:165	MS	164:165	MS	164:165	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	1	5	theme	mass	145:148	arg1	spectrometry					150:161	Hybrid mass spectrometry	138:161	Hybrid mass spectrometry (MS)	138:166	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	1	5	theme	mass	145:148	arg1	technique					183:191	an emerging technique	171:191	an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity	171:279	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	4	6	theme	compatible	749:758	arg1	combinations					736:747	glycan combinations	729:747	glycan combinations compatible with these compositions	729:782	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	7	7	theme	single	1216:1221	arg1	mass					1223:1226	a single mass	1214:1226	a single mass frequently	1214:1237	Accordingly, our software reveals that a single mass frequently can be explained by a considerable number of glycoforms.
29624378	10	8	attach	present	1625:1631	arg2	layers					1595:1600	different layers	1585:1600	different layers of complexity that are present in the annotation of a glycoprotein mass spectrum	1585:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	10	8	attach	present	1625:1631	arg1	annotation					1640:1649	the annotation	1636:1649	the annotation of a glycoprotein mass spectrum	1636:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	2	9	theme	hybrid	288:293	arg1	MS					295:296	hybrid MS	288:296	hybrid MS	288:296	Since hybrid MS combines information from different experimental levels, it crucially depends on computational methods.
29624378	6	10	theme	protein	1160:1166	arg1	level					1168:1172	the intact protein level	1149:1172	the intact protein level	1149:1172	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	0	11	theme	Hybrid	78:83	arg1	Data					85:88	Hybrid Data	78:88	Hybrid Data from the Intact Protein and Glycopeptide Level	78:135	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	6	12	theme	emtansine	1004:1012	arg1	spectra					966:972	mass spectra	961:972	mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin	961:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	10	13	theme	glycoprotein	1656:1667	arg1	spectrum					1674:1681	a glycoprotein mass spectrum	1654:1681	a glycoprotein mass spectrum	1654:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	5	14	theme	released	915:922	arg1	analysis					931:938	released glycan analysis	915:938	released glycan analysis	915:938	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	1	15	theme	pronounced	251:260	arg1	microheterogeneity					262:279	pronounced microheterogeneity	251:279	pronounced microheterogeneity	251:279	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	10	16	theme	different	1585:1593	arg1	layers					1595:1600	different layers	1585:1600	different layers of complexity that are present in the annotation of a glycoprotein mass spectrum	1585:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	2	17	from	levels	347:352	arg1	information					307:317	information	307:317	information from different experimental levels	307:352	Since hybrid MS combines information from different experimental levels, it crucially depends on computational methods.
29624378	3	18	theme	post-translational	509:526	arg1	PTMs					543:546	PTMs	543:546	PTMs	543:546	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	3	18	theme	post-translational	509:526	arg1	modifications					528:540	other post-translational modifications	503:540	other post-translational modifications (PTMs)	503:547	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	6	19	theme	data	1097:1100	arg1	integration					1072:1082	integration	1072:1082	integration of bottom-up data	1072:1100	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	10	20	theme	spectrum	1674:1681	arg1	annotation					1640:1649	the annotation	1636:1649	the annotation of a glycoprotein mass spectrum	1636:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	0	21	from	Level	131:135	arg1	Data					85:88	Hybrid Data	78:88	Hybrid Data from the Intact Protein and Glycopeptide Level	78:135	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	10	22	theme	mass	1669:1672	arg1	spectrum					1674:1681	a glycoprotein mass spectrum	1654:1681	a glycoprotein mass spectrum	1654:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	9	23	theme	different	1548:1556	arg1	sites					1558:1562	different sites	1548:1562	different sites	1548:1562	Notably, glycoforms that comprise identical glycans may nevertheless differ in score if those glycans occupy different sites.
29624378	10	24	from	annotation	1640:1649	arg1	present					1625:1631	present	1625:1631	present	1625:1631	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	3	25	theme	deconvoluted	552:563	arg1	spectra					570:576	deconvoluted mass spectra	552:576	deconvoluted mass spectra of intact proteins	552:595	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	7	26	theme	glycoforms	1284:1293	arg1	number					1274:1279	a considerable number	1259:1279	a considerable number of glycoforms	1259:1293	Accordingly, our software reveals that a single mass frequently can be explained by a considerable number of glycoforms.
29624378	3	27	theme	novel	422:426	arg1	tool					437:440	a novel software tool	420:440	a novel software tool	420:440	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	3	27	theme	novel	422:426	arg1	MoFi					443:446	MoFi	443:446	MoFi	443:446	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	6	28	theme	ado-trastuzumab	988:1002	arg1	emtansine					1004:1012	ado-trastuzumab emtansine	988:1012	ado-trastuzumab emtansine	988:1012	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	2	29	dep	different	324:332	arg1	experimental					334:345	experimental	334:345	experimental	334:345	Since hybrid MS combines information from different experimental levels, it crucially depends on computational methods.
29624378	3	30	theme	software	428:435	arg1	tool					437:440	a novel software tool	420:440	a novel software tool	420:440	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	3	30	theme	software	428:435	arg1	MoFi					443:446	MoFi	443:446	MoFi	443:446	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	0	31	theme	Software	8:15	arg1	Tool					17:20	A Software Tool	6:20	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.	0:136	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	4	32	theme	combinations	736:747	arg1	list					721:724	a hierarchical list	706:724	a hierarchical list of glycan combinations compatible with these compositions	706:782	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	1	33	gly	glycoproteins	212:224	arg1	glycoproteins					212:224	glycoproteins	212:224	glycoproteins	212:224	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	6	34	theme	human	1031:1035	arg1	erythropoietin					1037:1050	recombinant human erythropoietin	1019:1050	recombinant human erythropoietin	1019:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	3	35	theme	mass	565:568	arg1	spectra					570:576	deconvoluted mass spectra	552:576	deconvoluted mass spectra of intact proteins	552:595	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	6	36	theme	rituximab	977:985	arg1	spectra					966:972	mass spectra	961:972	mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin	961:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	0	37	theme	Intact	99:104	arg1	Protein					106:112	the Intact Protein	95:112	the Intact Protein	95:112	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	5	38	theme	glycan	924:929	arg1	analysis					931:938	released glycan analysis	915:938	released glycan analysis	915:938	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	0	39	theme	Glycopeptide	118:129	arg1	Level					131:135	Glycopeptide Level	118:135	Glycopeptide Level	118:135	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	4	40	theme	monosaccharide/PTM	643:660	arg1	compositions					662:673	monosaccharide/PTM compositions	643:673	monosaccharide/PTM compositions	643:673	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	3	41	theme	intact	581:586	arg1	proteins					588:595	intact proteins	581:595	intact proteins	581:595	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	2	42	theme	computational	379:391	arg1	methods					393:399	computational methods	379:399	computational methods	379:399	Since hybrid MS combines information from different experimental levels, it crucially depends on computational methods.
29624378	1	43	theme	emerging	174:181	arg1	spectrometry					150:161	Hybrid mass spectrometry	138:161	Hybrid mass spectrometry (MS)	138:166	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	1	43	theme	emerging	174:181	arg1	technique					183:191	an emerging technique	171:191	an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity	171:279	Hybrid mass spectrometry (MS) is an emerging technique for characterizing glycoproteins, which typically display pronounced microheterogeneity.
29624378	10	44	theme	complexity	1605:1614	arg1	layers					1595:1600	different layers	1585:1600	different layers of complexity that are present in the annotation of a glycoprotein mass spectrum	1585:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	3	45	theme	proteins	588:595	arg1	spectra					570:576	deconvoluted mass spectra	552:576	deconvoluted mass spectra of intact proteins	552:595	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	6	46	used	used	1109:1112	arg2	integration					1072:1082	integration	1072:1082	integration of bottom-up data	1072:1100	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	5	47	theme	glycan	868:873	arg1	library					875:881	a glycan library	866:881	a glycan library as derived from glycopeptide or released glycan analysis	866:938	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	0	48	theme	Glycoprotein	37:48	arg1	Spectra					55:61	Glycoprotein Mass Spectra	37:61	Glycoprotein Mass Spectra	37:61	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	6	49	theme	mass	961:964	arg1	spectra					966:972	mass spectra	961:972	mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin	961:1050	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	5	50	attach	derived	886:892	arg1	glycopeptide					899:910	glycopeptide	899:910	glycopeptide	899:910	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	5	50	attach	derived	886:892	arg2	library					875:881	a glycan library	866:881	a glycan library as derived from glycopeptide or released glycan analysis	866:938	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	5	50	attach	derived	886:892	arg1	analysis					931:938	released glycan analysis	915:938	released glycan analysis	915:938	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	8	51	theme	glycan	1420:1425	arg1	abundances					1427:1436	relative glycan abundances	1411:1436	relative glycan abundances	1411:1436	Yet, it simultaneously ranks proteoforms according to their probability, based on a score which is calculated from relative glycan abundances.
29624378	6	52	theme	intact	1153:1158	arg1	level					1168:1172	the intact protein level	1149:1172	the intact protein level	1149:1172	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	4	53	with	compatible	749:758	arg1	compositions					771:782	these compositions	765:782	these compositions	765:782	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	5	54	gly	glycopeptide	899:910	arg2	glycopeptide					899:910	glycopeptide	899:910	glycopeptide	899:910	Importantly, the program only includes those combinations which are supported by a glycan library as derived from glycopeptide or released glycan analysis.
29624378	8	55	theme	relative	1411:1418	arg1	abundances					1427:1436	relative glycan abundances	1411:1436	relative glycan abundances	1411:1436	Yet, it simultaneously ranks proteoforms according to their probability, based on a score which is calculated from relative glycan abundances.
29624378	4	56	theme	hierarchical	708:719	arg1	list					721:724	a hierarchical list	706:724	a hierarchical list of glycan combinations compatible with these compositions	706:782	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	3	57	theme	hybrid	466:471	arg1	data					476:479	hybrid MS data	466:479	hybrid MS data	466:479	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	10	58	located	present	1625:1631	arg2	layers					1595:1600	different layers	1585:1600	different layers of complexity that are present in the annotation of a glycoprotein mass spectrum	1585:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	10	58	located	present	1625:1631	arg1	annotation					1640:1649	the annotation	1636:1649	the annotation of a glycoprotein mass spectrum	1636:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	6	59	theme	bottom-up	1087:1095	arg1	data					1097:1100	bottom-up data	1087:1100	bottom-up data	1087:1100	By applying MoFi to mass spectra of rituximab, ado-trastuzumab emtansine, and recombinant human erythropoietin, we demonstrate how integration of bottom-up data may be used to refine information collected at the intact protein level.
29624378	2	60	theme	different	324:332	arg1	levels					347:352	different experimental levels	324:352	different experimental levels	324:352	Since hybrid MS combines information from different experimental levels, it crucially depends on computational methods.
29624378	4	61	theme	glycan	729:734	arg1	combinations					736:747	glycan combinations	729:747	glycan combinations compatible with these compositions	729:782	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	10	62	gly	glycoprotein	1656:1667	arg1	glycoprotein					1656:1667	a glycoprotein mass spectrum	1654:1681	a glycoprotein mass spectrum	1654:1681	Hence, MoFi exposes different layers of complexity that are present in the annotation of a glycoprotein mass spectrum.
29624378	4	63	theme	search	612:617	arg1	algorithm					619:627	Its two-stage search algorithm	598:627	Its two-stage search algorithm	598:627	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
29624378	7	64	theme	considerable	1261:1272	arg1	number					1274:1279	a considerable number	1259:1279	a considerable number of glycoforms	1259:1293	Accordingly, our software reveals that a single mass frequently can be explained by a considerable number of glycoforms.
29624378	3	65	theme	MS	473:474	arg1	data					476:479	hybrid MS data	466:479	hybrid MS data	466:479	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	0	66	from	Protein	106:112	arg1	Data					85:88	Hybrid Data	78:88	Hybrid Data from the Intact Protein and Glycopeptide Level	78:135	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	0	67	theme	Mass	50:53	arg1	Spectra					55:61	Glycoprotein Mass Spectra	37:61	Glycoprotein Mass Spectra	37:61	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	0	68	dep	MoFi	0:3	arg1	Tool					17:20	A Software Tool	6:20	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.	0:136	MoFi: A Software Tool for Annotating Glycoprotein Mass Spectra by Integrating Hybrid Data from the Intact Protein and Glycopeptide Level.
29624378	3	69	theme	other	503:507	arg1	PTMs					543:546	PTMs	543:546	PTMs	543:546	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	3	69	theme	other	503:507	arg1	modifications					528:540	other post-translational modifications	503:540	other post-translational modifications (PTMs)	503:547	Here, we describe a novel software tool, MoFi, which integrates hybrid MS data to assign glycans and other post-translational modifications (PTMs) in deconvoluted mass spectra of intact proteins.
29624378	4	70	theme	two-stage	602:610	arg1	algorithm					619:627	Its two-stage search algorithm	598:627	Its two-stage search algorithm	598:627	Its two-stage search algorithm first assigns monosaccharide/PTM compositions to each peak and then compiles a hierarchical list of glycan combinations compatible with these compositions.
30834312	6	0	theme	HIV	1257:1259	arg1	antibodies					1274:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	5	1	theme	glycan	1059:1064	arg1	core					1066:1069	the glycan core	1055:1069	the glycan core	1055:1069	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	6	2	theme	neutralizing	1261:1272	arg1	antibodies					1274:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	1	3	theme	HIV	313:315	arg1	protein					326:332	the HIV envelope protein	309:332	the HIV envelope protein	309:332	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	4	theme	envelope	317:324	arg1	protein					326:332	the HIV envelope protein	309:332	the HIV envelope protein	309:332	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	2	5	theme	neutralization	541:554	arg1	epitope					556:562	this conserved neutralization epitope	526:562	this conserved neutralization epitope	526:562	Thus, there have been numerous attempts to develop glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope.
30834312	5	6	theme	immunogen	1131:1139	arg1	trimming					1115:1122	extensive serum mannosidase trimming	1087:1122	extensive serum mannosidase trimming of the immunogen	1087:1139	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	1	7	theme	protein	326:332	arg1	gp120					300:304	gp120	300:304	gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP)	300:373	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	2	8	theme	conserved	531:539	arg1	epitope					556:562	this conserved neutralization epitope	526:562	this conserved neutralization epitope	526:562	Thus, there have been numerous attempts to develop glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope.
30834312	1	9	dep	∼20	116:118	arg1	to					113:114	to	113:114	to	113:114	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	10	theme	neutralizing	176:187	arg1	bnAbs					201:205	bnAbs	201:205	bnAbs	201:205	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	10	theme	neutralizing	176:187	arg1	antibodies					189:198	broadly neutralizing antibodies	168:198	broadly neutralizing antibodies (bnAbs)	168:206	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	0	11	theme	Glycopeptide	13:24	arg1	Conjugates					26:35	Oligomannose Glycopeptide Conjugates	0:35	Oligomannose Glycopeptide Conjugates	0:35	Oligomannose Glycopeptide Conjugates Elicit Antibodies Targeting the Glycan Core Rather than Its Extremities.
30834312	6	12	theme	glycan-dependent	1240:1255	arg1	antibodies					1274:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	glycan-dependent HIV neutralizing antibodies	1240:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	5	13	theme	Antibody	1030:1037	arg1	specificity					1039:1049	Antibody specificity	1030:1049	Antibody specificity for the glycan core	1030:1069	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	4	14	theme	whole	1007:1011	arg1	PGT128					1021:1026	PGT128	1021:1026	PGT128	1021:1026	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	14	theme	whole	1007:1011	arg1	glycan					1013:1018	the whole glycan	1003:1018	the whole glycan (PGT128)	1003:1027	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	0	15	theme	Oligomannose	0:11	arg1	Conjugates					26:35	Oligomannose Glycopeptide Conjugates	0:35	Oligomannose Glycopeptide Conjugates	0:35	Oligomannose Glycopeptide Conjugates Elicit Antibodies Targeting the Glycan Core Rather than Its Extremities.
30834312	3	16	dep	in	635:636	arg1	vitro					638:642	vitro	638:642	vitro	638:642	Herein, we report on the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12.
30834312	1	17	from	region	254:259	arg1	gp120					300:304	gp120	300:304	gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP)	300:373	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	6	18	theme	vaccine	1208:1214	arg1	design					1216:1221	vaccine design	1208:1221	vaccine design aiming to target glycan-dependent HIV neutralizing antibodies	1208:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	3	19	theme	anti-HMP	676:683	arg1	antibody					685:692	anti-HMP antibody 2G12	676:697	anti-HMP antibody 2G12	676:697	Herein, we report on the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12.
30834312	4	20	theme	fine	717:720	arg1	specificity					735:745	the fine carbohydrate specificity	713:745	the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens	713:795	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	1	21	theme	dense	264:268	arg1	glycosylation					283:295	dense high-mannose glycosylation	264:295	dense high-mannose glycosylation	264:295	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	22	theme	high-mannose	270:281	arg1	glycosylation					283:295	dense high-mannose glycosylation	264:295	dense high-mannose glycosylation	264:295	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	6	23	theme	broad	1185:1189	arg1	implications					1191:1202	broad implications	1185:1202	broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies	1185:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	1	24	theme	high-mannose	349:360	arg1	HMP					370:372	HMP	370:372	HMP	370:372	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	24	theme	high-mannose	349:360	arg1	"					367:367	the "high-mannose patch"	344:367	the "high-mannose patch" (HMP)	344:373	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	4	25	theme	antibodies	757:766	arg1	specificity					735:745	the fine carbohydrate specificity	713:745	the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens	713:795	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	0	26	theme	Glycan	69:74	arg1	Core					76:79	the Glycan Core	65:79	the Glycan Core	65:79	Oligomannose Glycopeptide Conjugates Elicit Antibodies Targeting the Glycan Core Rather than Its Extremities.
30834312	1	27	theme	glycosylation	283:295	arg1	region					254:259	a region	252:259	a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP)	252:373	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	4	28	theme	natural	834:840	arg1	bnAbs					848:852	some natural human bnAbs	829:852	some natural human bnAbs	829:852	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	28	theme	natural	834:840	arg1	PGT128					872:877	PGT128	872:877	PGT128	872:877	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	28	theme	natural	834:840	arg1	2G12					863:866	2G12	863:866	2G12	863:866	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	1	29	theme	patch	362:366	arg1	HMP					370:372	HMP	370:372	HMP	370:372	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	29	theme	patch	362:366	arg1	"					367:367	the "high-mannose patch"	344:367	the "high-mannose patch" (HMP)	344:373	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	4	30	theme	core	915:918	arg1	structures					920:929	the core structures	911:929	the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128)	911:1027	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	31	theme	→	975:975	arg1	2G12					991:994	2G12	991:994	2G12	991:994	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	31	theme	→	975:975	arg1	termini					982:988	the Manα1 → 2Man termini	965:988	the Manα1 → 2Man termini (2G12)	965:995	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	3	32	theme	in	635:636	arg1	selection					644:652	in vitro selection	635:652	in vitro selection	635:652	Herein, we report on the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12.
30834312	4	33	theme	Manα1	969:973	arg1	2G12					991:994	2G12	991:994	2G12	991:994	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	33	theme	Manα1	969:973	arg1	termini					982:988	the Manα1 → 2Man termini	965:988	the Manα1 → 2Man termini (2G12)	965:995	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	34	theme	human	842:846	arg1	bnAbs					848:852	some natural human bnAbs	829:852	some natural human bnAbs	829:852	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	34	theme	human	842:846	arg1	PGT128					872:877	PGT128	872:877	PGT128	872:877	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	34	theme	human	842:846	arg1	2G12					863:866	2G12	863:866	2G12	863:866	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	3	35	theme	glycopeptides	608:620	arg1	immunogenicity					590:603	the immunogenicity	586:603	the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12	586:697	Herein, we report on the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12.
30834312	4	36	theme	carbohydrate	722:733	arg1	specificity					735:745	the fine carbohydrate specificity	713:745	the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens	713:795	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	1	37	theme	HIV-infected	124:135	arg1	individuals					137:147	HIV-infected individuals	124:147	HIV-infected individuals	124:147	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	5	38	theme	vaccinated	1148:1157	arg1	animals					1159:1165	the vaccinated animals	1144:1165	the vaccinated animals	1144:1165	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	5	39	theme	extensive	1087:1095	arg1	trimming					1115:1122	extensive serum mannosidase trimming	1087:1122	extensive serum mannosidase trimming of the immunogen	1087:1139	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	2	40	theme	vaccine	442:448	arg1	immunogens					450:459	glycoconjugate vaccine immunogens	427:459	glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope	427:562	Thus, there have been numerous attempts to develop glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope.
30834312	6	41	contain	has	1181:1183	arg1	finding					1173:1179	This finding	1168:1179	This finding	1168:1179	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	6	41	contain	has	1181:1183	arg2	implications					1191:1202	broad implications	1185:1202	broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies	1185:1283	This finding has broad implications for vaccine design aiming to target glycan-dependent HIV neutralizing antibodies.
30834312	4	42	theme	rabbit	750:755	arg1	antibodies					757:766	rabbit antibodies	750:766	rabbit antibodies elicited by these immunogens	750:795	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	1	43	theme	individuals	137:147	arg1	%					119:119	Up to ∼20%	110:119	Up to ∼20% of HIV-infected individuals	110:147	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	43	theme	individuals	137:147	arg1	individuals					137:147	HIV-infected individuals	124:147	HIV-infected individuals	124:147	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	4	44	theme	2Man	977:980	arg1	2G12					991:994	2G12	991:994	2G12	991:994	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	4	44	theme	2Man	977:980	arg1	termini					982:988	the Manα1 → 2Man termini	965:988	the Manα1 → 2Man termini (2G12)	965:995	By analyzing the fine carbohydrate specificity of rabbit antibodies elicited by these immunogens, we found that they differ from some natural human bnAbs, such as 2G12 and PGT128, in that they bind primarily to the core structures within the glycan, rather than to the Manα1 → 2Man termini (2G12) or to the whole glycan (PGT128).
30834312	1	45	theme	antibodies	227:236	arg1	antibodies					227:236	these antibodies	221:236	these antibodies	221:236	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	45	theme	antibodies	227:236	arg1	%					242:242	∼40%	239:242	∼40%	239:242	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	1	45	theme	antibodies	227:236	arg1	many					213:216	many	213:216	many	213:216	Up to ∼20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (∼40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP).
30834312	2	46	theme	glycoconjugate	427:440	arg1	immunogens					450:459	glycoconjugate vaccine immunogens	427:459	glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope	427:562	Thus, there have been numerous attempts to develop glycoconjugate vaccine immunogens that structurally mimic the HMP and might elicit bnAbs targeting this conserved neutralization epitope.
30834312	5	47	theme	mannosidase	1103:1113	arg1	trimming					1115:1122	extensive serum mannosidase trimming	1087:1122	extensive serum mannosidase trimming of the immunogen	1087:1139	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	5	48	theme	serum	1097:1101	arg1	trimming					1115:1122	extensive serum mannosidase trimming	1087:1122	extensive serum mannosidase trimming of the immunogen	1087:1139	Antibody specificity for the glycan core may result from extensive serum mannosidase trimming of the immunogen in the vaccinated animals.
30834312	3	49	gly	glycopeptides	608:620	arg2	glycopeptides					608:620	glycopeptides	608:620	glycopeptides	608:620	Herein, we report on the immunogenicity of glycopeptides, designed by in vitro selection, that bind tightly to anti-HMP antibody 2G12.
31594808	4	0	theme	polysaccharide	835:848	arg1	length					857:862	the average polysaccharide strand length	823:862	the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	823:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	3	1	theme	cell	538:541	arg1	stiffness					543:551	the swarmer cell stiffness	526:551	the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2)	526:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	1	theme	cell	538:541	arg1	rigidity					562:569	bending rigidity	554:569	bending rigidity	554:569	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	5	2	theme	swarmer	1155:1161	arg1	morphology					1173:1182	swarmer cell wall morphology	1155:1182	swarmer cell wall morphology	1155:1182	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	6	3	dep	β-lactams	1442:1450	arg1	β-lactams					1442:1450	the β-lactams cephalexin and penicillin G	1438:1478	the β-lactams cephalexin and penicillin G	1438:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	6	3	dep	β-lactams	1442:1450	arg1	cephalexin					1452:1461	cephalexin	1452:1461	cephalexin	1452:1461	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	6	3	dep	β-lactams	1442:1450	arg1	G					1478:1478	penicillin G	1467:1478	penicillin G	1467:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	4	dep	22	945:946	arg1	to					942:943	to	942:943	to	942:943	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	9	5	theme	wall-targeting	2289:2302	arg1	antibiotics					2304:2314	cell wall-targeting antibiotics	2284:2314	cell wall-targeting antibiotics (e.g., β-lactams)	2284:2332	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	5	theme	wall-targeting	2289:2302	arg1	β-lactams					2323:2331	β-lactams	2323:2331	β-lactams	2323:2331	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	5	6	theme	wall	1168:1171	arg1	morphology					1173:1182	swarmer cell wall morphology	1155:1182	swarmer cell wall morphology	1155:1182	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	9	7	theme	peptidoglycan	2084:2096	arg1	layer					2098:2102	the peptidoglycan layer	2080:2102	the peptidoglycan layer of the cell wall	2080:2119	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	6	8	theme	parahaemolyticus	1205:1220	arg1	cells					1230:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	8	9	theme	nutrients	1972:1980	arg1	sources					1961:1967	access new sources	1950:1967	access new sources of nutrients	1950:1980	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	1	10	theme	Vibrio	207:212	arg1	parahaemolyticus					214:229	Vibrio parahaemolyticus	207:229	Vibrio parahaemolyticus	207:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	10	11	theme	extracellular	2391:2403	arg1	environment					2405:2415	the extracellular environment	2387:2415	the extracellular environment in determining antibiotic doses	2387:2447	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	6	12	theme	penicillin	1467:1476	arg1	β-lactams					1442:1450	the β-lactams cephalexin and penicillin G	1438:1478	the β-lactams cephalexin and penicillin G	1438:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	6	12	theme	penicillin	1467:1476	arg1	G					1478:1478	penicillin G	1467:1478	penicillin G	1467:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	9	13	theme	cell	2111:2114	arg1	wall					2116:2119	the cell wall	2107:2119	the cell wall	2107:2119	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	6	14	theme	cell	1305:1308	arg1	antibiotics					1325:1335	cell wall-modifying antibiotics	1305:1335	cell wall-modifying antibiotics (compared to vegetative cells)	1305:1366	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	5	15	theme	peptidoglycan	1024:1036	arg1	thickness					1038:1046	P. mirabilis peptidoglycan thickness	1011:1046	P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	1011:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	3	16	theme	mirabilis	578:586	arg1	parahaemolyticus					614:629	V. parahaemolyticus	611:629	V. parahaemolyticus (1.0 × 10-22 N m2)	611:648	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	16	theme	mirabilis	578:586	arg1	stiffness					543:551	the swarmer cell stiffness	526:551	the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2)	526:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	16	theme	mirabilis	578:586	arg1	m2					646:647	1.0 × 10-22 N m2	632:647	1.0 × 10-22 N m2	632:647	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	16	theme	mirabilis	578:586	arg1	rigidity					562:569	bending rigidity	554:569	bending rigidity	554:569	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	6	17	with	treatment	1415:1423	arg1	MICs					1430:1433	MICs	1430:1433	MICs of the β-lactams cephalexin and penicillin G	1430:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	18	theme	wall	903:906	arg1	layer					885:889	the peptidoglycan layer	867:889	the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	867:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	6	19	theme	mirabilis	1188:1196	arg1	cells					1230:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	9	20	theme	cell	2208:2211	arg1	stiffness					2213:2221	cell stiffness	2208:2221	cell stiffness	2208:2221	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	10	21	theme	antibiotic	2432:2441	arg1	doses					2443:2447	antibiotic doses	2432:2447	antibiotic doses	2432:2447	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	6	22	theme	susceptible	1290:1300	arg1	-they					1367:1371	susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they	1290:1371	susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they	1290:1371	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	7	23	theme	cell	1543:1546	arg1	susceptibility					1548:1561	cell susceptibility	1543:1561	cell susceptibility to physical and chemical changes in their environment	1543:1615	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	9	24	dep	i.e.	2195:2198	arg1	reduce					2201:2206	reduce	2201:2206	reduce cell stiffness	2201:2221	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	10	25	theme	β-lactam	2464:2471	arg1	antibiotics					2473:2483	β-lactam antibiotics	2464:2483	β-lactam antibiotics	2464:2483	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	4	26	from	layer	885:889	arg1	disaccharides					922:934	28 to 30 disaccharides	913:934	28 to 30 disaccharides	913:934	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	4	27	theme	layer	885:889	arg1	length					857:862	the average polysaccharide strand length	823:862	the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	823:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	8	28	theme	cell	1909:1912	arg1	morphology					1914:1923	their cell morphology	1903:1923	their cell morphology	1903:1923	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	5	29	theme	P.	1011:1012	arg1	thickness					1038:1046	P. mirabilis peptidoglycan thickness	1011:1046	P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	1011:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	1	30	theme	Gram-negative	148:160	arg1	bacteria					176:183	the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	144:229	the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	144:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	7	31	theme	chemotherapies	1660:1673	arg1	parahaemolyticus					1797:1812	Vibrio parahaemolyticus	1790:1812	Vibrio parahaemolyticus	1790:1812	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	7	31	theme	chemotherapies	1660:1673	arg1	development					1641:1651	the development	1637:1651	the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis	1637:1784	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	7	31	theme	chemotherapies	1660:1673	arg1	bacteria					1818:1825	bacteria	1818:1825	bacteria that infect humans	1818:1844	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	5	32	theme	cell	1163:1166	arg1	morphology					1173:1182	swarmer cell wall morphology	1155:1182	swarmer cell wall morphology	1155:1182	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	1	33	theme	bacteria	176:183	arg1	cells					135:139	Swarmer cells	127:139	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	127:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	8	34	dep	adapt	1850:1854	arg1	"					2020:2020	"	2020:2020	"	2020:2020	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	9	35	theme	parahaemolyticus	2163:2178	arg1	cells					2134:2138	swarmer cells	2126:2138	swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness)	2126:2222	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	6	36	theme	vegetative	1350:1359	arg1	cells					1361:1365	vegetative cells	1350:1365	vegetative cells	1350:1365	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	6	37	dep	antibiotics	1325:1335	arg1	compared					1338:1345	compared	1338:1345	compared to vegetative cells	1338:1365	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	38	dep	reduction	738:746	arg1	∼26-fold					780:787	∼26-fold	780:787	∼26-fold	780:787	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	7	39	theme	swarming	1503:1510	arg1	"					1511:1511	"swarming"	1502:1511	"swarming"	1502:1511	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	9	40	theme	flexible	2185:2192	arg1	parahaemolyticus					2163:2178	V. parahaemolyticus	2160:2178	V. parahaemolyticus more flexible	2160:2192	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	0	41	theme	β-Lactam	102:109	arg1	Susceptibility					111:124	β-Lactam Susceptibility	102:124	β-Lactam Susceptibility	102:124	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	8	42	theme	environmental	1859:1871	arg1	changes					1873:1879	environmental changes	1859:1879	environmental changes	1859:1879	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	4	43	from	decrease	811:818	arg1	length					857:862	the average polysaccharide strand length	823:862	the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	823:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	9	44	theme	mirabilis	2146:2154	arg1	cells					2134:2138	swarmer cells	2126:2138	swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness)	2126:2222	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	1	45	dep	Proteus	185:191	arg1	mirabilis					193:201	mirabilis	193:201	mirabilis	193:201	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	0	46	theme	Bacterial	0:8	arg1	Swarming					10:17	Bacterial Swarming	0:17	Bacterial Swarming	0:17	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	5	47	theme	electron	1110:1117	arg1	cryotomography					1119:1132	electron cryotomography	1110:1132	electron cryotomography	1110:1132	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	1	48	theme	polymer	313:319	arg1	surfaces					321:328	polymer surfaces	313:328	polymer surfaces	313:328	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	1	49	theme	Swarmer	127:133	arg1	cells					135:139	Swarmer cells	127:139	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	127:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	7	50	theme	chemical	1579:1586	arg1	changes					1588:1594	physical and chemical changes	1566:1594	physical and chemical changes in their environment	1566:1615	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	2	51	from	increase	405:412	arg1	flexibility					417:427	flexibility	417:427	flexibility	417:427	We demonstrated that the increasing cell length is accompanied by a large increase in flexibility.
31594808	0	52	theme	Cell	73:76	arg1	Stiffness					78:86	Cell Stiffness	73:86	Cell Stiffness	73:86	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	10	53	theme	parahaemolyticus	2556:2571	arg1	cells					2527:2531	swarmer cells	2519:2531	swarmer cells of P. mirabilis and V. parahaemolyticus	2519:2571	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	0	54	theme	Vibrio	49:54	arg1	parahaemolyticus					56:71	Vibrio parahaemolyticus	49:71	Vibrio parahaemolyticus	49:71	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	6	55	dep	likely	1388:1393	arg1	%					1381:1381	∼30%	1378:1381	∼30%	1378:1381	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	5	56	from	changes	1144:1150	arg1	morphology					1173:1182	swarmer cell wall morphology	1155:1182	swarmer cell wall morphology	1155:1182	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	7	57	theme	Vibrio	1790:1795	arg1	parahaemolyticus					1797:1812	Vibrio parahaemolyticus	1790:1812	Vibrio parahaemolyticus	1790:1812	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	7	57	theme	Vibrio	1790:1795	arg1	development					1641:1651	the development	1637:1651	the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis	1637:1784	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	7	57	theme	Vibrio	1790:1795	arg1	bacteria					1818:1825	bacteria	1818:1825	bacteria that infect humans	1818:1844	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	6	58	theme	V.	1202:1203	arg1	cells					1230:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	2	59	theme	increasing	356:365	arg1	length					372:377	the increasing cell length	352:377	the increasing cell length	352:377	We demonstrated that the increasing cell length is accompanied by a large increase in flexibility.
31594808	5	60	dep	1.5 nm	1053:1058	arg1	1.0 nm					1082:1087	1.0 nm	1082:1087	1.0 nm	1082:1087	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	60	dep	1.5 nm	1053:1058	arg1	cells					1098:1102	swarmer cells	1090:1102	swarmer cells	1090:1102	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	60	dep	1.5 nm	1053:1058	arg1	to					1079:1080	to	1079:1080	to	1079:1080	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	3	61	theme	vegetative	662:671	arg1	m2					694:695	1.4 × 10-20 N m2	680:695	1.4 × 10-20 N m2	680:695	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	61	theme	vegetative	662:671	arg1	m2					715:716	2.2 × 10-22 N m2	701:716	2.2 × 10-22 N m2	701:716	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	61	theme	vegetative	662:671	arg1	cells					673:677	vegetative cells	662:677	vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively)	662:731	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	1	62	dep	long	238:241	arg1	100 μm					251:256	100 μm	251:256	100 μm	251:256	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	5	63	theme	Atomic	963:968	arg1	microscopy					976:985	Atomic force microscopy	963:985	Atomic force microscopy	963:985	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	9	64	theme	layer	2098:2102	arg1	thickness					2067:2075	thickness	2067:2075	thickness	2067:2075	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	64	theme	layer	2098:2102	arg1	composition					2051:2061	composition	2051:2061	composition	2051:2061	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	3	65	theme	swarmer	530:536	arg1	stiffness					543:551	the swarmer cell stiffness	526:551	the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2)	526:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	65	theme	swarmer	530:536	arg1	rigidity					562:569	bending rigidity	554:569	bending rigidity	554:569	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	4	66	theme	average	827:833	arg1	length					857:862	the average polysaccharide strand length	823:862	the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	823:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	7	67	theme	hosts	1735:1739	arg1	colonization					1719:1730	the colonization	1715:1730	the colonization of hosts	1715:1739	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	3	68	theme	N	692:692	arg1	m2					694:695	1.4 × 10-20 N m2	680:695	1.4 × 10-20 N m2	680:695	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	68	theme	N	692:692	arg1	cells					673:677	vegetative cells	662:677	vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively)	662:731	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	9	69	theme	cell	2284:2287	arg1	antibiotics					2304:2314	cell wall-targeting antibiotics	2284:2314	cell wall-targeting antibiotics (e.g., β-lactams)	2284:2332	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	69	theme	cell	2284:2287	arg1	β-lactams					2323:2331	β-lactams	2323:2331	β-lactams	2323:2331	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	3	70	dep	cells	673:677	arg1	m2					694:695	1.4 × 10-20 N m2	680:695	1.4 × 10-20 N m2	680:695	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	70	dep	cells	673:677	arg1	cells					673:677	vegetative cells	662:677	vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively)	662:731	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	70	dep	cells	673:677	arg1	m2					715:716	2.2 × 10-22 N m2	701:716	2.2 × 10-22 N m2	701:716	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	4	71	theme	strand	850:855	arg1	length					857:862	the average polysaccharide strand length	823:862	the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	823:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	6	72	theme	swarmer	1222:1228	arg1	cells					1230:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells	1185:1234	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	3	73	theme	bending	554:560	arg1	stiffness					543:551	the swarmer cell stiffness	526:551	the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2)	526:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	73	theme	bending	554:560	arg1	rigidity					562:569	bending rigidity	554:569	bending rigidity	554:569	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	4	74	dep	∼26-fold	780:787	arg1	to					777:778	to	777:778	to	777:778	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	3	75	theme	N	713:713	arg1	cells					673:677	vegetative cells	662:677	vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively)	662:731	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	75	theme	N	713:713	arg1	m2					715:716	2.2 × 10-22 N m2	701:716	2.2 × 10-22 N m2	701:716	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	1	76	from	motility	301:308	arg1	surfaces					321:328	polymer surfaces	313:328	polymer surfaces	313:328	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	3	77	from	differences	511:521	arg1	parahaemolyticus					614:629	V. parahaemolyticus	611:629	V. parahaemolyticus (1.0 × 10-22 N m2)	611:648	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	77	from	differences	511:521	arg1	stiffness					543:551	the swarmer cell stiffness	526:551	the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2)	526:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	77	from	differences	511:521	arg1	m2					646:647	1.0 × 10-22 N m2	632:647	1.0 × 10-22 N m2	632:647	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	77	from	differences	511:521	arg1	rigidity					562:569	bending rigidity	554:569	bending rigidity	554:569	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	7	78	theme	physical	1566:1573	arg1	changes					1588:1594	physical and chemical changes	1566:1594	physical and chemical changes in their environment	1566:1615	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	7	79	from	increase	1531:1538	arg1	susceptibility					1548:1561	cell susceptibility	1543:1561	cell susceptibility to physical and chemical changes in their environment	1543:1615	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	6	80	theme	wall-modifying	1310:1323	arg1	antibiotics					1325:1335	cell wall-modifying antibiotics	1305:1335	cell wall-modifying antibiotics (compared to vegetative cells)	1305:1366	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	0	81	dep	mirabilis	35:43	arg1	Stiffness					78:86	Cell Stiffness	73:86	Cell Stiffness	73:86	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	9	82	theme	wall	2116:2119	arg1	layer					2098:2102	the peptidoglycan layer	2080:2102	the peptidoglycan layer of the cell wall	2080:2119	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	6	83	theme	β-lactams	1442:1450	arg1	MICs					1430:1433	MICs	1430:1433	MICs of the β-lactams cephalexin and penicillin G	1430:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	84	theme	cell	898:901	arg1	wall					903:906	the cell wall	894:906	the cell wall from 28 to 30 disaccharides	894:934	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	1	85	dep	100 μm	251:256	arg1	to					248:249	to	248:249	to	248:249	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	7	86	from	changes	1588:1594	arg1	environment					1605:1615	their environment	1599:1615	their environment	1599:1615	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	9	87	theme	swarmer	2126:2132	arg1	cells					2134:2138	swarmer cells	2126:2138	swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness)	2126:2222	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	3	88	theme	N	601:601	arg1	mirabilis					578:586	P. mirabilis	575:586	P. mirabilis (5.5 × 10-22 N m2)	575:605	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	88	theme	N	601:601	arg1	m2					603:604	5.5 × 10-22 N m2	589:604	5.5 × 10-22 N m2	589:604	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	1	89	from	growth	290:295	arg1	surfaces					321:328	polymer surfaces	313:328	polymer surfaces	313:328	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	6	90	theme	osmotic	1269:1275	arg1	pressure					1277:1284	osmotic pressure	1269:1284	osmotic pressure	1269:1284	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	91	theme	peptidoglycan	871:883	arg1	layer					885:889	the peptidoglycan layer	867:889	the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	867:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	3	92	theme	large	505:509	arg1	differences					511:521	large differences	505:521	large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2)	505:648	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	9	93	from	changes	2036:2042	arg1	thickness					2067:2075	thickness	2067:2075	thickness	2067:2075	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	93	from	changes	2036:2042	arg1	composition					2051:2061	composition	2051:2061	composition	2051:2061	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	5	94	theme	mirabilis	1014:1022	arg1	thickness					1038:1046	P. mirabilis peptidoglycan thickness	1011:1046	P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	1011:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	3	95	theme	V.	611:612	arg1	parahaemolyticus					614:629	V. parahaemolyticus	611:629	V. parahaemolyticus (1.0 × 10-22 N m2)	611:648	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	95	theme	V.	611:612	arg1	m2					646:647	1.0 × 10-22 N m2	632:647	1.0 × 10-22 N m2	632:647	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	1	96	theme	uropathogenic	162:174	arg1	bacteria					176:183	the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	144:229	the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus	144:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	7	97	theme	"	1511:1511	arg1	cost					1494:1497	The adaptive cost	1481:1497	The adaptive cost of "swarming"	1481:1511	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	5	98	from	1.5 nm	1053:1058	arg1	thickness					1038:1046	P. mirabilis peptidoglycan thickness	1011:1046	P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	1011:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	98	from	1.5 nm	1053:1058	arg1	reduction					998:1006	a reduction	996:1006	a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	996:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	99	from	reduction	998:1006	arg1	cells					1072:1076	vegetative cells	1061:1076	vegetative cells	1061:1076	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	99	from	reduction	998:1006	arg1	thickness					1038:1046	P. mirabilis peptidoglycan thickness	1011:1046	P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells)	1011:1103	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	99	from	reduction	998:1006	arg1	1.5 nm					1053:1058	1.5 nm	1053:1058	1.5 nm	1053:1058	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	7	100	theme	new	1656:1658	arg1	chemotherapies					1660:1673	new chemotherapies	1656:1673	new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis	1656:1784	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	10	101	theme	antibiotics	2473:2483	arg1	importance					2363:2372	the importance	2359:2372	the importance of assessing the extracellular environment in determining antibiotic doses	2359:2447	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	10	101	theme	antibiotics	2473:2483	arg1	use					2457:2459	the use	2453:2459	the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus	2453:2571	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	8	102	theme	new	1957:1959	arg1	sources					1961:1967	access new sources	1950:1967	access new sources of nutrients	1950:1980	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	5	103	theme	swarmer	1090:1096	arg1	1.0 nm					1082:1087	1.0 nm	1082:1087	1.0 nm	1082:1087	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	103	theme	swarmer	1090:1096	arg1	cells					1098:1102	swarmer cells	1090:1102	swarmer cells	1090:1102	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	10	104	theme	swarmer	2519:2525	arg1	cells					2527:2531	swarmer cells	2519:2531	swarmer cells of P. mirabilis and V. parahaemolyticus	2519:2571	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	5	105	theme	vegetative	1061:1070	arg1	cells					1072:1076	vegetative cells	1061:1076	vegetative cells	1061:1076	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	5	105	theme	vegetative	1061:1070	arg1	1.5 nm					1053:1058	1.5 nm	1053:1058	1.5 nm	1053:1058	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	4	106	from	reduction	738:746	arg1	rigidity					759:766	bending rigidity	751:766	bending rigidity	751:766	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	8	107	theme	access	1950:1955	arg1	sources					1961:1967	access new sources	1950:1967	access new sources of nutrients	1950:1980	To adapt to environmental changes, these bacteria alter their cell morphology and move collectively to access new sources of nutrients in a process referred to as "swarming."
31594808	4	108	theme	bending	751:757	arg1	rigidity					759:766	bending rigidity	751:766	bending rigidity	751:766	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	3	109	theme	single-cell	468:478	arg1	mechanics					480:488	single-cell mechanics	468:488	single-cell mechanics	468:488	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	9	110	dep	composition	2051:2061	arg1	the					2047:2049	the	2047:2049	the	2047:2049	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	10	111	theme	mirabilis	2539:2547	arg1	cells					2527:2531	swarmer cells	2519:2531	swarmer cells of P. mirabilis and V. parahaemolyticus	2519:2571	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	2	112	theme	large	399:403	arg1	increase					405:412	a large increase	397:412	a large increase in flexibility	397:427	We demonstrated that the increasing cell length is accompanied by a large increase in flexibility.
31594808	0	113	theme	Proteus	27:33	arg1	mirabilis					35:43	Proteus mirabilis	27:43	Proteus mirabilis	27:43	Bacterial Swarming Reduces Proteus mirabilis and Vibrio parahaemolyticus Cell Stiffness and Increases β-Lactam Susceptibility.
31594808	4	114	dep	30	919:920	arg1	to					916:917	to	916:917	to	916:917	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	1	115	dep	bacteria	176:183	arg1	parahaemolyticus					214:229	Vibrio parahaemolyticus	207:229	Vibrio parahaemolyticus	207:229	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	1	115	dep	bacteria	176:183	arg1	Proteus					185:191	Proteus	185:191	Proteus	185:191	Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces.
31594808	7	116	theme	adaptive	1485:1492	arg1	cost					1494:1497	The adaptive cost	1481:1497	The adaptive cost of "swarming"	1481:1511	The adaptive cost of "swarming" was offset by the increase in cell susceptibility to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for the colonization of hosts and for survival.IMPORTANCEProteus mirabilis and Vibrio parahaemolyticus are bacteria that infect humans.
31594808	5	117	theme	force	970:974	arg1	microscopy					976:985	Atomic force microscopy	963:985	Atomic force microscopy	963:985	Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative cells) to 1.0 nm (swarmer cells), and electron cryotomography indicated changes in swarmer cell wall morphology.
31594808	3	118	theme	microfluidic	438:449	arg1	assay					451:455	a microfluidic assay	436:455	a microfluidic assay to measure single-cell mechanics	436:488	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	119	theme	N	644:644	arg1	parahaemolyticus					614:629	V. parahaemolyticus	611:629	V. parahaemolyticus (1.0 × 10-22 N m2)	611:648	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	3	119	theme	N	644:644	arg1	m2					646:647	1.0 × 10-22 N m2	632:647	1.0 × 10-22 N m2	632:647	Using a microfluidic assay to measure single-cell mechanics, we identified large differences in the swarmer cell stiffness (bending rigidity) of P. mirabilis (5.5 × 10-22 N m2) and V. parahaemolyticus (1.0 × 10-22 N m2) compared to vegetative cells (1.4 × 10-20 N m2 and 2.2 × 10-22 N m2, respectively).
31594808	9	120	dep	mirabilis	2146:2154	arg1	i.e.					2195:2198	i.e.	2195:2198	i.e.	2195:2198	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	10	121	theme	V.	2553:2554	arg1	parahaemolyticus					2556:2571	V. parahaemolyticus	2553:2571	V. parahaemolyticus	2553:2571	These results highlight the importance of assessing the extracellular environment in determining antibiotic doses and the use of β-lactam antibiotics for treating infections caused by swarmer cells of P. mirabilis and V. parahaemolyticus.
31594808	2	122	theme	cell	367:370	arg1	length					372:377	the increasing cell length	352:377	the increasing cell length	352:377	We demonstrated that the increasing cell length is accompanied by a large increase in flexibility.
31594808	9	123	theme	V.	2160:2161	arg1	parahaemolyticus					2163:2178	V. parahaemolyticus	2160:2178	V. parahaemolyticus more flexible	2160:2192	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	124	dep	β-lactams	2323:2331	arg1	e.g.					2317:2320	e.g.	2317:2320	e.g.	2317:2320	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	9	125	theme	osmotic	2263:2269	arg1	pressure					2271:2278	osmotic pressure	2263:2278	osmotic pressure	2263:2278	We found that changes in the composition and thickness of the peptidoglycan layer of the cell wall make swarmer cells of P. mirabilis and V. parahaemolyticus more flexible (i.e., reduce cell stiffness) and that they become more sensitive to osmotic pressure and cell wall-targeting antibiotics (e.g., β-lactams).
31594808	6	126	theme	treatment	1415:1423	arg1	h					1410:1410	3 h	1408:1410	3 h of treatment with MICs of the β-lactams cephalexin and penicillin G	1408:1478	P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)-they were ∼30% more likely to die after 3 h of treatment with MICs of the β-lactams cephalexin and penicillin G.
31594808	4	127	from	disaccharides	922:934	arg1	layer					885:889	the peptidoglycan layer	867:889	the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides	867:960	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
31594808	4	127	from	disaccharides	922:934	arg1	wall					903:906	the cell wall	894:906	the cell wall from 28 to 30 disaccharides	894:934	The reduction in bending rigidity (∼2-fold to ∼26-fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28 to 30 disaccharides to 19 to 22 disaccharides.
29442668	0	0	theme	Composites	97:106	arg1	Morphology					33:42	Morphology	33:42	Morphology	33:42	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	0	0	theme	Composites	97:106	arg1	Properties					62:71	Antibacterial Properties	48:71	Antibacterial Properties	48:71	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	8	1	theme	controlled	1515:1524	arg1	morphology					1526:1535	controlled morphology	1515:1535	controlled morphology	1515:1535	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	6	2	theme	synthetic	1009:1017	arg1	routes					1019:1024	The varied synthetic routes	998:1024	The varied synthetic routes	998:1024	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	0	3	theme	Nanoparticles	83:95	arg1	Composites					97:106	Silver Nanoparticles Composites	76:106	Silver Nanoparticles Composites	76:106	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	8	4	theme	antibacterial	1541:1553	arg1	properties					1555:1564	antibacterial properties	1541:1564	antibacterial properties	1541:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	6	5	theme	varied	1002:1007	arg1	routes					1019:1024	The varied synthetic routes	998:1024	The varied synthetic routes	998:1024	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	8	6	with	nanocomposites	1263:1276	arg1	morphology					1294:1303	controlled morphology	1283:1303	controlled morphology	1283:1303	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	5	7	theme	charge	961:966	arg1	terms					910:914	terms	910:914	terms of morphology, chemical composition, surface charge and antibacterial properties	910:995	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	5	8	theme	surface	953:959	arg1	charge					961:966	surface charge	953:966	surface charge	953:966	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	1	9	theme	textile	244:250	arg1	fabrication					252:262	textile fabrication	244:262	textile fabrication	244:262	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	4	10	theme	cellulose	623:631	arg1	CCW					643:645	CCW	643:645	CCW	643:645	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	4	10	theme	cellulose	623:631	arg1	whiskers					633:640	cellulose whiskers	623:640	cellulose whiskers (CCW)	623:646	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	3	11	theme	cellulose	548:556	arg1	influence					535:543	the influence	531:543	the influence	531:543	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	2	12	theme	cellulose	309:317	arg1	nanostructures					319:332	cellulose nanostructures	309:332	cellulose nanostructures	309:332	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	2	13	theme	metal	338:342	arg1	nanoparticles					344:356	metal nanoparticles	338:356	metal nanoparticles	338:356	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	3	14	theme	nanoparticles	473:485	arg1	material					502:509	cellulose-silver nanoparticles (AgNPs) hybrid material	456:509	cellulose-silver nanoparticles (AgNPs) hybrid material	456:509	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	4	15	theme	silver	764:769	arg1	salt					771:774	silver salt	764:774	silver salt using sodium citrate and/or sodium borohydride in the presence of CCW	764:844	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	5	16	theme	synthesized	851:861	arg1	nanocomposites					873:886	The synthesized AgNPs/CCW nanocomposites	847:886	The synthesized AgNPs/CCW nanocomposites	847:886	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	5	17	theme	properties	986:995	arg1	terms					910:914	terms	910:914	terms of morphology, chemical composition, surface charge and antibacterial properties	910:995	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	6	18	theme	size	1099:1102	arg1	terms					1090:1094	terms	1090:1094	terms of size, shape and coalescence	1090:1125	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	4	19	theme	CCW	842:844	arg1	presence					830:837	the presence	826:837	the presence of CCW	826:844	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	5	20	theme	chemical	931:938	arg1	composition					940:950	chemical composition	931:950	chemical composition	931:950	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	2	21	theme	diverse	379:385	arg1	areas					387:391	diverse areas	379:391	diverse areas such as medical and pharmaceutical applications	379:439	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	2	21	theme	diverse	379:385	arg1	applications					428:439	medical and pharmaceutical applications	401:439	medical and pharmaceutical applications	401:439	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	0	22	theme	Cellulose	0:8	arg1	Whiskers					10:17	Cellulose Whiskers	0:17	Cellulose Whiskers	0:17	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	5	23	theme	composition	940:950	arg1	terms					910:914	terms	910:914	terms of morphology, chemical composition, surface charge and antibacterial properties	910:995	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	5	24	theme	AgNPs/CCW	863:871	arg1	nanocomposites					873:886	The synthesized AgNPs/CCW nanocomposites	847:886	The synthesized AgNPs/CCW nanocomposites	847:886	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	6	25	from	characteristics	1071:1085	arg1	terms					1090:1094	terms	1090:1094	terms of size, shape and coalescence	1090:1125	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	8	26	theme	promising	1423:1431	arg1	compound					1433:1440	a promising compound	1421:1440	a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties	1421:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	8	26	theme	promising	1423:1431	arg1	CCW					1414:1416	CCW	1414:1416	CCW	1414:1416	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	2	27	theme	pharmaceutical	413:426	arg1	applications					428:439	medical and pharmaceutical applications	401:439	medical and pharmaceutical applications	401:439	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	5	28	theme	antibacterial	972:984	arg1	properties					986:995	antibacterial properties	972:995	antibacterial properties	972:995	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	6	29	with	AgNPs	1036:1040	arg1	characteristics					1071:1085	different morphological characteristics	1047:1085	different morphological characteristics in terms of size, shape and coalescence	1047:1125	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	2	30	theme	hybrid	280:285	arg1	materials					287:295	hybrid materials	280:295	hybrid materials composed of cellulose nanostructures and metal nanoparticles	280:356	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	1	31	theme	industrial	192:201	arg1	food					228:231	food	228:231	food	228:231	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	1	31	theme	industrial	192:201	arg1	applications					203:214	industrial applications	192:214	industrial applications	192:214	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	1	31	theme	industrial	192:201	arg1	paper					234:238	paper	234:238	paper	234:238	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	1	31	theme	industrial	192:201	arg1	fabrication					252:262	textile fabrication	244:262	textile fabrication	244:262	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	4	32	theme	acid	696:699	arg1	route					712:716	acid hydrolysis route	696:716	acid hydrolysis route	696:716	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	6	33	theme	morphological	1057:1069	arg1	characteristics					1071:1085	different morphological characteristics	1047:1085	different morphological characteristics in terms of size, shape and coalescence	1047:1125	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	4	34	theme	sodium	804:809	arg1	borohydride					811:821	sodium borohydride	804:821	sodium borohydride	804:821	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	8	35	theme	controlled	1283:1292	arg1	morphology					1294:1303	controlled morphology	1283:1303	controlled morphology	1283:1303	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	2	36	theme	medical	401:407	arg1	applications					428:439	medical and pharmaceutical applications	401:439	medical and pharmaceutical applications	401:439	More recently, hybrid materials composed of cellulose nanostructures and metal nanoparticles have been applied in diverse areas such as medical and pharmaceutical applications.
29442668	4	37	theme	cotton	679:684	arg1	fibers					686:691	commercial cotton fibers	668:691	commercial cotton fibers	668:691	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	7	38	theme	distinct	1173:1180	arg1	behaviors					1182:1190	distinct behaviors	1173:1190	distinct behaviors for the tested bacteria	1173:1214	The particularity of each sample resulted in distinct behaviors for the tested bacteria.
29442668	6	39	theme	different	1047:1055	arg1	characteristics					1071:1085	different morphological characteristics	1047:1085	different morphological characteristics in terms of size, shape and coalescence	1047:1125	The varied synthetic routes generated AgNPs with different morphological characteristics in terms of size, shape and coalescence.
29442668	7	40	theme	sample	1154:1159	arg1	particularity					1132:1144	The particularity	1128:1144	The particularity of each sample	1128:1159	The particularity of each sample resulted in distinct behaviors for the tested bacteria.
29442668	8	41	theme	silver	1473:1478	arg1	nanoparticles					1496:1508	silver and other metal nanoparticles	1473:1508	silver and other metal nanoparticles with controlled morphology and antibacterial properties	1473:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	3	42	theme	stabilizer	573:582	arg1	agent					584:588	a stabilizer agent	571:588	a stabilizer agent	571:588	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	1	43	theme	main	124:127	arg1	Cellulose					109:117	Cellulose	109:117	Cellulose	109:117	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	1	43	theme	main	124:127	arg1	component					129:137	the main component	120:137	the main component of plant cell walls	120:157	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	0	44	theme	Antibacterial	48:60	arg1	Properties					62:71	Antibacterial Properties	48:71	Antibacterial Properties	48:71	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	8	45	theme	metal	1490:1494	arg1	nanoparticles					1496:1508	silver and other metal nanoparticles	1473:1508	silver and other metal nanoparticles with controlled morphology and antibacterial properties	1473:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	0	46	dep	Morphology	33:42	arg1	the					29:31	the	29:31	the	29:31	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	3	47	theme	hybrid	495:500	arg1	material					502:509	cellulose-silver nanoparticles (AgNPs) hybrid material	456:509	cellulose-silver nanoparticles (AgNPs) hybrid material	456:509	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	4	48	theme	hydrolysis	701:710	arg1	route					712:716	acid hydrolysis route	696:716	acid hydrolysis route	696:716	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	8	49	theme	other	1484:1488	arg1	nanoparticles					1496:1508	silver and other metal nanoparticles	1473:1508	silver and other metal nanoparticles with controlled morphology and antibacterial properties	1473:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	4	50	theme	commercial	668:677	arg1	fibers					686:691	commercial cotton fibers	668:691	commercial cotton fibers	668:691	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	5	51	theme	morphology	919:928	arg1	terms					910:914	terms	910:914	terms of morphology, chemical composition, surface charge and antibacterial properties	910:995	The synthesized AgNPs/CCW nanocomposites were characterized in terms of morphology, chemical composition, surface charge and antibacterial properties.
29442668	8	52	with	nanoparticles	1496:1508	arg1	morphology					1526:1535	controlled morphology	1515:1535	controlled morphology	1515:1535	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	8	52	with	nanoparticles	1496:1508	arg1	properties					1555:1564	antibacterial properties	1541:1564	antibacterial properties	1541:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	8	53	theme	antibacterial	1318:1330	arg1	effects					1332:1338	antibacterial effects	1318:1338	antibacterial effects	1318:1338	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	8	54	dep	coli	1351:1354	arg1	Gram-negative					1357:1369	Gram-negative	1357:1369	Gram-negative	1357:1369	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	1	55	theme	plant	142:146	arg1	walls					153:157	plant cell walls	142:157	plant cell walls	142:157	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	0	56	theme	Silver	76:81	arg1	Composites					97:106	Silver Nanoparticles Composites	76:106	Silver Nanoparticles Composites	76:106	Cellulose Whiskers Influence the Morphology and Antibacterial Properties of Silver Nanoparticles Composites.
29442668	8	57	theme	nanoparticles	1496:1508	arg1	syntheses					1460:1468	the syntheses	1456:1468	the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties	1456:1564	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	3	58	theme	cellulose-silver	456:471	arg1	AgNPs					488:492	AgNPs	488:492	AgNPs	488:492	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	3	58	theme	cellulose-silver	456:471	arg1	nanoparticles					473:485	cellulose-silver nanoparticles	456:485	cellulose-silver nanoparticles (AgNPs) hybrid material	456:509	In this work, cellulose-silver nanoparticles (AgNPs) hybrid material was synthesized and the influence of cellulose, employed as a stabilizer agent, was investigated.
29442668	1	59	theme	cell	148:151	arg1	walls					153:157	plant cell walls	142:157	plant cell walls	142:157	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	8	60	theme	AgNPs/CCW	1253:1261	arg1	nanocomposites					1263:1276	AgNPs/CCW nanocomposites	1253:1276	AgNPs/CCW nanocomposites with controlled morphology	1253:1303	Syntheses employing CCW resulted in AgNPs/CCW nanocomposites with controlled morphology and improved antibacterial effects against E. coli (Gram-negative) and S. aureus (Gram-positive), indicating CCW as a promising compound to be used in the syntheses of silver and other metal nanoparticles with controlled morphology and antibacterial properties.
29442668	4	61	theme	sodium	782:787	arg1	citrate					789:795	sodium citrate	782:795	sodium citrate	782:795	Specifically, cellulose whiskers (CCW) were extracted from commercial cotton fibers by acid hydrolysis route, while the AgNPs were synthesized by reducing silver salt using sodium citrate and/or sodium borohydride in the presence of CCW.
29442668	1	62	theme	walls	153:157	arg1	Cellulose					109:117	Cellulose	109:117	Cellulose	109:117	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	1	62	theme	walls	153:157	arg1	component					129:137	the main component	120:137	the main component of plant cell walls	120:157	Cellulose, the main component of plant cell walls, is a biopolymer widely used for industrial applications, including, food, paper and textile fabrication.
29442668	7	63	theme	tested	1200:1205	arg1	bacteria					1207:1214	the tested bacteria	1196:1214	the tested bacteria	1196:1214	The particularity of each sample resulted in distinct behaviors for the tested bacteria.
29392427	0	0	theme	hydrated	73:80	arg1	states					82:87	hydrated states	73:87	hydrated states	73:87	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	6	1	contain	had	1085:1087	arg1	hydration					1075:1083	hydration	1075:1083	hydration	1075:1083	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	6	1	contain	had	1085:1087	arg2	effect					1123:1128	a major statistically significant effect	1089:1128	a major statistically significant effect on the mechanical behaviour of all the tested scaffolds	1089:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	6	2	from	effect	1123:1128	arg1	behaviour					1148:1156	the mechanical behaviour	1133:1156	the mechanical behaviour of all the tested scaffolds	1133:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	5	3	theme	cell	928:931	arg1	seeding					933:939	cell seeding	928:939	cell seeding	928:939	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	4	4	theme	poly	581:584	arg1	nanofibers					598:607	poly(DL-lactide) nanofibers	581:607	poly(DL-lactide) nanofibers	581:607	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	8	5	theme	analysing	1332:1340	arg1	scaffolds					1342:1350	analysing scaffolds	1332:1350	analysing scaffolds	1332:1350	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	8	6	from	importance	1318:1327	arg1	state					1388:1392	the dry state	1380:1392	the hydrated rather than the dry state	1355:1392	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	8	6	from	importance	1318:1327	arg1	hydrated					1359:1366	hydrated	1359:1366	hydrated	1359:1366	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	1	7	from	number	140:145	arg1	engineering					168:178	tissue engineering	161:178	tissue engineering	161:178	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	3	8	theme	such	509:512	arg1	materials					526:534	such hydrophilic materials	509:534	such hydrophilic materials	509:534	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	6	9	theme	significant	1111:1121	arg1	effect					1123:1128	a major statistically significant effect	1089:1128	a major statistically significant effect on the mechanical behaviour of all the tested scaffolds	1089:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	3	10	theme	limiting	398:405	arg1	factor					407:412	a significant limiting factor	384:412	a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo	384:542	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	4	11	with	matrix	573:578	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	2	12	theme	due	292:294	arg1	behaviour					269:277	mechanical behaviour	258:277	mechanical behaviour	258:277	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	0	13	theme	states	82:87	arg1	representative					55:68	representative	55:68	representative	55:68	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	6	14	theme	tested	1169:1174	arg1	scaffolds					1176:1184	all the tested scaffolds	1161:1184	all the tested scaffolds	1161:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	7	15	dep	modulus	1199:1205	arg1	The					1187:1189	The	1187:1189	The	1187:1189	The elastic modulus and compressive strength of all the scaffolds decreased by ~95%.
29392427	4	16	theme	microcomputed	762:774	arg1	tomography					776:785	X-ray microcomputed tomography	756:785	X-ray microcomputed tomography	756:785	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	5	17	dep	48 h	963:966	arg1	up					957:958	up	957:958	up	957:958	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	4	18	theme	dry	726:728	arg1	states					743:748	the dry and hydrated states	722:748	the dry and hydrated states	722:748	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	19	theme	infrared	865:872	arg1	spectrometry					874:885	infrared spectrometry	865:885	infrared spectrometry	865:885	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	20	theme	compression	788:798	arg1	tests					800:804	compression tests	788:804	compression tests	788:804	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	20	theme	compression	788:798	arg1	measurement					830:840	hydraulic permeability measurement	807:840	hydraulic permeability measurement	807:840	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	20	theme	compression	788:798	arg1	tests					855:859	degradation tests	843:859	degradation tests	843:859	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	21	theme	different	668:676	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	1	22	theme	studies	150:156	arg1	number					140:145	a number	138:145	a number of studies in tissue engineering	138:178	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	7	23	theme	compressive	1211:1221	arg1	strength					1223:1230	compressive strength	1211:1230	compressive strength	1211:1230	The elastic modulus and compressive strength of all the scaffolds decreased by ~95%.
29392427	5	24	theme	seeding	933:939	arg1	conditions					914:923	the conditions	910:923	the conditions of cell seeding and cultivation up to 48 h and 576 h	910:976	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	2	25	theme	mechanical	258:267	arg1	behaviour					269:277	mechanical behaviour	258:277	mechanical behaviour	258:277	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	3	26	theme	significant	386:396	arg1	factor					407:412	a significant limiting factor	384:412	a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo	384:542	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	6	27	theme	scaffolds	1176:1184	arg1	behaviour					1148:1156	the mechanical behaviour	1133:1156	the mechanical behaviour of all the tested scaffolds	1133:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	7	28	theme	scaffolds	1243:1251	arg1	strength					1223:1230	compressive strength	1211:1230	compressive strength	1211:1230	The elastic modulus and compressive strength of all the scaffolds decreased by ~95%.
29392427	7	28	theme	scaffolds	1243:1251	arg1	modulus					1199:1205	elastic modulus	1191:1205	elastic modulus	1191:1205	The elastic modulus and compressive strength of all the scaffolds decreased by ~95%.
29392427	1	29	used	used	129:132	arg2	scaffolds					109:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	7	30	theme	elastic	1191:1197	arg1	modulus					1199:1205	elastic modulus	1191:1205	elastic modulus	1191:1205	The elastic modulus and compressive strength of all the scaffolds decreased by ~95%.
29392427	1	31	theme	tissue	161:166	arg1	engineering					168:178	tissue engineering	161:178	tissue engineering	161:178	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	0	32	theme	Dry	0:2	arg1	scaffolds					29:37	Dry versus hydrated collagen scaffolds	0:37	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?	0:88	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	4	33	theme	hydrated	734:741	arg1	states					743:748	the dry and hydrated states	722:748	the dry and hydrated states	722:748	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	0	34	theme	collagen	20:27	arg1	scaffolds					29:37	Dry versus hydrated collagen scaffolds	0:37	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?	0:88	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	4	35	theme	permeability	817:828	arg1	tests					800:804	compression tests	788:804	compression tests	788:804	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	35	theme	permeability	817:828	arg1	measurement					830:840	hydraulic permeability measurement	807:840	hydraulic permeability measurement	807:840	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	0	36	dep	scaffolds	29:37	arg1	states					48:53	dry states	44:53	dry states representative of hydrated states	44:87	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	1	37	theme	Collagen	90:97	arg1	scaffolds					109:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	8	38	theme	quantitative	1276:1287	arg1	results					1289:1295	The quantitative results	1272:1295	The quantitative results provided	1272:1304	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	0	39	theme	hydrated	11:18	arg1	scaffolds					29:37	Dry versus hydrated collagen scaffolds	0:37	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?	0:88	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	8	40	theme	such	1467:1470	arg1	materials					1472:1480	such materials	1467:1480	such materials	1467:1480	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	1	41	theme	composite	99:107	arg1	scaffolds					109:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds	90:117	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	4	42	theme	phosphate	618:626	arg1	particles					628:636	calcium phosphate particles	610:636	calcium phosphate particles	610:636	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	43	theme	collagen	564:571	arg1	matrix					573:578	collagen matrix	564:578	collagen matrix	564:578	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	8	44	theme	scaffolds	1342:1350	arg1	importance					1318:1327	the importance	1314:1327	the importance of analysing scaffolds in the hydrated rather than the dry state	1314:1392	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	4	45	theme	sodium	642:647	arg1	hyaluronate					649:659	sodium hyaluronate	642:659	sodium hyaluronate with 8 different material compositions	642:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	6	46	theme	major	1091:1095	arg1	effect					1123:1128	a major statistically significant effect	1089:1128	a major statistically significant effect on the mechanical behaviour of all the tested scaffolds	1089:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	2	47	theme	porous	210:215	arg1	structures					233:242	such highly porous and hydrophilic structures	198:242	such highly porous and hydrophilic structures	198:242	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	3	48	theme	cells	461:465	arg1	differentiation					442:456	differentiation	442:456	differentiation	442:456	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	48	theme	cells	461:465	arg1	applicability					492:504	the overall applicability	480:504	the overall applicability of such hydrophilic materials in vivo	480:542	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	48	theme	cells	461:465	arg1	growth					431:436	growth	431:436	growth	431:436	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	48	theme	cells	461:465	arg1	seeding					422:428	the seeding	418:428	the seeding	418:428	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	0	49	theme	dry	44:46	arg1	states					48:53	dry states	44:53	dry states representative of hydrated states	44:87	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	4	50	theme	degradation	843:853	arg1	tests					800:804	compression tests	788:804	compression tests	788:804	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	50	theme	degradation	843:853	arg1	tests					855:859	degradation tests	843:859	degradation tests	843:859	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	51	with	hyaluronate	649:659	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	52	with	nanofibers	598:607	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	8	53	theme	dry	1384:1386	arg1	state					1388:1392	the dry state	1380:1392	the hydrated rather than the dry state	1355:1392	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	3	54	theme	overall	484:490	arg1	applicability					492:504	the overall applicability	480:504	the overall applicability of such hydrophilic materials in vivo	480:542	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	2	55	theme	such	198:201	arg1	structures					233:242	such highly porous and hydrophilic structures	198:242	such highly porous and hydrophilic structures	198:242	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	5	56	theme	cultivation	945:955	arg1	conditions					914:923	the conditions	910:923	the conditions of cell seeding and cultivation up to 48 h and 576 h	910:976	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	4	57	theme	hydraulic	807:815	arg1	tests					800:804	compression tests	788:804	compression tests	788:804	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	57	theme	hydraulic	807:815	arg1	measurement					830:840	hydraulic permeability measurement	807:840	hydraulic permeability measurement	807:840	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	3	58	from	differences	313:323	arg1	properties					337:346	physical properties	328:346	physical properties following hydration	328:366	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	8	59	theme	real	1440:1443	arg1	environment					1445:1455	the real environment	1436:1455	the real environment for which such materials are designed	1436:1493	The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.
29392427	5	60	theme	morphological	1020:1032	arg1	parameters					1034:1043	the morphological parameters	1016:1043	the morphological parameters	1016:1043	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	6	61	theme	mechanical	1137:1146	arg1	behaviour					1148:1156	the mechanical behaviour	1133:1156	the mechanical behaviour of all the tested scaffolds	1133:1184	Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds.
29392427	4	62	theme	X-ray	756:760	arg1	tomography					776:785	X-ray microcomputed tomography	756:785	X-ray microcomputed tomography	756:785	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	4	63	with	particles	628:636	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	5	64	theme	minor	1000:1004	arg1	effect					1006:1011	a minor effect	998:1011	a minor effect	998:1011	Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability.
29392427	4	65	theme	material	678:685	arg1	compositions					687:698	8 different material compositions	666:698	8 different material compositions	666:698	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	0	66	theme	representative	55:68	arg1	states					48:53	dry states	44:53	dry states representative of hydrated states	44:87	Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?
29392427	2	67	theme	structures	233:242	arg1	hydration					185:193	The hydration	181:193	The hydration of such highly porous and hydrophilic structures	181:242	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	1	68	from	engineering	168:178	arg1	number					140:145	a number	138:145	a number of studies in tissue engineering	138:178	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	3	69	theme	hydrophilic	514:524	arg1	materials					526:534	such hydrophilic materials	509:534	such hydrophilic materials	509:534	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	4	70	theme	calcium	610:616	arg1	particles					628:636	calcium phosphate particles	610:636	calcium phosphate particles	610:636	Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry.
29392427	3	71	theme	physical	328:335	arg1	properties					337:346	physical properties	328:346	physical properties following hydration	328:366	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	2	72	theme	hydrophilic	221:231	arg1	structures					233:242	such highly porous and hydrophilic structures	198:242	such highly porous and hydrophilic structures	198:242	The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling.
29392427	1	73	from	studies	150:156	arg1	engineering					168:178	tissue engineering	161:178	tissue engineering	161:178	Collagen composite scaffolds have been used for a number of studies in tissue engineering.
29392427	3	74	theme	materials	526:534	arg1	differentiation					442:456	differentiation	442:456	differentiation	442:456	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	74	theme	materials	526:534	arg1	applicability					492:504	the overall applicability	480:504	the overall applicability of such hydrophilic materials in vivo	480:542	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	74	theme	materials	526:534	arg1	growth					431:436	growth	431:436	growth	431:436	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
29392427	3	74	theme	materials	526:534	arg1	seeding					422:428	the seeding	418:428	the seeding	418:428	The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo.
30287386	8	0	theme	local	1198:1202	arg1	modulus					1212:1218	local elastic modulus	1198:1218	local elastic modulus	1198:1218	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	0	1	from	organization	29:40	arg1	cuticle					53:59	tergite cuticle	45:59	tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938	45:119	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	6	2	theme	phases	847:852	arg1	distribution					823:834	The distribution	819:834	The distribution of mineral phases at the edges	819:865	The distribution of mineral phases at the edges resembles that of dorsal regions of the tergites.
30287386	10	3	theme	different	1713:1721	arg1	functionality					1732:1744	different skeletal functionality	1713:1744	different skeletal functionality	1713:1744	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	8	4	theme	modulus	1212:1218	arg1	values					1233:1238	the local elastic modulus and hardness values	1194:1238	the local elastic modulus and hardness values	1194:1238	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	8	4	theme	modulus	1212:1218	arg1	lower					1258:1262	lower	1258:1262	lower	1258:1262	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	5	5	theme	cuticle	693:699	arg1	edges					672:676	the anterior and posterior edges	645:676	the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus	645:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	2	6	theme	skeletal	301:308	arg1	elements					310:317	skeletal elements	301:317	skeletal elements	301:317	The edges of skeletal elements are of particular interest.
30287386	8	7	theme	hardness	1224:1231	arg1	values					1233:1238	the local elastic modulus and hardness values	1194:1238	the local elastic modulus and hardness values	1194:1238	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	8	7	theme	hardness	1224:1231	arg1	lower					1258:1262	lower	1258:1262	lower	1258:1262	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	6	8	theme	tergites	907:914	arg1	regions					892:898	dorsal regions	885:898	dorsal regions of the tergites	885:914	The distribution of mineral phases at the edges resembles that of dorsal regions of the tergites.
30287386	6	9	from	edges	861:865	arg1	distribution					823:834	The distribution	819:834	The distribution of mineral phases at the edges	819:865	The distribution of mineral phases at the edges resembles that of dorsal regions of the tergites.
30287386	7	10	theme	calcium	1161:1167	arg1	phosphate					1169:1177	amorphous calcium phosphate	1151:1177	amorphous calcium phosphate	1151:1177	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	8	11	theme	cuticle	1306:1312	arg1	regions					1283:1289	dorsal regions	1276:1289	dorsal regions of the tergite cuticle	1276:1312	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	5	12	theme	edges	672:676	arg1	properties					631:640	local mechanical properties	614:640	local mechanical properties	614:640	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	12	theme	edges	672:676	arg1	structure					556:564	structure	556:564	structure	556:564	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	12	theme	edges	672:676	arg1	composition					576:586	chemical composition	567:586	chemical composition	567:586	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	12	theme	edges	672:676	arg1	organization					597:608	mineral organization	589:608	mineral organization	589:608	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	2	13	theme	particular	326:335	arg1	interest					337:344	particular interest	326:344	particular interest	326:344	The edges of skeletal elements are of particular interest.
30287386	8	14	contain	containing	1363:1372	arg2	layers					1374:1379	layers	1374:1379	layers	1374:1379	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	8	14	contain	containing	1363:1372	arg1	both					1319:1322	both	1319:1322	both	1319:1322	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	8	14	contain	containing	1363:1372	arg1	calcite					1329:1335	calcite	1329:1335	calcite	1329:1335	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	10	15	theme	co-orientation	1597:1610	arg1	strength					1612:1619	co-orientation strength	1597:1619	co-orientation strength	1597:1619	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	5	16	theme	Tylos	734:738	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	11	17	theme	biological	1902:1911	arg1	composites					1913:1922	biological composites	1902:1922	biological composites	1902:1922	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	7	18	theme	calcium	1112:1118	arg1	carbonate					1120:1128	amorphous calcium carbonate	1102:1128	amorphous calcium carbonate	1102:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	5	19	theme	conglobating	708:719	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	7	20	contain	containing	990:999	arg1	calcite					982:988	the calcite	978:988	the calcite containing distal exocuticle	978:1017	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	7	20	contain	containing	990:999	arg2	exocuticle					1008:1017	distal exocuticle	1001:1017	distal exocuticle	1001:1017	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	11	21	theme	organization	1824:1835	arg1	patterns					1804:1811	patterns	1804:1811	patterns of calcite organization	1804:1835	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	7	22	theme	epicuticular	1034:1045	arg1	material					1047:1054	epicuticular material	1034:1054	epicuticular material	1034:1054	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	9	23	theme	single	1511:1516	arg1	calcite					1530:1536	almost single crystalline calcite	1504:1536	(ii) almost single crystalline calcite	1499:1536	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	7	24	theme	unmineralized	944:956	arg1	membrane					969:976	the unmineralized arthrodial membrane	940:976	the unmineralized arthrodial membrane	940:976	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	7	25	theme	cuticular	1074:1082	arg1	layers					1084:1089	the subjacent cuticular layers	1060:1089	the subjacent cuticular layers containing amorphous calcium carbonate	1060:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	1	26	theme	crustacean	126:135	arg1	cuticle					137:143	The crustacean cuticle	122:143	The crustacean cuticle	122:143	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	5	27	theme	dorsal	788:793	arg1	region					795:800	the protective dorsal region	773:800	the protective dorsal region of the tergites	773:816	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	28	theme	beach	721:725	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	29	theme	mechanical	620:629	arg1	properties					631:640	local mechanical properties	614:640	local mechanical properties	614:640	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	7	30	dep	membrane	969:976	arg1	the					920:922	the	920:922	the	920:922	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	7	30	dep	membrane	969:976	arg1	transition					924:933	transition	924:933	transition	924:933	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	11	31	dep	architecture	1868:1879	arg1	the					1851:1853	the	1851:1853	the	1851:1853	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	0	32	theme	Arcangeli	105:113	arg1	cuticle					53:59	tergite cuticle	45:59	tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938	45:119	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	5	33	theme	mineral	589:595	arg1	organization					597:608	mineral organization	589:608	mineral organization	589:608	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	4	34	theme	skeletal	507:514	arg1	elements					516:523	skeletal elements	507:523	skeletal elements	507:523	These allow for relative movements of skeletal elements.
30287386	0	35	theme	Tailored	0:7	arg1	disorder					9:16	Tailored disorder	0:16	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.	0:120	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	7	36	theme	amorphous	1151:1159	arg1	phosphate					1169:1177	amorphous calcium phosphate	1151:1177	amorphous calcium phosphate	1151:1177	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	10	37	theme	micrometres	1670:1680	arg1	tens					1662:1665	very few tens	1653:1665	very few tens of micrometres	1653:1680	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	5	38	theme	tergites	809:816	arg1	region					795:800	the protective dorsal region	773:800	the protective dorsal region of the tergites	773:816	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	0	39	theme	tergite	45:51	arg1	cuticle					53:59	tergite cuticle	45:59	tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938	45:119	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	11	40	theme	hierarchical	1855:1866	arg1	architecture					1868:1879	architecture	1868:1879	architecture	1868:1879	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	1	41	theme	crystalline	234:244	arg1	carbonate					254:262	amorphous and crystalline calcium carbonate	220:262	amorphous and crystalline calcium carbonate	220:262	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	11	42	theme	composites	1913:1922	arg1	functionality					1885:1897	functionality	1885:1897	functionality	1885:1897	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	11	42	theme	composites	1913:1922	arg1	architecture					1868:1879	architecture	1868:1879	architecture	1868:1879	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	9	43	theme	different	1437:1445	arg1	patterns					1455:1462	different texture patterns	1437:1462	different texture patterns	1437:1462	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	5	44	from	cuticle	693:699	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	10	45	theme	skeletal	1723:1730	arg1	functionality					1732:1744	different skeletal functionality	1713:1744	different skeletal functionality	1713:1744	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	5	46	from	europaeus	740:748	arg1	edges					672:676	the anterior and posterior edges	645:676	the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus	645:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	47	theme	tergite	685:691	arg1	cuticle					693:699	the tergite cuticle	681:699	the tergite cuticle in the conglobating beach isopod Tylos europaeus	681:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	6	48	theme	mineral	839:845	arg1	phases					847:852	mineral phases	839:852	mineral phases	839:852	The distribution of mineral phases at the edges resembles that of dorsal regions of the tergites.
30287386	2	49	theme	elements	310:317	arg1	edges					292:296	The edges	288:296	The edges of skeletal elements	288:317	The edges of skeletal elements are of particular interest.
30287386	8	50	theme	elastic	1204:1210	arg1	modulus					1212:1218	local elastic modulus	1198:1218	local elastic modulus	1198:1218	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	10	51	theme	Calcite	1572:1578	arg1	organization					1580:1591	Calcite organization	1572:1591	Calcite organization	1572:1591	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	5	52	theme	anterior	649:656	arg1	edges					672:676	the anterior and posterior edges	645:676	the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus	645:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	9	53	theme	random	1476:1481	arg1	co-orientation					1483:1496	almost random co-orientation	1469:1496	(i) almost random co-orientation	1465:1496	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	5	54	theme	posterior	662:670	arg1	edges					672:676	the anterior and posterior edges	645:676	the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus	645:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	1	55	theme	chitin-protein	183:196	arg1	fibrils					198:204	chitin-protein fibrils	183:204	chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals	183:285	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	8	56	theme	tergite	1298:1304	arg1	cuticle					1306:1312	the tergite cuticle	1294:1312	the tergite cuticle	1294:1312	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	11	57	theme	calcite	1816:1822	arg1	organization					1824:1835	calcite organization	1816:1835	calcite organization	1816:1835	Our results show that besides structure and composition, patterns of calcite organization contribute to the hierarchical architecture and functionality of biological composites.
30287386	5	58	theme	isopod	727:732	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	1	59	dep	carbonate	254:262	arg1	minerals					278:285	minerals	278:285	minerals	278:285	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	7	60	theme	amorphous	1102:1110	arg1	carbonate					1120:1128	amorphous calcium carbonate	1102:1128	amorphous calcium carbonate	1102:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	1	61	theme	amorphous	220:228	arg1	carbonate					254:262	amorphous and crystalline calcium carbonate	220:262	amorphous and crystalline calcium carbonate	220:262	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	4	62	theme	relative	485:492	arg1	movements					494:502	relative movements	485:502	relative movements of skeletal elements	485:523	These allow for relative movements of skeletal elements.
30287386	5	63	theme	chemical	567:574	arg1	composition					576:586	chemical composition	567:586	chemical composition	567:586	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	8	64	theme	dorsal	1276:1281	arg1	regions					1283:1289	dorsal regions	1276:1289	dorsal regions of the tergite cuticle	1276:1312	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	9	65	dep	organization	1558:1569	arg1	iii					1544:1546	iii	1544:1546	iii	1544:1546	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	7	66	theme	subjacent	1064:1072	arg1	layers					1084:1089	the subjacent cuticular layers	1060:1089	the subjacent cuticular layers containing amorphous calcium carbonate	1060:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	9	67	theme	crystalline	1518:1528	arg1	calcite					1530:1536	almost single crystalline calcite	1504:1536	(ii) almost single crystalline calcite	1499:1536	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	5	68	theme	protective	777:786	arg1	region					795:800	the protective dorsal region	773:800	the protective dorsal region of the tergites	773:816	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	1	69	theme	skeletal	151:158	arg1	elements					160:167	skeletal elements	151:167	skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals	151:285	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	0	70	from	disorder	9:16	arg1	organization					29:40	calcite organization	21:40	calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938	21:119	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	7	71	theme	distal	1001:1006	arg1	exocuticle					1008:1017	distal exocuticle	1001:1017	distal exocuticle	1001:1017	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	9	72	dep	calcite	1530:1536	arg1	ii					1500:1501	ii	1500:1501	ii	1500:1501	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	9	73	theme	graded	1551:1556	arg1	organization					1558:1569	a graded organization	1549:1569	(iii) a graded organization	1543:1569	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	3	74	theme	repeated	367:374	arg1	strain					376:381	strain	376:381	strain	376:381	They are subject to repeated strain and stress because they form transitions to the arthrodial membranes connecting them.
30287386	4	75	theme	elements	516:523	arg1	movements					494:502	relative movements	485:502	relative movements of skeletal elements	485:523	These allow for relative movements of skeletal elements.
30287386	7	76	contain	containing	1091:1100	arg1	layers					1084:1089	the subjacent cuticular layers	1060:1089	the subjacent cuticular layers containing amorphous calcium carbonate	1060:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	7	76	contain	containing	1091:1100	arg2	carbonate					1120:1128	amorphous calcium carbonate	1102:1128	amorphous calcium carbonate	1102:1128	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	10	77	theme	few	1658:1660	arg1	tens					1662:1665	very few tens	1653:1665	very few tens of micrometres	1653:1680	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	0	78	theme	calcite	21:27	arg1	organization					29:40	calcite organization	21:40	calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938	21:119	Tailored disorder in calcite organization in tergite cuticle of the supralittoral isopod Tylos europaeus Arcangeli, 1938.
30287386	5	79	from	edges	672:676	arg1	europaeus					740:748	the conglobating beach isopod Tylos europaeus	704:748	the conglobating beach isopod Tylos europaeus	704:748	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	5	80	theme	local	614:618	arg1	properties					631:640	local mechanical properties	614:640	local mechanical properties	614:640	In this study, we investigate structure, chemical composition, mineral organization and local mechanical properties of the anterior and posterior edges of the tergite cuticle in the conglobating beach isopod Tylos europaeus and compare these with the protective dorsal region of the tergites.
30287386	6	81	theme	dorsal	885:890	arg1	regions					892:898	dorsal regions	885:898	dorsal regions of the tergites	885:914	The distribution of mineral phases at the edges resembles that of dorsal regions of the tergites.
30287386	3	82	theme	arthrodial	431:440	arg1	membranes					442:450	the arthrodial membranes	427:450	the arthrodial membranes connecting them	427:466	They are subject to repeated strain and stress because they form transitions to the arthrodial membranes connecting them.
30287386	9	83	dep	co-orientation	1483:1496	arg1	i					1466:1466	i	1466:1466	i	1466:1466	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	9	84	theme	tergite	1405:1411	arg1	cuticle					1413:1419	the tergite cuticle	1401:1419	the tergite cuticle	1401:1419	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
30287386	10	85	with	regions	1700:1706	arg1	functionality					1732:1744	different skeletal functionality	1713:1744	different skeletal functionality	1713:1744	Calcite organization and co-orientation strength is highly variable, not only on very few tens of micrometres, but also between regions with different skeletal functionality.
30287386	7	86	theme	arthrodial	958:967	arg1	membrane					969:976	the unmineralized arthrodial membrane	940:976	the unmineralized arthrodial membrane	940:976	At the transition with the unmineralized arthrodial membrane the calcite containing distal exocuticle is replaced by epicuticular material and the subjacent cuticular layers containing amorphous calcium carbonate become enriched with amorphous calcium phosphate.
30287386	1	87	theme	calcium	246:252	arg1	carbonate					254:262	amorphous and crystalline calcium carbonate	220:262	amorphous and crystalline calcium carbonate	220:262	The crustacean cuticle forms skeletal elements consisting of chitin-protein fibrils reinforced by amorphous and crystalline calcium carbonate and phosphate minerals.
30287386	8	88	dep	calcite	1329:1335	arg1	the					1325:1327	the	1325:1327	the	1325:1327	At the edges, the local elastic modulus and hardness values are significantly lower compared to dorsal regions of the tergite cuticle, for both, the calcite and the amorphous mineral containing layers.
30287386	9	89	theme	texture	1447:1453	arg1	patterns					1455:1462	different texture patterns	1437:1462	different texture patterns	1437:1462	The calcite within the tergite cuticle is assembled in different texture patterns: (i) almost random co-orientation, (ii) almost single crystalline calcite, and (iii) a graded organization.
31845534	6	0	theme	similar	1243:1249	arg1	level					1251:1255	a similar level	1241:1255	a similar level	1241:1255	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	2	1	theme	haemostatic	365:375	arg1	activity					377:384	better haemostatic activity	358:384	better haemostatic activity	358:384	However, there are still urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products.
31845534	1	2	theme	intraoperative	246:259	arg1	conditions					261:270	both prehospital and intraoperative conditions	225:270	both prehospital and intraoperative conditions	225:270	The use of haemostatic agents can provide life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions.
31845534	0	3	theme	topical	73:79	arg1	haemostasis					81:91	topical haemostasis	73:91	topical haemostasis	73:91	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	3	4	theme	wound	514:518	arg1	RapidClot					530:538	RapidClot	530:538	RapidClot	530:538	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	3	4	theme	wound	514:518	arg1	dressing					520:527	new wound dressing	510:527	new wound dressing	510:527	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	0	5	theme	wound	97:101	arg1	healing					103:109	wound healing	97:109	wound healing	97:109	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	3	6	theme	haemolytic	691:700	arg1	activity					702:709	haemolytic activity	691:709	haemolytic activity	691:709	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	2	7	theme	better	358:363	arg1	activity					377:384	better haemostatic activity	358:384	better haemostatic activity	358:384	However, there are still urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products.
31845534	2	8	theme	urgent	298:303	arg1	demands					305:311	urgent demands	298:311	urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products	298:447	However, there are still urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products.
31845534	7	9	theme	haemorrhage	1535:1545	arg1	ability					1555:1561	haemorrhage control ability	1535:1561	haemorrhage control ability	1535:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	3	10	theme	cell	726:729	arg1	proliferation					731:743	cell proliferation	726:743	cell proliferation	726:743	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	3	11	theme	new	510:512	arg1	RapidClot					530:538	RapidClot	530:538	RapidClot	530:538	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	3	11	theme	new	510:512	arg1	dressing					520:527	new wound dressing	510:527	new wound dressing	510:527	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	1	12	theme	haemostatic	123:133	arg1	agents					135:140	haemostatic agents	123:140	haemostatic agents	123:140	The use of haemostatic agents can provide life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions.
31845534	6	13	theme	cell	1331:1334	arg1	proliferation					1336:1348	improved cell proliferation	1322:1348	improved cell proliferation	1322:1348	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	5	14	theme	hyaluronidase	1095:1107	arg1	presence					1083:1090	the presence	1079:1090	the presence of hyaluronidase and lysozyme	1079:1120	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	4	15	theme	blood	856:860	arg1	time					871:874	blood clotting time	856:874	blood clotting time (132.7 seconds)	856:890	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	15	theme	blood	856:860	arg1	seconds					883:889	132.7 seconds	877:889	132.7 seconds	877:889	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	3	16	theme	present	457:463	arg1	study					465:469	the present study	453:469	the present study	453:469	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	6	17	theme	improved	1322:1329	arg1	proliferation					1336:1348	improved cell proliferation	1322:1348	improved cell proliferation	1322:1348	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	4	18	theme	shorter	848:854	arg1	Celox					943:947	shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox	848:947	shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox	848:947	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	5	19	theme	lysozyme	1113:1120	arg1	presence					1083:1090	the presence	1079:1090	the presence of hyaluronidase and lysozyme	1079:1120	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	2	20	theme	novel	317:321	arg1	materials					335:343	novel haemostatic materials	317:343	novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products	317:447	However, there are still urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products.
31845534	3	21	dep	in	599:600	arg1	vitro					602:606	vitro	602:606	vitro	602:606	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	0	22	theme	In	0:1	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.	0:110	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	4	23	theme	clotting	862:869	arg1	time					871:874	blood clotting time	856:874	blood clotting time (132.7 seconds)	856:890	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	23	theme	clotting	862:869	arg1	seconds					883:889	132.7 seconds	877:889	132.7 seconds	877:889	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	6	24	theme	cell	1260:1263	arg1	viability					1265:1273	cell viability	1260:1273	cell viability	1260:1273	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	4	25	theme	better	811:816	arg1	capacity					835:842	better water adsorption capacity	811:842	better water adsorption capacity	811:842	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	25	theme	better	811:816	arg1	seconds					956:962	378.7 seconds	950:962	378.7 seconds	950:962	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	7	26	theme	control	1547:1553	arg1	ability					1555:1561	haemorrhage control ability	1535:1561	haemorrhage control ability	1535:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	3	27	theme	uncontrolled	554:565	arg1	haemorrhage					567:577	uncontrolled haemorrhage	554:577	uncontrolled haemorrhage	554:577	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	4	28	theme	adsorption	824:833	arg1	capacity					835:842	better water adsorption capacity	811:842	better water adsorption capacity	811:842	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	28	theme	adsorption	824:833	arg1	seconds					956:962	378.7 seconds	950:962	378.7 seconds	950:962	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	29	dep	shorter	848:854	arg1	time					871:874	blood clotting time	856:874	blood clotting time (132.7 seconds)	856:890	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	29	dep	shorter	848:854	arg1	seconds					883:889	132.7 seconds	877:889	132.7 seconds	877:889	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	30	theme	haemostatic	924:934	arg1	agents					936:941	two commercially available haemostatic agents	897:941	two commercially available haemostatic agents	897:941	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	5	31	theme	degradability	1062:1074	arg1	level					1053:1057	a similar level	1043:1057	a similar level of degradability in the presence of hyaluronidase and lysozyme	1043:1120	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	1	32	theme	life-saving	154:164	arg1	treatment					166:174	life-saving treatment	154:174	life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions	154:270	The use of haemostatic agents can provide life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions.
31845534	7	33	with	dressing	1521:1528	arg1	ability					1555:1561	haemorrhage control ability	1535:1561	haemorrhage control ability	1535:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	3	34	theme	dressing	520:527	arg1	feasibility					495:505	the feasibility	491:505	the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration	491:758	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	0	35	theme	hyaluronic	27:36	arg1	powder					62:67	the hyaluronic acid/alginate composite powder	23:67	the hyaluronic acid/alginate composite powder	23:67	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	6	36	theme	T3	1306:1307	arg1	fibroblasts					1309:1319	NIH/3 T3 fibroblasts	1300:1319	NIH/3 T3 fibroblasts	1300:1319	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	3	37	theme	clotting	653:660	arg1	time					662:665	clotting time	653:665	clotting time	653:665	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	3	38	theme	in	599:600	arg1	assessments					608:618	in vitro assessments	599:618	in vitro assessments	599:618	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	6	39	theme	NIH/3	1300:1304	arg1	fibroblasts					1309:1319	NIH/3 T3 fibroblasts	1300:1319	NIH/3 T3 fibroblasts	1300:1319	Although both Celox and RapidClot revealed a similar level in cell viability (above than 90%) against NIH/3 T3 fibroblasts, improved cell proliferation and migration could be obtained in RapidClot.
31845534	3	40	theme	swelling	637:644	arg1	ratio					646:650	the swelling ratio	633:650	the swelling ratio	633:650	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	4	41	theme	water	818:822	arg1	capacity					835:842	better water adsorption capacity	811:842	better water adsorption capacity	811:842	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	4	41	theme	water	818:822	arg1	seconds					956:962	378.7 seconds	950:962	378.7 seconds	950:962	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	0	42	theme	composite	52:60	arg1	powder					62:67	the hyaluronic acid/alginate composite powder	23:67	the hyaluronic acid/alginate composite powder	23:67	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	7	43	theme	healing	1513:1519	arg1	dressing					1521:1528	an efficient healing dressing	1500:1528	an efficient healing dressing with haemorrhage control ability	1500:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	5	44	theme	WoundSeal	1174:1182	arg1	degradation					1159:1169	negligible degradation	1148:1169	negligible degradation of WoundSeal	1148:1182	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	0	45	theme	acid/alginate	38:50	arg1	powder					62:67	the hyaluronic acid/alginate composite powder	23:67	the hyaluronic acid/alginate composite powder	23:67	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	5	46	theme	negligible	1148:1157	arg1	degradation					1159:1169	negligible degradation	1148:1169	negligible degradation of WoundSeal	1148:1182	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	3	47	theme	enzymatic	668:676	arg1	degradation					678:688	enzymatic degradation	668:688	enzymatic degradation	668:688	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	3	48	from	aim	475:477	arg1	study					465:469	the present study	453:469	the present study	453:469	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	7	49	theme	efficient	1503:1511	arg1	dressing					1521:1528	an efficient healing dressing	1500:1528	an efficient healing dressing with haemorrhage control ability	1500:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	4	50	theme	available	914:922	arg1	agents					936:941	two commercially available haemostatic agents	897:941	two commercially available haemostatic agents	897:941	The results indicated that the RapidClot revealed better water adsorption capacity and shorter blood clotting time (132.7 seconds) than two commercially available haemostatic agents Celox (378.7 seconds) and WoundSeal (705.3 seconds).
31845534	5	51	theme	similar	1045:1051	arg1	level					1053:1057	a similar level	1043:1057	a similar level of degradability in the presence of hyaluronidase and lysozyme	1043:1120	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	5	52	theme	RapidClot	1014:1022	arg1	dressing					1024:1031	the RapidClot dressing	1010:1031	the RapidClot dressing	1010:1031	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	2	53	theme	haemostatic	323:333	arg1	materials					335:343	novel haemostatic materials	317:343	novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products	317:447	However, there are still urgent demands for novel haemostatic materials that exhibit better haemostatic activity, biocompatibility, and biodegradability than existing products.
31845534	5	54	from	level	1053:1057	arg1	presence					1083:1090	the presence	1079:1090	the presence of hyaluronidase and lysozyme	1079:1120	Additionally, the RapidClot dressing exhibited a similar level of degradability in the presence of hyaluronidase and lysozyme as that of Celox, whereas negligible degradation of WoundSeal was obtained.
31845534	3	55	theme	assessments	608:618	arg1	series					589:594	a series	587:594	a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration	587:758	In the present study, we aim to evaluate the feasibility of new wound dressing, RapidClot, for treating uncontrolled haemorrhage through a series of in vitro assessments to determine the swelling ratio, clotting time, enzymatic degradation, haemolytic activity, cytotoxicity, cell proliferation, and migration.
31845534	1	56	theme	agents	135:140	arg1	use					116:118	The use	112:118	The use of haemostatic agents	112:140	The use of haemostatic agents can provide life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions.
31845534	1	57	theme	prehospital	230:240	arg1	conditions					261:270	both prehospital and intraoperative conditions	225:270	both prehospital and intraoperative conditions	225:270	The use of haemostatic agents can provide life-saving treatment for patients who suffer from massive bleeding in both prehospital and intraoperative conditions.
31845534	0	58	theme	powder	62:67	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.	0:110	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	7	59	theme	great	1469:1473	arg1	potential					1475:1483	a great potential	1467:1483	a great potential for serving as an efficient healing dressing with haemorrhage control ability	1467:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	0	60	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro evaluation of the hyaluronic acid/alginate composite powder for topical haemostasis and wound healing.
31845534	7	61	contain	possess	1459:1465	arg1	RapidClot					1443:1451	RapidClot	1443:1451	RapidClot	1443:1451	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31845534	7	61	contain	possess	1459:1465	arg2	potential					1475:1483	a great potential	1467:1483	a great potential for serving as an efficient healing dressing with haemorrhage control ability	1467:1561	Taking together, our results demonstrated that RapidClot could possess a great potential for serving as an efficient healing dressing with haemorrhage control ability.
31276724	7	0	theme	liver	1396:1400	arg1	injury					1402:1407	alcohol-induced liver injury	1380:1407	alcohol-induced liver injury in mice	1380:1415	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	3	1	theme	submerged	514:522	arg1	culture					524:530	submerged culture	514:530	submerged culture	514:530	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	6	2	attach	linked	1231:1236	arg2	branches					1222:1229	secondary branches	1212:1229	secondary branches	1212:1229	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	2	attach	linked	1231:1236	arg1	position					1249:1256	the O-6 position	1241:1256	the O-6 position of β-Glcp of the major branch	1241:1286	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	4	3	theme	glucuronic	589:598	arg1	acid					600:603	glucuronic acid	589:603	glucuronic acid	589:603	PSP-1b1 consists of fucose, galactose, xylose, mannose, glucuronic acid and glucose at a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00.
31276724	5	4	theme	Structural	677:686	arg1	features					688:695	Structural features	677:695	Structural features	677:695	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	6	5	theme	PSP-1b1	861:867	arg1	backbone					869:876	The PSP-1b1 backbone	857:876	The PSP-1b1 backbone	857:876	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	1	6	theme	liver	282:286	arg1	diseases					288:295	liver diseases	282:295	liver diseases	282:295	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	7	7	theme	hepatoprotective	1347:1362	arg1	effects					1364:1370	hepatoprotective effects	1347:1370	hepatoprotective effects against alcohol-induced liver injury in mice	1347:1415	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	3	8	with	polysaccharide	423:436	arg1	weight					465:470	a molecular weight	453:470	a molecular weight of 21.7 kDa	453:482	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	4	9	theme	0.16:0.60:0.02:0.55:0.04:1.00	646:674	arg1	ratio					637:641	a relative molar ratio	620:641	a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00	620:674	PSP-1b1 consists of fucose, galactose, xylose, mannose, glucuronic acid and glucose at a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00.
31276724	7	10	with	Treatment	1289:1297	arg1	80					1313:1314	80	1313:1314	80	1313:1314	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	7	10	with	Treatment	1289:1297	arg1	PSK-1b1					1304:1310	PSK-1b1	1304:1310	PSK-1b1 (80 and 160 mg/kg/day)	1304:1333	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	7	10	with	Treatment	1289:1297	arg1	160 mg/kg/day					1320:1332	160 mg/kg/day	1320:1332	160 mg/kg/day	1320:1332	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	6	11	theme	T-α-Glcp	1137:1144	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	4	12	theme	relative	622:629	arg1	ratio					637:641	a relative molar ratio	620:641	a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00	620:674	PSP-1b1 consists of fucose, galactose, xylose, mannose, glucuronic acid and glucose at a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00.
31276724	5	13	theme	chromatography-mass	738:756	arg1	spectrometry					758:769	gas chromatography-mass spectrometry	734:769	gas chromatography-mass spectrometry	734:769	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	5	14	dep	Fourier	772:778	arg1	transform					780:788	transform	780:788	transform infrared spectroscopy and nuclear magnetic resonance spectroscopy	780:854	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	2	15	dep	composition	311:321	arg1	the					307:309	the	307:309	the	307:309	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	6	16	from	T-α-Glcp	1137:1144	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	17	theme	chain	1201:1205	arg1	α-Manp					1182:1187	α-Manp	1182:1187	α-Manp of the main chain	1182:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	18	from	versicolor	103:112	arg1	activity					60:67	anti-alcoholic liver injury activity	32:67	anti-alcoholic liver injury activity	32:67	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	0	18	from	versicolor	103:112	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	0	18	from	versicolor	103:112	arg1	polysaccharide					74:87	a polysaccharide	72:87	a polysaccharide from Coriolus versicolor mycelia	72:120	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	19	from	T-α-Galp	1150:1157	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	20	theme	major	1275:1279	arg1	branch					1281:1286	the major branch	1271:1286	the major branch	1271:1286	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	1	21	from	versicolor	186:195	arg1	immunotherapy					243:255	an adjunctive immunotherapy	229:255	an adjunctive immunotherapy for treating cancers and liver diseases	229:295	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	21	from	versicolor	186:195	arg1	PSP					144:146	PSP	144:146	PSP	144:146	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	21	from	versicolor	186:195	arg1	Polysaccharopeptide					123:141	Polysaccharopeptide	123:141	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor	123:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	22	theme	medicinal	158:166	arg1	versicolor					186:195	the medicinal mushroom Coriolus versicolor	154:195	the medicinal mushroom Coriolus versicolor	154:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	2	23	from	composition	311:321	arg1	PSP					364:366	PSP	364:366	PSP	364:366	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	6	24	from	α-1,6-Galp	1113:1122	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	5	25	theme	nuclear	816:822	arg1	resonance					833:841	nuclear magnetic resonance	816:841	nuclear magnetic resonance spectroscopy	816:854	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	1	26	theme	Coriolus	177:184	arg1	versicolor					186:195	the medicinal mushroom Coriolus versicolor	154:195	the medicinal mushroom Coriolus versicolor	154:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	2	27	from	structure	327:335	arg1	PSP					364:366	PSP	364:366	PSP	364:366	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	6	28	from	α-1,3-Fucp	1125:1134	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	29	from	activity	60:67	arg1	versicolor					103:112	Coriolus versicolor mycelia	94:120	Coriolus versicolor mycelia	94:120	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	30	from	β-1,3,6-Glcp	1087:1098	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	31	theme	O-6	1245:1247	arg1	position					1249:1256	the O-6 position	1241:1256	the O-6 position of β-Glcp of the major branch	1241:1286	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	32	theme	injury	53:58	arg1	activity					60:67	anti-alcoholic liver injury activity	32:67	anti-alcoholic liver injury activity	32:67	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	0	33	dep	versicolor	103:112	arg1	mycelia					114:120	Coriolus versicolor mycelia	94:120	Coriolus versicolor mycelia	94:120	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	0	34	theme	polysaccharide	74:87	arg1	activity					60:67	anti-alcoholic liver injury activity	32:67	anti-alcoholic liver injury activity	32:67	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	0	34	theme	polysaccharide	74:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	35	from	α-1,3-Manp	1101:1110	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	36	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	37	from	α-1,6-Manp	1063:1072	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	38	theme	-α-Galp-	908:915	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	39	from	β-1,6-Glcp	1075:1084	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	3	40	theme	molecular	455:463	arg1	weight					465:470	a molecular weight	453:470	a molecular weight of 21.7 kDa	453:482	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	0	41	theme	liver	47:51	arg1	activity					60:67	anti-alcoholic liver injury activity	32:67	anti-alcoholic liver injury activity	32:67	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	5	42	theme	resonance	833:841	arg1	spectroscopy					843:854	nuclear magnetic resonance spectroscopy	816:854	nuclear magnetic resonance spectroscopy	816:854	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	6	43	theme	1 → 4	902:906	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	3	44	theme	versicolor	492:501	arg1	mycelia					503:509	C. versicolor mycelia	489:509	C. versicolor mycelia in submerged culture	489:530	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	6	45	theme	-α-Galp-	893:900	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	46	from	branches	1051:1058	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	2	47	theme	bioactive	340:348	arg1	components					350:359	bioactive components	340:359	bioactive components	340:359	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	6	48	theme	→4	890:891	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	49	theme	-α-Galp-	938:945	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	50	theme	main	1196:1199	arg1	chain					1201:1205	the main chain	1192:1205	the main chain	1192:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	51	theme	-α-Manp-	923:930	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	52	theme	α-Manp	1182:1187	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	53	theme	α-1,3-Manp	1101:1110	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	54	theme	β-1,3,6-Glcp	1087:1098	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	55	theme	O-6	1166:1168	arg1	position					1170:1177	the O-6 position	1162:1177	the O-6 position of α-Manp of the main chain	1162:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	56	theme	-α-Galp-	968:975	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	57	theme	β-1,6-Glcp	1075:1084	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	3	58	from	mycelia	503:509	arg1	culture					524:530	submerged culture	514:530	submerged culture	514:530	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	6	59	theme	1 → 4	962:966	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	60	theme	α-1,6-Manp	1063:1072	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	5	61	theme	gas	734:736	arg1	spectrometry					758:769	gas chromatography-mass spectrometry	734:769	gas chromatography-mass spectrometry	734:769	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	6	62	theme	-α-Manp-	953:960	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	63	theme	T-α-Galp	1150:1157	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	4	64	theme	molar	631:635	arg1	ratio					637:641	a relative molar ratio	620:641	a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00	620:674	PSP-1b1 consists of fucose, galactose, xylose, mannose, glucuronic acid and glucose at a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00.
31276724	6	65	theme	secondary	1212:1220	arg1	branches					1222:1229	secondary branches	1212:1229	secondary branches	1212:1229	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	5	66	dep	transform	780:788	arg1	infrared					790:797	infrared	790:797	transform infrared spectroscopy and nuclear magnetic resonance spectroscopy	780:854	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	1	67	used	used	213:216	arg2	Polysaccharopeptide					123:141	Polysaccharopeptide	123:141	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor	123:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	67	used	used	213:216	arg2	PSP					144:146	PSP	144:146	PSP	144:146	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	67	used	used	213:216	arg2	immunotherapy					243:255	an adjunctive immunotherapy	229:255	an adjunctive immunotherapy for treating cancers and liver diseases	229:295	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	6	68	from	position	1170:1177	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	69	theme	-α-Manp-	998:1005	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	70	theme	α-1,3-Fucp	1125:1134	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	71	theme	branch	1281:1286	arg1	β-Glcp					1261:1266	β-Glcp	1261:1266	β-Glcp of the major branch	1261:1286	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	72	theme	1 → 2	992:996	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	73	theme	α-1,6-Galp	1113:1122	arg1	branches					1051:1058	branches	1051:1058	branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain	1051:1205	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	74	theme	Coriolus	94:101	arg1	versicolor					103:112	Coriolus versicolor mycelia	94:120	Coriolus versicolor mycelia	94:120	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	1	75	theme	mushroom	168:175	arg1	versicolor					186:195	the medicinal mushroom Coriolus versicolor	154:195	the medicinal mushroom Coriolus versicolor	154:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	6	76	theme	-α-Galp-	983:990	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	77	from	characterization	11:26	arg1	versicolor					103:112	Coriolus versicolor mycelia	94:120	Coriolus versicolor mycelia	94:120	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	78	theme	β-Glcp	1261:1266	arg1	position					1249:1256	the O-6 position	1241:1256	the O-6 position of β-Glcp of the major branch	1241:1286	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	79	theme	1 → 4	977:981	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	6	80	theme	-α-Manp-	1028:1035	arg1	→					1043:1043	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	0	81	theme	anti-alcoholic	32:45	arg1	activity					60:67	anti-alcoholic liver injury activity	32:67	anti-alcoholic liver injury activity	32:67	Structural characterization and anti-alcoholic liver injury activity of a polysaccharide from Coriolus versicolor mycelia.
31276724	6	82	theme	-α-Galp-	1013:1020	arg1	-α-Manp-					1028:1035	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)	890:1042	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	3	83	theme	21.7 kDa	475:482	arg1	weight					465:470	a molecular weight	453:470	a molecular weight of 21.7 kDa	453:482	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	5	84	theme	magnetic	824:831	arg1	resonance					833:841	nuclear magnetic resonance	816:841	nuclear magnetic resonance spectroscopy	816:854	Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
31276724	6	85	theme	1 → 4	1007:1011	arg1	-α-Galp-					1013:1020	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)	890:1027	→4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→	890:1043	The PSP-1b1 backbone consists of →4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)-α-Galp-(1 → 2)-α-Manp-(1 → 4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch.
31276724	7	86	theme	oxidative	1429:1437	arg1	stress					1439:1444	oxidative stress	1429:1444	oxidative stress	1429:1444	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	3	87	theme	hepatoprotective	406:421	arg1	PSP-1b1					439:445	PSP-1b1	439:445	PSP-1b1	439:445	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	3	87	theme	hepatoprotective	406:421	arg1	polysaccharide					423:436	a hepatoprotective polysaccharide	404:436	a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa	404:482	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	7	88	theme	alcohol-induced	1380:1394	arg1	injury					1402:1407	alcohol-induced liver injury	1380:1407	alcohol-induced liver injury in mice	1380:1415	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31276724	2	89	theme	components	350:359	arg1	composition					311:321	composition	311:321	composition	311:321	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	2	89	theme	components	350:359	arg1	structure					327:335	structure	327:335	structure	327:335	However, the composition and structure of bioactive components in PSP remain elusive.
31276724	3	90	theme	C.	489:490	arg1	mycelia					503:509	C. versicolor mycelia	489:509	C. versicolor mycelia in submerged culture	489:530	Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture.
31276724	1	91	theme	adjunctive	232:241	arg1	immunotherapy					243:255	an adjunctive immunotherapy	229:255	an adjunctive immunotherapy for treating cancers and liver diseases	229:295	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	1	91	theme	adjunctive	232:241	arg1	Polysaccharopeptide					123:141	Polysaccharopeptide	123:141	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor	123:195	Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases.
31276724	7	92	from	injury	1402:1407	arg1	mice					1412:1415	mice	1412:1415	mice	1412:1415	Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
31755197	5	0	dep	ascomycete	881:890	arg1	TrCel7B					912:918	TrCel7B	912:918	TrCel7B	912:918	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	3	1	theme	few	588:590	arg1	structures					592:601	only few structures	583:601	only few structures	583:601	While substantial biochemical and structural data are available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far.
31755197	2	2	theme	Activity	291:298	arg1	strategy					364:371	a promising strategy	352:371	a promising strategy to alleviate the industrial costs	352:405	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	2	2	theme	Activity	291:298	arg1	improvement					300:310	Activity improvement	291:310	Activity improvement of these enzymes via rational design	291:347	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	4	3	from	characterization	684:699	arg1	ReCel7B					788:794	ReCel7B	788:794	ReCel7B	788:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	3	from	characterization	684:699	arg1	Rasamsonia emersonii					766:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	2	4	theme	rational	333:340	arg1	design					342:347	rational design	333:347	rational design	333:347	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	11	5	from	differences	1817:1827	arg1	endoglucanases					1909:1922	GH7 endoglucanases	1905:1922	GH7 endoglucanases	1905:1922	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	11	5	from	differences	1817:1827	arg1	stability					1844:1852	temperature stability	1832:1852	temperature stability	1832:1852	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	11	5	from	differences	1817:1827	arg1	binding					1865:1871	binding	1865:1871	binding	1865:1871	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	11	5	from	differences	1817:1827	arg1	turnover					1855:1862	turnover	1855:1862	turnover	1855:1862	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	11	5	from	differences	1817:1827	arg1	accessibility					1888:1900	cellulose accessibility	1878:1900	cellulose accessibility	1878:1900	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	4	6	theme	crystal	650:656	arg1	structure					658:666	a new crystal structure	644:666	a new crystal structure	644:666	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	10	7	theme	increased	1624:1632	arg1	number					1634:1639	An increased number	1621:1639	An increased number of glycosylations	1621:1657	An increased number of glycosylations were identified in ReCel7B, and we propose a stabilizing mechanism for one of the glycans.
31755197	2	8	theme	enzymes	321:327	arg1	strategy					364:371	a promising strategy	352:371	a promising strategy to alleviate the industrial costs	352:405	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	2	8	theme	enzymes	321:327	arg1	improvement					300:310	Activity improvement	291:310	Activity improvement of these enzymes via rational design	291:347	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	1	9	theme	main	202:205	arg1	cellulases					143:152	Thermostable cellulases	130:152	Thermostable cellulases from glycoside hydrolase family 7 (GH7)	130:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	9	theme	main	202:205	arg1	components					207:216	the main components	198:216	the main components of enzymatic mixtures for industrial saccharification of lignocellulose	198:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	8	10	theme	crystal	1323:1329	arg1	structure					1331:1339	The crystal structure	1319:1339	The crystal structure of ReCel7B	1319:1350	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	11	11	theme	cellulose	1878:1886	arg1	accessibility					1888:1900	cellulose accessibility	1878:1900	cellulose accessibility	1878:1900	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	6	12	theme	temperature	1009:1019	arg1	optimum					1021:1027	a temperature optimum	1007:1027	a temperature optimum of 80 °C	1007:1036	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	4	13	theme	ascomycete	755:764	arg1	ReCel7B					788:794	ReCel7B	788:794	ReCel7B	788:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	13	theme	ascomycete	755:764	arg1	Rasamsonia emersonii					766:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	1	14	theme	Thermostable	130:141	arg1	cellulases					143:152	Thermostable cellulases	130:152	Thermostable cellulases from glycoside hydrolase family 7 (GH7)	130:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	14	theme	Thermostable	130:141	arg1	components					207:216	the main components	198:216	the main components of enzymatic mixtures for industrial saccharification of lignocellulose	198:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	13	15	dep	ENZYMES	2033:2039	arg1	ReCel7B					2042:2048	ReCel7B	2042:2048	ReCel7B	2042:2048	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	8	16	theme	ReCel7B	1344:1350	arg1	structure					1331:1339	The crystal structure	1319:1339	The crystal structure of ReCel7B	1319:1350	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	9	17	theme	loops	1543:1547	arg1	number					1522:1527	a number	1520:1527	a number of peripheral loops	1520:1547	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	4	18	theme	endoglucanase	719:731	arg1	structure					658:666	a new crystal structure	644:666	a new crystal structure	644:666	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	18	theme	endoglucanase	719:731	arg1	characterization					684:699	biochemical characterization	672:699	biochemical characterization	672:699	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	9	19	theme	Structural	1452:1461	arg1	analyses					1463:1470	Structural analyses	1452:1470	Structural analyses	1452:1470	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	5	20	theme	additional	956:965	arg1	CBM					996:998	CBM	996:998	CBM	996:998	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	5	20	theme	additional	956:965	arg1	module					988:993	an additional carbohydrate-binding module	953:993	an additional carbohydrate-binding module (CBM)	953:999	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	6	21	theme	differences	1069:1079	arg1	number					1059:1064	a number	1057:1064	a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B	1057:1160	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	13	22	from	Trichoderma reesei	2278:2295	arg1	cellobiohydrolase					2241:2257	cellobiohydrolase	2241:2257	cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei	2241:2295	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	22	from	Trichoderma reesei	2278:2295	arg1	EC3.2.1.176					2260:2270	EC3.2.1.176	2260:2270	EC3.2.1.176	2260:2270	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	8	23	theme	2.48 Å	1370:1375	arg1	resolution					1377:1386	2.48 Å resolution	1370:1386	2.48 Å resolution	1370:1386	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	11	24	theme	temperature	1832:1842	arg1	stability					1844:1852	temperature stability	1832:1852	temperature stability	1832:1852	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	0	25	theme	endoglucanase	78:90	arg1	characterization					27:42	Structural and biochemical characterization	0:42	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.	0:128	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	12	26	theme	Protein	1973:1979	arg1	database					1991:1998	RCSB Protein Data Bank database	1968:1998	RCSB Protein Data Bank database under the accession number 6SU8	1968:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	1	27	from	family	179:184	arg1	cellulases					143:152	Thermostable cellulases	130:152	Thermostable cellulases from glycoside hydrolase family 7 (GH7)	130:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	27	from	family	179:184	arg1	components					207:216	the main components	198:216	the main components of enzymatic mixtures for industrial saccharification of lignocellulose	198:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	5	28	from	ascomycete	881:890	arg1	endoglucanase					841:853	the homologous endoglucanase	826:853	the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM)	826:999	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	12	29	theme	Bank	1986:1989	arg1	database					1991:1998	RCSB Protein Data Bank database	1968:1998	RCSB Protein Data Bank database under the accession number 6SU8	1968:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	3	30	theme	substantial	461:471	arg1	data					500:503	substantial biochemical and structural data	461:503	substantial biochemical and structural data	461:503	While substantial biochemical and structural data are available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far.
31755197	0	31	theme	Rasamsonia	108:117	arg1	emersonii					119:127	the fungus Rasamsonia emersonii	97:127	the fungus Rasamsonia emersonii	97:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	8	32	theme	Rfree	1404:1408	arg1	factors					1410:1416	Rfree factors	1404:1416	Rfree factors	1404:1416	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	0	33	theme	Structural	0:9	arg1	characterization					27:42	Structural and biochemical characterization	0:42	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.	0:128	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	0	34	theme	biochemical	15:25	arg1	characterization					27:42	Structural and biochemical characterization	0:42	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.	0:128	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	endoglucanase					2051:2063	endoglucanase (EC3.2.1.4)	2051:2075	endoglucanase (EC3.2.1.4) from Rasamsonia emersonii	2051:2101	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	ReCel7A					2104:2110	ReCel7A	2104:2110	ReCel7A	2104:2110	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	cellobiohydrolase					2241:2257	cellobiohydrolase	2241:2257	cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei	2241:2295	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	cellobiohydrolase					2113:2129	cellobiohydrolase	2113:2129	cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii	2113:2169	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	EC3.2.1.176					2260:2270	EC3.2.1.176	2260:2270	EC3.2.1.176	2260:2270	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	TrCel7B					2172:2178	TrCel7B	2172:2178	TrCel7B	2172:2178	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	EC3.2.1.176					2132:2142	EC3.2.1.176	2132:2142	EC3.2.1.176	2132:2142	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	TrCel7A					2232:2238	TrCel7A	2232:2238	TrCel7A	2232:2238	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	35	dep	ReCel7B	2042:2048	arg1	endoglucanase					2181:2193	endoglucanase (EC3.2.1.4)	2181:2205	endoglucanase (EC3.2.1.4) from Trichoderma reesei	2181:2229	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	7	36	theme	CBM	1240:1242	arg1	variant					1215:1221	a chimeric variant	1204:1221	a chimeric variant of ReCel7B and a CBM	1204:1242	We improved both binding and kinetics in a chimeric variant of ReCel7B and a CBM, while we observe the opposite effect when the CBM was removed in TrCel7B.
31755197	10	37	theme	stabilizing	1704:1714	arg1	mechanism					1716:1724	a stabilizing mechanism	1702:1724	a stabilizing mechanism for one of the glycans	1702:1747	An increased number of glycosylations were identified in ReCel7B, and we propose a stabilizing mechanism for one of the glycans.
31755197	12	38	theme	Structural	1935:1944	arg1	data					1946:1949	Structural data	1935:1949	Structural data	1935:1949	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	10	39	theme	glycans	1741:1747	arg1	one					1730:1732	one	1730:1732	one	1730:1732	An increased number of glycosylations were identified in ReCel7B, and we propose a stabilizing mechanism for one of the glycans.
31755197	10	39	theme	glycans	1741:1747	arg1	glycans					1741:1747	the glycans	1737:1747	the glycans	1737:1747	An increased number of glycosylations were identified in ReCel7B, and we propose a stabilizing mechanism for one of the glycans.
31755197	0	40	theme	family	49:54	arg1	endoglucanase					78:90	a family 7 highly thermostable endoglucanase	47:90	a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii	47:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	4	41	from	Rasamsonia emersonii	766:785	arg1	structure					658:666	a new crystal structure	644:666	a new crystal structure	644:666	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	41	from	Rasamsonia emersonii	766:785	arg1	characterization					684:699	biochemical characterization	672:699	biochemical characterization	672:699	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	41	from	Rasamsonia emersonii	766:785	arg1	endoglucanase					719:731	a thermostable endoglucanase	704:731	a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B	704:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	1	42	theme	enzymatic	221:229	arg1	mixtures					231:238	enzymatic mixtures	221:238	enzymatic mixtures	221:238	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	43	theme	hydrolase	169:177	arg1	family					179:184	glycoside hydrolase family 7	159:186	glycoside hydrolase family 7 (GH7)	159:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	43	theme	hydrolase	169:177	arg1	GH7					189:191	GH7	189:191	GH7	189:191	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	11	44	theme	Global	1750:1755	arg1	interpretations					1776:1790	Global structure-function interpretations	1750:1790	Global structure-function interpretations of ReCel7B	1750:1801	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	9	45	dep	increased	1498:1506	arg1	compared					1549:1556	compared	1549:1556	compared to TrCel7B and fewer aromatics in the substrate-binding cleft	1549:1618	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	2	46	theme	industrial	390:399	arg1	costs					401:405	the industrial costs	386:405	the industrial costs	386:405	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	13	47	from	Rasamsonia emersonii	2150:2169	arg1	EC3.2.1.176					2132:2142	EC3.2.1.176	2132:2142	EC3.2.1.176	2132:2142	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	13	47	from	Rasamsonia emersonii	2150:2169	arg1	cellobiohydrolase					2113:2129	cellobiohydrolase	2113:2129	cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii	2113:2169	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	9	48	from	TrCel7B	1561:1567	arg1	cleft					1614:1618	the substrate-binding cleft	1592:1618	the substrate-binding cleft	1592:1618	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	1	49	theme	lignocellulose	275:288	arg1	saccharification					255:270	industrial saccharification	244:270	industrial saccharification of lignocellulose	244:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	3	50	theme	GH7	523:525	arg1	cellobiohydrolases					527:544	GH7 cellobiohydrolases	523:544	GH7 cellobiohydrolases	523:544	While substantial biochemical and structural data are available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far.
31755197	7	51	theme	chimeric	1206:1213	arg1	variant					1215:1221	a chimeric variant	1204:1221	a chimeric variant of ReCel7B and a CBM	1204:1242	We improved both binding and kinetics in a chimeric variant of ReCel7B and a CBM, while we observe the opposite effect when the CBM was removed in TrCel7B.
31755197	4	52	theme	biochemical	672:682	arg1	characterization					684:699	biochemical characterization	672:699	biochemical characterization	672:699	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	10	53	theme	glycosylations	1644:1657	arg1	number					1634:1639	An increased number	1621:1639	An increased number of glycosylations	1621:1657	An increased number of glycosylations were identified in ReCel7B, and we propose a stabilizing mechanism for one of the glycans.
31755197	7	54	theme	opposite	1266:1273	arg1	effect					1275:1280	the opposite effect	1262:1280	the opposite effect	1262:1280	We improved both binding and kinetics in a chimeric variant of ReCel7B and a CBM, while we observe the opposite effect when the CBM was removed in TrCel7B.
31755197	6	55	from	ability	1097:1103	arg1	number					1059:1064	a number	1057:1064	a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B	1057:1160	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	4	56	from	structure	658:666	arg1	ReCel7B					788:794	ReCel7B	788:794	ReCel7B	788:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	56	from	structure	658:666	arg1	Rasamsonia emersonii					766:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	6	57	theme	80 °C	1032:1036	arg1	optimum					1021:1027	a temperature optimum	1007:1027	a temperature optimum of 80 °C	1007:1036	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	4	58	theme	new	646:648	arg1	structure					658:666	a new crystal structure	644:666	a new crystal structure	644:666	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	12	59	theme	accession	2010:2018	arg1	number					2020:2025	the accession number 6SU8	2006:2030	the accession number 6SU8	2006:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	0	60	from	emersonii	119:127	arg1	endoglucanase					78:90	a family 7 highly thermostable endoglucanase	47:90	a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii	47:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	0	60	from	emersonii	119:127	arg1	characterization					27:42	Structural and biochemical characterization	0:42	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.	0:128	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	4	61	theme	thermophilic	742:753	arg1	ReCel7B					788:794	ReCel7B	788:794	ReCel7B	788:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	4	61	theme	thermophilic	742:753	arg1	Rasamsonia emersonii					766:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	the thermophilic ascomycete Rasamsonia emersonii	738:785	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	9	62	theme	peripheral	1532:1541	arg1	loops					1543:1547	peripheral loops	1532:1547	peripheral loops	1532:1547	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	12	63	from	database	1991:1998	arg1	available					1955:1963	available	1955:1963	available	1955:1963	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	12	63	from	database	1991:1998	arg1	DATABASE					1925:1932	DATABASE	1925:1932	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.	1925:2031	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	0	64	theme	thermostable	65:76	arg1	endoglucanase					78:90	a family 7 highly thermostable endoglucanase	47:90	a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii	47:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	1	65	theme	glycoside	159:167	arg1	family					179:184	glycoside hydrolase family 7	159:186	glycoside hydrolase family 7 (GH7)	159:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	65	theme	glycoside	159:167	arg1	GH7					189:191	GH7	189:191	GH7	189:191	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	0	66	from	characterization	27:42	arg1	emersonii					119:127	the fungus Rasamsonia emersonii	97:127	the fungus Rasamsonia emersonii	97:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	4	67	theme	thermostable	706:717	arg1	endoglucanase					719:731	a thermostable endoglucanase	704:731	a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B	704:794	Here, we report a new crystal structure and biochemical characterization of a thermostable endoglucanase from the thermophilic ascomycete Rasamsonia emersonii, ReCel7B.
31755197	11	68	theme	ReCel7B	1795:1801	arg1	interpretations					1776:1790	Global structure-function interpretations	1750:1790	Global structure-function interpretations of ReCel7B	1750:1801	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	12	69	theme	Data	1981:1984	arg1	database					1991:1998	RCSB Protein Data Bank database	1968:1998	RCSB Protein Data Bank database under the accession number 6SU8	1968:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	5	70	theme	carbohydrate-binding	967:986	arg1	CBM					996:998	CBM	996:998	CBM	996:998	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	5	70	theme	carbohydrate-binding	967:986	arg1	module					988:993	an additional carbohydrate-binding module	953:993	an additional carbohydrate-binding module (CBM)	953:999	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	6	71	from	differences	1069:1079	arg1	ability					1097:1103	ability	1097:1103	ability	1097:1103	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	6	71	from	differences	1069:1079	arg1	activity					1084:1091	activity	1084:1091	activity	1084:1091	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	5	72	contain	possesses	943:951	arg2	CBM					996:998	CBM	996:998	CBM	996:998	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	5	72	contain	possesses	943:951	arg2	module					988:993	an additional carbohydrate-binding module	953:993	an additional carbohydrate-binding module (CBM)	953:999	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	5	72	contain	possesses	943:951	arg1	which					922:926	which	922:926	which	922:926	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	0	73	theme	fungus	101:106	arg1	emersonii					119:127	the fungus Rasamsonia emersonii	97:127	the fungus Rasamsonia emersonii	97:127	Structural and biochemical characterization of a family 7 highly thermostable endoglucanase from the fungus Rasamsonia emersonii.
31755197	9	74	theme	fewer	1573:1577	arg1	aromatics					1579:1587	fewer aromatics	1573:1587	fewer aromatics in the substrate-binding cleft	1573:1618	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	9	75	from	aromatics	1579:1587	arg1	cleft					1614:1618	the substrate-binding cleft	1592:1618	the substrate-binding cleft	1592:1618	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	12	76	from	available	1955:1963	arg1	database					1991:1998	RCSB Protein Data Bank database	1968:1998	RCSB Protein Data Bank database under the accession number 6SU8	1968:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	6	77	from	activity	1084:1091	arg1	number					1059:1064	a number	1057:1064	a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B	1057:1160	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	13	78	from	Rasamsonia emersonii	2082:2101	arg1	endoglucanase					2051:2063	endoglucanase (EC3.2.1.4)	2051:2075	endoglucanase (EC3.2.1.4) from Rasamsonia emersonii	2051:2101	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	2	79	theme	detailed	424:431	arg1	knowledge					444:452	detailed structural knowledge	424:452	detailed structural knowledge	424:452	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	3	80	theme	biochemical	473:483	arg1	data					500:503	substantial biochemical and structural data	461:503	substantial biochemical and structural data	461:503	While substantial biochemical and structural data are available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far.
31755197	5	81	theme	mesophilic	864:873	arg1	ascomycete					881:890	the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM)	860:999	ascomycete	881:890	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	12	82	theme	RCSB	1968:1971	arg1	database					1991:1998	RCSB Protein Data Bank database	1968:1998	RCSB Protein Data Bank database under the accession number 6SU8	1968:2030	DATABASE: Structural data are available in RCSB Protein Data Bank database under the accession number 6SU8.
31755197	9	83	theme	substrate-binding	1596:1612	arg1	cleft					1614:1618	the substrate-binding cleft	1592:1618	the substrate-binding cleft	1592:1618	Structural analyses revealed that ReCel7B has increased rigidity in a number of peripheral loops compared to TrCel7B and fewer aromatics in the substrate-binding cleft.
31755197	6	84	from	number	1059:1064	arg1	ability					1097:1103	ability	1097:1103	ability	1097:1103	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	6	84	from	number	1059:1064	arg1	activity					1084:1091	activity	1084:1091	activity	1084:1091	With a temperature optimum of 80 °C, ReCel7B displayed a number of differences in activity and ability to synergize with cellobiohydrolases compared to TrCel7B.
31755197	7	85	theme	ReCel7B	1226:1232	arg1	variant					1215:1221	a chimeric variant	1204:1221	a chimeric variant of ReCel7B and a CBM	1204:1242	We improved both binding and kinetics in a chimeric variant of ReCel7B and a CBM, while we observe the opposite effect when the CBM was removed in TrCel7B.
31755197	5	86	theme	model	875:879	arg1	ascomycete					881:890	the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM)	860:999	ascomycete	881:890	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	2	87	theme	structural	433:442	arg1	knowledge					444:452	detailed structural knowledge	424:452	detailed structural knowledge	424:452	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	5	88	theme	homologous	830:839	arg1	endoglucanase					841:853	the homologous endoglucanase	826:853	the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM)	826:999	The enzyme was compared with the homologous endoglucanase from the mesophilic model ascomycete Trichoderma reesei (TrCel7B), which unlike ReCel7B possesses an additional carbohydrate-binding module (CBM).
31755197	13	89	from	Trichoderma reesei	2212:2229	arg1	endoglucanase					2181:2193	endoglucanase (EC3.2.1.4)	2181:2205	endoglucanase (EC3.2.1.4) from Trichoderma reesei	2181:2229	ENZYMES: ReCel7B, endoglucanase (EC3.2.1.4) from Rasamsonia emersonii; ReCel7A, cellobiohydrolase (EC3.2.1.176) from Rasamsonia emersonii; TrCel7B, endoglucanase (EC3.2.1.4) from Trichoderma reesei; TrCel7A, cellobiohydrolase (EC3.2.1.176) from Trichoderma reesei.
31755197	8	90	theme	0.182	1421:1425	arg1	Rwork					1394:1398	Rwork	1394:1398	Rwork	1394:1398	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	8	90	theme	0.182	1421:1425	arg1	factors					1410:1416	Rfree factors	1404:1416	Rfree factors	1404:1416	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	3	91	theme	structural	489:498	arg1	data					500:503	substantial biochemical and structural data	461:503	substantial biochemical and structural data	461:503	While substantial biochemical and structural data are available for GH7 cellobiohydrolases, endoglucanases are more elusive and only few structures have been solved so far.
31755197	11	92	theme	structure-function	1757:1774	arg1	interpretations					1776:1790	Global structure-function interpretations	1750:1790	Global structure-function interpretations of ReCel7B	1750:1801	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	8	93	theme	0.206	1431:1435	arg1	Rwork					1394:1398	Rwork	1394:1398	Rwork	1394:1398	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	8	93	theme	0.206	1431:1435	arg1	factors					1410:1416	Rfree factors	1404:1416	Rfree factors	1404:1416	The crystal structure of ReCel7B was determined at 2.48 Å resolution, with Rwork and Rfree factors of 0.182 and 0.206, respectively.
31755197	2	94	theme	promising	354:362	arg1	strategy					364:371	a promising strategy	352:371	a promising strategy to alleviate the industrial costs	352:405	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	2	94	theme	promising	354:362	arg1	improvement					300:310	Activity improvement	291:310	Activity improvement of these enzymes via rational design	291:347	Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires detailed structural knowledge.
31755197	11	95	theme	GH7	1905:1907	arg1	endoglucanases					1909:1922	GH7 endoglucanases	1905:1922	GH7 endoglucanases	1905:1922	Global structure-function interpretations of ReCel7B highlight the differences in temperature stability, turnover, binding, and cellulose accessibility in GH7 endoglucanases.
31755197	1	96	theme	mixtures	231:238	arg1	cellulases					143:152	Thermostable cellulases	130:152	Thermostable cellulases from glycoside hydrolase family 7 (GH7)	130:192	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	96	theme	mixtures	231:238	arg1	components					207:216	the main components	198:216	the main components of enzymatic mixtures for industrial saccharification of lignocellulose	198:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31755197	1	97	theme	industrial	244:253	arg1	saccharification					255:270	industrial saccharification	244:270	industrial saccharification of lignocellulose	244:288	Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose.
31319211	0	0	theme	continuous	96:105	arg1	process					107:113	continuous process	96:113	continuous process	96:113	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	4	1	theme	SCB	676:678	arg1	100 g					667:671	100 g	667:671	100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively	667:783	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	1	2	theme	biomass	184:190	arg1	pretreatment					152:163	pretreatment	152:163	pretreatment of lignocellulosic biomass	152:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	5	3	theme	7.5 min	837:843	arg1	time					829:832	an average residence time	808:832	an average residence time of 7.5 min and 3.75 min	808:856	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	4	4	theme	released	567:574	arg1	sugars					576:581	The released sugars	563:581	The released sugars after enzymatic hydrolysis	563:608	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	3	5	dep	contents	553:560	arg1	regarding					518:526	regarding	518:526	regarding	518:526	Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents.
31319211	3	6	theme	cellulose	532:540	arg1	contents					553:560	the cellulose and lignin contents	528:560	the cellulose and lignin contents	528:560	Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents.
31319211	5	7	theme	glucose	882:888	arg1	32-38 g					871:877	32-38 g	871:877	32-38 g	871:877	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	5	7	theme	glucose	882:888	arg1	38-46 g					859:865	38-46 g	859:865	38-46 g	859:865	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	4	8	theme	enzymatic	589:597	arg1	hydrolysis					599:608	enzymatic hydrolysis	589:608	enzymatic hydrolysis	589:608	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	2	9	theme	alkaline	286:293	arg1	pretreatment					313:324	alkaline hydrogen peroxide pretreatment	286:324	alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB)	286:351	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	10	theme	sequential	356:365	arg1	SB-HC					376:380	SB-HC	376:380	SB-HC	376:380	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	10	theme	sequential	356:365	arg1	batches					367:373	sequential batches	356:373	sequential batches (SB-HC)	356:381	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	3	11	theme	compositional	466:478	arg1	modifications					480:492	compositional modifications	466:492	compositional modifications	466:492	Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents.
31319211	6	12	theme	evaluated	1066:1074	arg1	configurations					1076:1089	all evaluated configurations	1062:1089	all evaluated configurations aiming to produce high value bioproducts	1062:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	4	13	dep	B-HC	703:706	arg1	10 min					709:714	10 min	709:714	10 min of process	709:725	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	14	theme	SC-HC	732:736	arg1	process					738:744	SC-HC process	732:744	SC-HC process (7.5 min residence time)	732:769	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	14	theme	SC-HC	732:736	arg1	time					765:768	7.5 min residence time	747:768	7.5 min residence time	747:768	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	15	theme	glucose	655:661	arg1	32-35 g					644:650	32-35 g	644:650	32-35 g	644:650	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	15	theme	glucose	655:661	arg1	42 g					635:638	42 g	635:638	42 g	635:638	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	5	16	theme	SCB	903:905	arg1	100 g					894:898	100 g	894:898	100 g of SCB	894:905	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	1	17	theme	current	197:203	arg1	challenge					214:222	a current research challenge	195:222	a current research challenge	195:222	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	1	17	theme	current	197:203	arg1	Development					116:126	Development	116:126	Development of new technologies for pretreatment of lignocellulosic biomass	116:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	2	18	theme	sugarcane	329:337	arg1	SCB					348:350	SCB	348:350	SCB	348:350	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	18	theme	sugarcane	329:337	arg1	bagasse					339:345	sugarcane bagasse	329:345	sugarcane bagasse (SCB)	329:351	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	19	used	used	271:274	arg2	HC					263:264	HC	263:264	HC	263:264	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	19	used	used	271:274	arg2	cavitation					251:260	hydrodynamic cavitation	238:260	hydrodynamic cavitation (HC)	238:265	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	1	20	theme	research	205:212	arg1	challenge					214:222	a current research challenge	195:222	a current research challenge	195:222	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	1	20	theme	research	205:212	arg1	Development					116:126	Development	116:126	Development of new technologies for pretreatment of lignocellulosic biomass	116:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	2	21	theme	C-HC	424:427	arg1	processes					430:438	continuous (C-HC) processes	412:438	continuous (C-HC) processes	412:438	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	3	22	from	modifications	480:492	arg1	material					501:508	the material	497:508	the material	497:508	Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents.
31319211	5	23	theme	average	811:817	arg1	time					829:832	an average residence time	808:832	an average residence time of 7.5 min and 3.75 min	808:856	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	3	24	theme	lignin	546:551	arg1	contents					553:560	the cellulose and lignin contents	528:560	the cellulose and lignin contents	528:560	Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents.
31319211	6	25	from	configurations	1076:1089	arg1	pretreatment					1019:1030	pretreatment	1019:1030	pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts	1019:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	2	26	theme	continuous	412:421	arg1	processes					430:438	continuous (C-HC) processes	412:438	continuous (C-HC) processes	412:438	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	6	27	theme	value	1114:1118	arg1	bioproducts					1120:1130	high value bioproducts	1109:1130	high value bioproducts	1109:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	2	28	theme	peroxide	304:311	arg1	pretreatment					313:324	alkaline hydrogen peroxide pretreatment	286:324	alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB)	286:351	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	6	29	theme	lignocellulosic	1035:1049	arg1	biomass					1051:1057	lignocellulosic biomass	1035:1057	lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts	1035:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	0	30	theme	hydrodynamic	40:51	arg1	technology					64:73	hydrodynamic cavitation technology	40:73	hydrodynamic cavitation technology	40:73	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	5	31	theme	3.75 min	849:856	arg1	time					829:832	an average residence time	808:832	an average residence time of 7.5 min and 3.75 min	808:856	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	5	32	theme	enzymatic	937:945	arg1	step					958:961	enzymatic hydrolysis step	937:961	enzymatic hydrolysis step	937:961	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	5	33	theme	C-HC	789:792	arg1	process					794:800	C-HC process	789:800	C-HC process	789:800	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	4	34	theme	process	719:725	arg1	10 min					709:714	10 min	709:714	10 min of process	709:725	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	5	35	theme	hydrolysis	947:956	arg1	step					958:961	enzymatic hydrolysis step	937:961	enzymatic hydrolysis step	937:961	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	6	36	theme	HC	964:965	arg1	technology					967:976	HC technology	964:976	HC technology	964:976	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	6	36	theme	HC	964:965	arg1	alternative					1003:1013	a promising alternative	991:1013	a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts	991:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	2	37	theme	hydrodynamic	238:249	arg1	cavitation					251:260	hydrodynamic cavitation	238:260	hydrodynamic cavitation (HC)	238:265	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	2	37	theme	hydrodynamic	238:249	arg1	HC					263:264	HC	263:264	HC	263:264	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	6	38	from	biomass	1051:1057	arg1	configurations					1076:1089	all evaluated configurations	1062:1089	all evaluated configurations aiming to produce high value bioproducts	1062:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	1	39	theme	new	131:133	arg1	technologies					135:146	new technologies	131:146	new technologies for pretreatment of lignocellulosic biomass	131:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	0	40	theme	cavitation	53:62	arg1	technology					64:73	hydrodynamic cavitation technology	40:73	hydrodynamic cavitation technology	40:73	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	1	41	theme	technologies	135:146	arg1	challenge					214:222	a current research challenge	195:222	a current research challenge	195:222	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	1	41	theme	technologies	135:146	arg1	Development					116:126	Development	116:126	Development of new technologies for pretreatment of lignocellulosic biomass	116:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
31319211	4	42	theme	7.5 min	747:753	arg1	process					738:744	SC-HC process	732:744	SC-HC process (7.5 min residence time)	732:769	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	42	theme	7.5 min	747:753	arg1	time					765:768	7.5 min residence time	747:768	7.5 min residence time	747:768	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	0	43	dep	bagasse	26:32	arg1	Semi-continuous					76:90	Semi-continuous	76:90	Semi-continuous	76:90	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	0	43	dep	bagasse	26:32	arg1	process					107:113	continuous process	96:113	continuous process	96:113	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	6	44	theme	biomass	1051:1057	arg1	pretreatment					1019:1030	pretreatment	1019:1030	pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts	1019:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	6	45	theme	high	1109:1112	arg1	bioproducts					1120:1130	high value bioproducts	1109:1130	high value bioproducts	1109:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	2	46	theme	hydrogen	295:302	arg1	pretreatment					313:324	alkaline hydrogen peroxide pretreatment	286:324	alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB)	286:351	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	6	47	from	pretreatment	1019:1030	arg1	configurations					1076:1089	all evaluated configurations	1062:1089	all evaluated configurations aiming to produce high value bioproducts	1062:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	2	48	theme	bagasse	339:345	arg1	pretreatment					313:324	alkaline hydrogen peroxide pretreatment	286:324	alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB)	286:351	In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes.
31319211	4	49	theme	residence	755:763	arg1	process					738:744	SC-HC process	732:744	SC-HC process (7.5 min residence time)	732:769	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	4	49	theme	residence	755:763	arg1	time					765:768	7.5 min residence time	747:768	7.5 min residence time	747:768	The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively.
31319211	5	50	theme	residence	819:827	arg1	time					829:832	an average residence time	808:832	an average residence time of 7.5 min and 3.75 min	808:856	In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step.
31319211	6	51	theme	promising	993:1001	arg1	technology					967:976	HC technology	964:976	HC technology	964:976	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	6	51	theme	promising	993:1001	arg1	alternative					1003:1013	a promising alternative	991:1013	a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts	991:1130	HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.
31319211	0	52	theme	sugarcane	16:24	arg1	Pretreatment					0:11	Pretreatment	0:11	Pretreatment of sugarcane	0:24	Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.
31319211	1	53	theme	lignocellulosic	168:182	arg1	biomass					184:190	lignocellulosic biomass	168:190	lignocellulosic biomass	168:190	Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge.
30730130	0	0	theme	Wound	114:118	arg1	Healing					120:126	Burn-Related Wound Healing	101:126	Burn-Related Wound Healing	101:126	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	4	1	dep	encapsulated	597:608	arg1	whereas					698:704	whereas	698:704	whereas	698:704	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	5	2	theme	YSPs	936:939	arg1	effects					955:961	photothermal effects	942:961	photothermal effects	942:961	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	biocompatibility					861:876	biocompatibility	861:876	biocompatibility	861:876	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	structure					828:836	structure	828:836	structure	828:836	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	antioxidant					879:889	antioxidant	879:889	antioxidant	879:889	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	composition					848:858	chemical composition	839:858	chemical composition	839:858	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	profile					980:986	the release profile	968:986	the release profile of the encapsulated GLP	968:1010	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	effects					910:916	antibacterial effects	896:916	antibacterial effects	896:916	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	2	theme	YSPs	936:939	arg1	morphology					813:822	morphology	813:822	morphology	813:822	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	0	3	theme	Burn-Related	101:112	arg1	Healing					120:126	Burn-Related Wound Healing	101:126	Burn-Related Wound Healing	101:126	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	2	4	theme	great	368:372	arg1	challenge					374:382	a great challenge	366:382	a great challenge	366:382	However, the fabrication of polymer-based porous YSPs remains a great challenge.
30730130	6	5	theme	laser-assisted	1102:1115	arg1	therapy					1117:1123	the laser-assisted therapy	1098:1123	the laser-assisted therapy	1098:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	4	6	theme	lucidum	561:567	arg1	polysaccharides					569:583	Ganoderma lucidum polysaccharides	551:583	Ganoderma lucidum polysaccharides	551:583	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	7	theme	photothermal	789:800	arg1	nanoparticles					725:737	iron oxide (Fe3O4) nanoparticles	706:737	iron oxide (Fe3O4) nanoparticles	706:737	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	7	theme	photothermal	789:800	arg1	agent					802:806	a photothermal agent	787:806	a photothermal agent	787:806	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	8	theme	iron	706:709	arg1	Fe3O4					718:722	Fe3O4	718:722	Fe3O4	718:722	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	8	theme	iron	706:709	arg1	oxide					711:715	iron oxide	706:715	iron oxide (Fe3O4) nanoparticles	706:737	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	1	9	theme	excellent	276:284	arg1	capacity					294:301	excellent loading capacity	276:301	excellent loading capacity	276:301	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	5	10	theme	encapsulated	995:1006	arg1	GLP					1008:1010	the encapsulated GLP	991:1010	the encapsulated GLP	991:1010	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	4	11	theme	inner	766:770	arg1	core					772:775	the inner core	762:775	the inner core	762:775	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	1	12	theme	loading	286:292	arg1	capacity					294:301	excellent loading capacity	276:301	excellent loading capacity	276:301	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	6	13	theme	healing	1066:1072	arg1	effects					1074:1080	the in vivo wound healing effects	1048:1080	the in vivo wound healing effects of the YSPs and the laser-assisted therapy	1048:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	5	14	theme	GLP	1008:1010	arg1	effects					955:961	photothermal effects	942:961	photothermal effects	942:961	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	biocompatibility					861:876	biocompatibility	861:876	biocompatibility	861:876	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	structure					828:836	structure	828:836	structure	828:836	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	antioxidant					879:889	antioxidant	879:889	antioxidant	879:889	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	composition					848:858	chemical composition	839:858	chemical composition	839:858	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	profile					980:986	the release profile	968:986	the release profile of the encapsulated GLP	968:1010	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	effects					910:916	antibacterial effects	896:916	antibacterial effects	896:916	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	14	theme	GLP	1008:1010	arg1	morphology					813:822	morphology	813:822	morphology	813:822	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	1	15	theme	various	198:204	arg1	fields					215:220	various research fields	198:220	various research fields	198:220	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	6	16	dep	in	1052:1053	arg1	vivo					1055:1058	vivo	1055:1058	vivo	1055:1058	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	5	17	theme	chemical	839:846	arg1	composition					848:858	chemical composition	839:858	chemical composition	839:858	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	1	18	theme	research	206:213	arg1	fields					215:220	various research fields	198:220	various research fields	198:220	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	4	19	theme	TiO2-Ag	525:531	arg1	nanoparticles					533:545	TiO2-Ag nanoparticles	525:545	TiO2-Ag nanoparticles	525:545	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	19	theme	TiO2-Ag	525:531	arg1	GLPs					586:589	GLPs	586:589	GLPs	586:589	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	2	20	theme	polymer-based	332:344	arg1	YSPs					353:356	polymer-based porous YSPs	332:356	polymer-based porous YSPs	332:356	However, the fabrication of polymer-based porous YSPs remains a great challenge.
30730130	3	21	theme	multifunctional	399:413	arg1	YSPs					432:435	multifunctional polycaprolactone YSPs	399:435	multifunctional polycaprolactone YSPs	399:435	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	5	22	theme	release	972:978	arg1	profile					980:986	the release profile	968:986	the release profile of the encapsulated GLP	968:1010	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	3	23	theme	trineedle	457:465	arg1	electrospraying					475:489	trineedle coaxial electrospraying	457:489	trineedle coaxial electrospraying	457:489	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	6	24	theme	YSPs	1089:1092	arg1	effects					1074:1080	the in vivo wound healing effects	1048:1080	the in vivo wound healing effects of the YSPs and the laser-assisted therapy	1048:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	1	25	theme	Yolk-shell	129:138	arg1	YSPs					151:154	YSPs	151:154	YSPs	151:154	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	1	25	theme	Yolk-shell	129:138	arg1	particles					140:148	Yolk-shell particles	129:148	Yolk-shell particles (YSPs)	129:155	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	3	26	theme	simple	498:503	arg1	process					516:522	a simple nonsolvent process	496:522	a simple nonsolvent process	496:522	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	0	27	theme	Particle	18:25	arg1	Engineering					27:37	Particle Engineering	18:37	Particle Engineering	18:37	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	4	28	theme	outer	619:623	arg1	shell					625:629	the outer shell	615:629	the outer shell of the YSPs as the major antibacterial and antioxidant components	615:695	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	6	29	theme	burn	1150:1153	arg1	model					1161:1165	a burn wound model	1148:1165	a burn wound model on c57 mice	1148:1177	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	6	30	from	model	1161:1165	arg1	mice					1174:1177	c57 mice	1170:1177	c57 mice	1170:1177	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	3	31	theme	nonsolvent	505:514	arg1	process					516:522	a simple nonsolvent process	496:522	a simple nonsolvent process	496:522	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	0	32	theme	Nonsolvent-Assisted	43:61	arg1	Electrospraying					81:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying	43:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing	43:126	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	6	33	theme	wound	1060:1064	arg1	healing					1066:1072	the in vivo wound healing	1048:1072	the in vivo wound healing effects of the YSPs and the laser-assisted therapy	1048:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	4	34	theme	oxide	711:715	arg1	nanoparticles					725:737	iron oxide (Fe3O4) nanoparticles	706:737	iron oxide (Fe3O4) nanoparticles	706:737	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	4	34	theme	oxide	711:715	arg1	agent					802:806	a photothermal agent	787:806	a photothermal agent	787:806	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	1	35	theme	low	239:241	arg1	density					243:249	their low density	233:249	their low density	233:249	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	3	36	theme	coaxial	467:473	arg1	electrospraying					475:489	trineedle coaxial electrospraying	457:489	trineedle coaxial electrospraying	457:489	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	4	37	theme	antibacterial	656:668	arg1	components					686:695	the major antibacterial and antioxidant components	646:695	the major antibacterial and antioxidant components	646:695	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	3	38	theme	polycaprolactone	415:430	arg1	YSPs					432:435	multifunctional polycaprolactone YSPs	399:435	multifunctional polycaprolactone YSPs	399:435	In this work, multifunctional polycaprolactone YSPs were produced using trineedle coaxial electrospraying with a simple nonsolvent process.
30730130	5	39	theme	photothermal	942:953	arg1	effects					955:961	photothermal effects	942:961	photothermal effects	942:961	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	0	40	theme	Trineedle	63:71	arg1	Electrospraying					81:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying	43:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing	43:126	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	4	41	theme	YSPs	638:641	arg1	shell					625:629	the outer shell	615:629	the outer shell of the YSPs as the major antibacterial and antioxidant components	615:695	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	2	42	theme	YSPs	353:356	arg1	fabrication					317:327	the fabrication	313:327	the fabrication of polymer-based porous YSPs	313:356	However, the fabrication of polymer-based porous YSPs remains a great challenge.
30730130	6	43	theme	wound	1155:1159	arg1	model					1161:1165	a burn wound model	1148:1165	a burn wound model on c57 mice	1148:1177	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	6	44	theme	in	1052:1053	arg1	healing					1066:1072	the in vivo wound healing	1048:1072	the in vivo wound healing effects of the YSPs and the laser-assisted therapy	1048:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	6	45	theme	c57	1170:1172	arg1	mice					1174:1177	c57 mice	1170:1177	c57 mice	1170:1177	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	1	46	from	fields	215:220	arg1	attention					183:191	increasing attention	172:191	increasing attention from various research fields	172:220	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	2	47	theme	porous	346:351	arg1	YSPs					353:356	polymer-based porous YSPs	332:356	polymer-based porous YSPs	332:356	However, the fabrication of polymer-based porous YSPs remains a great challenge.
30730130	1	48	theme	large	252:256	arg1	area					266:269	large surface area	252:269	large surface area	252:269	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	6	49	theme	therapy	1117:1123	arg1	effects					1074:1080	the in vivo wound healing effects	1048:1080	the in vivo wound healing effects of the YSPs and the laser-assisted therapy	1048:1123	Furthermore, the in vivo wound healing effects of the YSPs and the laser-assisted therapy were explored based on a burn wound model on c57 mice.
30730130	4	50	theme	antioxidant	674:684	arg1	components					686:695	the major antibacterial and antioxidant components	646:695	the major antibacterial and antioxidant components	646:695	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	5	51	theme	fabricated	925:934	arg1	YSPs					936:939	the fabricated YSPs	921:939	the fabricated YSPs	921:939	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	5	52	theme	antibacterial	896:908	arg1	effects					910:916	antibacterial effects	896:916	antibacterial effects	896:916	The morphology and structure, chemical composition, biocompatibility, antioxidant, and antibacterial effects of the fabricated YSPs, photothermal effects, and the release profile of the encapsulated GLP were studied in vitro.
30730130	1	53	theme	surface	258:264	arg1	area					266:269	large surface area	252:269	large surface area	252:269	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	0	54	theme	Coaxial	73:79	arg1	Electrospraying					81:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying	43:95	Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing	43:126	Porous Yolk-Shell Particle Engineering via Nonsolvent-Assisted Trineedle Coaxial Electrospraying for Burn-Related Wound Healing.
30730130	4	55	theme	Ganoderma	551:559	arg1	lucidum					561:567	Ganoderma lucidum	551:567	Ganoderma lucidum polysaccharides	551:583	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30730130	1	56	theme	increasing	172:181	arg1	attention					183:191	increasing attention	172:191	increasing attention from various research fields	172:220	Yolk-shell particles (YSPs) have attracted increasing attention from various research fields because of their low density, large surface area, and excellent loading capacity.
30730130	4	57	theme	major	650:654	arg1	components					686:695	the major antibacterial and antioxidant components	646:695	the major antibacterial and antioxidant components	646:695	TiO2-Ag nanoparticles and Ganoderma lucidum polysaccharides (GLPs) were encapsulated into the outer shell of the YSPs as the major antibacterial and antioxidant components, whereas iron oxide (Fe3O4) nanoparticles were incorporated into the inner core to act as a photothermal agent.
30548527	7	0	theme	MCF-7	1191:1195	arg1	cells					1197:1201	MCF-7 cells	1191:1201	MCF-7 cells	1191:1201	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	1	1	theme	cancer	355:360	arg1	therapeutics					362:373	cancer therapeutics	355:373	cancer therapeutics	355:373	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	6	2	from	value	1006:1010	arg1	investigations					974:987	The drug release investigations	957:987	The drug release investigations in vitro at a pH value of 6.0 (acid environment)	957:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	6	3	theme	MTX	1065:1067	arg1	release					1054:1060	the release	1050:1060	the release of MTX	1050:1067	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	3	4	from	amount	703:708	arg1	mV					687:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV	660:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%	660:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	8	5	theme	promising	1373:1381	arg1	system					1392:1397	the promising delivery system	1369:1397	the promising delivery system for cancer therapeutics	1369:1421	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	6	6	theme	6.0	1015:1017	arg1	value					1006:1010	a pH value	1001:1010	a pH value of 6.0 (acid environment)	1001:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	8	7	theme	release	1340:1346	arg1	property					1348:1355	sustained drug release property	1325:1355	sustained drug release property	1325:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	5	8	dep	±	914:914	arg1	162.9					923:927	162.9	923:927	162.9	923:927	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	4	9	theme	obtained	743:750	arg1	various					798:804	various	798:804	various	798:804	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	9	theme	obtained	743:750	arg1	sizes					770:774	the obtained eventual particle sizes	739:774	the obtained eventual particle sizes of nanoparticles	739:791	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	8	10	theme	drug	1335:1338	arg1	property					1348:1355	sustained drug release property	1325:1355	sustained drug release property	1325:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	4	11	with	various	798:804	arg1	ratios					840:845	ratios	840:845	ratios	840:845	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	11	with	various	798:804	arg1	concentrations					821:834	different concentrations	811:834	different concentrations	811:834	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	10	12	theme	Part	1474:1477	arg1	A					1479:1479	Part A	1474:1479	J Biomed Mater Res Part A: 107A: 339-347, 2019.	1455:1501	J Biomed Mater Res Part A: 107A: 339-347, 2019.
30548527	3	13	from	loading	574:580	arg1	nanoparticles					589:601	the nanoparticles	585:601	the nanoparticles	585:601	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	5	14	dep	162.9	923:927	arg1	to					920:921	to	920:921	to	920:921	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	8	15	theme	sustained	1325:1333	arg1	property					1348:1355	sustained drug release property	1325:1355	sustained drug release property	1325:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	6	16	theme	in	989:990	arg1	investigations					974:987	The drug release investigations	957:987	The drug release investigations in vitro at a pH value of 6.0 (acid environment)	957:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	3	17	theme	%	723:723	arg1	%					723:723	13.3 ± 1.2%	713:723	13.3 ± 1.2%	713:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	3	17	theme	%	723:723	arg1	amount					703:708	a loading amount	693:708	a loading amount of 13.3 ± 1.2%	693:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	4	18	theme	nanoparticles	779:791	arg1	various					798:804	various	798:804	various	798:804	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	18	theme	nanoparticles	779:791	arg1	sizes					770:774	the obtained eventual particle sizes	739:774	the obtained eventual particle sizes of nanoparticles	739:791	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	6	19	dep	in	989:990	arg1	vitro					992:996	vitro	992:996	vitro	992:996	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	2	20	theme	disodium	474:481	arg1	composites					495:504	the methotrexate (MTX) disodium salt-loaded composites	451:504	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	7	21	from	efficacy	1151:1158	arg1	cells					1197:1201	MCF-7 cells	1191:1201	MCF-7 cells	1191:1201	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	7	21	from	efficacy	1151:1158	arg1	cells					1212:1216	HeLa cells	1207:1216	HeLa cells	1207:1216	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	7	22	theme	satisfactory	1226:1237	arg1	results					1239:1245	the satisfactory results	1222:1245	the satisfactory results	1222:1245	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	3	23	theme	zeta	620:623	arg1	values					635:640	zeta potential values	620:640	zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%	620:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	7	24	theme	anticancer	1140:1149	arg1	efficacy					1151:1158	the anticancer efficacy	1136:1158	the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells	1136:1216	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	2	25	theme	complex	418:424	arg1	nanoparticles					426:438	the designed polyelectrolyte complex nanoparticles	389:438	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	0	26	theme	Poly-allylamine	0:14	arg1	hydrochloride					16:28	Poly-allylamine hydrochloride	0:28	Poly-allylamine hydrochloride	0:28	Poly-allylamine hydrochloride and fucoidan-based self-assembled polyelectrolyte complex nanoparticles for cancer therapeutics.
30548527	3	27	theme	potential	625:633	arg1	values					635:640	zeta potential values	620:640	zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%	620:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	2	28	theme	polyelectrolyte	402:416	arg1	nanoparticles					426:438	the designed polyelectrolyte complex nanoparticles	389:438	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	0	29	theme	fucoidan-based	34:47	arg1	complex					80:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	Poly-allylamine hydrochloride and fucoidan-based self-assembled polyelectrolyte complex nanoparticles for cancer therapeutics.
30548527	6	30	theme	release	966:972	arg1	investigations					974:987	The drug release investigations	957:987	The drug release investigations in vitro at a pH value of 6.0 (acid environment)	957:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	4	31	theme	particle	761:768	arg1	various					798:804	various	798:804	various	798:804	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	31	theme	particle	761:768	arg1	sizes					770:774	the obtained eventual particle sizes	739:774	the obtained eventual particle sizes of nanoparticles	739:791	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	2	32	theme	methotrexate	455:466	arg1	composites					495:504	the methotrexate (MTX) disodium salt-loaded composites	451:504	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	5	33	theme	loading	944:950	arg1	MTX					952:954	loading MTX	944:954	loading MTX	944:954	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	6	34	theme	drug	961:964	arg1	investigations					974:987	The drug release investigations	957:987	The drug release investigations in vitro at a pH value of 6.0 (acid environment)	957:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	2	35	theme	designed	393:400	arg1	nanoparticles					426:438	the designed polyelectrolyte complex nanoparticles	389:438	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	4	36	theme	eventual	752:759	arg1	various					798:804	various	798:804	various	798:804	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	36	theme	eventual	752:759	arg1	sizes					770:774	the obtained eventual particle sizes	739:774	the obtained eventual particle sizes of nanoparticles	739:791	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	1	37	theme	poly-allylamine	232:246	arg1	PAH					263:265	PAH	263:265	PAH	263:265	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	1	37	theme	poly-allylamine	232:246	arg1	polyelectrolytes					214:229	two polyelectrolytes	210:229	two polyelectrolytes	210:229	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	1	37	theme	poly-allylamine	232:246	arg1	hydrochloride					248:260	poly-allylamine hydrochloride	232:260	poly-allylamine hydrochloride (PAH)	232:266	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	0	38	theme	polyelectrolyte	64:78	arg1	complex					80:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	Poly-allylamine hydrochloride and fucoidan-based self-assembled polyelectrolyte complex nanoparticles for cancer therapeutics.
30548527	5	39	theme	±	929:929	arg1	nm					935:936	130 ± 2.6 to 162.9 ± 2.3 nm	910:936	130 ± 2.6 to 162.9 ± 2.3 nm	910:936	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	6	40	theme	acid	1020:1023	arg1	environment					1025:1035	acid environment	1020:1035	acid environment	1020:1035	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	6	40	theme	acid	1020:1023	arg1	6.0					1015:1017	6.0	1015:1017	6.0	1015:1017	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
30548527	1	41	theme	polyelectrolytes	214:229	arg1	self-assembly					193:205	the self-assembly	189:205	the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively	189:326	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	5	42	theme	particle	872:879	arg1	size					881:884	The particle size	868:884	The particle size	868:884	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	0	43	theme	self-assembled	49:62	arg1	complex					80:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	fucoidan-based self-assembled polyelectrolyte complex	34:86	Poly-allylamine hydrochloride and fucoidan-based self-assembled polyelectrolyte complex nanoparticles for cancer therapeutics.
30548527	7	44	theme	MTX-loaded	1163:1172	arg1	nanoparticles					1174:1186	MTX-loaded nanoparticles	1163:1186	MTX-loaded nanoparticles	1163:1186	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	7	45	theme	nanoparticles	1174:1186	arg1	efficacy					1151:1158	the anticancer efficacy	1136:1158	the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells	1136:1216	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	3	46	theme	±	681:681	arg1	mV					687:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV	660:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%	660:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	4	47	theme	polyelectrolytes	850:865	arg1	ratios					840:845	ratios	840:845	ratios	840:845	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	4	47	theme	polyelectrolytes	850:865	arg1	concentrations					821:834	different concentrations	811:834	different concentrations	811:834	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	8	48	theme	PAH/fucoidan	1293:1304	arg1	nanoparticles					1306:1318	these self-assembled PAH/fucoidan nanoparticles	1272:1318	these self-assembled PAH/fucoidan nanoparticles with sustained drug release property	1272:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	2	49	theme	salt-loaded	483:493	arg1	composites					495:504	the methotrexate (MTX) disodium salt-loaded composites	451:504	the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites	389:504	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	1	50	theme	mild	335:338	arg1	conditions					340:349	mild conditions	335:349	mild conditions for cancer therapeutics	335:373	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	10	51	dep	Res	1470:1472	arg1	107A					1482:1485	107A	1482:1485	107A	1482:1485	J Biomed Mater Res Part A: 107A: 339-347, 2019.
30548527	10	51	dep	Res	1470:1472	arg1	A					1479:1479	Part A	1474:1479	J Biomed Mater Res Part A: 107A: 339-347, 2019.	1455:1501	J Biomed Mater Res Part A: 107A: 339-347, 2019.
30548527	3	52	theme	loading	695:701	arg1	%					723:723	13.3 ± 1.2%	713:723	13.3 ± 1.2%	713:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	3	52	theme	loading	695:701	arg1	amount					703:708	a loading amount	693:708	a loading amount of 13.3 ± 1.2%	693:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	8	53	theme	cancer	1403:1408	arg1	therapeutics					1410:1421	cancer therapeutics	1403:1421	cancer therapeutics	1403:1421	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	1	54	theme	novel	153:157	arg1	system					173:178	the novel drug delivery system	149:178	the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively	149:326	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	4	55	theme	different	811:819	arg1	concentrations					821:834	different concentrations	811:834	different concentrations	811:834	Furthermore, the obtained eventual particle sizes of nanoparticles were various with different concentrations and ratios of polyelectrolytes.
30548527	1	56	dep	polycation	289:298	arg1	the					285:287	the	285:287	the	285:287	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	1	57	theme	drug	159:162	arg1	system					173:178	the novel drug delivery system	149:178	the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively	149:326	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	8	58	theme	delivery	1383:1390	arg1	system					1392:1397	the promising delivery system	1369:1397	the promising delivery system for cancer therapeutics	1369:1421	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	3	59	dep	-28.3	675:679	arg1	to					672:673	to	672:673	to	672:673	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	8	60	with	nanoparticles	1306:1318	arg1	property					1348:1355	sustained drug release property	1325:1355	sustained drug release property	1325:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	1	61	theme	delivery	164:171	arg1	system					173:178	the novel drug delivery system	149:178	the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively	149:326	Herein, we fabricated the novel drug delivery system based on the self-assembly of two polyelectrolytes, poly-allylamine hydrochloride (PAH) and fucoidan, as the polycation and polyanion, respectively, under mild conditions for cancer therapeutics.
30548527	8	62	theme	self-assembled	1278:1291	arg1	nanoparticles					1306:1318	these self-assembled PAH/fucoidan nanoparticles	1272:1318	these self-assembled PAH/fucoidan nanoparticles with sustained drug release property	1272:1355	Together, these self-assembled PAH/fucoidan nanoparticles with sustained drug release property will become the promising delivery system for cancer therapeutics.
30548527	0	63	theme	cancer	106:111	arg1	therapeutics					113:124	cancer therapeutics	106:124	cancer therapeutics	106:124	Poly-allylamine hydrochloride and fucoidan-based self-assembled polyelectrolyte complex nanoparticles for cancer therapeutics.
30548527	9	64	dep	©	1424:1424	arg1	Inc.					1450:1453	Inc.	1450:1453	Inc.	1450:1453	© 2018 Wiley Periodicals, Inc.
30548527	10	65	dep	107A	1482:1485	arg1	339-347					1488:1494	339-347	1488:1494	339-347	1488:1494	J Biomed Mater Res Part A: 107A: 339-347, 2019.
30548527	3	66	theme	MTX	570:572	arg1	loading					574:580	The MTX loading	566:580	The MTX loading in the nanoparticles	566:601	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	7	67	theme	HeLa	1207:1210	arg1	cells					1212:1216	HeLa cells	1207:1216	HeLa cells	1207:1216	Finally, we investigated the anticancer efficacy of MTX-loaded nanoparticles on MCF-7 cells and HeLa cells and the satisfactory results were obtained.
30548527	2	68	theme	various	546:552	arg1	techniques					554:563	various techniques	546:563	various techniques	546:563	Furthermore, the designed polyelectrolyte complex nanoparticles as well as the methotrexate (MTX) disodium salt-loaded composites were systematically characterized using various techniques.
30548527	3	69	theme	±	718:718	arg1	%					723:723	13.3 ± 1.2%	713:723	13.3 ± 1.2%	713:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	3	70	theme	±	666:666	arg1	mV					687:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV	660:688	-36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%	660:723	The MTX loading in the nanoparticles was confirmed by zeta potential values that changed from -36.2 ± 2.2 to -28.3 ± 3.1 mV at a loading amount of 13.3 ± 1.2%.
30548527	5	71	theme	±	914:914	arg1	nm					935:936	130 ± 2.6 to 162.9 ± 2.3 nm	910:936	130 ± 2.6 to 162.9 ± 2.3 nm	910:936	The particle size also has increased from 130 ± 2.6 to 162.9 ± 2.3 nm after loading MTX.
30548527	6	72	theme	pH	1003:1004	arg1	value					1006:1010	a pH value	1001:1010	a pH value of 6.0 (acid environment)	1001:1036	The drug release investigations in vitro at a pH value of 6.0 (acid environment) showed that the release of MTX was sustained in the conditions provided.
31525970	0	0	theme	Melt	86:89	arg1	Stiffness					91:99	a High Melt Stiffness	79:99	a High Melt Stiffness	79:99	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	1	1	theme	elevated	322:329	arg1	temperatures					331:342	elevated temperatures	322:342	elevated temperatures	322:342	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	2	2	theme	resulting	349:357	arg1	plastics					369:376	The resulting thermoset plastics	345:376	The resulting thermoset plastics	345:376	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	2	3	theme	high	490:493	arg1	demand					495:500	high demand	490:500	high demand	490:500	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	7	4	theme	material	1344:1351	arg1	processability					1310:1323	the processability	1306:1323	the processability of a thermoplastic material	1306:1351	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	3	5	theme	thermoset-like	534:547	arg1	material					564:571	such a thermoset-like yet recyclable material	527:571	such a thermoset-like yet recyclable material	527:571	Here, we establish that such a thermoset-like yet recyclable material can be realized through the addition of a nanocellulose reinforcing agent.
31525970	9	6	theme	composite	1582:1590	arg1	printing					1558:1565	3D printing	1555:1565	3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin	1555:1660	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	8	7	theme	Repeated	1354:1361	arg1	re-extrusion					1363:1374	Repeated re-extrusion	1354:1374	Repeated re-extrusion at 140 °C	1354:1384	Repeated re-extrusion at 140 °C does not compromise the thermomechanical properties, which indicates a high degree of recyclability.
31525970	9	8	theme	resin	1656:1660	arg1	printability					1636:1647	the printability	1632:1647	the printability of the resin	1632:1660	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	5	9	theme	melt	916:919	arg1	stiffness					921:929	the melt stiffness	912:929	the melt stiffness of an otherwise molten ethylene-acrylate copolymer	912:980	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	5	10	theme	molten	947:952	arg1	copolymer					972:980	an otherwise molten ethylene-acrylate copolymer	934:980	an otherwise molten ethylene-acrylate copolymer	934:980	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	5	11	theme	wt	851:852	arg1	%					854:854	only 7.5 wt %	842:854	only 7.5 wt %	842:854	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	9	12	theme	3D	1555:1556	arg1	printing					1558:1565	3D printing	1555:1565	3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin	1555:1660	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	5	13	theme	material	880:887	arg1	addition					830:837	the addition	826:837	the addition of only 7.5 wt % of either nanocellulose material	826:887	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	0	14	theme	Creep	105:109	arg1	Resistance					111:120	Creep Resistance	105:120	Creep Resistance	105:120	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	2	15	with	materials	424:432	arg1	structure					473:481	a reconfigurable internal network structure	439:481	a reconfigurable internal network structure	439:481	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	2	16	theme	reconfigurable	441:454	arg1	structure					473:481	a reconfigurable internal network structure	439:481	a reconfigurable internal network structure	439:481	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	8	17	from	°C	1383:1384	arg1	re-extrusion					1363:1374	Repeated re-extrusion	1354:1374	Repeated re-extrusion at 140 °C	1354:1384	Repeated re-extrusion at 140 °C does not compromise the thermomechanical properties, which indicates a high degree of recyclability.
31525970	5	18	theme	nanocellulose	866:878	arg1	material					880:887	either nanocellulose material	859:887	either nanocellulose material	859:887	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	0	19	theme	Nanocellulose	8:20	arg1	Networks					22:29	Dynamic Nanocellulose Networks	0:29	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.	0:121	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	0	20	theme	High	81:84	arg1	Stiffness					91:99	a High Melt Stiffness	79:99	a High Melt Stiffness	79:99	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	7	21	theme	molten	1192:1197	arg1	composites					1199:1208	the molten composites	1188:1208	the molten composites	1188:1208	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	5	22	theme	ethylene-acrylate	954:970	arg1	copolymer					972:980	an otherwise molten ethylene-acrylate copolymer	934:980	an otherwise molten ethylene-acrylate copolymer	934:980	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	7	23	theme	high	1161:1164	arg1	rates					1172:1176	high shear rates	1161:1176	high shear rates	1161:1176	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	1	24	theme	low	183:185	arg1	point					195:199	a relatively low melting point	170:199	a relatively low melting point	170:199	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	0	25	theme	Dynamic	0:6	arg1	Networks					22:29	Dynamic Nanocellulose Networks	0:29	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.	0:121	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	4	26	theme	characteristics	738:752	arg1	many					726:729	many	726:729	many	726:729	A network consisting of cellulose nanocrystals, nano- or microfibrils imparts many of the characteristics that are usually achieved through chemical cross-linking.
31525970	4	26	theme	characteristics	738:752	arg1	characteristics					738:752	the characteristics	734:752	the characteristics that are usually achieved through chemical cross-linking	734:809	A network consisting of cellulose nanocrystals, nano- or microfibrils imparts many of the characteristics that are usually achieved through chemical cross-linking.
31525970	1	27	theme	melting	187:193	arg1	point					195:199	a relatively low melting point	170:199	a relatively low melting point	170:199	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	9	28	theme	high	1603:1606	arg1	stiffness					1613:1621	the high melt stiffness	1599:1621	the high melt stiffness	1599:1621	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	3	29	theme	recyclable	553:562	arg1	material					564:571	such a thermoset-like yet recyclable material	527:571	such a thermoset-like yet recyclable material	527:571	Here, we establish that such a thermoset-like yet recyclable material can be realized through the addition of a nanocellulose reinforcing agent.
31525970	9	30	theme	melt	1608:1611	arg1	stiffness					1613:1621	the high melt stiffness	1599:1621	the high melt stiffness	1599:1621	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	5	31	theme	copolymer	972:980	arg1	stiffness					921:929	the melt stiffness	912:929	the melt stiffness of an otherwise molten ethylene-acrylate copolymer	912:980	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	6	32	theme	molten	1129:1134	arg1	matrix					1136:1141	the neat molten matrix	1120:1141	the neat molten matrix	1120:1141	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
31525970	2	33	theme	alternative	412:422	arg1	materials					424:432	therefore alternative materials	402:432	therefore alternative materials with a reconfigurable internal network structure	402:481	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	1	34	from	temperatures	331:342	arg1	stiffness					288:296	a high stiffness	281:296	a high stiffness	281:296	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	1	34	from	temperatures	331:342	arg1	resistance					308:317	creep resistance	302:317	creep resistance	302:317	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	1	35	theme	high	283:286	arg1	stiffness					288:296	a high stiffness	281:296	a high stiffness	281:296	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	7	36	theme	copolymer	1267:1275	arg1	matrix					1277:1282	the copolymer matrix	1263:1282	the copolymer matrix	1263:1282	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	4	37	theme	cellulose	672:680	arg1	nanocrystals					682:693	cellulose nanocrystals	672:693	cellulose nanocrystals	672:693	A network consisting of cellulose nanocrystals, nano- or microfibrils imparts many of the characteristics that are usually achieved through chemical cross-linking.
31525970	5	38	theme	%	854:854	arg1	addition					830:837	the addition	826:837	the addition of only 7.5 wt % of either nanocellulose material	826:887	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	0	39	theme	Thermoset-like	35:48	arg1	Plastics					65:72	Thermoset-like yet Recyclable Plastics	35:72	Thermoset-like yet Recyclable Plastics	35:72	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	3	40	theme	agent	641:645	arg1	addition					601:608	the addition	597:608	the addition of a nanocellulose reinforcing agent	597:645	Here, we establish that such a thermoset-like yet recyclable material can be realized through the addition of a nanocellulose reinforcing agent.
31525970	6	41	theme	creep	1077:1081	arg1	elongation					1083:1092	the melt creep elongation	1068:1092	the melt creep elongation	1068:1092	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
31525970	9	42	theme	networks	1528:1535	arg1	versatility					1491:1501	The versatility	1487:1501	The versatility of dynamic nanocellulose networks	1487:1535	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	0	43	with	Networks	22:29	arg1	Stiffness					91:99	a High Melt Stiffness	79:99	a High Melt Stiffness	79:99	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	0	43	with	Networks	22:29	arg1	Resistance					111:120	Creep Resistance	105:120	Creep Resistance	105:120	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	6	44	theme	neat	1124:1127	arg1	matrix					1136:1141	the neat molten matrix	1120:1141	the neat molten matrix	1120:1141	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
31525970	0	45	theme	Recyclable	54:63	arg1	Plastics					65:72	Thermoset-like yet Recyclable Plastics	35:72	Thermoset-like yet Recyclable Plastics	35:72	Dynamic Nanocellulose Networks for Thermoset-like yet Recyclable Plastics with a High Melt Stiffness and Creep Resistance.
31525970	1	46	theme	creep	302:306	arg1	resistance					308:317	creep resistance	302:317	creep resistance	302:317	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	6	47	theme	melt	1072:1075	arg1	elongation					1083:1092	the melt creep elongation	1068:1092	the melt creep elongation	1068:1092	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
31525970	2	48	theme	network	465:471	arg1	structure					473:481	a reconfigurable internal network structure	439:481	a reconfigurable internal network structure	439:481	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	8	49	theme	high	1457:1460	arg1	degree					1462:1467	a high degree	1455:1467	a high degree of recyclability	1455:1484	Repeated re-extrusion at 140 °C does not compromise the thermomechanical properties, which indicates a high degree of recyclability.
31525970	1	50	theme	Many	123:126	arg1	polymers					128:135	Many polymers	123:135	Many polymers	123:135	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	1	50	theme	Many	123:126	arg1	polyethylene					148:159	polyethylene	148:159	polyethylene	148:159	Many polymers, including polyethylene, feature a relatively low melting point and hence must be cross-linked to make them viable for applications that demand a high stiffness and creep resistance at elevated temperatures.
31525970	5	51	theme	magnitude	1005:1013	arg1	order					996:1000	at least 1 order	985:1000	at least 1 order of magnitude	985:1013	For instance, the addition of only 7.5 wt % of either nanocellulose material significantly enhances the melt stiffness of an otherwise molten ethylene-acrylate copolymer by at least 1 order of magnitude.
31525970	3	52	theme	nanocellulose	615:627	arg1	agent					641:645	a nanocellulose reinforcing agent	613:645	a nanocellulose reinforcing agent	613:645	Here, we establish that such a thermoset-like yet recyclable material can be realized through the addition of a nanocellulose reinforcing agent.
31525970	9	53	theme	nanocellulose	1514:1526	arg1	networks					1528:1535	dynamic nanocellulose networks	1506:1535	dynamic nanocellulose networks	1506:1535	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	7	54	theme	higher	1241:1246	arg1	viscosity					1248:1256	a significantly higher viscosity	1225:1256	a significantly higher viscosity than the copolymer matrix	1225:1282	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	9	55	theme	cellulose	1572:1580	arg1	composite					1582:1590	a cellulose composite	1570:1590	a cellulose composite	1570:1590	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	4	56	theme	chemical	788:795	arg1	cross-linking					797:809	chemical cross-linking	788:809	chemical cross-linking	788:809	A network consisting of cellulose nanocrystals, nano- or microfibrils imparts many of the characteristics that are usually achieved through chemical cross-linking.
31525970	8	57	theme	recyclability	1472:1484	arg1	degree					1462:1467	a high degree	1455:1467	a high degree of recyclability	1455:1484	Repeated re-extrusion at 140 °C does not compromise the thermomechanical properties, which indicates a high degree of recyclability.
31525970	3	58	theme	reinforcing	629:639	arg1	agent					641:645	a nanocellulose reinforcing agent	613:645	a nanocellulose reinforcing agent	613:645	Here, we establish that such a thermoset-like yet recyclable material can be realized through the addition of a nanocellulose reinforcing agent.
31525970	2	59	theme	thermoset	359:367	arg1	plastics					369:376	The resulting thermoset plastics	345:376	The resulting thermoset plastics	345:376	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	6	60	theme	nanocellulose	1038:1050	arg1	network					1052:1058	the nanocellulose network	1034:1058	the nanocellulose network	1034:1058	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
31525970	9	61	theme	dynamic	1506:1512	arg1	networks					1528:1535	dynamic nanocellulose networks	1506:1535	dynamic nanocellulose networks	1506:1535	The versatility of dynamic nanocellulose networks is illustrated by 3D printing of a cellulose composite, where the high melt stiffness improves the printability of the resin.
31525970	7	62	theme	thermoplastic	1330:1342	arg1	material					1344:1351	a thermoplastic material	1328:1351	a thermoplastic material	1328:1351	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	8	63	theme	thermomechanical	1410:1425	arg1	properties					1427:1436	the thermomechanical properties	1406:1436	the thermomechanical properties	1406:1436	Repeated re-extrusion at 140 °C does not compromise the thermomechanical properties, which indicates a high degree of recyclability.
31525970	2	64	theme	internal	456:463	arg1	structure					473:481	a reconfigurable internal network structure	439:481	a reconfigurable internal network structure	439:481	The resulting thermoset plastics cannot be recycled, and therefore alternative materials with a reconfigurable internal network structure are in high demand.
31525970	7	65	theme	shear	1166:1170	arg1	rates					1172:1176	high shear rates	1161:1176	high shear rates	1161:1176	At high shear rates, however, the molten composites do not display a significantly higher viscosity than the copolymer matrix, and therefore retain the processability of a thermoplastic material.
31525970	6	66	theme	same	1023:1026	arg1	time					1028:1031	the same time	1019:1031	the same time	1019:1031	At the same time, the nanocellulose network reduces the melt creep elongation to less than 10%, whereas the neat molten matrix would rupture.
29853118	0	0	theme	breast	98:103	arg1	carcinomas					105:114	lung and breast carcinomas	89:114	carcinomas	105:114	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	4	1	theme	cancer	465:470	arg1	capabilities					486:497	the cancer cells killing capabilities	461:497	the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL)	461:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	7	2	theme	breast	1081:1086	arg1	cells					1106:1110	lung (A549) and breast carcinoma (MCF-7) cells	1065:1110	cells	1106:1110	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	6	3	theme	ELISA	711:715	arg1	detection					717:725	ELISA detection	711:725	ELISA detection	711:725	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	9	4	theme	cancer	1453:1458	arg1	agent					1472:1476	a promising cancer therapeutic agent	1441:1476	a promising cancer therapeutic agent	1441:1476	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	4	theme	cancer	1453:1458	arg1	it					1425:1426	it	1425:1426	it	1425:1426	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	7	5	theme	pretreated	1004:1013	arg1	PBL					1021:1023	nanocomposite pretreated human PBL	990:1023	nanocomposite pretreated human PBL	990:1023	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	5	6	dep	200 μg/ml	699:707	arg1	to					696:697	to	696:697	to	696:697	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	6	7	theme	CD56	884:887	arg1	expression					856:865	enhanced expression	847:865	enhanced expression of CD3, CD8, and CD56	847:887	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	7	8	theme	carcinoma	1088:1096	arg1	cells					1106:1110	lung (A549) and breast carcinoma (MCF-7) cells	1065:1110	cells	1106:1110	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	6	9	theme	cytokines	800:808	arg1	release					767:773	the release	763:773	the release of IFN-γ, IL-2 and TNF-α cytokines	763:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	9	10	theme	therapeutic	1460:1470	arg1	agent					1472:1476	a promising cancer therapeutic agent	1441:1476	a promising cancer therapeutic agent	1441:1476	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	10	theme	therapeutic	1460:1470	arg1	it					1425:1426	it	1425:1426	it	1425:1426	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	4	11	theme	killing	478:484	arg1	capabilities					486:497	the cancer cells killing capabilities	461:497	the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL)	461:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	6	12	theme	TNF-α	794:798	arg1	cytokines					800:808	TNF-α cytokines	794:808	TNF-α cytokines	794:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	9	13	theme	immunostimulatory	1352:1368	arg1	activities					1370:1379	the immunostimulatory activities	1348:1379	the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite	1348:1416	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	8	14	theme	cancer	1283:1288	arg1	death					1295:1299	the cancer cell death	1279:1299	the cancer cell death	1279:1299	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	5	15	theme	immunostimulatory	590:606	arg1	property					608:615	the immunostimulatory property	586:615	the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml)	586:708	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	6	16	theme	GG	947:948	arg1	treatment					950:958	GG treatment	947:958	GG treatment	947:958	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	6	17	theme	CD8	875:877	arg1	expression					856:865	enhanced expression	847:865	enhanced expression of CD3, CD8, and CD56	847:887	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	6	18	theme	IL-2	785:788	arg1	release					767:773	the release	763:773	the release of IFN-γ, IL-2 and TNF-α cytokines	763:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	8	19	theme	cell	1290:1293	arg1	death					1295:1299	the cancer cell death	1279:1299	the cancer cell death	1279:1299	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	8	20	theme	effector	1210:1217	arg1	ratios					1227:1232	effector: target ratios	1210:1232	effector: target ratios from 2.5:1 to 20:1	1210:1251	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	1	21	theme	precipitation	187:199	arg1	technique					201:209	precipitation technique	187:209	precipitation technique	187:209	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique.
29853118	4	22	used	used	404:407	arg2	PEC-GG-ZnO					389:398	PEC-GG-ZnO	389:398	PEC-GG-ZnO	389:398	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	4	22	used	used	404:407	arg2	immunomodulator					415:429	an immunomodulator	412:429	an immunomodulator for the first time	412:448	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	6	23	theme	IFN-γ	778:782	arg1	release					767:773	the release	763:773	the release of IFN-γ, IL-2 and TNF-α cytokines	763:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	8	24	from	increase	1267:1274	arg1	death					1295:1299	the cancer cell death	1279:1299	the cancer cell death	1279:1299	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	0	25	theme	Pectin-guar	0:10	arg1	nanocomposite					27:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	9	26	theme	PEC-GG-ZnO	1384:1393	arg1	activities					1370:1379	the immunostimulatory activities	1348:1379	the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite	1348:1416	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	0	27	theme	gum-zinc	12:19	arg1	nanocomposite					27:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	6	28	theme	CD3	870:872	arg1	expression					856:865	enhanced expression	847:865	enhanced expression of CD3, CD8, and CD56	847:887	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	5	29	theme	PEC-GG-ZnO	624:633	arg1	property					608:615	the immunostimulatory property	586:615	the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml)	586:708	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	0	30	theme	lung	89:92	arg1	carcinomas					105:114	lung and breast carcinomas	89:114	carcinomas	105:114	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	6	31	from	increase	751:758	arg1	release					767:773	the release	763:773	the release of IFN-γ, IL-2 and TNF-α cytokines	763:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	3	32	theme	50-70 nm	370:377	arg1	range					361:365	the size range	352:365	the size range of 50-70 nm	352:377	TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm.
29853118	7	33	theme	lung	1065:1068	arg1	A549					1071:1074	lung (A549) and breast carcinoma (MCF-7) cells	1065:1110	A549	1071:1074	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	5	34	theme	proliferation	559:571	arg1	assay					573:577	The lymphocyte proliferation assay	544:577	The lymphocyte proliferation assay	544:577	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	3	35	theme	size	356:359	arg1	range					361:365	the size range	352:365	the size range of 50-70 nm	352:377	TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm.
29853118	7	36	theme	enhanced	1035:1042	arg1	cytotoxicity					1044:1055	enhanced cytotoxicity	1035:1055	enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells	1035:1110	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	8	37	theme	microcytotoxicity	1146:1162	arg1	assay					1164:1168	The microcytotoxicity assay	1142:1168	The microcytotoxicity assay	1142:1168	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	1	38	theme	Pectin-guar	117:127	arg1	nanocomposite					157:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite	117:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite	117:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique.
29853118	8	39	theme	target	1220:1225	arg1	ratios					1227:1232	effector: target ratios	1210:1232	effector: target ratios from 2.5:1 to 20:1	1210:1251	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	0	40	theme	oxide	21:25	arg1	nanocomposite					27:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite	0:39	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	1	41	theme	gum-zinc	129:136	arg1	PEC-GG-ZnO					145:154	PEC-GG-ZnO	145:154	PEC-GG-ZnO	145:154	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique.
29853118	1	41	theme	gum-zinc	129:136	arg1	oxide					138:142	gum-zinc oxide	129:142	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite	117:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique.
29853118	0	42	theme	human	50:54	arg1	lymphocytes					56:66	human lymphocytes	50:66	human lymphocytes cytotoxicity towards lung and breast carcinomas	50:114	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	7	43	theme	untreated	1127:1135	arg1	PBL					1137:1139	untreated PBL	1127:1139	untreated PBL	1127:1139	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	4	44	theme	human	502:506	arg1	PBL					538:540	PBL	538:540	PBL	538:540	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	4	44	theme	human	502:506	arg1	lymphocytes					525:535	human peripheral-blood lymphocytes	502:535	human peripheral-blood lymphocytes (PBL)	502:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	4	45	theme	first	439:443	arg1	time					445:448	the first time	435:448	the first time	435:448	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	7	46	theme	nanocomposite	990:1002	arg1	PBL					1021:1023	nanocomposite pretreated human PBL	990:1023	nanocomposite pretreated human PBL	990:1023	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	3	47	theme	nanocomposite	333:345	arg1	shape					324:328	the hexagonal shape	310:328	the hexagonal shape of nanocomposite	310:345	TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm.
29853118	8	48	from	2.5:1	1239:1243	arg1	ratios					1227:1232	effector: target ratios	1210:1232	effector: target ratios from 2.5:1 to 20:1	1210:1251	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	8	48	from	2.5:1	1239:1243	arg1	increase					1198:1205	increase	1198:1205	increase in effector: target ratios from 2.5:1 to 20:1	1198:1251	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	5	49	from	increase	660:667	arg1	200 μg/ml					699:707	25 μg/ml to 200 μg/ml	687:707	25 μg/ml to 200 μg/ml	687:707	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	5	49	from	increase	660:667	arg1	concentration					672:684	concentration	672:684	concentration (25 μg/ml to 200 μg/ml)	672:708	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	3	50	theme	TEM	292:294	arg1	images					296:301	TEM images	292:301	TEM images	292:301	TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm.
29853118	6	51	dep	PEC-GG-ZnO	913:922	arg1	compared					927:934	compared	927:934	compared to PEC and GG treatment	927:958	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	1	52	theme	oxide	138:142	arg1	nanocomposite					157:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite	117:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite	117:169	Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique.
29853118	0	53	theme	lymphocytes	56:66	arg1	cytotoxicity					68:79	human lymphocytes cytotoxicity	50:79	human lymphocytes cytotoxicity towards lung and breast carcinomas	50:114	Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
29853118	4	54	theme	lymphocytes	525:535	arg1	capabilities					486:497	the cancer cells killing capabilities	461:497	the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL)	461:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	7	55	theme	human	1015:1019	arg1	PBL					1021:1023	nanocomposite pretreated human PBL	990:1023	nanocomposite pretreated human PBL	990:1023	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	8	56	from	increase	1198:1205	arg1	2.5:1					1239:1243	2.5:1	1239:1243	2.5:1	1239:1243	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	8	56	from	increase	1198:1205	arg1	ratios					1227:1232	effector: target ratios	1210:1232	effector: target ratios from 2.5:1 to 20:1	1210:1251	The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death.
29853118	6	57	theme	enhanced	847:854	arg1	expression					856:865	enhanced expression	847:865	enhanced expression of CD3, CD8, and CD56	847:887	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	4	58	theme	peripheral-blood	508:523	arg1	PBL					538:540	PBL	538:540	PBL	538:540	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	4	58	theme	peripheral-blood	508:523	arg1	lymphocytes					525:535	human peripheral-blood lymphocytes	502:535	human peripheral-blood lymphocytes (PBL)	502:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	6	59	theme	cytometry	819:827	arg1	analysis					829:836	flow cytometry analysis	814:836	flow cytometry analysis	814:836	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	7	60	theme	MCF-7	1099:1103	arg1	cells					1106:1110	lung (A549) and breast carcinoma (MCF-7) cells	1065:1110	cells	1106:1110	Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL.
29853118	6	61	theme	significant	739:749	arg1	increase					751:758	a significant increase	737:758	a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines	737:808	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	5	62	theme	lymphocyte	548:557	arg1	assay					573:577	The lymphocyte proliferation assay	544:577	The lymphocyte proliferation assay	544:577	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	5	63	theme	25 μg/ml	687:694	arg1	200 μg/ml					699:707	25 μg/ml to 200 μg/ml	687:707	25 μg/ml to 200 μg/ml	687:707	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	5	63	theme	25 μg/ml	687:694	arg1	concentration					672:684	concentration	672:684	concentration (25 μg/ml to 200 μg/ml)	672:708	The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml).
29853118	6	64	theme	flow	814:817	arg1	analysis					829:836	flow cytometry analysis	814:836	flow cytometry analysis	814:836	ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment.
29853118	3	65	theme	hexagonal	314:322	arg1	shape					324:328	the hexagonal shape	310:328	the hexagonal shape of nanocomposite	310:345	TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm.
29853118	9	66	theme	novel	1398:1402	arg1	nanocomposite					1404:1416	a novel nanocomposite	1396:1416	a novel nanocomposite	1396:1416	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	66	theme	novel	1398:1402	arg1	PEC-GG-ZnO					1384:1393	PEC-GG-ZnO	1384:1393	PEC-GG-ZnO	1384:1393	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	67	theme	current	1322:1328	arg1	data					1330:1333	the current data	1318:1333	the current data	1318:1333	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	68	dep	corroborates	1335:1346	arg1	serve					1432:1436	serve	1432:1436	can serve as a promising cancer therapeutic agent	1428:1476	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	4	69	theme	cells	472:476	arg1	capabilities					486:497	the cancer cells killing capabilities	461:497	the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL)	461:541	Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL).
29853118	9	70	theme	promising	1443:1451	arg1	agent					1472:1476	a promising cancer therapeutic agent	1441:1476	a promising cancer therapeutic agent	1441:1476	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
29853118	9	70	theme	promising	1443:1451	arg1	it					1425:1426	it	1425:1426	it	1425:1426	Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.
30342285	12	0	theme	injectable	1799:1808	arg1	bone					1810:1813	the injectable bone	1795:1813	the injectable bone	1795:1813	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	16	1	theme	osteoinductive	2731:2744	arg1	environment					2746:2756	an osteoinductive environment	2728:2756	an osteoinductive environment for osteoblast differentiation and osteogenesis	2728:2804	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	11	2	theme	control	1682:1688	arg1	species					1690:1696	the control species	1678:1696	the control species	1678:1696	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	3	3	theme	bone	569:572	arg1	regeneration					574:585	bone regeneration	569:585	bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity	569:660	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	16	4	dep	substitutes	2587:2597	arg1	provided					2599:2606	provided	2599:2606	substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis	2587:2804	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	8	5	theme	CG	1196:1197	arg1	%					1191:1191	1.5 and 2.5 wt%	1177:1191	1.5 and 2.5 wt%	1177:1191	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	8	5	theme	CG	1196:1197	arg1	CG					1196:1197	CG	1196:1197	CG	1196:1197	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	6	6	theme	Cell	930:933	arg1	studies					943:949	Cell culture studies	930:949	Cell culture studies	930:949	Cell culture studies indicated that all samples tested were cytocompatible.
30342285	15	7	from	mineralization	2468:2481	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	11	8	theme	phosphatase	1593:1603	arg1	analyses					1634:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	3	9	theme	materials	555:563	arg1	materials					555:563	the most investigated materials	533:563	the most investigated materials	533:563	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	3	9	theme	materials	555:563	arg1	one					526:528	one	526:528	one	526:528	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	14	10	theme	complex	2214:2220	arg1	defects					2222:2228	geometric complex defects	2204:2228	geometric complex defects	2204:2228	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	13	11	theme	SIGNIFICANCE	1965:1976	arg1	STATEMENT					1952:1960	STATEMENT	1952:1960	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.	1952:2162	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	1	12	theme	bone	180:183	arg1	material					196:203	a bone substitute material	178:203	a bone substitute material	178:203	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material has been underexplored to date.
30342285	8	13	theme	S1	1282:1283	arg1	materials					1290:1298	the S1 test materials	1278:1298	the S1 test materials which were comprised of 1 wt% of CG	1278:1334	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	16	14	theme	cellular	2658:2665	arg1	growth					2667:2672	cellular growth	2658:2672	cellular growth	2658:2672	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	16	15	dep	carrageenan	2556:2566	arg1	the					2552:2554	the	2552:2554	the	2552:2554	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	8	16	theme	cell	1242:1245	arg1	capacity					1257:1264	cell spreading capacity	1242:1264	cell spreading capacity	1242:1264	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	9	17	theme	Excellent	1337:1345	arg1	viability					1358:1366	Excellent osteoblast viability	1337:1366	Excellent osteoblast viability	1337:1366	Excellent osteoblast viability and adhesion were observed for each of the tested materials.
30342285	4	18	theme	composites	747:756	arg1	preparation					718:728	the preparation	714:728	the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications	714:812	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	14	19	theme	injectable	2169:2178	arg1	biomaterial					2180:2190	This injectable biomaterial	2164:2190	This injectable biomaterial	2164:2190	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	7	20	theme	scaffold	1099:1106	arg1	surfaces					1108:1115	all the scaffold surfaces	1091:1115	all the scaffold surfaces that were tested	1091:1132	Relative to control substrates, cellular attachment and proliferation were better on all the scaffold surfaces that were tested.
30342285	13	21	theme	materials	2117:2125	arg1	behavior					2064:2071	the in vitro cytocompatibility behavior	2033:2071	the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2033:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	15	22	theme	injectable	2501:2510	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	4	23	theme	κ-CG/nHA	761:768	arg1	composites					747:756	new bioactive composites	733:756	new bioactive composites of κ-CG/nHA	733:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	4	23	theme	κ-CG/nHA	761:768	arg1	κ-CG/nHA					761:768	κ-CG/nHA	761:768	κ-CG/nHA	761:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	11	24	theme	test	1759:1762	arg1	surfaces					1773:1780	test κ-CG/nHA surfaces	1759:1780	test κ-CG/nHA surfaces	1759:1780	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	7	25	theme	cellular	1038:1045	arg1	attachment					1047:1056	cellular attachment	1038:1056	cellular attachment	1038:1056	Relative to control substrates, cellular attachment and proliferation were better on all the scaffold surfaces that were tested.
30342285	13	26	theme	carrageenan	2076:2086	arg1	materials					2117:2125	carrageenan and hydroxyapatite composite materials	2076:2125	carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2076:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	4	27	theme	new	733:735	arg1	composites					747:756	new bioactive composites	733:756	new bioactive composites of κ-CG/nHA	733:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	4	27	theme	new	733:735	arg1	κ-CG/nHA					761:768	κ-CG/nHA	761:768	κ-CG/nHA	761:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	13	28	theme	hydroxyapatite	2092:2105	arg1	materials					2117:2125	carrageenan and hydroxyapatite composite materials	2076:2125	carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2076:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	13	29	dep	STATEMENT	1952:1960	arg1	lies					2004:2007	lies	2004:2007	lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2004:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	14	30	theme	bone	2275:2278	arg1	capacity					2293:2300	high bone regeneration capacity	2270:2300	bioactivity as well as high bone regeneration capacity	2247:2300	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	16	31	theme	great	2629:2633	arg1	capacity					2635:2642	a great capacity	2627:2642	a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis	2627:2804	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	15	32	with	contact	2368:2374	arg1	proliferation					2428:2440	proliferation	2428:2440	proliferation	2428:2440	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	15	32	with	contact	2368:2374	arg1	metabolism					2452:2461	cellular metabolism	2443:2461	cellular metabolism	2443:2461	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	15	32	with	contact	2368:2374	arg1	adhesion					2415:2422	cellular adhesion	2406:2422	cellular adhesion	2406:2422	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	15	32	with	contact	2368:2374	arg1	mineralization					2468:2481	mineralization	2468:2481	mineralization on the fabricated injectable bone substitutes	2468:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	15	32	with	contact	2368:2374	arg1	scaffolds					2385:2393	the scaffolds	2381:2393	the scaffolds	2381:2393	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	13	33	theme	behavior	2064:2071	arg1	determination					2016:2028	the determination	2012:2028	the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2012:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	2	34	theme	cellular	450:457	arg1	adhesion					459:466	cellular adhesion	450:466	cellular adhesion	450:466	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	4	35	theme	numerous	774:781	arg1	applications					801:812	numerous bone regeneration applications	774:812	numerous bone regeneration applications	774:812	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	0	36	theme	Osteoblast	0:9	arg1	responses					11:19	Osteoblast responses	0:19	Osteoblast responses to injectable bone	0:38	Osteoblast responses to injectable bone substitutes of kappa-carrageenan and nano hydroxyapatite.
30342285	2	37	contain	have	256:259	arg2	characteristics					272:286	remarkable characteristics	261:286	remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs)	261:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	37	contain	have	256:259	arg2	biocompatibility					296:311	biocompatibility	296:311	biocompatibility	296:311	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	37	contain	have	256:259	arg2	similarities					345:356	structural similarities	334:356	structural similarities with natural glycosaminoglycans (GAGs)	334:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	37	contain	have	256:259	arg2	hydrophilicity					314:327	hydrophilicity	314:327	hydrophilicity	314:327	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	37	contain	have	256:259	arg1	Carrageenans					237:248	Carrageenans	237:248	Carrageenans (CGs)	237:254	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	37	contain	have	256:259	arg1	CGs					251:253	CGs	251:253	CGs	251:253	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	16	38	theme	osteoblast	2762:2771	arg1	differentiation					2773:2787	osteoblast differentiation	2762:2787	osteoblast differentiation	2762:2787	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	15	39	from	proliferation	2428:2440	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	10	40	theme	osteoconductive	1510:1524	arg1	environment					1526:1536	a distinct osteoconductive environment	1499:1536	a distinct osteoconductive environment	1499:1536	Additionally, the bone substitutes developed for this study presented a distinct osteoconductive environment.
30342285	4	41	theme	regeneration	788:799	arg1	applications					801:812	numerous bone regeneration applications	774:812	numerous bone regeneration applications	774:812	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	14	42	theme	regeneration	2280:2291	arg1	capacity					2293:2300	high bone regeneration capacity	2270:2300	bioactivity as well as high bone regeneration capacity	2247:2300	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	9	43	theme	osteoblast	1347:1356	arg1	viability					1358:1366	Excellent osteoblast viability	1337:1366	Excellent osteoblast viability	1337:1366	Excellent osteoblast viability and adhesion were observed for each of the tested materials.
30342285	12	44	theme	bone	1883:1886	arg1	applications					1901:1912	numerous bone regeneration applications	1874:1912	numerous bone regeneration applications	1874:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	1	45	theme	kappa-carrageenan	117:133	arg1	combination					102:112	The combination	98:112	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material	98:203	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material has been underexplored to date.
30342285	12	46	dep	substitute	1815:1824	arg1	developed					1826:1834	developed	1826:1834	substitute developed here demonstrated great potential for numerous bone regeneration applications	1815:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	8	47	theme	S2	1139:1140	arg1	samples					1149:1155	The S2 and S3 samples	1135:1155	The S2 and S3 samples	1135:1155	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	8	47	theme	S2	1139:1140	arg1	those					1158:1162	those	1158:1162	those	1158:1162	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	2	48	with	similarities	345:356	arg1	GAGs					391:394	GAGs	391:394	GAGs	391:394	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	48	with	similarities	345:356	arg1	glycosaminoglycans					371:388	natural glycosaminoglycans	363:388	natural glycosaminoglycans (GAGs)	363:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	11	49	theme	ALP	1699:1701	arg1	expression					1703:1712	ALP expression	1699:1712	ALP expression	1699:1712	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	5	50	dep	bone	891:894	arg1	substitutes					896:906	substitutes	896:906	substitutes	896:906	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	11	51	theme	calcium	1615:1621	arg1	deposition					1623:1632	calcium deposition	1615:1632	calcium deposition	1615:1632	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	5	52	theme	in	837:838	arg1	experiments					846:856	in vitro experiments	837:856	in vitro experiments	837:856	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	8	53	theme	S3	1146:1147	arg1	samples					1149:1155	The S2 and S3 samples	1135:1155	The S2 and S3 samples	1135:1155	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	8	53	theme	S3	1146:1147	arg1	those					1158:1162	those	1158:1162	those	1158:1162	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	14	54	theme	high	2270:2273	arg1	capacity					2293:2300	high bone regeneration capacity	2270:2300	bioactivity as well as high bone regeneration capacity	2247:2300	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	13	55	theme	in	2037:2038	arg1	behavior					2064:2071	the in vitro cytocompatibility behavior	2033:2071	the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2033:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	3	56	theme	Hydroxyapatite	487:500	arg1	nanoparticles					502:514	Hydroxyapatite nanoparticles	487:514	Hydroxyapatite nanoparticles	487:514	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	15	57	theme	osteoblast	2348:2357	arg1	cells					2359:2363	osteoblast cells	2348:2363	osteoblast cells	2348:2363	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	2	58	theme	natural	363:369	arg1	GAGs					391:394	GAGs	391:394	GAGs	391:394	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	58	theme	natural	363:369	arg1	glycosaminoglycans					371:388	natural glycosaminoglycans	363:388	natural glycosaminoglycans (GAGs)	363:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	9	59	theme	tested	1411:1416	arg1	materials					1418:1426	the tested materials	1407:1426	the tested materials	1407:1426	Excellent osteoblast viability and adhesion were observed for each of the tested materials.
30342285	16	60	with	biomaterial	2610:2620	arg1	capacity					2635:2642	a great capacity	2627:2642	a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis	2627:2804	The results show than the carrageenan and hydroxyapatite substitutes provided a biomaterial with a great capacity for promoting cellular growth, adhesion, and proliferation, as well as contributing an osteoinductive environment for osteoblast differentiation and osteogenesis.
30342285	6	61	theme	culture	935:941	arg1	studies					943:949	Cell culture studies	930:949	Cell culture studies	930:949	Cell culture studies indicated that all samples tested were cytocompatible.
30342285	8	62	theme	CG	1333:1334	arg1	%					1328:1328	1 wt%	1324:1328	1 wt% of CG	1324:1334	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	8	62	theme	CG	1333:1334	arg1	CG					1333:1334	CG	1333:1334	CG	1333:1334	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	2	63	theme	remarkable	261:270	arg1	characteristics					272:286	remarkable characteristics	261:286	remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs)	261:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	63	theme	remarkable	261:270	arg1	biocompatibility					296:311	biocompatibility	296:311	biocompatibility	296:311	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	63	theme	remarkable	261:270	arg1	similarities					345:356	structural similarities	334:356	structural similarities with natural glycosaminoglycans (GAGs)	334:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	63	theme	remarkable	261:270	arg1	hydrophilicity					314:327	hydrophilicity	314:327	hydrophilicity	314:327	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	5	64	theme	bone	891:894	arg1	influence					874:882	the influence	870:882	the influence of the bone substitutes on human osteoblasts	870:927	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	8	65	from	enhancement	1227:1237	arg1	capacity					1257:1264	cell spreading capacity	1242:1264	cell spreading capacity	1242:1264	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	5	66	dep	in	837:838	arg1	vitro					840:844	vitro	840:844	vitro	840:844	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	8	67	theme	spreading	1247:1255	arg1	capacity					1257:1264	cell spreading capacity	1242:1264	cell spreading capacity	1242:1264	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	12	68	theme	great	1854:1858	arg1	potential					1860:1868	great potential	1854:1868	great potential for numerous bone regeneration applications	1854:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	15	69	theme	cellular	2443:2450	arg1	metabolism					2452:2461	cellular metabolism	2443:2461	cellular metabolism	2443:2461	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	14	70	theme	geometric	2204:2212	arg1	defects					2222:2228	geometric complex defects	2204:2228	geometric complex defects	2204:2228	This injectable biomaterial can fill in geometric complex defects, and it displays bioactivity as well as high bone regeneration capacity.
30342285	11	71	theme	alkaline	1584:1591	arg1	ALP					1606:1608	ALP	1606:1608	ALP	1606:1608	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	11	71	theme	alkaline	1584:1591	arg1	phosphatase					1593:1603	alkaline phosphatase	1584:1603	alkaline phosphatase (ALP)	1584:1609	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	15	72	theme	cellular	2406:2413	arg1	adhesion					2415:2422	cellular adhesion	2406:2422	cellular adhesion	2406:2422	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	5	73	theme	human	911:915	arg1	osteoblasts					917:927	human osteoblasts	911:927	human osteoblasts	911:927	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	8	74	theme	test	1285:1288	arg1	materials					1290:1298	the S1 test materials	1278:1298	the S1 test materials which were comprised of 1 wt% of CG	1278:1334	The S2 and S3 samples, those permeated by 1.5 and 2.5 wt% of CG, respectively, exhibited an enhancement in cell spreading capacity compared to the S1 test materials which were comprised of 1 wt% of CG.
30342285	3	75	theme	excellent	600:608	arg1	biocompatibility					610:625	their excellent biocompatibility	594:625	their excellent biocompatibility	594:625	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	3	76	dep	investigated	542:553	arg1	most					537:540	most	537:540	most	537:540	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	4	77	theme	bioactive	737:745	arg1	composites					747:756	new bioactive composites	733:756	new bioactive composites of κ-CG/nHA	733:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	4	77	theme	bioactive	737:745	arg1	κ-CG/nHA					761:768	κ-CG/nHA	761:768	κ-CG/nHA	761:768	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	5	78	from	influence	874:882	arg1	osteoblasts					917:927	human osteoblasts	911:927	human osteoblasts	911:927	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	11	79	theme	κ-CG/nHA	1764:1771	arg1	surfaces					1773:1780	test κ-CG/nHA surfaces	1759:1780	test κ-CG/nHA surfaces	1759:1780	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	13	80	theme	injectable	2135:2144	arg1	bone					2146:2149	injectable bone substitutes	2135:2161	injectable bone substitutes	2135:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	15	81	theme	fabricated	2490:2499	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	1	82	theme	hydroxyapatite	146:159	arg1	combination					102:112	The combination	98:112	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material	98:203	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material has been underexplored to date.
30342285	2	83	with	biocompatibility	296:311	arg1	GAGs					391:394	GAGs	391:394	GAGs	391:394	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	83	with	biocompatibility	296:311	arg1	glycosaminoglycans					371:388	natural glycosaminoglycans	363:388	natural glycosaminoglycans (GAGs)	363:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	13	84	theme	composite	2107:2115	arg1	materials					2117:2125	carrageenan and hydroxyapatite composite materials	2076:2125	carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2076:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	7	85	theme	control	1018:1024	arg1	substrates					1026:1035	control substrates	1018:1035	control substrates	1018:1035	Relative to control substrates, cellular attachment and proliferation were better on all the scaffold surfaces that were tested.
30342285	2	86	with	hydrophilicity	314:327	arg1	GAGs					391:394	GAGs	391:394	GAGs	391:394	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	2	86	with	hydrophilicity	314:327	arg1	glycosaminoglycans					371:388	natural glycosaminoglycans	363:388	natural glycosaminoglycans (GAGs)	363:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	13	87	theme	cytocompatibility	2046:2062	arg1	behavior					2064:2071	the in vitro cytocompatibility behavior	2033:2071	the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes	2033:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	13	88	dep	in	2037:2038	arg1	vitro					2040:2044	vitro	2040:2044	vitro	2040:2044	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	3	89	theme	investigated	542:553	arg1	materials					555:563	the most investigated materials	533:563	the most investigated materials	533:563	Hydroxyapatite nanoparticles have been one of the most investigated materials for bone regeneration due to their excellent biocompatibility, bioactivity and osteoconductivity.
30342285	0	90	theme	injectable	24:33	arg1	bone					35:38	injectable bone	24:38	injectable bone	24:38	Osteoblast responses to injectable bone substitutes of kappa-carrageenan and nano hydroxyapatite.
30342285	5	91	theme	experiments	846:856	arg1	experiments					846:856	in vitro experiments	837:856	in vitro experiments	837:856	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	5	91	theme	experiments	846:856	arg1	set					830:832	a set	828:832	a set of in vitro experiments	828:856	We performed a set of in vitro experiments to evaluate the influence of the bone substitutes on human osteoblasts.
30342285	12	92	theme	regeneration	1888:1899	arg1	applications					1901:1912	numerous bone regeneration applications	1874:1912	numerous bone regeneration applications	1874:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	10	93	theme	distinct	1501:1508	arg1	environment					1526:1536	a distinct osteoconductive environment	1499:1536	a distinct osteoconductive environment	1499:1536	Additionally, the bone substitutes developed for this study presented a distinct osteoconductive environment.
30342285	4	94	attach	presents	689:696	arg1	particular					666:675	particular	666:675	particular	666:675	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	4	94	attach	presents	689:696	arg2	study					683:687	this study	678:687	this study	678:687	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	4	95	theme	bone	783:786	arg1	applications					801:812	numerous bone regeneration applications	774:812	numerous bone regeneration applications	774:812	In particular, this study presents an approach for the preparation of new bioactive composites of κ-CG/nHA for numerous bone regeneration applications.
30342285	12	96	dep	developed	1826:1834	arg1	demonstrated					1841:1852	demonstrated	1841:1852	substitute developed here demonstrated great potential for numerous bone regeneration applications	1815:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	15	97	dep	bone	2512:2515	arg1	substitutes					2517:2527	substitutes	2517:2527	substitutes	2517:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	0	98	dep	kappa-carrageenan	55:71	arg1	hydroxyapatite					82:95	hydroxyapatite	82:95	hydroxyapatite	82:95	Osteoblast responses to injectable bone substitutes of kappa-carrageenan and nano hydroxyapatite.
30342285	15	99	from	metabolism	2452:2461	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	11	100	attach	derived	1571:1577	arg1	analyses					1634:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	11	100	attach	derived	1571:1577	arg2	Data					1539:1542	Data	1539:1542	Data supporting this claim	1539:1564	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	10	101	dep	substitutes	1452:1462	arg1	developed					1464:1472	developed	1464:1472	substitutes developed for this study presented a distinct osteoconductive environment	1452:1536	Additionally, the bone substitutes developed for this study presented a distinct osteoconductive environment.
30342285	2	102	theme	structural	334:343	arg1	similarities					345:356	structural similarities	334:356	structural similarities with natural glycosaminoglycans (GAGs)	334:395	Carrageenans (CGs) have remarkable characteristics such as biocompatibility, hydrophilicity, and structural similarities with natural glycosaminoglycans (GAGs), and they have demonstrated the ability to stimulate cellular adhesion and proliferation.
30342285	12	103	theme	numerous	1874:1881	arg1	applications					1901:1912	numerous bone regeneration applications	1874:1912	numerous bone regeneration applications	1874:1912	In summary, the injectable bone substitute developed here demonstrated great potential for numerous bone regeneration applications, and thus, should be studied further.
30342285	11	104	theme	calcium	1718:1724	arg1	deposition					1726:1735	calcium deposition	1718:1735	calcium deposition	1718:1735	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	13	105	theme	work	1999:2002	arg1	novelty					1983:1989	The novelty	1979:1989	The novelty of this work	1979:2002	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	15	106	theme	cells	2359:2363	arg1	behaviors					2335:2343	the behaviors	2331:2343	the behaviors of osteoblast cells	2331:2363	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
30342285	13	107	dep	bone	2146:2149	arg1	substitutes					2151:2161	substitutes	2151:2161	substitutes	2151:2161	STATEMENT OF SIGNIFICANCE: The novelty of this work lies in the determination of the in vitro cytocompatibility behavior of carrageenan and hydroxyapatite composite materials used as injectable bone substitutes.
30342285	1	108	dep	material	196:203	arg1	substitute					185:194	substitute	185:194	substitute	185:194	The combination of kappa-carrageenan (κ-CG) and hydroxyapatite (HA) to generate a bone substitute material has been underexplored to date.
30342285	11	109	theme	deposition	1623:1632	arg1	analyses					1634:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	alkaline phosphatase (ALP) and calcium deposition analyses	1584:1641	Data supporting this claim were derived from alkaline phosphatase (ALP) and calcium deposition analyses, which indicated that, compared to the control species, ALP expression and calcium deposition were both improved on test κ-CG/nHA surfaces.
30342285	15	110	from	adhesion	2415:2422	arg1	bone					2512:2515	the fabricated injectable bone	2486:2515	the fabricated injectable bone substitutes	2486:2527	In this study, we evaluated the behaviors of osteoblast cells in contact with the scaffolds, including cellular adhesion and proliferation, cellular metabolism, and mineralization on the fabricated injectable bone substitutes.
29681215	2	0	theme	gel	584:586	arg1	contraction					588:598	gel contraction	584:598	gel contraction	584:598	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	3	1	theme	composite	668:676	arg1	gels					678:681	the composite gels	664:681	the composite gels	664:681	The microstructure and the mechanical strength of the composite gels were altered by varying HAD concentrations.
29681215	2	2	theme	different	355:363	arg1	concentrations					365:378	different concentrations	355:378	different concentrations of hyaluronic acid dialdehyde (HAD)	355:414	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	9	3	theme	in	1645:1646	arg1	culture					1654:1660	in vitro culture	1645:1660	in vitro culture	1645:1660	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	3	4	theme	HAD	707:709	arg1	concentrations					711:724	HAD concentrations	707:724	HAD concentrations	707:724	The microstructure and the mechanical strength of the composite gels were altered by varying HAD concentrations.
29681215	6	5	from	changes	1242:1248	arg1	cell					1253:1256	cell morphology and migration	1253:1281	cell	1253:1256	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	6	5	from	changes	1242:1248	arg1	migration					1273:1281	migration	1273:1281	migration	1273:1281	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	1	6	from	concentrations	297:310	arg1	gels					334:337	gels	334:337	gels	334:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	10	7	theme	HAD	1766:1768	arg1	gels					1770:1773	high HAD gels	1761:1773	high HAD gels	1761:1773	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	1	8	theme	much	203:206	arg1	importance					208:217	not much importance	199:217	not much importance	199:217	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	6	9	theme	mechanical	1127:1136	arg1	strength					1138:1145	hydrogel mechanical strength	1118:1145	hydrogel mechanical strength	1118:1145	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	10	10	theme	cellular	1704:1711	arg1	behavior					1713:1720	the cellular behavior	1700:1720	the cellular behavior in damaged cartilage	1700:1741	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	8	11	theme	matrix	1422:1427	arg1	contraction					1429:1439	matrix contraction	1422:1439	matrix contraction	1422:1439	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	11	12	theme	hydrogel	1891:1898	arg1	compositions					1900:1911	hydrogel compositions	1891:1911	hydrogel compositions	1891:1911	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	0	13	theme	Chondrocyte	25:35	arg1	Morphology					37:46	Chondrocyte Morphology	25:46	Chondrocyte Morphology	25:46	Hyaluronic Acid Dictates Chondrocyte Morphology and Migration in Composite Gels.
29681215	2	14	theme	cytoskeletal	553:564	arg1	organization					566:577	cytoskeletal organization	553:577	cytoskeletal organization	553:577	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	9	15	from	changes	1561:1567	arg1	responses					1577:1585	cell responses	1572:1585	cell responses	1572:1585	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	6	16	theme	protrusions	1201:1211	arg1	strength					1138:1145	hydrogel mechanical strength	1118:1145	hydrogel mechanical strength	1118:1145	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	6	16	theme	protrusions	1201:1211	arg1	organization					1161:1172	cytoskeletal organization	1148:1172	cytoskeletal organization	1148:1172	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	6	16	theme	protrusions	1201:1211	arg1	formation					1179:1187	formation	1179:1187	formation of cellular protrusions, all of which contributed to changes in cell morphology and migration	1179:1281	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	5	17	theme	cytoskeleton	932:943	arg1	organization					912:923	The organization	908:923	The organization of the cytoskeleton at the cellular protrusions	908:971	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	4	18	from	increase	795:802	arg1	number					842:847	number	842:847	number of cell protrusions	842:867	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	4	18	from	increase	795:802	arg1	ratio					820:824	their aspect ratio	807:824	their aspect ratio	807:824	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	8	19	theme	protein	1464:1470	arg1	vinculin					1472:1479	adhesion protein vinculin	1455:1479	adhesion protein vinculin	1455:1479	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	7	20	theme	cell-encapsulated	1322:1338	arg1	gels					1340:1343	3D cell-encapsulated gels	1319:1343	3D cell-encapsulated gels	1319:1343	These changes were more evident in 3D cell-encapsulated gels than chondrocytes cultured over the 2D gels.
29681215	0	21	theme	Composite	65:73	arg1	Gels					75:78	Composite Gels	65:78	Composite Gels	65:78	Hyaluronic Acid Dictates Chondrocyte Morphology and Migration in Composite Gels.
29681215	10	22	theme	healthy	1801:1807	arg1	tissue					1819:1824	healthy cartilage tissue	1801:1824	healthy cartilage tissue	1801:1824	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	1	23	theme	tissue	170:175	arg1	engineering					177:187	cartilage tissue engineering	160:187	cartilage tissue engineering	160:187	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	5	24	from	tip	1029:1031	arg1	vimentin					977:984	vimentin	977:984	vimentin localized at the base	977:1006	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	24	from	tip	1029:1031	arg1	microtubules					1009:1020	microtubules	1009:1020	microtubules at the tip	1009:1031	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	24	from	tip	1029:1031	arg1	actin					1038:1042	actin	1038:1042	actin	1038:1042	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	8	25	theme	hyaluronic	1486:1495	arg1	acid					1497:1500	hyaluronic acid	1486:1500	hyaluronic acid receptor CD44	1486:1514	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	5	26	theme	cell	1069:1072	arg1	body					1074:1077	the cell body	1065:1077	the cell body	1065:1077	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	27	theme	cellular	952:959	arg1	protrusions					961:971	the cellular protrusions	948:971	the cellular protrusions	948:971	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	11	28	used	used	1955:1958	arg2	models					1972:1977	in vitro models	1963:1977	in vitro models	1963:1977	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	11	28	used	used	1955:1958	arg2	matrices					1937:1944	biomimetic matrices	1926:1944	biomimetic matrices that are used as in vitro models to study chondrocyte behavior	1926:2007	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	0	29	from	Migration	52:60	arg1	Gels					75:78	Composite Gels	65:78	Composite Gels	65:78	Hyaluronic Acid Dictates Chondrocyte Morphology and Migration in Composite Gels.
29681215	11	30	theme	compositions	1900:1911	arg1	importance					1853:1862	the importance	1849:1862	the importance of careful formulations of hydrogel compositions	1849:1911	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	4	31	from	decrease	830:837	arg1	number					842:847	number	842:847	number of cell protrusions	842:867	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	4	31	from	decrease	830:837	arg1	ratio					820:824	their aspect ratio	807:824	their aspect ratio	807:824	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	10	32	from	behavior	1789:1796	arg1	tissue					1819:1824	healthy cartilage tissue	1801:1824	healthy cartilage tissue	1801:1824	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	1	33	theme	Tissue	81:86	arg1	hydrogels					130:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	7	34	theme	3D	1319:1320	arg1	gels					1340:1343	3D cell-encapsulated gels	1319:1343	3D cell-encapsulated gels	1319:1343	These changes were more evident in 3D cell-encapsulated gels than chondrocytes cultured over the 2D gels.
29681215	2	35	theme	collagen-HAD	457:468	arg1	gels					485:488	collagen-HAD composite (CH) gels	457:488	collagen-HAD composite (CH) gels	457:488	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	0	36	from	Morphology	37:46	arg1	Gels					75:78	Composite Gels	65:78	Composite Gels	65:78	Hyaluronic Acid Dictates Chondrocyte Morphology and Migration in Composite Gels.
29681215	8	37	from	compositions	1543:1554	arg1	similar					1525:1531	similar	1525:1531	similar	1525:1531	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	2	38	theme	dialdehyde	399:408	arg1	concentrations					365:378	different concentrations	355:378	different concentrations of hyaluronic acid dialdehyde (HAD)	355:414	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	0	39	theme	Hyaluronic	0:9	arg1	Acid					11:14	Hyaluronic Acid	0:14	Hyaluronic Acid	0:14	Hyaluronic Acid Dictates Chondrocyte Morphology and Migration in Composite Gels.
29681215	1	40	theme	varying	289:295	arg1	concentrations					297:310	varying concentrations	289:310	varying concentrations of hyaluronic acid in gels	289:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	1	41	theme	collagen-hyaluronic	99:117	arg1	hydrogels					130:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	4	42	theme	chondrocytes	741:752	arg1	Morphology					727:736	Morphology	727:736	Morphology of chondrocytes cultured on CH gels	727:772	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	10	43	theme	low	1681:1683	arg1	gels					1689:1692	low HAD gels	1681:1692	low HAD gels	1681:1692	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	8	44	theme	CH	1540:1541	arg1	compositions					1543:1554	all CH compositions	1536:1554	all CH compositions	1536:1554	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	1	45	theme	acid	326:329	arg1	concentrations					297:310	varying concentrations	289:310	varying concentrations of hyaluronic acid in gels	289:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	9	46	theme	matrix	1620:1625	arg1	deposition					1627:1636	extracellular matrix deposition	1606:1636	extracellular matrix deposition	1606:1636	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	10	47	from	responses	1668:1676	arg1	gels					1689:1692	low HAD gels	1681:1692	low HAD gels	1681:1692	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	11	48	theme	biomimetic	1926:1935	arg1	models					1972:1977	in vitro models	1963:1977	in vitro models	1963:1977	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	11	48	theme	biomimetic	1926:1935	arg1	matrices					1937:1944	biomimetic matrices	1926:1944	biomimetic matrices that are used as in vitro models to study chondrocyte behavior	1926:2007	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	2	49	theme	hyaluronic	383:392	arg1	dialdehyde					399:408	hyaluronic acid dialdehyde	383:408	hyaluronic acid dialdehyde (HAD)	383:414	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	5	50	dep	localized	1044:1052	arg1	vimentin					977:984	vimentin	977:984	vimentin localized at the base	977:1006	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	50	dep	localized	1044:1052	arg1	microtubules					1009:1020	microtubules	1009:1020	microtubules at the tip	1009:1031	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	50	dep	localized	1044:1052	arg1	actin					1038:1042	actin	1038:1042	actin	1038:1042	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	1	51	from	gels	334:337	arg1	concentrations					297:310	varying concentrations	289:310	varying concentrations of hyaluronic acid in gels	289:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	5	52	located	localized	986:994	arg2	vimentin					977:984	vimentin	977:984	vimentin localized at the base	977:1006	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	5	52	located	localized	986:994	arg1	base					1003:1006	the base	999:1006	the base	999:1006	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	3	53	theme	gels	678:681	arg1	strength					652:659	the mechanical strength	637:659	the mechanical strength of the composite gels	637:681	The microstructure and the mechanical strength of the composite gels were altered by varying HAD concentrations.
29681215	3	53	theme	gels	678:681	arg1	microstructure					618:631	The microstructure	614:631	The microstructure	614:631	The microstructure and the mechanical strength of the composite gels were altered by varying HAD concentrations.
29681215	1	54	from	acid	326:329	arg1	gels					334:337	gels	334:337	gels	334:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	6	55	dep	cell	1253:1256	arg1	morphology					1258:1267	morphology	1258:1267	morphology	1258:1267	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	4	56	theme	protrusions	857:867	arg1	number					842:847	number	842:847	number of cell protrusions	842:867	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	11	57	theme	chondrocyte	1988:1998	arg1	behavior					2000:2007	chondrocyte behavior	1988:2007	chondrocyte behavior	1988:2007	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	10	58	from	behavior	1713:1720	arg1	cartilage					1733:1741	damaged cartilage	1725:1741	damaged cartilage	1725:1741	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	6	59	theme	cytoskeletal	1148:1159	arg1	organization					1161:1172	cytoskeletal organization	1148:1172	cytoskeletal organization	1148:1172	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	1	60	used	used	151:154	arg2	hydrogels					130:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	4	61	theme	HAD	903:905	arg1	concentration					886:898	concentration	886:898	concentration of HAD	886:905	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	8	62	theme	contraction	1429:1439	arg1	CD44					1511:1514	hyaluronic acid receptor CD44	1486:1514	hyaluronic acid receptor CD44	1486:1514	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	8	62	theme	contraction	1429:1439	arg1	staining					1442:1449	staining	1442:1449	staining for adhesion protein vinculin	1442:1479	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	8	62	theme	contraction	1429:1439	arg1	viability					1399:1407	viability	1399:1407	viability of cells and matrix contraction	1399:1439	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	6	63	theme	hydrogel	1118:1125	arg1	strength					1138:1145	hydrogel mechanical strength	1118:1145	hydrogel mechanical strength	1118:1145	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	11	64	dep	in	1963:1964	arg1	vitro					1966:1970	vitro	1966:1970	vitro	1966:1970	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	2	65	theme	cell	519:522	arg1	shape					524:528	cell shape	519:528	cell shape	519:528	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	7	66	theme	2D	1381:1382	arg1	gels					1384:1387	the 2D gels	1377:1387	the 2D gels	1377:1387	These changes were more evident in 3D cell-encapsulated gels than chondrocytes cultured over the 2D gels.
29681215	8	67	theme	adhesion	1455:1462	arg1	vinculin					1472:1479	adhesion protein vinculin	1455:1479	adhesion protein vinculin	1455:1479	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	6	68	theme	cellular	1192:1199	arg1	protrusions					1201:1211	cellular protrusions	1192:1211	cellular protrusions	1192:1211	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	10	69	dep	HAD	1766:1768	arg1	high					1761:1764	high	1761:1764	high	1761:1764	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	4	70	from	increase	874:881	arg1	concentration					886:898	concentration	886:898	concentration of HAD	886:905	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	10	71	theme	cartilage	1809:1817	arg1	tissue					1819:1824	healthy cartilage tissue	1801:1824	healthy cartilage tissue	1801:1824	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	1	72	theme	cartilage	160:168	arg1	engineering					177:187	cartilage tissue engineering	160:187	cartilage tissue engineering	160:187	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	8	73	theme	acid	1497:1500	arg1	CD44					1511:1514	hyaluronic acid receptor CD44	1486:1514	hyaluronic acid receptor CD44	1486:1514	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	7	74	from	gels	1340:1343	arg1	evident					1308:1314	evident	1308:1314	evident	1308:1314	These changes were more evident in 3D cell-encapsulated gels than chondrocytes cultured over the 2D gels.
29681215	4	75	theme	cell	852:855	arg1	protrusions					857:867	cell protrusions	852:867	cell protrusions	852:867	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	4	76	theme	CH	766:767	arg1	gels					769:772	CH gels	766:772	CH gels	766:772	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	1	77	theme	equivalent	88:97	arg1	hydrogels					130:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	8	78	theme	receptor	1502:1509	arg1	CD44					1511:1514	hyaluronic acid receptor CD44	1486:1514	hyaluronic acid receptor CD44	1486:1514	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	11	79	theme	careful	1867:1873	arg1	formulations					1875:1886	careful formulations	1867:1886	careful formulations	1867:1886	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	2	80	theme	acid	394:397	arg1	dialdehyde					399:408	hyaluronic acid dialdehyde	383:408	hyaluronic acid dialdehyde (HAD)	383:414	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	9	81	dep	in	1645:1646	arg1	vitro					1648:1652	vitro	1648:1652	vitro	1648:1652	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	1	82	theme	acid-based	119:128	arg1	hydrogels					130:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels	81:138	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	10	83	dep	mimic	1694:1698	arg1	whereas					1744:1750	whereas	1744:1750	whereas	1744:1750	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	10	84	theme	damaged	1725:1731	arg1	cartilage					1733:1741	damaged cartilage	1725:1741	damaged cartilage	1725:1741	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	8	85	from	similar	1525:1531	arg1	compositions					1543:1554	all CH compositions	1536:1554	all CH compositions	1536:1554	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	1	86	theme	hyaluronic	315:324	arg1	acid					326:329	hyaluronic acid	315:329	hyaluronic acid in gels	315:337	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
29681215	9	87	theme	cell	1572:1575	arg1	responses					1577:1585	cell responses	1572:1585	cell responses	1572:1585	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	2	88	theme	CH	481:482	arg1	gels					485:488	collagen-HAD composite (CH) gels	457:488	collagen-HAD composite (CH) gels	457:488	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	10	89	theme	Cell	1663:1666	arg1	responses					1668:1676	Cell responses	1663:1676	Cell responses in low HAD gels	1663:1692	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	2	90	theme	composite	470:478	arg1	gels					485:488	collagen-HAD composite (CH) gels	457:488	collagen-HAD composite (CH) gels	457:488	In this study, different concentrations of hyaluronic acid dialdehyde (HAD) were combined with collagen to fabricate collagen-HAD composite (CH) gels, and the influence of HAD on cell shape, migration, viability, cytoskeletal organization, and gel contraction was examined.
29681215	4	91	theme	aspect	813:818	arg1	ratio					820:824	their aspect ratio	807:824	their aspect ratio	807:824	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	4	92	theme	significant	783:793	arg1	increase					795:802	a significant increase	781:802	a significant increase in their aspect ratio	781:824	Morphology of chondrocytes cultured on CH gels showed a significant increase in their aspect ratio and decrease in number of cell protrusions with increase in concentration of HAD.
29681215	9	93	theme	extracellular	1606:1618	arg1	deposition					1627:1636	extracellular matrix deposition	1606:1636	extracellular matrix deposition	1606:1636	The changes in cell responses further influenced extracellular matrix deposition during in vitro culture.
29681215	11	94	theme	in	1963:1964	arg1	models					1972:1977	in vitro models	1963:1977	in vitro models	1963:1977	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	11	94	theme	in	1963:1964	arg1	matrices					1937:1944	biomimetic matrices	1926:1944	biomimetic matrices that are used as in vitro models to study chondrocyte behavior	1926:2007	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	6	95	theme	HAD	1091:1093	arg1	concentrations					1095:1108	HAD concentrations	1091:1108	HAD concentrations	1091:1108	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	3	96	theme	mechanical	641:650	arg1	strength					652:659	the mechanical strength	637:659	the mechanical strength of the composite gels	637:681	The microstructure and the mechanical strength of the composite gels were altered by varying HAD concentrations.
29681215	10	97	theme	HAD	1685:1687	arg1	gels					1689:1692	low HAD gels	1681:1692	low HAD gels	1681:1692	Cell responses in low HAD gels mimic the cellular behavior in damaged cartilage, whereas those in high HAD gels resembled the behavior in healthy cartilage tissue.
29681215	7	98	from	evident	1308:1314	arg1	gels					1340:1343	3D cell-encapsulated gels	1319:1343	3D cell-encapsulated gels	1319:1343	These changes were more evident in 3D cell-encapsulated gels than chondrocytes cultured over the 2D gels.
29681215	8	99	theme	cells	1412:1416	arg1	CD44					1511:1514	hyaluronic acid receptor CD44	1486:1514	hyaluronic acid receptor CD44	1486:1514	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	8	99	theme	cells	1412:1416	arg1	staining					1442:1449	staining	1442:1449	staining for adhesion protein vinculin	1442:1479	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	8	99	theme	cells	1412:1416	arg1	viability					1399:1407	viability	1399:1407	viability of cells and matrix contraction	1399:1439	However, viability of cells and matrix contraction, staining for adhesion protein vinculin, and hyaluronic acid receptor CD44 remained similar in all CH compositions.
29681215	6	100	from	Changes	1080:1086	arg1	concentrations					1095:1108	HAD concentrations	1091:1108	HAD concentrations	1091:1108	Changes in HAD concentrations altered hydrogel mechanical strength, cytoskeletal organization, and formation of cellular protrusions, all of which contributed to changes in cell morphology and migration.
29681215	11	101	theme	formulations	1875:1886	arg1	importance					1853:1862	the importance	1849:1862	the importance of careful formulations of hydrogel compositions	1849:1911	Our study illustrates the importance of careful formulations of hydrogel compositions in designing biomimetic matrices that are used as in vitro models to study chondrocyte behavior.
29681215	5	102	from	protrusions	961:971	arg1	organization					912:923	The organization	908:923	The organization of the cytoskeleton at the cellular protrusions	908:971	The organization of the cytoskeleton at the cellular protrusions was vimentin localized at the base, microtubules at the tip, and actin localized throughout the cell body.
29681215	1	103	theme	cellular	253:260	arg1	responses					262:270	cellular responses	253:270	cellular responses	253:270	Tissue equivalent collagen-hyaluronic acid-based hydrogels are widely used for cartilage tissue engineering; however, not much importance has been given to investigate how cellular responses are altered with varying concentrations of hyaluronic acid in gels.
31857175	8	0	theme	pure	1182:1185	arg1	BACNF					1187:1191	the pure BACNF	1178:1191	the pure BACNF	1178:1191	The nanocomposite exhibites better thermal stability than the pure BACNF.
31857175	0	1	theme	mechanical	67:76	arg1	properties					105:114	its mechanical, thermal and biodegradable properties	63:114	its mechanical, thermal and biodegradable properties for food packaging	63:133	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	6	2	theme	nanocomposite	934:946	arg1	Morphologies					805:816	Morphologies	805:816	Morphologies	805:816	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	6	2	theme	nanocomposite	934:946	arg1	interactions					851:862	chemical interactions	842:862	chemical interactions	842:862	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	6	2	theme	nanocomposite	934:946	arg1	angles					899:904	water contact angles	885:904	water contact angles	885:904	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	6	2	theme	nanocomposite	934:946	arg1	biodegradability					910:925	biodegradability	910:925	biodegradability	910:925	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	6	2	theme	nanocomposite	934:946	arg1	properties					830:839	mechanical properties	819:839	mechanical properties	819:839	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	6	2	theme	nanocomposite	934:946	arg1	properties					873:882	thermal properties	865:882	thermal properties	865:882	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	2	3	from	biocompatibility	343:358	arg1	conjunction					373:383	conjunction	373:383	conjunction with good mechanical properties of cellulose	373:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	2	4	from	biodegradability	322:337	arg1	conjunction					373:383	conjunction	373:383	conjunction with good mechanical properties of cellulose	373:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	7	5	dep	strength	978:985	arg1	The					966:968	The	966:968	The	966:968	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	0	6	theme	thermal	79:85	arg1	properties					105:114	its mechanical, thermal and biodegradable properties	63:114	its mechanical, thermal and biodegradable properties for food packaging	63:133	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	6	7	theme	mechanical	819:828	arg1	properties					830:839	mechanical properties	819:839	mechanical properties	819:839	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	1	8	theme	chitin	281:286	arg1	materials					306:314	chitin and cellulose raw materials	281:314	chitin and cellulose raw materials	281:314	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	7	9	theme	Young	991:995	arg1	modulus					999:1005	Young's modulus	991:1005	Young's modulus	991:1005	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	1	10	theme	green	157:161	arg1	nanocomposite					163:175	a green nanocomposite	155:175	a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials	155:314	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	0	11	theme	biodegradable	91:103	arg1	properties					105:114	its mechanical, thermal and biodegradable properties	63:114	its mechanical, thermal and biodegradable properties for food packaging	63:133	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	5	12	theme	BACNF	784:788	arg1	concentration					790:802	BACNF concentration	784:802	BACNF concentration	784:802	A simple blending is used to prepare the nanocomposite with different CTNF and BACNF concentration.
31857175	7	13	theme	tensile	970:976	arg1	strength					978:985	tensile strength	970:985	tensile strength	970:985	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	2	14	theme	cellulose	420:428	arg1	properties					406:415	good mechanical properties	390:415	good mechanical properties of cellulose	390:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	2	15	theme	food	495:498	arg1	packaging					500:508	food packaging	495:508	food packaging	495:508	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	7	16	theme	concentration	1096:1108	arg1	increase					1110:1117	the BACNF concentration increase	1086:1117	the BACNF concentration increase	1086:1117	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	3	17	theme	aqueous	604:610	arg1	method					636:641	an aqueous counter collision (ACC) method	601:641	an aqueous counter collision (ACC) method	601:641	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	7	18	dep	3	1053:1053	arg1	to					1050:1051	to	1050:1051	to	1050:1051	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	0	19	theme	Green	0:4	arg1	nanocomposite					6:18	Green nanocomposite	0:18	Green nanocomposite	0:18	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	2	20	theme	applicable	480:489	arg1	nanocomposite					466:478	green nanocomposite	460:478	green nanocomposite applicable for food packaging	460:508	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	1	21	theme	cellulose	292:300	arg1	materials					306:314	chitin and cellulose raw materials	281:314	chitin and cellulose raw materials	281:314	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	0	22	theme	food	120:123	arg1	packaging					125:133	food packaging	120:133	food packaging	120:133	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	10	23	dep	mechanical	1268:1277	arg1	thermal					1280:1286	thermal	1280:1286	thermal	1280:1286	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	2	24	theme	Good	317:320	arg1	biodegradability					322:337	Good biodegradability	317:337	Good biodegradability	317:337	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	2	25	theme	green	460:464	arg1	nanocomposite					466:478	green nanocomposite	460:478	green nanocomposite applicable for food packaging	460:508	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	7	26	theme	nanocomposite	1023:1035	arg1	strength					978:985	tensile strength	970:985	tensile strength	970:985	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	7	26	theme	nanocomposite	1023:1035	arg1	modulus					999:1005	Young's modulus	991:1005	Young's modulus	991:1005	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	1	27	theme	raw	302:304	arg1	materials					306:314	chitin and cellulose raw materials	281:314	chitin and cellulose raw materials	281:314	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	7	28	theme	BACNF	1090:1094	arg1	concentration					1096:1108	the BACNF concentration	1086:1108	the BACNF concentration increase	1086:1117	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	6	29	theme	contact	891:897	arg1	angles					899:904	water contact angles	885:904	water contact angles	885:904	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	3	30	theme	bamboo	515:520	arg1	BACNF					543:547	BACNF	543:547	BACNF	543:547	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	3	30	theme	bamboo	515:520	arg1	nanofiber					532:540	The bamboo cellulose nanofiber	511:540	The bamboo cellulose nanofiber (BACNF)	511:548	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	5	31	used	used	726:729	arg2	blending					714:721	A simple blending	705:721	A simple blending	705:721	A simple blending is used to prepare the nanocomposite with different CTNF and BACNF concentration.
31857175	6	32	theme	water	885:889	arg1	angles					899:904	water contact angles	885:904	water contact angles	885:904	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	3	33	theme	ACC	631:633	arg1	method					636:641	an aqueous counter collision (ACC) method	601:641	an aqueous counter collision (ACC) method	601:641	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	10	34	theme	nanocomposite	1334:1346	arg1	properties					1288:1297	Good mechanical, thermal properties	1263:1297	Good mechanical, thermal properties as well as biodegradability of the nanocomposite	1263:1346	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	10	34	theme	nanocomposite	1334:1346	arg1	biodegradability					1310:1325	biodegradability	1310:1325	Good mechanical, thermal properties as well as biodegradability of the nanocomposite	1263:1346	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	1	35	theme	chitin	201:206	arg1	nanofibers					208:217	chitin nanofibers	201:217	chitin nanofibers	201:217	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	0	36	theme	chitin	30:35	arg1	nanofibers					48:57	chitin and bamboo nanofibers	30:57	nanofibers	48:57	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	3	37	theme	collision	620:628	arg1	method					636:641	an aqueous counter collision (ACC) method	601:641	an aqueous counter collision (ACC) method	601:641	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	10	38	theme	packaging	1380:1388	arg1	application					1390:1400	possible food packaging application	1366:1400	possible food packaging application	1366:1400	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	0	39	theme	bamboo	41:46	arg1	nanofibers					48:57	chitin and bamboo nanofibers	30:57	nanofibers	48:57	Green nanocomposite made with chitin and bamboo nanofibers and its mechanical, thermal and biodegradable properties for food packaging.
31857175	5	40	theme	simple	707:712	arg1	blending					714:721	A simple blending	705:721	A simple blending	705:721	A simple blending is used to prepare the nanocomposite with different CTNF and BACNF concentration.
31857175	6	41	theme	thermal	865:871	arg1	properties					873:882	thermal properties	865:882	thermal properties	865:882	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	10	42	theme	food	1375:1378	arg1	application					1390:1400	possible food packaging application	1366:1400	possible food packaging application	1366:1400	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	2	43	theme	chitin	363:368	arg1	biocompatibility					343:358	biocompatibility	343:358	biocompatibility	343:358	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	2	43	theme	chitin	363:368	arg1	biodegradability					322:337	Good biodegradability	317:337	Good biodegradability	317:337	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	8	44	theme	thermal	1155:1161	arg1	stability					1163:1171	better thermal stability	1148:1171	better thermal stability	1148:1171	The nanocomposite exhibites better thermal stability than the pure BACNF.
31857175	2	45	with	conjunction	373:383	arg1	properties					406:415	good mechanical properties	390:415	good mechanical properties of cellulose	390:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	10	46	theme	possible	1366:1373	arg1	application					1390:1400	possible food packaging application	1366:1400	possible food packaging application	1366:1400	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	3	47	theme	cellulose	522:530	arg1	BACNF					543:547	BACNF	543:547	BACNF	543:547	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	3	47	theme	cellulose	522:530	arg1	nanofiber					532:540	The bamboo cellulose nanofiber	511:540	The bamboo cellulose nanofiber (BACNF)	511:548	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	10	48	theme	mechanical	1268:1277	arg1	properties					1288:1297	Good mechanical, thermal properties	1263:1297	Good mechanical, thermal properties as well as biodegradability of the nanocomposite	1263:1346	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	8	49	theme	better	1148:1153	arg1	stability					1163:1171	better thermal stability	1148:1171	better thermal stability	1148:1171	The nanocomposite exhibites better thermal stability than the pure BACNF.
31857175	7	50	theme	prepared	1014:1021	arg1	nanocomposite					1023:1035	the prepared nanocomposite	1010:1035	the prepared nanocomposite	1010:1035	The tensile strength and Young's modulus of the prepared nanocomposite increased up to 3 and 1.3 times, respectively as the BACNF concentration increase.
31857175	4	51	theme	ACC	693:695	arg1	method					697:702	the ACC method	689:702	the ACC method	689:702	Chitin nanofiber (CTNF) is isolated by using the ACC method.
31857175	10	52	theme	Good	1263:1266	arg1	properties					1288:1297	Good mechanical, thermal properties	1263:1297	Good mechanical, thermal properties as well as biodegradability of the nanocomposite	1263:1346	Good mechanical, thermal properties as well as biodegradability of the nanocomposite are promising for possible food packaging application.
31857175	1	53	theme	bamboo	223:228	arg1	nanofibers					240:249	bamboo cellulose nanofibers	223:249	bamboo cellulose nanofibers	223:249	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	6	54	theme	chemical	842:849	arg1	interactions					851:862	chemical interactions	842:862	chemical interactions	842:862	Morphologies, mechanical properties, chemical interactions, thermal properties, water contact angles and biodegradability of the nanocomposite are investigated.
31857175	3	55	dep	method	636:641	arg1	counter					612:618	counter	612:618	counter	612:618	The bamboo cellulose nanofiber (BACNF) is isolated by using a TEMPO-oxidation followed by an aqueous counter collision (ACC) method.
31857175	2	56	theme	mechanical	395:404	arg1	properties					406:415	good mechanical properties	390:415	good mechanical properties of cellulose	390:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	1	57	theme	cellulose	230:238	arg1	nanofibers					240:249	bamboo cellulose nanofibers	223:249	bamboo cellulose nanofibers	223:249	This paper reports a green nanocomposite made by simply blending chitin nanofibers and bamboo cellulose nanofibers without chemically dissolving chitin and cellulose raw materials.
31857175	5	58	theme	different	765:773	arg1	CTNF					775:778	different CTNF	765:778	different CTNF	765:778	A simple blending is used to prepare the nanocomposite with different CTNF and BACNF concentration.
31857175	2	59	theme	good	390:393	arg1	properties					406:415	good mechanical properties	390:415	good mechanical properties of cellulose	390:428	Good biodegradability and biocompatibility of chitin in conjunction with good mechanical properties of cellulose are beneficial for developing green nanocomposite applicable for food packaging.
31857175	4	60	theme	Chitin	644:649	arg1	CTNF					662:665	CTNF	662:665	CTNF	662:665	Chitin nanofiber (CTNF) is isolated by using the ACC method.
31857175	4	60	theme	Chitin	644:649	arg1	nanofiber					651:659	Chitin nanofiber	644:659	Chitin nanofiber (CTNF)	644:666	Chitin nanofiber (CTNF) is isolated by using the ACC method.
31744759	2	0	theme	use	346:348	arg1	nanocomposites					350:363	new task-specific or general use nanocomposites	317:363	new task-specific or general use nanocomposites	317:363	However, in this way, new task-specific or general use nanocomposites can be obtained.
31744759	1	1	theme	chemical	249:256	arg1	composition					258:268	different size-, shape- or chemical composition	222:268	different size-, shape- or chemical composition	222:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	5	2	theme	association	893:903	arg1	phenomena					926:934	the ordering, sorption, desorption, association and phase separation phenomena	857:934	the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	857:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	6	3	from	process	1005:1011	arg1	interphase					1075:1084	the air-liquid interphase	1060:1084	the air-liquid interphase	1060:1084	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	8	4	theme	prepared	1238:1245	arg1	monolayers					1247:1256	prepared monolayers	1238:1256	prepared monolayers	1238:1256	The stability of prepared monolayers has been studied to investigate the sorption and desorption processes.
31744759	2	5	theme	general	338:344	arg1	nanocomposites					350:363	new task-specific or general use nanocomposites	317:363	new task-specific or general use nanocomposites	317:363	However, in this way, new task-specific or general use nanocomposites can be obtained.
31744759	1	6	theme	composition	258:268	arg1	nanoparticles					205:217	nanoparticles	205:217	nanoparticles of different size-, shape- or chemical composition	205:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	0	7	theme	self-assembly	87:99	arg1	processes					101:109	the self-assembly processes	83:109	the self-assembly processes	83:109	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	5	8	theme	physical	948:955	arg1	properties					970:979	physical and chemical properties	948:979	physical and chemical properties of studied systems	948:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	5	9	theme	chemical	961:968	arg1	properties					970:979	physical and chemical properties	948:979	physical and chemical properties of studied systems	948:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	3	10	theme	different	535:543	arg1	crystals					552:559	different liquid crystals	535:559	different liquid crystals	535:559	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	5	11	theme	phase	909:913	arg1	separation					915:924	phase separation	909:924	phase separation	909:924	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	4	12	theme	Langmuir	677:684	arg1	technique					686:694	the modified Langmuir technique	664:694	the modified Langmuir technique	664:694	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	7	13	theme	molecular	1184:1192	arg1	area					1194:1197	the mean molecular area	1175:1197	the mean molecular area	1175:1197	The compressibility profiles and the mean molecular area have been calculated.
31744759	6	14	theme	different	1102:1110	arg1	compositions					1112:1123	different compositions	1102:1123	different compositions of LC/CNC films	1102:1139	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	5	15	theme	model	762:766	arg1	system					768:773	an excellent model system	749:773	an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	749:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	0	16	theme	processes	101:109	arg1	study					74:78	A study	72:78	A study of the self-assembly processes	72:109	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	0	16	theme	processes	101:109	arg1	stability					125:133	molecular stability	115:133	molecular stability	115:133	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	5	17	theme	desorption	881:890	arg1	phenomena					926:934	the ordering, sorption, desorption, association and phase separation phenomena	857:934	the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	857:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	6	18	theme	self-assembly	1016:1028	arg1	process					1005:1011	The process	1001:1011	The process of self-assembly and molecular organization on the air-liquid interphase	1001:1084	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	8	19	theme	sorption	1294:1301	arg1	processes					1318:1326	the sorption and desorption processes	1290:1326	the sorption and desorption processes	1290:1326	The stability of prepared monolayers has been studied to investigate the sorption and desorption processes.
31744759	3	20	theme	preparation	428:438	arg1	investigations					406:419	the investigations	402:419	the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals	402:559	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	9	21	theme	prepared	1351:1358	arg1	systems					1360:1366	the prepared systems	1347:1366	the prepared systems	1347:1366	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	9	22	theme	systems	1360:1366	arg1	morphology					1333:1342	The morphology	1329:1342	The morphology of the prepared systems	1329:1366	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	5	23	theme	separation	915:924	arg1	phenomena					926:934	the ordering, sorption, desorption, association and phase separation phenomena	857:934	the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	857:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	9	24	theme	LC	1451:1452	arg1	organization					1454:1465	LC organization	1451:1465	LC organization in molecular monolayers	1451:1489	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	5	25	theme	intermolecular	788:801	arg1	interactions					803:814	intermolecular interactions	788:814	intermolecular interactions	788:814	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	1	26	theme	extreme	276:282	arg1	approach					150:157	The bottom-up approach	136:157	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition	136:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	1	26	theme	extreme	276:282	arg1	challenge					284:292	an extreme challenge	273:292	an extreme challenge	273:292	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	1	27	theme	materials	189:197	arg1	synthesis					176:184	and synthesis	172:184	synthesis	176:184	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	0	28	theme	molecular	24:32	arg1	films					34:38	LC molecular films	21:38	LC molecular films	21:38	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	9	29	theme	CNC	1444:1446	arg1	influence					1427:1435	the influence	1423:1435	the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase	1423:1518	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	3	30	theme	liquid	545:550	arg1	crystals					552:559	different liquid crystals	535:559	different liquid crystals	535:559	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	3	31	theme	molecular	455:463	arg1	stability					465:473	molecular stability	455:473	molecular stability	455:473	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	0	32	theme	LC	21:22	arg1	films					34:38	LC molecular films	21:38	LC molecular films	21:38	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	0	33	theme	molecular	115:123	arg1	stability					125:133	molecular stability	115:133	molecular stability	115:133	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	3	34	theme	cellulose	502:510	arg1	CNC					526:528	CNC	526:528	CNC	526:528	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	3	34	theme	cellulose	502:510	arg1	nanocrystals					512:523	cellulose nanocrystals	502:523	cellulose nanocrystals (CNC)	502:529	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	3	35	theme	stability	465:473	arg1	investigations					406:419	the investigations	402:419	the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals	402:559	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	5	36	theme	excellent	752:760	arg1	system					768:773	an excellent model system	749:773	an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	749:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	4	37	theme	modified	668:675	arg1	technique					686:694	the modified Langmuir technique	664:694	the modified Langmuir technique	664:694	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	9	38	theme	air-liquid	1498:1507	arg1	interphase					1509:1518	the air-liquid interphase	1494:1518	the air-liquid interphase	1494:1518	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	6	39	theme	organization	1044:1055	arg1	process					1005:1011	The process	1001:1011	The process of self-assembly and molecular organization on the air-liquid interphase	1001:1084	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	5	40	theme	ordering	861:868	arg1	phenomena					926:934	the ordering, sorption, desorption, association and phase separation phenomena	857:934	the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	857:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	4	41	theme	5PCH	620:623	arg1	crystals					632:639	5PCH liquid crystals	620:639	5PCH liquid crystals	620:639	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	4	41	theme	5PCH	620:623	arg1	5CB					599:601	the 5CB	595:601	the 5CB	595:601	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	0	42	theme	films	34:38	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.	0:134	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	9	43	theme	Brewster	1386:1393	arg1	BAM					1413:1415	BAM	1413:1415	BAM	1413:1415	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	9	43	theme	Brewster	1386:1393	arg1	Microscopy					1401:1410	Brewster Angle Microscopy	1386:1410	Brewster Angle Microscopy (BAM)	1386:1416	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	4	44	theme	CNC-based	566:574	arg1	nanocomposite					576:588	The CNC-based nanocomposite	562:588	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals,	562:640	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	7	45	theme	mean	1179:1182	arg1	area					1194:1197	the mean molecular area	1175:1197	the mean molecular area	1175:1197	The compressibility profiles and the mean molecular area have been calculated.
31744759	1	46	theme	different	222:230	arg1	composition					258:268	different size-, shape- or chemical composition	222:268	different size-, shape- or chemical composition	222:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	7	47	theme	compressibility	1146:1160	arg1	profiles					1162:1169	The compressibility profiles	1142:1169	The compressibility profiles	1142:1169	The compressibility profiles and the mean molecular area have been calculated.
31744759	9	48	theme	Angle	1395:1399	arg1	BAM					1413:1415	BAM	1413:1415	BAM	1413:1415	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	9	48	theme	Angle	1395:1399	arg1	Microscopy					1401:1410	Brewster Angle Microscopy	1386:1410	Brewster Angle Microscopy (BAM)	1386:1416	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	3	49	theme	films	487:491	arg1	preparation					428:438	the preparation procedures and molecular stability	424:473	preparation	428:438	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	3	49	theme	films	487:491	arg1	stability					465:473	molecular stability	455:473	molecular stability	455:473	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	0	50	theme	nanocrystalline	45:59	arg1	cellulose					61:69	nanocrystalline cellulose	45:69	nanocrystalline cellulose	45:69	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	6	51	theme	air-liquid	1064:1073	arg1	interphase					1075:1084	the air-liquid interphase	1060:1084	the air-liquid interphase	1060:1084	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	9	52	from	influence	1427:1435	arg1	interphase					1509:1518	the air-liquid interphase	1494:1518	the air-liquid interphase	1494:1518	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	9	52	from	influence	1427:1435	arg1	organization					1454:1465	LC organization	1451:1465	LC organization in molecular monolayers	1451:1489	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	3	53	dep	preparation	428:438	arg1	procedures					440:449	procedures	440:449	procedures	440:449	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	9	54	theme	molecular	1470:1478	arg1	monolayers					1480:1489	molecular monolayers	1470:1489	molecular monolayers	1470:1489	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	2	55	theme	task-specific	321:333	arg1	nanocomposites					350:363	new task-specific or general use nanocomposites	317:363	new task-specific or general use nanocomposites	317:363	However, in this way, new task-specific or general use nanocomposites can be obtained.
31744759	5	56	theme	studied	984:990	arg1	systems					992:998	studied systems	984:998	studied systems	984:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	0	57	with	Functionalization	0:16	arg1	cellulose					61:69	nanocrystalline cellulose	45:69	nanocrystalline cellulose	45:69	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	2	58	theme	new	317:319	arg1	nanocomposites					350:363	new task-specific or general use nanocomposites	317:363	new task-specific or general use nanocomposites	317:363	However, in this way, new task-specific or general use nanocomposites can be obtained.
31744759	6	59	theme	LC/CNC	1128:1133	arg1	films					1135:1139	LC/CNC films	1128:1139	LC/CNC films	1128:1139	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	1	60	theme	size-	232:236	arg1	composition					258:268	different size-, shape- or chemical composition	222:268	different size-, shape- or chemical composition	222:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	5	61	theme	systems	992:998	arg1	properties					970:979	physical and chemical properties	948:979	physical and chemical properties of studied systems	948:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	3	62	theme	Langmuir	478:485	arg1	films					487:491	Langmuir films	478:491	Langmuir films	478:491	This work presents, the investigations of the preparation procedures and molecular stability of Langmuir films based on cellulose nanocrystals (CNC) and different liquid crystals.
31744759	8	63	theme	monolayers	1247:1256	arg1	stability					1225:1233	The stability	1221:1233	The stability of prepared monolayers	1221:1256	The stability of prepared monolayers has been studied to investigate the sorption and desorption processes.
31744759	6	64	theme	films	1135:1139	arg1	compositions					1112:1123	different compositions	1102:1123	different compositions of LC/CNC films	1102:1139	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	5	65	theme	Langmuir	723:730	arg1	monolayers					732:741	the Langmuir monolayers	719:741	the Langmuir monolayers	719:741	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	6	66	theme	molecular	1034:1042	arg1	organization					1044:1055	molecular organization	1034:1055	molecular organization	1034:1055	The process of self-assembly and molecular organization on the air-liquid interphase was studied for different compositions of LC/CNC films.
31744759	4	67	with	nanocomposite	576:588	arg1	crystals					632:639	5PCH liquid crystals	620:639	5PCH liquid crystals	620:639	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	4	67	with	nanocomposite	576:588	arg1	5FCB					610:613	5FCB	610:613	5FCB	610:613	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	4	67	with	nanocomposite	576:588	arg1	5OCB					604:607	5OCB	604:607	5OCB	604:607	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	4	67	with	nanocomposite	576:588	arg1	5CB					599:601	the 5CB	595:601	the 5CB	595:601	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	9	68	from	organization	1454:1465	arg1	monolayers					1480:1489	molecular monolayers	1470:1489	molecular monolayers	1470:1489	The morphology of the prepared systems was determined by Brewster Angle Microscopy (BAM), and the influence of the CNC on LC organization in molecular monolayers on the air-liquid interphase has been studied.
31744759	1	69	theme	bottom-up	140:148	arg1	approach					150:157	The bottom-up approach	136:157	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition	136:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	1	69	theme	bottom-up	140:148	arg1	challenge					284:292	an extreme challenge	273:292	an extreme challenge	273:292	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	4	70	theme	liquid	625:630	arg1	crystals					632:639	5PCH liquid crystals	620:639	5PCH liquid crystals	620:639	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	4	70	theme	liquid	625:630	arg1	5CB					599:601	the 5CB	595:601	the 5CB	595:601	The CNC-based nanocomposite with the 5CB, 5OCB, 5FCB, and 5PCH liquid crystals, have been obtained by the modified Langmuir technique.
31744759	1	71	theme	shape-	239:244	arg1	composition					258:268	different size-, shape- or chemical composition	222:268	different size-, shape- or chemical composition	222:268	The bottom-up approach in designing and synthesis of materials using nanoparticles of different size-, shape- or chemical composition is an extreme challenge.
31744759	5	72	theme	monolayers	732:741	arg1	investigations					701:714	The investigations	697:714	The investigations of the Langmuir monolayers	697:741	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	0	73	dep	Functionalization	0:16	arg1	study					74:78	A study	72:78	A study of the self-assembly processes	72:109	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	0	73	dep	Functionalization	0:16	arg1	stability					125:133	molecular stability	115:133	molecular stability	115:133	Functionalization of LC molecular films with nanocrystalline cellulose: A study of the self-assembly processes and molecular stability.
31744759	5	74	theme	sorption	871:878	arg1	phenomena					926:934	the ordering, sorption, desorption, association and phase separation phenomena	857:934	the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems	857:998	The investigations of the Langmuir monolayers gives an excellent model system for studying intermolecular interactions at the interface, which allows analyzing the ordering, sorption, desorption, association and phase separation phenomena determining physical and chemical properties of studied systems.
31744759	8	75	theme	desorption	1307:1316	arg1	processes					1318:1326	the sorption and desorption processes	1290:1326	the sorption and desorption processes	1290:1326	The stability of prepared monolayers has been studied to investigate the sorption and desorption processes.
29283560	6	0	theme	particles	1160:1168	arg1	formation					1147:1155	formation	1147:1155	formation of particles	1147:1168	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	4	1	theme	intense	679:685	arg1	effect					698:703	an intense exothermic effect	676:703	an intense exothermic effect	676:703	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	2	2	dep	biomaterial	283:293	arg1	polysaccharides					344:358	polysaccharides	344:358	polysaccharides	344:358	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
29283560	12	3	theme	pH-responsive	2061:2073	arg1	particles					2075:2083	the pH-responsive particles	2057:2083	the pH-responsive particles	2057:2083	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	2	4	theme	natural	307:313	arg1	alginate					322:329	natural source alginate	307:329	natural source alginate	307:329	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
29283560	4	5	theme	thousand	755:762	arg1	kcal/mol					764:771	a thousand kcal/mol	753:771	a thousand kcal/mol	753:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	4	6	from	variation	727:735	arg1	order					744:748	the order	740:748	the order of a thousand kcal/mol	740:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	1	7	theme	environmental	223:235	arg1	applications					237:248	biomedical, biological, and environmental applications	195:248	biomedical, biological, and environmental applications	195:248	Biomaterials conceived for vectorization of bioactives are currently considered for biomedical, biological, and environmental applications.
29283560	12	8	theme	delivery	2200:2207	arg1	development					2166:2176	the development	2162:2176	the development of targeted oral drug delivery	2162:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	5	9	theme	drugs	799:803	arg1	presence					781:788	the presence	777:788	the presence of model drugs mebendazole and ivermectin	777:830	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	10	theme	monomers	934:941	arg1	amount					915:920	the amount	911:920	the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin	911:1023	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	10	theme	monomers	934:941	arg1	monomers					934:941	chitosan monomers	925:941	chitosan monomers	925:941	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	8	11	theme	partial	1395:1401	arg1	hydration					1403:1411	partial hydration	1395:1411	partial hydration	1395:1411	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	1	12	theme	bioactives	155:164	arg1	vectorization					138:150	vectorization	138:150	vectorization of bioactives	138:164	Biomaterials conceived for vectorization of bioactives are currently considered for biomedical, biological, and environmental applications.
29283560	6	13	theme	large	1085:1089	arg1	decrease					1100:1107	large entropic decrease	1085:1107	large entropic decrease	1085:1107	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	12	14	theme	targeted	2181:2188	arg1	delivery					2200:2207	targeted oral drug delivery	2181:2207	targeted oral drug delivery	2181:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	7	15	theme	drug	1324:1327	arg1	presence					1312:1319	the presence	1308:1319	the presence of drug	1308:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	4	16	with	sites	665:669	arg1	effect					698:703	an intense exothermic effect	676:703	an intense exothermic effect	676:703	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	4	16	with	sites	665:669	arg1	variation					727:735	negative enthalpy variation	709:735	negative enthalpy variation	709:735	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	6	17	theme	energy	1074:1079	arg1	state					1042:1046	a state	1040:1046	a state of intense negative Gibbs energy and large entropic decrease	1040:1107	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	9	18	dep	In	1656:1657	arg1	vivo					1659:1662	vivo	1659:1662	vivo	1659:1662	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	7	19	theme	presence	1312:1319	arg1	independent					1293:1303	independent	1293:1303	independent	1293:1303	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	6	20	theme	negative	1059:1066	arg1	energy					1074:1079	intense negative Gibbs energy	1051:1079	intense negative Gibbs energy	1051:1079	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	0	21	theme	Structure	100:108	arg1	Particles					73:81	Biocompatible Particles	59:81	Biocompatible Particles of pH-Responsive Structure	59:108	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	8	22	theme	mild	1608:1611	arg1	mediums					1633:1639	mild and slightly acidic mediums	1608:1639	mild and slightly acidic mediums	1608:1639	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	7	23	theme	aspects	1285:1291	arg1	evidence					1257:1264	further evidence	1249:1264	further evidence of similar surface aspects independent of the presence of drug	1249:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	3	24	theme	composite	431:439	arg1	preparation					450:460	The composite particle preparation	427:460	The composite particle preparation	427:460	The composite particle preparation was in situ monitored by means of isothermal titration calorimetry.
29283560	8	25	theme	surface	1457:1463	arg1	charge					1465:1470	positive surface charge	1448:1470	positive surface charge	1448:1470	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	11	26	theme	substantial	1999:2009	arg1	reduction					2011:2019	substantial reduction	1999:2019	substantial reduction	1999:2019	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	8	27	from	swelling	1596:1603	arg1	mediums					1633:1639	mild and slightly acidic mediums	1608:1639	mild and slightly acidic mediums	1608:1639	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	10	28	theme	oral	1776:1779	arg1	administration					1781:1794	a daily base oral administration	1763:1794	a daily base oral administration	1763:1794	Systematically with a daily base oral administration, particles significantly reduced the infections over 15 days of treatment.
29283560	4	29	theme	effective	647:655	arg1	sites					665:669	0.60 alginate/chitosan effective binding sites	624:669	0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol	624:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	7	30	theme	similar	1269:1275	arg1	aspects					1285:1291	similar surface aspects	1269:1291	similar surface aspects independent of the presence of drug	1269:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	8	31	theme	acid	1482:1485	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	3	32	theme	isothermal	496:505	arg1	calorimetry					517:527	isothermal titration calorimetry	496:527	isothermal titration calorimetry	496:527	The composite particle preparation was in situ monitored by means of isothermal titration calorimetry.
29283560	1	33	theme	biomedical	195:204	arg1	applications					237:248	biomedical, biological, and environmental applications	195:248	biomedical, biological, and environmental applications	195:248	Biomaterials conceived for vectorization of bioactives are currently considered for biomedical, biological, and environmental applications.
29283560	6	34	theme	decrease	1100:1107	arg1	state					1042:1046	a state	1040:1046	a state of intense negative Gibbs energy and large entropic decrease	1040:1107	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	4	35	theme	particle	599:606	arg1	assembly					608:615	particle assembly	599:615	particle assembly	599:615	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	0	36	theme	Biocompatible	59:71	arg1	Particles					73:81	Biocompatible Particles	59:81	Biocompatible Particles of pH-Responsive Structure	59:108	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	0	37	with	Delivery	14:21	arg1	Particles					73:81	Biocompatible Particles	59:81	Biocompatible Particles of pH-Responsive Structure	59:108	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	2	38	theme	delivery	386:393	arg1	system					395:400	a drug delivery system	379:400	a drug delivery system	379:400	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
29283560	7	39	theme	X-ray	1223:1227	arg1	scattering					1229:1238	Small-angle X-ray scattering	1211:1238	Small-angle X-ray scattering	1211:1238	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	8	40	from	predominance	1432:1443	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	9	41	theme	endoparasites	1710:1722	arg1	treatment					1697:1705	the treatment	1693:1705	the treatment of endoparasites in Corydoras fish	1693:1740	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	4	42	theme	exothermic	687:696	arg1	effect					698:703	an intense exothermic effect	676:703	an intense exothermic effect	676:703	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	11	43	theme	infection	2039:2047	arg1	elimination					2024:2034	elimination	2024:2034	elimination of infection	2024:2047	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	11	43	theme	infection	2039:2047	arg1	reduction					2011:2019	substantial reduction	1999:2019	substantial reduction	1999:2019	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	9	44	theme	Corydoras	1727:1735	arg1	fish					1737:1740	Corydoras fish	1727:1740	Corydoras fish	1727:1740	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	4	45	from	effect	698:703	arg1	order					744:748	the order	740:748	the order of a thousand kcal/mol	740:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	0	46	with	Treatment	27:35	arg1	Particles					73:81	Biocompatible Particles	59:81	Biocompatible Particles of pH-Responsive Structure	59:108	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	11	47	theme	drugs	1981:1985	arg1	action					1953:1958	the action	1949:1958	the action of the antiparasitic drugs	1949:1985	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	10	48	theme	treatment	1860:1868	arg1	days					1852:1855	15 days	1849:1855	15 days of treatment	1849:1868	Systematically with a daily base oral administration, particles significantly reduced the infections over 15 days of treatment.
29283560	12	49	theme	prominent	2114:2122	arg1	characteristics					2124:2138	prominent characteristics	2114:2138	prominent characteristics that is promising for the development of targeted oral drug delivery	2114:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	12	50	theme	drug	2195:2198	arg1	delivery					2200:2207	targeted oral drug delivery	2181:2207	targeted oral drug delivery	2181:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	2	51	theme	source	315:320	arg1	alginate					322:329	natural source alginate	307:329	natural source alginate	307:329	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
29283560	5	52	theme	available	943:951	arg1	amount					915:920	the amount	911:920	the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin	911:1023	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	52	theme	available	943:951	arg1	monomers					934:941	chitosan monomers	925:941	chitosan monomers	925:941	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	0	53	theme	Endoparasites	40:52	arg1	Delivery					14:21	Drug Delivery	9:21	Drug Delivery	9:21	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	0	53	theme	Endoparasites	40:52	arg1	Treatment					27:35	Treatment	27:35	Treatment	27:35	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	10	54	theme	daily	1765:1769	arg1	administration					1781:1794	a daily base oral administration	1763:1794	a daily base oral administration	1763:1794	Systematically with a daily base oral administration, particles significantly reduced the infections over 15 days of treatment.
29283560	1	55	theme	biological	207:216	arg1	applications					237:248	biomedical, biological, and environmental applications	195:248	biomedical, biological, and environmental applications	195:248	Biomaterials conceived for vectorization of bioactives are currently considered for biomedical, biological, and environmental applications.
29283560	8	56	theme	physical	1334:1341	arg1	responses					1343:1351	The physical responses	1330:1351	The physical responses of the particles to pH variation	1330:1384	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	4	57	theme	enthalpy	718:725	arg1	variation					727:735	negative enthalpy variation	709:735	negative enthalpy variation	709:735	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	11	58	theme	antiparasitic	1967:1979	arg1	drugs					1981:1985	the antiparasitic drugs	1963:1985	the antiparasitic drugs	1963:1985	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	5	59	theme	model	793:797	arg1	ivermectin					821:830	ivermectin	821:830	ivermectin	821:830	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	59	theme	model	793:797	arg1	mebendazole					805:815	mebendazole	805:815	mebendazole	805:815	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	59	theme	model	793:797	arg1	drugs					799:803	model drugs mebendazole and ivermectin	793:830	model drugs mebendazole and ivermectin	793:830	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	60	theme	chitosan	925:932	arg1	monomers					934:941	chitosan monomers	925:941	chitosan monomers	925:941	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	8	61	theme	particles	1360:1368	arg1	responses					1343:1351	The physical responses	1330:1351	The physical responses of the particles to pH variation	1330:1384	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	11	62	dep	particles	1919:1927	arg1	boosted					1941:1947	boosted	1941:1947	boosted the action of the antiparasitic drugs	1941:1985	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	11	62	dep	particles	1919:1927	arg1	granted					1929:1935	granted	1929:1935	granted	1929:1935	The experiments provide evidence that utilizing particles granted and boosted the action of the antiparasitic drugs, leading to substantial reduction or elimination of infection.
29283560	9	63	from	fish	1737:1740	arg1	treatment					1697:1705	the treatment	1693:1705	the treatment of endoparasites in Corydoras fish	1693:1740	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	8	64	theme	charge	1564:1569	arg1	reversal					1571:1578	charge reversal	1564:1578	charge reversal	1564:1578	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	8	65	theme	pH	1373:1374	arg1	variation					1376:1384	pH variation	1373:1384	pH variation	1373:1384	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	6	66	theme	entropic	1091:1098	arg1	decrease					1100:1107	large entropic decrease	1085:1107	large entropic decrease	1085:1107	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	8	67	attach	predominance	1432:1443	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	8	67	attach	predominance	1432:1443	arg2	charge					1465:1470	positive surface charge	1448:1470	positive surface charge	1448:1470	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	5	68	dep	drugs	799:803	arg1	ivermectin					821:830	ivermectin	821:830	ivermectin	821:830	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	68	dep	drugs	799:803	arg1	mebendazole					805:815	mebendazole	805:815	mebendazole	805:815	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	5	68	dep	drugs	799:803	arg1	drugs					799:803	model drugs mebendazole and ivermectin	793:830	model drugs mebendazole and ivermectin	793:830	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	8	69	theme	new	1592:1594	arg1	swelling					1596:1603	new swelling	1592:1603	compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively	1549:1653	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	4	70	theme	kcal/mol	764:771	arg1	order					744:748	the order	740:748	the order of a thousand kcal/mol	740:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	0	71	theme	pH-Responsive	86:98	arg1	Structure					100:108	pH-Responsive Structure	86:108	pH-Responsive Structure	86:108	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	12	72	with	biomaterial	2097:2107	arg1	characteristics					2124:2138	prominent characteristics	2114:2138	prominent characteristics that is promising for the development of targeted oral drug delivery	2114:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	5	73	theme	small	849:853	arg1	structures					865:874	relatively small and large structures	838:874	relatively small and large structures	838:874	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	6	74	theme	Gibbs	1068:1072	arg1	energy					1074:1079	intense negative Gibbs energy	1051:1079	intense negative Gibbs energy	1051:1079	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	12	75	theme	oral	2190:2193	arg1	delivery					2200:2207	targeted oral drug delivery	2181:2207	targeted oral drug delivery	2181:2207	Hence, the pH-responsive particles represent a biomaterial with prominent characteristics that is promising for the development of targeted oral drug delivery.
29283560	7	76	theme	independent	1293:1303	arg1	aspects					1285:1291	similar surface aspects	1269:1291	similar surface aspects independent of the presence of drug	1269:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	8	77	dep	comprise	1386:1393	arg1	whereas					1495:1501	whereas	1495:1501	whereas	1495:1501	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	6	78	theme	intense	1051:1057	arg1	energy					1074:1079	intense negative Gibbs energy	1051:1079	intense negative Gibbs energy	1051:1079	Nevertheless, a state of intense negative Gibbs energy and large entropic decrease was achieved, providing evidence that formation of particles is thermodynamically driven and favored.
29283560	4	79	theme	strong	534:539	arg1	interaction					541:551	The strong interaction	530:551	The strong interaction established between the macromolecules during particle assembly	530:615	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	0	80	theme	Targeted	0:7	arg1	Delivery					14:21	Drug Delivery	9:21	Drug Delivery	9:21	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	7	81	theme	surface	1277:1283	arg1	aspects					1285:1291	similar surface aspects	1269:1291	similar surface aspects independent of the presence of drug	1269:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	8	82	theme	acidic	1626:1631	arg1	mediums					1633:1639	mild and slightly acidic mediums	1608:1639	mild and slightly acidic mediums	1608:1639	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	0	83	theme	Drug	9:12	arg1	Delivery					14:21	Drug Delivery	9:21	Drug Delivery	9:21	Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure.
29283560	8	84	from	swelling	1414:1421	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	9	85	theme	In	1656:1657	arg1	performance					1664:1674	In vivo performance	1656:1674	In vivo performance	1656:1674	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	9	86	from	endoparasites	1710:1722	arg1	fish					1737:1740	Corydoras fish	1727:1740	Corydoras fish	1727:1740	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	3	87	theme	particle	441:448	arg1	preparation					450:460	The composite particle preparation	427:460	The composite particle preparation	427:460	The composite particle preparation was in situ monitored by means of isothermal titration calorimetry.
29283560	4	88	theme	alginate/chitosan	629:645	arg1	sites					665:669	0.60 alginate/chitosan effective binding sites	624:669	0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol	624:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	8	89	theme	positive	1448:1455	arg1	charge					1465:1470	positive surface charge	1448:1470	positive surface charge	1448:1470	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	7	90	theme	further	1249:1255	arg1	evidence					1257:1264	further evidence	1249:1264	further evidence of similar surface aspects independent of the presence of drug	1249:1327	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	8	91	from	hydration	1403:1411	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	9	92	from	treatment	1697:1705	arg1	fish					1737:1740	Corydoras fish	1727:1740	Corydoras fish	1727:1740	In vivo performance was evaluated in the treatment of endoparasites in Corydoras fish.
29283560	4	93	theme	binding	657:663	arg1	sites					665:669	0.60 alginate/chitosan effective binding sites	624:669	0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol	624:771	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	8	94	theme	charge	1465:1470	arg1	predominance					1432:1443	the predominance	1428:1443	the predominance of positive surface charge in strong acid medium	1428:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	8	94	theme	charge	1465:1470	arg1	swelling					1414:1421	swelling	1414:1421	swelling	1414:1421	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	8	94	theme	charge	1465:1470	arg1	hydration					1403:1411	partial hydration	1395:1411	partial hydration	1395:1411	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	10	95	theme	base	1771:1774	arg1	administration					1781:1794	a daily base oral administration	1763:1794	a daily base oral administration	1763:1794	Systematically with a daily base oral administration, particles significantly reduced the infections over 15 days of treatment.
29283560	5	96	theme	large	859:863	arg1	structures					865:874	relatively small and large structures	838:874	relatively small and large structures	838:874	In the presence of model drugs mebendazole and ivermectin, with relatively small and large structures, respectively, mebendazole reduced the amount of chitosan monomers available to interact with alginate by 27%, which was not observed for ivermectin.
29283560	8	97	theme	strong	1475:1480	arg1	medium					1487:1492	strong acid medium	1475:1492	strong acid medium	1475:1492	The physical responses of the particles to pH variation comprise partial hydration, swelling, and the predominance of positive surface charge in strong acid medium, whereas ionization followed by deprotonation leads to compaction and charge reversal rather than new swelling in mild and slightly acidic mediums, respectively.
29283560	3	98	theme	titration	507:515	arg1	calorimetry					517:527	isothermal titration calorimetry	496:527	isothermal titration calorimetry	496:527	The composite particle preparation was in situ monitored by means of isothermal titration calorimetry.
29283560	7	99	theme	Small-angle	1211:1221	arg1	scattering					1229:1238	Small-angle X-ray scattering	1211:1238	Small-angle X-ray scattering	1211:1238	Small-angle X-ray scattering provided further evidence of similar surface aspects independent of the presence of drug.
29283560	2	100	theme	drug	381:384	arg1	system					395:400	a drug delivery system	379:400	a drug delivery system	379:400	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
29283560	4	101	theme	negative	709:716	arg1	variation					727:735	negative enthalpy variation	709:735	negative enthalpy variation	709:735	The strong interaction established between the macromolecules during particle assembly led to 0.60 alginate/chitosan effective binding sites with an intense exothermic effect and negative enthalpy variation on the order of a thousand kcal/mol.
29283560	2	102	theme	oral	406:409	arg1	administration					411:424	oral administration	406:424	oral administration	406:424	We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration.
31590869	3	0	theme	hydrogen	760:767	arg1	bonds					769:773	hydrogen bonds	760:773	hydrogen bonds	760:773	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	2	1	theme	UV-vis	374:379	arg1	spectroscopy					381:392	UV-vis spectroscopy	374:392	UV-vis spectroscopy	374:392	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	3	2	with	interactions	743:754	arg1	ZnO-NPs					780:786	ZnO-NPs	780:786	ZnO-NPs	780:786	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	5	3	with	nanofibers	1117:1126	arg1	distribution					1140:1151	a good distribution	1133:1151	a good distribution of the used nanofillers	1133:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	4	theme	electrostatic	729:741	arg1	interactions					743:754	electrostatic interactions	729:754	electrostatic interactions	729:754	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	4	5	theme	thermogravimetric	954:970	arg1	analysis					972:979	thermogravimetric analysis	954:979	thermogravimetric analysis	954:979	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	5	6	theme	EDX	1024:1026	arg1	spectroscopy					1028:1039	EDX spectroscopy	1024:1039	EDX spectroscopy	1024:1039	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	4	7	theme	stable	857:862	arg1	products					864:871	stable products	857:871	stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis	857:979	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	4	8	theme	washing-crosslinking	803:822	arg1	protocol					824:831	An innovative washing-crosslinking protocol	789:831	An innovative washing-crosslinking protocol	789:831	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	4	9	theme	innovative	792:801	arg1	protocol					824:831	An innovative washing-crosslinking protocol	789:831	An innovative washing-crosslinking protocol	789:831	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	5	10	theme	several	1219:1225	arg1	purposes					1227:1234	several purposes	1219:1234	several purposes (e.g. biomedical, pharmaceutical and environmental applications)	1219:1299	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	10	theme	several	1219:1225	arg1	applications					1287:1298	biomedical, pharmaceutical and environmental applications	1242:1298	biomedical, pharmaceutical and environmental applications	1242:1298	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	11	theme	homogenous	1106:1115	arg1	nanofibers					1117:1126	thin homogenous nanofibers	1101:1126	thin homogenous nanofibers with a good distribution of the used nanofillers	1101:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	2	12	theme	stabilizing	330:340	arg1	agent					342:346	stabilizing agent	330:346	stabilizing agent	330:346	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	4	13	theme	InfraRed	928:935	arg1	spectroscopy					937:948	InfraRed spectroscopy	928:948	InfraRed spectroscopy	928:948	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	3	14	contain	containing	454:463	arg1	Formulations					441:452	Formulations	441:452	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs	441:513	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	14	contain	containing	454:463	arg2	oxide					496:500	ethylene oxide	487:500	ethylene oxide	487:500	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	14	contain	containing	454:463	arg2	poly					482:485	poly	482:485	poly(ethylene oxide)	482:501	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	14	contain	containing	454:463	arg2	ZnO-NPs					507:513	ZnO-NPs	507:513	ZnO-NPs	507:513	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	14	contain	containing	454:463	arg2	alginate					472:479	sodium alginate	465:479	sodium alginate	465:479	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	2	15	theme	sol-gel	287:293	arg1	method					295:300	a "green" sol-gel method	277:300	a "green" sol-gel method	277:300	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	2	16	theme	"	285:285	arg1	method					295:300	a "green" sol-gel method	277:300	a "green" sol-gel method	277:300	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	2	17	theme	morphological	417:429	arg1	analysis					431:438	morphological analysis	417:438	morphological analysis	417:438	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	5	18	dep	applications	1287:1298	arg1	e.g.					1237:1240	e.g.	1237:1240	e.g.	1237:1240	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	19	theme	alginate	596:603	arg1	structure					615:623	alginate molecular structure	596:623	alginate molecular structure	596:623	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	5	20	theme	pharmaceutical	1254:1267	arg1	applications					1287:1298	biomedical, pharmaceutical and environmental applications	1242:1298	biomedical, pharmaceutical and environmental applications	1242:1298	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	20	theme	pharmaceutical	1254:1267	arg1	purposes					1227:1234	several purposes	1219:1234	several purposes (e.g. biomedical, pharmaceutical and environmental applications)	1219:1299	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	1	21	theme	present	100:106	arg1	work					108:111	the present work	96:111	the present work	96:111	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	5	22	theme	Morphological	982:994	arg1	investigation					996:1008	Morphological investigation	982:1008	Morphological investigation combined with EDX spectroscopy	982:1039	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	0	23	theme	alginate-based	25:38	arg1	membranes					52:60	composite alginate-based electrospun membranes	15:60	composite alginate-based electrospun membranes	15:60	Preparation of composite alginate-based electrospun membranes loaded with ZnO nanoparticles.
31590869	5	24	theme	obtained	1052:1059	arg1	mats					1061:1064	the obtained mats	1048:1064	the obtained mats	1048:1064	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	4	25	dep	Fourier	910:916	arg1	Transform					918:926	Transform	918:926	Transform InfraRed spectroscopy and thermogravimetric analysis	918:979	An innovative washing-crosslinking protocol was developed to obtain stable products which composition was assessed using Fourier Transform InfraRed spectroscopy and thermogravimetric analysis.
31590869	0	26	theme	composite	15:23	arg1	membranes					52:60	composite alginate-based electrospun membranes	15:60	composite alginate-based electrospun membranes	15:60	Preparation of composite alginate-based electrospun membranes loaded with ZnO nanoparticles.
31590869	3	27	theme	suitable	563:570	arg1	ones					572:575	the most suitable ones	554:575	the most suitable ones	554:575	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	1	28	theme	alginate-based	113:126	arg1	membranes					140:148	alginate-based nanofibrous membranes	113:148	alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs)	113:193	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	0	29	theme	membranes	52:60	arg1	Preparation					0:10	Preparation	0:10	Preparation of composite alginate-based electrospun membranes	0:60	Preparation of composite alginate-based electrospun membranes loaded with ZnO nanoparticles.
31590869	5	30	theme	potential	1196:1204	arg1	products					1206:1213	potential products	1196:1213	potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications)	1196:1299	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	31	theme	good	1135:1138	arg1	distribution					1140:1151	a good distribution	1133:1151	a good distribution of the used nanofillers	1133:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	32	theme	ethylene	487:494	arg1	poly					482:485	poly	482:485	poly(ethylene oxide)	482:501	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	32	theme	ethylene	487:494	arg1	oxide					496:500	ethylene oxide	487:500	ethylene oxide	487:500	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	0	33	theme	electrospun	40:50	arg1	membranes					52:60	composite alginate-based electrospun membranes	15:60	composite alginate-based electrospun membranes	15:60	Preparation of composite alginate-based electrospun membranes loaded with ZnO nanoparticles.
31590869	5	34	theme	environmental	1273:1285	arg1	applications					1287:1298	biomedical, pharmaceutical and environmental applications	1242:1298	biomedical, pharmaceutical and environmental applications	1242:1298	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	34	theme	environmental	1273:1285	arg1	purposes					1227:1234	several purposes	1219:1234	several purposes (e.g. biomedical, pharmaceutical and environmental applications)	1219:1299	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	35	theme	sodium	465:470	arg1	alginate					472:479	sodium alginate	465:479	sodium alginate	465:479	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	36	theme	important	635:643	arg1	role					645:648	an important role	632:648	an important role	632:648	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	37	theme	due	679:681	arg1	spinnability					666:677	the solution spinnability	653:677	the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs	653:786	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	1	38	theme	nanofibrous	128:138	arg1	membranes					140:148	alginate-based nanofibrous membranes	113:148	alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs)	113:193	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	5	39	theme	used	1160:1163	arg1	nanofillers					1165:1175	the used nanofillers	1156:1175	the used nanofillers	1156:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	40	with	bonds	769:773	arg1	ZnO-NPs					780:786	ZnO-NPs	780:786	ZnO-NPs	780:786	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	5	41	theme	nanofillers	1165:1175	arg1	distribution					1140:1151	a good distribution	1133:1151	a good distribution of the used nanofillers	1133:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	2	42	theme	green	280:284	arg1	method					295:300	a "green" sol-gel method	277:300	a "green" sol-gel method	277:300	ZnO-NPs were synthesized by means of a "green" sol-gel method by using alginate itself as stabilizing agent and characterized through UV-vis spectroscopy, thermogravimetric and morphological analysis.
31590869	5	43	theme	thin	1101:1104	arg1	nanofibers					1117:1126	thin homogenous nanofibers	1101:1126	thin homogenous nanofibers with a good distribution of the used nanofillers	1101:1175	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	44	theme	polysaccharide	690:703	arg1	capability					705:714	the polysaccharide capability	686:714	the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs	686:786	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	3	45	theme	solution	657:664	arg1	spinnability					666:677	the solution spinnability	653:677	the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs	653:786	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
31590869	1	46	theme	zinc	160:163	arg1	oxide					165:169	zinc oxide	160:169	zinc oxide nanoparticles (ZnO-NPs)	160:193	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	1	47	theme	oxide	165:169	arg1	nanoparticles					171:183	zinc oxide nanoparticles	160:183	zinc oxide nanoparticles (ZnO-NPs)	160:193	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	1	47	theme	oxide	165:169	arg1	ZnO-NPs					186:192	ZnO-NPs	186:192	ZnO-NPs	186:192	In the present work alginate-based nanofibrous membranes embedding zinc oxide nanoparticles (ZnO-NPs) were prepared via electrospinning technique.
31590869	0	48	theme	ZnO	74:76	arg1	nanoparticles					78:90	ZnO nanoparticles	74:90	ZnO nanoparticles	74:90	Preparation of composite alginate-based electrospun membranes loaded with ZnO nanoparticles.
31590869	5	49	theme	biomedical	1242:1251	arg1	applications					1287:1298	biomedical, pharmaceutical and environmental applications	1242:1298	biomedical, pharmaceutical and environmental applications	1242:1298	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	5	49	theme	biomedical	1242:1251	arg1	purposes					1227:1234	several purposes	1219:1234	several purposes (e.g. biomedical, pharmaceutical and environmental applications)	1219:1299	Morphological investigation combined with EDX spectroscopy proved the obtained mats were highly porous and composed by thin homogenous nanofibers with a good distribution of the used nanofillers, thus representing potential products for several purposes (e.g. biomedical, pharmaceutical and environmental applications).
31590869	3	50	theme	molecular	605:613	arg1	structure					615:623	alginate molecular structure	596:623	alginate molecular structure	596:623	Formulations containing sodium alginate, poly(ethylene oxide) and ZnO-NPs were rheologically studied to identify the most suitable ones to be electrospun; alginate molecular structure played an important role on the solution spinnability due to the polysaccharide capability to establish electrostatic interactions and hydrogen bonds with ZnO-NPs.
29745989	3	0	theme	dialysis	463:470	arg1	GDR					485:487	GDR	485:487	GDR	485:487	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	0	theme	dialysis	463:470	arg1	retardation					472:482	glucose dialysis retardation	455:482	glucose dialysis retardation (GDR) of the solid fraction	455:510	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	12	1	theme	processed	1854:1862	arg1	guava					1864:1868	processed guava	1854:1868	processed guava	1854:1868	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	1	2	theme	waste	87:91	arg1	valorization					65:76	The valorization	61:76	The valorization of guava waste	61:91	The valorization of guava waste requires compositional and functional studies.
29745989	5	3	theme	molecular	826:834	arg1	weight					836:841	low molecular weight	822:841	low molecular weight carbohydrates	822:855	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	10	4	theme	in	1567:1568	arg1	study					1575:1579	further in vivo study	1559:1579	further in vivo study	1559:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	4	5	theme	deoxycholic	772:782	arg1	acid					784:787	deoxycholic acid	772:787	deoxycholic acid	772:787	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	6	theme	7.7	620:622	arg1	%					623:623	%	623:623	%	623:623	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	10	7	theme	guava	1450:1454	arg1	byproducts					1456:1465	All three guava byproducts	1440:1465	All three guava byproducts	1440:1465	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	6	8	theme	scavenging	1063:1072	arg1	capacity					1074:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	9	9	theme	phytate	1424:1430	arg1	levels					1432:1437	the high phytate levels	1415:1437	the high phytate levels	1415:1437	The refiner waste could be included in animal feed but requires further processing to reduce the high phytate levels.
29745989	3	10	theme	fraction	503:510	arg1	WHC					379:381	WHC	379:381	WHC	379:381	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	OHC					398:400	OHC	398:400	OHC	398:400	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	retardation					472:482	glucose dialysis retardation	455:482	glucose dialysis retardation (GDR) of the solid fraction	455:510	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	binding					442:448	bile acid binding	432:448	bile acid binding	432:448	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	capacities					559:568	the antioxidative and α-amylase inhibitory capacities	516:568	capacities	559:568	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	GDR					485:487	GDR	485:487	GDR	485:487	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	holding					389:395	oil holding	385:395	oil holding (OHC)	385:401	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	capacities					420:429	cation exchange capacities	404:429	cation exchange capacities	404:429	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	10	theme	fraction	503:510	arg1	holding					370:376	the water holding	360:376	the water holding (WHC)	360:382	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	9	11	theme	refiner	1326:1332	arg1	waste					1334:1338	The refiner waste	1322:1338	The refiner waste	1322:1338	The refiner waste could be included in animal feed but requires further processing to reduce the high phytate levels.
29745989	9	12	theme	high	1419:1422	arg1	levels					1432:1437	the high phytate levels	1415:1437	the high phytate levels	1415:1437	The refiner waste could be included in animal feed but requires further processing to reduce the high phytate levels.
29745989	8	13	theme	GDR	1309:1311	arg1	acid					1269:1272	cholic acid	1262:1272	cholic acid (86.6%)	1262:1280	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	13	theme	GDR	1309:1311	arg1	%					1279:1279	86.6%	1275:1279	86.6%	1275:1279	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	13	theme	GDR	1309:1311	arg1	values					1299:1304	the highest values	1287:1304	the highest values of GDR and AIC	1287:1319	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	14	theme	decanter	1182:1189	arg1	values					1172:1177	The WHC and OHC values	1156:1177	The WHC and OHC values of decanter	1156:1189	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	4	15	contain	had	688:690	arg1	it					685:686	it	685:686	it	685:686	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	15	contain	had	688:690	arg2	score					718:722	a high prebiotic activity score	692:722	a high prebiotic activity score	692:722	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	3	16	theme	inhibitory	548:557	arg1	capacities					559:568	the antioxidative and α-amylase inhibitory capacities	516:568	capacities	559:568	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	12	17	theme	marketable	1810:1819	arg1	products					1821:1828	other marketable products	1804:1828	other marketable products	1804:1828	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	8	18	theme	AIC	1317:1319	arg1	acid					1269:1272	cholic acid	1262:1272	cholic acid (86.6%)	1262:1280	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	18	theme	AIC	1317:1319	arg1	%					1279:1279	86.6%	1275:1279	86.6%	1275:1279	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	18	theme	AIC	1317:1319	arg1	values					1299:1304	the highest values	1287:1304	the highest values of GDR and AIC	1287:1319	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	12	19	theme	better	1934:1939	arg1	use					1941:1943	better use	1934:1943	better use of them	1934:1951	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	5	20	theme	weight	836:841	arg1	carbohydrates					843:855	low molecular weight carbohydrates	822:855	low molecular weight carbohydrates	822:855	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	3	21	theme	prebiotic	288:296	arg1	score					307:311	the prebiotic activity score	284:311	the prebiotic activity score	284:311	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	8	22	theme	narrow	1205:1210	arg1	range					1212:1216	a narrow range	1203:1216	a narrow range	1203:1216	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	9	23	theme	animal	1361:1366	arg1	feed					1368:1371	animal feed	1361:1371	animal feed	1361:1371	The refiner waste could be included in animal feed but requires further processing to reduce the high phytate levels.
29745989	6	24	theme	highest	940:946	arg1	binding					948:954	the highest binding	936:954	the highest binding	936:954	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	11	25	theme	binding	1680:1686	arg1	properties					1688:1697	useful physicochemical binding properties	1657:1697	useful physicochemical binding properties	1657:1697	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	3	26	theme	selected	317:324	arg1	carbohydrates					326:338	selected carbohydrates	317:338	selected carbohydrates	317:338	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	4	27	theme	7.08	632:635	arg1	%					636:636	%	636:636	%	636:636	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	7	28	theme	prebiotic	1130:1138	arg1	score					1149:1153	a high prebiotic activity score	1123:1153	a high prebiotic activity score	1123:1153	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	5	29	theme	low	822:824	arg1	weight					836:841	low molecular weight	822:841	low molecular weight carbohydrates	822:855	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	8	30	theme	highest	1241:1247	arg1	binding					1249:1255	the highest binding	1237:1255	the highest binding	1237:1255	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	11	31	theme	PRACTICAL	1582:1590	arg1	APPLICATION					1592:1602	PRACTICAL APPLICATION	1582:1602	PRACTICAL APPLICATION To differing extents, the guava byproducts	1582:1645	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	5	32	theme	crude	893:897	arg1	fiber					899:903	relatively low crude fiber and cellulose contents	878:926	fiber	899:903	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	0	33	from	Processing	49:58	arg1	Byproducts					21:30	Potential Functional Byproducts	0:30	Potential Functional Byproducts from Guava Purée Processing	0:58	Potential Functional Byproducts from Guava Purée Processing.
29745989	10	34	theme	fiber	1523:1527	arg1	source					1493:1498	a source	1491:1498	a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study	1491:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	1	35	theme	guava	81:85	arg1	waste					87:91	guava waste	81:91	guava waste	81:91	The valorization of guava waste requires compositional and functional studies.
29745989	4	36	theme	high	663:666	arg1	content					676:682	a relatively high phytate content	650:682	a relatively high phytate content	650:682	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	11	37	theme	health-promoting	1734:1749	arg1	activity					1751:1758	health-promoting activity	1734:1758	health-promoting activity	1734:1758	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	0	38	theme	Potential	0:8	arg1	Byproducts					21:30	Potential Functional Byproducts	0:30	Potential Functional Byproducts from Guava Purée Processing	0:58	Potential Functional Byproducts from Guava Purée Processing.
29745989	5	39	theme	tannin	867:872	arg1	levels					812:817	high levels	807:817	high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents	807:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	2	40	theme	processing	182:191	arg1	decanter					222:229	decanter	222:229	decanter	222:229	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	2	40	theme	processing	182:191	arg1	refiner					201:207	refiner	201:207	refiner	201:207	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	2	40	theme	processing	182:191	arg1	byproducts					156:165	three byproducts	150:165	three byproducts	150:165	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	2	40	theme	processing	182:191	arg1	siever					210:215	siever	210:215	siever	210:215	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	11	41	contain	possessed	1706:1714	arg1	APPLICATION					1592:1602	PRACTICAL APPLICATION	1582:1602	PRACTICAL APPLICATION To differing extents, the guava byproducts	1582:1645	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	11	41	contain	possessed	1706:1714	arg2	potential					1720:1728	the potential	1716:1728	the potential for health-promoting activity	1716:1758	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	3	42	theme	extract	593:599	arg1	antioxidative					520:532	antioxidative	520:532	antioxidative	520:532	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	42	theme	extract	593:599	arg1	AIC					571:573	AIC	571:573	AIC	571:573	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	43	theme	exchange	411:418	arg1	capacities					420:429	cation exchange capacities	404:429	cation exchange capacities	404:429	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	0	44	theme	Guava	37:41	arg1	Processing					49:58	Guava Purée Processing	37:58	Guava Purée Processing	37:58	Potential Functional Byproducts from Guava Purée Processing.
29745989	2	45	theme	guava	170:174	arg1	processing					182:191	guava purée processing	170:191	guava purée processing	170:191	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	4	46	theme	highest	742:748	arg1	capacity					758:765	the highest binding capacity	738:765	the highest binding capacity	738:765	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	47	theme	binding	750:756	arg1	capacity					758:765	the highest binding capacity	738:765	the highest binding capacity	738:765	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	6	48	theme	DPPH	1049:1052	arg1	capacity					1074:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	8	49	theme	highest	1291:1297	arg1	values					1299:1304	the highest values	1287:1304	the highest values of GDR and AIC	1287:1319	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	7	50	contain	had	1119:1121	arg2	score					1149:1153	a high prebiotic activity score	1123:1153	a high prebiotic activity score	1123:1153	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	7	50	contain	had	1119:1121	arg1	Decanter					1084:1091	Decanter	1084:1091	Decanter	1084:1091	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	3	51	theme	acid	437:440	arg1	binding					442:448	bile acid binding	432:448	bile acid binding	432:448	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	6	52	theme	2,2-diphenyl-1-picrylhydrazyl	1018:1046	arg1	capacity					1074:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	10	53	theme	further	1559:1565	arg1	study					1575:1579	further in vivo study	1559:1579	further in vivo study	1559:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	3	54	theme	glucose	455:461	arg1	GDR					485:487	GDR	485:487	GDR	485:487	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	54	theme	glucose	455:461	arg1	retardation					472:482	glucose dialysis retardation	455:482	glucose dialysis retardation (GDR) of the solid fraction	455:510	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	12	55	theme	guava	1864:1868	arg1	able					1879:1882	able	1879:1882	able	1879:1882	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	12	55	theme	guava	1864:1868	arg1	manufacturers					1837:1849	the manufacturers	1833:1849	the manufacturers of processed guava	1833:1868	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	10	56	theme	dietary	1515:1521	arg1	DF					1530:1531	DF	1530:1531	DF	1530:1531	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	10	56	theme	dietary	1515:1521	arg1	fiber					1523:1527	antioxidant dietary fiber	1503:1527	antioxidant dietary fiber (DF)	1503:1532	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	10	56	theme	dietary	1515:1521	arg1	finding					1537:1543	a finding	1535:1543	a finding that warrants further in vivo study	1535:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	3	57	theme	chemical	248:255	arg1	composition					257:267	the chemical composition	244:267	the chemical composition	244:267	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	8	58	theme	WHC	1160:1162	arg1	values					1172:1177	The WHC and OHC values	1156:1177	The WHC and OHC values of decanter	1156:1189	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	11	59	theme	guava	1630:1634	arg1	extents					1617:1623	differing extents	1607:1623	differing extents	1607:1623	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	11	59	theme	guava	1630:1634	arg1	byproducts					1636:1645	the guava byproducts	1626:1645	the guava byproducts	1626:1645	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	3	60	theme	solid	497:501	arg1	fraction					503:510	the solid fraction	493:510	the solid fraction	493:510	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	7	61	from	cellulose	1105:1113	arg1	rich					1097:1100	rich	1097:1100	rich	1097:1100	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	9	62	theme	further	1386:1392	arg1	processing					1394:1403	further processing	1386:1403	further processing	1386:1403	The refiner waste could be included in animal feed but requires further processing to reduce the high phytate levels.
29745989	8	63	theme	OHC	1168:1170	arg1	values					1172:1177	The WHC and OHC values	1156:1177	The WHC and OHC values of decanter	1156:1189	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	3	64	theme	α-amylase	538:546	arg1	capacities					559:568	the antioxidative and α-amylase inhibitory capacities	516:568	capacities	559:568	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	11	65	theme	physicochemical	1664:1678	arg1	properties					1688:1697	useful physicochemical binding properties	1657:1697	useful physicochemical binding properties	1657:1697	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	1	66	theme	functional	120:129	arg1	studies					131:137	compositional and functional studies	102:137	compositional and functional studies	102:137	The valorization of guava waste requires compositional and functional studies.
29745989	12	67	theme	other	1804:1808	arg1	products					1821:1828	other marketable products	1804:1828	other marketable products	1804:1828	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	10	68	dep	in	1567:1568	arg1	vivo					1570:1573	vivo	1570:1573	vivo	1570:1573	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	4	69	theme	%	636:636	arg1	protein					638:644	7.08% protein	632:644	7.08% protein	632:644	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	5	70	theme	cellulose	909:917	arg1	contents					919:926	relatively low crude fiber and cellulose contents	878:926	contents	919:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	5	71	theme	carbohydrates	843:855	arg1	levels					812:817	high levels	807:817	high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents	807:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	3	72	theme	activity	298:305	arg1	score					307:311	the prebiotic activity score	284:311	the prebiotic activity score	284:311	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	6	73	contain	had	932:934	arg2	binding					948:954	the highest binding	936:954	the highest binding	936:954	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	6	73	contain	had	932:934	arg1	It					929:930	It	929:930	It	929:930	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	4	74	theme	prebiotic	699:707	arg1	score					718:722	a high prebiotic activity score	692:722	a high prebiotic activity score	692:722	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	7	75	theme	activity	1140:1147	arg1	score					1149:1153	a high prebiotic activity score	1123:1153	a high prebiotic activity score	1123:1153	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	5	76	theme	high	807:810	arg1	levels					812:817	high levels	807:817	high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents	807:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	4	77	theme	%	623:623	arg1	lipid					625:629	7.7% lipid	620:629	7.7% lipid	620:629	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	7	78	theme	high	1125:1128	arg1	score					1149:1153	a high prebiotic activity score	1123:1153	a high prebiotic activity score	1123:1153	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	5	79	contain	contained	797:805	arg1	Siever					790:795	Siever	790:795	Siever	790:795	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	5	79	contain	contained	797:805	arg2	levels					812:817	high levels	807:817	high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents	807:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	11	80	theme	differing	1607:1615	arg1	extents					1617:1623	differing extents	1607:1623	differing extents	1607:1623	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	11	80	theme	differing	1607:1615	arg1	byproducts					1636:1645	the guava byproducts	1626:1645	the guava byproducts	1626:1645	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	5	81	theme	low	889:891	arg1	fiber					899:903	relatively low crude fiber and cellulose contents	878:926	fiber	899:903	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	6	82	theme	radical	1055:1061	arg1	capacity					1074:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	3	83	theme	water	364:368	arg1	WHC					379:381	WHC	379:381	WHC	379:381	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	83	theme	water	364:368	arg1	holding					370:376	the water holding	360:376	the water holding (WHC)	360:382	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	4	84	contain	possessed	728:736	arg1	it					685:686	it	685:686	it	685:686	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	84	contain	possessed	728:736	arg2	capacity					758:765	the highest binding capacity	738:765	the highest binding capacity	738:765	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	12	85	theme	them	1948:1951	arg1	use					1941:1943	better use	1934:1943	better use of them	1934:1951	These byproducts could also be upgraded to other marketable products so the manufacturers of processed guava might be able to develop their businesses sustainably by making better use of them.
29745989	5	86	theme	fiber	899:903	arg1	levels					812:817	high levels	807:817	high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents	807:926	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	7	87	from	rich	1097:1100	arg1	cellulose					1105:1113	cellulose	1105:1113	cellulose	1105:1113	Decanter was rich in cellulose and had a high prebiotic activity score.
29745989	3	88	theme	cation	404:409	arg1	capacities					420:429	cation exchange capacities	404:429	cation exchange capacities	404:429	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	0	89	theme	Functional	10:19	arg1	Byproducts					21:30	Potential Functional Byproducts	0:30	Potential Functional Byproducts from Guava Purée Processing	0:58	Potential Functional Byproducts from Guava Purée Processing.
29745989	5	90	theme	total	861:865	arg1	tannin					867:872	total tannin	861:872	total tannin	861:872	Siever contained high levels of low molecular weight carbohydrates and total tannin but relatively low crude fiber and cellulose contents.
29745989	8	91	theme	cholic	1262:1267	arg1	acid					1269:1272	cholic acid	1262:1272	cholic acid (86.6%)	1262:1280	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	8	91	theme	cholic	1262:1267	arg1	%					1279:1279	86.6%	1275:1279	86.6%	1275:1279	The WHC and OHC values of decanter were within a narrow range and also exhibited the highest binding with cholic acid (86.6%), and the highest values of GDR and AIC.
29745989	1	92	theme	compositional	102:114	arg1	studies					131:137	compositional and functional studies	102:137	compositional and functional studies	102:137	The valorization of guava waste requires compositional and functional studies.
29745989	3	93	theme	ethanolic	583:591	arg1	extract					593:599	the ethanolic extract	579:599	the ethanolic extract	579:599	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	4	94	theme	phytate	668:674	arg1	content					676:682	a relatively high phytate content	650:682	a relatively high phytate content	650:682	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	3	95	theme	bile	432:435	arg1	binding					442:448	bile acid binding	432:448	bile acid binding	432:448	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	0	96	theme	Purée	43:47	arg1	Processing					49:58	Guava Purée Processing	37:58	Guava Purée Processing	37:58	Potential Functional Byproducts from Guava Purée Processing.
29745989	6	97	theme	chenodeoxycholic	961:976	arg1	acid					978:981	chenodeoxycholic acid	961:981	chenodeoxycholic acid (74.8%)	961:989	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	6	97	theme	chenodeoxycholic	961:976	arg1	%					988:988	74.8%	984:988	74.8%	984:988	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	2	98	theme	purée	176:180	arg1	processing					182:191	guava purée processing	170:191	guava purée processing	170:191	We tested three byproducts of guava purée processing, namely refiner, siever, and decanter.
29745989	10	99	theme	antioxidant	1503:1513	arg1	DF					1530:1531	DF	1530:1531	DF	1530:1531	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	10	99	theme	antioxidant	1503:1513	arg1	fiber					1523:1527	antioxidant dietary fiber	1503:1527	antioxidant dietary fiber (DF)	1503:1532	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	10	99	theme	antioxidant	1503:1513	arg1	finding					1537:1543	a finding	1535:1543	a finding that warrants further in vivo study	1535:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	11	100	theme	useful	1657:1662	arg1	properties					1688:1697	useful physicochemical binding properties	1657:1697	useful physicochemical binding properties	1657:1697	PRACTICAL APPLICATION To differing extents, the guava byproducts exhibited useful physicochemical binding properties and so possessed the potential for health-promoting activity.
29745989	3	101	theme	oil	385:387	arg1	OHC					398:400	OHC	398:400	OHC	398:400	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	3	101	theme	oil	385:387	arg1	holding					389:395	oil holding	385:395	oil holding (OHC)	385:401	We analyzed the chemical composition and quantified the prebiotic activity score and selected carbohydrates; we also determined the water holding (WHC), oil holding (OHC), cation exchange capacities, bile acid binding, and glucose dialysis retardation (GDR) of the solid fraction and the antioxidative and α-amylase inhibitory capacities (AIC) of the ethanolic extract.
29745989	4	102	contain	contained	610:618	arg1	Refiner					602:608	Refiner	602:608	Refiner	602:608	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	102	contain	contained	610:618	arg2	protein					638:644	7.08% protein	632:644	7.08% protein	632:644	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	102	contain	contained	610:618	arg2	content					676:682	a relatively high phytate content	650:682	a relatively high phytate content	650:682	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	4	102	contain	contained	610:618	arg2	lipid					625:629	7.7% lipid	620:629	7.7% lipid	620:629	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	10	103	contain	had	1467:1469	arg2	potential					1475:1483	the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study	1471:1579	the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study	1471:1579	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	10	103	contain	had	1467:1469	arg1	byproducts					1456:1465	All three guava byproducts	1440:1465	All three guava byproducts	1440:1465	All three guava byproducts had the potential to be a source of antioxidant dietary fiber (DF), a finding that warrants further in vivo study.
29745989	4	104	theme	high	694:697	arg1	score					718:722	a high prebiotic activity score	692:722	a high prebiotic activity score	692:722	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
29745989	6	105	theme	highest	1010:1016	arg1	capacity					1074:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity	1006:1081	It had the highest binding with chenodeoxycholic acid (74.8%), and exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity.
29745989	4	106	theme	activity	709:716	arg1	score					718:722	a high prebiotic activity score	692:722	a high prebiotic activity score	692:722	Refiner contained 7.7% lipid, 7.08% protein and a relatively high phytate content; it had a high prebiotic activity score and possessed the highest binding capacity with deoxycholic acid.
30543888	0	0	theme	extended-release	73:88	arg1	formulations					90:101	extended-release formulations	73:101	extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics	73:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	1	1	dep	in	286:287	arg1	vivo					289:292	vivo	289:292	vivo	289:292	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	4	2	theme	formulations	1099:1110	arg1	profiles					1071:1078	the pharmacokinetic profiles	1051:1078	the pharmacokinetic profiles of the baclofen ER formulations	1051:1110	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	6	3	theme	ER	1506:1507	arg1	formulations					1509:1520	ER formulations	1506:1520	ER formulations	1506:1520	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	5	4	theme	IVIVC	1172:1176	arg1	model					1178:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	4	5	dep	in	948:949	arg1	vitro					951:955	vitro	951:955	vitro	951:955	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	2	6	theme	given	613:617	arg1	composition					619:629	a given composition	611:629	a given composition of the formulation	611:648	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	5	7	theme	in	1231:1232	arg1	dissolution					1240:1250	the in vitro dissolution	1227:1250	the in vitro dissolution	1227:1250	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	4	8	theme	in	885:886	arg1	parameters					907:916	the corresponding in vitro dissolutions parameters	867:916	the corresponding in vitro dissolutions parameters	867:916	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	2	9	theme	drug	548:551	arg1	concentration					553:565	the plasma drug concentration	537:565	the plasma drug concentration	537:565	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	4	10	dep	in	1005:1006	arg1	vivo					1008:1011	vivo	1008:1011	vivo	1008:1011	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	4	11	theme	in	1005:1006	arg1	parameters					1025:1034	the in vivo dissolution parameters	1001:1034	the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling	1001:1150	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	1	12	theme	in	257:258	arg1	dissolution					266:276	the in vitro dissolution	253:276	the in vitro dissolution	253:276	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	0	13	theme	formulations	90:101	arg1	development					58:68	the development	54:68	the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics	54:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	2	14	theme	plasma	541:546	arg1	concentration					553:565	the plasma drug concentration	537:565	the plasma drug concentration	537:565	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	3	15	with	tablets	711:717	arg1	rates					746:750	different dissolution rates	724:750	different dissolution rates	724:750	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	5	16	theme	plasma	1269:1274	arg1	profile					1290:1296	the in vivo plasma concentration profile	1257:1296	the in vivo plasma concentration profile	1257:1296	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	4	17	theme	baclofen	1087:1094	arg1	formulations					1099:1110	the baclofen ER formulations	1083:1110	the baclofen ER formulations	1083:1110	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	5	18	dep	in	1349:1350	arg1	vivo					1352:1355	vivo	1352:1355	vivo	1352:1355	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	1	19	theme	new	352:354	arg1	formulations					378:389	new extended release (ER) formulations	352:389	new extended release (ER) formulations	352:389	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	2	20	theme	drug	586:589	arg1	concentrations					591:604	plasma drug concentrations	579:604	plasma drug concentrations from a given composition of the formulation	579:648	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	5	21	theme	ER	1410:1411	arg1	formulations					1413:1424	baclofen ER formulations	1401:1424	baclofen ER formulations	1401:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	6	22	theme	formulations	1509:1520	arg1	compositions					1490:1501	optimal compositions	1482:1501	optimal compositions of ER formulations	1482:1520	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	2	23	dep	in	511:512	arg1	vitro					514:518	vitro	514:518	vitro	514:518	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	1	24	theme	extended	356:363	arg1	formulations					378:389	new extended release (ER) formulations	352:389	new extended release (ER) formulations	352:389	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	2	25	theme	IVIVC	439:443	arg1	approach					445:452	the IVIVC approach	435:452	the IVIVC approach	435:452	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	2	26	theme	formulation	638:648	arg1	composition					619:629	a given composition	611:629	a given composition of the formulation	611:648	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	0	27	from	composition	134:144	arg1	baclofen					107:114	baclofen:	107:115	baclofen: From formulation composition to in vivo pharmacokinetics	107:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	2	28	theme	model	687:691	arg1	drug					693:696	a model drug	685:696	a model drug	685:696	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	2	28	theme	model	687:691	arg1	baclofen					673:680	baclofen	673:680	baclofen	673:680	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	1	29	theme	vitro-in	178:185	arg1	IVIVC					205:209	IVIVC	205:209	IVIVC	205:209	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	1	29	theme	vitro-in	178:185	arg1	correlation					192:202	vitro-in vivo correlation	178:202	vitro-in vivo correlation (IVIVC)	178:210	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	1	30	theme	release	365:371	arg1	formulations					378:389	new extended release (ER) formulations	352:389	new extended release (ER) formulations	352:389	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	6	31	theme	concentration	1548:1560	arg1	profile					1562:1568	desired plasma concentration profile	1533:1568	desired plasma concentration profile	1533:1568	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	4	32	theme	ER	1096:1097	arg1	formulations					1099:1110	the baclofen ER formulations	1083:1110	the baclofen ER formulations	1083:1110	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	1	33	theme	vivo	187:190	arg1	IVIVC					205:209	IVIVC	205:209	IVIVC	205:209	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	1	33	theme	vivo	187:190	arg1	correlation					192:202	vitro-in vivo correlation	178:202	vitro-in vivo correlation (IVIVC)	178:210	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	2	34	theme	study	415:419	arg1	aim					396:398	The aim	392:398	The aim of the present study	392:419	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	6	35	theme	great	1454:1458	arg1	promise					1460:1466	great promise	1454:1466	great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile	1454:1568	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	4	36	theme	dissolution	957:967	arg1	parameters					969:978	the in vitro dissolution parameters	944:978	the in vitro dissolution parameters	944:978	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	4	37	theme	pharmacokinetic	1055:1069	arg1	profiles					1071:1078	the pharmacokinetic profiles	1051:1078	the pharmacokinetic profiles of the baclofen ER formulations	1051:1110	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	0	38	theme	vitro-in	18:25	arg1	model					44:48	vitro-in vivo correlation model	18:48	vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics	18:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	3	39	theme	different	724:732	arg1	rates					746:750	different dissolution rates	724:750	different dissolution rates	724:750	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	0	40	theme	formulation	122:132	arg1	composition					134:144	formulation composition	122:144	formulation composition	122:144	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	5	41	theme	baclofen	1401:1408	arg1	formulations					1413:1424	baclofen ER formulations	1401:1424	baclofen ER formulations	1401:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	42	theme	HPMC	1393:1396	arg1	amount					1383:1388	the amount	1379:1388	the amount of HPMC in baclofen ER formulations	1379:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	42	theme	HPMC	1393:1396	arg1	HPMC					1393:1396	HPMC	1393:1396	HPMC	1393:1396	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	43	theme	final	1157:1161	arg1	model					1178:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	2	44	theme	in	511:512	arg1	dissolution					520:530	the in vitro dissolution	507:530	the in vitro dissolution	507:530	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	4	45	theme	pharmacokinetic	1127:1141	arg1	modeling					1143:1150	population pharmacokinetic modeling	1116:1150	population pharmacokinetic modeling	1116:1150	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	5	46	theme	in	1261:1262	arg1	profile					1290:1296	the in vivo plasma concentration profile	1257:1296	the in vivo plasma concentration profile	1257:1296	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	0	47	theme	correlation	32:42	arg1	model					44:48	vitro-in vivo correlation model	18:48	vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics	18:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	1	48	theme	in	286:287	arg1	pharmacokinetics					294:309	the in vivo pharmacokinetics	282:309	the in vivo pharmacokinetics	282:309	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	4	49	theme	corresponding	871:883	arg1	parameters					907:916	the corresponding in vitro dissolutions parameters	867:916	the corresponding in vitro dissolutions parameters	867:916	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	5	50	theme	in	1349:1350	arg1	pharmacokinetics					1357:1372	in vivo pharmacokinetics	1349:1372	in vivo pharmacokinetics	1349:1372	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	51	theme	concentration	1276:1288	arg1	profile					1290:1296	the in vivo plasma concentration profile	1257:1296	the in vivo plasma concentration profile	1257:1296	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	4	52	theme	dissolutions	894:905	arg1	parameters					907:916	the corresponding in vitro dissolutions parameters	867:916	the corresponding in vitro dissolutions parameters	867:916	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	4	53	dep	in	885:886	arg1	vitro					888:892	vitro	888:892	vitro	888:892	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	6	54	theme	desired	1533:1539	arg1	profile					1562:1568	desired plasma concentration profile	1533:1568	desired plasma concentration profile	1533:1568	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	2	55	theme	present	407:413	arg1	study					415:419	the present study	403:419	the present study	403:419	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	5	56	theme	extended	1163:1170	arg1	model					1178:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model	1153:1182	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	3	57	theme	ER	708:709	arg1	tablets					711:717	Baclofen ER tablets	699:717	Baclofen ER tablets with different dissolution rates	699:750	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	1	58	theme	formulations	378:389	arg1	development					337:347	the development	333:347	the development of new extended release (ER) formulations	333:389	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	5	59	theme	formulation	1214:1224	arg1	dissolution					1240:1250	the in vitro dissolution	1227:1250	the in vitro dissolution	1227:1250	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	59	theme	formulation	1214:1224	arg1	profile					1290:1296	the in vivo plasma concentration profile	1257:1296	the in vivo plasma concentration profile	1257:1296	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	59	theme	formulation	1214:1224	arg1	composition					1195:1205	the composition	1191:1205	the composition of the formulation	1191:1224	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	60	from	amount	1383:1388	arg1	formulations					1413:1424	baclofen ER formulations	1401:1424	baclofen ER formulations	1401:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	5	60	from	amount	1383:1388	arg1	prediction					1335:1344	the prediction	1331:1344	the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations	1331:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	4	61	theme	in	948:949	arg1	parameters					969:978	the in vitro dissolution parameters	944:978	the in vitro dissolution parameters	944:978	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	3	62	theme	Baclofen	699:706	arg1	tablets					711:717	Baclofen ER tablets	699:717	Baclofen ER tablets with different dissolution rates	699:750	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	0	63	theme	in	149:150	arg1	pharmacokinetics					157:172	in vivo pharmacokinetics	149:172	in vivo pharmacokinetics	149:172	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	1	64	dep	in	257:258	arg1	vitro					260:264	vitro	260:264	vitro	260:264	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	2	65	theme	plasma	579:584	arg1	concentrations					591:604	plasma drug concentrations	579:604	plasma drug concentrations from a given composition of the formulation	579:648	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	3	66	theme	hydroxypropyl	796:808	arg1	HPMC					827:830	HPMC	827:830	HPMC	827:830	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	3	66	theme	hydroxypropyl	796:808	arg1	methylcellulose					810:824	hydroxypropyl methylcellulose	796:824	hydroxypropyl methylcellulose (HPMC)	796:831	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	1	67	used	utilized	320:327	arg2	model					239:243	a predictive mathematical model	213:243	a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics	213:309	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	4	68	theme	HPMC	845:848	arg1	compositions					850:861	the HPMC compositions	841:861	the HPMC compositions	841:861	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	5	69	dep	in	1231:1232	arg1	vitro					1234:1238	vitro	1234:1238	vitro	1234:1238	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	0	70	dep	vitro-in	18:25	arg1	vivo					27:30	vivo	27:30	vivo	27:30	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	5	71	theme	pharmacokinetics	1357:1372	arg1	prediction					1335:1344	the prediction	1331:1344	the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations	1331:1424	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	3	72	theme	methylcellulose	810:824	arg1	composition					781:791	the composition	777:791	the composition of hydroxypropyl methylcellulose (HPMC)	777:831	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	4	73	theme	population	1116:1125	arg1	modeling					1143:1150	population pharmacokinetic modeling	1116:1150	population pharmacokinetic modeling	1116:1150	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	0	74	dep	in	149:150	arg1	vivo					152:155	vivo	152:155	vivo	152:155	Novel extended in vitro-in vivo correlation model for the development of extended-release formulations for baclofen: From formulation composition to in vivo pharmacokinetics.
30543888	1	75	theme	predictive	215:224	arg1	model					239:243	a predictive mathematical model	213:243	a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics	213:309	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	6	76	theme	plasma	1541:1546	arg1	profile					1562:1568	desired plasma concentration profile	1533:1568	desired plasma concentration profile	1533:1568	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	6	77	theme	optimal	1482:1488	arg1	compositions					1490:1501	optimal compositions	1482:1501	optimal compositions of ER formulations	1482:1520	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	4	78	theme	dissolution	1013:1023	arg1	parameters					1025:1034	the in vivo dissolution parameters	1001:1034	the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling	1001:1150	First, the HPMC compositions and the corresponding in vitro dissolutions parameters were correlated, and then the in vitro dissolution parameters were correlated with the in vivo dissolution parameters extracted from the pharmacokinetic profiles of the baclofen ER formulations via population pharmacokinetic modeling.
30543888	1	79	theme	mathematical	226:237	arg1	model					239:243	a predictive mathematical model	213:243	a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics	213:309	In vitro-in vivo correlation (IVIVC), a predictive mathematical model between the in vitro dissolution and the in vivo pharmacokinetics has been utilized for the development of new extended release (ER) formulations.
30543888	3	80	theme	dissolution	734:744	arg1	rates					746:750	different dissolution rates	724:750	different dissolution rates	724:750	Baclofen ER tablets with different dissolution rates were prepared by varying the composition of hydroxypropyl methylcellulose (HPMC).
30543888	5	81	dep	in	1261:1262	arg1	vivo					1264:1267	vivo	1264:1267	vivo	1264:1267	The final extended IVIVC model linked the composition of the formulation, the in vitro dissolution, and the in vivo plasma concentration profile and was successfully applied for the prediction of in vivo pharmacokinetics from the amount of HPMC in baclofen ER formulations.
30543888	2	82	from	composition	619:629	arg1	concentrations					591:604	plasma drug concentrations	579:604	plasma drug concentrations from a given composition of the formulation	579:648	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30543888	6	83	theme	present	1431:1437	arg1	approach					1439:1446	The present approach	1427:1446	The present approach	1427:1446	The present approach holds great promise for designing optimal compositions of ER formulations to present desired plasma concentration profile.
30543888	2	84	theme	formulation	482:492	arg1	composition					494:504	the formulation composition	478:504	the formulation composition	478:504	The aim of the present study was to extend the IVIVC approach, which correlates among the formulation composition, the in vitro dissolution, and the plasma drug concentration, to predict plasma drug concentrations from a given composition of the formulation, and vice versa, using baclofen as a model drug.
30630786	0	0	theme	arthritis	109:117	arg1	rat					119:121	rheumatoid arthritis rat	98:121	rheumatoid arthritis rat	98:121	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	5	1	theme	analytic	648:655	arg1	cytometry					662:670	analytic flow cytometry	648:670	analytic flow cytometry	648:670	The cell viability was evaluated by MTT assay and the apoptosis was determined by analytic flow cytometry.
30630786	12	2	dep	inhibit	1442:1448	arg1	suggested					1549:1557	suggested	1549:1557	suggested a potential therapeutic value for rheumatoid arthritis	1549:1612	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	0	3	theme	rheumatoid	98:107	arg1	arthritis					109:117	rheumatoid arthritis	98:117	rheumatoid arthritis rat	98:121	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	12	4	theme	inflammatory	1521:1532	arg1	factors					1534:1540	inflammatory factors	1521:1540	inflammatory factors	1521:1540	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	5	5	theme	flow	657:660	arg1	cytometry					662:670	analytic flow cytometry	648:670	analytic flow cytometry	648:670	The cell viability was evaluated by MTT assay and the apoptosis was determined by analytic flow cytometry.
30630786	1	6	from	effect	171:176	arg1	FLS					260:262	FLS	260:262	FLS	260:262	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	1	6	from	effect	171:176	arg1	synoviocytes					246:257	fibroblast-like synoviocytes	230:257	fibroblast-like synoviocytes (FLS)	230:263	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	2	7	theme	composite	375:383	arg1	factors					385:391	the classical composite factors	361:391	the classical composite factors including wind, cold, damp plus biological agents	361:441	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	7	theme	composite	375:383	arg1	damp					415:418	damp	415:418	damp	415:418	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	7	theme	composite	375:383	arg1	agents					436:441	biological agents	425:441	biological agents	425:441	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	7	theme	composite	375:383	arg1	cold					409:412	cold	409:412	cold	409:412	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	7	theme	composite	375:383	arg1	wind					403:406	wind	403:406	wind	403:406	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	10	8	theme	apoptotic	1264:1272	arg1	protein					1291:1297	apoptotic marker caspase-3 protein	1264:1297	apoptotic marker caspase-3 protein	1264:1297	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	10	9	theme	protein	1291:1297	arg1	level					1209:1213	level	1209:1213	level	1209:1213	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	10	9	theme	protein	1291:1297	arg1	ratio					1199:1203	the Bcl-2/Bax ratio	1185:1203	the Bcl-2/Bax ratio	1185:1203	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	2	10	theme	Rheumatoid	311:320	arg1	arthritis					322:330	Rheumatoid arthritis	311:330	Rheumatoid arthritis rat model	311:340	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	1	11	theme	rheumatoid	279:288	arg1	arthritis					290:298	rheumatoid arthritis	279:298	rheumatoid arthritis rat model	279:308	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	12	12	theme	therapeutic	1571:1581	arg1	value					1583:1587	a potential therapeutic value	1559:1587	a potential therapeutic value for rheumatoid arthritis	1559:1612	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	10	13	theme	MMP-1	1218:1222	arg1	level					1209:1213	level	1209:1213	level	1209:1213	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	10	13	theme	MMP-1	1218:1222	arg1	ratio					1199:1203	the Bcl-2/Bax ratio	1185:1203	the Bcl-2/Bax ratio	1185:1203	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	12	14	theme	rheumatoid	1593:1602	arg1	arthritis					1604:1612	rheumatoid arthritis	1593:1612	rheumatoid arthritis	1593:1612	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	1	15	theme	arthritis	290:298	arg1	model					304:308	rheumatoid arthritis rat model	279:308	rheumatoid arthritis rat model	279:308	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	7	16	dep	factors	780:786	arg1	TNF-α					788:792	TNF-α	788:792	TNF-α	788:792	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	16	dep	factors	780:786	arg1	factors					780:786	secretory inflammation factors TNF-α and IL-1β	757:802	secretory inflammation factors TNF-α and IL-1β	757:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	16	dep	factors	780:786	arg1	IL-1β					798:802	IL-1β	798:802	IL-1β	798:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	9	17	theme	TNF-α	1134:1138	arg1	secretion					1121:1129	the secretion	1117:1129	the secretion of TNF-α (P<0.05) and IL-1β (P<0.05)	1117:1166	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	9	18	theme	P53	1055:1057	arg1	expression					1041:1050	the expression	1037:1050	the expression of P53	1037:1057	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	1	19	theme	Saposhnikovia	181:193	arg1	polysaccharide					206:219	Saposhnikovia divaricata polysaccharide	181:219	Saposhnikovia divaricata polysaccharide (SDP)	181:225	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	1	19	theme	Saposhnikovia	181:193	arg1	SDP					222:224	SDP	222:224	SDP	222:224	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	12	20	theme	RAFLS	1463:1467	arg1	dysplasia					1450:1458	dysplasia	1450:1458	dysplasia of RAFLS	1450:1467	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	0	21	from	polysaccharide	46:59	arg1	synoviocytes					80:91	fibroblast-like synoviocytes	64:91	fibroblast-like synoviocytes	64:91	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	6	22	theme	gene	702:705	arg1	change					688:693	The expression change	673:693	The expression change of p53 gene	673:705	The expression change of p53 gene was monitored by RT-PCR method.
30630786	1	23	theme	divaricata	195:204	arg1	polysaccharide					206:219	Saposhnikovia divaricata polysaccharide	181:219	Saposhnikovia divaricata polysaccharide (SDP)	181:225	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	1	23	theme	divaricata	195:204	arg1	SDP					222:224	SDP	222:224	SDP	222:224	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	6	24	theme	p53	698:700	arg1	gene					702:705	p53 gene	698:705	p53 gene	698:705	The expression change of p53 gene was monitored by RT-PCR method.
30630786	1	25	theme	polysaccharide	206:219	arg1	effect					171:176	inhibitory effect	160:176	inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS)	160:263	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	0	26	theme	Inhibitory	0:9	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of Saposhnikovia	0:33	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	1	27	theme	rat	300:302	arg1	model					304:308	rheumatoid arthritis rat model	279:308	rheumatoid arthritis rat model	279:308	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	9	28	theme	mRNA	1067:1070	arg1	level					1102:1106	both mRNA (P<0.05) and protein (P<0.05) level	1062:1106	both mRNA (P<0.05) and protein (P<0.05) level	1062:1106	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	3	29	theme	synovial	448:455	arg1	tissues					457:463	The synovial tissues	444:463	The synovial tissues	444:463	The synovial tissues were digested with trypsin to isolate FLS cells.
30630786	0	30	theme	Saposhnikovia	21:33	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of Saposhnikovia	0:33	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	12	31	theme	factors	1534:1540	arg1	suppression					1506:1516	suppression	1506:1516	suppression of inflammatory factors	1506:1540	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	12	31	theme	factors	1534:1540	arg1	modulation					1473:1482	modulation	1473:1482	modulation of p53 expression	1473:1500	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	9	32	dep	enhance	1029:1035	arg1	inhibit					1109:1115	inhibit	1109:1115	inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05)	1109:1166	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	2	33	theme	rat	332:334	arg1	model					336:340	Rheumatoid arthritis rat model	311:340	Rheumatoid arthritis rat model	311:340	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	5	34	theme	MTT	602:604	arg1	assay					606:610	MTT assay	602:610	MTT assay	602:610	The cell viability was evaluated by MTT assay and the apoptosis was determined by analytic flow cytometry.
30630786	9	35	theme	IL-1β	1153:1157	arg1	secretion					1121:1129	the secretion	1117:1129	the secretion of TNF-α (P<0.05) and IL-1β (P<0.05)	1117:1166	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	2	36	theme	arthritis	322:330	arg1	model					336:340	Rheumatoid arthritis rat model	311:340	Rheumatoid arthritis rat model	311:340	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	6	37	theme	expression	677:686	arg1	change					688:693	The expression change	673:693	The expression change of p53 gene	673:705	The expression change of p53 gene was monitored by RT-PCR method.
30630786	10	38	theme	MMP-3	1225:1229	arg1	level					1209:1213	level	1209:1213	level	1209:1213	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	10	38	theme	MMP-3	1225:1229	arg1	ratio					1199:1203	the Bcl-2/Bax ratio	1185:1203	the Bcl-2/Bax ratio	1185:1203	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	11	39	theme	apoptosis-inducing	1375:1392	arg1	effect					1394:1399	the apoptosis-inducing effect	1371:1399	the apoptosis-inducing effect of SDP on RAFLS	1371:1415	In addition, the FACS analysis consistently consolidated the apoptosis-inducing effect of SDP on RAFLS.
30630786	2	40	theme	biological	425:434	arg1	agents					436:441	biological agents	425:441	biological agents	425:441	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	11	41	theme	FACS	1331:1334	arg1	analysis					1336:1343	the FACS analysis	1327:1343	the FACS analysis	1327:1343	In addition, the FACS analysis consistently consolidated the apoptosis-inducing effect of SDP on RAFLS.
30630786	11	42	theme	SDP	1404:1406	arg1	effect					1394:1399	the apoptosis-inducing effect	1371:1399	the apoptosis-inducing effect of SDP on RAFLS	1371:1415	In addition, the FACS analysis consistently consolidated the apoptosis-inducing effect of SDP on RAFLS.
30630786	1	43	theme	fibroblast-like	230:244	arg1	FLS					260:262	FLS	260:262	FLS	260:262	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	1	43	theme	fibroblast-like	230:244	arg1	synoviocytes					246:257	fibroblast-like synoviocytes	230:257	fibroblast-like synoviocytes (FLS)	230:263	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	0	44	theme	fibroblast-like	64:78	arg1	synoviocytes					80:91	fibroblast-like synoviocytes	64:91	fibroblast-like synoviocytes	64:91	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	4	45	theme	SDP	538:540	arg1	dosage					528:533	The different dosage	514:533	The different dosage of SDP	514:540	The different dosage of SDP was applied in culture.
30630786	12	46	theme	potential	1561:1569	arg1	value					1583:1587	a potential therapeutic value	1559:1587	a potential therapeutic value for rheumatoid arthritis	1559:1612	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	11	47	from	effect	1394:1399	arg1	RAFLS					1411:1415	RAFLS	1411:1415	RAFLS	1411:1415	In addition, the FACS analysis consistently consolidated the apoptosis-inducing effect of SDP on RAFLS.
30630786	9	48	theme	protein	1085:1091	arg1	level					1102:1106	both mRNA (P<0.05) and protein (P<0.05) level	1062:1106	both mRNA (P<0.05) and protein (P<0.05) level	1062:1106	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	2	49	theme	classical	365:373	arg1	factors					385:391	the classical composite factors	361:391	the classical composite factors including wind, cold, damp plus biological agents	361:441	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	49	theme	classical	365:373	arg1	damp					415:418	damp	415:418	damp	415:418	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	49	theme	classical	365:373	arg1	agents					436:441	biological agents	425:441	biological agents	425:441	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	49	theme	classical	365:373	arg1	cold					409:412	cold	409:412	cold	409:412	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	2	49	theme	classical	365:373	arg1	wind					403:406	wind	403:406	wind	403:406	Rheumatoid arthritis rat model was established by the classical composite factors including wind, cold, damp plus biological agents.
30630786	7	50	theme	inflammation	767:778	arg1	TNF-α					788:792	TNF-α	788:792	TNF-α	788:792	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	50	theme	inflammation	767:778	arg1	factors					780:786	secretory inflammation factors TNF-α and IL-1β	757:802	secretory inflammation factors TNF-α and IL-1β	757:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	50	theme	inflammation	767:778	arg1	IL-1β					798:802	IL-1β	798:802	IL-1β	798:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	9	51	theme	high	997:1000	arg1	concentration					1002:1014	high concentration	997:1014	high concentration of SDP	997:1021	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	7	52	theme	factors	780:786	arg1	production					743:752	The production	739:752	The production of secretory inflammation factors TNF-α and IL-1β	739:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	10	53	theme	Bcl-2/Bax	1189:1197	arg1	ratio					1199:1203	the Bcl-2/Bax ratio	1185:1203	the Bcl-2/Bax ratio	1185:1203	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	5	54	theme	cell	570:573	arg1	viability					575:583	The cell viability	566:583	The cell viability	566:583	The cell viability was evaluated by MTT assay and the apoptosis was determined by analytic flow cytometry.
30630786	8	55	theme	western	937:943	arg1	blotting					945:952	western blotting	937:952	western blotting	937:952	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53 were measured by western blotting.
30630786	9	56	with	treatment	982:990	arg1	concentration					1002:1014	high concentration	997:1014	high concentration of SDP	997:1021	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	10	57	theme	caspase-3	1281:1289	arg1	protein					1291:1297	apoptotic marker caspase-3 protein	1264:1297	apoptotic marker caspase-3 protein	1264:1297	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	3	58	theme	FLS	503:505	arg1	cells					507:511	FLS cells	503:511	FLS cells	503:511	The synovial tissues were digested with trypsin to isolate FLS cells.
30630786	8	59	theme	proliferative	834:846	arg1	proteins					862:869	The proliferative and apoptotic proteins	830:869	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53	830:918	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53 were measured by western blotting.
30630786	8	59	theme	proliferative	834:846	arg1	Bcl-2					879:883	Bcl-2	879:883	Bcl-2	879:883	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53 were measured by western blotting.
30630786	7	60	theme	secretory	757:765	arg1	TNF-α					788:792	TNF-α	788:792	TNF-α	788:792	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	60	theme	secretory	757:765	arg1	factors					780:786	secretory inflammation factors TNF-α and IL-1β	757:802	secretory inflammation factors TNF-α and IL-1β	757:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	7	60	theme	secretory	757:765	arg1	IL-1β					798:802	IL-1β	798:802	IL-1β	798:802	The production of secretory inflammation factors TNF-α and IL-1β were determined by ELISA.
30630786	0	61	from	rat	119:121	arg1	polysaccharide					46:59	polysaccharide	46:59	polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat	46:121	Inhibitory effect of Saposhnikovia divaricate polysaccharide on fibroblast-like synoviocytes from rheumatoid arthritis rat in vitro.
30630786	10	62	theme	marker	1274:1279	arg1	protein					1291:1297	apoptotic marker caspase-3 protein	1264:1297	apoptotic marker caspase-3 protein	1264:1297	Simultaneously, the Bcl-2/Bax ratio and level of MMP-1, MMP-3 was significantly decreased, and apoptotic marker caspase-3 protein was increased.
30630786	9	63	theme	SDP	1019:1021	arg1	concentration					1002:1014	high concentration	997:1014	high concentration of SDP	997:1021	Our data demonstrated that treatment with high concentration of SDP could enhance the expression of P53 at both mRNA (P<0.05) and protein (P<0.05) level, inhibit the secretion of TNF-α (P<0.05) and IL-1β (P<0.05).
30630786	12	64	theme	expression	1491:1500	arg1	suppression					1506:1516	suppression	1506:1516	suppression of inflammatory factors	1506:1540	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	12	64	theme	expression	1491:1500	arg1	modulation					1473:1482	modulation	1473:1482	modulation of p53 expression	1473:1500	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30630786	4	65	theme	different	518:526	arg1	dosage					528:533	The different dosage	514:533	The different dosage of SDP	514:540	The different dosage of SDP was applied in culture.
30630786	6	66	theme	RT-PCR	724:729	arg1	method					731:736	RT-PCR method	724:736	RT-PCR method	724:736	The expression change of p53 gene was monitored by RT-PCR method.
30630786	1	67	theme	inhibitory	160:169	arg1	effect					171:176	inhibitory effect	160:176	inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS)	160:263	To study the mechanism and inhibitory effect of Saposhnikovia divaricata polysaccharide (SDP) on fibroblast-like synoviocytes (FLS) isolated from rheumatoid arthritis rat model.
30630786	8	68	theme	apoptotic	852:860	arg1	proteins					862:869	The proliferative and apoptotic proteins	830:869	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53	830:918	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53 were measured by western blotting.
30630786	8	68	theme	apoptotic	852:860	arg1	Bcl-2					879:883	Bcl-2	879:883	Bcl-2	879:883	The proliferative and apoptotic proteins such as Bcl-2, Bax, Caspase-3, MMP-1, MMP-3, P53 were measured by western blotting.
30630786	12	69	theme	p53	1487:1489	arg1	expression					1491:1500	p53 expression	1487:1500	p53 expression	1487:1500	SDP could significantly inhibit dysplasia of RAFLS via modulation of p53 expression and suppression of inflammatory factors, which suggested a potential therapeutic value for rheumatoid arthritis.
30372996	2	0	theme	different	496:504	arg1	women					506:510	different women	496:510	different women	496:510	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	0	1	theme	electrospray	183:194	arg1	spectrometry					214:225	electrospray ionization - mass spectrometry	183:225	electrospray ionization - mass spectrometry	183:225	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	2	2	theme	Secretor	558:565	arg1	phenotypes					577:586	the Secretor and Lewis phenotypes	554:586	phenotypes	577:586	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	4	3	theme	ionization-mass	939:953	arg1	spectrometry					955:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	1	4	from	development	407:417	arg1	infants					422:428	infants	422:428	infants	422:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	2	5	theme	HMOs	591:594	arg1	biosynthesis					596:607	HMOs biosynthesis	591:607	HMOs biosynthesis	591:607	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	5	6	from	HMOs	1018:1021	arg1	milk					1036:1039	the human milk	1026:1039	the human milk obtained from 10 women	1026:1062	This method was validated and applied to analyze HMOs in the human milk obtained from 10 women.
30372996	4	7	theme	graphitized	885:895	arg1	spectrometry					955:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	0	8	theme	neutral	90:96	arg1	oligosaccharides					120:135	16 neutral and acidic human milk oligosaccharides	87:135	16 neutral and acidic human milk oligosaccharides	87:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	1	9	theme	pathogen	344:351	arg1	binding					353:359	pathogen binding	344:359	pathogen binding	344:359	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	10	from	milk	307:310	arg1	present					290:296	present	290:296	present	290:296	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	11	attach	present	290:296	arg2	glycans					272:278	free glycans	267:278	free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants	267:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	11	attach	present	290:296	arg2	oligosaccharides					239:254	Human milk oligosaccharides	228:254	Human milk oligosaccharides (HMOs)	228:261	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	11	attach	present	290:296	arg1	milk					307:310	human milk	301:310	human milk	301:310	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	12	theme	free	267:270	arg1	oligosaccharides					239:254	Human milk oligosaccharides	228:254	Human milk oligosaccharides (HMOs)	228:261	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	12	theme	free	267:270	arg1	glycans					272:278	free glycans	267:278	free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants	267:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	3	13	from	differences	651:661	arg1	composition					675:685	the HMOs composition	666:685	the HMOs composition	666:685	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	3	13	from	differences	651:661	arg1	concentrations					691:704	concentrations	691:704	concentrations	691:704	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	0	14	theme	ionization	196:205	arg1	spectrometry					214:225	electrospray ionization - mass spectrometry	183:225	electrospray ionization - mass spectrometry	183:225	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	4	15	theme	simple	770:775	arg1	extraction					782:791	a simple HMOs extraction	768:791	a simple HMOs extraction	768:791	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	0	16	theme	acidic	102:107	arg1	oligosaccharides					120:135	16 neutral and acidic human milk oligosaccharides	87:135	16 neutral and acidic human milk oligosaccharides	87:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	6	17	theme	Lewis	1258:1262	arg1	phenotypes					1264:1273	different Secretor and Lewis phenotypes	1235:1273	phenotypes	1264:1273	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	0	18	theme	mass	209:212	arg1	spectrometry					214:225	electrospray ionization - mass spectrometry	183:225	electrospray ionization - mass spectrometry	183:225	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	5	19	theme	human	1030:1034	arg1	milk					1036:1039	the human milk	1026:1039	the human milk obtained from 10 women	1026:1062	This method was validated and applied to analyze HMOs in the human milk obtained from 10 women.
30372996	6	20	theme	HMOs	1124:1127	arg1	quantification					1106:1119	accurate and reliable quantification	1084:1119	accurate and reliable quantification of HMOs	1084:1127	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	3	21	theme	HMOs	732:735	arg1	research					737:744	HMOs research	732:744	HMOs research	732:744	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	4	22	theme	HMOs	877:880	arg1	quantification					840:853	the simultaneous and absolute quantification	810:853	the simultaneous and absolute quantification of neutral and acidic HMOs	810:880	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	2	23	theme	phenotypes	577:586	arg1	influence					541:549	the direct influence	530:549	the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis	530:607	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	2	24	theme	HMOs	435:438	arg1	composition					440:450	The HMOs composition	431:450	The HMOs composition	431:450	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	6	25	with	women	1224:1228	arg1	Secretor					1245:1252	different Secretor and Lewis phenotypes	1235:1273	Secretor	1245:1252	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	6	25	with	women	1224:1228	arg1	phenotypes					1264:1273	different Secretor and Lewis phenotypes	1235:1273	phenotypes	1264:1273	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	1	26	from	present	290:296	arg1	milk					307:310	human milk	301:310	human milk	301:310	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	27	theme	present	290:296	arg1	oligosaccharides					239:254	Human milk oligosaccharides	228:254	Human milk oligosaccharides (HMOs)	228:261	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	27	theme	present	290:296	arg1	glycans					272:278	free glycans	267:278	free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants	267:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	0	28	theme	milk	115:118	arg1	oligosaccharides					120:135	16 neutral and acidic human milk oligosaccharides	87:135	16 neutral and acidic human milk oligosaccharides	87:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	6	29	theme	reliable	1097:1104	arg1	quantification					1106:1119	accurate and reliable quantification	1084:1119	accurate and reliable quantification of HMOs	1084:1127	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	6	30	theme	different	1235:1243	arg1	Secretor					1245:1252	different Secretor and Lewis phenotypes	1235:1273	Secretor	1245:1252	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	0	31	theme	human	109:113	arg1	oligosaccharides					120:135	16 neutral and acidic human milk oligosaccharides	87:135	16 neutral and acidic human milk oligosaccharides	87:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	1	32	theme	immune	375:380	arg1	system					382:387	the immune system	371:387	the immune system	371:387	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	0	33	theme	method	32:37	arg1	application					15:25	application	15:25	application	15:25	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	0	33	theme	method	32:37	arg1	Validation					0:9	Validation	0:9	Validation	0:9	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	1	34	theme	human	301:305	arg1	milk					307:310	human milk	301:310	human milk	301:310	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	4	35	theme	absolute	831:838	arg1	quantification					840:853	the simultaneous and absolute quantification	810:853	the simultaneous and absolute quantification of neutral and acidic HMOs	810:880	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	6	36	theme	accurate	1084:1091	arg1	quantification					1106:1119	accurate and reliable quantification	1084:1119	accurate and reliable quantification of HMOs	1084:1127	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	0	37	theme	oligosaccharides	120:135	arg1	quantification					69:82	the simultaneous absolute quantification	43:82	the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides	43:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	3	38	theme	Analytical	610:619	arg1	tool					724:727	a fundamental tool	710:727	a fundamental tool in HMOs research	710:744	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	3	38	theme	Analytical	610:619	arg1	methods					621:627	Analytical methods	610:627	Analytical methods that can identify the differences in the HMOs composition and concentrations	610:704	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	6	39	from	differences	1158:1168	arg1	concentrations					1178:1191	HMOs concentrations	1173:1191	HMOs concentrations throughout lactation	1173:1212	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	1	40	theme	Human	228:232	arg1	HMOs					257:260	HMOs	257:260	HMOs	257:260	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	40	theme	Human	228:232	arg1	oligosaccharides					239:254	Human milk oligosaccharides	228:254	Human milk oligosaccharides (HMOs)	228:261	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	40	theme	Human	228:232	arg1	glycans					272:278	free glycans	267:278	free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants	267:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	2	41	theme	direct	534:539	arg1	influence					541:549	the direct influence	530:549	the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis	530:607	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	0	42	theme	carbon	152:157	arg1	chromatography					166:179	graphitized carbon liquid chromatography	140:179	graphitized carbon liquid chromatography	140:179	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	4	43	theme	acidic	870:875	arg1	HMOs					877:880	neutral and acidic HMOs	858:880	neutral and acidic HMOs	858:880	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	6	44	theme	HMOs	1173:1176	arg1	concentrations					1178:1191	HMOs concentrations	1173:1191	HMOs concentrations throughout lactation	1173:1212	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	3	45	from	tool	724:727	arg1	research					737:744	HMOs research	732:744	HMOs research	732:744	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	0	46	theme	graphitized	140:150	arg1	chromatography					166:179	graphitized carbon liquid chromatography	140:179	graphitized carbon liquid chromatography	140:179	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	1	47	theme	brain	401:405	arg1	development					407:417	brain development	401:417	brain development in infants	401:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	4	48	theme	neutral	858:864	arg1	HMOs					877:880	neutral and acidic HMOs	858:880	neutral and acidic HMOs	858:880	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	6	49	used	used	1140:1143	arg2	method					1070:1075	This method	1065:1075	This method	1065:1075	This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
30372996	4	50	theme	liquid	904:909	arg1	spectrometry					955:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	2	51	theme	Lewis	571:575	arg1	phenotypes					577:586	the Secretor and Lewis phenotypes	554:586	phenotypes	577:586	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	1	52	theme	milk	234:237	arg1	HMOs					257:260	HMOs	257:260	HMOs	257:260	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	52	theme	milk	234:237	arg1	oligosaccharides					239:254	Human milk oligosaccharides	228:254	Human milk oligosaccharides (HMOs)	228:261	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	1	52	theme	milk	234:237	arg1	glycans					272:278	free glycans	267:278	free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants	267:428	Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants.
30372996	0	53	theme	liquid	159:164	arg1	chromatography					166:179	graphitized carbon liquid chromatography	140:179	graphitized carbon liquid chromatography	140:179	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	3	54	theme	HMOs	670:673	arg1	composition					675:685	the HMOs composition	666:685	the HMOs composition	666:685	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	4	55	theme	HMOs	777:780	arg1	extraction					782:791	a simple HMOs extraction	768:791	a simple HMOs extraction	768:791	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	4	56	theme	carbon	897:902	arg1	spectrometry					955:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	2	57	from	influence	541:549	arg1	biosynthesis					596:607	HMOs biosynthesis	591:607	HMOs biosynthesis	591:607	The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis.
30372996	0	58	theme	absolute	60:67	arg1	quantification					69:82	the simultaneous absolute quantification	43:82	the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides	43:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	4	59	theme	simultaneous	814:825	arg1	quantification					840:853	the simultaneous and absolute quantification	810:853	the simultaneous and absolute quantification of neutral and acidic HMOs	810:880	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	4	60	theme	chromatography-electrospray	911:937	arg1	spectrometry					955:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry	885:966	This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry.
30372996	0	61	theme	simultaneous	47:58	arg1	quantification					69:82	the simultaneous absolute quantification	43:82	the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides	43:135	Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry.
30372996	3	62	theme	fundamental	712:722	arg1	tool					724:727	a fundamental tool	710:727	a fundamental tool in HMOs research	710:744	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
30372996	3	62	theme	fundamental	712:722	arg1	methods					621:627	Analytical methods	610:627	Analytical methods that can identify the differences in the HMOs composition and concentrations	610:704	Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research.
31826529	9	0	theme	cells	1169:1173	arg1	proliferation					1128:1140	the proliferation	1124:1140	the proliferation of normal human fibroblast cells	1124:1173	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	1	1	theme	bacterial	147:155	arg1	BC					168:169	BC	168:169	BC	168:169	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	1	1	theme	bacterial	147:155	arg1	cellulose					157:165	bacterial cellulose	147:165	bacterial cellulose (BC)	147:170	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	5	2	theme	SPG	760:762	arg1	entrance					748:755	the successful entrance	733:755	the successful entrance of SPG into BC fibrils	733:778	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	6	3	theme	±	898:898	arg1	BC					889:890	neat BC	884:890	neat BC (4.41 ± 0.38 MPa)	884:908	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	6	3	theme	±	898:898	arg1	0.38 MPa					900:907	4.41 ± 0.38 MPa	893:907	4.41 ± 0.38 MPa	893:907	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	1	4	theme	cellulose	157:165	arg1	biopolymers					196:206	bacterial cellulose (BC) and schizophyllan (SPG) biopolymers	147:206	bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties	147:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	1	5	theme	swelling	234:241	arg1	properties					261:270	improved mechanical, swelling and antibacterial properties	213:270	improved mechanical, swelling and antibacterial properties	213:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	5	6	theme	grafted	709:715	arg1	BC/SPG					717:722	amine grafted BC/SPG	703:722	amine grafted BC/SPG	703:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	3	7	from	peak	415:418	arg1	pattern					439:445	the XRD pattern	431:445	the XRD pattern of amine-grafted BC/SPG membrane	431:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	5	8	theme	distinct	650:657	arg1	stages					659:664	Two distinct stages	646:664	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG	646:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	4	9	theme	fibrillar	582:590	arg1	network					592:598	the fibrillar network	578:598	the fibrillar network of BC	578:604	SEM images of amine-grafted BC/SPG showed that the fibrillar network of BC was totally covered with schizophyllan.
31826529	5	10	theme	BC/SPG	717:722	arg1	thermo-gram					688:698	TGA thermo-gram	684:698	TGA thermo-gram of amine grafted BC/SPG	684:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	1	11	theme	antibacterial	247:259	arg1	properties					261:270	improved mechanical, swelling and antibacterial properties	213:270	improved mechanical, swelling and antibacterial properties	213:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	6	12	theme	Tensile	781:787	arg1	strength					789:796	Tensile strength	781:796	Tensile strength of the amine-grafted BC/SPG	781:824	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	3	13	theme	BC	519:520	arg1	network					522:528	BC network	519:528	BC network	519:528	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	5	14	from	thermo-gram	688:698	arg1	stages					659:664	Two distinct stages	646:664	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG	646:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	6	15	theme	neat	884:887	arg1	BC					889:890	neat BC	884:890	neat BC (4.41 ± 0.38 MPa)	884:908	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	6	15	theme	neat	884:887	arg1	0.38 MPa					900:907	4.41 ± 0.38 MPa	893:907	4.41 ± 0.38 MPa	893:907	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	7	16	theme	highest	969:975	arg1	degree					986:991	the highest swelling degree	965:991	the highest swelling degree (800 ± 80%)	965:1003	It also showed the highest swelling degree (800 ± 80%).
31826529	7	16	theme	highest	969:975	arg1	%					1002:1002	800 ± 80%	994:1002	800 ± 80%	994:1002	It also showed the highest swelling degree (800 ± 80%).
31826529	9	17	theme	MTT	1070:1072	arg1	assay					1074:1078	MTT assay	1070:1078	MTT assay	1070:1078	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	6	18	theme	amine-grafted	914:926	arg1	BC					928:929	amine-grafted BC	914:929	amine-grafted BC (7.90 ± 0.71 MPa)	914:947	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	6	18	theme	amine-grafted	914:926	arg1	7.90 ± 0.71 MPa					932:946	7.90 ± 0.71 MPa	932:946	7.90 ± 0.71 MPa	932:946	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	0	19	theme	novel	2:6	arg1	hydrogel					37:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel based on aminated bacterial cellulose and schizophyllan.
31826529	8	20	theme	antibacterial	1046:1058	arg1	activity					1060:1067	moderate antibacterial activity	1037:1067	moderate antibacterial activity	1037:1067	Amine-grafted BC/SPG exhibited moderate antibacterial activity.
31826529	5	21	theme	weight	669:674	arg1	loss					676:679	weight loss	669:679	weight loss in TGA thermo-gram of amine grafted BC/SPG	669:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	8	22	theme	moderate	1037:1044	arg1	activity					1060:1067	moderate antibacterial activity	1037:1067	moderate antibacterial activity	1037:1067	Amine-grafted BC/SPG exhibited moderate antibacterial activity.
31826529	2	23	theme	better	372:377	arg1	interaction					379:389	better interaction	372:389	better interaction with SPG	372:398	BC was firstly functionalized using 3-aminopropyl triethoxysilane (APTES) to provide better interaction with SPG.
31826529	0	24	theme	antibacterial	23:35	arg1	hydrogel					37:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel based on aminated bacterial cellulose and schizophyllan.
31826529	5	25	theme	TGA	684:686	arg1	thermo-gram					688:698	TGA thermo-gram	684:698	TGA thermo-gram of amine grafted BC/SPG	684:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	1	26	theme	schizophyllan	176:188	arg1	biopolymers					196:206	bacterial cellulose (BC) and schizophyllan (SPG) biopolymers	147:206	bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties	147:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	0	27	theme	double-network	8:21	arg1	hydrogel					37:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel	0:44	A novel double-network antibacterial hydrogel based on aminated bacterial cellulose and schizophyllan.
31826529	4	28	theme	BC/SPG	559:564	arg1	images					535:540	SEM images	531:540	SEM images of amine-grafted BC/SPG	531:564	SEM images of amine-grafted BC/SPG showed that the fibrillar network of BC was totally covered with schizophyllan.
31826529	9	29	theme	amine-grafted	1092:1104	arg1	BC/SPG					1106:1111	amine-grafted BC/SPG	1092:1111	amine-grafted BC/SPG	1092:1111	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	3	30	theme	amine-grafted	450:462	arg1	membrane					471:478	amine-grafted BC/SPG membrane	450:478	amine-grafted BC/SPG membrane	450:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	3	31	theme	diffraction	403:413	arg1	peak					415:418	A diffraction peak	401:418	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane	401:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	4	32	theme	amine-grafted	545:557	arg1	BC/SPG					559:564	amine-grafted BC/SPG	545:564	amine-grafted BC/SPG	545:564	SEM images of amine-grafted BC/SPG showed that the fibrillar network of BC was totally covered with schizophyllan.
31826529	3	33	theme	XRD	435:437	arg1	pattern					439:445	the XRD pattern	431:445	the XRD pattern of amine-grafted BC/SPG membrane	431:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	5	34	theme	BC	769:770	arg1	fibrils					772:778	BC fibrils	769:778	BC fibrils	769:778	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	5	35	theme	amine	703:707	arg1	BC/SPG					717:722	amine grafted BC/SPG	703:722	amine grafted BC/SPG	703:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	9	36	theme	human	1152:1156	arg1	cells					1169:1173	normal human fibroblast cells	1145:1173	normal human fibroblast cells	1145:1173	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	0	37	theme	aminated	55:62	arg1	cellulose					74:82	aminated bacterial cellulose	55:82	aminated bacterial cellulose	55:82	A novel double-network antibacterial hydrogel based on aminated bacterial cellulose and schizophyllan.
31826529	5	38	from	stages	659:664	arg1	thermo-gram					688:698	TGA thermo-gram	684:698	TGA thermo-gram of amine grafted BC/SPG	684:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	4	39	theme	SEM	531:533	arg1	images					535:540	SEM images	531:540	SEM images of amine-grafted BC/SPG	531:564	SEM images of amine-grafted BC/SPG showed that the fibrillar network of BC was totally covered with schizophyllan.
31826529	3	40	theme	BC/SPG	464:469	arg1	membrane					471:478	amine-grafted BC/SPG membrane	450:478	amine-grafted BC/SPG membrane	450:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	7	41	theme	swelling	977:984	arg1	degree					986:991	the highest swelling degree	965:991	the highest swelling degree (800 ± 80%)	965:1003	It also showed the highest swelling degree (800 ± 80%).
31826529	7	41	theme	swelling	977:984	arg1	%					1002:1002	800 ± 80%	994:1002	800 ± 80%	994:1002	It also showed the highest swelling degree (800 ± 80%).
31826529	8	42	theme	Amine-grafted	1006:1018	arg1	BC/SPG					1020:1025	Amine-grafted BC/SPG	1006:1025	Amine-grafted BC/SPG	1006:1025	Amine-grafted BC/SPG exhibited moderate antibacterial activity.
31826529	5	43	theme	loss	676:679	arg1	stages					659:664	Two distinct stages	646:664	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG	646:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	1	44	theme	novel	120:124	arg1	hydrogel					126:133	a novel hydrogel	118:133	a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties	118:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	9	45	theme	fibroblast	1158:1167	arg1	cells					1169:1173	normal human fibroblast cells	1145:1173	normal human fibroblast cells	1145:1173	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	5	46	from	loss	676:679	arg1	thermo-gram					688:698	TGA thermo-gram	684:698	TGA thermo-gram of amine grafted BC/SPG	684:722	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	6	47	theme	BC/SPG	819:824	arg1	strength					789:796	Tensile strength	781:796	Tensile strength of the amine-grafted BC/SPG	781:824	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	9	48	theme	normal	1145:1150	arg1	cells					1169:1173	normal human fibroblast cells	1145:1173	normal human fibroblast cells	1145:1173	MTT assay showed that amine-grafted BC/SPG stimulated the proliferation of normal human fibroblast cells.
31826529	3	49	theme	membrane	471:478	arg1	pattern					439:445	the XRD pattern	431:445	the XRD pattern of amine-grafted BC/SPG membrane	431:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	2	50	theme	3-aminopropyl	323:335	arg1	triethoxysilane					337:351	3-aminopropyl triethoxysilane	323:351	3-aminopropyl triethoxysilane (APTES)	323:359	BC was firstly functionalized using 3-aminopropyl triethoxysilane (APTES) to provide better interaction with SPG.
31826529	2	50	theme	3-aminopropyl	323:335	arg1	APTES					354:358	APTES	354:358	APTES	354:358	BC was firstly functionalized using 3-aminopropyl triethoxysilane (APTES) to provide better interaction with SPG.
31826529	5	51	theme	successful	737:746	arg1	entrance					748:755	the successful entrance	733:755	the successful entrance of SPG into BC fibrils	733:778	Two distinct stages of weight loss in TGA thermo-gram of amine grafted BC/SPG verified the successful entrance of SPG into BC fibrils.
31826529	6	52	theme	amine-grafted	805:817	arg1	BC/SPG					819:824	the amine-grafted BC/SPG	801:824	the amine-grafted BC/SPG	801:824	Tensile strength of the amine-grafted BC/SPG considerably increased to 42.19 ± 7.16 MPa as compared to neat BC (4.41 ± 0.38 MPa) and amine-grafted BC (7.90 ± 0.71 MPa).
31826529	3	53	from	5.7°	423:426	arg1	peak					415:418	A diffraction peak	401:418	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane	401:478	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	3	54	theme	SPG	510:512	arg1	intercalation					493:505	the intercalation	489:505	the intercalation of SPG into BC network	489:528	A diffraction peak at 5.7° in the XRD pattern of amine-grafted BC/SPG membrane revealed the intercalation of SPG into BC network.
31826529	1	55	theme	improved	213:220	arg1	properties					261:270	improved mechanical, swelling and antibacterial properties	213:270	improved mechanical, swelling and antibacterial properties	213:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	0	56	theme	bacterial	64:72	arg1	cellulose					74:82	aminated bacterial cellulose	55:82	aminated bacterial cellulose	55:82	A novel double-network antibacterial hydrogel based on aminated bacterial cellulose and schizophyllan.
31826529	2	57	with	interaction	379:389	arg1	SPG					396:398	SPG	396:398	SPG	396:398	BC was firstly functionalized using 3-aminopropyl triethoxysilane (APTES) to provide better interaction with SPG.
31826529	4	58	theme	BC	603:604	arg1	network					592:598	the fibrillar network	578:598	the fibrillar network of BC	578:604	SEM images of amine-grafted BC/SPG showed that the fibrillar network of BC was totally covered with schizophyllan.
31826529	1	59	with	biopolymers	196:206	arg1	properties					261:270	improved mechanical, swelling and antibacterial properties	213:270	improved mechanical, swelling and antibacterial properties	213:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31826529	1	60	theme	mechanical	222:231	arg1	properties					261:270	improved mechanical, swelling and antibacterial properties	213:270	improved mechanical, swelling and antibacterial properties	213:270	In this study, a novel hydrogel composed of bacterial cellulose (BC) and schizophyllan (SPG) biopolymers with improved mechanical, swelling and antibacterial properties was developed.
31539750	4	0	theme	scaffold	806:813	arg1	production					790:799	the production	786:799	the production of a scaffold that simulates the structure of the native peripheral nerve	786:873	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	5	1	dep	in	876:877	arg1	vitro					879:883	vitro	879:883	vitro	879:883	in vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.
31539750	5	2	theme	hydrogels	946:954	arg1	biocompatibility					909:924	biocompatibility	909:924	biocompatibility of the manufactured hydrogels	909:954	in vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.
31539750	4	3	theme	native	851:856	arg1	nerve					869:873	the native peripheral nerve	847:873	the native peripheral nerve	847:873	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	1	4	theme	new	128:130	arg1	method					132:137	a new method	126:137	a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions	126:253	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	1	5	theme	vinyl	227:231	arg1	alcohol					233:239	vinyl alcohol	227:239	vinyl alcohol	227:239	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	3	6	theme	chitosan	631:638	arg1	compositions					640:651	the chitosan compositions	627:651	the chitosan compositions	627:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	7	theme	hydrogels	509:517	arg1	Structure					492:500	Structure	492:500	Structure of the hydrogels	492:517	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	4	8	theme	thawing	685:691	arg1	cycle					663:667	A single cycle	654:667	A single cycle of freezing and thawing of the produced hydrogels	654:717	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	0	9	theme	directed	78:85	arg1	growth					87:92	directed growth	78:92	directed growth of animal cells	78:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	0	10	theme	animal	97:102	arg1	cells					104:108	animal cells	97:108	animal cells	97:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	4	11	theme	single	656:661	arg1	cycle					663:667	A single cycle	654:667	A single cycle of freezing and thawing of the produced hydrogels	654:717	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	4	12	theme	hydrogels	709:717	arg1	thawing					685:691	thawing	685:691	thawing	685:691	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	4	12	theme	hydrogels	709:717	arg1	freezing					672:679	freezing	672:679	freezing	672:679	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	2	13	theme	material	425:432	arg1	structure					408:416	structure	408:416	structure of the material	408:432	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	5	14	theme	in	876:877	arg1	assays					895:900	in vitro cytotoxic assays	876:900	in vitro cytotoxic assays	876:900	in vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.
31539750	2	15	theme	optimal	363:369	arg1	composition					371:381	the optimal composition	359:381	the optimal composition	359:381	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	0	16	theme	Multichannel	0:11	arg1	hydrogel					13:20	Multichannel hydrogel	0:20	Multichannel hydrogel	0:20	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	2	17	theme	biological	286:295	arg1	studies					297:303	Microscopic and biological studies	270:303	Microscopic and biological studies of the obtained hydrogels	270:329	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	4	18	theme	produced	700:707	arg1	hydrogels					709:717	the produced hydrogels	696:717	the produced hydrogels	696:717	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	2	19	from	epineurium	453:462	arg1	nerve					485:489	a nerve	483:489	a nerve	483:489	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	4	20	theme	peripheral	858:867	arg1	nerve					869:873	the native peripheral nerve	847:873	the native peripheral nerve	847:873	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	5	21	theme	cytotoxic	885:893	arg1	assays					895:900	in vitro cytotoxic assays	876:900	in vitro cytotoxic assays	876:900	in vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.
31539750	1	22	theme	hydrogels	157:165	arg1	production					143:152	production	143:152	production of hydrogels with oriented multichannel structure	143:202	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	4	23	theme	freezing	672:679	arg1	cycle					663:667	A single cycle	654:667	A single cycle of freezing and thawing of the produced hydrogels	654:717	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	0	24	theme	cells	104:108	arg1	growth					87:92	directed growth	78:92	directed growth of animal cells	78:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	2	25	theme	Microscopic	270:280	arg1	studies					297:303	Microscopic and biological studies	270:303	Microscopic and biological studies of the obtained hydrogels	270:329	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	3	26	from	concentration	560:572	arg1	compositions					640:651	the chitosan compositions	627:651	the chitosan compositions	627:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	0	27	theme	chitosan-poly	33:45	arg1	composition					62:72	a chitosan-poly(vinyl alcohol) composition	31:72	a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells	31:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	1	28	with	hydrogels	157:165	arg1	structure					194:202	oriented multichannel structure	172:202	oriented multichannel structure	172:202	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	1	29	dep	chitosan-poly	213:225	arg1	alcohol					233:239	vinyl alcohol	227:239	vinyl alcohol	227:239	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	3	30	theme	concentration	560:572	arg1	variation					535:543	variation	535:543	variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	535:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	1	31	theme	oriented	172:179	arg1	structure					194:202	oriented multichannel structure	172:202	oriented multichannel structure	172:202	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	4	32	theme	nerve	869:873	arg1	structure					834:842	the structure	830:842	the structure of the native peripheral nerve	830:873	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	1	33	theme	multichannel	181:192	arg1	structure					194:202	oriented multichannel structure	172:202	oriented multichannel structure	172:202	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31539750	0	34	theme	vinyl	47:51	arg1	composition					62:72	a chitosan-poly(vinyl alcohol) composition	31:72	a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells	31:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	2	35	from	perineurium	468:478	arg1	nerve					485:489	a nerve	483:489	a nerve	483:489	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	2	36	theme	hydrogels	321:329	arg1	studies					297:303	Microscopic and biological studies	270:303	Microscopic and biological studies of the obtained hydrogels	270:329	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	2	37	dep	epineurium	453:462	arg1	the					449:451	the	449:451	the	449:451	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	3	38	theme	vinyl	599:603	arg1	alcohol					605:611	vinyl alcohol	599:611	vinyl alcohol	599:611	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	38	theme	vinyl	599:603	arg1	poly					594:597	poly	594:597	poly(vinyl alcohol)	594:612	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	39	theme	initial	552:558	arg1	concentration					560:572	the initial concentration	548:572	the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	548:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	2	40	theme	obtained	312:319	arg1	hydrogels					321:329	the obtained hydrogels	308:329	the obtained hydrogels	308:329	Microscopic and biological studies of the obtained hydrogels were conducted to determine the optimal composition, which would ensure that structure of the material mimics that of the epineurium and perineurium in a nerve.
31539750	4	41	theme	structural	737:746	arg1	heterogeneity					748:760	lower structural heterogeneity	731:760	lower structural heterogeneity	731:760	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	5	42	theme	manufactured	933:944	arg1	hydrogels					946:954	the manufactured hydrogels	929:954	the manufactured hydrogels	929:954	in vitro cytotoxic assays showed biocompatibility of the manufactured hydrogels.
31539750	4	43	theme	lower	731:735	arg1	heterogeneity					748:760	lower structural heterogeneity	731:760	lower structural heterogeneity	731:760	A single cycle of freezing and thawing of the produced hydrogels resulted in lower structural heterogeneity, which is promising for the production of a scaffold that simulates the structure of the native peripheral nerve.
31539750	3	44	from	compositions	640:651	arg1	variation					535:543	variation	535:543	variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	535:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	45	theme	poly	594:597	arg1	concentration					560:572	the initial concentration	548:572	the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	548:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	46	from	variation	535:543	arg1	compositions					640:651	the chitosan compositions	627:651	the chitosan compositions	627:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	3	47	theme	acid	619:622	arg1	concentration					560:572	the initial concentration	548:572	the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	548:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	0	48	theme	alcohol	53:59	arg1	composition					62:72	a chitosan-poly(vinyl alcohol) composition	31:72	a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells	31:108	Multichannel hydrogel based on a chitosan-poly(vinyl alcohol) composition for directed growth of animal cells.
31539750	3	49	theme	precipitant	581:591	arg1	concentration					560:572	the initial concentration	548:572	the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions	548:651	Structure of the hydrogels was adjusted by variation of the initial concentration of the precipitant, poly(vinyl alcohol), and acid in the chitosan compositions.
31539750	1	50	theme	chitosan-poly	213:225	arg1	compositions					242:253	chitosan-poly(vinyl alcohol) compositions	213:253	chitosan-poly(vinyl alcohol) compositions	213:253	In this study, a new method for production of hydrogels with oriented multichannel structure based on chitosan-poly(vinyl alcohol) compositions was developed.
31235516	14	0	theme	polysaccharides	2399:2413	arg1	synthesis					2380:2388	the synthesis	2376:2388	the synthesis of spore polysaccharides	2376:2413	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	4	1	with	microscopy	654:663	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	6	2	theme	mutant	1163:1168	arg1	spores					1170:1175	yfnH-D mutant spores	1156:1175	yfnH-D mutant spores	1156:1175	In addition, yfnH-D mutant spores exhibit a crust with an unusual weblike morphology.
31235516	4	3	theme	India	544:548	arg1	staining					554:561	India ink staining	544:561	India ink staining	544:561	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	3	theme	India	544:548	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	4	theme	saccharide	1800:1809	arg1	pathways					1821:1828	multiple saccharide synthesis pathways	1791:1828	multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1791:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	5	5	from	mutations	904:912	arg1	operon					925:930	the sps operon	917:930	the sps operon	917:930	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	14	6	theme	responsible	2360:2370	arg1	enzymes					2352:2358	the enzymes	2348:2358	the enzymes responsible for the synthesis of spore polysaccharides	2348:2413	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	11	7	with	treatments	2034:2043	arg1	agents					2064:2069	antimicrobial agents	2050:2069	antimicrobial agents	2050:2069	The dormant spore state allows these species to survive even the harshest treatments with antimicrobial agents.
31235516	4	8	theme	red	618:620	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	14	9	theme	spore	2393:2397	arg1	polysaccharides					2399:2413	spore polysaccharides	2393:2413	spore polysaccharides	2393:2413	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	5	10	from	mutations	1070:1078	arg1	cluster					1103:1109	the yfnH-D cluster	1092:1109	the yfnH-D cluster	1092:1109	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	5	10	from	mutations	1070:1078	arg1	cgeD					1083:1086	cgeD	1083:1086	cgeD	1083:1086	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	6	11	theme	weblike	1209:1215	arg1	morphology					1217:1226	an unusual weblike morphology	1198:1226	an unusual weblike morphology	1198:1226	In addition, yfnH-D mutant spores exhibit a crust with an unusual weblike morphology.
31235516	1	12	theme	dormant	203:209	arg1	spores					223:228	spores	223:228	spores	223:228	Polysaccharides (PS) decorate the surface of dormant endospores (spores).
31235516	1	12	theme	dormant	203:209	arg1	endospores					211:220	dormant endospores	203:220	dormant endospores (spores)	203:229	Polysaccharides (PS) decorate the surface of dormant endospores (spores).
31235516	4	13	theme	outermost	855:863	arg1	layer					871:875	the outermost spore layer	851:875	the outermost spore layer	851:875	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	13	theme	outermost	855:863	arg1	morphology					811:820	the morphology	807:820	the morphology	807:820	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	7	14	theme	mutant	1264:1269	arg1	spores					1271:1276	sps mutant spores	1260:1276	sps mutant spores	1260:1276	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	10	15	theme	Many	1878:1881	arg1	bacteria					1883:1890	Many bacteria	1878:1890	Many bacteria	1878:1890	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	0	16	theme	Modification	136:147	arg1	Enzymes					149:155	Glucose Modification Enzymes	128:155	Glucose Modification Enzymes	128:155	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	15	17	theme	polysaccharides	2659:2673	arg1	allocation					2637:2646	allocation	2637:2646	allocation	2637:2646	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	15	17	theme	polysaccharides	2659:2673	arg1	assembly					2624:2631	assembly	2624:2631	assembly	2624:2631	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	11	18	theme	spore	1972:1976	arg1	state					1978:1982	The dormant spore state	1960:1982	The dormant spore state	1960:1982	The dormant spore state allows these species to survive even the harshest treatments with antimicrobial agents.
31235516	10	19	theme	surface	1848:1854	arg1	properties.IMPORTANCE					1856:1876	spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1842:1957	spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1842:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	14	20	theme	specific	2515:2522	arg1	carbohydrates					2524:2536	specific carbohydrates	2515:2536	specific carbohydrates	2515:2536	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	9	21	theme	gene	1600:1603	arg1	cluster					1605:1611	each gene cluster	1595:1611	each gene cluster	1595:1611	Our data are consistent with the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose.
31235516	7	22	theme	hydrophobic	1233:1243	arg1	phenotype					1245:1253	The hydrophobic phenotype	1229:1253	The hydrophobic phenotype from sps mutant spores	1229:1276	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	5	23	theme	spores	960:965	arg1	production					946:955	the production	942:955	the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition	942:1061	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	16	24	theme	spore-forming	2743:2755	arg1	bacteria					2757:2764	infectious spore-forming bacteria	2732:2764	infectious spore-forming bacteria	2732:2764	Similar mechanisms could be modulating the dispersal of infectious spore-forming bacteria.
31235516	4	25	theme	scanning	636:643	arg1	microscopy					654:663	scanning electron microscopy	636:663	scanning electron microscopy (SEM)	636:669	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	25	theme	scanning	636:643	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	25	theme	scanning	636:643	arg1	SEM					666:668	SEM	666:668	SEM	666:668	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	12	26	from	natural	2187:2193	arg1	habitats					2173:2180	various habitats	2165:2180	various habitats from natural to human-made environments	2165:2220	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	0	27	from	Expansion	0:8	arg1	subtilis					64:71	Bacillus subtilis	55:71	Bacillus subtilis	55:71	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	4	28	from	contributions	693:705	arg1	layer					871:875	the outermost spore layer	851:875	the outermost spore layer	851:875	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	28	from	contributions	693:705	arg1	properties					826:835	properties	826:835	properties	826:835	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	28	from	contributions	693:705	arg1	morphology					811:820	the morphology	807:820	the morphology	807:820	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	15	29	theme	gene	2586:2589	arg1	clusters					2591:2598	several sporulation gene clusters	2566:2598	several sporulation gene clusters	2566:2598	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	5	30	theme	visible	1004:1010	arg1	crust					1012:1016	a visible crust	1002:1016	a visible crust	1002:1016	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	11	31	theme	antimicrobial	2050:2062	arg1	agents					2064:2069	antimicrobial agents	2050:2069	antimicrobial agents	2050:2069	The dormant spore state allows these species to survive even the harshest treatments with antimicrobial agents.
31235516	3	32	theme	synthesis	376:384	arg1	enzymes					386:392	PS synthesis enzymes	373:392	PS synthesis enzymes produced during the late stages of sporulation	373:439	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	2	33	theme	model	239:243	arg1	organism					245:252	the model organism	235:252	the model organism for sporulation	235:268	In the model organism for sporulation, Bacillus subtilis, the composition of the spore PS is not known in detail.
31235516	12	34	theme	Spore	2072:2076	arg1	properties					2086:2095	Spore surface properties	2072:2095	Spore surface properties	2072:2095	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	9	35	with	consistent	1558:1567	arg1	possibility					1578:1588	the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose	1574:1679	the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose	1574:1679	Our data are consistent with the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose.
31235516	0	36	theme	Surface	23:29	arg1	Layer					46:50	the Spore Surface Polysaccharide Layer	13:50	the Spore Surface Polysaccharide Layer	13:50	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	5	37	theme	reduced	1041:1047	arg1	deposition					1052:1061	reduced PS deposition	1041:1061	reduced PS deposition	1041:1061	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	15	38	theme	surface	2651:2657	arg1	polysaccharides					2659:2673	surface polysaccharides	2651:2673	surface polysaccharides	2651:2673	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	14	39	theme	surface	2440:2446	arg1	properties					2448:2457	spore surface properties	2434:2457	spore surface properties such as hydrophobicity	2434:2480	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	14	39	theme	surface	2440:2446	arg1	hydrophobicity					2467:2480	hydrophobicity	2467:2480	hydrophobicity	2467:2480	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	16	40	theme	Similar	2676:2682	arg1	mechanisms					2684:2693	Similar mechanisms	2676:2693	Similar mechanisms	2676:2693	Similar mechanisms could be modulating the dispersal of infectious spore-forming bacteria.
31235516	8	41	theme	glucose-1-phosphate	1427:1445	arg1	nucleotidyltransferases					1447:1469	glucose-1-phosphate nucleotidyltransferases	1427:1469	glucose-1-phosphate nucleotidyltransferases	1427:1469	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	13	42	from	surface	2233:2239	arg1	crust					2263:2267	crust	2263:2267	crust	2263:2267	The spore surface in Bacillus subtilis (crust) is composed of a combination of proteins and polysaccharides.
31235516	13	42	from	surface	2233:2239	arg1	subtilis					2253:2260	Bacillus subtilis	2244:2260	Bacillus subtilis (crust)	2244:2268	The spore surface in Bacillus subtilis (crust) is composed of a combination of proteins and polysaccharides.
31235516	3	43	theme	spore	448:452	arg1	properties					462:471	spore surface properties	448:471	spore surface properties	448:471	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	0	44	theme	Layer	46:50	arg1	Expansion					0:8	Expansion	0:8	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes	0:155	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	4	45	theme	bacterial	494:502	arg1	adhesion					504:511	bacterial adhesion	494:511	bacterial adhesion to hydrocarbons (BATH) assays	494:541	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	45	theme	bacterial	494:502	arg1	BATH					530:533	BATH	530:533	BATH	530:533	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	46	theme	gene	727:730	arg1	clusters					732:739	four sporulation gene clusters	710:739	four sporulation gene clusters	710:739	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	46	theme	gene	727:730	arg1	ytdA-ytcABC					773:783	ytdA-ytcABC	773:783	ytdA-ytcABC	773:783	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	46	theme	gene	727:730	arg1	cgeAB-cgeCDE					790:801	cgeAB-cgeCDE	790:801	cgeAB-cgeCDE	790:801	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	46	theme	gene	727:730	arg1	yfnHGF-yfnED					759:770	yfnHGF-yfnED	759:770	yfnHGF-yfnED	759:770	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	46	theme	gene	727:730	arg1	spsABCDEFGHIJKL					742:756	spsABCDEFGHIJKL	742:756	spsABCDEFGHIJKL	742:756	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	2	47	theme	spore	313:317	arg1	PS					319:320	the spore PS	309:320	the spore PS	309:320	In the model organism for sporulation, Bacillus subtilis, the composition of the spore PS is not known in detail.
31235516	10	48	theme	resistant	1942:1950	arg1	spores					1952:1957	highly resistant spores	1935:1957	highly resistant spores	1935:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	4	49	theme	ink	550:552	arg1	staining					554:561	India ink staining	544:561	India ink staining	544:561	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	49	theme	ink	550:552	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	50	theme	PS	1727:1728	arg1	layer					1730:1734	the PS layer	1723:1734	the PS layer	1723:1734	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	8	51	theme	spore	1535:1539	arg1	PS					1541:1542	the spore PS	1531:1542	the spore PS	1531:1542	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	5	52	theme	sps	921:923	arg1	operon					925:930	the sps operon	917:930	the sps operon	917:930	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	7	53	theme	yfnHGF	1350:1355	arg1	operon					1357:1362	the yfnHGF operon	1346:1362	the yfnHGF operon	1346:1362	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	10	54	theme	surface	1749:1755	arg1	hydrophobicity					1757:1770	spore surface hydrophobicity	1743:1770	spore surface hydrophobicity	1743:1770	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	6	55	theme	yfnH-D	1156:1161	arg1	spores					1170:1175	yfnH-D mutant spores	1156:1175	yfnH-D mutant spores	1156:1175	In addition, yfnH-D mutant spores exhibit a crust with an unusual weblike morphology.
31235516	5	56	theme	yfnH-D	1096:1101	arg1	cluster					1103:1109	the yfnH-D cluster	1092:1109	the yfnH-D cluster	1092:1109	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	16	57	theme	bacteria	2757:2764	arg1	dispersal					2719:2727	the dispersal	2715:2727	the dispersal of infectious spore-forming bacteria	2715:2764	Similar mechanisms could be modulating the dispersal of infectious spore-forming bacteria.
31235516	8	58	theme	distinct	1512:1519	arg1	manner					1521:1526	a distinct manner	1510:1526	a distinct manner	1510:1526	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	10	59	theme	multiple	1791:1798	arg1	pathways					1821:1828	multiple saccharide synthesis pathways	1791:1828	multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1791:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	15	60	theme	several	2566:2572	arg1	clusters					2591:2598	several sporulation gene clusters	2566:2598	several sporulation gene clusters	2566:2598	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	11	61	theme	harshest	2025:2032	arg1	treatments					2034:2043	the harshest treatments	2021:2043	the harshest treatments with antimicrobial agents	2021:2069	The dormant spore state allows these species to survive even the harshest treatments with antimicrobial agents.
31235516	7	62	theme	second	1305:1310	arg1	mutation					1312:1319	a second mutation	1303:1319	a second mutation inactivating any gene in the yfnHGF operon	1303:1362	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	7	63	from	gene	1338:1341	arg1	operon					1357:1362	the yfnHGF operon	1346:1362	the yfnHGF operon	1346:1362	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	4	64	theme	ruthenium	608:616	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	0	65	theme	Glucose	128:134	arg1	Enzymes					149:155	Glucose Modification Enzymes	128:155	Glucose Modification Enzymes	128:155	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	4	66	theme	crust	844:848	arg1	layer					871:875	the outermost spore layer	851:875	the outermost spore layer	851:875	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	66	theme	crust	844:848	arg1	properties					826:835	properties	826:835	properties	826:835	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	66	theme	crust	844:848	arg1	morphology					811:820	the morphology	807:820	the morphology	807:820	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	67	theme	electron	577:584	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	67	theme	electron	577:584	arg1	TEM					598:600	TEM	598:600	TEM	598:600	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	67	theme	electron	577:584	arg1	microscopy					586:595	transmission electron microscopy	564:595	transmission electron microscopy (TEM) with ruthenium red staining	564:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	68	theme	synthesis	1811:1819	arg1	pathways					1821:1828	multiple saccharide synthesis pathways	1791:1828	multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1791:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	6	69	theme	unusual	1201:1207	arg1	morphology					1217:1226	an unusual weblike morphology	1198:1226	an unusual weblike morphology	1198:1226	In addition, yfnH-D mutant spores exhibit a crust with an unusual weblike morphology.
31235516	6	70	with	crust	1187:1191	arg1	morphology					1217:1226	an unusual weblike morphology	1198:1226	an unusual weblike morphology	1198:1226	In addition, yfnH-D mutant spores exhibit a crust with an unusual weblike morphology.
31235516	1	71	theme	endospores	211:220	arg1	surface					192:198	the surface	188:198	the surface of dormant endospores (spores)	188:229	Polysaccharides (PS) decorate the surface of dormant endospores (spores).
31235516	4	72	with	staining	554:561	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	73	dep	properties.IMPORTANCE	1856:1876	arg1	characterized					1896:1908	characterized	1896:1908	are characterized by their ability to form highly resistant spores	1892:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	14	74	theme	carbohydrates	2524:2536	arg1	addition					2503:2510	the addition	2499:2510	the addition of specific carbohydrates	2499:2536	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	7	75	theme	sps	1260:1262	arg1	spores					1271:1276	sps mutant spores	1260:1276	sps mutant spores	1260:1276	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	4	76	theme	electron	645:652	arg1	microscopy					654:663	scanning electron microscopy	636:663	scanning electron microscopy (SEM)	636:669	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	76	theme	electron	645:652	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	76	theme	electron	645:652	arg1	SEM					666:668	SEM	666:668	SEM	666:668	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	77	theme	spore	1842:1846	arg1	properties.IMPORTANCE					1856:1876	spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1842:1957	spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores	1842:1957	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	11	78	theme	dormant	1964:1970	arg1	state					1978:1982	The dormant spore state	1960:1982	The dormant spore state	1960:1982	The dormant spore state allows these species to survive even the harshest treatments with antimicrobial agents.
31235516	3	79	theme	sporulation	429:439	arg1	stages					419:424	the late stages	410:424	the late stages of sporulation	410:439	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	2	80	theme	PS	319:320	arg1	composition					294:304	the composition	290:304	the composition of the spore PS	290:320	In the model organism for sporulation, Bacillus subtilis, the composition of the spore PS is not known in detail.
31235516	4	81	with	assays	536:541	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	16	82	theme	infectious	2732:2741	arg1	bacteria					2757:2764	infectious spore-forming bacteria	2732:2764	infectious spore-forming bacteria	2732:2764	Similar mechanisms could be modulating the dispersal of infectious spore-forming bacteria.
31235516	0	83	theme	Genes	88:92	arg1	Deletion					76:83	Deletion	76:83	Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes	76:155	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	4	84	theme	spore	865:869	arg1	layer					871:875	the outermost spore layer	851:875	the outermost spore layer	851:875	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	84	theme	spore	865:869	arg1	morphology					811:820	the morphology	807:820	the morphology	807:820	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	12	85	theme	various	2165:2171	arg1	habitats					2173:2180	various habitats	2165:2180	various habitats from natural to human-made environments	2165:2220	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	15	86	theme	sporulation	2574:2584	arg1	clusters					2591:2598	several sporulation gene clusters	2566:2598	several sporulation gene clusters	2566:2598	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	9	87	theme	own	1654:1656	arg1	deoxyhexose					1669:1679	its own respective deoxyhexose	1650:1679	its own respective deoxyhexose	1650:1679	Our data are consistent with the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose.
31235516	8	88	theme	paralogous	1396:1405	arg1	all					1414:1416	all	1414:1416	all	1414:1416	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	8	88	theme	paralogous	1396:1405	arg1	yfnH					1377:1380	yfnH	1377:1380	yfnH	1377:1380	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	8	88	theme	paralogous	1396:1405	arg1	genes					1407:1411	paralogous genes	1396:1411	paralogous genes	1396:1411	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	8	88	theme	paralogous	1396:1405	arg1	spsI					1371:1374	spsI	1371:1374	spsI	1371:1374	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	8	88	theme	paralogous	1396:1405	arg1	ytdA					1387:1390	ytdA	1387:1390	ytdA	1387:1390	While spsI, yfnH, and ytdA are paralogous genes, all encoding glucose-1-phosphate nucleotidyltransferases, each paralog appears to contribute in a distinct manner to the spore PS.
31235516	12	89	theme	spore	2146:2150	arg1	dispersal					2152:2160	spore dispersal	2146:2160	spore dispersal	2146:2160	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	13	90	theme	proteins	2302:2309	arg1	combination					2287:2297	a combination	2285:2297	a combination of proteins and polysaccharides	2285:2329	The spore surface in Bacillus subtilis (crust) is composed of a combination of proteins and polysaccharides.
31235516	9	91	theme	deoxyhexose	1669:1679	arg1	production					1636:1645	the production	1632:1645	the production of its own respective deoxyhexose	1632:1679	Our data are consistent with the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose.
31235516	0	92	theme	Spore	17:21	arg1	Layer					46:50	the Spore Surface Polysaccharide Layer	13:50	the Spore Surface Polysaccharide Layer	13:50	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	13	93	theme	polysaccharides	2315:2329	arg1	combination					2287:2297	a combination	2285:2297	a combination of proteins and polysaccharides	2285:2329	The spore surface in Bacillus subtilis (crust) is composed of a combination of proteins and polysaccharides.
31235516	0	94	theme	Polysaccharide	31:44	arg1	Layer					46:50	the Spore Surface Polysaccharide Layer	13:50	the Spore Surface Polysaccharide Layer	13:50	Expansion of the Spore Surface Polysaccharide Layer in Bacillus subtilis by Deletion of Genes Encoding Glycosyltransferases and Glucose Modification Enzymes.
31235516	13	95	theme	spore	2227:2231	arg1	surface					2233:2239	The spore surface	2223:2239	The spore surface in Bacillus subtilis (crust)	2223:2268	The spore surface in Bacillus subtilis (crust) is composed of a combination of proteins and polysaccharides.
31235516	7	96	from	spores	1271:1276	arg1	phenotype					1245:1253	The hydrophobic phenotype	1229:1253	The hydrophobic phenotype from sps mutant spores	1229:1276	The hydrophobic phenotype from sps mutant spores was partially rescued by a second mutation inactivating any gene in the yfnHGF operon.
31235516	3	97	theme	PS	373:374	arg1	enzymes					386:392	PS synthesis enzymes	373:392	PS synthesis enzymes produced during the late stages of sporulation	373:439	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	15	98	dep	assembly	2624:2631	arg1	the					2620:2622	the	2620:2622	the	2620:2622	Our findings indicate that several sporulation gene clusters are responsible for the assembly and allocation of surface polysaccharides.
31235516	3	99	theme	late	414:417	arg1	stages					419:424	the late stages	410:424	the late stages of sporulation	410:439	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	5	100	theme	PS	1049:1050	arg1	deposition					1052:1061	reduced PS deposition	1041:1061	reduced PS deposition	1041:1061	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
31235516	14	101	theme	spore	2434:2438	arg1	properties					2448:2457	spore surface properties	2434:2457	spore surface properties such as hydrophobicity	2434:2480	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	14	101	theme	spore	2434:2438	arg1	hydrophobicity					2467:2480	hydrophobicity	2467:2480	hydrophobicity	2467:2480	By inactivating the enzymes responsible for the synthesis of spore polysaccharides, we can assess how spore surface properties such as hydrophobicity are modulated by the addition of specific carbohydrates.
31235516	4	102	theme	adhesion	504:511	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	102	theme	adhesion	504:511	arg1	assays					536:541	bacterial adhesion to hydrocarbons (BATH) assays	494:541	bacterial adhesion to hydrocarbons (BATH) assays	494:541	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	10	103	theme	spore	1743:1747	arg1	hydrophobicity					1757:1770	spore surface hydrophobicity	1743:1770	spore surface hydrophobicity	1743:1770	In summary, we found that disruptions to the PS layer modify spore surface hydrophobicity and that there are multiple saccharide synthesis pathways involved in spore surface properties.IMPORTANCE Many bacteria are characterized by their ability to form highly resistant spores.
31235516	12	104	theme	surface	2078:2084	arg1	properties					2086:2095	Spore surface properties	2072:2095	Spore surface properties	2072:2095	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	3	105	theme	surface	454:460	arg1	properties					462:471	spore surface properties	448:471	spore surface properties	448:471	Here, we have assessed how PS synthesis enzymes produced during the late stages of sporulation affect spore surface properties.
31235516	4	106	with	microscopy	586:595	arg1	staining					622:629	ruthenium red staining	608:629	ruthenium red staining	608:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	107	theme	sporulation	715:725	arg1	clusters					732:739	four sporulation gene clusters	710:739	four sporulation gene clusters	710:739	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	107	theme	sporulation	715:725	arg1	ytdA-ytcABC					773:783	ytdA-ytcABC	773:783	ytdA-ytcABC	773:783	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	107	theme	sporulation	715:725	arg1	cgeAB-cgeCDE					790:801	cgeAB-cgeCDE	790:801	cgeAB-cgeCDE	790:801	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	107	theme	sporulation	715:725	arg1	yfnHGF-yfnED					759:770	yfnHGF-yfnED	759:770	yfnHGF-yfnED	759:770	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	107	theme	sporulation	715:725	arg1	spsABCDEFGHIJKL					742:756	spsABCDEFGHIJKL	742:756	spsABCDEFGHIJKL	742:756	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	12	108	theme	human-made	2198:2207	arg1	environments					2209:2220	human-made environments	2198:2220	human-made environments	2198:2220	Spore surface properties are particularly relevant because they influence spore dispersal in various habitats from natural to human-made environments.
31235516	4	109	theme	transmission	564:575	arg1	methods					485:491	four methods	480:491	four methods	480:491	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	109	theme	transmission	564:575	arg1	TEM					598:600	TEM	598:600	TEM	598:600	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	109	theme	transmission	564:575	arg1	microscopy					586:595	transmission electron microscopy	564:595	transmission electron microscopy (TEM) with ruthenium red staining	564:629	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	4	110	theme	clusters	732:739	arg1	contributions					693:705	the contributions	689:705	the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer	689:875	Using four methods, bacterial adhesion to hydrocarbons (BATH) assays, India ink staining, transmission electron microscopy (TEM) with ruthenium red staining, and scanning electron microscopy (SEM), we characterized the contributions of four sporulation gene clusters, spsABCDEFGHIJKL, yfnHGF-yfnED, ytdA-ytcABC, and cgeAB-cgeCDE, on the morphology and properties of the crust, the outermost spore layer.
31235516	9	111	theme	respective	1658:1667	arg1	deoxyhexose					1669:1679	its own respective deoxyhexose	1650:1679	its own respective deoxyhexose	1650:1679	Our data are consistent with the possibility that each gene cluster is responsible for the production of its own respective deoxyhexose.
31235516	5	112	theme	PS	1133:1134	arg1	layer					1136:1140	the PS layer	1129:1140	the PS layer	1129:1140	Our results show that all mutations in the sps operon result in the production of spores that are more hydrophobic and lack a visible crust, presumably because of reduced PS deposition, while mutations in cgeD and the yfnH-D cluster noticeably expand the PS layer.
29936201	0	0	theme	modified	84:91	arg1	chitosan					93:100	chitosan	93:100	chitosan	93:100	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	6	1	with	mixing	1117:1122	arg1	α-cyclodextrin					1144:1157	α-cyclodextrin	1144:1157	α-cyclodextrin in water	1144:1166	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	3	2	theme	palmitic	471:478	arg1	acid					480:483	palmitic acid	471:483	palmitic acid	471:483	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	4	3	theme	microscopy	563:572	arg1	experiments					695:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	5	4	theme	surface	889:895	arg1	analysis					868:875	AFM topographic analysis	852:875	AFM topographic analysis of platelet surface	852:895	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	0	5	from	insights	4:11	arg1	structure					20:28	the structure	16:28	the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	16:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	6	6	theme	simple	1101:1106	arg1	mixing					1117:1122	a simple magnetic mixing	1099:1122	a simple magnetic mixing of fatty acids with α-cyclodextrin in water	1099:1166	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	6	7	theme	fatty	1127:1131	arg1	acids					1133:1137	fatty acids	1127:1137	fatty acids	1127:1137	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	8	theme	electron	554:561	arg1	TEM					575:577	TEM	575:577	TEM	575:577	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	8	theme	electron	554:561	arg1	microscopy					563:572	Transmission electron microscopy	541:572	Transmission electron microscopy (TEM)	541:578	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	9	theme	cryogenic	581:589	arg1	TEM					591:593	cryogenic TEM	581:593	cryogenic TEM	581:593	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	5	10	theme	topographic	856:866	arg1	analysis					868:875	AFM topographic analysis	852:875	AFM topographic analysis of platelet surface	852:895	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	5	11	theme	12-14-nm	932:939	arg1	height					941:946	12-14-nm height	932:946	12-14-nm height	932:946	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	6	12	theme	solid	1181:1185	arg1	complexes					1197:1205	solid inclusion complexes	1181:1205	solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction	1181:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	13	theme	scanning	675:682	arg1	microscopy					684:693	confocal laser scanning microscopy	660:693	confocal laser scanning microscopy	660:693	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	5	14	theme	platelet	880:887	arg1	surface					889:895	platelet surface	880:895	platelet surface	880:895	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	4	15	theme	atomic	626:631	arg1	AFM					651:653	AFM	651:653	AFM	651:653	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	15	theme	atomic	626:631	arg1	microscopy					639:648	atomic force microscopy	626:648	atomic force microscopy (AFM)	626:654	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	16	theme	alkyl	806:810	arg1	chain					812:816	alkyl chain	806:816	alkyl chain grafted on chitosan	806:836	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	3	17	theme	fatty	426:430	arg1	acids					432:436	fatty acids	426:436	fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid)	426:500	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	0	18	from	structure	20:28	arg1	α-cyclodextrin					106:119	α-cyclodextrin	106:119	α-cyclodextrin	106:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	0	18	from	structure	20:28	arg1	chitosan					93:100	chitosan	93:100	chitosan	93:100	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	6	19	theme	X-ray	1274:1278	arg1	diffraction					1280:1290	powder X-ray diffraction	1267:1290	powder X-ray diffraction	1267:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	5	20	theme	thin	913:916	arg1	terraces					918:925	parallel thin terraces	904:925	parallel thin terraces with 12-14-nm height	904:946	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	4	21	theme	microscopy	639:648	arg1	experiments					695:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	0	22	theme	New	0:2	arg1	insights					4:11	New insights	0:11	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.	0:120	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	6	23	theme	powder	1267:1272	arg1	diffraction					1280:1290	powder X-ray diffraction	1267:1290	powder X-ray diffraction	1267:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	6	24	from	α-cyclodextrin	1144:1157	arg1	water					1162:1166	water	1162:1166	water	1162:1166	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	1	25	theme	research	190:197	arg1	group					199:203	our research group	186:203	our research group	186:203	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	7	26	theme	platelet	1385:1392	arg1	formation					1394:1402	platelet formation	1385:1402	platelet formation	1385:1402	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	1	27	from	α-cyclodextrin	261:274	arg1	water					279:283	water	279:283	water	279:283	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	5	28	theme	parallel	904:911	arg1	terraces					918:925	parallel thin terraces	904:925	parallel thin terraces with 12-14-nm height	904:946	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	3	29	theme	platelet	520:527	arg1	morphology					529:538	platelet morphology	520:538	platelet morphology	520:538	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	4	30	theme	scanning	596:603	arg1	microscopy					614:623	scanning electron microscopy	596:623	scanning electron microscopy	596:623	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	5	31	with	terraces	918:925	arg1	height					941:946	12-14-nm height	932:946	12-14-nm height	932:946	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	4	32	theme	Transmission	541:552	arg1	TEM					575:577	TEM	575:577	TEM	575:577	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	32	theme	Transmission	541:552	arg1	microscopy					563:572	Transmission electron microscopy	541:572	Transmission electron microscopy (TEM)	541:578	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	6	33	theme	inclusion	1187:1195	arg1	complexes					1197:1205	solid inclusion complexes	1181:1205	solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction	1181:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	2	34	theme	flat	300:303	arg1	surfaces					305:312	their flat surfaces	294:312	their flat surfaces	294:312	Because their flat surfaces, and according to their size, particles are called micro- or nano-platelets.
29936201	5	35	theme	AFM	852:854	arg1	analysis					868:875	AFM topographic analysis	852:875	AFM topographic analysis of platelet surface	852:895	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	7	36	theme	acid/α-cyclodextrin	1328:1346	arg1	interaction					1348:1358	fatty acid/α-cyclodextrin interaction	1322:1358	fatty acid/α-cyclodextrin interaction	1322:1358	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	7	36	theme	acid/α-cyclodextrin	1328:1346	arg1	force					1375:1379	the driving force	1363:1379	the driving force for platelet formation	1363:1402	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	4	37	theme	confocal	660:667	arg1	microscopy					684:693	confocal laser scanning microscopy	660:693	confocal laser scanning microscopy	660:693	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	1	38	from	acids	251:255	arg1	water					279:283	water	279:283	water	279:283	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	4	39	theme	microscopy	614:623	arg1	experiments					695:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	40	theme	force	633:637	arg1	AFM					651:653	AFM	651:653	AFM	651:653	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	40	theme	force	633:637	arg1	microscopy					639:648	atomic force microscopy	626:648	atomic force microscopy (AFM)	626:654	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	3	41	theme	acids	432:436	arg1	composition					411:421	the composition	407:421	the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid)	407:500	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	7	42	theme	fatty	1322:1326	arg1	interaction					1348:1358	fatty acid/α-cyclodextrin interaction	1322:1358	fatty acid/α-cyclodextrin interaction	1322:1358	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	7	42	theme	fatty	1322:1326	arg1	force					1375:1379	the driving force	1363:1379	the driving force for platelet formation	1363:1402	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	6	43	with	complexes	1197:1205	arg1	organization					1237:1248	a crystalline structural organization	1212:1248	a crystalline structural organization characterized by powder X-ray diffraction	1212:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	5	44	theme	fatty-acid/α-cyclodextrin	1011:1035	arg1	complexes					1047:1055	fatty-acid/α-cyclodextrin inclusion complexes	1011:1055	fatty-acid/α-cyclodextrin inclusion complexes	1011:1055	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	4	45	theme	electron	605:612	arg1	microscopy					614:623	scanning electron microscopy	596:623	scanning electron microscopy	596:623	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	0	46	theme	faceted	45:51	arg1	platelets					53:61	hexagonally faceted platelets	33:61	hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	33:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	5	47	theme	inclusion	1037:1045	arg1	complexes					1047:1055	fatty-acid/α-cyclodextrin inclusion complexes	1011:1055	fatty-acid/α-cyclodextrin inclusion complexes	1011:1055	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	6	48	theme	magnetic	1108:1115	arg1	mixing					1117:1122	a simple magnetic mixing	1099:1122	a simple magnetic mixing of fatty acids with α-cyclodextrin in water	1099:1166	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	49	theme	laser	669:673	arg1	microscopy					684:693	confocal laser scanning microscopy	660:693	confocal laser scanning microscopy	660:693	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	7	50	theme	driving	1367:1373	arg1	interaction					1348:1358	fatty acid/α-cyclodextrin interaction	1322:1358	fatty acid/α-cyclodextrin interaction	1322:1358	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	7	50	theme	driving	1367:1373	arg1	force					1375:1379	the driving force	1363:1379	the driving force for platelet formation	1363:1402	Our results demonstrate that fatty acid/α-cyclodextrin interaction is the driving force for platelet formation.
29936201	1	51	theme	particles	151:159	arg1	class					128:132	A new class	122:132	A new class of non-spherical particles	122:159	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	1	52	theme	new	124:126	arg1	class					128:132	A new class	122:132	A new class of non-spherical particles	122:159	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	6	53	theme	structural	1226:1235	arg1	organization					1237:1248	a crystalline structural organization	1212:1248	a crystalline structural organization characterized by powder X-ray diffraction	1212:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	54	contain	have	733:736	arg2	shape					765:769	a preferentially hexagonal shape	738:769	a preferentially hexagonal shape	738:769	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	4	54	contain	have	733:736	arg1	platelets					723:731	the platelets	719:731	the platelets	719:731	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	0	55	from	α-cyclodextrin	106:119	arg1	platelets					53:61	hexagonally faceted platelets	33:61	hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	33:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	0	55	from	α-cyclodextrin	106:119	arg1	structure					20:28	the structure	16:28	the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	16:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	5	56	theme	multi-layered	962:974	arg1	structure					976:984	a multi-layered structure	960:984	a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes	960:1055	Furthermore, AFM topographic analysis of platelet surface showed parallel thin terraces with 12-14-nm height, suggesting a multi-layered structure alternating chitosan and fatty-acid/α-cyclodextrin inclusion complexes.
29936201	4	57	theme	microscopy	684:693	arg1	experiments					695:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	6	58	theme	crystalline	1214:1224	arg1	organization					1237:1248	a crystalline structural organization	1212:1248	a crystalline structural organization characterized by powder X-ray diffraction	1212:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	59	theme	hexagonal	755:763	arg1	shape					765:769	a preferentially hexagonal shape	738:769	a preferentially hexagonal shape	738:769	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	3	60	theme	oleic	459:463	arg1	acid					465:468	oleic acid	459:468	oleic acid	459:468	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	6	61	theme	acids	1133:1137	arg1	mixing					1117:1122	a simple magnetic mixing	1099:1122	a simple magnetic mixing of fatty acids with α-cyclodextrin in water	1099:1166	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	0	62	from	chitosan	93:100	arg1	platelets					53:61	hexagonally faceted platelets	33:61	hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	33:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	0	62	from	chitosan	93:100	arg1	structure					20:28	the structure	16:28	the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	16:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	4	63	theme	sharp	776:780	arg1	edges					782:786	sharp edges	776:786	sharp edges	776:786	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	1	64	theme	non-spherical	137:149	arg1	particles					151:159	non-spherical particles	137:159	non-spherical particles	137:159	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
29936201	6	65	theme	first	1083:1087	arg1	time					1089:1092	the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction	1079:1290	the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction	1079:1290	We also revealed for the first time that a simple magnetic mixing of fatty acids with α-cyclodextrin in water results from solid inclusion complexes with a crystalline structural organization characterized by powder X-ray diffraction.
29936201	4	66	theme	TEM	591:593	arg1	experiments					695:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments	541:705	Transmission electron microscopy (TEM), cryogenic TEM, scanning electron microscopy, atomic force microscopy (AFM) and confocal laser scanning microscopy experiments showed that the platelets have a preferentially hexagonal shape with sharp edges, independently on alkyl chain grafted on chitosan.
29936201	0	67	theme	platelets	53:61	arg1	structure					20:28	the structure	16:28	the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin	16:119	New insights on the structure of hexagonally faceted platelets from hydrophobically modified chitosan and α-cyclodextrin.
29936201	3	68	dep	chitosan	449:456	arg1	acid					465:468	oleic acid	459:468	oleic acid	459:468	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	3	68	dep	chitosan	449:456	arg1	acid					480:483	palmitic acid	471:483	palmitic acid	471:483	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	3	68	dep	chitosan	449:456	arg1	acid					496:499	stearic acid	488:499	stearic acid	488:499	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	3	69	theme	stearic	488:494	arg1	acid					496:499	stearic acid	488:499	stearic acid	488:499	Here, we varied the composition of fatty acids grafted on chitosan (oleic acid, palmitic acid or stearic acid) and characterized platelet morphology.
29936201	1	70	theme	fatty	245:249	arg1	acids					251:255	fatty acids	245:255	fatty acids	245:255	A new class of non-spherical particles was recently designed in our research group by mixing a polysaccharide grafted with fatty acids and α-cyclodextrin in water.
30983428	10	0	theme	release	1691:1697	arg1	dispersion					1639:1648	nebulizable dispersion	1627:1648	nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1627:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	1	1	theme	low	265:267	arg1	%					294:294	6-8%	291:294	6-8%	291:294	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	1	theme	low	265:267	arg1	bioavailability					274:288	low oral bioavailability	265:288	low oral bioavailability (6-8%)	265:295	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	10	2	theme	effect	1727:1732	arg1	dispersion					1639:1648	nebulizable dispersion	1627:1648	nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1627:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	4	3	theme	composite	801:809	arg1	design					821:826	central composite rotatable design	793:826	central composite rotatable design	793:826	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	2	4	theme	chitosan-dextran	363:378	arg1	nanoparticles					388:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	The aim is to prepare cross-linked chitosan-dextran sulfate nanoparticles and/or nanodispersion.
30983428	3	5	theme	production	672:681	arg1	yield					683:687	% production yield	670:687	% production yield	670:687	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	6	theme	particle	558:565	arg1	size					567:570	mean particle size	553:570	mean particle size (MPS)	553:576	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	6	theme	particle	558:565	arg1	MPS					573:575	MPS	573:575	MPS	573:575	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	1	7	theme	BCS	203:205	arg1	Budesonide					187:196	Budesonide	187:196	Budesonide	187:196	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	7	theme	BCS	203:205	arg1	drug					216:219	a BCS class II drug	201:219	a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%)	201:295	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	0	8	theme	chitosan-dextran	86:101	arg1	nanodispersion					111:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	7	9	theme	emitted	1246:1252	arg1	drug					1254:1257	The emitted drug	1242:1257	The emitted drug in target region (alveoli)	1242:1284	The emitted drug in target region (alveoli) was measured by using HPLC and it was found to be 18.26%.
30983428	4	10	theme	drug	1024:1027	arg1	CDR					1006:1008	% CDR	1004:1008	% CDR (% cumulative drug release)	1004:1036	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	10	theme	drug	1024:1027	arg1	release					1029:1035	% cumulative drug release	1011:1035	% cumulative drug release	1011:1035	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	3	11	theme	polydispersity	579:592	arg1	PDI					601:603	PDI	601:603	PDI	601:603	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	11	theme	polydispersity	579:592	arg1	index					594:598	polydispersity index	579:598	polydispersity index (PDI)	579:604	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	1	12	theme	water	230:234	arg1	0.045 mg/mL					248:258	0.045 mg/mL	248:258	0.045 mg/mL	248:258	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	12	theme	water	230:234	arg1	solubility					236:245	low water solubility	226:245	low water solubility (0.045 mg/mL)	226:259	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	4	13	theme	independent	851:861	arg1	variables					863:871	independent variables	851:871	independent variables	851:871	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	10	14	link	cross-linked	1547:1558	arg1	nanoparticles					1585:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	6	15	theme	percent	1172:1178	arg1	loading					1185:1191	The percent drug loading	1168:1191	The percent drug loading of all the batches	1168:1210	The percent drug loading of all the batches was found in range of 10-16%.
30983428	10	16	theme	chitosan-dextran	1560:1575	arg1	nanoparticles					1585:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	9	17	theme	three	1511:1515	arg1	month					1517:1521	month	1517:1521	month	1517:1521	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	0	18	theme	cross-linked	73:84	arg1	nanodispersion					111:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	4	19	from	effect	841:846	arg1	Y1					977:978	Y1	977:978	Y1	977:978	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	19	from	effect	841:846	arg1	variables					961:969	the dependent variables	947:969	the dependent variables	947:969	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	3	20	theme	zeta	607:610	arg1	ZP					623:624	ZP	623:624	ZP	623:624	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	20	theme	zeta	607:610	arg1	potential					612:620	zeta potential	607:620	zeta potential (ZP)	607:625	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	0	21	theme	sulfate	103:109	arg1	nanodispersion					111:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	4	22	theme	concentration	909:921	arg1	sulfate					931:937	concentration dextran sulfate	909:937	concentration dextran sulfate (X2)	909:942	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	22	theme	concentration	909:921	arg1	X2					940:941	X2	940:941	X2	940:941	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	6	23	theme	batches	1204:1210	arg1	loading					1185:1191	The percent drug loading	1168:1191	The percent drug loading of all the batches	1168:1210	The percent drug loading of all the batches was found in range of 10-16%.
30983428	8	24	dep	in	1394:1395	arg1	vitro					1397:1401	vitro	1397:1401	vitro	1397:1401	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	4	25	theme	sulfate	931:937	arg1	effect					841:846	the effect	837:846	the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3)	837:1041	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	10	26	theme	pass	1722:1725	arg1	effect					1727:1732	the first pass effect	1712:1732	avoiding the first pass effect	1703:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	3	27	theme	cross-linked	437:448	arg1	nanodispersion					450:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	0	28	theme	Central	0:6	arg1	design					28:33	Central composite rotatable design	0:33	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	0:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	28	theme	Central	0:6	arg1	diffusion					154:162	in vitro diffusion and aerodynamic study	145:184	diffusion	154:162	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	28	theme	Central	0:6	arg1	study					180:184	in vitro diffusion and aerodynamic study	145:184	study	180:184	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	3	29	link	cross-linked	437:448	arg1	nanodispersion					450:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	2	30	link	cross-linked	350:361	arg1	nanoparticles					388:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	The aim is to prepare cross-linked chitosan-dextran sulfate nanoparticles and/or nanodispersion.
30983428	3	31	theme	solvent	485:491	arg1	technique					505:513	the solvent evaporation technique	481:513	the solvent evaporation technique	481:513	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	0	32	theme	rotatable	18:26	arg1	design					28:33	Central composite rotatable design	0:33	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	0:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	32	theme	rotatable	18:26	arg1	diffusion					154:162	in vitro diffusion and aerodynamic study	145:184	diffusion	154:162	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	32	theme	rotatable	18:26	arg1	study					180:184	in vitro diffusion and aerodynamic study	145:184	study	180:184	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	4	33	theme	%	1011:1011	arg1	CDR					1006:1008	% CDR	1004:1008	% CDR (% cumulative drug release)	1004:1036	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	33	theme	%	1011:1011	arg1	release					1029:1035	% cumulative drug release	1011:1035	% cumulative drug release	1011:1035	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	3	34	theme	drug	628:631	arg1	loading					633:639	drug loading	628:639	drug loading	628:639	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	4	35	theme	dependent	951:959	arg1	Y1					977:978	Y1	977:978	Y1	977:978	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	35	theme	dependent	951:959	arg1	variables					961:969	the dependent variables	947:969	the dependent variables	947:969	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	9	36	theme	month	1517:1521	arg1	storage					1523:1529	three month storage	1511:1529	three month storage	1511:1529	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	10	37	theme	first	1716:1720	arg1	effect					1727:1732	the first pass effect	1712:1732	avoiding the first pass effect	1703:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	10	38	theme	increased	1653:1661	arg1	loading					1668:1674	increased drug loading	1653:1674	increased drug loading	1653:1674	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	1	39	theme	high	304:307	arg1	pass					315:318	high first pass	304:318	high first pass effect	304:325	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	40	theme	pass	315:318	arg1	effect					320:325	high first pass effect	304:325	high first pass effect	304:325	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	10	41	theme	in	1677:1678	arg1	release					1691:1697	in vitro drug release	1677:1697	in vitro drug release	1677:1697	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	8	42	theme	optimum	1386:1392	arg1	behavior					1415:1422	the optimum in vitro aerodynamic behavior	1382:1422	the optimum in vitro aerodynamic behavior	1382:1422	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	10	43	theme	loading	1668:1674	arg1	dispersion					1639:1648	nebulizable dispersion	1627:1648	nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1627:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	4	44	theme	formulation	763:773	arg1	optimization					747:758	The optimization	743:758	The optimization of formulation	743:773	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	5	45	theme	nanoparticles	1086:1098	arg1	PDI					1053:1055	PDI	1053:1055	PDI	1053:1055	The MPS, PDI, and ZP of budesonide-loaded nanoparticles were 160.8 ± 0.27 nm, 0.36 ± 0.04, and 13 ± 0.894 mV, respectively.
30983428	5	45	theme	nanoparticles	1086:1098	arg1	ZP					1062:1063	ZP	1062:1063	ZP	1062:1063	The MPS, PDI, and ZP of budesonide-loaded nanoparticles were 160.8 ± 0.27 nm, 0.36 ± 0.04, and 13 ± 0.894 mV, respectively.
30983428	5	45	theme	nanoparticles	1086:1098	arg1	MPS					1048:1050	MPS	1048:1050	MPS	1048:1050	The MPS, PDI, and ZP of budesonide-loaded nanoparticles were 160.8 ± 0.27 nm, 0.36 ± 0.04, and 13 ± 0.894 mV, respectively.
30983428	9	46	theme	study	1435:1439	arg1	results					1441:1447	Stability study results	1425:1447	Stability study results	1425:1447	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	10	47	dep	in	1677:1678	arg1	vitro					1680:1684	vitro	1680:1684	vitro	1680:1684	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	3	48	theme	%	670:670	arg1	yield					683:687	% production yield	670:687	% production yield	670:687	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	6	49	located	found	1216:1220	arg2	loading					1185:1191	The percent drug loading	1168:1191	The percent drug loading of all the batches	1168:1210	The percent drug loading of all the batches was found in range of 10-16%.
30983428	6	49	located	found	1216:1220	arg1	range					1225:1229	range	1225:1229	range of 10-16%	1225:1239	The percent drug loading of all the batches was found in range of 10-16%.
30983428	4	50	theme	drug	982:985	arg1	Y2					996:997	Y2	996:997	Y2	996:997	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	50	theme	drug	982:985	arg1	loading					987:993	drug loading	982:993	drug loading (Y2)	982:998	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	10	51	theme	drug	1686:1689	arg1	release					1691:1697	in vitro drug release	1677:1697	in vitro drug release	1677:1697	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	1	52	theme	oral	269:272	arg1	%					294:294	6-8%	291:294	6-8%	291:294	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	52	theme	oral	269:272	arg1	bioavailability					274:288	low oral bioavailability	265:288	low oral bioavailability (6-8%)	265:295	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	9	53	theme	significant	1459:1469	arg1	change					1471:1476	no significant change	1456:1476	no significant change in MPS, PDI, ZP, and % CDR	1456:1503	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	4	54	theme	cumulative	1013:1022	arg1	CDR					1006:1008	% CDR	1004:1008	% CDR (% cumulative drug release)	1004:1036	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	54	theme	cumulative	1013:1022	arg1	release					1029:1035	% cumulative drug release	1011:1035	% cumulative drug release	1011:1035	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	3	55	theme	in	690:691	arg1	diffusion					699:707	in vitro diffusion	690:707	in vitro diffusion	690:707	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	56	theme	mean	553:556	arg1	size					567:570	mean particle size	553:570	mean particle size (MPS)	553:576	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	56	theme	mean	553:556	arg1	MPS					573:575	MPS	573:575	MPS	573:575	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	4	57	theme	central	793:799	arg1	design					821:826	central composite rotatable design	793:826	central composite rotatable design	793:826	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	7	58	theme	target	1262:1267	arg1	alveoli					1277:1283	alveoli	1277:1283	alveoli	1277:1283	The emitted drug in target region (alveoli) was measured by using HPLC and it was found to be 18.26%.
30983428	7	58	theme	target	1262:1267	arg1	region					1269:1274	target region	1262:1274	target region (alveoli)	1262:1284	The emitted drug in target region (alveoli) was measured by using HPLC and it was found to be 18.26%.
30983428	10	59	theme	cross-linked	1547:1558	arg1	nanoparticles					1585:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	1	60	theme	class	207:211	arg1	Budesonide					187:196	Budesonide	187:196	Budesonide	187:196	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	60	theme	class	207:211	arg1	drug					216:219	a BCS class II drug	201:219	a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%)	201:295	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	4	61	theme	rotatable	811:819	arg1	design					821:826	central composite rotatable design	793:826	central composite rotatable design	793:826	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	62	theme	%	1004:1004	arg1	CDR					1006:1008	% CDR	1004:1008	% CDR (% cumulative drug release)	1004:1036	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	62	theme	%	1004:1004	arg1	release					1029:1035	% cumulative drug release	1011:1035	% cumulative drug release	1011:1035	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	9	63	from	change	1471:1476	arg1	ZP					1491:1492	ZP	1491:1492	ZP	1491:1492	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	9	63	from	change	1471:1476	arg1	MPS					1481:1483	MPS	1481:1483	MPS	1481:1483	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	9	63	from	change	1471:1476	arg1	PDI					1486:1488	PDI	1486:1488	PDI	1486:1488	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	9	63	from	change	1471:1476	arg1	CDR					1501:1503	% CDR	1499:1503	% CDR	1499:1503	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	4	64	theme	concentration	874:886	arg1	effect					841:846	the effect	837:846	the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3)	837:1041	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	0	65	theme	aerodynamic	168:178	arg1	design					28:33	Central composite rotatable design	0:33	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	0:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	65	theme	aerodynamic	168:178	arg1	study					180:184	in vitro diffusion and aerodynamic study	145:184	study	180:184	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	1	66	theme	low	226:228	arg1	0.045 mg/mL					248:258	0.045 mg/mL	248:258	0.045 mg/mL	248:258	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	66	theme	low	226:228	arg1	solubility					236:245	low water solubility	226:245	low water solubility (0.045 mg/mL)	226:259	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	4	67	theme	chitosan	891:898	arg1	variables					863:871	independent variables	851:871	independent variables	851:871	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	67	theme	chitosan	891:898	arg1	sulfate					931:937	concentration dextran sulfate	909:937	concentration dextran sulfate (X2)	909:942	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	67	theme	chitosan	891:898	arg1	X1					901:902	X1	901:902	X1	901:902	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	67	theme	chitosan	891:898	arg1	concentration					874:886	concentration	874:886	concentration of chitosan (X1)	874:903	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	67	theme	chitosan	891:898	arg1	X2					940:941	X2	940:941	X2	940:941	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	8	68	contain	had	1378:1380	arg1	nanodispersion					1363:1376	nanodispersion	1363:1376	nanodispersion	1363:1376	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	8	68	contain	had	1378:1380	arg2	behavior					1415:1422	the optimum in vitro aerodynamic behavior	1382:1422	the optimum in vitro aerodynamic behavior	1382:1422	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	1	69	theme	first	309:313	arg1	pass					315:318	high first pass	304:318	high first pass effect	304:325	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	6	70	theme	drug	1180:1183	arg1	loading					1185:1191	The percent drug loading	1168:1191	The percent drug loading of all the batches	1168:1210	The percent drug loading of all the batches was found in range of 10-16%.
30983428	10	71	theme	sulfate	1577:1583	arg1	nanoparticles					1585:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	9	72	theme	%	1499:1499	arg1	CDR					1501:1503	% CDR	1499:1503	% CDR	1499:1503	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	10	73	theme	nebulizable	1627:1637	arg1	dispersion					1639:1648	nebulizable dispersion	1627:1648	nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1627:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	4	74	theme	variables	863:871	arg1	effect					841:846	the effect	837:846	the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3)	837:1041	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	6	75	theme	%	1239:1239	arg1	range					1225:1229	range	1225:1229	range of 10-16%	1225:1239	The percent drug loading of all the batches was found in range of 10-16%.
30983428	10	76	theme	suitable	1614:1621	arg1	properties					1603:1612	properties	1603:1612	properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1603:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	7	77	from	drug	1254:1257	arg1	alveoli					1277:1283	alveoli	1277:1283	alveoli	1277:1283	The emitted drug in target region (alveoli) was measured by using HPLC and it was found to be 18.26%.
30983428	7	77	from	drug	1254:1257	arg1	region					1269:1274	target region	1262:1274	target region (alveoli)	1262:1284	The emitted drug in target region (alveoli) was measured by using HPLC and it was found to be 18.26%.
30983428	4	78	theme	dextran	923:929	arg1	sulfate					931:937	concentration dextran sulfate	909:937	concentration dextran sulfate (X2)	909:942	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	78	theme	dextran	923:929	arg1	X2					940:941	X2	940:941	X2	940:941	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	5	79	dep	MPS	1048:1050	arg1	The					1044:1046	The	1044:1046	The	1044:1046	The MPS, PDI, and ZP of budesonide-loaded nanoparticles were 160.8 ± 0.27 nm, 0.36 ± 0.04, and 13 ± 0.894 mV, respectively.
30983428	0	80	theme	nanodispersion	111:124	arg1	optimization					39:50	optimization	39:50	optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	39:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	81	dep	in	145:146	arg1	vitro					148:152	vitro	148:152	vitro	148:152	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	82	theme	in	145:146	arg1	design					28:33	Central composite rotatable design	0:33	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	0:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	82	theme	in	145:146	arg1	diffusion					154:162	in vitro diffusion and aerodynamic study	145:184	diffusion	154:162	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	4	83	dep	Y1	977:978	arg1	CDR					1006:1008	% CDR	1004:1008	% CDR (% cumulative drug release)	1004:1036	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	83	dep	Y1	977:978	arg1	Y2					996:997	Y2	996:997	Y2	996:997	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	83	dep	Y1	977:978	arg1	loading					987:993	drug loading	982:993	drug loading (Y2)	982:998	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	4	83	dep	Y1	977:978	arg1	release					1029:1035	% cumulative drug release	1011:1035	% cumulative drug release	1011:1035	The optimization of formulation was done by using central composite rotatable design to study the effect of independent variables, concentration of chitosan (X1) and concentration dextran sulfate (X2) on the dependent variables, MPS (Y1), drug loading (Y2) and % CDR (% cumulative drug release) (Y3).
30983428	2	84	theme	sulfate	380:386	arg1	nanoparticles					388:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	The aim is to prepare cross-linked chitosan-dextran sulfate nanoparticles and/or nanodispersion.
30983428	0	85	theme	composite	8:16	arg1	design					28:33	Central composite rotatable design	0:33	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	0:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	85	theme	composite	8:16	arg1	diffusion					154:162	in vitro diffusion and aerodynamic study	145:184	diffusion	154:162	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	85	theme	composite	8:16	arg1	study					180:184	in vitro diffusion and aerodynamic study	145:184	study	180:184	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	3	86	dep	XRPD	541:544	arg1	study					736:740	study	736:740	study	736:740	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	2	87	theme	cross-linked	350:361	arg1	nanoparticles					388:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	cross-linked chitosan-dextran sulfate nanoparticles	350:400	The aim is to prepare cross-linked chitosan-dextran sulfate nanoparticles and/or nanodispersion.
30983428	10	88	contain	had	1599:1601	arg1	nanoparticles					1585:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	cross-linked chitosan-dextran sulfate nanoparticles	1547:1597	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	10	88	contain	had	1599:1601	arg2	properties					1603:1612	properties	1603:1612	properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect	1603:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	10	89	theme	drug	1663:1666	arg1	loading					1668:1674	increased drug loading	1653:1674	increased drug loading	1653:1674	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30983428	3	90	theme	evaporation	493:503	arg1	technique					505:513	the solvent evaporation technique	481:513	the solvent evaporation technique	481:513	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	1	91	with	drug	216:219	arg1	%					294:294	6-8%	291:294	6-8%	291:294	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	91	with	drug	216:219	arg1	0.045 mg/mL					248:258	0.045 mg/mL	248:258	0.045 mg/mL	248:258	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	91	with	drug	216:219	arg1	solubility					236:245	low water solubility	226:245	low water solubility (0.045 mg/mL)	226:259	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	1	91	with	drug	216:219	arg1	bioavailability					274:288	low oral bioavailability	265:288	low oral bioavailability (6-8%)	265:295	Budesonide is a BCS class II drug with low water solubility (0.045 mg/mL) and low oral bioavailability (6-8%) due to high first pass effect.
30983428	3	92	theme	Nebulizable	425:435	arg1	nanodispersion					450:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion	425:463	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	0	93	theme	budesonide-loaded	55:71	arg1	nanodispersion					111:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	3	94	theme	entrapment	642:651	arg1	efficiency					653:662	entrapment efficiency	642:662	entrapment efficiency	642:662	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	3	95	dep	in	690:691	arg1	vitro					693:697	vitro	693:697	vitro	693:697	Nebulizable cross-linked nanodispersion was prepared by the solvent evaporation technique and characterized through XRPD, FTIR, mean particle size (MPS), polydispersity index (PDI), zeta potential (ZP), drug loading, entrapment efficiency, SEM, % production yield, in vitro diffusion, aerodynamic and stability study.
30983428	0	96	dep	design	28:33	arg1	characterization					127:142	characterization	127:142	characterization	127:142	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	0	97	link	cross-linked	73:84	arg1	nanodispersion					111:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion	55:124	Central composite rotatable design for optimization of budesonide-loaded cross-linked chitosan-dextran sulfate nanodispersion: characterization, in vitro diffusion and aerodynamic study.
30983428	8	98	theme	in	1394:1395	arg1	behavior					1415:1422	the optimum in vitro aerodynamic behavior	1382:1422	the optimum in vitro aerodynamic behavior	1382:1422	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	5	99	theme	budesonide-loaded	1068:1084	arg1	nanoparticles					1086:1098	budesonide-loaded nanoparticles	1068:1098	budesonide-loaded nanoparticles	1068:1098	The MPS, PDI, and ZP of budesonide-loaded nanoparticles were 160.8 ± 0.27 nm, 0.36 ± 0.04, and 13 ± 0.894 mV, respectively.
30983428	9	100	theme	Stability	1425:1433	arg1	results					1441:1447	Stability study results	1425:1447	Stability study results	1425:1447	Stability study results showed no significant change in MPS, PDI, ZP, and % CDR after three month storage.
30983428	8	101	theme	aerodynamic	1403:1413	arg1	behavior					1415:1422	the optimum in vitro aerodynamic behavior	1382:1422	the optimum in vitro aerodynamic behavior	1382:1422	It was found that, nanodispersion had the optimum in vitro aerodynamic behavior.
30983428	10	102	theme	avoiding	1703:1710	arg1	effect					1727:1732	the first pass effect	1712:1732	avoiding the first pass effect	1703:1732	In conclusion, cross-linked chitosan-dextran sulfate nanoparticles had properties suitable for nebulizable dispersion of increased drug loading, in vitro drug release and avoiding the first pass effect.
30055911	0	0	theme	weight	82:87	arg1	heparins					89:96	low molecular weight heparins	68:96	low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	68:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	1	1	theme	lower	330:334	arg1	effects					341:347	lower side effects	330:347	lower side effects	330:347	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	0	2	theme	spectrometry	192:203	arg1	time					172:175	high performance anion exchange chromatography hyphenated quadrupole time	103:175	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	3	3	theme	nitrous	489:495	arg1	degradation					502:512	nitrous acid degradation	489:512	nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains	489:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	14	4	theme	An-Man	1898:1903	arg1	determination					1881:1893	determination	1881:1893	determination of An-Man	1881:1903	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	6	5	theme	nonvolatile	971:981	arg1	salts					983:987	the nonvolatile salts	967:987	the nonvolatile salts from HPAEC	967:998	An ion suppressor is set up between HPAEC and MS to remove the nonvolatile salts from HPAEC and make the elute compatible to MS. Various monosaccharides were separated well with HPAEC.
30055911	0	6	theme	molecular	72:80	arg1	heparins					89:96	low molecular weight heparins	68:96	low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	68:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	1	7	theme	side	336:339	arg1	effects					341:347	lower side effects	330:347	lower side effects	330:347	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	0	8	theme	mass	187:190	arg1	spectrometry					192:203	flight mass spectrometry	180:203	flight mass spectrometry	180:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	11	9	theme	glucosamine	1504:1514	arg1	ratio					1495:1499	The ratio	1491:1499	The ratio of glucosamine to An-Man	1491:1524	The ratio of glucosamine to An-Man could be used to calculate the molecular weight of LMWHs.
30055911	3	10	theme	acid	497:500	arg1	degradation					502:512	nitrous acid degradation	489:512	nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains	489:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	12	11	theme	chromatography-multiple	1663:1685	arg1	scattering					1699:1708	size-exclusion chromatography-multiple angle laser scattering	1648:1708	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	1	12	theme	unfractionated	258:271	arg1	UFH					282:284	UFH	282:284	UFH	282:284	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	12	theme	unfractionated	258:271	arg1	heparin					273:279	unfractionated heparin	258:279	unfractionated heparin (UFH)	258:285	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	7	13	theme	heparin	1165:1171	arg1	samples					1173:1179	heparin samples	1165:1179	heparin samples	1165:1179	Online MS and MS/MS confirmed all sugar residues in the hydrolysates of heparin samples.
30055911	3	14	theme	chains	673:678	arg1	terminals					644:652	the reducing terminals	631:652	the reducing terminals of generated sugar chains	631:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	15	15	theme	alternative	1920:1930	arg1	method					1932:1937	an alternative method	1917:1937	an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes	1917:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	15	15	theme	alternative	1920:1930	arg1	It					1906:1907	It	1906:1907	It	1906:1907	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	3	16	theme	specific	567:574	arg1	2,5-anhydro-d-mannitol					585:606	2,5-anhydro-d-mannitol	585:606	2,5-anhydro-d-mannitol (An-Man)	585:615	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	3	16	theme	specific	567:574	arg1	residue					576:582	specific residue	567:582	specific residue	567:582	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	3	17	theme	subsequent	528:537	arg1	process					549:555	a subsequent reduction process	526:555	a subsequent reduction process	526:555	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	12	18	theme	size-exclusion	1648:1661	arg1	scattering					1699:1708	size-exclusion chromatography-multiple angle laser scattering	1648:1708	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	7	19	theme	samples	1173:1179	arg1	hydrolysates					1149:1160	the hydrolysates	1145:1160	the hydrolysates of heparin samples	1145:1179	Online MS and MS/MS confirmed all sugar residues in the hydrolysates of heparin samples.
30055911	3	20	theme	sugar	667:671	arg1	chains					673:678	generated sugar chains	657:678	generated sugar chains	657:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	6	21	from	HPAEC	994:998	arg1	salts					983:987	the nonvolatile salts	967:987	the nonvolatile salts from HPAEC	967:998	An ion suppressor is set up between HPAEC and MS to remove the nonvolatile salts from HPAEC and make the elute compatible to MS. Various monosaccharides were separated well with HPAEC.
30055911	3	22	theme	reduction	539:547	arg1	process					549:555	a subsequent reduction process	526:555	a subsequent reduction process	526:555	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	15	23	theme	LMWHs	1960:1964	arg1	MWs					1953:1955	the MWs	1949:1955	the MWs of LMWHs having An-Man for quality control purposes	1949:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	4	24	theme	practicable	694:704	arg1	method					717:722	few practicable analytical method	690:722	few practicable analytical method	690:722	However, few practicable analytical method was available to analyze An-Man qualitatively and quantitatively.
30055911	0	25	theme	performance	108:118	arg1	chromatography					135:148	high performance anion exchange chromatography	103:148	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	7	26	from	residues	1133:1140	arg1	hydrolysates					1149:1160	the hydrolysates	1145:1160	the hydrolysates of heparin samples	1145:1179	Online MS and MS/MS confirmed all sugar residues in the hydrolysates of heparin samples.
30055911	10	27	theme	residues	1439:1446	arg1	amounts					1416:1422	The amounts	1412:1422	The amounts of these sugar residues	1412:1446	The amounts of these sugar residues were quantitated with PAD, simultaneously.
30055911	10	27	theme	residues	1439:1446	arg1	residues					1439:1446	these sugar residues	1427:1446	these sugar residues	1427:1446	The amounts of these sugar residues were quantitated with PAD, simultaneously.
30055911	9	28	theme	heparin	1395:1401	arg1	samples					1403:1409	all heparin samples	1391:1409	all heparin samples	1391:1409	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	0	29	theme	high	103:106	arg1	chromatography					135:148	high performance anion exchange chromatography	103:148	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	8	30	located	observed	1221:1228	arg2	residue					1195:1201	The specific residue	1182:1201	The specific residue	1182:1201	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	8	30	located	observed	1221:1228	arg1	hydrolysates					1237:1248	the hydrolysates	1233:1248	the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction	1233:1310	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	8	30	located	observed	1221:1228	arg2	An-Man					1204:1209	An-Man	1204:1209	An-Man	1204:1209	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	6	31	theme	ion	911:913	arg1	suppressor					915:924	An ion suppressor	908:924	An ion suppressor	908:924	An ion suppressor is set up between HPAEC and MS to remove the nonvolatile salts from HPAEC and make the elute compatible to MS. Various monosaccharides were separated well with HPAEC.
30055911	2	32	theme	used	430:433	arg1	nadroparin					383:392	nadroparin	383:392	nadroparin	383:392	Enoxaparin, nadroparin, and dalteparin are the most widely used LMWHs.
30055911	2	32	theme	used	430:433	arg1	dalteparin					399:408	dalteparin	399:408	dalteparin	399:408	Enoxaparin, nadroparin, and dalteparin are the most widely used LMWHs.
30055911	2	32	theme	used	430:433	arg1	Enoxaparin					371:380	Enoxaparin	371:380	Enoxaparin	371:380	Enoxaparin, nadroparin, and dalteparin are the most widely used LMWHs.
30055911	2	32	theme	used	430:433	arg1	LMWHs					435:439	the most widely used LMWHs	414:439	the most widely used LMWHs	414:439	Enoxaparin, nadroparin, and dalteparin are the most widely used LMWHs.
30055911	9	33	located	observed	1379:1386	arg2	glucosamine					1332:1342	major glucosamine	1326:1342	major glucosamine	1326:1342	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	9	33	located	observed	1379:1386	arg1	samples					1403:1409	all heparin samples	1391:1409	all heparin samples	1391:1409	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	9	33	located	observed	1379:1386	arg1	addition					1316:1323	addition	1316:1323	addition	1316:1323	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	9	33	located	observed	1379:1386	arg2	arabinose/galactose					1354:1372	minor arabinose/galactose	1348:1372	minor arabinose/galactose	1348:1372	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	13	34	dep	weight	1784:1789	arg1	higher					1763:1768	higher	1763:1768	higher	1763:1768	Higher the ratio of glucosamine to An-Man higher the molecular weight.
30055911	0	35	theme	Qualitative	0:10	arg1	analysis					29:36	Qualitative and quantitative analysis	0:36	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.	0:204	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	15	36	theme	quality	1984:1990	arg1	purposes					2000:2007	quality control purposes	1984:2007	quality control purposes	1984:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	13	37	dep	Higher	1721:1726	arg1	ratio					1732:1736	the ratio	1728:1736	Higher the ratio of glucosamine to An-Man	1721:1761	Higher the ratio of glucosamine to An-Man higher the molecular weight.
30055911	12	38	theme	calculated	1588:1597	arg1	values					1599:1604	The calculated values	1584:1604	The calculated values	1584:1604	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	12	38	theme	calculated	1588:1597	arg1	comparable					1610:1619	comparable	1610:1619	comparable	1610:1619	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	1	39	theme	Low	206:208	arg1	LMWHs					237:241	LMWHs	237:241	LMWHs	237:241	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	39	theme	Low	206:208	arg1	heparins					227:234	Low molecular weight heparins	206:234	Low molecular weight heparins (LMWHs)	206:242	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	7	40	theme	sugar	1127:1131	arg1	residues					1133:1140	all sugar residues	1123:1140	all sugar residues in the hydrolysates of heparin samples	1123:1179	Online MS and MS/MS confirmed all sugar residues in the hydrolysates of heparin samples.
30055911	14	41	theme	HPAEC-PAD-MS	1796:1807	arg1	way					1856:1858	an accurate, precise and efficient way	1821:1858	an accurate, precise and efficient way to identify LMWHs by determination of An-Man	1821:1903	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	14	41	theme	HPAEC-PAD-MS	1796:1807	arg1	platform					1809:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	0	42	theme	exchange	126:133	arg1	chromatography					135:148	high performance anion exchange chromatography	103:148	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	0	43	with	heparins	89:96	arg1	time					172:175	high performance anion exchange chromatography hyphenated quadrupole time	103:175	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	13	44	theme	molecular	1774:1782	arg1	weight					1784:1789	the molecular weight	1770:1789	the molecular weight	1770:1789	Higher the ratio of glucosamine to An-Man higher the molecular weight.
30055911	15	45	contain	having	1966:1971	arg2	An-Man					1973:1978	An-Man	1973:1978	An-Man for quality control purposes	1973:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	15	45	contain	having	1966:1971	arg1	LMWHs					1960:1964	LMWHs	1960:1964	LMWHs having An-Man for quality control purposes	1960:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	1	46	theme	molecular	210:218	arg1	LMWHs					237:241	LMWHs	237:241	LMWHs	237:241	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	46	theme	molecular	210:218	arg1	heparins					227:234	Low molecular weight heparins	206:234	Low molecular weight heparins (LMWHs)	206:242	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	0	47	theme	quantitative	16:27	arg1	analysis					29:36	Qualitative and quantitative analysis	0:36	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.	0:204	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	0	48	theme	anion	120:124	arg1	chromatography					135:148	high performance anion exchange chromatography	103:148	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	8	49	theme	acid	1281:1284	arg1	degradation					1286:1296	nitrous acid degradation	1273:1296	nitrous acid degradation	1273:1296	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	1	50	theme	weight	220:225	arg1	LMWHs					237:241	LMWHs	237:241	LMWHs	237:241	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	50	theme	weight	220:225	arg1	heparins					227:234	Low molecular weight heparins	206:234	Low molecular weight heparins (LMWHs)	206:242	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	0	51	theme	2	41:41	arg1	analysis					29:36	Qualitative and quantitative analysis	0:36	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.	0:204	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	1	52	theme	chemical	290:297	arg1	degradation					312:322	chemical or enzymatic degradation	290:322	chemical or enzymatic degradation	290:322	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	0	53	theme	hyphenated	150:159	arg1	time					172:175	high performance anion exchange chromatography hyphenated quadrupole time	103:175	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	12	54	theme	scattering	1699:1708	arg1	detection					1710:1718	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	15	55	theme	control	1992:1998	arg1	purposes					2000:2007	quality control purposes	1984:2007	quality control purposes	1984:2007	It is also an alternative method to detect the MWs of LMWHs having An-Man for quality control purposes.
30055911	7	56	theme	Online	1093:1098	arg1	MS					1100:1101	Online MS	1093:1101	Online MS	1093:1101	Online MS and MS/MS confirmed all sugar residues in the hydrolysates of heparin samples.
30055911	0	57	theme	chromatography	135:148	arg1	time					172:175	high performance anion exchange chromatography hyphenated quadrupole time	103:175	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	8	58	theme	LMWHs	1253:1257	arg1	hydrolysates					1237:1248	the hydrolysates	1233:1248	the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction	1233:1310	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	1	59	theme	enzymatic	302:310	arg1	degradation					312:322	chemical or enzymatic degradation	290:322	chemical or enzymatic degradation	290:322	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	60	attach	derived	245:251	arg2	LMWHs					237:241	LMWHs	237:241	LMWHs	237:241	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	60	attach	derived	245:251	arg1	UFH					282:284	UFH	282:284	UFH	282:284	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	60	attach	derived	245:251	arg2	heparins					227:234	Low molecular weight heparins	206:234	Low molecular weight heparins (LMWHs)	206:242	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	60	attach	derived	245:251	arg1	heparin					273:279	unfractionated heparin	258:279	unfractionated heparin (UFH)	258:285	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	11	61	theme	molecular	1557:1565	arg1	weight					1567:1572	the molecular weight	1553:1572	the molecular weight of LMWHs	1553:1581	The ratio of glucosamine to An-Man could be used to calculate the molecular weight of LMWHs.
30055911	4	62	theme	few	690:692	arg1	method					717:722	few practicable analytical method	690:722	few practicable analytical method	690:722	However, few practicable analytical method was available to analyze An-Man qualitatively and quantitatively.
30055911	8	63	theme	specific	1186:1193	arg1	An-Man					1204:1209	An-Man	1204:1209	An-Man	1204:1209	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	8	63	theme	specific	1186:1193	arg1	residue					1195:1201	The specific residue	1182:1201	The specific residue	1182:1201	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	9	64	theme	major	1326:1330	arg1	glucosamine					1332:1342	major glucosamine	1326:1342	major glucosamine	1326:1342	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	0	65	theme	quadrupole	161:170	arg1	time					172:175	high performance anion exchange chromatography hyphenated quadrupole time	103:175	high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	103:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	11	66	used	used	1535:1538	arg2	ratio					1495:1499	The ratio	1491:1499	The ratio of glucosamine to An-Man	1491:1524	The ratio of glucosamine to An-Man could be used to calculate the molecular weight of LMWHs.
30055911	10	67	theme	sugar	1433:1437	arg1	residues					1439:1446	these sugar residues	1427:1446	these sugar residues	1427:1446	The amounts of these sugar residues were quantitated with PAD, simultaneously.
30055911	14	68	theme	efficient	1846:1854	arg1	platform					1809:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	14	68	theme	efficient	1846:1854	arg1	way					1856:1858	an accurate, precise and efficient way	1821:1858	an accurate, precise and efficient way to identify LMWHs by determination of An-Man	1821:1903	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	12	69	theme	laser	1693:1697	arg1	scattering					1699:1708	size-exclusion chromatography-multiple angle laser scattering	1648:1708	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	0	70	theme	flight	180:185	arg1	spectrometry					192:203	flight mass spectrometry	180:203	flight mass spectrometry	180:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	3	71	theme	generated	657:665	arg1	chains					673:678	generated sugar chains	657:678	generated sugar chains	657:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	4	72	theme	analytical	706:715	arg1	method					717:722	few practicable analytical method	690:722	few practicable analytical method	690:722	However, few practicable analytical method was available to analyze An-Man qualitatively and quantitatively.
30055911	12	73	theme	angle	1687:1691	arg1	scattering					1699:1708	size-exclusion chromatography-multiple angle laser scattering	1648:1708	size-exclusion chromatography-multiple angle laser scattering detection	1648:1718	The calculated values are comparable to the value measured with size-exclusion chromatography-multiple angle laser scattering detection.
30055911	3	74	theme	reducing	635:642	arg1	terminals					644:652	the reducing terminals	631:652	the reducing terminals of generated sugar chains	631:678	Nadroparin and dalteparin are prepared through nitrous acid degradation followed by a subsequent reduction process, in which specific residue, 2,5-anhydro-d-mannitol (An-Man), is formed at the reducing terminals of generated sugar chains.
30055911	11	75	theme	LMWHs	1577:1581	arg1	weight					1567:1572	the molecular weight	1553:1572	the molecular weight of LMWHs	1553:1581	The ratio of glucosamine to An-Man could be used to calculate the molecular weight of LMWHs.
30055911	1	76	contain	have	325:328	arg2	effects					341:347	lower side effects	330:347	lower side effects	330:347	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	76	contain	have	325:328	arg1	LMWHs					237:241	LMWHs	237:241	LMWHs	237:241	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	1	76	contain	have	325:328	arg1	heparins					227:234	Low molecular weight heparins	206:234	Low molecular weight heparins (LMWHs)	206:242	Low molecular weight heparins (LMWHs), derived from unfractionated heparin (UFH) by chemical or enzymatic degradation, have lower side effects than that of heparin.
30055911	9	77	theme	minor	1348:1352	arg1	arabinose/galactose					1354:1372	minor arabinose/galactose	1348:1372	minor arabinose/galactose	1348:1372	In addition, major glucosamine and minor arabinose/galactose were observed in all heparin samples.
30055911	0	78	theme	low	68:70	arg1	heparins					89:96	low molecular weight heparins	68:96	low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	68:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	8	79	theme	nitrous	1273:1279	arg1	degradation					1286:1296	nitrous acid degradation	1273:1296	nitrous acid degradation	1273:1296	The specific residue, An-Man, was only observed in the hydrolysates of LMWHs prepared with nitrous acid degradation and reduction.
30055911	6	80	theme	Various	1037:1043	arg1	monosaccharides					1045:1059	Various monosaccharides	1037:1059	Various monosaccharides	1037:1059	An ion suppressor is set up between HPAEC and MS to remove the nonvolatile salts from HPAEC and make the elute compatible to MS. Various monosaccharides were separated well with HPAEC.
30055911	14	81	theme	precise	1834:1840	arg1	platform					1809:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	14	81	theme	precise	1834:1840	arg1	way					1856:1858	an accurate, precise and efficient way	1821:1858	an accurate, precise and efficient way to identify LMWHs by determination of An-Man	1821:1903	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	0	82	from	analysis	29:36	arg1	heparins					89:96	low molecular weight heparins	68:96	low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry	68:203	Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry.
30055911	5	83	theme	HPAEC-PAD-MS	806:817	arg1	method					819:824	a HPAEC-PAD-MS method	804:824	a HPAEC-PAD-MS method	804:824	In this work, a HPAEC-PAD-MS method was developed to analyze monosaccharides in heparin and LMWHs, especially An-Man.
30055911	14	84	theme	accurate	1824:1831	arg1	platform					1809:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform	1792:1816	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
30055911	14	84	theme	accurate	1824:1831	arg1	way					1856:1858	an accurate, precise and efficient way	1821:1858	an accurate, precise and efficient way to identify LMWHs by determination of An-Man	1821:1903	The HPAEC-PAD-MS platform is an accurate, precise and efficient way to identify LMWHs by determination of An-Man.
31129346	11	0	theme	formulations	1883:1894	arg1	profiles					1871:1878	more extended release profiles	1849:1878	more extended release profiles of formulations containing HA	1849:1908	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	12	1	theme	composite	2016:2024	arg1	films					2026:2030	composite films	2016:2030	composite films	2016:2030	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	6	2	theme	film	1151:1154	arg1	matrix					1156:1161	the film matrix	1147:1161	the film matrix	1147:1161	Amorphization of TM in the film matrix was confirmed by both DSC and XRD.
31129346	13	3	from	retention	2288:2296	arg1	surface					2312:2318	the ocular surface	2301:2318	the ocular surface	2301:2318	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	10	4	theme	film	1756:1759	arg1	surfaces					1761:1768	film surfaces	1756:1768	film surfaces	1756:1768	The SEM results confirmed smoothness and homogeneity of film surfaces for all the formulations studied.
31129346	13	5	theme	potential	2334:2342	arg1	bioavailability					2353:2367	potential improved bioavailability	2334:2367	potential improved bioavailability	2334:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	7	6	theme	ocular	1381:1386	arg1	delivery					1393:1400	controlled ocular drug delivery	1370:1400	controlled ocular drug delivery	1370:1400	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	3	7	theme	timolol	712:718	arg1	maleate					720:726	timolol maleate	712:726	timolol maleate	712:726	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	13	8	theme	excellent	2101:2109	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	8	theme	excellent	2101:2109	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	4	9	theme	physicochemical	880:894	arg1	properties					896:905	their physicochemical properties	874:905	their physicochemical properties	874:905	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	1	10	theme	ocular	220:225	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	10	theme	ocular	220:225	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	8	11	theme	mucoadhesion	1415:1426	arg1	properties					1428:1437	Tensile and mucoadhesion properties	1403:1437	Tensile and mucoadhesion properties	1403:1437	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	7	12	theme	suitable	1349:1356	arg1	polymer					1358:1364	a suitable polymer	1347:1364	HA a suitable polymer	1344:1364	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	0	13	theme	glaucoma	148:155	arg1	treatment					135:143	treatment	135:143	treatment of glaucoma	135:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	1	14	theme	delivery	232:239	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	14	theme	delivery	232:239	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	7	15	theme	HA	1344:1345	arg1	polymer					1358:1364	a suitable polymer	1347:1364	HA a suitable polymer	1344:1364	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	5	16	dep	polymer-polymer	953:967	arg1	films					983:987	composite films	973:987	composite films	973:987	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	11	17	theme	dissolution	1822:1832	arg1	studies					1834:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	13	18	used	used	2160:2163	arg2	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	18	used	used	2160:2163	arg2	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	5	19	theme	FTIR	1020:1023	arg1	results					1025:1031	FTIR results	1020:1031	FTIR results	1020:1031	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	13	20	theme	drug	2283:2286	arg1	retention					2288:2296	drug retention	2283:2296	drug retention on the ocular surface	2283:2318	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	21	theme	forming	2116:2122	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	21	theme	forming	2116:2122	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	2	22	theme	thickening	500:509	arg1	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	22	theme	thickening	500:509	arg1	agents					511:516	thickening agents	500:516	thickening agents	500:516	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	7	23	theme	release	1319:1325	arg1	profiles					1327:1334	the drug release profiles	1310:1334	the drug release profiles	1310:1334	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	12	24	theme	ocular	2055:2060	arg1	administration					2062:2075	ocular administration	2055:2075	ocular administration	2055:2075	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	3	25	theme	model	736:740	arg1	drug					751:754	model glaucoma drug	736:754	model glaucoma drug	736:754	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	7	26	theme	Swelling	1198:1205	arg1	studies					1207:1213	Swelling studies	1198:1213	Swelling studies	1198:1213	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	10	27	gly	homogeneity	1741:1751	arg1	surfaces					1761:1768	film surfaces	1756:1768	film surfaces	1756:1768	The SEM results confirmed smoothness and homogeneity of film surfaces for all the formulations studied.
31129346	13	28	theme	delivery	2254:2261	arg1	platform					2263:2270	potential topical ocular drug delivery platform	2224:2270	potential topical ocular drug delivery platform	2224:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	8	29	theme	HA	1487:1488	arg1	adhesiveness					1471:1482	adhesiveness	1471:1482	adhesiveness	1471:1482	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	8	29	theme	HA	1487:1488	arg1	elasticity					1456:1465	higher elasticity	1449:1465	higher elasticity	1449:1465	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	0	30	theme	films	93:97	arg1	characteristics					42:56	performance characteristics	30:56	performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma	30:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	1	31	theme	polymeric	176:184	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	31	theme	polymeric	176:184	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	9	32	from	effect	1530:1535	arg1	properties					1584:1593	mechanical properties	1573:1593	mechanical properties	1573:1593	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	2	33	dep	biocompatible	372:384	arg1	film					386:389	film	386:389	film	386:389	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	9	34	theme	mechanical	1573:1582	arg1	properties					1584:1593	mechanical properties	1573:1593	mechanical properties	1573:1593	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	0	35	theme	potential	102:110	arg1	platforms					121:129	potential delivery platforms	102:129	potential delivery platforms for treatment of glaucoma	102:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	5	36	theme	uniform	1073:1079	arg1	distribution					1081:1092	uniform distribution	1073:1092	uniform distribution of drug	1073:1100	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	8	37	theme	stronger	1510:1517	arg1	films					1519:1523	stronger films	1510:1523	stronger films	1510:1523	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	12	38	theme	Cytotoxicity	1911:1922	arg1	study					1924:1928	Cytotoxicity study	1911:1928	Cytotoxicity study (cell viability)	1911:1945	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	12	38	theme	Cytotoxicity	1911:1922	arg1	viability					1936:1944	cell viability	1931:1944	cell viability	1931:1944	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	7	39	theme	drug	1314:1317	arg1	profiles					1327:1334	the drug release profiles	1310:1334	the drug release profiles	1310:1334	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	6	40	from	Amorphization	1124:1136	arg1	matrix					1156:1161	the film matrix	1147:1161	the film matrix	1147:1161	Amorphization of TM in the film matrix was confirmed by both DSC and XRD.
31129346	13	41	theme	topical	2234:2240	arg1	platform					2263:2270	potential topical ocular drug delivery platform	2224:2270	potential topical ocular drug delivery platform	2224:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	5	42	theme	significant	925:935	arg1	interaction					990:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	13	43	theme	drug	2249:2252	arg1	platform					2263:2270	potential topical ocular drug delivery platform	2224:2270	potential topical ocular drug delivery platform	2224:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	3	44	theme	composite	614:622	arg1	formulations					624:635	composite formulations	614:635	composite formulations	614:635	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	4	45	theme	preliminary	763:773	arg1	optimization					775:786	preliminary optimization	763:786	preliminary optimization of transparency	763:802	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	0	46	theme	performance	30:40	arg1	characteristics					42:56	performance characteristics	30:56	performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma	30:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	0	47	theme	composite	76:84	arg1	films					93:97	timolol-loaded composite ocular films	61:97	timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma	61:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	13	48	theme	composite	2198:2206	arg1	formulations					2208:2219	composite formulations	2198:2219	composite formulations as potential topical ocular drug delivery platform	2198:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	1	49	theme	controlled	343:352	arg1	release					359:365	controlled drug release	343:365	controlled drug release	343:365	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	9	50	theme	non-sterilized	1678:1691	arg1	films					1693:1697	the sterilized and non-sterilized films	1659:1697	the sterilized and non-sterilized films	1659:1697	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	3	51	theme	casting	648:654	arg1	method					656:661	solvent casting method	640:661	solvent casting method	640:661	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	10	52	theme	SEM	1704:1706	arg1	results					1708:1714	The SEM results	1700:1714	The SEM results	1700:1714	The SEM results confirmed smoothness and homogeneity of film surfaces for all the formulations studied.
31129346	5	53	theme	composite	973:981	arg1	films					983:987	composite films	973:987	composite films	973:987	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	2	54	theme	forming	391:397	arg1	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	54	theme	forming	391:397	arg1	acid					420:423	hyaluronic acid	409:423	hyaluronic acid (HA)	409:428	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	54	theme	forming	391:397	arg1	methylcellulose					448:462	hydroxypropyl methylcellulose	434:462	hydroxypropyl methylcellulose (HPMC)	434:469	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	54	theme	forming	391:397	arg1	agents					511:516	thickening agents	500:516	thickening agents	500:516	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	9	55	theme	significant	1628:1638	arg1	difference					1640:1649	no significant difference	1625:1649	no significant difference between the sterilized and non-sterilized films	1625:1697	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	7	56	theme	swelling	1238:1245	arg1	capacity					1247:1254	remarkable swelling capacity	1227:1254	remarkable swelling capacity of HA	1227:1260	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	2	57	theme	biocompatible	372:384	arg1	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	57	theme	biocompatible	372:384	arg1	acid					420:423	hyaluronic acid	409:423	hyaluronic acid (HA)	409:428	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	57	theme	biocompatible	372:384	arg1	methylcellulose					448:462	hydroxypropyl methylcellulose	434:462	hydroxypropyl methylcellulose (HPMC)	434:469	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	57	theme	biocompatible	372:384	arg1	agents					511:516	thickening agents	500:516	thickening agents	500:516	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	11	58	dep	in	1808:1809	arg1	vitro					1811:1815	vitro	1811:1815	vitro	1811:1815	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	4	59	theme	handling	816:823	arg1	ease					808:811	ease	808:811	ease of handling	808:823	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	4	59	theme	handling	816:823	arg1	optimization					775:786	preliminary optimization	763:786	preliminary optimization of transparency	763:802	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	12	60	theme	single	1997:2002	arg1	polymer					2004:2010	the single polymer	1993:2010	the single polymer	1993:2010	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	12	60	theme	single	1997:2002	arg1	safe					2046:2049	safe	2046:2049	safe	2046:2049	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	9	61	theme	sterilized	1663:1672	arg1	films					1693:1697	the sterilized and non-sterilized films	1659:1697	the sterilized and non-sterilized films	1659:1697	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	10	62	theme	surfaces	1761:1768	arg1	homogeneity					1741:1751	homogeneity	1741:1751	homogeneity	1741:1751	The SEM results confirmed smoothness and homogeneity of film surfaces for all the formulations studied.
31129346	10	62	theme	surfaces	1761:1768	arg1	smoothness					1726:1735	smoothness	1726:1735	smoothness	1726:1735	The SEM results confirmed smoothness and homogeneity of film surfaces for all the formulations studied.
31129346	4	63	theme	transparency	791:802	arg1	ease					808:811	ease	808:811	ease of handling	808:823	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	4	63	theme	transparency	791:802	arg1	optimization					775:786	preliminary optimization	763:786	preliminary optimization of transparency	763:802	After preliminary optimization of transparency and ease of handling, the formulations were further characterized for their physicochemical properties.
31129346	13	64	theme	improved	2344:2351	arg1	bioavailability					2353:2367	potential improved bioavailability	2334:2367	potential improved bioavailability	2334:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	7	65	theme	drug	1388:1391	arg1	delivery					1393:1400	controlled ocular drug delivery	1370:1400	controlled ocular drug delivery	1370:1400	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	11	66	theme	drug	1817:1820	arg1	studies					1834:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	7	67	with	comparison	1265:1274	arg1	HPMC					1281:1284	HPMC	1281:1284	HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery	1281:1400	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	3	68	theme	different	550:558	arg1	films					560:564	Two different films	546:564	Two different films	546:564	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	3	68	theme	different	550:558	arg1	polymer					594:600	single polymer	587:600	single polymer	587:600	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	1	69	theme	potential	210:218	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	69	theme	potential	210:218	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	6	70	theme	TM	1141:1142	arg1	Amorphization					1124:1136	Amorphization	1124:1136	Amorphization of TM in the film matrix	1124:1161	Amorphization of TM in the film matrix was confirmed by both DSC and XRD.
31129346	3	71	dep	prepared	571:578	arg1	i					581:581	i	581:581	i	581:581	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	11	72	theme	in	1808:1809	arg1	studies					1834:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies	1804:1840	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	1	73	from	retention	266:274	arg1	eye					283:285	the eye	279:285	the eye	279:285	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	5	74	theme	IR	1053:1054	arg1	mapping					1056:1062	IR mapping	1053:1062	IR mapping	1053:1062	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	1	75	theme	drug	227:230	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	75	theme	drug	227:230	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	8	76	theme	higher	1449:1454	arg1	elasticity					1456:1465	higher elasticity	1449:1465	higher elasticity	1449:1465	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	13	77	theme	HPMC	2142:2145	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	77	theme	HPMC	2142:2145	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	12	78	theme	HeLa	1966:1969	arg1	cells					1971:1975	HeLa cells	1966:1975	HeLa cells	1966:1975	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	2	79	used	used	492:495	arg2	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	79	used	used	492:495	arg2	acid					420:423	hyaluronic acid	409:423	hyaluronic acid (HA)	409:428	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	79	used	used	492:495	arg2	agents					511:516	thickening agents	500:516	thickening agents	500:516	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	79	used	used	492:495	arg2	methylcellulose					448:462	hydroxypropyl methylcellulose	434:462	hydroxypropyl methylcellulose (HPMC)	434:469	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	13	80	theme	film	2111:2114	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	80	theme	film	2111:2114	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	11	81	theme	extended	1854:1861	arg1	profiles					1871:1878	more extended release profiles	1849:1878	more extended release profiles of formulations containing HA	1849:1908	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	0	82	theme	ocular	86:91	arg1	films					93:97	timolol-loaded composite ocular films	61:97	timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma	61:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	3	83	theme	single	587:592	arg1	polymer					594:600	single polymer	587:600	single polymer	587:600	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	3	83	theme	single	587:592	arg1	films					560:564	Two different films	546:564	Two different films	546:564	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	1	84	theme	Thin	158:161	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	84	theme	Thin	158:161	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	3	85	theme	glaucoma	742:749	arg1	drug					751:754	model glaucoma drug	736:754	model glaucoma drug	736:754	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	1	86	theme	drug	354:357	arg1	release					359:365	controlled drug release	343:365	controlled drug release	343:365	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	87	theme	erodible	167:174	arg1	films					186:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films	158:190	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	1	87	theme	erodible	167:174	arg1	systems					241:247	potential ocular drug delivery systems	210:247	potential ocular drug delivery systems	210:247	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31129346	0	88	theme	delivery	112:119	arg1	platforms					121:129	potential delivery platforms	102:129	potential delivery platforms for treatment of glaucoma	102:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	12	89	theme	MTT	1953:1955	arg1	assay					1957:1961	MTT assay	1953:1961	MTT assay on HeLa cells	1953:1975	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	5	90	theme	drug-polymer	937:948	arg1	interaction					990:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	7	91	theme	controlled	1370:1379	arg1	delivery					1393:1400	controlled ocular drug delivery	1370:1400	controlled ocular drug delivery	1370:1400	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	2	92	theme	eye	521:523	arg1	drops					525:529	eye drops	521:529	eye drops	521:529	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	13	93	theme	ocular	2305:2310	arg1	surface					2312:2318	the ocular surface	2301:2318	the ocular surface	2301:2318	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	5	94	theme	polymer-polymer	953:967	arg1	interaction					990:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	significant drug-polymer or polymer-polymer (in composite films) interaction	925:1000	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	2	95	theme	hyaluronic	409:418	arg1	HA					426:427	HA	426:427	HA	426:427	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	95	theme	hyaluronic	409:418	arg1	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	95	theme	hyaluronic	409:418	arg1	acid					420:423	hyaluronic acid	409:423	hyaluronic acid (HA)	409:428	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	5	96	theme	drug	1097:1100	arg1	distribution					1081:1092	uniform distribution	1073:1092	uniform distribution of drug	1073:1100	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	12	97	theme	cell	1931:1934	arg1	study					1924:1928	Cytotoxicity study	1911:1928	Cytotoxicity study (cell viability)	1911:1945	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	12	97	theme	cell	1931:1934	arg1	viability					1936:1944	cell viability	1931:1944	cell viability	1931:1944	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	9	98	theme	UV	1557:1558	arg1	radiation					1560:1568	UV radiation	1557:1568	UV radiation	1557:1568	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	13	99	theme	HA	2135:2136	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	99	theme	HA	2135:2136	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	100	theme	potential	2224:2232	arg1	platform					2263:2270	potential topical ocular drug delivery platform	2224:2270	potential topical ocular drug delivery platform	2224:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	11	101	contain	containing	1896:1905	arg2	HA					1907:1908	HA	1907:1908	HA	1907:1908	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	11	101	contain	containing	1896:1905	arg1	formulations					1883:1894	formulations	1883:1894	formulations containing HA	1883:1908	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	2	102	theme	hydroxypropyl	434:446	arg1	polymers					399:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers	368:406	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	102	theme	hydroxypropyl	434:446	arg1	HPMC					465:468	HPMC	465:468	HPMC	465:468	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	2	102	theme	hydroxypropyl	434:446	arg1	methylcellulose					448:462	hydroxypropyl methylcellulose	434:462	hydroxypropyl methylcellulose (HPMC)	434:469	Two biocompatible film forming polymers, hyaluronic acid (HA) and hydroxypropyl methylcellulose (HPMC), which are currently used as thickening agents in eye drops were employed.
31129346	9	103	theme	sterilization	1540:1552	arg1	effect					1530:1535	The effect	1526:1535	The effect of sterilization by UV radiation on mechanical properties	1526:1593	The effect of sterilization by UV radiation on mechanical properties was also evaluated and showed no significant difference between the sterilized and non-sterilized films.
31129346	0	104	theme	characteristics	42:56	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	0	104	theme	characteristics	42:56	arg1	Development					0:10	Development	0:10	Development	0:10	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	13	105	theme	ocular	2242:2247	arg1	platform					2263:2270	potential topical ocular drug delivery platform	2224:2270	potential topical ocular drug delivery platform	2224:2270	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	5	106	theme	interaction	990:1000	arg1	indication					911:920	No indication	908:920	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction	908:1000	No indication of significant drug-polymer or polymer-polymer (in composite films) interaction was observed from FTIR results while evaluation by IR mapping revealed uniform distribution of drug throughout the films.
31129346	0	107	theme	timolol-loaded	61:74	arg1	films					93:97	timolol-loaded composite ocular films	61:97	timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma	61:155	Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma.
31129346	7	108	theme	HA	1259:1260	arg1	capacity					1247:1254	remarkable swelling capacity	1227:1254	remarkable swelling capacity of HA	1227:1260	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	3	109	theme	solvent	640:646	arg1	method					656:661	solvent casting method	640:661	solvent casting method	640:661	Two different films were prepared (i) as single polymer and (ii) as composite formulations by solvent casting method, incorporating glycerol (GLY) as plasticizer and timolol maleate (TM) as model glaucoma drug.
31129346	13	110	theme	present	2082:2088	arg1	work					2090:2093	The present work	2078:2093	The present work	2078:2093	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	11	111	theme	release	1863:1869	arg1	profiles					1871:1878	more extended release profiles	1849:1878	more extended release profiles of formulations containing HA	1849:1908	The in vitro drug dissolution studies showed more extended release profiles of formulations containing HA.
31129346	13	112	theme	single	2168:2173	arg1	ability					2124:2130	excellent film forming ability	2101:2130	excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability	2101:2367	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	13	112	theme	single	2168:2173	arg1	polymer					2175:2181	single polymer	2168:2181	single polymer	2168:2181	The present work shows excellent film forming ability of HA and HPMC which can be used as single polymer or combined in composite formulations as potential topical ocular drug delivery platform to enhance drug retention on the ocular surface and therefore potential improved bioavailability.
31129346	12	113	from	assay	1957:1961	arg1	cells					1971:1975	HeLa cells	1966:1975	HeLa cells	1966:1975	Cytotoxicity study (cell viability) using MTT assay on HeLa cells, confirmed that the single polymer and composite films are generally safe for ocular administration.
31129346	7	114	theme	remarkable	1227:1236	arg1	capacity					1247:1254	remarkable swelling capacity	1227:1254	remarkable swelling capacity of HA	1227:1260	Swelling studies illustrated remarkable swelling capacity of HA in comparison with HPMC which directly affected the drug release profiles, making HA a suitable polymer for controlled ocular drug delivery.
31129346	8	115	theme	Tensile	1403:1409	arg1	properties					1428:1437	Tensile and mucoadhesion properties	1403:1437	Tensile and mucoadhesion properties	1403:1437	Tensile and mucoadhesion properties confirmed higher elasticity and adhesiveness of HA while HPMC produced stronger films.
31129346	1	116	theme	drug	261:264	arg1	retention					266:274	drug retention	261:274	drug retention on the eye	261:285	Thin and erodible polymeric films were developed as potential ocular drug delivery systems to increase drug retention on the eye with the aim of improving bioavailability and achieving controlled drug release.
31888856	3	0	theme	rod-like	799:806	arg1	structure					822:830	a rod-like to sheet-like structure	797:830	a rod-like to sheet-like structure	797:830	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	4	1	theme	α-glucosidase	936:948	arg1	adsorption					977:986	the adsorption	973:986	the adsorption of cholesterol	973:1001	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	4	1	theme	α-glucosidase	936:948	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	5	2	dep	in	1098:1099	arg1	vitro					1101:1105	vitro	1101:1105	vitro	1101:1105	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	2	3	theme	2D-NMR	364:369	arg1	analyses					371:378	2D-NMR analyses	364:378	2D-NMR analyses	364:378	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	5	4	theme	barley	1135:1140	arg1	β-glucan					1142:1149	barley β-glucan	1135:1149	barley β-glucan	1135:1149	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	0	5	from	Effects	0:6	arg1	activities					81:90	in vitro physiological activities	58:90	in vitro physiological activities of barley	58:100	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	0	5	from	Effects	0:6	arg1	characteristics					38:52	structural characteristics	27:52	structural characteristics	27:52	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	5	6	theme	lipid	1186:1190	arg1	metabolism					1192:1201	lipid metabolism	1186:1201	lipid metabolism	1186:1201	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	3	7	dep	D	643:643	arg1	to					631:632	to	631:632	to	631:632	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	0	8	theme	barley	95:100	arg1	activities					81:90	in vitro physiological activities	58:90	in vitro physiological activities of barley	58:100	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	0	8	theme	barley	95:100	arg1	characteristics					38:52	structural characteristics	27:52	structural characteristics	27:52	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	3	9	dep	features	769:776	arg1	transforming					779:790	transforming	779:790	transforming from a rod-like to sheet-like structure	779:830	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	2	10	theme	infrared	293:300	arg1	spectroscopy					302:313	infrared spectroscopy	293:313	infrared spectroscopy	293:313	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	1	11	theme	barley	206:211	arg1	β-glucan					213:220	barley β-glucan	206:220	barley β-glucan	206:220	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	5	12	theme	related	1163:1169	arg1	β-glucan					1142:1149	barley β-glucan	1135:1149	barley β-glucan	1135:1149	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	1	13	theme	main	179:182	arg1	changes					195:201	the main structural changes	175:201	the main structural changes of barley β-glucan and their in vitro activities	175:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	4	14	theme	lipase	954:959	arg1	adsorption					977:986	the adsorption	973:986	the adsorption of cholesterol	973:1001	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	4	14	theme	lipase	954:959	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	15	theme	β-	664:665	arg1	residues					672:679	the β-(1→3) residues	660:679	the β-(1→3) residues	660:679	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	4	16	theme	improved	891:898	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	17	theme	sheet-like	811:820	arg1	structure					822:830	a rod-like to sheet-like structure	797:830	a rod-like to sheet-like structure	797:830	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	1	18	theme	structural	184:193	arg1	changes					195:201	the main structural changes	175:201	the main structural changes of barley β-glucan and their in vitro activities	175:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	5	19	theme	in	1098:1099	arg1	activities					1121:1130	in vitro physiological activities	1098:1130	in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism	1098:1201	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	1	20	from	effects	116:122	arg1	changes					195:201	the main structural changes	175:201	the main structural changes of barley β-glucan and their in vitro activities	175:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	2	21	theme	electron	393:400	arg1	microscopy					402:411	scanning electron microscopy	384:411	scanning electron microscopy	384:411	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	3	22	from	structure	822:830	arg1	transforming					779:790	transforming	779:790	transforming from a rod-like to sheet-like structure	779:830	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	2	23	theme	β-	543:544	arg1	glucose					558:564	β-(1→3) and β-(1→4) linked glucose	531:564	β-(1→3) and β-(1→4) linked glucose	531:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	24	theme	monosaccharide	316:329	arg1	composition					331:341	monosaccharide composition	316:341	monosaccharide composition	316:341	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	25	theme	barley	437:442	arg1	RBG					454:456	(raw barley β-glucan) RBG	432:456	(raw barley β-glucan) RBG	432:456	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	25	theme	barley	437:442	arg1	β-glucan					444:451	raw barley β-glucan	433:451	raw barley β-glucan	433:451	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	0	26	theme	fermentation	11:22	arg1	Effects					0:6	Effects	0:6	Effects of fermentation on structural characteristics and in vitro physiological activities of barley	0:100	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	5	27	theme	physiological	1107:1119	arg1	activities					1121:1130	in vitro physiological activities	1098:1130	in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism	1098:1201	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	2	28	theme	scanning	384:391	arg1	microscopy					402:411	scanning electron microscopy	384:411	scanning electron microscopy	384:411	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	29	link	linked	551:556	arg1	glucose					558:564	β-(1→3) and β-(1→4) linked glucose	531:564	β-(1→3) and β-(1→4) linked glucose	531:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	30	theme	raw	433:435	arg1	RBG					454:456	(raw barley β-glucan) RBG	432:456	(raw barley β-glucan) RBG	432:456	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	30	theme	raw	433:435	arg1	β-glucan					444:451	raw barley β-glucan	433:451	raw barley β-glucan	433:451	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	4	31	theme	α-amylase	925:933	arg1	adsorption					977:986	the adsorption	973:986	the adsorption of cholesterol	973:1001	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	4	31	theme	α-amylase	925:933	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	32	theme	residues	672:679	arg1	features					769:776	microstructure features	754:776	microstructure features (transforming from a rod-like to sheet-like structure)	754:831	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	3	32	theme	residues	672:679	arg1	ratio					651:655	the ratio	647:655	the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24)	647:748	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	3	32	theme	residues	672:679	arg1	weight					596:601	different molecular weight	576:601	different molecular weight (decreasing from 1.13×105 D to 6.35×104 D)	576:644	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	4	33	theme	Bioassay	848:855	arg1	results					857:863	Bioassay results	848:863	Bioassay results	848:863	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	34	theme	D	629:629	arg1	D					643:643	1.13×105 D to 6.35×104 D	620:643	1.13×105 D to 6.35×104 D	620:643	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	0	35	dep	in	58:59	arg1	vitro					61:65	vitro	61:65	vitro	61:65	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	1	36	theme	β-glucan	213:220	arg1	changes					195:201	the main structural changes	175:201	the main structural changes of barley β-glucan and their in vitro activities	175:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	0	37	theme	structural	27:36	arg1	characteristics					38:52	structural characteristics	27:52	structural characteristics	27:52	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	3	38	theme	1→4	691:693	arg1	residues					696:703	the β-(1→4) residues	684:703	the β-(1→4) residues	684:703	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	0	39	theme	in	58:59	arg1	activities					81:90	in vitro physiological activities	58:90	in vitro physiological activities of barley	58:100	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	2	40	theme	linked	551:556	arg1	glucose					558:564	β-(1→3) and β-(1→4) linked glucose	531:564	β-(1→3) and β-(1→4) linked glucose	531:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	1	41	dep	in	232:233	arg1	vitro					235:239	vitro	235:239	vitro	235:239	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	2	42	theme	fermented	462:470	arg1	RBG					454:456	(raw barley β-glucan) RBG	432:456	(raw barley β-glucan) RBG	432:456	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	42	theme	fermented	462:470	arg1	FBG					489:491	FBG	489:491	FBG	489:491	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	42	theme	fermented	462:470	arg1	polysaccharides					498:512	polysaccharides	498:512	polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose	498:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	42	theme	fermented	462:470	arg1	β-glucan					479:486	fermented barley β-glucan	462:486	fermented barley β-glucan (FBG)	462:492	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	3	43	theme	β-	688:689	arg1	residues					696:703	the β-(1→4) residues	684:703	the β-(1→4) residues	684:703	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	3	44	dep	structure	822:830	arg1	to					808:809	to	808:809	to	808:809	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	4	45	theme	cholesterol	991:1001	arg1	adsorption					977:986	the adsorption	973:986	the adsorption of cholesterol	973:1001	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	4	45	theme	cholesterol	991:1001	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	46	theme	molecular	586:594	arg1	weight					596:601	different molecular weight	576:601	different molecular weight (decreasing from 1.13×105 D to 6.35×104 D)	576:644	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	2	47	theme	Molecular	266:274	arg1	characteristics					276:290	Molecular characteristics	266:290	Molecular characteristics	266:290	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	1	48	theme	fermentation	127:138	arg1	effects					116:122	The effects	112:122	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities	112:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	4	49	theme	inhibitory	900:909	arg1	activities					911:920	improved inhibitory activities	891:920	improved inhibitory activities of α-amylase, α-glucosidase and lipase	891:959	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	3	50	dep	1:1.8-1:2.24	736:747	arg1	to					733:734	to	733:734	to	733:734	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	4	51	theme	acidic	1009:1014	arg1	conditions					1016:1025	acidic conditions	1009:1025	acidic conditions	1009:1025	Bioassay results showed that FBG exhibited improved inhibitory activities of α-amylase, α-glucosidase and lipase, as well as the adsorption of cholesterol under acidic conditions compared to RBG.
31888856	1	52	theme	in	232:233	arg1	activities					241:250	their in vitro activities	226:250	their in vitro activities	226:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	0	53	theme	physiological	67:79	arg1	activities					81:90	in vitro physiological activities	58:90	in vitro physiological activities of barley	58:100	Effects of fermentation on structural characteristics and in vitro physiological activities of barley β-glucan.
31888856	3	54	theme	different	576:584	arg1	weight					596:601	different molecular weight	576:601	different molecular weight (decreasing from 1.13×105 D to 6.35×104 D)	576:644	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	1	55	theme	Lactobacillus	143:155	arg1	dy-1					167:170	Lactobacillus plantarum dy-1	143:170	Lactobacillus plantarum dy-1	143:170	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	3	56	theme	microstructure	754:767	arg1	features					769:776	microstructure features	754:776	microstructure features (transforming from a rod-like to sheet-like structure)	754:831	However, different molecular weight (decreasing from 1.13×105 D to 6.35×104 D), the ratio of the β-(1→3) residues to the β-(1→4) residues (ranging from 1:1.98-1:2.50 to 1:1.8-1:2.24) and microstructure features (transforming from a rod-like to sheet-like structure) were observed.
31888856	2	57	theme	barley	472:477	arg1	RBG					454:456	(raw barley β-glucan) RBG	432:456	(raw barley β-glucan) RBG	432:456	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	57	theme	barley	472:477	arg1	FBG					489:491	FBG	489:491	FBG	489:491	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	57	theme	barley	472:477	arg1	polysaccharides					498:512	polysaccharides	498:512	polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose	498:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	2	57	theme	barley	472:477	arg1	β-glucan					479:486	fermented barley β-glucan	462:486	fermented barley β-glucan (FBG)	462:492	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
31888856	1	58	theme	activities	241:250	arg1	changes					195:201	the main structural changes	175:201	the main structural changes of barley β-glucan and their in vitro activities	175:250	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	5	59	theme	β-glucan	1142:1149	arg1	activities					1121:1130	in vitro physiological activities	1098:1130	in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism	1098:1201	These results suggested that fermentation may enhance in vitro physiological activities of barley β-glucan, especially related to glucose and lipid metabolism.
31888856	1	60	theme	plantarum	157:165	arg1	dy-1					167:170	Lactobacillus plantarum dy-1	143:170	Lactobacillus plantarum dy-1	143:170	The effects of fermentation by Lactobacillus plantarum dy-1 on the main structural changes of barley β-glucan and their in vitro activities were studied.
31888856	2	61	theme	β-	531:532	arg1	glucose					558:564	β-(1→3) and β-(1→4) linked glucose	531:564	β-(1→3) and β-(1→4) linked glucose	531:564	Molecular characteristics, infrared spectroscopy, monosaccharide composition, methylation, 1D and 2D-NMR analyses and scanning electron microscopy revealed that both (raw barley β-glucan) RBG and fermented barley β-glucan (FBG) are polysaccharides predominanted by β-(1→3) and β-(1→4) linked glucose.
30974193	0	0	theme	shell	89:93	arg1	materials					95:103	various shell materials	81:103	various shell materials: Improvements and mechanism analysis	81:140	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	2	1	theme	hydroxypropyl	536:548	arg1	HPMC					567:570	HPMC	567:570	HPMC	567:570	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	1	theme	hydroxypropyl	536:548	arg1	methylcellulose					550:564	hydroxypropyl methylcellulose	536:564	hydroxypropyl methylcellulose (HPMC)	536:571	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	5	2	theme	qualified	1206:1214	arg1	whole					1150:1154	whole	1150:1154	whole	1150:1154	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	5	2	theme	qualified	1206:1214	arg1	tablets					1216:1222	qualified tablets	1206:1222	qualified tablets with high ZR loadings	1206:1244	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	4	3	theme	simple	811:816	arg1	process					818:824	a simple process	809:824	a simple process of superposition and transmission of the physical properties of raw materials	809:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	3	theme	simple	811:816	arg1	coating					793:799	(i) fluid bed coating	779:799	(i) fluid bed coating	779:799	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	0	4	theme	various	81:87	arg1	materials					95:103	various shell materials	81:103	various shell materials: Improvements and mechanism analysis	81:140	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	2	5	dep	powder	411:416	arg1	powder					448:453	a natural plant product powder	424:453	a natural plant product powder with poor DC properties	424:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	4	6	theme	DC	964:965	arg1	properties					967:976	the DC properties	960:976	the DC properties	960:976	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	2	7	theme	poor	460:463	arg1	properties					468:477	poor DC properties	460:477	poor DC properties	460:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	5	8	theme	continuous	1277:1286	arg1	DC					1288:1289	continuous DC	1277:1289	continuous DC in this study	1277:1303	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	1	9	theme	natural	253:259	arg1	tablets					281:287	natural plant product (NPP) tablets	253:287	natural plant product (NPP) tablets	253:287	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	5	10	theme	core-shell	1184:1193	arg1	particles					1195:1203	core-shell particles	1184:1203	core-shell particles	1184:1203	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	5	11	with	tablets	1216:1222	arg1	loadings					1237:1244	high ZR loadings	1229:1244	high ZR loadings	1229:1244	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	1	12	theme	plant	261:265	arg1	tablets					281:287	natural plant product (NPP) tablets	253:287	natural plant product (NPP) tablets	253:287	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	6	13	theme	tablets	1381:1387	arg1	development					1352:1362	the development	1348:1362	the development of natural plant tablets	1348:1387	These findings are beneficial to boosting the development of natural plant tablets through DC.
30974193	4	14	theme	superposition	829:841	arg1	process					818:824	a simple process	809:824	a simple process of superposition and transmission of the physical properties of raw materials	809:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	14	theme	superposition	829:841	arg1	coating					793:799	(i) fluid bed coating	779:799	(i) fluid bed coating	779:799	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	2	15	theme	product	440:446	arg1	powder					448:453	a natural plant product powder	424:453	a natural plant product powder with poor DC properties	424:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	16	with	powder	448:453	arg1	properties					468:477	poor DC properties	460:477	poor DC properties	460:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	0	17	dep	materials	95:103	arg1	Improvements					106:117	Improvements	106:117	Improvements	106:117	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	0	17	dep	materials	95:103	arg1	analysis					133:140	mechanism analysis	123:140	mechanism analysis	123:140	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	5	18	theme	design	1174:1179	arg1	virtue					1160:1165	virtue	1160:1165	virtue of the design of core-shell particles	1160:1203	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	1	19	theme	more	175:178	arg1	attention					189:197	more and more attention	175:197	more and more attention	175:197	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	0	20	theme	compaction	7:16	arg1	properties					18:27	Direct compaction properties	0:27	Direct compaction properties of Zingiberis Rhizoma	0:49	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	4	21	theme	fluid	783:787	arg1	process					818:824	a simple process	809:824	a simple process of superposition and transmission of the physical properties of raw materials	809:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	21	theme	fluid	783:787	arg1	coating					793:799	(i) fluid bed coating	779:799	(i) fluid bed coating	779:799	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	22	theme	materials	894:902	arg1	properties					876:885	the physical properties	863:885	the physical properties of raw materials	863:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	6	23	theme	natural	1367:1373	arg1	tablets					1381:1387	natural plant tablets	1367:1387	natural plant tablets	1367:1387	These findings are beneficial to boosting the development of natural plant tablets through DC.
30974193	2	24	theme	composite	343:351	arg1	particles					353:361	composite particles	343:361	composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties)	343:478	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	24	theme	composite	343:351	arg1	CPs					364:366	CPs	364:366	CPs	364:366	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	0	25	theme	Direct	0:5	arg1	properties					18:27	Direct compaction properties	0:27	Direct compaction properties of Zingiberis Rhizoma	0:49	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	1	26	theme	product	267:273	arg1	tablets					281:287	natural plant product (NPP) tablets	253:287	natural plant product (NPP) tablets	253:287	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	1	27	theme	more	184:187	arg1	attention					189:197	more and more attention	175:197	more and more attention	175:197	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	3	28	theme	physical	667:674	arg1	properties					676:685	Their physical properties	661:685	Their physical properties	661:685	Their physical properties and compacting parameters were characterized comprehensively.
30974193	4	29	theme	bed	789:791	arg1	process					818:824	a simple process	809:824	a simple process of superposition and transmission of the physical properties of raw materials	809:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	29	theme	bed	789:791	arg1	coating					793:799	(i) fluid bed coating	779:799	(i) fluid bed coating	779:799	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	5	30	theme	particles	1195:1203	arg1	design					1174:1179	the design	1170:1179	the design of core-shell particles	1170:1203	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	1	31	theme	NPP	276:278	arg1	tablets					281:287	natural plant product (NPP) tablets	253:287	natural plant product (NPP) tablets	253:287	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	0	32	theme	mechanism	123:131	arg1	analysis					133:140	mechanism analysis	123:140	mechanism analysis	123:140	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	0	33	theme	Rhizoma	43:49	arg1	properties					18:27	Direct compaction properties	0:27	Direct compaction properties of Zingiberis Rhizoma	0:49	Direct compaction properties of Zingiberis Rhizoma extracted powders coated with various shell materials: Improvements and mechanism analysis.
30974193	1	34	from	application	238:248	arg1	tablets					281:287	natural plant product (NPP) tablets	253:287	natural plant product (NPP) tablets	253:287	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	4	35	theme	ZR	1141:1142	arg1	flowability					1126:1136	flowability	1126:1136	flowability	1126:1136	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	35	theme	ZR	1141:1142	arg1	compactibility					1107:1120	compactibility	1107:1120	compactibility	1107:1120	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	36	theme	problematic	981:991	arg1	best					1073:1076	best	1073:1076	best	1073:1076	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	36	theme	problematic	981:991	arg1	combination					1019:1029	the combination	1015:1029	the combination of 7% HPMC and 1% silica	1015:1054	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	36	theme	problematic	981:991	arg1	ZR					993:994	problematic ZR	981:994	problematic ZR to some degree	981:1009	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	1	37	theme	tablet	203:208	arg1	manufacturing					210:222	tablet manufacturing	203:222	tablet manufacturing	203:222	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	3	38	theme	compacting	691:700	arg1	parameters					702:711	compacting parameters	691:711	compacting parameters	691:711	Their physical properties and compacting parameters were characterized comprehensively.
30974193	2	39	theme	DC	465:466	arg1	properties					468:477	poor DC properties	460:477	poor DC properties	460:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	4	40	theme	silica	1049:1054	arg1	best					1073:1076	best	1073:1076	best	1073:1076	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	40	theme	silica	1049:1054	arg1	combination					1019:1029	the combination	1015:1029	the combination of 7% HPMC and 1% silica	1015:1054	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	40	theme	silica	1049:1054	arg1	ZR					993:994	problematic ZR	981:994	problematic ZR to some degree	981:1009	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	41	theme	properties	876:885	arg1	transmission					847:858	transmission	847:858	transmission	847:858	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	41	theme	properties	876:885	arg1	superposition					829:841	superposition	829:841	superposition	829:841	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	42	dep	coating	793:799	arg1	i					780:780	i	780:780	i	780:780	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	43	theme	physical	867:874	arg1	properties					876:885	the physical properties	863:885	the physical properties of raw materials	863:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	2	44	theme	shell	509:513	arg1	polyvinylpyrrolidone					574:593	polyvinylpyrrolidone	574:593	polyvinylpyrrolidone (PVP)	574:599	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	inulin					611:616	inulin	611:616	inulin	611:616	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	methylcellulose					550:564	hydroxypropyl methylcellulose	536:564	hydroxypropyl methylcellulose (HPMC)	536:571	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	mannitol					619:626	mannitol	619:626	mannitol	619:626	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	combinations					647:658	their combinations	641:658	their combinations	641:658	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	materials					515:523	different shell materials	499:523	different shell materials	499:523	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	dextran					602:608	dextran	602:608	dextran	602:608	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	44	theme	shell	509:513	arg1	silica					629:634	silica	629:634	silica	629:634	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	4	45	theme	raw	890:892	arg1	materials					894:902	raw materials	890:902	raw materials	890:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	46	theme	%	1047:1047	arg1	silica					1049:1054	1% silica	1046:1054	1% silica	1046:1054	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	47	theme	transmission	847:858	arg1	process					818:824	a simple process	809:824	a simple process of superposition and transmission of the physical properties of raw materials	809:902	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	47	theme	transmission	847:858	arg1	coating					793:799	(i) fluid bed coating	779:799	(i) fluid bed coating	779:799	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	2	48	theme	different	499:507	arg1	polyvinylpyrrolidone					574:593	polyvinylpyrrolidone	574:593	polyvinylpyrrolidone (PVP)	574:599	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	inulin					611:616	inulin	611:616	inulin	611:616	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	methylcellulose					550:564	hydroxypropyl methylcellulose	536:564	hydroxypropyl methylcellulose (HPMC)	536:571	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	mannitol					619:626	mannitol	619:626	mannitol	619:626	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	combinations					647:658	their combinations	641:658	their combinations	641:658	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	materials					515:523	different shell materials	499:523	different shell materials	499:523	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	dextran					602:608	dextran	602:608	dextran	602:608	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	48	theme	different	499:507	arg1	silica					629:634	silica	629:634	silica	629:634	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	5	49	theme	high	1229:1232	arg1	loadings					1237:1244	high ZR loadings	1229:1244	high ZR loadings	1229:1244	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	5	50	theme	ZR	1234:1235	arg1	loadings					1237:1244	high ZR loadings	1229:1244	high ZR loadings	1229:1244	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	4	51	theme	HPMC	1037:1040	arg1	%					1035:1035	7% HPMC	1034:1040	7% HPMC	1034:1040	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	1	52	theme	Direct	143:148	arg1	DC					162:163	DC	162:163	DC	162:163	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	1	52	theme	Direct	143:148	arg1	compaction					150:159	Direct compaction	143:159	Direct compaction (DC)	143:164	Direct compaction (DC) attracts more and more attention for tablet manufacturing; however, its application in natural plant product (NPP) tablets is still extremely limited.
30974193	6	53	theme	plant	1375:1379	arg1	tablets					1381:1387	natural plant tablets	1367:1387	natural plant tablets	1367:1387	These findings are beneficial to boosting the development of natural plant tablets through DC.
30974193	2	54	theme	plant	434:438	arg1	powder					448:453	a natural plant product powder	424:453	a natural plant product powder with poor DC properties	424:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	4	55	theme	%	1035:1035	arg1	best					1073:1076	best	1073:1076	best	1073:1076	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	55	theme	%	1035:1035	arg1	combination					1019:1029	the combination	1015:1029	the combination of 7% HPMC and 1% silica	1015:1054	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	55	theme	%	1035:1035	arg1	ZR					993:994	problematic ZR	981:994	problematic ZR to some degree	981:1009	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	5	56	from	DC	1288:1289	arg1	study					1299:1303	this study	1294:1303	this study	1294:1303	As a whole, by virtue of the design of core-shell particles, qualified tablets with high ZR loadings were successfully produced via continuous DC in this study.
30974193	2	57	theme	particles	353:361	arg1	kinds					334:338	8 kinds	332:338	8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties)	332:478	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	2	58	theme	natural	426:432	arg1	powder					448:453	a natural plant product powder	424:453	a natural plant product powder with poor DC properties	424:477	In this study, 8 kinds of composite particles (CPs) based on the Zingiberis Rhizoma extracted powder (ZR) (a natural plant product powder with poor DC properties) were prepared with different shell materials, including hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), dextran, inulin, mannitol, silica, and their combinations.
30974193	4	59	dep	process	818:824	arg1	studied					938:944	studied	938:944	studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR	938:1142	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	60	theme	1	1046:1046	arg1	%					1047:1047	%	1047:1047	%	1047:1047	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	61	theme	shell	922:926	arg1	materials					928:936	all the shell materials	914:936	all the shell materials	914:936	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
30974193	4	62	dep	compactibility	1107:1120	arg1	the					1103:1105	the	1103:1105	the	1103:1105	The results demonstrated that (i) fluid bed coating was not a simple process of superposition and transmission of the physical properties of raw materials; and (ii) all the shell materials studied could improve the DC properties of problematic ZR to some degree and the combination of 7% HPMC and 1% silica showed to be the best to markedly improve both the compactibility and flowability of ZR.
31210628	4	0	theme	sucrose	896:902	arg1	dextransucrases					863:877	the dextransucrases	859:877	the dextransucrases independently of sucrose	859:902	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	10	1	theme	L.	1967:1968	arg1	TMW					1978:1980	L. nagelii TMW 1.1827	1967:1987	L. nagelii TMW 1.1827	1967:1987	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	6	2	theme	weight	1204:1209	arg1	terms					1161:1165	terms	1161:1165	terms of molecular structure and molecular weight	1161:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	3	3	theme	C-terminal	579:588	arg1	domain					605:610	an additional C-terminal glucan-binding domain	565:610	an additional C-terminal glucan-binding domain	565:610	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	3	theme	C-terminal	579:588	arg1	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	11	4	theme	water	2078:2082	arg1	borne					2090:2094	water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827	2078:2141	borne	2090:2094	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	10	5	gly	glycosylation	1859:1871	arg1	glucansucrases					1876:1889	glucansucrases	1876:1889	glucansucrases	1876:1889	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	9	6	theme	Da	1749:1750	arg1	Mw					1734:1735	Mw	1734:1735	Mw	1734:1735	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	9	6	theme	Da	1749:1750	arg1	weight					1726:1731	an averaged molecular weight	1704:1731	an averaged molecular weight (Mw) of 7.9×107 Da	1704:1750	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	4	7	theme	L.	692:693	arg1	1.1822					706:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	1	8	theme	extracellular	189:201	arg1	enzymes					203:209	extracellular enzymes	189:209	extracellular enzymes	189:209	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	1	8	theme	extracellular	189:201	arg1	Dextransucrases					169:183	Dextransucrases	169:183	Dextransucrases	169:183	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	6	9	theme	molecular	1170:1178	arg1	structure					1180:1188	molecular structure	1170:1188	molecular structure	1170:1188	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	4	10	theme	TMW	702:704	arg1	1.1822					706:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	11	11	theme	TMW	2106:2108	arg1	1.1822					2110:2115	L. hordei TMW 1.1822	2096:2115	L. hordei TMW 1.1822	2096:2115	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	11	12	theme	L.	2121:2122	arg1	1.1827					2136:2141	L. nagelii TMW 1.1827	2121:2141	L. nagelii TMW 1.1827	2121:2141	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	11	13	theme	L.	2096:2097	arg1	1.1822					2110:2115	L. hordei TMW 1.1822	2096:2115	L. hordei TMW 1.1822	2096:2115	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	0	14	theme	Lactobacillus	135:147	arg1	1.1827					161:166	Lactobacillus nagelii TMW 1.1827	135:166	Lactobacillus nagelii TMW 1.1827	135:166	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	3	15	theme	signal	634:639	arg1	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	0	16	theme	TMW	157:159	arg1	1.1827					161:166	Lactobacillus nagelii TMW 1.1827	135:166	Lactobacillus nagelii TMW 1.1827	135:166	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	5	17	theme	crude	940:944	arg1	enzymes					910:916	Both enzymes	905:916	Both enzymes	905:916	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	5	17	theme	crude	940:944	arg1	extracts					954:961	crude protein extracts	940:961	crude protein extracts in culture supernatants	940:985	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	11	18	theme	TMW	2132:2134	arg1	1.1827					2136:2141	L. nagelii TMW 1.1827	2121:2141	L. nagelii TMW 1.1827	2121:2141	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	8	19	theme	fine	1537:1540	arg1	structures					1542:1551	the polysaccharide fine structures	1518:1551	the polysaccharide fine structures	1518:1551	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	3	20	from	domain	605:610	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	21	theme	L.	654:655	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	0	22	dep	Lactobacillus	135:147	arg1	nagelii					149:155	nagelii	149:155	nagelii	149:155	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	0	23	theme	water	81:85	arg1	kefir					87:91	water kefir	81:91	water kefir	81:91	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	7	24	theme	transglycosylation	1227:1244	arg1	activities					1261:1270	The volumetric transglycosylation and hydrolysis activities	1212:1270	The volumetric transglycosylation and hydrolysis activities	1212:1270	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	7	24	theme	transglycosylation	1227:1244	arg1	different					1288:1296	different	1288:1296	different	1288:1296	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	11	25	theme	kefir	2084:2088	arg1	borne					2090:2094	water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827	2078:2141	borne	2090:2094	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	4	26	theme	L.	794:795	arg1	1.1827					809:814	L. nagelii TMW 1.1827	794:814	L. nagelii TMW 1.1827	794:814	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	2	27	theme	Lactobacillus	396:408	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	2	28	dep	Lactobacillus	432:444	arg1	nagelii					446:452	nagelii	446:452	nagelii	446:452	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	7	29	theme	branching	1380:1388	arg1	degree					1370:1375	a comparable degree	1357:1375	a comparable degree of branching	1357:1388	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	8	30	theme	certain	1602:1608	arg1	oligosaccharides					1610:1625	certain oligosaccharides	1602:1625	certain oligosaccharides	1602:1625	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	2	31	theme	kefir	384:388	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	5	32	theme	culture	966:972	arg1	supernatants					974:985	culture supernatants	966:985	culture supernatants	966:985	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	6	33	theme	dextrans	1072:1079	arg1	formation					1059:1067	the formation	1055:1067	the formation of dextrans	1055:1079	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	11	34	theme	kefir	2213:2217	arg1	formation					2169:2177	the complex formation	2157:2177	the complex formation of the traditional beverage water kefir	2157:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	5	35	theme	cell	1028:1031	arg1	surface					1033:1039	the cell surface	1024:1039	the cell surface	1024:1039	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	1	36	theme	linked	292:297	arg1	polymers					307:314	α-1→6 linked glucose polymers	286:314	α-1→6 linked glucose polymers from sucrose	286:327	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	10	37	theme	first	1921:1925	arg1	time					1927:1930	the first time	1917:1930	the first time for the released dextransucrase of L. nagelii TMW 1.1827	1917:1987	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	2	38	theme	TMW	417:419	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	3	39	theme	TMW	665:667	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	8	40	theme	endo-dextranase	1465:1479	arg1	digestion					1481:1489	endo-dextranase digestion	1465:1489	endo-dextranase digestion	1465:1489	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	0	41	theme	related	45:51	arg1	dextransucrases					53:67	two closely related dextransucrases	33:67	two closely related dextransucrases released by water kefir	33:91	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	6	42	theme	structural	1138:1147	arg1	analysis					1149:1156	their subsequent structural analysis	1121:1156	equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight	1084:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	10	43	theme	released	1940:1947	arg1	dextransucrase					1949:1962	the released dextransucrase	1936:1962	the released dextransucrase of L. nagelii TMW 1.1827	1936:1987	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	4	44	theme	TMW	805:807	arg1	1.1827					809:814	L. nagelii TMW 1.1827	794:814	L. nagelii TMW 1.1827	794:814	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	9	45	theme	molecular	1716:1724	arg1	Mw					1734:1735	Mw	1734:1735	Mw	1734:1735	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	9	45	theme	molecular	1716:1724	arg1	weight					1726:1731	an averaged molecular weight	1704:1731	an averaged molecular weight (Mw) of 7.9×107 Da	1704:1750	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	6	46	from	conditions	1099:1108	arg1	terms					1161:1165	terms	1161:1165	terms of molecular structure and molecular weight	1161:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	4	47	dep	1.1827	809:814	arg1	contrast					782:789	contrast	782:789	contrast	782:789	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	1	48	theme	acid	254:257	arg1	LAB					269:271	LAB	269:271	LAB	269:271	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	1	48	theme	acid	254:257	arg1	bacteria					259:266	lactic acid bacteria	247:266	lactic acid bacteria (LAB)	247:272	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	2	49	attach	derived	365:371	arg2	dextransucrases					349:363	two dextransucrases	345:363	two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827	345:463	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	2	49	attach	derived	365:371	arg1	1.1827					458:463	Lactobacillus nagelii TMW 1.1827	432:463	Lactobacillus nagelii TMW 1.1827	432:463	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	2	49	attach	derived	365:371	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	10	50	theme	nagelii	1970:1976	arg1	TMW					1978:1980	L. nagelii TMW 1.1827	1967:1987	L. nagelii TMW 1.1827	1967:1987	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	1	51	from	sucrose	321:327	arg1	polymers					307:314	α-1→6 linked glucose polymers	286:314	α-1→6 linked glucose polymers from sucrose	286:327	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	11	52	theme	beverage	2198:2205	arg1	kefir					2213:2217	the traditional beverage water kefir	2182:2217	the traditional beverage water kefir	2182:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	3	53	theme	additional	568:577	arg1	domain					605:610	an additional C-terminal glucan-binding domain	565:610	an additional C-terminal glucan-binding domain	565:610	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	53	theme	additional	568:577	arg1	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	11	54	theme	complex	2161:2167	arg1	formation					2169:2177	the complex formation	2157:2177	the complex formation of the traditional beverage water kefir	2157:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	6	55	theme	molecular	1194:1202	arg1	weight					1204:1209	molecular weight	1194:1209	molecular weight	1194:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	11	56	theme	water	2207:2211	arg1	kefir					2213:2217	the traditional beverage water kefir	2182:2217	the traditional beverage water kefir	2182:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	6	57	theme	structure	1180:1188	arg1	terms					1161:1165	terms	1161:1165	terms of molecular structure and molecular weight	1161:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	7	58	theme	hydrolysis	1250:1259	arg1	activities					1261:1270	The volumetric transglycosylation and hydrolysis activities	1212:1270	The volumetric transglycosylation and hydrolysis activities	1212:1270	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	7	58	theme	hydrolysis	1250:1259	arg1	different					1288:1296	different	1288:1296	different	1288:1296	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	11	59	theme	hordei	2099:2104	arg1	1.1822					2110:2115	L. hordei TMW 1.1822	2096:2115	L. hordei TMW 1.1822	2096:2115	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	11	60	theme	traditional	2186:2196	arg1	kefir					2213:2217	the traditional beverage water kefir	2182:2217	the traditional beverage water kefir	2182:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	4	61	theme	hordei	695:700	arg1	1.1822					706:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822	692:711	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	3	62	theme	glucan-binding	590:603	arg1	domain					605:610	an additional C-terminal glucan-binding domain	565:610	an additional C-terminal glucan-binding domain	565:610	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	62	theme	glucan-binding	590:603	arg1	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	11	63	theme	nagelii	2124:2130	arg1	1.1827					2136:2141	L. nagelii TMW 1.1827	2121:2141	L. nagelii TMW 1.1827	2121:2141	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	10	64	theme	glucansucrases	1876:1889	arg1	glycosylation					1859:1871	glycosylation	1859:1871	glycosylation of glucansucrases by LAB	1859:1896	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
31210628	8	65	theme	polysaccharide	1522:1535	arg1	structures					1542:1551	the polysaccharide fine structures	1518:1551	the polysaccharide fine structures	1518:1551	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	7	66	theme	volumetric	1216:1225	arg1	transglycosylation					1227:1244	volumetric transglycosylation	1216:1244	volumetric transglycosylation	1216:1244	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	4	67	dep	sucrose	771:777	arg1	the					741:743	the	741:743	the	741:743	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	4	67	dep	sucrose	771:777	arg1	presence					745:752	presence	745:752	presence	745:752	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	5	68	theme	protein	946:952	arg1	enzymes					910:916	Both enzymes	905:916	Both enzymes	905:916	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	5	68	theme	protein	946:952	arg1	extracts					954:961	crude protein extracts	940:961	crude protein extracts in culture supernatants	940:985	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	3	69	theme	motif	641:645	arg1	domain					605:610	an additional C-terminal glucan-binding domain	565:610	an additional C-terminal glucan-binding domain	565:610	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	69	theme	motif	641:645	arg1	presence					620:627	the presence	616:627	the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase	616:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	69	theme	motif	641:645	arg1	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	11	70	dep	borne	2090:2094	arg1	1.1822					2110:2115	L. hordei TMW 1.1822	2096:2115	L. hordei TMW 1.1822	2096:2115	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	8	71	from	differences	1503:1513	arg1	structures					1542:1551	the polysaccharide fine structures	1518:1551	the polysaccharide fine structures	1518:1551	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	8	72	theme	varying	1582:1588	arg1	portions					1590:1597	the varying portions	1578:1597	the varying portions of certain oligosaccharides	1578:1625	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	0	73	theme	Lactobacillus	99:111	arg1	1.1822					124:129	Lactobacillus hordei TMW 1.1822	99:129	Lactobacillus hordei TMW 1.1822	99:129	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	3	74	attach	presence	620:627	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	3	74	attach	presence	620:627	arg2	motif					641:645	a signal motif	632:645	a signal motif	632:645	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	9	75	theme	Da	1845:1846	arg1	Mw					1831:1832	an Mw	1828:1832	an Mw of 6.1×107 Da	1828:1846	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	0	76	theme	TMW	120:122	arg1	1.1822					124:129	Lactobacillus hordei TMW 1.1822	99:129	Lactobacillus hordei TMW 1.1822	99:129	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	0	77	dep	Lactobacillus	99:111	arg1	hordei					113:118	hordei	113:118	hordei	113:118	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	6	78	theme	reaction	1090:1097	arg1	conditions					1099:1108	equal reaction conditions	1084:1108	equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight	1084:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	8	79	theme	oligosaccharides	1610:1625	arg1	portions					1590:1597	the varying portions	1578:1597	the varying portions of certain oligosaccharides	1578:1625	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	2	80	theme	borne	390:394	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	5	81	from	extracts	954:961	arg1	supernatants					974:985	culture supernatants	966:985	culture supernatants	966:985	Both enzymes could be recovered as crude protein extracts in culture supernatants, as they are not covalently bound to the cell surface.
31210628	7	82	theme	comparable	1359:1368	arg1	degree					1370:1375	a comparable degree	1357:1375	a comparable degree of branching	1357:1388	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	2	83	theme	TMW	454:456	arg1	1.1827					458:463	Lactobacillus nagelii TMW 1.1827	432:463	Lactobacillus nagelii TMW 1.1827	432:463	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	3	84	from	presence	620:627	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	2	85	theme	water	378:382	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	1	86	theme	α-1→6	286:290	arg1	polymers					307:314	α-1→6 linked glucose polymers	286:314	α-1→6 linked glucose polymers from sucrose	286:327	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	11	87	theme	new	2018:2020	arg1	insights					2032:2039	new molecular insights	2018:2039	new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir	2018:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	2	88	theme	Lactobacillus	432:444	arg1	1.1827					458:463	Lactobacillus nagelii TMW 1.1827	432:463	Lactobacillus nagelii TMW 1.1827	432:463	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	8	89	theme	identical	1401:1409	arg1	oligosaccharides					1411:1426	identical oligosaccharides	1401:1426	identical oligosaccharides	1401:1426	Moreover, identical oligosaccharides were obtained for both dextrans upon endo-dextranase digestion, while some differences in the polysaccharide fine structures could be identified from the varying portions of certain oligosaccharides.
31210628	3	90	theme	1.1827	669:674	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	4	91	theme	substrate	761:769	arg1	sucrose					771:777	its substrate sucrose	757:777	its substrate sucrose	757:777	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	1	92	theme	glucose	299:305	arg1	polymers					307:314	α-1→6 linked glucose polymers	286:314	α-1→6 linked glucose polymers from sucrose	286:327	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	9	93	theme	L.	1683:1684	arg1	nagelii					1686:1692	L. nagelii	1683:1692	L. nagelii	1683:1692	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	7	94	theme	O3-branched	1331:1341	arg1	dextrans					1343:1350	O3-branched dextrans	1331:1350	O3-branched dextrans	1331:1350	The volumetric transglycosylation and hydrolysis activities were distinctly different for both enzymes, which produced O3-branched dextrans with a comparable degree of branching.
31210628	3	95	theme	nagelii	657:663	arg1	dextransucrase					676:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	the L. nagelii TMW 1.1827 dextransucrase	650:689	Differences between both proteins mainly arise from an additional C-terminal glucan-binding domain and the presence of a signal motif in the L. nagelii TMW 1.1827 dextransucrase.
31210628	2	96	theme	hordei	410:415	arg1	1.1822					421:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	water kefir borne Lactobacillus hordei TMW 1.1822	378:426	In this study, two dextransucrases derived from water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827 were identified and comparatively investigated.
31210628	0	97	theme	dextransucrases	53:67	arg1	comparison					19:28	comparison	19:28	comparison of two closely related dextransucrases released by water kefir	19:91	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	0	97	theme	dextransucrases	53:67	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and comparison of two closely related dextransucrases released by water kefir borne Lactobacillus hordei TMW 1.1822 and Lactobacillus nagelii TMW 1.1827.
31210628	6	98	from	analysis	1149:1156	arg1	terms					1161:1165	terms	1161:1165	terms of molecular structure and molecular weight	1161:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	9	99	theme	averaged	1707:1714	arg1	Mw					1734:1735	Mw	1734:1735	Mw	1734:1735	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	9	99	theme	averaged	1707:1714	arg1	weight					1726:1731	an averaged molecular weight	1704:1731	an averaged molecular weight (Mw) of 7.9×107 Da	1704:1750	Dextrans synthesized by the dextransucrase released by L. nagelii exhibited an averaged molecular weight (Mw) of 7.9×107 Da, while those produced by the dextransucrase released by L. hordei exhibited an Mw of 6.1×107 Da.
31210628	11	100	theme	molecular	2022:2030	arg1	insights					2032:2039	new molecular insights	2018:2039	new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir	2018:2217	Our study therefore reveals new molecular insights into how dextransucrases released by water kefir borne L. hordei TMW 1.1822 and L. nagelii TMW 1.1827 contribute to the complex formation of the traditional beverage water kefir.
31210628	6	101	theme	subsequent	1127:1136	arg1	analysis					1149:1156	their subsequent structural analysis	1121:1156	equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight	1084:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	4	102	theme	nagelii	797:803	arg1	1.1827					809:814	L. nagelii TMW 1.1827	794:814	L. nagelii TMW 1.1827	794:814	L. hordei TMW 1.1822 released the enzyme only in the presence of its substrate sucrose in contrast to L. nagelii TMW 1.1827, while both strains functionally expressed the dextransucrases independently of sucrose.
31210628	1	103	link	linked	292:297	arg1	polymers					307:314	α-1→6 linked glucose polymers	286:314	α-1→6 linked glucose polymers from sucrose	286:327	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	1	104	theme	lactic	247:252	arg1	LAB					269:271	LAB	269:271	LAB	269:271	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	1	104	theme	lactic	247:252	arg1	bacteria					259:266	lactic acid bacteria	247:266	lactic acid bacteria (LAB)	247:272	Dextransucrases are extracellular enzymes, which are exclusively expressed by lactic acid bacteria (LAB) and produce α-1→6 linked glucose polymers from sucrose.
31210628	6	105	theme	equal	1084:1088	arg1	conditions					1099:1108	equal reaction conditions	1084:1108	equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight	1084:1209	This enabled the formation of dextrans at equal reaction conditions as well as their subsequent structural analysis in terms of molecular structure and molecular weight.
31210628	10	106	theme	TMW	1978:1980	arg1	dextransucrase					1949:1962	the released dextransucrase	1936:1962	the released dextransucrase of L. nagelii TMW 1.1827	1936:1987	Moreover, glycosylation of glucansucrases by LAB was identified for the first time for the released dextransucrase of L. nagelii TMW 1.1827.
29679672	7	0	theme	reversible	1201:1210	arg1	ratio					1150:1154	The flux recovery ratio	1132:1154	The flux recovery ratio (FRR)	1132:1160	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	0	theme	reversible	1201:1210	arg1	fouling					1229:1235	reversible and irreversible fouling	1201:1235	reversible and irreversible fouling	1201:1235	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	3	1	theme	composite	511:519	arg1	membranes					524:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	7	2	theme	enhanced	1387:1394	arg1	hydrophilicity					1396:1409	enhanced hydrophilicity	1387:1409	enhanced hydrophilicity	1387:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	0	3	theme	Cellulose	0:8	arg1	membranes					28:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	4	4	theme	Filtration	676:685	arg1	characteristics					687:701	Filtration characteristics	676:701	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance	676:773	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	4	theme	Filtration	676:685	arg1	porosity					741:748	porosity	741:748	porosity	741:748	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	4	theme	Filtration	676:685	arg1	resistance					764:773	hydraulic resistance	754:773	hydraulic resistance	754:773	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	4	theme	Filtration	676:685	arg1	flux					735:738	pure water flux	724:738	pure water flux	724:738	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	3	5	theme	chemical	583:590	arg1	structure					610:618	chemical and morphological structure	583:618	chemical and morphological structure	583:618	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	1	6	theme	exfoliated	201:210	arg1	nanosheets					239:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	7	theme	disulfide	221:229	arg1	nanosheets					239:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	7	8	theme	CA	1360:1361	arg1	membrane					1363:1370	control CA membrane	1352:1370	control CA membrane	1352:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	6	9	theme	water	1075:1079	arg1	flux					1081:1084	lower water flux	1069:1084	lower water flux (92.3 ± 0.5)	1069:1097	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	6	9	theme	water	1075:1079	arg1	92.3 ± 0.5					1087:1096	92.3 ± 0.5	1087:1096	92.3 ± 0.5	1087:1096	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	5	10	theme	surface	868:874	arg1	hydrophilicity					876:889	the surface hydrophilicity	864:889	the surface hydrophilicity of composite membranes than the control CA membrane	864:941	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	4	11	theme	pure	724:727	arg1	flux					735:738	pure water flux	724:738	pure water flux	724:738	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	3	12	theme	morphological	596:608	arg1	structure					610:618	chemical and morphological structure	583:618	chemical and morphological structure	583:618	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	7	13	theme	resistance	1326:1335	arg1	result					1377:1382	a result	1375:1382	a result of enhanced hydrophilicity	1375:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	13	theme	resistance	1326:1335	arg1	potential					1337:1345	better fouling resistance potential	1311:1345	better fouling resistance potential than control CA membrane	1311:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	14	contain	possess	1303:1309	arg2	potential					1337:1345	better fouling resistance potential	1311:1345	better fouling resistance potential than control CA membrane	1311:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	14	contain	possess	1303:1309	arg1	membranes					1293:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	14	contain	possess	1303:1309	arg2	result					1377:1382	a result	1375:1382	a result of enhanced hydrophilicity	1375:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	8	15	theme	CA	1463:1464	arg1	nanosheets					1476:1485	CA and E-WS2 nanosheets	1463:1485	CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes	1463:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	7	16	theme	better	1311:1316	arg1	result					1377:1382	a result	1375:1382	a result of enhanced hydrophilicity	1375:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	16	theme	better	1311:1316	arg1	potential					1337:1345	better fouling resistance potential	1311:1345	better fouling resistance potential than control CA membrane	1311:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	0	17	theme	disulfide	72:80	arg1	nanosheets					82:91	exfoliated tungsten disulfide nanosheets	52:91	exfoliated tungsten disulfide nanosheets	52:91	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	1	18	theme	ultrafiltration	336:350	arg1	membranes					352:360	cellulose acetate (CA) ultrafiltration membranes	313:360	cellulose acetate (CA) ultrafiltration membranes	313:360	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	5	19	theme	significant	837:847	arg1	improvement					849:859	significant improvement	837:859	significant improvement	837:859	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	6	20	theme	%	1027:1027	arg1	concentration					1035:1047	1 wt% E-WS2 concentration	1023:1047	1 wt% E-WS2 concentration	1023:1047	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	8	21	theme	E-WS2	1470:1474	arg1	nanosheets					1476:1485	CA and E-WS2 nanosheets	1463:1485	CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes	1463:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	5	22	theme	CA	931:932	arg1	membrane					934:941	the control CA membrane	919:941	the control CA membrane	919:941	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	7	23	theme	serum	1170:1174	arg1	albumin					1176:1182	bovine serum albumin	1163:1182	bovine serum albumin (BSA) rejection	1163:1198	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	23	theme	serum	1170:1174	arg1	BSA					1185:1187	BSA	1185:1187	BSA	1185:1187	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	5	24	theme	nanosheets	816:825	arg1	addition					798:805	The addition	794:805	The addition of E-WS2 nanosheets	794:825	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	1	25	theme	anti-fouling	285:296	arg1	performance					298:308	anti-fouling performance	285:308	anti-fouling performance	285:308	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	7	26	theme	UF	1290:1291	arg1	membranes					1293:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	4	27	theme	hydraulic	754:762	arg1	resistance					764:773	hydraulic resistance	754:773	hydraulic resistance	754:773	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	2	28	theme	spectroscopy	440:451	arg1	terms					407:411	terms	407:411	terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD)	407:499	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	0	29	theme	composite	18:26	arg1	membranes					28:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	5	30	theme	contact	977:983	arg1	angle					985:989	the observed contact angle and porosity values	964:1009	angle	985:989	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	8	31	theme	permeation	1533:1542	arg1	characteristics					1560:1574	the permeation and antifouling characteristics	1529:1574	the permeation and antifouling characteristics of nanocomposite membranes	1529:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	3	32	theme	contact	652:658	arg1	analysis					666:673	water contact angle analysis	646:673	water contact angle analysis	646:673	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	2	33	theme	dispersive	423:432	arg1	EDXS					454:457	EDXS	454:457	EDXS	454:457	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	2	33	theme	dispersive	423:432	arg1	spectroscopy					440:451	energy dispersive X-ray spectroscopy	416:451	energy dispersive X-ray spectroscopy (EDXS)	416:458	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	7	34	theme	ratio	1150:1154	arg1	results					1250:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results	1132:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results	1132:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	1	35	theme	acetate	323:329	arg1	membranes					352:360	cellulose acetate (CA) ultrafiltration membranes	313:360	cellulose acetate (CA) ultrafiltration membranes	313:360	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	6	36	theme	CA	1050:1051	arg1	membrane					1053:1060	CA membrane	1050:1060	CA membrane	1050:1060	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	2	37	theme	diffraction	470:480	arg1	XRD					496:498	XRD	496:498	XRD	496:498	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	2	37	theme	diffraction	470:480	arg1	spectroscopy					482:493	X-ray diffraction spectroscopy	464:493	X-ray diffraction spectroscopy (XRD)	464:499	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	8	38	theme	antifouling	1548:1558	arg1	characteristics					1560:1574	the permeation and antifouling characteristics	1529:1574	the permeation and antifouling characteristics of nanocomposite membranes	1529:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	0	39	theme	exfoliated	52:61	arg1	nanosheets					82:91	exfoliated tungsten disulfide nanosheets	52:91	exfoliated tungsten disulfide nanosheets	52:91	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	1	40	theme	CA	332:333	arg1	membranes					352:360	cellulose acetate (CA) ultrafiltration membranes	313:360	cellulose acetate (CA) ultrafiltration membranes	313:360	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	6	41	theme	pore	1110:1113	arg1	effect					1124:1129	the pore plugging effect	1106:1129	the pore plugging effect	1106:1129	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	8	42	theme	membranes	1593:1601	arg1	characteristics					1560:1574	the permeation and antifouling characteristics	1529:1574	the permeation and antifouling characteristics of nanocomposite membranes	1529:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	2	43	theme	energy	416:421	arg1	EDXS					454:457	EDXS	454:457	EDXS	454:457	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	2	43	theme	energy	416:421	arg1	spectroscopy					440:451	energy dispersive X-ray spectroscopy	416:451	energy dispersive X-ray spectroscopy (EDXS)	416:458	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	3	44	theme	CA	521:522	arg1	membranes					524:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	7	45	theme	flux	1136:1139	arg1	FRR					1157:1159	FRR	1157:1159	FRR	1157:1159	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	45	theme	flux	1136:1139	arg1	ratio					1150:1154	The flux recovery ratio	1132:1154	The flux recovery ratio (FRR)	1132:1160	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	45	theme	flux	1136:1139	arg1	fouling					1229:1235	reversible and irreversible fouling	1201:1235	reversible and irreversible fouling	1201:1235	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	45	theme	flux	1136:1139	arg1	rejection					1190:1198	bovine serum albumin (BSA) rejection	1163:1198	bovine serum albumin (BSA) rejection	1163:1198	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	46	theme	hydrophilicity	1396:1409	arg1	result					1377:1382	a result	1375:1382	a result of enhanced hydrophilicity	1375:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	46	theme	hydrophilicity	1396:1409	arg1	potential					1337:1345	better fouling resistance potential	1311:1345	better fouling resistance potential than control CA membrane	1311:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	5	47	theme	composite	894:902	arg1	membranes					904:912	composite membranes	894:912	composite membranes than the control CA membrane	894:941	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	1	48	theme	tungsten	212:219	arg1	nanosheets					239:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	5	49	theme	membranes	904:912	arg1	hydrophilicity					876:889	the surface hydrophilicity	864:889	the surface hydrophilicity of composite membranes than the control CA membrane	864:941	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	8	50	theme	strong	1438:1443	arg1	interplay					1445:1453	the strong interplay	1434:1453	the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes	1434:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	7	51	theme	control	1352:1358	arg1	membrane					1363:1370	control CA membrane	1352:1370	control CA membrane	1352:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	3	52	dep	characterized	552:564	arg1	using					620:624	using	620:624	using FT-IR, XRD, SEM and water contact angle analysis	620:673	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	1	53	dep	fabrication	257:267	arg1	the					253:255	the	253:255	the	253:255	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	4	54	theme	water	729:733	arg1	flux					735:738	pure water flux	724:738	pure water flux	724:738	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	7	55	theme	experimental	1237:1248	arg1	results					1250:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results	1132:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results	1132:1256	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	6	56	theme	E-WS2	1029:1033	arg1	concentration					1035:1047	1 wt% E-WS2 concentration	1023:1047	1 wt% E-WS2 concentration	1023:1047	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	7	57	theme	fouling	1318:1324	arg1	result					1377:1382	a result	1375:1382	a result of enhanced hydrophilicity	1375:1409	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	57	theme	fouling	1318:1324	arg1	potential					1337:1345	better fouling resistance potential	1311:1345	better fouling resistance potential than control CA membrane	1311:1370	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	0	58	theme	Permeation	94:103	arg1	characteristics					105:119	Permeation characteristics	94:119	Permeation characteristics	94:119	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	1	59	theme	membranes	352:360	arg1	permeation					270:279	permeation	270:279	permeation	270:279	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	59	theme	membranes	352:360	arg1	performance					298:308	anti-fouling performance	285:308	anti-fouling performance	285:308	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	59	theme	membranes	352:360	arg1	fabrication					257:267	fabrication	257:267	fabrication	257:267	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	60	from	effects	190:196	arg1	permeation					270:279	permeation	270:279	permeation	270:279	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	60	from	effects	190:196	arg1	performance					298:308	anti-fouling performance	285:308	anti-fouling performance	285:308	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	60	from	effects	190:196	arg1	fabrication					257:267	fabrication	257:267	fabrication	257:267	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	5	61	theme	control	923:929	arg1	membrane					934:941	the control CA membrane	919:941	the control CA membrane	919:941	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	6	62	theme	1 wt	1023:1026	arg1	concentration					1035:1047	1 wt% E-WS2 concentration	1023:1047	1 wt% E-WS2 concentration	1023:1047	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	4	63	theme	membranes	706:714	arg1	characteristics					687:701	Filtration characteristics	676:701	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance	676:773	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	63	theme	membranes	706:714	arg1	porosity					741:748	porosity	741:748	porosity	741:748	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	63	theme	membranes	706:714	arg1	resistance					764:773	hydraulic resistance	754:773	hydraulic resistance	754:773	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	4	63	theme	membranes	706:714	arg1	flux					735:738	pure water flux	724:738	pure water flux	724:738	Filtration characteristics of membranes such as pure water flux, porosity and hydraulic resistance were also studied.
29679672	7	64	theme	albumin	1176:1182	arg1	ratio					1150:1154	The flux recovery ratio	1132:1154	The flux recovery ratio (FRR)	1132:1160	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	64	theme	albumin	1176:1182	arg1	rejection					1190:1198	bovine serum albumin (BSA) rejection	1163:1198	bovine serum albumin (BSA) rejection	1163:1198	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	8	65	theme	significant	1500:1510	arg1	role					1512:1515	a significant role	1498:1515	a significant role	1498:1515	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	7	66	theme	bovine	1163:1168	arg1	albumin					1176:1182	bovine serum albumin	1163:1182	bovine serum albumin (BSA) rejection	1163:1198	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	66	theme	bovine	1163:1168	arg1	BSA					1185:1187	BSA	1185:1187	BSA	1185:1187	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	5	67	theme	porosity	995:1002	arg1	values					1004:1009	the observed contact angle and porosity values	964:1009	values	1004:1009	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	0	68	theme	acetate	10:16	arg1	membranes					28:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes	0:36	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	5	69	theme	observed	968:975	arg1	angle					985:989	the observed contact angle and porosity values	964:1009	angle	985:989	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
29679672	6	70	theme	lower	1069:1073	arg1	flux					1081:1084	lower water flux	1069:1084	lower water flux (92.3 ± 0.5)	1069:1097	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	6	70	theme	lower	1069:1073	arg1	92.3 ± 0.5					1087:1096	92.3 ± 0.5	1087:1096	92.3 ± 0.5	1087:1096	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	3	71	theme	water	646:650	arg1	analysis					666:673	water contact angle analysis	646:673	water contact angle analysis	646:673	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	2	72	theme	X-ray	434:438	arg1	EDXS					454:457	EDXS	454:457	EDXS	454:457	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	2	72	theme	X-ray	434:438	arg1	spectroscopy					440:451	energy dispersive X-ray spectroscopy	416:451	energy dispersive X-ray spectroscopy (EDXS)	416:458	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	7	73	theme	recovery	1141:1148	arg1	FRR					1157:1159	FRR	1157:1159	FRR	1157:1159	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	73	theme	recovery	1141:1148	arg1	ratio					1150:1154	The flux recovery ratio	1132:1154	The flux recovery ratio (FRR)	1132:1160	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	73	theme	recovery	1141:1148	arg1	fouling					1229:1235	reversible and irreversible fouling	1201:1235	reversible and irreversible fouling	1201:1235	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	73	theme	recovery	1141:1148	arg1	rejection					1190:1198	bovine serum albumin (BSA) rejection	1163:1198	bovine serum albumin (BSA) rejection	1163:1198	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	1	74	theme	cellulose	313:321	arg1	membranes					352:360	cellulose acetate (CA) ultrafiltration membranes	313:360	cellulose acetate (CA) ultrafiltration membranes	313:360	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	7	75	theme	CA/E-WS2	1273:1280	arg1	membranes					1293:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	CA/E-WS2 (1 wt%) UF membranes	1273:1301	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	2	76	theme	spectroscopy	482:493	arg1	terms					407:411	terms	407:411	terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD)	407:499	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	0	77	theme	antifouling	125:135	arg1	ability					137:143	antifouling ability	125:143	antifouling ability	125:143	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	0	78	theme	tungsten	63:70	arg1	nanosheets					82:91	exfoliated tungsten disulfide nanosheets	52:91	exfoliated tungsten disulfide nanosheets	52:91	Cellulose acetate composite membranes tailored with exfoliated tungsten disulfide nanosheets: Permeation characteristics and antifouling ability.
29679672	2	79	theme	X-ray	464:468	arg1	XRD					496:498	XRD	496:498	XRD	496:498	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	2	79	theme	X-ray	464:468	arg1	spectroscopy					482:493	X-ray diffraction spectroscopy	464:493	X-ray diffraction spectroscopy (XRD)	464:499	The E-WS2 was prepared and characterized in terms of energy dispersive X-ray spectroscopy (EDXS) and X-ray diffraction spectroscopy (XRD).
29679672	8	80	theme	nanocomposite	1579:1591	arg1	membranes					1593:1601	nanocomposite membranes	1579:1601	nanocomposite membranes	1579:1601	This study emphasizes the strong interplay between CA and E-WS2 nanosheets which play a significant role in altering the permeation and antifouling characteristics of nanocomposite membranes.
29679672	3	81	theme	Pure	502:505	arg1	membranes					524:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes	502:532	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	6	82	theme	plugging	1115:1122	arg1	effect					1124:1129	the pore plugging effect	1106:1129	the pore plugging effect	1106:1129	However at 1 wt% E-WS2 concentration, CA membrane showed lower water flux (92.3 ± 0.5) due to the pore plugging effect.
29679672	3	83	theme	angle	660:664	arg1	analysis					666:673	water contact angle analysis	646:673	water contact angle analysis	646:673	Pure and composite CA membranes were methodically characterized for its surface, chemical and morphological structure using FT-IR, XRD, SEM and water contact angle analysis.
29679672	1	84	theme	nanosheets	239:248	arg1	effects					190:196	the effects	186:196	the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes	186:360	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	1	85	theme	E-WS2	232:236	arg1	nanosheets					239:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	exfoliated tungsten disulfide (E-WS2) nanosheets	201:248	An attempt has been made to demonstrate the effects of exfoliated tungsten disulfide (E-WS2) nanosheets on the fabrication, permeation and anti-fouling performance of cellulose acetate (CA) ultrafiltration membranes.
29679672	7	86	theme	irreversible	1216:1227	arg1	ratio					1150:1154	The flux recovery ratio	1132:1154	The flux recovery ratio (FRR)	1132:1160	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	7	86	theme	irreversible	1216:1227	arg1	fouling					1229:1235	reversible and irreversible fouling	1201:1235	reversible and irreversible fouling	1201:1235	The flux recovery ratio (FRR), bovine serum albumin (BSA) rejection, reversible and irreversible fouling experimental results suggested that CA/E-WS2 (1 wt%) UF membranes possess better fouling resistance potential than control CA membrane as a result of enhanced hydrophilicity.
29679672	5	87	theme	E-WS2	810:814	arg1	nanosheets					816:825	E-WS2 nanosheets	810:825	E-WS2 nanosheets	810:825	The addition of E-WS2 nanosheets exhibited significant improvement in the surface hydrophilicity of composite membranes than the control CA membrane and are evidenced by the observed contact angle and porosity values.
30052024	5	0	theme	human	776:780	arg1	cells					782:786	human cells	776:786	human cells	776:786	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	6	1	theme	total	942:946	arg1	prevention					948:957	the total prevention	938:957	the total prevention of biofilm formation	938:978	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	4	2	theme	deposited	712:720	arg1	bilayers					722:729	deposited bilayers	712:729	deposited bilayers	712:729	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	6	3	dep	6	1026:1026	arg1	to					1023:1024	to	1023:1024	to	1023:1024	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	1	4	theme	biopolymer-capped	187:203	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	1	4	theme	biopolymer-capped	187:203	arg1	nanoparticles					212:224	biopolymer-capped silver nanoparticles	187:224	biopolymer-capped silver nanoparticles (AgNPs)	187:232	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	5	5	theme	≥50	750:752	arg1	bilayers					754:761	≥50 bilayers	750:761	≥50 bilayers	750:761	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	5	6	theme	bilayers	754:761	arg1	bilayers					754:761	≥50 bilayers	750:761	≥50 bilayers	750:761	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	5	6	theme	bilayers	754:761	arg1	safe					768:771	safe	768:771	safe	768:771	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	5	6	theme	bilayers	754:761	arg1	composites					736:745	The composites	732:745	The composites of ≥50 bilayers	732:761	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	2	7	theme	AgNPs	285:289	arg1	dispersions					270:280	dispersions	270:280	dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC)	270:341	At first, dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound.
30052024	1	8	theme	silver	205:210	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	1	8	theme	silver	205:210	arg1	nanoparticles					212:224	biopolymer-capped silver nanoparticles	187:224	biopolymer-capped silver nanoparticles (AgNPs)	187:232	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	5	9	theme	%	808:808	arg1	activity					824:831	100% antibacterial activity	805:831	100% antibacterial activity against Staphylococcus aureus and Escherichia coli	805:882	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	3	10	theme	polycationic	405:416	arg1	nanoentities					418:429	These polycationic nanoentities	399:429	These polycationic nanoentities	399:429	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	1	11	theme	nanoparticles	212:224	arg1	deposition					173:182	the sequential deposition	158:182	the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA)	158:257	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	0	12	theme	Layer-by-Layer	10:23	arg1	Assembling					25:34	Bottom-up Layer-by-Layer Assembling	0:34	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films	0:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	7	13	theme	bacterial	1104:1112	arg1	growth					1114:1119	bacterial growth	1104:1119	bacterial growth on injured skin, burns, and chronic wounds	1104:1162	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	2	14	theme	high	372:375	arg1	ultrasound					387:396	high intensity ultrasound	372:396	high intensity ultrasound	372:396	At first, dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound.
30052024	0	15	theme	Bottom-up	0:8	arg1	Assembling					25:34	Bottom-up Layer-by-Layer Assembling	0:34	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films	0:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	1	16	theme	freestanding	105:116	arg1	films					135:139	freestanding nanobiocomposite films	105:139	freestanding nanobiocomposite films	105:139	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	4	17	theme	thickness	683:691	arg1	increase					671:678	gradual increase	663:678	gradual increase of thickness	663:691	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	6	18	theme	surface	999:1005	arg1	adherence					1007:1015	the cells surface adherence	989:1015	the cells surface adherence	989:1015	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	1	19	theme	nanobiocomposite	118:133	arg1	films					135:139	freestanding nanobiocomposite films	105:139	freestanding nanobiocomposite films	105:139	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	4	20	theme	assemblies	643:652	arg1	images					629:634	SEM images	625:634	SEM images of the assemblies	625:652	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	3	21	theme	active	588:593	arg1	agent					595:599	active agent	588:599	active agent	588:599	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	6	22	theme	cells	993:997	arg1	adherence					1007:1015	the cells surface adherence	989:1015	the cells surface adherence	989:1015	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	3	23	theme	biopolymer-capped	542:558	arg1	AgNPs					560:564	the biopolymer-capped AgNPs	538:564	the biopolymer-capped AgNPs	538:564	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	6	24	contain	containing	905:914	arg1	films					899:903	the films	895:903	the films containing CSAgNPs	895:922	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	6	24	contain	containing	905:914	arg2	CSAgNPs					916:922	CSAgNPs	916:922	CSAgNPs	916:922	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	0	25	theme	Freestanding	53:64	arg1	Films					83:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	4	26	theme	bilayers	722:729	arg1	number					702:707	the number	698:707	the number of deposited bilayers	698:729	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	7	27	theme	effective	1075:1083	arg1	barrier					1085:1091	an effective barrier	1072:1091	an effective barrier	1072:1091	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	7	27	theme	effective	1075:1083	arg1	nanobiocomposites					1039:1055	Such nanobiocomposites	1034:1055	Such nanobiocomposites	1034:1055	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	4	28	theme	gradual	663:669	arg1	increase					671:678	gradual increase	663:678	gradual increase of thickness	663:691	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	3	29	theme	structural	605:614	arg1	element					616:622	structural element	605:622	structural element	605:622	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	1	30	theme	hyaluronic	238:247	arg1	HA					255:256	HA	255:256	HA	255:256	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	1	30	theme	hyaluronic	238:247	arg1	acid					249:252	hyaluronic acid	238:252	hyaluronic acid (HA)	238:257	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	0	31	theme	Antibacterial	39:51	arg1	Films					83:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	7	32	theme	Such	1034:1037	arg1	barrier					1085:1091	an effective barrier	1072:1091	an effective barrier	1072:1091	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	7	32	theme	Such	1034:1037	arg1	nanobiocomposites					1039:1055	Such nanobiocomposites	1034:1055	Such nanobiocomposites	1034:1055	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	3	33	theme	HA	470:471	arg1	polyanion					473:481	the HA polyanion	466:481	the HA polyanion	466:481	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	0	34	theme	Films	83:87	arg1	Assembling					25:34	Bottom-up Layer-by-Layer Assembling	0:34	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films	0:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	6	35	theme	formation	970:978	arg1	prevention					948:957	the total prevention	938:957	the total prevention of biofilm formation	938:978	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	7	36	from	growth	1114:1119	arg1	wounds					1157:1162	chronic wounds	1149:1162	chronic wounds	1149:1162	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	7	36	from	growth	1114:1119	arg1	burns					1138:1142	burns	1138:1142	burns	1138:1142	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	7	36	from	growth	1114:1119	arg1	skin					1132:1135	injured skin	1124:1135	injured skin	1124:1135	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	3	37	theme	agent	595:599	arg1	role					580:583	the dual role	571:583	the dual role of active agent	571:599	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	3	37	theme	agent	595:599	arg1	element					616:622	structural element	605:622	structural element	605:622	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	3	38	theme	3D	502:503	arg1	constructs					520:529	stable 3D supramolecular constructs	495:529	stable 3D supramolecular constructs	495:529	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	0	39	theme	Nanobiocomposite	66:81	arg1	Films					83:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Antibacterial Freestanding Nanobiocomposite Films	39:87	Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films.
30052024	6	40	theme	biofilm	962:968	arg1	formation					970:978	biofilm formation	962:978	biofilm formation	962:978	Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs.
30052024	7	41	theme	injured	1124:1130	arg1	skin					1132:1135	injured skin	1124:1135	injured skin	1124:1135	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	3	42	theme	supramolecular	505:518	arg1	constructs					520:529	stable 3D supramolecular constructs	495:529	stable 3D supramolecular constructs	495:529	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	7	43	theme	chronic	1149:1155	arg1	wounds					1157:1162	chronic wounds	1149:1162	chronic wounds	1149:1162	Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.
30052024	3	44	theme	dual	575:578	arg1	role					580:583	the dual role	571:583	the dual role of active agent	571:599	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	4	45	theme	SEM	625:627	arg1	images					629:634	SEM images	625:634	SEM images of the assemblies	625:652	SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers.
30052024	3	46	theme	stable	495:500	arg1	constructs					520:529	stable 3D supramolecular constructs	495:529	stable 3D supramolecular constructs	495:529	These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element.
30052024	1	47	theme	acid	249:252	arg1	deposition					173:182	the sequential deposition	158:182	the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA)	158:257	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	1	48	theme	sequential	162:171	arg1	deposition					173:182	the sequential deposition	158:182	the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA)	158:257	In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA).
30052024	2	49	theme	intensity	377:385	arg1	ultrasound					387:396	high intensity ultrasound	372:396	high intensity ultrasound	372:396	At first, dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound.
30052024	5	50	theme	100	805:807	arg1	%					808:808	%	808:808	%	808:808	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30052024	5	51	theme	antibacterial	810:822	arg1	activity					824:831	100% antibacterial activity	805:831	100% antibacterial activity against Staphylococcus aureus and Escherichia coli	805:882	The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli.
30933903	5	0	theme	host-guest	731:740	arg1	interaction					752:762	The host-guest inclusion interaction	727:762	The host-guest inclusion interaction	727:762	The host-guest inclusion interaction was detected by ultraviolet spectroscopy and DPV.
30933903	8	1	theme	electrochemical	1129:1143	arg1	sensor					1145:1150	this electrochemical sensor	1124:1150	this electrochemical sensor	1124:1150	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine were obtained for this electrochemical sensor.
30933903	9	2	theme	as-synthesized	1157:1170	arg1	material					1172:1179	The as-synthesized material	1153:1179	The as-synthesized material	1153:1179	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	0	3	theme	phenylalanine	110:122	arg1	enantiomers					124:134	phenylalanine enantiomers	110:134	phenylalanine enantiomers	110:134	Graphene-ferrocene functionalized cyclodextrin composite with high electrochemical recognition capability for phenylalanine enantiomers.
30933903	9	4	theme	application	1294:1304	arg1	prospects					1306:1314	wide application prospects	1289:1314	wide application prospects	1289:1314	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	6	5	theme	recognition	897:907	arg1	capability					909:918	a higher chiral recognition capability	881:918	a higher chiral recognition capability for L-Phe	881:928	The recognition results demonstrated that the rGO-Fc-CD/GCE showed a higher chiral recognition capability for L-Phe than for D-Phe.
30933903	6	6	theme	chiral	890:895	arg1	capability					909:918	a higher chiral recognition capability	881:918	a higher chiral recognition capability for L-Phe	881:928	The recognition results demonstrated that the rGO-Fc-CD/GCE showed a higher chiral recognition capability for L-Phe than for D-Phe.
30933903	8	7	theme	LOD	1026:1028	arg1	values					1030:1035	The LOD values	1022:1035	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine	1022:1104	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine were obtained for this electrochemical sensor.
30933903	1	8	theme	large	269:273	arg1	area					283:286	large surface area	269:286	large surface area	269:286	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	6	9	theme	higher	883:888	arg1	capability					909:918	a higher chiral recognition capability	881:918	a higher chiral recognition capability for L-Phe	881:928	The recognition results demonstrated that the rGO-Fc-CD/GCE showed a higher chiral recognition capability for L-Phe than for D-Phe.
30933903	8	10	theme	27 nM	1040:1044	arg1	values					1030:1035	The LOD values	1022:1035	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine	1022:1104	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine were obtained for this electrochemical sensor.
30933903	3	11	theme	chiral	453:458	arg1	interface					484:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	3	12	theme	rGO-Fc-CD	523:531	arg1	modification					560:571	the electrode modification	546:571	the electrode modification for the recognition of phenylalanine (Phe) enantiomers	546:626	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	3	12	theme	rGO-Fc-CD	523:531	arg1	composite					533:541	the rGO-Fc-CD composite	519:541	the rGO-Fc-CD composite	519:541	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	4	13	theme	composites	661:670	arg1	synthesis					644:652	The successful synthesis	629:652	The successful synthesis of the composites	629:670	The successful synthesis of the composites was confirmed by FTIR, XRD, TGA, SEM, and XPS results.
30933903	2	14	theme	functionalized	372:385	arg1	GO-Fc					387:391	β-cyclodextrin (β-CD) functionalized GO-Fc	350:391	β-cyclodextrin (β-CD) functionalized GO-Fc	350:391	Then, a novel composite was synthesized via β-cyclodextrin (β-CD) functionalized GO-Fc by combining the advantages of GO-Fc and β-CD.
30933903	2	15	theme	β-CD	434:437	arg1	advantages					410:419	the advantages	406:419	the advantages of GO-Fc and β-CD	406:437	Then, a novel composite was synthesized via β-cyclodextrin (β-CD) functionalized GO-Fc by combining the advantages of GO-Fc and β-CD.
30933903	5	16	theme	inclusion	742:750	arg1	interaction					752:762	The host-guest inclusion interaction	727:762	The host-guest inclusion interaction	727:762	The host-guest inclusion interaction was detected by ultraviolet spectroscopy and DPV.
30933903	8	17	theme	52 nM	1050:1054	arg1	values					1030:1035	The LOD values	1022:1035	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine	1022:1104	The LOD values of 27 nM and 52 nM (S/N = 3) for L-Phenylalanine and D-Phenylalanine were obtained for this electrochemical sensor.
30933903	9	18	contain	has	1285:1287	arg2	prospects					1306:1314	wide application prospects	1289:1314	wide application prospects	1289:1314	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	9	18	contain	has	1285:1287	arg1	sensor					1278:1283	the developed sensor	1264:1283	the developed sensor	1264:1283	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	1	19	theme	surface	275:281	arg1	area					283:286	large surface area	269:286	large surface area	269:286	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	1	20	theme	Graphene	137:144	arg1	oxide					146:150	Graphene oxide	137:150	Graphene oxide (GO) modified with ferrocene (Fc)	137:184	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	2	21	theme	GO-Fc	424:428	arg1	advantages					410:419	the advantages	406:419	the advantages of GO-Fc and β-CD	406:437	Then, a novel composite was synthesized via β-cyclodextrin (β-CD) functionalized GO-Fc by combining the advantages of GO-Fc and β-CD.
30933903	1	22	theme	area	283:286	arg1	features					257:264	the features	253:264	the features of large surface area and high loading	253:303	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	7	23	theme	enantioselectivity	950:967	arg1	ID/IL					982:986	ID/IL	982:986	ID/IL	982:986	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	7	23	theme	enantioselectivity	950:967	arg1	coefficient					969:979	The enantioselectivity coefficient	946:979	The enantioselectivity coefficient (ID/IL) of the proposed sensor	946:1010	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	7	23	theme	enantioselectivity	950:967	arg1	2.47					1016:1019	2.47	1016:1019	2.47	1016:1019	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	3	24	theme	electrode	550:558	arg1	modification					560:571	the electrode modification	546:571	the electrode modification for the recognition of phenylalanine (Phe) enantiomers	546:626	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	3	24	theme	electrode	550:558	arg1	composite					533:541	the rGO-Fc-CD composite	519:541	the rGO-Fc-CD composite	519:541	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	3	25	theme	efficient	443:451	arg1	interface					484:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	0	26	theme	cyclodextrin	34:45	arg1	composite					47:55	cyclodextrin composite	34:55	cyclodextrin composite	34:55	Graphene-ferrocene functionalized cyclodextrin composite with high electrochemical recognition capability for phenylalanine enantiomers.
30933903	9	27	theme	Phe	1231:1233	arg1	enantiomers					1235:1245	Phe enantiomers	1231:1245	Phe enantiomers	1231:1245	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	4	28	theme	XPS	714:716	arg1	results					718:724	XPS results	714:724	XPS results	714:724	The successful synthesis of the composites was confirmed by FTIR, XRD, TGA, SEM, and XPS results.
30933903	1	29	theme	π-π	221:223	arg1	GO-Fc					238:242	GO-Fc	238:242	GO-Fc	238:242	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	1	29	theme	π-π	221:223	arg1	interaction					225:235	the π-π interaction	217:235	the π-π interaction (GO-Fc)	217:243	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	0	30	theme	high	62:65	arg1	capability					95:104	high electrochemical recognition capability	62:104	high electrochemical recognition capability for phenylalanine enantiomers	62:134	Graphene-ferrocene functionalized cyclodextrin composite with high electrochemical recognition capability for phenylalanine enantiomers.
30933903	1	31	theme	high	292:295	arg1	loading					297:303	high loading	292:303	high loading	292:303	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	9	32	theme	enantiomers	1235:1245	arg1	recognition					1216:1226	the recognition	1212:1226	the recognition of Phe enantiomers	1212:1245	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	3	33	theme	phenylalanine	596:608	arg1	enantiomers					616:626	phenylalanine (Phe) enantiomers	596:626	phenylalanine (Phe) enantiomers	596:626	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	1	34	theme	loading	297:303	arg1	features					257:264	the features	253:264	the features of large surface area and high loading	253:303	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	0	35	theme	recognition	83:93	arg1	capability					95:104	high electrochemical recognition capability	62:104	high electrochemical recognition capability for phenylalanine enantiomers	62:134	Graphene-ferrocene functionalized cyclodextrin composite with high electrochemical recognition capability for phenylalanine enantiomers.
30933903	9	36	theme	wide	1289:1292	arg1	prospects					1306:1314	wide application prospects	1289:1314	wide application prospects	1289:1314	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	0	37	theme	electrochemical	67:81	arg1	capability					95:104	high electrochemical recognition capability	62:104	high electrochemical recognition capability for phenylalanine enantiomers	62:134	Graphene-ferrocene functionalized cyclodextrin composite with high electrochemical recognition capability for phenylalanine enantiomers.
30933903	7	38	theme	proposed	996:1003	arg1	sensor					1005:1010	the proposed sensor	992:1010	the proposed sensor	992:1010	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	6	39	theme	recognition	818:828	arg1	results					830:836	The recognition results	814:836	The recognition results	814:836	The recognition results demonstrated that the rGO-Fc-CD/GCE showed a higher chiral recognition capability for L-Phe than for D-Phe.
30933903	3	40	theme	enantiomers	616:626	arg1	recognition					581:591	the recognition	577:591	the recognition of phenylalanine (Phe) enantiomers	577:626	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	7	41	theme	sensor	1005:1010	arg1	ID/IL					982:986	ID/IL	982:986	ID/IL	982:986	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	7	41	theme	sensor	1005:1010	arg1	coefficient					969:979	The enantioselectivity coefficient	946:979	The enantioselectivity coefficient (ID/IL) of the proposed sensor	946:1010	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	7	41	theme	sensor	1005:1010	arg1	2.47					1016:1019	2.47	1016:1019	2.47	1016:1019	The enantioselectivity coefficient (ID/IL) of the proposed sensor was 2.47.
30933903	2	42	theme	novel	314:318	arg1	composite					320:328	a novel composite	312:328	a novel composite	312:328	Then, a novel composite was synthesized via β-cyclodextrin (β-CD) functionalized GO-Fc by combining the advantages of GO-Fc and β-CD.
30933903	9	43	theme	developed	1268:1276	arg1	sensor					1278:1283	the developed sensor	1264:1283	the developed sensor	1264:1283	The as-synthesized material was successfully exploited for the recognition of Phe enantiomers, indicating that the developed sensor has wide application prospects.
30933903	3	44	theme	sensing	476:482	arg1	interface					484:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	3	45	theme	electrochemical	460:474	arg1	interface					484:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface	440:492	An efficient chiral electrochemical sensing interface was constructed by using the rGO-Fc-CD composite as the electrode modification for the recognition of phenylalanine (Phe) enantiomers.
30933903	4	46	theme	successful	633:642	arg1	synthesis					644:652	The successful synthesis	629:652	The successful synthesis of the composites	629:670	The successful synthesis of the composites was confirmed by FTIR, XRD, TGA, SEM, and XPS results.
30933903	1	47	dep	oxide	146:150	arg1	GO					153:154	GO	153:154	GO	153:154	Graphene oxide (GO) modified with ferrocene (Fc) was successfully assembled via the π-π interaction (GO-Fc) and had the features of large surface area and high loading.
30933903	5	48	theme	ultraviolet	780:790	arg1	spectroscopy					792:803	ultraviolet spectroscopy	780:803	ultraviolet spectroscopy	780:803	The host-guest inclusion interaction was detected by ultraviolet spectroscopy and DPV.
31843616	0	0	theme	carboxymethyl	71:83	arg1	synthesis					95:103	carboxymethyl cellulose synthesis	71:103	carboxymethyl cellulose synthesis	71:103	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	0	theme	carboxymethyl	71:83	arg1	hulls					8:12	Soybean hulls	0:12	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.	0:104	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	4	1	theme	pulp	552:555	arg1	process					567:573	the pulp bleaching process	548:573	the pulp bleaching process	548:573	Volumes of bleaching solution (VS) of between 55 and 65 mL/g at temperatures between 85 and 95 °C and VS of 70 and 75 mL/g at 95 °C were applied in the pulp bleaching process.
31843616	5	2	theme	chloroacetic	634:645	arg1	factors					580:586	The factors	576:586	The factors considered in the carboxymethylation	576:623	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	2	theme	chloroacetic	634:645	arg1	1.2-2.1 g/g					658:668	1.2-2.1 g/g	658:668	1.2-2.1 g/g	658:668	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	2	theme	chloroacetic	634:645	arg1	mass					652:655	the chloroacetic acid mass	630:655	the chloroacetic acid mass (1.2-2.1 g/g)	630:669	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	9	3	theme	TGA/DTG	1047:1053	arg1	analysis					1055:1062	TGA/DTG analysis	1047:1062	TGA/DTG analysis	1047:1062	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	8	4	theme	crystallinity	1001:1013	arg1	degree					991:996	a high degree	984:996	a high degree of crystallinity	984:1013	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	9	5	theme	1H	1025:1026	arg1	NMR					1028:1030	1H NMR	1025:1030	1H NMR	1025:1030	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	6	6	contain	have	768:771	arg1	hulls					730:734	The soybean hulls	718:734	The soybean hulls	718:734	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	6	6	contain	have	768:771	arg2	content					786:792	a low lignin content	773:792	a low lignin content	773:792	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	2	7	dep	%	246:246	arg1	solutions					267:275	NaOH solutions	262:275	NaOH solutions	262:275	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	9	8	theme	analysis	1055:1062	arg1	results					1064:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	0	9	theme	cellulose	85:93	arg1	synthesis					95:103	carboxymethyl cellulose synthesis	71:103	carboxymethyl cellulose synthesis	71:103	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	9	theme	cellulose	85:93	arg1	hulls					8:12	Soybean hulls	0:12	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.	0:104	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	3	10	used	used	329:332	arg2	design					318:323	A 22 central composite design	295:323	A 22 central composite design	295:323	A 22 central composite design was used in order to optimize the bleaching process and the CMC synthesis.
31843616	1	11	theme	high	147:150	arg1	volumes					152:158	high volumes	147:158	high volumes	147:158	Soybean hulls, a co-product generated in high volumes, were used to obtain pulp and CMC.
31843616	3	12	theme	CMC	385:387	arg1	synthesis					389:397	the CMC synthesis	381:397	the CMC synthesis	381:397	A 22 central composite design was used in order to optimize the bleaching process and the CMC synthesis.
31843616	9	13	theme	chloroacetic	1182:1193	arg1	acid					1195:1198	chloroacetic acid	1182:1198	chloroacetic acid	1182:1198	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	0	14	theme	pulping	35:41	arg1	processes					57:65	the pulping and bleaching processes	31:65	the pulping and bleaching processes	31:65	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	6	15	theme	soybean	722:728	arg1	hulls					730:734	The soybean hulls	718:734	The soybean hulls	718:734	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	7	16	used	used	846:849	arg2	NaOH					837:840	1% NaOH	834:840	1% NaOH	834:840	The pulping process was optimized when 1% NaOH was used at 90 °C/2 h and bleaching process applying VS = 75 mL at 95 °C/4 h.
31843616	9	17	theme	acid	1195:1198	arg1	2.1 g					1173:1177	2.1 g	1173:1177	2.1 g of chloroacetic acid	1173:1198	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	2	18	dep	the	234:236	arg1	aid					238:240	aid	238:240	aid	238:240	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	0	19	theme	Soybean	0:6	arg1	synthesis					95:103	carboxymethyl cellulose synthesis	71:103	carboxymethyl cellulose synthesis	71:103	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	19	theme	Soybean	0:6	arg1	Optimization					15:26	Optimization	15:26	Optimization of the pulping and bleaching processes	15:65	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	19	theme	Soybean	0:6	arg1	hulls					8:12	Soybean hulls	0:12	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.	0:104	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	9	20	theme	bleached	1154:1161	arg1	pulp					1163:1166	the bleached pulp	1150:1166	the bleached pulp with 2.1 g of chloroacetic acid for 192 min	1150:1210	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	8	21	theme	high	986:989	arg1	degree					991:996	a high degree	984:996	a high degree of crystallinity	984:1013	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	2	22	from	90 °C	280:284	arg1	%					260:260	2.5%	257:260	2.5%	257:260	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	2	22	from	90 °C	280:284	arg1	%					246:246	1%	245:246	1%	245:246	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	2	22	from	90 °C	280:284	arg1	%					250:250	2%	249:250	2%	249:250	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	3	23	theme	bleaching	359:367	arg1	process					369:375	the bleaching process	355:375	the bleaching process	355:375	A 22 central composite design was used in order to optimize the bleaching process and the CMC synthesis.
31843616	5	24	theme	reaction	679:686	arg1	192-228 min					694:704	192-228 min	694:704	192-228 min	694:704	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	24	theme	reaction	679:686	arg1	time					688:691	the reaction time	675:691	the reaction time (192-228 min)	675:705	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	1	25	theme	Soybean	106:112	arg1	hulls					114:118	Soybean hulls	106:118	Soybean hulls	106:118	Soybean hulls, a co-product generated in high volumes, were used to obtain pulp and CMC.
31843616	1	25	theme	Soybean	106:112	arg1	co-product					123:132	a co-product	121:132	a co-product generated in high volumes	121:158	Soybean hulls, a co-product generated in high volumes, were used to obtain pulp and CMC.
31843616	5	26	theme	acid	647:650	arg1	factors					580:586	The factors	576:586	The factors considered in the carboxymethylation	576:623	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	26	theme	acid	647:650	arg1	1.2-2.1 g/g					658:668	1.2-2.1 g/g	658:668	1.2-2.1 g/g	658:668	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	26	theme	acid	647:650	arg1	mass					652:655	the chloroacetic acid mass	630:655	the chloroacetic acid mass (1.2-2.1 g/g)	630:669	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	6	27	theme	lignin	779:784	arg1	content					786:792	a low lignin content	773:792	a low lignin content	773:792	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	9	28	theme	SEM	1020:1022	arg1	results					1064:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	6	29	theme	low	775:777	arg1	content					786:792	a low lignin content	773:792	a low lignin content	773:792	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	7	30	theme	bleaching	868:876	arg1	process					878:884	bleaching process	868:884	bleaching process	868:884	The pulping process was optimized when 1% NaOH was used at 90 °C/2 h and bleaching process applying VS = 75 mL at 95 °C/4 h.
31843616	2	31	theme	pulping	199:205	arg1	process					207:213	The pulping process	195:213	The pulping process	195:213	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	7	32	theme	%	835:835	arg1	NaOH					837:840	1% NaOH	834:840	1% NaOH	834:840	The pulping process was optimized when 1% NaOH was used at 90 °C/2 h and bleaching process applying VS = 75 mL at 95 °C/4 h.
31843616	4	33	theme	70	508:509	arg1	solution					421:428	solution	421:428	solution	421:428	Volumes of bleaching solution (VS) of between 55 and 65 mL/g at temperatures between 85 and 95 °C and VS of 70 and 75 mL/g at 95 °C were applied in the pulp bleaching process.
31843616	4	33	theme	70	508:509	arg1	75 mL/g					515:521	75 mL/g	515:521	75 mL/g	515:521	Volumes of bleaching solution (VS) of between 55 and 65 mL/g at temperatures between 85 and 95 °C and VS of 70 and 75 mL/g at 95 °C were applied in the pulp bleaching process.
31843616	3	34	theme	central	300:306	arg1	design					318:323	A 22 central composite design	295:323	A 22 central composite design	295:323	A 22 central composite design was used in order to optimize the bleaching process and the CMC synthesis.
31843616	8	35	theme	lignin	941:946	arg1	content					948:954	low lignin content	937:954	low lignin content (<6%)	937:960	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	8	35	theme	lignin	941:946	arg1	%					959:959	<6%	957:959	<6%	957:959	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	2	36	theme	NaOH	262:265	arg1	solutions					267:275	NaOH solutions	262:275	NaOH solutions	262:275	The pulping process was optimized with the aid of 1%, 2%, and 2.5% NaOH solutions at 90 °C for 2 h.
31843616	3	37	theme	composite	308:316	arg1	design					318:323	A 22 central composite design	295:323	A 22 central composite design	295:323	A 22 central composite design was used in order to optimize the bleaching process and the CMC synthesis.
31843616	9	38	theme	FTIR	1038:1041	arg1	results					1064:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	9	39	theme	NMR	1028:1030	arg1	results					1064:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	9	40	with	pulp	1163:1166	arg1	2.1 g					1173:1177	2.1 g	1173:1177	2.1 g of chloroacetic acid	1173:1198	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	4	41	theme	bleaching	557:565	arg1	process					567:573	the pulp bleaching process	548:573	the pulp bleaching process	548:573	Volumes of bleaching solution (VS) of between 55 and 65 mL/g at temperatures between 85 and 95 °C and VS of 70 and 75 mL/g at 95 °C were applied in the pulp bleaching process.
31843616	8	42	theme	low	937:939	arg1	content					948:954	low lignin content	937:954	low lignin content (<6%)	937:960	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	8	42	theme	low	937:939	arg1	%					959:959	<6%	957:959	<6%	957:959	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	7	43	theme	1	834:834	arg1	%					835:835	%	835:835	%	835:835	The pulping process was optimized when 1% NaOH was used at 90 °C/2 h and bleaching process applying VS = 75 mL at 95 °C/4 h.
31843616	9	44	theme	XRD	1033:1035	arg1	results					1064:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results	1016:1070	The SEM, 1H NMR, XRD, FTIR and TGA/DTG analysis results demonstrated that it is possible to synthesize CMC (DS = 1.45) by acetylating the bleached pulp with 2.1 g of chloroacetic acid for 192 min, at 63 °C.
31843616	1	45	used	used	166:169	arg2	hulls					114:118	Soybean hulls	106:118	Soybean hulls	106:118	Soybean hulls, a co-product generated in high volumes, were used to obtain pulp and CMC.
31843616	1	45	used	used	166:169	arg2	co-product					123:132	a co-product	121:132	a co-product generated in high volumes	121:158	Soybean hulls, a co-product generated in high volumes, were used to obtain pulp and CMC.
31843616	5	46	from	63 °C	711:715	arg1	192-228 min					694:704	192-228 min	694:704	192-228 min	694:704	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	46	from	63 °C	711:715	arg1	time					688:691	the reaction time	675:691	the reaction time (192-228 min)	675:705	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	46	from	63 °C	711:715	arg1	1.2-2.1 g/g					658:668	1.2-2.1 g/g	658:668	1.2-2.1 g/g	658:668	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	46	from	63 °C	711:715	arg1	mass					652:655	the chloroacetic acid mass	630:655	the chloroacetic acid mass (1.2-2.1 g/g)	630:669	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	5	46	from	63 °C	711:715	arg1	factors					580:586	The factors	576:586	The factors considered in the carboxymethylation	576:623	The factors considered in the carboxymethylation were the chloroacetic acid mass (1.2-2.1 g/g) and the reaction time (192-228 min), at 63 °C.
31843616	0	47	theme	processes	57:65	arg1	synthesis					95:103	carboxymethyl cellulose synthesis	71:103	carboxymethyl cellulose synthesis	71:103	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	47	theme	processes	57:65	arg1	Optimization					15:26	Optimization	15:26	Optimization of the pulping and bleaching processes	15:65	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	47	theme	processes	57:65	arg1	hulls					8:12	Soybean hulls	0:12	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.	0:104	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	6	48	contain	contain	736:742	arg1	hulls					730:734	The soybean hulls	718:734	The soybean hulls	718:734	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	6	48	contain	contain	736:742	arg2	cellulose					754:762	cellulose	754:762	cellulose	754:762	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	6	48	contain	contain	736:742	arg2	%					749:749	40.62%	744:749	40.62% of cellulose	744:762	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	0	49	dep	hulls	8:12	arg1	synthesis					95:103	carboxymethyl cellulose synthesis	71:103	carboxymethyl cellulose synthesis	71:103	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	49	dep	hulls	8:12	arg1	Optimization					15:26	Optimization	15:26	Optimization of the pulping and bleaching processes	15:65	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	49	dep	hulls	8:12	arg1	hulls					8:12	Soybean hulls	0:12	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.	0:104	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	0	50	theme	bleaching	47:55	arg1	processes					57:65	the pulping and bleaching processes	31:65	the pulping and bleaching processes	31:65	Soybean hulls: Optimization of the pulping and bleaching processes and carboxymethyl cellulose synthesis.
31843616	6	51	theme	cellulose	754:762	arg1	%					749:749	40.62%	744:749	40.62% of cellulose	744:762	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	6	51	theme	cellulose	754:762	arg1	cellulose					754:762	cellulose	754:762	cellulose	754:762	The soybean hulls contain 40.62% of cellulose and have a low lignin content.
31843616	8	52	contain	had	980:982	arg1	cellulose					970:978	the cellulose	966:978	the cellulose	966:978	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	8	52	contain	had	980:982	arg2	degree					991:996	a high degree	984:996	a high degree of crystallinity	984:1013	The pulps showed low lignin content (<6%) and the cellulose had a high degree of crystallinity.
31843616	7	53	theme	pulping	799:805	arg1	process					807:813	The pulping process	795:813	The pulping process	795:813	The pulping process was optimized when 1% NaOH was used at 90 °C/2 h and bleaching process applying VS = 75 mL at 95 °C/4 h.
31843616	4	54	from	temperatures	464:475	arg1	55					446:447	55	446:447	55	446:447	Volumes of bleaching solution (VS) of between 55 and 65 mL/g at temperatures between 85 and 95 °C and VS of 70 and 75 mL/g at 95 °C were applied in the pulp bleaching process.
29701959	2	0	theme	synergetic	513:522	arg1	effects					524:530	some intestinal synergetic effects	497:530	some intestinal synergetic effects	497:530	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	2	1	theme	intestinal	502:511	arg1	effects					524:530	some intestinal synergetic effects	497:530	some intestinal synergetic effects	497:530	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	4	2	theme	relative	829:836	arg1	abundances					838:847	the relative abundances	825:847	the relative abundances of family Rikenellaceae and order Clostridiales	825:895	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	5	3	theme	MOS	1052:1054	arg1	usage					1043:1047	usage	1043:1047	usage of MOS	1043:1054	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	3	4	theme	glucose	673:679	arg1	tolerance					681:689	glucose tolerance	673:689	glucose tolerance	673:689	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	4	5	theme	S24-7	944:948	arg1	OTU05945					925:932	an unnamed OTU05945	914:932	an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum	914:1007	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	4	6	theme	Clostridiales	883:895	arg1	abundances					838:847	the relative abundances	825:847	the relative abundances of family Rikenellaceae and order Clostridiales	825:895	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	1	7	theme	Type	127:130	arg1	T2D					144:146	T2D	144:146	T2D	144:146	Type 2 diabetes (T2D) induced by obesity and high-fat diet is significantly associated with gut microbiota dysbacteriosis.
29701959	1	7	theme	Type	127:130	arg1	diabetes					134:141	Type 2 diabetes	127:141	Type 2 diabetes (T2D) induced by obesity and high-fat diet	127:184	Type 2 diabetes (T2D) induced by obesity and high-fat diet is significantly associated with gut microbiota dysbacteriosis.
29701959	0	8	theme	Gut	111:113	arg1	Microbiota					115:124	Gut Microbiota	111:124	Gut Microbiota	111:124	Prebiotic Mannan-Oligosaccharides Augment the Hypoglycemic Effects of Metformin in Correlation with Modulating Gut Microbiota.
29701959	4	9	theme	family	937:942	arg1	S24-7					944:948	family S24-7	937:948	family S24-7	937:948	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	2	10	theme	metformin	294:302	arg1	medicine					282:289	the first line clinical medicine	258:289	the first line clinical medicine of metformin	258:302	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	4	11	theme	community	775:783	arg1	composition					785:795	the gut community composition	767:795	the gut community composition	767:795	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	0	12	from	Effects	59:65	arg1	Correlation					83:93	Correlation	83:93	Correlation	83:93	Prebiotic Mannan-Oligosaccharides Augment the Hypoglycemic Effects of Metformin in Correlation with Modulating Gut Microbiota.
29701959	4	13	theme	family	852:857	arg1	Rikenellaceae					859:871	family Rikenellaceae	852:871	family Rikenellaceae	852:871	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	3	14	theme	Composite	560:568	arg1	treatment					570:578	Composite treatment	560:578	Composite treatment of metformin and MOS	560:599	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	5	15	with	association	1111:1121	arg1	modulation					1143:1152	gut microbiota modulation	1128:1152	gut microbiota modulation	1128:1152	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	1	16	theme	high-fat	172:179	arg1	diet					181:184	high-fat diet	172:184	high-fat diet	172:184	Type 2 diabetes (T2D) induced by obesity and high-fat diet is significantly associated with gut microbiota dysbacteriosis.
29701959	5	17	theme	further	1190:1196	arg1	medication					1198:1207	further medication	1190:1207	further medication	1190:1207	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	4	18	theme	unnamed	917:923	arg1	OTU05945					925:932	an unnamed OTU05945	914:932	an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum	914:1007	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	3	19	theme	MOS	597:599	arg1	treatment					570:578	Composite treatment	560:578	Composite treatment of metformin and MOS	560:599	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	2	20	theme	mannan-oligosaccharides	396:418	arg1	prebiotic					376:384	a prebiotic	374:384	a prebiotic of konjac mannan-oligosaccharides (MOS)	374:424	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	0	21	theme	Prebiotic	0:8	arg1	Mannan-Oligosaccharides					10:32	Prebiotic Mannan-Oligosaccharides	0:32	Prebiotic Mannan-Oligosaccharides	0:32	Prebiotic Mannan-Oligosaccharides Augment the Hypoglycemic Effects of Metformin in Correlation with Modulating Gut Microbiota.
29701959	5	22	theme	microbiota	1132:1141	arg1	modulation					1143:1152	gut microbiota modulation	1128:1152	gut microbiota modulation	1128:1152	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	4	23	theme	gut	771:773	arg1	composition					785:795	the gut community composition	767:795	the gut community composition	767:795	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	2	24	theme	konjac	389:394	arg1	MOS					421:423	MOS	421:423	MOS	421:423	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	2	24	theme	konjac	389:394	arg1	mannan-oligosaccharides					396:418	konjac mannan-oligosaccharides	389:418	konjac mannan-oligosaccharides (MOS)	389:424	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	3	25	theme	insulin	650:656	arg1	resistance					658:667	insulin resistance	650:667	insulin resistance	650:667	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	4	26	theme	Bifidobacterium	980:994	arg1	pseudolongum					996:1007	Bifidobacterium pseudolongum	980:1007	Bifidobacterium pseudolongum	980:1007	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	2	27	theme	intestinal	316:325	arg1	drawbacks					327:335	several intestinal drawbacks	308:335	several intestinal drawbacks	308:335	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	5	28	theme	present	1014:1020	arg1	study					1022:1026	The present study	1010:1026	The present study	1010:1026	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	2	29	with	usage	350:354	arg1	prebiotic					376:384	a prebiotic	374:384	a prebiotic of konjac mannan-oligosaccharides (MOS)	374:424	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	2	30	theme	several	308:314	arg1	drawbacks					327:335	several intestinal drawbacks	308:335	several intestinal drawbacks	308:335	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	5	31	theme	metformin	1098:1106	arg1	effects					1087:1093	the hypoglycemic effects	1070:1093	the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication	1070:1207	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	2	32	contain	has	304:306	arg1	medicine					282:289	the first line clinical medicine	258:289	the first line clinical medicine of metformin	258:302	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	2	32	contain	has	304:306	arg2	drawbacks					327:335	several intestinal drawbacks	308:335	several intestinal drawbacks	308:335	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	0	33	theme	Hypoglycemic	46:57	arg1	Effects					59:65	the Hypoglycemic Effects	42:65	the Hypoglycemic Effects of Metformin in Correlation	42:93	Prebiotic Mannan-Oligosaccharides Augment the Hypoglycemic Effects of Metformin in Correlation with Modulating Gut Microbiota.
29701959	4	34	theme	pseudolongum	996:1007	arg1	OTU05945					925:932	an unnamed OTU05945	914:932	an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum	914:1007	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	4	35	theme	Rikenellaceae	859:871	arg1	abundances					838:847	the relative abundances	825:847	the relative abundances of family Rikenellaceae and order Clostridiales	825:895	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	1	36	theme	gut	219:221	arg1	dysbacteriosis					234:247	gut microbiota dysbacteriosis	219:247	gut microbiota dysbacteriosis	219:247	Type 2 diabetes (T2D) induced by obesity and high-fat diet is significantly associated with gut microbiota dysbacteriosis.
29701959	0	37	theme	Metformin	70:78	arg1	Effects					59:65	the Hypoglycemic Effects	42:65	the Hypoglycemic Effects of Metformin in Correlation	42:93	Prebiotic Mannan-Oligosaccharides Augment the Hypoglycemic Effects of Metformin in Correlation with Modulating Gut Microbiota.
29701959	4	38	theme	Akkermansia	951:961	arg1	muciniphila					963:973	Akkermansia muciniphila	951:973	Akkermansia muciniphila	951:973	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	3	39	theme	synergistic	614:624	arg1	effects					626:632	synergistic effects	614:632	synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology	614:736	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	2	40	theme	metformin	359:367	arg1	usage					350:354	combination usage	338:354	combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS)	338:424	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	4	41	theme	order	877:881	arg1	Clostridiales					883:895	order Clostridiales	877:895	order Clostridiales	877:895	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	5	42	from	effects	1087:1093	arg1	association					1111:1121	association	1111:1121	association with gut microbiota modulation, which could provide references for further medication	1111:1207	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	3	43	theme	hepatic	720:726	arg1	histology					728:736	islet and hepatic histology	710:736	histology	728:736	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	2	44	theme	clinical	273:280	arg1	medicine					282:289	the first line clinical medicine	258:289	the first line clinical medicine of metformin	258:302	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	3	45	theme	metformin	583:591	arg1	treatment					570:578	Composite treatment	560:578	Composite treatment of metformin and MOS	560:599	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	1	46	theme	microbiota	223:232	arg1	dysbacteriosis					234:247	gut microbiota dysbacteriosis	219:247	gut microbiota dysbacteriosis	219:247	Type 2 diabetes (T2D) induced by obesity and high-fat diet is significantly associated with gut microbiota dysbacteriosis.
29701959	4	47	theme	muciniphila	963:973	arg1	OTU05945					925:932	an unnamed OTU05945	914:932	an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum	914:1007	In addition, MF+MOS altered the gut community composition and structure by decreasing the relative abundances of family Rikenellaceae and order Clostridiales while increasing an unnamed OTU05945 of family S24-7, Akkermansia muciniphila, and Bifidobacterium pseudolongum.
29701959	5	48	theme	gut	1128:1130	arg1	modulation					1143:1152	gut microbiota modulation	1128:1152	gut microbiota modulation	1128:1152	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29701959	2	49	theme	line	268:271	arg1	medicine					282:289	the first line clinical medicine	258:289	the first line clinical medicine of metformin	258:302	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	2	50	theme	combination	338:348	arg1	usage					350:354	combination usage	338:354	combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS)	338:424	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	3	51	theme	islet	710:714	arg1	histology					728:736	islet and hepatic histology	710:736	histology	728:736	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	2	52	theme	first	262:266	arg1	medicine					282:289	the first line clinical medicine	258:289	the first line clinical medicine of metformin	258:302	Because the first line clinical medicine of metformin has several intestinal drawbacks, combination usage of metformin with a prebiotic of konjac mannan-oligosaccharides (MOS) was conceived and implemented aiming to investigate whether there were some intestinal synergetic effects and how MOS would function.
29701959	3	53	dep	effects	626:632	arg1	ameliorating					637:648	ameliorating	637:648	ameliorating insulin resistance and glucose tolerance	637:689	Composite treatment of metformin and MOS demonstrated synergistic effects on ameliorating insulin resistance and glucose tolerance, also on repairing islet and hepatic histology.
29701959	5	54	theme	hypoglycemic	1074:1085	arg1	effects					1087:1093	the hypoglycemic effects	1070:1093	the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication	1070:1207	The present study suggested that usage of MOS could augment the hypoglycemic effects of metformin in association with gut microbiota modulation, which could provide references for further medication.
29693569	0	0	theme	Subsequent	73:82	arg1	Degradation					84:94	Subsequent Degradation	73:94	Subsequent Degradation	73:94	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	0	1	with	Biohybrid	35:43	arg1	Cellulose					59:67	Cellulose	59:67	Cellulose	59:67	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	10	2	theme	cellulose-CuHARS	1064:1079	arg1	films					1091:1095	The cellulose-CuHARS composite films	1060:1095	The cellulose-CuHARS composite films	1060:1095	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	7	3	theme	complete	680:687	arg1	media					702:706	complete cell culture media	680:706	complete cell culture media	680:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	10	4	theme	usable	1141:1146	arg1	pieces					1148:1153	usable pieces	1141:1153	usable pieces	1141:1153	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	2	5	theme	cellular	328:335	arg1	media					337:341	cellular media	328:341	cellular media	328:341	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	7	6	theme	cell	689:692	arg1	media					702:706	complete cell culture media	680:706	complete cell culture media	680:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	8	7	theme	aqueous	927:933	arg1	phase					950:954	an aqueous (pulp mixture) phase	924:954	an aqueous (pulp mixture) phase	924:954	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	8	7	theme	aqueous	927:933	arg1	mixture					941:947	pulp mixture	936:947	pulp mixture	936:947	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	8	8	theme	CuHARS	806:811	arg1	integration					791:801	Stable integration	784:801	Stable integration of CuHARS into cellulose matrices	784:835	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	7	9	theme	CuHARS	670:675	arg1	g/mL					658:661	25 &mu;g/mL	651:661	25 &mu;g/mL of the CuHARS in complete cell culture media	651:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	7	10	from	g/mL	658:661	arg1	media					702:706	complete cell culture media	680:706	complete cell culture media	680:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	7	11	dep	degraded	723:730	arg1	slowly					733:738	slowly	733:738	slowly	733:738	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	7	12	theme	physiological	758:770	arg1	conditions					772:781	physiological conditions	758:781	physiological conditions	758:781	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	11	13	theme	2D/3D	1478:1482	arg1	matrix					1484:1489	a 2D/3D matrix	1476:1489	a 2D/3D matrix using natural products (cellulose)	1476:1524	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	11	14	dep	platform	1418:1425	arg1	1					1413:1413	1	1413:1413	1	1413:1413	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	10	15	with	compatible	1239:1248	arg1	studies					1268:1274	cell culture studies	1255:1274	cell culture studies using brain tumor (glioma) cells	1255:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	2	16	from	degradable	314:323	arg1	media					337:341	cellular media	328:341	cellular media	328:341	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	10	17	from	rehydration	1189:1199	arg1	water					1204:1208	water	1204:1208	water	1204:1208	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	8	18	theme	cellulose	818:826	arg1	matrices					828:835	cellulose matrices	818:835	cellulose matrices	818:835	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	0	19	theme	Biomedical	100:109	arg1	Control					111:117	Biomedical Control	100:117	Biomedical Control	100:117	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	4	20	theme	Synthesized	465:475	arg1	CuHARS					477:482	Synthesized CuHARS	465:482	Synthesized CuHARS	465:482	Synthesized CuHARS was concentrated by centrifugation and then dried.
29693569	2	21	from	media	337:341	arg1	degradable					314:323	degradable	314:323	degradable	314:323	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	1	22	theme	material	223:230	arg1	synthesis					154:162	the novel synthesis	144:162	the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine	144:244	We previously described the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine.
29693569	10	23	theme	tumor	1288:1292	arg1	cells					1303:1307	brain tumor (glioma) cells	1282:1307	brain tumor (glioma) cells	1282:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	11	24	theme	applied	1380:1386	arg1	integration					1459:1469	(2) integration	1455:1469	(2) integration into a 2D/3D matrix using natural products (cellulose)	1455:1524	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	11	24	theme	applied	1380:1386	arg1	platform					1418:1425	a platform	1416:1425	: (1) a platform for biomedical tracking	1410:1449	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	11	24	theme	applied	1380:1386	arg1	applications					1388:1399	applied applications	1380:1399	applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose)	1380:1524	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	11	25	theme	biohybrid	1366:1374	arg1	utility					1336:1342	utility	1336:1342	utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose)	1336:1524	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	9	26	theme	susceptible	1047:1057	arg1	hybrids					1026:1032	the hybrids	1022:1032	the hybrids magnetically susceptible	1022:1057	Additional materials were integrated to make the hybrids magnetically susceptible.
29693569	0	27	theme	Copper-Containing	17:33	arg1	CuHARS					46:51	CuHARS	46:51	CuHARS	46:51	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	0	27	theme	Copper-Containing	17:33	arg1	Biohybrid					35:43	a Copper-Containing Biohybrid	15:43	a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control	15:117	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	7	28	from	CuHARS	670:675	arg1	media					702:706	complete cell culture media	680:706	complete cell culture media	680:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	8	29	theme	pulp	936:939	arg1	phase					950:954	an aqueous (pulp mixture) phase	924:954	an aqueous (pulp mixture) phase	924:954	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	8	29	theme	pulp	936:939	arg1	mixture					941:947	pulp mixture	936:947	pulp mixture	936:947	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	11	30	theme	CuHARS-cellulose	1349:1364	arg1	biohybrid					1366:1374	a CuHARS-cellulose biohybrid	1347:1374	a CuHARS-cellulose biohybrid	1347:1374	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	2	31	from	stable	263:268	arg1	water					273:277	water	273:277	water	273:277	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	10	32	theme	cell	1255:1258	arg1	studies					1268:1274	cell culture studies	1255:1274	cell culture studies using brain tumor (glioma) cells	1255:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	3	33	theme	cellulose	381:389	arg1	matrices					391:398	cellulose matrices	381:398	cellulose matrices	381:398	Here, integration of the CuHARS into cellulose matrices was carried out to provide added control for CuHARS degradation.
29693569	10	34	theme	composite	1081:1089	arg1	films					1091:1095	The cellulose-CuHARS composite films	1060:1095	The cellulose-CuHARS composite films	1060:1095	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	11	35	dep	integration	1459:1469	arg1	2					1456:1456	2	1456:1456	2	1456:1456	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	0	36	theme	Biohybrid	35:43	arg1	Integration					0:10	Integration	0:10	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.	0:118	Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical Control.
29693569	6	37	from	stable	590:595	arg1	water					600:604	water	600:604	water	600:604	CuHARS was stable in water for months without degradation.
29693569	10	38	theme	brain	1282:1286	arg1	cells					1303:1307	brain tumor (glioma) cells	1282:1307	brain tumor (glioma) cells	1282:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	3	39	theme	added	427:431	arg1	control					433:439	added control	427:439	added control	427:439	Here, integration of the CuHARS into cellulose matrices was carried out to provide added control for CuHARS degradation.
29693569	3	40	theme	CuHARS	369:374	arg1	integration					350:360	integration	350:360	integration of the CuHARS into cellulose matrices	350:398	Here, integration of the CuHARS into cellulose matrices was carried out to provide added control for CuHARS degradation.
29693569	4	41	dep	concentrated	488:499	arg1	then					523:526	then	523:526	then	523:526	Synthesized CuHARS was concentrated by centrifugation and then dried.
29693569	4	41	dep	concentrated	488:499	arg1	centrifugation					504:517	centrifugation	504:517	centrifugation	504:517	Synthesized CuHARS was concentrated by centrifugation and then dried.
29693569	4	41	dep	concentrated	488:499	arg1	dried					528:532	dried	528:532	dried	528:532	Synthesized CuHARS was concentrated by centrifugation and then dried.
29693569	8	42	theme	cellulose	877:885	arg1	micro-					887:892	cellulose micro-	877:892	cellulose micro-	877:892	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	2	43	dep	completely	290:299	arg1	but					302:304	but	302:304	but	302:304	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	7	44	from	media	702:706	arg1	g/mL					658:661	25 &mu;g/mL	651:661	25 &mu;g/mL of the CuHARS in complete cell culture media	651:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	5	45	theme	weighed	539:545	arg1	mass					547:550	The weighed mass	535:550	The weighed mass	535:550	The weighed mass was re-suspended in water.
29693569	2	46	from	water	273:277	arg1	stable					263:268	stable	263:268	stable	263:268	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	7	47	theme	&mu	654:656	arg1	g/mL					658:661	25 &mu;g/mL	651:661	25 &mu;g/mL of the CuHARS in complete cell culture media	651:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	6	48	from	water	600:604	arg1	stable					590:595	stable	590:595	stable	590:595	CuHARS was stable in water for months without degradation.
29693569	10	49	theme	culture	1260:1266	arg1	studies					1268:1274	cell culture studies	1255:1274	cell culture studies using brain tumor (glioma) cells	1255:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	2	50	dep	but	302:304	arg1	slowly					306:311	slowly	306:311	slowly	306:311	While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media.
29693569	1	51	theme	biohybrid	213:221	arg1	material					223:230	a copper high-aspect ratio structure (CuHARS) biohybrid material	167:230	a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine	167:244	We previously described the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine.
29693569	11	52	theme	natural	1497:1503	arg1	products					1505:1512	natural products	1497:1512	natural products (cellulose)	1497:1524	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	11	52	theme	natural	1497:1503	arg1	cellulose					1515:1523	cellulose	1515:1523	cellulose	1515:1523	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	8	53	theme	Stable	784:789	arg1	integration					791:801	Stable integration	784:801	Stable integration of CuHARS into cellulose matrices	784:835	Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying.
29693569	3	54	theme	CuHARS	445:450	arg1	degradation					452:462	CuHARS degradation	445:462	CuHARS degradation	445:462	Here, integration of the CuHARS into cellulose matrices was carried out to provide added control for CuHARS degradation.
29693569	11	55	theme	biomedical	1431:1440	arg1	tracking					1442:1449	biomedical tracking	1431:1449	biomedical tracking	1431:1449	These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
29693569	10	56	theme	glioma	1295:1300	arg1	cells					1303:1307	brain tumor (glioma) cells	1282:1307	brain tumor (glioma) cells	1282:1307	The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells.
29693569	9	57	theme	Additional	977:986	arg1	materials					988:996	Additional materials	977:996	Additional materials	977:996	Additional materials were integrated to make the hybrids magnetically susceptible.
29693569	7	58	theme	culture	694:700	arg1	media					702:706	complete cell culture media	680:706	complete cell culture media	680:706	In contrast, 25 &mu;g/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions.
29693569	1	59	theme	novel	148:152	arg1	synthesis					154:162	the novel synthesis	144:162	the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine	144:244	We previously described the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine.
31685836	8	0	theme	antimicrobial	1418:1430	arg1	activity					1432:1439	an excellent antimicrobial activity	1405:1439	an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi	1405:1525	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	0	1	with	Hydrogel	49:56	arg1	Properties					97:106	Tunable Mechanical and Biological Properties	63:106	Tunable Mechanical and Biological Properties	63:106	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	2	2	theme	tissue	389:394	arg1	character					367:375	an analogous character	354:375	an analogous character of the soft tissue	354:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	8	3	theme	cartilage	1653:1661	arg1	regeneration					1663:1674	cartilage regeneration	1653:1674	cartilage regeneration	1653:1674	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	6	4	theme	self-recoverable	1171:1186	arg1	behavior					1210:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior	1149:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test	1149:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	1	5	theme	cartilage	189:197	arg1	regeneration					199:210	cartilage regeneration	189:210	cartilage regeneration with superior mechanobiological properties	189:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	8	6	theme	composite	1630:1638	arg1	hydrogel					1640:1647	a composite hydrogel	1628:1647	a composite hydrogel for cartilage regeneration	1628:1674	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	2	7	theme	implant	329:335	arg1	materials					337:345	the promising implant materials	315:345	the promising implant materials due to an analogous character of the soft tissue	315:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	2	7	theme	implant	329:335	arg1	hydrogels					301:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	3	8	theme	chitosan	594:601	arg1	hydrogel					603:610	a composite chitosan hydrogel	582:610	a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr)	582:657	This can be solved by developing a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr).
31685836	6	9	theme	compression	1049:1059	arg1	strength					1061:1068	The compression strength	1045:1068	The compression strength	1045:1068	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	0	10	theme	Cartilage	112:120	arg1	Regeneration					122:133	Cartilage Regeneration	112:133	Cartilage Regeneration	112:133	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	5	11	from	concentration	865:877	arg1	hydrogel					914:921	the composite hydrogel	900:921	the composite hydrogel	900:921	The concentration of hydroxyapatite in the composite hydrogel was optimized and it was found that, reinforcement modifies the hydrogel network by promoting the secondary crosslinking.
31685836	5	12	theme	secondary	1021:1029	arg1	crosslinking					1031:1042	the secondary crosslinking	1017:1042	the secondary crosslinking	1017:1042	The concentration of hydroxyapatite in the composite hydrogel was optimized and it was found that, reinforcement modifies the hydrogel network by promoting the secondary crosslinking.
31685836	2	13	theme	promising	319:327	arg1	materials					337:345	the promising implant materials	315:345	the promising implant materials due to an analogous character of the soft tissue	315:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	2	13	theme	promising	319:327	arg1	hydrogels					301:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	7	14	theme	proposed	1349:1356	arg1	application					1358:1368	the proposed application	1345:1368	the proposed application	1345:1368	The storage modulus value can reach nearly 10 kPa which is needed for the proposed application.
31685836	7	15	theme	storage	1279:1285	arg1	value					1295:1299	The storage modulus value	1275:1299	The storage modulus value	1275:1299	The storage modulus value can reach nearly 10 kPa which is needed for the proposed application.
31685836	6	16	theme	time-dependent	1149:1162	arg1	behavior					1210:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior	1149:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test	1149:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	4	17	theme	different	810:818	arg1	concentration					820:832	different concentration	810:832	different concentration of hydroxyapatite nanorod	810:858	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	2	18	theme	mechanical	416:425	arg1	strength					427:434	their low mechanical strength	406:434	their low mechanical strength	406:434	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	2	19	theme	integrity	477:485	arg1	lack					469:472	its lack	465:472	its lack of integrity with surrounding tissues	465:510	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	6	20	theme	compression	1257:1267	arg1	test					1269:1272	the cyclic loading-unloading compression test	1228:1272	the cyclic loading-unloading compression test	1228:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	2	21	theme	low	412:414	arg1	strength					427:434	their low mechanical strength	406:434	their low mechanical strength	406:434	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	6	22	theme	loading-unloading	1239:1255	arg1	test					1269:1272	the cyclic loading-unloading compression test	1228:1272	the cyclic loading-unloading compression test	1228:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	5	23	theme	hydrogel	987:994	arg1	network					996:1002	the hydrogel network	983:1002	the hydrogel network	983:1002	The concentration of hydroxyapatite in the composite hydrogel was optimized and it was found that, reinforcement modifies the hydrogel network by promoting the secondary crosslinking.
31685836	1	24	theme	superior	217:224	arg1	properties					244:253	superior mechanobiological properties	217:253	superior mechanobiological properties	217:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	6	25	theme	cyclic	1232:1237	arg1	test					1269:1272	the cyclic loading-unloading compression test	1228:1272	the cyclic loading-unloading compression test	1228:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	1	26	theme	mechanobiological	226:242	arg1	properties					244:253	superior mechanobiological properties	217:253	superior mechanobiological properties	217:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	0	27	theme	Composite	39:47	arg1	Hydrogel					49:56	Chitosan Composite Hydrogel	30:56	Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration	30:133	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	8	28	theme	composite	1380:1388	arg1	hydrogels					1390:1398	composite hydrogels	1380:1398	composite hydrogels	1380:1398	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	8	29	dep	show	1400:1403	arg1	provide					1586:1592	provide	1586:1592	show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration	1400:1674	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	2	30	theme	due	347:349	arg1	materials					337:345	the promising implant materials	315:345	the promising implant materials due to an analogous character of the soft tissue	315:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	2	30	theme	due	347:349	arg1	hydrogels					301:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	8	31	theme	Candida	1504:1510	arg1	albicans					1512:1519	Candida albicans	1504:1519	Candida albicans	1504:1519	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	5	32	theme	hydroxyapatite	882:895	arg1	concentration					865:877	The concentration	861:877	The concentration of hydroxyapatite in the composite hydrogel	861:921	The concentration of hydroxyapatite in the composite hydrogel was optimized and it was found that, reinforcement modifies the hydrogel network by promoting the secondary crosslinking.
31685836	0	33	theme	Chitosan	30:37	arg1	Hydrogel					49:56	Chitosan Composite Hydrogel	30:56	Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration	30:133	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	8	34	theme	mouse	1568:1572	arg1	fibroblasts					1574:1584	L929 mouse fibroblasts	1563:1584	L929 mouse fibroblasts	1563:1584	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	2	35	theme	soft	384:387	arg1	tissue					389:394	the soft tissue	380:394	the soft tissue	380:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	8	36	theme	an excellent	1405:1416	arg1	activity					1432:1439	an excellent antimicrobial activity	1405:1439	an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi	1405:1525	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	8	37	theme	L929	1563:1566	arg1	fibroblasts					1574:1584	L929 mouse fibroblasts	1563:1584	L929 mouse fibroblasts	1563:1584	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	2	38	theme	Chitosan	287:294	arg1	materials					337:345	the promising implant materials	315:345	the promising implant materials due to an analogous character of the soft tissue	315:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	2	38	theme	Chitosan	287:294	arg1	hydrogels					301:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels	287:309	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	6	39	theme	%	1134:1134	arg1	deformation					1117:1127	a significant deformation	1103:1127	a significant deformation of 32%	1103:1134	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	4	40	theme	work	682:685	arg1	objective					664:672	The objective	660:672	The objective of this work	660:685	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	2	41	theme	surrounding	492:502	arg1	tissues					504:510	surrounding tissues	492:510	surrounding tissues	492:510	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	8	42	dep	coli	1461:1464	arg1	fungi					1521:1525	fungi	1521:1525	fungi	1521:1525	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	4	43	theme	composite	778:786	arg1	hydrogels					788:796	the composite hydrogels	774:796	the composite hydrogels loaded with different concentration of hydroxyapatite nanorod	774:858	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	0	44	theme	Mechanical	71:80	arg1	Properties					97:106	Tunable Mechanical and Biological Properties	63:106	Tunable Mechanical and Biological Properties	63:106	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	6	45	dep	time-dependent	1149:1162	arg1	rapid					1165:1169	rapid	1165:1169	rapid	1165:1169	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	6	46	theme	significant	1105:1115	arg1	deformation					1117:1127	a significant deformation	1103:1127	a significant deformation of 32%	1103:1134	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	2	47	dep	together	451:458	arg1	with					460:463	with	460:463	with	460:463	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	0	48	theme	Tunable	63:69	arg1	Properties					97:106	Tunable Mechanical and Biological Properties	63:106	Tunable Mechanical and Biological Properties	63:106	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	7	49	theme	modulus	1287:1293	arg1	value					1295:1299	The storage modulus value	1275:1299	The storage modulus value	1275:1299	The storage modulus value can reach nearly 10 kPa which is needed for the proposed application.
31685836	8	50	theme	potential	1596:1604	arg1	pathway					1606:1612	a potential pathway	1594:1612	a potential pathway to developing a composite hydrogel for cartilage regeneration	1594:1674	Besides, composite hydrogels show an excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus bacteria's and Candida albicans fungi and their cytocompatibility towards L929 mouse fibroblasts provide a potential pathway to developing a composite hydrogel for cartilage regeneration.
31685836	3	51	theme	Hydroxyapatite	628:641	arg1	Nanorods					643:650	Hydroxyapatite Nanorods	628:650	Hydroxyapatite Nanorods (HANr)	628:657	This can be solved by developing a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr).
31685836	3	51	theme	Hydroxyapatite	628:641	arg1	HANr					653:656	HANr	653:656	HANr	653:656	This can be solved by developing a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr).
31685836	1	52	theme	continuous	145:154	arg1	quest					156:160	the continuous quest	141:160	the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties	141:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	1	53	with	regeneration	199:210	arg1	properties					244:253	superior mechanobiological properties	217:253	superior mechanobiological properties	217:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	2	54	with	lack	469:472	arg1	tissues					504:510	surrounding tissues	492:510	surrounding tissues	492:510	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	6	55	theme	resistant	1200:1208	arg1	behavior					1210:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior	1149:1217	time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test	1149:1272	The compression strength could reach 1.62 ± 0.02 MPa with a significant deformation of 32% and exhibits time-dependent, rapid self-recoverable and fatigue resistant behavior based on the cyclic loading-unloading compression test.
31685836	3	56	theme	composite	584:592	arg1	hydrogel					603:610	a composite chitosan hydrogel	582:610	a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr)	582:657	This can be solved by developing a composite chitosan hydrogel reinforced with Hydroxyapatite Nanorods (HANr).
31685836	4	57	dep	develop	693:699	arg1	and					756:758	and	756:758	and	756:758	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	2	58	theme	analogous	357:365	arg1	character					367:375	an analogous character	354:375	an analogous character of the soft tissue	354:394	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	0	59	theme	Biological	86:95	arg1	Properties					97:106	Tunable Mechanical and Biological Properties	63:106	Tunable Mechanical and Biological Properties	63:106	Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.
31685836	5	60	theme	composite	904:912	arg1	hydrogel					914:921	the composite hydrogel	900:921	the composite hydrogel	900:921	The concentration of hydroxyapatite in the composite hydrogel was optimized and it was found that, reinforcement modifies the hydrogel network by promoting the secondary crosslinking.
31685836	4	61	theme	nanorod	852:858	arg1	concentration					820:832	different concentration	810:832	different concentration of hydroxyapatite nanorod	810:858	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	1	62	theme	developing	165:174	arg1	hydrogel					176:183	developing hydrogel	165:183	developing hydrogel	165:183	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31685836	2	63	theme	load-bearing	523:534	arg1	application					536:546	the load-bearing application	519:546	the load-bearing application	519:546	Chitosan (CS) hydrogels are the promising implant materials due to an analogous character of the soft tissue; however, their low mechanical strength and durability together with its lack of integrity with surrounding tissues hinder the load-bearing application.
31685836	4	64	theme	hydroxyapatite	837:850	arg1	nanorod					852:858	hydroxyapatite nanorod	837:858	hydroxyapatite nanorod	837:858	The objective of this work is to develop and characterize (physically, chemically, mechanically and biologically) the composite hydrogels loaded with different concentration of hydroxyapatite nanorod.
31685836	1	65	theme	hydrogel	176:183	arg1	quest					156:160	the continuous quest	141:160	the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties	141:253	With the continuous quest of developing hydrogel for cartilage regeneration with superior mechanobiological properties are still becoming a challenge.
31152840	0	0	theme	cellulose	75:83	arg1	acetate					85:91	cellulose acetate	75:91	cellulose acetate	75:91	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.
31152840	6	1	theme	FTIR	888:891	arg1	spectroscopy					893:904	FTIR spectroscopy	888:904	FTIR spectroscopy	888:904	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	1	2	theme	sorghum	148:154	arg1	straw					156:160	sorghum straw	148:160	sorghum straw	148:160	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	6	3	with	acetate	989:995	arg1	degrees					1008:1014	2.62 degrees	1003:1014	2.62 degrees of substitution	1003:1030	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	6	4	theme	cellulose	979:987	arg1	acetate					989:995	cellulose acetate	979:995	cellulose acetate (with 2.62 degrees of substitution)	979:1031	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	0	5	theme	acetate	85:91	arg1	synthesis					62:70	synthesis	62:70	synthesis	62:70	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.
31152840	0	5	theme	acetate	85:91	arg1	optimization					45:56	optimization	45:56	optimization	45:56	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.
31152840	5	6	from	80 °C	734:738	arg1	concentration					669:681	dilute alkali concentration	655:681	dilute alkali concentration	655:681	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	6	from	80 °C	734:738	arg1	conditions					592:601	The optimum conditions	580:601	The optimum conditions that maximize these processes	580:631	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	6	from	80 °C	734:738	arg1	35 min					698:703	35 min	698:703	35 min	698:703	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	6	from	80 °C	734:738	arg1	25 mL					709:713	25 mL	709:713	25 mL	709:713	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	6	from	80 °C	734:738	arg1	%					652:652	2.5 h and 1.25%	638:652	2.5 h and 1.25% (dilute alkali concentration) at 90 °C	638:691	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	6	from	80 °C	734:738	arg1	volume					723:728	bleach volume	716:728	bleach volume	716:728	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	1	7	dep	pulping	123:129	arg1	the					119:121	the	119:121	the	119:121	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	4	8	theme	sorghum	489:495	arg1	straw					497:501	The sorghum straw	485:501	The sorghum straw	485:501	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	1	9	theme	straw	156:160	arg1	bleaching					135:143	bleaching	135:143	bleaching	135:143	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	1	9	theme	straw	156:160	arg1	pulping					123:129	pulping	123:129	pulping	123:129	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	6	10	theme	bleached	1048:1055	arg1	pulp					1057:1060	bleached pulp	1048:1060	bleached pulp for 16 h at 25 °C	1048:1078	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	3	11	dep	35 min	426:431	arg1	to					423:424	to	423:424	to	423:424	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	4	12	theme	%	545:545	arg1	hemicellulose					547:559	19.18% hemicellulose	540:559	19.18% hemicellulose	540:559	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	3	13	theme	alkali	347:352	arg1	%					381:381	0.75 to 1.25%	369:381	0.75 to 1.25%	369:381	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	3	13	theme	alkali	347:352	arg1	concentration					354:366	dilute alkali concentration	340:366	dilute alkali concentration (0.75 to 1.25%)	340:382	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	5	14	from	90 °C	687:691	arg1	concentration					669:681	dilute alkali concentration	655:681	dilute alkali concentration	655:681	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	14	from	90 °C	687:691	arg1	conditions					592:601	The optimum conditions	580:601	The optimum conditions that maximize these processes	580:631	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	14	from	90 °C	687:691	arg1	35 min					698:703	35 min	698:703	35 min	698:703	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	14	from	90 °C	687:691	arg1	25 mL					709:713	25 mL	709:713	25 mL	709:713	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	14	from	90 °C	687:691	arg1	%					652:652	2.5 h and 1.25%	638:652	2.5 h and 1.25% (dilute alkali concentration) at 90 °C	638:691	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	14	from	90 °C	687:691	arg1	volume					723:728	bleach volume	716:728	bleach volume	716:728	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	6	15	from	25 °C	1074:1078	arg1	pulp					1057:1060	bleached pulp	1048:1060	bleached pulp for 16 h at 25 °C	1048:1078	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	5	16	theme	low	778:780	arg1	number					788:793	a low Kappa number	776:793	a low Kappa number (<3)	776:798	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	16	theme	low	778:780	arg1	<3					796:797	<3	796:797	<3	796:797	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	4	17	theme	30.42	565:569	arg1	%					570:570	%	570:570	%	570:570	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	0	18	theme	Sorghum	0:6	arg1	straw					8:12	Sorghum straw	0:12	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.	0:92	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.
31152840	3	19	dep	1.25	377:380	arg1	to					374:375	to	374:375	to	374:375	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	5	20	theme	bleach	716:721	arg1	volume					723:728	bleach volume	716:728	bleach volume	716:728	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	20	theme	bleach	716:721	arg1	35 min					698:703	35 min	698:703	35 min	698:703	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	21	theme	Kappa	782:786	arg1	number					788:793	a low Kappa number	776:793	a low Kappa number (<3)	776:798	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	21	theme	Kappa	782:786	arg1	<3					796:797	<3	796:797	<3	796:797	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	6	22	theme	1H	875:876	arg1	NMR					878:880	The 1H NMR	871:880	The 1H NMR	871:880	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	0	23	dep	straw	8:12	arg1	process					37:43	process	37:43	process optimization and synthesis of cellulose acetate	37:91	Sorghum straw: Pulping and bleaching process optimization and synthesis of cellulose acetate.
31152840	5	24	theme	optimum	584:590	arg1	conditions					592:601	The optimum conditions	580:601	The optimum conditions that maximize these processes	580:631	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	24	theme	optimum	584:590	arg1	%					652:652	2.5 h and 1.25%	638:652	2.5 h and 1.25% (dilute alkali concentration) at 90 °C	638:691	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	3	25	theme	dilute	340:345	arg1	%					381:381	0.75 to 1.25%	369:381	0.75 to 1.25%	369:381	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	3	25	theme	dilute	340:345	arg1	concentration					354:366	dilute alkali concentration	340:366	dilute alkali concentration (0.75 to 1.25%)	340:382	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	4	26	theme	%	525:525	arg1	α-cellulose					527:537	49.43% α-cellulose	520:537	49.43% α-cellulose	520:537	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	5	27	theme	dilute	655:660	arg1	concentration					669:681	dilute alkali concentration	655:681	dilute alkali concentration	655:681	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	27	theme	dilute	655:660	arg1	%					652:652	2.5 h and 1.25%	638:652	2.5 h and 1.25% (dilute alkali concentration) at 90 °C	638:691	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	4	28	theme	19.18	540:544	arg1	%					545:545	%	545:545	%	545:545	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	5	29	theme	alkali	662:667	arg1	concentration					669:681	dilute alkali concentration	655:681	dilute alkali concentration	655:681	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	29	theme	alkali	662:667	arg1	%					652:652	2.5 h and 1.25%	638:652	2.5 h and 1.25% (dilute alkali concentration) at 90 °C	638:691	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	6	30	theme	substitution	1019:1030	arg1	degrees					1008:1014	2.62 degrees	1003:1014	2.62 degrees of substitution	1003:1030	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	5	31	theme	high	841:844	arg1	degree					846:851	a high degree	839:851	a high degree of crystallinity	839:868	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	32	with	pulps	765:769	arg1	number					788:793	a low Kappa number	776:793	a low Kappa number (<3)	776:798	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	32	with	pulps	765:769	arg1	content					811:817	lignin content	804:817	lignin content	804:817	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	32	with	pulps	765:769	arg1	<3					796:797	<3	796:797	<3	796:797	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	6	33	theme	thermal	915:921	arg1	analysis					923:930	thermal analysis	915:930	thermal analysis	915:930	The 1H NMR, XRD, FTIR spectroscopy, SEM and thermal analysis demonstrated that it is possible to synthetize cellulose acetate (with 2.62 degrees of substitution) by acetylating bleached pulp for 16 h at 25 °C.
31152840	3	34	dep	2.5 h	329:333	arg1	to					326:327	to	326:327	to	326:327	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	5	35	theme	crystallinity	856:868	arg1	degree					846:851	a high degree	839:851	a high degree of crystallinity	839:868	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	2	36	theme	composite	244:252	arg1	design					254:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design was carried out.
31152840	1	37	theme	cellulose	202:210	arg1	acetate					212:218	cellulose acetate	202:218	cellulose acetate	202:218	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	5	38	theme	lignin	804:809	arg1	content					811:817	lignin content	804:817	lignin content	804:817	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	2	39	theme	central	236:242	arg1	design					254:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design was carried out.
31152840	3	40	dep	25 mL	459:463	arg1	to					456:457	to	456:457	to	456:457	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	1	41	theme	acetate	212:218	arg1	synthesis					189:197	the synthesis	185:197	the synthesis of cellulose acetate	185:218	The process employed for the pulping and bleaching of sorghum straw was optimized prior to the synthesis of cellulose acetate.
31152840	2	42	theme	factorial	226:234	arg1	design					254:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design	221:259	A 22 factorial central composite design was carried out.
31152840	3	43	theme	bleach	438:443	arg1	25 mL					459:463	20 to 25 mL	453:463	20 to 25 mL	453:463	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	3	43	theme	bleach	438:443	arg1	volume					445:450	bleach volume	438:450	bleach volume (20 to 25 mL)	438:464	The variables considered were cooking time (1.5 to 2.5 h) and dilute alkali concentration (0.75 to 1.25%) for the pulping and bleaching time (30 to 35 min) and bleach volume (20 to 25 mL) for the bleaching.
31152840	4	44	theme	49.43	520:524	arg1	%					525:525	%	525:525	%	525:525	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31152840	5	45	with	cellulose	824:832	arg1	number					788:793	a low Kappa number	776:793	a low Kappa number (<3)	776:798	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	45	with	cellulose	824:832	arg1	content					811:817	lignin content	804:817	lignin content	804:817	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	5	45	with	cellulose	824:832	arg1	<3					796:797	<3	796:797	<3	796:797	The optimum conditions that maximize these processes were 2.5 h and 1.25% (dilute alkali concentration) at 90 °C, and 35 min and 25 mL (bleach volume) at 80 °C, respectively, providing pulps with a low Kappa number (<3) and lignin content, and cellulose with a high degree of crystallinity.
31152840	4	46	theme	%	570:570	arg1	lignin					572:577	30.42% lignin	565:577	30.42% lignin	565:577	The sorghum straw was comprised of 49.43% α-cellulose, 19.18% hemicellulose and 30.42% lignin.
31022483	2	0	theme	fluoroquinolone	481:495	arg1	antibiotics					497:507	a model fluoroquinolone antibiotics	473:507	a model fluoroquinolone antibiotics	473:507	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	1	1	from	wastewaters	196:206	arg1	Recovery					157:164	Recovery	157:164	Recovery of antibiotics from water and wastewaters	157:206	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	7	2	theme	hydrophobic	1182:1192	arg1	interactions					1194:1205	hydrophobic interactions	1182:1205	hydrophobic interactions	1182:1205	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	2	3	theme	model	475:479	arg1	antibiotics					497:507	a model fluoroquinolone antibiotics	473:507	a model fluoroquinolone antibiotics	473:507	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	4	4	theme	process	670:676	arg1	Modelling					629:637	Modelling	629:637	Modelling	629:637	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	4	4	theme	process	670:676	arg1	optimization					643:654	optimization	643:654	optimization	643:654	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	1	5	theme	serious	267:273	arg1	health					275:280	their serious health	261:280	their serious health	261:280	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	7	6	theme	due	1140:1142	arg1	pH 6					1135:1138	pH 6	1135:1138	pH 6 due to the electrostatic interactions and hydrophobic interactions	1135:1205	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	9	7	dep	regenerability	1344:1357	arg1	removal					1431:1437	removal	1431:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal	1339:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	1	8	from	water	186:190	arg1	Recovery					157:164	Recovery	157:164	Recovery of antibiotics from water and wastewaters	157:206	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	3	9	theme	Fe-CS	552:556	arg1	NCs					558:560	Fe-CS NCs	552:560	Fe-CS NCs	552:560	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	9	10	theme	high	1386:1389	arg1	capacity					1391:1398	its high capacity	1382:1398	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal	1339:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	0	11	theme	kinetic	109:115	arg1	Isotherm					99:106	Isotherm	99:106	Isotherm	99:106	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	0	11	theme	kinetic	109:115	arg1	studies					148:154	kinetic, thermodynamic and reusability studies	109:154	kinetic, thermodynamic and reusability studies	109:154	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	9	12	theme	wastewaters	1487:1497	arg1	remediation					1472:1482	remediation	1472:1482	remediation of wastewaters	1472:1497	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	7	13	theme	adsorption	1057:1066	arg1	rate					1068:1071	the adsorption rate	1053:1071	the adsorption rate	1053:1071	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	3	14	theme	NCs	558:560	arg1	composition					514:524	composition	514:524	composition	514:524	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	3	14	theme	NCs	558:560	arg1	morphology					538:547	surface morphology	530:547	surface morphology	530:547	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	4	15	theme	response	697:704	arg1	RSM					727:729	RSM	727:729	RSM	727:729	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	4	15	theme	response	697:704	arg1	methodology					714:724	response surface methodology	697:724	response surface methodology (RSM)	697:730	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	9	16	theme	antibiotics	1419:1429	arg1	capacity					1391:1398	its high capacity	1382:1398	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal	1339:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	0	17	theme	thermodynamic	118:130	arg1	Isotherm					99:106	Isotherm	99:106	Isotherm	99:106	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	0	17	theme	thermodynamic	118:130	arg1	studies					148:154	kinetic, thermodynamic and reusability studies	109:154	kinetic, thermodynamic and reusability studies	109:154	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	7	18	theme	chitosan	1024:1031	arg1	groups					1037:1042	the chitosan NH2 groups	1020:1042	the chitosan NH2 groups	1020:1042	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	0	19	theme	Mechanistic	0:10	arg1	investigation					12:24	Mechanistic investigation	0:24	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan	0:82	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	7	20	located	observed	989:996	arg2	rate					980:983	low adsorption rate	965:983	low adsorption rate	965:983	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	7	20	located	observed	989:996	arg1	pH < pHiep					954:963	pH < pHiep	954:963	pH < pHiep	954:963	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	9	21	theme	fluoroquinolone	1403:1417	arg1	antibiotics					1419:1429	fluoroquinolone antibiotics	1403:1429	fluoroquinolone antibiotics	1403:1429	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	7	22	theme	maximum	1118:1124	arg1	level					1126:1130	a maximum level	1116:1130	a maximum level	1116:1130	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	3	23	theme	surface	530:536	arg1	morphology					538:547	surface morphology	530:547	surface morphology	530:547	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	7	24	theme	NH2	1033:1035	arg1	groups					1037:1042	the chitosan NH2 groups	1020:1042	the chitosan NH2 groups	1020:1042	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	6	25	attach	derived	837:843	arg2	results					829:835	the results	825:835	the results derived from the models	825:859	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	6	25	attach	derived	837:843	arg1	models					854:859	the models	850:859	the models	850:859	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	2	26	theme	ciprofloxacin	449:461	arg1	removal					438:444	the adsorptive removal	423:444	the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics	423:507	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	1	27	theme	environmental	286:298	arg1	problems					300:307	environmental problems	286:307	environmental problems	286:307	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	7	28	theme	groups	1037:1042	arg1	protonation					1005:1015	protonation	1005:1015	protonation of the chitosan NH2 groups	1005:1042	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	3	29	dep	composition	514:524	arg1	The					510:512	The	510:512	The	510:512	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	9	30	dep	together	1368:1375	arg1	with					1377:1380	with	1377:1380	with	1377:1380	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	0	31	theme	ciprofloxacin	29:41	arg1	recovery					43:50	ciprofloxacin recovery	29:50	ciprofloxacin recovery	29:50	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	8	32	theme	maximum	1265:1271	arg1	capacity					1284:1291	maximum adsorption capacity	1265:1291	maximum adsorption capacity	1265:1291	Under optimum conditions, maximum removal efficiency and maximum adsorption capacity were obtained 68% and 142 mg/g, respectively.
31022483	2	33	theme	adsorptive	427:436	arg1	removal					438:444	the adsorptive removal	423:444	the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics	423:507	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	9	34	theme	promisable	1449:1458	arg1	strategy					1460:1467	a promisable strategy	1447:1467	a promisable strategy to remediation of wastewaters	1447:1497	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	0	35	theme	reusability	136:146	arg1	Isotherm					99:106	Isotherm	99:106	Isotherm	99:106	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	0	35	theme	reusability	136:146	arg1	studies					148:154	kinetic, thermodynamic and reusability studies	109:154	kinetic, thermodynamic and reusability studies	109:154	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	3	36	theme	potential	612:620	arg1	meter					622:626	zeta potential meter	607:626	zeta potential meter	607:626	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	8	37	theme	optimum	1214:1220	arg1	conditions					1222:1231	optimum conditions	1214:1231	optimum conditions	1214:1231	Under optimum conditions, maximum removal efficiency and maximum adsorption capacity were obtained 68% and 142 mg/g, respectively.
31022483	8	38	theme	removal	1242:1248	arg1	efficiency					1250:1259	maximum removal efficiency	1234:1259	maximum removal efficiency	1234:1259	Under optimum conditions, maximum removal efficiency and maximum adsorption capacity were obtained 68% and 142 mg/g, respectively.
31022483	8	39	theme	maximum	1234:1240	arg1	efficiency					1250:1259	maximum removal efficiency	1234:1259	maximum removal efficiency	1234:1259	Under optimum conditions, maximum removal efficiency and maximum adsorption capacity were obtained 68% and 142 mg/g, respectively.
31022483	1	40	theme	great	230:234	arg1	deal					236:239	a great deal	228:239	a great deal of attention due to their serious health and environmental problems	228:307	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	0	41	theme	recovery	43:50	arg1	investigation					12:24	Mechanistic investigation	0:24	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan	0:82	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	5	42	theme	RSM	756:758	arg1	models					760:765	the RSM models	752:765	the RSM models	752:765	The reliability of the RSM models was tested by fitting the data.
31022483	8	43	theme	adsorption	1273:1282	arg1	capacity					1284:1291	maximum adsorption capacity	1265:1291	maximum adsorption capacity	1265:1291	Under optimum conditions, maximum removal efficiency and maximum adsorption capacity were obtained 68% and 142 mg/g, respectively.
31022483	7	44	theme	adsorption	969:978	arg1	rate					980:983	low adsorption rate	965:983	low adsorption rate	965:983	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	5	45	theme	models	760:765	arg1	reliability					737:747	The reliability	733:747	The reliability of the RSM models	733:765	The reliability of the RSM models was tested by fitting the data.
31022483	3	46	theme	zeta	607:610	arg1	meter					622:626	zeta potential meter	607:626	zeta potential meter	607:626	The composition and surface morphology of Fe-CS NCs were studied by SEM, BET, XRD, TEM, FTIR and zeta potential meter.
31022483	6	47	theme	results	829:835	arg1	analysis					813:820	A comparative analysis	799:820	A comparative analysis of the results derived from the models	799:859	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	2	48	theme	polymer	355:361	arg1	nanocomposites					363:376	a magnetite imprinted chitosan polymer nanocomposites	324:376	a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs)	324:388	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	2	48	theme	polymer	355:361	arg1	NCs					385:387	Fe-CS NCs	379:387	Fe-CS NCs	379:387	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	0	49	theme	magnetite-imprinted	55:73	arg1	chitosan					75:82	magnetite-imprinted chitosan	55:82	magnetite-imprinted chitosan	55:82	Mechanistic investigation of ciprofloxacin recovery by magnetite-imprinted chitosan nanocomposite: Isotherm, kinetic, thermodynamic and reusability studies.
31022483	6	50	theme	comparative	801:811	arg1	analysis					813:820	A comparative analysis	799:820	A comparative analysis of the results derived from the models	799:859	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	1	51	theme	antibiotics	169:179	arg1	Recovery					157:164	Recovery	157:164	Recovery of antibiotics from water and wastewaters	157:206	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	2	52	theme	chitosan	346:353	arg1	nanocomposites					363:376	a magnetite imprinted chitosan polymer nanocomposites	324:376	a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs)	324:388	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	2	52	theme	chitosan	346:353	arg1	NCs					385:387	Fe-CS NCs	379:387	Fe-CS NCs	379:387	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	9	53	theme	Well	1339:1342	arg1	regenerability					1344:1357	Well regenerability	1339:1357	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal	1339:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	7	54	theme	contour	925:931	arg1	results					942:948	the contour plotting results	921:948	the contour plotting results	921:948	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	2	55	theme	imprinted	336:344	arg1	nanocomposites					363:376	a magnetite imprinted chitosan polymer nanocomposites	324:376	a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs)	324:388	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	2	55	theme	imprinted	336:344	arg1	NCs					385:387	Fe-CS NCs	379:387	Fe-CS NCs	379:387	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	7	56	theme	low	965:967	arg1	rate					980:983	low adsorption rate	965:983	low adsorption rate	965:983	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	4	57	theme	surface	706:712	arg1	RSM					727:729	RSM	727:729	RSM	727:729	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	4	57	theme	surface	706:712	arg1	methodology					714:724	response surface methodology	697:724	response surface methodology (RSM)	697:730	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	2	58	theme	magnetite	326:334	arg1	nanocomposites					363:376	a magnetite imprinted chitosan polymer nanocomposites	324:376	a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs)	324:388	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	2	58	theme	magnetite	326:334	arg1	NCs					385:387	Fe-CS NCs	379:387	Fe-CS NCs	379:387	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	1	59	theme	attention	244:252	arg1	deal					236:239	a great deal	228:239	a great deal of attention due to their serious health and environmental problems	228:307	Recovery of antibiotics from water and wastewaters has recently gained a great deal of attention due to their serious health and environmental problems.
31022483	4	60	theme	adsorption	659:668	arg1	process					670:676	adsorption process	659:676	adsorption process	659:676	Modelling and optimization of adsorption process were studied using response surface methodology (RSM).
31022483	9	61	theme	Fe-CS	1362:1366	arg1	regenerability					1344:1357	Well regenerability	1339:1357	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal	1339:1437	Well regenerability of Fe-CS together with its high capacity of fluoroquinolone antibiotics removal provide a promisable strategy to remediation of wastewaters.
31022483	7	62	theme	electrostatic	1151:1163	arg1	interactions					1165:1176	the electrostatic interactions	1147:1176	the electrostatic interactions	1147:1176	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	6	63	theme	second-order	883:894	arg1	model					896:900	the second-order model	879:900	the second-order model	879:900	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	6	63	theme	second-order	883:894	arg1	best					910:913	best	910:913	best	910:913	A comparative analysis of the results derived from the models demonstrated that the second-order model was the best.
31022483	7	64	theme	plotting	933:940	arg1	results					942:948	the contour plotting results	921:948	the contour plotting results	921:948	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
31022483	2	65	theme	Fe-CS	379:383	arg1	nanocomposites					363:376	a magnetite imprinted chitosan polymer nanocomposites	324:376	a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs)	324:388	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	2	65	theme	Fe-CS	379:383	arg1	NCs					385:387	Fe-CS NCs	379:387	Fe-CS NCs	379:387	In this work, a magnetite imprinted chitosan polymer nanocomposites (Fe-CS NCs) were synthesized and applied for the adsorptive removal of ciprofloxacin (CIFO) as a model fluoroquinolone antibiotics.
31022483	7	66	dep	observed	989:996	arg1	whereas					1045:1051	whereas	1045:1051	whereas	1045:1051	From the contour plotting results, at pH < pHiep low adsorption rate was observed due to protonation of the chitosan NH2 groups, whereas the adsorption rate was significantly enhanced and achieved to a maximum level at pH 6 due to the electrostatic interactions and hydrophobic interactions.
30274055	4	0	theme	membranes	903:911	arg1	cross-sections					885:898	cross-sections	885:898	cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components	885:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	5	1	dep	19 kPa	1320:1325	arg1	10 kPa					1310:1315	10 kPa	1310:1315	10 kPa	1310:1315	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	7	2	from	relevance	1772:1780	arg1	GTR					1785:1787	GTR	1785:1787	GTR	1785:1787	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	7	3	dep	%	1695:1695	arg1	%					1687:1687	%	1687:1687	%	1687:1687	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	0	4	with	membranes	95:103	arg1	interface					130:138	rapidly mineralized interface	110:138	rapidly mineralized interface in relevance to guided tissue regeneration	110:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	3	5	theme	GEN	569:571	arg1	products					587:594	GEN cross-linking products	569:594	GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters)	569:647	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	2	6	theme	/gelatin	393:400	arg1	membranes					416:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	3	7	theme	secondary	606:614	arg1	amines					626:631	the secondary CHT (GEL) amines	602:631	the secondary CHT (GEL) amines	602:631	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	1	8	theme	advanced	223:230	arg1	materials					232:240	the advanced materials	219:240	the advanced materials in guided tissue regeneration (GTR) concept	219:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	5	9	from	compression	1292:1302	arg1	stress					1282:1287	the yield stress	1272:1287	the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa)	1272:1370	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	3	10	theme	physical	741:748	arg1	integration					750:760	physical integration	741:760	the chemical inter-linking as well as physical integration between interface layers	703:785	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	7	11	from	swelling	1664:1671	arg1	4 weeks					1712:1718	4 weeks	1712:1718	4 weeks in vitro degradation experiment	1712:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	0	12	theme	tissue	163:168	arg1	regeneration					170:181	guided tissue regeneration	156:181	guided tissue regeneration	156:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	4	13	from	contribution	944:955	arg1	contrast					960:967	contrast	960:967	contrast	960:967	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	0	14	theme	integration	37:47	arg1	assessment					23:32	Autofluorescence-aided assessment	0:32	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.	0:182	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	1	15	theme	tissue	252:257	arg1	concept					278:284	guided tissue regeneration (GTR) concept	245:284	guided tissue regeneration (GTR) concept	245:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	1	16	from	materials	232:240	arg1	concept					278:284	guided tissue regeneration (GTR) concept	245:284	guided tissue regeneration (GTR) concept	245:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	4	17	theme	prevalent	930:938	arg1	contribution					944:955	(by weight) prevalent GEL contribution	918:955	(by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components	918:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	3	18	dep	products	587:594	arg1	amines					626:631	the secondary CHT (GEL) amines	602:631	the secondary CHT (GEL) amines	602:631	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	3	18	dep	products	587:594	arg1	esters					641:646	GEN esters	637:646	GEN esters	637:646	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	4	19	with	membranes	903:911	arg1	contribution					944:955	(by weight) prevalent GEL contribution	918:955	(by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components	918:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	7	20	theme	high	1623:1626	arg1	swelling					1664:1671	The fast and high (up to 580%), composition-dependent swelling	1610:1671	The fast and high (up to 580%), composition-dependent swelling	1610:1671	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	6	21	theme	rapid	1377:1381	arg1	procedure					1398:1406	The rapid mineralization procedure	1373:1406	The rapid mineralization procedure	1373:1406	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	6	22	theme	osseointegrative	1582:1597	arg1	interface					1599:1607	osseointegrative interface	1582:1607	osseointegrative interface	1582:1607	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	7	23	theme	weight	1697:1702	arg1	loss					1704:1707	67% to 100% weight loss	1685:1707	67% to 100% weight loss in 4 weeks in vitro degradation experiment	1685:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	7	24	theme	fast	1614:1617	arg1	swelling					1664:1671	The fast and high (up to 580%), composition-dependent swelling	1610:1671	The fast and high (up to 580%), composition-dependent swelling	1610:1671	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	4	25	theme	equal	1017:1021	arg1	presence					1023:1030	equal presence	1017:1030	equal presence of components	1017:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	5	26	theme	yield	1276:1280	arg1	stress					1282:1287	the yield stress	1272:1287	the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa)	1272:1370	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	1	27	theme	barrier	201:207	arg1	function					209:216	barrier function	201:216	barrier function	201:216	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	7	28	theme	100	1692:1694	arg1	%					1687:1687	%	1687:1687	%	1687:1687	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	3	29	from	products	587:594	arg1	signal					557:562	the autofluorescence signal	536:562	the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters)	536:647	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	0	30	theme	mineralized	118:128	arg1	interface					130:138	rapidly mineralized interface	110:138	rapidly mineralized interface in relevance to guided tissue regeneration	110:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	5	31	theme	pore	1140:1143	arg1	distributions					1150:1162	the pore size distributions	1136:1162	the pore size distributions	1136:1162	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	2	32	theme	bilayer	408:414	arg1	membranes					416:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	4	33	dep	prevalent	930:938	arg1	weight					922:927	by weight	919:927	by weight	919:927	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	2	34	theme	GEL	403:405	arg1	membranes					416:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	0	35	theme	Autofluorescence-aided	0:21	arg1	assessment					23:32	Autofluorescence-aided assessment	0:32	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.	0:182	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	5	36	dep	34 kPa	1364:1369	arg1	26 kPa					1354:1359	26 kPa	1354:1359	26 kPa	1354:1359	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	2	37	theme	freeze-casting	454:467	arg1	procedures					469:478	successive solvent- and freeze-casting procedures	430:478	successive solvent- and freeze-casting procedures	430:478	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	7	38	dep	loss	1704:1707	arg1	%					1695:1695	%	1695:1695	%	1695:1695	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	2	39	theme	solvent-	441:448	arg1	procedures					469:478	successive solvent- and freeze-casting procedures	430:478	successive solvent- and freeze-casting procedures	430:478	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	3	40	theme	Confocal	654:661	arg1	Microscopy					675:684	the Confocal Fluorescent Microscopy	650:684	the Confocal Fluorescent Microscopy (CFM)	650:690	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	3	40	theme	Confocal	654:661	arg1	CFM					687:689	CFM	687:689	CFM	687:689	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	1	41	theme	distinct	331:338	arg1	tissues					352:358	distinct interfacing tissues	331:358	distinct interfacing tissues	331:358	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	5	42	theme	constant	1051:1058	arg1	conditions					1071:1080	The constant processing conditions	1047:1080	The constant processing conditions on variable compositions	1047:1105	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	0	43	theme	bilayer	87:93	arg1	membranes					95:103	chitosan/gelatin bilayer membranes	70:103	chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration	70:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	4	44	theme	regions	854:860	arg1	presence					792:799	The presence	788:799	The presence of non- and highly μ-porous and pore-interconnecting regions	788:860	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	2	45	theme	chitosan	379:386	arg1	membranes					416:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	chitosan (CHT)/gelatin (GEL) bilayer membranes	379:424	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	5	46	theme	pore	1191:1194	arg1	thickness					1201:1209	pore wall thickness	1191:1209	pore wall thickness	1191:1209	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	4	47	with	membrane	1003:1010	arg1	presence					1023:1030	equal presence	1017:1030	equal presence of components	1017:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	4	48	theme	non-	804:807	arg1	regions					854:860	non- and highly μ-porous and pore-interconnecting regions	804:860	non- and highly μ-porous and pore-interconnecting regions	804:860	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	1	49	theme	regeneration	259:270	arg1	concept					278:284	guided tissue regeneration (GTR) concept	245:284	guided tissue regeneration (GTR) concept	245:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	3	50	theme	autofluorescence	540:555	arg1	signal					557:562	the autofluorescence signal	536:562	the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters)	536:647	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	6	51	theme	carbonated	1498:1507	arg1	apatite					1509:1515	nonstoichiometric carbonated apatite	1480:1515	nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range	1480:1547	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	1	52	theme	GTR	273:275	arg1	concept					278:284	guided tissue regeneration (GTR) concept	245:284	guided tissue regeneration (GTR) concept	245:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	3	53	theme	chemical	707:714	arg1	inter-linking					716:728	the chemical inter-linking	703:728	the chemical inter-linking as well as physical integration between interface layers	703:785	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	6	54	with	apatite	1509:1515	arg1	ration					1527:1532	Ca/P ration	1522:1532	Ca/P ration	1522:1532	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	4	55	from	organization	987:998	arg1	membrane					1003:1010	membrane	1003:1010	membrane with equal presence of components	1003:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	3	56	theme	cross-linking	573:585	arg1	products					587:594	GEN cross-linking products	569:594	GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters)	569:647	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	6	57	theme	minerals	1459:1466	arg1	deposition					1420:1429	deposition	1420:1429	deposition	1420:1429	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	5	58	theme	GEL	1238:1240	arg1	increase					1242:1249	GEL increase	1238:1249	GEL increase	1238:1249	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	2	59	theme	genipin	484:490	arg1	chemistry					512:520	genipin (GEN) cross-linking chemistry	484:520	genipin (GEN) cross-linking chemistry	484:520	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	7	60	from	loss	1704:1707	arg1	4 weeks					1712:1718	4 weeks	1712:1718	4 weeks in vitro degradation experiment	1712:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	1	61	theme	tissues	352:358	arg1	regeneration					315:326	regeneration	315:326	regeneration of distinct interfacing tissues	315:358	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	7	62	theme	degradation	1729:1739	arg1	experiment					1741:1750	vitro degradation experiment	1723:1750	vitro degradation experiment	1723:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	7	63	from	4 weeks	1712:1718	arg1	experiment					1741:1750	vitro degradation experiment	1723:1750	vitro degradation experiment	1723:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	3	64	theme	interface	770:778	arg1	layers					780:785	interface layers	770:785	interface layers	770:785	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	0	65	theme	guided	156:161	arg1	regeneration					170:181	guided tissue regeneration	156:181	guided tissue regeneration	156:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	6	66	theme	1.7-2	1537:1541	arg1	range					1543:1547	1.7-2 range	1537:1547	1.7-2 range	1537:1547	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	3	67	dep	amines	626:631	arg1	i.e.					597:600	i.e.	597:600	i.e.	597:600	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	4	68	theme	GEL	940:942	arg1	contribution					944:955	(by weight) prevalent GEL contribution	918:955	(by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components	918:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	7	69	dep	fast	1614:1617	arg1	%					1638:1638	up to 580%	1629:1638	up to 580%	1629:1638	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	7	69	dep	fast	1614:1617	arg1	composition-dependent					1642:1662	composition-dependent	1642:1662	composition-dependent	1642:1662	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	3	70	theme	GEL	621:623	arg1	amines					626:631	the secondary CHT (GEL) amines	602:631	the secondary CHT (GEL) amines	602:631	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	1	71	theme	guided	245:250	arg1	concept					278:284	guided tissue regeneration (GTR) concept	245:284	guided tissue regeneration (GTR) concept	245:284	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	3	72	theme	CHT	616:618	arg1	amines					626:631	the secondary CHT (GEL) amines	602:631	the secondary CHT (GEL) amines	602:631	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	4	73	theme	by	919:920	arg1	weight					922:927	by weight	919:927	by weight	919:927	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	7	74	dep	%	1687:1687	arg1	to					1689:1690	to	1689:1690	to	1689:1690	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	5	75	theme	variable	1085:1092	arg1	compositions					1094:1105	variable compositions	1085:1105	variable compositions	1085:1105	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	3	76	theme	GEN	637:639	arg1	esters					641:646	GEN esters	637:646	GEN esters	637:646	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	7	77	theme	vitro	1723:1727	arg1	experiment					1741:1750	vitro degradation experiment	1723:1750	vitro degradation experiment	1723:1750	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	0	78	from	interface	130:138	arg1	relevance					143:151	relevance	143:151	relevance	143:151	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	5	79	from	conditions	1071:1080	arg1	compositions					1094:1105	variable compositions	1085:1105	variable compositions	1085:1105	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	6	80	theme	mineralization	1383:1396	arg1	procedure					1398:1406	The rapid mineralization procedure	1373:1406	The rapid mineralization procedure	1373:1406	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	6	81	theme	interface	1599:1607	arg1	formation					1569:1577	formation	1569:1577	formation of osseointegrative interface	1569:1607	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	5	82	from	modulus	1340:1346	arg1	stress					1282:1287	the yield stress	1272:1287	the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa)	1272:1370	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	5	83	theme	1-230 μm	1168:1175	arg1	range					1177:1181	1-230 μm range	1168:1181	1-230 μm range	1168:1181	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	2	84	theme	cross-linking	498:510	arg1	chemistry					512:520	genipin (GEN) cross-linking chemistry	484:520	genipin (GEN) cross-linking chemistry	484:520	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	0	85	from	assessment	23:32	arg1	membranes					95:103	chitosan/gelatin bilayer membranes	70:103	chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration	70:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	5	86	theme	elastic	1332:1338	arg1	modulus					1340:1346	elastic modulus	1332:1346	elastic modulus (from 26 kPa to 34 kPa)	1332:1370	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	5	87	theme	size	1145:1148	arg1	distributions					1150:1162	the pore size distributions	1136:1162	the pore size distributions	1136:1162	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	4	88	theme	sheet-like	976:985	arg1	organization					987:998	the sheet-like organization	972:998	the sheet-like organization in membrane with equal presence of components	972:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	6	89	theme	spherical	1449:1457	arg1	minerals					1459:1466	non-regular to spherical minerals	1434:1466	non-regular to spherical minerals	1434:1466	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	7	90	dep	580	1635:1637	arg1	to					1632:1633	to	1632:1633	to	1632:1633	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	7	91	theme	67	1685:1686	arg1	%					1687:1687	%	1687:1687	%	1687:1687	The fast and high (up to 580%), composition-dependent swelling, as well as 67% to 100% weight loss in 4 weeks in vitro degradation experiment point on membranes' relevance in GTR.
30274055	6	92	theme	non-regular	1434:1444	arg1	minerals					1459:1466	non-regular to spherical minerals	1434:1466	non-regular to spherical minerals	1434:1466	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	2	93	theme	successive	430:439	arg1	procedures					469:478	successive solvent- and freeze-casting procedures	430:478	successive solvent- and freeze-casting procedures	430:478	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	0	94	theme	μ-structuring	53:65	arg1	assessment					23:32	Autofluorescence-aided assessment	0:32	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.	0:182	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	6	95	from	apatite	1509:1515	arg1	range					1543:1547	1.7-2 range	1537:1547	1.7-2 range	1537:1547	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	2	96	theme	GEN	493:495	arg1	chemistry					512:520	genipin (GEN) cross-linking chemistry	484:520	genipin (GEN) cross-linking chemistry	484:520	Herein we develop chitosan (CHT)/gelatin (GEL) bilayer membranes via successive solvent- and freeze-casting procedures and genipin (GEN) cross-linking chemistry.
30274055	4	97	theme	μ-porous	820:827	arg1	regions					854:860	non- and highly μ-porous and pore-interconnecting regions	804:860	non- and highly μ-porous and pore-interconnecting regions	804:860	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	0	98	theme	chitosan/gelatin	70:85	arg1	membranes					95:103	chitosan/gelatin bilayer membranes	70:103	chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration	70:181	Autofluorescence-aided assessment of integration and μ-structuring in chitosan/gelatin bilayer membranes with rapidly mineralized interface in relevance to guided tissue regeneration.
30274055	1	99	theme	interfacing	340:350	arg1	tissues					352:358	distinct interfacing tissues	331:358	distinct interfacing tissues	331:358	Beyond providing barrier function, the advanced materials in guided tissue regeneration (GTR) concept are further prompt to foster regeneration of distinct interfacing tissues.
30274055	5	100	theme	processing	1060:1069	arg1	conditions					1071:1080	The constant processing conditions	1047:1080	The constant processing conditions on variable compositions	1047:1105	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	6	101	dep	minerals	1459:1466	arg1	to					1446:1447	to	1446:1447	to	1446:1447	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	4	102	theme	components	1035:1044	arg1	presence					1023:1030	equal presence	1017:1030	equal presence of components	1017:1044	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	4	103	theme	pore-interconnecting	833:852	arg1	regions					854:860	non- and highly μ-porous and pore-interconnecting regions	804:860	non- and highly μ-porous and pore-interconnecting regions	804:860	The presence of non- and highly μ-porous and pore-interconnecting regions is demonstrated within cross-sections of membranes with (by weight) prevalent GEL contribution in contrast to the sheet-like organization in membrane with equal presence of components.
30274055	6	104	theme	Ca/P	1522:1525	arg1	ration					1527:1532	Ca/P ration	1522:1532	Ca/P ration	1522:1532	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	5	105	theme	wall	1196:1199	arg1	thickness					1201:1209	pore wall thickness	1191:1209	pore wall thickness	1191:1209	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	6	106	contain	containing	1469:1478	arg2	apatite					1509:1515	nonstoichiometric carbonated apatite	1480:1515	nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range	1480:1547	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	6	106	contain	containing	1469:1478	arg1	deposition					1420:1429	deposition	1420:1429	deposition	1420:1429	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
30274055	5	107	dep	affect	1129:1134	arg1	range					1177:1181	1-230 μm range	1168:1181	1-230 μm range	1168:1181	The constant processing conditions on variable compositions did not significantly affect the pore size distributions (in 1-230 μm range), while pore wall thickness increase up to 220 μm with GEL increase, which also improves the yield stress at compression (from 10 kPa to 19 kPa) and elastic modulus (from 26 kPa to 34 kPa).
30274055	3	108	theme	Fluorescent	663:673	arg1	Microscopy					675:684	the Confocal Fluorescent Microscopy	650:684	the Confocal Fluorescent Microscopy (CFM)	650:690	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	3	108	theme	Fluorescent	663:673	arg1	CFM					687:689	CFM	687:689	CFM	687:689	By utilizing the autofluorescence signal from GEN cross-linking products (i.e. the secondary CHT (GEL) amines and GEN esters), the Confocal Fluorescent Microscopy (CFM) identifies the chemical inter-linking as well as physical integration between interface layers.
30274055	6	109	theme	nonstoichiometric	1480:1496	arg1	apatite					1509:1515	nonstoichiometric carbonated apatite	1480:1515	nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range	1480:1547	The rapid mineralization procedure resulted in deposition of non-regular to spherical minerals, containing nonstoichiometric carbonated apatite with Ca/P ration in 1.7-2 range, which demonstrates formation of osseointegrative interface.
31614468	10	0	theme	of	1647:1648	arg1	wounds					1650:1655	the clinical healing of wounds	1626:1655	the clinical healing of wounds	1626:1655	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	9	1	contain	had	1500:1502	arg1	dressings					1490:1498	the dressings	1486:1498	the dressings	1486:1498	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	9	1	contain	had	1500:1502	arg2	biocompatibility					1519:1534	excellent cell biocompatibility	1504:1534	excellent cell biocompatibility	1504:1534	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	9	2	theme	cell	1514:1517	arg1	biocompatibility					1519:1534	excellent cell biocompatibility	1504:1534	excellent cell biocompatibility	1504:1534	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	2	3	theme	unique	328:333	arg1	performance					347:357	unique gel forming performance	328:357	unique gel forming performance	328:357	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	8	4	theme	burst	1347:1351	arg1	release					1353:1359	the burst release	1343:1359	the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time	1343:1452	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	4	5	theme	treated	664:670	arg1	nonwovens					691:699	plasma treated CAF needle-punched nonwovens	657:699	plasma treated CAF needle-punched nonwovens	657:699	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	2	6	theme	forming	339:345	arg1	performance					347:357	unique gel forming performance	328:357	unique gel forming performance	328:357	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	5	7	theme	porous	915:920	arg1	structure					932:940	a porous composite structure	913:940	a porous composite structure	913:940	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	8	8	theme	CM-Chit	1321:1327	arg1	gel					1329:1331	CM-Chit gel	1321:1331	CM-Chit gel	1321:1331	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	6	9	theme	Skin-like	1024:1032	arg1	performance					1042:1052	Skin-like tensile performance	1024:1052	Skin-like tensile performance	1024:1052	Skin-like tensile performance allowed the dressings to provide a better healing environment.
31614468	4	10	theme	needle-punched	676:689	arg1	nonwovens					691:699	plasma treated CAF needle-punched nonwovens	657:699	plasma treated CAF needle-punched nonwovens	657:699	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	3	11	with	structure	508:516	arg1	properties					539:548	weak mechanical properties	523:548	weak mechanical properties	523:548	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	4	12	theme	composite	749:757	arg1	dressings					759:767	Mino loaded CM-Chit gel/CAF nonwovens composite dressings	711:767	Mino loaded CM-Chit gel/CAF nonwovens composite dressings	711:767	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	2	13	theme	alginate	264:271	arg1	dressings					285:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	10	14	contain	had	1599:1601	arg2	dressings					1555:1563	new functional dressings	1540:1563	new functional dressings	1540:1563	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	10	14	contain	had	1599:1601	arg1	dressings					1589:1597	the prepared composite dressings	1566:1597	the prepared composite dressings	1566:1597	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	10	14	contain	had	1599:1601	arg2	potential					1613:1621	excellent potential	1603:1621	excellent potential	1603:1621	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	7	15	theme	Antibacterial	1117:1129	arg1	assay					1131:1135	Antibacterial assay	1117:1135	Antibacterial assay against Escherichia coli and Staphylococcus aureus	1117:1186	Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings.
31614468	6	16	theme	healing	1096:1102	arg1	environment					1104:1114	a better healing environment	1087:1114	a better healing environment	1087:1114	Skin-like tensile performance allowed the dressings to provide a better healing environment.
31614468	4	17	theme	loaded	716:721	arg1	nonwovens					739:747	loaded CM-Chit gel/CAF nonwovens	716:747	loaded CM-Chit gel/CAF nonwovens	716:747	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	0	18	theme	Nonwovens	85:93	arg1	Dressings					111:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	4	19	theme	gel/CAF	731:737	arg1	nonwovens					739:747	loaded CM-Chit gel/CAF nonwovens	716:747	loaded CM-Chit gel/CAF nonwovens	716:747	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	0	20	theme	Wound	105:109	arg1	Dressings					111:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	10	21	theme	new	1540:1542	arg1	potential					1613:1621	excellent potential	1603:1621	excellent potential	1603:1621	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	10	21	theme	new	1540:1542	arg1	dressings					1555:1563	new functional dressings	1540:1563	new functional dressings	1540:1563	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	8	22	theme	release	1441:1447	arg1	time					1449:1452	release time	1441:1452	release time	1441:1452	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	10	23	theme	composite	1579:1587	arg1	dressings					1589:1597	the prepared composite dressings	1566:1597	the prepared composite dressings	1566:1597	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	0	24	theme	Carboxymethyl	49:61	arg1	Chitosan					63:70	Minocycline-Loaded Carboxymethyl Chitosan	30:70	Minocycline-Loaded Carboxymethyl Chitosan	30:70	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	1	25	from	biomaterials	157:168	arg1	derivatives					125:135	derivatives	125:135	derivatives from marine natural biomaterials	125:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	1	25	from	biomaterials	157:168	arg1	biomaterials					205:216	alginate-based and chitosan-based biomaterials	171:216	alginate-based and chitosan-based biomaterials	171:216	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	4	26	dep	Mino	711:714	arg1	nonwovens					739:747	loaded CM-Chit gel/CAF nonwovens	716:747	loaded CM-Chit gel/CAF nonwovens	716:747	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	3	27	theme	Carboxymethyl	425:437	arg1	CM-Chit					449:455	CM-Chit	449:455	CM-Chit	449:455	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	3	27	theme	Carboxymethyl	425:437	arg1	chitosan					439:446	Carboxymethyl chitosan	425:446	Carboxymethyl chitosan (CM-Chit)	425:456	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	3	28	theme	excellent	462:470	arg1	activity					486:493	excellent antibacterial activity	462:493	excellent antibacterial activity	462:493	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	7	29	theme	dressings	1287:1295	arg1	activity					1265:1272	the antibacterial activity	1247:1272	the antibacterial activity of the wound dressings	1247:1295	Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings.
31614468	5	30	theme	wound	1008:1012	arg1	exudates					1014:1021	wound exudates	1008:1021	wound exudates	1008:1021	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	4	31	theme	minocycline	592:602	arg1	solution					619:626	minocycline (Mino)/CM-Chit solution	592:626	minocycline (Mino)/CM-Chit solution	592:626	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	3	32	theme	gel	504:506	arg1	structure					508:516	the gel structure	500:516	the gel structure with weak mechanical properties	500:548	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	4	33	theme	Mino	605:608	arg1	solution					619:626	minocycline (Mino)/CM-Chit solution	592:626	minocycline (Mino)/CM-Chit solution	592:626	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	0	34	theme	Minocycline-Loaded	30:47	arg1	Chitosan					63:70	Minocycline-Loaded Carboxymethyl Chitosan	30:70	Minocycline-Loaded Carboxymethyl Chitosan	30:70	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	9	35	theme	Cell	1455:1458	arg1	assay					1468:1472	Cell culture assay	1455:1472	Cell culture assay	1455:1472	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	10	36	theme	healing	1639:1645	arg1	wounds					1650:1655	the clinical healing of wounds	1626:1655	the clinical healing of wounds	1626:1655	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	3	37	theme	mechanical	528:537	arg1	properties					539:548	weak mechanical properties	523:548	weak mechanical properties	523:548	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	0	38	theme	Chitosan	63:70	arg1	Properties					16:25	Properties	16:25	Properties	16:25	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	0	38	theme	Chitosan	63:70	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	2	39	theme	low	368:370	arg1	bioactivity					372:382	the low bioactivity	364:382	the low bioactivity	364:382	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	2	40	theme	wound	410:414	arg1	healing					416:422	wound healing	410:422	wound healing	410:422	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	7	41	theme	Mino	1219:1222	arg1	addition					1207:1214	the addition	1203:1214	the addition of Mino	1203:1222	Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings.
31614468	9	42	theme	excellent	1504:1512	arg1	biocompatibility					1519:1534	excellent cell biocompatibility	1504:1534	excellent cell biocompatibility	1504:1534	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	4	43	theme	/CM-Chit	610:617	arg1	solution					619:626	minocycline (Mino)/CM-Chit solution	592:626	minocycline (Mino)/CM-Chit solution	592:626	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	2	44	theme	gel	335:337	arg1	performance					347:357	unique gel forming performance	328:357	unique gel forming performance	328:357	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	2	45	contain	possess	295:301	arg1	dressings					285:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	2	45	contain	possess	295:301	arg2	performance					347:357	unique gel forming performance	328:357	unique gel forming performance	328:357	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	2	45	contain	possess	295:301	arg2	absorption					313:322	excellent absorption	303:322	excellent absorption	303:322	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	3	46	contain	has	458:460	arg1	CM-Chit					449:455	CM-Chit	449:455	CM-Chit	449:455	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	3	46	contain	has	458:460	arg1	chitosan					439:446	Carboxymethyl chitosan	425:446	Carboxymethyl chitosan (CM-Chit)	425:456	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	3	46	contain	has	458:460	arg2	activity					486:493	excellent antibacterial activity	462:493	excellent antibacterial activity	462:493	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	2	47	from	application	395:405	arg1	healing					416:422	wound healing	410:422	wound healing	410:422	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	4	48	theme	nonwovens	691:699	arg1	surface					646:652	the surface	642:652	the surface of plasma treated CAF needle-punched nonwovens	642:699	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	1	49	theme	wound	239:243	arg1	dressings					245:253	wound dressings	239:253	wound dressings	239:253	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	8	50	theme	tight	1302:1306	arg1	structure					1308:1316	The tight structure	1298:1316	The tight structure of CM-Chit gel	1298:1331	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	6	51	theme	tensile	1034:1040	arg1	performance					1042:1052	Skin-like tensile performance	1024:1052	Skin-like tensile performance	1024:1052	Skin-like tensile performance allowed the dressings to provide a better healing environment.
31614468	5	52	contain	had	909:911	arg2	structure					932:940	a porous composite structure	913:940	a porous composite structure	913:940	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	5	52	contain	had	909:911	arg1	dressings					899:907	The dressings	895:907	The dressings	895:907	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	4	53	theme	CM-Chit	723:729	arg1	nonwovens					739:747	loaded CM-Chit gel/CAF nonwovens	716:747	loaded CM-Chit gel/CAF nonwovens	716:747	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	4	54	theme	plasma	657:662	arg1	nonwovens					691:699	plasma treated CAF needle-punched nonwovens	657:699	plasma treated CAF needle-punched nonwovens	657:699	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	2	55	theme	CAF	280:282	arg1	dressings					285:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	5	56	theme	composite	922:930	arg1	structure					932:940	a porous composite structure	913:940	a porous composite structure	913:940	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	4	57	theme	CAF	672:674	arg1	nonwovens					691:699	plasma treated CAF needle-punched nonwovens	657:699	plasma treated CAF needle-punched nonwovens	657:699	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	2	58	theme	fiber	273:277	arg1	dressings					285:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	1	59	theme	marine	142:147	arg1	biomaterials					157:168	marine natural biomaterials	142:168	marine natural biomaterials	142:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	8	60	theme	gel	1329:1331	arg1	structure					1308:1316	The tight structure	1298:1316	The tight structure of CM-Chit gel	1298:1331	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	2	61	theme	excellent	303:311	arg1	absorption					313:322	excellent absorption	303:322	excellent absorption	303:322	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	5	62	theme	large	992:996	arg1	number					998:1003	a large number	990:1003	a large number of wound exudates	990:1021	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	8	63	contain	had	1391:1393	arg1	dressings					1381:1389	the dressings	1377:1389	the dressings	1377:1389	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	8	63	contain	had	1391:1393	arg2	activity					1409:1416	antibacterial activity	1395:1416	antibacterial activity	1395:1416	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	1	64	used	used	231:234	arg2	biomaterials					205:216	alginate-based and chitosan-based biomaterials	171:216	alginate-based and chitosan-based biomaterials	171:216	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	1	64	used	used	231:234	arg2	derivatives					125:135	derivatives	125:135	derivatives from marine natural biomaterials	125:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	8	65	theme	antibacterial	1395:1407	arg1	activity					1409:1416	antibacterial activity	1395:1416	antibacterial activity	1395:1416	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	0	66	theme	Composite	95:103	arg1	Dressings					111:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	1	67	theme	alginate-based	171:184	arg1	derivatives					125:135	derivatives	125:135	derivatives from marine natural biomaterials	125:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	1	67	theme	alginate-based	171:184	arg1	biomaterials					205:216	alginate-based and chitosan-based biomaterials	171:216	alginate-based and chitosan-based biomaterials	171:216	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	1	68	theme	natural	149:155	arg1	biomaterials					157:168	marine natural biomaterials	142:168	marine natural biomaterials	142:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	6	69	theme	better	1089:1094	arg1	environment					1104:1114	a better healing environment	1087:1114	a better healing environment	1087:1114	Skin-like tensile performance allowed the dressings to provide a better healing environment.
31614468	4	70	theme	Mino	711:714	arg1	dressings					759:767	Mino loaded CM-Chit gel/CAF nonwovens composite dressings	711:767	Mino loaded CM-Chit gel/CAF nonwovens composite dressings	711:767	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	1	71	theme	chitosan-based	190:203	arg1	derivatives					125:135	derivatives	125:135	derivatives from marine natural biomaterials	125:168	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	1	71	theme	chitosan-based	190:203	arg1	biomaterials					205:216	alginate-based and chitosan-based biomaterials	171:216	alginate-based and chitosan-based biomaterials	171:216	As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings.
31614468	10	72	theme	functional	1544:1553	arg1	potential					1613:1621	excellent potential	1603:1621	excellent potential	1603:1621	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	10	72	theme	functional	1544:1553	arg1	dressings					1555:1563	new functional dressings	1540:1563	new functional dressings	1540:1563	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	8	73	theme	certain	1423:1429	arg1	period					1431:1436	a certain period	1421:1436	a certain period of release time	1421:1452	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	8	74	theme	Mino	1364:1367	arg1	release					1353:1359	the burst release	1343:1359	the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time	1343:1452	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	10	75	theme	prepared	1570:1577	arg1	dressings					1589:1597	the prepared composite dressings	1566:1597	the prepared composite dressings	1566:1597	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	8	76	theme	time	1449:1452	arg1	period					1431:1436	a certain period	1421:1436	a certain period of release time	1421:1452	The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time.
31614468	7	77	theme	wound	1281:1285	arg1	dressings					1287:1295	the wound dressings	1277:1295	the wound dressings	1277:1295	Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings.
31614468	0	78	dep	Dressings	111:119	arg1	Properties					16:25	Properties	16:25	Properties	16:25	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	0	78	dep	Dressings	111:119	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	3	79	theme	antibacterial	472:484	arg1	activity					486:493	excellent antibacterial activity	462:493	excellent antibacterial activity	462:493	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
31614468	5	80	theme	exudates	1014:1021	arg1	number					998:1003	a large number	990:1003	a large number of wound exudates	990:1021	The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates.
31614468	10	81	theme	excellent	1603:1611	arg1	dressings					1555:1563	new functional dressings	1540:1563	new functional dressings	1540:1563	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	10	81	theme	excellent	1603:1611	arg1	potential					1613:1621	excellent potential	1603:1621	excellent potential	1603:1621	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	4	82	theme	EDC/NHS	788:794	arg1	crosslinking					881:892	EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking	788:892	EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking	788:892	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	7	83	theme	antibacterial	1251:1263	arg1	activity					1265:1272	the antibacterial activity	1247:1272	the antibacterial activity of the wound dressings	1247:1295	Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings.
31614468	0	84	theme	Gel/Alginate	72:83	arg1	Dressings					111:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Gel/Alginate Nonwovens Composite Wound Dressings	72:119	Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.
31614468	9	85	theme	culture	1460:1466	arg1	assay					1468:1472	Cell culture assay	1455:1472	Cell culture assay	1455:1472	Cell culture assay showed that the dressings had excellent cell biocompatibility.
31614468	10	86	theme	clinical	1630:1637	arg1	wounds					1650:1655	the clinical healing of wounds	1626:1655	the clinical healing of wounds	1626:1655	As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.
31614468	2	87	theme	Calcium	256:262	arg1	dressings					285:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings	256:293	Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing.
31614468	4	88	theme	-3-ethylcarbodiimide	824:843	arg1	EDC/NHS					788:794	EDC/NHS	788:794	EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking	788:892	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	4	88	theme	-3-ethylcarbodiimide	824:843	arg1	hydrochloride/N-hydroxysuccinimide					845:878	1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide	797:878	1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide	797:878	In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking.
31614468	3	89	theme	weak	523:526	arg1	properties					539:548	weak mechanical properties	523:548	weak mechanical properties	523:548	Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application.
30890341	11	0	theme	bio-composites	1515:1528	arg1	exploration					1473:1483	exploration	1473:1483	exploration	1473:1483	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	11	0	theme	bio-composites	1515:1528	arg1	fabrication					1457:1467	fabrication	1457:1467	fabrication	1457:1467	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	6	1	with	particles	774:782	arg1	ductility					794:802	good ductility	789:802	good ductility	789:802	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	11	2	theme	high-performance	1488:1503	arg1	bio-composites					1515:1528	high-performance PLA-based bio-composites	1488:1528	high-performance PLA-based bio-composites	1488:1528	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	4	3	theme	PLA	556:558	arg1	strength					532:539	strength	532:539	strength	532:539	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	3	theme	PLA	556:558	arg1	modulus					545:551	modulus	545:551	modulus	545:551	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	0	4	theme	d-lactic	55:62	arg1	oligo					49:53	oligo	49:53	oligo	49:53	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	0	4	theme	d-lactic	55:62	arg1	acid					64:67	d-lactic acid	55:67	d-lactic acid	55:67	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	7	5	theme	PLLA	917:920	arg1	matrix					922:927	PLLA matrix	917:927	PLLA matrix	917:927	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	4	6	contain	have	487:490	arg2	limitations					498:508	their limitations	492:508	their limitations	492:508	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	6	contain	have	487:490	arg2	sacrifice					519:527	sacrifice	519:527	sacrifice of strength and modulus of PLA	519:558	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	6	contain	have	487:490	arg2	use					561:563	use	561:563	use of toxic chemicals	561:582	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	6	contain	have	487:490	arg2	costs					593:597	high costs	588:597	high costs	588:597	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	6	contain	have	487:490	arg1	methods					479:485	these methods	473:485	these methods	473:485	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	10	7	theme	strong	1203:1208	arg1	skeletons					1222:1230	the strong but ductile skeletons	1199:1230	the strong but ductile skeletons of CS-g-oligo(D-LA)	1199:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	8	8	theme	CS-g-oligo	1011:1020	arg1	particles					1028:1036	CS-g-oligo(D-LA) particles	1011:1036	CS-g-oligo(D-LA) particles	1011:1036	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	8	9	theme	Compatibility	957:969	arg1	interactions					990:1001	Compatibility and intermolecular interactions	957:1001	interactions	990:1001	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	8	10	theme	D-LA	1022:1025	arg1	particles					1028:1036	CS-g-oligo(D-LA) particles	1011:1036	CS-g-oligo(D-LA) particles	1011:1036	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	11	11	theme	PLA-based	1505:1513	arg1	bio-composites					1515:1528	high-performance PLA-based bio-composites	1488:1528	high-performance PLA-based bio-composites	1488:1528	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	5	12	theme	poor	688:691	arg1	properties					704:713	poor mechanical properties	688:713	poor mechanical properties	688:713	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	7	13	theme	particles	904:912	arg1	effect					877:882	Reinforcing effect	865:882	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix	865:927	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	11	14	theme	strong	1386:1391	arg1	interactions					1393:1404	strong interactions	1386:1404	strong interactions between enantiomeric lactyl units	1386:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	10	15	theme	effective	1150:1158	arg1	transfer					1160:1167	effective transfer	1150:1167	effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA)	1150:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	5	16	theme	mechanical	693:702	arg1	properties					704:713	poor mechanical properties	688:713	poor mechanical properties	688:713	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	6	17	theme	PLLA	852:855	arg1	matrix					857:862	PLLA matrix	852:862	PLLA matrix	852:862	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	6	18	theme	CS-g-oligo	757:766	arg1	particles					774:782	CS-g-oligo(D-LA) particles	757:782	CS-g-oligo(D-LA) particles with good ductility	757:802	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	10	19	theme	CS-g-oligo	1235:1244	arg1	skeletons					1222:1230	the strong but ductile skeletons	1199:1230	the strong but ductile skeletons of CS-g-oligo(D-LA)	1199:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	11	20	theme	reinforcing	1348:1358	arg1	strategy					1360:1367	This novel reinforcing strategy	1337:1367	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units	1337:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	11	21	dep	fabrication	1457:1467	arg1	the					1453:1455	the	1453:1455	the	1453:1455	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	10	22	theme	stress	1172:1177	arg1	transfer					1160:1167	effective transfer	1150:1167	effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA)	1150:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	10	23	from	matrix	1189:1194	arg1	transfer					1160:1167	effective transfer	1150:1167	effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA)	1150:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	11	24	theme	novel	1342:1346	arg1	strategy					1360:1367	This novel reinforcing strategy	1337:1367	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units	1337:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	8	25	theme	PLLA	1042:1045	arg1	matrix					1047:1052	PLLA matrix	1042:1052	PLLA matrix	1042:1052	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	1	26	theme	improved	257:264	arg1	ductility					266:274	ductility	266:274	ductility	266:274	PLA bio-composites reinforced by oligo(d-lactic acid) grafted chitosan has been developed for simultaneously improved ductility, strength and modulus.
30890341	2	27	theme	Brittleness	299:309	arg1	problem					311:317	Brittleness problem	299:317	Brittleness problem	299:317	Brittleness problem greatly limits the applications of PLA, a polymer derived from corn.
30890341	0	28	theme	Poly	0:3	arg1	bio-composites					20:33	Poly(l-lactic acid) bio-composites	0:33	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid)	0:68	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	4	29	theme	strength	532:539	arg1	use					561:563	use	561:563	use of toxic chemicals	561:582	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	29	theme	strength	532:539	arg1	sacrifice					519:527	sacrifice	519:527	sacrifice of strength and modulus of PLA	519:558	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	29	theme	strength	532:539	arg1	costs					593:597	high costs	588:597	high costs	588:597	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	5	30	theme	Bio-based	600:608	arg1	elastomers					610:619	Bio-based elastomers	600:619	Bio-based elastomers such as chitosan	600:636	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	5	30	theme	Bio-based	600:608	arg1	chitosan					629:636	chitosan	629:636	chitosan	629:636	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	7	31	theme	CS-g-oligo	887:896	arg1	D-LA					898:901	D-LA	898:901	D-LA	898:901	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	7	31	theme	CS-g-oligo	887:896	arg1	particles					904:912	CS-g-oligo(D-LA) particles	887:912	CS-g-oligo(D-LA) particles	887:912	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	11	32	theme	lactyl	1427:1432	arg1	units					1434:1438	enantiomeric lactyl units	1414:1438	enantiomeric lactyl units	1414:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	0	33	theme	improved	106:113	arg1	ductility					115:123	simultaneously improved ductility	91:123	simultaneously improved ductility	91:123	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	8	34	theme	intermolecular	975:988	arg1	interactions					990:1001	Compatibility and intermolecular interactions	957:1001	interactions	990:1001	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	7	35	from	effect	877:882	arg1	matrix					922:927	PLLA matrix	917:927	PLLA matrix	917:927	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	3	36	theme	brittleness	437:447	arg1	problem					449:455	the brittleness problem	433:455	the brittleness problem	433:455	Various methods have been developed to solve the brittleness problem.
30890341	0	37	theme	l-lactic	5:12	arg1	Poly					0:3	Poly	0:3	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid)	0:68	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	0	37	theme	l-lactic	5:12	arg1	acid					14:17	l-lactic acid	5:17	l-lactic acid	5:17	Poly(l-lactic acid) bio-composites reinforced by oligo(d-lactic acid) grafted chitosan for simultaneously improved ductility, strength and modulus.
30890341	4	38	theme	modulus	545:551	arg1	use					561:563	use	561:563	use of toxic chemicals	561:582	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	38	theme	modulus	545:551	arg1	sacrifice					519:527	sacrifice	519:527	sacrifice of strength and modulus of PLA	519:558	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	38	theme	modulus	545:551	arg1	costs					593:597	high costs	588:597	high costs	588:597	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	6	39	theme	interfacial	822:832	arg1	interactions					834:845	strong interfacial interactions	815:845	strong interfacial interactions	815:845	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	4	40	theme	high	588:591	arg1	costs					593:597	high costs	588:597	high costs	588:597	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	3	41	theme	Various	388:394	arg1	methods					396:402	Various methods	388:402	Various methods	388:402	Various methods have been developed to solve the brittleness problem.
30890341	11	42	theme	interactions	1393:1404	arg1	formation					1373:1381	formation	1373:1381	formation of strong interactions between enantiomeric lactyl units	1373:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	6	43	theme	strong	815:820	arg1	interactions					834:845	strong interfacial interactions	815:845	strong interfacial interactions	815:845	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	11	44	theme	enantiomeric	1414:1425	arg1	units					1434:1438	enantiomeric lactyl units	1414:1438	enantiomeric lactyl units	1414:1438	This novel reinforcing strategy via formation of strong interactions between enantiomeric lactyl units would enrich the fabrication and exploration of high-performance PLA-based bio-composites.
30890341	1	45	theme	d-lactic	187:194	arg1	oligo					181:185	oligo	181:185	oligo	181:185	PLA bio-composites reinforced by oligo(d-lactic acid) grafted chitosan has been developed for simultaneously improved ductility, strength and modulus.
30890341	1	45	theme	d-lactic	187:194	arg1	acid					196:199	d-lactic acid	187:199	d-lactic acid	187:199	PLA bio-composites reinforced by oligo(d-lactic acid) grafted chitosan has been developed for simultaneously improved ductility, strength and modulus.
30890341	5	46	contain	have	643:646	arg2	compatibility					653:665	poor compatibility	648:665	poor compatibility	648:665	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	5	46	contain	have	643:646	arg1	elastomers					610:619	Bio-based elastomers	600:619	Bio-based elastomers such as chitosan	600:636	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	5	46	contain	have	643:646	arg1	chitosan					629:636	chitosan	629:636	chitosan	629:636	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	8	47	theme	SEM	1070:1072	arg1	analyses					1091:1098	SEM, DSC and 13C NMR analyses	1070:1098	SEM, DSC and 13C NMR analyses	1070:1098	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	2	48	attach	derived	369:375	arg2	PLA					354:356	PLA	354:356	PLA	354:356	Brittleness problem greatly limits the applications of PLA, a polymer derived from corn.
30890341	2	48	attach	derived	369:375	arg1	corn					382:385	corn	382:385	corn	382:385	Brittleness problem greatly limits the applications of PLA, a polymer derived from corn.
30890341	2	48	attach	derived	369:375	arg2	polymer					361:367	a polymer	359:367	a polymer derived from corn	359:385	Brittleness problem greatly limits the applications of PLA, a polymer derived from corn.
30890341	1	49	dep	bio-composites	152:165	arg1	reinforced					167:176	reinforced	167:176	bio-composites reinforced by oligo(d-lactic acid) grafted chitosan has been developed for simultaneously improved ductility, strength and modulus	152:296	PLA bio-composites reinforced by oligo(d-lactic acid) grafted chitosan has been developed for simultaneously improved ductility, strength and modulus.
30890341	5	50	theme	poor	648:651	arg1	compatibility					653:665	poor compatibility	648:665	poor compatibility	648:665	Bio-based elastomers such as chitosan also have poor compatibility with PLA, leading to poor mechanical properties.
30890341	6	51	theme	good	789:792	arg1	ductility					794:802	good ductility	789:802	good ductility	789:802	The hypothesis for this research is that CS-g-oligo(D-LA) particles with good ductility could form strong interfacial interactions with PLLA matrix.
30890341	4	52	theme	toxic	568:572	arg1	chemicals					574:582	toxic chemicals	568:582	toxic chemicals	568:582	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	8	53	theme	DSC	1075:1077	arg1	analyses					1091:1098	SEM, DSC and 13C NMR analyses	1070:1098	SEM, DSC and 13C NMR analyses	1070:1098	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	10	54	theme	PLLA	1184:1187	arg1	matrix					1189:1194	PLLA matrix	1184:1194	PLLA matrix	1184:1194	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	8	55	theme	NMR	1087:1089	arg1	analyses					1091:1098	SEM, DSC and 13C NMR analyses	1070:1098	SEM, DSC and 13C NMR analyses	1070:1098	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	10	56	theme	bio-composites	1293:1306	arg1	modulus					1277:1283	modulus	1277:1283	modulus	1277:1283	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	10	56	theme	bio-composites	1293:1306	arg1	strength					1264:1271	strength	1264:1271	strength	1264:1271	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	10	56	theme	bio-composites	1293:1306	arg1	ductility					1253:1261	ductility	1253:1261	ductility	1253:1261	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	9	57	theme	reinforcing	1105:1115	arg1	mechanism					1117:1125	The reinforcing mechanism	1101:1125	The reinforcing mechanism	1101:1125	The reinforcing mechanism was summarized.
30890341	7	58	theme	Reinforcing	865:875	arg1	effect					877:882	Reinforcing effect	865:882	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix	865:927	Reinforcing effect of CS-g-oligo(D-LA) particles on PLLA matrix was systematically studied.
30890341	8	59	theme	13C	1083:1085	arg1	NMR					1087:1089	13C NMR	1083:1089	13C NMR	1083:1089	Compatibility and intermolecular interactions between CS-g-oligo(D-LA) particles and PLLA matrix were studied by SEM, DSC and 13C NMR analyses.
30890341	10	60	theme	PLLA	1288:1291	arg1	bio-composites					1293:1306	PLLA bio-composites	1288:1306	PLLA bio-composites	1288:1306	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	2	61	theme	PLA	354:356	arg1	applications					338:349	the applications	334:349	the applications of PLA, a polymer derived from corn	334:385	Brittleness problem greatly limits the applications of PLA, a polymer derived from corn.
30890341	10	62	theme	ductile	1214:1220	arg1	skeletons					1222:1230	the strong but ductile skeletons	1199:1230	the strong but ductile skeletons of CS-g-oligo(D-LA)	1199:1250	Due to effective transfer of stress from PLLA matrix to the strong but ductile skeletons of CS-g-oligo(D-LA), ductility, strength and modulus of PLLA bio-composites were substantially improved.
30890341	4	63	theme	chemicals	574:582	arg1	use					561:563	use	561:563	use of toxic chemicals	561:582	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	63	theme	chemicals	574:582	arg1	sacrifice					519:527	sacrifice	519:527	sacrifice of strength and modulus of PLA	519:558	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
30890341	4	63	theme	chemicals	574:582	arg1	costs					593:597	high costs	588:597	high costs	588:597	Unfortunately, these methods have their limitations, such as sacrifice of strength and modulus of PLA, use of toxic chemicals and high costs.
29661299	1	0	theme	wide	174:177	arg1	range					179:183	a wide range	172:183	a wide range of medical purposes	172:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	4	1	theme	freeze	606:611	arg1	mode					613:616	freeze mode	606:616	freeze mode	606:616	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	2	2	with	acetate	415:421	arg1	collagen					428:435	collagen	428:435	collagen	428:435	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	2	with	acetate	415:421	arg1	chitosan					440:447	chitosan	440:447	chitosan	440:447	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	6	3	theme	chitosan	847:854	arg1	impregnation					831:842	The impregnation	827:842	The impregnation of chitosan or collagen filler in fiber matrix	827:889	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	3	4	theme	freeze-drying	525:537	arg1	electrospinning					495:509	electrospinning	495:509	electrospinning	495:509	The fibrous component was prepared by electrospinning, the sponge - freeze-drying technique.
29661299	3	4	theme	freeze-drying	525:537	arg1	technique					539:547	freeze-drying technique	525:547	freeze-drying technique	525:547	The fibrous component was prepared by electrospinning, the sponge - freeze-drying technique.
29661299	5	5	theme	different	751:759	arg1	types					761:765	different types	751:765	different types of microstructure	751:783	It was shown that scaffolds with different types of microstructure can be obtained employing this technique.
29661299	2	6	theme	tissue	305:310	arg1	architecture					267:278	the architecture	263:278	the architecture of native decellularized tissue	263:310	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	7	theme	decellularized	290:303	arg1	tissue					305:310	native decellularized tissue	283:310	native decellularized tissue	283:310	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	6	8	from	impregnation	831:842	arg1	matrix					884:889	fiber matrix	878:889	fiber matrix	878:889	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	6	9	theme	mechanical	919:928	arg1	properties					930:939	mechanical properties	919:939	mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus	919:997	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	6	10	dep	40 MPa	947:952	arg1	up					941:942	up	941:942	up	941:942	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	2	11	theme	native	283:288	arg1	tissue					305:310	native decellularized tissue	283:310	native decellularized tissue	283:310	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	1	12	theme	medical	188:194	arg1	purposes					196:203	medical purposes	188:203	medical purposes	188:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	0	13	theme	tissue-engineering	19:36	arg1	scaffolds					47:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	4	14	theme	fiber-sponge	663:674	arg1	architecture					676:687	the fiber-sponge architecture	659:687	the fiber-sponge architecture	659:687	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	1	15	theme	purposes	196:203	arg1	range					179:183	a wide range	172:183	a wide range of medical purposes	172:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	0	16	theme	Multi-hierarchical	0:17	arg1	scaffolds					47:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	4	17	theme	parameters	586:595	arg1	influence					554:562	The influence	550:562	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties	550:702	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	18	theme	polymer	619:625	arg1	mode					613:616	freeze mode	606:616	freeze mode	606:616	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	18	theme	polymer	619:625	arg1	type					627:630	polymer type	619:630	polymer type	619:630	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	18	theme	polymer	619:625	arg1	parameters					586:595	main technological parameters	567:595	main technological parameters	567:595	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	19	theme	technological	572:584	arg1	mode					613:616	freeze mode	606:616	freeze mode	606:616	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	19	theme	technological	572:584	arg1	parameters					586:595	main technological parameters	567:595	main technological parameters	567:595	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	19	theme	technological	572:584	arg1	type					627:630	polymer type	619:630	polymer type	619:630	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	19	theme	technological	572:584	arg1	etc.					651:654	etc.	651:654	etc.	651:654	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	19	theme	technological	572:584	arg1	concentration					636:648	concentration	636:648	concentration	636:648	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	0	20	dep	collagen	89:96	arg1	fillers					111:117	fillers	111:117	fillers	111:117	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	0	21	theme	ECM-like	38:45	arg1	scaffolds					47:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds	0:55	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	1	22	theme	novel	122:126	arg1	matrixes					159:166	A novel high-tech composite biomimetic matrixes	120:166	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes	120:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	6	23	dep	chitosan	847:854	arg1	filler					868:873	filler	868:873	filler	868:873	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	5	24	with	scaffolds	736:744	arg1	types					761:765	different types	751:765	different types of microstructure	751:783	It was shown that scaffolds with different types of microstructure can be obtained employing this technique.
29661299	2	25	theme	fibrous	347:353	arg1	components					355:364	fibrous components	347:364	fibrous components of different spatial geometry	347:394	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	26	dep	collagen	428:435	arg1	filler					449:454	filler	449:454	filler	449:454	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	27	theme	different	369:377	arg1	geometry					387:394	different spatial geometry	369:394	different spatial geometry	369:394	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	4	28	from	influence	554:562	arg1	architecture					676:687	the fiber-sponge architecture	659:687	the fiber-sponge architecture	659:687	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	28	from	influence	554:562	arg1	properties					693:702	properties	693:702	properties	693:702	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	6	29	theme	Young	983:987	arg1	modulus					991:997	Young's modulus	983:997	Young's modulus	983:997	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	6	30	theme	fiber	878:882	arg1	matrix					884:889	fiber matrix	878:889	fiber matrix	878:889	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	2	31	theme	scaffolds	237:245	arg1	structure					224:232	The structure	220:232	The structure of scaffolds	220:245	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	1	32	theme	high-tech	128:136	arg1	matrixes					159:166	A novel high-tech composite biomimetic matrixes	120:166	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes	120:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	0	33	theme	cellulose	66:74	arg1	acetate					76:82	cellulose acetate	66:82	cellulose acetate with collagen and chitosan fillers	66:117	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	4	34	theme	main	567:570	arg1	mode					613:616	freeze mode	606:616	freeze mode	606:616	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	34	theme	main	567:570	arg1	parameters					586:595	main technological parameters	567:595	main technological parameters	567:595	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	34	theme	main	567:570	arg1	type					627:630	polymer type	619:630	polymer type	619:630	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	34	theme	main	567:570	arg1	etc.					651:654	etc.	651:654	etc.	651:654	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	4	34	theme	main	567:570	arg1	concentration					636:648	concentration	636:648	concentration	636:648	The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined.
29661299	1	35	theme	composite	138:146	arg1	matrixes					159:166	A novel high-tech composite biomimetic matrixes	120:166	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes	120:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	1	36	theme	biomimetic	148:157	arg1	matrixes					159:166	A novel high-tech composite biomimetic matrixes	120:166	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes	120:203	A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared.
29661299	6	37	theme	collagen	859:866	arg1	impregnation					831:842	The impregnation	827:842	The impregnation of chitosan or collagen filler in fiber matrix	827:889	The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.
29661299	0	38	with	acetate	76:82	arg1	chitosan					102:109	chitosan	102:109	chitosan	102:109	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	0	38	with	acetate	76:82	arg1	collagen					89:96	collagen	89:96	collagen	89:96	Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers.
29661299	3	39	theme	fibrous	461:467	arg1	component					469:477	The fibrous component	457:477	The fibrous component	457:477	The fibrous component was prepared by electrospinning, the sponge - freeze-drying technique.
29661299	5	40	theme	microstructure	770:783	arg1	types					761:765	different types	751:765	different types of microstructure	751:783	It was shown that scaffolds with different types of microstructure can be obtained employing this technique.
29661299	2	41	theme	geometry	387:394	arg1	sponge					336:341	a sponge	334:341	a sponge	334:341	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	41	theme	geometry	387:394	arg1	components					355:364	fibrous components	347:364	fibrous components of different spatial geometry	347:394	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	42	theme	cellulose	405:413	arg1	acetate					415:421	cellulose acetate	405:421	cellulose acetate with collagen or chitosan filler	405:454	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
29661299	2	43	theme	spatial	379:385	arg1	geometry					387:394	different spatial geometry	369:394	different spatial geometry	369:394	The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler.
31870828	3	0	from	lactose	365:371	arg1	ratios					386:391	different ratios	376:391	different ratios with and without HPMC	376:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	1	1	theme	common	136:141	arg1	issue					143:147	the common issue	132:147	the common issue of weak interfacial bonding strength (IBS) with manufacturing	132:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	2	2	from	effects	265:271	arg1	layers					302:307	individual layers	291:307	individual layers	291:307	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	2	2	from	effects	265:271	arg1	pressure					324:331	compaction pressure	313:331	compaction pressure	313:331	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	2	2	from	effects	265:271	arg1	IBS					340:342	the IBS	336:342	the IBS	336:342	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	4	3	theme	bonding	778:784	arg1	strength					786:793	particle bonding strength	769:793	particle bonding strength	769:793	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	2	4	from	layers	302:307	arg1	effects					265:271	the effects	261:271	the effects of composition in individual layers and compaction pressure on the IBS	261:342	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	3	5	dep	pressures	480:488	arg1	20					491:492	20	491:492	20	491:492	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	3	5	dep	pressures	480:488	arg1	100 MPa					498:504	100 MPa	498:504	100 MPa	498:504	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	4	6	theme	bonding	799:805	arg1	area					807:810	bonding area	799:810	bonding area	799:810	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	4	7	theme	IBS	684:686	arg1	trends					659:664	the complex trends	647:664	the complex trends of bilayer tablet IBS as a function of MCC content	647:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	3	8	used	used	420:423	arg2	Mixtures					345:352	Mixtures	345:352	Mixtures of MCC and lactose in different ratios with and without HPMC	345:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	4	9	theme	tablet	677:682	arg1	IBS					684:686	bilayer tablet IBS	669:686	bilayer tablet IBS as a function of MCC content	669:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	1	10	theme	full	59:62	arg1	advantage					64:72	full advantage	59:72	full advantage of the drug delivery benefits offered by bilayer tablets	59:129	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	3	11	theme	first	435:439	arg1	layer					441:445	the first layer	431:445	the first layer	431:445	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	3	12	from	Mixtures	345:352	arg1	ratios					386:391	different ratios	376:391	different ratios with and without HPMC	376:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	1	13	theme	weak	152:155	arg1	IBS					187:189	IBS	187:189	IBS	187:189	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	13	theme	weak	152:155	arg1	strength					177:184	weak interfacial bonding strength	152:184	weak interfacial bonding strength (IBS) with manufacturing	152:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	3	14	from	MCC	357:359	arg1	ratios					386:391	different ratios	376:391	different ratios with and without HPMC	376:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	4	15	theme	particle	769:776	arg1	strength					786:793	particle bonding strength	769:793	particle bonding strength	769:793	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	2	16	from	pressure	324:331	arg1	effects					265:271	the effects	261:271	the effects of composition in individual layers and compaction pressure on the IBS	261:342	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	1	17	theme	interfacial	157:167	arg1	IBS					187:189	IBS	187:189	IBS	187:189	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	17	theme	interfacial	157:167	arg1	strength					177:184	weak interfacial bonding strength	152:184	weak interfacial bonding strength (IBS) with manufacturing	152:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	3	18	theme	MCC	357:359	arg1	Mixtures					345:352	Mixtures	345:352	Mixtures of MCC and lactose in different ratios with and without HPMC	345:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	1	19	theme	drug	81:84	arg1	benefits					95:102	the drug delivery benefits	77:102	the drug delivery benefits offered by bilayer tablets	77:129	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	20	theme	bonding	169:175	arg1	IBS					187:189	IBS	187:189	IBS	187:189	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	20	theme	bonding	169:175	arg1	strength					177:184	weak interfacial bonding strength	152:184	weak interfacial bonding strength (IBS) with manufacturing	152:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	0	21	theme	formulated	23:32	arg1	tablets					42:48	formulated bilayer tablets	23:48	formulated bilayer tablets	23:48	Interfacial bonding in formulated bilayer tablets.
31870828	4	22	theme	complex	651:657	arg1	trends					659:664	the complex trends	647:664	the complex trends of bilayer tablet IBS as a function of MCC content	647:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	1	23	theme	delivery	86:93	arg1	benefits					95:102	the drug delivery benefits	77:102	the drug delivery benefits offered by bilayer tablets	77:129	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	24	theme	strength	177:184	arg1	issue					143:147	the common issue	132:147	the common issue of weak interfacial bonding strength (IBS) with manufacturing	132:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	2	25	theme	composition	276:286	arg1	effects					265:271	the effects	261:271	the effects of composition in individual layers and compaction pressure on the IBS	261:342	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	1	26	theme	benefits	95:102	arg1	advantage					64:72	full advantage	59:72	full advantage of the drug delivery benefits offered by bilayer tablets	59:129	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	1	27	with	strength	177:184	arg1	manufacturing					197:209	manufacturing	197:209	manufacturing	197:209	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	3	28	theme	layer	528:532	arg1	pressure					545:552	a second layer compaction pressure	519:552	a second layer compaction pressure of 200 MPa	519:563	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	4	29	theme	failure	588:594	arg1	mode					596:599	the failure mode	584:599	the failure mode	584:599	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	0	30	theme	bilayer	34:40	arg1	tablets					42:48	formulated bilayer tablets	23:48	formulated bilayer tablets	23:48	Interfacial bonding in formulated bilayer tablets.
31870828	3	31	theme	different	376:384	arg1	ratios					386:391	different ratios	376:391	different ratios with and without HPMC	376:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	3	32	theme	compaction	534:543	arg1	pressure					545:552	a second layer compaction pressure	519:552	a second layer compaction pressure of 200 MPa	519:563	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	2	33	theme	compaction	313:322	arg1	pressure					324:331	compaction pressure	313:331	compaction pressure	313:331	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	3	34	theme	lactose	365:371	arg1	Mixtures					345:352	Mixtures	345:352	Mixtures of MCC and lactose in different ratios with and without HPMC	345:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	3	35	theme	second	521:526	arg1	pressure					545:552	a second layer compaction pressure	519:552	a second layer compaction pressure of 200 MPa	519:563	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	3	36	theme	200 MPa	557:563	arg1	pressure					545:552	a second layer compaction pressure	519:552	a second layer compaction pressure of 200 MPa	519:563	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	1	37	theme	bilayer	115:121	arg1	tablets					123:129	bilayer tablets	115:129	bilayer tablets	115:129	To take full advantage of the drug delivery benefits offered by bilayer tablets, the common issue of weak interfacial bonding strength (IBS) with manufacturing must be overcome.
31870828	4	38	theme	bilayer	669:675	arg1	IBS					684:686	bilayer tablet IBS	669:686	bilayer tablet IBS as a function of MCC content	669:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	3	39	from	ratios	386:391	arg1	Mixtures					345:352	Mixtures	345:352	Mixtures of MCC and lactose in different ratios with and without HPMC	345:413	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31870828	2	40	theme	individual	291:300	arg1	layers					302:307	individual layers	291:307	individual layers	291:307	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	2	41	from	composition	276:286	arg1	layers					302:307	individual layers	291:307	individual layers	291:307	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	2	41	from	composition	276:286	arg1	pressure					324:331	compaction pressure	313:331	compaction pressure	313:331	This work seeks to characterize the effects of composition in individual layers and compaction pressure on the IBS.
31870828	4	42	theme	content	709:715	arg1	function					693:700	a function	691:700	a function of MCC content	691:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	4	43	theme	MCC	705:707	arg1	content					709:715	MCC content	705:715	MCC content	705:715	After identifying the failure mode as either at the interface or within a layer, the complex trends of bilayer tablet IBS as a function of MCC content were explained by considering the interplay between particle bonding strength and bonding area at the interface.
31870828	3	44	theme	different	470:478	arg1	pressures					480:488	two different pressures	466:488	two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa	466:563	Mixtures of MCC and lactose in different ratios with and without HPMC were used where the first layer was compacted with two different pressures (20 and 100 MPa) followed by a second layer compaction pressure of 200 MPa.
31004953	5	0	theme	mechanical	911:920	arg1	performance					922:932	prominent mechanical performance	901:932	prominent mechanical performance due to strong adhesion of fragments and matrix	901:979	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	0	1	theme	tetrabutylammonium	79:96	arg1	fluoride					98:105	tetrabutylammonium fluoride	79:105	tetrabutylammonium fluoride	79:105	Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.
31004953	3	2	theme	fibrillated	704:714	arg1	fragments					716:724	fibrillated fragments	704:724	fibrillated fragments	704:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	3	3	theme	higher	531:536	arg1	efficiency					538:547	higher efficiency	531:547	higher efficiency	531:547	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	6	4	theme	biomass	1097:1103	arg1	conversion					1061:1070	efficient conversion	1051:1070	efficient conversion of waste lignocellulosic biomass	1051:1103	This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
31004953	3	5	theme	fragments	716:724	arg1	compaction					690:699	the compaction	686:699	the compaction of fibrillated fragments	686:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	4	6	theme	succinic	804:811	arg1	anhydride					813:821	succinic anhydride	804:821	succinic anhydride	804:821	Pre-ball-milling of MW for 4.0 h apparently promoted the esterification with succinic anhydride.
31004953	2	7	dep	water	355:359	arg1	fluoride					384:391	fluoride	384:391	fluoride	384:391	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	6	8	theme	lignocellulosic	1081:1095	arg1	biomass					1097:1103	waste lignocellulosic biomass	1075:1103	waste lignocellulosic biomass	1075:1103	This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
31004953	6	9	theme	waste	1075:1079	arg1	biomass					1097:1103	waste lignocellulosic biomass	1075:1103	waste lignocellulosic biomass	1075:1103	This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
31004953	4	10	theme	MW	747:748	arg1	Pre-ball-milling					727:742	Pre-ball-milling	727:742	Pre-ball-milling of MW for 4.0 h	727:758	Pre-ball-milling of MW for 4.0 h apparently promoted the esterification with succinic anhydride.
31004953	0	11	with	Ball-milling	61:72	arg1	fluoride					98:105	tetrabutylammonium fluoride	79:105	tetrabutylammonium fluoride	79:105	Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.
31004953	3	12	theme	same	450:453	arg1	condition					455:463	the same condition	446:463	the same condition	446:463	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	2	13	theme	all-wood-plastic	411:426	arg1	composites					428:437	all-wood-plastic composites	411:437	all-wood-plastic composites	411:437	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	3	14	dep	meanwhile	626:634	arg1	hindered					677:684	hindered	677:684	hindered the compaction of fibrillated fragments	677:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	3	15	with	ball-milling	498:509	arg1	TBAF					516:519	TBAF	516:519	TBAF	516:519	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	1	16	theme	lignocellulosic	208:222	arg1	matters					224:230	lignocellulosic matters	208:230	lignocellulosic matters	208:230	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	5	17	theme	7.0 h	867:871	arg1	succinoylation					873:886	7.0 h succinoylation	867:886	7.0 h succinoylation	867:886	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	2	18	theme	wet	333:335	arg1	ball-milling					337:348	wet ball-milling	333:348	wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites	333:437	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	1	19	theme	matters	224:230	arg1	agglomeration					191:203	agglomeration	191:203	agglomeration of lignocellulosic matters	191:230	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	0	20	theme	Mechanochemical	0:14	arg1	esterification					16:29	Mechanochemical esterification	0:29	Mechanochemical esterification of waste	0:38	Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.
31004953	3	21	theme	catalytic	596:604	arg1	function					606:613	catalytic function	596:613	catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments	596:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	5	22	theme	fragments	960:968	arg1	adhesion					948:955	strong adhesion	941:955	strong adhesion of fragments and matrix	941:979	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	0	23	theme	waste	34:38	arg1	esterification					16:29	Mechanochemical esterification	0:29	Mechanochemical esterification of waste	0:38	Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.
31004953	3	24	theme	meanwhile	626:634	arg1	function					606:613	catalytic function	596:613	catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments	596:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	3	25	theme	MW	488:489	arg1	esterification					470:483	the esterification	466:483	the esterification of MW by wet ball-milling with TBAF	466:519	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	5	26	theme	prominent	901:909	arg1	performance					922:932	prominent mechanical performance	901:932	prominent mechanical performance due to strong adhesion of fragments and matrix	901:979	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	6	27	theme	efficient	1051:1059	arg1	conversion					1061:1070	efficient conversion	1051:1070	efficient conversion of waste lignocellulosic biomass	1051:1103	This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
31004953	3	28	theme	cellulose	659:667	arg1	fibrils					669:675	cellulose fibrils	659:675	cellulose fibrils	659:675	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	1	29	theme	lignocellulosic	126:140	arg1	biomass					142:148	lignocellulosic biomass	126:148	lignocellulosic biomass driven by dry ball-milling	126:175	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	5	30	theme	All-wood-plastic	824:839	arg1	composites					841:850	All-wood-plastic composites	824:850	All-wood-plastic composites prepared after 7.0 h succinoylation	824:886	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	2	31	with	ball-milling	337:348	arg1	water					355:359	water	355:359	water	355:359	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	2	31	with	ball-milling	337:348	arg1	tetrabutylammonium					365:382	tetrabutylammonium	365:382	tetrabutylammonium	365:382	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	2	31	with	ball-milling	337:348	arg1	TBAF					394:397	TBAF	394:397	TBAF	394:397	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	3	32	theme	F-	618:619	arg1	ions					621:624	F- ions	618:624	F- ions	618:624	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	5	33	theme	strong	941:946	arg1	adhesion					948:955	strong adhesion	941:955	strong adhesion of fragments and matrix	941:979	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31004953	4	34	with	esterification	784:797	arg1	anhydride					813:821	succinic anhydride	804:821	succinic anhydride	804:821	Pre-ball-milling of MW for 4.0 h apparently promoted the esterification with succinic anhydride.
31004953	3	35	theme	TBA+	651:654	arg1	binding					640:646	the binding	636:646	the binding of TBA+ to cellulose fibrils	636:675	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	3	36	theme	ions	621:624	arg1	function					606:613	catalytic function	596:613	catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments	596:724	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	0	37	theme	wet	57:59	arg1	Ball-milling					61:72	wet Ball-milling	57:72	wet Ball-milling with tetrabutylammonium fluoride	57:105	Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.
31004953	6	38	theme	environment-friendly	1019:1038	arg1	method					1040:1045	an environment-friendly method	1016:1045	an environment-friendly method for efficient conversion of waste lignocellulosic biomass	1016:1103	This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
31004953	1	39	theme	biomass	142:148	arg1	Esterification					108:121	Esterification	108:121	Esterification of lignocellulosic biomass driven by dry ball-milling	108:175	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	2	40	theme	wood	304:307	arg1	esterification					271:284	esterification	271:284	esterification of waste mulberry wood (MW)	271:312	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	3	41	theme	wet	494:496	arg1	ball-milling					498:509	wet ball-milling	494:509	wet ball-milling with TBAF	494:519	Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F- ions meanwhile the binding of TBA+ to cellulose fibrils hindered the compaction of fibrillated fragments.
31004953	2	42	theme	mulberry	295:302	arg1	MW					310:311	MW	310:311	MW	310:311	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	2	42	theme	mulberry	295:302	arg1	wood					304:307	waste mulberry wood	289:307	waste mulberry wood (MW)	289:312	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	1	43	theme	dry	160:162	arg1	ball-milling					164:175	dry ball-milling	160:175	dry ball-milling	160:175	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	2	44	theme	waste	289:293	arg1	MW					310:311	MW	310:311	MW	310:311	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	2	44	theme	waste	289:293	arg1	wood					304:307	waste mulberry wood	289:307	waste mulberry wood (MW)	289:312	In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites.
31004953	1	45	theme	milling	239:245	arg1	process					247:253	milling process	239:253	milling process	239:253	Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process.
31004953	5	46	theme	matrix	974:979	arg1	adhesion					948:955	strong adhesion	941:955	strong adhesion of fragments and matrix	941:979	All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix.
31762457	3	0	theme	high-performance	498:513	arg1	assessment					576:585	an assessment	573:585	an assessment of their advantages and limitations	573:621	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	0	theme	high-performance	498:513	arg1	composites					525:534	high-performance cellulose composites	498:534	high-performance cellulose composites	498:534	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	8	1	theme	Vacuum	976:981	arg1	densification					983:995	Vacuum densification	976:995	Vacuum densification in an open-mold process	976:1019	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	3	2	theme	cellulose	515:523	arg1	assessment					576:585	an assessment	573:585	an assessment of their advantages and limitations	573:621	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	2	theme	cellulose	515:523	arg1	composites					525:534	high-performance cellulose composites	498:534	high-performance cellulose composites	498:534	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	1	3	theme	excellent	252:260	arg1	properties					273:282	its excellent mechanical properties	248:282	its excellent mechanical properties	248:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	5	4	theme	Closed-mold	733:743	arg1	densification					745:757	Closed-mold densification	733:757	Closed-mold densification	733:757	Closed-mold densification has the advantage that no elaborate lab equipment is needed.
31762457	7	5	theme	curvature	965:973	arg1	geometries					935:944	simple geometries	928:944	simple geometries	928:944	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	7	5	theme	curvature	965:973	arg1	radii					956:960	large radii	950:960	large radii of curvature	950:973	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	2	6	from	fragile	322:328	arg1	state					339:343	a wet state	333:343	a wet state	333:343	Delignified wood, however, is rather fragile in a wet state, which makes handling and shaping challenging.
31762457	1	7	theme	Delignified	66:76	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	7	theme	Delignified	66:76	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	8	theme	mechanical	262:271	arg1	properties					273:282	its excellent mechanical properties	248:282	its excellent mechanical properties	248:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	8	9	theme	larger	1037:1042	arg1	radii					1092:1096	small radii	1086:1096	small radii of curvature	1086:1109	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	8	9	theme	larger	1037:1042	arg1	objects					1044:1050	larger objects	1037:1050	larger objects	1037:1050	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	6	10	theme	Simple	820:825	arg1	clamps					833:838	Simple screw clamps	820:838	Simple screw clamps	820:838	Simple screw clamps or a press can be used for densification.
31762457	1	11	theme	densified	78:86	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	11	theme	densified	78:86	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	12	theme	sustainable	116:126	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	12	theme	sustainable	116:126	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	8	13	theme	complex	1056:1062	arg1	radii					1092:1096	small radii	1086:1096	small radii of curvature	1086:1109	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	8	13	theme	complex	1056:1062	arg1	geometries					1064:1073	complex geometries	1056:1073	complex geometries	1056:1073	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	3	14	theme	vacuum	465:470	arg1	processes					424:432	two fabrication processes	408:432	two fabrication processes	408:432	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	14	theme	vacuum	465:470	arg1	densification					472:484	vacuum densification	465:484	vacuum densification	465:484	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	2	15	theme	Delignified	285:295	arg1	wood					297:300	Delignified wood	285:300	Delignified wood	285:300	Delignified wood, however, is rather fragile in a wet state, which makes handling and shaping challenging.
31762457	1	16	theme	synthetic	177:185	arg1	materials					187:195	synthetic materials	177:195	synthetic materials	177:195	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	5	17	theme	lab	795:797	arg1	equipment					799:807	no elaborate lab equipment	782:807	no elaborate lab equipment	782:807	Closed-mold densification has the advantage that no elaborate lab equipment is needed.
31762457	8	18	theme	open-mold	1003:1011	arg1	process					1013:1019	an open-mold process	1000:1019	an open-mold process	1000:1019	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	3	19	theme	delignified	545:555	arg1	wood					557:560	delignified wood	545:560	delignified wood	545:560	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	7	20	theme	large	950:954	arg1	radii					956:960	large radii	950:960	large radii of curvature	950:973	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	7	21	with	parts	917:921	arg1	geometries					935:944	simple geometries	928:944	simple geometries	928:944	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	7	21	with	parts	917:921	arg1	radii					956:960	large radii	950:960	large radii of curvature	950:973	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	2	22	from	state	339:343	arg1	fragile					322:328	fragile	322:328	fragile	322:328	Delignified wood, however, is rather fragile in a wet state, which makes handling and shaping challenging.
31762457	5	23	contain	has	759:761	arg1	densification					745:757	Closed-mold densification	733:757	Closed-mold densification	733:757	Closed-mold densification has the advantage that no elaborate lab equipment is needed.
31762457	5	23	contain	has	759:761	arg2	advantage					767:775	the advantage that no elaborate lab equipment is needed	763:817	the advantage that no elaborate lab equipment is needed	763:817	Closed-mold densification has the advantage that no elaborate lab equipment is needed.
31762457	5	24	dep	equipment	799:807	arg1	elaborate					785:793	elaborate	785:793	elaborate	785:793	Closed-mold densification has the advantage that no elaborate lab equipment is needed.
31762457	9	25	theme	single	1211:1216	arg1	cavity					1223:1228	a single mold cavity	1209:1228	a single mold cavity	1209:1228	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
31762457	1	26	theme	new	98:100	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	26	theme	new	98:100	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	3	27	theme	fabrication	412:422	arg1	processes					424:432	two fabrication processes	408:432	two fabrication processes	408:432	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	27	theme	fabrication	412:422	arg1	densification					447:459	closed-mold densification	435:459	closed-mold densification	435:459	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	27	theme	fabrication	412:422	arg1	densification					472:484	vacuum densification	465:484	vacuum densification	465:484	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	8	28	theme	small	1086:1090	arg1	radii					1092:1096	small radii	1086:1096	small radii of curvature	1086:1109	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	7	29	theme	simple	928:933	arg1	geometries					935:944	simple geometries	928:944	simple geometries	928:944	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	1	30	theme	promising	102:110	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	30	theme	promising	102:110	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	9	31	theme	cavity	1223:1228	arg1	manufacture					1194:1204	the manufacture	1190:1204	the manufacture of a single mold cavity	1190:1228	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
31762457	1	32	theme	glass	206:210	arg1	composites					229:238	glass fiber reinforced composites	206:238	glass fiber reinforced composites	206:238	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	0	33	theme	High-Performance	37:52	arg1	Composites					54:63	Wood-Based High-Performance Composites	26:63	Wood-Based High-Performance Composites	26:63	Fabrication and Design of Wood-Based High-Performance Composites.
31762457	3	34	theme	closed-mold	435:445	arg1	processes					424:432	two fabrication processes	408:432	two fabrication processes	408:432	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	3	34	theme	closed-mold	435:445	arg1	densification					447:459	closed-mold densification	435:459	closed-mold densification	435:459	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	1	35	theme	fiber	212:216	arg1	composites					229:238	glass fiber reinforced composites	206:238	glass fiber reinforced composites	206:238	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	2	36	theme	challenging	379:389	arg1	shaping					371:377	shaping challenging	371:389	shaping challenging	371:389	Delignified wood, however, is rather fragile in a wet state, which makes handling and shaping challenging.
31762457	0	37	theme	Wood-Based	26:35	arg1	Composites					54:63	Wood-Based High-Performance Composites	26:63	Wood-Based High-Performance Composites	26:63	Fabrication and Design of Wood-Based High-Performance Composites.
31762457	3	38	theme	limitations	611:621	arg1	assessment					576:585	an assessment	573:585	an assessment of their advantages and limitations	573:621	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	1	39	theme	reinforced	218:227	arg1	composites					229:238	glass fiber reinforced composites	206:238	glass fiber reinforced composites	206:238	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	9	40	theme	closed-mold	1128:1138	arg1	process					1140:1146	the closed-mold process	1124:1146	the closed-mold process	1124:1146	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
31762457	2	41	theme	wet	335:337	arg1	state					339:343	a wet state	333:343	a wet state	333:343	Delignified wood, however, is rather fragile in a wet state, which makes handling and shaping challenging.
31762457	0	42	theme	Composites	54:63	arg1	Design					16:21	Design	16:21	Design	16:21	Fabrication and Design of Wood-Based High-Performance Composites.
31762457	0	42	theme	Composites	54:63	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Design of Wood-Based High-Performance Composites.
31762457	8	43	theme	curvature	1101:1109	arg1	radii					1092:1096	small radii	1086:1096	small radii of curvature	1086:1109	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	3	44	theme	advantages	596:605	arg1	assessment					576:585	an assessment	573:585	an assessment of their advantages and limitations	573:621	Here we present two fabrication processes, closed-mold densification and vacuum densification, to produce high-performance cellulose composites based on delignified wood, including an assessment of their advantages and limitations.
31762457	8	45	from	densification	983:995	arg1	process					1013:1019	an open-mold process	1000:1019	an open-mold process	1000:1019	Vacuum densification in an open-mold process is suitable for larger objects and complex geometries, including small radii of curvature.
31762457	7	46	theme	small	911:915	arg1	parts					917:921	small parts	911:921	small parts with simple geometries and large radii of curvature	911:973	We recommend this method for small parts with simple geometries and large radii of curvature.
31762457	9	47	theme	mold	1218:1221	arg1	cavity					1223:1228	a single mold cavity	1209:1228	a single mold cavity	1209:1228	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
31762457	4	48	theme	end-of-life	714:724	arg1	cycle					726:730	the end-of-life cycle	710:730	the end-of-life cycle	710:730	Further, we suggest strategies for how the composites can be re-used or decomposed at the end-of-life cycle.
31762457	6	49	theme	screw	827:831	arg1	clamps					833:838	Simple screw clamps	820:838	Simple screw clamps	820:838	Simple screw clamps or a press can be used for densification.
31762457	6	50	used	used	858:861	arg2	clamps					833:838	Simple screw clamps	820:838	Simple screw clamps	820:838	Simple screw clamps or a press can be used for densification.
31762457	6	50	used	used	858:861	arg2	press					845:849	a press	843:849	a press	843:849	Simple screw clamps or a press can be used for densification.
31762457	9	51	theme	open-mold	1153:1161	arg1	approach					1170:1177	the open-mold vacuum approach	1149:1177	the open-mold vacuum approach	1149:1177	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
31762457	1	52	contain	possesses	142:150	arg1	wood					88:91	Delignified densified wood	66:91	Delignified densified wood	66:91	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	52	contain	possesses	142:150	arg2	potential					156:164	the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	152:282	the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	152:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	1	52	contain	possesses	142:150	arg1	material					128:135	a new promising and sustainable material	96:135	a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties	96:282	Delignified densified wood is a new promising and sustainable material that possesses the potential to replace synthetic materials, such as glass fiber reinforced composites, due to its excellent mechanical properties.
31762457	9	53	theme	vacuum	1163:1168	arg1	approach					1170:1177	the open-mold vacuum approach	1149:1177	the open-mold vacuum approach	1149:1177	Compared to the closed-mold process, the open-mold vacuum approach only needs the manufacture of a single mold cavity.
29443907	8	0	with	comparison	1043:1052	arg1	films					1064:1068	HPMC films	1059:1068	HPMC films	1059:1068	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	10	1	from	days	1498:1501	arg1	°C					1519:1520	5 °C	1517:1520	5 °C	1517:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	2	theme	storage	1506:1512	arg1	days					1498:1501	the first five days	1483:1501	the first five days of storage at 5 °C	1483:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	7	3	dep	LAB	962:964	arg1	microbeads					985:994	microbeads	985:994	microbeads	985:994	Results showed that the addition of LAB or alginate-pectin microbeads modified slightly films optical properties.
29443907	3	4	theme	corn	376:379	arg1	starch					381:386	corn starch	376:386	corn starch	376:386	Two different biopolymers were tested: cellulose derivative (hydroxylpropylmethylcellulose (HPMC)) and corn starch.
29443907	3	5	theme	different	277:285	arg1	biopolymers					287:297	Two different biopolymers	273:297	Two different biopolymers	273:297	Two different biopolymers were tested: cellulose derivative (hydroxylpropylmethylcellulose (HPMC)) and corn starch.
29443907	4	6	theme	encapsulated	435:446	arg1	LAB					411:413	LAB	411:413	LAB	411:413	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	4	6	theme	encapsulated	435:446	arg1	bacteria					401:408	Lactic acid bacteria	389:408	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads	389:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	5	7	theme	vapour	696:701	arg1	permeability					703:714	the water vapour permeability	686:714	the water vapour permeability	686:714	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	5	8	theme	culture	650:656	arg1	incorporation					618:630	the incorporation	614:630	the incorporation of the protective culture into the biopolymer matrix	614:683	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	4	9	theme	forming	529:535	arg1	solution					537:544	the film forming solution	520:544	the film forming solution	520:544	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	10	10	theme	bioactive	1409:1417	arg1	culture					1419:1425	encapsulated bioactive culture	1396:1425	encapsulated bioactive culture	1396:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	4	11	theme	alginate-pectin	451:465	arg1	microbeads					486:495	alginate-pectin composite hydrogel microbeads	451:495	alginate-pectin composite hydrogel microbeads	451:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	5	12	theme	dry	779:781	arg1	films					783:787	the dry films	775:787	the dry films	775:787	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	4	13	theme	film	524:527	arg1	solution					537:544	the film forming solution	520:544	the film forming solution	520:544	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	7	14	theme	optical	1020:1026	arg1	properties					1028:1037	films optical properties	1014:1037	films optical properties	1014:1037	Results showed that the addition of LAB or alginate-pectin microbeads modified slightly films optical properties.
29443907	9	15	theme	alginate-pectin	1329:1343	arg1	beads					1345:1349	alginate-pectin beads	1329:1349	alginate-pectin beads	1329:1349	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	5	16	theme	films	783:787	arg1	permeability					724:735	oxygen permeability	717:735	oxygen permeability	717:735	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	5	16	theme	films	783:787	arg1	properties					761:770	mechanical properties	750:770	mechanical properties	750:770	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	5	16	theme	films	783:787	arg1	optical					738:744	optical	738:744	optical	738:744	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	5	16	theme	films	783:787	arg1	permeability					703:714	the water vapour permeability	686:714	the water vapour permeability	686:714	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	5	17	theme	biopolymer	667:676	arg1	matrix					678:683	the biopolymer matrix	663:683	the biopolymer matrix	663:683	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	2	18	theme	polysaccharide	251:264	arg1	films					266:270	polysaccharide films	251:270	polysaccharide films	251:270	Novel bioactive films were developed from the incorporation of Lactococcus lactis into polysaccharide films.
29443907	7	19	theme	films	1014:1018	arg1	properties					1028:1037	films optical properties	1014:1037	films optical properties	1014:1037	Results showed that the addition of LAB or alginate-pectin microbeads modified slightly films optical properties.
29443907	2	20	theme	bioactive	170:178	arg1	films					180:184	Novel bioactive films	164:184	Novel bioactive films	164:184	Novel bioactive films were developed from the incorporation of Lactococcus lactis into polysaccharide films.
29443907	6	21	theme	antimicrobial	822:834	arg1	effect					836:841	the antimicrobial effect	818:841	the antimicrobial effect of bioactive films against Listeria monocytogenes	818:891	Furthermore, the antimicrobial effect of bioactive films against Listeria monocytogenes was studied in synthetic medium.
29443907	10	22	theme	first	1487:1491	arg1	days					1498:1501	the first five days	1483:1501	the first five days of storage at 5 °C	1483:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	8	23	theme	beads	1151:1155	arg1	addition					1120:1127	the addition	1116:1127	the addition of bacterial cells or beads	1116:1155	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	5	24	theme	mechanical	750:759	arg1	properties					761:770	mechanical properties	750:770	mechanical properties	750:770	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	0	25	theme	Bioactive	0:8	arg1	Films					10:14	Bioactive Films	0:14	Bioactive Films	0:14	Bioactive Films Containing Alginate-Pectin Composite Microbeads with Lactococcus lactis subsp.
29443907	9	26	theme	barrier	1223:1229	arg1	properties					1231:1240	barrier properties	1223:1240	barrier properties	1223:1240	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	6	27	theme	Listeria	870:877	arg1	monocytogenes					879:891	Listeria monocytogenes	870:891	Listeria monocytogenes	870:891	Furthermore, the antimicrobial effect of bioactive films against Listeria monocytogenes was studied in synthetic medium.
29443907	5	28	theme	incorporation	618:630	arg1	impact					604:609	the impact	600:609	the impact of the incorporation of the protective culture into the biopolymer matrix	600:683	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	1	29	theme	Physicochemical	103:117	arg1	Characterization					119:134	Physicochemical Characterization	103:134	Physicochemical Characterization	103:134	lactis: Physicochemical Characterization and Antilisterial Activity.
29443907	5	30	theme	water	690:694	arg1	permeability					703:714	the water vapour permeability	686:714	the water vapour permeability	686:714	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	0	31	theme	Alginate-Pectin	27:41	arg1	Microbeads					53:62	Alginate-Pectin Composite Microbeads	27:62	Alginate-Pectin Composite Microbeads	27:62	Bioactive Films Containing Alginate-Pectin Composite Microbeads with Lactococcus lactis subsp.
29443907	7	32	theme	alginate-pectin	969:983	arg1	addition					950:957	the addition	946:957	the addition of LAB or alginate-pectin microbeads	946:994	Results showed that the addition of LAB or alginate-pectin microbeads modified slightly films optical properties.
29443907	8	33	theme	bacterial	1132:1140	arg1	cells					1142:1146	bacterial cells	1132:1146	bacterial cells	1132:1146	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	4	34	theme	free	416:419	arg1	LAB					411:413	LAB	411:413	LAB	411:413	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	4	34	theme	free	416:419	arg1	bacteria					401:408	Lactic acid bacteria	389:408	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads	389:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	6	35	theme	films	856:860	arg1	effect					836:841	the antimicrobial effect	818:841	the antimicrobial effect of bioactive films against Listeria monocytogenes	818:891	Furthermore, the antimicrobial effect of bioactive films against Listeria monocytogenes was studied in synthetic medium.
29443907	10	36	theme	starch	1372:1377	arg1	films					1379:1383	HPMC and starch films	1363:1383	HPMC and starch films containing encapsulated bioactive culture	1363:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	37	theme	complete	1436:1443	arg1	inhibition					1445:1454	a complete inhibition	1434:1454	a complete inhibition of listerial growth during the first five days of storage at 5 °C	1434:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	38	theme	logs	1543:1546	arg1	reduction					1528:1536	a reduction	1526:1536	a reduction of 5 logs	1526:1546	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	38	theme	logs	1543:1546	arg1	inhibition					1445:1454	a complete inhibition	1434:1454	a complete inhibition of listerial growth during the first five days of storage at 5 °C	1434:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	39	contain	containing	1385:1394	arg2	culture					1419:1425	encapsulated bioactive culture	1396:1425	encapsulated bioactive culture	1396:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	39	contain	containing	1385:1394	arg1	films					1379:1383	HPMC and starch films	1363:1383	HPMC and starch films containing encapsulated bioactive culture	1363:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	3	40	theme	cellulose	312:320	arg1	derivative					322:331	cellulose derivative	312:331	cellulose derivative (hydroxylpropylmethylcellulose (HPMC))	312:370	Two different biopolymers were tested: cellulose derivative (hydroxylpropylmethylcellulose (HPMC)) and corn starch.
29443907	10	41	theme	HPMC	1363:1366	arg1	films					1379:1383	HPMC and starch films	1363:1383	HPMC and starch films containing encapsulated bioactive culture	1363:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	0	42	theme	Composite	43:51	arg1	Microbeads					53:62	Alginate-Pectin Composite Microbeads	27:62	Alginate-Pectin Composite Microbeads	27:62	Bioactive Films Containing Alginate-Pectin Composite Microbeads with Lactococcus lactis subsp.
29443907	8	43	theme	HPMC	1059:1062	arg1	films					1064:1068	HPMC films	1059:1068	HPMC films	1059:1068	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	8	44	theme	cells	1142:1146	arg1	addition					1120:1127	the addition	1116:1127	the addition of bacterial cells or beads	1116:1155	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	6	45	theme	synthetic	908:916	arg1	medium					918:923	synthetic medium	908:923	synthetic medium	908:923	Furthermore, the antimicrobial effect of bioactive films against Listeria monocytogenes was studied in synthetic medium.
29443907	4	46	from	bacteria	401:408	arg1	microbeads					486:495	alginate-pectin composite hydrogel microbeads	451:495	alginate-pectin composite hydrogel microbeads	451:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	4	47	theme	acid	396:399	arg1	LAB					411:413	LAB	411:413	LAB	411:413	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	4	47	theme	acid	396:399	arg1	bacteria					401:408	Lactic acid bacteria	389:408	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads	389:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	0	48	theme	Lactococcus	69:79	arg1	lactis					81:86	Lactococcus lactis	69:86	Lactococcus lactis subsp	69:92	Bioactive Films Containing Alginate-Pectin Composite Microbeads with Lactococcus lactis subsp.
29443907	10	49	from	°C	1519:1520	arg1	storage					1506:1512	storage	1506:1512	storage at 5 °C	1506:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	49	from	°C	1519:1520	arg1	days					1498:1501	the first five days	1483:1501	the first five days of storage at 5 °C	1483:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	6	50	theme	bioactive	846:854	arg1	films					856:860	bioactive films	846:860	bioactive films	846:860	Furthermore, the antimicrobial effect of bioactive films against Listeria monocytogenes was studied in synthetic medium.
29443907	0	51	theme	lactis	81:86	arg1	subsp					88:92	Lactococcus lactis subsp	69:92	Lactococcus lactis subsp	69:92	Bioactive Films Containing Alginate-Pectin Composite Microbeads with Lactococcus lactis subsp.
29443907	4	52	theme	Lactic	389:394	arg1	LAB					411:413	LAB	411:413	LAB	411:413	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	4	52	theme	Lactic	389:394	arg1	bacteria					401:408	Lactic acid bacteria	389:408	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads	389:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	1	53	theme	Antilisterial	140:152	arg1	Activity					154:161	Antilisterial Activity	140:161	Antilisterial Activity	140:161	lactis: Physicochemical Characterization and Antilisterial Activity.
29443907	10	54	theme	growth	1469:1474	arg1	reduction					1528:1536	a reduction	1526:1536	a reduction of 5 logs	1526:1546	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	10	54	theme	growth	1469:1474	arg1	inhibition					1445:1454	a complete inhibition	1434:1454	a complete inhibition of listerial growth during the first five days of storage at 5 °C	1434:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	5	55	theme	oxygen	717:722	arg1	permeability					724:735	oxygen permeability	717:735	oxygen permeability	717:735	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	4	56	theme	hydrogel	477:484	arg1	microbeads					486:495	alginate-pectin composite hydrogel microbeads	451:495	alginate-pectin composite hydrogel microbeads	451:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	9	57	theme	interactions	1308:1319	arg1	establishment					1291:1303	the possible establishment	1278:1303	the possible establishment of interactions between alginate-pectin beads	1278:1349	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	10	58	theme	encapsulated	1396:1407	arg1	culture					1419:1425	encapsulated bioactive culture	1396:1425	encapsulated bioactive culture	1396:1425	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	9	59	theme	possible	1282:1289	arg1	establishment					1291:1303	the possible establishment	1278:1303	the possible establishment of interactions between alginate-pectin beads	1278:1349	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	7	60	theme	LAB	962:964	arg1	addition					950:957	the addition	946:957	the addition of LAB or alginate-pectin microbeads	946:994	Results showed that the addition of LAB or alginate-pectin microbeads modified slightly films optical properties.
29443907	8	61	theme	starch	1071:1076	arg1	matrix					1078:1083	starch matrix	1071:1083	starch matrix	1071:1083	In comparison with HPMC films, starch matrix proves to be more sensitive to the addition of bacterial cells or beads.
29443907	4	62	theme	composite	467:475	arg1	microbeads					486:495	alginate-pectin composite hydrogel microbeads	451:495	alginate-pectin composite hydrogel microbeads	451:495	Lactic acid bacteria (LAB) free or previously encapsulated in alginate-pectin composite hydrogel microbeads were added directly to the film forming solution and films were obtained by casting.
29443907	5	63	theme	protective	639:648	arg1	culture					650:656	the protective culture	635:656	the protective culture	635:656	In order to study the impact of the incorporation of the protective culture into the biopolymer matrix, the water vapour permeability, oxygen permeability, optical and mechanical properties of the dry films were evaluated.
29443907	10	64	theme	listerial	1459:1467	arg1	growth					1469:1474	listerial growth	1459:1474	listerial growth during the first five days of storage at 5 °C	1459:1520	HPMC and starch films containing encapsulated bioactive culture showed a complete inhibition of listerial growth during the first five days of storage at 5 °C and a reduction of 5 logs after 12 days.
29443907	9	65	theme	corn	1191:1194	arg1	films					1203:1207	corn starch films	1191:1207	corn starch films	1191:1207	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	2	66	theme	lactis	239:244	arg1	incorporation					210:222	the incorporation	206:222	the incorporation of Lactococcus lactis into polysaccharide films	206:270	Novel bioactive films were developed from the incorporation of Lactococcus lactis into polysaccharide films.
29443907	9	67	theme	mechanical	1166:1175	arg1	resistance					1177:1186	mechanical resistance	1166:1186	mechanical resistance of corn starch films	1166:1207	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	9	68	theme	starch	1196:1201	arg1	films					1203:1207	corn starch films	1191:1207	corn starch films	1191:1207	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	2	69	theme	Lactococcus	227:237	arg1	lactis					239:244	Lactococcus lactis	227:244	Lactococcus lactis	227:244	Novel bioactive films were developed from the incorporation of Lactococcus lactis into polysaccharide films.
29443907	9	70	theme	films	1203:1207	arg1	resistance					1177:1186	mechanical resistance	1166:1186	mechanical resistance of corn starch films	1166:1207	Indeed, mechanical resistance of corn starch films was lower but barrier properties were improved, certainly related to the possible establishment of interactions between alginate-pectin beads and starch.
29443907	1	71	dep	Characterization	119:134	arg1	lactis					95:100	lactis	95:100	lactis	95:100	lactis: Physicochemical Characterization and Antilisterial Activity.
29443907	3	72	dep	derivative	322:331	arg1	HPMC					365:368	HPMC	365:368	HPMC	365:368	Two different biopolymers were tested: cellulose derivative (hydroxylpropylmethylcellulose (HPMC)) and corn starch.
29443907	3	72	dep	derivative	322:331	arg1	hydroxylpropylmethylcellulose					334:362	hydroxylpropylmethylcellulose	334:362	hydroxylpropylmethylcellulose (HPMC)	334:369	Two different biopolymers were tested: cellulose derivative (hydroxylpropylmethylcellulose (HPMC)) and corn starch.
29443907	2	73	theme	Novel	164:168	arg1	films					180:184	Novel bioactive films	164:184	Novel bioactive films	164:184	Novel bioactive films were developed from the incorporation of Lactococcus lactis into polysaccharide films.
29529590	1	0	theme	composite	333:341	arg1	microspheres					343:354	composite microspheres	333:354	composite microspheres	333:354	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	1	1	theme	guided	151:156	arg1	membrane					182:189	the guided bone regeneration (GBR) membrane	147:189	the guided bone regeneration (GBR) membrane	147:189	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	1	2	theme	nHA/CS	248:253	arg1	microspheres					266:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	1	3	dep	in	301:302	arg1	situ					304:307	situ	304:307	situ	304:307	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	6	4	theme	elastic	955:961	arg1	modulus					963:969	The elastic modulus	951:969	The elastic modulus	951:969	The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively.
29529590	1	5	theme	bone	158:161	arg1	membrane					182:189	the guided bone regeneration (GBR) membrane	147:189	the guided bone regeneration (GBR) membrane	147:189	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	8	6	dep	in	1283:1284	arg1	situ					1286:1289	situ	1286:1289	situ	1286:1289	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	1	7	theme	composite	256:264	arg1	microspheres					266:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	2	8	theme	testing	550:556	arg1	machine					558:564	universal mechanical testing machine	529:564	universal mechanical testing machine	529:564	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	4	9	contain	containing	748:757	arg1	membranes					738:746	GBR membranes	734:746	GBR membranes containing in situ nHA/CS microspheres	734:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	4	9	contain	containing	748:757	arg2	microspheres					774:785	in situ nHA/CS microspheres	759:785	in situ nHA/CS microspheres	759:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	3	10	dep	in	593:594	arg1	situ					596:599	situ	596:599	situ	596:599	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	11	from	embedded	637:644	arg1	membrane					652:659	CS membrane	649:659	CS membrane	649:659	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	5	12	theme	elongation	904:913	arg1	rate					915:918	Its elongation rate	900:918	Its elongation rate at break	900:927	Its elongation rate at break reaches 5.61 ± 0.95%.
29529590	2	13	theme	mechanical	539:548	arg1	machine					558:564	universal mechanical testing machine	529:564	universal mechanical testing machine	529:564	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	8	14	theme	nHA/CS	1302:1307	arg1	microspheres					1309:1320	in situ biomimetic nHA/CS microspheres	1283:1320	in situ biomimetic nHA/CS microspheres	1283:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	4	15	theme	nHA/CS	767:772	arg1	microspheres					774:785	in situ nHA/CS microspheres	759:785	in situ nHA/CS microspheres	759:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	7	16	with	work-of-fracture	1079:1094	arg1	microspheres					1126:1137	in situ microspheres	1118:1137	in situ microspheres	1118:1137	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	4	17	dep	membranes	824:832	arg1	containing					834:843	containing	834:843	containing pure CS microspheres	834:864	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	4	17	dep	membranes	824:832	arg1	blending					870:877	blending	870:877	blending nHA/CS microspheres	870:897	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	1	18	theme	regeneration	163:174	arg1	membrane					182:189	the guided bone regeneration (GBR) membrane	147:189	the guided bone regeneration (GBR) membrane	147:189	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	2	19	theme	universal	529:537	arg1	machine					558:564	universal mechanical testing machine	529:564	universal mechanical testing machine	529:564	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	1	20	theme	CS	379:380	arg1	membrane					382:389	CS membrane	379:389	CS membrane	379:389	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	4	21	theme	nHA/CS	879:884	arg1	microspheres					886:897	nHA/CS microspheres	879:897	nHA/CS microspheres	879:897	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	8	22	contain	containing	1272:1281	arg1	membranes					1262:1270	the GBR membranes	1254:1270	the GBR membranes containing in situ biomimetic nHA/CS microspheres	1254:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	8	22	contain	containing	1272:1281	arg2	microspheres					1309:1320	in situ biomimetic nHA/CS microspheres	1283:1320	in situ biomimetic nHA/CS microspheres	1283:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	0	23	theme	chitosan	11:18	arg1	membranes					20:28	chitosan membranes	11:28	chitosan membranes	11:28	Reinforced chitosan membranes by microspheres for guided bone regeneration.
29529590	6	24	theme	membranes	995:1003	arg1	strength					975:982	strength	975:982	strength	975:982	The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively.
29529590	6	24	theme	membranes	995:1003	arg1	modulus					963:969	The elastic modulus	951:969	The elastic modulus	951:969	The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively.
29529590	3	25	theme	in	593:594	arg1	microspheres					619:630	the in situ biomimetic nHA/CS microspheres	589:630	the in situ biomimetic nHA/CS microspheres	589:630	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	25	theme	in	593:594	arg1	embedded					637:644	embedded	637:644	embedded	637:644	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	26	from	membrane	652:659	arg1	microspheres					619:630	the in situ biomimetic nHA/CS microspheres	589:630	the in situ biomimetic nHA/CS microspheres	589:630	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	26	from	membrane	652:659	arg1	embedded					637:644	embedded	637:644	embedded	637:644	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	1	27	theme	GBR	177:179	arg1	membrane					182:189	the guided bone regeneration (GBR) membrane	147:189	the guided bone regeneration (GBR) membrane	147:189	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	8	28	theme	culture	1225:1231	arg1	results					1233:1239	The cell culture results	1216:1239	The cell culture results	1216:1239	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	7	29	theme	CS	1203:1204	arg1	membrane					1206:1213	the pure CS membrane	1194:1213	the pure CS membrane	1194:1213	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	3	30	theme	CS	694:695	arg1	matrix					697:702	CS matrix	694:702	CS matrix	694:702	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	8	31	theme	biomimetic	1291:1300	arg1	microspheres					1309:1320	in situ biomimetic nHA/CS microspheres	1283:1320	in situ biomimetic nHA/CS microspheres	1283:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	2	32	theme	membranes	452:460	arg1	morphologies					396:407	morphologies	396:407	morphologies	396:407	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	2	32	theme	membranes	452:460	arg1	properties					424:433	mechanical properties	413:433	mechanical properties	413:433	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	8	33	theme	GBR	1258:1260	arg1	membranes					1262:1270	the GBR membranes	1254:1270	the GBR membranes containing in situ biomimetic nHA/CS microspheres	1254:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	1	34	theme	membrane	182:189	arg1	activity					111:118	the osteogenic activity	96:118	the osteogenic activity	96:118	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	1	34	theme	membrane	182:189	arg1	strength					135:142	mechanical strength	124:142	mechanical strength	124:142	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	2	35	theme	composite	442:450	arg1	membranes					452:460	the composite membranes	438:460	the composite membranes	438:460	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	1	36	theme	osteogenic	100:109	arg1	activity					111:118	the osteogenic activity	96:118	the osteogenic activity	96:118	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	7	37	theme	pure	1198:1201	arg1	membrane					1206:1213	the pure CS membrane	1194:1213	the pure CS membrane	1194:1213	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	1	38	theme	in	301:302	arg1	method					320:325	in situ biomimetic method	301:325	in situ biomimetic method	301:325	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	2	39	theme	electronic	497:506	arg1	SEM					520:522	SEM	520:522	SEM	520:522	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	2	39	theme	electronic	497:506	arg1	microscopy					508:517	scanning electronic microscopy	488:517	scanning electronic microscopy (SEM)	488:523	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	6	40	theme	GBR	991:993	arg1	membranes					995:1003	the GBR membranes	987:1003	the GBR membranes	987:1003	The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively.
29529590	7	41	theme	in	1118:1119	arg1	microspheres					1126:1137	in situ microspheres	1118:1137	in situ microspheres	1118:1137	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	4	42	theme	CS	850:851	arg1	microspheres					853:864	pure CS microspheres	845:864	pure CS microspheres	845:864	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	1	43	theme	bone	205:208	arg1	defect					210:215	bone defect	205:215	bone defect	205:215	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	2	44	theme	scanning	488:495	arg1	SEM					520:522	SEM	520:522	SEM	520:522	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	2	44	theme	scanning	488:495	arg1	microscopy					508:517	scanning electronic microscopy	488:517	scanning electronic microscopy (SEM)	488:523	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	1	45	theme	biomimetic	309:318	arg1	method					320:325	in situ biomimetic method	301:325	in situ biomimetic method	301:325	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	7	46	dep	in	1118:1119	arg1	situ					1121:1124	situ	1121:1124	situ	1121:1124	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	3	47	theme	biomimetic	601:610	arg1	microspheres					619:630	the in situ biomimetic nHA/CS microspheres	589:630	the in situ biomimetic nHA/CS microspheres	589:630	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	47	theme	biomimetic	601:610	arg1	embedded					637:644	embedded	637:644	embedded	637:644	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	4	48	theme	membranes	738:746	arg1	properties					720:729	The mechanical properties	705:729	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres	705:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	4	48	theme	membranes	738:746	arg1	higher					804:809	higher	804:809	higher	804:809	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	0	49	theme	guided	50:55	arg1	regeneration					62:73	guided bone regeneration	50:73	guided bone regeneration	50:73	Reinforced chitosan membranes by microspheres for guided bone regeneration.
29529590	7	50	theme	membranes	1103:1111	arg1	work-of-fracture					1079:1094	The work-of-fracture	1075:1094	The work-of-fracture of the membranes with in situ microspheres	1075:1137	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	3	51	theme	nHA/CS	612:617	arg1	microspheres					619:630	the in situ biomimetic nHA/CS microspheres	589:630	the in situ biomimetic nHA/CS microspheres	589:630	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	3	51	theme	nHA/CS	612:617	arg1	embedded					637:644	embedded	637:644	embedded	637:644	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	4	52	theme	GBR	734:736	arg1	membranes					738:746	GBR membranes	734:746	GBR membranes containing in situ nHA/CS microspheres	734:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	7	53	theme	membrane	1206:1213	arg1	times					1185:1189	3 times	1183:1189	3 times of the pure CS membrane	1183:1213	Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m2, which is about 3 times of the pure CS membrane.
29529590	3	54	theme	CS	649:650	arg1	membrane					652:659	CS membrane	649:659	CS membrane	649:659	The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix.
29529590	4	55	dep	in	759:760	arg1	situ					762:765	situ	762:765	situ	762:765	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	1	56	theme	mechanical	124:133	arg1	strength					135:142	mechanical strength	124:142	mechanical strength	124:142	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	8	57	theme	good	1330:1333	arg1	cytocompatibility					1335:1351	good cytocompatibility	1330:1351	good cytocompatibility	1330:1351	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	8	58	theme	cell	1220:1223	arg1	results					1233:1239	The cell culture results	1216:1239	The cell culture results	1216:1239	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	4	59	theme	in	759:760	arg1	microspheres					774:785	in situ nHA/CS microspheres	759:785	in situ nHA/CS microspheres	759:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	2	60	theme	mechanical	413:422	arg1	properties					424:433	mechanical properties	413:433	mechanical properties	413:433	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
29529590	4	61	theme	pure	845:848	arg1	microspheres					853:864	pure CS microspheres	845:864	pure CS microspheres	845:864	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	5	62	from	break	923:927	arg1	rate					915:918	Its elongation rate	900:918	Its elongation rate at break	900:927	Its elongation rate at break reaches 5.61 ± 0.95%.
29529590	4	63	theme	mechanical	709:718	arg1	properties					720:729	The mechanical properties	705:729	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres	705:785	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	4	63	theme	mechanical	709:718	arg1	higher					804:809	higher	804:809	higher	804:809	The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres.
29529590	8	64	theme	in	1283:1284	arg1	microspheres					1309:1320	in situ biomimetic nHA/CS microspheres	1283:1320	in situ biomimetic nHA/CS microspheres	1283:1320	The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.
29529590	1	65	theme	nano-hydroxyapatite/chitosan	218:245	arg1	microspheres					266:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres	218:277	In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane.
29529590	0	66	theme	bone	57:60	arg1	regeneration					62:73	guided bone regeneration	50:73	guided bone regeneration	50:73	Reinforced chitosan membranes by microspheres for guided bone regeneration.
29529590	2	67	dep	morphologies	396:407	arg1	The					392:394	The	392:394	The	392:394	The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine.
31574948	8	0	theme	acids	1381:1385	arg1	levels					1363:1368	the levels	1359:1368	the levels of organic acids	1359:1385	It also increased the levels of organic acids and improved the composition of intestinal microbiota.
31574948	7	1	theme	box	1291:1293	arg1	O1					1295:1296	forkhead box O1	1282:1296	forkhead box O1	1282:1296	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	9	2	theme	PHGG	1475:1478	arg1	treatment					1480:1488	PHGG treatment	1475:1488	PHGG treatment	1475:1488	These findings demonstrated that PHGG treatment could effectively alleviate the oxidative damage and dysbacteriosis.
31574948	0	3	theme	Gut	91:93	arg1	Microbiota					95:104	Gut Microbiota	91:104	Gut Microbiota in Rats	91:112	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	6	4	theme	factor	1142:1147	arg1	expressions					1100:1110	the expressions	1096:1110	the expressions of brain-derived neurotrophic factor and choline acetyltransferase	1096:1177	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	4	5	theme	mg/kg/day	633:641	arg1	concentration					611:623	a concentration	609:623	a concentration of 1500 mg/kg/day	609:641	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	5	6	from	content	957:963	arg1	brain					1001:1005	brain	1001:1005	brain	1001:1005	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	5	6	from	content	957:963	arg1	serum					991:995	rat serum	987:995	rat serum	987:995	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	2	7	theme	aging-related	264:276	arg1	injury					278:283	aging-related injury	264:283	aging-related injury which is usually resulted from oxidative stress on tissues and cells	264:352	The injection of d-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells.
31574948	3	8	theme	oxidative	497:505	arg1	damages					507:513	oxidative damages	497:513	oxidative damages	497:513	In this study, d-galactose (200 mg/kg/day) was injected into rats, and the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions, were analyzed.
31574948	2	9	from	stress	326:331	arg1	cells					348:352	cells	348:352	cells	348:352	The injection of d-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells.
31574948	2	9	from	stress	326:331	arg1	tissues					336:342	tissues	336:342	tissues	336:342	The injection of d-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells.
31574948	6	10	theme	brain-derived	1115:1127	arg1	factor					1142:1147	brain-derived neurotrophic factor	1115:1147	brain-derived neurotrophic factor	1115:1147	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	7	11	theme	tumor	1303:1307	arg1	p53					1317:1319	tumor protein p53	1303:1319	tumor protein p53	1303:1319	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	5	12	theme	malondialdehyde	968:982	arg1	content					957:963	the content	953:963	the content of malondialdehyde in rat serum and brain	953:1005	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	6	13	theme	acetyltransferase	1161:1177	arg1	expressions					1100:1110	the expressions	1096:1110	the expressions of brain-derived neurotrophic factor and choline acetyltransferase	1096:1177	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	4	14	from	concentration	611:623	arg1	treatment					596:604	PHGG treatment	591:604	PHGG treatment at a concentration of 1500 mg/kg/day	591:641	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	7	15	from	expression	1257:1266	arg1	hippocampus					1328:1338	the hippocampus	1324:1338	the hippocampus	1324:1338	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	6	16	theme	choline	1153:1159	arg1	acetyltransferase					1161:1177	choline acetyltransferase	1153:1177	choline acetyltransferase	1153:1177	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	3	17	theme	PHGG	452:455	arg1	effects					441:447	the protective effects	426:447	the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions,	426:550	In this study, d-galactose (200 mg/kg/day) was injected into rats, and the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions, were analyzed.
31574948	4	18	theme	nitric	710:715	arg1	synthase					723:730	inducible nitric oxide synthase	700:730	inducible nitric oxide synthase	700:730	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	18	theme	nitric	710:715	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	1	19	theme	considerable	165:176	arg1	attention					178:186	considerable attention	165:186	considerable attention	165:186	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	5	20	theme	rat	987:989	arg1	serum					991:995	rat serum	987:995	rat serum	987:995	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	4	21	theme	inducible	700:708	arg1	synthase					723:730	inducible nitric oxide synthase	700:730	inducible nitric oxide synthase	700:730	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	21	theme	inducible	700:708	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	0	22	theme	Hydrolyzed	10:19	arg1	Gum					26:28	Partially Hydrolyzed Guar Gum	0:28	Partially Hydrolyzed Guar Gum	0:28	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	8	23	theme	intestinal	1419:1428	arg1	microbiota					1430:1439	intestinal microbiota	1419:1439	intestinal microbiota	1419:1439	It also increased the levels of organic acids and improved the composition of intestinal microbiota.
31574948	4	24	theme	telomerase	784:793	arg1	activity					795:802	the telomerase activity	780:802	the telomerase activity	780:802	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	25	theme	acid	680:683	arg1	levels					663:668	the levels	659:668	the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products,	659:764	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	6	26	theme	neurotrophic	1129:1140	arg1	factor					1142:1147	brain-derived neurotrophic factor	1115:1147	brain-derived neurotrophic factor	1115:1147	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	4	27	theme	lactic	673:678	arg1	products					756:763	advanced glycation end products	733:763	advanced glycation end products	733:763	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	27	theme	lactic	673:678	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	27	theme	lactic	673:678	arg1	oxide					693:697	nitric oxide	686:697	nitric oxide	686:697	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	27	theme	lactic	673:678	arg1	synthase					723:730	inducible nitric oxide synthase	700:730	inducible nitric oxide synthase	700:730	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	8	28	theme	organic	1373:1379	arg1	acids					1381:1385	organic acids	1373:1385	organic acids	1373:1385	It also increased the levels of organic acids and improved the composition of intestinal microbiota.
31574948	7	29	theme	protein	1309:1315	arg1	p53					1317:1319	tumor protein p53	1303:1319	tumor protein p53	1303:1319	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	7	30	theme	forkhead	1282:1289	arg1	O1					1295:1296	forkhead box O1	1282:1296	forkhead box O1	1282:1296	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	2	31	theme	oxidative	316:324	arg1	stress					326:331	oxidative stress	316:331	oxidative stress on tissues and cells	316:352	The injection of d-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells.
31574948	2	32	theme	d-galactose	242:252	arg1	injection					229:237	The injection	225:237	The injection of d-galactose	225:252	The injection of d-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells.
31574948	1	33	theme	various	196:202	arg1	functions					214:222	its various bioactive functions	192:222	its various bioactive functions	192:222	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	0	34	theme	Guar	21:24	arg1	Gum					26:28	Partially Hydrolyzed Guar Gum	0:28	Partially Hydrolyzed Guar Gum	0:28	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	6	35	link	brain-derived	1115:1127	arg1	factor					1142:1147	brain-derived neurotrophic factor	1115:1147	brain-derived neurotrophic factor	1115:1147	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	7	36	theme	p53	1317:1319	arg1	expression					1257:1266	the expression	1253:1266	the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus	1253:1338	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	0	37	from	Microbiota	95:104	arg1	Rats					109:112	Rats	109:112	Rats	109:112	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	6	38	theme	oxidative	1012:1020	arg1	damage					1022:1027	The oxidative damage	1008:1027	The oxidative damage	1008:1027	The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased.
31574948	1	39	theme	bioactive	204:212	arg1	functions					214:222	its various bioactive functions	192:222	its various bioactive functions	192:222	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	0	40	theme	d-Galactose-Induced	41:59	arg1	Stress					71:76	d-Galactose-Induced Oxidative Stress	41:76	d-Galactose-Induced Oxidative Stress	41:76	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	4	41	theme	PHGG	591:594	arg1	treatment					596:604	PHGG treatment	591:604	PHGG treatment at a concentration of 1500 mg/kg/day	591:641	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	42	theme	end	752:754	arg1	products					756:763	advanced glycation end products	733:763	advanced glycation end products	733:763	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	42	theme	end	752:754	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	3	43	theme	protective	430:439	arg1	effects					441:447	the protective effects	426:447	the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions,	426:550	In this study, d-galactose (200 mg/kg/day) was injected into rats, and the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions, were analyzed.
31574948	4	44	theme	glycation	742:750	arg1	products					756:763	advanced glycation end products	733:763	advanced glycation end products	733:763	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	44	theme	glycation	742:750	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	8	45	theme	microbiota	1430:1439	arg1	composition					1404:1414	the composition	1400:1414	the composition of intestinal microbiota	1400:1439	It also increased the levels of organic acids and improved the composition of intestinal microbiota.
31574948	1	46	theme	hydrolyzed	125:134	arg1	gum					141:143	Partially hydrolyzed guar gum	115:143	Partially hydrolyzed guar gum (PHGG)	115:150	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	1	46	theme	hydrolyzed	125:134	arg1	PHGG					146:149	PHGG	146:149	PHGG	146:149	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	0	47	theme	Oxidative	61:69	arg1	Stress					71:76	d-Galactose-Induced Oxidative Stress	41:76	d-Galactose-Induced Oxidative Stress	41:76	Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
31574948	3	48	theme	probiotic	531:539	arg1	functions					541:549	its probiotic functions	527:549	its probiotic functions	527:549	In this study, d-galactose (200 mg/kg/day) was injected into rats, and the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions, were analyzed.
31574948	1	49	theme	guar	136:139	arg1	gum					141:143	Partially hydrolyzed guar gum	115:143	Partially hydrolyzed guar gum (PHGG)	115:150	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	1	49	theme	guar	136:139	arg1	PHGG					146:149	PHGG	146:149	PHGG	146:149	Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions.
31574948	5	50	theme	antioxidant	919:929	arg1	enzymes					931:937	antioxidant enzymes	919:937	antioxidant enzymes	919:937	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	7	51	theme	sirtuin	1271:1277	arg1	expression					1257:1266	the expression	1253:1266	the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus	1253:1338	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	7	52	theme	O1	1295:1296	arg1	expression					1257:1266	the expression	1253:1266	the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus	1253:1338	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	4	53	theme	nitric	686:691	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	53	theme	nitric	686:691	arg1	oxide					693:697	nitric oxide	686:697	nitric oxide	686:697	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	5	54	theme	enzymes	931:937	arg1	activities					905:914	the activities	901:914	the activities of antioxidant enzymes	901:937	Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain.
31574948	4	55	theme	oxide	717:721	arg1	synthase					723:730	inducible nitric oxide synthase	700:730	inducible nitric oxide synthase	700:730	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	55	theme	oxide	717:721	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	56	theme	advanced	733:740	arg1	products					756:763	advanced glycation end products	733:763	advanced glycation end products	733:763	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	4	56	theme	advanced	733:740	arg1	acid					680:683	lactic acid	673:683	lactic acid	673:683	The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively.
31574948	7	57	theme	PHGG	1208:1211	arg1	treatment					1213:1221	PHGG treatment	1208:1221	PHGG treatment	1208:1221	Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus.
31574948	9	58	theme	oxidative	1522:1530	arg1	damage					1532:1537	the oxidative damage	1518:1537	the oxidative damage	1518:1537	These findings demonstrated that PHGG treatment could effectively alleviate the oxidative damage and dysbacteriosis.
31789494	6	0	theme	human	1003:1007	arg1	cells					1030:1034	human adipose-derived stem cells	1003:1034	human adipose-derived stem cells	1003:1034	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	1	1	theme	interfaces	185:194	arg1	profile					151:157	The innate graded structural and compositional profile	104:157	The innate graded structural and compositional profile of musculoskeletal tissue interfaces	104:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	1	2	theme	degeneration	271:282	arg1	context					248:254	the context	244:254	the context of disease and degeneration	244:282	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	7	3	theme	complex	1479:1485	arg1	intersections					1487:1499	the graded, composite, and complex intersections	1452:1499	the graded, composite, and complex intersections of the connective tissues	1452:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	6	4	theme	phosphatase	1222:1232	arg1	activity					1188:1195	the activity	1184:1195	the activity of the secreted alkaline phosphatase	1184:1232	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	4	theme	phosphatase	1222:1232	arg1	expression					1242:1251	the expression	1238:1251	the expression of osteopontin	1238:1266	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	4	5	theme	hydroxyapatite	626:639	arg1	particles					641:649	hydroxyapatite particles	626:649	hydroxyapatite particles	626:649	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	6	6	theme	commitment	987:996	arg1	evaluation					958:967	The evaluation	954:967	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface	954:1070	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	4	7	theme	cellulose	654:662	arg1	CNC					678:680	CNC	678:680	CNC	678:680	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	4	7	theme	cellulose	654:662	arg1	nanocrystals					664:675	cellulose nanocrystals	654:675	cellulose nanocrystals (CNC)	654:681	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	5	8	theme	Stiffer	798:804	arg1	hydrogels					806:814	Stiffer hydrogels	798:814	Stiffer hydrogels	798:814	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	6	9	theme	biological	976:985	arg1	commitment					987:996	the biological commitment	972:996	the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface	972:1070	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	4	10	dep	bone	761:764	arg1	tissue					776:781	tissue	776:781	tissue	776:781	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	3	11	theme	distinct	537:544	arg1	composition					546:556	distinct composition	537:556	distinct composition	537:556	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	6	12	link	adipose-derived	1009:1023	arg1	cells					1030:1034	human adipose-derived stem cells	1003:1034	human adipose-derived stem cells	1003:1034	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	3	13	theme	multiphasic	488:498	arg1	system					509:514	a gelatin-based multiphasic hydrogel system	472:514	a gelatin-based multiphasic hydrogel system	472:514	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	6	14	theme	aligned	1084:1090	arg1	growth					1097:1102	an aligned cell growth	1081:1102	an aligned cell growth	1081:1102	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	3	15	theme	single	598:603	arg1	unit					605:608	a single unit	596:608	a single unit	596:608	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	3	16	theme	hydrogel	500:507	arg1	system					509:514	a gelatin-based multiphasic hydrogel system	472:514	a gelatin-based multiphasic hydrogel system	472:514	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	2	17	located	found	373:377	arg2	complexity					362:371	the transitional complexity	345:371	the transitional complexity found in those junctions	345:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	2	17	located	found	373:377	arg1	junctions					388:396	those junctions	382:396	those junctions	382:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	7	18	theme	gelatin-transglutaminase	1368:1391	arg1	enzyme					1393:1398	gelatin-transglutaminase enzyme	1368:1398	gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues	1368:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	2	19	theme	transitional	349:360	arg1	complexity					362:371	the transitional complexity	345:371	the transitional complexity found in those junctions	345:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	7	20	theme	tandem	1400:1405	arg1	enzyme					1393:1398	gelatin-transglutaminase enzyme	1368:1398	gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues	1368:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	2	21	theme	regenerative	428:439	arg1	medicine					441:448	regenerative medicine	428:448	regenerative medicine	428:448	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	0	22	theme	Biphasic	0:7	arg1	Hydrogels					9:17	Biphasic Hydrogels	0:17	Biphasic Hydrogels	0:17	Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering.
31789494	5	23	theme	structure	933:941	arg1	formation					943:951	anisotropic structure formation	921:951	anisotropic structure formation	921:951	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	1	24	theme	innate	108:113	arg1	profile					151:157	The innate graded structural and compositional profile	104:157	The innate graded structural and compositional profile of musculoskeletal tissue interfaces	104:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	3	25	theme	gelatin-based	474:486	arg1	system					509:514	a gelatin-based multiphasic hydrogel system	472:514	a gelatin-based multiphasic hydrogel system	472:514	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	0	26	theme	Mineralized	31:41	arg1	Features					59:66	Mineralized and Anisotropic Features	31:66	Mineralized and Anisotropic Features	31:66	Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering.
31789494	5	27	theme	magnetic	883:890	arg1	alignment					892:900	magnetic alignment	883:900	magnetic alignment of CNC	883:907	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	1	28	theme	graded	115:120	arg1	profile					151:157	The innate graded structural and compositional profile	104:157	The innate graded structural and compositional profile of musculoskeletal tissue interfaces	104:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	1	29	theme	fibrotic	225:232	arg1	tissue					234:239	fibrotic tissue	225:239	fibrotic tissue	225:239	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	6	30	theme	secreted	1204:1211	arg1	phosphatase					1222:1232	the secreted alkaline phosphatase	1200:1232	the secreted alkaline phosphatase	1200:1232	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	31	theme	mineralized	1288:1298	arg1	phase					1300:1304	the mineralized phase	1284:1304	the mineralized phase	1284:1304	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	1	32	theme	structural	122:131	arg1	profile					151:157	The innate graded structural and compositional profile	104:157	The innate graded structural and compositional profile of musculoskeletal tissue interfaces	104:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	0	33	theme	Anisotropic	47:57	arg1	Features					59:66	Mineralized and Anisotropic Features	31:66	Mineralized and Anisotropic Features	31:66	Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering.
31789494	6	34	theme	tendon-to-bone	1047:1060	arg1	interface					1062:1070	the tendon-to-bone interface	1043:1070	the tendon-to-bone interface	1043:1070	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	35	theme	tenascin	1143:1150	arg1	growth					1097:1102	an aligned cell growth	1081:1102	an aligned cell growth	1081:1102	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	35	theme	tenascin	1143:1150	arg1	synthesis					1115:1123	higher synthesis	1108:1123	higher synthesis	1108:1123	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	35	theme	tenascin	1143:1150	arg1	deposition					1129:1138	deposition	1129:1138	deposition	1129:1138	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	4	36	theme	gelatin	736:742	arg1	network					744:750	an enzymatically cross-linked gelatin network	706:750	an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively	706:795	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	6	37	theme	osteopontin	1256:1266	arg1	activity					1188:1195	the activity	1184:1195	the activity of the secreted alkaline phosphatase	1184:1232	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	37	theme	osteopontin	1256:1266	arg1	expression					1242:1251	the expression	1238:1251	the expression of osteopontin	1238:1266	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	4	38	theme	cross-linked	723:734	arg1	network					744:750	an enzymatically cross-linked gelatin network	706:750	an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively	706:795	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	3	39	with	sections	523:530	arg1	composition					546:556	distinct composition	537:556	distinct composition	537:556	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	3	39	with	sections	523:530	arg1	microstructure					562:575	microstructure	562:575	microstructure	562:575	Herein, we developed a gelatin-based multiphasic hydrogel system, where sections with distinct composition and microstructure were integrated in a single unit.
31789494	7	40	theme	graded	1456:1461	arg1	intersections					1487:1499	the graded, composite, and complex intersections	1452:1499	the graded, composite, and complex intersections of the connective tissues	1452:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	7	41	theme	connective	1508:1517	arg1	tissues					1519:1525	the connective tissues	1504:1525	the connective tissues	1504:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	5	42	theme	anisotropic	921:931	arg1	formation					943:951	anisotropic structure formation	921:951	anisotropic structure formation	921:951	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	5	43	theme	particles	868:876	arg1	incorporation					839:851	the incorporation	835:851	the incorporation of mineralized particles	835:876	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	7	44	theme	potential	1335:1343	arg1	versatility					1345:1355	the potential versatility	1331:1355	the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues	1331:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	4	45	link	cross-linked	723:734	arg1	network					744:750	an enzymatically cross-linked gelatin network	706:750	an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively	706:795	In each phase, hydroxyapatite particles or cellulose nanocrystals (CNC) were incorporated into an enzymatically cross-linked gelatin network to mimic bone or tendon tissue, respectively.
31789494	6	46	theme	anisotropic	1159:1169	arg1	phase					1171:1175	the anisotropic phase	1155:1175	the anisotropic phase	1155:1175	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	1	47	theme	tissue	178:183	arg1	interfaces					185:194	musculoskeletal tissue interfaces	162:194	musculoskeletal tissue interfaces	162:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	1	48	theme	compositional	137:149	arg1	profile					151:157	The innate graded structural and compositional profile	104:157	The innate graded structural and compositional profile of musculoskeletal tissue interfaces	104:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	6	49	theme	stem	1025:1028	arg1	cells					1030:1034	human adipose-derived stem cells	1003:1034	human adipose-derived stem cells	1003:1034	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	2	50	theme	engineering	292:302	arg1	strategies					304:313	Tissue engineering strategies	285:313	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions	285:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	6	51	theme	alkaline	1213:1220	arg1	phosphatase					1222:1232	the secreted alkaline phosphatase	1200:1232	the secreted alkaline phosphatase	1200:1232	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	7	52	theme	tissues	1519:1525	arg1	intersections					1487:1499	the graded, composite, and complex intersections	1452:1499	the graded, composite, and complex intersections of the connective tissues	1452:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	6	53	theme	cell	1092:1095	arg1	growth					1097:1102	an aligned cell growth	1081:1102	an aligned cell growth	1081:1102	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	6	54	with	commitment	987:996	arg1	cells					1030:1034	human adipose-derived stem cells	1003:1034	human adipose-derived stem cells	1003:1034	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	2	55	theme	Tissue	285:290	arg1	strategies					304:313	Tissue engineering strategies	285:313	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions	285:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	2	56	theme	complexity	362:371	arg1	restoration					330:340	the restoration	326:340	the restoration of the transitional complexity found in those junctions	326:396	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	0	57	theme	Tissue	84:89	arg1	Engineering					91:101	Interfacial Tissue Engineering	72:101	Interfacial Tissue Engineering	72:101	Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering.
31789494	7	58	theme	composite	1464:1472	arg1	intersections					1487:1499	the graded, composite, and complex intersections	1452:1499	the graded, composite, and complex intersections of the connective tissues	1452:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	5	59	theme	CNC	905:907	arg1	alignment					892:900	magnetic alignment	883:900	magnetic alignment of CNC	883:907	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	1	60	theme	musculoskeletal	162:176	arg1	interfaces					185:194	musculoskeletal tissue interfaces	162:194	musculoskeletal tissue interfaces	162:194	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	7	61	theme	strategies	1430:1439	arg1	development					1415:1425	the development	1411:1425	the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues	1411:1525	These results highlight the potential versatility offered by gelatin-transglutaminase enzyme tandem for the development of strategies that mimic the graded, composite, and complex intersections of the connective tissues.
31789494	1	62	theme	disease	259:265	arg1	context					248:254	the context	244:254	the context of disease and degeneration	244:282	The innate graded structural and compositional profile of musculoskeletal tissue interfaces is disrupted and replaced by fibrotic tissue in the context of disease and degeneration.
31789494	0	63	theme	Interfacial	72:82	arg1	Engineering					91:101	Interfacial Tissue Engineering	72:101	Interfacial Tissue Engineering	72:101	Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering.
31789494	6	64	theme	adipose-derived	1009:1023	arg1	cells					1030:1034	human adipose-derived stem cells	1003:1034	human adipose-derived stem cells	1003:1034	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
31789494	5	65	theme	mineralized	856:866	arg1	particles					868:876	mineralized particles	856:876	mineralized particles	856:876	Stiffer hydrogels were produced with the incorporation of mineralized particles, and magnetic alignment of CNC resulted in anisotropic structure formation.
31789494	2	66	theme	special	406:412	arg1	relevance					414:422	special relevance	406:422	special relevance for regenerative medicine	406:448	Tissue engineering strategies focused on the restoration of the transitional complexity found in those junctions present special relevance for regenerative medicine.
31789494	6	67	theme	higher	1108:1113	arg1	synthesis					1115:1123	higher synthesis	1108:1123	higher synthesis	1108:1123	The evaluation of the biological commitment with human adipose-derived stem cells toward the tendon-to-bone interface revealed an aligned cell growth and higher synthesis and deposition of tenascin in the anisotropic phase, while the activity of the secreted alkaline phosphatase and the expression of osteopontin were induced in the mineralized phase.
29606428	1	0	from	samples	199:205	arg1	Determination					132:144	Determination	132:144	Determination of the oligosaccharide composition in different wort samples	132:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	3	1	theme	first	539:543	arg1	ferments					545:552	The former first ferments	528:552	The former first ferments	528:552	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	1	2	theme	different	184:192	arg1	samples					199:205	different wort samples	184:205	different wort samples	184:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	1	3	theme	different	276:284	arg1	types					292:296	different yeast types	276:296	different yeast types	276:296	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	0	4	from	Analysis	0:7	arg1	samples					52:58	wort samples	47:58	wort samples	47:58	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	0	5	with	electrophoresis	73:87	arg1	laser					94:98	laser	94:98	laser	94:98	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	5	6	theme	oligosaccharide	920:934	arg1	signatures					936:945	unique oligosaccharide signatures	913:945	unique oligosaccharide signatures	913:945	The CE-LIF traces of the wort samples represented unique oligosaccharide signatures.
29606428	1	7	theme	yeast	286:290	arg1	types					292:296	different yeast types	276:296	different yeast types	276:296	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	0	8	from	samples	52:58	arg1	Analysis					0:7	Analysis	0:7	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser	0:98	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	3	9	theme	higher	584:589	arg1	oligomers					597:605	the higher sugar oligomers	580:605	the higher sugar oligomers	580:605	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	5	10	theme	CE-LIF	867:872	arg1	traces					874:879	The CE-LIF traces	863:879	The CE-LIF traces of the wort samples	863:899	The CE-LIF traces of the wort samples represented unique oligosaccharide signatures.
29606428	1	11	from	composition	169:179	arg1	samples					199:205	different wort samples	184:205	different wort samples	184:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	1	12	theme	wort	194:197	arg1	samples					199:205	different wort samples	184:205	different wort samples	184:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	0	13	theme	fluorescence	108:119	arg1	detection					121:129	fluorescence detection	108:129	fluorescence detection	108:129	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	3	14	theme	low	729:731	arg1	percentages					733:743	only very low percentages	719:743	only very low percentages of the oligosaccharides	719:767	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	4	15	theme	Saccharomycodes	795:809	arg1	ludwigii					811:818	Saccharomycodes ludwigii	795:818	Saccharomycodes ludwigii	795:818	Therefore, breweries use Saccharomycodes ludwigii to produce beers with low alcohol content.
29606428	5	16	theme	wort	888:891	arg1	samples					893:899	the wort samples	884:899	the wort samples	884:899	The CE-LIF traces of the wort samples represented unique oligosaccharide signatures.
29606428	0	17	from	composition	32:42	arg1	samples					52:58	wort samples	47:58	wort samples	47:58	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	5	18	theme	samples	893:899	arg1	traces					874:879	The CE-LIF traces	863:879	The CE-LIF traces of the wort samples	863:899	The CE-LIF traces of the wort samples represented unique oligosaccharide signatures.
29606428	2	19	theme	yeasts	464:469	arg1	types					455:459	two different types	441:459	two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii	441:525	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	0	20	theme	oligosaccharide	16:30	arg1	composition					32:42	the oligosaccharide composition	12:42	the oligosaccharide composition in wort samples	12:58	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	1	21	from	Determination	132:144	arg1	samples					199:205	different wort samples	184:205	different wort samples	184:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	3	22	theme	sugar	591:595	arg1	oligomers					597:605	the higher sugar oligomers	580:605	the higher sugar oligomers	580:605	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	2	23	theme	different	445:453	arg1	types					455:459	two different types	441:459	two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii	441:525	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	2	24	theme	types	455:459	arg1	effect					431:436	the effect	427:436	the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii	427:525	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	3	25	theme	oligosaccharides	752:767	arg1	percentages					733:743	only very low percentages	719:743	only very low percentages of the oligosaccharides	719:767	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	3	26	dep	such	608:611	arg1	as					613:614	as	613:614	as	613:614	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	1	27	theme	oligosaccharide	153:167	arg1	composition					169:179	the oligosaccharide composition	149:179	the oligosaccharide composition in different wort samples	149:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	3	28	theme	former	532:537	arg1	ferments					545:552	The former first ferments	528:552	The former first ferments	528:552	The former first ferments the monosaccharides, then the higher sugar oligomers, such as maltose and maltotriose, to ethanol, while the latter fully ferments the monosaccharides, but ferments only very low percentages of the oligosaccharides.
29606428	4	29	theme	alcohol	846:852	arg1	content					854:860	low alcohol content	842:860	low alcohol content	842:860	Therefore, breweries use Saccharomycodes ludwigii to produce beers with low alcohol content.
29606428	0	30	theme	composition	32:42	arg1	Analysis					0:7	Analysis	0:7	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser	0:98	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	4	31	with	beers	831:835	arg1	content					854:860	low alcohol content	842:860	low alcohol content	842:860	Therefore, breweries use Saccharomycodes ludwigii to produce beers with low alcohol content.
29606428	4	32	theme	low	842:844	arg1	content					854:860	low alcohol content	842:860	low alcohol content	842:860	Therefore, breweries use Saccharomycodes ludwigii to produce beers with low alcohol content.
29606428	5	33	theme	unique	913:918	arg1	signatures					936:945	unique oligosaccharide signatures	913:945	unique oligosaccharide signatures	913:945	The CE-LIF traces of the wort samples represented unique oligosaccharide signatures.
29606428	2	34	theme	sugars	365:370	arg1	concentration					316:328	the concentration	312:328	the concentration of fermentable and non-fermentable sugars	312:370	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	0	35	theme	wort	47:50	arg1	samples					52:58	wort samples	47:58	wort samples	47:58	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	2	36	theme	non-fermentable	349:363	arg1	sugars					365:370	fermentable and non-fermentable sugars	333:370	fermentable and non-fermentable sugars	333:370	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	2	37	theme	fermentable	333:343	arg1	sugars					365:370	fermentable and non-fermentable sugars	333:370	fermentable and non-fermentable sugars	333:370	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	0	38	theme	capillary	63:71	arg1	electrophoresis					73:87	capillary electrophoresis	63:87	capillary electrophoresis with laser	63:98	Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection.
29606428	1	39	with	process	263:269	arg1	types					292:296	different yeast types	276:296	different yeast types	276:296	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	2	40	theme	capillary	390:398	arg1	electrophoresis					400:414	capillary electrophoresis	390:414	capillary electrophoresis	390:414	In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii.
29606428	1	41	theme	brewing	255:261	arg1	process					263:269	the brewing process	251:269	the brewing process with different yeast types	251:296	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
29606428	1	42	theme	composition	169:179	arg1	Determination					132:144	Determination	132:144	Determination of the oligosaccharide composition in different wort samples	132:205	Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types.
31775217	6	0	theme	Differential	938:949	arg1	DSC					973:975	DSC	973:975	DSC	973:975	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	6	0	theme	Differential	938:949	arg1	calorimetry					960:970	Differential scanning calorimetry	938:970	Differential scanning calorimetry (DSC)	938:976	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	10	1	theme	physical	1644:1651	arg1	properties					1653:1662	the physical properties	1640:1662	the physical properties of the film	1640:1674	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	4	2	theme	tensile	742:748	arg1	strength					750:757	the highest tensile strength	730:757	the highest tensile strength	730:757	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	7	3	theme	RPP	1254:1256	arg1	addition					1235:1242	the addition	1231:1242	the addition of PS and RPP	1231:1256	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	4	4	theme	0.5	717:719	arg1	%					720:720	%	720:720	%	720:720	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	7	5	theme	PS	1247:1248	arg1	addition					1235:1242	the addition	1231:1242	the addition of PS and RPP	1231:1256	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	10	6	theme	film	1671:1674	arg1	properties					1653:1662	the physical properties	1640:1662	the physical properties of the film	1640:1674	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	11	7	theme	food	1772:1775	arg1	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	7	theme	food	1772:1775	arg1	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	3	8	theme	water	636:640	arg1	permeability					648:659	water vapor permeability	636:659	water vapor permeability (WVP)	636:665	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	8	theme	water	636:640	arg1	WVP					662:664	WVP	662:664	WVP	662:664	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	9	theme	film	486:489	arg1	thickness					491:499	film thickness	486:499	film thickness	486:499	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	1	10	theme	Pachyrhizus	190:200	arg1	PS					210:211	PS	210:211	PS	210:211	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	10	theme	Pachyrhizus	190:200	arg1	starch					202:207	Pachyrhizus starch	190:207	Pachyrhizus starch (PS)	190:212	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	11	11	theme	packaging	1777:1785	arg1	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	11	theme	packaging	1777:1785	arg1	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	0	12	from	Collagen	57:64	arg1	Properties					9:18	Physical Properties	0:18	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.	0:117	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	0	12	from	Collagen	57:64	arg1	Films					33:37	Composite Films	23:37	Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics	23:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	6	13	theme	films	1061:1065	arg1	stability					1044:1052	the thermal stability	1032:1052	the thermal stability of TSC films	1032:1065	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	3	14	theme	enhanced	511:518	arg1	trend					520:524	an enhanced trend	508:524	an enhanced trend	508:524	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	1	15	theme	Different	119:127	arg1	films					139:143	Different composite films	119:143	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP)	119:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	7	16	theme	films	1190:1194	arg1	structure					1173:1181	the amorphous structure	1159:1181	the amorphous structure of the films	1159:1194	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	3	17	theme	RPP	549:551	arg1	contents					553:560	PS and RPP contents	542:560	PS and RPP contents in TSC films	542:573	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	8	18	theme	spectroscopy	1298:1309	arg1	analysis					1318:1325	spectroscopy (FTIR) analysis	1298:1325	spectroscopy (FTIR) analysis	1298:1325	As shown by fourier transform infrared spectroscopy (FTIR) analysis, PS and RPP can strongly interact with TSC, resulting in a modification of its structure.
31775217	6	19	theme	thermal	1036:1042	arg1	stability					1044:1052	the thermal stability	1032:1052	the thermal stability of TSC films	1032:1065	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	10	20	theme	%	1586:1586	arg1	PS					1588:1589	10% PS	1584:1589	10% PS	1584:1589	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	9	21	theme	good	1485:1488	arg1	compatibility					1490:1502	a good compatibility	1483:1502	a good compatibility between TSC, PS, and RPP	1483:1527	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	4	22	with	film	696:699	arg1	RPP					722:724	0.5% RPP	717:724	0.5% RPP	717:724	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	4	22	with	film	696:699	arg1	PS					710:711	10% PS	706:711	10% PS	706:711	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	0	23	theme	Pachyrhizus	71:81	arg1	Starch					83:88	Pachyrhizus Starch	71:88	Pachyrhizus Starch	71:88	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	1	24	theme	films	299:303	arg1	properties					279:288	the physical properties	266:288	the physical properties of these films	266:303	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	25	theme	skin	165:168	arg1	TSC					180:182	TSC	180:182	TSC	180:182	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	25	theme	skin	165:168	arg1	collagen					170:177	tilapia skin collagen	157:177	tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP)	157:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	4	26	contain	had	726:728	arg2	strength					750:757	the highest tensile strength	730:757	the highest tensile strength	730:757	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	4	26	contain	had	726:728	arg1	film					696:699	TSC film	692:699	TSC film with 10% PS and 0.5% RPP	692:724	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	2	27	theme	physical	440:447	arg1	properties					449:458	the physical properties	436:458	the physical properties of TSC films	436:471	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	2	28	theme	RPP	344:346	arg1	effects					326:332	The effects	322:332	The effects of PS and RPP on TSC films	322:359	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	2	29	theme	PS	337:338	arg1	effects					326:332	The effects	322:332	The effects of PS and RPP on TSC films	322:359	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	0	30	theme	Physical	0:7	arg1	Properties					9:18	Physical Properties	0:18	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.	0:117	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	4	31	theme	10	706:707	arg1	%					708:708	%	708:708	%	708:708	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	2	32	theme	TSC	351:353	arg1	films					355:359	TSC films	351:359	TSC films	351:359	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	0	33	theme	Films	33:37	arg1	Properties					9:18	Physical Properties	0:18	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.	0:117	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	8	34	dep	fourier	1271:1277	arg1	transform					1279:1287	transform	1279:1287	transform infrared spectroscopy (FTIR) analysis	1279:1325	As shown by fourier transform infrared spectroscopy (FTIR) analysis, PS and RPP can strongly interact with TSC, resulting in a modification of its structure.
31775217	10	35	theme	%	1598:1598	arg1	RPP					1600:1602	0.5% RPP	1595:1602	0.5% RPP	1595:1602	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	0	36	theme	Tilapia	44:50	arg1	Collagen					57:64	Tilapia Skin Collagen	44:64	Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics	44:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	1	37	theme	physical	270:277	arg1	properties					279:288	the physical properties	266:288	the physical properties of these films	266:303	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	3	38	theme	PS	542:543	arg1	contents					553:560	PS and RPP contents	542:560	PS and RPP contents in TSC films	542:573	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	9	39	theme	Scanning	1417:1424	arg1	SEM					1447:1449	SEM	1447:1449	SEM	1447:1449	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	9	39	theme	Scanning	1417:1424	arg1	microscope					1435:1444	Scanning electron microscope	1417:1444	Scanning electron microscope (SEM) analysis	1417:1459	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	10	40	theme	effective	1611:1619	arg1	method					1621:1626	an effective method	1608:1626	an effective method for improve the physical properties of the film	1608:1674	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	10	40	theme	effective	1611:1619	arg1	film					1561:1564	TSC film	1557:1564	TSC film incorporated with 10% PS and 0.5% RPP	1557:1602	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	9	41	theme	microscope	1435:1444	arg1	analysis					1452:1459	Scanning electron microscope (SEM) analysis	1417:1459	Scanning electron microscope (SEM) analysis	1417:1459	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	6	42	theme	scanning	951:958	arg1	DSC					973:975	DSC	973:975	DSC	973:975	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	6	42	theme	scanning	951:958	arg1	calorimetry					960:970	Differential scanning calorimetry	938:970	Differential scanning calorimetry (DSC)	938:976	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	3	43	from	solubility	584:593	arg1	permeability					648:659	water vapor permeability	636:659	water vapor permeability (WVP)	636:665	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	43	from	solubility	584:593	arg1	elongation-at-break					605:623	elongation-at-break	605:623	elongation-at-break (EAB)	605:629	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	43	from	solubility	584:593	arg1	water					598:602	water	598:602	water	598:602	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	43	from	solubility	584:593	arg1	EAB					626:628	EAB	626:628	EAB	626:628	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	43	from	solubility	584:593	arg1	WVP					662:664	WVP	662:664	WVP	662:664	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	1	44	theme	peel	226:229	arg1	phenolics					231:239	rambutan peel phenolics	217:239	rambutan peel phenolics (RPP)	217:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	44	theme	peel	226:229	arg1	RPP					242:244	RPP	242:244	RPP	242:244	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	3	45	from	contents	553:560	arg1	films					569:573	TSC films	565:573	TSC films	565:573	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	11	46	theme	active	1765:1770	arg1	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	46	theme	active	1765:1770	arg1	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	4	47	theme	highest	734:740	arg1	strength					750:757	the highest tensile strength	730:757	the highest tensile strength	730:757	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	6	48	theme	RPP	1019:1021	arg1	addition					1000:1007	the addition	996:1007	the addition of PS and RPP	996:1021	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	0	49	with	Collagen	57:64	arg1	Starch					83:88	Pachyrhizus Starch	71:88	Pachyrhizus Starch	71:88	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	0	49	with	Collagen	57:64	arg1	Phenolics					108:116	Phenolics	108:116	Phenolics	108:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	11	50	theme	biomedical	1728:1737	arg1	applications					1739:1750	biomedical applications	1728:1750	biomedical applications	1728:1750	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	6	51	theme	PS	1012:1013	arg1	addition					1000:1007	the addition	996:1007	the addition of PS and RPP	996:1021	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	10	52	theme	TSC	1557:1559	arg1	method					1621:1626	an effective method	1608:1626	an effective method for improve the physical properties of the film	1608:1674	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	10	52	theme	TSC	1557:1559	arg1	film					1561:1564	TSC film	1557:1564	TSC film incorporated with 10% PS and 0.5% RPP	1557:1602	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	4	53	theme	%	720:720	arg1	RPP					722:724	0.5% RPP	717:724	0.5% RPP	717:724	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	2	54	from	effects	326:332	arg1	films					355:359	TSC films	351:359	TSC films	351:359	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	4	55	theme	tensile	768:774	arg1	strength					776:783	the tensile strength	764:783	the tensile strength	764:783	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	3	56	dep	showed	501:506	arg1	whereas					576:582	whereas	576:582	whereas	576:582	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	57	theme	vapor	642:646	arg1	permeability					648:659	water vapor permeability	636:659	water vapor permeability (WVP)	636:665	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	3	57	theme	vapor	642:646	arg1	WVP					662:664	WVP	662:664	WVP	662:664	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	5	58	theme	PS	926:927	arg1	incorporation					909:921	the incorporation	905:921	the incorporation of PS and RPP	905:935	The light transmittances of the films could decrease with the incorporation of PS and RPP.
31775217	10	59	theme	10	1584:1585	arg1	%					1586:1586	%	1586:1586	%	1586:1586	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	2	60	theme	TSC	463:465	arg1	films					467:471	TSC films	463:471	TSC films	463:471	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	3	61	theme	declining	674:682	arg1	trends					684:689	declining trends	674:689	declining trends	674:689	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	5	62	theme	RPP	933:935	arg1	incorporation					909:921	the incorporation	905:921	the incorporation of PS and RPP	905:935	The light transmittances of the films could decrease with the incorporation of PS and RPP.
31775217	11	63	theme	composite	1688:1696	arg1	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	63	theme	composite	1688:1696	arg1	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	6	64	theme	TSC	1057:1059	arg1	films					1061:1065	TSC films	1057:1065	TSC films	1057:1065	Differential scanning calorimetry (DSC) demonstrated that the addition of PS and RPP improved the thermal stability of TSC films.
31775217	10	65	theme	0.5	1595:1597	arg1	%					1598:1598	%	1598:1598	%	1598:1598	The results indicated that TSC film incorporated with 10% PS and 0.5% RPP was an effective method for improve the physical properties of the film.
31775217	1	66	theme	composite	129:137	arg1	films					139:143	Different composite films	119:143	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP)	119:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	4	67	theme	PS	825:826	arg1	content					814:820	the content	810:820	the content of PS and RPP	810:834	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	1	68	theme	tilapia	157:163	arg1	TSC					180:182	TSC	180:182	TSC	180:182	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	68	theme	tilapia	157:163	arg1	collagen					170:177	tilapia skin collagen	157:177	tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP)	157:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	4	69	theme	RPP	832:834	arg1	content					814:820	the content	810:820	the content of PS and RPP	810:834	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	0	70	from	Properties	9:18	arg1	Collagen					57:64	Tilapia Skin Collagen	44:64	Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics	44:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	7	71	theme	amorphous	1163:1171	arg1	structure					1173:1181	the amorphous structure	1159:1181	the amorphous structure of the films	1159:1194	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	2	72	theme	films	467:471	arg1	properties					449:458	the physical properties	436:458	the physical properties of TSC films	436:471	The effects of PS and RPP on TSC films were investigated, and our results indicated that PS and RPP could improve the physical properties of TSC films.
31775217	0	73	theme	Composite	23:31	arg1	Films					33:37	Composite Films	23:37	Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics	23:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	5	74	theme	light	851:855	arg1	transmittances					857:870	The light transmittances	847:870	The light transmittances of the films	847:883	The light transmittances of the films could decrease with the incorporation of PS and RPP.
31775217	11	75	used	used	1711:1714	arg2	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	75	used	used	1711:1714	arg2	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	7	76	theme	films	1139:1143	arg1	crystallinity					1118:1130	the crystallinity	1114:1130	the crystallinity of the films	1114:1143	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	3	77	theme	TSC	565:567	arg1	films					569:573	TSC films	565:573	TSC films	565:573	Opacity and film thickness showed an enhanced trend with increasing PS and RPP contents in TSC films, whereas solubility in water, elongation-at-break (EAB), and water vapor permeability (WVP) showed declining trends.
31775217	11	78	theme	TSC-PS-RPP	1677:1686	arg1	films					1698:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films	1677:1702	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	11	78	theme	TSC-PS-RPP	1677:1686	arg1	materials					1787:1795	active food packaging materials	1765:1795	active food packaging materials	1765:1795	TSC-PS-RPP composite films can be used not only in biomedical applications, but also as active food packaging materials.
31775217	8	79	dep	transform	1279:1287	arg1	infrared					1289:1296	infrared	1289:1296	transform infrared spectroscopy (FTIR) analysis	1279:1325	As shown by fourier transform infrared spectroscopy (FTIR) analysis, PS and RPP can strongly interact with TSC, resulting in a modification of its structure.
31775217	0	80	theme	Skin	52:55	arg1	Collagen					57:64	Tilapia Skin Collagen	44:64	Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics	44:116	Physical Properties of Composite Films from Tilapia Skin Collagen with Pachyrhizus Starch and Rambutan Peel Phenolics.
31775217	5	81	theme	films	879:883	arg1	transmittances					857:870	The light transmittances	847:870	The light transmittances of the films	847:883	The light transmittances of the films could decrease with the incorporation of PS and RPP.
31775217	4	82	theme	%	708:708	arg1	PS					710:711	10% PS	706:711	10% PS	706:711	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	1	83	with	collagen	170:177	arg1	phenolics					231:239	rambutan peel phenolics	217:239	rambutan peel phenolics (RPP)	217:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	83	with	collagen	170:177	arg1	PS					210:211	PS	210:211	PS	210:211	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	83	with	collagen	170:177	arg1	starch					202:207	Pachyrhizus starch	190:207	Pachyrhizus starch (PS)	190:212	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	83	with	collagen	170:177	arg1	RPP					242:244	RPP	242:244	RPP	242:244	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	9	84	theme	electron	1426:1433	arg1	SEM					1447:1449	SEM	1447:1449	SEM	1447:1449	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	9	84	theme	electron	1426:1433	arg1	microscope					1435:1444	Scanning electron microscope	1417:1444	Scanning electron microscope (SEM) analysis	1417:1459	Scanning electron microscope (SEM) analysis showed that there was a good compatibility between TSC, PS, and RPP.
31775217	7	85	theme	X-ray	1081:1085	arg1	diffraction					1087:1097	X-ray diffraction	1081:1097	X-ray diffraction	1081:1097	In addition, X-ray diffraction indicated that the crystallinity of the films decreased and the amorphous structure of the films tended to become more complex with the addition of PS and RPP.
31775217	8	86	theme	structure	1406:1414	arg1	modification					1386:1397	a modification	1384:1397	a modification of its structure	1384:1414	As shown by fourier transform infrared spectroscopy (FTIR) analysis, PS and RPP can strongly interact with TSC, resulting in a modification of its structure.
31775217	4	87	theme	TSC	692:694	arg1	film					696:699	TSC film	692:699	TSC film with 10% PS and 0.5% RPP	692:724	TSC film with 10% PS and 0.5% RPP had the highest tensile strength, and the tensile strength dropped drastically when the content of PS and RPP increased.
31775217	1	88	theme	rambutan	217:224	arg1	phenolics					231:239	rambutan peel phenolics	217:239	rambutan peel phenolics (RPP)	217:245	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31775217	1	88	theme	rambutan	217:224	arg1	RPP					242:244	RPP	242:244	RPP	242:244	Different composite films composed of tilapia skin collagen (TSC) with Pachyrhizus starch (PS) or rambutan peel phenolics (RPP) were prepared, and the physical properties of these films were determined.
31761628	4	0	theme	Langmuir	663:670	arg1	model					681:685	Langmuir isotherm model	663:685	Langmuir isotherm model	663:685	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	3	1	from	effects	418:424	arg1	performance					549:559	the adsorption performance	534:559	the adsorption performance of Mg/N-C composites in the MO removal	534:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	2	theme	temperature	468:478	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	6	3	theme	physisorption	896:908	arg1	process					851:857	The adsorption process	836:857	The adsorption process of 2-Mg/N-C-b2	836:872	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	6	3	theme	physisorption	896:908	arg1	process					910:916	a spontaneous and physisorption process	878:916	a spontaneous and physisorption process	878:916	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	1	4	attach	derived	125:131	arg1	bagasse					148:154	sugarcane bagasse	138:154	sugarcane bagasse	138:154	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	1	4	attach	derived	125:131	arg2	carbon					109:114	MgO/N-doped active carbon	90:114	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse	90:154	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	1	4	attach	derived	125:131	arg2	Mg/N-C					117:122	Mg/N-C	117:122	Mg/N-C	117:122	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	3	5	theme	pH	481:482	arg1	value					484:488	pH value	481:488	pH value	481:488	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	6	theme	ions	501:504	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	7	theme	temperature	519:529	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	2	8	theme	N-doped	298:304	arg1	carbon					313:318	N-doped active carbon	298:318	N-doped active carbon	298:318	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	6	9	theme	spontaneous	880:890	arg1	process					851:857	The adsorption process	836:857	The adsorption process of 2-Mg/N-C-b2	836:872	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	6	9	theme	spontaneous	880:890	arg1	process					910:916	a spontaneous and physisorption process	878:916	a spontaneous and physisorption process	878:916	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	2	10	theme	that	272:275	arg1	384.61 mg g-1					399:411	384.61 mg g-1	399:411	384.61 mg g-1	399:411	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	2	10	theme	that	272:275	arg1	capacity					353:360	the maximum adsorption capacity	330:360	the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal	330:394	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	3	11	theme	content	449:455	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	6	12	theme	2-Mg/N-C-b2	862:872	arg1	process					910:916	a spontaneous and physisorption process	878:916	a spontaneous and physisorption process	878:916	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	6	12	theme	2-Mg/N-C-b2	862:872	arg1	process					851:857	The adsorption process	836:857	The adsorption process of 2-Mg/N-C-b2	836:872	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	5	13	theme	intra-particle	810:823	arg1	diffusion					825:833	intra-particle diffusion	810:833	intra-particle diffusion	810:833	The rate-determining step was the boundary diffusion and intra-particle diffusion.
31761628	4	14	theme	Mg/N-C	745:750	arg1	isotherms					732:740	isotherms	732:740	isotherms	732:740	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	4	14	theme	Mg/N-C	745:750	arg1	kinetics					719:726	the adsorption kinetics	704:726	the adsorption kinetics	704:726	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	3	15	theme	inorganic	491:499	arg1	ions					501:504	inorganic ions	491:504	inorganic ions	491:504	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	2	16	theme	2-Mg/N-C-b2	365:375	arg1	capacity					353:360	the maximum adsorption capacity	330:360	the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal	330:394	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	2	16	theme	2-Mg/N-C-b2	365:375	arg1	384.61 mg g-1					399:411	384.61 mg g-1	399:411	384.61 mg g-1	399:411	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	0	17	theme	adsorption	9:18	arg1	capacity					20:27	Enhanced adsorption capacity	0:27	Enhanced adsorption capacity of MgO/N-doped active carbon	0:56	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	3	18	theme	value	484:488	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	1	19	theme	MgO/N-doped	90:100	arg1	Mg/N-C					117:122	Mg/N-C	117:122	Mg/N-C	117:122	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	1	19	theme	MgO/N-doped	90:100	arg1	carbon					109:114	MgO/N-doped active carbon	90:114	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse	90:154	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	4	20	theme	pseudo-second	633:645	arg1	model					653:657	The pseudo-second order model	629:657	The pseudo-second order model	629:657	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	0	21	theme	Enhanced	0:7	arg1	capacity					20:27	Enhanced adsorption capacity	0:27	Enhanced adsorption capacity of MgO/N-doped active carbon	0:56	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	1	22	theme	active	102:107	arg1	Mg/N-C					117:122	Mg/N-C	117:122	Mg/N-C	117:122	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	1	22	theme	active	102:107	arg1	carbon					109:114	MgO/N-doped active carbon	90:114	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse	90:154	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	5	23	theme	boundary	787:794	arg1	step					774:777	The rate-determining step	753:777	The rate-determining step	753:777	The rate-determining step was the boundary diffusion and intra-particle diffusion.
31761628	5	23	theme	boundary	787:794	arg1	diffusion					796:804	the boundary diffusion	783:804	the boundary diffusion	783:804	The rate-determining step was the boundary diffusion and intra-particle diffusion.
31761628	3	24	theme	solution	510:517	arg1	temperature					519:529	solution temperature	510:529	solution temperature	510:529	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	25	from	performance	549:559	arg1	removal					592:598	the MO removal	585:598	the MO removal	585:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	1	26	theme	methyl	188:193	arg1	orange					195:200	methyl orange	188:200	methyl orange (MO)	188:205	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	1	26	theme	methyl	188:193	arg1	MO					203:204	MO	203:204	MO	203:204	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	2	27	theme	active	280:285	arg1	carbon					287:292	active carbon	280:292	active carbon	280:292	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	1	28	theme	orange	195:200	arg1	removal					177:183	the removal	173:183	the removal of methyl orange (MO)	173:205	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	0	29	theme	active	44:49	arg1	carbon					51:56	MgO/N-doped active carbon	32:56	MgO/N-doped active carbon	32:56	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	4	30	theme	isotherm	672:679	arg1	model					681:685	Langmuir isotherm model	663:685	Langmuir isotherm model	663:685	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	2	31	theme	MO	385:386	arg1	removal					388:394	the MO removal	381:394	the MO removal	381:394	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	0	32	theme	MgO/N-doped	32:42	arg1	carbon					51:56	MgO/N-doped active carbon	32:56	MgO/N-doped active carbon	32:56	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	3	33	theme	MO	589:590	arg1	removal					592:598	the MO removal	585:598	the MO removal	585:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	6	34	theme	adsorption	840:849	arg1	process					910:916	a spontaneous and physisorption process	878:916	a spontaneous and physisorption process	878:916	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	6	34	theme	adsorption	840:849	arg1	process					851:857	The adsorption process	836:857	The adsorption process of 2-Mg/N-C-b2	836:872	The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.
31761628	2	35	theme	Mg/N-C	208:213	arg1	composites					215:224	Mg/N-C composites	208:224	Mg/N-C composites	208:224	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	3	36	theme	MgO	429:431	arg1	dosage					433:438	MgO dosage	429:438	MgO dosage	429:438	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	5	37	theme	rate-determining	757:772	arg1	step					774:777	The rate-determining step	753:777	The rate-determining step	753:777	The rate-determining step was the boundary diffusion and intra-particle diffusion.
31761628	5	37	theme	rate-determining	757:772	arg1	diffusion					796:804	the boundary diffusion	783:804	the boundary diffusion	783:804	The rate-determining step was the boundary diffusion and intra-particle diffusion.
31761628	3	38	theme	adsorption	538:547	arg1	performance					549:559	the adsorption performance	534:559	the adsorption performance of Mg/N-C composites in the MO removal	534:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	0	39	theme	carbon	51:56	arg1	capacity					20:27	Enhanced adsorption capacity	0:27	Enhanced adsorption capacity of MgO/N-doped active carbon	0:56	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	3	40	theme	pyrolysis	458:466	arg1	temperature					468:478	pyrolysis temperature	458:478	pyrolysis temperature	458:478	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	1	41	theme	sugarcane	138:146	arg1	bagasse					148:154	sugarcane bagasse	138:154	sugarcane bagasse	138:154	MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO).
31761628	2	42	theme	adsorption	247:256	arg1	capacity					258:265	the better adsorption capacity	236:265	the better adsorption capacity	236:265	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	3	43	theme	Mg/N-C	564:569	arg1	composites					571:580	Mg/N-C composites	564:580	Mg/N-C composites	564:580	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	44	theme	N-doped	441:447	arg1	content					449:455	N-doped content	441:455	N-doped content	441:455	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	2	45	theme	better	240:245	arg1	capacity					258:265	the better adsorption capacity	236:265	the better adsorption capacity	236:265	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	4	46	theme	adsorption	708:717	arg1	kinetics					719:726	the adsorption kinetics	704:726	the adsorption kinetics	704:726	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	2	47	theme	active	306:311	arg1	carbon					313:318	N-doped active carbon	298:318	N-doped active carbon	298:318	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	0	48	theme	sugarcane	71:79	arg1	bagasse					81:87	sugarcane bagasse	71:87	sugarcane bagasse	71:87	Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.
31761628	3	49	theme	composites	571:580	arg1	performance					549:559	the adsorption performance	534:559	the adsorption performance of Mg/N-C composites in the MO removal	534:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	3	50	theme	dosage	433:438	arg1	effects					418:424	The effects	414:424	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal	414:598	The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail.
31761628	2	51	theme	adsorption	342:351	arg1	capacity					353:360	the maximum adsorption capacity	330:360	the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal	330:394	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	2	51	theme	adsorption	342:351	arg1	384.61 mg g-1					399:411	384.61 mg g-1	399:411	384.61 mg g-1	399:411	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	4	52	theme	order	647:651	arg1	model					653:657	The pseudo-second order model	629:657	The pseudo-second order model	629:657	The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C.
31761628	2	53	theme	maximum	334:340	arg1	capacity					353:360	the maximum adsorption capacity	330:360	the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal	330:394	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31761628	2	53	theme	maximum	334:340	arg1	384.61 mg g-1					399:411	384.61 mg g-1	399:411	384.61 mg g-1	399:411	Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1.
31751695	0	0	theme	microporous	102:112	arg1	films					126:130	microporous photophobic films	102:130	microporous photophobic films	102:130	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	3	1	theme	larger	686:691	arg1	size					698:701	larger pore size	686:701	larger pore size	686:701	Composite films with higher SC content showed larger pore size and whiteness.
31751695	6	2	theme	peroxide	1075:1082	arg1	value					1084:1088	the lowest peroxide value	1064:1088	the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1064:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	6	3	theme	HPMC/SC/LTP	971:981	arg1	films					995:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	2	4	theme	X-ray	464:468	arg1	diffraction					470:480	wide angel X-ray diffraction	453:480	wide angel X-ray diffraction	453:480	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	3	5	theme	SC	668:669	arg1	content					671:677	higher SC content	661:677	higher SC content	661:677	Composite films with higher SC content showed larger pore size and whiteness.
31751695	2	6	theme	ultraviolet	567:577	arg1	spectrophotometer					579:595	ultraviolet spectrophotometer	567:595	ultraviolet spectrophotometer	567:595	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	1	7	theme	SC	186:187	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	7	theme	SC	186:187	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	4	8	dep	increased	770:778	arg1	decreased					785:793	decreased	785:793	increased then decreased	770:793	With the increasing SC content, crystallinity first increased then decreased.
31751695	1	9	theme	micron	266:271	arg1	scale					273:277	micron scale	266:277	micron scale to nanometer scale	266:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	0	10	theme	photophobic	114:124	arg1	films					126:130	microporous photophobic films	102:130	microporous photophobic films	102:130	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	2	11	theme	value	628:632	arg1	test					634:637	peroxide value test	619:637	peroxide value test	619:637	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	4	12	theme	SC	738:739	arg1	content					741:747	the increasing SC content	723:747	the increasing SC content	723:747	With the increasing SC content, crystallinity first increased then decreased.
31751695	6	13	theme	peanut	1093:1098	arg1	oil					1100:1102	peanut oil	1093:1102	peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1093:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	2	14	theme	X-ray	495:499	arg1	SAXS					513:516	SAXS	513:516	SAXS	513:516	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	14	theme	X-ray	495:499	arg1	scattering					501:510	small angle X-ray scattering	483:510	small angle X-ray scattering (SAXS)	483:517	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	15	dep	meaning	1152:1158	arg1	showed					1170:1175	showed	1170:1175	showed the best sustained antioxidant property	1170:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	5	16	theme	tensile	910:916	arg1	strength					918:925	tensile strength	910:925	tensile strength	910:925	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	2	17	theme	peroxide	619:626	arg1	test					634:637	peroxide value test	619:637	peroxide value test	619:637	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	18	contain	possessed	1001:1009	arg1	films					995:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	6	18	contain	possessed	1001:1009	arg2	property					1027:1034	control-release property	1011:1034	control-release property in oil system	1011:1048	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	3	19	with	films	650:654	arg1	content					671:677	higher SC content	661:677	higher SC content	661:677	Composite films with higher SC content showed larger pore size and whiteness.
31751695	6	20	theme	oil	1039:1041	arg1	system					1043:1048	oil system	1039:1048	oil system	1039:1048	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	2	21	theme	angle	489:493	arg1	SAXS					513:516	SAXS	513:516	SAXS	513:516	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	21	theme	angle	489:493	arg1	scattering					501:510	small angle X-ray scattering	483:510	small angle X-ray scattering (SAXS)	483:517	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	3	22	theme	pore	693:696	arg1	size					698:701	larger pore size	686:701	larger pore size	686:701	Composite films with higher SC content showed larger pore size and whiteness.
31751695	2	23	theme	angel	458:462	arg1	diffraction					470:480	wide angel X-ray diffraction	453:480	wide angel X-ray diffraction	453:480	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	24	theme	lowest	1068:1073	arg1	value					1084:1088	the lowest peroxide value	1064:1088	the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1064:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	1	25	theme	/lipid	189:194	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	25	theme	/lipid	189:194	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	2	26	theme	texture	598:604	arg1	analyzer					606:613	texture analyzer	598:613	texture analyzer	598:613	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	27	theme	small	483:487	arg1	SAXS					513:516	SAXS	513:516	SAXS	513:516	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	27	theme	small	483:487	arg1	scattering					501:510	small angle X-ray scattering	483:510	small angle X-ray scattering (SAXS)	483:517	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	28	theme	%	1127:1127	arg1	SC					1129:1130	3% SC	1126:1130	3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1126:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	1	29	theme	tea	196:198	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	29	theme	tea	196:198	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	6	30	theme	antioxidant	1196:1206	arg1	property					1208:1215	the best sustained antioxidant property	1177:1215	the best sustained antioxidant property	1177:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	5	31	theme	surface	833:839	arg1	Ds					829:830	the Ds	825:830	the Ds (surface fractal dimension) value	825:864	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	31	theme	surface	833:839	arg1	dimension					849:857	surface fractal dimension	833:857	surface fractal dimension	833:857	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	6	32	from	property	1027:1034	arg1	system					1043:1048	oil system	1039:1048	oil system	1039:1048	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	1	33	theme	polyphenol	200:209	arg1	films					229:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films	133:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	2	34	theme	films	425:429	arg1	microstructures					353:367	The microstructures	349:367	The microstructures	349:367	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	34	theme	films	425:429	arg1	properties					405:414	physical and sustained antioxidant properties	370:414	physical and sustained antioxidant properties of these films	370:429	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	1	35	theme	nanometer	282:290	arg1	scale					292:296	nanometer scale	282:296	nanometer scale	282:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	2	36	theme	scanning	520:527	arg1	microscope					538:547	scanning electron microscope	520:547	scanning electron microscope	520:547	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	4	37	theme	increasing	727:736	arg1	content					741:747	the increasing SC content	723:747	the increasing SC content	723:747	With the increasing SC content, crystallinity first increased then decreased.
31751695	5	38	theme	SC	812:813	arg1	addition					800:807	The addition	796:807	The addition of SC	796:813	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	0	39	theme	physical	17:24	arg1	Microstructures					0:14	Microstructures	0:14	Microstructures	0:14	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	0	39	theme	physical	17:24	arg1	properties					52:61	physical and sustained antioxidant properties	17:61	physical and sustained antioxidant properties of hydroxypropyl methylcellulose	17:94	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	1	40	from	scale	273:277	arg1	films					229:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films	133:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	41	theme	Hydroxypropyl	133:145	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	41	theme	Hydroxypropyl	133:145	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	6	42	theme	3	1126:1126	arg1	%					1127:1127	%	1127:1127	%	1127:1127	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	0	43	theme	antioxidant	40:50	arg1	Microstructures					0:14	Microstructures	0:14	Microstructures	0:14	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	0	43	theme	antioxidant	40:50	arg1	properties					52:61	physical and sustained antioxidant properties	17:61	physical and sustained antioxidant properties of hydroxypropyl methylcellulose	17:94	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	6	44	theme	sustained	1186:1194	arg1	property					1208:1215	the best sustained antioxidant property	1177:1215	the best sustained antioxidant property	1177:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	2	45	theme	antioxidant	393:403	arg1	microstructures					353:367	The microstructures	349:367	The microstructures	349:367	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	45	theme	antioxidant	393:403	arg1	properties					405:414	physical and sustained antioxidant properties	370:414	physical and sustained antioxidant properties of these films	370:429	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	0	46	theme	sustained	30:38	arg1	Microstructures					0:14	Microstructures	0:14	Microstructures	0:14	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	0	46	theme	sustained	30:38	arg1	properties					52:61	physical and sustained antioxidant properties	17:61	physical and sustained antioxidant properties of hydroxypropyl methylcellulose	17:94	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	2	47	theme	wide	453:456	arg1	diffraction					470:480	wide angel X-ray diffraction	453:480	wide angel X-ray diffraction	453:480	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	48	theme	best	1181:1184	arg1	property					1208:1215	the best sustained antioxidant property	1177:1215	the best sustained antioxidant property	1177:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	5	49	theme	Ds	829:830	arg1	value					860:864	the Ds (surface fractal dimension) value	825:864	the Ds (surface fractal dimension) value	825:864	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	1	50	theme	photophobic	217:227	arg1	films					229:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films	133:233	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	5	51	theme	fractal	841:847	arg1	Ds					829:830	the Ds	825:830	the Ds (surface fractal dimension) value	825:864	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	51	theme	fractal	841:847	arg1	dimension					849:857	surface fractal dimension	833:857	surface fractal dimension	833:857	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	2	52	theme	whiteness	550:558	arg1	meter					560:564	whiteness meter	550:564	whiteness meter	550:564	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	6	53	theme	oil	1100:1102	arg1	value					1084:1088	the lowest peroxide value	1064:1088	the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1064:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	1	54	theme	methylcellulose	147:161	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	54	theme	methylcellulose	147:161	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	6	55	with	film	1116:1119	arg1	SC					1129:1130	3% SC	1126:1130	3% SC during three weeks, meaning this film showed the best sustained antioxidant property	1126:1215	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	3	56	theme	higher	661:666	arg1	content					671:677	higher SC content	661:677	higher SC content	661:677	Composite films with higher SC content showed larger pore size and whiteness.
31751695	5	57	theme	composite	954:962	arg1	films					964:968	composite films	954:968	composite films	954:968	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	1	58	theme	HPMC	164:167	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	58	theme	HPMC	164:167	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	6	59	theme	control-release	1011:1025	arg1	property					1027:1034	control-release property	1011:1034	control-release property in oil system	1011:1048	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	2	60	theme	electron	529:536	arg1	microscope					538:547	scanning electron microscope	520:547	scanning electron microscope	520:547	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	3	61	theme	Composite	640:648	arg1	films					650:654	Composite films	640:654	Composite films with higher SC content	640:677	Composite films with higher SC content showed larger pore size and whiteness.
31751695	5	62	theme	films	964:968	arg1	strength					918:925	tensile strength	910:925	tensile strength	910:925	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	62	theme	films	964:968	arg1	smoothness					867:876	smoothness	867:876	smoothness of the cross-section structure	867:907	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	62	theme	films	964:968	arg1	modulus					943:949	modulus	943:949	modulus	943:949	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	62	theme	films	964:968	arg1	value					860:864	the Ds (surface fractal dimension) value	825:864	the Ds (surface fractal dimension) value	825:864	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	62	theme	films	964:968	arg1	elongation					928:937	elongation	928:937	elongation	928:937	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	63	theme	structure	899:907	arg1	strength					918:925	tensile strength	910:925	tensile strength	910:925	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	63	theme	structure	899:907	arg1	smoothness					867:876	smoothness	867:876	smoothness of the cross-section structure	867:907	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	63	theme	structure	899:907	arg1	modulus					943:949	modulus	943:949	modulus	943:949	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	63	theme	structure	899:907	arg1	value					860:864	the Ds (surface fractal dimension) value	825:864	the Ds (surface fractal dimension) value	825:864	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	63	theme	structure	899:907	arg1	elongation					928:937	elongation	928:937	elongation	928:937	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	5	64	theme	cross-section	885:897	arg1	structure					899:907	the cross-section structure	881:907	the cross-section structure	881:907	The addition of SC decreased the Ds (surface fractal dimension) value, smoothness of the cross-section structure, tensile strength, elongation and modulus of composite films.
31751695	0	65	theme	methylcellulose	80:94	arg1	Microstructures					0:14	Microstructures	0:14	Microstructures	0:14	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	0	65	theme	methylcellulose	80:94	arg1	properties					52:61	physical and sustained antioxidant properties	17:61	physical and sustained antioxidant properties of hydroxypropyl methylcellulose	17:94	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	2	66	theme	sustained	383:391	arg1	microstructures					353:367	The microstructures	349:367	The microstructures	349:367	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	66	theme	sustained	383:391	arg1	properties					405:414	physical and sustained antioxidant properties	370:414	physical and sustained antioxidant properties of these films	370:429	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	1	67	theme	/sodium	169:175	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	67	theme	/sodium	169:175	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	2	68	theme	physical	370:377	arg1	microstructures					353:367	The microstructures	349:367	The microstructures	349:367	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	2	68	theme	physical	370:377	arg1	properties					405:414	physical and sustained antioxidant properties	370:414	physical and sustained antioxidant properties of these films	370:429	The microstructures, physical and sustained antioxidant properties of these films were studied by using wide angel X-ray diffraction, small angle X-ray scattering (SAXS), scanning electron microscope, whiteness meter, ultraviolet spectrophotometer, texture analyzer and peroxide value test.
31751695	1	69	theme	different	240:248	arg1	sizes					255:259	different pore sizes	240:259	different pore sizes	240:259	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	0	70	theme	hydroxypropyl	66:78	arg1	methylcellulose					80:94	hydroxypropyl methylcellulose	66:94	hydroxypropyl methylcellulose	66:94	Microstructures, physical and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous photophobic films.
31751695	1	71	theme	SC	330:331	arg1	content					333:339	the SC content	326:339	the SC content (1-7%)	326:346	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	71	theme	SC	330:331	arg1	%					345:345	1-7%	342:345	1-7%	342:345	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	6	72	theme	microporous	983:993	arg1	films					995:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films	971:999	HPMC/SC/LTP microporous films possessed control-release property in oil system, reflected by the lowest peroxide value of peanut oil enclosed in film with 3% SC during three weeks, meaning this film showed the best sustained antioxidant property.
31751695	1	73	with	films	229:233	arg1	sizes					255:259	different pore sizes	240:259	different pore sizes	240:259	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	74	theme	citrate	177:183	arg1	LTP					212:214	LTP	212:214	LTP	212:214	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	74	theme	citrate	177:183	arg1	polyphenol					200:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol	133:209	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale	133:296	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
31751695	1	75	theme	pore	250:253	arg1	sizes					255:259	different pore sizes	240:259	different pore sizes	240:259	Hydroxypropyl methylcellulose (HPMC)/sodium citrate (SC)/lipid tea polyphenol (LTP) photophobic films with different pore sizes from micron scale to nanometer scale were prepared by regulating the SC content (1-7%).
30678924	1	0	theme	ternary	193:199	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	1	1	theme	orthopedic	290:299	arg1	application					301:311	orthopedic application	290:311	orthopedic application	290:311	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	7	2	theme	synthesized	945:955	arg1	composite					957:965	synthesized composite	945:965	synthesized composite	945:965	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	4	3	dep	strength	607:614	arg1	the					594:596	the	594:596	the	594:596	Consequently, the flexural strength and compressive strength of composite have reached to 140 MPa and 118 MPa, respectively.
30678924	5	4	theme	composite	733:741	arg1	modulus					718:724	The flexural modulus	705:724	The flexural modulus of the composite	705:741	The flexural modulus of the composite is in the range of 9-22 GPa.
30678924	0	5	theme	bioactive	108:116	arg1	graft					123:127	A mechanically strong bioactive bone graft	86:127	A mechanically strong bioactive bone graft	86:127	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	0	6	theme	strong	101:106	arg1	graft					123:127	A mechanically strong bioactive bone graft	86:127	A mechanically strong bioactive bone graft	86:127	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	1	7	theme	reinforced	143:152	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	3	8	theme	functional	413:422	arg1	groups					424:429	The functional groups	409:429	The functional groups onto the surface of fibers	409:456	The functional groups onto the surface of fibers induced the formation of hydroxyapatite at the bridging position through which fibers were effectively bound with matrix.
30678924	6	9	theme	cell	781:784	arg1	study					786:790	In-vitro cell study	772:790	In-vitro cell study	772:790	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	3	10	theme	bridging	505:512	arg1	position					514:521	the bridging position	501:521	the bridging position through which fibers were effectively bound with matrix	501:577	The functional groups onto the surface of fibers induced the formation of hydroxyapatite at the bridging position through which fibers were effectively bound with matrix.
30678924	0	11	theme	bone	118:121	arg1	graft					123:127	A mechanically strong bioactive bone graft	86:127	A mechanically strong bioactive bone graft	86:127	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	5	12	theme	flexural	709:716	arg1	modulus					718:724	The flexural modulus	705:724	The flexural modulus of the composite	705:741	The flexural modulus of the composite is in the range of 9-22 GPa.
30678924	6	13	theme	In-vitro	772:779	arg1	study					786:790	In-vitro cell study	772:790	In-vitro cell study	772:790	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	3	14	theme	fibers	451:456	arg1	surface					440:446	the surface	436:446	the surface of fibers	436:456	The functional groups onto the surface of fibers induced the formation of hydroxyapatite at the bridging position through which fibers were effectively bound with matrix.
30678924	2	15	theme	functionalized	379:392	arg1	fibers					401:406	chemically functionalized carbon fibers	368:406	chemically functionalized carbon fibers	368:406	Composites were synthesized with the incorporation of chemically functionalized carbon fibers.
30678924	0	16	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of carbon fiber	0:31	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	7	17	theme	excellent	897:905	arg1	properties					933:942	these excellent mechanical and biological properties	891:942	these excellent mechanical and biological properties	891:942	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	6	18	theme	differentiation	861:875	arg1	ability					877:883	excellent cell proliferation and differentiation ability	828:883	excellent cell proliferation and differentiation ability	828:883	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	6	19	contain	possesses	818:826	arg1	composite					808:816	the composite	804:816	the composite	804:816	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	6	19	contain	possesses	818:826	arg2	ability					877:883	excellent cell proliferation and differentiation ability	828:883	excellent cell proliferation and differentiation ability	828:883	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	0	20	theme	carbon	20:25	arg1	fiber					27:31	carbon fiber	20:31	carbon fiber	20:31	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	7	21	theme	bone	1036:1039	arg1	graft					1041:1045	a mechanically compatible bioactive bone graft	1000:1045	a mechanically compatible bioactive bone graft	1000:1045	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	1	22	theme	simple	239:244	arg1	method					264:269	a simple wet precipitation method	237:269	a simple wet precipitation method for weight bearing orthopedic application	237:311	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	4	23	theme	composite	644:652	arg1	strength					607:614	flexural strength	598:614	flexural strength	598:614	Consequently, the flexural strength and compressive strength of composite have reached to 140 MPa and 118 MPa, respectively.
30678924	4	23	theme	composite	644:652	arg1	strength					632:639	compressive strength	620:639	compressive strength	620:639	Consequently, the flexural strength and compressive strength of composite have reached to 140 MPa and 118 MPa, respectively.
30678924	7	24	theme	biological	922:931	arg1	properties					933:942	these excellent mechanical and biological properties	891:942	these excellent mechanical and biological properties	891:942	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	1	25	theme	wet	246:248	arg1	method					264:269	a simple wet precipitation method	237:269	a simple wet precipitation method for weight bearing orthopedic application	237:311	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	0	26	theme	fiber	27:31	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of carbon fiber	0:31	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	3	27	theme	hydroxyapatite	483:496	arg1	formation					470:478	the formation	466:478	the formation of hydroxyapatite	466:496	The functional groups onto the surface of fibers induced the formation of hydroxyapatite at the bridging position through which fibers were effectively bound with matrix.
30678924	6	28	theme	proliferation	843:855	arg1	ability					877:883	excellent cell proliferation and differentiation ability	828:883	excellent cell proliferation and differentiation ability	828:883	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	1	29	theme	precipitation	250:262	arg1	method					264:269	a simple wet precipitation method	237:269	a simple wet precipitation method for weight bearing orthopedic application	237:311	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	0	30	theme	ternary	67:73	arg1	composite					75:83	polymer-hydroxyapatite ternary composite	44:83	polymer-hydroxyapatite ternary composite	44:83	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	4	31	theme	flexural	598:605	arg1	strength					607:614	flexural strength	598:614	flexural strength	598:614	Consequently, the flexural strength and compressive strength of composite have reached to 140 MPa and 118 MPa, respectively.
30678924	7	32	theme	mechanical	907:916	arg1	properties					933:942	these excellent mechanical and biological properties	891:942	these excellent mechanical and biological properties	891:942	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	6	33	theme	cell	838:841	arg1	proliferation					843:855	excellent cell proliferation	828:855	excellent cell proliferation	828:855	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	0	34	theme	polymer-hydroxyapatite	44:65	arg1	composite					75:83	polymer-hydroxyapatite ternary composite	44:83	polymer-hydroxyapatite ternary composite	44:83	Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: A mechanically strong bioactive bone graft.
30678924	4	35	theme	compressive	620:630	arg1	strength					632:639	compressive strength	620:639	compressive strength	620:639	Consequently, the flexural strength and compressive strength of composite have reached to 140 MPa and 118 MPa, respectively.
30678924	6	36	theme	excellent	828:836	arg1	proliferation					843:855	excellent cell proliferation	828:855	excellent cell proliferation	828:855	In-vitro cell study showed that the composite possesses excellent cell proliferation and differentiation ability.
30678924	1	37	theme	Carbon	130:135	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	7	38	theme	compatible	1015:1024	arg1	graft					1041:1045	a mechanically compatible bioactive bone graft	1000:1045	a mechanically compatible bioactive bone graft	1000:1045	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	1	39	theme	fiber	137:141	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	7	40	theme	bioactive	1026:1034	arg1	graft					1041:1045	a mechanically compatible bioactive bone graft	1000:1045	a mechanically compatible bioactive bone graft	1000:1045	With these excellent mechanical and biological properties, synthesized composite exhibits potential to be used as a mechanically compatible bioactive bone graft.
30678924	5	41	theme	9-22 GPa	762:769	arg1	range					753:757	the range	749:757	the range of 9-22 GPa	749:769	The flexural modulus of the composite is in the range of 9-22 GPa.
30678924	2	42	theme	carbon	394:399	arg1	fibers					401:406	chemically functionalized carbon fibers	368:406	chemically functionalized carbon fibers	368:406	Composites were synthesized with the incorporation of chemically functionalized carbon fibers.
30678924	2	43	theme	fibers	401:406	arg1	incorporation					351:363	the incorporation	347:363	the incorporation of chemically functionalized carbon fibers	347:406	Composites were synthesized with the incorporation of chemically functionalized carbon fibers.
30678924	1	44	theme	carboxymethyl	154:166	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
30678924	1	45	theme	cellulose-hydroxyapatite	168:191	arg1	composites					201:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites	130:210	Carbon fiber reinforced carboxymethyl cellulose-hydroxyapatite ternary composites have been synthesized by a simple wet precipitation method for weight bearing orthopedic application.
29910225	1	0	theme	intramammary	136:147	arg1	infusion					149:156	intramammary infusion	136:156	intramammary infusion of Escherichia coli lipopolysaccharide (LPS)	136:201	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	0	1	theme	udder	75:79	arg1	pH					59:60	milk pH	54:60	milk pH of uninfused udder in goat	54:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	3	2	theme	Clinical	332:339	arg1	temperature					364:374	rectal temperature	357:374	rectal temperature	357:374	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	2	theme	Clinical	332:339	arg1	activity					389:396	physical activity	380:396	physical activity	380:396	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	2	theme	Clinical	332:339	arg1	manifestations					341:354	Clinical manifestations	332:354	Clinical manifestations (rectal temperature and physical activity)	332:397	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	0	3	from	Effect	0:5	arg1	pH					59:60	milk pH	54:60	milk pH of uninfused udder in goat	54:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	4	4	from	udders	679:684	arg1	pH					658:659	the pH	654:659	the pH of milk from both udders	654:684	After intramammary LPS challenge, the pH of milk from both udders increased.
29910225	0	5	from	pH	59:60	arg1	goat					84:87	goat	84:87	goat	84:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	3	6	theme	milk	462:465	arg1	compositions					467:478	milk compositions	462:478	milk compositions (somatic cell count and pH)	462:506	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	6	theme	milk	462:465	arg1	pH					504:505	pH	504:505	pH	504:505	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	6	theme	milk	462:465	arg1	count					494:498	somatic cell count	481:498	somatic cell count	481:498	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	4	7	theme	LPS	639:641	arg1	challenge					643:651	intramammary LPS challenge	626:651	intramammary LPS challenge	626:651	After intramammary LPS challenge, the pH of milk from both udders increased.
29910225	3	8	dep	challenge	544:552	arg1	just					532:535	just	532:535	just	532:535	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	9	theme	cell	489:492	arg1	compositions					467:478	milk compositions	462:478	milk compositions (somatic cell count and pH)	462:506	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	9	theme	cell	489:492	arg1	count					494:498	somatic cell count	481:498	somatic cell count	481:498	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	10	theme	selected	400:407	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	10	theme	selected	400:407	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	10	theme	selected	400:407	arg1	pH					427:428	pH	427:428	pH	427:428	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	1	11	theme	Escherichia	161:171	arg1	LPS					198:200	LPS	198:200	LPS	198:200	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	1	11	theme	Escherichia	161:171	arg1	lipopolysaccharide					178:195	lipopolysaccharide	178:195	Escherichia coli lipopolysaccharide (LPS)	161:201	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	2	12	theme	intramammary	285:296	arg1	administration					298:311	intramammary administration	285:311	intramammary administration to the left udder	285:329	Four clinically healthy goats were infused with LPS (100 µg) by intramammary administration to the left udder.
29910225	3	13	theme	post-challenge	604:617	arg1	hr					601:602	the first 24 hr post-challenge	588:617	the first 24 hr post-challenge	588:617	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	14	dep	compositions	467:478	arg1	compositions					467:478	milk compositions	462:478	milk compositions (somatic cell count and pH)	462:506	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	14	dep	compositions	467:478	arg1	pH					504:505	pH	504:505	pH	504:505	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	14	dep	compositions	467:478	arg1	count					494:498	somatic cell count	481:498	somatic cell count	481:498	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	15	theme	blood	409:413	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	15	theme	blood	409:413	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	15	theme	blood	409:413	arg1	pH					427:428	pH	427:428	pH	427:428	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	0	16	theme	lipopolysaccharide	23:40	arg1	infusion					42:49	intramammary lipopolysaccharide infusion	10:49	intramammary lipopolysaccharide infusion	10:49	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	4	17	theme	milk	664:667	arg1	pH					658:659	the pH	654:659	the pH of milk from both udders	654:684	After intramammary LPS challenge, the pH of milk from both udders increased.
29910225	3	18	theme	white	434:438	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	18	theme	white	434:438	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	0	19	theme	intramammary	10:21	arg1	infusion					42:49	intramammary lipopolysaccharide infusion	10:49	intramammary lipopolysaccharide infusion	10:49	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	3	20	theme	time	571:574	arg1	points					576:581	multiple time points	562:581	multiple time points over the first 24 hr post-challenge	562:617	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	21	theme	blood	440:444	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	21	theme	blood	440:444	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	4	22	theme	intramammary	626:637	arg1	challenge					643:651	intramammary LPS challenge	626:651	intramammary LPS challenge	626:651	After intramammary LPS challenge, the pH of milk from both udders increased.
29910225	3	23	theme	rectal	357:362	arg1	temperature					364:374	rectal temperature	357:374	rectal temperature	357:374	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	23	theme	rectal	357:362	arg1	manifestations					341:354	Clinical manifestations	332:354	Clinical manifestations (rectal temperature and physical activity)	332:397	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	0	24	from	udder	75:79	arg1	goat					84:87	goat	84:87	goat	84:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	1	25	theme	lipopolysaccharide	178:195	arg1	infusion					149:156	intramammary infusion	136:156	intramammary infusion of Escherichia coli lipopolysaccharide (LPS)	136:201	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	0	26	theme	infusion	42:49	arg1	Effect					0:5	Effect	0:5	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.	0:88	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	5	27	theme	increased	771:779	arg1	pH					781:782	increased pH	771:782	increased pH in milk from the unchallenged (contralateral) udder	771:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	1	28	dep	lipopolysaccharide	178:195	arg1	coli					173:176	Escherichia coli lipopolysaccharide (LPS)	161:201	Escherichia coli lipopolysaccharide (LPS)	161:201	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	3	29	theme	physical	380:387	arg1	activity					389:396	physical activity	380:396	physical activity	380:396	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	29	theme	physical	380:387	arg1	manifestations					341:354	Clinical manifestations	332:354	Clinical manifestations (rectal temperature and physical activity)	332:397	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	30	theme	multiple	562:569	arg1	points					576:581	multiple time points	562:581	multiple time points over the first 24 hr post-challenge	562:617	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	31	dep	parameters	415:424	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	31	dep	parameters	415:424	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	31	dep	parameters	415:424	arg1	pH					427:428	pH	427:428	pH	427:428	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	2	32	theme	left	320:323	arg1	udder					325:329	the left udder	316:329	the left udder	316:329	Four clinically healthy goats were infused with LPS (100 µg) by intramammary administration to the left udder.
29910225	0	33	theme	milk	54:57	arg1	pH					59:60	milk pH	54:60	milk pH of uninfused udder in goat	54:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	3	34	theme	first	592:596	arg1	hr					601:602	the first 24 hr post-challenge	588:617	the first 24 hr post-challenge	588:617	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	5	35	from	mastitis	740:747	arg1	goat					752:755	goat	752:755	goat	752:755	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	1	36	theme	milk	104:107	arg1	composition					109:119	milk composition	104:119	milk composition	104:119	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	3	37	theme	cell	446:449	arg1	parameters					415:424	selected blood parameters	400:424	selected blood parameters (pH and white blood cell count)	400:456	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	37	theme	cell	446:449	arg1	count					451:455	white blood cell count	434:455	white blood cell count	434:455	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	1	38	from	change	94:99	arg1	composition					109:119	milk composition	104:119	milk composition	104:119	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	1	38	from	change	94:99	arg1	response					124:131	response	124:131	response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS)	124:201	The change in milk composition in response to intramammary infusion of Escherichia coli lipopolysaccharide (LPS) was investigated.
29910225	3	39	dep	manifestations	341:354	arg1	temperature					364:374	rectal temperature	357:374	rectal temperature	357:374	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	39	dep	manifestations	341:354	arg1	activity					389:396	physical activity	380:396	physical activity	380:396	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	39	dep	manifestations	341:354	arg1	manifestations					341:354	Clinical manifestations	332:354	Clinical manifestations (rectal temperature and physical activity)	332:397	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	5	40	theme	unchallenged	801:812	arg1	udder					830:834	the unchallenged (contralateral) udder	797:834	the unchallenged (contralateral) udder	797:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	5	41	theme	LPS-induced	728:738	arg1	mastitis					740:747	LPS-induced mastitis	728:747	LPS-induced mastitis in goat	728:755	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	5	42	from	udder	830:834	arg1	milk					787:790	milk	787:790	milk from the unchallenged (contralateral) udder	787:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	5	42	from	udder	830:834	arg1	pH					781:782	increased pH	771:782	increased pH in milk from the unchallenged (contralateral) udder	771:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	5	43	theme	contralateral	815:827	arg1	udder					830:834	the unchallenged (contralateral) udder	797:834	the unchallenged (contralateral) udder	797:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	0	44	theme	uninfused	65:73	arg1	udder					75:79	uninfused udder	65:79	uninfused udder in goat	65:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	0	45	from	goat	84:87	arg1	pH					59:60	milk pH	54:60	milk pH of uninfused udder in goat	54:87	Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat.
29910225	5	46	from	pH	781:782	arg1	udder					830:834	the unchallenged (contralateral) udder	797:834	the unchallenged (contralateral) udder	797:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	5	46	from	pH	781:782	arg1	milk					787:790	milk	787:790	milk from the unchallenged (contralateral) udder	787:834	Thus, this study revealed that LPS-induced mastitis in goat can result in increased pH in milk from the unchallenged (contralateral) udder.
29910225	2	47	theme	healthy	237:243	arg1	goats					245:249	Four clinically healthy goats	221:249	Four clinically healthy goats	221:249	Four clinically healthy goats were infused with LPS (100 µg) by intramammary administration to the left udder.
29910225	3	48	theme	somatic	481:487	arg1	compositions					467:478	milk compositions	462:478	milk compositions (somatic cell count and pH)	462:506	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
29910225	3	48	theme	somatic	481:487	arg1	count					494:498	somatic cell count	481:498	somatic cell count	481:498	Clinical manifestations (rectal temperature and physical activity), selected blood parameters (pH and white blood cell count) and milk compositions (somatic cell count and pH) were evaluated at 0 hr (just before challenge) and at multiple time points over the first 24 hr post-challenge.
31387235	0	0	theme	Hydrogel	98:105	arg1	Properties					107:116	Hydrogel Properties	98:116	Hydrogel Properties	98:116	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	0	1	from	Content	24:30	arg1	Hydrogels					35:43	Hydrogels	35:43	Hydrogels Obtained through Blue Light-Induced Gelation	35:88	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	1	2	theme	hyaluronic	174:183	arg1	acid					185:188	hyaluronic acid	174:188	hyaluronic acid	174:188	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	6	3	theme	cell	968:971	arg1	genes					980:984	Stem cell marker genes	963:984	Stem cell marker genes	963:984	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	3	theme	cell	968:971	arg1	Oct4					994:997	Oct4	994:997	Oct4	994:997	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	3	theme	cell	968:971	arg1	Sox2					1004:1007	Sox2	1004:1007	Sox2	1004:1007	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	3	theme	cell	968:971	arg1	Nanog					987:991	Nanog	987:991	Nanog	987:991	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	4	with	hydrogels	1060:1068	arg1	content					1084:1090	a higher content	1075:1090	a higher content of hyaluronic acid	1075:1109	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	7	5	theme	engineering	1372:1382	arg1	applications					1384:1395	tissue engineering applications	1365:1395	tissue engineering applications	1365:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	1	6	theme	acid	185:188	arg1	hydrogels					161:169	Composite hydrogels	151:169	Composite hydrogels of hyaluronic acid and gelatin	151:200	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	6	7	theme	Stem	963:966	arg1	genes					980:984	Stem cell marker genes	963:984	Stem cell marker genes	963:984	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	7	theme	Stem	963:966	arg1	Oct4					994:997	Oct4	994:997	Oct4	994:997	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	7	theme	Stem	963:966	arg1	Sox2					1004:1007	Sox2	1004:1007	Sox2	1004:1007	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	7	theme	Stem	963:966	arg1	Nanog					987:991	Nanog	987:991	Nanog	987:991	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	5	8	with	hydrogels	893:901	arg1	content					935:941	a higher rather than lower content	908:941	a higher rather than lower content of hyaluronic acid	908:960	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	2	9	from	useful	342:347	arg1	particular					251:260	particular	251:260	particular	251:260	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	2	9	from	useful	342:347	arg1	engineering					359:369	tissue engineering	352:369	tissue engineering	352:369	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	5	10	theme	lower	929:933	arg1	content					935:941	a higher rather than lower content	908:941	a higher rather than lower content of hyaluronic acid	908:960	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	6	11	theme	higher	1077:1082	arg1	content					1084:1090	a higher content	1075:1090	a higher content of hyaluronic acid	1075:1109	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	12	theme	culture	1187:1193	arg1	dishes					1195:1200	tissue culture dishes	1180:1200	tissue culture dishes	1180:1200	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	2	13	from	engineering	359:369	arg1	useful					342:347	useful	342:347	useful	342:347	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	2	13	from	engineering	359:369	arg1	hydrogels					277:285	the composite hydrogels	263:285	the composite hydrogels obtained through processes that are mild for cells	263:336	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	3	14	theme	polymer	562:568	arg1	content					546:552	the content	542:552	the content of each polymer in the precursor solution	542:594	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	0	15	theme	Stem	130:133	arg1	Behaviors					140:148	Adipose Stem Cell Behaviors	122:148	Adipose Stem Cell Behaviors	122:148	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	4	16	theme	kPa	824:826	arg1	modulus					836:842	0.5-1.2 kPa Young's modulus	816:842	0.5-1.2 kPa Young's modulus	816:842	Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus.
31387235	3	17	theme	content	546:552	arg1	effect					532:537	the effect	528:537	the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels	528:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	5	18	theme	Human	845:849	arg1	cells					864:868	Human adipose stem cells	845:868	Human adipose stem cells	845:868	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	0	19	theme	Adipose	122:128	arg1	Behaviors					140:148	Adipose Stem Cell Behaviors	122:148	Adipose Stem Cell Behaviors	122:148	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	4	20	theme	0.5-1.2	816:822	arg1	kPa					824:826	kPa	824:826	kPa	824:826	Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus.
31387235	3	21	theme	composite	411:419	arg1	hydrogels					421:429	hyaluronic acid/gelatin composite hydrogels	387:429	hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells	387:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	1	22	theme	gelatin	194:200	arg1	hydrogels					161:169	Composite hydrogels	151:169	Composite hydrogels of hyaluronic acid and gelatin	151:200	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	0	23	theme	Acid	19:22	arg1	Content					24:30	Gelatin/Hyaluronic Acid Content	0:30	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation	0:88	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	3	24	theme	precursor	577:585	arg1	solution					587:594	the precursor solution	573:594	the precursor solution	573:594	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	2	25	from	particular	251:260	arg1	useful					342:347	useful	342:347	useful	342:347	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	2	25	from	particular	251:260	arg1	hydrogels					277:285	the composite hydrogels	263:285	the composite hydrogels obtained through processes that are mild for cells	263:336	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	3	26	theme	blue	450:453	arg1	gelation					469:476	a blue light-induced gelation	448:476	a blue light-induced gelation that is mild for mammalian cells	448:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	26	theme	blue	450:453	arg1	mild					486:489	mild	486:489	mild	486:489	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	0	27	theme	Gelatin/Hyaluronic	0:17	arg1	Content					24:30	Gelatin/Hyaluronic Acid Content	0:30	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation	0:88	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	6	28	theme	tissue	1180:1185	arg1	dishes					1195:1200	tissue culture dishes	1180:1200	tissue culture dishes	1180:1200	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	4	29	theme	Young	828:832	arg1	modulus					836:842	0.5-1.2 kPa Young's modulus	816:842	0.5-1.2 kPa Young's modulus	816:842	Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus.
31387235	4	30	from	gelation	741:748	arg1	s					766:766	less than 20 s	753:766	less than 20 s	753:766	Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus.
31387235	5	31	theme	higher	910:915	arg1	content					935:941	a higher rather than lower content	908:941	a higher rather than lower content of hyaluronic acid	908:960	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	1	32	theme	great	210:214	arg1	attention					216:224	great attention	210:224	great attention in biomedical fields	210:245	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	7	33	theme	tissue	1365:1370	arg1	applications					1384:1395	tissue engineering applications	1365:1395	tissue engineering applications	1365:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	3	34	theme	hyaluronic	387:396	arg1	hydrogels					421:429	hyaluronic acid/gelatin composite hydrogels	387:429	hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells	387:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	0	35	theme	Cell	135:138	arg1	Behaviors					140:148	Adipose Stem Cell Behaviors	122:148	Adipose Stem Cell Behaviors	122:148	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	7	36	theme	suitable	1352:1359	arg1	gelation					1343:1350	blue light-induced gelation	1324:1350	blue light-induced gelation suitable for tissue engineering applications	1324:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	3	37	theme	mammalian	495:503	arg1	cells					505:509	mammalian cells	495:509	mammalian cells	495:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	6	38	theme	composite	1050:1058	arg1	hydrogels					1060:1068	the composite hydrogels	1046:1068	the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes	1046:1200	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	3	39	theme	human	661:665	arg1	cells					680:684	human adipose stem cells	661:684	human adipose stem cells laden in the hydrogels	661:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	7	40	theme	blue	1324:1327	arg1	gelation					1343:1350	blue light-induced gelation	1324:1350	blue light-induced gelation suitable for tissue engineering applications	1324:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	3	41	theme	light-induced	455:467	arg1	gelation					469:476	a blue light-induced gelation	448:476	a blue light-induced gelation that is mild for mammalian cells	448:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	41	theme	light-induced	455:467	arg1	mild					486:489	mild	486:489	mild	486:489	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	1	42	theme	biomedical	229:238	arg1	fields					240:245	biomedical fields	229:245	biomedical fields	229:245	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	7	43	theme	light-induced	1329:1341	arg1	gelation					1343:1350	blue light-induced gelation	1324:1350	blue light-induced gelation suitable for tissue engineering applications	1324:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	0	44	theme	Blue	62:65	arg1	Gelation					81:88	Blue Light-Induced Gelation	62:88	Blue Light-Induced Gelation	62:88	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	7	45	theme	gelatin/hyaluronic	1263:1280	arg1	hydrogels					1297:1305	gelatin/hyaluronic acid composite hydrogels	1263:1305	gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications	1263:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	6	46	theme	acid	1106:1109	arg1	content					1084:1090	a higher content	1075:1090	a higher content of hyaluronic acid	1075:1109	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	3	47	theme	cells	680:684	arg1	properties					609:618	properties	609:618	properties of resultant hydrogels	609:641	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	47	theme	cells	680:684	arg1	gelation					599:606	gelation	599:606	gelation	599:606	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	47	theme	cells	680:684	arg1	behaviors					648:656	behaviors	648:656	behaviors of human adipose stem cells laden in the hydrogels	648:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	1	48	from	attention	216:224	arg1	fields					240:245	biomedical fields	229:245	biomedical fields	229:245	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	2	49	theme	tissue	352:357	arg1	engineering					359:369	tissue engineering	352:369	tissue engineering	352:369	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	7	50	theme	acid	1282:1285	arg1	hydrogels					1297:1305	gelatin/hyaluronic acid composite hydrogels	1263:1305	gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications	1263:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	5	51	theme	acid	957:960	arg1	content					935:941	a higher rather than lower content	908:941	a higher rather than lower content of hyaluronic acid	908:960	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	3	52	theme	hydrogels	633:641	arg1	properties					609:618	properties	609:618	properties of resultant hydrogels	609:641	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	52	theme	hydrogels	633:641	arg1	gelation					599:606	gelation	599:606	gelation	599:606	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	52	theme	hydrogels	633:641	arg1	behaviors					648:656	behaviors	648:656	behaviors of human adipose stem cells laden in the hydrogels	648:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	53	from	effect	532:537	arg1	behaviors					648:656	behaviors	648:656	behaviors of human adipose stem cells laden in the hydrogels	648:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	53	from	effect	532:537	arg1	properties					609:618	properties	609:618	properties of resultant hydrogels	609:641	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	53	from	effect	532:537	arg1	gelation					599:606	gelation	599:606	gelation	599:606	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	53	from	effect	532:537	arg1	solution					587:594	the precursor solution	573:594	the precursor solution	573:594	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	4	54	theme	content	725:731	arg1	Control					710:716	Control	710:716	Control of the content	710:731	Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus.
31387235	5	55	theme	adipose	851:857	arg1	cells					864:868	Human adipose stem cells	845:868	Human adipose stem cells	845:868	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	5	56	theme	hyaluronic	946:955	arg1	acid					957:960	hyaluronic acid	946:960	hyaluronic acid	946:960	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	3	57	theme	adipose	667:673	arg1	cells					680:684	human adipose stem cells	661:684	human adipose stem cells laden in the hydrogels	661:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	7	58	theme	composite	1287:1295	arg1	hydrogels					1297:1305	gelatin/hyaluronic acid composite hydrogels	1263:1305	gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications	1263:1395	These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
31387235	6	59	theme	hyaluronic	1095:1104	arg1	acid					1106:1109	hyaluronic acid	1095:1109	hyaluronic acid	1095:1109	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	1	60	theme	Composite	151:159	arg1	hydrogels					161:169	Composite hydrogels	151:169	Composite hydrogels of hyaluronic acid and gelatin	151:200	Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields.
31387235	3	61	theme	stem	675:678	arg1	cells					680:684	human adipose stem cells	661:684	human adipose stem cells laden in the hydrogels	661:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	2	62	theme	composite	267:275	arg1	useful					342:347	useful	342:347	useful	342:347	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	2	62	theme	composite	267:275	arg1	hydrogels					277:285	the composite hydrogels	263:285	the composite hydrogels obtained through processes that are mild for cells	263:336	In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering.
31387235	5	63	theme	stem	859:862	arg1	cells					864:868	Human adipose stem cells	845:868	Human adipose stem cells	845:868	Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid.
31387235	3	64	from	solution	587:594	arg1	effect					532:537	the effect	528:537	the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels	528:707	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	0	65	theme	Light-Induced	67:79	arg1	Gelation					81:88	Blue Light-Induced Gelation	62:88	Blue Light-Induced Gelation	62:88	Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.
31387235	3	66	theme	resultant	623:631	arg1	hydrogels					633:641	resultant hydrogels	623:641	resultant hydrogels	623:641	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	6	67	from	cells	1037:1041	arg1	hydrogels					1060:1068	the composite hydrogels	1046:1068	the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes	1046:1200	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	3	68	theme	acid/gelatin	398:409	arg1	hydrogels					421:429	hyaluronic acid/gelatin composite hydrogels	387:429	hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells	387:509	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	3	69	from	content	546:552	arg1	solution					587:594	the precursor solution	573:594	the precursor solution	573:594	In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels.
31387235	6	70	theme	marker	973:978	arg1	genes					980:984	Stem cell marker genes	963:984	Stem cell marker genes	963:984	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	70	theme	marker	973:978	arg1	Oct4					994:997	Oct4	994:997	Oct4	994:997	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	70	theme	marker	973:978	arg1	Sox2					1004:1007	Sox2	1004:1007	Sox2	1004:1007	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
31387235	6	70	theme	marker	973:978	arg1	Nanog					987:991	Nanog	987:991	Nanog	987:991	Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes.
30394298	5	0	theme	R1	1124:1125	arg1	rates					1139:1143	longitudinal (R1) relaxation rates	1110:1143	longitudinal (R1) relaxation rates	1110:1143	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	1	1	theme	novel	144:148	arg1	material					162:169	novel MRI phantom material	144:169	novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties	144:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	2	2	dep	samples	473:479	arg1	230 μT					445:450	230 μT	445:450	230 μT	445:450	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	5	3	theme	relaxation	1174:1183	arg1	rates					1185:1189	transverse (R2) relaxation rates	1158:1189	transverse (R2) relaxation rates	1158:1189	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	2	4	theme	gel	469:471	arg1	samples					473:479	3 T. Nineteen gel samples	455:479	3 T. Nineteen gel samples	455:479	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	3	5	used	utilized	786:793	arg2	methylparaben					767:779	methylparaben	767:779	methylparaben	767:779	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	3	5	used	utilized	786:793	arg2	4‑hydroxybenzoate					748:764	methyl 4‑hydroxybenzoate	741:764	methyl 4‑hydroxybenzoate (methylparaben)	741:780	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	2	6	theme	interactive	584:594	arg1	effects					596:602	the linear, quadratic, and interactive effects	557:602	the linear, quadratic, and interactive effects of the contrast agents	557:625	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	7	theme	composite	640:648	arg1	design					650:655	a central composite design	630:655	a central composite design of experiment	630:669	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	1	8	theme	MRI	150:152	arg1	material					162:169	novel MRI phantom material	144:169	novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties	144:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	5	9	from	concentration	1039:1051	arg1	property					1021:1028	the intended relaxation property	997:1028	the intended relaxation property from the concentration of the contrast agent	997:1073	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	1	10	theme	tunable	238:244	arg1	properties					257:266	tunable relaxation properties	238:266	tunable relaxation properties	238:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	6	11	theme	optical	1368:1374	arg1	clarity					1376:1382	optical clarity	1368:1382	optical clarity	1368:1382	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	5	12	theme	relaxation	1128:1137	arg1	rates					1139:1143	longitudinal (R1) relaxation rates	1110:1143	longitudinal (R1) relaxation rates	1110:1143	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	2	13	theme	iron	354:357	arg1	SPIONs					380:385	SPIONs	380:385	SPIONs	380:385	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	13	theme	iron	354:357	arg1	nanoparticles					365:377	newly synthesized superparamagnetic iron oxide nanoparticles	318:377	newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs)	318:386	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	14	theme	central	632:638	arg1	design					650:655	a central composite design	630:655	a central composite design of experiment	630:669	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	5	15	theme	mathematical	947:958	arg1	model					960:964	The mathematical model	943:964	The mathematical model	943:964	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	5	15	theme	mathematical	947:958	arg1	able					970:973	able	970:973	able	970:973	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	1	16	theme	relaxation	246:255	arg1	properties					257:266	tunable relaxation properties	238:266	tunable relaxation properties	238:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	5	17	theme	R2	1089:1090	arg1	values > 0.97					1092:1104	adjusted R2 values > 0.97	1080:1104	adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates	1080:1189	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	2	18	theme	oxide	359:363	arg1	SPIONs					380:385	SPIONs	380:385	SPIONs	380:385	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	18	theme	oxide	359:363	arg1	nanoparticles					365:377	newly synthesized superparamagnetic iron oxide nanoparticles	318:377	newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs)	318:386	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	19	theme	varying	502:508	arg1	concentrations					510:523	varying concentrations	502:523	varying concentrations of contrast agents	502:542	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	20	theme	agents	620:625	arg1	effects					596:602	the linear, quadratic, and interactive effects	557:602	the linear, quadratic, and interactive effects of the contrast agents	557:625	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	1	21	theme	phantom	154:160	arg1	material					162:169	novel MRI phantom material	144:169	novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties	144:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	3	22	from	growth	693:698	arg1	gels					707:710	the gels	703:710	the gels	703:710	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	2	23	theme	contrast	611:618	arg1	agents					620:625	the contrast agents	607:625	the contrast agents	607:625	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	1	24	theme	OBJECT	75:80	arg1	research					86:93	OBJECT The research	75:93	OBJECT The research	75:93	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	5	25	theme	longitudinal	1110:1121	arg1	rates					1139:1143	longitudinal (R1) relaxation rates	1110:1143	longitudinal (R1) relaxation rates	1110:1143	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	4	26	theme	metal	836:840	arg1	salts					842:846	metal salts	836:846	metal salts	836:846	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	2	27	theme	experiment	660:669	arg1	design					650:655	a central composite design	630:655	a central composite design of experiment	630:669	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	1	28	theme	The	82:84	arg1	research					86:93	OBJECT The research	75:93	OBJECT The research	75:93	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	3	29	theme	long-term	726:734	arg1	use					736:738	long-term use	726:738	long-term use	726:738	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	6	30	theme	physical	1231:1238	arg1	stability					1266:1274	physical, chemical, and biological stability	1231:1274	physical, chemical, and biological stability	1231:1274	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	4	31	theme	resulting	892:900	arg1	coefficients					914:925	the resulting significant coefficients	888:925	the resulting significant coefficients	888:925	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	2	32	theme	gum	298:300	arg1	gels					302:305	Gellan gum gels	291:305	Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3	291:412	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	5	33	theme	intended	1001:1008	arg1	property					1021:1028	the intended relaxation property	997:1028	the intended relaxation property from the concentration of the contrast agent	997:1073	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	5	34	theme	R2	1170:1171	arg1	rates					1185:1189	transverse (R2) relaxation rates	1158:1189	transverse (R2) relaxation rates	1158:1189	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	6	35	theme	controllable	1315:1326	arg1	properties					1339:1348	controllable relaxation properties	1315:1348	controllable relaxation properties	1315:1348	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	4	36	theme	significant	902:912	arg1	coefficients					914:925	the resulting significant coefficients	888:925	the resulting significant coefficients	888:925	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	2	37	theme	Gellan	291:296	arg1	gels					302:305	Gellan gum gels	291:305	Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3	291:412	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	38	dep	MATERIALS	269:277	arg1	scanned					432:438	scanned	432:438	scanned from 230 μT to 3 T. Nineteen gel samples	432:479	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	38	dep	MATERIALS	269:277	arg1	prepared					419:426	prepared	419:426	prepared	419:426	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	0	39	theme	relaxation	35:44	arg1	properties					46:55	tunable relaxation properties	27:55	tunable relaxation properties	27:55	Gellan gum-based gels with tunable relaxation properties for MRI phantoms.
30394298	2	40	theme	agents	537:542	arg1	concentrations					510:523	varying concentrations	502:523	varying concentrations of contrast agents	502:542	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	5	41	theme	agent	1069:1073	arg1	concentration					1039:1051	the concentration	1035:1051	the concentration of the contrast agent	1035:1073	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	4	42	theme	RESULTS	796:802	arg1	relaxivity					848:857	RESULTS The model containing SPIONs and metal salts relaxivity	796:857	RESULTS The model containing SPIONs and metal salts relaxivity	796:857	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	2	43	theme	3 T.	455:458	arg1	samples					473:479	3 T. Nineteen gel samples	455:479	3 T. Nineteen gel samples	455:479	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	0	44	theme	tunable	27:33	arg1	properties					46:55	tunable relaxation properties	27:55	tunable relaxation properties	27:55	Gellan gum-based gels with tunable relaxation properties for MRI phantoms.
30394298	5	45	theme	relaxation	1010:1019	arg1	property					1021:1028	the intended relaxation property	997:1028	the intended relaxation property from the concentration of the contrast agent	997:1073	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	1	46	theme	design	200:205	arg1	implementation					180:193	the implementation	176:193	the implementation of a design of experiments model to obtain tunable relaxation properties	176:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	5	47	theme	contrast	1060:1067	arg1	agent					1069:1073	the contrast agent	1056:1073	the contrast agent	1056:1073	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	2	48	theme	quadratic	569:577	arg1	effects					596:602	the linear, quadratic, and interactive effects	557:602	the linear, quadratic, and interactive effects of the contrast agents	557:625	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	5	49	theme	adjusted	1080:1087	arg1	values > 0.97					1092:1104	adjusted R2 values > 0.97	1080:1104	adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates	1080:1189	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	4	50	theme	model	808:812	arg1	relaxivity					848:857	RESULTS The model containing SPIONs and metal salts relaxivity	796:857	RESULTS The model containing SPIONs and metal salts relaxivity	796:857	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	0	51	with	gels	17:20	arg1	properties					46:55	tunable relaxation properties	27:55	tunable relaxation properties	27:55	Gellan gum-based gels with tunable relaxation properties for MRI phantoms.
30394298	2	52	theme	superparamagnetic	336:352	arg1	SPIONs					380:385	SPIONs	380:385	SPIONs	380:385	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	52	theme	superparamagnetic	336:352	arg1	nanoparticles					365:377	newly synthesized superparamagnetic iron oxide nanoparticles	318:377	newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs)	318:386	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	53	theme	contrast	528:535	arg1	agents					537:542	contrast agents	528:542	contrast agents	528:542	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	6	54	theme	relaxation	1328:1337	arg1	properties					1339:1348	controllable relaxation properties	1315:1348	controllable relaxation properties	1315:1348	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	6	55	theme	chemical	1241:1248	arg1	stability					1266:1274	physical, chemical, and biological stability	1231:1274	physical, chemical, and biological stability	1231:1274	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	2	56	theme	synthesized	324:334	arg1	SPIONs					380:385	SPIONs	380:385	SPIONs	380:385	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	2	56	theme	synthesized	324:334	arg1	nanoparticles					365:377	newly synthesized superparamagnetic iron oxide nanoparticles	318:377	newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs)	318:386	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	3	57	theme	methyl	741:746	arg1	methylparaben					767:779	methylparaben	767:779	methylparaben	767:779	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	3	57	theme	methyl	741:746	arg1	4‑hydroxybenzoate					748:764	methyl 4‑hydroxybenzoate	741:764	methyl 4‑hydroxybenzoate (methylparaben)	741:780	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	2	58	theme	linear	561:566	arg1	effects					596:602	the linear, quadratic, and interactive effects	557:602	the linear, quadratic, and interactive effects of the contrast agents	557:625	MATERIALS AND METHODS Gellan gum gels doped with newly synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and either MnCl2 or GdCl3 were prepared and scanned from 230 μT to 3 T. Nineteen gel samples were formulated with varying concentrations of contrast agents to determine the linear, quadratic, and interactive effects of the contrast agents by a central composite design of experiment.
30394298	6	59	dep	CONCLUSION	1192:1201	arg1	contained					1305:1313	contained	1305:1313	contained controllable relaxation properties while maintaining optical clarity	1305:1382	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	6	59	dep	CONCLUSION	1192:1201	arg1	maintained					1220:1229	maintained	1220:1229	maintained physical, chemical, and biological stability for at least four months	1220:1299	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	1	60	theme	gellan	119:124	arg1	gels					136:139	gellan gum-based gels	119:139	gellan gum-based gels	119:139	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	6	61	contain	contained	1305:1313	arg2	properties					1339:1348	controllable relaxation properties	1315:1348	controllable relaxation properties	1315:1348	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	6	61	contain	contained	1305:1313	arg1	material					1211:1218	The gel material	1203:1218	The gel material	1203:1218	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	1	62	with	material	162:169	arg1	implementation					180:193	the implementation	176:193	the implementation of a design of experiments model to obtain tunable relaxation properties	176:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	6	63	theme	gel	1207:1209	arg1	material					1211:1218	The gel material	1203:1218	The gel material	1203:1218	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30394298	1	64	theme	gum-based	126:134	arg1	gels					136:139	gellan gum-based gels	119:139	gellan gum-based gels	119:139	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	4	65	contain	containing	814:823	arg2	salts					842:846	metal salts	836:846	metal salts	836:846	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	4	65	contain	containing	814:823	arg2	SPIONs					825:830	SPIONs	825:830	SPIONs	825:830	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	4	65	contain	containing	814:823	arg1	model					808:812	The model	804:812	RESULTS The model containing SPIONs and metal salts relaxivity	796:857	RESULTS The model containing SPIONs and metal salts relaxivity was analyzed with ANOVA, and the resulting significant coefficients were tabulated.
30394298	1	66	theme	experiments	210:220	arg1	design					200:205	a design	198:205	a design of experiments model to obtain tunable relaxation properties	198:266	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	1	67	theme	gels	136:139	arg1	analysis					107:114	the analysis	103:114	the analysis of gellan gum-based gels	103:139	OBJECT The research follows the analysis of gellan gum-based gels as novel MRI phantom material with the implementation of a design of experiments model to obtain tunable relaxation properties.
30394298	5	68	theme	transverse	1158:1167	arg1	rates					1185:1189	transverse (R2) relaxation rates	1158:1189	transverse (R2) relaxation rates	1158:1189	The mathematical model was able to accurately predict the intended relaxation property from the concentration of the contrast agent with adjusted R2 values > 0.97 for longitudinal (R1) relaxation rates and 0.87 for transverse (R2) relaxation rates.
30394298	0	69	theme	MRI	61:63	arg1	phantoms					65:72	MRI phantoms	61:72	MRI phantoms	61:72	Gellan gum-based gels with tunable relaxation properties for MRI phantoms.
30394298	3	70	theme	microbial	683:691	arg1	growth					693:698	microbial growth	683:698	microbial growth in the gels	683:710	To inhibit microbial growth in the gels and to enable long-term use, methyl 4‑hydroxybenzoate (methylparaben) was utilized.
30394298	6	71	theme	biological	1255:1264	arg1	stability					1266:1274	physical, chemical, and biological stability	1231:1274	physical, chemical, and biological stability	1231:1274	CONCLUSION The gel material maintained physical, chemical, and biological stability for at least four months and contained controllable relaxation properties while maintaining optical clarity.
30865976	8	0	from	effect	1421:1426	arg1	microbiota					1446:1455	early-life gut microbiota	1431:1455	early-life gut microbiota	1431:1455	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	0	from	effect	1421:1426	arg1	findings					1458:1465	findings	1458:1465	findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition	1458:1586	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	1	theme	biology	1542:1548	arg1	significance					1554:1565	significance	1554:1565	significance for infant nutrition	1554:1586	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	1	theme	biology	1542:1548	arg1	understanding					1521:1533	our understanding	1517:1533	our understanding of HMO biology	1517:1548	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	3	2	theme	gut	500:502	arg1	microbiota					504:513	infant gut microbiota	493:513	infant gut microbiota	493:513	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	4	3	theme	basic	581:585	arg1	research					587:594	basic research	581:594	basic research	581:594	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	0	4	theme	Physiological	75:87	arg1	Significance					89:100	Their Physiological Significance	69:100	Their Physiological Significance	69:100	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.
30865976	8	5	theme	clinical	1488:1495	arg1	research					1497:1504	further clinical research	1480:1504	further clinical research	1480:1504	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	1	6	theme	milk	161:164	arg1	lactose					172:178	the milk sugar lactose	157:178	the milk sugar lactose	157:178	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	2	7	theme	HMO	244:246	arg1	composition					248:258	The HMO composition	240:258	The HMO composition of breast milk	240:273	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	6	8	theme	control	1238:1244	arg1	formula					1246:1252	a control formula	1236:1252	a control formula	1236:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	8	9	theme	immune	1381:1386	arg1	protection					1388:1397	immune protection	1381:1397	immune protection in part	1381:1405	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	6	10	theme	reduced	1157:1163	arg1	need					1165:1168	reduced need	1157:1168	reduced need for antibiotics during the first year of life compared to feeding a control formula	1157:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	5	11	theme	age-appropriate	963:977	arg1	growth					979:984	age-appropriate growth	963:984	age-appropriate growth	963:984	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	6	12	with	formula	1074:1080	arg1	HMOs					1089:1092	2 HMOs	1087:1092	2 HMOs	1087:1092	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	2	13	theme	milk	270:273	arg1	composition					248:258	The HMO composition	240:258	The HMO composition of breast milk	240:273	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	8	14	from	protection	1388:1397	arg1	part					1402:1405	part	1402:1405	part	1402:1405	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	4	15	theme	research	587:594	arg1	data					596:599	Observational and basic research data	563:599	Observational and basic research data	563:599	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	6	16	theme	reported	1103:1110	arg1	illnesses					1112:1120	reported illnesses	1103:1120	reported illnesses of the lower respiratory tract	1103:1151	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	7	17	theme	early-life	1268:1277	arg1	composition					1290:1300	early-life microbiota composition	1268:1300	early-life microbiota composition	1268:1300	In parallel, early-life microbiota composition shifted towards that of breastfed infants.
30865976	2	18	theme	breast	263:268	arg1	milk					270:273	breast milk	263:273	breast milk	263:273	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	4	19	theme	immunity	684:691	arg1	establishment					633:645	the establishment	629:645	the establishment of early-life microbiota and mucosal immunity	629:691	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	1	20	theme	sugar	166:170	arg1	lactose					172:178	the milk sugar lactose	157:178	the milk sugar lactose	157:178	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	5	21	theme	Clinical	768:775	arg1	trials					790:795	Clinical intervention trials	768:795	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT),	768:928	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	3	22	theme	infectious	527:536	arg1	diarrhea					538:545	infectious diarrhea	527:545	infectious diarrhea	527:545	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	1	23	theme	lactose	172:178	arg1	elongations					142:152	elongations	142:152	elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid	142:237	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	1	23	theme	lactose	172:178	arg1	oligosaccharides					114:129	Human milk oligosaccharides	103:129	Human milk oligosaccharides (HMOs)	103:136	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	0	24	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.	0:101	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.
30865976	5	25	theme	infant	802:807	arg1	formula					809:815	infant formula	802:815	infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT),	802:928	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	6	26	theme	lower	1129:1133	arg1	tract					1147:1151	the lower respiratory tract	1125:1151	the lower respiratory tract	1125:1151	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	3	27	theme	Clinical	398:405	arg1	studies					421:427	Clinical observational studies	398:427	Clinical observational studies with breastfed infant-mother dyads	398:462	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	0	28	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.	0:101	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.
30865976	6	29	theme	life	1211:1214	arg1	year					1203:1206	the first year	1193:1206	the first year of life compared to feeding a control formula	1193:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	4	30	from	infections	756:765	arg1	protection					740:749	protection	740:749	protection from infections	740:765	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	1	31	theme	Human	103:107	arg1	HMOs					132:135	HMOs	132:135	HMOs	132:135	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	1	31	theme	Human	103:107	arg1	elongations					142:152	elongations	142:152	elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid	142:237	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	1	31	theme	Human	103:107	arg1	oligosaccharides					114:129	Human milk oligosaccharides	103:129	Human milk oligosaccharides (HMOs)	103:136	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	3	32	theme	observational	407:419	arg1	studies					421:427	Clinical observational studies	398:427	Clinical observational studies with breastfed infant-mother dyads	398:462	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	1	33	theme	milk	109:112	arg1	HMOs					132:135	HMOs	132:135	HMOs	132:135	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	1	33	theme	milk	109:112	arg1	elongations					142:152	elongations	142:152	elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid	142:237	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	1	33	theme	milk	109:112	arg1	oligosaccharides					114:129	Human milk oligosaccharides	103:129	Human milk oligosaccharides (HMOs)	103:136	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	6	34	theme	exploratory	1027:1037	arg1	outcomes					1039:1046	exploratory outcomes	1027:1046	exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula	1027:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	4	35	theme	Observational	563:575	arg1	data					596:599	Observational and basic research data	563:599	Observational and basic research data	563:599	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	6	36	theme	tract	1147:1151	arg1	illnesses					1112:1120	reported illnesses	1103:1120	reported illnesses of the lower respiratory tract	1103:1151	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	6	37	theme	infant	1067:1072	arg1	formula					1074:1080	an infant formula	1064:1080	an infant formula with 2 HMOs	1064:1092	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	2	38	theme	lactation	387:395	arg1	stage					378:382	the stage	374:382	the stage of lactation	374:395	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	3	39	theme	infant	493:498	arg1	microbiota					504:513	infant gut microbiota	493:513	infant gut microbiota	493:513	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	4	40	theme	early-life	650:659	arg1	microbiota					661:670	early-life microbiota	650:670	early-life microbiota	650:670	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	4	41	theme	mucosal	676:682	arg1	immunity					684:691	mucosal immunity	676:691	mucosal immunity	676:691	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	3	42	theme	breastfed	434:442	arg1	dyads					458:462	breastfed infant-mother dyads	434:462	breastfed infant-mother dyads	434:462	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	6	43	theme	related	1048:1054	arg1	outcomes					1039:1046	exploratory outcomes	1027:1046	exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula	1027:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	8	44	theme	early-life	1431:1440	arg1	microbiota					1446:1455	early-life gut microbiota	1431:1455	early-life gut microbiota	1431:1455	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	44	theme	early-life	1431:1440	arg1	findings					1458:1465	findings	1458:1465	findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition	1458:1586	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	3	45	theme	infant-mother	444:456	arg1	dyads					458:462	breastfed infant-mother dyads	434:462	breastfed infant-mother dyads	434:462	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	6	46	theme	first	1197:1201	arg1	year					1203:1206	the first year	1193:1206	the first year of life compared to feeding a control formula	1193:1252	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	2	47	theme	fucosyltransferases	331:349	arg1	polymorphisms					301:313	polymorphisms	301:313	polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3	301:364	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	8	48	theme	further	1480:1486	arg1	research					1497:1504	further clinical research	1480:1504	further clinical research	1480:1504	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	49	theme	infant	1571:1576	arg1	nutrition					1578:1586	infant nutrition	1571:1586	infant nutrition	1571:1586	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	50	theme	HMO	1538:1540	arg1	biology					1542:1548	HMO biology	1538:1548	HMO biology	1538:1548	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	2	51	theme	maternal	322:329	arg1	FUT2					352:355	FUT2	352:355	FUT2	352:355	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	2	51	theme	maternal	322:329	arg1	fucosyltransferases					331:349	the maternal fucosyltransferases	318:349	the maternal fucosyltransferases	318:349	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	2	51	theme	maternal	322:329	arg1	FUT3					361:364	FUT3	361:364	FUT3	361:364	The HMO composition of breast milk is strongly influenced by polymorphisms of the maternal fucosyltransferases, FUT2 and FUT3, and by the stage of lactation.
30865976	6	52	dep	fewer	1097:1101	arg1	illnesses					1112:1120	reported illnesses	1103:1120	reported illnesses of the lower respiratory tract	1103:1151	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	3	53	with	studies	421:427	arg1	dyads					458:462	breastfed infant-mother dyads	434:462	breastfed infant-mother dyads	434:462	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	8	54	theme	gut	1442:1444	arg1	microbiota					1446:1455	early-life gut microbiota	1431:1455	early-life gut microbiota	1431:1455	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	8	54	theme	gut	1442:1444	arg1	findings					1458:1465	findings	1458:1465	findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition	1458:1586	Together, HMOs likely contribute to immune protection in part through their effect on early-life gut microbiota, findings that warrant further clinical research to improve our understanding of HMO biology and significance for infant nutrition.
30865976	7	55	theme	breastfed	1326:1334	arg1	infants					1336:1342	breastfed infants	1326:1342	breastfed infants	1326:1342	In parallel, early-life microbiota composition shifted towards that of breastfed infants.
30865976	3	56	theme	specific	474:481	arg1	HMOs					483:486	specific HMOs	474:486	specific HMOs	474:486	Clinical observational studies with breastfed infant-mother dyads associate specific HMOs with infant gut microbiota, morbidity, infectious diarrhea, and allergies.
30865976	6	57	theme	respiratory	1135:1145	arg1	tract					1147:1151	the lower respiratory tract	1125:1151	the lower respiratory tract	1125:1151	A priori defined exploratory outcomes related feeding an infant formula with 2 HMOs to fewer reported illnesses of the lower respiratory tract and reduced need for antibiotics during the first year of life compared to feeding a control formula.
30865976	0	58	dep	Oligosaccharides	11:26	arg1	Significance					89:100	Their Physiological Significance	69:100	Their Physiological Significance	69:100	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.
30865976	0	58	dep	Oligosaccharides	11:26	arg1	Factors					29:35	Factors	29:35	Factors Affecting Their Composition	29:63	Human Milk Oligosaccharides: Factors Affecting Their Composition and Their Physiological Significance.
30865976	7	59	theme	microbiota	1279:1288	arg1	composition					1290:1300	early-life microbiota composition	1268:1300	early-life microbiota composition	1268:1300	In parallel, early-life microbiota composition shifted towards that of breastfed infants.
30865976	5	60	theme	intervention	777:788	arg1	trials					790:795	Clinical intervention trials	768:795	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT),	768:928	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	4	61	theme	microbiota	661:670	arg1	establishment					633:645	the establishment	629:645	the establishment of early-life microbiota and mucosal immunity	629:691	Observational and basic research data suggest that HMOs influence the establishment of early-life microbiota and mucosal immunity and inhibit pathogens, thereby contributing to protection from infections.
30865976	1	62	theme	sialic	227:232	arg1	acid					234:237	sialic acid	227:237	sialic acid	227:237	Human milk oligosaccharides (HMOs) are elongations of the milk sugar lactose by galactose, N-acetylglucosamine, fucose; and sialic acid.
30865976	5	63	with	trials	790:795	arg1	formula					809:815	infant formula	802:815	infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT),	802:928	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	5	64	theme	single	839:844	arg1	HMO					846:848	the single HMO	835:848	the single HMO	835:848	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30865976	5	64	theme	single	839:844	arg1	2'-fucosyllactose					851:867	2'-fucosyllactose	851:867	2'-fucosyllactose (2'FL)	851:874	Clinical intervention trials with infant formula supplemented with the single HMO, 2'-fucosyllactose (2'FL), or with 2 HMOs, 2'FL and lacto-N-neotetraose (LNnT), demonstrated that they allow for age-appropriate growth and are well tolerated.
30287045	10	0	theme	osteogenic	1710:1719	arg1	expression					1728:1737	osteogenic marker expression	1710:1737	osteogenic marker expression	1710:1737	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	6	1	theme	gelation	966:973	arg1	technique					975:983	internal gelation technique	957:983	internal gelation technique with gas foaming	957:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	7	2	theme	osteogenic	1124:1133	arg1	differentiation					1135:1149	the osteogenic differentiation	1120:1149	the osteogenic differentiation	1120:1149	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	9	3	theme	gelation	1349:1356	arg1	technique					1358:1366	internal gelation technique	1340:1366	internal gelation technique with gas foaming	1340:1383	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	1	4	theme	bone	147:150	arg1	defects					152:158	repair and regenerate bone defects	125:158	repair and regenerate bone defects in the field of biomedical applications	125:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	10	5	theme	biological	1564:1573	arg1	assays					1575:1580	biological assays	1564:1580	biological assays	1564:1580	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	5	6	theme	osteogenic	793:802	arg1	differentiation					804:818	osteogenic differentiation	793:818	osteogenic differentiation	793:818	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	6	7	theme	MAFs	912:915	arg1	fabrication					917:927	MAFs fabrication	912:927	MAFs fabrication based on the combination of internal gelation technique with gas foaming	912:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	5	8	theme	macroporous	683:693	arg1	MAFs					711:714	MAFs	711:714	MAFs	711:714	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	8	theme	macroporous	683:693	arg1	foams					704:708	macroporous alginate foams	683:708	macroporous alginate foams (MAFs)	683:715	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	10	9	theme	marker	1721:1726	arg1	expression					1728:1737	osteogenic marker expression	1710:1737	osteogenic marker expression	1710:1737	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	7	10	with	combination	1029:1039	arg1	calcium					1046:1052	calcium	1046:1052	calcium	1046:1052	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	4	11	theme	surface	591:597	arg1	interaction					599:609	cell-material surface interaction	577:609	cell-material surface interaction	577:609	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	3	12	theme	suitable	370:377	arg1	properties					359:368	biophysical properties	347:368	biophysical properties suitable to address biological response towards newly bone tissue formation	347:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	4	13	theme	cell	517:520	arg1	migration					522:530	cell migration	517:530	cell migration	517:530	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	5	14	theme	alginate	695:702	arg1	MAFs					711:714	MAFs	711:714	MAFs	711:714	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	14	theme	alginate	695:702	arg1	foams					704:708	macroporous alginate foams	683:708	macroporous alginate foams (MAFs)	683:715	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	1	15	from	defects	152:158	arg1	field					167:171	the field	163:171	the field of biomedical applications	163:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	2	16	theme	bone	292:295	arg1	tissue					297:302	bone tissue	292:302	bone tissue	292:302	Novel approaches include the design of natural bioactive scaffolds mimicking bone tissue.
30287045	10	17	theme	cell	1651:1654	arg1	growth					1656:1661	cell growth	1651:1661	cell growth	1651:1661	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	4	18	theme	osteogenic	615:624	arg1	transmission					634:645	osteogenic signals transmission	615:645	osteogenic signals transmission	615:645	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	8	19	theme	strontium	1169:1177	arg1	ions					1179:1182	strontium ions	1169:1182	strontium ions	1169:1182	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	10	20	from	growth	1656:1661	arg1	times					1691:1695	long times	1686:1695	long times	1686:1695	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	5	21	theme	foams	704:708	arg1	development					668:678	the development	664:678	the development of macroporous alginate foams (MAFs)	664:715	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	10	22	with	scaffolds	1593:1601	arg1	content					1623:1629	high strontium content	1608:1629	high strontium content	1608:1629	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	9	23	dep	able	1520:1523	arg1	combination					1325:1335	the combination	1321:1335	the combination of internal gelation technique with gas foaming followed by freeze-drying	1321:1409	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	23	dep	able	1520:1523	arg1	procedure					1442:1450	an easy and straightforward procedure	1414:1450	an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity	1414:1517	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	23	dep	able	1520:1523	arg1	able					1520:1523	able	1520:1523	able	1520:1523	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	6	24	theme	gas	990:992	arg1	foaming					994:1000	gas foaming	990:1000	gas foaming	990:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	3	25	theme	biophysical	347:357	arg1	properties					359:368	biophysical properties	347:368	biophysical properties suitable to address biological response towards newly bone tissue formation	347:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	6	26	theme	new	897:899	arg1	method					901:906	a new method	895:906	a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming	895:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	5	27	theme	Stem	841:844	arg1	Cells					846:850	human Mesenchymal Stem Cells	823:850	human Mesenchymal Stem Cells (hMSCs)	823:858	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	27	theme	Stem	841:844	arg1	hMSCs					853:857	hMSCs	853:857	hMSCs	853:857	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	2	28	theme	scaffolds	272:280	arg1	design					244:249	the design	240:249	the design of natural bioactive scaffolds mimicking bone tissue	240:302	Novel approaches include the design of natural bioactive scaffolds mimicking bone tissue.
30287045	9	29	theme	high	1483:1486	arg1	porosity					1488:1495	high porosity	1483:1495	high porosity	1483:1495	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	5	30	theme	Mesenchymal	829:839	arg1	Cells					846:850	human Mesenchymal Stem Cells	823:850	human Mesenchymal Stem Cells (hMSCs)	823:858	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	30	theme	Mesenchymal	829:839	arg1	hMSCs					853:857	hMSCs	853:857	hMSCs	853:857	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	4	31	theme	pivotal	501:507	arg1	role					509:512	a pivotal role	499:512	a pivotal role	499:512	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	2	32	theme	bioactive	262:270	arg1	scaffolds					272:280	natural bioactive scaffolds	254:280	natural bioactive scaffolds mimicking bone tissue	254:302	Novel approaches include the design of natural bioactive scaffolds mimicking bone tissue.
30287045	8	33	theme	physiological	1249:1261	arg1	medium					1263:1268	physiological medium	1249:1268	physiological medium of MAFs	1249:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	0	34	theme	alginate	12:19	arg1	foams					21:25	Macroporous alginate foams	0:25	Macroporous alginate foams	0:25	Macroporous alginate foams crosslinked with strontium for bone tissue engineering.
30287045	8	35	from	properties	1219:1228	arg1	medium					1263:1268	physiological medium	1249:1268	physiological medium of MAFs	1249:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	8	36	from	degradation	1234:1244	arg1	medium					1263:1268	physiological medium	1249:1268	physiological medium of MAFs	1249:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	7	37	theme	cross-linking	1057:1069	arg1	Strontium					1003:1011	Strontium	1003:1011	Strontium	1003:1011	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	7	37	theme	cross-linking	1057:1069	arg1	agent					1071:1075	cross-linking agent	1057:1075	cross-linking agent for the alginate chains	1057:1099	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	8	38	theme	MAFs	1273:1276	arg1	medium					1263:1268	physiological medium	1249:1268	physiological medium of MAFs	1249:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	0	39	theme	Macroporous	0:10	arg1	foams					21:25	Macroporous alginate foams	0:25	Macroporous alginate foams	0:25	Macroporous alginate foams crosslinked with strontium for bone tissue engineering.
30287045	9	40	theme	technique	1358:1366	arg1	procedure					1442:1450	an easy and straightforward procedure	1414:1450	an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity	1414:1517	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	40	theme	technique	1358:1366	arg1	combination					1325:1335	the combination	1321:1335	the combination of internal gelation technique with gas foaming followed by freeze-drying	1321:1409	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	40	theme	technique	1358:1366	arg1	able					1520:1523	able	1520:1523	able	1520:1523	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	41	with	technique	1358:1366	arg1	foaming					1377:1383	gas foaming	1373:1383	gas foaming	1373:1383	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	7	42	theme	differentiation	1135:1149	arg1	enhancer					1108:1115	enhancer	1108:1115	enhancer of the osteogenic differentiation	1108:1149	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	7	42	theme	differentiation	1135:1149	arg1	Strontium					1003:1011	Strontium	1003:1011	Strontium	1003:1011	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	8	43	theme	physical	1210:1217	arg1	properties					1219:1228	physical properties	1210:1228	physical properties	1210:1228	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	5	44	theme	Cells	846:850	arg1	growth					782:787	growth	782:787	growth	782:787	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	44	theme	Cells	846:850	arg1	differentiation					804:818	osteogenic differentiation	793:818	osteogenic differentiation	793:818	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	45	theme	porous	722:727	arg1	structure					753:761	porous and well interconnected structure	722:761	porous and well interconnected structure	722:761	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	10	46	theme	long	1686:1689	arg1	times					1691:1695	long times	1686:1695	long times	1686:1695	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	5	47	theme	human	823:827	arg1	Cells					846:850	human Mesenchymal Stem Cells	823:850	human Mesenchymal Stem Cells (hMSCs)	823:858	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	5	47	theme	human	823:827	arg1	hMSCs					853:857	hMSCs	853:857	hMSCs	853:857	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	10	48	from	differentiation	1667:1681	arg1	times					1691:1695	long times	1686:1695	long times	1686:1695	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	1	49	theme	biomedical	176:185	arg1	applications					187:198	biomedical applications	176:198	biomedical applications	176:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	3	50	theme	biological	390:399	arg1	response					401:408	biological response	390:408	biological response towards newly bone tissue formation	390:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	4	51	theme	cell-material	577:589	arg1	interaction					599:609	cell-material surface interaction	577:609	cell-material surface interaction	577:609	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	7	52	theme	alginate	1085:1092	arg1	chains					1094:1099	the alginate chains	1081:1099	the alginate chains	1081:1099	Strontium was employed in combination with calcium as cross-linking agent for the alginate chains and as enhancer of the osteogenic differentiation.
30287045	9	53	theme	alginate	1463:1470	arg1	foams					1472:1476	alginate foams	1463:1476	alginate foams	1463:1476	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	1	54	theme	applications	187:198	arg1	field					167:171	the field	163:171	the field of biomedical applications	163:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	3	55	theme	tissue	429:434	arg1	formation					436:444	newly bone tissue formation	418:444	newly bone tissue formation	418:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	6	56	theme	internal	957:964	arg1	technique					975:983	internal gelation technique	957:983	internal gelation technique with gas foaming	957:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	4	57	theme	scaffold	462:469	arg1	porosity					471:478	scaffold porosity	462:478	scaffold porosity	462:478	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	9	58	theme	gas	1373:1375	arg1	foaming					1377:1383	gas foaming	1373:1383	gas foaming	1373:1383	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	5	59	theme	interconnected	738:751	arg1	structure					753:761	porous and well interconnected structure	722:761	porous and well interconnected structure	722:761	Here we propose the development of macroporous alginate foams (MAFs) with porous and well interconnected structure, useful to enhance growth and osteogenic differentiation of human Mesenchymal Stem Cells (hMSCs).
30287045	4	60	theme	pore	484:487	arg1	size					489:492	pore size	484:492	pore size	484:492	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	0	61	theme	bone	58:61	arg1	engineering					70:80	bone tissue engineering	58:80	bone tissue engineering	58:80	Macroporous alginate foams crosslinked with strontium for bone tissue engineering.
30287045	8	62	theme	ions	1179:1182	arg1	influence					1156:1164	The influence	1152:1164	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs	1152:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	10	63	theme	strontium	1613:1621	arg1	content					1623:1629	high strontium content	1608:1629	high strontium content	1608:1629	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	6	64	theme	technique	975:983	arg1	combination					942:952	the combination	938:952	the combination of internal gelation technique with gas foaming	938:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	2	65	theme	Novel	215:219	arg1	approaches					221:230	Novel approaches	215:230	Novel approaches	215:230	Novel approaches include the design of natural bioactive scaffolds mimicking bone tissue.
30287045	9	66	theme	internal	1340:1347	arg1	technique					1358:1366	internal gelation technique	1340:1366	internal gelation technique with gas foaming	1340:1383	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	1	67	theme	novel	105:109	arg1	strategies					111:120	novel strategies	105:120	novel strategies	105:120	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	3	68	theme	bone	424:427	arg1	formation					436:444	newly bone tissue formation	418:444	newly bone tissue formation	418:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	3	69	theme	bioactive	311:319	arg1	scaffolds					321:329	These bioactive scaffolds	305:329	These bioactive scaffolds	305:329	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	8	70	from	kinetics	1200:1207	arg1	medium					1263:1268	physiological medium	1249:1268	physiological medium of MAFs	1249:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	1	71	theme	strategies	111:120	arg1	Nowadays					83:90	Nowadays	83:90	Nowadays	83:90	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	1	71	theme	strategies	111:120	arg1	need					97:100	the need	93:100	the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications	93:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	2	72	theme	natural	254:260	arg1	scaffolds					272:280	natural bioactive scaffolds	254:280	natural bioactive scaffolds mimicking bone tissue	254:302	Novel approaches include the design of natural bioactive scaffolds mimicking bone tissue.
30287045	9	73	theme	cell	1536:1539	arg1	infiltration					1541:1552	cell infiltration	1536:1552	cell infiltration	1536:1552	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	6	74	with	technique	975:983	arg1	foaming					994:1000	gas foaming	990:1000	gas foaming	990:1000	Moreover, in this study we report a new method for MAFs fabrication based on the combination of internal gelation technique with gas foaming.
30287045	9	75	theme	easy	1417:1420	arg1	combination					1325:1335	the combination	1321:1335	the combination of internal gelation technique with gas foaming followed by freeze-drying	1321:1409	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	75	theme	easy	1417:1420	arg1	procedure					1442:1450	an easy and straightforward procedure	1414:1450	an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity	1414:1517	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	75	theme	easy	1417:1420	arg1	able					1520:1523	able	1520:1523	able	1520:1523	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	8	76	theme	gelation	1191:1198	arg1	kinetics					1200:1207	the gelation kinetics	1187:1207	the gelation kinetics	1187:1207	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	1	77	theme	repair	125:130	arg1	defects					152:158	repair and regenerate bone defects	125:158	repair and regenerate bone defects in the field of biomedical applications	125:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	4	78	theme	signals	626:632	arg1	transmission					634:645	osteogenic signals transmission	615:645	osteogenic signals transmission	615:645	In particular, scaffold porosity and pore size play a pivotal role in cell migration, adhesion and proliferation, thus increasing cell-material surface interaction and osteogenic signals transmission.
30287045	8	79	from	influence	1156:1164	arg1	degradation					1234:1244	degradation	1234:1244	degradation in physiological medium of MAFs	1234:1276	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	8	79	from	influence	1156:1164	arg1	kinetics					1200:1207	the gelation kinetics	1187:1207	the gelation kinetics	1187:1207	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	8	79	from	influence	1156:1164	arg1	properties					1219:1228	physical properties	1210:1228	physical properties	1210:1228	The influence of strontium ions on the gelation kinetics, physical properties and degradation in physiological medium of MAFs was investigated.
30287045	10	80	theme	high	1608:1611	arg1	content					1623:1629	high strontium content	1608:1629	high strontium content	1608:1629	Finally, biological assays showed how scaffolds with high strontium content are able to support cell growth and differentiation in long times by promoting osteogenic marker expression.
30287045	0	81	theme	tissue	63:68	arg1	engineering					70:80	bone tissue engineering	58:80	bone tissue engineering	58:80	Macroporous alginate foams crosslinked with strontium for bone tissue engineering.
30287045	3	82	contain	possess	339:345	arg1	scaffolds					321:329	These bioactive scaffolds	305:329	These bioactive scaffolds	305:329	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	3	82	contain	possess	339:345	arg2	properties					359:368	biophysical properties	347:368	biophysical properties suitable to address biological response towards newly bone tissue formation	347:444	These bioactive scaffolds have to possess biophysical properties suitable to address biological response towards newly bone tissue formation.
30287045	1	83	theme	regenerate	136:145	arg1	defects					152:158	repair and regenerate bone defects	125:158	repair and regenerate bone defects in the field of biomedical applications	125:198	Nowadays, the need of novel strategies to repair and regenerate bone defects in the field of biomedical applications has increased.
30287045	9	84	theme	straightforward	1426:1440	arg1	combination					1325:1335	the combination	1321:1335	the combination of internal gelation technique with gas foaming followed by freeze-drying	1321:1409	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	84	theme	straightforward	1426:1440	arg1	procedure					1442:1450	an easy and straightforward procedure	1414:1450	an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity	1414:1517	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30287045	9	84	theme	straightforward	1426:1440	arg1	able					1520:1523	able	1520:1523	able	1520:1523	Our results suggest that the combination of internal gelation technique with gas foaming followed by freeze-drying is an easy and straightforward procedure to prepare alginate foams with high porosity and interconnectivity, able to support cell infiltration.
30797008	5	0	with	CH	957:958	arg1	homogenisation					976:989	ultrasonic homogenisation	965:989	ultrasonic homogenisation	965:989	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	11	1	theme	Cell	1563:1566	arg1	results					1578:1584	Cell viability results	1563:1584	Cell viability results	1563:1584	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	9	2	theme	cell	1434:1437	arg1	line					1439:1442	SaOS-2 cell line	1427:1442	SaOS-2 cell line	1427:1442	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	3	3	theme	tissue	687:692	arg1	regeneration					694:705	bone tissue regeneration	682:705	bone tissue regeneration	682:705	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	11	4	theme	control	1677:1683	arg1	group					1685:1689	control group	1677:1689	control group	1677:1689	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	2	5	with	material	404:411	arg1	similarity					433:442	its structural similarity	418:442	its structural similarity to bone tissue due to including type I collagen and hydroxyapatite	418:509	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	3	6	theme	present	566:572	arg1	study					574:578	the present study	562:578	the present study	562:578	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	12	7	theme	osteogenic	1791:1800	arg1	activity					1802:1809	osteogenic activity	1791:1809	osteogenic activity	1791:1809	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	8	8	theme	degradation	1271:1281	arg1	rate					1283:1286	degradation rate	1271:1286	degradation rate	1271:1286	Mechanical properties and degradation rate are enhanced with increasing FS content.
30797008	3	9	with	scaffold	621:628	arg1	incorporation					638:650	FS incorporation	635:650	FS incorporation into chitosan (CH) matrix	635:676	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	1	10	theme	scaffold	211:218	arg1	design					220:225	scaffold design	211:225	scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	211:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	2	11	theme	distinctive	525:535	arg1	arrangement					546:556	distinctive collagen arrangement	525:556	distinctive collagen arrangement	525:556	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	0	12	theme	incorporated	21:32	arg1	scaffolds					56:64	incorporated chitosan biocomposite scaffolds	21:64	incorporated chitosan biocomposite scaffolds for bone tissue engineering	21:92	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	11	13	contain	had	1617:1619	arg2	effect					1637:1642	a proliferative effect	1621:1642	a proliferative effect	1621:1642	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	11	13	contain	had	1617:1619	arg1	microparticles					1602:1615	FS microparticles	1599:1615	FS microparticles	1599:1615	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	8	14	theme	FS	1317:1318	arg1	content					1320:1326	FS content	1317:1326	FS content	1317:1326	Mechanical properties and degradation rate are enhanced with increasing FS content.
30797008	13	15	theme	polymeric	1983:1991	arg1	scaffolds					1993:2001	polymeric scaffolds	1983:2001	polymeric scaffolds	1983:2001	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	12	16	theme	microparticle	1753:1765	arg1	reinforcement					1767:1779	FS microparticle reinforcement	1750:1779	FS microparticle reinforcement	1750:1779	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	0	17	theme	bone	70:73	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	2	18	theme	type	476:479	arg1	collagen					483:490	including type I collagen	466:490	including type I collagen	466:490	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	7	19	theme	uniform	1199:1205	arg1	structure					1212:1220	uniform pore structure	1199:1220	uniform pore structure showing high porosity	1199:1242	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	4	20	theme	fish	750:753	arg1	macromolecules					734:747	two biological macromolecules	719:747	two biological macromolecules	719:747	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	4	20	theme	fish	750:753	arg1	scale					755:759	fish scale	750:759	fish scale	750:759	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	9	21	theme	In	1329:1330	arg1	cytotoxicity					1338:1349	In vitro cytotoxicity	1329:1349	In vitro cytotoxicity	1329:1349	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	0	22	theme	biocomposite	43:54	arg1	scaffolds					56:64	incorporated chitosan biocomposite scaffolds	21:64	incorporated chitosan biocomposite scaffolds for bone tissue engineering	21:92	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	12	23	theme	ALP	1692:1694	arg1	activity					1696:1703	ALP activity	1692:1703	ALP activity	1692:1703	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	13	24	theme	promising	1937:1945	arg1	effects					1947:1953	promising effects	1937:1953	promising effects	1937:1953	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	24	theme	promising	1937:1945	arg1	macromolecule					1850:1862	a biological macromolecule	1837:1862	a biological macromolecule with unique characteristics	1837:1890	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	24	theme	promising	1937:1945	arg1	FS					1893:1894	FS	1893:1894	FS	1893:1894	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	24	theme	promising	1937:1945	arg1	agents					1972:1977	reinforcement agents	1958:1977	reinforcement agents for polymeric scaffolds	1958:2001	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	0	25	theme	Bioactive	0:8	arg1	fish					10:13	Bioactive fish	0:13	Bioactive fish	0:13	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	1	26	theme	human	283:287	arg1	structure					296:304	the human tissue structure	279:304	the human tissue structure with their physical and chemical similarity	279:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	14	27	theme	tissue	2077:2082	arg1	applications					2096:2107	bone tissue engineering applications	2072:2107	bone tissue engineering applications	2072:2107	In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications.
30797008	2	28	theme	structural	422:431	arg1	similarity					433:442	its structural similarity	418:442	its structural similarity to bone tissue due to including type I collagen and hydroxyapatite	418:509	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	1	29	theme	active	118:123	arg1	materials					198:206	potential materials	188:206	potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	188:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	1	29	theme	active	118:123	arg1	macromolecules					133:146	biologically active natural macromolecules	105:146	biologically active natural macromolecules	105:146	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	2	30	theme	active	397:402	arg1	scale					368:372	fish scale	363:372	fish scale (FS)	363:377	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	30	theme	active	397:402	arg1	material					404:411	a biologically active material	382:411	a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite	382:509	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	0	31	theme	chitosan	34:41	arg1	scaffolds					56:64	incorporated chitosan biocomposite scaffolds	21:64	incorporated chitosan biocomposite scaffolds for bone tissue engineering	21:92	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	9	32	theme	osteogenic	1370:1379	arg1	activity					1381:1388	osteogenic activity	1370:1388	osteogenic activity	1370:1388	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	10	33	theme	culture	1547:1553	arg1	period					1555:1560	culture period	1547:1560	culture period	1547:1560	CH/FS scaffolds did not show any cytotoxicity effect and the cells were gradually proliferated during culture period.
30797008	3	34	theme	composite	611:619	arg1	scaffold					621:628	a novel composite scaffold	603:628	a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration	603:705	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	14	35	theme	CH/FS	2030:2034	arg1	bio-composites					2036:2049	fabricated CH/FS bio-composites	2019:2049	fabricated CH/FS bio-composites	2019:2049	In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications.
30797008	3	36	theme	chitosan	657:664	arg1	matrix					671:676	chitosan (CH) matrix	657:676	chitosan (CH) matrix	657:676	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	7	37	contain	have	1194:1197	arg2	structure					1212:1220	uniform pore structure	1199:1220	uniform pore structure showing high porosity	1199:1242	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	7	37	contain	have	1194:1197	arg1	scaffolds					1184:1192	CH/FS scaffolds	1178:1192	CH/FS scaffolds	1178:1192	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	9	38	theme	scaffolds	1397:1405	arg1	proliferation					1352:1364	proliferation	1352:1364	proliferation	1352:1364	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	9	38	theme	scaffolds	1397:1405	arg1	activity					1381:1388	osteogenic activity	1370:1388	osteogenic activity	1370:1388	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	9	38	theme	scaffolds	1397:1405	arg1	cytotoxicity					1338:1349	In vitro cytotoxicity	1329:1349	In vitro cytotoxicity	1329:1349	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	11	39	theme	SaOS-2	1647:1652	arg1	cells					1654:1658	SaOS-2 cells	1647:1658	SaOS-2 cells	1647:1658	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	1	40	theme	unique	240:245	arg1	characteristics					247:261	their unique characteristics	234:261	their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	234:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	5	41	theme	ultrasonic	965:974	arg1	homogenisation					976:989	ultrasonic homogenisation	965:989	ultrasonic homogenisation	965:989	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	2	42	theme	fish	363:366	arg1	material					404:411	a biologically active material	382:411	a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite	382:509	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	42	theme	fish	363:366	arg1	FS					375:376	FS	375:376	FS	375:376	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	42	theme	fish	363:366	arg1	scale					368:372	fish scale	363:372	fish scale (FS)	363:377	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	5	43	from	dispersal	944:952	arg1	CH					957:958	CH	957:958	CH with ultrasonic homogenisation	957:989	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	9	44	theme	SaOS-2	1427:1432	arg1	line					1439:1442	SaOS-2 cell line	1427:1442	SaOS-2 cell line	1427:1442	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	12	45	theme	culture	1818:1824	arg1	period					1826:1831	culture period	1818:1831	culture period	1818:1831	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	11	46	theme	viability	1568:1576	arg1	results					1578:1584	Cell viability results	1563:1584	Cell viability results	1563:1584	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	13	47	theme	biological	1839:1848	arg1	effects					1947:1953	promising effects	1937:1953	promising effects	1937:1953	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	47	theme	biological	1839:1848	arg1	macromolecule					1850:1862	a biological macromolecule	1837:1862	a biological macromolecule with unique characteristics	1837:1890	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	47	theme	biological	1839:1848	arg1	FS					1893:1894	FS	1893:1894	FS	1893:1894	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	4	48	theme	bio-composite	800:812	arg1	scaffold					814:821	bio-composite scaffold	800:821	bio-composite scaffold	800:821	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	1	49	with	structure	296:304	arg1	similarity					339:348	their physical and chemical similarity	311:348	their physical and chemical similarity	311:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	3	50	theme	bone	682:685	arg1	regeneration					694:705	bone tissue regeneration	682:705	bone tissue regeneration	682:705	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	1	51	theme	potential	188:196	arg1	materials					198:206	potential materials	188:206	potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	188:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	1	51	theme	potential	188:196	arg1	macromolecules					133:146	biologically active natural macromolecules	105:146	biologically active natural macromolecules	105:146	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	8	52	theme	Mechanical	1245:1254	arg1	properties					1256:1265	Mechanical properties	1245:1265	Mechanical properties	1245:1265	Mechanical properties and degradation rate are enhanced with increasing FS content.
30797008	2	53	theme	collagen	537:544	arg1	arrangement					546:556	distinctive collagen arrangement	525:556	distinctive collagen arrangement	525:556	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	1	54	theme	natural	125:131	arg1	materials					198:206	potential materials	188:206	potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	188:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	1	54	theme	natural	125:131	arg1	macromolecules					133:146	biologically active natural macromolecules	105:146	biologically active natural macromolecules	105:146	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	0	55	theme	tissue	75:80	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering.
30797008	5	56	theme	lyophilization	1027:1040	arg1	technique					1042:1050	lyophilization technique	1027:1050	lyophilization technique	1027:1050	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	1	57	from	materials	198:206	arg1	design					220:225	scaffold design	211:225	scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	211:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	12	58	theme	FS	1750:1751	arg1	reinforcement					1767:1779	FS microparticle reinforcement	1750:1779	FS microparticle reinforcement	1750:1779	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	2	59	theme	I	481:481	arg1	collagen					483:490	including type I collagen	466:490	including type I collagen	466:490	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	1	60	used	used	180:183	arg2	materials					198:206	potential materials	188:206	potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity	188:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	1	60	used	used	180:183	arg2	macromolecules					133:146	biologically active natural macromolecules	105:146	biologically active natural macromolecules	105:146	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	7	61	theme	pore	1207:1210	arg1	structure					1212:1220	uniform pore structure	1199:1220	uniform pore structure showing high porosity	1199:1242	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	5	62	theme	chemical	863:870	arg1	method					872:877	the chemical method	859:877	the chemical method	859:877	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	2	63	theme	including	466:474	arg1	collagen					483:490	including type I collagen	466:490	including type I collagen	466:490	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	13	64	theme	reinforcement	1958:1970	arg1	effects					1947:1953	promising effects	1937:1953	promising effects	1937:1953	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	13	64	theme	reinforcement	1958:1970	arg1	agents					1972:1977	reinforcement agents	1958:1977	reinforcement agents for polymeric scaffolds	1958:2001	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	12	65	theme	biomineralization	1709:1725	arg1	studies					1727:1733	biomineralization studies	1709:1733	biomineralization studies	1709:1733	ALP activity and biomineralization studies indicated that FS microparticle reinforcement increased osteogenic activity during culture period.
30797008	10	66	theme	CH/FS	1445:1449	arg1	scaffolds					1451:1459	CH/FS scaffolds	1445:1459	CH/FS scaffolds	1445:1459	CH/FS scaffolds did not show any cytotoxicity effect and the cells were gradually proliferated during culture period.
30797008	4	67	theme	biological	723:732	arg1	chitosan					765:772	chitosan	765:772	chitosan	765:772	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	4	67	theme	biological	723:732	arg1	macromolecules					734:747	two biological macromolecules	719:747	two biological macromolecules	719:747	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	4	67	theme	biological	723:732	arg1	scale					755:759	fish scale	750:759	fish scale	750:759	Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold.
30797008	2	68	contain	have	520:523	arg2	arrangement					546:556	distinctive collagen arrangement	525:556	distinctive collagen arrangement	525:556	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	68	contain	have	520:523	arg1	FS					375:376	FS	375:376	FS	375:376	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	68	contain	have	520:523	arg1	scale					368:372	fish scale	363:372	fish scale (FS)	363:377	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	2	68	contain	have	520:523	arg1	material					404:411	a biologically active material	382:411	a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite	382:509	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	7	69	theme	CH/FS	1178:1182	arg1	scaffolds					1184:1192	CH/FS scaffolds	1178:1192	CH/FS scaffolds	1178:1192	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	10	70	theme	cytotoxicity	1478:1489	arg1	effect					1491:1496	any cytotoxicity effect	1474:1496	any cytotoxicity effect	1474:1496	CH/FS scaffolds did not show any cytotoxicity effect and the cells were gradually proliferated during culture period.
30797008	5	71	dep	disrupted	883:891	arg1	followed					932:939	followed	932:939	followed by dispersal in CH with ultrasonic homogenisation	932:989	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	14	72	theme	engineering	2084:2094	arg1	applications					2096:2107	bone tissue engineering applications	2072:2107	bone tissue engineering applications	2072:2107	In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications.
30797008	7	73	theme	porosity	1149:1156	arg1	results					1158:1164	SEM and porosity results	1141:1164	SEM and porosity results	1141:1164	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	1	74	theme	tissue	289:294	arg1	structure					296:304	the human tissue structure	279:304	the human tissue structure with their physical and chemical similarity	279:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	14	75	theme	bone	2072:2075	arg1	applications					2096:2107	bone tissue engineering applications	2072:2107	bone tissue engineering applications	2072:2107	In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications.
30797008	7	76	theme	SEM	1141:1143	arg1	results					1158:1164	SEM and porosity results	1141:1164	SEM and porosity results	1141:1164	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	9	77	dep	In	1329:1330	arg1	vitro					1332:1336	vitro	1332:1336	vitro	1332:1336	In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line.
30797008	3	78	theme	novel	605:609	arg1	scaffold					621:628	a novel composite scaffold	603:628	a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration	603:705	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	13	79	with	macromolecule	1850:1862	arg1	characteristics					1876:1890	unique characteristics	1869:1890	unique characteristics	1869:1890	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	1	80	theme	physical	317:324	arg1	similarity					339:348	their physical and chemical similarity	311:348	their physical and chemical similarity	311:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	5	81	theme	CH/FS	992:996	arg1	scaffolds					998:1006	CH/FS scaffolds	992:1006	CH/FS scaffolds	992:1006	First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 μm), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique.
30797008	2	82	theme	bone	447:450	arg1	tissue					452:457	bone tissue	447:457	bone tissue	447:457	Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement.
30797008	11	83	theme	FS	1599:1600	arg1	microparticles					1602:1615	FS microparticles	1599:1615	FS microparticles	1599:1615	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
30797008	1	84	theme	chemical	330:337	arg1	similarity					339:348	their physical and chemical similarity	311:348	their physical and chemical similarity	311:348	Recently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity.
30797008	14	85	theme	fabricated	2019:2028	arg1	bio-composites					2036:2049	fabricated CH/FS bio-composites	2019:2049	fabricated CH/FS bio-composites	2019:2049	In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications.
30797008	3	86	theme	FS	635:636	arg1	incorporation					638:650	FS incorporation	635:650	FS incorporation into chitosan (CH) matrix	635:676	In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration.
30797008	7	87	theme	high	1230:1233	arg1	porosity					1235:1242	high porosity	1230:1242	high porosity	1230:1242	SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity.
30797008	13	88	theme	unique	1869:1874	arg1	characteristics					1876:1890	unique characteristics	1869:1890	unique characteristics	1869:1890	As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds.
30797008	11	89	theme	proliferative	1623:1635	arg1	effect					1637:1642	a proliferative effect	1621:1642	a proliferative effect	1621:1642	Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group.
29555510	5	0	from	Changes	784:790	arg1	intensity					799:807	the intensity	795:807	the intensity of interactions between the two biopolymers	795:851	Changes in the intensity of interactions between the two biopolymers resulted in films with different properties.
29555510	9	1	theme	composite	1377:1385	arg1	films					1387:1391	composite films	1377:1391	composite films	1377:1391	FTIR spectra of composite films confirmed that gelatin and CMC were not covalently bonded.
29555510	8	2	theme	swelling	1296:1303	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	2	theme	swelling	1296:1303	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	2	theme	swelling	1296:1303	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	2	theme	swelling	1296:1303	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	2	theme	swelling	1296:1303	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	2	theme	swelling	1296:1303	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	1	3	theme	physical	309:316	arg1	properties					333:342	the physical and mechanical properties	305:342	the physical and mechanical properties of gelatin-CMC composite films	305:373	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	8	4	theme	9:1	1159:1161	arg1	MR					1153:1154	MR	1153:1154	MR of 9:1 and pHopt (corresponding to the maximum amount of interaction)	1153:1224	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	4	5	theme	mechanical	669:678	arg1	properties					680:689	Physical and mechanical properties	656:689	Physical and mechanical properties of resultant composite films	656:718	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	3	6	theme	relative	620:627	arg1	viscosity					629:637	the relative viscosity	616:637	the relative viscosity of mixed system	616:653	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	0	7	theme	interactions	103:114	arg1	influence					76:84	the influence	72:84	the influence of electrostatic interactions	72:114	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	6	8	theme	Polymer	898:904	arg1	complexation					906:917	Polymer complexation	898:917	Polymer complexation	898:917	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	7	9	theme	continuous	1099:1108	arg1	films					1118:1122	continuous gelatin films	1099:1122	continuous gelatin films	1099:1122	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	5	10	theme	different	876:884	arg1	properties					886:895	different properties	876:895	different properties	876:895	Changes in the intensity of interactions between the two biopolymers resulted in films with different properties.
29555510	1	11	theme	pH	261:262	arg1	function					249:256	a function	247:256	a function of pH and mixing ratio (MR)	247:284	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	0	12	theme	electrostatic	89:101	arg1	interactions					103:114	electrostatic interactions	89:114	electrostatic interactions	89:114	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	1	13	theme	mechanical	322:331	arg1	properties					333:342	the physical and mechanical properties	305:342	the physical and mechanical properties of gelatin-CMC composite films	305:373	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	7	14	theme	gelatin	1110:1116	arg1	films					1118:1122	continuous gelatin films	1099:1122	continuous gelatin films	1099:1122	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	4	15	theme	composite	704:712	arg1	films					714:718	resultant composite films	694:718	resultant composite films	694:718	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	2	16	theme	CMC	510:512	arg1	MRs					514:516	different gelatin: CMC MRs	491:516	different gelatin: CMC MRs	491:516	The interaction between biopolymers was studied using turbidometric analysis at different gelatin: CMC MRs and pH values.
29555510	4	17	theme	control	761:767	arg1	films					777:781	control gelatin films	761:781	control gelatin films	761:781	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	1	18	theme	electrostatic	167:179	arg1	interactions					181:192	the electrostatic interactions	163:192	the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR)	163:284	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	7	19	dep	significantly	1039:1051	arg1	P≤0.05					1054:1059	P≤0.05	1054:1059	P≤0.05	1054:1059	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	2	20	theme	turbidometric	465:477	arg1	analysis					479:486	turbidometric analysis	465:486	turbidometric analysis	465:486	The interaction between biopolymers was studied using turbidometric analysis at different gelatin: CMC MRs and pH values.
29555510	8	21	theme	strength	1336:1343	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	21	theme	strength	1336:1343	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	21	theme	strength	1336:1343	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	21	theme	strength	1336:1343	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	21	theme	strength	1336:1343	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	21	theme	strength	1336:1343	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	22	theme	interaction	1213:1223	arg1	interaction					1213:1223	interaction	1213:1223	interaction	1213:1223	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	22	theme	interaction	1213:1223	arg1	amount					1203:1208	the maximum amount	1191:1208	the maximum amount of interaction	1191:1223	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	23	theme	pHopt	1167:1171	arg1	MR					1153:1154	MR	1153:1154	MR of 9:1 and pHopt (corresponding to the maximum amount of interaction)	1153:1224	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	1	24	theme	gelatin-CMC	347:357	arg1	films					369:373	gelatin-CMC composite films	347:373	gelatin-CMC composite films	347:373	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	6	25	theme	solubility	971:980	arg1	networks					954:961	resistant film networks	939:961	resistant film networks of less solubility and swellability	939:997	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	8	26	theme	different	1235:1243	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	characteristics					1245:1259	different characteristics	1235:1259	different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility	1235:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	26	theme	different	1235:1243	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	0	27	theme	Physical	0:7	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of gelatin-CMC composite films	0:64	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	1	28	theme	mixing	268:273	arg1	MR					282:283	MR	282:283	MR	282:283	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	1	28	theme	mixing	268:273	arg1	ratio					275:279	mixing ratio	268:279	mixing ratio (MR)	268:284	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	8	29	theme	solubility	1349:1358	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	29	theme	solubility	1349:1358	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	29	theme	solubility	1349:1358	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	29	theme	solubility	1349:1358	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	29	theme	solubility	1349:1358	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	29	theme	solubility	1349:1358	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	1	30	theme	ratio	275:279	arg1	function					249:256	a function	247:256	a function of pH and mixing ratio (MR)	247:284	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	8	31	theme	maximum	1195:1201	arg1	interaction					1213:1223	interaction	1213:1223	interaction	1213:1223	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	31	theme	maximum	1195:1201	arg1	amount					1203:1208	the maximum amount	1191:1208	the maximum amount of interaction	1191:1223	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	5	32	theme	interactions	812:823	arg1	intensity					799:807	the intensity	795:807	the intensity of interactions between the two biopolymers	795:851	Changes in the intensity of interactions between the two biopolymers resulted in films with different properties.
29555510	0	33	theme	mechanical	13:22	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of gelatin-CMC composite films	0:64	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	1	34	theme	composite	359:367	arg1	films					369:373	gelatin-CMC composite films	347:373	gelatin-CMC composite films	347:373	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	8	35	theme	minimum	1309:1315	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	35	theme	minimum	1309:1315	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	35	theme	minimum	1309:1315	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	35	theme	minimum	1309:1315	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	35	theme	minimum	1309:1315	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	1	36	theme	carboxymethyl	214:226	arg1	CMC					239:241	CMC	239:241	CMC	239:241	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	1	36	theme	carboxymethyl	214:226	arg1	cellulose					228:236	carboxymethyl cellulose	214:236	carboxymethyl cellulose (CMC)	214:242	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	0	37	theme	gelatin-CMC	38:48	arg1	films					60:64	gelatin-CMC composite films	38:64	gelatin-CMC composite films	38:64	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	6	38	theme	networks	954:961	arg1	formation					926:934	formation	926:934	formation of resistant film networks of less solubility and swellability	926:997	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	1	39	theme	films	369:373	arg1	properties					333:342	the physical and mechanical properties	305:342	the physical and mechanical properties of gelatin-CMC composite films	305:373	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	8	40	theme	maximum	1269:1275	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	40	theme	maximum	1269:1275	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	40	theme	maximum	1269:1275	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	40	theme	maximum	1269:1275	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	40	theme	maximum	1269:1275	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	6	41	theme	film	949:952	arg1	networks					954:961	resistant film networks	939:961	resistant film networks of less solubility and swellability	939:997	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	2	42	theme	pH	522:523	arg1	values					525:530	pH values	522:530	pH values	522:530	The interaction between biopolymers was studied using turbidometric analysis at different gelatin: CMC MRs and pH values.
29555510	3	43	theme	electrostatic	571:583	arg1	interactions					585:596	the electrostatic interactions	567:596	the electrostatic interactions	567:596	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	8	44	theme	WVP	1288:1290	arg1	solubility					1349:1358	solubility	1349:1358	solubility	1349:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	44	theme	WVP	1288:1290	arg1	amounts					1317:1323	minimum amounts	1309:1323	minimum amounts of tensile strength and solubility	1309:1358	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	44	theme	WVP	1288:1290	arg1	amounts					1277:1283	maximum amounts	1269:1283	maximum amounts of WVP and swelling	1269:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	44	theme	WVP	1288:1290	arg1	WVP					1288:1290	WVP	1288:1290	WVP	1288:1290	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	44	theme	WVP	1288:1290	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	44	theme	WVP	1288:1290	arg1	swelling					1296:1303	swelling	1296:1303	swelling	1296:1303	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	0	45	theme	films	60:64	arg1	properties					24:33	Physical and mechanical properties	0:33	Physical and mechanical properties of gelatin-CMC composite films	0:64	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	6	46	theme	resistant	939:947	arg1	networks					954:961	resistant film networks	939:961	resistant film networks of less solubility and swellability	939:997	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	8	47	theme	tensile	1328:1334	arg1	strength					1336:1343	tensile strength	1328:1343	tensile strength	1328:1343	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	8	48	theme	Composite	1125:1133	arg1	films					1135:1139	Composite films	1125:1139	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction)	1125:1224	Composite films prepared at MR of 9:1 and pHopt (corresponding to the maximum amount of interaction) revealed different characteristics such as maximum amounts of WVP and swelling and minimum amounts of tensile strength and solubility.
29555510	4	49	theme	resultant	694:702	arg1	films					714:718	resultant composite films	694:718	resultant composite films	694:718	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	0	50	theme	composite	50:58	arg1	films					60:64	gelatin-CMC composite films	38:64	gelatin-CMC composite films	38:64	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	9	51	theme	FTIR	1361:1364	arg1	spectra					1366:1372	FTIR spectra	1361:1372	FTIR spectra of composite films	1361:1391	FTIR spectra of composite films confirmed that gelatin and CMC were not covalently bonded.
29555510	5	52	with	films	865:869	arg1	properties					886:895	different properties	876:895	different properties	876:895	Changes in the intensity of interactions between the two biopolymers resulted in films with different properties.
29555510	3	53	theme	system	648:653	arg1	viscosity					629:637	the relative viscosity	616:637	the relative viscosity of mixed system	616:653	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	7	54	theme	vapor	1006:1010	arg1	permeability					1012:1023	Water vapor permeability	1000:1023	Water vapor permeability (WVP)	1000:1029	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	7	54	theme	vapor	1006:1010	arg1	WVP					1026:1028	WVP	1026:1028	WVP	1026:1028	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	3	55	from	reduction	535:543	arg1	MR					555:556	MR	555:556	MR	555:556	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	3	55	from	reduction	535:543	arg1	pH					548:549	pH	548:549	pH	548:549	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	0	56	dep	properties	24:33	arg1	influence					76:84	the influence	72:84	the influence of electrostatic interactions	72:114	Physical and mechanical properties of gelatin-CMC composite films under the influence of electrostatic interactions.
29555510	1	57	theme	current	134:140	arg1	study					142:146	current study	134:146	current study	134:146	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	6	58	theme	swellability	986:997	arg1	networks					954:961	resistant film networks	939:961	resistant film networks of less solubility and swellability	939:997	Polymer complexation led to formation of resistant film networks of less solubility and swellability.
29555510	4	59	theme	gelatin	769:775	arg1	films					777:781	control gelatin films	761:781	control gelatin films	761:781	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	1	60	theme	study	142:146	arg1	objective					121:129	The objective	117:129	The objective of current study	117:146	The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions.
29555510	4	61	theme	films	714:718	arg1	properties					680:689	Physical and mechanical properties	656:689	Physical and mechanical properties of resultant composite films	656:718	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
29555510	2	62	theme	gelatin	501:507	arg1	MRs					514:516	different gelatin: CMC MRs	491:516	different gelatin: CMC MRs	491:516	The interaction between biopolymers was studied using turbidometric analysis at different gelatin: CMC MRs and pH values.
29555510	3	63	theme	mixed	642:646	arg1	system					648:653	mixed system	642:653	mixed system	642:653	A reduction in pH and MR enhanced the electrostatic interactions; while, decreased the relative viscosity of mixed system.
29555510	7	64	theme	Water	1000:1004	arg1	permeability					1012:1023	Water vapor permeability	1000:1023	Water vapor permeability (WVP)	1000:1029	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	7	64	theme	Water	1000:1004	arg1	WVP					1026:1028	WVP	1026:1028	WVP	1026:1028	Water vapor permeability (WVP) was not significantly (P≤0.05) influenced by incorporating CMC into continuous gelatin films.
29555510	9	65	theme	films	1387:1391	arg1	spectra					1366:1372	FTIR spectra	1361:1372	FTIR spectra of composite films	1361:1391	FTIR spectra of composite films confirmed that gelatin and CMC were not covalently bonded.
29555510	2	66	theme	different	491:499	arg1	MRs					514:516	different gelatin: CMC MRs	491:516	different gelatin: CMC MRs	491:516	The interaction between biopolymers was studied using turbidometric analysis at different gelatin: CMC MRs and pH values.
29555510	4	67	theme	Physical	656:663	arg1	properties					680:689	Physical and mechanical properties	656:689	Physical and mechanical properties of resultant composite films	656:718	Physical and mechanical properties of resultant composite films were examined and compared with those of control gelatin films.
30789029	0	0	theme	quick	80:84	arg1	insight					97:103	quick and direct insight	80:103	quick and direct insight of almonds	80:114	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	6	1	from	evaluation	992:1001	arg1	almond					1034:1039	the almond	1030:1039	the almond	1030:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	4	2	theme	chemical	695:702	arg1	profiles					704:711	chemical profiles	695:711	chemical profiles featuring almond samples	695:736	This very rough approach is effectively providing chemical profiles featuring almond samples.
30789029	2	3	theme	samples	465:471	arg1	composition					436:446	the chemical composition	423:446	the chemical composition of powdered seed samples dispersed in D2O	423:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	6	4	theme	first	894:898	arg1	investigation					900:912	a first investigation	892:912	a first investigation based on the main compounds	892:940	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	6	4	theme	first	894:898	arg1	This					879:882	This	879:882	This	879:882	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	3	5	theme	1H	491:492	arg1	spectra					494:500	1H spectra	491:500	1H spectra	491:500	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	0	6	theme	direct	90:95	arg1	insight					97:103	quick and direct insight	80:103	quick and direct insight of almonds	80:114	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	1	7	theme	confectionery	322:334	arg1	products					336:343	the confectionery products	318:343	the confectionery products	318:343	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	6	8	from	almond	1034:1039	arg1	evaluation					992:1001	the quantitative evaluation	975:1001	the quantitative evaluation of many other compounds in the almond	975:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	1	9	theme	products	336:343	arg1	ingredient					304:313	basic ingredient	298:313	basic ingredient of the confectionery products	298:343	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	1	9	theme	products	336:343	arg1	it					264:265	it	264:265	it	264:265	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	4	10	theme	almond	723:728	arg1	samples					730:736	almond samples	723:736	almond samples	723:736	This very rough approach is effectively providing chemical profiles featuring almond samples.
30789029	5	11	theme	Avola	803:807	arg1	almonds					810:816	the "Avola" almonds	798:816	the "Avola" almonds from other marketed products submitted to the same analysis	798:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	5	12	theme	same	864:867	arg1	analysis					869:876	the same analysis	860:876	the same analysis	860:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	1	13	theme	Prunus	148:153	arg1	plants					162:167	Prunus dulcis plants	148:167	Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value	148:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	2	14	theme	seed	460:463	arg1	samples					465:471	powdered seed samples	451:471	powdered seed samples dispersed in D2O	451:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	6	15	from	compounds	1017:1025	arg1	almond					1034:1039	the almond	1030:1039	the almond	1030:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	3	16	theme	remarkable	514:523	arg1	presence					525:532	the remarkable presence	510:532	the remarkable presence of triglyceride fatty esters together with sucrose	510:583	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	6	17	theme	HR-MAS-NMR	1068:1077	arg1	knowledge					1079:1087	the HR-MAS-NMR knowledge	1064:1087	the HR-MAS-NMR knowledge of these precious seeds	1064:1111	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	1	18	theme	dulcis	155:160	arg1	plants					162:167	Prunus dulcis plants	148:167	Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value	148:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	0	19	theme	magic	16:20	arg1	angle					22:26	High-resolution magic angle	0:26	High-resolution magic angle	0:26	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	3	20	theme	esters	556:561	arg1	presence					525:532	the remarkable presence	510:532	the remarkable presence of triglyceride fatty esters together with sucrose	510:583	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	1	21	theme	plants	162:167	arg1	Almonds					117:123	Almonds	117:123	Almonds	117:123	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	1	21	theme	plants	162:167	arg1	seeds					139:143	the tasty seeds	129:143	the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value	129:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	0	22	theme	High-resolution	0:14	arg1	angle					22:26	High-resolution magic angle	0:26	High-resolution magic angle	0:26	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	4	23	theme	rough	655:659	arg1	approach					661:668	This very rough approach	645:668	This very rough approach	645:668	This very rough approach is effectively providing chemical profiles featuring almond samples.
30789029	5	24	theme	"	808:808	arg1	almonds					810:816	the "Avola" almonds	798:816	the "Avola" almonds from other marketed products submitted to the same analysis	798:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	3	25	theme	triglyceride	537:548	arg1	esters					556:561	triglyceride fatty esters	537:561	triglyceride fatty esters together with sucrose	537:583	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	5	26	theme	other	823:827	arg1	products					838:845	other marketed products	823:845	other marketed products submitted to the same analysis	823:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	6	27	theme	precious	1098:1105	arg1	seeds					1107:1111	these precious seeds	1092:1111	these precious seeds	1092:1111	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	0	28	theme	almonds	108:114	arg1	insight					97:103	quick and direct insight	80:103	quick and direct insight of almonds	80:114	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	3	29	theme	fatty	550:554	arg1	esters					556:561	triglyceride fatty esters	537:561	triglyceride fatty esters together with sucrose	537:583	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	5	30	theme	marketed	829:836	arg1	products					838:845	other marketed products	823:845	other marketed products submitted to the same analysis	823:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	0	31	theme	magnetic	45:52	arg1	HR-MAS-NMR					65:74	HR-MAS-NMR	65:74	HR-MAS-NMR	65:74	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	0	31	theme	magnetic	45:52	arg1	resonance					54:62	nuclear magnetic resonance	37:62	nuclear magnetic resonance (HR-MAS-NMR)	37:75	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	5	32	from	analysis	747:754	arg1	able					764:767	able	764:767	able	764:767	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	6	33	theme	compounds	1017:1025	arg1	evaluation					992:1001	the quantitative evaluation	975:1001	the quantitative evaluation of many other compounds in the almond	975:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	0	34	theme	nuclear	37:43	arg1	HR-MAS-NMR					65:74	HR-MAS-NMR	65:74	HR-MAS-NMR	65:74	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	0	34	theme	nuclear	37:43	arg1	resonance					54:62	nuclear magnetic resonance	37:62	nuclear magnetic resonance (HR-MAS-NMR)	37:75	High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS-NMR) as quick and direct insight of almonds.
30789029	2	35	theme	powdered	451:458	arg1	samples					465:471	powdered seed samples	451:471	powdered seed samples dispersed in D2O	451:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	3	36	theme	other	586:590	arg1	metabolites					612:622	other minor water soluble metabolites	586:622	other minor water soluble metabolites	586:622	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	5	37	from	products	838:845	arg1	almonds					810:816	the "Avola" almonds	798:816	the "Avola" almonds from other marketed products submitted to the same analysis	798:876	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	1	38	theme	pleasant	198:205	arg1	palatability					207:218	the pleasant palatability	194:218	the pleasant palatability	194:218	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	6	39	theme	main	927:930	arg1	compounds					932:940	the main compounds	923:940	the main compounds	923:940	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	6	40	theme	other	1011:1015	arg1	compounds					1017:1025	many other compounds	1006:1025	many other compounds in the almond	1006:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	5	41	from	able	764:767	arg1	analysis					747:754	this analysis	742:754	this analysis	742:754	In this analysis we were able to statistically distinguish the "Avola" almonds from other marketed products submitted to the same analysis.
30789029	1	42	theme	basic	298:302	arg1	ingredient					304:313	basic ingredient	298:313	basic ingredient of the confectionery products	298:343	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	1	42	theme	basic	298:302	arg1	it					264:265	it	264:265	it	264:265	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	3	43	theme	soluble	604:610	arg1	metabolites					612:622	other minor water soluble metabolites	586:622	other minor water soluble metabolites	586:622	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	6	44	theme	seeds	1107:1111	arg1	knowledge					1079:1087	the HR-MAS-NMR knowledge	1064:1087	the HR-MAS-NMR knowledge of these precious seeds	1064:1111	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	6	45	theme	many	1006:1009	arg1	compounds					1017:1025	many other compounds	1006:1025	many other compounds in the almond	1006:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	1	46	theme	tasty	133:137	arg1	Almonds					117:123	Almonds	117:123	Almonds	117:123	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	1	46	theme	tasty	133:137	arg1	seeds					139:143	the tasty seeds	129:143	the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value	129:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	2	47	theme	able	386:389	arg1	spectroscopy					373:384	a simple spectroscopy	364:384	a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O	364:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	2	47	theme	able	386:389	arg1	HR-MAS-NMR					350:359	The HR-MAS-NMR	346:359	The HR-MAS-NMR	346:359	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	3	48	theme	minor	592:596	arg1	metabolites					612:622	other minor water soluble metabolites	586:622	other minor water soluble metabolites	586:622	1H spectra witness the remarkable presence of triglyceride fatty esters together with sucrose; other minor water soluble metabolites are also detectable.
30789029	2	49	theme	simple	366:371	arg1	spectroscopy					373:384	a simple spectroscopy	364:384	a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O	364:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	2	49	theme	simple	366:371	arg1	HR-MAS-NMR					350:359	The HR-MAS-NMR	346:359	The HR-MAS-NMR	346:359	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	1	50	theme	remarkable	224:233	arg1	value					247:251	remarkable nutritional value	224:251	remarkable nutritional value	224:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30789029	6	51	theme	quantitative	979:990	arg1	evaluation					992:1001	the quantitative evaluation	975:1001	the quantitative evaluation of many other compounds in the almond	975:1039	This is just a first investigation based on the main compounds but it might pave the way toward the quantitative evaluation of many other compounds in the almond therefore implementing the HR-MAS-NMR knowledge of these precious seeds.
30789029	2	52	theme	chemical	427:434	arg1	composition					436:446	the chemical composition	423:446	the chemical composition of powdered seed samples dispersed in D2O	423:488	The HR-MAS-NMR is a simple spectroscopy able to directly and quickly explore the chemical composition of powdered seed samples dispersed in D2O.
30789029	1	53	theme	nutritional	235:245	arg1	value					247:251	remarkable nutritional value	224:251	remarkable nutritional value	224:251	Almonds are the tasty seeds of Prunus dulcis plants globally appreciated for the pleasant palatability and remarkable nutritional value, therefore it is very spread as snack and as basic ingredient of the confectionery products.
30241841	10	0	theme	hydrogel	1432:1439	arg1	compositions					1441:1452	the hydrogel compositions	1428:1452	the hydrogel compositions	1428:1452	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	12	1	theme	bio-compatible	1653:1666	arg1	hydrogels					1686:1694	more bio-compatible thermo-responsive hydrogels	1648:1694	more bio-compatible thermo-responsive hydrogels	1648:1694	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	11	2	theme	in-vivo	1461:1467	arg1	biocompatibility					1469:1484	Their in-vivo biocompatibility	1455:1484	Their in-vivo biocompatibility	1455:1484	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	8	3	theme	phase	1081:1085	arg1	VPTT					1111:1114	VPTT	1111:1114	VPTT	1111:1114	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	3	theme	phase	1081:1085	arg1	temperature					1098:1108	Volume phase transition temperature	1074:1108	Volume phase transition temperature (VPTT) of the hydrogels	1074:1132	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	11	4	theme	buffer	1545:1550	arg1	saline					1552:1557	phosphate buffer saline	1535:1557	phosphate buffer saline (PBS, pH 7.4)	1535:1571	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	7	5	theme	thermogravimetric	881:897	arg1	TGA					909:911	TGA	909:911	TGA	909:911	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	7	5	theme	thermogravimetric	881:897	arg1	analysis					899:906	thermogravimetric analysis	881:906	thermogravimetric analysis (TGA)	881:912	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	4	6	theme	different	554:562	arg1	temperatures					564:575	different temperatures	554:575	different temperatures	554:575	Swelling behavior of the hydrogels at different temperatures was investigated.
30241841	8	7	theme	differential	1173:1184	arg1	DDSC					1208:1211	DDSC	1208:1211	DDSC	1208:1211	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	7	theme	differential	1173:1184	arg1	calorimeter					1195:1205	derivative differential scanning calorimeter	1162:1205	derivative differential scanning calorimeter (DDSC)	1162:1212	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	7	8	theme	interaction	953:963	arg1	extent					928:933	the extent	924:933	the extent of intermolecular interaction between PNIPAM and β-glucan	924:991	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	7	8	theme	interaction	953:963	arg1	proportional					996:1007	proportional	996:1007	proportional	996:1007	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	5	9	theme	UV-VIS	652:657	arg1	spectrophotometer					659:675	UV-VIS spectrophotometer	652:675	UV-VIS spectrophotometer	652:675	The 5-ASA release from the hydrogels was monitored using UV-VIS spectrophotometer at 37 °C.
30241841	5	10	theme	5-ASA	599:603	arg1	release					605:611	The 5-ASA release	595:611	The 5-ASA release from the hydrogels	595:630	The 5-ASA release from the hydrogels was monitored using UV-VIS spectrophotometer at 37 °C.
30241841	11	11	theme	human	1509:1513	arg1	cells					1526:1530	WS1 human fibroblast cells	1505:1530	WS1 human fibroblast cells	1505:1530	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	9	12	contain	possessed	1220:1228	arg2	VPTT					1239:1242	variable VPTT	1230:1242	variable VPTT	1230:1242	They possessed variable VPTT with the compositions.
30241841	9	12	contain	possessed	1220:1228	arg1	They					1215:1218	They	1215:1218	They	1215:1218	They possessed variable VPTT with the compositions.
30241841	8	13	theme	derivative	1162:1171	arg1	DDSC					1208:1211	DDSC	1208:1211	DDSC	1208:1211	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	13	theme	derivative	1162:1171	arg1	calorimeter					1195:1205	derivative differential scanning calorimeter	1162:1205	derivative differential scanning calorimeter (DDSC)	1162:1212	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	2	14	used	used	326:329	arg2	KPS					316:318	KPS	316:318	KPS	316:318	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	14	used	used	326:329	arg2	TEMED					283:287	TEMED	283:287	TEMED	283:287	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	14	used	used	326:329	arg2	Tetramethylethylenediamine					255:280	Tetramethylethylenediamine	255:280	Tetramethylethylenediamine (TEMED)	255:288	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	14	used	used	326:329	arg2	persulfate					304:313	potassium persulfate	294:313	potassium persulfate (KPS)	294:319	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	14	used	used	326:329	arg2	pair					342:345	a redox pair	334:345	a redox pair	334:345	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	4	15	from	temperatures	564:575	arg1	behavior					525:532	Swelling behavior	516:532	Swelling behavior of the hydrogels at different temperatures	516:575	Swelling behavior of the hydrogels at different temperatures was investigated.
30241841	11	16	theme	phosphate	1535:1543	arg1	saline					1552:1557	phosphate buffer saline	1535:1557	phosphate buffer saline (PBS, pH 7.4)	1535:1571	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	3	17	used	used	393:396	arg2	β-glucan					348:355	β-glucan	348:355	β-glucan	348:355	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	3	17	used	used	393:396	arg2	cross-linker					436:447	a biodegradable and bio-compatible cross-linker	401:447	a biodegradable and bio-compatible cross-linker	401:447	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	10	18	theme	PNIPAM	1299:1304	arg1	network					1306:1312	the PNIPAM network	1295:1312	the PNIPAM network	1295:1312	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	8	19	theme	scanning	1186:1193	arg1	DDSC					1208:1211	DDSC	1208:1211	DDSC	1208:1211	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	19	theme	scanning	1186:1193	arg1	calorimeter					1195:1205	derivative differential scanning calorimeter	1162:1205	derivative differential scanning calorimeter (DDSC)	1162:1212	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	11	20	theme	fibroblast	1515:1524	arg1	cells					1526:1530	WS1 human fibroblast cells	1505:1530	WS1 human fibroblast cells	1505:1530	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	7	21	theme	intermolecular	938:951	arg1	interaction					953:963	intermolecular interaction	938:963	intermolecular interaction between PNIPAM and β-glucan	938:991	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	10	22	dep	35.5 °C	1375:1381	arg1	to					1372:1373	to	1372:1373	to	1372:1373	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	3	23	dep	methacrylated	361:373	arg1	MA-β-Glucan					376:386	MA-β-Glucan	376:386	MA-β-Glucan	376:386	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	5	24	from	hydrogels	622:630	arg1	release					605:611	The 5-ASA release	595:611	The 5-ASA release from the hydrogels	595:630	The 5-ASA release from the hydrogels was monitored using UV-VIS spectrophotometer at 37 °C.
30241841	10	25	from	presence	1271:1278	arg1	network					1306:1312	the PNIPAM network	1295:1312	the PNIPAM network	1295:1312	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	2	26	theme	redox	336:340	arg1	pair					342:345	a redox pair	334:345	a redox pair	334:345	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	26	theme	redox	336:340	arg1	Tetramethylethylenediamine					255:280	Tetramethylethylenediamine	255:280	Tetramethylethylenediamine (TEMED)	255:288	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	26	theme	redox	336:340	arg1	persulfate					304:313	potassium persulfate	294:313	potassium persulfate (KPS)	294:319	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	1	27	theme	room	237:240	arg1	temperature					242:252	room temperature	237:252	room temperature	237:252	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	8	28	theme	Volume	1074:1079	arg1	VPTT					1111:1114	VPTT	1111:1114	VPTT	1111:1114	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	28	theme	Volume	1074:1079	arg1	temperature					1098:1108	Volume phase transition temperature	1074:1108	Volume phase transition temperature (VPTT) of the hydrogels	1074:1132	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	9	29	theme	variable	1230:1237	arg1	VPTT					1239:1242	variable VPTT	1230:1242	variable VPTT	1230:1242	They possessed variable VPTT with the compositions.
30241841	1	30	theme	responsive	95:104	arg1	hydrogels					106:114	temperature responsive hydrogels	83:114	temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM)	83:192	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	0	31	theme	responsive	30:39	arg1	hydrogels					41:49	temperature responsive hydrogels	18:49	temperature responsive hydrogels	18:49	Beta-Glucan based temperature responsive hydrogels for 5-ASA delivery.
30241841	12	32	theme	swollen	1754:1760	arg1	surface					1771:1777	the swollen hydrogel surface	1750:1777	the swollen hydrogel surface	1750:1777	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	1	33	theme	hydrogels	106:114	arg1	series					73:78	A series	71:78	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM)	71:192	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	7	34	theme	thermal	842:848	arg1	stability					850:858	Their thermal stability	836:858	Their thermal stability	836:858	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	1	35	theme	N-isopropyl	161:171	arg1	poly					155:158	poly	155:158	poly (N-isopropyl acrylamide) (PNIPAM)	155:192	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	1	35	theme	N-isopropyl	161:171	arg1	acrylamide					173:182	N-isopropyl acrylamide	161:182	N-isopropyl acrylamide	161:182	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	7	36	theme	thermal	1012:1018	arg1	stability					1020:1028	thermal stability	1012:1028	thermal stability	1012:1028	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	8	37	theme	transition	1087:1096	arg1	VPTT					1111:1114	VPTT	1111:1114	VPTT	1111:1114	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	37	theme	transition	1087:1096	arg1	temperature					1098:1108	Volume phase transition temperature	1074:1108	Volume phase transition temperature (VPTT) of the hydrogels	1074:1132	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	4	38	theme	hydrogels	541:549	arg1	behavior					525:532	Swelling behavior	516:532	Swelling behavior of the hydrogels at different temperatures	516:575	Swelling behavior of the hydrogels at different temperatures was investigated.
30241841	7	39	theme	PNIPAM	1066:1071	arg1	amount					1056:1061	the amount	1052:1061	the amount of PNIPAM	1052:1071	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	7	39	theme	PNIPAM	1066:1071	arg1	PNIPAM					1066:1071	PNIPAM	1066:1071	PNIPAM	1066:1071	Their thermal stability was determined using thermogravimetric analysis (TGA), assuming the extent of intermolecular interaction between PNIPAM and β-glucan is proportional to thermal stability, which increased with the amount of PNIPAM.
30241841	11	40	theme	WS1	1505:1507	arg1	cells					1526:1530	WS1 human fibroblast cells	1505:1530	WS1 human fibroblast cells	1505:1530	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	10	41	from	35.5 °C	1375:1381	arg1	temperature					1346:1356	the body temperature	1337:1356	the body temperature (from 32.8 °C to 35.5 °C)	1337:1382	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	12	42	theme	thermo-responsive	1668:1684	arg1	hydrogels					1686:1694	more bio-compatible thermo-responsive hydrogels	1648:1694	more bio-compatible thermo-responsive hydrogels	1648:1694	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	1	43	theme	redox	213:217	arg1	polymerization					219:232	redox polymerization	213:232	redox polymerization	213:232	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	6	44	theme	hydrogels	749:757	arg1	behaviors					732:740	the swelling and release behaviors	707:740	the swelling and release behaviors of the hydrogels	707:757	It is notable that, the swelling and release behaviors of the hydrogels significantly change depending on the hydrogel compositions and temperature.
30241841	6	45	theme	hydrogel	797:804	arg1	compositions					806:817	the hydrogel compositions	793:817	the hydrogel compositions	793:817	It is notable that, the swelling and release behaviors of the hydrogels significantly change depending on the hydrogel compositions and temperature.
30241841	10	46	attach	presence	1271:1278	arg2	β-glucan					1283:1290	β-glucan	1283:1290	β-glucan	1283:1290	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	10	46	attach	presence	1271:1278	arg1	network					1306:1312	the PNIPAM network	1295:1312	the PNIPAM network	1295:1312	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	12	47	from	enhancement	1711:1721	arg1	surface					1771:1777	the swollen hydrogel surface	1750:1777	the swollen hydrogel surface	1750:1777	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	0	48	theme	5-ASA	55:59	arg1	delivery					61:68	5-ASA delivery	55:68	5-ASA delivery	55:68	Beta-Glucan based temperature responsive hydrogels for 5-ASA delivery.
30241841	12	49	theme	β-Glucan	1738:1745	arg1	enhancement					1711:1721	the enhancement	1707:1721	the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface	1707:1777	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	10	50	theme	body	1341:1344	arg1	temperature					1346:1356	the body temperature	1337:1356	the body temperature (from 32.8 °C to 35.5 °C)	1337:1382	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30241841	4	51	theme	Swelling	516:523	arg1	behavior					525:532	Swelling behavior	516:532	Swelling behavior of the hydrogels at different temperatures	516:575	Swelling behavior of the hydrogels at different temperatures was investigated.
30241841	8	52	theme	hydrogels	1124:1132	arg1	VPTT					1111:1114	VPTT	1111:1114	VPTT	1111:1114	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	8	52	theme	hydrogels	1124:1132	arg1	temperature					1098:1108	Volume phase transition temperature	1074:1108	Volume phase transition temperature (VPTT) of the hydrogels	1074:1132	Volume phase transition temperature (VPTT) of the hydrogels was precisely determined by derivative differential scanning calorimeter (DDSC).
30241841	12	53	theme	hydrogel	1762:1769	arg1	surface					1771:1777	the swollen hydrogel surface	1750:1777	the swollen hydrogel surface	1750:1777	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	1	54	theme	1,3	131:133	arg1	β-Glucan					142:149	(1,3)-(1,6) β-Glucan	130:149	(1,3)-(1,6) β-Glucan	130:149	A series of temperature responsive hydrogels consisting of (1,3)-(1,6) β-Glucan and poly (N-isopropyl acrylamide) (PNIPAM) was synthesized by redox polymerization at room temperature.
30241841	6	55	theme	release	724:730	arg1	behaviors					732:740	the swelling and release behaviors	707:740	the swelling and release behaviors of the hydrogels	707:757	It is notable that, the swelling and release behaviors of the hydrogels significantly change depending on the hydrogel compositions and temperature.
30241841	2	56	theme	potassium	294:302	arg1	pair					342:345	a redox pair	334:345	a redox pair	334:345	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	56	theme	potassium	294:302	arg1	Tetramethylethylenediamine					255:280	Tetramethylethylenediamine	255:280	Tetramethylethylenediamine (TEMED)	255:288	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	56	theme	potassium	294:302	arg1	KPS					316:318	KPS	316:318	KPS	316:318	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	2	56	theme	potassium	294:302	arg1	persulfate					304:313	potassium persulfate	294:313	potassium persulfate (KPS)	294:319	Tetramethylethylenediamine (TEMED) and potassium persulfate (KPS) were used as a redox pair.
30241841	12	57	theme	hydrophilic	1726:1736	arg1	β-Glucan					1738:1745	hydrophilic β-Glucan	1726:1745	hydrophilic β-Glucan	1726:1745	It was demonstrated that, using MA-β-glucan as a cross-linker resulted in more bio-compatible thermo-responsive hydrogels indicating the enhancement of hydrophilic β-Glucan on the swollen hydrogel surface.
30241841	3	58	theme	bio-compatible	421:434	arg1	cross-linker					436:447	a biodegradable and bio-compatible cross-linker	401:447	a biodegradable and bio-compatible cross-linker	401:447	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	3	58	theme	bio-compatible	421:434	arg1	β-glucan					348:355	β-glucan	348:355	β-glucan	348:355	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	3	59	theme	biodegradable	403:415	arg1	cross-linker					436:447	a biodegradable and bio-compatible cross-linker	401:447	a biodegradable and bio-compatible cross-linker	401:447	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	3	59	theme	biodegradable	403:415	arg1	β-glucan					348:355	β-glucan	348:355	β-glucan	348:355	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	11	60	dep	saline	1552:1557	arg1	PBS					1560:1562	PBS	1560:1562	PBS	1560:1562	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	11	60	dep	saline	1552:1557	arg1	pH					1565:1566	pH 7.4	1565:1570	pH 7.4	1565:1570	Their in-vivo biocompatibility was tested against WS1 human fibroblast cells in phosphate buffer saline (PBS, pH 7.4).
30241841	6	61	theme	swelling	711:718	arg1	behaviors					732:740	the swelling and release behaviors	707:740	the swelling and release behaviors of the hydrogels	707:757	It is notable that, the swelling and release behaviors of the hydrogels significantly change depending on the hydrogel compositions and temperature.
30241841	3	62	theme	responsive	494:503	arg1	hydrogels					505:513	β-glucan-PNIPAM based temperature responsive hydrogels	460:513	β-glucan-PNIPAM based temperature responsive hydrogels	460:513	β-glucan was methacrylated (MA-β-Glucan) and used as a biodegradable and bio-compatible cross-linker to prepare β-glucan-PNIPAM based temperature responsive hydrogels.
30241841	10	63	theme	β-glucan	1283:1290	arg1	presence					1271:1278	The presence	1267:1278	The presence of β-glucan in the PNIPAM network	1267:1312	The presence of β-glucan in the PNIPAM network brought VPTT closer to the body temperature (from 32.8 °C to 35.5 °C), indicating that the VPTT could be tuned by the hydrogel compositions.
30525515	10	0	theme	facile	1538:1543	arg1	method					1545:1550	A greener yet facile method	1524:1550	A greener yet facile method for producing CNF from vegetable waste	1524:1589	A greener yet facile method for producing CNF from vegetable waste is presented here.
30525515	8	1	used	used	1298:1301	arg2	rheology					1285:1292	Dynamic rheology	1277:1292	Dynamic rheology	1277:1292	Dynamic rheology was used to estimate the proportion of CNF in resultant suspensions.
30525515	7	2	theme	enzyme-pretreatment	1193:1211	arg1	effect					1179:1184	the effect	1175:1184	the effect of the enzyme-pretreatment	1175:1211	While effective alone, the effect of the enzyme-pretreatment was bolstered via combination with a mild high-pH pretreatment.
30525515	3	3	theme	large-scale	517:527	arg1	processing					529:538	large-scale processing	517:538	large-scale processing	517:538	However, this is expensive for large-scale processing and environmentally damaging.
30525515	10	4	theme	vegetable	1575:1583	arg1	waste					1585:1589	vegetable waste	1575:1589	vegetable waste	1575:1589	A greener yet facile method for producing CNF from vegetable waste is presented here.
30525515	9	5	theme	untreated	1505:1513	arg1	samples					1515:1521	untreated samples	1505:1521	untreated samples	1505:1521	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	4	6	from	byproducts	633:642	arg1	CNF					608:610	CNF	608:610	CNF from agro-industrial byproducts (potato and sugar beet)	608:666	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	6	7	theme	Polymer	938:944	arg1	Profiling					946:954	Comprehensive Microarray Polymer Profiling	913:954	Comprehensive Microarray Polymer Profiling (CoMPP)	913:962	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	6	7	theme	Polymer	938:944	arg1	CoMPP					957:961	CoMPP	957:961	CoMPP	957:961	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	10	8	theme	greener	1526:1532	arg1	method					1545:1550	A greener yet facile method	1524:1550	A greener yet facile method for producing CNF from vegetable waste	1524:1589	A greener yet facile method for producing CNF from vegetable waste is presented here.
30525515	9	9	theme	4-fold	1400:1405	arg1	increases					1419:1427	4-fold and 10-fold increases	1400:1427	4-fold and 10-fold increases in the storage modulus for potato and sugar beet	1400:1476	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	6	10	theme	Microarray	927:936	arg1	Profiling					946:954	Comprehensive Microarray Polymer Profiling	913:954	Comprehensive Microarray Polymer Profiling (CoMPP)	913:962	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	6	10	theme	Microarray	927:936	arg1	CoMPP					957:961	CoMPP	957:961	CoMPP	957:961	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	2	11	from	byproducts	342:351	arg1	production					310:319	Nanofiber production	300:319	Nanofiber production from agro-industrial byproducts	300:351	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	4	12	theme	agro-industrial	617:631	arg1	potato					645:650	potato	645:650	potato	645:650	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	12	theme	agro-industrial	617:631	arg1	beet					662:665	sugar beet	656:665	sugar beet	656:665	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	12	theme	agro-industrial	617:631	arg1	byproducts					633:642	agro-industrial byproducts	617:642	agro-industrial byproducts (potato and sugar beet)	617:666	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	6	13	theme	Comprehensive	913:925	arg1	Profiling					946:954	Comprehensive Microarray Polymer Profiling	913:954	Comprehensive Microarray Polymer Profiling (CoMPP)	913:962	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	6	13	theme	Comprehensive	913:925	arg1	CoMPP					957:961	CoMPP	957:961	CoMPP	957:961	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	1	14	from	phase	270:274	arg1	materials					289:297	composite materials	279:297	composite materials	279:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	3	15	theme	environmentally	544:558	arg1	damaging					560:567	environmentally damaging	544:567	environmentally damaging	544:567	However, this is expensive for large-scale processing and environmentally damaging.
30525515	1	16	theme	cellulose	193:201	arg1	phase					270:274	the reinforcing phase	254:274	the reinforcing phase in composite materials	254:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	1	16	theme	cellulose	193:201	arg1	CNF					215:217	CNF	215:217	CNF	215:217	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	1	16	theme	cellulose	193:201	arg1	nanofibers					203:212	cellulose nanofibers	193:212	cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials	193:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	4	17	theme	targeted	687:694	arg1	mixtures					714:721	targeted commercial enzyme mixtures	687:721	targeted commercial enzyme mixtures	687:721	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	1	18	theme	Cellulose	93:101	arg1	fibers					103:108	Cellulose fibers	93:108	Cellulose fibers	93:108	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	5	19	theme	CNF	878:880	arg1	liberation					864:873	greater liberation	856:873	greater liberation of CNF	856:880	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	2	20	theme	dry	473:475	arg1	weight					477:482	dry weight	473:482	dry weight	473:482	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	2	21	theme	Nanofiber	300:308	arg1	production					310:319	Nanofiber production	300:319	Nanofiber production from agro-industrial byproducts	300:351	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	7	22	theme	mild	1250:1253	arg1	pretreatment					1263:1274	a mild high-pH pretreatment	1248:1274	a mild high-pH pretreatment	1248:1274	While effective alone, the effect of the enzyme-pretreatment was bolstered via combination with a mild high-pH pretreatment.
30525515	2	23	theme	high	404:407	arg1	temperatures					409:420	high temperatures	404:420	high temperatures	404:420	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	5	24	theme	low	823:825	arg1	content					834:840	the low lignin content	819:840	the low lignin content	819:840	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	9	25	from	increases	1419:1427	arg1	modulus					1444:1450	the storage modulus	1432:1450	the storage modulus for potato and sugar beet	1432:1476	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	9	26	theme	storage	1436:1442	arg1	modulus					1444:1450	the storage modulus	1432:1450	the storage modulus for potato and sugar beet	1432:1476	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	4	27	theme	commercial	696:705	arg1	mixtures					714:721	targeted commercial enzyme mixtures	687:721	targeted commercial enzyme mixtures	687:721	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	0	28	theme	Agro-Industrial	25:39	arg1	Waste					47:51	Agro-Industrial Plant Waste	25:51	Agro-Industrial Plant Waste	25:51	Nanofibers Produced from Agro-Industrial Plant Waste Using Entirely Enzymatic Pretreatments.
30525515	1	29	theme	reinforcing	258:268	arg1	phase					270:274	the reinforcing phase	254:274	the reinforcing phase in composite materials	254:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	1	29	theme	reinforcing	258:268	arg1	nanofibers					203:212	cellulose nanofibers	193:212	cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials	193:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	8	30	theme	resultant	1340:1348	arg1	suspensions					1350:1360	resultant suspensions	1340:1360	resultant suspensions	1340:1360	Dynamic rheology was used to estimate the proportion of CNF in resultant suspensions.
30525515	8	31	theme	Dynamic	1277:1283	arg1	rheology					1285:1292	Dynamic rheology	1277:1292	Dynamic rheology	1277:1292	Dynamic rheology was used to estimate the proportion of CNF in resultant suspensions.
30525515	4	32	dep	byproducts	633:642	arg1	potato					645:650	potato	645:650	potato	645:650	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	32	dep	byproducts	633:642	arg1	beet					662:665	sugar beet	656:665	sugar beet	656:665	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	32	dep	byproducts	633:642	arg1	byproducts					633:642	agro-industrial byproducts	617:642	agro-industrial byproducts (potato and sugar beet)	617:666	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	33	theme	enzyme	707:712	arg1	mixtures					714:721	targeted commercial enzyme mixtures	687:721	targeted commercial enzyme mixtures	687:721	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	9	34	theme	10-fold	1411:1417	arg1	increases					1419:1427	4-fold and 10-fold increases	1400:1427	4-fold and 10-fold increases in the storage modulus for potato and sugar beet	1400:1476	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	5	35	theme	greater	856:862	arg1	liberation					864:873	greater liberation	856:873	greater liberation of CNF	856:880	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	5	36	theme	high-shear	886:895	arg1	homogenization					897:910	high-shear homogenization	886:910	high-shear homogenization	886:910	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	2	37	theme	noncellulosic	432:444	arg1	polysaccharides					446:460	noncellulosic polysaccharides	432:460	noncellulosic polysaccharides (20-70% of dry weight)	432:483	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	0	38	theme	Plant	41:45	arg1	Waste					47:51	Agro-Industrial Plant Waste	25:51	Agro-Industrial Plant Waste	25:51	Nanofibers Produced from Agro-Industrial Plant Waste Using Entirely Enzymatic Pretreatments.
30525515	1	39	theme	composite	279:287	arg1	materials					289:297	composite materials	279:297	composite materials	279:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	5	40	theme	lignin	827:832	arg1	content					834:840	the low lignin content	819:840	the low lignin content	819:840	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	5	41	theme	due	812:814	arg1	biomass					783:789	the biomass	779:789	the biomass	779:789	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	6	42	theme	remaining	973:981	arg1	polysaccharides					995:1009	remaining extractable polysaccharides	973:1009	remaining extractable polysaccharides	973:1009	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	4	43	theme	enzyme-only	573:583	arg1	pretreatment					585:596	An enzyme-only pretreatment	570:596	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet)	570:666	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	2	44	theme	weight	477:482	arg1	%					468:468	20-70%	463:468	20-70% of dry weight	463:482	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	2	44	theme	weight	477:482	arg1	weight					477:482	dry weight	473:482	dry weight	473:482	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	9	45	theme	sugar	1467:1471	arg1	beet					1473:1476	sugar beet	1467:1476	sugar beet	1467:1476	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	2	46	dep	polysaccharides	446:460	arg1	%					468:468	20-70%	463:468	20-70% of dry weight	463:482	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	2	46	dep	polysaccharides	446:460	arg1	weight					477:482	dry weight	473:482	dry weight	473:482	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	8	47	from	proportion	1319:1328	arg1	suspensions					1350:1360	resultant suspensions	1340:1360	resultant suspensions	1340:1360	Dynamic rheology was used to estimate the proportion of CNF in resultant suspensions.
30525515	2	48	theme	agro-industrial	326:340	arg1	byproducts					342:351	agro-industrial byproducts	326:351	agro-industrial byproducts	326:351	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	6	49	theme	extractable	983:993	arg1	polysaccharides					995:1009	remaining extractable polysaccharides	973:1009	remaining extractable polysaccharides	973:1009	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	1	50	used	used	232:235	arg2	CNF					215:217	CNF	215:217	CNF	215:217	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	1	50	used	used	232:235	arg2	nanofibers					203:212	cellulose nanofibers	193:212	cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials	193:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	1	50	used	used	232:235	arg2	phase					270:274	the reinforcing phase	254:274	the reinforcing phase in composite materials	254:297	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	8	51	theme	CNF	1333:1335	arg1	proportion					1319:1328	the proportion	1315:1328	the proportion of CNF in resultant suspensions	1315:1360	Dynamic rheology was used to estimate the proportion of CNF in resultant suspensions.
30525515	1	52	theme	cell-wall	132:140	arg1	skeleton					142:149	the cell-wall skeleton	128:149	the cell-wall skeleton	128:149	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	6	53	theme	noncellulosic	1081:1093	arg1	polysaccharides					1095:1109	noncellulosic polysaccharides	1081:1109	noncellulosic polysaccharides	1081:1109	Comprehensive Microarray Polymer Profiling (CoMPP) measured remaining extractable polysaccharides, showing that the enzyme-pretreatment was more successful at removing noncellulosic polysaccharides than alkaline- or acid-hydrolysis alone.
30525515	7	54	with	combination	1231:1241	arg1	pretreatment					1263:1274	a mild high-pH pretreatment	1248:1274	a mild high-pH pretreatment	1248:1274	While effective alone, the effect of the enzyme-pretreatment was bolstered via combination with a mild high-pH pretreatment.
30525515	5	55	attach	isolated	765:772	arg2	cellulose					748:756	cellulose	748:756	cellulose	748:756	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	5	55	attach	isolated	765:772	arg1	biomass					783:789	the biomass	779:789	the biomass	779:789	It is hypothesized that cellulose can be isolated from the biomass, using enzymes only, due to the low lignin content, facilitating greater liberation of CNF via high-shear homogenization.
30525515	1	56	theme	high-shear	155:164	arg1	homogenization					166:179	high-shear homogenization	155:179	high-shear homogenization	155:179	Cellulose fibers can be freed from the cell-wall skeleton via high-shear homogenization, to produce cellulose nanofibers (CNF) that can be used, for example, as the reinforcing phase in composite materials.
30525515	4	57	theme	sugar	656:660	arg1	beet					662:665	sugar beet	656:665	sugar beet	656:665	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	4	57	theme	sugar	656:660	arg1	byproducts					633:642	agro-industrial byproducts	617:642	agro-industrial byproducts (potato and sugar beet)	617:666	An enzyme-only pretreatment to obtain CNF from agro-industrial byproducts (potato and sugar beet) was developed with targeted commercial enzyme mixtures.
30525515	0	58	theme	Enzymatic	68:76	arg1	Pretreatments					78:90	Entirely Enzymatic Pretreatments	59:90	Entirely Enzymatic Pretreatments	59:90	Nanofibers Produced from Agro-Industrial Plant Waste Using Entirely Enzymatic Pretreatments.
30525515	9	59	theme	Enzyme-pretreated	1363:1379	arg1	suspensions					1381:1391	Enzyme-pretreated suspensions	1363:1391	Enzyme-pretreated suspensions	1363:1391	Enzyme-pretreated suspensions showed 4-fold and 10-fold increases in the storage modulus for potato and sugar beet, respectively, compared to untreated samples.
30525515	2	60	theme	harsh	371:375	arg1	chemical-pretreatments					377:398	harsh chemical-pretreatments	371:398	harsh chemical-pretreatments	371:398	Nanofiber production from agro-industrial byproducts normally involves harsh chemical-pretreatments and high temperatures to remove noncellulosic polysaccharides (20-70% of dry weight).
30525515	7	61	theme	high-pH	1255:1261	arg1	pretreatment					1263:1274	a mild high-pH pretreatment	1248:1274	a mild high-pH pretreatment	1248:1274	While effective alone, the effect of the enzyme-pretreatment was bolstered via combination with a mild high-pH pretreatment.
31521290	5	0	contain	contained	754:762	arg2	chain					803:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	5	0	contain	contained	754:762	arg1	FMG-Hm					747:752	FMG-Hm	747:752	FMG-Hm	747:752	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	3	1	theme	composition	490:500	arg1	analysis					459:466	An analysis	456:466	An analysis of the monosaccharide composition	456:500	An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17.
31521290	6	2	theme	predicted	907:915	arg1	structure					917:925	The predicted structure	903:925	The predicted structure of the heteropolysaccharide	903:953	The predicted structure of the heteropolysaccharide was established as.
31521290	4	3	theme	acid	682:685	arg1	hydrolysis					687:696	partial acid hydrolysis	674:696	partial acid hydrolysis	674:696	The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments.
31521290	1	4	theme	antimelanoma	252:263	arg1	effects					265:271	promising in vitro antimelanoma effects	233:271	promising in vitro antimelanoma effects	233:271	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	5	5	theme	α-L-fucopyranose	863:878	arg1	ends					855:858	non-reducing ends	842:858	non-reducing ends of α-L-fucopyranose and β-D-mannopyranose	842:900	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	4	6	theme	NMR	703:705	arg1	experiments					707:717	NMR experiments	703:717	NMR experiments	703:717	The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments.
31521290	5	7	theme	-linked	773:779	arg1	chain					803:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	1	8	theme	17.1 kDa	159:166	arg1	weight					149:154	a molecular weight	137:154	a molecular weight of 17.1 kDa	137:166	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	0	9	theme	Structure	0:8	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom	0:78	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	1	10	with	fucomannogalactan	104:120	arg1	weight					149:154	a molecular weight	137:154	a molecular weight of 17.1 kDa	137:166	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	5	11	theme	non-reducing	842:853	arg1	ends					855:858	non-reducing ends	842:858	non-reducing ends of α-L-fucopyranose and β-D-mannopyranose	842:900	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	2	12	theme	B16-F10	425:431	arg1	cells					449:453	B16-F10 murine melanoma cells	425:453	B16-F10 murine melanoma cells	425:453	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	0	13	dep	Hypsizygus	80:89	arg1	marmoreus					91:99	Hypsizygus marmoreus	80:99	Hypsizygus marmoreus	80:99	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	0	13	dep	Hypsizygus	80:89	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom	0:78	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	4	14	theme	methylation	652:662	arg1	analysis					664:671	methylation analysis	652:671	methylation analysis	652:671	The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments.
31521290	5	15	theme	main	798:801	arg1	chain					803:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	5	16	theme	galactopyranosyl	781:796	arg1	chain					803:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	2	17	theme	cell	407:410	arg1	migration					412:420	cell migration	407:420	cell migration	407:420	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	5	18	theme	β-D-mannopyranose	884:900	arg1	ends					855:858	non-reducing ends	842:858	non-reducing ends of α-L-fucopyranose and β-D-mannopyranose	842:900	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	0	19	theme	fucomannogalactan	37:53	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom	0:78	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	3	20	theme	monosaccharide	475:488	arg1	composition					490:500	the monosaccharide composition	471:500	the monosaccharide composition	471:500	An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17.
31521290	1	21	dep	in	243:244	arg1	vitro					246:250	vitro	246:250	vitro	246:250	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	0	22	theme	bioactive	27:35	arg1	fucomannogalactan					37:53	a bioactive fucomannogalactan	25:53	a bioactive fucomannogalactan	25:53	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	0	23	from	mushroom	71:78	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom	0:78	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	2	24	theme	colony-forming	380:393	arg1	ability					395:401	colony-forming ability	380:401	colony-forming ability	380:401	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	6	25	theme	heteropolysaccharide	934:953	arg1	structure					917:925	The predicted structure	903:925	The predicted structure of the heteropolysaccharide	903:953	The predicted structure of the heteropolysaccharide was established as.
31521290	2	26	theme	melanoma	440:447	arg1	cells					449:453	B16-F10 murine melanoma cells	425:453	B16-F10 murine melanoma cells	425:453	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	2	27	theme	cell	321:324	arg1	morphology					326:335	the cell morphology	317:335	the cell morphology	317:335	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	1	28	theme	marmoreus	213:221	arg1	bodies					192:197	fruiting bodies	183:197	fruiting bodies of Hypsizygus marmoreus	183:221	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	1	29	theme	molecular	139:147	arg1	weight					149:154	a molecular weight	137:154	a molecular weight of 17.1 kDa	137:166	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	5	30	link	-linked	773:779	arg1	chain					803:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	a α-(1→6)-linked galactopyranosyl main chain	764:807	The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose.
31521290	1	31	theme	fruiting	183:190	arg1	bodies					192:197	fruiting bodies	183:197	fruiting bodies of Hypsizygus marmoreus	183:221	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	4	32	theme	partial	674:680	arg1	hydrolysis					687:696	partial acid hydrolysis	674:696	partial acid hydrolysis	674:696	The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments.
31521290	1	33	theme	promising	233:241	arg1	effects					265:271	promising in vitro antimelanoma effects	233:271	promising in vitro antimelanoma effects	233:271	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31521290	0	34	theme	edible	64:69	arg1	mushroom					71:78	the edible mushroom	60:78	the edible mushroom	60:78	Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
31521290	3	35	theme	1.00:1.08:3.17	585:598	arg1	ratio					576:580	a ratio	574:580	a ratio of 1.00:1.08:3.17	574:598	An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17.
31521290	2	36	theme	murine	433:438	arg1	cells					449:453	B16-F10 murine melanoma cells	425:453	B16-F10 murine melanoma cells	425:453	FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells.
31521290	1	37	theme	in	243:244	arg1	effects					265:271	promising in vitro antimelanoma effects	233:271	promising in vitro antimelanoma effects	233:271	A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects.
31505207	5	0	theme	drug	930:933	arg1	molecules					935:943	the drug molecules	926:943	the drug molecules	926:943	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	7	1	theme	intestinal	1218:1227	arg1	pH 7.4					1236:1241	pH 7.4	1236:1241	pH 7.4	1236:1241	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	7	1	theme	intestinal	1218:1227	arg1	fluid					1229:1233	the intestinal fluid	1214:1233	the intestinal fluid (pH 7.4)	1214:1242	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	1	2	theme	composites	182:191	arg1	types					152:156	Two types	148:156	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	148:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	8	3	theme	mechanisms	1531:1540	arg1	combination					1494:1504	a combination	1492:1504	a combination of erosion and diffusion mechanisms	1492:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	0	4	theme	drug	92:95	arg1	studies					110:116	5-fluorouracil drug; equilibrium studies	77:116	5-fluorouracil drug; equilibrium studies	77:116	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	7	5	theme	complete	1187:1194	arg1	release					1196:1202	the complete release	1183:1202	the complete release	1183:1202	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	8	6	from	BE/CTAB	1460:1466	arg1	BE/HEMA-MMA					1408:1418	BE/HEMA-MMA	1408:1418	BE/HEMA-MMA	1408:1418	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	8	6	from	BE/CTAB	1460:1466	arg1	properties					1438:1447	the releasing properties	1424:1447	the releasing properties of it	1424:1453	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	6	7	theme	thermodynamic	950:962	arg1	parameters					964:973	The thermodynamic parameters	946:973	The thermodynamic parameters	946:973	The thermodynamic parameters reflected spontaneous loading reactions of endothermic nature.
31505207	7	8	theme	releasing	1042:1050	arg1	profile					1052:1058	The releasing profile	1038:1058	The releasing profile	1038:1058	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	0	9	theme	equilibrium	98:108	arg1	studies					110:116	5-fluorouracil drug; equilibrium studies	77:116	5-fluorouracil drug; equilibrium studies	77:116	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	8	10	theme	releasing	1428:1436	arg1	properties					1438:1447	the releasing properties	1424:1447	the releasing properties of it	1424:1453	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	3	11	theme	BE/CH	608:612	arg1	suitability					580:590	the suitability	576:590	the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively	576:693	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	0	12	theme	pharmacokinetic	122:136	arg1	behavior					138:145	pharmacokinetic behavior	122:145	pharmacokinetic behavior	122:145	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	7	13	theme	gastric	1251:1257	arg1	pH 1.2					1266:1271	pH 1.2	1266:1271	pH 1.2	1266:1271	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	7	13	theme	gastric	1251:1257	arg1	fluid					1259:1263	the gastric fluid	1247:1263	the gastric fluid (pH 1.2)	1247:1272	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	8	14	theme	it	1452:1453	arg1	BE/HEMA-MMA					1408:1418	BE/HEMA-MMA	1408:1418	BE/HEMA-MMA	1408:1418	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	8	14	theme	it	1452:1453	arg1	properties					1438:1447	the releasing properties	1424:1447	the releasing properties of it	1424:1453	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	2	15	theme	releasing	514:522	arg1	behavior					524:531	controlled releasing behavior	503:531	controlled releasing behavior	503:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	3	16	theme	BE/CTAB	599:605	arg1	suitability					580:590	the suitability	576:590	the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively	576:693	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	7	17	dep	formation	1100:1108	arg1	either					1204:1209	either	1204:1209	either	1204:1209	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	4	18	theme	loading	700:706	arg1	behaviors					708:716	The loading behaviors	696:716	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA	696:751	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	2	19	theme	controlled	503:512	arg1	behavior					524:531	controlled releasing behavior	503:531	controlled releasing behavior	503:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	3	20	theme	BE	595:596	arg1	suitability					580:590	the suitability	576:590	the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively	576:693	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	7	21	theme	BE/HEMA-MMA	1296:1306	arg1	composite					1308:1316	BE/HEMA-MMA composite	1296:1316	BE/HEMA-MMA composite	1296:1316	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	1	22	dep	composites	182:191	arg1	BE/CH					214:218	BE/CH	214:218	BE/CH	214:218	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	22	dep	composites	182:191	arg1	BE/HEMA-MMA					292:302	BE/HEMA-MMA	292:302	BE/HEMA-MMA	292:302	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	22	dep	composites	182:191	arg1	bentonite/chitosan					194:211	bentonite/chitosan	194:211	bentonite/chitosan (BE/CH)	194:219	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	22	dep	composites	182:191	arg1	composites					182:191	bentonite/biopolymer composites	161:191	bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	161:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	22	dep	composites	182:191	arg1	methacrylate					278:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate	225:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)	225:303	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	4	23	theme	BE/CTAB	721:727	arg1	behaviors					708:716	The loading behaviors	696:716	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA	696:751	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	0	24	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.	0:146	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	6	25	theme	loading	997:1003	arg1	reactions					1005:1013	spontaneous loading reactions	985:1013	spontaneous loading reactions of endothermic nature	985:1035	The thermodynamic parameters reflected spontaneous loading reactions of endothermic nature.
31505207	8	26	from	BE/HEMA-MMA	1408:1418	arg1	5FU					1399:1401	5FU	1399:1401	5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms	1399:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	3	27	theme	load	633:636	arg1	114 mg/g					638:645	load 114 mg/g	633:645	load 114 mg/g	633:645	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	2	28	theme	low-cost	426:433	arg1	carriers					435:442	low-cost carriers	426:442	low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior	426:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	2	28	theme	low-cost	426:433	arg1	products					395:402	The products	391:402	The products	391:402	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	0	29	theme	bentonite/biopolymer	20:39	arg1	composites					41:50	bentonite/biopolymer composites	20:50	bentonite/biopolymer composites	20:50	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	4	30	theme	Langmuir	787:794	arg1	model					796:800	the Langmuir model	783:800	the Langmuir model	783:800	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	2	31	theme	high	475:478	arg1	properties					488:497	high loading properties	475:497	high loading properties	475:497	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	2	32	theme	loading	480:486	arg1	properties					488:497	high loading properties	475:497	high loading properties	475:497	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	1	33	theme	bentonite	345:353	arg1	modification					329:340	modification	329:340	modification of bentonite	329:353	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	3	34	theme	BE/HEMA-MMA	618:628	arg1	suitability					580:590	the suitability	576:590	the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively	576:693	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	1	35	theme	bentonite/Co-Poly	225:241	arg1	BE/HEMA-MMA					292:302	BE/HEMA-MMA	292:302	BE/HEMA-MMA	292:302	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	35	theme	bentonite/Co-Poly	225:241	arg1	methacrylate					278:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate	225:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)	225:303	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	35	theme	bentonite/Co-Poly	225:241	arg1	composites					182:191	bentonite/biopolymer composites	161:191	bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	161:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	6	36	theme	nature	1030:1035	arg1	reactions					1005:1013	spontaneous loading reactions	985:1013	spontaneous loading reactions of endothermic nature	985:1035	The thermodynamic parameters reflected spontaneous loading reactions of endothermic nature.
31505207	5	37	theme	coulombic	893:901	arg1	mechanism					882:890	a physisorption mechanism	866:890	a physisorption mechanism (coulombic attractive forces) for the drug molecules	866:943	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	5	37	theme	coulombic	893:901	arg1	forces					914:919	coulombic attractive forces	893:919	coulombic attractive forces	893:919	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	8	38	theme	inspected	1323:1331	arg1	pharmacokinetics					1333:1348	The inspected pharmacokinetics	1319:1348	The inspected pharmacokinetics	1319:1348	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	1	39	theme	2-hydroxyethyl	243:256	arg1	BE/HEMA-MMA					292:302	BE/HEMA-MMA	292:302	BE/HEMA-MMA	292:302	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	39	theme	2-hydroxyethyl	243:256	arg1	methacrylate					278:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate	225:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)	225:303	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	39	theme	2-hydroxyethyl	243:256	arg1	composites					182:191	bentonite/biopolymer composites	161:191	bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	161:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	0	40	theme	composites	41:50	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.	0:146	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	6	41	theme	endothermic	1018:1028	arg1	nature					1030:1035	endothermic nature	1018:1035	endothermic nature	1018:1035	The thermodynamic parameters reflected spontaneous loading reactions of endothermic nature.
31505207	5	42	theme	adsorption	807:816	arg1	energies					818:825	The adsorption energies	803:825	The adsorption energies	803:825	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	2	43	theme	properties	488:497	arg1	drug					467:470	the 5-fluorouracil drug	448:470	the 5-fluorouracil drug of high loading properties and controlled releasing behavior	448:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	5	44	theme	attractive	903:912	arg1	mechanism					882:890	a physisorption mechanism	866:890	a physisorption mechanism (coulombic attractive forces) for the drug molecules	866:943	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	5	44	theme	attractive	903:912	arg1	forces					914:919	coulombic attractive forces	893:919	coulombic attractive forces	893:919	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	1	45	theme	methacrylate-methyl	258:276	arg1	BE/HEMA-MMA					292:302	BE/HEMA-MMA	292:302	BE/HEMA-MMA	292:302	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	45	theme	methacrylate-methyl	258:276	arg1	methacrylate					278:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate	225:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)	225:303	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	45	theme	methacrylate-methyl	258:276	arg1	composites					182:191	bentonite/biopolymer composites	161:191	bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	161:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	2	46	theme	behavior	524:531	arg1	drug					467:470	the 5-fluorouracil drug	448:470	the 5-fluorouracil drug of high loading properties and controlled releasing behavior	448:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	1	47	theme	organic	361:367	arg1	BE/CTAB					381:387	BE/CTAB	381:387	BE/CTAB	381:387	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	47	theme	organic	361:367	arg1	surfactant					369:378	an organic surfactant	358:378	an organic surfactant (BE/CTAB)	358:388	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	5	48	theme	thermodynamic	835:847	arg1	studies					849:855	the thermodynamic studies	831:855	the thermodynamic studies	831:855	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	0	49	theme	low-cost	55:62	arg1	carriers					64:71	low-cost carriers	55:71	low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior	55:145	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	4	50	theme	BE/HEMA-MMA	741:751	arg1	behaviors					708:716	The loading behaviors	696:716	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA	696:751	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	4	51	with	fitting	770:776	arg1	model					796:800	the Langmuir model	783:800	the Langmuir model	783:800	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	7	52	theme	bentonite/biopolymer	1113:1132	arg1	composites					1134:1143	bentonite/biopolymer composites	1113:1143	bentonite/biopolymer composites to extend for 160 h without attending the complete release	1113:1202	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	8	53	theme	erosion	1509:1515	arg1	combination					1494:1504	a combination	1492:1504	a combination of erosion and diffusion mechanisms	1492:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	3	54	theme	experimental	538:549	arg1	results					559:565	The experimental loading results	534:565	The experimental loading results	534:565	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	7	55	theme	composites	1134:1143	arg1	formation					1100:1108	the formation	1096:1108	the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release	1096:1202	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	7	56	theme	significant	1067:1077	arg1	enhancement					1079:1089	significant enhancement	1067:1089	significant enhancement	1067:1089	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
31505207	3	57	theme	loading	551:557	arg1	results					559:565	The experimental loading results	534:565	The experimental loading results	534:565	The experimental loading results revealed the suitability of BE, BE/CTAB, BE/CH and BE/HEMA-MMA to load 114 mg/g, 230 mg/g, 273 mg/g, and 310 mg/g, respectively.
31505207	8	58	from	properties	1438:1447	arg1	5FU					1399:1401	5FU	1399:1401	5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms	1399:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	4	59	theme	excellent	760:768	arg1	fitting					770:776	excellent fitting	760:776	excellent fitting	760:776	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	0	60	theme	5-fluorouracil	77:90	arg1	studies					110:116	5-fluorouracil drug; equilibrium studies	77:116	5-fluorouracil drug; equilibrium studies	77:116	Facile synthesis of bentonite/biopolymer composites as low-cost carriers for 5-fluorouracil drug; equilibrium studies and pharmacokinetic behavior.
31505207	2	61	theme	5-fluorouracil	452:465	arg1	drug					467:470	the 5-fluorouracil drug	448:470	the 5-fluorouracil drug of high loading properties and controlled releasing behavior	448:531	The products were characterized as low-cost carriers for the 5-fluorouracil drug of high loading properties and controlled releasing behavior.
31505207	8	62	theme	diffusion	1521:1529	arg1	mechanisms					1531:1540	diffusion mechanisms	1521:1540	diffusion mechanisms	1521:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	8	63	theme	erosion	1360:1366	arg1	mechanism					1368:1376	erosion mechanism	1360:1376	erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms	1360:1540	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	4	64	theme	BE/CH	730:734	arg1	behaviors					708:716	The loading behaviors	696:716	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA	696:751	The loading behaviors of BE/CTAB, BE/CH, and BE/HEMA-MMA are of excellent fitting with the Langmuir model.
31505207	6	65	theme	spontaneous	985:995	arg1	reactions					1005:1013	spontaneous loading reactions	985:1013	spontaneous loading reactions of endothermic nature	985:1035	The thermodynamic parameters reflected spontaneous loading reactions of endothermic nature.
31505207	5	66	theme	physisorption	868:880	arg1	mechanism					882:890	a physisorption mechanism	866:890	a physisorption mechanism (coulombic attractive forces) for the drug molecules	866:943	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	5	66	theme	physisorption	868:880	arg1	forces					914:919	coulombic attractive forces	893:919	coulombic attractive forces	893:919	The adsorption energies and the thermodynamic studies revealed a physisorption mechanism (coulombic attractive forces) for the drug molecules.
31505207	1	67	theme	bentonite/biopolymer	161:180	arg1	bentonite/chitosan					194:211	bentonite/chitosan	194:211	bentonite/chitosan (BE/CH)	194:219	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	67	theme	bentonite/biopolymer	161:180	arg1	composites					182:191	bentonite/biopolymer composites	161:191	bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA))	161:304	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	1	67	theme	bentonite/biopolymer	161:180	arg1	methacrylate					278:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate	225:289	bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)	225:303	Two types of bentonite/biopolymer composites (bentonite/chitosan (BE/CH) and bentonite/Co-Poly 2-hydroxyethyl methacrylate-methyl methacrylate (BE/HEMA-MMA)) were synthesized after modification of bentonite by an organic surfactant (BE/CTAB).
31505207	8	68	from	BE/CH	1472:1476	arg1	BE/HEMA-MMA					1408:1418	BE/HEMA-MMA	1408:1418	BE/HEMA-MMA	1408:1418	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	8	68	from	BE/CH	1472:1476	arg1	properties					1438:1447	the releasing properties	1424:1447	the releasing properties of it	1424:1453	The inspected pharmacokinetics reflected erosion mechanism for the releasing of 5FU from BE/HEMA-MMA and the releasing properties of it from BE/CTAB and BE/CH controlled by a combination of erosion and diffusion mechanisms.
31505207	7	69	dep	composites	1134:1143	arg1	extend					1148:1153	extend	1148:1153	to extend for 160 h without attending the complete release	1145:1202	The releasing profile showed significant enhancement with the formation of bentonite/biopolymer composites to extend for 160 h without attending the complete release either in the intestinal fluid (pH 7.4) or the gastric fluid (pH 1.2) with a preference for BE/HEMA-MMA composite.
30486344	8	0	theme	90	1307:1308	arg1	%					1302:1302	%	1302:1302	%	1302:1302	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	4	1	theme	asprella	580:587	arg1	samples					566:572	samples	566:572	samples of I. asprella	566:587	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	4	2	from	study	515:519	arg1	samples					566:572	samples	566:572	samples of I. asprella	566:587	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	4	2	from	study	515:519	arg1	evaluation					528:537	the evaluation	524:537	the evaluation of fungal contamination	524:561	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	7	3	theme	sterigmatocystin	1117:1132	arg1	polysaccharide					1094:1107	polysaccharide	1094:1107	polysaccharide	1094:1107	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	3	theme	sterigmatocystin	1117:1132	arg1	saponin					1085:1091	total saponin	1079:1091	total saponin	1079:1091	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	3	theme	sterigmatocystin	1117:1132	arg1	levels					1134:1139	the sterigmatocystin levels	1113:1139	the sterigmatocystin levels	1113:1139	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	3	theme	sterigmatocystin	1117:1132	arg1	indexes					1152:1158	quality indexes	1144:1158	quality indexes	1144:1158	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	0	4	theme	Producer	100:107	arg1	versicolor					121:130	the Sterigmatocystin Producer Aspergillus versicolor	79:130	the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	79:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	8	5	dep	%	1302:1302	arg1	to					1304:1305	to	1304:1305	to	1304:1305	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	3	6	theme	versicolor	387:396	arg1	sterigmatocystin					408:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	0	7	theme	Sterigmatocystin	83:98	arg1	versicolor					121:130	the Sterigmatocystin Producer Aspergillus versicolor	79:130	the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	79:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	6	8	theme	A.	914:915	arg1	versicolor					917:926	A. versicolor	914:926	A. versicolor	914:926	The herb, manually innoculated with A. versicolor, was stored under different temperatures (20⁻40 °C) and humidity (80⁻95%) for seven days.
30486344	9	9	with	environment	1494:1504	arg1	temperature					1524:1534	temperature	1524:1534	temperature	1524:1534	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	9	9	with	environment	1494:1504	arg1	humidity					1511:1518	humidity	1511:1518	humidity	1511:1518	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	6	10	theme	different	946:954	arg1	temperatures					956:967	different temperatures	946:967	different temperatures (20⁻40 °C)	946:978	The herb, manually innoculated with A. versicolor, was stored under different temperatures (20⁻40 °C) and humidity (80⁻95%) for seven days.
30486344	6	10	theme	different	946:954	arg1	°C					976:977	20⁻40 °C	970:977	20⁻40 °C	970:977	The herb, manually innoculated with A. versicolor, was stored under different temperatures (20⁻40 °C) and humidity (80⁻95%) for seven days.
30486344	1	11	theme	main	262:265	arg1	material					271:278	the main raw material	258:278	the main raw material of Canton herbal tea	258:299	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	7	12	theme	total	1079:1083	arg1	polysaccharide					1094:1107	polysaccharide	1094:1107	polysaccharide	1094:1107	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	12	theme	total	1079:1083	arg1	saponin					1085:1091	total saponin	1079:1091	total saponin	1079:1091	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	12	theme	total	1079:1083	arg1	levels					1134:1139	the sterigmatocystin levels	1113:1139	the sterigmatocystin levels	1113:1139	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	12	theme	total	1079:1083	arg1	indexes					1152:1158	quality indexes	1144:1158	quality indexes	1144:1158	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	4	13	theme	dominant	625:632	arg1	versicolor					606:615	A. versicolor	603:615	A. versicolor	603:615	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	4	13	theme	dominant	625:632	arg1	contaminant					634:644	the dominant contaminant	621:644	the dominant contaminant	621:644	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	1	14	theme	Ilex	180:183	arg1	asprella					185:192	Ilex asprella	180:192	Ilex asprella	180:192	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	0	15	theme	Aspergillus	109:119	arg1	versicolor					121:130	the Sterigmatocystin Producer Aspergillus versicolor	79:130	the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	79:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	5	16	theme	asprella	818:825	arg1	conditions					801:810	the optimal storage conditions	781:810	the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin	781:875	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	1	17	theme	asprella	185:192	arg1	herb					213:216	a commonly used herb	197:216	a commonly used herb in Southern China	197:234	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	1	17	theme	asprella	185:192	arg1	root					172:175	The root	168:175	The root of Ilex asprella	168:192	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	9	18	theme	sterigmatocystin	1606:1621	arg1	production					1623:1632	sterigmatocystin production	1606:1632	sterigmatocystin production	1606:1632	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	2	19	theme	I.	302:303	arg1	asprella					305:312	I. asprella	302:312	I. asprella	302:312	I. asprella is readily contaminated by mildew because of rich nutrients.
30486344	8	20	theme	sterigmatocystin	1242:1257	arg1	sterigmatocystin					1242:1257	sterigmatocystin	1242:1257	sterigmatocystin	1242:1257	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	8	20	theme	sterigmatocystin	1242:1257	arg1	amounts					1231:1237	large amounts	1225:1237	large amounts of sterigmatocystin on I. asprella	1225:1272	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	3	21	theme	Aspergillus	375:385	arg1	sterigmatocystin					408:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	1	22	theme	raw	267:269	arg1	material					271:278	the main raw material	258:278	the main raw material of Canton herbal tea	258:299	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	8	23	theme	26	1334:1335	arg1	°C.					1337:1339	°C.	1337:1339	°C.	1337:1339	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	5	24	theme	composite	734:742	arg1	design					744:749	central composite design	726:749	central composite design	726:749	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	2	25	theme	rich	359:362	arg1	nutrients					364:372	rich nutrients	359:372	rich nutrients	359:372	I. asprella is readily contaminated by mildew because of rich nutrients.
30486344	0	26	theme	Conditions	24:33	arg1	Optimization					0:11	Optimization	0:11	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.	0:166	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	4	27	theme	contamination	549:561	arg1	evaluation					528:537	the evaluation	524:537	the evaluation of fungal contamination	524:561	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	0	28	theme	Response	138:145	arg1	Methodology					155:165	Response Surface Methodology	138:165	Response Surface Methodology	138:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	9	29	theme	I.	1451:1452	arg1	samples					1463:1469	I. asprella samples	1451:1469	I. asprella samples	1451:1469	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	0	30	theme	Storage	16:22	arg1	Conditions					24:33	Storage Conditions	16:33	Storage Conditions	16:33	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	7	31	theme	quality	1144:1150	arg1	polysaccharide					1094:1107	polysaccharide	1094:1107	polysaccharide	1094:1107	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	31	theme	quality	1144:1150	arg1	saponin					1085:1091	total saponin	1079:1091	total saponin	1079:1091	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	31	theme	quality	1144:1150	arg1	levels					1134:1139	the sterigmatocystin levels	1113:1139	the sterigmatocystin levels	1113:1139	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	7	31	theme	quality	1144:1150	arg1	indexes					1152:1158	quality indexes	1144:1158	quality indexes	1144:1158	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	1	32	theme	used	208:211	arg1	root					172:175	The root	168:175	The root of Ilex asprella	168:192	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	1	32	theme	used	208:211	arg1	herb					213:216	a commonly used herb	197:216	a commonly used herb in Southern China	197:234	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	5	33	theme	optimal	785:791	arg1	conditions					801:810	the optimal storage conditions	781:810	the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin	781:875	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	1	34	theme	Canton	283:288	arg1	tea					297:299	Canton herbal tea	283:299	Canton herbal tea	283:299	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	7	35	theme	temperature	1033:1043	arg1	effects					1022:1028	The effects	1018:1028	The effects of temperature and humidity	1018:1056	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	9	36	theme	asprella	1454:1461	arg1	samples					1463:1469	I. asprella samples	1451:1469	I. asprella samples	1451:1469	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	5	37	theme	central	726:732	arg1	design					744:749	central composite design	726:749	central composite design	726:749	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	3	38	theme	common	444:449	arg1	molds					451:455	the most common molds	435:455	the most common molds that contaminate foodstuffs and medicinal herbs	435:503	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	5	39	theme	storage	793:799	arg1	conditions					801:810	the optimal storage conditions	781:810	the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin	781:875	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	1	40	theme	herbal	290:295	arg1	tea					297:299	Canton herbal tea	283:299	Canton herbal tea	283:299	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	0	41	theme	Surface	147:153	arg1	Methodology					155:165	Response Surface Methodology	138:165	Response Surface Methodology	138:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	8	42	theme	total	1352:1356	arg1	saponin					1358:1364	total saponin	1352:1364	total saponin	1352:1364	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	1	43	from	herb	213:216	arg1	China					230:234	Southern China	221:234	Southern China	221:234	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	3	44	theme	molds	451:455	arg1	molds					451:455	the most common molds	435:455	the most common molds that contaminate foodstuffs and medicinal herbs	435:503	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	3	44	theme	molds	451:455	arg1	one					428:430	one	428:430	one	428:430	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	0	45	theme	Herb	52:55	arg1	asprella					62:69	the Medicinal Herb Ilex asprella	38:69	the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	38:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	1	46	theme	tea	297:299	arg1	material					271:278	the main raw material	258:278	the main raw material of Canton herbal tea	258:299	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	8	47	theme	°C.	1337:1339	arg1	Meanwhile					1341:1349	26 °C. Meanwhile	1334:1349	26 °C. Meanwhile	1334:1349	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	8	48	theme	polysaccharide	1370:1383	arg1	amounts					1385:1391	polysaccharide amounts	1370:1391	polysaccharide amounts	1370:1391	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	3	49	theme	medicinal	489:497	arg1	herbs					499:503	medicinal herbs	489:503	medicinal herbs	489:503	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	0	50	theme	Medicinal	42:50	arg1	Herb					52:55	the Medicinal Herb	38:55	the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	38:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	4	51	theme	fungal	542:547	arg1	contamination					549:561	fungal contamination	542:561	fungal contamination	542:561	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	7	52	theme	humidity	1049:1056	arg1	effects					1022:1028	The effects	1018:1028	The effects of temperature and humidity	1018:1056	The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes.
30486344	8	53	from	amounts	1231:1237	arg1	asprella					1265:1272	asprella	1265:1272	asprella	1265:1272	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	5	54	theme	response	683:690	arg1	methodology					700:710	response surface methodology	683:710	response surface methodology combined with central composite design	683:749	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	1	55	theme	Southern	221:228	arg1	China					230:234	Southern China	221:234	Southern China	221:234	The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea.
30486344	5	56	theme	surface	692:698	arg1	methodology					700:710	response surface methodology	683:710	response surface methodology combined with central composite design	683:749	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	9	57	theme	A.	1581:1582	arg1	versicolor					1584:1593	A. versicolor growth and sterigmatocystin production	1581:1632	A. versicolor growth and sterigmatocystin production	1581:1632	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	4	58	theme	A.	603:604	arg1	versicolor					606:615	A. versicolor	603:615	A. versicolor	603:615	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	4	58	theme	A.	603:604	arg1	contaminant					634:644	the dominant contaminant	621:644	the dominant contaminant	621:644	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	0	59	theme	asprella	62:69	arg1	Optimization					0:11	Optimization	0:11	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.	0:166	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	5	60	theme	sterigmatocystin	860:875	arg1	contamination					843:855	the contamination	839:855	the contamination of sterigmatocystin	839:875	In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin.
30486344	9	61	dep	versicolor	1584:1593	arg1	production					1623:1632	sterigmatocystin production	1606:1632	sterigmatocystin production	1606:1632	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	9	61	dep	versicolor	1584:1593	arg1	growth					1595:1600	growth	1595:1600	growth	1595:1600	These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.
30486344	3	62	theme	producing	398:406	arg1	sterigmatocystin					408:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin	375:423	Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs.
30486344	8	63	theme	large	1225:1229	arg1	sterigmatocystin					1242:1257	sterigmatocystin	1242:1257	sterigmatocystin	1242:1257	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	8	63	theme	large	1225:1229	arg1	amounts					1231:1237	large amounts	1225:1237	large amounts of sterigmatocystin on I. asprella	1225:1272	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	0	64	theme	Ilex	57:60	arg1	asprella					62:69	the Medicinal Herb Ilex asprella	38:69	the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology	38:165	Optimization of Storage Conditions of the Medicinal Herb Ilex asprella against the Sterigmatocystin Producer Aspergillus versicolor Using Response Surface Methodology.
30486344	4	65	theme	Previous	506:513	arg1	study					515:519	Previous study	506:519	Previous study on the evaluation of fungal contamination on samples of I. asprella	506:587	Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant.
30486344	8	66	theme	A.	1185:1186	arg1	versicolor					1188:1197	A. versicolor	1185:1197	A. versicolor	1185:1197	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30486344	8	67	theme	85	1300:1301	arg1	%					1302:1302	%	1302:1302	%	1302:1302	The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly.
30707997	0	0	theme	biodegradation	76:89	arg1	process					91:97	biodegradation process	76:97	biodegradation process	76:97	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	3	1	theme	Scanning	692:699	arg1	Microscopy					710:719	Scanning Electron Microscopy	692:719	Scanning Electron Microscopy (SEM)	692:725	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	1	theme	Scanning	692:699	arg1	SEM					722:724	SEM	722:724	SEM	722:724	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	5	2	theme	anhydrous	873:881	arg1	MA					895:896	MA	895:896	MA	895:896	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	2	theme	anhydrous	873:881	arg1	acid					889:892	anhydrous malic acid	873:892	anhydrous malic acid (MA)	873:897	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	3	from	increase	934:941	arg1	film					988:991	The TPS/PLA composite film	966:991	The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization	966:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	3	from	increase	934:941	arg1	index					959:963	the carbonyl index	946:963	the carbonyl index	946:963	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	1	4	theme	environmentally	235:249	arg1	products					205:212	petroleum products	195:212	petroleum products	195:212	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	4	theme	environmentally	235:249	arg1	materials					260:268	biodegradable and environmentally friendly materials	217:268	biodegradable and environmentally friendly materials	217:268	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	4	5	theme	carbonyl	837:844	arg1	index					846:850	the carbonyl index	833:850	the carbonyl index	833:850	The addition of PLA increases the carbonyl index.
30707997	5	6	theme	malic	883:887	arg1	MA					895:896	MA	895:896	MA	895:896	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	6	theme	malic	883:887	arg1	acid					889:892	anhydrous malic acid	873:892	anhydrous malic acid (MA)	873:897	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	1	7	theme	friendly	251:258	arg1	products					205:212	petroleum products	195:212	petroleum products	195:212	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	7	theme	friendly	251:258	arg1	materials					260:268	biodegradable and environmentally friendly materials	217:268	biodegradable and environmentally friendly materials	217:268	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	6	8	theme	film	1119:1122	arg1	size					1124:1127	film size	1119:1127	film size	1119:1127	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	5	9	theme	biodegradation	1027:1040	arg1	disintegration					1043:1056	disintegration	1043:1056	disintegration	1043:1056	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	9	theme	biodegradation	1027:1040	arg1	phases					1017:1022	the three phases	1007:1022	the three phases of biodegradation: disintegration, fragmentation, and mineralization	1007:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	9	theme	biodegradation	1027:1040	arg1	fragmentation					1059:1071	fragmentation	1059:1071	fragmentation	1059:1071	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	9	theme	biodegradation	1027:1040	arg1	mineralization					1078:1091	mineralization	1078:1091	mineralization	1078:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	3	10	from	changes	767:773	arg1	film					782:785	the film	778:785	the film	778:785	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	5	11	from	introduction	857:868	arg1	mixtures					910:917	PLA/TPS mixtures	902:917	PLA/TPS mixtures	902:917	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	6	12	from	reduction	1106:1114	arg1	size					1124:1127	film size	1119:1127	film size	1119:1127	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	4	13	theme	PLA	819:821	arg1	addition					807:814	The addition	803:814	The addition of PLA	803:821	The addition of PLA increases the carbonyl index.
30707997	3	14	theme	Electron	701:708	arg1	Microscopy					710:719	Scanning Electron Microscopy	692:719	Scanning Electron Microscopy (SEM)	692:725	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	14	theme	Electron	701:708	arg1	SEM					722:724	SEM	722:724	SEM	722:724	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	15	theme	Microscopy	710:719	arg1	tests					727:731	High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests	646:731	High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests	646:731	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	6	16	with	thinning	1149:1156	arg1	fractures					1175:1183	fractures	1175:1183	fractures that produced fragmentation and disintegration	1175:1230	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	3	17	theme	Infrared	567:574	arg1	Spectroscopy					576:587	Infrared Spectroscopy	567:587	Infrared Spectroscopy	567:587	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	18	from	groups	626:631	arg1	film					636:639	film	636:639	film	636:639	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	0	19	theme	Structural	0:9	arg1	changes					11:17	Structural changes	0:17	Structural changes of cassava starch and polylactic acid films	0:61	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	6	20	located	observed	1133:1140	arg1	week					1097:1100	week 4	1097:1102	week 4	1097:1102	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	6	20	located	observed	1133:1140	arg2	reduction					1106:1114	a reduction	1104:1114	a reduction in film size	1104:1127	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	6	21	theme	film	1165:1168	arg1	thinning					1149:1156	a thinning	1147:1156	a thinning of the film with fractures that produced fragmentation and disintegration	1147:1230	In week 4 a reduction in film size was observed with a thinning of the film with fractures that produced fragmentation and disintegration.
30707997	5	22	theme	PLA/TPS	902:908	arg1	mixtures					910:917	PLA/TPS mixtures	902:917	PLA/TPS mixtures	902:917	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	0	23	theme	cassava	22:28	arg1	starch					30:35	cassava starch	22:35	cassava starch	22:35	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	3	24	theme	Fourier	549:555	arg1	tests					597:601	Fourier Transform Infrared Spectroscopy (FT-IR) tests	549:601	Fourier Transform Infrared Spectroscopy (FT-IR) tests	549:601	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	1	25	theme	packaging	174:182	arg1	manufacture					159:169	the manufacture	155:169	the manufacture of packaging	155:182	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	2	26	theme	biodegradation	401:414	arg1	process					416:422	a biodegradation process	399:422	a biodegradation process	399:422	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	2	27	theme	ISO	477:479	arg1	guidelines					463:472	the guidelines	459:472	the guidelines of ISO 4855-2:2007	459:491	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	0	28	theme	starch	30:35	arg1	changes					11:17	Structural changes	0:17	Structural changes of cassava starch and polylactic acid films	0:61	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	3	29	theme	Structural	756:765	arg1	changes					767:773	Structural changes	756:773	Structural changes in the film	756:785	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	2	30	theme	compost	430:436	arg1	conditions					438:447	compost conditions	430:447	compost conditions following the guidelines of ISO 4855-2:2007	430:491	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	1	31	theme	petroleum	195:203	arg1	products					205:212	petroleum products	195:212	petroleum products	195:212	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	31	theme	petroleum	195:203	arg1	materials					260:268	biodegradable and environmentally friendly materials	217:268	biodegradable and environmentally friendly materials	217:268	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	0	32	theme	acid	52:55	arg1	films					57:61	polylactic acid films	41:61	polylactic acid films	41:61	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	5	33	theme	TPS/PLA	970:976	arg1	film					988:991	The TPS/PLA composite film	966:991	The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization	966:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	33	theme	TPS/PLA	970:976	arg1	index					959:963	the carbonyl index	946:963	the carbonyl index	946:963	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	34	theme	acid	889:892	arg1	introduction					857:868	The introduction	853:868	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures	853:917	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	0	35	theme	polylactic	41:50	arg1	films					57:61	polylactic acid films	41:61	polylactic acid films	41:61	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	3	36	theme	Microscopy	670:679	arg1	tests					727:731	High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests	646:731	High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests	646:731	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	5	37	theme	composite	978:986	arg1	film					988:991	The TPS/PLA composite film	966:991	The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization	966:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	37	theme	composite	978:986	arg1	index					959:963	the carbonyl index	946:963	the carbonyl index	946:963	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	2	38	dep	structure	296:304	arg1	the					292:294	the	292:294	the	292:294	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	3	39	theme	functional	615:624	arg1	groups					626:631	functional groups	615:631	functional groups on film	615:639	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	2	40	theme	film	323:326	arg1	surface					310:316	surface	310:316	surface	310:316	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	2	40	theme	film	323:326	arg1	structure					296:304	structure	296:304	structure	296:304	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
30707997	1	41	theme	Polylactic	100:109	arg1	compounds					137:145	compounds	137:145	compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials	137:268	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	41	theme	Polylactic	100:109	arg1	PLA					117:119	PLA	117:119	PLA	117:119	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	41	theme	Polylactic	100:109	arg1	starch					126:131	starch	126:131	starch	126:131	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	41	theme	Polylactic	100:109	arg1	acid					111:114	Polylactic acid	100:114	Polylactic acid (PLA)	100:120	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	5	42	dep	phases	1017:1022	arg1	disintegration					1043:1056	disintegration	1043:1056	disintegration	1043:1056	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	42	dep	phases	1017:1022	arg1	phases					1017:1022	the three phases	1007:1022	the three phases of biodegradation: disintegration, fragmentation, and mineralization	1007:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	42	dep	phases	1017:1022	arg1	fragmentation					1059:1071	fragmentation	1059:1071	fragmentation	1059:1071	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	42	dep	phases	1017:1022	arg1	mineralization					1078:1091	mineralization	1078:1091	mineralization	1078:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	3	43	theme	High-Resolution	646:660	arg1	HROM					682:685	HROM	682:685	HROM	682:685	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	43	theme	High-Resolution	646:660	arg1	Microscopy					670:679	High-Resolution Optical Microscopy	646:679	High-Resolution Optical Microscopy (HROM)	646:686	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	44	theme	Optical	662:668	arg1	HROM					682:685	HROM	682:685	HROM	682:685	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	3	44	theme	Optical	662:668	arg1	Microscopy					670:679	High-Resolution Optical Microscopy	646:679	High-Resolution Optical Microscopy (HROM)	646:686	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	5	45	theme	carbonyl	950:957	arg1	film					988:991	The TPS/PLA composite film	966:991	The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization	966:1091	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	5	45	theme	carbonyl	950:957	arg1	index					959:963	the carbonyl index	946:963	the carbonyl index	946:963	The introduction of anhydrous malic acid (MA) in PLA/TPS mixtures may lead to an increase in the carbonyl index, The TPS/PLA composite film was framed in the three phases of biodegradation: disintegration, fragmentation, and mineralization.
30707997	3	46	dep	Fourier	549:555	arg1	Transform					557:565	Transform	557:565	Transform Infrared Spectroscopy	557:587	Samples were taken every week for one month to perform Fourier Transform Infrared Spectroscopy (FT-IR) tests to identify functional groups on film, and High-Resolution Optical Microscopy (HROM) and Scanning Electron Microscopy (SEM) tests, from these techniques Structural changes in the film were evidenced.
30707997	1	47	theme	biodegradable	217:229	arg1	products					205:212	petroleum products	195:212	petroleum products	195:212	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	1	47	theme	biodegradable	217:229	arg1	materials					260:268	biodegradable and environmentally friendly materials	217:268	biodegradable and environmentally friendly materials	217:268	Polylactic acid (PLA) and starch are compounds used in the manufacture of packaging to replace petroleum products as biodegradable and environmentally friendly materials.
30707997	0	48	theme	films	57:61	arg1	changes					11:17	Structural changes	0:17	Structural changes of cassava starch and polylactic acid films	0:61	Structural changes of cassava starch and polylactic acid films submitted to biodegradation process.
30707997	2	49	theme	cassava	359:365	arg1	starch					367:372	cassava starch	359:372	cassava starch	359:372	This study evaluated the structure and surface of a film manufactured by extrusion from cassava starch and PLA, which underwent a biodegradation process under compost conditions following the guidelines of ISO 4855-2:2007.
31398611	3	0	theme	surface	720:726	arg1	area					728:731	higher surface area	713:731	higher surface area	713:731	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	1	1	theme	composite	197:205	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	1	theme	composite	197:205	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	3	2	theme	total	737:741	arg1	volume					748:753	total pore volume	737:753	total pore volume	737:753	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	1	3	theme	A	288:288	arg1	removal					267:273	the removal	263:273	the removal of bisphenol A (BPA)	263:294	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	5	4	theme	isotherm	1150:1157	arg1	model					1159:1163	the Freundlich isotherm model	1135:1163	the Freundlich isotherm model	1135:1163	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	5	5	theme	pseudo-second-order	1087:1105	arg1	model					1116:1120	the pseudo-second-order kinetics model	1083:1120	the pseudo-second-order kinetics model	1083:1120	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	8	6	theme	high	1525:1528	arg1	adsorption					1530:1539	the high adsorption	1521:1539	the high adsorption	1521:1539	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	7	7	theme	adsorption	1486:1495	arg1	stacking					1413:1420	the π-π stacking	1405:1420	the π-π stacking	1405:1420	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	7	theme	adsorption	1486:1495	arg1	bonding					1432:1438	hydrogen bonding	1423:1438	hydrogen bonding	1423:1438	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	7	theme	adsorption	1486:1495	arg1	interaction					1453:1463	cation-π interaction	1444:1463	cation-π interaction	1444:1463	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	7	theme	adsorption	1486:1495	arg1	mechanisms					1497:1506	the primary adsorption mechanisms	1474:1506	the primary adsorption mechanisms	1474:1506	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	5	8	theme	Freundlich	1139:1148	arg1	model					1159:1163	the Freundlich isotherm model	1135:1163	the Freundlich isotherm model	1135:1163	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	7	9	theme	π-π	1409:1411	arg1	stacking					1413:1420	the π-π stacking	1405:1420	the π-π stacking	1405:1420	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	9	theme	π-π	1409:1411	arg1	bonding					1432:1438	hydrogen bonding	1423:1438	hydrogen bonding	1423:1438	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	9	theme	π-π	1409:1411	arg1	interaction					1453:1463	cation-π interaction	1444:1463	cation-π interaction	1444:1463	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	9	theme	π-π	1409:1411	arg1	mechanisms					1497:1506	the primary adsorption mechanisms	1474:1506	the primary adsorption mechanisms	1474:1506	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	6	10	theme	adsorption	1260:1269	arg1	efficiency					1271:1280	the adsorption efficiency	1256:1280	the adsorption efficiency	1256:1280	Importantly, the composite beads could be easily recycled and regenerated by methanol and the adsorption efficiency still maintained as high as 96% even after five cycles.
31398611	0	11	theme	composite	101:109	arg1	beads					111:115	composite beads	101:115	composite beads	101:115	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	0	12	from	solution	45:52	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium	0:81	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	7	13	theme	cation-π	1444:1451	arg1	stacking					1413:1420	the π-π stacking	1405:1420	the π-π stacking	1405:1420	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	13	theme	cation-π	1444:1451	arg1	bonding					1432:1438	hydrogen bonding	1423:1438	hydrogen bonding	1423:1438	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	13	theme	cation-π	1444:1451	arg1	interaction					1453:1463	cation-π interaction	1444:1463	cation-π interaction	1444:1463	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	13	theme	cation-π	1444:1451	arg1	mechanisms					1497:1506	the primary adsorption mechanisms	1474:1506	the primary adsorption mechanisms	1474:1506	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	2	14	theme	infrared	367:374	arg1	FTIR					390:393	FTIR	390:393	FTIR	390:393	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	14	theme	infrared	367:374	arg1	spectroscopy					376:387	infrared spectroscopy	367:387	infrared spectroscopy (FTIR)	367:394	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	4	15	theme	Al-MOF/SA	980:988	arg1	ones					990:993	the Al-MOF/SA ones	976:993	the Al-MOF/SA ones	976:993	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	3	16	theme	pore	743:746	arg1	volume					748:753	total pore volume	737:753	total pore volume	737:753	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	2	17	theme	Several	297:303	arg1	methods					305:311	Several methods	297:311	Several methods	297:311	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	17	theme	Several	297:303	arg1	diffraction					330:340	X-ray diffraction	324:340	X-ray diffraction (XRD)	324:346	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	8	18	theme	contaminated	1720:1731	arg1	water					1733:1737	contaminated water	1720:1737	contaminated water	1720:1737	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	2	19	theme	scanning	397:404	arg1	microscope					415:424	scanning electron microscope	397:424	scanning electron microscope (SEM)	397:430	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	19	theme	scanning	397:404	arg1	SEM					427:429	SEM	427:429	SEM	427:429	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	8	20	theme	water	1558:1562	arg1	stability					1564:1572	good water stability	1553:1572	good water stability	1553:1572	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	4	21	theme	adsorption	842:851	arg1	performance					853:863	the adsorption performance	838:863	the adsorption performance	838:863	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	0	22	theme	Enhanced	0:7	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium	0:81	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	2	23	theme	adsorption-desorption	442:462	arg1	isotherms					464:472	nitrogen adsorption-desorption isotherms	433:472	nitrogen adsorption-desorption isotherms	433:472	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	8	24	theme	good	1553:1556	arg1	stability					1564:1572	good water stability	1553:1572	good water stability	1553:1572	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	6	25	theme	composite	1183:1191	arg1	beads					1193:1197	the composite beads	1179:1197	the composite beads	1179:1197	Importantly, the composite beads could be easily recycled and regenerated by methanol and the adsorption efficiency still maintained as high as 96% even after five cycles.
31398611	2	26	theme	nitrogen	433:440	arg1	isotherms					464:472	nitrogen adsorption-desorption isotherms	433:472	nitrogen adsorption-desorption isotherms	433:472	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	5	27	theme	adsorption	1047:1056	arg1	process					1058:1064	the adsorption process	1043:1064	the adsorption process	1043:1064	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	0	28	theme	bisphenol	20:28	arg1	A					30:30	bisphenol A	20:30	bisphenol A	20:30	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	2	29	theme	X-ray	324:328	arg1	XRD					343:345	XRD	343:345	XRD	343:345	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	29	theme	X-ray	324:328	arg1	Fourier					349:355	Fourier	349:355	Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS)	349:515	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	29	theme	X-ray	324:328	arg1	diffraction					330:340	X-ray diffraction	324:340	X-ray diffraction (XRD)	324:346	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	5	30	theme	fitting	1022:1028	arg1	results					1030:1036	the fitting results	1018:1036	the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model	1018:1163	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	8	31	theme	easy	1586:1589	arg1	separation					1591:1600	especially easy separation	1575:1600	especially easy separation	1575:1600	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	2	32	theme	prepared	548:555	arg1	adsorbents					557:566	the prepared adsorbents	544:566	the prepared adsorbents	544:566	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	3	33	theme	beads	651:655	arg1	porosity					635:642	the porosity	631:642	the porosity of the beads	631:655	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	1	34	theme	Aluminum-based	118:131	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	34	theme	Aluminum-based	118:131	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	3	35	contain	possessed	703:711	arg2	volume					748:753	total pore volume	737:753	total pore volume	737:753	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	3	35	contain	possessed	703:711	arg1	beads					697:701	the obtained Al-MOF/SA-CS composite beads	661:701	the obtained Al-MOF/SA-CS composite beads	661:701	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	3	35	contain	possessed	703:711	arg2	area					728:731	higher surface area	713:731	higher surface area	713:731	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	2	36	theme	photoelectron	484:496	arg1	XPS					512:514	XPS	512:514	XPS	512:514	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	36	theme	photoelectron	484:496	arg1	spectroscopy					498:509	X-ray photoelectron spectroscopy	478:509	X-ray photoelectron spectroscopy (XPS)	478:515	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	8	37	theme	BPA	1711:1713	arg1	removal					1700:1706	the removal	1696:1706	the removal of BPA from contaminated water	1696:1737	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	4	38	theme	Al-MOF/SA-CS	945:956	arg1	beads					958:962	Al-MOF/SA-CS beads	945:962	Al-MOF/SA-CS beads	945:962	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	0	39	theme	A	30:30	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium	0:81	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	7	40	theme	primary	1478:1484	arg1	stacking					1413:1420	the π-π stacking	1405:1420	the π-π stacking	1405:1420	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	40	theme	primary	1478:1484	arg1	bonding					1432:1438	hydrogen bonding	1423:1438	hydrogen bonding	1423:1438	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	40	theme	primary	1478:1484	arg1	interaction					1453:1463	cation-π interaction	1444:1463	cation-π interaction	1444:1463	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	40	theme	primary	1478:1484	arg1	mechanisms					1497:1506	the primary adsorption mechanisms	1474:1506	the primary adsorption mechanisms	1474:1506	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	2	41	theme	X-ray	478:482	arg1	XPS					512:514	XPS	512:514	XPS	512:514	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	41	theme	X-ray	478:482	arg1	spectroscopy					498:509	X-ray photoelectron spectroscopy	478:509	X-ray photoelectron spectroscopy (XPS)	478:515	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	42	used	used	523:526	arg2	diffraction					330:340	X-ray diffraction	324:340	X-ray diffraction (XRD)	324:346	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	42	used	used	523:526	arg2	methods					305:311	Several methods	297:311	Several methods	297:311	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	3	43	theme	CS	615:616	arg1	introduction					599:610	the introduction	595:610	the introduction of CS	595:616	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	2	44	theme	electron	406:413	arg1	microscope					415:424	scanning electron microscope	397:424	scanning electron microscope (SEM)	397:430	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	2	44	theme	electron	406:413	arg1	SEM					427:429	SEM	427:429	SEM	427:429	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	4	45	theme	adsorption	909:918	arg1	capacity					920:927	the enhanced adsorption capacity	896:927	the enhanced adsorption capacity	896:927	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	5	46	theme	kinetics	1107:1114	arg1	model					1116:1120	the pseudo-second-order kinetics model	1083:1120	the pseudo-second-order kinetics model	1083:1120	It is also found from the fitting results that the adsorption process fitted well with the pseudo-second-order kinetics model and followed the Freundlich isotherm model.
31398611	8	47	from	water	1733:1737	arg1	removal					1700:1706	the removal	1696:1706	the removal of BPA from contaminated water	1696:1737	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	1	48	theme	metal-organic	133:145	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	48	theme	metal-organic	133:145	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	0	49	theme	aqueous	37:43	arg1	solution					45:52	aqueous solution	37:52	aqueous solution	37:52	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	8	50	theme	excellent	1607:1615	arg1	recyclability					1617:1629	excellent recyclability	1607:1629	excellent recyclability	1607:1629	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	4	51	theme	enhanced	900:907	arg1	capacity					920:927	the enhanced adsorption capacity	896:927	the enhanced adsorption capacity	896:927	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	3	52	theme	Al-MOF/SA-CS	674:685	arg1	beads					697:701	the obtained Al-MOF/SA-CS composite beads	661:701	the obtained Al-MOF/SA-CS composite beads	661:701	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	7	53	theme	experimental	1379:1390	arg1	results					1392:1398	the experimental results	1375:1398	the experimental results	1375:1398	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	1	54	theme	framework/sodium	147:162	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	54	theme	framework/sodium	147:162	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	8	55	dep	adsorption	1530:1539	arg1	properties					1541:1550	properties	1541:1550	properties	1541:1550	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	3	56	theme	composite	687:695	arg1	beads					697:701	the obtained Al-MOF/SA-CS composite beads	661:701	the obtained Al-MOF/SA-CS composite beads	661:701	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	7	57	theme	hydrogen	1423:1430	arg1	stacking					1413:1420	the π-π stacking	1405:1420	the π-π stacking	1405:1420	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	57	theme	hydrogen	1423:1430	arg1	bonding					1432:1438	hydrogen bonding	1423:1438	hydrogen bonding	1423:1438	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	57	theme	hydrogen	1423:1430	arg1	interaction					1453:1463	cation-π interaction	1444:1463	cation-π interaction	1444:1463	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	7	57	theme	hydrogen	1423:1430	arg1	mechanisms					1497:1506	the primary adsorption mechanisms	1474:1506	the primary adsorption mechanisms	1474:1506	Furthermore, it can be inferred from the experimental results that the π-π stacking, hydrogen bonding and cation-π interaction could be the primary adsorption mechanisms.
31398611	1	58	theme	alginate-chitosan	164:180	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	58	theme	alginate-chitosan	164:180	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	2	59	dep	Fourier	349:355	arg1	transform					357:365	transform	357:365	transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS)	357:515	Several methods, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), nitrogen adsorption-desorption isotherms and X-ray photoelectron spectroscopy (XPS), were used to characterize the prepared adsorbents.
31398611	4	60	theme	Batch	792:796	arg1	experiments					798:808	Batch experiments	792:808	Batch experiments	792:808	Batch experiments were carried out to explore the adsorption performance and the results displayed that the enhanced adsorption capacity was achieved by Al-MOF/SA-CS beads compared to the Al-MOF/SA ones.
31398611	0	61	theme	aluminum-based	57:70	arg1	MOF/sodium					72:81	aluminum-based MOF/sodium	57:81	aluminum-based MOF/sodium	57:81	Enhanced removal of bisphenol A from aqueous solution by aluminum-based MOF/sodium alginate-chitosan composite beads.
31398611	3	62	theme	obtained	665:672	arg1	beads					697:701	the obtained Al-MOF/SA-CS composite beads	661:701	the obtained Al-MOF/SA-CS composite beads	661:701	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	1	63	theme	Al-MOF/SA-CS	183:194	arg1	adsorbent					249:257	adsorbent	249:257	adsorbent	249:257	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	63	theme	Al-MOF/SA-CS	183:194	arg1	beads					207:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads	118:211	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	8	64	theme	composite	1645:1653	arg1	adsorbent					1682:1690	adsorbent	1682:1690	adsorbent	1682:1690	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	8	64	theme	composite	1645:1653	arg1	beads					1655:1659	Al-MOF/SA-CS composite beads	1632:1659	Al-MOF/SA-CS composite beads	1632:1659	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	3	65	theme	higher	713:718	arg1	area					728:731	higher surface area	713:731	higher surface area	713:731	Results demonstrated that the introduction of CS could change the porosity of the beads and the obtained Al-MOF/SA-CS composite beads possessed higher surface area and total pore volume comparing with the beads without CS.
31398611	8	66	theme	Al-MOF/SA-CS	1632:1643	arg1	adsorbent					1682:1690	adsorbent	1682:1690	adsorbent	1682:1690	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	8	66	theme	Al-MOF/SA-CS	1632:1643	arg1	beads					1655:1659	Al-MOF/SA-CS composite beads	1632:1659	Al-MOF/SA-CS composite beads	1632:1659	Considering the high adsorption properties, good water stability, especially easy separation, and excellent recyclability, Al-MOF/SA-CS composite beads could be a promising adsorbent for the removal of BPA from contaminated water.
31398611	1	67	theme	bisphenol	278:286	arg1	BPA					291:293	BPA	291:293	BPA	291:293	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
31398611	1	67	theme	bisphenol	278:286	arg1	A					288:288	bisphenol A	278:288	bisphenol A (BPA)	278:294	Aluminum-based metal-organic framework/sodium alginate-chitosan (Al-MOF/SA-CS) composite beads were synthesized and employed as an adsorbent for the removal of bisphenol A (BPA).
30047261	0	0	theme	Grafted	96:102	arg1	Interface					140:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	2	1	theme	corresponding	474:486	arg1	nanocomposites					488:501	the corresponding nanocomposites	470:501	the corresponding nanocomposites	470:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	7	2	dep	interface	1156:1164	arg1	effects					1198:1204	distribution effects	1185:1204	distribution effects	1185:1204	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	10	3	theme	modification	1498:1509	arg1	versatile					1542:1550	versatile	1542:1550	versatile	1542:1550	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	3	theme	modification	1498:1509	arg1	strategies					1527:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	4	theme	present	1490:1496	arg1	versatile					1542:1550	versatile	1542:1550	versatile	1542:1550	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	4	theme	present	1490:1496	arg1	strategies					1527:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	5	5	theme	CNF/PMMA	745:752	arg1	nanocomposites					754:767	CNF/PMMA nanocomposites	745:767	CNF/PMMA nanocomposites	745:767	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	9	6	theme	optical	1387:1393	arg1	transparency					1395:1406	much higher optical transparency	1375:1406	much higher optical transparency	1375:1406	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	7	7	theme	strong	1149:1154	arg1	interface					1156:1164	a strong interface	1147:1164	a strong interface	1147:1164	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	0	8	from	Effects	15:21	arg1	Content					41:47	High Cellulose Content	26:47	High Cellulose Content	26:47	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	9	9	theme	hygro-mechanical	1432:1447	arg1	properties					1449:1458	hygro-mechanical properties	1432:1458	hygro-mechanical properties	1432:1458	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	1	10	theme	materials	172:180	arg1	design					182:187	materials design	172:187	materials design of polymer nanocomposites	172:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	10	11	theme	cellulose	1572:1580	arg1	functionalities					1651:1665	functionalities	1651:1665	functionalities	1651:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	11	theme	cellulose	1572:1580	arg1	architectures					1620:1632	architectures	1620:1632	architectures	1620:1632	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	11	theme	cellulose	1572:1580	arg1	nanocomposites					1582:1595	cellulose nanocomposites	1572:1595	cellulose nanocomposites of other compositions, architectures, properties, and functionalities	1572:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	11	theme	cellulose	1572:1580	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	11	theme	cellulose	1572:1580	arg1	properties					1635:1644	properties	1635:1644	properties	1635:1644	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	2	12	theme	manipulation	313:324	arg1	effect					303:308	the effect	299:308	the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites	299:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	3	13	theme	changes	556:562	arg1	minimum					545:551	a minimum	543:551	a minimum of changes in material composition	543:586	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	0	14	theme	Cellulose-Poly	104:117	arg1	Interface					140:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	7	15	theme	distribution	1185:1196	arg1	effects					1198:1204	distribution effects	1185:1204	distribution effects	1185:1204	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	10	16	theme	other	1600:1604	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	6	17	theme	PMMA	963:966	arg1	grafts					968:973	PMMA grafts	963:973	PMMA grafts	963:973	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	0	18	theme	methacrylate	126:137	arg1	Interface					140:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	5	19	theme	CNF	853:855	arg1	mat					857:859	a porous CNF mat	844:859	a porous CNF mat	844:859	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	1	20	from	aspect	162:167	arg1	design					182:187	materials design	172:187	materials design of polymer nanocomposites	172:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	9	21	theme	thermal	1409:1415	arg1	stability					1417:1425	thermal stability	1409:1425	thermal stability	1409:1425	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	5	22	theme	porous	846:851	arg1	mat					857:859	a porous CNF mat	844:859	a porous CNF mat	844:859	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	4	23	theme	CNF	660:662	arg1	fibrils					664:670	CNF fibrils	660:670	CNF fibrils	660:670	The hydroxyl-rich surface of CNF fibrils is exploited to modify the CNF surface via an epoxide-hydroxyl reaction.
30047261	0	24	theme	methyl	119:124	arg1	Interface					140:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface	83:148	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	8	25	theme	relative	1275:1282	arg1	conditions					1293:1302	different relative humidity conditions	1265:1302	different relative humidity conditions	1265:1302	Moisture uptake and mechanical properties are measured at different relative humidity conditions.
30047261	7	26	theme	high	1092:1095	arg1	content					1097:1103	The high content	1088:1103	The high content of CNF fibrils of ∼6 nm diameter	1088:1136	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	2	27	theme	thermal	432:438	arg1	properties					456:465	the optical, thermal, and mechanical properties	419:465	the optical, thermal, and mechanical properties of the corresponding nanocomposites	419:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	7	28	theme	diameter	1129:1136	arg1	fibrils					1112:1118	CNF fibrils	1108:1118	CNF fibrils of ∼6 nm diameter	1108:1136	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	7	29	theme	∼6	1123:1124	arg1	nm					1126:1127	nm	1126:1127	nm	1126:1127	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	6	30	theme	CNF	1053:1055	arg1	network					1057:1063	a CNF network	1051:1063	a CNF network (native and modified)	1051:1085	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	1	31	theme	polymer	192:198	arg1	nanocomposites					200:213	polymer nanocomposites	192:213	polymer nanocomposites	192:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	0	32	theme	Nanostructural	0:13	arg1	Effects					15:21	Nanostructural Effects	0:21	Nanostructural Effects in High Cellulose Content	0:47	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	7	33	theme	CNF	1108:1110	arg1	fibrils					1112:1118	CNF fibrils	1108:1118	CNF fibrils of ∼6 nm diameter	1108:1136	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	2	34	theme	interface	333:341	arg1	manipulation					313:324	manipulation	313:324	manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA)	313:414	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	3	35	from	composition	576:586	arg1	minimum					545:551	a minimum	543:551	a minimum of changes in material composition	543:586	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	2	36	theme	methyl	388:393	arg1	poly					383:386	poly	383:386	poly(methyl methacrylate) (PMMA)	383:414	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	2	36	theme	methyl	388:393	arg1	methacrylate					395:406	methyl methacrylate	388:406	methyl methacrylate	388:406	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	0	37	theme	High	26:29	arg1	Content					41:47	High Cellulose Content	26:47	High Cellulose Content	26:47	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	4	38	theme	epoxide-hydroxyl	718:733	arg1	reaction					735:742	an epoxide-hydroxyl reaction	715:742	an epoxide-hydroxyl reaction	715:742	The hydroxyl-rich surface of CNF fibrils is exploited to modify the CNF surface via an epoxide-hydroxyl reaction.
30047261	10	39	theme	compositions	1606:1617	arg1	functionalities					1651:1665	functionalities	1651:1665	functionalities	1651:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	39	theme	compositions	1606:1617	arg1	architectures					1620:1632	architectures	1620:1632	architectures	1620:1632	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	39	theme	compositions	1606:1617	arg1	nanocomposites					1582:1595	cellulose nanocomposites	1572:1595	cellulose nanocomposites of other compositions, architectures, properties, and functionalities	1572:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	39	theme	compositions	1606:1617	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	39	theme	compositions	1606:1617	arg1	properties					1635:1644	properties	1635:1644	properties	1635:1644	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	5	40	theme	CNF	797:799	arg1	content					801:807	high CNF content	792:807	high CNF content (∼38 wt %)	792:818	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	5	40	theme	CNF	797:799	arg1	%					817:817	∼38 wt %	810:817	∼38 wt %	810:817	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	2	41	theme	optical	423:429	arg1	properties					456:465	the optical, thermal, and mechanical properties	419:465	the optical, thermal, and mechanical properties of the corresponding nanocomposites	419:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	6	42	theme	CNF	993:995	arg1	surface					997:1003	the modified CNF surface	980:1003	the modified CNF surface	980:1003	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	3	43	theme	CNF/PMMA	508:515	arg1	interface					517:525	The CNF/PMMA interface	504:525	The CNF/PMMA interface	504:525	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	5	44	theme	wt	814:815	arg1	content					801:807	high CNF content	792:807	high CNF content (∼38 wt %)	792:818	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	5	44	theme	wt	814:815	arg1	%					817:817	∼38 wt %	810:817	∼38 wt %	810:817	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	10	45	theme	properties	1635:1644	arg1	functionalities					1651:1665	functionalities	1651:1665	functionalities	1651:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	45	theme	properties	1635:1644	arg1	architectures					1620:1632	architectures	1620:1632	architectures	1620:1632	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	45	theme	properties	1635:1644	arg1	nanocomposites					1582:1595	cellulose nanocomposites	1572:1595	cellulose nanocomposites of other compositions, architectures, properties, and functionalities	1572:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	45	theme	properties	1635:1644	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	45	theme	properties	1635:1644	arg1	properties					1635:1644	properties	1635:1644	properties	1635:1644	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	8	46	theme	humidity	1284:1291	arg1	conditions					1293:1302	different relative humidity conditions	1265:1302	different relative humidity conditions	1265:1302	Moisture uptake and mechanical properties are measured at different relative humidity conditions.
30047261	7	47	theme	nm	1126:1127	arg1	diameter					1129:1136	∼6 nm diameter	1123:1136	∼6 nm diameter	1123:1136	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	8	48	theme	Moisture	1207:1214	arg1	uptake					1216:1221	Moisture uptake	1207:1221	Moisture uptake	1207:1221	Moisture uptake and mechanical properties are measured at different relative humidity conditions.
30047261	1	49	theme	nanocomposites	200:213	arg1	design					182:187	materials design	172:187	materials design of polymer nanocomposites	172:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	6	50	theme	solvent-assisted	1011:1026	arg1	diffusion					1028:1036	solvent-assisted diffusion	1011:1036	solvent-assisted diffusion of PMMA into a CNF network (native and modified)	1011:1085	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	0	51	theme	Cellulose	31:39	arg1	Content					41:47	High Cellulose Content	26:47	High Cellulose Content	26:47	Nanostructural Effects in High Cellulose Content Thermoplastic Nanocomposites with a Covalently Grafted Cellulose-Poly(methyl methacrylate) Interface.
30047261	9	52	theme	PMMA	1329:1332	arg1	molecules					1334:1342	PMMA molecules	1329:1342	PMMA molecules grafted to cellulose	1329:1363	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	5	53	theme	high	792:795	arg1	content					801:807	high CNF content	792:807	high CNF content (∼38 wt %)	792:818	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	5	53	theme	high	792:795	arg1	%					817:817	∼38 wt %	810:817	∼38 wt %	810:817	CNF/PMMA nanocomposites are then prepared with high CNF content (∼38 wt %) using an approach where a porous CNF mat is impregnated with monomer or polymer.
30047261	8	54	theme	mechanical	1227:1236	arg1	properties					1238:1247	mechanical properties	1227:1247	mechanical properties	1227:1247	Moisture uptake and mechanical properties are measured at different relative humidity conditions.
30047261	3	55	theme	interface	596:604	arg1	effects					606:612	interface effects	596:612	interface effects	596:612	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	4	56	theme	hydroxyl-rich	635:647	arg1	surface					649:655	The hydroxyl-rich surface	631:655	The hydroxyl-rich surface of CNF fibrils	631:670	The hydroxyl-rich surface of CNF fibrils is exploited to modify the CNF surface via an epoxide-hydroxyl reaction.
30047261	2	57	theme	mechanical	445:454	arg1	properties					456:465	the optical, thermal, and mechanical properties	419:465	the optical, thermal, and mechanical properties of the corresponding nanocomposites	419:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	7	58	theme	polymer	1170:1176	arg1	matrix					1178:1183	polymer matrix	1170:1183	polymer matrix	1170:1183	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	8	59	theme	different	1265:1273	arg1	conditions					1293:1302	different relative humidity conditions	1265:1302	different relative humidity conditions	1265:1302	Moisture uptake and mechanical properties are measured at different relative humidity conditions.
30047261	6	60	theme	nanocomposite	905:917	arg1	interface					919:927	The nanocomposite interface	901:927	The nanocomposite interface	901:927	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	10	61	theme	preparation	1515:1525	arg1	versatile					1542:1550	versatile	1542:1550	versatile	1542:1550	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	61	theme	preparation	1515:1525	arg1	strategies					1527:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	62	theme	architectures	1620:1632	arg1	functionalities					1651:1665	functionalities	1651:1665	functionalities	1651:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	62	theme	architectures	1620:1632	arg1	architectures					1620:1632	architectures	1620:1632	architectures	1620:1632	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	62	theme	architectures	1620:1632	arg1	nanocomposites					1582:1595	cellulose nanocomposites	1572:1595	cellulose nanocomposites of other compositions, architectures, properties, and functionalities	1572:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	62	theme	architectures	1620:1632	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	62	theme	architectures	1620:1632	arg1	properties					1635:1644	properties	1635:1644	properties	1635:1644	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	4	63	theme	CNF	699:701	arg1	surface					703:709	the CNF surface	695:709	the CNF surface	695:709	The hydroxyl-rich surface of CNF fibrils is exploited to modify the CNF surface via an epoxide-hydroxyl reaction.
30047261	2	64	from	effect	303:308	arg1	properties					456:465	the optical, thermal, and mechanical properties	419:465	the optical, thermal, and mechanical properties of the corresponding nanocomposites	419:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	7	65	theme	fibrils	1112:1118	arg1	content					1097:1103	The high content	1088:1103	The high content of CNF fibrils of ∼6 nm diameter	1088:1136	The high content of CNF fibrils of ∼6 nm diameter leads to a strong interface and polymer matrix distribution effects.
30047261	4	66	theme	fibrils	664:670	arg1	surface					649:655	The hydroxyl-rich surface	631:655	The hydroxyl-rich surface of CNF fibrils	631:670	The hydroxyl-rich surface of CNF fibrils is exploited to modify the CNF surface via an epoxide-hydroxyl reaction.
30047261	6	67	dep	network	1057:1063	arg1	native					1066:1071	native	1066:1071	native	1066:1071	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	6	67	dep	network	1057:1063	arg1	modified					1077:1084	modified	1077:1084	modified	1077:1084	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	3	68	from	changes	556:562	arg1	composition					576:586	material composition	567:586	material composition	567:586	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	9	69	with	nanocomposites	1309:1322	arg1	molecules					1334:1342	PMMA molecules	1329:1342	PMMA molecules grafted to cellulose	1329:1363	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	10	70	used	used	1563:1566	arg2	strategies					1527:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies	1486:1536	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	70	used	used	1563:1566	arg2	versatile					1542:1550	versatile	1542:1550	versatile	1542:1550	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	6	71	theme	PMMA	1041:1044	arg1	diffusion					1028:1036	solvent-assisted diffusion	1011:1036	solvent-assisted diffusion of PMMA into a CNF network (native and modified)	1011:1085	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
30047261	2	72	theme	cellulose	351:359	arg1	CNF					374:376	CNF	374:376	CNF	374:376	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	2	72	theme	cellulose	351:359	arg1	nanofibrils					361:371	cellulose nanofibrils	351:371	cellulose nanofibrils (CNF)	351:377	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	1	73	theme	nanoparticle/polymer	236:255	arg1	interface					257:265	the nanoparticle/polymer interface	232:265	the nanoparticle/polymer interface	232:265	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	9	74	theme	control	1469:1475	arg1	samples					1477:1483	the control samples	1465:1483	the control samples	1465:1483	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	3	75	from	minimum	545:551	arg1	composition					576:586	material composition	567:586	material composition	567:586	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	2	76	theme	present	272:278	arg1	study					280:284	The present study	268:284	The present study	268:284	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	1	77	theme	critical	153:160	arg1	aspect					162:167	A critical aspect	151:167	A critical aspect in materials design of polymer nanocomposites	151:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	1	77	theme	critical	153:160	arg1	nature					222:227	the nature	218:227	the nature of the nanoparticle/polymer interface	218:265	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	10	78	theme	functionalities	1651:1665	arg1	functionalities					1651:1665	functionalities	1651:1665	functionalities	1651:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	78	theme	functionalities	1651:1665	arg1	architectures					1620:1632	architectures	1620:1632	architectures	1620:1632	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	78	theme	functionalities	1651:1665	arg1	nanocomposites					1582:1595	cellulose nanocomposites	1572:1595	cellulose nanocomposites of other compositions, architectures, properties, and functionalities	1572:1665	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	78	theme	functionalities	1651:1665	arg1	compositions					1606:1617	other compositions	1600:1617	other compositions	1600:1617	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	10	78	theme	functionalities	1651:1665	arg1	properties					1635:1644	properties	1635:1644	properties	1635:1644	The present modification and preparation strategies are versatile and may be used for cellulose nanocomposites of other compositions, architectures, properties, and functionalities.
30047261	1	79	theme	interface	257:265	arg1	aspect					162:167	A critical aspect	151:167	A critical aspect in materials design of polymer nanocomposites	151:213	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	1	79	theme	interface	257:265	arg1	nature					222:227	the nature	218:227	the nature of the nanoparticle/polymer interface	218:265	A critical aspect in materials design of polymer nanocomposites is the nature of the nanoparticle/polymer interface.
30047261	2	80	theme	nanocomposites	488:501	arg1	properties					456:465	the optical, thermal, and mechanical properties	419:465	the optical, thermal, and mechanical properties of the corresponding nanocomposites	419:501	The present study investigates the effect of manipulation of the interface between cellulose nanofibrils (CNF) and poly(methyl methacrylate) (PMMA) on the optical, thermal, and mechanical properties of the corresponding nanocomposites.
30047261	9	81	theme	higher	1380:1385	arg1	transparency					1395:1406	much higher optical transparency	1375:1406	much higher optical transparency	1375:1406	The nanocomposites with PMMA molecules grafted to cellulose exhibited much higher optical transparency, thermal stability, and hygro-mechanical properties than the control samples.
30047261	3	82	theme	material	567:574	arg1	composition					576:586	material composition	567:586	material composition	567:586	The CNF/PMMA interface is altered with a minimum of changes in material composition so that interface effects can be analyzed.
30047261	6	83	theme	modified	984:991	arg1	surface					997:1003	the modified CNF surface	980:1003	the modified CNF surface	980:1003	The nanocomposite interface is controlled by either providing PMMA grafts from the modified CNF surface or by solvent-assisted diffusion of PMMA into a CNF network (native and modified).
31546404	4	0	theme	embryonic	904:912	arg1	chick					914:918	embryonic chick	904:918	embryonic chick	904:918	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	2	1	from	present	436:442	arg1	composite					465:473	the polymer-based composite	447:473	the polymer-based composite	447:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	7	2	theme	nanoHA	1298:1303	arg1	content					1309:1315	nanoHA 30% content	1298:1315	nanoHA 30% content	1298:1315	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	8	3	theme	nanoHA	1710:1715	arg1	content					1717:1723	higher nanoHA content	1703:1723	higher nanoHA content	1703:1723	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	3	4	from	influence	513:521	arg1	hydrogels					586:594	alginate-based hydrogels	571:594	alginate-based hydrogels	571:594	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	4	5	theme	segmental	920:928	arg1	defects					935:941	segmental bone defects	920:941	segmental bone defects	920:941	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	8	6	with	compositions	1685:1696	arg1	content					1717:1723	higher nanoHA content	1703:1723	higher nanoHA content	1703:1723	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	7	7	theme	osteogenic	1477:1486	arg1	response					1493:1500	the osteogenic cell response	1473:1500	the osteogenic cell response	1473:1500	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	9	8	theme	bone	2008:2011	arg1	regeneration					2020:2031	bone tissue regeneration	2008:2031	bone tissue regeneration	2008:2031	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	4	9	dep	assessment	826:835	arg1	defects					935:941	segmental bone defects	920:941	segmental bone defects	920:941	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	9	10	theme	nanohydroxyapatite	1809:1826	arg1	concentration					1828:1840	nanohydroxyapatite concentration	1809:1840	nanohydroxyapatite concentration	1809:1840	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	3	11	theme	alginate-based	571:584	arg1	hydrogels					586:594	alginate-based hydrogels	571:594	alginate-based hydrogels	571:594	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	1	12	theme	injured	218:224	arg1	bone					238:241	injured or diseased bone	218:241	injured or diseased bone	218:241	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	7	13	theme	30	1305:1306	arg1	%					1307:1307	%	1307:1307	%	1307:1307	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	1	14	theme	diseased	229:236	arg1	bone					238:241	injured or diseased bone	218:241	injured or diseased bone	218:241	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	1	15	theme	potential	156:164	arg1	biomaterials					166:177	potential biomaterials	156:177	potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability	156:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	9	16	theme	study	1789:1793	arg1	outcomes					1772:1779	The outcomes	1768:1779	The outcomes of this study	1768:1793	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	8	17	theme	Ex	1503:1504	arg1	data					1511:1514	Ex vivo data	1503:1514	Ex vivo data	1503:1514	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	6	18	theme	apatite	1157:1163	arg1	formation					1165:1173	apatite formation	1157:1173	apatite formation	1157:1173	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	5	19	theme	morphological	953:965	arg1	characteristics					967:981	The main morphological characteristics	944:981	The main morphological characteristics of the alginate network	944:1005	The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite.
31546404	7	20	theme	higher	1428:1433	arg1	%					1460:1460	50 and 70%	1451:1460	50 and 70%	1451:1460	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	7	20	theme	higher	1428:1433	arg1	concentrations					1435:1448	higher concentrations	1428:1448	higher concentrations (50 and 70%)	1428:1461	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	4	21	theme	biological	773:782	arg1	response					784:791	biological response	773:791	biological response	773:791	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	3	22	dep	70 wt	560:564	arg1	to					557:558	to	557:558	to	557:558	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	6	23	theme	physic-chemical	1073:1087	arg1	features					1089:1096	physic-chemical features	1073:1096	physic-chemical features	1073:1096	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	7	24	with	hydrogels	1283:1291	arg1	content					1309:1315	nanoHA 30% content	1298:1315	nanoHA 30% content	1298:1315	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	4	25	theme	composite	695:703	arg1	system					705:710	The composite system	691:710	The composite system	691:710	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	0	26	theme	enhanced	76:83	arg1	regeneration					90:101	enhanced bone regeneration	76:101	enhanced bone regeneration	76:101	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.
31546404	4	27	with	evaluation	890:899	arg1	cells					861:865	human osteoblastic cells	842:865	human osteoblastic cells	842:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	8	28	dep	in	1531:1532	arg1	vitro					1534:1538	vitro	1534:1538	vitro	1534:1538	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	9	29	theme	physic-chemical	1864:1878	arg1	properties					1880:1889	physic-chemical properties	1864:1889	physic-chemical properties	1864:1889	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	2	30	from	composite	465:473	arg1	present					436:442	present	436:442	present	436:442	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	7	31	dep	in	1228:1229	arg1	vitro					1231:1235	vitro	1231:1235	vitro	1231:1235	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	4	32	from	evaluation	890:899	arg1	chick					914:918	embryonic chick	904:918	embryonic chick	904:918	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	8	33	theme	collagenous	1572:1582	arg1	deposition					1584:1593	an enhanced collagenous deposition	1560:1593	an enhanced collagenous deposition	1560:1593	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	6	34	theme	water-swelling	1104:1117	arg1	rate					1119:1122	water-swelling rate	1104:1122	water-swelling rate	1104:1122	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	4	35	theme	in	799:800	arg1	assessment					826:835	in vitro cytocompatibility assessment	799:835	in vitro cytocompatibility assessment with human osteoblastic cells	799:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	4	36	theme	properties	758:767	arg1	terms					733:737	terms	733:737	terms of physic-chemical properties and biological response	733:791	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	0	37	theme	Alginate-nanohydroxyapatite	0:26	arg1	system					37:42	Alginate-nanohydroxyapatite hydrogel system	0:42	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.	0:102	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.
31546404	1	38	theme	biocompatibility	290:305	arg1	terms					281:285	terms	281:285	terms of biocompatibility, bioactivity, injectability, and biodegradability	281:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	5	39	theme	alginate	990:997	arg1	network					999:1005	the alginate network	986:1005	the alginate network	986:1005	The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite.
31546404	7	40	theme	factors	1402:1408	arg1	expression					1363:1372	expression	1363:1372	expression of osteogenic transcription factors	1363:1408	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	7	40	theme	factors	1402:1408	arg1	proliferation					1345:1357	osteoblastic cells proliferation	1326:1357	osteoblastic cells proliferation	1326:1357	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	2	41	theme	polymer-based	451:463	arg1	composite					465:473	the polymer-based composite	447:473	the polymer-based composite	447:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	8	42	theme	bone	1607:1610	arg1	formation					1612:1620	trabecular bone formation	1596:1620	trabecular bone formation	1596:1620	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	1	43	dep	fill	182:185	arg1	bone					238:241	injured or diseased bone	218:241	injured or diseased bone	218:241	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	4	44	dep	ex	871:872	arg1	vivo					874:877	vivo	874:877	vivo	874:877	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	1	45	theme	bioactivity	308:318	arg1	terms					281:285	terms	281:285	terms of biocompatibility, bioactivity, injectability, and biodegradability	281:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	2	46	attach	present	436:442	arg1	composite					465:473	the polymer-based composite	447:473	the polymer-based composite	447:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	2	46	attach	present	436:442	arg2	component					426:434	ceramic component	418:434	ceramic component	418:434	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	2	46	attach	present	436:442	arg2	amount					408:413	the amount	404:413	the amount of ceramic component present in the polymer-based composite	404:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	7	47	theme	osteogenic	1377:1386	arg1	factors					1402:1408	osteogenic transcription factors	1377:1408	osteogenic transcription factors	1377:1408	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	9	48	theme	system	1932:1937	arg1	properties					1880:1889	physic-chemical properties	1864:1889	physic-chemical properties	1864:1889	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	9	48	theme	system	1932:1937	arg1	response					1906:1913	biological response	1895:1913	biological response	1895:1913	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	4	49	theme	osteoblastic	848:859	arg1	cells					861:865	human osteoblastic cells	842:865	human osteoblastic cells	842:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	2	50	theme	component	426:434	arg1	amount					408:413	the amount	404:413	the amount of ceramic component present in the polymer-based composite	404:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	2	50	theme	component	426:434	arg1	component					426:434	ceramic component	418:434	ceramic component	418:434	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	1	51	theme	injectability	321:333	arg1	terms					281:285	terms	281:285	terms of biocompatibility, bioactivity, injectability, and biodegradability	281:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	8	52	theme	Alg-nanoHA30	1653:1664	arg1	composition					1666:1676	Alg-nanoHA30 composition	1653:1676	Alg-nanoHA30 composition	1653:1676	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	4	53	theme	cytocompatibility	808:824	arg1	assessment					826:835	in vitro cytocompatibility assessment	799:835	in vitro cytocompatibility assessment with human osteoblastic cells	799:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	3	54	theme	present	494:500	arg1	study					502:506	the present study	490:506	the present study	490:506	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	1	55	theme	due	244:246	arg1	fill					182:185	fill	182:185	fill	182:185	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	0	56	dep	system	37:42	arg1	Optimizing					45:54	Optimizing	45:54	Optimizing the formulation for enhanced bone regeneration	45:101	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.
31546404	7	57	theme	osteoblastic	1326:1337	arg1	cells					1339:1343	osteoblastic cells	1326:1343	osteoblastic cells proliferation	1326:1357	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	9	58	theme	components	1967:1976	arg1	ratio					1978:1982	the components ratio	1963:1982	the components ratio	1963:1982	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	3	59	theme	tissue	670:675	arg1	regeneration					677:688	bone tissue regeneration	665:688	bone tissue regeneration	665:688	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	3	60	theme	nanohydroxyapatite	526:543	arg1	%					565:565	30 to 70 wt%	554:565	30 to 70 wt%	554:565	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	3	60	theme	nanohydroxyapatite	526:543	arg1	content					545:551	nanohydroxyapatite content	526:551	nanohydroxyapatite content (30 to 70 wt%)	526:566	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	8	61	theme	bone	1746:1749	arg1	response					1758:1765	a diminished bone tissue response	1733:1765	a diminished bone tissue response	1733:1765	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	7	62	located	observed	1269:1276	arg1	assays					1255:1260	in vitro cytocompatibility assays	1228:1260	in vitro cytocompatibility assays	1228:1260	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	7	62	located	observed	1269:1276	arg2	it					1262:1263	it	1262:1263	it	1262:1263	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	6	63	theme	pH	1146:1147	arg1	values					1149:1154	extreme pH values	1138:1154	extreme pH values	1138:1154	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	8	64	theme	higher	1703:1708	arg1	content					1717:1723	higher nanoHA content	1703:1723	higher nanoHA content	1703:1723	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	7	65	theme	cells	1339:1343	arg1	proliferation					1345:1357	osteoblastic cells proliferation	1326:1357	osteoblastic cells proliferation	1326:1357	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	4	66	theme	ex	871:872	arg1	evaluation					890:899	ex vivo functional evaluation	871:899	ex vivo functional evaluation in embryonic chick	871:918	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	7	67	theme	cell	1488:1491	arg1	response					1493:1500	the osteogenic cell response	1473:1500	the osteogenic cell response	1473:1500	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	9	68	theme	tissue	2013:2018	arg1	regeneration					2020:2031	bone tissue regeneration	2008:2031	bone tissue regeneration	2008:2031	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	4	69	theme	functional	879:888	arg1	evaluation					890:899	ex vivo functional evaluation	871:899	ex vivo functional evaluation in embryonic chick	871:918	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	1	70	from	features	269:276	arg1	terms					281:285	terms	281:285	terms of biocompatibility, bioactivity, injectability, and biodegradability	281:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	8	71	dep	Ex	1503:1504	arg1	vivo					1506:1509	vivo	1506:1509	vivo	1506:1509	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	5	72	theme	main	948:951	arg1	characteristics					967:981	The main morphological characteristics	944:981	The main morphological characteristics of the alginate network	944:1005	The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite.
31546404	4	73	theme	bone	930:933	arg1	defects					935:941	segmental bone defects	920:941	segmental bone defects	920:941	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	8	74	theme	in	1531:1532	arg1	findings					1540:1547	the in vitro findings	1527:1547	the in vitro findings	1527:1547	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	0	75	theme	bone	85:88	arg1	regeneration					90:101	enhanced bone regeneration	76:101	enhanced bone regeneration	76:101	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.
31546404	4	76	from	assessment	826:835	arg1	chick					914:918	embryonic chick	904:918	embryonic chick	904:918	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	4	77	dep	in	799:800	arg1	vitro					802:806	vitro	802:806	vitro	802:806	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	9	78	theme	major	1850:1854	arg1	role					1856:1859	a major role	1848:1859	a major role	1848:1859	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	8	79	theme	enhanced	1563:1570	arg1	deposition					1584:1593	an enhanced collagenous deposition	1560:1593	an enhanced collagenous deposition	1560:1593	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	4	80	with	assessment	826:835	arg1	cells					861:865	human osteoblastic cells	842:865	human osteoblastic cells	842:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	4	81	theme	response	784:791	arg1	terms					733:737	terms	733:737	terms of physic-chemical properties and biological response	733:791	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	7	82	theme	cytocompatibility	1237:1253	arg1	assays					1255:1260	in vitro cytocompatibility assays	1228:1260	in vitro cytocompatibility assays	1228:1260	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	6	83	theme	extreme	1138:1144	arg1	values					1149:1154	extreme pH values	1138:1154	extreme pH values	1138:1154	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	9	84	theme	biological	1895:1904	arg1	response					1906:1913	biological response	1895:1913	biological response	1895:1913	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	8	85	theme	trabecular	1596:1605	arg1	formation					1612:1620	trabecular bone formation	1596:1620	trabecular bone formation	1596:1620	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	0	86	theme	hydrogel	28:35	arg1	system					37:42	Alginate-nanohydroxyapatite hydrogel system	0:42	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.	0:102	Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration.
31546404	1	87	theme	Ceramic/polymer-based	104:124	arg1	biocomposites					126:138	Ceramic/polymer-based biocomposites	104:138	Ceramic/polymer-based biocomposites	104:138	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	7	88	theme	transcription	1388:1400	arg1	factors					1402:1408	osteogenic transcription factors	1377:1408	osteogenic transcription factors	1377:1408	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	4	89	theme	physic-chemical	742:756	arg1	properties					758:767	physic-chemical properties	742:767	physic-chemical properties	742:767	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	5	90	theme	network	999:1005	arg1	characteristics					967:981	The main morphological characteristics	944:981	The main morphological characteristics of the alginate network	944:1005	The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite.
31546404	9	91	theme	composite	1922:1930	arg1	system					1932:1937	the composite system	1918:1937	the composite system	1918:1937	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	8	92	theme	matrix	1626:1631	arg1	mineralization					1633:1646	matrix mineralization	1626:1646	matrix mineralization	1626:1646	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	2	93	theme	present	436:442	arg1	amount					408:413	the amount	404:413	the amount of ceramic component present in the polymer-based composite	404:473	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	2	93	theme	present	436:442	arg1	component					426:434	ceramic component	418:434	ceramic component	418:434	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	3	94	theme	best	633:636	arg1	formulation					638:648	the best formulation	629:648	the best formulation for maximizing bone tissue regeneration	629:688	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	4	95	theme	human	842:846	arg1	cells					861:865	human osteoblastic cells	842:865	human osteoblastic cells	842:865	The composite system was characterized in terms of physic-chemical properties and biological response, with in vitro cytocompatibility assessment with human osteoblastic cells and ex vivo functional evaluation in embryonic chick segmental bone defects.
31546404	2	96	theme	ceramic	418:424	arg1	component					426:434	ceramic component	418:434	ceramic component	418:434	However, these properties can be dependent on the amount of ceramic component present in the polymer-based composite.
31546404	6	97	theme	Ca2+	1180:1183	arg1	release					1185:1191	Ca2+ release	1180:1191	Ca2+ release	1180:1191	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	8	98	theme	diminished	1735:1744	arg1	response					1758:1765	a diminished bone tissue response	1733:1765	a diminished bone tissue response	1733:1765	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	1	99	theme	biodegradability	340:355	arg1	terms					281:285	terms	281:285	terms of biocompatibility, bioactivity, injectability, and biodegradability	281:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31546404	3	100	theme	bone	665:668	arg1	regeneration					677:688	bone tissue regeneration	665:688	bone tissue regeneration	665:688	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	6	101	from	values	1149:1154	arg1	stability					1125:1133	stability	1125:1133	stability at extreme pH values	1125:1154	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	6	101	from	values	1149:1154	arg1	release					1185:1191	Ca2+ release	1180:1191	Ca2+ release	1180:1191	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	6	101	from	values	1149:1154	arg1	rate					1119:1122	water-swelling rate	1104:1122	water-swelling rate	1104:1122	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	6	101	from	values	1149:1154	arg1	formation					1165:1173	apatite formation	1157:1173	apatite formation	1157:1173	However, physic-chemical features, like water-swelling rate, stability at extreme pH values, apatite formation, and Ca2+ release were nanoHA dose-dependent.
31546404	3	102	theme	content	545:551	arg1	influence					513:521	the influence	509:521	the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels	509:594	Therefore, in the present study, the influence of nanohydroxyapatite content (30 to 70 wt%) on alginate-based hydrogels was studied in order to evaluate the best formulation for maximizing bone tissue regeneration.
31546404	8	103	theme	tissue	1751:1756	arg1	response					1758:1765	a diminished bone tissue response	1733:1765	a diminished bone tissue response	1733:1765	Ex vivo data underlined the in vitro findings, revealing an enhanced collagenous deposition, trabecular bone formation and matrix mineralization with Alg-nanoHA30 composition, while compositions with higher nanoHA content induced a diminished bone tissue response.
31546404	5	104	theme	nanohydroxyapatite	1044:1061	arg1	addition					1032:1039	the addition	1028:1039	the addition of nanohydroxyapatite	1028:1061	The main morphological characteristics of the alginate network were not affected by the addition of nanohydroxyapatite.
31546404	7	105	theme	%	1307:1307	arg1	content					1309:1315	nanoHA 30% content	1298:1315	nanoHA 30% content	1298:1315	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	9	106	theme	ratio	1978:1982	arg1	met					1992:1994	met	1992:1994	met	1992:1994	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	9	106	theme	ratio	1978:1982	arg1	optimization					1947:1958	the optimization	1943:1958	the optimization of the components ratio	1943:1982	The outcomes of this study indicate that nanohydroxyapatite concentration plays a major role in physic-chemical properties and biological response of the composite system and the optimization of the components ratio must be met to maximize bone tissue regeneration.
31546404	7	107	theme	in	1228:1229	arg1	assays					1255:1260	in vitro cytocompatibility assays	1228:1260	in vitro cytocompatibility assays	1228:1260	Within in vitro cytocompatibility assays it was observed that hydrogels with nanoHA 30% content enhanced osteoblastic cells proliferation and expression of osteogenic transcription factors, while those with higher concentrations (50 and 70%) decreased the osteogenic cell response.
31546404	1	108	theme	outstanding	257:267	arg1	features					269:276	their outstanding features	251:276	their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability	251:355	Ceramic/polymer-based biocomposites have emerged as potential biomaterials to fill, replace, repair or regenerate injured or diseased bone, due to their outstanding features in terms of biocompatibility, bioactivity, injectability, and biodegradability.
31525592	6	0	theme	2.8 μkat/kg	993:1003	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	7	1	theme	structural	1122:1131	arg1	identity					1133:1140	the structural identity	1118:1140	the structural identity	1118:1140	Furthermore, the major reaction product was isolated and subsequently, the structural identity was elucidated and verified via extensive NMR analysis.
31525592	0	2	from	generation	10:19	arg1	sweet					37:41	sweet	37:41	sweet	37:41	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	0	2	from	generation	10:19	arg1	whey					52:55	acid whey	47:55	acid whey	47:55	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	1	3	from	rising	149:154	arg1	interest					159:166	interest	159:166	interest in commercial scale productions due to increasing health awareness of consumers	159:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	2	4	theme	enzymatic	336:344	arg1	generation					346:355	the enzymatic generation	332:355	the enzymatic generation of prebiotic lactulose	332:378	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	4	5	theme	concentration	568:580	arg1	activity					603:610	enzymatic activity	593:610	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	4	5	theme	concentration	568:580	arg1	influence					534:542	The influence	530:542	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	0	6	theme	composition	79:89	arg1	Optimization					58:69	Optimization	58:69	Optimization of feed composition	58:89	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	0	6	theme	composition	79:89	arg1	elucidation					106:116	structural elucidation	95:116	structural elucidation of 1-lactulose	95:131	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	3	7	from	variation	404:412	arg1	composition					417:427	composition	417:427	composition	417:427	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	3	8	contain	has	392:394	arg2	variation					404:412	a broad variation	396:412	a broad variation in composition	396:427	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	3	8	contain	has	392:394	arg1	whey					387:390	whey	387:390	whey	387:390	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	2	9	theme	acid	287:290	arg1	whey					292:295	acid whey	287:295	acid whey	287:295	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	0	10	theme	feed	74:77	arg1	composition					79:89	feed composition	74:89	feed composition	74:89	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	6	11	theme	theoretical	837:847	arg1	lactulose					867:875	lactulose	867:875	lactulose	867:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	11	theme	theoretical	837:847	arg1	amount					857:862	a theoretical maximal amount	835:862	a theoretical maximal amount of lactulose	835:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	0	12	theme	structural	95:104	arg1	elucidation					106:116	structural elucidation	95:116	structural elucidation of 1-lactulose	95:131	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	5	13	contain	containing	758:767	arg2	terms					790:794	pairwise interaction terms	769:794	pairwise interaction terms	769:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	5	13	contain	containing	758:767	arg1	model					752:756	a quadratic model	740:756	a quadratic model containing pairwise interaction terms	740:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	3	14	theme	feed	451:454	arg1	composition					456:466	the feed composition	447:466	the feed composition	447:466	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	6	15	with	1.28	895:898	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	5	16	theme	pairwise	769:776	arg1	terms					790:794	pairwise interaction terms	769:794	pairwise interaction terms	769:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	7	17	theme	NMR	1184:1186	arg1	analysis					1188:1195	extensive NMR analysis	1174:1195	extensive NMR analysis	1174:1195	Furthermore, the major reaction product was isolated and subsequently, the structural identity was elucidated and verified via extensive NMR analysis.
31525592	2	18	theme	suitable	307:314	arg1	medium					321:326	a suitable feed medium	305:326	a suitable feed medium for the enzymatic generation of prebiotic lactulose	305:378	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	3	19	theme	lactulose	502:510	arg1	concentration					475:487	the concentration	471:487	the concentration of generated lactulose	471:510	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	1	20	theme	commercial	171:180	arg1	productions					188:198	commercial scale productions	171:198	commercial scale productions due to increasing health awareness of consumers	171:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	0	21	theme	Enzymatic	0:8	arg1	generation					10:19	Enzymatic generation	0:19	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.	0:132	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	4	22	theme	lactose	547:553	arg1	activity					603:610	enzymatic activity	593:610	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	4	22	theme	lactose	547:553	arg1	influence					534:542	The influence	530:542	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	6	23	theme	feed	809:812	arg1	composition					814:824	The optimal feed composition	797:824	The optimal feed composition yielding a theoretical maximal amount of lactulose	797:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	23	theme	feed	809:812	arg1	lactose					948:954	0.17 or 0.19 mol/kg lactose	928:954	0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	928:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	23	theme	feed	809:812	arg1	fructose					915:922	0.74 mol/kg fructose	903:922	0.74 mol/kg fructose	903:922	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	23	theme	feed	809:812	arg1	1.28					895:898	1.28	895:898	1.28	895:898	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	2	24	theme	lactulose	370:378	arg1	generation					346:355	the enzymatic generation	332:355	the enzymatic generation of prebiotic lactulose	332:378	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	0	25	theme	lactulose	24:32	arg1	generation					10:19	Enzymatic generation	0:19	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.	0:132	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	5	26	theme	surface	718:724	arg1	analysis					726:733	response surface analysis	709:733	response surface analysis with a quadratic model containing pairwise interaction terms	709:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	6	27	theme	optimal	801:807	arg1	composition					814:824	The optimal feed composition	797:824	The optimal feed composition yielding a theoretical maximal amount of lactulose	797:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	27	theme	optimal	801:807	arg1	lactose					948:954	0.17 or 0.19 mol/kg lactose	928:954	0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	928:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	27	theme	optimal	801:807	arg1	fructose					915:922	0.74 mol/kg fructose	903:922	0.74 mol/kg fructose	903:922	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	27	theme	optimal	801:807	arg1	1.28					895:898	1.28	895:898	1.28	895:898	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	4	28	theme	available	632:640	arg1	β-galactosidases					642:657	two commercially available β-galactosidases	615:657	two commercially available β-galactosidases	615:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	7	29	theme	extensive	1174:1182	arg1	analysis					1188:1195	extensive NMR analysis	1174:1195	extensive NMR analysis	1174:1195	Furthermore, the major reaction product was isolated and subsequently, the structural identity was elucidated and verified via extensive NMR analysis.
31525592	4	30	theme	fructose	559:566	arg1	concentration					568:580	fructose concentration	559:580	fructose concentration	559:580	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	6	31	theme	0.74 mol/kg	903:913	arg1	composition					814:824	The optimal feed composition	797:824	The optimal feed composition yielding a theoretical maximal amount of lactulose	797:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	31	theme	0.74 mol/kg	903:913	arg1	fructose					915:922	0.74 mol/kg fructose	903:922	0.74 mol/kg fructose	903:922	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	3	32	theme	generated	492:500	arg1	lactulose					502:510	generated lactulose	492:510	generated lactulose	492:510	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	1	33	theme	due	200:202	arg1	productions					188:198	commercial scale productions	171:198	commercial scale productions due to increasing health awareness of consumers	171:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	3	34	theme	broad	398:402	arg1	variation					404:412	a broad variation	396:412	a broad variation in composition	396:427	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	1	35	theme	scale	182:186	arg1	productions					188:198	commercial scale productions	171:198	commercial scale productions due to increasing health awareness of consumers	171:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	6	36	theme	2.0	986:988	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	0	37	theme	1-lactulose	121:131	arg1	Optimization					58:69	Optimization	58:69	Optimization of feed composition	58:89	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	0	37	theme	1-lactulose	121:131	arg1	elucidation					106:116	structural elucidation	95:116	structural elucidation of 1-lactulose	95:131	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	3	38	from	influence	434:442	arg1	concentration					475:487	the concentration	471:487	the concentration of generated lactulose	471:510	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	4	39	theme	β-galactosidases	642:657	arg1	activity					603:610	enzymatic activity	593:610	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	4	39	theme	β-galactosidases	642:657	arg1	influence					534:542	The influence	530:542	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	0	40	theme	acid	47:50	arg1	whey					52:55	acid whey	47:55	acid whey	47:55	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	5	41	theme	quadratic	742:750	arg1	model					752:756	a quadratic model	740:756	a quadratic model containing pairwise interaction terms	740:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	6	42	with	lactose	948:954	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	7	43	theme	major	1064:1068	arg1	product					1079:1085	the major reaction product	1060:1085	the major reaction product	1060:1085	Furthermore, the major reaction product was isolated and subsequently, the structural identity was elucidated and verified via extensive NMR analysis.
31525592	7	44	theme	reaction	1070:1077	arg1	product					1079:1085	the major reaction product	1060:1085	the major reaction product	1060:1085	Furthermore, the major reaction product was isolated and subsequently, the structural identity was elucidated and verified via extensive NMR analysis.
31525592	6	45	theme	enzymatic	964:972	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	46	theme	maximal	849:855	arg1	lactulose					867:875	lactulose	867:875	lactulose	867:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	46	theme	maximal	849:855	arg1	amount					857:862	a theoretical maximal amount	835:862	a theoretical maximal amount of lactulose	835:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	3	47	theme	composition	456:466	arg1	influence					434:442	the influence	430:442	the influence of the feed composition on the concentration of generated lactulose	430:510	Since whey has a broad variation in composition, the influence of the feed composition on the concentration of generated lactulose was investigated.
31525592	1	48	theme	health	218:223	arg1	awareness					225:233	health awareness	218:233	health awareness of consumers	218:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	5	49	theme	response	709:716	arg1	analysis					726:733	response surface analysis	709:733	response surface analysis with a quadratic model containing pairwise interaction terms	709:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	6	50	theme	lactulose	867:875	arg1	lactulose					867:875	lactulose	867:875	lactulose	867:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	6	50	theme	lactulose	867:875	arg1	amount					857:862	a theoretical maximal amount	835:862	a theoretical maximal amount of lactulose	835:875	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	2	51	theme	bio-economic	255:266	arg1	aspects					268:274	bio-economic aspects	255:274	bio-economic aspects	255:274	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	2	52	theme	feed	316:319	arg1	medium					321:326	a suitable feed medium	305:326	a suitable feed medium for the enzymatic generation of prebiotic lactulose	305:378	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	4	53	theme	enzymatic	593:601	arg1	activity					603:610	enzymatic activity	593:610	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases	530:657	The influence of lactose and fructose concentration as well as enzymatic activity of two commercially available β-galactosidases were investigated.
31525592	5	54	theme	interaction	778:788	arg1	terms					790:794	pairwise interaction terms	769:794	pairwise interaction terms	769:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	5	55	with	analysis	726:733	arg1	model					752:756	a quadratic model	740:756	a quadratic model containing pairwise interaction terms	740:794	The results were evaluated via response surface analysis with a quadratic model containing pairwise interaction terms.
31525592	0	56	dep	generation	10:19	arg1	Optimization					58:69	Optimization	58:69	Optimization of feed composition	58:89	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	0	56	dep	generation	10:19	arg1	elucidation					106:116	structural elucidation	95:116	structural elucidation of 1-lactulose	95:131	Enzymatic generation of lactulose in sweet and acid whey: Optimization of feed composition and structural elucidation of 1-lactulose.
31525592	2	57	theme	prebiotic	360:368	arg1	lactulose					370:378	prebiotic lactulose	360:378	prebiotic lactulose	360:378	Under bio-economic aspects, sweet and acid whey provide a suitable feed medium for the enzymatic generation of prebiotic lactulose.
31525592	6	58	theme	sweet	1026:1030	arg1	whey					1041:1044	sweet (pH 6.6) whey	1026:1044	sweet (pH 6.6) whey	1026:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	1	59	from	interest	159:166	arg1	rising					149:154	rising	149:154	rising	149:154	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	1	59	from	interest	159:166	arg1	productions					188:198	commercial scale productions	171:198	commercial scale productions due to increasing health awareness of consumers	171:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
31525592	6	60	with	fructose	915:922	arg1	activity					974:981	an enzymatic activity	961:981	an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey	961:1044	The optimal feed composition yielding a theoretical maximal amount of lactulose was determined as 1.28 or 0.74 mol/kg fructose and 0.17 or 0.19 mol/kg lactose with an enzymatic activity of 2.0 or 2.8 μkat/kg for acid (pH 4.4) or sweet (pH 6.6) whey.
31525592	1	61	theme	consumers	238:246	arg1	awareness					225:233	health awareness	218:233	health awareness of consumers	218:246	Prebiotics are rising in interest in commercial scale productions due to increasing health awareness of consumers.
30261126	4	0	theme	purification	951:962	arg1	process					924:930	the process	920:930	the process of cultivation and purification	920:962	The obtained results indicate that the composites components are well integrated after the process of cultivation and purification.
30261126	3	1	theme	N-acetyl-β-d-hexosaminidase	773:799	arg1	release					801:807	N-acetyl-β-d-hexosaminidase release	773:807	N-acetyl-β-d-hexosaminidase release	773:807	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	6	2	theme	immunogenic	1201:1211	arg1	composites					1185:1194	the composites	1181:1194	the composites less immunogenic	1181:1211	Moreover, application of bacterial cellulose makes the composites less immunogenic as compared to polypropylene itself.
30261126	7	3	from	potential	1291:1299	arg1	medicine					1319:1326	medicine	1319:1326	medicine	1319:1326	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	1	4	theme	reconstructive	272:285	arg1	surgery					287:293	reconstructive surgery	272:293	reconstructive surgery of soft and hard tissues	272:318	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	0	5	theme	biomedical	82:91	arg1	applications					93:104	biomedical applications	82:104	biomedical applications	82:104	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	7	6	theme	protective	1529:1538	arg1	barrier					1540:1546	a protective barrier	1527:1546	a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement	1527:1635	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	7	theme	applied	1350:1356	arg1	material					1365:1372	the applied porous material	1346:1372	the applied porous material	1346:1372	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	2	8	theme	stationary	578:587	arg1	culture					589:595	stationary culture	578:595	stationary culture	578:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	3	9	theme	mast	744:747	arg1	degranulation					754:766	mast cell degranulation	744:766	mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion	744:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	4	10	theme	obtained	837:844	arg1	results					846:852	The obtained results	833:852	The obtained results	833:852	The obtained results indicate that the composites components are well integrated after the process of cultivation and purification.
30261126	3	11	with	degranulation	754:766	arg1	adhesion					823:830	mast cell adhesion	813:830	mast cell adhesion	813:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	3	11	with	degranulation	754:766	arg1	release					801:807	N-acetyl-β-d-hexosaminidase release	773:807	N-acetyl-β-d-hexosaminidase release	773:807	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	3	12	theme	developed	602:610	arg1	biocomposites					612:624	The developed biocomposites	598:624	The developed biocomposites	598:624	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	1	13	theme	soft	298:301	arg1	surgery					287:293	reconstructive surgery	272:293	reconstructive surgery of soft and hard tissues	272:318	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	7	14	theme	porous	1358:1363	arg1	material					1365:1372	the applied porous material	1346:1372	the applied porous material	1346:1372	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	15	theme	perforated	1462:1471	arg1	implant					1500:1506	perforated metal or polymeric cranial implant	1462:1506	perforated metal or polymeric cranial implant is applied	1462:1517	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	16	theme	cranial	1492:1498	arg1	implant					1500:1506	perforated metal or polymeric cranial implant	1462:1506	perforated metal or polymeric cranial implant is applied	1462:1517	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	2	17	theme	perforated	407:416	arg1	stripes					423:429	perforated mesh stripes	407:429	perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	407:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	1	18	theme	bacterial	169:177	arg1	composites					189:198	new, stable bacterial cellulose composites	157:198	new, stable bacterial cellulose composites	157:198	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	2	19	theme	perpendicular	483:495	arg1	growth					497:502	perpendicular growth	483:502	perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	483:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	1	20	with	preparation	142:152	arg1	materials					222:230	perforated solid materials	205:230	perforated solid materials	205:230	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	5	21	theme	tensile	1090:1096	arg1	strength					1098:1105	its tensile strength	1086:1105	its tensile strength	1086:1105	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	1	22	theme	cellulose	179:187	arg1	composites					189:198	new, stable bacterial cellulose composites	157:198	new, stable bacterial cellulose composites	157:198	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	7	23	theme	great	1285:1289	arg1	potential					1291:1299	the great potential	1281:1299	the great potential of application in medicine	1281:1326	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	2	24	theme	nanocellulose	517:529	arg1	growth					497:502	perpendicular growth	483:502	perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	483:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	4	25	theme	cultivation	935:945	arg1	process					924:930	the process	920:930	the process of cultivation and purification	920:962	The obtained results indicate that the composites components are well integrated after the process of cultivation and purification.
30261126	7	26	contain	have	1276:1279	arg2	potential					1291:1299	the great potential	1281:1299	the great potential of application in medicine	1281:1326	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	26	contain	have	1276:1279	arg1	composites					1265:1274	the composites	1261:1274	the composites	1261:1274	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	27	theme	polymeric	1482:1490	arg1	implant					1500:1506	perforated metal or polymeric cranial implant	1462:1506	perforated metal or polymeric cranial implant is applied	1462:1517	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	5	28	theme	mechanical	1019:1028	arg1	properties					1030:1039	mechanical properties	1019:1039	mechanical properties of the polypropylene porous mesh	1019:1072	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	1	29	theme	composites	189:198	arg1	preparation					142:152	preparation	142:152	preparation of new, stable bacterial cellulose composites with perforated solid materials	142:230	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	0	30	theme	Stable	0:5	arg1	composite					7:15	Stable composite	0:15	Stable composite of bacterial nanocellulose	0:42	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	0	30	theme	Stable	0:5	arg1	nanocellulose					30:42	bacterial nanocellulose	20:42	bacterial nanocellulose	20:42	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	5	31	theme	polypropylene	1048:1060	arg1	mesh					1069:1072	the polypropylene porous mesh	1044:1072	the polypropylene porous mesh	1044:1072	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	5	32	theme	Bacterial	965:973	arg1	nanocellulose					975:987	Bacterial nanocellulose	965:987	Bacterial nanocellulose	965:987	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	0	33	theme	bacterial	20:28	arg1	nanocellulose					30:42	bacterial nanocellulose	20:42	bacterial nanocellulose	20:42	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	1	34	theme	hard	307:310	arg1	surgery					287:293	reconstructive surgery	272:293	reconstructive surgery of soft and hard tissues	272:318	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	5	35	theme	porous	1062:1067	arg1	mesh					1069:1072	the polypropylene porous mesh	1044:1072	the polypropylene porous mesh	1044:1072	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	1	36	theme	perforated	205:214	arg1	materials					222:230	perforated solid materials	205:230	perforated solid materials	205:230	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	2	37	theme	culture	458:464	arg1	medium					466:471	the culture medium	454:471	the culture medium	454:471	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	3	38	theme	mast	813:816	arg1	adhesion					823:830	mast cell adhesion	813:830	mast cell adhesion	813:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	1	39	dep	new	157:159	arg1	stable					162:167	stable	162:167	stable	162:167	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	1	40	theme	solid	216:220	arg1	materials					222:230	perforated solid materials	205:230	perforated solid materials	205:230	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	2	41	theme	bacterial	507:515	arg1	nanocellulose					517:529	bacterial nanocellulose	507:529	bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	507:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	3	42	theme	cell	818:821	arg1	adhesion					823:830	mast cell adhesion	813:830	mast cell adhesion	813:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	3	43	theme	in	703:704	arg1	responses					725:733	their in vitro inflammatory responses	697:733	their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion	697:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	6	44	theme	cellulose	1165:1173	arg1	application					1140:1150	application	1140:1150	application of bacterial cellulose	1140:1173	Moreover, application of bacterial cellulose makes the composites less immunogenic as compared to polypropylene itself.
30261126	0	45	theme	nanocellulose	30:42	arg1	mesh					73:76	perforated polypropylene mesh	48:76	perforated polypropylene mesh for biomedical applications	48:104	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	0	45	theme	nanocellulose	30:42	arg1	composite					7:15	Stable composite	0:15	Stable composite of bacterial nanocellulose	0:42	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	0	45	theme	nanocellulose	30:42	arg1	nanocellulose					30:42	bacterial nanocellulose	20:42	bacterial nanocellulose	20:42	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	7	46	dep	biocompatibility	1581:1596	arg1	improvement					1625:1635	improvement	1625:1635	improvement	1625:1635	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	47	from	barrier	1540:1546	arg1	application					1555:1565	any application	1551:1565	any application that requires biocompatibility or antiadhesive properties improvement	1551:1635	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	48	theme	hernia	1423:1428	arg1	mesh					1430:1433	porous hernia mesh	1416:1433	porous hernia mesh	1416:1433	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	3	49	theme	mechanical	661:670	arg1	strength					672:679	mechanical strength	661:679	mechanical strength	661:679	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	7	50	theme	application	1304:1314	arg1	potential					1291:1299	the great potential	1281:1299	the great potential of application in medicine	1281:1326	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	0	51	theme	polypropylene	59:71	arg1	mesh					73:76	perforated polypropylene mesh	48:76	perforated polypropylene mesh for biomedical applications	48:104	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	7	52	theme	antiadhesive	1601:1612	arg1	properties					1614:1623	antiadhesive properties	1601:1623	antiadhesive properties	1601:1623	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	53	used	used	1384:1387	arg2	composites					1265:1274	the composites	1261:1274	the composites	1261:1274	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	54	dep	implant	1500:1506	arg1	applied					1511:1517	applied	1511:1517	is applied	1508:1517	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	0	55	theme	perforated	48:57	arg1	mesh					73:76	perforated polypropylene mesh	48:76	perforated polypropylene mesh for biomedical applications	48:104	Stable composite of bacterial nanocellulose and perforated polypropylene mesh for biomedical applications.
30261126	2	56	theme	stripes	423:429	arg1	stripes					423:429	perforated mesh stripes	407:429	perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	407:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	2	56	theme	stripes	423:429	arg1	set					400:402	a set	398:402	a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	398:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	7	57	theme	porous	1416:1421	arg1	mesh					1430:1433	porous hernia mesh	1416:1433	porous hernia mesh	1416:1433	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	9	58	theme	Part	1688:1691	arg1	B					1693:1693	Part B	1688:1693	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.	1669:1729	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.
30261126	2	59	theme	designed	363:370	arg1	bioreactors					372:382	specially designed bioreactors	353:382	specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	353:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	1	60	dep	soft	298:301	arg1	tissues					312:318	tissues	312:318	tissues	312:318	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	7	61	dep	either	1389:1394	arg1	hernioplasty					1399:1410	hernioplasty	1399:1410	hernioplasty (if porous hernia mesh is used)	1399:1442	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	61	dep	either	1389:1394	arg1	cranioplasty					1445:1456	cranioplasty	1445:1456	cranioplasty (if perforated metal or polymeric cranial implant is applied)	1445:1518	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	62	used	used	1438:1441	arg2	mesh					1430:1433	porous hernia mesh	1416:1433	porous hernia mesh	1416:1433	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	63	theme	metal	1473:1477	arg1	implant					1500:1506	perforated metal or polymeric cranial implant	1462:1506	perforated metal or polymeric cranial implant is applied	1462:1517	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	1	64	theme	biomedical	236:245	arg1	applications					247:258	biomedical applications	236:258	biomedical applications	236:258	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	7	65	dep	used	1384:1387	arg1	barrier					1540:1546	a protective barrier	1527:1546	a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement	1527:1635	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	7	65	dep	used	1384:1387	arg1	either					1389:1394	either	1389:1394	either	1389:1394	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	8	66	dep	©	1638:1638	arg1	Inc.					1664:1667	Inc.	1664:1667	Inc.	1664:1667	© 2018 Wiley Periodicals, Inc.
30261126	1	67	theme	preparation	142:152	arg1	method					132:137	the method	128:137	the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications	128:258	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	3	68	theme	inflammatory	712:723	arg1	responses					725:733	their in vitro inflammatory responses	697:733	their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion	697:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	7	69	dep	hernioplasty	1399:1410	arg1	used					1438:1441	used	1438:1441	is used	1435:1441	Therefore, the composites have the great potential of application in medicine, and depending on the applied porous material, might be used either in hernioplasty (if porous hernia mesh is used), cranioplasty (if perforated metal or polymeric cranial implant is applied), or as a protective barrier in any application that requires biocompatibility or antiadhesive properties improvement.
30261126	6	70	theme	bacterial	1155:1163	arg1	cellulose					1165:1173	bacterial cellulose	1155:1173	bacterial cellulose	1155:1173	Moreover, application of bacterial cellulose makes the composites less immunogenic as compared to polypropylene itself.
30261126	5	71	theme	mesh	1069:1072	arg1	properties					1030:1039	mechanical properties	1019:1039	mechanical properties of the polypropylene porous mesh	1019:1072	Bacterial nanocellulose does not negatively influence mechanical properties of the polypropylene porous mesh, preserving its tensile strength, elasticity, and load.
30261126	2	72	theme	mesh	418:421	arg1	stripes					423:429	perforated mesh stripes	407:429	perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture	407:595	The composites were obtained in specially designed bioreactors equipped with a set of perforated mesh stripes threaded vertically to the culture medium, ensuring perpendicular growth of bacterial nanocellulose synthesized by Komagataeibacter xylinus E25 in stationary culture.
30261126	9	73	dep	Res	1684:1686	arg1	2019					1725:1728	2019	1725:1728	2019	1725:1728	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.
30261126	9	73	dep	Res	1684:1686	arg1	B					1693:1693	Part B	1688:1693	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.	1669:1729	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.
30261126	9	73	dep	Res	1684:1686	arg1	Biomater					1701:1708	Biomater	1701:1708	Biomater	1701:1708	J Biomed Mater Res Part B: Appl Biomater 107B: 978-987, 2019.
30261126	3	74	theme	cell	749:752	arg1	degranulation					754:766	mast cell degranulation	744:766	mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion	744:830	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30261126	4	75	theme	composites	872:881	arg1	components					883:892	the composites components	868:892	the composites components	868:892	The obtained results indicate that the composites components are well integrated after the process of cultivation and purification.
30261126	1	76	theme	new	157:159	arg1	composites					189:198	new, stable bacterial cellulose composites	157:198	new, stable bacterial cellulose composites	157:198	The article presents the method of preparation of new, stable bacterial cellulose composites with perforated solid materials for biomedical applications, comprising reconstructive surgery of soft and hard tissues.
30261126	3	77	dep	in	703:704	arg1	vitro					706:710	vitro	706:710	vitro	706:710	The developed biocomposites have been tested for stability and mechanical strength, as well as for their in vitro inflammatory responses shown as mast cell degranulation with N-acetyl-β-d-hexosaminidase release and mast cell adhesion.
30905753	4	0	theme	colour	782:787	arg1	values					789:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values	748:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	748:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	9	1	theme	FTIR	1484:1487	arg1	spectra					1489:1495	The FTIR spectra	1480:1495	The FTIR spectra of extruded samples	1480:1515	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	3	2	theme	0.01 g/100 g	484:495	arg1	fat					497:499	0.01 g/100 g fat	484:499	0.01 g/100 g fat	484:499	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	4	3	from	30.4 g/100 g.	653:665	arg1	treatments					629:638	treatments	629:638	treatments from 32.5 to 30.4 g/100 g.	629:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	3	from	30.4 g/100 g.	653:665	arg1	content					618:624	amylose content	610:624	amylose content of treatments from 32.5 to 30.4 g/100 g.	610:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	1	4	theme	%	238:238	arg1	contents					216:223	moisture contents	207:223	moisture contents of 16% and 18%	207:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	5	5	dep	significant	892:902	arg1	p ≤ 0.05					905:912	p ≤ 0.05	905:912	p ≤ 0.05	905:912	Swelling index, solubility index and light transmittance presented a significant (p ≤ 0.05) increase upon extrusion.
30905753	1	6	attach	isolated	148:155	arg2	Starch					141:146	Starch	141:146	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18%	141:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	1	6	attach	isolated	148:155	arg1	seeds					179:183	Desi Bengal gram seeds	162:183	Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18%	162:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	0	7	theme	Physico-chemical	0:15	arg1	properties					33:42	Physico-chemical and rheological properties	0:42	properties	33:42	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	1	8	theme	Desi	162:165	arg1	seeds					179:183	Desi Bengal gram seeds	162:183	Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18%	162:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	9	9	theme	stretches	1583:1591	arg1	intensities					1539:1549	intensities	1539:1549	intensities of hydroxyl, alkyl and carbonyl stretches after extrusion	1539:1607	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	4	10	theme	Extrusion	545:553	arg1	treatment					555:563	Extrusion treatment	545:563	Extrusion treatment	545:563	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	6	11	dep	18	1080:1081	arg1	to					1077:1078	to	1077:1078	to	1077:1078	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	1	12	theme	gram	174:177	arg1	seeds					179:183	Desi Bengal gram seeds	162:183	Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18%	162:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	0	13	theme	high	102:105	arg1	extrusion					130:138	high temperature short time extrusion	102:138	high temperature short time extrusion	102:138	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	4	14	from	content	618:624	arg1	30.4 g/100 g.					653:665	30.4 g/100 g.	653:665	30.4 g/100 g.	653:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	15	theme	treatments	629:638	arg1	content					618:624	amylose content	610:624	amylose content of treatments from 32.5 to 30.4 g/100 g.	610:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	0	16	theme	short	119:123	arg1	extrusion					130:138	high temperature short time extrusion	102:138	high temperature short time extrusion	102:138	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	8	17	theme	starches	1344:1351	arg1	G					1303:1303	G'	1303:1304	G'	1303:1304	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	8	17	theme	starches	1344:1351	arg1	modulus					1294:1300	The storage modulus	1282:1300	The storage modulus (G') of both untreated as well as treated starches	1282:1351	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	4	18	theme	Hunter	695:700	arg1	value					713:717	Hunter colour 'L' value (91.6-78.5)	695:729	Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	695:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	19	with	value	713:717	arg1	increase					736:743	increase	736:743	increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	736:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	7	20	theme	structure	1271:1279	arg1	loss					1254:1257	loss	1254:1257	loss of granular structure	1254:1279	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	5	21	theme	solubility	839:848	arg1	index					850:854	solubility index	839:854	solubility index	839:854	Swelling index, solubility index and light transmittance presented a significant (p ≤ 0.05) increase upon extrusion.
30905753	8	22	from	increase	1372:1379	arg1	frequency					1392:1400	angular frequency	1384:1400	angular frequency	1384:1400	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	6	23	theme	Pasting	940:946	arg1	properties					948:957	Pasting properties	940:957	Pasting properties of extruded treatments	940:980	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	3	24	theme	native	439:444	arg1	starch					446:451	native starch	439:451	native starch	439:451	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	7	25	theme	small	1153:1157	arg1	granules					1190:1197	small, spherical and smooth surfaced granules	1153:1197	small, spherical and smooth surfaced granules	1153:1197	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	1	26	theme	treatment	290:298	arg1	HTST					301:304	a high temperature short time extrusion treatment (HTST)	250:305	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	8	27	theme	angular	1384:1390	arg1	frequency					1392:1400	angular frequency	1384:1400	angular frequency	1384:1400	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	7	28	theme	starch	1137:1142	arg1	micrographs					1103:1113	Scanning electron micrographs	1085:1113	Scanning electron micrographs of native Bengal gram starch	1085:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	0	29	dep	Cicer	60:64	arg1	L.					76:77	Cicer arietinum L.	60:77	Cicer arietinum L.	60:77	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	0	30	theme	starch	80:85	arg1	properties					33:42	Physico-chemical and rheological properties	0:42	properties	33:42	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	2	31	theme	treated	383:389	arg1	starches					391:398	untreated and treated starches	369:398	untreated and treated starches	369:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	6	32	dep	decreased	982:990	arg1	p ≤ 0.05					1006:1013	p ≤ 0.05	1006:1013	p ≤ 0.05	1006:1013	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	6	33	from	%	1082:1082	arg1	increase					1036:1043	increase	1036:1043	increase in the moisture content from 16 to 18%	1036:1082	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	6	33	from	%	1082:1082	arg1	content					1061:1067	the moisture content	1048:1067	the moisture content from 16 to 18%	1048:1082	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	8	34	theme	storage	1286:1292	arg1	G					1303:1303	G'	1303:1304	G'	1303:1304	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	8	34	theme	storage	1286:1292	arg1	modulus					1294:1300	The storage modulus	1282:1300	The storage modulus (G') of both untreated as well as treated starches	1282:1351	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	2	35	theme	rheological	343:353	arg1	properties					355:364	Physicochemical, morphological and rheological properties	308:364	Physicochemical, morphological and rheological properties of untreated and treated starches	308:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	7	36	theme	electron	1094:1101	arg1	micrographs					1103:1113	Scanning electron micrographs	1085:1113	Scanning electron micrographs of native Bengal gram starch	1085:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	8	37	theme	elastic	1438:1444	arg1	behaviour					1446:1454	elastic behaviour	1438:1454	elastic behaviour	1438:1454	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	2	38	theme	starches	391:398	arg1	properties					355:364	Physicochemical, morphological and rheological properties	308:364	Physicochemical, morphological and rheological properties of untreated and treated starches	308:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	0	39	theme	rheological	21:31	arg1	properties					33:42	Physico-chemical and rheological properties	0:42	properties	33:42	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	7	40	dep	smooth	1174:1179	arg1	surfaced					1181:1188	surfaced	1181:1188	surfaced	1181:1188	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	6	41	theme	treatments	971:980	arg1	properties					948:957	Pasting properties	940:957	Pasting properties of extruded treatments	940:980	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	1	42	theme	temperature	257:267	arg1	HTST					301:304	a high temperature short time extrusion treatment (HTST)	250:305	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	0	43	theme	gram	54:57	arg1	starch					80:85	Bengal gram (Cicer arietinum L.) starch	47:85	Bengal gram (Cicer arietinum L.) starch	47:85	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	1	44	theme	time	275:278	arg1	HTST					301:304	a high temperature short time extrusion treatment (HTST)	250:305	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	9	45	theme	carbonyl	1574:1581	arg1	stretches					1583:1591	hydroxyl, alkyl and carbonyl stretches	1554:1591	stretches	1583:1591	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	6	46	theme	moisture	1052:1059	arg1	content					1061:1067	the moisture content	1048:1067	the moisture content from 16 to 18%	1048:1082	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	8	47	theme	untreated	1315:1323	arg1	starches					1344:1351	both untreated as well as treated starches	1310:1351	both untreated as well as treated starches	1310:1351	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	1	48	theme	moisture	207:214	arg1	contents					216:223	moisture contents	207:223	moisture contents of 16% and 18%	207:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	2	49	theme	morphological	325:337	arg1	properties					355:364	Physicochemical, morphological and rheological properties	308:364	Physicochemical, morphological and rheological properties of untreated and treated starches	308:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	7	50	theme	smooth	1174:1179	arg1	granules					1190:1197	small, spherical and smooth surfaced granules	1153:1197	small, spherical and smooth surfaced granules	1153:1197	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	2	51	theme	Physicochemical	308:322	arg1	properties					355:364	Physicochemical, morphological and rheological properties	308:364	Physicochemical, morphological and rheological properties of untreated and treated starches	308:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	1	52	theme	%	230:230	arg1	contents					216:223	moisture contents	207:223	moisture contents of 16% and 18%	207:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	9	53	theme	hydroxyl	1554:1561	arg1	stretches					1583:1591	hydroxyl, alkyl and carbonyl stretches	1554:1591	stretches	1583:1591	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	7	54	theme	spherical	1160:1168	arg1	granules					1190:1197	small, spherical and smooth surfaced granules	1153:1197	small, spherical and smooth surfaced granules	1153:1197	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	3	55	theme	0.25 g/100 g	523:534	arg1	protein					536:542	0.25 g/100 g protein	523:542	0.25 g/100 g protein	523:542	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	9	56	theme	alkyl	1564:1568	arg1	stretches					1583:1591	hydroxyl, alkyl and carbonyl stretches	1554:1591	stretches	1583:1591	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	4	57	theme	significant	574:584	arg1	p ≤ 0.05					597:604	p ≤ 0.05	597:604	p ≤ 0.05	597:604	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	57	theme	significant	574:584	arg1	reduction					586:594	a significant reduction	572:594	a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g.	572:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	58	theme	b	767:767	arg1	values					789:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values	748:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	748:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	1	59	theme	Bengal	167:172	arg1	seeds					179:183	Desi Bengal gram seeds	162:183	Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18%	162:238	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	0	60	theme	temperature	107:117	arg1	extrusion					130:138	high temperature short time extrusion	102:138	high temperature short time extrusion	102:138	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	9	61	theme	samples	1509:1515	arg1	spectra					1489:1495	The FTIR spectra	1480:1495	The FTIR spectra of extruded samples	1480:1515	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	8	62	theme	treated	1336:1342	arg1	starches					1344:1351	both untreated as well as treated starches	1310:1351	both untreated as well as treated starches	1310:1351	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	4	63	theme	amylose	610:616	arg1	content					618:624	amylose content	610:624	amylose content of treatments from 32.5 to 30.4 g/100 g.	610:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	0	64	theme	time	125:128	arg1	extrusion					130:138	high temperature short time extrusion	102:138	high temperature short time extrusion	102:138	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	7	65	theme	native	1118:1123	arg1	starch					1137:1142	native Bengal gram starch	1118:1142	native Bengal gram starch	1118:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	5	66	theme	Swelling	823:830	arg1	index					832:836	Swelling index	823:836	Swelling index	823:836	Swelling index, solubility index and light transmittance presented a significant (p ≤ 0.05) increase upon extrusion.
30905753	4	67	theme	samples	799:805	arg1	values					789:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values	748:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	748:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	68	theme	a	749:749	arg1	values					789:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values	748:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	748:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	69	theme	colour	702:707	arg1	value					713:717	Hunter colour 'L' value (91.6-78.5)	695:729	Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	695:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	7	70	theme	granular	1262:1269	arg1	structure					1271:1279	granular structure	1262:1279	granular structure	1262:1279	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	7	71	theme	Bengal	1125:1130	arg1	starch					1137:1142	native Bengal gram starch	1118:1142	native Bengal gram starch	1118:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	7	72	theme	gram	1132:1135	arg1	starch					1137:1142	native Bengal gram starch	1118:1142	native Bengal gram starch	1118:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	4	73	from	reduction	586:594	arg1	content					618:624	amylose content	610:624	amylose content of treatments from 32.5 to 30.4 g/100 g.	610:665	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	4	74	from	increase	736:743	arg1	values					789:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values	748:794	'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	748:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	5	75	theme	significant	892:902	arg1	increase					915:922	a significant (p ≤ 0.05) increase	890:922	a significant (p ≤ 0.05) increase upon extrusion	890:937	Swelling index, solubility index and light transmittance presented a significant (p ≤ 0.05) increase upon extrusion.
30905753	3	76	theme	Proximate	414:422	arg1	composition					424:434	Proximate composition	414:434	Proximate composition of native starch	414:451	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	7	77	theme	Scanning	1085:1092	arg1	micrographs					1103:1113	Scanning electron micrographs	1085:1113	Scanning electron micrographs of native Bengal gram starch	1085:1142	Scanning electron micrographs of native Bengal gram starch revealed small, spherical and smooth surfaced granules while granules were not visible after extrusion due to loss of granular structure.
30905753	9	78	from	reduction	1526:1534	arg1	intensities					1539:1549	intensities	1539:1549	intensities of hydroxyl, alkyl and carbonyl stretches after extrusion	1539:1607	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	8	79	theme	behaviour	1446:1454	arg1	dominance					1425:1433	the dominance	1421:1433	the dominance of elastic behaviour	1421:1454	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	4	80	theme	L'	710:711	arg1	value					713:717	Hunter colour 'L' value (91.6-78.5)	695:729	Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	695:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	5	81	theme	light	860:864	arg1	transmittance					866:878	light transmittance	860:878	light transmittance	860:878	Swelling index, solubility index and light transmittance presented a significant (p ≤ 0.05) increase upon extrusion.
30905753	8	82	theme	viscous	1461:1467	arg1	behaviour					1469:1477	viscous behaviour	1461:1477	viscous behaviour	1461:1477	The storage modulus (G') of both untreated as well as treated starches increased with the increase in angular frequency, thereby depicting the dominance of elastic behaviour over viscous behaviour.
30905753	3	83	theme	starch	446:451	arg1	composition					424:434	Proximate composition	414:434	Proximate composition of native starch	414:451	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	0	84	theme	Bengal	47:52	arg1	starch					80:85	Bengal gram (Cicer arietinum L.) starch	47:85	Bengal gram (Cicer arietinum L.) starch	47:85	Physico-chemical and rheological properties of Bengal gram (Cicer arietinum L.) starch as affected by high temperature short time extrusion.
30905753	1	85	theme	high	252:255	arg1	temperature					257:267	a high temperature	250:267	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	6	86	theme	extruded	962:969	arg1	treatments					971:980	extruded treatments	962:980	extruded treatments	962:980	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	2	87	theme	untreated	369:377	arg1	starches					391:398	untreated and treated starches	369:398	untreated and treated starches	369:398	Physicochemical, morphological and rheological properties of untreated and treated starches were studied.
30905753	1	88	theme	short	269:273	arg1	HTST					301:304	a high temperature short time extrusion treatment (HTST)	250:305	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	9	89	theme	extruded	1500:1507	arg1	samples					1509:1515	extruded samples	1500:1515	extruded samples	1500:1515	The FTIR spectra of extruded samples revealed reduction in intensities of hydroxyl, alkyl and carbonyl stretches after extrusion.
30905753	1	90	theme	extrusion	280:288	arg1	HTST					301:304	a high temperature short time extrusion treatment (HTST)	250:305	a high temperature short time extrusion treatment (HTST)	250:305	Starch isolated from Desi Bengal gram seeds after conditioning to moisture contents of 16% and 18% was given a high temperature short time extrusion treatment (HTST).
30905753	4	91	dep	30.4 g/100 g.	653:665	arg1	to					650:651	to	650:651	to	650:651	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
30905753	3	92	theme	9.2 g/100 g	462:472	arg1	moisture					474:481	9.2 g/100 g moisture	462:481	9.2 g/100 g moisture	462:481	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	6	93	from	increase	1036:1043	arg1	%					1082:1082	16 to 18%	1074:1082	16 to 18%	1074:1082	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	6	93	from	increase	1036:1043	arg1	content					1061:1067	the moisture content	1048:1067	the moisture content from 16 to 18%	1048:1082	Pasting properties of extruded treatments decreased significantly(p ≤ 0.05) upon extrusion with increase in the moisture content from 16 to 18%.
30905753	3	94	theme	0.22 g/100 g	502:513	arg1	ash					515:517	0.22 g/100 g ash	502:517	0.22 g/100 g ash	502:517	Proximate composition of native starch revealed 9.2 g/100 g moisture, 0.01 g/100 g fat, 0.22 g/100 g ash and 0.25 g/100 g protein.
30905753	4	95	from	reduction	682:690	arg1	value					713:717	Hunter colour 'L' value (91.6-78.5)	695:729	Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion	695:820	Extrusion treatment led to a significant reduction (p ≤ 0.05) in amylose content of treatments from 32.5 to 30.4 g/100 g. Also there was reduction in Hunter colour 'L' value (91.6-78.5) with increase in 'a' (0.0-0.4) and 'b' (10.8-14.8) colour values of samples upon extrusion.
29588205	5	0	theme	cell	1081:1084	arg1	RPE1					1092:1095	RPE1	1092:1095	RPE1	1092:1095	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	0	theme	cell	1081:1084	arg1	line					1086:1089	normal human retina cell line	1061:1089	normal human retina cell line (RPE1)	1061:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	1	1	dep	new	92:94	arg1	biocompatible					96:108	biocompatible	96:108	biocompatible	96:108	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	4	2	theme	surface	851:857	arg1	morphology					859:868	surface morphology	851:868	surface morphology	851:868	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	1	3	dep	biocompatible	96:108	arg1	biodegradable					111:123	biodegradable	111:123	biodegradable	111:123	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	2	4	theme	succinic	487:494	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	4	theme	succinic	487:494	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	3	5	theme	elemental	780:788	arg1	analyses					790:797	elemental analyses	780:797	elemental analyses	780:797	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	3	5	theme	elemental	780:788	arg1	spectroscopy					814:825	13C NMR spectroscopy	806:825	13C NMR spectroscopy	806:825	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	3	5	theme	elemental	780:788	arg1	FTIR					800:803	FTIR	800:803	FTIR	800:803	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	2	6	theme	sulfonic	539:546	arg1	CAPSA					555:559	CAPSA	555:559	CAPSA	555:559	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	6	theme	sulfonic	539:546	arg1	acids					548:552	3(celluloseamino) propane sulfonic acids	513:552	3(celluloseamino) propane sulfonic acids (CAPSA)	513:560	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	7	theme	aminoalkyl	717:726	arg1	derivative					733:742	the proper aminoalkyl acid derivative	706:742	the proper aminoalkyl acid derivative	706:742	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	8	with	esterification	621:634	arg1	chloride					647:654	tosyl chloride	641:654	tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative	641:742	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	9	theme	microcrystalline	584:599	arg1	MCC					612:614	MCC	612:614	MCC	612:614	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	9	theme	microcrystalline	584:599	arg1	cellulose					601:609	microcrystalline cellulose	584:609	microcrystalline cellulose (MCC)	584:615	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	10	theme	proper	710:715	arg1	derivative					733:742	the proper aminoalkyl acid derivative	706:742	the proper aminoalkyl acid derivative	706:742	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	1	11	theme	tissue	183:188	arg1	regeneration					190:201	tissue regeneration	183:201	tissue regeneration	183:201	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	2	12	theme	acid	457:460	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	12	theme	acid	457:460	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	5	13	theme	normal	1061:1066	arg1	RPE1					1092:1095	RPE1	1092:1095	RPE1	1092:1095	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	13	theme	normal	1061:1066	arg1	line					1086:1089	normal human retina cell line	1061:1089	normal human retina cell line (RPE1)	1061:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	2	14	theme	water-soluble	387:399	arg1	derivatives					411:421	three water-soluble cellulose derivatives	381:421	three water-soluble cellulose derivatives	381:421	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	15	theme	CABA	463:466	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	15	theme	CABA	463:466	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	5	16	theme	human	1068:1072	arg1	RPE1					1092:1095	RPE1	1092:1095	RPE1	1092:1095	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	16	theme	human	1068:1072	arg1	line					1086:1089	normal human retina cell line	1061:1089	normal human retina cell line (RPE1)	1061:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	17	dep	in	1036:1037	arg1	vitro					1039:1043	vitro	1039:1043	vitro	1039:1043	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	1	18	theme	new	92:94	arg1	carriers					214:221	carriers	214:221	carriers for different bioactive molecules such as drugs, proteins, and enzymes	214:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	18	theme	new	92:94	arg1	biopolymers					140:150	new biocompatible, biodegradable functionalized biopolymers	92:150	new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes	92:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	0	19	theme	Rational	0:7	arg1	design					9:14	Rational design	0:14	Rational design of novel water-soluble ampholytic cellulose derivatives	0:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	2	20	theme	celluloseamino	433:446	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	20	theme	celluloseamino	433:446	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	4	21	theme	new	908:910	arg1	biopolymers					923:933	the new ampholytic biopolymers	904:933	the new ampholytic biopolymers	904:933	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	4	22	theme	elemental	879:887	arg1	composition					889:899	the elemental composition	875:899	the elemental composition	875:899	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	0	23	theme	ampholytic	39:48	arg1	derivatives					60:70	novel water-soluble ampholytic cellulose derivatives	19:70	novel water-soluble ampholytic cellulose derivatives	19:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	2	24	theme	acid	728:731	arg1	derivative					733:742	the proper aminoalkyl acid derivative	706:742	the proper aminoalkyl acid derivative	706:742	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	25	theme	cellulose	401:409	arg1	derivatives					411:421	three water-soluble cellulose derivatives	381:421	three water-soluble cellulose derivatives	381:421	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	1	26	theme	continuous	304:313	arg1	challenge					315:323	a continuous challenge	302:323	a continuous challenge that need to be extensively explored	302:360	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	0	27	theme	water-soluble	25:37	arg1	derivatives					60:70	novel water-soluble ampholytic cellulose derivatives	19:70	novel water-soluble ampholytic cellulose derivatives	19:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	5	28	theme	in	1036:1037	arg1	cytotoxicity					1045:1056	their in vitro cytotoxicity	1030:1056	their in vitro cytotoxicity on normal human retina cell line (RPE1)	1030:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	0	29	theme	novel	19:23	arg1	derivatives					60:70	novel water-soluble ampholytic cellulose derivatives	19:70	novel water-soluble ampholytic cellulose derivatives	19:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	1	30	theme	different	227:235	arg1	proteins					272:279	proteins	272:279	proteins	272:279	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	30	theme	different	227:235	arg1	molecules					247:255	different bioactive molecules	227:255	different bioactive molecules such as drugs, proteins, and enzymes	227:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	30	theme	different	227:235	arg1	drugs					265:269	drugs	265:269	drugs	265:269	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	30	theme	different	227:235	arg1	enzymes					286:292	enzymes	286:292	enzymes	286:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	0	31	theme	derivatives	60:70	arg1	design					9:14	Rational design	0:14	Rational design of novel water-soluble ampholytic cellulose derivatives	0:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	2	32	theme	propane	531:537	arg1	CAPSA					555:559	CAPSA	555:559	CAPSA	555:559	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	32	theme	propane	531:537	arg1	acids					548:552	3(celluloseamino) propane sulfonic acids	513:552	3(celluloseamino) propane sulfonic acids (CAPSA)	513:560	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	4	33	theme	biopolymers	923:933	arg1	morphology					859:868	surface morphology	851:868	surface morphology	851:868	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	4	33	theme	biopolymers	923:933	arg1	composition					889:899	the elemental composition	875:899	the elemental composition	875:899	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	4	33	theme	biopolymers	923:933	arg1	stability					840:848	The thermal stability	828:848	The thermal stability	828:848	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	5	34	theme	new	974:976	arg1	derivatives					999:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	1	35	theme	functionalized	125:138	arg1	carriers					214:221	carriers	214:221	carriers for different bioactive molecules such as drugs, proteins, and enzymes	214:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	35	theme	functionalized	125:138	arg1	biopolymers					140:150	new biocompatible, biodegradable functionalized biopolymers	92:150	new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes	92:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	0	36	theme	cellulose	50:58	arg1	derivatives					60:70	novel water-soluble ampholytic cellulose derivatives	19:70	novel water-soluble ampholytic cellulose derivatives	19:70	Rational design of novel water-soluble ampholytic cellulose derivatives.
29588205	5	37	theme	cellulose	989:997	arg1	derivatives					999:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	38	theme	ampholytic	978:987	arg1	derivatives					999:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives	970:1009	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	1	39	theme	biopolymers	140:150	arg1	development					77:87	The development	73:87	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes	73:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	3	40	theme	NMR	810:812	arg1	analyses					790:797	elemental analyses	780:797	elemental analyses	780:797	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	3	40	theme	NMR	810:812	arg1	spectroscopy					814:825	13C NMR spectroscopy	806:825	13C NMR spectroscopy	806:825	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	5	41	theme	MTT	1101:1103	arg1	assay					1105:1109	MTT assay	1101:1109	MTT assay	1101:1109	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	2	42	theme	celluloseamino	515:528	arg1	CAPSA					555:559	CAPSA	555:559	CAPSA	555:559	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	42	theme	celluloseamino	515:528	arg1	acids					548:552	3(celluloseamino) propane sulfonic acids	513:552	3(celluloseamino) propane sulfonic acids (CAPSA)	513:560	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	43	theme	tosyl	641:645	arg1	chloride					647:654	tosyl chloride	641:654	tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative	641:742	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	1	44	theme	bioactive	237:245	arg1	proteins					272:279	proteins	272:279	proteins	272:279	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	44	theme	bioactive	237:245	arg1	molecules					247:255	different bioactive molecules	227:255	different bioactive molecules such as drugs, proteins, and enzymes	227:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	44	theme	bioactive	237:245	arg1	drugs					265:269	drugs	265:269	drugs	265:269	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	1	44	theme	bioactive	237:245	arg1	enzymes					286:292	enzymes	286:292	enzymes	286:292	The development of new biocompatible, biodegradable functionalized biopolymers that can serve as scaffold for tissue regeneration or work as carriers for different bioactive molecules such as drugs, proteins, and enzymes remains a continuous challenge that need to be extensively explored.
29588205	4	45	theme	thermal	832:838	arg1	stability					840:848	The thermal stability	828:848	The thermal stability	828:848	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
29588205	5	46	from	cytotoxicity	1045:1056	arg1	RPE1					1092:1095	RPE1	1092:1095	RPE1	1092:1095	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	46	from	cytotoxicity	1045:1056	arg1	line					1086:1089	normal human retina cell line	1061:1089	normal human retina cell line (RPE1)	1061:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	2	47	theme	nucleophilic	677:688	arg1	substitution					690:701	nucleophilic substitution	677:701	nucleophilic substitution	677:701	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	3	48	theme	13C	806:808	arg1	analyses					790:797	elemental analyses	780:797	elemental analyses	780:797	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	3	48	theme	13C	806:808	arg1	spectroscopy					814:825	13C NMR spectroscopy	806:825	13C NMR spectroscopy	806:825	The products were characterized by elemental analyses, FTIR, 13C NMR spectroscopy.
29588205	2	49	theme	celluloseamino	471:484	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	49	theme	celluloseamino	471:484	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	5	50	theme	retina	1074:1079	arg1	RPE1					1092:1095	RPE1	1092:1095	RPE1	1092:1095	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	5	50	theme	retina	1074:1079	arg1	line					1086:1089	normal human retina cell line	1061:1089	normal human retina cell line (RPE1)	1061:1096	The new ampholytic cellulose derivatives were evaluated for their in vitro cytotoxicity on normal human retina cell line (RPE1) by MTT assay.
29588205	2	51	theme	butyric	449:455	arg1	acid					496:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid	424:499	namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA)	424:506	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	2	51	theme	butyric	449:455	arg1	CASA					502:505	CASA	502:505	CASA	502:505	For this purpose, three water-soluble cellulose derivatives; namely 4(celluloseamino) butyric acid (CABA) 2(celluloseamino) succinic acid (CASA), and 3(celluloseamino) propane sulfonic acids (CAPSA) were synthesized from microcrystalline cellulose (MCC) via esterification with tosyl chloride that was followed by nucleophilic substitution by the proper aminoalkyl acid derivative.
29588205	4	52	theme	ampholytic	912:921	arg1	biopolymers					923:933	the new ampholytic biopolymers	904:933	the new ampholytic biopolymers	904:933	The thermal stability, surface morphology, and the elemental composition of the new ampholytic biopolymers were also studied by TGA, EDX-SEM.
31873808	9	0	from	interface	1572:1580	arg1	concentration					1540:1552	protein concentration	1532:1552	protein concentration at the air-liquid interface	1532:1580	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	6	1	theme	surface	1067:1073	arg1	concentration					1075:1087	protein surface concentration	1059:1087	protein surface concentration	1059:1087	XPS indicated sucrose and trehalose reduced protein surface concentration better than dextran-containing formulations.
31873808	0	2	theme	Spray	80:84	arg1	Drying					86:91	Spray Drying	80:91	Spray Drying	80:91	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	5	3	dep	RESULTS	851:857	arg1	exhibited					887:895	exhibited	887:895	exhibited	887:895	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	1	4	dep	PURPOSE	94:100	arg1	is					124:125	is	124:125	is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity	124:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	8	5	with	formulations	1375:1386	arg1	excipients					1415:1424	saccharide-containing excipients	1393:1424	saccharide-containing excipients	1393:1424	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	1	6	theme	matrix	261:266	arg1	heterogeneity					268:280	their matrix heterogeneity	255:280	their matrix heterogeneity	255:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	6	7	theme	dextran-containing	1101:1118	arg1	formulations					1120:1131	dextran-containing formulations	1101:1131	dextran-containing formulations	1101:1131	XPS indicated sucrose and trehalose reduced protein surface concentration better than dextran-containing formulations.
31873808	1	8	from	effects	144:150	arg1	composition					203:213	the surface composition	191:213	the surface composition of spray-dried protein formulations and their matrix heterogeneity	191:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	4	9	theme	solid-state	776:786	arg1	exchange					807:814	solid-state hydrogen/deuterium exchange	776:814	solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS)	776:848	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	3	10	theme	exclusion	570:578	arg1	SEC					596:598	SEC	596:598	SEC	596:598	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	10	theme	exclusion	570:578	arg1	chromatography					580:593	size exclusion chromatography	565:593	size exclusion chromatography (SEC)	565:599	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	1	11	theme	heterogeneity	268:280	arg1	composition					203:213	the surface composition	191:213	the surface composition of spray-dried protein formulations and their matrix heterogeneity	191:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	9	12	theme	particle	1504:1511	arg1	surface					1513:1519	the particle surface	1500:1519	the particle surface	1500:1519	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	5	13	theme	excipients	929:938	arg1	inclusion					906:914	the inclusion	902:914	the inclusion of different excipients	902:938	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	8	14	theme	saccharide-containing	1393:1413	arg1	excipients					1415:1424	saccharide-containing excipients	1393:1424	saccharide-containing excipients	1393:1424	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	8	15	theme	Linear	1269:1274	arg1	correlation					1276:1286	Linear correlation	1269:1286	Linear correlation	1269:1286	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	3	16	theme	infrared	492:499	arg1	ssFTIR					515:520	ssFTIR	515:520	ssFTIR	515:520	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	16	theme	infrared	492:499	arg1	spectroscopy					501:512	solid-state Fourier-transform infrared spectroscopy	462:512	solid-state Fourier-transform infrared spectroscopy (ssFTIR)	462:521	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	2	17	theme	Spray-dried	291:301	arg1	formulations					303:314	METHODS Spray-dried formulations	283:314	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA)	283:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	8	18	theme	peak	1344:1347	arg1	area					1349:1352	ssHDX-MS peak area	1335:1352	ssHDX-MS peak area (R2 = 0.853)	1335:1365	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	8	18	theme	peak	1344:1347	arg1	R2 = 0.853					1355:1364	R2 = 0.853	1355:1364	R2 = 0.853	1355:1364	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	9	19	theme	Lower	1439:1443	arg1	species					1462:1468	Lower molecular weight species	1439:1468	CONCLUSIONS Lower molecular weight species of saccharides	1427:1483	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	7	20	theme	populations	1192:1202	arg1	populations					1192:1202	populations	1192:1202	populations	1192:1202	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	20	theme	populations	1192:1202	arg1	amount					1154:1159	the amount	1150:1159	the amount of deuterium incorporation and populations present	1150:1210	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	20	theme	populations	1192:1202	arg1	incorporation					1174:1186	deuterium incorporation	1164:1186	deuterium incorporation	1164:1186	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	20	theme	populations	1192:1202	arg1	largest					1221:1227	largest	1221:1227	largest	1221:1227	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	2	21	theme	METHODS	283:289	arg1	formulations					303:314	METHODS Spray-dried formulations	283:314	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA)	283:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	1	22	theme	saccharide-containing	155:175	arg1	excipients					177:186	saccharide-containing excipients	155:186	saccharide-containing excipients	155:186	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	9	23	theme	molecular	1445:1453	arg1	species					1462:1468	Lower molecular weight species	1439:1468	CONCLUSIONS Lower molecular weight species of saccharides	1427:1483	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	7	24	theme	deuterium	1164:1172	arg1	incorporation					1174:1186	deuterium incorporation	1164:1186	deuterium incorporation	1164:1186	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	9	25	theme	weight	1455:1460	arg1	species					1462:1468	Lower molecular weight species	1439:1468	CONCLUSIONS Lower molecular weight species of saccharides	1427:1483	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	2	26	theme	serum	339:343	arg1	BSA					354:356	BSA	354:356	BSA	354:356	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	2	26	theme	serum	339:343	arg1	albumin					345:351	bovine serum albumin	332:351	bovine serum albumin (BSA)	332:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	1	27	theme	excipients	177:186	arg1	effects					144:150	the effects	140:150	the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity	140:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	0	28	theme	Surface	0:6	arg1	Composition					8:18	Surface Composition	0:18	Surface Composition	0:18	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	4	29	with	exchange	807:814	arg1	spectrometry					826:837	mass spectrometry	821:837	mass spectrometry (ssHDX-MS)	821:848	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	4	29	with	exchange	807:814	arg1	ssHDX-MS					840:847	ssHDX-MS	840:847	ssHDX-MS	840:847	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	7	30	theme	incorporation	1174:1186	arg1	populations					1192:1202	populations	1192:1202	populations	1192:1202	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	30	theme	incorporation	1174:1186	arg1	amount					1154:1159	the amount	1150:1159	the amount of deuterium incorporation and populations present	1150:1210	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	30	theme	incorporation	1174:1186	arg1	incorporation					1174:1186	deuterium incorporation	1164:1186	deuterium incorporation	1164:1186	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	7	30	theme	incorporation	1174:1186	arg1	largest					1221:1227	largest	1221:1227	largest	1221:1227	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	2	31	theme	bovine	332:337	arg1	BSA					354:356	BSA	354:356	BSA	354:356	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	2	31	theme	bovine	332:337	arg1	albumin					345:351	bovine serum albumin	332:351	bovine serum albumin (BSA)	332:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	0	32	theme	Formulation	24:34	arg1	Heterogeneity					36:48	Formulation Heterogeneity	24:48	Formulation Heterogeneity	24:48	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	9	33	theme	reduced	1596:1602	arg1	heterogeneity					1615:1627	reduced population heterogeneity	1596:1627	reduced population heterogeneity	1596:1627	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	9	34	theme	air-liquid	1561:1570	arg1	interface					1572:1580	the air-liquid interface	1557:1580	the air-liquid interface	1557:1580	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	3	35	theme	size	565:568	arg1	SEC					596:598	SEC	596:598	SEC	596:598	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	35	theme	size	565:568	arg1	chromatography					580:593	size exclusion chromatography	565:593	size exclusion chromatography (SEC)	565:599	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	36	theme	Fourier-transform	474:490	arg1	ssFTIR					515:520	ssFTIR	515:520	ssFTIR	515:520	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	36	theme	Fourier-transform	474:490	arg1	spectroscopy					501:512	solid-state Fourier-transform infrared spectroscopy	462:512	solid-state Fourier-transform infrared spectroscopy (ssFTIR)	462:521	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	4	37	theme	conformational	730:743	arg1	differences					745:755	conformational differences	730:755	conformational differences	730:755	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	5	38	theme	secondary	994:1002	arg1	structure					1004:1012	secondary structure	994:1012	secondary structure	994:1012	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	2	39	theme	myoglobin	319:327	arg1	formulations					303:314	METHODS Spray-dried formulations	283:314	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA)	283:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	1	40	theme	surface	195:201	arg1	composition					203:213	the surface composition	191:213	the surface composition of spray-dried protein formulations and their matrix heterogeneity	191:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	8	41	theme	surface	1314:1320	arg1	coverage					1322:1329	protein surface coverage	1306:1329	protein surface coverage	1306:1329	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	9	42	theme	saccharides	1473:1483	arg1	species					1462:1468	Lower molecular weight species	1439:1468	CONCLUSIONS Lower molecular weight species of saccharides	1427:1483	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	9	43	theme	population	1604:1613	arg1	heterogeneity					1615:1627	reduced population heterogeneity	1596:1627	reduced population heterogeneity	1596:1627	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	4	44	theme	surface	649:655	arg1	coverage					657:664	Protein surface coverage	641:664	Protein surface coverage	641:664	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	4	45	theme	photoelectron	690:702	arg1	spectroscopy					704:715	X-ray photoelectron spectroscopy	684:715	X-ray photoelectron spectroscopy (XPS)	684:721	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	4	45	theme	photoelectron	690:702	arg1	XPS					718:720	XPS	718:720	XPS	718:720	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	6	46	theme	protein	1059:1065	arg1	concentration					1075:1087	protein surface concentration	1059:1087	protein surface concentration	1059:1087	XPS indicated sucrose and trehalose reduced protein surface concentration better than dextran-containing formulations.
31873808	3	47	theme	scanning	605:612	arg1	microscopy					623:632	scanning electron microscopy	605:632	scanning electron microscopy (SEM)	605:638	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	47	theme	scanning	605:612	arg1	SEM					635:637	SEM	635:637	SEM	635:637	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	9	48	theme	protein	1532:1538	arg1	concentration					1540:1552	protein concentration	1532:1552	protein concentration at the air-liquid interface	1532:1580	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	3	49	theme	solid-state	462:472	arg1	ssFTIR					515:520	ssFTIR	515:520	ssFTIR	515:520	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	49	theme	solid-state	462:472	arg1	spectroscopy					501:512	solid-state Fourier-transform infrared spectroscopy	462:512	solid-state Fourier-transform infrared spectroscopy (ssFTIR)	462:521	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	0	50	theme	Solids	61:66	arg1	Composition					8:18	Surface Composition	0:18	Surface Composition	0:18	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	0	50	theme	Solids	61:66	arg1	Heterogeneity					36:48	Formulation Heterogeneity	24:48	Formulation Heterogeneity	24:48	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	5	51	theme	different	919:927	arg1	excipients					929:938	different excipients	919:938	different excipients	919:938	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	8	52	theme	protein	1306:1312	arg1	coverage					1322:1329	protein surface coverage	1306:1329	protein surface coverage	1306:1329	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	7	53	dep	incorporation	1174:1186	arg1	present					1204:1210	present	1204:1210	present	1204:1210	Using ssHDX-MS, the amount of deuterium incorporation and populations present were the largest in the samples processed with dextrans.
31873808	5	54	theme	dextran	946:952	arg1	formulations					954:965	dextran formulations	946:965	dextran formulations indicating perturbation of secondary structure	946:1012	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	0	55	theme	Protein	53:59	arg1	Solids					61:66	Protein Solids	53:66	Protein Solids	53:66	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	9	56	theme	physical	1642:1649	arg1	stability					1651:1659	improved physical stability	1633:1659	improved physical stability	1633:1659	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	3	57	theme	differential	524:535	arg1	DSC					559:561	DSC	559:561	DSC	559:561	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	57	theme	differential	524:535	arg1	calorimetry					546:556	differential scanning calorimetry	524:556	differential scanning calorimetry (DSC)	524:562	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	9	58	theme	improved	1633:1640	arg1	stability					1651:1659	improved physical stability	1633:1659	improved physical stability	1633:1659	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	9	59	dep	CONCLUSIONS	1427:1437	arg1	species					1462:1468	Lower molecular weight species	1439:1468	CONCLUSIONS Lower molecular weight species of saccharides	1427:1483	CONCLUSIONS Lower molecular weight species of saccharides tend to enrich the particle surface and reduce protein concentration at the air-liquid interface, resulting in reduced population heterogeneity and improved physical stability, as identified by ssHDX-MS.
31873808	4	60	theme	mass	821:824	arg1	spectrometry					826:837	mass spectrometry	821:837	mass spectrometry (ssHDX-MS)	821:848	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	4	60	theme	mass	821:824	arg1	ssHDX-MS					840:847	ssHDX-MS	840:847	ssHDX-MS	840:847	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	0	61	gly	Heterogeneity	36:48	arg1	Solids					61:66	Protein Solids	53:66	Protein Solids	53:66	Surface Composition and Formulation Heterogeneity of Protein Solids Produced by Spray Drying.
31873808	1	62	theme	study	118:122	arg1	aim					106:108	The aim	102:108	The aim of this study	102:122	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	5	63	theme	structure	1004:1012	arg1	perturbation					978:989	perturbation	978:989	perturbation of secondary structure	978:1012	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	1	64	theme	spray-dried	218:228	arg1	formulations					238:249	spray-dried protein formulations	218:249	spray-dried protein formulations	218:249	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	4	65	theme	hydrogen/deuterium	788:805	arg1	exchange					807:814	solid-state hydrogen/deuterium exchange	776:814	solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS)	776:848	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	2	66	theme	albumin	345:351	arg1	formulations					303:314	METHODS Spray-dried formulations	283:314	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA)	283:357	METHODS Spray-dried formulations of myoglobin or bovine serum albumin (BSA) were prepared without excipient or with sucrose, trehalose, or dextrans.
31873808	1	67	theme	protein	230:236	arg1	formulations					238:249	spray-dried protein formulations	218:249	spray-dried protein formulations	218:249	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	4	68	theme	Protein	641:647	arg1	coverage					657:664	Protein surface coverage	641:664	Protein surface coverage	641:664	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	3	69	theme	electron	614:621	arg1	microscopy					623:632	scanning electron microscopy	605:632	scanning electron microscopy (SEM)	605:638	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	69	theme	electron	614:621	arg1	SEM					635:637	SEM	635:637	SEM	635:637	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	5	70	theme	Structural	859:868	arg1	differences					870:880	Structural differences	859:880	Structural differences	859:880	RESULTS Structural differences were exhibited with the inclusion of different excipients, with dextran formulations indicating perturbation of secondary structure.
31873808	3	71	theme	scanning	537:544	arg1	DSC					559:561	DSC	559:561	DSC	559:561	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	3	71	theme	scanning	537:544	arg1	calorimetry					546:556	differential scanning calorimetry	524:556	differential scanning calorimetry (DSC)	524:562	Samples were characterized by solid-state Fourier-transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC) and scanning electron microscopy (SEM).
31873808	8	72	theme	ssHDX-MS	1335:1342	arg1	area					1349:1352	ssHDX-MS peak area	1335:1352	ssHDX-MS peak area (R2 = 0.853)	1335:1365	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	8	72	theme	ssHDX-MS	1335:1342	arg1	R2 = 0.853					1355:1364	R2 = 0.853	1355:1364	R2 = 0.853	1355:1364	Linear correlation was found between protein surface coverage and ssHDX-MS peak area (R2 = 0.853) for all formulations with saccharide-containing excipients.
31873808	1	73	theme	formulations	238:249	arg1	composition					203:213	the surface composition	191:213	the surface composition of spray-dried protein formulations and their matrix heterogeneity	191:280	PURPOSE The aim of this study is to determine the effects of saccharide-containing excipients on the surface composition of spray-dried protein formulations and their matrix heterogeneity.
31873808	4	74	theme	X-ray	684:688	arg1	spectroscopy					704:715	X-ray photoelectron spectroscopy	684:715	X-ray photoelectron spectroscopy (XPS)	684:721	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
31873808	4	74	theme	X-ray	684:688	arg1	XPS					718:720	XPS	718:720	XPS	718:720	Protein surface coverage was determined by X-ray photoelectron spectroscopy (XPS), while conformational differences were determined by solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS).
30510200	4	0	theme	respiratory	702:712	arg1	hemocyanin					723:732	hemocyanin	723:732	hemocyanin	723:732	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	0	theme	respiratory	702:712	arg1	abundant					738:745	abundant	738:745	abundant	738:745	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	0	theme	respiratory	702:712	arg1	protein					714:720	the limnoriid respiratory protein	688:720	the limnoriid respiratory protein	688:720	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	1	with	incubation	791:800	arg1	hemocyanin					815:824	hemocyanin	815:824	hemocyanin	815:824	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	2	theme	limnoriid	692:700	arg1	hemocyanin					723:732	hemocyanin	723:732	hemocyanin	723:732	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	2	theme	limnoriid	692:700	arg1	abundant					738:745	abundant	738:745	abundant	738:745	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	2	theme	limnoriid	692:700	arg1	protein					714:720	the limnoriid respiratory protein	688:720	the limnoriid respiratory protein	688:720	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	1	3	from	polysaccharides	165:179	arg1	rich					157:160	rich	157:160	rich	157:160	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	3	4	dep	polysaccharides	496:510	arg1	glucomannans					540:551	glucomannans	540:551	glucomannans	540:551	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	3	4	dep	polysaccharides	496:510	arg1	polysaccharides					496:510	hexose-containing polysaccharides	478:510	hexose-containing polysaccharides (mainly cellulose, and also glucomannans)	478:552	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	3	4	dep	polysaccharides	496:510	arg1	cellulose					520:528	cellulose	520:528	cellulose	520:528	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	1	5	theme	Woody	83:87	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	5	theme	Woody	83:87	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	6	from	rich	157:160	arg1	polysaccharides					165:179	polysaccharides	165:179	polysaccharides that are bound into an insoluble fiber composite with lignin	165:240	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	2	7	theme	Limnoria	285:292	arg1	woodborers					261:270	Marine crustacean woodborers	243:270	Marine crustacean woodborers of the genus Limnoria	243:292	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	3	8	theme	fecal	433:437	arg1	pellets					439:445	fecal pellets	433:445	fecal pellets	433:445	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	1	9	theme	lignocellulosic	90:104	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	9	theme	lignocellulosic	90:104	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	3	10	theme	pellets	439:445	arg1	Analysis					421:428	Analysis	421:428	Analysis of fecal pellets	421:445	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	4	11	from	abundant	738:745	arg1	hindgut					754:760	the hindgut	750:760	the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases	750:874	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	1	12	theme	plant	107:111	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	12	theme	plant	107:111	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	2	13	theme	material	368:375	arg1	diet					342:345	a diet	340:345	a diet of this recalcitrant material	340:375	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	4	14	from	hindgut	754:760	arg1	abundant					738:745	abundant	738:745	abundant	738:745	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	4	14	from	hindgut	754:760	arg1	protein					714:720	the limnoriid respiratory protein	688:720	the limnoriid respiratory protein	688:720	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	2	15	theme	few	308:310	arg1	animals					312:318	the few animals	304:318	the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota	304:418	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	4	16	theme	wood	805:808	arg1	incubation					791:800	incubation	791:800	incubation of wood with hemocyanin	791:824	We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin.
30510200	0	17	theme	lignocellulose	23:36	arg1	digestion					38:46	lignocellulose digestion	23:46	lignocellulose digestion	23:46	Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans.
30510200	1	18	with	composite	220:228	arg1	lignin					235:240	lignin	235:240	lignin	235:240	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	5	19	theme	symbionts	1032:1040	arg1	absence					1017:1023	the absence	1013:1023	the absence of gut symbionts	1013:1040	We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts.
30510200	5	20	theme	hemocyanins	939:949	arg1	activity					927:934	this activity	922:934	this activity of hemocyanins	922:949	We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts.
30510200	1	21	theme	insoluble	204:212	arg1	composite					220:228	an insoluble fiber composite	201:228	an insoluble fiber composite with lignin	201:240	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	6	22	theme	lignocellulose	1096:1109	arg1	biorefining					1111:1121	lignocellulose biorefining	1096:1121	lignocellulose biorefining	1096:1121	These findings may hold potential for innovations in lignocellulose biorefining.
30510200	1	23	theme	fiber	214:218	arg1	composite					220:228	an insoluble fiber composite	201:228	an insoluble fiber composite with lignin	201:240	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	2	24	theme	Marine	243:248	arg1	woodborers					261:270	Marine crustacean woodborers	243:270	Marine crustacean woodborers of the genus Limnoria	243:292	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	2	25	theme	resident	400:407	arg1	microbiota					409:418	gut resident microbiota	396:418	gut resident microbiota	396:418	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	3	26	theme	digestive	611:619	arg1	system					621:626	their digestive system	605:626	their digestive system	605:626	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	2	27	theme	gut	396:398	arg1	microbiota					409:418	gut resident microbiota	396:418	gut resident microbiota	396:418	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	3	28	theme	cellulases	591:600	arg1	abundance					578:586	the abundance	574:586	the abundance of cellulases in their digestive system	574:626	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	6	29	from	innovations	1081:1091	arg1	biorefining					1111:1121	lignocellulose biorefining	1096:1121	lignocellulose biorefining	1096:1121	These findings may hold potential for innovations in lignocellulose biorefining.
30510200	0	30	theme	marine	63:68	arg1	crustaceans					70:80	marine crustaceans	63:80	marine crustaceans	63:80	Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans.
30510200	1	31	theme	abundant	127:134	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	31	theme	abundant	127:134	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	32	theme	renewable	136:144	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	32	theme	renewable	136:144	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	2	33	theme	genus	279:283	arg1	Limnoria					285:292	the genus Limnoria	275:292	the genus Limnoria	275:292	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	3	34	from	abundance	578:586	arg1	system					621:626	their digestive system	605:626	their digestive system	605:626	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	3	35	theme	hexose-containing	478:494	arg1	glucomannans					540:551	glucomannans	540:551	glucomannans	540:551	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	3	35	theme	hexose-containing	478:494	arg1	polysaccharides					496:510	hexose-containing polysaccharides	478:510	hexose-containing polysaccharides (mainly cellulose, and also glucomannans)	478:552	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	3	35	theme	hexose-containing	478:494	arg1	cellulose					520:528	cellulose	520:528	cellulose	520:528	Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed.
30510200	5	36	theme	gut	1028:1030	arg1	symbionts					1032:1040	gut symbionts	1028:1040	gut symbionts	1028:1040	We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts.
30510200	5	37	theme	Limnoria	985:992	arg1	ability					974:980	the ability	970:980	the ability of Limnoria to feed on wood in the absence of gut symbionts	970:1040	We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts.
30510200	2	38	theme	recalcitrant	355:366	arg1	material					368:375	this recalcitrant material	350:375	this recalcitrant material	350:375	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	2	39	theme	crustacean	250:259	arg1	woodborers					261:270	Marine crustacean woodborers	243:270	Marine crustacean woodborers of the genus Limnoria	243:292	Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota.
30510200	1	40	theme	rich	157:160	arg1	feedstock					146:154	an abundant renewable feedstock	124:154	an abundant renewable feedstock	124:154	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
30510200	1	40	theme	rich	157:160	arg1	biomass					113:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass	83:119	Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin.
31624921	0	0	theme	guar	80:83	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	1	from	characterization	11:26	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	5	2	theme	Confocal	650:657	arg1	micrographs					659:669	Confocal micrographs	650:669	Confocal micrographs	650:669	Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network.
31624921	5	3	theme	biopolymeric	736:747	arg1	network					749:755	the biopolymeric network	732:755	the biopolymeric network	732:755	Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network.
31624921	9	4	theme	antibacterial	1271:1283	arg1	potency					1285:1291	good antibacterial potency	1266:1291	good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli	1266:1398	All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli.
31624921	6	5	theme	lowest	791:796	arg1	strain					806:811	lowest lattice strain	791:811	lowest lattice strain	791:811	The highest crystallite size and lowest lattice strain were estimated in the citral-containing film.
31624921	0	6	theme	starch/whey	94:104	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	7	from	Synthesis	0:8	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	7	8	theme	structures	959:968	arg1	proportion					933:942	the proportion	929:942	the proportion of the β-sheet structures of the whey protein isolate within the film matrix	929:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	7	9	theme	FTIR	859:862	arg1	analysis					864:871	FTIR analysis	859:871	FTIR analysis	859:871	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	1	10	theme	guar	258:261	arg1	gum					263:265	guar gum	258:265	guar gum	258:265	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	0	11	theme	gum/sago	85:92	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	1	12	theme	gum	263:265	arg1	films					249:253	antimicrobial, composite films	224:253	films	249:253	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	0	13	from	efficacy	47:54	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	8	14	theme	film	1035:1038	arg1	formulation					1040:1050	the film formulation	1031:1050	the film formulation containing combination of carvacrol and citral	1031:1097	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	2	15	theme	essential	409:417	arg1	carvacrol					433:441	carvacrol	433:441	carvacrol	433:441	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	15	theme	essential	409:417	arg1	citral					444:449	citral	444:449	citral	444:449	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	15	theme	essential	409:417	arg1	combination					461:471	their combination	455:471	their combination	455:471	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	15	theme	essential	409:417	arg1	agents					384:389	The model antibacterial agents	360:389	The model antibacterial agents incorporated	360:402	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	15	theme	essential	409:417	arg1	oils					419:422	essential oils	409:422	essential oils	409:422	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	0	16	theme	protein	106:112	arg1	isolate					114:120	guar gum/sago starch/whey protein isolate	80:120	guar gum/sago starch/whey protein isolate	80:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	1	17	theme	gastroenteritis	343:357	arg1	prophylaxis					314:324	the prophylaxis	310:324	the prophylaxis of the bacterial gastroenteritis	310:357	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	10	18	theme	antimicrobial	1448:1460	arg1	films					1462:1466	the prepared antimicrobial films	1435:1466	the prepared antimicrobial films	1435:1466	In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.
31624921	6	19	theme	highest	762:768	arg1	size					782:785	The highest crystallite size	758:785	The highest crystallite size	758:785	The highest crystallite size and lowest lattice strain were estimated in the citral-containing film.
31624921	2	20	theme	antibacterial	370:382	arg1	agents					384:389	The model antibacterial agents	360:389	The model antibacterial agents incorporated	360:402	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	20	theme	antibacterial	370:382	arg1	oils					419:422	essential oils	409:422	essential oils	409:422	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	8	21	theme	tensile	1169:1175	arg1	strength					1177:1184	highest tensile strength	1161:1184	highest tensile strength	1161:1184	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	22	theme	transmission	1135:1146	arg1	WVTR					1154:1157	WVTR	1154:1157	WVTR	1154:1157	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	22	theme	transmission	1135:1146	arg1	rate					1148:1151	the lowest water vapor transmission rate	1112:1151	the lowest water vapor transmission rate (WVTR)	1112:1158	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	10	23	theme	prepared	1439:1446	arg1	films					1462:1466	the prepared antimicrobial films	1435:1466	the prepared antimicrobial films	1435:1466	In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.
31624921	5	24	theme	droplets	716:723	arg1	distribution					692:703	the uniform distribution	680:703	the uniform distribution of the oil droplets within the biopolymeric network	680:755	Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network.
31624921	1	25	theme	bacterial	333:341	arg1	gastroenteritis					343:357	the bacterial gastroenteritis	329:357	the bacterial gastroenteritis	329:357	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	2	26	theme	model	364:368	arg1	agents					384:389	The model antibacterial agents	360:389	The model antibacterial agents incorporated	360:402	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	2	26	theme	model	364:368	arg1	oils					419:422	essential oils	409:422	essential oils	409:422	The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination.
31624921	8	27	theme	highest	1161:1167	arg1	strength					1177:1184	highest tensile strength	1161:1184	highest tensile strength	1161:1184	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	9	28	theme	bacterial	1311:1319	arg1	gastroenteritis					1321:1335	the model bacterial gastroenteritis	1301:1335	the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli	1301:1398	All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli.
31624921	7	29	theme	citral	912:917	arg1	incorporation					895:907	the incorporation	891:907	the incorporation of citral	891:917	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	10	30	theme	gastroenteritis	1519:1533	arg1	prophylaxis					1490:1500	the prophylaxis	1486:1500	the prophylaxis of the bacterial gastroenteritis	1486:1533	In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.
31624921	1	31	theme	sago	268:271	arg1	starch					273:278	sago starch	268:278	sago starch	268:278	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	6	32	theme	crystallite	770:780	arg1	size					782:785	The highest crystallite size	758:785	The highest crystallite size	758:785	The highest crystallite size and lowest lattice strain were estimated in the citral-containing film.
31624921	7	33	theme	β-sheet	951:957	arg1	structures					959:968	the β-sheet structures	947:968	the β-sheet structures of the whey protein isolate within the film matrix	947:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	8	34	theme	vapor	1129:1133	arg1	WVTR					1154:1157	WVTR	1154:1157	WVTR	1154:1157	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	34	theme	vapor	1129:1133	arg1	rate					1148:1151	the lowest water vapor transmission rate	1112:1151	the lowest water vapor transmission rate (WVTR)	1112:1158	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	1	35	theme	starch	273:278	arg1	films					249:253	antimicrobial, composite films	224:253	films	249:253	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	1	36	theme	research	198:205	arg1	aim					186:188	The aim	182:188	The aim of this research	182:205	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	0	37	theme	antimicrobial	33:45	arg1	efficacy					47:54	antimicrobial efficacy	33:54	antimicrobial efficacy	33:54	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	6	38	theme	citral-containing	835:851	arg1	film					853:856	the citral-containing film	831:856	the citral-containing film	831:856	The highest crystallite size and lowest lattice strain were estimated in the citral-containing film.
31624921	9	39	theme	good	1266:1269	arg1	potency					1285:1291	good antibacterial potency	1266:1291	good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli	1266:1398	All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli.
31624921	8	40	theme	Young	1187:1191	arg1	modulus					1195:1201	Young's modulus	1187:1201	Young's modulus	1187:1201	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	5	41	theme	uniform	684:690	arg1	distribution					692:703	the uniform distribution	680:703	the uniform distribution of the oil droplets within the biopolymeric network	680:755	Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network.
31624921	5	42	theme	oil	712:714	arg1	droplets					716:723	the oil droplets	708:723	the oil droplets	708:723	Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network.
31624921	10	43	used	used	1477:1480	arg2	films					1462:1466	the prepared antimicrobial films	1435:1466	the prepared antimicrobial films	1435:1466	In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.
31624921	1	44	theme	whey	285:288	arg1	protein					290:296	whey protein	285:296	whey protein	285:296	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	8	45	theme	water	1123:1127	arg1	WVTR					1154:1157	WVTR	1154:1157	WVTR	1154:1157	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	45	theme	water	1123:1127	arg1	rate					1148:1151	the lowest water vapor transmission rate	1112:1151	the lowest water vapor transmission rate (WVTR)	1112:1158	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	3	46	theme	oils	549:552	arg1	entrapment					531:540	the entrapment	527:540	the entrapment of the oils	527:552	The films became darker and brownish in color due to the entrapment of the oils.
31624921	7	47	theme	film	1009:1012	arg1	matrix					1014:1019	the film matrix	1005:1019	the film matrix	1005:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	8	48	theme	carvacrol	1078:1086	arg1	combination					1063:1073	combination	1063:1073	combination of carvacrol and citral	1063:1097	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	1	49	theme	protein	290:296	arg1	films					249:253	antimicrobial, composite films	224:253	films	249:253	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	8	50	theme	citral	1092:1097	arg1	combination					1063:1073	combination	1063:1073	combination of carvacrol and citral	1063:1097	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	0	51	from	isolate	114:120	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	51	from	isolate	114:120	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	51	from	isolate	114:120	arg1	efficacy					47:54	antimicrobial efficacy	33:54	antimicrobial efficacy	33:54	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	51	from	isolate	114:120	arg1	films					69:73	composite films	59:73	composite films from guar gum/sago starch/whey protein isolate	59:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	7	52	theme	isolate	990:996	arg1	structures					959:968	the β-sheet structures	947:968	the β-sheet structures of the whey protein isolate within the film matrix	947:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	9	53	theme	model	1305:1309	arg1	gastroenteritis					1321:1335	the model bacterial gastroenteritis	1301:1335	the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli	1301:1398	All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli.
31624921	8	54	theme	lowest	1116:1121	arg1	WVTR					1154:1157	WVTR	1154:1157	WVTR	1154:1157	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	54	theme	lowest	1116:1121	arg1	rate					1148:1151	the lowest water vapor transmission rate	1112:1151	the lowest water vapor transmission rate (WVTR)	1112:1158	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	1	55	dep	gum	263:265	arg1	isolate					298:304	isolate	298:304	isolate	298:304	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	0	56	theme	carvacrol-citral	156:171	arg1	mixture					173:179	carvacrol-citral mixture	156:179	carvacrol-citral mixture	156:179	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	9	57	theme	oil-containing	1232:1245	arg1	films					1247:1251	All the oil-containing films	1224:1251	All the oil-containing films	1224:1251	All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli.
31624921	7	58	theme	whey	977:980	arg1	isolate					990:996	the whey protein isolate	973:996	the whey protein isolate within the film matrix	973:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	1	59	theme	antimicrobial	224:236	arg1	films					249:253	antimicrobial, composite films	224:253	films	249:253	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
31624921	0	60	theme	films	69:73	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	60	theme	films	69:73	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	0	60	theme	films	69:73	arg1	efficacy					47:54	antimicrobial efficacy	33:54	antimicrobial efficacy	33:54	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	6	61	theme	lattice	798:804	arg1	strain					806:811	lowest lattice strain	791:811	lowest lattice strain	791:811	The highest crystallite size and lowest lattice strain were estimated in the citral-containing film.
31624921	7	62	theme	protein	982:988	arg1	isolate					990:996	the whey protein isolate	973:996	the whey protein isolate within the film matrix	973:1019	FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix.
31624921	10	63	theme	bacterial	1509:1517	arg1	gastroenteritis					1519:1533	the bacterial gastroenteritis	1505:1533	the bacterial gastroenteritis	1505:1533	In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.
31624921	8	64	contain	containing	1052:1061	arg2	combination					1063:1073	combination	1063:1073	combination of carvacrol and citral	1063:1097	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	8	64	contain	containing	1052:1061	arg1	formulation					1040:1050	the film formulation	1031:1050	the film formulation containing combination of carvacrol and citral	1031:1097	However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young's modulus and work to failure.
31624921	4	65	theme	films	588:592	arg1	rough					603:607	rough	603:607	rough	603:607	The surface of the oil-entrapped films was more rough and coarse compared to the control film.
31624921	4	65	theme	films	588:592	arg1	surface					559:565	The surface	555:565	The surface of the oil-entrapped films	555:592	The surface of the oil-entrapped films was more rough and coarse compared to the control film.
31624921	0	66	theme	composite	59:67	arg1	films					69:73	composite films	59:73	composite films from guar gum/sago starch/whey protein isolate	59:120	Synthesis, characterization, and antimicrobial efficacy of composite films from guar gum/sago starch/whey protein isolate loaded with carvacrol, citral and carvacrol-citral mixture.
31624921	4	67	theme	oil-entrapped	574:586	arg1	films					588:592	the oil-entrapped films	570:592	the oil-entrapped films	570:592	The surface of the oil-entrapped films was more rough and coarse compared to the control film.
31624921	4	68	theme	control	636:642	arg1	film					644:647	the control film	632:647	the control film	632:647	The surface of the oil-entrapped films was more rough and coarse compared to the control film.
31624921	1	69	theme	composite	239:247	arg1	films					249:253	antimicrobial, composite films	224:253	films	249:253	The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis.
30733471	3	0	theme	spore-forming	141:153	arg1	bacterium					181:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	0	theme	spore-forming	141:153	arg1	WSUCF1					114:119	WSUCF1	114:119	WSUCF1	114:119	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	10	1	theme	species	1301:1307	arg1	analysis					1262:1269	genome analysis	1255:1269	genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1255:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	8	2	theme	quorum	890:895	arg1	lactonase					907:915	quorum quenching lactonase	890:915	quorum quenching lactonase	890:915	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	7	3	theme	sophisticated	761:773	arg1	systems					775:781	sophisticated systems	761:781	sophisticated systems for its adaptation under thermophilic conditions	761:830	Additionally, the genome description of strain WSUCF1 suggests sophisticated systems for its adaptation under thermophilic conditions.
30733471	6	4	theme	EPS	648:650	arg1	pathway					616:622	the biosynthesis pathway	599:622	the biosynthesis pathway	599:622	Both the biosynthesis pathway and export mechanism of EPS were proposed based on functional annotation.
30733471	6	4	theme	EPS	648:650	arg1	mechanism					635:643	export mechanism	628:643	export mechanism of EPS	628:650	Both the biosynthesis pathway and export mechanism of EPS were proposed based on functional annotation.
30733471	5	5	theme	WSUCF1	380:385	arg1	analysis					368:375	The whole-genome analysis	351:375	The whole-genome analysis of WSUCF1	351:385	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	8	6	theme	various	1006:1012	arg1	applications					1014:1025	various applications	1006:1025	various applications in biotechnology and biomedicine	1006:1058	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	9	7	contain	has	1108:1110	arg2	producer					1142:1149	a thermophilic EPS producer	1123:1149	a thermophilic EPS producer for a broad range of industrial applications	1123:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	9	7	contain	has	1108:1110	arg2	promise					1112:1118	promise	1112:1118	promise	1112:1118	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	9	7	contain	has	1108:1110	arg1	WSUCF1					1101:1106	strain WSUCF1	1094:1106	strain WSUCF1	1094:1106	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	5	8	theme	nucleotide	449:458	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	7	9	theme	WSUCF1	745:750	arg1	description					723:733	the genome description	712:733	the genome description of strain WSUCF1	712:750	Additionally, the genome description of strain WSUCF1 suggests sophisticated systems for its adaptation under thermophilic conditions.
30733471	9	10	theme	strain	1094:1099	arg1	WSUCF1					1101:1106	strain WSUCF1	1094:1106	strain WSUCF1	1094:1106	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	10	11	theme	EPS	1433:1435	arg1	production					1437:1446	tailor-made EPS production	1421:1446	tailor-made EPS production by thermophilic bacteria	1421:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	5	12	theme	monosaccharide	502:515	arg1	units					517:521	monosaccharide units	502:521	monosaccharide units	502:521	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	8	13	theme	genes	849:853	arg1	presence					837:844	The presence	833:844	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance	833:960	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	9	14	theme	industrial	1172:1181	arg1	applications					1183:1194	industrial applications	1172:1194	industrial applications	1172:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	3	15	theme	thermophilic	168:179	arg1	bacterium					181:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	15	theme	thermophilic	168:179	arg1	WSUCF1					114:119	WSUCF1	114:119	WSUCF1	114:119	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	8	16	theme	polyketide	918:927	arg1	synthesis					929:937	polyketide synthesis	918:937	polyketide synthesis	918:937	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	6	17	theme	biosynthesis	603:614	arg1	pathway					616:622	the biosynthesis pathway	599:622	the biosynthesis pathway	599:622	Both the biosynthesis pathway and export mechanism of EPS were proposed based on functional annotation.
30733471	6	18	theme	export	628:633	arg1	mechanism					635:643	export mechanism	628:643	export mechanism of EPS	628:650	Both the biosynthesis pathway and export mechanism of EPS were proposed based on functional annotation.
30733471	8	19	theme	arsenic	943:949	arg1	resistance					951:960	arsenic resistance	943:960	arsenic resistance	943:960	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	7	20	theme	thermophilic	808:819	arg1	conditions					821:830	thermophilic conditions	808:830	thermophilic conditions	808:830	Additionally, the genome description of strain WSUCF1 suggests sophisticated systems for its adaptation under thermophilic conditions.
30733471	3	21	theme	compost	236:242	arg1	facility					244:251	a compost facility	234:251	a compost facility	234:251	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	22	theme	soil	208:211	arg1	sample					213:218	a soil sample	206:218	a soil sample obtained from a compost facility	206:251	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	4	23	theme	different	312:320	arg1	source					343:348	the carbon source	332:348	the carbon source	332:348	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	4	23	theme	different	312:320	arg1	sugars					322:327	different sugars	312:327	different sugars	312:327	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	0	24	theme	Genome	0:5	arg1	analysis					7:14	Genome analysis	0:14	Genome analysis of a thermophilic exopolysaccharide-producing bacterium	0:70	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	9	25	theme	applications	1183:1194	arg1	range					1163:1167	a broad range	1155:1167	a broad range of industrial applications	1155:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	3	26	attach	isolated	192:199	arg2	WSUCF1					114:119	WSUCF1	114:119	WSUCF1	114:119	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	26	attach	isolated	192:199	arg1	sample					213:218	a soil sample	206:218	a soil sample obtained from a compost facility	206:251	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	26	attach	isolated	192:199	arg2	bacterium					181:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	10	27	theme	thermophilic	1276:1287	arg1	species					1301:1307	a thermophilic Geobacillus species	1274:1307	a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1274:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	10	28	theme	tailor-made	1421:1431	arg1	production					1437:1446	tailor-made EPS production	1421:1446	tailor-made EPS production by thermophilic bacteria	1421:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	0	29	dep	sp	86:87	arg1	analysis					7:14	Genome analysis	0:14	Genome analysis of a thermophilic exopolysaccharide-producing bacterium	0:70	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	9	30	theme	present	1065:1071	arg1	study					1073:1077	The present study	1061:1077	The present study	1061:1077	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	9	31	theme	broad	1157:1161	arg1	range					1163:1167	a broad range	1155:1167	a broad range of industrial applications	1155:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	5	32	theme	chain	553:557	arg1	export					524:529	export	524:529	export of the polysaccharide chain	524:557	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	32	theme	chain	553:557	arg1	assembly					490:497	assembly	490:497	assembly of monosaccharide units	490:521	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	32	theme	chain	553:557	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	32	theme	chain	553:557	arg1	regulation					564:573	regulation	564:573	regulation of EPS production	564:591	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	33	theme	whole-genome	355:366	arg1	analysis					368:375	The whole-genome analysis	351:375	The whole-genome analysis of WSUCF1	351:385	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	34	theme	EPS	578:580	arg1	production					582:591	EPS production	578:591	EPS production	578:591	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	35	theme	units	517:521	arg1	export					524:529	export	524:529	export of the polysaccharide chain	524:557	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	35	theme	units	517:521	arg1	assembly					490:497	assembly	490:497	assembly of monosaccharide units	490:521	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	35	theme	units	517:521	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	35	theme	units	517:521	arg1	regulation					564:573	regulation	564:573	regulation of EPS production	564:591	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	4	36	theme	producing	285:293	arg1	capability					295:304	EPS producing capability	281:304	EPS producing capability	281:304	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	0	37	theme	exopolysaccharide-producing	34:60	arg1	bacterium					62:70	a thermophilic exopolysaccharide-producing bacterium	19:70	a thermophilic exopolysaccharide-producing bacterium	19:70	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	7	38	theme	strain	738:743	arg1	WSUCF1					745:750	strain WSUCF1	738:750	strain WSUCF1	738:750	Additionally, the genome description of strain WSUCF1 suggests sophisticated systems for its adaptation under thermophilic conditions.
30733471	9	39	theme	thermophilic	1125:1136	arg1	producer					1142:1149	a thermophilic EPS producer	1123:1149	a thermophilic EPS producer for a broad range of industrial applications	1123:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	9	39	theme	thermophilic	1125:1136	arg1	promise					1112:1118	promise	1112:1118	promise	1112:1118	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	4	40	theme	EPS	281:283	arg1	capability					295:304	EPS producing capability	281:304	EPS producing capability	281:304	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	8	41	from	applications	1014:1025	arg1	biomedicine					1048:1058	biomedicine	1048:1058	biomedicine	1048:1058	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	8	41	from	applications	1014:1025	arg1	biotechnology					1030:1042	biotechnology	1030:1042	biotechnology	1030:1042	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	0	42	theme	thermophilic	21:32	arg1	bacterium					62:70	a thermophilic exopolysaccharide-producing bacterium	19:70	a thermophilic exopolysaccharide-producing bacterium	19:70	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	10	43	theme	genome	1255:1260	arg1	analysis					1262:1269	genome analysis	1255:1269	genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1255:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	7	44	theme	genome	716:721	arg1	description					723:733	the genome description	712:733	the genome description of strain WSUCF1	712:750	Additionally, the genome description of strain WSUCF1 suggests sophisticated systems for its adaptation under thermophilic conditions.
30733471	10	45	theme	Geobacillus	1289:1299	arg1	species					1301:1307	a thermophilic Geobacillus species	1274:1307	a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1274:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	8	46	theme	potential	982:990	arg1	candidate					992:1000	a potential candidate	980:1000	a potential candidate for various applications in biotechnology and biomedicine	980:1058	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	3	47	theme	Gram-positive	126:138	arg1	bacterium					181:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	47	theme	Gram-positive	126:138	arg1	WSUCF1					114:119	WSUCF1	114:119	WSUCF1	114:119	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	0	48	theme	bacterium	62:70	arg1	analysis					7:14	Genome analysis	0:14	Genome analysis of a thermophilic exopolysaccharide-producing bacterium	0:70	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	2	49	theme	Geobacillus	98:108	arg1	sp					110:111	Geobacillus sp	98:111	Geobacillus sp.	98:112	Geobacillus sp.
30733471	10	50	from	report	1245:1250	arg1	analysis					1262:1269	genome analysis	1255:1269	genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1255:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	4	51	theme	carbon	336:341	arg1	source					343:348	the carbon source	332:348	the carbon source	332:348	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	4	51	theme	carbon	336:341	arg1	sugars					322:327	different sugars	312:327	different sugars	312:327	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	8	52	theme	CRISPR-Cas	871:880	arg1	system					882:887	CRISPR-Cas system	871:887	CRISPR-Cas system	871:887	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	5	53	theme	sugar	460:464	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	10	54	theme	thermophilic	1451:1462	arg1	bacteria					1464:1471	thermophilic bacteria	1451:1471	thermophilic bacteria	1451:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	5	55	theme	production	582:591	arg1	export					524:529	export	524:529	export of the polysaccharide chain	524:557	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	55	theme	production	582:591	arg1	assembly					490:497	assembly	490:497	assembly of monosaccharide units	490:521	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	55	theme	production	582:591	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	55	theme	production	582:591	arg1	regulation					564:573	regulation	564:573	regulation of EPS production	564:591	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	5	56	theme	precursor	466:474	arg1	biosynthesis					476:487	nucleotide sugar precursor biosynthesis	449:487	nucleotide sugar precursor biosynthesis	449:487	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	10	57	theme	first	1239:1243	arg1	this					1227:1230	this	1227:1230	this	1227:1230	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	10	57	theme	first	1239:1243	arg1	report					1245:1250	the first report	1235:1250	the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria	1235:1471	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	10	58	theme	EPS	1325:1327	arg1	biosynthesis					1329:1340	its EPS biosynthesis	1321:1340	its EPS biosynthesis	1321:1340	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	5	59	theme	polysaccharide	538:551	arg1	chain					553:557	the polysaccharide chain	534:557	the polysaccharide chain	534:557	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
30733471	4	60	theme	Strain	254:259	arg1	WSUCF1					261:266	Strain WSUCF1	254:266	Strain WSUCF1	254:266	Strain WSUCF1 demonstrated EPS producing capability using different sugars as the carbon source.
30733471	9	61	theme	EPS	1138:1140	arg1	producer					1142:1149	a thermophilic EPS producer	1123:1149	a thermophilic EPS producer for a broad range of industrial applications	1123:1194	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	9	61	theme	EPS	1138:1140	arg1	promise					1112:1118	promise	1112:1118	promise	1112:1118	The present study indicates that strain WSUCF1 has promise as a thermophilic EPS producer for a broad range of industrial applications.
30733471	3	62	theme	aerobic	156:162	arg1	bacterium					181:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	a Gram-positive, spore-forming, aerobic and thermophilic bacterium	124:189	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	3	62	theme	aerobic	156:162	arg1	WSUCF1					114:119	WSUCF1	114:119	WSUCF1	114:119	WSUCF1 is a Gram-positive, spore-forming, aerobic and thermophilic bacterium, isolated from a soil sample obtained from a compost facility.
30733471	6	63	theme	functional	675:684	arg1	annotation					686:695	functional annotation	675:695	functional annotation	675:695	Both the biosynthesis pathway and export mechanism of EPS were proposed based on functional annotation.
30733471	0	64	theme	Geobacillus	74:84	arg1	sp					86:87	Geobacillus sp	74:87	Geobacillus sp	74:87	Genome analysis of a thermophilic exopolysaccharide-producing bacterium - Geobacillus sp.
30733471	10	65	theme	enhanced	1402:1409	arg1	yield					1411:1415	enhanced yield	1402:1415	enhanced yield	1402:1415	To the best of our knowledge, this is the first report on genome analysis of a thermophilic Geobacillus species focusing on its EPS biosynthesis and transportation, which will likely pave the way for both enhanced yield and tailor-made EPS production by thermophilic bacteria.
30733471	8	66	theme	quenching	897:905	arg1	lactonase					907:915	quorum quenching lactonase	890:915	quorum quenching lactonase	890:915	The presence of genes associated with CRISPR-Cas system, quorum quenching lactonase, polyketide synthesis and arsenic resistance makes this strain a potential candidate for various applications in biotechnology and biomedicine.
30733471	5	67	theme	essential	417:425	arg1	genes					427:431	the essential genes	413:431	the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production	413:591	The whole-genome analysis of WSUCF1 was performed to disclose the essential genes correlated with nucleotide sugar precursor biosynthesis, assembly of monosaccharide units, export of the polysaccharide chain, and regulation of EPS production.
31012794	4	0	theme	FOS	665:667	arg1	yield					669:673	the maximum FOS yield	653:673	the maximum FOS yield (71.81%)	653:682	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	4	0	theme	FOS	665:667	arg1	%					681:681	71.81%	676:681	71.81%	676:681	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	0	1	theme	fructotransferase	84:100	arg1	activity					102:109	fructotransferase activity	84:109	fructotransferase activity	84:109	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	4	2	theme	FOS	787:789	arg1	yield					791:795	the FOS yield	783:795	the FOS yield (57.13%) using Pectinex Ultra SP-L	783:830	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	4	2	theme	FOS	787:789	arg1	%					803:803	57.13%	798:803	57.13%	798:803	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	4	3	theme	maximum	657:663	arg1	yield					669:673	the maximum FOS yield	653:673	the maximum FOS yield (71.81%)	653:682	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	4	3	theme	maximum	657:663	arg1	%					681:681	71.81%	676:681	71.81%	676:681	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	7	4	theme	FOS	1406:1408	arg1	production					1410:1419	FOS production	1406:1419	FOS production	1406:1419	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	0	5	theme	Pectinex	117:124	arg1	SP-L					132:135	Pectinex Ultra SP-L	117:135	Pectinex Ultra SP-L	117:135	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	0	6	from	Effects	0:6	arg1	activity					102:109	fructotransferase activity	84:109	fructotransferase activity	84:109	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	0	6	from	Effects	0:6	arg1	synthesis					44:52	effective synthesis	34:52	effective synthesis of fructooligosaccharides	34:78	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	7	7	theme	Ultra	1266:1270	arg1	SP-L					1272:1275	Pectinex Ultra SP-L	1257:1275	Pectinex Ultra SP-L	1257:1275	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	5	8	theme	only	1114:1117	arg1	GF2					1119:1121	only GF2	1114:1121	only GF2	1114:1121	The FOS composition produced by Pectinex Ultra SP-L under the UHP was 1-kestose (GF2), nystose (GF3), and 1F-fructofuranosylnystose (GF4), whereas the FOS produced by inulinase composed of only GF2 and GF3.
31012794	1	9	theme	enzymatic	257:265	arg1	synthesis					267:275	the enzymatic synthesis	253:275	the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase	253:350	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	7	10	theme	inulinase	1293:1301	arg1	level					1312:1316	inulinase Pressure level	1293:1316	inulinase Pressure level	1293:1316	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	7	11	theme	SP-L	1272:1275	arg1	activity					1245:1252	the activity	1241:1252	the activity of Pectinex Ultra SP-L	1241:1275	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	4	12	with	20 min	722:727	arg1	300 g/L					734:740	300 g/L	734:740	300 g/L of initial sucrose as a substrate	734:774	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	6	13	theme	industrial	1173:1182	arg1	application					1184:1194	the industrial application	1169:1194	the industrial application	1169:1194	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	7	14	theme	Pressure	1303:1310	arg1	level					1312:1316	inulinase Pressure level	1293:1316	inulinase Pressure level	1293:1316	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	1	15	theme	various	190:196	arg1	levels					198:203	various levels	190:203	various levels of ultra-high pressure (UHP)	190:232	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	0	16	theme	Ultra	126:130	arg1	SP-L					132:135	Pectinex Ultra SP-L	117:135	Pectinex Ultra SP-L	117:135	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	4	17	theme	initial	893:899	arg1	sucrose					901:907	initial sucrose	893:907	initial sucrose as a substrate	893:922	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	7	18	theme	Pectinex	1257:1264	arg1	SP-L					1272:1275	Pectinex Ultra SP-L	1257:1275	Pectinex Ultra SP-L	1257:1275	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	6	19	theme	FOS	1200:1202	arg1	production					1204:1213	FOS production	1200:1213	FOS production	1200:1213	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	3	20	theme	pressure	540:547	arg1	times					563:567	the reaction times	550:567	the reaction times	550:567	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	3	20	theme	pressure	540:547	arg1	concentrations					594:607	the initial sucrose concentrations	574:607	the initial sucrose concentrations	574:607	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	3	20	theme	pressure	540:547	arg1	levels					530:535	the levels	526:535	the levels of pressure	526:547	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	2	21	theme	FOS	382:384	arg1	yields					386:391	the FOS yields	378:391	the FOS yields up to 2.5- and 1.5-fold, respectively	378:429	The combination enhanced the FOS yields up to 2.5- and 1.5-fold, respectively, compared to atmospheric condition (0.1 MPa).
31012794	6	22	theme	useful	1154:1159	arg1	UHP					1145:1147	The combined UHP	1132:1147	The combined UHP	1132:1147	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	6	22	theme	useful	1154:1159	arg1	tool					1161:1164	a useful tool	1152:1164	a useful tool in the industrial application for FOS production	1152:1213	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	5	23	theme	Ultra	966:970	arg1	SP-L					972:975	Pectinex Ultra SP-L	957:975	Pectinex Ultra SP-L	957:975	The FOS composition produced by Pectinex Ultra SP-L under the UHP was 1-kestose (GF2), nystose (GF3), and 1F-fructofuranosylnystose (GF4), whereas the FOS produced by inulinase composed of only GF2 and GF3.
31012794	0	24	theme	pressure	22:29	arg1	Effects					0:6	Effects	0:6	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.	0:173	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	4	25	theme	sucrose	753:759	arg1	300 g/L					734:740	300 g/L	734:740	300 g/L of initial sucrose as a substrate	734:774	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	0	26	theme	ultra-high	11:20	arg1	pressure					22:29	ultra-high pressure	11:29	ultra-high pressure	11:29	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	1	27	theme	fructooligosaccharide	284:304	arg1	synthesis					267:275	the enzymatic synthesis	253:275	the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase	253:350	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	4	28	theme	Ultra	821:825	arg1	SP-L					827:830	Pectinex Ultra SP-L	812:830	Pectinex Ultra SP-L	812:830	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	5	29	theme	Pectinex	957:964	arg1	SP-L					972:975	Pectinex Ultra SP-L	957:975	Pectinex Ultra SP-L	957:975	The FOS composition produced by Pectinex Ultra SP-L under the UHP was 1-kestose (GF2), nystose (GF3), and 1F-fructofuranosylnystose (GF4), whereas the FOS produced by inulinase composed of only GF2 and GF3.
31012794	1	30	theme	ultra-high	208:217	arg1	UHP					229:231	UHP	229:231	UHP	229:231	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	1	30	theme	ultra-high	208:217	arg1	pressure					219:226	ultra-high pressure	208:226	ultra-high pressure (UHP)	208:232	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	0	31	theme	effective	34:42	arg1	synthesis					44:52	effective synthesis	34:52	effective synthesis of fructooligosaccharides	34:78	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	6	32	from	tool	1161:1164	arg1	application					1184:1194	the industrial application	1169:1194	the industrial application	1169:1194	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	0	33	theme	Aspergillus	156:166	arg1	niger					168:172	Aspergillus niger	156:172	Aspergillus niger	156:172	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	4	34	theme	combined	614:621	arg1	UHP					623:625	The combined UHP	610:625	The combined UHP	610:625	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	2	35	dep	2.5-	399:402	arg1	up					393:394	up	393:394	up	393:394	The combination enhanced the FOS yields up to 2.5- and 1.5-fold, respectively, compared to atmospheric condition (0.1 MPa).
31012794	7	36	dep	Highlights	1216:1225	arg1	activated					1231:1239	activated	1231:1239	activated the activity of Pectinex Ultra SP-L	1231:1275	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	7	36	dep	Highlights	1216:1225	arg1	inactivated					1281:1291	inactivated	1281:1291	inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min	1281:1462	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	1	37	theme	pressure	219:226	arg1	levels					198:203	various levels	190:203	various levels of ultra-high pressure (UHP)	190:232	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	4	38	theme	initial	745:751	arg1	sucrose					753:759	initial sucrose	745:759	initial sucrose	745:759	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	2	39	theme	atmospheric	444:454	arg1	0.1 MPa					467:473	0.1 MPa	467:473	0.1 MPa	467:473	The combination enhanced the FOS yields up to 2.5- and 1.5-fold, respectively, compared to atmospheric condition (0.1 MPa).
31012794	2	39	theme	atmospheric	444:454	arg1	condition					456:464	atmospheric condition	444:464	atmospheric condition (0.1 MPa)	444:474	The combination enhanced the FOS yields up to 2.5- and 1.5-fold, respectively, compared to atmospheric condition (0.1 MPa).
31012794	7	40	dep	inactivated	1281:1291	arg1	affect					1365:1370	affect	1365:1370	inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min	1281:1462	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	7	41	theme	FOS	1372:1374	arg1	yields					1376:1381	FOS yields	1372:1381	FOS yields	1372:1381	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	6	42	theme	combined	1136:1143	arg1	UHP					1145:1147	The combined UHP	1132:1147	The combined UHP	1132:1147	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	6	42	theme	combined	1136:1143	arg1	tool					1161:1164	a useful tool	1152:1164	a useful tool in the industrial application for FOS production	1152:1213	The combined UHP is a useful tool in the industrial application for FOS production.
31012794	7	43	theme	UHP	1389:1391	arg1	UHP					1393:1395	UHP UHP	1389:1395	UHP UHP	1389:1395	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	4	44	theme	sucrose	901:907	arg1	600 g/L					882:888	600 g/L	882:888	600 g/L of initial sucrose as a substrate	882:922	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	5	45	theme	FOS	929:931	arg1	1-kestose					995:1003	1-kestose	995:1003	1-kestose (GF2)	995:1009	The FOS composition produced by Pectinex Ultra SP-L under the UHP was 1-kestose (GF2), nystose (GF3), and 1F-fructofuranosylnystose (GF4), whereas the FOS produced by inulinase composed of only GF2 and GF3.
31012794	5	45	theme	FOS	929:931	arg1	composition					933:943	The FOS composition	925:943	The FOS composition produced by Pectinex Ultra SP-L under the UHP	925:989	The FOS composition produced by Pectinex Ultra SP-L under the UHP was 1-kestose (GF2), nystose (GF3), and 1F-fructofuranosylnystose (GF4), whereas the FOS produced by inulinase composed of only GF2 and GF3.
31012794	3	46	theme	reaction	554:561	arg1	times					563:567	the reaction times	550:567	the reaction times	550:567	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	4	47	with	15 min	870:875	arg1	600 g/L					882:888	600 g/L	882:888	600 g/L of initial sucrose as a substrate	882:922	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	1	48	theme	Pectinex	318:325	arg1	SP-L					333:336	Pectinex Ultra SP-L	318:336	Pectinex Ultra SP-L	318:336	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
31012794	4	49	theme	Pectinex	812:819	arg1	SP-L					827:830	Pectinex Ultra SP-L	812:830	Pectinex Ultra SP-L	812:830	The combined UHP and inulinase showed that the maximum FOS yield (71.81%) was obtained under UHP at 200 MPa for 20 min with 300 g/L of initial sucrose as a substrate, while the FOS yield (57.13%) using Pectinex Ultra SP-L was obtained under UHP at 300 MPa for 15 min with 600 g/L of initial sucrose as a substrate.
31012794	3	50	theme	initial	578:584	arg1	concentrations					594:607	the initial sucrose concentrations	574:607	the initial sucrose concentrations	574:607	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	7	51	theme	time-saving	1426:1436	arg1	benefits					1438:1445	time-saving benefits	1426:1445	time-saving benefits within 15-20 min	1426:1462	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	3	52	theme	sucrose	586:592	arg1	concentrations					594:607	the initial sucrose concentrations	574:607	the initial sucrose concentrations	574:607	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	7	53	theme	sucrose	1329:1335	arg1	concentration					1337:1349	sucrose concentration	1329:1349	sucrose concentration	1329:1349	Highlights UHP activated the activity of Pectinex Ultra SP-L yet inactivated inulinase Pressure level, time, and sucrose concentration significantly affect FOS yields under UHP UHP enhanced FOS production with time-saving benefits within 15-20 min.
31012794	3	54	theme	enzymatic	490:498	arg1	dependent					513:521	dependent	513:521	dependent	513:521	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	3	54	theme	enzymatic	490:498	arg1	reaction					500:507	the enzymatic reaction	486:507	the enzymatic reaction	486:507	However, the enzymatic reaction was dependent on the levels of pressure, the reaction times, and the initial sucrose concentrations.
31012794	0	55	theme	fructooligosaccharides	57:78	arg1	activity					102:109	fructotransferase activity	84:109	fructotransferase activity	84:109	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	0	55	theme	fructooligosaccharides	57:78	arg1	synthesis					44:52	effective synthesis	34:52	effective synthesis of fructooligosaccharides	34:78	Effects of ultra-high pressure on effective synthesis of fructooligosaccharides and fructotransferase activity using Pectinex Ultra SP-L and inulinase from Aspergillus niger.
31012794	1	56	theme	Ultra	327:331	arg1	SP-L					333:336	Pectinex Ultra SP-L	318:336	Pectinex Ultra SP-L	318:336	In this study, various levels of ultra-high pressure (UHP) were combined with the enzymatic synthesis of the fructooligosaccharide (FOS) using Pectinex Ultra SP-L and inulinase.
30703751	7	0	theme	degradable	1434:1443	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	0	theme	degradable	1434:1443	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	1	1	theme	Natural	165:171	arg1	films					188:192	Natural bio-based zein films	165:192	Natural bio-based zein films	165:192	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	5	2	theme	electron	997:1004	arg1	microscopy					1006:1015	transmission electron microscopy	984:1015	transmission electron microscopy (TEM)	984:1021	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	2	theme	electron	997:1004	arg1	TEM					1018:1020	TEM	1018:1020	TEM	1018:1020	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	0	3	theme	essential	87:95	arg1	oil					97:99	cinnamon essential oil	78:99	cinnamon essential oil	78:99	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	7	4	theme	film-forming	1445:1456	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	4	theme	film-forming	1445:1456	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	1	5	theme	bio-based	173:181	arg1	films					188:192	Natural bio-based zein films	165:192	Natural bio-based zein films	165:192	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	6	theme	chitosan	258:265	arg1	CNPs					282:285	CNPs	282:285	CNPs	282:285	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	6	theme	chitosan	258:265	arg1	nanoparticles					267:279	cinnamon essential oil (CEO) and chitosan nanoparticles	225:279	cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs)	225:286	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	0	7	theme	Physical	102:109	arg1	attributes					153:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	6	8	theme	zein	1235:1238	arg1	films					1240:1244	zein films	1235:1244	zein films	1235:1244	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	6	9	theme	CEO	1193:1195	arg1	addition					1181:1188	addition	1181:1188	addition of CEO alone and in combination with CNPs in zein films	1181:1244	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	7	10	theme	reinforced	1369:1378	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	10	theme	reinforced	1369:1378	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	6	11	from	CNPs	1227:1230	arg1	films					1240:1244	zein films	1235:1244	zein films	1235:1244	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	5	12	theme	scanning	900:907	arg1	microscopy					918:927	scanning electron microscopy	900:927	scanning electron microscopy (SEM)	900:933	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	12	theme	scanning	900:907	arg1	SEM					930:932	SEM	930:932	SEM	930:932	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	7	13	theme	zein	1380:1383	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	13	theme	zein	1380:1383	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	4	14	theme	diffraction	680:690	arg1	results					698:704	X-ray diffraction (XRD) results	674:704	X-ray diffraction (XRD) results	674:704	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	2	15	theme	films	483:487	arg1	properties					451:460	The physical, mechanical, structural and antibacterial properties	396:460	The physical, mechanical, structural and antibacterial properties of the enriched zein films	396:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	1	16	theme	zein	183:186	arg1	films					188:192	Natural bio-based zein films	165:192	Natural bio-based zein films	165:192	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	2	17	theme	zein	478:481	arg1	films					483:487	the enriched zein films	465:487	the enriched zein films	465:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	1	18	dep	%	292:292	arg1	amounts					307:313	amounts	307:313	amounts	307:313	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	6	19	theme	antimicrobial	1028:1040	arg1	properties					1042:1051	The antimicrobial properties	1024:1051	The antimicrobial properties	1024:1051	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	0	20	theme	mechanical	112:121	arg1	attributes					153:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	0	21	theme	zein	14:17	arg1	composites					24:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	6	22	theme	microorganisms	1318:1331	arg1	growth					1308:1313	the growth	1304:1313	the growth of microorganisms	1304:1331	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	2	23	theme	enriched	469:476	arg1	films					483:487	the enriched zein films	465:487	the enriched zein films	465:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	4	24	theme	zein	707:710	arg1	film					712:715	zein film	707:715	zein film	707:715	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	0	25	theme	Biodegradable	0:12	arg1	composites					24:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	5	26	theme	transmission	984:995	arg1	microscopy					1006:1015	transmission electron microscopy	984:1015	transmission electron microscopy (TEM)	984:1021	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	26	theme	transmission	984:995	arg1	TEM					1018:1020	TEM	1018:1020	TEM	1018:1020	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	6	27	theme	significant	1282:1292	arg1	effect					1294:1299	no significant effect	1279:1299	no significant effect	1279:1299	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	5	28	theme	zein	872:875	arg1	film					877:880	the zein film	868:880	the zein film	868:880	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	2	29	theme	physical	400:407	arg1	properties					451:460	The physical, mechanical, structural and antibacterial properties	396:460	The physical, mechanical, structural and antibacterial properties of the enriched zein films	396:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	0	30	theme	structural	124:133	arg1	attributes					153:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	0	31	theme	film	19:22	arg1	composites					24:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites	0:33	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	5	32	theme	electron	909:916	arg1	microscopy					918:927	scanning electron microscopy	900:927	scanning electron microscopy (SEM)	900:933	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	32	theme	electron	909:916	arg1	SEM					930:932	SEM	930:932	SEM	930:932	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	4	33	theme	X-ray	674:678	arg1	XRD					693:695	XRD	693:695	XRD	693:695	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	4	33	theme	X-ray	674:678	arg1	diffraction					680:690	X-ray diffraction	674:690	X-ray diffraction (XRD) results	674:704	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	5	34	theme	Nano-scale	806:815	arg1	size					817:820	Nano-scale size	806:820	Nano-scale size of CNPs and their uniform distribution within the zein film	806:880	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	2	35	theme	antibacterial	437:449	arg1	properties					451:460	The physical, mechanical, structural and antibacterial properties	396:460	The physical, mechanical, structural and antibacterial properties of the enriched zein films	396:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	0	36	theme	antimicrobial	139:151	arg1	attributes					153:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Physical, mechanical, structural and antimicrobial attributes	102:162	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	5	37	theme	uniform	840:846	arg1	distribution					848:859	their uniform distribution	834:859	their uniform distribution within the zein film	834:880	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	1	38	theme	cinnamon	225:232	arg1	CEO					249:251	CEO	249:251	CEO	249:251	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	38	theme	cinnamon	225:232	arg1	oil					244:246	cinnamon essential oil	225:246	cinnamon essential oil (CEO)	225:252	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	5	39	theme	CNPs	825:828	arg1	size					817:820	Nano-scale size	806:820	Nano-scale size of CNPs and their uniform distribution within the zein film	806:880	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	7	40	theme	packaging	1477:1485	arg1	applications					1487:1498	food packaging applications	1472:1498	food packaging applications	1472:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	1	41	theme	essential	234:242	arg1	CEO					249:251	CEO	249:251	CEO	249:251	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	41	theme	essential	234:242	arg1	oil					244:246	cinnamon essential oil	225:246	cinnamon essential oil (CEO)	225:252	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	2	42	theme	structural	422:431	arg1	properties					451:460	The physical, mechanical, structural and antibacterial properties	396:460	The physical, mechanical, structural and antibacterial properties of the enriched zein films	396:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	1	43	theme	mechanical	350:359	arg1	functionalities					379:393	mechanical and antimicrobial functionalities	350:393	mechanical and antimicrobial functionalities	350:393	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	4	44	theme	CNPs-CEO	796:803	arg1	combination					781:791	combination	781:791	combination of CNPs-CEO	781:803	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	7	45	theme	based	1385:1389	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	45	theme	based	1385:1389	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	0	46	theme	chitosan	51:58	arg1	nanoparticles					60:72	chitosan nanoparticles	51:72	chitosan nanoparticles	51:72	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	6	47	theme	zein	1265:1268	arg1	film					1270:1273	CNPs-loaded zein film	1253:1273	CNPs-loaded zein film	1253:1273	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	3	48	theme	composite	650:658	arg1	elongation					626:635	the elongation	622:635	the elongation of zein film composite	622:658	The results showed that the combination of CEO-CNPs significantly improves the tensile strength and decreases the elongation of zein film composite.
30703751	3	49	theme	CEO-CNPs	555:562	arg1	combination					540:550	the combination	536:550	the combination of CEO-CNPs	536:562	The results showed that the combination of CEO-CNPs significantly improves the tensile strength and decreases the elongation of zein film composite.
30703751	6	50	contain	had	1275:1277	arg2	effect					1294:1299	no significant effect	1279:1299	no significant effect	1279:1299	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	6	50	contain	had	1275:1277	arg1	film					1270:1273	CNPs-loaded zein film	1253:1273	CNPs-loaded zein film	1253:1273	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	4	51	theme	CNPs	767:770	arg1	presence					755:762	the presence	751:762	the presence of CNPs	751:770	According to X-ray diffraction (XRD) results, zein film experienced more crystallinity in the presence of CNPs and also combination of CNPs-CEO.
30703751	6	52	theme	CNPs-loaded	1253:1263	arg1	film					1270:1273	CNPs-loaded zein film	1253:1273	CNPs-loaded zein film	1253:1273	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30703751	1	53	theme	antimicrobial	365:377	arg1	functionalities					379:393	mechanical and antimicrobial functionalities	350:393	mechanical and antimicrobial functionalities	350:393	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	54	theme	oil	244:246	arg1	CNPs					282:285	CNPs	282:285	CNPs	282:285	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	1	54	theme	oil	244:246	arg1	nanoparticles					267:279	cinnamon essential oil (CEO) and chitosan nanoparticles	225:279	cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs)	225:286	Natural bio-based zein films were prepared by incorporating cinnamon essential oil (CEO) and chitosan nanoparticles (CNPs) at 2% and 4% (w/w) amounts, respectively, in order to provide mechanical and antimicrobial functionalities.
30703751	3	55	theme	zein	640:643	arg1	composite					650:658	zein film composite	640:658	zein film composite	640:658	The results showed that the combination of CEO-CNPs significantly improves the tensile strength and decreases the elongation of zein film composite.
30703751	5	56	theme	dynamic	949:955	arg1	DLS					975:977	DLS	975:977	DLS	975:977	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	56	theme	dynamic	949:955	arg1	scattering					963:972	dynamic light scattering	949:972	dynamic light scattering (DLS)	949:978	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	0	57	theme	cinnamon	78:85	arg1	oil					97:99	cinnamon essential oil	78:99	cinnamon essential oil	78:99	Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes.
30703751	3	58	theme	film	645:648	arg1	composite					650:658	zein film composite	640:658	zein film composite	640:658	The results showed that the combination of CEO-CNPs significantly improves the tensile strength and decreases the elongation of zein film composite.
30703751	5	59	theme	light	957:961	arg1	DLS					975:977	DLS	975:977	DLS	975:977	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	59	theme	light	957:961	arg1	scattering					963:972	dynamic light scattering	949:972	dynamic light scattering (DLS)	949:978	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	5	60	theme	distribution	848:859	arg1	size					817:820	Nano-scale size	806:820	Nano-scale size of CNPs and their uniform distribution within the zein film	806:880	Nano-scale size of CNPs and their uniform distribution within the zein film were monitored by scanning electron microscopy (SEM) and proved by dynamic light scattering (DLS) and transmission electron microscopy (TEM).
30703751	7	61	theme	potential	1424:1432	arg1	materials					1458:1466	potential degradable film-forming materials	1424:1466	potential degradable film-forming materials for food packaging applications	1424:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	7	61	theme	potential	1424:1432	arg1	composites					1391:1400	the reinforced zein based composites	1365:1400	the reinforced zein based composites	1365:1400	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	3	62	theme	tensile	591:597	arg1	strength					599:606	the tensile strength	587:606	the tensile strength	587:606	The results showed that the combination of CEO-CNPs significantly improves the tensile strength and decreases the elongation of zein film composite.
30703751	7	63	theme	food	1472:1475	arg1	applications					1487:1498	food packaging applications	1472:1498	food packaging applications	1472:1498	Thus, it can be concluded that the reinforced zein based composites could be suggested as potential degradable film-forming materials for food packaging applications.
30703751	2	64	theme	mechanical	410:419	arg1	properties					451:460	The physical, mechanical, structural and antibacterial properties	396:460	The physical, mechanical, structural and antibacterial properties of the enriched zein films	396:487	The physical, mechanical, structural and antibacterial properties of the enriched zein films were also scrutinized.
30703751	6	65	with	combination	1210:1220	arg1	CNPs					1227:1230	CNPs	1227:1230	CNPs in zein films	1227:1244	The antimicrobial properties were investigated against Escherichia coli and Staphylococcus aureus, observing that their growth was considerably inhibited by addition of CEO alone and in combination with CNPs in zein films, while CNPs-loaded zein film had no significant effect on the growth of microorganisms.
30175584	2	0	theme	X-ray	485:489	arg1	XPS					519:521	XPS	519:521	XPS	519:521	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	2	0	theme	X-ray	485:489	arg1	spectroscopy					505:516	X-ray photoelectron spectroscopy	485:516	X-ray photoelectron spectroscopy (XPS)	485:522	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	7	1	theme	CNC-TFC	1225:1231	arg1	membranes					1233:1241	the CNC-TFC membranes	1221:1241	the CNC-TFC membranes	1221:1241	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	2	2	dep	transform	449:457	arg1	infrared					459:466	infrared	459:466	transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS)	449:522	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	9	3	theme	CNC-TFC	1513:1519	arg1	membranes					1521:1529	the CNC-TFC membranes	1509:1529	the CNC-TFC membranes	1509:1529	In addition, the CNC-TFC membranes exhibited enhanced antifouling properties due to their increased hydrophilicity and more negatively charged surfaces.
30175584	1	4	from	properties	248:257	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	3	5	theme	control	712:718	arg1	CNC-TFC-0					730:738	CNC-TFC-0	730:738	CNC-TFC-0	730:738	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	3	5	theme	control	712:718	arg1	membrane					720:727	a control membrane	710:727	a control membrane (CNC-TFC-0)	710:739	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	0	6	theme	Antifouling	166:176	arg1	Properties					178:187	Antifouling Properties	166:187	Antifouling Properties	166:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	1	7	theme	thin-film	264:272	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	0	8	theme	Composite	85:93	arg1	Membranes					116:124	Thin-Film Composite (TFC) Nanofiltration Membranes	75:124	Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	75:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	7	9	theme	CNC-TFC-0	1280:1288	arg1	membrane					1290:1297	the CNC-TFC-0 membrane	1276:1297	the CNC-TFC-0 membrane	1276:1297	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	6	10	theme	polyamide	1161:1169	arg1	TFC					1171:1173	the polyamide TFC	1157:1173	the polyamide TFC	1157:1173	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	1	11	theme	composite	274:282	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	0	12	theme	Thin-Film	75:83	arg1	Membranes					116:124	Thin-Film Composite (TFC) Nanofiltration Membranes	75:124	Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	75:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	9	13	theme	increased	1586:1594	arg1	hydrophilicity					1596:1609	their increased hydrophilicity	1580:1609	their increased hydrophilicity	1580:1609	In addition, the CNC-TFC membranes exhibited enhanced antifouling properties due to their increased hydrophilicity and more negatively charged surfaces.
30175584	4	14	theme	smaller	912:918	arg1	MWCO					920:923	a smaller MWCO	910:923	a smaller MWCO	910:923	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	4	15	theme	cutoff	796:801	arg1	characterizations					810:826	molecular weight cutoff (MWCO) characterizations	779:826	molecular weight cutoff (MWCO) characterizations	779:826	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	5	16	theme	CNC-TFC	959:965	arg1	membranes					967:975	The CNC-TFC membranes	955:975	The CNC-TFC membranes	955:975	The CNC-TFC membranes showed enhanced hydrophilicity and increased permeability.
30175584	0	17	theme	TFC	96:98	arg1	Membranes					116:124	Thin-Film Composite (TFC) Nanofiltration Membranes	75:124	Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	75:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	4	18	theme	negative	882:889	arg1	charge					899:904	a greater negative surface charge	872:904	a greater negative surface charge	872:904	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	9	19	theme	antifouling	1550:1560	arg1	properties					1562:1571	enhanced antifouling properties	1541:1571	enhanced antifouling properties	1541:1571	In addition, the CNC-TFC membranes exhibited enhanced antifouling properties due to their increased hydrophilicity and more negatively charged surfaces.
30175584	3	20	from	differences	639:649	arg1	morphologies					658:669	the morphologies	654:669	the morphologies of the CNC-TFC membranes	654:694	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	6	21	theme	membrane	1116:1123	arg1	permeability					1088:1099	the permeability	1084:1099	the permeability of the CNC-TFC membrane	1084:1123	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	7	22	theme	MgSO4	1212:1216	arg1	Rejection					1188:1196	Rejection	1188:1196	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes	1188:1241	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	1	23	theme	polyamide	372:380	arg1	layer					382:386	the polyamide layer	368:386	the polyamide layer of a TFC membrane for the first time	368:423	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	4	24	theme	greater	874:880	arg1	charge					899:904	a greater negative surface charge	872:904	a greater negative surface charge	872:904	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	2	25	theme	layer	590:594	arg1	formation					549:557	the successful formation	534:557	the successful formation of the CNC-polyamide composite layer	534:594	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	6	26	theme	CNC-TFC	1108:1114	arg1	membrane					1116:1123	the CNC-TFC membrane	1104:1123	the CNC-TFC membrane	1104:1123	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	3	27	theme	membranes	686:694	arg1	morphologies					658:669	the morphologies	654:669	the morphologies of the CNC-TFC membranes	654:694	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	2	28	theme	composite	580:588	arg1	layer					590:594	the CNC-polyamide composite layer	562:594	the CNC-polyamide composite layer	562:594	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	7	29	theme	Na2SO4	1201:1206	arg1	Rejection					1188:1196	Rejection	1188:1196	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes	1188:1241	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	1	30	theme	TFC	285:287	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	4	31	theme	surface	891:897	arg1	charge					899:904	a greater negative surface charge	872:904	a greater negative surface charge	872:904	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	2	32	dep	Fourier	441:447	arg1	transform					449:457	transform	449:457	transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS)	449:522	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	0	33	theme	Nanocrystals	27:38	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.	0:188	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	8	34	theme	ion	1433:1435	arg1	rejection					1437:1445	The monovalent ion rejection	1418:1445	The monovalent ion rejection	1418:1445	The monovalent ion rejection tended to increase as the CNC content increased.
30175584	0	35	theme	Nanofiltration	101:114	arg1	Membranes					116:124	Thin-Film Composite (TFC) Nanofiltration Membranes	75:124	Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	75:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	4	36	theme	weight	789:794	arg1	MWCO					804:807	MWCO	804:807	MWCO	804:807	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	4	36	theme	weight	789:794	arg1	cutoff					796:801	molecular weight cutoff	779:801	molecular weight cutoff (MWCO) characterizations	779:826	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	7	37	theme	divalent	1374:1381	arg1	rejection					1388:1396	divalent salt rejection	1374:1396	divalent salt rejection	1374:1396	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	0	38	theme	Cellulose	17:25	arg1	CNCs					41:44	CNCs	41:44	CNCs	41:44	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	0	38	theme	Cellulose	17:25	arg1	Nanocrystals					27:38	Cellulose Nanocrystals	17:38	Cellulose Nanocrystals (CNCs)	17:45	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	1	39	theme	nanofiltration	290:303	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	7	40	from	values	1303:1308	arg1	similar					1247:1253	similar	1247:1253	similar	1247:1253	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	4	41	theme	molecular	779:787	arg1	MWCO					804:807	MWCO	804:807	MWCO	804:807	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	4	41	theme	molecular	779:787	arg1	cutoff					796:801	molecular weight cutoff	779:801	molecular weight cutoff (MWCO) characterizations	779:826	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	1	42	theme	greater	201:207	arg1	performance					220:230	greater separation performance	201:230	greater separation performance	201:230	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	2	43	theme	successful	538:547	arg1	formation					549:557	the successful formation	534:557	the successful formation of the CNC-polyamide composite layer	534:594	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	3	44	theme	CNC-TFC	678:684	arg1	membranes					686:694	the CNC-TFC membranes	674:694	the CNC-TFC membranes	674:694	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	3	45	theme	characterization	605:620	arg1	results					622:628	Surface characterization results	597:628	Surface characterization results	597:628	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	7	46	theme	%	1331:1331	arg1	values					1303:1308	values	1303:1308	values of approximately 98.7% and 98.8%, respectively	1303:1355	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	1	47	theme	separation	209:218	arg1	performance					220:230	greater separation performance	201:230	greater separation performance	201:230	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	0	48	theme	Membranes	116:124	arg1	Layer					66:70	the Polyamide Layer	52:70	the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	52:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	1	49	theme	TFC	393:395	arg1	membrane					397:404	a TFC membrane	391:404	a TFC membrane for the first time	391:423	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	4	50	theme	CNC-TFC	844:850	arg1	membranes					852:860	the CNC-TFC membranes	840:860	the CNC-TFC membranes	840:860	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	6	51	theme	CNCs	1078:1081	arg1	incorporation					1045:1057	the incorporation	1041:1057	the incorporation of only 0.020 wt % CNCs	1041:1081	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	1	52	theme	cellulose	315:323	arg1	CNCs					339:342	CNCs	339:342	CNCs	339:342	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	1	52	theme	cellulose	315:323	arg1	nanocrystals					325:336	cellulose nanocrystals	315:336	cellulose nanocrystals (CNCs)	315:343	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	0	53	theme	Separation	139:148	arg1	Performance					150:160	Enhanced Separation Performance	130:160	Enhanced Separation Performance	130:160	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	1	54	theme	membrane	397:404	arg1	layer					382:386	the polyamide layer	368:386	the polyamide layer of a TFC membrane for the first time	368:423	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	5	55	theme	increased	1012:1020	arg1	permeability					1022:1033	increased permeability	1012:1033	increased permeability	1012:1033	The CNC-TFC membranes showed enhanced hydrophilicity and increased permeability.
30175584	1	56	from	performance	220:230	arg1	membrane					305:312	a thin-film composite (TFC) nanofiltration membrane	262:312	a thin-film composite (TFC) nanofiltration membrane	262:312	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	2	57	theme	Fourier	441:447	arg1	results					430:436	The results	426:436	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS)	426:522	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	0	58	theme	Enhanced	130:137	arg1	Performance					150:160	Enhanced Separation Performance	130:160	Enhanced Separation Performance	130:160	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	3	59	theme	Surface	597:603	arg1	results					622:628	Surface characterization results	597:628	Surface characterization results	597:628	Surface characterization results revealed differences in the morphologies of the CNC-TFC membranes compared with a control membrane (CNC-TFC-0).
30175584	2	60	theme	CNC-polyamide	566:578	arg1	layer					590:594	the CNC-polyamide composite layer	562:594	the CNC-polyamide composite layer	562:594	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	9	61	theme	charged	1631:1637	arg1	surfaces					1639:1646	more negatively charged surfaces	1615:1646	more negatively charged surfaces	1615:1646	In addition, the CNC-TFC membranes exhibited enhanced antifouling properties due to their increased hydrophilicity and more negatively charged surfaces.
30175584	6	62	theme	wt	1073:1074	arg1	CNCs					1078:1081	only 0.020 wt % CNCs	1062:1081	only 0.020 wt % CNCs	1062:1081	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	4	63	theme	potential	752:760	arg1	measurements					762:773	Streaming potential measurements	742:773	Streaming potential measurements	742:773	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	5	64	theme	enhanced	984:991	arg1	hydrophilicity					993:1006	enhanced hydrophilicity	984:1006	enhanced hydrophilicity	984:1006	The CNC-TFC membranes showed enhanced hydrophilicity and increased permeability.
30175584	6	65	theme	%	1076:1076	arg1	CNCs					1078:1081	only 0.020 wt % CNCs	1062:1081	only 0.020 wt % CNCs	1062:1081	With the incorporation of only 0.020 wt % CNCs, the permeability of the CNC-TFC membrane increased by 60.0% over that of the polyamide TFC without CNC.
30175584	4	66	theme	CNC	932:934	arg1	content					936:942	the CNC content	928:942	the CNC content	928:942	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	1	67	theme	first	414:418	arg1	time					420:423	the first time	410:423	the first time	410:423	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	4	68	theme	Streaming	742:750	arg1	measurements					762:773	Streaming potential measurements	742:773	Streaming potential measurements	742:773	Streaming potential measurements and molecular weight cutoff (MWCO) characterizations showed that the CNC-TFC membranes exhibited a greater negative surface charge and a smaller MWCO as the CNC content increased.
30175584	1	69	theme	antifouling	236:246	arg1	properties					248:257	antifouling properties	236:257	antifouling properties	236:257	To achieve greater separation performance and antifouling properties in a thin-film composite (TFC) nanofiltration membrane, cellulose nanocrystals (CNCs) were incorporated into the polyamide layer of a TFC membrane for the first time.
30175584	0	70	theme	Polyamide	56:64	arg1	Layer					66:70	the Polyamide Layer	52:70	the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties	52:187	Incorporation of Cellulose Nanocrystals (CNCs) into the Polyamide Layer of Thin-Film Composite (TFC) Nanofiltration Membranes for Enhanced Separation Performance and Antifouling Properties.
30175584	8	71	theme	CNC	1473:1475	arg1	content					1477:1483	the CNC content	1469:1483	the CNC content	1469:1483	The monovalent ion rejection tended to increase as the CNC content increased.
30175584	7	72	theme	%	1341:1341	arg1	values					1303:1308	values	1303:1308	values of approximately 98.7% and 98.8%, respectively	1303:1355	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30175584	9	73	theme	enhanced	1541:1548	arg1	properties					1562:1571	enhanced antifouling properties	1541:1571	enhanced antifouling properties	1541:1571	In addition, the CNC-TFC membranes exhibited enhanced antifouling properties due to their increased hydrophilicity and more negatively charged surfaces.
30175584	2	74	theme	photoelectron	491:503	arg1	XPS					519:521	XPS	519:521	XPS	519:521	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	2	74	theme	photoelectron	491:503	arg1	spectroscopy					505:516	X-ray photoelectron spectroscopy	485:516	X-ray photoelectron spectroscopy (XPS)	485:522	The results of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the CNC-polyamide composite layer.
30175584	8	75	theme	monovalent	1422:1431	arg1	rejection					1437:1445	The monovalent ion rejection	1418:1445	The monovalent ion rejection	1418:1445	The monovalent ion rejection tended to increase as the CNC content increased.
30175584	7	76	theme	salt	1383:1386	arg1	rejection					1388:1396	divalent salt rejection	1374:1396	divalent salt rejection	1374:1396	Rejection of Na2SO4 and MgSO4 by the CNC-TFC membranes was similar to that observed for the CNC-TFC-0 membrane, at values of approximately 98.7% and 98.8%, respectively, indicating that divalent salt rejection was not sacrificed.
30476515	3	0	theme	isolate	327:333	arg1	gelation					302:309	the heat-induced gelation	285:309	the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	285:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	4	1	theme	BC	475:476	arg1	microfibrils					478:489	BC microfibrils	475:489	BC microfibrils	475:489	Our results showed that WPI and BC microfibrils form a homogeneous dispersion at pH 7.
30476515	5	2	theme	microfibril	607:617	arg1	gel					619:621	the BC microfibril gel	600:621	the BC microfibril gel	600:621	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	7	3	theme	certain	938:944	arg1	value					946:950	above a certain value	930:950	above a certain value	930:950	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	7	3	theme	certain	938:944	arg1	strength					903:910	the strength	899:910	the strength of the WPI gel	899:925	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	7	4	theme	storage	828:834	arg1	modulus					836:842	the storage modulus	824:842	the storage modulus of the WPI gel	824:857	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	5	5	theme	composite	656:664	arg1	gel					666:668	a composite gel	654:668	a composite gel	654:668	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	9	6	theme	microfibril	1186:1196	arg1	gels					1198:1201	the WPI-BC microfibril gels	1175:1201	the WPI-BC microfibril gels	1175:1201	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	5	7	theme	gel	619:621	arg1	presence					588:595	the presence	584:595	the presence of the BC microfibril gel	584:621	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	6	8	theme	BC	756:757	arg1	microfibrils					759:770	BC microfibrils	756:770	BC microfibrils	756:770	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	1	9	theme	appropriate	160:170	arg1	conditions					172:181	appropriate conditions	160:181	appropriate conditions	160:181	Whey proteins can form different gel structures ranging from fine-stranded to particulate when appropriate conditions are applied.
30476515	7	10	theme	microfibrils	801:812	arg1	presence					786:793	the presence	782:793	the presence of BC microfibrils	782:812	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	10	11	theme	water	1259:1263	arg1	capacity					1273:1280	water holding capacity	1259:1280	water holding capacity	1259:1280	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	3	12	from	pH 7	401:404	arg1	microfibrils					385:396	bacterial cellulose (BC) microfibrils	360:396	bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	360:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	12	from	pH 7	401:404	arg1	presence					348:355	the presence	344:355	the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	344:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	10	13	theme	WPI-BC	1289:1294	arg1	gels					1314:1317	the WPI-BC microfibril mixed gels	1285:1317	the WPI-BC microfibril mixed gels	1285:1317	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	1	14	dep	particulate	143:153	arg1	to					140:141	to	140:141	to	140:141	Whey proteins can form different gel structures ranging from fine-stranded to particulate when appropriate conditions are applied.
30476515	8	15	theme	particulate	1047:1057	arg1	gel					1059:1061	a particulate gel	1045:1061	a particulate gel	1045:1061	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	8	16	theme	gel	1001:1003	arg1	structure					1005:1013	the WPI gel structure	993:1013	the WPI gel structure	993:1013	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	7	17	theme	WPI	851:853	arg1	gel					855:857	the WPI gel	847:857	the WPI gel	847:857	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	1	18	theme	Whey	65:68	arg1	proteins					70:77	Whey proteins	65:77	Whey proteins	65:77	Whey proteins can form different gel structures ranging from fine-stranded to particulate when appropriate conditions are applied.
30476515	7	19	theme	BC	798:799	arg1	microfibrils					801:812	BC microfibrils	798:812	BC microfibrils	798:812	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	0	20	theme	Mixed	0:4	arg1	gels					6:9	Mixed gels	0:9	Mixed gels from whey protein	0:27	Mixed gels from whey protein isolate and cellulose microfibrils.
30476515	8	21	theme	BC	1074:1075	arg1	structure					1093:1101	the BC microfibril gel structure	1070:1101	the BC microfibril gel structure	1070:1101	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	0	22	from	protein	21:27	arg1	gels					6:9	Mixed gels	0:9	Mixed gels from whey protein	0:27	Mixed gels from whey protein isolate and cellulose microfibrils.
30476515	5	23	theme	WPI	548:550	arg1	gel					552:554	the WPI gel	544:554	the WPI gel	544:554	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	8	24	theme	WPI	997:999	arg1	structure					1005:1013	the WPI gel structure	993:1013	the WPI gel structure	993:1013	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	3	25	theme	BC	381:382	arg1	microfibrils					385:396	bacterial cellulose (BC) microfibrils	360:396	bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	360:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	5	26	theme	BC	604:605	arg1	gel					619:621	the BC microfibril gel	600:621	the BC microfibril gel	600:621	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	7	27	theme	gel	855:857	arg1	modulus					836:842	the storage modulus	824:842	the storage modulus of the WPI gel	824:857	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	3	28	theme	different	409:417	arg1	concentrations					419:432	different concentrations	409:432	different concentrations of NaCl	409:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	0	29	theme	whey	16:19	arg1	protein					21:27	whey protein	16:27	whey protein	16:27	Mixed gels from whey protein isolate and cellulose microfibrils.
30476515	10	30	theme	gels	1314:1317	arg1	properties					1244:1253	the rheological properties	1228:1253	the rheological properties	1228:1253	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	10	30	theme	gels	1314:1317	arg1	capacity					1273:1280	water holding capacity	1259:1280	water holding capacity	1259:1280	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	8	31	theme	concentration	978:990	arg1	increase					961:968	an increase	958:968	an increase of NaCl concentration	958:990	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	8	32	theme	gel	1089:1091	arg1	structure					1093:1101	the BC microfibril gel structure	1070:1101	the BC microfibril gel structure	1070:1101	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	1	33	theme	different	88:96	arg1	structures					102:111	different gel structures	88:111	different gel structures ranging from fine-stranded to particulate	88:153	Whey proteins can form different gel structures ranging from fine-stranded to particulate when appropriate conditions are applied.
30476515	10	34	theme	microfibril	1296:1306	arg1	gels					1314:1317	the WPI-BC microfibril mixed gels	1285:1317	the WPI-BC microfibril mixed gels	1285:1317	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	3	35	theme	cellulose	370:378	arg1	microfibrils					385:396	bacterial cellulose (BC) microfibrils	360:396	bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	360:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	36	theme	heat-induced	289:300	arg1	gelation					302:309	the heat-induced gelation	285:309	the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	285:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	1	37	theme	gel	98:100	arg1	structures					102:111	different gel structures	88:111	different gel structures ranging from fine-stranded to particulate	88:153	Whey proteins can form different gel structures ranging from fine-stranded to particulate when appropriate conditions are applied.
30476515	9	38	theme	WPI-BC	1179:1184	arg1	gels					1198:1201	the WPI-BC microfibril gels	1175:1201	the WPI-BC microfibril gels	1175:1201	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	5	39	theme	gel	666:668	arg1	formation					641:649	the formation	637:649	the formation of a composite gel	637:668	Upon heating, the WPI gel was formed independently in the presence of the BC microfibril gel, resulting in the formation of a composite gel.
30476515	9	40	theme	macroscopic	1125:1135	arg1	separation					1143:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	2	41	theme	WPI	246:248	arg1	gelation					234:241	the gelation	230:241	the gelation of WPI	230:248	By incorporating polysaccharides, the gelation of WPI can be influenced.
30476515	3	42	theme	NaCl	437:440	arg1	concentrations					419:432	different concentrations	409:432	different concentrations of NaCl	409:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	43	from	presence	348:355	arg1	pH 7					401:404	pH 7	401:404	pH 7 at different concentrations of NaCl	401:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	44	theme	bacterial	360:368	arg1	microfibrils					385:396	bacterial cellulose (BC) microfibrils	360:396	bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	360:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	6	45	theme	WPI	714:716	arg1	structure					679:687	gel structure	675:687	gel structure	675:687	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	6	45	theme	WPI	714:716	arg1	dynamics					702:709	gelation dynamics	693:709	gelation dynamics	693:709	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	0	46	theme	cellulose	41:49	arg1	microfibrils					51:62	and cellulose microfibrils	37:62	microfibrils	51:62	Mixed gels from whey protein isolate and cellulose microfibrils.
30476515	4	47	theme	homogeneous	498:508	arg1	dispersion					510:519	a homogeneous dispersion	496:519	a homogeneous dispersion	496:519	Our results showed that WPI and BC microfibrils form a homogeneous dispersion at pH 7.
30476515	3	48	theme	whey	314:317	arg1	WPI					336:338	WPI	336:338	WPI	336:338	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	48	theme	whey	314:317	arg1	isolate					327:333	whey protein isolate	314:333	whey protein isolate (WPI)	314:339	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	10	49	theme	rheological	1232:1242	arg1	properties					1244:1253	the rheological properties	1228:1253	the rheological properties	1228:1253	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	3	50	theme	microfibrils	385:396	arg1	presence					348:355	the presence	344:355	the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	344:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	51	theme	protein	319:325	arg1	WPI					336:338	WPI	336:338	WPI	336:338	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	3	51	theme	protein	319:325	arg1	isolate					327:333	whey protein isolate	314:333	whey protein isolate (WPI)	314:339	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	7	52	theme	WPI	919:921	arg1	gel					923:925	the WPI gel	915:925	the WPI gel	915:925	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	9	53	theme	phase	1137:1141	arg1	separation					1143:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	10	54	theme	mixed	1308:1312	arg1	gels					1314:1317	the WPI-BC microfibril mixed gels	1285:1317	the WPI-BC microfibril mixed gels	1285:1317	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	6	55	theme	gelation	693:700	arg1	dynamics					702:709	gelation dynamics	693:709	gelation dynamics	693:709	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	6	56	theme	gel	675:677	arg1	structure					679:687	gel structure	675:687	gel structure	675:687	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	3	57	from	gelation	302:309	arg1	presence					348:355	the presence	344:355	the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl	344:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	7	58	theme	gel	923:925	arg1	value					946:950	above a certain value	930:950	above a certain value	930:950	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	7	58	theme	gel	923:925	arg1	strength					903:910	the strength	899:910	the strength of the WPI gel	899:925	However, the presence of BC microfibrils increased the storage modulus of the WPI gel, with an increase being negligible when the strength of the WPI gel is above a certain value.
30476515	6	59	theme	microfibrils	759:770	arg1	presence					744:751	the presence	740:751	the presence of BC microfibrils	740:770	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	10	60	theme	holding	1265:1271	arg1	capacity					1273:1280	water holding capacity	1259:1280	water holding capacity	1259:1280	Our results showed that the rheological properties and water holding capacity of the WPI-BC microfibril mixed gels are mainly dominated by the WPI.
30476515	6	61	dep	structure	679:687	arg1	The					671:673	The	671:673	The	671:673	The gel structure and gelation dynamics of WPI was not influenced by the presence of BC microfibrils.
30476515	9	62	located	observed	1163:1170	arg1	gels					1198:1201	the WPI-BC microfibril gels	1175:1201	the WPI-BC microfibril gels	1175:1201	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	9	62	located	observed	1163:1170	arg2	separation					1143:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation	1122:1152	No macroscopic phase separation could be observed in the WPI-BC microfibril gels.
30476515	8	63	theme	microfibril	1077:1087	arg1	structure					1093:1101	the BC microfibril gel structure	1070:1101	the BC microfibril gel structure	1070:1101	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30476515	3	64	from	concentrations	419:432	arg1	pH 7					401:404	pH 7	401:404	pH 7 at different concentrations of NaCl	401:440	We investigated the heat-induced gelation of whey protein isolate (WPI) in the presence of bacterial cellulose (BC) microfibrils at pH 7 at different concentrations of NaCl.
30476515	8	65	theme	NaCl	973:976	arg1	concentration					978:990	NaCl concentration	973:990	NaCl concentration	973:990	With an increase of NaCl concentration, the WPI gel structure changes from fine-stranded to a particulate gel, while the BC microfibril gel structure remains unchanged.
30878203	5	0	theme	two-dimensional	785:799	arg1	substrates					801:810	two-dimensional substrates	785:810	two-dimensional substrates of varying elastic moduli	785:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	6	1	theme	variable	967:974	arg1	composition					976:986	variable composition	967:986	variable composition	967:986	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	2	2	theme	infarction	380:389	arg1	aftermath					354:362	the aftermath	350:362	the aftermath of a myocardial infarction	350:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	5	3	theme	cell	771:774	arg1	types					776:780	other cell types	765:780	other cell types	765:780	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	4	4	theme	cell-surface	595:606	arg1	receptors					608:616	cell-surface receptors	595:616	cell-surface receptors	595:616	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	4	4	theme	cell-surface	595:606	arg1	CD44					629:632	CD44	629:632	CD44	629:632	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	4	4	theme	cell-surface	595:606	arg1	structures					674:683	other surface-exposed structures	652:683	other surface-exposed structures	652:683	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	4	4	theme	cell-surface	595:606	arg1	RHAMM/CD168					635:645	RHAMM/CD168	635:645	RHAMM/CD168	635:645	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	8	5	theme	charge	1275:1280	arg1	density					1282:1288	the same charge density	1266:1288	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	3	6	from	influence	436:444	arg1	flow					492:495	interstitial fluid flow	473:495	interstitial fluid flow	473:495	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	3	6	from	influence	436:444	arg1	response					460:467	mechanical response	449:467	mechanical response	449:467	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	2	7	theme	myocardial	369:378	arg1	infarction					380:389	a myocardial infarction	367:389	a myocardial infarction	367:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	8	8	theme	transmembrane	1306:1318	arg1	receptors					1320:1328	specific transmembrane receptors	1297:1328	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	5	9	theme	cardiomyocytes	746:759	arg1	response					734:741	the response	730:741	the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli	730:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	4	10	contain	has	533:535	arg1	HA					530:531	HA	530:531	HA	530:531	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	4	10	contain	has	533:535	arg2	effect					559:564	a direct, biochemical effect	537:564	a direct, biochemical effect on cells	537:573	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	2	11	theme	early	325:329	arg1	development					331:341	early development	325:341	early development	325:341	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	6	12	dep	composition	976:986	arg1	in vitro					1014:1021	in vitro	1014:1021	in vitro	1014:1021	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	6	13	theme	three-dimensional	937:953	arg1	matrices					955:962	three-dimensional matrices	937:962	three-dimensional matrices of variable composition and mechanical properties in vitro	937:1021	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	6	14	theme	mechanical	992:1001	arg1	properties					1003:1012	mechanical properties	992:1012	mechanical properties	992:1012	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	2	15	theme	myocardium	307:316	arg1	matrix					293:298	the extracellular matrix	275:298	the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction	275:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	4	16	theme	receptors	608:616	arg1	array					586:590	an array	583:590	an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures	583:683	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	5	17	theme	other	765:769	arg1	types					776:780	other cell types	765:780	other cell types	765:780	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	9	18	theme	physical-chemical	1391:1407	arg1	effect					1409:1414	a generic physical-chemical effect	1381:1414	a generic physical-chemical effect	1381:1414	Therefore, these results establish that HA exerts a generic physical-chemical effect within three-dimensional hydrogels that must be accounted for when interrogating cell-matrix interactions.
30878203	1	19	theme	extracellular	85:97	arg1	matrix					99:104	The extracellular matrix	81:104	The extracellular matrix	81:104	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	8	20	theme	same	1270:1273	arg1	density					1282:1288	the same charge density	1266:1288	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	0	21	theme	Fibrin-Based	57:68	arg1	Hydrogels					70:78	3D Fibrin-Based Hydrogels	54:78	3D Fibrin-Based Hydrogels	54:78	Hyaluronan Disrupts Cardiomyocyte Organization within 3D Fibrin-Based Hydrogels.
30878203	6	22	theme	fibroblasts	918:928	arg1	HA					885:886	HA	885:886	HA of cardiomyocytes and cardiac fibroblasts	885:928	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	8	23	with	polysaccharide	1246:1259	arg1	receptors					1320:1328	specific transmembrane receptors	1297:1328	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	8	23	with	polysaccharide	1246:1259	arg1	density					1282:1288	the same charge density	1266:1288	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	1	24	theme	tissue	202:207	arg1	function					209:216	cell and tissue function	193:216	function	209:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	6	25	theme	cardiac	910:916	arg1	fibroblasts					918:928	cardiac fibroblasts	910:928	cardiac fibroblasts	910:928	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	9	26	theme	generic	1383:1389	arg1	effect					1409:1414	a generic physical-chemical effect	1381:1414	a generic physical-chemical effect	1381:1414	Therefore, these results establish that HA exerts a generic physical-chemical effect within three-dimensional hydrogels that must be accounted for when interrogating cell-matrix interactions.
30878203	3	27	theme	mechanical	449:458	arg1	response					460:467	mechanical response	449:467	mechanical response	449:467	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	4	28	theme	direct	539:544	arg1	effect					559:564	a direct, biochemical effect	537:564	a direct, biochemical effect on cells	537:573	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	9	29	theme	three-dimensional	1423:1439	arg1	hydrogels					1441:1449	three-dimensional hydrogels	1423:1449	three-dimensional hydrogels that must be accounted for when interrogating cell-matrix interactions	1423:1520	Therefore, these results establish that HA exerts a generic physical-chemical effect within three-dimensional hydrogels that must be accounted for when interrogating cell-matrix interactions.
30878203	3	30	contain	has	423:425	arg1	structure					413:421	Its flexible anionic structure	392:421	Its flexible anionic structure	392:421	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	3	30	contain	has	423:425	arg2	influence					436:444	a strong influence	427:444	a strong influence on mechanical response and interstitial fluid flow within the matrix	427:513	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	6	31	theme	cardiomyocytes	891:904	arg1	HA					885:886	HA	885:886	HA of cardiomyocytes and cardiac fibroblasts	885:928	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	3	32	theme	flexible	396:403	arg1	structure					413:421	Its flexible anionic structure	392:421	Its flexible anionic structure	392:421	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	8	33	theme	anionic	1238:1244	arg1	alginate					1225:1232	alginate	1225:1232	alginate	1225:1232	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	8	33	theme	anionic	1238:1244	arg1	polysaccharide					1246:1259	an anionic polysaccharide	1235:1259	an anionic polysaccharide with the same charge density but no specific transmembrane receptors	1235:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	7	34	theme	tissue	1160:1165	arg1	gauges					1167:1172	microfabricated tissue gauges	1144:1172	microfabricated tissue gauges	1144:1172	HA significantly decreased the force exerted by the cell-matrix constructs in a tensiometer testing platform and within microfabricated tissue gauges.
30878203	1	35	theme	variable	123:130	arg1	amounts					148:154	variable but often large amounts	123:154	variable but often large amounts of glycosaminoglycans that influence cell and tissue function	123:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	1	35	theme	variable	123:130	arg1	glycosaminoglycans					159:176	glycosaminoglycans	159:176	glycosaminoglycans that influence cell and tissue function	159:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	0	36	theme	Cardiomyocyte	20:32	arg1	Organization					34:45	Cardiomyocyte Organization	20:45	Cardiomyocyte Organization	20:45	Hyaluronan Disrupts Cardiomyocyte Organization within 3D Fibrin-Based Hydrogels.
30878203	1	37	theme	cell	193:196	arg1	function					209:216	cell and tissue function	193:216	function	209:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	2	38	from	matrix	293:298	arg1	aftermath					354:362	the aftermath	350:362	the aftermath of a myocardial infarction	350:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	7	39	from	cell-matrix constructs	1076:1097	arg1	platform					1124:1131	a tensiometer testing platform	1102:1131	a tensiometer testing platform	1102:1131	HA significantly decreased the force exerted by the cell-matrix constructs in a tensiometer testing platform and within microfabricated tissue gauges.
30878203	0	40	theme	3D	54:55	arg1	Hydrogels					70:78	3D Fibrin-Based Hydrogels	54:78	3D Fibrin-Based Hydrogels	54:78	Hyaluronan Disrupts Cardiomyocyte Organization within 3D Fibrin-Based Hydrogels.
30878203	4	41	theme	surface-exposed	658:672	arg1	structures					674:683	other surface-exposed structures	652:683	other surface-exposed structures	652:683	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	3	42	theme	fluid	486:490	arg1	flow					492:495	interstitial fluid flow	473:495	interstitial fluid flow	473:495	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	4	43	from	effect	559:564	arg1	cells					569:573	cells	569:573	cells	569:573	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	4	44	dep	direct	539:544	arg1	biochemical					547:557	biochemical	547:557	biochemical	547:557	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	5	45	theme	varying	815:821	arg1	moduli					831:836	varying elastic moduli	815:836	varying elastic moduli	815:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	8	46	from	that	1217:1220	arg1	different					1202:1210	different	1202:1210	different	1202:1210	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	2	47	theme	abundant	241:248	arg1	glycosaminoglycan					250:266	an abundant glycosaminoglycan	238:266	an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction	238:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	2	47	theme	abundant	241:248	arg1	Hyaluronan					219:228	Hyaluronan	219:228	Hyaluronan (HA)	219:233	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	4	48	theme	other	652:656	arg1	structures					674:683	other surface-exposed structures	652:683	other surface-exposed structures	652:683	Additionally, HA has a direct, biochemical effect on cells through an array of cell-surface receptors, including CD44, RHAMM/CD168, and other surface-exposed structures.
30878203	5	49	theme	elastic	823:829	arg1	moduli					831:836	varying elastic moduli	815:836	varying elastic moduli	815:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	6	50	theme	properties	1003:1012	arg1	matrices					955:962	three-dimensional matrices	937:962	three-dimensional matrices of variable composition and mechanical properties in vitro	937:1021	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	5	51	theme	moduli	831:836	arg1	substrates					801:810	two-dimensional substrates	785:810	two-dimensional substrates of varying elastic moduli	785:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	7	52	theme	microfabricated	1144:1158	arg1	gauges					1167:1172	microfabricated tissue gauges	1144:1172	microfabricated tissue gauges	1144:1172	HA significantly decreased the force exerted by the cell-matrix constructs in a tensiometer testing platform and within microfabricated tissue gauges.
30878203	3	53	theme	strong	429:434	arg1	influence					436:444	a strong influence	427:444	a strong influence on mechanical response and interstitial fluid flow within the matrix	427:513	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	1	54	contain	contains	114:121	arg1	matrix					99:104	The extracellular matrix	81:104	The extracellular matrix	81:104	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	1	54	contain	contains	114:121	arg2	glycosaminoglycans					159:176	glycosaminoglycans	159:176	glycosaminoglycans that influence cell and tissue function	159:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	1	54	contain	contains	114:121	arg2	amounts					148:154	variable but often large amounts	123:154	variable but often large amounts of glycosaminoglycans that influence cell and tissue function	123:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	5	55	theme	Recent	686:691	arg1	studies					693:699	Recent studies	686:699	Recent studies	686:699	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	1	56	theme	large	142:146	arg1	amounts					148:154	variable but often large amounts	123:154	variable but often large amounts of glycosaminoglycans that influence cell and tissue function	123:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	1	56	theme	large	142:146	arg1	glycosaminoglycans					159:176	glycosaminoglycans	159:176	glycosaminoglycans that influence cell and tissue function	159:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	7	57	theme	tensiometer	1104:1114	arg1	platform					1124:1131	a tensiometer testing platform	1102:1131	a tensiometer testing platform	1102:1131	HA significantly decreased the force exerted by the cell-matrix constructs in a tensiometer testing platform and within microfabricated tissue gauges.
30878203	7	58	theme	testing	1116:1122	arg1	platform					1124:1131	a tensiometer testing platform	1102:1131	a tensiometer testing platform	1102:1131	HA significantly decreased the force exerted by the cell-matrix constructs in a tensiometer testing platform and within microfabricated tissue gauges.
30878203	6	59	theme	force	867:871	arg1	response					873:880	the force response	863:880	the force response to HA of cardiomyocytes and cardiac fibroblasts	863:928	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	2	60	theme	extracellular	279:291	arg1	matrix					293:298	the extracellular matrix	275:298	the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction	275:389	Hyaluronan (HA) is an abundant glycosaminoglycan within the extracellular matrix of the myocardium during early development and in the aftermath of a myocardial infarction.
30878203	9	61	theme	cell-matrix	1497:1507	arg1	interactions					1509:1520	cell-matrix interactions	1497:1520	cell-matrix interactions	1497:1520	Therefore, these results establish that HA exerts a generic physical-chemical effect within three-dimensional hydrogels that must be accounted for when interrogating cell-matrix interactions.
30878203	5	62	theme	types	776:780	arg1	response					734:741	the response	730:741	the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli	730:836	Recent studies have shown that HA modulates the response of cardiomyocytes and other cell types to two-dimensional substrates of varying elastic moduli.
30878203	1	63	theme	glycosaminoglycans	159:176	arg1	amounts					148:154	variable but often large amounts	123:154	variable but often large amounts of glycosaminoglycans that influence cell and tissue function	123:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	1	63	theme	glycosaminoglycans	159:176	arg1	glycosaminoglycans					159:176	glycosaminoglycans	159:176	glycosaminoglycans that influence cell and tissue function	159:216	The extracellular matrix in vivo contains variable but often large amounts of glycosaminoglycans that influence cell and tissue function.
30878203	3	64	theme	interstitial	473:484	arg1	flow					492:495	interstitial fluid flow	473:495	interstitial fluid flow	473:495	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30878203	8	65	theme	specific	1297:1304	arg1	receptors					1320:1328	specific transmembrane receptors	1297:1328	the same charge density but no specific transmembrane receptors	1266:1328	However, its effect was no different from that of alginate, an anionic polysaccharide with the same charge density but no specific transmembrane receptors.
30878203	6	66	theme	composition	976:986	arg1	matrices					955:962	three-dimensional matrices	937:962	three-dimensional matrices of variable composition and mechanical properties in vitro	937:1021	This study investigates the force response to HA of cardiomyocytes and cardiac fibroblasts within three-dimensional matrices of variable composition and mechanical properties in vitro.
30878203	3	67	theme	anionic	405:411	arg1	structure					413:421	Its flexible anionic structure	392:421	Its flexible anionic structure	392:421	Its flexible anionic structure has a strong influence on mechanical response and interstitial fluid flow within the matrix.
30452995	0	0	theme	N-st	75:78	arg1	films					91:95	PVA/Trapa natans starch (N-st) composite films	50:95	PVA/Trapa natans starch (N-st) composite films	50:95	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	5	1	from	effect	783:788	arg1	biodegradability					872:887	biodegradability	872:887	biodegradability of the composite films	872:910	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	1	from	effect	783:788	arg1	%					864:864	%	864:864	%	864:864	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	1	from	effect	783:788	arg1	properties					838:847	the mechanical properties	823:847	the mechanical properties	823:847	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	1	from	effect	783:788	arg1	uptake					856:861	water uptake	850:861	water uptake (%)	850:865	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	7	2	dep	%	1117:1117	arg1	to					1112:1113	to	1112:1113	to	1112:1113	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	7	2	dep	%	1117:1117	arg1	from					1102:1105	from	1102:1105	from	1102:1105	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	3	3	dep	transform	536:544	arg1	infrared					546:553	infrared	546:553	infrared	546:553	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	9	4	theme	pure	1390:1393	arg1	3:7					1425:1427	3:7	1425:1427	3:7	1425:1427	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	9	4	theme	pure	1390:1393	arg1	films					1418:1422	pure PVA/N-starch composite films	1390:1422	pure PVA/N-starch composite films (3:7)	1390:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	2	5	theme	monomer	386:392	arg1	concentration					394:406	monomer concentration	386:406	monomer concentration	386:406	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	7	6	theme	further	1054:1060	arg1	increase					1062:1069	further increase	1054:1069	further increase in the ratio of grafted starch from (50% to 70%) Elongation at break	1054:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	10	7	theme	PVA/N-starch-grafted-poly	1478:1502	arg1	films					1526:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	4	8	theme	PVA/N-Starch-grafted-poly	670:694	arg1	films					712:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	6	9	theme	PVA/starch-grafted-poly	952:974	arg1	PVA/starch-grafted-poly					952:974	PVA/starch-grafted-poly	952:974	PVA/starch-grafted-poly (EMA) (1:1)	952:986	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	9	theme	PVA/starch-grafted-poly	952:974	arg1	%					947:947	break %	941:947	break % of PVA/starch-grafted-poly (EMA) (1:1)	941:986	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	9	theme	PVA/starch-grafted-poly	952:974	arg1	EMA					977:979	EMA	977:979	EMA	977:979	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	9	theme	PVA/starch-grafted-poly	952:974	arg1	1:1					983:985	1:1	983:985	1:1	983:985	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	0	10	theme	composite	81:89	arg1	films					91:95	PVA/Trapa natans starch (N-st) composite films	50:95	PVA/Trapa natans starch (N-st) composite films	50:95	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	0	11	from	Studies	0:6	arg1	biodegradability					30:45	biodegradability	30:45	biodegradability	30:45	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	0	11	from	Studies	0:6	arg1	properties					15:24	properties	15:24	properties	15:24	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	6	12	dep	38.9	1004:1007	arg1	to					1001:1002	to	1001:1002	to	1001:1002	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	10	13	theme	composite	1560:1568	arg1	films					1570:1574	PVA/N-starch composite films	1547:1574	PVA/N-starch composite films	1547:1574	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	4	14	theme	N-st	644:647	arg1	films					660:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	9	15	theme	composite	1408:1416	arg1	3:7					1425:1427	3:7	1425:1427	3:7	1425:1427	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	9	15	theme	composite	1408:1416	arg1	films					1418:1422	pure PVA/N-starch composite films	1390:1422	pure PVA/N-starch composite films (3:7)	1390:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	10	16	theme	PVA/N-starch	1547:1558	arg1	films					1570:1574	PVA/N-starch composite films	1547:1574	PVA/N-starch composite films	1547:1574	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	6	17	theme	composites	1031:1040	arg1	%					1008:1008	up to 38.9%	998:1008	up to 38.9% of pure PVA/N-starch composites (1:1)	998:1046	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	17	theme	composites	1031:1040	arg1	1:1					1043:1045	1:1	1043:1045	1:1	1043:1045	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	17	theme	composites	1031:1040	arg1	composites					1031:1040	pure PVA/N-starch composites	1013:1040	pure PVA/N-starch composites (1:1)	1013:1046	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	4	18	theme	composite	702:710	arg1	films					712:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	9	19	dep	100	1326:1328	arg1	to					1323:1324	to	1323:1324	to	1323:1324	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	7	20	theme	grafted	1087:1093	arg1	Elongation					1120:1129	grafted starch from (50% to 70%) Elongation	1087:1129	grafted starch from (50% to 70%) Elongation	1087:1129	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	6	21	theme	pure	1013:1016	arg1	1:1					1043:1045	1:1	1043:1045	1:1	1043:1045	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	21	theme	pure	1013:1016	arg1	composites					1031:1040	pure PVA/N-starch composites	1013:1040	pure PVA/N-starch composites (1:1)	1013:1046	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	9	22	theme	composite	1372:1380	arg1	film					1382:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film	1334:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7)	1334:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	5	23	theme	water	850:854	arg1	%					864:864	%	864:864	%	864:864	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	23	theme	water	850:854	arg1	uptake					856:861	water uptake	850:861	water uptake (%)	850:865	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	1	24	theme	morphological	144:156	arg1	modification					158:169	The morphological modification	140:169	The morphological modification of Trapa natans starch	140:192	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	0	25	theme	films	91:95	arg1	biodegradability					30:45	biodegradability	30:45	biodegradability	30:45	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	0	25	theme	films	91:95	arg1	properties					15:24	properties	15:24	properties	15:24	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	5	26	dep	effect	783:788	arg1	The					779:781	The	779:781	The	779:781	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	10	27	theme	%	1447:1447	arg1	decreases					1449:1457	41.63% decreases	1442:1457	41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1442:1530	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	6	28	from	%	947:947	arg1	Elongation					927:936	Elongation	927:936	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1)	927:986	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	7	29	theme	starch	1095:1100	arg1	Elongation					1120:1129	grafted starch from (50% to 70%) Elongation	1087:1129	grafted starch from (50% to 70%) Elongation	1087:1129	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	5	30	from	content	794:800	arg1	biodegradability					872:887	biodegradability	872:887	biodegradability of the composite films	872:910	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	30	from	content	794:800	arg1	%					864:864	%	864:864	%	864:864	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	30	from	content	794:800	arg1	properties					838:847	the mechanical properties	823:847	the mechanical properties	823:847	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	30	from	content	794:800	arg1	uptake					856:861	water uptake	850:861	water uptake (%)	850:865	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	6	31	theme	break	941:945	arg1	PVA/starch-grafted-poly					952:974	PVA/starch-grafted-poly	952:974	PVA/starch-grafted-poly (EMA) (1:1)	952:986	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	31	theme	break	941:945	arg1	%					947:947	break %	941:947	break % of PVA/starch-grafted-poly (EMA) (1:1)	941:986	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	31	theme	break	941:945	arg1	EMA					977:979	EMA	977:979	EMA	977:979	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	31	theme	break	941:945	arg1	1:1					983:985	1:1	983:985	1:1	983:985	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	5	32	theme	composite	896:904	arg1	films					906:910	the composite films	892:910	the composite films	892:910	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	4	33	theme	composite	650:658	arg1	films					660:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	1	34	theme	ferrous	250:256	arg1	persulphate					286:296	ferrous ammonium sulphate‑potassium persulphate	250:296	ferrous ammonium sulphate‑potassium persulphate	250:296	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	1	34	theme	ferrous	250:256	arg1	initiator					309:317	a redox initiator	301:317	a redox initiator	301:317	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	5	35	theme	films	906:910	arg1	biodegradability					872:887	biodegradability	872:887	biodegradability of the composite films	872:910	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	35	theme	films	906:910	arg1	%					864:864	%	864:864	%	864:864	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	35	theme	films	906:910	arg1	properties					838:847	the mechanical properties	823:847	the mechanical properties	823:847	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	35	theme	films	906:910	arg1	uptake					856:861	water uptake	850:861	water uptake (%)	850:865	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	1	36	theme	Trapa	174:178	arg1	starch					187:192	Trapa natans starch	174:192	Trapa natans starch	174:192	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	1	37	theme	ammonium	258:265	arg1	persulphate					286:296	ferrous ammonium sulphate‑potassium persulphate	250:296	ferrous ammonium sulphate‑potassium persulphate	250:296	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	1	37	theme	ammonium	258:265	arg1	initiator					309:317	a redox initiator	301:317	a redox initiator	301:317	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	8	38	theme	pure	1254:1257	arg1	films					1288:1292	pure PVA/N-starch (3:7) composite films	1254:1292	pure PVA/N-starch (3:7) composite films	1254:1292	There was 223.14% increased in PVA/starch-grafted-poly (EMA) (3:7) as compared to pure PVA/N-starch (3:7) composite films.
30452995	9	39	theme	PVA/N-starch	1395:1406	arg1	3:7					1425:1427	3:7	1425:1427	3:7	1425:1427	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	9	39	theme	PVA/N-starch	1395:1406	arg1	films					1418:1422	pure PVA/N-starch composite films	1390:1422	pure PVA/N-starch composite films (3:7)	1390:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	9	40	theme	films	1418:1422	arg1	film					1382:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film	1334:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7)	1334:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	1	41	theme	natans	180:185	arg1	starch					187:192	Trapa natans starch	174:192	Trapa natans starch	174:192	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	5	42	theme	grafted	805:811	arg1	starch					813:818	grafted starch	805:818	grafted starch	805:818	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	1	43	theme	sulphate‑potassium	267:284	arg1	persulphate					286:296	ferrous ammonium sulphate‑potassium persulphate	250:296	ferrous ammonium sulphate‑potassium persulphate	250:296	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	1	43	theme	sulphate‑potassium	267:284	arg1	initiator					309:317	a redox initiator	301:317	a redox initiator	301:317	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	0	44	theme	PVA/N-st-g-poly	101:115	arg1	films					133:137	PVA/N-st-g-poly (EMA) composite films	101:137	PVA/N-st-g-poly (EMA) composite films	101:137	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	6	45	theme	PVA/N-starch	1018:1029	arg1	1:1					1043:1045	1:1	1043:1045	1:1	1043:1045	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	6	45	theme	PVA/N-starch	1018:1029	arg1	composites					1031:1040	pure PVA/N-starch composites	1013:1040	pure PVA/N-starch composites (1:1)	1013:1046	Elongation at break % of PVA/starch-grafted-poly (EMA) (1:1) increased up to 38.9% of pure PVA/N-starch composites (1:1).
30452995	1	46	theme	starch	187:192	arg1	modification					158:169	The morphological modification	140:169	The morphological modification of Trapa natans starch	140:192	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	10	47	theme	41.63	1442:1446	arg1	%					1447:1447	%	1447:1447	%	1447:1447	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	4	48	theme	EMA	697:699	arg1	films					712:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/N-Starch-grafted-poly (EMA) composite films	670:716	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	3	49	theme	electron	565:572	arg1	microscopy					574:583	scanning electron microscopy	556:583	scanning electron microscopy	556:583	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	9	50	theme	Max	1299:1301	arg1	stress					1303:1308	The Max stress	1295:1308	The Max stress	1295:1308	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	4	51	theme	PVA/starch	632:641	arg1	films					660:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films	632:664	PVA/starch (N-st) composite films and PVA/N-Starch-grafted-poly (EMA) composite films were prepared separately by using glycerol as a plasticizer.
30452995	2	52	theme	graft	454:458	arg1	%					469:469	56%	467:469	56%	467:469	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	52	theme	graft	454:458	arg1	yield					460:464	maximum graft yield	446:464	maximum graft yield (56%)	446:470	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	3	53	theme	TGA/DTA/DTG	608:618	arg1	techniques					620:629	TGA/DTA/DTG techniques	608:629	TGA/DTA/DTG techniques	608:629	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	10	54	from	decreases	1449:1457	arg1	films					1526:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	10	54	from	decreases	1449:1457	arg1	uptake					1468:1473	water uptake	1462:1473	water uptake	1462:1473	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	5	55	theme	mechanical	827:836	arg1	properties					838:847	the mechanical properties	823:847	the mechanical properties	823:847	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	2	56	theme	maximum	446:452	arg1	%					469:469	56%	467:469	56%	467:469	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	56	theme	maximum	446:452	arg1	yield					460:464	maximum graft yield	446:464	maximum graft yield (56%)	446:470	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	7	57	theme	%	1110:1110	arg1	%					1117:1117	50% to 70%	1108:1117	50% to 70%	1108:1117	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	7	57	theme	%	1110:1110	arg1	starch					1095:1100	starch	1095:1100	grafted starch from (50% to 70%) Elongation	1087:1129	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	0	58	theme	films	133:137	arg1	biodegradability					30:45	biodegradability	30:45	biodegradability	30:45	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	0	58	theme	films	133:137	arg1	properties					15:24	properties	15:24	properties	15:24	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	0	59	theme	natans	60:65	arg1	films					91:95	PVA/Trapa natans starch (N-st) composite films	50:95	PVA/Trapa natans starch (N-st) composite films	50:95	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	8	60	theme	PVA/N-starch	1259:1270	arg1	films					1288:1292	pure PVA/N-starch (3:7) composite films	1254:1292	pure PVA/N-starch (3:7) composite films	1254:1292	There was 223.14% increased in PVA/starch-grafted-poly (EMA) (3:7) as compared to pure PVA/N-starch (3:7) composite films.
30452995	9	61	theme	PVA/N-starch-grafted-poly	1334:1358	arg1	film					1382:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film	1334:1385	PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7)	1334:1428	The Max stress increased up to 100% in PVA/N-starch-grafted-poly (EMA) (3:7) composite film of pure PVA/N-starch composite films (3:7).
30452995	1	62	theme	redox	303:307	arg1	persulphate					286:296	ferrous ammonium sulphate‑potassium persulphate	250:296	ferrous ammonium sulphate‑potassium persulphate	250:296	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	1	62	theme	redox	303:307	arg1	initiator					309:317	a redox initiator	301:317	a redox initiator	301:317	The morphological modification of Trapa natans starch was done by grafting the methylmethacrylate (EMA) using ferrous ammonium sulphate‑potassium persulphate as a redox initiator.
30452995	0	63	theme	composite	123:131	arg1	films					133:137	PVA/N-st-g-poly (EMA) composite films	101:137	PVA/N-st-g-poly (EMA) composite films	101:137	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	3	64	theme	scanning	556:563	arg1	microscopy					574:583	scanning electron microscopy	556:583	scanning electron microscopy	556:583	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	2	65	theme	reaction	330:337	arg1	solvent					416:422	solvent	416:422	solvent	416:422	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	65	theme	reaction	330:337	arg1	concentration					394:406	monomer concentration	386:406	monomer concentration	386:406	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	65	theme	reaction	330:337	arg1	temperature					367:377	reaction temperature	358:377	reaction temperature	358:377	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	65	theme	reaction	330:337	arg1	pH					409:410	pH	409:410	pH	409:410	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	65	theme	reaction	330:337	arg1	parameters					339:348	Different reaction parameters	320:348	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent	320:422	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	65	theme	reaction	330:337	arg1	time					380:383	time	380:383	time	380:383	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	8	66	theme	3:7	1273:1275	arg1	films					1288:1292	pure PVA/N-starch (3:7) composite films	1254:1292	pure PVA/N-starch (3:7) composite films	1254:1292	There was 223.14% increased in PVA/starch-grafted-poly (EMA) (3:7) as compared to pure PVA/N-starch (3:7) composite films.
30452995	0	67	dep	properties	15:24	arg1	the					11:13	the	11:13	the	11:13	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	7	68	from	break	1134:1138	arg1	increase					1062:1069	further increase	1054:1069	further increase in the ratio of grafted starch from (50% to 70%) Elongation at break	1054:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	7	68	from	break	1134:1138	arg1	ratio					1078:1082	the ratio	1074:1082	the ratio of grafted starch from (50% to 70%) Elongation at break	1074:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	2	69	theme	Different	320:328	arg1	solvent					416:422	solvent	416:422	solvent	416:422	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	69	theme	Different	320:328	arg1	concentration					394:406	monomer concentration	386:406	monomer concentration	386:406	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	69	theme	Different	320:328	arg1	temperature					367:377	reaction temperature	358:377	reaction temperature	358:377	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	69	theme	Different	320:328	arg1	pH					409:410	pH	409:410	pH	409:410	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	69	theme	Different	320:328	arg1	parameters					339:348	Different reaction parameters	320:348	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent	320:422	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	2	69	theme	Different	320:328	arg1	time					380:383	time	380:383	time	380:383	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
30452995	10	70	theme	composite	1516:1524	arg1	films					1526:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	PVA/N-starch-grafted-poly (EMA) (3:7) composite films	1478:1530	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	7	71	theme	great	1158:1162	arg1	extent					1164:1169	a great extent	1156:1169	a great extent	1156:1169	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	3	72	dep	Fourier	528:534	arg1	transform					536:544	transform	536:544	transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques	536:629	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	0	73	theme	starch	67:72	arg1	films					91:95	PVA/Trapa natans starch (N-st) composite films	50:95	PVA/Trapa natans starch (N-st) composite films	50:95	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	8	74	theme	composite	1278:1286	arg1	films					1288:1292	pure PVA/N-starch (3:7) composite films	1254:1292	pure PVA/N-starch (3:7) composite films	1254:1292	There was 223.14% increased in PVA/starch-grafted-poly (EMA) (3:7) as compared to pure PVA/N-starch (3:7) composite films.
30452995	5	75	theme	starch	813:818	arg1	content					794:800	content	794:800	content	794:800	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	5	75	theme	starch	813:818	arg1	effect					783:788	effect	783:788	effect	783:788	The effect and content of grafted starch on the mechanical properties, water uptake (%), and biodegradability of the composite films were observed.
30452995	3	76	theme	graft	477:481	arg1	copolymers					483:492	The graft copolymers	473:492	The graft copolymers thus formed	473:504	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	7	77	theme	Elongation	1120:1129	arg1	ratio					1078:1082	the ratio	1074:1082	the ratio of grafted starch from (50% to 70%) Elongation at break	1074:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	10	78	theme	water	1462:1466	arg1	uptake					1468:1473	water uptake	1462:1473	water uptake	1462:1473	There were 41.63% decreases in water uptake in PVA/N-starch-grafted-poly (EMA) (3:7) composite films as compared to PVA/N-starch composite films.
30452995	0	79	theme	PVA/Trapa	50:58	arg1	films					91:95	PVA/Trapa natans starch (N-st) composite films	50:95	PVA/Trapa natans starch (N-st) composite films	50:95	Studies on the properties and biodegradability of PVA/Trapa natans starch (N-st) composite films and PVA/N-st-g-poly (EMA) composite films.
30452995	7	80	from	increase	1062:1069	arg1	ratio					1078:1082	the ratio	1074:1082	the ratio of grafted starch from (50% to 70%) Elongation at break	1074:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	7	80	from	increase	1062:1069	arg1	break					1134:1138	break	1134:1138	break	1134:1138	With further increase in the ratio of grafted starch from (50% to 70%) Elongation at break, % increased to a great extent.
30452995	3	81	theme	X-ray	586:590	arg1	diffraction					592:602	X-ray diffraction	586:602	X-ray diffraction	586:602	The graft copolymers thus formed were characterized by Fourier transform infrared, scanning electron microscopy, X-ray diffraction and TGA/DTA/DTG techniques.
30452995	2	82	theme	reaction	358:365	arg1	temperature					367:377	reaction temperature	358:377	reaction temperature	358:377	Different reaction parameters such as reaction temperature, time, monomer concentration, pH and solvent were optimized to get maximum graft yield (56%).
31422517	9	0	theme	amino	1608:1612	arg1	similarity					1628:1637	the amino acid sequence similarity	1604:1637	the amino acid sequence similarity between the homologous gene products and MUM2	1604:1683	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	10	1	theme	seed	1907:1910	arg1	mucilage					1912:1919	seed mucilage	1907:1919	seed mucilage	1907:1919	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	3	2	theme	expansion	552:560	arg1	properties					538:547	its unique properties	527:547	its unique properties of expansion, extrusion, and adherence	527:586	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	6	3	theme	promoters	961:969	arg1	ability					931:937	the ability	927:937	the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant	927:1193	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	9	4	theme	sequence	1619:1626	arg1	similarity					1628:1637	the amino acid sequence similarity	1604:1637	the amino acid sequence similarity between the homologous gene products and MUM2	1604:1683	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	6	5	from	ability	931:937	arg1	PEROXIDASE36					1013:1024	PEROXIDASE36	1013:1024	PEROXIDASE36 (PER36)	1013:1032	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	5	from	ability	931:937	arg1	TESTA-ABUNDANT2					989:1003	TESTA-ABUNDANT2	989:1003	TESTA-ABUNDANT2 (TBA2)	989:1010	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	5	from	ability	931:937	arg1	MUCILAGE-MODIFIED4					1039:1056	MUCILAGE-MODIFIED4	1039:1056	MUCILAGE-MODIFIED4 (MUM4)	1039:1063	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	5	from	ability	931:937	arg1	genes					982:986	three genes	976:986	three genes	976:986	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	10	6	theme	seed	1724:1727	arg1	promoters					1743:1751	all three seed coat-specific promoters	1714:1751	all three seed coat-specific promoters	1714:1751	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	5	7	theme	other	835:839	arg1	types					846:850	altering other cell types	826:850	altering other cell types	826:850	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	6	8	theme	seed	942:945	arg1	promoters					961:969	seed coat-specific promoters	942:969	seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4),	942:1064	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	4	9	theme	cell	701:704	arg1	polysaccharides					711:725	manipulating cell wall polysaccharides	688:725	manipulating cell wall polysaccharides	688:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	10	10	theme	negative	1937:1944	arg1	consequences					1946:1957	obvious negative consequences	1929:1957	obvious negative consequences to the rest of the plant	1929:1982	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	2	11	theme	seed	314:317	arg1	cells					334:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	9	12	theme	homologous	1651:1660	arg1	products					1667:1674	the homologous gene products	1647:1674	the homologous gene products	1647:1674	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	4	13	theme	polysaccharides	711:725	arg1	consequences					672:683	the biochemical and biological consequences	641:683	the biochemical and biological consequences of manipulating cell wall polysaccharides	641:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	8	14	used	used	1419:1422	arg2	strongest					1369:1377	strongest	1369:1377	strongest	1369:1377	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	1	15	theme	coat-specific	203:215	arg1	promoters					217:225	three seed coat-specific promoters	192:225	three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes	192:290	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	4	16	theme	biological	661:670	arg1	consequences					672:683	the biochemical and biological consequences	641:683	the biochemical and biological consequences of manipulating cell wall polysaccharides	641:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	7	17	theme	mutant	1347:1352	arg1	phenotype					1354:1362	the mum2 mutant phenotype	1338:1362	the mum2 mutant phenotype	1338:1362	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	2	18	theme	Arabidopsis	293:303	arg1	cells					334:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	1	19	theme	seed	146:149	arg1	mucilage					151:158	seed mucilage	146:158	seed mucilage	146:158	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	0	20	theme	engineer	57:64	arg1	composition					91:101	engineer cell wall polysaccharide composition	57:101	engineer cell wall polysaccharide composition of mucilage	57:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	8	21	theme	composition	1517:1527	arg1	phenotypes					1529:1538	the mum2 extrusion and cell wall composition phenotypes	1484:1538	the mum2 extrusion and cell wall composition phenotypes	1484:1538	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	4	22	theme	cell	741:744	arg1	enzymes					761:767	cell wall-modifying enzymes	741:767	cell wall-modifying enzymes	741:767	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	0	23	theme	wall	71:74	arg1	composition					91:101	engineer cell wall polysaccharide composition	57:101	engineer cell wall polysaccharide composition of mucilage	57:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	10	24	from	expression	1763:1772	arg1	pattern					1846:1852	a spatial and temporal pattern	1823:1852	a spatial and temporal pattern	1823:1852	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	8	25	theme	homologs	1461:1468	arg1	ability					1439:1445	the ability	1435:1445	the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes	1435:1538	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	7	26	theme	promoters	1222:1230	arg1	strength					1200:1207	The strength	1196:1207	The strength of the three promoters relative to one another	1196:1254	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	2	27	theme	epidermal	324:332	arg1	cells					334:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	0	28	theme	mucilage	106:113	arg1	composition					91:101	engineer cell wall polysaccharide composition	57:101	engineer cell wall polysaccharide composition of mucilage	57:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	0	29	theme	coat-specific	30:42	arg1	promoters					44:52	seed coat-specific promoters	25:52	seed coat-specific promoters	25:52	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	8	30	theme	mum2	1488:1491	arg1	extrusion					1493:1501	mum2 extrusion	1488:1501	mum2 extrusion	1488:1501	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	6	31	theme	cell	1081:1084	arg1	wall					1086:1089	the cell wall	1077:1089	the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2)	1077:1162	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	8	32	theme	wall	1512:1515	arg1	composition					1517:1527	cell wall composition	1507:1527	cell wall composition	1507:1527	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	0	33	theme	seed	25:28	arg1	promoters					44:52	seed coat-specific promoters	25:52	seed coat-specific promoters	25:52	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	9	34	theme	complementation	1555:1569	arg1	variable					1575:1582	variable	1575:1582	variable	1575:1582	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	9	34	theme	complementation	1555:1569	arg1	degree					1545:1550	The degree	1541:1550	The degree of complementation	1541:1569	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	3	35	theme	unique	531:536	arg1	properties					538:547	its unique properties	527:547	its unique properties of expansion, extrusion, and adherence	527:586	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	5	36	theme	seed	853:856	arg1	promoters					872:880	seed coat-specific promoters	853:880	seed coat-specific promoters	853:880	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	3	37	dep	composition	481:491	arg1	The					477:479	The	477:479	The	477:479	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	10	38	theme	polysaccharide	1877:1890	arg1	composition					1892:1902	polysaccharide composition	1877:1902	polysaccharide composition	1877:1902	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	1	39	theme	genes	249:253	arg1	expression					235:244	expression	235:244	expression of genes encoding cell wall-modifying enzymes	235:290	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	6	40	theme	gene	1133:1136	arg1	MUCILAGE-MODIFIED2					1138:1155	β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2	1101:1155	β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2)	1101:1162	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	40	theme	gene	1133:1136	arg1	MUM2					1158:1161	MUM2	1158:1161	MUM2	1158:1161	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	2	41	theme	cell	417:420	arg1	mucilage					383:390	mucilage	383:390	mucilage	383:390	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	2	41	theme	cell	417:420	arg1	wall					422:425	a specialized secondary cell wall	393:425	a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin	393:474	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	1	42	theme	cell	264:267	arg1	enzymes					284:290	cell wall-modifying enzymes	264:290	cell wall-modifying enzymes	264:290	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	6	43	from	genes	982:986	arg1	ability					931:937	the ability	927:937	the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant	927:1193	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	43	from	genes	982:986	arg1	promoters					961:969	seed coat-specific promoters	942:969	seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4),	942:1064	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	5	44	theme	altering	826:833	arg1	types					846:850	altering other cell types	826:850	altering other cell types	826:850	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	10	45	theme	obvious	1929:1935	arg1	consequences					1946:1957	obvious negative consequences	1929:1957	obvious negative consequences to the rest of the plant	1929:1982	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	2	46	theme	specialized	395:405	arg1	mucilage					383:390	mucilage	383:390	mucilage	383:390	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	2	46	theme	specialized	395:405	arg1	wall					422:425	a specialized secondary cell wall	393:425	a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin	393:474	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	9	47	theme	acid	1614:1617	arg1	similarity					1628:1637	the amino acid sequence similarity	1604:1637	the amino acid sequence similarity between the homologous gene products and MUM2	1604:1683	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	3	48	theme	adherence	578:586	arg1	properties					538:547	its unique properties	527:547	its unique properties of expansion, extrusion, and adherence	527:586	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	6	49	theme	coat-specific	947:959	arg1	promoters					961:969	seed coat-specific promoters	942:969	seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4),	942:1064	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	2	50	theme	coat	319:322	arg1	cells					334:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells	293:338	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	0	51	theme	promoters	44:52	arg1	utility					14:20	the utility	10:20	the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage	10:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	6	52	theme	mum2	1183:1186	arg1	mutant					1188:1193	the mum2 mutant	1179:1193	the mum2 mutant	1179:1193	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	4	53	theme	manipulating	688:699	arg1	polysaccharides					711:725	manipulating cell wall polysaccharides	688:725	manipulating cell wall polysaccharides	688:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	10	54	theme	coat-specific	1729:1741	arg1	promoters					1743:1751	all three seed coat-specific promoters	1714:1751	all three seed coat-specific promoters	1714:1751	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	7	55	theme	relative	1232:1239	arg1	promoters					1222:1230	the three promoters	1212:1230	the three promoters relative to one another	1212:1254	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	2	56	theme	large	363:367	arg1	quantities					369:378	large quantities	363:378	large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin	363:474	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	1	57	theme	seed	198:201	arg1	promoters					217:225	three seed coat-specific promoters	192:225	three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes	192:290	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	9	58	theme	gene	1662:1665	arg1	products					1667:1674	the homologous gene products	1647:1674	the homologous gene products	1647:1674	The degree of complementation was variable and correlated with the amino acid sequence similarity between the homologous gene products and MUM2.
31422517	2	59	dep	Arabidopsis	293:303	arg1	thaliana					305:312	thaliana	305:312	thaliana	305:312	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	10	60	theme	carbohydrate-active	1792:1810	arg1	enzymes					1812:1818	carbohydrate-active enzymes	1792:1818	carbohydrate-active enzymes	1792:1818	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	4	61	theme	wall	706:709	arg1	polysaccharides					711:725	manipulating cell wall polysaccharides	688:725	manipulating cell wall polysaccharides	688:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	10	62	theme	genes	1777:1781	arg1	expression					1763:1772	expression	1763:1772	expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern	1763:1852	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	1	63	theme	Polysaccharide	116:129	arg1	composition					131:141	Polysaccharide composition	116:141	Polysaccharide composition of seed mucilage	116:158	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	4	64	theme	wall-modifying	746:759	arg1	enzymes					761:767	cell wall-modifying enzymes	741:767	cell wall-modifying enzymes	741:767	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	10	65	theme	plant	1978:1982	arg1	rest					1966:1969	the rest	1962:1969	the rest of the plant	1962:1982	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	10	66	theme	spatial	1825:1831	arg1	pattern					1846:1852	a spatial and temporal pattern	1823:1852	a spatial and temporal pattern	1823:1852	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	7	67	theme	mum2	1342:1345	arg1	phenotype					1354:1362	the mum2 mutant phenotype	1338:1362	the mum2 mutant phenotype	1338:1362	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	0	68	theme	cell	66:69	arg1	composition					91:101	engineer cell wall polysaccharide composition	57:101	engineer cell wall polysaccharide composition of mucilage	57:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	1	69	theme	mucilage	151:158	arg1	composition					131:141	Polysaccharide composition	116:141	Polysaccharide composition of seed mucilage	116:158	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
31422517	8	70	theme	MUM2	1456:1459	arg1	homologs					1461:1468	three MUM2 homologs	1450:1468	three MUM2 homologs	1450:1468	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	0	71	theme	polysaccharide	76:89	arg1	composition					91:101	engineer cell wall polysaccharide composition	57:101	engineer cell wall polysaccharide composition of mucilage	57:113	Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.
31422517	5	72	theme	mucilage	795:802	arg1	composition					804:814	mucilage composition	795:814	mucilage composition	795:814	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	8	73	theme	extrusion	1493:1501	arg1	phenotypes					1529:1538	the mum2 extrusion and cell wall composition phenotypes	1484:1538	the mum2 extrusion and cell wall composition phenotypes	1484:1538	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	5	74	theme	coat-specific	858:870	arg1	promoters					872:880	seed coat-specific promoters	853:880	seed coat-specific promoters	853:880	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	8	75	theme	cell	1507:1510	arg1	composition					1517:1527	cell wall composition	1507:1527	cell wall composition	1507:1527	The strongest of the three promoters, TBA2p, was then used to examine the ability of three MUM2 homologs to complement the mum2 extrusion and cell wall composition phenotypes.
31422517	4	76	theme	seed	607:610	arg1	mucilage					612:619	seed mucilage	607:619	seed mucilage	607:619	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	4	76	theme	seed	607:610	arg1	model					626:630	a model	624:630	a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes	624:767	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	3	77	theme	mucilage	510:517	arg1	structure					497:505	structure	497:505	structure	497:505	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	3	77	theme	mucilage	510:517	arg1	composition					481:491	composition	481:491	composition	481:491	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	5	78	theme	cell	841:844	arg1	types					846:850	altering other cell types	826:850	altering other cell types	826:850	To specifically engineer mucilage composition and avoid altering other cell types, seed coat-specific promoters are required.
31422517	7	79	dep	one	1244:1246	arg1	another					1248:1254	another	1248:1254	another	1248:1254	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	10	80	theme	temporal	1837:1844	arg1	pattern					1846:1852	a spatial and temporal pattern	1823:1852	a spatial and temporal pattern	1823:1852	These data demonstrate that all three seed coat-specific promoters can drive expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern sufficiently to modify polysaccharide composition in seed mucilage without obvious negative consequences to the rest of the plant.
31422517	2	81	theme	mucilage	383:390	arg1	quantities					369:378	large quantities	363:378	large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin	363:474	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	7	82	dep	250	1284:1286	arg1	to					1281:1282	to	1281:1282	to	1281:1282	The strength of the three promoters relative to one another was found to vary by two to 250 fold, and correlated with their ability to rescue the mum2 mutant phenotype.
31422517	2	83	theme	secondary	407:415	arg1	mucilage					383:390	mucilage	383:390	mucilage	383:390	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	2	83	theme	secondary	407:415	arg1	wall					422:425	a specialized secondary cell wall	393:425	a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin	393:474	Arabidopsis thaliana seed coat epidermal cells synthesize and secrete large quantities of mucilage, a specialized secondary cell wall composed of cellulose, hemicellulose, and pectin.
31422517	6	84	theme	-encoding	1123:1131	arg1	MUCILAGE-MODIFIED2					1138:1155	β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2	1101:1155	β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2)	1101:1162	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	6	84	theme	-encoding	1123:1131	arg1	MUM2					1158:1161	MUM2	1158:1161	MUM2	1158:1161	In this study, we investigated the ability of seed coat-specific promoters from three genes, TESTA-ABUNDANT2 (TBA2), PEROXIDASE36 (PER36), and MUCILAGE-MODIFIED4 (MUM4), to express the cell wall modifying β-galactosidase (BGAL)-encoding gene MUCILAGE-MODIFIED2 (MUM2) and complement the mum2 mutant.
31422517	4	85	theme	biochemical	645:655	arg1	consequences					672:683	the biochemical and biological consequences	641:683	the biochemical and biological consequences of manipulating cell wall polysaccharides	641:725	We are developing seed mucilage as a model to study the biochemical and biological consequences of manipulating cell wall polysaccharides in vivo using cell wall-modifying enzymes.
31422517	3	86	theme	extrusion	563:571	arg1	properties					538:547	its unique properties	527:547	its unique properties of expansion, extrusion, and adherence	527:586	The composition and structure of mucilage confers its unique properties of expansion, extrusion, and adherence.
31422517	1	87	theme	wall-modifying	269:282	arg1	enzymes					284:290	cell wall-modifying enzymes	264:290	cell wall-modifying enzymes	264:290	Polysaccharide composition of seed mucilage was successfully modified using three seed coat-specific promoters driving expression of genes encoding cell wall-modifying enzymes.
29525146	0	0	theme	phytosomes	109:118	arg1	delivery					60:67	transmucosal delivery	47:67	transmucosal delivery of novel protamine-decorated tripterine phytosomes	47:118	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	1	1	theme	current	193:199	arg1	study					201:205	the current study	189:205	the current study	189:205	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	2	theme	composite	269:277	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	2	theme	composite	269:277	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	0	3	theme	tripterine	98:107	arg1	phytosomes					109:118	novel protamine-decorated tripterine phytosomes	72:118	novel protamine-decorated tripterine phytosomes	72:118	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	8	4	theme	in-vivo	1313:1319	arg1	study					1337:1341	in-vivo pharmacokinetic study	1313:1341	in-vivo pharmacokinetic study in healthy rabbits	1313:1360	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	7	5	theme	chicken	1139:1145	arg1	mucosa					1153:1158	chicken pouch mucosa	1139:1158	chicken pouch mucosa	1139:1158	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	9	6	theme	novel	1545:1549	arg1	PRT-TRI-PHY					1580:1590	PRT-TRI-PHY	1580:1590	PRT-TRI-PHY	1580:1590	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	9	6	theme	novel	1545:1549	arg1	nanocarrier					1567:1577	a novel mucopenetrating nanocarrier	1543:1577	a novel mucopenetrating nanocarrier	1543:1577	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	0	7	theme	Ex-vivo	121:127	arg1	mucopenetration					129:143	Ex-vivo mucopenetration	121:143	Ex-vivo mucopenetration	121:143	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	1	8	theme	novel	385:389	arg1	PRT-TRI-PHY					424:434	PRT-TRI-PHY	424:434	PRT-TRI-PHY	424:434	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	8	theme	novel	385:389	arg1	layer					417:421	novel mucopenetrating protamine layer	385:421	novel mucopenetrating protamine layer (PRT-TRI-PHY)	385:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	3	9	theme	clinical	571:578	arg1	use					580:582	clinical use	571:582	clinical use of TRI	571:589	However, clinical use of TRI is handicapped by its low oral bioavailability.
29525146	9	10	theme	mucopenetrating	1551:1565	arg1	PRT-TRI-PHY					1580:1590	PRT-TRI-PHY	1580:1590	PRT-TRI-PHY	1580:1590	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	9	10	theme	mucopenetrating	1551:1565	arg1	nanocarrier					1567:1577	a novel mucopenetrating nanocarrier	1543:1577	a novel mucopenetrating nanocarrier	1543:1577	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	2	11	theme	cancers	553:559	arg1	types					544:548	various types	536:548	various types of cancers	536:559	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	1	12	theme	mucopenetrating	391:405	arg1	PRT-TRI-PHY					424:434	PRT-TRI-PHY	424:434	PRT-TRI-PHY	424:434	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	12	theme	mucopenetrating	391:405	arg1	layer					417:421	novel mucopenetrating protamine layer	385:421	novel mucopenetrating protamine layer (PRT-TRI-PHY)	385:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	9	13	theme	prime	1686:1690	arg1	importance					1692:1701	prime importance	1686:1701	prime importance	1686:1701	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	7	14	theme	flux	1236:1239	arg1	value					1241:1245	2.3-folds higher flux value	1219:1245	2.3-folds higher flux value	1219:1245	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	8	15	theme	relative	1454:1461	arg1	bioavailability					1463:1477	relative bioavailability	1454:1477	relative bioavailability of 244%	1454:1485	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	9	16	theme	buccal	1654:1659	arg1	mucosa					1661:1666	buccal mucosa	1654:1666	buccal mucosa which would be of prime importance for its clinical utility	1654:1726	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	1	17	theme	protamine	407:415	arg1	PRT-TRI-PHY					424:434	PRT-TRI-PHY	424:434	PRT-TRI-PHY	424:434	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	17	theme	protamine	407:415	arg1	layer					417:421	novel mucopenetrating protamine layer	385:421	novel mucopenetrating protamine layer (PRT-TRI-PHY)	385:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	4	18	theme	assembly	809:816	arg1	process					818:824	electrostatic assembly process	795:824	electrostatic assembly process	795:824	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	5	19	theme	positive	893:900	arg1	+21.6 mV					918:925	+21.6 mV	918:925	+21.6 mV	918:925	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	5	19	theme	positive	893:900	arg1	potential					907:915	positive zeta potential	893:915	positive zeta potential (+21.6 mV)	893:926	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	7	20	theme	uncoated	1281:1288	arg1	TRI-PHY					1290:1296	uncoated TRI-PHY	1281:1296	uncoated TRI-PHY	1281:1296	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	2	21	theme	potential	497:505	arg1	candidate					518:526	a potential anticancer candidate	495:526	a potential anticancer candidate against various types of cancers	495:559	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	7	22	theme	pouch	1147:1151	arg1	mucosa					1153:1158	chicken pouch mucosa	1139:1158	chicken pouch mucosa	1139:1158	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	4	23	theme	electrostatic	795:807	arg1	process					818:824	electrostatic assembly process	795:824	electrostatic assembly process	795:824	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	1	24	theme	laminated	208:216	arg1	chitosan					218:225	laminated chitosan	208:225	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	24	theme	laminated	208:216	arg1	CS					228:229	CS	228:229	CS	228:229	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	0	25	theme	chitosan-based	10:23	arg1	sponges					35:41	Laminated chitosan-based composite sponges	0:41	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.	0:184	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	2	26	theme	anticancer	507:516	arg1	candidate					518:526	a potential anticancer candidate	495:526	a potential anticancer candidate against various types of cancers	495:559	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	2	27	theme	various	536:542	arg1	types					544:548	various types	536:548	various types of cancers	536:559	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	1	28	theme	chitosan	218:225	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	28	theme	chitosan	218:225	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	0	29	theme	Laminated	0:8	arg1	sponges					35:41	Laminated chitosan-based composite sponges	0:41	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.	0:184	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	5	30	theme	zeta	902:905	arg1	+21.6 mV					918:925	+21.6 mV	918:925	+21.6 mV	918:925	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	5	30	theme	zeta	902:905	arg1	potential					907:915	positive zeta potential	893:915	positive zeta potential (+21.6 mV)	893:926	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	8	31	theme	%	1485:1485	arg1	bioavailability					1463:1477	relative bioavailability	1454:1477	relative bioavailability of 244%	1454:1485	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	8	32	theme	healthy	1346:1352	arg1	rabbits					1354:1360	healthy rabbits	1346:1360	healthy rabbits	1346:1360	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	2	33	theme	herbal	460:465	arg1	drug					467:470	a herbal drug	458:470	a herbal drug widely investigated as a potential anticancer candidate against various types of cancers	458:559	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	2	33	theme	herbal	460:465	arg1	Tripterine					438:447	Tripterine	438:447	Tripterine (TRI)	438:453	Tripterine (TRI) is a herbal drug widely investigated as a potential anticancer candidate against various types of cancers.
29525146	1	34	theme	solid	305:309	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	34	theme	solid	305:309	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	0	35	theme	pharmacokinetic	157:171	arg1	assessments					173:183	in-vivo pharmacokinetic assessments	149:183	in-vivo pharmacokinetic assessments	149:183	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	4	36	theme	protamine	775:783	arg1	layer					785:789	a protamine layer	773:789	a protamine layer via electrostatic assembly process	773:824	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	4	37	theme	pharmaceutical	655:668	arg1	obstacles					670:678	TRI pharmaceutical obstacles	651:678	TRI pharmaceutical obstacles	651:678	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	0	38	theme	composite	25:33	arg1	sponges					35:41	Laminated chitosan-based composite sponges	0:41	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.	0:184	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	6	39	theme	superior	1007:1014	arg1	characteristics					1029:1043	superior mucoadhesion characteristics	1007:1043	superior mucoadhesion characteristics	1007:1043	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	0	40	theme	in-vivo	149:155	arg1	assessments					173:183	in-vivo pharmacokinetic assessments	149:183	in-vivo pharmacokinetic assessments	149:183	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	3	41	theme	TRI	587:589	arg1	use					580:582	clinical use	571:582	clinical use of TRI	571:589	However, clinical use of TRI is handicapped by its low oral bioavailability.
29525146	0	42	theme	transmucosal	47:58	arg1	delivery					60:67	transmucosal delivery	47:67	transmucosal delivery of novel protamine-decorated tripterine phytosomes	47:118	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	9	43	theme	tripterine	1639:1648	arg1	absorption					1625:1634	the absorption	1621:1634	the absorption of tripterine	1621:1648	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	8	44	theme	higher	1389:1394	arg1	bioavailability					1396:1410	the significantly higher bioavailability	1371:1410	the significantly higher bioavailability of PRT-TRI-PHY	1371:1425	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	3	45	theme	low	613:615	arg1	bioavailability					622:636	its low oral bioavailability	609:636	its low oral bioavailability	609:636	However, clinical use of TRI is handicapped by its low oral bioavailability.
29525146	5	46	theme	250 nm	882:887	arg1	size					874:877	a nano-metric size	860:877	a nano-metric size of 250 nm	860:887	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	5	46	theme	250 nm	882:887	arg1	potential					907:915	positive zeta potential	893:915	positive zeta potential (+21.6 mV)	893:926	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	5	46	theme	250 nm	882:887	arg1	+21.6 mV					918:925	+21.6 mV	918:925	+21.6 mV	918:925	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	1	47	theme	buccal	324:329	arg1	delivery					331:338	buccal delivery	324:338	buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY)	324:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	9	48	theme	mucoadhesive	1502:1513	arg1	sponges					1523:1529	mucoadhesive CS-HPMC sponges	1502:1529	mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY,	1502:1591	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	3	49	theme	oral	617:620	arg1	bioavailability					622:636	its low oral bioavailability	609:636	its low oral bioavailability	609:636	However, clinical use of TRI is handicapped by its low oral bioavailability.
29525146	1	50	theme	hydroxypropyl	232:244	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	50	theme	hydroxypropyl	232:244	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	8	51	theme	pharmacokinetic	1321:1335	arg1	study					1337:1341	in-vivo pharmacokinetic study	1313:1341	in-vivo pharmacokinetic study in healthy rabbits	1313:1360	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	6	52	theme	release	986:992	arg1	profile					994:1000	a sustained release profile	974:1000	a sustained release profile with superior mucoadhesion characteristics	974:1043	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	7	53	theme	ex-vivo	1110:1116	arg1	study					1129:1133	The ex-vivo permeation study	1106:1133	The ex-vivo permeation study via chicken pouch mucosa	1106:1158	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	6	54	theme	mucoadhesion	1016:1027	arg1	characteristics					1029:1043	superior mucoadhesion characteristics	1007:1043	superior mucoadhesion characteristics	1007:1043	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	4	55	theme	evaporation	734:744	arg1	technique					746:754	solvent evaporation technique	726:754	solvent evaporation technique then coated with a protamine layer via electrostatic assembly process	726:824	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	5	56	theme	developed	831:839	arg1	PRT-TRI-PHY					841:851	The developed PRT-TRI-PHY	827:851	The developed PRT-TRI-PHY	827:851	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	6	57	theme	sustained	976:984	arg1	profile					994:1000	a sustained release profile	974:1000	a sustained release profile with superior mucoadhesion characteristics	974:1043	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	7	58	theme	permeation	1118:1127	arg1	study					1129:1133	The ex-vivo permeation study	1106:1133	The ex-vivo permeation study via chicken pouch mucosa	1106:1158	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	4	59	theme	solvent	726:732	arg1	technique					746:754	solvent evaporation technique	726:754	solvent evaporation technique then coated with a protamine layer via electrostatic assembly process	726:824	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	1	60	theme	tripterine	343:352	arg1	phytosomes					354:363	tripterine phytosomes	343:363	tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY)	343:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	7	61	theme	higher	1229:1234	arg1	value					1241:1245	2.3-folds higher flux value	1219:1245	2.3-folds higher flux value	1219:1245	The ex-vivo permeation study via chicken pouch mucosa revealed that sponges loaded with PRT-TRI-PHY demonstrated 2.3-folds higher flux value compared with sponges loaded with uncoated TRI-PHY.
29525146	0	62	dep	sponges	35:41	arg1	assessments					173:183	in-vivo pharmacokinetic assessments	149:183	in-vivo pharmacokinetic assessments	149:183	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	0	62	dep	sponges	35:41	arg1	mucopenetration					129:143	Ex-vivo mucopenetration	121:143	Ex-vivo mucopenetration	121:143	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	8	63	from	study	1337:1341	arg1	rabbits					1354:1360	healthy rabbits	1346:1360	healthy rabbits	1346:1360	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	9	64	theme	clinical	1711:1718	arg1	utility					1720:1726	its clinical utility	1707:1726	its clinical utility	1707:1726	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29525146	6	65	with	profile	994:1000	arg1	characteristics					1029:1043	superior mucoadhesion characteristics	1007:1043	superior mucoadhesion characteristics	1007:1043	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	1	66	theme	methylcellulose	246:260	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	66	theme	methylcellulose	246:260	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	8	67	with	TRI-PHY	1441:1447	arg1	bioavailability					1463:1477	relative bioavailability	1454:1477	relative bioavailability of 244%	1454:1485	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	8	68	theme	PRT-TRI-PHY	1415:1425	arg1	bioavailability					1396:1410	the significantly higher bioavailability	1371:1410	the significantly higher bioavailability of PRT-TRI-PHY	1371:1425	Additionally, in-vivo pharmacokinetic study in healthy rabbits revealed the significantly higher bioavailability of PRT-TRI-PHY compared with TRI-PHY with relative bioavailability of 244%.
29525146	6	69	theme	uncoated	1088:1095	arg1	TRI-PHY					1097:1103	uncoated TRI-PHY	1088:1103	uncoated TRI-PHY	1088:1103	Sponges loaded with PRT-TRI-PHY demonstrated a sustained release profile with superior mucoadhesion characteristics compared with the counterparts loaded with uncoated TRI-PHY.
29525146	0	70	theme	protamine-decorated	78:96	arg1	phytosomes					109:118	novel protamine-decorated tripterine phytosomes	72:118	novel protamine-decorated tripterine phytosomes	72:118	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	4	71	theme	TRI	681:683	arg1	phytosomes					685:694	TRI phytosomes	681:694	TRI phytosomes (TRI-PHY)	681:704	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	4	71	theme	TRI	681:683	arg1	TRI-PHY					697:703	TRI-PHY	697:703	TRI-PHY	697:703	To surmount TRI pharmaceutical obstacles, TRI phytosomes (TRI-PHY) were prepared using solvent evaporation technique then coated with a protamine layer via electrostatic assembly process.
29525146	1	72	theme	HPMC	263:266	arg1	matrices					311:318	solid matrices	305:318	solid matrices	305:318	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	1	72	theme	HPMC	263:266	arg1	sponges					279:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges	208:285	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	0	73	theme	novel	72:76	arg1	phytosomes					109:118	novel protamine-decorated tripterine phytosomes	72:118	novel protamine-decorated tripterine phytosomes	72:118	Laminated chitosan-based composite sponges for transmucosal delivery of novel protamine-decorated tripterine phytosomes: Ex-vivo mucopenetration and in-vivo pharmacokinetic assessments.
29525146	1	74	theme	phytosomes	354:363	arg1	delivery					331:338	buccal delivery	324:338	buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY)	324:435	In the current study, laminated chitosan (CS):hydroxypropyl methylcellulose (HPMC) composite sponges were exploited as solid matrices for buccal delivery of tripterine phytosomes functionalized with novel mucopenetrating protamine layer (PRT-TRI-PHY).
29525146	5	75	theme	nano-metric	862:872	arg1	size					874:877	a nano-metric size	860:877	a nano-metric size of 250 nm	860:887	The developed PRT-TRI-PHY showed a nano-metric size of 250 nm and positive zeta potential (+21.6 mV).
29525146	9	76	theme	CS-HPMC	1515:1521	arg1	sponges					1523:1529	mucoadhesive CS-HPMC sponges	1502:1529	mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY,	1502:1591	Conclusively, mucoadhesive CS-HPMC sponges loaded with a novel mucopenetrating nanocarrier, PRT-TRI-PHY, could significantly improve the absorption of tripterine via buccal mucosa which would be of prime importance for its clinical utility.
29663923	4	0	theme	cancer	798:803	arg1	therapy					805:811	magnetically targeted cancer therapy	776:811	magnetically targeted cancer therapy	776:811	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	13	1	theme	cell	1729:1732	arg1	viability					1734:1742	breast cancer cell viability	1715:1742	breast cancer cell viability	1715:1742	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	11	2	theme	mV	1536:1537	arg1	potential					1517:1525	a zeta potential	1510:1525	a zeta potential of 29.59 mV	1510:1537	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	11	2	theme	mV	1536:1537	arg1	properties					1552:1561	magnetic properties	1543:1561	magnetic properties of 35.932 emu/g	1543:1577	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	3	3	theme	interest	457:464	arg1	Selenium					418:425	Selenium	418:425	Selenium	418:425	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	3	3	theme	interest	457:464	arg1	trace					432:436	a trace mineral	430:444	a trace mineral of growing interest	430:464	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	8	4	theme	zeta	1074:1077	arg1	potential					1079:1087	zeta potential	1074:1087	zeta potential	1074:1087	The particles were characterized for size, shape, zeta potential and magnetic properties.
29663923	11	5	theme	emu/g	1573:1577	arg1	potential					1517:1525	a zeta potential	1510:1525	a zeta potential of 29.59 mV	1510:1537	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	11	5	theme	emu/g	1573:1577	arg1	properties					1552:1561	magnetic properties	1543:1561	magnetic properties of 35.932 emu/g	1543:1577	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	13	6	theme	incubation	1703:1712	arg1	day					1696:1698	just 1 day	1689:1698	just 1 day of incubation	1689:1712	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	12	7	theme	cancer	1642:1647	arg1	properties					1657:1666	concentration dependent cancer killing properties	1618:1666	concentration dependent cancer killing properties	1618:1666	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	7	8	theme	coating	1007:1013	arg1	methods					1015:1021	different chitosan and selenium coating methods	975:1021	different chitosan and selenium coating methods	975:1021	This was followed by different chitosan and selenium coating methods.
29663923	10	9	theme	oxide	1338:1342	arg1	core					1344:1347	an iron oxide core	1330:1347	an iron oxide core produced by thermal decomposition	1330:1381	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	15	10	theme	anticancer	2241:2250	arg1	applications					2252:2263	magnetically targeted anticancer applications	2219:2263	magnetically targeted anticancer applications	2219:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	3	11	theme	essential	501:509	arg1	it					492:493	it	492:493	it	492:493	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	3	11	theme	essential	501:509	arg1	nutrient					511:518	an essential nutrient	498:518	an essential nutrient in the human body	498:536	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	7	12	theme	different	975:983	arg1	methods					1015:1021	different chitosan and selenium coating methods	975:1021	different chitosan and selenium coating methods	975:1021	This was followed by different chitosan and selenium coating methods.
29663923	1	13	theme	magnetic	217:224	arg1	MRI					245:247	MRI	245:247	MRI	245:247	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	1	13	theme	magnetic	217:224	arg1	imaging					236:242	magnetic resonance imaging	217:242	magnetic resonance imaging (MRI)	217:248	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	9	14	theme	cancer	1167:1172	arg1	properties					1182:1191	cancer killing properties	1167:1191	cancer killing properties on MB-231 breast cancer cells	1167:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	3	15	theme	human	527:531	arg1	body					533:536	the human body	523:536	the human body	523:536	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	5	16	theme	IONP	842:845	arg1	design					828:833	The suggested design	814:833	The suggested design	814:833	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	5	16	theme	IONP	842:845	arg1	core					847:850	an IONP core	839:850	an IONP core	839:850	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	3	17	theme	thiolcontaining	561:575	arg1	proteins					577:584	thiolcontaining proteins	561:584	thiolcontaining proteins necessary for cancer cells to function	561:623	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	15	18	theme	new	2102:2104	arg1	nanoparticle					2116:2127	a brand new composite nanoparticle	2094:2127	a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications	2094:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	12	19	theme	dependent	1632:1640	arg1	properties					1657:1666	concentration dependent cancer killing properties	1618:1666	concentration dependent cancer killing properties	1618:1666	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	11	20	from	nm	1489:1490	arg1	diameter					1495:1502	diameter	1495:1502	diameter	1495:1502	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	13	21	theme	nanoparticles	1811:1823	arg1	μg/ml					1786:1790	1 μg/ml	1784:1790	1 μg/ml of these composite nanoparticles	1784:1823	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	9	22	theme	MB-231	1196:1201	arg1	cells					1217:1221	MB-231 breast cancer cells	1196:1221	MB-231 breast cancer cells	1196:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	5	23	theme	suggested	818:826	arg1	design					828:833	The suggested design	814:833	The suggested design	814:833	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	5	23	theme	suggested	818:826	arg1	core					847:850	an IONP core	839:850	an IONP core	839:850	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	6	24	theme	IONP	928:931	arg1	cores					933:937	IONP cores	928:937	IONP cores	928:937	Two different types of IONP cores were produced.
29663923	9	25	theme	cancer	1210:1215	arg1	cells					1217:1221	MB-231 breast cancer cells	1196:1221	MB-231 breast cancer cells	1196:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	1	26	from	field	167:171	arg1	well-established					135:150	well-established	135:150	well-established	135:150	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	12	27	theme	novel	1594:1598	arg1	nanoparticles					1600:1612	the novel nanoparticles	1590:1612	the novel nanoparticles	1590:1612	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	10	28	theme	thermal	1361:1367	arg1	decomposition					1369:1381	thermal decomposition	1361:1381	thermal decomposition	1361:1381	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	5	29	dep	core	847:850	arg1	stabilized					853:862	stabilized	853:862	stabilized by chitosan	853:874	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	5	29	dep	core	847:850	arg1	decorated					880:888	decorated	880:888	decorated with selenium	880:902	The suggested design was an IONP core, stabilized by chitosan and decorated with selenium.
29663923	10	30	theme	silane	1398:1403	arg1	coating					1433:1439	a chitosan coating	1422:1439	a chitosan coating	1422:1439	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	30	theme	silane	1398:1403	arg1	decoration					1454:1463	selenium decoration	1445:1463	selenium decoration	1445:1463	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	30	theme	silane	1398:1403	arg1	exchange					1412:1419	a silane ligand exchange	1396:1419	a silane ligand exchange	1396:1419	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	13	31	theme	composite	1801:1809	arg1	nanoparticles					1811:1823	these composite nanoparticles	1795:1823	these composite nanoparticles	1795:1823	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	2	32	theme	lower	370:374	arg1	concentrations					376:389	lower concentrations	370:389	lower concentrations that IONP (e.g., 1 μg/ml)	370:415	Moreover, selenium has been shown to kill numerous cancer cells at lower concentrations that IONP (e.g., 1 μg/ml).
29663923	1	33	from	well-established	135:150	arg1	field					167:171	the medical field	155:171	the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI)	155:248	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	1	34	theme	oxide	96:100	arg1	IONP					117:120	IONP	117:120	IONP	117:120	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	1	34	theme	oxide	96:100	arg1	nanoparticles					102:114	Iron oxide nanoparticles	91:114	Iron oxide nanoparticles (IONP)	91:121	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	14	35	theme	pharmaceutical	1987:2000	arg1	drugs					2002:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	4	36	theme	in	666:667	arg1	study					675:679	this in vitro study	661:679	this in vitro study	661:679	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	1	37	theme	external	264:271	arg1	control					282:288	their external magnetic control	258:288	their external magnetic control in the body	258:300	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	4	38	theme	study	675:679	arg1	goal					653:656	the goal	649:656	the goal of this in vitro study	649:679	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	0	39	theme	Iron	13:16	arg1	Oxide					18:22	Iron Oxide	13:22	Iron Oxide	13:22	Selenium and Iron Oxide Nanocomposites for Magnetically-Targeted Anti-Cancer Applications.
29663923	14	40	used	used	2050:2053	arg2	magnetism					2035:2043	magnetism	2035:2043	magnetism	2035:2043	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	14	40	used	used	2050:2053	arg2	stimulation					2018:2028	infrared stimulation	2009:2028	infrared stimulation	2009:2028	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	14	40	used	used	2050:2053	arg2	drugs					2002:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	3	41	theme	cancer	600:605	arg1	cells					607:611	cancer cells	600:611	cancer cells	600:611	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	10	42	theme	selenium	1445:1452	arg1	decoration					1454:1463	selenium decoration	1445:1463	selenium decoration	1445:1463	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	42	theme	selenium	1445:1452	arg1	exchange					1412:1419	a silane ligand exchange	1396:1419	a silane ligand exchange	1396:1419	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	3	43	theme	necessary	586:594	arg1	proteins					577:584	thiolcontaining proteins	561:584	thiolcontaining proteins necessary for cancer cells to function	561:623	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	13	44	theme	chemotherapeutic	1888:1903	arg1	drug					1920:1923	a chemotherapeutic pharmaceutical drug	1886:1923	a chemotherapeutic pharmaceutical drug	1886:1923	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	3	45	theme	mineral	438:444	arg1	Selenium					418:425	Selenium	418:425	Selenium	418:425	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	3	45	theme	mineral	438:444	arg1	trace					432:436	a trace mineral	430:444	a trace mineral of growing interest	430:464	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	0	46	theme	Magnetically-Targeted	43:63	arg1	Applications					77:88	Magnetically-Targeted Anti-Cancer Applications	43:88	Magnetically-Targeted Anti-Cancer Applications	43:88	Selenium and Iron Oxide Nanocomposites for Magnetically-Targeted Anti-Cancer Applications.
29663923	10	47	theme	pioneering	1240:1249	arg1	study					1251:1255	this pioneering study	1235:1255	this pioneering study	1235:1255	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	1	48	theme	resonance	226:234	arg1	MRI					245:247	MRI	245:247	MRI	245:247	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	1	48	theme	resonance	226:234	arg1	imaging					236:242	magnetic resonance imaging	217:242	magnetic resonance imaging (MRI)	217:248	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	13	49	dep	reduced	1748:1754	arg1	reduced					1844:1850	reduced	1844:1850	reduced at even 0.1 μg/ml	1844:1868	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	1	50	theme	medical	159:165	arg1	field					167:171	the medical field	155:171	the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI)	155:248	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	12	51	contain	had	1614:1616	arg1	nanoparticles					1600:1612	the novel nanoparticles	1590:1612	the novel nanoparticles	1590:1612	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	12	51	contain	had	1614:1616	arg2	properties					1657:1666	concentration dependent cancer killing properties	1618:1666	concentration dependent cancer killing properties	1618:1666	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	10	52	theme	promising	1278:1286	arg1	nanocomposites					1302:1315	the most promising iron-selenium nanocomposites	1269:1315	the most promising iron-selenium nanocomposites	1269:1315	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	2	53	theme	numerous	345:352	arg1	cells					361:365	numerous cancer cells	345:365	numerous cancer cells	345:365	Moreover, selenium has been shown to kill numerous cancer cells at lower concentrations that IONP (e.g., 1 μg/ml).
29663923	13	54	theme	cancer	1722:1727	arg1	viability					1734:1742	breast cancer cell viability	1715:1742	breast cancer cell viability	1715:1742	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	3	55	theme	growing	449:455	arg1	interest					457:464	growing interest	449:464	growing interest	449:464	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	4	56	theme	targeted	789:796	arg1	therapy					805:811	magnetically targeted cancer therapy	776:811	magnetically targeted cancer therapy	776:811	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	13	57	dep	μg/ml	1786:1790	arg1	presence					1772:1779	presence	1772:1779	presence	1772:1779	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	13	57	dep	μg/ml	1786:1790	arg1	the					1768:1770	the	1768:1770	the	1768:1770	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	1	58	from	control	282:288	arg1	body					297:300	the body	293:300	the body	293:300	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	15	59	theme	brand	2096:2100	arg1	nanoparticle					2116:2127	a brand new composite nanoparticle	2094:2127	a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications	2094:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	15	60	dep	iron	2143:2146	arg1	selenium					2158:2165	selenium	2158:2165	selenium	2158:2165	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	15	60	dep	iron	2143:2146	arg1	oxide					2148:2152	oxide	2148:2152	oxide	2148:2152	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	4	61	theme	composite	748:756	arg1	nano-vehicles					758:770	composite nano-vehicles	748:770	composite nano-vehicles for magnetically targeted cancer therapy	748:811	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	12	62	theme	killing	1649:1655	arg1	properties					1657:1666	concentration dependent cancer killing properties	1618:1666	concentration dependent cancer killing properties	1618:1666	Moreover, the novel nanoparticles had concentration dependent cancer killing properties.
29663923	7	63	theme	selenium	998:1005	arg1	methods					1015:1021	different chitosan and selenium coating methods	975:1021	different chitosan and selenium coating methods	975:1021	This was followed by different chitosan and selenium coating methods.
29663923	3	64	from	trace	432:436	arg1	treatment					476:484	cancer treatment	469:484	cancer treatment	469:484	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	8	65	theme	magnetic	1093:1100	arg1	properties					1102:1111	magnetic properties	1093:1111	magnetic properties	1093:1111	The particles were characterized for size, shape, zeta potential and magnetic properties.
29663923	14	66	theme	significant	1936:1946	arg1	finding					1948:1954	a significant finding	1934:1954	a significant finding	1934:1954	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	14	66	theme	significant	1936:1946	arg1	This					1926:1929	This	1926:1929	This	1926:1929	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	9	67	theme	most	1127:1130	arg1	products					1142:1149	the most promising products	1123:1149	the most promising products	1123:1149	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	7	68	theme	chitosan	985:992	arg1	methods					1015:1021	different chitosan and selenium coating methods	975:1021	different chitosan and selenium coating methods	975:1021	This was followed by different chitosan and selenium coating methods.
29663923	9	69	theme	promising	1132:1140	arg1	products					1142:1149	the most promising products	1123:1149	the most promising products	1123:1149	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	13	70	theme	pharmaceutical	1905:1918	arg1	drug					1920:1923	a chemotherapeutic pharmaceutical drug	1886:1923	a chemotherapeutic pharmaceutical drug	1886:1923	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	15	71	theme	composite	2106:2114	arg1	nanoparticle					2116:2127	a brand new composite nanoparticle	2094:2127	a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications	2094:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	10	72	theme	iron	1333:1336	arg1	core					1344:1347	an iron oxide core	1330:1347	an iron oxide core produced by thermal decomposition	1330:1381	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	9	73	theme	killing	1174:1180	arg1	properties					1182:1191	cancer killing properties	1167:1191	cancer killing properties on MB-231 breast cancer cells	1167:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	4	74	dep	in	666:667	arg1	vitro					669:673	vitro	669:673	vitro	669:673	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	3	75	from	nutrient	511:518	arg1	body					533:536	the human body	523:536	the human body	523:536	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	15	76	theme	targeted	2232:2239	arg1	applications					2252:2263	magnetically targeted anticancer applications	2219:2263	magnetically targeted anticancer applications	2219:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	6	77	theme	cores	933:937	arg1	types					919:923	Two different types	905:923	Two different types of IONP cores	905:937	Two different types of IONP cores were produced.
29663923	9	78	theme	breast	1203:1208	arg1	cells					1217:1221	MB-231 breast cancer cells	1196:1221	MB-231 breast cancer cells	1196:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	13	79	dep	reduced	1844:1850	arg1	statistically					1830:1842	statistically	1830:1842	statistically	1830:1842	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	4	80	theme	above	634:638	arg1	reasons					640:646	the above reasons	630:646	the above reasons	630:646	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	6	81	theme	different	909:917	arg1	types					919:923	Two different types	905:923	Two different types of IONP cores	905:937	Two different types of IONP cores were produced.
29663923	14	82	theme	infrared	2009:2016	arg1	stimulation					2018:2028	infrared stimulation	2009:2028	infrared stimulation	2009:2028	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	1	83	theme	Iron	91:94	arg1	oxide					96:100	Iron oxide	91:100	Iron oxide nanoparticles (IONP)	91:121	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	2	84	dep	μg/ml	410:414	arg1	e.g.					402:405	e.g.	402:405	e.g.	402:405	Moreover, selenium has been shown to kill numerous cancer cells at lower concentrations that IONP (e.g., 1 μg/ml).
29663923	14	85	theme	chemotherapeutic	1970:1985	arg1	drugs					2002:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	neither chemotherapeutic pharmaceutical drugs	1962:2006	This is a significant finding since neither chemotherapeutic pharmaceutical drugs, infrared stimulation, nor magnetism were used.
29663923	2	86	theme	cancer	354:359	arg1	cells					361:365	numerous cancer cells	345:365	numerous cancer cells	345:365	Moreover, selenium has been shown to kill numerous cancer cells at lower concentrations that IONP (e.g., 1 μg/ml).
29663923	1	87	theme	magnetic	273:280	arg1	control					282:288	their external magnetic control	258:288	their external magnetic control in the body	258:300	Iron oxide nanoparticles (IONP) are already well-established in the medical field due to their ability to improve contrast in magnetic resonance imaging (MRI) and for their external magnetic control in the body.
29663923	11	88	with	nm	1489:1490	arg1	potential					1517:1525	a zeta potential	1510:1525	a zeta potential of 29.59 mV	1510:1537	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	11	88	with	nm	1489:1490	arg1	properties					1552:1561	magnetic properties	1543:1561	magnetic properties of 35.932 emu/g	1543:1577	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	10	89	theme	ligand	1405:1410	arg1	coating					1433:1439	a chitosan coating	1422:1439	a chitosan coating	1422:1439	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	89	theme	ligand	1405:1410	arg1	decoration					1454:1463	selenium decoration	1445:1463	selenium decoration	1445:1463	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	89	theme	ligand	1405:1410	arg1	exchange					1412:1419	a silane ligand exchange	1396:1419	a silane ligand exchange	1396:1419	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	4	90	theme	first	726:730	arg1	time					732:735	the first time to develop composite nano-vehicles for magnetically targeted cancer therapy	722:811	the first time to develop composite nano-vehicles for magnetically targeted cancer therapy	722:811	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	0	91	theme	Anti-Cancer	65:75	arg1	Applications					77:88	Magnetically-Targeted Anti-Cancer Applications	43:88	Magnetically-Targeted Anti-Cancer Applications	43:88	Selenium and Iron Oxide Nanocomposites for Magnetically-Targeted Anti-Cancer Applications.
29663923	15	92	theme	applications	2252:2263	arg1	range					2210:2214	a wide range	2203:2214	a wide range of magnetically targeted anticancer applications	2203:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29663923	10	93	theme	study	1251:1255	arg1	Results					1224:1230	Results	1224:1230	Results of this pioneering study	1224:1255	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	94	theme	most	1273:1276	arg1	nanocomposites					1302:1315	the most promising iron-selenium nanocomposites	1269:1315	the most promising iron-selenium nanocomposites	1269:1315	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	95	theme	chitosan	1424:1431	arg1	coating					1433:1439	a chitosan coating	1422:1439	a chitosan coating	1422:1439	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	10	95	theme	chitosan	1424:1431	arg1	exchange					1412:1419	a silane ligand exchange	1396:1419	a silane ligand exchange	1396:1419	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	4	96	theme	above	700:704	arg1	chemistries					706:716	the above chemistries	696:716	the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy	696:811	For the above reasons, the goal of this in vitro study was to combine the above chemistries for the first time to develop composite nano-vehicles for magnetically targeted cancer therapy.
29663923	10	97	theme	iron-selenium	1288:1300	arg1	nanocomposites					1302:1315	the most promising iron-selenium nanocomposites	1269:1315	the most promising iron-selenium nanocomposites	1269:1315	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	9	98	from	properties	1182:1191	arg1	cells					1217:1221	MB-231 breast cancer cells	1196:1221	MB-231 breast cancer cells	1196:1221	Finally, the most promising products were tested for cancer killing properties on MB-231 breast cancer cells.
29663923	13	99	theme	breast	1715:1720	arg1	cancer					1722:1727	breast cancer	1715:1727	breast cancer cell viability	1715:1742	Specifically, after just 1 day of incubation, breast cancer cell viability was reduced to 40.5% in the presence of 1 μg/ml of these composite nanoparticles (and statistically reduced at even 0.1 μg/ml), without using a chemotherapeutic pharmaceutical drug.
29663923	11	100	theme	magnetic	1543:1550	arg1	properties					1552:1561	magnetic properties	1543:1561	magnetic properties of 35.932 emu/g	1543:1577	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	11	101	theme	zeta	1512:1515	arg1	potential					1517:1525	a zeta potential	1510:1525	a zeta potential of 29.59 mV	1510:1537	The particles were 5-9 nm in diameter, with a zeta potential of 29.59 mV and magnetic properties of 35.932 emu/g.
29663923	3	102	theme	cancer	469:474	arg1	treatment					476:484	cancer treatment	469:484	cancer treatment	469:484	Selenium is a trace mineral of growing interest in cancer treatment since it is an essential nutrient in the human body and can interfere with thiolcontaining proteins necessary for cancer cells to function.
29663923	10	103	dep	consisted	1317:1325	arg1	followed					1384:1391	followed	1384:1391	followed by a silane ligand exchange, a chitosan coating and selenium decoration	1384:1463	Results of this pioneering study showed that the most promising iron-selenium nanocomposites consisted of an iron oxide core produced by thermal decomposition, followed by a silane ligand exchange, a chitosan coating and selenium decoration.
29663923	15	104	theme	wide	2205:2208	arg1	range					2210:2214	a wide range	2203:2214	a wide range of magnetically targeted anticancer applications	2203:2263	In this manner, this study introduces a brand new composite nanoparticle consisting of iron oxide and selenium which should be further studied for a wide range of magnetically targeted anticancer applications.
29722535	0	0	theme	Selenite	95:102	arg1	Remediation					104:114	Selenite Remediation	95:114	Selenite Remediation	95:114	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	6	1	theme	joint	772:776	arg1	effect					778:783	the joint effect	768:783	the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix	768:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	7	2	theme	FeS	965:967	arg1	effect					955:960	the oxidation retardation effect	929:960	the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions	929:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	9	3	theme	electron	1200:1207	arg1	balance					1209:1215	electron balance	1200:1215	electron balance	1200:1215	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	6	4	with	comparable	752:761	arg1	effect					778:783	the joint effect	768:783	the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix	768:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	8	5	theme	regenerated	1036:1046	arg1	FeS-SA					1048:1053	The biogenic regenerated FeS-SA	1023:1053	The biogenic regenerated FeS-SA	1023:1053	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	8	6	theme	Fe	1131:1132	arg1	leaching					1134:1141	the Fe leaching	1127:1141	the Fe leaching	1127:1141	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	8	7	theme	40	1068:1069	arg1	%					1070:1070	%	1070:1070	%	1070:1070	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	6	8	theme	homogeneous	832:842	arg1	distribution					844:855	the homogeneous distribution	828:855	the homogeneous distribution of FeS in SA matrix	828:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	1	9	theme	sulfide	122:128	arg1	nanoparticles					136:148	Iron sulfide (FeS) nanoparticles	117:148	Iron sulfide (FeS) nanoparticles	117:148	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	4	10	theme	pure	505:508	arg1	FeS					524:526	pure nonstabilized FeS	505:526	pure nonstabilized FeS	505:526	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	3	11	theme	immobilization	322:335	arg1	technology					337:346	in situ immobilization technology	314:346	in situ immobilization technology	314:346	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	9	12	theme	metal	1240:1244	arg1	species					1278:1284	the main selenium species	1260:1284	the main selenium species	1260:1284	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	12	theme	metal	1240:1244	arg1	selenium					1246:1253	metal selenium	1240:1253	metal selenium	1240:1253	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	12	theme	metal	1240:1244	arg1	FeSe					1231:1234	FeSe	1231:1234	FeSe	1231:1234	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	7	13	theme	oxidation	933:941	arg1	retardation					943:953	oxidation retardation	933:953	the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions	929:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	10	14	theme	excellent	1362:1370	arg1	prospect					1384:1391	an excellent application prospect	1359:1391	an excellent application prospect	1359:1391	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	1	15	theme	selenite	172:179	arg1	remediation					190:200	selenite (Se(IV)) remediation	172:200	selenite (Se(IV)) remediation in recent decades	172:218	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	0	16	theme	Sulfide	39:45	arg1	Composite					81:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	3	17	theme	nanoparticle-impregnated	375:398	arg1	FeS-SA					420:425	FeS-SA	420:425	FeS-SA	420:425	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	3	17	theme	nanoparticle-impregnated	375:398	arg1	composite					409:417	FeS nanoparticle-impregnated alginate composite	371:417	FeS nanoparticle-impregnated alginate composite (FeS-SA)	371:426	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	4	18	theme	nonstabilized	510:522	arg1	FeS					524:526	pure nonstabilized FeS	505:526	pure nonstabilized FeS	505:526	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	8	19	theme	%	1070:1070	arg1	efficiency					1080:1089	40% removal efficiency	1068:1089	40% removal efficiency for Se(IV)	1068:1100	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	5	20	theme	pure	660:663	arg1	FeS					676:678	pure stabilized FeS	660:678	pure stabilized FeS	660:678	The removal efficiency increased to 73% when pure stabilized FeS was used.
29722535	6	21	from	distribution	844:855	arg1	matrix					870:875	SA matrix	867:875	SA matrix	867:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	10	22	theme	application	1372:1382	arg1	prospect					1384:1391	an excellent application prospect	1359:1391	an excellent application prospect	1359:1391	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	3	23	dep	in	314:315	arg1	situ					317:320	situ	317:320	situ	317:320	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	0	24	theme	In	0:1	arg1	Preparation					8:18	In Situ Preparation	0:18	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.	0:115	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	8	25	theme	removal	1072:1078	arg1	efficiency					1080:1089	40% removal efficiency	1068:1089	40% removal efficiency for Se(IV)	1068:1100	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	9	26	theme	reference	1176:1184	arg1	compounds					1186:1194	the reference compounds	1172:1194	the reference compounds	1172:1194	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	27	theme	main	1264:1267	arg1	species					1278:1284	the main selenium species	1260:1284	the main selenium species	1260:1284	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	27	theme	main	1264:1267	arg1	selenium					1246:1253	metal selenium	1240:1253	metal selenium	1240:1253	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	27	theme	main	1264:1267	arg1	FeSe					1231:1234	FeSe	1231:1234	FeSe	1231:1234	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	7	28	theme	anoxic	995:1000	arg1	conditions					1011:1020	anoxic and oxic conditions	995:1020	anoxic and oxic conditions	995:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	5	29	theme	stabilized	665:674	arg1	FeS					676:678	pure stabilized FeS	660:678	pure stabilized FeS	660:678	The removal efficiency increased to 73% when pure stabilized FeS was used.
29722535	1	30	theme	Iron	117:120	arg1	FeS					131:133	FeS	131:133	FeS	131:133	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	1	30	theme	Iron	117:120	arg1	sulfide					122:128	Iron sulfide	117:128	Iron sulfide (FeS) nanoparticles	117:148	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	9	31	theme	selenium	1269:1276	arg1	species					1278:1284	the main selenium species	1260:1284	the main selenium species	1260:1284	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	31	theme	selenium	1269:1276	arg1	selenium					1246:1253	metal selenium	1240:1253	metal selenium	1240:1253	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	9	31	theme	selenium	1269:1276	arg1	FeSe					1231:1234	FeSe	1231:1234	FeSe	1231:1234	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	8	32	theme	biogenic	1027:1034	arg1	FeS-SA					1048:1053	The biogenic regenerated FeS-SA	1023:1053	The biogenic regenerated FeS-SA	1023:1053	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	6	33	theme	SA	812:813	arg1	beads					815:819	pure stabilized FeS and SA beads	788:819	pure stabilized FeS and SA beads	788:819	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	10	34	theme	in	1308:1309	arg1	preparation					1316:1326	This in situ preparation	1303:1326	This in situ preparation of stabilized FeS-SA	1303:1347	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	6	35	theme	removal	724:730	arg1	efficiency					732:741	superior removal efficiency	715:741	superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix	715:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	2	36	theme	FeS	270:272	arg1	oxidization					255:265	oxidization	255:265	oxidization	255:265	However, the easy aggregation and oxidization of FeS hamper their reactivity.
29722535	2	36	theme	FeS	270:272	arg1	aggregation					239:249	easy aggregation	234:249	easy aggregation	234:249	However, the easy aggregation and oxidization of FeS hamper their reactivity.
29722535	4	37	theme	alginate	539:546	arg1	beads					553:557	sodium alginate (SA) beads	532:557	sodium alginate (SA) beads	532:557	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	0	38	theme	Iron	34:37	arg1	Composite					81:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	7	39	theme	minor	891:895	arg1	differences					897:907	minor differences	891:907	minor differences	891:907	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	6	40	theme	superior	715:722	arg1	efficiency					732:741	superior removal efficiency	715:741	superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix	715:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	10	41	theme	Se	1415:1416	arg1	remediation					1400:1410	the remediation	1396:1410	the remediation of Se(IV)	1396:1420	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	9	42	theme	XPS	1144:1146	arg1	technique					1148:1156	XPS technique	1144:1156	XPS technique combined with the reference compounds and electron balance	1144:1215	XPS technique combined with the reference compounds and electron balance revealed that FeSe and metal selenium were the main selenium species after treatment.
29722535	0	43	theme	Stabilized	23:32	arg1	Composite					81:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	4	44	theme	Se	479:480	arg1	IV					482:483	IV	482:483	IV	482:483	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	4	44	theme	Se	479:480	arg1	%					470:470	100%	467:470	100% of the Se(IV) (0.13 mM)	467:494	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	4	44	theme	Se	479:480	arg1	Se					479:480	the Se	475:480	the Se(IV) (0.13 mM)	475:494	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	4	44	theme	Se	479:480	arg1	mM					492:493	0.13 mM	487:493	0.13 mM	487:493	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	3	45	theme	alginate	400:407	arg1	FeS-SA					420:425	FeS-SA	420:425	FeS-SA	420:425	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	3	45	theme	alginate	400:407	arg1	composite					409:417	FeS nanoparticle-impregnated alginate composite	371:417	FeS nanoparticle-impregnated alginate composite (FeS-SA)	371:426	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	6	46	theme	FeS	860:862	arg1	distribution					844:855	the homogeneous distribution	828:855	the homogeneous distribution of FeS in SA matrix	828:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	3	47	theme	Se	432:433	arg1	remediation					439:449	Se(IV) remediation	432:449	Se(IV) remediation	432:449	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	0	48	theme	Nanoparticle-Impregnated	47:70	arg1	Composite					81:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	6	49	theme	FeS	804:806	arg1	beads					815:819	pure stabilized FeS and SA beads	788:819	pure stabilized FeS and SA beads	788:819	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	10	50	theme	FeS-SA	1342:1347	arg1	preparation					1316:1326	This in situ preparation	1303:1326	This in situ preparation of stabilized FeS-SA	1303:1347	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	5	51	theme	removal	619:625	arg1	efficiency					627:636	The removal efficiency	615:636	The removal efficiency	615:636	The removal efficiency increased to 73% when pure stabilized FeS was used.
29722535	1	52	from	remediation	190:200	arg1	decades					212:218	recent decades	205:218	recent decades	205:218	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	4	53	theme	Se	593:594	arg1	%					584:584	only 27 and 20%	570:584	only 27 and 20% of the Se(IV)	570:598	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	4	53	theme	Se	593:594	arg1	Se					593:594	the Se	589:594	the Se(IV)	589:598	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	4	53	theme	Se	593:594	arg1	IV					596:597	IV	596:597	IV	596:597	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	3	54	theme	FeS	371:373	arg1	FeS-SA					420:425	FeS-SA	420:425	FeS-SA	420:425	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	3	54	theme	FeS	371:373	arg1	composite					409:417	FeS nanoparticle-impregnated alginate composite	371:417	FeS nanoparticle-impregnated alginate composite (FeS-SA)	371:426	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	6	55	theme	pure	788:791	arg1	beads					815:819	pure stabilized FeS and SA beads	788:819	pure stabilized FeS and SA beads	788:819	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	3	56	theme	in	314:315	arg1	technology					337:346	in situ immobilization technology	314:346	in situ immobilization technology	314:346	In this study, in situ immobilization technology was applied to prepare FeS nanoparticle-impregnated alginate composite (FeS-SA) for Se(IV) remediation.
29722535	7	57	theme	retardation	943:953	arg1	effect					955:960	the oxidation retardation effect	929:960	the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions	929:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	4	58	dep	removed	459:465	arg1	whereas					497:503	whereas	497:503	whereas	497:503	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	7	59	theme	SA	980:981	arg1	beads					983:987	SA beads	980:987	SA beads under anoxic and oxic conditions	980:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	2	60	theme	easy	234:237	arg1	aggregation					239:249	easy aggregation	234:249	easy aggregation	234:249	However, the easy aggregation and oxidization of FeS hamper their reactivity.
29722535	8	61	theme	due	1120:1122	arg1	cycles					1113:1118	five cycles	1108:1118	five cycles due to the Fe leaching	1108:1141	The biogenic regenerated FeS-SA still showed 40% removal efficiency for Se(IV) after five cycles due to the Fe leaching.
29722535	6	62	theme	SA	867:868	arg1	matrix					870:875	SA matrix	867:875	SA matrix	867:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	5	63	used	used	684:687	arg2	FeS					676:678	pure stabilized FeS	660:678	pure stabilized FeS	660:678	The removal efficiency increased to 73% when pure stabilized FeS was used.
29722535	7	64	theme	oxic	1006:1009	arg1	conditions					1011:1020	anoxic and oxic conditions	995:1020	anoxic and oxic conditions	995:1020	Furthermore, minor differences were established in the oxidation retardation effect of FeS exerted by SA beads under anoxic and oxic conditions.
29722535	6	65	theme	beads	815:819	arg1	effect					778:783	the joint effect	768:783	the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix	768:875	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
29722535	1	66	theme	recent	205:210	arg1	decades					212:218	recent decades	205:218	recent decades	205:218	Iron sulfide (FeS) nanoparticles have been applied for selenite (Se(IV)) remediation in recent decades.
29722535	0	67	theme	Composite	81:89	arg1	Preparation					8:18	In Situ Preparation	0:18	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.	0:115	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	4	68	theme	SA	549:550	arg1	beads					553:557	sodium alginate (SA) beads	532:557	sodium alginate (SA) beads	532:557	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	10	69	dep	in	1308:1309	arg1	situ					1311:1314	situ	1311:1314	situ	1311:1314	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	10	70	theme	stabilized	1331:1340	arg1	FeS-SA					1342:1347	stabilized FeS-SA	1331:1347	stabilized FeS-SA	1331:1347	This in situ preparation of stabilized FeS-SA exhibited an excellent application prospect in the remediation of Se(IV).
29722535	4	71	theme	sodium	532:537	arg1	beads					553:557	sodium alginate (SA) beads	532:557	sodium alginate (SA) beads	532:557	FeS-SA removed 100% of the Se(IV) (0.13 mM), whereas pure nonstabilized FeS and sodium alginate (SA) beads eliminated only 27 and 20% of the Se(IV), respectively.
29722535	0	72	theme	Alginate	72:79	arg1	Composite					81:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite	23:89	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	0	73	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Preparation of Stabilized Iron Sulfide Nanoparticle-Impregnated Alginate Composite for Selenite Remediation.
29722535	2	74	dep	aggregation	239:249	arg1	the					230:232	the	230:232	the	230:232	However, the easy aggregation and oxidization of FeS hamper their reactivity.
29722535	6	75	theme	stabilized	793:802	arg1	beads					815:819	pure stabilized FeS and SA beads	788:819	pure stabilized FeS and SA beads	788:819	Therefore, FeS-SA showed superior removal efficiency that was comparable with the joint effect of pure stabilized FeS and SA beads due to the homogeneous distribution of FeS in SA matrix.
31013093	6	0	theme	composite	1172:1180	arg1	information					1182:1192	composite information	1172:1192	composite information of CD44 expression, HA binding, and internalization	1172:1244	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	6	1	theme	expression	1202:1211	arg1	information					1182:1192	composite information	1172:1192	composite information of CD44 expression, HA binding, and internalization	1172:1244	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	5	2	theme	cancer	1093:1098	arg1	lines					1105:1109	the cancer cell lines	1089:1109	the cancer cell lines	1089:1109	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	10	3	theme	CD44	2068:2071	arg1	profile					2084:2090	their CD44 expression profile	2062:2090	their CD44 expression profile	2062:2090	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	6	4	theme	internalization	1230:1244	arg1	information					1182:1192	composite information	1172:1192	composite information of CD44 expression, HA binding, and internalization	1172:1244	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	1	5	theme	promising	151:159	arg1	strategy					161:168	a promising strategy	149:168	a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies	149:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	5	6	theme	cell	1100:1103	arg1	lines					1105:1109	the cancer cell lines	1089:1109	the cancer cell lines	1089:1109	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	3	7	theme	uptake	666:671	arg1	"					672:672	collectively called "uptake"	645:672	collectively called "uptake"	645:672	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	3	7	theme	uptake	666:671	arg1	internalization					628:642	internalization	628:642	internalization (collectively called "uptake") into CD44-expressing cells	628:700	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	2	8	theme	investigated	285:296	arg1	agents					298:303	the most investigated agents	276:303	the most investigated agents for cancer targeting	276:324	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	2	9	theme	agents	298:303	arg1	agents					298:303	the most investigated agents	276:303	the most investigated agents for cancer targeting	276:324	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	2	9	theme	agents	298:303	arg1	one					269:271	one	269:271	one	269:271	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	4	10	theme	large	869:873	arg1	panel					875:879	a large panel	867:879	a large panel of cancer cell lines	867:900	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	9	11	theme	underlying	1771:1780	arg1	s					1792:1792	the underlying mechanism(s)	1767:1793	the underlying mechanism(s) for HA uptake	1767:1807	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	4	12	theme	cancer	884:889	arg1	lines					896:900	cancer cell lines	884:900	cancer cell lines	884:900	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	3	13	theme	CD44/HA	443:449	arg1	interaction					451:461	CD44/HA interaction	443:461	CD44/HA interaction	443:461	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	10	14	theme	receptor	1870:1877	arg1	amount					1855:1860	the amount	1851:1860	the amount of CD44 receptor	1851:1877	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	10	14	theme	receptor	1870:1877	arg1	receptor					1870:1877	CD44 receptor	1865:1877	CD44 receptor	1865:1877	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	4	15	dep	pancreatic	946:955	arg1	ovarian					958:964	ovarian	958:964	ovarian	958:964	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	16	theme	CD44	738:741	arg1	expression					724:733	the expression	720:733	the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively)	720:812	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	8	17	theme	CD44shigh	1548:1556	arg1	HDFs					1558:1561	CD44shigh HDFs	1548:1561	CD44shigh HDFs	1548:1561	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	9	18	theme	first	1673:1677	arg1	step					1679:1682	the first step	1669:1682	the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake	1669:1807	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	9	18	theme	first	1673:1677	arg1	experiments					1638:1648	The experiments	1634:1648	The experiments described here	1634:1663	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	3	19	from	studies	432:438	arg1	interaction					451:461	CD44/HA interaction	443:461	CD44/HA interaction	443:461	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	7	20	theme	other	1450:1454	arg1	words					1456:1460	other words	1450:1460	other words	1450:1460	In other words, CD44shigh cells bound and internalized more HA compared to CD44slow cells.
31013093	6	21	theme	positive	1382:1389	arg1	correlation					1391:1401	a positive correlation	1380:1401	a positive correlation	1380:1401	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	4	22	dep	standard	749:756	arg1	both					744:747	both	744:747	both	744:747	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	3	23	theme	CD44-expressing	680:694	arg1	cells					696:700	CD44-expressing cells	680:700	CD44-expressing cells	680:700	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	6	24	theme	CD44	1197:1200	arg1	expression					1202:1211	CD44 expression	1197:1211	CD44 expression	1197:1211	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	3	25	theme	modified	518:525	arg1	systems					527:533	cell-free or genetically modified systems	493:533	cell-free or genetically modified systems	493:533	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	6	26	theme	HA	1324:1325	arg1	uptake					1314:1319	the uptake	1310:1319	the uptake of HA	1310:1325	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	2	27	theme	cancer	309:314	arg1	targeting					316:324	cancer targeting	309:324	cancer targeting	309:324	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	4	28	theme	endometrial	992:1002	arg1	cancers					1004:1010	endometrial cancers	992:1010	endometrial cancers	992:1010	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	28	theme	endometrial	992:1002	arg1	fibroblasts					844:854	human dermal fibroblasts	831:854	human dermal fibroblasts (HDFs)	831:861	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	28	theme	endometrial	992:1002	arg1	breast					913:918	breast	913:918	breast	913:918	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	10	29	dep	important	1946:1954	arg1	advance					1956:1962	advance	1956:1962	advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile	1956:2090	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	10	30	theme	tumors	2033:2038	arg1	selection					2020:2028	the selection	2016:2028	the selection of tumors	2016:2038	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	10	30	theme	tumors	2033:2038	arg1	design					1984:1989	the design	1980:1989	the design of HA-based carriers	1980:2010	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	7	31	theme	CD44slow	1522:1529	arg1	cells					1531:1535	CD44slow cells	1522:1535	CD44slow cells	1522:1535	In other words, CD44shigh cells bound and internalized more HA compared to CD44slow cells.
31013093	1	32	theme	related	189:195	arg1	issues					182:187	issues	182:187	issues related to nonspecific effects of conventional anticancer therapies	182:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	2	33	theme	many	397:400	arg1	cancers					402:408	many cancers	397:408	many cancers	397:408	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	6	34	theme	binding	1217:1223	arg1	information					1182:1192	composite information	1172:1192	composite information of CD44 expression, HA binding, and internalization	1172:1244	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	1	35	theme	delivery	94:101	arg1	systems					103:109	delivery systems	94:109	delivery systems	94:109	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	4	36	dep	CD44	738:741	arg1	standard					749:756	standard	749:756	standard	749:756	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	36	dep	CD44	738:741	arg1	CD44v					793:797	CD44v	793:797	CD44v	793:797	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	36	dep	CD44	738:741	arg1	variants					762:769	variants	762:769	variants	762:769	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	36	dep	CD44	738:741	arg1	CD44s					783:787	designated CD44s	772:787	designated CD44s	772:787	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	0	37	theme	Acid	40:43	arg1	Efficiency					15:24	the Efficiency	11:24	the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44	11:72	Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
31013093	6	38	theme	HA	1214:1215	arg1	binding					1217:1223	HA binding	1214:1223	HA binding	1214:1223	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	9	39	theme	mechanism	1782:1790	arg1	s					1792:1792	the underlying mechanism(s)	1767:1793	the underlying mechanism(s) for HA uptake	1767:1807	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	8	40	theme	uptaking	1586:1593	arg1	HA					1595:1596	uptaking HA	1586:1596	uptaking HA	1586:1596	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	1	41	theme	nonspecific	200:210	arg1	effects					212:218	nonspecific effects	200:218	nonspecific effects of conventional anticancer therapies	200:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	0	42	theme	Hyaluronic	29:38	arg1	Acid					40:43	Hyaluronic Acid	29:43	Hyaluronic Acid	29:43	Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
31013093	5	43	theme	isoform	1038:1044	arg1	profiles					1046:1053	CD44 isoform profiles	1033:1053	CD44 isoform profiles	1033:1053	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	10	44	theme	CD44	1865:1868	arg1	receptor					1870:1877	CD44 receptor	1865:1877	CD44 receptor	1865:1877	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	10	45	theme	other	1840:1844	arg1	factors					1832:1838	factors	1832:1838	factors other than the amount of CD44 receptor	1832:1877	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	0	46	theme	Tumor	49:53	arg1	Targeting					55:63	Tumor Targeting	49:63	Tumor Targeting via CD44	49:72	Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
31013093	7	47	theme	CD44shigh	1463:1471	arg1	cells					1473:1477	CD44shigh cells	1463:1477	CD44shigh cells	1463:1477	In other words, CD44shigh cells bound and internalized more HA compared to CD44slow cells.
31013093	4	48	theme	dermal	837:842	arg1	prostate					921:928	prostate	921:928	prostate	921:928	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	cancers					1004:1010	endometrial cancers	992:1010	endometrial cancers	992:1010	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	pancreatic					946:955	pancreatic	946:955	pancreatic	946:955	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	head					931:934	head	931:934	head	931:934	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	fibroblasts					844:854	human dermal fibroblasts	831:854	human dermal fibroblasts (HDFs)	831:861	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	colorectal					967:976	colorectal	967:976	colorectal	967:976	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	breast					913:918	breast	913:918	breast	913:918	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	thyroid					979:985	thyroid	979:985	thyroid	979:985	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	48	theme	dermal	837:842	arg1	HDFs					857:860	HDFs	857:860	HDFs	857:860	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	3	49	theme	HA	613:614	arg1	binding					616:622	HA binding	613:622	HA binding	613:622	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	9	50	theme	HA	1799:1800	arg1	uptake					1802:1807	HA uptake	1799:1807	HA uptake	1799:1807	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	1	51	theme	systems	103:109	arg1	development					79:89	The development	75:89	The development of delivery systems capable of tumor targeting	75:136	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	5	52	theme	CD44	1033:1036	arg1	profiles					1046:1053	CD44 isoform profiles	1033:1053	CD44 isoform profiles	1033:1053	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	6	53	theme	CD44v	1279:1283	arg1	expression					1265:1274	the expression	1261:1274	the expression of CD44v	1261:1283	Using composite information of CD44 expression, HA binding, and internalization, we found that the expression of CD44v can negatively influence the uptake of HA, and, instead, when cells primarily expressed CD44s, a positive correlation was observed between expression and uptake.
31013093	4	54	theme	human	831:835	arg1	prostate					921:928	prostate	921:928	prostate	921:928	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	cancers					1004:1010	endometrial cancers	992:1010	endometrial cancers	992:1010	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	pancreatic					946:955	pancreatic	946:955	pancreatic	946:955	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	head					931:934	head	931:934	head	931:934	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	fibroblasts					844:854	human dermal fibroblasts	831:854	human dermal fibroblasts (HDFs)	831:861	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	colorectal					967:976	colorectal	967:976	colorectal	967:976	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	breast					913:918	breast	913:918	breast	913:918	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	thyroid					979:985	thyroid	979:985	thyroid	979:985	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	54	theme	human	831:835	arg1	HDFs					857:860	HDFs	857:860	HDFs	857:860	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	10	55	theme	expression	2073:2082	arg1	profile					2084:2090	their CD44 expression profile	2062:2090	their CD44 expression profile	2062:2090	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	4	56	theme	designated	772:781	arg1	variants					762:769	variants	762:769	variants	762:769	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	56	theme	designated	772:781	arg1	CD44s					783:787	designated CD44s	772:787	designated CD44s	772:787	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	8	57	theme	cancer	1620:1625	arg1	cells					1627:1631	CD44shigh cancer cells	1610:1631	CD44shigh cancer cells	1610:1631	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	1	58	theme	capable	111:117	arg1	development					79:89	The development	75:89	The development of delivery systems capable of tumor targeting	75:136	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	5	59	theme	expression	1059:1068	arg1	levels					1070:1075	expression levels	1059:1075	expression levels	1059:1075	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	3	60	theme	called	658:663	arg1	"					672:672	collectively called "uptake"	645:672	collectively called "uptake"	645:672	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	3	60	theme	called	658:663	arg1	internalization					628:642	internalization	628:642	internalization (collectively called "uptake") into CD44-expressing cells	628:700	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	3	61	theme	cell-related	582:593	arg1	factors					595:601	cell-related factors	582:601	cell-related factors	582:601	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	8	62	theme	CD44shigh	1610:1618	arg1	cells					1627:1631	CD44shigh cancer cells	1610:1631	CD44shigh cancer cells	1610:1631	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	2	63	theme	hyaluronic	329:338	arg1	acid					340:343	hyaluronic acid	329:343	hyaluronic acid (HA)	329:348	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	2	63	theme	hyaluronic	329:338	arg1	HA					346:347	HA	346:347	HA	346:347	Currently, one of the most investigated agents for cancer targeting is hyaluronic acid (HA), since its receptor, CD44, is overexpressed in many cancers.
31013093	8	64	from	HA	1595:1596	arg1	efficient					1573:1581	efficient	1573:1581	efficient	1573:1581	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	10	65	with	interaction	1902:1912	arg1	HA					1919:1920	HA	1919:1920	HA	1919:1920	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	3	66	theme	cell-free	493:501	arg1	systems					527:533	cell-free or genetically modified systems	493:533	cell-free or genetically modified systems	493:533	However, most of the studies on CD44/HA interaction have been so far performed in cell-free or genetically modified systems, thus leaving some uncertainty regarding which cell-related factors influence HA binding and internalization (collectively called "uptake") into CD44-expressing cells.
31013093	10	67	theme	carriers	2003:2010	arg1	selection					2020:2028	the selection	2016:2028	the selection of tumors	2016:2038	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	10	67	theme	carriers	2003:2010	arg1	design					1984:1989	the design	1980:1989	the design of HA-based carriers	1980:2010	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	1	68	theme	tumor	122:126	arg1	targeting					128:136	tumor targeting	122:136	tumor targeting	122:136	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	1	69	theme	conventional	223:234	arg1	therapies					247:255	conventional anticancer therapies	223:255	conventional anticancer therapies	223:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	4	70	theme	lines	896:900	arg1	prostate					921:928	prostate	921:928	prostate	921:928	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	cancers					1004:1010	endometrial cancers	992:1010	endometrial cancers	992:1010	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	pancreatic					946:955	pancreatic	946:955	pancreatic	946:955	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	head					931:934	head	931:934	head	931:934	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	fibroblasts					844:854	human dermal fibroblasts	831:854	human dermal fibroblasts (HDFs)	831:861	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	panel					875:879	a large panel	867:879	a large panel of cancer cell lines	867:900	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	colorectal					967:976	colorectal	967:976	colorectal	967:976	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	breast					913:918	breast	913:918	breast	913:918	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	thyroid					979:985	thyroid	979:985	thyroid	979:985	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	4	70	theme	lines	896:900	arg1	HDFs					857:860	HDFs	857:860	HDFs	857:860	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	5	71	theme	cell	1153:1156	arg1	origin					1158:1163	the cell origin	1149:1163	the cell origin	1149:1163	Results showed that CD44 isoform profiles and expression levels vary across the cancer cell lines and HDF and are not consistent within the cell origin.
31013093	10	72	theme	HA-based	1994:2001	arg1	carriers					2003:2010	HA-based carriers	1994:2010	HA-based carriers	1994:2010	The results show that factors other than the amount of CD44 receptor can play a role in the interaction with HA, and this represents an important advance with respect to the design of HA-based carriers and the selection of tumors to treat according to their CD44 expression profile.
31013093	1	73	theme	targeting	128:136	arg1	capable					111:117	capable	111:117	capable	111:117	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	9	74	theme	CD44	1727:1730	arg1	expression					1732:1741	CD44 expression	1727:1741	CD44 expression	1727:1741	The experiments described here are the first step toward understanding the interplay between CD44 expression, its functionality, and the underlying mechanism(s) for HA uptake.
31013093	1	75	theme	anticancer	236:245	arg1	therapies					247:255	conventional anticancer therapies	223:255	conventional anticancer therapies	223:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31013093	4	76	theme	cell	891:894	arg1	lines					896:900	cancer cell lines	884:900	cancer cell lines	884:900	To address this, the expression of CD44 (both standard and variants, designated CD44s and CD44v, respectively) was evaluated in human dermal fibroblasts (HDFs) and a large panel of cancer cell lines, including breast, prostate, head and neck, pancreatic, ovarian, colorectal, thyroid, and endometrial cancers.
31013093	8	77	from	efficient	1573:1581	arg1	HA					1595:1596	uptaking HA	1586:1596	uptaking HA	1586:1596	Moreover, CD44shigh HDFs were less efficient in uptaking HA compared to CD44shigh cancer cells.
31013093	1	78	theme	therapies	247:255	arg1	effects					212:218	nonspecific effects	200:218	nonspecific effects of conventional anticancer therapies	200:255	The development of delivery systems capable of tumor targeting represents a promising strategy to overcome issues related to nonspecific effects of conventional anticancer therapies.
31826477	0	0	theme	genus	75:79	arg1	strain					51:56	a novel strain	43:56	a novel strain of Lactobacillus genus	43:79	Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus.
31826477	2	1	theme	pre-column	330:339	arg1	derivatization					341:354	pre-column derivatization	330:354	pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone	330:390	The compositional analysis of BC was carried out by HPLC system using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone showed that it consisted of glucan.
31826477	0	2	theme	Lactobacillus	61:73	arg1	genus					75:79	Lactobacillus genus	61:79	Lactobacillus genus	61:79	Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus.
31826477	4	3	theme	morphological	669:681	arg1	similarities					683:694	the morphological similarities	665:694	the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm	665:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	4	4	with	fibrils	798:804	arg1	width					822:826	an average width	811:826	an average width of 45 nm	811:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	0	5	from	Studies	0:6	arg1	cellulose					21:29	bacterial cellulose	11:29	bacterial cellulose	11:29	Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus.
31826477	4	6	theme	reticulated	751:761	arg1	structure					763:771	a reticulated structure	749:771	a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm	749:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	5	7	theme	7.23 ± 0.59 g L-1	951:967	arg1	BC					969:970	7.23 ± 0.59 g L-1 BC	951:970	7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1)	951:1054	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	1	8	theme	Bacterial	239:247	arg1	cellulose					249:257	Bacterial cellulose	239:257	Bacterial cellulose	239:257	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	4	9	with	similarities	683:694	arg1	nano-cellulose					711:724	bacterial nano-cellulose	701:724	bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm	701:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	2	10	theme	compositional	264:276	arg1	analysis					278:285	The compositional analysis	260:285	The compositional analysis of BC	260:291	The compositional analysis of BC was carried out by HPLC system using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone showed that it consisted of glucan.
31826477	3	11	contain	had	474:476	arg2	nano-cellulose					488:501	bacterial nano-cellulose	478:501	bacterial nano-cellulose	478:501	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	3	11	contain	had	474:476	arg1	it					471:472	it	471:472	it	471:472	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	5	12	theme	conversions	977:987	arg1	sugar					1008:1012	0.30 g BC/g sugar	996:1012	0.30 g BC/g sugar	996:1012	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	12	theme	conversions	977:987	arg1	yield					989:993	conversions yield	977:993	conversions yield (0.30 g BC/g sugar)	977:1013	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	4	13	theme	BC	653:654	arg1	FE-SEM					631:636	FE-SEM	631:636	FE-SEM	631:636	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	4	13	theme	BC	653:654	arg1	TEM					642:644	TEM	642:644	TEM	642:644	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	1	14	theme	bacterium	142:150	arg1	IITRKH159					176:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	4	15	theme	45 nm	831:835	arg1	width					822:826	an average width	811:826	an average width of 45 nm	811:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	4	16	theme	structure	763:771	arg1	presence					737:744	the presence	733:744	the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm	733:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	1	17	theme	Lactobacillus	152:164	arg1	IITRKH159					176:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	5	18	theme	cultivation	1096:1106	arg1	days					1088:1091	16 days	1085:1091	16 days of cultivation	1085:1106	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	1	19	theme	hilgardii	166:174	arg1	IITRKH159					176:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	the bacterium Lactobacillus hilgardii IITRKH159	138:184	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	1	20	theme	IITRKH159	176:184	arg1	attribute					125:133	a novel attribute	117:133	a novel attribute	117:133	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	2	21	theme	BC	290:291	arg1	analysis					278:285	The compositional analysis	260:285	The compositional analysis of BC	260:291	The compositional analysis of BC was carried out by HPLC system using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone showed that it consisted of glucan.
31826477	5	22	theme	BC	969:970	arg1	production					937:946	the production	933:946	the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium	933:1077	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	0	23	theme	bacterial	11:19	arg1	cellulose					21:29	bacterial cellulose	11:29	bacterial cellulose	11:29	Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus.
31826477	3	24	dep	pure	529:532	arg1	crystalline					542:552	crystalline	542:552	crystalline	542:552	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	3	24	dep	pure	529:532	arg1	%					560:560	90.25%	555:560	90.25%	555:560	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	5	25	theme	modified	1061:1068	arg1	Y-medium					1070:1077	a modified Y-medium	1059:1077	a modified Y-medium	1059:1077	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	4	26	theme	average	814:820	arg1	width					822:826	an average width	811:826	an average width of 45 nm	811:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	3	27	theme	Physiochemical	432:445	arg1	study					447:451	The Physiochemical study	428:451	The Physiochemical study of BC	428:457	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	3	28	theme	BC	456:457	arg1	study					447:451	The Physiochemical study	428:451	The Physiochemical study of BC	428:457	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	5	29	theme	carbon	862:867	arg1	sources					869:875	different carbon sources	852:875	different carbon sources	852:875	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	3	30	theme	Iα	595:596	arg1	form					598:601	Iα form	595:601	Iα form of cellulose	595:614	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	0	31	theme	novel	45:49	arg1	strain					51:56	a novel strain	43:56	a novel strain of Lactobacillus genus	43:79	Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus.
31826477	4	32	theme	bacterial	701:709	arg1	nano-cellulose					711:724	bacterial nano-cellulose	701:724	bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm	701:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	1	33	attach	isolated	187:194	arg2	attribute					125:133	a novel attribute	117:133	a novel attribute	117:133	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	1	33	attach	isolated	187:194	arg1	Sapodilla					201:209	Sapodilla	201:209	Sapodilla	201:209	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	5	34	from	production	937:946	arg1	Y-medium					1070:1077	a modified Y-medium	1059:1077	a modified Y-medium	1059:1077	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	4	35	theme	nano-sized	787:796	arg1	fibrils					798:804	nano-sized fibrils	787:804	nano-sized fibrils with an average width of 45 nm	787:835	Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm.
31826477	3	36	theme	cellulose	606:614	arg1	form					598:601	Iα form	595:601	Iα form of cellulose	595:614	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	1	37	theme	present	86:92	arg1	study					94:98	The present study	82:98	The present study	82:98	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	5	38	theme	BC	902:903	arg1	production					905:914	BC production	902:914	BC production	902:914	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	39	theme	0.30 g	996:1001	arg1	sugar					1008:1012	0.30 g BC/g sugar	996:1012	0.30 g BC/g sugar	996:1012	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	39	theme	0.30 g	996:1001	arg1	yield					989:993	conversions yield	977:993	conversions yield (0.30 g BC/g sugar)	977:1013	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	3	40	theme	bacterial	478:486	arg1	nano-cellulose					488:501	bacterial nano-cellulose	478:501	bacterial nano-cellulose	478:501	The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with Iα form of cellulose.
31826477	2	41	with	derivatization	341:354	arg1	1-phenyl-3-methyl-5-pyrazolone					361:390	1-phenyl-3-methyl-5-pyrazolone	361:390	1-phenyl-3-methyl-5-pyrazolone	361:390	The compositional analysis of BC was carried out by HPLC system using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone showed that it consisted of glucan.
31826477	5	42	theme	BC/g	1003:1006	arg1	sugar					1008:1012	0.30 g BC/g sugar	996:1012	0.30 g BC/g sugar	996:1012	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	42	theme	BC/g	1003:1006	arg1	yield					989:993	conversions yield	977:993	conversions yield (0.30 g BC/g sugar)	977:1013	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	1	43	theme	novel	119:123	arg1	attribute					125:133	a novel attribute	117:133	a novel attribute	117:133	The present study aimed to explore a novel attribute of the bacterium Lactobacillus hilgardii IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose.
31826477	5	44	theme	different	852:860	arg1	sources					869:875	different carbon sources	852:875	different carbon sources	852:875	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	45	theme	sources	869:875	arg1	effect					842:847	The effect	838:847	The effect of different carbon sources	838:875	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	46	with	BC	969:970	arg1	sugar					1008:1012	0.30 g BC/g sugar	996:1012	0.30 g BC/g sugar	996:1012	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	46	with	BC	969:970	arg1	productivity					1019:1030	productivity	1019:1030	productivity (0.45 ± 0.15 g L-1 d-1)	1019:1054	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	46	with	BC	969:970	arg1	L-1 d-1					1047:1053	0.45 ± 0.15 g L-1 d-1	1033:1053	0.45 ± 0.15 g L-1 d-1	1033:1053	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31826477	5	46	with	BC	969:970	arg1	yield					989:993	conversions yield	977:993	conversions yield (0.30 g BC/g sugar)	977:1013	The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L-1 BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L-1 d-1) in a modified Y-medium after 16 days of cultivation.
31884080	7	0	theme	angle	953:957	arg1	analysis					959:966	the water contact angle analysis	935:966	the water contact angle analysis	935:966	The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis.
31884080	11	1	theme	pure	1354:1357	arg1	nanofibers					1363:1372	pure CAB nanofibers	1354:1372	pure CAB nanofibers	1354:1372	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	3	2	from	application	402:412	arg1	engineering					424:434	tissue engineering	417:434	tissue engineering	417:434	However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion.
31884080	6	3	theme	composite	779:787	arg1	nanofibers					789:798	CAB/PEG composite nanofibers	771:798	CAB/PEG composite nanofibers	771:798	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	12	4	theme	tissue	1495:1500	arg1	engineering					1502:1512	tissue engineering	1495:1512	tissue engineering	1495:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	5	5	theme	2:1	687:689	arg1	ratio					678:682	only the ratio	669:682	only the ratio of 2:1	669:689	Different ratios of CAB to PEG were tested and only the ratio of 2:1 resulted in smooth and bead-free nanofibers.
31884080	5	6	theme	CAB	642:644	arg1	ratios					632:637	Different ratios	622:637	Different ratios of CAB to PEG	622:651	Different ratios of CAB to PEG were tested and only the ratio of 2:1 resulted in smooth and bead-free nanofibers.
31884080	6	7	theme	CAB/PEG	771:777	arg1	nanofibers					789:798	CAB/PEG composite nanofibers	771:798	CAB/PEG composite nanofibers	771:798	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	0	8	theme	potential	93:101	arg1	scaffold					103:110	A potential scaffold	91:110	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	8	9	theme	swelling	1006:1013	arg1	ability					1015:1021	the swelling ability	1002:1021	the swelling ability of the composite nanofiber	1002:1048	As the hydrophilicity increases, the swelling ability of the composite nanofiber increases by 2-fold with more rapid biodegradation.
31884080	9	10	theme	dermal	1170:1175	arg1	NHDF					1190:1193	NHDF	1190:1193	NHDF	1190:1193	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	9	10	theme	dermal	1170:1175	arg1	fibroblasts					1177:1187	normal human dermal fibroblasts	1157:1187	normal human dermal fibroblasts (NHDF)	1157:1194	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	11	11	theme	CAB	1359:1361	arg1	nanofibers					1363:1372	pure CAB nanofibers	1354:1372	pure CAB nanofibers	1354:1372	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	4	12	theme	CAB	575:577	arg1	PEG					616:618	PEG	616:618	PEG	616:618	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	4	12	theme	CAB	575:577	arg1	glycol					608:613	CAB and hydrophilic polyethylene glycol	575:613	CAB and hydrophilic polyethylene glycol (PEG)	575:619	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	0	13	theme	tissue	116:121	arg1	engineering					123:133	tissue engineering	116:133	tissue engineering	116:133	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	11	14	theme	cell	1326:1329	arg1	attachment					1331:1340	better cell attachment	1319:1340	better cell attachment compared to pure CAB nanofibers	1319:1372	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	12	15	theme	composite	1404:1412	arg1	scaffold					1466:1473	a nanofiber scaffold	1454:1473	a nanofiber scaffold for applications in tissue engineering	1454:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	12	15	theme	composite	1404:1412	arg1	nanofibers					1414:1423	CAB/PEG composite nanofibers	1396:1423	CAB/PEG composite nanofibers	1396:1423	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	3	16	theme	CAB	387:389	arg1	hydrophobicity					369:382	the intrinsic hydrophobicity	355:382	the intrinsic hydrophobicity of CAB	355:389	However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion.
31884080	1	17	theme	nanofiber	182:190	arg1	scaffolds					192:200	nanofiber scaffolds	182:200	nanofiber scaffolds	182:200	Electrospinning is a common method to prepare nanofiber scaffolds for tissue engineering.
31884080	0	18	theme	cellulose	12:20	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	6	19	theme	CAB	846:848	arg1	nanofibers					850:859	pure CAB nanofibers	841:859	pure CAB nanofibers	841:859	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	8	20	theme	nanofiber	1040:1048	arg1	ability					1015:1021	the swelling ability	1002:1021	the swelling ability of the composite nanofiber	1002:1048	As the hydrophilicity increases, the swelling ability of the composite nanofiber increases by 2-fold with more rapid biodegradation.
31884080	6	21	theme	test	748:751	arg1	results					753:759	The tensile test results	736:759	The tensile test results	736:759	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	3	22	theme	tissue	417:422	arg1	engineering					424:434	tissue engineering	417:434	tissue engineering	417:434	However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion.
31884080	0	23	theme	Electrospun	0:10	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	9	24	theme	human	1164:1168	arg1	NHDF					1190:1193	NHDF	1190:1193	NHDF	1190:1193	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	9	24	theme	human	1164:1168	arg1	fibroblasts					1177:1187	normal human dermal fibroblasts	1157:1187	normal human dermal fibroblasts (NHDF)	1157:1194	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	0	25	theme	butyrate/polyethylene	30:50	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	6	26	theme	tensile	740:746	arg1	results					753:759	The tensile test results	736:759	The tensile test results	736:759	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	12	27	from	applications	1479:1490	arg1	engineering					1502:1512	tissue engineering	1495:1512	tissue engineering	1495:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	3	28	theme	intrinsic	359:367	arg1	hydrophobicity					369:382	the intrinsic hydrophobicity	355:382	the intrinsic hydrophobicity of CAB	355:389	However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion.
31884080	7	29	theme	nanofibers	898:907	arg1	hydrophobicity					866:879	The hydrophobicity	862:879	The hydrophobicity of the composite nanofibers	862:907	The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis.
31884080	0	30	dep	nanofibers	79:88	arg1	scaffold					103:110	A potential scaffold	91:110	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	9	31	theme	normal	1157:1162	arg1	NHDF					1190:1193	NHDF	1190:1193	NHDF	1190:1193	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	9	31	theme	normal	1157:1162	arg1	fibroblasts					1177:1187	normal human dermal fibroblasts	1157:1187	normal human dermal fibroblasts (NHDF)	1157:1194	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	1	32	theme	tissue	206:211	arg1	engineering					213:223	tissue engineering	206:223	tissue engineering	206:223	Electrospinning is a common method to prepare nanofiber scaffolds for tissue engineering.
31884080	0	33	theme	acetate	22:28	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	6	34	theme	pure	841:844	arg1	nanofibers					850:859	pure CAB nanofibers	841:859	pure CAB nanofibers	841:859	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	4	35	theme	composite	546:554	arg1	nanofibers					556:565	the composite nanofibers	542:565	the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG)	542:619	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	11	36	theme	better	1319:1324	arg1	attachment					1331:1340	better cell attachment	1319:1340	better cell attachment compared to pure CAB nanofibers	1319:1372	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	2	37	theme	cellulose	244:252	arg1	esters					254:259	the common cellulose esters	233:259	the common cellulose esters	233:259	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	0	38	theme	glycol	52:57	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	2	39	theme	common	237:242	arg1	esters					254:259	the common cellulose esters	233:259	the common cellulose esters	233:259	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	4	40	theme	CAB	498:500	arg1	nanofibers					502:511	CAB nanofibers	498:511	CAB nanofibers	498:511	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	3	41	theme	cell	450:453	arg1	adhesion					455:462	cell adhesion	450:462	cell adhesion	450:462	However, the intrinsic hydrophobicity of CAB limits its application in tissue engineering as it retards cell adhesion.
31884080	11	42	theme	CAB/PEG	1295:1301	arg1	nanofibers					1303:1312	CAB/PEG nanofibers	1295:1312	CAB/PEG nanofibers	1295:1312	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	4	43	theme	nanofibers	502:511	arg1	properties					484:493	the properties	480:493	the properties of CAB nanofibers	480:511	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	8	44	theme	rapid	1080:1084	arg1	biodegradation					1086:1099	more rapid biodegradation	1075:1099	more rapid biodegradation	1075:1099	As the hydrophilicity increases, the swelling ability of the composite nanofiber increases by 2-fold with more rapid biodegradation.
31884080	6	45	theme	tensile	819:825	arg1	strength					827:834	2-fold higher tensile strength	805:834	2-fold higher tensile strength	805:834	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	0	46	theme	CAB/PEG	60:66	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	12	47	used	used	1446:1449	arg2	nanofibers					1414:1423	CAB/PEG composite nanofibers	1396:1423	CAB/PEG composite nanofibers	1396:1423	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	12	47	used	used	1446:1449	arg2	scaffold					1466:1473	a nanofiber scaffold	1454:1473	a nanofiber scaffold for applications in tissue engineering	1454:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	5	48	dep	smooth	703:708	arg1	nanofibers					724:733	nanofibers	724:733	nanofibers	724:733	Different ratios of CAB to PEG were tested and only the ratio of 2:1 resulted in smooth and bead-free nanofibers.
31884080	6	49	theme	higher	812:817	arg1	strength					827:834	2-fold higher tensile strength	805:834	2-fold higher tensile strength	805:834	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	5	50	theme	Different	622:630	arg1	ratios					632:637	Different ratios	622:637	Different ratios of CAB to PEG	622:651	Different ratios of CAB to PEG were tested and only the ratio of 2:1 resulted in smooth and bead-free nanofibers.
31884080	10	51	theme	assay	1216:1220	arg1	results					1222:1228	The cell viability assay results	1197:1228	The cell viability assay results	1197:1228	The cell viability assay results revealed that the nanofibers are non-toxic.
31884080	12	52	theme	CAB/PEG	1396:1402	arg1	scaffold					1466:1473	a nanofiber scaffold	1454:1473	a nanofiber scaffold for applications in tissue engineering	1454:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	12	52	theme	CAB/PEG	1396:1402	arg1	nanofibers					1414:1423	CAB/PEG composite nanofibers	1396:1423	CAB/PEG composite nanofibers	1396:1423	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	2	53	theme	acetate	272:278	arg1	CAB					290:292	CAB	290:292	CAB	290:292	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	53	theme	acetate	272:278	arg1	butyrate					280:287	cellulose acetate butyrate	262:287	cellulose acetate butyrate (CAB)	262:293	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	53	theme	acetate	272:278	arg1	esters					254:259	the common cellulose esters	233:259	the common cellulose esters	233:259	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	53	theme	acetate	272:278	arg1	One					226:228	One	226:228	One	226:228	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	7	54	theme	water	939:943	arg1	analysis					959:966	the water contact angle analysis	935:966	the water contact angle analysis	935:966	The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis.
31884080	7	55	theme	composite	888:896	arg1	nanofibers					898:907	the composite nanofibers	884:907	the composite nanofibers	884:907	The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis.
31884080	4	56	theme	polyethylene	595:606	arg1	PEG					616:618	PEG	616:618	PEG	616:618	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	4	56	theme	polyethylene	595:606	arg1	glycol					608:613	CAB and hydrophilic polyethylene glycol	575:613	CAB and hydrophilic polyethylene glycol (PEG)	575:619	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	12	57	theme	nanofiber	1456:1464	arg1	nanofibers					1414:1423	CAB/PEG composite nanofibers	1396:1423	CAB/PEG composite nanofibers	1396:1423	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	12	57	theme	nanofiber	1456:1464	arg1	scaffold					1466:1473	a nanofiber scaffold	1454:1473	a nanofiber scaffold for applications in tissue engineering	1454:1512	Based on this study, CAB/PEG composite nanofibers could potentially be used as a nanofiber scaffold for applications in tissue engineering.
31884080	2	58	theme	cellulose	262:270	arg1	CAB					290:292	CAB	290:292	CAB	290:292	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	58	theme	cellulose	262:270	arg1	butyrate					280:287	cellulose acetate butyrate	262:287	cellulose acetate butyrate (CAB)	262:293	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	58	theme	cellulose	262:270	arg1	esters					254:259	the common cellulose esters	233:259	the common cellulose esters	233:259	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	58	theme	cellulose	262:270	arg1	One					226:228	One	226:228	One	226:228	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	9	59	theme	nanofibers	1130:1139	arg1	biocompatibility					1106:1121	The biocompatibility	1102:1121	The biocompatibility of the nanofibers	1102:1139	The biocompatibility of the nanofibers was tested with normal human dermal fibroblasts (NHDF).
31884080	6	60	contain	have	800:803	arg1	nanofibers					789:798	CAB/PEG composite nanofibers	771:798	CAB/PEG composite nanofibers	771:798	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	6	60	contain	have	800:803	arg2	strength					827:834	2-fold higher tensile strength	805:834	2-fold higher tensile strength	805:834	The tensile test results show that CAB/PEG composite nanofibers have 2-fold higher tensile strength than pure CAB nanofibers.
31884080	10	61	theme	cell	1201:1204	arg1	results					1222:1228	The cell viability assay results	1197:1228	The cell viability assay results	1197:1228	The cell viability assay results revealed that the nanofibers are non-toxic.
31884080	4	62	theme	hydrophilic	583:593	arg1	PEG					616:618	PEG	616:618	PEG	616:618	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	4	62	theme	hydrophilic	583:593	arg1	glycol					608:613	CAB and hydrophilic polyethylene glycol	575:613	CAB and hydrophilic polyethylene glycol (PEG)	575:619	In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG).
31884080	8	63	theme	composite	1030:1038	arg1	nanofiber					1040:1048	the composite nanofiber	1026:1048	the composite nanofiber	1026:1048	As the hydrophilicity increases, the swelling ability of the composite nanofiber increases by 2-fold with more rapid biodegradation.
31884080	11	64	contain	have	1314:1317	arg1	nanofibers					1303:1312	CAB/PEG nanofibers	1295:1312	CAB/PEG nanofibers	1295:1312	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	11	64	contain	have	1314:1317	arg2	attachment					1331:1340	better cell attachment	1319:1340	better cell attachment compared to pure CAB nanofibers	1319:1372	In addition to that, CAB/PEG nanofibers have better cell attachment compared to pure CAB nanofibers.
31884080	10	65	theme	viability	1206:1214	arg1	results					1222:1228	The cell viability assay results	1197:1228	The cell viability assay results	1197:1228	The cell viability assay results revealed that the nanofibers are non-toxic.
31884080	0	66	theme	composite	69:77	arg1	nanofibers					79:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers	0:88	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.	0:134	Electrospun cellulose acetate butyrate/polyethylene glycol (CAB/PEG) composite nanofibers: A potential scaffold for tissue engineering.
31884080	2	67	theme	esters	254:259	arg1	butyrate					280:287	cellulose acetate butyrate	262:287	cellulose acetate butyrate (CAB)	262:293	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	67	theme	esters	254:259	arg1	esters					254:259	the common cellulose esters	233:259	the common cellulose esters	233:259	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	2	67	theme	esters	254:259	arg1	One					226:228	One	226:228	One	226:228	One of the common cellulose esters, cellulose acetate butyrate (CAB), has been electrospun into nanofibers and studied.
31884080	7	68	theme	contact	945:951	arg1	analysis					959:966	the water contact angle analysis	935:966	the water contact angle analysis	935:966	The hydrophobicity of the composite nanofibers was also reduced based on the water contact angle analysis.
31884080	1	69	theme	common	157:162	arg1	Electrospinning					136:150	Electrospinning	136:150	Electrospinning	136:150	Electrospinning is a common method to prepare nanofiber scaffolds for tissue engineering.
31884080	1	69	theme	common	157:162	arg1	method					164:169	a common method	155:169	a common method to prepare nanofiber scaffolds for tissue engineering	155:223	Electrospinning is a common method to prepare nanofiber scaffolds for tissue engineering.
31158062	0	0	theme	magnetic	75:82	arg1	composites					84:93	carbon magnetic composites	68:93	carbon magnetic composites prepared from sugarcane bagasse by microwave method	68:145	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	7	1	contain	had	1067:1069	arg1	pH					1064:1065	the pH	1060:1065	the pH	1060:1065	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	7	1	contain	had	1067:1069	arg2	effect					1086:1091	no significant effect	1071:1091	no significant effect	1071:1091	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	4	2	theme	greater	601:607	arg1	groups					635:640	acid functional groups	619:640	acid functional groups	619:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	4	2	theme	greater	601:607	arg1	amount					609:614	a greater amount	599:614	a greater amount of acid functional groups	599:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	10	3	theme	adsorption	1370:1379	arg1	behaviour					1381:1389	the adsorption behaviour	1366:1389	the adsorption behaviour was an endothermic process	1366:1416	The positive value of ΔH revealed the adsorption behaviour was an endothermic process.
31158062	1	4	theme	magnetic	227:234	arg1	bagasse					246:252	magnetic sugarcane bagasse	227:252	magnetic sugarcane bagasse activated carbon (MSBAC)	227:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	0	5	theme	carbon	68:73	arg1	composites					84:93	carbon magnetic composites	68:93	carbon magnetic composites prepared from sugarcane bagasse by microwave method	68:145	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	1	6	theme	simple	315:320	arg1	method					332:337	a simple microwave method	313:337	a simple microwave method	313:337	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	1	7	theme	sugarcane	236:244	arg1	bagasse					246:252	magnetic sugarcane bagasse	227:252	magnetic sugarcane bagasse activated carbon (MSBAC)	227:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	6	8	theme	regeneration	930:941	arg1	time					877:880	time	877:880	time on the adsorption of MB	877:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	8	theme	regeneration	930:941	arg1	Effects					809:815	Effects	809:815	Effects of pH, MSBAC dosage, initial MB concentration, temperature	809:874	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	8	theme	regeneration	930:941	arg1	possibility					915:925	the possibility	911:925	the possibility of regeneration of MSBAC	911:950	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	1	9	theme	microwave	322:330	arg1	method					332:337	a simple microwave method	313:337	a simple microwave method	313:337	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	13	10	theme	calcination	1577:1587	arg1	method					1589:1594	a simple calcination method	1568:1594	a simple calcination method	1568:1594	The used MSBAC could be regenerated by a simple calcination method under the temperature of 300℃ for 30 min.
31158062	8	11	theme	kinetic	1202:1208	arg1	data					1210:1213	the adsorption kinetic data	1187:1213	the adsorption kinetic data	1187:1213	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	1	12	theme	bagasse	246:252	arg1	MSBAC					272:276	MSBAC	272:276	MSBAC	272:276	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	1	12	theme	bagasse	246:252	arg1	carbon					264:269	magnetic sugarcane bagasse activated carbon	227:269	magnetic sugarcane bagasse activated carbon (MSBAC)	227:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	7	13	theme	MB	1100:1101	arg1	adsorption					1103:1112	the MB adsorption	1096:1112	the MB adsorption in the range of 2-10	1096:1133	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	6	14	from	time	877:880	arg1	adsorption					889:898	the adsorption	885:898	the adsorption of MB	885:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	15	from	possibility	915:925	arg1	adsorption					889:898	the adsorption	885:898	the adsorption of MB	885:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	3	16	theme	loose	484:488	arg1	structure					490:498	a loose structure	482:498	a loose structure	482:498	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	12	17	theme	magnetic	1496:1503	arg1	performance					1516:1526	a good magnetic separation performance	1489:1526	a good magnetic separation performance	1489:1526	MSBAC had a good magnetic separation performance.
31158062	1	18	theme	activated	254:262	arg1	MSBAC					272:276	MSBAC	272:276	MSBAC	272:276	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	1	18	theme	activated	254:262	arg1	carbon					264:269	magnetic sugarcane bagasse activated carbon	227:269	magnetic sugarcane bagasse activated carbon (MSBAC)	227:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	0	19	theme	sugarcane	109:117	arg1	bagasse					119:125	sugarcane bagasse	109:125	sugarcane bagasse	109:125	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	7	20	theme	maximum	1010:1016	arg1	36.14 mg·g-1					1042:1053	36.14 mg·g-1	1042:1053	36.14 mg·g-1	1042:1053	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	7	20	theme	maximum	1010:1016	arg1	capacity					1029:1036	the maximum adsorption capacity	1006:1036	the maximum adsorption capacity	1006:1036	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	9	21	theme	film	1316:1319	arg1	diffusion					1321:1329	film diffusion	1316:1329	film diffusion	1316:1329	The adsorption process involving the surface diffusion and film diffusion.
31158062	2	22	dep	composition	344:354	arg1	The					340:342	The	340:342	The	340:342	The composition and structure of MSBAC were characterised by SEM, XRD, BET, and FT-IR.
31158062	5	23	theme	sample	791:796	arg1	methylene					755:763	methylene blue	755:768	methylene blue (MB)	755:773	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	5	23	theme	sample	791:796	arg1	pollutant					798:806	sample pollutant	791:806	sample pollutant	791:806	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	2	24	theme	MSBAC	373:377	arg1	structure					360:368	structure	360:368	structure	360:368	The composition and structure of MSBAC were characterised by SEM, XRD, BET, and FT-IR.
31158062	2	24	theme	MSBAC	373:377	arg1	composition					344:354	composition	344:354	composition	344:354	The composition and structure of MSBAC were characterised by SEM, XRD, BET, and FT-IR.
31158062	6	25	theme	initial	838:844	arg1	concentration					849:861	initial MB concentration	838:861	initial MB concentration	838:861	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	25	theme	initial	838:844	arg1	pH					820:821	pH	820:821	pH	820:821	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	0	26	from	water	32:36	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of cationic dye from water using an iron	0:50	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	11	27	theme	salt	1423:1426	arg1	concentration					1428:1440	The salt concentration	1419:1440	The salt concentration	1419:1440	The salt concentration had a negative effect on MB removal.
31158062	7	28	theme	adsorption	1018:1027	arg1	36.14 mg·g-1					1042:1053	36.14 mg·g-1	1042:1053	36.14 mg·g-1	1042:1053	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	7	28	theme	adsorption	1018:1027	arg1	capacity					1029:1036	the maximum adsorption capacity	1006:1036	the maximum adsorption capacity	1006:1036	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	13	29	theme	used	1533:1536	arg1	MSBAC					1538:1542	The used MSBAC	1529:1542	The used MSBAC	1529:1542	The used MSBAC could be regenerated by a simple calcination method under the temperature of 300℃ for 30 min.
31158062	7	30	from	adsorption	1103:1112	arg1	range					1121:1125	the range	1117:1125	the range of 2-10	1117:1133	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	3	31	theme	rough	504:508	arg1	surface					510:516	rough surface	504:516	rough surface	504:516	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	12	32	theme	separation	1505:1514	arg1	performance					1516:1526	a good magnetic separation performance	1489:1526	a good magnetic separation performance	1489:1526	MSBAC had a good magnetic separation performance.
31158062	9	33	theme	surface	1294:1300	arg1	diffusion					1302:1310	surface diffusion	1294:1310	surface diffusion	1294:1310	The adsorption process involving the surface diffusion and film diffusion.
31158062	1	34	theme	carbon	264:269	arg1	biomass					216:222	new biomass	212:222	new biomass of magnetic sugarcane bagasse activated carbon (MSBAC)	212:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	9	35	theme	adsorption	1261:1270	arg1	process					1272:1278	The adsorption process	1257:1278	The adsorption process	1257:1278	The adsorption process involving the surface diffusion and film diffusion.
31158062	4	36	theme	groups	635:640	arg1	groups					635:640	acid functional groups	619:640	acid functional groups	619:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	4	36	theme	groups	635:640	arg1	amount					609:614	a greater amount	599:614	a greater amount of acid functional groups	599:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	1	37	theme	agricultural	171:182	arg1	waste					184:188	an agricultural waste	168:188	an agricultural waste of sugarcane bagasse	168:209	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	0	38	theme	dye	23:25	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of cationic dye from water using an iron	0:50	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	8	39	theme	pseudo-second-order	1222:1240	arg1	model					1250:1254	pseudo-second-order kinetic model	1222:1254	pseudo-second-order kinetic model	1222:1254	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	6	40	theme	MB	903:904	arg1	adsorption					889:898	the adsorption	885:898	the adsorption of MB	885:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	0	41	theme	cationic	14:21	arg1	dye					23:25	cationic dye	14:25	cationic dye	14:25	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	6	42	theme	MSBAC	824:828	arg1	pH					820:821	pH	820:821	pH	820:821	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	42	theme	MSBAC	824:828	arg1	dosage					830:835	MSBAC dosage	824:835	MSBAC dosage	824:835	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	4	43	theme	basic	647:651	arg1	groups					653:658	basic groups	647:658	basic groups	647:658	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	8	44	theme	adsorption	1191:1200	arg1	data					1210:1213	the adsorption kinetic data	1187:1213	the adsorption kinetic data	1187:1213	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	5	45	theme	adsorption	726:735	arg1	ability					737:743	the adsorption ability	722:743	the adsorption ability of MSBAC	722:752	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	3	46	with	material	468:475	arg1	structure					490:498	a loose structure	482:498	a loose structure	482:498	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	12	47	theme	good	1491:1494	arg1	performance					1516:1526	a good magnetic separation performance	1489:1526	a good magnetic separation performance	1489:1526	MSBAC had a good magnetic separation performance.
31158062	10	48	theme	positive	1336:1343	arg1	value					1345:1349	The positive value	1332:1349	The positive value of ΔH	1332:1355	The positive value of ΔH revealed the adsorption behaviour was an endothermic process.
31158062	8	49	theme	equilibrium	1140:1150	arg1	data					1152:1155	The equilibrium data	1136:1155	The equilibrium data	1136:1155	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	5	50	theme	blue	765:768	arg1	methylene					755:763	methylene blue	755:768	methylene blue (MB)	755:773	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	5	50	theme	blue	765:768	arg1	MB					771:772	MB	771:772	MB	771:772	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	5	50	theme	blue	765:768	arg1	pollutant					798:806	sample pollutant	791:806	sample pollutant	791:806	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	1	51	theme	sugarcane	193:201	arg1	bagasse					203:209	sugarcane bagasse	193:209	sugarcane bagasse	193:209	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	5	52	theme	MSBAC	748:752	arg1	ability					737:743	the adsorption ability	722:743	the adsorption ability of MSBAC	722:752	To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant.
31158062	6	53	theme	pH	820:821	arg1	time					877:880	time	877:880	time on the adsorption of MB	877:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	53	theme	pH	820:821	arg1	Effects					809:815	Effects	809:815	Effects of pH, MSBAC dosage, initial MB concentration, temperature	809:874	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	53	theme	pH	820:821	arg1	possibility					915:925	the possibility	911:925	the possibility of regeneration of MSBAC	911:950	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	0	54	theme	microwave	130:138	arg1	method					140:145	microwave method	130:145	microwave method	130:145	Adsorption of cationic dye from water using an iron oxide/activated carbon magnetic composites prepared from sugarcane bagasse by microwave method.
31158062	4	55	theme	dye	708:710	arg1	adsorption					685:694	adsorption	685:694	adsorption of cationic dye	685:710	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	1	56	theme	bagasse	203:209	arg1	waste					184:188	an agricultural waste	168:188	an agricultural waste of sugarcane bagasse	168:209	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	7	57	theme	adsorption	975:984	arg1	results					986:992	The adsorption results	971:992	The adsorption results	971:992	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	11	58	contain	had	1442:1444	arg1	concentration					1428:1440	The salt concentration	1419:1440	The salt concentration	1419:1440	The salt concentration had a negative effect on MB removal.
31158062	11	58	contain	had	1442:1444	arg2	effect					1457:1462	a negative effect	1446:1462	a negative effect	1446:1462	The salt concentration had a negative effect on MB removal.
31158062	6	59	from	Effects	809:815	arg1	adsorption					889:898	the adsorption	885:898	the adsorption of MB	885:904	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	11	60	theme	negative	1448:1455	arg1	effect					1457:1462	a negative effect	1446:1462	a negative effect	1446:1462	The salt concentration had a negative effect on MB removal.
31158062	8	61	theme	kinetic	1242:1248	arg1	model					1250:1254	pseudo-second-order kinetic model	1222:1254	pseudo-second-order kinetic model	1222:1254	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	13	62	theme	300℃	1621:1624	arg1	temperature					1606:1616	the temperature	1602:1616	the temperature of 300℃ for 30 min	1602:1635	The used MSBAC could be regenerated by a simple calcination method under the temperature of 300℃ for 30 min.
31158062	7	63	theme	significant	1074:1084	arg1	effect					1086:1091	no significant effect	1071:1091	no significant effect	1071:1091	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	3	64	theme	specific	537:544	arg1	area					554:557	a high specific surface area	530:557	a high specific surface area	530:557	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	4	65	theme	functional	624:633	arg1	groups					635:640	acid functional groups	619:640	acid functional groups	619:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	6	66	theme	MB	846:847	arg1	concentration					849:861	initial MB concentration	838:861	initial MB concentration	838:861	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	6	66	theme	MB	846:847	arg1	pH					820:821	pH	820:821	pH	820:821	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	10	67	theme	ΔH	1354:1355	arg1	value					1345:1349	The positive value	1332:1349	The positive value of ΔH	1332:1355	The positive value of ΔH revealed the adsorption behaviour was an endothermic process.
31158062	3	68	theme	surface	546:552	arg1	area					554:557	a high specific surface area	530:557	a high specific surface area	530:557	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	11	69	theme	MB	1467:1468	arg1	removal					1470:1476	MB removal	1467:1476	MB removal	1467:1476	The salt concentration had a negative effect on MB removal.
31158062	4	70	theme	acid	619:622	arg1	groups					635:640	acid functional groups	619:640	acid functional groups	619:640	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	8	71	theme	Langmuir	1164:1171	arg1	isotherm					1173:1180	Langmuir isotherm	1164:1180	Langmuir isotherm	1164:1180	The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model.
31158062	1	72	theme	new	212:214	arg1	biomass					216:222	new biomass	212:222	new biomass of magnetic sugarcane bagasse activated carbon (MSBAC)	212:277	In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method.
31158062	10	73	theme	endothermic	1398:1408	arg1	process					1410:1416	an endothermic process	1395:1416	an endothermic process	1395:1416	The positive value of ΔH revealed the adsorption behaviour was an endothermic process.
31158062	13	74	theme	simple	1570:1575	arg1	method					1589:1594	a simple calcination method	1568:1594	a simple calcination method	1568:1594	The used MSBAC could be regenerated by a simple calcination method under the temperature of 300℃ for 30 min.
31158062	4	75	theme	cationic	699:706	arg1	dye					708:710	cationic dye	699:710	cationic dye	699:710	The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye.
31158062	7	76	theme	2-10	1130:1133	arg1	range					1121:1125	the range	1117:1125	the range of 2-10	1117:1133	The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10.
31158062	6	77	theme	MSBAC	946:950	arg1	regeneration					930:941	regeneration	930:941	regeneration of MSBAC	930:950	Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated.
31158062	3	78	contain	had	526:528	arg1	it					523:524	it	523:524	it	523:524	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	3	78	contain	had	526:528	arg2	area					554:557	a high specific surface area	530:557	a high specific surface area	530:557	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	12	79	contain	had	1485:1487	arg2	performance					1516:1526	a good magnetic separation performance	1489:1526	a good magnetic separation performance	1489:1526	MSBAC had a good magnetic separation performance.
31158062	12	79	contain	had	1485:1487	arg1	MSBAC					1479:1483	MSBAC	1479:1483	MSBAC	1479:1483	MSBAC had a good magnetic separation performance.
31158062	3	80	theme	high	532:535	arg1	area					554:557	a high specific surface area	530:557	a high specific surface area	530:557	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	3	81	theme	mesoporous	457:466	arg1	material					468:475	a mesoporous material	455:475	a mesoporous material with a loose structure	455:498	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	3	81	theme	mesoporous	457:466	arg1	MSBAC					445:449	MSBAC	445:449	MSBAC	445:449	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
31158062	3	82	with	surface	510:516	arg1	structure					490:498	a loose structure	482:498	a loose structure	482:498	It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area.
30300697	0	0	theme	electrospun	85:95	arg1	scaffold					107:114	conductive electrospun composite scaffold	74:114	conductive electrospun composite scaffold for neural tissue engineering	74:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	0	1	from	effect	18:23	arg1	bioactivity					59:69	bioactivity	59:69	bioactivity	59:69	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	0	1	from	effect	18:23	arg1	hydrophilicity					40:53	hydrophilicity	40:53	hydrophilicity	40:53	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	5	2	from	increase	1167:1174	arg1	proliferation					1179:1191	proliferation	1179:1191	proliferation	1179:1191	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	0	3	theme	conductive	74:83	arg1	scaffold					107:114	conductive electrospun composite scaffold	74:114	conductive electrospun composite scaffold for neural tissue engineering	74:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	3	4	theme	contact	721:727	arg1	angle					729:733	contact angle	721:733	contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan)	721:796	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	4	5	dep	36 nm	1009:1013	arg1	to					1006:1007	to	1006:1007	to	1006:1007	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	3	6	theme	scaffold	682:689	arg1	hydrophilicity					660:673	the hydrophilicity	656:673	the hydrophilicity of the scaffold	656:689	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	5	7	theme	PC12	1255:1258	arg1	PCL					1226:1228	Pure PCL	1221:1228	Pure PCL	1221:1228	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	5	7	theme	PC12	1255:1258	arg1	extension					1242:1250	neurite extension	1234:1250	neurite extension	1234:1250	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	4	8	from	increase	930:937	arg1	content					961:967	chitosan content	952:967	chitosan content	952:967	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	0	9	theme	scaffold	107:114	arg1	bioactivity					59:69	bioactivity	59:69	bioactivity	59:69	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	0	9	theme	scaffold	107:114	arg1	hydrophilicity					40:53	hydrophilicity	40:53	hydrophilicity	40:53	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	1	10	theme	versatile	345:353	arg1	advantages					355:364	versatile advantages	345:364	versatile advantages of chitosan and PPy	345:384	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	7	11	dep	tissue	1452:1457	arg1	substitutes					1459:1469	substitutes	1459:1469	substitutes	1459:1469	Therefore, this scaffold could serve as promising neural tissue substitutes.
30300697	7	12	theme	promising	1435:1443	arg1	tissue					1452:1457	promising neural tissue substitutes	1435:1469	promising neural tissue substitutes	1435:1469	Therefore, this scaffold could serve as promising neural tissue substitutes.
30300697	5	13	theme	PCL/chitosan/PPy	1068:1083	arg1	scaffold					1097:1104	the PCL/chitosan/PPy nanofibrous scaffold	1064:1104	the PCL/chitosan/PPy nanofibrous scaffold	1064:1104	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	0	14	theme	composite	97:105	arg1	scaffold					107:114	conductive electrospun composite scaffold	74:114	conductive electrospun composite scaffold for neural tissue engineering	74:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	5	15	theme	nanofibrous	1085:1095	arg1	scaffold					1097:1104	the PCL/chitosan/PPy nanofibrous scaffold	1064:1104	the PCL/chitosan/PPy nanofibrous scaffold	1064:1104	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	5	16	theme	significant	1155:1165	arg1	increase					1167:1174	revealed significant increase	1146:1174	revealed significant increase in proliferation	1146:1191	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	7	17	theme	neural	1445:1450	arg1	tissue					1452:1457	promising neural tissue substitutes	1435:1469	promising neural tissue substitutes	1435:1469	Therefore, this scaffold could serve as promising neural tissue substitutes.
30300697	0	18	theme	neural	120:125	arg1	engineering					134:144	neural tissue engineering	120:144	neural tissue engineering	120:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	1	19	with	advantages	292:301	arg1	advantages					355:364	versatile advantages	345:364	versatile advantages of chitosan and PPy	345:384	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	4	20	theme	chitosan	914:921	arg1	concentration					893:905	the concentration	889:905	the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm	889:1013	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	1	21	theme	chitosan	369:376	arg1	advantages					355:364	versatile advantages	345:364	versatile advantages of chitosan and PPy	345:384	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	6	22	dep	adhesion	1338:1345	arg1	the					1334:1336	the	1334:1336	the	1334:1336	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	2	23	theme	Various	387:393	arg1	compositions					395:406	Various compositions	387:406	Various compositions of the PCL/chitosan/PPy polymeric scaffolds	387:450	Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity.
30300697	5	24	theme	cell	1115:1118	arg1	attachment					1120:1129	cell attachment	1115:1129	cell attachment	1115:1129	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	4	25	theme	30-180 nm	858:866	arg1	range					849:853	the range	845:853	the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm	845:1013	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	3	26	from	decrease	709:716	arg1	angle					729:733	contact angle	721:733	contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan)	721:796	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	1	27	theme	topography	329:338	arg1	advantages					292:301	advantages	292:301	advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy	292:384	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	6	28	theme	cells	1388:1392	arg1	adhesion					1338:1345	adhesion	1338:1345	adhesion	1338:1345	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	6	28	theme	cells	1388:1392	arg1	proliferation					1362:1374	proliferation	1362:1374	proliferation	1362:1374	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	6	28	theme	cells	1388:1392	arg1	spreading					1348:1356	spreading	1348:1356	spreading	1348:1356	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	4	29	dep	%	947:947	arg1	30					945:946	30	945:946	30	945:946	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	6	30	theme	PC12	1383:1386	arg1	cells					1388:1392	the PC12 cells	1379:1392	the PC12 cells	1379:1392	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	4	31	theme	fibers	827:832	arg1	diameter					811:818	The average diameter	799:818	The average diameter of the fibers	799:832	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	2	32	theme	polymeric	432:440	arg1	scaffolds					442:450	the PCL/chitosan/PPy polymeric scaffolds	411:450	the PCL/chitosan/PPy polymeric scaffolds	411:450	Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity.
30300697	2	33	theme	scaffolds	442:450	arg1	compositions					395:406	Various compositions	387:406	Various compositions of the PCL/chitosan/PPy polymeric scaffolds	387:450	Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity.
30300697	4	34	from	36 nm	1009:1013	arg1	diameter					985:992	fiber diameter	979:992	fiber diameter from 124 nm to 36 nm	979:1013	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	0	35	theme	tissue	127:132	arg1	engineering					134:144	neural tissue engineering	120:144	neural tissue engineering	120:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	1	36	theme	PPy	382:384	arg1	advantages					355:364	versatile advantages	345:364	versatile advantages of chitosan and PPy	345:384	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	5	37	dep	attachment	1120:1129	arg1	%					1202:1202	up to 356%	1193:1202	up to 356% in comparison to Pure PCL and neurite extension of PC12	1193:1258	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	2	38	theme	PCL/chitosan/PPy	415:430	arg1	scaffolds					442:450	the PCL/chitosan/PPy polymeric scaffolds	411:450	the PCL/chitosan/PPy polymeric scaffolds	411:450	Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity.
30300697	5	39	from	spreading	1132:1140	arg1	proliferation					1179:1191	proliferation	1179:1191	proliferation	1179:1191	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	5	40	from	%	1202:1202	arg1	comparison					1207:1216	comparison	1207:1216	comparison to Pure PCL and neurite extension of PC12	1207:1258	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	3	41	theme	significant	629:639	arg1	advancement					641:651	significant advancement	629:651	significant advancement in the hydrophilicity of the scaffold	629:689	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	3	42	from	advancement	641:651	arg1	hydrophilicity					660:673	the hydrophilicity	656:673	the hydrophilicity of the scaffold	656:689	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	0	43	theme	chitosan	28:35	arg1	effect					18:23	the effect	14:23	the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering	14:144	Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.
30300697	5	44	theme	revealed	1146:1153	arg1	increase					1167:1174	revealed significant increase	1146:1174	revealed significant increase in proliferation	1146:1191	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	4	45	theme	fiber	979:983	arg1	diameter					985:992	fiber diameter	979:992	fiber diameter from 124 nm to 36 nm	979:1013	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	5	46	from	attachment	1120:1129	arg1	proliferation					1179:1191	proliferation	1179:1191	proliferation	1179:1191	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	3	47	dep	%	743:743	arg1	123 ± 2.3					746:754	123 ± 2.3	746:754	123 ± 2.3	746:754	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	4	48	dep	30	945:946	arg1	to					942:943	to	942:943	to	942:943	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	1	49	contain	containing	173:182	arg2	PCL					206:208	PCL	206:208	PCL	206:208	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	1	49	contain	containing	173:182	arg2	poly					184:187	poly	184:187	poly(ε-caprolactone) (PCL)	184:209	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	1	49	contain	containing	173:182	arg1	nanofibers					162:171	nanofibers	162:171	nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy)	162:241	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	1	49	contain	containing	173:182	arg2	ε-caprolactone					189:202	ε-caprolactone	189:202	ε-caprolactone	189:202	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	1	49	contain	containing	173:182	arg2	chitosan					212:219	chitosan	212:219	chitosan	212:219	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	1	49	contain	containing	173:182	arg2	polypyrrole					225:235	polypyrrole	225:235	polypyrrole	225:235	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	5	50	dep	356	1199:1201	arg1	to					1196:1197	to	1196:1197	to	1196:1197	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	5	51	theme	In-vitro	1016:1023	arg1	studies					1025:1031	In-vitro studies	1016:1031	In-vitro studies using PC12 cells	1016:1048	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	1	52	theme	electrospun	306:316	arg1	topography					329:338	electrospun nanofibers topography	306:338	electrospun nanofibers topography	306:338	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	6	53	theme	nanofibrous	1304:1314	arg1	scaffolds					1316:1324	the PCL/chitosan/PPy nanofibrous scaffolds	1283:1324	the PCL/chitosan/PPy nanofibrous scaffolds	1283:1324	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	4	54	theme	average	803:809	arg1	diameter					811:818	The average diameter	799:818	The average diameter of the fibers	799:832	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	3	55	from	chitosan	596:603	arg1	scaffold					612:619	the scaffold	608:619	the scaffold	608:619	The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan).
30300697	5	56	theme	Pure	1221:1224	arg1	PCL					1226:1228	Pure PCL	1221:1228	Pure PCL	1221:1228	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	6	57	theme	PCL/chitosan/PPy	1287:1302	arg1	scaffolds					1316:1324	the PCL/chitosan/PPy nanofibrous scaffolds	1283:1324	the PCL/chitosan/PPy nanofibrous scaffolds	1283:1324	The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells.
30300697	5	58	theme	PC12	1039:1042	arg1	cells					1044:1048	PC12 cells	1039:1048	PC12 cells	1039:1048	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
30300697	4	59	theme	chitosan	952:959	arg1	content					961:967	chitosan content	952:967	chitosan content	952:967	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	4	60	dep	increase	930:937	arg1	%					947:947	%	947:947	%	947:947	The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm.
30300697	2	61	theme	surface	515:521	arg1	topography					523:532	their surface topography	509:532	their surface topography	509:532	Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity.
30300697	1	62	theme	nanofibers	318:327	arg1	topography					329:338	electrospun nanofibers topography	306:338	electrospun nanofibers topography	306:338	In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy.
30300697	5	63	theme	neurite	1234:1240	arg1	extension					1242:1250	neurite extension	1234:1250	neurite extension	1234:1250	In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12.
31244369	4	0	theme	inactivation	958:969	arg1	constant					976:983	the inactivation rate constant	954:983	the inactivation rate constant of the freeze-dried S. boulardii powder	954:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	4	0	theme	inactivation	958:969	arg1	k-18 = 8.04 × 10-6					1029:1046	k-18 = 8.04 × 10-6	1029:1046	k-18 = 8.04 × 10-6	1029:1046	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	6	1	theme	potential	1396:1404	arg1	prospect					1418:1425	the potential application prospect	1392:1425	the potential application prospect	1392:1425	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	6	2	theme	good	1297:1300	arg1	effect					1313:1318	a good protective effect	1295:1318	a good protective effect for the probiotic S. boulardii	1295:1349	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	5	3	theme	goat	1079:1082	arg1	results					1096:1102	the freeze-dried goat milk powder results	1062:1102	the freeze-dried goat milk powder results	1062:1102	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	4	4	theme	accelerated	911:921	arg1	test					931:934	The accelerated storage test	907:934	The accelerated storage test	907:934	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	3	5	theme	%	688:688	arg1	lactose					620:626	lactose	620:626	lactose of 21.24%	620:636	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	5	theme	%	688:688	arg1	glutamate					671:679	sodium glutamate	664:679	sodium glutamate of 4.00%	664:688	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	5	theme	%	688:688	arg1	cryoprotectants					567:581	the composite cryoprotectants	553:581	the composite cryoprotectants	553:581	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	5	theme	%	688:688	arg1	trehalose					639:647	trehalose	639:647	trehalose of 22.00%	639:657	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	1	6	dep	boulardii	159:167	arg1	boulardii					173:181	S. boulardii	170:181	S. boulardii	170:181	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	3	7	theme	expected	849:856	arg1	9.5 ± 0.07 × 109 CFU/g					797:818	9.5 ± 0.07 × 109 CFU/g	797:818	9.5 ± 0.07 × 109 CFU/g	797:818	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	7	theme	expected	849:856	arg1	rate					858:861	the expected rate	845:861	the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	845:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	1	8	theme	survival	273:280	arg1	rate					282:285	the survival rate	269:285	the survival rate of S. boulardii	269:301	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	3	9	theme	supreme	711:717	arg1	rate					728:731	the supreme survival rate	707:731	the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	707:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	10	with	%	764:764	arg1	number					787:792	the viable cell number	771:792	the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	771:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	11	theme	composite	557:565	arg1	lactose					620:626	lactose	620:626	lactose of 21.24%	620:636	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	11	theme	composite	557:565	arg1	cryoprotectants					567:581	the composite cryoprotectants	553:581	the composite cryoprotectants	553:581	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	12	theme	9.5 ± 0.07 × 109 CFU/g	797:818	arg1	number					787:792	the viable cell number	771:792	the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	771:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	0	13	theme	stability	134:142	arg1	evaluation					108:117	evaluation	108:117	evaluation	108:117	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	0	13	theme	stability	134:142	arg1	freeze-drying					90:102	freeze-drying	90:102	freeze-drying	90:102	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	3	14	theme	survival	719:726	arg1	rate					728:731	the supreme survival rate	707:731	the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	707:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	6	15	contain	has	1291:1293	arg1	formulation					1274:1284	a composite cryoprotectant formulation	1247:1284	a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry	1247:1467	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	6	15	contain	has	1291:1293	arg2	effect					1313:1318	a good protective effect	1295:1318	a good protective effect for the probiotic S. boulardii	1295:1349	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	0	16	theme	storage	126:132	arg1	stability					134:142	its storage stability	122:142	its storage stability	122:142	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	5	17	theme	powder	1089:1094	arg1	results					1096:1102	the freeze-dried goat milk powder results	1062:1102	the freeze-dried goat milk powder results	1062:1102	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	6	18	theme	cryoprotectant	1259:1272	arg1	formulation					1274:1284	a composite cryoprotectant formulation	1247:1284	a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry	1247:1467	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	3	19	dep	the	517:519	arg1	aid					521:523	aid	521:523	aid	521:523	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	5	20	theme	milk	1084:1087	arg1	results					1096:1102	the freeze-dried goat milk powder results	1062:1102	the freeze-dried goat milk powder results	1062:1102	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	4	21	theme	S.	1005:1006	arg1	powder					1018:1023	the freeze-dried S. boulardii powder	988:1023	the freeze-dried S. boulardii powder	988:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	6	22	theme	composite	1249:1257	arg1	formulation					1274:1284	a composite cryoprotectant formulation	1247:1284	a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry	1247:1467	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	3	23	theme	%	871:871	arg1	9.5 ± 0.07 × 109 CFU/g					797:818	9.5 ± 0.07 × 109 CFU/g	797:818	9.5 ± 0.07 × 109 CFU/g	797:818	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	23	theme	%	871:871	arg1	rate					858:861	the expected rate	845:861	the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	845:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	0	24	theme	Comprehensive	0:12	arg1	optimization					14:25	Comprehensive optimization	0:25	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.	0:143	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	4	25	theme	freeze-dried	992:1003	arg1	powder					1018:1023	the freeze-dried S. boulardii powder	988:1023	the freeze-dried S. boulardii powder	988:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	3	26	theme	9.6 × 109 CFU/g	890:904	arg1	number					880:885	a number	878:885	a number of 9.6 × 109 CFU/g	878:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	2	27	theme	survival	374:381	arg1	rate					383:386	the survival rate	370:386	the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage	370:491	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	2	28	theme	freeze-drying	442:454	arg1	procedure					456:464	freeze-drying procedure	442:464	freeze-drying procedure	442:464	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	3	29	theme	boulardii	739:747	arg1	rate					728:731	the supreme survival rate	707:731	the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	707:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	4	30	theme	storage	923:929	arg1	test					931:934	The accelerated storage test	907:934	The accelerated storage test	907:934	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	6	31	theme	freeze-drying	1358:1370	arg1	process					1372:1378	freeze-drying process	1358:1378	freeze-drying process	1358:1378	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	1	32	theme	diarrhea	209:216	arg1	treatment					218:226	the diarrhea treatment	205:226	the diarrhea treatment for humans or livestock	205:250	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	4	33	theme	boulardii	1008:1016	arg1	powder					1018:1023	the freeze-dried S. boulardii powder	988:1023	the freeze-dried S. boulardii powder	988:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	1	34	theme	boulardii	293:301	arg1	rate					282:285	the survival rate	269:285	the survival rate of S. boulardii	269:301	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	3	35	theme	%	657:657	arg1	lactose					620:626	lactose	620:626	lactose of 21.24%	620:636	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	35	theme	%	657:657	arg1	glutamate					671:679	sodium glutamate	664:679	sodium glutamate of 4.00%	664:688	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	35	theme	%	657:657	arg1	cryoprotectants					567:581	the composite cryoprotectants	553:581	the composite cryoprotectants	553:581	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	35	theme	%	657:657	arg1	trehalose					639:647	trehalose	639:647	trehalose of 22.00%	639:657	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	2	36	theme	composite	409:417	arg1	cryoprotectants					419:433	composite cryoprotectants	409:433	composite cryoprotectants during freeze-drying procedure and the subsequent storage	409:491	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	6	37	theme	S.	1338:1339	arg1	boulardii					1341:1349	the probiotic S. boulardii	1324:1349	the probiotic S. boulardii	1324:1349	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	6	38	theme	feed	1455:1458	arg1	industry					1460:1467	feed industry	1455:1467	feed industry	1455:1467	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	4	39	theme	powder	1018:1023	arg1	constant					976:983	the inactivation rate constant	954:983	the inactivation rate constant of the freeze-dried S. boulardii powder	954:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	4	39	theme	powder	1018:1023	arg1	k-18 = 8.04 × 10-6					1029:1046	k-18 = 8.04 × 10-6	1029:1046	k-18 = 8.04 × 10-6	1029:1046	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	0	40	theme	cryoprotectant	40:53	arg1	optimization					14:25	Comprehensive optimization	0:25	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.	0:143	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	3	41	theme	%	764:764	arg1	rate					728:731	the supreme survival rate	707:731	the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	707:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	4	42	theme	rate	971:974	arg1	constant					976:983	the inactivation rate constant	954:983	the inactivation rate constant of the freeze-dried S. boulardii powder	954:1023	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	4	42	theme	rate	971:974	arg1	k-18 = 8.04 × 10-6					1029:1046	k-18 = 8.04 × 10-6	1029:1046	k-18 = 8.04 × 10-6	1029:1046	The accelerated storage test demonstrated that the inactivation rate constant of the freeze-dried S. boulardii powder was k-18 = 8.04 × 10-6.
31244369	0	43	theme	composite	30:38	arg1	cryoprotectant					40:53	composite cryoprotectant	30:53	composite cryoprotectant	30:53	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	2	44	theme	boulardii	394:402	arg1	rate					383:386	the survival rate	370:386	the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage	370:491	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	2	45	theme	subsequent	474:483	arg1	storage					485:491	the subsequent storage	470:491	the subsequent storage	470:491	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	1	46	theme	critical	310:317	arg1	issue					319:323	the critical issue	306:323	the critical issue	306:323	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	5	47	theme	freeze-dried	1066:1077	arg1	results					1096:1102	the freeze-dried goat milk powder results	1062:1102	the freeze-dried goat milk powder results	1062:1102	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	0	48	theme	Saccharomyces	59:71	arg1	boulardii					73:81	Saccharomyces boulardii	59:81	Saccharomyces boulardii during freeze-drying and evaluation of its storage stability	59:142	Comprehensive optimization of composite cryoprotectant for Saccharomyces boulardii during freeze-drying and evaluation of its storage stability.
31244369	2	49	with	rate	383:386	arg1	cryoprotectants					419:433	composite cryoprotectants	409:433	composite cryoprotectants during freeze-drying procedure and the subsequent storage	409:491	In this study, the survival rate of S. boulardii with composite cryoprotectants during freeze-drying procedure and the subsequent storage were investigated.
31244369	5	50	theme	rate	1136:1139	arg1	k4 = 4.48 × 10-4					1156:1171	k4 = 4.48 × 10-4	1156:1171	k4 = 4.48 × 10-4	1156:1171	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	5	50	theme	rate	1136:1139	arg1	constants					1141:1149	the inactivation rate constants	1119:1149	the inactivation rate constants	1119:1149	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	6	51	dep	food	1430:1433	arg1	industry					1460:1467	feed industry	1455:1467	feed industry	1455:1467	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	3	52	with	%	871:871	arg1	number					880:885	a number	878:885	a number of 9.6 × 109 CFU/g	878:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	6	53	theme	probiotic	1328:1336	arg1	boulardii					1341:1349	the probiotic S. boulardii	1324:1349	the probiotic S. boulardii	1324:1349	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	3	54	theme	cell	782:785	arg1	number					787:792	the viable cell number	771:792	the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	771:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	6	55	theme	protective	1302:1311	arg1	effect					1313:1318	a good protective effect	1295:1318	a good protective effect for the probiotic S. boulardii	1295:1349	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	3	56	theme	sodium	664:669	arg1	glutamate					671:679	sodium glutamate	664:679	sodium glutamate of 4.00%	664:688	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	5	57	theme	inactivation	1123:1134	arg1	k4 = 4.48 × 10-4					1156:1171	k4 = 4.48 × 10-4	1156:1171	k4 = 4.48 × 10-4	1156:1171	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	5	57	theme	inactivation	1123:1134	arg1	constants					1141:1149	the inactivation rate constants	1119:1149	the inactivation rate constants	1119:1149	In addition, the freeze-dried goat milk powder results exhibited that the inactivation rate constants were k4 = 4.48 × 10-4 and k25 = 9.72 × 10-3 under 4 and 25 °C, respectively.
31244369	3	58	theme	response	528:535	arg1	method					545:550	response surface method	528:550	response surface method	528:550	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	6	59	theme	application	1406:1416	arg1	prospect					1418:1425	the potential application prospect	1392:1425	the potential application prospect	1392:1425	This work provides a composite cryoprotectant formulation that has a good protective effect for the probiotic S. boulardii during freeze-drying process, possessing the potential application prospect in food, medicine, and even feed industry.
31244369	1	60	theme	freeze-drying	332:344	arg1	process					346:352	freeze-drying process	332:352	freeze-drying process	332:352	Saccharomyces boulardii (S. boulardii) is widely adopted in the diarrhea treatment for humans or livestock, so guaranteeing the survival rate of S. boulardii is the critical issue during freeze-drying process.
31244369	3	61	theme	%	636:636	arg1	lactose					620:626	lactose	620:626	lactose of 21.24%	620:636	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	61	theme	%	636:636	arg1	glutamate					671:679	sodium glutamate	664:679	sodium glutamate of 4.00%	664:688	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	61	theme	%	636:636	arg1	cryoprotectants					567:581	the composite cryoprotectants	553:581	the composite cryoprotectants	553:581	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	61	theme	%	636:636	arg1	trehalose					639:647	trehalose	639:647	trehalose of 22.00%	639:657	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	62	theme	surface	537:543	arg1	method					545:550	response surface method	528:550	response surface method	528:550	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
31244369	3	63	theme	viable	775:780	arg1	number					787:792	the viable cell number	771:792	the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g	771:904	With the aid of response surface method, the composite cryoprotectants were comprehensively optimized to be lactose of 21.24%, trehalose of 22.00%, and sodium glutamate of 4.00%, contributing to the supreme survival rate of S. boulardii of 64.22 ± 1.35% with the viable cell number of 9.5 ± 0.07 × 109 CFU/g, which was very close to the expected rate of 65.55% with a number of 9.6 × 109 CFU/g.
30844234	7	0	theme	tumor	1546:1550	arg1	cells					1552:1556	pancreatic tumor cells	1535:1556	pancreatic tumor cells	1535:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	4	1	with	methacrylates	990:1002	arg1	degrees					1039:1045	tunable substitution degrees	1018:1045	tunable substitution degrees	1018:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	1	2	theme	great	260:264	arg1	attention					266:274	great attention	260:274	great attention in three-dimensional (3D) bioprinting	260:312	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	6	3	theme	tunable	1238:1244	arg1	spectrum					1246:1253	a wide and tunable spectrum	1227:1253	a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties	1227:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	6	4	theme	mechanical	1322:1331	arg1	properties					1333:1342	different mechanical properties	1312:1342	different mechanical properties	1312:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	8	5	theme	3D	1751:1752	arg1	materials					1773:1781	3D printing feedstock materials	1751:1781	3D printing feedstock materials	1751:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	6	theme	dermal	1512:1517	arg1	fibroblasts					1519:1529	human dermal fibroblasts	1506:1529	human dermal fibroblasts	1506:1529	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	4	7	theme	facile	874:879	arg1	approach					895:902	A facile aqueous-based approach	872:902	A facile aqueous-based approach	872:902	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	7	8	theme	seeding	1470:1476	arg1	matrices					1478:1485	the seeding matrices	1466:1485	the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells	1466:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	3	9	theme	biomimetic	725:734	arg1	aspect					736:741	the biomimetic aspect	721:741	the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure	721:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	10	theme	feedstock	1763:1771	arg1	materials					1773:1781	3D printing feedstock materials	1751:1781	3D printing feedstock materials	1751:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	1	11	theme	extracellular	172:184	arg1	matrix					186:191	the extracellular matrix	168:191	the extracellular matrix	168:191	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	6	12	from	degree	1390:1395	arg1	GGMMA					1400:1404	GGMMA	1400:1404	GGMMA	1400:1404	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	3	13	theme	heteropolysaccharides	768:788	arg1	affinity					756:763	intrinsic affinity	746:763	intrinsic affinity of heteropolysaccharides to cellulose	746:801	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	6	14	theme	different	1287:1295	arg1	hydrogels					1297:1305	different hydrogels	1287:1305	different hydrogels with different mechanical properties	1287:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	4	15	theme	UV	953:954	arg1	GGMMAs					1005:1010	GGMMAs	1005:1010	GGMMAs	1005:1010	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	4	15	theme	UV	953:954	arg1	methacrylates					990:1002	UV cross-linkable galactoglucomannan methacrylates	953:1002	UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees	953:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	3	16	theme	affinity	756:763	arg1	aspect					736:741	the biomimetic aspect	721:741	the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure	721:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	3	17	theme	engineered	560:569	arg1	inks					582:585	surface engineered biomimetic inks	552:585	surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing	552:700	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	18	theme	CNF/GGMMA	1788:1796	arg1	ink					1798:1800	the CNF/GGMMA ink	1784:1800	the CNF/GGMMA ink	1784:1800	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	4	19	theme	galactoglucomannan	971:988	arg1	GGMMAs					1005:1010	GGMMAs	1005:1010	GGMMAs	1005:1010	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	4	19	theme	galactoglucomannan	971:988	arg1	methacrylates					990:1002	UV cross-linkable galactoglucomannan methacrylates	953:1002	UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees	953:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	6	20	theme	substitution	1377:1388	arg1	degree					1390:1395	the substitution degree	1373:1395	the substitution degree in GGMMA	1373:1404	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	5	21	theme	wood-based	1134:1143	arg1	biopolymers					1145:1155	these wood-based biopolymers	1128:1155	these wood-based biopolymers	1128:1155	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	8	22	theme	tissue	1966:1971	arg1	engineering					1973:1983	tissue engineering	1966:1983	tissue engineering	1966:1983	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	5	23	theme	inks	1092:1095	arg1	window					1067:1072	The rapid gelation window	1048:1072	The rapid gelation window of the formulated inks	1048:1095	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	1	24	theme	superior	111:118	arg1	strength					131:138	their superior mechanical strength	105:138	their superior mechanical strength	105:138	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	7	25	theme	matrix	1661:1666	arg1	adhesion					1668:1675	matrix adhesion	1661:1675	matrix adhesion	1661:1675	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	4	26	theme	series	943:948	arg1	synthesis					928:936	the synthesis	924:936	the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees	924:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	3	27	theme	flexible	836:843	arg1	structure					861:869	the ultrastrong but flexible plant cell wall structure	816:869	the ultrastrong but flexible plant cell wall structure	816:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	4	28	theme	tunable	1018:1024	arg1	degrees					1039:1045	tunable substitution degrees	1018:1045	tunable substitution degrees	1018:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	6	29	dep	22.5	1275:1278	arg1	to					1272:1273	to	1272:1273	to	1272:1273	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	3	30	theme	ultrastrong	820:830	arg1	structure					861:869	the ultrastrong but flexible plant cell wall structure	816:869	the ultrastrong but flexible plant cell wall structure	816:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	31	theme	cell	1993:1996	arg1	research					1998:2005	cancer cell research	1986:2005	cancer cell research	1986:2005	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	1	32	theme	tissue	335:340	arg1	mimics					342:347	various tissue mimics	327:347	various tissue mimics	327:347	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	1	33	theme	structure	144:152	arg1	similarity					154:163	structure similarity	144:163	structure similarity	144:163	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	5	34	theme	3D	1196:1197	arg1	printing					1199:1206	extrusion-based 3D printing	1180:1206	extrusion-based 3D printing	1180:1206	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	7	35	dep	adhesion	1668:1675	arg1	the					1657:1659	the	1657:1659	the	1657:1659	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	3	36	theme	cell	851:854	arg1	structure					861:869	the ultrastrong but flexible plant cell wall structure	816:869	the ultrastrong but flexible plant cell wall structure	816:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	6	37	theme	compositional	1414:1426	arg1	ratio					1428:1432	the compositional ratio	1410:1432	the compositional ratio between GGMMA and CNFs	1410:1455	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	8	38	dep	in	1893:1894	arg1	vitro					1896:1900	vitro	1896:1900	vitro	1896:1900	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	2	39	theme	desired	393:399	arg1	performance					405:415	the desired ink performance	389:415	the desired ink performance in terms of shape fidelity and object resolution	389:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	8	40	theme	screening	2033:2041	arg1	context					1955:1961	the context	1951:1961	the context of tissue engineering, cancer cell research, and high-throughput drug screening	1951:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	6	41	from	ratio	1428:1432	arg1	GGMMA					1400:1404	GGMMA	1400:1404	GGMMA	1400:1404	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	2	42	theme	tunability	494:503	arg1	catalogue					481:489	a wide catalogue	474:489	a wide catalogue of tunability on the material property	474:528	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	0	43	theme	Surface	0:6	arg1	Inks					30:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing.
30844234	1	44	theme	three-dimensional	279:295	arg1	bioprinting					302:312	three-dimensional (3D) bioprinting	279:312	three-dimensional (3D) bioprinting	279:312	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	8	45	theme	cell-cell	1918:1926	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	46	theme	lines	1725:1729	arg1	adhesion					1668:1675	matrix adhesion	1661:1675	matrix adhesion	1661:1675	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	7	46	theme	lines	1725:1729	arg1	behaviors					1695:1703	proliferative behaviors	1681:1703	proliferative behaviors	1681:1703	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	2	47	theme	shape	429:433	arg1	fidelity					435:442	shape fidelity	429:442	shape fidelity	429:442	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	0	48	theme	Biomimetic	19:28	arg1	Inks					30:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing.
30844234	6	49	theme	wide	1229:1232	arg1	spectrum					1246:1253	a wide and tunable spectrum	1227:1253	a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties	1227:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	7	50	theme	studied	1712:1718	arg1	lines					1725:1729	the studied cell lines	1708:1729	the studied cell lines	1708:1729	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	8	51	from	variety	1882:1888	arg1	context					1955:1961	the context	1951:1961	the context of tissue engineering, cancer cell research, and high-throughput drug screening	1951:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	2	52	theme	resolution	455:464	arg1	terms					420:424	terms	420:424	terms of shape fidelity and object resolution	420:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	3	53	theme	hemicellulose	644:656	arg1	derivatives					658:668	cross-linkable hemicellulose derivatives	629:668	cross-linkable hemicellulose derivatives	629:668	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	5	54	theme	formulated	1081:1090	arg1	inks					1092:1095	the formulated inks	1077:1095	the formulated inks	1077:1095	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	7	55	theme	cells	1552:1556	arg1	cultures					1494:1501	the cultures	1490:1501	the cultures of human dermal fibroblasts and pancreatic tumor cells	1490:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	2	56	from	performance	405:415	arg1	terms					420:424	terms	420:424	terms of shape fidelity and object resolution	420:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	8	57	from	context	1955:1961	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	57	from	context	1955:1961	arg1	variety					1882:1888	a variety	1880:1888	a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1880:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	3	58	theme	extrusion	683:691	arg1	printing					693:700	UV-aided extrusion printing	674:700	UV-aided extrusion printing	674:700	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	1	59	theme	biomaterials	232:243	arg1	class					214:218	a class	212:218	a class of emerging biomaterials	212:243	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	7	60	theme	pancreatic	1535:1544	arg1	cells					1552:1556	pancreatic tumor cells	1535:1556	pancreatic tumor cells	1535:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	5	61	theme	gelation	1058:1065	arg1	window					1067:1072	The rapid gelation window	1048:1072	The rapid gelation window of the formulated inks	1048:1095	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	8	62	theme	in	1893:1894	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	63	theme	fibroblasts	1519:1529	arg1	cultures					1494:1501	the cultures	1490:1501	the cultures of human dermal fibroblasts and pancreatic tumor cells	1490:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	8	64	theme	cell-matrix	1902:1912	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	65	theme	new	1737:1739	arg1	map					1819:1821	the map	1815:1821	the map	1815:1821	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	65	theme	new	1737:1739	arg1	family					1741:1746	a new family	1735:1746	a new family of 3D printing feedstock materials	1735:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	66	theme	human	1506:1510	arg1	fibroblasts					1519:1529	human dermal fibroblasts	1506:1529	human dermal fibroblasts	1506:1529	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	3	67	theme	intrinsic	746:754	arg1	affinity					756:763	intrinsic affinity	746:763	intrinsic affinity of heteropolysaccharides to cellulose	746:801	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	6	68	theme	different	1312:1320	arg1	properties					1333:1342	different mechanical properties	1312:1342	different mechanical properties	1312:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30844234	4	69	theme	substitution	1026:1037	arg1	degrees					1039:1045	tunable substitution degrees	1018:1045	tunable substitution degrees	1018:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	8	70	theme	studies	1940:1946	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	70	theme	studies	1940:1946	arg1	variety					1882:1888	a variety	1880:1888	a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1880:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	3	71	theme	biomimetic	571:580	arg1	inks					582:585	surface engineered biomimetic inks	552:585	surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing	552:700	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	72	theme	printing	1754:1761	arg1	materials					1773:1781	3D printing feedstock materials	1751:1781	3D printing feedstock materials	1751:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	73	theme	interaction	1928:1938	arg1	studies					1940:1946	in vitro cell-matrix and cell-cell interaction studies	1893:1946	in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening	1893:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	4	74	theme	aqueous-based	881:893	arg1	approach					895:902	A facile aqueous-based approach	872:902	A facile aqueous-based approach	872:902	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	1	75	from	attention	266:274	arg1	bioprinting					302:312	three-dimensional (3D) bioprinting	279:312	three-dimensional (3D) bioprinting	279:312	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	3	76	theme	surface	552:558	arg1	inks					582:585	surface engineered biomimetic inks	552:585	surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing	552:700	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	77	theme	materials	1773:1781	arg1	map					1819:1821	the map	1815:1821	the map	1815:1821	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	77	theme	materials	1773:1781	arg1	family					1741:1746	a new family	1735:1746	a new family of 3D printing feedstock materials	1735:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	78	theme	proliferative	1681:1693	arg1	behaviors					1695:1703	proliferative behaviors	1681:1703	proliferative behaviors	1681:1703	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	2	79	from	catalogue	481:489	arg1	property					521:528	the material property	508:528	the material property	508:528	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	3	80	theme	cellulose	596:604	arg1	CNFs					619:622	CNFs	619:622	CNFs	619:622	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	3	80	theme	cellulose	596:604	arg1	nanofibrils					606:616	cellulose nanofibrils	596:616	cellulose nanofibrils (CNFs)	596:623	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	4	81	theme	cross-linkable	956:969	arg1	GGMMAs					1005:1010	GGMMAs	1005:1010	GGMMAs	1005:1010	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	4	81	theme	cross-linkable	956:969	arg1	methacrylates					990:1002	UV cross-linkable galactoglucomannan methacrylates	953:1002	UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees	953:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	8	82	theme	engineering	1973:1983	arg1	context					1955:1961	the context	1951:1961	the context of tissue engineering, cancer cell research, and high-throughput drug screening	1951:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	5	83	theme	biopolymers	1145:1155	arg1	utilization					1113:1123	the utilization	1109:1123	the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing	1109:1206	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	8	84	theme	cancer	1986:1991	arg1	research					1998:2005	cancer cell research	1986:2005	cancer cell research	1986:2005	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	1	85	theme	mechanical	120:129	arg1	strength					131:138	their superior mechanical strength	105:138	their superior mechanical strength	105:138	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	3	86	theme	plant	845:849	arg1	structure					861:869	the ultrastrong but flexible plant cell wall structure	816:869	the ultrastrong but flexible plant cell wall structure	816:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	87	theme	research	1998:2005	arg1	context					1955:1961	the context	1951:1961	the context of tissue engineering, cancer cell research, and high-throughput drug screening	1951:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	4	88	theme	methacrylates	990:1002	arg1	series					943:948	a series	941:948	a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees	941:1045	A facile aqueous-based approach was established for the synthesis of a series of UV cross-linkable galactoglucomannan methacrylates (GGMMAs) with tunable substitution degrees.
30844234	0	89	theme	3D	83:84	arg1	Printing					86:93	3D Printing	83:93	3D Printing	83:93	Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing.
30844234	5	90	theme	extrusion-based	1180:1194	arg1	printing					1199:1206	extrusion-based 3D printing	1180:1206	extrusion-based 3D printing	1180:1206	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	8	91	theme	bioinks	1826:1832	arg1	map					1819:1821	the map	1815:1821	the map	1815:1821	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	8	91	theme	bioinks	1826:1832	arg1	family					1741:1746	a new family	1735:1746	a new family of 3D printing feedstock materials	1735:1781	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	7	92	from	matrices	1478:1485	arg1	cultures					1494:1501	the cultures	1490:1501	the cultures of human dermal fibroblasts and pancreatic tumor cells	1490:1556	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	7	93	theme	great	1612:1616	arg1	cytocompatibility					1618:1634	great cytocompatibility	1612:1634	great cytocompatibility	1612:1634	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	2	94	theme	wide	476:479	arg1	catalogue					481:489	a wide catalogue	474:489	a wide catalogue of tunability on the material property	474:528	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	1	95	dep	three-dimensional	279:295	arg1	3D					298:299	3D	298:299	3D	298:299	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	8	96	theme	drug	2028:2031	arg1	screening					2033:2041	high-throughput drug screening	2012:2041	high-throughput drug screening	2012:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	2	97	theme	material	512:519	arg1	property					521:528	the material property	508:528	the material property	508:528	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	3	98	theme	wall	856:859	arg1	structure					861:869	the ultrastrong but flexible plant cell wall structure	816:869	the ultrastrong but flexible plant cell wall structure	816:869	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	8	99	theme	high-throughput	2012:2026	arg1	screening					2033:2041	high-throughput drug screening	2012:2041	high-throughput drug screening	2012:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	0	100	theme	Engineered	8:17	arg1	Inks					30:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks	0:33	Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing.
30844234	2	101	theme	fidelity	435:442	arg1	terms					420:424	terms	420:424	terms of shape fidelity and object resolution	420:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	7	102	theme	CNF/GGMMA	1590:1598	arg1	inks					1600:1603	the CNF/GGMMA inks	1586:1603	the CNF/GGMMA inks	1586:1603	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	7	103	theme	cell	1720:1723	arg1	lines					1725:1729	the studied cell lines	1708:1729	the studied cell lines	1708:1729	Used as the seeding matrices in the cultures of human dermal fibroblasts and pancreatic tumor cells, the scaffolds printed with the CNF/GGMMA inks showed great cytocompatibility as well as supported the matrix adhesion and proliferative behaviors of the studied cell lines.
30844234	8	104	from	studies	1940:1946	arg1	context					1955:1961	the context	1951:1961	the context of tissue engineering, cancer cell research, and high-throughput drug screening	1951:2041	As a new family of 3D printing feedstock materials, the CNF/GGMMA ink will broaden the map of bioinks, which potentially meets the requirements for a variety of in vitro cell-matrix and cell-cell interaction studies in the context of tissue engineering, cancer cell research, and high-throughput drug screening.
30844234	3	105	theme	cross-linkable	629:642	arg1	derivatives					658:668	cross-linkable hemicellulose derivatives	629:668	cross-linkable hemicellulose derivatives	629:668	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	2	106	theme	ink	401:403	arg1	performance					405:415	the desired ink performance	389:415	the desired ink performance in terms of shape fidelity and object resolution	389:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	5	107	theme	feeding	1164:1170	arg1	ink					1172:1174	the feeding ink	1160:1174	the feeding ink for extrusion-based 3D printing	1160:1206	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	2	108	theme	object	448:453	arg1	resolution					455:464	object resolution	448:464	object resolution	448:464	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	1	109	theme	various	327:333	arg1	mimics					342:347	various tissue mimics	327:347	various tissue mimics	327:347	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	3	110	theme	UV-aided	674:681	arg1	printing					693:700	UV-aided extrusion printing	674:700	UV-aided extrusion printing	674:700	This paper describes surface engineered biomimetic inks based on cellulose nanofibrils (CNFs) and cross-linkable hemicellulose derivatives for UV-aided extrusion printing, being inspired by the biomimetic aspect of intrinsic affinity of heteropolysaccharides to cellulose in providing the ultrastrong but flexible plant cell wall structure.
30844234	1	111	theme	emerging	223:230	arg1	biomaterials					232:243	emerging biomaterials	223:243	emerging biomaterials	223:243	Owing to their superior mechanical strength and structure similarity to the extracellular matrix, nanocelluloses as a class of emerging biomaterials have attracted great attention in three-dimensional (3D) bioprinting to fabricate various tissue mimics.
30844234	5	112	theme	rapid	1052:1056	arg1	window					1067:1072	The rapid gelation window	1048:1072	The rapid gelation window of the formulated inks	1048:1095	The rapid gelation window of the formulated inks facilitates the utilization of these wood-based biopolymers as the feeding ink for extrusion-based 3D printing.
30844234	2	113	theme	printing	360:367	arg1	complex					369:375	printing complex	360:375	printing complex	360:375	Yet, when printing complex geometries, the desired ink performance in terms of shape fidelity and object resolution demands a wide catalogue of tunability on the material property.
30844234	6	114	with	hydrogels	1297:1305	arg1	properties					1333:1342	different mechanical properties	1312:1342	different mechanical properties	1312:1342	Most importantly, a wide and tunable spectrum ranging from 2.5 to 22.5 kPa of different hydrogels with different mechanical properties could be achieved by varying the substitution degree in GGMMA and the compositional ratio between GGMMA and CNFs.
30350987	3	0	theme	unique	345:350	arg1	poly-					352:356	unique poly-	345:356	unique poly-	345:356	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	3	1	theme	different	384:392	arg1	substitutions					394:406	different substitutions	384:406	different substitutions	384:406	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	1	2	theme	marine	53:58	arg1	environment					60:70	The marine environment	49:70	The marine environment	49:70	The marine environment can increase the global production of biomass.
30350987	6	3	theme	depth	655:659	arg1	investigations					661:674	depth investigations	655:674	depth investigations of these compounds	655:693	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	4	4	theme	marine-derived	507:520	arg1	saccharides					522:532	these marine-derived saccharides	501:532	these marine-derived saccharides	501:532	There is great potential to find prebiotic compounds from these marine-derived saccharides.
30350987	3	5	dep	sulfation	415:423	arg1	e.g.					409:412	e.g.	409:412	e.g.	409:412	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	6	6	theme	novel	716:720	arg1	insights					722:729	novel insights	716:729	novel insights on the specific structures required for the observed functions	716:792	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	2	7	theme	healthy	286:292	arg1	food					294:297	healthy food	286:297	sustainability as well as healthy food	260:297	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	4	8	from	saccharides	522:532	arg1	compounds					486:494	prebiotic compounds	476:494	prebiotic compounds from these marine-derived saccharides	476:532	There is great potential to find prebiotic compounds from these marine-derived saccharides.
30350987	2	9	theme	international	210:222	arg1	agendas					224:230	international agendas	210:230	international agendas	210:230	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	2	10	theme	marine	131:136	arg1	macroalgae					138:147	marine macroalgae	131:147	marine macroalgae	131:147	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	5	11	theme	exact	548:552	arg1	composition					554:564	exact composition	548:564	exact composition	548:564	However, the exact composition and substituent distribution needed for the activity is to a large extent unexplored.
30350987	0	12	theme	Marine	0:5	arg1	Poly-					7:11	Marine Poly-	0:11	Marine Poly-	0:11	Marine Poly- and Oligosaccharides as Prebiotics.
30350987	1	13	theme	global	89:94	arg1	production					96:105	the global production	85:105	the global production of biomass	85:116	The marine environment can increase the global production of biomass.
30350987	3	14	with	poly-	352:356	arg1	substitutions					394:406	different substitutions	384:406	different substitutions	384:406	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	5	15	theme	large	627:631	arg1	extent					633:638	a large extent	625:638	a large extent	625:638	However, the exact composition and substituent distribution needed for the activity is to a large extent unexplored.
30350987	3	16	with	oligosaccharides	362:377	arg1	substitutions					394:406	different substitutions	384:406	different substitutions	384:406	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	2	17	from	Interest	119:126	arg1	macroalgae					138:147	marine macroalgae	131:147	marine macroalgae	131:147	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	2	17	from	Interest	119:126	arg1	microorganisms					153:166	microorganisms	153:166	microorganisms	153:166	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	4	18	theme	prebiotic	476:484	arg1	compounds					486:494	prebiotic compounds	476:494	prebiotic compounds from these marine-derived saccharides	476:532	There is great potential to find prebiotic compounds from these marine-derived saccharides.
30350987	6	19	from	insights	722:729	arg1	structures					747:756	the specific structures	734:756	the specific structures required for the observed functions	734:792	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	5	20	dep	composition	554:564	arg1	the					544:546	the	544:546	the	544:546	However, the exact composition and substituent distribution needed for the activity is to a large extent unexplored.
30350987	2	21	theme	trends	243:248	arg1	result					200:205	a result	198:205	a result of international agendas and market trends promoting sustainability as well as healthy food	198:297	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	2	22	theme	market	236:241	arg1	trends					243:248	market trends	236:248	market trends	236:248	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	6	23	theme	observed	775:782	arg1	functions					784:792	the observed functions	771:792	the observed functions	771:792	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	3	24	theme	marine	315:320	arg1	microorganisms					322:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	1	25	theme	biomass	110:116	arg1	production					96:105	the global production	85:105	the global production of biomass	85:116	The marine environment can increase the global production of biomass.
30350987	4	26	link	marine-derived	507:520	arg1	saccharides					522:532	these marine-derived saccharides	501:532	these marine-derived saccharides	501:532	There is great potential to find prebiotic compounds from these marine-derived saccharides.
30350987	4	27	theme	great	452:456	arg1	potential					458:466	great potential	452:466	great potential to find prebiotic compounds from these marine-derived saccharides	452:532	There is great potential to find prebiotic compounds from these marine-derived saccharides.
30350987	2	28	theme	agendas	224:230	arg1	result					200:205	a result	198:205	a result of international agendas and market trends promoting sustainability as well as healthy food	198:297	Interest in marine macroalgae and microorganisms has increased tremendously as a result of international agendas and market trends promoting sustainability as well as healthy food.
30350987	3	29	theme	Macroalgae	300:309	arg1	microorganisms					322:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	6	30	theme	specific	738:745	arg1	structures					747:756	the specific structures	734:756	the specific structures required for the observed functions	734:792	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	0	31	dep	Poly-	7:11	arg1	Prebiotics					37:46	Prebiotics	37:46	Prebiotics	37:46	Marine Poly- and Oligosaccharides as Prebiotics.
30350987	3	32	contain	contain	337:343	arg2	poly-					352:356	unique poly-	345:356	unique poly-	345:356	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	3	32	contain	contain	337:343	arg2	oligosaccharides					362:377	oligosaccharides	362:377	oligosaccharides	362:377	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	3	32	contain	contain	337:343	arg1	microorganisms					322:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms	300:335	Macroalgae and marine microorganisms contain unique poly- and oligosaccharides with different substitutions, e.g., sulfation or carboxylation.
30350987	6	33	theme	compounds	685:693	arg1	investigations					661:674	depth investigations	655:674	depth investigations of these compounds	655:693	In depth investigations of these compounds will provide us with novel insights on the specific structures required for the observed functions.
30350987	5	34	theme	substituent	570:580	arg1	distribution					582:593	substituent distribution	570:593	substituent distribution	570:593	However, the exact composition and substituent distribution needed for the activity is to a large extent unexplored.
31260763	5	0	from	addition	898:905	arg1	FDOF					934:937	the FDOF	930:937	the FDOF	930:937	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	1	1	theme	oral	174:177	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	1	theme	oral	174:177	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	0	2	theme	electrospinning	77:91	arg1	nanofibers					93:102	chitosan/pullulan electrospinning nanofibers	59:102	chitosan/pullulan electrospinning nanofibers	59:102	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	5	3	theme	melting	807:813	arg1	temperature					834:844	melting point, degradation temperature	807:844	temperature	834:844	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	5	4	theme	Thermal	778:784	arg1	analysis					786:793	Thermal analysis	778:793	Thermal analysis	778:793	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	4	5	theme	materials	767:775	arg1	crystallinity					750:762	the crystallinity	746:762	the crystallinity of materials	746:775	X-ray diffraction analysis proved that electrospinning process decreased the crystallinity of materials.
31260763	5	6	theme	point	815:819	arg1	temperature					834:844	melting point, degradation temperature	807:844	temperature	834:844	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	6	7	theme	aspirin	1102:1108	arg1	drug					1094:1097	a model drug	1086:1097	a model drug of aspirin	1086:1108	Water solubility test proved that the FDOF can dissolve in water completely within 60 s. Finally, in order to prove its practicability in future, a model drug of aspirin was encapsulated in the FDOF successfully.
31260763	4	8	theme	electrospinning	712:726	arg1	process					728:734	electrospinning process	712:734	electrospinning process	712:734	X-ray diffraction analysis proved that electrospinning process decreased the crystallinity of materials.
31260763	5	9	theme	transition	856:865	arg1	temperature					867:877	glass transition temperature	850:877	glass transition temperature	850:877	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	2	10	theme	scanning	449:456	arg1	microscopy					467:476	scanning electron microscopy	449:476	scanning electron microscopy	449:476	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	0	11	theme	dissolving	5:14	arg1	films					21:25	Fast dissolving oral films	0:25	Fast dissolving oral films for drug delivery	0:43	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	5	12	theme	glass	850:854	arg1	transition					856:865	glass transition	850:865	glass transition temperature	850:877	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	2	13	theme	nanofibers	509:518	arg1	diameter					497:504	the diameter	493:504	the diameter of nanofibers	493:518	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	14	theme	nanofiber	318:326	arg1	morphology					328:337	nanofiber morphology	318:337	nanofiber morphology	318:337	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	0	15	theme	Fast	0:3	arg1	films					21:25	Fast dissolving oral films	0:25	Fast dissolving oral films for drug delivery	0:43	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	2	16	contain	had	276:278	arg1	ratio					243:247	The ratio	239:247	The ratio of chitosan/pullulan (C/P)	239:274	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	16	contain	had	276:278	arg2	influence					283:291	an influence	280:291	an influence on solution property and nanofiber morphology	280:337	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	3	17	theme	hydrogen	605:612	arg1	interactions					619:630	hydrogen bond interactions	605:630	hydrogen bond interactions between chitosan and pullulan molecules	605:670	The Fourier transform infrared spectra indicated hydrogen bond interactions between chitosan and pullulan molecules.
31260763	3	18	dep	chitosan	640:647	arg1	molecules					662:670	molecules	662:670	molecules	662:670	The Fourier transform infrared spectra indicated hydrogen bond interactions between chitosan and pullulan molecules.
31260763	3	19	theme	infrared	578:585	arg1	spectra					587:593	infrared spectra	578:593	infrared spectra	578:593	The Fourier transform infrared spectra indicated hydrogen bond interactions between chitosan and pullulan molecules.
31260763	4	20	theme	diffraction	679:689	arg1	analysis					691:698	X-ray diffraction analysis	673:698	X-ray diffraction analysis	673:698	X-ray diffraction analysis proved that electrospinning process decreased the crystallinity of materials.
31260763	0	21	theme	oral	16:19	arg1	films					21:25	Fast dissolving oral films	0:25	Fast dissolving oral films for drug delivery	0:43	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	2	22	dep	decreased	520:528	arg1	increased					545:553	increased	545:553	decreased initially then increased	520:553	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	23	theme	solution	296:303	arg1	property					305:312	solution property	296:312	solution property	296:312	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	4	24	theme	X-ray	673:677	arg1	analysis					691:698	X-ray diffraction analysis	673:698	X-ray diffraction analysis	673:698	X-ray diffraction analysis proved that electrospinning process decreased the crystallinity of materials.
31260763	0	25	theme	drug	31:34	arg1	delivery					36:43	drug delivery	31:43	drug delivery	31:43	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	6	26	theme	solubility	946:955	arg1	test					957:960	Water solubility test	940:960	Water solubility test	940:960	Water solubility test proved that the FDOF can dissolve in water completely within 60 s. Finally, in order to prove its practicability in future, a model drug of aspirin was encapsulated in the FDOF successfully.
31260763	1	27	theme	electrospinning	211:225	arg1	technology					227:236	electrospinning technology	211:236	electrospinning technology	211:236	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	5	28	dep	temperature	834:844	arg1	degradation					822:832	melting point, degradation temperature	807:844	degradation	822:832	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	6	29	theme	Water	940:944	arg1	test					957:960	Water solubility test	940:960	Water solubility test	940:960	Water solubility test proved that the FDOF can dissolve in water completely within 60 s. Finally, in order to prove its practicability in future, a model drug of aspirin was encapsulated in the FDOF successfully.
31260763	2	30	theme	solutions	401:409	arg1	conductivity					385:396	conductivity	385:396	conductivity	385:396	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	30	theme	solutions	401:409	arg1	viscosity					371:379	viscosity	371:379	viscosity	371:379	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	3	31	theme	bond	614:617	arg1	interactions					619:630	hydrogen bond interactions	605:630	hydrogen bond interactions between chitosan and pullulan molecules	605:670	The Fourier transform infrared spectra indicated hydrogen bond interactions between chitosan and pullulan molecules.
31260763	5	32	from	FDOF	934:937	arg1	addition					898:905	the addition	894:905	the addition of chitosan content in the FDOF	894:937	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	2	33	theme	electron	458:465	arg1	microscopy					467:476	scanning electron microscopy	449:476	scanning electron microscopy	449:476	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	1	34	theme	dissolving	163:172	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	34	theme	dissolving	163:172	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	5	35	from	content	919:925	arg1	FDOF					934:937	the FDOF	930:937	the FDOF	930:937	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	1	36	theme	Chitosan/pullulan	120:136	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	36	theme	Chitosan/pullulan	120:136	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	37	theme	composite	138:146	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	37	theme	composite	138:146	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	6	38	theme	model	1088:1092	arg1	drug					1094:1097	a model drug	1086:1097	a model drug of aspirin	1086:1108	Water solubility test proved that the FDOF can dissolve in water completely within 60 s. Finally, in order to prove its practicability in future, a model drug of aspirin was encapsulated in the FDOF successfully.
31260763	5	39	theme	chitosan	910:917	arg1	content					919:925	chitosan content	910:925	chitosan content in the FDOF	910:937	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	1	40	theme	nanofiber	148:156	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	40	theme	nanofiber	148:156	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	3	41	dep	Fourier	560:566	arg1	transform					568:576	transform	568:576	transform infrared spectra	568:593	The Fourier transform infrared spectra indicated hydrogen bond interactions between chitosan and pullulan molecules.
31260763	0	42	theme	chitosan/pullulan	59:75	arg1	nanofibers					93:102	chitosan/pullulan electrospinning nanofibers	59:102	chitosan/pullulan electrospinning nanofibers	59:102	Fast dissolving oral films for drug delivery prepared from chitosan/pullulan electrospinning nanofibers.
31260763	5	43	theme	content	919:925	arg1	addition					898:905	the addition	894:905	the addition of chitosan content in the FDOF	894:937	Thermal analysis showed that melting point, degradation temperature and glass transition temperature increased with the addition of chitosan content in the FDOF.
31260763	1	44	theme	fast	158:161	arg1	FDOFs					186:190	FDOFs	186:190	FDOFs	186:190	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	1	44	theme	fast	158:161	arg1	films					179:183	Chitosan/pullulan composite nanofiber fast dissolving oral films	120:183	Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs)	120:191	In this study, Chitosan/pullulan composite nanofiber fast dissolving oral films (FDOFs) were prepared via electrospinning technology.
31260763	2	45	from	influence	283:291	arg1	property					305:312	solution property	296:312	solution property	296:312	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	45	from	influence	283:291	arg1	morphology					328:337	nanofiber morphology	318:337	nanofiber morphology	318:337	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	46	theme	chitosan/pullulan	252:268	arg1	ratio					243:247	The ratio	239:247	The ratio of chitosan/pullulan (C/P)	239:274	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31260763	2	47	theme	chitosan	361:368	arg1	increase					349:356	the increase	345:356	the increase of chitosan	345:368	The ratio of chitosan/pullulan (C/P) had an influence on solution property and nanofiber morphology, with the increase of chitosan, viscosity and conductivity of solutions increased, the morphology obtained by scanning electron microscopy indicated that the diameter of nanofibers decreased initially then increased.
31774341	0	0	theme	oligozoospermic	210:224	arg1	men					226:228	normozoospermic and oligozoospermic men	190:228	men	226:228	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	4	1	dep	populations	1317:1327	arg1	differing					1371:1379	differing	1371:1379	differing in GGT activity and surface glycans	1371:1415	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	1	dep	populations	1317:1327	arg1	exhibiting					1329:1338	exhibiting	1329:1338	exhibiting prostasome signature bands	1329:1365	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	3	2	theme	different	791:799	arg1	methods					801:807	different methods	791:807	different methods	791:807	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	5	3	theme	glycoproteins	1508:1520	arg1	profiles					1490:1497	distinct profiles	1481:1497	distinct profiles of total glycoproteins and which can be tracked down by ALP activity	1481:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	0	4	theme	glycans	141:147	arg1	context					79:85	the context	75:85	the context of concanavalin A- and wheat germ agglutinin-reactive glycans	75:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	1	5	theme	Human	243:247	arg1	Background					231:240	Background	231:240	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.	231:414	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	5	theme	Human	243:247	arg1	vesicles					315:322	intrinsically heterogeneous extracellular vesicles	273:322	intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions	273:413	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	5	theme	Human	243:247	arg1	prostasomes					257:267	Human seminal prostasomes	243:267	Human seminal prostasomes	243:267	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	0	6	theme	seminal	154:160	arg1	populations					173:183	seminal prostasome populations	154:183	seminal prostasome populations from normozoospermic and oligozoospermic men	154:228	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	4	7	theme	WGA	1439:1441	arg1	column					1443:1448	the WGA column	1435:1448	the WGA column	1435:1448	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	6	8	theme	A-separated	1676:1686	arg1	ones					1688:1691	Con A-separated ones	1672:1691	Con A-separated ones	1672:1691	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	9	theme	normozoospermic	895:909	arg1	sPro-N					912:917	normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	895:951	sPro-N	912:917	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	10	theme	normozoospermic	190:204	arg1	men					226:228	normozoospermic and oligozoospermic men	190:228	men	226:228	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	3	11	theme	prostasomes	878:888	arg1	Preparations					854:865	Preparations	854:865	Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	854:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	1	12	theme	different	380:388	arg1	conditions					404:413	different physiological conditions	380:413	different physiological conditions	380:413	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	3	13	theme	biological	673:682	arg1	activities					684:693	various biological activities	665:693	various biological activities	665:693	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	2	14	theme	lectin-affinity	558:572	arg1	chromatography					574:587	lectin-affinity chromatography	558:587	lectin-affinity chromatography (LAC)	558:593	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	2	14	theme	lectin-affinity	558:572	arg1	tool					604:607	a new tool	598:607	a new tool for their separation	598:628	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	2	14	theme	lectin-affinity	558:572	arg1	LAC					590:592	LAC	590:592	LAC	590:592	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	0	15	from	transferase	35:45	arg1	context					79:85	the context	75:85	the context of concanavalin A- and wheat germ agglutinin-reactive glycans	75:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	0	16	theme	concanavalin	90:101	arg1	A-					103:104	concanavalin A-	90:104	concanavalin A-	90:104	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	6	17	theme	alkaline	1783:1790	arg1	phosphatase					1792:1802	alkaline phosphatase	1783:1802	alkaline phosphatase	1783:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	17	theme	alkaline	1783:1790	arg1	different.Conclusions					1709:1729	different.Conclusions	1709:1729	different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase	1709:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	18	theme	glyco-parameters	712:727	arg1	introduction					696:707	introduction	696:707	introduction of glyco-parameters as reference	696:740	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	6	19	theme	prostasomes	1867:1877	arg1	populations					1879:1889	seminal prostasomes populations	1859:1889	seminal prostasomes populations from normozoospermic and oligozoospermic men	1859:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	5	20	theme	Con	1589:1591	arg1	column					1595:1600	the Con A column	1585:1600	the Con A column	1585:1600	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	4	21	theme	CD63-immunoreactive	1297:1315	arg1	populations					1317:1327	Two CD63-immunoreactive populations	1293:1327	Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans	1293:1415	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	3	22	theme	A	991:991	arg1	columns					1033:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	5	23	theme	ALP	1555:1557	arg1	activity					1559:1566	ALP activity	1555:1566	ALP activity	1555:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	2	24	theme	prostasomal	458:468	arg1	surface					470:476	the prostasomal surface	454:476	the prostasomal surface exemplified by glycan compositions as a possible distinction factor	454:544	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	0	25	theme	germ	116:119	arg1	glycans					141:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	6	26	theme	Membrane-associated	1732:1750	arg1	transferase					1767:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	26	theme	Membrane-associated	1732:1750	arg1	different.Conclusions					1709:1729	different.Conclusions	1709:1729	different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase	1709:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	2	27	theme	possible	518:525	arg1	factor					539:544	a possible distinction factor	516:544	a possible distinction factor	516:544	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	6	28	theme	WGA-reactive	1833:1844	arg1	glycans					1846:1852	WGA-reactive glycans	1833:1852	WGA-reactive glycans	1833:1852	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	4	29	theme	protein/glycoprotein	1251:1270	arg1	composition.Results					1272:1290	total protein/glycoprotein composition.Results	1245:1290	total protein/glycoprotein composition.Results	1245:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	0	30	theme	Membrane-associated	0:18	arg1	transferase					35:45	Membrane-associated gamma-glutamyl transferase	0:45	Membrane-associated gamma-glutamyl transferase	0:45	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	4	31	dep	markers	1152:1158	arg1	phosphatase					1204:1214	alkaline phosphatase	1195:1214	alkaline phosphatase (ALP)	1195:1220	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	CD63					1235:1238	tetraspanin CD63	1223:1238	tetraspanin CD63	1223:1238	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	transferase					1176:1186	gamma-glutamyl transferase	1161:1186	gamma-glutamyl transferase (GGT)	1161:1192	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	GGT					1189:1191	GGT	1189:1191	GGT	1189:1191	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	ALP					1217:1219	ALP	1217:1219	ALP	1217:1219	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	markers					1152:1158	selected markers	1143:1158	selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1143:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	31	dep	markers	1152:1158	arg1	composition.Results					1272:1290	total protein/glycoprotein composition.Results	1245:1290	total protein/glycoprotein composition.Results	1245:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	3	32	from	sPro-N	912:917	arg1	Preparations					854:865	Preparations	854:865	Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	854:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	32	from	sPro-N	912:917	arg1	prostasomes					878:888	seminal prostasomes	870:888	seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	870:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	2	33	theme	glycan	493:498	arg1	compositions					500:511	glycan compositions	493:511	glycan compositions	493:511	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	3	34	theme	Con	994:996	arg1	A					991:991	concanavalin A	978:991	concanavalin A (Con A)	978:999	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	34	theme	Con	994:996	arg1	A					998:998	Con A	994:998	Con A	994:998	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	35	theme	alkaline	51:58	arg1	phosphatase					60:70	alkaline phosphatase	51:70	alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans	51:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	3	36	theme	agglutinin	1016:1025	arg1	columns					1033:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	4	37	theme	LAC-separated	1067:1079	arg1	fractions					1081:1089	LAC-separated fractions	1067:1089	LAC-separated fractions	1067:1089	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	3	38	theme	wheat	1005:1009	arg1	agglutinin					1016:1025	wheat germ agglutinin	1005:1025	wheat germ agglutinin (WGA)	1005:1031	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	38	theme	wheat	1005:1009	arg1	WGA					1028:1030	WGA	1028:1030	WGA	1028:1030	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	4	39	theme	markers	1152:1158	arg1	distribution					1127:1138	the distribution	1123:1138	the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1123:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	40	theme	signature	1351:1359	arg1	bands					1361:1365	prostasome signature bands	1340:1365	prostasome signature bands	1340:1365	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	5	41	theme	total	1502:1506	arg1	glycoproteins					1508:1520	total glycoproteins	1502:1520	total glycoproteins and which can be tracked down by ALP activity	1502:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	4	42	theme	gamma-glutamyl	1161:1174	arg1	GGT					1189:1191	GGT	1189:1191	GGT	1189:1191	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	42	theme	gamma-glutamyl	1161:1174	arg1	markers					1152:1158	selected markers	1143:1158	selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1143:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	42	theme	gamma-glutamyl	1161:1174	arg1	transferase					1176:1186	gamma-glutamyl transferase	1161:1186	gamma-glutamyl transferase (GGT)	1161:1192	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	5	43	theme	Additional	1451:1460	arg1	populations					1462:1472	Additional populations	1451:1472	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity	1451:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	5	44	contain	having	1474:1479	arg1	populations					1462:1472	Additional populations	1451:1472	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity	1451:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	5	44	contain	having	1474:1479	arg2	profiles					1490:1497	distinct profiles	1481:1497	distinct profiles of total glycoproteins and which can be tracked down by ALP activity	1481:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	1	45	theme	heterogeneous	287:299	arg1	Background					231:240	Background	231:240	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.	231:414	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	45	theme	heterogeneous	287:299	arg1	EVs					325:327	EVs	325:327	EVs	325:327	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	45	theme	heterogeneous	287:299	arg1	vesicles					315:322	intrinsically heterogeneous extracellular vesicles	273:322	intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions	273:413	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	45	theme	heterogeneous	287:299	arg1	prostasomes					257:267	Human seminal prostasomes	243:267	Human seminal prostasomes	243:267	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	4	46	gly	protein/glycoprotein	1251:1270	arg1	protein/glycoprotein					1251:1270	total protein/glycoprotein composition.Results	1245:1290	total protein/glycoprotein composition.Results	1245:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	3	47	theme	EVs	775:777	arg1	standardization					756:770	standardization	756:770	standardization of EVs isolated by different methods and intended for use in biomedicine.Methods	756:851	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	4	48	theme	alkaline	1195:1202	arg1	markers					1152:1158	selected markers	1143:1158	selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1143:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	48	theme	alkaline	1195:1202	arg1	ALP					1217:1219	ALP	1217:1219	ALP	1217:1219	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	48	theme	alkaline	1195:1202	arg1	phosphatase					1204:1214	alkaline phosphatase	1195:1214	alkaline phosphatase (ALP)	1195:1220	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	49	theme	surface	1401:1407	arg1	glycans					1409:1415	surface glycans	1401:1415	surface glycans	1401:1415	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	2	50	theme	distinction	527:537	arg1	factor					539:544	a possible distinction factor	516:544	a possible distinction factor	516:544	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	3	51	from	LAC	971:973	arg1	columns					1033:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	concanavalin A (Con A) and wheat germ agglutinin (WGA) columns	978:1039	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	6	52	theme	WGA-separated	1603:1615	arg1	populations					1617:1627	WGA-separated populations	1603:1627	WGA-separated populations	1603:1627	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	53	from	different.Conclusions	1709:1729	arg1	context					1811:1817	the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men	1807:1934	the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men	1807:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	54	from	men	949:951	arg1	Preparations					854:865	Preparations	854:865	Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	854:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	54	from	men	949:951	arg1	prostasomes					878:888	seminal prostasomes	870:888	seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	870:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	55	theme	prostasome	162:171	arg1	populations					173:183	seminal prostasome populations	154:183	seminal prostasome populations from normozoospermic and oligozoospermic men	154:228	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	1	56	theme	seminal	249:255	arg1	Background					231:240	Background	231:240	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.	231:414	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	56	theme	seminal	249:255	arg1	vesicles					315:322	intrinsically heterogeneous extracellular vesicles	273:322	intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions	273:413	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	56	theme	seminal	249:255	arg1	prostasomes					257:267	Human seminal prostasomes	243:267	Human seminal prostasomes	243:267	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	5	57	gly	glycoproteins	1508:1520	arg1	glycoproteins					1508:1520	total glycoproteins	1502:1520	total glycoproteins and which can be tracked down by ALP activity	1502:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	6	58	theme	Con	1822:1824	arg1	A-					1826:1827	Con A-	1822:1827	Con A-	1822:1827	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	59	theme	seminal	870:876	arg1	prostasomes					878:888	seminal prostasomes	870:888	seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	870:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	4	60	theme	total	1245:1249	arg1	composition.Results					1272:1290	total protein/glycoprotein composition.Results	1245:1290	total protein/glycoprotein composition.Results	1245:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	6	61	theme	Con	1672:1674	arg1	ones					1688:1691	Con A-separated ones	1672:1691	Con A-separated ones	1672:1691	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	62	from	men	1932:1934	arg1	populations					1879:1889	seminal prostasomes populations	1859:1889	seminal prostasomes populations from normozoospermic and oligozoospermic men	1859:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	63	theme	various	665:671	arg1	activities					684:693	various biological activities	665:693	various biological activities	665:693	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	6	64	from	sPro-O	1656:1661	arg1	similar					1634:1640	similar	1634:1640	similar	1634:1640	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	65	theme	sPro-O	941:946	arg1	men					949:951	normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	895:951	men	949:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	6	66	theme	normozoospermic	1896:1910	arg1	men					1932:1934	normozoospermic and oligozoospermic men	1896:1934	men	1932:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	4	67	theme	tetraspanin	1223:1233	arg1	markers					1152:1158	selected markers	1143:1158	selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1143:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	67	theme	tetraspanin	1223:1233	arg1	CD63					1235:1238	tetraspanin CD63	1223:1238	tetraspanin CD63	1223:1238	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	0	68	theme	A-	103:104	arg1	glycans					141:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	3	69	from	Preparations	854:865	arg1	men					949:951	normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	895:951	men	949:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	69	from	Preparations	854:865	arg1	sPro-N					912:917	normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	895:951	sPro-N	912:917	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	70	theme	oligozoospermic	924:938	arg1	men					949:951	normozoospermic (sPro-N) and oligozoospermic (sPro-O) men	895:951	men	949:951	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	71	theme	wheat	110:114	arg1	germ					116:119	wheat germ	110:119	wheat germ	110:119	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	6	72	from	similar	1634:1640	arg1	sPro-O					1656:1661	sPro-O	1656:1661	sPro-O	1656:1661	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	72	from	similar	1634:1640	arg1	sPro-N					1645:1650	sPro-N	1645:1650	sPro-N	1645:1650	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	1	73	dep	different	380:388	arg1	physiological					390:402	physiological	390:402	physiological	390:402	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	6	74	theme	seminal	1859:1865	arg1	populations					1879:1889	seminal prostasomes populations	1859:1889	seminal prostasomes populations from normozoospermic and oligozoospermic men	1859:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	5	75	theme	A	1593:1593	arg1	column					1595:1600	the Con A column	1585:1600	the Con A column	1585:1600	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	4	76	theme	prostasome	1340:1349	arg1	bands					1361:1365	prostasome signature bands	1340:1365	prostasome signature bands	1340:1365	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	0	77	theme	agglutinin-reactive	121:139	arg1	glycans					141:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	concanavalin A- and wheat germ agglutinin-reactive glycans	90:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	6	78	dep	different.Conclusions	1709:1729	arg1	transferase					1767:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	78	dep	different.Conclusions	1709:1729	arg1	phosphatase					1792:1802	alkaline phosphatase	1783:1802	alkaline phosphatase	1783:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	78	dep	different.Conclusions	1709:1729	arg1	different.Conclusions					1709:1729	different.Conclusions	1709:1729	different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase	1709:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	79	theme	gamma-glutamyl	1752:1765	arg1	transferase					1767:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	Membrane-associated gamma-glutamyl transferase	1732:1777	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	79	theme	gamma-glutamyl	1752:1765	arg1	different.Conclusions					1709:1729	different.Conclusions	1709:1729	different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase	1709:1802	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	6	80	from	sPro-N	1645:1650	arg1	similar					1634:1640	similar	1634:1640	similar	1634:1640	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	0	81	from	phosphatase	60:70	arg1	context					79:85	the context	75:85	the context of concanavalin A- and wheat germ agglutinin-reactive glycans	75:147	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	3	82	from	use	826:828	arg1	biomedicine.Methods					833:851	biomedicine.Methods	833:851	biomedicine.Methods	833:851	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	83	theme	gamma-glutamyl	20:33	arg1	transferase					35:45	Membrane-associated gamma-glutamyl transferase	0:45	Membrane-associated gamma-glutamyl transferase	0:45	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	3	84	theme	concanavalin	978:989	arg1	A					991:991	concanavalin A	978:991	concanavalin A (Con A)	978:999	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	84	theme	concanavalin	978:989	arg1	A					998:998	Con A	994:998	Con A	994:998	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	85	dep	EVs	775:777	arg1	isolated					779:786	isolated	779:786	isolated by different methods	779:807	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	85	dep	EVs	775:777	arg1	intended					813:820	intended	813:820	intended for use in biomedicine.Methods	813:851	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	0	86	from	men	226:228	arg1	populations					173:183	seminal prostasome populations	154:183	seminal prostasome populations from normozoospermic and oligozoospermic men	154:228	Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
31774341	4	87	theme	GGT	1384:1386	arg1	activity					1388:1395	GGT activity	1384:1395	GGT activity	1384:1395	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	2	88	theme	molecular	430:438	arg1	properties					440:449	the molecular properties	426:449	the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor	426:544	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	5	89	theme	distinct	1481:1488	arg1	profiles					1490:1497	distinct profiles	1481:1497	distinct profiles of total glycoproteins and which can be tracked down by ALP activity	1481:1566	Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column.
31774341	2	90	theme	surface	470:476	arg1	properties					440:449	the molecular properties	426:449	the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor	426:544	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	2	91	theme	new	600:602	arg1	chromatography					574:587	lectin-affinity chromatography	558:587	lectin-affinity chromatography (LAC)	558:593	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	2	91	theme	new	600:602	arg1	tool					604:607	a new tool	598:607	a new tool for their separation	598:628	Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
31774341	6	92	theme	oligozoospermic	1916:1930	arg1	men					1932:1934	normozoospermic and oligozoospermic men	1896:1934	men	1932:1934	WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different.Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men.
31774341	3	93	theme	germ	1011:1014	arg1	agglutinin					1016:1025	wheat germ agglutinin	1005:1025	wheat germ agglutinin (WGA)	1005:1031	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	3	93	theme	germ	1011:1014	arg1	WGA					1028:1030	WGA	1028:1030	WGA	1028:1030	Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine.Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
31774341	4	94	theme	selected	1143:1150	arg1	markers					1152:1158	selected markers	1143:1158	selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results	1143:1290	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	94	theme	selected	1143:1150	arg1	transferase					1176:1186	gamma-glutamyl transferase	1161:1186	gamma-glutamyl transferase (GGT)	1161:1192	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	94	theme	selected	1143:1150	arg1	phosphatase					1204:1214	alkaline phosphatase	1195:1214	alkaline phosphatase (ALP)	1195:1220	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	4	94	theme	selected	1143:1150	arg1	CD63					1235:1238	tetraspanin CD63	1223:1238	tetraspanin CD63	1223:1238	Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition.Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column.
31774341	1	95	theme	extracellular	301:313	arg1	Background					231:240	Background	231:240	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.	231:414	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	95	theme	extracellular	301:313	arg1	EVs					325:327	EVs	325:327	EVs	325:327	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	95	theme	extracellular	301:313	arg1	vesicles					315:322	intrinsically heterogeneous extracellular vesicles	273:322	intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions	273:413	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31774341	1	95	theme	extracellular	301:313	arg1	prostasomes					257:267	Human seminal prostasomes	243:267	Human seminal prostasomes	243:267	Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions.
31593834	0	0	theme	composite	113:121	arg1	beads					123:127	composite beads	113:127	composite beads	113:127	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	13	1	theme	graphene	1639:1646	arg1	adsorbents					1648:1657	high-performance graphene adsorbents	1622:1657	high-performance graphene adsorbents	1622:1657	The implications for the design of high-performance graphene adsorbents for water and soil remediation are discussed.
31593834	5	2	theme	largest	647:653	arg1	pressure					655:662	The largest pressure	643:662	The largest pressure for GCCSC beads recorded before brittle failure	643:710	The largest pressure for GCCSC beads recorded before brittle failure was 26 N.
31593834	1	3	from	systems	210:216	arg1	pollutants					186:195	diverse pollutants	178:195	diverse pollutants from aqueous systems	178:216	Graphene adsorbents have been applied to remove diverse pollutants from aqueous systems.
31593834	9	4	theme	adsorption	1102:1111	arg1	fast					1161:1164	fast	1161:1164	fast	1161:1164	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	9	4	theme	adsorption	1102:1111	arg1	kinetics					1113:1120	The adsorption kinetics	1098:1120	The adsorption kinetics of Cu2+ on GCCSC beads	1098:1143	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	4	5	theme	larger	595:600	arg1	value					602:606	a much larger value	588:606	a much larger value than graphene oxide beads (0.2 N)	588:640	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	5	theme	larger	595:600	arg1	3.2 N					581:585	3.2 N	581:585	3.2 N	581:585	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	6	theme	proportional	543:554	arg1	3.2 N					581:585	3.2 N	581:585	3.2 N	581:585	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	6	theme	proportional	543:554	arg1	limit					556:560	The proportional limit	539:560	The proportional limit of GCCSC beads	539:575	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	13	7	theme	adsorbents	1648:1657	arg1	design					1612:1617	the design	1608:1617	the design of high-performance graphene adsorbents	1608:1657	The implications for the design of high-performance graphene adsorbents for water and soil remediation are discussed.
31593834	3	8	from	soil	533:536	arg1	immobilization					490:503	the immobilization	486:503	the immobilization of Cu2+ from both water and soil	486:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	13	9	theme	high-performance	1622:1637	arg1	adsorbents					1648:1657	high-performance graphene adsorbents	1622:1657	high-performance graphene adsorbents	1622:1657	The implications for the design of high-performance graphene adsorbents for water and soil remediation are discussed.
31593834	7	10	theme	Cu2+	934:937	arg1	adsorption					939:948	the Cu2+ adsorption	930:948	the Cu2+ adsorption onto GCCSC beads	930:965	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	3	11	theme	GCCSC	429:433	arg1	beads					446:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads	381:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil	381:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	9	12	theme	GCCSC	1133:1137	arg1	beads					1139:1143	GCCSC beads	1133:1143	GCCSC beads	1133:1143	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	3	13	theme	carboxylated	381:392	arg1	beads					446:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads	381:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil	381:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	9	14	from	kinetics	1113:1120	arg1	beads					1139:1143	GCCSC beads	1133:1143	GCCSC beads	1133:1143	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	5	15	theme	GCCSC	668:672	arg1	beads					674:678	GCCSC beads	668:678	GCCSC beads recorded before brittle failure	668:710	The largest pressure for GCCSC beads recorded before brittle failure was 26 N.
31593834	6	16	theme	initial	802:808	arg1	concentration					815:827	initial Cu2+ concentration	802:827	initial Cu2+ concentration	802:827	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	12	17	theme	immobilization	1516:1529	arg1	efficiency					1531:1540	a high Cu2+ immobilization efficiency	1504:1540	a high Cu2+ immobilization efficiency	1504:1540	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	11	18	theme	ionic	1347:1351	arg1	strength					1353:1360	The ionic strength	1343:1360	The ionic strength	1343:1360	The ionic strength did not influence the adsorption.
31593834	6	19	from	22.4 mg/g	770:778	arg1	systems					791:797	aqueous systems	783:797	aqueous systems	783:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	2	20	theme	graphene	303:310	arg1	sheets					312:317	the fragile graphene sheets	291:317	the fragile graphene sheets	291:317	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	6	21	from	concentration	815:827	arg1	22.4 mg/g					770:778	22.4 mg/g	770:778	22.4 mg/g in aqueous systems	770:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	6	21	from	concentration	815:827	arg1	capacity					742:749	The Cu2+ adsorption capacity	722:749	The Cu2+ adsorption capacity of GCCSC beads	722:764	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	1	22	theme	aqueous	202:208	arg1	systems					210:216	aqueous systems	202:216	aqueous systems	202:216	Graphene adsorbents have been applied to remove diverse pollutants from aqueous systems.
31593834	0	23	theme	soil	58:61	arg1	decontamination					11:25	Adsorptive decontamination	0:25	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene	0:86	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	2	24	theme	fragile	295:301	arg1	sheets					312:317	the fragile graphene sheets	291:317	the fragile graphene sheets	291:317	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	10	25	theme	higher	1274:1279	arg1	pH					1281:1282	higher pH	1274:1282	higher pH	1274:1282	A lower temperature and higher pH slightly increased the adsorption capacity of GCCSC beads.
31593834	3	26	theme	graphene	394:401	arg1	beads					446:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads	381:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil	381:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	6	27	theme	aqueous	783:789	arg1	systems					791:797	aqueous systems	783:797	aqueous systems	783:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	3	28	theme	good	457:460	arg1	strength					473:480	good mechanical strength	457:480	good mechanical strength	457:480	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	0	29	theme	Adsorptive	0:9	arg1	decontamination					11:25	Adsorptive decontamination	0:25	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene	0:86	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	9	30	theme	0.0021 g/	1231:1239	arg1	k2					1225:1226	a k2	1223:1226	a k2 of 0.0021 g/(mg·min)	1223:1247	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	3	31	from	water	523:527	arg1	immobilization					490:503	the immobilization	486:503	the immobilization of Cu2+ from both water and soil	486:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	3	32	theme	mechanical	462:471	arg1	strength					473:480	good mechanical strength	457:480	good mechanical strength	457:480	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	0	33	theme	Cu2+-contaminated	30:46	arg1	water					48:52	Cu2+-contaminated water	30:52	Cu2+-contaminated water	30:52	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	10	34	theme	GCCSC	1330:1334	arg1	beads					1336:1340	GCCSC beads	1330:1340	GCCSC beads	1330:1340	A lower temperature and higher pH slightly increased the adsorption capacity of GCCSC beads.
31593834	1	35	theme	Graphene	130:137	arg1	adsorbents					139:148	Graphene adsorbents	130:148	Graphene adsorbents	130:148	Graphene adsorbents have been applied to remove diverse pollutants from aqueous systems.
31593834	7	36	theme	GCCSC	955:959	arg1	beads					961:965	GCCSC beads	955:965	GCCSC beads	955:965	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	7	37	theme	qe	1007:1008	arg1	Ce					987:988	Ce	987:988	Ce of 0.83 mg/L and qe of 14.3 mg/g	987:1021	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	3	38	theme	oxide/chitosan/cellulose	403:426	arg1	beads					446:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads	381:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil	381:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	12	39	theme	GCCSC	1558:1562	arg1	beads					1564:1568	GCCSC beads	1558:1568	GCCSC beads applied to soil	1558:1584	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	3	40	with	beads	446:450	arg1	strength					473:480	good mechanical strength	457:480	good mechanical strength	457:480	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	6	41	theme	many	868:871	arg1	adsorbents					883:892	many efficient adsorbents	868:892	many efficient adsorbents	868:892	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	7	42	theme	14.3 mg/g	1013:1021	arg1	0.83 mg/L					993:1001	0.83 mg/L	993:1001	0.83 mg/L	993:1001	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	7	42	theme	14.3 mg/g	1013:1021	arg1	qe					1007:1008	qe	1007:1008	qe	1007:1008	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	10	43	theme	lower	1252:1256	arg1	temperature					1258:1268	A lower temperature	1250:1268	A lower temperature	1250:1268	A lower temperature and higher pH slightly increased the adsorption capacity of GCCSC beads.
31593834	3	44	theme	Cu2+	508:511	arg1	immobilization					490:503	the immobilization	486:503	the immobilization of Cu2+ from both water and soil	486:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	8	45	theme	26.0 mg/L	1067:1075	arg1	Ce					1061:1062	Ce	1061:1062	Ce of 26.0 mg/L and qe of 22.4 mg/g	1061:1095	The PC decreased to 0.055 mg/g/μM at Ce of 26.0 mg/L and qe of 22.4 mg/g.
31593834	12	46	theme	direct	1444:1449	arg1	contact					1451:1457	the direct contact	1440:1457	the direct contact between soil and the graphene surface	1440:1495	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	2	47	theme	adsorbents	269:278	arg1	low					283:285	low	283:285	low	283:285	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	2	47	theme	adsorbents	269:278	arg1	strength					243:250	the mechanical strength	228:250	the mechanical strength of most graphene adsorbents	228:278	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	0	48	theme	water	48:52	arg1	decontamination					11:25	Adsorptive decontamination	0:25	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene	0:86	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	12	49	theme	Cu2+	1511:1514	arg1	efficiency					1531:1540	a high Cu2+ immobilization efficiency	1504:1540	a high Cu2+ immobilization efficiency	1504:1540	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	2	50	theme	graphene	260:267	arg1	adsorbents					269:278	most graphene adsorbents	255:278	most graphene adsorbents	255:278	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	9	51	theme	Cu2+	1125:1128	arg1	fast					1161:1164	fast	1161:1164	fast	1161:1164	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	9	51	theme	Cu2+	1125:1128	arg1	kinetics					1113:1120	The adsorption kinetics	1098:1120	The adsorption kinetics of Cu2+ on GCCSC beads	1098:1143	The adsorption kinetics of Cu2+ on GCCSC beads were moderately fast and required approximately 3 h to reach equilibrium with a k2 of 0.0021 g/(mg·min).
31593834	12	52	theme	high	1506:1509	arg1	efficiency					1531:1540	a high Cu2+ immobilization efficiency	1504:1540	a high Cu2+ immobilization efficiency	1504:1540	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	8	53	theme	qe	1081:1082	arg1	Ce					1061:1062	Ce	1061:1062	Ce of 26.0 mg/L and qe of 22.4 mg/g	1061:1095	The PC decreased to 0.055 mg/g/μM at Ce of 26.0 mg/L and qe of 22.4 mg/g.
31593834	2	54	theme	most	255:258	arg1	adsorbents					269:278	most graphene adsorbents	255:278	most graphene adsorbents	255:278	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	6	55	theme	beads	760:764	arg1	22.4 mg/g					770:778	22.4 mg/g	770:778	22.4 mg/g in aqueous systems	770:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	6	55	theme	beads	760:764	arg1	capacity					742:749	The Cu2+ adsorption capacity	722:749	The Cu2+ adsorption capacity of GCCSC beads	722:764	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	3	56	theme	composite	436:444	arg1	beads					446:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads	381:450	carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil	381:536	In this study, we prepared carboxylated graphene oxide/chitosan/cellulose (GCCSC) composite beads with good mechanical strength for the immobilization of Cu2+ from both water and soil.
31593834	4	57	theme	beads	571:575	arg1	3.2 N					581:585	3.2 N	581:585	3.2 N	581:585	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	57	theme	beads	571:575	arg1	limit					556:560	The proportional limit	539:560	The proportional limit of GCCSC beads	539:575	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	12	58	theme	beads	1426:1430	arg1	structure					1407:1415	The porous structure	1396:1415	The porous structure of GCCSC beads	1396:1430	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	12	59	theme	GCCSC	1420:1424	arg1	beads					1426:1430	GCCSC beads	1420:1430	GCCSC beads	1420:1430	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	6	60	theme	GCCSC	754:758	arg1	beads					760:764	GCCSC beads	754:764	GCCSC beads	754:764	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	6	61	theme	efficient	873:881	arg1	adsorbents					883:892	many efficient adsorbents	868:892	many efficient adsorbents	868:892	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	4	62	theme	GCCSC	565:569	arg1	beads					571:575	GCCSC beads	565:575	GCCSC beads	565:575	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	8	63	theme	22.4 mg/g	1087:1095	arg1	qe					1081:1082	qe	1081:1082	qe	1081:1082	The PC decreased to 0.055 mg/g/μM at Ce of 26.0 mg/L and qe of 22.4 mg/g.
31593834	8	63	theme	22.4 mg/g	1087:1095	arg1	26.0 mg/L					1067:1075	26.0 mg/L	1067:1075	26.0 mg/L	1067:1075	The PC decreased to 0.055 mg/g/μM at Ce of 26.0 mg/L and qe of 22.4 mg/g.
31593834	2	64	theme	mechanical	232:241	arg1	low					283:285	low	283:285	low	283:285	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	2	64	theme	mechanical	232:241	arg1	strength					243:250	the mechanical strength	228:250	the mechanical strength of most graphene adsorbents	228:278	However, the mechanical strength of most graphene adsorbents is low and the fragile graphene sheets are released into the environment.
31593834	6	65	theme	Cu2+	810:813	arg1	concentration					815:827	initial Cu2+ concentration	802:827	initial Cu2+ concentration	802:827	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	10	66	theme	adsorption	1307:1316	arg1	capacity					1318:1325	the adsorption capacity	1303:1325	the adsorption capacity of GCCSC beads	1303:1340	A lower temperature and higher pH slightly increased the adsorption capacity of GCCSC beads.
31593834	4	67	theme	oxide	622:626	arg1	0.2 N					635:639	0.2 N	635:639	0.2 N	635:639	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	67	theme	oxide	622:626	arg1	beads					628:632	graphene oxide beads	613:632	graphene oxide beads (0.2 N)	613:640	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	5	68	theme	brittle	696:702	arg1	failure					704:710	brittle failure	696:710	brittle failure	696:710	The largest pressure for GCCSC beads recorded before brittle failure was 26 N.
31593834	6	69	theme	40 μg/mL	832:839	arg1	concentration					815:827	initial Cu2+ concentration	802:827	initial Cu2+ concentration	802:827	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	13	70	dep	water	1663:1667	arg1	remediation					1678:1688	remediation	1678:1688	remediation	1678:1688	The implications for the design of high-performance graphene adsorbents for water and soil remediation are discussed.
31593834	7	71	theme	partition	899:907	arg1	coefficient					909:919	The partition coefficient	895:919	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads	895:965	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	7	71	theme	partition	899:907	arg1	PC					922:923	PC	922:923	PC	922:923	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	4	72	theme	graphene	613:620	arg1	0.2 N					635:639	0.2 N	635:639	0.2 N	635:639	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	4	72	theme	graphene	613:620	arg1	beads					628:632	graphene oxide beads	613:632	graphene oxide beads (0.2 N)	613:640	The proportional limit of GCCSC beads was 3.2 N, a much larger value than graphene oxide beads (0.2 N).
31593834	7	73	theme	0.83 mg/L	993:1001	arg1	Ce					987:988	Ce	987:988	Ce of 0.83 mg/L and qe of 14.3 mg/g	987:1021	The partition coefficient (PC) for the Cu2+ adsorption onto GCCSC beads was 1.12 mg/g/μM at Ce of 0.83 mg/L and qe of 14.3 mg/g.
31593834	12	74	theme	graphene	1480:1487	arg1	surface					1489:1495	the graphene surface	1476:1495	the graphene surface	1476:1495	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	6	75	theme	adsorption	731:740	arg1	22.4 mg/g					770:778	22.4 mg/g	770:778	22.4 mg/g in aqueous systems	770:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	6	75	theme	adsorption	731:740	arg1	capacity					742:749	The Cu2+ adsorption capacity	722:749	The Cu2+ adsorption capacity of GCCSC beads	722:764	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	10	76	theme	beads	1336:1340	arg1	capacity					1318:1325	the adsorption capacity	1303:1325	the adsorption capacity of GCCSC beads	1303:1340	A lower temperature and higher pH slightly increased the adsorption capacity of GCCSC beads.
31593834	0	77	theme	carboxylated	66:77	arg1	graphene					79:86	carboxylated graphene	66:86	carboxylated graphene	66:86	Adsorptive decontamination of Cu2+-contaminated water and soil by carboxylated graphene oxide/chitosan/cellulose composite beads.
31593834	12	78	theme	porous	1400:1405	arg1	structure					1407:1415	The porous structure	1396:1415	The porous structure of GCCSC beads	1396:1430	The porous structure of GCCSC beads blocked the direct contact between soil and the graphene surface; thus, a high Cu2+ immobilization efficiency was achieved by GCCSC beads applied to soil.
31593834	6	79	theme	Cu2+	726:729	arg1	22.4 mg/g					770:778	22.4 mg/g	770:778	22.4 mg/g in aqueous systems	770:797	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	6	79	theme	Cu2+	726:729	arg1	capacity					742:749	The Cu2+ adsorption capacity	722:749	The Cu2+ adsorption capacity of GCCSC beads	722:764	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31593834	1	80	theme	diverse	178:184	arg1	pollutants					186:195	diverse pollutants	178:195	diverse pollutants from aqueous systems	178:216	Graphene adsorbents have been applied to remove diverse pollutants from aqueous systems.
31593834	6	81	with	competitive	851:861	arg1	adsorbents					883:892	many efficient adsorbents	868:892	many efficient adsorbents	868:892	The Cu2+ adsorption capacity of GCCSC beads was 22.4 mg/g in aqueous systems at initial Cu2+ concentration of 40 μg/mL, which is competitive with many efficient adsorbents.
31561056	4	0	theme	controlled	868:877	arg1	degradation					879:889	controlled degradation	868:889	controlled degradation	868:889	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	1	1	theme	high	199:202	arg1	compatibility					204:216	their high compatibility	193:216	their high compatibility	193:216	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	3	2	theme	fluorescent	576:586	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	2	3	dep	self-healing	388:399	arg1	i.e.					383:386	i.e.	383:386	i.e.	383:386	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	2	4	theme	tissues	374:380	arg1	properties					360:369	the intrinsic properties	346:369	the intrinsic properties of tissues, i.e. self-healing and swelling	346:412	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	1	5	theme	Biodegradable	84:96	arg1	hydrogels					98:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	5	theme	Biodegradable	84:96	arg1	materials					148:156	promising extracellular matrix-like materials	112:156	promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion	112:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	3	6	theme	dimensional	559:569	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	7	theme	natural	546:552	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	4	8	theme	developed	737:745	arg1	heterostructure					786:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	0	9	theme	biomedical	59:68	arg1	applications					70:81	biomedical applications	59:81	biomedical applications	59:81	Sweet-MXene hydrogel with mixed-dimensional components for biomedical applications.
31561056	3	10	theme	electrically	439:450	arg1	hydrogel					485:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel	432:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks	432:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	11	theme	novel	499:503	arg1	compositions					523:534	novel mixed-dimensional compositions	499:534	novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots	499:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	11	theme	novel	499:503	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	12	theme	facile	432:437	arg1	hydrogel					485:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel	432:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks	432:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	1	13	theme	promising	112:120	arg1	hydrogels					98:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	13	theme	promising	112:120	arg1	materials					148:156	promising extracellular matrix-like materials	112:156	promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion	112:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	0	14	theme	Sweet-MXene	0:10	arg1	hydrogel					12:19	Sweet-MXene hydrogel	0:19	Sweet-MXene hydrogel with mixed-dimensional components for biomedical applications	0:81	Sweet-MXene hydrogel with mixed-dimensional components for biomedical applications.
31561056	3	15	theme	fluorescent	625:635	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	1	16	theme	extracellular	122:134	arg1	hydrogels					98:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	16	theme	extracellular	122:134	arg1	materials					148:156	promising extracellular matrix-like materials	112:156	promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion	112:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	17	theme	administration	227:240	arg1	invasion					254:261	minimal invasion	246:261	minimal invasion	246:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	17	theme	administration	227:240	arg1	ease					219:222	ease	219:222	ease of administration	219:240	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	17	theme	administration	227:240	arg1	compatibility					204:216	their high compatibility	193:216	their high compatibility	193:216	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	0	18	theme	mixed-dimensional	26:42	arg1	components					44:53	mixed-dimensional components	26:53	mixed-dimensional components	26:53	Sweet-MXene hydrogel with mixed-dimensional components for biomedical applications.
31561056	4	19	theme	desired	847:853	arg1	swelling					855:862	desired swelling	847:862	desired swelling	847:862	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	1	20	theme	matrix-like	136:146	arg1	hydrogels					98:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels	84:106	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	1	20	theme	matrix-like	136:146	arg1	materials					148:156	promising extracellular matrix-like materials	112:156	promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion	112:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	3	21	link	crosslinked	692:702	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	2	22	theme	intrinsic	350:358	arg1	properties					360:369	the intrinsic properties	346:369	the intrinsic properties of tissues, i.e. self-healing and swelling	346:412	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	1	23	theme	minimal	246:252	arg1	invasion					254:261	minimal invasion	246:261	minimal invasion	246:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	5	24	theme	conductive	910:919	arg1	composite					921:929	This electrically conductive composite	892:929	This electrically conductive composite	892:929	This electrically conductive composite is highly biocompatible, it supported cell attachment and survival.
31561056	3	25	dep	honey	603:607	arg1	delaminated					610:620	delaminated	610:620	delaminated	610:620	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	4	26	theme	versatile	747:755	arg1	heterostructure					786:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	3	27	theme	mixed-dimensional	505:521	arg1	compositions					523:534	novel mixed-dimensional compositions	499:534	novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots	499:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	27	theme	mixed-dimensional	505:521	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	28	theme	S-MXene	476:482	arg1	hydrogel					485:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel	432:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks	432:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	29	theme	polymeric	704:712	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	2	30	dep	hydrogels	279:287	arg1	considered					295:304	considered	295:304	to be considered for regenerative therapies	289:331	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	3	31	theme	conductive	452:461	arg1	hydrogel					485:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel	432:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks	432:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	32	theme	chitosan	714:721	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	2	33	theme	injectable	268:277	arg1	hydrogels					279:287	The injectable hydrogels	264:287	The injectable hydrogels to be considered for regenerative therapies	264:331	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	4	34	theme	porous	822:827	arg1	architecture					829:840	excellent porous architecture	812:840	excellent porous architecture	812:840	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	3	35	theme	sweet-MXene	463:473	arg1	hydrogel					485:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel	432:492	facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks	432:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	36	with	hydrogel	485:492	arg1	compositions					523:534	novel mixed-dimensional compositions	499:534	novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots	499:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	36	with	hydrogel	485:492	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	37	theme	Ti3C2	655:659	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	0	38	with	hydrogel	12:19	arg1	components					44:53	mixed-dimensional components	26:53	mixed-dimensional components	26:53	Sweet-MXene hydrogel with mixed-dimensional components for biomedical applications.
31561056	1	39	theme	biomedical	162:171	arg1	applications					173:184	biomedical applications	162:184	biomedical applications due to their high compatibility, ease of administration and minimal invasion	162:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	3	40	theme	titanium	637:644	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	41	theme	3D	689:690	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	2	42	theme	regenerative	310:321	arg1	therapies					323:331	regenerative therapies	310:331	regenerative therapies	310:331	The injectable hydrogels to be considered for regenerative therapies should mimic the intrinsic properties of tissues, i.e. self-healing and swelling.
31561056	4	43	theme	Ti3C2-MXene-honey-chitosan	758:783	arg1	heterostructure					786:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure	733:800	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31561056	3	44	theme	2D	622:623	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	45	theme	honey	603:607	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	46	theme	crosslinked	692:702	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	1	47	theme	due	186:188	arg1	applications					173:184	biomedical applications	162:184	biomedical applications due to their high compatibility, ease of administration and minimal invasion	162:261	Biodegradable hydrogels are promising extracellular matrix-like materials for biomedical applications due to their high compatibility, ease of administration and minimal invasion.
31561056	5	48	dep	cell	969:972	arg1	attachment					974:983	attachment	974:983	attachment	974:983	This electrically conductive composite is highly biocompatible, it supported cell attachment and survival.
31561056	3	49	from	hydrogel	485:492	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	49	from	hydrogel	485:492	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	50	theme	carbon	588:593	arg1	dots					595:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	natural zero dimensional (0D) fluorescent carbon dots	546:598	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	51	theme	bioinspired	677:687	arg1	networks					723:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	bioinspired 3D crosslinked polymeric chitosan networks	677:730	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	3	52	theme	carbide	646:652	arg1	nanosheets					662:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets	603:671	Here, we present facile electrically conductive sweet-MXene (S-MXene) hydrogel with novel mixed-dimensional compositions including natural zero dimensional (0D) fluorescent carbon dots in honey, delaminated 2D fluorescent titanium carbide (Ti3C2) nanosheets and bioinspired 3D crosslinked polymeric chitosan networks.
31561056	4	53	theme	excellent	812:820	arg1	architecture					829:840	excellent porous architecture	812:840	excellent porous architecture	812:840	The developed versatile (Ti3C2-MXene-honey-chitosan) heterostructure exhibited excellent porous architecture with desired swelling and controlled degradation.
31887878	2	0	theme	urethane	424:431	arg1	linkage					433:439	urethane linkage	424:439	urethane linkage	424:439	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	7	1	contain	had	1252:1254	arg2	activity					1279:1286	the best antibacterial activity	1256:1286	the best antibacterial activity against Escherichia coli and Staphylococcus aureus	1256:1337	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	1	contain	had	1252:1254	arg1	composites					1110:1119	the composites	1106:1119	the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1106:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	2	theme	wound	1409:1413	arg1	dressings					1415:1423	wound dressings	1409:1423	wound dressings	1409:1423	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	4	3	contain	containing	776:785	arg2	CS					787:788	CS	787:788	CS (50 wt%)	787:797	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	4	3	contain	containing	776:785	arg1	composite					766:774	the composite	762:774	the composite containing CS (50 wt%)	762:797	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	4	3	contain	containing	776:785	arg2	%					796:796	50 wt%	791:796	50 wt%	791:796	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	3	4	theme	composites	627:636	arg1	properties					609:618	the antibacterial properties	591:618	the antibacterial properties of the composites	591:636	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	2	5	theme	storage	545:551	arg1	modulus					553:559	storage modulus	545:559	storage modulus	545:559	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	7	6	theme	20	1203:1204	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	1	7	theme	polyvinyl	232:240	arg1	PVP					255:257	PVP	255:257	PVP	255:257	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	7	theme	polyvinyl	232:240	arg1	pyrrolidone					242:252	polyvinyl pyrrolidone	232:252	polyvinyl pyrrolidone (PVP)	232:258	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	0	8	theme	properties	93:102	arg1	evaluation					73:82	evaluation	73:82	evaluation of their properties for using as wound healing dressing	73:138	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	0	8	theme	properties	93:102	arg1	linkage					61:67	urethane cross linkage	46:67	urethane cross linkage	46:67	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	3	9	theme	cell	676:679	arg1	counting					681:688	viable bacterial cell counting	659:688	viable bacterial cell counting	659:688	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	7	10	theme	2 wt	1238:1241	arg1	HMDACS					1244:1249	2 wt% HMDACS	1238:1249	2 wt% HMDACS	1238:1249	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	11	theme	60	1199:1200	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	12	theme	%	1212:1212	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	1	13	theme	biocomposite	159:170	arg1	films					172:176	biocomposite films	159:176	biocomposite films	159:176	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	2	14	theme	mechanical	444:453	arg1	properties					455:464	mechanical properties	444:464	mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ	444:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	14	theme	mechanical	444:453	arg1	elongation					492:501	elongation	492:501	elongation	492:501	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	14	theme	mechanical	444:453	arg1	properties					526:535	dynamic-mechanical properties	507:535	dynamic-mechanical properties such as storage modulus and tan δ	507:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	14	theme	mechanical	444:453	arg1	strength					482:489	tensile strength	474:489	tensile strength	474:489	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	3	15	theme	viable	659:664	arg1	counting					681:688	viable bacterial cell counting	659:688	viable bacterial cell counting	659:688	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	4	16	theme	mechanical	862:871	arg1	properties					873:882	the highest mechanical properties	850:882	the highest mechanical properties	850:882	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	2	17	from	effects	308:314	arg1	properties					455:464	mechanical properties	444:464	mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ	444:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	17	from	effects	308:314	arg1	elongation					492:501	elongation	492:501	elongation	492:501	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	17	from	effects	308:314	arg1	properties					526:535	dynamic-mechanical properties	507:535	dynamic-mechanical properties such as storage modulus and tan δ	507:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	17	from	effects	308:314	arg1	strength					482:489	tensile strength	474:489	tensile strength	474:489	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	7	18	theme	25	1179:1180	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	2	19	theme	cross-linking	383:395	arg1	agent					397:401	cross-linking agent	383:401	cross-linking agent	383:401	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	3	20	theme	bacterial	666:674	arg1	counting					681:688	viable bacterial cell counting	659:688	viable bacterial cell counting	659:688	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	7	21	used	used	1358:1361	arg2	they					1346:1349	they	1346:1349	they	1346:1349	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	21	used	used	1358:1361	arg2	materials					1376:1384	promising materials	1366:1384	promising materials for the preparation of wound dressings	1366:1423	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	2	22	theme	tan	565:567	arg1	δ					569:569	tan δ	565:569	tan δ	565:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	6	23	theme	angle	1024:1028	arg1	values					1030:1035	low contact angle values	1012:1035	low contact angle values	1012:1035	In general, low contact angle values revealed the hydrophilicity of the prepared films.
31887878	7	24	theme	50	1175:1176	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	0	25	theme	composites	30:39	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.	0:139	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	6	26	theme	prepared	1072:1079	arg1	films					1081:1085	the prepared films	1068:1085	the prepared films	1068:1085	In general, low contact angle values revealed the hydrophilicity of the prepared films.
31887878	0	27	theme	healing	123:129	arg1	dressing					131:138	wound healing dressing	117:138	wound healing dressing	117:138	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	6	28	theme	contact	1016:1022	arg1	values					1030:1035	low contact angle values	1012:1035	low contact angle values	1012:1035	In general, low contact angle values revealed the hydrophilicity of the prepared films.
31887878	0	29	theme	chitosan-based	15:28	arg1	composites					30:39	chitosan-based composites	15:39	chitosan-based composites	15:39	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	0	30	theme	wound	117:121	arg1	healing					123:129	wound healing	117:129	wound healing dressing	117:138	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	5	31	theme	uniform	942:948	arg1	distribution					950:961	uniform distribution	942:961	uniform distribution of components	942:975	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	6	32	theme	low	1012:1014	arg1	values					1030:1035	low contact angle values	1012:1035	low contact angle values	1012:1035	In general, low contact angle values revealed the hydrophilicity of the prepared films.
31887878	7	33	theme	dressings	1415:1423	arg1	preparation					1394:1404	the preparation	1390:1404	the preparation of wound dressings	1390:1423	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	0	34	theme	urethane	46:53	arg1	linkage					61:67	urethane cross linkage	46:67	urethane cross linkage	46:67	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	1	35	contain	containing	178:187	arg2	pyrrolidone					242:252	polyvinyl pyrrolidone	232:252	polyvinyl pyrrolidone (PVP)	232:258	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg2	chitosan					189:196	chitosan	189:196	chitosan (CS)	189:201	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg2	alcohol					214:220	polyvinyl alcohol	204:220	polyvinyl alcohol (PVA)	204:226	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg2	CS					199:200	CS	199:200	CS	199:200	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg2	PVA					223:225	PVA	223:225	PVA	223:225	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg1	films					172:176	biocomposite films	159:176	biocomposite films	159:176	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	35	contain	containing	178:187	arg2	PVP					255:257	PVP	255:257	PVP	255:257	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	5	36	theme	Scanning	885:892	arg1	SEM					915:917	SEM	915:917	SEM	915:917	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	5	36	theme	Scanning	885:892	arg1	microscopy					903:912	Scanning electron microscopy	885:912	Scanning electron microscopy (SEM) micrographs	885:930	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	4	37	theme	highest	854:860	arg1	properties					873:882	the highest mechanical properties	850:882	the highest mechanical properties	850:882	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	7	38	theme	%	1242:1242	arg1	HMDACS					1244:1249	2 wt% HMDACS	1238:1249	2 wt% HMDACS	1238:1249	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	2	39	theme	tensile	474:480	arg1	strength					482:489	tensile strength	474:489	tensile strength	474:489	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	5	40	theme	electron	894:901	arg1	SEM					915:917	SEM	915:917	SEM	915:917	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	5	40	theme	electron	894:901	arg1	microscopy					903:912	Scanning electron microscopy	885:912	Scanning electron microscopy (SEM) micrographs	885:930	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	7	41	theme	promising	1366:1374	arg1	materials					1376:1384	promising materials	1366:1384	promising materials for the preparation of wound dressings	1366:1423	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	7	41	theme	promising	1366:1374	arg1	they					1346:1349	they	1346:1349	they	1346:1349	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	1	42	theme	different	266:274	arg1	ratios					276:281	different ratios	266:281	different ratios	266:281	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	5	43	theme	components	966:975	arg1	distribution					950:961	uniform distribution	942:961	uniform distribution of components	942:975	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	5	44	theme	microscopy	903:912	arg1	micrographs					920:930	Scanning electron microscopy (SEM) micrographs	885:930	Scanning electron microscopy (SEM) micrographs	885:930	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	5	45	theme	polymer	984:990	arg1	matrix					992:997	the polymer matrix	980:997	the polymer matrix	980:997	Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix.
31887878	0	46	theme	cross	55:59	arg1	linkage					61:67	urethane cross linkage	46:67	urethane cross linkage	46:67	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	7	47	theme	antibacterial	1265:1277	arg1	activity					1279:1286	the best antibacterial activity	1256:1286	the best antibacterial activity against Escherichia coli and Staphylococcus aureus	1256:1337	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	3	48	theme	bacterial	719:727	arg1	growth					729:734	the bacterial growth	715:734	the bacterial growth	715:734	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	2	49	theme	dynamic-mechanical	507:524	arg1	modulus					553:559	storage modulus	545:559	storage modulus	545:559	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	49	theme	dynamic-mechanical	507:524	arg1	δ					569:569	tan δ	565:569	tan δ	565:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	49	theme	dynamic-mechanical	507:524	arg1	properties					526:535	dynamic-mechanical properties	507:535	dynamic-mechanical properties such as storage modulus and tan δ	507:569	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	7	50	theme	%	1188:1188	arg1	concentration					1158:1170	concentration	1158:1170	concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS,	1158:1250	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	4	51	theme	final	741:745	arg1	results					747:753	The final results	737:753	The final results	737:753	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	0	52	with	Preparation	0:10	arg1	evaluation					73:82	evaluation	73:82	evaluation of their properties for using as wound healing dressing	73:138	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	0	52	with	Preparation	0:10	arg1	linkage					61:67	urethane cross linkage	46:67	urethane cross linkage	46:67	Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing.
31887878	1	53	theme	polyvinyl	204:212	arg1	PVA					223:225	PVA	223:225	PVA	223:225	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	53	theme	polyvinyl	204:212	arg1	alcohol					214:220	polyvinyl alcohol	204:220	polyvinyl alcohol (PVA)	204:226	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	1	54	with	films	172:176	arg1	ratios					276:281	different ratios	266:281	different ratios	266:281	In this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided.
31887878	3	55	theme	antibacterial	595:607	arg1	properties					609:618	the antibacterial properties	591:618	the antibacterial properties of the composites	591:636	Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth.
31887878	6	56	theme	films	1081:1085	arg1	hydrophilicity					1050:1063	the hydrophilicity	1046:1063	the hydrophilicity of the prepared films	1046:1085	In general, low contact angle values revealed the hydrophilicity of the prepared films.
31887878	7	57	theme	best	1260:1263	arg1	activity					1279:1286	the best antibacterial activity	1256:1286	the best antibacterial activity against Escherichia coli and Staphylococcus aureus	1256:1337	It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be used as promising materials for the preparation of wound dressings.
31887878	4	58	dep	showed	755:760	arg1	had					846:848	had	846:848	showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties	755:882	The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties.
31887878	2	59	theme	linkage	433:439	arg1	hexamethylene					326:338	hexamethylene 1	326:340	hexamethylene 1	326:340	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	59	theme	linkage	433:439	arg1	agent					397:401	cross-linking agent	383:401	cross-linking agent	383:401	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
31887878	2	59	theme	linkage	433:439	arg1	formation					411:419	the formation	407:419	the formation of urethane linkage	407:439	The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross-linking agent and the formation of urethane linkage on mechanical properties such as tensile strength, elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied.
30143127	7	0	theme	non-loading	1118:1128	arg1	tissue					1135:1140	non-loading bone tissue	1118:1140	non-loading bone tissue engineering application	1118:1164	Consequently, this study suggested SF/CS/SA scaffolds possess potential in non-loading bone tissue engineering application.
30143127	2	1	from	silicate	342:349	arg1	Ca2+					324:327	Ca2+	324:327	Ca2+ from calcium silicate (CS)	324:354	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	6	2	dep	25/CS	977:981	arg1	scaffolds					993:1001	scaffolds	993:1001	scaffolds	993:1001	Besides, BMSCs cultured with 25/CS and 12/CS scaffolds showed high ALP activity, respectively.
30143127	3	3	theme	in	551:552	arg1	characterizations					532:548	physical characterizations	523:548	physical characterizations	523:548	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	3	3	theme	in	551:552	arg1	cytocompatibility					560:576	in vitro cytocompatibility	551:576	in vitro cytocompatibility	551:576	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	5	4	theme	cells	933:937	arg1	proliferation					896:908	proliferation	896:908	proliferation of bone marrow stromal cells (BMSCs)	896:945	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	5	theme	stromal	925:931	arg1	BMSCs					940:944	BMSCs	940:944	BMSCs	940:944	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	5	theme	stromal	925:931	arg1	cells					933:937	bone marrow stromal cells	913:937	bone marrow stromal cells (BMSCs)	913:945	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	7	6	theme	SF/CS/SA	1078:1085	arg1	scaffolds					1087:1095	SF/CS/SA scaffolds	1078:1095	SF/CS/SA scaffolds	1078:1095	Consequently, this study suggested SF/CS/SA scaffolds possess potential in non-loading bone tissue engineering application.
30143127	4	7	theme	scaffolds	792:800	arg1	bioactivity					761:771	bioactivity	761:771	bioactivity	761:771	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	4	7	theme	scaffolds	792:800	arg1	degradation					724:734	degradation	724:734	degradation	724:734	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	4	7	theme	scaffolds	792:800	arg1	resistance					749:758	compression resistance	737:758	compression resistance	737:758	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	4	7	theme	scaffolds	792:800	arg1	hydrophilicity					708:721	hydrophilicity	708:721	hydrophilicity	708:721	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	4	7	theme	scaffolds	792:800	arg1	pH					777:778	pH	777:778	pH	777:778	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	5	8	theme	marrow	918:923	arg1	BMSCs					940:944	BMSCs	940:944	BMSCs	940:944	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	8	theme	marrow	918:923	arg1	cells					933:937	bone marrow stromal cells	913:937	bone marrow stromal cells (BMSCs)	913:945	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	7	9	theme	bone	1130:1133	arg1	tissue					1135:1140	non-loading bone tissue	1118:1140	non-loading bone tissue engineering application	1118:1164	Consequently, this study suggested SF/CS/SA scaffolds possess potential in non-loading bone tissue engineering application.
30143127	2	10	theme	network	294:300	arg1	hydrogel					302:309	an interpenetrating network hydrogel	274:309	an interpenetrating network hydrogel	274:309	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	0	11	theme	silk/calcium	14:25	arg1	composite					52:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	3	12	theme	alkaline	582:589	arg1	ALP					604:606	ALP	604:606	ALP	604:606	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	3	12	theme	alkaline	582:589	arg1	phosphatase					591:601	alkaline phosphatase	582:601	alkaline phosphatase (ALP) assay	582:613	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	4	13	theme	scaffolds	673:681	arg1	structure					651:659	the porous structure	640:659	the porous structure of SF/CS/SA scaffolds	640:681	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	0	14	theme	Biocompatible	0:12	arg1	composite					52:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	2	15	theme	calcium	334:340	arg1	CS					352:353	CS	352:353	CS	352:353	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	2	15	theme	calcium	334:340	arg1	silicate					342:349	calcium silicate	334:349	calcium silicate (CS)	334:354	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	4	16	theme	SF/CS/SA	664:671	arg1	scaffolds					673:681	SF/CS/SA scaffolds	664:681	SF/CS/SA scaffolds	664:681	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	0	17	theme	alginate	43:50	arg1	composite					52:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	3	18	theme	phosphatase	591:601	arg1	characterizations					532:548	physical characterizations	523:548	physical characterizations	523:548	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	3	18	theme	phosphatase	591:601	arg1	assay					609:613	alkaline phosphatase (ALP) assay	582:613	alkaline phosphatase (ALP) assay	582:613	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	1	19	theme	bone	127:130	arg1	engineering					139:149	bone tissue engineering	127:149	bone tissue engineering	127:149	Scaffolds are crucial for bone tissue engineering since their compositions and properties could significantly affect the seeded cells' behavior.
30143127	5	20	theme	%	849:849	arg1	concentrations					821:834	CS concentrations	818:834	CS concentrations of 25% and 12% (25/CS and 12/CS)	818:867	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	1	21	theme	seeded	222:227	arg1	cells					229:233	the seeded cells	218:233	the seeded cells' behavior	218:243	Scaffolds are crucial for bone tissue engineering since their compositions and properties could significantly affect the seeded cells' behavior.
30143127	4	22	theme	porous	644:649	arg1	structure					651:659	the porous structure	640:659	the porous structure of SF/CS/SA scaffolds	640:681	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	7	23	dep	suggested	1068:1076	arg1	possess					1097:1103	possess	1097:1103	suggested SF/CS/SA scaffolds possess potential in non-loading bone tissue engineering application	1068:1164	Consequently, this study suggested SF/CS/SA scaffolds possess potential in non-loading bone tissue engineering application.
30143127	5	24	with	Scaffolds	803:811	arg1	concentrations					821:834	CS concentrations	818:834	CS concentrations of 25% and 12% (25/CS and 12/CS)	818:867	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	2	25	theme	sodium	406:411	arg1	alginate					413:420	sodium alginate	406:420	sodium alginate (SA)	406:425	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	2	25	theme	sodium	406:411	arg1	SA					423:424	SA	423:424	SA	423:424	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	0	26	theme	bone	76:79	arg1	engineering					88:98	bone tissue engineering	76:98	bone tissue engineering	76:98	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	1	27	theme	tissue	132:137	arg1	engineering					139:149	bone tissue engineering	127:149	bone tissue engineering	127:149	Scaffolds are crucial for bone tissue engineering since their compositions and properties could significantly affect the seeded cells' behavior.
30143127	5	28	theme	CS	818:819	arg1	concentrations					821:834	CS concentrations	818:834	CS concentrations of 25% and 12% (25/CS and 12/CS)	818:867	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	29	theme	bone	913:916	arg1	marrow					918:923	bone marrow	913:923	bone marrow stromal cells (BMSCs)	913:945	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	30	dep	%	849:849	arg1	12/CS					862:866	12/CS	862:866	12/CS	862:866	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	5	30	dep	%	849:849	arg1	25/CS					852:856	25/CS	852:856	25/CS	852:856	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	2	31	theme	interpenetrating	277:292	arg1	hydrogel					302:309	an interpenetrating network hydrogel	274:309	an interpenetrating network hydrogel	274:309	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	3	32	dep	in	551:552	arg1	vitro					554:558	vitro	554:558	vitro	554:558	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	6	33	theme	high	1010:1013	arg1	activity					1019:1026	high ALP activity	1010:1026	high ALP activity	1010:1026	Besides, BMSCs cultured with 25/CS and 12/CS scaffolds showed high ALP activity, respectively.
30143127	4	34	theme	SF/CS/SA	783:790	arg1	scaffolds					792:800	SF/CS/SA scaffolds	783:800	SF/CS/SA scaffolds	783:800	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	5	35	theme	%	841:841	arg1	concentrations					821:834	CS concentrations	818:834	CS concentrations of 25% and 12% (25/CS and 12/CS)	818:867	Scaffolds with CS concentrations of 25% and 12% (25/CS and 12/CS) could dominantly stimulate proliferation of bone marrow stromal cells (BMSCs).
30143127	2	36	theme	silk	384:387	arg1	SF					398:399	SF	398:399	SF	398:399	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	2	36	theme	silk	384:387	arg1	fibroin					389:395	silk fibroin	384:395	silk fibroin (SF)	384:400	In this study, we developed an interpenetrating network hydrogel by utilizing Ca2+ from calcium silicate (CS) to simultaneously crosslink silk fibroin (SF) and sodium alginate (SA).
30143127	4	37	theme	compression	737:747	arg1	resistance					749:758	compression resistance	737:758	compression resistance	737:758	We found that CS inside the porous structure of SF/CS/SA scaffolds could remarkably enhance hydrophilicity, degradation, compression resistance, bioactivity and pH of SF/CS/SA scaffolds.
30143127	6	38	theme	ALP	1015:1017	arg1	activity					1019:1026	high ALP activity	1010:1026	high ALP activity	1010:1026	Besides, BMSCs cultured with 25/CS and 12/CS scaffolds showed high ALP activity, respectively.
30143127	0	39	theme	tissue	81:86	arg1	engineering					88:98	bone tissue engineering	76:98	bone tissue engineering	76:98	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	0	40	theme	silicate/sodium	27:41	arg1	composite					52:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite	0:60	Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering.
30143127	3	41	theme	physical	523:530	arg1	characterizations					532:548	physical characterizations	523:548	physical characterizations	523:548	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	3	41	theme	physical	523:530	arg1	assay					609:613	alkaline phosphatase (ALP) assay	582:613	alkaline phosphatase (ALP) assay	582:613	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
30143127	3	41	theme	physical	523:530	arg1	cytocompatibility					560:576	in vitro cytocompatibility	551:576	in vitro cytocompatibility	551:576	Afterwards, the hydrogels were lyophilized to obtain scaffolds and systematically evaluated by physical characterizations, in vitro cytocompatibility and alkaline phosphatase (ALP) assay.
32278244	0	0	theme	galactooligosaccharides	80:102	arg1	production					66:75	the ultrasound-assisted enzymatic production	32:75	the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid	32:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	2	1	theme	oxidase	434:440	arg1	activation					448:457	glucose oxidase (Gox) activation	426:457	glucose oxidase (Gox) activation during gluconic acid (GA) production	426:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	2	theme	salt	915:918	arg1	composition					920:930	salt composition	915:930	salt composition being the most influential factor	915:964	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	2	theme	salt	915:918	arg1	cheese					884:889	cheese	884:889	cheese whey and whey permeate	884:912	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	1	3	from	problems	161:168	arg1	GOS					208:210	GOS	208:210	GOS	208:210	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	3	from	problems	161:168	arg1	production					196:205	galactooligosaccharide production	173:205	galactooligosaccharide production (GOS) via tranglycosylation of lactose	173:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	2	4	theme	glucose	426:432	arg1	activation					448:457	glucose oxidase (Gox) activation	426:457	glucose oxidase (Gox) activation during gluconic acid (GA) production	426:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	1	5	gly	tranglycosylation	217:233	arg1	lactose					238:244	lactose	238:244	lactose	238:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	5	6	theme	glycaemic	1338:1346	arg1	index					1348:1352	low glycaemic index	1334:1352	low glycaemic index	1334:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	5	7	theme	mixtures	1322:1329	arg1	obtainment					1298:1307	the obtainment	1294:1307	the obtainment of prebiotic mixtures of low glycaemic index	1294:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	0	8	theme	gluconic	108:115	arg1	acid					117:120	gluconic acid	108:120	gluconic acid	108:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	0	9	from	Effect	0:5	arg1	production					66:75	the ultrasound-assisted enzymatic production	32:75	the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid	32:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	5	10	theme	low	1334:1336	arg1	index					1348:1352	low glycaemic index	1334:1352	low glycaemic index	1334:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	2	11	dep	whey	741:744	arg1	permeate					746:753	permeate	746:753	permeate	746:753	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	4	12	theme	multienzymatic	1107:1120	arg1	systems					1122:1128	multienzymatic systems	1107:1128	multienzymatic systems	1107:1128	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	2	13	theme	lactose	723:729	arg1	substrate					712:720	substrate	712:720	substrate (lactose solution, whey permeate, cheese whey)	712:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	13	theme	lactose	723:729	arg1	solution					731:738	lactose solution	723:738	lactose solution	723:738	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	14	theme	lactose	832:838	arg1	solution					840:847	lactose solution	832:847	lactose solution	832:847	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	2	15	theme	glucose	566:572	arg1	oxidation					553:561	the selective oxidation	539:561	the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	539:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	16	theme	matrix	799:804	arg1	influence					786:794	the influence	782:794	the influence of matrix on GOS and GA production	782:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	17	theme	influence	786:794	arg1	terms					773:777	terms	773:777	terms of the influence of matrix on GOS and GA production	773:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	4	18	theme	GOS	989:991	arg1	production					993:1002	GOS production	989:1002	GOS production with the lowest glucose concentration	989:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	19	theme	%	1163:1163	arg1	ultrasound					1149:1158	ultrasound	1149:1158	ultrasound (30% amplitude)	1149:1174	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	19	theme	%	1163:1163	arg1	amplitude					1165:1173	30% amplitude	1161:1173	30% amplitude	1161:1173	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	20	from	solution	1095:1102	arg1	systems					1122:1128	multienzymatic systems	1107:1128	multienzymatic systems	1107:1128	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	1	21	theme	main	156:159	arg1	problems					161:168	the main problems	152:168	the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose	152:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	0	22	theme	acid	117:120	arg1	production					66:75	the ultrasound-assisted enzymatic production	32:75	the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid	32:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	1	23	theme	glucose	350:356	arg1	case					342:345	the case	338:345	the case of glucose	338:356	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	2	24	theme	selective	543:551	arg1	oxidation					553:561	the selective oxidation	539:561	the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	539:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	25	from	terms	773:777	arg1	GOS					809:811	GOS	809:811	GOS	809:811	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	25	from	terms	773:777	arg1	production					820:829	GA production	817:829	GA production	817:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	4	26	theme	production	993:1002	arg1	production					1057:1066	highest GA production	1046:1066	highest GA production	1046:1066	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	26	theme	production	993:1002	arg1	yields					979:984	The highest yields	967:984	The highest yields of GOS production with the lowest glucose concentration	967:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	1	27	theme	problems	161:168	arg1	one					145:147	one	145:147	one	145:147	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	27	theme	problems	161:168	arg1	presence					253:260	the presence	249:260	the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose	249:356	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	27	theme	problems	161:168	arg1	problems					161:168	the main problems	152:168	the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose	152:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	2	28	theme	ultrasound	407:416	arg1	effect					397:402	the effect	393:402	the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production	393:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	29	dep	ultrasound	407:416	arg1	US					419:420	US	419:420	US	419:420	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	30	theme	multienzymatic	594:607	arg1	Gox					648:650	Gox	648:650	Gox	648:650	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	30	theme	multienzymatic	594:607	arg1	β-galactosidase					620:634	β-galactosidase	620:634	β-galactosidase (β-gal)	620:642	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	30	theme	multienzymatic	594:607	arg1	reactions					609:617	multienzymatic reactions	594:617	multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	594:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	31	theme	assisted	653:660	arg1	Gox					648:650	Gox	648:650	Gox	648:650	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	31	theme	assisted	653:660	arg1	β-galactosidase					620:634	β-galactosidase	620:634	β-galactosidase (β-gal)	620:642	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	31	theme	assisted	653:660	arg1	reactions					609:617	multienzymatic reactions	594:617	multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	594:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	1	32	theme	monosaccharides	265:279	arg1	one					145:147	one	145:147	one	145:147	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	32	theme	monosaccharides	265:279	arg1	presence					253:260	the presence	249:260	the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose	249:356	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	32	theme	monosaccharides	265:279	arg1	problems					161:168	the main problems	152:168	the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose	152:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	5	33	theme	prebiotic	1312:1320	arg1	mixtures					1322:1329	prebiotic mixtures	1312:1329	prebiotic mixtures of low glycaemic index	1312:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	4	34	with	treatment	1208:1216	arg1	β-gal					1223:1227	β-gal	1223:1227	β-gal	1223:1227	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	1	35	theme	galactooligosaccharide	173:194	arg1	GOS					208:210	GOS	208:210	GOS	208:210	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	1	35	theme	galactooligosaccharide	173:194	arg1	production					196:205	galactooligosaccharide production	173:205	galactooligosaccharide production (GOS) via tranglycosylation of lactose	173:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	0	36	theme	lactose	14:20	arg1	source					22:27	the lactose source	10:27	the lactose source	10:27	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	4	37	with	production	993:1002	arg1	concentration					1028:1040	the lowest glucose concentration	1009:1040	the lowest glucose concentration	1009:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	3	38	theme	whey	891:894	arg1	composition					920:930	salt composition	915:930	salt composition being the most influential factor	915:964	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	38	theme	whey	891:894	arg1	cheese					884:889	cheese	884:889	cheese whey and whey permeate	884:912	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	2	39	theme	gluconic	577:584	arg1	acid					586:589	gluconic acid	577:589	gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	577:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	4	40	theme	lowest	1013:1018	arg1	concentration					1028:1040	the lowest glucose concentration	1009:1040	the lowest glucose concentration	1009:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	2	41	theme	GA	481:482	arg1	production					485:494	gluconic acid (GA) production	466:494	gluconic acid (GA) production	466:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	4	42	theme	ultrasound	1149:1158	arg1	presence					1137:1144	the presence	1133:1144	the presence of ultrasound (30% amplitude)	1133:1174	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	2	43	theme	different	680:688	arg1	sources					690:696	different sources	680:696	different sources of lactose	680:707	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	44	from	production	820:829	arg1	terms					773:777	terms	773:777	terms of the influence of matrix on GOS and GA production	773:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	2	45	dep	power	665:669	arg1	US					671:672	US	671:672	US	671:672	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	0	46	theme	source	22:27	arg1	Effect					0:5	Effect	0:5	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.	0:121	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	2	47	theme	acid	475:478	arg1	production					485:494	gluconic acid (GA) production	466:494	gluconic acid (GA) production	466:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	4	48	theme	highest	971:977	arg1	yields					979:984	The highest yields	967:984	The highest yields of GOS production with the lowest glucose concentration	967:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	0	49	theme	ultrasound-assisted	36:54	arg1	production					66:75	the ultrasound-assisted enzymatic production	32:75	the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid	32:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	2	50	theme	gluconic	466:473	arg1	production					485:494	gluconic acid (GA) production	466:494	gluconic acid (GA) production	466:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	5	51	theme	index	1348:1352	arg1	mixtures					1322:1329	prebiotic mixtures	1312:1329	prebiotic mixtures of low glycaemic index	1312:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	3	52	from	GOS	809:811	arg1	terms					773:777	terms	773:777	terms of the influence of matrix on GOS and GA production	773:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	2	53	dep	solution	731:738	arg1	whey					763:766	cheese whey	756:766	cheese whey	756:766	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	53	dep	solution	731:738	arg1	whey					741:744	whey	741:744	whey permeate	741:753	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	1	54	theme	glycaemic	315:323	arg1	index					325:329	the glycaemic index	311:329	the glycaemic index	311:329	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
32278244	3	55	theme	whey	900:903	arg1	composition					920:930	salt composition	915:930	salt composition being the most influential factor	915:964	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	55	theme	whey	900:903	arg1	cheese					884:889	cheese	884:889	cheese whey and whey permeate	884:912	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	56	theme	influential	947:957	arg1	factor					959:964	the most influential factor	938:964	the most influential factor	938:964	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	57	theme	GA	817:818	arg1	production					820:829	GA production	817:829	GA production	817:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	4	58	theme	treatment	1208:1216	arg1	1 h					1201:1203	1 h	1201:1203	1 h of treatment with β-gal	1201:1227	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	59	theme	lactose	1087:1093	arg1	solution					1095:1102	lactose solution	1087:1102	lactose solution in multienzymatic systems	1087:1128	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	60	theme	GA	1054:1055	arg1	production					1057:1066	highest GA production	1046:1066	highest GA production	1046:1066	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	4	61	theme	highest	1046:1052	arg1	production					1057:1066	highest GA production	1046:1066	highest GA production	1046:1066	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	5	62	theme	US	1268:1269	arg1	ability					1257:1263	the ability	1253:1263	the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index	1253:1352	This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index.
32278244	2	63	dep	reactions	609:617	arg1	β-gal					637:641	β-gal	637:641	β-gal	637:641	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	63	dep	reactions	609:617	arg1	Gox					648:650	Gox	648:650	Gox	648:650	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	63	dep	reactions	609:617	arg1	β-galactosidase					620:634	β-galactosidase	620:634	β-galactosidase (β-gal)	620:642	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	63	dep	reactions	609:617	arg1	reactions					609:617	multienzymatic reactions	594:617	multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	594:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	64	theme	cheese	756:761	arg1	whey					763:766	cheese whey	756:766	cheese whey	756:766	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	65	theme	Gox	443:445	arg1	activation					448:457	glucose oxidase (Gox) activation	426:457	glucose oxidase (Gox) activation during gluconic acid (GA) production	426:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	3	66	dep	whey	900:903	arg1	permeate					905:912	permeate	905:912	permeate	905:912	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	67	from	influence	786:794	arg1	GOS					809:811	GOS	809:811	GOS	809:811	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	67	from	influence	786:794	arg1	production					820:829	GA production	817:829	GA production	817:829	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	3	68	theme	best	858:861	arg1	results					863:869	the best results	854:869	the best results	854:869	In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor.
32278244	2	69	theme	lactose	701:707	arg1	sources					690:696	different sources	680:696	different sources of lactose	680:707	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	0	70	theme	enzymatic	56:64	arg1	production					66:75	the ultrasound-assisted enzymatic production	32:75	the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid	32:120	Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid.
32278244	2	71	from	effect	397:402	arg1	activation					448:457	glucose oxidase (Gox) activation	426:457	glucose oxidase (Gox) activation during gluconic acid (GA) production	426:494	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	4	72	theme	glucose	1020:1026	arg1	concentration					1028:1040	the lowest glucose concentration	1009:1040	the lowest glucose concentration	1009:1040	The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal.
32278244	2	73	from	acid	586:589	arg1	Gox					648:650	Gox	648:650	Gox	648:650	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	73	from	acid	586:589	arg1	β-galactosidase					620:634	β-galactosidase	620:634	β-galactosidase (β-gal)	620:642	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	2	73	from	acid	586:589	arg1	reactions					609:617	multienzymatic reactions	594:617	multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey)	594:767	In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey).
32278244	1	74	theme	lactose	238:244	arg1	tranglycosylation					217:233	tranglycosylation	217:233	tranglycosylation of lactose	217:244	It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose.
29355454	0	0	theme	sucrose	109:115	arg1	co-substrates					92:104	co-substrates	92:104	co-substrates of sucrose and inulin from Jerusalem artichoke	92:151	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	8	1	theme	bioethanol	1408:1417	arg1	production					1419:1428	bioethanol production	1408:1428	bioethanol production	1408:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	7	2	theme	Total	1203:1207	arg1	yield					1220:1224	Total conversion yield	1203:1224	Total conversion yield	1203:1224	Total conversion yield was approximately 67%.
29355454	1	3	theme	various	194:200	arg1	materials					208:216	various plant materials	194:216	various plant materials	194:216	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	3	4	from	sucrose	589:595	arg1	Inulin					544:549	Inulin	544:549	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g)	544:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	4	from	sucrose	589:595	arg1	parameters					680:689	variable parameters	671:689	variable parameters for optimization	671:706	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	0	5	theme	inulin	121:126	arg1	co-substrates					92:104	co-substrates	92:104	co-substrates of sucrose and inulin from Jerusalem artichoke	92:151	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	4	6	theme	sucrose	750:756	arg1	application					735:745	the application	731:745	the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis	731:858	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	4	6	theme	sucrose	750:756	arg1	viable					863:868	viable	863:868	viable	863:868	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	8	7	theme	oligosaccharides	1304:1319	arg1	substrate					1377:1385	a substrate	1375:1385	a substrate in prebiotics and/or bioethanol production	1375:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	8	7	theme	oligosaccharides	1304:1319	arg1	production					1290:1299	the high value-added production	1269:1299	the high value-added production	1269:1299	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	7	8	theme	conversion	1209:1218	arg1	yield					1220:1224	Total conversion yield	1203:1224	Total conversion yield	1203:1224	Total conversion yield was approximately 67%.
29355454	4	9	theme	inulin	762:767	arg1	application					735:745	the application	731:745	the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis	731:858	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	4	9	theme	inulin	762:767	arg1	viable					863:868	viable	863:868	viable	863:868	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	8	10	from	substrate	1377:1385	arg1	prebiotics					1390:1399	prebiotics	1390:1399	prebiotics	1390:1399	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	8	10	from	substrate	1377:1385	arg1	production					1419:1428	bioethanol production	1408:1428	bioethanol production	1408:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	5	11	theme	9 hr	1073:1076	arg1	period					1063:1068	an incubation period	1049:1068	an incubation period of 9 hr	1049:1076	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	2	12	theme	inulo-oligosaccharide	318:338	arg1	production					271:280	The production	267:280	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS)	267:344	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	3	13	from	niger	642:646	arg1	inulinase					615:623	inulinase	615:623	inulinase from Aspergillus niger (2-7 U/g)	615:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	13	from	niger	642:646	arg1	U/g					653:655	2-7 U/g	649:655	2-7 U/g	649:655	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	13	from	niger	642:646	arg1	w/v					583:585	5-15% w/v	577:585	5-15% w/v	577:585	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	13	from	niger	642:646	arg1	w/v					605:607	50-70% w/v	598:607	50-70% w/v	598:607	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	13	from	niger	642:646	arg1	artichoke					566:574	Jerusalem artichoke	556:574	Jerusalem artichoke (5-15% w/v)	556:586	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	13	from	niger	642:646	arg1	sucrose					589:595	sucrose	589:595	sucrose (50-70% w/v)	589:608	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	1	14	theme	plant	202:206	arg1	materials					208:216	various plant materials	194:216	various plant materials	194:216	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	5	15	theme	Maximum	923:929	arg1	yields					931:936	Maximum yields	923:936	Maximum yields (674.82 mg/g substrate)	923:960	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	15	theme	Maximum	923:929	arg1	substrate					951:959	674.82 mg/g substrate	939:959	674.82 mg/g substrate	939:959	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	%					1003:1003	59.87%	998:1003	59.87% of sucrose	998:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	U/g					1026:1028	5.68 U/g	1021:1028	5.68 U/g of inulinase	1021:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	inulinase					1033:1041	inulinase	1033:1041	inulinase	1033:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	%					985:985	5.95%	981:985	5.95% of inulin	981:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	sucrose					1008:1014	sucrose	1008:1014	sucrose	1008:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	16	theme	inulin	990:995	arg1	inulin					990:995	inulin	990:995	inulin	990:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	8	17	theme	value-added	1278:1288	arg1	substrate					1377:1385	a substrate	1375:1385	a substrate in prebiotics and/or bioethanol production	1375:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	8	17	theme	value-added	1278:1288	arg1	production					1290:1299	the high value-added production	1269:1299	the high value-added production	1269:1299	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	2	18	theme	oligosaccharide	408:422	arg1	yields					424:429	oligosaccharide yields	408:429	oligosaccharide yields	408:429	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	0	19	theme	enzymatic	31:39	arg1	fructo-					41:47	simultaneously enzymatic fructo-	16:47	simultaneously enzymatic fructo-	16:47	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	4	20	theme	inulinase	825:833	arg1	hydrolysis					835:844	inulinase hydrolysis	825:844	inulinase hydrolysis	825:844	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	3	21	from	artichoke	566:574	arg1	Inulin					544:549	Inulin	544:549	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g)	544:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	21	from	artichoke	566:574	arg1	parameters					680:689	variable parameters	671:689	variable parameters for optimization	671:706	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	8	22	used	used	1367:1370	arg2	production					1290:1299	the high value-added production	1269:1299	the high value-added production	1269:1299	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	8	22	used	used	1367:1370	arg2	substrate					1377:1385	a substrate	1375:1385	a substrate in prebiotics and/or bioethanol production	1375:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	3	23	used	used	663:666	arg2	Inulin					544:549	Inulin	544:549	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g)	544:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	23	used	used	663:666	arg2	parameters					680:689	variable parameters	671:689	variable parameters for optimization	671:706	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	24	theme	%	603:603	arg1	sucrose					589:595	sucrose	589:595	sucrose (50-70% w/v)	589:608	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	24	theme	%	603:603	arg1	w/v					605:607	50-70% w/v	598:607	50-70% w/v	598:607	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	25	from	inulinase	615:623	arg1	Inulin					544:549	Inulin	544:549	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g)	544:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	25	from	inulinase	615:623	arg1	parameters					680:689	variable parameters	671:689	variable parameters for optimization	671:706	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	2	26	theme	composite	460:468	arg1	design					470:475	central composite design	452:475	central composite design	452:475	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	6	27	from	co-substrates	1112:1124	arg1	reaction					1133:1140	the reaction	1129:1140	the reaction	1129:1140	The use of sucrose and inulin as co-substrates in the reaction simultaneously produced FOS and IOS from sucrose and inulin.
29355454	1	28	theme	different	245:253	arg1	substrates					255:264	different substrates	245:264	different substrates	245:264	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	5	29	theme	sucrose	1008:1014	arg1	%					1003:1003	59.87%	998:1003	59.87% of sucrose	998:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	29	theme	sucrose	1008:1014	arg1	U/g					1026:1028	5.68 U/g	1021:1028	5.68 U/g of inulinase	1021:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	29	theme	sucrose	1008:1014	arg1	inulinase					1033:1041	inulinase	1033:1041	inulinase	1033:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	29	theme	sucrose	1008:1014	arg1	%					985:985	5.95%	981:985	5.95% of inulin	981:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	29	theme	sucrose	1008:1014	arg1	sucrose					1008:1014	sucrose	1008:1014	sucrose	1008:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	29	theme	sucrose	1008:1014	arg1	inulin					990:995	inulin	990:995	inulin	990:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	1	30	theme	enzymatic	221:229	arg1	hydrolysis					231:240	enzymatic hydrolysis	221:240	enzymatic hydrolysis of different substrates	221:264	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	2	31	theme	central	452:458	arg1	design					470:475	central composite design	452:475	central composite design	452:475	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	0	32	theme	fructo-	41:47	arg1	Optimization					0:11	Optimization	0:11	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.	0:152	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	5	33	theme	incubation	1052:1061	arg1	period					1063:1068	an incubation period	1049:1068	an incubation period of 9 hr	1049:1076	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	1	34	theme	substrates	255:264	arg1	materials					208:216	various plant materials	194:216	various plant materials	194:216	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	1	34	theme	substrates	255:264	arg1	hydrolysis					231:240	enzymatic hydrolysis	221:240	enzymatic hydrolysis of different substrates	221:264	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	0	35	theme	production	75:84	arg1	Optimization					0:11	Optimization	0:11	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.	0:152	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	3	36	theme	Jerusalem	556:564	arg1	artichoke					566:574	Jerusalem artichoke	556:574	Jerusalem artichoke (5-15% w/v)	556:586	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	36	theme	Jerusalem	556:564	arg1	w/v					583:585	5-15% w/v	577:585	5-15% w/v	577:585	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	0	37	theme	inulo-oligosaccharide	53:73	arg1	production					75:84	inulo-oligosaccharide production	53:84	inulo-oligosaccharide production	53:84	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
29355454	4	38	theme	single	905:910	arg1	substrate					912:920	a single substrate	903:920	a single substrate	903:920	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	6	39	theme	sucrose	1090:1096	arg1	use					1083:1085	The use	1079:1085	The use of sucrose and inulin as co-substrates in the reaction	1079:1140	The use of sucrose and inulin as co-substrates in the reaction simultaneously produced FOS and IOS from sucrose and inulin.
29355454	1	40	theme	Prebiotic	154:162	arg1	substances					164:173	Prebiotic substances	154:173	Prebiotic substances	154:173	Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates.
29355454	8	41	theme	Jerusalem	1327:1335	arg1	artichoke					1337:1345	Jerusalem artichoke	1327:1345	Jerusalem artichoke	1327:1345	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	5	42	theme	inulinase	1033:1041	arg1	%					1003:1003	59.87%	998:1003	59.87% of sucrose	998:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	42	theme	inulinase	1033:1041	arg1	U/g					1026:1028	5.68 U/g	1021:1028	5.68 U/g of inulinase	1021:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	42	theme	inulinase	1033:1041	arg1	inulinase					1033:1041	inulinase	1033:1041	inulinase	1033:1041	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	42	theme	inulinase	1033:1041	arg1	%					985:985	5.95%	981:985	5.95% of inulin	981:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	42	theme	inulinase	1033:1041	arg1	sucrose					1008:1014	sucrose	1008:1014	sucrose	1008:1014	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	42	theme	inulinase	1033:1041	arg1	inulin					990:995	inulin	990:995	inulin	990:995	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	4	43	theme	oligosaccharide	790:804	arg1	production					806:815	oligosaccharide production	790:815	oligosaccharide production through inulinase hydrolysis and synthesis	790:858	Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate.
29355454	5	44	theme	674.82 mg/g	939:949	arg1	yields					931:936	Maximum yields	923:936	Maximum yields (674.82 mg/g substrate)	923:960	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	5	44	theme	674.82 mg/g	939:949	arg1	substrate					951:959	674.82 mg/g substrate	939:959	674.82 mg/g substrate	939:959	Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr.
29355454	3	45	theme	variable	671:678	arg1	Inulin					544:549	Inulin	544:549	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g)	544:656	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	45	theme	variable	671:678	arg1	parameters					680:689	variable parameters	671:689	variable parameters for optimization	671:706	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	46	theme	Aspergillus	630:640	arg1	niger					642:646	Aspergillus niger	630:646	Aspergillus niger	630:646	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	47	theme	%	581:581	arg1	artichoke					566:574	Jerusalem artichoke	556:574	Jerusalem artichoke (5-15% w/v)	556:586	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	3	47	theme	%	581:581	arg1	w/v					583:585	5-15% w/v	577:585	5-15% w/v	577:585	Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for optimization.
29355454	2	48	theme	oligosaccharide	516:530	arg1	production					532:541	oligosaccharide production	516:541	oligosaccharide production	516:541	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	6	49	theme	inulin	1102:1107	arg1	use					1083:1085	The use	1079:1085	The use of sucrose and inulin as co-substrates in the reaction	1079:1140	The use of sucrose and inulin as co-substrates in the reaction simultaneously produced FOS and IOS from sucrose and inulin.
29355454	2	50	theme	fructo-oligosaccharide	285:306	arg1	production					271:280	The production	267:280	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS)	267:344	The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production.
29355454	8	51	theme	high	1273:1276	arg1	substrate					1377:1385	a substrate	1375:1385	a substrate in prebiotics and/or bioethanol production	1375:1428	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	8	51	theme	high	1273:1276	arg1	production					1290:1299	the high value-added production	1269:1299	the high value-added production	1269:1299	Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.
29355454	0	52	from	artichoke	143:151	arg1	co-substrates					92:104	co-substrates	92:104	co-substrates of sucrose and inulin from Jerusalem artichoke	92:151	Optimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichoke.
30806267	6	0	theme	scavenging	1063:1072	arg1	activities					1074:1083	scavenging activities	1063:1083	scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells	1063:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	2	1	theme	column	352:357	arg1	chromatography					359:372	Sephadex G-25 column chromatography	338:372	Sephadex G-25 column chromatography	338:372	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	4	2	theme	sugar-containing	601:616	arg1	components					618:627	The sugar-containing components	597:627	The sugar-containing components in HFC	597:634	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	4	2	theme	sugar-containing	601:616	arg1	oligosaccharides					656:671	mainly neutral oligosaccharides	641:671	mainly neutral oligosaccharides	641:671	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	5	3	theme	crude	948:952	arg1	oligosaccharides					954:969	crude oligosaccharides	948:969	crude oligosaccharides	948:969	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	2	4	theme	G-25	347:350	arg1	chromatography					359:372	Sephadex G-25 column chromatography	338:372	Sephadex G-25 column chromatography	338:372	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	6	5	theme	enzymes	1153:1159	arg1	activity					1129:1136	the activity	1125:1136	the activity of antioxidant enzymes in cells	1125:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	1	6	used	used	175:178	arg2	concentrate					127:137	Fermentation concentrate	114:137	Fermentation concentrate of Hericium caput-medusae (HFC)	114:169	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	6	7	theme	free	1096:1099	arg1	radicals					1101:1108	the free radicals	1092:1108	the free radicals and increasing the activity of antioxidant enzymes in cells	1092:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	6	8	theme	in	1044:1045	arg1	studies					1052:1058	both in vitro and in vivo studies	1026:1058	both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells	1026:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	4	9	from	components	618:627	arg1	HFC					632:634	HFC	632:634	HFC	632:634	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	3	10	theme	infrared	560:567	arg1	spectroscopy					569:580	Fourier-transform infrared spectroscopy	542:580	Fourier-transform infrared spectroscopy	542:580	The two components were partially characterized, and analysis of monosaccharide composition through Fourier-transform infrared spectroscopy was performed.
30806267	4	11	theme	α-pyranose	755:764	arg1	configuration					738:750	the configuration	734:750	the configuration of α-pyranose	734:764	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	5	12	theme	HFCO2	887:891	arg1	activities					873:882	antioxidant activities	861:882	antioxidant activities of HFCO2	861:891	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	3	13	theme	composition	522:532	arg1	analysis					495:502	analysis	495:502	analysis of monosaccharide composition through Fourier-transform infrared spectroscopy	495:580	The two components were partially characterized, and analysis of monosaccharide composition through Fourier-transform infrared spectroscopy was performed.
30806267	5	14	theme	cellular	786:793	arg1	activities					807:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	6	15	dep	radicals	1101:1108	arg1	increasing					1114:1123	increasing	1114:1123	increasing the activity of antioxidant enzymes in cells	1114:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	6	16	theme	antioxidant	1141:1151	arg1	enzymes					1153:1159	antioxidant enzymes	1141:1159	antioxidant enzymes	1141:1159	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	4	17	theme	monosaccharide	682:695	arg1	composition					697:707	the monosaccharide composition	678:707	the monosaccharide composition	678:707	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	4	17	theme	monosaccharide	682:695	arg1	glucose					720:726	glucose	720:726	glucose	720:726	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	4	18	with	glucose	720:726	arg1	configuration					738:750	the configuration	734:750	the configuration of α-pyranose	734:764	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	0	19	theme	Partial	0:6	arg1	Purification					8:19	Partial Purification	0:19	Partial Purification	0:19	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	6	20	dep	in	1031:1032	arg1	vitro					1034:1038	vitro	1034:1038	vitro	1034:1038	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	5	21	theme	neutral	997:1003	arg1	oligosaccharides					1005:1020	neutral oligosaccharides	997:1020	neutral oligosaccharides	997:1020	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	5	22	theme	antioxidant	922:932	arg1	activities					934:943	antioxidant activities	922:943	antioxidant activities of crude oligosaccharides	922:969	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	0	23	theme	Antioxidant	25:35	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	1	24	theme	dialysis	278:285	arg1	methods					287:293	dialysis methods	278:293	dialysis methods	278:293	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	6	25	from	activity	1129:1136	arg1	cells					1164:1168	cells	1164:1168	cells	1164:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	1	26	theme	Fermentation	114:125	arg1	concentrate					127:137	Fermentation concentrate	114:137	Fermentation concentrate of Hericium caput-medusae (HFC)	114:169	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	6	27	theme	H2O2-induced	1204:1215	arg1	reduction					1217:1225	H2O2-induced reduction	1204:1225	H2O2-induced reduction in cell viability	1204:1243	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	6	28	theme	cell	1230:1233	arg1	viability					1235:1243	cell viability	1230:1243	cell viability	1230:1243	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	5	29	theme	oligosaccharides	954:969	arg1	activities					934:943	antioxidant activities	922:943	antioxidant activities of crude oligosaccharides	922:969	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	6	30	from	reduction	1217:1225	arg1	viability					1235:1243	cell viability	1230:1243	cell viability	1230:1243	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	1	31	theme	different	202:210	arg1	oligosaccharides					229:244	two different molecular weight oligosaccharides	198:244	two different molecular weight oligosaccharides (HFCO1, HFCO2)	198:259	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	1	32	theme	molecular	212:220	arg1	oligosaccharides					229:244	two different molecular weight oligosaccharides	198:244	two different molecular weight oligosaccharides (HFCO1, HFCO2)	198:259	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	2	33	theme	purified	402:409	arg1	components					411:420	the two purified components	394:420	the two purified components	394:420	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	0	34	from	caput-medusae	82:94	arg1	Purification					8:19	Partial Purification	0:19	Partial Purification	0:19	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	0	34	from	caput-medusae	82:94	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	5	35	theme	antioxidant	861:871	arg1	activities					873:882	antioxidant activities	861:882	antioxidant activities of HFCO2	861:891	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	1	36	theme	weight	222:227	arg1	oligosaccharides					229:244	two different molecular weight oligosaccharides	198:244	two different molecular weight oligosaccharides (HFCO1, HFCO2)	198:259	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	6	37	theme	in	1031:1032	arg1	studies					1052:1058	both in vitro and in vivo studies	1026:1058	both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells	1026:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	2	38	theme	Sephadex	338:345	arg1	chromatography					359:372	Sephadex G-25 column chromatography	338:372	Sephadex G-25 column chromatography	338:372	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	0	39	theme	Oligosaccharides	51:66	arg1	Purification					8:19	Partial Purification	0:19	Partial Purification	0:19	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	0	39	theme	Oligosaccharides	51:66	arg1	Activities					37:46	Antioxidant Activities	25:46	Antioxidant Activities	25:46	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	1	40	theme	Hericium	142:149	arg1	caput-medusae					151:163	Hericium caput-medusae	142:163	Hericium caput-medusae (HFC)	142:169	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	1	40	theme	Hericium	142:149	arg1	HFC					166:168	HFC	166:168	HFC	166:168	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	1	41	theme	caput-medusae	151:163	arg1	concentrate					127:137	Fermentation concentrate	114:137	Fermentation concentrate of Hericium caput-medusae (HFC)	114:169	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	3	42	theme	Fourier-transform	542:558	arg1	spectroscopy					569:580	Fourier-transform infrared spectroscopy	542:580	Fourier-transform infrared spectroscopy	542:580	The two components were partially characterized, and analysis of monosaccharide composition through Fourier-transform infrared spectroscopy was performed.
30806267	2	43	used	used	379:382	arg2	chromatography					359:372	Sephadex G-25 column chromatography	338:372	Sephadex G-25 column chromatography	338:372	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	2	43	used	used	379:382	arg2	Flow					329:332	DEAE Sepharose Fast Flow	309:332	DEAE Sepharose Fast Flow	309:332	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	2	44	theme	Fast	324:327	arg1	Flow					329:332	DEAE Sepharose Fast Flow	309:332	DEAE Sepharose Fast Flow	309:332	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	1	45	dep	oligosaccharides	229:244	arg1	HFCO1					247:251	HFCO1	247:251	HFCO1	247:251	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	1	45	dep	oligosaccharides	229:244	arg1	HFCO2					254:258	HFCO2	254:258	HFCO2	254:258	Fermentation concentrate of Hericium caput-medusae (HFC) was used in this study and two different molecular weight oligosaccharides (HFCO1, HFCO2) were obtained by dialysis methods.
30806267	6	46	theme	activities	1074:1083	arg1	studies					1052:1058	both in vitro and in vivo studies	1026:1058	both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells	1026:1168	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	5	47	theme	antioxidant	795:805	arg1	activities					807:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
30806267	4	48	with	oligosaccharides	656:671	arg1	configuration					738:750	the configuration	734:750	the configuration of α-pyranose	734:764	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	4	49	theme	neutral	648:654	arg1	components					618:627	The sugar-containing components	597:627	The sugar-containing components in HFC	597:634	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	4	49	theme	neutral	648:654	arg1	oligosaccharides					656:671	mainly neutral oligosaccharides	641:671	mainly neutral oligosaccharides	641:671	The sugar-containing components in HFC were mainly neutral oligosaccharides, and the monosaccharide composition was mainly glucose, with the configuration of α-pyranose.
30806267	0	50	theme	Hericium	73:80	arg1	Agaricomycetes					97:110	Agaricomycetes	97:110	Agaricomycetes	97:110	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	0	50	theme	Hericium	73:80	arg1	caput-medusae					82:94	Hericium caput-medusae	73:94	Hericium caput-medusae (Agaricomycetes)	73:111	Partial Purification and Antioxidant Activities of Oligosaccharides from Hericium caput-medusae (Agaricomycetes).
30806267	6	51	dep	in	1044:1045	arg1	vivo					1047:1050	vivo	1047:1050	vivo	1047:1050	By both in vitro and in vivo studies of scavenging activities toward the free radicals and increasing the activity of antioxidant enzymes in cells, HFCO2 and HFCO2-1 could suppress H2O2-induced reduction in cell viability.
30806267	2	52	dep	obtain	387:392	arg1	HFCO2-1					432:438	HFCO2-1	432:438	HFCO2-1	432:438	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	2	52	dep	obtain	387:392	arg1	HFCO1-1					423:429	HFCO1-1	423:429	HFCO1-1	423:429	Furthermore, DEAE Sepharose Fast Flow and Sephadex G-25 column chromatography were used to obtain the two purified components (HFCO1-1, HFCO2-1).
30806267	3	53	theme	monosaccharide	507:520	arg1	composition					522:532	monosaccharide composition	507:532	monosaccharide composition	507:532	The two components were partially characterized, and analysis of monosaccharide composition through Fourier-transform infrared spectroscopy was performed.
30806267	5	54	theme	chemical	773:780	arg1	activities					807:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities	767:816	Their chemical and cellular antioxidant activities were compared, and the results showed that antioxidant activities of HFCO2 were higher than HFCO2-1 and antioxidant activities of crude oligosaccharides were higher than those of neutral oligosaccharides.
29680605	0	0	theme	sweet	85:89	arg1	films					111:115	sweet potato starch-based films	85:115	sweet potato starch-based films	85:115	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	1	1	theme	composite	338:346	arg1	diffractograms					284:297	diffractograms	284:297	diffractograms	284:297	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	1	theme	composite	338:346	arg1	thermograms					268:278	thermograms	268:278	thermograms	268:278	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	9	2	from	break	1467:1471	arg1	property					1526:1533	better moisture barrier property	1502:1533	better moisture barrier property	1502:1533	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	9	2	from	break	1467:1471	arg1	strength					1489:1496	higher tensile strength	1474:1496	higher tensile strength	1474:1496	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	9	2	from	break	1467:1471	arg1	transmission					1433:1444	higher light transmission	1420:1444	higher light transmission	1420:1444	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	9	2	from	break	1467:1471	arg1	elongation					1453:1462	lower elongation	1447:1462	lower elongation at break	1447:1471	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	3	theme	treatment	158:166	arg1	effect					137:142	the effect	133:142	the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index	133:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	3	4	theme	untreated	707:715	arg1	sample					717:722	the untreated sample	703:722	the untreated sample	703:722	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	3	5	theme	starches	669:676	arg1	lower					683:687	lower	683:687	lower	683:687	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	3	5	theme	starches	669:676	arg1	enthalpies					628:637	the gelatinization enthalpies	609:637	the gelatinization enthalpies of the ultrasonically treated starches	609:676	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	9	6	theme	higher	1474:1479	arg1	strength					1489:1496	higher tensile strength	1474:1496	higher tensile strength	1474:1496	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	7	theme	complexing	250:259	arg1	films					239:243	sweet potato starch-based films	213:243	sweet potato starch-based films	213:243	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	7	theme	complexing	250:259	arg1	index					261:265	the complexing index	246:265	the complexing index	246:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	0	8	theme	starch-based	98:109	arg1	films					111:115	sweet potato starch-based films	85:115	sweet potato starch-based films	85:115	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	0	9	from	Effects	0:6	arg1	properties					71:80	properties	71:80	properties	71:80	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	0	9	from	Effects	0:6	arg1	formation					57:65	amylose-lipid complex formation	35:65	amylose-lipid complex formation	35:65	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	8	10	theme	microscopy	1353:1362	arg1	analysis					1364:1371	atomic force microscopy analysis	1340:1371	atomic force microscopy analysis	1340:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	7	11	theme	films	1213:1217	arg1	surface					1188:1194	the surface	1184:1194	the surface of the composite films	1184:1217	The scanning electronic microscope showed that the surface of the composite films became smooth after being treated by ultrasonication.
29680605	9	12	theme	lower	1447:1451	arg1	elongation					1453:1462	lower elongation	1447:1462	lower elongation at break	1447:1471	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	0	13	theme	potato	91:96	arg1	films					111:115	sweet potato starch-based films	85:115	sweet potato starch-based films	85:115	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	2	14	theme	complex	584:590	arg1	formation					558:566	the formation	554:566	the formation of an inclusion complex	554:590	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	8	15	from	reduction	1298:1306	arg1	roughness					1324:1332	film surface roughness	1311:1332	film surface roughness under atomic force microscopy analysis	1311:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	4	16	theme	40	770:771	arg1	%					772:772	%	772:772	%	772:772	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	2	17	theme	power	511:515	arg1	beneficial					540:549	beneficial	540:549	beneficial	540:549	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	2	17	theme	power	511:515	arg1	ultrasound					525:534	the low power density ultrasound	503:534	the low power density ultrasound	503:534	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	9	18	theme	moisture	1509:1516	arg1	property					1526:1533	better moisture barrier property	1502:1533	better moisture barrier property	1502:1533	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	6	19	theme	relative	1072:1079	arg1	crystallinity					1081:1093	relative crystallinity	1072:1093	relative crystallinity	1072:1093	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	1	20	theme	films	455:459	arg1	properties					437:446	mechanical and moisture barrier properties	405:446	properties	437:446	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	20	theme	films	455:459	arg1	microstructure					385:398	microstructure	385:398	microstructure	385:398	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	20	theme	films	455:459	arg1	transmission					371:382	light transmission	365:382	light transmission	365:382	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	4	21	theme	70	777:778	arg1	%					772:772	%	772:772	%	772:772	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	8	22	theme	atomic	1340:1345	arg1	microscopy					1353:1362	atomic force microscopy	1340:1362	atomic force microscopy analysis	1340:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	0	23	theme	films	111:115	arg1	properties					71:80	properties	71:80	properties	71:80	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	0	23	theme	films	111:115	arg1	formation					57:65	amylose-lipid complex formation	35:65	amylose-lipid complex formation	35:65	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	1	24	theme	light	365:369	arg1	transmission					371:382	light transmission	365:382	light transmission	365:382	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	7	25	theme	scanning	1141:1148	arg1	microscope					1161:1170	The scanning electronic microscope	1137:1170	The scanning electronic microscope	1137:1170	The scanning electronic microscope showed that the surface of the composite films became smooth after being treated by ultrasonication.
29680605	6	26	theme	ultrasonic	1006:1015	arg1	amplitude					1017:1025	the ultrasonic amplitude	1002:1025	the ultrasonic amplitude	1002:1025	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	6	27	theme	diffraction	1046:1056	arg1	intensity					1058:1066	diffraction intensity	1046:1066	diffraction intensity	1046:1066	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	9	28	theme	ultrasonic	1554:1563	arg1	treatment					1565:1573	ultrasonic treatment	1554:1573	ultrasonic treatment	1554:1573	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	4	29	theme	complex	825:831	arg1	ΔH					804:805	ΔH	804:805	ΔH	804:805	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	4	29	theme	complex	825:831	arg1	enthalpy					794:801	the melting enthalpy	782:801	the melting enthalpy (ΔH) of the inclusion complex	782:831	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	1	30	from	effect	137:142	arg1	properties					175:184	the properties	171:184	the properties of sweet potato starch and sweet potato starch-based films, the complexing index	171:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	5	31	theme	untreated	919:927	arg1	samples					929:935	the untreated samples	915:935	the untreated samples	915:935	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	4	32	theme	inclusion	815:823	arg1	complex					825:831	the inclusion complex	811:831	the inclusion complex	811:831	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	4	33	theme	ultrasonic	734:743	arg1	amplitude					745:753	the ultrasonic amplitude	730:753	the ultrasonic amplitude	730:753	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	1	34	theme	sweet	189:193	arg1	starch					202:207	sweet potato starch	189:207	sweet potato starch	189:207	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	0	35	theme	treatment	22:30	arg1	Effects					0:6	Effects	0:6	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.	0:116	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	8	36	theme	film	1311:1314	arg1	roughness					1324:1332	film surface roughness	1311:1332	film surface roughness under atomic force microscopy analysis	1311:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	9	37	with	films	1378:1382	arg1	treatment					1400:1408	ultrasonic treatment	1389:1408	ultrasonic treatment	1389:1408	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	6	38	dep	intensity	1058:1066	arg1	the					1042:1044	the	1042:1044	the	1042:1044	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	1	39	theme	potato	195:200	arg1	starch					202:207	sweet potato starch	189:207	sweet potato starch	189:207	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	0	40	theme	ultrasonic	11:20	arg1	treatment					22:30	ultrasonic treatment	11:30	ultrasonic treatment	11:30	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	5	41	theme	samples	929:935	arg1	intensity					902:910	the diffraction intensity	886:910	the diffraction intensity of the untreated samples	886:935	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	5	41	theme	samples	929:935	arg1	weaker					941:946	weaker	941:946	weaker	941:946	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	1	42	theme	starch	202:207	arg1	properties					175:184	the properties	171:184	the properties of sweet potato starch and sweet potato starch-based films, the complexing index	171:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	0	43	theme	amylose-lipid	35:47	arg1	formation					57:65	amylose-lipid complex formation	35:65	amylose-lipid complex formation	35:65	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	3	44	theme	treated	661:667	arg1	starches					669:676	the ultrasonically treated starches	642:676	the ultrasonically treated starches	642:676	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	4	45	dep	%	772:772	arg1	to					774:775	to	774:775	to	774:775	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	5	46	theme	X-ray	854:858	arg1	diffraction					860:870	X-ray diffraction	854:870	X-ray diffraction	854:870	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	7	47	theme	electronic	1150:1159	arg1	microscope					1161:1170	The scanning electronic microscope	1137:1170	The scanning electronic microscope	1137:1170	The scanning electronic microscope showed that the surface of the composite films became smooth after being treated by ultrasonication.
29680605	9	48	theme	ultrasonic	1389:1398	arg1	treatment					1400:1408	ultrasonic treatment	1389:1408	ultrasonic treatment	1389:1408	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	8	49	theme	surface	1316:1322	arg1	roughness					1324:1332	film surface roughness	1311:1332	film surface roughness under atomic force microscopy analysis	1311:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	9	50	theme	higher	1420:1425	arg1	transmission					1433:1444	higher light transmission	1420:1444	higher light transmission	1420:1444	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	51	theme	sweet	213:217	arg1	films					239:243	sweet potato starch-based films	213:243	sweet potato starch-based films	213:243	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	51	theme	sweet	213:217	arg1	index					261:265	the complexing index	246:265	the complexing index	246:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	52	theme	sweet	306:310	arg1	composite					338:346	the sweet potato starch-lauric acid composite	302:346	the sweet potato starch-lauric acid composite	302:346	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	2	53	theme	inclusion	574:582	arg1	complex					584:590	an inclusion complex	571:590	an inclusion complex	571:590	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	5	54	theme	diffraction	890:900	arg1	intensity					902:910	the diffraction intensity	886:910	the diffraction intensity of the untreated samples	886:935	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	5	54	theme	diffraction	890:900	arg1	weaker					941:946	weaker	941:946	weaker	941:946	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	0	55	theme	complex	49:55	arg1	formation					57:65	amylose-lipid complex formation	35:65	amylose-lipid complex formation	35:65	Effects of ultrasonic treatment on amylose-lipid complex formation and properties of sweet potato starch-based films.
29680605	1	56	theme	potato	312:317	arg1	composite					338:346	the sweet potato starch-lauric acid composite	302:346	the sweet potato starch-lauric acid composite	302:346	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	57	theme	mechanical	405:414	arg1	properties					437:446	mechanical and moisture barrier properties	405:446	properties	437:446	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	7	58	theme	composite	1203:1211	arg1	films					1213:1217	the composite films	1199:1217	the composite films	1199:1217	The scanning electronic microscope showed that the surface of the composite films became smooth after being treated by ultrasonication.
29680605	9	59	theme	barrier	1518:1524	arg1	property					1526:1533	better moisture barrier property	1502:1533	better moisture barrier property	1502:1533	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	60	theme	starch-lauric	319:331	arg1	composite					338:346	the sweet potato starch-lauric acid composite	302:346	the sweet potato starch-lauric acid composite	302:346	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	3	61	theme	gelatinization	613:626	arg1	lower					683:687	lower	683:687	lower	683:687	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	3	61	theme	gelatinization	613:626	arg1	enthalpies					628:637	the gelatinization enthalpies	609:637	the gelatinization enthalpies of the ultrasonically treated starches	609:676	In thermograms, the gelatinization enthalpies of the ultrasonically treated starches were lower than those of the untreated sample.
29680605	6	62	theme	complex	1108:1114	arg1	crystallinity					1081:1093	relative crystallinity	1072:1093	relative crystallinity	1072:1093	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	6	62	theme	complex	1108:1114	arg1	intensity					1058:1066	diffraction intensity	1046:1066	diffraction intensity	1046:1066	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	2	63	theme	low	507:509	arg1	beneficial					540:549	beneficial	540:549	beneficial	540:549	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	2	63	theme	low	507:509	arg1	ultrasound					525:534	the low power density ultrasound	503:534	the low power density ultrasound	503:534	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	1	64	theme	potato	219:224	arg1	films					239:243	sweet potato starch-based films	213:243	sweet potato starch-based films	213:243	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	64	theme	potato	219:224	arg1	index					261:265	the complexing index	246:265	the complexing index	246:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	65	theme	moisture	420:427	arg1	properties					437:446	mechanical and moisture barrier properties	405:446	properties	437:446	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	5	66	theme	treated	980:986	arg1	samples					988:994	the ultrasonically treated samples	961:994	the ultrasonically treated samples	961:994	X-ray diffraction revealed that the diffraction intensity of the untreated samples was weaker than that of the ultrasonically treated samples.
29680605	6	67	theme	inclusion	1098:1106	arg1	complex					1108:1114	inclusion complex	1098:1114	inclusion complex	1098:1114	When the ultrasonic amplitude was above 40%, the diffraction intensity and relative crystallinity of inclusion complex gradually decreased.
29680605	8	68	theme	force	1347:1351	arg1	microscopy					1353:1362	atomic force microscopy	1340:1362	atomic force microscopy analysis	1340:1371	Ultrasonication led to a reduction in film surface roughness under atomic force microscopy analysis.
29680605	1	69	theme	starch-based	226:237	arg1	films					239:243	sweet potato starch-based films	213:243	sweet potato starch-based films	213:243	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	69	theme	starch-based	226:237	arg1	index					261:265	the complexing index	246:265	the complexing index	246:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	9	70	theme	better	1502:1507	arg1	property					1526:1533	better moisture barrier property	1502:1533	better moisture barrier property	1502:1533	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	71	theme	barrier	429:435	arg1	properties					437:446	mechanical and moisture barrier properties	405:446	properties	437:446	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	4	72	theme	melting	786:792	arg1	ΔH					804:805	ΔH	804:805	ΔH	804:805	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	4	72	theme	melting	786:792	arg1	enthalpy					794:801	the melting enthalpy	782:801	the melting enthalpy (ΔH) of the inclusion complex	782:831	With the ultrasonic amplitude increased from 40% to 70%, the melting enthalpy (ΔH) of the inclusion complex gradually decreased.
29680605	9	73	theme	tensile	1481:1487	arg1	strength					1489:1496	higher tensile strength	1474:1496	higher tensile strength	1474:1496	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	74	theme	films	239:243	arg1	properties					175:184	the properties	171:184	the properties of sweet potato starch and sweet potato starch-based films, the complexing index	171:265	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	1	75	theme	acid	333:336	arg1	composite					338:346	the sweet potato starch-lauric acid composite	302:346	the sweet potato starch-lauric acid composite	302:346	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	9	76	theme	light	1427:1431	arg1	transmission					1433:1444	higher light transmission	1420:1444	higher light transmission	1420:1444	The films with ultrasonic treatment exhibited higher light transmission, lower elongation at break, higher tensile strength and better moisture barrier property than those without ultrasonic treatment.
29680605	1	77	theme	ultrasonic	147:156	arg1	treatment					158:166	ultrasonic treatment	147:166	ultrasonic treatment	147:166	To investigate the effect of ultrasonic treatment on the properties of sweet potato starch and sweet potato starch-based films, the complexing index, thermograms and diffractograms of the sweet potato starch-lauric acid composite were tested, and light transmission, microstructure, and mechanical and moisture barrier properties of the films were measured.
29680605	2	78	theme	density	517:523	arg1	beneficial					540:549	beneficial	540:549	beneficial	540:549	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
29680605	2	78	theme	density	517:523	arg1	ultrasound					525:534	the low power density ultrasound	503:534	the low power density ultrasound	503:534	The results indicated that the low power density ultrasound was beneficial to the formation of an inclusion complex.
30590468	0	0	theme	Underlying	68:77	arg1	Mechanism					79:87	Underlying Mechanism	68:87	Underlying Mechanism	68:87	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	8	1	theme	taste	1173:1177	arg1	lactisole					1189:1197	a sweet taste inhibitor lactisole	1165:1197	a sweet taste inhibitor lactisole	1165:1197	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	10	2	theme	acarbose	1367:1374	arg1	detection					1354:1362	the relative sweetness detection	1331:1362	the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose)	1331:1478	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	3	3	theme	saccharide	382:391	arg1	analogs					393:399	saccharide analogs	382:399	saccharide analogs	382:399	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	1	4	contain	possess	125:131	arg1	saccharides					113:123	sweet-tasting saccharides	99:123	sweet-tasting saccharides	99:123	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	1	4	contain	possess	125:131	arg2	structures					151:160	similar molecular structures	133:160	similar molecular structures	133:160	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	12	5	theme	saccharides	1860:1870	arg1	sweetness					1847:1855	the relative sweetness	1834:1855	the relative sweetness of saccharides	1834:1870	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	1	6	theme	relative	169:176	arg1	sweetness					178:186	their relative sweetness	163:186	their relative sweetness	163:186	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	3	7	theme	relative	420:427	arg1	sweetness					429:437	their relative sweetness	414:437	their relative sweetness	414:437	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	12	8	theme	specific	1731:1738	arg1	features					1750:1757	specific molecular features	1731:1757	specific molecular features (e.g., degree of polymerization and monomer composition)	1731:1814	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	8	9	theme	sweet	1052:1056	arg1	taste					1058:1062	the sweet taste	1048:1062	the sweet taste of acarbose and maltotriose	1048:1090	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	10	10	theme	sweet-tasting	1400:1412	arg1	mono-					1414:1418	other sweet-tasting mono-	1394:1418	other sweet-tasting mono-	1394:1418	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	12	11	theme	molecular	1740:1748	arg1	features					1750:1757	specific molecular features	1731:1757	specific molecular features (e.g., degree of polymerization and monomer composition)	1731:1814	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	5	12	theme	overall	690:696	arg1	resemblance					698:708	an overall resemblance	687:708	an overall resemblance to a glucose-based oligosaccharide	687:743	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	6	13	theme	other	799:803	arg1	studies					805:811	other studies	799:811	other studies	799:811	During other studies, we recognized that acarbose can also elicit sweet taste.
30590468	8	14	theme	lactisole	1189:1197	arg1	presence					1153:1160	the presence	1149:1160	the presence of a sweet taste inhibitor lactisole	1149:1197	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	10	15	theme	sweetness	1344:1352	arg1	detection					1354:1362	the relative sweetness detection	1331:1362	the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose)	1331:1478	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	9	16	theme	human	1252:1256	arg1	hT1R2/hT1R3					1280:1290	hT1R2/hT1R3	1280:1290	hT1R2/hT1R3	1280:1290	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	9	16	theme	human	1252:1256	arg1	receptor					1270:1277	the human sweet taste receptor	1248:1277	the human sweet taste receptor	1248:1277	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	7	17	theme	acarbose	946:953	arg1	mechanism					933:941	the underlying taste detection mechanism	902:941	the underlying taste detection mechanism of acarbose	902:953	Here, we formally investigated the underlying taste detection mechanism of acarbose, while confirming our previous findings for maltotriose.
30590468	3	18	from	insight	451:457	arg1	mechanisms					466:475	the mechanisms	462:475	the mechanisms underlying sweetness potency	462:504	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	12	19	theme	composition	1803:1813	arg1	degree					1766:1771	degree	1766:1771	degree of polymerization and monomer composition	1766:1813	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	8	20	theme	maltotriose	1080:1090	arg1	taste					1058:1062	the sweet taste	1048:1062	the sweet taste of acarbose and maltotriose	1048:1090	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	7	21	theme	detection	923:931	arg1	mechanism					933:941	the underlying taste detection mechanism	902:941	the underlying taste detection mechanism of acarbose	902:953	Here, we formally investigated the underlying taste detection mechanism of acarbose, while confirming our previous findings for maltotriose.
30590468	0	22	theme	Sweet	4:8	arg1	Taste					10:14	The Sweet Taste	0:14	The Sweet Taste of Acarbose and Maltotriose	0:42	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	5	23	theme	glucose-based	715:727	arg1	oligosaccharide					729:743	a glucose-based oligosaccharide	713:743	a glucose-based oligosaccharide	713:743	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	9	24	theme	taste	1264:1268	arg1	hT1R2/hT1R3					1280:1290	hT1R2/hT1R3	1280:1290	hT1R2/hT1R3	1280:1290	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	9	24	theme	taste	1264:1268	arg1	receptor					1270:1277	the human sweet taste receptor	1248:1277	the human sweet taste receptor	1248:1277	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	4	25	theme	sweet	604:608	arg1	taste					610:614	sweet taste	604:614	sweet taste	604:614	Maltotriose is a short-chain glucose-based oligosaccharide, which we recently reported to elicit sweet taste.
30590468	10	26	dep	mono-	1414:1418	arg1	maltose					1458:1464	maltose	1458:1464	maltose	1458:1464	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	10	26	dep	mono-	1414:1418	arg1	sucrose					1471:1477	sucrose	1471:1477	sucrose	1471:1477	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	10	26	dep	mono-	1414:1418	arg1	fructose					1448:1455	fructose	1448:1455	fructose	1448:1455	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	10	26	dep	mono-	1414:1418	arg1	glucose					1439:1445	glucose	1439:1445	glucose	1439:1445	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	6	27	theme	sweet	858:862	arg1	taste					864:868	sweet taste	858:868	sweet taste	858:868	During other studies, we recognized that acarbose can also elicit sweet taste.
30590468	7	28	theme	underlying	906:915	arg1	mechanism					933:941	the underlying taste detection mechanism	902:941	the underlying taste detection mechanism of acarbose	902:953	Here, we formally investigated the underlying taste detection mechanism of acarbose, while confirming our previous findings for maltotriose.
30590468	5	29	contain	has	683:685	arg1	pseudo-saccharide					660:676	a pseudo-saccharide	658:676	a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog	658:789	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	5	29	contain	has	683:685	arg2	resemblance					698:708	an overall resemblance	687:708	an overall resemblance to a glucose-based oligosaccharide	687:743	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	5	29	contain	has	683:685	arg1	Acarbose					617:624	Acarbose	617:624	Acarbose	617:624	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	5	29	contain	has	683:685	arg1	analog					784:789	a structural analog	771:789	a structural analog	771:789	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	8	30	theme	aqueous	1095:1101	arg1	solutions					1103:1111	aqueous solutions	1095:1111	aqueous solutions	1095:1111	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	11	31	theme	acarbose	1602:1609	arg1	discriminability					1582:1597	the discriminability	1578:1597	the discriminability of acarbose	1578:1609	Whereas maltotriose was found to have a similar discriminability profile to glucose and maltose, the discriminability of acarbose matched that of fructose at the concentrations tested (18, 32, and 56 mM).
30590468	0	32	dep	Detection	54:62	arg1	Taste					10:14	The Sweet Taste	0:14	The Sweet Taste of Acarbose and Maltotriose	0:42	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	7	33	theme	taste	917:921	arg1	mechanism					933:941	the underlying taste detection mechanism	902:941	the underlying taste detection mechanism of acarbose	902:953	Here, we formally investigated the underlying taste detection mechanism of acarbose, while confirming our previous findings for maltotriose.
30590468	9	34	theme	receptor	1270:1277	arg1	ligands					1237:1243	ligands	1237:1243	ligands of the human sweet taste receptor, hT1R2/hT1R3	1237:1290	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	9	34	theme	receptor	1270:1277	arg1	both					1228:1231	both	1228:1231	both	1228:1231	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	12	35	theme	monomer	1795:1801	arg1	composition					1803:1813	monomer composition	1795:1813	monomer composition	1795:1813	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	3	36	theme	certain	332:338	arg1	features					351:358	certain structural features	332:358	certain structural features of saccharides and/or saccharide analogs	332:399	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	4	37	theme	short-chain	524:534	arg1	Maltotriose					507:517	Maltotriose	507:517	Maltotriose	507:517	Maltotriose is a short-chain glucose-based oligosaccharide, which we recently reported to elicit sweet taste.
30590468	4	37	theme	short-chain	524:534	arg1	oligosaccharide					550:564	a short-chain glucose-based oligosaccharide	522:564	a short-chain glucose-based oligosaccharide	522:564	Maltotriose is a short-chain glucose-based oligosaccharide, which we recently reported to elicit sweet taste.
30590468	1	38	theme	sweet-tasting	99:111	arg1	saccharides					113:123	sweet-tasting saccharides	99:123	sweet-tasting saccharides	99:123	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	1	39	theme	considerable	206:217	arg1	degree					219:224	a considerable degree	204:224	a considerable degree	204:224	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	10	40	theme	relative	1335:1342	arg1	detection					1354:1362	the relative sweetness detection	1331:1362	the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose)	1331:1478	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	2	41	theme	saccharide	252:261	arg1	interrelationships					283:300	saccharide structure/sweetness interrelationships	252:300	saccharide structure/sweetness interrelationships	252:300	Current understanding of saccharide structure/sweetness interrelationships is limited.
30590468	0	42	theme	Acarbose	19:26	arg1	Taste					10:14	The Sweet Taste	0:14	The Sweet Taste of Acarbose and Maltotriose	0:42	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	8	43	theme	acarbose	1067:1074	arg1	taste					1058:1062	the sweet taste	1048:1062	the sweet taste of acarbose and maltotriose	1048:1090	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	3	44	theme	sweetness	488:496	arg1	potency					498:504	sweetness potency	488:504	sweetness potency	488:504	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	12	45	dep	degree	1766:1771	arg1	e.g.					1760:1763	e.g.	1760:1763	e.g.	1760:1763	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	10	46	theme	other	1394:1398	arg1	mono-					1414:1418	other sweet-tasting mono-	1394:1418	other sweet-tasting mono-	1394:1418	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	8	47	theme	sweet	1167:1171	arg1	lactisole					1189:1197	a sweet taste inhibitor lactisole	1165:1197	a sweet taste inhibitor lactisole	1165:1197	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	4	48	theme	glucose-based	536:548	arg1	Maltotriose					507:517	Maltotriose	507:517	Maltotriose	507:517	Maltotriose is a short-chain glucose-based oligosaccharide, which we recently reported to elicit sweet taste.
30590468	4	48	theme	glucose-based	536:548	arg1	oligosaccharide					550:564	a short-chain glucose-based oligosaccharide	522:564	a short-chain glucose-based oligosaccharide	522:564	Maltotriose is a short-chain glucose-based oligosaccharide, which we recently reported to elicit sweet taste.
30590468	12	49	theme	relative	1838:1845	arg1	sweetness					1847:1855	the relative sweetness	1834:1855	the relative sweetness of saccharides	1834:1870	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	5	50	theme	α-glucosidase	630:642	arg1	Acarbose					617:624	Acarbose	617:624	Acarbose	617:624	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	5	50	theme	α-glucosidase	630:642	arg1	inhibitor					644:652	an α-glucosidase inhibitor	627:652	an α-glucosidase inhibitor	627:652	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	0	51	theme	Maltotriose	32:42	arg1	Taste					10:14	The Sweet Taste	0:14	The Sweet Taste of Acarbose and Maltotriose	0:42	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	3	52	theme	saccharides	363:373	arg1	features					351:358	certain structural features	332:358	certain structural features of saccharides and/or saccharide analogs	332:399	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	2	53	theme	Current	227:233	arg1	understanding					235:247	Current understanding	227:247	Current understanding of saccharide structure/sweetness interrelationships	227:300	Current understanding of saccharide structure/sweetness interrelationships is limited.
30590468	11	54	theme	similar	1521:1527	arg1	profile					1546:1552	a similar discriminability profile	1519:1552	a similar discriminability profile to glucose and maltose	1519:1575	Whereas maltotriose was found to have a similar discriminability profile to glucose and maltose, the discriminability of acarbose matched that of fructose at the concentrations tested (18, 32, and 56 mM).
30590468	3	55	theme	structural	340:349	arg1	features					351:358	certain structural features	332:358	certain structural features of saccharides and/or saccharide analogs	332:399	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	9	56	theme	sweet	1258:1262	arg1	hT1R2/hT1R3					1280:1290	hT1R2/hT1R3	1280:1290	hT1R2/hT1R3	1280:1290	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	9	56	theme	sweet	1258:1262	arg1	receptor					1270:1277	the human sweet taste receptor	1248:1277	the human sweet taste receptor	1248:1277	These findings support that both are ligands of the human sweet taste receptor, hT1R2/hT1R3.
30590468	10	57	theme	maltotriose	1377:1387	arg1	detection					1354:1362	the relative sweetness detection	1331:1362	the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose)	1331:1478	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	1	58	theme	similar	133:139	arg1	structures					151:160	similar molecular structures	133:160	similar molecular structures	133:160	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	11	59	theme	discriminability	1529:1544	arg1	profile					1546:1552	a similar discriminability profile	1519:1552	a similar discriminability profile to glucose and maltose	1519:1575	Whereas maltotriose was found to have a similar discriminability profile to glucose and maltose, the discriminability of acarbose matched that of fructose at the concentrations tested (18, 32, and 56 mM).
30590468	5	60	theme	structural	773:782	arg1	pseudo-saccharide					660:676	a pseudo-saccharide	658:676	a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog	658:789	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	5	60	theme	structural	773:782	arg1	analog					784:789	a structural analog	771:789	a structural analog	771:789	Acarbose, an α-glucosidase inhibitor, is a pseudo-saccharide that has an overall resemblance to a glucose-based oligosaccharide and thus may be viewed as a structural analog.
30590468	8	61	theme	inhibitor	1179:1187	arg1	lactisole					1189:1197	a sweet taste inhibitor lactisole	1165:1197	a sweet taste inhibitor lactisole	1165:1197	We found that subjects could detect the sweet taste of acarbose and maltotriose in aqueous solutions but were not able to detect them in the presence of a sweet taste inhibitor lactisole.
30590468	1	62	theme	molecular	141:149	arg1	structures					151:160	similar molecular structures	133:160	similar molecular structures	133:160	Although sweet-tasting saccharides possess similar molecular structures, their relative sweetness often varies to a considerable degree.
30590468	7	63	theme	previous	977:984	arg1	findings					986:993	our previous findings	973:993	our previous findings for maltotriose	973:1009	Here, we formally investigated the underlying taste detection mechanism of acarbose, while confirming our previous findings for maltotriose.
30590468	3	64	theme	analogs	393:399	arg1	features					351:358	certain structural features	332:358	certain structural features of saccharides and/or saccharide analogs	332:399	Understanding how certain structural features of saccharides and/or saccharide analogs correlate to their relative sweetness can provide insight on the mechanisms underlying sweetness potency.
30590468	10	65	theme	separate	1298:1305	arg1	experiment					1307:1316	a separate experiment	1296:1316	a separate experiment	1296:1316	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30590468	2	66	theme	interrelationships	283:300	arg1	understanding					235:247	Current understanding	227:247	Current understanding of saccharide structure/sweetness interrelationships	227:300	Current understanding of saccharide structure/sweetness interrelationships is limited.
30590468	12	67	theme	polymerization	1776:1789	arg1	degree					1766:1771	degree	1766:1771	degree of polymerization and monomer composition	1766:1813	These findings are discussed in terms of how specific molecular features (e.g., degree of polymerization and monomer composition) may contribute to the relative sweetness of saccharides.
30590468	11	68	contain	have	1514:1517	arg2	profile					1546:1552	a similar discriminability profile	1519:1552	a similar discriminability profile to glucose and maltose	1519:1575	Whereas maltotriose was found to have a similar discriminability profile to glucose and maltose, the discriminability of acarbose matched that of fructose at the concentrations tested (18, 32, and 56 mM).
30590468	11	68	contain	have	1514:1517	arg1	maltotriose					1489:1499	maltotriose	1489:1499	maltotriose	1489:1499	Whereas maltotriose was found to have a similar discriminability profile to glucose and maltose, the discriminability of acarbose matched that of fructose at the concentrations tested (18, 32, and 56 mM).
30590468	0	69	theme	Relative	45:52	arg1	Detection					54:62	Relative Detection	45:62	Relative Detection	45:62	The Sweet Taste of Acarbose and Maltotriose: Relative Detection and Underlying Mechanism.
30590468	2	70	theme	structure/sweetness	263:281	arg1	interrelationships					283:300	saccharide structure/sweetness interrelationships	252:300	saccharide structure/sweetness interrelationships	252:300	Current understanding of saccharide structure/sweetness interrelationships is limited.
30590468	10	71	theme	mono-	1414:1418	arg1	detection					1354:1362	the relative sweetness detection	1331:1362	the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose)	1331:1478	In a separate experiment, we measured the relative sweetness detection of acarbose, maltotriose, and other sweet-tasting mono- and disaccharides (glucose, fructose, maltose, and sucrose).
30017988	1	0	theme	method	308:313	arg1	use					283:285	the use	279:285	the use of the freeze-drying method	279:313	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	9	1	theme	cell	1069:1072	arg1	growth					1074:1079	cell growth	1069:1079	cell growth	1069:1079	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	9	1	theme	cell	1069:1072	arg1	SaOS-2					1111:1116	human osteosarcoma cell line SaOS-2	1082:1116	human osteosarcoma cell line SaOS-2	1082:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	4	2	theme	collagen	547:554	arg1	blends					527:532	the blends	523:532	the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite	523:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	5	3	theme	SEM	635:637	arg1	images					639:644	SEM images	635:644	SEM images	635:644	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	10	4	theme	obtained	1132:1139	arg1	results					1141:1147	The obtained results	1128:1147	The obtained results	1128:1147	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	0	5	with	collagen	71:78	arg1	addition					128:135	nano-hydroxyapatite addition	108:135	nano-hydroxyapatite addition	108:135	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	4	6	with	chitosan	537:544	arg1	hydroxyapatite					585:598	hydroxyapatite	585:598	hydroxyapatite	585:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	10	7	theme	materials	1274:1282	arg1	cells					1233:1237	cells	1233:1237	cells	1233:1237	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	10	7	theme	materials	1274:1282	arg1	parameters					1218:1227	the mechanical parameters	1203:1227	the mechanical parameters	1203:1227	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	5	8	theme	pores	711:715	arg1	size					717:720	the pores size ~250 μm	707:728	the pores size ~250 μm	707:728	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	9	9	theme	human	1082:1086	arg1	growth					1074:1079	cell growth	1069:1079	cell growth	1069:1079	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	9	9	theme	human	1082:1086	arg1	SaOS-2					1111:1116	human osteosarcoma cell line SaOS-2	1082:1116	human osteosarcoma cell line SaOS-2	1082:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	2	10	theme	mechanical	383:392	arg1	analyses					353:360	different analyses	343:360	different analyses	343:360	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	10	theme	mechanical	383:392	arg1	testing					394:400	mechanical testing	383:400	mechanical testing	383:400	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	9	11	theme	osteosarcoma	1088:1099	arg1	growth					1074:1079	cell growth	1069:1079	cell growth	1069:1079	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	9	11	theme	osteosarcoma	1088:1099	arg1	SaOS-2					1111:1116	human osteosarcoma cell line SaOS-2	1082:1116	human osteosarcoma cell line SaOS-2	1082:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	2	12	theme	SEM	368:370	arg1	analyses					353:360	different analyses	343:360	different analyses	343:360	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	12	theme	SEM	368:370	arg1	images					372:377	SEM images	368:377	SEM images	368:377	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	0	13	theme	nano-hydroxyapatite	108:126	arg1	addition					128:135	nano-hydroxyapatite addition	108:135	nano-hydroxyapatite addition	108:135	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	10	14	theme	biological	1239:1248	arg1	response					1250:1257	biological response	1239:1257	biological response	1239:1257	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	9	15	theme	cell	1101:1104	arg1	growth					1074:1079	cell growth	1069:1079	cell growth	1069:1079	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	9	15	theme	cell	1101:1104	arg1	SaOS-2					1111:1116	human osteosarcoma cell line SaOS-2	1082:1116	human osteosarcoma cell line SaOS-2	1082:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	1	16	theme	collagen	173:180	arg1	mixtures					220:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures	157:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp)	157:258	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	6	17	theme	research	755:762	arg1	purpose					740:746	The main purpose	731:746	The main purpose of the research	731:762	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	9	18	theme	line	1106:1109	arg1	growth					1074:1079	cell growth	1069:1079	cell growth	1069:1079	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	9	18	theme	line	1106:1109	arg1	SaOS-2					1111:1116	human osteosarcoma cell line SaOS-2	1082:1116	human osteosarcoma cell line SaOS-2	1082:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	6	19	from	influence	782:790	arg1	properties					864:873	the glycosaminoglycans-enriched scaffolds properties	822:873	the glycosaminoglycans-enriched scaffolds properties	822:873	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	0	20	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of scaffolds	0:38	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	4	21	from	environment	622:632	arg1	stable					604:609	stable	604:609	stable	604:609	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	4	21	from	environment	622:632	arg1	scaffolds					504:512	the scaffolds	500:512	the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite	500:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	10	22	dep	parameters	1218:1227	arg1	response					1250:1257	biological response	1239:1257	biological response	1239:1257	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	0	23	theme	scaffolds	30:38	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties of scaffolds	0:38	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	5	24	theme	porous	675:680	arg1	structure					692:700	a porous scaffolds structure	673:700	a porous scaffolds structure with the pores size ~250 μm	673:728	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	0	25	with	glycosaminoglycans	84:101	arg1	addition					128:135	nano-hydroxyapatite addition	108:135	nano-hydroxyapatite addition	108:135	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	3	26	theme	biocompatibility	435:450	arg1	tests					452:456	swelling behavior and biocompatibility tests	413:456	tests	452:456	Moreover, swelling behavior and biocompatibility tests were carried out.
30017988	4	27	theme	chitosan	537:544	arg1	blends					527:532	the blends	523:532	the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite	523:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	5	28	theme	scaffolds	682:690	arg1	structure					692:700	a porous scaffolds structure	673:700	a porous scaffolds structure with the pores size ~250 μm	673:728	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	1	29	theme	chitosan	157:164	arg1	mixtures					220:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures	157:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp)	157:258	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	5	30	with	structure	692:700	arg1	size					717:720	the pores size ~250 μm	707:728	the pores size ~250 μm	707:728	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	5	31	theme	structure	692:700	arg1	observation					658:668	the observation	654:668	the observation of a porous scaffolds structure with the pores size ~250 μm	654:728	SEM images allowed the observation of a porous scaffolds structure with the pores size ~250 μm.
30017988	6	32	theme	scaffolds	854:862	arg1	properties					864:873	the glycosaminoglycans-enriched scaffolds properties	822:873	the glycosaminoglycans-enriched scaffolds properties	822:873	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	4	33	from	stable	604:609	arg1	environment					622:632	aqueous environment	614:632	aqueous environment	614:632	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	7	34	theme	swelling	910:917	arg1	parameters					934:943	swelling and mechanical parameters	910:943	swelling and mechanical parameters	910:943	The physicochemical properties as swelling and mechanical parameters were tested.
30017988	6	35	theme	glycosaminoglycans-enriched	826:852	arg1	properties					864:873	the glycosaminoglycans-enriched scaffolds properties	822:873	the glycosaminoglycans-enriched scaffolds properties	822:873	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	4	36	theme	aqueous	614:620	arg1	environment					622:632	aqueous environment	614:632	aqueous environment	614:632	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	6	37	theme	main	735:738	arg1	purpose					740:746	The main purpose	731:746	The main purpose of the research	731:762	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	9	38	theme	preliminary	1017:1027	arg1	assessment					1029:1038	the preliminary assessment	1013:1038	the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2	1013:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	4	39	with	glycosaminoglycans	561:578	arg1	hydroxyapatite					585:598	hydroxyapatite	585:598	hydroxyapatite	585:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	10	40	theme	studied	1266:1272	arg1	materials					1274:1282	the studied materials	1262:1282	the studied materials	1262:1282	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	9	41	used	used	1122:1125	arg2	assessment					1029:1038	the preliminary assessment	1013:1038	the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2	1013:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	6	42	theme	addition	810:817	arg1	influence					782:790	the influence	778:790	the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties	778:873	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	7	43	theme	mechanical	923:932	arg1	parameters					934:943	swelling and mechanical parameters	910:943	swelling and mechanical parameters	910:943	The physicochemical properties as swelling and mechanical parameters were tested.
30017988	10	44	theme	mechanical	1207:1216	arg1	parameters					1218:1227	the mechanical parameters	1203:1227	the mechanical parameters	1203:1227	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30017988	6	45	theme	hydroxyapatite	795:808	arg1	addition					810:817	hydroxyapatite addition	795:817	hydroxyapatite addition	795:817	The main purpose of the research was to detect the influence of hydroxyapatite addition on the glycosaminoglycans-enriched scaffolds properties.
30017988	2	46	dep	analyses	353:360	arg1	analyses					353:360	different analyses	343:360	different analyses	343:360	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	46	dep	analyses	353:360	arg1	images					372:377	SEM images	368:377	SEM images	368:377	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	46	dep	analyses	353:360	arg1	testing					394:400	mechanical testing	383:400	mechanical testing	383:400	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	8	47	theme	scaffolds	962:970	arg1	structure					972:980	The scaffolds structure	958:980	The scaffolds structure	958:980	The scaffolds structure was observed by SEM.
30017988	9	48	theme	scaffolds	1043:1051	arg1	suitability					1053:1063	scaffolds suitability	1043:1063	scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2	1043:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	4	49	theme	glycosaminoglycans	561:578	arg1	blends					527:532	the blends	523:532	the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite	523:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	1	50	theme	glycosaminoglycans	194:211	arg1	mixtures					220:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures	157:227	chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp)	157:258	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	4	51	with	collagen	547:554	arg1	hydroxyapatite					585:598	hydroxyapatite	585:598	hydroxyapatite	585:598	The results showed that the scaffolds based on the blends of chitosan, collagen, and glycosaminoglycans with hydroxyapatite are stable in aqueous environment.
30017988	1	52	with	mixtures	220:227	arg1	nano-hydroxyapatite					234:252	nano-hydroxyapatite	234:252	nano-hydroxyapatite (HAp)	234:258	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	1	52	with	mixtures	220:227	arg1	HAp					255:257	HAp	255:257	HAp	255:257	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	2	53	theme	different	343:351	arg1	analyses					353:360	different analyses	343:360	different analyses	343:360	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	53	theme	different	343:351	arg1	images					372:377	SEM images	368:377	SEM images	368:377	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	2	53	theme	different	343:351	arg1	testing					394:400	mechanical testing	383:400	mechanical testing	383:400	They were characterized by different analyses, e.g. SEM images and mechanical testing.
30017988	3	54	theme	swelling	413:420	arg1	behavior					422:429	swelling behavior and biocompatibility tests	413:456	behavior	422:429	Moreover, swelling behavior and biocompatibility tests were carried out.
30017988	0	55	theme	chitosan	61:68	arg1	collagen					71:78	collagen	71:78	collagen	71:78	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	0	55	theme	chitosan	61:68	arg1	mixtures					49:56	mixtures	49:56	mixtures of chitosan	49:68	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	0	55	theme	chitosan	61:68	arg1	glycosaminoglycans					84:101	glycosaminoglycans	84:101	glycosaminoglycans	84:101	Physicochemical properties of scaffolds based on mixtures of chitosan, collagen and glycosaminoglycans with nano-hydroxyapatite addition.
30017988	1	56	theme	freeze-drying	294:306	arg1	method					308:313	the freeze-drying method	290:313	the freeze-drying method	290:313	Scaffolds based on chitosan (CTS), collagen (Coll), and glycosaminoglycans (GAGs) mixtures with nano-hydroxyapatite (HAp) were obtained with the use of the freeze-drying method.
30017988	9	57	theme	suitability	1053:1063	arg1	assessment					1029:1038	the preliminary assessment	1013:1038	the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2	1013:1116	Moreover, the preliminary assessment of scaffolds suitability for cell growth, human osteosarcoma cell line SaOS-2 was used.
30017988	7	58	theme	physicochemical	880:894	arg1	properties					896:905	The physicochemical properties	876:905	The physicochemical properties as swelling and mechanical parameters	876:943	The physicochemical properties as swelling and mechanical parameters were tested.
30017988	10	59	theme	hydroxyapatite	1179:1192	arg1	addition					1167:1174	the addition	1163:1174	the addition of hydroxyapatite	1163:1192	The obtained results indicate that the addition of hydroxyapatite improves the mechanical parameters and cells biological response of the studied materials.
30610462	8	0	theme	cell	1670:1673	arg1	encapsulation					1675:1687	cell encapsulation	1670:1687	cell encapsulation	1670:1687	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	0	1	from	effect	18:23	arg1	cytocompatibility					81:97	cytocompatibility	81:97	cytocompatibility of methyl cellulose	81:117	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	0	1	from	effect	18:23	arg1	properties					66:75	physical properties	57:75	physical properties	57:75	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	4	2	theme	embedded	859:866	arg1	BPCs					897:900	BPCs	897:900	BPCs	897:900	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	2	theme	embedded	859:866	arg1	chondrocytes					883:894	embedded bovine primary chondrocytes	859:894	embedded bovine primary chondrocytes (BPCs)	859:901	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	6	3	theme	chain	1258:1262	arg1	mobility					1264:1271	MC chain mobility	1255:1271	MC chain mobility	1255:1271	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	10	4	theme	bioengineered	1982:1994	arg1	tissue					1996:2001	bioengineered tissue	1982:2001	bioengineered tissue for cartilage regeneration	1982:2028	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	8	5	theme	MC	1545:1546	arg1	scCO2-treatment					1526:1540	The scCO2-treatment	1522:1540	The scCO2-treatment of MC	1522:1546	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	6	6	theme	permeation	1123:1132	arg1	chromatography					1134:1147	Gel permeation chromatography	1119:1147	Gel permeation chromatography analysis	1119:1156	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	3	7	theme	sterilisation	529:541	arg1	autoclave					552:560	autoclave	552:560	autoclave	552:560	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	7	theme	sterilisation	529:541	arg1	irradiation					618:628	gamma (γ) irradiation	608:628	gamma (γ) irradiation	608:628	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	7	theme	sterilisation	529:541	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	7	theme	sterilisation	529:541	arg1	UV-					600:602	UV-	600:602	UV-	600:602	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	7	theme	sterilisation	529:541	arg1	treatment					589:597	supercritical CO2 (scCO2) treatment	563:597	supercritical CO2 (scCO2) treatment	563:597	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	6	8	theme	alginate	1297:1304	arg1	network					1306:1312	the Ca2+-crosslinked alginate network	1276:1312	the Ca2+-crosslinked alginate network as well as gel composition and microstructure	1276:1358	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	0	9	theme	methyl	102:107	arg1	cellulose					109:117	methyl cellulose	102:117	methyl cellulose	102:117	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	3	10	theme	cellular	695:702	arg1	responses					704:712	cellular responses	695:712	cellular responses	695:712	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	4	11	theme	primary	875:881	arg1	BPCs					897:900	BPCs	897:900	BPCs	897:900	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	11	theme	primary	875:881	arg1	chondrocytes					883:894	embedded bovine primary chondrocytes	859:894	embedded bovine primary chondrocytes (BPCs)	859:901	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	3	12	theme	supercritical	563:575	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	12	theme	supercritical	563:575	arg1	treatment					589:597	supercritical CO2 (scCO2) treatment	563:597	supercritical CO2 (scCO2) treatment	563:597	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	13	theme	scCO2	582:586	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	13	theme	scCO2	582:586	arg1	treatment					589:597	supercritical CO2 (scCO2) treatment	563:597	supercritical CO2 (scCO2) treatment	563:597	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	4	14	theme	methyl	752:757	arg1	cellulose					759:767	methyl cellulose	752:767	methyl cellulose (MC)	752:772	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	14	theme	methyl	752:757	arg1	MC					770:771	MC	770:771	MC	770:771	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	15	theme	bovine	868:873	arg1	BPCs					897:900	BPCs	897:900	BPCs	897:900	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	15	theme	bovine	868:873	arg1	chondrocytes					883:894	embedded bovine primary chondrocytes	859:894	embedded bovine primary chondrocytes (BPCs)	859:901	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	9	16	theme	FT-IR	1714:1718	arg1	spectra					1720:1726	consistent FT-IR spectra	1703:1726	consistent FT-IR spectra	1703:1726	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	3	17	theme	CO2	577:579	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	17	theme	CO2	577:579	arg1	treatment					589:597	supercritical CO2 (scCO2) treatment	563:597	supercritical CO2 (scCO2) treatment	563:597	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	1	18	theme	biomaterial	280:290	arg1	sterility					265:273	the sterility	261:273	the sterility of a biomaterial	261:290	For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured.
30610462	7	19	theme	best	1487:1490	arg1	autoclaving					1468:1478	autoclaving	1468:1478	autoclaving	1468:1478	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	7	19	theme	best	1487:1490	arg1	UV-irradiation					1449:1462	UV-irradiation	1449:1462	UV-irradiation	1449:1462	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	7	19	theme	best	1487:1490	arg1	candidates					1492:1501	the best candidates	1483:1501	the best candidates for sterilisation	1483:1519	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	6	20	theme	Gel	1119:1121	arg1	chromatography					1134:1147	Gel permeation chromatography	1119:1147	Gel permeation chromatography analysis	1119:1156	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	7	21	theme	proteoglycan	1394:1405	arg1	production					1414:1423	proteoglycan matrix production	1394:1423	proteoglycan matrix production	1394:1423	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	6	22	theme	significant	1169:1179	arg1	reduction					1181:1189	a significant reduction	1167:1189	a significant reduction in MC molecular mass	1167:1210	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	0	23	theme	cellulose	109:117	arg1	cytocompatibility					81:97	cytocompatibility	81:97	cytocompatibility of methyl cellulose	81:117	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	0	23	theme	cellulose	109:117	arg1	properties					66:75	physical properties	57:75	physical properties	57:75	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	8	24	theme	unfavourable	1563:1574	arg1	response					1581:1588	an unfavourable cell response	1560:1588	an unfavourable cell response	1560:1588	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	4	25	theme	bioink	830:835	arg1	investigations					719:732	The investigations	715:732	The investigations	715:732	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	4	25	theme	bioink	830:835	arg1	component					779:787	a component	777:787	a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs)	777:901	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	10	26	theme	process	1963:1969	arg1	development					1893:1903	the development	1889:1903	the development of a clinically appropriate 3D-printing-based fabrication process	1889:1969	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	7	27	dep	survival	1381:1388	arg1	regard					1366:1371	regard	1366:1371	regard	1366:1371	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	5	28	theme	strong	993:998	arg1	reduction					1000:1008	a strong reduction	991:1008	a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting	991:1116	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	6	29	theme	MC	1255:1256	arg1	mobility					1264:1271	MC chain mobility	1255:1271	MC chain mobility	1255:1271	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	2	30	theme	sterilisation	325:337	arg1	techniques					339:348	common sterilisation techniques	318:348	common sterilisation techniques	318:348	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	2	30	theme	sterilisation	325:337	arg1	impact					383:388	impact	383:388	impact	383:388	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	6	31	link	Ca2+-crosslinked	1280:1295	arg1	network					1306:1312	the Ca2+-crosslinked alginate network	1276:1312	the Ca2+-crosslinked alginate network as well as gel composition and microstructure	1276:1358	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	2	32	theme	physicochemical	402:416	arg1	attributes					418:427	material physicochemical attributes	393:427	material physicochemical attributes	393:427	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	2	33	theme	tissue	467:472	arg1	engineering					474:484	tissue engineering	467:484	tissue engineering	467:484	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	9	34	theme	chemical	1729:1736	arg1	alterations					1738:1748	chemical alterations	1729:1748	chemical alterations	1729:1748	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	9	34	theme	chemical	1729:1736	arg1	cause					1773:1777	a cause	1771:1777	a cause for the differences seen between MC treatments on alg/MC behaviour	1771:1844	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	2	35	theme	common	318:323	arg1	techniques					339:348	common sterilisation techniques	318:348	common sterilisation techniques	318:348	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	2	35	theme	common	318:323	arg1	impact					383:388	impact	383:388	impact	383:388	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	5	36	from	reduction	1000:1008	arg1	stability					1034:1042	stability	1034:1042	stability	1034:1042	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	5	36	from	reduction	1000:1008	arg1	viscosity					1020:1028	alg/MC viscosity	1013:1028	alg/MC viscosity	1013:1028	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	2	37	theme	material	393:400	arg1	attributes					418:427	material physicochemical attributes	393:427	material physicochemical attributes	393:427	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	7	38	theme	matrix	1407:1412	arg1	production					1414:1423	proteoglycan matrix production	1394:1423	proteoglycan matrix production	1394:1423	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	6	39	theme	molecular	1197:1205	arg1	mass					1207:1210	MC molecular mass	1194:1210	MC molecular mass	1194:1210	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	2	40	from	impact	383:388	arg1	attributes					418:427	material physicochemical attributes	393:427	material physicochemical attributes	393:427	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	0	41	dep	properties	66:75	arg1	the					53:55	the	53:55	the	53:55	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	9	42	from	treatments	1815:1824	arg1	behaviour					1836:1844	alg/MC behaviour	1829:1844	alg/MC behaviour	1829:1844	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	2	43	dep	intense	354:360	arg1	techniques					339:348	common sterilisation techniques	318:348	common sterilisation techniques	318:348	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	2	43	dep	intense	354:360	arg1	impact					383:388	impact	383:388	impact	383:388	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	0	44	theme	methods	42:48	arg1	effect					18:23	the effect	14:23	the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes	14:186	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	5	45	theme	alg/MC	1013:1018	arg1	viscosity					1020:1028	alg/MC viscosity	1013:1028	alg/MC viscosity	1013:1028	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	8	46	theme	careful	1624:1630	arg1	optimisation					1632:1643	careful optimisation	1624:1643	careful optimisation prior to application for cell encapsulation	1624:1687	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	1	47	theme	cells	219:223	arg1	applications					248:259	future therapeutic applications	229:259	future therapeutic applications	229:259	For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured.
30610462	1	47	theme	cells	219:223	arg1	incorporation					202:214	the incorporation	198:214	the incorporation of cells	198:223	For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured.
30610462	0	48	theme	sterilisation	28:40	arg1	methods					42:48	sterilisation methods	28:48	sterilisation methods	28:48	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	6	49	theme	Ca2+-crosslinked	1280:1295	arg1	network					1306:1312	the Ca2+-crosslinked alginate network	1276:1312	the Ca2+-crosslinked alginate network as well as gel composition and microstructure	1276:1358	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	6	50	theme	gel	1325:1327	arg1	composition					1329:1339	gel composition	1325:1339	gel composition	1325:1339	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	3	51	theme	gamma	608:612	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	51	theme	gamma	608:612	arg1	irradiation					618:628	gamma (γ) irradiation	608:628	gamma (γ) irradiation	608:628	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	9	52	theme	alg/MC	1829:1834	arg1	behaviour					1836:1844	alg/MC behaviour	1829:1844	alg/MC behaviour	1829:1844	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	2	53	theme	3D	490:491	arg1	printing					493:500	3D printing	490:500	3D printing	490:500	However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing.
30610462	9	54	theme	MC	1812:1813	arg1	treatments					1815:1824	MC treatments	1812:1824	MC treatments on alg/MC behaviour	1812:1844	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	8	55	theme	cell	1576:1579	arg1	response					1581:1588	an unfavourable cell response	1560:1588	an unfavourable cell response	1560:1588	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	7	56	theme	cell	1376:1379	arg1	survival					1381:1388	cell survival	1376:1388	cell survival	1376:1388	With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation.
30610462	10	57	theme	cartilage	2007:2015	arg1	regeneration					2017:2028	cartilage regeneration	2007:2028	cartilage regeneration	2007:2028	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	3	58	theme	present	510:516	arg1	study					518:522	the present study	506:522	the present study	506:522	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	5	59	dep	autoclave	923:931	arg1	contrast					907:914	contrast	907:914	contrast	907:914	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	3	60	theme	material	671:678	arg1	properties					680:689	material properties	671:689	material properties	671:689	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	4	61	theme	alg/MC	822:827	arg1	bioink					830:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	9	62	theme	consistent	1703:1712	arg1	spectra					1720:1726	consistent FT-IR spectra	1703:1726	consistent FT-IR spectra	1703:1726	As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC treatments on alg/MC behaviour.
30610462	6	63	theme	MC	1194:1195	arg1	mass					1207:1210	MC molecular mass	1194:1210	MC molecular mass	1194:1210	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	0	64	theme	chondrocytes	175:186	arg1	3D-bioplotting					157:170	3D-bioplotting	157:170	3D-bioplotting of chondrocytes	157:186	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	10	65	theme	fabrication	1951:1961	arg1	process					1963:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	5	66	theme	γ-irradiated	963:974	arg1	MC					976:977	the γ-irradiated MC	959:977	the γ-irradiated MC	959:977	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	4	67	theme	cellulose	811:819	arg1	bioink					830:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	6	68	theme	chromatography	1134:1147	arg1	analysis					1149:1156	Gel permeation chromatography analysis	1119:1156	Gel permeation chromatography analysis	1119:1156	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
30610462	1	69	theme	future	229:234	arg1	applications					248:259	future therapeutic applications	229:259	future therapeutic applications	229:259	For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured.
30610462	10	70	theme	3D-printing-based	1933:1949	arg1	process					1963:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	0	71	with	combination	127:137	arg1	alginate					144:151	alginate	144:151	alginate	144:151	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	1	72	theme	therapeutic	236:246	arg1	applications					248:259	future therapeutic applications	229:259	future therapeutic applications	229:259	For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured.
30610462	3	73	from	impact	661:666	arg1	responses					704:712	cellular responses	695:712	cellular responses	695:712	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	73	from	impact	661:666	arg1	properties					680:689	material properties	671:689	material properties	671:689	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	10	74	theme	appropriate	1921:1931	arg1	process					1963:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	a clinically appropriate 3D-printing-based fabrication process	1908:1969	This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.
30610462	4	75	theme	alginate/methyl	795:809	arg1	bioink					830:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	an alginate/methyl cellulose (alg/MC) bioink	792:835	The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs).
30610462	0	76	theme	physical	57:64	arg1	properties					66:75	physical properties	57:75	physical properties	57:75	Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes.
30610462	5	77	theme	precise	1098:1104	arg1	bioprinting					1106:1116	precise bioprinting	1098:1116	precise bioprinting	1098:1116	In contrast to the autoclave, scCO2 and UV-treatments, the γ-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting.
30610462	3	78	theme	γ	615:615	arg1	methods					543:549	four sterilisation methods	524:549	four sterilisation methods	524:549	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	3	78	theme	γ	615:615	arg1	irradiation					618:628	gamma (γ) irradiation	608:628	gamma (γ) irradiation	608:628	In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV- and gamma (γ) irradiation were evaluated regarding their impact on material properties and cellular responses.
30610462	8	79	theme	prior	1645:1649	arg1	optimisation					1632:1643	careful optimisation	1624:1643	careful optimisation prior to application for cell encapsulation	1624:1687	The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation.
30610462	6	80	from	reduction	1181:1189	arg1	mass					1207:1210	MC molecular mass	1194:1210	MC molecular mass	1194:1210	Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after γ-irradiation, which influenced MC chain mobility in the Ca2+-crosslinked alginate network as well as gel composition and microstructure.
31369786	6	0	theme	storage	874:880	arg1	G					891:891	G'	891:892	G'	891:892	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	6	0	theme	storage	874:880	arg1	modulus					882:888	a storage modulus	872:888	a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa	872:919	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	0	1	theme	composites	155:164	arg1	properties					113:122	mechanical properties	102:122	mechanical properties of poly(acrylic acid)/β-chitin composites	102:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	6	2	theme	%	777:777	arg1	suspensions					786:796	0.1% chitin suspensions	774:796	0.1% chitin suspensions exfoliated in AscA and AcrA solutions	774:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	2	3	theme	β-chitin	330:337	arg1	fibrils					339:345	β-chitin fibrils	330:345	β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA)	330:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	1	4	theme	mechanical	244:253	arg1	properties					255:264	their mechanical properties	238:264	their mechanical properties	238:264	Chitin whiskers are promising reinforcing filler for composites due to their mechanical properties, renewable nature and low cost.
31369786	6	5	theme	gel-like	849:856	arg1	behavior					858:865	similar gel-like behavior	841:865	similar gel-like behavior	841:865	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	2	6	from	preparation	315:325	arg1	pens					358:361	squid pens	352:361	squid pens in ascorbic acid aqueous solutions (AscA)	352:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	4	7	theme	aqueous	622:628	arg1	solution					630:637	acrylic acid (AcrA) aqueous solution	602:637	acrylic acid (AcrA) aqueous solution	602:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	8	8	theme	composites	1182:1191	arg1	properties					1164:1173	Mechanical properties	1153:1173	Mechanical properties of the composites	1153:1191	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	9	9	theme	maximum	1356:1362	arg1	effect					1376:1381	the maximum reinforcing effect	1352:1381	the maximum reinforcing effect	1352:1381	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	4	10	theme	acid	610:613	arg1	solution					630:637	acrylic acid (AcrA) aqueous solution	602:637	acrylic acid (AcrA) aqueous solution	602:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	4	11	theme	AcrA	616:619	arg1	solution					630:637	acrylic acid (AcrA) aqueous solution	602:637	acrylic acid (AcrA) aqueous solution	602:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	5	12	theme	TEMPO-oxidized	671:684	arg1	fibrils					695:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	5	12	theme	TEMPO-oxidized	671:684	arg1	sample					730:735	a reference sample	718:735	a reference sample for comparison of different methods	718:771	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	7	13	theme	1-3 wt	1082:1087	arg1	%					1088:1088	1-3 wt%	1082:1088	1-3 wt% of β-chitin fibrils	1082:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	7	13	theme	1-3 wt	1082:1087	arg1	fibrils					1102:1108	β-chitin fibrils	1093:1108	β-chitin fibrils	1093:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	8	14	theme	Mechanical	1153:1162	arg1	properties					1164:1173	Mechanical properties	1153:1173	Mechanical properties of the composites	1153:1191	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	3	15	with	solution	436:443	arg1	AscA					456:459	2 g/l AscA	450:459	2 g/l AscA	450:459	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	6	16	from	exfoliated	798:807	arg1	solutions					826:834	AscA and AcrA solutions	812:834	AscA and AcrA solutions	812:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	0	17	theme	acrylic	132:138	arg1	poly					127:130	poly	127:130	poly(acrylic acid)/β-chitin composites	127:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	0	17	theme	acrylic	132:138	arg1	acid					140:143	acrylic acid	132:143	acrylic acid	132:143	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	9	18	theme	high	1398:1401	arg1	temperature					1403:1413	high temperature	1398:1413	high temperature (>120 °C)	1398:1423	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	9	18	theme	high	1398:1401	arg1	>120 °C					1416:1422	>120 °C	1416:1422	>120 °C	1416:1422	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	8	19	theme	temperature	1231:1241	arg1	range					1243:1247	the temperature range	1227:1247	the temperature range of 25-250 °C	1227:1260	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	0	20	theme	β-chitin	55:62	arg1	fibrils					64:70	β-chitin fibrils	55:70	β-chitin fibrils	55:70	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	9	21	theme	high	1429:1432	arg1	RH					1459:1460	≥75.3%RH	1453:1460	≥75.3%RH	1453:1460	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	9	21	theme	high	1429:1432	arg1	humidity					1443:1450	high relative humidity	1429:1450	high relative humidity (≥75.3%RH)	1429:1461	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	0	22	from	Effect	0:5	arg1	properties					113:122	mechanical properties	102:122	mechanical properties of poly(acrylic acid)/β-chitin composites	102:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	0	22	from	Effect	0:5	arg1	behavior					43:50	rheological behavior	31:50	rheological behavior of β-chitin fibrils in aqueous suspensions	31:93	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	7	23	theme	polymerization	1129:1142	arg1	filling					1144:1150	polymerization filling	1129:1150	polymerization filling	1129:1150	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	0	24	from	behavior	43:50	arg1	suspensions					83:93	aqueous suspensions	75:93	aqueous suspensions	75:93	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	2	25	theme	acid	375:378	arg1	solutions					388:396	ascorbic acid aqueous solutions	366:396	ascorbic acid aqueous solutions (AscA)	366:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	2	25	theme	acid	375:378	arg1	AscA					399:402	AscA	399:402	AscA	399:402	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	4	26	dep	found	644:648	arg1	0.75-2 g/l					651:660	0.75-2 g/l	651:660	0.75-2 g/l	651:660	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	4	26	dep	found	644:648	arg1	3-4 h					663:667	3-4 h	663:667	3-4 h	663:667	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	6	27	theme	fibrils	997:1003	arg1	dispersion					955:964	0.1% dispersion	950:964	0.1% dispersion of the TEMPO-oxidized β-chitin fibrils	950:1003	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	7	28	theme	β-chitin	1093:1100	arg1	fibrils					1102:1108	β-chitin fibrils	1093:1108	β-chitin fibrils	1093:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	1	29	theme	renewable	267:275	arg1	nature					277:282	renewable nature	267:282	renewable nature	267:282	Chitin whiskers are promising reinforcing filler for composites due to their mechanical properties, renewable nature and low cost.
31369786	6	30	theme	TEMPO-oxidized	973:986	arg1	fibrils					997:1003	the TEMPO-oxidized β-chitin fibrils	969:1003	the TEMPO-oxidized β-chitin fibrils	969:1003	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	0	31	theme	exfoliating	10:20	arg1	agent					22:26	exfoliating agent	10:26	exfoliating agent	10:26	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	6	32	theme	%	953:953	arg1	dispersion					955:964	0.1% dispersion	950:964	0.1% dispersion of the TEMPO-oxidized β-chitin fibrils	950:1003	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	8	33	theme	100	1325:1327	arg1	%					1320:1320	%	1320:1320	%	1320:1320	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	5	34	theme	different	755:763	arg1	methods					765:771	different methods	755:771	different methods	755:771	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	6	35	dep	show	836:839	arg1	whereas					936:942	whereas	936:942	whereas	936:942	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	2	36	theme	squid	352:356	arg1	pens					358:361	squid pens	352:361	squid pens in ascorbic acid aqueous solutions (AscA)	352:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	1	37	theme	due	231:233	arg1	composites					220:229	composites	220:229	composites due to their mechanical properties, renewable nature and low cost	220:295	Chitin whiskers are promising reinforcing filler for composites due to their mechanical properties, renewable nature and low cost.
31369786	7	38	theme	fibrils	1102:1108	arg1	%					1088:1088	1-3 wt%	1082:1088	1-3 wt% of β-chitin fibrils	1082:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	7	38	theme	fibrils	1102:1108	arg1	fibrils					1102:1108	β-chitin fibrils	1093:1108	β-chitin fibrils	1093:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	4	39	theme	exfoliation	587:597	arg1	conditions					564:573	The optimal conditions	552:573	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution	552:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	5	40	theme	reference	720:728	arg1	fibrils					695:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	5	40	theme	reference	720:728	arg1	sample					730:735	a reference sample	718:735	a reference sample for comparison of different methods	718:771	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	2	41	theme	fibrils	339:345	arg1	preparation					315:325	preparation	315:325	preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA)	315:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	6	42	theme	0.1	774:776	arg1	%					777:777	%	777:777	%	777:777	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	3	43	theme	individualized	501:514	arg1	fibrils					516:522	individualized fibrils	501:522	individualized fibrils with an aspect ratio > 250	501:549	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	2	44	theme	new	300:302	arg1	method					304:309	A new method	298:309	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA)	298:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	4	45	theme	optimal	556:562	arg1	conditions					564:573	The optimal conditions	552:573	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution	552:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	3	46	with	fibrils	516:522	arg1	ratio > 250					539:549	an aspect ratio > 250	529:549	an aspect ratio > 250	529:549	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	6	47	theme	similar	841:847	arg1	behavior					858:865	similar gel-like behavior	841:865	similar gel-like behavior	841:865	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	3	48	theme	aspect	532:537	arg1	ratio > 250					539:549	an aspect ratio > 250	529:549	an aspect ratio > 250	529:549	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	1	49	theme	Chitin	167:172	arg1	whiskers					174:181	Chitin whiskers	167:181	Chitin whiskers	167:181	Chitin whiskers are promising reinforcing filler for composites due to their mechanical properties, renewable nature and low cost.
31369786	6	50	theme	34 ± 12 Pa	910:919	arg1	G					891:891	G'	891:892	G'	891:892	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	6	50	theme	34 ± 12 Pa	910:919	arg1	modulus					882:888	a storage modulus	872:888	a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa	872:919	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	6	51	from	solutions	826:834	arg1	exfoliated					798:807	exfoliated	798:807	exfoliated	798:807	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	9	52	theme	reinforcing	1364:1374	arg1	effect					1376:1381	the maximum reinforcing effect	1352:1381	the maximum reinforcing effect	1352:1381	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	6	53	theme	98 ± 23	898:904	arg1	G					891:891	G'	891:892	G'	891:892	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	6	53	theme	98 ± 23	898:904	arg1	modulus					882:888	a storage modulus	872:888	a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa	872:919	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	4	54	theme	acrylic	602:608	arg1	solution					630:637	acrylic acid (AcrA) aqueous solution	602:637	acrylic acid (AcrA) aqueous solution	602:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	8	55	from	%	1328:1328	arg1	humidity					1289:1296	controlled relative humidity	1269:1296	controlled relative humidity in the range from 53.6% to 100%	1269:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	8	55	from	%	1328:1328	arg1	range					1305:1309	the range	1301:1309	the range from 53.6% to 100%	1301:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	8	56	theme	dry	1214:1216	arg1	state					1218:1222	dry state	1214:1222	dry state	1214:1222	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	5	57	theme	β-chitin	686:693	arg1	fibrils					695:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils	671:701	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	5	57	theme	β-chitin	686:693	arg1	sample					730:735	a reference sample	718:735	a reference sample for comparison of different methods	718:771	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	0	58	theme	/β-chitin	145:153	arg1	composites					155:164	poly(acrylic acid)/β-chitin composites	127:164	poly(acrylic acid)/β-chitin composites	127:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	6	59	theme	0.1	950:952	arg1	%					953:953	%	953:953	%	953:953	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	8	60	theme	controlled	1269:1278	arg1	humidity					1289:1296	controlled relative humidity	1269:1296	controlled relative humidity in the range from 53.6% to 100%	1269:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	0	61	theme	fibrils	64:70	arg1	behavior					43:50	rheological behavior	31:50	rheological behavior of β-chitin fibrils in aqueous suspensions	31:93	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	0	62	theme	aqueous	75:81	arg1	suspensions					83:93	aqueous suspensions	75:93	aqueous suspensions	75:93	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	8	63	dep	%	1320:1320	arg1	to					1322:1323	to	1322:1323	to	1322:1323	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	8	64	theme	25-250 °C	1252:1260	arg1	range					1243:1247	the temperature range	1227:1247	the temperature range of 25-250 °C	1227:1260	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	4	65	from	conditions	564:573	arg1	solution					630:637	acrylic acid (AcrA) aqueous solution	602:637	acrylic acid (AcrA) aqueous solution	602:637	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	9	66	theme	relative	1434:1441	arg1	RH					1459:1460	≥75.3%RH	1453:1460	≥75.3%RH	1453:1460	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	9	66	theme	relative	1434:1441	arg1	humidity					1443:1450	high relative humidity	1429:1450	high relative humidity (≥75.3%RH)	1429:1461	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	0	67	theme	mechanical	102:111	arg1	properties					113:122	mechanical properties	102:122	mechanical properties of poly(acrylic acid)/β-chitin composites	102:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	8	68	theme	relative	1280:1287	arg1	humidity					1289:1296	controlled relative humidity	1269:1296	controlled relative humidity in the range from 53.6% to 100%	1269:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	2	69	theme	ascorbic	366:373	arg1	acid					375:378	ascorbic acid	366:378	ascorbic acid aqueous solutions (AscA)	366:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	5	70	theme	methods	765:771	arg1	comparison					741:750	comparison	741:750	comparison of different methods	741:771	TEMPO-oxidized β-chitin fibrils were chosen as a reference sample for comparison of different methods.
31369786	9	71	theme	%	1458:1458	arg1	RH					1459:1460	≥75.3%RH	1453:1460	≥75.3%RH	1453:1460	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	9	71	theme	%	1458:1458	arg1	humidity					1443:1450	high relative humidity	1429:1450	high relative humidity (≥75.3%RH)	1429:1461	It was revealed that the maximum reinforcing effect is achieved at high temperature (>120 °C) and high relative humidity (≥75.3%RH).
31369786	6	72	theme	β-chitin	988:995	arg1	fibrils					997:1003	the TEMPO-oxidized β-chitin fibrils	969:1003	the TEMPO-oxidized β-chitin fibrils	969:1003	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	0	73	theme	agent	22:26	arg1	Effect					0:5	Effect	0:5	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.	0:165	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	3	74	theme	solution	436:443	arg1	Stirring					420:427	Stirring	420:427	Stirring of the solution with 2 g/l AscA for 2 h	420:467	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	0	75	theme	rheological	31:41	arg1	behavior					43:50	rheological behavior	31:50	rheological behavior of β-chitin fibrils in aqueous suspensions	31:93	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	1	76	theme	low	288:290	arg1	cost					292:295	low cost	288:295	low cost	288:295	Chitin whiskers are promising reinforcing filler for composites due to their mechanical properties, renewable nature and low cost.
31369786	6	77	theme	AcrA	821:824	arg1	solutions					826:834	AscA and AcrA solutions	812:834	AscA and AcrA solutions	812:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	6	78	theme	dispersion	955:964	arg1	G					944:944	G'	944:945	G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils	944:1003	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	8	79	theme	53.6	1316:1319	arg1	%					1320:1320	%	1320:1320	%	1320:1320	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	2	80	theme	aqueous	380:386	arg1	solutions					388:396	ascorbic acid aqueous solutions	366:396	ascorbic acid aqueous solutions (AscA)	366:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	2	80	theme	aqueous	380:386	arg1	AscA					399:402	AscA	399:402	AscA	399:402	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	7	81	theme	acrylic	1057:1063	arg1	poly					1052:1055	poly	1052:1055	poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils	1052:1108	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	7	81	theme	acrylic	1057:1063	arg1	acid					1065:1068	acrylic acid	1057:1068	acrylic acid	1057:1068	Composites based on poly(acrylic acid) comprising 1-3 wt% of β-chitin fibrils were fabricated by polymerization filling.
31369786	6	82	theme	AscA	812:815	arg1	solutions					826:834	AscA and AcrA solutions	812:834	AscA and AcrA solutions	812:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	2	83	from	pens	358:361	arg1	preparation					315:325	preparation	315:325	preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA)	315:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	2	83	from	pens	358:361	arg1	fibrils					339:345	β-chitin fibrils	330:345	β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA)	330:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	2	83	from	pens	358:361	arg1	AscA					399:402	AscA	399:402	AscA	399:402	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	2	83	from	pens	358:361	arg1	solutions					388:396	ascorbic acid aqueous solutions	366:396	ascorbic acid aqueous solutions (AscA)	366:403	A new method for preparation of β-chitin fibrils from squid pens in ascorbic acid aqueous solutions (AscA) was developed.
31369786	0	84	theme	poly	127:130	arg1	composites					155:164	poly(acrylic acid)/β-chitin composites	127:164	poly(acrylic acid)/β-chitin composites	127:164	Effect of exfoliating agent on rheological behavior of β-chitin fibrils in aqueous suspensions and on mechanical properties of poly(acrylic acid)/β-chitin composites.
31369786	8	85	from	humidity	1289:1296	arg1	range					1305:1309	the range	1301:1309	the range from 53.6% to 100%	1301:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	8	85	from	humidity	1289:1296	arg1	%					1328:1328	53.6% to 100%	1316:1328	53.6% to 100%	1316:1328	Mechanical properties of the composites were investigated in dry state in the temperature range of 25-250 °C and at controlled relative humidity in the range from 53.6% to 100%.
31369786	6	86	theme	exfoliated	798:807	arg1	suspensions					786:796	0.1% chitin suspensions	774:796	0.1% chitin suspensions exfoliated in AscA and AcrA solutions	774:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	4	87	theme	β-chitin	578:585	arg1	exfoliation					587:597	β-chitin exfoliation	578:597	β-chitin exfoliation	578:597	The optimal conditions of β-chitin exfoliation in acrylic acid (AcrA) aqueous solution were found (0.75-2 g/l, 3-4 h).
31369786	3	88	theme	2 g/l	450:454	arg1	AscA					456:459	2 g/l AscA	450:459	2 g/l AscA	450:459	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
31369786	6	89	theme	chitin	779:784	arg1	suspensions					786:796	0.1% chitin suspensions	774:796	0.1% chitin suspensions exfoliated in AscA and AcrA solutions	774:834	0.1% chitin suspensions exfoliated in AscA and AcrA solutions show similar gel-like behavior with a storage modulus (G') of 98 ± 23 and 34 ± 12 Pa, respectively, whereas G' of 0.1% dispersion of the TEMPO-oxidized β-chitin fibrils was 0.015 ± 0.005 Pa only.
31369786	3	90	theme	swollen	483:489	arg1	chitin					491:496	the swollen chitin	479:496	the swollen chitin	479:496	Stirring of the solution with 2 g/l AscA for 2 h disperses the swollen chitin to individualized fibrils with an aspect ratio > 250.
30325500	8	0	theme	lowest	1568:1573	arg1	WVP					1575:1577	the lowest WVP	1564:1577	the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 )	1564:1611	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	0	theme	lowest	1568:1573	arg1	·Pa-1					1605:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	10	1	theme	Nano-titanium	1917:1929	arg1	dioxide					1931:1937	Nano-titanium dioxide	1917:1937	Nano-titanium dioxide (TiO2 ) nanoparticles	1917:1959	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	10	1	theme	Nano-titanium	1917:1929	arg1	TiO2					1940:1943	TiO2	1940:1943	TiO2	1940:1943	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	1	2	theme	Nano-titanium	138:150	arg1	TiO2					161:164	TiO2	161:164	TiO2	161:164	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	2	theme	Nano-titanium	138:150	arg1	dioxide					152:158	Nano-titanium dioxide	138:158	Nano-titanium dioxide (TiO2 )	138:166	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	11	3	theme	Modified	2138:2145	arg1	nanoparticles					2152:2164	Modified TiO2 nanoparticles	2138:2164	Modified TiO2 nanoparticles	2138:2164	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	4	theme	×	1585:1585	arg1	WVP					1575:1577	the lowest WVP	1564:1577	the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 )	1564:1611	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	4	theme	×	1585:1585	arg1	·Pa-1					1605:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	1	5	theme	surfactant	190:199	arg1	SL					217:218	SL	217:218	SL	217:218	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	5	theme	surfactant	190:199	arg1	laurate					208:214	the surfactant sodium laurate	186:214	the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices	186:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	8	6	theme	ADPS/CS	1704:1710	arg1	film					1712:1715	the pure ADPS/CS film	1695:1715	the pure ADPS/CS film	1695:1715	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	4	7	from	increase	669:676	arg1	%					715:715	5% to 15%	707:715	5% to 15%	707:715	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	4	7	from	increase	669:676	arg1	concentration					688:700	the SL concentration	681:700	the SL concentration from 5% to 15%	681:715	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	10	8	theme	TiO2	2098:2101	arg1	nanoparticles					2103:2115	the TiO2 nanoparticles	2094:2115	the TiO2 nanoparticles	2094:2115	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	10	9	dep	APPLICATION	1904:1914	arg1	modified					1966:1973	modified	1966:1973	were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices	1961:2135	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	9	10	theme	SL	1743:1744	arg1	modification					1746:1757	SL modification	1743:1757	SL modification	1743:1757	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	8	11	dep	TiO2	1295:1298	arg1	TiO2					1307:1310	TiO2	1307:1310	TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film	1307:1356	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	11	12	theme	blend	2229:2233	arg1	films					2235:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	13	theme	highest	1373:1379	arg1	higher					1414:1419	higher	1414:1419	higher	1414:1419	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	13	theme	highest	1373:1379	arg1	TS					1381:1382	the highest TS	1369:1382	the highest TS (31.50 MPa)	1369:1394	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	13	theme	highest	1373:1379	arg1	MPa					1391:1393	31.50 MPa	1385:1393	31.50 MPa	1385:1393	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	11	14	theme	water	2313:2317	arg1	solubility					2319:2328	the water solubility	2309:2328	the water solubility	2309:2328	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	5	15	theme	phosphate/chitosan	902:919	arg1	matrices					939:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	7	16	from	break	1192:1196	arg1	elongation					1178:1187	the elongation	1174:1187	the elongation at break (E), water vapor permeability (WVP), and solubility	1174:1248	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	9	17	theme	ultrasonic	1774:1783	arg1	technology					1804:1813	ultrasonic microwave-assisted technology	1774:1813	ultrasonic microwave-assisted technology	1774:1813	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	4	18	theme	-SLx	744:747	arg1	particles					749:757	TiO2 -SLx particles	739:757	TiO2 -SLx particles	739:757	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	10	19	theme	sodium	2004:2009	arg1	laurate					2011:2017	the anionic surfactant sodium laurate	1981:2017	the anionic surfactant sodium laurate	1981:2017	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	5	20	theme	ADPS/CS	922:928	arg1	matrices					939:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	11	21	theme	packaging	2424:2432	arg1	applications					2434:2445	packaging applications	2424:2445	packaging applications	2424:2445	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	9	22	theme	technology	1804:1813	arg1	modification					1746:1757	SL modification	1743:1757	SL modification	1743:1757	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	9	22	theme	technology	1804:1813	arg1	use					1767:1769	the use	1763:1769	the use of ultrasonic microwave-assisted technology	1763:1813	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	9	22	theme	technology	1804:1813	arg1	promising					1819:1827	promising	1819:1827	promising	1819:1827	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	4	23	theme	5	707:707	arg1	%					708:708	%	708:708	%	708:708	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	0	24	theme	Phosphate/Chitosan	20:37	arg1	Films					39:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	11	25	theme	composite	2366:2374	arg1	film					2376:2379	the composite film	2362:2379	the composite film	2362:2379	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	10	26	theme	assisted	2044:2051	arg1	technology					2053:2062	ultrasonic-microwave assisted technology	2023:2062	ultrasonic-microwave assisted technology	2023:2062	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	8	27	theme	composite	1269:1277	arg1	film					1279:1282	The composite film	1265:1282	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film)	1265:1357	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	5	28	theme	acetylated	882:891	arg1	matrices					939:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	6	29	theme	unmodified	990:999	arg1	TiO2					1001:1004	unmodified TiO2	990:1004	unmodified TiO2	990:1004	Relative to unmodified TiO2 , TiO2 -SLx exhibited a better dispersion capability.
30325500	2	30	theme	TiO2	539:542	arg1	TiO2					533:536	SL-modified TiO2	521:536	SL-modified TiO2 (TiO2 -SLx)	521:548	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	2	30	theme	TiO2	539:542	arg1	-SLx					544:547	TiO2 -SLx	539:547	TiO2 -SLx	539:547	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	11	31	theme	vapor	2340:2344	arg1	permeability					2346:2357	water vapor permeability	2334:2357	water vapor permeability	2334:2357	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	0	32	theme	Sodium	108:113	arg1	Concentration					123:135	Sodium Laurate Concentration	108:135	Sodium Laurate Concentration	108:135	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	4	33	dep	%	708:708	arg1	to					710:711	to	710:711	to	710:711	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	10	34	from	dispersion	2080:2089	arg1	matrices					2128:2135	polymer matrices	2120:2135	polymer matrices	2120:2135	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	6	35	theme	dispersion	1037:1046	arg1	capability					1048:1057	a better dispersion capability	1028:1057	a better dispersion capability	1028:1057	Relative to unmodified TiO2 , TiO2 -SLx exhibited a better dispersion capability.
30325500	0	36	theme	Concentration	123:135	arg1	Effects					97:103	Effects	97:103	Effects of Sodium Laurate Concentration	97:135	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	10	37	theme	nanoparticles	2103:2115	arg1	dispersion					2080:2089	the dispersion	2076:2089	the dispersion of the TiO2 nanoparticles in polymer matrices	2076:2135	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	2	38	dep	transform	344:352	arg1	infrared					354:361	infrared	354:361	transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses	344:436	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	2	39	theme	scanning	400:407	arg1	microscopy					418:427	scanning electron microscopy	400:427	scanning electron microscopy	400:427	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	0	40	theme	Acetylated	0:9	arg1	Films					39:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	1	41	theme	TiO2	295:298	arg1	dispersion					281:290	the dispersion	277:290	the dispersion of TiO2 in polymer matrices	277:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	5	42	theme	blended	931:937	arg1	matrices					939:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	11	43	theme	composite	2278:2286	arg1	film					2288:2291	the composite film	2274:2291	the composite film	2274:2291	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	2	44	dep	spectroscopy	363:374	arg1	analyses					429:436	analyses	429:436	analyses	429:436	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	7	45	theme	composite	1141:1149	arg1	films					1151:1155	the composite films	1137:1155	the composite films	1137:1155	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	1	46	theme	polymer	303:309	arg1	matrices					311:318	polymer matrices	303:318	polymer matrices	303:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	9	47	mod	modification	1746:1757	arg1	technology					1804:1813	ultrasonic microwave-assisted technology	1774:1813	ultrasonic microwave-assisted technology	1774:1813	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	9	47	mod	modification	1746:1757	arg3	SL					1743:1744	SL modification	1743:1757	SL modification	1743:1757	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	2	48	theme	chemical	490:497	arg1	bonding					499:505	chemical bonding	490:505	chemical bonding	490:505	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	8	49	theme	pure	1438:1441	arg1	film					1451:1454	the pure ADPS/CS film	1434:1454	the pure ADPS/CS film	1434:1454	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	50	theme	%	1328:1328	arg1	SL					1330:1331	15% SL	1326:1331	15% SL	1326:1331	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	0	51	theme	Sodium	61:66	arg1	Nano-TiO2					85:93	Sodium Laurate-Modified Nano-TiO2	61:93	Sodium Laurate-Modified Nano-TiO2	61:93	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	0	52	dep	Reinforced	45:54	arg1	Effects					97:103	Effects	97:103	Effects of Sodium Laurate Concentration	97:135	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	7	53	from	solubility	1239:1248	arg1	elongation					1178:1187	the elongation	1174:1187	the elongation at break (E), water vapor permeability (WVP), and solubility	1174:1248	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	11	54	theme	di-starch	2200:2208	arg1	films					2235:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	55	theme	-SL15	1347:1351	arg1	film					1353:1356	ADPS/CS-TiO2 -SL15 film	1334:1356	TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film	1307:1356	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	1	56	theme	ultrasonic	225:234	arg1	technology					255:264	ultrasonic microwave-assisted technology	225:264	ultrasonic microwave-assisted technology	225:264	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	5	57	theme	TiO2	822:825	arg1	%					851:851	3 wt%	847:851	3 wt%	847:851	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	5	57	theme	TiO2	822:825	arg1	nanoparticles					832:844	TiO2 -SLx nanoparticles	822:844	TiO2 -SLx nanoparticles (3 wt%)	822:852	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	10	58	theme	PRACTICAL	1894:1902	arg1	APPLICATION					1904:1914	PRACTICAL APPLICATION	1894:1914	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.	1894:2136	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	8	59	theme	ADPS/CS-TiO2	1469:1480	arg1	film					1488:1491	the ADPS/CS-TiO2 -SL25 film	1465:1491	the ADPS/CS-TiO2 -SL25 film	1465:1491	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	60	theme	ADPS/CS-TiO2	1530:1541	arg1	film					1549:1552	the ADPS/CS-TiO2 -SL15 film	1526:1552	the ADPS/CS-TiO2 -SL15 film	1526:1552	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	7	61	theme	vapor	1209:1213	arg1	WVP					1229:1231	WVP	1229:1231	WVP	1229:1231	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	7	61	theme	vapor	1209:1213	arg1	permeability					1215:1226	water vapor permeability	1203:1226	water vapor permeability (WVP)	1203:1232	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	7	62	theme	tensile	1112:1118	arg1	TS					1130:1131	TS	1130:1131	TS	1130:1131	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	7	62	theme	tensile	1112:1118	arg1	strength					1120:1127	the tensile strength	1108:1127	the tensile strength (TS) of the composite films	1108:1155	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	2	63	theme	X-ray	377:381	arg1	diffraction					383:393	X-ray diffraction	377:393	X-ray diffraction	377:393	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	10	64	theme	dioxide	1931:1937	arg1	nanoparticles					1947:1959	Nano-titanium dioxide (TiO2 ) nanoparticles	1917:1959	Nano-titanium dioxide (TiO2 ) nanoparticles	1917:1959	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	10	65	theme	polymer	2120:2126	arg1	matrices					2128:2135	polymer matrices	2120:2135	polymer matrices	2120:2135	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	8	66	theme	pure	1699:1702	arg1	film					1712:1715	the pure ADPS/CS film	1695:1715	the pure ADPS/CS film	1695:1715	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	67	dep	TiO2	1307:1310	arg1	film					1353:1356	ADPS/CS-TiO2 -SL15 film	1334:1356	TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film	1307:1356	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	5	68	theme	wt	849:850	arg1	%					851:851	3 wt%	847:851	3 wt%	847:851	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	5	68	theme	wt	849:850	arg1	nanoparticles					832:844	TiO2 -SLx nanoparticles	822:844	TiO2 -SLx nanoparticles (3 wt%)	822:852	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	7	69	from	permeability	1215:1226	arg1	elongation					1178:1187	the elongation	1174:1187	the elongation at break (E), water vapor permeability (WVP), and solubility	1174:1248	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	1	70	theme	sodium	201:206	arg1	SL					217:218	SL	217:218	SL	217:218	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	70	theme	sodium	201:206	arg1	laurate					208:214	the surfactant sodium laurate	186:214	the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices	186:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	7	71	theme	SL	1080:1081	arg1	concentration					1083:1095	the SL concentration	1076:1095	the SL concentration	1076:1095	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	10	72	theme	surfactant	1993:2002	arg1	laurate					2011:2017	the anionic surfactant sodium laurate	1981:2017	the anionic surfactant sodium laurate	1981:2017	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	4	73	theme	SL	685:686	arg1	concentration					688:700	the SL concentration	681:700	the SL concentration from 5% to 15%	681:715	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	1	74	from	dispersion	281:290	arg1	matrices					311:318	polymer matrices	303:318	polymer matrices	303:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	5	75	theme	-SLx	827:830	arg1	%					851:851	3 wt%	847:851	3 wt%	847:851	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	5	75	theme	-SLx	827:830	arg1	nanoparticles					832:844	TiO2 -SLx nanoparticles	822:844	TiO2 -SLx nanoparticles (3 wt%)	822:852	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	11	76	theme	TiO2	2147:2150	arg1	nanoparticles					2152:2164	Modified TiO2 nanoparticles	2138:2164	Modified TiO2 nanoparticles	2138:2164	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	77	theme	g·cm-1	1593:1598	arg1	WVP					1575:1577	the lowest WVP	1564:1577	the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 )	1564:1611	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	77	theme	g·cm-1	1593:1598	arg1	·Pa-1					1605:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	78	theme	·s-1	1600:1603	arg1	WVP					1575:1577	the lowest WVP	1564:1577	the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 )	1564:1611	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	78	theme	·s-1	1600:1603	arg1	·Pa-1					1605:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1	1580:1609	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	10	79	theme	ultrasonic-microwave	2023:2042	arg1	technology					2053:2062	ultrasonic-microwave assisted technology	2023:2062	ultrasonic-microwave assisted technology	2023:2062	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	3	80	theme	TiO2	592:595	arg1	dispersibility					574:587	dispersibility	574:587	dispersibility	574:587	The hydrophobicity and dispersibility of TiO2 -SLx increased significantly compared to unmodified nano-TiO2 .
30325500	3	80	theme	TiO2	592:595	arg1	hydrophobicity					555:568	hydrophobicity	555:568	hydrophobicity	555:568	The hydrophobicity and dispersibility of TiO2 -SLx increased significantly compared to unmodified nano-TiO2 .
30325500	5	81	theme	distarch	893:900	arg1	matrices					939:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices	882:946	TiO2 -SLx nanoparticles (3 wt%) were then incorporated into acetylated distarch phosphate/chitosan (ADPS/CS) blended matrices to reinforce the biopolymers.
30325500	8	82	dep	displayed	1554:1562	arg1	decreased					1644:1652	decreased	1644:1652	decreased	1644:1652	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	4	83	theme	15	713:714	arg1	%					708:708	%	708:708	%	708:708	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	1	84	mod	modified	172:179	arg3	SL					217:218	SL	217:218	SL	217:218	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	84	mod	modified	172:179	arg1	TiO2					161:164	TiO2	161:164	TiO2	161:164	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	84	mod	modified	172:179	arg1	dioxide					152:158	Nano-titanium dioxide	138:158	Nano-titanium dioxide (TiO2 )	138:166	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	1	84	mod	modified	172:179	arg3	laurate					208:214	the surfactant sodium laurate	186:214	the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices	186:318	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	3	85	theme	unmodified	638:647	arg1	nano-TiO2					649:657	unmodified nano-TiO2	638:657	unmodified nano-TiO2	638:657	The hydrophobicity and dispersibility of TiO2 -SLx increased significantly compared to unmodified nano-TiO2 .
30325500	9	86	theme	microwave-assisted	1785:1802	arg1	technology					1804:1813	ultrasonic microwave-assisted technology	1774:1813	ultrasonic microwave-assisted technology	1774:1813	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	10	87	theme	anionic	1985:1991	arg1	laurate					2011:2017	the anionic surfactant sodium laurate	1981:2017	the anionic surfactant sodium laurate	1981:2017	PRACTICAL APPLICATION: Nano-titanium dioxide (TiO2 ) nanoparticles were modified using the anionic surfactant sodium laurate via ultrasonic-microwave assisted technology, to improve the dispersion of the TiO2 nanoparticles in polymer matrices.
30325500	3	88	dep	hydrophobicity	555:568	arg1	The					551:553	The	551:553	The	551:553	The hydrophobicity and dispersibility of TiO2 -SLx increased significantly compared to unmodified nano-TiO2 .
30325500	6	89	theme	TiO2	1008:1011	arg1	-SLx					1013:1016	TiO2 -SLx	1008:1016	TiO2 -SLx	1008:1016	Relative to unmodified TiO2 , TiO2 -SLx exhibited a better dispersion capability.
30325500	0	90	theme	Laurate	115:121	arg1	Concentration					123:135	Sodium Laurate Concentration	108:135	Sodium Laurate Concentration	108:135	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	4	91	theme	particles	749:757	arg1	agglomeration					722:734	the agglomeration	718:734	the agglomeration of TiO2 -SLx particles	718:757	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	8	92	theme	-SL15	1543:1547	arg1	film					1549:1552	the ADPS/CS-TiO2 -SL15 film	1526:1552	the ADPS/CS-TiO2 -SL15 film	1526:1552	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	2	93	theme	SL-modified	521:531	arg1	TiO2					533:536	SL-modified TiO2	521:536	SL-modified TiO2 (TiO2 -SLx)	521:548	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	2	93	theme	SL-modified	521:531	arg1	-SLx					544:547	TiO2 -SLx	539:547	TiO2 -SLx	539:547	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	11	94	theme	film	2376:2379	arg1	permeability					2346:2357	water vapor permeability	2334:2357	water vapor permeability	2334:2357	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	11	94	theme	film	2376:2379	arg1	solubility					2319:2328	the water solubility	2309:2328	the water solubility	2309:2328	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	9	95	theme	nanofillers	1852:1862	arg1	preparation					1837:1847	the preparation	1833:1847	the preparation of nanofillers for biopolymer reinforcement	1833:1891	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	2	96	theme	electron	409:416	arg1	microscopy					418:427	scanning electron microscopy	400:427	scanning electron microscopy	400:427	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	0	97	theme	Distarch	11:18	arg1	Films					39:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films	0:43	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	9	98	theme	biopolymer	1868:1877	arg1	reinforcement					1879:1891	biopolymer reinforcement	1868:1891	biopolymer reinforcement	1868:1891	Therefore, SL modification and the use of ultrasonic microwave-assisted technology are promising for the preparation of nanofillers for biopolymer reinforcement.
30325500	2	99	dep	Fourier	336:342	arg1	transform					344:352	transform	344:352	transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses	344:436	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	11	100	theme	tensile	2254:2260	arg1	strength					2262:2269	the tensile strength	2250:2269	the tensile strength of the composite film	2250:2291	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	101	theme	ADPS/CS	1443:1449	arg1	film					1451:1454	the pure ADPS/CS film	1434:1454	the pure ADPS/CS film	1434:1454	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	4	102	theme	TiO2	739:742	arg1	particles					749:757	TiO2 -SLx particles	739:757	TiO2 -SLx particles	739:757	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	6	103	theme	better	1030:1035	arg1	capability					1048:1057	a better dispersion capability	1028:1057	a better dispersion capability	1028:1057	Relative to unmodified TiO2 , TiO2 -SLx exhibited a better dispersion capability.
30325500	8	104	theme	15	1326:1327	arg1	%					1328:1328	%	1328:1328	%	1328:1328	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	105	contain	containing	1284:1293	arg1	film					1279:1282	The composite film	1265:1282	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film)	1265:1357	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	105	contain	containing	1284:1293	arg2	TiO2					1295:1298	TiO2 -SL15	1295:1304	TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film)	1295:1357	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	0	106	theme	Laurate-Modified	68:83	arg1	Nano-TiO2					85:93	Sodium Laurate-Modified Nano-TiO2	61:93	Sodium Laurate-Modified Nano-TiO2	61:93	Acetylated Distarch Phosphate/Chitosan Films Reinforced with Sodium Laurate-Modified Nano-TiO2 : Effects of Sodium Laurate Concentration.
30325500	2	107	theme	TiO2	469:472	arg1	surface					474:480	the TiO2 surface	465:480	the TiO2 surface	465:480	As revealed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses, SL was well adsorbed onto the TiO2 surface through chemical bonding, resulting in SL-modified TiO2 (TiO2 -SLx).
30325500	11	108	theme	film	2288:2291	arg1	strength					2262:2269	the tensile strength	2250:2269	the tensile strength of the composite film	2250:2291	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	11	109	theme	phosphate/Chitosan	2210:2227	arg1	films					2235:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	8	110	theme	-SL25	1482:1486	arg1	film					1488:1491	the ADPS/CS-TiO2 -SL25 film	1465:1491	the ADPS/CS-TiO2 -SL25 film	1465:1491	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	8	111	theme	ADPS/CS-TiO2	1334:1345	arg1	film					1353:1356	ADPS/CS-TiO2 -SL15 film	1334:1356	TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film	1307:1356	The composite film containing TiO2 -SL15 (TiO2 modified with 15% SL; ADPS/CS-TiO2 -SL15 film) displayed the highest TS (31.50 MPa), which was 33.70% higher than that of the pure ADPS/CS film, whereas the ADPS/CS-TiO2 -SL25 film exhibited the lowest E. Further, the ADPS/CS-TiO2 -SL15 film displayed the lowest WVP (0.90 × 10-12 g·cm-1 ·s-1 ·Pa-1 ) and solubility (22.91%), which decreased by 30.23% and 26.03% compared to that of the pure ADPS/CS film, respectively.
30325500	4	112	from	%	715:715	arg1	increase					669:676	an increase	666:676	an increase in the SL concentration from 5% to 15%	666:715	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	4	112	from	%	715:715	arg1	concentration					688:700	the SL concentration	681:700	the SL concentration from 5% to 15%	681:715	With an increase in the SL concentration from 5% to 15%, the agglomeration of TiO2 -SLx particles decreased considerably, while the particles were more uniform.
30325500	1	113	theme	microwave-assisted	236:253	arg1	technology					255:264	ultrasonic microwave-assisted technology	225:264	ultrasonic microwave-assisted technology	225:264	Nano-titanium dioxide (TiO2 ) was modified with the surfactant sodium laurate (SL) via ultrasonic microwave-assisted technology to improve the dispersion of TiO2 in polymer matrices.
30325500	11	114	theme	water	2334:2338	arg1	permeability					2346:2357	water vapor permeability	2334:2357	water vapor permeability	2334:2357	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	7	115	theme	water	1203:1207	arg1	WVP					1229:1231	WVP	1229:1231	WVP	1229:1231	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	7	115	theme	water	1203:1207	arg1	permeability					1215:1226	water vapor permeability	1203:1226	water vapor permeability (WVP)	1203:1232	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	11	116	theme	acetylated	2189:2198	arg1	films					2235:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	acetylated di-starch phosphate/Chitosan blend films	2189:2239	Modified TiO2 nanoparticles were incorporated into acetylated di-starch phosphate/Chitosan blend films, causing the tensile strength of the composite film to increase and the water solubility and water vapor permeability of the composite film to decrease, making the films suitable for packaging applications.
30325500	7	117	theme	films	1151:1155	arg1	TS					1130:1131	TS	1130:1131	TS	1130:1131	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
30325500	7	117	theme	films	1151:1155	arg1	strength					1120:1127	the tensile strength	1108:1127	the tensile strength (TS) of the composite films	1108:1155	Furthermore, as the SL concentration increased, the tensile strength (TS) of the composite films increased, while the elongation at break (E), water vapor permeability (WVP), and solubility all decreased.
29984380	0	0	theme	Interpolyelectrolyte	91:110	arg1	Formation					120:128	Interpolyelectrolyte Complex Formation	91:128	Interpolyelectrolyte Complex Formation	91:128	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	0	theme	Interpolyelectrolyte	91:110	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	1	1	theme	ibuprofen	635:643	arg1	release					624:630	the controlled release	609:630	the controlled release of ibuprofen	609:643	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	2	theme	cystocarpic	260:270	arg1	stage					272:276	cystocarpic stage	260:276	cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina	260:338	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	4	3	theme	skottsbergii	1375:1386	arg1	IPEC					1364:1367	the IPEC	1360:1367	the IPEC of G. skottsbergii	1360:1386	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	2	4	theme	structural	664:673	arg1	determination					675:687	The structural determination	660:687	The structural determination	660:687	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	1	5	theme	present	175:181	arg1	study					183:187	the present study	171:187	the present study	171:187	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	4	6	theme	G.	1372:1373	arg1	skottsbergii					1375:1386	G. skottsbergii	1372:1386	G. skottsbergii	1372:1386	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	7	theme	Sarcothalia	459:469	arg1	crispata					471:478	Sarcothalia crispata	459:478	Sarcothalia crispata	459:478	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	8	theme	model	648:652	arg1	drug					654:657	model drug	648:657	model drug	648:657	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	2	9	theme	ν-carrageenan	896:908	arg1	ν-carrageenan					896:908	ν-carrageenan	896:908	ν-carrageenan	896:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	9	theme	ν-carrageenan	896:908	arg1	ι-					889:890	ι-	889:890	ι-	889:890	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	9	theme	ν-carrageenan	896:908	arg1	amounts					878:884	significant amounts	866:884	significant amounts of ι- and ν-carrageenan	866:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	0	10	theme	Complex	112:118	arg1	Formation					120:128	Interpolyelectrolyte Complex Formation	91:128	Interpolyelectrolyte Complex Formation	91:128	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	10	theme	Complex	112:118	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	1	11	theme	interpolyelectrolytic	356:376	arg1	complexes					378:386	interpolyelectrolytic complexes	356:386	interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata	356:478	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	11	theme	interpolyelectrolytic	356:376	arg1	IPECs					389:393	IPECs	389:393	IPECs	389:393	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	3	12	theme	different	1089:1097	arg1	arrangement					1099:1109	a different arrangement	1087:1109	a different arrangement in the resulting IPEC structure	1087:1141	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	1	13	theme	crispata	471:478	arg1	stage					450:454	cystocarpic stage	438:454	cystocarpic stage of Sarcothalia crispata	438:478	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	0	14	theme	Drug	134:137	arg1	Release					150:156	Drug Controlled Release	134:156	Drug Controlled Release	134:156	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	15	from	skottsbergii	53:64	arg1	Formation					120:128	Interpolyelectrolyte Complex Formation	91:128	Interpolyelectrolyte Complex Formation	91:128	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	15	from	skottsbergii	53:64	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	15	from	skottsbergii	53:64	arg1	Analysis					78:85	Structural Analysis	67:85	Structural Analysis	67:85	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	3	16	theme	IPEC	1128:1131	arg1	structure					1133:1141	the resulting IPEC structure	1114:1141	the resulting IPEC structure	1114:1141	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	3	17	theme	polysaccharide	1033:1046	arg1	chain					1048:1052	the polysaccharide chain	1029:1052	the polysaccharide chain of both carrageenans	1029:1073	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	1	18	theme	study	183:187	arg1	aims					163:166	The aims	159:166	The aims of the present study	159:187	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	19	theme	Gigartina	484:492	arg1	thalli					507:512	Gigartina skottsbergii thalli	484:512	Gigartina skottsbergii thalli	484:512	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	4	20	theme	electron	1183:1190	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy	1174:1201	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	21	theme	skottsbergii	494:505	arg1	thalli					507:512	Gigartina skottsbergii thalli	484:512	Gigartina skottsbergii thalli	484:512	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	4	22	theme	scanning	1174:1181	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy	1174:1201	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	23	theme	crispata	284:291	arg1	stage					272:276	cystocarpic stage	260:276	cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina	260:338	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	3	24	theme	resulting	1118:1126	arg1	structure					1133:1141	the resulting IPEC structure	1114:1141	the resulting IPEC structure	1114:1141	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	4	25	from	kinks	1247:1251	arg1	backbone					1275:1282	the polysaccharide backbone	1256:1282	the polysaccharide backbone	1256:1282	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	4	26	located	observed	1162:1169	arg1	IPEC					1210:1213	the IPEC	1206:1213	the IPEC of S. crispata	1206:1228	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	4	26	located	observed	1162:1169	arg2	pores					1156:1160	The smaller pores	1144:1160	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata	1144:1228	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	3	27	theme	carrageenans	1062:1073	arg1	chain					1048:1052	the polysaccharide chain	1029:1052	the polysaccharide chain of both carrageenans	1029:1073	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	2	28	located	detected	920:927	arg2	ν-carrageenan					896:908	ν-carrageenan	896:908	ν-carrageenan	896:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	28	located	detected	920:927	arg1	polygalactans					937:949	both polygalactans	932:949	both polygalactans	932:949	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	28	located	detected	920:927	arg2	ι-					889:890	ι-	889:890	ι-	889:890	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	28	located	detected	920:927	arg2	amounts					878:884	significant amounts	866:884	significant amounts of ι- and ν-carrageenan	866:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	3	29	theme	diad	971:974	arg1	composition					976:986	diad composition	971:986	diad composition	971:986	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	0	30	theme	Controlled	139:148	arg1	Release					150:156	Drug Controlled Release	134:156	Drug Controlled Release	134:156	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	4	31	theme	smaller	1148:1154	arg1	pores					1156:1160	The smaller pores	1144:1160	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata	1144:1228	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	32	theme	first	218:222	arg1	time					224:227	the first time	214:227	the first time	214:227	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	4	33	theme	ibuprofen	1330:1338	arg1	release					1340:1346	a slower ibuprofen release	1321:1346	a slower ibuprofen release compared to the IPEC of G. skottsbergii	1321:1386	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	4	34	theme	slower	1323:1328	arg1	release					1340:1346	a slower ibuprofen release	1321:1346	a slower ibuprofen release compared to the IPEC of G. skottsbergii	1321:1386	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	3	35	from	differences	956:966	arg1	distribution					1010:1021	their distribution	1004:1021	their distribution along the polysaccharide chain of both carrageenans	1004:1073	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	3	35	from	differences	956:966	arg1	composition					976:986	diad composition	971:986	diad composition	971:986	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	1	36	theme	basic	519:523	arg1	copolymer					548:556	basic butylated methacrylate copolymer	519:556	basic butylated methacrylate copolymer (Eudragit E)	519:569	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	36	theme	basic	519:523	arg1	E					568:568	Eudragit E	559:568	Eudragit E	559:568	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	3	37	from	arrangement	1099:1109	arg1	structure					1133:1141	the resulting IPEC structure	1114:1141	the resulting IPEC structure	1114:1141	The differences in diad composition and possibly in their distribution along the polysaccharide chain of both carrageenans would favor a different arrangement in the resulting IPEC structure.
29984380	4	38	theme	polysaccharide	1260:1273	arg1	backbone					1275:1282	the polysaccharide backbone	1256:1282	the polysaccharide backbone	1256:1282	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	39	theme	butylated	525:533	arg1	copolymer					548:556	basic butylated methacrylate copolymer	519:556	basic butylated methacrylate copolymer (Eudragit E)	519:569	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	39	theme	butylated	525:533	arg1	E					568:568	Eudragit E	559:568	Eudragit E	559:568	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	2	40	theme	G.	840:841	arg1	skottsbergii					843:854	G. skottsbergii	840:854	G. skottsbergii	840:854	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	1	41	theme	methacrylate	535:546	arg1	copolymer					548:556	basic butylated methacrylate copolymer	519:556	basic butylated methacrylate copolymer (Eudragit E)	519:569	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	41	theme	methacrylate	535:546	arg1	E					568:568	Eudragit E	559:568	Eudragit E	559:568	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	2	42	theme	ι-	889:890	arg1	ν-carrageenan					896:908	ν-carrageenan	896:908	ν-carrageenan	896:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	42	theme	ι-	889:890	arg1	ι-					889:890	ι-	889:890	ι-	889:890	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	42	theme	ι-	889:890	arg1	amounts					878:884	significant amounts	866:884	significant amounts of ι- and ν-carrageenan	866:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	4	43	theme	crispata	1221:1228	arg1	IPEC					1210:1213	the IPEC	1206:1213	the IPEC of S. crispata	1206:1228	The smaller pores observed by scanning electron microscopy in the IPEC of S. crispata suggest that the kinks in the polysaccharide backbone are evenly distributed, resulting in a slower ibuprofen release compared to the IPEC of G. skottsbergii.
29984380	1	44	theme	cystocarpic	438:448	arg1	stage					450:454	cystocarpic stage	438:454	cystocarpic stage of Sarcothalia crispata	438:478	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	45	theme	controlled	613:622	arg1	release					624:630	the controlled release	609:630	the controlled release of ibuprofen	609:643	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	2	46	theme	skottsbergii	843:854	arg1	case					832:835	the case	828:835	the case of G. skottsbergii	828:854	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	0	47	theme	Structural	67:76	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	47	theme	Structural	67:76	arg1	Analysis					78:85	Structural Analysis	67:85	Structural Analysis	67:85	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	48	from	crispata	30:37	arg1	Formation					120:128	Interpolyelectrolyte Complex Formation	91:128	Interpolyelectrolyte Complex Formation	91:128	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	48	from	crispata	30:37	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	48	from	crispata	30:37	arg1	Analysis					78:85	Structural Analysis	67:85	Structural Analysis	67:85	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	49	dep	Carrageenans	0:11	arg1	Formation					120:128	Interpolyelectrolyte Complex Formation	91:128	Interpolyelectrolyte Complex Formation	91:128	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	49	dep	Carrageenans	0:11	arg1	Carrageenans					0:11	Carrageenans	0:11	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.	0:157	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	0	49	dep	Carrageenans	0:11	arg1	Analysis					78:85	Structural Analysis	67:85	Structural Analysis	67:85	Carrageenans from Sarcothalia crispata and Gigartina skottsbergii: Structural Analysis and Interpolyelectrolyte Complex Formation for Drug Controlled Release.
29984380	1	50	theme	Eudragit	559:566	arg1	copolymer					548:556	basic butylated methacrylate copolymer	519:556	basic butylated methacrylate copolymer (Eudragit E)	519:569	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	1	50	theme	Eudragit	559:566	arg1	E					568:568	Eudragit E	559:568	Eudragit E	559:568	The aims of the present study were to characterize for the first time the carrageenan extracted from cystocarpic stage of S. crispata collected in the Patagonian coast of Argentina, and to prepare interpolyelectrolytic complexes (IPECs) between the polysaccharide extracted from cystocarpic stage of Sarcothalia crispata and Gigartina skottsbergii thalli, and basic butylated methacrylate copolymer (Eudragit E), in order to test their potential for the controlled release of ibuprofen as model drug.
29984380	2	51	theme	significant	866:876	arg1	ν-carrageenan					896:908	ν-carrageenan	896:908	ν-carrageenan	896:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	51	theme	significant	866:876	arg1	ι-					889:890	ι-	889:890	ι-	889:890	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
29984380	2	51	theme	significant	866:876	arg1	amounts					878:884	significant amounts	866:884	significant amounts of ι- and ν-carrageenan	866:908	The structural determination revealed that the polysaccharides extracted from S. crispata and G. skottsbergii were mainly constituted by κ-carrageenan, particularly in the case of G. skottsbergii; however, significant amounts of ι- and ν-carrageenan were also detected in both polygalactans.
30940581	0	0	theme	chitosan	81:88	arg1	derivatives					90:100	chitosan derivatives	81:100	chitosan derivatives	81:100	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	1	1	theme	enhanced	335:342	arg1	ultrafiltration					344:358	size enhanced ultrafiltration	330:358	size enhanced ultrafiltration (SEUF)	330:365	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	1	1	theme	enhanced	335:342	arg1	SEUF					361:364	SEUF	361:364	SEUF	361:364	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	2	2	from	C2H5	413:416	arg1	TEAC					431:434	TEAC	431:434	TEAC	431:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	7	3	theme	heavy	1206:1210	arg1	rejection					1218:1226	heavy metal rejection	1206:1226	heavy metal rejection	1206:1226	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	8	4	theme	regression	1351:1360	arg1	models					1362:1367	the developed regression models	1337:1367	the developed regression models	1337:1367	The analysis of variance (ANOVA) was performed to validate the developed regression models.
30940581	6	5	theme	initial	1109:1115	arg1	concentration					1117:1129	initial concentration	1109:1129	initial concentration of the feed solution	1109:1150	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	6	dep	ratio	1092:1096	arg1	to					1075:1076	to	1075:1076	to	1075:1076	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	8	7	theme	variance	1294:1301	arg1	analysis					1282:1289	The analysis	1278:1289	The analysis of variance (ANOVA)	1278:1309	The analysis of variance (ANOVA) was performed to validate the developed regression models.
30940581	1	8	dep	removal	290:296	arg1	the					286:288	the	286:288	the	286:288	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	2	9	theme	chromate	541:548	arg1	removal					530:536	the efficient removal	516:536	the efficient removal of chromate	516:548	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	7	10	theme	metal	1212:1216	arg1	rejection					1218:1226	heavy metal rejection	1206:1226	heavy metal rejection	1206:1226	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	6	11	theme	feed	1052:1055	arg1	solution					1057:1064	the feed solution	1048:1064	the feed solution	1048:1064	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	5	12	theme	composite	848:856	arg1	CCD					866:868	CCD	866:868	CCD	866:868	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	5	12	theme	composite	848:856	arg1	design					858:863	the central composite design	836:863	the central composite design (CCD) of response surface methodology (RSM)	836:907	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	6	13	theme	polymer	1067:1073	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	13	theme	polymer	1067:1073	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	4	14	used	used	715:718	arg2	FTIR					690:693	FTIR	690:693	FTIR	690:693	FTIR, SEM, and EDAX were used to characterize the chitosan derivatives before and after the removal of metals.
30940581	4	14	used	used	715:718	arg2	EDAX					705:708	EDAX	705:708	EDAX	705:708	FTIR, SEM, and EDAX were used to characterize the chitosan derivatives before and after the removal of metals.
30940581	4	14	used	used	715:718	arg2	SEM					696:698	SEM	696:698	SEM	696:698	FTIR, SEM, and EDAX were used to characterize the chitosan derivatives before and after the removal of metals.
30940581	6	15	theme	feed	1138:1141	arg1	solution					1143:1150	the feed solution	1134:1150	the feed solution	1134:1150	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	7	16	theme	derivatives	1265:1275	arg1	rejection					1218:1226	heavy metal rejection	1206:1226	heavy metal rejection	1206:1226	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	7	16	theme	derivatives	1265:1275	arg1	capacity					1240:1247	binding capacity	1232:1247	binding capacity	1232:1247	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	2	17	theme	positive	379:386	arg1	C2H5					413:416	The strong positive quaternary ammonium [-N+(C2H5)3]	368:419	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	2	18	theme	ammonium	399:406	arg1	C2H5					413:416	The strong positive quaternary ammonium [-N+(C2H5)3]	368:419	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	2	19	theme	quaternary	388:397	arg1	C2H5					413:416	The strong positive quaternary ammonium [-N+(C2H5)3]	368:419	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	0	20	with	optimization	106:117	arg1	modeling					141:148	response surface modeling	124:148	response surface modeling	124:148	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	6	21	theme	initial	1034:1040	arg1	pH					1042:1043	initial pH	1034:1043	initial pH of the feed solution	1034:1064	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	3	22	with	interactions	632:643	arg1	metals					656:661	heavy metals	650:661	heavy metals such as Cu(II) and Ni(II)	650:687	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	3	22	with	interactions	632:643	arg1	Cu					671:672	Cu	671:672	Cu(II)	671:676	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	3	22	with	interactions	632:643	arg1	Ni					682:683	Ni	682:683	Ni(II)	682:687	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	1	23	theme	carboxymethyl	194:206	arg1	CMCh					218:221	CMCh	218:221	CMCh	218:221	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	1	23	theme	carboxymethyl	194:206	arg1	chitosan					208:215	carboxymethyl chitosan	194:215	carboxymethyl chitosan (CMCh)	194:222	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	4	24	theme	chitosan	740:747	arg1	derivatives					749:759	the chitosan derivatives	736:759	the chitosan derivatives before and after the removal of metals	736:798	FTIR, SEM, and EDAX were used to characterize the chitosan derivatives before and after the removal of metals.
30940581	7	25	theme	chitosan	1256:1263	arg1	derivatives					1265:1275	the chitosan derivatives	1252:1275	the chitosan derivatives	1252:1275	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	2	26	theme	strong	372:377	arg1	C2H5					413:416	The strong positive quaternary ammonium [-N+(C2H5)3]	368:419	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	0	27	theme	surface	133:139	arg1	modeling					141:148	response surface modeling	124:148	response surface modeling	124:148	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	0	28	with	derivatives	90:100	arg1	modeling					141:148	response surface modeling	124:148	response surface modeling	124:148	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	5	29	theme	central	840:846	arg1	CCD					866:868	CCD	866:868	CCD	866:868	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	5	29	theme	central	840:846	arg1	design					858:863	the central composite design	836:863	the central composite design (CCD) of response surface methodology (RSM)	836:907	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	6	30	theme	removal	924:930	arg1	experiments					932:942	The metal ion removal experiments	910:942	The metal ion removal experiments	910:942	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	0	31	theme	response	124:131	arg1	modeling					141:148	response surface modeling	124:148	response surface modeling	124:148	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	3	32	theme	NH2	601:603	arg1	moieties					605:612	NH2 moieties	601:612	NH2 moieties	601:612	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	1	33	dep	chitosan	174:181	arg1	N‑N‑N‑triethylammonium					151:172	N‑N‑N‑triethylammonium	151:172	N‑N‑N‑triethylammonium	151:172	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	6	34	theme	ion	920:922	arg1	experiments					932:942	The metal ion removal experiments	910:942	The metal ion removal experiments	910:942	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	0	35	theme	metals	30:35	arg1	recovery					12:19	recovery	12:19	recovery	12:19	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	0	35	theme	metals	30:35	arg1	Removal					0:6	Removal	0:6	Removal	0:6	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	2	36	theme	chromate	484:491	arg1	anion					493:497	a strong chromate anion	475:497	a strong chromate anion	475:497	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	1	37	used	utilized	273:280	arg2	derivatives					256:266	the two water-soluble chitosan derivatives	225:266	the two water-soluble chitosan derivatives	225:266	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	6	38	theme	metal	914:918	arg1	experiments					932:942	The metal ion removal experiments	910:942	The metal ion removal experiments	910:942	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	0	39	theme	heavy	24:28	arg1	metals					30:35	heavy metals	24:35	heavy metals	24:35	Removal and recovery of heavy metals through size enhanced ultrafiltration using chitosan derivatives and optimization with response surface modeling.
30940581	2	40	theme	strong	477:482	arg1	anion					493:497	a strong chromate anion	475:497	a strong chromate anion	475:497	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	7	41	theme	binding	1232:1238	arg1	capacity					1240:1247	binding capacity	1232:1247	binding capacity	1232:1247	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	2	42	theme	[-N+	408:411	arg1	C2H5					413:416	The strong positive quaternary ammonium [-N+(C2H5)3]	368:419	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	1	43	theme	heavy	314:318	arg1	metals					320:325	heavy metals	314:325	heavy metals	314:325	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	6	44	theme	loading	1084:1090	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	44	theme	loading	1084:1090	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	45	theme	optimum	1006:1012	arg1	conditions					1022:1031	the optimum process conditions	1002:1031	the optimum process conditions	1002:1031	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	3	46	theme	heavy	650:654	arg1	metals					656:661	heavy metals	650:661	heavy metals such as Cu(II) and Ni(II)	650:687	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	3	46	theme	heavy	650:654	arg1	Cu					671:672	Cu	671:672	Cu(II)	671:676	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	3	46	theme	heavy	650:654	arg1	Ni					682:683	Ni	682:683	Ni(II)	682:687	CMCh consists of COOH and NH2 moieties, which facilitate interactions with heavy metals such as Cu(II) and Ni(II).
30940581	1	47	theme	metals	320:325	arg1	recovery					302:309	recovery	302:309	recovery	302:309	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	1	47	theme	metals	320:325	arg1	removal					290:296	removal	290:296	removal	290:296	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	2	48	theme	efficient	520:528	arg1	removal					530:536	the efficient removal	516:536	the efficient removal of chromate	516:548	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	6	49	dep	conducted	949:957	arg1	concentration					1117:1129	initial concentration	1109:1129	initial concentration of the feed solution	1109:1150	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	49	dep	conducted	949:957	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	49	dep	conducted	949:957	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	49	dep	conducted	949:957	arg1	pH					1042:1043	initial pH	1034:1043	initial pH of the feed solution	1034:1064	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	2	50	dep	C2H5	413:416	arg1	cation					421:426	cation	421:426	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC	368:434	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	2	51	dep	Cr	451:452	arg1	VI					454:455	VI	454:455	VI	454:455	The strong positive quaternary ammonium [-N+(C2H5)3] cation in TEAC interacts with Cr(VI), which exists as a strong chromate anion thereby enabling the efficient removal of chromate through ultrafiltration.
30940581	6	52	theme	metal	1078:1082	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	52	theme	metal	1078:1082	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	53	theme	solution	1057:1064	arg1	concentration					1117:1129	initial concentration	1109:1129	initial concentration of the feed solution	1109:1150	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	53	theme	solution	1057:1064	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	53	theme	solution	1057:1064	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	53	theme	solution	1057:1064	arg1	pH					1042:1043	initial pH	1034:1043	initial pH of the feed solution	1034:1064	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	54	theme	solution	1143:1150	arg1	concentration					1117:1129	initial concentration	1109:1129	initial concentration of the feed solution	1109:1150	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	54	theme	solution	1143:1150	arg1	P/M					1099:1101	P/M	1099:1101	P/M	1099:1101	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	54	theme	solution	1143:1150	arg1	ratio					1092:1096	polymer to metal loading ratio	1067:1096	polymer to metal loading ratio (P/M)	1067:1102	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	54	theme	solution	1143:1150	arg1	pH					1042:1043	initial pH	1034:1043	initial pH of the feed solution	1034:1064	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	6	55	theme	process	1014:1020	arg1	conditions					1022:1031	the optimum process conditions	1002:1031	the optimum process conditions	1002:1031	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	4	56	theme	metals	793:798	arg1	removal					782:788	the removal	778:788	the removal of metals	778:798	FTIR, SEM, and EDAX were used to characterize the chitosan derivatives before and after the removal of metals.
30940581	5	57	theme	response	874:881	arg1	RSM					904:906	RSM	904:906	RSM	904:906	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	5	57	theme	response	874:881	arg1	methodology					891:901	response surface methodology	874:901	response surface methodology (RSM)	874:907	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	8	58	theme	developed	1341:1349	arg1	models					1362:1367	the developed regression models	1337:1367	the developed regression models	1337:1367	The analysis of variance (ANOVA) was performed to validate the developed regression models.
30940581	7	59	theme	optimization	1157:1168	arg1	study					1170:1174	The optimization study	1153:1174	The optimization study	1153:1174	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	5	60	theme	surface	883:889	arg1	RSM					904:906	RSM	904:906	RSM	904:906	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	5	60	theme	surface	883:889	arg1	methodology					891:901	response surface methodology	874:901	response surface methodology (RSM)	874:907	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	7	61	dep	rejection	1218:1226	arg1	the					1202:1204	the	1202:1204	the	1202:1204	The optimization study was conducted to maximize the heavy metal rejection and binding capacity of the chitosan derivatives.
30940581	1	62	theme	water-soluble	233:245	arg1	derivatives					256:266	the two water-soluble chitosan derivatives	225:266	the two water-soluble chitosan derivatives	225:266	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	1	63	theme	size	330:333	arg1	ultrafiltration					344:358	size enhanced ultrafiltration	330:358	size enhanced ultrafiltration (SEUF)	330:365	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	1	63	theme	size	330:333	arg1	SEUF					361:364	SEUF	361:364	SEUF	361:364	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
30940581	5	64	theme	methodology	891:901	arg1	CCD					866:868	CCD	866:868	CCD	866:868	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	5	64	theme	methodology	891:901	arg1	design					858:863	the central composite design	836:863	the central composite design (CCD) of response surface methodology (RSM)	836:907	The experiments were designed with the central composite design (CCD) of response surface methodology (RSM).
30940581	6	65	theme	statistical	970:980	arg1	design					982:987	the statistical design	966:987	the statistical design	966:987	The metal ion removal experiments were conducted as per the statistical design to determine the optimum process conditions; initial pH of the feed solution, polymer to metal loading ratio (P/M), and initial concentration of the feed solution.
30940581	1	66	theme	chitosan	247:254	arg1	derivatives					256:266	the two water-soluble chitosan derivatives	225:266	the two water-soluble chitosan derivatives	225:266	N‑N‑N‑triethylammonium chitosan (TEAC) and carboxymethyl chitosan (CMCh), the two water-soluble chitosan derivatives were utilized for the removal and recovery of heavy metals by size enhanced ultrafiltration (SEUF).
31265327	6	0	theme	=	1411:1411	arg1	P					1409:1409	P = 0.006	1409:1417	P = 0.006	1409:1417	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	3	1	contain	containing	795:804	arg1	n					767:767	n = 8	767:771	n = 8	767:771	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	1	contain	containing	795:804	arg2	meals					812:816	mixed meals	806:816	mixed meals	806:816	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	1	contain	containing	795:804	arg1	sorghum					758:764	sorghum	758:764	sorghum (n = 8)	758:772	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	8	2	theme	identical	1826:1834	arg1	calorie					1836:1842	identical calorie	1826:1842	identical calorie	1826:1842	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	3	dep	than	1777:1780	arg1	with					1782:1785	with	1782:1785	with	1782:1785	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	1	4	theme	mixed	187:191	arg1	meal					193:196	a mixed meal	185:196	a mixed meal	185:196	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	2	5	theme	insulin	567:573	arg1	action					575:580	insulin action	567:580	insulin action	567:580	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	2	6	contain	containing	676:685	arg1	carbohydrate					663:674	a simple carbohydrate	654:674	a simple carbohydrate containing mixed meal	654:696	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	2	6	contain	containing	676:685	arg2	meal					693:696	mixed meal	687:696	mixed meal	687:696	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	3	7	theme	=	750:750	arg1	rice					742:745	rice	742:745	rice (n = 8)	742:753	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	7	theme	=	750:750	arg1	n					748:748	n = 8	748:752	n = 8	748:752	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	8	theme	mixed	806:810	arg1	meals					812:816	mixed meals	806:816	mixed meals	806:816	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	8	9	theme	healthy	1878:1884	arg1	adults					1886:1891	healthy adults	1878:1891	healthy adults	1878:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	5	10	theme	endogenous	1040:1049	arg1	production					1059:1068	endogenous glucose production	1040:1068	endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1)	1040:1112	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	10	theme	endogenous	1040:1049	arg1	h-1					1109:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	11	contain	containing	1244:1253	arg2	meals					1255:1259	meals	1255:1259	meals	1255:1259	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	11	contain	containing	1244:1253	arg1	carbohydrate					1231:1242	complex and simple carbohydrate	1212:1242	complex and simple carbohydrate containing meals, respectively	1212:1273	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	2	12	theme	function	593:600	arg1	indices					556:562	indices	556:562	indices of insulin action and β-cell function	556:600	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	2	12	theme	function	593:600	arg1	turnover					543:550	postprandial complex carbohydrate turnover	509:550	postprandial complex carbohydrate turnover	509:550	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	7	13	theme	mixed	1685:1689	arg1	meals					1691:1695	mixed meals	1685:1695	mixed meals	1685:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	5	14	dep	differed	1184:1191	arg1	P					1194:1194	P < 0.01	1194:1201	P < 0.01	1194:1201	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	8	15	from	responsivity	1751:1762	arg1	meals					1817:1821	mixed meals	1811:1821	mixed meals of identical calorie	1811:1842	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	15	from	responsivity	1751:1762	arg1	compositions					1862:1873	macronutrient compositions	1848:1873	macronutrient compositions in healthy adults	1848:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	16	theme	insulin	1720:1726	arg1	sensitivity					1728:1738	higher insulin sensitivity	1713:1738	higher insulin sensitivity	1713:1738	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	7	17	theme	carbohydrate	1661:1672	arg1	turnover					1641:1648	postprandial turnover	1628:1648	postprandial turnover of complex carbohydrate containing mixed meals	1628:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	0	18	theme	carbohydrate	49:60	arg1	metabolism					62:71	complex carbohydrate metabolism	41:71	complex carbohydrate metabolism	41:71	A novel natural tracer method to measure complex carbohydrate metabolism.
31265327	3	19	theme	=	789:789	arg1	n					787:787	n = 16	787:792	n = 16	787:792	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	19	theme	=	789:789	arg1	glucose					778:784	glucose	778:784	glucose (n = 16)	778:793	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	6	20	theme	insulin	1337:1343	arg1	sensitivity					1345:1355	insulin sensitivity	1337:1355	insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006)	1337:1418	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	6	20	theme	insulin	1337:1343	arg1	/pM					1404:1406	32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM	1358:1406	32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM	1358:1406	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	1	21	theme	complex	345:351	arg1	carbohydrates					353:365	complex carbohydrates	345:365	complex carbohydrates	345:365	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	1	22	theme	carbohydrate	157:168	arg1	turnover					170:177	carbohydrate turnover	157:177	carbohydrate turnover	157:177	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	7	23	theme	postprandial	1628:1639	arg1	turnover					1641:1648	postprandial turnover	1628:1648	postprandial turnover of complex carbohydrate containing mixed meals	1628:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	5	24	theme	production	1059:1068	arg1	Rates					962:966	Rates	962:966	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1)	962:1182	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	8	25	theme	complex	1769:1775	arg1	carbohydrates					1794:1806	complex than with simple carbohydrates	1769:1806	complex than with simple carbohydrates	1769:1806	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	2	26	theme	complex	522:528	arg1	turnover					543:550	postprandial complex carbohydrate turnover	509:550	postprandial complex carbohydrate turnover	509:550	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	6	27	theme	simple	1547:1552	arg1	meals					1567:1571	simple carbohydrate meals	1547:1571	simple carbohydrate meals	1547:1571	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	3	28	theme	tracer	842:847	arg1	technique					849:857	the triple tracer technique	831:857	the triple tracer technique to calculate carbohydrate turnover	831:892	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	7	29	contain	containing	1674:1683	arg2	meals					1691:1695	mixed meals	1685:1695	mixed meals	1685:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	7	29	contain	containing	1674:1683	arg1	carbohydrate					1661:1672	complex carbohydrate	1653:1672	complex carbohydrate containing mixed meals	1653:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	2	30	theme	natural	389:395	arg1	enrichment					397:406	the natural enrichment	385:406	the natural enrichment of [13C]polysaccharide in commercially available grains	385:462	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	1	31	theme	triple	84:89	arg1	method					115:120	the triple tracer isotope dilution method	80:120	the triple tracer isotope dilution method	80:120	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	7	32	theme	novel	1587:1591	arg1	approach					1607:1614	a novel triple tracer approach	1585:1614	a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals	1585:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	2	33	theme	available	447:455	arg1	grains					457:462	commercially available grains	434:462	commercially available grains	434:462	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	8	34	from	meals	1817:1821	arg1	adults					1886:1891	healthy adults	1878:1891	healthy adults	1878:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	2	35	theme	mixed	687:691	arg1	meal					693:696	mixed meal	687:696	mixed meal	687:696	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	0	36	theme	natural	8:14	arg1	method					23:28	A novel natural tracer method	0:28	A novel natural tracer method	0:28	A novel natural tracer method to measure complex carbohydrate metabolism.
31265327	1	37	theme	translational	276:288	arg1	applicability					290:302	its translational applicability	272:302	its translational applicability to everyday meals that typically contain complex carbohydrates	272:365	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	1	38	theme	isotope	98:104	arg1	method					115:120	the triple tracer isotope dilution method	80:120	the triple tracer isotope dilution method	80:120	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	3	39	theme	carbohydrate	872:883	arg1	turnover					885:892	carbohydrate turnover	872:892	carbohydrate turnover	872:892	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	2	40	theme	novel	478:482	arg1	method					491:496	a novel tracer method	476:496	a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function	476:600	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	5	41	theme	appearance	984:993	arg1	Rates					962:966	Rates	962:966	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1)	962:1182	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	42	theme	glucose	1118:1124	arg1	disappearance					1126:1138	glucose disappearance	1118:1138	glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1)	1118:1182	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	42	theme	glucose	1118:1124	arg1	h-1					1179:1181	1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1	1141:1181	1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1	1141:1181	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	6	43	from	responsivity	1431:1442	arg1	indices					1326:1332	indices	1326:1332	indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006)	1326:1418	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	1	44	contain	contain	337:343	arg2	carbohydrates					353:365	complex carbohydrates	345:365	complex carbohydrates	345:365	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	1	44	contain	contain	337:343	arg1	meals					316:320	everyday meals	307:320	everyday meals that typically contain complex carbohydrates	307:365	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	6	45	dep	/pM	1404:1406	arg1	P					1409:1409	P = 0.006	1409:1417	P = 0.006	1409:1417	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	1	46	theme	carbohydrate	217:228	arg1	glucose					230:236	the simple carbohydrate glucose	206:236	the simple carbohydrate glucose	206:236	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	5	47	theme	meal	971:974	arg1	appearance					984:993	meal glucose appearance	971:993	meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1)	971:1037	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	47	theme	meal	971:974	arg1	h-1					1034:1036	2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1	996:1036	2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1	996:1036	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	8	48	from	compositions	1862:1873	arg1	adults					1886:1891	healthy adults	1878:1891	healthy adults	1878:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	49	with	responsivity	1751:1762	arg1	carbohydrates					1794:1806	complex than with simple carbohydrates	1769:1806	complex than with simple carbohydrates	1769:1806	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	50	theme	mixed	1811:1815	arg1	meals					1817:1821	mixed meals	1811:1821	mixed meals of identical calorie	1811:1842	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	6	51	dep	index	1457:1461	arg1	/pM					1513:1515	1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM	1464:1515	1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM	1464:1515	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	6	51	dep	index	1457:1461	arg1	<					1520:1520	P < 0.005	1518:1526	P < 0.005	1518:1526	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	3	52	theme	healthy	710:716	arg1	volunteers					718:727	healthy volunteers	710:727	healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals	710:816	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	1	53	theme	carbohydrate	245:256	arg1	source					258:263	the carbohydrate source	241:263	the carbohydrate source	241:263	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	8	54	theme	calorie	1836:1842	arg1	meals					1817:1821	mixed meals	1811:1821	mixed meals of identical calorie	1811:1842	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	54	theme	calorie	1836:1842	arg1	compositions					1862:1873	macronutrient compositions	1848:1873	macronutrient compositions in healthy adults	1848:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	7	55	theme	complex	1653:1659	arg1	carbohydrate					1661:1672	complex carbohydrate	1653:1672	complex carbohydrate containing mixed meals	1653:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	1	56	theme	turnover	170:177	arg1	estimation					143:152	accurate estimation	134:152	accurate estimation of carbohydrate turnover	134:177	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	4	57	theme	macronutrient	935:947	arg1	composition					949:959	macronutrient composition	935:959	macronutrient composition	935:959	All meals were matched for calories and macronutrient composition.
31265327	8	58	theme	macronutrient	1848:1860	arg1	compositions					1862:1873	macronutrient compositions	1848:1873	macronutrient compositions in healthy adults	1848:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	5	59	theme	μM·kg-1·2	1099:1107	arg1	production					1059:1068	endogenous glucose production	1040:1068	endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1)	1040:1112	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	59	theme	μM·kg-1·2	1099:1107	arg1	h-1					1109:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	6	60	theme	significant	1302:1312	arg1	increase					1314:1321	significant increase	1302:1321	significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006)	1302:1418	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	8	61	from	sensitivity	1728:1738	arg1	meals					1817:1821	mixed meals	1811:1821	mixed meals of identical calorie	1811:1842	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	8	61	from	sensitivity	1728:1738	arg1	compositions					1862:1873	macronutrient compositions	1848:1873	macronutrient compositions in healthy adults	1848:1891	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	6	62	theme	disposition	1445:1455	arg1	responsivity					1431:1442	β-cell responsivity	1424:1442	β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005)	1424:1527	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	6	62	theme	disposition	1445:1455	arg1	index					1457:1461	disposition index	1445:1461	disposition index	1445:1461	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	3	63	theme	=	769:769	arg1	n					767:767	n = 8	767:771	n = 8	767:771	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	3	63	theme	=	769:769	arg1	sorghum					758:764	sorghum	758:764	sorghum (n = 8)	758:772	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	5	64	theme	glucose	1051:1057	arg1	production					1059:1068	endogenous glucose production	1040:1068	endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1)	1040:1112	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	64	theme	glucose	1051:1057	arg1	h-1					1109:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	1	65	theme	dilution	106:113	arg1	method					115:120	the triple tracer isotope dilution method	80:120	the triple tracer isotope dilution method	80:120	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	2	66	theme	β-cell	586:591	arg1	function					593:600	β-cell function	586:600	β-cell function	586:600	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	5	67	theme	simple	1224:1229	arg1	carbohydrate					1231:1242	complex and simple carbohydrate	1212:1242	complex and simple carbohydrate containing meals, respectively	1212:1273	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	6	68	theme	P	1518:1518	arg1	<					1520:1520	P < 0.005	1518:1526	P < 0.005	1518:1526	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	2	69	theme	action	575:580	arg1	indices					556:562	indices	556:562	indices of insulin action and β-cell function	556:600	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	2	69	theme	action	575:580	arg1	turnover					543:550	postprandial complex carbohydrate turnover	509:550	postprandial complex carbohydrate turnover	509:550	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	8	70	theme	higher	1713:1718	arg1	sensitivity					1728:1738	higher insulin sensitivity	1713:1738	higher insulin sensitivity	1713:1738	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	1	71	theme	accurate	134:141	arg1	estimation					143:152	accurate estimation	134:152	accurate estimation of carbohydrate turnover	134:177	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	6	72	theme	sensitivity	1345:1355	arg1	indices					1326:1332	indices	1326:1332	indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006)	1326:1418	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	5	73	theme	323	1095:1097	arg1	production					1059:1068	endogenous glucose production	1040:1068	endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1)	1040:1112	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	73	theme	323	1095:1097	arg1	h-1					1109:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1	1071:1111	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	6	74	theme	dl·kg-1·min-2	1499:1511	arg1	/pM					1513:1515	1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM	1464:1515	1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM	1464:1515	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	3	75	theme	triple	835:840	arg1	technique					849:857	the triple tracer technique	831:857	the triple tracer technique to calculate carbohydrate turnover	831:892	We studied healthy volunteers after either rice (n = 8) or sorghum (n = 8) and glucose (n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover.
31265327	2	76	theme	tracer	484:489	arg1	method					491:496	a novel tracer method	476:496	a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function	476:600	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	5	77	theme	complex	1212:1218	arg1	carbohydrate					1231:1242	complex and simple carbohydrate	1212:1242	complex and simple carbohydrate containing meals, respectively	1212:1273	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	8	78	theme	β-cell	1744:1749	arg1	responsivity					1751:1762	β-cell responsivity	1744:1762	β-cell responsivity	1744:1762	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	2	79	theme	carbohydrate	530:541	arg1	turnover					543:550	postprandial complex carbohydrate turnover	509:550	postprandial complex carbohydrate turnover	509:550	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	2	80	theme	simple	656:661	arg1	carbohydrate					663:674	a simple carbohydrate	654:674	a simple carbohydrate containing mixed meal	654:696	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	6	81	from	increase	1314:1321	arg1	indices					1326:1332	indices	1326:1332	indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006)	1326:1418	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	6	82	theme	carbohydrate	1554:1565	arg1	meals					1567:1571	simple carbohydrate meals	1547:1571	simple carbohydrate meals	1547:1571	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	2	83	theme	postprandial	509:520	arg1	turnover					543:550	postprandial complex carbohydrate turnover	509:550	postprandial complex carbohydrate turnover	509:550	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	1	84	theme	simple	210:215	arg1	glucose					230:236	the simple carbohydrate glucose	206:236	the simple carbohydrate glucose	206:236	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	0	85	theme	novel	2:6	arg1	method					23:28	A novel natural tracer method	0:28	A novel natural tracer method	0:28	A novel natural tracer method to measure complex carbohydrate metabolism.
31265327	6	86	dep	32.5 ± 3.5	1358:1367	arg1	dl·kg-1·min-2					1390:1402	dl·kg-1·min-2	1390:1402	dl·kg-1·min-2	1390:1402	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	0	87	theme	tracer	16:21	arg1	method					23:28	A novel natural tracer method	0:28	A novel natural tracer method	0:28	A novel natural tracer method to measure complex carbohydrate metabolism.
31265327	8	88	theme	simple	1787:1792	arg1	carbohydrates					1794:1806	complex than with simple carbohydrates	1769:1806	complex than with simple carbohydrates	1769:1806	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	1	89	theme	tracer	91:96	arg1	method					115:120	the triple tracer isotope dilution method	80:120	the triple tracer isotope dilution method	80:120	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	7	90	theme	triple	1593:1598	arg1	approach					1607:1614	a novel triple tracer approach	1585:1614	a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals	1585:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	2	91	theme	polysaccharide	416:429	arg1	enrichment					397:406	the natural enrichment	385:406	the natural enrichment of [13C]polysaccharide in commercially available grains	385:462	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	5	92	theme	glucose	976:982	arg1	appearance					984:993	meal glucose appearance	971:993	meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1)	971:1037	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	5	92	theme	glucose	976:982	arg1	h-1					1034:1036	2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1	996:1036	2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1	996:1036	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	8	93	with	sensitivity	1728:1738	arg1	carbohydrates					1794:1806	complex than with simple carbohydrates	1769:1806	complex than with simple carbohydrates	1769:1806	We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults.
31265327	5	94	theme	<	1196:1196	arg1	P					1194:1194	P < 0.01	1194:1201	P < 0.01	1194:1201	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	0	95	theme	complex	41:47	arg1	metabolism					62:71	complex carbohydrate metabolism	41:71	complex carbohydrate metabolism	41:71	A novel natural tracer method to measure complex carbohydrate metabolism.
31265327	1	96	theme	everyday	307:314	arg1	meals					316:320	everyday meals	307:320	everyday meals that typically contain complex carbohydrates	307:365	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
31265327	5	97	theme	disappearance	1126:1138	arg1	Rates					962:966	Rates	962:966	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1)	962:1182	Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg-1·2 h-1), endogenous glucose production (-835 ± 283 vs. -1,123 ± 323 μM·kg-1·2 h-1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg-1·2 h-1) differed (P < 0.01) between complex and simple carbohydrate containing meals, respectively.
31265327	2	98	from	enrichment	397:406	arg1	grains					457:462	commercially available grains	434:462	commercially available grains	434:462	Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal.
31265327	6	99	theme	β-cell	1424:1429	arg1	responsivity					1431:1442	β-cell responsivity	1424:1442	β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005)	1424:1527	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	6	99	theme	β-cell	1424:1429	arg1	index					1457:1461	disposition index	1445:1461	disposition index	1445:1461	Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10-5 (dl·kg-1·min-2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10-14 (dl·kg-1·min-2)/pM, P < 0.005) with complex than simple carbohydrate meals.
31265327	7	100	theme	tracer	1600:1605	arg1	approach					1607:1614	a novel triple tracer approach	1585:1614	a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals	1585:1695	We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals.
31265327	1	101	theme	glucose	230:236	arg1	use					199:201	use	199:201	use of the simple carbohydrate glucose as the carbohydrate source	199:263	While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates.
30253830	6	0	dep	separated	1255:1263	arg1	baseline					1246:1253	baseline	1246:1253	baseline	1246:1253	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	7	1	theme	time	1494:1497	arg1	RSDs					1472:1475	intraday RSDs	1463:1475	intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1463:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	6	2	theme	peak	1275:1278	arg1	shape					1280:1284	good peak shape	1270:1284	good peak shape without any buffer salt in mobile phase	1270:1324	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	4	3	theme	retention	848:856	arg1	mechanism					858:866	a mixed-mode retention mechanism	835:866	a mixed-mode retention mechanism of the as-synthesized stationary phase	835:905	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	7	4	theme	excellent	1418:1426	arg1	repeatability					1444:1456	excellent chromatographic repeatability	1418:1456	excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1418:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	3	5	theme	3.25 nm-thick	686:698	arg1	layer					708:712	the 3.25 nm-thick polymer layer	682:712	the 3.25 nm-thick polymer layer fabricated on the pore inner surface	682:749	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	1	6	theme	modified	195:202	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	6	theme	modified	195:202	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	5	7	theme	liquid	1164:1169	arg1	chromatography					1171:1184	reversed-phase liquid chromatography	1149:1184	reversed-phase liquid chromatography (RPLC) mode	1149:1196	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	7	theme	liquid	1164:1169	arg1	RPLC					1187:1190	RPLC	1187:1190	RPLC	1187:1190	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	7	8	theme	packing	1389:1395	arg1	material					1397:1404	The packing material	1385:1404	The packing material	1385:1404	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	2	9	theme	glycidyl	345:352	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	9	theme	glycidyl	345:352	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	4	10	theme	phase	901:905	arg1	mechanism					858:866	a mixed-mode retention mechanism	835:866	a mixed-mode retention mechanism of the as-synthesized stationary phase	835:905	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	0	11	theme	performance	129:139	arg1	chromatography					148:161	high performance liquid chromatography	124:161	high performance liquid chromatography stationary phase	124:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	2	12	theme	cross-linked	327:338	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	12	theme	cross-linked	327:338	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	7	13	theme	great	1590:1594	arg1	stability					1599:1607	great pH stability	1590:1607	great pH stability	1590:1607	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	0	14	theme	chromatography	148:161	arg1	phase					174:178	high performance liquid chromatography stationary phase	124:178	high performance liquid chromatography stationary phase	124:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	5	15	theme	interaction	1067:1077	arg1	HILIC					1102:1106	HILIC	1102:1106	HILIC	1102:1106	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	15	theme	interaction	1067:1077	arg1	chromatography					1086:1099	hydrophilic interaction liquid chromatography	1055:1099	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	3	16	theme	porous	586:591	arg1	matrix					667:672	the matrix	663:672	the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability	663:783	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	3	16	theme	porous	586:591	arg1	silica					603:608	The porous spherical silica	582:608	The porous spherical silica (4 μm diameter) with 300 Å pore size	582:645	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	3	16	theme	porous	586:591	arg1	diameter					616:623	4 μm diameter	611:623	4 μm diameter	611:623	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	2	17	theme	"	570:570	arg1	reaction					572:579	a [3 + 2] "click" reaction	554:579	a [3 + 2] "click" reaction	554:579	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	3	18	theme	pore	732:735	arg1	surface					743:749	the pore inner surface	728:749	the pore inner surface	728:749	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	6	19	theme	buffer	1298:1303	arg1	salt					1305:1308	any buffer salt	1294:1308	any buffer salt in mobile phase	1294:1324	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	8	20	theme	extract	1700:1706	arg1	separation					1678:1687	the separation	1674:1687	the separation of ginseng extract	1674:1706	Finally, the stationary phase was applied to the separation of ginseng extract.
30253830	4	21	theme	as-synthesized	875:888	arg1	phase					901:905	the as-synthesized stationary phase	871:905	the as-synthesized stationary phase	871:905	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	7	22	from	10.2	1622:1625	arg1	n = 7					1579:1583	n = 7	1579:1583	n = 7	1579:1583	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	7	22	from	10.2	1622:1625	arg1	%					1576:1576	less than 0.33%	1562:1576	less than 0.33% (n = 7)	1562:1584	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	7	22	from	10.2	1622:1625	arg1	stability					1599:1607	great pH stability	1590:1607	great pH stability	1590:1607	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	6	23	theme	silanol	1369:1375	arg1	groups					1377:1382	the silanol groups	1365:1382	the silanol groups	1365:1382	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	0	24	theme	cross-linked	81:92	arg1	methacrylate					108:119	cross-linked poly glycidyl methacrylate	81:119	cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase	81:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	1	25	theme	new	183:185	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	25	theme	new	183:185	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	7	26	theme	interday	1548:1555	arg1	RSDs					1557:1560	interday RSDs	1548:1560	interday RSDs	1548:1560	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	2	27	theme	polymer	536:542	arg1	layer					544:548	the polymer layer	532:548	the polymer layer	532:548	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	0	28	theme	stationary	163:172	arg1	phase					174:178	high performance liquid chromatography stationary phase	124:178	high performance liquid chromatography stationary phase	124:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	6	29	theme	effective	1342:1350	arg1	shielding					1352:1360	the effective shielding	1338:1360	the effective shielding of the silanol groups	1338:1382	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	5	30	theme	chromatography	1086:1099	arg1	mode					1109:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	31	theme	beads	463:467	arg1	surface					415:421	the outer surface	405:421	the outer surface as well as pore inner surface of silica beads	405:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	31	theme	beads	463:467	arg1	surface					445:451	pore inner surface	434:451	the outer surface as well as pore inner surface of silica beads	405:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	32	theme	outer	409:413	arg1	surface					415:421	the outer surface	405:421	the outer surface as well as pore inner surface of silica beads	405:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	1	33	theme	stationary	305:314	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	33	theme	stationary	305:314	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	2	34	theme	inner	439:443	arg1	surface					445:451	pore inner surface	434:451	the outer surface as well as pore inner surface of silica beads	405:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	3	35	theme	300 Å	631:635	arg1	size					642:645	300 Å pore size	631:645	300 Å pore size	631:645	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	5	36	from	plates/m	1124:1131	arg1	mode					1109:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	0	37	theme	maltose	30:36	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	0	37	theme	maltose	30:36	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	5	38	theme	column	995:1000	arg1	efficiency					1002:1011	column efficiency	995:1011	column efficiency	995:1011	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	39	theme	in-situ	473:479	arg1	polymerization					481:494	in-situ polymerization	473:494	in-situ polymerization	473:494	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	3	40	with	silica	603:608	arg1	size					642:645	300 Å pore size	631:645	300 Å pore size	631:645	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	0	41	theme	polymer-silica	47:60	arg1	composite					62:70	polymer-silica composite	47:70	polymer-silica composite	47:70	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	6	42	from	salt	1305:1308	arg1	phase					1320:1324	mobile phase	1313:1324	mobile phase	1313:1324	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	7	43	theme	nucleosides	1507:1517	arg1	time					1494:1497	the retention time	1480:1497	the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1480:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	2	44	theme	pGMA	368:371	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	44	theme	pGMA	368:371	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	1	45	theme	performance	264:274	arg1	HPLC					299:302	HPLC	299:302	HPLC	299:302	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	45	theme	performance	264:274	arg1	chromatography					283:296	high performance liquid chromatography	259:296	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	7	46	theme	retention	1484:1492	arg1	time					1494:1497	the retention time	1480:1497	the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1480:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	1	47	theme	chromatography	283:296	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	47	theme	chromatography	283:296	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	6	48	theme	good	1270:1273	arg1	shape					1280:1284	good peak shape	1270:1284	good peak shape without any buffer salt in mobile phase	1270:1324	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	7	49	theme	intraday	1463:1470	arg1	RSDs					1472:1475	intraday RSDs	1463:1475	intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1463:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	4	50	theme	mixed-mode	837:846	arg1	mechanism					858:866	a mixed-mode retention mechanism	835:866	a mixed-mode retention mechanism of the as-synthesized stationary phase	835:905	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	7	51	theme	chromatographic	1428:1442	arg1	repeatability					1444:1456	excellent chromatographic repeatability	1418:1456	excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1418:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	3	52	theme	polymer	700:706	arg1	layer					708:712	the 3.25 nm-thick polymer layer	682:712	the 3.25 nm-thick polymer layer fabricated on the pore inner surface	682:749	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	1	53	theme	maltose	187:193	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	53	theme	maltose	187:193	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	4	54	theme	U-shape	799:805	arg1	curves					818:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	5	55	from	plates/m	1029:1036	arg1	mode					1109:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	56	theme	methacrylate	354:365	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	56	theme	methacrylate	354:365	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	8	57	theme	stationary	1642:1651	arg1	phase					1653:1657	the stationary phase	1638:1657	the stationary phase	1638:1657	Finally, the stationary phase was applied to the separation of ginseng extract.
30253830	1	58	theme	polymer-silica	204:217	arg1	composite					219:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite	181:227	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	58	theme	polymer-silica	204:217	arg1	phase					316:320	high performance liquid chromatography (HPLC) stationary phase	259:320	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	7	59	theme	pH	1596:1597	arg1	stability					1599:1607	great pH stability	1590:1607	great pH stability	1590:1607	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	4	60	theme	retention	808:816	arg1	curves					818:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	0	61	theme	liquid	141:146	arg1	chromatography					148:161	high performance liquid chromatography	124:161	high performance liquid chromatography stationary phase	124:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	2	62	theme	poly	340:343	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	62	theme	poly	340:343	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	4	63	theme	stationary	890:899	arg1	phase					901:905	the as-synthesized stationary phase	871:905	the as-synthesized stationary phase	871:905	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	6	64	theme	mobile	1313:1318	arg1	phase					1320:1324	mobile phase	1313:1324	mobile phase	1313:1324	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	5	65	theme	hydrophilic	1055:1065	arg1	HILIC					1102:1106	HILIC	1102:1106	HILIC	1102:1106	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	65	theme	hydrophilic	1055:1065	arg1	chromatography					1086:1099	hydrophilic interaction liquid chromatography	1055:1099	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	7	66	dep	10.2	1622:1625	arg1	to					1619:1620	to	1619:1620	to	1619:1620	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	2	67	link	cross-linked	327:338	arg1	layer					374:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer	323:378	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	2	67	link	cross-linked	327:338	arg1	bonded					395:400	bonded	395:400	bonded	395:400	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	5	68	theme	liquid	1079:1084	arg1	HILIC					1102:1106	HILIC	1102:1106	HILIC	1102:1106	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	68	theme	liquid	1079:1084	arg1	chromatography					1086:1099	hydrophilic interaction liquid chromatography	1055:1099	hydrophilic interaction liquid chromatography (HILIC) mode	1055:1112	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	69	theme	click	565:569	arg1	reaction					572:579	a [3 + 2] "click" reaction	554:579	a [3 + 2] "click" reaction	554:579	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	3	70	theme	inner	737:741	arg1	surface					743:749	the pore inner surface	728:749	the pore inner surface	728:749	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	8	71	theme	ginseng	1692:1698	arg1	extract					1700:1706	ginseng extract	1692:1706	ginseng extract	1692:1706	Finally, the stationary phase was applied to the separation of ginseng extract.
30253830	6	72	theme	groups	1377:1382	arg1	shielding					1352:1360	the effective shielding	1338:1360	the effective shielding of the silanol groups	1338:1382	Nucleotides and their bases were baseline separated with good peak shape without any buffer salt in mobile phase, suggesting the effective shielding of the silanol groups.
30253830	3	73	theme	spherical	593:601	arg1	matrix					667:672	the matrix	663:672	the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability	663:783	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	3	73	theme	spherical	593:601	arg1	silica					603:608	The porous spherical silica	582:608	The porous spherical silica (4 μm diameter) with 300 Å pore size	582:645	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	3	73	theme	spherical	593:601	arg1	diameter					616:623	4 μm diameter	611:623	4 μm diameter	611:623	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	0	74	theme	glycidyl	99:106	arg1	methacrylate					108:119	cross-linked poly glycidyl methacrylate	81:119	cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase	81:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	0	75	theme	poly	94:97	arg1	methacrylate					108:119	cross-linked poly glycidyl methacrylate	81:119	cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase	81:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	0	76	theme	high	124:127	arg1	chromatography					148:161	high performance liquid chromatography	124:161	high performance liquid chromatography stationary phase	124:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	5	77	theme	polar	913:917	arg1	analytes					933:940	Both polar and non-polar analytes	908:940	Both polar and non-polar analytes	908:940	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	78	theme	silica	456:461	arg1	beads					463:467	silica beads	456:467	silica beads	456:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	5	79	theme	chromatography	1171:1184	arg1	mode					1193:1196	reversed-phase liquid chromatography (RPLC) mode	1149:1196	reversed-phase liquid chromatography (RPLC) mode	1149:1196	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	80	theme	non-polar	923:931	arg1	analytes					933:940	Both polar and non-polar analytes	908:940	Both polar and non-polar analytes	908:940	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	2	81	theme	pore	434:437	arg1	surface					445:451	pore inner surface	434:451	the outer surface as well as pore inner surface of silica beads	405:467	The cross-linked poly glycidyl methacrylate (pGMA) layer was chemically bonded to the outer surface as well as pore inner surface of silica beads via in-situ polymerization, and then maltose was modified onto the polymer layer via a [3 + 2] "click" reaction.
30253830	7	82	dep	%	1534:1534	arg1	n = 7					1579:1583	n = 7	1579:1583	n = 7	1579:1583	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	7	82	dep	%	1534:1534	arg1	%					1576:1576	less than 0.33%	1562:1576	less than 0.33% (n = 7)	1562:1584	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	7	82	dep	%	1534:1534	arg1	stability					1599:1607	great pH stability	1590:1607	great pH stability	1590:1607	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	0	83	link	cross-linked	81:92	arg1	methacrylate					108:119	cross-linked poly glycidyl methacrylate	81:119	cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase	81:178	Preparation and evaluation of maltose modified polymer-silica composite based on cross-linked poly glycidyl methacrylate as high performance liquid chromatography stationary phase.
30253830	4	84	theme	typical	790:796	arg1	curves					818:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves	786:823	The typical 'U-shape' retention curves indicated a mixed-mode retention mechanism of the as-synthesized stationary phase.
30253830	7	85	with	repeatability	1444:1456	arg1	RSDs					1472:1475	intraday RSDs	1463:1475	intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1463:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	5	86	theme	reversed-phase	1149:1162	arg1	chromatography					1171:1184	reversed-phase liquid chromatography	1149:1184	reversed-phase liquid chromatography (RPLC) mode	1149:1196	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	86	theme	reversed-phase	1149:1162	arg1	RPLC					1187:1190	RPLC	1187:1190	RPLC	1187:1190	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	7	87	theme	less	1519:1522	arg1	nucleosides					1507:1517	five nucleosides	1502:1517	five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2)	1502:1626	The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five nucleosides less than 0.048% (n = 3) and interday RSDs less than 0.33% (n = 7) and great pH stability (from 1.5 to 10.2).
30253830	3	88	theme	pore	637:640	arg1	size					642:645	300 Å pore size	631:645	300 Å pore size	631:645	The porous spherical silica (4 μm diameter) with 300 Å pore size was selected as the matrix so that the 3.25 nm-thick polymer layer fabricated on the pore inner surface would not affect its permeability.
30253830	1	89	theme	high	259:262	arg1	HPLC					299:302	HPLC	299:302	HPLC	299:302	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	89	theme	high	259:262	arg1	chromatography					283:296	high performance liquid chromatography	259:296	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	5	90	theme	stationary	973:982	arg1	phase					984:988	the stationary phase	969:988	the stationary phase with column efficiency	969:1011	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	5	91	with	phase	984:988	arg1	efficiency					1002:1011	column efficiency	995:1011	column efficiency	995:1011	Both polar and non-polar analytes could be well separated on the stationary phase with column efficiency reaching 123809 plates/m for guanosine in hydrophilic interaction liquid chromatography (HILIC) mode and 46808 plates/m for fluorene in reversed-phase liquid chromatography (RPLC) mode, respectively.
30253830	1	92	theme	liquid	276:281	arg1	HPLC					299:302	HPLC	299:302	HPLC	299:302	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30253830	1	92	theme	liquid	276:281	arg1	chromatography					283:296	high performance liquid chromatography	259:296	high performance liquid chromatography (HPLC) stationary phase	259:320	A new maltose modified polymer-silica composite was fabricated and applied as high performance liquid chromatography (HPLC) stationary phase.
30414900	0	0	theme	carcinoma	87:95	arg1	cells					103:107	human hepatocellular carcinoma HepG2 cells	66:107	human hepatocellular carcinoma HepG2 cells	66:107	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	4	1	theme	morphology	667:676	arg1	observation					678:688	nuclear morphology observation	659:688	nuclear morphology observation	659:688	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	6	2	theme	fluorescence	885:896	arg1	quantification					898:911	Real-time fluorescence quantification	875:911	Real-time fluorescence quantification	875:911	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	1	3	theme	antitumor	226:234	arg1	activity					236:243	antitumor activity	226:243	antitumor activity	226:243	In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated.
30414900	0	4	theme	hepatocellular	72:85	arg1	carcinoma					87:95	human hepatocellular carcinoma	66:95	human hepatocellular carcinoma HepG2 cells	66:107	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	3	5	theme	Congo	504:508	arg1	experiment					514:523	Congo red experiment	504:523	Congo red experiment	504:523	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	2	6	from	l‑rhamnose	333:342	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	4	7	theme	antitumor	767:775	arg1	effect					777:782	significant antitumor effect	755:782	significant antitumor effect	755:782	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	3	8	contain	have	582:585	arg2	effect					598:603	anti-tumor effect	587:603	anti-tumor effect	587:603	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	3	8	contain	have	582:585	arg1	structure					559:567	a triple helix structure	544:567	a triple helix structure	544:567	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	0	9	theme	HepG2	97:101	arg1	cells					103:107	human hepatocellular carcinoma HepG2 cells	66:107	human hepatocellular carcinoma HepG2 cells	66:107	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	6	10	theme	Western	917:923	arg1	blot					925:928	Western blot	917:928	Western blot	917:928	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	7	11	theme	antitumor	1118:1126	arg1	drug					1128:1131	an antitumor drug	1115:1131	an antitumor drug in foods	1115:1140	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	7	11	theme	antitumor	1118:1126	arg1	GP					1095:1096	GP	1095:1096	GP	1095:1096	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	4	12	theme	significant	755:765	arg1	effect					777:782	significant antitumor effect	755:782	significant antitumor effect	755:782	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	2	13	dep	composition	295:305	arg1	the					291:293	the	291:293	the	291:293	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	4	14	theme	morphology	635:644	arg1	observation					646:656	cell morphology observation	630:656	cell morphology observation	630:656	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	5	15	theme	Flow	785:788	arg1	cytometry					790:798	Flow cytometry	785:798	Flow cytometry	785:798	Flow cytometry suggested that GP could promote apoptosis and arrest cells in G0-G1 phase.
30414900	0	16	theme	Ginger	0:5	arg1	polysaccharides					7:21	Ginger polysaccharides	0:21	Ginger polysaccharides	0:21	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	4	17	theme	cell	630:633	arg1	observation					646:656	cell morphology observation	630:656	cell morphology observation	630:656	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	7	18	used	used	1107:1110	arg2	drug					1128:1131	an antitumor drug	1115:1131	an antitumor drug in foods	1115:1140	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	7	18	used	used	1107:1110	arg2	GP					1095:1096	GP	1095:1096	GP	1095:1096	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	2	19	theme	absorption	469:478	arg1	peak					480:483	the characteristic absorption peak	450:483	the characteristic absorption peak of polysaccharide	450:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	0	20	theme	human	66:70	arg1	carcinoma					87:95	human hepatocellular carcinoma	66:95	human hepatocellular carcinoma HepG2 cells	66:107	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	3	21	theme	helix	553:557	arg1	structure					559:567	a triple helix structure	544:567	a triple helix structure	544:567	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	2	22	theme	3.64:5.37:3.04:61.03:26.91	415:440	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	6	23	theme	Fas	988:990	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	5	24	theme	G0-G1	862:866	arg1	phase					868:872	G0-G1 phase	862:872	G0-G1 phase	862:872	Flow cytometry suggested that GP could promote apoptosis and arrest cells in G0-G1 phase.
30414900	6	25	theme	caspase-3	999:1007	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	0	26	theme	cell	31:34	arg1	arrest					42:47	cell cycle arrest	31:47	cell cycle arrest	31:47	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	4	27	contain	had	751:753	arg1	GP					748:749	GP	748:749	GP	748:749	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	4	27	contain	had	751:753	arg2	effect					777:782	significant antitumor effect	755:782	significant antitumor effect	755:782	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	2	28	from	d‑arabinose	345:355	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	2	29	theme	molar	400:404	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	6	30	theme	Bax	983:985	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	6	31	theme	Real-time	875:883	arg1	quantification					898:911	Real-time fluorescence quantification	875:911	Real-time fluorescence quantification	875:911	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	3	32	theme	triple	546:551	arg1	structure					559:567	a triple helix structure	544:567	a triple helix structure	544:567	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	1	33	theme	enzymatic	180:188	arg1	method					190:195	enzymatic method	180:195	enzymatic method	180:195	In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated.
30414900	2	34	theme	characteristic	454:467	arg1	peak					480:483	the characteristic absorption peak	450:483	the characteristic absorption peak of polysaccharide	450:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	4	35	theme	reactive	694:701	arg1	species					710:716	reactive oxygen species	694:716	reactive oxygen species	694:716	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	0	36	theme	cycle	36:40	arg1	arrest					42:47	cell cycle arrest	31:47	cell cycle arrest	31:47	Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
30414900	4	37	dep	assay	623:627	arg1	observation					718:728	observation	718:728	observation	718:728	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	5	38	from	cells	853:857	arg1	phase					868:872	G0-G1 phase	862:872	G0-G1 phase	862:872	Flow cytometry suggested that GP could promote apoptosis and arrest cells in G0-G1 phase.
30414900	2	39	from	d‑mannose	358:366	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	4	40	theme	oxygen	703:708	arg1	species					710:716	reactive oxygen species	694:716	reactive oxygen species	694:716	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	7	41	from	drug	1128:1131	arg1	foods					1136:1140	foods	1136:1140	foods	1136:1140	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	3	42	contain	had	540:542	arg1	GP					537:538	GP	537:538	GP	537:538	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	3	42	contain	had	540:542	arg2	structure					559:567	a triple helix structure	544:567	a triple helix structure	544:567	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	2	43	theme	GP	325:326	arg1	proportion					311:320	proportion	311:320	proportion	311:320	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	2	43	theme	GP	325:326	arg1	composition					295:305	composition	295:305	composition	295:305	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	2	44	from	d‑glucose	369:377	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	6	45	theme	p53	1018:1020	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	7	46	theme	foods	1183:1187	arg1	development					1157:1167	the development	1153:1167	the development of functional foods	1153:1187	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
30414900	1	47	theme	ginger	125:130	arg1	GP					148:149	GP	148:149	GP	148:149	In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated.
30414900	1	47	theme	ginger	125:130	arg1	polysaccharide					132:145	ginger polysaccharide	125:145	ginger polysaccharide (GP)	125:150	In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated.
30414900	1	48	theme	chemical	202:209	arg1	properties					211:220	its chemical properties	198:220	its chemical properties	198:220	In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated.
30414900	2	49	theme	polysaccharide	488:501	arg1	peak					480:483	the characteristic absorption peak	450:483	the characteristic absorption peak of polysaccharide	450:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	3	50	theme	anti-tumor	587:596	arg1	effect					598:603	anti-tumor effect	587:603	anti-tumor effect	587:603	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	2	51	from	d‑galactose	383:393	arg1	ratio					406:410	a molar ratio	398:410	a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide	398:501	The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04:61.03:26.91, GP had the characteristic absorption peak of polysaccharide.
30414900	3	52	theme	red	510:512	arg1	experiment					514:523	Congo red experiment	504:523	Congo red experiment	504:523	Congo red experiment showed that GP had a triple helix structure, which could have anti-tumor effect.
30414900	6	53	theme	Bcl-2	1059:1063	arg1	expression					1045:1054	the expression	1041:1054	the expression of Bcl-2	1041:1063	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	6	54	theme	p21	1010:1012	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	6	55	theme	FasL	993:996	arg1	expression					969:978	the expression	965:978	the expression of Bax, Fas, FasL, caspase-3, p21 and p53	965:1020	Real-time fluorescence quantification and Western blot revealed that GP could up-regulate the expression of Bax, Fas, FasL, caspase-3, p21 and p53, and down-regulate the expression of Bcl-2.
30414900	4	56	theme	MTT	619:621	arg1	assay					623:627	MTT assay	619:627	MTT assay	619:627	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	4	57	theme	nuclear	659:665	arg1	observation					678:688	nuclear morphology observation	659:688	nuclear morphology observation	659:688	Furthermore, MTT assay, cell morphology observation, nuclear morphology observation and reactive oxygen species observation demonstrated that GP had significant antitumor effect.
30414900	7	58	theme	functional	1172:1181	arg1	foods					1183:1187	functional foods	1172:1187	functional foods	1172:1187	These studies suggested that GP would be used as an antitumor drug in foods to promote the development of functional foods.
31194520	0	0	theme	Cellulose	78:86	arg1	Nanofibrils					88:98	Cellulose Nanofibrils	78:98	Cellulose Nanofibrils	78:98	Accounting for Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils.
31194520	4	1	theme	High-resolution	519:533	arg1	approach					565:572	the best approach	556:572	the best approach to describe the morphological features of nanocelluloses	556:629	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	4	1	theme	High-resolution	519:533	arg1	imaging					535:541	High-resolution imaging	519:541	High-resolution imaging	519:541	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	9	2	theme	atomic	1419:1424	arg1	microscopy					1432:1441	atomic force microscopy	1419:1441	atomic force microscopy	1419:1441	The images obtained by the latter, a faster imaging method, were correlated with those from atomic force microscopy.
31194520	7	3	theme	CNFs	1086:1089	arg1	affinity					1074:1081	the affinity	1070:1081	the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state	1070:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	4	4	theme	nanocelluloses	616:629	arg1	features					604:611	the morphological features	586:611	the morphological features of nanocelluloses	586:629	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	7	5	from	geometry	1164:1171	arg1	state					1200:1204	the dried state	1190:1204	the dried state	1190:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	10	6	theme	CNF	1476:1478	arg1	area					1464:1467	The cross-sectional area	1444:1467	The cross-sectional area of the CNF	1444:1478	The cross-sectional area of the CNF is reconstructed by cross-correlating the widths and heights obtained by the two techniques.
31194520	8	7	theme	new	1278:1280	arg1	microscopy					1243:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	8	7	theme	new	1278:1280	arg1	method					1282:1287	a new method	1276:1287	a new method to assess the dimensions of the CNFs	1276:1324	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	1	8	theme	special	170:176	arg1	attention					178:186	special attention	170:186	special attention	170:186	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	8	theme	special	170:176	arg1	blocks					200:205	building blocks	191:205	building blocks for the development of advanced materials and composites	191:262	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	8	9	theme	Negative-contrast	1207:1223	arg1	microscopy					1243:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	8	9	theme	Negative-contrast	1207:1223	arg1	method					1282:1287	a new method	1276:1287	a new method to assess the dimensions of the CNFs	1276:1324	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	9	10	dep	imaging	1371:1377	arg1	faster					1364:1369	faster	1364:1369	faster	1364:1369	The images obtained by the latter, a faster imaging method, were correlated with those from atomic force microscopy.
31194520	0	11	theme	Nanofibrils	88:98	arg1	Dimensions					64:73	the Dimensions	60:73	the Dimensions of Cellulose Nanofibrils	60:98	Accounting for Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils.
31194520	3	12	dep	solid	503:507	arg1	supports					509:516	supports	509:516	supports	509:516	However, this correlation is often evaluated indirectly by process-centered approaches or by accessing a single dimensionality of CNFs adsorbed on solid supports.
31194520	8	13	theme	electron	1234:1241	arg1	microscopy					1243:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	8	13	theme	electron	1234:1241	arg1	method					1282:1287	a new method	1276:1287	a new method to assess the dimensions of the CNFs	1276:1324	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	1	14	theme	cellulose	126:134	arg1	CNFs					149:152	CNFs	149:152	CNFs	149:152	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	14	theme	cellulose	126:134	arg1	nanofibrils					136:146	Mechanically fibrillated cellulose nanofibrils	101:146	Mechanically fibrillated cellulose nanofibrils (CNFs)	101:153	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	7	15	theme	anisotropic	1136:1146	arg1	geometry					1164:1171	a highly anisotropic cross-sectional geometry	1127:1171	a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state	1127:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	2	16	theme	CNF	294:296	arg1	morphology					298:307	CNF morphology	294:307	CNF morphology	294:307	A correlation exists between CNF morphology and the properties of the materials they form.
31194520	7	17	with	CNFs	1086:1089	arg1	substrate					1100:1108	the substrate	1096:1108	the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state	1096:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	8	18	theme	scanning	1225:1232	arg1	microscopy					1243:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy	1207:1252	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	8	18	theme	scanning	1225:1232	arg1	method					1282:1287	a new method	1276:1287	a new method to assess the dimensions of the CNFs	1276:1324	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	7	19	theme	cross-sectional	1148:1162	arg1	geometry					1164:1171	a highly anisotropic cross-sectional geometry	1127:1171	a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state	1127:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	7	20	theme	substantial	1031:1041	arg1	flattening					1043:1052	a substantial flattening	1029:1052	a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state	1029:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	3	21	theme	single	461:466	arg1	dimensionality					468:481	a single dimensionality	459:481	a single dimensionality of CNFs adsorbed on solid supports	459:516	However, this correlation is often evaluated indirectly by process-centered approaches or by accessing a single dimensionality of CNFs adsorbed on solid supports.
31194520	7	22	dep	geometry	1164:1171	arg1	ellipsoidal					1174:1184	ellipsoidal	1174:1184	ellipsoidal	1174:1184	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	6	23	theme	adsorption	924:933	arg1	effects					935:941	adsorption effects	924:941	adsorption effects	924:941	By considering soft matter imaging and adsorption effects, we provide evidence of the deformation of CNFs upon casting and drying.
31194520	10	24	theme	cross-sectional	1448:1462	arg1	area					1464:1467	The cross-sectional area	1444:1467	The cross-sectional area of the CNF	1444:1478	The cross-sectional area of the CNF is reconstructed by cross-correlating the widths and heights obtained by the two techniques.
31194520	1	25	theme	building	191:198	arg1	attention					178:186	special attention	170:186	special attention	170:186	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	25	theme	building	191:198	arg1	blocks					200:205	building blocks	191:205	building blocks for the development of advanced materials and composites	191:262	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	5	26	with	deformations	714:725	arg1	substrate					795:803	the substrate	791:803	the substrate	791:803	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	9	27	theme	imaging	1371:1377	arg1	method					1379:1384	a faster imaging method	1362:1384	a faster imaging method	1362:1384	The images obtained by the latter, a faster imaging method, were correlated with those from atomic force microscopy.
31194520	9	27	theme	imaging	1371:1377	arg1	latter					1354:1359	latter	1354:1359	latter	1354:1359	The images obtained by the latter, a faster imaging method, were correlated with those from atomic force microscopy.
31194520	5	28	theme	CNFs	734:737	arg1	interactions					773:784	interactions	773:784	interactions with the substrate	773:803	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	5	28	theme	CNFs	734:737	arg1	deformations					714:725	possible deformations	705:725	possible deformations of the CNFs arising from capillary forces	705:767	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	1	29	theme	Mechanically	101:112	arg1	CNFs					149:152	CNFs	149:152	CNFs	149:152	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	29	theme	Mechanically	101:112	arg1	nanofibrils					136:146	Mechanically fibrillated cellulose nanofibrils	101:146	Mechanically fibrillated cellulose nanofibrils (CNFs)	101:153	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	3	30	theme	CNFs	486:489	arg1	dimensionality					468:481	a single dimensionality	459:481	a single dimensionality of CNFs adsorbed on solid supports	459:516	However, this correlation is often evaluated indirectly by process-centered approaches or by accessing a single dimensionality of CNFs adsorbed on solid supports.
31194520	0	31	theme	Substrate	15:23	arg1	Interactions					25:36	Substrate Interactions	15:36	Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils	15:98	Accounting for Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils.
31194520	10	32	dep	widths	1522:1527	arg1	the					1518:1520	the	1518:1520	the	1518:1520	The cross-sectional area of the CNF is reconstructed by cross-correlating the widths and heights obtained by the two techniques.
31194520	1	33	theme	fibrillated	114:124	arg1	CNFs					149:152	CNFs	149:152	CNFs	149:152	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	33	theme	fibrillated	114:124	arg1	nanofibrils					136:146	Mechanically fibrillated cellulose nanofibrils	101:146	Mechanically fibrillated cellulose nanofibrils (CNFs)	101:153	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	7	34	theme	dried	1194:1198	arg1	state					1200:1204	the dried state	1190:1204	the dried state	1190:1204	We determine a substantial flattening associated with the affinity of CNFs with the substrate corresponding to a highly anisotropic cross-sectional geometry (ellipsoidal) in the dried state.
31194520	4	35	theme	morphological	590:602	arg1	features					604:611	the morphological features	586:611	the morphological features of nanocelluloses	586:629	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	5	36	with	interactions	773:784	arg1	substrate					795:803	the substrate	791:803	the substrate	791:803	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	4	37	theme	best	560:563	arg1	approach					565:572	the best approach	556:572	the best approach to describe the morphological features of nanocelluloses	556:629	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	4	37	theme	best	560:563	arg1	imaging					535:541	High-resolution imaging	519:541	High-resolution imaging	519:541	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	5	38	theme	capillary	752:760	arg1	forces					762:767	capillary forces	752:767	capillary forces	752:767	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	6	39	theme	matter	905:910	arg1	imaging					912:918	soft matter imaging	900:918	soft matter imaging	900:918	By considering soft matter imaging and adsorption effects, we provide evidence of the deformation of CNFs upon casting and drying.
31194520	8	40	theme	CNFs	1321:1324	arg1	dimensions					1303:1312	the dimensions	1299:1312	the dimensions of the CNFs	1299:1324	Negative-contrast scanning electron microscopy is also introduced as a new method to assess the dimensions of the CNFs.
31194520	6	41	theme	CNFs	986:989	arg1	deformation					971:981	the deformation	967:981	the deformation of CNFs upon casting and drying	967:1013	By considering soft matter imaging and adsorption effects, we provide evidence of the deformation of CNFs upon casting and drying.
31194520	5	42	theme	possible	705:712	arg1	deformations					714:725	possible deformations	705:725	possible deformations of the CNFs arising from capillary forces	705:767	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	6	43	theme	soft	900:903	arg1	imaging					912:918	soft matter imaging	900:918	soft matter imaging	900:918	By considering soft matter imaging and adsorption effects, we provide evidence of the deformation of CNFs upon casting and drying.
31194520	2	44	dep	properties	317:326	arg1	form					350:353	form	350:353	form	350:353	A correlation exists between CNF morphology and the properties of the materials they form.
31194520	3	45	theme	process-centered	415:430	arg1	approaches					432:441	process-centered approaches	415:441	process-centered approaches	415:441	However, this correlation is often evaluated indirectly by process-centered approaches or by accessing a single dimensionality of CNFs adsorbed on solid supports.
31194520	6	46	theme	deformation	971:981	arg1	evidence					955:962	evidence	955:962	evidence of the deformation of CNFs upon casting and drying	955:1013	By considering soft matter imaging and adsorption effects, we provide evidence of the deformation of CNFs upon casting and drying.
31194520	4	47	theme	adsorption	646:655	arg1	effects					657:663	adsorption effects	646:663	adsorption effects	646:663	High-resolution imaging is currently the best approach to describe the morphological features of nanocelluloses; nevertheless, adsorption effects need to be accounted for.
31194520	2	48	theme	materials	335:343	arg1	morphology					298:307	CNF morphology	294:307	CNF morphology	294:307	A correlation exists between CNF morphology and the properties of the materials they form.
31194520	2	48	theme	materials	335:343	arg1	properties					317:326	the properties	313:326	the properties of the materials they form	313:353	A correlation exists between CNF morphology and the properties of the materials they form.
31194520	1	49	theme	materials	239:247	arg1	development					215:225	the development	211:225	the development of advanced materials and composites	211:262	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	0	50	theme	Dimensions	64:73	arg1	Measurement					45:55	the Measurement	41:55	the Measurement of the Dimensions of Cellulose Nanofibrils	41:98	Accounting for Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils.
31194520	5	51	theme	dimensions	873:882	arg1	determination					834:846	the determination	830:846	the determination of their cross-sectional dimensions	830:882	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	9	52	theme	force	1426:1430	arg1	microscopy					1432:1441	atomic force microscopy	1419:1441	atomic force microscopy	1419:1441	The images obtained by the latter, a faster imaging method, were correlated with those from atomic force microscopy.
31194520	0	53	from	Interactions	25:36	arg1	Measurement					45:55	the Measurement	41:55	the Measurement of the Dimensions of Cellulose Nanofibrils	41:98	Accounting for Substrate Interactions in the Measurement of the Dimensions of Cellulose Nanofibrils.
31194520	5	54	theme	cross-sectional	857:871	arg1	dimensions					873:882	their cross-sectional dimensions	851:882	their cross-sectional dimensions	851:882	For instance, possible deformations of the CNFs arising from capillary forces and interactions with the substrate need to be considered in the determination of their cross-sectional dimensions.
31194520	1	55	theme	advanced	230:237	arg1	materials					239:247	advanced materials	230:247	advanced materials	230:247	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
31194520	1	56	theme	composites	253:262	arg1	development					215:225	the development	211:225	the development of advanced materials and composites	211:262	Mechanically fibrillated cellulose nanofibrils (CNFs) have attracted special attention as building blocks for the development of advanced materials and composites.
30879652	3	0	with	particles	522:530	arg1	size					551:554	mean particle size	537:554	mean particle size of 124.2 ± 2.5 nm	537:572	CNCC seemed to be rod-like particles with mean particle size of 124.2 ± 2.5 nm.
30879652	8	1	theme	%	996:996	arg1	CO-PEP					1009:1014	50% HPMC based CO-PEP	994:1014	50% HPMC based CO-PEP	994:1014	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	5	2	theme	interaction	766:776	arg1	dependent					744:752	dependent	744:752	dependent	744:752	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	5	2	theme	interaction	766:776	arg1	combination					697:707	The combination	693:707	The combination of CNC and CMC-Na in CNCC	693:733	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	4	3	theme	crystal	604:610	arg1	state					612:616	crystal state	604:616	crystal state	604:616	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	0	4	theme	cellulosic	95:104	arg1	derivatives					106:116	cellulosic derivatives	95:116	cellulosic derivatives	95:116	Redispersible Pickering emulsion powder stabilized by nanocrystalline cellulose combining with cellulosic derivatives.
30879652	8	5	theme	drug	1058:1061	arg1	ability					1071:1077	high drug loading ability	1053:1077	high drug loading ability	1053:1077	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	8	6	theme	loading	1063:1069	arg1	ability					1071:1077	high drug loading ability	1053:1077	high drug loading ability	1053:1077	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	8	7	theme	composite	1029:1037	arg1	particle					1039:1046	large composite particle	1023:1046	large composite particle	1023:1046	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	3	8	theme	mean	537:540	arg1	size					551:554	mean particle size	537:554	mean particle size of 124.2 ± 2.5 nm	537:572	CNCC seemed to be rod-like particles with mean particle size of 124.2 ± 2.5 nm.
30879652	3	9	theme	124.2 ± 2.5 nm	559:572	arg1	size					551:554	mean particle size	537:554	mean particle size of 124.2 ± 2.5 nm	537:572	CNCC seemed to be rod-like particles with mean particle size of 124.2 ± 2.5 nm.
30879652	7	10	theme	distinctive	901:911	arg1	layer					921:925	the distinctive barrier layer	897:925	the distinctive barrier layer around oil droplet	897:944	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	9	11	theme	cellulose	1190:1198	arg1	nanocrystals					1200:1211	cellulose nanocrystals	1190:1211	cellulose nanocrystals combining with water-soluble cellulosic derivatives	1190:1263	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	6	12	theme	CNCC	824:827	arg1	size					797:800	The mean particle size	779:800	The mean particle size of optimum CO-PE/0.25%CNCC	779:827	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	5	13	from	combination	697:707	arg1	CNCC					730:733	CNCC	730:733	CNCC	730:733	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	5	14	theme	physical	757:764	arg1	interaction					766:776	physical interaction	757:776	physical interaction	757:776	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	9	15	theme	Novel	1120:1124	arg1	emulsions					1159:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	8	16	theme	superior	1093:1100	arg1	redispersibility					1102:1117	superior redispersibility	1093:1117	superior redispersibility	1093:1117	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	6	17	theme	%	823:823	arg1	CNCC					824:827	optimum CO-PE/0.25%CNCC	805:827	optimum CO-PE/0.25%CNCC	805:827	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	9	18	theme	redispersible	1126:1138	arg1	emulsions					1159:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	2	19	theme	carboxymethyl	414:426	arg1	sodium					438:443	carboxymethyl cellulose sodium	414:443	carboxymethyl cellulose sodium (CNCC)	414:450	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	2	19	theme	carboxymethyl	414:426	arg1	CNCC					446:449	CNCC	446:449	CNCC	446:449	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	0	20	theme	nanocrystalline	54:68	arg1	cellulose					70:78	nanocrystalline cellulose	54:78	nanocrystalline cellulose combining with cellulosic derivatives	54:116	Redispersible Pickering emulsion powder stabilized by nanocrystalline cellulose combining with cellulosic derivatives.
30879652	6	21	theme	CO-PE/0.25	813:822	arg1	%					823:823	%	823:823	%	823:823	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	7	22	theme	particle	866:873	arg1	stabilizer					875:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	0	23	theme	Pickering	14:22	arg1	powder					33:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder stabilized by nanocrystalline cellulose combining with cellulosic derivatives.
30879652	4	24	theme	cellulose	583:591	arg1	structure					593:601	cellulose structure	583:601	cellulose structure	583:601	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	6	25	theme	optimum	805:811	arg1	CNCC					824:827	optimum CO-PE/0.25%CNCC	805:827	optimum CO-PE/0.25%CNCC	805:827	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	9	26	theme	Pickering	1149:1157	arg1	emulsions					1159:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	0	27	theme	Redispersible	0:12	arg1	powder					33:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder stabilized by nanocrystalline cellulose combining with cellulosic derivatives.
30879652	9	28	theme	powdered	1140:1147	arg1	emulsions					1159:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions	1120:1167	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	8	29	theme	high	1053:1056	arg1	ability					1071:1077	high drug loading ability	1053:1077	high drug loading ability	1053:1077	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	7	30	theme	CNCC	855:858	arg1	stabilizer					875:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	7	31	theme	based	860:864	arg1	stabilizer					875:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer	851:884	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	1	32	theme	carboxymethyl	201:213	arg1	CMC-Na					233:238	carboxymethyl cellulose sodium (CMC-Na)	201:239	carboxymethyl cellulose sodium (CMC-Na)	201:239	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	0	33	theme	emulsion	24:31	arg1	powder					33:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder	0:38	Redispersible Pickering emulsion powder stabilized by nanocrystalline cellulose combining with cellulosic derivatives.
30879652	3	34	theme	rod-like	513:520	arg1	particles					522:530	rod-like particles	513:530	rod-like particles with mean particle size of 124.2 ± 2.5 nm	513:572	CNCC seemed to be rod-like particles with mean particle size of 124.2 ± 2.5 nm.
30879652	1	35	theme	cellulose	215:223	arg1	CMC-Na					233:238	carboxymethyl cellulose sodium (CMC-Na)	201:239	carboxymethyl cellulose sodium (CMC-Na)	201:239	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	36	dep	emulsions	350:358	arg1	powder					360:365	powder	360:365	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	36	dep	emulsions	350:358	arg1	CO-PEP					368:373	CO-PEP	368:373	CO-PEP	368:373	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	37	theme	sodium	225:230	arg1	CMC-Na					233:238	carboxymethyl cellulose sodium (CMC-Na)	201:239	carboxymethyl cellulose sodium (CMC-Na)	201:239	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	38	theme	novel	307:311	arg1	emulsions					350:358	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	6	39	theme	particle	788:795	arg1	size					797:800	The mean particle size	779:800	The mean particle size of optimum CO-PE/0.25%CNCC	779:827	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	2	40	theme	cellulose	428:436	arg1	sodium					438:443	carboxymethyl cellulose sodium	414:443	carboxymethyl cellulose sodium (CNCC)	414:450	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	2	40	theme	cellulose	428:436	arg1	CNCC					446:449	CNCC	446:449	CNCC	446:449	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	1	41	theme	redispersible	313:325	arg1	emulsions					350:358	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	6	42	theme	mean	783:786	arg1	size					797:800	The mean particle size	779:800	The mean particle size of optimum CO-PE/0.25%CNCC	779:827	The mean particle size of optimum CO-PE/0.25%CNCC was 0.569 ± 0.023 μm.
30879652	2	43	theme	homogenization	468:481	arg1	technology					483:492	homogenization technology	468:492	homogenization technology	468:492	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	1	44	theme	camellia	327:334	arg1	emulsions					350:358	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	5	45	theme	CNC	712:714	arg1	dependent					744:752	dependent	744:752	dependent	744:752	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	5	45	theme	CNC	712:714	arg1	combination					697:707	The combination	693:707	The combination of CNC and CMC-Na in CNCC	693:733	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	1	46	theme	study	141:145	arg1	objective					123:131	The objective	119:131	The objective of this study	119:145	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	47	theme	oil	336:338	arg1	emulsions					350:358	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	8	48	theme	large	1023:1027	arg1	particle					1039:1046	large composite particle	1023:1046	large composite particle	1023:1046	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	8	49	theme	based	1003:1007	arg1	CO-PEP					1009:1014	50% HPMC based CO-PEP	994:1014	50% HPMC based CO-PEP	994:1014	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	9	50	theme	cellulosic	1242:1251	arg1	derivatives					1253:1263	water-soluble cellulosic derivatives	1228:1263	water-soluble cellulosic derivatives	1228:1263	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	5	51	theme	CMC-Na	720:725	arg1	dependent					744:752	dependent	744:752	dependent	744:752	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	5	51	theme	CMC-Na	720:725	arg1	combination					697:707	The combination	693:707	The combination of CNC and CMC-Na in CNCC	693:733	The combination of CNC and CMC-Na in CNCC might be dependent of physical interaction.
30879652	8	52	theme	redispersibility	951:966	arg1	results					968:974	The redispersibility results	947:974	The redispersibility results	947:974	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	8	53	theme	50	994:995	arg1	%					996:996	%	996:996	%	996:996	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	1	54	theme	cellulose	157:165	arg1	CNC					181:183	CNC	181:183	CNC	181:183	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	54	theme	cellulose	157:165	arg1	nanocrystals					167:178	cellulose nanocrystals	157:178	cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer	157:294	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	4	55	theme	thermal	622:628	arg1	property					630:637	thermal property	622:637	thermal property	622:637	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	9	56	theme	water-soluble	1228:1240	arg1	derivatives					1253:1263	water-soluble cellulosic derivatives	1228:1263	water-soluble cellulosic derivatives	1228:1263	Novel redispersible powdered Pickering emulsions could be prepared by cellulose nanocrystals combining with water-soluble cellulosic derivatives.
30879652	2	57	theme	Cellulose	377:385	arg1	nanocrystals					387:398	Cellulose nanocrystals	377:398	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC)	377:450	Cellulose nanocrystals modified with carboxymethyl cellulose sodium (CNCC) was prepared by homogenization technology.
30879652	7	58	theme	oil	934:936	arg1	droplet					938:944	oil droplet	934:944	oil droplet	934:944	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	4	59	theme	CNCC	642:645	arg1	state					612:616	crystal state	604:616	crystal state	604:616	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	4	59	theme	CNCC	642:645	arg1	property					630:637	thermal property	622:637	thermal property	622:637	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	4	59	theme	CNCC	642:645	arg1	structure					593:601	cellulose structure	583:601	cellulose structure	583:601	And the cellulose structure, crystal state and thermal property of CNCC remained unchanged during the homogenization.
30879652	1	60	theme	Pickering	340:348	arg1	emulsions					350:358	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	novel redispersible camellia oil Pickering emulsions powder (CO-PEP)	307:374	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	8	61	theme	HPMC	998:1001	arg1	CO-PEP					1009:1014	50% HPMC based CO-PEP	994:1014	50% HPMC based CO-PEP	994:1014	The redispersibility results demonstrated that 50% HPMC based CO-PEP formed large composite particle with high drug loading ability and exhibited superior redispersibility.
30879652	3	62	theme	particle	542:549	arg1	size					551:554	mean particle size	537:554	mean particle size of 124.2 ± 2.5 nm	537:572	CNCC seemed to be rod-like particles with mean particle size of 124.2 ± 2.5 nm.
30879652	7	63	theme	barrier	913:919	arg1	layer					921:925	the distinctive barrier layer	897:925	the distinctive barrier layer around oil droplet	897:944	The CNCC based particle stabilizer might form the distinctive barrier layer around oil droplet.
30879652	1	64	theme	hydroxypropyl	245:257	arg1	HPMC					276:279	HPMC	276:279	HPMC	276:279	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
30879652	1	64	theme	hydroxypropyl	245:257	arg1	methylcellulose					259:273	hydroxypropyl methylcellulose	245:273	hydroxypropyl methylcellulose (HPMC)	245:280	The objective of this study is to use cellulose nanocrystals (CNC) combining with carboxymethyl cellulose sodium (CMC-Na) and hydroxypropyl methylcellulose (HPMC) as stabilizer to prepare novel redispersible camellia oil Pickering emulsions powder (CO-PEP).
29230818	2	0	theme	simple	529:534	arg1	approach					576:583	A simple and environmentally friendly analytical approach	527:583	A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector	527:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	6	1	theme	deteriorative	1424:1436	arg1	flora					1438:1442	deteriorative flora	1424:1442	deteriorative flora	1424:1442	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	2	theme	nutritional	1581:1591	arg1	properties					1593:1602	their nutritional properties	1575:1602	their nutritional properties	1575:1602	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	1	3	theme	carbohydrates	185:197	arg1	quantification					158:171	The quantification	154:171	The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	154:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	4	dep	yeasts	914:919	arg1	flora					946:950	main spoilage flora	932:950	main spoilage flora in strawberry	932:964	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	6	5	dep	CONCLUSION	1289:1298	arg1	demonstrate					1342:1352	demonstrate	1342:1352	demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties	1342:1602	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	2	6	theme	mono-	730:734	arg1	determination					697:709	simultaneous determination	684:709	simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices	684:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	1	7	theme	present	199:205	arg1	carbohydrates					185:197	the main carbohydrates	176:197	the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	176:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	8	theme	main	932:935	arg1	flora					946:950	main spoilage flora	932:950	main spoilage flora in strawberry	932:964	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	0	9	theme	non-thermal	119:129	arg1	treatments					131:140	non-thermal treatments	119:140	non-thermal treatments	119:140	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	2	10	theme	present	754:760	arg1	mono-					730:734	mono-	730:734	mono-	730:734	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	11	dep	RESULTS	519:525	arg1	developed					670:678	developed	670:678	was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices	666:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	1	12	from	present	199:205	arg1	juices					221:226	strawberry juices	210:226	strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	210:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	13	theme	spoilage	937:944	arg1	flora					946:950	main spoilage flora	932:950	main spoilage flora in strawberry	932:964	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	1	14	theme	non-thermal	300:310	arg1	ultrasound					337:346	ultrasound	337:346	ultrasound	337:346	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	14	theme	non-thermal	300:310	arg1	vanillin					324:331	vanillin	324:331	vanillin	324:331	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	14	theme	non-thermal	300:310	arg1	techniques					312:321	non-thermal techniques	300:321	non-thermal techniques (vanillin and ultrasound)	300:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	2	15	theme	FOS	722:724	arg1	determination					697:709	simultaneous determination	684:709	simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices	684:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	3	16	theme	yeasts	914:919	arg1	growth					904:909	the growth	900:909	the growth of yeasts and molds (main spoilage flora in strawberry)	900:965	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	16	theme	yeasts	914:919	arg1	consumption					873:883	the consumption	869:883	the consumption of sucrose	869:894	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	2	17	theme	analytical	565:574	arg1	approach					576:583	A simple and environmentally friendly analytical approach	527:583	A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector	527:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	18	theme	inulin	714:719	arg1	determination					697:709	simultaneous determination	684:709	simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices	684:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	1	19	dep	BACKGROUND	143:152	arg1	conducted					353:361	conducted	353:361	conducted	353:361	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	2	20	with	chromatography	618:631	arg1	detector					657:664	a reflection index detector	638:664	a reflection index detector	638:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	21	from	juices	769:774	arg1	present					754:760	present	754:760	present	754:760	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	22	theme	friendly	556:563	arg1	approach					576:583	A simple and environmentally friendly analytical approach	527:583	A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector	527:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	1	23	attach	present	199:205	arg1	juices					221:226	strawberry juices	210:226	strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	210:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	23	attach	present	199:205	arg2	carbohydrates					185:197	the main carbohydrates	176:197	the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	176:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	4	24	theme	sucrose	1040:1046	arg1	consumption					1048:1058	no sucrose consumption	1037:1058	no sucrose consumption	1037:1058	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
29230818	1	25	theme	refrigerated	497:508	arg1	storage					510:516	refrigerated storage	497:516	refrigerated storage	497:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	7	26	theme	Chemical	1623:1630	arg1	Industry					1632:1639	Chemical Industry	1623:1639	Chemical Industry	1623:1639	© 2017 Society of Chemical Industry.
29230818	4	27	theme	sample	1103:1108	arg1	storage					1080:1086	storage	1080:1086	storage of the treated sample	1080:1108	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
29230818	1	28	theme	strawberry	210:219	arg1	juices					221:226	strawberry juices	210:226	strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	210:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	2	29	attach	present	754:760	arg2	mono-					730:734	mono-	730:734	mono-	730:734	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	29	attach	present	754:760	arg1	juices					769:774	the juices	765:774	the juices	765:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	1	30	theme	evolution	403:411	arg1	relationship					442:453	their relationship	436:453	their relationship with fungal deterioration over 14 days of refrigerated storage	436:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	30	theme	evolution	403:411	arg1	investigation					382:394	an investigation	379:394	an investigation of the evolution of these compounds	379:430	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	31	theme	storage	510:516	arg1	14 days					486:492	14 days	486:492	14 days of refrigerated storage	486:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	32	located	observed	971:978	arg1	control					1014:1020	control	1014:1020	control	1014:1020	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	32	located	observed	971:978	arg1	samples					1005:1011	untreated samples	995:1011	untreated samples (control)	995:1021	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	32	located	observed	971:978	arg2	relationship					848:859	a direct relationship	839:859	a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry)	839:965	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	1	33	with	relationship	442:453	arg1	deterioration					467:479	fungal deterioration	460:479	fungal deterioration over 14 days of refrigerated storage	460:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	2	34	theme	simultaneous	684:695	arg1	determination					697:709	simultaneous determination	684:709	simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices	684:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	0	35	dep	juices	96:101	arg1	preserved					103:111	preserved	103:111	juices preserved using non-thermal treatments	96:140	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	3	36	theme	untreated	995:1003	arg1	control					1014:1020	control	1014:1020	control	1014:1020	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	36	theme	untreated	995:1003	arg1	samples					1005:1011	untreated samples	995:1011	untreated samples (control)	995:1021	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	0	37	theme	carbohydrate	21:32	arg1	composition					34:44	carbohydrate composition	21:44	carbohydrate composition	21:44	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	3	38	theme	carbohydrates	809:821	arg1	evolution					796:804	the evolution	792:804	the evolution of carbohydrates	792:821	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	2	39	theme	liquid	611:616	arg1	chromatography					618:631	high-performance liquid chromatography	594:631	high-performance liquid chromatography with a reflection index detector	594:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	40	from	present	754:760	arg1	juices					769:774	the juices	765:774	the juices	765:774	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	4	41	theme	treatments	1164:1173	arg1	efficiency					1134:1143	the efficiency	1130:1143	the efficiency of the non-thermal treatments for controlling yeasts and mold growth	1130:1212	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
29230818	6	42	theme	good	1514:1517	arg1	addition					1478:1485	the addition	1474:1485	the addition of inulin and FOS	1474:1503	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	42	theme	good	1514:1517	arg1	strategy					1519:1526	a good strategy	1512:1526	a good strategy for functionalizing them, as well as improving their nutritional properties	1512:1602	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	0	43	theme	fungal	50:55	arg1	deterioration					57:69	fungal deterioration	50:69	fungal deterioration	50:69	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	2	44	theme	high-performance	594:609	arg1	chromatography					618:631	high-performance liquid chromatography	594:631	high-performance liquid chromatography with a reflection index detector	594:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	6	45	theme	FOS	1501:1503	arg1	addition					1478:1485	the addition	1474:1485	the addition of inulin and FOS	1474:1503	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	45	theme	FOS	1501:1503	arg1	strategy					1519:1526	a good strategy	1512:1526	a good strategy for functionalizing them, as well as improving their nutritional properties	1512:1602	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	1	46	dep	juices	221:226	arg1	enriched					228:235	enriched	228:235	enriched with inulin and fructo-oligosaccharides (FOS)	228:281	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	46	dep	juices	221:226	arg1	preserved					287:295	preserved	287:295	preserved by non-thermal techniques (vanillin and ultrasound)	287:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	4	47	theme	non-thermal	1152:1162	arg1	treatments					1164:1173	the non-thermal treatments	1148:1173	the non-thermal treatments for controlling yeasts and mold growth	1148:1212	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
29230818	6	48	theme	non-thermal	1359:1369	arg1	treatments					1371:1380	non-thermal treatments	1359:1380	non-thermal treatments	1359:1380	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	1	49	theme	compounds	422:430	arg1	evolution					403:411	the evolution	399:411	the evolution of these compounds	399:430	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	2	50	theme	environmentally	540:554	arg1	approach					576:583	A simple and environmentally friendly analytical approach	527:583	A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector	527:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	2	51	theme	index	651:655	arg1	detector					657:664	a reflection index detector	638:664	a reflection index detector	638:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	6	52	theme	flora	1438:1442	arg1	growth					1414:1419	the growth	1410:1419	the growth of deteriorative flora in strawberry juices	1410:1463	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	53	from	growth	1414:1419	arg1	juices					1458:1463	strawberry juices	1447:1463	strawberry juices	1447:1463	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	54	theme	inulin	1490:1495	arg1	addition					1478:1485	the addition	1474:1485	the addition of inulin and FOS	1474:1503	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	54	theme	inulin	1490:1495	arg1	strategy					1519:1526	a good strategy	1512:1526	a good strategy for functionalizing them, as well as improving their nutritional properties	1512:1602	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	6	55	theme	strawberry	1447:1456	arg1	juices					1458:1463	strawberry juices	1447:1463	strawberry juices	1447:1463	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	2	56	theme	reflection	640:649	arg1	detector					657:664	a reflection index detector	638:664	a reflection index detector	638:664	RESULTS A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices.
29230818	3	57	theme	molds	925:929	arg1	growth					904:909	the growth	900:909	the growth of yeasts and molds (main spoilage flora in strawberry)	900:965	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	57	theme	molds	925:929	arg1	consumption					873:883	the consumption	869:883	the consumption of sucrose	869:894	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	58	theme	direct	841:846	arg1	relationship					848:859	a direct relationship	839:859	a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry)	839:965	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	1	59	dep	techniques	312:321	arg1	ultrasound					337:346	ultrasound	337:346	ultrasound	337:346	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	59	dep	techniques	312:321	arg1	vanillin					324:331	vanillin	324:331	vanillin	324:331	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	59	dep	techniques	312:321	arg1	techniques					312:321	non-thermal techniques	300:321	non-thermal techniques (vanillin and ultrasound)	300:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	60	from	flora	946:950	arg1	strawberry					955:964	strawberry	955:964	strawberry	955:964	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	6	61	theme	present	1328:1334	arg1	study					1336:1340	the present study	1324:1340	the present study	1324:1340	CONCLUSION The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties.
29230818	1	62	from	juices	221:226	arg1	present					199:205	present	199:205	present	199:205	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	1	63	with	investigation	382:394	arg1	deterioration					467:479	fungal deterioration	460:479	fungal deterioration over 14 days of refrigerated storage	460:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	3	64	theme	sucrose	888:894	arg1	growth					904:909	the growth	900:909	the growth of yeasts and molds (main spoilage flora in strawberry)	900:965	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	3	64	theme	sucrose	888:894	arg1	consumption					873:883	the consumption	869:883	the consumption of sucrose	869:894	When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control).
29230818	0	65	theme	strawberry	85:94	arg1	composition					34:44	carbohydrate composition	21:44	carbohydrate composition	21:44	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	0	65	theme	strawberry	85:94	arg1	deterioration					57:69	fungal deterioration	50:69	fungal deterioration	50:69	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	1	66	theme	fungal	460:465	arg1	deterioration					467:479	fungal deterioration	460:479	fungal deterioration over 14 days of refrigerated storage	460:516	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	4	67	theme	mold	1202:1205	arg1	growth					1207:1212	mold growth	1202:1212	mold growth	1202:1212	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
29230818	0	68	theme	functional	74:83	arg1	strawberry					85:94	functional strawberry	74:94	functional strawberry	74:94	Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.
29230818	1	69	theme	main	180:183	arg1	carbohydrates					185:197	the main carbohydrates	176:197	the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound)	176:347	BACKGROUND The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage.
29230818	4	70	theme	treated	1095:1101	arg1	sample					1103:1108	the treated sample	1091:1108	the treated sample	1091:1108	By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth.
30646526	0	0	theme	Sweetener	93:101	arg1	Characterization					24:39	Characterization	24:39	Characterization	24:39	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	0	0	theme	Sweetener	93:101	arg1	Synthesis					10:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	0	1	theme	Derivative	82:91	arg1	Sweetener					93:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	6	2	theme	enhanced	1038:1045	arg1	solubility					1047:1056	enhanced solubility	1038:1056	enhanced solubility	1038:1056	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	3	3	theme	beverage	494:501	arg1	products					503:510	food and beverage products	485:510	food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	485:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	4	from	applications	469:480	arg1	products					503:510	food and beverage products	485:510	food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	485:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	5	theme	low	523:525	arg1	sweetness					527:535	its low sweetness	519:535	its low sweetness	519:535	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	6	theme	high	541:544	arg1	bitterness					556:565	high lingering bitterness	541:565	high lingering bitterness	541:565	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	6	7	theme	Sensory	929:935	arg1	analysis					937:944	Sensory analysis	929:944	Sensory analysis	929:944	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	3	8	theme	third	360:364	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	8	theme	third	360:364	arg1	glycoside					389:397	the third most prevalent steviol glycoside	356:397	the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant	356:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	4	9	theme	C	730:730	arg1	profile					715:721	the taste profile	705:721	the taste profile of reb C	705:730	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	1	10	theme	market	189:194	arg1	place					196:200	the market place	185:200	the market place	185:200	Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place.
30646526	3	11	dep	the	402:404	arg1	leaves					406:411	leaves	406:411	leaves	406:411	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	1	12	from	sources	156:162	arg1	sweeteners					132:141	Zero-calorie high-intensity sweeteners	104:141	Zero-calorie high-intensity sweeteners from natural sources	104:162	Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place.
30646526	2	13	theme	natural	282:288	arg1	ingredients					290:300	novel natural ingredients	276:300	novel natural ingredients	276:300	This has encouraged food scientists to continue the effort to search for novel natural ingredients to satisfy consumer demand.
30646526	3	14	theme	Bertoni	438:444	arg1	plant					446:450	the Stevia rebaudiana Bertoni plant	416:450	the Stevia rebaudiana Bertoni plant	416:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	15	theme	steviol	381:387	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	15	theme	steviol	381:387	arg1	glycoside					389:397	the third most prevalent steviol glycoside	356:397	the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant	356:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	16	from	glycoside	389:397	arg1	the					402:404	the	402:404	the	402:404	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	17	theme	reb	346:348	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	17	theme	reb	346:348	arg1	C					350:350	reb C	346:350	reb C	346:350	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	18	theme	lingering	546:554	arg1	bitterness					556:565	high lingering bitterness	541:565	high lingering bitterness	541:565	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	5	19	theme	novel	802:806	arg1	derivative					829:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	0	20	theme	Enzymatic	0:8	arg1	Synthesis					10:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	4	21	theme	glucose	785:791	arg1	enzyme					761:766	Cargill's propriety enzyme	741:766	Cargill's propriety enzyme	741:766	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	4	21	theme	glucose	785:791	arg1	donor					793:797	a glucose donor	783:797	a glucose donor	783:797	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	4	21	theme	glucose	785:791	arg1	sucrose					772:778	sucrose	772:778	sucrose	772:778	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	1	22	theme	Zero-calorie	104:115	arg1	sweeteners					132:141	Zero-calorie high-intensity sweeteners	104:141	Zero-calorie high-intensity sweeteners from natural sources	104:162	Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place.
30646526	3	23	theme	Stevia	420:425	arg1	plant					446:450	the Stevia rebaudiana Bertoni plant	416:450	the Stevia rebaudiana Bertoni plant	416:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	1	24	theme	high-intensity	117:130	arg1	sweeteners					132:141	Zero-calorie high-intensity sweeteners	104:141	Zero-calorie high-intensity sweeteners from natural sources	104:162	Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place.
30646526	3	25	theme	prevalent	371:379	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	25	theme	prevalent	371:379	arg1	glycoside					389:397	the third most prevalent steviol glycoside	356:397	the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant	356:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	5	26	theme	NMR	911:913	arg1	spectroscopy					915:926	NMR spectroscopy	911:926	NMR spectroscopy	911:926	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	2	27	theme	consumer	313:320	arg1	demand					322:327	consumer demand	313:327	consumer demand	313:327	This has encouraged food scientists to continue the effort to search for novel natural ingredients to satisfy consumer demand.
30646526	5	28	theme	reb	823:825	arg1	derivative					829:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	3	29	theme	food	485:488	arg1	products					503:510	food and beverage products	485:510	food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	485:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	30	theme	other	579:583	arg1	A					632:632	rebaudioside A	619:632	rebaudioside A (reb A)	619:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	30	theme	other	579:583	arg1	glycosides					599:608	other major steviol glycosides	579:608	other major steviol glycosides	579:608	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	5	31	theme	α-1→6-glucosyl	808:821	arg1	derivative					829:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	3	32	theme	rebaudiana	427:436	arg1	plant					446:450	the Stevia rebaudiana Bertoni plant	416:450	the Stevia rebaudiana Bertoni plant	416:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	33	contain	has	457:459	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	33	contain	has	457:459	arg1	C					350:350	reb C	346:350	reb C	346:350	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	33	contain	has	457:459	arg1	glycoside					389:397	the third most prevalent steviol glycoside	356:397	the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant	356:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	33	contain	has	457:459	arg2	applications					469:480	limited applications	461:480	limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	461:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	34	theme	major	585:589	arg1	A					632:632	rebaudioside A	619:632	rebaudioside A (reb A)	619:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	34	theme	major	585:589	arg1	glycosides					599:608	other major steviol glycosides	579:608	other major steviol glycosides	579:608	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	6	35	theme	C	977:977	arg1	derivative					979:988	this new reb C derivative	964:988	this new reb C derivative	964:988	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	0	36	theme	α-1→6-Glucosyl	52:65	arg1	Sweetener					93:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	3	37	theme	rebaudioside	619:630	arg1	A					632:632	rebaudioside A	619:632	rebaudioside A (reb A)	619:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	37	theme	rebaudioside	619:630	arg1	A					639:639	reb A	635:639	reb A	635:639	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	4	38	theme	enzyme	665:670	arg1	strategy					685:692	a new enzyme modification strategy	659:692	a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor	659:797	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	0	39	theme	Novel	46:50	arg1	Sweetener					93:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	3	40	theme	Rebaudioside	330:341	arg1	C					343:343	Rebaudioside C	330:343	Rebaudioside C (reb C)	330:351	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	40	theme	Rebaudioside	330:341	arg1	C					350:350	reb C	346:350	reb C	346:350	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	40	theme	Rebaudioside	330:341	arg1	glycoside					389:397	the third most prevalent steviol glycoside	356:397	the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant	356:450	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	1	41	theme	natural	148:154	arg1	sources					156:162	natural sources	148:162	natural sources	148:162	Zero-calorie high-intensity sweeteners from natural sources perform very well in the market place.
30646526	2	42	theme	food	223:226	arg1	scientists					228:237	food scientists	223:237	food scientists	223:237	This has encouraged food scientists to continue the effort to search for novel natural ingredients to satisfy consumer demand.
30646526	2	43	theme	novel	276:280	arg1	ingredients					290:300	novel natural ingredients	276:300	novel natural ingredients	276:300	This has encouraged food scientists to continue the effort to search for novel natural ingredients to satisfy consumer demand.
30646526	4	44	theme	propriety	751:759	arg1	enzyme					761:766	Cargill's propriety enzyme	741:766	Cargill's propriety enzyme	741:766	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	4	44	theme	propriety	751:759	arg1	donor					793:797	a glucose donor	783:797	a glucose donor	783:797	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	4	44	theme	propriety	751:759	arg1	sucrose					772:778	sucrose	772:778	sucrose	772:778	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	3	45	theme	limited	461:467	arg1	applications					469:480	limited applications	461:480	limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	461:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	4	46	theme	taste	709:713	arg1	profile					715:721	the taste profile	705:721	the taste profile of reb C	705:730	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	6	47	theme	reb	973:975	arg1	derivative					979:988	this new reb C derivative	964:988	this new reb C derivative	964:988	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	4	48	theme	reb	726:728	arg1	C					730:730	reb C	726:730	reb C	726:730	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	3	49	theme	reb	635:637	arg1	A					632:632	rebaudioside A	619:632	rebaudioside A (reb A)	619:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	49	theme	reb	635:637	arg1	A					639:639	reb A	635:639	reb A	635:639	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	4	50	theme	modification	672:683	arg1	strategy					685:692	a new enzyme modification strategy	659:692	a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor	659:797	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30646526	6	51	theme	new	969:971	arg1	derivative					979:988	this new reb C derivative	964:988	this new reb C derivative	964:988	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	0	52	theme	C	80:80	arg1	Sweetener					93:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	5	53	theme	C	827:827	arg1	derivative					829:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative	800:838	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	3	54	theme	steviol	591:597	arg1	A					632:632	rebaudioside A	619:632	rebaudioside A (reb A)	619:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	3	54	theme	steviol	591:597	arg1	glycosides					599:608	other major steviol glycosides	579:608	other major steviol glycosides	579:608	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	5	55	theme	mass	889:892	arg1	spectrometry					894:905	mass spectrometry	889:905	mass spectrometry	889:905	A novel α-1→6-glucosyl reb C derivative was produced and its structure was elucidated by mass spectrometry and NMR spectroscopy.
30646526	6	56	theme	reduced	1014:1020	arg1	bitterness					1022:1031	reduced bitterness	1014:1031	reduced bitterness	1014:1031	Sensory analysis demonstrated that this new reb C derivative has improved sweetness, reduced bitterness, and enhanced solubility in water.
30646526	0	57	theme	Rebaudioside	67:78	arg1	Sweetener					93:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener	44:101	Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.
30646526	3	58	theme	due	512:514	arg1	products					503:510	food and beverage products	485:510	food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A)	485:640	Rebaudioside C (reb C) is the third most prevalent steviol glycoside in the leaves of the Stevia rebaudiana Bertoni plant, but has limited applications in food and beverage products due to its low sweetness and high lingering bitterness compared to other major steviol glycosides, such as rebaudioside A (reb A).
30646526	4	59	theme	new	661:663	arg1	strategy					685:692	a new enzyme modification strategy	659:692	a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor	659:797	Here we present a new enzyme modification strategy to improve the taste profile of reb C by using Cargill's propriety enzyme and sucrose as a glucose donor.
30021396	2	0	theme	injectable	371:380	arg1	hydrogels					418:426	injectable and degradable polysaccharide-based hydrogels	371:426	injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs)	371:525	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	7	1	theme	antibacterial	1395:1407	arg1	evaluation					1409:1418	antibacterial evaluation	1395:1418	antibacterial evaluation	1395:1418	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	1	theme	antibacterial	1395:1407	arg1	drugs					1365:1369	drugs	1365:1369	drugs release calculation and antibacterial evaluation	1365:1418	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	8	2	theme	dynamic	1489:1495	arg1	equilibrium					1497:1507	dynamic equilibrium	1489:1507	dynamic equilibrium of the Schiff-base linkages	1489:1535	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	0	3	theme	drug	90:93	arg1	delivery					95:102	drug delivery	90:102	drug delivery	90:102	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	6	4	theme	in	1154:1155	arg1	degradation					1163:1173	in vitro degradation	1154:1173	in vitro degradation	1154:1173	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	1	5	theme	bone	174:177	arg1	purpose					192:198	bone regeneration purpose	174:198	bone regeneration purpose	174:198	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	1	6	theme	local	285:289	arg1	delivery					296:303	local drug delivery	285:303	local drug delivery	285:303	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	0	7	theme	bone	108:111	arg1	engineering					120:130	bone tissue engineering	108:130	bone tissue engineering	108:130	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	7	8	theme	release	1371:1377	arg1	drugs					1365:1369	drugs	1365:1369	drugs release calculation and antibacterial evaluation	1365:1418	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	8	theme	release	1371:1377	arg1	calculation					1379:1389	release calculation	1371:1389	release calculation	1371:1389	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	4	9	theme	6.62 μm	874:880	arg1	diameter					862:869	an average diameter	851:869	an average diameter of 6.62 μm	851:880	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	9	10	theme	great	1609:1613	arg1	potential					1615:1623	great potential	1609:1623	great potential for drug delivery and regeneration of irregular bone defects	1609:1684	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	4	11	theme	loaded	789:794	arg1	CMs					796:798	tetracycline hydrochloride (TH) loaded CMs	757:798	tetracycline hydrochloride (TH) loaded CMs	757:798	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	1	12	theme	drug	291:294	arg1	delivery					296:303	local drug delivery	285:303	local drug delivery	285:303	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	5	13	theme	injectable	1027:1036	arg1	scaffolds					1042:1050	injectable gel scaffolds	1027:1050	injectable gel scaffolds for imitation of bone niche	1027:1078	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	1	14	theme	delivery	296:303	arg1	applications					305:316	tissue regeneration and local drug delivery applications	261:316	tissue regeneration and local drug delivery applications	261:316	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	6	15	theme	gel	1182:1184	arg1	scaffolds					1186:1194	the gel scaffolds	1178:1194	the gel scaffolds	1178:1194	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	3	16	theme	cross-linking	575:587	arg1	mechanism					562:570	The mechanism	558:570	The mechanism of cross-linking	558:587	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	1	17	theme	regeneration	179:190	arg1	purpose					192:198	bone regeneration purpose	174:198	bone regeneration purpose	174:198	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	0	18	theme	tissue	113:118	arg1	engineering					120:130	bone tissue engineering	108:130	bone tissue engineering	108:130	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	5	19	theme	mechanical	908:917	arg1	properties					919:928	bioactive and mechanical properties	894:928	bioactive and mechanical properties	894:928	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	0	20	theme	polysaccharide	11:24	arg1	hydrogel					26:33	Injectable polysaccharide hydrogel	0:33	Injectable polysaccharide hydrogel	0:33	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	8	21	theme	gel	1438:1440	arg1	scaffolds					1442:1450	the gel scaffolds	1434:1450	the gel scaffolds	1434:1450	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	8	21	theme	gel	1438:1440	arg1	due					1482:1484	due	1482:1484	due	1482:1484	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	7	22	theme	sustained	1296:1304	arg1	release					1311:1317	good sustained drug release	1291:1317	good sustained drug release	1291:1317	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	9	23	theme	composite	1580:1588	arg1	scaffolds					1594:1602	the prepared composite gel scaffolds	1567:1602	the prepared composite gel scaffolds	1567:1602	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	0	24	theme	Injectable	0:9	arg1	hydrogel					26:33	Injectable polysaccharide hydrogel	0:33	Injectable polysaccharide hydrogel	0:33	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	4	25	with	reaction	837:844	arg1	diameter					862:869	an average diameter	851:869	an average diameter of 6.62 μm	851:880	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	8	26	theme	linkages	1528:1535	arg1	equilibrium					1497:1507	dynamic equilibrium	1489:1507	dynamic equilibrium of the Schiff-base linkages	1489:1535	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	5	27	theme	polysaccharide-based	989:1008	arg1	hydrogel					1010:1017	the polysaccharide-based hydrogel	985:1017	the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche	985:1078	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	1	28	theme	injectable	201:210	arg1	hydrogels					212:220	injectable hydrogels	201:220	injectable hydrogels	201:220	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	7	29	theme	good	1291:1294	arg1	release					1311:1317	good sustained drug release	1291:1317	good sustained drug release	1291:1317	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	5	30	theme	niche	1074:1078	arg1	imitation					1056:1064	imitation	1056:1064	imitation of bone niche	1056:1078	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	8	31	located	found	1457:1461	arg2	due					1482:1484	due	1482:1484	due	1482:1484	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	8	31	located	found	1457:1461	arg1	addition					1424:1431	addition	1424:1431	addition	1424:1431	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	8	31	located	found	1457:1461	arg2	scaffolds					1442:1450	the gel scaffolds	1434:1450	the gel scaffolds	1434:1450	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	5	32	theme	gel	1038:1040	arg1	scaffolds					1042:1050	injectable gel scaffolds	1027:1050	injectable gel scaffolds for imitation of bone niche	1027:1078	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	6	33	theme	CMs	1235:1237	arg1	contents					1239:1246	CMs contents	1235:1246	CMs contents	1235:1246	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	3	34	theme	Schiff-base	610:620	arg1	reaction					622:629	the Schiff-base reaction	606:629	the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively	606:739	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	9	35	theme	gel	1590:1592	arg1	scaffolds					1594:1602	the prepared composite gel scaffolds	1567:1602	the prepared composite gel scaffolds	1567:1602	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	2	36	theme	degradable	386:395	arg1	hydrogels					418:426	injectable and degradable polysaccharide-based hydrogels	371:426	injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs)	371:525	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	3	37	theme	oxidized	702:709	arg1	OAlg					721:724	OAlg	721:724	OAlg	721:724	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	37	theme	oxidized	702:709	arg1	alginate					711:718	oxidized alginate	702:718	oxidized alginate (OAlg)	702:725	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	38	theme	aldehyde	649:656	arg1	OAlg					721:724	OAlg	721:724	OAlg	721:724	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	38	theme	aldehyde	649:656	arg1	CMCS					692:695	CMCS	692:695	CMCS	692:695	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	38	theme	aldehyde	649:656	arg1	groups					658:663	amino and aldehyde groups	639:663	groups	658:663	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	38	theme	aldehyde	649:656	arg1	chitosan					682:689	carboxymethyl chitosan	668:689	carboxymethyl chitosan (CMCS)	668:696	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	38	theme	aldehyde	649:656	arg1	alginate					711:718	oxidized alginate	702:718	oxidized alginate (OAlg)	702:725	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	2	39	theme	polysaccharide-based	397:416	arg1	hydrogels					418:426	injectable and degradable polysaccharide-based hydrogels	371:426	injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs)	371:525	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	2	40	theme	hydroxyapatite	451:464	arg1	nanoparticles					472:484	hydroxyapatite (HAp) nanoparticles	451:484	hydroxyapatite (HAp) nanoparticles	451:484	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	6	41	theme	swelling	1135:1142	arg1	ratio					1144:1148	swelling ratio	1135:1148	swelling ratio	1135:1148	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	4	42	theme	average	854:860	arg1	diameter					862:869	an average diameter	851:869	an average diameter of 6.62 μm	851:880	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	0	43	with	embedded	35:42	arg1	hydroxyapatite					49:62	hydroxyapatite	49:62	hydroxyapatite	49:62	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	0	43	with	embedded	35:42	arg1	carbonate					76:84	calcium carbonate	68:84	calcium carbonate	68:84	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	1	44	theme	increasing	237:246	arg1	attention					248:256	increasing attention	237:256	increasing attention	237:256	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	0	45	theme	calcium	68:74	arg1	carbonate					76:84	calcium carbonate	68:84	calcium carbonate	68:84	Injectable polysaccharide hydrogel embedded with hydroxyapatite and calcium carbonate for drug delivery and bone tissue engineering.
30021396	9	46	theme	prepared	1571:1578	arg1	scaffolds					1594:1602	the prepared composite gel scaffolds	1567:1602	the prepared composite gel scaffolds	1567:1602	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	7	47	theme	gel	1273:1275	arg1	scaffolds					1277:1285	the composite gel scaffolds	1259:1285	the composite gel scaffolds	1259:1285	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	3	48	theme	amino	639:643	arg1	OAlg					721:724	OAlg	721:724	OAlg	721:724	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	48	theme	amino	639:643	arg1	CMCS					692:695	CMCS	692:695	CMCS	692:695	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	48	theme	amino	639:643	arg1	groups					658:663	amino and aldehyde groups	639:663	groups	658:663	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	48	theme	amino	639:643	arg1	chitosan					682:689	carboxymethyl chitosan	668:689	carboxymethyl chitosan (CMCS)	668:696	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	48	theme	amino	639:643	arg1	alginate					711:718	oxidized alginate	702:718	oxidized alginate (OAlg)	702:725	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	4	49	theme	tetracycline	757:768	arg1	TH					785:786	TH	785:786	TH	785:786	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	4	49	theme	tetracycline	757:768	arg1	hydrochloride					770:782	tetracycline hydrochloride	757:782	tetracycline hydrochloride (TH) loaded CMs	757:798	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	2	50	theme	carbonate	498:506	arg1	microspheres					508:519	calcium carbonate microspheres	490:519	calcium carbonate microspheres (CMs)	490:525	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	2	50	theme	carbonate	498:506	arg1	CMs					522:524	CMs	522:524	CMs	522:524	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	6	51	theme	mechanical	1112:1121	arg1	properties					1123:1132	mechanical properties	1112:1132	mechanical properties	1112:1132	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	8	52	theme	Schiff-base	1516:1526	arg1	linkages					1528:1535	the Schiff-base linkages	1512:1535	the Schiff-base linkages	1512:1535	In addition, the gel scaffolds were found to be self-healing due to dynamic equilibrium of the Schiff-base linkages.
30021396	6	53	dep	in	1154:1155	arg1	vitro					1157:1161	vitro	1157:1161	vitro	1157:1161	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	7	54	theme	antibacterial	1323:1335	arg1	properties					1337:1346	antibacterial properties	1323:1346	antibacterial properties	1323:1346	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	5	55	theme	bone	1069:1072	arg1	niche					1074:1078	bone niche	1069:1078	bone niche	1069:1078	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	9	56	theme	irregular	1663:1671	arg1	defects					1678:1684	irregular bone defects	1663:1684	irregular bone defects	1663:1684	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	9	57	theme	defects	1678:1684	arg1	regeneration					1647:1658	regeneration	1647:1658	regeneration of irregular bone defects	1647:1684	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	9	57	theme	defects	1678:1684	arg1	delivery					1634:1641	drug delivery	1629:1641	drug delivery	1629:1641	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	3	58	theme	chitosan	682:689	arg1	OAlg					721:724	OAlg	721:724	OAlg	721:724	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	58	theme	chitosan	682:689	arg1	CMCS					692:695	CMCS	692:695	CMCS	692:695	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	58	theme	chitosan	682:689	arg1	groups					658:663	amino and aldehyde groups	639:663	groups	658:663	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	58	theme	chitosan	682:689	arg1	chitosan					682:689	carboxymethyl chitosan	668:689	carboxymethyl chitosan (CMCS)	668:696	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	58	theme	chitosan	682:689	arg1	alginate					711:718	oxidized alginate	702:718	oxidized alginate (OAlg)	702:725	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	7	59	dep	drugs	1365:1369	arg1	evaluation					1409:1418	antibacterial evaluation	1395:1418	antibacterial evaluation	1395:1418	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	59	dep	drugs	1365:1369	arg1	drugs					1365:1369	drugs	1365:1369	drugs release calculation and antibacterial evaluation	1365:1418	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	59	dep	drugs	1365:1369	arg1	calculation					1379:1389	release calculation	1371:1389	release calculation	1371:1389	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	2	60	theme	facile	346:351	arg1	method					353:358	a facile method	344:358	a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition	344:555	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	6	61	theme	gelation	1085:1092	arg1	time					1094:1097	The gelation time	1081:1097	The gelation time	1081:1097	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	3	62	theme	alginate	711:718	arg1	OAlg					721:724	OAlg	721:724	OAlg	721:724	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	62	theme	alginate	711:718	arg1	CMCS					692:695	CMCS	692:695	CMCS	692:695	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	62	theme	alginate	711:718	arg1	groups					658:663	amino and aldehyde groups	639:663	groups	658:663	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	62	theme	alginate	711:718	arg1	chitosan					682:689	carboxymethyl chitosan	668:689	carboxymethyl chitosan (CMCS)	668:696	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	62	theme	alginate	711:718	arg1	alginate					711:718	oxidized alginate	702:718	oxidized alginate (OAlg)	702:725	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	4	63	theme	precipitation	823:835	arg1	reaction					837:844	the precipitation reaction	819:844	the precipitation reaction with an average diameter of 6.62 μm	819:880	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	5	64	contain	containing	948:957	arg1	nano-HAp					931:938	nano-HAp	931:938	nano-HAp	931:938	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	5	64	contain	containing	948:957	arg2	TH					959:960	TH	959:960	TH	959:960	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	4	65	theme	hydrochloride	770:782	arg1	CMs					796:798	tetracycline hydrochloride (TH) loaded CMs	757:798	tetracycline hydrochloride (TH) loaded CMs	757:798	Synchronously, tetracycline hydrochloride (TH) loaded CMs were fabricated by the precipitation reaction with an average diameter of 6.62 μm.
30021396	7	66	theme	drug	1306:1309	arg1	release					1311:1317	good sustained drug release	1291:1317	good sustained drug release	1291:1317	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	67	theme	composite	1263:1271	arg1	scaffolds					1277:1285	the composite gel scaffolds	1259:1285	the composite gel scaffolds	1259:1285	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	5	68	theme	bioactive	894:902	arg1	properties					919:928	bioactive and mechanical properties	894:928	bioactive and mechanical properties	894:928	To enhance bioactive and mechanical properties, nano-HAp and CMs containing TH were encapsulated into the polysaccharide-based hydrogel to form injectable gel scaffolds for imitation of bone niche.
30021396	7	69	contain	had	1287:1289	arg2	properties					1337:1346	antibacterial properties	1323:1346	antibacterial properties	1323:1346	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	69	contain	had	1287:1289	arg1	scaffolds					1277:1285	the composite gel scaffolds	1259:1285	the composite gel scaffolds	1259:1285	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	7	69	contain	had	1287:1289	arg2	release					1311:1317	good sustained drug release	1291:1317	good sustained drug release	1291:1317	Moreover, the composite gel scaffolds had good sustained drug release and antibacterial properties, as confirmed by drugs release calculation and antibacterial evaluation.
30021396	1	70	theme	progressive	145:155	arg1	requirements					157:168	the progressive requirements	141:168	the progressive requirements for bone regeneration purpose	141:198	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	9	71	theme	drug	1629:1632	arg1	delivery					1634:1641	drug delivery	1629:1641	drug delivery	1629:1641	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	1	72	theme	tissue	261:266	arg1	regeneration					268:279	tissue regeneration	261:279	tissue regeneration	261:279	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30021396	2	73	theme	physiological	533:545	arg1	condition					547:555	physiological condition	533:555	physiological condition	533:555	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	6	74	theme	scaffolds	1186:1194	arg1	morphology					1100:1109	morphology	1100:1109	morphology	1100:1109	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	6	74	theme	scaffolds	1186:1194	arg1	properties					1123:1132	mechanical properties	1112:1132	mechanical properties	1112:1132	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	6	74	theme	scaffolds	1186:1194	arg1	time					1094:1097	The gelation time	1081:1097	The gelation time	1081:1097	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	6	74	theme	scaffolds	1186:1194	arg1	ratio					1144:1148	swelling ratio	1135:1148	swelling ratio	1135:1148	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	6	74	theme	scaffolds	1186:1194	arg1	degradation					1163:1173	in vitro degradation	1154:1173	in vitro degradation	1154:1173	The gelation time, morphology, mechanical properties, swelling ratio and in vitro degradation of the gel scaffolds could be controlled by varying HAp and CMs contents.
30021396	3	75	theme	carboxymethyl	668:680	arg1	CMCS					692:695	CMCS	692:695	CMCS	692:695	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	3	75	theme	carboxymethyl	668:680	arg1	chitosan					682:689	carboxymethyl chitosan	668:689	carboxymethyl chitosan (CMCS)	668:696	The mechanism of cross-linking is attributed to the Schiff-base reaction between amino and aldehyde groups of carboxymethyl chitosan (CMCS) and oxidized alginate (OAlg), respectively.
30021396	2	76	theme	calcium	490:496	arg1	microspheres					508:519	calcium carbonate microspheres	490:519	calcium carbonate microspheres (CMs)	490:525	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	2	76	theme	calcium	490:496	arg1	CMs					522:524	CMs	522:524	CMs	522:524	In this study, we report a facile method to prepare injectable and degradable polysaccharide-based hydrogels doubly integrated with hydroxyapatite (HAp) nanoparticles and calcium carbonate microspheres (CMs) under physiological condition.
30021396	9	77	theme	bone	1673:1676	arg1	defects					1678:1684	irregular bone defects	1663:1684	irregular bone defects	1663:1684	These results suggested that the prepared composite gel scaffolds hold great potential for drug delivery and regeneration of irregular bone defects.
30021396	1	78	theme	regeneration	268:279	arg1	applications					305:316	tissue regeneration and local drug delivery applications	261:316	tissue regeneration and local drug delivery applications	261:316	To meet the progressive requirements for bone regeneration purpose, injectable hydrogels have attracted increasing attention in tissue regeneration and local drug delivery applications.
30827585	2	0	theme	antiglycation	122:134	arg1	activities					136:145	potential antiglycation activities	112:145	potential antiglycation activities	112:145	major): Molecular characterization and potential antiglycation activities.
30827585	9	1	theme	ultrasound-assisted	875:893	arg1	degradation					905:915	ultrasound-assisted enzymatic degradation	875:915	ultrasound-assisted enzymatic degradation	875:915	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	2	2	theme	potential	112:120	arg1	activities					136:145	potential antiglycation activities	112:145	potential antiglycation activities	112:145	major): Molecular characterization and potential antiglycation activities.
30827585	9	3	theme	enzymatic	895:903	arg1	degradation					905:915	ultrasound-assisted enzymatic degradation	875:915	ultrasound-assisted enzymatic degradation	875:915	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	9	4	theme	MM-POS	854:859	arg1	analysis					842:849	Composition analysis	830:849	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation	830:915	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	8	5	theme	antiglycation	751:763	arg1	activity					765:772	stronger antiglycation activity	742:772	stronger antiglycation activity	742:772	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	5	6	theme	DEAE	304:307	arg1	chromatography					322:335	DEAE cellulose-52 chromatography	304:335	DEAE cellulose-52 chromatography	304:335	major) and a salt-eluted polysaccharide (SPS-2) was fractionated by DEAE cellulose-52 chromatography.
30827585	9	7	theme	Composition	830:840	arg1	analysis					842:849	Composition analysis	830:849	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation	830:915	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	6	8	theme	infrared	387:394	arg1	analysis					404:411	Fourier-transform infrared (FT-IR) analysis	369:411	Fourier-transform infrared (FT-IR) analysis	369:411	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	9	9	theme	10.6	1041:1044	arg1	%					1045:1045	%	1045:1045	%	1045:1045	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	7	10	theme	SPS-2	655:659	arg1	products					673:680	SPS-2 degradation products	655:680	SPS-2 degradation products	655:680	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	7	11	theme	antiglycation	626:638	arg1	activity					640:647	the highest antiglycation activity	614:647	the highest antiglycation activity	614:647	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	6	12	theme	Fourier-transform	369:385	arg1	analysis					404:411	Fourier-transform infrared (FT-IR) analysis	369:411	Fourier-transform infrared (FT-IR) analysis	369:411	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	8	13	contain	had	738:740	arg1	MM-POS					697:702	the MM-POS	693:702	the MM-POS obtained by enzymatic degradation	693:736	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	8	13	contain	had	738:740	arg2	activity					765:772	stronger antiglycation activity	742:772	stronger antiglycation activity	742:772	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	7	14	theme	degradation	661:671	arg1	products					673:680	SPS-2 degradation products	655:680	SPS-2 degradation products	655:680	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	6	15	theme	galacturonic	454:465	arg1	acid					467:470	72.3% galacturonic acid	448:470	72.3% galacturonic acid	448:470	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	7	16	theme	activity	520:527	arg1	results					495:501	The results	491:501	The results of antiglycation activity in vitro	491:536	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	9	17	theme	polygalacturonans	929:945	arg1	content					947:953	polygalacturonans content	929:953	polygalacturonans content	929:953	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	8	18	theme	stronger	742:749	arg1	activity					765:772	stronger antiglycation activity	742:772	stronger antiglycation activity	742:772	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	6	19	theme	%	452:452	arg1	acid					467:470	72.3% galacturonic acid	448:470	72.3% galacturonic acid	448:470	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	9	20	theme	enzymatic	1131:1139	arg1	degradation					1141:1151	enzymatic degradation	1131:1151	enzymatic degradation	1131:1151	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	8	21	theme	enzymatic	807:815	arg1	degradation					817:827	ultrasonic assisted enzymatic degradation	787:827	ultrasonic assisted enzymatic degradation	787:827	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	9	22	theme	22.7	1050:1053	arg1	%					1045:1045	%	1045:1045	%	1045:1045	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	6	23	theme	72.3	448:451	arg1	%					452:452	%	452:452	%	452:452	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	6	24	theme	Monosaccharide	338:351	arg1	composition					353:363	Monosaccharide composition	338:363	Monosaccharide composition	338:363	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	8	25	theme	enzymatic	716:724	arg1	degradation					726:736	enzymatic degradation	716:736	enzymatic degradation	716:736	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	7	26	dep	weight	571:576	arg1	MM-POS					602:607	MM-POS	602:607	MM-POS	602:607	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	7	26	dep	weight	571:576	arg1	oligosaccharide					585:599	pectin oligosaccharide	578:599	the medium molecular weight pectin oligosaccharide (MM-POS)	550:608	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	2	27	dep	characterization	91:106	arg1	major					73:77	major	73:77	major	73:77	major): Molecular characterization and potential antiglycation activities.
30827585	9	28	dep	%	1068:1068	arg1	to					1070:1071	to	1070:1071	to	1070:1071	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	6	29	contain	contained	438:446	arg2	acid					467:470	72.3% galacturonic acid	448:470	72.3% galacturonic acid	448:470	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	6	29	contain	contained	438:446	arg1	SPS-2					432:436	the SPS-2	428:436	the SPS-2	428:436	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	7	30	theme	antiglycation	506:518	arg1	activity					520:527	antiglycation activity	506:527	antiglycation activity in vitro	506:536	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	6	31	theme	FT-IR	397:401	arg1	analysis					404:411	Fourier-transform infrared (FT-IR) analysis	369:411	Fourier-transform infrared (FT-IR) analysis	369:411	Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin.
30827585	9	32	dep	increased	1026:1034	arg1	compared					1094:1101	compared	1094:1101	compared to the MM-POS obtained from enzymatic degradation	1094:1151	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	9	33	theme	3.5	1065:1067	arg1	%					1068:1068	%	1068:1068	%	1068:1068	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	9	34	theme	%	974:974	arg1	%					983:983	85.1% to 61.9%	970:983	85.1% to 61.9%	970:983	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	5	35	theme	cellulose-52	309:320	arg1	chromatography					322:335	DEAE cellulose-52 chromatography	304:335	DEAE cellulose-52 chromatography	304:335	major) and a salt-eluted polysaccharide (SPS-2) was fractionated by DEAE cellulose-52 chromatography.
30827585	7	36	theme	pectin	578:583	arg1	MM-POS					602:607	MM-POS	602:607	MM-POS	602:607	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	7	36	theme	pectin	578:583	arg1	oligosaccharide					585:599	pectin oligosaccharide	578:599	the medium molecular weight pectin oligosaccharide (MM-POS)	550:608	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	9	37	dep	%	983:983	arg1	to					976:977	to	976:977	to	976:977	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	2	38	theme	Molecular	81:89	arg1	characterization					91:106	Molecular characterization	81:106	Molecular characterization	81:106	major): Molecular characterization and potential antiglycation activities.
30827585	9	39	dep	%	1045:1045	arg1	to					1047:1048	to	1047:1048	to	1047:1048	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	5	40	theme	salt-eluted	249:259	arg1	SPS-2					277:281	SPS-2	277:281	SPS-2	277:281	major) and a salt-eluted polysaccharide (SPS-2) was fractionated by DEAE cellulose-52 chromatography.
30827585	5	40	theme	salt-eluted	249:259	arg1	polysaccharide					261:274	a salt-eluted polysaccharide	247:274	a salt-eluted polysaccharide (SPS-2)	247:282	major) and a salt-eluted polysaccharide (SPS-2) was fractionated by DEAE cellulose-52 chromatography.
30827585	7	41	theme	medium	554:559	arg1	weight					571:576	the medium molecular weight	550:576	the medium molecular weight pectin oligosaccharide (MM-POS)	550:608	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	9	42	theme	13.2	1073:1076	arg1	%					1068:1068	%	1068:1068	%	1068:1068	Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation.
30827585	8	43	theme	ultrasonic	787:796	arg1	degradation					817:827	ultrasonic assisted enzymatic degradation	787:827	ultrasonic assisted enzymatic degradation	787:827	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	7	44	theme	molecular	561:569	arg1	weight					571:576	the medium molecular weight	550:576	the medium molecular weight pectin oligosaccharide (MM-POS)	550:608	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	10	45	theme	agents	1290:1295	arg1	exploitation					1260:1271	the exploitation	1256:1271	the exploitation of antiglycation agents	1256:1295	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	8	46	theme	assisted	798:805	arg1	degradation					817:827	ultrasonic assisted enzymatic degradation	787:827	ultrasonic assisted enzymatic degradation	787:827	Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation.
30827585	10	47	from	application	1206:1216	arg1	field					1238:1242	the food field	1229:1242	the food field	1229:1242	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	3	48	dep	extracted	177:185	arg1	Bunge					224:228	Bunge	224:228	Bunge	224:228	The crude polysaccharide was extracted from hawthorn (Crataegus pinnatifida Bunge.
30827585	10	49	theme	food	1233:1236	arg1	field					1238:1242	the food field	1229:1242	the food field	1229:1242	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	7	50	theme	highest	618:624	arg1	activity					640:647	the highest antiglycation activity	614:647	the highest antiglycation activity	614:647	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	3	51	theme	crude	152:156	arg1	polysaccharide					158:171	The crude polysaccharide	148:171	The crude polysaccharide	148:171	The crude polysaccharide was extracted from hawthorn (Crataegus pinnatifida Bunge.
30827585	0	52	dep	oligosaccharides	7:22	arg1	Bunge					61:65	Bunge	61:65	Bunge	61:65	Pectin oligosaccharides from hawthorn (Crataegus pinnatifida Bunge.
30827585	10	53	theme	theoretical	1180:1190	arg1	basis					1192:1196	a theoretical basis	1178:1196	a theoretical basis for the application of POSs in the food field	1178:1242	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	10	54	theme	antiglycation	1276:1288	arg1	agents					1290:1295	antiglycation agents	1276:1295	antiglycation agents	1276:1295	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	10	55	theme	POSs	1221:1224	arg1	application					1206:1216	the application	1202:1216	the application of POSs in the food field	1202:1242	This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
30827585	7	56	contain	had	610:612	arg2	activity					640:647	the highest antiglycation activity	614:647	the highest antiglycation activity	614:647	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
30827585	7	56	contain	had	610:612	arg1	weight					571:576	the medium molecular weight	550:576	the medium molecular weight pectin oligosaccharide (MM-POS)	550:608	The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products.
31887941	1	0	theme	products	331:338	arg1	deterioration					340:352	the bakery products deterioration	320:352	the bakery products deterioration	320:352	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	7	1	theme	oxygen	1009:1014	arg1	permeability					1016:1027	oxygen permeability	1009:1027	oxygen permeability	1009:1027	Water permeability slightly increased, as expected, whereas oxygen permeability decreased.
31887941	4	2	theme	pH	712:713	arg1	values					715:720	different pH values	702:720	different pH values	702:720	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	0	3	theme	chemico-physical	92:107	arg1	properties					109:118	Antifungal and chemico-physical properties	77:118	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	1	4	theme	deterioration	340:352	arg1	responsible					305:315	responsible	305:315	responsible	305:315	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	8	5	theme	CTUN	1061:1064	arg1	sub-microparticles					1066:1083	CTUN sub-microparticles	1061:1083	CTUN sub-microparticles	1061:1083	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	4	6	theme	different	702:710	arg1	values					715:720	different pH values	702:720	different pH values	702:720	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	9	7	theme	bioactive	1259:1267	arg1	materials					1297:1305	bioactive bakery based food packaging materials	1259:1305	bioactive bakery based food packaging materials	1259:1305	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	7	8	dep	increased	977:985	arg1	whereas					1001:1007	whereas	1001:1007	whereas	1001:1007	Water permeability slightly increased, as expected, whereas oxygen permeability decreased.
31887941	5	9	theme	MBi	749:751	arg1	crystallization					753:767	MBi crystallization	749:767	MBi crystallization (DSC analysis)	749:782	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	5	9	theme	MBi	749:751	arg1	analysis					774:781	DSC analysis	770:781	DSC analysis	770:781	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	9	10	theme	bakery	1269:1274	arg1	materials					1297:1305	bioactive bakery based food packaging materials	1259:1305	bioactive bakery based food packaging materials	1259:1305	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	8	11	dep	showed	1054:1059	arg1	improved					1085:1092	improved	1085:1092	showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility	1054:1163	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	1	12	theme	active	258:263	arg1	alkaloid					236:243	an alkaloid	233:243	an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration,	233:353	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	12	theme	active	258:263	arg1	ungeremine					214:223	ungeremine	214:223	ungeremine (CTUn)	214:230	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	2	13	theme	bioactive	514:522	arg1	biocomposites					524:536	MBi/CTUn bioactive biocomposites	505:536	MBi/CTUn bioactive biocomposites	505:536	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	6	14	theme	particles	912:920	arg1	distribution					886:897	even distribution	881:897	even distribution of sub-micro particles into the polymeric matrix	881:946	Morphological analysis confirmed even distribution of sub-micro particles into the polymeric matrix.
31887941	8	15	theme	tensile	1107:1113	arg1	strength					1115:1122	tensile strength	1107:1122	tensile strength	1107:1122	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	5	16	theme	MBi	820:822	arg1	TGA					842:844	TGA	842:844	TGA	842:844	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	5	16	theme	MBi	820:822	arg1	component					831:839	MBi starch component	820:839	MBi starch component (TGA)	820:845	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	1	17	theme	tripolyphosphate	162:177	arg1	particles					193:201	chitosan (C) tripolyphosphate (T) sub-micro particles	149:201	chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration,	149:353	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	0	18	theme	films	127:131	arg1	properties					109:118	Antifungal and chemico-physical properties	77:118	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	6	19	theme	sub-micro	902:910	arg1	particles					912:920	sub-micro particles	902:920	sub-micro particles	902:920	Morphological analysis confirmed even distribution of sub-micro particles into the polymeric matrix.
31887941	8	20	theme	Tensile	1040:1046	arg1	tests					1048:1052	Tensile tests	1040:1052	Tensile tests	1040:1052	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	2	21	theme	thermoplastic	450:462	arg1	MBi					495:497	MBi	495:497	MBi	495:497	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	2	21	theme	thermoplastic	450:462	arg1	Mater-Bi					485:492	a thermoplastic starch based polymer Mater-Bi	448:492	a thermoplastic starch based polymer Mater-Bi (MBi)	448:498	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	4	22	theme	different	663:671	arg1	concentration					673:685	different concentration	663:685	different concentration of CTUn	663:693	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	0	23	theme	starch	14:19	arg1	biocomposites					62:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites	0:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	1	24	theme	external	377:384	arg1	process					408:414	external gelation crosslinking process	377:414	external gelation crosslinking process	377:414	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	9	25	theme	packaging	1287:1295	arg1	materials					1297:1305	bioactive bakery based food packaging materials	1259:1305	bioactive bakery based food packaging materials	1259:1305	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	4	26	theme	CTUn	690:693	arg1	concentration					673:685	different concentration	663:685	different concentration of CTUn	663:693	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	0	27	theme	Thermoplastic	0:12	arg1	starch					14:19	Thermoplastic starch	0:19	Thermoplastic starch	0:19	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	7	28	theme	Water	949:953	arg1	permeability					955:966	Water permeability	949:966	Water permeability	949:966	Water permeability slightly increased, as expected, whereas oxygen permeability decreased.
31887941	6	29	theme	Morphological	848:860	arg1	analysis					862:869	Morphological analysis	848:869	Morphological analysis	848:869	Morphological analysis confirmed even distribution of sub-micro particles into the polymeric matrix.
31887941	6	30	theme	even	881:884	arg1	distribution					886:897	even distribution	881:897	even distribution of sub-micro particles into the polymeric matrix	881:946	Morphological analysis confirmed even distribution of sub-micro particles into the polymeric matrix.
31887941	0	31	theme	bioactive	25:33	arg1	chitosan					35:42	bioactive chitosan	25:42	bioactive chitosan	25:42	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	5	32	theme	starch	824:829	arg1	TGA					842:844	TGA	842:844	TGA	842:844	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	5	32	theme	starch	824:829	arg1	component					831:839	MBi starch component	820:839	MBi starch component (TGA)	820:845	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	8	33	theme	films	1131:1135	arg1	rigidity					1094:1101	rigidity	1094:1101	rigidity	1094:1101	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	8	33	theme	films	1131:1135	arg1	strength					1115:1122	tensile strength	1107:1122	tensile strength	1107:1122	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	9	34	theme	based	1276:1280	arg1	materials					1297:1305	bioactive bakery based food packaging materials	1259:1305	bioactive bakery based food packaging materials	1259:1305	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	1	35	theme	sub-micro	183:191	arg1	particles					193:201	chitosan (C) tripolyphosphate (T) sub-micro particles	149:201	chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration,	149:353	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	36	theme	gelation	386:393	arg1	process					408:414	external gelation crosslinking process	377:414	external gelation crosslinking process	377:414	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	5	37	theme	component	831:839	arg1	degradation					805:815	thermal degradation	797:815	thermal degradation of MBi starch component (TGA)	797:845	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	0	38	theme	sub-microparticle	44:60	arg1	biocomposites					62:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites	0:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	5	39	theme	CTUn	723:726	arg1	particles					728:736	CTUn particles	723:736	CTUn particles	723:736	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	1	40	theme	crosslinking	395:406	arg1	process					408:414	external gelation crosslinking process	377:414	external gelation crosslinking process	377:414	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	0	41	theme	chitosan	35:42	arg1	biocomposites					62:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites	0:74	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	5	42	theme	thermal	797:803	arg1	degradation					805:815	thermal degradation	797:815	thermal degradation of MBi starch component (TGA)	797:845	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	0	43	dep	biocomposites	62:74	arg1	properties					109:118	Antifungal and chemico-physical properties	77:118	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	2	44	theme	MBi/CTUn	505:512	arg1	biocomposites					524:536	MBi/CTUn bioactive biocomposites	505:536	MBi/CTUn bioactive biocomposites	505:536	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	2	45	theme	polymer	477:483	arg1	MBi					495:497	MBi	495:497	MBi	495:497	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	2	45	theme	polymer	477:483	arg1	Mater-Bi					485:492	a thermoplastic starch based polymer Mater-Bi	448:492	a thermoplastic starch based polymer Mater-Bi (MBi)	448:498	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	1	46	theme	responsible	305:315	arg1	Penicilliumroqueforti					273:293	Penicilliumroqueforti	273:293	Penicilliumroqueforti	273:293	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	46	theme	responsible	305:315	arg1	fungus					298:303	a fungus	296:303	a fungus responsible of the bakery products deterioration	296:352	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	47	dep	tripolyphosphate	162:177	arg1	T					180:180	T	180:180	T	180:180	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	2	48	theme	based	471:475	arg1	MBi					495:497	MBi	495:497	MBi	495:497	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	2	48	theme	based	471:475	arg1	Mater-Bi					485:492	a thermoplastic starch based polymer Mater-Bi	448:492	a thermoplastic starch based polymer Mater-Bi (MBi)	448:498	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	6	49	theme	polymeric	931:939	arg1	matrix					941:946	the polymeric matrix	927:946	the polymeric matrix	927:946	Morphological analysis confirmed even distribution of sub-micro particles into the polymeric matrix.
31887941	4	50	with	films	652:656	arg1	concentration					673:685	different concentration	663:685	different concentration of CTUn	663:693	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	9	51	theme	MBi/CTUn	1175:1182	arg1	biocomposites					1184:1196	MBi/CTUn biocomposites	1175:1196	MBi/CTUn biocomposites evidenced interesting performances	1175:1231	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	1	52	contain	containing	203:212	arg2	ungeremine					214:223	ungeremine	214:223	ungeremine (CTUn)	214:230	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	52	contain	containing	203:212	arg1	particles					193:201	chitosan (C) tripolyphosphate (T) sub-micro particles	149:201	chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration,	149:353	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	52	contain	containing	203:212	arg2	alkaloid					236:243	an alkaloid	233:243	an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration,	233:353	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	1	52	contain	containing	203:212	arg2	CTUn					226:229	CTUn	226:229	CTUn	226:229	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	9	53	theme	food	1282:1285	arg1	materials					1297:1305	bioactive bakery based food packaging materials	1259:1305	bioactive bakery based food packaging materials	1259:1305	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	8	54	theme	ductility	1155:1163	arg1	expense					1144:1150	the expense	1140:1150	the expense of ductility	1140:1163	Tensile tests showed CTUN sub-microparticles improved rigidity and tensile strength of the films at the expense of ductility.
31887941	5	55	theme	DSC	770:772	arg1	crystallization					753:767	MBi crystallization	749:767	MBi crystallization (DSC analysis)	749:782	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	5	55	theme	DSC	770:772	arg1	analysis					774:781	DSC analysis	770:781	DSC analysis	770:781	CTUn particles influenced MBi crystallization (DSC analysis) and promoted thermal degradation of MBi starch component (TGA).
31887941	9	56	theme	interesting	1208:1218	arg1	performances					1220:1231	interesting performances	1208:1231	interesting performances	1208:1231	Finally, MBi/CTUn biocomposites evidenced interesting performances potentially exploitablein bioactive bakery based food packaging materials.
31887941	4	57	from	values	715:720	arg1	films					652:656	films	652:656	films with different concentration of CTUn and at different pH values	652:720	In particular, the bioassays were performed on films with different concentration of CTUn and at different pH values.
31887941	0	58	theme	Antifungal	77:86	arg1	properties					109:118	Antifungal and chemico-physical properties	77:118	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.	0:132	Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films.
31887941	1	59	theme	bakery	324:329	arg1	products					331:338	bakery products	324:338	the bakery products deterioration	320:352	In this study, chitosan (C) tripolyphosphate (T) sub-micro particles containing ungeremine (CTUn), an alkaloid particularly active against Penicilliumroqueforti, a fungus responsible of the bakery products deterioration, were prepared through external gelation crosslinking process.
31887941	2	60	theme	starch	464:469	arg1	MBi					495:497	MBi	495:497	MBi	495:497	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
31887941	2	60	theme	starch	464:469	arg1	Mater-Bi					485:492	a thermoplastic starch based polymer Mater-Bi	448:492	a thermoplastic starch based polymer Mater-Bi (MBi)	448:498	The particles were included in a thermoplastic starch based polymer Mater-Bi (MBi), and MBi/CTUn bioactive biocomposites were obtained.
30321715	2	0	theme	pseudo-first-order	426:443	arg1	kinetics					445:452	the pseudo-first-order kinetics	422:452	the pseudo-first-order kinetics model	422:458	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	9	1	dep	had	1457:1459	arg1	made					1506:1509	made	1506:1509	made it have potential application on the industrial treatment of wastewater	1506:1581	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	1	2	theme	oxide	220:224	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	2	theme	oxide	220:224	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	8	3	theme	intermediate	1393:1404	arg1	products					1406:1413	the intermediate products	1389:1413	the intermediate products	1389:1413	Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products.
30321715	5	4	theme	possible	971:978	arg1	diagram					1014:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram was described.
30321715	3	5	theme	synergistic	774:784	arg1	effect					798:803	the synergistic enhancement effect	770:803	the synergistic enhancement effect among the components	770:824	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	8	6	theme	reaction	1330:1337	arg1	pathway					1339:1345	a probable reaction pathway	1319:1345	a probable reaction pathway of BPA removal	1319:1360	Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products.
30321715	2	7	theme	correlation	469:479	arg1	kobs					497:500	kobs	497:500	kobs	497:500	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	2	7	theme	correlation	469:479	arg1	constants					486:494	the correlation rate constants	465:494	the correlation rate constants (kobs)	465:501	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	6	8	theme	H2O2	1088:1091	arg1	effects					1041:1047	The effects	1037:1047	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency	1037:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	6	9	theme	catalyst	1070:1077	arg1	pH					1052:1053	pH	1052:1053	pH	1052:1053	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	6	9	theme	catalyst	1070:1077	arg1	concentrations					1103:1116	NH2OH concentrations	1097:1116	NH2OH concentrations	1097:1116	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	6	9	theme	catalyst	1070:1077	arg1	H2O2					1088:1091	initial H2O2	1080:1091	initial H2O2	1080:1091	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	6	9	theme	catalyst	1070:1077	arg1	dosage					1056:1061	dosage	1056:1061	dosage of the catalyst	1056:1077	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	3	10	theme	enhancement	786:796	arg1	effect					798:803	the synergistic enhancement effect	770:803	the synergistic enhancement effect among the components	770:824	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	1	11	theme	rGO	227:229	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	11	theme	rGO	227:229	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	0	12	theme	Synergistic	80:90	arg1	effect					92:97	Synergistic effect	80:97	Synergistic effect	80:97	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	12	theme	Synergistic	80:90	arg1	way					114:116	way	114:116	way	114:116	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	12	theme	Synergistic	80:90	arg1	principle					100:108	principle	100:108	principle	100:108	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	5	13	theme	three-element	980:992	arg1	diagram					1014:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram was described.
30321715	9	14	theme	industrial	1548:1557	arg1	treatment					1559:1567	the industrial treatment	1544:1567	the industrial treatment of wastewater	1544:1581	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	4	15	theme	•OH	905:907	arg1	generation					891:900	the generation	887:900	the generation of •OH	887:907	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	1	16	theme	synergistic	312:322	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	16	theme	synergistic	312:322	arg1	catalyzer					324:332	a synergistic catalyzer	310:332	a synergistic catalyzer for Bisphenol A (BPA) removal	310:362	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	3	17	theme	same	728:731	arg1	conditions					733:742	the same conditions	724:742	the same conditions	724:742	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	9	18	contain	had	1457:1459	arg1	composite					1447:1455	the composite	1443:1455	the composite	1443:1455	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	9	18	contain	had	1457:1459	arg2	performance					1487:1497	high and stable catalytic performance	1461:1497	high and stable catalytic performance	1461:1497	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	5	19	theme	schematic	1004:1012	arg1	diagram					1014:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram was described.
30321715	9	20	theme	catalytic	1477:1485	arg1	performance					1487:1497	high and stable catalytic performance	1461:1497	high and stable catalytic performance	1461:1497	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	6	21	theme	dosage	1056:1061	arg1	effects					1041:1047	The effects	1037:1047	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency	1037:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	0	22	theme	Fenton	14:19	arg1	degradation					21:31	Heterogeneous Fenton degradation	0:31	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.	0:132	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	9	23	theme	wastewater	1572:1581	arg1	treatment					1559:1567	the industrial treatment	1544:1567	the industrial treatment of wastewater	1544:1581	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	3	24	theme	catalytic	664:672	arg1	ability					674:680	stronger catalytic ability	655:680	stronger catalytic ability	655:680	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	5	25	theme	catalytic	994:1002	arg1	diagram					1014:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram	969:1020	A possible three-element catalytic schematic diagram was described.
30321715	1	26	theme	multi-component	151:165	arg1	β-cyclodextrin					177:190	β-cyclodextrin	177:190	β-cyclodextrin (β-CD)	177:197	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	26	theme	multi-component	151:165	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	26	theme	multi-component	151:165	arg1	catalyzer					324:332	a synergistic catalyzer	310:332	a synergistic catalyzer for Bisphenol A (BPA) removal	310:362	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	26	theme	multi-component	151:165	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	0	27	theme	Heterogeneous	0:12	arg1	degradation					21:31	Heterogeneous Fenton degradation	0:31	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.	0:132	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	1	28	theme	co-modified	232:242	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	28	theme	co-modified	232:242	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	6	29	theme	removal	1125:1131	arg1	efficiency					1133:1142	the removal efficiency	1121:1142	the removal efficiency	1121:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	6	30	theme	pH	1052:1053	arg1	effects					1041:1047	The effects	1037:1047	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency	1037:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	8	31	theme	probable	1321:1328	arg1	pathway					1339:1345	a probable reaction pathway	1319:1345	a probable reaction pathway of BPA removal	1319:1360	Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products.
30321715	3	32	theme	aqueous	701:707	arg1	solution					709:716	aqueous solution	701:716	aqueous solution	701:716	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	7	33	theme	excellent	1269:1277	arg1	stability					1279:1287	its excellent stability	1265:1287	its excellent stability	1265:1287	The removal of BPA and TOC retained 78.2 ± 2.4% and 52.9 ± 2.5% after five cycles, indicating its excellent stability and reusability.
30321715	6	34	from	effects	1041:1047	arg1	efficiency					1133:1142	the removal efficiency	1121:1142	the removal efficiency	1121:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	4	35	theme	β-CD	926:929	arg1	role					918:921	the role	914:921	the role of β-CD	914:929	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	1	36	theme	Bisphenol	338:346	arg1	BPA					351:353	BPA	351:353	BPA	351:353	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	36	theme	Bisphenol	338:346	arg1	A					348:348	Bisphenol A	338:348	Bisphenol A (BPA) removal	338:362	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	2	37	theme	BPA	409:411	arg1	reactions					396:404	catalytic reactions	386:404	catalytic reactions of BPA	386:411	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	7	38	theme	TOC	1194:1196	arg1	removal					1175:1181	The removal	1171:1181	The removal of BPA and TOC	1171:1196	The removal of BPA and TOC retained 78.2 ± 2.4% and 52.9 ± 2.5% after five cycles, indicating its excellent stability and reusability.
30321715	0	39	theme	A	46:46	arg1	degradation					21:31	Heterogeneous Fenton degradation	0:31	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.	0:132	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	3	40	theme	stronger	655:662	arg1	ability					674:680	stronger catalytic ability	655:680	stronger catalytic ability	655:680	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	0	41	theme	degradation	121:131	arg1	effect					92:97	Synergistic effect	80:97	Synergistic effect	80:97	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	41	theme	degradation	121:131	arg1	way					114:116	way	114:116	way	114:116	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	41	theme	degradation	121:131	arg1	principle					100:108	principle	100:108	principle	100:108	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	1	42	theme	A	348:348	arg1	removal					356:362	Bisphenol A (BPA) removal	338:362	Bisphenol A (BPA) removal	338:362	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	3	43	theme	Fe3O4	534:538	arg1	0.02173 min-1					546:558	0.02173 min-1	546:558	0.02173 min-1	546:558	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	3	43	theme	Fe3O4	534:538	arg1	β-CD					540:543	Fe3O4@β-CD	534:543	Fe3O4@β-CD (0.02173 min-1)	534:559	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	0	44	theme	bisphenol	36:44	arg1	A					46:46	bisphenol A	36:46	bisphenol A using Fe3O4@β-CD/rGO composite	36:77	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	2	45	theme	rate	481:484	arg1	kobs					497:500	kobs	497:500	kobs	497:500	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	2	45	theme	rate	481:484	arg1	constants					486:494	the correlation rate constants	465:494	the correlation rate constants (kobs)	465:501	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	3	46	theme	@	539:539	arg1	0.02173 min-1					546:558	0.02173 min-1	546:558	0.02173 min-1	546:558	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	3	46	theme	@	539:539	arg1	β-CD					540:543	Fe3O4@β-CD	534:543	Fe3O4@β-CD (0.02173 min-1)	534:559	Compared with Fe3O4@β-CD (0.02173 min-1), Fe3O4/rGO (0.09735 min-1) and Fe3O4 (0.01666 min-1), the composite (0.15733 min-1) exhibited stronger catalytic ability to remove BPA from aqueous solution under the same conditions, which were attributed to the synergistic enhancement effect among the components.
30321715	7	47	theme	BPA	1186:1188	arg1	removal					1175:1181	The removal	1171:1181	The removal of BPA and TOC	1171:1196	The removal of BPA and TOC retained 78.2 ± 2.4% and 52.9 ± 2.5% after five cycles, indicating its excellent stability and reusability.
30321715	0	48	theme	Fe3O4	54:58	arg1	composite					69:77	Fe3O4@β-CD/rGO composite	54:77	Fe3O4@β-CD/rGO composite	54:77	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	1	49	used	used	302:305	arg2	β-cyclodextrin					177:190	β-cyclodextrin	177:190	β-cyclodextrin (β-CD)	177:197	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	49	used	used	302:305	arg2	catalyzer					324:332	a synergistic catalyzer	310:332	a synergistic catalyzer for Bisphenol A (BPA) removal	310:362	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	49	used	used	302:305	arg2	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	49	used	used	302:305	arg2	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	4	50	from	introduction	831:842	arg1	composites					858:867	the composites	854:867	the composites	854:867	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	6	51	theme	concentrations	1103:1116	arg1	effects					1041:1047	The effects	1037:1047	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency	1037:1142	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	4	52	theme	rGO	847:849	arg1	beneficial					873:882	beneficial	873:882	beneficial	873:882	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	4	52	theme	rGO	847:849	arg1	introduction					831:842	The introduction	827:842	The introduction of rGO in the composites	827:867	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	1	53	theme	one-pot	270:276	arg1	method					291:296	one-pot solvothermal method	270:296	one-pot solvothermal method	270:296	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	8	54	theme	removal	1354:1360	arg1	pathway					1339:1345	a probable reaction pathway	1319:1345	a probable reaction pathway of BPA removal	1319:1360	Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products.
30321715	6	55	theme	NH2OH	1097:1101	arg1	concentrations					1103:1116	NH2OH concentrations	1097:1116	NH2OH concentrations	1097:1116	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	9	56	theme	high	1461:1464	arg1	performance					1487:1497	high and stable catalytic performance	1461:1497	high and stable catalytic performance	1461:1497	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	1	57	theme	solvothermal	278:289	arg1	method					291:296	one-pot solvothermal method	270:296	one-pot solvothermal method	270:296	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	8	58	theme	BPA	1350:1352	arg1	removal					1354:1360	BPA removal	1350:1360	BPA removal	1350:1360	Furthermore, a probable reaction pathway of BPA removal was suggested by analyzing the intermediate products.
30321715	9	59	theme	stable	1470:1475	arg1	performance					1487:1497	high and stable catalytic performance	1461:1497	high and stable catalytic performance	1461:1497	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	4	60	theme	•OH	964:966	arg1	utilization					949:959	the utilization	945:959	the utilization of •OH	945:966	The introduction of rGO in the composites was beneficial to the generation of •OH, and the role of β-CD might enhance the utilization of •OH.
30321715	2	61	dep	kinetics	445:452	arg1	model					454:458	model	454:458	the pseudo-first-order kinetics model	422:458	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	0	62	dep	degradation	21:31	arg1	effect					92:97	Synergistic effect	80:97	Synergistic effect	80:97	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	62	dep	degradation	21:31	arg1	way					114:116	way	114:116	way	114:116	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	62	dep	degradation	21:31	arg1	principle					100:108	principle	100:108	principle	100:108	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	0	63	theme	β-CD/rGO	60:67	arg1	composite					69:77	Fe3O4@β-CD/rGO composite	54:77	Fe3O4@β-CD/rGO composite	54:77	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	9	64	dep	it	1511:1512	arg1	have					1514:1517	have	1514:1517	have potential application on the industrial treatment of wastewater	1514:1581	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	6	65	theme	initial	1080:1086	arg1	H2O2					1088:1091	initial H2O2	1080:1091	initial H2O2	1080:1091	The effects of pH, dosage of the catalyst, initial H2O2 and NH2OH concentrations on the removal efficiency were further investigated.
30321715	1	66	theme	reduced	203:209	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	66	theme	reduced	203:209	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	0	67	theme	@	59:59	arg1	composite					69:77	Fe3O4@β-CD/rGO composite	54:77	Fe3O4@β-CD/rGO composite	54:77	Heterogeneous Fenton degradation of bisphenol A using Fe3O4@β-CD/rGO composite: Synergistic effect, principle and way of degradation.
30321715	9	68	theme	potential	1519:1527	arg1	application					1529:1539	potential application	1519:1539	potential application	1519:1539	All results indicated that the composite had high and stable catalytic performance, which made it have potential application on the industrial treatment of wastewater.
30321715	2	69	theme	catalytic	386:394	arg1	reactions					396:404	catalytic reactions	386:404	catalytic reactions of BPA	386:411	The study found that catalytic reactions of BPA followed the pseudo-first-order kinetics model, and the correlation rate constants (kobs) were calculated.
30321715	1	70	theme	graphene	211:218	arg1	catalyst					167:174	a multi-component catalyst	149:174	a multi-component catalyst	149:174	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
30321715	1	70	theme	graphene	211:218	arg1	Fe3O4					244:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	reduced graphene oxide (rGO) co-modified Fe3O4	203:248	In this study, a multi-component catalyst, β-cyclodextrin (β-CD) and reduced graphene oxide (rGO) co-modified Fe3O4, was fabricated via one-pot solvothermal method and used as a synergistic catalyzer for Bisphenol A (BPA) removal.
31227167	2	0	theme	tablet	502:507	arg1	porosity					509:516	tablet porosity	502:516	tablet porosity	502:516	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	7	1	theme	drug	1181:1184	arg1	properties					1194:1203	suitable physical and drug release properties	1159:1203	properties	1194:1203	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	6	2	theme	tuning	921:926	arg1	profiles					941:948	tuning drug release profiles	921:948	tuning drug release profiles through small changes in cellulose ethers nature and ratio	921:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	3	from	changes	964:970	arg1	nature					992:997	nature	992:997	nature	992:997	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	3	from	changes	964:970	arg1	ethers					985:990	cellulose ethers nature and ratio	975:1007	cellulose ethers nature and ratio	975:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	3	from	changes	964:970	arg1	ratio					1003:1007	ratio	1003:1007	ratio	1003:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	4	theme	small	958:962	arg1	changes					964:970	small changes	958:970	small changes in cellulose ethers nature and ratio	958:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	7	5	theme	release	1186:1192	arg1	properties					1194:1203	suitable physical and drug release properties	1159:1203	properties	1194:1203	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	6	theme	Semisolid	1010:1018	arg1	printing					1039:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	6	theme	Semisolid	1010:1018	arg1	approach					1074:1081	a feasible approach	1063:1081	a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery	1063:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	2	7	theme	release	351:357	arg1	formulations					359:370	Orodispersible (flash) and immediate release formulations	314:370	formulations	359:370	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	7	8	theme	extrusion-based	1020:1034	arg1	printing					1039:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	8	theme	extrusion-based	1020:1034	arg1	approach					1074:1081	a feasible approach	1063:1081	a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery	1063:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	6	9	theme	profiles	941:948	arg1	possibilities					904:916	the possibilities	900:916	the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio	900:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	9	theme	profiles	941:948	arg1	printlets					861:869	printlets	861:869	printlets of poorly soluble drugs	861:893	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	5	10	theme	Drug	698:701	arg1	crystallinity					703:715	Drug crystallinity	698:715	Drug crystallinity	698:715	Drug crystallinity, printlet disintegration and dissolution profiles were also characterized.
31227167	3	11	theme	printable	566:574	arg1	compositions					576:587	printable compositions	566:587	printable compositions	566:587	Rheology of the wet masses allowed identifying printable compositions.
31227167	1	12	theme	fine	274:277	arg1	adjustment					284:293	fine dose adjustment	274:293	fine dose adjustment	274:293	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	6	13	theme	release	933:939	arg1	profiles					941:948	tuning drug release profiles	921:948	tuning drug release profiles through small changes in cellulose ethers nature and ratio	921:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	3	14	theme	masses	539:544	arg1	Rheology					519:526	Rheology	519:526	Rheology of the wet masses	519:544	Rheology of the wet masses allowed identifying printable compositions.
31227167	1	15	theme	cyclodextrins	177:189	arg1	use					170:172	the use	166:172	the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release	166:311	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	6	16	theme	drug	928:931	arg1	profiles					941:948	tuning drug release profiles	921:948	tuning drug release profiles through small changes in cellulose ethers nature and ratio	921:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	2	17	theme	semisolid	392:400	arg1	extrusion					402:410	semisolid extrusion	392:410	semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers	392:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	0	18	theme	fast	35:38	arg1	carbamazepine					51:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	4	19	theme	Printing	590:597	arg1	robustness					599:608	Printing robustness	590:608	Printing robustness	590:608	Printing robustness was assessed evaluating weight, dimensions, hardness, drug content, and microstructure.
31227167	7	20	dep	approach	1074:1081	arg1	produce					1136:1142	produce	1136:1142	to produce printlets with suitable physical and drug release properties for oral delivery	1133:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	20	dep	approach	1074:1081	arg1	form					1094:1097	form	1094:1097	to in situ form carbamazepine-HPβCD complexes	1083:1127	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	0	21	theme	Hydroxypropyl-β-cyclodextrin-based	0:33	arg1	carbamazepine					51:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	1	22	theme	dose	279:282	arg1	adjustment					284:293	fine dose adjustment	274:293	fine dose adjustment	274:293	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	4	23	theme	drug	664:667	arg1	content					669:675	drug content	664:675	drug content	664:675	Printing robustness was assessed evaluating weight, dimensions, hardness, drug content, and microstructure.
31227167	2	24	theme	hydroxypropyl-β-cyclodextrin	429:456	arg1	masses					419:424	wet masses	415:424	wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers	415:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	5	25	theme	dissolution	746:756	arg1	profiles					758:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles were also characterized.
31227167	6	26	dep	ethers	985:990	arg1	nature					992:997	nature	992:997	nature	992:997	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	26	dep	ethers	985:990	arg1	ethers					985:990	cellulose ethers nature and ratio	975:1007	cellulose ethers nature and ratio	975:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	26	dep	ethers	985:990	arg1	ratio					1003:1007	ratio	1003:1007	ratio	1003:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	2	27	theme	masses	419:424	arg1	extrusion					402:410	semisolid extrusion	392:410	semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers	392:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	7	28	theme	oral	1209:1212	arg1	delivery					1214:1221	oral delivery	1209:1221	oral delivery	1209:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	1	29	theme	rapid	299:303	arg1	release					305:311	rapid release	299:311	rapid release	299:311	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	7	30	theme	carbamazepine-HPβCD	1099:1117	arg1	complexes					1119:1127	carbamazepine-HPβCD complexes	1099:1127	carbamazepine-HPβCD complexes	1099:1127	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	1	31	theme	soluble	222:228	arg1	carbamazepine					245:257	carbamazepine	245:257	carbamazepine	245:257	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	1	31	theme	soluble	222:228	arg1	drugs					230:234	poorly soluble drugs	215:234	poorly soluble drugs	215:234	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	2	32	theme	wet	415:417	arg1	masses					419:424	wet masses	415:424	wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers	415:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	2	33	theme	ethers	480:485	arg1	masses					419:424	wet masses	415:424	wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers	415:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	7	34	theme	feasible	1065:1072	arg1	printing					1039:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	34	theme	feasible	1065:1072	arg1	approach					1074:1081	a feasible approach	1063:1081	a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery	1063:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	1	35	theme	drugs	230:234	arg1	printlets					202:210	printlets	202:210	printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release	202:311	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	2	36	theme	cellulose	470:478	arg1	ethers					480:485	cellulose ethers	470:485	cellulose ethers	470:485	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	5	37	theme	crystallinity	703:715	arg1	profiles					758:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles were also characterized.
31227167	0	38	theme	extrusion	97:105	arg1	printing					110:117	semisolid extrusion 3D printing	87:117	semisolid extrusion 3D printing	87:117	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	2	39	theme	immediate	341:349	arg1	formulations					359:370	Orodispersible (flash) and immediate release formulations	314:370	formulations	359:370	Orodispersible (flash) and immediate release formulations were 3D printed via semisolid extrusion of wet masses of hydroxypropyl-β-cyclodextrin (HPβCD) and cellulose ethers and regulating tablet porosity.
31227167	0	40	dep	printlets	65:73	arg1	prepared					75:82	prepared	75:82	printlets prepared by semisolid extrusion 3D printing	65:117	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	7	41	with	printlets	1144:1152	arg1	properties					1194:1203	suitable physical and drug release properties	1159:1203	properties	1194:1203	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	41	with	printlets	1144:1152	arg1	physical					1168:1175	physical	1168:1175	physical	1168:1175	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	6	42	theme	cellulose	975:983	arg1	nature					992:997	nature	992:997	nature	992:997	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	42	theme	cellulose	975:983	arg1	ethers					985:990	cellulose ethers nature and ratio	975:1007	cellulose ethers nature and ratio	975:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	42	theme	cellulose	975:983	arg1	ratio					1003:1007	ratio	1003:1007	ratio	1003:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	5	43	theme	printlet	718:725	arg1	disintegration					727:740	printlet disintegration	718:740	printlet disintegration	718:740	Drug crystallinity, printlet disintegration and dissolution profiles were also characterized.
31227167	6	44	theme	drugs	889:893	arg1	possibilities					904:916	the possibilities	900:916	the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio	900:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	44	theme	drugs	889:893	arg1	printlets					861:869	printlets	861:869	printlets of poorly soluble drugs	861:893	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	5	45	theme	disintegration	727:740	arg1	profiles					758:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles	698:765	Drug crystallinity, printlet disintegration and dissolution profiles were also characterized.
31227167	6	46	theme	soluble	881:887	arg1	drugs					889:893	poorly soluble drugs	874:893	poorly soluble drugs	874:893	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	1	47	theme	first	155:159	arg1	time					161:164	the first time	151:164	the first time	151:164	This work aimed to explore for the first time the use of cyclodextrins to prepare printlets of poorly soluble drugs, such as carbamazepine, which require fine dose adjustment and rapid release.
31227167	0	48	theme	3D	107:108	arg1	printing					110:117	semisolid extrusion 3D printing	87:117	semisolid extrusion 3D printing	87:117	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	6	49	from	excipient	848:856	arg1	printlets					861:869	printlets	861:869	printlets of poorly soluble drugs	861:893	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	6	49	from	excipient	848:856	arg1	possibilities					904:916	the possibilities	900:916	the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio	900:1007	The results highlight the feasibility of using HPβCD as excipient in printlets of poorly soluble drugs, and the possibilities of tuning drug release profiles through small changes in cellulose ethers nature and ratio.
31227167	0	50	theme	semisolid	87:95	arg1	printing					110:117	semisolid extrusion 3D printing	87:117	semisolid extrusion 3D printing	87:117	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	0	51	theme	dissolving	40:49	arg1	carbamazepine					51:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine	0:63	Hydroxypropyl-β-cyclodextrin-based fast dissolving carbamazepine printlets prepared by semisolid extrusion 3D printing.
31227167	7	52	theme	3D	1036:1037	arg1	printing					1039:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing	1010:1046	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	7	52	theme	3D	1036:1037	arg1	approach					1074:1081	a feasible approach	1063:1081	a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery	1063:1221	Semisolid extrusion-based 3D printing is revealed as a feasible approach to in situ form carbamazepine-HPβCD complexes and to produce printlets with suitable physical and drug release properties for oral delivery.
31227167	3	53	theme	wet	535:537	arg1	masses					539:544	the wet masses	531:544	the wet masses	531:544	Rheology of the wet masses allowed identifying printable compositions.
30597396	3	0	from	effect	449:454	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	3	0	from	effect	449:454	arg1	properties					519:528	hydrolytic properties	508:528	hydrolytic properties	508:528	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	3	1	theme	hydrolytic	508:517	arg1	properties					519:528	hydrolytic properties	508:528	hydrolytic properties	508:528	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	1	2	theme	lignocellulosic	275:289	arg1	biomass					291:297	lignocellulosic biomass	275:297	lignocellulosic biomass	275:297	High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass.
30597396	6	3	theme	MALDI-TOF-MS	918:929	arg1	analysis					931:938	HPLC-UV and MALDI-TOF-MS analysis	906:938	HPLC-UV and MALDI-TOF-MS analysis	906:938	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	5	4	dep	%	833:833	arg1	to					835:836	to	835:836	to	835:836	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	2	5	theme	sugarcane	371:379	arg1	bagasse					381:387	sugarcane bagasse	371:387	sugarcane bagasse (SCB)	371:393	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	2	5	theme	sugarcane	371:379	arg1	SCB					390:392	SCB	390:392	SCB	390:392	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	4	6	theme	low	591:593	arg1	input					604:608	low chemical input	591:608	low chemical input	591:608	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	0	7	theme	functional	103:112	arg1	xylo-oligosaccharides					114:134	functional xylo-oligosaccharides	103:134	functional xylo-oligosaccharides	103:134	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	5	8	theme	Stepwise	673:680	arg1	hydrolysis					692:701	Stepwise enzymatic hydrolysis	673:701	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase	673:750	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	2	9	theme	enzymatic	315:323	arg1	hydrolysis					325:334	enzymatic hydrolysis	315:334	enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS	315:428	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	7	10	theme	industrial	1240:1249	arg1	application					1251:1261	industrial application	1240:1261	industrial application	1240:1261	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	6	11	theme	HPLC-UV	906:912	arg1	analysis					931:938	HPLC-UV and MALDI-TOF-MS analysis	906:938	HPLC-UV and MALDI-TOF-MS analysis	906:938	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	5	12	theme	enzymatic	682:690	arg1	hydrolysis					692:701	Stepwise enzymatic hydrolysis	673:701	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase	673:750	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	7	13	theme	fermentable	1192:1202	arg1	sugars					1204:1209	fermentable sugars	1192:1209	fermentable sugars	1192:1209	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	4	14	with	conditions	575:584	arg1	input					604:608	low chemical input	591:608	low chemical input	591:608	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	3	15	theme	chemical	482:489	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	7	16	from	SCB	1216:1218	arg1	co-production					1156:1168	the co-production	1152:1168	the co-production of functional XOS and fermentable sugars from SCB	1152:1218	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	7	16	from	SCB	1216:1218	arg1	XOS					1184:1186	functional XOS	1173:1186	functional XOS	1173:1186	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	7	16	from	SCB	1216:1218	arg1	sugars					1204:1209	fermentable sugars	1192:1209	fermentable sugars	1192:1209	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	5	17	theme	91.51	838:842	arg1	%					833:833	%	833:833	%	833:833	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	3	18	dep	compositions	491:502	arg1	the					478:480	the	478:480	the	478:480	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	5	19	with	hydrolysis	692:701	arg1	cellulase					742:750	cellulase	742:750	cellulase	742:750	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	5	19	with	hydrolysis	692:701	arg1	xylanase					729:736	xylanase	729:736	xylanase	729:736	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	6	20	theme	acid	1080:1083	arg1	acid					1080:1083	arabinose/4-O-methylglucuronic acid	1049:1083	arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1049:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	6	20	theme	acid	1080:1083	arg1	amount					1039:1044	a small amount	1031:1044	a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1031:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	0	21	theme	xylo-oligosaccharides	114:134	arg1	co-production					86:98	the co-production	82:98	the co-production of functional xylo-oligosaccharides and fermentable sugars	82:157	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	4	22	theme	chemical	595:602	arg1	input					604:608	low chemical input	591:608	low chemical input	591:608	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	7	23	theme	XOS	1184:1186	arg1	co-production					1156:1168	the co-production	1152:1168	the co-production of functional XOS and fermentable sugars from SCB	1152:1218	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	7	24	theme	application	1251:1261	arg1	potential					1227:1235	potential	1227:1235	potential of industrial application	1227:1261	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	1	25	theme	enzymatic	196:204	arg1	production					206:215	enzymatic production	196:215	enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass	196:297	High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass.
30597396	0	26	theme	enzymatic	9:17	arg1	hydrolysis					19:28	Stepwise enzymatic hydrolysis	0:28	Stepwise enzymatic hydrolysis of alkaline oxidation	0:50	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	2	27	theme	oxidation	348:356	arg1	hydrolysis					325:334	enzymatic hydrolysis	315:334	enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS	315:428	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	5	28	theme	enzymatic	868:876	arg1	hydrolysis					878:887	enzymatic hydrolysis	868:887	enzymatic hydrolysis using cellulase	868:903	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	0	29	theme	Stepwise	0:7	arg1	hydrolysis					19:28	Stepwise enzymatic hydrolysis	0:28	Stepwise enzymatic hydrolysis of alkaline oxidation	0:50	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	4	30	theme	pretreatment	562:573	arg1	conditions					575:584	The AO pretreatment conditions	555:584	The AO pretreatment conditions with low chemical input for the production of XOS	555:634	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	7	31	theme	proposed	1130:1137	arg1	strategy					1139:1146	The proposed strategy	1126:1146	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB	1126:1218	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	2	32	theme	alkaline	339:346	arg1	AO					359:360	AO	359:360	AO	359:360	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	2	32	theme	alkaline	339:346	arg1	oxidation					348:356	alkaline oxidation	339:356	alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS	339:428	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	7	33	theme	functional	1173:1182	arg1	XOS					1184:1186	functional XOS	1173:1186	functional XOS	1173:1186	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	5	34	theme	84.97	828:832	arg1	%					833:833	%	833:833	%	833:833	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	4	35	theme	AO	559:560	arg1	conditions					575:584	The AO pretreatment conditions	555:584	The AO pretreatment conditions with low chemical input for the production of XOS	555:634	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	0	36	theme	sugars	152:157	arg1	co-production					86:98	the co-production	82:98	the co-production of functional xylo-oligosaccharides and fermentable sugars	82:157	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	3	37	theme	pretreatment	462:473	arg1	effect					449:454	The effect	445:454	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB	445:535	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	0	38	theme	fermentable	140:150	arg1	sugars					152:157	fermentable sugars	140:157	fermentable sugars	140:157	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	2	39	theme	XOS	426:428	arg1	production					412:421	the production	408:421	the production of XOS	408:428	In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted.
30597396	0	40	theme	oxidation	42:50	arg1	hydrolysis					19:28	Stepwise enzymatic hydrolysis	0:28	Stepwise enzymatic hydrolysis of alkaline oxidation	0:50	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	6	41	theme	arabinose/4-O-methylglucuronic	1049:1078	arg1	acid					1080:1083	arabinose/4-O-methylglucuronic acid	1049:1083	arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1049:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	4	42	theme	orthogonal	654:663	arg1	design					665:670	orthogonal design	654:670	orthogonal design	654:670	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	0	43	theme	alkaline	33:40	arg1	oxidation					42:50	alkaline oxidation	33:50	alkaline oxidation	33:50	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	6	44	theme	XOS	968:970	arg1	products					972:979	the obtained XOS products	955:979	the obtained XOS products	955:979	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	7	45	theme	sugars	1204:1209	arg1	co-production					1156:1168	the co-production	1152:1168	the co-production of functional XOS and fermentable sugars from SCB	1152:1218	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
30597396	3	46	theme	AO	459:460	arg1	pretreatment					462:473	AO pretreatment	459:473	AO pretreatment	459:473	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	0	47	theme	sugarcane	60:68	arg1	bagasse					70:76	sugarcane bagasse	60:76	sugarcane bagasse	60:76	Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars.
30597396	6	48	theme	obtained	959:966	arg1	products					972:979	the obtained XOS products	955:979	the obtained XOS products	955:979	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	5	49	theme	SCB	720:722	arg1	hydrolysis					692:701	Stepwise enzymatic hydrolysis	673:701	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase	673:750	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	5	50	theme	AO	706:707	arg1	SCB					720:722	AO pretreated SCB	706:722	AO pretreated SCB	706:722	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	6	51	with	xylobiose	1005:1013	arg1	acid					1080:1083	arabinose/4-O-methylglucuronic acid	1049:1083	arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1049:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	6	51	with	xylobiose	1005:1013	arg1	amount					1039:1044	a small amount	1031:1044	a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1031:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	5	52	theme	pretreated	709:718	arg1	SCB					720:722	AO pretreated SCB	706:722	AO pretreated SCB	706:722	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	1	53	theme	High	160:163	arg1	input					174:178	High chemical input	160:178	High chemical input	160:178	High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass.
30597396	6	54	theme	small	1033:1037	arg1	acid					1080:1083	arabinose/4-O-methylglucuronic acid	1049:1083	arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1049:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	6	54	theme	small	1033:1037	arg1	amount					1039:1044	a small amount	1031:1044	a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1031:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	6	55	with	xylose	1019:1024	arg1	acid					1080:1083	arabinose/4-O-methylglucuronic acid	1049:1083	arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1049:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	6	55	with	xylose	1019:1024	arg1	amount					1039:1044	a small amount	1031:1044	a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose	1031:1123	HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose.
30597396	1	56	theme	xylo-oligosaccharides	220:240	arg1	production					206:215	enzymatic production	196:215	enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass	196:297	High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass.
30597396	1	57	theme	chemical	165:172	arg1	input					174:178	High chemical input	160:178	High chemical input	160:178	High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass.
30597396	4	58	theme	XOS	632:634	arg1	production					618:627	the production	614:627	the production of XOS	614:634	The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design.
30597396	5	59	theme	cellulose	792:800	arg1	conversion					802:811	the cellulose conversion	788:811	the cellulose conversion	788:811	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	5	59	theme	cellulose	792:800	arg1	meanwhile					777:785	meanwhile	777:785	meanwhile	777:785	Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase.
30597396	3	60	theme	SCB	533:535	arg1	compositions					491:502	chemical compositions	482:502	chemical compositions	482:502	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	3	60	theme	SCB	533:535	arg1	properties					519:528	hydrolytic properties	508:528	hydrolytic properties	508:528	The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated.
30597396	7	61	from	co-production	1156:1168	arg1	SCB					1216:1218	SCB	1216:1218	SCB	1216:1218	The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.
29293289	10	0	theme	liquid	1672:1677	arg1	chromatography					1679:1692	high-performance liquid chromatography	1655:1692	high-performance liquid chromatography	1655:1692	However, examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography were also found.
29293289	6	1	theme	enantiomers	954:964	arg1	majority					942:949	the majority	938:949	the majority of enantiomers	938:964	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	2	theme	/methanol/isopropylamine	975:998	arg1	v/v/v					1010:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	5	3	theme	co-solvents	790:800	arg1	influence					750:758	The influence	746:758	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives	746:859	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	10	4	theme	high-performance	1655:1670	arg1	chromatography					1679:1692	high-performance liquid chromatography	1655:1692	high-performance liquid chromatography	1655:1692	However, examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography were also found.
29293289	6	5	theme	phase	897:901	arg1	CO2					971:973	CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	971:1094	CO2	971:973	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	5	theme	phase	897:901	arg1	compositions					903:914	The best mobile phase compositions	881:914	The best mobile phase compositions for the separation of the majority of enantiomers	881:964	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	9	6	theme	Supercritical	1400:1412	arg1	chromatography					1420:1433	Supercritical fluid chromatography	1400:1433	Supercritical fluid chromatography	1400:1433	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	0	7	theme	drugs	104:108	arg1	enantioseparation					83:99	the enantioseparation	79:99	the enantioseparation of drugs in supercritical fluid chromatography	79:146	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	6	8	theme	80:20:0.1	1000:1008	arg1	v/v/v					1010:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	8	9	theme	liquid	1183:1188	arg1	chromatography					1190:1203	A high-performance liquid chromatography	1164:1203	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1164:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	6	10	theme	best	885:888	arg1	CO2					971:973	CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	971:1094	CO2	971:973	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	10	theme	best	885:888	arg1	compositions					903:914	The best mobile phase compositions	881:914	The best mobile phase compositions for the separation of the majority of enantiomers	881:964	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	9	11	theme	better	1475:1480	arg1	enantioresolution					1482:1498	better enantioresolution	1475:1498	better enantioresolution for shorter analysis times than high-performance liquid chromatography	1475:1569	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	4	12	theme	different	533:541	arg1	additives					543:551	Four different additives	528:551	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid)	528:623	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	12	theme	different	533:541	arg1	isopropylamine					583:596	isopropylamine	583:596	isopropylamine	583:596	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	12	theme	different	533:541	arg1	triethylamine					568:580	triethylamine	568:580	triethylamine	568:580	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	12	theme	different	533:541	arg1	acid					619:622	trifluoroacetic acid	603:622	trifluoroacetic acid	603:622	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	12	theme	different	533:541	arg1	diethylamine					554:565	diethylamine	554:565	diethylamine	554:565	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	8	13	theme	high-performance	1166:1181	arg1	chromatography					1190:1203	A high-performance liquid chromatography	1164:1203	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1164:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	6	14	theme	acid	1067:1070	arg1	v/v/v/v					1088:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	4	15	theme	trifluoroacetic	658:672	arg1	acid					674:677	trifluoroacetic acid	658:677	trifluoroacetic acid	658:677	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	0	16	theme	supercritical	113:125	arg1	chromatography					133:146	supercritical fluid chromatography	113:146	supercritical fluid chromatography	113:146	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	1	17	dep	different	335:343	arg1	physicochemical					364:378	physicochemical	364:378	physicochemical	364:378	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	1	17	dep	different	335:343	arg1	pharmaceutical					345:358	pharmaceutical	345:358	pharmaceutical	345:358	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	8	18	theme	separation	1205:1214	arg1	system					1216:1221	A high-performance liquid chromatography separation system	1164:1221	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1164:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	1	19	with	analytes	321:328	arg1	properties					380:389	different pharmaceutical and physicochemical properties	335:389	different pharmaceutical and physicochemical properties	335:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	9	20	theme	better	1454:1459	arg1	results					1461:1467	better results	1454:1467	better results	1454:1467	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	2	21	theme	fluid	450:454	arg1	chromatography					456:469	supercritical fluid chromatography	436:469	supercritical fluid chromatography	436:469	The enantioseparations were performed using supercritical fluid chromatography.
29293289	9	22	theme	shorter	1504:1510	arg1	times					1521:1525	shorter analysis times	1504:1525	shorter analysis times than high-performance liquid chromatography	1504:1569	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	8	23	theme	chromatography	1190:1203	arg1	system					1216:1221	A high-performance liquid chromatography separation system	1164:1221	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1164:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	10	24	theme	optimized	1631:1639	arg1	conditions					1641:1650	the optimized conditions	1627:1650	the optimized conditions in high-performance liquid chromatography	1627:1692	However, examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography were also found.
29293289	2	25	theme	supercritical	436:448	arg1	chromatography					456:469	supercritical fluid chromatography	436:469	supercritical fluid chromatography	436:469	The enantioseparations were performed using supercritical fluid chromatography.
29293289	8	26	theme	80:20:0.1	1353:1361	arg1	v/v/v					1363:1367	hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1325:1367	hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1325:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	5	27	dep	co-solvents	790:800	arg1	co-solvents					790:800	two different mobile phase co-solvents	763:800	two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives	763:859	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	27	dep	co-solvents	790:800	arg1	propan-2-ol					816:826	propan-2-ol	816:826	propan-2-ol	816:826	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	27	dep	co-solvents	790:800	arg1	methanol					803:810	methanol	803:810	methanol	803:810	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	8	28	theme	hexane/propan-2-ol/additive	1325:1351	arg1	v/v/v					1363:1367	hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1325:1367	hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1325:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	1	29	theme	enantioselective	278:293	arg1	separation					295:304	the enantioselective separation	274:304	the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties	274:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	6	30	theme	/propan-2-ol/isopropylamine/trifluoroacetic	1023:1065	arg1	v/v/v/v					1088:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	31	theme	majority	942:949	arg1	separation					924:933	the separation	920:933	the separation of the majority of enantiomers	920:964	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	4	32	dep	additives	543:551	arg1	additives					543:551	Four different additives	528:551	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid)	528:623	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	32	dep	additives	543:551	arg1	isopropylamine					583:596	isopropylamine	583:596	isopropylamine	583:596	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	32	dep	additives	543:551	arg1	triethylamine					568:580	triethylamine	568:580	triethylamine	568:580	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	32	dep	additives	543:551	arg1	acid					619:622	trifluoroacetic acid	603:622	trifluoroacetic acid	603:622	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	32	dep	additives	543:551	arg1	diethylamine					554:565	diethylamine	554:565	diethylamine	554:565	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	5	33	theme	phase	784:788	arg1	co-solvents					790:800	two different mobile phase co-solvents	763:800	two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives	763:859	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	33	theme	phase	784:788	arg1	propan-2-ol					816:826	propan-2-ol	816:826	propan-2-ol	816:826	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	33	theme	phase	784:788	arg1	methanol					803:810	methanol	803:810	methanol	803:810	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	7	34	theme	best	1101:1104	arg1	results					1106:1112	The best results	1097:1112	The best results	1097:1112	The best results were obtained from the group of basic β-blockers.
29293289	9	35	theme	fluid	1414:1418	arg1	chromatography					1420:1433	Supercritical fluid chromatography	1400:1433	Supercritical fluid chromatography	1400:1433	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	8	36	theme	v/v/v	1363:1367	arg1	mixture					1314:1320	a mixture	1312:1320	a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1312:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	9	37	theme	high-performance	1532:1547	arg1	chromatography					1556:1569	high-performance liquid chromatography	1532:1569	high-performance liquid chromatography	1532:1569	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	0	38	theme	fluid	127:131	arg1	chromatography					133:146	supercritical fluid chromatography	113:146	supercritical fluid chromatography	113:146	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	0	39	from	enantioseparation	83:99	arg1	chromatography					133:146	supercritical fluid chromatography	113:146	supercritical fluid chromatography	113:146	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	0	40	dep	phase	69:73	arg1	comparison					149:158	comparison	149:158	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.	0:169	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	1	41	theme	-based	217:222	arg1	tool					265:268	a tool	263:268	a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties	263:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	1	41	theme	-based	217:222	arg1	phase					242:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	0	42	theme	-based	44:49	arg1	phase					69:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	0:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.	0:169	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	9	43	theme	liquid	1549:1554	arg1	chromatography					1556:1569	high-performance liquid chromatography	1532:1569	high-performance liquid chromatography	1532:1569	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	8	44	theme	stationary	1244:1253	arg1	phase					1255:1259	same stationary phase	1239:1259	same stationary phase	1239:1259	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	1	45	theme	chiral	224:229	arg1	tool					265:268	a tool	263:268	a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties	263:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	1	45	theme	chiral	224:229	arg1	phase					242:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	4	46	from	influence	701:709	arg1	enantioseparation					714:730	enantioseparation	714:730	enantioseparation	714:730	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	3	47	theme	composition	503:513	arg1	effect					476:481	The effect	472:481	The effect of the mobile phase composition	472:513	The effect of the mobile phase composition was studied.
29293289	3	48	theme	mobile	490:495	arg1	composition					503:513	the mobile phase composition	486:513	the mobile phase composition	486:513	The effect of the mobile phase composition was studied.
29293289	1	49	theme	selected	312:319	arg1	analytes					321:328	21 selected analytes	309:328	21 selected analytes with different pharmaceutical and physicochemical properties	309:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	8	50	theme	same	1239:1242	arg1	phase					1255:1259	same stationary phase	1239:1259	same stationary phase	1239:1259	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	8	51	theme	properties	1289:1298	arg1	phase					1255:1259	same stationary phase	1239:1259	same stationary phase	1239:1259	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	8	51	theme	properties	1289:1298	arg1	phase					1272:1276	mobile phase	1265:1276	mobile phase	1265:1276	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	7	52	theme	basic	1146:1150	arg1	β-blockers					1152:1161	basic β-blockers	1146:1161	basic β-blockers	1146:1161	The best results were obtained from the group of basic β-blockers.
29293289	1	53	theme	stationary	231:240	arg1	tool					265:268	a tool	263:268	a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties	263:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	1	53	theme	stationary	231:240	arg1	phase					242:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	171:246	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	0	54	theme	stationary	58:67	arg1	phase					69:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	0:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.	0:169	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	1	55	theme	analytes	321:328	arg1	separation					295:304	the enantioselective separation	274:304	the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties	274:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	10	56	theme	enantiomers	1593:1603	arg1	examples					1581:1588	examples	1581:1588	examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography	1581:1692	However, examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography were also found.
29293289	7	57	theme	β-blockers	1152:1161	arg1	group					1137:1141	the group	1133:1141	the group of basic β-blockers	1133:1161	The best results were obtained from the group of basic β-blockers.
29293289	7	57	theme	β-blockers	1152:1161	arg1	β-blockers					1152:1161	basic β-blockers	1146:1161	basic β-blockers	1146:1161	The best results were obtained from the group of basic β-blockers.
29293289	0	58	theme	chiral	51:56	arg1	phase					69:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase	0:73	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.	0:169	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	4	59	theme	trifluoroacetic	603:617	arg1	additives					543:551	Four different additives	528:551	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid)	528:623	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	4	59	theme	trifluoroacetic	603:617	arg1	acid					619:622	trifluoroacetic acid	603:622	trifluoroacetic acid	603:622	Four different additives (diethylamine, triethylamine, isopropylamine, and trifluoroacetic acid) and isopropylamine combined with trifluoroacetic acid were tested and their influence on enantioseparation was compared.
29293289	0	60	with	comparison	149:158	arg1	HPLC					165:168	HPLC	165:168	HPLC	165:168	Cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase for the enantioseparation of drugs in supercritical fluid chromatography: comparison with HPLC.
29293289	1	61	theme	different	335:343	arg1	properties					380:389	different pharmaceutical and physicochemical properties	335:389	different pharmaceutical and physicochemical properties	335:389	A cellulose tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase was studied as a tool for the enantioselective separation of 21 selected analytes with different pharmaceutical and physicochemical properties.
29293289	5	62	theme	different	767:775	arg1	co-solvents					790:800	two different mobile phase co-solvents	763:800	two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives	763:859	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	62	theme	different	767:775	arg1	propan-2-ol					816:826	propan-2-ol	816:826	propan-2-ol	816:826	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	62	theme	different	767:775	arg1	methanol					803:810	methanol	803:810	methanol	803:810	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	6	63	theme	mobile	890:895	arg1	CO2					971:973	CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	971:1094	CO2	971:973	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	63	theme	mobile	890:895	arg1	compositions					903:914	The best mobile phase compositions	881:914	The best mobile phase compositions for the separation of the majority of enantiomers	881:964	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	10	64	from	conditions	1641:1650	arg1	chromatography					1679:1692	high-performance liquid chromatography	1655:1692	high-performance liquid chromatography	1655:1692	However, examples of enantiomers better resolved under the optimized conditions in high-performance liquid chromatography were also found.
29293289	5	65	theme	mobile	777:782	arg1	co-solvents					790:800	two different mobile phase co-solvents	763:800	two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives	763:859	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	65	theme	mobile	777:782	arg1	propan-2-ol					816:826	propan-2-ol	816:826	propan-2-ol	816:826	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	5	65	theme	mobile	777:782	arg1	methanol					803:810	methanol	803:810	methanol	803:810	The influence of two different mobile phase co-solvents (methanol and propan-2-ol) combined with all the additives was also evaluated.
29293289	9	66	dep	enantioresolution	1482:1498	arg1	i.e.					1470:1473	i.e.	1470:1473	i.e.	1470:1473	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	8	67	theme	similar	1281:1287	arg1	properties					1289:1298	similar properties	1281:1298	similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v	1281:1367	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	9	68	theme	analysis	1512:1519	arg1	times					1521:1525	shorter analysis times	1504:1525	shorter analysis times than high-performance liquid chromatography	1504:1569	Supercritical fluid chromatography was found to yield better results, i.e. better enantioresolution for shorter analysis times than high-performance liquid chromatography.
29293289	3	69	theme	phase	497:501	arg1	composition					503:513	the mobile phase composition	486:513	the mobile phase composition	486:513	The effect of the mobile phase composition was studied.
29293289	6	70	dep	CO2	971:973	arg1	v/v/v					1010:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	/methanol/isopropylamine 80:20:0.1 v/v/v	975:1014	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	6	70	dep	CO2	971:973	arg1	v/v/v/v					1088:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29293289	8	71	dep	phase	1255:1259	arg1	the					1235:1237	the	1235:1237	the	1235:1237	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	8	72	theme	mobile	1265:1270	arg1	phase					1272:1276	mobile phase	1265:1276	mobile phase	1265:1276	A high-performance liquid chromatography separation system composed of the same stationary phase and mobile phase of similar properties prepared as a mixture of hexane/propan-2-ol/additive 80:20:0.1 v/v/v was considered for comparison.
29293289	6	73	theme	80:20:0.05:0.05	1072:1086	arg1	v/v/v/v					1088:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	/propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v	1023:1094	The best mobile phase compositions for the separation of the majority of enantiomers were CO2 /methanol/isopropylamine 80:20:0.1 v/v/v or CO2 /propan-2-ol/isopropylamine/trifluoroacetic acid 80:20:0.05:0.05 v/v/v/v.
29425459	1	0	theme	water/ethanol/acetic	171:190	arg1	solution					197:204	water/ethanol/acetic acid solution	171:204	water/ethanol/acetic acid solution	171:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	1	1	theme	acid	192:195	arg1	solution					197:204	water/ethanol/acetic acid solution	171:204	water/ethanol/acetic acid solution	171:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	5	2	theme	gelatin	827:833	arg1	ratio					835:839	higher gelatin ratio	820:839	higher gelatin ratio (75%)	820:845	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	2	theme	gelatin	827:833	arg1	%					844:844	75%	842:844	75%	842:844	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	6	3	theme	bioactive	1249:1257	arg1	encapsulation					1259:1271	bioactive encapsulation	1249:1271	bioactive encapsulation	1249:1271	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	6	4	theme	food	1274:1277	arg1	packaging					1279:1287	food packaging	1274:1287	food packaging	1274:1287	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	2	5	theme	solubility	338:347	arg1	parameters					349:358	Hansen solubility parameters	331:358	Hansen solubility parameters	331:358	The solution compatibility was predicted based on Hansen solubility parameters and evaluated by rheological measurements.
29425459	4	6	theme	hydrogen	701:708	arg1	bonds					710:714	hydrogen bonds	701:714	hydrogen bonds	701:714	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	6	7	theme	physical	1196:1203	arg1	properties					1205:1214	tunable physical properties	1188:1214	tunable physical properties	1188:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	5	8	with	fibers	808:813	arg1	ratio					835:839	higher gelatin ratio	820:839	higher gelatin ratio (75%)	820:845	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	8	with	fibers	808:813	arg1	%					844:844	75%	842:844	75%	842:844	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	1	9	from	ratios	161:166	arg1	electrospun					211:221	electrospun	211:221	electrospun	211:221	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	1	9	from	ratios	161:166	arg1	blends					136:141	the ethylcellulose/gelatin blends	109:141	the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution	109:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	2	10	theme	Hansen	331:336	arg1	parameters					349:358	Hansen solubility parameters	331:358	Hansen solubility parameters	331:358	The solution compatibility was predicted based on Hansen solubility parameters and evaluated by rheological measurements.
29425459	1	11	theme	weight	154:159	arg1	ratios					161:166	various weight ratios	146:166	various weight ratios	146:166	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	3	12	theme	physical	407:414	arg1	properties					416:425	The physical properties	403:425	The physical properties	403:425	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	4	13	dep	ethylcellulose	659:672	arg1	chains					686:691	chains	686:691	chains	686:691	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	5	14	with	fibers	966:971	arg1	proportion					1000:1009	higher ethylcellulose proportion	978:1009	higher ethylcellulose proportion (75%)	978:1015	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	14	with	fibers	966:971	arg1	%					1014:1014	75%	1012:1014	75%	1012:1014	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	15	theme	contact	885:891	arg1	angle					893:897	water contact angle	879:897	water contact angle of 53.5°	879:906	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	4	16	theme	composite	754:762	arg1	fibers					764:769	the composite fibers	750:769	the composite fibers with improved thermal stability	750:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	5	17	theme	water	879:883	arg1	angle					893:897	water contact angle	879:897	water contact angle of 53.5°	879:906	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	18	theme	hydrophilic	858:868	arg1	surface					870:876	hydrophilic surface	858:876	hydrophilic surface (water contact angle of 53.5°)	858:907	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	19	theme	uptake	929:934	arg1	ability					936:942	adequate water uptake ability	914:942	adequate water uptake ability (1234.14%)	914:953	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	19	theme	uptake	929:934	arg1	%					952:952	1234.14%	945:952	1234.14%	945:952	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	0	20	theme	Physical	8:15	arg1	Properties					17:26	Tunable Physical Properties	0:26	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.	0:93	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.
29425459	6	21	theme	filtration	1294:1303	arg1	applications					1305:1316	filtration applications	1294:1316	filtration applications	1294:1316	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	5	22	theme	higher	978:983	arg1	proportion					1000:1009	higher ethylcellulose proportion	978:1009	higher ethylcellulose proportion (75%)	978:1015	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	22	theme	higher	978:983	arg1	%					1014:1014	75%	1012:1014	75%	1012:1014	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	3	23	theme	scanning	502:509	arg1	calorimetry					511:521	differential scanning calorimetry	489:521	differential scanning calorimetry	489:521	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	0	24	theme	Tunable	0:6	arg1	Properties					17:26	Tunable Physical Properties	0:26	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.	0:93	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.
29425459	5	25	dep	surface	870:876	arg1	angle					893:897	water contact angle	879:897	water contact angle of 53.5°	879:906	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	1	26	from	blends	136:141	arg1	solution					197:204	water/ethanol/acetic acid solution	171:204	water/ethanol/acetic acid solution	171:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	5	27	theme	ethylcellulose	985:998	arg1	proportion					1000:1009	higher ethylcellulose proportion	978:1009	higher ethylcellulose proportion (75%)	978:1015	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	27	theme	ethylcellulose	985:998	arg1	%					1014:1014	75%	1012:1014	75%	1012:1014	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	4	28	theme	fibers	764:769	arg1	morphology					736:745	a fine morphology	729:745	a fine morphology of the composite fibers with improved thermal stability	729:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	4	29	theme	fine	731:734	arg1	morphology					736:745	a fine morphology	729:745	a fine morphology of the composite fibers with improved thermal stability	729:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	3	30	theme	scanning	449:456	arg1	microscopy					467:476	scanning electron microscopy	449:476	scanning electron microscopy	449:476	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	1	31	theme	ethylcellulose/gelatin	113:134	arg1	electrospun					211:221	electrospun	211:221	electrospun	211:221	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	1	31	theme	ethylcellulose/gelatin	113:134	arg1	blends					136:141	the ethylcellulose/gelatin blends	109:141	the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution	109:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	6	32	theme	tunable	1188:1194	arg1	properties					1205:1214	tunable physical properties	1188:1214	tunable physical properties	1188:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	0	33	theme	Composite	54:62	arg1	Nanofibers					64:73	Ethylcellulose/Gelatin Composite Nanofibers	31:73	Ethylcellulose/Gelatin Composite Nanofibers	31:73	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.
29425459	6	34	theme	composite	1139:1147	arg1	nanofibers					1172:1181	the composite ethylcellulose/gelatin nanofibers	1135:1181	the composite ethylcellulose/gelatin nanofibers with tunable physical properties	1135:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	4	35	with	fibers	764:769	arg1	stability					793:801	improved thermal stability	776:801	improved thermal stability	776:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	5	36	theme	higher	820:825	arg1	ratio					835:839	higher gelatin ratio	820:839	higher gelatin ratio (75%)	820:845	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	36	theme	higher	820:825	arg1	%					844:844	75%	842:844	75%	842:844	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	0	37	theme	Ethylcellulose/Gelatin	31:52	arg1	Nanofibers					64:73	Ethylcellulose/Gelatin Composite Nanofibers	31:73	Ethylcellulose/Gelatin Composite Nanofibers	31:73	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.
29425459	1	38	with	nanofibers	236:245	arg1	properties					269:278	tunable physical properties	252:278	tunable physical properties	252:278	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	5	39	theme	129.7°	1101:1106	arg1	angle					1092:1096	water contact angle	1078:1096	water contact angle of 129.7°	1078:1106	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	40	theme	adequate	914:921	arg1	uptake					929:934	adequate water uptake	914:934	adequate water uptake ability (1234.14%)	914:953	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	1	41	theme	tunable	252:258	arg1	properties					269:278	tunable physical properties	252:278	tunable physical properties	252:278	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	3	42	theme	contact	593:599	arg1	angle					601:605	water contact angle	587:605	water contact angle	587:605	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	5	43	theme	53.5°	902:906	arg1	angle					893:897	water contact angle	879:897	water contact angle of 53.5°	879:906	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	6	44	theme	ethylcellulose/gelatin	1149:1170	arg1	nanofibers					1172:1181	the composite ethylcellulose/gelatin nanofibers	1135:1181	the composite ethylcellulose/gelatin nanofibers with tunable physical properties	1135:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	5	45	theme	water	923:927	arg1	uptake					929:934	adequate water uptake	914:934	adequate water uptake ability (1234.14%)	914:953	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	0	46	theme	Nanofibers	64:73	arg1	Properties					17:26	Tunable Physical Properties	0:26	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.	0:93	Tunable Physical Properties of Ethylcellulose/Gelatin Composite Nanofibers by Electrospinning.
29425459	4	47	theme	entangled	632:640	arg1	structures					642:651	the entangled structures	628:651	the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds	628:714	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	5	48	contain	possessed	848:856	arg1	fibers					808:813	The fibers	804:813	The fibers	804:813	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	48	contain	possessed	848:856	arg2	ability					936:942	adequate water uptake ability	914:942	adequate water uptake ability (1234.14%)	914:953	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	48	contain	possessed	848:856	arg2	%					952:952	1234.14%	945:952	1234.14%	945:952	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	48	contain	possessed	848:856	arg2	surface					870:876	hydrophilic surface	858:876	hydrophilic surface (water contact angle of 53.5°)	858:907	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	5	49	theme	hydrophobic	1057:1067	arg1	surface					1069:1075	a hydrophobic surface	1055:1075	a hydrophobic surface (water contact angle of 129.7°)	1055:1107	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	3	50	theme	water	587:591	arg1	angle					601:605	water contact angle	587:605	water contact angle	587:605	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	5	51	with	stable	1043:1048	arg1	surface					1069:1075	a hydrophobic surface	1055:1075	a hydrophobic surface (water contact angle of 129.7°)	1055:1107	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	4	52	theme	improved	776:783	arg1	stability					793:801	improved thermal stability	776:801	improved thermal stability	776:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	1	53	theme	various	146:152	arg1	ratios					161:166	various weight ratios	146:166	various weight ratios	146:166	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	1	54	theme	physical	260:267	arg1	properties					269:278	tunable physical properties	252:278	tunable physical properties	252:278	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	5	55	theme	water	1078:1082	arg1	angle					1092:1096	water contact angle	1078:1096	water contact angle of 129.7°	1078:1106	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	3	56	dep	Fourier	542:548	arg1	transform					550:558	transform	550:558	transform infrared spectroscopy	550:580	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	1	57	from	electrospun	211:221	arg1	work					103:106	this work	98:106	this work	98:106	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	2	58	theme	rheological	377:387	arg1	measurements					389:400	rheological measurements	377:400	rheological measurements	377:400	The solution compatibility was predicted based on Hansen solubility parameters and evaluated by rheological measurements.
29425459	1	59	from	work	103:106	arg1	electrospun					211:221	electrospun	211:221	electrospun	211:221	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	1	59	from	work	103:106	arg1	blends					136:141	the ethylcellulose/gelatin blends	109:141	the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution	109:204	In this work, the ethylcellulose/gelatin blends at various weight ratios in water/ethanol/acetic acid solution were electrospun to fabricate nanofibers with tunable physical properties.
29425459	6	60	contain	have	1216:1219	arg2	materials					1235:1243	materials	1235:1243	materials for bioactive encapsulation, food packaging, and filtration applications	1235:1316	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	6	60	contain	have	1216:1219	arg1	nanofibers					1172:1181	the composite ethylcellulose/gelatin nanofibers	1135:1181	the composite ethylcellulose/gelatin nanofibers with tunable physical properties	1135:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	6	60	contain	have	1216:1219	arg2	potentials					1221:1230	potentials	1221:1230	potentials	1221:1230	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29425459	5	61	theme	contact	1084:1090	arg1	angle					1092:1096	water contact angle	1078:1096	water contact angle of 129.7°	1078:1106	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	3	62	dep	transform	550:558	arg1	infrared					560:567	infrared	560:567	transform infrared spectroscopy	550:580	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	4	63	theme	thermal	785:791	arg1	stability					793:801	improved thermal stability	776:801	improved thermal stability	776:801	Results showed that the entangled structures among ethylcellulose and gelatin chains through hydrogen bonds gave rise to a fine morphology of the composite fibers with improved thermal stability.
29425459	5	64	dep	surface	1069:1075	arg1	angle					1092:1096	water contact angle	1078:1096	water contact angle of 129.7°	1078:1106	The fibers with higher gelatin ratio (75%), possessed hydrophilic surface (water contact angle of 53.5°), and adequate water uptake ability (1234.14%), while the fibers with higher ethylcellulose proportion (75%) tended to be highly water stable with a hydrophobic surface (water contact angle of 129.7°).
29425459	3	65	theme	differential	489:500	arg1	calorimetry					511:521	differential scanning calorimetry	489:521	differential scanning calorimetry	489:521	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	2	66	theme	solution	285:292	arg1	compatibility					294:306	The solution compatibility	281:306	The solution compatibility	281:306	The solution compatibility was predicted based on Hansen solubility parameters and evaluated by rheological measurements.
29425459	3	67	theme	electron	458:465	arg1	microscopy					467:476	scanning electron microscopy	449:476	scanning electron microscopy	449:476	The physical properties were characterized by scanning electron microscopy, porosity, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and water contact angle.
29425459	6	68	with	nanofibers	1172:1181	arg1	properties					1205:1214	tunable physical properties	1188:1214	tunable physical properties	1188:1214	This work suggested that the composite ethylcellulose/gelatin nanofibers with tunable physical properties have potentials as materials for bioactive encapsulation, food packaging, and filtration applications.
29388544	5	0	theme	activities	652:661	arg1	result					632:637	a result	630:637	a result of enzymatic activities	630:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	0	theme	activities	652:661	arg1	liquefaction					554:565	Complete liquefaction	545:565	Complete liquefaction of agar and κ-carrageenan	545:591	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	2	1	theme	bacterial	109:117	arg1	A3T					127:129	A3T	127:129	A3T	127:129	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	2	1	theme	bacterial	109:117	arg1	strain					119:124	A novel bacterial strain	101:124	A novel bacterial strain	101:124	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	12	2	theme	echini	1724:1729	arg1	sp					1731:1732	the name Colwellia echini sp	1705:1732	the name Colwellia echini sp	1705:1732	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	3	3	theme	optimum	423:429	arg1	NaCl					417:420	1-6 % (w/v) NaCl	405:420	1-6 % (w/v) NaCl (optimum 3 %)	405:434	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	3	3	theme	optimum	423:429	arg1	%					433:433	optimum 3 %	423:433	optimum 3 %	423:433	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	7	4	theme	polar	837:841	arg1	lipids					843:848	polar lipids	837:848	polar lipids	837:848	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	2	5	theme	novel	103:107	arg1	A3T					127:129	A3T	127:129	A3T	127:129	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	2	5	theme	novel	103:107	arg1	strain					119:124	A novel bacterial strain	101:124	A novel bacterial strain	101:124	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	5	6	theme	Complete	545:552	arg1	result					632:637	a result	630:637	a result of enzymatic activities	630:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	6	theme	Complete	545:552	arg1	liquefaction					554:565	Complete liquefaction	545:565	Complete liquefaction of agar and κ-carrageenan	545:591	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	9	7	theme	16S	1002:1004	arg1	rRNA					1006:1009	16S rRNA	1002:1009	the 16S rRNA gene	998:1014	Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria.
29388544	3	8	theme	3 	431:432	arg1	NaCl					417:420	1-6 % (w/v) NaCl	405:420	1-6 % (w/v) NaCl (optimum 3 %)	405:434	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	3	8	theme	3 	431:432	arg1	%					433:433	optimum 3 %	423:433	optimum 3 %	423:433	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	12	9	theme	phenotypic	1559:1568	arg1	analyses					1596:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	9	10	theme	rRNA	1006:1009	arg1	gene					1011:1014	the 16S rRNA gene	998:1014	the 16S rRNA gene	998:1014	Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria.
29388544	14	11	theme	type	1757:1760	arg1	A3T					1772:1774	A3T	1772:1774	A3T (=LMG 30125T=NCIMB 15095T)	1772:1801	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	14	11	theme	type	1757:1760	arg1	strain					1762:1767	The type strain	1753:1767	The type strain	1753:1767	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	11	12	theme	other	1530:1534	arg1	species					1546:1552	other Colwellia species	1530:1552	other Colwellia species	1530:1552	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	7	13	theme	ubiquinones	795:805	arg1	Q-8					807:809	ubiquinones Q-8	795:809	ubiquinones Q-8 (92 %)	795:816	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	7	13	theme	ubiquinones	795:805	arg1	%					815:815	92 %	812:815	92 %	812:815	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	7	13	theme	ubiquinones	795:805	arg1	quinones					764:771	The respiratory quinones	748:771	The respiratory quinones	748:771	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	1	14	theme	carrageenan-solubilizing	40:63	arg1	bacterium					65:73	an agar- and carrageenan-solubilizing bacterium	27:73	an agar- and carrageenan-solubilizing bacterium	27:73	nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
29388544	1	14	theme	carrageenan-solubilizing	40:63	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
29388544	12	15	theme	strain	1633:1638	arg1	A3T					1640:1642	strain A3T	1633:1642	strain A3T	1633:1642	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	3	16	dep	pH	376:377	arg1	optimum					384:390	optimum	384:390	optimum at pH 7	384:398	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	11	17	theme	average	1329:1335	arg1	identity					1348:1355	The average nucleotide identity	1325:1355	The average nucleotide identity between strain A3T and other members of Colwellia	1325:1405	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	11	17	theme	average	1329:1335	arg1	%					1421:1421	78.6-80.5 %	1411:1421	78.6-80.5 %	1411:1421	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	5	18	located	observed	597:604	arg1	media					621:625	solid plate media	609:625	solid plate media	609:625	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	18	located	observed	597:604	arg2	liquefaction					554:565	Complete liquefaction	545:565	Complete liquefaction of agar and κ-carrageenan	545:591	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	18	located	observed	597:604	arg2	result					632:637	a result	630:637	a result of enzymatic activities	630:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	11	19	theme	strain	1365:1370	arg1	A3T					1372:1374	strain A3T	1365:1374	strain A3T	1365:1374	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	11	20	theme	nucleotide	1337:1346	arg1	identity					1348:1355	The average nucleotide identity	1325:1355	The average nucleotide identity between strain A3T and other members of Colwellia	1325:1405	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	11	20	theme	nucleotide	1337:1346	arg1	%					1421:1421	78.6-80.5 %	1411:1421	78.6-80.5 %	1411:1421	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	11	21	theme	other	1380:1384	arg1	members					1386:1392	other members	1380:1392	other members of Colwellia	1380:1405	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	12	22	theme	name	1709:1712	arg1	sp					1731:1732	the name Colwellia echini sp	1705:1732	the name Colwellia echini sp	1705:1732	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	14	23	theme	30125T=NCIMB	1782:1793	arg1	A3T					1772:1774	A3T	1772:1774	A3T (=LMG 30125T=NCIMB 15095T)	1772:1801	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	14	23	theme	30125T=NCIMB	1782:1793	arg1	15095T					1795:1800	=LMG 30125T=NCIMB 15095T	1777:1800	=LMG 30125T=NCIMB 15095T	1777:1800	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	11	24	theme	Colwellia	1397:1405	arg1	A3T					1372:1374	strain A3T	1365:1374	strain A3T	1365:1374	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	11	24	theme	Colwellia	1397:1405	arg1	members					1386:1392	other members	1380:1392	other members of Colwellia	1380:1405	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	0	25	theme	Colwellia	0:8	arg1	echini					10:15	Colwellia echini	0:15	Colwellia echini	0:15	Colwellia echini sp.
29388544	11	26	theme	Colwellia	1536:1544	arg1	species					1546:1552	other Colwellia species	1530:1552	other Colwellia species	1530:1552	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	6	27	theme	fatty	670:674	arg1	acids					676:680	Major fatty acids	664:680	Major fatty acids	664:680	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	1	28	theme	sea	89:91	arg1	urchin					93:98	sea urchin	89:98	sea urchin	89:98	nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
29388544	6	29	dep	feature	694:700	arg1	C16 					705:708	C16 	705:708	C16 	705:708	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	12	30	theme	Colwellia	1714:1722	arg1	sp					1731:1732	the name Colwellia echini sp	1705:1732	the name Colwellia echini sp	1705:1732	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	3	31	theme	optimum	360:366	arg1	5-25 °C					351:357	5-25 °C	351:357	5-25 °C (optimum 20 °C)	351:373	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	3	31	theme	optimum	360:366	arg1	20 °C					368:372	optimum 20 °C	360:372	optimum 20 °C	360:372	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	6	32	dep	C16 	705:708	arg1	 1ω7c					710:714	 1ω7c	710:714	 1ω7c	710:714	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	6	32	dep	C16 	705:708	arg1	 1ω6c					728:732	 1ω6c	728:732	 1ω6c	728:732	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	6	32	dep	C16 	705:708	arg1	C16 					723:726	C16 	723:726	C16 	723:726	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	11	33	theme	hybridization	1436:1448	arg1	prediction					1450:1459	DNA-DNA hybridization prediction	1428:1459	DNA-DNA hybridization prediction	1428:1459	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	6	34	theme	Major	664:668	arg1	acids					676:680	Major fatty acids	664:680	Major fatty acids	664:680	Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0.
29388544	12	35	theme	phylogenetic	1571:1582	arg1	analyses					1596:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	4	36	theme	sole	525:528	arg1	source					537:542	sole carbon source	525:542	sole carbon source	525:542	Furthermore, strain A3T grew on agar, agarose, κ-carrageenan, alginate and laminarin as sole carbon source.
29388544	3	37	theme	%	409:409	arg1	NaCl					417:420	1-6 % (w/v) NaCl	405:420	1-6 % (w/v) NaCl (optimum 3 %)	405:434	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	3	37	theme	%	409:409	arg1	%					433:433	optimum 3 %	423:433	optimum 3 %	423:433	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	9	38	theme	genus	1066:1070	arg1	Colwellia					1072:1080	the genus Colwellia	1062:1080	the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria	1062:1135	Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria.
29388544	10	39	theme	16S	1151:1153	arg1	similarity					1174:1183	16S rRNA gene sequence similarity	1151:1183	16S rRNA gene sequence similarity between strain A3T and its closest relatives	1151:1228	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	14	40	theme	=LMG	1777:1780	arg1	A3T					1772:1774	A3T	1772:1774	A3T (=LMG 30125T=NCIMB 15095T)	1772:1801	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	14	40	theme	=LMG	1777:1780	arg1	15095T					1795:1800	=LMG 30125T=NCIMB 15095T	1777:1800	=LMG 30125T=NCIMB 15095T	1777:1800	The type strain is A3T (=LMG 30125T=NCIMB 15095T).
29388544	5	41	theme	solid	609:613	arg1	media					621:625	solid plate media	609:625	solid plate media	609:625	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	11	42	theme	strain	1515:1520	arg1	A3T					1522:1524	strain A3T	1515:1524	strain A3T	1515:1524	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	2	43	attach	isolated	136:143	arg2	A3T					127:129	A3T	127:129	A3T	127:129	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	2	43	attach	isolated	136:143	arg1	intestines					154:163	the intestines	150:163	the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark	150:245	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	2	43	attach	isolated	136:143	arg2	strain					119:124	A novel bacterial strain	101:124	A novel bacterial strain	101:124	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	5	44	theme	plate	615:619	arg1	media					621:625	solid plate media	609:625	solid plate media	609:625	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	10	45	theme	similarity	1174:1183	arg1	level					1142:1146	The level	1138:1146	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T)	1138:1311	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	10	45	theme	similarity	1174:1183	arg1	%					1322:1322	97.5 %	1317:1322	97.5 %	1317:1322	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	4	46	theme	carbon	530:535	arg1	source					537:542	sole carbon source	525:542	sole carbon source	525:542	Furthermore, strain A3T grew on agar, agarose, κ-carrageenan, alginate and laminarin as sole carbon source.
29388544	12	47	theme	genomic	1588:1594	arg1	analyses					1596:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses	1555:1603	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	5	48	theme	agar	570:573	arg1	result					632:637	a result	630:637	a result of enzymatic activities	630:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	48	theme	agar	570:573	arg1	liquefaction					554:565	Complete liquefaction	545:565	Complete liquefaction of agar and κ-carrageenan	545:591	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	8	49	theme	36.9 mol	954:961	arg1	content					942:948	The DNA G+C content	930:948	The DNA G+C content	930:948	The DNA G+C content was 36.9 mol%.
29388544	8	49	theme	36.9 mol	954:961	arg1	%					962:962	36.9 mol%	954:962	36.9 mol%	954:962	The DNA G+C content was 36.9 mol%.
29388544	12	50	theme	novel	1657:1661	arg1	species					1663:1669	a novel species	1655:1669	a novel species	1655:1669	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	10	51	theme	sequence	1165:1172	arg1	similarity					1174:1183	16S rRNA gene sequence similarity	1151:1183	16S rRNA gene sequence similarity between strain A3T and its closest relatives	1151:1228	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	12	52	theme	Colwellia	1684:1692	arg1	species					1663:1669	a novel species	1655:1669	a novel species	1655:1669	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	4	53	theme	strain	450:455	arg1	A3T					457:459	strain A3T	450:459	strain A3T	450:459	Furthermore, strain A3T grew on agar, agarose, κ-carrageenan, alginate and laminarin as sole carbon source.
29388544	8	54	theme	DNA	934:936	arg1	content					942:948	The DNA G+C content	930:948	The DNA G+C content	930:948	The DNA G+C content was 36.9 mol%.
29388544	8	54	theme	DNA	934:936	arg1	%					962:962	36.9 mol%	954:962	36.9 mol%	954:962	The DNA G+C content was 36.9 mol%.
29388544	2	55	theme	urchin	176:181	arg1	intestines					154:163	the intestines	150:163	the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark	150:245	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	10	56	theme	genus	1237:1241	arg1	Colwellia					1243:1251	the genus Colwellia	1233:1251	the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T)	1233:1311	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	10	57	theme	gene	1160:1163	arg1	similarity					1174:1183	16S rRNA gene sequence similarity	1151:1183	16S rRNA gene sequence similarity between strain A3T and its closest relatives	1151:1228	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	7	58	theme	respiratory	752:762	arg1	Q-8					807:809	ubiquinones Q-8	795:809	ubiquinones Q-8 (92 %)	795:816	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	7	58	theme	respiratory	752:762	arg1	quinones					764:771	The respiratory quinones	748:771	The respiratory quinones	748:771	The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol.
29388544	11	59	theme	DNA-DNA	1428:1434	arg1	hybridization					1436:1448	DNA-DNA hybridization	1428:1448	DNA-DNA hybridization prediction	1428:1459	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	5	60	theme	κ-carrageenan	579:591	arg1	result					632:637	a result	630:637	a result of enzymatic activities	630:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	5	60	theme	κ-carrageenan	579:591	arg1	liquefaction					554:565	Complete liquefaction	545:565	Complete liquefaction of agar and κ-carrageenan	545:591	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	2	61	theme	sea	172:174	arg1	urchin					176:181	the sea urchin	168:181	the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark	168:245	A novel bacterial strain, A3T, was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark.
29388544	10	62	from	level	1142:1146	arg1	Colwellia					1243:1251	the genus Colwellia	1233:1251	the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T)	1233:1311	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	9	63	theme	Phylogenetical	965:978	arg1	analyses					980:987	Phylogenetical analyses	965:987	Phylogenetical analyses based on the 16S rRNA gene	965:1014	Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria.
29388544	3	64	theme	w/v	412:414	arg1	NaCl					417:420	1-6 % (w/v) NaCl	405:420	1-6 % (w/v) NaCl (optimum 3 %)	405:434	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	3	64	theme	w/v	412:414	arg1	%					433:433	optimum 3 %	423:433	optimum 3 %	423:433	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	12	65	theme	genus	1678:1682	arg1	Colwellia					1684:1692	the genus Colwellia	1674:1692	the genus Colwellia	1674:1692	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3T represents a novel species of the genus Colwellia, for which the name Colwellia echini sp.
29388544	11	66	dep	%	1493:1493	arg1	23 					1490:1492	23 	1490:1492	23 	1490:1492	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	10	67	theme	rRNA	1155:1158	arg1	similarity					1174:1183	16S rRNA gene sequence similarity	1151:1183	16S rRNA gene sequence similarity between strain A3T and its closest relatives	1151:1228	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	0	68	dep	sp	17:18	arg1	echini					10:15	Colwellia echini	0:15	Colwellia echini	0:15	Colwellia echini sp.
29388544	8	69	theme	G+C	938:940	arg1	content					942:948	The DNA G+C content	930:948	The DNA G+C content	930:948	The DNA G+C content was 36.9 mol%.
29388544	8	69	theme	G+C	938:940	arg1	%					962:962	36.9 mol%	954:962	36.9 mol%	954:962	The DNA G+C content was 36.9 mol%.
29388544	11	70	dep	relatedness	1495:1505	arg1	%					1493:1493	%	1493:1493	%	1493:1493	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
29388544	3	71	from	pH	395:396	arg1	optimum					384:390	optimum	384:390	optimum at pH 7	384:398	The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %).
29388544	10	72	theme	closest	1212:1218	arg1	relatives					1220:1228	its closest relatives	1208:1228	its closest relatives	1208:1228	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	10	73	dep	Colwellia	1243:1251	arg1	ATCC					1273:1276	ATCC	1273:1276	ATCC	1273:1276	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	10	73	dep	Colwellia	1243:1251	arg1	002T					1307:1310	002T	1307:1310	002T	1307:1310	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	5	74	theme	enzymatic	642:650	arg1	activities					652:661	enzymatic activities	642:661	enzymatic activities	642:661	Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities.
29388544	9	75	theme	Gammaproteobacteria	1117:1135	arg1	Alteromonadaceae					1093:1108	the Alteromonadaceae	1089:1108	the Alteromonadaceae of the Gammaproteobacteria	1089:1135	Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria.
29388544	1	76	theme	agar-	30:34	arg1	bacterium					65:73	an agar- and carrageenan-solubilizing bacterium	27:73	an agar- and carrageenan-solubilizing bacterium	27:73	nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
29388544	1	76	theme	agar-	30:34	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
29388544	10	77	theme	strain	1193:1198	arg1	A3T					1200:1202	strain A3T	1193:1202	strain A3T	1193:1202	The level of 16S rRNA gene sequence similarity between strain A3T and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364T and C. asteriadis KMD 002T) was 97.5 %.
29388544	11	78	theme	relatedness	1495:1505	arg1	values					1470:1475	values	1470:1475	values of less than 23 % relatedness between strain A3T and other Colwellia species	1470:1552	The average nucleotide identity between strain A3T and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3T and other Colwellia species.
30530273	3	0	from	adsorption	372:381	arg1	hydrogel					434:441	the ALG based, 3D composite hydrogel	406:441	the ALG based, 3D composite hydrogel	406:441	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	1	1	theme	health	154:159	arg1	risk					161:164	severe health risk	147:164	severe health risk	147:164	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	4	2	theme	ALG/PEI	546:552	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	0	3	theme	Cu2+	92:95	arg1	utilization					77:87	utilization	77:87	utilization	77:87	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	0	3	theme	Cu2+	92:95	arg1	removal					65:71	cascaded removal	56:71	cascaded removal	56:71	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	4	4	theme	adsorption-catalysis	657:676	arg1	ability					678:684	a promising adsorption-catalysis ability	645:684	a promising adsorption-catalysis ability	645:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	4	5	theme	nanoparticle-loaded	526:544	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	3	6	dep	based	414:418	arg1	ALG					410:412	ALG	410:412	ALG	410:412	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	3	6	dep	based	414:418	arg1	3D					421:422	3D	421:422	3D	421:422	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	5	7	dep	treatment	779:787	arg1	the					766:768	the	766:768	the	766:768	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	2	8	theme	composite	268:276	arg1	hydrogel					278:285	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	0	9	theme	Pb2+	101:104	arg1	utilization					77:87	utilization	77:87	utilization	77:87	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	0	9	theme	Pb2+	101:104	arg1	removal					65:71	cascaded removal	56:71	cascaded removal	56:71	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	5	10	from	recycling	793:801	arg1	wastewater					826:835	wastewater	826:835	wastewater	826:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	4	11	theme	promising	647:655	arg1	adsorption-catalysis					657:676	a promising adsorption-catalysis	645:676	a promising adsorption-catalysis ability	645:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	4	12	theme	composite	554:562	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	5	13	theme	practical	743:751	arg1	paradigm					753:760	an effective and practical paradigm	726:760	an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater	726:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	5	14	theme	metal	812:816	arg1	ions					818:821	heavy metal ions	806:821	heavy metal ions in wastewater	806:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	4	15	theme	metal	630:634	arg1	ion					636:638	the heavy metal ion	620:638	the heavy metal ion with a promising adsorption-catalysis ability	620:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	5	16	from	treatment	779:787	arg1	wastewater					826:835	wastewater	826:835	wastewater	826:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	2	17	theme	/polyethyleneimine	243:260	arg1	hydrogel					278:285	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	1	18	theme	human	169:173	arg1	beings					175:180	human beings	169:180	human beings	169:180	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	0	19	theme	alginate/polyethyleneimine	6:31	arg1	hydrogel					33:40	Novel alginate/polyethyleneimine hydrogel	0:40	Novel alginate/polyethyleneimine hydrogel	0:40	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	4	20	dep	in	504:505	arg1	situ					507:510	situ	507:510	situ	507:510	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	3	21	theme	metal	392:396	arg1	ions					398:401	heavy metal ions	386:401	heavy metal ions	386:401	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	0	22	theme	Novel	0:4	arg1	hydrogel					33:40	Novel alginate/polyethyleneimine hydrogel	0:40	Novel alginate/polyethyleneimine hydrogel	0:40	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	0	23	dep	Cu2+	92:95	arg1	ions					106:109	ions	106:109	ions	106:109	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	2	24	theme	ions	346:349	arg1	removal					323:329	the removal	319:329	the removal of heavy metal ions from wastewater	319:365	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	4	25	theme	in	504:505	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	5	26	theme	heavy	806:810	arg1	ions					818:821	heavy metal ions	806:821	heavy metal ions in wastewater	806:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	4	27	theme	metal	520:524	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	1	28	theme	Heavy	112:116	arg1	pollution					128:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	4	29	theme	utilization	599:609	arg1	route					611:615	a sustainable utilization route	585:615	us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability	582:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	0	30	theme	cascaded	56:63	arg1	removal					65:71	cascaded removal	56:71	cascaded removal	56:71	Novel alginate/polyethyleneimine hydrogel adsorbent for cascaded removal and utilization of Cu2+ and Pb2+ ions.
30530273	4	31	theme	reduced	512:518	arg1	hydrogel					564:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel	500:571	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	4	32	with	ion	636:638	arg1	ability					678:684	a promising adsorption-catalysis ability	645:684	a promising adsorption-catalysis ability	645:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	2	33	theme	sodium	222:227	arg1	PEI					263:265	PEI	263:265	PEI	263:265	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	2	33	theme	sodium	222:227	arg1	/polyethyleneimine					243:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine	191:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	3	34	theme	ions	398:401	arg1	adsorption					372:381	The adsorption	368:381	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel	368:441	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	5	35	theme	ions	818:821	arg1	recycling					793:801	recycling	793:801	recycling	793:801	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	5	35	theme	ions	818:821	arg1	treatment					779:787	treatment	779:787	treatment	779:787	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	3	36	theme	heavy	386:390	arg1	ions					398:401	heavy metal ions	386:401	heavy metal ions	386:401	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	2	37	theme	efficient	212:220	arg1	PEI					263:265	PEI	263:265	PEI	263:265	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	2	37	theme	efficient	212:220	arg1	/polyethyleneimine					243:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine	191:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	5	38	from	ions	818:821	arg1	wastewater					826:835	wastewater	826:835	wastewater	826:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	3	39	theme	based	414:418	arg1	hydrogel					434:441	the ALG based, 3D composite hydrogel	406:441	the ALG based, 3D composite hydrogel	406:441	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	1	40	theme	metal	118:122	arg1	pollution					128:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	5	41	theme	cascaded	770:777	arg1	treatment					779:787	treatment	779:787	treatment	779:787	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	2	42	theme	natural	193:199	arg1	PEI					263:265	PEI	263:265	PEI	263:265	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	2	42	theme	natural	193:199	arg1	/polyethyleneimine					243:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine	191:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	1	43	theme	ion	124:126	arg1	pollution					128:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution	112:136	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	4	44	theme	ion	636:638	arg1	route					611:615	a sustainable utilization route	585:615	us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability	582:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	2	45	from	wastewater	356:365	arg1	removal					323:329	the removal	319:329	the removal of heavy metal ions from wastewater	319:365	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	3	46	theme	composite	424:432	arg1	hydrogel					434:441	the ALG based, 3D composite hydrogel	406:441	the ALG based, 3D composite hydrogel	406:441	The adsorption of heavy metal ions on the ALG based, 3D composite hydrogel were thoroughly investigated in this study.
30530273	5	47	from	wastewater	826:835	arg1	recycling					793:801	recycling	793:801	recycling	793:801	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	5	47	from	wastewater	826:835	arg1	treatment					779:787	treatment	779:787	treatment	779:787	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	4	48	dep	us	582:583	arg1	route					611:615	a sustainable utilization route	585:615	us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability	582:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	2	49	theme	ALG	239:241	arg1	PEI					263:265	PEI	263:265	PEI	263:265	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	2	49	theme	ALG	239:241	arg1	/polyethyleneimine					243:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine	191:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	1	50	theme	severe	147:152	arg1	risk					161:164	severe health risk	147:164	severe health risk	147:164	Heavy metal ion pollution leads to severe health risk to human beings.
30530273	2	51	theme	metal	340:344	arg1	ions					346:349	heavy metal ions	334:349	heavy metal ions	334:349	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	4	52	theme	heavy	624:628	arg1	ion					636:638	the heavy metal ion	620:638	the heavy metal ion with a promising adsorption-catalysis ability	620:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	4	53	theme	sustainable	587:597	arg1	route					611:615	a sustainable utilization route	585:615	us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability	582:684	Furthermore, the in situ reduced metal nanoparticle-loaded ALG/PEI composite hydrogel provided us a sustainable utilization route of the heavy metal ion with a promising adsorption-catalysis ability.
30530273	5	54	theme	effective	729:737	arg1	paradigm					753:760	an effective and practical paradigm	726:760	an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater	726:835	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	2	55	theme	alginate	229:236	arg1	PEI					263:265	PEI	263:265	PEI	263:265	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	2	55	theme	alginate	229:236	arg1	/polyethyleneimine					243:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine	191:260	a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel	191:285	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
30530273	5	56	attach	present	718:724	arg1	general					690:696	general	690:696	general	690:696	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	5	56	attach	present	718:724	arg2	research					704:711	this research	699:711	this research	699:711	In general, this research will present an effective and practical paradigm for the cascaded treatment and recycling of heavy metal ions in wastewater.
30530273	2	57	theme	heavy	334:338	arg1	ions					346:349	heavy metal ions	334:349	heavy metal ions	334:349	Herein, a natural and highly efficient sodium alginate (ALG)/polyethyleneimine (PEI) composite hydrogel was designed and fabricated for the removal of heavy metal ions from wastewater.
31570190	7	0	theme	0.176	1458:1462	arg1	kkd					1464:1466	0.176 kkd	1458:1466	0.176 kkd	1458:1466	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	10	1	theme	ACD	1827:1829	arg1	injection					1831:1839	ACD injection	1827:1839	ACD injection	1827:1839	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	6	2	theme	high	1250:1253	arg1	productivity					1255:1266	high productivity	1250:1266	high productivity	1250:1266	The parameters were optimized to determine a method yielding high productivity or reduced environmental impact.
31570190	8	3	theme	56,414	1642:1647	arg1	Factor					1618:1623	an E Factor	1613:1623	an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration)	1613:1708	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	8	4	theme	environmental	1587:1599	arg1	impact					1601:1606	the worst environmental impact	1577:1606	the worst environmental impact	1577:1606	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	5	5	theme	injection	1178:1186	arg1	modes					1169:1173	stacked modes	1161:1173	stacked modes of injection	1161:1186	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	5	5	theme	injection	1178:1186	arg1	sizes					1138:1142	four column sizes	1126:1142	four column sizes for sequence	1126:1155	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	7	6	theme	sequential	1387:1396	arg1	mode					1398:1401	the sequential mode	1383:1401	the sequential mode	1383:1401	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	1	7	theme	chromatography	174:187	arg1	advent					144:149	The advent	140:149	The advent of supercritical fluid chromatography (SFC) in the 90s	140:204	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	4	8	theme	/CO2	889:892	arg1	v/v					900:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	8	theme	/CO2	889:892	arg1	phase					918:922	the mobile phase	907:922	the mobile phase	907:922	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	9	theme	Chiralpak®	677:686	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	5	10	dep	productivity	949:960	arg1	kilograms					963:971	kilograms	963:971	kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd	963:1046	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	10	11	theme	48 min	1878:1883	arg1	915 mL					1842:1847	915 mL	1842:1847	915 mL of ethanol and approximately 48 min	1842:1883	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	10	12	theme	mixed	1913:1917	arg1	injection					1926:1934	mixed stream injection	1913:1934	mixed stream injection	1913:1934	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	6	13	theme	environmental	1279:1291	arg1	impact					1293:1298	reduced environmental impact	1271:1298	reduced environmental impact	1271:1298	The parameters were optimized to determine a method yielding high productivity or reduced environmental impact.
31570190	3	14	theme	column	603:608	arg1	size					610:613	column size	603:613	column size	603:613	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	7	15	theme	injection	1324:1332	arg1	mode					1316:1319	the stacked mode	1304:1319	the stacked mode	1304:1319	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	4	16	theme	stationary	756:765	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	17	dep	phase	767:771	arg1	columns					773:779	columns	773:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	18	theme	amylose	693:699	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	5	19	theme	E	1096:1096	arg1	factor					1088:1093	environmental factor	1074:1093	environmental factor (E Factor)	1074:1104	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	5	19	theme	E	1096:1096	arg1	Factor					1098:1103	E Factor	1096:1103	E Factor	1096:1103	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	10	20	theme	63 min	1960:1965	arg1	1200 mL					1937:1943	1200 mL	1937:1943	1200 mL of ethanol and 63 min	1937:1965	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	0	21	theme	chiral	85:90	arg1	separations					92:102	preparative chiral separations	73:102	preparative chiral separations	73:102	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	1	22	theme	supercritical	154:166	arg1	SFC					190:192	SFC	190:192	SFC	190:192	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	1	22	theme	supercritical	154:166	arg1	chromatography					174:187	supercritical fluid chromatography	154:187	supercritical fluid chromatography (SFC)	154:193	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	0	23	from	Productivity	0:11	arg1	chromatography					54:67	supercritical fluid chromatography	34:67	supercritical fluid chromatography	34:67	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	10	24	theme	ethanol	1948:1954	arg1	1200 mL					1937:1943	1200 mL	1937:1943	1200 mL of ethanol and 63 min	1937:1965	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	5	25	theme	racemate	976:983	arg1	kilograms					963:971	kilograms	963:971	kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd	963:1046	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	2	26	theme	enantiomers	386:396	arg1	grams					372:376	one hundred grams	360:376	one hundred grams of pure enantiomers	360:396	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	7	27	theme	stacked	1308:1314	arg1	mode					1316:1319	the stacked mode	1304:1319	the stacked mode	1304:1319	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	3	28	dep	raises	511:516	arg1	concerns					518:525	concerns	518:525	raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition	511:659	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	2	29	theme	higher	434:439	arg1	quantities					441:450	higher quantities	434:450	higher quantities	434:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	4	30	theme	various	784:790	arg1	dimensions					792:801	various dimensions	784:801	various dimensions	784:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	0	31	theme	solvent	17:23	arg1	waste					25:29	solvent waste	17:29	solvent waste	17:29	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	3	32	theme	processing	537:546	arg1	volumes					580:586	injection volumes	570:586	injection volumes	570:586	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	3	32	theme	processing	537:546	arg1	parameters					548:557	processing parameters	537:557	processing parameters	537:557	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	3	32	theme	processing	537:546	arg1	frequency					592:600	frequency	592:600	frequency	592:600	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	9	33	theme	stream	1746:1751	arg1	mode					1763:1766	mixed stream injection mode	1740:1766	mixed stream injection mode	1740:1766	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	4	34	theme	EtOH	863:866	arg1	v/v					900:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	34	theme	EtOH	863:866	arg1	phase					918:922	the mobile phase	907:922	the mobile phase	907:922	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	8	35	theme	production	1525:1534	arg1	rate					1536:1539	the best production rate	1516:1539	the best production rate of 467 mg purified per hour	1516:1567	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	0	36	theme	fluid	48:52	arg1	chromatography					54:67	supercritical fluid chromatography	34:67	supercritical fluid chromatography	34:67	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	2	37	theme	discovery	320:328	arg1	processes					330:338	drug discovery processes	315:338	drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities	315:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	7	38	theme	kkd	1464:1466	arg1	productivity					1442:1453	the best productivity	1433:1453	the best productivity of 0.176 kkd	1433:1466	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	4	39	theme	AD-H	688:691	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	40	theme	propranolol	845:855	arg1	purification					829:840	the purification	825:840	the purification of propranolol	825:855	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	1	41	theme	preparative	218:228	arg1	chromatography					237:250	preparative liquid chromatography	218:250	preparative liquid chromatography	218:250	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	5	42	theme	stationary	1018:1027	arg1	phase					1029:1033	chiral stationary phase	1011:1033	chiral stationary phase	1011:1033	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	3	43	theme	mixture	503:509	arg1	45 mg					486:490	45 mg	486:490	45 mg of racemic mixture	486:509	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	5	44	theme	stacked	1161:1167	arg1	modes					1169:1173	stacked modes	1161:1173	stacked modes of injection	1161:1186	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	7	45	theme	best	1437:1440	arg1	productivity					1442:1453	the best productivity	1433:1453	the best productivity of 0.176 kkd	1433:1466	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	4	46	theme	15/85	894:898	arg1	v/v					900:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	46	theme	15/85	894:898	arg1	phase					918:922	the mobile phase	907:922	the mobile phase	907:922	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	9	47	theme	propranolol	1809:1819	arg1	495 mg					1799:1804	separate 495 mg	1790:1804	separate 495 mg of propranolol	1790:1819	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	8	48	theme	worst	1581:1585	arg1	impact					1601:1606	the worst environmental impact	1577:1606	the worst environmental impact	1577:1606	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	10	49	theme	ethanol	1852:1858	arg1	915 mL					1842:1847	915 mL	1842:1847	915 mL of ethanol and approximately 48 min	1842:1883	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	2	50	theme	improved	263:270	arg1	way					272:274	an improved way	260:274	an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities	260:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	2	50	theme	improved	263:270	arg1	SFC					253:255	SFC	253:255	SFC	253:255	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	1	51	theme	fluid	168:172	arg1	SFC					190:192	SFC	190:192	SFC	190:192	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	1	51	theme	fluid	168:172	arg1	chromatography					174:187	supercritical fluid chromatography	154:187	supercritical fluid chromatography (SFC)	154:193	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	7	52	theme	shortest	1408:1415	arg1	column					1417:1422	the shortest column	1404:1422	the shortest column	1404:1422	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	4	53	theme	%	873:873	arg1	EtOH					863:866	EtOH	863:866	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	53	theme	%	873:873	arg1	triethylamine					875:887	+0.3% triethylamine	869:887	+0.3% triethylamine	869:887	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	0	54	theme	convenient	119:128	arg1	strategy					130:137	a convenient strategy	117:137	a convenient strategy	117:137	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	2	55	theme	chiral	291:296	arg1	compounds					298:306	chiral compounds	291:306	chiral compounds	291:306	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	8	56	theme	solvent	1661:1667	arg1	volume					1669:1674	the solvent volume	1657:1674	the solvent volume used during column equilibration	1657:1707	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	5	57	theme	Production	925:934	arg1	mg/h					942:945	mg/h	942:945	mg/h	942:945	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	5	57	theme	Production	925:934	arg1	rate					936:939	Production rate	925:939	Production rate (mg/h)	925:946	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	3	58	theme	racemic	495:501	arg1	mixture					503:509	racemic mixture	495:509	racemic mixture	495:509	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	7	59	theme	rapid	1352:1356	arg1	processing					1358:1367	rapid processing	1352:1367	rapid processing	1352:1367	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	0	60	dep	Productivity	0:11	arg1	guide					107:111	a guide	105:111	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.	0:138	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	8	61	theme	column	1688:1693	arg1	equilibration					1695:1707	column equilibration	1688:1707	column equilibration	1688:1707	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	4	62	theme	mobile	911:916	arg1	v/v					900:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	EtOH (+0.3% triethylamine)/CO2 15/85 v/v	863:902	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	62	theme	mobile	911:916	arg1	phase					918:922	the mobile phase	907:922	the mobile phase	907:922	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	4	63	theme	3,5-dimethylphenylcarbamate	706:732	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	6	64	theme	reduced	1271:1277	arg1	impact					1293:1298	reduced environmental impact	1271:1298	reduced environmental impact	1271:1298	The parameters were optimized to determine a method yielding high productivity or reduced environmental impact.
31570190	3	65	theme	injection	570:578	arg1	volumes					580:586	injection volumes	570:586	injection volumes	570:586	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	4	66	theme	polysaccharide-based	735:754	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	0	67	theme	preparative	73:83	arg1	separations					92:102	preparative chiral separations	73:102	preparative chiral separations	73:102	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	5	68	theme	solvent	1050:1056	arg1	L/g					1065:1067	L/g	1065:1067	L/g	1065:1067	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	5	68	theme	solvent	1050:1056	arg1	usage					1058:1062	solvent usage	1050:1062	solvent usage (L/g)	1050:1068	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	3	69	theme	chromatographic	616:630	arg1	method					632:637	chromatographic method	616:637	chromatographic method	616:637	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	0	70	from	waste	25:29	arg1	chromatography					54:67	supercritical fluid chromatography	34:67	supercritical fluid chromatography	34:67	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	5	71	dep	kilograms	963:971	arg1	kkd					1044:1046	kkd	1044:1046	kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd	963:1046	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	10	72	theme	stream	1919:1924	arg1	injection					1926:1934	mixed stream injection	1913:1934	mixed stream injection	1913:1934	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	8	73	theme	semi-preparative	1471:1486	arg1	column					1488:1493	A semi-preparative column	1469:1493	A semi-preparative column (30 mm i.d.)	1469:1506	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	8	73	theme	semi-preparative	1471:1486	arg1	i.d.					1502:1505	30 mm i.d.	1496:1505	30 mm i.d.	1496:1505	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	4	74	theme	tris	701:704	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	9	75	theme	Column	1714:1719	arg1	ACD					1731:1733	ACD	1731:1733	ACD	1731:1733	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	9	75	theme	Column	1714:1719	arg1	Dilution					1721:1728	Column Dilution	1714:1728	Column Dilution (ACD)	1714:1734	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	8	76	contain	had	1573:1575	arg2	impact					1601:1606	the worst environmental impact	1577:1606	the worst environmental impact	1577:1606	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	8	76	contain	had	1573:1575	arg1	column					1488:1493	A semi-preparative column	1469:1493	A semi-preparative column (30 mm i.d.)	1469:1506	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	8	76	contain	had	1573:1575	arg1	i.d.					1502:1505	30 mm i.d.	1496:1505	30 mm i.d.	1496:1505	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	4	77	theme	dimensions	792:801	arg1	phase					767:771	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns	677:779	Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions	677:801	In this study, Chiralpak® AD-H amylose tris(3,5-dimethylphenylcarbamate) polysaccharide-based stationary phase columns of various dimensions were investigated for the purification of propranolol using EtOH (+0.3% triethylamine)/CO2 15/85 v/v as the mobile phase.
31570190	5	78	theme	environmental	1074:1086	arg1	factor					1088:1093	environmental factor	1074:1093	environmental factor (E Factor)	1074:1104	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	5	78	theme	environmental	1074:1086	arg1	Factor					1098:1103	E Factor	1096:1103	E Factor	1096:1103	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	1	79	from	advent	144:149	arg1	90s					202:204	the 90s	198:204	the 90s	198:204	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31570190	10	80	dep	required	1890:1897	arg1	whereas					1900:1906	whereas	1900:1906	whereas	1900:1906	With ACD injection, 915 mL of ethanol and approximately 48 min were required, whereas with mixed stream injection, 1200 mL of ethanol and 63 min were required.
31570190	2	81	theme	pure	381:384	arg1	enantiomers					386:396	pure enantiomers	381:396	pure enantiomers	381:396	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	2	82	theme	grams	372:376	arg1	upwards					349:355	upwards	349:355	upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities	349:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	9	83	theme	mixed	1740:1744	arg1	mode					1763:1766	mixed stream injection mode	1740:1766	mixed stream injection mode	1740:1766	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	8	84	theme	best	1520:1523	arg1	rate					1536:1539	the best production rate	1516:1539	the best production rate of 467 mg purified per hour	1516:1567	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	2	85	theme	clinical	408:415	arg1	trials					417:422	clinical trials	408:422	clinical trials requiring higher quantities	408:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	0	86	theme	supercritical	34:46	arg1	chromatography					54:67	supercritical fluid chromatography	34:67	supercritical fluid chromatography	34:67	Productivity and solvent waste in supercritical fluid chromatography for preparative chiral separations: a guide for a convenient strategy.
31570190	5	87	theme	phase	1029:1033	arg1	kilogram					999:1006	kilogram	999:1006	kilogram of chiral stationary phase per day	999:1041	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	9	88	theme	injection	1753:1761	arg1	mode					1763:1766	mixed stream injection mode	1740:1766	mixed stream injection mode	1740:1766	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	3	89	theme	feed	644:647	arg1	composition					649:659	feed composition	644:659	feed composition	644:659	The need to purify approximately 45 mg of racemic mixture raises concerns regarding processing parameters, including injection volumes and frequency, column size, chromatographic method, and feed composition.
31570190	8	90	theme	467 mg	1544:1549	arg1	rate					1536:1539	the best production rate	1516:1539	the best production rate of 467 mg purified per hour	1516:1567	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	7	91	attach	presents	1424:1431	arg1	mode					1316:1319	the stacked mode	1304:1319	the stacked mode	1304:1319	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	7	91	attach	presents	1424:1431	arg2	column					1417:1422	the shortest column	1404:1422	the shortest column	1404:1422	In the stacked mode of injection, which allows for rapid processing compared with the sequential mode, the shortest column presents the best productivity of 0.176 kkd.
31570190	5	92	theme	column	1131:1136	arg1	sizes					1138:1142	four column sizes	1126:1142	four column sizes for sequence	1126:1155	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	2	93	theme	drug	315:318	arg1	processes					330:338	drug discovery processes	315:338	drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities	315:450	SFC is an improved way for separating chiral compounds during drug discovery processes yielding upwards of one hundred grams of pure enantiomers or during clinical trials requiring higher quantities.
31570190	8	94	theme	E	1616:1616	arg1	Factor					1618:1623	an E Factor	1613:1623	an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration)	1613:1708	A semi-preparative column (30 mm i.d.) yielded the best production rate of 467 mg purified per hour but had the worst environmental impact with an E Factor of approximately 56,414 (due to the solvent volume used during column equilibration).
31570190	5	95	theme	chiral	1011:1016	arg1	phase					1029:1033	chiral stationary phase	1011:1033	chiral stationary phase	1011:1033	Production rate (mg/h), productivity (kilograms of racemate separated per kilogram of chiral stationary phase per day; kkd), solvent usage (L/g) and environmental factor (E Factor) were calculated for four column sizes for sequence and stacked modes of injection.
31570190	9	96	theme	separate	1790:1797	arg1	495 mg					1799:1804	separate 495 mg	1790:1804	separate 495 mg of propranolol	1790:1819	At Column Dilution (ACD) and mixed stream injection mode were also compared to separate 495 mg of propranolol.
31570190	1	97	theme	liquid	230:235	arg1	chromatography					237:250	preparative liquid chromatography	218:250	preparative liquid chromatography	218:250	The advent of supercritical fluid chromatography (SFC) in the 90s has changed preparative liquid chromatography.
31798864	2	0	theme	functional	318:327	arg1	capacity					329:336	functional capacity	318:336	functional capacity	318:336	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	6	1	theme	amplicon	859:866	arg1	sequencing					868:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	4	2	theme	Buchinger	574:582	arg1	guidelines					592:601	the peer-reviewed Buchinger fasting guidelines	556:601	the peer-reviewed Buchinger fasting guidelines	556:601	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	3	3	theme	men	518:520	arg1	microbiota					488:497	the faecal microbiota	477:497	the faecal microbiota of fifteen healthy men	477:520	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	6	4	theme	rRNA	849:852	arg1	sequencing					868:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	11	5	theme	inflammatory	1549:1560	arg1	status					1562:1567	inflammatory status	1549:1567	inflammatory status	1549:1567	We also monitored intestinal permeability and inflammatory status.
31798864	8	6	from	Changes	1192:1198	arg1	abundance					1208:1216	taxa abundance	1203:1216	taxa abundance	1203:1216	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	8	7	theme	serum	1239:1243	arg1	glucose					1245:1251	serum glucose	1239:1251	serum glucose	1239:1251	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	0	8	attach	linked	48:53	arg2	Changes					0:6	Changes	0:6	Changes in human gut microbiota composition	0:42	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	0	8	attach	linked	48:53	arg1	switch					79:84	the energy metabolic switch	58:84	the energy metabolic switch	58:84	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	13	9	theme	gut	1744:1746	arg1	microbiota					1748:1757	the gut microbiota	1740:1757	the gut microbiota	1740:1757	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	7	10	theme	concomitant	1044:1054	arg1	increase					1056:1063	a concomitant increase	1042:1063	a concomitant increase	1042:1063	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	5	11	theme	Emotional	789:797	arg1	well-being					812:821	Emotional and physical well-being	789:821	Emotional and physical well-being	789:821	Emotional and physical well-being were enhanced.
31798864	12	12	theme	γ	1594:1594	arg1	levels					1605:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	8	13	theme	branched-chain	1264:1277	arg1	BCAA					1292:1295	BCAA	1292:1295	BCAA	1292:1295	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	8	13	theme	branched-chain	1264:1277	arg1	acids					1285:1289	faecal branched-chain amino acids	1257:1289	faecal branched-chain amino acids (BCAA)	1257:1296	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	0	14	theme	fasting	111:117	arg1	d					96:96	10 d	93:96	10 d of Buchinger fasting	93:117	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	5	15	theme	physical	803:810	arg1	well-being					812:821	Emotional and physical well-being	789:821	Emotional and physical well-being	789:821	Emotional and physical well-being were enhanced.
31798864	12	16	theme	postprandial	1683:1694	arg1	response					1703:1710	the postprandial immune response	1679:1710	the postprandial immune response	1679:1710	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	4	17	theme	enema	675:679	arg1	biochemistry					698:709	an enema every 2 d. Serum biochemistry	672:709	an enema every 2 d. Serum biochemistry	672:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	7	18	link	host-derived	1160:1171	arg1	substrates					1180:1189	host-derived energy substrates	1160:1189	host-derived energy substrates	1160:1189	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	12	19	theme	response	1703:1710	arg1	reactivation					1663:1674	a reactivation	1661:1674	a reactivation of the postprandial immune response	1661:1710	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	2	20	theme	human	225:229	arg1	microbiota					235:244	the human gut microbiota	221:244	the human gut microbiota	221:244	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	6	21	theme	dietary	963:969	arg1	Ruminococcaceae					1015:1029	Ruminococcaceae	1015:1029	Ruminococcaceae	1015:1029	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	6	21	theme	dietary	963:969	arg1	polysaccharides					971:985	dietary polysaccharides	963:985	dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae	963:1029	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	6	21	theme	dietary	963:969	arg1	Lachnospiraceae					995:1009	Lachnospiraceae	995:1009	Lachnospiraceae	995:1009	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	13	22	theme	health	1868:1873	arg1	effects					1875:1881	its beneficial health effects	1853:1881	its beneficial health effects	1853:1881	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	0	23	theme	energy	62:67	arg1	switch					79:84	the energy metabolic switch	58:84	the energy metabolic switch	58:84	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	0	24	from	Changes	0:6	arg1	composition					32:42	human gut microbiota composition	11:42	human gut microbiota composition	11:42	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	6	25	theme	bacteria	937:944	arg1	abundance					924:932	the abundance	920:932	the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae	920:1029	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	4	26	theme	metabolic	725:733	arg1	switch					735:740	the metabolic switch	721:740	the metabolic switch from carbohydrates to fatty acids and ketones	721:786	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	2	27	theme	external	372:379	arg1	supply					390:395	the external nutrient supply	368:395	the external nutrient supply	368:395	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	7	28	theme	energy	1173:1178	arg1	substrates					1180:1189	host-derived energy substrates	1160:1189	host-derived energy substrates	1160:1189	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	13	29	from	changes	1729:1735	arg1	microbiota					1748:1757	the gut microbiota	1740:1757	the gut microbiota	1740:1757	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	10	30	theme	SCFA	1449:1452	arg1	levels					1454:1459	SCFA levels	1449:1459	SCFA levels	1449:1459	SCFA levels were unchanged at the end of the fasting.
31798864	4	31	theme	Serum	692:696	arg1	biochemistry					698:709	an enema every 2 d. Serum biochemistry	672:709	an enema every 2 d. Serum biochemistry	672:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	13	32	theme	10-d	1806:1809	arg1	fasting					1820:1826	a 10-d periodic fasting	1804:1826	a 10-d periodic fasting	1804:1826	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	0	33	theme	human	11:15	arg1	composition					32:42	human gut microbiota composition	11:42	human gut microbiota composition	11:42	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	8	34	theme	host	1381:1384	arg1	metabolism					1393:1402	host energy metabolism	1381:1402	host energy metabolism	1381:1402	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	0	35	theme	microbiota	21:30	arg1	composition					32:42	human gut microbiota composition	11:42	human gut microbiota composition	11:42	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	3	36	theme	periodic	457:464	arg1	fasting					466:472	a 10-d periodic fasting	450:472	a 10-d periodic fasting	450:472	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	7	37	theme	Bilophila	1123:1131	arg1	wadsworthia					1133:1143	Bilophila wadsworthia	1123:1143	Bilophila wadsworthia	1123:1143	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	6	38	from	decrease	908:915	arg1	abundance					924:932	the abundance	920:932	the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae	920:1029	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	4	39	theme	fasting	584:590	arg1	guidelines					592:601	the peer-reviewed Buchinger fasting guidelines	556:601	the peer-reviewed Buchinger fasting guidelines	556:601	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	2	40	theme	prolonged	345:353	arg1	fasting					355:361	prolonged fasting	345:361	prolonged fasting	345:361	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	13	41	theme	adaptations	1789:1799	arg1	part					1763:1766	part	1763:1766	part of the physiological adaptations	1763:1799	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	13	41	theme	adaptations	1789:1799	arg1	changes					1729:1735	changes	1729:1735	changes in the gut microbiota	1729:1757	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	6	42	theme	Faecal	838:843	arg1	sequencing					868:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	11	43	theme	intestinal	1521:1530	arg1	permeability					1532:1543	intestinal permeability	1521:1543	intestinal permeability	1521:1543	We also monitored intestinal permeability and inflammatory status.
31798864	3	44	theme	healthy	510:516	arg1	men					518:520	fifteen healthy men	502:520	fifteen healthy men	502:520	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	6	45	theme	gene	854:857	arg1	sequencing					868:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	4	46	theme	peer-reviewed	560:572	arg1	guidelines					592:601	the peer-reviewed Buchinger fasting guidelines	556:601	the peer-reviewed Buchinger fasting guidelines	556:601	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	6	47	theme	16S	845:847	arg1	sequencing					868:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing	838:877	Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae.
31798864	7	48	dep	Proteobacteria	1086:1099	arg1	wadsworthia					1133:1143	Bilophila wadsworthia	1123:1143	Bilophila wadsworthia	1123:1143	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	7	48	dep	Proteobacteria	1086:1099	arg1	coli					1114:1117	Escherichia coli	1102:1117	Escherichia coli	1102:1117	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	1	49	theme	metabolic	162:170	arg1	diseases					189:196	metabolic and inflammatory diseases	162:196	metabolic and inflammatory diseases	162:196	Fasting is increasingly popular to manage metabolic and inflammatory diseases.
31798864	12	50	theme	interferon	1583:1592	arg1	γ					1594:1594	interferon γ	1583:1594	interferon γ	1583:1594	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	0	51	theme	Buchinger	101:109	arg1	fasting					111:117	Buchinger fasting	101:117	Buchinger fasting	101:117	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	1	52	theme	inflammatory	176:187	arg1	diseases					189:196	metabolic and inflammatory diseases	162:196	metabolic and inflammatory diseases	162:196	Fasting is increasingly popular to manage metabolic and inflammatory diseases.
31798864	12	53	theme	IL-6	1570:1573	arg1	levels					1605:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	8	54	theme	faecal	1257:1262	arg1	BCAA					1292:1295	BCAA	1292:1295	BCAA	1292:1295	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	8	54	theme	faecal	1257:1262	arg1	acids					1285:1289	faecal branched-chain amino acids	1257:1289	faecal branched-chain amino acids (BCAA)	1257:1296	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	8	55	theme	taxa	1203:1206	arg1	abundance					1208:1216	taxa abundance	1203:1216	taxa abundance	1203:1216	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	12	56	theme	IL-10	1576:1580	arg1	levels					1605:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	8	57	theme	amino	1279:1283	arg1	BCAA					1292:1295	BCAA	1292:1295	BCAA	1292:1295	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	8	57	theme	amino	1279:1283	arg1	acids					1285:1289	faecal branched-chain amino acids	1257:1289	faecal branched-chain amino acids (BCAA)	1257:1296	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	2	58	theme	gut	231:233	arg1	microbiota					235:244	the human gut microbiota	221:244	the human gut microbiota	221:244	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	4	59	from	carbohydrates	747:759	arg1	switch					735:740	the metabolic switch	721:740	the metabolic switch from carbohydrates to fatty acids and ketones	721:786	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	12	60	theme	immune	1696:1701	arg1	response					1703:1710	the postprandial immune response	1679:1710	the postprandial immune response	1679:1710	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	10	61	from	end	1483:1485	arg1	unchanged					1466:1474	unchanged	1466:1474	unchanged	1466:1474	SCFA levels were unchanged at the end of the fasting.
31798864	4	62	theme	kJ	654:655	arg1	intake					633:638	a daily energy intake	618:638	a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry	618:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	13	63	theme	beneficial	1857:1866	arg1	effects					1875:1881	its beneficial health effects	1853:1881	its beneficial health effects	1853:1881	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	0	64	theme	metabolic	69:77	arg1	switch					79:84	the energy metabolic switch	58:84	the energy metabolic switch	58:84	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	8	65	theme	fasting-induced	1315:1329	arg1	changes					1331:1337	fasting-induced changes	1315:1337	fasting-induced changes in the gut microbiota	1315:1359	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	4	66	theme	biochemistry	698:709	arg1	intake					633:638	a daily energy intake	618:638	a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry	618:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	7	67	theme	host-derived	1160:1171	arg1	substrates					1180:1189	host-derived energy substrates	1160:1189	host-derived energy substrates	1160:1189	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	7	68	from	increase	1056:1063	arg1	Bacteroidetes					1068:1080	Bacteroidetes	1068:1080	Bacteroidetes	1068:1080	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	7	68	from	increase	1056:1063	arg1	Proteobacteria					1086:1099	Proteobacteria	1086:1099	Proteobacteria (Escherichia coli and Bilophila wadsworthia)	1086:1144	There was a concomitant increase in Bacteroidetes and Proteobacteria (Escherichia coli and Bilophila wadsworthia), known to use host-derived energy substrates.
31798864	8	69	theme	gut	1346:1348	arg1	microbiota					1350:1359	the gut microbiota	1342:1359	the gut microbiota	1342:1359	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	0	70	theme	gut	17:19	arg1	composition					32:42	human gut microbiota composition	11:42	human gut microbiota composition	11:42	Changes in human gut microbiota composition are linked to the energy metabolic switch during 10 d of Buchinger fasting.
31798864	12	71	theme	TNFα	1600:1603	arg1	levels					1605:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels	1570:1610	IL-6, IL-10, interferon γ and TNFα levels increased when food was reintroduced, suggesting a reactivation of the postprandial immune response.
31798864	4	72	theme	d.	689:690	arg1	biochemistry					698:709	an enema every 2 d. Serum biochemistry	672:709	an enema every 2 d. Serum biochemistry	672:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	13	73	theme	periodic	1811:1818	arg1	fasting					1820:1826	a 10-d periodic fasting	1804:1826	a 10-d periodic fasting	1804:1826	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
31798864	3	74	theme	faecal	481:486	arg1	microbiota					488:497	the faecal microbiota	477:497	the faecal microbiota of fifteen healthy men	477:520	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	4	75	theme	energy	626:631	arg1	intake					633:638	a daily energy intake	618:638	a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry	618:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	4	76	theme	fatty	764:768	arg1	acids					770:774	fatty acids	764:774	fatty acids	764:774	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	2	77	theme	nutrient	381:388	arg1	supply					390:395	the external nutrient supply	368:395	the external nutrient supply	368:395	Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed.
31798864	10	78	theme	fasting	1494:1500	arg1	end					1483:1485	the end	1479:1485	the end of the fasting	1479:1500	SCFA levels were unchanged at the end of the fasting.
31798864	8	79	theme	energy	1386:1391	arg1	metabolism					1393:1402	host energy metabolism	1381:1402	host energy metabolism	1381:1402	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	3	80	from	effects	439:445	arg1	microbiota					488:497	the faecal microbiota	477:497	the faecal microbiota of fifteen healthy men	477:520	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	3	81	theme	fasting	466:472	arg1	effects					439:445	the effects	435:445	the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men	435:520	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	3	82	theme	10-d	452:455	arg1	fasting					466:472	a 10-d periodic fasting	450:472	a 10-d periodic fasting	450:472	We analysed the effects of a 10-d periodic fasting on the faecal microbiota of fifteen healthy men.
31798864	8	83	from	changes	1331:1337	arg1	microbiota					1350:1359	the gut microbiota	1342:1359	the gut microbiota	1342:1359	Changes in taxa abundance were associated with serum glucose and faecal branched-chain amino acids (BCAA), suggesting that fasting-induced changes in the gut microbiota are associated with host energy metabolism.
31798864	4	84	theme	daily	620:624	arg1	intake					633:638	a daily energy intake	618:638	a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry	618:709	Participants fasted according to the peer-reviewed Buchinger fasting guidelines, which involve a daily energy intake of about 1046 kJ (250 kcal) and an enema every 2 d. Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones.
31798864	13	85	theme	physiological	1775:1787	arg1	adaptations					1789:1799	the physiological adaptations	1771:1799	the physiological adaptations	1771:1799	We suggest that changes in the gut microbiota are part of the physiological adaptations to a 10-d periodic fasting, potentially influencing its beneficial health effects.
29410268	7	0	theme	storage	1151:1157	arg1	modulus					1159:1165	The storage modulus	1147:1165	The storage modulus	1147:1165	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	4	1	theme	infrared	560:567	arg1	spectroscopy					569:580	Fourier-transform infrared spectroscopy	542:580	Fourier-transform infrared spectroscopy (FT-IR)	542:588	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	4	1	theme	infrared	560:567	arg1	FT-IR					583:587	FT-IR	583:587	FT-IR	583:587	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	9	2	theme	TPS-based	1475:1483	arg1	nanocomposites					1485:1498	TPS-based nanocomposites	1475:1498	TPS-based nanocomposites	1475:1498	In addition, TGA and DSC results showed that increasing CNF content improves the thermal stability of TPS-based nanocomposites.
29410268	3	3	with	Nanocomposites	398:411	arg1	%					436:436	0.5, 1.0 and 1.5wt%	418:436	0.5, 1.0 and 1.5wt% of CNFs content	418:452	Nanocomposites with 0.5, 1.0 and 1.5wt% of CNFs content were prepared.
29410268	3	3	with	Nanocomposites	398:411	arg1	content					446:452	CNFs content	441:452	CNFs content	441:452	Nanocomposites with 0.5, 1.0 and 1.5wt% of CNFs content were prepared.
29410268	1	4	theme	thermoplastic	184:196	arg1	starch					198:203	thermoplastic starch	184:203	thermoplastic starch (TPS) from cornstarch flour	184:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	1	4	theme	thermoplastic	184:196	arg1	TPS					206:208	TPS	206:208	TPS	206:208	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	6	5	with	TPS	1041:1043	arg1	addition					1054:1061	the addition	1050:1061	the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	1050:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	6	6	theme	good	1082:1085	arg1	dispersion					1087:1096	its good dispersion	1078:1096	its good dispersion	1078:1096	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	5	7	theme	mechanical	617:626	arg1	properties					628:637	The mechanical properties	613:637	The mechanical properties	613:637	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	2	8	theme	cellulose	298:306	arg1	CNFs					320:323	CNFs	320:323	CNFs	320:323	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	2	8	theme	cellulose	298:306	arg1	nanofibers					308:317	cellulose nanofibers	298:317	cellulose nanofibers (CNFs)	298:324	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	11	9	theme	CNFs	1696:1699	arg1	addition					1684:1691	the addition	1680:1691	the addition of CNFs	1680:1699	The WA of the TPS/CNF composites moderately decreased with the addition of CNFs.
29410268	5	10	theme	neat	690:693	arg1	starch					709:714	neat thermoplastic starch	690:714	neat thermoplastic starch	690:714	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	2	11	theme	addition	286:293	arg1	effects					271:277	the effects	267:277	the effects	267:277	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	8	12	theme	TPS/CNF	1349:1355	arg1	nanocomposites					1357:1370	TPS/CNF nanocomposites	1349:1370	TPS/CNF nanocomposites	1349:1370	These findings confirmed the enhancement of mechanical properties of TPS/CNF nanocomposites.
29410268	7	13	theme	TPS/CNF	1213:1219	arg1	position					1201:1208	the tan δ peak position	1186:1208	the tan δ peak position	1186:1208	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	7	13	theme	TPS/CNF	1213:1219	arg1	modulus					1159:1165	The storage modulus	1147:1165	The storage modulus	1147:1165	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	7	13	theme	TPS/CNF	1213:1219	arg1	modulus					1173:1179	loss modulus	1168:1179	loss modulus	1168:1179	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	5	14	theme	starch	709:714	arg1	stability					677:685	thermal stability	669:685	thermal stability	669:685	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	14	theme	starch	709:714	arg1	properties					628:637	The mechanical properties	613:637	The mechanical properties	613:637	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	14	theme	starch	709:714	arg1	structure					654:662	morphological structure	640:662	morphological structure	640:662	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	1	15	theme	cornstarch	216:225	arg1	flour					227:231	cornstarch flour	216:231	cornstarch flour	216:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	7	16	theme	TPS	1268:1270	arg1	matrix					1272:1277	the TPS matrix	1264:1277	the TPS matrix	1264:1277	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	1	17	from	flour	227:231	arg1	starch					198:203	thermoplastic starch	184:203	thermoplastic starch (TPS) from cornstarch flour	184:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	1	17	from	flour	227:231	arg1	TPS					206:208	TPS	206:208	TPS	206:208	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	1	17	from	flour	227:231	arg1	preparation					169:179	the preparation	165:179	the preparation of thermoplastic starch (TPS) from cornstarch flour	165:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	2	18	from	effects	271:277	arg1	properties					371:380	TPS nanocomposite films properties	347:380	TPS nanocomposite films properties	347:380	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	6	19	theme	CNFs	1121:1124	arg1	dispersion					1087:1096	its good dispersion	1078:1096	its good dispersion	1078:1096	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	6	19	theme	CNFs	1121:1124	arg1	interaction					1106:1116	the interaction	1102:1116	the interaction of CNFs with the TPS matrix	1102:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	11	20	theme	TPS/CNF	1635:1641	arg1	composites					1643:1652	the TPS/CNF composites	1631:1652	the TPS/CNF composites	1631:1652	The WA of the TPS/CNF composites moderately decreased with the addition of CNFs.
29410268	7	21	with	comparison	1248:1257	arg1	matrix					1272:1277	the TPS matrix	1264:1277	the TPS matrix	1264:1277	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	9	22	theme	CNF	1429:1431	arg1	content					1433:1439	CNF content	1429:1439	CNF content	1429:1439	In addition, TGA and DSC results showed that increasing CNF content improves the thermal stability of TPS-based nanocomposites.
29410268	0	23	theme	green	85:89	arg1	nanocomposites					91:104	green nanocomposites	85:104	green nanocomposites: Extrusion processing	85:126	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	12	24	theme	cheap	1803:1807	arg1	nanocomposite					1828:1840	a natural, cheap and abundant green nanocomposite	1792:1840	a natural, cheap and abundant green nanocomposite	1792:1840	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	12	24	theme	cheap	1803:1807	arg1	CNFs					1786:1789	CNFs	1786:1789	CNFs	1786:1789	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	5	25	theme	differential	876:887	arg1	calorimetry					898:908	differential scanning calorimetry	876:908	differential scanning calorimetry (DSC)	876:914	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	25	theme	differential	876:887	arg1	DSC					911:913	DSC	911:913	DSC	911:913	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	12	26	theme	abundant	1813:1820	arg1	nanocomposite					1828:1840	a natural, cheap and abundant green nanocomposite	1792:1840	a natural, cheap and abundant green nanocomposite	1792:1840	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	12	26	theme	abundant	1813:1820	arg1	CNFs					1786:1789	CNFs	1786:1789	CNFs	1786:1789	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	9	27	theme	thermal	1454:1460	arg1	stability					1462:1470	the thermal stability	1450:1470	the thermal stability of TPS-based nanocomposites	1450:1498	In addition, TGA and DSC results showed that increasing CNF content improves the thermal stability of TPS-based nanocomposites.
29410268	2	28	theme	films	365:369	arg1	properties					371:380	TPS nanocomposite films properties	347:380	TPS nanocomposite films properties	347:380	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	5	29	theme	dynamic	798:804	arg1	DMTA					835:838	DMTA	835:838	DMTA	835:838	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	29	theme	dynamic	798:804	arg1	analysis					825:832	dynamic mechanical thermal analysis	798:832	dynamic mechanical thermal analysis (DMTA)	798:839	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	12	30	theme	natural	1794:1800	arg1	nanocomposite					1828:1840	a natural, cheap and abundant green nanocomposite	1792:1840	a natural, cheap and abundant green nanocomposite	1792:1840	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	12	30	theme	natural	1794:1800	arg1	CNFs					1786:1789	CNFs	1786:1789	CNFs	1786:1789	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	9	31	theme	nanocomposites	1485:1498	arg1	stability					1462:1470	the thermal stability	1450:1470	the thermal stability of TPS-based nanocomposites	1450:1498	In addition, TGA and DSC results showed that increasing CNF content improves the thermal stability of TPS-based nanocomposites.
29410268	6	32	theme	thermal	1003:1009	arg1	properties					1027:1036	the WA, thermal, and mechanical properties	995:1036	the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	995:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	4	33	theme	TPS/CNFs	511:518	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of starch, TPS and TPS/CNFs	469:518	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	10	34	theme	TPS/CNF	1594:1600	arg1	films					1602:1606	TPS/CNF films	1594:1606	TPS/CNF films	1594:1606	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	5	35	theme	thermal	817:823	arg1	DMTA					835:838	DMTA	835:838	DMTA	835:838	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	35	theme	thermal	817:823	arg1	analysis					825:832	dynamic mechanical thermal analysis	798:832	dynamic mechanical thermal analysis (DMTA)	798:839	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	12	36	theme	reinforcing	1765:1775	arg1	TPS					1777:1779	reinforcing TPS	1765:1779	reinforcing TPS	1765:1779	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	5	37	theme	absorption	926:935	arg1	test					942:945	water absorption (WA) test	920:945	water absorption (WA) test	920:945	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	6	38	theme	WA	999:1000	arg1	properties					1027:1036	the WA, thermal, and mechanical properties	995:1036	the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	995:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	0	39	theme	thermoplastic	36:48	arg1	starch					50:55	thermoplastic starch	36:55	thermoplastic starch	36:55	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	7	40	theme	δ	1194:1194	arg1	position					1201:1208	the tan δ peak position	1186:1208	the tan δ peak position	1186:1208	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	4	41	theme	starch	495:500	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of starch, TPS and TPS/CNFs	469:518	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	4	42	theme	TPS	503:505	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of starch, TPS and TPS/CNFs	469:518	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	9	43	dep	TGA	1386:1388	arg1	results					1398:1404	results	1398:1404	results	1398:1404	In addition, TGA and DSC results showed that increasing CNF content improves the thermal stability of TPS-based nanocomposites.
29410268	3	44	theme	content	446:452	arg1	%					436:436	0.5, 1.0 and 1.5wt%	418:436	0.5, 1.0 and 1.5wt% of CNFs content	418:452	Nanocomposites with 0.5, 1.0 and 1.5wt% of CNFs content were prepared.
29410268	3	44	theme	content	446:452	arg1	content					446:452	CNFs content	441:452	CNFs content	441:452	Nanocomposites with 0.5, 1.0 and 1.5wt% of CNFs content were prepared.
29410268	6	45	theme	TPS	1041:1043	arg1	properties					1027:1036	the WA, thermal, and mechanical properties	995:1036	the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	995:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	2	46	theme	TPS	347:349	arg1	properties					371:380	TPS nanocomposite films properties	347:380	TPS nanocomposite films properties	347:380	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	5	47	theme	thermogravimetric	842:858	arg1	TGA					870:872	TGA	870:872	TGA	870:872	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	47	theme	thermogravimetric	842:858	arg1	analysis					860:867	thermogravimetric analysis	842:867	thermogravimetric analysis (TGA)	842:873	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	1	48	theme	twin	239:242	arg1	extrusion					250:258	twin screw extrusion	239:258	twin screw extrusion	239:258	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	7	49	theme	loss	1168:1171	arg1	modulus					1173:1179	loss modulus	1168:1179	loss modulus	1168:1179	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	4	50	theme	Fourier-transform	542:558	arg1	spectroscopy					569:580	Fourier-transform infrared spectroscopy	542:580	Fourier-transform infrared spectroscopy (FT-IR)	542:588	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	4	50	theme	Fourier-transform	542:558	arg1	FT-IR					583:587	FT-IR	583:587	FT-IR	583:587	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	10	51	theme	SEM	1501:1503	arg1	images					1505:1510	SEM images	1501:1510	SEM images	1501:1510	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	5	52	theme	morphological	640:652	arg1	structure					654:662	morphological structure	640:662	morphological structure	640:662	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	10	53	theme	CNFs	1524:1527	arg1	dispersion					1529:1538	CNFs dispersion	1524:1538	CNFs dispersion within the TPS matrix	1524:1560	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	5	54	theme	electron	771:778	arg1	SEM					792:794	SEM	792:794	SEM	792:794	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	54	theme	electron	771:778	arg1	microscopy					780:789	scanning electron microscopy	762:789	scanning electron microscopy (SEM)	762:795	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	2	55	theme	nanofibers	308:317	arg1	addition					286:293	the addition	282:293	the addition of cellulose nanofibers (CNFs)	282:324	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	1	56	theme	starch	198:203	arg1	preparation					169:179	the preparation	165:179	the preparation of thermoplastic starch (TPS) from cornstarch flour	165:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	8	57	theme	nanocomposites	1357:1370	arg1	properties					1335:1344	mechanical properties	1324:1344	mechanical properties of TPS/CNF nanocomposites	1324:1370	These findings confirmed the enhancement of mechanical properties of TPS/CNF nanocomposites.
29410268	10	58	theme	TPS	1551:1553	arg1	matrix					1555:1560	the TPS matrix	1547:1560	the TPS matrix	1547:1560	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	5	59	theme	thermoplastic	695:707	arg1	starch					709:714	neat thermoplastic starch	690:714	neat thermoplastic starch	690:714	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	60	theme	scanning	889:896	arg1	calorimetry					898:908	differential scanning calorimetry	876:908	differential scanning calorimetry (DSC)	876:914	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	60	theme	scanning	889:896	arg1	DSC					911:913	DSC	911:913	DSC	911:913	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	6	61	theme	TPS	1135:1137	arg1	matrix					1139:1144	the TPS matrix	1131:1144	the TPS matrix	1131:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	5	62	theme	thermal	669:675	arg1	stability					677:685	thermal stability	669:685	thermal stability	669:685	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	8	63	theme	mechanical	1324:1333	arg1	properties					1335:1344	mechanical properties	1324:1344	mechanical properties of TPS/CNF nanocomposites	1324:1370	These findings confirmed the enhancement of mechanical properties of TPS/CNF nanocomposites.
29410268	4	64	theme	chemical	473:480	arg1	structure					482:490	The chemical structure	469:490	The chemical structure of starch, TPS and TPS/CNFs	469:518	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	11	65	theme	composites	1643:1652	arg1	WA					1625:1626	The WA	1621:1626	The WA of the TPS/CNF composites	1621:1652	The WA of the TPS/CNF composites moderately decreased with the addition of CNFs.
29410268	5	66	theme	scanning	762:769	arg1	SEM					792:794	SEM	792:794	SEM	792:794	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	66	theme	scanning	762:769	arg1	microscopy					780:789	scanning electron microscopy	762:789	scanning electron microscopy (SEM)	762:795	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	6	67	with	CNFs	1121:1124	arg1	matrix					1139:1144	the TPS matrix	1131:1144	the TPS matrix	1131:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	0	68	theme	Extrusion	107:115	arg1	processing					117:126	Extrusion processing	107:126	green nanocomposites: Extrusion processing	85:126	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	12	69	theme	green	1822:1826	arg1	nanocomposite					1828:1840	a natural, cheap and abundant green nanocomposite	1792:1840	a natural, cheap and abundant green nanocomposite	1792:1840	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	12	69	theme	green	1822:1826	arg1	CNFs					1786:1789	CNFs	1786:1789	CNFs	1786:1789	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	1	70	from	preparation	169:179	arg1	flour					227:231	cornstarch flour	216:231	cornstarch flour	216:231	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
29410268	5	71	theme	nanocomposites	726:739	arg1	stability					677:685	thermal stability	669:685	thermal stability	669:685	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	71	theme	nanocomposites	726:739	arg1	properties					628:637	The mechanical properties	613:637	The mechanical properties	613:637	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	71	theme	nanocomposites	726:739	arg1	structure					654:662	morphological structure	640:662	morphological structure	640:662	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	6	72	theme	mechanical	1016:1025	arg1	properties					1027:1036	the WA, thermal, and mechanical properties	995:1036	the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	995:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	0	73	dep	nanocomposites	91:104	arg1	processing					117:126	Extrusion processing	107:126	green nanocomposites: Extrusion processing	85:126	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	2	74	theme	nanocomposite	351:363	arg1	properties					371:380	TPS nanocomposite films properties	347:380	TPS nanocomposite films properties	347:380	Also, the effects of the addition of cellulose nanofibers (CNFs) as reinforcement, on TPS nanocomposite films properties were evaluated.
29410268	5	75	theme	mechanical	806:815	arg1	DMTA					835:838	DMTA	835:838	DMTA	835:838	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	75	theme	mechanical	806:815	arg1	analysis					825:832	dynamic mechanical thermal analysis	798:832	dynamic mechanical thermal analysis (DMTA)	798:839	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	12	76	theme	TPS	1777:1779	arg1	advantages					1751:1760	the advantages	1747:1760	the advantages of reinforcing TPS	1747:1779	Finally, the results obtained clearly showed the advantages of reinforcing TPS with CNFs, a natural, cheap and abundant green nanocomposite.
29410268	5	77	theme	water	920:924	arg1	WA					938:939	WA	938:939	WA	938:939	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	5	77	theme	water	920:924	arg1	absorption					926:935	water absorption	920:935	water absorption (WA) test	920:945	The mechanical properties, morphological structure, and thermal stability of neat thermoplastic starch and their nanocomposites were investigated by scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and water absorption (WA) test.
29410268	7	78	theme	peak	1196:1199	arg1	position					1201:1208	the tan δ peak position	1186:1208	the tan δ peak position	1186:1208	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	3	79	theme	CNFs	441:444	arg1	content					446:452	CNFs content	441:452	CNFs content	441:452	Nanocomposites with 0.5, 1.0 and 1.5wt% of CNFs content were prepared.
29410268	0	80	theme	starch	50:55	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	0	80	theme	starch	50:55	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	7	81	theme	tan	1190:1192	arg1	position					1201:1208	the tan δ peak position	1186:1208	the tan δ peak position	1186:1208	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	0	82	theme	cellulose	61:69	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	0	82	theme	cellulose	61:69	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites: Extrusion processing.
29410268	7	83	dep	nanocomposites	1221:1234	arg1	improved					1236:1243	improved	1236:1243	nanocomposites improved in comparison with the TPS matrix	1221:1277	The storage modulus, loss modulus, and the tan δ peak position of TPS/CNF nanocomposites improved in comparison with the TPS matrix.
29410268	6	84	theme	CNFs	1066:1069	arg1	addition					1054:1061	the addition	1050:1061	the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix	1050:1144	Results showed that it was possible to improve the WA, thermal, and mechanical properties of TPS with the addition of CNFs due to its good dispersion and the interaction of CNFs with the TPS matrix.
29410268	10	85	theme	films	1602:1606	arg1	smooth					1613:1618	smooth	1613:1618	smooth	1613:1618	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	10	85	theme	films	1602:1606	arg1	surfaces					1582:1589	the surfaces	1578:1589	the surfaces of TPS/CNF films	1578:1606	SEM images showed that CNFs dispersion within the TPS matrix was uniform and the surfaces of TPS/CNF films were smooth.
29410268	4	86	theme	X-ray	594:598	arg1	diffraction					600:610	X-ray diffraction	594:610	X-ray diffraction	594:610	The chemical structure of starch, TPS and TPS/CNFs were characterized by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction.
29410268	8	87	theme	properties	1335:1344	arg1	enhancement					1309:1319	the enhancement	1305:1319	the enhancement of mechanical properties of TPS/CNF nanocomposites	1305:1370	These findings confirmed the enhancement of mechanical properties of TPS/CNF nanocomposites.
29410268	1	88	theme	screw	244:248	arg1	extrusion					250:258	twin screw extrusion	239:258	twin screw extrusion	239:258	This research was aimed at studying the preparation of thermoplastic starch (TPS) from cornstarch flour using twin screw extrusion.
31040318	0	0	theme	sanguinis	88:96	arg1	biofilms					37:44	dual-species biofilms	24:44	dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis	24:96	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.
31040318	8	1	theme	extracellular	999:1011	arg1	polysaccharide					1013:1026	extracellular polysaccharide	999:1026	extracellular polysaccharide (EPS) synthesis	999:1042	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	1	theme	extracellular	999:1011	arg1	EPS					1029:1031	EPS	1029:1031	EPS	1029:1031	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	6	2	theme	study	702:706	arg1	aim					683:685	The aim	679:685	The aim of the current study	679:706	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	7	3	theme	biofilms	867:874	arg1	biomass					876:882	biofilms biomass	867:882	biofilms biomass	867:882	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	7	3	theme	biofilms	867:874	arg1	activity					895:902	metabolic activity	885:902	metabolic activity	885:902	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	5	4	theme	S.	580:581	arg1	sanguinis					583:591	S. sanguinis	580:591	S. sanguinis	580:591	However, whether D-Cys could affect S. sanguinis and the interspecies relationship between S. mutans and S. sanguinis remains unknown.
31040318	10	5	theme	biofilms	1233:1240	arg1	formation					1242:1250	biofilms formation	1233:1250	biofilms formation	1233:1250	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	0	6	from	Effect	0:5	arg1	biofilms					37:44	dual-species biofilms	24:44	dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis	24:96	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.
31040318	6	7	theme	dual-species	782:793	arg1	biofilms					795:802	dual-species biofilms	782:802	dual-species biofilms formed by S. mutans and S. sanguinis	782:839	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	8	8	theme	polysaccharide	1013:1026	arg1	biofilms					946:953	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	8	theme	polysaccharide	1013:1026	arg1	synthesis					1034:1042	extracellular polysaccharide (EPS) synthesis	999:1042	extracellular polysaccharide (EPS) synthesis	999:1042	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	9	9	theme	acid	1167:1170	arg1	production					1172:1181	lactic acid production	1160:1181	lactic acid production	1160:1181	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	2	10	from	bacteria	223:230	arg1	surface					242:248	dental surface	235:248	dental surface	235:248	It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface.
31040318	6	11	theme	current	694:700	arg1	study					702:706	the current study	690:706	the current study	690:706	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	5	12	theme	interspecies	601:612	arg1	relationship					614:625	the interspecies relationship	597:625	the interspecies relationship between S. mutans and S. sanguinis	597:660	However, whether D-Cys could affect S. sanguinis and the interspecies relationship between S. mutans and S. sanguinis remains unknown.
31040318	10	13	theme	D-Cys	1345:1349	arg1	treatment					1351:1359	D-Cys treatment	1345:1359	D-Cys treatment	1345:1359	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	11	14	theme	dual-species	1448:1459	arg1	biofilms					1461:1468	dual-species biofilms	1448:1468	dual-species biofilms formed by S. mutans and S. sanguinis	1448:1505	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	11	15	theme	D-Cys	1536:1540	arg1	potential					1523:1531	the potential	1519:1531	the potential of D-Cys in clinical application for caries prevention and treatment	1519:1600	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	9	16	theme	dual-species	1186:1197	arg1	p < 0.05					1209:1216	p < 0.05	1209:1216	p < 0.05	1209:1216	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	9	16	theme	dual-species	1186:1197	arg1	biofilms					1199:1206	dual-species biofilms	1186:1206	dual-species biofilms (p < 0.05)	1186:1217	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	8	17	theme	bacteria	989:996	arg1	biofilms					946:953	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	17	theme	bacteria	989:996	arg1	synthesis					1034:1042	extracellular polysaccharide (EPS) synthesis	999:1042	extracellular polysaccharide (EPS) synthesis	999:1042	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	17	theme	bacteria	989:996	arg1	structure					955:963	structure	955:963	structure	955:963	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	17	theme	bacteria	989:996	arg1	ratio					970:974	the ratio	966:974	the ratio of live/dead bacteria	966:996	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	17	theme	bacteria	989:996	arg1	composition					1058:1068	bacterial composition	1048:1068	bacterial composition	1048:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	1	18	theme	Dental	99:104	arg1	caries					106:111	Dental caries	99:111	Dental caries	99:111	Dental caries is a highly prevalent disease worldwide.
31040318	1	18	theme	Dental	99:104	arg1	worldwide					143:151	a highly prevalent disease worldwide	116:151	a highly prevalent disease worldwide	116:151	Dental caries is a highly prevalent disease worldwide.
31040318	10	19	theme	dual-species	1284:1295	arg1	biofilms					1297:1304	dual-species biofilms	1284:1304	dual-species biofilms	1284:1304	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	9	20	theme	biofilms	1199:1206	arg1	production					1172:1181	lactic acid production	1160:1181	lactic acid production	1160:1181	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	9	20	theme	biofilms	1199:1206	arg1	activity					1147:1154	metabolic activity	1137:1154	metabolic activity	1137:1154	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	7	21	theme	lactate	905:911	arg1	production					913:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	10	22	from	formation	1242:1250	arg1	biofilms					1297:1304	dual-species biofilms	1284:1304	dual-species biofilms	1284:1304	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	6	23	theme	biofilms	795:802	arg1	growth					754:759	growth	754:759	growth	754:759	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	6	23	theme	biofilms	795:802	arg1	cariogenicity					765:777	cariogenicity	765:777	cariogenicity	765:777	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	0	24	theme	D-cysteine	10:19	arg1	Effect					0:5	Effect	0:5	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.	0:97	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.
31040318	8	25	theme	bacterial	1048:1056	arg1	biofilms					946:953	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	25	theme	bacterial	1048:1056	arg1	composition					1058:1068	bacterial composition	1048:1068	bacterial composition	1048:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	2	26	theme	cariogenic	174:183	arg1	biofilms					185:192	the cariogenic biofilms	170:192	the cariogenic biofilms composed of multiple dynamic bacteria on dental surface	170:248	It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface.
31040318	11	27	theme	cariogenic	1424:1433	arg1	virulence					1435:1443	cariogenic virulence	1424:1443	cariogenic virulence	1424:1443	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	10	28	theme	mutans	1274:1279	arg1	proportion					1257:1266	the proportion	1253:1266	the proportion of S. mutans in dual-species biofilms	1253:1304	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	10	28	theme	mutans	1274:1279	arg1	synthesis					1315:1323	EPS synthesis	1311:1323	EPS synthesis	1311:1323	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	10	28	theme	mutans	1274:1279	arg1	formation					1242:1250	biofilms formation	1233:1250	biofilms formation	1233:1250	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	10	29	from	synthesis	1315:1323	arg1	biofilms					1297:1304	dual-species biofilms	1284:1304	dual-species biofilms	1284:1304	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	0	30	theme	mutans	63:68	arg1	biofilms					37:44	dual-species biofilms	24:44	dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis	24:96	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.
31040318	2	31	theme	dental	235:240	arg1	surface					242:248	dental surface	235:248	dental surface	235:248	It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface.
31040318	8	32	dep	biofilms	946:953	arg1	biofilms					946:953	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition	942:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	32	dep	biofilms	946:953	arg1	synthesis					1034:1042	extracellular polysaccharide (EPS) synthesis	999:1042	extracellular polysaccharide (EPS) synthesis	999:1042	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	32	dep	biofilms	946:953	arg1	structure					955:963	structure	955:963	structure	955:963	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	32	dep	biofilms	946:953	arg1	ratio					970:974	the ratio	966:974	the ratio of live/dead bacteria	966:996	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	8	32	dep	biofilms	946:953	arg1	composition					1058:1068	bacterial composition	1048:1068	bacterial composition	1048:1068	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	11	33	from	potential	1523:1531	arg1	application					1554:1564	clinical application	1545:1564	clinical application for caries prevention and treatment	1545:1600	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	0	34	theme	dual-species	24:35	arg1	biofilms					37:44	dual-species biofilms	24:44	dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis	24:96	Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.
31040318	4	35	theme	caries	474:479	arg1	mutans					419:424	S. mutans	416:424	S. mutans	416:424	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	4	35	theme	caries	474:479	arg1	microorganism					450:462	the major causative microorganism	430:462	the major causative microorganism of dental caries	430:479	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	6	36	theme	D-Cys	741:745	arg1	effect					731:736	the effect	727:736	the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis	727:839	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	4	37	theme	free	519:522	arg1	D-Cys					536:540	D-Cys	536:540	D-Cys	536:540	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	4	37	theme	free	519:522	arg1	D-cysteine					524:533	free D-cysteine	519:533	free D-cysteine (D-Cys)	519:541	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	4	38	theme	dental	467:472	arg1	caries					474:479	dental caries	467:479	dental caries	467:479	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	11	39	theme	caries	1570:1575	arg1	prevention					1577:1586	caries prevention	1570:1586	caries prevention	1570:1586	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	6	40	from	effect	731:736	arg1	growth					754:759	growth	754:759	growth	754:759	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	6	40	from	effect	731:736	arg1	cariogenicity					765:777	cariogenicity	765:777	cariogenicity	765:777	The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis.
31040318	8	41	theme	dual-species	1077:1088	arg1	biofilms					1090:1097	the dual-species biofilms	1073:1097	the dual-species biofilms	1073:1097	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	2	42	theme	multiple	206:213	arg1	bacteria					223:230	multiple dynamic bacteria	206:230	multiple dynamic bacteria on dental surface	206:248	It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface.
31040318	2	43	theme	dynamic	215:221	arg1	bacteria					223:230	multiple dynamic bacteria	206:230	multiple dynamic bacteria on dental surface	206:248	It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface.
31040318	11	44	theme	clinical	1545:1552	arg1	application					1554:1564	clinical application	1545:1564	clinical application for caries prevention and treatment	1545:1600	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	7	45	theme	metabolic	885:893	arg1	biomass					876:882	biofilms biomass	867:882	biofilms biomass	867:882	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	7	45	theme	metabolic	885:893	arg1	activity					895:902	metabolic activity	885:902	metabolic activity	885:902	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	8	46	theme	live/dead	979:987	arg1	bacteria					989:996	live/dead bacteria	979:996	live/dead bacteria	979:996	We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms.
31040318	3	47	theme	resident	304:311	arg1	sanguinis					290:298	Streptococcus sanguinis	276:298	Streptococcus sanguinis	276:298	Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species.
31040318	3	47	theme	resident	304:311	arg1	members					313:319	resident members	304:319	resident members within the biofilms	304:339	Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species.
31040318	3	47	theme	resident	304:311	arg1	mutans					265:270	Streptococcus mutans	251:270	Streptococcus mutans	251:270	Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species.
31040318	9	48	theme	lactic	1160:1165	arg1	production					1172:1181	lactic acid production	1160:1181	lactic acid production	1160:1181	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	1	49	theme	prevalent	125:133	arg1	caries					106:111	Dental caries	99:111	Dental caries	99:111	Dental caries is a highly prevalent disease worldwide.
31040318	1	49	theme	prevalent	125:133	arg1	worldwide					143:151	a highly prevalent disease worldwide	116:151	a highly prevalent disease worldwide	116:151	Dental caries is a highly prevalent disease worldwide.
31040318	9	50	dep	activity	1147:1154	arg1	the					1133:1135	the	1133:1135	the	1133:1135	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	4	51	theme	causative	440:448	arg1	mutans					419:424	S. mutans	416:424	S. mutans	416:424	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	4	51	theme	causative	440:448	arg1	microorganism					450:462	the major causative microorganism	430:462	the major causative microorganism of dental caries	430:479	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	10	52	theme	EPS	1311:1313	arg1	synthesis					1315:1323	EPS synthesis	1311:1323	EPS synthesis	1311:1323	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	1	53	theme	disease	135:141	arg1	caries					106:111	Dental caries	99:111	Dental caries	99:111	Dental caries is a highly prevalent disease worldwide.
31040318	1	53	theme	disease	135:141	arg1	worldwide					143:151	a highly prevalent disease worldwide	116:151	a highly prevalent disease worldwide	116:151	Dental caries is a highly prevalent disease worldwide.
31040318	7	54	theme	biomass	876:882	arg1	production					913:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31040318	10	55	from	proportion	1257:1266	arg1	biofilms					1297:1304	dual-species biofilms	1284:1304	dual-species biofilms	1284:1304	In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment.
31040318	4	56	theme	major	434:438	arg1	mutans					419:424	S. mutans	416:424	S. mutans	416:424	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	4	56	theme	major	434:438	arg1	microorganism					450:462	the major causative microorganism	430:462	the major causative microorganism of dental caries	430:479	S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys).
31040318	11	57	theme	biofilms	1461:1468	arg1	growth					1413:1418	growth	1413:1418	growth	1413:1418	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	11	57	theme	biofilms	1461:1468	arg1	virulence					1435:1443	cariogenic virulence	1424:1443	cariogenic virulence	1424:1443	The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.
31040318	3	58	theme	antagonistic	348:359	arg1	relationship					361:372	an antagonistic relationship	345:372	an antagonistic relationship	345:372	Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species.
31040318	9	59	theme	metabolic	1137:1145	arg1	activity					1147:1154	metabolic activity	1137:1154	metabolic activity	1137:1154	We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05).
31040318	7	60	theme	dual-species	854:865	arg1	production					913:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	dual-species biofilms biomass, metabolic activity, lactate production	854:922	We measured dual-species biofilms biomass, metabolic activity, lactate production.
31255621	0	0	theme	emulsion	69:76	arg1	approach					78:85	the Pickering emulsion approach	55:85	the Pickering emulsion approach	55:85	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	0	1	theme	Pickering	59:67	arg1	approach					78:85	the Pickering emulsion approach	55:85	the Pickering emulsion approach	55:85	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	4	2	theme	composites	645:654	arg1	transition					627:636	the transition	623:636	the transition of the composites from fluid to solid-like behavior	623:688	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	6	3	theme	storage	864:870	arg1	modulus					872:878	The storage modulus	860:878	The storage modulus of the composites	860:896	The storage modulus of the composites increased throughout the entire temperature range of testing.
31255621	4	4	from	Changes	537:543	arg1	characteristics					564:578	the rheological characteristics	548:578	the rheological characteristics of the composites	548:596	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	6	5	theme	testing	951:957	arg1	range					942:946	the entire temperature range	919:946	the entire temperature range of testing	919:957	The storage modulus of the composites increased throughout the entire temperature range of testing.
31255621	1	6	theme	nanocellulose	147:159	arg1	use					140:142	The use	136:142	The use of nanocellulose	136:159	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	1	6	theme	nanocellulose	147:159	arg1	method					178:183	an attractive method	164:183	an attractive method to improve the characteristics of biodegradable polymers	164:240	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	0	7	theme	Rheological	88:98	arg1	properties					124:133	thermal and mechanical properties	101:133	thermal and mechanical properties	101:133	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	5	8	theme	%	736:736	arg1	CNC					738:740	5 wt% CNC	732:740	5 wt% CNC	732:740	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	3	9	theme	rheological	480:490	arg1	measurements					523:534	rheological, thermal as well as mechanical measurements	480:534	rheological, thermal as well as mechanical measurements	480:534	The PLA/CNC composites prepared were studied by rheological, thermal as well as mechanical measurements.
31255621	8	10	theme	PLA	1164:1166	arg1	properties					1150:1159	most properties	1145:1159	most properties of PLA	1145:1166	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	5	11	theme	5 wt	732:735	arg1	CNC					738:740	5 wt% CNC	732:740	5 wt% CNC	732:740	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	8	12	theme	emulsion	1049:1056	arg1	approach					1058:1065	the Pickering emulsion approach	1035:1065	the Pickering emulsion approach	1035:1065	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	4	13	theme	high	693:696	arg1	temperatures					698:709	high temperatures	693:709	high temperatures	693:709	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	5	14	theme	crystallization	828:842	arg1	temperature					813:823	the onset temperature	803:823	the onset temperature of crystallization	803:842	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	8	15	theme	Pickering	1039:1047	arg1	approach					1058:1065	the Pickering emulsion approach	1035:1065	the Pickering emulsion approach	1035:1065	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	3	16	theme	mechanical	512:521	arg1	measurements					523:534	rheological, thermal as well as mechanical measurements	480:534	rheological, thermal as well as mechanical measurements	480:534	The PLA/CNC composites prepared were studied by rheological, thermal as well as mechanical measurements.
31255621	1	17	theme	attractive	167:176	arg1	use					140:142	The use	136:142	The use of nanocellulose	136:159	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	1	17	theme	attractive	167:176	arg1	method					178:183	an attractive method	164:183	an attractive method to improve the characteristics of biodegradable polymers	164:240	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	5	18	theme	PLA	772:774	arg1	crystallinity					755:767	the crystallinity	751:767	the crystallinity of PLA	751:774	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	8	19	theme	most	1145:1148	arg1	properties					1150:1159	most properties	1145:1159	most properties of PLA	1145:1166	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	4	20	theme	composites	587:596	arg1	characteristics					564:578	the rheological characteristics	548:578	the rheological characteristics of the composites	548:596	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	0	21	theme	lactic	5:10	arg1	Poly					0:3	Poly	0:3	Poly(lactic acid)	0:16	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	0	21	theme	lactic	5:10	arg1	acid					12:15	lactic acid	5:15	lactic acid	5:15	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	6	22	theme	temperature	930:940	arg1	range					942:946	the entire temperature range	919:946	the entire temperature range of testing	919:957	The storage modulus of the composites increased throughout the entire temperature range of testing.
31255621	0	23	theme	thermal	101:107	arg1	properties					124:133	thermal and mechanical properties	101:133	thermal and mechanical properties	101:133	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	2	24	theme	lactic	376:381	arg1	poly					371:374	poly	371:374	poly(lactic acid) (PLA)	371:393	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	2	24	theme	lactic	376:381	arg1	acid					383:386	lactic acid	376:386	lactic acid	376:386	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	2	25	theme	emulsion	413:420	arg1	approach					422:429	the Pickering emulsion approach	399:429	the Pickering emulsion approach	399:429	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	3	26	theme	PLA/CNC	436:442	arg1	composites					444:453	The PLA/CNC composites	432:453	The PLA/CNC composites prepared	432:462	The PLA/CNC composites prepared were studied by rheological, thermal as well as mechanical measurements.
31255621	6	27	theme	entire	923:928	arg1	range					942:946	the entire temperature range	919:946	the entire temperature range of testing	919:957	The storage modulus of the composites increased throughout the entire temperature range of testing.
31255621	2	28	theme	Pickering	403:411	arg1	approach					422:429	the Pickering emulsion approach	399:429	the Pickering emulsion approach	399:429	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	1	29	theme	uneven	281:286	arg1	dispersion					288:297	uneven dispersion	281:297	uneven dispersion	281:297	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	0	30	theme	mechanical	113:122	arg1	properties					124:133	thermal and mechanical properties	101:133	thermal and mechanical properties	101:133	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	5	31	theme	onset	807:811	arg1	temperature					813:823	the onset temperature	803:823	the onset temperature of crystallization	803:842	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	8	32	theme	CNC	1094:1096	arg1	dispersion					1080:1089	the dispersion	1076:1089	the dispersion of CNC	1076:1096	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	2	33	theme	cellulose	314:322	arg1	CNCs					338:341	CNCs	338:341	CNCs	338:341	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	2	33	theme	cellulose	314:322	arg1	nanocrystals					324:335	cellulose nanocrystals	314:335	cellulose nanocrystals (CNCs)	314:342	In this work, cellulose nanocrystals (CNCs) were evenly dispersed into poly(lactic acid) (PLA) via the Pickering emulsion approach.
31255621	0	34	theme	nanocrystal	28:38	arg1	composites					40:49	nanocrystal composites	28:49	nanocrystal composites	28:49	Poly(lactic acid)/cellulose nanocrystal composites via the Pickering emulsion approach: Rheological, thermal and mechanical properties.
31255621	3	35	dep	rheological	480:490	arg1	thermal					493:499	thermal	493:499	thermal	493:499	The PLA/CNC composites prepared were studied by rheological, thermal as well as mechanical measurements.
31255621	6	36	theme	composites	887:896	arg1	modulus					872:878	The storage modulus	860:878	The storage modulus of the composites	860:896	The storage modulus of the composites increased throughout the entire temperature range of testing.
31255621	4	37	theme	rheological	552:562	arg1	characteristics					564:578	the rheological characteristics	548:578	the rheological characteristics of the composites	548:596	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	7	38	theme	Flexural	960:967	arg1	modulus					969:975	Flexural modulus	960:975	Flexural modulus	960:975	Flexural modulus was improved considerably.
31255621	4	39	theme	solid-like	670:679	arg1	behavior					681:688	solid-like behavior	670:688	solid-like behavior	670:688	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	4	40	from	fluid	661:665	arg1	transition					627:636	the transition	623:636	the transition of the composites from fluid to solid-like behavior	623:688	Changes in the rheological characteristics of the composites showed that CNC promoted the transition of the composites from fluid to solid-like behavior at high temperatures.
31255621	1	41	theme	biodegradable	219:231	arg1	polymers					233:240	biodegradable polymers	219:240	biodegradable polymers	219:240	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31255621	8	42	theme	PLA	1105:1107	arg1	matrix					1109:1114	the PLA matrix	1101:1114	the PLA matrix	1101:1114	All the results indicated that the Pickering emulsion approach improved the dispersion of CNC in the PLA matrix and CNC improved efficiently most properties of PLA.
31255621	5	43	theme	CNC	738:740	arg1	introduction					716:727	The introduction	712:727	The introduction of 5 wt% CNC	712:740	The introduction of 5 wt% CNC improved the crystallinity of PLA considerably and increased the onset temperature of crystallization by about 10 °C.
31255621	1	44	theme	polymers	233:240	arg1	characteristics					200:214	the characteristics	196:214	the characteristics of biodegradable polymers	196:240	The use of nanocellulose is an attractive method to improve the characteristics of biodegradable polymers, but its effects are often affected by uneven dispersion.
31401281	0	0	theme	aggressive	76:85	arg1	media					96:100	aggressive chloride media	76:100	aggressive chloride media	76:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	4	1	theme	sulfide	430:436	arg1	nanoparticles					438:450	cobalt and tin sulfide nanoparticles	415:450	cobalt and tin sulfide nanoparticles	415:450	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	7	2	from	effective	1043:1051	arg1	inhibition					1066:1075	corrosion inhibition	1056:1075	corrosion inhibition of mild steel	1056:1089	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	1	3	theme	bio-polymers	144:155	arg1	bio-polymers					144:155	the important natural bio-polymers	122:155	the important natural bio-polymers	122:155	Chitosan is one of the important natural bio-polymers.
31401281	1	3	theme	bio-polymers	144:155	arg1	one					115:117	one	115:117	one	115:117	Chitosan is one of the important natural bio-polymers.
31401281	6	4	theme	polarization	854:865	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	4	theme	polarization	854:865	arg1	techniques					962:971	surface analyzing techniques	944:971	surface analyzing techniques	944:971	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	4	theme	polarization	854:865	arg1	TPC					875:877	TPC	875:877	TPC	875:877	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	4	theme	polarization	854:865	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	5	theme	Tafel	848:852	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	5	theme	Tafel	848:852	arg1	techniques					962:971	surface analyzing techniques	944:971	surface analyzing techniques	944:971	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	5	theme	Tafel	848:852	arg1	TPC					875:877	TPC	875:877	TPC	875:877	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	5	theme	Tafel	848:852	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	7	6	theme	mild	1080:1083	arg1	steel					1085:1089	mild steel	1080:1089	mild steel	1080:1089	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	2	7	from	molecules	291:299	arg1	acid					304:307	acid	304:307	acid	304:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	6	8	theme	Corrosion	789:797	arg1	behavior					799:806	Corrosion behavior	789:806	Corrosion behavior of the composites	789:824	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	7	9	theme	corrosion	1056:1064	arg1	inhibition					1066:1075	corrosion inhibition	1056:1075	corrosion inhibition of mild steel	1056:1089	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	2	10	from	degradation	272:282	arg1	acid					304:307	acid	304:307	acid	304:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	9	11	theme	inhibition	1355:1364	arg1	reason					1345:1350	main reason	1340:1350	main reason of inhibition	1340:1364	Isotherm study suggests that adsorption of chitosan/composites over MS is main reason of inhibition and can be best defined with Frumkin isotherm.
31401281	0	12	theme	chloride	87:94	arg1	media					96:100	aggressive chloride media	76:100	aggressive chloride media	76:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	4	13	theme	cobalt	415:420	arg1	nanoparticles					438:450	cobalt and tin sulfide nanoparticles	415:450	cobalt and tin sulfide nanoparticles	415:450	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	2	14	from	acid	304:307	arg1	degradation					272:282	rapid degradation	266:282	rapid degradation of its molecules in acid	266:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	4	15	from	temperature	597:607	arg1	HCl					585:587	1 M HCl	581:587	1 M HCl at room temperature	581:607	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	4	16	theme	corrosion	538:546	arg1	protection					548:557	corrosion protection	538:557	corrosion protection of mild steel (MS)	538:576	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	5	17	theme	FTIR	679:682	arg1	spectroscopy					684:695	FTIR spectroscopy	679:695	FTIR spectroscopy	679:695	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	0	18	from	protection	48:57	arg1	media					96:100	aggressive chloride media	76:100	aggressive chloride media	76:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	2	19	theme	direct	247:252	arg1	form					254:257	direct form	247:257	direct form due to rapid degradation of its molecules in acid	247:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	4	20	theme	tin	426:428	arg1	sulfide					430:436	tin sulfide	426:436	tin sulfide	426:436	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	6	21	theme	electrochemical	893:907	arg1	SSEIS					933:937	SSEIS	933:937	SSEIS	933:937	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	21	theme	electrochemical	893:907	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	21	theme	electrochemical	893:907	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	3	22	theme	chitosan	388:395	arg1	efficiency					374:383	protection efficiency	363:383	protection efficiency of chitosan	363:395	Hence, it is necessary to use some means to increase protection efficiency of chitosan.
31401281	6	23	theme	composites	815:824	arg1	behavior					799:806	Corrosion behavior	789:806	Corrosion behavior of the composites	789:824	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	3	24	theme	protection	363:372	arg1	efficiency					374:383	protection efficiency	363:383	protection efficiency of chitosan	363:395	Hence, it is necessary to use some means to increase protection efficiency of chitosan.
31401281	4	25	used	used	456:459	arg2	nanoparticles					438:450	cobalt and tin sulfide nanoparticles	415:450	cobalt and tin sulfide nanoparticles	415:450	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	6	26	theme	impedance	909:917	arg1	SSEIS					933:937	SSEIS	933:937	SSEIS	933:937	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	26	theme	impedance	909:917	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	26	theme	impedance	909:917	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	7	27	theme	chitosan-SnS2	1096:1108	arg1	nanocomposite					1110:1122	chitosan-SnS2 nanocomposite	1096:1122	chitosan-SnS2 nanocomposite	1096:1122	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	4	28	theme	room	592:595	arg1	temperature					597:607	room temperature	592:607	room temperature	592:607	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	5	29	theme	nanocomposites	773:786	arg1	formation					760:768	successful formation	749:768	successful formation of nanocomposites	749:786	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	4	30	theme	chitosan-SnS2	495:507	arg1	nanocomposites					509:522	chitosan-cobalt and chitosan-SnS2 nanocomposites	475:522	chitosan-cobalt and chitosan-SnS2 nanocomposites	475:522	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	7	31	theme	chitosan-cobalt	1005:1019	arg1	nanocomposite					1021:1033	chitosan-cobalt nanocomposite	1005:1033	chitosan-cobalt nanocomposite	1005:1033	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	2	32	theme	rapid	266:270	arg1	degradation					272:282	rapid degradation	266:282	rapid degradation of its molecules in acid	266:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	2	33	used	used	201:204	arg2	it					188:189	it	188:189	it	188:189	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	0	34	theme	new	15:17	arg1	nanocomposites					19:32	new nanocomposites	15:32	new nanocomposites for corrosion protection of mild steel in aggressive chloride media	15:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	6	35	theme	sine	888:891	arg1	SSEIS					933:937	SSEIS	933:937	SSEIS	933:937	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	35	theme	sine	888:891	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	35	theme	sine	888:891	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	10	36	theme	chitosan	1427:1434	arg1	Adsorption					1413:1422	Adsorption	1413:1422	Adsorption of chitosan and chitosan-nanocomposites over MS	1413:1470	Adsorption of chitosan and chitosan-nanocomposites over MS is also evident by SEM and AFM.
31401281	2	37	theme	green	175:179	arg1	value					181:185	its good green value	166:185	its good green value	166:185	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	9	38	theme	Frumkin	1395:1401	arg1	isotherm					1403:1410	Frumkin isotherm	1395:1410	Frumkin isotherm	1395:1410	Isotherm study suggests that adsorption of chitosan/composites over MS is main reason of inhibition and can be best defined with Frumkin isotherm.
31401281	0	39	theme	corrosion	38:46	arg1	protection					48:57	corrosion protection	38:57	corrosion protection of mild steel in aggressive chloride media	38:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	6	40	theme	single	881:886	arg1	SSEIS					933:937	SSEIS	933:937	SSEIS	933:937	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	40	theme	single	881:886	arg1	spectroscopy					919:930	single sine electrochemical impedance spectroscopy	881:930	single sine electrochemical impedance spectroscopy (SSEIS)	881:938	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	40	theme	single	881:886	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	2	41	theme	good	170:173	arg1	value					181:185	its good green value	166:185	its good green value	166:185	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	9	42	theme	chitosan/composites	1309:1327	arg1	adsorption					1295:1304	adsorption	1295:1304	adsorption of chitosan/composites over MS	1295:1335	Isotherm study suggests that adsorption of chitosan/composites over MS is main reason of inhibition and can be best defined with Frumkin isotherm.
31401281	4	43	theme	1 M	581:583	arg1	HCl					585:587	1 M HCl	581:587	1 M HCl at room temperature	581:607	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	5	44	theme	EDAX	715:718	arg1	techniques					720:729	EDAX techniques	715:729	EDAX techniques	715:729	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	5	45	theme	UV-visible	654:663	arg1	spectroscopy					665:676	UV-visible spectroscopy	654:676	UV-visible spectroscopy	654:676	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	9	46	theme	Isotherm	1266:1273	arg1	study					1275:1279	Isotherm study	1266:1279	Isotherm study	1266:1279	Isotherm study suggests that adsorption of chitosan/composites over MS is main reason of inhibition and can be best defined with Frumkin isotherm.
31401281	8	47	theme	chitosan-SnS2	1233:1245	arg1	%					1251:1251	>80%	1248:1251	>80%	1248:1251	Alone chitosan provide only 77% inhibition to mild steel, while it increases for chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites.
31401281	8	47	theme	chitosan-SnS2	1233:1245	arg1	composites					1254:1263	chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites	1206:1263	composites	1254:1263	Alone chitosan provide only 77% inhibition to mild steel, while it increases for chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites.
31401281	7	48	theme	steel	1085:1089	arg1	inhibition					1066:1075	corrosion inhibition	1056:1075	corrosion inhibition of mild steel	1056:1089	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	7	49	from	inhibition	1066:1075	arg1	effective					1043:1051	effective	1043:1051	effective	1043:1051	The results have declared that chitosan-cobalt nanocomposite is more effective in corrosion inhibition of mild steel than chitosan-SnS2 nanocomposite.
31401281	8	50	dep	inhibition	1157:1166	arg1	%					1155:1155	%	1155:1155	%	1155:1155	Alone chitosan provide only 77% inhibition to mild steel, while it increases for chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites.
31401281	4	51	theme	present	401:407	arg1	work					409:412	present work	401:412	present work	401:412	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	5	52	theme	prepared	614:621	arg1	composites					623:632	The prepared composites	610:632	The prepared composites	610:632	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	8	53	dep	%	1155:1155	arg1	77					1153:1154	77	1153:1154	77	1153:1154	Alone chitosan provide only 77% inhibition to mild steel, while it increases for chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites.
31401281	8	54	theme	mild	1171:1174	arg1	steel					1176:1180	mild steel	1171:1180	mild steel	1171:1180	Alone chitosan provide only 77% inhibition to mild steel, while it increases for chitosan‑cobalt (>95%) and chitosan-SnS2 (>80%) composites.
31401281	4	55	theme	chitosan-cobalt	475:489	arg1	nanocomposites					509:522	chitosan-cobalt and chitosan-SnS2 nanocomposites	475:522	chitosan-cobalt and chitosan-SnS2 nanocomposites	475:522	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	4	56	used	used	529:532	arg2	nanoparticles					438:450	cobalt and tin sulfide nanoparticles	415:450	cobalt and tin sulfide nanoparticles	415:450	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	2	57	theme	molecules	291:299	arg1	degradation					272:282	rapid degradation	266:282	rapid degradation of its molecules in acid	266:307	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	4	58	theme	mild	562:565	arg1	MS					574:575	MS	574:575	MS	574:575	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	4	58	theme	mild	562:565	arg1	steel					567:571	mild steel	562:571	mild steel (MS)	562:576	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
31401281	10	59	theme	chitosan-nanocomposites	1440:1462	arg1	Adsorption					1413:1422	Adsorption	1413:1422	Adsorption of chitosan and chitosan-nanocomposites over MS	1413:1470	Adsorption of chitosan and chitosan-nanocomposites over MS is also evident by SEM and AFM.
31401281	2	60	theme	aggressive	227:236	arg1	media					238:242	aggressive media	227:242	aggressive media	227:242	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	2	61	from	metals	217:222	arg1	media					238:242	aggressive media	227:242	aggressive media	227:242	Besides its good green value, it is rarely used to protect metals in aggressive media in direct form due to rapid degradation of its molecules in acid.
31401281	6	62	theme	analyzing	952:960	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	62	theme	analyzing	952:960	arg1	techniques					962:971	surface analyzing techniques	944:971	surface analyzing techniques	944:971	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	0	63	theme	steel	67:71	arg1	protection					48:57	corrosion protection	38:57	corrosion protection of mild steel in aggressive chloride media	38:100	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	1	64	theme	important	126:134	arg1	bio-polymers					144:155	the important natural bio-polymers	122:155	the important natural bio-polymers	122:155	Chitosan is one of the important natural bio-polymers.
31401281	6	65	theme	surface	944:950	arg1	curves					867:872	Tafel polarization curves	848:872	Tafel polarization curves (TPC)	848:878	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	6	65	theme	surface	944:950	arg1	techniques					962:971	surface analyzing techniques	944:971	surface analyzing techniques	944:971	Corrosion behavior of the composites have been examined by Tafel polarization curves (TPC), single sine electrochemical impedance spectroscopy (SSEIS) and surface analyzing techniques.
31401281	0	66	theme	mild	62:65	arg1	steel					67:71	mild steel	62:71	mild steel	62:71	Chitosan based new nanocomposites for corrosion protection of mild steel in aggressive chloride media.
31401281	5	67	theme	successful	749:758	arg1	formation					760:768	successful formation	749:768	successful formation of nanocomposites	749:786	The prepared composites are investigated by UV-visible spectroscopy, FTIR spectroscopy, HRSEM, HRTEM and EDAX techniques, which have shown successful formation of nanocomposites.
31401281	1	68	theme	natural	136:142	arg1	bio-polymers					144:155	the important natural bio-polymers	122:155	the important natural bio-polymers	122:155	Chitosan is one of the important natural bio-polymers.
31401281	9	69	theme	main	1340:1343	arg1	reason					1345:1350	main reason	1340:1350	main reason of inhibition	1340:1364	Isotherm study suggests that adsorption of chitosan/composites over MS is main reason of inhibition and can be best defined with Frumkin isotherm.
31401281	4	70	theme	steel	567:571	arg1	protection					548:557	corrosion protection	538:557	corrosion protection of mild steel (MS)	538:576	In present work, cobalt and tin sulfide nanoparticles are used to synthesize chitosan-cobalt and chitosan-SnS2 nanocomposites, and used for corrosion protection of mild steel (MS) in 1 M HCl at room temperature.
30914366	5	0	dep	Gram-negative	1004:1016	arg1	coli					1031:1034	Escherichia coli	1019:1034	Escherichia coli	1019:1034	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	1	contain	possesses	907:915	arg1	film					902:905	the film	898:905	the film	898:905	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	1	contain	possesses	907:915	arg2	combinations					926:937	enhanced combinations	917:937	enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	917:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	1	2	theme	based	184:188	arg1	films					200:204	PVA/starch/citric acid (PVA/St/CA) based composite films	149:204	PVA/starch/citric acid (PVA/St/CA) based composite films	149:204	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	5	3	theme	antibacterial	976:988	arg1	activity					990:997	antibacterial activity	976:997	antibacterial activity	976:997	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	4	theme	Listeria	1056:1063	arg1	bacteria					1080:1087	Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	1004:1087	bacteria	1080:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	4	theme	Listeria	1056:1063	arg1	monocytogenes					1065:1077	Listeria monocytogenes	1056:1077	Listeria monocytogenes	1056:1077	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	4	5	theme	degradation	807:817	arg1	characteristics					872:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	5	6	dep	vapor	948:952	arg1	rate					967:970	transmission rate	954:970	transmission rate	954:970	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	7	theme	Gram-positive	1041:1053	arg1	bacteria					1080:1087	Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	1004:1087	bacteria	1080:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	7	theme	Gram-positive	1041:1053	arg1	monocytogenes					1065:1077	Listeria monocytogenes	1056:1077	Listeria monocytogenes	1056:1077	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	4	8	theme	swelling	770:777	arg1	characteristics					872:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	6	9	theme	fabricated	1185:1194	arg1	films					1206:1210	fabricated PVA-St-CA films	1185:1210	fabricated PVA-St-CA films	1185:1210	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	4	10	contain	possess	752:758	arg1	composition					722:732	The optimized film (composition)	702:733	The optimized film (composition)	702:733	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	4	10	contain	possess	752:758	arg2	characteristics					872:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics	760:886	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	6	11	theme	low	1136:1138	arg1	50 °C					1153:1157	50 °C	1153:1157	50 °C	1153:1157	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	11	theme	low	1136:1138	arg1	temperature					1140:1150	the low temperature	1132:1150	the low temperature (50 °C)	1132:1158	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	11	theme	low	1136:1138	arg1	duration					1169:1176	long duration	1164:1176	long duration (12 h)	1164:1183	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	11	theme	low	1136:1138	arg1	candidate					1249:1257	a potential candidate	1237:1257	a potential candidate for wound dressing applications	1237:1289	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	4	12	theme	in-vitro	798:805	arg1	degradation					807:817	in-vitro degradation	798:817	in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days)	798:870	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	1	13	theme	composite	190:198	arg1	films					200:204	PVA/starch/citric acid (PVA/St/CA) based composite films	149:204	PVA/starch/citric acid (PVA/St/CA) based composite films	149:204	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	5	14	theme	water	942:946	arg1	vapor					948:952	water vapor transmission rate and antibacterial activity	942:997	vapor	948:952	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	4	15	theme	%	841:841	arg1	degradation					843:853	45.5 ± 1.8-59.8 ± 0.4% degradation	820:853	45.5 ± 1.8-59.8 ± 0.4% degradation	820:853	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	3	16	theme	swelling	553:560	arg1	index					562:566	swelling index	553:566	swelling index	553:566	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	1	17	theme	films	200:204	arg1	competence					135:144	the competence	131:144	the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications	131:236	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	6	18	theme	PVA-St-CA	1196:1204	arg1	films					1206:1210	fabricated PVA-St-CA films	1185:1210	fabricated PVA-St-CA films	1185:1210	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	5	19	with	vapor	948:952	arg1	Gram-negative					1004:1016	Gram-negative	1004:1016	Gram-negative	1004:1016	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	19	with	vapor	948:952	arg1	bacteria					1080:1087	Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	1004:1087	bacteria	1080:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	19	with	vapor	948:952	arg1	monocytogenes					1065:1077	Listeria monocytogenes	1056:1077	Listeria monocytogenes	1056:1077	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	2	20	theme	temperature	347:357	arg1	optimality					320:329	the optimality	316:329	the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g)	316:414	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	4	21	theme	optimized	706:714	arg1	composition					722:732	The optimized film (composition)	702:733	The optimized film (composition)	702:733	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	2	22	theme	solution	446:453	arg1	casting					455:461	solution casting	446:461	solution casting based composite film fabrication	446:494	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	23	theme	crosslinking	334:345	arg1	60 °C					366:370	60 °C	366:370	60 °C	366:370	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	23	theme	crosslinking	334:345	arg1	temperature					347:357	crosslinking temperature	334:357	crosslinking temperature (40 to 60 °C)	334:371	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	24	theme	concentration	389:401	arg1	optimality					320:329	the optimality	316:329	the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g)	316:414	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	0	25	theme	alcohol/starch/glycerol/citric	26:55	arg1	films					72:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	4	26	theme	excellent	760:768	arg1	swelling					770:777	excellent swelling	760:777	excellent swelling (260.5 ± 2.9%)	760:792	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	4	26	theme	excellent	760:768	arg1	%					791:791	260.5 ± 2.9%	780:791	260.5 ± 2.9%	780:791	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	5	27	with	activity	990:997	arg1	Gram-negative					1004:1016	Gram-negative	1004:1016	Gram-negative	1004:1016	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	27	with	activity	990:997	arg1	bacteria					1080:1087	Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	1004:1087	bacteria	1080:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	5	27	with	activity	990:997	arg1	monocytogenes					1065:1077	Listeria monocytogenes	1056:1077	Listeria monocytogenes	1056:1077	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	2	28	dep	60 °C	366:370	arg1	to					363:364	to	363:364	to	363:364	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	6	29	theme	dressing	1269:1276	arg1	applications					1278:1289	wound dressing applications	1263:1289	wound dressing applications	1263:1289	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	1	30	theme	dressing	216:223	arg1	applications					225:236	wound dressing applications	210:236	wound dressing applications	210:236	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	1	31	dep	based	184:188	arg1	PVA/St/CA					173:181	PVA/St/CA	173:181	PVA/St/CA	173:181	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	1	31	dep	based	184:188	arg1	acid					167:170	PVA/starch/citric acid	149:170	PVA/starch/citric acid (PVA/St/CA)	149:182	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	5	32	theme	activity	990:997	arg1	combinations					926:937	enhanced combinations	917:937	enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	917:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	3	33	theme	based	580:584	arg1	biodegradability					586:601	solubility based biodegradability	569:601	solubility based biodegradability	569:601	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	5	34	theme	enhanced	917:924	arg1	combinations					926:937	enhanced combinations	917:937	enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	917:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	0	35	theme	composite	62:70	arg1	films					72:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	5	36	theme	transmission	954:965	arg1	rate					967:970	transmission rate	954:970	transmission rate	954:970	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	3	37	theme	antimicrobial	673:685	arg1	effectiveness					687:699	antimicrobial effectiveness	673:699	antimicrobial effectiveness	673:699	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	2	38	theme	PVA	274:276	arg1	composition					259:269	Literature reported composition	239:269	Literature reported composition of PVA, starch and glycerol	239:297	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	1	39	theme	wound	210:214	arg1	applications					225:236	wound dressing applications	210:236	wound dressing applications	210:236	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	0	40	theme	acid	57:60	arg1	films					72:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	2	41	theme	film	479:482	arg1	fabrication					484:494	composite film fabrication	469:494	composite film fabrication	469:494	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	3	42	theme	transmission	651:662	arg1	rate					664:667	water vapor transmission rate	639:667	water vapor transmission rate	639:667	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	2	43	dep	3 g	411:413	arg1	to					408:409	to	408:409	to	408:409	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	44	theme	composite	469:477	arg1	fabrication					484:494	composite film fabrication	469:494	composite film fabrication	469:494	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	3	45	theme	gel	604:606	arg1	fraction					608:615	gel fraction	604:615	gel fraction	604:615	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	6	46	theme	potential	1239:1247	arg1	temperature					1140:1150	the low temperature	1132:1150	the low temperature (50 °C)	1132:1158	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	46	theme	potential	1239:1247	arg1	duration					1169:1176	long duration	1164:1176	long duration (12 h)	1164:1183	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	46	theme	potential	1239:1247	arg1	candidate					1249:1257	a potential candidate	1237:1257	a potential candidate for wound dressing applications	1237:1289	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	3	47	theme	prepared	501:508	arg1	films					518:522	The prepared polymer films	497:522	The prepared polymer films	497:522	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	0	48	theme	films	72:76	arg1	Feasibility					0:10	Feasibility	0:10	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.	0:109	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	6	49	theme	wound	1263:1267	arg1	applications					1278:1289	wound dressing applications	1263:1289	wound dressing applications	1263:1289	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	2	50	theme	Literature	239:248	arg1	composition					259:269	Literature reported composition	239:269	Literature reported composition of PVA, starch and glycerol	239:297	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	51	theme	reported	250:257	arg1	composition					259:269	Literature reported composition	239:269	Literature reported composition of PVA, starch and glycerol	239:297	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	3	52	theme	polymer	510:516	arg1	films					518:522	The prepared polymer films	497:522	The prepared polymer films	497:522	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	4	53	theme	45.5 ± 1.8-59.8 ± 0.4	820:840	arg1	%					841:841	%	841:841	%	841:841	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	3	54	theme	water	639:643	arg1	rate					664:667	water vapor transmission rate	639:667	water vapor transmission rate	639:667	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	2	55	theme	glycerol	290:297	arg1	composition					259:269	Literature reported composition	239:269	Literature reported composition of PVA, starch and glycerol	239:297	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	3	56	theme	mechanical	618:627	arg1	strength					629:636	mechanical strength	618:636	mechanical strength	618:636	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	1	57	theme	PVA/starch/citric	149:165	arg1	PVA/St/CA					173:181	PVA/St/CA	173:181	PVA/St/CA	173:181	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	1	57	theme	PVA/starch/citric	149:165	arg1	acid					167:170	PVA/starch/citric acid	149:170	PVA/starch/citric acid (PVA/St/CA)	149:182	This work addresses the competence of PVA/starch/citric acid (PVA/St/CA) based composite films for wound dressing applications.
30914366	3	58	theme	solubility	569:578	arg1	biodegradability					586:601	solubility based biodegradability	569:601	solubility based biodegradability	569:601	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	5	59	theme	vapor	948:952	arg1	combinations					926:937	enhanced combinations	917:937	enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria	917:1087	Further, the film possesses enhanced combinations of water vapor transmission rate and antibacterial activity with Gram-negative (Escherichia coli) and Gram-positive (Listeria monocytogenes) bacteria.
30914366	3	60	theme	vapor	645:649	arg1	rate					664:667	water vapor transmission rate	639:667	water vapor transmission rate	639:667	The prepared polymer films have been characterized with swelling index, solubility based biodegradability, gel fraction, mechanical strength, water vapor transmission rate and antimicrobial effectiveness.
30914366	4	61	theme	film	716:719	arg1	composition					722:732	The optimized film (composition)	702:733	The optimized film (composition)	702:733	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
30914366	6	62	theme	long	1164:1167	arg1	12 h					1179:1182	12 h	1179:1182	12 h	1179:1182	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	62	theme	long	1164:1167	arg1	duration					1169:1176	long duration	1164:1176	long duration (12 h)	1164:1183	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	62	theme	long	1164:1167	arg1	temperature					1140:1150	the low temperature	1132:1150	the low temperature (50 °C)	1132:1158	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	6	62	theme	long	1164:1167	arg1	candidate					1249:1257	a potential candidate	1237:1257	a potential candidate for wound dressing applications	1237:1289	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	0	63	theme	dressing	88:95	arg1	applications					97:108	wound dressing applications	82:108	wound dressing applications	82:108	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	2	64	theme	starch	279:284	arg1	composition					259:269	Literature reported composition	239:269	Literature reported composition of PVA, starch and glycerol	239:297	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	6	65	theme	promising	1105:1113	arg1	characteristics					1115:1129	all these promising characteristics	1095:1129	all these promising characteristics	1095:1129	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	2	66	theme	acid	384:387	arg1	concentration					389:401	citric acid concentration	377:401	citric acid concentration (0.5 to 3 g)	377:414	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	2	66	theme	acid	384:387	arg1	3 g					411:413	3 g	411:413	3 g	411:413	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	6	67	dep	temperature	1140:1150	arg1	films					1206:1210	fabricated PVA-St-CA films	1185:1210	fabricated PVA-St-CA films	1185:1210	With all these promising characteristics, the low temperature (50 °C) and long duration (12 h) fabricated PVA-St-CA films have been inferred to be a potential candidate for wound dressing applications.
30914366	0	68	theme	wound	82:86	arg1	applications					97:108	wound dressing applications	82:108	wound dressing applications	82:108	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	0	69	theme	poly-vinyl	15:24	arg1	films					72:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	poly-vinyl alcohol/starch/glycerol/citric acid composite films	15:76	Feasibility of poly-vinyl alcohol/starch/glycerol/citric acid composite films for wound dressing applications.
30914366	2	70	theme	citric	377:382	arg1	acid					384:387	citric acid	377:387	citric acid concentration (0.5 to 3 g)	377:414	Literature reported composition of PVA, starch and glycerol were adopted and the optimality of crosslinking temperature (40 to 60 °C) and citric acid concentration (0.5 to 3 g) have been investigated during solution casting based composite film fabrication.
30914366	4	71	dep	degradation	807:817	arg1	degradation					843:853	45.5 ± 1.8-59.8 ± 0.4% degradation	820:853	45.5 ± 1.8-59.8 ± 0.4% degradation	820:853	The optimized film (composition) were analyzed to possess excellent swelling (260.5 ± 2.9%) and in-vitro degradation (45.5 ± 1.8-59.8 ± 0.4% degradation, during 14 days) characteristics.
29355639	5	0	theme	scanning	759:766	arg1	SEM					789:791	SEM	789:791	SEM	789:791	The micro-morphology of hydrogels were analyzed by scanning electron microscopy (SEM).
29355639	5	0	theme	scanning	759:766	arg1	microscopy					777:786	scanning electron microscopy	759:786	scanning electron microscopy (SEM)	759:792	The micro-morphology of hydrogels were analyzed by scanning electron microscopy (SEM).
29355639	4	1	theme	infrared	677:684	arg1	spectroscopy					686:697	infrared spectroscopy	677:697	infrared spectroscopy (FT-IR)	677:705	The chemical structures of this composite hydrogel were characterized by transform infrared spectroscopy (FT-IR).
29355639	4	1	theme	infrared	677:684	arg1	FT-IR					700:704	FT-IR	700:704	FT-IR	700:704	The chemical structures of this composite hydrogel were characterized by transform infrared spectroscopy (FT-IR).
29355639	7	2	theme	gelation	1118:1125	arg1	time					1127:1130	short gelation time	1112:1130	short gelation time	1112:1130	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	5	3	theme	electron	768:775	arg1	SEM					789:791	SEM	789:791	SEM	789:791	The micro-morphology of hydrogels were analyzed by scanning electron microscopy (SEM).
29355639	5	3	theme	electron	768:775	arg1	microscopy					777:786	scanning electron microscopy	759:786	scanning electron microscopy (SEM)	759:792	The micro-morphology of hydrogels were analyzed by scanning electron microscopy (SEM).
29355639	3	4	theme	oxidized	503:510	arg1	OKGM					532:535	OKGM	532:535	OKGM	532:535	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	4	theme	oxidized	503:510	arg1	glucomannan					519:529	oxidized konjac glucomannan	503:529	oxidized konjac glucomannan (OKGM)	503:536	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	5	theme	simple	358:363	arg1	method					378:383	a simple and nontoxic method	356:383	a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS)	356:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	6	6	theme	evaporation	893:903	arg1	rate					905:908	water evaporation rate	887:908	water evaporation rate	887:908	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	2	7	theme	biomedicine	305:315	arg1	field					296:300	the field	292:300	the field of biomedicine	292:315	It has been extensively researched and the application in the field of biomedicine is common.
29355639	6	8	theme	water	887:891	arg1	rate					905:908	water evaporation rate	887:908	water evaporation rate	887:908	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	7	9	theme	short	1112:1116	arg1	time					1127:1130	short gelation time	1112:1130	short gelation time	1112:1130	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	7	10	theme	swelling	1138:1145	arg1	ability					1147:1153	good swelling ability	1133:1153	good swelling ability	1133:1153	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	6	11	theme	different	986:994	arg1	means					996:1000	different means	986:1000	different means	986:1000	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	5	12	theme	hydrogels	732:740	arg1	micro-morphology					712:727	The micro-morphology	708:727	The micro-morphology of hydrogels	708:740	The micro-morphology of hydrogels were analyzed by scanning electron microscopy (SEM).
29355639	7	13	theme	properties	1093:1102	arg1	series					1083:1088	a series	1081:1088	a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility	1081:1252	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	3	14	theme	composite	413:421	arg1	hydrogel					423:430	composite hydrogel	413:430	composite hydrogel	413:430	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	7	15	theme	good	1133:1136	arg1	ability					1147:1153	good swelling ability	1133:1153	good swelling ability	1133:1153	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	1	16	with	biomaterial	147:157	arg1	performance					164:174	performance	164:174	performance	164:174	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	7	17	theme	excellent	1192:1200	arg1	hemocompatibility					1202:1218	excellent hemocompatibility	1192:1218	excellent hemocompatibility	1192:1218	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	1	18	theme	Hydrogel	106:113	arg1	dressing					121:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	18	theme	Hydrogel	106:113	arg1	better					184:189	better	184:189	better	184:189	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	18	theme	Hydrogel	106:113	arg1	type					139:142	a new type	133:142	a new type of biomaterial with performance that is better than traditional and biological dressings	133:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	4	19	theme	hydrogel	636:643	arg1	structures					607:616	The chemical structures	594:616	The chemical structures of this composite hydrogel	594:643	The chemical structures of this composite hydrogel were characterized by transform infrared spectroscopy (FT-IR).
29355639	7	20	theme	water	1168:1172	arg1	rate					1186:1189	appropriate water evaporation rate	1156:1189	appropriate water evaporation rate	1156:1189	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	6	21	theme	swelling	869:876	arg1	ability					878:884	swelling ability	869:884	swelling ability	869:884	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	7	22	theme	well	1224:1227	arg1	compatibility					1240:1252	well biological compatibility	1224:1252	well biological compatibility	1224:1252	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	1	23	theme	wound	115:119	arg1	dressing					121:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	23	theme	wound	115:119	arg1	better					184:189	better	184:189	better	184:189	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	23	theme	wound	115:119	arg1	type					139:142	a new type	133:142	a new type of biomaterial with performance that is better than traditional and biological dressings	133:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	24	theme	traditional	196:206	arg1	dressings					223:231	traditional and biological dressings	196:231	traditional and biological dressings	196:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	7	25	theme	evaporation	1174:1184	arg1	rate					1186:1189	appropriate water evaporation rate	1156:1189	appropriate water evaporation rate	1156:1189	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	3	26	theme	carboxymethyl	555:567	arg1	CMSS					587:590	CMSS	587:590	CMSS	587:590	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	26	theme	carboxymethyl	555:567	arg1	sulfate					578:584	carboxymethyl chitosan sulfate	555:584	carboxymethyl chitosan sulfate (CMSS)	555:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	27	theme	hydrogel	423:430	arg1	type					405:408	a new type	399:408	a new type	399:408	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	28	theme	nontoxic	369:376	arg1	method					378:383	a simple and nontoxic method	356:383	a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS)	356:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	8	29	theme	wound	1338:1342	arg1	hydrogels					1311:1319	this composite hydrogels	1296:1319	this composite hydrogels	1296:1319	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	8	29	theme	wound	1338:1342	arg1	dressing					1344:1351	a wound dressing	1336:1351	a wound dressing	1336:1351	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	1	30	theme	biological	212:221	arg1	dressings					223:231	traditional and biological dressings	196:231	traditional and biological dressings	196:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	7	31	theme	appropriate	1156:1166	arg1	rate					1186:1189	appropriate water evaporation rate	1156:1189	appropriate water evaporation rate	1156:1189	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	7	32	contain	have	1076:1079	arg1	hydrogels					1066:1074	this composite hydrogels	1051:1074	this composite hydrogels	1051:1074	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	7	32	contain	have	1076:1079	arg2	series					1083:1088	a series	1081:1088	a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility	1081:1252	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	3	33	theme	Schiff-base	458:468	arg1	reaction					470:477	the Schiff-base reaction	454:477	the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS)	454:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	0	34	theme	chitosan	50:57	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of carboxymethyl chitosan sulfate/oxidized konjac glucomannan hydrogels.
29355639	0	34	theme	chitosan	50:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of carboxymethyl chitosan sulfate/oxidized konjac glucomannan hydrogels.
29355639	7	35	theme	composite	1056:1064	arg1	hydrogels					1066:1074	this composite hydrogels	1051:1074	this composite hydrogels	1051:1074	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	8	36	theme	composite	1301:1309	arg1	hydrogels					1311:1319	this composite hydrogels	1296:1319	this composite hydrogels	1296:1319	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	8	36	theme	composite	1301:1309	arg1	dressing					1344:1351	a wound dressing	1336:1351	a wound dressing	1336:1351	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	2	37	from	application	277:287	arg1	field					296:300	the field	292:300	the field of biomedicine	292:315	It has been extensively researched and the application in the field of biomedicine is common.
29355639	4	38	theme	composite	626:634	arg1	hydrogel					636:643	this composite hydrogel	621:643	this composite hydrogel	621:643	The chemical structures of this composite hydrogel were characterized by transform infrared spectroscopy (FT-IR).
29355639	0	39	theme	carboxymethyl	36:48	arg1	chitosan					50:57	carboxymethyl chitosan	36:57	carboxymethyl chitosan	36:57	Preparation and characterization of carboxymethyl chitosan sulfate/oxidized konjac glucomannan hydrogels.
29355639	6	40	theme	gelation	854:861	arg1	time					863:866	gelation time	854:866	gelation time	854:866	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	8	41	used	used	1328:1331	arg2	dressing					1344:1351	a wound dressing	1336:1351	a wound dressing	1336:1351	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	8	41	used	used	1328:1331	arg2	hydrogels					1311:1319	this composite hydrogels	1296:1319	this composite hydrogels	1296:1319	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	0	42	theme	konjac	76:81	arg1	hydrogels					95:103	konjac glucomannan hydrogels	76:103	konjac glucomannan hydrogels	76:103	Preparation and characterization of carboxymethyl chitosan sulfate/oxidized konjac glucomannan hydrogels.
29355639	6	43	theme	biological	935:944	arg1	compatibility					946:958	biological compatibility	935:958	biological compatibility	935:958	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	3	44	theme	chitosan	569:576	arg1	CMSS					587:590	CMSS	587:590	CMSS	587:590	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	44	theme	chitosan	569:576	arg1	sulfate					578:584	carboxymethyl chitosan sulfate	555:584	carboxymethyl chitosan sulfate (CMSS)	555:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	1	45	theme	new	135:137	arg1	dressing					121:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	45	theme	new	135:137	arg1	better					184:189	better	184:189	better	184:189	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	45	theme	new	135:137	arg1	type					139:142	a new type	133:142	a new type of biomaterial with performance that is better than traditional and biological dressings	133:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	3	46	theme	new	401:403	arg1	type					405:408	a new type	399:408	a new type	399:408	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	6	47	theme	hydrogels	834:842	arg1	Meanwhile					795:803	Meanwhile	795:803	Meanwhile	795:803	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	47	theme	hydrogels	834:842	arg1	properties					810:819	the properties	806:819	the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility	806:958	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	3	48	theme	sulfate	578:584	arg1	amino					546:550	the amino	542:550	the amino of carboxymethyl chitosan sulfate (CMSS)	542:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	48	theme	sulfate	578:584	arg1	aldehyde					491:498	the aldehyde	487:498	the aldehyde of oxidized konjac glucomannan (OKGM)	487:536	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	6	49	theme	composite	824:832	arg1	rate					905:908	water evaporation rate	887:908	water evaporation rate	887:908	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	49	theme	composite	824:832	arg1	hydrogels					834:842	composite hydrogels	824:842	composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility	824:958	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	49	theme	composite	824:832	arg1	ability					878:884	swelling ability	869:884	swelling ability	869:884	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	49	theme	composite	824:832	arg1	potential					921:929	hemolytic potential	911:929	hemolytic potential	911:929	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	49	theme	composite	824:832	arg1	compatibility					946:958	biological compatibility	935:958	biological compatibility	935:958	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	6	49	theme	composite	824:832	arg1	time					863:866	gelation time	854:866	gelation time	854:866	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	8	50	theme	excellent	1273:1281	arg1	performance					1283:1293	these excellent performance	1267:1293	these excellent performance	1267:1293	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	8	51	theme	injured	1362:1368	arg1	skin					1370:1373	injured skin	1362:1373	injured skin	1362:1373	Considering these excellent performance, this composite hydrogels can be used as a wound dressing to treat injured skin.
29355639	1	52	theme	biomaterial	147:157	arg1	dressing					121:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing	106:128	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	52	theme	biomaterial	147:157	arg1	better					184:189	better	184:189	better	184:189	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	1	52	theme	biomaterial	147:157	arg1	type					139:142	a new type	133:142	a new type of biomaterial with performance that is better than traditional and biological dressings	133:231	Hydrogel wound dressing is a new type of biomaterial with performance that is better than traditional and biological dressings.
29355639	7	53	theme	biological	1229:1238	arg1	compatibility					1240:1252	well biological compatibility	1224:1252	well biological compatibility	1224:1252	The results gained from these studies show that this composite hydrogels have a series of properties such as short gelation time, good swelling ability, appropriate water evaporation rate, excellent hemocompatibility and well biological compatibility.
29355639	3	54	theme	konjac	512:517	arg1	OKGM					532:535	OKGM	532:535	OKGM	532:535	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	54	theme	konjac	512:517	arg1	glucomannan					519:529	oxidized konjac glucomannan	503:529	oxidized konjac glucomannan (OKGM)	503:536	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	4	55	theme	chemical	598:605	arg1	structures					607:616	The chemical structures	594:616	The chemical structures of this composite hydrogel	594:643	The chemical structures of this composite hydrogel were characterized by transform infrared spectroscopy (FT-IR).
29355639	0	56	theme	glucomannan	83:93	arg1	hydrogels					95:103	konjac glucomannan hydrogels	76:103	konjac glucomannan hydrogels	76:103	Preparation and characterization of carboxymethyl chitosan sulfate/oxidized konjac glucomannan hydrogels.
29355639	6	57	theme	hemolytic	911:919	arg1	potential					921:929	hemolytic potential	911:929	hemolytic potential	911:929	Meanwhile, the properties of composite hydrogels including gelation time, swelling ability, water evaporation rate, hemolytic potential and biological compatibility were also investigated in different means.
29355639	3	58	theme	glucomannan	519:529	arg1	amino					546:550	the amino	542:550	the amino of carboxymethyl chitosan sulfate (CMSS)	542:591	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
29355639	3	58	theme	glucomannan	519:529	arg1	aldehyde					491:498	the aldehyde	487:498	the aldehyde of oxidized konjac glucomannan (OKGM)	487:536	In this study, we developed a simple and nontoxic method for preparing a new type of composite hydrogel, which formed through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan sulfate (CMSS).
31417166	7	0	theme	activity	1292:1299	arg1	Analysis					1266:1273	Analysis	1266:1273	Analysis of glycosylation activity	1266:1299	Analysis of glycosylation activity exposed two strong glycoproteins as 300-kDa and 240-kDa.
31417166	10	1	theme	cell	1875:1878	arg1	walls					1880:1884	the living skeletal cell walls	1855:1884	the living skeletal cell walls	1855:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	6	2	theme	molecular	1231:1239	arg1	mass					1241:1244	a high molecular mass	1224:1244	a high molecular mass protein	1224:1252	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	1	3	theme	crystal	237:243	arg1	deposition					245:254	crystal deposition	237:254	crystal deposition	237:254	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	10	4	theme	unidentified	1766:1777	arg1	process					1779:1785	an as yet unidentified process	1756:1785	an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls	1756:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	6	5	theme	OM	1212:1213	arg1	analysis					1196:1203	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis	1123:1203	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM	1123:1213	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	3	6	theme	calcium	758:764	arg1	crystals					776:783	calcium carbonate crystals	758:783	calcium carbonate crystals	758:783	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	8	7	from	present	1477:1483	arg1	skeleton					1492:1499	the skeleton	1488:1499	the skeleton	1488:1499	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	0	8	theme	coralline	108:116	arg1	foecundum					135:143	the crustose coralline alga Leptophytum foecundum	95:143	the crustose coralline alga Leptophytum foecundum	95:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	5	9	theme	Leptophytum	1100:1110	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	10	10	theme	living	1794:1799	arg1	coralline					1801:1809	the living coralline	1790:1809	the living coralline	1790:1809	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	5	11	from	presence	1050:1057	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	1	12	theme	marine	183:188	arg1	calcifiers					190:199	marine calcifiers	183:199	marine calcifiers	183:199	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	4	13	theme	control	966:972	arg1	mineralization					903:916	the mineralization	899:916	the mineralization of calcium carbonate crystals and environmental control	899:972	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	0	14	theme	Leptophytum	123:133	arg1	foecundum					135:143	the crustose coralline alga Leptophytum foecundum	95:143	the crustose coralline alga Leptophytum foecundum	95:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	3	15	theme	crystals	776:783	arg1	mineralization					740:753	mineralization	740:753	mineralization	740:753	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	3	15	theme	crystals	776:783	arg1	formation					726:734	structural formation	715:734	structural formation	715:734	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	3	16	theme	organic	581:587	arg1	matrices					589:596	organic matrices	581:596	organic matrices	581:596	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	9	17	theme	chitin-rich	1539:1549	arg1	OM					1560:1561	the chitin-rich skeletal OM	1535:1561	the chitin-rich skeletal OM of coralline algae	1535:1580	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	10	18	from	process	1779:1785	arg1	coralline					1801:1809	the living coralline	1790:1809	the living coralline	1790:1809	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	4	19	theme	carbonate	929:937	arg1	crystals					939:946	calcium carbonate crystals	921:946	calcium carbonate crystals	921:946	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	5	20	theme	coralline	1085:1093	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	10	21	theme	living	1859:1864	arg1	walls					1880:1884	the living skeletal cell walls	1855:1884	the living skeletal cell walls	1855:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	8	22	dep	%	1460:1460	arg1	30					1458:1459	30	1458:1459	30	1458:1459	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	5	23	attach	presence	1050:1057	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	5	23	attach	presence	1050:1057	arg2	chitin					1062:1067	chitin	1062:1067	chitin	1062:1067	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	2	24	theme	crustose	493:500	arg1	algae					512:516	crustose coralline algae	493:516	crustose coralline algae	493:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	3	25	from	characterization	561:576	arg1	process					623:629	the biomineralization process	601:629	the biomineralization process of this species	601:645	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	7	26	theme	glycosylation	1278:1290	arg1	activity					1292:1299	glycosylation activity	1278:1299	glycosylation activity	1278:1299	Analysis of glycosylation activity exposed two strong glycoproteins as 300-kDa and 240-kDa.
31417166	3	27	theme	biomineralization	605:621	arg1	process					623:629	the biomineralization process	601:629	the biomineralization process of this species	601:645	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	9	28	theme	coralline	1566:1574	arg1	algae					1576:1580	coralline algae	1566:1580	coralline algae	1566:1580	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	2	29	theme	crystallization	474:488	arg1	aspect					464:469	the biological aspect	449:469	the biological aspect of crystallization in crustose coralline algae	449:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	8	30	theme	collected	1395:1403	arg1	specimens					1405:1413	live collected specimens	1390:1413	live collected specimens	1390:1413	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	0	31	theme	carbonate	82:90	arg1	crystallization					55:69	the crystallization	51:69	the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum	51:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	9	32	theme	key	1590:1592	arg1	role					1594:1597	a key role	1588:1597	a key role	1588:1597	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	10	33	from	formation	1829:1837	arg1	walls					1880:1884	the living skeletal cell walls	1855:1884	the living skeletal cell walls	1855:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	1	34	theme	biological/environmental	334:357	arg1	control					359:365	biological/environmental control	334:365	biological/environmental control	334:365	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	2	35	theme	matrix	439:444	arg1	properties					392:401	the functional properties	377:401	the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	377:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	1	36	theme	deposition	245:254	arg1	regulation					223:232	the regulation	219:232	the regulation of crystal deposition	219:254	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	9	37	theme	biocalcification	1606:1621	arg1	process					1623:1629	the biocalcification process	1602:1629	the biocalcification process	1602:1629	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	2	38	theme	skeletal	422:429	arg1	matrix					439:444	the chitin-rich skeletal organic matrix	406:444	the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	406:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	0	39	theme	skeletal	24:31	arg1	matrix					41:46	chitin-rich skeletal organic matrix	12:46	chitin-rich skeletal organic matrix	12:46	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	1	40	theme	crystalline	265:275	arg1	structure					277:285	crystalline structure	265:285	crystalline structure	265:285	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	8	41	from	skeleton	1492:1499	arg1	present					1477:1483	present	1477:1483	present	1477:1483	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	8	42	attach	present	1477:1483	arg1	skeleton					1492:1499	the skeleton	1488:1499	the skeleton	1488:1499	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	8	42	attach	present	1477:1483	arg2	aragonite					1462:1470	up to 30% aragonite	1452:1470	up to 30% aragonite	1452:1470	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	4	43	theme	organic	807:813	arg1	proteins					822:829	skeletal organic matrix proteins	798:829	skeletal organic matrix proteins	798:829	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	10	44	theme	aragonite	1726:1734	arg1	formation					1713:1721	the formation	1709:1721	the formation of aragonite	1709:1734	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	7	45	gly	glycoproteins	1320:1332	arg1	glycoproteins					1320:1332	two strong glycoproteins	1309:1332	two strong glycoproteins	1309:1332	Analysis of glycosylation activity exposed two strong glycoproteins as 300-kDa and 240-kDa.
31417166	5	46	theme	OM	1034:1035	arg1	investigation					1004:1016	the analytical investigation	989:1016	the analytical investigation of the skeletal OM	989:1035	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	2	47	from	properties	392:401	arg1	aspect					464:469	the biological aspect	449:469	the biological aspect of crystallization in crustose coralline algae	449:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	6	48	theme	electrophoresis	1169:1183	arg1	analysis					1196:1203	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis	1123:1203	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM	1123:1213	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	7	49	theme	strong	1313:1318	arg1	glycoproteins					1320:1332	two strong glycoproteins	1309:1332	two strong glycoproteins	1309:1332	Analysis of glycosylation activity exposed two strong glycoproteins as 300-kDa and 240-kDa.
31417166	3	50	theme	components	700:709	arg1	functions					677:685	the functions	673:685	the functions of these key components for structural formation and mineralization of calcium carbonate crystals	673:783	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	9	51	theme	skeletal	1551:1558	arg1	OM					1560:1561	the chitin-rich skeletal OM	1535:1561	the chitin-rich skeletal OM of coralline algae	1535:1580	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	3	52	theme	species	639:645	arg1	process					623:629	the biomineralization process	601:629	the biomineralization process of this species	601:645	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	6	53	theme	high	1226:1229	arg1	mass					1241:1244	a high molecular mass	1224:1244	a high molecular mass protein	1224:1252	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	0	54	theme	matrix	41:46	arg1	role					4:7	The role	0:7	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.	0:144	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	3	55	theme	structural	715:724	arg1	formation					726:734	structural formation	715:734	structural formation	715:734	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	2	56	from	matrix	439:444	arg1	aspect					464:469	the biological aspect	449:469	the biological aspect of crystallization in crustose coralline algae	449:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	3	57	theme	carbonate	766:774	arg1	crystals					776:783	calcium carbonate crystals	758:783	calcium carbonate crystals	758:783	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	0	58	theme	alga	118:121	arg1	foecundum					135:143	the crustose coralline alga Leptophytum foecundum	95:143	the crustose coralline alga Leptophytum foecundum	95:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	9	59	theme	Mg-calcite	1660:1669	arg1	formation					1647:1655	the formation	1643:1655	the formation of Mg-calcite	1643:1669	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	4	60	theme	crystals	939:946	arg1	mineralization					903:916	the mineralization	899:916	the mineralization of calcium carbonate crystals and environmental control	899:972	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	3	61	theme	matrices	589:596	arg1	characterization					561:576	the characterization	557:576	the characterization of organic matrices in the biomineralization process of this species	557:645	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	9	62	theme	algae	1576:1580	arg1	OM					1560:1561	the chitin-rich skeletal OM	1535:1561	the chitin-rich skeletal OM of coralline algae	1535:1580	Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite.
31417166	4	63	theme	environmental	952:964	arg1	control					966:972	environmental control	952:972	environmental control	952:972	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	0	64	from	crystallization	55:69	arg1	foecundum					135:143	the crustose coralline alga Leptophytum foecundum	95:143	the crustose coralline alga Leptophytum foecundum	95:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	8	65	located	present	1477:1483	arg1	skeleton					1492:1499	the skeleton	1488:1499	the skeleton	1488:1499	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	8	65	located	present	1477:1483	arg2	aragonite					1462:1470	up to 30% aragonite	1452:1470	up to 30% aragonite	1452:1470	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	5	66	theme	crustose	1076:1083	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	10	67	theme	skeletal	1866:1873	arg1	walls					1880:1884	the living skeletal cell walls	1855:1884	the living skeletal cell walls	1855:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	8	68	dep	aragonite	1462:1470	arg1	%					1460:1460	%	1460:1460	%	1460:1460	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	4	69	theme	calcium	921:927	arg1	crystals					939:946	calcium carbonate crystals	921:946	calcium carbonate crystals	921:946	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	5	70	theme	alga	1095:1098	arg1	foecundum					1112:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	the crustose coralline alga Leptophytum foecundum	1072:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	8	71	dep	30	1458:1459	arg1	to					1455:1456	to	1455:1456	to	1455:1456	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	1	72	dep	such	257:260	arg1	as					262:263	as	262:263	as	262:263	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	6	73	theme	mass	1241:1244	arg1	protein					1246:1252	a high molecular mass protein	1224:1252	a high molecular mass protein	1224:1252	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	0	74	theme	calcium	74:80	arg1	carbonate					82:90	calcium carbonate	74:90	calcium carbonate	74:90	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	1	75	theme	mineralization	302:315	arg1	initiation					288:297	initiation	288:297	initiation of mineralization	288:315	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	1	76	theme	organic	150:156	arg1	OM					166:167	OM	166:167	OM	166:167	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	1	76	theme	organic	150:156	arg1	matrix					158:163	The organic matrix	146:163	The organic matrix (OM) contained in marine calcifiers	146:199	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	0	77	from	role	4:7	arg1	crystallization					55:69	the crystallization	51:69	the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum	51:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	8	78	theme	biominerals	1375:1385	arg1	study					1362:1366	Our study	1358:1366	Our study of the biominerals of live collected specimens	1358:1413	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	5	79	theme	analytical	993:1002	arg1	investigation					1004:1016	the analytical investigation	989:1016	the analytical investigation of the skeletal OM	989:1035	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	8	80	theme	live	1390:1393	arg1	specimens					1405:1413	live collected specimens	1390:1413	live collected specimens	1390:1413	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	0	81	theme	crustose	99:106	arg1	foecundum					135:143	the crustose coralline alga Leptophytum foecundum	95:143	the crustose coralline alga Leptophytum foecundum	95:143	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	2	82	theme	coralline	502:510	arg1	algae					512:516	crustose coralline algae	493:516	crustose coralline algae	493:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	2	83	from	aspect	464:469	arg1	properties					392:401	the functional properties	377:401	the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	377:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	2	83	from	aspect	464:469	arg1	algae					512:516	crustose coralline algae	493:516	crustose coralline algae	493:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	8	84	theme	specimens	1405:1413	arg1	biominerals					1375:1385	the biominerals	1371:1385	the biominerals of live collected specimens	1371:1413	Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton.
31417166	2	85	theme	organic	431:437	arg1	matrix					439:444	the chitin-rich skeletal organic matrix	406:444	the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	406:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	0	86	theme	chitin-rich	12:22	arg1	matrix					41:46	chitin-rich skeletal organic matrix	12:46	chitin-rich skeletal organic matrix	12:46	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	10	87	theme	aragonite	1842:1850	arg1	formation					1829:1837	the formation	1825:1837	the formation of aragonite in the living skeletal cell walls	1825:1884	In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
31417166	2	88	theme	chitin-rich	410:420	arg1	matrix					439:444	the chitin-rich skeletal organic matrix	406:444	the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	406:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	0	89	theme	organic	33:39	arg1	matrix					41:46	chitin-rich skeletal organic matrix	12:46	chitin-rich skeletal organic matrix	12:46	The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
31417166	2	90	theme	biological	453:462	arg1	aspect					464:469	the biological aspect	449:469	the biological aspect of crystallization in crustose coralline algae	449:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	2	91	theme	functional	381:390	arg1	properties					392:401	the functional properties	377:401	the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae	377:516	However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated.
31417166	5	92	theme	chitin	1062:1067	arg1	presence					1050:1057	the presence	1046:1057	the presence of chitin in the crustose coralline alga Leptophytum foecundum	1046:1120	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	1	93	contain	has	201:203	arg2	role					211:214	a key role	205:214	a key role	205:214	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	1	93	contain	has	201:203	arg1	OM					166:167	OM	166:167	OM	166:167	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	1	93	contain	has	201:203	arg1	matrix					158:163	The organic matrix	146:163	The organic matrix (OM) contained in marine calcifiers	146:199	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	5	94	theme	skeletal	1025:1032	arg1	OM					1034:1035	the skeletal OM	1021:1035	the skeletal OM	1021:1035	Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum.
31417166	4	95	theme	skeletal	798:805	arg1	proteins					822:829	skeletal organic matrix proteins	798:829	skeletal organic matrix proteins	798:829	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31417166	6	96	theme	sulfate-polyacrylamide	1142:1163	arg1	SDS-PAGE					1186:1193	SDS-PAGE	1186:1193	SDS-PAGE	1186:1193	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	6	96	theme	sulfate-polyacrylamide	1142:1163	arg1	electrophoresis					1169:1183	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis	1123:1183	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM	1123:1213	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	1	97	theme	key	207:209	arg1	role					211:214	a key role	205:214	a key role	205:214	The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control.
31417166	6	98	theme	gel	1165:1167	arg1	SDS-PAGE					1186:1193	SDS-PAGE	1186:1193	SDS-PAGE	1186:1193	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	6	98	theme	gel	1165:1167	arg1	electrophoresis					1169:1183	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis	1123:1183	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM	1123:1213	The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa.
31417166	3	99	theme	key	696:698	arg1	components					700:709	these key components	690:709	these key components	690:709	Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals.
31417166	4	100	theme	matrix	815:820	arg1	proteins					822:829	skeletal organic matrix proteins	798:829	skeletal organic matrix proteins	798:829	We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control.
31560145	0	0	theme	case	80:83	arg1	study					85:89	A case study	78:89	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.	0:102	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.
31560145	5	1	theme	potential	748:756	arg1	measurements					758:769	Zeta potential measurements	743:769	Zeta potential measurements	743:769	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	11	2	theme	flocculation	1868:1879	arg1	efficiency					1881:1890	The flocculation efficiency	1864:1890	The flocculation efficiency	1864:1890	The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
31560145	1	3	theme	aluminum	130:137	arg1	flocculants					150:160	aluminum (Al)-based flocculants	130:160	aluminum (Al)-based flocculants in the treatment of drinking water	130:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	3	4	from	effective	498:506	arg1	turbidity					520:528	reducing turbidity	511:528	reducing turbidity	511:528	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	8	5	theme	environment-friendly	1512:1531	arg1	flocculant					1543:1552	an environment-friendly composite flocculant	1509:1552	an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants	1509:1623	The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants.
31560145	8	6	theme	inorganic	1603:1611	arg1	flocculants					1613:1623	inorganic flocculants	1603:1623	inorganic flocculants	1603:1623	The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants.
31560145	5	7	theme	dispersion	787:796	arg1	analysis					798:805	photometric dispersion analysis	775:805	photometric dispersion analysis	775:805	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	8	8	theme	composite	1533:1541	arg1	flocculant					1543:1552	an environment-friendly composite flocculant	1509:1552	an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants	1509:1623	The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants.
31560145	10	9	from	turbidity	1818:1826	arg1	effective					1796:1804	effective	1796:1804	effective	1796:1804	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	10	9	from	turbidity	1818:1826	arg1	efficiency					1763:1772	The coagulation efficiency	1747:1772	The coagulation efficiency of CMP-AlCl3	1747:1785	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	7	10	theme	flocculating	1382:1393	arg1	-2.28 mV					1423:1430	-2.28 mV	1423:1430	-2.28 mV	1423:1430	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	10	theme	flocculating	1382:1393	arg1	%					1440:1440	98.0%	1436:1440	98.0%	1436:1440	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	10	theme	flocculating	1382:1393	arg1	activity					1395:1402	the maximum flocculating activity	1370:1402	the maximum flocculating activity	1370:1402	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	10	theme	flocculating	1382:1393	arg1	potential					1356:1364	the zeta potential	1347:1364	the zeta potential	1347:1364	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	5	11	theme	bridges	1001:1007	arg1	absorption					958:967	absorption	958:967	absorption	958:967	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	5	11	theme	bridges	1001:1007	arg1	development					977:987	the development	973:987	the development of particle bridges	973:1007	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	1	12	theme	human	225:229	arg1	reasons					238:244	human health reasons	225:244	human health reasons	225:244	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	2	13	theme	novel	264:268	arg1	flocculant					280:289	a novel composite flocculant	262:289	a novel composite flocculant	262:289	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	9	14	theme	PRACTITIONER	1626:1637	arg1	POINTS					1639:1644	PRACTITIONER POINTS	1626:1644	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.	1626:1745	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	9	15	theme	flocculant	1665:1674	arg1	CMP-AlCl3					1676:1684	A novel composite flocculant CMP-AlCl3	1647:1684	A novel composite flocculant CMP-AlCl3	1647:1684	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	1	16	from	use	123:125	arg1	treatment					169:177	the treatment	165:177	the treatment of drinking water	165:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	3	17	theme	reducing	511:518	arg1	turbidity					520:528	reducing turbidity	511:528	reducing turbidity	511:528	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	11	18	theme	potential	1932:1940	arg1	analysis					1942:1949	Zeta potential analysis	1927:1949	Zeta potential analysis	1927:1949	The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
31560145	1	19	theme	health	231:236	arg1	reasons					238:244	human health reasons	225:244	human health reasons	225:244	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	7	20	theme	model	1257:1261	arg1	suspension					1270:1279	the model kaolin suspension	1253:1279	the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units	1253:1344	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	10	21	theme	solo	1837:1840	arg1	use					1842:1844	the solo use	1833:1844	the solo use of CMP or AlCl3	1833:1860	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	9	22	dep	POINTS	1639:1644	arg1	achieved					1690:1697	achieved	1690:1697	was achieved by combining CMP and AlCl3 for water treatment	1686:1744	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	4	23	theme	treatment	596:604	arg1	conditions					606:615	The optimum treatment conditions	584:615	The optimum treatment conditions assessed by a response surface methodology	584:658	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	10	24	theme	CMP-AlCl3	1777:1785	arg1	effective					1796:1804	effective	1796:1804	effective	1796:1804	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	10	24	theme	CMP-AlCl3	1777:1785	arg1	efficiency					1763:1772	The coagulation efficiency	1747:1772	The coagulation efficiency of CMP-AlCl3	1747:1785	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	2	25	theme	lab-scale	348:356	arg1	system					368:373	a lab-scale, jar test system	346:373	a lab-scale, jar test system for the flocculation of kaolin	346:404	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	6	26	theme	composite	1020:1028	arg1	flocculant					1030:1039	the composite flocculant	1016:1039	the composite flocculant	1016:1039	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	10	27	theme	coagulation	1751:1761	arg1	effective					1796:1804	effective	1796:1804	effective	1796:1804	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	10	27	theme	coagulation	1751:1761	arg1	efficiency					1763:1772	The coagulation efficiency	1747:1772	The coagulation efficiency of CMP-AlCl3	1747:1785	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	10	28	theme	AlCl3	1856:1860	arg1	use					1842:1844	the solo use	1833:1844	the solo use of CMP or AlCl3	1833:1860	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	5	29	theme	charge	867:872	arg1	neutralization					874:887	charge neutralization	867:887	charge neutralization	867:887	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	11	30	theme	electron	1972:1979	arg1	microscopy					1981:1990	electron microscopy	1972:1990	electron microscopy	1972:1990	The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
31560145	1	31	dep	aluminum	130:137	arg1	Al					140:141	Al	140:141	Al	140:141	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	1	32	theme	-based	143:148	arg1	flocculants					150:160	aluminum (Al)-based flocculants	130:160	aluminum (Al)-based flocculants in the treatment of drinking water	130:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	11	33	theme	Zeta	1927:1930	arg1	analysis					1942:1949	Zeta potential analysis	1927:1949	Zeta potential analysis	1927:1949	The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
31560145	4	34	theme	13.03 mg/L	686:695	arg1	carboxymethylpullulan					697:717	13.03 mg/L carboxymethylpullulan	686:717	13.03 mg/L carboxymethylpullulan	686:717	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	3	35	theme	solo	539:542	arg1	use					544:546	the solo use	535:546	the solo use of carboxymethylpullulan or AlCl3	535:580	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	1	36	theme	flocculants	150:160	arg1	use					123:125	the use	119:125	the use of aluminum (Al)-based flocculants in the treatment of drinking water	119:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	3	37	theme	AlCl3	576:580	arg1	use					544:546	the solo use	535:546	the solo use of carboxymethylpullulan or AlCl3	535:580	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	0	38	with	study	85:89	arg1	kaolin					96:101	kaolin	96:101	kaolin	96:101	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.
31560145	5	39	theme	particle	992:999	arg1	bridges					1001:1007	particle bridges	992:1007	particle bridges	992:1007	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	7	40	theme	maximum	1374:1380	arg1	-2.28 mV					1423:1430	-2.28 mV	1423:1430	-2.28 mV	1423:1430	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	40	theme	maximum	1374:1380	arg1	%					1440:1440	98.0%	1436:1440	98.0%	1436:1440	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	40	theme	maximum	1374:1380	arg1	activity					1395:1402	the maximum flocculating activity	1370:1402	the maximum flocculating activity	1370:1402	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	40	theme	maximum	1374:1380	arg1	potential					1356:1364	the zeta potential	1347:1364	the zeta potential	1347:1364	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	0	41	theme	carboxymethylpullulan-AlCl3	14:40	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.	0:102	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.
31560145	5	42	contain	had	831:833	arg2	influence					854:862	a more significant influence	835:862	a more significant influence on charge neutralization	835:887	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	5	42	contain	had	831:833	arg1	AlCl3					825:829	AlCl3	825:829	AlCl3	825:829	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	4	43	theme	94.87 mg/L	724:733	arg1	AlCl3					735:739	94.87 mg/L AlCl3	724:739	94.87 mg/L AlCl3	724:739	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	9	44	theme	novel	1649:1653	arg1	CMP-AlCl3					1676:1684	A novel composite flocculant CMP-AlCl3	1647:1684	A novel composite flocculant CMP-AlCl3	1647:1684	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	2	45	theme	test	363:366	arg1	system					368:373	a lab-scale, jar test system	346:373	a lab-scale, jar test system for the flocculation of kaolin	346:404	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	7	46	theme	90 mg/L	1204:1210	arg1	carboxymethylpullulan-AlCl3					1212:1238	90 mg/L carboxymethylpullulan-AlCl3	1204:1238	90 mg/L carboxymethylpullulan-AlCl3	1204:1238	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	6	47	theme	environment	1176:1186	arg1	pollution					1188:1196	secondary environment pollution	1166:1196	secondary environment pollution	1166:1196	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	10	48	theme	reducing	1809:1816	arg1	turbidity					1818:1826	reducing turbidity	1809:1826	reducing turbidity	1809:1826	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	7	49	theme	turbidity	1330:1338	arg1	units					1340:1344	50 nephelometric turbidity units	1313:1344	50 nephelometric turbidity units	1313:1344	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	3	50	theme	carboxymethylpullulan	551:571	arg1	use					544:546	the solo use	535:546	the solo use of carboxymethylpullulan or AlCl3	535:580	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	4	51	theme	surface	640:646	arg1	methodology					648:658	a response surface methodology	629:658	a response surface methodology	629:658	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	5	52	theme	significant	842:852	arg1	influence					854:862	a more significant influence	835:862	a more significant influence on charge neutralization	835:887	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	4	53	theme	response	631:638	arg1	methodology					648:658	a response surface methodology	629:658	a response surface methodology	629:658	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	11	54	theme	surface	1952:1958	arg1	morphology					1960:1969	surface morphology	1952:1969	surface morphology	1952:1969	The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
31560145	5	55	theme	photometric	775:785	arg1	analysis					798:805	photometric dispersion analysis	775:805	photometric dispersion analysis	775:805	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	1	56	from	treatment	169:177	arg1	use					123:125	the use	119:125	the use of aluminum (Al)-based flocculants in the treatment of drinking water	119:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	7	57	theme	kaolin	1263:1268	arg1	suspension					1270:1279	the model kaolin suspension	1253:1279	the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units	1253:1344	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	0	58	theme	water	61:65	arg1	treatment					67:75	water treatment	61:75	water treatment	61:75	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.
31560145	10	59	theme	CMP	1849:1851	arg1	use					1842:1844	the solo use	1833:1844	the solo use of CMP or AlCl3	1833:1860	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	8	60	theme	flocculant	1543:1552	arg1	development					1494:1504	the development	1490:1504	the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants	1490:1623	The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants.
31560145	5	61	from	influence	854:862	arg1	neutralization					874:887	charge neutralization	867:887	charge neutralization	867:887	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	7	62	theme	zeta	1351:1354	arg1	-2.28 mV					1423:1430	-2.28 mV	1423:1430	-2.28 mV	1423:1430	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	62	theme	zeta	1351:1354	arg1	%					1440:1440	98.0%	1436:1440	98.0%	1436:1440	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	62	theme	zeta	1351:1354	arg1	activity					1395:1402	the maximum flocculating activity	1370:1402	the maximum flocculating activity	1370:1402	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	7	62	theme	zeta	1351:1354	arg1	potential					1356:1364	the zeta potential	1347:1364	the zeta potential	1347:1364	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	2	63	theme	kaolin	399:404	arg1	flocculation					383:394	the flocculation	379:394	the flocculation of kaolin	379:404	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	8	64	theme	water-dissolved	1568:1582	arg1	polysaccharide					1584:1597	water-dissolved polysaccharide	1568:1597	water-dissolved polysaccharide	1568:1597	The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants.
31560145	7	65	theme	nephelometric	1316:1328	arg1	units					1340:1344	50 nephelometric turbidity units	1313:1344	50 nephelometric turbidity units	1313:1344	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
31560145	9	66	theme	water	1730:1734	arg1	treatment					1736:1744	water treatment	1730:1744	water treatment	1730:1744	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	3	67	theme	coagulation	435:445	arg1	effective					498:506	effective	498:506	effective	498:506	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	3	67	theme	coagulation	435:445	arg1	efficiency					447:456	the coagulation efficiency	431:456	the coagulation efficiency of carboxymethylpullulan-AlCl3	431:487	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	3	68	from	turbidity	520:528	arg1	effective					498:506	effective	498:506	effective	498:506	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	3	68	from	turbidity	520:528	arg1	efficiency					447:456	the coagulation efficiency	431:456	the coagulation efficiency of carboxymethylpullulan-AlCl3	431:487	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	1	69	from	reduction	106:114	arg1	use					123:125	the use	119:125	the use of aluminum (Al)-based flocculants in the treatment of drinking water	119:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	6	70	theme	secondary	1166:1174	arg1	pollution					1188:1196	secondary environment pollution	1166:1196	secondary environment pollution	1166:1196	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	6	71	contain	possessed	1041:1049	arg1	flocculant					1030:1039	the composite flocculant	1016:1039	the composite flocculant	1016:1039	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	6	71	contain	possessed	1041:1049	arg2	advantages					1056:1065	both advantages	1051:1065	both advantages that enhanced flocculation, and decreased the dosage of AlCl3	1051:1127	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	1	72	theme	drinking	182:189	arg1	water					191:195	drinking water	182:195	drinking water	182:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	9	73	theme	composite	1655:1663	arg1	CMP-AlCl3					1676:1684	A novel composite flocculant CMP-AlCl3	1647:1684	A novel composite flocculant CMP-AlCl3	1647:1684	PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment.
31560145	10	74	from	effective	1796:1804	arg1	turbidity					1818:1826	reducing turbidity	1809:1826	reducing turbidity	1809:1826	The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 .
31560145	2	75	theme	composite	270:278	arg1	flocculant					280:289	a novel composite flocculant	262:289	a novel composite flocculant	262:289	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	1	76	theme	water	191:195	arg1	treatment					169:177	the treatment	165:177	the treatment of drinking water	165:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	2	77	dep	lab-scale	348:356	arg1	jar					359:361	jar	359:361	jar	359:361	In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin.
31560145	1	78	from	flocculants	150:160	arg1	treatment					169:177	the treatment	165:177	the treatment of drinking water	165:195	A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons.
31560145	3	79	theme	carboxymethylpullulan-AlCl3	461:487	arg1	effective					498:506	effective	498:506	effective	498:506	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	3	79	theme	carboxymethylpullulan-AlCl3	461:487	arg1	efficiency					447:456	the coagulation efficiency	431:456	the coagulation efficiency of carboxymethylpullulan-AlCl3	431:487	The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 .
31560145	6	80	theme	AlCl3	1123:1127	arg1	dosage					1113:1118	the dosage	1109:1118	the dosage of AlCl3	1109:1127	Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution.
31560145	4	81	theme	optimum	588:594	arg1	conditions					606:615	The optimum treatment conditions	584:615	The optimum treatment conditions assessed by a response surface methodology	584:658	The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 .
31560145	5	82	theme	Zeta	743:746	arg1	measurements					758:769	Zeta potential measurements	743:769	Zeta potential measurements	743:769	Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges.
31560145	0	83	dep	Evaluation	0:9	arg1	study					85:89	A case study	78:89	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.	0:102	Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.
31560145	7	84	theme	units	1340:1344	arg1	turbidity					1300:1308	a turbidity	1298:1308	a turbidity of 50 nephelometric turbidity units	1298:1344	When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively.
30015261	4	0	theme	S.	627:628	arg1	isolates					639:646	S. enterica isolates	627:646	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)]	627:816	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	10	1	theme	cellular	1693:1700	arg1	component					1702:1710	this cellular component	1688:1710	this cellular component	1688:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	15	2	theme	O-antigen	2430:2438	arg1	composition					2440:2450	O-antigen composition	2430:2450	O-antigen composition	2430:2450	Further studies on O-antigen composition would clarify the fundaments of discrimination obtained by FTIRS.
30015261	3	3	from	role	541:544	arg1	discrimination					593:606	the FTIRS Salmonella discrimination	572:606	the FTIRS Salmonella discrimination	572:606	Moreover, the role of O-units composition on the FTIRS Salmonella discrimination was also explored.
30015261	1	4	theme	pathogen	282:289	arg1	surveillance					242:253	epidemiologic surveillance	228:253	epidemiologic surveillance of this important zoonotic pathogen	228:289	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	3	5	theme	Salmonella	582:591	arg1	discrimination					593:606	the FTIRS Salmonella discrimination	572:606	the FTIRS Salmonella discrimination	572:606	Moreover, the role of O-units composition on the FTIRS Salmonella discrimination was also explored.
30015261	1	6	theme	Salmonella	184:193	arg1	typing					204:209	routine Salmonella enterica typing	176:209	routine Salmonella enterica typing	176:209	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	10	7	theme	serogroup	1563:1571	arg1	structure					1550:1558	O-unit structure	1543:1558	O-unit structure of serogroup H and K	1543:1579	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	5	8	theme	Least	884:888	arg1	Analysis					910:917	Partial Least Square Discriminant Analysis	876:917	Partial Least Square Discriminant Analysis (PLSDA)	876:925	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	8	theme	Least	884:888	arg1	PLSDA					920:924	PLSDA	920:924	PLSDA	920:924	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	8	9	theme	structures	1233:1242	arg1	composition					1244:1254	the O-unit structures composition	1222:1254	the O-unit structures composition of those serogroups	1222:1274	Differences in the O-unit structures composition of those serogroups are likely justifying the discrimination achieved.
30015261	4	10	dep	isolates	639:646	arg1	n = 325					649:655	n = 325	649:655	n = 325	649:655	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	5	11	theme	Infrared	842:849	arg1	spectra					851:857	Infrared spectra	842:857	Infrared spectra	842:857	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	1	12	theme	epidemiologic	228:240	arg1	surveillance					242:253	epidemiologic surveillance	228:253	epidemiologic surveillance of this important zoonotic pathogen	228:289	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	10	13	with	similarity	1592:1601	arg1	C1					1622:1623	sub-serogroup C1	1608:1623	sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component	1608:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	8	14	theme	serogroups	1265:1274	arg1	composition					1244:1254	the O-unit structures composition	1222:1254	the O-unit structures composition of those serogroups	1222:1274	Differences in the O-unit structures composition of those serogroups are likely justifying the discrimination achieved.
30015261	8	15	from	Differences	1207:1217	arg1	composition					1244:1254	the O-unit structures composition	1222:1254	the O-unit structures composition of those serogroups	1222:1274	Differences in the O-unit structures composition of those serogroups are likely justifying the discrimination achieved.
30015261	1	16	theme	Simpler	136:142	arg1	methods					164:170	Simpler, quick and low-cost methods	136:170	Simpler, quick and low-cost methods for routine Salmonella enterica typing	136:209	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	12	17	theme	Appropriate	1840:1850	arg1	discrimination					1861:1874	Appropriate serotype discrimination	1840:1874	Appropriate serotype discrimination	1840:1874	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	13	18	from	role	2130:2133	arg1	discrimination					2169:2182	the discrimination	2165:2182	the discrimination at serotype level obtained	2165:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	4	19	dep	n = 325	649:655	arg1	sources					679:685	different sources	669:685	different sources	669:685	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	19	dep	n = 325	649:655	arg1	C-n = 108					783:791	C-n = 108	783:791	C-n = 108	783:791	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	19	dep	n = 325	649:655	arg1	D-n = 43					794:801	D-n = 43	794:801	D-n = 43	794:801	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	19	dep	n = 325	649:655	arg1	countries					691:699	countries)	691:700	countries)	691:700	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	19	dep	n = 325	649:655	arg1	2002-2015					658:666	2002-2015	658:666	2002-2015	658:666	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	19	dep	n = 325	649:655	arg1	E-n = 33					807:814	E-n = 33	807:814	E-n = 33	807:814	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	10	20	theme	sub-serogroup	1608:1620	arg1	C1					1622:1623	sub-serogroup C1	1608:1623	sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component	1608:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	1	21	theme	quick	145:149	arg1	methods					164:170	Simpler, quick and low-cost methods	136:170	Simpler, quick and low-cost methods for routine Salmonella enterica typing	136:209	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	12	22	theme	-PLSDA	2023:2028	arg1	serotypes					2007:2015	the remaining D1/D2 serotypes	1987:2015	the remaining D1/D2 serotypes (93.4%-PLSDA)	1987:2029	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	22	theme	-PLSDA	2023:2028	arg1	%					2022:2022	93.4%-PLSDA	2018:2028	93.4%-PLSDA	2018:2028	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	4	23	theme	serotypes	709:717	arg1	sources					679:685	different sources	669:685	different sources	669:685	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	23	theme	serotypes	709:717	arg1	countries					691:699	countries)	691:700	countries)	691:700	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	9	24	dep	Cerro	1519:1523	arg1	n = 2					1526:1530	n = 2	1526:1530	n = 2	1526:1530	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	3	25	theme	FTIRS	576:580	arg1	discrimination					593:606	the FTIRS Salmonella discrimination	572:606	the FTIRS Salmonella discrimination	572:606	Moreover, the role of O-units composition on the FTIRS Salmonella discrimination was also explored.
30015261	5	26	theme	Discriminant	897:908	arg1	Analysis					910:917	Partial Least Square Discriminant Analysis	876:917	Partial Least Square Discriminant Analysis (PLSDA)	876:925	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	26	theme	Discriminant	897:908	arg1	PLSDA					920:924	PLSDA	920:924	PLSDA	920:924	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	6	27	theme	sharpest	1019:1026	arg1	differences					1028:1038	the spectral sharpest differences	1006:1038	the spectral sharpest differences being used in the subsequent Salmonella typing	1006:1085	The polysaccharides region provided the spectral sharpest differences being used in the subsequent Salmonella typing.
30015261	12	28	theme	remaining	1991:1999	arg1	serotypes					2007:2015	the remaining D1/D2 serotypes	1987:2015	the remaining D1/D2 serotypes (93.4%-PLSDA)	1987:2029	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	28	theme	remaining	1991:1999	arg1	%					2022:2022	93.4%-PLSDA	2018:2028	93.4%-PLSDA	2018:2028	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	0	29	theme	Infrared	87:94	arg1	Spectroscopy					96:107	Infrared Spectroscopy	87:107	Infrared Spectroscopy	87:107	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	15	30	theme	discrimination	2484:2497	arg1	fundaments					2470:2479	the fundaments	2466:2479	the fundaments of discrimination obtained by FTIRS	2466:2515	Further studies on O-antigen composition would clarify the fundaments of discrimination obtained by FTIRS.
30015261	13	31	theme	role	2130:2133	arg1	elucidation					2111:2121	the elucidation	2107:2121	the elucidation of the role of this cellular component on the discrimination at serotype level obtained	2107:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	7	32	theme	assignments	1180:1190	arg1	%					1167:1167	99.6%	1163:1167	99.6% of correct assignments; PLSDA model	1163:1203	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	32	theme	assignments	1180:1190	arg1	assignments					1180:1190	correct assignments	1172:1190	correct assignments	1172:1190	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	5	33	theme	Principal	934:942	arg1	PCA					964:966	PCA	964:966	PCA	964:966	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	33	theme	Principal	934:942	arg1	Analysis					954:961	Principal Component Analysis	934:961	Principal Component Analysis (PCA)	934:967	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	2	34	dep	transform	390:398	arg1	infrared					400:407	infrared	400:407	transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella	390:524	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	0	35	theme	comprehensive	112:124	arg1	analysis					126:133	A comprehensive analysis	110:133	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.	0:134	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	13	36	theme	component	2152:2160	arg1	role					2130:2133	the role	2126:2133	the role of this cellular component on the discrimination at serotype level obtained	2126:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	12	37	theme	-PLSDA	1949:1954	arg1	serotypes					1933:1941	the remaining C1 serotypes	1916:1941	the remaining C1 serotypes (91.5%-PLSDA)	1916:1955	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	37	theme	-PLSDA	1949:1954	arg1	%					1948:1948	91.5%-PLSDA	1944:1954	91.5%-PLSDA	1944:1954	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	9	38	dep	Sundsvall	1491:1499	arg1	n = 1					1502:1506	n = 1	1502:1506	n = 1	1502:1506	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	14	39	dep	serogroups	2252:2261	arg1	C					2267:2267	C	2267:2267	C	2267:2267	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	39	dep	serogroups	2252:2261	arg1	serogroups					2252:2261	relevant serogroups	2243:2261	relevant serogroups (B, C, D and E)	2243:2277	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	39	dep	serogroups	2252:2261	arg1	B					2264:2264	B	2264:2264	B	2264:2264	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	39	dep	serogroups	2252:2261	arg1	E					2276:2276	E	2276:2276	E	2276:2276	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	39	dep	serogroups	2252:2261	arg1	D					2270:2270	D	2270:2270	D	2270:2270	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	8	40	theme	O-unit	1226:1231	arg1	structures					1233:1242	O-unit structures	1226:1242	the O-unit structures composition of those serogroups	1222:1274	Differences in the O-unit structures composition of those serogroups are likely justifying the discrimination achieved.
30015261	2	41	theme	clinically-relevant	455:473	arg1	serogroups					475:484	the most clinically-relevant serogroups	446:484	the most clinically-relevant serogroups	446:484	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	0	42	theme	non-typhoid	18:28	arg1	serogroups					41:50	serogroups	41:50	serogroups	41:50	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	0	43	dep	Fourier	69:75	arg1	Transform					77:85	Transform	77:85	Transform Infrared Spectroscopy	77:107	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	6	44	theme	subsequent	1058:1067	arg1	typing					1080:1085	the subsequent Salmonella typing	1054:1085	the subsequent Salmonella typing	1054:1085	The polysaccharides region provided the spectral sharpest differences being used in the subsequent Salmonella typing.
30015261	2	45	theme	Salmonella	515:524	arg1	serogroups					475:484	the most clinically-relevant serogroups	446:484	the most clinically-relevant serogroups	446:484	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	2	45	theme	Salmonella	515:524	arg1	serotypes					490:498	serotypes	490:498	serotypes of non-typhoid Salmonella	490:524	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	10	46	theme	remaining	1634:1642	arg1	serogroups					1644:1653	the remaining serogroups	1630:1653	the remaining serogroups presenting marked differences in this cellular component	1630:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	0	47	theme	serogroups	41:50	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.	0:134	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	14	48	theme	relevant	2243:2250	arg1	C					2267:2267	C	2267:2267	C	2267:2267	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	48	theme	relevant	2243:2250	arg1	E					2276:2276	E	2276:2276	E	2276:2276	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	48	theme	relevant	2243:2250	arg1	B					2264:2264	B	2264:2264	B	2264:2264	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	48	theme	relevant	2243:2250	arg1	serogroups					2252:2261	relevant serogroups	2243:2261	relevant serogroups (B, C, D and E)	2243:2277	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	48	theme	relevant	2243:2250	arg1	D					2270:2270	D	2270:2270	D	2270:2270	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	15	49	from	studies	2419:2425	arg1	composition					2440:2450	O-antigen composition	2430:2450	O-antigen composition	2430:2450	Further studies on O-antigen composition would clarify the fundaments of discrimination obtained by FTIRS.
30015261	7	50	dep	%	1167:1167	arg1	model					1199:1203	PLSDA model	1193:1203	99.6% of correct assignments; PLSDA model	1163:1203	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	0	51	theme	serotypes	56:64	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.	0:134	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	9	52	dep	serogroups	1333:1342	arg1	L					1354:1354	L	1354:1354	L	1354:1354	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	O					1363:1363	O	1363:1363	O	1363:1363	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	G					1345:1345	G	1345:1345	G	1345:1345	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	T					1366:1366	T	1366:1366	T	1366:1366	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	Z					1375:1375	Z	1375:1375	Z	1375:1375	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	H					1348:1348	H	1348:1348	H	1348:1348	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	M					1357:1357	M	1357:1357	M	1357:1357	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	N					1360:1360	N	1360:1360	N	1360:1360	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	U					1369:1369	U	1369:1369	U	1369:1369	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	K					1351:1351	K	1351:1351	K	1351:1351	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	52	dep	serogroups	1333:1342	arg1	Y					1372:1372	Y	1372:1372	Y	1372:1372	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	0	53	dep	serogroups	41:50	arg1	Salmonella					30:39	non-typhoid Salmonella serogroups and serotypes	18:64	non-typhoid Salmonella serogroups and serotypes	18:64	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	14	54	theme	particular	2332:2341	arg1	serotypes					2353:2361	particular important serotypes	2332:2361	particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg)	2332:2408	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	54	theme	particular	2332:2341	arg1	Rissen					2383:2388	Rissen	2383:2388	Rissen	2383:2388	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	54	theme	particular	2332:2341	arg1	Senftenberg					2397:2407	Senftenberg	2397:2407	Senftenberg	2397:2407	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	13	55	theme	O-unit	2054:2059	arg1	composition					2061:2071	available O-unit composition	2044:2071	available O-unit composition	2044:2071	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	1	56	theme	low-cost	155:162	arg1	methods					164:170	Simpler, quick and low-cost methods	136:170	Simpler, quick and low-cost methods for routine Salmonella enterica typing	136:209	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	10	57	dep	serogroup	1563:1571	arg1	K					1579:1579	K	1579:1579	K	1579:1579	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	10	57	dep	serogroup	1563:1571	arg1	H					1573:1573	H	1573:1573	H	1573:1573	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	12	58	theme	remaining	1920:1928	arg1	serotypes					1933:1941	the remaining C1 serotypes	1916:1941	the remaining C1 serotypes (91.5%-PLSDA)	1916:1955	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	58	theme	remaining	1920:1928	arg1	%					1948:1948	91.5%-PLSDA	1944:1954	91.5%-PLSDA	1944:1954	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	3	59	theme	O-units	549:555	arg1	composition					557:567	O-units composition	549:567	O-units composition	549:567	Moreover, the role of O-units composition on the FTIRS Salmonella discrimination was also explored.
30015261	9	60	theme	serogroups	1474:1483	arg1	isolates					1462:1469	3 isolates	1460:1469	3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2)	1460:1531	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	7	61	dep	Serogroups	1088:1097	arg1	C					1103:1103	C	1103:1103	C	1103:1103	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	61	dep	Serogroups	1088:1097	arg1	Serogroups					1088:1097	Serogroups	1088:1097	Serogroups (B, C, D and E) discrimination	1088:1128	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	61	dep	Serogroups	1088:1097	arg1	B					1100:1100	B	1100:1100	B	1100:1100	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	61	dep	Serogroups	1088:1097	arg1	E					1112:1112	E	1112:1112	E	1112:1112	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	61	dep	Serogroups	1088:1097	arg1	D					1106:1106	D	1106:1106	D	1106:1106	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	1	62	theme	zoonotic	273:280	arg1	pathogen					282:289	this important zoonotic pathogen	258:289	this important zoonotic pathogen	258:289	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	15	63	theme	Further	2411:2417	arg1	studies					2419:2425	Further studies	2411:2425	Further studies on O-antigen composition	2411:2450	Further studies on O-antigen composition would clarify the fundaments of discrimination obtained by FTIRS.
30015261	10	64	theme	O-unit	1543:1548	arg1	structure					1550:1558	O-unit structure	1543:1558	O-unit structure of serogroup H and K	1543:1579	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	4	65	theme	enterica	630:637	arg1	isolates					639:646	S. enterica isolates	627:646	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)]	627:816	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	1	66	theme	routine	176:182	arg1	typing					204:209	routine Salmonella enterica typing	176:209	routine Salmonella enterica typing	176:209	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	10	67	from	differences	1673:1683	arg1	component					1702:1710	this cellular component	1688:1710	this cellular component	1688:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	14	68	dep	serotypes	2353:2361	arg1	serotypes					2353:2361	particular important serotypes	2332:2361	particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg)	2332:2408	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	68	dep	serotypes	2353:2361	arg1	Rissen					2383:2388	Rissen	2383:2388	Rissen	2383:2388	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	68	dep	serotypes	2353:2361	arg1	Senftenberg					2397:2407	Senftenberg	2397:2407	Senftenberg	2397:2407	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	7	69	theme	PLSDA	1193:1197	arg1	model					1199:1203	PLSDA model	1193:1203	99.6% of correct assignments; PLSDA model	1163:1203	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	5	70	theme	Partial	876:882	arg1	Analysis					910:917	Partial Least Square Discriminant Analysis	876:917	Partial Least Square Discriminant Analysis (PLSDA)	876:925	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	70	theme	Partial	876:882	arg1	PLSDA					920:924	PLSDA	920:924	PLSDA	920:924	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	6	71	theme	polysaccharides	974:988	arg1	region					990:995	The polysaccharides region	970:995	The polysaccharides region	970:995	The polysaccharides region provided the spectral sharpest differences being used in the subsequent Salmonella typing.
30015261	7	72	theme	Serogroups	1088:1097	arg1	discrimination					1115:1128	Serogroups (B, C, D and E) discrimination	1088:1128	Serogroups (B, C, D and E) discrimination	1088:1128	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	2	73	theme	comprehensive	315:327	arg1	collection					337:346	a comprehensive isolate collection	313:346	a comprehensive isolate collection	313:346	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	2	74	theme	isolate	329:335	arg1	collection					337:346	a comprehensive isolate collection	313:346	a comprehensive isolate collection	313:346	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	12	75	theme	serotype	1852:1859	arg1	discrimination					1861:1874	Appropriate serotype discrimination	1840:1874	Appropriate serotype discrimination	1840:1874	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	10	76	with	C1	1622:1623	arg1	serogroups					1644:1653	the remaining serogroups	1630:1653	the remaining serogroups presenting marked differences in this cellular component	1630:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	13	77	theme	serotype	2187:2194	arg1	level					2196:2200	serotype level	2187:2200	serotype level obtained	2187:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	4	78	theme	serogroups	726:735	arg1	sources					679:685	different sources	669:685	different sources	669:685	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	4	78	theme	serogroups	726:735	arg1	countries					691:699	countries)	691:700	countries)	691:700	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	13	79	from	discrimination	2169:2182	arg1	elucidation					2111:2121	the elucidation	2107:2121	the elucidation of the role of this cellular component on the discrimination at serotype level obtained	2107:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	9	80	dep	serogroups	1423:1432	arg1	B					1434:1434	B	1434:1434	B	1434:1434	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	80	dep	serogroups	1423:1432	arg1	E					1446:1446	E	1446:1446	E	1446:1446	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	80	dep	serogroups	1423:1432	arg1	D					1440:1440	D	1440:1440	D	1440:1440	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	80	dep	serogroups	1423:1432	arg1	C					1437:1437	C	1437:1437	C	1437:1437	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	13	81	from	elucidation	2111:2121	arg1	discrimination					2169:2182	the discrimination	2165:2182	the discrimination at serotype level obtained	2165:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	4	82	theme	different	669:677	arg1	sources					679:685	different sources	669:685	different sources	669:685	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	5	83	theme	Square	890:895	arg1	Analysis					910:917	Partial Least Square Discriminant Analysis	876:917	Partial Least Square Discriminant Analysis (PLSDA)	876:925	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	83	theme	Square	890:895	arg1	PLSDA					920:924	PLSDA	920:924	PLSDA	920:924	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	12	84	theme	D1/D2	2001:2005	arg1	serotypes					2007:2015	the remaining D1/D2 serotypes	1987:2015	the remaining D1/D2 serotypes (93.4%-PLSDA)	1987:2029	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	84	theme	D1/D2	2001:2005	arg1	%					2022:2022	93.4%-PLSDA	2018:2028	93.4%-PLSDA	2018:2028	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	6	85	theme	spectral	1010:1017	arg1	differences					1028:1038	the spectral sharpest differences	1006:1038	the spectral sharpest differences being used in the subsequent Salmonella typing	1006:1085	The polysaccharides region provided the spectral sharpest differences being used in the subsequent Salmonella typing.
30015261	7	86	dep	accuracy	1153:1160	arg1	%					1167:1167	99.6%	1163:1167	99.6% of correct assignments; PLSDA model	1163:1203	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	86	dep	accuracy	1153:1160	arg1	assignments					1180:1190	correct assignments	1172:1190	correct assignments	1172:1190	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	7	87	theme	correct	1172:1178	arg1	assignments					1180:1190	correct assignments	1172:1190	correct assignments	1172:1190	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	9	88	dep	serogroups	1474:1483	arg1	H					1485:1485	H	1485:1485	H (S. Sundsvall, n = 1)	1485:1507	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	88	dep	serogroups	1474:1483	arg1	Sundsvall					1491:1499	Sundsvall	1491:1499	Sundsvall	1491:1499	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	88	dep	serogroups	1474:1483	arg1	Cerro					1519:1523	Cerro	1519:1523	Cerro	1519:1523	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	88	dep	serogroups	1474:1483	arg1	K					1513:1513	K	1513:1513	K (S. Cerro, n = 2)	1513:1531	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	9	88	dep	serogroups	1474:1483	arg1	serogroups					1474:1483	serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2)	1474:1531	serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2)	1474:1531	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	13	89	theme	cellular	2143:2150	arg1	component					2152:2160	this cellular component	2138:2160	this cellular component	2138:2160	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	13	90	from	level	2196:2200	arg1	discrimination					2169:2182	the discrimination	2165:2182	the discrimination at serotype level obtained	2165:2209	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	2	91	dep	Fourier	382:388	arg1	transform					390:398	transform	390:398	transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella	390:524	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	5	92	theme	Component	944:952	arg1	PCA					964:966	PCA	964:966	PCA	964:966	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	5	92	theme	Component	944:952	arg1	Analysis					954:961	Principal Component Analysis	934:961	Principal Component Analysis (PCA)	934:967	Infrared spectra were analysed by Partial Least Square Discriminant Analysis (PLSDA) and/or Principal Component Analysis (PCA).
30015261	14	93	dep	sub-serogroups	2280:2293	arg1	C2					2300:2301	C2	2300:2301	C2	2300:2301	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	93	dep	sub-serogroups	2280:2293	arg1	C1					2296:2297	C1	2296:2297	C1	2296:2297	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	93	dep	sub-serogroups	2280:2293	arg1	sub-serogroups					2280:2293	sub-serogroups	2280:2293	sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4)	2280:2326	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	93	dep	sub-serogroups	2280:2293	arg1	C3					2307:2308	C3	2307:2308	C3	2307:2308	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	4	94	theme	frequent	757:764	arg1	ones					766:769	the most frequent ones	748:769	the most frequent ones	748:769	S. enterica isolates (n = 325; 2002-2015; different sources and countries), of 57 serotypes and 15 serogroups [including the most frequent ones, B-n = 122; C-n = 108; D-n = 43 and E-n = 33)] were analysed by FTIRS.
30015261	7	95	theme	high	1148:1151	arg1	accuracy					1153:1160	high accuracy	1148:1160	high accuracy (99.6% of correct assignments; PLSDA model)	1148:1204	Serogroups (B, C, D and E) discrimination was achieved with high accuracy (99.6% of correct assignments; PLSDA model).
30015261	9	96	theme	Other	1327:1331	arg1	serogroups					1333:1342	Other serogroups	1327:1342	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z)	1327:1376	Other serogroups (G, H, K, L, M, N, O, T, U, Y, Z) were correctly predicted as not belonging to serogroups B, C, D nor E, except for 3 isolates of serogroups H (S. Sundsvall, n = 1) and K (S. Cerro, n = 2).
30015261	6	97	theme	Salmonella	1069:1078	arg1	typing					1080:1085	the subsequent Salmonella typing	1054:1085	the subsequent Salmonella typing	1054:1085	The polysaccharides region provided the spectral sharpest differences being used in the subsequent Salmonella typing.
30015261	2	98	theme	most	450:453	arg1	serogroups					475:484	the most clinically-relevant serogroups	446:484	the most clinically-relevant serogroups	446:484	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	14	99	dep	C1	2296:2297	arg1	E4					2324:2325	E4	2324:2325	E4	2324:2325	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	99	dep	C1	2296:2297	arg1	E1-E2-E3					2311:2318	E1-E2-E3	2311:2318	E1-E2-E3	2311:2318	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	13	100	theme	particular	2077:2086	arg1	serotypes					2088:2096	particular serotypes	2077:2096	particular serotypes	2077:2096	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	12	101	theme	C1	1930:1931	arg1	serotypes					1933:1941	the remaining C1 serotypes	1916:1941	the remaining C1 serotypes (91.5%-PLSDA)	1916:1955	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	12	101	theme	C1	1930:1931	arg1	%					1948:1948	91.5%-PLSDA	1944:1954	91.5%-PLSDA	1944:1954	Appropriate serotype discrimination was obtained for most of S. Rissen from the remaining C1 serotypes (91.5%-PLSDA), and S. Enteritidis (D1) from the remaining D1/D2 serotypes (93.4%-PLSDA).
30015261	11	102	theme	sub-serogroups	1717:1730	arg1	discrimination					1732:1745	The sub-serogroups discrimination	1713:1745	The sub-serogroups discrimination	1713:1745	The sub-serogroups discrimination was successfully achieved for C1, C2 and C3 (using PCA), and for E1-E2-E3 and E4 (by PLSDA).
30015261	14	103	theme	important	2343:2351	arg1	serotypes					2353:2361	particular important serotypes	2332:2361	particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg)	2332:2408	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	103	theme	important	2343:2351	arg1	Rissen					2383:2388	Rissen	2383:2388	Rissen	2383:2388	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	14	103	theme	important	2343:2351	arg1	Senftenberg					2397:2407	Senftenberg	2397:2407	Senftenberg	2397:2407	FTIRS was able to discriminate relevant serogroups (B, C, D and E), sub-serogroups (C1, C2 and C3; E1-E2-E3 and E4) and particular important serotypes (S. Enteritidis, S. Rissen and S. Senftenberg).
30015261	1	104	dep	Salmonella	184:193	arg1	enterica					195:202	enterica	195:202	enterica	195:202	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30015261	0	105	dep	Discrimination	0:13	arg1	analysis					126:133	A comprehensive analysis	110:133	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.	0:134	Discrimination of non-typhoid Salmonella serogroups and serotypes by Fourier Transform Infrared Spectroscopy: A comprehensive analysis.
30015261	13	106	theme	available	2044:2052	arg1	composition					2061:2071	available O-unit composition	2044:2071	available O-unit composition	2044:2071	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	10	107	theme	marked	1666:1671	arg1	differences					1673:1683	marked differences	1666:1683	marked differences in this cellular component	1666:1710	In fact, O-unit structure of serogroup H and K shows some similarity with sub-serogroup C1 with the remaining serogroups presenting marked differences in this cellular component.
30015261	2	108	theme	Fourier	382:388	arg1	potential					369:377	the potential	365:377	the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella	365:524	In this study, using a comprehensive isolate collection, we investigated the potential of Fourier transform infrared spectroscopy (FTIRS) to discriminate the most clinically-relevant serogroups and serotypes of non-typhoid Salmonella.
30015261	13	109	theme	composition	2061:2071	arg1	lack					2036:2039	The lack	2032:2039	The lack of available O-unit composition for particular serotypes	2032:2096	The lack of available O-unit composition for particular serotypes prevents the elucidation of the role of this cellular component on the discrimination at serotype level obtained.
30015261	3	110	theme	composition	557:567	arg1	role					541:544	the role	537:544	the role of O-units composition on the FTIRS Salmonella discrimination	537:606	Moreover, the role of O-units composition on the FTIRS Salmonella discrimination was also explored.
30015261	1	111	theme	important	263:271	arg1	pathogen					282:289	this important zoonotic pathogen	258:289	this important zoonotic pathogen	258:289	Simpler, quick and low-cost methods for routine Salmonella enterica typing are required for epidemiologic surveillance of this important zoonotic pathogen.
30989271	3	0	theme	substitution	748:759	arg1	degree					738:743	degree	738:743	degree of substitution	738:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	0	theme	substitution	748:759	arg1	mass					729:732	molar mass	723:732	molar mass	723:732	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	4	1	theme	reversed	910:917	arg1	column					928:933	a reversed phase C8 column	908:933	a reversed phase C8 column	908:933	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	5	2	theme	exclusion	1042:1050	arg1	separation					1068:1077	size exclusion chromatographic separation	1037:1077	size exclusion chromatographic separation of the fractions according to molar mass	1037:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	1	3	theme	various	192:198	arg1	applications					241:252	various pharmaceutical, biomedical, and cosmetic applications	192:252	various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties	192:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	0	4	theme	acid	109:112	arg1	characterization					60:75	the characterization	56:75	the characterization of acrylate-modified hyaluronic acid	56:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	1	5	theme	pharmaceutical	200:213	arg1	applications					241:252	various pharmaceutical, biomedical, and cosmetic applications	192:252	various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties	192:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	8	6	theme	complex	1450:1456	arg1	nature					1458:1463	the complex nature	1446:1463	the complex nature of hyaluronic acid and its derivatives	1446:1502	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	6	7	theme	acid	1154:1157	arg1	derivatives					1159:1169	the hyaluronic acid derivatives	1139:1169	the hyaluronic acid derivatives	1139:1169	It was found that the hyaluronic acid derivatives were broadly distributed with regard to both chemical composition and molar mass.
30989271	3	8	theme	degree	738:743	arg1	terms					714:718	terms	714:718	terms of molar mass and degree of substitution	714:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	4	9	theme	C8	925:926	arg1	column					928:933	a reversed phase C8 column	908:933	a reversed phase C8 column	908:933	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	8	10	theme	derivatives	1492:1502	arg1	nature					1458:1463	the complex nature	1446:1463	the complex nature of hyaluronic acid and its derivatives	1446:1502	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	3	11	theme	approach	612:619	arg1	development					528:538	the development	524:538	the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	524:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	12	theme	hyaluronic	637:646	arg1	acid					648:651	hyaluronic acid	637:651	hyaluronic acid	637:651	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	4	13	theme	phase	919:923	arg1	column					928:933	a reversed phase C8 column	908:933	a reversed phase C8 column	908:933	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	2	14	theme	molar	430:434	arg1	degree					467:472	degree	467:472	degree of substitution	467:488	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	2	14	theme	molar	430:434	arg1	mass					436:439	molar mass	430:439	molar mass	430:439	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	8	15	theme	hyaluronic	1468:1477	arg1	acid					1479:1482	hyaluronic acid	1468:1482	hyaluronic acid	1468:1482	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	3	16	theme	comprehensive	545:557	arg1	approach					612:619	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach	543:619	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	543:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	4	17	theme	solvent	887:893	arg1	gradient					895:902	a stepwise solvent gradient	876:902	a stepwise solvent gradient	876:902	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	6	18	theme	molar	1241:1245	arg1	mass					1247:1250	molar mass	1241:1250	molar mass	1241:1250	It was found that the hyaluronic acid derivatives were broadly distributed with regard to both chemical composition and molar mass.
30989271	8	19	theme	comprehensive	1393:1405	arg1	approach					1427:1434	a powerful approach	1416:1434	a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives	1416:1502	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	8	19	theme	comprehensive	1393:1405	arg1	2D-LC					1407:1411	comprehensive 2D-LC	1393:1411	comprehensive 2D-LC	1393:1411	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	4	20	theme	stepwise	878:885	arg1	gradient					895:902	a stepwise solvent gradient	876:902	a stepwise solvent gradient	876:902	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	5	21	from	dimension	961:969	arg1	Fractions					936:944	Fractions	936:944	Fractions from the first dimension	936:969	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	5	22	theme	size	1037:1040	arg1	exclusion					1042:1050	size exclusion	1037:1050	size exclusion chromatographic separation of the fractions according to molar mass	1037:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	6	23	theme	hyaluronic	1143:1152	arg1	derivatives					1159:1169	the hyaluronic acid derivatives	1139:1169	the hyaluronic acid derivatives	1139:1169	It was found that the hyaluronic acid derivatives were broadly distributed with regard to both chemical composition and molar mass.
30989271	1	24	theme	biomedical	216:225	arg1	applications					241:252	various pharmaceutical, biomedical, and cosmetic applications	192:252	various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties	192:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	0	25	theme	two-dimensional	14:28	arg1	chromatography					37:50	Comprehensive two-dimensional liquid chromatography	0:50	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid	0:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	7	26	theme	substitution	1289:1300	arg1	degrees					1278:1284	different degrees	1268:1284	different degrees of substitution	1268:1300	Fractions with different degrees of substitution were identified, and their molar mass distributions were determined.
30989271	1	27	theme	Hyaluronic	115:124	arg1	acid					126:129	Hyaluronic acid	115:129	Hyaluronic acid	115:129	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	27	theme	Hyaluronic	115:124	arg1	derivatives					148:158	its acrylate derivatives	135:158	its acrylate derivatives	135:158	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	27	theme	Hyaluronic	115:124	arg1	intermediates					174:186	important intermediates	164:186	important intermediates	164:186	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	5	28	theme	first	955:959	arg1	dimension					961:969	the first dimension	951:969	the first dimension	951:969	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	3	29	dep	derivatives	670:680	arg1	DS					683:684	DS	683:684	DS ranging from 0.4 to 3.1	683:708	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	0	30	theme	Comprehensive	0:12	arg1	chromatography					37:50	Comprehensive two-dimensional liquid chromatography	0:50	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid	0:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	5	31	theme	chromatographic	1052:1066	arg1	separation					1068:1077	size exclusion chromatographic separation	1037:1077	size exclusion chromatographic separation of the fractions according to molar mass	1037:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	2	32	theme	chemical	445:452	arg1	composition					454:464	chemical composition	445:464	chemical composition	445:464	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	8	33	theme	acid	1479:1482	arg1	nature					1458:1463	the complex nature	1446:1463	the complex nature of hyaluronic acid and its derivatives	1446:1502	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	4	34	theme	2D-LC	792:796	arg1	method					798:803	the 2D-LC method	788:803	the 2D-LC method	788:803	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	7	35	with	Fractions	1253:1261	arg1	degrees					1278:1284	different degrees	1268:1284	different degrees of substitution	1268:1300	Fractions with different degrees of substitution were identified, and their molar mass distributions were determined.
30989271	1	36	theme	cosmetic	232:239	arg1	applications					241:252	various pharmaceutical, biomedical, and cosmetic applications	192:252	various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties	192:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	0	37	theme	liquid	30:35	arg1	chromatography					37:50	Comprehensive two-dimensional liquid chromatography	0:50	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid	0:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	3	38	theme	liquid	582:587	arg1	2D-LC					605:609	2D-LC	605:609	2D-LC	605:609	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	38	theme	liquid	582:587	arg1	chromatography					589:602	online two-dimensional liquid chromatography	559:602	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	543:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	6	39	theme	chemical	1216:1223	arg1	composition					1225:1235	chemical composition	1216:1235	chemical composition	1216:1235	It was found that the hyaluronic acid derivatives were broadly distributed with regard to both chemical composition and molar mass.
30989271	3	40	theme	two-dimensional	566:580	arg1	2D-LC					605:609	2D-LC	605:609	2D-LC	605:609	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	40	theme	two-dimensional	566:580	arg1	chromatography					589:602	online two-dimensional liquid chromatography	559:602	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	543:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	41	theme	online	559:564	arg1	2D-LC					605:609	2D-LC	605:609	2D-LC	605:609	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	41	theme	online	559:564	arg1	chromatography					589:602	online two-dimensional liquid chromatography	559:602	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	543:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	1	42	theme	due	254:256	arg1	applications					241:252	various pharmaceutical, biomedical, and cosmetic applications	192:252	various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties	192:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	5	43	theme	molar	1109:1113	arg1	mass					1115:1118	molar mass	1109:1118	molar mass	1109:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	2	44	theme	substitution	477:488	arg1	degree					467:472	degree	467:472	degree of substitution	467:488	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	2	44	theme	substitution	477:488	arg1	mass					436:439	molar mass	430:439	molar mass	430:439	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	4	45	theme	method	798:803	arg1	dimension					775:783	the first dimension	765:783	the first dimension of the 2D-LC method	765:803	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	5	46	theme	fractions	1086:1094	arg1	separation					1068:1077	size exclusion chromatographic separation	1037:1077	size exclusion chromatographic separation of the fractions according to molar mass	1037:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	3	47	dep	0.4	699:701	arg1	to					703:704	to	703:704	to	703:704	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	7	48	theme	different	1268:1276	arg1	degrees					1278:1284	different degrees	1268:1284	different degrees of substitution	1268:1300	Fractions with different degrees of substitution were identified, and their molar mass distributions were determined.
30989271	8	49	theme	powerful	1418:1425	arg1	approach					1427:1434	a powerful approach	1416:1434	a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives	1416:1502	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	8	49	theme	powerful	1418:1425	arg1	2D-LC					1407:1411	comprehensive 2D-LC	1393:1411	comprehensive 2D-LC	1393:1411	The study proved that comprehensive 2D-LC is a powerful approach to reveal the complex nature of hyaluronic acid and its derivatives.
30989271	1	50	dep	biocompatibility	267:282	arg1	properties					304:313	properties	304:313	properties	304:313	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	9	51	dep	abstract	1515:1522	arg1	Graphical					1505:1513	Graphical	1505:1513	Graphical	1505:1513	Graphical abstract.
30989271	1	52	theme	acrylate	139:146	arg1	acid					126:129	Hyaluronic acid	115:129	Hyaluronic acid	115:129	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	52	theme	acrylate	139:146	arg1	derivatives					148:158	its acrylate derivatives	135:158	its acrylate derivatives	135:158	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	52	theme	acrylate	139:146	arg1	intermediates					174:186	important intermediates	164:186	important intermediates	164:186	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	3	53	theme	molar	723:727	arg1	mass					729:732	molar mass	723:732	molar mass	723:732	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	54	theme	acrylate	661:668	arg1	derivatives					670:680	its acrylate derivatives	657:680	its acrylate derivatives (DS ranging from 0.4 to 3.1)	657:709	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	2	55	theme	significant	394:404	arg1	heterogeneity					406:418	their significant heterogeneity	388:418	their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS)	388:493	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	3	56	theme	mass	729:732	arg1	terms					714:718	terms	714:718	terms of molar mass and degree of substitution	714:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	4	57	theme	chemical	830:837	arg1	composition/DS					839:852	chemical composition/DS	830:852	chemical composition/DS	830:852	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	4	58	theme	first	769:773	arg1	dimension					775:783	the first dimension	765:783	the first dimension of the 2D-LC method	765:803	In the first dimension of the 2D-LC method, separation according to chemical composition/DS was achieved by using a stepwise solvent gradient and a reversed phase C8 column.
30989271	5	59	theme	second	1009:1014	arg1	dimension					1016:1024	the second dimension	1005:1024	the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass	1005:1118	Fractions from the first dimension were automatically transferred to the second dimension comprising size exclusion chromatographic separation of the fractions according to molar mass.
30989271	1	60	theme	important	164:172	arg1	acid					126:129	Hyaluronic acid	115:129	Hyaluronic acid	115:129	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	60	theme	important	164:172	arg1	derivatives					148:158	its acrylate derivatives	135:158	its acrylate derivatives	135:158	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	1	60	theme	important	164:172	arg1	intermediates					174:186	important intermediates	164:186	important intermediates	164:186	Hyaluronic acid and its acrylate derivatives are important intermediates for various pharmaceutical, biomedical, and cosmetic applications due to their biocompatibility and viscoelasticity properties.
30989271	7	61	theme	mass	1335:1338	arg1	distributions					1340:1352	their molar mass distributions	1323:1352	their molar mass distributions	1323:1352	Fractions with different degrees of substitution were identified, and their molar mass distributions were determined.
30989271	0	62	theme	hyaluronic	98:107	arg1	acid					109:112	acrylate-modified hyaluronic acid	80:112	acrylate-modified hyaluronic acid	80:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	2	63	dep	degree	467:472	arg1	DS					491:492	DS	491:492	DS	491:492	However, these polymers are inherently difficult to characterize due to their significant heterogeneity regarding molar mass and chemical composition (degree of substitution, DS).
30989271	3	64	theme	chromatography	589:602	arg1	approach					612:619	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach	543:619	a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution	543:759	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	3	65	theme	present	500:506	arg1	study					508:512	The present study	496:512	The present study	496:512	The present study describes the development of a comprehensive online two-dimensional liquid chromatography (2D-LC) approach to characterize hyaluronic acid and its acrylate derivatives (DS ranging from 0.4 to 3.1) in terms of molar mass and degree of substitution.
30989271	0	66	theme	acrylate-modified	80:96	arg1	acid					109:112	acrylate-modified hyaluronic acid	80:112	acrylate-modified hyaluronic acid	80:112	Comprehensive two-dimensional liquid chromatography for the characterization of acrylate-modified hyaluronic acid.
30989271	7	67	theme	molar	1329:1333	arg1	distributions					1340:1352	their molar mass distributions	1323:1352	their molar mass distributions	1323:1352	Fractions with different degrees of substitution were identified, and their molar mass distributions were determined.
31429461	2	0	theme	pore-forming	471:482	arg1	agent					484:488	the pore-forming agent	467:488	the pore-forming agent	467:488	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	0	theme	pore-forming	471:482	arg1	agarose					456:462	agarose	456:462	agarose	456:462	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	5	1	theme	excellent	1050:1058	arg1	selectivity					1071:1081	excellent adsorption selectivity	1050:1081	excellent adsorption selectivity for BHb	1050:1089	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	6	2	theme	great	1242:1246	arg1	potential					1260:1268	great application potential	1242:1268	great application potential	1242:1268	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	5	3	theme	PCMs	807:810	arg1	application					792:802	the application	788:802	the application of PCMs as a protein adsorbent	788:833	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	4	theme	resultant	857:865	arg1	affinity					884:891	the resultant immobilized Cu2+ affinity adsorbent	853:901	the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15)	853:925	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	6	5	theme	adsorbent	1206:1214	arg1	affinity					1197:1204	the as-prepared affinity	1181:1204	the as-prepared affinity adsorbent with high performance	1181:1236	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	6	6	theme	bioseparation	1286:1298	arg1	field					1277:1281	the field	1273:1281	the field of bioseparation	1273:1298	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	5	7	theme	adsorption	1060:1069	arg1	selectivity					1071:1081	excellent adsorption selectivity	1050:1081	excellent adsorption selectivity for BHb	1050:1089	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	8	theme	immobilized	867:877	arg1	affinity					884:891	the resultant immobilized Cu2+ affinity adsorbent	853:901	the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15)	853:925	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	6	9	theme	biomass	1151:1157	arg1	chitosan					1159:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	4	10	theme	maximum	754:760	arg1	SSA					762:764	a maximum SSA	752:764	a maximum SSA of 246.48 m2 g-1	752:781	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	6	11	with	adsorbent	1206:1214	arg1	performance					1226:1236	high performance	1221:1236	high performance	1221:1236	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	0	12	from	application	151:161	arg1	bioseparation					166:178	bioseparation	166:178	bioseparation	166:178	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	3	13	theme	agarose	531:537	arg1	chains					539:544	the agarose chains	527:544	the agarose chains in the chitosan/agarose composite microspheres (CAM)	527:597	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	6	14	theme	high	1221:1224	arg1	performance					1226:1236	high performance	1221:1236	high performance	1221:1236	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	2	15	dep	strategy	356:363	arg1	develop					368:374	develop	368:374	to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent	365:488	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	5	16	dep	rate	997:1000	arg1	reaching					1003:1010	reaching	1003:1010	reaching equilibrium in less than 15 min	1003:1042	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	4	17	theme	hierarchical	718:729	arg1	structure					738:746	hierarchical porous structure	718:746	hierarchical porous structure	718:746	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	2	18	theme	high	417:420	arg1	SSA					445:447	SSA	445:447	SSA	445:447	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	18	theme	high	417:420	arg1	area					439:442	high specific surface area	417:442	high specific surface area (SSA)	417:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	5	19	theme	high	939:942	arg1	capacity					955:962	a high adsorption capacity	937:962	a high adsorption capacity (301.88 mg g-1)	937:978	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	19	theme	high	939:942	arg1	g-1					975:977	301.88 mg g-1	965:977	301.88 mg g-1	965:977	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	20	theme	adsorbent	825:833	arg1	protein					817:823	a protein adsorbent	815:833	a protein adsorbent	815:833	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	21	dep	affinity	884:891	arg1	denoted					904:910	denoted	904:910	denoted as Cu2+PCM-15	904:924	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	22	theme	adsorption	944:953	arg1	capacity					955:962	a high adsorption capacity	937:962	a high adsorption capacity (301.88 mg g-1)	937:978	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	22	theme	adsorption	944:953	arg1	g-1					975:977	301.88 mg g-1	965:977	301.88 mg g-1	965:977	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	6	23	contain	has	1238:1240	arg1	affinity					1197:1204	the as-prepared affinity	1181:1204	the as-prepared affinity adsorbent with high performance	1181:1236	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	6	23	contain	has	1238:1240	arg2	potential					1260:1268	great application potential	1242:1268	great application potential	1242:1268	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	4	24	theme	porous	731:736	arg1	structure					738:746	hierarchical porous structure	718:746	hierarchical porous structure	718:746	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	6	25	theme	as-prepared	1185:1195	arg1	affinity					1197:1204	the as-prepared affinity	1181:1204	the as-prepared affinity adsorbent with high performance	1181:1236	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	3	26	theme	chitosan/agarose	553:568	arg1	CAM					594:596	CAM	594:596	CAM	594:596	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	26	theme	chitosan/agarose	553:568	arg1	microspheres					580:591	the chitosan/agarose composite microspheres	549:591	the chitosan/agarose composite microspheres (CAM)	549:597	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	2	27	theme	chitosan	383:390	arg1	PCMs					406:409	PCMs	406:409	PCMs	406:409	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	27	theme	chitosan	383:390	arg1	microspheres					392:403	porous chitosan microspheres	376:403	porous chitosan microspheres (PCMs) with high specific surface area (SSA)	376:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	0	28	theme	porous	20:25	arg1	microspheres					36:47	porous chitosan microspheres	20:47	porous chitosan microspheres with high specific surface area	20:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	1	29	theme	protein	298:304	arg1	separation					306:315	protein separation	298:315	protein separation	298:315	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	1	30	theme	high	211:214	arg1	performance					235:245	synchronously high protein adsorption performance	197:245	synchronously high protein adsorption performance	197:245	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	2	31	theme	surface	431:437	arg1	SSA					445:447	SSA	445:447	SSA	445:447	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	31	theme	surface	431:437	arg1	area					439:442	high specific surface area	417:442	high specific surface area (SSA)	417:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	0	32	theme	pore-forming	105:116	arg1	agarose					90:96	agarose	90:96	agarose	90:96	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	0	32	theme	pore-forming	105:116	arg1	agent					118:122	the pore-forming agent	101:122	the pore-forming agent	101:122	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	1	33	theme	protein	216:222	arg1	performance					235:245	synchronously high protein adsorption performance	197:245	synchronously high protein adsorption performance	197:245	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	0	34	theme	microspheres	36:47	arg1	construction					4:15	The construction	0:15	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.	0:179	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	1	35	theme	adsorption	224:233	arg1	performance					235:245	synchronously high protein adsorption performance	197:245	synchronously high protein adsorption performance	197:245	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	0	36	theme	chitosan	27:34	arg1	microspheres					36:47	porous chitosan microspheres	20:47	porous chitosan microspheres with high specific surface area	20:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	6	37	theme	application	1248:1258	arg1	potential					1260:1268	great application potential	1242:1268	great application potential	1242:1268	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	0	38	theme	high	54:57	arg1	area					76:79	high specific surface area	54:79	high specific surface area	54:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	3	39	theme	composite	570:578	arg1	CAM					594:596	CAM	594:596	CAM	594:596	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	39	theme	composite	570:578	arg1	microspheres					580:591	the chitosan/agarose composite microspheres	549:591	the chitosan/agarose composite microspheres (CAM)	549:597	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	2	40	theme	effective	346:354	arg1	strategy					356:363	a facile yet effective strategy	333:363	a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent	333:488	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	6	41	theme	environmental-friendliness	1110:1135	arg1	chitosan					1159:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	0	42	theme	functionalized	136:149	arg1	application					151:161	functionalized application	136:161	functionalized application in bioseparation	136:178	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	5	43	theme	adsorption	986:995	arg1	rate					997:1000	fast adsorption rate	981:1000	fast adsorption rate (reaching equilibrium in less than 15 min)	981:1043	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	44	theme	fast	981:984	arg1	rate					997:1000	fast adsorption rate	981:1000	fast adsorption rate (reaching equilibrium in less than 15 min)	981:1043	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	3	45	theme	SSA	688:690	arg1	improvement					673:683	the improvement	669:683	the improvement of SSA	669:690	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	45	theme	SSA	688:690	arg1	generation					628:637	the generation	624:637	the generation of nanopores/nanochannels	624:663	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	46	from	chains	539:544	arg1	CAM					594:596	CAM	594:596	CAM	594:596	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	46	from	chains	539:544	arg1	microspheres					580:591	the chitosan/agarose composite microspheres	549:591	the chitosan/agarose composite microspheres (CAM)	549:597	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	5	47	theme	mg	972:973	arg1	capacity					955:962	a high adsorption capacity	937:962	a high adsorption capacity (301.88 mg g-1)	937:978	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	5	47	theme	mg	972:973	arg1	g-1					975:977	301.88 mg g-1	965:977	301.88 mg g-1	965:977	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	4	48	theme	obtained	697:704	arg1	PCMs					706:709	The obtained PCMs	693:709	The obtained PCMs	693:709	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	4	49	theme	m2	776:777	arg1	g-1					779:781	246.48 m2 g-1	769:781	246.48 m2 g-1	769:781	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	2	50	theme	facile	335:340	arg1	strategy					356:363	a facile yet effective strategy	333:363	a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent	333:488	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	1	51	with	Adsorbents	181:190	arg1	route					270:274	a facile synthetic route	251:274	a facile synthetic route	251:274	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	1	51	with	Adsorbents	181:190	arg1	performance					235:245	synchronously high protein adsorption performance	197:245	synchronously high protein adsorption performance	197:245	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	5	52	theme	Cu2+	879:882	arg1	affinity					884:891	the resultant immobilized Cu2+ affinity adsorbent	853:901	the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15)	853:925	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	4	53	theme	246.48	769:774	arg1	g-1					779:781	246.48 m2 g-1	769:781	246.48 m2 g-1	769:781	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	5	54	theme	adsorbent	893:901	arg1	affinity					884:891	the resultant immobilized Cu2+ affinity adsorbent	853:901	the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15)	853:925	For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg g-1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb.
31429461	0	55	theme	surface	68:74	arg1	area					76:79	high specific surface area	54:79	high specific surface area	54:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	3	56	theme	nanopores/nanochannels	642:663	arg1	improvement					673:683	the improvement	669:683	the improvement of SSA	669:690	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	3	56	theme	nanopores/nanochannels	642:663	arg1	generation					628:637	the generation	624:637	the generation of nanopores/nanochannels	624:663	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	6	57	theme	abundant	1142:1149	arg1	chitosan					1159:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	its environmental-friendliness, and abundant biomass chitosan	1106:1166	Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.
31429461	2	58	theme	specific	422:429	arg1	SSA					445:447	SSA	445:447	SSA	445:447	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	58	theme	specific	422:429	arg1	area					439:442	high specific surface area	417:442	high specific surface area (SSA)	417:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	59	theme	porous	376:381	arg1	PCMs					406:409	PCMs	406:409	PCMs	406:409	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	59	theme	porous	376:381	arg1	microspheres					392:403	porous chitosan microspheres	376:403	porous chitosan microspheres (PCMs) with high specific surface area (SSA)	376:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	1	60	theme	facile	253:258	arg1	route					270:274	a facile synthetic route	251:274	a facile synthetic route	251:274	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31429461	0	61	theme	specific	59:66	arg1	area					76:79	high specific surface area	54:79	high specific surface area	54:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	2	62	with	microspheres	392:403	arg1	SSA					445:447	SSA	445:447	SSA	445:447	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	2	62	with	microspheres	392:403	arg1	area					439:442	high specific surface area	417:442	high specific surface area (SSA)	417:448	In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported.
31429461	4	63	theme	g-1	779:781	arg1	structure					738:746	hierarchical porous structure	718:746	hierarchical porous structure	718:746	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	4	63	theme	g-1	779:781	arg1	SSA					762:764	a maximum SSA	752:764	a maximum SSA of 246.48 m2 g-1	752:781	The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 g-1.
31429461	0	64	with	microspheres	36:47	arg1	area					76:79	high specific surface area	54:79	high specific surface area	54:79	The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation.
31429461	3	65	theme	heat	511:514	arg1	treatment					516:524	heat treatment	511:524	heat treatment	511:524	Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA.
31429461	1	66	theme	synthetic	260:268	arg1	route					270:274	a facile synthetic route	251:274	a facile synthetic route	251:274	Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation.
31030072	0	0	theme	binary	89:94	arg1	systems					96:102	single and binary systems	78:102	single and binary systems	78:102	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	1	1	theme	ACeMt	357:361	arg1	area					349:352	the porosity and specific surface area	315:352	the porosity and specific surface area of ACeMt	315:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	7	2	from	efficient	1066:1074	arg1	removal					1083:1089	the removal	1079:1089	the removal of dyes for water clarification	1079:1121	Overall, ACeMt is a promising bioadsorbent and highly efficient in the removal of dyes for water clarification.
31030072	5	3	dep	higher	722:727	arg1	55 °C					837:841	55 °C	837:841	55 °C	837:841	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	7	4	theme	dyes	1094:1097	arg1	removal					1083:1089	the removal	1079:1089	the removal of dyes for water clarification	1079:1121	Overall, ACeMt is a promising bioadsorbent and highly efficient in the removal of dyes for water clarification.
31030072	2	5	theme	resulting	368:376	arg1	bioadsorbent					378:389	The resulting bioadsorbent	364:389	The resulting bioadsorbent	364:389	The resulting bioadsorbent was characterized by means of SEM, FTIR and BET analysis and used for dye removal.
31030072	6	6	theme	Redlich-Peterson	904:919	arg1	model					921:925	Redlich-Peterson model	904:925	Redlich-Peterson model	904:925	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	1	7	theme	pore-forming	284:295	arg1	carbonate					269:277	ultra-fine calcium carbonate	250:277	ultra-fine calcium carbonate	250:277	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	7	theme	pore-forming	284:295	arg1	agent					297:301	a pore-forming agent	282:301	a pore-forming agent to increase the porosity and specific surface area of ACeMt	282:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	5	8	theme	conventional	743:754	arg1	adsorbents					756:765	conventional adsorbents	743:765	conventional adsorbents	743:765	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	0	9	from	removal	67:73	arg1	systems					96:102	single and binary systems	78:102	single and binary systems	78:102	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	7	10	from	removal	1083:1089	arg1	efficient					1066:1074	efficient	1066:1074	efficient	1066:1074	Overall, ACeMt is a promising bioadsorbent and highly efficient in the removal of dyes for water clarification.
31030072	5	11	theme	dye	811:813	arg1	removal					789:795	the removal	785:795	the removal of Auramine O dye	785:813	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	5	12	theme	adsorption	644:653	arg1	higher					722:727	higher	722:727	higher	722:727	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	5	12	theme	adsorption	644:653	arg1	capacities					655:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	5	13	theme	maximum	636:642	arg1	higher					722:727	higher	722:727	higher	722:727	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	5	13	theme	maximum	636:642	arg1	capacities					655:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	3	14	theme	kinetic	541:547	arg1	models					549:554	kinetic models	541:554	kinetic models	541:554	Adsorption experimental data were fitted with various isotherm and kinetic models.
31030072	0	15	theme	dye	63:65	arg1	removal					67:73	dye removal	63:73	dye removal in single and binary systems	63:102	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	2	16	used	used	452:455	arg2	bioadsorbent					378:389	The resulting bioadsorbent	364:389	The resulting bioadsorbent	364:389	The resulting bioadsorbent was characterized by means of SEM, FTIR and BET analysis and used for dye removal.
31030072	7	17	theme	water	1103:1107	arg1	clarification					1109:1121	water clarification	1103:1121	water clarification	1103:1121	Overall, ACeMt is a promising bioadsorbent and highly efficient in the removal of dyes for water clarification.
31030072	6	18	theme	isotherm	954:961	arg1	model					963:967	uncompetitive Langmuir isotherm model	931:967	uncompetitive Langmuir isotherm model	931:967	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	6	19	theme	single	972:977	arg1	system					990:995	single and binary system	972:995	system	990:995	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	6	20	from	model	921:925	arg1	system					990:995	single and binary system	972:995	system	990:995	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	5	21	theme	ACeMt	669:673	arg1	higher					722:727	higher	722:727	higher	722:727	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	5	21	theme	ACeMt	669:673	arg1	capacities					655:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities	632:664	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	0	22	theme	composite	43:51	arg1	beads					53:57	mesoporous composite beads	32:57	mesoporous composite beads	32:57	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	6	23	theme	binary	983:988	arg1	system					990:995	single and binary system	972:995	system	990:995	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	1	24	theme	Fine	105:108	arg1	ACeMt					173:177	ACeMt	173:177	ACeMt	173:177	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	24	theme	Fine	105:108	arg1	beads					166:170	Fine aminated cellulose/montmorillonite mesoporous composite beads	105:170	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt)	105:178	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	24	theme	Fine	105:108	arg1	adsorbent					209:217	adsorbent	209:217	adsorbent	209:217	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	2	25	theme	BET	435:437	arg1	analysis					439:446	BET analysis	435:446	BET analysis	435:446	The resulting bioadsorbent was characterized by means of SEM, FTIR and BET analysis and used for dye removal.
31030072	0	26	theme	mesoporous	32:41	arg1	beads					53:57	mesoporous composite beads	32:57	mesoporous composite beads	32:57	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	4	27	theme	chemical	584:591	arg1	adsorption					593:602	chemical adsorption	584:602	chemical adsorption	584:602	The results indicated that chemical adsorption was the rate-limiting step.
31030072	4	27	theme	chemical	584:591	arg1	step					626:629	the rate-limiting step	608:629	the rate-limiting step	608:629	The results indicated that chemical adsorption was the rate-limiting step.
31030072	1	28	theme	aminated	110:117	arg1	ACeMt					173:177	ACeMt	173:177	ACeMt	173:177	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	28	theme	aminated	110:117	arg1	beads					166:170	Fine aminated cellulose/montmorillonite mesoporous composite beads	105:170	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt)	105:178	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	28	theme	aminated	110:117	arg1	adsorbent					209:217	adsorbent	209:217	adsorbent	209:217	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	3	29	theme	Adsorption	474:483	arg1	data					498:501	Adsorption experimental data	474:501	Adsorption experimental data	474:501	Adsorption experimental data were fitted with various isotherm and kinetic models.
31030072	1	30	theme	porosity	319:326	arg1	area					349:352	the porosity and specific surface area	315:352	the porosity and specific surface area of ACeMt	315:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	31	theme	novel	225:229	arg1	approach					231:238	a novel approach	223:238	a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt	223:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	5	32	theme	Auramine	800:807	arg1	dye					811:813	Auramine O dye	800:813	Auramine O dye	800:813	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	6	33	from	equilibrium	864:874	arg1	adsorptions					849:859	The adsorptions	845:859	The adsorptions at equilibrium	845:874	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	5	34	theme	O	809:809	arg1	dye					811:813	Auramine O dye	800:813	Auramine O dye	800:813	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	1	35	theme	specific	332:339	arg1	area					349:352	the porosity and specific surface area	315:352	the porosity and specific surface area of ACeMt	315:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	5	36	theme	Langmuir	696:703	arg1	model					705:709	Langmuir model	696:709	Langmuir model	696:709	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
31030072	1	37	theme	cellulose/montmorillonite	119:143	arg1	ACeMt					173:177	ACeMt	173:177	ACeMt	173:177	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	37	theme	cellulose/montmorillonite	119:143	arg1	beads					166:170	Fine aminated cellulose/montmorillonite mesoporous composite beads	105:170	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt)	105:178	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	37	theme	cellulose/montmorillonite	119:143	arg1	adsorbent					209:217	adsorbent	209:217	adsorbent	209:217	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	2	38	theme	dye	461:463	arg1	removal					465:471	dye removal	461:471	dye removal	461:471	The resulting bioadsorbent was characterized by means of SEM, FTIR and BET analysis and used for dye removal.
31030072	1	39	theme	surface	341:347	arg1	area					349:352	the porosity and specific surface area	315:352	the porosity and specific surface area of ACeMt	315:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	3	40	theme	experimental	485:496	arg1	data					498:501	Adsorption experimental data	474:501	Adsorption experimental data	474:501	Adsorption experimental data were fitted with various isotherm and kinetic models.
31030072	3	41	theme	various	520:526	arg1	isotherm					528:535	various isotherm	520:535	various isotherm	520:535	Adsorption experimental data were fitted with various isotherm and kinetic models.
31030072	1	42	theme	mesoporous	145:154	arg1	ACeMt					173:177	ACeMt	173:177	ACeMt	173:177	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	42	theme	mesoporous	145:154	arg1	beads					166:170	Fine aminated cellulose/montmorillonite mesoporous composite beads	105:170	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt)	105:178	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	42	theme	mesoporous	145:154	arg1	adsorbent					209:217	adsorbent	209:217	adsorbent	209:217	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	6	43	theme	Langmuir	945:952	arg1	model					963:967	uncompetitive Langmuir isotherm model	931:967	uncompetitive Langmuir isotherm model	931:967	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	1	44	theme	composite	156:164	arg1	ACeMt					173:177	ACeMt	173:177	ACeMt	173:177	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	44	theme	composite	156:164	arg1	beads					166:170	Fine aminated cellulose/montmorillonite mesoporous composite beads	105:170	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt)	105:178	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	44	theme	composite	156:164	arg1	adsorbent					209:217	adsorbent	209:217	adsorbent	209:217	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	6	45	from	model	963:967	arg1	system					990:995	single and binary system	972:995	system	990:995	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	1	46	theme	ultra-fine	250:259	arg1	carbonate					269:277	ultra-fine calcium carbonate	250:277	ultra-fine calcium carbonate	250:277	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	46	theme	ultra-fine	250:259	arg1	agent					297:301	a pore-forming agent	282:301	a pore-forming agent to increase the porosity and specific surface area of ACeMt	282:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	0	47	theme	single	78:83	arg1	systems					96:102	single and binary systems	78:102	single and binary systems	78:102	Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.
31030072	1	48	theme	calcium	261:267	arg1	carbonate					269:277	ultra-fine calcium carbonate	250:277	ultra-fine calcium carbonate	250:277	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	1	48	theme	calcium	261:267	arg1	agent					297:301	a pore-forming agent	282:301	a pore-forming agent to increase the porosity and specific surface area of ACeMt	282:361	Fine aminated cellulose/montmorillonite mesoporous composite beads (ACeMt) were prepared as green-based adsorbent via a novel approach utilizing ultra-fine calcium carbonate as a pore-forming agent to increase the porosity and specific surface area of ACeMt.
31030072	4	49	theme	rate-limiting	612:624	arg1	adsorption					593:602	chemical adsorption	584:602	chemical adsorption	584:602	The results indicated that chemical adsorption was the rate-limiting step.
31030072	4	49	theme	rate-limiting	612:624	arg1	step					626:629	the rate-limiting step	608:629	the rate-limiting step	608:629	The results indicated that chemical adsorption was the rate-limiting step.
31030072	6	50	theme	uncompetitive	931:943	arg1	model					963:967	uncompetitive Langmuir isotherm model	931:967	uncompetitive Langmuir isotherm model	931:967	The adsorptions at equilibrium were better correlated with Redlich-Peterson model and uncompetitive Langmuir isotherm model in single and binary system, respectively.
31030072	5	51	dep	55 °C	837:841	arg1	to					819:820	to	819:820	to	819:820	The maximum adsorption capacities of ACeMt, better described by Langmuir model, were much higher than those of conventional adsorbents, particularly for the removal of Auramine O dye (up to 1336.2 mg/g at 55 °C).
30658820	0	0	theme	value-added	82:92	arg1	utilization					94:104	high value-added utilization	77:104	high value-added utilization of clay and biology resources	77:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	5	1	theme	scanning	1119:1126	arg1	microscope					1137:1146	scanning electron microscope	1119:1146	scanning electron microscope (SEM)	1119:1152	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	1	theme	scanning	1119:1126	arg1	SEM					1149:1151	SEM	1149:1151	SEM	1149:1151	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	0	2	theme	high	77:80	arg1	utilization					94:104	high value-added utilization	77:104	high value-added utilization of clay and biology resources	77:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	1	3	theme	bacterial	173:181	arg1	BC					194:195	BC	194:195	BC	194:195	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	1	3	theme	bacterial	173:181	arg1	cellulose					183:191	bacterial cellulose	173:191	bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG)	173:263	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	6	4	theme	low-cost	1384:1391	arg1	resources					1405:1413	low-cost and natural resources	1384:1413	low-cost and natural resources	1384:1413	Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources.
30658820	5	5	theme	electron	1128:1135	arg1	microscope					1137:1146	scanning electron microscope	1119:1146	scanning electron microscope (SEM)	1119:1152	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	5	theme	electron	1128:1135	arg1	SEM					1149:1151	SEM	1149:1151	SEM	1149:1151	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	4	6	theme	water	887:891	arg1	retention					893:901	water retention	887:901	water retention	887:901	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	5	7	theme	sodium-based	937:948	arg1	bentonite					950:958	sodium-based bentonite	937:958	sodium-based bentonite	937:958	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	8	theme	composite	969:977	arg1	structure					979:987	composite structure	969:987	composite structure	969:987	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	2	9	from	effect	291:296	arg1	viscosity					423:431	the viscosity	419:431	the viscosity of BIG	419:438	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	2	10	theme	synthesis	469:477	arg1	process					479:485	the synthesis process	465:485	the synthesis process	465:485	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	3	11	theme	monomer	659:665	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	0	12	dep	clay	109:112	arg1	resources					126:134	resources	126:134	resources	126:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	2	13	theme	BIG	270:272	arg1	preparation					274:284	BIG preparation	270:284	BIG preparation	270:284	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	3	14	theme	neutralization	667:680	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	5	15	theme	original	917:924	arg1	bentonite					926:934	The original bentonite	913:934	The original bentonite	913:934	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	16	theme	water	1253:1257	arg1	absorption					1259:1268	water absorption	1253:1268	water absorption	1253:1268	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	2	17	theme	sodium-modification	358:376	arg1	content					325:331	content	325:331	content	325:331	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	2	17	theme	sodium-modification	358:376	arg1	time					350:353	time	350:353	time	350:353	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	2	17	theme	sodium-modification	358:376	arg1	temperature					334:344	temperature	334:344	temperature	334:344	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	0	18	theme	clay	109:112	arg1	utilization					94:104	high value-added utilization	77:104	high value-added utilization of clay and biology resources	77:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	3	19	theme	crosslinker	646:656	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	19	theme	crosslinker	646:656	arg1	BC					590:591	modified BC	581:591	modified BC from in situ fermentation	581:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	19	theme	crosslinker	646:656	arg1	time					715:718	time	715:718	time	715:718	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	19	theme	crosslinker	646:656	arg1	content					621:627	content	621:627	content of initiator and crosslinker	621:656	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	19	theme	crosslinker	646:656	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	19	theme	crosslinker	646:656	arg1	temperature					699:709	reaction temperature	690:709	reaction temperature	690:709	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	6	20	theme	superabsorbent	1354:1367	arg1	synthesis					1369:1377	superabsorbent synthesis	1354:1377	superabsorbent synthesis from low-cost and natural resources	1354:1413	Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources.
30658820	5	21	theme	absorption	1259:1268	arg1	performance					1238:1248	the performance	1234:1248	the performance of water absorption and thermal stability of the composite	1234:1307	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	2	22	theme	BIG	436:438	arg1	viscosity					423:431	the viscosity	419:431	the viscosity of BIG	419:438	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	4	23	theme	optimal	802:808	arg1	condition					810:818	optimal condition	802:818	optimal condition	802:818	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	5	24	theme	nuclear	1036:1042	arg1	NMR					1064:1066	NMR	1064:1066	NMR	1064:1066	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	24	theme	nuclear	1036:1042	arg1	resonance					1053:1061	nuclear magnetic resonance	1036:1061	nuclear magnetic resonance (NMR)	1036:1067	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	24	theme	nuclear	1036:1042	arg1	fluorescence					1016:1027	X-ray fluorescence	1010:1027	X-ray fluorescence (XRF)	1010:1033	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	0	25	theme	Bacterial	0:8	arg1	cellulose					10:18	Bacterial cellulose	0:18	Bacterial cellulose	0:18	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	3	26	theme	reaction	690:697	arg1	temperature					699:709	reaction temperature	690:709	reaction temperature	690:709	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	time					715:718	time	715:718	time	715:718	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	content					621:627	content	621:627	content of initiator and crosslinker	621:656	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	factors					532:538	different factors	522:538	different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time	522:718	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	27	theme	different	522:530	arg1	temperature					699:709	reaction temperature	690:709	reaction temperature	690:709	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	0	28	theme	superabsorbent	26:39	arg1	production					41:50	superabsorbent production	26:50	superabsorbent production	26:50	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	4	29	theme	salts	865:869	arg1	absorption					871:880	salts absorption	865:880	salts absorption	865:880	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	0	30	theme	biology	118:124	arg1	utilization					94:104	high value-added utilization	77:104	high value-added utilization of clay and biology resources	77:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	5	31	theme	magnetic	1044:1051	arg1	NMR					1064:1066	NMR	1064:1066	NMR	1064:1066	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	31	theme	magnetic	1044:1051	arg1	resonance					1053:1061	nuclear magnetic resonance	1036:1061	nuclear magnetic resonance (NMR)	1036:1067	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	31	theme	magnetic	1044:1051	arg1	fluorescence					1016:1027	X-ray fluorescence	1010:1027	X-ray fluorescence (XRF)	1010:1033	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	2	32	theme	gelling	383:389	arg1	type					399:402	gelling agent's type	383:402	gelling agent's type	383:402	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	3	33	theme	in	598:599	arg1	fermentation					606:617	in situ fermentation	598:617	in situ fermentation	598:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	1	34	theme	in	211:212	arg1	fermentation					219:230	in situ fermentation	211:230	in situ fermentation on bentonite inorganic gel (BIG)	211:263	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	4	35	theme	absorption	871:880	arg1	capacity					903:910	good water absorption, salts absorption, and water retention capacity	842:910	good water absorption, salts absorption, and water retention capacity	842:910	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	3	36	theme	modified	581:588	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	36	theme	modified	581:588	arg1	BC					590:591	modified BC	581:591	modified BC from in situ fermentation	581:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	6	37	from	resources	1405:1413	arg1	synthesis					1369:1377	superabsorbent synthesis	1354:1377	superabsorbent synthesis from low-cost and natural resources	1354:1413	Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources.
30658820	5	38	theme	composite	1299:1307	arg1	absorption					1259:1268	water absorption	1253:1268	water absorption	1253:1268	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	38	theme	composite	1299:1307	arg1	stability					1282:1290	thermal stability	1274:1290	thermal stability	1274:1290	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	4	39	theme	retention	893:901	arg1	capacity					903:910	good water absorption, salts absorption, and water retention capacity	842:910	good water absorption, salts absorption, and water retention capacity	842:910	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	0	40	theme	promising	55:63	arg1	example					65:71	A promising example	53:71	A promising example for high value-added utilization of clay and biology resources	53:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	4	41	theme	good	842:845	arg1	capacity					903:910	good water absorption, salts absorption, and water retention capacity	842:910	good water absorption, salts absorption, and water retention capacity	842:910	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	3	42	theme	factors	532:538	arg1	effect					512:517	the effect	508:517	the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized	508:779	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	4	43	theme	absorption	853:862	arg1	capacity					903:910	good water absorption, salts absorption, and water retention capacity	842:910	good water absorption, salts absorption, and water retention capacity	842:910	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	5	44	theme	infrared	1088:1095	arg1	FT-IR					1111:1115	FT-IR	1111:1115	FT-IR	1111:1115	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	44	theme	infrared	1088:1095	arg1	spectroscopy					1097:1108	infrared spectroscopy	1088:1108	infrared spectroscopy (FT-IR)	1088:1116	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	1	45	from	fermentation	219:230	arg1	BIG					260:262	BIG	260:262	BIG	260:262	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	1	45	from	fermentation	219:230	arg1	gel					255:257	bentonite inorganic gel	235:257	bentonite inorganic gel (BIG)	235:263	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	1	46	dep	in	211:212	arg1	situ					214:217	situ	214:217	situ	214:217	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	3	47	from	effect	512:517	arg1	performance					727:737	the performance	723:737	the performance of composite (superabsorbent) synthesized	723:779	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	48	theme	composite	742:750	arg1	performance					727:737	the performance	723:737	the performance of composite (superabsorbent) synthesized	723:779	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	4	49	theme	water	847:851	arg1	absorption					853:862	water absorption	847:862	water absorption	847:862	Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity.
30658820	0	50	dep	based	20:24	arg1	example					65:71	A promising example	53:71	A promising example for high value-added utilization of clay and biology resources	53:134	Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
30658820	5	51	theme	thermal	1274:1280	arg1	stability					1282:1290	thermal stability	1274:1290	thermal stability	1274:1290	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	2	52	theme	sodium	301:306	arg1	type					316:319	sodium agent's type	301:319	sodium agent's type	301:319	For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process.
30658820	5	53	theme	X-ray	1010:1014	arg1	resonance					1053:1061	nuclear magnetic resonance	1036:1061	nuclear magnetic resonance (NMR)	1036:1067	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	53	theme	X-ray	1010:1014	arg1	XRF					1030:1032	XRF	1030:1032	XRF	1030:1032	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	53	theme	X-ray	1010:1014	arg1	fluorescence					1016:1027	X-ray fluorescence	1010:1027	X-ray fluorescence (XRF)	1010:1033	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	3	54	theme	monomer	559:565	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	54	theme	monomer	559:565	arg1	BC					590:591	modified BC	581:591	modified BC from in situ fermentation	581:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	54	theme	monomer	559:565	arg1	time					715:718	time	715:718	time	715:718	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	54	theme	monomer	559:565	arg1	content					621:627	content	621:627	content of initiator and crosslinker	621:656	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	54	theme	monomer	559:565	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	54	theme	monomer	559:565	arg1	temperature					699:709	reaction temperature	690:709	reaction temperature	690:709	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	5	55	theme	stability	1282:1290	arg1	performance					1238:1248	the performance	1234:1248	the performance of water absorption and thermal stability of the composite	1234:1307	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	56	theme	thermogravimetric	1158:1174	arg1	TGA					1186:1188	TGA	1186:1188	TGA	1186:1188	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	5	56	theme	thermogravimetric	1158:1174	arg1	analysis					1176:1183	thermogravimetric analysis	1158:1183	thermogravimetric analysis (TGA)	1158:1189	The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite.
30658820	1	57	theme	bentonite	235:243	arg1	BIG					260:262	BIG	260:262	BIG	260:262	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	1	57	theme	bentonite	235:243	arg1	gel					255:257	bentonite inorganic gel	235:257	bentonite inorganic gel (BIG)	235:263	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	3	58	from	fermentation	606:617	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	58	from	fermentation	606:617	arg1	BC					590:591	modified BC	581:591	modified BC from in situ fermentation	581:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	ratio					550:554	ratio	550:554	ratio of monomer to substrate (modified BC from in situ fermentation)	550:618	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	BC					590:591	modified BC	581:591	modified BC from in situ fermentation	581:617	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	time					715:718	time	715:718	time	715:718	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	content					621:627	content	621:627	content of initiator and crosslinker	621:656	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	degree					682:687	monomer neutralization degree	659:687	monomer neutralization degree	659:687	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	59	theme	initiator	632:640	arg1	temperature					699:709	reaction temperature	690:709	reaction temperature	690:709	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	3	60	dep	in	598:599	arg1	situ					601:604	situ	601:604	situ	601:604	For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed.
30658820	6	61	theme	natural	1397:1403	arg1	resources					1405:1413	low-cost and natural resources	1384:1413	low-cost and natural resources	1384:1413	Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources.
30658820	1	62	theme	inorganic	245:253	arg1	BIG					260:262	BIG	260:262	BIG	260:262	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
30658820	1	62	theme	inorganic	245:253	arg1	gel					255:257	bentonite inorganic gel	235:257	bentonite inorganic gel (BIG)	235:263	Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG).
31145956	5	0	theme	Cu	796:797	arg1	rate					788:791	The removal rate	776:791	The removal rate of Cu(II) by Mag-Ben/CCS/Alg	776:820	The removal rate of Cu(II) by Mag-Ben/CCS/Alg still remained above 80% after recycling four times.
31145956	6	1	theme	adsorption	879:888	arg1	process					890:896	The adsorption process	875:896	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg	875:925	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
31145956	3	2	theme	adsorption	472:481	arg1	dosage					464:469	the adsorbent dosage	450:469	the adsorbent dosage	450:469	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	2	theme	adsorption	472:481	arg1	time					483:486	adsorption time	472:486	adsorption time	472:486	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	1	3	theme	copper	280:285	arg1	ions					287:290	copper ions	280:290	copper ions	280:290	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	6	4	theme	quasi-second-order	959:976	arg1	model					986:990	the quasi-second-order kinetic model	955:990	the quasi-second-order kinetic model	955:990	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
31145956	1	5	theme	composite	126:134	arg1	beads					145:149	composite hydrogel beads	126:149	composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg)	126:234	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	3	6	theme	adsorbent	454:462	arg1	temperature					493:503	temperature	493:503	temperature	493:503	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	6	theme	adsorbent	454:462	arg1	time					483:486	adsorption time	472:486	adsorption time	472:486	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	6	theme	adsorbent	454:462	arg1	pH					489:490	pH	489:490	pH	489:490	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	6	theme	adsorbent	454:462	arg1	dosage					464:469	the adsorbent dosage	450:469	the adsorbent dosage	450:469	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	6	theme	adsorbent	454:462	arg1	concentration					518:530	initial concentration	510:530	initial concentration	510:530	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	2	7	dep	SEM	383:385	arg1	tests					428:432	potential tests	418:432	potential tests	418:432	The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests.
31145956	1	8	theme	magnetic	160:167	arg1	Mag-Ben/CCS/Alg					219:233	Mag-Ben/CCS/Alg	219:233	Mag-Ben/CCS/Alg	219:233	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	1	8	theme	magnetic	160:167	arg1	alginate					209:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate	160:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg)	160:234	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	7	9	theme	adsorption	1030:1039	arg1	spontaneous					1053:1063	spontaneous	1053:1063	spontaneous	1053:1063	The adsorption process was spontaneous and endothermic.
31145956	7	9	theme	adsorption	1030:1039	arg1	process					1041:1047	The adsorption process	1026:1047	The adsorption process	1026:1047	The adsorption process was spontaneous and endothermic.
31145956	1	10	theme	bentonite/carboxymethyl	169:191	arg1	Mag-Ben/CCS/Alg					219:233	Mag-Ben/CCS/Alg	219:233	Mag-Ben/CCS/Alg	219:233	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	1	10	theme	bentonite/carboxymethyl	169:191	arg1	alginate					209:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate	160:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg)	160:234	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	2	11	theme	potential	418:426	arg1	tests					428:432	potential tests	418:432	potential tests	418:432	The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests.
31145956	1	12	theme	chitosan/sodium	193:207	arg1	Mag-Ben/CCS/Alg					219:233	Mag-Ben/CCS/Alg	219:233	Mag-Ben/CCS/Alg	219:233	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	1	12	theme	chitosan/sodium	193:207	arg1	alginate					209:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate	160:216	magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg)	160:234	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	4	13	theme	removal	674:680	arg1	rate					682:685	the removal rate	670:685	the removal rate of Cu(II)	670:695	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	4	13	theme	removal	674:680	arg1	%					713:713	92.62 ± 0.39%	701:713	92.62 ± 0.39%	701:713	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	5	14	theme	removal	780:786	arg1	rate					788:791	The removal rate	776:791	The removal rate of Cu(II) by Mag-Ben/CCS/Alg	776:820	The removal rate of Cu(II) by Mag-Ben/CCS/Alg still remained above 80% after recycling four times.
31145956	0	15	theme	bentonite/carboxymethyl	24:46	arg1	beads					82:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	4	16	theme	Cu	690:691	arg1	rate					682:685	the removal rate	670:685	the removal rate of Cu(II)	670:695	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	4	16	theme	Cu	690:691	arg1	%					713:713	92.62 ± 0.39%	701:713	92.62 ± 0.39%	701:713	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	0	17	theme	magnetic	15:22	arg1	beads					82:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	3	18	theme	initial	510:516	arg1	dosage					464:469	the adsorbent dosage	450:469	the adsorbent dosage	450:469	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	18	theme	initial	510:516	arg1	concentration					518:530	initial concentration	510:530	initial concentration	510:530	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	0	19	theme	alginate	64:71	arg1	beads					82:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	6	20	theme	adsorption	1005:1014	arg1	isotherm					1016:1023	Langmuir adsorption isotherm	996:1023	Langmuir adsorption isotherm	996:1023	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
31145956	4	21	theme	maximum	724:730	arg1	56.79 mg/g					756:765	56.79 mg/g	756:765	56.79 mg/g (30 °C)	756:773	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	4	21	theme	maximum	724:730	arg1	capacity					743:750	the maximum adsorption capacity	720:750	the maximum adsorption capacity	720:750	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	0	22	theme	chitosan/sodium	48:62	arg1	beads					82:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	0	23	theme	beads	82:86	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.	0:110	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	3	24	theme	Cu	535:536	arg1	temperature					493:503	temperature	493:503	temperature	493:503	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	24	theme	Cu	535:536	arg1	pH					489:490	pH	489:490	pH	489:490	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	24	theme	Cu	535:536	arg1	dosage					464:469	the adsorbent dosage	450:469	the adsorbent dosage	450:469	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	24	theme	Cu	535:536	arg1	concentration					518:530	initial concentration	510:530	initial concentration	510:530	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	2	25	theme	composite	350:358	arg1	properties					332:341	properties	332:341	properties	332:341	The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests.
31145956	2	25	theme	composite	350:358	arg1	structure					318:326	structure	318:326	structure	318:326	The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests.
31145956	0	26	theme	hydrogel	73:80	arg1	beads					82:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads	15:86	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	4	27	theme	adsorption	732:741	arg1	56.79 mg/g					756:765	56.79 mg/g	756:765	56.79 mg/g (30 °C)	756:773	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	4	27	theme	adsorption	732:741	arg1	capacity					743:750	the maximum adsorption capacity	720:750	the maximum adsorption capacity	720:750	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	3	28	theme	dosage	464:469	arg1	effects					439:445	The effects	435:445	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results	435:566	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	4	29	theme	adsorption	658:667	arg1	90 min					648:653	90 min	648:653	90 min of adsorption	648:667	The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0.39%, and the maximum adsorption capacity was 56.79 mg/g (30 °C).
31145956	3	30	theme	adsorption	549:558	arg1	results					560:566	the adsorption results	545:566	the adsorption results	545:566	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	3	31	from	effects	439:445	arg1	results					560:566	the adsorption results	545:566	the adsorption results	545:566	The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied.
31145956	6	32	theme	Langmuir	996:1003	arg1	adsorption					1005:1014	Langmuir adsorption	996:1014	Langmuir adsorption isotherm	996:1023	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
31145956	2	33	dep	structure	318:326	arg1	The					314:316	The	314:316	The	314:316	The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests.
31145956	6	34	theme	Cu	901:902	arg1	process					890:896	The adsorption process	875:896	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg	875:925	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
31145956	0	35	theme	Cu	92:93	arg1	adsorption					100:109	Cu (II) adsorption	92:109	Cu (II) adsorption	92:109	Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
31145956	1	36	theme	hydrogel	136:143	arg1	beads					145:149	composite hydrogel beads	126:149	composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg)	126:234	In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied.
31145956	6	37	theme	kinetic	978:984	arg1	model					986:990	the quasi-second-order kinetic model	955:990	the quasi-second-order kinetic model	955:990	The adsorption process of Cu(II) by Mag-Ben/CCS/Alg occurred in accordance with the quasi-second-order kinetic model and Langmuir adsorption isotherm.
30318239	4	0	theme	mechanical	650:659	arg1	properties					661:670	the thermal and mechanical properties	634:670	the thermal and mechanical properties of PLA/CF composites	634:691	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	5	1	theme	RH	1023:1024	arg1	humidity					1007:1014	the high humidity	998:1014	the high humidity of 70% RH	998:1024	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	3	2	theme	60 °C/70	463:470	arg1	%					471:471	%	471:471	%	471:471	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	1	3	theme	twin-screw	204:213	arg1	extruder					215:222	a twin-screw extruder	202:222	a twin-screw extruder	202:222	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	4	4	theme	storage	791:797	arg1	modulus					799:805	the storage modulus	787:805	the storage modulus with increasing elapsed time	787:834	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	5	theme	%	471:471	arg1	humidity					453:460	constant humidity	444:460	constant humidity (60 °C/70% RH)	444:475	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	3	5	theme	%	471:471	arg1	RH					473:474	60 °C/70% RH	463:474	60 °C/70% RH	463:474	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	0	6	theme	PLA/CF	69:74	arg1	durability					55:64	durability	55:64	durability of PLA/CF	55:74	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	0	7	from	Effect	0:5	arg1	durability					55:64	durability	55:64	durability of PLA/CF	55:74	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	6	8	theme	surface	1067:1073	arg1	agent					1085:1089	the surface treatment agent	1063:1089	the surface treatment agent	1063:1089	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	5	9	theme	mechanical	886:895	arg1	properties					897:906	the thermal and mechanical properties	870:906	the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy	870:971	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	3	10	theme	higher-order	486:497	arg1	changes					509:515	the higher-order structure changes	482:515	the higher-order structure changes	482:515	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	1	11	theme	injection	228:236	arg1	molding					238:244	injection molding	228:244	injection molding	228:244	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	3	12	theme	Accelerated	310:320	arg1	tests					334:338	Accelerated degradation tests	310:338	Accelerated degradation tests	310:338	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	5	13	theme	70	1019:1020	arg1	%					1021:1021	%	1021:1021	%	1021:1021	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	6	14	theme	important	1167:1175	arg1	role					1177:1180	an important role	1164:1180	an important role	1164:1180	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	3	15	theme	degradation	322:332	arg1	tests					334:338	Accelerated degradation tests	310:338	Accelerated degradation tests	310:338	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	5	16	theme	%	1021:1021	arg1	RH					1023:1024	70% RH	1019:1024	70% RH	1019:1024	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	6	17	theme	treatment	1075:1083	arg1	agent					1085:1089	the surface treatment agent	1063:1089	the surface treatment agent	1063:1089	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	4	18	dep	RH	741:742	arg1	%					739:739	%	739:739	%	739:739	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	1	19	theme	polylactic	115:124	arg1	PLA					132:134	PLA	132:134	PLA	132:134	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	1	19	theme	polylactic	115:124	arg1	acid					126:129	polylactic acid	115:129	polylactic acid (PLA) matrix reinforced with cellulose fibers (CF)	115:180	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	2	20	theme	epoxy-based	272:282	arg1	agents					302:307	epoxy-based surface treatment agents	272:307	epoxy-based surface treatment agents	272:307	The CFs were coated with epoxy-based surface treatment agents.
30318239	6	21	theme	PLA	1215:1217	arg1	matrix					1219:1224	the PLA matrix	1211:1224	the PLA matrix	1211:1224	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	4	22	dep	%	739:739	arg1	70					737:738	70	737:738	70	737:738	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	4	22	dep	%	739:739	arg1	60 °C					727:731	60 °C	727:731	60 °C	727:731	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	6	23	theme	interfacial	1109:1119	arg1	adhesion					1121:1128	interfacial adhesion	1109:1128	interfacial adhesion between CF and PLA	1109:1147	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	4	24	theme	PLA/CF	675:680	arg1	composites					682:691	PLA/CF composites	675:691	PLA/CF composites	675:691	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	25	theme	degradation	521:531	arg1	characteristics					533:547	degradation characteristics	521:547	degradation characteristics	521:547	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	1	26	theme	cellulose	160:168	arg1	CF					178:179	CF	178:179	CF	178:179	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	1	26	theme	cellulose	160:168	arg1	fibers					170:175	cellulose fibers	160:175	cellulose fibers (CF)	160:180	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	0	27	theme	treatment	18:26	arg1	Effect					0:5	Effect	0:5	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF	0:74	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	5	28	theme	composites	922:931	arg1	properties					897:906	the thermal and mechanical properties	870:906	the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy	870:971	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	3	29	theme	structure	499:507	arg1	changes					509:515	the higher-order structure changes	482:515	the higher-order structure changes	482:515	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	4	30	dep	degradation	607:617	arg1	test					619:622	test	619:622	test at 60 °C	619:631	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	31	theme	constant	419:426	arg1	temperature					428:438	constant temperature	419:438	constant temperature	419:438	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	0	32	theme	surface	10:16	arg1	treatment					18:26	surface treatment	10:26	surface treatment of cellulose fiber (CF)	10:50	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	5	33	theme	thermal	874:880	arg1	properties					897:906	the thermal and mechanical properties	870:906	the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy	870:971	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	4	34	theme	thermal	638:644	arg1	properties					661:670	the thermal and mechanical properties	634:670	the thermal and mechanical properties of PLA/CF composites	634:691	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	0	35	theme	cellulose	31:39	arg1	CF					48:49	CF	48:49	CF	48:49	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	0	35	theme	cellulose	31:39	arg1	fiber					41:45	cellulose fiber	31:45	cellulose fiber (CF)	31:50	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	4	36	theme	melting	749:755	arg1	point					757:761	the melting point	745:761	the melting point	745:761	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	37	theme	high	387:390	arg1	60 °C					406:410	60 °C	406:410	60 °C	406:410	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	3	37	theme	high	387:390	arg1	temperatures					392:403	high temperatures	387:403	high temperatures (60 °C)	387:411	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	5	38	theme	high	1002:1005	arg1	humidity					1007:1014	the high humidity	998:1014	the high humidity of 70% RH	998:1024	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	3	39	theme	molded	556:561	arg1	products					563:570	the molded products	552:570	the molded products	552:570	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	4	40	theme	accelerated	595:605	arg1	degradation					607:617	the accelerated degradation	591:617	the accelerated degradation test at 60 °C	591:631	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	41	theme	PLA/CF	366:371	arg1	composites					373:382	these PLA/CF composites	360:382	these PLA/CF composites	360:382	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	6	42	theme	matrix	1219:1224	arg1	degradation					1196:1206	degradation	1196:1206	degradation of the PLA matrix	1196:1224	These results strongly suggest that the surface treatment agent not only improves interfacial adhesion between CF and PLA but also plays an important role in inhibiting degradation of the PLA matrix.
30318239	0	43	theme	fiber	41:45	arg1	treatment					18:26	surface treatment	10:26	surface treatment of cellulose fiber (CF)	10:50	Effect of surface treatment of cellulose fiber (CF) on durability of PLA/CF bio-composites.
30318239	4	44	theme	elapsed	823:829	arg1	time					831:834	elapsed time	823:834	elapsed time	823:834	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	5	45	theme	low-molecular-weight	946:965	arg1	epoxy					967:971	low-molecular-weight epoxy	946:971	low-molecular-weight epoxy	946:971	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	2	46	theme	treatment	292:300	arg1	agents					302:307	epoxy-based surface treatment agents	272:307	epoxy-based surface treatment agents	272:307	The CFs were coated with epoxy-based surface treatment agents.
30318239	5	47	theme	PLA/CF	915:920	arg1	composites					922:931	the PLA/CF composites	911:931	the PLA/CF composites treated with low-molecular-weight epoxy	911:971	However, the thermal and mechanical properties of the PLA/CF composites treated with low-molecular-weight epoxy did not degrade, even at the high humidity of 70% RH.
30318239	2	48	theme	surface	284:290	arg1	agents					302:307	epoxy-based surface treatment agents	272:307	epoxy-based surface treatment agents	272:307	The CFs were coated with epoxy-based surface treatment agents.
30318239	4	49	theme	composites	682:691	arg1	properties					661:670	the thermal and mechanical properties	634:670	the thermal and mechanical properties of PLA/CF composites	634:691	In the accelerated degradation test at 60 °C, the thermal and mechanical properties of PLA/CF composites showed no degradation, whereas at 60 °C and 70% RH, the melting point decreased ca. 25 °C and the storage modulus with increasing elapsed time decreased more than 50%.
30318239	3	50	theme	products	563:570	arg1	changes					509:515	the higher-order structure changes	482:515	the higher-order structure changes	482:515	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	3	50	theme	products	563:570	arg1	characteristics					533:547	degradation characteristics	521:547	degradation characteristics	521:547	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	1	51	theme	acid	126:129	arg1	matrix					137:142	polylactic acid (PLA) matrix	115:142	polylactic acid (PLA) matrix reinforced with cellulose fibers (CF)	115:180	Bio-composites made of polylactic acid (PLA) matrix reinforced with cellulose fibers (CF) were prepared using a twin-screw extruder and injection molding.
30318239	3	52	theme	constant	444:451	arg1	humidity					453:460	constant humidity	444:460	constant humidity (60 °C/70% RH)	444:475	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
30318239	3	52	theme	constant	444:451	arg1	RH					473:474	60 °C/70% RH	463:474	60 °C/70% RH	463:474	Accelerated degradation tests were carried out on these PLA/CF composites at high temperatures (60 °C) or at constant temperature and constant humidity (60 °C/70% RH), and the higher-order structure changes and degradation characteristics of the molded products were evaluated.
31473375	0	0	theme	enzymatic	87:95	arg1	hydrolysis					97:106	enzymatic hydrolysis	87:106	enzymatic hydrolysis	87:106	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	5	1	theme	surface	995:1001	arg1	area					1003:1006	specific surface area	986:1006	specific surface area	986:1006	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	2	theme	correlation	636:646	arg1	analyses					648:655	qualitative and quantitative correlation analyses	607:655	qualitative and quantitative correlation analyses of the parameters	607:673	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	5	3	theme	pore	1009:1012	arg1	volume					1014:1019	pore volume	1009:1019	pore volume	1009:1019	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	2	4	dep	pretreatment	297:308	arg1	pretreatment					365:376	dilute acid pretreatment	353:376	dilute acid pretreatment	353:376	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	2	4	dep	pretreatment	297:308	arg1	pretreatment					407:418	ammonia fiber expansion pretreatment	383:418	ammonia fiber expansion pretreatment	383:418	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	2	4	dep	pretreatment	297:308	arg1	pretreatment					339:350	alkaline hydroxide peroxide pretreatment	311:350	alkaline hydroxide peroxide pretreatment	311:350	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	5	5	contain	had	1083:1085	arg1	volume					1014:1019	pore volume	1009:1019	pore volume	1009:1019	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	5	contain	had	1083:1085	arg1	area					1065:1068	surface area	1057:1068	surface area of cellulose	1057:1081	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	5	contain	had	1083:1085	arg1	area					1003:1006	specific surface area	986:1006	specific surface area	986:1006	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	5	contain	had	1083:1085	arg1	volume					1045:1050	enzyme-accessible pore volume	1022:1050	enzyme-accessible pore volume	1022:1050	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	5	contain	had	1083:1085	arg2	correlations					1108:1119	significant positive correlations	1087:1119	significant positive correlations	1087:1119	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	5	contain	had	1083:1085	arg1	content					977:983	cellulose content	967:983	cellulose content	967:983	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	6	theme	quantitative	623:634	arg1	analyses					648:655	qualitative and quantitative correlation analyses	607:655	qualitative and quantitative correlation analyses of the parameters	607:673	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	3	7	theme	corn	557:560	arg1	stover					562:567	differently pretreated corn stover	534:567	differently pretreated corn stover	534:567	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	5	8	theme	specific	986:993	arg1	area					1003:1006	specific surface area	986:1006	specific surface area	986:1006	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	9	theme	stover	812:817	arg1	properties					758:767	microstructure properties	743:767	microstructure properties	743:767	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	4	9	theme	stover	812:817	arg1	efficacy					795:802	enzymatic hydrolysis efficacy	774:802	enzymatic hydrolysis efficacy	774:802	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	4	9	theme	stover	812:817	arg1	composition					730:740	composition	730:740	composition	730:740	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	5	10	theme	enzyme-accessible	1022:1038	arg1	volume					1045:1050	enzyme-accessible pore volume	1022:1050	enzyme-accessible pore volume	1022:1050	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	11	theme	surface	1057:1063	arg1	area					1065:1068	surface area	1057:1068	surface area of cellulose	1057:1081	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	12	theme	different	825:833	arg1	types					835:839	different types	825:839	different types of pretreatments	825:856	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	4	13	theme	hydrolysis	784:793	arg1	efficacy					795:802	enzymatic hydrolysis efficacy	774:802	enzymatic hydrolysis efficacy	774:802	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	0	14	theme	stover	116:121	arg1	microstructure					68:81	microstructure	68:81	microstructure	68:81	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	0	14	theme	stover	116:121	arg1	hydrolysis					97:106	enzymatic hydrolysis	87:106	enzymatic hydrolysis	87:106	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	0	14	theme	stover	116:121	arg1	composition					55:65	composition	55:65	composition	55:65	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	5	15	theme	pore	1040:1043	arg1	volume					1045:1050	enzyme-accessible pore volume	1022:1050	enzyme-accessible pore volume	1022:1050	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	2	16	theme	corn	439:442	arg1	stover					444:449	corn stover	439:449	corn stover	439:449	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	2	17	theme	grinding	288:295	arg1	pretreatment					297:308	ultrafine grinding pretreatment	278:308	ultrafine grinding pretreatment	278:308	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	6	18	theme	correlation	1169:1179	arg1	analysis					1181:1188	quantitative correlation analysis	1156:1188	quantitative correlation analysis	1156:1188	The results of quantitative correlation analysis were GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21.
31473375	3	19	theme	lignocellulosic	456:470	arg1	composition					472:482	The lignocellulosic composition	452:482	The lignocellulosic composition	452:482	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	0	20	theme	corn	111:114	arg1	stover					116:121	corn stover	111:121	corn stover	111:121	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	2	21	theme	ultrafine	278:286	arg1	pretreatment					297:308	ultrafine grinding pretreatment	278:308	ultrafine grinding pretreatment	278:308	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	4	22	theme	parameters	664:673	arg1	analyses					648:655	qualitative and quantitative correlation analyses	607:655	qualitative and quantitative correlation analyses of the parameters	607:673	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	1	23	theme	key	181:183	arg1	step					185:188	a key step	179:188	a key step in the energy utilization of lignocellulosic biomass	179:241	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	1	23	theme	key	181:183	arg1	Pretreatment					163:174	Pretreatment	163:174	Pretreatment	163:174	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	3	24	theme	stover	562:567	arg1	yield					525:529	glucose yield	517:529	glucose yield	517:529	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	3	24	theme	stover	562:567	arg1	composition					472:482	The lignocellulosic composition	452:482	The lignocellulosic composition	452:482	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	3	24	theme	stover	562:567	arg1	parameters					501:510	microstructural parameters	485:510	microstructural parameters	485:510	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	4	25	theme	corn	807:810	arg1	stover					812:817	corn stover	807:817	corn stover after different types of pretreatments	807:856	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	5	26	theme	cellulose	967:975	arg1	content					977:983	cellulose content	967:983	cellulose content	967:983	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	2	27	theme	peroxide	330:337	arg1	pretreatment					339:350	alkaline hydroxide peroxide pretreatment	311:350	alkaline hydroxide peroxide pretreatment	311:350	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	2	28	theme	expansion	397:405	arg1	pretreatment					407:418	ammonia fiber expansion pretreatment	383:418	ammonia fiber expansion pretreatment	383:418	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	0	29	theme	correlation	25:35	arg1	analysis					37:44	intrinsic correlation analysis	15:44	intrinsic correlation analysis	15:44	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	5	30	theme	significant	1087:1097	arg1	correlations					1108:1119	significant positive correlations	1087:1119	significant positive correlations	1087:1119	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	0	31	theme	different	129:137	arg1	types					139:143	different types	129:143	different types of pretreatments	129:160	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	2	32	theme	hydroxide	320:328	arg1	pretreatment					339:350	alkaline hydroxide peroxide pretreatment	311:350	alkaline hydroxide peroxide pretreatment	311:350	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	0	33	theme	intrinsic	15:23	arg1	analysis					37:44	intrinsic correlation analysis	15:44	intrinsic correlation analysis	15:44	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	4	34	theme	pretreatments	844:856	arg1	types					835:839	different types	825:839	different types of pretreatments	825:856	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	2	35	theme	alkaline	311:318	arg1	pretreatment					339:350	alkaline hydroxide peroxide pretreatment	311:350	alkaline hydroxide peroxide pretreatment	311:350	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	1	36	theme	energy	197:202	arg1	utilization					204:214	the energy utilization	193:214	the energy utilization of lignocellulosic biomass	193:241	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	4	37	theme	qualitative	607:617	arg1	analyses					648:655	qualitative and quantitative correlation analyses	607:655	qualitative and quantitative correlation analyses of the parameters	607:673	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	2	38	theme	Different	244:252	arg1	types					254:258	Different types	244:258	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment)	244:419	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	2	39	dep	pretreatments	263:275	arg1	pretreatment					297:308	ultrafine grinding pretreatment	278:308	ultrafine grinding pretreatment	278:308	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	5	40	theme	correlation	935:945	arg1	analysis					947:954	Qualitative correlation analysis	923:954	Qualitative correlation analysis	923:954	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	41	theme	microstructure	743:756	arg1	properties					758:767	microstructure properties	743:767	microstructure properties	743:767	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	3	42	theme	microstructural	485:499	arg1	parameters					501:510	microstructural parameters	485:510	microstructural parameters	485:510	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	5	43	theme	glucose	1126:1132	arg1	yield					1134:1138	glucose yield	1126:1138	glucose yield	1126:1138	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	44	theme	Qualitative	923:933	arg1	analysis					947:954	Qualitative correlation analysis	923:954	Qualitative correlation analysis	923:954	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	45	theme	enzymatic	774:782	arg1	efficacy					795:802	enzymatic hydrolysis efficacy	774:802	enzymatic hydrolysis efficacy	774:802	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	6	46	theme	quantitative	1156:1167	arg1	analysis					1181:1188	quantitative correlation analysis	1156:1188	quantitative correlation analysis	1156:1188	The results of quantitative correlation analysis were GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21.
31473375	0	47	theme	pretreatments	148:160	arg1	types					139:143	different types	129:143	different types of pretreatments	129:160	Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments.
31473375	4	48	theme	enzymatic	901:909	arg1	hydrolysis					911:920	enzymatic hydrolysis	901:920	enzymatic hydrolysis	901:920	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	3	49	theme	pretreated	546:555	arg1	stover					562:567	differently pretreated corn stover	534:567	differently pretreated corn stover	534:567	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	2	50	theme	acid	360:363	arg1	pretreatment					365:376	dilute acid pretreatment	353:376	dilute acid pretreatment	353:376	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	5	51	theme	cellulose	1073:1081	arg1	volume					1045:1050	enzyme-accessible pore volume	1022:1050	enzyme-accessible pore volume	1022:1050	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	51	theme	cellulose	1073:1081	arg1	area					1065:1068	surface area	1057:1068	surface area of cellulose	1057:1081	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	51	theme	cellulose	1073:1081	arg1	area					1003:1006	specific surface area	986:1006	specific surface area	986:1006	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	51	theme	cellulose	1073:1081	arg1	volume					1014:1019	pore volume	1009:1019	pore volume	1009:1019	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	5	51	theme	cellulose	1073:1081	arg1	content					977:983	cellulose content	967:983	cellulose content	967:983	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	4	52	dep	composition	730:740	arg1	the					726:728	the	726:728	the	726:728	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	2	53	theme	dilute	353:358	arg1	pretreatment					365:376	dilute acid pretreatment	353:376	dilute acid pretreatment	353:376	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	1	54	theme	lignocellulosic	219:233	arg1	biomass					235:241	lignocellulosic biomass	219:241	lignocellulosic biomass	219:241	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	2	55	theme	pretreatments	263:275	arg1	types					254:258	Different types	244:258	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment)	244:419	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	1	56	from	step	185:188	arg1	utilization					204:214	the energy utilization	193:214	the energy utilization of lignocellulosic biomass	193:241	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	3	57	theme	glucose	517:523	arg1	yield					525:529	glucose yield	517:529	glucose yield	517:529	The lignocellulosic composition, microstructural parameters, and glucose yield of differently pretreated corn stover were characterized and compared.
31473375	1	58	theme	biomass	235:241	arg1	utilization					204:214	the energy utilization	193:214	the energy utilization of lignocellulosic biomass	193:241	Pretreatment is a key step in the energy utilization of lignocellulosic biomass.
31473375	4	59	theme	main	875:878	arg1	properties					880:889	the main properties	871:889	the main properties affecting enzymatic hydrolysis	871:920	Then qualitative and quantitative correlation analyses of the parameters were carried out to explore the correlations among the composition, microstructure properties, and enzymatic hydrolysis efficacy of corn stover after different types of pretreatments and identify the main properties affecting enzymatic hydrolysis.
31473375	2	60	theme	fiber	391:395	arg1	pretreatment					407:418	ammonia fiber expansion pretreatment	383:418	ammonia fiber expansion pretreatment	383:418	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31473375	6	61	theme	analysis	1181:1188	arg1	results					1145:1151	The results	1141:1151	The results of quantitative correlation analysis	1141:1188	The results of quantitative correlation analysis were GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21.
31473375	6	61	theme	analysis	1181:1188	arg1	GY = 15.01 × cellulose					1195:1216	GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21	1195:1344	GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21	1195:1344	The results of quantitative correlation analysis were GY = 15.01 × cellulose content-339.05, GY = 13.06 × SSA + 172.35, GY = 7226.27 × PV + 129.14, GY = 8628.61 × EAPV + 125.61, and GY = 1.18 × SAC-287.21.
31473375	5	62	theme	positive	1099:1106	arg1	correlations					1108:1119	significant positive correlations	1087:1119	significant positive correlations	1087:1119	Qualitative correlation analysis found that cellulose content, specific surface area, pore volume, enzyme-accessible pore volume, and surface area of cellulose had significant positive correlations with glucose yield.
31473375	2	63	theme	ammonia	383:389	arg1	pretreatment					407:418	ammonia fiber expansion pretreatment	383:418	ammonia fiber expansion pretreatment	383:418	Different types of pretreatments (ultrafine grinding pretreatment, alkaline hydroxide peroxide pretreatment, dilute acid pretreatment, and ammonia fiber expansion pretreatment) were conducted on corn stover.
31780773	0	0	theme	substances	107:116	arg1	assessment					21:30	assessment	21:30	assessment	21:30	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	0	0	theme	substances	107:116	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	6	1	theme	barnacle	1024:1031	arg1	larvae					1033:1038	the barnacle larvae	1020:1038	the barnacle larvae	1020:1038	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	6	2	with	treatment	1007:1015	arg1	EPSs					1045:1048	EPSs	1045:1048	EPSs from three bacterial strains	1045:1077	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	1	3	theme	biofilm	281:287	arg1	environment					289:299	a biofilm environment	279:299	a biofilm environment	279:299	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	0	4	theme	polymeric	97:105	arg1	substances					107:116	extracellular polymeric substances	83:116	extracellular polymeric substances isolated from marine biofilm bacteria	83:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	4	5	theme	ecological	525:534	arg1	significance					536:547	The ecological significance	521:547	The ecological significance of exopolymers	521:562	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	7	6	theme	invertebrate	1190:1201	arg1	settlement					1210:1219	the invertebrate larval settlement	1186:1219	the invertebrate larval settlement on artificial materials	1186:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	2	7	theme	bacteria	382:389	arg1	EPSs					353:356	EPSs	353:356	EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	353:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	4	8	theme	marine	591:596	arg1	larvae					622:627	marine model organism barnacle larvae	591:627	marine model organism barnacle larvae for their settlement-inducing activity	591:666	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	7	9	theme	artificial	1224:1233	arg1	materials					1235:1243	artificial materials	1224:1243	artificial materials	1224:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	4	10	theme	barnacle	613:620	arg1	larvae					622:627	marine model organism barnacle larvae	591:627	marine model organism barnacle larvae for their settlement-inducing activity	591:666	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	2	11	dep	bacteria	382:389	arg1	harveyi					434:440	Vibrio harveyi	427:440	Vibrio harveyi	427:440	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	11	dep	bacteria	382:389	arg1	sp					461:462	Planomicrobium sp	446:462	Planomicrobium sp	446:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	11	dep	bacteria	382:389	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	11	dep	bacteria	382:389	arg1	shioyasakiensis					410:424	Pseudoalteromonas shioyasakiensis	392:424	Pseudoalteromonas shioyasakiensis	392:424	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	4	12	theme	organism	604:611	arg1	larvae					622:627	marine model organism barnacle larvae	591:627	marine model organism barnacle larvae for their settlement-inducing activity	591:666	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	0	13	theme	biofilm	139:145	arg1	bacteria					147:154	marine biofilm bacteria	132:154	marine biofilm bacteria	132:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	2	14	dep	variation	335:343	arg1	study					310:314	this study	305:314	this study	305:314	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	1	15	theme	hydrated	207:214	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPSs)	157:197	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	15	theme	hydrated	207:214	arg1	matrix					227:232	the hydrated gelatinous matrix	203:232	the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment	203:299	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	5	16	theme	extracted	959:967	arg1	EPSs					969:972	all extracted EPSs	955:972	all extracted EPSs	955:972	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	0	17	theme	marine	132:137	arg1	bacteria					147:154	marine biofilm bacteria	132:154	marine biofilm bacteria	132:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	4	18	theme	model	598:602	arg1	larvae					622:627	marine model organism barnacle larvae	591:627	marine model organism barnacle larvae for their settlement-inducing activity	591:666	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	5	19	theme	Chemical	669:676	arg1	analysis					678:685	Chemical analysis	669:685	Chemical analysis	669:685	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	2	20	theme	Vibrio	427:432	arg1	harveyi					434:440	Vibrio harveyi	427:440	Vibrio harveyi	427:440	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	20	theme	Vibrio	427:432	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	5	21	theme	isoglobotriaose-β-N	914:932	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	21	theme	isoglobotriaose-β-N	914:932	arg1	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	7	22	theme	larval	1203:1208	arg1	settlement					1210:1219	the invertebrate larval settlement	1186:1219	the invertebrate larval settlement on artificial materials	1186:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	1	23	theme	gelatinous	216:225	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPSs)	157:197	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	23	theme	gelatinous	216:225	arg1	matrix					227:232	the hydrated gelatinous matrix	203:232	the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment	203:299	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	6	24	from	strains	1071:1077	arg1	EPSs					1045:1048	EPSs	1045:1048	EPSs from three bacterial strains	1045:1077	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	5	25	theme	Acetyl	934:939	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	25	theme	Acetyl	934:939	arg1	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	7	26	from	settlement	1210:1219	arg1	materials					1235:1243	artificial materials	1224:1243	artificial materials	1224:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	6	27	theme	Bioassay	975:982	arg1	results					984:990	Bioassay results	975:990	Bioassay results	975:990	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	2	28	theme	compositional	321:333	arg1	variation					335:343	the compositional variation	317:343	the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	317:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	0	29	theme	larval	44:49	arg1	activity					71:78	barnacle larval settlement-inducing activity	35:78	barnacle larval settlement-inducing activity	35:78	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	6	30	theme	bacterial	1061:1069	arg1	strains					1071:1077	three bacterial strains	1055:1077	three bacterial strains	1055:1077	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	7	31	theme	water-soluble	1164:1176	arg1	EPSs					1178:1181	water-soluble EPSs	1164:1181	water-soluble EPSs	1164:1181	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	0	32	theme	barnacle	35:42	arg1	activity					71:78	barnacle larval settlement-inducing activity	35:78	barnacle larval settlement-inducing activity	35:78	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	5	33	from	presence	700:707	arg1	EPSs					969:972	all extracted EPSs	955:972	all extracted EPSs	955:972	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	7	34	theme	EPSs	1178:1181	arg1	role					1156:1159	the role	1152:1159	the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials	1152:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	7	35	from	role	1156:1159	arg1	settlement					1210:1219	the invertebrate larval settlement	1186:1219	the invertebrate larval settlement on artificial materials	1186:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	7	35	from	role	1156:1159	arg1	materials					1235:1243	artificial materials	1224:1243	artificial materials	1224:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	2	36	theme	Planomicrobium	446:459	arg1	sp					461:462	Planomicrobium sp	446:462	Planomicrobium sp	446:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	36	theme	Planomicrobium	446:459	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	0	37	theme	activity	71:78	arg1	assessment					21:30	assessment	21:30	assessment	21:30	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	0	37	theme	activity	71:78	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	7	38	from	materials	1235:1243	arg1	role					1156:1159	the role	1152:1159	the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials	1152:1243	In conclusion, this study highlighted the role of water-soluble EPSs in the invertebrate larval settlement on artificial materials.
31780773	5	39	theme	glycan	712:717	arg1	N-acetyllactosamine					890:908	3'-sialyl N-acetyllactosamine	880:908	3'-sialyl N-acetyllactosamine	880:908	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	trisaccharides					759:772	trisaccharides	759:772	trisaccharides	759:772	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	oligomannose					814:825	oligomannose	814:825	oligomannose	814:825	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	galacto-N-biose					828:842	galacto-N-biose	828:842	galacto-N-biose	828:842	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	iso-B-Pentasaccharides					775:796	iso-B-Pentasaccharides	775:796	iso-B-Pentasaccharides	775:796	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	sialyllactose					799:811	sialyllactose	799:811	sialyllactose	799:811	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	difucosyl-para-lacto-N-neohexaose					845:877	difucosyl-para-lacto-N-neohexaose	845:877	difucosyl-para-lacto-N-neohexaose	845:877	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	39	theme	glycan	712:717	arg1	tetraose					749:756	tetraose	749:756	tetraose	749:756	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	2	40	theme	biofilm	374:380	arg1	harveyi					434:440	Vibrio harveyi	427:440	Vibrio harveyi	427:440	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	40	theme	biofilm	374:380	arg1	sp					461:462	Planomicrobium sp	446:462	Planomicrobium sp	446:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	40	theme	biofilm	374:380	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	40	theme	biofilm	374:380	arg1	shioyasakiensis					410:424	Pseudoalteromonas shioyasakiensis	392:424	Pseudoalteromonas shioyasakiensis	392:424	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	0	41	theme	settlement-inducing	51:69	arg1	activity					71:78	barnacle larval settlement-inducing activity	35:78	barnacle larval settlement-inducing activity	35:78	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	5	42	theme	fucosylated	719:729	arg1	N-acetyllactosamine					890:908	3'-sialyl N-acetyllactosamine	880:908	3'-sialyl N-acetyllactosamine	880:908	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	trisaccharides					759:772	trisaccharides	759:772	trisaccharides	759:772	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	oligomannose					814:825	oligomannose	814:825	oligomannose	814:825	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	galacto-N-biose					828:842	galacto-N-biose	828:842	galacto-N-biose	828:842	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	iso-B-Pentasaccharides					775:796	iso-B-Pentasaccharides	775:796	iso-B-Pentasaccharides	775:796	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	sialyllactose					799:811	sialyllactose	799:811	sialyllactose	799:811	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	difucosyl-para-lacto-N-neohexaose					845:877	difucosyl-para-lacto-N-neohexaose	845:877	difucosyl-para-lacto-N-neohexaose	845:877	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	42	theme	fucosylated	719:729	arg1	tetraose					749:756	tetraose	749:756	tetraose	749:756	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	2	43	theme	marine	367:372	arg1	harveyi					434:440	Vibrio harveyi	427:440	Vibrio harveyi	427:440	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	43	theme	marine	367:372	arg1	sp					461:462	Planomicrobium sp	446:462	Planomicrobium sp	446:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	43	theme	marine	367:372	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	43	theme	marine	367:372	arg1	shioyasakiensis					410:424	Pseudoalteromonas shioyasakiensis	392:424	Pseudoalteromonas shioyasakiensis	392:424	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	4	44	theme	exopolymers	552:562	arg1	significance					536:547	The ecological significance	521:547	The ecological significance of exopolymers	521:562	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	5	45	theme	oligosaccharides	731:746	arg1	presence					700:707	the presence	696:707	the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs	696:972	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	1	46	theme	Extracellular	157:169	arg1	EPSs					193:196	EPSs	193:196	EPSs	193:196	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	46	theme	Extracellular	157:169	arg1	matrix					227:232	the hydrated gelatinous matrix	203:232	the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment	203:299	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	46	theme	Extracellular	157:169	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPSs)	157:197	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	0	47	attach	isolated	118:125	arg1	bacteria					147:154	marine biofilm bacteria	132:154	marine biofilm bacteria	132:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	0	47	attach	isolated	118:125	arg2	substances					107:116	extracellular polymeric substances	83:116	extracellular polymeric substances isolated from marine biofilm bacteria	83:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	1	48	theme	polymeric	171:179	arg1	EPSs					193:196	EPSs	193:196	EPSs	193:196	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	48	theme	polymeric	171:179	arg1	matrix					227:232	the hydrated gelatinous matrix	203:232	the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment	203:299	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	1	48	theme	polymeric	171:179	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPSs)	157:197	Extracellular polymeric substances (EPSs) are the hydrated gelatinous matrix produced by microorganisms for attachment in a biofilm environment.
31780773	0	49	theme	extracellular	83:95	arg1	substances					107:116	extracellular polymeric substances	83:116	extracellular polymeric substances isolated from marine biofilm bacteria	83:154	Characterization and assessment of barnacle larval settlement-inducing activity of extracellular polymeric substances isolated from marine biofilm bacteria.
31780773	3	50	theme	1H	490:491	arg1	NMR					493:495	1H NMR	490:495	1H NMR	490:495	were analysed by GC-MS, 1H NMR, FT-IR and XRD and SEM.
31780773	5	51	gly	fucosylated	719:729	arg1	N-acetyllactosamine					890:908	3'-sialyl N-acetyllactosamine	880:908	3'-sialyl N-acetyllactosamine	880:908	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	trisaccharides					759:772	trisaccharides	759:772	trisaccharides	759:772	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	oligomannose					814:825	oligomannose	814:825	oligomannose	814:825	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	galacto-N-biose					828:842	galacto-N-biose	828:842	galacto-N-biose	828:842	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	iso-B-Pentasaccharides					775:796	iso-B-Pentasaccharides	775:796	iso-B-Pentasaccharides	775:796	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	sialyllactose					799:811	sialyllactose	799:811	sialyllactose	799:811	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	difucosyl-para-lacto-N-neohexaose					845:877	difucosyl-para-lacto-N-neohexaose	845:877	difucosyl-para-lacto-N-neohexaose	845:877	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	51	gly	fucosylated	719:729	arg1	tetraose					749:756	tetraose	749:756	tetraose	749:756	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	iso-B-Pentasaccharides					775:796	iso-B-Pentasaccharides	775:796	iso-B-Pentasaccharides	775:796	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	tetraose					749:756	tetraose	749:756	tetraose	749:756	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	sialyllactose					799:811	sialyllactose	799:811	sialyllactose	799:811	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	difucosyl-para-lacto-N-neohexaose					845:877	difucosyl-para-lacto-N-neohexaose	845:877	difucosyl-para-lacto-N-neohexaose	845:877	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	trisaccharides					759:772	trisaccharides	759:772	trisaccharides	759:772	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	N-acetyllactosamine					890:908	3'-sialyl N-acetyllactosamine	880:908	3'-sialyl N-acetyllactosamine	880:908	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	oligomannose					814:825	oligomannose	814:825	oligomannose	814:825	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	-Propargyl					941:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	isoglobotriaose-β-N(Acetyl)-Propargyl	914:950	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg1	EPSs					969:972	all extracted EPSs	955:972	all extracted EPSs	955:972	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	52	attach	presence	700:707	arg2	galacto-N-biose					828:842	galacto-N-biose	828:842	galacto-N-biose	828:842	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	4	53	theme	settlement-inducing	639:657	arg1	activity					659:666	their settlement-inducing activity	633:666	their settlement-inducing activity	633:666	The ecological significance of exopolymers was assessed in vivo using marine model organism barnacle larvae for their settlement-inducing activity.
31780773	5	54	theme	3'-sialyl	880:888	arg1	oligosaccharides					731:746	glycan fucosylated oligosaccharides	712:746	glycan fucosylated oligosaccharides	712:746	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	5	54	theme	3'-sialyl	880:888	arg1	N-acetyllactosamine					890:908	3'-sialyl N-acetyllactosamine	880:908	3'-sialyl N-acetyllactosamine	880:908	Chemical analysis revealed the presence of glycan fucosylated oligosaccharides, tetraose, trisaccharides, iso-B-Pentasaccharides, sialyllactose, oligomannose, galacto-N-biose, difucosyl-para-lacto-N-neohexaose, 3'-sialyl N-acetyllactosamine and isoglobotriaose-β-N(Acetyl)-Propargyl in all extracted EPSs.
31780773	6	55	theme	larvae	1033:1038	arg1	treatment					1007:1015	treatment	1007:1015	treatment of the barnacle larvae with EPSs from three bacterial strains	1007:1077	Bioassay results indicated that treatment of the barnacle larvae with EPSs from three bacterial strains enhanced settlement on substrates.
31780773	2	56	theme	Pseudoalteromonas	392:408	arg1	bacteria					382:389	three marine biofilm bacteria	361:389	three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp	361:462	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
31780773	2	56	theme	Pseudoalteromonas	392:408	arg1	shioyasakiensis					410:424	Pseudoalteromonas shioyasakiensis	392:424	Pseudoalteromonas shioyasakiensis	392:424	In this study, the compositional variation between EPSs of three marine biofilm bacteria (Pseudoalteromonas shioyasakiensis, Vibrio harveyi and Planomicrobium sp.)
30579102	3	0	theme	different	607:615	arg1	solvents					617:624	three different solvents	601:624	three different solvents	601:624	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	0	1	theme	methanol	77:84	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	2	2	theme	different	362:370	arg1	temperatures					372:383	different temperatures	362:383	different temperatures (240, 260, 280, 300 and 320 °C)	362:415	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	3	dep	dosage	473:478	arg1	200 mL					501:506	200 mL	501:506	200 mL	501:506	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	3	dep	dosage	473:478	arg1	160					493:495	160	493:495	160	493:495	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	0	4	from	Effects	0:6	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	0	4	from	Effects	0:6	arg1	products					110:117	products	110:117	products	110:117	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	2	5	dep	temperatures	372:383	arg1	300					401:403	300	401:403	300	401:403	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	5	dep	temperatures	372:383	arg1	dosage					473:478	dosage (0, 80, 120	473:490	dosage	473:478	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	5	dep	temperatures	372:383	arg1	320 °C					409:414	320 °C	409:414	320 °C	409:414	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	0	6	theme	ethanol	87:93	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	4	7	theme	residue	664:670	arg1	yield					672:676	smaller residue yield	656:676	smaller residue yield	656:676	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	6	8	theme	bio-oil	939:945	arg1	hydrocarbon					996:1006	hydrocarbon	996:1006	hydrocarbon	996:1006	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	6	8	theme	bio-oil	939:945	arg1	constituents					923:934	The dominant constituents	910:934	The dominant constituents of bio-oil that were produced in supercritical methanol	910:990	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	6	9	theme	supercritical	969:981	arg1	methanol					983:990	supercritical methanol	969:990	supercritical methanol	969:990	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	0	10	theme	acetone	99:105	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	4	11	theme	suitable	751:758	arg1	conditions					760:769	the suitable conditions	747:769	the suitable conditions (320 °C, 160 mL, 60-90 min)	747:797	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	6	12	theme	supercritical	1056:1068	arg1	ethanol					1070:1076	supercritical ethanol	1056:1076	supercritical ethanol	1056:1076	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	5	13	theme	solvents	830:837	arg1	type					822:825	the type	818:825	the type of solvents	818:837	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	5	13	theme	solvents	830:837	arg1	effect					852:857	the main effect	843:857	the main effect	843:857	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	2	14	theme	reaction	418:425	arg1	120 min					451:457	0, 30, 60, 90 and 120 min	433:457	0, 30, 60, 90 and 120 min	433:457	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	14	theme	reaction	418:425	arg1	time					427:430	reaction time	418:430	reaction time (0, 30, 60, 90 and 120 min)	418:458	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	4	15	dep	yield	646:650	arg1	The					627:629	The	627:629	The	627:629	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	4	16	theme	bio-oil	638:644	arg1	yield					646:650	larger bio-oil yield	631:650	larger bio-oil yield	631:650	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	2	17	dep	solvents	282:289	arg1	ethanol					302:308	ethanol	302:308	ethanol	302:308	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	17	dep	solvents	282:289	arg1	methanol					292:299	methanol	292:299	methanol	292:299	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	17	dep	solvents	282:289	arg1	solvents					282:289	Different organic solvents	264:289	Different organic solvents (methanol, ethanol and acetone)	264:321	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	17	dep	solvents	282:289	arg1	acetone					314:320	acetone	314:320	acetone	314:320	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	0	18	theme	parameters	24:33	arg1	Effects					0:6	Effects	0:6	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products	0:117	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	5	19	theme	composition	886:896	arg1	distribution					866:877	the distribution	862:877	the distribution of the composition of bio-oil	862:907	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	4	20	theme	smaller	656:662	arg1	yield					672:676	smaller residue yield	656:676	smaller residue yield	656:676	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	1	21	theme	supercritical	195:207	arg1	solvents					217:224	supercritical organic solvents	195:224	supercritical organic solvents	195:224	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	0	22	theme	liquefaction	11:22	arg1	parameters					24:33	liquefaction parameters	11:33	liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone	11:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	3	23	theme	similar	534:540	arg1	characteristics					555:569	similar liquefaction characteristics	534:569	similar liquefaction characteristics of cellulose	534:582	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	5	24	theme	main	847:850	arg1	type					822:825	the type	818:825	the type of solvents	818:837	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	5	24	theme	main	847:850	arg1	effect					852:857	the main effect	843:857	the main effect	843:857	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	1	25	theme	organic	209:215	arg1	solvents					217:224	supercritical organic solvents	195:224	supercritical organic solvents	195:224	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	0	26	theme	cellulose	38:46	arg1	parameters					24:33	liquefaction parameters	11:33	liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone	11:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	3	27	theme	liquefaction	542:553	arg1	characteristics					555:569	similar liquefaction characteristics	534:569	similar liquefaction characteristics of cellulose	534:582	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	5	28	theme	bio-oil	901:907	arg1	composition					886:896	the composition	882:896	the composition of bio-oil	882:907	And the type of solvents was the main effect to the distribution of the composition of bio-oil.
30579102	4	29	dep	conditions	760:769	arg1	160 mL					780:785	160 mL	780:785	160 mL	780:785	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	4	29	dep	conditions	760:769	arg1	320 °C					772:777	320 °C	772:777	320 °C	772:777	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	4	29	dep	conditions	760:769	arg1	60-90 min					788:796	60-90 min	788:796	60-90 min	788:796	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	1	30	from	solvents	217:224	arg1	liquefied					182:190	liquefied	182:190	liquefied	182:190	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	3	31	located	observed	589:596	arg2	characteristics					555:569	similar liquefaction characteristics	534:569	similar liquefaction characteristics of cellulose	534:582	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	3	31	located	observed	589:596	arg1	solvents					617:624	three different solvents	601:624	three different solvents	601:624	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	0	32	theme	supercritical	51:63	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	1	33	from	parameters	252:261	arg1	liquefied					182:190	liquefied	182:190	liquefied	182:190	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	0	34	from	solvents	65:72	arg1	Effects					0:6	Effects	0:6	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products	0:117	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	3	35	theme	cellulose	574:582	arg1	characteristics					555:569	similar liquefaction characteristics	534:569	similar liquefaction characteristics of cellulose	534:582	The results showed that similar liquefaction characteristics of cellulose were observed in three different solvents.
30579102	6	36	dep	hydrocarbon	996:1006	arg1	whereas					1079:1085	whereas	1079:1085	whereas	1079:1085	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	4	37	theme	larger	631:636	arg1	yield					646:650	larger bio-oil yield	631:650	larger bio-oil yield	631:650	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	6	38	theme	ketones	1087:1093	arg1	compounds					1095:1103	ketones compounds	1087:1103	ketones compounds	1087:1103	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	6	39	theme	supercritical	1131:1143	arg1	acetone					1145:1151	supercritical acetone	1131:1151	supercritical acetone	1131:1151	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	2	40	theme	disintegrate	336:347	arg1	cellulose					349:357	disintegrate cellulose	336:357	disintegrate cellulose	336:357	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	1	41	theme	different	229:237	arg1	parameters					252:261	different liquefaction parameters	229:261	different liquefaction parameters	229:261	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	4	42	theme	cellulose	692:700	arg1	liquefaction					702:713	cellulose liquefaction	692:713	cellulose liquefaction in solvents	692:725	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	1	43	from	liquefied	182:190	arg1	solvents					217:224	supercritical organic solvents	195:224	supercritical organic solvents	195:224	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	1	44	theme	liquefaction	239:250	arg1	parameters					252:261	different liquefaction parameters	229:261	different liquefaction parameters	229:261	Cellulose extracted from cornstalk was liquefied in supercritical organic solvents at different liquefaction parameters.
30579102	2	45	theme	organic	274:280	arg1	ethanol					302:308	ethanol	302:308	ethanol	302:308	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	45	theme	organic	274:280	arg1	methanol					292:299	methanol	292:299	methanol	292:299	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	45	theme	organic	274:280	arg1	solvents					282:289	Different organic solvents	264:289	Different organic solvents (methanol, ethanol and acetone)	264:321	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	45	theme	organic	274:280	arg1	acetone					314:320	acetone	314:320	acetone	314:320	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	46	used	used	328:331	arg2	methanol					292:299	methanol	292:299	methanol	292:299	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	46	used	used	328:331	arg2	solvents					282:289	Different organic solvents	264:289	Different organic solvents (methanol, ethanol and acetone)	264:321	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	46	used	used	328:331	arg2	acetone					314:320	acetone	314:320	acetone	314:320	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	46	used	used	328:331	arg2	ethanol					302:308	ethanol	302:308	ethanol	302:308	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	0	47	from	parameters	24:33	arg1	solvents					65:72	supercritical solvents	51:72	supercritical solvents of methanol, ethanol and acetone	51:105	Effects of liquefaction parameters of cellulose in supercritical solvents of methanol, ethanol and acetone on products yield and compositions.
30579102	2	48	theme	Different	264:272	arg1	ethanol					302:308	ethanol	302:308	ethanol	302:308	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	48	theme	Different	264:272	arg1	methanol					292:299	methanol	292:299	methanol	292:299	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	48	theme	Different	264:272	arg1	solvents					282:289	Different organic solvents	264:289	Different organic solvents (methanol, ethanol and acetone)	264:321	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	2	48	theme	Different	264:272	arg1	acetone					314:320	acetone	314:320	acetone	314:320	Different organic solvents (methanol, ethanol and acetone) were used to disintegrate cellulose at different temperatures (240, 260, 280, 300 and 320 °C), reaction time (0, 30, 60, 90 and 120 min) and solvents dosage (0, 80, 120, 160 and 200 mL).
30579102	4	49	from	liquefaction	702:713	arg1	solvents					718:725	solvents	718:725	solvents	718:725	The larger bio-oil yield and smaller residue yield obtained from cellulose liquefaction in solvents were achieved under the suitable conditions (320 °C, 160 mL, 60-90 min), respectively.
30579102	6	50	theme	dominant	914:921	arg1	hydrocarbon					996:1006	hydrocarbon	996:1006	hydrocarbon	996:1006	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30579102	6	50	theme	dominant	914:921	arg1	constituents					923:934	The dominant constituents	910:934	The dominant constituents of bio-oil that were produced in supercritical methanol	910:990	The dominant constituents of bio-oil that were produced in supercritical methanol was hydrocarbon, and ketones and esters were mainly produced in supercritical ethanol, whereas ketones compounds were primarily derived in supercritical acetone.
30707396	5	0	theme	strain	721:726	arg1	RS1T					728:731	strain RS1T	721:731	strain RS1T	721:731	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	6	1	theme	acids	870:874	arg1	acids					870:874	total fatty acids	858:874	total fatty acids	858:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	1	theme	acids	870:874	arg1	%					853:853	more than 10%	841:853	more than 10% of total fatty acids	841:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	7	2	theme	only	988:991	arg1	quinone					1004:1010	The only isoprenoid quinone	984:1010	The only isoprenoid quinone detected	984:1019	The only isoprenoid quinone detected was MK-7.
30707396	7	2	theme	only	988:991	arg1	MK-7					1025:1028	MK-7	1025:1028	MK-7	1025:1028	The only isoprenoid quinone detected was MK-7.
30707396	6	3	dep	C16:0	908:912	arg1	C16					951:953	C16	951:953	C16: 1ω7c and/or iso-C15:02-OH	951:980	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	4	theme	fatty	828:832	arg1	iso-C15					896:902	iso-C15	896:902	iso-C15	896:902	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	4	theme	fatty	828:832	arg1	acids					834:838	The major cellular fatty acids	809:838	The major cellular fatty acids (more than 10% of total fatty acids)	809:875	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	9	5	theme	62785T = GDMCC	1278:1291	arg1	1.1414T					1293:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).
30707396	3	6	theme	Phylogenetic	319:330	arg1	analysis					332:339	Phylogenetic analysis	319:339	Phylogenetic analysis based on 16S rRNA gene sequences	319:372	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	7	7	theme	isoprenoid	993:1002	arg1	quinone					1004:1010	The only isoprenoid quinone	984:1010	The only isoprenoid quinone detected	984:1019	The only isoprenoid quinone detected was MK-7.
30707396	7	7	theme	isoprenoid	993:1002	arg1	MK-7					1025:1028	MK-7	1025:1028	MK-7	1025:1028	The only isoprenoid quinone detected was MK-7.
30707396	6	8	dep	acids	834:838	arg1	acids					870:874	total fatty acids	858:874	total fatty acids	858:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	8	dep	acids	834:838	arg1	%					853:853	more than 10%	841:853	more than 10% of total fatty acids	841:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	3	9	theme	rRNA	354:357	arg1	sequences					364:372	16S rRNA gene sequences	350:372	16S rRNA gene sequences	350:372	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	4	10	theme	DNA	587:589	arg1	content					597:603	The DNA G + C content	583:603	The DNA G + C content of strain RS1T	583:618	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	4	10	theme	DNA	587:589	arg1	%					650:650	42.80 mol%	641:650	42.80 mol%	641:650	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	8	11	theme	physiological	1044:1056	arg1	characteristics					1081:1095	the physiological, genotypic and genomic characteristics	1040:1095	the physiological, genotypic and genomic characteristics	1040:1095	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	1	12	theme	polysaccharide-producing	54:77	arg1	bacterium					79:87	an endophytic polysaccharide-producing bacterium	40:87	an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis	40:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	6	13	dep	C16	951:953	arg1	iso-C15:02-OH					968:980	iso-C15:02-OH	968:980	iso-C15:02-OH	968:980	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	13	dep	C16	951:953	arg1	1ω7c					956:959	1ω7c	956:959	1ω7c	956:959	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	3	14	theme	DSM	562:564	arg1	%					579:579	97.36%	574:579	97.36%	574:579	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	14	theme	DSM	562:564	arg1	19975T					566:571	DSM 19975T	562:571	Mucilaginibacter oryzae DSM 19975T (97.36%)	538:580	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	2	15	theme	Gram-negative	151:163	arg1	strain					199:204	a Gram-negative, rod-shaped, endophytic bacterial strain	149:204	a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides	149:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	15	theme	Gram-negative	151:163	arg1	RS1T					207:210	RS1T	207:210	RS1T	207:210	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	8	16	theme	name	1197:1200	arg1	sp					1232:1233	the name Mucilaginibacter endophyticus sp	1193:1233	the name Mucilaginibacter endophyticus sp	1193:1233	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	8	17	theme	genus	1159:1163	arg1	Mucilaginibacter					1165:1180	the genus Mucilaginibacter	1155:1180	the genus Mucilaginibacter	1155:1180	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	3	18	dep	related	411:417	arg1	followed					476:483	followed	476:483	followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%)	476:580	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	19	theme	kameinonensis	439:451	arg1	102645T					458:464	Mucilaginibacter kameinonensis NBRC 102645T	422:464	Mucilaginibacter kameinonensis NBRC 102645T (98.72%)	422:473	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	19	theme	kameinonensis	439:451	arg1	%					472:472	98.72%	467:472	98.72%	467:472	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	20	theme	16S	350:352	arg1	sequences					364:372	16S rRNA gene sequences	350:372	16S rRNA gene sequences	350:372	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	6	21	theme	major	813:817	arg1	iso-C15					896:902	iso-C15	896:902	iso-C15	896:902	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	21	theme	major	813:817	arg1	acids					834:838	The major cellular fatty acids	809:838	The major cellular fatty acids (more than 10% of total fatty acids)	809:875	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	8	22	theme	endophyticus	1219:1230	arg1	sp					1232:1233	the name Mucilaginibacter endophyticus sp	1193:1233	the name Mucilaginibacter endophyticus sp	1193:1233	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	0	23	theme	Mucilaginibacter	0:15	arg1	sp					30:31	Mucilaginibacter endophyticus sp	0:31	Mucilaginibacter endophyticus sp.	0:32	Mucilaginibacter endophyticus sp.
30707396	6	24	dep	identified	882:891	arg1	iso-C17:0-3OH					915:927	iso-C17:0-3OH	915:927	iso-C17:0-3OH	915:927	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	24	dep	identified	882:891	arg1	feature					940:946	summed feature 3	933:948	summed feature 3	933:948	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	24	dep	identified	882:891	arg1	C16:0					908:912	C16:0	908:912	C16:0	908:912	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	24	dep	identified	882:891	arg1	0					905:905	0	905:905	0	905:905	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	3	25	theme	NBRC	453:456	arg1	102645T					458:464	Mucilaginibacter kameinonensis NBRC 102645T	422:464	Mucilaginibacter kameinonensis NBRC 102645T (98.72%)	422:473	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	25	theme	NBRC	453:456	arg1	%					472:472	98.72%	467:472	98.72%	467:472	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	0	26	theme	endophyticus	17:28	arg1	sp					30:31	Mucilaginibacter endophyticus sp	0:31	Mucilaginibacter endophyticus sp.	0:32	Mucilaginibacter endophyticus sp.
30707396	5	27	theme	RS1T	728:731	arg1	species					759:765	the related reference species	737:765	the related reference species	737:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	5	27	theme	RS1T	728:731	arg1	genomes					710:716	the genomes	706:716	the genomes of strain RS1T	706:731	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	2	28	theme	sinensis	309:316	arg1	stem					290:293	a stem	288:293	a stem of Miscanthus sinensis	288:316	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	6	29	theme	cellular	819:826	arg1	iso-C15					896:902	iso-C15	896:902	iso-C15	896:902	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	29	theme	cellular	819:826	arg1	acids					834:838	The major cellular fatty acids	809:838	The major cellular fatty acids (more than 10% of total fatty acids)	809:875	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	9	30	dep	proposed	1244:1251	arg1	1.1414T					1293:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).
30707396	3	31	theme	strain	388:393	arg1	RS1T					395:398	strain RS1T	388:398	strain RS1T	388:398	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	2	32	theme	large	234:238	arg1	amounts					240:246	large amounts	234:246	large amounts of exopolysaccharides	234:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	32	theme	large	234:238	arg1	exopolysaccharides					251:268	exopolysaccharides	251:268	exopolysaccharides	251:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	33	theme	Miscanthus	298:307	arg1	sinensis					309:316	Miscanthus sinensis	298:316	Miscanthus sinensis	298:316	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	3	34	theme	Mucilaginibacter	422:437	arg1	102645T					458:464	Mucilaginibacter kameinonensis NBRC 102645T	422:464	Mucilaginibacter kameinonensis NBRC 102645T (98.72%)	422:473	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	34	theme	Mucilaginibacter	422:437	arg1	%					472:472	98.72%	467:472	98.72%	467:472	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	8	35	theme	genotypic	1059:1067	arg1	characteristics					1081:1095	the physiological, genotypic and genomic characteristics	1040:1095	the physiological, genotypic and genomic characteristics	1040:1095	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	2	36	dep	Gram-negative	151:163	arg1	endophytic					178:187	endophytic	178:187	endophytic	178:187	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	36	dep	Gram-negative	151:163	arg1	rod-shaped					166:175	rod-shaped	166:175	rod-shaped	166:175	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	5	37	theme	GGDC	662:665	arg1	values					667:672	ANIb and GGDC values	653:672	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species	653:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	1	38	theme	Miscanthus	113:122	arg1	sinensis					124:131	Miscanthus sinensis	113:131	Miscanthus sinensis	113:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	6	39	theme	summed	933:938	arg1	feature					940:946	summed feature 3	933:948	summed feature 3	933:948	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	6	39	theme	summed	933:938	arg1	0					905:905	0	905:905	0	905:905	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	1	40	attach	isolated	89:96	arg1	stem					105:108	a stem	103:108	a stem of Miscanthus sinensis	103:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	1	40	attach	isolated	89:96	arg2	bacterium					79:87	an endophytic polysaccharide-producing bacterium	40:87	an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis	40:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	6	41	theme	fatty	864:868	arg1	acids					870:874	total fatty acids	858:874	total fatty acids	858:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	4	42	theme	RS1T	615:618	arg1	content					597:603	The DNA G + C content	583:603	The DNA G + C content of strain RS1T	583:618	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	4	42	theme	RS1T	615:618	arg1	%					650:650	42.80 mol%	641:650	42.80 mol%	641:650	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	8	43	theme	genomic	1073:1079	arg1	characteristics					1081:1095	the physiological, genotypic and genomic characteristics	1040:1095	the physiological, genotypic and genomic characteristics	1040:1095	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	6	44	theme	total	858:862	arg1	acids					870:874	total fatty acids	858:874	total fatty acids	858:874	The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH).
30707396	9	45	theme	strain	1259:1264	arg1	1.1414T					1293:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).
30707396	4	46	theme	strain	608:613	arg1	RS1T					615:618	strain RS1T	608:618	strain RS1T	608:618	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	5	47	theme	related	741:747	arg1	species					759:765	the related reference species	737:765	the related reference species	737:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	5	48	theme	genomic	679:685	arg1	comparison					687:696	genomic comparison	679:696	genomic comparison between the genomes of strain RS1T and the related reference species	679:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	5	49	from	comparison	687:696	arg1	values					667:672	ANIb and GGDC values	653:672	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species	653:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	2	50	theme	capable	213:219	arg1	strain					199:204	a Gram-negative, rod-shaped, endophytic bacterial strain	149:204	a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides	149:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	50	theme	capable	213:219	arg1	RS1T					207:210	RS1T	207:210	RS1T	207:210	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	1	51	theme	sinensis	124:131	arg1	stem					105:108	a stem	103:108	a stem of Miscanthus sinensis	103:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	5	52	theme	reference	749:757	arg1	species					759:765	the related reference species	737:765	the related reference species	737:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	2	53	attach	isolated	274:281	arg2	RS1T					207:210	RS1T	207:210	RS1T	207:210	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	53	attach	isolated	274:281	arg2	strain					199:204	a Gram-negative, rod-shaped, endophytic bacterial strain	149:204	a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides	149:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	53	attach	isolated	274:281	arg1	stem					290:293	a stem	288:293	a stem of Miscanthus sinensis	288:316	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	54	theme	exopolysaccharides	251:268	arg1	amounts					240:246	large amounts	234:246	large amounts of exopolysaccharides	234:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	54	theme	exopolysaccharides	251:268	arg1	exopolysaccharides					251:268	exopolysaccharides	251:268	exopolysaccharides	251:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	8	55	theme	Mucilaginibacter	1165:1180	arg1	species					1144:1150	a novel species	1136:1150	a novel species	1136:1150	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	8	56	theme	novel	1138:1142	arg1	species					1144:1150	a novel species	1136:1150	a novel species	1136:1150	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	5	57	theme	ANIb	653:656	arg1	values					667:672	ANIb and GGDC values	653:672	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species	653:765	ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively.
30707396	8	58	theme	Mucilaginibacter	1202:1217	arg1	sp					1232:1233	the name Mucilaginibacter endophyticus sp	1193:1233	the name Mucilaginibacter endophyticus sp	1193:1233	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	1	59	dep	bacterium	79:87	arg1	nov.					34:37	nov.	34:37	nov.	34:37	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	9	60	theme	type	1254:1257	arg1	1.1414T					1293:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).
30707396	2	61	theme	bacterial	189:197	arg1	strain					199:204	a Gram-negative, rod-shaped, endophytic bacterial strain	149:204	a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides	149:268	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	2	61	theme	bacterial	189:197	arg1	RS1T					207:210	RS1T	207:210	RS1T	207:210	In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis.
30707396	3	62	dep	Mucilaginibacter	538:553	arg1	%					579:579	97.36%	574:579	97.36%	574:579	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	62	dep	Mucilaginibacter	538:553	arg1	oryzae					555:560	Mucilaginibacter oryzae DSM 19975T (97.36%)	538:580	Mucilaginibacter oryzae DSM 19975T (97.36%)	538:580	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	3	62	dep	Mucilaginibacter	538:553	arg1	19975T					566:571	DSM 19975T	562:571	Mucilaginibacter oryzae DSM 19975T (97.36%)	538:580	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
30707396	4	63	theme	G + C	591:595	arg1	content					597:603	The DNA G + C content	583:603	The DNA G + C content of strain RS1T	583:618	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	4	63	theme	G + C	591:595	arg1	%					650:650	42.80 mol%	641:650	42.80 mol%	641:650	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	8	64	theme	strain	1098:1103	arg1	RS1T					1105:1108	strain RS1T	1098:1108	strain RS1T	1098:1108	Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp.
30707396	1	65	theme	endophytic	43:52	arg1	bacterium					79:87	an endophytic polysaccharide-producing bacterium	40:87	an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis	40:131	nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis.
30707396	4	66	theme	42.80 mol	641:649	arg1	content					597:603	The DNA G + C content	583:603	The DNA G + C content of strain RS1T	583:618	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	4	66	theme	42.80 mol	641:649	arg1	%					650:650	42.80 mol%	641:650	42.80 mol%	641:650	The DNA G + C content of strain RS1T was determined to be 42.80 mol%.
30707396	9	67	theme	RS1T = KCTC	1266:1276	arg1	1.1414T					1293:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	type strain RS1T = KCTC 62785T = GDMCC 1.1414T	1254:1299	nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).
30707396	3	68	theme	gene	359:362	arg1	sequences					364:372	16S rRNA gene sequences	350:372	16S rRNA gene sequences	350:372	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%).
29673955	4	0	theme	novel	504:508	arg1	scaffold					521:528	The novel COL-HA-MFC scaffold	500:528	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC	500:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	7	1	theme	mechanical	1067:1076	arg1	property					1078:1085	mechanical property	1067:1085	mechanical property	1067:1085	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	12	2	theme	hemolysis	1684:1692	arg1	%					1702:1702	≦5%	1700:1702	≦5%	1700:1702	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	2	theme	hemolysis	1684:1692	arg1	rate					1694:1697	suitable hemolysis rate	1675:1697	suitable hemolysis rate (≦5%)	1675:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	4	3	theme	cold	589:592	arg1	technique					613:621	cold isostatic pressing technique	589:621	cold isostatic pressing technique	589:621	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	7	4	theme	COL-HA-MFC	1109:1118	arg1	composites					1120:1129	COL-HA-MFC composites	1109:1129	COL-HA-MFC composites	1109:1129	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	6	5	theme	Ultraviolet-visible	875:893	arg1	spectrophotometer					895:911	Ultraviolet-visible spectrophotometer	875:911	Ultraviolet-visible spectrophotometer (UV)	875:916	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	6	5	theme	Ultraviolet-visible	875:893	arg1	UV					914:915	UV	914:915	UV	914:915	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	11	6	theme	nature	1551:1556	arg1	bone					1558:1561	the nature bone	1547:1561	the nature bone (1-200 MPa)	1547:1573	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	11	6	theme	nature	1551:1556	arg1	1-200 MPa					1564:1572	1-200 MPa	1564:1572	1-200 MPa	1564:1572	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	9	7	theme	good	1249:1252	arg1	capacity					1263:1270	a good swelling capacity	1247:1270	a good swelling capacity for the scaffolds	1247:1288	The results showed a good swelling capacity for the scaffolds, keeping their original shapes after swelling.
29673955	9	8	theme	original	1305:1312	arg1	shapes					1314:1319	their original shapes	1299:1319	their original shapes	1299:1319	The results showed a good swelling capacity for the scaffolds, keeping their original shapes after swelling.
29673955	4	9	theme	pressing	604:611	arg1	technique					613:621	cold isostatic pressing technique	589:621	cold isostatic pressing technique	589:621	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	13	10	theme	necessary	1804:1812	arg1	properties					1814:1823	necessary properties	1804:1823	necessary properties	1804:1823	The work might provide an efficient and alternative approach for collagen-based biomaterials with necessary properties.
29673955	5	11	theme	three-dimensional	683:699	arg1	structure					711:719	a three-dimensional bone-like structure	681:719	a three-dimensional bone-like structure	681:719	During the forming process, a three-dimensional bone-like structure was shaped in hybrid scaffolds.
29673955	10	12	theme	MFC	1432:1434	arg1	content					1436:1442	the MFC content	1428:1442	the MFC content	1428:1442	The compression strength and degradability of the scaffold materials could be regulated by the MFC content.
29673955	2	13	theme	in-situ	286:292	arg1	method					294:299	in-situ method	286:299	in-situ method	286:299	COL-HA was prepared by in-situ method and modified by dehydrothermal treatment.
29673955	6	14	dep	Fourier	827:833	arg1	transform					835:843	transform	835:843	transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD)	835:945	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	4	15	theme	isostatic	594:602	arg1	technique					613:621	cold isostatic pressing technique	589:621	cold isostatic pressing technique	589:621	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	13	16	theme	collagen-based	1771:1784	arg1	biomaterials					1786:1797	collagen-based biomaterials	1771:1797	collagen-based biomaterials with necessary properties	1771:1823	The work might provide an efficient and alternative approach for collagen-based biomaterials with necessary properties.
29673955	11	17	theme	scaffords	1494:1502	arg1	strength					1461:1468	The compression strength	1445:1468	The compression strength of COL-HA-MFC composite scaffords	1445:1502	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	14	18	theme	composite	1841:1849	arg1	scaffold					1851:1858	The COL-HA-MFC composite scaffold	1826:1858	The COL-HA-MFC composite scaffold	1826:1858	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	1	19	theme	microfibrillated	181:196	arg1	MFC					209:211	MFC	209:211	MFC	209:211	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	19	theme	microfibrillated	181:196	arg1	cellulose					198:206	microfibrillated cellulose	181:206	microfibrillated cellulose (MFC)	181:212	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	11	20	theme	compression	1449:1459	arg1	strength					1461:1468	The compression strength	1445:1468	The compression strength of COL-HA-MFC composite scaffords	1445:1502	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	0	21	theme	collagen-hydroxyapatite	49:71	arg1	scaffold					73:80	collagen-hydroxyapatite scaffold	49:80	collagen-hydroxyapatite scaffold for bone tissue engineering	49:108	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering.
29673955	11	22	theme	COL-HA-MFC	1473:1482	arg1	scaffords					1494:1502	COL-HA-MFC composite scaffords	1473:1502	COL-HA-MFC composite scaffords	1473:1502	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	6	23	theme	HA	969:970	arg1	deposited					972:980	HA deposited	969:980	HA deposited on collagen molecules	969:1002	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	12	24	from	biocompatibility	1611:1626	arg1	good					1603:1606	good	1603:1606	good	1603:1606	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	24	from	biocompatibility	1611:1626	arg1	scaffolds					1589:1597	The obtained scaffolds	1576:1597	The obtained scaffolds	1576:1597	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	6	25	theme	X-ray	923:927	arg1	XRD					942:944	XRD	942:944	XRD	942:944	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	6	25	theme	X-ray	923:927	arg1	diffraction					929:939	X-ray diffraction	923:939	X-ray diffraction (XRD)	923:945	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	7	26	theme	composites	1120:1129	arg1	property					1078:1085	mechanical property	1067:1085	mechanical property	1067:1085	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	7	26	theme	composites	1120:1129	arg1	degradability					1092:1104	degradability	1092:1104	degradability	1092:1104	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	7	26	theme	composites	1120:1129	arg1	Hydrophilicity					1032:1045	Hydrophilicity	1032:1045	Hydrophilicity	1032:1045	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	7	26	theme	composites	1120:1129	arg1	property					1057:1064	swelling property	1048:1064	swelling property	1048:1064	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	3	27	theme	Microfibrillated	343:358	arg1	polysaccharide					386:399	a nature polysaccharide	377:399	a nature polysaccharide with plenty of hydroxyl groups	377:430	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	3	27	theme	Microfibrillated	343:358	arg1	MFC					371:373	MFC	371:373	MFC	371:373	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	3	27	theme	Microfibrillated	343:358	arg1	cellulose					360:368	Microfibrillated cellulose	343:368	Microfibrillated cellulose (MFC)	343:374	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	2	28	theme	dehydrothermal	317:330	arg1	treatment					332:340	dehydrothermal treatment	317:340	dehydrothermal treatment	317:340	COL-HA was prepared by in-situ method and modified by dehydrothermal treatment.
29673955	6	29	dep	transform	835:843	arg1	infrared					845:852	infrared	845:852	transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD)	835:945	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	4	30	theme	MFC	566:568	arg1	ratios					545:550	different ratios	535:550	different ratios of COL-HA and MFC	535:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	1	31	with	composite	130:138	arg1	MFC					209:211	MFC	209:211	MFC	209:211	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	31	with	composite	130:138	arg1	cellulose					198:206	microfibrillated cellulose	181:206	microfibrillated cellulose (MFC)	181:212	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	9	32	theme	swelling	1254:1261	arg1	capacity					1263:1270	a good swelling capacity	1247:1270	a good swelling capacity for the scaffolds	1247:1288	The results showed a good swelling capacity for the scaffolds, keeping their original shapes after swelling.
29673955	12	33	theme	rate	1694:1697	arg1	level					1638:1642	high level	1633:1642	high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%)	1633:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	34	theme	suitable	1675:1682	arg1	%					1702:1702	≦5%	1700:1702	≦5%	1700:1702	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	34	theme	suitable	1675:1682	arg1	rate					1694:1697	suitable hemolysis rate	1675:1697	suitable hemolysis rate (≦5%)	1675:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	35	from	good	1603:1606	arg1	biocompatibility					1611:1626	biocompatibility	1611:1626	biocompatibility	1611:1626	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	0	36	theme	cellulose	34:42	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering	0:108	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering.
29673955	14	37	theme	COL-HA-MFC	1830:1839	arg1	scaffold					1851:1858	The COL-HA-MFC composite scaffold	1826:1858	The COL-HA-MFC composite scaffold	1826:1858	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	12	38	theme	growth	1652:1657	arg1	%					1668:1668	>70%	1665:1668	>70%	1665:1668	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	38	theme	growth	1652:1657	arg1	rate					1659:1662	cell growth rate	1647:1662	cell growth rate (>70%)	1647:1669	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	3	39	with	polysaccharide	386:399	arg1	plenty					406:411	plenty	406:411	plenty of hydroxyl groups	406:430	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	13	40	theme	alternative	1746:1756	arg1	approach					1758:1765	an efficient and alternative approach	1729:1765	an efficient and alternative approach for collagen-based biomaterials with necessary properties	1729:1823	The work might provide an efficient and alternative approach for collagen-based biomaterials with necessary properties.
29673955	3	41	theme	nature	379:384	arg1	polysaccharide					386:399	a nature polysaccharide	377:399	a nature polysaccharide with plenty of hydroxyl groups	377:430	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	3	41	theme	nature	379:384	arg1	cellulose					360:368	Microfibrillated cellulose	343:368	Microfibrillated cellulose (MFC)	343:374	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	12	42	theme	rate	1659:1662	arg1	level					1638:1642	high level	1633:1642	high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%)	1633:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	5	43	theme	hybrid	735:740	arg1	scaffolds					742:750	hybrid scaffolds	735:750	hybrid scaffolds	735:750	During the forming process, a three-dimensional bone-like structure was shaped in hybrid scaffolds.
29673955	11	44	theme	composite	1484:1492	arg1	scaffords					1494:1502	COL-HA-MFC composite scaffords	1473:1502	COL-HA-MFC composite scaffords	1473:1502	The compression strength of COL-HA-MFC composite scaffords increased to 20-40 MPa, closing to that of the nature bone (1-200 MPa).
29673955	3	45	theme	hydroxyl	416:423	arg1	groups					425:430	hydroxyl groups	416:430	hydroxyl groups	416:430	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	6	46	theme	collagen	985:992	arg1	molecules					994:1002	collagen molecules	985:1002	collagen molecules	985:1002	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	6	47	from	MFC	1008:1010	arg1	molecules					994:1002	collagen molecules	985:1002	collagen molecules	985:1002	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	10	48	theme	compression	1341:1351	arg1	strength					1353:1360	compression strength	1341:1360	compression strength	1341:1360	The compression strength and degradability of the scaffold materials could be regulated by the MFC content.
29673955	3	49	theme	COL-HA	455:460	arg1	composites					462:471	COL-HA composites	455:471	COL-HA composites	455:471	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	3	50	theme	groups	425:430	arg1	plenty					406:411	plenty	406:411	plenty of hydroxyl groups	406:430	Microfibrillated cellulose (MFC), a nature polysaccharide with plenty of hydroxyl groups, was incorporated into COL-HA composites to improve the properties.
29673955	12	51	with	good	1603:1606	arg1	level					1638:1642	high level	1633:1642	high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%)	1633:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	7	52	theme	swelling	1048:1055	arg1	property					1057:1064	swelling property	1048:1064	swelling property	1048:1064	Hydrophilicity, swelling property, mechanical property, and degradability of COL-HA-MFC composites were investigated.
29673955	13	53	theme	efficient	1732:1740	arg1	approach					1758:1765	an efficient and alternative approach	1729:1765	an efficient and alternative approach for collagen-based biomaterials with necessary properties	1729:1823	The work might provide an efficient and alternative approach for collagen-based biomaterials with necessary properties.
29673955	4	54	theme	different	535:543	arg1	ratios					545:550	different ratios	535:550	different ratios of COL-HA and MFC	535:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	5	55	theme	bone-like	701:709	arg1	structure					711:719	a three-dimensional bone-like structure	681:719	a three-dimensional bone-like structure	681:719	During the forming process, a three-dimensional bone-like structure was shaped in hybrid scaffolds.
29673955	14	56	theme	bone	1894:1897	arg1	engineering					1906:1916	bone tissue engineering	1894:1916	bone tissue engineering	1894:1916	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	13	57	with	biomaterials	1786:1797	arg1	properties					1814:1823	necessary properties	1804:1823	necessary properties	1804:1823	The work might provide an efficient and alternative approach for collagen-based biomaterials with necessary properties.
29673955	10	58	theme	scaffold	1387:1394	arg1	materials					1396:1404	the scaffold materials	1383:1404	the scaffold materials	1383:1404	The compression strength and degradability of the scaffold materials could be regulated by the MFC content.
29673955	12	59	theme	obtained	1580:1587	arg1	good					1603:1606	good	1603:1606	good	1603:1606	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	59	theme	obtained	1580:1587	arg1	scaffolds					1589:1597	The obtained scaffolds	1576:1597	The obtained scaffolds	1576:1597	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	4	60	theme	COL-HA	555:560	arg1	ratios					545:550	different ratios	535:550	different ratios of COL-HA and MFC	535:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	8	61	theme	Biological	1150:1159	arg1	properties					1161:1170	Biological properties	1150:1170	Biological properties	1150:1170	Biological properties, such as cytotoxicity and hemolysis, were also studied.
29673955	8	61	theme	Biological	1150:1159	arg1	hemolysis					1198:1206	hemolysis	1198:1206	hemolysis	1198:1206	Biological properties, such as cytotoxicity and hemolysis, were also studied.
29673955	8	61	theme	Biological	1150:1159	arg1	cytotoxicity					1181:1192	cytotoxicity	1181:1192	cytotoxicity	1181:1192	Biological properties, such as cytotoxicity and hemolysis, were also studied.
29673955	6	62	theme	composites	799:808	arg1	transitions					773:783	The microstructural transitions	753:783	The microstructural transitions of COL-HA-MFC composites	753:808	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	4	63	with	scaffold	521:528	arg1	ratios					545:550	different ratios	535:550	different ratios of COL-HA and MFC	535:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	12	64	theme	high	1633:1636	arg1	level					1638:1642	high level	1633:1642	high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%)	1633:1703	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	14	65	theme	tissue	1899:1904	arg1	engineering					1906:1916	bone tissue engineering	1894:1916	bone tissue engineering	1894:1916	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	6	66	theme	COL-HA-MFC	788:797	arg1	composites					799:808	COL-HA-MFC composites	788:808	COL-HA-MFC composites	788:808	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	14	67	from	application	1879:1889	arg1	engineering					1906:1916	bone tissue engineering	1894:1916	bone tissue engineering	1894:1916	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	6	68	from	deposited	972:980	arg1	molecules					994:1002	collagen molecules	985:1002	collagen molecules	985:1002	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	1	69	dep	material	253:260	arg1	substitute					242:251	substitute	242:251	substitute	242:251	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	10	70	theme	materials	1396:1404	arg1	strength					1353:1360	compression strength	1341:1360	compression strength	1341:1360	The compression strength and degradability of the scaffold materials could be regulated by the MFC content.
29673955	10	70	theme	materials	1396:1404	arg1	degradability					1366:1378	degradability	1366:1378	degradability	1366:1378	The compression strength and degradability of the scaffold materials could be regulated by the MFC content.
29673955	1	71	theme	Collagen-Hydroxyapitite	143:165	arg1	Collagen-Hydroxyapitite					143:165	Collagen-Hydroxyapitite	143:165	Collagen-Hydroxyapitite (COL-HA)	143:174	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	71	theme	Collagen-Hydroxyapitite	143:165	arg1	composite					130:138	the composite	126:138	the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC)	126:212	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	71	theme	Collagen-Hydroxyapitite	143:165	arg1	material					253:260	a new bone substitute material	231:260	a new bone substitute material	231:260	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	72	theme	new	233:235	arg1	Collagen-Hydroxyapitite					143:165	Collagen-Hydroxyapitite	143:165	Collagen-Hydroxyapitite (COL-HA)	143:174	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	72	theme	new	233:235	arg1	composite					130:138	the composite	126:138	the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC)	126:212	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	72	theme	new	233:235	arg1	material					253:260	a new bone substitute material	231:260	a new bone substitute material	231:260	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	0	73	theme	tissue	91:96	arg1	engineering					98:108	bone tissue engineering	86:108	bone tissue engineering	86:108	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering.
29673955	12	74	theme	cell	1647:1650	arg1	%					1668:1668	>70%	1665:1668	>70%	1665:1668	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	12	74	theme	cell	1647:1650	arg1	rate					1659:1662	cell growth rate	1647:1662	cell growth rate (>70%)	1647:1669	The obtained scaffolds are good in biocompatibility with high level of cell growth rate (>70%) and suitable hemolysis rate (≦5%).
29673955	14	75	theme	potential	1869:1877	arg1	application					1879:1889	a potential application	1867:1889	a potential application in bone tissue engineering	1867:1916	The COL-HA-MFC composite scaffold showed a potential application in bone tissue engineering.
29673955	1	76	theme	bone	237:240	arg1	Collagen-Hydroxyapitite					143:165	Collagen-Hydroxyapitite	143:165	Collagen-Hydroxyapitite (COL-HA)	143:174	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	76	theme	bone	237:240	arg1	composite					130:138	the composite	126:138	the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC)	126:212	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	1	76	theme	bone	237:240	arg1	material					253:260	a new bone substitute material	231:260	a new bone substitute material	231:260	In this study, the composite of Collagen-Hydroxyapitite (COL-HA) with microfibrillated cellulose (MFC) was developed as a new bone substitute material.
29673955	0	77	theme	bone	86:89	arg1	engineering					98:108	bone tissue engineering	86:108	bone tissue engineering	86:108	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering.
29673955	4	78	theme	COL-HA-MFC	510:519	arg1	scaffold					521:528	The novel COL-HA-MFC scaffold	500:528	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC	500:568	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
29673955	0	79	theme	microfibrillated	17:32	arg1	cellulose					34:42	microfibrillated cellulose	17:42	microfibrillated cellulose	17:42	Incorporation of microfibrillated cellulose into collagen-hydroxyapatite scaffold for bone tissue engineering.
29673955	6	80	theme	microstructural	757:771	arg1	transitions					773:783	The microstructural transitions	753:783	The microstructural transitions of COL-HA-MFC composites	753:808	The microstructural transitions of COL-HA-MFC composites were examined by Fourier transform infrared spectroscope (FTIR), Ultraviolet-visible spectrophotometer (UV), and X-ray diffraction (XRD), which indicated that HA deposited on collagen molecules and MFC bonded with COL-HA.
29673955	5	81	theme	forming	664:670	arg1	process					672:678	the forming process	660:678	the forming process	660:678	During the forming process, a three-dimensional bone-like structure was shaped in hybrid scaffolds.
29673955	4	82	theme	freeze-drying	627:639	arg1	technology					641:650	freeze-drying technology	627:650	freeze-drying technology	627:650	The novel COL-HA-MFC scaffold with different ratios of COL-HA and MFC were fabricated by cold isostatic pressing technique and freeze-drying technology.
30451067	4	0	theme	hydrothermal	615:626	arg1	synthesis					628:636	hydrothermal synthesis	615:636	hydrothermal synthesis of cuttlebone	615:650	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	3	1	theme	engineering	532:542	arg1	applications					544:555	tissue engineering applications	525:555	tissue engineering applications	525:555	Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications.
30451067	1	2	theme	wide	288:291	arg1	accessibility					293:305	wide accessibility	288:305	wide accessibility	288:305	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	2	3	theme	marine	320:325	arg1	sources					327:333	these marine sources	314:333	these marine sources	314:333	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	5	4	from	analysis	776:783	arg1	zones					835:839	different coastal zones	817:839	different coastal zones	817:839	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	4	from	analysis	776:783	arg1	HAp					864:866	cuttlebone-derived HAp	845:866	cuttlebone-derived HAp	845:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	1	5	theme	marine-derived	130:143	arg1	materials					145:153	marine-derived materials	130:153	marine-derived materials	130:153	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	4	6	theme	osteoconductive	708:722	arg1	scaffold					734:741	an osteoconductive composite scaffold	705:741	an osteoconductive composite scaffold for bone regeneration	705:763	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	10	7	link	cuttlebone-derived	1579:1596	arg1	incorporation					1602:1614	cuttlebone-derived HAp incorporation	1579:1614	cuttlebone-derived HAp incorporation	1579:1614	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	10	8	theme	compression	1558:1568	arg1	test					1570:1573	a compression test	1556:1573	a compression test	1556:1573	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	13	9	theme	bone	1969:1972	arg1	regeneration					1981:1992	bone tissue regeneration	1969:1992	bone tissue regeneration	1969:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	5	10	link	cuttlebone-derived	845:862	arg1	HAp					864:866	cuttlebone-derived HAp	845:866	cuttlebone-derived HAp	845:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	9	11	theme	mean	1438:1441	arg1	size					1448:1451	The mean pore size	1434:1451	The mean pore size of the scaffolds	1434:1468	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	9	11	theme	mean	1438:1441	arg1	µm					1478:1479	510 µm	1474:1479	510 µm	1474:1479	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	0	12	from	hydroxyapatite	95:108	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	10	13	theme	HAp	1598:1600	arg1	incorporation					1602:1614	cuttlebone-derived HAp incorporation	1579:1614	cuttlebone-derived HAp incorporation	1579:1614	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	5	14	theme	raw	788:790	arg1	material					803:810	raw cuttlebone material	788:810	raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	788:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	1	15	theme	materials	145:153	arg1	usage					121:125	usage	121:125	usage of marine-derived materials in biomedical field	121:173	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	7	16	theme	composite	1313:1321	arg1	scaffolds					1323:1331	cellulose-based composite scaffolds	1297:1331	cellulose-based composite scaffolds	1297:1331	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	1	17	theme	biomedical	158:167	arg1	field					169:173	biomedical field	158:173	biomedical field	158:173	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	1	18	theme	promising	213:221	arg1	characteristics					223:237	their promising characteristics	207:237	their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility	207:305	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	1	18	theme	promising	213:221	arg1	biocompatibility					247:262	biocompatibility	247:262	biocompatibility	247:262	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	1	18	theme	promising	213:221	arg1	immunogenicity					269:282	low immunogenicity	265:282	low immunogenicity	265:282	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	1	18	theme	promising	213:221	arg1	accessibility					293:305	wide accessibility	288:305	wide accessibility	288:305	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	4	19	theme	bone	747:750	arg1	regeneration					752:763	bone regeneration	747:763	bone regeneration	747:763	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	13	20	theme	major	1954:1958	arg1	step					1960:1963	the major step	1950:1963	the major step for bone tissue regeneration	1950:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	13	20	theme	major	1954:1958	arg1	mineralization					1926:1939	the surface mineralization	1914:1939	the surface mineralization which is the major step for bone tissue regeneration	1914:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	5	21	theme	cuttlebone-derived	845:862	arg1	HAp					864:866	cuttlebone-derived HAp	845:866	cuttlebone-derived HAp	845:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	22	from	HAp	864:866	arg1	analysis					776:783	Elemental analysis	766:783	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	766:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	22	from	HAp	864:866	arg1	material					803:810	raw cuttlebone material	788:810	raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	788:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	23	theme	material	803:810	arg1	analysis					776:783	Elemental analysis	766:783	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	766:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	9	24	theme	%	1502:1502	arg1	porosity					1488:1495	a porosity	1486:1495	a porosity of 85%	1486:1502	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	2	25	theme	traditional	425:435	arg1	medicine					437:444	traditional medicine	425:444	traditional medicine	425:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	5	26	theme	different	817:825	arg1	zones					835:839	different coastal zones	817:839	different coastal zones	817:839	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	6	27	theme	heavy	1043:1047	arg1	Cd					1069:1070	Cd	1069:1070	Cd	1069:1070	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	27	theme	heavy	1043:1047	arg1	Pb					1073:1074	Pb	1073:1074	Pb	1073:1074	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	27	theme	heavy	1043:1047	arg1	Ag					1065:1066	Ag	1065:1066	Ag	1065:1066	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	27	theme	heavy	1043:1047	arg1	V					1079:1079	V	1079:1079	V	1079:1079	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	27	theme	heavy	1043:1047	arg1	metals					1049:1054	biologically unfavorable heavy metals	1018:1054	biologically unfavorable heavy metals	1018:1054	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	0	28	theme	cuttlebone-derived	76:93	arg1	hydroxyapatite					95:108	cuttlebone-derived hydroxyapatite	76:108	cuttlebone-derived hydroxyapatite	76:108	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	8	29	theme	Interconnected	1334:1347	arg1	structure					1363:1371	Interconnected highly porous structure	1334:1371	Interconnected highly porous structure of the scaffolds	1334:1388	Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography.
30451067	0	30	theme	Osteoconductive	0:14	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	4	31	theme	hydroxyapatite	573:586	arg1	particles					588:596	hydroxyapatite particles	573:596	hydroxyapatite particles	573:596	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	8	32	theme	porous	1356:1361	arg1	structure					1363:1371	Interconnected highly porous structure	1334:1371	Interconnected highly porous structure of the scaffolds	1334:1388	Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography.
30451067	3	33	theme	cuttlebone	489:498	arg1	use					482:484	the use	478:484	the use of cuttlebone as a bioactive agent for tissue engineering applications	478:555	Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications.
30451067	0	34	theme	porous	19:24	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	7	35	theme	mineral	1259:1265	arg1	particle					1154:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	7	35	theme	mineral	1259:1265	arg1	filler					1267:1272	a mineral filler	1257:1272	a mineral filler	1257:1272	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	3	36	theme	Recent	447:452	arg1	studies					454:460	Recent studies	447:460	Recent studies	447:460	Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications.
30451067	11	37	theme	culture	1689:1695	arg1	studies					1697:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds.
30451067	10	38	theme	cellulose	1654:1662	arg1	scaffolds					1664:1672	cellulose scaffolds	1654:1672	cellulose scaffolds	1654:1672	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	2	39	from	composition	410:420	arg1	medicine					437:444	traditional medicine	425:444	traditional medicine	425:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	8	40	theme	scaffolds	1380:1388	arg1	structure					1363:1371	Interconnected highly porous structure	1334:1371	Interconnected highly porous structure of the scaffolds	1334:1388	Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography.
30451067	2	41	theme	valuable	366:373	arg1	cuttlebone					336:345	cuttlebone	336:345	cuttlebone	336:345	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	2	41	theme	valuable	366:373	arg1	component					375:383	a valuable component	364:383	a valuable component with its trace elemental composition in traditional medicine	364:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	5	42	theme	various	880:886	arg1	macro-					888:893	various macro-	880:893	various macro-	880:893	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	43	theme	similar	992:998	arg1	amount					1000:1005	a very similar amount	985:1005	a very similar amount	985:1005	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	0	44	link	cuttlebone-derived	76:93	arg1	hydroxyapatite					95:108	cuttlebone-derived hydroxyapatite	76:108	cuttlebone-derived hydroxyapatite	76:108	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	7	45	theme	cellulose-based	1297:1311	arg1	scaffolds					1323:1331	cellulose-based composite scaffolds	1297:1331	cellulose-based composite scaffolds	1297:1331	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	1	46	link	marine-derived	130:143	arg1	materials					145:153	marine-derived materials	130:153	marine-derived materials	130:153	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	11	47	theme	MG-63	1720:1724	arg1	cells					1726:1730	MG-63 cells	1720:1730	MG-63 cells	1720:1730	In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds.
30451067	11	48	dep	In	1675:1676	arg1	vitro					1678:1682	vitro	1678:1682	vitro	1678:1682	In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds.
30451067	12	49	theme	cuttlebone-derived	1777:1794	arg1	hydroxyapatite					1796:1809	cuttlebone-derived hydroxyapatite	1777:1809	cuttlebone-derived hydroxyapatite	1777:1809	In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion.
30451067	7	50	theme	hydrothermal	1222:1233	arg1	treatment					1235:1243	hydrothermal treatment	1222:1243	hydrothermal treatment	1222:1243	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	4	51	theme	cuttlebone	641:650	arg1	synthesis					628:636	hydrothermal synthesis	615:636	hydrothermal synthesis of cuttlebone	615:650	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	8	52	theme	micro-computed	1407:1420	arg1	tomography					1422:1431	micro-computed tomography	1407:1431	micro-computed tomography	1407:1431	Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography.
30451067	2	53	theme	trace	394:398	arg1	composition					410:420	its trace elemental composition	390:420	its trace elemental composition in traditional medicine	390:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	1	54	theme	low	265:267	arg1	immunogenicity					269:282	low immunogenicity	265:282	low immunogenicity	265:282	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	3	55	theme	tissue	525:530	arg1	applications					544:555	tissue engineering applications	525:555	tissue engineering applications	525:555	Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications.
30451067	5	56	located	found	976:980	arg1	amount					1000:1005	a very similar amount	985:1005	a very similar amount	985:1005	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	micro-					896:901	micro-	896:901	micro-	896:901	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	P					928:928	P	928:928	P	928:928	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	Zn					968:969	Zn	968:969	Zn	968:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	Br					957:958	Br	957:958	Br	957:958	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	elements					913:920	trace elements	907:920	trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn	907:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	Fe					961:962	Fe	961:962	Fe	961:962	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	Ca					924:925	Ca	924:925	Ca	924:925	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	56	located	found	976:980	arg2	macro-					888:893	various macro-	880:893	various macro-	880:893	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	6	57	located	detected	1091:1098	arg2	Ag					1065:1066	Ag	1065:1066	Ag	1065:1066	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	57	located	detected	1091:1098	arg2	V					1079:1079	V	1079:1079	V	1079:1079	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	57	located	detected	1091:1098	arg2	metals					1049:1054	biologically unfavorable heavy metals	1018:1054	biologically unfavorable heavy metals	1018:1054	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	57	located	detected	1091:1098	arg2	Cd					1069:1070	Cd	1069:1070	Cd	1069:1070	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	57	located	detected	1091:1098	arg2	Pb					1073:1074	Pb	1073:1074	Pb	1073:1074	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	57	located	detected	1091:1098	arg1	specimen					1118:1125	any cuttlebone specimen	1103:1125	any cuttlebone specimen	1103:1125	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	0	58	theme	regenerated	50:60	arg1	cellulose					62:70	regenerated cellulose	50:70	regenerated cellulose	50:70	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	4	59	theme	composite	724:732	arg1	scaffold					734:741	an osteoconductive composite scaffold	705:741	an osteoconductive composite scaffold for bone regeneration	705:763	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	5	60	theme	Elemental	766:774	arg1	analysis					776:783	Elemental analysis	766:783	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	766:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	13	61	theme	tissue	1974:1979	arg1	regeneration					1981:1992	bone tissue regeneration	1969:1992	bone tissue regeneration	1969:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	5	62	theme	trace	907:911	arg1	elements					913:920	trace elements	907:920	trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn	907:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	62	theme	trace	907:911	arg1	Br					957:958	Br	957:958	Br	957:958	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	62	theme	trace	907:911	arg1	Ca					924:925	Ca	924:925	Ca	924:925	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	62	theme	trace	907:911	arg1	Fe					961:962	Fe	961:962	Fe	961:962	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	62	theme	trace	907:911	arg1	P					928:928	P	928:928	P	928:928	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	62	theme	trace	907:911	arg1	Zn					968:969	Zn	968:969	Zn	968:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	4	63	theme	cellulose	672:680	arg1	scaffolds					682:690	cellulose scaffolds	672:690	cellulose scaffolds	672:690	In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration.
30451067	10	64	theme	cuttlebone-derived	1579:1596	arg1	incorporation					1602:1614	cuttlebone-derived HAp incorporation	1579:1614	cuttlebone-derived HAp incorporation	1579:1614	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	9	65	theme	scaffolds	1460:1468	arg1	size					1448:1451	The mean pore size	1434:1451	The mean pore size of the scaffolds	1434:1468	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	9	65	theme	scaffolds	1460:1468	arg1	µm					1478:1479	510 µm	1474:1479	510 µm	1474:1479	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	7	66	theme	scaffolds	1323:1331	arg1	preparation					1282:1292	the preparation	1278:1292	the preparation of cellulose-based composite scaffolds	1278:1331	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	5	67	dep	Br	957:958	arg1	Sr					943:944	Na, Mg, Cu, Sr, Cl, K, S, Br	931:958	Sr	943:944	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	67	dep	Br	957:958	arg1	Cl					947:948	Na, Mg, Cu, Sr, Cl, K, S, Br	931:958	Cl	947:948	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	67	dep	Br	957:958	arg1	Mg					935:936	Na, Mg, Cu, Sr, Cl, K, S, Br	931:958	Mg	935:936	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	67	dep	Br	957:958	arg1	Cu					939:940	Na, Mg, Cu, Sr, Cl, K, S, Br	931:958	Cu	939:940	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	67	dep	Br	957:958	arg1	K					951:951	Na, Mg, Cu, Sr, Cl, K, S, Br	931:958	K	951:951	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	67	dep	Br	957:958	arg1	S					954:954	S	954:954	S	954:954	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	68	theme	coastal	827:833	arg1	zones					835:839	different coastal zones	817:839	different coastal zones	817:839	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	69	from	zones	835:839	arg1	analysis					776:783	Elemental analysis	766:783	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	766:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	69	from	zones	835:839	arg1	material					803:810	raw cuttlebone material	788:810	raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	788:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	9	70	theme	pore	1443:1446	arg1	size					1448:1451	The mean pore size	1434:1451	The mean pore size of the scaffolds	1434:1468	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	9	70	theme	pore	1443:1446	arg1	µm					1478:1479	510 µm	1474:1479	510 µm	1474:1479	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	6	71	theme	cuttlebone	1107:1116	arg1	specimen					1118:1125	any cuttlebone specimen	1103:1125	any cuttlebone specimen	1103:1125	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	7	72	theme	Carbonated	1128:1137	arg1	particle					1154:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	7	72	theme	Carbonated	1128:1137	arg1	filler					1267:1272	a mineral filler	1257:1272	a mineral filler	1257:1272	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	7	73	theme	hydroxyapatite	1139:1152	arg1	particle					1154:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	7	73	theme	hydroxyapatite	1139:1152	arg1	filler					1267:1272	a mineral filler	1257:1272	a mineral filler	1257:1272	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	13	74	theme	surface	1918:1924	arg1	step					1960:1963	the major step	1950:1963	the major step for bone tissue regeneration	1950:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	13	74	theme	surface	1918:1924	arg1	mineralization					1926:1939	the surface mineralization	1914:1939	the surface mineralization which is the major step for bone tissue regeneration	1914:1992	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	5	75	theme	cuttlebone	792:801	arg1	material					803:810	raw cuttlebone material	788:810	raw cuttlebone material from different coastal zones and cuttlebone-derived HAp	788:866	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	0	76	from	cellulose	62:70	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	9	77	with	µm	1478:1479	arg1	porosity					1488:1495	a porosity	1486:1495	a porosity of 85%	1486:1502	The mean pore size of the scaffolds was 510 µm with a porosity of 85%.
30451067	2	78	used	used	356:359	arg2	cuttlebone					336:345	cuttlebone	336:345	cuttlebone	336:345	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	2	78	used	used	356:359	arg2	component					375:383	a valuable component	364:383	a valuable component with its trace elemental composition in traditional medicine	364:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	13	79	theme	HAp	1886:1888	arg1	incorporation					1890:1902	HAp incorporation	1886:1902	HAp incorporation	1886:1902	Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.
30451067	10	80	theme	mechanical	1629:1638	arg1	properties					1640:1649	the mechanical properties	1625:1649	the mechanical properties of cellulose scaffolds	1625:1672	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	0	81	theme	3D	16:17	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	6	82	theme	unfavorable	1031:1041	arg1	Cd					1069:1070	Cd	1069:1070	Cd	1069:1070	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	82	theme	unfavorable	1031:1041	arg1	Pb					1073:1074	Pb	1073:1074	Pb	1073:1074	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	82	theme	unfavorable	1031:1041	arg1	Ag					1065:1066	Ag	1065:1066	Ag	1065:1066	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	82	theme	unfavorable	1031:1041	arg1	V					1079:1079	V	1079:1079	V	1079:1079	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	6	82	theme	unfavorable	1031:1041	arg1	metals					1049:1054	biologically unfavorable heavy metals	1018:1054	biologically unfavorable heavy metals	1018:1054	Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen.
30451067	7	83	used	used	1249:1252	arg2	particle					1154:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle	1128:1161	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	7	83	used	used	1249:1252	arg2	filler					1267:1272	a mineral filler	1257:1272	a mineral filler	1257:1272	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	2	84	theme	elemental	400:408	arg1	composition					410:420	its trace elemental composition	390:420	its trace elemental composition in traditional medicine	390:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	0	85	theme	composite	26:34	arg1	scaffold					36:43	Osteoconductive 3D porous composite scaffold	0:43	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.	0:109	Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite.
30451067	11	86	theme	cell	1684:1687	arg1	studies					1697:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds.
30451067	10	87	theme	scaffolds	1664:1672	arg1	properties					1640:1649	the mechanical properties	1625:1649	the mechanical properties of cellulose scaffolds	1625:1672	The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds.
30451067	11	88	theme	In	1675:1676	arg1	studies					1697:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies	1675:1703	In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds.
30451067	12	89	link	cuttlebone-derived	1777:1794	arg1	hydroxyapatite					1796:1809	cuttlebone-derived hydroxyapatite	1777:1809	cuttlebone-derived hydroxyapatite	1777:1809	In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion.
30451067	12	90	theme	osteocalcin	1854:1864	arg1	secretion					1866:1874	osteocalcin secretion	1854:1874	osteocalcin secretion	1854:1874	In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion.
30451067	1	91	from	usage	121:125	arg1	field					169:173	biomedical field	158:173	biomedical field	158:173	Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility.
30451067	2	92	with	component	375:383	arg1	composition					410:420	its trace elemental composition	390:420	its trace elemental composition in traditional medicine	390:444	Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine.
30451067	12	93	theme	ALP	1837:1839	arg1	activity					1841:1848	the ALP activity	1833:1848	the ALP activity	1833:1848	In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion.
30451067	3	94	theme	bioactive	505:513	arg1	agent					515:519	a bioactive agent	503:519	a bioactive agent for tissue engineering applications	503:555	Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications.
30451067	7	95	theme	cuttlebone	1192:1201	arg1	microparticles					1203:1216	cuttlebone microparticles	1192:1216	cuttlebone microparticles	1192:1216	Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds.
30451067	5	96	dep	elements	913:920	arg1	elements					913:920	trace elements	907:920	trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn	907:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	96	dep	elements	913:920	arg1	Br					957:958	Br	957:958	Br	957:958	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	96	dep	elements	913:920	arg1	Ca					924:925	Ca	924:925	Ca	924:925	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	96	dep	elements	913:920	arg1	Fe					961:962	Fe	961:962	Fe	961:962	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	96	dep	elements	913:920	arg1	P					928:928	P	928:928	P	928:928	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
30451067	5	96	dep	elements	913:920	arg1	Zn					968:969	Zn	968:969	Zn	968:969	Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount.
31100395	3	0	theme	antimicrobial	688:700	arg1	properties					702:711	in vitro antimicrobial properties	679:711	in vitro antimicrobial properties	679:711	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	4	1	theme	water	853:857	arg1	solubility					859:868	water solubility	853:868	water solubility	853:868	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	1	2	from	effect	172:177	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	1	3	theme	present	151:157	arg1	research					159:166	the present research	147:166	the present research	147:166	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	3	4	theme	Listeria	721:728	arg1	monocytogenes					730:742	Listeria monocytogenes	721:742	Listeria monocytogenes	721:742	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	5	theme	acid	232:235	arg1	effect					172:177	the effect	168:177	the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	168:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	2	6	theme	FTIR	463:466	arg1	analysis					468:475	FTIR analysis	463:475	FTIR analysis	463:475	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	1	7	theme	mechanical	297:306	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	8	theme	cinnamon	105:112	arg1	oil					124:126	cinnamon essential oil	105:126	cinnamon essential oil	105:126	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	0	9	from	Development	0:10	arg1	chitosan					46:53	chitosan	46:53	chitosan	46:53	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	0	9	from	Development	0:10	arg1	cellulose					73:81	carboxymethyl cellulose	59:81	carboxymethyl cellulose	59:81	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	4	10	from	break	797:801	arg1	elongation					783:792	elongation at break	783:801	elongation at break (EAB)	783:807	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	4	10	from	break	797:801	arg1	at					794:795	elongation at break	783:801	elongation at break (EAB)	783:807	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	2	11	theme	higher	504:509	arg1	strength					522:529	higher mechanical strength	504:529	higher mechanical strength	504:529	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	1	12	theme	physical	309:316	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	5	13	theme	Color	895:899	arg1	properties					901:910	Color properties	895:910	Color properties	895:910	Color properties and microstructure of composite films were significantly affected by the additives as confirmed by SEM analyses.
31100395	6	14	theme	composite	1143:1151	arg1	films					1153:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	0	15	from	chitosan	46:53	arg1	Development					0:10	Development	0:10	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.	0:142	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	0	15	from	chitosan	46:53	arg1	films					35:39	bioactive composite films	15:39	bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid	15:141	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	4	16	theme	at	794:795	arg1	EAB					804:806	EAB	804:806	EAB	804:806	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	4	16	theme	at	794:795	arg1	break					797:801	elongation at break	783:801	elongation at break (EAB)	783:807	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	6	17	theme	physical	1087:1094	arg1	properties					1096:1105	the antimicrobial, antioxidant, mechanical and physical properties	1040:1105	the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films	1040:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	0	18	theme	essential	114:122	arg1	oil					124:126	cinnamon essential oil	105:126	cinnamon essential oil	105:126	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	1	19	theme	morphological	322:334	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	4	20	theme	elongation	783:792	arg1	EAB					804:806	EAB	804:806	EAB	804:806	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	4	20	theme	elongation	783:792	arg1	break					797:801	elongation at break	783:801	elongation at break (EAB)	783:807	Thickness, elongation at break (EAB) and WVP increased by including OA; however, water solubility significantly decreased.
31100395	2	21	theme	GL	442:443	arg1	effect					432:437	Cross-linking effect	418:437	Cross-linking effect of GL (as determined by FTIR analysis)	418:476	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	2	22	dep	GL	442:443	arg1	determined					449:458	determined	449:458	determined by FTIR analysis	449:475	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	5	23	theme	films	944:948	arg1	microstructure					916:929	microstructure	916:929	microstructure of composite films	916:948	Color properties and microstructure of composite films were significantly affected by the additives as confirmed by SEM analyses.
31100395	5	23	theme	films	944:948	arg1	properties					901:910	Color properties	895:910	Color properties	895:910	Color properties and microstructure of composite films were significantly affected by the additives as confirmed by SEM analyses.
31100395	6	24	theme	mechanical	1072:1081	arg1	properties					1096:1105	the antimicrobial, antioxidant, mechanical and physical properties	1040:1105	the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films	1040:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	3	25	dep	in	679:680	arg1	vitro					682:686	vitro	682:686	vitro	682:686	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	26	theme	incorporating	182:194	arg1	CEO					220:222	CEO	220:222	CEO	220:222	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	1	26	theme	incorporating	182:194	arg1	oil					215:217	incorporating cinnamon essential oil	182:217	incorporating cinnamon essential oil (CEO)	182:223	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	27	theme	composite	25:33	arg1	films					35:39	bioactive composite films	15:39	bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid	15:141	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	1	28	theme	glutaraldehyde	246:259	arg1	effect					172:177	the effect	168:177	the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	168:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	29	theme	oleic	132:136	arg1	acid					138:141	oleic acid	132:141	oleic acid	132:141	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	6	30	theme	antimicrobial	1044:1056	arg1	properties					1096:1105	the antimicrobial, antioxidant, mechanical and physical properties	1040:1105	the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films	1040:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	1	31	theme	cinnamon	196:203	arg1	CEO					220:222	CEO	220:222	CEO	220:222	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	1	31	theme	cinnamon	196:203	arg1	oil					215:217	incorporating cinnamon essential oil	182:217	incorporating cinnamon essential oil (CEO)	182:223	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	32	theme	bioactive	15:23	arg1	films					35:39	bioactive composite films	15:39	bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid	15:141	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	2	33	theme	lower	535:539	arg1	permeability					553:564	lower water vapor permeability	535:564	lower water vapor permeability (WVP)	535:570	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	2	33	theme	lower	535:539	arg1	WVP					567:569	WVP	567:569	WVP	567:569	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	3	34	theme	steady	634:639	arg1	state					641:645	steady state	634:645	steady state antioxidant activity	634:666	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	35	theme	essential	205:213	arg1	CEO					220:222	CEO	220:222	CEO	220:222	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	1	35	theme	essential	205:213	arg1	oil					215:217	incorporating cinnamon essential oil	182:217	incorporating cinnamon essential oil (CEO)	182:223	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	3	36	theme	state	641:645	arg1	activity					659:666	steady state antioxidant activity	634:666	steady state antioxidant activity	634:666	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	37	theme	chitosan-carboxymethyl	350:371	arg1	films					393:397	chitosan-carboxymethyl cellulose composite films	350:397	chitosan-carboxymethyl cellulose composite films	350:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	3	38	theme	Cross-linked	573:584	arg1	films					586:590	Cross-linked films	573:590	Cross-linked films incorporated with CEO	573:612	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	39	theme	oil	215:217	arg1	effect					172:177	the effect	168:177	the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	168:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	40	theme	films	35:39	arg1	Development					0:10	Development	0:10	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.	0:142	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	1	41	theme	cellulose	373:381	arg1	films					393:397	chitosan-carboxymethyl cellulose composite films	350:397	chitosan-carboxymethyl cellulose composite films	350:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	3	42	link	Cross-linked	573:584	arg1	films					586:590	Cross-linked films	573:590	Cross-linked films incorporated with CEO	573:612	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	2	43	theme	mechanical	511:520	arg1	strength					522:529	higher mechanical strength	504:529	higher mechanical strength	504:529	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	1	44	theme	composite	383:391	arg1	films					393:397	chitosan-carboxymethyl cellulose composite films	350:397	chitosan-carboxymethyl cellulose composite films	350:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	3	45	theme	in	679:680	arg1	properties					702:711	in vitro antimicrobial properties	679:711	in vitro antimicrobial properties	679:711	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	1	46	theme	antimicrobial	269:281	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	2	47	theme	water	541:545	arg1	permeability					553:564	lower water vapor permeability	535:564	lower water vapor permeability (WVP)	535:570	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	2	47	theme	water	541:545	arg1	WVP					567:569	WVP	567:569	WVP	567:569	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	1	48	theme	films	393:397	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	0	49	from	cellulose	73:81	arg1	Development					0:10	Development	0:10	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.	0:142	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	0	49	from	cellulose	73:81	arg1	films					35:39	bioactive composite films	15:39	bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid	15:141	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	6	50	theme	film	1213:1216	arg1	matrix					1218:1223	the film matrix	1209:1223	the film matrix	1209:1223	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	6	51	theme	films	1153:1157	arg1	properties					1096:1105	the antimicrobial, antioxidant, mechanical and physical properties	1040:1105	the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films	1040:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	2	52	theme	vapor	547:551	arg1	permeability					553:564	lower water vapor permeability	535:564	lower water vapor permeability (WVP)	535:570	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	2	52	theme	vapor	547:551	arg1	WVP					567:569	WVP	567:569	WVP	567:569	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	3	53	theme	antioxidant	647:657	arg1	activity					659:666	steady state antioxidant activity	634:666	steady state antioxidant activity	634:666	Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomonas aeruginosa.
31100395	5	54	theme	composite	934:942	arg1	films					944:948	composite films	934:948	composite films	934:948	Color properties and microstructure of composite films were significantly affected by the additives as confirmed by SEM analyses.
31100395	5	55	theme	SEM	1011:1013	arg1	analyses					1015:1022	SEM analyses	1011:1022	SEM analyses	1011:1022	Color properties and microstructure of composite films were significantly affected by the additives as confirmed by SEM analyses.
31100395	6	56	theme	antioxidant	1059:1069	arg1	properties					1096:1105	the antimicrobial, antioxidant, mechanical and physical properties	1040:1105	the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films	1040:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	6	57	theme	cellulose	1133:1141	arg1	films					1153:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	0	58	theme	carboxymethyl	59:71	arg1	cellulose					73:81	carboxymethyl cellulose	59:81	carboxymethyl cellulose	59:81	Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
31100395	1	59	theme	antioxidant	284:294	arg1	properties					336:345	antimicrobial, antioxidant, mechanical, physical and morphological properties	269:345	antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films	269:397	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	2	60	theme	Cross-linking	418:430	arg1	effect					432:437	Cross-linking effect	418:437	Cross-linking effect of GL (as determined by FTIR analysis)	418:476	Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP).
31100395	6	61	theme	chitosan-carboxymethyl	1110:1131	arg1	films					1153:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	chitosan-carboxymethyl cellulose composite films	1110:1157	We showed that the antimicrobial, antioxidant, mechanical and physical properties of chitosan-carboxymethyl cellulose composite films could be improved via incorporating CEO and GL to the film matrix.
31100395	1	62	theme	oleic	226:230	arg1	OA					238:239	OA	238:239	OA	238:239	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
31100395	1	62	theme	oleic	226:230	arg1	acid					232:235	oleic acid	226:235	oleic acid (OA)	226:240	In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated.
30242778	5	0	theme	mannitol	781:788	arg1	MHH					776:778	MHH	776:778	MHH (mannitol hemihydrate)	776:801	RESULT When colyophilized with sucrose, trehalose or lysozyme as a second solute, mannitol crystallized partially as MHH (mannitol hemihydrate).
30242778	5	0	theme	mannitol	781:788	arg1	hemihydrate					790:800	mannitol hemihydrate	781:800	mannitol hemihydrate	781:800	RESULT When colyophilized with sucrose, trehalose or lysozyme as a second solute, mannitol crystallized partially as MHH (mannitol hemihydrate).
30242778	8	1	dep	Annealing	1042:1050	arg1	eliminated					1101:1110	eliminated	1101:1110	eliminated	1101:1110	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	10	2	theme	composition	1459:1469	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	2	theme	composition	1459:1469	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	2	theme	composition	1459:1469	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	2	3	theme	formation	367:375	arg1	extent					330:335	the extent	326:335	the extent of mannitol hemihydrate (MHH) formation in the final lyophile	326:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	7	4	theme	cosolute	1024:1031	arg1	content					1033:1039	cosolute content	1024:1039	cosolute content	1024:1039	This heterogeneity, studied in detail in presence of sucrose, occurred irrespective of cosolute content.
30242778	0	5	from	Heterogeneity	11:23	arg1	Systems					78:84	Colyophilized Binary Systems	57:84	Colyophilized Binary Systems	57:84	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	0	5	from	Heterogeneity	11:23	arg1	Form					37:40	Physical Form	28:40	Physical Form of Mannitol	28:52	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	10	6	theme	formulation	1447:1457	arg1	composition					1459:1469	formulation composition	1447:1469	formulation composition	1447:1469	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	7	theme	final	1406:1410	arg1	lyophile					1412:1419	the final lyophile	1402:1419	the final lyophile	1402:1419	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	9	8	theme	differential	1187:1198	arg1	calorimetry					1209:1219	differential scanning calorimetry	1187:1219	differential scanning calorimetry	1187:1219	From differential scanning calorimetry it was evident that annealing caused mannitol crystallization while XRD revealed the crystallizing phase to be MHH.
30242778	11	9	theme	spatial	1534:1540	arg1	heterogeneity					1542:1554	spatial heterogeneity	1534:1554	spatial heterogeneity	1534:1554	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	8	10	theme	overall	1149:1155	arg1	increase					1157:1164	an overall increase	1146:1164	an overall increase in MHH content	1146:1179	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	9	11	theme	crystallizing	1306:1318	arg1	phase					1320:1324	the crystallizing phase	1302:1324	the crystallizing phase to be MHH	1302:1334	From differential scanning calorimetry it was evident that annealing caused mannitol crystallization while XRD revealed the crystallizing phase to be MHH.
30242778	3	12	from	composition	528:538	arg1	lyophiles					555:563	unperturbed lyophiles	543:563	unperturbed lyophiles	543:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	2	13	theme	conditions	312:321	arg1	influence					260:268	the influence	256:268	the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile	256:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	2	14	from	extent	330:335	arg1	lyophile					390:397	the final lyophile	380:397	the final lyophile	380:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	3	15	dep	METHODS	400:406	arg1	used					411:414	used	411:414	used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles	411:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	11	16	theme	hemihydrate	1568:1578	arg1	content					1580:1586	mannitol hemihydrate content	1559:1586	mannitol hemihydrate content	1559:1586	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	2	17	theme	processing	301:310	arg1	conditions					312:321	processing conditions	301:321	processing conditions	301:321	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	10	18	dep	CONCLUSION	1337:1346	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	18	dep	CONCLUSION	1337:1346	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	18	dep	CONCLUSION	1337:1346	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	9	19	from	calorimetry	1209:1219	arg1	evident					1228:1234	evident	1228:1234	evident	1228:1234	From differential scanning calorimetry it was evident that annealing caused mannitol crystallization while XRD revealed the crystallizing phase to be MHH.
30242778	11	20	from	heterogeneity	1542:1554	arg1	content					1580:1586	mannitol hemihydrate content	1559:1586	mannitol hemihydrate content	1559:1586	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	10	21	from	heterogeneity	1363:1375	arg1	lyophile					1412:1419	the final lyophile	1402:1419	the final lyophile	1402:1419	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	22	dep	heterogeneity	1363:1375	arg1	The					1348:1350	The	1348:1350	The	1348:1350	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	23	theme	processing	1475:1484	arg1	conditions					1486:1495	processing conditions	1475:1495	processing conditions	1475:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	3	24	theme	synchrotron	416:426	arg1	XRD					450:452	XRD	450:452	XRD	450:452	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	3	24	theme	synchrotron	416:426	arg1	diffractometry					434:447	synchrotron X-ray diffractometry	416:447	synchrotron X-ray diffractometry (XRD)	416:453	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	2	25	from	formation	367:375	arg1	lyophile					390:397	the final lyophile	380:397	the final lyophile	380:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	2	26	theme	composition	285:295	arg1	influence					260:268	the influence	256:268	the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile	256:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	1	27	theme	non-crystallizing	184:200	arg1	lyoprotectant					202:214	non-crystallizing lyoprotectant	184:214	non-crystallizing lyoprotectant	184:214	PURPOSE To study spatial heterogeneity in phase composition when mannitol is co-lyophilized with non-crystallizing lyoprotectant, such as sucrose or trehalose.
30242778	10	28	theme	complex	1426:1432	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	28	theme	complex	1426:1432	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	28	theme	complex	1426:1432	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	8	29	theme	frozen	1056:1061	arg1	solution					1063:1070	the frozen solution	1052:1070	the frozen solution at -30°C for 2 h	1052:1087	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	9	30	theme	scanning	1200:1207	arg1	calorimetry					1209:1219	differential scanning calorimetry	1187:1219	differential scanning calorimetry	1187:1219	From differential scanning calorimetry it was evident that annealing caused mannitol crystallization while XRD revealed the crystallizing phase to be MHH.
30242778	11	31	dep	such	1635:1638	arg1	as					1640:1641	as	1640:1641	as	1640:1641	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	0	32	theme	Intra-Vial	0:9	arg1	Heterogeneity					11:23	Intra-Vial Heterogeneity	0:23	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.	0:85	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	1	33	from	heterogeneity	112:124	arg1	composition					135:145	phase composition	129:145	phase composition	129:145	PURPOSE To study spatial heterogeneity in phase composition when mannitol is co-lyophilized with non-crystallizing lyoprotectant, such as sucrose or trehalose.
30242778	8	34	from	-30°C	1075:1079	arg1	solution					1063:1070	the frozen solution	1052:1070	the frozen solution at -30°C for 2 h	1052:1087	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	2	35	from	influence	260:268	arg1	extent					330:335	the extent	326:335	the extent of mannitol hemihydrate (MHH) formation in the final lyophile	326:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	2	36	theme	final	384:388	arg1	lyophile					390:397	the final lyophile	380:397	the final lyophile	380:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	0	37	theme	Physical	28:35	arg1	Form					37:40	Physical Form	28:40	Physical Form of Mannitol	28:52	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	4	38	theme	thermal	582:588	arg1	analysis					590:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	11	39	theme	mannitol	1559:1566	arg1	content					1580:1586	mannitol hemihydrate content	1559:1586	mannitol hemihydrate content	1559:1586	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	5	40	theme	second	726:731	arg1	mannitol					741:748	mannitol	741:748	mannitol	741:748	RESULT When colyophilized with sucrose, trehalose or lysozyme as a second solute, mannitol crystallized partially as MHH (mannitol hemihydrate).
30242778	5	40	theme	second	726:731	arg1	solute					733:738	a second solute	724:738	a second solute	724:738	RESULT When colyophilized with sucrose, trehalose or lysozyme as a second solute, mannitol crystallized partially as MHH (mannitol hemihydrate).
30242778	6	41	theme	d-spacing	872:880	arg1	peak					866:869	characteristic MHH peak	847:869	characteristic MHH peak (d-spacing 4.92 Å)	847:888	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	6	41	theme	d-spacing	872:880	arg1	4.92 Å					882:887	d-spacing 4.92 Å	872:887	d-spacing 4.92 Å	872:887	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	1	42	theme	spatial	104:110	arg1	heterogeneity					112:124	spatial heterogeneity	104:124	spatial heterogeneity in phase composition	104:145	PURPOSE To study spatial heterogeneity in phase composition when mannitol is co-lyophilized with non-crystallizing lyoprotectant, such as sucrose or trehalose.
30242778	4	43	theme	systems	650:656	arg1	behavior					631:638	phase behavior	625:638	phase behavior of frozen systems	625:656	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	3	44	theme	phase	522:526	arg1	composition					528:538	mannitol phase composition	513:538	mannitol phase composition in unperturbed lyophiles	513:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	10	45	from	content	1391:1397	arg1	lyophile					1412:1419	the final lyophile	1402:1419	the final lyophile	1402:1419	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	11	46	theme	Abstract	1508:1515	arg1	Figure					1517:1522	Graphical Abstract Figure	1498:1522	Graphical Abstract Figure	1498:1522	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	3	47	theme	X-ray	428:432	arg1	XRD					450:452	XRD	450:452	XRD	450:452	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	3	47	theme	X-ray	428:432	arg1	diffractometry					434:447	synchrotron X-ray diffractometry	416:447	synchrotron X-ray diffractometry (XRD)	416:453	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	6	48	theme	peak	866:869	arg1	intensity					834:842	the intensity	830:842	the intensity of characteristic MHH peak (d-spacing 4.92 Å)	830:888	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	2	49	from	lyophile	390:397	arg1	extent					330:335	the extent	326:335	the extent of mannitol hemihydrate (MHH) formation in the final lyophile	326:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	10	50	theme	conditions	1486:1495	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	50	theme	conditions	1486:1495	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	50	theme	conditions	1486:1495	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	6	51	theme	lyophile	927:934	arg1	region					917:922	the middle region	906:922	the middle region of lyophile	906:934	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	3	52	used	used	411:414	arg2	We					408:409	We	408:409	We	408:409	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	6	53	theme	MHH	862:864	arg1	peak					866:869	characteristic MHH peak	847:869	characteristic MHH peak (d-spacing 4.92 Å)	847:888	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	6	53	theme	MHH	862:864	arg1	4.92 Å					882:887	d-spacing 4.92 Å	872:887	d-spacing 4.92 Å	872:887	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	2	54	theme	MHH	362:364	arg1	formation					367:375	mannitol hemihydrate (MHH) formation	340:375	mannitol hemihydrate (MHH) formation in the final lyophile	340:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	0	55	theme	Mannitol	45:52	arg1	Form					37:40	Physical Form	28:40	Physical Form of Mannitol	28:52	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	4	56	theme	temperature	570:580	arg1	analysis					590:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	11	57	theme	Graphical	1498:1506	arg1	Figure					1517:1522	Graphical Abstract Figure	1498:1522	Graphical Abstract Figure	1498:1522	Graphical Abstract Figure depicting spatial heterogeneity in mannitol hemihydrate content, when mannitol is lyophilized with a cosolute, such as sucrose, trealose or lysozyme.
30242778	6	58	theme	characteristic	847:860	arg1	peak					866:869	characteristic MHH peak	847:869	characteristic MHH peak (d-spacing 4.92 Å)	847:888	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	6	58	theme	characteristic	847:860	arg1	4.92 Å					882:887	d-spacing 4.92 Å	872:887	d-spacing 4.92 Å	872:887	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	8	59	from	increase	1157:1164	arg1	content					1173:1179	MHH content	1169:1179	MHH content	1169:1179	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	3	60	theme	mannitol	513:520	arg1	composition					528:538	mannitol phase composition	513:538	mannitol phase composition in unperturbed lyophiles	513:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	4	61	theme	Low	566:568	arg1	analysis					590:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	6	62	theme	MHH	808:810	arg1	highest					895:901	highest	895:901	highest	895:901	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	6	62	theme	MHH	808:810	arg1	content					812:818	The MHH content	804:818	The MHH content	804:818	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	2	63	theme	formulation	273:283	arg1	composition					285:295	formulation composition	273:295	formulation composition	273:295	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	7	64	theme	sucrose	990:996	arg1	presence					978:985	presence	978:985	presence of sucrose	978:996	This heterogeneity, studied in detail in presence of sucrose, occurred irrespective of cosolute content.
30242778	1	65	theme	phase	129:133	arg1	composition					135:145	phase composition	129:145	phase composition	129:145	PURPOSE To study spatial heterogeneity in phase composition when mannitol is co-lyophilized with non-crystallizing lyoprotectant, such as sucrose or trehalose.
30242778	4	66	used	used	611:614	arg2	XRD					603:605	XRD	603:605	XRD	603:605	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	4	66	used	used	611:614	arg2	analysis					590:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis	566:597	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	8	67	theme	MHH	1169:1171	arg1	content					1173:1179	MHH content	1169:1179	MHH content	1169:1179	Annealing the frozen solution at -30°C for 2 h essentially eliminated the heterogeneity, accompanied by an overall increase in MHH content.
30242778	10	68	theme	MHH	1387:1389	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	68	theme	MHH	1387:1389	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	68	theme	MHH	1387:1389	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	2	69	theme	mannitol	340:347	arg1	formation					367:375	mannitol hemihydrate (MHH) formation	340:375	mannitol hemihydrate (MHH) formation in the final lyophile	340:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	10	70	theme	intra-vial	1352:1361	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	70	theme	intra-vial	1352:1361	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	70	theme	intra-vial	1352:1361	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	2	71	theme	hemihydrate	349:359	arg1	formation					367:375	mannitol hemihydrate (MHH) formation	340:375	mannitol hemihydrate (MHH) formation in the final lyophile	340:397	To study the influence of formulation composition and processing conditions on the extent of mannitol hemihydrate (MHH) formation in the final lyophile.
30242778	0	72	theme	Binary	71:76	arg1	Systems					78:84	Colyophilized Binary Systems	57:84	Colyophilized Binary Systems	57:84	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	3	73	dep	map	468:470	arg1	characterize					500:511	characterize	500:511	characterize mannitol phase composition in unperturbed lyophiles	500:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30242778	10	74	theme	total	1381:1385	arg1	content					1391:1397	total MHH content	1381:1397	total MHH content	1381:1397	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	74	theme	total	1381:1385	arg1	interplay					1434:1442	a complex interplay	1424:1442	a complex interplay of formulation composition and processing conditions	1424:1495	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	10	74	theme	total	1381:1385	arg1	heterogeneity					1363:1375	intra-vial heterogeneity	1352:1375	intra-vial heterogeneity	1352:1375	CONCLUSION The intra-vial heterogeneity and total MHH content in the final lyophile is a complex interplay of formulation composition and processing conditions.
30242778	9	75	theme	mannitol	1258:1265	arg1	crystallization					1267:1281	mannitol crystallization	1258:1281	mannitol crystallization	1258:1281	From differential scanning calorimetry it was evident that annealing caused mannitol crystallization while XRD revealed the crystallizing phase to be MHH.
30242778	4	76	theme	frozen	643:648	arg1	systems					650:656	frozen systems	643:656	frozen systems	643:656	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	0	77	theme	Colyophilized	57:69	arg1	Systems					78:84	Colyophilized Binary Systems	57:84	Colyophilized Binary Systems	57:84	Intra-Vial Heterogeneity in Physical Form of Mannitol in Colyophilized Binary Systems.
30242778	6	78	theme	middle	910:915	arg1	region					917:922	the middle region	906:922	the middle region of lyophile	906:934	The MHH content, based on the intensity of characteristic MHH peak (d-spacing 4.92 Å), was highest in the middle region of lyophile.
30242778	4	79	theme	phase	625:629	arg1	behavior					631:638	phase behavior	625:638	phase behavior of frozen systems	625:656	Low temperature thermal analysis and XRD was used to study phase behavior of frozen systems.
30242778	3	80	theme	unperturbed	543:553	arg1	lyophiles					555:563	unperturbed lyophiles	543:563	unperturbed lyophiles	543:563	METHODS We used synchrotron X-ray diffractometry (XRD) to spatially map and thereby comprehensively characterize mannitol phase composition in unperturbed lyophiles.
30862514	11	0	theme	high	1943:1946	arg1	compositions					1958:1969	high drug load compositions	1943:1969	high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing	1943:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	10	1	from	funnel	1743:1748	arg1	inlet					1763:1767	the mixer inlet	1753:1767	the mixer inlet	1753:1767	In addition, some of the API became stuck in the CDC line between feeding and tableting, most likely at the funnel in the mixer inlet, highlighting the need for properly designed interfaces between units.
30862514	9	2	theme	low	1573:1575	arg1	rate					1582:1585	a low feed rate	1571:1585	a low feed rate for the API proved challenging in the CDC line	1571:1632	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	6	3	with	formulations	1051:1062	arg1	content					1080:1086	a high API content	1069:1086	a high API content	1069:1086	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	11	4	theme	blends	1996:2001	arg1	compositions					1958:1969	high drug load compositions	1943:1969	high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing	1943:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	6	5	theme	equal	1121:1125	arg1	uniformity					1127:1136	better or equal uniformity	1111:1136	better or equal uniformity of content and tablet weight	1111:1165	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	3	6	theme	Ingredient	724:733	arg1	grades					688:693	different grades	678:693	different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler	678:770	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	7	theme	study	277:281	arg1	purpose					261:267	The purpose	257:267	The purpose of this study	257:281	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	7	8	dep	standard	1322:1329	arg1	cohesive					1332:1339	cohesive	1332:1339	cohesive	1332:1339	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	11	9	theme	flowing	1981:1987	arg1	blends					1996:2001	poorly flowing powder blends	1974:2001	poorly flowing powder blends that could not be processed via batch manufacturing	1974:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	6	10	theme	better	1111:1116	arg1	uniformity					1127:1136	better or equal uniformity	1111:1136	better or equal uniformity of content and tablet weight	1111:1165	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	11	11	theme	drug	1948:1951	arg1	compositions					1958:1969	high drug load compositions	1943:1969	high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing	1943:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	0	12	theme	properties	115:124	arg1	effect					92:97	The effect	88:97	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.	0:125	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	1	13	theme	traditional	198:208	arg1	manufacture					216:226	traditional batch manufacture	198:226	traditional batch manufacture	198:226	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	10	14	theme	CDC	1684:1686	arg1	line					1688:1691	the CDC line	1680:1691	the CDC line between feeding and tableting	1680:1721	In addition, some of the API became stuck in the CDC line between feeding and tableting, most likely at the funnel in the mixer inlet, highlighting the need for properly designed interfaces between units.
30862514	9	15	theme	CDC	1625:1627	arg1	line					1629:1632	the CDC line	1621:1632	the CDC line	1621:1632	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	3	16	theme	major	759:763	arg1	filler					765:770	major filler	759:770	major filler	759:770	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	11	17	theme	load	1953:1956	arg1	compositions					1958:1969	high drug load compositions	1943:1969	high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing	1943:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	6	18	theme	weight	1160:1165	arg1	uniformity					1127:1136	better or equal uniformity	1111:1136	better or equal uniformity of content and tablet weight	1111:1165	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	5	19	theme	batch	1024:1028	arg1	process					1030:1036	the batch process	1020:1036	the batch process	1020:1036	The overall process stability over time was significantly improved with the CDC line as compared to the batch process.
30862514	3	20	theme	segregation	622:632	arg1	indices					634:640	different segregation indices	612:640	different segregation indices	612:640	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	21	theme	poor	487:490	arg1	properties					497:506	poor flow properties	487:506	poor flow properties	487:506	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	0	22	theme	batch	69:73	arg1	processing					75:84	batch processing	69:84	batch processing	69:84	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	1	23	theme	current	138:144	arg1	trend					146:150	a current trend	136:150	a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing	136:254	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	2	24	from	ability	299:305	arg1	relation					369:376	relation	369:376	relation to batch processing	369:396	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	4	25	theme	tablet	884:889	arg1	weight					891:896	tablet weight	884:896	tablet weight	884:896	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	1	26	from	trend	146:150	arg1	manufacturing					170:182	pharmaceutical manufacturing	155:182	pharmaceutical manufacturing	155:182	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	5	27	theme	process	932:938	arg1	stability					940:948	The overall process stability	920:948	The overall process stability over time	920:958	The overall process stability over time was significantly improved with the CDC line as compared to the batch process.
30862514	8	28	theme	low	1447:1449	arg1	content					1455:1461	a low API content	1445:1461	a low API content	1445:1461	The only formulation that performed better in the batch process was the formulation with a low API content.
30862514	0	29	theme	raw	102:104	arg1	properties					115:124	raw material properties	102:124	raw material properties	102:124	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	8	30	with	formulation	1428:1438	arg1	content					1455:1461	a low API content	1445:1461	a low API content	1445:1461	The only formulation that performed better in the batch process was the formulation with a low API content.
30862514	7	31	theme	tablet	1261:1266	arg1	weight					1268:1273	tablet weight	1261:1273	tablet weight	1261:1273	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	2	32	theme	consistent	410:419	arg1	quality					428:434	consistent tablet quality	410:434	consistent tablet quality	410:434	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	0	33	theme	integrated	19:28	arg1	line					60:63	integrated continuous direct compression line	19:63	integrated continuous direct compression line	19:63	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	9	34	theme	batch	1496:1500	arg1	process					1502:1508	the batch process	1492:1508	the batch process	1492:1508	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	0	35	theme	direct	41:46	arg1	line					60:63	integrated continuous direct compression line	19:63	integrated continuous direct compression line	19:63	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	2	36	theme	long	441:444	arg1	periods					457:463	long processing periods	441:463	long processing periods for formulations with poor flow properties or with a tendency to segregate	441:538	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	2	37	theme	compression	342:352	arg1	line					360:363	an integrated continuous direct compression (CDC) line	310:363	an integrated continuous direct compression (CDC) line	310:363	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	6	38	theme	content	1141:1147	arg1	uniformity					1127:1136	better or equal uniformity	1111:1136	better or equal uniformity of content and tablet weight	1111:1165	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	2	39	theme	continuous	324:333	arg1	CDC					355:357	CDC	355:357	CDC	355:357	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	2	39	theme	continuous	324:333	arg1	compression					342:352	continuous direct compression	324:352	an integrated continuous direct compression (CDC) line	310:363	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	11	40	theme	CDC	1865:1867	arg1	line					1869:1872	the CDC line	1861:1872	the CDC line	1861:1872	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	9	41	theme	tablet	1538:1543	arg1	content					1545:1551	tablet content	1538:1551	tablet content	1538:1551	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	6	42	theme	API	1076:1078	arg1	content					1080:1086	a high API content	1069:1086	a high API content	1069:1086	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	2	43	theme	line	360:363	arg1	ability					299:305	the ability	295:305	the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate	295:538	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	7	44	contain	containing	1307:1316	arg1	formulations					1294:1305	poorly flowing formulations	1279:1305	poorly flowing formulations containing the standard, cohesive, grade of API	1279:1353	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	7	44	contain	containing	1307:1316	arg2	grade					1342:1346	the standard, cohesive, grade	1318:1346	grade	1342:1346	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	0	45	dep	Comparison	0:9	arg1	effect					92:97	The effect	88:97	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.	0:125	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	3	46	theme	Pharmaceutical	709:722	arg1	API					736:738	API	736:738	API	736:738	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	3	46	theme	Pharmaceutical	709:722	arg1	Ingredient					724:733	the Active Pharmaceutical Ingredient	698:733	the Active Pharmaceutical Ingredient (API)	698:739	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	10	47	theme	mixer	1757:1761	arg1	inlet					1763:1767	the mixer inlet	1753:1767	the mixer inlet	1753:1767	In addition, some of the API became stuck in the CDC line between feeding and tableting, most likely at the funnel in the mixer inlet, highlighting the need for properly designed interfaces between units.
30862514	11	48	theme	line	1869:1872	arg1	insensitivity					1844:1856	The insensitivity	1840:1856	The insensitivity of the CDC line towards poor flow	1840:1890	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	9	49	theme	feed	1577:1580	arg1	rate					1582:1585	a low feed rate	1571:1585	a low feed rate for the API proved challenging in the CDC line	1571:1632	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	1	50	theme	pharmaceutical	155:168	arg1	manufacturing					170:182	pharmaceutical manufacturing	155:182	pharmaceutical manufacturing	155:182	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	4	51	theme	performance	814:824	arg1	metrics					826:832	The performance metrics	810:832	The performance metrics investigated	810:845	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	4	51	theme	performance	814:824	arg1	content					852:858	content	852:858	content	852:858	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	3	52	theme	study	545:549	arg1	design					551:556	The study design	541:556	The study design	541:556	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	11	53	theme	powder	1989:1994	arg1	blends					1996:2001	poorly flowing powder blends	1974:2001	poorly flowing powder blends that could not be processed via batch manufacturing	1974:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	2	54	from	line	360:363	arg1	relation					369:376	relation	369:376	relation to batch processing	369:396	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	7	55	theme	API	1351:1353	arg1	grade					1342:1346	the standard, cohesive, grade	1318:1346	grade	1342:1346	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	11	56	theme	poor	1882:1885	arg1	flow					1887:1890	poor flow	1882:1890	poor flow	1882:1890	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	2	57	with	formulations	469:480	arg1	properties					497:506	poor flow properties	487:506	poor flow properties	487:506	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	2	57	with	formulations	469:480	arg1	tendency					518:525	a tendency	516:525	a tendency to segregate	516:538	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	11	58	theme	direct	1921:1926	arg1	compression					1928:1938	direct compression	1921:1938	direct compression	1921:1938	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	6	59	theme	CDC	1093:1095	arg1	line					1097:1100	the CDC line	1089:1100	the CDC line	1089:1100	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	10	60	theme	designed	1805:1812	arg1	interfaces					1814:1823	properly designed interfaces	1796:1823	properly designed interfaces between units	1796:1837	In addition, some of the API became stuck in the CDC line between feeding and tableting, most likely at the funnel in the mixer inlet, highlighting the need for properly designed interfaces between units.
30862514	1	61	theme	batch	210:214	arg1	manufacture					216:226	traditional batch manufacture	198:226	traditional batch manufacture	198:226	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	4	62	theme	tablet	903:908	arg1	strength					910:917	tablet strength	903:917	tablet strength	903:917	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	1	63	from	manufacture	216:226	arg1	shift					187:191	shift	187:191	shift from traditional batch manufacture to continuous manufacturing	187:254	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	4	64	theme	content	875:881	arg1	weight					891:896	tablet weight	884:896	tablet weight	884:896	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	4	64	theme	content	875:881	arg1	uniformity					861:870	uniformity	861:870	uniformity of content	861:881	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	4	64	theme	content	875:881	arg1	metrics					826:832	The performance metrics	810:832	The performance metrics investigated	810:845	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	4	64	theme	content	875:881	arg1	content					852:858	content	852:858	content	852:858	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	4	64	theme	content	875:881	arg1	strength					910:917	tablet strength	903:917	tablet strength	903:917	The performance metrics investigated were content, uniformity of content, tablet weight, and tablet strength.
30862514	8	65	theme	batch	1406:1410	arg1	process					1412:1418	the batch process	1402:1418	the batch process	1402:1418	The only formulation that performed better in the batch process was the formulation with a low API content.
30862514	7	66	theme	standard	1322:1329	arg1	grade					1342:1346	the standard, cohesive, grade	1318:1346	grade	1342:1346	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	3	67	theme	filler	765:770	arg1	grades					688:693	different grades	678:693	different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler	678:770	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	3	68	theme	different	612:620	arg1	indices					634:640	different segregation indices	612:640	different segregation indices	612:640	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	69	from	relation	369:376	arg1	ability					299:305	the ability	295:305	the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate	295:538	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	6	70	theme	tablet	1153:1158	arg1	weight					1160:1165	tablet weight	1153:1165	tablet weight	1153:1165	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	7	71	theme	flowing	1286:1292	arg1	formulations					1294:1305	poorly flowing formulations	1279:1305	poorly flowing formulations containing the standard, cohesive, grade of API	1279:1353	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	8	72	theme	API	1451:1453	arg1	content					1455:1461	a low API content	1445:1461	a low API content	1445:1461	The only formulation that performed better in the batch process was the formulation with a low API content.
30862514	3	73	theme	API	805:807	arg1	API					805:807	API	805:807	API	805:807	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	3	73	theme	API	805:807	arg1	amount					795:800	the amount	791:800	the amount of API	791:807	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	0	74	theme	material	106:113	arg1	properties					115:124	raw material properties	102:124	raw material properties	102:124	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	2	75	theme	flow	492:495	arg1	properties					497:506	poor flow properties	487:506	poor flow properties	487:506	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	3	76	theme	paracetamol	742:752	arg1	grades					688:693	different grades	678:693	different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler	678:770	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	77	theme	tablet	421:426	arg1	quality					428:434	consistent tablet quality	410:434	consistent tablet quality	410:434	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	0	78	theme	continuous	30:39	arg1	line					60:63	integrated continuous direct compression line	19:63	integrated continuous direct compression line	19:63	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	5	79	theme	overall	924:930	arg1	stability					940:948	The overall process stability	920:948	The overall process stability over time	920:958	The overall process stability over time was significantly improved with the CDC line as compared to the batch process.
30862514	9	80	from	variation	1525:1533	arg1	content					1545:1551	tablet content	1538:1551	tablet content	1538:1551	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	7	81	theme	stable	1242:1247	arg1	content					1249:1255	a stable content	1240:1255	a stable content	1240:1255	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	3	82	theme	flow	646:649	arg1	properties					651:660	flow properties	646:660	flow properties	646:660	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	6	83	theme	high	1071:1074	arg1	content					1080:1086	a high API content	1069:1086	a high API content	1069:1086	For all the formulations with a high API content, the CDC line provided better or equal uniformity of content and tablet weight as compared to batch.
30862514	0	84	theme	compression	48:58	arg1	line					60:63	integrated continuous direct compression line	19:63	integrated continuous direct compression line	19:63	Comparison between integrated continuous direct compression line and batch processing - The effect of raw material properties.
30862514	5	85	theme	CDC	996:998	arg1	line					1000:1003	the CDC line	992:1003	the CDC line	992:1003	The overall process stability over time was significantly improved with the CDC line as compared to the batch process.
30862514	2	86	theme	processing	446:455	arg1	periods					457:463	long processing periods	441:463	long processing periods for formulations with poor flow properties or with a tendency to segregate	441:538	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	2	87	theme	batch	381:385	arg1	processing					387:396	batch processing	381:396	batch processing	381:396	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	9	88	theme	lower	1519:1523	arg1	variation					1525:1533	lower variation	1519:1533	lower variation in tablet content	1519:1551	Thus, for this formulation, the batch process achieved lower variation in tablet content since maintaining a low feed rate for the API proved challenging in the CDC line.
30862514	11	89	theme	batch	2035:2039	arg1	manufacturing					2041:2053	batch manufacturing	2035:2053	batch manufacturing	2035:2053	The insensitivity of the CDC line towards poor flow indicates that one could use direct compression at high drug load compositions of poorly flowing powder blends that could not be processed via batch manufacturing.
30862514	3	90	theme	different	678:686	arg1	grades					688:693	different grades	678:693	different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler	678:770	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	91	theme	direct	335:340	arg1	CDC					355:357	CDC	355:357	CDC	355:357	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	2	91	theme	direct	335:340	arg1	compression					342:352	continuous direct compression	324:352	an integrated continuous direct compression (CDC) line	310:363	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	3	92	theme	relevant	585:592	arg1	formulations					594:605	four industrially relevant formulations	567:605	four industrially relevant formulations	567:605	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	2	93	theme	integrated	313:322	arg1	line					360:363	an integrated continuous direct compression (CDC) line	310:363	an integrated continuous direct compression (CDC) line	310:363	The purpose of this study was to test the ability of an integrated continuous direct compression (CDC) line, in relation to batch processing, to achieve consistent tablet quality over long processing periods for formulations with poor flow properties or with a tendency to segregate.
30862514	1	94	theme	continuous	231:240	arg1	manufacturing					242:254	continuous manufacturing	231:254	continuous manufacturing	231:254	There is a current trend in pharmaceutical manufacturing to shift from traditional batch manufacture to continuous manufacturing.
30862514	7	95	theme	CDC	1193:1195	arg1	line					1197:1200	The CDC line	1189:1200	The CDC line	1189:1200	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	7	95	theme	CDC	1193:1195	arg1	efficient					1217:1225	efficient	1217:1225	efficient	1217:1225	The CDC line was especially efficient in providing a stable content and tablet weight for poorly flowing formulations containing the standard, cohesive, grade of API.
30862514	3	96	theme	Active	702:707	arg1	API					736:738	API	736:738	API	736:738	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	3	96	theme	Active	702:707	arg1	Ingredient					724:733	the Active Pharmaceutical Ingredient	698:733	the Active Pharmaceutical Ingredient (API)	698:739	The study design included four industrially relevant formulations with different segregation indices and flow properties induced through different grades of the Active Pharmaceutical Ingredient (API), paracetamol, and major filler as well as varying the amount of API.
30862514	8	97	theme	only	1360:1363	arg1	formulation					1428:1438	the formulation	1424:1438	the formulation	1424:1438	The only formulation that performed better in the batch process was the formulation with a low API content.
30862514	8	97	theme	only	1360:1363	arg1	formulation					1365:1375	The only formulation	1356:1375	The only formulation that performed better in the batch process	1356:1418	The only formulation that performed better in the batch process was the formulation with a low API content.
30208257	3	0	theme	nuclear	627:633	arg1	resonance					644:652	nuclear magnetic resonance	627:652	nuclear magnetic resonance	627:652	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	6	1	theme	advanced	1189:1196	arg1	material					1208:1215	an advanced composite material	1186:1215	an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation	1186:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	7	2	theme	modulus	1407:1413	arg1	expression					1476:1485	an early alkaline phosphatase expression	1446:1485	an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium	1446:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	7	2	theme	modulus	1407:1413	arg1	increase					1383:1390	a 10-time increase	1373:1390	a 10-time increase of the storage modulus, if compared to neat MeHA,	1373:1440	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	3	3	theme	molar	515:519	arg1	ratios					521:526	MA/HA molar ratios	509:526	MA/HA molar ratios	509:526	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	8	4	theme	photo-cross-linkable	1615:1634	arg1	hydrogels					1636:1644	photo-cross-linkable hydrogels	1615:1644	photo-cross-linkable hydrogels	1615:1644	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	4	5	theme	polymer	765:771	arg1	concentration					773:785	the polymer concentration	761:785	the polymer concentration	761:785	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	7	6	theme	composite-DN	1343:1354	arg1	hydrogels					1356:1364	composite-DN hydrogels	1343:1364	composite-DN hydrogels	1343:1364	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	3	7	theme	magnetic	635:642	arg1	resonance					644:652	nuclear magnetic resonance	627:652	nuclear magnetic resonance	627:652	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	6	8	theme	hydroxyapatite	1069:1082	arg1	HAp					1099:1101	HAp	1099:1101	HAp	1099:1101	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	8	theme	hydroxyapatite	1069:1082	arg1	nanoparticles					1084:1096	hydroxyapatite nanoparticles	1069:1096	hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1069:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	4	9	dep	DS	719:720	arg1	the					715:717	the	715:717	the	715:717	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	3	10	theme	total	669:673	arg1	spectroscopy					686:697	attenuated total reflection spectroscopy	658:697	attenuated total reflection spectroscopy	658:697	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	1	11	theme	promising	180:188	arg1	biomaterials					208:219	the most promising naturally derived biomaterials	171:219	the most promising naturally derived biomaterials for tissue engineering applications	171:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	8	12	theme	natural/synthetic	1673:1689	arg1	polymers					1691:1698	natural/synthetic polymers	1673:1698	natural/synthetic polymers	1673:1698	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	1	13	theme	derived	200:206	arg1	biomaterials					208:219	the most promising naturally derived biomaterials	171:219	the most promising naturally derived biomaterials for tissue engineering applications	171:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	6	14	theme	dual	1222:1225	arg1	function					1227:1234	dual function	1222:1234	dual function in terms of mechanical and biological support for soft/hard tissue formation	1222:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	5	15	theme	diacrylate	963:972	arg1	use					926:928	the synergic use	913:928	the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA)	913:980	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	3	16	theme	different	547:555	arg1	degrees					557:563	different degrees	547:563	different degrees of substitution (DS) ranging from 3.5% to 74.5%	547:611	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	1	17	theme	Hyaluronic	123:132	arg1	acid					134:137	Hyaluronic acid	123:137	Hyaluronic acid (HA)-based hydrogels	123:158	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	1	17	theme	Hyaluronic	123:132	arg1	HA					140:141	HA	140:141	HA	140:141	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	7	18	theme	basal	1524:1528	arg1	medium					1530:1535	basal medium	1524:1535	basal medium	1524:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	5	19	theme	Double	861:866	arg1	hydrogels					881:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	0	20	theme	tailored	59:66	arg1	features					113:120	tailored mechanical, physico-chemical, and biological features	59:120	tailored mechanical, physico-chemical, and biological features	59:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	1	21	theme	cellular	305:312	arg1	processes					314:322	many key cellular processes	296:322	many key cellular processes	296:322	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	4	22	dep	tuning	708:713	arg1	concentration					773:785	the polymer concentration	761:785	the polymer concentration	761:785	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	4	22	dep	tuning	708:713	arg1	parameters					745:754	the chemical reaction parameters	723:754	the chemical reaction parameters	723:754	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	4	22	dep	tuning	708:713	arg1	DS					719:720	DS	719:720	DS	719:720	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	7	23	theme	mesenchymal	1498:1508	arg1	cells					1515:1519	human mesenchymal stem cells	1492:1519	human mesenchymal stem cells	1492:1519	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	2	24	theme	hyaluronic	474:483	arg1	acid					485:488	methacrylated hyaluronic acid	460:488	methacrylated hyaluronic acid (MeHA)	460:495	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	2	24	theme	hyaluronic	474:483	arg1	MeHA					491:494	MeHA	491:494	MeHA	491:494	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	6	25	theme	soft/hard	1286:1294	arg1	formation					1303:1311	soft/hard tissue formation	1286:1311	soft/hard tissue formation	1286:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	4	26	theme	fine	703:706	arg1	tuning					708:713	fine tuning the DS, the chemical reaction parameters, and the polymer concentration	703:785	fine tuning the DS, the chemical reaction parameters, and the polymer concentration	703:785	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	6	27	theme	support	1274:1280	arg1	terms					1239:1243	terms	1239:1243	terms of mechanical and biological support for soft/hard tissue formation	1239:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	8	28	theme	three-dimensional	1734:1750	arg1	techniques					1775:1784	three-dimensional additive manufacturing techniques	1734:1784	three-dimensional additive manufacturing techniques such as stereolithography	1734:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	8	28	theme	three-dimensional	1734:1750	arg1	stereolithography					1794:1810	stereolithography	1794:1810	stereolithography	1794:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	7	29	theme	phosphatase	1464:1474	arg1	expression					1476:1485	an early alkaline phosphatase expression	1446:1485	an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium	1446:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	10	30	dep	106A	1871:1874	arg1	3079-3089					1877:1885	3079-3089	1877:1885	3079-3089	1877:1885	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.
30208257	0	31	theme	biological	102:111	arg1	features					113:120	tailored mechanical, physico-chemical, and biological features	59:120	tailored mechanical, physico-chemical, and biological features	59:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	8	32	theme	manufacturing	1761:1773	arg1	techniques					1775:1784	three-dimensional additive manufacturing techniques	1734:1784	three-dimensional additive manufacturing techniques such as stereolithography	1734:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	8	32	theme	manufacturing	1761:1773	arg1	stereolithography					1794:1810	stereolithography	1794:1810	stereolithography	1794:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	0	33	theme	Bioactive	0:8	arg1	composites					10:19	Bioactive composites	0:19	Bioactive composites	0:19	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	2	34	theme	methacrylic	423:433	arg1	anhydride					435:443	methacrylic anhydride	423:443	methacrylic anhydride (MA)	423:448	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	2	34	theme	methacrylic	423:433	arg1	MA					446:447	MA	446:447	MA	446:447	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	10	35	dep	Res	1859:1861	arg1	106A					1871:1874	106A	1871:1874	106A	1871:1874	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.
30208257	10	35	dep	Res	1859:1861	arg1	A					1868:1868	Part A	1863:1868	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.	1844:1892	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.
30208257	0	36	theme	double	30:35	arg1	approach					45:52	double network approach	30:52	double network approach with tailored mechanical, physico-chemical, and biological features	30:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	7	37	theme	human	1492:1496	arg1	cells					1515:1519	human mesenchymal stem cells	1492:1519	human mesenchymal stem cells	1492:1519	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	10	38	theme	Part	1863:1866	arg1	A					1868:1868	Part A	1863:1868	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.	1844:1892	J Biomed Mater Res Part A: 106A: 3079-3089, 2018.
30208257	6	39	from	function	1227:1234	arg1	terms					1239:1243	terms	1239:1243	terms of mechanical and biological support for soft/hard tissue formation	1239:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	7	40	theme	neat	1431:1434	arg1	MeHA					1436:1439	neat MeHA	1431:1439	neat MeHA	1431:1439	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	5	41	theme	glycole	955:961	arg1	PEGDA					975:979	PEGDA	975:979	PEGDA	975:979	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	5	41	theme	glycole	955:961	arg1	diacrylate					963:972	polyethylene glycole diacrylate	942:972	polyethylene glycole diacrylate (PEGDA)	942:980	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	6	42	theme	bioactive	1037:1045	arg1	signals					1053:1059	bioactive solid signals	1037:1059	bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1037:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	42	theme	bioactive	1037:1045	arg1	nanoparticles					1084:1096	hydroxyapatite nanoparticles	1069:1096	hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1069:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	1	43	theme	biomaterials	208:219	arg1	biomaterials					208:219	the most promising naturally derived biomaterials	171:219	the most promising naturally derived biomaterials for tissue engineering applications	171:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	1	43	theme	biomaterials	208:219	arg1	one					164:166	one	164:166	one	164:166	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	7	44	from	cells	1515:1519	arg1	expression					1476:1485	an early alkaline phosphatase expression	1446:1485	an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium	1446:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	7	44	from	cells	1515:1519	arg1	increase					1383:1390	a 10-time increase	1373:1390	a 10-time increase of the storage modulus, if compared to neat MeHA,	1373:1440	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	5	45	theme	synergic	917:924	arg1	use					926:928	the synergic use	913:928	the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA)	913:980	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	6	46	theme	hydrogels	1026:1034	arg1	properties					1009:1018	the biological properties	994:1018	the biological properties of DN hydrogels	994:1034	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	4	47	theme	chemical	727:734	arg1	parameters					745:754	the chemical reaction parameters	723:754	the chemical reaction parameters	723:754	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	0	48	with	approach	45:52	arg1	features					113:120	tailored mechanical, physico-chemical, and biological features	59:120	tailored mechanical, physico-chemical, and biological features	59:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	8	49	theme	first	1568:1572	arg1	approach					1585:1592	a first systematic approach	1566:1592	a first systematic approach	1566:1592	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	1	50	theme	tissue	225:230	arg1	applications					244:255	tissue engineering applications	225:255	tissue engineering applications	225:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	6	51	theme	tissue	1296:1301	arg1	formation					1303:1311	soft/hard tissue formation	1286:1311	soft/hard tissue formation	1286:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	52	theme	composite	1198:1206	arg1	material					1208:1215	an advanced composite material	1186:1215	an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation	1186:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	53	used	used	1138:1141	arg2	signals					1053:1059	bioactive solid signals	1037:1059	bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1037:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	53	used	used	1138:1141	arg2	nanoparticles					1084:1096	hydroxyapatite nanoparticles	1069:1096	hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1069:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	7	54	theme	storage	1399:1405	arg1	modulus					1407:1413	the storage modulus	1395:1413	the storage modulus	1395:1413	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	6	55	theme	biological	998:1007	arg1	properties					1009:1018	the biological properties	994:1018	the biological properties of DN hydrogels	994:1034	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	3	56	theme	MA/HA	509:513	arg1	ratios					521:526	MA/HA molar ratios	509:526	MA/HA molar ratios	509:526	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	3	57	theme	attenuated	658:667	arg1	spectroscopy					686:697	attenuated total reflection spectroscopy	658:697	attenuated total reflection spectroscopy	658:697	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	7	58	theme	10-time	1375:1381	arg1	increase					1383:1390	a 10-time increase	1373:1390	a 10-time increase of the storage modulus, if compared to neat MeHA,	1373:1440	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	1	59	theme	many	296:299	arg1	processes					314:322	many key cellular processes	296:322	many key cellular processes	296:322	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	6	60	theme	DN	1163:1164	arg1	hydrogels					1166:1174	DN hydrogels	1163:1174	DN hydrogels	1163:1174	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	7	61	from	increase	1383:1390	arg1	medium					1530:1535	basal medium	1524:1535	basal medium	1524:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	6	62	theme	mechanical	1248:1257	arg1	support					1274:1280	mechanical and biological support	1248:1280	mechanical and biological support for soft/hard tissue formation	1248:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	3	63	theme	ratios	521:526	arg1	range					500:504	A range	498:504	A range of MA/HA molar ratios	498:526	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	6	64	with	material	1208:1215	arg1	function					1227:1234	dual function	1222:1234	dual function in terms of mechanical and biological support for soft/hard tissue formation	1222:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	4	65	theme	mechanical	840:849	arg1	features					851:858	their mechanical features	834:858	their mechanical features	834:858	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	3	66	theme	reflection	675:684	arg1	spectroscopy					686:697	attenuated total reflection spectroscopy	658:697	attenuated total reflection spectroscopy	658:697	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	3	67	used	used	532:535	arg2	range					500:504	A range	498:504	A range of MA/HA molar ratios	498:526	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	8	68	theme	polymers	1691:1698	arg1	combination					1658:1668	a combination	1656:1668	a combination of natural/synthetic polymers and HAp	1656:1706	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	1	69	theme	acid	134:137	arg1	hydrogels					150:158	Hyaluronic acid (HA)-based hydrogels	123:158	Hyaluronic acid (HA)-based hydrogels	123:158	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	7	70	theme	stem	1510:1513	arg1	cells					1515:1519	human mesenchymal stem cells	1492:1519	human mesenchymal stem cells	1492:1519	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	6	71	theme	sol-gel	1116:1122	arg1	approach					1124:1131	sol-gel approach	1116:1131	sol-gel approach	1116:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	0	72	theme	mechanical	68:77	arg1	features					113:120	tailored mechanical, physico-chemical, and biological features	59:120	tailored mechanical, physico-chemical, and biological features	59:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	3	73	theme	substitution	568:579	arg1	degrees					557:563	different degrees	547:563	different degrees of substitution (DS) ranging from 3.5% to 74.5%	547:611	A range of MA/HA molar ratios was used to obtain different degrees of substitution (DS) ranging from 3.5% to 74.5%, as showed by nuclear magnetic resonance and attenuated total reflection spectroscopy.
30208257	8	74	theme	HAp	1704:1706	arg1	combination					1658:1668	a combination	1656:1668	a combination of natural/synthetic polymers and HAp	1656:1706	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	6	75	with	combination	1146:1156	arg1	hydrogels					1166:1174	DN hydrogels	1163:1174	DN hydrogels	1163:1174	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	0	76	theme	physico-chemical	80:95	arg1	features					113:120	tailored mechanical, physico-chemical, and biological features	59:120	tailored mechanical, physico-chemical, and biological features	59:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	1	77	theme	-based	143:148	arg1	hydrogels					150:158	Hyaluronic acid (HA)-based hydrogels	123:158	Hyaluronic acid (HA)-based hydrogels	123:158	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	2	78	theme	methacrylated	460:472	arg1	acid					485:488	methacrylated hyaluronic acid	460:488	methacrylated hyaluronic acid (MeHA)	460:495	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	2	78	theme	methacrylated	460:472	arg1	MeHA					491:494	MeHA	491:494	MeHA	491:494	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	1	79	link	derived	200:206	arg1	biomaterials					208:219	the most promising naturally derived biomaterials	171:219	the most promising naturally derived biomaterials for tissue engineering applications	171:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	9	80	dep	©	1813:1813	arg1	Inc.					1839:1842	Inc.	1839:1842	Inc.	1839:1842	© 2018 Wiley Periodicals, Inc.
30208257	7	81	theme	alkaline	1455:1462	arg1	phosphatase					1464:1474	an early alkaline phosphatase	1446:1474	an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium	1446:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	6	82	theme	biological	1263:1272	arg1	support					1274:1280	mechanical and biological support	1248:1280	mechanical and biological support for soft/hard tissue formation	1248:1311	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	8	83	theme	additive	1752:1759	arg1	techniques					1775:1784	three-dimensional additive manufacturing techniques	1734:1784	three-dimensional additive manufacturing techniques such as stereolithography	1734:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	8	83	theme	additive	1752:1759	arg1	stereolithography					1794:1810	stereolithography	1794:1810	stereolithography	1794:1810	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	5	84	theme	network	868:874	arg1	hydrogels					881:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	7	85	dep	modulus	1407:1413	arg1	compared					1419:1426	compared	1419:1426	compared to neat MeHA	1419:1439	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	5	86	theme	DN	877:878	arg1	hydrogels					881:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels	861:889	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	0	87	theme	network	37:43	arg1	approach					45:52	double network approach	30:52	double network approach with tailored mechanical, physico-chemical, and biological features	30:120	Bioactive composites based on double network approach with tailored mechanical, physico-chemical, and biological features.
30208257	1	88	theme	important	278:286	arg1	role					288:291	an important role	275:291	an important role	275:291	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	7	89	from	expression	1476:1485	arg1	medium					1530:1535	basal medium	1524:1535	basal medium	1524:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	7	90	theme	early	1449:1453	arg1	phosphatase					1464:1474	an early alkaline phosphatase	1446:1474	an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium	1446:1535	The results highlighted that composite-DN hydrogels showed a 10-time increase of the storage modulus, if compared to neat MeHA, and an early alkaline phosphatase expression from human mesenchymal stem cells in basal medium.
30208257	5	91	theme	MeHA	933:936	arg1	use					926:928	the synergic use	913:928	the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA)	913:980	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	4	92	theme	reaction	736:743	arg1	parameters					745:754	the chemical reaction parameters	723:754	the chemical reaction parameters	723:754	By fine tuning the DS, the chemical reaction parameters, and the polymer concentration, it was demonstrated the possibility to tailor their mechanical features.
30208257	2	93	theme	photo-cross-linkable	378:397	arg1	motifs					399:404	photo-cross-linkable motifs	378:404	photo-cross-linkable motifs	378:404	In this study, HA was chemically functionalized with photo-cross-linkable motifs by reacting with methacrylic anhydride (MA) to obtain methacrylated hyaluronic acid (MeHA).
30208257	5	94	theme	polyethylene	942:953	arg1	PEGDA					975:979	PEGDA	975:979	PEGDA	975:979	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	5	94	theme	polyethylene	942:953	arg1	diacrylate					963:972	polyethylene glycole diacrylate	942:972	polyethylene glycole diacrylate (PEGDA)	942:980	Double network (DN) hydrogels were prepared through the synergic use of MeHA and polyethylene glycole diacrylate (PEGDA).
30208257	6	95	theme	solid	1047:1051	arg1	signals					1053:1059	bioactive solid signals	1037:1059	bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1037:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	6	95	theme	solid	1047:1051	arg1	nanoparticles					1084:1096	hydroxyapatite nanoparticles	1069:1096	hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach	1069:1131	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	1	96	theme	key	301:303	arg1	processes					314:322	many key cellular processes	296:322	many key cellular processes	296:322	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
30208257	8	97	theme	systematic	1574:1583	arg1	approach					1585:1592	a first systematic approach	1566:1592	a first systematic approach	1566:1592	This work can be considered a first systematic approach for the designing of photo-cross-linkable hydrogels, based on a combination of natural/synthetic polymers and HAp, that could be applied in three-dimensional additive manufacturing techniques such as stereolithography.
30208257	6	98	theme	DN	1023:1024	arg1	hydrogels					1026:1034	DN hydrogels	1023:1034	DN hydrogels	1023:1034	To improve the biological properties of DN hydrogels, bioactive solid signals such as hydroxyapatite nanoparticles (HAp) prepared by sol-gel approach were used in combination with DN hydrogels to obtain an advanced composite material with dual function in terms of mechanical and biological support for soft/hard tissue formation.
30208257	1	99	theme	engineering	232:242	arg1	applications					244:255	tissue engineering applications	225:255	tissue engineering applications	225:255	Hyaluronic acid (HA)-based hydrogels are one of the most promising naturally derived biomaterials for tissue engineering applications, as they can play an important role in many key cellular processes.
29158242	9	0	theme	ΔhfsF	957:961	arg1	mutant					969:974	the ΔhfsF ΔhfaD mutant	953:974	the ΔhfsF ΔhfaD mutant	953:974	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	10	1	theme	ΔhfaD	1200:1204	arg1	mutant					1206:1211	the ΔhfsF ΔhfaD mutant	1190:1211	the ΔhfsF ΔhfaD mutant	1190:1211	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	17	2	theme	adhesin	2375:2381	arg1	knowledge					2343:2351	our specific knowledge	2330:2351	our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment	2330:2416	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	17	2	theme	adhesin	2375:2381	arg1	knowledge					2434:2442	the general knowledge	2422:2442	the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria	2422:2516	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	11	3	theme	holdfast	1393:1400	arg1	anchoring					1402:1410	holdfast anchoring	1393:1410	holdfast anchoring	1393:1410	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	6	4	theme	polysaccharide	703:716	arg1	flippase					718:725	a bacterial polysaccharide flippase	691:725	a bacterial polysaccharide flippase	691:725	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	6	4	theme	polysaccharide	703:716	arg1	HfsF					667:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	13	5	theme	environmental	1914:1926	arg1	stresses					1928:1935	environmental stresses	1914:1935	environmental stresses	1914:1935	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	2	6	theme	adhesive	315:322	arg1	holdfast					339:346	an adhesive polysaccharide holdfast	312:346	an adhesive polysaccharide holdfast	312:346	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	15	7	theme	polysaccharide	2184:2197	arg1	genes					2199:2203	novel polysaccharide genes	2178:2203	novel polysaccharide genes that affect holdfast anchoring to the cell	2178:2246	Here we identify novel polysaccharide genes that affect holdfast anchoring to the cell.
29158242	8	8	from	deficient	883:891	arg1	adherence					896:904	adherence	896:904	adherence	896:904	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	10	9	from	loss	1166:1169	arg1	mutant					1206:1211	the ΔhfsF ΔhfaD mutant	1190:1211	the ΔhfsF ΔhfaD mutant	1190:1211	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	2	10	theme	exact	357:361	arg1	unknown					394:400	unknown	394:400	unknown	394:400	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	2	10	theme	exact	357:361	arg1	composition					363:373	the exact composition	353:373	the exact composition of the holdfast	353:389	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	16	11	theme	anchor	2289:2294	arg1	HfaB					2304:2307	the holdfast anchor protein HfaB	2276:2307	the holdfast anchor protein HfaB	2276:2307	We identify a new role for the holdfast anchor protein HfaB.
29158242	11	12	theme	rfbB	1314:1317	arg1	Loss					1298:1301	Loss	1298:1301	Loss of wbqV or rfbB	1298:1317	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	9	13	theme	epimerases	1098:1107	arg1	family					1065:1070	a family	1063:1070	a family of nucleoside-diphosphate epimerases	1063:1107	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	14	from	mutations	987:995	arg1	genes					1006:1010	three genes	1000:1010	three genes: wbqV, rfbB, and rmlA	1000:1032	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	14	from	mutations	987:995	arg1	rfbB					1019:1022	rfbB	1019:1022	rfbB	1019:1022	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	14	from	mutations	987:995	arg1	rmlA					1029:1032	rmlA	1029:1032	rmlA	1029:1032	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	14	from	mutations	987:995	arg1	wbqV					1013:1016	wbqV	1013:1016	wbqV	1013:1016	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	12	15	theme	ΔhfaB	1676:1680	arg1	mutant					1682:1687	the ΔhfaB mutant	1672:1687	the ΔhfaB mutant	1672:1687	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	2	16	theme	Caulobacter	268:278	arg1	crescentus					280:289	Caulobacter crescentus	268:289	Caulobacter crescentus	268:289	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	12	17	theme	ΔhfaD	1557:1561	arg1	mutant					1563:1568	a ΔhfaA ΔhfaD mutant	1549:1568	a ΔhfaA ΔhfaD mutant	1549:1568	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	8	18	from	adherence	896:904	arg1	mutant					861:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	8	18	from	adherence	896:904	arg1	deficient					883:891	deficient	883:891	deficient	883:891	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	18	19	theme	secretion	2610:2618	arg1	systems					2620:2626	secretion systems	2610:2626	secretion systems	2610:2626	This work also explores the interactions between different polysaccharide biosynthesis and secretion systems in bacteria.
29158242	8	20	theme	ΔhfsF	842:846	arg1	mutant					861:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	8	20	theme	ΔhfsF	842:846	arg1	deficient					883:891	deficient	883:891	deficient	883:891	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	16	21	theme	holdfast	2280:2287	arg1	HfaB					2304:2307	the holdfast anchor protein HfaB	2276:2307	the holdfast anchor protein HfaB	2276:2307	We identify a new role for the holdfast anchor protein HfaB.
29158242	8	22	theme	double	854:859	arg1	mutant					861:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	8	22	theme	double	854:859	arg1	deficient					883:891	deficient	883:891	deficient	883:891	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	6	23	theme	homolog	659:665	arg1	HfsF					667:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	6	23	theme	homolog	659:665	arg1	flippase					718:725	a bacterial polysaccharide flippase	691:725	a bacterial polysaccharide flippase	691:725	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	7	24	theme	hfsF	731:734	arg1	deletion					736:743	An hfsF deletion	728:743	An hfsF deletion	728:743	An hfsF deletion significantly reduced the amount of holdfast produced per cell and slightly reduced adherence.
29158242	15	25	theme	holdfast	2217:2224	arg1	anchoring					2226:2234	holdfast anchoring	2217:2234	holdfast anchoring to the cell	2217:2246	Here we identify novel polysaccharide genes that affect holdfast anchoring to the cell.
29158242	13	26	theme	holdfast	1743:1750	arg1	anchoring					1752:1760	holdfast anchoring	1743:1760	holdfast anchoring	1743:1760	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	1	27	with	interactions	195:206	arg1	surfaces					237:244	both biotic and abiotic surfaces	213:244	both biotic and abiotic surfaces	213:244	Attachment is essential for microorganisms to establish interactions with both biotic and abiotic surfaces.
29158242	0	28	from	Mutations	0:8	arg1	Genes					40:44	Sugar-Nucleotide Synthesis Genes	13:44	Sugar-Nucleotide Synthesis Genes	13:44	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	11	29	theme	holdfast	1489:1496	arg1	anchoring					1498:1506	holdfast anchoring	1489:1506	holdfast anchoring	1489:1506	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	0	30	theme	Holdfast	114:121	arg1	Mutants					130:136	Holdfast Anchor Mutants	114:136	Holdfast Anchor Mutants	114:136	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	18	31	from	systems	2620:2626	arg1	bacteria					2631:2638	bacteria	2631:2638	bacteria	2631:2638	This work also explores the interactions between different polysaccharide biosynthesis and secretion systems in bacteria.
29158242	11	32	theme	ΔhfaD	1417:1421	arg1	mutant					1423:1428	a ΔhfaD mutant	1415:1428	a ΔhfaD mutant	1415:1428	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	5	33	theme	HfaA	602:605	arg1	Translocation					585:597	Translocation	585:597	Translocation of HfaA and HfaD to the cell surface	585:634	Translocation of HfaA and HfaD to the cell surface requires HfaB.
29158242	14	34	theme	permanent	2011:2019	arg1	attachment					2021:2030	permanent attachment	2011:2030	permanent attachment	2011:2030	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	4	35	theme	anchor	507:512	arg1	mutations					519:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	9	36	theme	nucleoside-diphosphate	1075:1096	arg1	epimerases					1098:1107	nucleoside-diphosphate epimerases	1075:1107	nucleoside-diphosphate epimerases	1075:1107	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	3	37	theme	membrane	458:465	arg1	HfaA					476:479	HfaA	476:479	HfaA	476:479	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	37	theme	membrane	458:465	arg1	proteins					467:474	outer membrane proteins HfaA, HfaB, and HfaD	452:495	outer membrane proteins HfaA, HfaB, and HfaD	452:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	37	theme	membrane	458:465	arg1	HfaB					482:485	HfaB	482:485	HfaB	482:485	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	37	theme	membrane	458:465	arg1	HfaD					492:495	HfaD	492:495	HfaD	492:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	5	38	theme	HfaD	611:614	arg1	Translocation					585:597	Translocation	585:597	Translocation of HfaA and HfaD to the cell surface	585:634	Translocation of HfaA and HfaD to the cell surface requires HfaB.
29158242	10	39	theme	holdfast	1259:1266	arg1	synthesis					1268:1276	holdfast synthesis	1259:1276	holdfast synthesis	1259:1276	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	17	40	theme	Caulobacter	2395:2405	arg1	attachment					2407:2416	Caulobacter attachment	2395:2416	Caulobacter attachment	2395:2416	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	14	41	theme	polysaccharide	2082:2095	arg1	anchoring					2097:2105	polysaccharide anchoring	2082:2105	polysaccharide anchoring	2082:2105	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	16	42	theme	new	2263:2265	arg1	role					2267:2270	a new role	2261:2270	a new role for the holdfast anchor protein HfaB	2261:2307	We identify a new role for the holdfast anchor protein HfaB.
29158242	0	43	theme	Sugar-Nucleotide	13:28	arg1	Genes					40:44	Sugar-Nucleotide Synthesis Genes	13:44	Sugar-Nucleotide Synthesis Genes	13:44	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	6	44	theme	Wzx	655:657	arg1	HfsF					667:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	6	44	theme	Wzx	655:657	arg1	flippase					718:725	a bacterial polysaccharide flippase	691:725	a bacterial polysaccharide flippase	691:725	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	16	45	theme	protein	2296:2302	arg1	HfaB					2304:2307	the holdfast anchor protein HfaB	2276:2307	the holdfast anchor protein HfaB	2276:2307	We identify a new role for the holdfast anchor protein HfaB.
29158242	3	46	dep	proteins	467:474	arg1	HfaA					476:479	HfaA	476:479	HfaA	476:479	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	46	dep	proteins	467:474	arg1	proteins					467:474	outer membrane proteins HfaA, HfaB, and HfaD	452:495	outer membrane proteins HfaA, HfaB, and HfaD	452:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	46	dep	proteins	467:474	arg1	HfaB					482:485	HfaB	482:485	HfaB	482:485	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	46	dep	proteins	467:474	arg1	HfaD					492:495	HfaD	492:495	HfaD	492:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	47	theme	cell	435:438	arg1	envelope					440:447	the cell envelope	431:447	the cell envelope	431:447	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	17	48	theme	general	2426:2432	arg1	knowledge					2434:2442	the general knowledge	2422:2442	the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria	2422:2516	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	9	49	theme	suppressor	909:918	arg1	screen					920:925	A suppressor screen	907:925	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant	907:974	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	0	50	theme	Holdfast	54:61	arg1	Anchoring					78:86	Holdfast Polysaccharide Anchoring	54:86	Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants	54:136	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	9	51	dep	mutations	987:995	arg1	has					1119:1121	has	1119:1121	has similarity to nucleotidyltransferases	1119:1159	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	51	dep	mutations	987:995	arg1	belong					1053:1058	belong	1053:1058	belong to a family of nucleoside-diphosphate epimerases	1053:1107	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	4	52	theme	reduced	561:567	arg1	adherence					574:582	reduced cell adherence	561:582	reduced cell adherence	561:582	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	17	53	theme	specific	2334:2341	arg1	knowledge					2343:2351	our specific knowledge	2330:2351	our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment	2330:2416	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	11	54	theme	anchoring	1498:1506	arg1	restoration					1474:1484	restoration	1474:1484	restoration of holdfast anchoring	1474:1506	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	11	55	theme	ΔhfsF	1350:1354	arg1	mutant					1356:1361	a ΔhfsF mutant	1348:1361	a ΔhfsF mutant	1348:1361	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	17	56	theme	polysaccharide	2360:2373	arg1	adhesin					2375:2381	the polysaccharide adhesin	2356:2381	the polysaccharide adhesin involved in Caulobacter attachment	2356:2416	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	9	57	theme	ΔhfaD	963:967	arg1	mutant					969:974	the ΔhfsF ΔhfaD mutant	953:974	the ΔhfsF ΔhfaD mutant	953:974	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	10	58	theme	ΔhfsF	1194:1198	arg1	mutant					1206:1211	the ΔhfsF ΔhfaD mutant	1190:1211	the ΔhfsF ΔhfaD mutant	1190:1211	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	2	59	theme	polysaccharide	324:337	arg1	holdfast					339:346	an adhesive polysaccharide holdfast	312:346	an adhesive polysaccharide holdfast	312:346	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	15	60	theme	novel	2178:2182	arg1	genes					2199:2203	novel polysaccharide genes	2178:2203	novel polysaccharide genes that affect holdfast anchoring to the cell	2178:2246	Here we identify novel polysaccharide genes that affect holdfast anchoring to the cell.
29158242	1	61	theme	biotic	218:223	arg1	surfaces					237:244	both biotic and abiotic surfaces	213:244	both biotic and abiotic surfaces	213:244	Attachment is essential for microorganisms to establish interactions with both biotic and abiotic surfaces.
29158242	17	62	theme	polysaccharide	2482:2495	arg1	adhesins					2497:2504	polysaccharide adhesins	2482:2504	polysaccharide adhesins	2482:2504	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	13	63	theme	additional	1724:1733	arg1	role					1735:1738	an additional role	1721:1738	an additional role	1721:1738	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	13	64	theme	anchoring.IMPORTANCE	1835:1854	arg1	formation					1864:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	1	65	theme	abiotic	229:235	arg1	surfaces					237:244	both biotic and abiotic surfaces	213:244	both biotic and abiotic surfaces	213:244	Attachment is essential for microorganisms to establish interactions with both biotic and abiotic surfaces.
29158242	2	66	theme	crescentus	280:289	arg1	attachment					254:263	Stable attachment	247:263	Stable attachment of Caulobacter crescentus to surfaces	247:301	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	12	67	theme	mutant	1563:1568	arg1	adherence					1513:1521	adherence	1513:1521	adherence	1513:1521	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	12	67	theme	mutant	1563:1568	arg1	anchoring					1536:1544	holdfast anchoring	1527:1544	holdfast anchoring	1527:1544	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	18	68	theme	polysaccharide	2578:2591	arg1	biosynthesis					2593:2604	different polysaccharide biosynthesis	2568:2604	different polysaccharide biosynthesis	2568:2604	This work also explores the interactions between different polysaccharide biosynthesis and secretion systems in bacteria.
29158242	13	69	theme	increased	1885:1893	arg1	resistance					1895:1904	increased resistance	1885:1904	increased resistance to both environmental stresses and antibiotics	1885:1951	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	17	70	theme	adhesins	2497:2504	arg1	anchoring					2469:2477	anchoring	2469:2477	anchoring	2469:2477	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	17	70	theme	adhesins	2497:2504	arg1	production					2454:2463	production	2454:2463	production	2454:2463	This work increases our specific knowledge of the polysaccharide adhesin involved in Caulobacter attachment and the general knowledge regarding production and anchoring of polysaccharide adhesins by bacteria.
29158242	10	71	theme	holdfast	1221:1228	arg1	shedding					1230:1237	holdfast shedding	1221:1237	holdfast shedding	1221:1237	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	8	72	theme	ΔhfaD	848:852	arg1	mutant					861:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant	840:866	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	8	72	theme	ΔhfaD	848:852	arg1	deficient					883:891	deficient	883:891	deficient	883:891	A ΔhfsF ΔhfaD double mutant was completely deficient in adherence.
29158242	12	73	theme	ΔhfaA	1551:1555	arg1	mutant					1563:1568	a ΔhfaA ΔhfaD mutant	1549:1568	a ΔhfaA ΔhfaD mutant	1549:1568	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	12	74	theme	holdfast	1527:1534	arg1	anchoring					1536:1544	holdfast anchoring	1527:1544	holdfast anchoring	1527:1544	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	18	75	theme	different	2568:2576	arg1	biosynthesis					2593:2604	different polysaccharide biosynthesis	2568:2604	different polysaccharide biosynthesis	2568:2604	This work also explores the interactions between different polysaccharide biosynthesis and secretion systems in bacteria.
29158242	13	76	theme	Biofilm	1856:1862	arg1	formation					1864:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	9	77	dep	genes	1006:1010	arg1	genes					1006:1010	three genes	1000:1010	three genes: wbqV, rfbB, and rmlA	1000:1032	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	77	dep	genes	1006:1010	arg1	rfbB					1019:1022	rfbB	1019:1022	rfbB	1019:1022	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	77	dep	genes	1006:1010	arg1	rmlA					1029:1032	rmlA	1029:1032	rmlA	1029:1032	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	77	dep	genes	1006:1010	arg1	wbqV					1013:1016	wbqV	1013:1016	wbqV	1013:1016	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	4	78	theme	holdfast	539:546	arg1	shedding					548:555	holdfast shedding	539:555	holdfast shedding	539:555	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	14	79	theme	holdfast	2127:2134	arg1	unknown					2152:2158	unknown	2152:2158	unknown	2152:2158	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	14	79	theme	holdfast	2127:2134	arg1	composition					2136:2146	the holdfast composition	2123:2146	the holdfast composition	2123:2146	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	0	80	theme	Anchor	123:128	arg1	Mutants					130:136	Holdfast Anchor Mutants	114:136	Holdfast Anchor Mutants	114:136	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	18	81	from	biosynthesis	2593:2604	arg1	bacteria					2631:2638	bacteria	2631:2638	bacteria	2631:2638	This work also explores the interactions between different polysaccharide biosynthesis and secretion systems in bacteria.
29158242	5	82	theme	cell	623:626	arg1	surface					628:634	the cell surface	619:634	the cell surface	619:634	Translocation of HfaA and HfaD to the cell surface requires HfaB.
29158242	14	83	theme	biofilm	2036:2042	arg1	formation					2044:2052	biofilm formation	2036:2052	biofilm formation	2036:2052	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	2	84	attach	attachment	254:263	arg1	surfaces					294:301	surfaces	294:301	surfaces	294:301	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	2	84	attach	attachment	254:263	arg2	crescentus					280:289	Caulobacter crescentus	268:289	Caulobacter crescentus	268:289	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	4	85	theme	Holdfast	498:505	arg1	mutations					519:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	7	86	theme	reduced	821:827	arg1	adherence					829:837	slightly reduced adherence	812:837	slightly reduced adherence	812:837	An hfsF deletion significantly reduced the amount of holdfast produced per cell and slightly reduced adherence.
29158242	12	87	theme	such	1618:1621	arg1	mutations					1623:1631	such mutations	1618:1631	such mutations	1618:1631	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	4	88	theme	gene	514:517	arg1	mutations					519:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations	498:527	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	11	89	theme	wbqV	1306:1309	arg1	Loss					1298:1301	Loss	1298:1301	Loss of wbqV or rfbB	1298:1317	Loss of wbqV or rfbB did not restore adherence to a ΔhfsF mutant but did restore adherence and holdfast anchoring to a ΔhfaD mutant, confirming that suppression occurs through restoration of holdfast anchoring.
29158242	7	90	theme	holdfast	781:788	arg1	amount					771:776	the amount	767:776	the amount of holdfast produced per cell and slightly reduced adherence	767:837	An hfsF deletion significantly reduced the amount of holdfast produced per cell and slightly reduced adherence.
29158242	7	90	theme	holdfast	781:788	arg1	holdfast					781:788	holdfast	781:788	holdfast	781:788	An hfsF deletion significantly reduced the amount of holdfast produced per cell and slightly reduced adherence.
29158242	13	91	theme	holdfast	1826:1833	arg1	formation					1864:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	holdfast anchoring.IMPORTANCE Biofilm formation	1826:1872	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	0	92	dep	crescentus	103:112	arg1	Mutants					130:136	Holdfast Anchor Mutants	114:136	Holdfast Anchor Mutants	114:136	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	14	93	theme	anchoring	2097:2105	arg1	mechanism					2069:2077	the exact mechanism	2059:2077	the exact mechanism of polysaccharide anchoring to the cell	2059:2117	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	14	93	theme	anchoring	2097:2105	arg1	unknown					2152:2158	unknown	2152:2158	unknown	2152:2158	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	14	93	theme	anchoring	2097:2105	arg1	composition					2136:2146	the holdfast composition	2123:2146	the holdfast composition	2123:2146	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	6	94	theme	bacterial	693:701	arg1	flippase					718:725	a bacterial polysaccharide flippase	691:725	a bacterial polysaccharide flippase	691:725	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	6	94	theme	bacterial	693:701	arg1	HfsF					667:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF	651:670	The Wzx homolog HfsF is predicted to be a bacterial polysaccharide flippase.
29158242	0	95	theme	Synthesis	30:38	arg1	Genes					40:44	Sugar-Nucleotide Synthesis Genes	13:44	Sugar-Nucleotide Synthesis Genes	13:44	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	14	96	theme	adhesive	1989:1996	arg1	holdfast					1998:2005	an adhesive holdfast	1986:2005	an adhesive holdfast for permanent attachment and biofilm formation	1986:2052	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	12	97	theme	rfbB	1599:1602	arg1	mutation					1604:1611	rfbB mutation	1599:1611	rfbB mutation	1599:1611	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	10	98	theme	parental	1281:1288	arg1	levels					1290:1295	parental levels	1281:1295	parental levels	1281:1295	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	9	99	contain	has	1119:1121	arg2	similarity					1123:1132	similarity	1123:1132	similarity to nucleotidyltransferases	1123:1159	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	9	99	contain	has	1119:1121	arg1	RmlA					1114:1117	RmlA	1114:1117	RmlA	1114:1117	A suppressor screen that restored adhesion in the ΔhfsF ΔhfaD mutant identified mutations in three genes: wbqV, rfbB, and rmlA Both WbqV and RfbB belong to a family of nucleoside-diphosphate epimerases, and RmlA has similarity to nucleotidyltransferases.
29158242	3	100	theme	outer	452:456	arg1	HfaA					476:479	HfaA	476:479	HfaA	476:479	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	100	theme	outer	452:456	arg1	proteins					467:474	outer membrane proteins HfaA, HfaB, and HfaD	452:495	outer membrane proteins HfaA, HfaB, and HfaD	452:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	100	theme	outer	452:456	arg1	HfaB					482:485	HfaB	482:485	HfaB	482:485	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	3	100	theme	outer	452:456	arg1	HfaD					492:495	HfaD	492:495	HfaD	492:495	The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD.
29158242	14	101	theme	exact	2063:2067	arg1	mechanism					2069:2077	the exact mechanism	2059:2077	the exact mechanism of polysaccharide anchoring to the cell	2059:2117	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	14	101	theme	exact	2063:2067	arg1	unknown					2152:2158	unknown	2152:2158	unknown	2152:2158	Caulobacter crescentus requires an adhesive holdfast for permanent attachment and biofilm formation, but the exact mechanism of polysaccharide anchoring to the cell and the holdfast composition are unknown.
29158242	12	102	dep	adherence	1513:1521	arg1	The					1509:1511	The	1509:1511	The	1509:1511	The adherence and holdfast anchoring of a ΔhfaA ΔhfaD mutant could be restored by wbqV or rfbB mutation, but such mutations could not suppress these phenotypes in the ΔhfaB mutant.
29158242	0	103	theme	Polysaccharide	63:76	arg1	Anchoring					78:86	Holdfast Polysaccharide Anchoring	54:86	Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants	54:136	Mutations in Sugar-Nucleotide Synthesis Genes Restore Holdfast Polysaccharide Anchoring to Caulobacter crescentus Holdfast Anchor Mutants.
29158242	13	104	theme	unknown	1789:1795	arg1	factor					1797:1802	an unknown factor	1786:1802	an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics	1786:1951	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	13	104	theme	unknown	1789:1795	arg1	important					1812:1820	important	1812:1820	important	1812:1820	We hypothesize that HfaB plays an additional role in holdfast anchoring or helps to translocate an unknown factor that is important for holdfast anchoring.IMPORTANCE Biofilm formation results in increased resistance to both environmental stresses and antibiotics.
29158242	4	105	theme	cell	569:572	arg1	adherence					574:582	reduced cell adherence	561:582	reduced cell adherence	561:582	Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence.
29158242	2	106	theme	Stable	247:252	arg1	attachment					254:263	Stable attachment	247:263	Stable attachment of Caulobacter crescentus to surfaces	247:301	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	2	107	theme	holdfast	382:389	arg1	unknown					394:400	unknown	394:400	unknown	394:400	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	2	107	theme	holdfast	382:389	arg1	composition					363:373	the exact composition	353:373	the exact composition of the holdfast	353:389	Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown.
29158242	10	108	theme	rfbB	1182:1185	arg1	loss					1166:1169	The loss	1162:1169	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant	1162:1211	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29158242	10	109	theme	wbqV	1174:1177	arg1	loss					1166:1169	The loss	1162:1169	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant	1162:1211	The loss of wbqV or rfbB in the ΔhfsF ΔhfaD mutant reduced holdfast shedding but did not restore holdfast synthesis to parental levels.
29670011	2	0	gly	glycoprotein	294:305	arg1	glycoprotein					294:305	the glycoprotein N-glycan	290:314	the glycoprotein N-glycan in plants	290:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	5	1	theme	desorption/ionization	763:783	arg1	analyses					800:807	Matrix-assisted laser desorption/ionization time-of-flight analyses	741:807	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans	741:824	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	7	2	theme	Transcriptome	1001:1013	arg1	profiling					1015:1023	Transcriptome profiling	1001:1023	Transcriptome profiling	1001:1023	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	5	3	theme	time-of-flight	785:798	arg1	analyses					800:807	Matrix-assisted laser desorption/ionization time-of-flight analyses	741:807	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans	741:824	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	1	4	theme	key	144:146	arg1	modifications					167:179	the key post-translational modifications	140:179	the key post-translational modifications	140:179	N-linked glycosylation is one of the key post-translational modifications.
29670011	5	5	dep	revealed	826:833	arg1	&alpha					840:845	&alpha	840:845	&alpha	840:845	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	3	6	theme	field	563:567	arg1	conditions					569:578	field conditions	563:578	field conditions	563:578	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	2	7	link	1,3-linked	260:269	arg1	residues					278:285	1,3-linked fucose residues	260:285	&alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants	253:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	0	8	theme	Pollen	81:86	arg1	Viability					88:96	Pollen Viability	81:96	Pollen Viability	81:96	Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice.
29670011	6	9	theme	Mutant	911:916	arg1	complementation					918:932	Mutant complementation	911:932	Mutant complementation	911:932	Mutant complementation revealed that the phenotype was caused by loss of Osfuct function.
29670011	7	10	theme	protein	1152:1158	arg1	kinases					1160:1166	protein kinases	1152:1166	protein kinases	1152:1166	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	8	11	theme	increased	1334:1342	arg1	concentration					1344:1356	an increased concentration	1331:1356	an increased concentration of salt	1331:1364	Moreover, the mutant exhibited sensitivity to an increased concentration of salt.
29670011	3	12	theme	Osfuct	347:352	arg1	mutant					354:359	an Osfuct mutant	344:359	an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions	344:578	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	13	theme	panicle	527:533	arg1	length					535:540	shorter panicle length	519:540	shorter panicle length	519:540	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	1	14	theme	post-translational	148:165	arg1	modifications					167:179	the key post-translational modifications	140:179	the key post-translational modifications	140:179	N-linked glycosylation is one of the key post-translational modifications.
29670011	2	15	dep	&alpha	253:258	arg1	residues					278:285	1,3-linked fucose residues	260:285	&alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants	253:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	2	16	theme	fucose	271:276	arg1	residues					278:285	1,3-linked fucose residues	260:285	&alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants	253:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	1	17	theme	modifications	167:179	arg1	modifications					167:179	the key post-translational modifications	140:179	the key post-translational modifications	140:179	N-linked glycosylation is one of the key post-translational modifications.
29670011	1	17	theme	modifications	167:179	arg1	one					133:135	one	133:135	one	133:135	N-linked glycosylation is one of the key post-translational modifications.
29670011	9	18	theme	further	1392:1398	arg1	understanding					1400:1412	a further understanding	1390:1412	a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice	1390:1511	This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
29670011	5	19	theme	Matrix-assisted	741:755	arg1	desorption/ionization					763:783	Matrix-assisted laser desorption/ionization	741:783	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans	741:824	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	4	20	theme	pollen	657:662	arg1	viability					664:672	pollen viability	657:672	pollen viability	657:672	In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant.
29670011	6	21	theme	function	991:998	arg1	loss					976:979	loss	976:979	loss of Osfuct function	976:998	Mutant complementation revealed that the phenotype was caused by loss of Osfuct function.
29670011	3	22	theme	pollen	439:444	arg1	development					446:456	impaired anther and pollen development	419:456	development	446:456	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	defects					402:408	pleiotropic developmental defects	376:408	pleiotropic developmental defects	376:408	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	development					446:456	impaired anther and pollen development	419:456	development	446:456	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	height					492:497	shorter plant height	478:497	shorter plant height	478:497	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	length					535:540	shorter panicle length	519:540	shorter panicle length	519:540	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	anther					428:433	impaired anther and pollen development	419:456	anther	428:433	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	growth					470:475	diminished growth	459:475	diminished growth	459:475	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	23	theme	pleiotropic	376:386	arg1	tillers					506:512	fewer tillers	500:512	fewer tillers	500:512	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	6	24	theme	Osfuct	984:989	arg1	function					991:998	Osfuct function	984:998	Osfuct function	984:998	Mutant complementation revealed that the phenotype was caused by loss of Osfuct function.
29670011	9	25	theme	pollen	1486:1491	arg1	development					1493:1503	N-glycan modification and anther and pollen development	1449:1503	development	1493:1503	This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
29670011	4	26	theme	pollen	623:628	arg1	grains					630:635	the pollen grains	619:635	the pollen grains	619:635	In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant.
29670011	3	27	theme	developmental	388:400	arg1	defects					402:408	pleiotropic developmental defects	376:408	pleiotropic developmental defects	376:408	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	development					446:456	impaired anther and pollen development	419:456	development	446:456	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	height					492:497	shorter plant height	478:497	shorter plant height	478:497	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	length					535:540	shorter panicle length	519:540	shorter panicle length	519:540	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	anther					428:433	impaired anther and pollen development	419:456	anther	428:433	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	growth					470:475	diminished growth	459:475	diminished growth	459:475	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	27	theme	developmental	388:400	arg1	tillers					506:512	fewer tillers	500:512	fewer tillers	500:512	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	2	28	theme	glycoprotein	294:305	arg1	N-glycan					307:314	the glycoprotein N-glycan	290:314	the glycoprotein N-glycan in plants	290:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	3	29	theme	shorter	478:484	arg1	height					492:497	shorter plant height	478:497	shorter plant height	478:497	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	30	theme	impaired	419:426	arg1	anther					428:433	impaired anther and pollen development	419:456	anther	428:433	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	31	theme	fewer	500:504	arg1	tillers					506:512	fewer tillers	500:512	fewer tillers	500:512	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	3	32	theme	diminished	459:468	arg1	growth					470:475	diminished growth	459:475	diminished growth	459:475	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	0	33	theme	Gene	36:39	arg1	Lack					0:3	Lack	0:3	Lack of the α1,3-Fucosyltransferase Gene (Osfuct)	0:48	Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice.
29670011	0	34	theme	α1,3-Fucosyltransferase	12:34	arg1	Osfuct					42:47	Osfuct	42:47	Osfuct	42:47	Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice.
29670011	0	34	theme	α1,3-Fucosyltransferase	12:34	arg1	Gene					36:39	the α1,3-Fucosyltransferase Gene	8:39	the α1,3-Fucosyltransferase Gene (Osfuct)	8:48	Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice.
29670011	7	35	theme	several	1042:1048	arg1	genes					1050:1054	several genes	1042:1054	several genes essential for plant developmental processes	1042:1098	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	7	36	theme	related	1228:1234	arg1	genes					1222:1226	genes	1222:1226	genes related to protein synthesis	1222:1255	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	7	37	theme	transcription	1169:1181	arg1	factors					1183:1189	transcription factors	1169:1189	transcription factors	1169:1189	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	7	38	theme	plant	1070:1074	arg1	processes					1090:1098	plant developmental processes	1070:1098	plant developmental processes	1070:1098	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	7	39	theme	hypothetical	1262:1273	arg1	proteins					1275:1282	hypothetical proteins	1262:1282	hypothetical proteins	1262:1282	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	5	40	theme	N-glycans	816:824	arg1	analyses					800:807	Matrix-assisted laser desorption/ionization time-of-flight analyses	741:807	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans	741:824	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	7	41	theme	developmental	1076:1088	arg1	processes					1090:1098	plant developmental processes	1070:1098	plant developmental processes	1070:1098	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	1	42	theme	N-linked	107:114	arg1	glycosylation					116:128	N-linked glycosylation	107:128	N-linked glycosylation	107:128	N-linked glycosylation is one of the key post-translational modifications.
29670011	9	43	theme	function	1421:1428	arg1	understanding					1400:1412	a further understanding	1390:1412	a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice	1390:1511	This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
29670011	7	44	theme	essential	1056:1064	arg1	genes					1050:1054	several genes	1042:1054	several genes essential for plant developmental processes	1042:1098	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
29670011	2	45	theme	1,3-linked	260:269	arg1	residues					278:285	1,3-linked fucose residues	260:285	&alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants	253:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	3	46	theme	plant	486:490	arg1	height					492:497	shorter plant height	478:497	shorter plant height	478:497	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	4	47	theme	pollen	678:683	arg1	number					685:690	pollen number	678:690	pollen number per anther	678:701	In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant.
29670011	1	48	link	N-linked	107:114	arg1	glycosylation					116:128	N-linked glycosylation	107:128	N-linked glycosylation	107:128	N-linked glycosylation is one of the key post-translational modifications.
29670011	5	49	theme	mutant	903:908	arg1	structure					886:894	the N-glycan structure	873:894	the N-glycan structure of the mutant	873:908	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	9	50	theme	genes	1433:1437	arg1	function					1421:1428	the function	1417:1428	the function of genes mediating N-glycan modification and anther and pollen development in rice	1417:1511	This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
29670011	0	51	theme	Anther	58:63	arg1	Development					65:75	Anther Development	58:75	Anther Development	58:75	Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice.
29670011	8	52	theme	salt	1361:1364	arg1	concentration					1344:1356	an increased concentration	1331:1356	an increased concentration of salt	1331:1364	Moreover, the mutant exhibited sensitivity to an increased concentration of salt.
29670011	5	53	theme	N-glycan	877:884	arg1	structure					886:894	the N-glycan structure	873:894	the N-glycan structure of the mutant	873:908	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	5	54	theme	laser	757:761	arg1	desorption/ionization					763:783	Matrix-assisted laser desorption/ionization	741:783	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans	741:824	Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that &alpha;1,3-fucose was lacking in the N-glycan structure of the mutant.
29670011	9	55	theme	N-glycan	1449:1456	arg1	modification					1458:1469	N-glycan modification and anther and pollen development	1449:1503	modification	1458:1469	This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
29670011	3	56	theme	shorter	519:525	arg1	length					535:540	shorter panicle length	519:540	shorter panicle length	519:540	We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions.
29670011	2	57	from	N-glycan	307:314	arg1	plants					319:324	plants	319:324	plants	319:324	&alpha;1,3-Fucosyltransferase (OsFucT) is responsible for transferring &alpha;1,3-linked fucose residues to the glycoprotein N-glycan in plants.
29670011	7	58	theme	protein	1239:1245	arg1	synthesis					1247:1255	protein synthesis	1239:1255	protein synthesis	1239:1255	Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins.
30451443	8	0	theme	adhesion	1298:1305	arg1	efficiency					1307:1316	adhesion efficiency	1298:1316	adhesion efficiency of L. rhamnosus KL 53A	1298:1339	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	6	1	dep	Blautia	1091:1097	arg1	luti					1099:1102	luti	1099:1102	luti	1099:1102	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	2	theme	selected	975:982	arg1	strains					1006:1012	the selected intestinal microbiota strains	971:1012	the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	971:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	3	theme	microbiota	995:1004	arg1	strains					1006:1012	the selected intestinal microbiota strains	971:1012	the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	971:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	8	4	theme	L.	1321:1322	arg1	53A					1337:1339	L. rhamnosus KL 53A	1321:1339	L. rhamnosus KL 53A	1321:1339	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	1	5	theme	beneficial	169:178	arg1	composition					191:201	beneficial microbiota composition	169:201	beneficial microbiota composition of the gastrointestinal tract ecosystem	169:241	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	8	6	theme	strains	1387:1393	arg1	co-incubation					1351:1363	co-incubation	1351:1363	co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677	1351:1423	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	6	7	theme	DSM	1104:1106	arg1	14534					1108:1112	Blautia luti DSM 14534	1091:1112	Blautia luti DSM 14534	1091:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	3	8	from	tract	442:446	arg1	chance					393:398	the chance	389:398	the chance of their survival in the gastrointestinal tract	389:446	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	7	9	with	deglycosylation	1120:1134	arg1	strains					1184:1190	polysaccharide metabolizing strains	1156:1190	polysaccharide metabolizing strains	1156:1190	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	3	10	from	chance	393:398	arg1	tract					442:446	the gastrointestinal tract	421:446	the gastrointestinal tract	421:446	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	6	11	gly	deglycosylation	912:926	arg1	cells					945:949	the bacterial cells	931:949	the bacterial cells	931:949	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	12	theme	Blautia	1091:1097	arg1	14534					1108:1112	Blautia luti DSM 14534	1091:1112	Blautia luti DSM 14534	1091:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	8	13	theme	rhamnosus	1324:1332	arg1	53A					1337:1339	L. rhamnosus KL 53A	1321:1339	L. rhamnosus KL 53A	1321:1339	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	7	14	theme	strains	1228:1234	arg1	ability					1207:1213	the ability	1203:1213	the ability of probiotic strains to adhere to enterocytes	1203:1259	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	8	15	theme	prausnitzii	1403:1413	arg1	17677					1419:1423	F. prausnitzii DSM 17677	1400:1423	F. prausnitzii DSM 17677	1400:1423	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	3	16	theme	colonization	552:563	arg1	process					541:547	the process	537:547	the process of colonization	537:563	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	1	17	theme	microbiota	180:189	arg1	composition					191:201	beneficial microbiota composition	169:201	beneficial microbiota composition of the gastrointestinal tract ecosystem	169:241	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	5	18	theme	liquid	795:800	arg1	chromatography					802:815	liquid chromatography	795:815	liquid chromatography	795:815	Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography.
30451443	3	19	theme	gastrointestinal	425:440	arg1	tract					442:446	the gastrointestinal tract	421:446	the gastrointestinal tract	421:446	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	6	20	theme	lactobacilli	857:868	arg1	adhesion					870:877	lactobacilli adhesion	857:877	lactobacilli adhesion to enterocytes	857:892	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	8	21	theme	KL	1334:1335	arg1	53A					1337:1339	L. rhamnosus KL 53A	1321:1339	L. rhamnosus KL 53A	1321:1339	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	9	22	theme	spp	1538:1540	arg1	Exopolysaccharides					1479:1496	Exopolysaccharides	1479:1496	Exopolysaccharides	1479:1496	Exopolysaccharides are important adhesins of Lactobacillus spp.
30451443	9	22	theme	spp	1538:1540	arg1	adhesins					1512:1519	important adhesins	1502:1519	important adhesins of Lactobacillus spp	1502:1540	Exopolysaccharides are important adhesins of Lactobacillus spp.
30451443	8	23	theme	lactobacillus	1373:1385	arg1	strains					1387:1393	both lactobacillus strains	1368:1393	both lactobacillus strains	1368:1393	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	8	24	theme	53A	1337:1339	arg1	efficiency					1307:1316	adhesion efficiency	1298:1316	adhesion efficiency of L. rhamnosus KL 53A	1298:1339	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	3	25	from	survival	409:416	arg1	tract					442:446	the gastrointestinal tract	421:446	the gastrointestinal tract	421:446	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	10	26	theme	gut	1584:1586	arg1	epithelium					1588:1597	gut epithelium	1584:1597	gut epithelium	1584:1597	that influence their ability to colonize gut epithelium.
30451443	2	27	theme	important	331:339	arg1	Production					292:301	Production	292:301	Production of exopolysaccharides	292:323	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	2	27	theme	important	331:339	arg1	feature					341:347	an important feature	328:347	an important feature of probiotic lactobacilli	328:373	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	7	28	theme	metabolizing	1171:1182	arg1	strains					1184:1190	polysaccharide metabolizing strains	1156:1190	polysaccharide metabolizing strains	1156:1190	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	8	29	theme	F.	1400:1401	arg1	17677					1419:1423	F. prausnitzii DSM 17677	1400:1423	F. prausnitzii DSM 17677	1400:1423	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	3	30	theme	survival	409:416	arg1	chance					393:398	the chance	389:398	the chance of their survival in the gastrointestinal tract	389:446	It increases the chance of their survival in the gastrointestinal tract and promotes adhesion to the epithelium; therefore, exopolysaccharides are important for the process of colonization.
30451443	4	31	theme	bacteria	582:589	arg1	strains					591:597	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	7	32	theme	probiotic	1218:1226	arg1	strains					1228:1234	probiotic strains	1218:1234	probiotic strains	1218:1234	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	6	33	theme	exopolysaccharides	835:852	arg1	influence					822:830	The influence	818:830	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes	818:892	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	4	34	theme	acid	577:580	arg1	bacteria					582:589	Two lactic acid bacteria	566:589	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	11	35	theme	commensal	1724:1732	arg1	bacteria					1734:1741	commensal bacteria	1724:1741	commensal bacteria	1724:1741	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	8	36	theme	efficiency	1467:1476	arg1	increase					1440:1447	an increase	1437:1447	an increase of their adhesion efficiency	1437:1476	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	5	37	attach	isolated	709:716	arg2	Exopolysaccharides					685:702	Exopolysaccharides	685:702	Exopolysaccharides	685:702	Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography.
30451443	5	37	attach	isolated	709:716	arg1	cells					733:737	bacterial cells	723:737	bacterial cells	723:737	Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography.
30451443	1	38	theme	gastrointestinal	210:225	arg1	tract					227:231	the gastrointestinal tract ecosystem	206:241	the gastrointestinal tract ecosystem	206:241	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	6	39	theme	DSM	1077:1079	arg1	17677					1081:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	40	dep	polysaccharides	1030:1044	arg1	14534					1108:1112	Blautia luti DSM 14534	1091:1112	Blautia luti DSM 14534	1091:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	40	dep	polysaccharides	1030:1044	arg1	17677					1081:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	2	41	theme	exopolysaccharides	306:323	arg1	Production					292:301	Production	292:301	Production of exopolysaccharides	292:323	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	2	41	theme	exopolysaccharides	306:323	arg1	feature					341:347	an important feature	328:347	an important feature of probiotic lactobacilli	328:373	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	11	42	theme	gut	1617:1619	arg1	microbiota					1621:1630	gut microbiota	1617:1630	gut microbiota	1617:1630	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	1	43	theme	tract	227:231	arg1	composition					191:201	beneficial microbiota composition	169:201	beneficial microbiota composition of the gastrointestinal tract ecosystem	169:241	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	0	44	theme	KL	55:56	arg1	53A					58:60	KL 53A	55:60	Lactobacillus rhamnosus KL 53A	31:60	Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes.
30451443	5	45	theme	monosaccharide	749:762	arg1	composition					764:774	their monosaccharide composition	743:774	their monosaccharide composition	743:774	Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography.
30451443	6	46	theme	cells	945:949	arg1	deglycosylation					912:926	deglycosylation	912:926	deglycosylation of the bacterial cells	912:949	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	6	46	theme	cells	945:949	arg1	incubation					955:964	incubation	955:964	incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	955:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	11	47	theme	microbiota	1621:1630	arg1	members					1606:1612	Other members	1600:1612	Other members of gut microbiota	1600:1630	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	9	48	theme	important	1502:1510	arg1	Exopolysaccharides					1479:1496	Exopolysaccharides	1479:1496	Exopolysaccharides	1479:1496	Exopolysaccharides are important adhesins of Lactobacillus spp.
30451443	9	48	theme	important	1502:1510	arg1	adhesins					1512:1519	important adhesins	1502:1519	important adhesins of Lactobacillus spp	1502:1540	Exopolysaccharides are important adhesins of Lactobacillus spp.
30451443	7	49	with	incubation	1140:1149	arg1	strains					1184:1190	polysaccharide metabolizing strains	1156:1190	polysaccharide metabolizing strains	1156:1190	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	6	50	theme	Faecalibacterium	1048:1063	arg1	17677					1081:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	Faecalibacterium prausnitzii DSM 17677	1048:1085	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	11	51	theme	metabolic	1705:1713	arg1	state					1715:1719	metabolic state	1705:1719	metabolic state	1705:1719	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	6	52	theme	bacterial	935:943	arg1	cells					945:949	the bacterial cells	931:949	the bacterial cells	931:949	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	1	53	theme	positive	254:261	arg1	impact					263:268	a positive impact	252:268	a positive impact on the host's health	252:289	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	11	54	theme	probiotic	1763:1771	arg1	action					1773:1778	their probiotic action	1757:1778	their probiotic action	1757:1778	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	6	55	with	deglycosylation	912:926	arg1	strains					1006:1012	the selected intestinal microbiota strains	971:1012	the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	971:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	9	56	theme	Lactobacillus	1524:1536	arg1	spp					1538:1540	Lactobacillus spp	1524:1540	Lactobacillus spp	1524:1540	Exopolysaccharides are important adhesins of Lactobacillus spp.
30451443	7	57	theme	polysaccharide	1156:1169	arg1	strains					1184:1190	polysaccharide metabolizing strains	1156:1190	polysaccharide metabolizing strains	1156:1190	Both deglycosylation and incubation with polysaccharide metabolizing strains influenced the ability of probiotic strains to adhere to enterocytes.
30451443	5	58	theme	bacterial	723:731	arg1	cells					733:737	bacterial cells	723:737	bacterial cells	723:737	Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography.
30451443	2	59	theme	lactobacilli	362:373	arg1	Production					292:301	Production	292:301	Production of exopolysaccharides	292:323	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	2	59	theme	lactobacilli	362:373	arg1	feature					341:347	an important feature	328:347	an important feature of probiotic lactobacilli	328:373	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	6	60	theme	intestinal	984:993	arg1	strains					1006:1012	the selected intestinal microbiota strains	971:1012	the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	971:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	4	61	theme	lactic	570:575	arg1	bacteria					582:589	Two lactic acid bacteria	566:589	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	1	62	from	impact	263:268	arg1	health					284:289	the host's health	273:289	the host's health	273:289	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	2	63	theme	probiotic	352:360	arg1	lactobacilli					362:373	probiotic lactobacilli	352:373	probiotic lactobacilli	352:373	Production of exopolysaccharides is an important feature of probiotic lactobacilli.
30451443	11	64	dep	composition	1689:1699	arg1	the					1685:1687	the	1685:1687	the	1685:1687	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	4	65	used	used	604:607	arg2	Lactobacillus					624:636	Lactobacillus	624:636	Lactobacillus	624:636	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	4	65	used	used	604:607	arg2	strains					591:597	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains	566:597	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	4	65	used	used	604:607	arg2	casei					673:677	Lactobacillus casei	659:677	Lactobacillus casei	659:677	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	11	66	theme	bacteria	1734:1741	arg1	composition					1689:1699	composition	1689:1699	composition	1689:1699	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	11	66	theme	bacteria	1734:1741	arg1	state					1715:1719	metabolic state	1705:1719	metabolic state	1705:1719	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	0	67	dep	Lactobacillus	31:43	arg1	Fyos					86:89	Fyos	86:89	Fyos	86:89	Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes.
30451443	0	67	dep	Lactobacillus	31:43	arg1	53A					58:60	KL 53A	55:60	Lactobacillus rhamnosus KL 53A	31:60	Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes.
30451443	0	67	dep	Lactobacillus	31:43	arg1	rhamnosus					45:53	Lactobacillus rhamnosus KL 53A	31:60	Lactobacillus rhamnosus KL 53A	31:60	Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes.
30451443	11	68	theme	Other	1600:1604	arg1	members					1606:1612	Other members	1600:1612	Other members of gut microbiota	1600:1630	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	6	69	from	influence	822:830	arg1	adhesion					870:877	lactobacilli adhesion	857:877	lactobacilli adhesion to enterocytes	857:892	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	4	70	dep	Lactobacillus	624:636	arg1	53A					651:653	KL 53A	648:653	Lactobacillus rhamnosus KL 53A	624:653	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	4	70	dep	Lactobacillus	624:636	arg1	rhamnosus					638:646	Lactobacillus rhamnosus KL 53A	624:653	Lactobacillus rhamnosus KL 53A	624:653	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	4	70	dep	Lactobacillus	624:636	arg1	Fyos					679:682	Fyos	679:682	Fyos	679:682	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	6	71	dep	Faecalibacterium	1048:1063	arg1	prausnitzii					1065:1075	prausnitzii	1065:1075	prausnitzii	1065:1075	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30451443	8	72	theme	DSM	1415:1417	arg1	17677					1419:1423	F. prausnitzii DSM 17677	1400:1423	F. prausnitzii DSM 17677	1400:1423	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	8	73	theme	adhesion	1458:1465	arg1	efficiency					1467:1476	their adhesion efficiency	1452:1476	their adhesion efficiency	1452:1476	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	11	74	theme	adhesion	1647:1654	arg1	property					1656:1663	the adhesion property	1643:1663	the adhesion property	1643:1663	Other members of gut microbiota can modify the adhesion property in situ ; therefore the composition and metabolic state of commensal bacteria may influence their probiotic action.
30451443	4	75	theme	KL	648:649	arg1	53A					651:653	KL 53A	648:653	Lactobacillus rhamnosus KL 53A	624:653	Two lactic acid bacteria strains were used in this study: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos.
30451443	8	76	theme	Enzymatic	1262:1270	arg1	deglycosylation					1272:1286	Enzymatic deglycosylation	1262:1286	Enzymatic deglycosylation	1262:1286	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	1	77	dep	tract	227:231	arg1	ecosystem					233:241	ecosystem	233:241	the gastrointestinal tract ecosystem	206:241	Probiotics promote and help to maintain beneficial microbiota composition of the gastrointestinal tract ecosystem and have a positive impact on the host's health.
30451443	8	78	with	co-incubation	1351:1363	arg1	17677					1419:1423	F. prausnitzii DSM 17677	1400:1423	F. prausnitzii DSM 17677	1400:1423	Enzymatic deglycosylation decreased adhesion efficiency of L. rhamnosus KL 53A; however, co-incubation of both lactobacillus strains with F. prausnitzii DSM 17677 resulted in an increase of their adhesion efficiency.
30451443	6	79	with	incubation	955:964	arg1	strains					1006:1012	the selected intestinal microbiota strains	971:1012	the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534	971:1112	The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides - Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534.
30099038	0	0	theme	cerebral	70:77	arg1	cortex					79:84	the mouse cerebral cortex	60:84	the mouse cerebral cortex	60:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	7	1	theme	HABP-positive	885:897	arg1	PNNs					899:902	many HABP-positive PNNs	880:902	many HABP-positive PNNs	880:902	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30099038	1	2	theme	volume	191:196	arg1	%					182:182	more than 20%	170:182	more than 20% of the volume	170:196	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	1	2	theme	volume	191:196	arg1	volume					191:196	the volume	187:196	the volume	187:196	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	0	3	theme	mouse	64:68	arg1	cortex					79:84	the mouse cerebral cortex	60:84	the mouse cerebral cortex	60:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	10	4	contain	have	1208:1211	arg1	PNNs					1174:1177	HABP-positive PNNs	1160:1177	HABP-positive PNNs in layer 1 of the cortex	1160:1202	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	4	contain	have	1208:1211	arg2	differentiation					1250:1264	neuronal differentiation	1241:1264	neuronal differentiation	1241:1264	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	4	contain	have	1208:1211	arg2	migration					1286:1294	migration	1286:1294	migration	1286:1294	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	4	contain	have	1208:1211	arg2	functions					1223:1231	different functions	1213:1231	different functions such as neuronal differentiation, proliferation, and migration	1213:1294	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	4	contain	have	1208:1211	arg2	proliferation					1267:1279	proliferation	1267:1279	proliferation	1267:1279	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	1	5	theme	extracellular	124:136	arg1	ECM					146:148	ECM	146:148	ECM	146:148	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	1	5	theme	extracellular	124:136	arg1	matrix					138:143	extracellular matrix	124:143	extracellular matrix (ECM) molecules	124:159	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	5	6	theme	cortical	624:631	arg1	distribution					649:660	the cortical region-specific distribution	620:660	the cortical region-specific distribution of HA	620:666	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	6	7	theme	HABP-positive	800:812	arg1	PNNs					814:817	HABP-positive PNNs	800:817	HABP-positive PNNs	800:817	Our findings revealed that HABP-positive PNNs are present throughout the mouse cortex.
30099038	9	8	theme	mouse	1097:1101	arg1	cortex					1103:1108	the mouse cortex	1093:1108	the mouse cortex	1093:1108	These findings indicate that PNNs in the mouse cortex show region-dependent differences in composition.
30099038	9	9	from	PNNs	1085:1088	arg1	cortex					1103:1108	the mouse cortex	1093:1108	the mouse cortex	1093:1108	These findings indicate that PNNs in the mouse cortex show region-dependent differences in composition.
30099038	5	10	theme	region-specific	633:647	arg1	distribution					649:660	the cortical region-specific distribution	620:660	the cortical region-specific distribution of HA	620:666	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	0	11	from	nets	52:55	arg1	cortex					79:84	the mouse cerebral cortex	60:84	the mouse cerebral cortex	60:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	10	12	theme	different	1213:1221	arg1	proliferation					1267:1279	proliferation	1267:1279	proliferation	1267:1279	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	12	theme	different	1213:1221	arg1	migration					1286:1294	migration	1286:1294	migration	1286:1294	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	12	theme	different	1213:1221	arg1	differentiation					1250:1264	neuronal differentiation	1241:1264	neuronal differentiation	1241:1264	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	10	12	theme	different	1213:1221	arg1	functions					1223:1231	different functions	1213:1231	different functions such as neuronal differentiation, proliferation, and migration	1213:1294	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	0	13	attach	present	19:25	arg2	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	0	13	attach	present	19:25	arg1	nets					52:55	specific perineuronal nets	30:55	specific perineuronal nets in the mouse cerebral cortex	30:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	1	14	theme	matrix	138:143	arg1	molecules					151:159	extracellular matrix (ECM) molecules	124:159	extracellular matrix (ECM) molecules	124:159	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	5	15	theme	protein	707:713	arg1	PNNs					731:734	HA binding protein (HABP)-positive PNNs	696:734	HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex	696:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	9	16	theme	region-dependent	1115:1130	arg1	differences					1132:1142	region-dependent differences	1115:1142	region-dependent differences in composition	1115:1157	These findings indicate that PNNs in the mouse cortex show region-dependent differences in composition.
30099038	10	17	from	PNNs	1174:1177	arg1	layer					1182:1186	layer 1	1182:1188	layer 1 of the cortex	1182:1202	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	4	18	theme	HA	566:567	arg1	distribution					550:561	the distribution	546:561	the distribution of HA in the cortex	546:581	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	10	19	theme	cortex	1197:1202	arg1	layer					1182:1186	layer 1	1182:1188	layer 1 of the cortex	1182:1202	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	0	20	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	3	21	from	CNS	410:412	arg1	components					381:390	The components	377:390	The components of the ECM in the CNS	377:412	The components of the ECM in the CNS include proteins, proteoglycans, and glycosaminoglycans.
30099038	10	22	theme	neuronal	1241:1248	arg1	differentiation					1250:1264	neuronal differentiation	1241:1264	neuronal differentiation	1241:1264	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	2	23	theme	ECM	312:314	arg1	nets					296:299	Perineuronal nets	283:299	Perineuronal nets (PNNs)	283:306	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	2	23	theme	ECM	312:314	arg1	molecules					316:324	ECM molecules	312:324	ECM molecules that highly accumulate around the soma of neurons	312:374	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	2	24	theme	neurons	368:374	arg1	soma					360:363	the soma	356:363	the soma of neurons	356:374	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	1	25	theme	neuronal	218:225	arg1	plasticity					227:236	neuronal plasticity	218:236	neuronal plasticity	218:236	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	8	26	theme	HABP-positive	1011:1023	arg1	PNNs					1025:1028	granular-like HABP-positive PNNs	997:1028	granular-like HABP-positive PNNs	997:1028	Furthermore, we observed granular-like HABP-positive PNNs in layer 1 of the cortex.
30099038	0	27	located	present	19:25	arg2	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	0	27	located	present	19:25	arg1	nets					52:55	specific perineuronal nets	30:55	specific perineuronal nets in the mouse cerebral cortex	30:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	4	28	from	distribution	550:561	arg1	cortex					576:581	the cortex	572:581	the cortex	572:581	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	5	29	theme	-positive	721:729	arg1	PNNs					731:734	HA binding protein (HABP)-positive PNNs	696:734	HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex	696:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	8	30	theme	granular-like	997:1009	arg1	PNNs					1025:1028	granular-like HABP-positive PNNs	997:1028	granular-like HABP-positive PNNs	997:1028	Furthermore, we observed granular-like HABP-positive PNNs in layer 1 of the cortex.
30099038	3	31	theme	ECM	399:401	arg1	components					381:390	The components	377:390	The components of the ECM in the CNS	377:412	The components of the ECM in the CNS include proteins, proteoglycans, and glycosaminoglycans.
30099038	0	32	theme	specific	30:37	arg1	nets					52:55	specific perineuronal nets	30:55	specific perineuronal nets in the mouse cerebral cortex	30:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	2	33	theme	Perineuronal	283:294	arg1	PNNs					302:305	PNNs	302:305	PNNs	302:305	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	2	33	theme	Perineuronal	283:294	arg1	nets					296:299	Perineuronal nets	283:299	Perineuronal nets (PNNs)	283:306	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	2	33	theme	Perineuronal	283:294	arg1	molecules					316:324	ECM molecules	312:324	ECM molecules that highly accumulate around the soma of neurons	312:374	Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons.
30099038	5	34	theme	mouse	750:754	arg1	cortex					765:770	the mature mouse cerebral cortex	739:770	the mature mouse cerebral cortex	739:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	1	35	theme	synaptic	239:246	arg1	transmission					248:259	synaptic transmission	239:259	synaptic transmission	239:259	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	5	36	theme	HA	696:697	arg1	HABP					716:719	HABP	716:719	HABP	716:719	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	5	36	theme	HA	696:697	arg1	protein					707:713	HA binding protein	696:713	HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex	696:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	3	37	from	components	381:390	arg1	CNS					410:412	the CNS	406:412	the CNS	406:412	The components of the ECM in the CNS include proteins, proteoglycans, and glycosaminoglycans.
30099038	5	38	theme	mature	743:748	arg1	cortex					765:770	the mature mouse cerebral cortex	739:770	the mature mouse cerebral cortex	739:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	5	39	theme	binding	699:705	arg1	HABP					716:719	HABP	716:719	HABP	716:719	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	5	39	theme	binding	699:705	arg1	protein					707:713	HA binding protein	696:713	HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex	696:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	6	40	theme	mouse	846:850	arg1	cortex					852:857	the mouse cortex	842:857	the mouse cortex	842:857	Our findings revealed that HABP-positive PNNs are present throughout the mouse cortex.
30099038	4	41	theme	hyaluronic	480:489	arg1	acid					491:494	hyaluronic acid	480:494	hyaluronic acid (HA)	480:499	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	4	41	theme	hyaluronic	480:489	arg1	HA					497:498	HA	497:498	HA	497:498	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	7	42	theme	Wisteria	926:933	arg1	PNNs					966:969	Wisteria floribunda agglutinin-positive PNNs	926:969	Wisteria floribunda agglutinin-positive PNNs	926:969	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30099038	1	43	theme	central	94:100	arg1	system					110:115	the central nervous system	90:115	the central nervous system (CNS)	90:121	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	1	43	theme	central	94:100	arg1	CNS					118:120	CNS	118:120	CNS	118:120	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	0	44	theme	perineuronal	39:50	arg1	nets					52:55	specific perineuronal nets	30:55	specific perineuronal nets in the mouse cerebral cortex	30:84	Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex.
30099038	5	45	from	PNNs	731:734	arg1	cortex					765:770	the mature mouse cerebral cortex	739:770	the mature mouse cerebral cortex	739:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	1	46	theme	nervous	102:108	arg1	system					110:115	the central nervous system	90:115	the central nervous system (CNS)	90:121	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	1	46	theme	nervous	102:108	arg1	CNS					118:120	CNS	118:120	CNS	118:120	In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation.
30099038	7	47	theme	floribunda	935:944	arg1	PNNs					966:969	Wisteria floribunda agglutinin-positive PNNs	926:969	Wisteria floribunda agglutinin-positive PNNs	926:969	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30099038	3	48	from	ECM	399:401	arg1	CNS					410:412	the CNS	406:412	the CNS	406:412	The components of the ECM in the CNS include proteins, proteoglycans, and glycosaminoglycans.
30099038	7	49	theme	agglutinin-positive	946:964	arg1	PNNs					966:969	Wisteria floribunda agglutinin-positive PNNs	926:969	Wisteria floribunda agglutinin-positive PNNs	926:969	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30099038	7	50	theme	PNNs	899:902	arg1	distribution					864:875	The distribution	860:875	The distribution of many HABP-positive PNNs	860:902	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30099038	9	51	from	differences	1132:1142	arg1	composition					1147:1157	composition	1147:1157	composition	1147:1157	These findings indicate that PNNs in the mouse cortex show region-dependent differences in composition.
30099038	10	52	theme	HABP-positive	1160:1172	arg1	PNNs					1174:1177	HABP-positive PNNs	1160:1177	HABP-positive PNNs in layer 1 of the cortex	1160:1202	HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.
30099038	4	53	theme	constituent	517:527	arg1	element					529:535	a constituent element	515:535	a constituent element of PNNs	515:543	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	5	54	theme	HA	665:666	arg1	distribution					649:660	the cortical region-specific distribution	620:660	the cortical region-specific distribution of HA	620:666	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	5	55	theme	cerebral	756:763	arg1	cortex					765:770	the mature mouse cerebral cortex	739:770	the mature mouse cerebral cortex	739:770	To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex.
30099038	4	56	theme	PNNs	540:543	arg1	element					529:535	a constituent element	515:535	a constituent element of PNNs	515:543	Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified.
30099038	8	57	theme	cortex	1048:1053	arg1	layer					1033:1037	layer 1	1033:1039	layer 1 of the cortex	1033:1053	Furthermore, we observed granular-like HABP-positive PNNs in layer 1 of the cortex.
30099038	7	58	theme	many	880:883	arg1	PNNs					899:902	many HABP-positive PNNs	880:902	many HABP-positive PNNs	880:902	The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs.
30784171	5	0	theme	coating	743:749	arg1	effect					751:756	the coating effect	739:756	the coating effect on the hollow structure	739:780	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	1	1	theme	microfiber-based	140:155	arg1	alginate					101:108	Sodium alginate	94:108	Sodium alginate (NaA)	94:114	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	1	theme	microfiber-based	140:155	arg1	material					166:173	microfiber-based scaffold material	140:173	microfiber-based scaffold material	140:173	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	9	2	theme	innovative	1385:1394	arg1	methodologies					1396:1408	innovative methodologies	1385:1408	innovative methodologies	1385:1408	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	2	3	from	application	324:334	arg1	engineering					346:356	tissue engineering	339:356	tissue engineering	339:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	3	4	theme	Ca-alginate	502:512	arg1	microfiber					521:530	Ca-alginate hollow microfiber	502:530	Ca-alginate hollow microfiber	502:530	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	7	5	theme	low	1127:1129	arg1	affinity					1131:1138	the low affinity	1123:1138	the low affinity of alginate to cell surfaces	1123:1167	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	6	6	theme	complex	881:887	arg1	membrane					889:896	a stable polyelectrolyte complex membrane	856:896	a stable polyelectrolyte complex membrane	856:896	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	7	7	theme	chitosan	1072:1079	arg1	coating					1081:1087	chitosan coating	1072:1087	chitosan coating	1072:1087	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	7	8	from	culture	1207:1213	arg1	microfiber					1222:1231	the microfiber	1218:1231	the microfiber	1218:1231	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	3	9	theme	microfluidic	550:561	arg1	device					563:568	a microfluidic device	548:568	a microfluidic device	548:568	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	4	10	theme	composite	585:593	arg1	microfiber					595:604	A more stable composite microfiber	571:604	A more stable composite microfiber with double cross-linking layers	571:637	A more stable composite microfiber with double cross-linking layers was generated.
30784171	6	11	theme	polyelectrolyte	865:879	arg1	complex					881:887	a stable polyelectrolyte complex	856:887	a stable polyelectrolyte complex membrane	856:896	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	7	12	theme	coating	1081:1087	arg1	introduction					1056:1067	the introduction	1052:1067	the introduction of chitosan coating	1052:1087	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	2	13	theme	ions	287:290	arg1	loss					271:274	the loss	267:274	the loss	267:274	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	4	14	theme	stable	578:583	arg1	microfiber					595:604	A more stable composite microfiber	571:604	A more stable composite microfiber with double cross-linking layers	571:637	A more stable composite microfiber with double cross-linking layers was generated.
30784171	2	15	from	disintegrate	214:225	arg1	environment					248:258	the physiological environment	230:258	the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering	230:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	6	16	theme	stable	858:863	arg1	complex					881:887	a stable polyelectrolyte complex	856:887	a stable polyelectrolyte complex membrane	856:896	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	2	17	theme	calcium	279:285	arg1	ions					287:290	calcium ions	279:290	calcium ions	279:290	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	7	18	theme	cell	1155:1158	arg1	surfaces					1160:1167	cell surfaces	1155:1167	cell surfaces	1155:1167	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	1	19	used	used	132:135	arg2	NaA					111:113	NaA	111:113	NaA	111:113	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	19	used	used	132:135	arg2	material					166:173	microfiber-based scaffold material	140:173	microfiber-based scaffold material	140:173	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	19	used	used	132:135	arg2	alginate					101:108	Sodium alginate	94:108	Sodium alginate (NaA)	94:114	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	3	20	theme	physiological	431:443	arg1	environment					445:455	the physiological environment	427:455	the physiological environment	427:455	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	7	21	theme	cell	1189:1192	arg1	adhesion					1194:1201	cell adhesion	1189:1201	cell adhesion	1189:1201	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	1	22	theme	Sodium	94:99	arg1	alginate					101:108	Sodium alginate	94:108	Sodium alginate (NaA)	94:114	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	22	theme	Sodium	94:99	arg1	material					166:173	microfiber-based scaffold material	140:173	microfiber-based scaffold material	140:173	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	22	theme	Sodium	94:99	arg1	NaA					111:113	NaA	111:113	NaA	111:113	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	3	23	theme	microfiber	413:422	arg1	stability					388:396	the stability	384:396	the stability of Ca-alginate microfiber	384:422	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	0	24	theme	Simple	0:5	arg1	fabrication					7:17	Simple fabrication	0:17	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.	0:92	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	7	25	from	adhesion	1194:1201	arg1	microfiber					1222:1231	the microfiber	1218:1231	the microfiber	1218:1231	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	0	26	theme	inner	22:26	arg1	microfiber					60:69	inner chitosan-coated alginate hollow microfiber	22:69	inner chitosan-coated alginate hollow microfiber	22:69	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	9	27	theme	tissue	1450:1455	arg1	engineering					1457:1467	tissue engineering	1450:1467	tissue engineering	1450:1467	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	9	28	dep	culture	1438:1444	arg1	application					1469:1479	application	1469:1479	application	1469:1479	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	11	29	theme	Part	1532:1535	arg1	B					1537:1537	Part B	1532:1537	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.	1513:1574	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.
30784171	6	30	theme	PBS	1025:1027	arg1	solution					1029:1036	PBS solution	1025:1036	PBS solution	1025:1036	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	7	31	theme	affinity	1131:1138	arg1	environment					1108:1118	the inner environment	1098:1118	the inner environment of the low affinity of alginate to cell surfaces	1098:1167	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	5	32	theme	PBS	705:707	arg1	pH					719:720	pH 7.4	719:724	pH 7.4	719:724	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	5	32	theme	PBS	705:707	arg1	solution					709:716	the PBS solution	701:716	the PBS solution (pH 7.4)	701:725	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	1	33	theme	scaffold	157:164	arg1	alginate					101:108	Sodium alginate	94:108	Sodium alginate (NaA)	94:114	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	1	33	theme	scaffold	157:164	arg1	material					166:173	microfiber-based scaffold material	140:173	microfiber-based scaffold material	140:173	Sodium alginate (NaA) has been widely used as microfiber-based scaffold material.
30784171	9	34	theme	methodologies	1396:1408	arg1	development					1370:1380	the development	1366:1380	the development of innovative methodologies and materials for both cell culture and tissue engineering application	1366:1479	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	3	35	theme	inner	461:465	arg1	coating					476:482	an inner chitosan coating	458:482	an inner chitosan coating	458:482	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	0	36	theme	alginate	44:51	arg1	microfiber					60:69	inner chitosan-coated alginate hollow microfiber	22:69	inner chitosan-coated alginate hollow microfiber	22:69	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	6	37	theme	microfiber	923:932	arg1	wall					911:914	the inner wall	901:914	the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution	901:1036	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	7	38	theme	alginate	1143:1150	arg1	affinity					1131:1138	the low affinity	1123:1138	the low affinity of alginate to cell surfaces	1123:1167	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	7	39	theme	inner	1102:1106	arg1	environment					1108:1118	the inner environment	1098:1118	the inner environment of the low affinity of alginate to cell surfaces	1098:1167	In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber.
30784171	0	40	theme	chitosan-coated	28:42	arg1	microfiber					60:69	inner chitosan-coated alginate hollow microfiber	22:69	inner chitosan-coated alginate hollow microfiber	22:69	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	3	41	theme	Ca-alginate	401:411	arg1	microfiber					413:422	Ca-alginate microfiber	401:422	Ca-alginate microfiber	401:422	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	6	42	theme	NaA	838:840	arg1	layer					842:846	an NaA layer	835:846	an NaA layer	835:846	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	2	43	theme	physiological	234:246	arg1	environment					248:258	the physiological environment	230:258	the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering	230:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	8	44	theme	HepG2	1234:1238	arg1	cells					1240:1244	HepG2 cells	1234:1244	HepG2 cells in the microfibers	1234:1263	HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability.
30784171	0	45	theme	microfiber	60:69	arg1	fabrication					7:17	Simple fabrication	0:17	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.	0:92	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	5	46	theme	microfiber	675:684	arg1	stability					658:666	The stability	654:666	The stability of the microfiber	654:684	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	10	47	dep	©	1482:1482	arg1	Inc.					1508:1511	Inc.	1508:1511	Inc.	1508:1511	© 2019 Wiley Periodicals, Inc.
30784171	2	48	theme	tissue	339:344	arg1	engineering					346:356	tissue engineering	339:356	tissue engineering	339:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	0	49	theme	hollow	53:58	arg1	microfiber					60:69	inner chitosan-coated alginate hollow microfiber	22:69	inner chitosan-coated alginate hollow microfiber	22:69	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	4	50	with	microfiber	595:604	arg1	layers					632:637	double cross-linking layers	611:637	double cross-linking layers	611:637	A more stable composite microfiber with double cross-linking layers was generated.
30784171	0	51	with	fabrication	7:17	arg1	stability					83:91	higher stability	76:91	higher stability	76:91	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	6	52	theme	inner	905:909	arg1	wall					911:914	the inner wall	901:914	the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution	901:1036	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	5	53	from	effect	751:756	arg1	structure					772:780	the hollow structure	761:780	the hollow structure	761:780	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	8	54	dep	viability	1290:1298	arg1	ability					1318:1324	ability	1318:1324	ability	1318:1324	HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability.
30784171	3	55	theme	chitosan	467:474	arg1	coating					476:482	an inner chitosan coating	458:482	an inner chitosan coating	458:482	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	6	56	theme	hollow	956:961	arg1	region					963:968	the hollow region	952:968	the hollow region	952:968	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	6	57	theme	chitosan	809:816	arg1	component					818:826	chitosan component	809:826	chitosan component	809:826	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	8	58	theme	cell	1285:1288	arg1	viability					1290:1298	favorable cell viability	1275:1298	favorable cell viability	1275:1298	HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability.
30784171	8	59	from	cells	1240:1244	arg1	microfibers					1253:1263	the microfibers	1249:1263	the microfibers	1249:1263	HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability.
30784171	2	60	theme	long-term	314:322	arg1	application					324:334	its long-term application	310:334	its long-term application in tissue engineering	310:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	6	61	theme	Ca-alginate	986:996	arg1	shell					998:1002	the Ca-alginate shell	982:1002	the Ca-alginate shell	982:1002	The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution.
30784171	0	62	theme	higher	76:81	arg1	stability					83:91	higher stability	76:91	higher stability	76:91	Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.
30784171	2	63	theme	Ca-alginate	185:195	arg1	microfiber					197:206	Ca-alginate microfiber	185:206	Ca-alginate microfiber	185:206	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	9	64	theme	cell	1433:1436	arg1	culture					1438:1444	cell culture	1433:1444	cell culture	1433:1444	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	3	65	theme	hollow	514:519	arg1	microfiber					521:530	Ca-alginate hollow microfiber	502:530	Ca-alginate hollow microfiber	502:530	In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device.
30784171	9	66	theme	materials	1414:1422	arg1	development					1370:1380	the development	1366:1380	the development of innovative methodologies and materials for both cell culture and tissue engineering application	1366:1479	We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application.
30784171	4	67	theme	cross-linking	618:630	arg1	layers					632:637	double cross-linking layers	611:637	double cross-linking layers	611:637	A more stable composite microfiber with double cross-linking layers was generated.
30784171	5	68	theme	hollow	765:770	arg1	structure					772:780	the hollow structure	761:780	the hollow structure	761:780	The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure.
30784171	2	69	theme	due	260:262	arg1	environment					248:258	the physiological environment	230:258	the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering	230:356	However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering.
30784171	8	70	theme	favorable	1275:1283	arg1	viability					1290:1298	favorable cell viability	1275:1298	favorable cell viability	1275:1298	HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability.
30784171	11	71	dep	Res	1528:1530	arg1	B					1537:1537	Part B	1532:1537	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.	1513:1574	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.
30784171	11	71	dep	Res	1528:1530	arg1	Biomater					1545:1552	Biomater	1545:1552	Biomater	1545:1552	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.
30784171	11	71	dep	Res	1528:1530	arg1	2019					1570:1573	2019	1570:1573	2019	1570:1573	J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.
30784171	4	72	theme	double	611:616	arg1	layers					632:637	double cross-linking layers	611:637	double cross-linking layers	611:637	A more stable composite microfiber with double cross-linking layers was generated.
31103818	6	0	theme	pellet	1033:1038	arg1	size					1040:1043	pellet size	1033:1043	pellet size	1033:1043	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	3	1	theme	typical	676:682	arg1	deviations					692:701	ii) typical process deviations	672:701	ii) typical process deviations	672:701	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	6	2	theme	increased	1114:1122	arg1	throughput					1124:1133	an increased throughput	1111:1133	an increased throughput	1111:1133	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	3	3	theme	feeding	710:716	arg1	errors					718:723	feeding errors	710:723	feeding errors	710:723	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	3	4	theme	quality	564:570	arg1	sensitivity					541:551	the sensitivity	537:551	the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors,	537:724	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	10	5	theme	HPMC	1822:1825	arg1	content					1827:1833	high HPMC content	1817:1833	high HPMC content	1817:1833	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	6	6	theme	insufficient	1157:1168	arg1	cooling					1177:1183	the resulting insufficient strand cooling	1143:1183	the resulting insufficient strand cooling before the pelletizer	1143:1205	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	10	7	theme	processing	1796:1805	arg1	melt					1807:1810	processing melt	1796:1810	processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions	1796:1900	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	1	8	theme	hot-melt	172:179	arg1	extrusion					181:189	a robust hot-melt extrusion and strand pelletization process	163:222	extrusion	181:189	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	4	9	theme	limiting	871:878	arg1	factor					880:885	a limiting factor	869:885	a limiting factor for process stability	869:907	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	10	10	theme	NIR-based	1695:1703	arg1	monitoring					1717:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	7	11	theme	settings	1324:1331	arg1	range					1291:1295	the entire investigated range	1267:1295	the entire investigated range of formulation and process settings	1267:1331	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	3	12	theme	process	573:579	arg1	stability					581:589	process stability	573:589	process stability	573:589	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	9	13	theme	residence	1611:1619	arg1	RTD					1640:1642	RTD	1640:1642	RTD	1640:1642	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	9	13	theme	residence	1611:1619	arg1	distribution					1626:1637	the residence time distribution	1607:1637	the residence time distribution (RTD)	1607:1643	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	6	14	theme	strand	1170:1175	arg1	cooling					1177:1183	the resulting insufficient strand cooling	1143:1183	the resulting insufficient strand cooling before the pelletizer	1143:1205	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	10	15	theme	content	1709:1715	arg1	monitoring					1717:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	1	16	theme	pelletization	202:214	arg1	process					216:222	a robust hot-melt extrusion and strand pelletization process	163:222	process	216:222	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	9	17	theme	distribution	1626:1637	arg1	sensitivity					1592:1602	the sensitivity	1588:1602	the sensitivity of the residence time distribution (RTD)	1588:1643	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	6	18	theme	resulting	1147:1155	arg1	cooling					1177:1183	the resulting insufficient strand cooling	1143:1183	the resulting insufficient strand cooling before the pelletizer	1143:1205	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	10	19	theme	purging	1853:1859	arg1	lack					1845:1848	a lack	1843:1848	a lack of purging by less viscous formulation compositions	1843:1900	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	3	20	theme	process	596:602	arg1	tools					615:619	process monitoring tools	596:619	process monitoring tools	596:619	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	8	21	theme	pellets	1382:1388	arg1	production					1365:1374	the production	1361:1374	the production of IR pellets for subsequent tableting	1361:1413	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	7	22	theme	investigated	1278:1289	arg1	range					1291:1295	the entire investigated range	1267:1295	the entire investigated range of formulation and process settings	1267:1331	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	3	23	theme	parameter	627:635	arg1	changes					637:643	i) parameter changes	624:643	i) parameter changes	624:643	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	8	24	theme	subsequent	1394:1403	arg1	tableting					1405:1413	subsequent tableting	1394:1413	subsequent tableting	1394:1413	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	10	25	theme	formulation	1877:1887	arg1	compositions					1889:1900	less viscous formulation compositions	1864:1900	less viscous formulation compositions	1864:1900	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	0	26	theme	pelletization	58:70	arg1	line					72:75	a continuous hot-melt extrusion and strand pelletization line	15:75	line	72:75	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	1	27	theme	pharmaceutical	314:327	arg1	API					341:343	API	341:343	API	341:343	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	27	theme	pharmaceutical	314:327	arg1	nimodipine					347:356	nimodipine	347:356	nimodipine	347:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	27	theme	pharmaceutical	314:327	arg1	ingredient					329:338	a poorly water-soluble active pharmaceutical ingredient	284:338	a poorly water-soluble active pharmaceutical ingredient (API)	284:344	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	28	theme	work	143:146	arg1	purpose					127:133	The purpose	123:133	The purpose of this work	123:146	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	2	29	theme	traditional	487:497	arg1	batch-manufacturing					499:517	traditional batch-manufacturing	487:517	traditional batch-manufacturing	487:517	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	5	30	from	filler	995:1000	arg1	melt					1009:1012	the melt	1005:1012	the melt	1005:1012	Especially critical were deviations to increased HPMC content, since it behaved as a filler in the melt.
31103818	4	31	dep	factor	880:885	arg1	i.e.					864:867	i.e.	864:867	i.e.	864:867	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	2	32	theme	pharmaceutical	377:390	arg1	processes					417:425	pharmaceutical continuous manufacturing processes	377:425	pharmaceutical continuous manufacturing processes	377:425	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	0	33	theme	composition	100:110	arg1	variation					112:120	composition variation	100:120	composition variation	100:120	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	2	34	theme	strategy	446:453	arg1	vital					458:462	vital	458:462	vital	458:462	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	2	34	theme	strategy	446:453	arg1	robustness					363:372	The robustness	359:372	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy	359:453	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	10	35	with	melt	1807:1810	arg1	content					1827:1833	high HPMC content	1817:1833	high HPMC content	1817:1833	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	8	36	theme	control	1457:1463	arg1	strategy					1465:1472	a technical control strategy	1445:1472	a technical control strategy	1445:1472	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	4	37	dep	pressure	795:802	arg1	die					786:788	die	786:788	die	786:788	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	3	38	dep	deviations	692:701	arg1	ii					672:673	ii	672:673	ii	672:673	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	3	39	theme	control	652:658	arg1	actions					660:666	control actions	652:666	control actions	652:666	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	2	40	theme	manufacturing	403:415	arg1	processes					417:425	pharmaceutical continuous manufacturing processes	377:425	pharmaceutical continuous manufacturing processes	377:425	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	0	41	theme	hot-melt	28:35	arg1	extrusion					37:45	a continuous hot-melt extrusion and strand pelletization line	15:75	extrusion	37:45	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	10	42	theme	material	1747:1754	arg1	tracking					1756:1763	material tracking	1747:1763	material tracking	1747:1763	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	4	43	theme	melt	790:793	arg1	pressure					795:802	die melt pressure	786:802	die melt pressure	786:802	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	1	44	theme	water-soluble	293:305	arg1	API					341:343	API	341:343	API	341:343	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	44	theme	water-soluble	293:305	arg1	nimodipine					347:356	nimodipine	347:356	nimodipine	347:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	44	theme	water-soluble	293:305	arg1	ingredient					329:338	a poorly water-soluble active pharmaceutical ingredient	284:338	a poorly water-soluble active pharmaceutical ingredient (API)	284:344	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	8	45	theme	design	1344:1349	arg1	space					1351:1355	a design space	1342:1355	a design space for the production of IR pellets for subsequent tableting	1342:1413	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	5	46	theme	HPMC	959:962	arg1	content					964:970	increased HPMC content	949:970	increased HPMC content	949:970	Especially critical were deviations to increased HPMC content, since it behaved as a filler in the melt.
31103818	7	47	theme	API	1208:1210	arg1	dissolution					1212:1222	API dissolution	1208:1222	API dissolution	1208:1222	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	10	48	theme	viscous	1869:1875	arg1	compositions					1889:1900	less viscous formulation compositions	1864:1900	less viscous formulation compositions	1864:1900	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	3	49	theme	process	684:690	arg1	deviations					692:701	ii) typical process deviations	672:701	ii) typical process deviations	672:701	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	3	50	theme	product	556:562	arg1	quality					564:570	product quality	556:570	product quality	556:570	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	1	51	theme	immediate	258:266	arg1	IR					277:278	IR	277:278	IR	277:278	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	51	theme	immediate	258:266	arg1	release					268:274	an immediate release	255:274	an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine	255:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	9	52	theme	API	1572:1574	arg1	content					1576:1582	API content	1572:1582	API content	1572:1582	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	10	53	theme	high	1817:1820	arg1	content					1827:1833	high HPMC content	1817:1833	high HPMC content	1817:1833	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	3	54	theme	stability	581:589	arg1	sensitivity					541:551	the sensitivity	537:551	the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors,	537:724	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	1	55	theme	robust	165:170	arg1	extrusion					181:189	a robust hot-melt extrusion and strand pelletization process	163:222	extrusion	181:189	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	7	56	from	dissolution	1212:1222	arg1	contrast					1227:1234	contrast	1227:1234	contrast	1227:1234	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	10	57	theme	due	1836:1838	arg1	content					1827:1833	high HPMC content	1817:1833	high HPMC content	1817:1833	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	3	58	theme	monitoring	604:613	arg1	tools					615:619	process monitoring tools	596:619	process monitoring tools	596:619	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	1	59	theme	strand	195:200	arg1	process					216:222	a robust hot-melt extrusion and strand pelletization process	163:222	process	216:222	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	9	60	theme	time	1621:1624	arg1	RTD					1640:1642	RTD	1640:1642	RTD	1640:1642	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	9	60	theme	time	1621:1624	arg1	distribution					1626:1637	the residence time distribution	1607:1637	the residence time distribution (RTD)	1607:1643	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	8	61	theme	IR	1379:1380	arg1	pellets					1382:1388	IR pellets	1379:1388	IR pellets for subsequent tableting	1379:1413	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	7	62	theme	process	1316:1322	arg1	settings					1324:1331	formulation and process settings	1300:1331	formulation and process settings	1300:1331	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	1	63	theme	active	307:312	arg1	API					341:343	API	341:343	API	341:343	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	63	theme	active	307:312	arg1	nimodipine					347:356	nimodipine	347:356	nimodipine	347:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	63	theme	active	307:312	arg1	ingredient					329:338	a poorly water-soluble active pharmaceutical ingredient	284:338	a poorly water-soluble active pharmaceutical ingredient (API)	284:344	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	3	64	theme	experiments	758:768	arg1	design					748:753	a design	746:753	a design of experiments (DoE)	746:774	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	10	65	theme	API	1705:1707	arg1	monitoring					1717:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring	1695:1726	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	4	66	theme	composition	840:850	arg1	deviations					852:861	composition deviations	840:861	composition deviations	840:861	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	7	67	theme	formulation	1300:1310	arg1	settings					1324:1331	formulation and process settings	1300:1331	formulation and process settings	1300:1331	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	6	68	theme	due	1136:1138	arg1	throughput					1124:1133	an increased throughput	1111:1133	an increased throughput	1111:1133	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	0	69	theme	line	72:75	arg1	Sensitivity					0:10	Sensitivity	0:10	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.	0:121	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	10	70	theme	RTD	1732:1734	arg1	models					1736:1741	RTD models	1732:1741	RTD models for material tracking	1732:1763	NIR-based API content monitoring and RTD models for material tracking were found to be at risk after processing melt with high HPMC content, due to a lack of purging by less viscous formulation compositions.
31103818	7	71	theme	entire	1271:1276	arg1	range					1291:1295	the entire investigated range	1267:1295	the entire investigated range of formulation and process settings	1267:1331	API dissolution in contrast, was found to be robust across the entire investigated range of formulation and process settings.
31103818	9	72	theme	tracer	1674:1679	arg1	measurements					1681:1692	tracer measurements	1674:1692	tracer measurements	1674:1692	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	0	73	theme	control	80:86	arg1	actions					88:94	control actions	80:94	control actions	80:94	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	1	74	theme	ingredient	329:338	arg1	IR					277:278	IR	277:278	IR	277:278	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	74	theme	ingredient	329:338	arg1	release					268:274	an immediate release	255:274	an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine	255:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	4	75	theme	process	891:897	arg1	stability					899:907	process stability	891:907	process stability	891:907	Thereby, die melt pressure was found to be highly sensitive to composition deviations, i.e. a limiting factor for process stability.
31103818	6	76	theme	size	1049:1052	arg1	distribution					1054:1065	size distribution	1049:1065	size distribution	1049:1065	Pelletization, or pellet size and size distribution respectively, were found to be sensitive to an increased throughput, due to the resulting insufficient strand cooling before the pelletizer.
31103818	8	77	theme	technical	1447:1455	arg1	strategy					1465:1472	a technical control strategy	1445:1472	a technical control strategy	1445:1472	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	2	78	theme	control	438:444	arg1	strategy					446:453	its control strategy	434:453	its control strategy	434:453	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	0	79	theme	continuous	17:26	arg1	extrusion					37:45	a continuous hot-melt extrusion and strand pelletization line	15:75	extrusion	37:45	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	2	80	theme	processes	417:425	arg1	vital					458:462	vital	458:462	vital	458:462	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	2	80	theme	processes	417:425	arg1	robustness					363:372	The robustness	359:372	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy	359:453	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	0	81	theme	extrusion	37:45	arg1	Sensitivity					0:10	Sensitivity	0:10	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.	0:121	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	2	82	theme	continuous	392:401	arg1	processes					417:425	pharmaceutical continuous manufacturing processes	377:425	pharmaceutical continuous manufacturing processes	377:425	The robustness of pharmaceutical continuous manufacturing processes and of its control strategy is vital for competitiveness to traditional batch-manufacturing.
31103818	0	83	theme	strand	51:56	arg1	line					72:75	a continuous hot-melt extrusion and strand pelletization line	15:75	line	72:75	Sensitivity of a continuous hot-melt extrusion and strand pelletization line to control actions and composition variation.
31103818	9	84	theme	Near-infrared	1516:1528	arg1	spectroscopy					1536:1547	Near-infrared (NIR) spectroscopy	1516:1547	Near-infrared (NIR) spectroscopy	1516:1547	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	9	84	theme	Near-infrared	1516:1528	arg1	NIR					1531:1533	NIR	1531:1533	NIR	1531:1533	Near-infrared (NIR) spectroscopy was applied to monitor API content and the sensitivity of the residence time distribution (RTD) was investigated by means of tracer measurements.
31103818	8	85	theme	robust	1500:1505	arg1	process					1507:1513	a robust process	1498:1513	a robust process	1498:1513	Second, a design space for the production of IR pellets for subsequent tableting was established, and finally, a technical control strategy was developed to ensure a robust process.
31103818	3	86	dep	errors	718:723	arg1	i.e.					704:707	i.e.	704:707	i.e.	704:707	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	5	87	theme	increased	949:957	arg1	content					964:970	increased HPMC content	949:970	increased HPMC content	949:970	Especially critical were deviations to increased HPMC content, since it behaved as a filler in the melt.
31103818	3	88	theme	tools	615:619	arg1	sensitivity					541:551	the sensitivity	537:551	the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors,	537:724	Therefore, first the sensitivity of product quality, process stability, and process monitoring tools to i) parameter changes due to control actions and ii) typical process deviations, i.e., feeding errors, was investigated in a design of experiments (DoE).
31103818	1	89	with	pellets	242:248	arg1	IR					277:278	IR	277:278	IR	277:278	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31103818	1	89	with	pellets	242:248	arg1	release					268:274	an immediate release	255:274	an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine	255:356	The purpose of this work was to develop a robust hot-melt extrusion and strand pelletization process for manufacturing pellets with an immediate release (IR) of a poorly water-soluble active pharmaceutical ingredient (API), nimodipine.
31590885	0	0	theme	capacity	78:85	arg1	regulation					46:55	the regulation	42:55	the regulation of the water-binding capacity of potato cell walls	42:106	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	4	1	theme	in	430:431	arg1	exo-truncation					438:451	in vivo exo-truncation	430:451	in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains	430:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	5	2	theme	side-chains	556:566	arg1	exo-truncation					532:545	exo-truncation	532:545	exo-truncation of these side-chains	532:566	In contrast, exo-truncation of these side-chains increased the WBC by 13% in vitro.
31590885	0	3	theme	water-binding	64:76	arg1	capacity					78:85	the water-binding capacity	60:85	the water-binding capacity of potato cell walls	60:106	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	8	4	from	added-value	1090:1100	arg1	food					1105:1108	food	1105:1108	food	1105:1108	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	8	4	from	added-value	1090:1100	arg1	products					1123:1130	biobased products	1114:1130	biobased products	1114:1130	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	6	5	theme	wall	696:699	arg1	remodelling					701:711	cell wall remodelling	691:711	cell wall remodelling	691:711	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	0	6	theme	potato	90:95	arg1	walls					102:106	potato cell walls	90:106	potato cell walls	90:106	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	6	7	theme	cell	691:694	arg1	remodelling					701:711	cell wall remodelling	691:711	cell wall remodelling	691:711	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	6	8	theme	RG-I	634:637	arg1	side-chains					648:658	RG-I galactan side-chains	634:658	RG-I galactan side-chains	634:658	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	2	9	theme	water-binding	237:249	arg1	WBC					261:263	WBC	261:263	WBC	261:263	Valorisation of PCW is hindered by its high water-binding capacity (WBC).
31590885	2	9	theme	water-binding	237:249	arg1	capacity					251:258	its high water-binding capacity	228:258	its high water-binding capacity (WBC)	228:264	Valorisation of PCW is hindered by its high water-binding capacity (WBC).
31590885	0	10	theme	walls	102:106	arg1	capacity					78:85	the water-binding capacity	60:85	the water-binding capacity of potato cell walls	60:106	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	2	11	theme	high	232:235	arg1	WBC					261:263	WBC	261:263	WBC	261:263	Valorisation of PCW is hindered by its high water-binding capacity (WBC).
31590885	2	11	theme	high	232:235	arg1	capacity					251:258	its high water-binding capacity	228:258	its high water-binding capacity (WBC)	228:264	Valorisation of PCW is hindered by its high water-binding capacity (WBC).
31590885	0	12	theme	cell	97:100	arg1	walls					102:106	potato cell walls	90:106	potato cell walls	90:106	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	3	13	theme	important	348:356	arg1	roles					358:362	important roles	348:362	important roles	348:362	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	1	14	theme	low	139:141	arg1	by-product					149:158	a low value by-product	137:158	a low value by-product from the potato starch industry	137:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	14	theme	low	139:141	arg1	walls					121:125	Potato cell walls	109:125	Potato cell walls (PCW)	109:131	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	0	15	theme	galactan	5:12	arg1	side-chains					14:24	RG-I galactan side-chains	0:24	RG-I galactan side-chains	0:24	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	8	16	theme	wall	1047:1050	arg1	by-products					1052:1062	cell wall by-products	1042:1062	cell wall by-products	1042:1062	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	6	17	theme	PCW	679:681	arg1	WBC					672:674	the WBC	668:674	the WBC of PCW	668:681	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	1	18	theme	value	143:147	arg1	by-product					149:158	a low value by-product	137:158	a low value by-product from the potato starch industry	137:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	18	theme	value	143:147	arg1	walls					121:125	Potato cell walls	109:125	Potato cell walls (PCW)	109:131	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	0	19	theme	RG-I	0:3	arg1	side-chains					14:24	RG-I galactan side-chains	0:24	RG-I galactan side-chains	0:24	RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls.
31590885	4	20	theme	-D-galactan	468:478	arg1	side-chains					480:490	RG-I β-(1→4)-D-galactan side-chains	456:490	RG-I β-(1→4)-D-galactan side-chains	456:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	8	21	theme	Better	943:948	arg1	understanding					950:962	Better understanding	943:962	Better understanding of structure-function relationships of pectin macromolecules	943:1023	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	8	22	theme	structure-function	967:984	arg1	relationships					986:998	structure-function relationships	967:998	structure-function relationships of pectin macromolecules	967:1023	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	7	23	from	architecture	888:899	arg1	matrix					935:940	the matrix	931:940	the matrix	931:940	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	8	24	theme	biobased	1114:1121	arg1	products					1123:1130	biobased products	1114:1130	biobased products	1114:1130	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	4	25	theme	side-chains	480:490	arg1	exo-truncation					438:451	in vivo exo-truncation	430:451	in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains	430:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	3	26	theme	wall	398:401	arg1	matrix					403:408	the cell wall matrix	389:408	the cell wall matrix	389:408	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	1	27	theme	potato	169:174	arg1	industry					183:190	the potato starch industry	165:190	the potato starch industry	165:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	4	28	theme	β-	461:462	arg1	side-chains					480:490	RG-I β-(1→4)-D-galactan side-chains	456:490	RG-I β-(1→4)-D-galactan side-chains	456:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	8	29	theme	relationships	986:998	arg1	understanding					950:962	Better understanding	943:962	Better understanding of structure-function relationships of pectin macromolecules	943:1023	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	1	30	theme	starch	176:181	arg1	industry					183:190	the potato starch industry	165:190	the potato starch industry	165:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	4	31	theme	RG-I	456:459	arg1	side-chains					480:490	RG-I β-(1→4)-D-galactan side-chains	456:490	RG-I β-(1→4)-D-galactan side-chains	456:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	8	32	theme	macromolecules	1010:1023	arg1	relationships					986:998	structure-function relationships	967:998	structure-function relationships of pectin macromolecules	967:1023	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	4	33	theme	1→4	464:466	arg1	side-chains					480:490	RG-I β-(1→4)-D-galactan side-chains	456:490	RG-I β-(1→4)-D-galactan side-chains	456:490	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	8	34	theme	pectin	1003:1008	arg1	macromolecules					1010:1023	pectin macromolecules	1003:1023	pectin macromolecules	1003:1023	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	6	35	theme	side-chains	648:658	arg1	degradation					619:629	degradation	619:629	degradation of RG-I galactan side-chains	619:658	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	1	36	theme	Potato	109:114	arg1	PCW					128:130	PCW	128:130	PCW	128:130	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	36	theme	Potato	109:114	arg1	walls					121:125	Potato cell walls	109:125	Potato cell walls (PCW)	109:131	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	36	theme	Potato	109:114	arg1	by-product					149:158	a low value by-product	137:158	a low value by-product from the potato starch industry	137:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	7	37	theme	RG-I	792:795	arg1	side-chains					806:816	RG-I galactan side-chains	792:816	RG-I galactan side-chains	792:816	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	4	38	dep	in	430:431	arg1	vivo					433:436	vivo	433:436	vivo	433:436	Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6-9%.
31590885	1	39	from	industry	183:190	arg1	by-product					149:158	a low value by-product	137:158	a low value by-product from the potato starch industry	137:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	39	from	industry	183:190	arg1	walls					121:125	Potato cell walls	109:125	Potato cell walls (PCW)	109:131	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	6	40	theme	galactan	639:646	arg1	side-chains					648:658	RG-I galactan side-chains	634:658	RG-I galactan side-chains	634:658	We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity.
31590885	1	41	theme	cell	116:119	arg1	PCW					128:130	PCW	128:130	PCW	128:130	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	41	theme	cell	116:119	arg1	walls					121:125	Potato cell walls	109:125	Potato cell walls (PCW)	109:131	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	1	41	theme	cell	116:119	arg1	by-product					149:158	a low value by-product	137:158	a low value by-product from the potato starch industry	137:190	Potato cell walls (PCW) are a low value by-product from the potato starch industry.
31590885	7	42	theme	galactan	797:804	arg1	side-chains					806:816	RG-I galactan side-chains	792:816	RG-I galactan side-chains	792:816	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	3	43	from	WBC	382:384	arg1	matrix					403:408	the cell wall matrix	389:408	the cell wall matrix	389:408	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	7	44	from	interactions	915:926	arg1	matrix					935:940	the matrix	931:940	the matrix	931:940	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	8	45	theme	cell	1042:1045	arg1	by-products					1052:1062	cell wall by-products	1042:1062	cell wall by-products	1042:1062	Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.
31590885	7	46	theme	polysaccharide	873:886	arg1	spacing					902:908	spacing	902:908	spacing	902:908	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	7	46	theme	polysaccharide	873:886	arg1	architecture					888:899	polysaccharide architecture	873:899	polysaccharide architecture (spacing)	873:909	Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix.
31590885	3	47	theme	cell	393:396	arg1	matrix					403:408	the cell wall matrix	389:408	the cell wall matrix	389:408	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	3	48	theme	polysaccharides	286:300	arg1	interactions					306:317	interactions	306:317	interactions between these entities	306:340	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	3	48	theme	polysaccharides	286:300	arg1	composition					271:281	The composition	267:281	The composition of polysaccharides	267:300	The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix.
31590885	2	49	theme	PCW	209:211	arg1	Valorisation					193:204	Valorisation	193:204	Valorisation of PCW	193:211	Valorisation of PCW is hindered by its high water-binding capacity (WBC).
31271400	2	0	theme	necrosis	487:494	arg1	factor					496:501	tumor necrosis factor (TNF)-α	481:509	tumor necrosis factor (TNF)-α	481:509	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	0	theme	necrosis	487:494	arg1	TNF					504:506	TNF	504:506	TNF	504:506	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	3	1	theme	immunoglobulin	673:686	arg1	SIgA					691:694	SIgA	691:694	SIgA	691:694	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	3	1	theme	immunoglobulin	673:686	arg1	A					688:688	secretory immunoglobulin A	663:688	secretory immunoglobulin A (SIgA)	663:695	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	1	2	theme	immunostimulatory	168:184	arg1	activity					186:193	the immunostimulatory activity	164:193	the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP)	164:240	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	2	3	theme	tumor	481:485	arg1	factor					496:501	tumor necrosis factor (TNF)-α	481:509	tumor necrosis factor (TNF)-α	481:509	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	3	theme	tumor	481:485	arg1	TNF					504:506	TNF	504:506	TNF	504:506	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	4	theme	splenic	565:571	arg1	tissues					585:591	thymic, splenic and colonic tissues	557:591	thymic, splenic and colonic tissues of the immunosuppressed mice	557:620	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	5	5	theme	SCFA	1194:1197	arg1	production					1199:1208	SCFA production	1194:1208	SCFA production	1194:1208	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	4	6	theme	diversity	902:910	arg1	production					841:850	the production	837:850	the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	837:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	7	theme	Clostridium_XVIII	1017:1033	arg1	growth					956:961	the growth	952:961	the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	952:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	1	8	theme	-induced	273:280	arg1	mice					306:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	4	9	theme	microbiota	891:900	arg1	diversity					902:910	microbiota diversity	891:910	microbiota diversity	891:910	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	2	10	theme	thymic	557:562	arg1	tissues					585:591	thymic, splenic and colonic tissues	557:591	thymic, splenic and colonic tissues of the immunosuppressed mice	557:620	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	11	theme	mice	617:620	arg1	tissues					585:591	thymic, splenic and colonic tissues	557:591	thymic, splenic and colonic tissues of the immunosuppressed mice	557:620	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	11	theme	mice	617:620	arg1	serum					547:551	serum	547:551	serum	547:551	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	12	theme	body	377:380	arg1	weight					382:387	body weight	377:387	body weight	377:387	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	1	13	theme	immunosuppressed	282:297	arg1	mice					306:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	0	14	from	activity	68:75	arg1	correlation					105:115	correlation	105:115	correlation	105:115	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	0	14	from	activity	68:75	arg1	mice					97:100	immunosuppressed mice	80:100	immunosuppressed mice	80:100	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	2	15	theme	immunosuppressed	600:615	arg1	mice					617:620	the immunosuppressed mice	596:620	the immunosuppressed mice	596:620	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	5	16	theme	systemic	1322:1329	arg1	immunity					1331:1338	the systemic immunity	1318:1338	the systemic immunity	1318:1338	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	2	17	theme	thymus/spleen	407:419	arg1	indexes					421:427	thymus/spleen indexes	407:427	thymus/spleen indexes	407:427	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	0	18	theme	gut	133:135	arg1	microbiota					137:146	gut microbiota	133:146	gut microbiota	133:146	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	3	19	theme	A	688:688	arg1	production					649:658	the production	645:658	the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice	645:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	3	20	from	production	649:658	arg1	tissue					723:728	the colonic tissue	711:728	the colonic tissue of the immunosuppressed mice	711:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	0	21	theme	Caulerpa	0:7	arg1	polysaccharides					22:36	Caulerpa lentillifera polysaccharides	0:36	Caulerpa lentillifera polysaccharides	0:36	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	4	22	theme	Barnesiella	1117:1127	arg1	populations					1089:1099	the microbial populations	1075:1099	the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae	1075:1147	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	1	23	theme	Caulerpa	198:205	arg1	CLP					237:239	CLP	237:239	CLP	237:239	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	1	23	theme	Caulerpa	198:205	arg1	polysaccharides					220:234	Caulerpa lentillifera polysaccharides	198:234	Caulerpa lentillifera polysaccharides (CLP)	198:240	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	0	24	theme	lentillifera	9:20	arg1	polysaccharides					22:36	Caulerpa lentillifera polysaccharides	0:36	Caulerpa lentillifera polysaccharides	0:36	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	6	25	theme	novel	1468:1472	arg1	prebiotics					1474:1483	novel prebiotics	1468:1483	novel prebiotics	1468:1483	The results could be helpful for understanding the functions of CLP, supporting their potential as novel prebiotics and immunostimulators.
31271400	1	26	theme	lentillifera	207:218	arg1	CLP					237:239	CLP	237:239	CLP	237:239	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	1	26	theme	lentillifera	207:218	arg1	polysaccharides					220:234	Caulerpa lentillifera polysaccharides	198:234	Caulerpa lentillifera polysaccharides (CLP)	198:240	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	2	27	theme	interleukin	460:470	arg1	secretions					446:455	the secretions	442:455	the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD)	442:542	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	1	28	theme	BALB/c	299:304	arg1	mice					306:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	cytoxan (CTX)-induced immunosuppressed BALB/c mice	260:309	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	2	29	theme	colonic	577:583	arg1	tissues					585:591	thymic, splenic and colonic tissues	557:591	thymic, splenic and colonic tissues of the immunosuppressed mice	557:620	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	30	theme	colon	390:394	arg1	length					396:401	colon length	390:401	colon length	390:401	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	4	31	theme	Coriobacteriaceae	981:997	arg1	growth					956:961	the growth	952:961	the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	952:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	32	theme	microbial	1079:1087	arg1	populations					1089:1099	the microbial populations	1075:1099	the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae	1075:1147	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	0	33	theme	immunostimulatory	50:66	arg1	activity					68:75	the immunostimulatory activity	46:75	the immunostimulatory activity in immunosuppressed mice in correlation	46:115	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	3	34	theme	mucin2	701:706	arg1	production					649:658	the production	645:658	the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice	645:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	2	35	theme	dismutase	528:536	arg1	secretions					446:455	the secretions	442:455	the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD)	442:542	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	1	36	theme	polysaccharides	220:234	arg1	activity					186:193	the immunostimulatory activity	164:193	the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP)	164:240	In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice.
31271400	2	37	theme	superoxidase	515:526	arg1	SOD					539:541	SOD	539:541	SOD	539:541	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	2	37	theme	superoxidase	515:526	arg1	dismutase					528:536	superoxidase dismutase	515:536	superoxidase dismutase (SOD)	515:542	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	4	38	theme	chain	861:865	arg1	SCFAs					880:884	SCFAs	880:884	SCFAs	880:884	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	38	theme	chain	861:865	arg1	acids					873:877	short chain fatty acids	855:877	short chain fatty acids (SCFAs)	855:885	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	38	theme	chain	861:865	arg1	improvement					937:947	improvement	937:947	improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	937:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	0	39	theme	immunosuppressed	80:95	arg1	mice					97:100	immunosuppressed mice	80:100	immunosuppressed mice	80:100	Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota.
31271400	4	40	theme	immunostimulatory	786:802	arg1	effects					804:810	the above immunostimulatory effects	776:810	the above immunostimulatory effects	776:810	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	3	41	theme	colonic	715:721	arg1	tissue					723:728	the colonic tissue	711:728	the colonic tissue of the immunosuppressed mice	711:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	4	42	theme	short	855:859	arg1	SCFAs					880:884	SCFAs	880:884	SCFAs	880:884	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	42	theme	short	855:859	arg1	acids					873:877	short chain fatty acids	855:877	short chain fatty acids (SCFAs)	855:885	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	42	theme	short	855:859	arg1	improvement					937:947	improvement	937:947	improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	937:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	43	theme	above	780:784	arg1	effects					804:810	the above immunostimulatory effects	776:810	the above immunostimulatory effects	776:810	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	2	44	theme	CTX-evoked	356:365	arg1	damage					367:372	the CTX-evoked damage	352:372	the CTX-evoked damage to body weight, colon length and thymus/spleen indexes	352:427	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	4	45	theme	acids	873:877	arg1	production					841:850	the production	837:850	the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	837:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	46	theme	Helicobacter	1039:1050	arg1	growth					956:961	the growth	952:961	the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	952:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	47	from	improvement	937:947	arg1	growth					956:961	the growth	952:961	the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	952:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	48	theme	fatty	867:871	arg1	SCFAs					880:884	SCFAs	880:884	SCFAs	880:884	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	48	theme	fatty	867:871	arg1	acids					873:877	short chain fatty acids	855:877	short chain fatty acids (SCFAs)	855:885	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	48	theme	fatty	867:871	arg1	improvement					937:947	improvement	937:947	improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter	937:1050	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	4	49	theme	Lachnospiraceae	1133:1147	arg1	populations					1089:1099	the microbial populations	1075:1099	the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae	1075:1147	Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae.
31271400	5	50	theme	mucosal	1279:1285	arg1	immunity					1287:1294	the colonic mucosal immunity	1267:1294	the colonic mucosal immunity	1267:1294	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	6	51	theme	CLP	1433:1435	arg1	functions					1420:1428	the functions	1416:1428	the functions	1416:1428	The results could be helpful for understanding the functions of CLP, supporting their potential as novel prebiotics and immunostimulators.
31271400	5	52	theme	colonic	1271:1277	arg1	immunity					1287:1294	the colonic mucosal immunity	1267:1294	the colonic mucosal immunity	1267:1294	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	3	53	theme	immunosuppressed	737:752	arg1	mice					754:757	the immunosuppressed mice	733:757	the immunosuppressed mice	733:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	2	54	theme	factor	496:501	arg1	secretions					446:455	the secretions	442:455	the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD)	442:542	The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice.
31271400	3	55	theme	secretory	663:671	arg1	SIgA					691:694	SIgA	691:694	SIgA	691:694	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	3	55	theme	secretory	663:671	arg1	A					688:688	secretory immunoglobulin A	663:688	secretory immunoglobulin A (SIgA)	663:695	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31271400	5	56	theme	gut	1214:1216	arg1	microbiota					1218:1227	gut microbiota	1214:1227	gut microbiota	1214:1227	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	5	57	theme	immunosuppressed	1236:1251	arg1	mice					1253:1256	the immunosuppressed mice	1232:1256	the immunosuppressed mice	1232:1256	These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen.
31271400	3	58	theme	mice	754:757	arg1	tissue					723:728	the colonic tissue	711:728	the colonic tissue of the immunosuppressed mice	711:757	Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice.
31091565	9	0	theme	P2	1485:1486	arg1	generation					1488:1497	P2 generation	1485:1497	P2 generation	1485:1497	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	10	1	with	group	1707:1711	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	1	with	group	1707:1711	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	2	theme	highest	1722:1728	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	2	theme	highest	1722:1728	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	7	3	theme	samples	997:1003	arg1	group					988:992	A group	986:992	A group of samples	986:1003	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	7	3	theme	samples	997:1003	arg1	samples					997:1003	samples	997:1003	samples	997:1003	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	16	4	theme	in	2673:2674	arg1	experiments					2681:2691	further in vivo experiments	2665:2691	further in vivo experiments	2665:2691	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	16	5	theme	generation	2722:2731	arg1	foundation					2650:2659	a foundation	2648:2659	a foundation for further in vivo experiments	2648:2691	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	16	5	theme	generation	2722:2731	arg1	development					2701:2711	the development	2697:2711	the development of a new generation of GBR membrane	2697:2747	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	16	6	dep	in	2673:2674	arg1	vivo					2676:2679	vivo	2676:2679	vivo	2676:2679	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	16	7	theme	composite	2587:2595	arg1	membrane					2597:2604	The composite membrane	2583:2604	The composite membrane	2583:2604	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	16	8	theme	GBR	2736:2738	arg1	membrane					2740:2747	GBR membrane	2736:2747	GBR membrane	2736:2747	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	9	9	theme	Oral	1297:1300	arg1	Anhui					1364:1368	Anhui	1364:1368	Anhui	1364:1368	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	9	9	theme	Oral	1297:1300	arg1	Surgery					1320:1326	Oral and Maxillofacial Surgery	1297:1326	Oral and Maxillofacial Surgery	1297:1326	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	9	9	theme	Oral	1297:1300	arg1	Hospital					1342:1349	Hospital	1342:1349	Hospital	1342:1349	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	4	10	theme	universal	718:726	arg1	machine					736:742	mechanical universal testing machine	707:742	mechanical universal testing machine (n=6)	707:748	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	4	10	theme	universal	718:726	arg1	n=6					745:747	n=6	745:747	n=6	745:747	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	10	11	with	Results	1812:1818	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	11	with	Results	1812:1818	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	12	theme	membrane	1873:1880	arg1	cross-section					1842:1854	the cross-section	1838:1854	the cross-section of the composite membrane	1838:1880	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	13	13	theme	membrane	2241:2248	arg1	deacidification					2208:2222	deacidification	2208:2222	deacidification of the composite membrane	2208:2248	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	9	14	theme	Maxillofacial	1306:1318	arg1	Anhui					1364:1368	Anhui	1364:1368	Anhui	1364:1368	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	9	14	theme	Maxillofacial	1306:1318	arg1	Surgery					1320:1326	Oral and Maxillofacial Surgery	1297:1326	Oral and Maxillofacial Surgery	1297:1326	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	9	14	theme	Maxillofacial	1306:1318	arg1	Hospital					1342:1349	Hospital	1342:1349	Hospital	1342:1349	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	14	15	theme	CS	2352:2353	arg1	group					2364:2368	the pure CS membrane group	2343:2368	the pure CS membrane group	2343:2368	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	9	16	theme	staining	1544:1551	arg1	methods					1553:1559	immunocytochemical staining methods	1525:1559	immunocytochemical staining methods	1525:1559	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	10	17	theme	ordered	1892:1898	arg1	structure					1906:1914	an ordered layer structure	1889:1914	an ordered layer structure	1889:1914	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	14	18	from	difference	2310:2319	arg1	absorbance					2324:2333	absorbance	2324:2333	absorbance	2324:2333	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	10	19	theme	control	1637:1643	arg1	group					1645:1649	the blank control group	1627:1649	the blank control group	1627:1649	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	13	20	theme	mass	2072:2075	arg1	%					2105:2105	4.0%	2102:2105	4.0%	2102:2105	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	13	20	theme	mass	2072:2075	arg1	fraction					2077:2084	the mass fraction	2068:2084	the mass fraction of GO in CS	2068:2096	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	15	21	theme	GO	2511:2512	arg1	%					2526:2526	4.0%	2523:2526	4.0%	2523:2526	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	15	21	theme	GO	2511:2512	arg1	fraction					2499:2506	the mass fraction	2490:2506	the mass fraction of GO in CS	2490:2518	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	10	22	theme	CS	1661:1662	arg1	group					1673:1677	the pure CS membrane group	1652:1677	the pure CS membrane group	1652:1677	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	8	23	theme	tensile	1167:1173	arg1	strength					1175:1182	The tensile strength	1163:1182	The tensile strength of the new group of samples	1163:1210	The tensile strength of the new group of samples was tested.
31091565	1	24	theme	gingival	377:384	arg1	HGF					399:401	HGF	399:401	HGF	399:401	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	24	theme	gingival	377:384	arg1	fibroblasts					386:396	human gingival fibroblasts	371:396	human gingival fibroblasts (HGF)	371:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	10	25	theme	pure	1656:1659	arg1	group					1673:1677	the pure CS membrane group	1652:1677	the pure CS membrane group	1652:1677	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	5	26	theme	membrane	787:794	arg1	microstructure					755:768	The microstructure	751:768	The microstructure of the composite membrane with the best tensile strength	751:825	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	15	27	theme	tensile	2565:2571	arg1	strength					2573:2580	the best tensile strength	2556:2580	the best tensile strength	2556:2580	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	0	28	theme	oxide	69:73	arg1	properties					12:21	[Mechanical properties	0:21	[Mechanical properties	0:21	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	0	28	theme	oxide	69:73	arg1	compatibility					32:44	cell compatibility	27:44	cell compatibility	27:44	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	8	29	theme	new	1191:1193	arg1	samples					1204:1210	samples	1204:1210	samples	1204:1210	The tensile strength of the new group of samples was tested.
31091565	8	29	theme	new	1191:1193	arg1	group					1195:1199	the new group	1187:1199	the new group of samples	1187:1210	The tensile strength of the new group of samples was tested.
31091565	0	30	theme	bone	82:85	arg1	membrane					110:117	bone regeneration composite membrane	82:117	bone regeneration composite membrane	82:117	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	1	31	theme	graphene	162:169	arg1	oxide					171:175	graphene oxide	162:175	graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	162:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	2	32	theme	following	460:468	arg1	ratios					470:475	following ratios	460:475	following ratios	460:475	Methods: The CS solution and GO solution were mixed in following ratios, ultrasonically crushed and dispersed, and formed into membranes by self-evaporation.
31091565	0	33	theme	composite	100:108	arg1	membrane					110:117	bone regeneration composite membrane	82:117	bone regeneration composite membrane	82:117	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	1	34	theme	tensile	315:321	arg1	strength					323:330	its tensile strength	311:330	its tensile strength	311:330	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	9	35	theme	gingival	1398:1405	arg1	tissues					1407:1413	the gingival tissues	1394:1413	the gingival tissues remained on the teeth	1394:1435	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	12	36	theme	tensile	2005:2011	arg1	strength					2013:2020	The tensile strength	2001:2020	The tensile strength	2001:2020	The tensile strength increased with the increase of GO ratio.
31091565	7	37	theme	tensile	1059:1065	arg1	membrane					1086:1093	the highest tensile strength composite membrane	1047:1093	the highest tensile strength composite membrane	1047:1093	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	13	38	theme	tensile	2161:2167	arg1	strength					2169:2176	the tensile strength	2157:2176	the tensile strength	2157:2176	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	14	39	theme	control	2448:2454	arg1	P>0.05					2463:2468	P>0.05	2463:2468	P>0.05	2463:2468	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	14	39	theme	control	2448:2454	arg1	group					2456:2460	the blank control group	2438:2460	the blank control group (P>0.05)	2438:2469	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	0	40	theme	[Mechanical	0:10	arg1	properties					12:21	[Mechanical properties	0:21	[Mechanical properties	0:21	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	14	41	theme	CCK-8	2255:2259	arg1	test					2261:2264	The CCK-8 test	2251:2264	The CCK-8 test of HGF	2251:2271	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	11	42	theme	X-ray	1917:1921	arg1	analysis					1935:1942	X-ray diffraction analysis	1917:1942	X-ray diffraction analysis	1917:1942	X-ray diffraction analysis showed that GO could be found in the composite membrane.
31091565	1	43	from	strength	323:330	arg1	proliferation					354:366	the proliferation	350:366	the proliferation of human gingival fibroblasts (HGF)	350:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	44	theme	guided	260:265	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	44	theme	guided	260:265	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	5	45	theme	tensile	810:816	arg1	strength					818:825	the best tensile strength	801:825	the best tensile strength	801:825	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	2	46	theme	CS	418:419	arg1	solution					421:428	The CS solution	414:428	The CS solution	414:428	Methods: The CS solution and GO solution were mixed in following ratios, ultrasonically crushed and dispersed, and formed into membranes by self-evaporation.
31091565	4	47	theme	composite	673:681	arg1	membranes					683:691	the composite membranes	669:691	the composite membranes	669:691	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	1	48	theme	regeneration	272:283	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	48	theme	regeneration	272:283	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	5	49	theme	electron	889:896	arg1	microscopy					898:907	transmission electron microscopy	876:907	transmission electron microscopy	876:907	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	6	50	theme	X-ray	910:914	arg1	diffraction					916:926	X-ray diffraction	910:926	X-ray diffraction	910:926	X-ray diffraction was used to characterize the ingredients of the material.
31091565	12	51	dep	ratio	2056:2060	arg1	GO					2053:2054	GO	2053:2054	GO	2053:2054	The tensile strength increased with the increase of GO ratio.
31091565	10	52	theme	membrane	1664:1671	arg1	group					1673:1677	the pure CS membrane group	1652:1677	the pure CS membrane group	1652:1677	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	1	53	dep	oxide	171:175	arg1	GO					178:179	GO	178:179	GO	178:179	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	10	54	theme	Cell	1562:1565	arg1	counting					1567:1574	Cell counting kit-8	1562:1580	Cell counting kit-8 (CCK-8)	1562:1588	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	54	theme	Cell	1562:1565	arg1	CCK-8					1583:1587	CCK-8	1583:1587	CCK-8	1583:1587	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	1	55	theme	GBR	286:288	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	55	theme	GBR	286:288	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	14	56	theme	pure	2347:2350	arg1	group					2364:2368	the pure CS membrane group	2343:2368	the pure CS membrane group	2343:2368	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	7	57	theme	acid	1149:1152	arg1	removal					1154:1160	acid removal	1149:1160	acid removal	1149:1160	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	10	58	theme	membrane	1698:1705	arg1	group					1707:1711	the composite membrane group	1684:1711	the composite membrane group with the highest tensile strength (n=5)	1684:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	4	59	theme	tensile	649:655	arg1	strength					657:664	The tensile strength	645:664	The tensile strength of the composite membranes	645:691	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	9	60	theme	extracted	1234:1242	arg1	teeth					1253:1257	The newly extracted impacted teeth	1224:1257	The newly extracted impacted teeth	1224:1257	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	5	61	theme	electron	852:859	arg1	microscopy					861:870	scanning electron microscopy	843:870	scanning electron microscopy	843:870	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	14	62	theme	composite	2384:2392	arg1	group					2403:2407	the GO/CS composite membrane group	2374:2407	the GO/CS composite membrane group	2374:2407	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	15	63	from	fraction	2499:2506	arg1	CS					2517:2518	CS	2517:2518	CS	2517:2518	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	1	64	theme	regeneration	208:219	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	64	theme	regeneration	208:219	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	16	65	theme	further	2665:2671	arg1	experiments					2681:2691	further in vivo experiments	2665:2691	further in vivo experiments	2665:2691	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	11	66	located	found	1968:1972	arg2	GO					1956:1957	GO	1956:1957	GO	1956:1957	X-ray diffraction analysis showed that GO could be found in the composite membrane.
31091565	11	66	located	found	1968:1972	arg1	membrane					1991:1998	the composite membrane	1977:1998	the composite membrane	1977:1998	X-ray diffraction analysis showed that GO could be found in the composite membrane.
31091565	7	67	theme	sodium	1119:1124	arg1	solution					1136:1143	a sodium hydroxide solution	1117:1143	a sodium hydroxide solution for acid removal	1117:1160	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	10	68	theme	composite	1863:1871	arg1	membrane					1873:1880	the composite membrane	1859:1880	the composite membrane	1859:1880	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	7	69	theme	membrane	1086:1093	arg1	proportion					1033:1042	the proportion	1029:1042	the proportion of the highest tensile strength composite membrane	1029:1093	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	4	70	theme	testing	728:734	arg1	machine					736:742	mechanical universal testing machine	707:742	mechanical universal testing machine (n=6)	707:748	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	4	70	theme	testing	728:734	arg1	n=6					745:747	n=6	745:747	n=6	745:747	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	13	71	theme	tensile	2112:2118	arg1	strength					2120:2127	the tensile strength	2108:2127	the tensile strength	2108:2127	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	15	72	theme	best	2560:2563	arg1	strength					2573:2580	the best tensile strength	2556:2580	the best tensile strength	2556:2580	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	3	73	from	%	590:590	arg1	CS					628:629	CS	628:629	CS	628:629	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	15	74	contain	has	2552:2554	arg2	strength					2573:2580	the best tensile strength	2556:2580	the best tensile strength	2556:2580	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	15	74	contain	has	2552:2554	arg1	membrane					2543:2550	the composite membrane	2529:2550	the composite membrane	2529:2550	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	16	75	theme	new	2718:2720	arg1	generation					2722:2731	a new generation	2716:2731	a new generation of GBR membrane	2716:2747	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	1	76	theme	oxide	171:175	arg1	CS					192:193	CS	192:193	CS	192:193	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	76	theme	oxide	171:175	arg1	used					246:249	used	246:249	being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	240:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	76	theme	oxide	171:175	arg1	-chitosan					181:189	graphene oxide (GO)-chitosan	162:189	graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	162:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	3	77	with	membrane	573:580	arg1	%					614:614	6.0%	611:614	6.0%	611:614	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	3	77	with	membrane	573:580	arg1	%					590:590	0.5%	587:590	0.5%	587:590	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	3	77	with	membrane	573:580	arg1	%					602:602	2.0%	599:602	2.0%	599:602	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	3	77	with	membrane	573:580	arg1	%					608:608	4.0%	605:608	4.0%	605:608	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	3	77	with	membrane	573:580	arg1	%					620:620	8.0% GO	617:623	8.0% GO	617:623	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	3	77	with	membrane	573:580	arg1	%					596:596	1.0%	593:596	1.0%	593:596	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	13	78	theme	composite	2231:2239	arg1	membrane					2241:2248	the composite membrane	2227:2248	the composite membrane	2227:2248	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	13	79	theme	GO	2089:2090	arg1	%					2105:2105	4.0%	2102:2105	4.0%	2102:2105	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	13	79	theme	GO	2089:2090	arg1	fraction					2077:2084	the mass fraction	2068:2084	the mass fraction of GO in CS	2068:2096	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	14	80	theme	membrane	2355:2362	arg1	group					2364:2368	the pure CS membrane group	2343:2368	the pure CS membrane group	2343:2368	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	4	81	theme	mechanical	707:716	arg1	machine					736:742	mechanical universal testing machine	707:742	mechanical universal testing machine (n=6)	707:748	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	4	81	theme	mechanical	707:716	arg1	n=6					745:747	n=6	745:747	n=6	745:747	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	15	82	theme	composite	2533:2541	arg1	membrane					2543:2550	the composite membrane	2529:2550	the composite membrane	2529:2550	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	10	83	theme	cell	1612:1615	arg1	counts					1617:1622	cell counts	1612:1622	cell counts	1612:1622	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	16	84	theme	membrane	2740:2747	arg1	generation					2722:2731	a new generation	2716:2731	a new generation of GBR membrane	2716:2747	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	1	85	theme	-chitosan	181:189	arg1	feasibility					147:157	the feasibility	143:157	the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	143:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	10	86	with	group	1645:1649	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	86	with	group	1645:1649	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	87	theme	layer	1900:1904	arg1	structure					1906:1914	an ordered layer structure	1889:1914	an ordered layer structure	1889:1914	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	9	88	theme	immunocytochemical	1525:1542	arg1	methods					1553:1559	immunocytochemical staining methods	1525:1559	immunocytochemical staining methods	1525:1559	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	1	89	dep	guided	196:201	arg1	used					246:249	used	246:249	being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	240:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	89	dep	guided	196:201	arg1	-chitosan					181:189	graphene oxide (GO)-chitosan	162:189	graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF)	162:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	10	90	theme	blank	1631:1635	arg1	group					1645:1649	the blank control group	1627:1649	the blank control group	1627:1649	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	91	with	groups	1761:1766	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	91	with	groups	1761:1766	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	15	92	theme	mass	2494:2497	arg1	%					2526:2526	4.0%	2523:2526	4.0%	2523:2526	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	15	92	theme	mass	2494:2497	arg1	fraction					2499:2506	the mass fraction	2490:2506	the mass fraction of GO in CS	2490:2518	Conclusions: When the mass fraction of GO in CS is 4.0%, the composite membrane has the best tensile strength.
31091565	1	93	theme	human	371:375	arg1	HGF					399:401	HGF	399:401	HGF	399:401	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	93	theme	human	371:375	arg1	fibroblasts					386:396	human gingival fibroblasts	371:396	human gingival fibroblasts (HGF)	371:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	3	94	theme	Composite	563:571	arg1	membrane					573:580	Composite membrane	563:580	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS	563:629	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	5	95	theme	composite	777:785	arg1	membrane					787:794	the composite membrane	773:794	the composite membrane	773:794	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	14	96	theme	significant	2298:2308	arg1	difference					2310:2319	no significant difference	2295:2319	no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group	2295:2407	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	14	97	theme	membrane	2394:2401	arg1	group					2403:2407	the GO/CS composite membrane group	2374:2407	the GO/CS composite membrane group	2374:2407	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	1	98	theme	fibroblasts	386:396	arg1	proliferation					354:366	the proliferation	350:366	the proliferation of human gingival fibroblasts (HGF)	350:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	9	99	theme	Surgery	1320:1326	arg1	Department					1283:1292	the Department	1279:1292	the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University	1279:1387	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	16	100	theme	good	2613:2616	arg1	cytocompatibility					2618:2634	good cytocompatibility	2613:2634	good cytocompatibility	2613:2634	The composite membrane showes good cytocompatibility, which lays a foundation for further in vivo experiments and the development of a new generation of GBR membrane.
31091565	11	101	theme	composite	1981:1989	arg1	membrane					1991:1998	the composite membrane	1977:1998	the composite membrane	1977:1998	X-ray diffraction analysis showed that GO could be found in the composite membrane.
31091565	7	102	theme	composite	1076:1084	arg1	membrane					1086:1093	the highest tensile strength composite membrane	1047:1093	the highest tensile strength composite membrane	1047:1093	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	0	103	theme	regeneration	87:98	arg1	membrane					110:117	bone regeneration composite membrane	82:117	bone regeneration composite membrane	82:117	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	3	104	dep	%	620:620	arg1	GO					622:623	GO	622:623	GO	622:623	Composite membrane with 0.5%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0% GO in CS was prepared.
31091565	12	105	theme	ratio	2056:2060	arg1	increase					2041:2048	the increase	2037:2048	the increase of GO ratio	2037:2060	The tensile strength increased with the increase of GO ratio.
31091565	5	106	with	microstructure	755:768	arg1	strength					818:825	the best tensile strength	801:825	the best tensile strength	801:825	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	7	107	theme	strength	1067:1074	arg1	membrane					1086:1093	the highest tensile strength composite membrane	1047:1093	the highest tensile strength composite membrane	1047:1093	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	14	108	theme	HGF	2269:2271	arg1	test					2261:2264	The CCK-8 test	2251:2264	The CCK-8 test of HGF	2251:2271	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	8	109	theme	group	1195:1199	arg1	strength					1175:1182	The tensile strength	1163:1182	The tensile strength of the new group of samples	1163:1210	The tensile strength of the new group of samples was tested.
31091565	10	110	theme	h.	1809:1810	arg1	Results					1812:1818	48 h. Results	1806:1818	48 h. Results	1806:1818	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	1	111	from	effect	340:345	arg1	proliferation					354:366	the proliferation	350:366	the proliferation of human gingival fibroblasts (HGF)	350:402	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	6	112	used	used	932:935	arg2	diffraction					916:926	X-ray diffraction	910:926	X-ray diffraction	910:926	X-ray diffraction was used to characterize the ingredients of the material.
31091565	8	113	theme	samples	1204:1210	arg1	samples					1204:1210	samples	1204:1210	samples	1204:1210	The tensile strength of the new group of samples was tested.
31091565	8	113	theme	samples	1204:1210	arg1	group					1195:1199	the new group	1187:1199	the new group of samples	1187:1210	The tensile strength of the new group of samples was tested.
31091565	13	114	from	CS	2095:2096	arg1	%					2105:2105	4.0%	2102:2105	4.0%	2102:2105	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	13	114	from	CS	2095:2096	arg1	fraction					2077:2084	the mass fraction	2068:2084	the mass fraction of GO in CS	2068:2096	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	7	115	theme	highest	1051:1057	arg1	membrane					1086:1093	the highest tensile strength composite membrane	1047:1093	the highest tensile strength composite membrane	1047:1093	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	14	116	theme	blank	2442:2446	arg1	P>0.05					2463:2468	P>0.05	2463:2468	P>0.05	2463:2468	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	14	116	theme	blank	2442:2446	arg1	group					2456:2460	the blank control group	2438:2460	the blank control group (P>0.05)	2438:2469	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	0	117	theme	cell	27:30	arg1	compatibility					32:44	cell compatibility	27:44	cell compatibility	27:44	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	1	118	theme	new	256:258	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	118	theme	new	256:258	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	5	119	theme	best	805:808	arg1	strength					818:825	the best tensile strength	801:825	the best tensile strength	801:825	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	11	120	theme	diffraction	1923:1933	arg1	analysis					1935:1942	X-ray diffraction analysis	1917:1942	X-ray diffraction analysis	1917:1942	X-ray diffraction analysis showed that GO could be found in the composite membrane.
31091565	1	121	theme	bone	267:270	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	121	theme	bone	267:270	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	122	used	used	246:249	arg2	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	122	used	used	246:249	arg2	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	5	123	theme	transmission	876:887	arg1	microscopy					898:907	transmission electron microscopy	876:907	transmission electron microscopy	876:907	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	1	124	theme	bone	203:206	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	124	theme	bone	203:206	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	0	125	theme	chitosan-graphene	51:67	arg1	oxide					69:73	a chitosan-graphene oxide	49:73	a chitosan-graphene oxide	49:73	[Mechanical properties and cell compatibility of a chitosan-graphene oxide guided bone regeneration composite membrane].
31091565	9	126	theme	primary	1452:1458	arg1	HGF					1460:1462	primary HGF	1452:1462	primary HGF	1452:1462	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	10	127	with	group	1673:1677	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	127	with	group	1673:1677	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	4	128	theme	membranes	683:691	arg1	strength					657:664	The tensile strength	645:664	The tensile strength of the composite membranes	645:691	The tensile strength of the composite membranes was tested by mechanical universal testing machine (n=6).
31091565	2	129	dep	solution	437:444	arg1	GO					434:435	GO	434:435	GO	434:435	Methods: The CS solution and GO solution were mixed in following ratios, ultrasonically crushed and dispersed, and formed into membranes by self-evaporation.
31091565	5	130	theme	scanning	843:850	arg1	microscopy					861:870	scanning electron microscopy	843:870	scanning electron microscopy	843:870	The microstructure of the composite membrane with the best tensile strength was observed by scanning electron microscopy and transmission electron microscopy.
31091565	7	131	theme	hydroxide	1126:1134	arg1	solution					1136:1143	a sodium hydroxide solution	1117:1143	a sodium hydroxide solution for acid removal	1117:1160	A group of samples was prepared again with the proportion of the highest tensile strength composite membrane, and were immersed in a sodium hydroxide solution for acid removal.
31091565	10	132	theme	composite	1688:1696	arg1	group					1707:1711	the composite membrane group	1684:1711	the composite membrane group with the highest tensile strength (n=5)	1684:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	9	133	dep	Hospital	1342:1349	arg1	University					1378:1387	University	1378:1387	University	1378:1387	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	6	134	theme	material	976:983	arg1	ingredients					957:967	the ingredients	953:967	the ingredients of the material	953:983	X-ray diffraction was used to characterize the ingredients of the material.
31091565	9	135	theme	impacted	1244:1251	arg1	teeth					1253:1257	The newly extracted impacted teeth	1224:1257	The newly extracted impacted teeth	1224:1257	The newly extracted impacted teeth were collected from the Department of Oral and Maxillofacial Surgery, Stomatologic Hospital and College, Anhui Medical University, and the gingival tissues remained on the teeth were taken for primary HGF and were cultured to P2 generation, which were identified by immunocytochemical staining methods.
31091565	14	136	theme	GO/CS	2378:2382	arg1	group					2403:2407	the GO/CS composite membrane group	2374:2407	the GO/CS composite membrane group	2374:2407	The CCK-8 test of HGF showed that there was no significant difference in absorbance between the pure CS membrane group and the GO/CS composite membrane group when they were compared with the blank control group (P>0.05).
31091565	10	137	theme	tensile	1730:1736	arg1	n=5					1748:1750	n=5	1748:1750	n=5	1748:1750	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	10	137	theme	tensile	1730:1736	arg1	strength					1738:1745	the highest tensile strength	1718:1745	the highest tensile strength (n=5)	1718:1751	Cell counting kit-8 (CCK-8) was applied to detect cell counts in the blank control group, the pure CS membrane group, and the composite membrane group with the highest tensile strength (n=5) and all groups co-cultured with the HGF for 24 h and 48 h. Results: After adding GO, the cross-section of the composite membrane was in an ordered layer structure.
31091565	13	138	from	fraction	2077:2084	arg1	CS					2095:2096	CS	2095:2096	CS	2095:2096	When the mass fraction of GO in CS was 4.0%, the tensile strength reached (134.8±7.3) MPa and the tensile strength reached (144.6±8.1) MPa after deacidification of the composite membrane.
31091565	1	139	theme	composite	221:229	arg1	membrane					291:298	a new guided bone regeneration (GBR) membrane	254:298	a new guided bone regeneration (GBR) membrane	254:298	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31091565	1	139	theme	composite	221:229	arg1	membrane					231:238	bone regeneration composite membrane	203:238	bone regeneration composite membrane	203:238	Objective: To explore the feasibility of graphene oxide (GO)-chitosan (CS) guided bone regeneration composite membrane being used as a new guided bone regeneration (GBR) membrane by testing its tensile strength and its effect on the proliferation of human gingival fibroblasts (HGF).
31812599	2	0	theme	faster	407:412	arg1	rate					425:428	a faster adsorption rate	405:428	a faster adsorption rate	405:428	From the results, CB (52%) exhibited superior removal efficiency compared to that of biochar (12%) and a faster adsorption rate.
31812599	4	1	theme	acid-base	925:933	arg1	reactions					935:943	acid-base reactions	925:943	acid-base reactions	925:943	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	4	2	theme	pore	811:814	arg1	distribution					821:832	pore size distribution	811:832	pore size distribution	811:832	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	5	3	theme	traditional	1000:1010	arg1	carbon					1032:1037	traditional adsorbent activated carbon	1000:1037	traditional adsorbent activated carbon	1000:1037	Moreover, compared to traditional adsorbent activated carbon, CB exhibited superior removal efficiency and cost-effectiveness.
31812599	1	4	theme	dissolved	162:170	arg1	DOM					188:190	DOM	188:190	DOM	188:190	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	4	theme	dissolved	162:170	arg1	matter					180:185	dissolved organic matter	162:185	dissolved organic matter (DOM)	162:191	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	5	5	theme	activated	1022:1030	arg1	carbon					1032:1037	traditional adsorbent activated carbon	1000:1037	traditional adsorbent activated carbon	1000:1037	Moreover, compared to traditional adsorbent activated carbon, CB exhibited superior removal efficiency and cost-effectiveness.
31812599	6	6	theme	advanced	1181:1188	arg1	treatment					1194:1202	advanced DOM treatment	1181:1202	advanced DOM treatment of BTCW	1181:1210	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	3	7	theme	distribution	479:490	arg1	Analysis					431:438	Analysis	431:438	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions	431:542	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	0	8	theme	chitosan-modified	76:92	arg1	biochar					94:100	chitosan-modified biochar	76:100	chitosan-modified biochar	76:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	4	9	from	change	801:806	arg1	groups					861:866	functional groups	850:866	functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions	850:975	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	4	9	from	change	801:806	arg1	distribution					821:832	pore size distribution	811:832	pore size distribution	811:832	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	4	10	theme	size	816:819	arg1	distribution					821:832	pore size distribution	811:832	pore size distribution	811:832	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	3	11	theme	molecular	462:470	arg1	distribution					479:490	molecular weight distribution	462:490	molecular weight distribution	462:490	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	1	12	theme	reusable	244:251	arg1	chitosan-biochar					266:281	a reusable and low-cost chitosan-biochar	242:281	a reusable and low-cost chitosan-biochar (CB)	242:286	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	12	theme	reusable	244:251	arg1	CB					284:285	CB	284:285	CB	284:285	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	0	13	theme	Adsorption	103:112	arg1	fractions					114:122	Adsorption fractions	103:122	Adsorption fractions	103:122	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	5	14	theme	superior	1053:1060	arg1	efficiency					1070:1079	superior removal efficiency	1053:1079	superior removal efficiency	1053:1079	Moreover, compared to traditional adsorbent activated carbon, CB exhibited superior removal efficiency and cost-effectiveness.
31812599	3	15	theme	DOM	447:449	arg1	fractions					451:459	the DOM fractions	443:459	the DOM fractions	443:459	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	1	16	theme	organic	172:178	arg1	DOM					188:190	DOM	188:190	DOM	188:190	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	16	theme	organic	172:178	arg1	matter					180:185	dissolved organic matter	162:185	dissolved organic matter (DOM)	162:191	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	5	17	theme	removal	1062:1068	arg1	efficiency					1070:1079	superior removal efficiency	1053:1079	superior removal efficiency	1053:1079	Moreover, compared to traditional adsorbent activated carbon, CB exhibited superior removal efficiency and cost-effectiveness.
31812599	3	18	theme	fractions	451:459	arg1	Analysis					431:438	Analysis	431:438	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions	431:542	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	0	19	theme	organic	10:16	arg1	matter					18:23	organic matter	10:23	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar	0:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	0	19	theme	organic	10:16	arg1	DOM					26:28	DOM	26:28	DOM	26:28	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	0	20	theme	Dissolved	0:8	arg1	removal					31:37	Dissolved organic matter (DOM) removal	0:37	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar	0:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	3	21	theme	DOM	600:602	arg1	substances					634:643	hydrophilic substances	622:643	hydrophilic substances	622:643	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	3	21	theme	DOM	600:602	arg1	components					604:613	DOM components	600:613	DOM components (e.g., hydrophilic substances)	600:644	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	3	22	theme	fluorescent	493:503	arg1	components					505:514	fluorescent components	493:514	fluorescent components	493:514	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	1	23	theme	low-cost	257:264	arg1	chitosan-biochar					266:281	a reusable and low-cost chitosan-biochar	242:281	a reusable and low-cost chitosan-biochar (CB)	242:286	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	23	theme	low-cost	257:264	arg1	CB					284:285	CB	284:285	CB	284:285	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	3	24	theme	weight	472:477	arg1	distribution					479:490	molecular weight distribution	462:490	molecular weight distribution	462:490	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	0	25	theme	matter	18:23	arg1	removal					31:37	Dissolved organic matter (DOM) removal	0:37	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar	0:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	4	26	theme	removal	711:717	arg1	characteristics					719:733	removal characteristics	711:733	removal characteristics	711:733	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	3	27	theme	more	586:589	arg1	kinds					591:595	more kinds	586:595	more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar	586:674	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	0	28	dep	fractions	114:122	arg1	removal					31:37	Dissolved organic matter (DOM) removal	0:37	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar	0:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	3	29	dep	substances	634:643	arg1	e.g.					616:619	e.g.	616:619	e.g.	616:619	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	4	30	theme	bonding	881:887	arg1	sites					889:893	bonding sites	881:893	bonding sites for DOM	881:901	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	0	31	from	wastewater	62:71	arg1	removal					31:37	Dissolved organic matter (DOM) removal	0:37	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar	0:100	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	4	32	theme	adsorption	759:768	arg1	efficiency					770:779	the adsorption efficiency	755:779	the adsorption efficiency	755:779	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	1	33	theme	actual	198:203	arg1	BTCW					235:238	BTCW	235:238	BTCW	235:238	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	33	theme	actual	198:203	arg1	wastewater					223:232	actual biotreated coking wastewater	198:232	actual biotreated coking wastewater (BTCW)	198:239	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	2	34	theme	superior	339:346	arg1	efficiency					356:365	superior removal efficiency	339:365	superior removal efficiency	339:365	From the results, CB (52%) exhibited superior removal efficiency compared to that of biochar (12%) and a faster adsorption rate.
31812599	4	35	theme	material	681:688	arg1	characterization					690:705	The material characterization	677:705	The material characterization	677:705	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	3	36	theme	molecular	521:529	arg1	compositions					531:542	molecular compositions	521:542	molecular compositions	521:542	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	1	37	theme	biotreated	205:214	arg1	BTCW					235:238	BTCW	235:238	BTCW	235:238	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	37	theme	biotreated	205:214	arg1	wastewater					223:232	actual biotreated coking wastewater	198:232	actual biotreated coking wastewater (BTCW)	198:239	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	4	38	theme	electrostatic	950:962	arg1	interactions					964:975	electrostatic interactions	950:975	electrostatic interactions	950:975	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	0	39	theme	biotreated	44:53	arg1	wastewater					62:71	biotreated coking wastewater	44:71	biotreated coking wastewater	44:71	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	3	40	theme	components	505:514	arg1	Analysis					431:438	Analysis	431:438	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions	431:542	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	6	41	theme	potential	1145:1153	arg1	CB					1137:1138	CB	1137:1138	CB	1137:1138	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	6	41	theme	potential	1145:1153	arg1	alternative					1155:1165	a potential alternative	1143:1165	a potential alternative adsorbent for advanced DOM treatment of BTCW	1143:1210	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	3	42	theme	components	604:613	arg1	kinds					591:595	more kinds	586:595	more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar	586:674	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	4	43	theme	hydrogen	907:914	arg1	bonding					916:922	hydrogen bonding	907:922	hydrogen bonding	907:922	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	5	44	theme	adsorbent	1012:1020	arg1	carbon					1032:1037	traditional adsorbent activated carbon	1000:1037	traditional adsorbent activated carbon	1000:1037	Moreover, compared to traditional adsorbent activated carbon, CB exhibited superior removal efficiency and cost-effectiveness.
31812599	4	45	theme	functional	850:859	arg1	groups					861:866	functional groups	850:866	functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions	850:975	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	6	46	theme	DOM	1190:1192	arg1	treatment					1194:1202	advanced DOM treatment	1181:1202	advanced DOM treatment of BTCW	1181:1210	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	6	47	theme	BTCW	1207:1210	arg1	treatment					1194:1202	advanced DOM treatment	1181:1202	advanced DOM treatment of BTCW	1181:1210	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	1	48	theme	coking	216:221	arg1	BTCW					235:238	BTCW	235:238	BTCW	235:238	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	1	48	theme	coking	216:221	arg1	wastewater					223:232	actual biotreated coking wastewater	198:232	actual biotreated coking wastewater (BTCW)	198:239	To effectively remove dissolved organic matter (DOM) from actual biotreated coking wastewater (BTCW), a reusable and low-cost chitosan-biochar (CB) was prepared.
31812599	4	49	from	increase	838:845	arg1	groups					861:866	functional groups	850:866	functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions	850:975	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	4	49	from	increase	838:845	arg1	distribution					821:832	pore size distribution	811:832	pore size distribution	811:832	The material characterization and removal characteristics jointly proved that the adsorption efficiency was promoted by the change in pore size distribution and increase in functional groups that provide bonding sites for DOM via hydrogen bonding, acid-base reactions, and electrostatic interactions.
31812599	3	50	theme	chitosan	559:566	arg1	modification					568:579	chitosan modification	559:579	chitosan modification	559:579	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	0	51	theme	coking	55:60	arg1	wastewater					62:71	biotreated coking wastewater	44:71	biotreated coking wastewater	44:71	Dissolved organic matter (DOM) removal from biotreated coking wastewater by chitosan-modified biochar: Adsorption fractions and mechanisms.
31812599	2	52	theme	removal	348:354	arg1	efficiency					356:365	superior removal efficiency	339:365	superior removal efficiency	339:365	From the results, CB (52%) exhibited superior removal efficiency compared to that of biochar (12%) and a faster adsorption rate.
31812599	3	53	theme	compositions	531:542	arg1	Analysis					431:438	Analysis	431:438	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions	431:542	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	3	54	with	affinity	654:661	arg1	biochar					668:674	biochar	668:674	biochar	668:674	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	2	55	theme	adsorption	414:423	arg1	rate					425:428	a faster adsorption rate	405:428	a faster adsorption rate	405:428	From the results, CB (52%) exhibited superior removal efficiency compared to that of biochar (12%) and a faster adsorption rate.
31812599	3	56	theme	hydrophilic	622:632	arg1	substances					634:643	hydrophilic substances	622:643	hydrophilic substances	622:643	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	3	56	theme	hydrophilic	622:632	arg1	components					604:613	DOM components	600:613	DOM components (e.g., hydrophilic substances)	600:644	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
31812599	6	57	theme	adsorbent	1167:1175	arg1	CB					1137:1138	CB	1137:1138	CB	1137:1138	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	6	57	theme	adsorbent	1167:1175	arg1	alternative					1155:1165	a potential alternative	1143:1165	a potential alternative adsorbent for advanced DOM treatment of BTCW	1143:1210	These results demonstrated that CB is a potential alternative adsorbent for advanced DOM treatment of BTCW.
31812599	3	58	dep	kinds	591:595	arg1	have					646:649	have	646:649	have an affinity with biochar	646:674	Analysis of the DOM fractions, molecular weight distribution, fluorescent components, and molecular compositions indicated that chitosan modification made more kinds of DOM components (e.g., hydrophilic substances) have an affinity with biochar.
30974136	0	0	theme	composite	72:80	arg1	hydrogel					82:89	Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel	34:89	Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel	34:89	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	6	1	theme	wound	1222:1226	arg1	dressing					1228:1235	a wound dressing	1220:1235	a wound dressing	1220:1235	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	1	2	theme	cutaneous	147:155	arg1	healing					163:169	cutaneous wound healing	147:169	cutaneous wound healing process	147:177	Wound dressing is of significant importance to promote cutaneous wound healing process.
30974136	0	3	theme	/Dextran-aldehyde	54:70	arg1	hydrogel					82:89	Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel	34:89	Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel	34:89	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	1	4	theme	wound	157:161	arg1	healing					163:169	cutaneous wound healing	147:169	cutaneous wound healing process	147:177	Wound dressing is of significant importance to promote cutaneous wound healing process.
30974136	4	5	with	structure	589:597	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	6	theme	low	865:867	arg1	hemolysis					869:877	very low hemolysis	860:877	very low hemolysis	860:877	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	2	7	theme	vinyl	265:269	arg1	alcohol					271:277	vinyl alcohol	265:277	vinyl alcohol	265:277	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	4	8	theme	water	748:752	arg1	vapor					754:758	suitable water vapor	739:758	suitable water vapor	739:758	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	9	theme	absorb	701:706	arg1	fluid					708:712	absorb fluid	701:712	absorb fluid of 6 time	701:722	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	6	10	contain	have	1245:1248	arg2	potential					1250:1258	potential	1250:1258	potential	1250:1258	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	6	10	contain	have	1245:1248	arg1	suitable					1192:1199	suitable	1192:1199	suitable	1192:1199	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	6	10	contain	have	1245:1248	arg1	hydrogel					1179:1186	the PVA/DA hydrogel	1168:1186	the PVA/DA hydrogel	1168:1186	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	5	11	theme	healing	909:915	arg1	tests					917:921	wound healing tests	903:921	wound healing tests using a full-thickness skin wound model	903:961	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	4	12	dep	3-dimensional	560:572	arg1	porous					582:587	porous	582:587	porous	582:587	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	6	13	theme	biomedical	1279:1288	arg1	applications					1290:1301	various biomedical applications	1271:1301	various biomedical applications	1271:1301	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	4	14	theme	5-10 μm	635:641	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	5	15	theme	wound	903:907	arg1	healing					909:915	wound healing	903:915	wound healing tests using a full-thickness skin wound model	903:961	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	1	16	theme	healing	163:169	arg1	process					171:177	cutaneous wound healing process	147:177	cutaneous wound healing process	147:177	Wound dressing is of significant importance to promote cutaneous wound healing process.
30974136	4	17	theme	transmission	760:771	arg1	rate					773:776	transmission rate	760:776	transmission rate	760:776	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	3	18	theme	mechanical	436:445	arg1	tests					456:460	mechanical property tests	436:460	mechanical property tests	436:460	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	0	19	theme	wound	10:14	arg1	dressing					16:23	Effective wound dressing	0:23	Effective wound dressing	0:23	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	4	20	theme	biocompatibility	834:849	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	2	21	theme	solution	297:304	arg1	blend					306:310	a Poly (vinyl alcohol)/Dextran-aldehyde solution blend	257:310	a Poly (vinyl alcohol)/Dextran-aldehyde solution blend	257:310	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	5	22	theme	skin	1156:1159	arg1	regeneration					1140:1151	a faster regeneration	1131:1151	a faster regeneration of skin	1131:1159	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	3	23	theme	infrared	413:420	arg1	spectroscopy					422:433	infrared spectroscopy	413:433	infrared spectroscopy	413:433	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	0	24	theme	Effective	0:8	arg1	dressing					16:23	Effective wound dressing	0:23	Effective wound dressing	0:23	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	4	25	theme	good	829:832	arg1	biocompatibility					834:849	good biocompatibility	829:849	good biocompatibility shown by very low hemolysis and no cytotoxicity	829:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	0	26	dep	/Dextran-aldehyde	54:70	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	4	27	theme	5.6 MPa	671:677	arg1	5-10 μm					635:641	5-10 μm	635:641	5-10 μm	635:641	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	27	theme	5.6 MPa	671:677	arg1	biocompatibility					834:849	good biocompatibility	829:849	good biocompatibility shown by very low hemolysis and no cytotoxicity	829:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	27	theme	5.6 MPa	671:677	arg1	ability					690:696	efficient ability	680:696	efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment	680:823	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	27	theme	5.6 MPa	671:677	arg1	strength					659:666	strong tensile strength	644:666	strong tensile strength of 5.6 MPa	644:677	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	1	28	theme	Wound	92:96	arg1	dressing					98:105	Wound dressing	92:105	Wound dressing	92:105	Wound dressing is of significant importance to promote cutaneous wound healing process.
30974136	5	29	theme	skin	946:949	arg1	model					957:961	a full-thickness skin wound model	929:961	a full-thickness skin wound model	929:961	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	2	30	theme	/Dextran-aldehyde	279:295	arg1	blend					306:310	a Poly (vinyl alcohol)/Dextran-aldehyde solution blend	257:310	a Poly (vinyl alcohol)/Dextran-aldehyde solution blend	257:310	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	5	31	theme	full-thickness	931:944	arg1	model					957:961	a full-thickness skin wound model	929:961	a full-thickness skin wound model	929:961	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	3	32	theme	swelling	463:470	arg1	test					481:484	swelling behavior test	463:484	swelling behavior test	463:484	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	4	33	theme	3-dimensional	560:572	arg1	structure					589:597	a 3-dimensional, highly porous structure	558:597	a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	558:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	34	theme	tensile	651:657	arg1	strength					659:666	strong tensile strength	644:666	strong tensile strength of 5.6 MPa	644:677	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	2	35	theme	PVA/DA	222:227	arg1	hydrogel					229:236	a PVA/DA hydrogel	220:236	a PVA/DA hydrogel	220:236	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	6	36	theme	PVA/DA	1172:1177	arg1	suitable					1192:1199	suitable	1192:1199	suitable	1192:1199	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	6	36	theme	PVA/DA	1172:1177	arg1	hydrogel					1179:1186	the PVA/DA hydrogel	1168:1186	the PVA/DA hydrogel	1168:1186	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	3	37	theme	property	447:454	arg1	tests					456:460	mechanical property tests	436:460	mechanical property tests	436:460	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	5	38	theme	faster	1133:1138	arg1	regeneration					1140:1151	a faster regeneration	1131:1151	a faster regeneration of skin	1131:1159	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	4	39	theme	suitable	739:746	arg1	vapor					754:758	suitable water vapor	739:758	suitable water vapor	739:758	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	0	40	theme	vinyl	40:44	arg1	alcohol					46:52	vinyl alcohol	40:52	vinyl alcohol	40:52	Effective wound dressing based on Poly (vinyl alcohol)/Dextran-aldehyde composite hydrogel.
30974136	4	41	theme	moist	807:811	arg1	environment					813:823	a moist environment	805:823	a moist environment	805:823	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	5	42	theme	histological	1099:1110	arg1	analysis					1112:1119	histological analysis	1099:1119	histological analysis	1099:1119	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	2	43	theme	wound	204:208	arg1	dressing					210:217	an effective wound dressing	191:217	an effective wound dressing	191:217	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	5	44	theme	PVA/DA	1026:1031	arg1	hydrogel					1033:1040	the PVA/DA hydrogel	1022:1040	the PVA/DA hydrogel	1022:1040	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	3	45	theme	biocompatibility	490:505	arg1	test					507:510	biocompatibility test	490:510	biocompatibility test	490:510	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	4	46	theme	PVA/DA	537:542	arg1	hydrogels					544:552	the PVA/DA hydrogels	533:552	the PVA/DA hydrogels	533:552	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	6	47	theme	various	1271:1277	arg1	applications					1290:1301	various biomedical applications	1271:1301	various biomedical applications	1271:1301	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	2	48	theme	effective	194:202	arg1	dressing					210:217	an effective wound dressing	191:217	an effective wound dressing	191:217	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	5	49	theme	macroscopic	964:974	arg1	observations					976:987	macroscopic observations	964:987	macroscopic observations	964:987	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	4	50	theme	efficient	680:688	arg1	ability					690:696	efficient ability	680:696	efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment	680:823	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	1	51	theme	significant	113:123	arg1	importance					125:134	significant importance	113:134	significant importance	113:134	Wound dressing is of significant importance to promote cutaneous wound healing process.
30974136	4	52	contain	had	554:556	arg1	hydrogels					544:552	the PVA/DA hydrogels	533:552	the PVA/DA hydrogels	533:552	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	52	contain	had	554:556	arg2	structure					589:597	a 3-dimensional, highly porous structure	558:597	a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	558:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	53	theme	distributed	614:624	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	54	theme	time	719:722	arg1	fluid					708:712	absorb fluid	701:712	absorb fluid of 6 time	701:722	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	5	55	theme	complete	1057:1064	arg1	healing					1066:1072	complete healing	1057:1072	complete healing	1057:1072	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	2	56	dep	/Dextran-aldehyde	279:295	arg1	alcohol					271:277	vinyl alcohol	265:277	vinyl alcohol	265:277	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
30974136	3	57	theme	behavior	472:479	arg1	test					481:484	swelling behavior test	463:484	swelling behavior test	463:484	We characterized the hydrogel by infrared spectroscopy, mechanical property tests, swelling behavior test and biocompatibility test.
30974136	4	58	dep	weight	728:733	arg1	rate					773:776	transmission rate	760:776	transmission rate	760:776	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	59	theme	strong	644:649	arg1	strength					659:666	strong tensile strength	644:666	strong tensile strength of 5.6 MPa	644:677	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	60	theme	m-2day-1	788:795	arg1	vapor					754:758	suitable water vapor	739:758	suitable water vapor	739:758	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	60	theme	m-2day-1	788:795	arg1	weight					728:733	its weight	724:733	its weight	724:733	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	61	theme	ability	690:696	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	4	62	theme	strength	659:666	arg1	pores					626:630	uniformly distributed pores	604:630	uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity	604:897	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	6	63	from	use	1264:1266	arg1	applications					1290:1301	various biomedical applications	1271:1301	various biomedical applications	1271:1301	Thus, the PVA/DA hydrogel was suitable for application as a wound dressing and may have potential for use in various biomedical applications.
30974136	5	64	theme	wound	951:955	arg1	model					957:961	a full-thickness skin wound model	929:961	a full-thickness skin wound model	929:961	In wound healing tests using a full-thickness skin wound model, macroscopic observations showed that the wound covered by the PVA/DA hydrogel almost reached complete healing faster by 10 days, while histological analysis indicated a faster regeneration of skin.
30974136	4	65	theme	g	786:786	arg1	m-2day-1					788:795	2100 g m-2day-1	781:795	2100 g m-2day-1	781:795	Results showed that the PVA/DA hydrogels had a 3-dimensional, highly porous structure with uniformly distributed pores of 5-10 μm, strong tensile strength of 5.6 MPa, efficient ability to absorb fluid of 6 time its weight and suitable water vapor transmission rate of 2100 g m-2day-1 to keep a moist environment and good biocompatibility shown by very low hemolysis and no cytotoxicity.
30974136	2	66	theme	freeze-thaw	342:352	arg1	method					354:359	freeze-thaw method	342:359	freeze-thaw method	342:359	To develop an effective wound dressing, a PVA/DA hydrogel was prepared using a Poly (vinyl alcohol)/Dextran-aldehyde solution blend, followed by crosslinking via freeze-thaw method and freeze-drying.
32010168	5	0	theme	double	774:779	arg1	mutants					804:810	double, triple, and quadruple mutants	774:810	double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes	774:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	6	1	theme	core	1044:1047	arg1	XylT1B					1069:1074	XylT1B	1069:1074	XylT1B	1069:1074	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	1	theme	core	1044:1047	arg1	xylosyltransferase					1049:1066	a second putative core xylosyltransferase	1026:1066	a second putative core xylosyltransferase (XylT1B)	1026:1075	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	1	2	theme	β1,2-core	156:164	arg1	residues					186:193	α1,3-fucose residues	174:193	α1,3-fucose residues	174:193	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	2	theme	β1,2-core	156:164	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	2	theme	β1,2-core	156:164	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	3	theme	methylated	247:256	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	3	theme	methylated	247:256	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	9	4	theme	mutant	1342:1347	arg1	strains					1349:1355	the mutant strains	1338:1355	the mutant strains generated	1338:1365	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	0	5	from	Insights	6:13	arg1	reinhardtii					70:80	C. reinhardtii	67:80	C. reinhardtii	67:80	Novel Insights Into N-Glycan Fucosylation and Core Xylosylation in C. reinhardtii.
32010168	8	6	theme	xylosyltransferases	1201:1219	arg1	knockdown					1183:1191	knockdown	1183:1191	knockdown of both xylosyltransferases and the fucosyltransferase	1183:1246	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	1	7	dep	reinhardtii	97:107	arg1	reinhardtii					113:123	C. reinhardtii	110:123	C. reinhardtii	110:123	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	3	8	from	existence	526:534	arg1	reinhardtii					578:588	C. reinhardtii	575:588	C. reinhardtii	575:588	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	6	9	from	compositions	909:920	arg1	strains					966:972	these mutant strains	953:972	these mutant strains	953:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	10	theme	N-glycopeptides	932:946	arg1	compositions					909:920	N-glycan compositions	900:920	N-glycan compositions of intact N-glycopeptides from these mutant strains	900:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	7	11	theme	fucosyltransferase	1130:1147	arg1	role					1111:1114	the role	1107:1114	the role of a putative fucosyltransferase	1107:1147	Additionally, the role of a putative fucosyltransferase was revealed.
32010168	8	12	with	strains	1170:1176	arg1	knockdown					1183:1191	knockdown	1183:1191	knockdown of both xylosyltransferases and the fucosyltransferase	1183:1246	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	3	13	theme	second	541:546	arg1	xylosyltransferase					553:570	a second core xylosyltransferase	539:570	a second core xylosyltransferase	539:570	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	3	14	theme	core	548:551	arg1	xylosyltransferase					553:570	a second core xylosyltransferase	539:570	a second core xylosyltransferase	539:570	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	6	15	theme	intact	925:930	arg1	N-glycopeptides					932:946	intact N-glycopeptides	925:946	intact N-glycopeptides from these mutant strains	925:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	3	16	located	found	443:447	arg1	mutant					474:479	a XylT1A/Man1A double mutant	452:479	a XylT1A/Man1A double mutant	452:479	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	3	16	located	found	443:447	arg2	N-Glycans					433:441	N-Glycans	433:441	N-Glycans found in a XylT1A/Man1A double mutant	433:479	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	3	17	theme	core	489:492	arg1	residues					501:508	core xylose residues	489:508	core xylose residues	489:508	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	9	18	theme	single	1419:1424	arg1	epitopes					1440:1447	single N-glycan core epitopes	1419:1447	single N-glycan core epitopes	1419:1447	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	4	19	theme	N-glycosylation	717:731	arg1	pathway					733:739	the N-glycosylation pathway	713:739	the N-glycosylation pathway	713:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	4	20	theme	genes	695:699	arg1	mutants					674:680	novel single knockdown mutants	651:680	novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway	651:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	9	21	theme	protein	1458:1464	arg1	function					1466:1473	protein function	1458:1473	protein function	1458:1473	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	5	22	theme	pathway	852:858	arg1	genes					860:864	already known N-glycosylation pathway genes	822:864	already known N-glycosylation pathway genes	822:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	4	23	theme	important	620:628	arg1	enzymes					612:618	enzymes	612:618	enzymes important for N-glycosylation	612:648	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	2	24	theme	recent	273:278	arg1	study					280:284	a recent study	271:284	a recent study	271:284	In a recent study, XylT1A was shown to act as core xylosyltransferase, whereby its action was of importance for an inhibition of excessive Man1A dependent trimming.
32010168	4	25	theme	single	657:662	arg1	mutants					674:680	novel single knockdown mutants	651:680	novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway	651:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	5	26	theme	quadruple	794:802	arg1	mutants					804:810	double, triple, and quadruple mutants	774:810	double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes	774:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	3	27	contain	carried	481:487	arg2	residues					501:508	core xylose residues	489:508	core xylose residues	489:508	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	3	27	contain	carried	481:487	arg1	N-Glycans					433:441	N-Glycans	433:441	N-Glycans found in a XylT1A/Man1A double mutant	433:479	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	6	28	theme	second	1028:1033	arg1	XylT1B					1069:1074	XylT1B	1069:1074	XylT1B	1069:1074	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	28	theme	second	1028:1033	arg1	xylosyltransferase					1049:1066	a second putative core xylosyltransferase	1026:1066	a second putative core xylosyltransferase (XylT1B)	1026:1075	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	2	29	theme	trimming	423:430	arg1	inhibition					383:392	an inhibition	380:392	an inhibition of excessive Man1A dependent trimming	380:430	In a recent study, XylT1A was shown to act as core xylosyltransferase, whereby its action was of importance for an inhibition of excessive Man1A dependent trimming.
32010168	1	30	theme	α1,3-fucose	174:184	arg1	residues					186:193	α1,3-fucose residues	174:193	α1,3-fucose residues	174:193	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	30	theme	α1,3-fucose	174:184	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	8	31	theme	fucosyltransferase	1229:1246	arg1	knockdown					1183:1191	knockdown	1183:1191	knockdown of both xylosyltransferases and the fucosyltransferase	1183:1246	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	6	32	theme	N-glycan	900:907	arg1	compositions					909:920	N-glycan compositions	900:920	N-glycan compositions of intact N-glycopeptides from these mutant strains	900:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	1	33	theme	Chlamydomonas	83:95	arg1	reinhardtii					97:107	Chlamydomonas reinhardtii	83:107	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans	83:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	6	34	from	strains	966:972	arg1	compositions					909:920	N-glycan compositions	900:920	N-glycan compositions of intact N-glycopeptides from these mutant strains	900:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	34	from	strains	966:972	arg1	N-glycopeptides					932:946	intact N-glycopeptides	925:946	intact N-glycopeptides from these mutant strains	925:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	8	35	theme	core	1312:1315	arg1	modifications					1317:1329	strongly diminished core modifications	1292:1329	strongly diminished core modifications	1292:1329	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	0	36	theme	Novel	0:4	arg1	Insights					6:13	Novel Insights	0:13	Novel Insights Into N-Glycan Fucosylation and Core Xylosylation in C. reinhardtii	0:80	Novel Insights Into N-Glycan Fucosylation and Core Xylosylation in C. reinhardtii.
32010168	1	37	theme	reinhardtii	97:107	arg1	N-glycans					126:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans	83:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans	83:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	4	38	theme	knockdown	664:672	arg1	mutants					674:680	novel single knockdown mutants	651:680	novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway	651:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	2	39	theme	dependent	413:421	arg1	trimming					423:430	excessive Man1A dependent trimming	397:430	excessive Man1A dependent trimming	397:430	In a recent study, XylT1A was shown to act as core xylosyltransferase, whereby its action was of importance for an inhibition of excessive Man1A dependent trimming.
32010168	6	40	theme	candidate	998:1006	arg1	gene					1008:1011	a candidate gene	996:1011	a candidate gene encoding for a second putative core xylosyltransferase (XylT1B)	996:1075	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	5	41	theme	N-glycosylation	836:850	arg1	genes					860:864	already known N-glycosylation pathway genes	822:864	already known N-glycosylation pathway genes	822:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	3	42	theme	xylosyltransferase	553:570	arg1	existence					526:534	the existence	522:534	the existence of a second core xylosyltransferase in C. reinhardtii	522:588	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	3	43	theme	xylose	494:499	arg1	residues					501:508	core xylose residues	489:508	core xylose residues	489:508	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	4	44	theme	candidate	685:693	arg1	genes					695:699	candidate genes	685:699	candidate genes involved in the N-glycosylation pathway	685:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	5	45	theme	triple	782:787	arg1	mutants					804:810	double, triple, and quadruple mutants	774:810	double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes	774:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	6	46	gly	N-glycopeptides	932:946	arg1	strains					966:972	these mutant strains	953:972	these mutant strains	953:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	46	gly	N-glycopeptides	932:946	arg2	N-glycopeptides					932:946	intact N-glycopeptides	925:946	intact N-glycopeptides from these mutant strains	925:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	8	47	theme	diminished	1301:1310	arg1	modifications					1317:1329	strongly diminished core modifications	1292:1329	strongly diminished core modifications	1292:1329	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	0	48	theme	N-Glycan	20:27	arg1	Fucosylation					29:40	N-Glycan Fucosylation	20:40	N-Glycan Fucosylation	20:40	Novel Insights Into N-Glycan Fucosylation and Core Xylosylation in C. reinhardtii.
32010168	1	49	theme	plant	207:211	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	49	theme	plant	207:211	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	8	50	theme	N-glycans	1277:1285	arg1	formation					1264:1272	the formation	1260:1272	the formation of N-glycans with strongly diminished core modifications	1260:1329	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	6	51	theme	mass	977:980	arg1	spectrometry					982:993	mass spectrometry	977:993	mass spectrometry	977:993	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	9	52	theme	further	1389:1395	arg1	investigations					1397:1410	further investigations	1389:1410	further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii	1389:1491	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	0	53	theme	Core	46:49	arg1	Xylosylation					51:62	Core Xylosylation	46:62	Core Xylosylation	46:62	Novel Insights Into N-Glycan Fucosylation and Core Xylosylation in C. reinhardtii.
32010168	9	54	theme	N-glycan	1426:1433	arg1	epitopes					1440:1447	single N-glycan core epitopes	1419:1447	single N-glycan core epitopes	1419:1447	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	6	55	theme	mutant	959:964	arg1	strains					966:972	these mutant strains	953:972	these mutant strains	953:972	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	7	56	theme	putative	1121:1128	arg1	fucosyltransferase					1130:1147	a putative fucosyltransferase	1119:1147	a putative fucosyltransferase	1119:1147	Additionally, the role of a putative fucosyltransferase was revealed.
32010168	2	57	theme	core	314:317	arg1	xylosyltransferase					319:336	core xylosyltransferase	314:336	core xylosyltransferase	314:336	In a recent study, XylT1A was shown to act as core xylosyltransferase, whereby its action was of importance for an inhibition of excessive Man1A dependent trimming.
32010168	9	58	theme	core	1435:1438	arg1	epitopes					1440:1447	single N-glycan core epitopes	1419:1447	single N-glycan core epitopes	1419:1447	Thus, the mutant strains generated will pave the way for further investigations on how single N-glycan core epitopes modulate protein function in C. reinhardtii.
32010168	3	59	theme	XylT1A/Man1A	454:465	arg1	mutant					474:479	a XylT1A/Man1A double mutant	452:479	a XylT1A/Man1A double mutant	452:479	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	1	60	theme	atypical	213:220	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	60	theme	atypical	213:220	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	3	61	theme	double	467:472	arg1	mutant					474:479	a XylT1A/Man1A double mutant	452:479	a XylT1A/Man1A double mutant	452:479	N-Glycans found in a XylT1A/Man1A double mutant carried core xylose residues, suggesting the existence of a second core xylosyltransferase in C. reinhardtii.
32010168	1	62	theme	terminal	222:229	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	62	theme	terminal	222:229	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	63	theme	plant	142:146	arg1	residues					186:193	α1,3-fucose residues	174:193	α1,3-fucose residues	174:193	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	63	theme	plant	142:146	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	63	theme	plant	142:146	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	4	64	theme	novel	651:655	arg1	mutants					674:680	novel single knockdown mutants	651:680	novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway	651:739	To further elucidate enzymes important for N-glycosylation, novel single knockdown mutants of candidate genes involved in the N-glycosylation pathway were characterized.
32010168	1	65	theme	β1,4-xylose	231:241	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	65	theme	β1,4-xylose	231:241	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	8	66	theme	Mutant	1163:1168	arg1	strains					1170:1176	Mutant strains	1163:1176	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase	1163:1246	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	1	67	contain	carry	136:140	arg1	N-glycans					126:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans	83:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans	83:134	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	67	contain	carry	136:140	arg2	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	67	contain	carry	136:140	arg2	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	67	contain	carry	136:140	arg2	residues					186:193	α1,3-fucose residues	174:193	α1,3-fucose residues	174:193	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	5	68	theme	known	830:834	arg1	genes					860:864	already known N-glycosylation pathway genes	822:864	already known N-glycosylation pathway genes	822:864	In addition, double, triple, and quadruple mutants affecting already known N-glycosylation pathway genes were generated.
32010168	8	69	with	formation	1264:1272	arg1	modifications					1317:1329	strongly diminished core modifications	1292:1329	strongly diminished core modifications	1292:1329	Mutant strains with knockdown of both xylosyltransferases and the fucosyltransferase resulted in the formation of N-glycans with strongly diminished core modifications.
32010168	1	70	theme	typical	148:154	arg1	residues					186:193	α1,3-fucose residues	174:193	α1,3-fucose residues	174:193	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	70	theme	typical	148:154	arg1	mannoses					258:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	plant atypical terminal β1,4-xylose and methylated mannoses	207:265	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	1	70	theme	typical	148:154	arg1	xylose					166:171	plant typical β1,2-core xylose	142:171	plant typical β1,2-core xylose	142:171	Chlamydomonas reinhardtii (C. reinhardtii) N-glycans carry plant typical β1,2-core xylose, α1,3-fucose residues, as well as plant atypical terminal β1,4-xylose and methylated mannoses.
32010168	6	71	theme	putative	1035:1042	arg1	XylT1B					1069:1074	XylT1B	1069:1074	XylT1B	1069:1074	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
32010168	6	71	theme	putative	1035:1042	arg1	xylosyltransferase					1049:1066	a second putative core xylosyltransferase	1026:1066	a second putative core xylosyltransferase (XylT1B)	1026:1075	By characterizing N-glycan compositions of intact N-glycopeptides from these mutant strains by mass spectrometry, a candidate gene encoding for a second putative core xylosyltransferase (XylT1B) was identified.
31899241	4	0	theme	whole	457:461	arg1	flour					469:473	whole grain flour	457:473	whole grain flour	457:473	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	7	1	theme	High	661:664	arg1	variability					666:676	High variability	661:676	High variability in pasting and thermal properties of flour	661:719	High variability in pasting and thermal properties of flour was observed between genotypes.
31899241	11	2	theme	genotype × environment	1348:1369	arg1	effects					1321:1327	lower effects	1315:1327	lower effects of environment and genotype × environment interaction	1315:1381	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	11	2	theme	genotype × environment	1348:1369	arg1	effect					1292:1297	higher effect	1285:1297	higher effect of genotype	1285:1309	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	3	3	theme	Half-sib	308:315	arg1	families					317:324	Half-sib families	308:324	Half-sib families	308:324	Half-sib families were selected from original germplasm and reproduced during 2014 and 2015.
31899241	9	4	theme	purple	951:956	arg1	genotypes					958:966	purple genotypes	951:966	purple genotypes	951:966	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	10	5	theme	retrogradation	1057:1070	arg1	enthalpy					1072:1079	The amylopectin retrogradation enthalpy	1041:1079	The amylopectin retrogradation enthalpy	1041:1079	The amylopectin retrogradation enthalpy negatively correlated with polyphenols (r = -0.35, P < 0.05) and anthocyanins (r = -0.40, P < 0.05), probably due to interactions formed after starch gelatinization.
31899241	6	6	theme	amylose	620:626	arg1	content					628:634	amylose content	620:634	amylose content	620:634	Composition of purple maize did not show significant differences with controls, but amylose content was significantly lower.
31899241	3	7	theme	original	345:352	arg1	germplasm					354:362	original germplasm	345:362	original germplasm	345:362	Half-sib families were selected from original germplasm and reproduced during 2014 and 2015.
31899241	11	8	theme	environment	1332:1342	arg1	effects					1321:1327	lower effects	1315:1327	lower effects of environment and genotype × environment interaction	1315:1381	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	11	8	theme	environment	1332:1342	arg1	effect					1292:1297	higher effect	1285:1297	higher effect of genotype	1285:1309	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	6	9	with	differences	589:599	arg1	controls					606:613	controls	606:613	controls	606:613	Composition of purple maize did not show significant differences with controls, but amylose content was significantly lower.
31899241	8	10	theme	granules	878:885	arg1	fragility					887:895	starch granules fragility	871:895	starch granules fragility	871:895	Anthocyanin content positively correlated with breakdown (r = 0.37, P < 0.05), indicating that anthocyanins increased starch granules fragility during cooking.
31899241	4	11	theme	flour	469:473	arg1	properties					443:452	pasting properties	435:452	pasting properties	435:452	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	4	11	theme	flour	469:473	arg1	composition					410:420	Chemical composition	401:420	Chemical composition	401:420	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	4	12	theme	Chemical	401:408	arg1	composition					410:420	Chemical composition	401:420	Chemical composition	401:420	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	12	13	theme	progenies	1501:1509	arg1	identification					1483:1496	the identification	1479:1496	the identification of progenies with particular properties for production of functional maize based-foods	1479:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	9	14	theme	lower	992:996	arg1	content					1006:1012	the lower amylose content	988:1012	the lower amylose content in relation to non-purple	988:1038	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	4	15	theme	grain	463:467	arg1	flour					469:473	whole grain flour	457:473	whole grain flour	457:473	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	2	16	theme	environmental	249:261	arg1	effects					263:269	the environmental effects	245:269	the environmental effects on composition and flour properties	245:305	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	5	17	theme	Non-purple	492:501	arg1	controls					526:533	controls	526:533	controls	526:533	Non-purple genotypes were used as controls.
31899241	5	17	theme	Non-purple	492:501	arg1	genotypes					503:511	Non-purple genotypes	492:511	Non-purple genotypes	492:511	Non-purple genotypes were used as controls.
31899241	2	18	from	flour	191:195	arg1	Argentina					218:226	Argentina	218:226	Argentina	218:226	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	9	19	from	content	1006:1012	arg1	relation					1017:1024	relation	1017:1024	relation to non-purple	1017:1038	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	6	20	theme	significant	577:587	arg1	differences					589:599	significant differences	577:599	significant differences with controls	577:613	Composition of purple maize did not show significant differences with controls, but amylose content was significantly lower.
31899241	12	21	theme	particular	1516:1525	arg1	properties					1527:1536	particular properties	1516:1536	particular properties for production of functional maize based-foods	1516:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	2	22	from	Argentina	218:226	arg1	flour					191:195	flour	191:195	flour of purple maize from Argentina	191:226	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	2	22	from	Argentina	218:226	arg1	maize					207:211	purple maize	200:211	purple maize from Argentina	200:226	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	0	23	theme	functional	6:15	arg1	properties					17:26	Flour functional properties	0:26	Flour functional properties of purple maize (Zea mays L.) from Argentina.	0:72	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	7	24	from	variability	666:676	arg1	properties					701:710	pasting and thermal properties	681:710	pasting and thermal properties of flour	681:719	High variability in pasting and thermal properties of flour was observed between genotypes.
31899241	8	25	dep	breakdown	800:808	arg1	P < 0.05					821:828	P < 0.05	821:828	P < 0.05	821:828	Anthocyanin content positively correlated with breakdown (r = 0.37, P < 0.05), indicating that anthocyanins increased starch granules fragility during cooking.
31899241	8	25	dep	breakdown	800:808	arg1	r = 0.37					811:818	r = 0.37	811:818	r = 0.37	811:818	Anthocyanin content positively correlated with breakdown (r = 0.37, P < 0.05), indicating that anthocyanins increased starch granules fragility during cooking.
31899241	0	26	theme	Flour	0:4	arg1	properties					17:26	Flour functional properties	0:26	Flour functional properties of purple maize (Zea mays L.) from Argentina.	0:72	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	12	27	theme	maize	1567:1571	arg1	based-foods					1573:1583	functional maize based-foods	1556:1583	functional maize based-foods	1556:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	2	28	theme	flour	290:294	arg1	properties					296:305	flour properties	290:305	flour properties	290:305	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	7	29	theme	flour	715:719	arg1	properties					701:710	pasting and thermal properties	681:710	pasting and thermal properties of flour	681:719	High variability in pasting and thermal properties of flour was observed between genotypes.
31899241	10	30	dep	anthocyanins	1146:1157	arg1	P < 0.05					1171:1178	P < 0.05	1171:1178	P < 0.05	1171:1178	The amylopectin retrogradation enthalpy negatively correlated with polyphenols (r = -0.35, P < 0.05) and anthocyanins (r = -0.40, P < 0.05), probably due to interactions formed after starch gelatinization.
31899241	11	31	theme	Flour	1247:1251	arg1	parameters					1267:1276	Flour functionality parameters	1247:1276	Flour functionality parameters	1247:1276	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	11	32	theme	genotype	1302:1309	arg1	effects					1321:1327	lower effects	1315:1327	lower effects of environment and genotype × environment interaction	1315:1381	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	11	32	theme	genotype	1302:1309	arg1	effect					1292:1297	higher effect	1285:1297	higher effect of genotype	1285:1309	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	2	33	theme	viscosity	167:175	arg1	properties					177:186	thermal and viscosity properties	155:186	properties	177:186	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	11	34	theme	functionality	1253:1265	arg1	parameters					1267:1276	Flour functionality parameters	1247:1276	Flour functionality parameters	1247:1276	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	0	35	theme	maize	38:42	arg1	properties					17:26	Flour functional properties	0:26	Flour functional properties of purple maize (Zea mays L.) from Argentina.	0:72	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	11	36	theme	higher	1285:1290	arg1	effect					1292:1297	higher effect	1285:1297	higher effect of genotype	1285:1309	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	4	37	theme	pasting	435:441	arg1	properties					443:452	pasting properties	435:452	pasting properties	435:452	Chemical composition, thermal and pasting properties of whole grain flour were determined.
31899241	5	38	used	used	518:521	arg2	genotypes					503:511	Non-purple genotypes	492:511	Non-purple genotypes	492:511	Non-purple genotypes were used as controls.
31899241	5	38	used	used	518:521	arg2	controls					526:533	controls	526:533	controls	526:533	Non-purple genotypes were used as controls.
31899241	0	39	theme	purple	31:36	arg1	maize					38:42	purple maize	31:42	purple maize (Zea mays L.)	31:56	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	0	39	theme	purple	31:36	arg1	L.					54:55	Zea mays L.	45:55	Zea mays L.	45:55	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	8	40	theme	Anthocyanin	753:763	arg1	content					765:771	Anthocyanin content	753:771	Anthocyanin content	753:771	Anthocyanin content positively correlated with breakdown (r = 0.37, P < 0.05), indicating that anthocyanins increased starch granules fragility during cooking.
31899241	2	41	theme	thermal	155:161	arg1	properties					177:186	thermal and viscosity properties	155:186	properties	177:186	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	12	42	theme	flour	1442:1446	arg1	behavior					1448:1455	different flour behavior	1432:1455	different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods	1432:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	9	43	theme	amylose	998:1004	arg1	content					1006:1012	the lower amylose content	988:1012	the lower amylose content in relation to non-purple	988:1038	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	0	44	theme	Zea	45:47	arg1	maize					38:42	purple maize	31:42	purple maize (Zea mays L.)	31:56	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	0	44	theme	Zea	45:47	arg1	L.					54:55	Zea mays L.	45:55	Zea mays L.	45:55	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	9	45	theme	higher	917:922	arg1	coincident					972:981	coincident	972:981	coincident	972:981	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	9	45	theme	higher	917:922	arg1	enthalpy					939:946	The higher gelatinization enthalpy	913:946	The higher gelatinization enthalpy of purple genotypes	913:966	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	6	46	theme	purple	551:556	arg1	maize					558:562	purple maize	551:562	purple maize	551:562	Composition of purple maize did not show significant differences with controls, but amylose content was significantly lower.
31899241	2	47	theme	maize	207:211	arg1	flour					191:195	flour	191:195	flour of purple maize from Argentina	191:226	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	12	48	theme	functional	1556:1565	arg1	based-foods					1573:1583	functional maize based-foods	1556:1583	functional maize based-foods	1556:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	1	49	theme	environmental	87:99	arg1	conditions					109:118	environmental growing conditions	87:118	environmental growing conditions	87:118	Influence of environmental growing conditions.
31899241	9	50	theme	gelatinization	924:937	arg1	coincident					972:981	coincident	972:981	coincident	972:981	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	9	50	theme	gelatinization	924:937	arg1	enthalpy					939:946	The higher gelatinization enthalpy	913:946	The higher gelatinization enthalpy of purple genotypes	913:966	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	7	51	theme	pasting	681:687	arg1	properties					701:710	pasting and thermal properties	681:710	pasting and thermal properties of flour	681:719	High variability in pasting and thermal properties of flour was observed between genotypes.
31899241	2	52	theme	purple	200:205	arg1	maize					207:211	purple maize	200:211	purple maize from Argentina	200:226	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	7	53	theme	thermal	693:699	arg1	properties					701:710	pasting and thermal properties	681:710	pasting and thermal properties of flour	681:719	High variability in pasting and thermal properties of flour was observed between genotypes.
31899241	1	54	theme	growing	101:107	arg1	conditions					109:118	environmental growing conditions	87:118	environmental growing conditions	87:118	Influence of environmental growing conditions.
31899241	2	55	theme	flour	191:195	arg1	properties					177:186	thermal and viscosity properties	155:186	properties	177:186	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	10	56	theme	amylopectin	1045:1055	arg1	enthalpy					1072:1079	The amylopectin retrogradation enthalpy	1041:1079	The amylopectin retrogradation enthalpy	1041:1079	The amylopectin retrogradation enthalpy negatively correlated with polyphenols (r = -0.35, P < 0.05) and anthocyanins (r = -0.40, P < 0.05), probably due to interactions formed after starch gelatinization.
31899241	1	57	theme	conditions	109:118	arg1	Influence					74:82	Influence	74:82	Influence of environmental growing conditions	74:118	Influence of environmental growing conditions.
31899241	10	58	dep	polyphenols	1108:1118	arg1	P < 0.05					1132:1139	P < 0.05	1132:1139	P < 0.05	1132:1139	The amylopectin retrogradation enthalpy negatively correlated with polyphenols (r = -0.35, P < 0.05) and anthocyanins (r = -0.40, P < 0.05), probably due to interactions formed after starch gelatinization.
31899241	11	59	theme	lower	1315:1319	arg1	effects					1321:1327	lower effects	1315:1327	lower effects of environment and genotype × environment interaction	1315:1381	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	9	60	with	coincident	972:981	arg1	content					1006:1012	the lower amylose content	988:1012	the lower amylose content in relation to non-purple	988:1038	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	12	61	with	progenies	1501:1509	arg1	properties					1527:1536	particular properties	1516:1536	particular properties for production of functional maize based-foods	1516:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	6	62	theme	maize	558:562	arg1	Composition					536:546	Composition	536:546	Composition of purple maize	536:562	Composition of purple maize did not show significant differences with controls, but amylose content was significantly lower.
31899241	0	63	from	Argentina	63:71	arg1	properties					17:26	Flour functional properties	0:26	Flour functional properties of purple maize (Zea mays L.) from Argentina.	0:72	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	12	64	theme	different	1432:1440	arg1	behavior					1448:1455	different flour behavior	1432:1455	different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods	1432:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	2	65	from	effects	263:269	arg1	composition					274:284	composition	274:284	composition	274:284	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	2	65	from	effects	263:269	arg1	properties					296:305	flour properties	290:305	flour properties	290:305	The objective was to characterize thermal and viscosity properties of flour of purple maize from Argentina, and to evaluate the environmental effects on composition and flour properties.
31899241	10	66	theme	starch	1224:1229	arg1	gelatinization					1231:1244	starch gelatinization	1224:1244	starch gelatinization	1224:1244	The amylopectin retrogradation enthalpy negatively correlated with polyphenols (r = -0.35, P < 0.05) and anthocyanins (r = -0.40, P < 0.05), probably due to interactions formed after starch gelatinization.
31899241	0	67	theme	mays	49:52	arg1	maize					38:42	purple maize	31:42	purple maize (Zea mays L.)	31:56	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	0	67	theme	mays	49:52	arg1	L.					54:55	Zea mays L.	45:55	Zea mays L.	45:55	Flour functional properties of purple maize (Zea mays L.) from Argentina.
31899241	12	68	theme	based-foods	1573:1583	arg1	production					1542:1551	production	1542:1551	production of functional maize based-foods	1542:1583	The variability found among genotypes indicates different flour behavior that would facilitate the identification of progenies with particular properties for production of functional maize based-foods.
31899241	9	69	theme	genotypes	958:966	arg1	coincident					972:981	coincident	972:981	coincident	972:981	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	9	69	theme	genotypes	958:966	arg1	enthalpy					939:946	The higher gelatinization enthalpy	913:946	The higher gelatinization enthalpy of purple genotypes	913:966	The higher gelatinization enthalpy of purple genotypes was coincident with the lower amylose content in relation to non-purple.
31899241	11	70	dep	environment	1332:1342	arg1	interaction					1371:1381	interaction	1371:1381	interaction	1371:1381	Flour functionality parameters showed higher effect of genotype and lower effects of environment and genotype × environment interaction.
31899241	8	71	theme	starch	871:876	arg1	granules					878:885	starch granules	871:885	starch granules fragility	871:895	Anthocyanin content positively correlated with breakdown (r = 0.37, P < 0.05), indicating that anthocyanins increased starch granules fragility during cooking.
31229868	0	0	theme	magnetic	85:92	arg1	composites					103:112	ionic liquids modified magnetic alginate composites	62:112	ionic liquids modified magnetic alginate composites	62:112	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	7	1	theme	constant	1096:1103	arg1	lower					1137:1141	lower	1137:1141	lower	1137:1141	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	7	1	theme	constant	1096:1103	arg1	Km					1105:1106	the kinetic constant Km	1084:1106	the kinetic constant Km of PPL-IL-MSA	1084:1120	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	2	2	dep	infrared	304:311	arg1	FTIR					314:317	FTIR	314:317	FTIR	314:317	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	0	3	theme	modified	76:83	arg1	composites					103:112	ionic liquids modified magnetic alginate composites	62:112	ionic liquids modified magnetic alginate composites	62:112	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	1	4	theme	magnetic	156:163	arg1	nanoparticles					174:186	magnetic alginate nanoparticles	156:186	magnetic alginate nanoparticles	156:186	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	8	5	theme	PPL-IL-MSA	1352:1361	arg1	improvement					1337:1347	the performance improvement	1321:1347	the performance improvement of PPL-IL-MSA	1321:1361	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	8	6	theme	secondary	1253:1261	arg1	structure					1263:1271	the secondary structure	1249:1271	the secondary structure of enzymes	1249:1282	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	2	7	theme	infrared	304:311	arg1	spectroscopy					320:331	infrared (FTIR) spectroscopy	304:331	infrared (FTIR) spectroscopy	304:331	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	0	8	theme	alginate	94:101	arg1	composites					103:112	ionic liquids modified magnetic alginate composites	62:112	ionic liquids modified magnetic alginate composites	62:112	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	4	9	theme	free	809:812	arg1	lipase					814:819	free lipase	809:819	free lipase	809:819	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	2	10	theme	angle	487:491	arg1	measurements					499:510	water contact angle (WCA) measurements	473:510	water contact angle (WCA) measurements	473:510	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	7	11	theme	free	1156:1159	arg1	21.2 mg/mL					1169:1178	21.2 mg/mL	1169:1178	21.2 mg/mL	1169:1178	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	7	11	theme	free	1156:1159	arg1	lipase					1161:1166	free lipase	1156:1166	free lipase (21.2 mg/mL)	1156:1179	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	6	12	theme	residual	992:999	arg1	activity					1001:1008	the residual activity	988:1008	the residual activity of PPL-IL-MSA	988:1022	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	6	12	theme	residual	992:999	arg1	higher					1034:1039	higher	1034:1039	higher	1034:1039	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	5	13	theme	PPL-IL-MSA	906:915	arg1	stability					846:854	The thermal stability	834:854	The thermal stability	834:854	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	5	13	theme	PPL-IL-MSA	906:915	arg1	stability					870:878	denaturation stability	857:878	denaturation stability	857:878	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	5	13	theme	PPL-IL-MSA	906:915	arg1	higher					927:932	higher	927:932	higher	927:932	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	5	13	theme	PPL-IL-MSA	906:915	arg1	stability					893:901	reusing stability	885:901	reusing stability	885:901	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	9	14	from	studies	1481:1487	arg1	field					1497:1501	this field	1492:1501	this field	1492:1501	This work involving the development of a new supports for enzyme immobilization may serve as a reference for further studies in this field.
31229868	1	15	theme	alginate	165:172	arg1	nanoparticles					174:186	magnetic alginate nanoparticles	156:186	magnetic alginate nanoparticles	156:186	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	2	16	theme	contact	479:485	arg1	angle					487:491	water contact angle	473:491	water contact angle (WCA) measurements	473:510	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	16	theme	contact	479:485	arg1	WCA					494:496	WCA	494:496	WCA	494:496	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	4	17	theme	pH	715:716	arg1	range					746:750	the pH and temperature application range	711:750	the pH and temperature application range of PPL-IL-MSA	711:764	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	17	theme	pH	715:716	arg1	broader					788:794	broader	788:794	broader	788:794	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	2	18	dep	novel	246:250	arg1	supports					252:259	supports	252:259	supports	252:259	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	0	19	theme	catalytic	9:17	arg1	performance					19:29	Enhanced catalytic performance	0:29	Enhanced catalytic performance of lipase	0:39	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	0	20	theme	liquids	68:74	arg1	composites					103:112	ionic liquids modified magnetic alginate composites	62:112	ionic liquids modified magnetic alginate composites	62:112	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	2	21	theme	sample	380:385	arg1	magnetometry					387:398	vibrating sample magnetometry	370:398	vibrating sample magnetometry (VSM)	370:404	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	21	theme	sample	380:385	arg1	VSM					401:403	VSM	401:403	VSM	401:403	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	0	22	theme	Enhanced	0:7	arg1	performance					19:29	Enhanced catalytic performance	0:29	Enhanced catalytic performance of lipase	0:39	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	3	23	theme	higher	583:588	arg1	activity					564:571	high activity	559:571	high activity	559:571	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	2	24	theme	vibrating	370:378	arg1	magnetometry					387:398	vibrating sample magnetometry	370:398	vibrating sample magnetometry (VSM)	370:404	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	24	theme	vibrating	370:378	arg1	VSM					401:403	VSM	401:403	VSM	401:403	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	3	25	theme	immobilized	517:527	arg1	lipase					529:534	The immobilized lipase	513:534	The immobilized lipase (PPL-IL-MSA)	513:547	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	3	25	theme	immobilized	517:527	arg1	PPL-IL-MSA					537:546	PPL-IL-MSA	537:546	PPL-IL-MSA	537:546	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	5	26	theme	reusing	885:891	arg1	stability					893:901	reusing stability	885:901	reusing stability	885:901	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	8	27	used	used	1235:1238	arg2	CD					1227:1228	CD	1227:1228	CD	1227:1228	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	8	27	used	used	1235:1238	arg2	dichroism					1216:1224	Circular dichroism	1207:1224	Circular dichroism (CD)	1207:1229	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	2	28	theme	magnetic	342:349	arg1	1HNMR					362:366	1HNMR	362:366	1HNMR	362:366	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	28	theme	magnetic	342:349	arg1	resonance					351:359	nuclear magnetic resonance	334:359	nuclear magnetic resonance (1HNMR)	334:367	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	3	29	theme	free	612:615	arg1	lipase					617:622	free lipase	612:622	free lipase	612:622	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	3	30	theme	high	559:562	arg1	activity					564:571	high activity	559:571	high activity	559:571	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	6	31	theme	reuse	981:985	arg1	times					972:976	10 times	969:976	10 times of reuse	969:985	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	0	32	theme	lipase	34:39	arg1	performance					19:29	Enhanced catalytic performance	0:29	Enhanced catalytic performance of lipase	0:39	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	4	33	theme	PPL-IL-MSA	755:764	arg1	range					746:750	the pH and temperature application range	711:750	the pH and temperature application range of PPL-IL-MSA	711:764	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	33	theme	PPL-IL-MSA	755:764	arg1	broader					788:794	broader	788:794	broader	788:794	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	2	34	theme	electron	443:450	arg1	TEM					464:466	TEM	464:466	TEM	464:466	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	34	theme	electron	443:450	arg1	microscopy					452:461	transmission electron microscopy	430:461	transmission electron microscopy (TEM)	430:467	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	8	35	theme	Circular	1207:1214	arg1	dichroism					1216:1224	Circular dichroism	1207:1224	Circular dichroism (CD)	1207:1229	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	8	35	theme	Circular	1207:1214	arg1	CD					1227:1228	CD	1227:1228	CD	1227:1228	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	7	36	theme	PPL-IL-MSA	1111:1120	arg1	lower					1137:1141	lower	1137:1141	lower	1137:1141	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	7	36	theme	PPL-IL-MSA	1111:1120	arg1	Km					1105:1106	the kinetic constant Km	1084:1106	the kinetic constant Km of PPL-IL-MSA	1084:1120	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	8	37	theme	improvement	1337:1347	arg1	mechanism					1308:1316	the mechanism	1304:1316	the mechanism of the performance improvement of PPL-IL-MSA	1304:1361	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	5	38	theme	thermal	838:844	arg1	stability					846:854	The thermal stability	834:854	The thermal stability	834:854	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	5	38	theme	thermal	838:844	arg1	higher					927:932	higher	927:932	higher	927:932	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	2	39	theme	transmission	430:441	arg1	TEM					464:466	TEM	464:466	TEM	464:466	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	39	theme	transmission	430:441	arg1	microscopy					452:461	transmission electron microscopy	430:461	transmission electron microscopy (TEM)	430:467	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	7	40	theme	kinetic	1088:1094	arg1	lower					1137:1141	lower	1137:1141	lower	1137:1141	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	7	40	theme	kinetic	1088:1094	arg1	Km					1105:1106	the kinetic constant Km	1084:1106	the kinetic constant Km of PPL-IL-MSA	1084:1120	Furthermore, the kinetic constant Km of PPL-IL-MSA was 13.7 mg/mL lower than that of free lipase (21.2 mg/mL) and PPL-MSA (18.4 mg/mL).
31229868	1	41	theme	ionic	127:131	arg1	liquids					133:139	ionic liquids	127:139	ionic liquids (ILs) modified magnetic alginate nanoparticles	127:186	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	1	41	theme	ionic	127:131	arg1	ILs					142:144	ILs	142:144	ILs	142:144	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	8	42	theme	enzymes	1276:1282	arg1	structure					1263:1271	the secondary structure	1249:1271	the secondary structure of enzymes	1249:1282	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	3	43	theme	higher	638:643	arg1	activity					564:571	high activity	559:571	high activity	559:571	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	1	44	used	used	192:195	arg2	We					115:116	We	115:116	We	115:116	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	5	45	theme	other	948:952	arg1	samples					954:960	other samples	948:960	other samples	948:960	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	2	46	theme	nuclear	334:340	arg1	1HNMR					362:366	1HNMR	362:366	1HNMR	362:366	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	46	theme	nuclear	334:340	arg1	resonance					351:359	nuclear magnetic resonance	334:359	nuclear magnetic resonance (1HNMR)	334:367	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	9	47	theme	enzyme	1422:1427	arg1	reference					1459:1467	a reference	1457:1467	a reference for further studies in this field	1457:1501	This work involving the development of a new supports for enzyme immobilization may serve as a reference for further studies in this field.
31229868	9	47	theme	enzyme	1422:1427	arg1	immobilization					1429:1442	enzyme immobilization	1422:1442	enzyme immobilization	1422:1442	This work involving the development of a new supports for enzyme immobilization may serve as a reference for further studies in this field.
31229868	1	48	theme	lipase	219:224	arg1	immobilization					226:239	lipase immobilization	219:239	lipase immobilization	219:239	We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization.
31229868	9	49	theme	new	1405:1407	arg1	development					1388:1398	the development	1384:1398	the development of a new	1384:1407	This work involving the development of a new supports for enzyme immobilization may serve as a reference for further studies in this field.
31229868	8	50	theme	performance	1325:1335	arg1	improvement					1337:1347	the performance improvement	1321:1347	the performance improvement of PPL-IL-MSA	1321:1361	Circular dichroism (CD) was used to study the secondary structure of enzymes in order to explain the mechanism of the performance improvement of PPL-IL-MSA.
31229868	4	51	theme	temperature	722:732	arg1	range					746:750	the pH and temperature application range	711:750	the pH and temperature application range of PPL-IL-MSA	711:764	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	51	theme	temperature	722:732	arg1	broader					788:794	broader	788:794	broader	788:794	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	5	52	theme	denaturation	857:868	arg1	stability					870:878	denaturation stability	857:878	denaturation stability	857:878	The thermal stability, denaturation stability, and reusing stability of PPL-IL-MSA were also higher than those of other samples.
31229868	4	53	theme	application	734:744	arg1	range					746:750	the pH and temperature application range	711:750	the pH and temperature application range of PPL-IL-MSA	711:764	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	53	theme	application	734:744	arg1	broader					788:794	broader	788:794	broader	788:794	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	2	54	theme	water	473:477	arg1	angle					487:491	water contact angle	473:491	water contact angle (WCA) measurements	473:510	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	2	54	theme	water	473:477	arg1	WCA					494:496	WCA	494:496	WCA	494:496	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	9	55	theme	further	1473:1479	arg1	studies					1481:1487	further studies	1473:1487	further studies in this field	1473:1501	This work involving the development of a new supports for enzyme immobilization may serve as a reference for further studies in this field.
31229868	2	56	dep	Fourier	286:292	arg1	transform					294:302	transform	294:302	transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements	294:510	The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (1HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements.
31229868	0	57	theme	ionic	62:66	arg1	composites					103:112	ionic liquids modified magnetic alginate composites	62:112	ionic liquids modified magnetic alginate composites	62:112	Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.
31229868	3	58	theme	immobilized	657:667	arg1	lipase					669:674	immobilized lipase	657:674	immobilized lipase without IL (PPL-MSA)	657:695	The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.1-fold higher than that compared to free lipase and 1.59-fold higher compared to immobilized lipase without IL (PPL-MSA).
31229868	6	59	dep	higher	1034:1039	arg1	%					1067:1067	84.4%	1063:1067	84.4%	1063:1067	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	6	60	theme	PPL-IL-MSA	1013:1022	arg1	activity					1001:1008	the residual activity	988:1008	the residual activity of PPL-IL-MSA	988:1022	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	6	60	theme	PPL-IL-MSA	1013:1022	arg1	higher					1034:1039	higher	1034:1039	higher	1034:1039	After 10 times of reuse, the residual activity of PPL-IL-MSA was 89.7% higher than that of PPL-MSA (84.4%).
31229868	4	61	located	found	776:780	arg1	addition					701:708	addition	701:708	addition	701:708	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	61	located	found	776:780	arg2	broader					788:794	broader	788:794	broader	788:794	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31229868	4	61	located	found	776:780	arg2	range					746:750	the pH and temperature application range	711:750	the pH and temperature application range of PPL-IL-MSA	711:764	In addition, the pH and temperature application range of PPL-IL-MSA were both found to be broader than that of free lipase and PPL-MSA.
31028908	6	0	dep	hydrogels	1308:1316	arg1	%					1328:1328	6.4 ± 7.2%	1319:1328	6.4 ± 7.2% average error	1319:1342	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	9	1	theme	molded	1698:1703	arg1	templates					1713:1721	injection molded PVOH-Ca templates	1688:1721	injection molded PVOH-Ca templates	1688:1721	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	13	2	link	cross-linked	2616:2627	arg1	hydrogels					2638:2646	ionically cross-linked alginate hydrogels	2606:2646	ionically cross-linked alginate hydrogels	2606:2646	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	15	3	theme	architecture	2998:3009	arg1	customization					2963:2975	scalable 3D customization	2951:2975	scalable 3D customization of hydrogel internal architecture	2951:3009	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	2	4	theme	solvent	356:362	arg1	casting					364:370	solvent casting	356:370	solvent casting	356:370	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	10	5	theme	tissue	2035:2040	arg1	applications					2054:2065	tissue engineering applications	2035:2065	tissue engineering applications	2035:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	15	6	theme	3D	2960:2961	arg1	customization					2963:2975	scalable 3D customization	2951:2975	scalable 3D customization of hydrogel internal architecture	2951:3009	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	1	7	theme	control	259:265	arg1	morphology					274:283	control tissue morphology	259:283	control tissue morphology	259:283	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	7	8	theme	engineering	1466:1476	arg1	scalability					1478:1488	engineering scalability	1466:1488	engineering scalability	1466:1488	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	10	9	dep	STATEMENT	1915:1923	arg1	molding					1954:1960	Sacrificial molding	1942:1960	Sacrificial molding of hydrogel monoliths	1942:1982	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	10	9	dep	STATEMENT	1915:1923	arg1	technique					1999:2007	a versatile technique	1987:2007	a versatile technique for creating 3D molds for tissue engineering applications	1987:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	4	10	link	cross-linked	901:912	arg1	hydrogels					923:931	ionically cross-linked alginate hydrogels	891:931	ionically cross-linked alginate hydrogels	891:931	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	6	11	theme	alginate	1299:1306	arg1	hydrogels					1308:1316	alginate hydrogels	1299:1316	alginate hydrogels (6.4 ± 7.2% average error)	1299:1343	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	1	12	theme	sacrificial	167:177	arg1	molding					179:185	sacrificial molding	167:185	sacrificial molding of hydrogel monoliths	167:207	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	1	12	theme	sacrificial	167:177	arg1	technique					224:232	a versatile technique	212:232	a versatile technique for creating 3D molds to control tissue morphology	212:283	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	4	13	theme	bulk	865:868	arg1	hydrogels					877:885	bulk curing hydrogels	865:885	bulk curing hydrogels	865:885	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	14	theme	poly	684:687	arg1	PVOH-Ca					728:734	PVOH-Ca	728:734	PVOH-Ca	728:734	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	14	theme	poly	684:687	arg1	templates					717:725	poly(vinyl alcohol)-calcium salt templates	684:725	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process,	684:810	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	6	15	theme	geometries	1281:1290	arg1	casting					1251:1257	precise casting	1243:1257	precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error)	1243:1343	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	11	16	theme	mold	2160:2163	arg1	geometries					2174:2183	mold internal geometries	2160:2183	mold internal geometries within rapidly polymerizing, bulk curing hydrogels	2160:2234	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	9	17	theme	PVOH-Ca	1705:1711	arg1	templates					1713:1721	injection molded PVOH-Ca templates	1688:1721	injection molded PVOH-Ca templates	1688:1721	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	9	18	theme	molding	1772:1778	arg1	customization					1734:1746	customization	1734:1746	customization	1734:1746	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	0	19	theme	hydrogel	99:106	arg1	architecture					117:128	3D hydrogel internal architecture	96:128	3D hydrogel internal architecture	96:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	8	20	dep	microscale	1635:1644	arg1	biomimetic					1647:1656	biomimetic	1647:1656	biomimetic	1647:1656	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	2	21	theme	sacrificial	295:305	arg1	templates					307:315	Previous sacrificial templates	286:315	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing	286:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	1	22	theme	monoliths	199:207	arg1	molding					179:185	sacrificial molding	167:185	sacrificial molding of hydrogel monoliths	167:207	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	1	22	theme	monoliths	199:207	arg1	technique					224:232	a versatile technique	212:232	a versatile technique for creating 3D molds to control tissue morphology	212:283	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	13	23	theme	vinyl	2399:2403	arg1	poly					2394:2397	poly	2394:2397	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process,	2394:2525	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	23	theme	vinyl	2399:2403	arg1	alcohol					2405:2411	vinyl alcohol	2399:2411	vinyl alcohol	2399:2411	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	11	24	theme	internal	2165:2172	arg1	geometries					2174:2183	mold internal geometries	2160:2183	mold internal geometries within rapidly polymerizing, bulk curing hydrogels	2160:2234	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	7	25	theme	alginate	1419:1426	arg1	hydrogels					1428:1436	alginate hydrogels	1419:1436	alginate hydrogels	1419:1436	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	0	26	theme	architecture	117:128	arg1	customization					79:91	precise customization	71:91	precise customization of 3D hydrogel internal architecture	71:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	9	27	dep	molded	1698:1703	arg1	injection					1688:1696	injection	1688:1696	injection	1688:1696	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	4	28	theme	alginate	914:921	arg1	hydrogels					923:931	ionically cross-linked alginate hydrogels	891:931	ionically cross-linked alginate hydrogels	891:931	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	29	theme	salt	712:715	arg1	PVOH-Ca					728:734	PVOH-Ca	728:734	PVOH-Ca	728:734	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	29	theme	salt	712:715	arg1	templates					717:725	poly(vinyl alcohol)-calcium salt templates	684:725	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process,	684:810	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	8	30	used	used	1517:1520	arg2	platform					1505:1512	the platform	1501:1512	the platform	1501:1512	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	5	31	theme	salt	942:945	arg1	factor					990:995	a critical factor	979:995	a critical factor	979:995	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	5	31	theme	salt	942:945	arg1	solubility					947:956	Calcium salt solubility	934:956	Calcium salt solubility	934:956	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	11	32	theme	polymerizing	2200:2211	arg1	hydrogels					2226:2234	rapidly polymerizing, bulk curing hydrogels	2192:2234	rapidly polymerizing, bulk curing hydrogels	2192:2234	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	6	33	theme	precise	1243:1249	arg1	casting					1251:1257	precise casting	1243:1257	precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error)	1243:1343	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	7	34	theme	3D	1392:1393	arg1	networks					1403:1410	mold 3D channel networks	1387:1410	mold 3D channel networks within alginate hydrogels	1387:1436	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	1	35	from	technique	224:232	arg1	applications					153:164	tissue engineering applications	134:164	tissue engineering applications	134:164	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	9	36	theme	3D	1811:1812	arg1	hydrogels					1814:1822	3D hydrogels	1811:1822	3D hydrogels	1811:1822	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	4	37	theme	vinyl	689:693	arg1	alcohol					695:701	vinyl alcohol	689:701	vinyl alcohol	689:701	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	37	theme	vinyl	689:693	arg1	poly					684:687	poly	684:687	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process,	684:810	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	0	38	theme	-calcium	43:50	arg1	templates					57:65	poly(vinyl alcohol)-calcium salt templates	24:65	poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture	24:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	1	39	theme	tissue	134:139	arg1	applications					153:164	tissue engineering applications	134:164	tissue engineering applications	134:164	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	14	40	theme	hydrogels	2740:2748	arg1	molding					2720:2726	precise sacrificial molding	2700:2726	precise sacrificial molding of alginate hydrogels	2700:2748	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	15	41	theme	internal	2989:2996	arg1	architecture					2998:3009	hydrogel internal architecture	2980:3009	hydrogel internal architecture	2980:3009	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	12	42	dep	alginate	2361:2368	arg1	e.g.					2356:2359	e.g.	2356:2359	e.g.	2356:2359	However, they display poor performance in molding geometry within diffusion limited, ionically cross-linked hydrogels, e.g. alginate.
31028908	9	43	theme	internal	1837:1844	arg1	architecture					1857:1868	complex internal microscale architecture	1829:1868	complex internal microscale architecture for diverse tissue engineering applications	1829:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	0	44	theme	precise	71:77	arg1	customization					79:91	precise customization	71:91	precise customization of 3D hydrogel internal architecture	71:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	5	45	theme	template	1119:1126	arg1	dissolution					1128:1138	template dissolution	1119:1138	template dissolution	1119:1138	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	8	46	link	-derived	1595:1602	arg1	organoids					1620:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids	1563:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	14	47	theme	sacrificial	2708:2718	arg1	molding					2720:2726	precise sacrificial molding	2700:2726	precise sacrificial molding of alginate hydrogels	2700:2748	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	8	48	theme	embryonic	1569:1577	arg1	hESC					1590:1593	hESC	1590:1593	hESC	1590:1593	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	8	48	theme	embryonic	1569:1577	arg1	cell					1584:1587	human embryonic stem cell	1563:1587	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	13	49	theme	bulk	2580:2583	arg1	hydrogels					2592:2600	bulk curing hydrogels	2580:2600	bulk curing hydrogels	2580:2600	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	7	50	theme	templates	1374:1382	arg1	Assembly					1346:1353	Assembly	1346:1353	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels	1346:1436	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	9	51	theme	diverse	1874:1880	arg1	applications					1901:1912	diverse tissue engineering applications	1874:1912	diverse tissue engineering applications	1874:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	8	52	theme	cell	1584:1587	arg1	organoids					1620:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids	1563:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	15	53	theme	hPSC-derived	2847:2858	arg1	organoids					2876:2884	hPSC-derived neuroepithelial organoids	2847:2884	hPSC-derived neuroepithelial organoids	2847:2884	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	9	54	theme	hydrogel	1751:1758	arg1	molding					1772:1778	hydrogel sacrificial molding	1751:1778	hydrogel sacrificial molding	1751:1778	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	1	55	theme	tissue	267:272	arg1	morphology					274:283	control tissue morphology	259:283	control tissue morphology	259:283	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	7	56	theme	modular	1358:1364	arg1	templates					1374:1382	modular PVOH-Ca templates	1358:1382	modular PVOH-Ca templates	1358:1382	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	9	57	theme	engineering	1889:1899	arg1	applications					1901:1912	diverse tissue engineering applications	1874:1912	diverse tissue engineering applications	1874:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	14	58	from	templates	2682:2690	arg1	release					2657:2663	Calcium release	2649:2663	Calcium release from the PVOH-Ca templates	2649:2690	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	2	59	theme	mold	435:438	arg1	geometries					449:458	mold internal geometries	435:458	mold internal geometries within rapidly polymerizing, bulk curing hydrogels	435:509	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	15	60	theme	template	2907:2914	arg1	designs					2916:2922	modular PVOH-Ca template designs	2891:2922	modular PVOH-Ca template designs	2891:2922	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	14	61	theme	precise	2700:2706	arg1	molding					2720:2726	precise sacrificial molding	2700:2726	precise sacrificial molding of alginate hydrogels	2700:2748	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	13	62	theme	internal	2548:2555	arg1	geometries					2557:2566	internal geometries	2548:2566	internal geometries	2548:2566	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	8	63	theme	-derived	1595:1602	arg1	organoids					1620:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids	1563:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	12	64	theme	limited	2313:2319	arg1	hydrogels					2345:2353	diffusion limited, ionically cross-linked hydrogels	2303:2353	diffusion limited, ionically cross-linked hydrogels	2303:2353	However, they display poor performance in molding geometry within diffusion limited, ionically cross-linked hydrogels, e.g. alginate.
31028908	15	65	theme	modular	2891:2897	arg1	designs					2916:2922	modular PVOH-Ca template designs	2891:2922	modular PVOH-Ca template designs	2891:2922	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	9	66	used	used	1794:1797	arg2	customization					1734:1746	customization	1734:1746	customization	1734:1746	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	13	67	theme	mass-production	2502:2516	arg1	process					2518:2524	an unparalleled mass-production process	2486:2524	an unparalleled mass-production process	2486:2524	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	67	theme	mass-production	2502:2516	arg1	molding					2477:2483	micro-injection molding	2461:2483	micro-injection molding	2461:2483	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	11	68	dep	curing	2219:2224	arg1	bulk					2214:2217	bulk	2214:2217	bulk	2214:2217	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	15	69	theme	neuroepithelial	2860:2874	arg1	organoids					2876:2884	hPSC-derived neuroepithelial organoids	2847:2884	hPSC-derived neuroepithelial organoids	2847:2884	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	4	70	theme	internal	833:840	arg1	geometries					842:851	internal geometries	833:851	internal geometries	833:851	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	2	71	dep	hydrogels	501:509	arg1	curing					494:499	bulk curing	489:499	bulk curing	489:499	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	6	72	theme	tomography	1167:1176	arg1	analysis					1183:1190	Metrological and computed tomography (CT) analysis	1141:1190	Metrological and computed tomography (CT) analysis	1141:1190	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	6	73	theme	calcium	1219:1225	arg1	release					1227:1233	the template's calcium release	1204:1233	the template's calcium release	1204:1233	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	4	74	theme	mass-production	787:801	arg1	process					803:809	a parallel mass-production process	776:809	a parallel mass-production process	776:809	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	74	theme	mass-production	787:801	arg1	molding					767:773	micro-injection molding	751:773	micro-injection molding	751:773	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	1	75	theme	versatile	214:222	arg1	molding					179:185	sacrificial molding	167:185	sacrificial molding of hydrogel monoliths	167:207	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	1	75	theme	versatile	214:222	arg1	technique					224:232	a versatile technique	212:232	a versatile technique for creating 3D molds to control tissue morphology	212:283	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	5	76	theme	polymer	1015:1021	arg1	manufacturability					1035:1051	the polymer composite's manufacturability	1011:1051	the polymer composite's manufacturability	1011:1051	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	10	77	theme	hydrogel	1965:1972	arg1	monoliths					1974:1982	hydrogel monoliths	1965:1982	hydrogel monoliths	1965:1982	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	3	78	theme	poorer	534:539	arg1	performance					541:551	poorer performance	534:551	poorer performance	534:551	However, they display poorer performance in controlling the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate.
31028908	8	79	theme	cylindrical	1658:1668	arg1	morphology					1670:1679	a microscale, biomimetic cylindrical morphology	1633:1679	a microscale, biomimetic cylindrical morphology	1633:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	6	80	theme	average	1330:1336	arg1	error					1338:1342	average error	1330:1342	6.4 ± 7.2% average error	1319:1342	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	1	81	theme	3D	247:248	arg1	molds					250:254	3D molds	247:254	3D molds	247:254	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	4	82	theme	curing	870:875	arg1	hydrogels					877:885	bulk curing hydrogels	865:885	bulk curing hydrogels	865:885	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	83	theme	cross-linked	2616:2627	arg1	hydrogels					2638:2646	ionically cross-linked alginate hydrogels	2606:2646	ionically cross-linked alginate hydrogels	2606:2646	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	11	84	dep	polymerizing	2200:2211	arg1	curing					2219:2224	curing	2219:2224	curing	2219:2224	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	10	85	theme	versatile	1989:1997	arg1	molding					1954:1960	Sacrificial molding	1942:1960	Sacrificial molding of hydrogel monoliths	1942:1982	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	10	85	theme	versatile	1989:1997	arg1	technique					1999:2007	a versatile technique	1987:2007	a versatile technique for creating 3D molds for tissue engineering applications	1987:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	15	86	theme	scalable	2951:2958	arg1	customization					2963:2975	scalable 3D customization	2951:2975	scalable 3D customization of hydrogel internal architecture	2951:3009	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	2	87	theme	Previous	286:293	arg1	templates					307:315	Previous sacrificial templates	286:315	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing	286:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	3	88	dep	limited	594:600	arg1	cross-linked					613:624	cross-linked	613:624	cross-linked	613:624	However, they display poorer performance in controlling the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate.
31028908	11	89	theme	serial	2113:2118	arg1	processes					2120:2128	serial processes	2113:2128	serial processes	2113:2128	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	6	90	dep	%	1328:1328	arg1	error					1338:1342	average error	1330:1342	6.4 ± 7.2% average error	1319:1342	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	10	91	theme	3D	2022:2023	arg1	molds					2025:2029	3D molds	2022:2029	3D molds for tissue engineering applications	2022:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	3	92	theme	limited	594:600	arg1	hydrogels					626:634	diffusion limited, ionically cross-linked hydrogels	584:634	diffusion limited, ionically cross-linked hydrogels	584:634	However, they display poorer performance in controlling the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate.
31028908	3	92	theme	limited	594:600	arg1	alginate					645:652	alginate	645:652	alginate	645:652	However, they display poorer performance in controlling the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate.
31028908	9	93	theme	sacrificial	1760:1770	arg1	molding					1772:1778	hydrogel sacrificial molding	1751:1778	hydrogel sacrificial molding	1751:1778	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	12	94	theme	poor	2259:2262	arg1	performance					2264:2274	poor performance	2259:2274	poor performance	2259:2274	However, they display poor performance in molding geometry within diffusion limited, ionically cross-linked hydrogels, e.g. alginate.
31028908	0	95	theme	internal	108:115	arg1	architecture					117:128	3D hydrogel internal architecture	96:128	3D hydrogel internal architecture	96:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	15	96	link	hPSC-derived	2847:2858	arg1	organoids					2876:2884	hPSC-derived neuroepithelial organoids	2847:2884	hPSC-derived neuroepithelial organoids	2847:2884	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	1	97	theme	hydrogel	190:197	arg1	monoliths					199:207	hydrogel monoliths	190:207	hydrogel monoliths	190:207	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	13	98	theme	poly	2394:2397	arg1	templates					2427:2435	poly(vinyl alcohol)-calcium salt templates	2394:2435	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process,	2394:2525	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	98	theme	poly	2394:2397	arg1	PVOH-Ca					2438:2444	PVOH-Ca	2438:2444	PVOH-Ca	2438:2444	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	10	99	theme	engineering	2042:2052	arg1	applications					2054:2065	tissue engineering applications	2035:2065	tissue engineering applications	2035:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	5	100	theme	calcium	1097:1103	arg1	quantity					1085:1092	the quantity	1081:1092	the quantity of calcium released upon template dissolution	1081:1138	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	5	100	theme	calcium	1097:1103	arg1	manufacturability					1035:1051	the polymer composite's manufacturability	1011:1051	the polymer composite's manufacturability	1011:1051	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	5	100	theme	calcium	1097:1103	arg1	properties					1065:1074	mechanical properties	1054:1074	mechanical properties	1054:1074	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	3	101	theme	hydrogels	626:634	arg1	geometry					572:579	the geometry	568:579	the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate	568:652	However, they display poorer performance in controlling the geometry of diffusion limited, ionically cross-linked hydrogels, such as alginate.
31028908	12	102	dep	limited	2313:2319	arg1	cross-linked					2332:2343	cross-linked	2332:2343	cross-linked	2332:2343	However, they display poor performance in molding geometry within diffusion limited, ionically cross-linked hydrogels, e.g. alginate.
31028908	6	103	theme	microscale	1262:1271	arg1	geometries					1281:1290	microscale channel geometries	1262:1290	microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error)	1262:1343	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	2	104	used	used	415:418	arg2	templates					307:315	Previous sacrificial templates	286:315	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing	286:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	13	105	theme	curing	2585:2590	arg1	hydrogels					2592:2600	bulk curing hydrogels	2580:2600	bulk curing hydrogels	2580:2600	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	5	106	theme	Calcium	934:940	arg1	factor					990:995	a critical factor	979:995	a critical factor	979:995	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	5	106	theme	Calcium	934:940	arg1	solubility					947:956	Calcium salt solubility	934:956	Calcium salt solubility	934:956	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	4	107	theme	cross-linked	901:912	arg1	hydrogels					923:931	ionically cross-linked alginate hydrogels	891:931	ionically cross-linked alginate hydrogels	891:931	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	108	theme	-calcium	703:710	arg1	PVOH-Ca					728:734	PVOH-Ca	728:734	PVOH-Ca	728:734	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	108	theme	-calcium	703:710	arg1	templates					717:725	poly(vinyl alcohol)-calcium salt templates	684:725	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process,	684:810	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	15	109	theme	hydrogel	2980:2987	arg1	architecture					2998:3009	hydrogel internal architecture	2980:3009	hydrogel internal architecture	2980:3009	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	10	110	theme	SIGNIFICANCE	1928:1939	arg1	STATEMENT					1915:1923	STATEMENT	1915:1923	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.	1915:2066	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	8	111	theme	hydrogel	1532:1539	arg1	molds					1541:1545	hydrogel molds	1532:1545	hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1532:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	7	112	theme	channel	1395:1401	arg1	networks					1403:1410	mold 3D channel networks	1387:1410	mold 3D channel networks within alginate hydrogels	1387:1436	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	4	113	theme	templates	717:725	arg1	use					677:679	the use	673:679	the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels	673:931	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	0	114	theme	salt	52:55	arg1	templates					57:65	poly(vinyl alcohol)-calcium salt templates	24:65	poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture	24:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	11	115	theme	sacrificial	2077:2087	arg1	materials					2089:2097	Previous sacrificial materials	2068:2097	Previous sacrificial materials fabricated by serial processes	2068:2128	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	13	116	theme	-calcium	2413:2420	arg1	templates					2427:2435	poly(vinyl alcohol)-calcium salt templates	2394:2435	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process,	2394:2525	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	116	theme	-calcium	2413:2420	arg1	PVOH-Ca					2438:2444	PVOH-Ca	2438:2444	PVOH-Ca	2438:2444	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	7	117	theme	mold	1387:1390	arg1	networks					1403:1410	mold 3D channel networks	1387:1410	mold 3D channel networks within alginate hydrogels	1387:1436	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	1	118	theme	engineering	141:151	arg1	applications					153:164	tissue engineering applications	134:164	tissue engineering applications	134:164	In tissue engineering applications, sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds to control tissue morphology.
31028908	11	119	used	used	2140:2143	arg2	materials					2089:2097	Previous sacrificial materials	2068:2097	Previous sacrificial materials fabricated by serial processes	2068:2128	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	14	120	theme	alginate	2731:2738	arg1	hydrogels					2740:2748	alginate hydrogels	2731:2748	alginate hydrogels	2731:2748	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	9	121	theme	complex	1829:1835	arg1	architecture					1857:1868	complex internal microscale architecture	1829:1868	complex internal microscale architecture for diverse tissue engineering applications	1829:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	8	122	theme	human	1563:1567	arg1	hESC					1590:1593	hESC	1590:1593	hESC	1590:1593	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	8	122	theme	human	1563:1567	arg1	cell					1584:1587	human embryonic stem cell	1563:1587	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	5	123	theme	critical	981:988	arg1	factor					990:995	a critical factor	979:995	a critical factor	979:995	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	5	123	theme	critical	981:988	arg1	solubility					947:956	Calcium salt solubility	934:956	Calcium salt solubility	934:956	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	9	124	theme	microscale	1846:1855	arg1	architecture					1857:1868	complex internal microscale architecture	1829:1868	complex internal microscale architecture for diverse tissue engineering applications	1829:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	8	125	theme	stem	1579:1582	arg1	hESC					1590:1593	hESC	1590:1593	hESC	1590:1593	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	8	125	theme	stem	1579:1582	arg1	cell					1584:1587	human embryonic stem cell	1563:1587	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	4	126	theme	micro-injection	751:765	arg1	process					803:809	a parallel mass-production process	776:809	a parallel mass-production process	776:809	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	126	theme	micro-injection	751:765	arg1	molding					767:773	micro-injection molding	751:773	micro-injection molding	751:773	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	0	127	theme	3D	96:97	arg1	architecture					117:128	3D hydrogel internal architecture	96:128	3D hydrogel internal architecture	96:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	13	128	theme	salt	2422:2425	arg1	templates					2427:2435	poly(vinyl alcohol)-calcium salt templates	2394:2435	poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process,	2394:2525	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	128	theme	salt	2422:2425	arg1	PVOH-Ca					2438:2444	PVOH-Ca	2438:2444	PVOH-Ca	2438:2444	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	7	129	theme	PVOH-Ca	1366:1372	arg1	templates					1374:1382	modular PVOH-Ca templates	1358:1382	modular PVOH-Ca templates	1358:1382	Assembly of modular PVOH-Ca templates to mold 3D channel networks within alginate hydrogels is presented to demonstrate engineering scalability.
31028908	13	130	theme	alginate	2629:2636	arg1	hydrogels					2638:2646	ionically cross-linked alginate hydrogels	2606:2646	ionically cross-linked alginate hydrogels	2606:2646	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	9	131	theme	tissue	1882:1887	arg1	applications					1901:1912	diverse tissue engineering applications	1874:1912	diverse tissue engineering applications	1874:1912	Thus, injection molded PVOH-Ca templates facilitate customization of hydrogel sacrificial molding, which can be used to generate 3D hydrogels with complex internal microscale architecture for diverse tissue engineering applications.
31028908	2	132	theme	extrusion/fiber	384:398	arg1	drawing					400:406	thermal extrusion/fiber drawing	376:406	thermal extrusion/fiber drawing	376:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	0	133	theme	poly	24:27	arg1	templates					57:65	poly(vinyl alcohol)-calcium salt templates	24:65	poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture	24:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	6	134	theme	channel	1273:1279	arg1	geometries					1281:1290	microscale channel geometries	1262:1290	microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error)	1262:1343	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	2	135	theme	internal	440:447	arg1	geometries					449:458	mold internal geometries	435:458	mold internal geometries within rapidly polymerizing, bulk curing hydrogels	435:509	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	8	136	theme	neuroepithelial	1604:1618	arg1	organoids					1620:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids	1563:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	2	137	theme	thermal	376:382	arg1	drawing					400:406	thermal extrusion/fiber drawing	376:406	thermal extrusion/fiber drawing	376:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	0	138	theme	vinyl	29:33	arg1	poly					24:27	poly	24:27	poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture	24:128	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	0	138	theme	vinyl	29:33	arg1	alcohol					35:41	vinyl alcohol	29:41	vinyl alcohol	29:41	Micro-injection molded, poly(vinyl alcohol)-calcium salt templates for precise customization of 3D hydrogel internal architecture.
31028908	13	139	theme	templates	2427:2435	arg1	use					2387:2389	the use	2383:2389	the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels	2383:2646	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	140	theme	unparalleled	2489:2500	arg1	process					2518:2524	an unparalleled mass-production process	2486:2524	an unparalleled mass-production process	2486:2524	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	140	theme	unparalleled	2489:2500	arg1	molding					2477:2483	micro-injection molding	2461:2483	micro-injection molding	2461:2483	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	2	141	theme	bulk	489:492	arg1	curing					494:499	bulk curing	489:499	bulk curing	489:499	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	10	142	theme	Sacrificial	1942:1952	arg1	molding					1954:1960	Sacrificial molding	1942:1960	Sacrificial molding of hydrogel monoliths	1942:1982	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	10	142	theme	Sacrificial	1942:1952	arg1	technique					1999:2007	a versatile technique	1987:2007	a versatile technique for creating 3D molds for tissue engineering applications	1987:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	15	143	theme	PVOH-Ca	2899:2905	arg1	designs					2916:2922	modular PVOH-Ca template designs	2891:2922	modular PVOH-Ca template designs	2891:2922	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	15	144	theme	organoids	2876:2884	arg1	morphology					2833:2842	the morphology	2829:2842	the morphology of hPSC-derived neuroepithelial organoids	2829:2884	Moreover, we demonstrate its use to engineer the morphology of hPSC-derived neuroepithelial organoids, and modular PVOH-Ca template designs can be assembled to enable scalable 3D customization of hydrogel internal architecture.
31028908	14	145	theme	PVOH-Ca	2674:2680	arg1	templates					2682:2690	the PVOH-Ca templates	2670:2690	the PVOH-Ca templates	2670:2690	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	5	146	theme	mechanical	1054:1063	arg1	properties					1065:1074	mechanical properties	1054:1074	mechanical properties	1054:1074	Calcium salt solubility was discovered to be a critical factor in optimizing the polymer composite's manufacturability, mechanical properties, and the quantity of calcium released upon template dissolution.
31028908	2	147	theme	polymerizing	475:486	arg1	hydrogels					501:509	rapidly polymerizing, bulk curing hydrogels	467:509	hydrogels	501:509	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	8	148	theme	microscale	1635:1644	arg1	morphology					1670:1679	a microscale, biomimetic cylindrical morphology	1633:1679	a microscale, biomimetic cylindrical morphology	1633:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
31028908	13	149	theme	micro-injection	2461:2475	arg1	process					2518:2524	an unparalleled mass-production process	2486:2524	an unparalleled mass-production process	2486:2524	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	13	149	theme	micro-injection	2461:2475	arg1	molding					2477:2483	micro-injection molding	2461:2483	micro-injection molding	2461:2483	We describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, an unparalleled mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	11	150	theme	Previous	2068:2075	arg1	materials					2089:2097	Previous sacrificial materials	2068:2097	Previous sacrificial materials fabricated by serial processes	2068:2128	Previous sacrificial materials fabricated by serial processes have been used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	6	151	theme	computed	1158:1165	arg1	CT					1179:1180	CT	1179:1180	CT	1179:1180	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	6	151	theme	computed	1158:1165	arg1	tomography					1167:1176	computed tomography	1158:1176	computed tomography (CT)	1158:1181	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	10	152	theme	monoliths	1974:1982	arg1	molding					1954:1960	Sacrificial molding	1942:1960	Sacrificial molding of hydrogel monoliths	1942:1982	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	10	152	theme	monoliths	1974:1982	arg1	technique					1999:2007	a versatile technique	1987:2007	a versatile technique for creating 3D molds for tissue engineering applications	1987:2065	STATEMENT OF SIGNIFICANCE: Sacrificial molding of hydrogel monoliths is a versatile technique for creating 3D molds for tissue engineering applications.
31028908	4	153	theme	parallel	778:785	arg1	process					803:809	a parallel mass-production process	776:809	a parallel mass-production process	776:809	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	4	153	theme	parallel	778:785	arg1	molding					767:773	micro-injection molding	751:773	micro-injection molding	751:773	Here, we describe the use of poly(vinyl alcohol)-calcium salt templates (PVOH-Ca) fabricated by micro-injection molding, a parallel mass-production process, to conveniently cast internal geometries within both bulk curing hydrogels and ionically cross-linked alginate hydrogels.
31028908	2	154	theme	serial	331:336	arg1	casting					364:370	solvent casting	356:370	solvent casting	356:370	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	2	154	theme	serial	331:336	arg1	processes					338:346	serial processes	331:346	serial processes such as solvent casting and thermal extrusion/fiber drawing	331:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	2	154	theme	serial	331:336	arg1	drawing					400:406	thermal extrusion/fiber drawing	376:406	thermal extrusion/fiber drawing	376:406	Previous sacrificial templates fabricated by serial processes such as solvent casting and thermal extrusion/fiber drawing can be used to effectively mold internal geometries within rapidly polymerizing, bulk curing hydrogels.
31028908	14	155	theme	Calcium	2649:2655	arg1	release					2657:2663	Calcium release	2649:2663	Calcium release from the PVOH-Ca templates	2649:2690	Calcium release from the PVOH-Ca templates enables precise sacrificial molding of alginate hydrogels and the process is biocompatible.
31028908	6	156	theme	Metrological	1141:1152	arg1	analysis					1183:1190	Metrological and computed tomography (CT) analysis	1141:1190	Metrological and computed tomography (CT) analysis	1141:1190	Metrological and computed tomography (CT) analysis showed that the template's calcium release enables precise casting of microscale channel geometries within alginate hydrogels (6.4 ± 7.2% average error).
31028908	8	157	theme	morphology	1670:1679	arg1	organoids					1620:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids	1563:1628	human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology	1563:1679	Moreover, the platform is used to create hydrogel molds for engineering human embryonic stem cell (hESC)-derived neuroepithelial organoids of a microscale, biomimetic cylindrical morphology.
29529511	1	0	theme	polyimide	357:365	arg1	substrate					367:375	a flexible polyimide substrate	346:375	a flexible polyimide substrate using simple low-cost fabrication processes	346:419	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	2	1	theme	sensor	685:690	arg1	application					692:702	a human sweat-based wearable glucose sensor application	648:702	a human sweat-based wearable glucose sensor application	648:702	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	1	2	theme	working	256:262	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	2	theme	working	256:262	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	2	3	theme	working	619:625	arg1	electrode					627:635	the working electrode	615:635	the working electrode	615:635	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	2	4	theme	glucose	677:683	arg1	sensor					685:690	a human sweat-based wearable glucose sensor	648:690	a human sweat-based wearable glucose sensor application	648:702	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	3	5	theme	time	905:908	arg1	sensitivity					860:870	a sensitivity	858:870	a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99)	858:942	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	0	6	theme	reduced	109:115	arg1	electrode					146:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	3	7	theme	detection	788:796	arg1	range					798:802	a detection range	786:802	a detection range of 0-2.4 mM (covers the glucose range in sweat)	786:850	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	2	8	theme	wearable	668:675	arg1	sensor					685:690	a human sweat-based wearable glucose sensor	648:690	a human sweat-based wearable glucose sensor application	648:702	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	6	9	theme	human	1312:1316	arg1	sensors					1354:1360	human sweat-based electrochemical glucose sensors	1312:1360	human sweat-based electrochemical glucose sensors	1312:1360	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	3	10	theme	high	922:925	arg1	0.99					938:941	0.99	938:941	0.99	938:941	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	3	10	theme	high	922:925	arg1	linearity					927:935	high linearity	922:935	high linearity (0.99)	922:942	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	0	11	theme	micro-patterned	93:107	arg1	electrode					146:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	1	12	theme	simple	383:388	arg1	processes					411:419	simple low-cost fabrication processes	383:419	simple low-cost fabrication processes	383:419	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	2	13	theme	sweat-based	656:666	arg1	sensor					685:690	a human sweat-based wearable glucose sensor	648:690	a human sweat-based wearable glucose sensor application	648:702	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	5	14	theme	low	1128:1130	arg1	concentrations					1140:1153	low glucose concentrations	1128:1153	low glucose concentrations	1128:1153	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	1	15	theme	high	277:280	arg1	quality					282:288	high quality	277:288	high quality	277:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	0	16	theme	oxide	126:130	arg1	electrode					146:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	1	17	theme	low-cost	390:397	arg1	processes					411:419	simple low-cost fabrication processes	383:419	simple low-cost fabrication processes	383:419	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	5	18	theme	human	1005:1009	arg1	samples					1032:1038	The human sweat/mixing glucose samples	1001:1038	The human sweat/mixing glucose samples initially used for testing	1001:1065	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	2	19	theme	platinum	431:438	arg1	nanoparticles					446:458	Gold and platinum alloy nanoparticles	422:458	nanoparticles	446:458	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	0	20	theme	graphene	117:124	arg1	electrode					146:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	1	21	theme	fabrication	399:409	arg1	processes					411:419	simple low-cost fabrication processes	383:419	simple low-cost fabrication processes	383:419	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	22	from	microfabricated	307:321	arg1	study					187:191	this study	182:191	this study	182:191	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	5	23	theme	sweat/mixing	1011:1022	arg1	samples					1032:1038	The human sweat/mixing glucose samples	1001:1038	The human sweat/mixing glucose samples initially used for testing	1001:1065	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	0	24	theme	wearable	2:9	arg1	sensor					35:40	A wearable electrochemical glucose sensor	0:40	A wearable electrochemical glucose sensor based on simple and low-cost fabrication	0:81	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	2	25	theme	oxidase	551:557	arg1	composites					559:568	chitosan-glucose oxidase composites	534:568	chitosan-glucose oxidase composites	534:568	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	2	26	theme	Gold	422:425	arg1	nanoparticles					446:458	Gold and platinum alloy nanoparticles	422:458	nanoparticles	446:458	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	1	27	theme	quality	282:288	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	27	theme	quality	282:288	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	6	28	theme	glucose	1346:1352	arg1	sensors					1354:1360	human sweat-based electrochemical glucose sensors	1312:1360	human sweat-based electrochemical glucose sensors	1312:1360	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	29	theme	working	1230:1236	arg1	electrode					1238:1246	the proposed nanostructured composite flexible working electrode	1183:1246	the proposed nanostructured composite flexible working electrode	1183:1246	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	29	theme	working	1230:1236	arg1	promising					1283:1291	promising	1283:1291	promising	1283:1291	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	1	30	theme	reduced	196:202	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	30	theme	reduced	196:202	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	0	31	theme	glucose	27:33	arg1	sensor					35:40	A wearable electrochemical glucose sensor	0:40	A wearable electrochemical glucose sensor based on simple and low-cost fabrication	0:81	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	2	32	theme	electrode	627:635	arg1	surface					604:610	the modified surface	591:610	the modified surface of the working electrode	591:635	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	5	33	theme	acceptable	1077:1086	arg1	performance					1098:1108	acceptable detection performance	1077:1108	acceptable detection performance	1077:1108	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	3	34	theme	short	890:894	arg1	20 s					911:914	20 s	911:914	20 s	911:914	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	3	34	theme	short	890:894	arg1	time					905:908	a short response time	888:908	a short response time (20 s)	888:915	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	1	35	theme	graphene	204:211	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	35	theme	graphene	204:211	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	0	36	theme	electrochemical	11:25	arg1	sensor					35:40	A wearable electrochemical glucose sensor	0:40	A wearable electrochemical glucose sensor based on simple and low-cost fabrication	0:81	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	3	37	theme	glucose	828:834	arg1	range					836:840	the glucose range	824:840	the glucose range in sweat	824:849	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	0	38	theme	nanocomposite	132:144	arg1	electrode					146:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	micro-patterned reduced graphene oxide nanocomposite electrode	93:154	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	3	39	theme	excellent	740:748	arg1	response					763:770	excellent amperometric response	740:770	excellent amperometric response to glucose	740:781	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	6	40	theme	composite	1211:1219	arg1	electrode					1238:1246	the proposed nanostructured composite flexible working electrode	1183:1246	the proposed nanostructured composite flexible working electrode	1183:1246	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	40	theme	composite	1211:1219	arg1	promising					1283:1291	promising	1283:1291	promising	1283:1291	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	1	41	theme	oxide	213:217	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	41	theme	oxide	213:217	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	2	42	theme	chitosan-glucose	534:549	arg1	composites					559:568	chitosan-glucose oxidase composites	534:568	chitosan-glucose oxidase composites	534:568	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	0	43	theme	flexible	159:166	arg1	substrate					168:176	flexible substrate	159:176	flexible substrate	159:176	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	3	44	theme	amperometric	750:761	arg1	response					763:770	excellent amperometric response	740:770	excellent amperometric response to glucose	740:781	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	6	45	theme	electrochemical	1330:1344	arg1	sensors					1354:1360	human sweat-based electrochemical glucose sensors	1312:1360	human sweat-based electrochemical glucose sensors	1312:1360	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	3	46	dep	0-2.4 mM	807:814	arg1	covers					817:822	covers	817:822	covers the glucose range in sweat	817:849	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	5	47	theme	glucose	1132:1138	arg1	concentrations					1140:1153	low glucose concentrations	1128:1153	low glucose concentrations	1128:1153	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	6	48	theme	sweat-based	1318:1328	arg1	sensors					1354:1360	human sweat-based electrochemical glucose sensors	1312:1360	human sweat-based electrochemical glucose sensors	1312:1360	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	1	49	theme	rGO	220:222	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	49	theme	rGO	220:222	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	0	50	theme	simple	51:56	arg1	fabrication					71:81	simple and low-cost fabrication	51:81	simple and low-cost fabrication	51:81	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	2	51	theme	human	650:654	arg1	sensor					685:690	a human sweat-based wearable glucose sensor	648:690	a human sweat-based wearable glucose sensor application	648:702	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	2	52	theme	modified	595:602	arg1	surface					604:610	the modified surface	591:610	the modified surface of the working electrode	591:635	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	3	53	theme	0-2.4 mM	807:814	arg1	range					798:802	a detection range	786:802	a detection range of 0-2.4 mM (covers the glucose range in sweat)	786:850	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	6	54	theme	nanostructured	1196:1209	arg1	electrode					1238:1246	the proposed nanostructured composite flexible working electrode	1183:1246	the proposed nanostructured composite flexible working electrode	1183:1246	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	54	theme	nanostructured	1196:1209	arg1	promising					1283:1291	promising	1283:1291	promising	1283:1291	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	2	55	theme	rGO	518:520	arg1	surface					522:528	the microfabricated rGO surface	498:528	the microfabricated rGO surface	498:528	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	5	56	theme	detection	1088:1096	arg1	performance					1098:1108	acceptable detection performance	1077:1108	acceptable detection performance	1077:1108	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	3	57	from	range	836:840	arg1	sweat					845:849	sweat	845:849	sweat	845:849	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	6	58	theme	proposed	1187:1194	arg1	electrode					1238:1246	the proposed nanostructured composite flexible working electrode	1183:1246	the proposed nanostructured composite flexible working electrode	1183:1246	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	58	theme	proposed	1187:1194	arg1	promising					1283:1291	promising	1283:1291	promising	1283:1291	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	5	59	theme	glucose	1024:1030	arg1	samples					1032:1038	The human sweat/mixing glucose samples	1001:1038	The human sweat/mixing glucose samples initially used for testing	1001:1065	The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations.
29529511	6	60	theme	flexible	1221:1228	arg1	electrode					1238:1246	the proposed nanostructured composite flexible working electrode	1183:1246	the proposed nanostructured composite flexible working electrode	1183:1246	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	6	60	theme	flexible	1221:1228	arg1	promising					1283:1291	promising	1283:1291	promising	1283:1291	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	3	61	theme	48 μA/mMcm2	875:885	arg1	sensitivity					860:870	a sensitivity	858:870	a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99)	858:942	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	3	62	theme	fabricated	709:718	arg1	biosensor					720:728	The fabricated biosensor	705:728	The fabricated biosensor	705:728	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	3	63	theme	response	896:903	arg1	20 s					911:914	20 s	911:914	20 s	911:914	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	3	63	theme	response	896:903	arg1	time					905:908	a short response time	888:908	a short response time (20 s)	888:915	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	4	64	theme	detection	949:957	arg1	limit					959:963	The detection limit	945:963	The detection limit for glucose	945:975	The detection limit for glucose was calculated as 5 µm.
29529511	4	64	theme	detection	949:957	arg1	5 µm					995:998	5 µm	995:998	5 µm	995:998	The detection limit for glucose was calculated as 5 µm.
29529511	1	65	theme	-based	224:229	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	65	theme	-based	224:229	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	0	66	theme	low-cost	62:69	arg1	fabrication					71:81	simple and low-cost fabrication	51:81	simple and low-cost fabrication	51:81	A wearable electrochemical glucose sensor based on simple and low-cost fabrication supported micro-patterned reduced graphene oxide nanocomposite electrode on flexible substrate.
29529511	2	67	theme	alloy	440:444	arg1	nanoparticles					446:458	Gold and platinum alloy nanoparticles	422:458	nanoparticles	446:458	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	6	68	theme	fabrication	1252:1262	arg1	process					1264:1270	fabrication process	1252:1270	fabrication process	1252:1270	These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.
29529511	1	69	theme	nanostructured	231:244	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	69	theme	nanostructured	231:244	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	70	theme	flexible	348:355	arg1	substrate					367:375	a flexible polyimide substrate	346:375	a flexible polyimide substrate using simple low-cost fabrication processes	346:419	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	71	from	study	187:191	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	71	from	study	187:191	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	2	72	theme	microfabricated	502:516	arg1	surface					522:528	the microfabricated rGO surface	498:528	the microfabricated rGO surface	498:528	Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application.
29529511	3	73	theme	linearity	927:935	arg1	sensitivity					860:870	a sensitivity	858:870	a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99)	858:942	The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0-2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm2, a short response time (20 s), and high linearity (0.99).
29529511	1	74	theme	composite	246:254	arg1	electrode					264:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode	194:272	a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality	194:288	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29529511	1	74	theme	composite	246:254	arg1	microfabricated					307:321	microfabricated	307:321	microfabricated	307:321	In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes.
29655888	0	0	theme	activated	74:82	arg1	bentonite					84:92	activated bentonite	74:92	activated bentonite	74:92	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	1	1	from	solution	338:345	arg1	removal					286:292	the removal	282:292	the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution	282:345	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	8	2	theme	monolayer	1235:1243	arg1	adsorption					1257:1266	a monolayer homogeneous adsorption	1233:1266	a monolayer homogeneous adsorption	1233:1266	The Langmuir isotherm model fitted well the isotherm data, indicating a monolayer homogeneous adsorption.
29655888	2	3	theme	cationic	384:391	arg1	trimethylammonium					443:459	hexadecyl trimethylammonium	433:459	hexadecyl trimethylammonium	433:459	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	3	theme	cationic	384:391	arg1	surfactants					393:403	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	3	theme	cationic	384:391	arg1	phenyltrimethylammonium					465:487	phenyltrimethylammonium	465:487	phenyltrimethylammonium	465:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	3	theme	cationic	384:391	arg1	octadecyltrimethylammonium					405:430	octadecyltrimethylammonium	405:430	octadecyltrimethylammonium	405:430	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	0	4	from	medium	176:181	arg1	removal					133:139	removal	133:139	removal of 2,4-dichlorophenol from aqueous medium	133:181	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	9	5	theme	adsorption	1319:1328	arg1	capacity					1330:1337	relatively high adsorption capacity	1303:1337	relatively high adsorption capacity of 142 to 391 mg/g founded by this model	1303:1378	The prepared adsorbents exhibited relatively high adsorption capacity of 142 to 391 mg/g founded by this model.
29655888	6	6	theme	2,4DCP	937:942	arg1	amount					927:932	the amount	923:932	the amount of 2,4DCP	923:942	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	6	6	theme	2,4DCP	937:942	arg1	2,4DCP					937:942	2,4DCP	937:942	2,4DCP	937:942	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	8	7	theme	Langmuir	1167:1174	arg1	model					1185:1189	The Langmuir isotherm model	1163:1189	The Langmuir isotherm model	1163:1189	The Langmuir isotherm model fitted well the isotherm data, indicating a monolayer homogeneous adsorption.
29655888	9	8	theme	high	1314:1317	arg1	capacity					1330:1337	relatively high adsorption capacity	1303:1337	relatively high adsorption capacity of 142 to 391 mg/g founded by this model	1303:1378	The prepared adsorbents exhibited relatively high adsorption capacity of 142 to 391 mg/g founded by this model.
29655888	4	9	theme	cationic	674:681	arg1	surfactants					683:693	The intercalated cationic surfactants	657:693	The intercalated cationic surfactants	657:693	The intercalated cationic surfactants were characterized by Fourier transform infrared spectroscopy (FTIR).
29655888	6	10	theme	contact	993:999	arg1	time					1001:1004	contact time	993:1004	contact time	993:1004	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	3	11	theme	structural	619:628	arg1	properties					630:639	the structural properties	615:639	the structural properties of the samples	615:654	X-ray diffraction was used to study the change in the structural properties of the samples.
29655888	4	12	theme	intercalated	661:672	arg1	surfactants					683:693	The intercalated cationic surfactants	657:693	The intercalated cationic surfactants	657:693	The intercalated cationic surfactants were characterized by Fourier transform infrared spectroscopy (FTIR).
29655888	0	13	theme	calcium	112:118	arg1	alginate					120:127	calcium alginate	112:127	calcium alginate	112:127	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	9	14	theme	142	1342:1344	arg1	capacity					1330:1337	relatively high adsorption capacity	1303:1337	relatively high adsorption capacity of 142 to 391 mg/g founded by this model	1303:1378	The prepared adsorbents exhibited relatively high adsorption capacity of 142 to 391 mg/g founded by this model.
29655888	5	15	theme	initial	869:875	arg1	concentration					884:896	initial 2,4DCP concentration	869:896	initial 2,4DCP concentration	869:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	8	16	theme	isotherm	1176:1183	arg1	model					1185:1189	The Langmuir isotherm model	1163:1189	The Langmuir isotherm model	1163:1189	The Langmuir isotherm model fitted well the isotherm data, indicating a monolayer homogeneous adsorption.
29655888	2	17	dep	surfactants	393:403	arg1	trimethylammonium					443:459	hexadecyl trimethylammonium	433:459	hexadecyl trimethylammonium	433:459	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	17	dep	surfactants	393:403	arg1	surfactants					393:403	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	17	dep	surfactants	393:403	arg1	phenyltrimethylammonium					465:487	phenyltrimethylammonium	465:487	phenyltrimethylammonium	465:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	17	dep	surfactants	393:403	arg1	octadecyltrimethylammonium					405:430	octadecyltrimethylammonium	405:430	octadecyltrimethylammonium	405:430	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	5	18	theme	contact	835:841	arg1	time					843:846	contact time	835:846	contact time	835:846	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	6	19	theme	initial	970:976	arg1	concentration					978:990	initial concentration	970:990	initial concentration	970:990	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	2	20	theme	hexadecyl	433:441	arg1	trimethylammonium					443:459	hexadecyl trimethylammonium	433:459	hexadecyl trimethylammonium	433:459	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	20	theme	hexadecyl	433:441	arg1	surfactants					393:403	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	1	21	theme	2,4-dichlorophenol	297:314	arg1	removal					286:292	the removal	282:292	the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution	282:345	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	3	22	theme	samples	648:654	arg1	properties					630:639	the structural properties	615:639	the structural properties of the samples	615:654	X-ray diffraction was used to study the change in the structural properties of the samples.
29655888	2	23	theme	calcium	513:519	arg1	alginate					521:528	calcium alginate	513:528	calcium alginate	513:528	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	8	24	theme	isotherm	1207:1214	arg1	data					1216:1219	the isotherm data	1203:1219	the isotherm data	1203:1219	The Langmuir isotherm model fitted well the isotherm data, indicating a monolayer homogeneous adsorption.
29655888	5	25	theme	2,4DCP	877:882	arg1	concentration					884:896	initial 2,4DCP concentration	869:896	initial 2,4DCP concentration	869:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	6	26	theme	2,4DCP	1064:1069	arg1	process					1053:1059	the adsorption process	1038:1059	the adsorption process of 2,4DCP onto composites	1038:1085	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	6	26	theme	2,4DCP	1064:1069	arg1	endothermic					1090:1100	endothermic	1090:1100	endothermic	1090:1100	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	5	27	theme	various	798:804	arg1	temperature					849:859	temperature	849:859	temperature	849:859	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	27	theme	various	798:804	arg1	pH					862:863	pH	862:863	pH	862:863	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	27	theme	various	798:804	arg1	time					843:846	contact time	835:846	contact time	835:846	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	27	theme	various	798:804	arg1	concentration					884:896	initial 2,4DCP concentration	869:896	initial 2,4DCP concentration	869:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	27	theme	various	798:804	arg1	parameters					816:825	various operating parameters	798:825	various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration	798:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	0	28	theme	low	40:42	arg1	beads					59:63	new low cost adsorbent beads	36:63	new low cost adsorbent beads based on activated bentonite	36:92	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	5	29	theme	operating	806:814	arg1	temperature					849:859	temperature	849:859	temperature	849:859	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	29	theme	operating	806:814	arg1	pH					862:863	pH	862:863	pH	862:863	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	29	theme	operating	806:814	arg1	time					843:846	contact time	835:846	contact time	835:846	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	29	theme	operating	806:814	arg1	concentration					884:896	initial 2,4DCP concentration	869:896	initial 2,4DCP concentration	869:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	5	29	theme	operating	806:814	arg1	parameters					816:825	various operating parameters	798:825	various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration	798:896	The adsorption was studied using various operating parameters such as contact time, temperature, pH and initial 2,4DCP concentration.
29655888	9	30	theme	prepared	1273:1280	arg1	adsorbents					1282:1291	The prepared adsorbents	1269:1291	The prepared adsorbents	1269:1291	The prepared adsorbents exhibited relatively high adsorption capacity of 142 to 391 mg/g founded by this model.
29655888	0	31	theme	new	36:38	arg1	beads					59:63	new low cost adsorbent beads	36:63	new low cost adsorbent beads based on activated bentonite	36:92	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	6	32	dep	increased	944:952	arg1	indicating					1022:1031	indicating	1022:1031	indicating that the adsorption process of 2,4DCP onto composites is endothermic	1022:1100	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	2	33	theme	composite	548:556	arg1	beads					558:562	adsorbent composite beads	538:562	adsorbent composite beads	538:562	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	1	34	theme	composites	221:230	arg1	beads					258:262	composites organo-bentonite/alginate beads	221:262	composites organo-bentonite/alginate beads	221:262	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	2	35	theme	adsorbent	538:546	arg1	beads					558:562	adsorbent composite beads	538:562	adsorbent composite beads	538:562	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	1	36	theme	aqueous	330:336	arg1	solution					338:345	aqueous solution	330:345	aqueous solution	330:345	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	0	37	theme	beads	59:63	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	0	37	theme	beads	59:63	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	4	38	dep	transform	725:733	arg1	infrared					735:742	infrared	735:742	transform infrared spectroscopy (FTIR)	725:762	The intercalated cationic surfactants were characterized by Fourier transform infrared spectroscopy (FTIR).
29655888	7	39	theme	pseudo-second-order	1133:1151	arg1	kinetics					1153:1160	pseudo-second-order kinetics	1133:1160	pseudo-second-order kinetics	1133:1160	Adsorption of 2,4DCP followed pseudo-second-order kinetics.
29655888	0	40	theme	cost	44:47	arg1	beads					59:63	new low cost adsorbent beads	36:63	new low cost adsorbent beads based on activated bentonite	36:92	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	0	41	theme	2,4-dichlorophenol	144:161	arg1	removal					133:139	removal	133:139	removal of 2,4-dichlorophenol from aqueous medium	133:181	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	4	42	dep	Fourier	717:723	arg1	transform					725:733	transform	725:733	transform infrared spectroscopy (FTIR)	725:762	The intercalated cationic surfactants were characterized by Fourier transform infrared spectroscopy (FTIR).
29655888	8	43	theme	homogeneous	1245:1255	arg1	adsorption					1257:1266	a monolayer homogeneous adsorption	1233:1266	a monolayer homogeneous adsorption	1233:1266	The Langmuir isotherm model fitted well the isotherm data, indicating a monolayer homogeneous adsorption.
29655888	7	44	theme	2,4DCP	1117:1122	arg1	Adsorption					1103:1112	Adsorption	1103:1112	Adsorption of 2,4DCP	1103:1122	Adsorption of 2,4DCP followed pseudo-second-order kinetics.
29655888	1	45	theme	organo-bentonite/alginate	232:256	arg1	beads					258:262	composites organo-bentonite/alginate beads	221:262	composites organo-bentonite/alginate beads	221:262	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	0	46	theme	aqueous	168:174	arg1	medium					176:181	aqueous medium	168:181	aqueous medium	168:181	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	3	47	used	used	587:590	arg2	diffraction					571:581	X-ray diffraction	565:581	X-ray diffraction	565:581	X-ray diffraction was used to study the change in the structural properties of the samples.
29655888	3	48	theme	X-ray	565:569	arg1	diffraction					571:581	X-ray diffraction	565:581	X-ray diffraction	565:581	X-ray diffraction was used to study the change in the structural properties of the samples.
29655888	1	49	theme	beads	258:262	arg1	potential					208:216	the potential	204:216	the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution	204:345	This study explored the potential of composites organo-bentonite/alginate beads as adsorbents for the removal of 2,4-dichlorophenol (2,4DCP) from aqueous solution.
29655888	2	50	mod	modified	370:377	arg3	octadecyltrimethylammonium					405:430	octadecyltrimethylammonium	405:430	octadecyltrimethylammonium	405:430	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	50	mod	modified	370:377	arg1	Bentonite					348:356	Bentonite	348:356	Bentonite	348:356	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	50	mod	modified	370:377	arg3	phenyltrimethylammonium					465:487	phenyltrimethylammonium	465:487	phenyltrimethylammonium	465:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	50	mod	modified	370:377	arg3	trimethylammonium					443:459	hexadecyl trimethylammonium	433:459	hexadecyl trimethylammonium	433:459	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	2	50	mod	modified	370:377	arg3	surfactants					393:403	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium	384:487	Bentonite was firstly modified with cationic surfactants octadecyltrimethylammonium, hexadecyl trimethylammonium and phenyltrimethylammonium, then encapsulated with calcium alginate to form adsorbent composite beads.
29655888	0	51	theme	adsorbent	49:57	arg1	beads					59:63	new low cost adsorbent beads	36:63	new low cost adsorbent beads based on activated bentonite	36:92	Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium.
29655888	3	52	from	change	605:610	arg1	properties					630:639	the structural properties	615:639	the structural properties of the samples	615:654	X-ray diffraction was used to study the change in the structural properties of the samples.
29655888	6	53	theme	adsorption	1042:1051	arg1	process					1053:1059	the adsorption process	1038:1059	the adsorption process of 2,4DCP onto composites	1038:1085	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
29655888	6	53	theme	adsorption	1042:1051	arg1	endothermic					1090:1100	endothermic	1090:1100	endothermic	1090:1100	The results showed that the amount of 2,4DCP increased with increasing initial concentration, contact time and temperature indicating that the adsorption process of 2,4DCP onto composites is endothermic.
31356999	4	0	theme	intermolecular	551:564	arg1	interaction					566:576	the intermolecular interaction	547:576	the intermolecular interaction	547:576	It was found that the intermolecular interaction and viscosity tend to increase with the increasing concentration of κ-carrageenan.
31356999	8	1	theme	Langmuir-Freundlich	1044:1062	arg1	model					1073:1077	the Langmuir-Freundlich isotherm model	1040:1077	the Langmuir-Freundlich isotherm model	1040:1077	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	8	1	theme	Langmuir-Freundlich	1044:1062	arg1	suitable					1087:1094	suitable	1087:1094	suitable	1087:1094	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	5	2	from	degree	674:679	arg1	solution					715:722	NaCl solution	710:722	NaCl solution	710:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	11	3	theme	hydrogen	1646:1653	arg1	bonding					1655:1661	hydrogen bonding	1646:1661	hydrogen bonding	1646:1661	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	3	4	theme	κ-Carrageenan/Sodium	356:375	arg1	hydrogels					412:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	9	5	theme	double-network	1273:1286	arg1	hydrogels					1288:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels exhibit excellent adsorption properties for CIP (229 mg/g).
31356999	8	6	theme	isotherm	1123:1130	arg1	data					1132:1135	the adsorption isotherm data	1108:1135	the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites	1108:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	10	7	theme	ion	1515:1517	arg1	concentration					1519:1531	ion concentration	1515:1531	ion concentration	1515:1531	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	10	8	theme	adsorption	1370:1379	arg1	capacity					1381:1388	The optimal adsorption capacity	1358:1388	The optimal adsorption capacity of κ-car/SA composite hydrogels	1358:1420	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	4	9	theme	κ-carrageenan	646:658	arg1	concentration					629:641	the increasing concentration	614:641	the increasing concentration of κ-carrageenan	614:658	It was found that the intermolecular interaction and viscosity tend to increase with the increasing concentration of κ-carrageenan.
31356999	3	10	theme	double-network	397:410	arg1	hydrogels					412:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	11	11	theme	composite	1715:1723	arg1	hydrogels					1725:1733	κ-car/SA composite hydrogels	1706:1733	κ-car/SA composite hydrogels	1706:1733	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	5	12	theme	decreasing	736:745	arg1	trend					747:751	a decreasing trend	734:751	a decreasing trend	734:751	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	11	13	theme	electrostatic	1671:1683	arg1	interactions					1685:1696	the electrostatic interactions	1667:1696	the electrostatic interactions between κ-car/SA composite hydrogels and CIP	1667:1741	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	2	14	theme	antibiotics	329:339	arg1	removal					318:324	the removal	314:324	the removal of antibiotics	314:339	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	7	15	theme	model	962:966	arg1	pollutant					968:976	the model pollutant	958:976	the model pollutant for testing the adsorption performance	958:1015	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	7	15	theme	model	962:966	arg1	Ciprofloxacin					926:938	Ciprofloxacin	926:938	Ciprofloxacin (CIP)	926:944	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	0	16	theme	adsorption	110:119	arg1	capacity					121:128	adsorption capacity	110:128	adsorption capacity	110:128	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	9	17	theme	excellent	1306:1314	arg1	properties					1327:1336	excellent adsorption properties	1306:1336	excellent adsorption properties for CIP (229 mg/g)	1306:1355	κ-car/SA composite double-network hydrogels exhibit excellent adsorption properties for CIP (229 mg/g).
31356999	11	18	theme	Zeta	1560:1563	arg1	test					1575:1578	the Zeta potential test	1556:1578	the Zeta potential test	1556:1578	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	11	19	dep	spectroscopy	1539:1550	arg1	analyses					1580:1587	analyses	1580:1587	analyses	1580:1587	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	3	20	theme	κ-car/SA	387:394	arg1	hydrogels					412:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	5	21	theme	hydrogel	698:705	arg1	degree					674:679	The swelling degree	661:679	The swelling degree of the composite hydrogel in NaCl solution	661:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	5	22	theme	composite	688:696	arg1	hydrogel					698:705	the composite hydrogel	684:705	the composite hydrogel in NaCl solution	684:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	10	23	theme	hydrogels	1412:1420	arg1	capacity					1381:1388	The optimal adsorption capacity	1358:1388	The optimal adsorption capacity of κ-car/SA composite hydrogels	1358:1420	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	0	24	theme	mechanical	68:77	arg1	properties					79:88	enhanced mechanical properties	59:88	enhanced mechanical properties	59:88	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	0	25	theme	enhanced	59:66	arg1	properties					79:88	enhanced mechanical properties	59:88	enhanced mechanical properties	59:88	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	11	26	theme	κ-car/SA	1706:1713	arg1	hydrogels					1725:1733	κ-car/SA composite hydrogels	1706:1733	κ-car/SA composite hydrogels	1706:1733	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	7	27	used	used	950:953	arg2	CIP					941:943	CIP	941:943	CIP	941:943	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	7	27	used	used	950:953	arg2	Ciprofloxacin					926:938	Ciprofloxacin	926:938	Ciprofloxacin (CIP)	926:944	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	7	27	used	used	950:953	arg2	pollutant					968:976	the model pollutant	958:976	the model pollutant for testing the adsorption performance	958:1015	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	11	28	theme	potential	1565:1573	arg1	test					1575:1578	the Zeta potential test	1556:1578	the Zeta potential test	1556:1578	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	0	29	theme	alginate	21:28	arg1	hydrogel					45:52	κ-Carrageenan/Sodium alginate double-network hydrogel	0:52	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.	0:129	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	8	30	theme	binding	1239:1245	arg1	sites					1247:1251	heterogeneous surface and different binding sites	1203:1251	sites	1247:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	5	31	theme	NaCl	710:713	arg1	solution					715:722	NaCl solution	710:722	NaCl solution	710:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	8	32	theme	different	1229:1237	arg1	sites					1247:1251	heterogeneous surface and different binding sites	1203:1251	sites	1247:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	10	33	theme	κ-car/SA	1393:1400	arg1	hydrogels					1412:1420	κ-car/SA composite hydrogels	1393:1420	κ-car/SA composite hydrogels	1393:1420	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	1	34	theme	offshore	206:213	arg1	areas					227:231	offshore aquaculture areas	206:231	offshore aquaculture areas	206:231	The abuse of antibiotics is becoming increasingly serious, particularly in offshore aquaculture areas.
31356999	0	35	theme	κ-Carrageenan/Sodium	0:19	arg1	hydrogel					45:52	κ-Carrageenan/Sodium alginate double-network hydrogel	0:52	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.	0:129	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	4	36	theme	increasing	618:627	arg1	concentration					629:641	the increasing concentration	614:641	the increasing concentration of κ-carrageenan	614:658	It was found that the intermolecular interaction and viscosity tend to increase with the increasing concentration of κ-carrageenan.
31356999	8	37	theme	adsorption	1112:1121	arg1	data					1132:1135	the adsorption isotherm data	1108:1135	the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites	1108:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	8	38	theme	gel	1147:1149	arg1	beads					1151:1155	gel beads	1147:1155	gel beads	1147:1155	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	12	39	theme	double-network	1769:1782	arg1	hydrogel					1784:1791	the new double-network hydrogel	1761:1791	the new double-network hydrogel	1761:1791	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	1	40	theme	aquaculture	215:225	arg1	areas					227:231	offshore aquaculture areas	206:231	offshore aquaculture areas	206:231	The abuse of antibiotics is becoming increasingly serious, particularly in offshore aquaculture areas.
31356999	10	41	theme	adsorption	1452:1461	arg1	capacity					1463:1470	the adsorption capacity	1448:1470	the adsorption capacity of the hydrogels	1448:1487	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	5	42	from	solution	715:722	arg1	degree					674:679	The swelling degree	661:679	The swelling degree of the composite hydrogel in NaCl solution	661:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	5	43	theme	carrageenan	774:784	arg1	increase					762:769	the increase	758:769	the increase of carrageenan	758:784	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	5	44	theme	swelling	665:672	arg1	degree					674:679	The swelling degree	661:679	The swelling degree of the composite hydrogel in NaCl solution	661:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	12	45	theme	new	1765:1767	arg1	hydrogel					1784:1791	the new double-network hydrogel	1761:1791	the new double-network hydrogel	1761:1791	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	8	46	from	data	1132:1135	arg1	beads					1151:1155	gel beads	1147:1155	gel beads	1147:1155	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	0	47	theme	double-network	30:43	arg1	hydrogel					45:52	κ-Carrageenan/Sodium alginate double-network hydrogel	0:52	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.	0:129	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	5	48	from	hydrogel	698:705	arg1	solution					715:722	NaCl solution	710:722	NaCl solution	710:722	The swelling degree of the composite hydrogel in NaCl solution presented a decreasing trend with the increase of carrageenan.
31356999	3	49	theme	mechanical	466:475	arg1	properties					477:486	enhanced mechanical properties	457:486	enhanced mechanical properties	457:486	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	10	50	theme	hydrogels	1479:1487	arg1	capacity					1463:1470	the adsorption capacity	1448:1470	the adsorption capacity of the hydrogels	1448:1487	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	8	51	theme	κ-car/SA	1179:1186	arg1	hydrogels					1188:1196	κ-car/SA hydrogels	1179:1196	κ-car/SA hydrogels	1179:1196	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	12	52	theme	hydrogel	1784:1791	arg1	formation					1748:1756	The formation	1744:1756	The formation of the new double-network hydrogel	1744:1791	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	3	53	theme	adsorption	508:517	arg1	capacity					519:526	adsorption capacity	508:526	adsorption capacity	508:526	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	12	54	theme	antibiotic	1866:1875	arg1	adsorption					1852:1861	the adsorption	1848:1861	the adsorption of antibiotic in water	1848:1884	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	12	55	theme	good	1802:1805	arg1	properties					1807:1816	good properties	1802:1816	good properties	1802:1816	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	2	56	theme	good	265:268	arg1	prospects					270:278	good prospects	265:278	good prospects for environmental application for the removal of antibiotics	265:339	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	0	57	with	hydrogel	45:52	arg1	capacity					121:128	adsorption capacity	110:128	adsorption capacity	110:128	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	0	57	with	hydrogel	45:52	arg1	anti-swelling					91:103	anti-swelling	91:103	anti-swelling	91:103	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	0	57	with	hydrogel	45:52	arg1	properties					79:88	enhanced mechanical properties	59:88	enhanced mechanical properties	59:88	κ-Carrageenan/Sodium alginate double-network hydrogel with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	9	58	theme	adsorption	1316:1325	arg1	properties					1327:1336	excellent adsorption properties	1306:1336	excellent adsorption properties for CIP (229 mg/g)	1306:1355	κ-car/SA composite double-network hydrogels exhibit excellent adsorption properties for CIP (229 mg/g).
31356999	10	59	theme	composite	1402:1410	arg1	hydrogels					1412:1420	κ-car/SA composite hydrogels	1393:1420	κ-car/SA composite hydrogels	1393:1420	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	11	60	theme	FTIR	1534:1537	arg1	spectroscopy					1539:1550	FTIR spectroscopy	1534:1550	FTIR spectroscopy	1534:1550	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	6	61	theme	κ-carrageenan	843:855	arg1	gel					867:869	κ-carrageenan composite gel	843:869	κ-carrageenan composite gel	843:869	SA can effectively improve the mechanical properties of κ-carrageenan composite gel and enhance its compressive resistance and elasticity.
31356999	3	62	theme	enhanced	457:464	arg1	properties					477:486	enhanced mechanical properties	457:486	enhanced mechanical properties	457:486	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	2	63	theme	polymer	249:255	arg1	gel					257:259	Double-network polymer gel	234:259	Double-network polymer gel	234:259	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	12	64	theme	potential	1834:1842	arg1	properties					1807:1816	good properties	1802:1816	good properties	1802:1816	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	8	65	theme	CIP	1140:1142	arg1	data					1132:1135	the adsorption isotherm data	1108:1135	the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites	1108:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	7	66	theme	adsorption	994:1003	arg1	performance					1005:1015	the adsorption performance	990:1015	the adsorption performance	990:1015	Ciprofloxacin (CIP) was used as the model pollutant for testing the adsorption performance.
31356999	6	67	theme	gel	867:869	arg1	properties					829:838	the mechanical properties	814:838	the mechanical properties of κ-carrageenan composite gel	814:869	SA can effectively improve the mechanical properties of κ-carrageenan composite gel and enhance its compressive resistance and elasticity.
31356999	2	68	theme	Double-network	234:247	arg1	gel					257:259	Double-network polymer gel	234:259	Double-network polymer gel	234:259	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	12	69	from	adsorption	1852:1861	arg1	water					1880:1884	water	1880:1884	water	1880:1884	The formation of the new double-network hydrogel provided good properties and development potential for the adsorption of antibiotic in water.
31356999	1	70	theme	antibiotics	144:154	arg1	abuse					135:139	The abuse	131:139	The abuse of antibiotics	131:154	The abuse of antibiotics is becoming increasingly serious, particularly in offshore aquaculture areas.
31356999	9	71	theme	composite	1263:1271	arg1	hydrogels					1288:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels exhibit excellent adsorption properties for CIP (229 mg/g).
31356999	8	72	theme	heterogeneous	1203:1215	arg1	surface					1217:1223	heterogeneous surface and different binding sites	1203:1251	surface	1217:1223	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	6	73	theme	composite	857:865	arg1	gel					867:869	κ-carrageenan composite gel	843:869	κ-carrageenan composite gel	843:869	SA can effectively improve the mechanical properties of κ-carrageenan composite gel and enhance its compressive resistance and elasticity.
31356999	6	74	theme	mechanical	818:827	arg1	properties					829:838	the mechanical properties	814:838	the mechanical properties of κ-carrageenan composite gel	814:869	SA can effectively improve the mechanical properties of κ-carrageenan composite gel and enhance its compressive resistance and elasticity.
31356999	8	75	contain	have	1198:1201	arg2	surface					1217:1223	heterogeneous surface and different binding sites	1203:1251	surface	1217:1223	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	8	75	contain	have	1198:1201	arg2	sites					1247:1251	heterogeneous surface and different binding sites	1203:1251	sites	1247:1251	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	8	75	contain	have	1198:1201	arg1	hydrogels					1188:1196	κ-car/SA hydrogels	1179:1196	κ-car/SA hydrogels	1179:1196	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	6	76	theme	compressive	887:897	arg1	resistance					899:908	its compressive resistance	883:908	its compressive resistance	883:908	SA can effectively improve the mechanical properties of κ-carrageenan composite gel and enhance its compressive resistance and elasticity.
31356999	2	77	contain	has	261:263	arg1	gel					257:259	Double-network polymer gel	234:259	Double-network polymer gel	234:259	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	2	77	contain	has	261:263	arg2	prospects					270:278	good prospects	265:278	good prospects for environmental application for the removal of antibiotics	265:339	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	3	78	theme	Alginate	377:384	arg1	hydrogels					412:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels	356:420	In this work, κ-Carrageenan/Sodium Alginate (κ-car/SA) double-network hydrogels were designed and synthesized with enhanced mechanical properties, anti-swelling, and adsorption capacity.
31356999	10	79	theme	optimal	1362:1368	arg1	capacity					1381:1388	The optimal adsorption capacity	1358:1388	The optimal adsorption capacity of κ-car/SA composite hydrogels	1358:1420	The optimal adsorption capacity of κ-car/SA composite hydrogels was obtained at pH 4, and the adsorption capacity of the hydrogels increased with increasing ion concentration.
31356999	2	80	theme	environmental	284:296	arg1	application					298:308	environmental application	284:308	environmental application	284:308	Double-network polymer gel has good prospects for environmental application for the removal of antibiotics.
31356999	9	81	theme	κ-car/SA	1254:1261	arg1	hydrogels					1288:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels	1254:1296	κ-car/SA composite double-network hydrogels exhibit excellent adsorption properties for CIP (229 mg/g).
31356999	11	82	theme	adsorption	1605:1614	arg1	mechanism					1616:1624	the adsorption mechanism	1601:1624	the adsorption mechanism	1601:1624	FTIR spectroscopy and the Zeta potential test analyses showed that the adsorption mechanism may be explained by hydrogen bonding and the electrostatic interactions between κ-car/SA composite hydrogels and CIP.
31356999	8	83	theme	isotherm	1064:1071	arg1	model					1073:1077	the Langmuir-Freundlich isotherm model	1040:1077	the Langmuir-Freundlich isotherm model	1040:1077	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
31356999	8	83	theme	isotherm	1064:1071	arg1	suitable					1087:1094	suitable	1087:1094	suitable	1087:1094	The results show that the Langmuir-Freundlich isotherm model is more suitable for fitting the adsorption isotherm data of CIP on gel beads, which indicates that κ-car/SA hydrogels have heterogeneous surface and different binding sites.
30295917	11	0	theme	property	1974:1981	arg1	preservation					1983:1994	fresh-keeping property preservation	1960:1994	fresh-keeping property preservation	1960:1994	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	11	1	theme	great	2006:2010	arg1	potential					2024:2032	great application potential	2006:2032	great application potential	2006:2032	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	8	2	theme	antibacterial	1416:1428	arg1	activity					1430:1437	antibacterial activity	1416:1437	antibacterial activity	1416:1437	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	8	3	theme	physical	1394:1401	arg1	property					1403:1410	physical property	1394:1410	physical property	1394:1410	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	9	4	dep	films	1489:1493	arg1	acting					1496:1501	acting	1496:1501	acting as suitable carriers for L. paracasei	1496:1539	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	4	dep	films	1489:1493	arg1	possessing					1545:1554	possessing	1545:1554	possessing noteworthy bacteriostatic activities	1545:1591	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	3	5	theme	scanning	511:518	arg1	microscopy					529:538	scanning electron microscopy	511:538	scanning electron microscopy	511:538	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	10	6	theme	PRACTICAL	1657:1665	arg1	APPLICATION					1667:1677	PRACTICAL APPLICATION	1657:1677	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging	1657:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	6	7	theme	oxygen	977:982	arg1	permeability					984:995	oxygen permeability	977:995	oxygen permeability	977:995	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	11	8	theme	application	2012:2022	arg1	potential					2024:2032	great application potential	2006:2032	great application potential	2006:2032	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	2	9	theme	mechanical	303:312	arg1	optical					315:321	The mechanical, optical, and barrier properties	299:345	optical	315:321	The mechanical, optical, and barrier properties were tested to determine the influence of the addition of lactobacilli into complex films.
30295917	0	10	theme	Preservation	116:127	arg1	Effect					129:134	Preservation Effect	116:134	Preservation Effect	116:134	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	8	11	dep	gram-positive	1235:1247	arg1	aureus					1289:1294	Staphylococcus aureus	1274:1294	Staphylococcus aureus	1274:1294	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	8	11	dep	gram-positive	1235:1247	arg1	coli					1328:1331	Escherichia coli	1316:1331	Escherichia coli	1316:1331	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	8	11	dep	gram-positive	1235:1247	arg1	typhimurium					1345:1355	Salmonella typhimurium	1334:1355	Salmonella typhimurium	1334:1355	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	8	11	dep	gram-positive	1235:1247	arg1	monocytogenes					1259:1271	Listeria monocytogenes	1250:1271	Listeria monocytogenes	1250:1271	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	11	12	contain	containing	1867:1876	arg1	films					1861:1865	the prepared films	1848:1865	the prepared films containing lactic acid bacteria	1848:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	11	12	contain	containing	1867:1876	arg2	bacteria					1890:1897	lactic acid bacteria	1878:1897	lactic acid bacteria	1878:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	9	13	theme	bioactive	1616:1624	arg1	films					1489:1493	The composite films	1475:1493	The composite films	1475:1493	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	13	theme	bioactive	1616:1624	arg1	packaging					1626:1634	bioactive packaging	1616:1634	bioactive packaging for preserving food	1616:1654	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	1	14	theme	casting	283:289	arg1	method					291:296	a casting method	281:296	a casting method	281:296	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	5	15	theme	lactobacilli	786:797	arg1	activities					821:830	the antibacterial activities	803:830	the antibacterial activities of the bioactive films against pathogenic organisms	803:882	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	5	15	theme	lactobacilli	786:797	arg1	viability					769:777	the microbial viability	755:777	the microbial viability of the lactobacilli	755:797	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	3	16	theme	composite	494:502	arg1	films					504:508	the composite films	490:508	the composite films	490:508	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	11	17	contain	have	2001:2004	arg1	films					1861:1865	the prepared films	1848:1865	the prepared films containing lactic acid bacteria	1848:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	11	17	contain	have	2001:2004	arg2	potential					2024:2032	great application potential	2006:2032	great application potential	2006:2032	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	2	18	theme	complex	423:429	arg1	films					431:435	complex films	423:435	complex films	423:435	The mechanical, optical, and barrier properties were tested to determine the influence of the addition of lactobacilli into complex films.
30295917	8	19	theme	storage	1448:1454	arg1	period					1456:1461	a storage period	1446:1461	a storage period of 30 days	1446:1472	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	6	20	theme	L.	1158:1159	arg1	paracase					1161:1168	L. paracase	1158:1168	L. paracase	1158:1168	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	8	21	theme	Listeria	1250:1257	arg1	aureus					1289:1294	Staphylococcus aureus	1274:1294	Staphylococcus aureus	1274:1294	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	8	21	theme	Listeria	1250:1257	arg1	monocytogenes					1259:1271	Listeria monocytogenes	1250:1271	Listeria monocytogenes	1250:1271	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	6	22	theme	crystalline	953:963	arg1	properties					965:974	crystalline properties	953:974	crystalline properties	953:974	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	8	23	theme	gram-negative	1301:1313	arg1	pathogens					1358:1366	both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens	1230:1366	both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens	1230:1366	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	3	24	theme	atomic	544:549	arg1	microscopy					557:566	atomic force microscopy	544:566	atomic force microscopy	544:566	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	6	25	theme	paracase	1161:1168	arg1	incorporation					1141:1153	the incorporation	1137:1153	the incorporation of L. paracase	1137:1168	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	2	26	theme	addition	393:400	arg1	influence					376:384	the influence	372:384	the influence of the addition of lactobacilli into complex films	372:435	The mechanical, optical, and barrier properties were tested to determine the influence of the addition of lactobacilli into complex films.
30295917	5	27	theme	pathogenic	863:872	arg1	organisms					874:882	pathogenic organisms	863:882	pathogenic organisms	863:882	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	11	28	theme	acid	1885:1888	arg1	bacteria					1890:1897	lactic acid bacteria	1878:1897	lactic acid bacteria	1878:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	11	29	theme	antipathogenic	1931:1944	arg1	activity					1946:1953	antipathogenic activity	1931:1953	antipathogenic activity	1931:1953	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	1	30	theme	hydroxypropyl	218:230	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	10	31	theme	desires	1704:1710	arg1	sake					1687:1690	the sake	1683:1690	the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging	1683:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	10	32	theme	innovative	1773:1782	arg1	packaging					1794:1802	innovative bioactive packaging	1773:1802	innovative bioactive packaging	1773:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	11	33	theme	physical	1912:1919	arg1	property					1921:1928	great physical property	1906:1928	great physical property	1906:1928	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	1	34	theme	cellulose	232:240	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	9	35	theme	bacteriostatic	1567:1580	arg1	activities					1582:1591	noteworthy bacteriostatic activities	1556:1591	noteworthy bacteriostatic activities	1556:1591	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	35	theme	bacteriostatic	1567:1580	arg1	carriers					1515:1522	suitable carriers	1506:1522	suitable carriers for L. paracasei	1506:1539	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	10	36	theme	high	1699:1702	arg1	desires					1704:1710	the high desires	1695:1710	the high desires of consumers for food safety and quality, the development of innovative bioactive packaging	1695:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	11	37	theme	prepared	1852:1859	arg1	films					1861:1865	the prepared films	1848:1865	the prepared films containing lactic acid bacteria	1848:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	8	38	theme	days	1469:1472	arg1	period					1456:1461	a storage period	1446:1461	a storage period of 30 days	1446:1472	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	3	39	theme	surface	468:474	arg1	morphology					476:485	the surface morphology	464:485	the surface morphology of the composite films	464:508	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	11	40	theme	lactic	1878:1883	arg1	bacteria					1890:1897	lactic acid bacteria	1878:1897	lactic acid bacteria	1878:1897	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	1	41	theme	Bioactive	137:145	arg1	films					157:161	Bioactive composite films	137:161	Bioactive composite films	137:161	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	9	42	theme	suitable	1506:1513	arg1	carriers					1515:1522	suitable carriers	1506:1522	suitable carriers for L. paracasei	1506:1539	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	42	theme	suitable	1506:1513	arg1	activities					1582:1591	noteworthy bacteriostatic activities	1556:1591	noteworthy bacteriostatic activities	1556:1591	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	1	43	theme	HPC	243:245	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	0	44	theme	Composite	56:64	arg1	Films					66:70	Lactobacilli-Loaded Composite Films	36:70	Lactobacilli-Loaded Composite Films	36:70	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	10	45	theme	packaging	1794:1802	arg1	development					1758:1768	the development	1754:1768	the development of innovative bioactive packaging	1754:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	10	45	theme	packaging	1794:1802	arg1	safety					1734:1739	food safety	1729:1739	food safety	1729:1739	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	5	46	theme	microbial	759:767	arg1	viability					769:777	the microbial viability	755:777	the microbial viability of the lactobacilli	755:797	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	6	47	theme	mechanical	930:939	arg1	properties					941:950	the mechanical properties	926:950	the mechanical properties	926:950	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	10	48	theme	food	1729:1732	arg1	development					1758:1768	the development	1754:1768	the development of innovative bioactive packaging	1754:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	10	48	theme	food	1729:1732	arg1	safety					1734:1739	food safety	1729:1739	food safety	1729:1739	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	9	49	theme	noteworthy	1556:1565	arg1	activities					1582:1591	noteworthy bacteriostatic activities	1556:1591	noteworthy bacteriostatic activities	1556:1591	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	49	theme	noteworthy	1556:1565	arg1	carriers					1515:1522	suitable carriers	1506:1522	suitable carriers for L. paracasei	1506:1539	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	0	50	theme	Lactobacilli-Loaded	36:54	arg1	Films					66:70	Lactobacilli-Loaded Composite Films	36:70	Lactobacilli-Loaded Composite Films	36:70	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	5	51	theme	bioactive	839:847	arg1	films					849:853	the bioactive films	835:853	the bioactive films against pathogenic organisms	835:882	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	4	52	dep	transform	594:602	arg1	infrared					604:611	infrared	604:611	transform infrared spectroscopy and X-ray diffraction analyses	594:655	Fourier transform infrared spectroscopy and X-ray diffraction analyses were conducted to evaluate intermolecular interactions and crystallinity, respectively.
30295917	6	53	theme	vapor	1084:1088	arg1	properties					1098:1107	the gloss and water vapor barrier properties	1064:1107	properties	1098:1107	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	1	54	theme	-konjac	247:253	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	5	55	theme	films	849:853	arg1	activities					821:830	the antibacterial activities	803:830	the antibacterial activities of the bioactive films against pathogenic organisms	803:882	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	5	55	theme	films	849:853	arg1	viability					769:777	the microbial viability	755:777	the microbial viability of the lactobacilli	755:797	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	8	56	theme	paracasei	1185:1193	arg1	films					1195:1199	paracasei films	1185:1199	paracasei films	1185:1199	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	4	57	dep	Fourier	586:592	arg1	transform					594:602	transform	594:602	transform infrared spectroscopy and X-ray diffraction analyses	594:655	Fourier transform infrared spectroscopy and X-ray diffraction analyses were conducted to evaluate intermolecular interactions and crystallinity, respectively.
30295917	1	58	theme	composite	147:155	arg1	films					157:161	Bioactive composite films	137:161	Bioactive composite films	137:161	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	0	59	theme	Films	66:70	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	0	59	theme	Films	66:70	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	11	60	theme	great	1906:1910	arg1	property					1921:1928	great physical property	1906:1928	great physical property	1906:1928	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	3	61	theme	films	504:508	arg1	morphology					476:485	the surface morphology	464:485	the surface morphology of the composite films	464:508	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	6	62	theme	color	1002:1006	arg1	characteristics					1008:1022	color characteristics	1002:1022	color characteristics	1002:1022	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	10	63	theme	wide	1818:1821	arg1	attention					1823:1831	wide attention	1818:1831	wide attention	1818:1831	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	8	64	theme	gram-positive	1235:1247	arg1	pathogens					1358:1366	both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens	1230:1366	both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens	1230:1366	paracasei films were effective in inhibiting both gram-positive (Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Salmonella typhimurium) pathogens, and the films can retain physical property and antibacterial activity within a storage period of 30 days.
30295917	11	65	theme	fresh-keeping	1960:1972	arg1	preservation					1983:1994	fresh-keeping property preservation	1960:1994	fresh-keeping property preservation	1960:1994	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	10	66	theme	bioactive	1784:1792	arg1	packaging					1794:1802	innovative bioactive packaging	1773:1802	innovative bioactive packaging	1773:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	1	67	theme	flour	255:259	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	3	68	theme	force	551:555	arg1	microscopy					557:566	atomic force microscopy	544:566	atomic force microscopy	544:566	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	9	69	theme	composite	1479:1487	arg1	films					1489:1493	The composite films	1475:1493	The composite films	1475:1493	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	9	69	theme	composite	1479:1487	arg1	packaging					1626:1634	bioactive packaging	1616:1634	bioactive packaging for preserving food	1616:1654	The composite films, acting as suitable carriers for L. paracasei and possessing noteworthy bacteriostatic activities, could be developed as bioactive packaging for preserving food.
30295917	2	70	theme	barrier	328:334	arg1	properties					336:345	The mechanical, optical, and barrier properties	299:345	properties	336:345	The mechanical, optical, and barrier properties were tested to determine the influence of the addition of lactobacilli into complex films.
30295917	6	71	theme	barrier	1090:1096	arg1	properties					1098:1107	the gloss and water vapor barrier properties	1064:1107	properties	1098:1107	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	0	72	theme	Antipathogenic	88:101	arg1	Activity					103:110	Antipathogenic Activity	88:110	Antipathogenic Activity	88:110	Preparation and Characterization of Lactobacilli-Loaded Composite Films with Sustaining Antipathogenic Activity and Preservation Effect.
30295917	2	73	theme	lactobacilli	405:416	arg1	addition					393:400	the addition	389:400	the addition of lactobacilli	389:416	The mechanical, optical, and barrier properties were tested to determine the influence of the addition of lactobacilli into complex films.
30295917	11	74	theme	fresh	2037:2041	arg1	preservation					2048:2059	fresh food preservation	2037:2059	fresh food preservation	2037:2059	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	3	75	theme	electron	520:527	arg1	microscopy					529:538	scanning electron microscopy	511:538	scanning electron microscopy	511:538	For purpose of evaluating the surface morphology of the composite films, scanning electron microscopy and atomic force microscopy were carried out.
30295917	4	76	theme	intermolecular	684:697	arg1	interactions					699:710	intermolecular interactions	684:710	intermolecular interactions	684:710	Fourier transform infrared spectroscopy and X-ray diffraction analyses were conducted to evaluate intermolecular interactions and crystallinity, respectively.
30295917	1	77	theme	KF	262:263	arg1	matrix					266:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix	216:271	Bioactive composite films were obtained by adding Lactobacillus paracasei into a hydroxypropyl cellulose (HPC)-konjac flour (KF) matrix through a casting method.
30295917	5	78	theme	antibacterial	807:819	arg1	activities					821:830	the antibacterial activities	803:830	the antibacterial activities of the bioactive films against pathogenic organisms	803:882	Moreover, the microbial viability of the lactobacilli and the antibacterial activities of the bioactive films against pathogenic organisms were measured.
30295917	11	79	theme	food	2043:2046	arg1	preservation					2048:2059	fresh food preservation	2037:2059	fresh food preservation	2037:2059	In this work, the prepared films containing lactic acid bacteria showed great physical property, antipathogenic activity, and fresh-keeping property preservation, and have great application potential in fresh food preservation.
30295917	6	80	theme	water	1078:1082	arg1	properties					1098:1107	the gloss and water vapor barrier properties	1064:1107	properties	1098:1107	The results indicated that the mechanical properties, crystalline properties, oxygen permeability, and color characteristics were not notably altered; nevertheless, the gloss and water vapor barrier properties were relatively weakened by the incorporation of L. paracase.
30295917	4	81	theme	X-ray	630:634	arg1	analyses					648:655	X-ray diffraction analyses	630:655	X-ray diffraction analyses	630:655	Fourier transform infrared spectroscopy and X-ray diffraction analyses were conducted to evaluate intermolecular interactions and crystallinity, respectively.
30295917	10	82	theme	consumers	1715:1723	arg1	desires					1704:1710	the high desires	1695:1710	the high desires of consumers for food safety and quality, the development of innovative bioactive packaging	1695:1802	PRACTICAL APPLICATION For the sake of the high desires of consumers for food safety and quality, the development of innovative bioactive packaging has attracted wide attention.
30295917	4	83	theme	diffraction	636:646	arg1	analyses					648:655	X-ray diffraction analyses	630:655	X-ray diffraction analyses	630:655	Fourier transform infrared spectroscopy and X-ray diffraction analyses were conducted to evaluate intermolecular interactions and crystallinity, respectively.
31689898	2	0	from	concentration	366:378	arg1	relation					383:390	relation	383:390	relation to maternal HMOs	383:407	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	10	1	from	HMOs	1294:1297	arg1	blood					1307:1311	cord blood	1302:1311	cord blood	1302:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	10	2	theme	HMOs	1294:1297	arg1	evidence					1282:1289	direct evidence	1275:1289	direct evidence of HMOs in cord blood	1275:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	0	3	from	Oligosaccharides	23:38	arg1	Blood					48:52	Cord Blood	43:52	Cord Blood	43:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	2	4	theme	HMO	346:348	arg1	composition					350:360	HMO composition	346:360	HMO composition	346:360	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	11	5	theme	specific	1468:1475	arg1	HMOs					1477:1480	specific HMOs	1468:1480	specific HMOs	1468:1480	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	7	6	theme	maternal	1011:1018	arg1	serum					1020:1024	maternal serum	1011:1024	maternal serum	1011:1024	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	1	7	from	present	146:152	arg1	serum					166:170	maternal serum	157:170	maternal serum in early gestation	157:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	7	8	theme	blood	946:950	arg1	concentration					956:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	10	9	from	blood	1307:1311	arg1	evidence					1282:1289	direct evidence	1275:1289	direct evidence of HMOs in cord blood	1275:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	0	10	from	Evidence	0:7	arg1	Blood					48:52	Cord Blood	43:52	Cord Blood	43:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	11	11	theme	HMOs	1477:1480	arg1	transfer					1456:1463	the transfer	1452:1463	the transfer of specific HMOs	1452:1480	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	1	12	from	serum	166:170	arg1	present					146:152	present	146:152	present	146:152	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	12	from	serum	166:170	arg1	gestation					181:189	early gestation	175:189	early gestation	175:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	6	13	theme	maternal	873:880	arg1	serum					882:886	maternal serum	873:886	maternal serum	873:886	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	0	14	from	Blood	48:52	arg1	Transport					76:84	Maternal-to-Fetal Transport	58:84	Maternal-to-Fetal Transport across the Placenta	58:104	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	0	14	from	Blood	48:52	arg1	Evidence					0:7	Evidence	0:7	Evidence of Human Milk Oligosaccharides in Cord Blood	0:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	10	15	theme	direct	1275:1280	arg1	evidence					1282:1289	direct evidence	1275:1289	direct evidence of HMOs in cord blood	1275:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	1	16	theme	placental	243:251	arg1	barrier					253:259	the placental barrier	239:259	the placental barrier	239:259	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	4	17	theme	=	636:636	arg1	pregnancies					621:631	normal pregnancies	614:631	normal pregnancies (n = 22)	614:640	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	4	17	theme	=	636:636	arg1	n					634:634	n = 22	634:639	n = 22	634:639	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	4	18	theme	matching	552:559	arg1	blood					573:577	matching venous cord blood	552:577	matching venous cord blood	552:577	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	1	19	theme	maternal	157:164	arg1	serum					166:170	maternal serum	157:170	maternal serum in early gestation	157:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	5	20	theme	maternal-to-fetal	658:674	arg1	transport					676:684	maternal-to-fetal transport	658:684	maternal-to-fetal transport	658:684	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	5	21	theme	=	754:754	arg1	pregnancies					739:749	term pregnancies	734:749	term pregnancies (n = 3) with 2'-fucosyllactose (2'FL)	734:787	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	5	21	theme	=	754:754	arg1	n					752:752	n = 3	752:756	n = 3	752:756	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	11	22	theme	Future	1395:1400	arg1	studies					1402:1408	Future studies	1395:1408	Future studies	1395:1408	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	8	23	theme	maternal	1072:1079	arg1	serum					1081:1085	maternal serum	1072:1085	maternal serum	1072:1085	HMO composition resembled the composition in maternal serum, with the strongest correlations for 2'FL and LDFT.
31689898	10	24	theme	fetal	1380:1384	arg1	circuit					1386:1392	fetal circuit	1380:1392	fetal circuit	1380:1392	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	5	25	theme	term	734:737	arg1	pregnancies					739:749	term pregnancies	734:749	term pregnancies (n = 3) with 2'-fucosyllactose (2'FL)	734:787	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	5	25	theme	term	734:737	arg1	n					752:752	n = 3	752:756	n = 3	752:756	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	1	26	attach	present	146:152	arg2	HMOs					136:139	HMOs	136:139	HMOs	136:139	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	26	attach	present	146:152	arg1	serum					166:170	maternal serum	157:170	maternal serum in early gestation	157:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	26	attach	present	146:152	arg2	oligosaccharides					118:133	Human milk oligosaccharides	107:133	Human milk oligosaccharides (HMOs)	107:140	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	10	27	theme	cord	1302:1305	arg1	blood					1307:1311	cord blood	1302:1311	cord blood	1302:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	2	28	from	HMOs	315:318	arg1	blood					328:332	cord blood	323:332	cord blood	323:332	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	7	29	theme	total	935:939	arg1	concentration					956:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	2	30	theme	maternal	395:402	arg1	HMOs					404:407	maternal HMOs	395:407	maternal HMOs	395:407	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	6	31	theme	present	862:868	arg1	oligosaccharides					835:850	up to 18 oligosaccharides	826:850	up to 18 oligosaccharides typically present in maternal serum	826:886	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	6	32	from	serum	882:886	arg1	present					862:868	present	862:868	present	862:868	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	7	33	from	concentration	994:1006	arg1	serum					1020:1024	maternal serum	1011:1024	maternal serum	1011:1024	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	1	34	theme	early	175:179	arg1	gestation					181:189	early gestation	175:189	early gestation	175:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	0	35	theme	Milk	18:21	arg1	Oligosaccharides					23:38	Human Milk Oligosaccharides	12:38	Human Milk Oligosaccharides in Cord Blood	12:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	9	36	theme	offered	1191:1197	arg1	FL					1201:1202	maternally offered 2'FL	1180:1202	maternally offered 2'FL	1180:1202	After 180 min perfusion, we found 22% of maternally offered 2'FL in the fetal circuit without reaching equilibrium.
31689898	5	37	with	pregnancies	739:749	arg1	FL					785:786	2'FL	783:786	2'FL	783:786	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	5	37	with	pregnancies	739:749	arg1	2'-fucosyllactose					764:780	2'-fucosyllactose	764:780	2'-fucosyllactose (2'FL)	764:787	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	4	38	theme	venous	561:566	arg1	blood					573:577	matching venous cord blood	552:577	matching venous cord blood	552:577	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	0	39	theme	Human	12:16	arg1	Oligosaccharides					23:38	Human Milk Oligosaccharides	12:38	Human Milk Oligosaccharides in Cord Blood	12:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	7	40	theme	cord	941:944	arg1	concentration					956:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	5	41	dep	perfused	690:697	arg1	isolated					699:706	isolated	699:706	perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting	690:814	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	1	42	theme	Human	107:111	arg1	HMOs					136:139	HMOs	136:139	HMOs	136:139	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	42	theme	Human	107:111	arg1	oligosaccharides					118:133	Human milk oligosaccharides	107:133	Human milk oligosaccharides (HMOs)	107:140	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	3	43	theme	ex-vivo	416:422	arg1	model					444:448	an ex-vivo placental perfusion model	413:448	an ex-vivo placental perfusion model	413:448	In an ex-vivo placental perfusion model, we asked whether HMOs can pass over the placenta.
31689898	8	44	theme	strongest	1097:1105	arg1	correlations					1107:1118	the strongest correlations	1093:1118	the strongest correlations for 2'FL and LDFT	1093:1136	HMO composition resembled the composition in maternal serum, with the strongest correlations for 2'FL and LDFT.
31689898	1	45	theme	fetal	271:275	arg1	circulation					277:287	fetal circulation	271:287	fetal circulation	271:287	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	6	46	from	present	862:868	arg1	serum					882:886	maternal serum	873:886	maternal serum	873:886	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	0	47	from	Transport	76:84	arg1	Blood					48:52	Cord Blood	43:52	Cord Blood	43:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	4	48	theme	blood	573:577	arg1	samples					579:585	maternal serum and matching venous cord blood samples	533:585	maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22)	533:640	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	0	49	theme	Oligosaccharides	23:38	arg1	Transport					76:84	Maternal-to-Fetal Transport	58:84	Maternal-to-Fetal Transport across the Placenta	58:104	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	0	49	theme	Oligosaccharides	23:38	arg1	Evidence					0:7	Evidence	0:7	Evidence of Human Milk Oligosaccharides in Cord Blood	0:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	5	50	theme	placental	708:716	arg1	cotyledons					718:727	placental cotyledons	708:727	placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL)	708:787	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	8	51	theme	HMO	1027:1029	arg1	composition					1031:1041	HMO composition	1027:1041	HMO composition	1027:1041	HMO composition resembled the composition in maternal serum, with the strongest correlations for 2'FL and LDFT.
31689898	11	52	theme	potential	1427:1435	arg1	differences					1437:1447	potential differences	1427:1447	potential differences in the transfer of specific HMOs, or in pregnancy disorders	1427:1507	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	4	53	theme	cord	568:571	arg1	blood					573:577	matching venous cord blood	552:577	matching venous cord blood	552:577	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	7	54	theme	HMO	952:954	arg1	concentration					956:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	7	55	theme	Median	928:933	arg1	concentration					956:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration	928:968	Median total cord blood HMO concentration did not differ from the concentration in maternal serum.
31689898	11	56	theme	pregnancy	1489:1497	arg1	disorders					1499:1507	pregnancy disorders	1489:1507	pregnancy disorders	1489:1507	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	10	57	from	evidence	1282:1289	arg1	blood					1307:1311	cord blood	1302:1311	cord blood	1302:1311	Our results provide direct evidence of HMOs in cord blood, and suggest that the placenta transfers HMOs from the maternal to fetal circuit.
31689898	1	58	theme	milk	113:116	arg1	HMOs					136:139	HMOs	136:139	HMOs	136:139	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	58	theme	milk	113:116	arg1	oligosaccharides					118:133	Human milk oligosaccharides	107:133	Human milk oligosaccharides (HMOs)	107:140	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	0	59	theme	Cord	43:46	arg1	Blood					48:52	Cord Blood	43:52	Cord Blood	43:52	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	1	60	located	present	146:152	arg2	HMOs					136:139	HMOs	136:139	HMOs	136:139	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	60	located	present	146:152	arg1	serum					166:170	maternal serum	157:170	maternal serum in early gestation	157:189	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	1	60	located	present	146:152	arg2	oligosaccharides					118:133	Human milk oligosaccharides	107:133	Human milk oligosaccharides (HMOs)	107:140	Human milk oligosaccharides (HMOs) are present in maternal serum in early gestation, raising the question of whether HMOs can cross the placental barrier and reach fetal circulation.
31689898	8	61	from	composition	1057:1067	arg1	serum					1081:1085	maternal serum	1072:1085	maternal serum	1072:1085	HMO composition resembled the composition in maternal serum, with the strongest correlations for 2'FL and LDFT.
31689898	5	62	from	pregnancies	739:749	arg1	cotyledons					718:727	placental cotyledons	708:727	placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL)	708:787	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	5	63	theme	closed	801:806	arg1	setting					808:814	a double closed setting	792:814	a double closed setting	792:814	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	9	64	theme	fetal	1211:1215	arg1	circuit					1217:1223	the fetal circuit	1207:1223	the fetal circuit	1207:1223	After 180 min perfusion, we found 22% of maternally offered 2'FL in the fetal circuit without reaching equilibrium.
31689898	6	65	dep	18	832:833	arg1	to					829:830	to	829:830	to	829:830	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	3	66	theme	placental	424:432	arg1	model					444:448	an ex-vivo placental perfusion model	413:448	an ex-vivo placental perfusion model	413:448	In an ex-vivo placental perfusion model, we asked whether HMOs can pass over the placenta.
31689898	4	67	theme	maternal	533:540	arg1	serum					542:546	maternal serum	533:546	maternal serum	533:546	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	5	68	theme	double	794:799	arg1	setting					808:814	a double closed setting	792:814	a double closed setting	792:814	To investigate maternal-to-fetal transport, we perfused isolated placental cotyledons from term pregnancies (n = 3) with 2'-fucosyllactose (2'FL) in a double closed setting.
31689898	6	69	attach	present	862:868	arg1	serum					882:886	maternal serum	873:886	maternal serum	873:886	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	6	69	attach	present	862:868	arg2	oligosaccharides					835:850	up to 18 oligosaccharides	826:850	up to 18 oligosaccharides typically present in maternal serum	826:886	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	3	70	theme	perfusion	434:442	arg1	model					444:448	an ex-vivo placental perfusion model	413:448	an ex-vivo placental perfusion model	413:448	In an ex-vivo placental perfusion model, we asked whether HMOs can pass over the placenta.
31689898	6	71	theme	serum	900:904	arg1	samples					906:912	all cord serum samples	891:912	all cord serum samples investigated	891:925	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	0	72	theme	Maternal-to-Fetal	58:74	arg1	Transport					76:84	Maternal-to-Fetal Transport	58:84	Maternal-to-Fetal Transport across the Placenta	58:104	Evidence of Human Milk Oligosaccharides in Cord Blood and Maternal-to-Fetal Transport across the Placenta.
31689898	9	73	theme	180	1145:1147	arg1	min					1149:1151	min	1149:1151	min	1149:1151	After 180 min perfusion, we found 22% of maternally offered 2'FL in the fetal circuit without reaching equilibrium.
31689898	2	74	from	composition	350:360	arg1	relation					383:390	relation	383:390	relation to maternal HMOs	383:407	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	4	75	theme	normal	614:619	arg1	pregnancies					621:631	normal pregnancies	614:631	normal pregnancies (n = 22)	614:640	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	4	75	theme	normal	614:619	arg1	n					634:634	n = 22	634:639	n = 22	634:639	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
31689898	6	76	theme	cord	895:898	arg1	samples					906:912	all cord serum samples	891:912	all cord serum samples investigated	891:925	We found up to 18 oligosaccharides typically present in maternal serum in all cord serum samples investigated.
31689898	9	77	theme	min	1149:1151	arg1	perfusion					1153:1161	180 min perfusion	1145:1161	180 min perfusion	1145:1161	After 180 min perfusion, we found 22% of maternally offered 2'FL in the fetal circuit without reaching equilibrium.
31689898	2	78	theme	cord	323:326	arg1	blood					328:332	cord blood	323:332	cord blood	323:332	Here, we aimed to detect HMOs in cord blood, and assess HMO composition and concentration in relation to maternal HMOs.
31689898	11	79	from	differences	1437:1447	arg1	transfer					1456:1463	the transfer	1452:1463	the transfer of specific HMOs	1452:1480	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	11	79	from	differences	1437:1447	arg1	disorders					1499:1507	pregnancy disorders	1489:1507	pregnancy disorders	1489:1507	Future studies will investigate potential differences in the transfer of specific HMOs, or in pregnancy disorders.
31689898	4	80	theme	serum	542:546	arg1	samples					579:585	maternal serum and matching venous cord blood samples	533:585	maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22)	533:640	Using HPLC, we measured HMOs in maternal serum and matching venous cord blood samples collected at delivery from normal pregnancies (n = 22).
30553856	3	0	theme	TCA	283:285	arg1	deproteinization					263:278	deproteinization	263:278	deproteinization	263:278	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	3	0	theme	TCA	283:285	arg1	extraction					225:234	the hot water extraction	211:234	the hot water extraction	211:234	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	3	0	theme	TCA	283:285	arg1	precipitation					245:257	ethanol precipitation	237:257	ethanol precipitation	237:257	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	2	1	dep	Johnst	163:168	arg1	polysaccharide					118:131	A polysaccharide	116:131	A polysaccharide from Arnebia euchroma (Royle)	116:161	A polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	11	2	theme	effect	1205:1210	arg1	experiments					1186:1196	The experiments	1182:1196	The experiments of the effect of ARP on the activation of macrophage in vitro	1182:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	7	3	dep	in	778:779	arg1	vitro					781:785	vitro	781:785	vitro	781:785	The in vitro experiment indicated that ARP enhanced B- and T-lymphocyte proliferation.
30553856	0	4	from	Johnst	101:106	arg1	Characterizations					0:16	Characterizations	0:16	Characterizations	0:16	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	0	4	from	Johnst	101:106	arg1	activities					40:49	immunostimulatory activities	22:49	immunostimulatory activities	22:49	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	0	4	from	Johnst	101:106	arg1	polysaccharide					56:69	a polysaccharide	54:69	a polysaccharide from Arnebia euchroma (Royle) Johnst	54:106	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	5	5	theme	molar	547:551	arg1	ratio					553:557	a molar ratio	545:557	a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2	545:587	Monosaccharide composition analysis revealed that ARP was composed of Gal, Ara, Glu, Man, Rha and Fuc at a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2.
30553856	6	6	theme	Methylation	590:600	arg1	analysis					602:609	Methylation analysis	590:609	Methylation analysis	590:609	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	0	7	from	Characterizations	0:16	arg1	Johnst					101:106	Johnst	101:106	Johnst	101:106	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	3	8	theme	named	171:175	arg1	ARP					177:179	named ARP	171:179	named ARP	171:179	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	10	9	contain	had	1128:1130	arg1	ARP					1124:1126	ARP	1124:1126	ARP	1124:1126	Meanwhile, ARP had little effect on interleukin-2 (IL-2) production.
30553856	10	9	contain	had	1128:1130	arg2	effect					1139:1144	little effect	1132:1144	little effect	1132:1144	Meanwhile, ARP had little effect on interleukin-2 (IL-2) production.
30553856	10	9	contain	had	1128:1130	arg1	Meanwhile					1113:1121	Meanwhile	1113:1121	Meanwhile	1113:1121	Meanwhile, ARP had little effect on interleukin-2 (IL-2) production.
30553856	11	10	from	experiments	1186:1196	arg1	activation					1226:1235	the activation	1222:1235	the activation of macrophage in vitro	1222:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	6	11	theme	Ara	736:738	arg1	1,3‑linkages					760:771	1,3‑linkages	760:771	1,3‑linkages	760:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	11	theme	Ara	736:738	arg1	1,5‑					752:755	1,5‑	752:755	1,5‑	752:755	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	11	theme	Ara	736:738	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	4	12	theme	calibration	389:399	arg1	curve					401:405	a calibration curve	387:405	a calibration curve obtained with dextran standards	387:437	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	7	13	dep	B-	826:827	arg1	proliferation					846:858	proliferation	846:858	proliferation	846:858	The in vitro experiment indicated that ARP enhanced B- and T-lymphocyte proliferation.
30553856	11	14	theme	functional	1389:1398	arg1	activation					1400:1409	the functional activation	1385:1409	the functional activation of macrophage	1385:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	5	15	theme	53.8:21.3:11.7:6.8:4.3:2.2	562:587	arg1	ratio					553:557	a molar ratio	545:557	a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2	545:587	Monosaccharide composition analysis revealed that ARP was composed of Gal, Ara, Glu, Man, Rha and Fuc at a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2.
30553856	6	16	theme	1,3‑linkages	760:771	arg1	1,3‑linkages					760:771	1,3‑linkages	760:771	1,3‑linkages	760:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	16	theme	1,3‑linkages	760:771	arg1	1,5‑					752:755	1,5‑	752:755	1,5‑	752:755	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	16	theme	1,3‑linkages	760:771	arg1	1,6‑linkages					719:730	1,6‑linkages	719:730	1,6‑linkages	719:730	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	16	theme	1,3‑linkages	760:771	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	8	17	theme	dose-dependent	863:876	arg1	relationship					878:889	A dose-dependent relationship	861:889	A dose-dependent relationship	861:889	A dose-dependent relationship was observed, and a dose of 200 μg/mL resulted in the highest cell viability.
30553856	11	18	theme	IL-6	1327:1330	arg1	production					1306:1315	the production	1302:1315	the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage	1302:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	3	19	theme	hot	215:217	arg1	extraction					225:234	the hot water extraction	211:234	the hot water extraction	211:234	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	11	20	dep	suggested	1348:1356	arg1	induced					1377:1383	induced	1377:1383	suggested the polysaccharide induced the functional activation of macrophage	1348:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	7	21	theme	in	778:779	arg1	experiment					787:796	The in vitro experiment	774:796	The in vitro experiment	774:796	The in vitro experiment indicated that ARP enhanced B- and T-lymphocyte proliferation.
30553856	3	22	theme	water	219:223	arg1	extraction					225:234	the hot water extraction	211:234	the hot water extraction	211:234	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	6	23	theme	1,5‑	752:755	arg1	1,3‑linkages					760:771	1,3‑linkages	760:771	1,3‑linkages	760:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	23	theme	1,5‑	752:755	arg1	1,5‑					752:755	1,5‑	752:755	1,5‑	752:755	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	23	theme	1,5‑	752:755	arg1	1,6‑linkages					719:730	1,6‑linkages	719:730	1,6‑linkages	719:730	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	23	theme	1,5‑	752:755	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	11	24	theme	macrophage	1240:1249	arg1	activation					1226:1235	the activation	1222:1235	the activation of macrophage in vitro	1222:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	8	25	theme	cell	953:956	arg1	viability					958:966	the highest cell viability	941:966	the highest cell viability	941:966	A dose-dependent relationship was observed, and a dose of 200 μg/mL resulted in the highest cell viability.
30553856	0	26	theme	immunostimulatory	22:38	arg1	activities					40:49	immunostimulatory activities	22:49	immunostimulatory activities	22:49	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	11	27	theme	macrophage	1414:1423	arg1	activation					1400:1409	the functional activation	1385:1409	the functional activation of macrophage	1385:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	9	28	theme	B-	1078:1079	arg1	proliferation					1098:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	9	29	theme	enhanced	1069:1076	arg1	proliferation					1098:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	2	30	from	euchroma	146:153	arg1	polysaccharide					118:131	A polysaccharide	116:131	A polysaccharide from Arnebia euchroma (Royle)	116:161	A polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	3	31	theme	ethanol	237:243	arg1	precipitation					245:257	ethanol precipitation	237:257	ethanol precipitation	237:257	named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA.
30553856	9	32	theme	cytokine	1033:1040	arg1	proliferation					1098:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	9	32	theme	cytokine	1033:1040	arg1	IFN-γ					1057:1061	IFN-γ	1057:1061	IFN-γ	1057:1061	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	9	32	theme	cytokine	1033:1040	arg1	interferon-γ					1043:1054	interferon-γ	1043:1054	interferon-γ (IFN-γ)	1043:1062	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	9	32	theme	cytokine	1033:1040	arg1	production					1015:1024	the production	1011:1024	the production of the cytokine	1011:1040	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	8	33	theme	200 μg/mL	919:927	arg1	dose					911:914	a dose	909:914	a dose of 200 μg/mL	909:927	A dose-dependent relationship was observed, and a dose of 200 μg/mL resulted in the highest cell viability.
30553856	11	34	theme	TNF-α	1320:1324	arg1	production					1306:1315	the production	1302:1315	the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage	1302:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	4	35	theme	fraction	331:338	arg1	weight					302:307	The molecular weight	288:307	The molecular weight of the polysaccharide fraction of ARP	288:345	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	4	35	theme	fraction	331:338	arg1	1.23 × 104 Da					368:380	1.23 × 104 Da	368:380	1.23 × 104 Da	368:380	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	8	36	theme	highest	945:951	arg1	viability					958:966	the highest cell viability	941:966	the highest cell viability	941:966	A dose-dependent relationship was observed, and a dose of 200 μg/mL resulted in the highest cell viability.
30553856	4	37	theme	polysaccharide	316:329	arg1	fraction					331:338	the polysaccharide fraction	312:338	the polysaccharide fraction of ARP	312:345	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	0	38	theme	polysaccharide	56:69	arg1	Characterizations					0:16	Characterizations	0:16	Characterizations	0:16	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	0	38	theme	polysaccharide	56:69	arg1	activities					40:49	immunostimulatory activities	22:49	immunostimulatory activities	22:49	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	11	39	from	activation	1226:1235	arg1	experiments					1186:1196	The experiments	1182:1196	The experiments of the effect of ARP on the activation of macrophage in vitro	1182:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	11	40	theme	ARP	1215:1217	arg1	effect					1205:1210	the effect	1201:1210	the effect of ARP on the activation of macrophage in vitro	1201:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	4	41	theme	ARP	343:345	arg1	fraction					331:338	the polysaccharide fraction	312:338	the polysaccharide fraction of ARP	312:345	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	6	42	theme	1,6‑linkages	719:730	arg1	residues					707:714	Galp residues	702:714	Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages	702:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	42	theme	1,6‑linkages	719:730	arg1	1,6‑linkages					719:730	1,6‑linkages	719:730	1,6‑linkages	719:730	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	42	theme	1,6‑linkages	719:730	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	9	43	theme	T-lymphocyte	1085:1096	arg1	proliferation					1098:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	enhanced B- and T-lymphocyte proliferation	1069:1110	In addition, ARP significantly stimulated the production of the cytokine, interferon-γ (IFN-γ), and enhanced B- and T-lymphocyte proliferation.
30553856	4	44	from	curve	401:405	arg1	weight					302:307	The molecular weight	288:307	The molecular weight of the polysaccharide fraction of ARP	288:345	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	4	44	from	curve	401:405	arg1	1.23 × 104 Da					368:380	1.23 × 104 Da	368:380	1.23 × 104 Da	368:380	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	4	45	theme	dextran	421:427	arg1	standards					429:437	dextran standards	421:437	dextran standards	421:437	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	6	46	theme	residues	740:747	arg1	residues					707:714	Galp residues	702:714	Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages	702:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	46	theme	residues	740:747	arg1	1,6‑linkages					719:730	1,6‑linkages	719:730	1,6‑linkages	719:730	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	46	theme	residues	740:747	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	5	47	theme	Monosaccharide	440:453	arg1	analysis					467:474	Monosaccharide composition analysis	440:474	Monosaccharide composition analysis	440:474	Monosaccharide composition analysis revealed that ARP was composed of Gal, Ara, Glu, Man, Rha and Fuc at a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2.
30553856	11	48	from	effect	1205:1210	arg1	activation					1226:1235	the activation	1222:1235	the activation of macrophage in vitro	1222:1258	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	4	49	theme	molecular	292:300	arg1	weight					302:307	The molecular weight	288:307	The molecular weight of the polysaccharide fraction of ARP	288:345	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	4	49	theme	molecular	292:300	arg1	1.23 × 104 Da					368:380	1.23 × 104 Da	368:380	1.23 × 104 Da	368:380	The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards.
30553856	5	50	theme	composition	455:465	arg1	analysis					467:474	Monosaccharide composition analysis	440:474	Monosaccharide composition analysis	440:474	Monosaccharide composition analysis revealed that ARP was composed of Gal, Ara, Glu, Man, Rha and Fuc at a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2.
30553856	11	51	theme	IL-1β	1336:1340	arg1	production					1306:1315	the production	1302:1315	the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage	1302:1423	The experiments of the effect of ARP on the activation of macrophage in vitro indicated that ARP significantly enhanced the production of TNF-α, IL-6 and IL-1β which suggested the polysaccharide induced the functional activation of macrophage.
30553856	0	52	from	activities	40:49	arg1	Johnst					101:106	Johnst	101:106	Johnst	101:106	Characterizations and immunostimulatory activities of a polysaccharide from Arnebia euchroma (Royle) Johnst.
30553856	6	53	theme	Galp	702:705	arg1	residues					707:714	Galp residues	702:714	Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages	702:771	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	53	theme	Galp	702:705	arg1	1,6‑linkages					719:730	1,6‑linkages	719:730	1,6‑linkages	719:730	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	6	53	theme	Galp	702:705	arg1	residues					740:747	Ara residues	736:747	Ara residues	736:747	Methylation analysis suggested that ARP was likely an arabinogalactan and that its backbone mainly consisted of Galp residues of 1,6‑linkages and Ara residues of 1,5‑ or 1,3‑linkages.
30553856	10	54	theme	interleukin-2	1149:1161	arg1	production					1170:1179	interleukin-2 (IL-2) production	1149:1179	interleukin-2 (IL-2) production	1149:1179	Meanwhile, ARP had little effect on interleukin-2 (IL-2) production.
30553856	10	55	theme	little	1132:1137	arg1	effect					1139:1144	little effect	1132:1144	little effect	1132:1144	Meanwhile, ARP had little effect on interleukin-2 (IL-2) production.
29802685	5	0	theme	osteogenetic	803:814	arg1	expression					823:832	osteogenetic factor expression	803:832	osteogenetic factor expression	803:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	6	1	theme	microcomputed	957:969	arg1	tomography					971:980	a microcomputed tomography	955:980	a microcomputed tomography	955:980	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	7	2	theme	composite	1163:1171	arg1	coating					1173:1179	the GO/CS/HA composite coating	1150:1179	the GO/CS/HA composite coating	1150:1179	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	3	3	from	deposition	475:484	arg1	substrates					492:501	Ti substrates	489:501	Ti substrates	489:501	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	5	4	theme	mineralization	783:796	arg1	evaluations					710:720	evaluations	710:720	evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression	710:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	4	5	theme	coating	613:619	arg1	strength					586:593	bonding strength	578:593	bonding strength	578:593	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	4	5	theme	coating	613:619	arg1	wettability					561:571	wettability	561:571	wettability	561:571	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	4	5	theme	coating	613:619	arg1	morphology					530:539	surface morphology	522:539	surface morphology	522:539	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	4	5	theme	coating	613:619	arg1	composition					548:558	phase composition	542:558	phase composition	542:558	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	5	6	theme	factor	816:821	arg1	expression					823:832	osteogenetic factor expression	803:832	osteogenetic factor expression	803:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	7	7	theme	coating	1173:1179	arg1	strength					1138:1145	the wettability and bonding strength	1110:1145	the wettability and bonding strength of the GO/CS/HA composite coating	1110:1179	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	6	8	theme	osteogenetic	856:867	arg1	properties					869:878	the in vivo osteogenetic properties	844:878	the in vivo osteogenetic properties	844:878	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	10	9	theme	implants	1494:1501	arg1	field					1478:1482	the field	1474:1482	the field of dental implants	1474:1501	Consequently, GO/CS/HA-Ti may have potential applications in the field of dental implants.
29802685	4	10	theme	composite	603:611	arg1	coating					613:619	this composite coating	598:619	this composite coating	598:619	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	5	11	theme	differentiation	761:775	arg1	evaluations					710:720	evaluations	710:720	evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression	710:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	4	12	theme	phase	542:546	arg1	composition					548:558	phase composition	542:558	phase composition	542:558	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	2	13	mod	modification	285:296	arg3	surface					277:283	surface modification	277:296	surface modification of titanium	277:308	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	2	13	mod	modification	285:296	arg1	titanium					301:308	titanium	301:308	titanium	301:308	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	2	14	theme	titanium	301:308	arg1	Meanwhile					266:274	Meanwhile	266:274	Meanwhile	266:274	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	2	14	theme	titanium	301:308	arg1	modification					285:296	surface modification	277:296	surface modification of titanium	277:308	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	7	15	theme	CS/HA	1224:1228	arg1	coatings					1230:1237	CS/HA coatings	1224:1237	CS/HA coatings	1224:1237	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	9	16	theme	GO/CS/HA-Ti	1353:1363	arg1	implant					1365:1371	this GO/CS/HA-Ti implant	1348:1371	this GO/CS/HA-Ti implant	1348:1371	Additionally, this GO/CS/HA-Ti implant could enhance osseointegration in vivo.
29802685	7	17	theme	GO/CS/HA	1154:1161	arg1	coating					1173:1179	the GO/CS/HA composite coating	1150:1179	the GO/CS/HA composite coating	1150:1179	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	6	18	theme	in	848:849	arg1	properties					869:878	the in vivo osteogenetic properties	844:878	the in vivo osteogenetic properties	844:878	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	14	19	theme	Part	1633:1636	arg1	B					1638:1638	Part B	1633:1638	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.	1614:1674	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.
29802685	5	20	theme	cell	778:781	arg1	mineralization					783:796	cell mineralization	778:796	cell mineralization	778:796	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	6	21	theme	histological	932:943	arg1	analysis					945:952	a histological analysis	930:952	a histological analysis	930:952	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	5	22	theme	in	652:653	arg1	examination					673:683	in vitro cytological examination	652:683	in vitro cytological examination	652:683	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	3	23	theme	electrophoretic	459:473	arg1	deposition					475:484	electrophoretic deposition	459:484	electrophoretic deposition on Ti substrates	459:501	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	0	24	theme	electrophoretic	127:141	arg1	deposition					143:152	electrophoretic deposition	127:152	electrophoretic deposition	127:152	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	4	25	theme	surface	522:528	arg1	morphology					530:539	surface morphology	522:539	surface morphology	522:539	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	5	26	theme	cytological	661:671	arg1	examination					673:683	in vitro cytological examination	652:683	in vitro cytological examination	652:683	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	7	27	theme	wettability	1114:1124	arg1	strength					1138:1145	the wettability and bonding strength	1110:1145	the wettability and bonding strength of the GO/CS/HA composite coating	1110:1179	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	2	28	theme	surface	277:283	arg1	Meanwhile					266:274	Meanwhile	266:274	Meanwhile	266:274	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	2	28	theme	surface	277:283	arg1	modification					285:296	surface modification	277:296	surface modification of titanium	277:308	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	14	29	dep	Res	1629:1631	arg1	Biomater					1646:1653	Biomater	1646:1653	Biomater	1646:1653	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.
29802685	14	29	dep	Res	1629:1631	arg1	2019					1670:1673	2019	1670:1673	2019	1670:1673	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.
29802685	14	29	dep	Res	1629:1631	arg1	B					1638:1638	Part B	1633:1638	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.	1614:1674	J Biomed Mater Res Part B: Appl Biomater 107B: 635-645, 2019.
29802685	13	30	dep	©	1583:1583	arg1	Inc.					1609:1612	Inc.	1609:1612	Inc.	1609:1612	© 2018 Wiley Periodicals, Inc.
29802685	3	31	theme	GO/CS/HA	413:420	arg1	coating					433:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	7	32	theme	bonding	1130:1136	arg1	strength					1138:1145	the wettability and bonding strength	1110:1145	the wettability and bonding strength of the GO/CS/HA composite coating	1110:1179	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	0	33	theme	graphene	44:51	arg1	coating					93:99	a graphene oxide/chitosan/hydroxyapatite composite coating	42:99	a graphene oxide/chitosan/hydroxyapatite composite coating on titanium	42:111	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	1	34	theme	implant	198:204	arg1	Titanium					155:162	Titanium	155:162	Titanium (Ti)	155:167	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	1	34	theme	implant	198:204	arg1	material					206:213	an implant material	195:213	an implant material in dentistry and bone surgery	195:243	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	0	35	theme	osseointegration	19:34	arg1	enhancement					4:14	The enhancement	0:14	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium	0:111	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	4	36	theme	bonding	578:584	arg1	strength					586:593	bonding strength	578:593	bonding strength	578:593	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	5	37	theme	cell	740:743	arg1	viability					745:753	cell viability	740:753	cell viability	740:753	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	3	38	theme	oxide/chitosan/hydroxyapatite	382:410	arg1	coating					433:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	5	39	theme	viability	745:753	arg1	evaluations					710:720	evaluations	710:720	evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression	710:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	6	40	dep	in	848:849	arg1	vivo					851:854	vivo	851:854	vivo	851:854	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	5	41	theme	expression	823:832	arg1	evaluations					710:720	evaluations	710:720	evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression	710:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	12	42	theme	Part	1554:1557	arg1	B					1559:1559	Part B	1554:1559	J Biomed Mater Res Part B: Appl Biomater, 2018.	1535:1581	J Biomed Mater Res Part B: Appl Biomater, 2018.
29802685	8	43	theme	cell-material	1297:1309	arg1	interactions					1311:1322	the cell-material interactions	1293:1322	the cell-material interactions	1293:1322	Furthermore, the GO/CS/HA coating greatly heightened the cell-material interactions in vitro.
29802685	5	44	theme	cell	756:759	arg1	differentiation					761:775	cell differentiation	756:775	cell differentiation	756:775	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	0	45	from	coating	93:99	arg1	titanium					104:111	titanium	104:111	titanium	104:111	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	3	46	theme	graphene	373:380	arg1	coating					433:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	7	47	theme	composite	1070:1078	arg1	coating					1080:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	3	48	theme	Ti	489:490	arg1	substrates					492:501	Ti substrates	489:501	Ti substrates	489:501	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	0	49	theme	oxide/chitosan/hydroxyapatite	53:81	arg1	coating					93:99	a graphene oxide/chitosan/hydroxyapatite composite coating	42:99	a graphene oxide/chitosan/hydroxyapatite composite coating on titanium	42:111	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	6	50	theme	biomechanical	987:999	arg1	tests					1001:1005	biomechanical tests	987:1005	biomechanical tests	987:1005	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	7	51	theme	crack-free	1050:1059	arg1	coating					1080:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	3	52	theme	composite	423:431	arg1	coating					433:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating	371:439	In this study, a graphene oxide/chitosan/hydroxyapatite (GO/CS/HA) composite coating was fabricated by electrophoretic deposition on Ti substrates.
29802685	5	53	theme	cell	725:728	arg1	adhesion					730:737	cell adhesion	725:737	cell adhesion	725:737	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	1	54	from	material	206:213	arg1	bone					232:235	bone	232:235	bone	232:235	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	1	54	from	material	206:213	arg1	dentistry					218:226	dentistry	218:226	dentistry	218:226	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	6	55	theme	animal	906:911	arg1	study					913:917	an animal study	903:917	an animal study	903:917	Finally, the in vivo osteogenetic properties were evaluated through an animal study, including a histological analysis, a microcomputed tomography, and biomechanical tests.
29802685	4	56	dep	morphology	530:539	arg1	the					518:520	the	518:520	the	518:520	Subsequently, the surface morphology, phase composition, wettability, and bonding strength of this composite coating were researched.
29802685	5	57	theme	adhesion	730:737	arg1	evaluations					710:720	evaluations	710:720	evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression	710:832	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	10	58	theme	dental	1487:1492	arg1	implants					1494:1501	dental implants	1487:1501	dental implants	1487:1501	Consequently, GO/CS/HA-Ti may have potential applications in the field of dental implants.
29802685	1	59	dep	dentistry	218:226	arg1	surgery					237:243	surgery	237:243	surgery	237:243	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	7	60	theme	GO/CS/HA	1061:1068	arg1	coating					1080:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	2	61	theme	implants	346:353	arg1	osseointegration					326:341	the osseointegration	322:341	the osseointegration of implants	322:353	Meanwhile, surface modification of titanium can enhance the osseointegration of implants.
29802685	5	62	dep	in	652:653	arg1	vitro					655:659	vitro	655:659	vitro	655:659	Additionally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell viability, cell differentiation, cell mineralization, and osteogenetic factor expression.
29802685	10	63	contain	have	1443:1446	arg1	GO/CS/HA-Ti					1427:1437	GO/CS/HA-Ti	1427:1437	GO/CS/HA-Ti	1427:1437	Consequently, GO/CS/HA-Ti may have potential applications in the field of dental implants.
29802685	10	63	contain	have	1443:1446	arg2	applications					1458:1469	potential applications	1448:1469	potential applications	1448:1469	Consequently, GO/CS/HA-Ti may have potential applications in the field of dental implants.
29802685	7	64	theme	homogeneous	1034:1044	arg1	coating					1080:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	a homogeneous and crack-free GO/CS/HA composite coating	1032:1086	The results showed that a homogeneous and crack-free GO/CS/HA composite coating was coated on Ti, and the wettability and bonding strength of the GO/CS/HA composite coating were enhanced compared with HA, GO/HA, and CS/HA coatings.
29802685	8	65	theme	GO/CS/HA	1257:1264	arg1	coating					1266:1272	the GO/CS/HA coating	1253:1272	the GO/CS/HA coating	1253:1272	Furthermore, the GO/CS/HA coating greatly heightened the cell-material interactions in vitro.
29802685	11	66	dep	©	1504:1504	arg1	Inc.					1530:1533	Inc.	1530:1533	Inc.	1530:1533	© 2018 Wiley Periodicals, Inc.
29802685	10	67	theme	potential	1448:1456	arg1	applications					1458:1469	potential applications	1448:1469	potential applications	1448:1469	Consequently, GO/CS/HA-Ti may have potential applications in the field of dental implants.
29802685	0	68	theme	composite	83:91	arg1	coating					93:99	a graphene oxide/chitosan/hydroxyapatite composite coating	42:99	a graphene oxide/chitosan/hydroxyapatite composite coating on titanium	42:111	The enhancement of osseointegration using a graphene oxide/chitosan/hydroxyapatite composite coating on titanium fabricated by electrophoretic deposition.
29802685	1	69	used	used	187:190	arg2	Ti					165:166	Ti	165:166	Ti	165:166	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	1	69	used	used	187:190	arg2	material					206:213	an implant material	195:213	an implant material in dentistry and bone surgery	195:243	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	1	69	used	used	187:190	arg2	Titanium					155:162	Titanium	155:162	Titanium (Ti)	155:167	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29802685	12	70	dep	Res	1550:1552	arg1	2018					1577:1580	2018	1577:1580	2018	1577:1580	J Biomed Mater Res Part B: Appl Biomater, 2018.
29802685	12	70	dep	Res	1550:1552	arg1	B					1559:1559	Part B	1554:1559	J Biomed Mater Res Part B: Appl Biomater, 2018.	1535:1581	J Biomed Mater Res Part B: Appl Biomater, 2018.
29802685	12	70	dep	Res	1550:1552	arg1	Biomater					1567:1574	Biomater	1567:1574	Biomater	1567:1574	J Biomed Mater Res Part B: Appl Biomater, 2018.
29802685	1	71	theme	several	249:255	arg1	decades					257:263	several decades	249:263	several decades	249:263	Titanium (Ti) has been commonly used as an implant material in dentistry and bone surgery for several decades.
29486259	0	0	theme	plumbum	100:106	arg1	detection					113:121	detection	113:121	detection	113:121	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	0	theme	plumbum	100:106	arg1	validation					131:140	its validation	127:140	its validation in blood serum	127:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	0	theme	plumbum	100:106	arg1	ions					108:111	trace plumbum ions	94:111	trace plumbum ions detection and its validation in blood serum	94:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	1	1	theme	ions	343:346	arg1	analysis					318:325	the analysis	314:325	the analysis of trace Pb(II) ions detection and its validation in blood serum	314:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	6	2	theme	trace	1071:1075	arg1	ions					1084:1087	trace Pb(II) ions	1071:1087	trace Pb(II) ions detection in complex medium such as blood serum	1071:1135	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	1	3	theme	novel	160:164	arg1	synthesis					166:174	novel synthesis	160:174	novel synthesis	160:174	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	0	4	theme	trace	94:98	arg1	detection					113:121	detection	113:121	detection	113:121	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	4	theme	trace	94:98	arg1	validation					131:140	its validation	127:140	its validation in blood serum	127:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	4	theme	trace	94:98	arg1	ions					108:111	trace plumbum ions	94:111	trace plumbum ions detection and its validation in blood serum	94:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	6	5	theme	Quantification	1258:1271	arg1	Detection					1213:1221	Detection	1213:1221	Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb	1213:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	5	theme	Quantification	1258:1271	arg1	LOD					1224:1226	LOD	1224:1226	LOD	1224:1226	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	6	theme	complex	1102:1108	arg1	medium					1110:1115	complex medium	1102:1115	complex medium	1102:1115	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	3	7	theme	functional	655:664	arg1	groups					666:671	functional groups	655:671	functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding	655:759	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	1	8	theme	composite	287:295	arg1	characterization					180:195	characterization	180:195	characterization	180:195	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	8	theme	composite	287:295	arg1	synthesis					166:174	novel synthesis	160:174	novel synthesis	160:174	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	2	9	theme	scattered	531:539	arg1	agglomeration					555:567	scattered particle size agglomeration	531:567	scattered particle size agglomeration	531:567	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	7	10	theme	clinical	1467:1474	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	6	11	theme	Detection	1213:1221	arg1	Limit					1204:1208	resulting Limit	1194:1208	resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb	1194:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	12	theme	10-60ppb	1179:1186	arg1	range					1170:1174	the physiologically relevant range	1141:1174	the physiologically relevant range of 10-60ppb	1141:1186	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	13	from	detection	1089:1097	arg1	medium					1110:1115	complex medium	1102:1115	complex medium	1102:1115	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	3	14	dep	bending	693:699	arg1	the					689:691	the	689:691	the	689:691	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	5	15	theme	thermal	965:971	arg1	stability					973:981	thermal stability	965:981	thermal stability	965:981	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	7	16	theme	rapid	1443:1447	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	6	17	theme	Limit	1249:1253	arg1	Detection					1213:1221	Detection	1213:1221	Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb	1213:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	17	theme	Limit	1249:1253	arg1	LOD					1224:1226	LOD	1224:1226	LOD	1224:1226	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	7	18	theme	robust	1423:1428	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	5	19	from	700°C	1022:1026	arg1	content					1011:1017	more than 60% residual content	988:1017	more than 60% residual content at 700°C	988:1026	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	7	20	theme	electrochemical	1387:1401	arg1	characteristics					1403:1417	favourable electrochemical characteristics	1376:1417	favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1376:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	7	21	theme	environmental	1449:1461	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	2	22	theme	Electron	421:428	arg1	FESEM					442:446	FESEM	442:446	FESEM	442:446	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	2	22	theme	Electron	421:428	arg1	Microscopy					430:439	Field Emission Scanning Electron Microscopy	397:439	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	7	23	dep	in	1431:1432	arg1	situ					1434:1437	situ	1434:1437	situ	1434:1437	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	2	24	theme	Field	397:401	arg1	FESEM					442:446	FESEM	442:446	FESEM	442:446	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	2	24	theme	Field	397:401	arg1	Microscopy					430:439	Field Emission Scanning Electron Microscopy	397:439	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	7	25	with	platform	1362:1369	arg1	characteristics					1403:1417	favourable electrochemical characteristics	1376:1417	favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1376:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	6	26	theme	0.11±0.36ppb	1232:1243	arg1	Detection					1213:1221	Detection	1213:1221	Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb	1213:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	26	theme	0.11±0.36ppb	1232:1243	arg1	LOD					1224:1226	LOD	1224:1226	LOD	1224:1226	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	0	27	dep	ions	108:111	arg1	detection					113:121	detection	113:121	detection	113:121	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	27	dep	ions	108:111	arg1	validation					131:140	its validation	127:140	its validation in blood serum	127:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	27	dep	ions	108:111	arg1	ions					108:111	trace plumbum ions	94:111	trace plumbum ions detection and its validation in blood serum	94:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	7	28	theme	fabricated	1306:1315	arg1	electrode					1317:1325	The newly fabricated electrode	1296:1325	The newly fabricated electrode	1296:1325	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	7	28	theme	fabricated	1306:1315	arg1	advantageous					1336:1347	advantageous	1336:1347	advantageous	1336:1347	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	1	29	theme	blood	380:384	arg1	serum					386:390	blood serum	380:390	blood serum	380:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	4	30	theme	electrode	884:892	arg1	properties					866:875	the properties	862:875	the properties of the electrode	862:892	X-ray Diffraction (XRD) analyses further elucidated that the crystallite size could have influenced the properties of the electrode.
29486259	4	31	theme	Diffraction	768:778	arg1	analyses					786:793	X-ray Diffraction (XRD) analyses	762:793	X-ray Diffraction (XRD) analyses	762:793	X-ray Diffraction (XRD) analyses further elucidated that the crystallite size could have influenced the properties of the electrode.
29486259	6	32	theme	II	1080:1081	arg1	ions					1084:1087	trace Pb(II) ions	1071:1087	trace Pb(II) ions detection in complex medium such as blood serum	1071:1135	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	7	33	theme	metal	1494:1498	arg1	ions					1500:1503	heavy metal ions	1488:1503	heavy metal ions	1488:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	1	34	dep	synthesis	166:174	arg1	A					158:158	A	158:158	A	158:158	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	7	35	theme	in	1431:1432	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	1	36	theme	cellulose	200:208	arg1	characterization					180:195	characterization	180:195	characterization	180:195	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	36	theme	cellulose	200:208	arg1	synthesis					166:174	novel synthesis	160:174	novel synthesis	160:174	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	4	37	theme	X-ray	762:766	arg1	XRD					781:783	XRD	781:783	XRD	781:783	X-ray Diffraction (XRD) analyses further elucidated that the crystallite size could have influenced the properties of the electrode.
29486259	4	37	theme	X-ray	762:766	arg1	Diffraction					768:778	X-ray Diffraction	762:778	X-ray Diffraction (XRD) analyses	762:793	X-ray Diffraction (XRD) analyses further elucidated that the crystallite size could have influenced the properties of the electrode.
29486259	7	38	theme	sensing	1354:1360	arg1	platform					1362:1369	a sensing platform	1352:1369	a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1352:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	2	39	theme	Microscopy	430:439	arg1	analyses					449:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	1	40	from	validation	366:375	arg1	serum					386:390	blood serum	380:390	blood serum	380:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	3	41	theme	groups	666:671	arg1	presence					643:650	the presence	639:650	the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding	639:759	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	7	42	theme	favourable	1376:1385	arg1	characteristics					1403:1417	favourable electrochemical characteristics	1376:1417	favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1376:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	1	43	theme	hydroxyapatite	211:224	arg1	characterization					180:195	characterization	180:195	characterization	180:195	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	43	theme	hydroxyapatite	211:224	arg1	synthesis					166:174	novel synthesis	160:174	novel synthesis	160:174	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	44	from	detection	348:356	arg1	serum					386:390	blood serum	380:390	blood serum	380:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	45	dep	ions	343:346	arg1	detection					348:356	detection	348:356	detection	348:356	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	45	dep	ions	343:346	arg1	validation					366:375	its validation	362:375	its validation in blood serum	362:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	45	dep	ions	343:346	arg1	ions					343:346	trace Pb(II) ions detection and its validation in blood serum	330:390	trace Pb(II) ions detection and its validation in blood serum	330:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	2	46	theme	size	550:553	arg1	agglomeration					555:567	scattered particle size agglomeration	531:567	scattered particle size agglomeration	531:567	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	5	47	dep	content	1011:1017	arg1	%					1000:1000	%	1000:1000	%	1000:1000	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	4	48	theme	crystallite	823:833	arg1	size					835:838	the crystallite size	819:838	the crystallite size	819:838	X-ray Diffraction (XRD) analyses further elucidated that the crystallite size could have influenced the properties of the electrode.
29486259	5	49	theme	Thermo-gravimetric	904:921	arg1	TGA/DTG					933:939	TGA/DTG	933:939	TGA/DTG	933:939	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	5	49	theme	Thermo-gravimetric	904:921	arg1	Analysis					923:930	Thermo-gravimetric Analysis	904:930	Thermo-gravimetric Analysis (TGA/DTG)	904:940	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	2	50	theme	Scanning	412:419	arg1	FESEM					442:446	FESEM	442:446	FESEM	442:446	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	2	50	theme	Scanning	412:419	arg1	Microscopy					430:439	Field Emission Scanning Electron Microscopy	397:439	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	0	51	theme	cellulose	34:42	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	51	theme	cellulose	34:42	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	1	52	theme	trace	330:334	arg1	detection					348:356	detection	348:356	detection	348:356	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	52	theme	trace	330:334	arg1	validation					366:375	its validation	362:375	its validation in blood serum	362:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	52	theme	trace	330:334	arg1	ions					343:346	trace Pb(II) ions detection and its validation in blood serum	330:390	trace Pb(II) ions detection and its validation in blood serum	330:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	0	53	theme	blood	145:149	arg1	serum					151:155	blood serum	145:155	blood serum	145:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	6	54	theme	ions	1084:1087	arg1	detection					1089:1097	trace Pb(II) ions detection	1071:1097	trace Pb(II) ions detection in complex medium such as blood serum	1071:1135	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	2	55	theme	particle	541:548	arg1	agglomeration					555:567	scattered particle size agglomeration	531:567	scattered particle size agglomeration	531:567	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	1	56	theme	chemically-modified	230:248	arg1	composite					287:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	0	57	theme	electrode	70:78	arg1	composite					80:88	hydroxyapatite-carbon electrode composite	48:88	hydroxyapatite-carbon electrode composite	48:88	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	1	58	theme	Pb	336:337	arg1	detection					348:356	detection	348:356	detection	348:356	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	58	theme	Pb	336:337	arg1	validation					366:375	its validation	362:375	its validation in blood serum	362:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	58	theme	Pb	336:337	arg1	ions					343:346	trace Pb(II) ions detection and its validation in blood serum	330:390	trace Pb(II) ions detection and its validation in blood serum	330:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	6	59	theme	0.36±0.36ppb	1282:1293	arg1	LOQ					1274:1276	LOQ	1274:1276	LOQ	1274:1276	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	6	59	theme	0.36±0.36ppb	1282:1293	arg1	Quantification					1258:1271	Quantification	1258:1271	Quantification (LOQ) of 0.36±0.36ppb	1258:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	2	60	theme	orderly	497:503	arg1	structure					512:520	the orderly porous structure	493:520	the orderly porous structure	493:520	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	3	61	theme	Spectroscopy	601:612	arg1	spectra					621:627	Infrared Spectroscopy (FTIR) spectra	592:627	Infrared Spectroscopy (FTIR) spectra	592:627	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	6	62	theme	relevant	1161:1168	arg1	range					1170:1174	the physiologically relevant range	1141:1174	the physiologically relevant range of 10-60ppb	1141:1186	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	5	63	dep	%	1000:1000	arg1	60					998:999	60	998:999	60	998:999	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	0	64	theme	hydroxyapatite-carbon	48:68	arg1	composite					80:88	hydroxyapatite-carbon electrode composite	48:88	hydroxyapatite-carbon electrode composite	48:88	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	7	65	theme	ions	1500:1503	arg1	analyses					1476:1483	robust, in situ and rapid environmental and clinical analyses	1423:1483	robust, in situ and rapid environmental and clinical analyses of heavy metal ions	1423:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	6	66	theme	blood	1125:1129	arg1	serum					1131:1135	blood serum	1125:1135	blood serum	1125:1135	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	1	67	theme	II	339:340	arg1	detection					348:356	detection	348:356	detection	348:356	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	67	theme	II	339:340	arg1	validation					366:375	its validation	362:375	its validation in blood serum	362:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	1	67	theme	II	339:340	arg1	ions					343:346	trace Pb(II) ions detection and its validation in blood serum	330:390	trace Pb(II) ions detection and its validation in blood serum	330:390	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	7	68	theme	heavy	1488:1492	arg1	ions					1500:1503	heavy metal ions	1488:1503	heavy metal ions	1488:1503	The newly fabricated electrode could be advantageous as a sensing platform with favourable electrochemical characteristics for robust, in situ and rapid environmental and clinical analyses of heavy metal ions.
29486259	1	69	theme	carbon	250:255	arg1	composite					287:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	5	70	theme	residual	1002:1009	arg1	content					1011:1017	more than 60% residual content	988:1017	more than 60% residual content at 700°C	988:1026	Based on Thermo-gravimetric Analysis (TGA/DTG), the composites showed thermal stability with more than 60% residual content at 700°C.
29486259	0	71	from	detection	113:121	arg1	serum					151:155	blood serum	145:155	blood serum	145:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	6	72	theme	resulting	1194:1202	arg1	Limit					1204:1208	resulting Limit	1194:1208	resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb	1194:1293	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	3	73	dep	Fourier	574:580	arg1	Transform					582:590	Transform	582:590	Transform Infrared Spectroscopy (FTIR) spectra	582:627	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	1	74	theme	electrode	257:265	arg1	composite					287:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
29486259	2	75	theme	porous	505:510	arg1	structure					512:520	the orderly porous structure	493:520	the orderly porous structure	493:520	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	2	76	theme	Emission	403:410	arg1	FESEM					442:446	FESEM	442:446	FESEM	442:446	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	2	76	theme	Emission	403:410	arg1	Microscopy					430:439	Field Emission Scanning Electron Microscopy	397:439	The Field Emission Scanning Electron Microscopy (FESEM) analyses	393:456	The Field Emission Scanning Electron Microscopy (FESEM) analyses showed that the composite retained the orderly porous structure but with scattered particle size agglomeration.
29486259	6	77	theme	Pb	1077:1078	arg1	ions					1084:1087	trace Pb(II) ions	1071:1087	trace Pb(II) ions detection in complex medium such as blood serum	1071:1135	The sensor was successfully developed for trace Pb(II) ions detection in complex medium such as blood serum, in the physiologically relevant range of 10-60ppb, with resulting Limit of Detection (LOD) of 0.11±0.36ppb and Limit of Quantification (LOQ) of 0.36±0.36ppb.
29486259	0	78	theme	composite	80:88	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	0	78	theme	composite	80:88	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	3	79	theme	carbon	719:724	arg1	bonds					726:730	carbon bonds	719:730	carbon bonds	719:730	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	0	80	from	validation	131:140	arg1	serum					151:155	blood serum	145:155	blood serum	145:155	Synthesis and characterization of cellulose and hydroxyapatite-carbon electrode composite for trace plumbum ions detection and its validation in blood serum.
29486259	3	81	theme	bonds	726:730	arg1	stretching					705:714	stretching	705:714	stretching	705:714	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	3	81	theme	bonds	726:730	arg1	bending					693:699	bending	693:699	bending	693:699	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	3	82	theme	Infrared	592:599	arg1	FTIR					615:618	FTIR	615:618	FTIR	615:618	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	3	82	theme	Infrared	592:599	arg1	Spectroscopy					601:612	Infrared Spectroscopy	592:612	Infrared Spectroscopy (FTIR) spectra	592:627	The Fourier Transform Infrared Spectroscopy (FTIR) spectra suggested the presence of functional groups associated with the bending and stretching of carbon bonds and intermolecular H-bonding.
29486259	1	83	theme	Cellulose-HAp-CME	268:284	arg1	composite					287:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	chemically-modified carbon electrode (Cellulose-HAp-CME) composite	230:295	A novel synthesis and characterization of cellulose, hydroxyapatite and chemically-modified carbon electrode (Cellulose-HAp-CME) composite was reported for the analysis of trace Pb(II) ions detection and its validation in blood serum.
31892123	0	0	theme	Biopolymer-Based	86:101	arg1	Scaffolds					110:118	Biopolymer-Based Porous Scaffolds	86:118	Biopolymer-Based Porous Scaffolds	86:118	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	2	1	theme	novel	432:436	arg1	procedure					471:479	a novel wet-chemical and low-temperature procedure	430:479	a novel wet-chemical and low-temperature procedure	430:479	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	5	2	with	scaffolds	898:906	arg1	sizes					926:930	varying pore sizes	913:930	varying pore sizes	913:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	5	3	theme	In	843:844	arg1	studies					852:858	In vitro studies	843:858	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes	843:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	4	4	theme	aspect	782:787	arg1	>					795:795	an aspect ratio > 10	779:798	an aspect ratio > 10	779:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	5	5	theme	developed	983:991	arg1	procedure					993:1001	the developed procedure	979:1001	the developed procedure	979:1001	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	0	6	theme	Scaffolds	110:118	arg1	Fabrication					71:81	the Fabrication	67:81	the Fabrication of Biopolymer-Based Porous Scaffolds	67:118	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	5	7	from	studies	852:858	arg1	scaffolds					898:906	the bioactive biopolymer composite scaffolds	863:906	the bioactive biopolymer composite scaffolds with varying pore sizes	863:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	4	8	theme	SDS	838:840	arg1	presence					826:833	the presence	822:833	the presence of SDS	822:840	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	0	9	theme	Porous	103:108	arg1	Scaffolds					110:118	Biopolymer-Based Porous Scaffolds	86:118	Biopolymer-Based Porous Scaffolds	86:118	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	4	10	theme	ratio	789:793	arg1	>					795:795	an aspect ratio > 10	779:798	an aspect ratio > 10	779:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	4	11	theme	bioactive	737:745	arg1	nanorods					765:772	highly bioactive and monophasic HA nanorods	730:772	highly bioactive and monophasic HA nanorods with an aspect ratio > 10	730:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	5	12	theme	pore	921:924	arg1	sizes					926:930	varying pore sizes	913:930	varying pore sizes	913:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	0	13	from	Wastes	56:61	arg1	Extraction					0:9	Extraction	0:9	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.	0:119	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	5	14	theme	varying	913:919	arg1	sizes					926:930	varying pore sizes	913:930	varying pore sizes	913:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	2	15	theme	marine	486:491	arg1	wastes					493:498	marine wastes	486:498	marine wastes containing calcium carbonates	486:528	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	5	16	theme	profitable	1031:1040	arg1	composites					1042:1051	profitable composites	1031:1051	profitable composites for tissue engineering	1031:1074	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	5	17	theme	bioactive	867:875	arg1	scaffolds					898:906	the bioactive biopolymer composite scaffolds	863:906	the bioactive biopolymer composite scaffolds with varying pore sizes	863:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	3	18	theme	hexadecyltrimethylammonium	614:639	arg1	CTAB					650:653	CTAB	650:653	CTAB	650:653	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	18	theme	hexadecyltrimethylammonium	614:639	arg1	bromide					641:647	hexadecyltrimethylammonium bromide	614:647	hexadecyltrimethylammonium bromide (CTAB)	614:654	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	2	19	theme	ceramic	390:396	arg1	nanostructures					398:411	The bioactive ceramic nanostructures	376:411	The bioactive ceramic nanostructures	376:411	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	1	20	theme	carboxylic	228:237	arg1	acids					239:243	carboxylic acids	228:243	carboxylic acids biopolymers	228:255	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	5	21	theme	biopolymer	877:886	arg1	scaffolds					898:906	the bioactive biopolymer composite scaffolds	863:906	the bioactive biopolymer composite scaffolds with varying pore sizes	863:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	2	22	theme	bioactive	380:388	arg1	nanostructures					398:411	The bioactive ceramic nanostructures	376:411	The bioactive ceramic nanostructures	376:411	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	2	23	theme	low-temperature	455:469	arg1	procedure					471:479	a novel wet-chemical and low-temperature procedure	430:479	a novel wet-chemical and low-temperature procedure	430:479	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	0	24	theme	Nanostructures	29:42	arg1	Extraction					0:9	Extraction	0:9	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.	0:119	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	5	25	dep	300	945:947	arg1	to					942:943	to	942:943	to	942:943	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	0	26	theme	Hydroxyapatite	14:27	arg1	Nanostructures					29:42	Hydroxyapatite Nanostructures	14:42	Hydroxyapatite Nanostructures	14:42	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	1	27	theme	soft-bone-tissue	346:361	arg1	engineering					363:373	soft-bone-tissue engineering	346:373	soft-bone-tissue engineering	346:373	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	5	28	theme	procedure	993:1001	arg1	capacity					967:974	the capacity	963:974	the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering	963:1074	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	4	29	with	nanorods	765:772	arg1	>					795:795	an aspect ratio > 10	779:798	an aspect ratio > 10	779:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	1	30	theme	Three-dimensional	121:137	arg1	nanocomposites					146:159	Three-dimensional porous nanocomposites	121:159	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures	121:305	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	2	31	theme	calcium	511:517	arg1	carbonates					519:528	calcium carbonates	511:528	calcium carbonates	511:528	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	0	32	theme	Marine	49:54	arg1	Wastes					56:61	Marine Wastes	49:61	Marine Wastes	49:61	Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.
31892123	5	33	dep	In	843:844	arg1	vitro					846:850	vitro	846:850	vitro	846:850	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	3	34	theme	surface-active	545:558	arg1	sulfate					596:602	sodium dodecyl sulfate	581:602	sodium dodecyl sulfate (SDS)	581:608	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	34	theme	surface-active	545:558	arg1	molecules					560:568	surface-active molecules	545:568	surface-active molecules	545:568	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	34	theme	surface-active	545:558	arg1	bromide					641:647	hexadecyltrimethylammonium bromide	614:647	hexadecyltrimethylammonium bromide (CTAB)	614:654	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	35	theme	nanostructures	681:694	arg1	morphology					664:673	the morphology	660:673	the morphology of HA nanostructures	660:694	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	1	36	theme	acids	239:243	arg1	biopolymers					245:255	carboxylic acids biopolymers	228:255	carboxylic acids biopolymers	228:255	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	1	37	theme	monophasic	261:270	arg1	HA					288:289	HA	288:289	HA	288:289	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	1	37	theme	monophasic	261:270	arg1	hydroxyapatite					272:285	monophasic hydroxyapatite	261:285	monophasic hydroxyapatite (HA) nanostructures	261:305	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	4	38	theme	monophasic	751:760	arg1	nanorods					765:772	highly bioactive and monophasic HA nanorods	730:772	highly bioactive and monophasic HA nanorods with an aspect ratio > 10	730:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	5	39	theme	composite	888:896	arg1	scaffolds					898:906	the bioactive biopolymer composite scaffolds	863:906	the bioactive biopolymer composite scaffolds with varying pore sizes	863:930	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	1	40	theme	hydroxyapatite	272:285	arg1	nanostructures					292:305	monophasic hydroxyapatite (HA) nanostructures	261:305	monophasic hydroxyapatite (HA) nanostructures	261:305	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	1	41	theme	porous	139:144	arg1	nanocomposites					146:159	Three-dimensional porous nanocomposites	121:159	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures	121:305	Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering.
31892123	3	42	theme	HA	678:679	arg1	nanostructures					681:694	HA nanostructures	678:694	HA nanostructures	678:694	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	4	43	theme	HA	762:763	arg1	nanorods					765:772	highly bioactive and monophasic HA nanorods	730:772	highly bioactive and monophasic HA nanorods with an aspect ratio > 10	730:798	It is demonstrated that highly bioactive and monophasic HA nanorods with an aspect ratio > 10 can be synthesized in the presence of SDS.
31892123	2	44	contain	containing	500:509	arg2	carbonates					519:528	calcium carbonates	511:528	calcium carbonates	511:528	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	2	44	contain	containing	500:509	arg1	wastes					493:498	marine wastes	486:498	marine wastes containing calcium carbonates	486:528	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31892123	3	45	theme	sodium	581:586	arg1	sulfate					596:602	sodium dodecyl sulfate	581:602	sodium dodecyl sulfate (SDS)	581:608	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	45	theme	sodium	581:586	arg1	SDS					605:607	SDS	605:607	SDS	605:607	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	5	46	theme	marine	1014:1019	arg1	wastes					1021:1026	marine wastes	1014:1026	marine wastes	1014:1026	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	3	47	theme	dodecyl	588:594	arg1	sulfate					596:602	sodium dodecyl sulfate	581:602	sodium dodecyl sulfate (SDS)	581:608	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	47	theme	dodecyl	588:594	arg1	SDS					605:607	SDS	605:607	SDS	605:607	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	3	48	theme	molecules	560:568	arg1	effect					535:540	The effect	531:540	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures	531:694	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	5	49	theme	tissue	1057:1062	arg1	engineering					1064:1074	tissue engineering	1057:1074	tissue engineering	1057:1074	In vitro studies on the bioactive biopolymer composite scaffolds with varying pore sizes, from 100 to 300 μm, determine the capacity of the developed procedure to convert marine wastes to profitable composites for tissue engineering.
31892123	3	50	from	effect	535:540	arg1	morphology					664:673	the morphology	660:673	the morphology of HA nanostructures	660:694	The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.
31892123	2	51	theme	wet-chemical	438:449	arg1	procedure					471:479	a novel wet-chemical and low-temperature procedure	430:479	a novel wet-chemical and low-temperature procedure	430:479	The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates.
31356940	1	0	theme	oligosaccharides	169:184	arg1	characterization					131:146	characterization	131:146	characterization	131:146	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	1	0	theme	oligosaccharides	169:184	arg1	concentration					152:164	concentration	152:164	concentration	152:164	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	5	1	theme	oligosaccharides	795:810	arg1	source					785:790	a source	783:790	a source of oligosaccharides with potential functional and possible application for human nutrition	783:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	5	1	theme	oligosaccharides	795:810	arg1	whey					763:766	goat whey	758:766	goat whey	758:766	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	5	2	used	used	775:778	arg2	whey					763:766	goat whey	758:766	goat whey	758:766	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	5	2	used	used	775:778	arg2	source					785:790	a source	783:790	a source of oligosaccharides with potential functional and possible application for human nutrition	783:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	2	3	theme	interaction	359:369	arg1	chromatography					371:384	a hydrophilic interaction chromatography	345:384	a hydrophilic interaction chromatography	345:384	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	3	4	from	oligosaccharides	583:598	arg1	samples					607:613	the samples	603:613	the samples	603:613	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	4	5	theme	final	618:622	arg1	product					624:630	A final product	616:630	A final product with recovery of 63-96% of oligosaccharides	616:674	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	5	6	with	oligosaccharides	795:810	arg1	application					851:861	potential functional and possible application	817:861	potential functional and possible application for human nutrition	817:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	1	7	dep	characterization	131:146	arg1	the					127:129	the	127:129	the	127:129	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	2	8	theme	chromatography	371:384	arg1	Spectrometry					462:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	4	9	theme	oligosaccharides	659:674	arg1	oligosaccharides					659:674	oligosaccharides	659:674	oligosaccharides	659:674	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	4	9	theme	oligosaccharides	659:674	arg1	%					654:654	63-96%	649:654	63-96% of oligosaccharides	649:674	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	1	10	theme	goat	207:210	arg1	whey					219:222	goat cheese whey	207:222	goat cheese whey obtained from two types of goat milk	207:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	1	11	theme	present	196:202	arg1	oligosaccharides					169:184	oligosaccharides	169:184	oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk	169:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	3	12	theme	different	573:581	arg1	oligosaccharides					583:598	five different oligosaccharides	568:598	five different oligosaccharides in the samples	568:613	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	3	13	theme	Quadrupole	478:487	arg1	method					528:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	5	14	theme	possible	842:849	arg1	application					851:861	potential functional and possible application	817:861	potential functional and possible application for human nutrition	817:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	2	15	theme	filtration	322:331	arg1	process					333:339	a two-step cross-flow filtration process	300:339	a two-step cross-flow filtration process	300:339	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	2	16	theme	Chromatography	413:426	arg1	Spectrometry					462:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	5	17	dep	potential	817:825	arg1	functional					827:836	functional	827:836	functional	827:836	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	2	18	theme	cross-flow	311:320	arg1	process					333:339	a two-step cross-flow filtration process	300:339	a two-step cross-flow filtration process	300:339	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	1	19	theme	cheese	212:217	arg1	whey					219:222	goat cheese whey	207:222	goat cheese whey obtained from two types of goat milk	207:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	4	20	with	product	624:630	arg1	recovery					637:644	recovery	637:644	recovery of 63-96% of oligosaccharides	637:674	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	2	21	theme	two-step	302:309	arg1	process					333:339	a two-step cross-flow filtration process	300:339	a two-step cross-flow filtration process	300:339	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	2	22	theme	Mass	457:460	arg1	Spectrometry					462:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry	345:473	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	1	23	theme	present	91:97	arg1	research					99:106	The present research	87:106	The present research aimed	87:112	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	3	24	theme	UPLC-HDMS-Q-TOF	511:525	arg1	method					528:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	0	25	theme	cheese	34:39	arg1	whey					41:44	goat cheese whey	29:44	goat cheese whey	29:44	Composition and isolation of goat cheese whey oligosaccharides by membrane technology.
31356940	4	26	theme	original	712:719	arg1	whey					721:724	the original whey	708:724	the original whey	708:724	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	5	27	theme	goat	758:761	arg1	source					785:790	a source	783:790	a source of oligosaccharides with potential functional and possible application for human nutrition	783:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	5	27	theme	goat	758:761	arg1	whey					763:766	goat whey	758:766	goat whey	758:766	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	1	28	attach	present	196:202	arg2	oligosaccharides					169:184	oligosaccharides	169:184	oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk	169:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	1	28	attach	present	196:202	arg1	whey					219:222	goat cheese whey	207:222	goat cheese whey obtained from two types of goat milk	207:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	2	29	theme	Liquid	406:411	arg1	Chromatography					413:426	Ultra-Performance Liquid Chromatography	388:426	Ultra-Performance Liquid Chromatography	388:426	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	2	30	theme	High	441:444	arg1	Definition					446:455	a High Definition	439:455	a High Definition	439:455	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	0	31	theme	goat	29:32	arg1	whey					41:44	goat cheese whey	29:44	goat cheese whey	29:44	Composition and isolation of goat cheese whey oligosaccharides by membrane technology.
31356940	5	32	theme	potential	817:825	arg1	application					851:861	potential functional and possible application	817:861	potential functional and possible application for human nutrition	817:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	5	33	theme	human	867:871	arg1	nutrition					873:881	human nutrition	867:881	human nutrition	867:881	These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.
31356940	3	34	used	used	539:542	arg2	method					528:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	2	35	theme	hydrophilic	347:357	arg1	chromatography					371:384	a hydrophilic interaction chromatography	345:384	a hydrophilic interaction chromatography	345:384	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	0	36	theme	whey	41:44	arg1	isolation					16:24	isolation	16:24	isolation	16:24	Composition and isolation of goat cheese whey oligosaccharides by membrane technology.
31356940	0	36	theme	whey	41:44	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and isolation of goat cheese whey oligosaccharides by membrane technology.
31356940	1	37	from	whey	219:222	arg1	present					196:202	present	196:202	present	196:202	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	2	38	theme	cheese	271:276	arg1	whey					278:281	The goat cheese whey	262:281	The goat cheese whey	262:281	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	2	39	theme	goat	266:269	arg1	whey					278:281	The goat cheese whey	262:281	The goat cheese whey	262:281	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	0	40	theme	membrane	66:73	arg1	technology					75:84	membrane technology	66:84	membrane technology	66:84	Composition and isolation of goat cheese whey oligosaccharides by membrane technology.
31356940	3	41	theme	Time-of-Flight	489:502	arg1	method					528:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	3	42	theme	HILIC	505:509	arg1	method					528:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method	476:533	A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples.
31356940	2	43	theme	Ultra-Performance	388:404	arg1	Chromatography					413:426	Ultra-Performance Liquid Chromatography	388:426	Ultra-Performance Liquid Chromatography	388:426	The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry.
31356940	1	44	theme	goat	251:254	arg1	milk					256:259	goat milk	251:259	goat milk	251:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	4	45	theme	%	654:654	arg1	recovery					637:644	recovery	637:644	recovery of 63-96% of oligosaccharides	637:674	A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey.
31356940	1	46	from	present	196:202	arg1	whey					219:222	goat cheese whey	207:222	goat cheese whey obtained from two types of goat milk	207:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31356940	1	47	theme	milk	256:259	arg1	types					242:246	two types	238:246	two types of goat milk	238:259	The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk.
31401269	8	0	theme	MCS/ZnO	940:946	arg1	Alg					948:950	MCS/ZnO@Alg	940:950	MCS/ZnO@Alg	940:950	In addition, MCS/ZnO@Alg exhibited good recyclability and high sustainability.
31401269	6	1	theme	As	758:759	arg1	capability					775:784	As(V) adsorption capability	758:784	As(V) adsorption capability	758:784	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	7	2	theme	As	847:848	arg1	63.69 mg/g					915:924	63.69 mg/g	915:924	63.69 mg/g	915:924	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	7	2	theme	As	847:848	arg1	capacity					864:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	8	3	theme	@	947:947	arg1	Alg					948:950	MCS/ZnO@Alg	940:950	MCS/ZnO@Alg	940:950	In addition, MCS/ZnO@Alg exhibited good recyclability and high sustainability.
31401269	9	4	theme	ideal	1061:1065	arg1	candidate					1067:1075	an ideal candidate	1058:1075	an ideal candidate for addressing the grievous environmental threats caused by water pollution	1058:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	9	4	theme	ideal	1061:1065	arg1	beads					1048:1052	the MCS/ZnO@Alg gel beads	1028:1052	the MCS/ZnO@Alg gel beads	1028:1052	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	4	5	theme	MCS/ZnO	428:434	arg1	beads					444:448	MCS/ZnO@Alg gel beads	428:448	MCS/ZnO@Alg gel beads	428:448	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	6	6	theme	adsorption	662:671	arg1	experiments					673:683	The adsorption experiments	658:683	The adsorption experiments	658:683	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	9	7	theme	@	1039:1039	arg1	candidate					1067:1075	an ideal candidate	1058:1075	an ideal candidate for addressing the grievous environmental threats caused by water pollution	1058:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	9	7	theme	@	1039:1039	arg1	beads					1048:1052	the MCS/ZnO@Alg gel beads	1028:1052	the MCS/ZnO@Alg gel beads	1028:1052	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	2	8	theme	zinc	244:247	arg1	ZnO					256:258	ZnO	256:258	ZnO	256:258	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	2	8	theme	zinc	244:247	arg1	oxide					249:253	zinc oxide	244:253	zinc oxide (ZnO)	244:259	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	0	9	theme	As	87:88	arg1	adsorption					73:82	efficient adsorption	63:82	efficient adsorption of As(V)	63:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	6	10	theme	high	739:742	arg1	stability					744:752	high stability	739:752	high stability	739:752	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	6	11	theme	chemical	814:821	arg1	adsorption					823:832	chemical adsorption	814:832	chemical adsorption	814:832	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	8	12	theme	good	962:965	arg1	recyclability					967:979	good recyclability	962:979	good recyclability	962:979	In addition, MCS/ZnO@Alg exhibited good recyclability and high sustainability.
31401269	7	13	dep	63.69 mg/g	915:924	arg1	determined					876:885	determined	876:885	determined from the Langmuir model	876:909	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	9	14	theme	grievous	1096:1103	arg1	threats					1119:1125	the grievous environmental threats	1092:1125	the grievous environmental threats caused by water pollution	1092:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	7	15	theme	Langmuir	896:903	arg1	model					905:909	the Langmuir model	892:909	the Langmuir model	892:909	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	9	16	theme	MCS/ZnO	1032:1038	arg1	candidate					1067:1075	an ideal candidate	1058:1075	an ideal candidate for addressing the grievous environmental threats caused by water pollution	1058:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	9	16	theme	MCS/ZnO	1032:1038	arg1	beads					1048:1052	the MCS/ZnO@Alg gel beads	1028:1052	the MCS/ZnO@Alg gel beads	1028:1052	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	6	17	theme	gel	724:726	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	5	18	theme	FTIR	620:623	arg1	spectroscopy					625:636	FTIR spectroscopy	620:636	FTIR spectroscopy	620:636	The MCS/ZnO@Alg beads were characterized by SEM, FTIR spectroscopy, XRD, VSM, and XPS.
31401269	4	19	theme	Ca2+	560:563	arg1	ions					565:568	Ca2+ ions	560:568	Ca2+ ions	560:568	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	9	20	theme	environmental	1105:1117	arg1	threats					1119:1125	the grievous environmental threats	1092:1125	the grievous environmental threats caused by water pollution	1092:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	0	21	theme	MCS/ZnO	30:36	arg1	microspheres					46:57	chitosan-based MCS/ZnO@Alg gel microspheres	15:57	chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V)	15:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	5	22	theme	@	582:582	arg1	beads					587:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads were characterized by SEM, FTIR spectroscopy, XRD, VSM, and XPS.
31401269	4	23	from	material	532:539	arg1	presence					548:555	the presence	544:555	the presence of Ca2+ ions	544:568	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	4	24	theme	composite	522:530	arg1	material					532:539	the composite material	518:539	the composite material in the presence of Ca2+ ions	518:568	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	0	25	theme	chitosan-based	15:28	arg1	microspheres					46:57	chitosan-based MCS/ZnO@Alg gel microspheres	15:57	chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V)	15:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	5	26	theme	Alg	583:585	arg1	beads					587:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads were characterized by SEM, FTIR spectroscopy, XRD, VSM, and XPS.
31401269	6	27	theme	magnetic	715:722	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	0	28	theme	Alg	38:40	arg1	microspheres					46:57	chitosan-based MCS/ZnO@Alg gel microspheres	15:57	chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V)	15:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	9	29	theme	water	1137:1141	arg1	pollution					1143:1151	water pollution	1137:1151	water pollution	1137:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	6	30	theme	Alg	711:713	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	8	31	theme	high	985:988	arg1	sustainability					990:1003	high sustainability	985:1003	high sustainability	985:1003	In addition, MCS/ZnO@Alg exhibited good recyclability and high sustainability.
31401269	6	32	contain	have	734:737	arg2	capability					775:784	As(V) adsorption capability	758:784	As(V) adsorption capability	758:784	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	6	32	contain	have	734:737	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	6	32	contain	have	734:737	arg2	stability					744:752	high stability	739:752	high stability	739:752	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	0	33	theme	@	37:37	arg1	microspheres					46:57	chitosan-based MCS/ZnO@Alg gel microspheres	15:57	chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V)	15:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	4	34	theme	Alg	436:438	arg1	beads					444:448	MCS/ZnO@Alg gel beads	428:448	MCS/ZnO@Alg gel beads	428:448	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	7	35	theme	adsorption	853:862	arg1	63.69 mg/g					915:924	63.69 mg/g	915:924	63.69 mg/g	915:924	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	7	35	theme	adsorption	853:862	arg1	capacity					864:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	6	36	theme	@	710:710	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	2	37	theme	adsorbent	195:203	arg1	composite					185:193	a novel composite adsorbent	177:203	a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg)	177:286	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	0	38	theme	microspheres	46:57	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).	0:92	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	4	39	theme	@	435:435	arg1	beads					444:448	MCS/ZnO@Alg gel beads	428:448	MCS/ZnO@Alg gel beads	428:448	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	6	40	theme	MCS/ZnO	703:709	arg1	beads					728:732	the MCS/ZnO@Alg magnetic gel beads	699:732	the MCS/ZnO@Alg magnetic gel beads	699:732	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	0	41	theme	gel	42:44	arg1	microspheres					46:57	chitosan-based MCS/ZnO@Alg gel microspheres	15:57	chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V)	15:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	7	42	theme	V	850:850	arg1	63.69 mg/g					915:924	63.69 mg/g	915:924	63.69 mg/g	915:924	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	7	42	theme	V	850:850	arg1	capacity					864:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	9	43	theme	Alg	1040:1042	arg1	candidate					1067:1075	an ideal candidate	1058:1075	an ideal candidate for addressing the grievous environmental threats caused by water pollution	1058:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	9	43	theme	Alg	1040:1042	arg1	beads					1048:1052	the MCS/ZnO@Alg gel beads	1028:1052	the MCS/ZnO@Alg gel beads	1028:1052	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	2	44	theme	novel	179:183	arg1	composite					185:193	a novel composite adsorbent	177:203	a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg)	177:286	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	2	45	theme	sodium	266:271	arg1	Alg					283:285	Alg	283:285	Alg	283:285	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	2	45	theme	sodium	266:271	arg1	alginate					273:280	sodium alginate	266:280	sodium alginate (Alg)	266:286	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	4	46	theme	gel	440:442	arg1	beads					444:448	MCS/ZnO@Alg gel beads	428:448	MCS/ZnO@Alg gel beads	428:448	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	7	47	theme	maximum	839:845	arg1	63.69 mg/g					915:924	63.69 mg/g	915:924	63.69 mg/g	915:924	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	7	47	theme	maximum	839:845	arg1	capacity					864:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity	835:871	The maximum As(V) adsorption capacity as determined from the Langmuir model was 63.69 mg/g.
31401269	9	48	theme	gel	1044:1046	arg1	candidate					1067:1075	an ideal candidate	1058:1075	an ideal candidate for addressing the grievous environmental threats caused by water pollution	1058:1151	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	9	48	theme	gel	1044:1046	arg1	beads					1048:1052	the MCS/ZnO@Alg gel beads	1028:1052	the MCS/ZnO@Alg gel beads	1028:1052	This work proves that the MCS/ZnO@Alg gel beads are an ideal candidate for addressing the grievous environmental threats caused by water pollution.
31401269	3	49	theme	Fe3O4	382:386	arg1	nanoparticles					388:400	Fe3O4 nanoparticles	382:400	Fe3O4 nanoparticles	382:400	First, chitosan was coated on the surface of Fe3O4 nanoparticles by coprecipitation.
31401269	5	50	theme	MCS/ZnO	575:581	arg1	beads					587:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads	571:591	The MCS/ZnO@Alg beads were characterized by SEM, FTIR spectroscopy, XRD, VSM, and XPS.
31401269	2	51	from	groundwater	324:334	arg1	arsenic					311:317	arsenic	311:317	arsenic from groundwater	311:334	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	3	52	theme	nanoparticles	388:400	arg1	surface					371:377	the surface	367:377	the surface of Fe3O4 nanoparticles by coprecipitation	367:419	First, chitosan was coated on the surface of Fe3O4 nanoparticles by coprecipitation.
31401269	6	53	dep	capability	775:784	arg1	V					761:761	V	761:761	V	761:761	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
31401269	0	54	theme	efficient	63:71	arg1	adsorption					73:82	efficient adsorption	63:82	efficient adsorption of As(V)	63:91	Fabrication of chitosan-based MCS/ZnO@Alg gel microspheres for efficient adsorption of As(V).
31401269	2	55	theme	magnetic	219:226	arg1	MCS					238:240	MCS	238:240	MCS	238:240	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	2	55	theme	magnetic	219:226	arg1	chitosan					228:235	magnetic chitosan	219:235	magnetic chitosan (MCS)	219:241	Therefore, in this work, a novel composite adsorbent consisting of magnetic chitosan (MCS), zinc oxide (ZnO), and sodium alginate (Alg) was prepared to remove arsenic from groundwater.
31401269	4	56	with	MCS	479:481	arg1	ZnO					488:490	ZnO	488:490	ZnO	488:490	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	4	56	with	MCS	479:481	arg1	Alg					496:498	Alg	496:498	Alg	496:498	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	4	57	theme	ions	565:568	arg1	presence					548:555	the presence	544:555	the presence of Ca2+ ions	544:568	Then, MCS/ZnO@Alg gel beads were fabricated by combining MCS with ZnO and Alg, and crosslinking the composite material in the presence of Ca2+ ions.
31401269	6	58	theme	adsorption	764:773	arg1	capability					775:784	As(V) adsorption capability	758:784	As(V) adsorption capability	758:784	The adsorption experiments revealed that the MCS/ZnO@Alg magnetic gel beads have high stability and As(V) adsorption capability, and adsorbed As(V) through chemical adsorption.
29778066	4	0	theme	transport	886:894	arg1	hindrance					858:866	steric hindrance	851:866	steric hindrance	851:866	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	0	theme	transport	886:894	arg1	mechanism					906:914	the main TrOCs transport -limiting mechanism	871:914	the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	871:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	1	1	theme	recent	285:290	arg1	developments					292:303	the recent developments	281:303	the recent developments in FO membranes	281:319	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	5	2	theme	membrane	1255:1262	arg1	pores					1264:1268	the membrane pores	1251:1268	"shrinking" the membrane pores	1239:1268	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	3	3	theme	draw	795:798	arg1	solution					800:807	a draw solution	793:807	a draw solution	793:807	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	1	4	theme	trace	210:214	arg1	TrOCs					235:239	TrOCs	235:239	TrOCs	235:239	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	4	theme	trace	210:214	arg1	compounds					224:232	trace organic compounds	210:232	trace organic compounds (TrOCs)	210:240	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	4	5	theme	TrOCs	880:884	arg1	hindrance					858:866	steric hindrance	851:866	steric hindrance	851:866	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	5	theme	TrOCs	880:884	arg1	mechanism					906:914	the main TrOCs transport -limiting mechanism	871:914	the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	871:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	2	6	theme	water	504:508	arg1	permeability					510:521	greater water permeability	496:521	greater water permeability	496:521	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	1	7	theme	reverse	365:371	arg1	flux					378:381	reverse salt flux	365:381	reverse salt flux	365:381	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	2	8	with	membranes	481:489	arg1	selectivity					527:537	selectivity	527:537	selectivity	527:537	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	2	8	with	membranes	481:489	arg1	permeability					510:521	greater water permeability	496:521	greater water permeability	496:521	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	1	9	theme	organic	216:222	arg1	TrOCs					235:239	TrOCs	235:239	TrOCs	235:239	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	9	theme	organic	216:222	arg1	compounds					224:232	trace organic compounds	210:232	trace organic compounds (TrOCs)	210:240	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	0	10	theme	membrane	86:93	arg1	properties					95:104	membrane properties	86:104	membrane properties	86:104	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	1	11	from	wastewater	260:269	arg1	removal					199:205	the removal	195:205	the removal of trace organic compounds (TrOCs) from contaminated wastewater	195:269	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	12	theme	salt	373:376	arg1	flux					378:381	reverse salt flux	365:381	reverse salt flux	365:381	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	5	13	dep	"	1286:1286	arg1	charge					1309:1314	the negative surface charge	1288:1314	"shielding" the negative surface charge	1276:1314	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	1	14	theme	compounds	224:232	arg1	removal					199:205	the removal	195:205	the removal of trace organic compounds (TrOCs) from contaminated wastewater	195:269	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	5	15	theme	shrinking	1240:1248	arg1	"					1249:1249	"shrinking" the membrane pores	1239:1268	"shrinking" the membrane pores	1239:1268	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	1	16	theme	FO	308:309	arg1	membranes					311:319	FO membranes	308:319	FO membranes	308:319	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	6	17	theme	high	1416:1419	arg1	fluxes					1427:1432	high water fluxes	1416:1432	high water fluxes	1416:1432	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	1	18	theme	flux	378:381	arg1	effect					326:331	the effect	322:331	the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs	322:407	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	3	19	theme	several	810:816	arg1	conclusions					818:828	several conclusions	810:828	several conclusions	810:828	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	1	20	from	effect	326:331	arg1	rejection					390:398	the rejection	386:398	the rejection of TrOCs	386:407	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	6	21	theme	reverse	1447:1453	arg1	flux					1462:1465	the reverse solute flux	1443:1465	the reverse solute flux	1443:1465	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	2	22	theme	benchmark	548:556	arg1	membrane					586:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	6	23	theme	TrOCs	1508:1512	arg1	transport					1495:1503	the transport	1491:1503	the transport of TrOCs through the membrane	1491:1533	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	5	24	theme	TrOCs	1230:1234	arg1	rejection					1217:1225	the rejection	1213:1225	the rejection of TrOCs	1213:1234	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	2	25	theme	cellulose	558:566	arg1	membrane					586:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	0	26	theme	properties	95:104	arg1	solution					119:126	the draw solution	110:126	the draw solution	110:126	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	0	26	theme	properties	95:104	arg1	Role					78:81	Role	78:81	Role of membrane properties	78:104	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	5	27	theme	shielding	1277:1285	arg1	"					1286:1286	"shielding" the negative surface charge	1276:1314	"shielding" the negative surface charge	1276:1314	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	4	28	theme	-limiting	896:904	arg1	hindrance					858:866	steric hindrance	851:866	steric hindrance	851:866	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	28	theme	-limiting	896:904	arg1	mechanism					906:914	the main TrOCs transport -limiting mechanism	871:914	the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	871:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	3	29	theme	solution	800:807	arg1	absence					769:775	absence	769:775	absence	769:775	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	3	29	theme	solution	800:807	arg1	presence					781:788	presence	781:788	presence	781:788	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	5	30	theme	ionic	1102:1106	arg1	strength					1108:1115	ionic strength	1102:1115	ionic strength	1102:1115	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	6	31	theme	FO	1332:1333	arg1	operation					1335:1343	FO operation	1332:1343	FO operation	1332:1343	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	6	32	theme	water	1421:1425	arg1	fluxes					1427:1432	high water fluxes	1416:1432	high water fluxes	1416:1432	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	3	33	dep	absence	769:775	arg1	the					765:767	the	765:767	the	765:767	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	5	34	from	increase	1090:1097	arg1	strength					1108:1115	ionic strength	1102:1115	ionic strength	1102:1115	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	4	35	theme	TFC	924:926	arg1	membranes					928:936	TFC membranes	924:936	TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	924:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	2	36	from	bench-scale	612:622	arg1	terms					627:631	terms	627:631	terms of TrOCs permeability	627:653	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	0	37	theme	organic	19:25	arg1	compounds					27:35	trace organic compounds	13:35	trace organic compounds	13:35	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	5	38	theme	negative	1292:1299	arg1	charge					1309:1314	the negative surface charge	1288:1314	"shielding" the negative surface charge	1276:1314	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	2	39	theme	composite	465:473	arg1	TFC					476:478	TFC	476:478	TFC	476:478	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	2	39	theme	composite	465:473	arg1	membranes					481:489	two novel thin-film composite (TFC) membranes	445:489	two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane	445:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	1	40	theme	TrOCs	403:407	arg1	rejection					390:398	the rejection	386:398	the rejection of TrOCs	386:407	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	5	41	theme	surface	1301:1307	arg1	charge					1309:1314	the negative surface charge	1288:1314	"shielding" the negative surface charge	1276:1314	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	0	42	theme	trace	13:17	arg1	compounds					27:35	trace organic compounds	13:35	trace organic compounds	13:35	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	2	43	theme	thin-film	455:463	arg1	TFC					476:478	TFC	476:478	TFC	476:478	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	2	43	theme	thin-film	455:463	arg1	membranes					481:489	two novel thin-film composite (TFC) membranes	445:489	two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane	445:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	5	44	dep	"	1249:1249	arg1	pores					1264:1268	the membrane pores	1251:1268	"shrinking" the membrane pores	1239:1268	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	1	45	from	developments	292:303	arg1	membranes					311:319	FO membranes	308:319	FO membranes	308:319	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	46	theme	contaminated	247:258	arg1	wastewater					260:269	contaminated wastewater	247:269	contaminated wastewater	247:269	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	2	47	theme	novel	449:453	arg1	thin-film					455:463	novel thin-film	449:463	two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane	445:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	4	48	theme	electrostatic	1013:1025	arg1	interactions					1027:1038	electrostatic interactions	1013:1038	electrostatic interactions	1013:1038	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	1	49	theme	promising	170:178	arg1	osmosis					137:143	Forward osmosis	129:143	Forward osmosis (FO)	129:148	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	49	theme	promising	170:178	arg1	technology					180:189	a very promising technology	163:189	a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater	163:269	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	0	50	theme	compounds	27:35	arg1	Transport					0:8	Transport	0:8	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.	0:127	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	3	51	from	membranes	752:760	arg1	absence					769:775	absence	769:775	absence	769:775	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	3	51	from	membranes	752:760	arg1	presence					781:788	presence	781:788	presence	781:788	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	6	52	theme	solute	1455:1460	arg1	flux					1462:1465	the reverse solute flux	1443:1465	the reverse solute flux	1443:1465	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	4	53	theme	negative	949:956	arg1	charge					975:980	the negative membrane surface charge	945:980	the negative membrane surface charge	945:980	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	53	theme	negative	949:956	arg1	significant					985:995	significant	985:995	significant	985:995	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	54	from	case	1007:1010	arg1	membranes					928:936	TFC membranes	924:936	TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	924:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	0	55	theme	forward	51:57	arg1	membranes					67:75	novel forward osmosis membranes	45:75	novel forward osmosis membranes	45:75	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	4	56	theme	steric	851:856	arg1	hindrance					858:866	steric hindrance	851:866	steric hindrance	851:866	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	56	theme	steric	851:856	arg1	mechanism					906:914	the main TrOCs transport -limiting mechanism	871:914	the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	871:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	6	57	contain	has	1467:1469	arg1	flux					1462:1465	the reverse solute flux	1443:1465	the reverse solute flux	1443:1465	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	6	57	contain	has	1467:1469	arg2	impact					1481:1486	no direct impact	1471:1486	no direct impact	1471:1486	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	0	58	theme	novel	45:49	arg1	membranes					67:75	novel forward osmosis membranes	45:75	novel forward osmosis membranes	45:75	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	3	59	theme	solute-membrane	671:685	arg1	interactions					687:698	the solute-membrane interactions	667:698	the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution	667:807	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	1	60	theme	higher	343:348	arg1	flux					356:359	a higher water flux	341:359	a higher water flux	341:359	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	5	61	dep	the	1128:1130	arg1	draw					1132:1135	draw	1132:1135	draw solution in the vicinity of	1132:1163	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	5	61	dep	the	1128:1130	arg1	inside					1177:1182	inside	1177:1182	inside the membrane	1177:1195	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	4	62	theme	membrane	958:965	arg1	charge					975:980	the negative membrane surface charge	945:980	the negative membrane surface charge	945:980	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	62	theme	membrane	958:965	arg1	significant					985:995	significant	985:995	significant	985:995	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	2	63	theme	greater	496:502	arg1	permeability					510:521	greater water permeability	496:521	greater water permeability	496:521	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	2	64	theme	CTA	581:583	arg1	membrane					586:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	1	65	theme	water	350:354	arg1	flux					356:359	a higher water flux	341:359	a higher water flux	341:359	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	2	66	theme	tri-acetate	568:578	arg1	membrane					586:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	the benchmark cellulose tri-acetate (CTA) membrane	544:593	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	6	67	theme	concentration	1353:1365	arg1	polarisation					1367:1378	solute concentration polarisation	1346:1378	solute concentration polarisation	1346:1378	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	5	68	from	solution	1137:1144	arg1	vicinity					1153:1160	the vicinity	1149:1160	the vicinity of	1149:1163	Secondly, the increase in ionic strength induced by the draw solution in the vicinity of and perhaps inside the membrane seems to favour the rejection of TrOCs by "shrinking" the membrane pores or by "shielding" the negative surface charge.
29778066	1	69	theme	flux	356:359	arg1	effect					326:331	the effect	322:331	the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs	322:407	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	2	70	theme	permeability	642:653	arg1	terms					627:631	terms	627:631	terms of TrOCs permeability	627:653	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	4	71	theme	steric	1058:1063	arg1	hindrance					1065:1073	steric hindrance	1058:1073	steric hindrance	1058:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	6	72	theme	solute	1346:1351	arg1	polarisation					1367:1378	solute concentration polarisation	1346:1378	solute concentration polarisation	1346:1378	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	3	73	theme	TrOCs	730:734	arg1	transport					717:725	the transport	713:725	the transport of TrOCs	713:734	By probing the solute-membrane interactions that dictate the transport of TrOCs through the two membranes in the absence and presence of a draw solution, several conclusions were drawn.
29778066	4	74	theme	main	875:878	arg1	hindrance					858:866	steric hindrance	851:866	steric hindrance	851:866	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	74	theme	main	875:878	arg1	mechanism					906:914	the main TrOCs transport -limiting mechanism	871:914	the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance	871:1073	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	6	75	theme	direct	1474:1479	arg1	impact					1481:1486	no direct impact	1471:1486	no direct impact	1471:1486	Lastly, during FO operation, solute concentration polarisation becomes detrimental when working at high water fluxes, whereas the reverse solute flux has no direct impact on the transport of TrOCs through the membrane.
29778066	2	76	theme	TrOCs	636:640	arg1	permeability					642:653	TrOCs permeability	636:653	TrOCs permeability	636:653	In this study two novel thin-film composite (TFC) membranes with greater water permeability and selectivity than the benchmark cellulose tri-acetate (CTA) membrane were compared at bench-scale in terms of TrOCs permeability.
29778066	1	77	theme	Forward	129:135	arg1	osmosis					137:143	Forward osmosis	129:143	Forward osmosis (FO)	129:148	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	77	theme	Forward	129:135	arg1	technology					180:189	a very promising technology	163:189	a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater	163:269	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	1	77	theme	Forward	129:135	arg1	FO					146:147	FO	146:147	FO	146:147	Forward osmosis (FO) offers to be a very promising technology for the removal of trace organic compounds (TrOCs) from contaminated wastewater, and with the recent developments in FO membranes, the effect of both a higher water flux and reverse salt flux on the rejection of TrOCs needs to be explored.
29778066	0	78	theme	osmosis	59:65	arg1	membranes					67:75	novel forward osmosis membranes	45:75	novel forward osmosis membranes	45:75	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	0	79	dep	Transport	0:8	arg1	solution					119:126	the draw solution	110:126	the draw solution	110:126	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	0	79	dep	Transport	0:8	arg1	Role					78:81	Role	78:81	Role of membrane properties	78:104	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	4	80	dep	dominate	1044:1051	arg1	charge					975:980	the negative membrane surface charge	945:980	the negative membrane surface charge	945:980	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	80	dep	dominate	1044:1051	arg1	case					1007:1010	case	1007:1010	case	1007:1010	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	80	dep	dominate	1044:1051	arg1	significant					985:995	significant	985:995	significant	985:995	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	0	81	dep	solution	119:126	arg1	draw					114:117	draw	114:117	draw	114:117	Transport of trace organic compounds through novel forward osmosis membranes: Role of membrane properties and the draw solution.
29778066	4	82	theme	surface	967:973	arg1	charge					975:980	the negative membrane surface charge	945:980	the negative membrane surface charge	945:980	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
29778066	4	82	theme	surface	967:973	arg1	significant					985:995	significant	985:995	significant	985:995	Firstly, steric hindrance is the main TrOCs transport -limiting mechanism through TFC membranes unless the negative membrane surface charge is significant, in which case, electrostatic interactions can dominate over steric hindrance.
30798163	4	0	theme	@	987:987	arg1	CS-ED					988:992	CNC-ED@CS-ED	981:992	CNC-ED@CS-ED	981:992	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	1	theme	Fourier-transform	673:689	arg1	spectrometry					700:711	Fourier-transform infrared spectrometry	673:711	Fourier-transform infrared spectrometry	673:711	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	4	2	theme	adsorption	1040:1049	arg1	444.44 mg/g					1096:1106	444.44 mg/g	1096:1106	444.44 mg/g	1096:1106	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	2	theme	adsorption	1040:1049	arg1	capacity					1051:1058	the maximum adsorption capacity	1028:1058	the maximum adsorption capacity obtained from the Langmuir model	1028:1091	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	3	theme	CNC-ED	981:986	arg1	CS-ED					988:992	CNC-ED@CS-ED	981:992	CNC-ED@CS-ED	981:992	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	4	theme	X-ray	653:657	arg1	spectroscopy					659:670	energy-dispersive X-ray spectroscopy	635:670	energy-dispersive X-ray spectroscopy	635:670	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	1	5	theme	human	172:176	arg1	health					178:183	human health	172:183	human health	172:183	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	2	6	from	chitosan	441:448	arg1	group					324:328	the carboxyl group	311:328	the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS)	311:453	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	6	from	chitosan	441:448	arg1	DS					333:334	DS	333:334	DS	333:334	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	3	7	dep	morphology	777:786	arg1	the					773:775	the	773:775	the	773:775	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	0	8	from	Adsorption	0:9	arg1	cellulose					64:72	bilayer amino-functionalized cellulose	35:72	bilayer amino-functionalized cellulose	35:72	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose nanocrystals/chitosan composite.
30798163	5	9	theme	promising	1195:1203	arg1	material					1215:1222	a promising composite material	1193:1222	a promising composite material for the removal of DS from wastewater	1193:1260	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	5	9	theme	promising	1195:1203	arg1	it					1187:1188	it	1187:1188	it	1187:1188	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	5	10	from	wastewater	1251:1260	arg1	removal					1232:1238	the removal	1228:1238	the removal of DS from wastewater	1228:1260	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	5	11	theme	composite	1205:1213	arg1	material					1215:1222	a promising composite material	1193:1222	a promising composite material for the removal of DS from wastewater	1193:1260	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	5	11	theme	composite	1205:1213	arg1	it					1187:1188	it	1187:1188	it	1187:1188	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	4	12	theme	adsorption	959:968	arg1	process					970:976	the DS adsorption process	952:976	the DS adsorption process on CNC-ED@CS-ED	952:992	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	5	13	theme	DS	1243:1244	arg1	removal					1232:1238	the removal	1228:1238	the removal of DS from wastewater	1228:1260	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	4	14	theme	chemical	1003:1010	arg1	adsorption					1012:1021	chemical adsorption	1003:1021	chemical adsorption	1003:1021	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	15	theme	infrared	691:698	arg1	spectrometry					700:711	Fourier-transform infrared spectrometry	673:711	Fourier-transform infrared spectrometry	673:711	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	1	16	theme	Residual	107:114	arg1	sodium					127:132	Residual diclofenac sodium	107:132	Residual diclofenac sodium (DS) in the environment	107:156	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	1	16	theme	Residual	107:114	arg1	DS					135:136	DS	135:136	DS	135:136	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	2	17	theme	cellulose	408:416	arg1	CNC					432:434	CNC	432:434	CNC	432:434	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	17	theme	cellulose	408:416	arg1	nanocrystals					418:429	cellulose nanocrystals	408:429	cellulose nanocrystals (CNC)	408:435	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	5	18	theme	@	1115:1115	arg1	CS-ED					1116:1120	CNC-ED@CS-ED	1109:1120	CNC-ED@CS-ED	1109:1120	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	1	19	theme	diclofenac	116:125	arg1	sodium					127:132	Residual diclofenac sodium	107:132	Residual diclofenac sodium (DS) in the environment	107:156	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	1	19	theme	diclofenac	116:125	arg1	DS					135:136	DS	135:136	DS	135:136	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	5	20	theme	good	1131:1134	arg1	capacity					1147:1154	good adsorption capacity	1131:1154	good adsorption capacity	1131:1154	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	5	21	theme	adsorption	1136:1145	arg1	capacity					1147:1154	good adsorption capacity	1131:1154	good adsorption capacity	1131:1154	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	2	22	theme	pH.	350:352	arg1	work					362:365	this work	357:365	a suitable pH. In this work	339:365	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	0	23	theme	sodium	25:30	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose	0:72	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose nanocrystals/chitosan composite.
30798163	2	24	theme	ionic	295:299	arg1	bond					301:304	an ionic bond	292:304	an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers	292:532	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	25	theme	adsorbent	386:394	arg1	composite					376:384	a novel composite adsorbent	368:394	a novel composite adsorbent composed of cellulose nanocrystals (CNC)	368:435	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	3	26	theme	X-ray	718:722	arg1	spectroscopy					738:749	X-ray photoelectron spectroscopy	718:749	X-ray photoelectron spectroscopy	718:749	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	2	27	theme	promising	188:196	arg1	use					227:229	the use	223:229	the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers	223:532	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	27	theme	promising	188:196	arg1	method					198:203	A promising method	186:203	A promising method for DS removal	186:218	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	28	theme	DS	333:334	arg1	group					324:328	the carboxyl group	311:328	the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS)	311:453	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	28	theme	DS	333:334	arg1	DS					333:334	DS	333:334	DS	333:334	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	4	29	theme	maximum	1032:1038	arg1	444.44 mg/g					1096:1106	444.44 mg/g	1096:1106	444.44 mg/g	1096:1106	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	29	theme	maximum	1032:1038	arg1	capacity					1051:1058	the maximum adsorption capacity	1028:1058	the maximum adsorption capacity obtained from the Langmuir model	1028:1091	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	2	30	theme	novel	370:374	arg1	composite					376:384	a novel composite adsorbent	368:394	a novel composite adsorbent composed of cellulose nanocrystals (CNC)	368:435	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	0	31	theme	bilayer	35:41	arg1	cellulose					64:72	bilayer amino-functionalized cellulose	35:72	bilayer amino-functionalized cellulose	35:72	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose nanocrystals/chitosan composite.
30798163	3	32	theme	photoelectron	724:736	arg1	spectroscopy					738:749	X-ray photoelectron spectroscopy	718:749	X-ray photoelectron spectroscopy	718:749	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	4	33	theme	mechanism	941:949	arg1	modeling					914:921	modeling	914:921	modeling of the adsorption mechanism	914:949	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	33	theme	mechanism	941:949	arg1	optimization					872:883	the optimization	868:883	the optimization of adsorption conditions	868:908	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	34	theme	transmission	601:612	arg1	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	35	theme	adsorbent	848:856	arg1	morphology					777:786	morphology	777:786	morphology	777:786	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	35	theme	adsorbent	848:856	arg1	mechanism					802:810	synthetic mechanism	792:810	synthetic mechanism	792:810	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	0	36	theme	diclofenac	14:23	arg1	sodium					25:30	diclofenac sodium	14:30	diclofenac sodium	14:30	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose nanocrystals/chitosan composite.
30798163	4	37	theme	adsorption	930:939	arg1	mechanism					941:949	the adsorption mechanism	926:949	the adsorption mechanism	926:949	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	38	theme	electron	580:587	arg1	microscopy					589:598	scanning electron microscopy	571:598	scanning electron microscopy	571:598	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	spectrometry					700:711	Fourier-transform infrared spectrometry	673:711	Fourier-transform infrared spectrometry	673:711	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	microscopy					589:598	scanning electron microscopy	571:598	scanning electron microscopy	571:598	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	spectroscopy					659:670	energy-dispersive X-ray spectroscopy	635:670	energy-dispersive X-ray spectroscopy	635:670	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	methods					552:558	Characterization methods	535:558	Characterization methods	535:558	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	spectroscopy					738:749	X-ray photoelectron spectroscopy	718:749	X-ray photoelectron spectroscopy	718:749	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	39	theme	Characterization	535:550	arg1	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	4	40	theme	DS	956:957	arg1	process					970:976	the DS adsorption process	952:976	the DS adsorption process on CNC-ED@CS-ED	952:992	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	41	theme	conditions	899:908	arg1	modeling					914:921	modeling	914:921	modeling of the adsorption mechanism	914:949	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	4	41	theme	conditions	899:908	arg1	optimization					872:883	the optimization	868:883	the optimization of adsorption conditions	868:908	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	5	42	theme	CNC-ED	1109:1114	arg1	CS-ED					1116:1120	CNC-ED@CS-ED	1109:1120	CNC-ED@CS-ED	1109:1120	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	0	43	theme	amino-functionalized	43:62	arg1	cellulose					64:72	bilayer amino-functionalized cellulose	35:72	bilayer amino-functionalized cellulose	35:72	Adsorption of diclofenac sodium on bilayer amino-functionalized cellulose nanocrystals/chitosan composite.
30798163	5	44	theme	high	1160:1163	arg1	sustainability					1165:1178	high sustainability	1160:1178	high sustainability	1160:1178	CNC-ED@CS-ED exhibits good adsorption capacity and high sustainability; thus, it is a promising composite material for the removal of DS from wastewater.
30798163	4	45	theme	adsorption	888:897	arg1	conditions					899:908	adsorption conditions	888:908	adsorption conditions	888:908	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	3	46	used	used	757:760	arg2	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	46	used	used	757:760	arg2	spectrometry					700:711	Fourier-transform infrared spectrometry	673:711	Fourier-transform infrared spectrometry	673:711	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	46	used	used	757:760	arg2	spectroscopy					738:749	X-ray photoelectron spectroscopy	718:749	X-ray photoelectron spectroscopy	718:749	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	46	used	used	757:760	arg2	microscopy					589:598	scanning electron microscopy	571:598	scanning electron microscopy	571:598	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	46	used	used	757:760	arg2	methods					552:558	Characterization methods	535:558	Characterization methods	535:558	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	46	used	used	757:760	arg2	spectroscopy					659:670	energy-dispersive X-ray spectroscopy	635:670	energy-dispersive X-ray spectroscopy	635:670	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	47	theme	energy-dispersive	635:651	arg1	spectroscopy					659:670	energy-dispersive X-ray spectroscopy	635:670	energy-dispersive X-ray spectroscopy	635:670	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	2	48	from	work	362:365	arg1	group					324:328	the carboxyl group	311:328	the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS)	311:453	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	48	from	work	362:365	arg1	DS					333:334	DS	333:334	DS	333:334	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	4	49	theme	Langmuir	1078:1085	arg1	model					1087:1091	the Langmuir model	1074:1091	the Langmuir model	1074:1091	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
30798163	2	50	theme	DS	209:210	arg1	removal					212:218	DS removal	209:218	DS removal	209:218	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	51	theme	amino	265:269	arg1	groups					271:276	amino groups	265:276	amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers	265:532	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	52	theme	carboxyl	315:322	arg1	group					324:328	the carboxyl group	311:328	the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS)	311:453	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	52	theme	carboxyl	315:322	arg1	DS					333:334	DS	333:334	DS	333:334	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	3	53	theme	electron	614:621	arg1	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	54	theme	scanning	571:578	arg1	microscopy					589:598	scanning electron microscopy	571:598	scanning electron microscopy	571:598	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	3	55	theme	synthetic	792:800	arg1	mechanism					802:810	synthetic mechanism	792:810	synthetic mechanism	792:810	Characterization methods, including scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy, were used to confirm the morphology and synthetic mechanism of the double- amino-functionalized adsorbent.
30798163	2	56	dep	work	362:365	arg1	In					354:355	a suitable pH. In this work	339:365	a suitable pH. In this work	339:365	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	57	theme	suitable	341:348	arg1	work					362:365	this work	357:365	a suitable pH. In this work	339:365	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	1	58	from	sodium	127:132	arg1	environment					146:156	the environment	142:156	the environment	142:156	Residual diclofenac sodium (DS) in the environment is harmful to human health.
30798163	2	59	theme	adsorbents	234:243	arg1	use					227:229	the use	223:229	the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers	223:532	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	59	theme	adsorbents	234:243	arg1	method					198:203	A promising method	186:203	A promising method for DS removal	186:218	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	60	from	composite	376:384	arg1	group					324:328	the carboxyl group	311:328	the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS)	311:453	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	2	60	from	composite	376:384	arg1	DS					333:334	DS	333:334	DS	333:334	A promising method for DS removal is the use of adsorbents functionalized with amino groups that can form an ionic bond with the carboxyl group of DS at a suitable pH. In this work, a novel composite adsorbent composed of cellulose nanocrystals (CNC) and chitosan (CS) has been synthesized and functionalized by ethylenediamine (ED) in both layers.
30798163	4	61	from	process	970:976	arg1	CS-ED					988:992	CNC-ED@CS-ED	981:992	CNC-ED@CS-ED	981:992	Based on the optimization of adsorption conditions and modeling of the adsorption mechanism, the DS adsorption process on CNC-ED@CS-ED involves chemical adsorption, and the maximum adsorption capacity obtained from the Langmuir model is 444.44 mg/g.
31078600	0	0	theme	delivery	101:108	arg1	carrier					110:116	dual delivery carrier	96:116	dual delivery carrier	96:116	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	5	1	theme	drug	772:775	arg1	release					777:783	In vitro drug release	763:783	In vitro drug release	763:783	In vitro drug release displayed an accelerated release under weak acidic or reducing media.
31078600	6	2	theme	cell	885:888	arg1	apoptosis					890:898	cell apoptosis	885:898	cell apoptosis	885:898	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	0	3	theme	dual	96:99	arg1	carrier					110:116	dual delivery carrier	96:116	dual delivery carrier	96:116	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	6	4	theme	drug-loaded	919:929	arg1	film					941:944	the drug-loaded composite film	915:944	the drug-loaded composite film	915:944	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	2	5	theme	Dual	369:372	arg1	5-Fluorouracil					396:409	5-Fluorouracil	396:409	5-Fluorouracil (5-FU)	396:416	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	5	theme	Dual	369:372	arg1	drugs					374:378	Dual drugs	369:378	Dual drugs	369:378	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	5	theme	Dual	369:372	arg1	anticancer					384:393	an anticancer	381:393	an anticancer	381:393	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	5	theme	Dual	369:372	arg1	drugs					488:492	model drugs	482:492	model drugs	482:492	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	5	theme	Dual	369:372	arg1	antibiotic					425:434	antibiotic	425:434	antibiotic	425:434	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	8	6	contain	has	1190:1192	arg1	system					1183:1188	our simple and effective dual drug-loaded system	1141:1188	our simple and effective dual drug-loaded system	1141:1188	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	8	6	contain	has	1190:1192	arg2	potential					1198:1206	the potential to be used for cancer therapy in the future	1194:1250	the potential to be used for cancer therapy in the future	1194:1250	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	6	7	theme	glutathione	1003:1013	arg1	presence					991:998	presence	991:998	presence of glutathione (GSH)	991:1019	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	8	8	theme	drug-loaded	1171:1181	arg1	system					1183:1188	our simple and effective dual drug-loaded system	1141:1188	our simple and effective dual drug-loaded system	1141:1188	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	4	9	dep	microspheres	678:689	arg1	endowed					691:697	endowed	691:697	endowed	691:697	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	1	10	theme	di-sulphide	287:297	arg1	dihydrochloride					322:336	a di-sulphide cross-linker cystamine dihydrochloride	285:336	a di-sulphide cross-linker cystamine dihydrochloride (CYS)	285:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	1	10	theme	di-sulphide	287:297	arg1	CYS					339:341	CYS	339:341	CYS	339:341	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	8	11	from	therapy	1230:1236	arg1	future					1245:1250	future	1245:1250	future	1245:1250	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	8	12	theme	simple	1145:1150	arg1	system					1183:1188	our simple and effective dual drug-loaded system	1141:1188	our simple and effective dual drug-loaded system	1141:1188	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	3	13	theme	hydrogel	518:525	arg1	films					527:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	6	14	theme	apoptosis	890:898	arg1	studies					900:906	The in vitro cytotoxicity and cell apoptosis studies	855:906	The in vitro cytotoxicity and cell apoptosis studies	855:906	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	0	15	theme	Redox-responsive	0:15	arg1	films					32:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	1	16	theme	chitosan	208:215	arg1	microspheres					222:233	chitosan (CS) microspheres	208:233	chitosan (CS) microspheres	208:233	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	0	17	theme	blend	17:21	arg1	films					32:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	3	18	theme	composite	508:516	arg1	films					527:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	4	19	theme	dispersed	655:663	arg1	microspheres					678:689	the well dispersed spherical CS microspheres	646:689	the well dispersed spherical CS microspheres endowed hydrogel film	646:711	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	2	20	theme	Tetracycline	437:448	arg1	TH					465:466	TH	465:466	TH	465:466	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	20	theme	Tetracycline	437:448	arg1	antibiotic					425:434	antibiotic	425:434	antibiotic	425:434	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	20	theme	Tetracycline	437:448	arg1	Hydrochloride					450:462	Tetracycline Hydrochloride	437:462	Tetracycline Hydrochloride (TH)	437:467	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	1	21	theme	cross-linker	299:310	arg1	dihydrochloride					322:336	a di-sulphide cross-linker cystamine dihydrochloride	285:336	a di-sulphide cross-linker cystamine dihydrochloride (CYS)	285:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	1	21	theme	cross-linker	299:310	arg1	CYS					339:341	CYS	339:341	CYS	339:341	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	8	22	theme	dual	1166:1169	arg1	system					1183:1188	our simple and effective dual drug-loaded system	1141:1188	our simple and effective dual drug-loaded system	1141:1188	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	1	23	theme	cystamine	312:320	arg1	dihydrochloride					322:336	a di-sulphide cross-linker cystamine dihydrochloride	285:336	a di-sulphide cross-linker cystamine dihydrochloride (CYS)	285:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	1	23	theme	cystamine	312:320	arg1	CYS					339:341	CYS	339:341	CYS	339:341	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	5	24	theme	accelerated	798:808	arg1	release					810:816	an accelerated release	795:816	an accelerated release	795:816	In vitro drug release displayed an accelerated release under weak acidic or reducing media.
31078600	5	25	theme	reducing	839:846	arg1	media					848:852	reducing media	839:852	reducing media	839:852	In vitro drug release displayed an accelerated release under weak acidic or reducing media.
31078600	0	26	theme	hydrogel	23:30	arg1	films					32:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films	0:36	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	7	27	theme	excellent	1055:1063	arg1	efficacy					1079:1086	excellent antimicrobial efficacy	1055:1086	excellent antimicrobial efficacy against E.coli and Staphylococcus aureus	1055:1127	Moreover, the film has exhibited excellent antimicrobial efficacy against E.coli and Staphylococcus aureus.
31078600	1	28	theme	simple	121:126	arg1	method					128:133	A simple method	119:133	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS)	119:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	6	29	theme	composite	931:939	arg1	film					941:944	the drug-loaded composite film	915:944	the drug-loaded composite film	915:944	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	3	30	theme	prepared	499:506	arg1	films					527:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films	495:531	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	8	31	theme	cancer	1223:1228	arg1	therapy					1230:1236	cancer therapy	1223:1236	cancer therapy in the future	1223:1250	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	6	32	theme	HepG2	976:980	arg1	cells					982:986	HepG2 cells	976:986	HepG2 cells	976:986	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	5	33	dep	In	763:764	arg1	vitro					766:770	vitro	766:770	vitro	766:770	In vitro drug release displayed an accelerated release under weak acidic or reducing media.
31078600	6	34	theme	cytotoxicity	868:879	arg1	studies					900:906	The in vitro cytotoxicity and cell apoptosis studies	855:906	The in vitro cytotoxicity and cell apoptosis studies	855:906	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	4	35	theme	CS	675:676	arg1	microspheres					678:689	the well dispersed spherical CS microspheres	646:689	the well dispersed spherical CS microspheres endowed hydrogel film	646:711	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	7	36	theme	antimicrobial	1065:1077	arg1	efficacy					1079:1086	excellent antimicrobial efficacy	1055:1086	excellent antimicrobial efficacy against E.coli and Staphylococcus aureus	1055:1127	Moreover, the film has exhibited excellent antimicrobial efficacy against E.coli and Staphylococcus aureus.
31078600	8	37	theme	effective	1156:1164	arg1	system					1183:1188	our simple and effective dual drug-loaded system	1141:1188	our simple and effective dual drug-loaded system	1141:1188	Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
31078600	6	38	dep	showed	908:913	arg1	caused					946:951	caused	946:951	showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH)	908:1019	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	1	39	theme	carboxymethyl	240:252	arg1	hydrogel					270:277	carboxymethyl cellulose (CMC) hydrogel	240:277	carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS)	240:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	0	40	theme	cellulose/chitosan	61:78	arg1	microspheres					80:91	carboxymethyl cellulose/chitosan microspheres	47:91	carboxymethyl cellulose/chitosan microspheres as dual delivery carrier	47:116	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	6	41	dep	in	859:860	arg1	vitro					862:866	vitro	862:866	vitro	862:866	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	6	42	theme	in	859:860	arg1	cytotoxicity					868:879	in vitro cytotoxicity	859:879	in vitro cytotoxicity	859:879	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	0	43	theme	carboxymethyl	47:59	arg1	microspheres					80:91	carboxymethyl cellulose/chitosan microspheres	47:91	carboxymethyl cellulose/chitosan microspheres as dual delivery carrier	47:116	Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
31078600	6	44	theme	enhanced	953:960	arg1	inhibition					962:971	enhanced inhibition	953:971	enhanced inhibition to HepG2 cells	953:986	The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH).
31078600	2	45	used	used	474:477	arg2	drugs					488:492	model drugs	482:492	model drugs	482:492	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	anticancer					384:393	an anticancer	381:393	an anticancer	381:393	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	drugs					374:378	Dual drugs	369:378	Dual drugs	369:378	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	5-Fluorouracil					396:409	5-Fluorouracil	396:409	5-Fluorouracil (5-FU)	396:416	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	antibiotic					425:434	antibiotic	425:434	antibiotic	425:434	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	Hydrochloride					450:462	Tetracycline Hydrochloride	437:462	Tetracycline Hydrochloride (TH)	437:467	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	45	used	used	474:477	arg2	5-FU					412:415	5-FU	412:415	5-FU	412:415	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	3	46	theme	rheology	569:576	arg1	measurement					578:588	rheology measurement	569:588	rheology measurement	569:588	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	3	46	theme	rheology	569:576	arg1	FTIR					558:561	FTIR	558:561	FTIR	558:561	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	4	47	theme	hydrogel	699:706	arg1	film					708:711	hydrogel film	699:711	hydrogel film	699:711	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	3	48	theme	swelling	606:613	arg1	FTIR					558:561	FTIR	558:561	FTIR	558:561	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	3	48	theme	swelling	606:613	arg1	tests					615:619	mechanical and swelling tests	591:619	mechanical and swelling tests	591:619	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	4	49	theme	spherical	665:673	arg1	microspheres					678:689	the well dispersed spherical CS microspheres	646:689	the well dispersed spherical CS microspheres endowed hydrogel film	646:711	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	4	50	theme	mechanical	723:732	arg1	properties					734:743	mechanical properties	723:743	mechanical properties	723:743	Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability.
31078600	1	51	theme	cellulose	254:262	arg1	hydrogel					270:277	carboxymethyl cellulose (CMC) hydrogel	240:277	carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS)	240:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	1	52	theme	redox-responsive	148:163	arg1	films					184:188	redox-responsive composite hydrogel films	148:188	redox-responsive composite hydrogel films	148:188	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	5	53	theme	In	763:764	arg1	release					777:783	In vitro drug release	763:783	In vitro drug release	763:783	In vitro drug release displayed an accelerated release under weak acidic or reducing media.
31078600	3	54	theme	mechanical	591:600	arg1	FTIR					558:561	FTIR	558:561	FTIR	558:561	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	3	54	theme	mechanical	591:600	arg1	tests					615:619	mechanical and swelling tests	591:619	mechanical and swelling tests	591:619	The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests.
31078600	1	55	theme	composite	165:173	arg1	films					184:188	redox-responsive composite hydrogel films	148:188	redox-responsive composite hydrogel films	148:188	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	1	56	theme	CMC	265:267	arg1	hydrogel					270:277	carboxymethyl cellulose (CMC) hydrogel	240:277	carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS)	240:342	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
31078600	2	57	theme	model	482:486	arg1	5-Fluorouracil					396:409	5-Fluorouracil	396:409	5-Fluorouracil (5-FU)	396:416	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	57	theme	model	482:486	arg1	drugs					374:378	Dual drugs	369:378	Dual drugs	369:378	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	57	theme	model	482:486	arg1	anticancer					384:393	an anticancer	381:393	an anticancer	381:393	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	57	theme	model	482:486	arg1	drugs					488:492	model drugs	482:492	model drugs	482:492	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	2	57	theme	model	482:486	arg1	antibiotic					425:434	antibiotic	425:434	antibiotic	425:434	Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs.
31078600	1	58	theme	hydrogel	175:182	arg1	films					184:188	redox-responsive composite hydrogel films	148:188	redox-responsive composite hydrogel films	148:188	A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper.
29728997	11	0	with	Films	1154:1158	arg1	pullulan					1169:1176	26% pullulan	1165:1176	26% pullulan	1165:1176	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	11	0	with	Films	1154:1158	arg1	CNF					1192:1194	1% CNF	1189:1194	1% CNF	1189:1194	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	11	0	with	Films	1154:1158	arg1	HPMC					1183:1186	74% HPMC	1179:1186	74% HPMC	1179:1186	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	11	0	with	Films	1154:1158	arg1	glycerine					1204:1212	5% glycerine	1201:1212	5% glycerine	1201:1212	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	12	1	theme	captopril	1393:1401	arg1	tablet					1414:1419	a conventional captopril sublingual tablet	1378:1419	a conventional captopril sublingual tablet	1378:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	5	2	theme	design	645:650	arg1	percentages					581:591	The concentration percentages	563:591	The concentration percentages of pullulan and glycerine	563:617	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	5	2	theme	design	645:650	arg1	factors					652:658	the design factors	641:658	the design factors	641:658	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	7	3	dep	compatibility	829:841	arg1	the					825:827	the	825:827	the	825:827	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	1	4	theme	patient-friendly	148:163	arg1	film					196:199	a patient-friendly captopril fast-dissolving oral film	146:199	a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time	146:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	7	5	theme	HPMC	884:887	arg1	blend					889:893	HPMC blend	884:893	HPMC blend	884:893	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	12	6	theme	in	1329:1330	arg1	comparison					1337:1346	The in vivo comparison	1325:1346	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet	1325:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	13	7	dep	in	1610:1611	arg1	vivo					1613:1616	vivo	1613:1616	vivo	1613:1616	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	5	8	theme	pullulan	596:603	arg1	percentages					581:591	The concentration percentages	563:591	The concentration percentages of pullulan and glycerine	563:617	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	5	8	theme	pullulan	596:603	arg1	factors					652:658	the design factors	641:658	the design factors	641:658	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	1	9	theme	captopril	165:173	arg1	film					196:199	a patient-friendly captopril fast-dissolving oral film	146:199	a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time	146:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	9	10	theme	acceptable	1037:1046	arg1	content					1059:1065	an acceptable uniformity content	1034:1065	an acceptable uniformity content	1034:1065	All formulations showed an acceptable uniformity content and dissolution rate.
29728997	4	11	theme	surface	497:503	arg1	methodology					505:515	a response surface methodology	486:515	a response surface methodology	486:515	In order to find an optimum formulation, a response surface methodology and a central composite design were employed.
29728997	13	12	theme	enhanced	1601:1608	arg1	absorption					1634:1643	enhanced in vivo orotransmucosal absorption	1601:1643	enhanced in vivo orotransmucosal absorption	1601:1643	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	13	13	theme	overall	1526:1532	arg1	results					1534:1540	The overall results	1522:1540	The overall results	1522:1540	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	7	14	theme	pullulan	871:878	arg1	compatibility					829:841	compatibility	829:841	compatibility	829:841	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	7	14	theme	pullulan	871:878	arg1	strength					855:862	tensile strength	847:862	tensile strength	847:862	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	4	15	theme	optimum	465:471	arg1	formulation					473:483	an optimum formulation	462:483	an optimum formulation	462:483	In order to find an optimum formulation, a response surface methodology and a central composite design were employed.
29728997	13	16	theme	in	1610:1611	arg1	absorption					1634:1643	enhanced in vivo orotransmucosal absorption	1601:1643	enhanced in vivo orotransmucosal absorption	1601:1643	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	5	17	theme	glycerine	609:617	arg1	percentages					581:591	The concentration percentages	563:591	The concentration percentages of pullulan and glycerine	563:617	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	5	17	theme	glycerine	609:617	arg1	factors					652:658	the design factors	641:658	the design factors	641:658	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	6	18	theme	at	719:720	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	18	theme	at	719:720	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	18	theme	at	719:720	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	18	theme	at	719:720	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	18	theme	at	719:720	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	12	19	theme	formulation	1361:1371	arg1	comparison					1337:1346	The in vivo comparison	1325:1346	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet	1325:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	11	20	theme	26	1165:1166	arg1	%					1167:1167	%	1167:1167	%	1167:1167	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	6	21	theme	elongation	708:717	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	21	theme	elongation	708:717	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	21	theme	elongation	708:717	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	21	theme	elongation	708:717	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	21	theme	elongation	708:717	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	1	22	theme	fast-dissolving	175:189	arg1	film					196:199	a patient-friendly captopril fast-dissolving oral film	146:199	a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time	146:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	10	23	theme	Complete	1089:1096	arg1	dissolution					1098:1108	Complete dissolution	1089:1108	Complete dissolution for all formulations	1089:1129	Complete dissolution for all formulations occurred within 2 min.
29728997	8	24	theme	glycerine	974:982	arg1	addition					962:969	the addition	958:969	the addition of glycerine	958:982	Also, the rigid nature of CNF reduced the film elongation but the addition of glycerine improved its flexibility.
29728997	11	25	theme	%	1167:1167	arg1	pullulan					1169:1176	26% pullulan	1165:1176	26% pullulan	1165:1176	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	6	26	theme	percent	700:706	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	26	theme	percent	700:706	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	26	theme	percent	700:706	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	26	theme	percent	700:706	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	26	theme	percent	700:706	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	1	27	theme	oral	191:194	arg1	film					196:199	a patient-friendly captopril fast-dissolving oral film	146:199	a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time	146:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	11	28	theme	optimum	1234:1240	arg1	formulations					1242:1253	optimum formulations	1234:1253	optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels	1234:1322	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	5	29	theme	concentration	567:579	arg1	percentages					581:591	The concentration percentages	563:591	The concentration percentages of pullulan and glycerine	563:617	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	5	29	theme	concentration	567:579	arg1	factors					652:658	the design factors	641:658	the design factors	641:658	The concentration percentages of pullulan and glycerine were considered to be the design factors.
29728997	2	30	theme	solvent-casting	325:339	arg1	method					341:346	the solvent-casting method	321:346	the solvent-casting method	321:346	Films were made with pullulan and hydroxypropyl methyl cellulose (HPMC) by using the solvent-casting method.
29728997	7	31	theme	tensile	847:853	arg1	strength					855:862	tensile strength	847:862	tensile strength	847:862	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	11	32	theme	%	1202:1202	arg1	glycerine					1204:1212	5% glycerine	1201:1212	5% glycerine	1201:1212	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	11	33	theme	1	1189:1189	arg1	%					1190:1190	%	1190:1190	%	1190:1190	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	13	34	theme	promising	1577:1585	arg1	FDF					1568:1570	the captopril FDF	1554:1570	the captopril FDF	1554:1570	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	13	34	theme	promising	1577:1585	arg1	candidate					1587:1595	a promising candidate	1575:1595	a promising candidate for enhanced in vivo orotransmucosal absorption	1575:1643	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	11	35	theme	74	1179:1180	arg1	%					1181:1181	%	1181:1181	%	1181:1181	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	6	36	theme	tensile	682:688	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	36	theme	tensile	682:688	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	36	theme	tensile	682:688	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	36	theme	tensile	682:688	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	36	theme	tensile	682:688	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	1	37	theme	satisfactory	206:217	arg1	disintegration					219:232	satisfactory disintegration	206:232	satisfactory disintegration time	206:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	11	38	theme	oral	1285:1288	arg1	films					1290:1294	oral films	1285:1294	oral films	1285:1294	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	3	39	used	used	379:382	arg2	compatibilizer					389:402	a compatibilizer	387:402	a compatibilizer	387:402	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	3	39	used	used	379:382	arg2	CNF					370:372	CNF	370:372	CNF	370:372	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	3	39	used	used	379:382	arg2	nanofiber					359:367	Cellulose nanofiber	349:367	Cellulose nanofiber (CNF)	349:373	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	11	40	theme	%	1181:1181	arg1	HPMC					1183:1186	74% HPMC	1179:1186	74% HPMC	1179:1186	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	1	41	theme	disintegration	219:232	arg1	time					234:237	satisfactory disintegration time	206:237	satisfactory disintegration time	206:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	12	42	with	comparison	1337:1346	arg1	tablet					1414:1419	a conventional captopril sublingual tablet	1378:1419	a conventional captopril sublingual tablet	1378:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	0	43	theme	Oral	77:80	arg1	Films					82:86	Oral Films	77:86	Oral Films	77:86	Formulation, Characterization, and Optimization of Captopril Fast-Dissolving Oral Films.
29728997	11	44	theme	confidence	1306:1315	arg1	levels					1317:1322	95% confidence levels	1302:1322	95% confidence levels	1302:1322	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	13	45	theme	orotransmucosal	1618:1632	arg1	absorption					1634:1643	enhanced in vivo orotransmucosal absorption	1601:1643	enhanced in vivo orotransmucosal absorption	1601:1643	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	11	46	theme	%	1190:1190	arg1	CNF					1192:1194	1% CNF	1189:1194	1% CNF	1189:1194	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	8	47	theme	rigid	906:910	arg1	nature					912:917	the rigid nature	902:917	the rigid nature of CNF	902:924	Also, the rigid nature of CNF reduced the film elongation but the addition of glycerine improved its flexibility.
29728997	0	48	theme	Captopril	51:59	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Formulation, Characterization, and Optimization of Captopril Fast-Dissolving Oral Films.
29728997	0	48	theme	Captopril	51:59	arg1	Optimization					35:46	Optimization	35:46	Optimization	35:46	Formulation, Characterization, and Optimization of Captopril Fast-Dissolving Oral Films.
29728997	0	48	theme	Captopril	51:59	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation, Characterization, and Optimization of Captopril Fast-Dissolving Oral Films.
29728997	7	49	dep	pullulan	871:878	arg1	the					867:869	the	867:869	the	867:869	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	1	50	with	film	196:199	arg1	time					234:237	satisfactory disintegration time	206:237	satisfactory disintegration time	206:237	This work aimed to using optimization study to formulate a patient-friendly captopril fast-dissolving oral film with satisfactory disintegration time.
29728997	12	51	theme	conventional	1380:1391	arg1	tablet					1414:1419	a conventional captopril sublingual tablet	1378:1419	a conventional captopril sublingual tablet	1378:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	52	dep	in	1329:1330	arg1	vivo					1332:1335	vivo	1332:1335	vivo	1332:1335	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	13	53	theme	captopril	1558:1566	arg1	FDF					1568:1570	the captopril FDF	1554:1570	the captopril FDF	1554:1570	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	13	53	theme	captopril	1558:1566	arg1	candidate					1587:1595	a promising candidate	1575:1595	a promising candidate for enhanced in vivo orotransmucosal absorption	1575:1643	The overall results showed that the captopril FDF is a promising candidate for enhanced in vivo orotransmucosal absorption.
29728997	4	54	theme	composite	531:539	arg1	design					541:546	a central composite design	521:546	a central composite design	521:546	In order to find an optimum formulation, a response surface methodology and a central composite design were employed.
29728997	12	55	theme	sublingual	1403:1412	arg1	tablet					1414:1419	a conventional captopril sublingual tablet	1378:1419	a conventional captopril sublingual tablet	1378:1419	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	6	56	theme	Disintegration	661:674	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	56	theme	Disintegration	661:674	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	56	theme	Disintegration	661:674	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	56	theme	Disintegration	661:674	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	56	theme	Disintegration	661:674	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	4	57	theme	central	523:529	arg1	design					541:546	a central composite design	521:546	a central composite design	521:546	In order to find an optimum formulation, a response surface methodology and a central composite design were employed.
29728997	3	58	used	used	422:425	arg2	glycerine					408:416	glycerine	408:416	glycerine	408:416	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	3	58	used	used	422:425	arg2	plasticizer					432:442	a plasticizer	430:442	a plasticizer	430:442	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	12	59	theme	significant	1431:1441	arg1	increase					1443:1450	significant increase	1431:1450	significant increase in AUC (~ 62%) and Cmax (~ 52%)	1431:1482	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	2	60	theme	methyl	288:293	arg1	HPMC					306:309	HPMC	306:309	HPMC	306:309	Films were made with pullulan and hydroxypropyl methyl cellulose (HPMC) by using the solvent-casting method.
29728997	2	60	theme	methyl	288:293	arg1	cellulose					295:303	hydroxypropyl methyl cellulose	274:303	hydroxypropyl methyl cellulose (HPMC)	274:310	Films were made with pullulan and hydroxypropyl methyl cellulose (HPMC) by using the solvent-casting method.
29728997	12	61	theme	optimized	1351:1359	arg1	formulation					1361:1371	optimized formulation	1351:1371	optimized formulation	1351:1371	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	11	62	with	films	1290:1294	arg1	levels					1317:1322	95% confidence levels	1302:1322	95% confidence levels	1302:1322	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	11	63	theme	5	1201:1201	arg1	%					1202:1202	%	1202:1202	%	1202:1202	Films with 26% pullulan, 74% HPMC, 1% CNF, and 5% glycerine were reported to be optimum formulations for captopril fast-dissolving oral films, with 95% confidence levels.
29728997	2	64	theme	hydroxypropyl	274:286	arg1	HPMC					306:309	HPMC	306:309	HPMC	306:309	Films were made with pullulan and hydroxypropyl methyl cellulose (HPMC) by using the solvent-casting method.
29728997	2	64	theme	hydroxypropyl	274:286	arg1	cellulose					295:303	hydroxypropyl methyl cellulose	274:303	hydroxypropyl methyl cellulose (HPMC)	274:310	Films were made with pullulan and hydroxypropyl methyl cellulose (HPMC) by using the solvent-casting method.
29728997	6	65	theme	folding	733:739	arg1	responses					777:785	the responses	773:785	the responses	773:785	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	65	theme	folding	733:739	arg1	time					676:679	Disintegration time	661:679	Disintegration time	661:679	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	65	theme	folding	733:739	arg1	strength					690:697	tensile strength	682:697	tensile strength	682:697	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	65	theme	folding	733:739	arg1	break					722:726	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	65	theme	folding	733:739	arg1	endurance					741:749	folding endurance	733:749	folding endurance	733:749	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	12	66	from	decrease	1496:1503	arg1	AUC					1455:1457	AUC	1455:1457	AUC (~ 62%)	1455:1465	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	66	from	decrease	1496:1503	arg1	Tmax					1508:1511	Tmax	1508:1511	Tmax	1508:1511	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	66	from	decrease	1496:1503	arg1	%					1464:1464	~ 62%	1460:1464	~ 62%	1460:1464	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	66	from	decrease	1496:1503	arg1	Cmax					1471:1474	Cmax	1471:1474	Cmax (~ 52%)	1471:1482	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	66	from	decrease	1496:1503	arg1	%					1481:1481	~ 52%	1477:1481	~ 52%	1477:1481	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	7	67	theme	blend	889:893	arg1	compatibility					829:841	compatibility	829:841	compatibility	829:841	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	7	67	theme	blend	889:893	arg1	strength					855:862	tensile strength	847:862	tensile strength	847:862	The results showed that CNF improved the compatibility and tensile strength of the pullulan and HPMC blend.
29728997	9	68	theme	dissolution	1071:1081	arg1	rate					1083:1086	dissolution rate	1071:1086	dissolution rate	1071:1086	All formulations showed an acceptable uniformity content and dissolution rate.
29728997	3	69	theme	Cellulose	349:357	arg1	CNF					370:372	CNF	370:372	CNF	370:372	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	3	69	theme	Cellulose	349:357	arg1	nanofiber					359:367	Cellulose nanofiber	349:367	Cellulose nanofiber (CNF)	349:373	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	3	69	theme	Cellulose	349:357	arg1	compatibilizer					389:402	a compatibilizer	387:402	a compatibilizer	387:402	Cellulose nanofiber (CNF) was used as a compatibilizer and glycerine was used as a plasticizer.
29728997	9	70	theme	uniformity	1048:1057	arg1	content					1059:1065	an acceptable uniformity content	1034:1065	an acceptable uniformity content	1034:1065	All formulations showed an acceptable uniformity content and dissolution rate.
29728997	8	71	theme	CNF	922:924	arg1	nature					912:917	the rigid nature	902:917	the rigid nature of CNF	902:924	Also, the rigid nature of CNF reduced the film elongation but the addition of glycerine improved its flexibility.
29728997	12	72	from	increase	1443:1450	arg1	Tmax					1508:1511	Tmax	1508:1511	Tmax	1508:1511	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	72	from	increase	1443:1450	arg1	AUC					1455:1457	AUC	1455:1457	AUC (~ 62%)	1455:1465	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	72	from	increase	1443:1450	arg1	%					1464:1464	~ 62%	1460:1464	~ 62%	1460:1464	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	72	from	increase	1443:1450	arg1	Cmax					1471:1474	Cmax	1471:1474	Cmax (~ 52%)	1471:1482	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	72	from	increase	1443:1450	arg1	%					1481:1481	~ 52%	1477:1481	~ 52%	1477:1481	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	4	73	theme	response	488:495	arg1	methodology					505:515	a response surface methodology	486:515	a response surface methodology	486:515	In order to find an optimum formulation, a response surface methodology and a central composite design were employed.
29728997	8	74	theme	film	938:941	arg1	elongation					943:952	the film elongation	934:952	the film elongation	934:952	Also, the rigid nature of CNF reduced the film elongation but the addition of glycerine improved its flexibility.
29728997	6	75	from	break	722:726	arg1	percent					700:706	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	75	from	break	722:726	arg1	elongation					708:717	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	6	75	from	break	722:726	arg1	at					719:720	percent elongation at break	700:726	percent elongation at break	700:726	Disintegration time, tensile strength, percent elongation at break, and folding endurance were considered to be the responses.
29728997	12	76	theme	major	1490:1494	arg1	%					1518:1518	~ 33%	1514:1518	~ 33%	1514:1518	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
29728997	12	76	theme	major	1490:1494	arg1	decrease					1496:1503	a major decrease	1488:1503	a major decrease in Tmax (~ 33%)	1488:1519	The in vivo comparison of optimized formulation with a conventional captopril sublingual tablet exhibited significant increase in AUC (~ 62%) and Cmax (~ 52%) and a major decrease in Tmax (~ 33%).
30804113	5	0	theme	Na-Ca	731:735	arg1	microfibers					765:775	microfibers	765:775	microfibers	765:775	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	0	theme	Na-Ca	731:735	arg1	composite					752:760	Na-Ca chitosan/algal composite	731:760	Na-Ca chitosan/algal composite of microfibers and nanofibers	731:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	0	theme	Na-Ca	731:735	arg1	nanofibers					781:790	nanofibers	781:790	nanofibers	781:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	1	theme	Na-Ca	878:882	arg1	microfibers					912:922	microfibers	912:922	microfibers	912:922	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	1	theme	Na-Ca	878:882	arg1	composite					899:907	Na-Ca chitosan/algal composite	878:907	Na-Ca chitosan/algal composite of microfibers and nanofibers	878:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	1	theme	Na-Ca	878:882	arg1	nanofibers					928:937	nanofibers	928:937	nanofibers	928:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	9	2	located	observed	1249:1256	arg1	parameters					1277:1286	the coagulation parameters	1261:1286	the coagulation parameters	1261:1286	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	2	located	observed	1249:1256	arg2	changes					1236:1242	Significant changes	1224:1242	Significant changes	1224:1242	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	2	located	observed	1249:1256	arg1	numbers					1302:1308	the total numbers	1292:1308	the total numbers of white blood cells and platelets in groups I and II	1292:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	6	3	theme	polymer	859:865	arg1	mixture					867:873	a polymer mixture	857:873	a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers	857:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	9	4	theme	total	1296:1300	arg1	numbers					1302:1308	the total numbers	1292:1308	the total numbers of white blood cells and platelets in groups I and II	1292:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	5	5	theme	chitosan/algal	737:750	arg1	microfibers					765:775	microfibers	765:775	microfibers	765:775	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	5	theme	chitosan/algal	737:750	arg1	composite					752:760	Na-Ca chitosan/algal composite	731:760	Na-Ca chitosan/algal composite of microfibers and nanofibers	731:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	5	theme	chitosan/algal	737:750	arg1	nanofibers					781:790	nanofibers	781:790	nanofibers	781:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	3	6	theme	prototypes	495:504	arg1	effect					481:486	the effect	477:486	the effect of two prototypes of haemostatic dressing in the porcine haemostatic system	477:562	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	1	7	theme	clotting	251:258	arg1	disorders					260:268	clotting disorders	251:268	clotting disorders	251:268	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	0	8	theme	Haemostatic	88:98	arg1	System					100:105	the Porcine Haemostatic System	76:105	the Porcine Haemostatic System	76:105	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	9	9	dep	groups	1348:1353	arg1	II					1361:1362	II	1361:1362	II	1361:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	9	dep	groups	1348:1353	arg1	I					1355:1355	I	1355:1355	I	1355:1355	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	9	dep	groups	1348:1353	arg1	groups					1348:1353	groups I and II	1348:1362	groups I and II	1348:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	5	10	theme	Group	647:651	arg1	I					653:653	Group I	647:653	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.	647:791	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	2	11	dep	coagulation	377:387	arg1	DIC					390:392	DIC	390:392	DIC	390:392	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	1	12	theme	disorders	260:268	arg1	history					240:246	prior history	234:246	prior history of clotting disorders	234:268	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	0	13	theme	Porcine	80:86	arg1	System					100:105	the Porcine Haemostatic System	76:105	the Porcine Haemostatic System	76:105	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	4	14	dep	MATERIALS	565:573	arg1	number					597:602	The total number	587:602	The total number of animals	587:613	MATERIALS AND METHODS The total number of animals used in our experiments was 24.
30804113	6	15	dep	seton	831:835	arg1	gauze					837:841	gauze	837:841	gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers	837:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	3	16	theme	porcine	537:543	arg1	system					557:562	the porcine haemostatic system	533:562	the porcine haemostatic system	533:562	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	5	17	theme	dressing	713:720	arg1	prototype					693:701	the developed prototype	679:701	the developed prototype	679:701	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	1	18	theme	BACKGROUND/AIM	108:121	arg1	Coagulopathy					123:134	BACKGROUND/AIM Coagulopathy	108:134	BACKGROUND/AIM Coagulopathy	108:134	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	9	19	theme	platelets	1335:1343	arg1	numbers					1302:1308	the total numbers	1292:1308	the total numbers of white blood cells and platelets in groups I and II	1292:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	3	20	theme	dressing	521:528	arg1	prototypes					495:504	two prototypes	491:504	two prototypes of haemostatic dressing	491:528	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	11	21	theme	fibrinogen	1524:1533	arg1	concentrations					1535:1548	high fibrinogen concentrations	1519:1548	high fibrinogen concentrations	1519:1548	This, together with high fibrinogen concentrations, which can cause DIC, require further studying.
30804113	10	22	theme	haemostatic	1411:1421	arg1	dressings					1423:1431	The modified haemostatic dressings	1398:1431	The modified haemostatic dressings used in this study	1398:1450	CONCLUSION The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs.
30804113	9	23	theme	white	1313:1317	arg1	cells					1325:1329	white blood cells	1313:1329	white blood cells	1313:1329	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	5	24	theme	nanofibers	781:790	arg1	microfibers					765:775	microfibers	765:775	microfibers	765:775	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	24	theme	nanofibers	781:790	arg1	composite					752:760	Na-Ca chitosan/algal composite	731:760	Na-Ca chitosan/algal composite of microfibers and nanofibers	731:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	24	theme	nanofibers	781:790	arg1	nanofibers					781:790	nanofibers	781:790	nanofibers	781:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	9	25	theme	Significant	1224:1234	arg1	changes					1236:1242	Significant changes	1224:1242	Significant changes	1224:1242	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	8	26	from	days	1193:1196	arg1	7					1176:1176	7	1176:1176	7	1176:1176	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	10	27	dep	CONCLUSION	1387:1396	arg1	produced					1452:1459	produced	1452:1459	produced a strong procoagulant effect in pigs	1452:1496	CONCLUSION The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs.
30804113	9	28	theme	blood	1319:1323	arg1	cells					1325:1329	white blood cells	1313:1329	white blood cells	1313:1329	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	5	29	dep	I	653:653	arg1	treated					666:672	treated	666:672	were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers	661:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	8	30	dep	coagulation	1090:1100	arg1	profiles					1119:1126	profiles:	1119:1127	profiles: before injury	1119:1141	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	30	dep	coagulation	1090:1100	arg1	the					1086:1088	the	1086:1088	the	1086:1088	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	3	31	from	effect	481:486	arg1	system					557:562	the porcine haemostatic system	533:562	the porcine haemostatic system	533:562	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	9	32	theme	cells	1325:1329	arg1	numbers					1302:1308	the total numbers	1292:1308	the total numbers of white blood cells and platelets in groups I and II	1292:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	0	33	theme	Haemostatic	14:24	arg1	Prototypes					35:44	Haemostatic Dressing Prototypes	14:44	Haemostatic Dressing Prototypes	14:44	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	11	34	theme	high	1519:1522	arg1	concentrations					1535:1548	high fibrinogen concentrations	1519:1548	high fibrinogen concentrations	1519:1548	This, together with high fibrinogen concentrations, which can cause DIC, require further studying.
30804113	3	35	theme	haemostatic	545:555	arg1	system					557:562	the porcine haemostatic system	533:562	the porcine haemostatic system	533:562	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	9	36	from	numbers	1302:1308	arg1	II					1361:1362	II	1361:1362	II	1361:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	36	from	numbers	1302:1308	arg1	I					1355:1355	I	1355:1355	I	1355:1355	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	36	from	numbers	1302:1308	arg1	groups					1348:1353	groups I and II	1348:1362	groups I and II	1348:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	2	37	theme	intravascular	363:375	arg1	coagulation					377:387	disseminated intravascular coagulation	350:387	disseminated intravascular coagulation (DIC)	350:393	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	2	38	theme	significant	416:426	arg1	selection					275:283	The selection	271:283	The selection	271:283	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	2	38	theme	significant	416:426	arg1	challenge					428:436	a significant challenge	414:436	a significant challenge	414:436	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	0	39	theme	Prototypes	35:44	arg1	Effect					4:9	The Effect	0:9	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System	0:105	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	6	40	theme	nanofibers	928:937	arg1	microfibers					912:922	microfibers	912:922	microfibers	912:922	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	40	theme	nanofibers	928:937	arg1	composite					899:907	Na-Ca chitosan/algal composite	878:907	Na-Ca chitosan/algal composite of microfibers and nanofibers	878:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	40	theme	nanofibers	928:937	arg1	nanofibers					928:937	nanofibers	928:937	nanofibers	928:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	41	theme	developed	683:691	arg1	prototype					693:701	the developed prototype	679:701	the developed prototype	679:701	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	1	42	dep	stop	186:189	arg1	bleeding					199:206	bleeding	199:206	bleeding	199:206	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	6	43	dep	Group	793:797	arg1	animals					803:809	animals	803:809	Group II: animals	793:809	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	0	44	theme	Dressing	26:33	arg1	Prototypes					35:44	Haemostatic Dressing Prototypes	14:44	Haemostatic Dressing Prototypes	14:44	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	0	45	from	Services	64:71	arg1	System					100:105	the Porcine Haemostatic System	76:105	the Porcine Haemostatic System	76:105	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	3	46	theme	study	455:459	arg1	aim					443:445	The aim	439:445	The aim of this study	439:459	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	5	47	theme	microfibers	765:775	arg1	microfibers					765:775	microfibers	765:775	microfibers	765:775	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	47	theme	microfibers	765:775	arg1	composite					752:760	Na-Ca chitosan/algal composite	731:760	Na-Ca chitosan/algal composite of microfibers and nanofibers	731:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	5	47	theme	microfibers	765:775	arg1	nanofibers					781:790	nanofibers	781:790	nanofibers	781:790	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	48	theme	microfibers	912:922	arg1	microfibers					912:922	microfibers	912:922	microfibers	912:922	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	48	theme	microfibers	912:922	arg1	composite					899:907	Na-Ca chitosan/algal composite	878:907	Na-Ca chitosan/algal composite of microfibers and nanofibers	878:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	48	theme	microfibers	912:922	arg1	nanofibers					928:937	nanofibers	928:937	nanofibers	928:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	10	49	theme	modified	1402:1409	arg1	dressings					1423:1431	The modified haemostatic dressings	1398:1431	The modified haemostatic dressings used in this study	1398:1450	CONCLUSION The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs.
30804113	11	50	dep	require	1572:1578	arg1	studying					1588:1595	studying	1588:1595	require further studying	1572:1595	This, together with high fibrinogen concentrations, which can cause DIC, require further studying.
30804113	9	51	from	groups	1348:1353	arg1	numbers					1302:1308	the total numbers	1292:1308	the total numbers of white blood cells and platelets in groups I and II	1292:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	2	52	theme	disseminated	350:361	arg1	coagulation					377:387	disseminated intravascular coagulation	350:387	disseminated intravascular coagulation (DIC)	350:393	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	10	53	theme	procoagulant	1470:1481	arg1	effect					1483:1488	a strong procoagulant effect	1461:1488	a strong procoagulant effect	1461:1488	CONCLUSION The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs.
30804113	6	54	dep	gauze	837:841	arg1	modified					843:850	modified	843:850	gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers	837:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	9	55	dep	RESULTS	1216:1222	arg1	observed					1249:1256	observed	1249:1256	observed	1249:1256	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	4	56	theme	total	591:595	arg1	number					597:602	The total number	587:602	The total number of animals	587:613	MATERIALS AND METHODS The total number of animals used in our experiments was 24.
30804113	6	57	theme	composite	899:907	arg1	mixture					867:873	a polymer mixture	857:873	a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers	857:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	7	58	theme	non-hemostatic	977:990	arg1	dressing					992:999	non-hemostatic dressing	977:999	non-hemostatic dressing	977:999	Group III: animals were treated with non-hemostatic dressing and this group was the control.
30804113	8	59	from	changes	1075:1081	arg1	fibrinolytic					1106:1117	fibrinolytic	1106:1117	fibrinolytic	1106:1117	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	59	from	changes	1075:1081	arg1	coagulation					1090:1100	coagulation	1090:1100	coagulation	1090:1100	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	9	60	from	platelets	1335:1343	arg1	II					1361:1362	II	1361:1362	II	1361:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	60	from	platelets	1335:1343	arg1	I					1355:1355	I	1355:1355	I	1355:1355	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	60	from	platelets	1335:1343	arg1	groups					1348:1353	groups I and II	1348:1362	groups I and II	1348:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	2	61	theme	procoagulants	288:300	arg1	selection					275:283	The selection	271:283	The selection	271:283	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	2	61	theme	procoagulants	288:300	arg1	challenge					428:436	a significant challenge	414:436	a significant challenge	414:436	The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge.
30804113	8	62	dep	7	1176:1176	arg1	h					1165:1165	24 h	1162:1165	24 h	1162:1165	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	62	dep	7	1176:1176	arg1	i					1144:1144	i	1144:1144	i	1144:1144	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	62	dep	7	1176:1176	arg1	ii					1155:1156	ii	1155:1156	ii	1155:1156	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	62	dep	7	1176:1176	arg1	iii					1168:1170	iii	1168:1170	iii	1168:1170	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	8	62	dep	7	1176:1176	arg1	h					1152:1152	1 h	1150:1152	1 h	1150:1152	Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury.
30804113	3	63	theme	haemostatic	509:519	arg1	dressing					521:528	haemostatic dressing	509:528	haemostatic dressing	509:528	The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system.
30804113	7	64	dep	Group	940:944	arg1	animals					951:957	animals	951:957	Group III: animals	940:957	Group III: animals were treated with non-hemostatic dressing and this group was the control.
30804113	9	65	theme	coagulation	1265:1275	arg1	parameters					1277:1286	the coagulation parameters	1261:1286	the coagulation parameters	1261:1286	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	10	66	theme	strong	1463:1468	arg1	effect					1483:1488	a strong procoagulant effect	1461:1488	a strong procoagulant effect	1461:1488	CONCLUSION The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs.
30804113	5	67	theme	sponge	706:711	arg1	dressing					713:720	sponge dressing	706:720	sponge dressing	706:720	Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	9	68	from	cells	1325:1329	arg1	II					1361:1362	II	1361:1362	II	1361:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	68	from	cells	1325:1329	arg1	I					1355:1355	I	1355:1355	I	1355:1355	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	9	68	from	cells	1325:1329	arg1	groups					1348:1353	groups I and II	1348:1362	groups I and II	1348:1362	RESULTS Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls.
30804113	1	69	theme	prior	234:238	arg1	history					240:246	prior history	234:246	prior history of clotting disorders	234:268	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	0	70	theme	Emergency	54:62	arg1	Services					64:71	the Emergency Services	50:71	the Emergency Services in the Porcine Haemostatic System	50:105	The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System.
30804113	6	71	theme	chitosan/algal	884:897	arg1	microfibers					912:922	microfibers	912:922	microfibers	912:922	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	71	theme	chitosan/algal	884:897	arg1	composite					899:907	Na-Ca chitosan/algal composite	878:907	Na-Ca chitosan/algal composite of microfibers and nanofibers	878:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	6	71	theme	chitosan/algal	884:897	arg1	nanofibers					928:937	nanofibers	928:937	nanofibers	928:937	Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers.
30804113	1	72	used	used	178:181	arg2	dressings					164:172	hemostatic dressings	153:172	hemostatic dressings	153:172	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	1	73	theme	hemostatic	153:162	arg1	dressings					164:172	hemostatic dressings	153:172	hemostatic dressings	153:172	BACKGROUND/AIM Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders.
30804113	4	74	theme	animals	607:613	arg1	number					597:602	The total number	587:602	The total number of animals	587:613	MATERIALS AND METHODS The total number of animals used in our experiments was 24.
29414706	5	0	theme	previous	826:833	arg1	proposals					835:843	previous proposals	826:843	previous proposals for monomer, dimer, tetramer, and octamer	826:885	For IAPs, oligomerization state in solution is one outstanding question, with previous proposals for monomer, dimer, tetramer, and octamer.
29414706	6	1	theme	n-dodecyl-β-D-maltopyranoside	955:983	arg1	solutions					1001:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions	955:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog	955:1056	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	9	2	theme	corresponding	1385:1397	arg1	initio					1402:1407	the corresponding ab initio	1381:1407	the corresponding ab initio consensus model	1381:1423	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	9	2	theme	corresponding	1385:1397	arg1	consistent					1429:1438	consistent	1429:1438	consistent	1429:1438	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	2	3	theme	endoplasmic	370:380	arg1	reticulum					382:390	the endoplasmic reticulum	366:390	the endoplasmic reticulum	366:390	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	9	4	theme	consensus	1409:1417	arg1	model					1419:1423	consensus model	1409:1423	the corresponding ab initio consensus model	1381:1423	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	0	5	theme	Neutron	73:79	arg1	Scattering					81:90	Small Angle Neutron Scattering	61:90	Small Angle Neutron Scattering	61:90	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	10	6	from	protein	1551:1557	arg1	solution					1562:1569	solution	1562:1569	solution	1562:1569	The model is slightly smaller than the crystallographic IAP monomer, suggesting a more compact protein in solution compared with the crystal lattice.
29414706	8	7	theme	D2O	1330:1332	arg1	use					1294:1296	the use	1290:1296	the use of both tail-deuterated DDM and D2O	1290:1332	The signal from the IAP was enhanced by deuteration and, uniquely, scattering from DDM and buffers were matched by the use of both tail-deuterated DDM and D2O.
29414706	2	8	from	peptides	328:335	arg1	reticulum					382:390	the endoplasmic reticulum	366:390	the endoplasmic reticulum	366:390	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	8	from	peptides	328:335	arg1	presenilin					397:406	presenilin	397:406	presenilin	397:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	11	9	theme	oligomeric	1649:1658	arg1	state					1660:1664	the oligomeric state	1645:1664	the oligomeric state of purified IAP in surfactant solution	1645:1703	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	11	10	dep	fully	1738:1742	arg1	contrast-matching					1744:1760	contrast-matching	1744:1760	contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates	1744:1864	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	10	11	theme	IAP	1512:1514	arg1	monomer					1516:1522	the crystallographic IAP monomer	1491:1522	the crystallographic IAP monomer	1491:1522	The model is slightly smaller than the crystallographic IAP monomer, suggesting a more compact protein in solution compared with the crystal lattice.
29414706	11	12	theme	IAP	1678:1680	arg1	state					1660:1664	the oligomeric state	1645:1664	the oligomeric state of purified IAP in surfactant solution	1645:1703	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	6	13	theme	IAP	1045:1047	arg1	ortholog					1049:1056	a microbial IAP ortholog	1033:1056	a microbial IAP ortholog	1033:1056	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	11	14	theme	purified	1669:1676	arg1	IAP					1678:1680	purified IAP	1669:1680	purified IAP	1669:1680	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	3	15	theme	IAPs	592:595	arg1	relationships					575:587	basic structure-function relationships	550:587	basic structure-function relationships of IAPs	550:595	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	5	16	from	state	774:778	arg1	solution					783:790	solution	783:790	solution	783:790	For IAPs, oligomerization state in solution is one outstanding question, with previous proposals for monomer, dimer, tetramer, and octamer.
29414706	5	17	theme	outstanding	799:809	arg1	question					811:818	one outstanding question	795:818	one outstanding question	795:818	For IAPs, oligomerization state in solution is one outstanding question, with previous proposals for monomer, dimer, tetramer, and octamer.
29414706	9	18	theme	ab	1399:1400	arg1	initio					1402:1407	the corresponding ab initio	1381:1407	the corresponding ab initio consensus model	1381:1423	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	9	18	theme	ab	1399:1400	arg1	consistent					1429:1438	consistent	1429:1438	consistent	1429:1438	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	6	19	theme	microbial	1035:1043	arg1	ortholog					1049:1056	a microbial IAP ortholog	1033:1056	a microbial IAP ortholog	1033:1056	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	2	20	from	polypeptides	350:361	arg1	component					423:431	the catalytic component	409:431	the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein	409:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	20	from	polypeptides	350:361	arg1	peptides					328:335	remnant signal peptides	313:335	remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin	313:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	3	21	theme	basic	550:554	arg1	relationships					575:587	basic structure-function relationships	550:587	basic structure-function relationships of IAPs	550:595	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	0	22	theme	Protease	48:55	arg1	Structure					9:17	Solution Structure	0:17	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.	0:91	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	0	23	theme	Aspartyl	39:46	arg1	Protease					48:55	an Intramembrane Aspartyl Protease	22:55	an Intramembrane Aspartyl Protease	22:55	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	2	24	theme	peptide	278:284	arg1	peptidase					286:294	signal peptide peptidase	271:294	signal peptide peptidase	271:294	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	1	25	theme	integral	166:173	arg1	proteases					184:192	integral membrane proteases	166:192	integral membrane proteases	166:192	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	11	26	from	state	1660:1664	arg1	solution					1696:1703	surfactant solution	1685:1703	surfactant solution	1685:1703	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	11	27	theme	other	1800:1804	arg1	proteases					1820:1828	other intramembrane proteases	1800:1828	other intramembrane proteases	1800:1828	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	2	28	theme	signal	321:326	arg1	component					423:431	the catalytic component	409:431	the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein	409:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	28	theme	signal	321:326	arg1	peptides					328:335	remnant signal peptides	313:335	remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin	313:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	3	29	theme	active	604:609	arg1	areas					611:615	active areas	604:615	active areas of research	604:627	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	10	30	theme	crystal	1589:1595	arg1	lattice					1597:1603	the crystal lattice	1585:1603	the crystal lattice	1585:1603	The model is slightly smaller than the crystallographic IAP monomer, suggesting a more compact protein in solution compared with the crystal lattice.
29414706	1	31	theme	membrane	175:182	arg1	proteases					184:192	integral membrane proteases	166:192	integral membrane proteases	166:192	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	0	32	theme	Solution	0:7	arg1	Structure					9:17	Solution Structure	0:17	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.	0:91	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	11	33	theme	intramembrane	1806:1818	arg1	proteases					1820:1828	other intramembrane proteases	1800:1828	other intramembrane proteases	1800:1828	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	1	34	theme	Intramembrane	93:105	arg1	IAPs					127:130	IAPs	127:130	IAPs	127:130	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	1	34	theme	Intramembrane	93:105	arg1	proteases					116:124	Intramembrane aspartyl proteases	93:124	Intramembrane aspartyl proteases (IAPs)	93:131	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	1	35	theme	proteases	184:192	arg1	families					154:161	one of four families	142:161	one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer	142:255	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	2	36	theme	complex	452:458	arg1	component					423:431	the catalytic component	409:431	the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein	409:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	36	theme	complex	452:458	arg1	peptides					328:335	remnant signal peptides	313:335	remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin	313:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	5	37	theme	oligomerization	758:772	arg1	state					774:778	oligomerization state	758:778	oligomerization state in solution	758:790	For IAPs, oligomerization state in solution is one outstanding question, with previous proposals for monomer, dimer, tetramer, and octamer.
29414706	1	38	theme	aspartyl	107:114	arg1	IAPs					127:130	IAPs	127:130	IAPs	127:130	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	1	38	theme	aspartyl	107:114	arg1	proteases					116:124	Intramembrane aspartyl proteases	93:124	Intramembrane aspartyl proteases (IAPs)	93:131	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	2	39	theme	γ-secretase	440:450	arg1	complex					452:458	the γ-secretase complex	436:458	the γ-secretase complex	436:458	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	7	40	theme	solvent	1115:1121	arg1	composition					1123:1133	the solvent composition	1111:1133	the solvent composition to mask all but the enzyme of interest	1111:1172	A unique feature of SANS is the ability to modulate the solvent composition to mask all but the enzyme of interest.
29414706	6	41	theme	neutron	913:919	arg1	SANS					933:936	SANS	933:936	SANS	933:936	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	6	41	theme	neutron	913:919	arg1	scattering					921:930	small angle neutron scattering	901:930	small angle neutron scattering (SANS)	901:937	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	2	42	theme	precursor	493:501	arg1	protein					503:509	Notch and amyloid precursor protein	475:509	protein	503:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	9	43	theme	gyration	1349:1356	arg1	initio					1402:1407	the corresponding ab initio	1381:1407	the corresponding ab initio consensus model	1381:1423	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	9	43	theme	gyration	1349:1356	arg1	radius					1339:1344	The radius	1335:1344	The radius of gyration calculated for IAP	1335:1375	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	9	43	theme	gyration	1349:1356	arg1	consistent					1429:1438	consistent	1429:1438	consistent	1429:1438	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	6	44	theme	angle	907:911	arg1	SANS					933:936	SANS	933:936	SANS	933:936	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	6	44	theme	angle	907:911	arg1	scattering					921:930	small angle neutron scattering	901:930	small angle neutron scattering (SANS)	901:937	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	6	45	used	used	896:899	arg2	we					893:894	we	893:894	we	893:894	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	4	46	theme	hydrophobic	725:735	arg1	character					737:745	their inherently hydrophobic character	708:745	their inherently hydrophobic character	708:745	Characterization of membrane-bound proteins is notoriously challenging due to their inherently hydrophobic character.
29414706	0	47	theme	Intramembrane	25:37	arg1	Protease					48:55	an Intramembrane Aspartyl Protease	22:55	an Intramembrane Aspartyl Protease	22:55	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	11	48	theme	membrane-bound	1840:1853	arg1	substrates					1855:1864	their membrane-bound substrates	1834:1864	their membrane-bound substrates	1834:1864	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	2	49	theme	amyloid	485:491	arg1	protein					503:509	Notch and amyloid precursor protein	475:509	protein	503:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	6	50	theme	small	901:905	arg1	SANS					933:936	SANS	933:936	SANS	933:936	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	6	50	theme	small	901:905	arg1	scattering					921:930	small angle neutron scattering	901:930	small angle neutron scattering (SANS)	901:937	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	5	51	with	question	811:818	arg1	proposals					835:843	previous proposals	826:843	previous proposals for monomer, dimer, tetramer, and octamer	826:885	For IAPs, oligomerization state in solution is one outstanding question, with previous proposals for monomer, dimer, tetramer, and octamer.
29414706	2	52	theme	catalytic	413:421	arg1	component					423:431	the catalytic component	409:431	the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein	409:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	52	theme	catalytic	413:421	arg1	peptides					328:335	remnant signal peptides	313:335	remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin	313:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	6	53	theme	detergent	991:999	arg1	solutions					1001:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions	955:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog	955:1056	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	10	54	theme	compact	1543:1549	arg1	protein					1551:1557	a more compact protein	1536:1557	a more compact protein in solution compared with the crystal lattice	1536:1603	The model is slightly smaller than the crystallographic IAP monomer, suggesting a more compact protein in solution compared with the crystal lattice.
29414706	9	55	with	consistent	1429:1438	arg1	monomer					1447:1453	a monomer	1445:1453	a monomer	1445:1453	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	2	56	theme	Notch	475:479	arg1	protein					503:509	Notch and amyloid precursor protein	475:509	protein	503:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	10	57	theme	crystallographic	1495:1510	arg1	monomer					1516:1522	the crystallographic IAP monomer	1491:1522	the crystallographic IAP monomer	1491:1522	The model is slightly smaller than the crystallographic IAP monomer, suggesting a more compact protein in solution compared with the crystal lattice.
29414706	3	58	theme	research	620:627	arg1	areas					611:615	active areas	604:615	active areas of research	604:627	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	8	59	from	DDM	1258:1260	arg1	scattering					1242:1251	scattering	1242:1251	scattering	1242:1251	The signal from the IAP was enhanced by deuteration and, uniquely, scattering from DDM and buffers were matched by the use of both tail-deuterated DDM and D2O.
29414706	3	60	dep	biomedical	532:541	arg1	reach					543:547	reach	543:547	reach	543:547	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	6	61	theme	DDM	986:988	arg1	solutions					1001:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions	955:1009	n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog	955:1056	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	7	62	theme	SANS	1079:1082	arg1	ability					1091:1097	the ability to modulate the solvent composition to mask all but the enzyme of interest	1087:1172	the ability to modulate the solvent composition to mask all but the enzyme of interest	1087:1172	A unique feature of SANS is the ability to modulate the solvent composition to mask all but the enzyme of interest.
29414706	7	62	theme	SANS	1079:1082	arg1	feature					1068:1074	A unique feature	1059:1074	A unique feature of SANS	1059:1082	A unique feature of SANS is the ability to modulate the solvent composition to mask all but the enzyme of interest.
29414706	8	63	from	IAP	1195:1197	arg1	signal					1179:1184	The signal	1175:1184	The signal from the IAP	1175:1197	The signal from the IAP was enhanced by deuteration and, uniquely, scattering from DDM and buffers were matched by the use of both tail-deuterated DDM and D2O.
29414706	2	64	theme	signal	271:276	arg1	peptidase					286:294	signal peptide peptidase	271:294	signal peptide peptidase	271:294	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	7	65	theme	unique	1061:1066	arg1	ability					1091:1097	the ability to modulate the solvent composition to mask all but the enzyme of interest	1087:1172	the ability to modulate the solvent composition to mask all but the enzyme of interest	1087:1172	A unique feature of SANS is the ability to modulate the solvent composition to mask all but the enzyme of interest.
29414706	7	65	theme	unique	1061:1066	arg1	feature					1068:1074	A unique feature	1059:1074	A unique feature of SANS	1059:1082	A unique feature of SANS is the ability to modulate the solvent composition to mask all but the enzyme of interest.
29414706	2	66	theme	nascent	342:348	arg1	polypeptides					350:361	nascent polypeptides	342:361	nascent polypeptides	342:361	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	67	theme	remnant	313:319	arg1	component					423:431	the catalytic component	409:431	the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein	409:509	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	2	67	theme	remnant	313:319	arg1	peptides					328:335	remnant signal peptides	313:335	remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin	313:406	IAPs include signal peptide peptidase, which processes remnant signal peptides from nascent polypeptides in the endoplasmic reticulum, and presenilin, the catalytic component of the γ-secretase complex that processes Notch and amyloid precursor protein.
29414706	4	68	theme	membrane-bound	650:663	arg1	proteins					665:672	membrane-bound proteins	650:672	membrane-bound proteins	650:672	Characterization of membrane-bound proteins is notoriously challenging due to their inherently hydrophobic character.
29414706	3	69	theme	structure-function	556:573	arg1	relationships					575:587	basic structure-function relationships	550:587	basic structure-function relationships of IAPs	550:595	Despite their broad biomedical reach, basic structure-function relationships of IAPs remain active areas of research.
29414706	6	70	dep	solutions	1001:1009	arg1	absent					1026:1031	absent	1026:1031	absent	1026:1031	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	6	70	dep	solutions	1001:1009	arg1	containing					1011:1020	containing	1011:1020	containing	1011:1020	Here we used small angle neutron scattering (SANS) to characterize n-dodecyl-β-D-maltopyranoside (DDM) detergent solutions containing and absent a microbial IAP ortholog.
29414706	1	71	theme	hydrophobic	231:241	arg1	bilayer					249:255	the hydrophobic lipid bilayer	227:255	the hydrophobic lipid bilayer	227:255	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	0	72	theme	Angle	67:71	arg1	Scattering					81:90	Small Angle Neutron Scattering	61:90	Small Angle Neutron Scattering	61:90	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	11	73	theme	direct	1625:1630	arg1	insight					1632:1638	direct insight	1625:1638	direct insight into the oligomeric state of purified IAP in surfactant solution	1625:1703	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	9	74	dep	initio	1402:1407	arg1	model					1419:1423	consensus model	1409:1423	the corresponding ab initio consensus model	1381:1423	The radius of gyration calculated for IAP and the corresponding ab initio consensus model are consistent with a monomer.
29414706	1	75	theme	lipid	243:247	arg1	bilayer					249:255	the hydrophobic lipid bilayer	227:255	the hydrophobic lipid bilayer	227:255	Intramembrane aspartyl proteases (IAPs) comprise one of four families of integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer.
29414706	0	76	theme	Small	61:65	arg1	Scattering					81:90	Small Angle Neutron Scattering	61:90	Small Angle Neutron Scattering	61:90	Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.
29414706	11	77	dep	characterize	1787:1798	arg1	SANS					1779:1782	SANS	1779:1782	SANS	1779:1782	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	11	78	theme	fully	1738:1742	arg1	utility					1727:1733	the utility	1723:1733	the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates	1723:1864	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	8	79	theme	DDM	1322:1324	arg1	use					1294:1296	the use	1290:1296	the use of both tail-deuterated DDM and D2O	1290:1332	The signal from the IAP was enhanced by deuteration and, uniquely, scattering from DDM and buffers were matched by the use of both tail-deuterated DDM and D2O.
29414706	11	80	theme	surfactant	1685:1694	arg1	solution					1696:1703	surfactant solution	1685:1703	surfactant solution	1685:1703	Our study provides direct insight into the oligomeric state of purified IAP in surfactant solution, and demonstrates the utility of fully contrast-matching the detergent in SANS to characterize other intramembrane proteases and their membrane-bound substrates.
29414706	4	81	theme	proteins	665:672	arg1	Characterization					630:645	Characterization	630:645	Characterization of membrane-bound proteins	630:672	Characterization of membrane-bound proteins is notoriously challenging due to their inherently hydrophobic character.
30742921	7	0	theme	reinforcing	1097:1107	arg1	mechanism					1109:1117	the reinforcing mechanism	1093:1117	the reinforcing mechanism	1093:1117	The cellulose containing in laver kept its semi-crystalline structure after processing, which was used to explain the reinforcing mechanism.
30742921	4	1	theme	fiber	667:671	arg1	composites					680:689	a starch-protein-laver fiber hybrid composites	644:689	a starch-protein-laver fiber hybrid composites	644:689	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	5	2	theme	mechanical	734:743	arg1	properties					745:754	the mechanical properties	730:754	the mechanical properties of the starch-based film about 25%	730:789	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	9	3	theme	food	1262:1265	arg1	sources					1267:1273	food sources	1262:1273	food sources	1262:1273	Since all the components used in this work are from food sources, the prepared films are safe for food packaging and application as edible films.
30742921	5	4	dep	%	789:789	arg1	25					787:788	25	787:788	25	787:788	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	4	5	theme	starch-protein-laver	646:665	arg1	composites					680:689	a starch-protein-laver fiber hybrid composites	644:689	a starch-protein-laver fiber hybrid composites	644:689	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	5	6	dep	film	776:779	arg1	%					789:789	%	789:789	%	789:789	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	5	7	theme	hybrids	699:705	arg1	system					707:712	the hybrids system	695:712	the hybrids system	695:712	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	5	8	theme	film	776:779	arg1	properties					745:754	the mechanical properties	730:754	the mechanical properties of the starch-based film about 25%	730:789	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	3	9	theme	reinforcing	340:350	arg1	agent					352:356	reinforcing agent	340:356	reinforcing agent	340:356	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	9	10	theme	prepared	1280:1287	arg1	safe					1299:1302	safe	1299:1302	safe	1299:1302	Since all the components used in this work are from food sources, the prepared films are safe for food packaging and application as edible films.
30742921	9	10	theme	prepared	1280:1287	arg1	films					1289:1293	the prepared films	1276:1293	the prepared films	1276:1293	Since all the components used in this work are from food sources, the prepared films are safe for food packaging and application as edible films.
30742921	0	11	theme	starch	43:48	arg1	film					50:53	edible starch film	36:53	edible starch film	36:53	Preparation and characterization of edible starch film reinforced by laver.
30742921	1	12	theme	Edible	76:81	arg1	film					90:93	Edible starch film	76:93	Edible starch film reinforced by laver	76:113	Edible starch film reinforced by laver was developed and characterized.
30742921	3	13	theme	mechanical	373:382	arg1	properties					384:393	the mechanical properties	369:393	the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength	369:472	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	6	14	theme	good	927:930	arg1	compatibility					932:944	good compatibility	927:944	good compatibility between starch matrix and laver	927:976	Scanning electron microscopic observation indicated good compatibility between starch matrix and laver.
30742921	1	15	theme	starch	83:88	arg1	film					90:93	Edible starch film	76:93	Edible starch film reinforced by laver	76:113	Edible starch film reinforced by laver was developed and characterized.
30742921	7	16	theme	semi-crystalline	1022:1037	arg1	structure					1039:1047	its semi-crystalline structure	1018:1047	its semi-crystalline structure	1018:1047	The cellulose containing in laver kept its semi-crystalline structure after processing, which was used to explain the reinforcing mechanism.
30742921	5	17	theme	moisture	822:829	arg1	sensitivity					831:841	moisture sensitivity	822:841	moisture sensitivity	822:841	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	6	18	theme	starch	954:959	arg1	matrix					961:966	starch matrix	954:966	starch matrix	954:966	Scanning electron microscopic observation indicated good compatibility between starch matrix and laver.
30742921	8	19	theme	chemical	1125:1132	arg1	bounds					1134:1139	Some chemical bounds	1120:1139	Some chemical bounds between starch and protein containing in laver	1120:1186	Some chemical bounds between starch and protein containing in laver was detected by FTIR.
30742921	3	20	theme	tensile	457:463	arg1	strength					465:472	tensile strength	457:472	tensile strength	457:472	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	3	21	theme	starch	402:407	arg1	matrix					409:414	the starch matrix	398:414	the starch matrix	398:414	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	4	22	theme	shear	543:547	arg1	stress					549:554	shear stress	543:554	shear stress	543:554	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	3	23	theme	cold	278:281	arg1	suspension					290:299	cold starch suspension	278:299	cold starch suspension	278:299	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	0	24	theme	edible	36:41	arg1	film					50:53	edible starch film	36:53	edible starch film	36:53	Preparation and characterization of edible starch film reinforced by laver.
30742921	4	25	theme	starch	509:514	arg1	suspension					516:525	hot starch suspension	505:525	hot starch suspension	505:525	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	3	26	theme	starch	283:288	arg1	suspension					290:299	cold starch suspension	278:299	cold starch suspension	278:299	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	2	27	theme	processing	173:182	arg1	technologies					184:195	processing technologies	173:195	processing technologies	173:195	The relationship between processing technologies, microstructures and performances was established.
30742921	5	28	dep	sensitivity	831:841	arg1	the					818:820	the	818:820	the	818:820	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	7	29	used	used	1077:1080	arg2	processing					1055:1064	processing	1055:1064	processing	1055:1064	The cellulose containing in laver kept its semi-crystalline structure after processing, which was used to explain the reinforcing mechanism.
30742921	4	30	theme	hot	505:507	arg1	suspension					516:525	hot starch suspension	505:525	hot starch suspension	505:525	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	5	31	theme	starch-based	763:774	arg1	film					776:779	the starch-based film	759:779	the starch-based film about 25%	759:789	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
30742921	0	32	theme	film	50:53	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of edible starch film reinforced by laver.
30742921	0	32	theme	film	50:53	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of edible starch film reinforced by laver.
30742921	6	33	theme	microscopic	893:903	arg1	observation					905:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation indicated good compatibility between starch matrix and laver.
30742921	6	34	theme	Scanning	875:882	arg1	observation					905:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation indicated good compatibility between starch matrix and laver.
30742921	4	35	theme	hybrid	673:678	arg1	composites					680:689	a starch-protein-laver fiber hybrid composites	644:689	a starch-protein-laver fiber hybrid composites	644:689	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	6	36	theme	electron	884:891	arg1	observation					905:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation	875:915	Scanning electron microscopic observation indicated good compatibility between starch matrix and laver.
30742921	4	37	with	mix	610:612	arg1	starch					619:624	starch	619:624	starch	619:624	When the laver was added into hot starch suspension and mixed under shear stress, the protein containing in laver will release out and mix with starch, which results in a starch-protein-laver fiber hybrid composites.
30742921	9	38	theme	food	1308:1311	arg1	packaging					1313:1321	food packaging	1308:1321	food packaging	1308:1321	Since all the components used in this work are from food sources, the prepared films are safe for food packaging and application as edible films.
30742921	9	39	theme	edible	1342:1347	arg1	films					1349:1353	edible films	1342:1353	edible films	1342:1353	Since all the components used in this work are from food sources, the prepared films are safe for food packaging and application as edible films.
30742921	3	40	theme	laver	306:310	arg1	fiber					319:323	fiber	319:323	fiber	319:323	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	3	40	theme	laver	306:310	arg1	flack					312:316	the laver flack	302:316	the laver flack (fiber)	302:324	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	3	41	theme	matrix	409:414	arg1	properties					384:393	the mechanical properties	369:393	the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength	369:472	When the laver was added into cold starch suspension, the laver flack (fiber) simply act as reinforcing agent to improve the mechanical properties of the starch matrix, which results in increasing modulus and tensile strength.
30742921	5	42	theme	gas	847:849	arg1	permeability					851:862	gas permeability	847:862	gas permeability	847:862	In the hybrids system, fiber improves the mechanical properties of the starch-based film about 25%, while the protein reduces the moisture sensitivity and gas permeability about 45%.
29951672	4	0	theme	scanning	1062:1069	arg1	microscopy					1080:1089	scanning electron microscopy	1062:1089	scanning electron microscopy (SEM)	1062:1095	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	0	theme	scanning	1062:1069	arg1	SEM					1092:1094	SEM	1092:1094	SEM	1092:1094	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	3	1	theme	formed	694:699	arg1	colloids					701:708	The formed colloids	690:708	The formed colloids in the bulk	690:720	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	3	2	from	parameters	889:898	arg1	regimes					939:945	different concentration regimes	915:945	different concentration regimes	915:945	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	1	3	with	counterparts	477:488	arg1	NaCMC					526:530	NaCMC	526:530	NaCMC	526:530	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	3	with	counterparts	477:488	arg1	carboxymethylcellulose					502:523	sodium carboxymethylcellulose	495:523	sodium carboxymethylcellulose (NaCMC)	495:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	8	4	theme	physical	1843:1850	arg1	insights					1852:1859	physical insights	1843:1859	physical insights into the complexation phenomenon	1843:1892	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	2	5	theme	air-solution	626:637	arg1	interface					639:647	the air-solution interface	622:647	the air-solution interface	622:647	The behaviour of colloidal systems comprising SAILs and NaCMC at the air-solution interface has been investigated using tensiometry.
29951672	0	6	theme	Colloidal	0:8	arg1	systems					10:16	Colloidal systems	0:16	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.	0:160	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	7	theme	active	196:201	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	chloride					256:263	1-methyl-3-dodecylimidazolium chloride	226:263	1-methyl-3-dodecylimidazolium chloride	226:263	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	ester					383:387	ester	383:387	ester	383:387	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	counterparts					477:488	functionalized counterparts	462:488	functionalized counterparts with sodium carboxymethylcellulose (NaCMC)	462:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	[C12mim					266:272	[C12mim	266:272	[C12mim][Cl]	266:277	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	amide					288:292	its amide	284:292	its amide	284:292	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	7	theme	active	196:201	arg1	SAILs					218:222	SAILs	218:222	SAILs	218:222	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	3	8	from	hydrophobicity/hydrophilicity	835:863	arg1	regimes					939:945	different concentration regimes	915:945	different concentration regimes	915:945	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	8	9	theme	complexation	1870:1881	arg1	phenomenon					1883:1892	the complexation phenomenon	1866:1892	the complexation phenomenon	1866:1892	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	1	10	theme	liquids	209:215	arg1	complexation					166:177	The complexation	162:177	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC),	162:532	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	6	11	theme	formed	1322:1327	arg1	complexes					1329:1337	The formed complexes	1318:1337	The formed complexes of SAILs and NaCMC	1318:1356	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	1	12	dep	[C12mim	266:272	arg1	[Cl					274:276	[Cl	274:276	[Cl	274:276	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	3	13	from	colloids	701:708	arg1	bulk					717:720	the bulk	713:720	the bulk	713:720	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	0	14	theme	nano-composites	145:159	arg1	investigations					102:115	physicochemical investigations	86:115	physicochemical investigations	86:115	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	0	14	theme	nano-composites	145:159	arg1	preparation					121:131	preparation	121:131	preparation	121:131	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	6	15	theme	magnetic	1408:1415	arg1	nano-composites					1417:1431	magnetic nano-composites	1408:1431	magnetic nano-composites	1408:1431	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	7	16	theme	transmission	1602:1613	arg1	microscopy					1624:1633	transmission electron microscopy	1602:1633	transmission electron microscopy (TEM)	1602:1639	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	7	16	theme	transmission	1602:1613	arg1	TEM					1636:1638	TEM	1636:1638	TEM	1636:1638	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	2	17	theme	colloidal	574:582	arg1	systems					584:590	colloidal systems	574:590	colloidal systems comprising SAILs and NaCMC at the air-solution interface	574:647	The behaviour of colloidal systems comprising SAILs and NaCMC at the air-solution interface has been investigated using tensiometry.
29951672	3	18	theme	surface	766:772	arg1	charge					774:779	surface charge	766:779	surface charge	766:779	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	8	19	theme	new	1733:1735	arg1	route					1747:1751	a new colloidal route	1731:1751	a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites	1731:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	3	20	theme	other	869:873	arg1	parameters					889:898	other thermodynamic parameters	869:898	other thermodynamic parameters of interest in different concentration regimes of the SAILs	869:958	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	5	21	dep	[Cl	1202:1204	arg1	[C12Emim					1193:1200	[C12Emim	1193:1200	[C12Emim	1193:1200	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	0	22	theme	sodium	54:59	arg1	cellulose					75:83	sodium carboxymethyl cellulose	54:83	sodium carboxymethyl cellulose	54:83	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	23	theme	dodecylamino	301:312	arg1	[Cl					373:375	2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl	298:375	[Cl	373:375	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	23	theme	dodecylamino	301:312	arg1	3-					295:296	3-	295:296	3-	295:296	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	3	24	theme	interest	903:910	arg1	hydrophobicity/hydrophilicity					835:863	their relative hydrophobicity/hydrophilicity	820:863	their relative hydrophobicity/hydrophilicity	820:863	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	3	24	theme	interest	903:910	arg1	parameters					889:898	other thermodynamic parameters	869:898	other thermodynamic parameters of interest in different concentration regimes of the SAILs	869:958	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	7	25	theme	X-ray	1577:1581	arg1	XRD					1596:1598	XRD	1596:1598	XRD	1596:1598	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	7	25	theme	X-ray	1577:1581	arg1	diffraction					1583:1593	X-ray diffraction	1577:1593	X-ray diffraction (XRD)	1577:1599	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	0	26	theme	cellulose	75:83	arg1	systems					10:16	Colloidal systems	0:16	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.	0:160	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	27	theme	-2-oxoethyl	314:324	arg1	[Cl					373:375	2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl	298:375	[Cl	373:375	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	27	theme	-2-oxoethyl	314:324	arg1	3-					295:296	3-	295:296	3-	295:296	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	0	28	theme	physicochemical	86:100	arg1	investigations					102:115	physicochemical investigations	86:115	physicochemical investigations	86:115	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	29	theme	-1-methyl-1H-imidazol-3-ium	326:352	arg1	[Cl					373:375	2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl	298:375	[Cl	373:375	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	29	theme	-1-methyl-1H-imidazol-3-ium	326:352	arg1	3-					295:296	3-	295:296	3-	295:296	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	8	30	theme	assisted	1797:1804	arg1	nano-composites					1806:1820	SAILs and biopolymer assisted nano-composites	1776:1820	SAILs and biopolymer assisted nano-composites	1776:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	3	31	theme	different	915:923	arg1	regimes					939:945	different concentration regimes	915:945	different concentration regimes	915:945	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	6	32	theme	nano-composites	1417:1431	arg1	preparation					1393:1403	the one pot preparation	1381:1403	the one pot preparation of magnetic nano-composites	1381:1431	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	7	33	theme	vibrating	1645:1653	arg1	VSM					1676:1678	VSM	1676:1678	VSM	1676:1678	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	7	33	theme	vibrating	1645:1653	arg1	magnetometry					1662:1673	vibrating sample magnetometry	1645:1673	vibrating sample magnetometry (VSM)	1645:1679	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	6	34	theme	pot	1389:1391	arg1	preparation					1393:1403	the one pot preparation	1381:1403	the one pot preparation of magnetic nano-composites	1381:1431	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	0	35	theme	active	29:34	arg1	liquids					42:48	surface active ionic liquids	21:48	surface active ionic liquids	21:48	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	4	36	theme	light	1031:1035	arg1	scattering					1037:1046	dynamic light scattering	1023:1046	dynamic light scattering	1023:1046	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	0	37	theme	liquids	42:48	arg1	systems					10:16	Colloidal systems	0:16	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.	0:160	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	38	theme	1-methyl-3-dodecylimidazolium	226:254	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	38	theme	1-methyl-3-dodecylimidazolium	226:254	arg1	chloride					256:263	1-methyl-3-dodecylimidazolium chloride	226:263	1-methyl-3-dodecylimidazolium chloride	226:263	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	4	39	theme	fluorescence	1101:1112	arg1	measurements					1114:1125	fluorescence measurements	1101:1125	fluorescence measurements	1101:1125	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	40	theme	electron	1071:1078	arg1	microscopy					1080:1089	scanning electron microscopy	1062:1089	scanning electron microscopy (SEM)	1062:1095	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	40	theme	electron	1071:1078	arg1	SEM					1092:1094	SEM	1092:1094	SEM	1092:1094	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	1	41	dep	[Cl	373:375	arg1	[C12Amim					364:371	2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl	298:375	[C12Amim	364:371	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	6	42	with	NaCMC	1465:1469	arg1	nano-particles					1499:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	8	43	dep	offer	1725:1729	arg1	providing					1833:1841	providing	1833:1841	providing physical insights into the complexation phenomenon	1833:1892	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	6	44	theme	doping	1436:1441	arg1	colloids					1443:1450	doping colloids	1436:1450	doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles	1436:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	2	45	theme	systems	584:590	arg1	behaviour					561:569	The behaviour	557:569	The behaviour of colloidal systems comprising SAILs and NaCMC at the air-solution interface	557:647	The behaviour of colloidal systems comprising SAILs and NaCMC at the air-solution interface has been investigated using tensiometry.
29951672	1	46	theme	surface	188:194	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	chloride					256:263	1-methyl-3-dodecylimidazolium chloride	226:263	1-methyl-3-dodecylimidazolium chloride	226:263	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	ester					383:387	ester	383:387	ester	383:387	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	counterparts					477:488	functionalized counterparts	462:488	functionalized counterparts with sodium carboxymethylcellulose (NaCMC)	462:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	[C12mim					266:272	[C12mim	266:272	[C12mim][Cl]	266:277	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	amide					288:292	its amide	284:292	its amide	284:292	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	46	theme	surface	188:194	arg1	SAILs					218:222	SAILs	218:222	SAILs	218:222	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	6	47	with	SAILs	1455:1459	arg1	nano-particles					1499:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	1	48	theme	ionic	203:207	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	chloride					256:263	1-methyl-3-dodecylimidazolium chloride	226:263	1-methyl-3-dodecylimidazolium chloride	226:263	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	ester					383:387	ester	383:387	ester	383:387	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	counterparts					477:488	functionalized counterparts	462:488	functionalized counterparts with sodium carboxymethylcellulose (NaCMC)	462:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	[C12mim					266:272	[C12mim	266:272	[C12mim][Cl]	266:277	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	amide					288:292	its amide	284:292	its amide	284:292	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	48	theme	ionic	203:207	arg1	SAILs					218:222	SAILs	218:222	SAILs	218:222	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	5	49	theme	prone	1157:1161	arg1	SAILs					1163:1167	H-bonding prone SAILs	1147:1167	H-bonding prone SAILs	1147:1167	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	0	50	theme	magnetic	136:143	arg1	nano-composites					145:159	magnetic nano-composites	136:159	magnetic nano-composites	136:159	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	51	theme	functionalized	462:475	arg1	counterparts					477:488	functionalized counterparts	462:488	functionalized counterparts with sodium carboxymethylcellulose (NaCMC)	462:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	51	theme	functionalized	462:475	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	6	52	theme	ZnFe3O4	1490:1496	arg1	nano-particles					1499:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	7	53	theme	electron	1615:1622	arg1	microscopy					1624:1633	transmission electron microscopy	1602:1633	transmission electron microscopy (TEM)	1602:1639	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	7	53	theme	electron	1615:1622	arg1	TEM					1636:1638	TEM	1636:1638	TEM	1636:1638	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	6	54	theme	ferrite	1481:1487	arg1	nano-particles					1499:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	5	55	theme	contrasting	1246:1256	arg1	manner					1258:1263	a contrasting manner	1244:1263	a contrasting manner	1244:1263	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	3	56	theme	thermodynamic	875:887	arg1	parameters					889:898	other thermodynamic parameters	869:898	other thermodynamic parameters of interest in different concentration regimes of the SAILs	869:958	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	0	57	theme	carboxymethyl	61:73	arg1	cellulose					75:83	sodium carboxymethyl cellulose	54:83	sodium carboxymethyl cellulose	54:83	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	1	58	theme	3-methyl-1-dodecyloxycarbonylmethylimidazolium	390:435	arg1	ester					383:387	ester	383:387	ester	383:387	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	58	theme	3-methyl-1-dodecyloxycarbonylmethylimidazolium	390:435	arg1	chloride					437:444	3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride	390:444	3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride	390:444	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	8	59	theme	colloidal	1737:1745	arg1	route					1747:1751	a new colloidal route	1731:1751	a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites	1731:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	1	60	dep	amide	288:292	arg1	[Cl					456:458	[Cl	456:458	[Cl	456:458	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	5	61	theme	H-bonding	1147:1155	arg1	SAILs					1163:1167	H-bonding prone SAILs	1147:1167	H-bonding prone SAILs	1147:1167	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	5	62	dep	[Cl	1184:1186	arg1	[C12Amim					1175:1182	[C12Amim	1175:1182	[C12Amim	1175:1182	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	5	62	dep	[Cl	1184:1186	arg1	i.e.					1170:1173	i.e.	1170:1173	i.e.	1170:1173	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	3	63	theme	relative	826:833	arg1	hydrophobicity/hydrophilicity					835:863	their relative hydrophobicity/hydrophilicity	820:863	their relative hydrophobicity/hydrophilicity	820:863	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	6	64	theme	NaCMC	1352:1356	arg1	complexes					1329:1337	The formed complexes	1318:1337	The formed complexes of SAILs and NaCMC	1318:1356	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	7	65	theme	magnetic	1528:1535	arg1	nano-composites					1537:1551	The prepared magnetic nano-composites	1515:1551	The prepared magnetic nano-composites	1515:1551	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	6	66	theme	SAILs	1342:1346	arg1	complexes					1329:1337	The formed complexes	1318:1337	The formed complexes of SAILs and NaCMC	1318:1356	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	1	67	theme	chloride	354:361	arg1	[Cl					373:375	2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl	298:375	[Cl	373:375	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	67	theme	chloride	354:361	arg1	3-					295:296	3-	295:296	3-	295:296	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	8	68	theme	SAILs	1776:1780	arg1	nano-composites					1806:1820	SAILs and biopolymer assisted nano-composites	1776:1820	SAILs and biopolymer assisted nano-composites	1776:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	4	69	theme	various	971:977	arg1	microscopy					1080:1089	scanning electron microscopy	1062:1089	scanning electron microscopy (SEM)	1062:1095	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	measurements					1114:1125	fluorescence measurements	1101:1125	fluorescence measurements	1101:1125	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	conductivity					998:1009	conductivity	998:1009	conductivity	998:1009	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	scattering					1037:1046	dynamic light scattering	1023:1046	dynamic light scattering	1023:1046	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	ζ-potential					1049:1059	ζ-potential	1049:1059	ζ-potential	1049:1059	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	turbidity					1012:1020	turbidity	1012:1020	turbidity	1012:1020	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	4	69	theme	various	971:977	arg1	techniques					979:988	various techniques	971:988	various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements	971:1125	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	8	70	theme	biopolymer	1786:1795	arg1	nano-composites					1806:1820	SAILs and biopolymer assisted nano-composites	1776:1820	SAILs and biopolymer assisted nano-composites	1776:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	3	71	theme	concentration	925:937	arg1	regimes					939:945	different concentration regimes	915:945	different concentration regimes	915:945	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	0	72	theme	surface	21:27	arg1	liquids					42:48	surface active ionic liquids	21:48	surface active ionic liquids	21:48	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	8	73	theme	nano-composites	1806:1820	arg1	preparation					1761:1771	the preparation	1757:1771	the preparation of SAILs and biopolymer assisted nano-composites	1757:1820	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	7	74	theme	sample	1655:1660	arg1	VSM					1676:1678	VSM	1676:1678	VSM	1676:1678	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	7	74	theme	sample	1655:1660	arg1	magnetometry					1662:1673	vibrating sample magnetometry	1645:1673	vibrating sample magnetometry (VSM)	1645:1679	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	8	75	theme	present	1706:1712	arg1	work					1714:1717	the present work	1702:1717	the present work	1702:1717	It is expected that the present work would offer a new colloidal route for the preparation of SAILs and biopolymer assisted nano-composites along with providing physical insights into the complexation phenomenon.
29951672	5	76	theme	non-functionalized	1286:1303	arg1	counterpart					1305:1315	their non-functionalized counterpart	1280:1315	their non-functionalized counterpart	1280:1315	H-bonding prone SAILs, i.e. [C12Amim][Cl] and [C12Emim][Cl], are found to interact with NaCMC in a contrasting manner as compared to their non-functionalized counterpart.
29951672	0	77	theme	ionic	36:40	arg1	liquids					42:48	surface active ionic liquids	21:48	surface active ionic liquids	21:48	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	7	78	theme	prepared	1519:1526	arg1	nano-composites					1537:1551	The prepared magnetic nano-composites	1515:1551	The prepared magnetic nano-composites	1515:1551	The prepared magnetic nano-composites are characterised using X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM).
29951672	3	79	theme	SAILs	954:958	arg1	hydrophobicity/hydrophilicity					835:863	their relative hydrophobicity/hydrophilicity	820:863	their relative hydrophobicity/hydrophilicity	820:863	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	3	79	theme	SAILs	954:958	arg1	parameters					889:898	other thermodynamic parameters	869:898	other thermodynamic parameters of interest in different concentration regimes of the SAILs	869:958	The formed colloids in the bulk have been characterized for their mobility, surface charge, shape, size and morphology along with their relative hydrophobicity/hydrophilicity and other thermodynamic parameters of interest in different concentration regimes of the SAILs.
29951672	1	80	dep	liquids	209:215	arg1	amide					288:292	its amide	284:292	its amide	284:292	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	80	dep	liquids	209:215	arg1	[C12mim					266:272	[C12mim	266:272	[C12mim][Cl]	266:277	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	80	dep	liquids	209:215	arg1	chloride					256:263	1-methyl-3-dodecylimidazolium chloride	226:263	1-methyl-3-dodecylimidazolium chloride	226:263	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	80	dep	liquids	209:215	arg1	liquids					209:215	three surface active ionic liquids	182:215	three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl]	182:459	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	4	81	theme	dynamic	1023:1029	arg1	scattering					1037:1046	dynamic light scattering	1023:1046	dynamic light scattering	1023:1046	For this, various techniques such as conductivity, turbidity, dynamic light scattering, ζ-potential, scanning electron microscopy (SEM) and fluorescence measurements have been employed.
29951672	1	82	theme	sodium	495:500	arg1	NaCMC					526:530	NaCMC	526:530	NaCMC	526:530	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	1	82	theme	sodium	495:500	arg1	carboxymethylcellulose					502:523	sodium carboxymethylcellulose	495:523	sodium carboxymethylcellulose (NaCMC)	495:531	The complexation of three surface active ionic liquids (SAILs): 1-methyl-3-dodecylimidazolium chloride, [C12mim][Cl], and its amide, 3-(2-(dodecylamino)-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride, [C12Amim][Cl], and ester, 3-methyl-1-dodecyloxycarbonylmethylimidazolium chloride, [C12Emim][Cl], functionalized counterparts with sodium carboxymethylcellulose (NaCMC), has been investigated.
29951672	6	83	theme	zinc	1476:1479	arg1	nano-particles					1499:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	zinc ferrite (ZnFe3O4) nano-particles	1476:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	6	84	theme	NaCMC	1465:1469	arg1	colloids					1443:1450	doping colloids	1436:1450	doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles	1436:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29951672	0	85	dep	systems	10:16	arg1	investigations					102:115	physicochemical investigations	86:115	physicochemical investigations	86:115	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	0	85	dep	systems	10:16	arg1	preparation					121:131	preparation	121:131	preparation	121:131	Colloidal systems of surface active ionic liquids and sodium carboxymethyl cellulose: physicochemical investigations and preparation of magnetic nano-composites.
29951672	6	86	theme	SAILs	1455:1459	arg1	colloids					1443:1450	doping colloids	1436:1450	doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles	1436:1512	The formed complexes of SAILs and NaCMC have been explored for the one pot preparation of magnetic nano-composites by doping colloids of SAILs and NaCMC with zinc ferrite (ZnFe3O4) nano-particles.
29486264	4	0	theme	removal	470:476	arg1	isotherm					447:454	isotherm	447:454	isotherm	447:454	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	4	0	theme	removal	470:476	arg1	kinetics					434:441	kinetics	434:441	kinetics	434:441	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	7	1	theme	pollutants	987:996	arg1	removal					970:976	removal	970:976	removal of toxic pollutants from aqueous solutions	970:1019	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	4	2	dep	kinetics	434:441	arg1	The					430:432	The	430:432	The	430:432	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	0	3	theme	equilibrium	81:91	arg1	studies					93:99	Kinetics and equilibrium studies	68:99	Kinetics and equilibrium studies	68:99	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	3	4	theme	initial	340:346	arg1	concentration					352:364	initial dye concentration	340:364	initial dye concentration	340:364	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	4	5	theme	pseudo-second-order	485:503	arg1	kinetics					505:512	pseudo-second-order kinetics	485:512	pseudo-second-order kinetics	485:512	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	5	6	theme	maximum	596:602	arg1	capacity					615:622	a maximum adsorption capacity	594:622	a maximum adsorption capacity of 584.0 mg/g	594:636	The Langmiur isotherm model exhibted a maximum adsorption capacity of 584.0 mg/g.
29486264	2	7	theme	FTIR	291:294	arg1	techniques					309:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	The adsorbent was characterized using SEM, XRD, FTIR, and TGA/DTA techniques.
29486264	3	8	theme	dye	348:350	arg1	concentration					352:364	initial dye concentration	340:364	initial dye concentration	340:364	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	5	9	theme	adsorption	604:613	arg1	capacity					615:622	a maximum adsorption capacity	594:622	a maximum adsorption capacity of 584.0 mg/g	594:636	The Langmiur isotherm model exhibted a maximum adsorption capacity of 584.0 mg/g.
29486264	6	10	theme	positive	719:726	arg1	value					728:732	positive value	719:732	positive value of ΔH°	719:739	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	3	11	theme	pH	336:337	arg1	effects					325:331	The effects	321:331	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal	321:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	2	12	theme	XRD	286:288	arg1	techniques					309:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	The adsorbent was characterized using SEM, XRD, FTIR, and TGA/DTA techniques.
29486264	6	13	from	nature	815:820	arg1	spontaneous					784:794	spontaneous	784:794	spontaneous	784:794	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	6	13	from	nature	815:820	arg1	processes					770:778	the adsorption processes	755:778	the adsorption processes	755:778	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	4	14	theme	tartrazine	459:468	arg1	removal					470:476	tartrazine removal	459:476	tartrazine removal	459:476	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	3	15	theme	temperature	385:395	arg1	effects					325:331	The effects	321:331	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal	321:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	5	16	theme	Langmiur	561:568	arg1	model					579:583	The Langmiur isotherm model	557:583	The Langmiur isotherm model	557:583	The Langmiur isotherm model exhibted a maximum adsorption capacity of 584.0 mg/g.
29486264	0	17	theme	Adsorption	0:9	arg1	removal					11:17	Adsorption removal	0:17	Adsorption removal of tartrazine by chitosan/polyaniline composite	0:65	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	6	18	theme	ΔG°	711:713	arg1	value					728:732	positive value	719:732	positive value of ΔH°	719:739	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	6	18	theme	ΔG°	711:713	arg1	values					701:706	the negative values	688:706	the negative values of ΔG°	688:713	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	1	19	theme	composite	181:189	arg1	chitosan/polyaniline					149:168	chitosan/polyaniline	149:168	chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions	149:240	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
29486264	1	19	theme	composite	181:189	arg1	Cht-PANI					171:178	Cht-PANI	171:178	Cht-PANI	171:178	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
29486264	3	20	theme	contact	367:373	arg1	time					375:378	contact time	367:378	contact time	367:378	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	7	21	from	solutions	1011:1019	arg1	removal					970:976	removal	970:976	removal of toxic pollutants from aqueous solutions	970:1019	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	5	22	theme	isotherm	570:577	arg1	model					579:583	The Langmiur isotherm model	557:583	The Langmiur isotherm model	557:583	The Langmiur isotherm model exhibted a maximum adsorption capacity of 584.0 mg/g.
29486264	0	23	dep	studies	93:99	arg1	removal					11:17	Adsorption removal	0:17	Adsorption removal of tartrazine by chitosan/polyaniline composite	0:65	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	3	24	theme	azo	400:402	arg1	removal					408:414	azo dye removal	400:414	azo dye removal	400:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	3	25	theme	time	375:378	arg1	effects					325:331	The effects	321:331	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal	321:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	2	26	theme	TGA/DTA	301:307	arg1	techniques					309:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	The adsorbent was characterized using SEM, XRD, FTIR, and TGA/DTA techniques.
29486264	3	27	theme	dye	404:406	arg1	removal					408:414	azo dye removal	400:414	azo dye removal	400:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	3	28	theme	concentration	352:364	arg1	effects					325:331	The effects	321:331	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal	321:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	1	29	theme	present	106:112	arg1	work					114:117	The present work	102:117	The present work	102:117	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
29486264	6	30	theme	negative	692:699	arg1	values					701:706	the negative values	688:706	the negative values of ΔG°	688:713	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	1	31	theme	tartrazine	204:213	arg1	dye					215:217	tartrazine dye	204:217	tartrazine dye	204:217	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
29486264	7	32	theme	aqueous	1003:1009	arg1	solutions					1011:1019	aqueous solutions	1003:1019	aqueous solutions	1003:1019	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	0	33	theme	chitosan/polyaniline	36:55	arg1	composite					57:65	chitosan/polyaniline composite	36:65	chitosan/polyaniline composite	36:65	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	6	34	from	spontaneous	784:794	arg1	nature					815:820	nature	815:820	nature	815:820	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	0	35	theme	tartrazine	22:31	arg1	removal					11:17	Adsorption removal	0:17	Adsorption removal of tartrazine by chitosan/polyaniline composite	0:65	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	1	36	theme	aqueous	224:230	arg1	solutions					232:240	aqueous solutions	224:240	aqueous solutions	224:240	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
29486264	7	37	theme	resulting	840:848	arg1	reusability					860:870	the resulting adsorbent reusability	836:870	the resulting adsorbent reusability	836:870	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	6	38	theme	adsorption	759:768	arg1	spontaneous					784:794	spontaneous	784:794	spontaneous	784:794	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	6	38	theme	adsorption	759:768	arg1	processes					770:778	the adsorption processes	755:778	the adsorption processes	755:778	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	5	39	theme	584.0 mg/g	627:636	arg1	capacity					615:622	a maximum adsorption capacity	594:622	a maximum adsorption capacity of 584.0 mg/g	594:636	The Langmiur isotherm model exhibted a maximum adsorption capacity of 584.0 mg/g.
29486264	4	40	theme	Freundlich	522:531	arg1	isotherm					533:540	the Freundlich isotherm	518:540	the Freundlich isotherm	518:540	The kinetics and isotherm of tartrazine removal follow pseudo-second-order kinetics and the Freundlich isotherm, respectively.
29486264	2	41	theme	SEM	281:283	arg1	techniques					309:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	SEM, XRD, FTIR, and TGA/DTA techniques	281:318	The adsorbent was characterized using SEM, XRD, FTIR, and TGA/DTA techniques.
29486264	3	42	from	effects	325:331	arg1	removal					408:414	azo dye removal	400:414	azo dye removal	400:414	The effects of pH, initial dye concentration, contact time, and temperature on azo dye removal were studied.
29486264	7	43	theme	promising	946:954	arg1	a					939:939	a	939:939	a very promising	939:954	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	7	44	theme	adsorbent	850:858	arg1	reusability					860:870	the resulting adsorbent reusability	836:870	the resulting adsorbent reusability	836:870	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	6	45	theme	ΔH°	737:739	arg1	value					728:732	positive value	719:732	positive value of ΔH°	719:739	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	6	45	theme	ΔH°	737:739	arg1	values					701:706	the negative values	688:706	the negative values of ΔG°	688:713	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	6	46	theme	thermodynamic	643:655	arg1	parameters					657:666	The thermodynamic parameters	639:666	The thermodynamic parameters	639:666	The thermodynamic parameters were calculated and the negative values of ΔG° and positive value of ΔH° indicate that the adsorption processes are spontaneous and endothermic in nature.
29486264	0	47	theme	Kinetics	68:75	arg1	studies					93:99	Kinetics and equilibrium studies	68:99	Kinetics and equilibrium studies	68:99	Adsorption removal of tartrazine by chitosan/polyaniline composite: Kinetics and equilibrium studies.
29486264	7	48	theme	toxic	981:985	arg1	pollutants					987:996	toxic pollutants	981:996	toxic pollutants	981:996	In addition, the resulting adsorbent reusability was demonstrated over four cycles, indicating that the Cht-PANI is a very promising adsorbent for removal of toxic pollutants from aqueous solutions.
29486264	1	49	theme	chitosan/polyaniline	149:168	arg1	performance					134:144	the performance	130:144	the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions	130:240	The present work focused on the performance of chitosan/polyaniline (Cht-PANI) composite for removing tartrazine dye from aqueous solutions.
30170217	3	0	theme	tertiary	614:621	arg1	structure					623:631	a "salad-bowl" shaped tertiary structure	592:631	a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8)	592:668	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	4	1	theme	sequence	736:743	arg1	identity					745:752	at best 40% sequence identity	724:752	at best 40% sequence identity with other homologous GH10 xylanase proteins	724:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	8	2	theme	xylan	1158:1162	arg1	hydrolysates					1164:1175	bifidogenic xylan hydrolysates	1146:1175	bifidogenic xylan hydrolysates	1146:1175	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	1	3	theme	microbial	245:253	arg1	metagenomes					255:265	the microbial metagenomes	241:265	the microbial metagenomes of black goat rumens	241:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	0	4	theme	family	67:72	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	0	4	theme	family	67:72	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	3	5	theme	salad-bowl	595:604	arg1	structure					623:631	a "salad-bowl" shaped tertiary structure	592:631	a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8)	592:668	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	4	6	theme	%	734:734	arg1	identity					745:752	at best 40% sequence identity	724:752	at best 40% sequence identity with other homologous GH10 xylanase proteins	724:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	8	7	theme	potential	1196:1204	arg1	applications					1206:1217	its potential applications	1192:1217	its potential applications	1192:1217	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	7	8	theme	hydrolytic	990:999	arg1	patterns					1001:1008	hydrolytic patterns	990:1008	hydrolytic patterns	990:1008	Substrate specificity and hydrolytic patterns implied that the KG42 xylanase functions as an endo-β-1,4-xylanase (EC 3.2.1.8).
30170217	3	9	theme	"	605:605	arg1	structure					623:631	a "salad-bowl" shaped tertiary structure	592:631	a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8)	592:668	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	2	10	attach	isolated	313:320	arg2	KG42					303:306	KG42	303:306	KG42	303:306	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	10	attach	isolated	313:320	arg2	gene					297:300	A novel gene	289:300	A novel gene	289:300	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	10	attach	isolated	313:320	arg1	clones					376:381	the 17 xylan-degrading metagenomic fosmid clones	334:381	the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens	334:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	10	attach	isolated	313:320	arg1	one					327:329	one	327:329	one	327:329	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	8	11	used	used	1118:1121	arg2	xylanase					1100:1107	The KG42 xylanase	1091:1107	The KG42 xylanase	1091:1107	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	1	12	theme	black	270:274	arg1	rumens					281:286	black goat rumens	270:286	black goat rumens	270:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	0	13	theme	black	93:97	arg1	library					130:136	the black goat rumen-derived metagenomic library	89:136	the black goat rumen-derived metagenomic library	89:136	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	3	14	theme	typical	636:642	arg1	α/β					651:653	a typical 8-fold α/β	634:653	a typical 8-fold α/β barrel (α/β)8	634:667	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	6	15	theme	temperature	905:915	arg1	ranges					917:922	pH and temperature ranges	898:922	pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively	898:961	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	6	16	theme	5.0-10.0	927:934	arg1	ranges					917:922	pH and temperature ranges	898:922	pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively	898:961	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	2	17	theme	clones	376:381	arg1	clones					376:381	the 17 xylan-degrading metagenomic fosmid clones	334:381	the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens	334:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	17	theme	clones	376:381	arg1	one					327:329	one	327:329	one	327:329	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	0	18	theme	KG42	47:50	arg1	family					67:72	a novel KG42 xylanase (GH10 family)	39:73	a novel KG42 xylanase (GH10 family)	39:73	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	7	19	theme	Substrate	964:972	arg1	specificity					974:984	Substrate specificity	964:984	Substrate specificity	964:984	Substrate specificity and hydrolytic patterns implied that the KG42 xylanase functions as an endo-β-1,4-xylanase (EC 3.2.1.8).
30170217	8	20	theme	prebiotic	1236:1244	arg1	xylooligosaccharides					1246:1265	prebiotic xylooligosaccharides	1236:1265	prebiotic xylooligosaccharides	1236:1265	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	0	21	link	rumen-derived	104:116	arg1	library					130:136	the black goat rumen-derived metagenomic library	89:136	the black goat rumen-derived metagenomic library	89:136	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	2	22	theme	fosmid	369:374	arg1	clones					376:381	the 17 xylan-degrading metagenomic fosmid clones	334:381	the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens	334:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	5	23	theme	optimum	825:831	arg1	activity					833:840	its optimum activity	821:840	its optimum activity	821:840	The enzyme displayed its optimum activity at pH 5.0 and 50 °C.
30170217	2	24	theme	metagenomic	357:367	arg1	clones					376:381	the 17 xylan-degrading metagenomic fosmid clones	334:381	the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens	334:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	3	25	with	acids	512:516	arg1	domain					570:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	1	26	theme	goat	276:279	arg1	rumens					281:286	black goat rumens	270:286	black goat rumens	270:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	6	27	from	ranges	917:922	arg1	enzyme					867:872	The enzyme	863:872	The enzyme	863:872	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	6	27	from	ranges	917:922	arg1	stable					888:893	stable	888:893	stable	888:893	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	2	28	theme	xylan-degrading	341:355	arg1	clones					376:381	the 17 xylan-degrading metagenomic fosmid clones	334:381	the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens	334:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	1	29	theme	rumens	281:286	arg1	metagenomes					255:265	the microbial metagenomes	241:265	the microbial metagenomes of black goat rumens	241:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	0	30	theme	rumen-derived	104:116	arg1	library					130:136	the black goat rumen-derived metagenomic library	89:136	the black goat rumen-derived metagenomic library	89:136	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	3	31	theme	amino	506:510	arg1	acids					512:516	368 amino acids	502:516	368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues	502:695	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	31	theme	amino	506:510	arg1	41 kDa					519:524	41 kDa	519:524	41 kDa	519:524	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	2	32	theme	goat	403:406	arg1	rumens					408:413	black goat rumens	397:413	black goat rumens	397:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	3	33	theme	8-fold	644:649	arg1	α/β					651:653	a typical 8-fold α/β	634:653	a typical 8-fold α/β barrel (α/β)8	634:667	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	0	34	theme	goat	99:102	arg1	library					130:136	the black goat rumen-derived metagenomic library	89:136	the black goat rumen-derived metagenomic library	89:136	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	2	35	theme	novel	291:295	arg1	gene					297:300	A novel gene	289:300	A novel gene	289:300	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	35	theme	novel	291:295	arg1	KG42					303:306	KG42	303:306	KG42	303:306	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	2	36	theme	black	397:401	arg1	rumens					408:413	black goat rumens	397:413	black goat rumens	397:413	A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens.
30170217	0	37	theme	novel	41:45	arg1	family					67:72	a novel KG42 xylanase (GH10 family)	39:73	a novel KG42 xylanase (GH10 family)	39:73	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	8	38	theme	bifidogenic	1146:1156	arg1	hydrolysates					1164:1175	bifidogenic xylan hydrolysates	1146:1175	bifidogenic xylan hydrolysates	1146:1175	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	4	39	theme	KG42	698:701	arg1	protein					712:718	KG42 xylanase protein	698:718	KG42 xylanase protein	698:718	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	6	40	theme	20-60 °C	940:947	arg1	ranges					917:922	pH and temperature ranges	898:922	pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively	898:961	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	3	41	dep	structure	623:631	arg1	α/β					651:653	a typical 8-fold α/β	634:653	a typical 8-fold α/β barrel (α/β)8	634:667	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	1	42	from	metagenomes	255:265	arg1	enzyme					229:234	a novel xylan-degrading enzyme	205:234	a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens	205:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	3	43	theme	GH10	564:567	arg1	domain					570:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	44	theme	glycosyl	534:541	arg1	domain					570:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	8	45	theme	hydrolysates	1164:1175	arg1	preparation					1131:1141	the preparation	1127:1141	the preparation of bifidogenic xylan hydrolysates	1127:1175	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	1	46	theme	novel	207:211	arg1	enzyme					229:234	a novel xylan-degrading enzyme	205:234	a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens	205:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	0	47	theme	xylanase	52:59	arg1	family					67:72	a novel KG42 xylanase (GH10 family)	39:73	a novel KG42 xylanase (GH10 family)	39:73	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	4	48	theme	homologous	765:774	arg1	proteins					790:797	other homologous GH10 xylanase proteins	759:797	other homologous GH10 xylanase proteins	759:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	3	49	theme	open	452:455	arg1	frame					465:469	a 1107 bp open reading frame	442:469	a 1107 bp open reading frame	442:469	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	0	50	theme	metagenomic	118:128	arg1	library					130:136	the black goat rumen-derived metagenomic library	89:136	the black goat rumen-derived metagenomic library	89:136	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	4	51	theme	other	759:763	arg1	proteins					790:797	other homologous GH10 xylanase proteins	759:797	other homologous GH10 xylanase proteins	759:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	4	52	theme	xylanase	703:710	arg1	protein					712:718	KG42 xylanase protein	698:718	KG42 xylanase protein	698:718	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	3	53	dep	α/β	651:653	arg1	barrel					655:660	barrel (α/β)8	655:667	a typical 8-fold α/β barrel (α/β)8	634:667	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	54	theme	reading	457:463	arg1	frame					465:469	a 1107 bp open reading frame	442:469	a 1107 bp open reading frame	442:469	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	4	55	theme	xylanase	781:788	arg1	proteins					790:797	other homologous GH10 xylanase proteins	759:797	other homologous GH10 xylanase proteins	759:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	4	56	with	identity	745:752	arg1	proteins					790:797	other homologous GH10 xylanase proteins	759:797	other homologous GH10 xylanase proteins	759:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	1	57	theme	xylan-degrading	213:227	arg1	enzyme					229:234	a novel xylan-degrading enzyme	205:234	a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens	205:286	This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.
30170217	4	58	theme	GH10	776:779	arg1	proteins					790:797	other homologous GH10 xylanase proteins	759:797	other homologous GH10 xylanase proteins	759:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	3	59	theme	bp	449:450	arg1	frame					465:469	a 1107 bp open reading frame	442:469	a 1107 bp open reading frame	442:469	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	4	60	contain	has	720:722	arg2	identity					745:752	at best 40% sequence identity	724:752	at best 40% sequence identity with other homologous GH10 xylanase proteins	724:797	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	4	60	contain	has	720:722	arg1	protein					712:718	KG42 xylanase protein	698:718	KG42 xylanase protein	698:718	KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins.
30170217	0	61	theme	GH10	62:65	arg1	family					67:72	a novel KG42 xylanase (GH10 family)	39:73	a novel KG42 xylanase (GH10 family)	39:73	Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
30170217	6	62	theme	pH	898:899	arg1	ranges					917:922	pH and temperature ranges	898:922	pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively	898:961	The enzyme was thermally stable at pH and temperature ranges of 5.0-10.0 and 20-60 °C, respectively.
30170217	3	63	theme	hydrolase	543:551	arg1	domain					570:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	64	theme	KG42	420:423	arg1	protein					482:488	a protein	480:488	a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues	480:695	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	64	theme	KG42	420:423	arg1	gene					425:428	The KG42 gene	416:428	The KG42 gene	416:428	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	8	65	theme	KG42	1095:1098	arg1	xylanase					1100:1107	The KG42 xylanase	1091:1107	The KG42 xylanase	1091:1107	The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.
30170217	3	66	theme	shaped	607:612	arg1	structure					623:631	a "salad-bowl" shaped tertiary structure	592:631	a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8)	592:668	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	67	theme	family	553:558	arg1	domain					570:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	a glycosyl hydrolase family 10 (GH10) domain	532:575	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30170217	3	68	theme	catalytic	678:686	arg1	residues					688:695	two catalytic residues	674:695	two catalytic residues	674:695	The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a "salad-bowl" shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.
30274064	0	0	theme	polyurethane	117:128	arg1	membrane					130:137	functionalized polyurethane membrane	102:137	functionalized polyurethane membrane	102:137	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	1	1	theme	fibrous	378:384	arg1	surface					395:401	the fibrous membrane surface	374:401	the fibrous membrane surface	374:401	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	2	2	theme	Surface	607:613	arg1	composition					615:625	Surface composition	607:625	Surface composition	607:625	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	1	3	theme	functionalized	544:557	arg1	control					508:514	a control	506:514	a control	506:514	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	3	theme	functionalized	544:557	arg1	C-D-PU					580:585	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	4	theme	fibrous	288:294	arg1	membrane					296:303	the electrospinning polyurethane (PU) fibrous membrane	250:303	the electrospinning polyurethane (PU) fibrous membrane	250:303	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	0	5	theme	functionalized	102:115	arg1	membrane					130:137	functionalized polyurethane membrane	102:137	functionalized polyurethane membrane	102:137	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	1	6	theme	membrane	386:393	arg1	surface					395:401	the fibrous membrane surface	374:401	the fibrous membrane surface	374:401	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	3	7	theme	fibrous	1081:1087	arg1	membranes					1089:1097	pure PU and C-D-PU fibrous membranes	1062:1097	membranes	1089:1097	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	1	8	theme	G-COS	467:471	arg1	PU					488:489	the G-COS functionalized PU	463:489	the G-COS functionalized PU (G-C-D-PU)	463:500	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	8	theme	G-COS	467:471	arg1	G-C-D-PU					492:499	G-C-D-PU	492:499	G-C-D-PU	492:499	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	9	theme	membrane	296:303	arg1	activity					216:223	the antibacterial activity	198:223	the antibacterial activity	198:223	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	9	theme	membrane	296:303	arg1	cytocompatibility					229:245	cytocompatibility	229:245	cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane	229:303	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	3	10	theme	pure	1062:1065	arg1	PU					1067:1068	pure PU and C-D-PU fibrous membranes	1062:1097	PU	1067:1068	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	1	11	theme	functionalized	473:486	arg1	PU					488:489	the G-COS functionalized PU	463:489	the G-COS functionalized PU (G-C-D-PU)	463:500	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	11	theme	functionalized	473:486	arg1	G-C-D-PU					492:499	G-C-D-PU	492:499	G-C-D-PU	492:499	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	4	12	theme	NIH-3T3	1183:1189	arg1	cells					1191:1195	NIH-3T3 cells	1183:1195	NIH-3T3 cells	1183:1195	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	2	13	dep	roughness	776:784	arg1	the					764:766	the	764:766	the	764:766	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	3	14	theme	membrane	1020:1027	arg1	activity					987:994	antibacterial activity	973:994	antibacterial activity of the G-C-D-PU fibrous membrane	973:1027	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	1	15	theme	PU	559:560	arg1	control					508:514	a control	506:514	a control	506:514	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	15	theme	PU	559:560	arg1	C-D-PU					580:585	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	16	theme	quaternary	306:315	arg1	G-COS					348:352	G-COS	348:352	G-COS	348:352	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	16	theme	quaternary	306:315	arg1	chitooligosaccharide					326:345	quaternary ammonium chitooligosaccharide	306:345	quaternary ammonium chitooligosaccharide (G-COS)	306:353	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	0	17	theme	adhesive	156:163	arg1	layer					165:169	polydopamine adhesive layer	143:169	polydopamine adhesive layer	143:169	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	2	18	theme	surface	768:774	arg1	roughness					776:784	surface roughness	768:784	surface roughness	768:784	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	3	19	theme	C-D-PU	1074:1079	arg1	membranes					1089:1097	pure PU and C-D-PU fibrous membranes	1062:1097	membranes	1089:1097	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	1	20	theme	fibrous	562:568	arg1	control					508:514	a control	506:514	a control	506:514	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	20	theme	fibrous	562:568	arg1	C-D-PU					580:585	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	0	21	theme	polydopamine	143:154	arg1	layer					165:169	polydopamine adhesive layer	143:169	polydopamine adhesive layer	143:169	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	1	22	theme	intermediate	410:421	arg1	layer					423:427	an intermediate layer	407:427	an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU)	407:500	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	5	23	theme	activity	1476:1483	arg1	enhancement					1447:1457	the enhancement	1443:1457	the enhancement of antibacterial activity	1443:1483	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	24	with	membranes	1376:1384	arg1	content					1411:1417	relative high G-COS content	1391:1417	relative high G-COS content	1391:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	1	25	theme	membrane	570:577	arg1	control					508:514	a control	506:514	a control	506:514	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	25	theme	membrane	570:577	arg1	C-D-PU					580:585	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU)	517:586	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	26	theme	antibacterial	202:214	arg1	activity					216:223	the antibacterial activity	198:223	the antibacterial activity	198:223	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	4	27	theme	2 g/L	1328:1332	arg1	concentration					1311:1323	the concentration	1307:1323	the concentration of 2 g/L and 6 g/L	1307:1342	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	2	28	theme	original	681:688	arg1	membranes					714:722	the original and modified PU fibrous membranes	677:722	the original and modified PU fibrous membranes	677:722	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	5	29	theme	antibacterial	1462:1474	arg1	activity					1476:1483	antibacterial activity	1462:1483	antibacterial activity	1462:1483	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	1	30	theme	ammonium	317:324	arg1	G-COS					348:352	G-COS	348:352	G-COS	348:352	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	1	30	theme	ammonium	317:324	arg1	chitooligosaccharide					326:345	quaternary ammonium chitooligosaccharide	306:345	quaternary ammonium chitooligosaccharide (G-COS)	306:353	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	5	31	theme	G-C-D-PU	1359:1366	arg1	membranes					1376:1384	the G-C-D-PU fibrous membranes	1355:1384	the G-C-D-PU fibrous membranes with relative high G-COS content	1355:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	31	theme	G-C-D-PU	1359:1366	arg1	beneficial					1429:1438	beneficial	1429:1438	beneficial	1429:1438	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	4	32	from	adhesion	1153:1160	arg1	membrane					1215:1222	the PU fibrous membrane	1200:1222	the PU fibrous membrane	1200:1222	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	4	33	theme	culture	1115:1121	arg1	experiments					1123:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	5	34	theme	G-COS	1531:1535	arg1	content					1537:1543	relative low G-COS content	1518:1543	relative low G-COS content	1518:1543	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	4	35	theme	cells	1109:1113	arg1	experiments					1123:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	0	36	theme	antibacterial	13:25	arg1	activity					27:34	antibacterial activity	13:34	antibacterial activity	13:34	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	4	37	theme	fibrous	1207:1213	arg1	membrane					1215:1222	the PU fibrous membrane	1200:1222	the PU fibrous membrane	1200:1222	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	1	38	theme	polydopamine	432:443	arg1	layer					423:427	an intermediate layer	407:427	an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU)	407:500	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	5	39	theme	relative	1391:1398	arg1	content					1411:1417	relative high G-COS content	1391:1417	relative high G-COS content	1391:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	40	theme	fibrous	1368:1374	arg1	membranes					1376:1384	the G-C-D-PU fibrous membranes	1355:1384	the G-C-D-PU fibrous membranes with relative high G-COS content	1355:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	40	theme	fibrous	1368:1374	arg1	beneficial					1429:1438	beneficial	1429:1438	beneficial	1429:1438	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	41	theme	low	1527:1529	arg1	content					1537:1543	relative low G-COS content	1518:1543	relative low G-COS content	1518:1543	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	2	42	theme	membranes	714:722	arg1	hydrophilicity					640:653	hydrophilicity	640:653	hydrophilicity	640:653	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	42	theme	membranes	714:722	arg1	energy					667:672	surface energy	659:672	surface energy	659:672	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	42	theme	membranes	714:722	arg1	morphology					628:637	morphology	628:637	morphology	628:637	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	42	theme	membranes	714:722	arg1	composition					615:625	Surface composition	607:625	Surface composition	607:625	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	1	43	theme	electrospinning	254:268	arg1	membrane					296:303	the electrospinning polyurethane (PU) fibrous membrane	250:303	the electrospinning polyurethane (PU) fibrous membrane	250:303	In this study, to enhance the antibacterial activity and cytocompatibility of the electrospinning polyurethane (PU) fibrous membrane, quaternary ammonium chitooligosaccharide (G-COS) was immobilized on the fibrous membrane surface via an intermediate layer of polydopamine (PDOPA) to obtain the G-COS functionalized PU (G-C-D-PU), as a control, chitooligosaccharide (COS) functionalized PU fibrous membrane (C-D-PU) was prepared, too.
30274064	0	44	theme	quaternary	61:70	arg1	ammonium					72:79	quaternary ammonium	61:79	quaternary ammonium	61:79	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	4	45	dep	adhesion	1153:1160	arg1	the					1149:1151	the	1149:1151	the	1149:1151	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	2	46	theme	fibrous	706:712	arg1	membranes					714:722	the original and modified PU fibrous membranes	677:722	the original and modified PU fibrous membranes	677:722	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	4	47	theme	PU	1204:1205	arg1	membrane					1215:1222	the PU fibrous membrane	1200:1222	the PU fibrous membrane	1200:1222	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	5	48	dep	cell	1567:1570	arg1	attachment					1572:1581	attachment	1572:1581	attachment	1572:1581	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	2	49	theme	PU	703:704	arg1	membranes					714:722	the original and modified PU fibrous membranes	677:722	the original and modified PU fibrous membranes	677:722	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	50	theme	membrane	823:830	arg1	hydrophilicity					790:803	hydrophilicity	790:803	hydrophilicity	790:803	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	50	theme	membrane	823:830	arg1	roughness					776:784	surface roughness	768:784	surface roughness	768:784	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	5	51	theme	relative	1518:1525	arg1	content					1537:1543	relative low G-COS content	1518:1543	relative low G-COS content	1518:1543	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	5	52	theme	high	1400:1403	arg1	content					1411:1417	relative high G-COS content	1391:1417	relative high G-COS content	1391:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
30274064	2	53	theme	surface	659:665	arg1	energy					667:672	surface energy	659:672	surface energy	659:672	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	54	theme	fibrous	815:821	arg1	membrane					823:830	the PU fibrous membrane	808:830	the PU fibrous membrane	808:830	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	2	55	theme	modified	694:701	arg1	membranes					714:722	the original and modified PU fibrous membranes	677:722	the original and modified PU fibrous membranes	677:722	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	3	56	theme	antibacterial	973:985	arg1	activity					987:994	antibacterial activity	973:994	antibacterial activity of the G-C-D-PU fibrous membrane	973:1027	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	3	57	theme	G-C-D-PU	1003:1010	arg1	membrane					1020:1027	the G-C-D-PU fibrous membrane	999:1027	the G-C-D-PU fibrous membrane	999:1027	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	2	58	theme	PU	812:813	arg1	membrane					823:830	the PU fibrous membrane	808:830	the PU fibrous membrane	808:830	Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU fibrous membranes were characterized, which revealed that the surface roughness and hydrophilicity of the PU fibrous membrane were obviously increased by modified with COS and G-COS, respectively.
30274064	3	59	theme	Antibacterial	903:915	arg1	experiment					917:926	Antibacterial experiment	903:926	Antibacterial experiment against E. coli and S. aureus	903:956	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	4	60	dep	In	1100:1101	arg1	vitro					1103:1107	vitro	1103:1107	vitro	1103:1107	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	3	61	theme	fibrous	1012:1018	arg1	membrane					1020:1027	the G-C-D-PU fibrous membrane	999:1027	the G-C-D-PU fibrous membrane	999:1027	Antibacterial experiment against E. coli and S. aureus indicated that antibacterial activity of the G-C-D-PU fibrous membrane was markedly superior to that of pure PU and C-D-PU fibrous membranes.
30274064	4	62	theme	In	1100:1101	arg1	experiments					1123:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments	1100:1133	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	4	63	theme	6 g/L	1338:1342	arg1	concentration					1311:1323	the concentration	1307:1323	the concentration of 2 g/L and 6 g/L	1307:1342	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	0	64	theme	ammonium	72:79	arg1	cytocompatibility					40:56	cytocompatibility	40:56	cytocompatibility	40:56	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	0	64	theme	ammonium	72:79	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	0	64	theme	ammonium	72:79	arg1	activity					27:34	antibacterial activity	13:34	antibacterial activity	13:34	Fabrication, antibacterial activity and cytocompatibility of quaternary ammonium chitooligosaccharide functionalized polyurethane membrane via polydopamine adhesive layer.
30274064	4	65	from	proliferation	1166:1178	arg1	membrane					1215:1222	the PU fibrous membrane	1200:1222	the PU fibrous membrane	1200:1222	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	4	66	theme	cells	1191:1195	arg1	proliferation					1166:1178	proliferation	1166:1178	proliferation	1166:1178	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	4	66	theme	cells	1191:1195	arg1	adhesion					1153:1160	adhesion	1153:1160	adhesion	1153:1160	In vitro cells culture experiments revealed that the adhesion and proliferation of NIH-3T3 cells on the PU fibrous membrane were improved by successively immobilized with PDOPA and COS as well as G-COS with the concentration of 2 g/L and 6 g/L.
30274064	5	67	theme	G-COS	1405:1409	arg1	content					1411:1417	relative high G-COS content	1391:1417	relative high G-COS content	1391:1417	Moreover, the G-C-D-PU fibrous membranes with relative high G-COS content were more beneficial to the enhancement of antibacterial activity, but on the contrary, those with relative low G-COS content were more in favor of cell attachment and proliferation.
31005573	0	0	theme	pacinian	73:80	arg1	corpuscles					82:91	human cutaneous pacinian corpuscles	57:91	human cutaneous pacinian corpuscles	57:91	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	1	1	theme	cells	329:333	arg1	structure					194:202	a complicated structure	180:202	a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	180:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	4	2	theme	leucine-rich	856:867	arg1	proteoglycans					869:881	small leucine-rich proteoglycans	850:881	small leucine-rich proteoglycans	850:881	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	3	3	theme	matrix	636:641	arg1	assembly					643:650	extracellular matrix assembly	622:650	extracellular matrix assembly	622:650	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	0	4	theme	cutaneous	63:71	arg1	corpuscles					82:91	human cutaneous pacinian corpuscles	57:91	human cutaneous pacinian corpuscles	57:91	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	4	5	theme	small	850:854	arg1	proteoglycans					869:881	small leucine-rich proteoglycans	850:881	small leucine-rich proteoglycans	850:881	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	3	6	theme	major	506:510	arg1	proteoglycans					488:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	3	6	theme	major	506:510	arg1	proteoglycans					512:524	major proteoglycans	506:524	major proteoglycans of the extracellular matrix	506:552	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	5	7	theme	outer	1113:1117	arg1	biglycan					1124:1131	the outer core biglycan	1109:1131	the outer core biglycan	1109:1131	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	0	8	from	Class	0:4	arg1	corpuscles					82:91	human cutaneous pacinian corpuscles	57:91	human cutaneous pacinian corpuscles	57:91	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	5	9	theme	core	1119:1122	arg1	biglycan					1124:1131	the outer core biglycan	1109:1131	the outer core biglycan	1109:1131	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	3	10	theme	matrix	547:552	arg1	proteoglycans					488:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	3	10	theme	matrix	547:552	arg1	proteoglycans					512:524	major proteoglycans	506:524	major proteoglycans of the extracellular matrix	506:552	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	7	11	theme	human	1407:1411	arg1	corpuscles					1422:1431	human Pacinian corpuscles	1407:1431	human Pacinian corpuscles	1407:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	4	12	dep	class	778:782	arg1	osteoadherin					836:847	osteoadherin	836:847	osteoadherin	836:847	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	12	dep	class	778:782	arg1	fibromodulin					788:799	fibromodulin	788:799	fibromodulin	788:799	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	12	dep	class	778:782	arg1	keratocan					822:830	keratocan	822:830	keratocan	822:830	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	12	dep	class	778:782	arg1	prolargin					811:819	prolargin	811:819	prolargin	811:819	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	12	dep	class	778:782	arg1	lumican					802:808	lumican	802:808	lumican	802:808	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	5	13	theme	inner	968:972	arg1	core					974:977	the inner core	964:977	the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule	964:1197	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	4	14	from	distribution	706:717	arg1	corpuscles					911:920	human cutaneous Pacinian corpuscles	886:920	human cutaneous Pacinian corpuscles	886:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	1	15	theme	endoneural	259:268	arg1	cells					278:282	endoneural related cells	259:282	endoneural related cells (intermediate layer)	259:303	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	15	theme	endoneural	259:268	arg1	layer					298:302	intermediate layer	285:302	intermediate layer	285:302	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	5	16	dep	core	974:977	arg1	express					979:985	express	979:985	express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule	979:1197	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	4	17	theme	class	778:782	arg1	distribution					706:717	the distribution	702:717	the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles	702:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	7	18	from	proteoglycans	1390:1402	arg1	corpuscles					1422:1431	human Pacinian corpuscles	1407:1431	human Pacinian corpuscles	1407:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	5	19	contain	contains	1199:1206	arg2	fibromodulin					1227:1238	fibromodulin	1227:1238	fibromodulin	1227:1238	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	5	19	contain	contains	1199:1206	arg2	biglycan					1208:1215	biglycan	1208:1215	biglycan	1208:1215	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	5	19	contain	contains	1199:1206	arg2	decorin					1218:1224	decorin	1218:1224	decorin	1218:1224	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	5	19	contain	contains	1199:1206	arg1	core					974:977	the inner core	964:977	the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule	964:1197	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	5	19	contain	contains	1199:1206	arg2	lumican					1245:1251	lumican	1245:1251	lumican	1245:1251	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	3	20	theme	extracellular	533:545	arg1	matrix					547:552	the extracellular matrix	529:552	the extracellular matrix	529:552	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	1	21	theme	complicated	182:192	arg1	structure					194:202	a complicated structure	180:202	a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	180:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	2	22	theme	that	437:440	arg1	properties					423:432	the properties	419:432	the properties of that covering the nerve fibers	419:466	The cells forming those compartments are continuous and share the properties of that covering the nerve fibers.
31005573	1	23	dep	cells	329:333	arg1	core-capsule					342:353	core-capsule	342:353	core-capsule	342:353	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	7	24	theme	matrix	1494:1499	arg1	composition					1461:1471	the composition	1457:1471	the composition of the extracellular matrix in these sensory formations	1457:1527	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	7	25	theme	Pacinian	1413:1420	arg1	corpuscles					1422:1431	human Pacinian corpuscles	1407:1431	human Pacinian corpuscles	1407:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	4	26	theme	human	886:890	arg1	corpuscles					911:920	human cutaneous Pacinian corpuscles	886:920	human cutaneous Pacinian corpuscles	886:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	1	27	theme	related	270:276	arg1	cells					278:282	endoneural related cells	259:282	endoneural related cells (intermediate layer)	259:303	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	27	theme	related	270:276	arg1	layer					298:302	intermediate layer	285:302	intermediate layer	285:302	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	7	28	theme	sensory	1510:1516	arg1	formations					1518:1527	these sensory formations	1504:1527	these sensory formations	1504:1527	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	5	29	theme	compounds	949:957	arg1	distribution					927:938	The distribution	923:938	The distribution of these compounds	923:957	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	7	30	from	composition	1461:1471	arg1	formations					1518:1527	these sensory formations	1504:1527	these sensory formations	1504:1527	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	1	31	theme	cells	278:282	arg1	structure					194:202	a complicated structure	180:202	a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	180:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	7	32	theme	small	1371:1375	arg1	proteoglycans					1390:1402	small leucine-rich proteoglycans	1371:1402	small leucine-rich proteoglycans in human Pacinian corpuscles	1371:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	1	33	theme	Pacinian	94:101	arg1	mechanoreceptors					147:162	onion bulb-like multilayered mechanoreceptors	118:162	onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	118:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	33	theme	Pacinian	94:101	arg1	corpuscles					103:112	Pacinian corpuscles	94:112	Pacinian corpuscles	94:112	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	34	theme	axon	207:210	arg1	terminals					212:220	axon terminals	207:220	axon terminals	207:220	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	4	35	dep	class	722:726	arg1	decorin					741:747	decorin	741:747	decorin	741:747	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	35	dep	class	722:726	arg1	ECMX					768:771	ECMX	768:771	ECMX	768:771	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	35	dep	class	722:726	arg1	asporin					750:756	asporin	750:756	asporin	750:756	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	35	dep	class	722:726	arg1	ECM2					759:762	ECM2	759:762	ECM2	759:762	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	35	dep	class	722:726	arg1	proteoglycans					869:881	small leucine-rich proteoglycans	850:881	small leucine-rich proteoglycans	850:881	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	35	dep	class	722:726	arg1	biglycan					731:738	biglycan	731:738	biglycan	731:738	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	5	36	theme	layer	1060:1064	arg1	immunoreactivity					1074:1089	the intermediate layer display immunoreactivity	1043:1089	the intermediate layer display immunoreactivity for osteoadherin	1043:1106	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	3	37	theme	collagen	567:574	arg1	fibrillogenesis					576:590	collagen fibrillogenesis	567:590	collagen fibrillogenesis	567:590	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	1	38	theme	terminals	212:220	arg1	structure					194:202	a complicated structure	180:202	a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	180:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	0	39	theme	small	21:25	arg1	proteoglycans					40:52	small leucine-rich proteoglycans	21:52	small leucine-rich proteoglycans	21:52	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	1	40	theme	intermediate	285:296	arg1	cells					278:282	endoneural related cells	259:282	endoneural related cells (intermediate layer)	259:303	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	40	theme	intermediate	285:296	arg1	layer					298:302	intermediate layer	285:302	intermediate layer	285:302	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	5	41	theme	display	1066:1072	arg1	immunoreactivity					1074:1089	the intermediate layer display immunoreactivity	1043:1089	the intermediate layer display immunoreactivity for osteoadherin	1043:1106	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	7	42	theme	leucine-rich	1377:1388	arg1	proteoglycans					1390:1402	small leucine-rich proteoglycans	1371:1402	small leucine-rich proteoglycans in human Pacinian corpuscles	1371:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	7	43	from	corpuscles	1422:1431	arg1	distribution					1355:1366	the distribution	1351:1366	the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles	1351:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	2	44	theme	nerve	455:459	arg1	fibers					461:466	the nerve fibers	451:466	the nerve fibers	451:466	The cells forming those compartments are continuous and share the properties of that covering the nerve fibers.
31005573	7	45	theme	proteoglycans	1390:1402	arg1	distribution					1355:1366	the distribution	1351:1366	the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles	1351:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	1	46	theme	Schwann	223:229	arg1	cells					239:243	Schwann related cells	223:243	Schwann related cells (inner core)	223:256	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	46	theme	Schwann	223:229	arg1	core					252:255	inner core	246:255	inner core	246:255	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	7	47	from	distribution	1355:1366	arg1	corpuscles					1422:1431	human Pacinian corpuscles	1407:1431	human Pacinian corpuscles	1407:1431	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	4	48	theme	cutaneous	892:900	arg1	corpuscles					911:920	human cutaneous Pacinian corpuscles	886:920	human cutaneous Pacinian corpuscles	886:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	3	49	theme	cell	593:596	arg1	pathways					609:616	cell signalling pathways	593:616	cell signalling pathways	593:616	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	3	50	theme	leucine-rich	475:486	arg1	proteoglycans					488:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	3	50	theme	leucine-rich	475:486	arg1	proteoglycans					512:524	major proteoglycans	506:524	major proteoglycans of the extracellular matrix	506:552	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	0	51	theme	leucine-rich	27:38	arg1	proteoglycans					40:52	small leucine-rich proteoglycans	21:52	small leucine-rich proteoglycans	21:52	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	1	52	theme	related	321:327	arg1	cells					329:333	perineurial related cells	309:333	perineurial related cells (outer core-capsule)	309:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	3	53	theme	signalling	598:607	arg1	pathways					609:616	cell signalling pathways	593:616	cell signalling pathways	593:616	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	1	54	theme	onion	118:122	arg1	mechanoreceptors					147:162	onion bulb-like multilayered mechanoreceptors	118:162	onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	118:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	54	theme	onion	118:122	arg1	corpuscles					103:112	Pacinian corpuscles	94:112	Pacinian corpuscles	94:112	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	7	55	theme	extracellular	1480:1492	arg1	matrix					1494:1499	the extracellular matrix	1476:1499	the extracellular matrix	1476:1499	These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
31005573	1	56	theme	perineurial	309:319	arg1	cells					329:333	perineurial related cells	309:333	perineurial related cells (outer core-capsule)	309:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	57	theme	bulb-like	124:132	arg1	mechanoreceptors					147:162	onion bulb-like multilayered mechanoreceptors	118:162	onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	118:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	57	theme	bulb-like	124:132	arg1	corpuscles					103:112	Pacinian corpuscles	94:112	Pacinian corpuscles	94:112	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	58	theme	related	231:237	arg1	cells					239:243	Schwann related cells	223:243	Schwann related cells (inner core)	223:256	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	58	theme	related	231:237	arg1	core					252:255	inner core	246:255	inner core	246:255	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	4	59	theme	Pacinian	902:909	arg1	corpuscles					911:920	human cutaneous Pacinian corpuscles	886:920	human cutaneous Pacinian corpuscles	886:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	4	60	used	used	661:664	arg2	we					658:659	we	658:659	we	658:659	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	1	61	theme	multilayered	134:145	arg1	mechanoreceptors					147:162	onion bulb-like multilayered mechanoreceptors	118:162	onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	118:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	61	theme	multilayered	134:145	arg1	corpuscles					103:112	Pacinian corpuscles	94:112	Pacinian corpuscles	94:112	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	62	theme	cells	239:243	arg1	structure					194:202	a complicated structure	180:202	a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule)	180:354	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	0	63	theme	human	57:61	arg1	corpuscles					82:91	human cutaneous pacinian corpuscles	57:91	human cutaneous pacinian corpuscles	57:91	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	5	64	theme	intermediate	1047:1058	arg1	immunoreactivity					1074:1089	the intermediate layer display immunoreactivity	1043:1089	the intermediate layer display immunoreactivity for osteoadherin	1043:1106	The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican.
31005573	3	65	theme	extracellular	622:634	arg1	assembly					643:650	extracellular matrix assembly	622:650	extracellular matrix assembly	622:650	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	4	66	theme	class	722:726	arg1	distribution					706:717	the distribution	702:717	the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles	702:920	Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles.
31005573	3	67	theme	Small	469:473	arg1	proteoglycans					488:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans	469:500	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	3	67	theme	Small	469:473	arg1	proteoglycans					512:524	major proteoglycans	506:524	major proteoglycans of the extracellular matrix	506:552	Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly.
31005573	0	68	from	Class	12:16	arg1	corpuscles					82:91	human cutaneous pacinian corpuscles	57:91	human cutaneous pacinian corpuscles	57:91	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	0	69	dep	Class	0:4	arg1	proteoglycans					40:52	small leucine-rich proteoglycans	21:52	small leucine-rich proteoglycans	21:52	Class I and Class II small leucine-rich proteoglycans in human cutaneous pacinian corpuscles.
31005573	1	70	theme	inner	246:250	arg1	cells					239:243	Schwann related cells	223:243	Schwann related cells (inner core)	223:256	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
31005573	1	70	theme	inner	246:250	arg1	core					252:255	inner core	246:255	inner core	246:255	Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule).
32090961	6	0	theme	ceramics	1364:1371	arg1	group					1373:1377	the ceramics group	1360:1377	the ceramics group	1360:1377	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	4	1	theme	most	712:715	arg1	structures					735:744	most 3D chitosan-based structures	712:744	most 3D chitosan-based structures	712:744	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	2	theme	Berkovich	889:897	arg1	tests					899:903	Berkovich tests	889:903	Berkovich tests	889:903	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	3	theme	chitosan-based	720:733	arg1	structures					735:744	most 3D chitosan-based structures	712:744	most 3D chitosan-based structures	712:744	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	6	4	theme	HA+β-TCP	1443:1450	arg1	powder					1452:1457	10% HA+β-TCP powder	1439:1457	10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2)	1439:1527	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	1	5	theme	shielding	262:270	arg1	effect					272:277	the stress shielding effect	251:277	the stress shielding effect	251:277	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	8	6	theme	fixation	1763:1770	arg1	applications					1772:1783	orthopedic fixation applications	1752:1783	orthopedic fixation applications	1752:1783	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	6	7	theme	flexural	1278:1285	arg1	strength					1287:1294	flexural strength	1278:1294	flexural strength of 53.8 MPa	1278:1306	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	4	8	from	effect	802:807	arg1	specimens					847:855	the produced specimens	834:855	the produced specimens	834:855	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	9	theme	3D	717:718	arg1	structures					735:744	most 3D chitosan-based structures	712:744	most 3D chitosan-based structures	712:744	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	6	10	theme	%	1441:1441	arg1	powder					1452:1457	10% HA+β-TCP powder	1439:1457	10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2)	1439:1527	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	7	11	theme	material	1545:1552	arg1	condition					1607:1615	dense and homogeneous, fundamental condition	1572:1615	condition	1607:1615	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	7	11	theme	material	1545:1552	arg1	compositions					1554:1565	All the tested material compositions	1530:1565	All the tested material compositions	1530:1565	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	0	12	theme	orthopedic	95:104	arg1	applications					115:126	orthopedic fixation applications	95:126	orthopedic fixation applications	95:126	Strategy to improve the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications.
32090961	1	13	used	used	180:183	arg2	devices					162:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	13	used	used	180:183	arg2	alternative					191:201	an alternative	188:201	an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal	188:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	6	14	theme	10	1439:1440	arg1	%					1441:1441	%	1441:1441	%	1441:1441	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	15	theme	%	1266:1266	arg1	glycerol					1268:1275	10% glycerol	1264:1275	10% glycerol	1264:1275	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	8	16	theme	encouraging	1659:1669	arg1	These					1649:1653	These	1649:1653	These	1649:1653	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	8	16	theme	encouraging	1659:1669	arg1	results					1671:1677	encouraging results	1659:1677	encouraging results	1659:1677	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	5	17	theme	mechanical	987:996	arg1	properties					998:1007	the mechanical properties	983:1007	the mechanical properties	983:1007	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	8	18	theme	chitosan-based	1723:1736	arg1	materials					1738:1746	chitosan-based materials	1723:1746	chitosan-based materials for orthopedic fixation applications	1723:1783	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	0	19	theme	fixation	106:113	arg1	applications					115:126	orthopedic fixation applications	95:126	orthopedic fixation applications	95:126	Strategy to improve the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications.
32090961	6	20	theme	10	1264:1265	arg1	%					1266:1266	%	1266:1266	%	1266:1266	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	7	21	theme	tested	1538:1543	arg1	condition					1607:1615	dense and homogeneous, fundamental condition	1572:1615	condition	1607:1615	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	7	21	theme	tested	1538:1543	arg1	compositions					1554:1565	All the tested material compositions	1530:1565	All the tested material compositions	1530:1565	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	5	22	theme	properties	998:1007	arg1	improvement					968:978	The improvement	964:978	The improvement of the mechanical properties	964:1007	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	7	23	theme	homogeneous	1582:1592	arg1	condition					1607:1615	dense and homogeneous, fundamental condition	1572:1615	condition	1607:1615	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	7	23	theme	homogeneous	1582:1592	arg1	compositions					1554:1565	All the tested material compositions	1530:1565	All the tested material compositions	1530:1565	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	5	24	theme	beta-tricalcium	1123:1137	arg1	β-TCP					1150:1154	β-TCP	1150:1154	β-TCP	1150:1154	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	24	theme	beta-tricalcium	1123:1137	arg1	phosphate					1139:1147	beta-tricalcium phosphate	1123:1147	beta-tricalcium phosphate (β-TCP)	1123:1155	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	6	25	theme	plasticizers	1165:1176	arg1	group					1178:1182	the plasticizers group	1161:1182	the plasticizers group	1161:1182	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	8	26	theme	continued	1698:1706	arg1	development					1708:1718	the continued development	1694:1718	the continued development of chitosan-based materials for orthopedic fixation applications	1694:1783	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	7	27	theme	good	1623:1626	arg1	performance					1636:1646	a good implant performance	1621:1646	a good implant performance	1621:1646	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	4	28	theme	flexural	873:880	arg1	tests					882:886	flexural tests	873:886	flexural tests	873:886	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	6	29	theme	hardness	1506:1513	arg1	kgf/mm2					1520:1526	indentation hardness 28.2 kgf/mm2	1494:1526	indentation hardness 28.2 kgf/mm2	1494:1526	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	2	30	theme	bioabsorbable	390:402	arg1	implants					404:411	current bioabsorbable implants	382:411	current bioabsorbable implants	382:411	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	30	theme	bioabsorbable	390:402	arg1	strength					461:468	strength	461:468	strength	461:468	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	30	theme	bioabsorbable	390:402	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	1	31	theme	second	294:299	arg1	surgery					301:307	a second surgery	292:307	a second surgery for implant removal	292:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	6	32	theme	kgf/mm2	1341:1347	arg1	hardness					1324:1331	indentation hardness	1312:1331	indentation hardness of 19.4 kgf/mm2	1312:1347	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	32	theme	kgf/mm2	1341:1347	arg1	strength					1287:1294	flexural strength	1278:1294	flexural strength of 53.8 MPa	1278:1306	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	33	theme	indentation	1494:1504	arg1	kgf/mm2					1520:1526	indentation hardness 28.2 kgf/mm2	1494:1526	indentation hardness 28.2 kgf/mm2	1494:1526	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	1	34	theme	metallic	206:213	arg1	implants					215:222	metallic implants	206:222	metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal	206:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	8	35	theme	materials	1738:1746	arg1	development					1708:1718	the continued development	1694:1718	the continued development of chitosan-based materials for orthopedic fixation applications	1694:1783	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	6	36	theme	best	1189:1192	arg1	combination					1194:1204	the best combination	1185:1204	the best combination of the measured properties	1185:1231	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	37	theme	67.5 MPa	1481:1488	arg1	kgf/mm2					1520:1526	indentation hardness 28.2 kgf/mm2	1494:1526	indentation hardness 28.2 kgf/mm2	1494:1526	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	37	theme	67.5 MPa	1481:1488	arg1	strength					1469:1476	flexural strength	1460:1476	flexural strength of 67.5 MPa	1460:1488	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	2	38	theme	mechanical	436:445	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	4	39	theme	structures	735:744	arg1	behavior					700:707	the brittle behavior	688:707	the brittle behavior of most 3D chitosan-based structures	688:744	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	40	theme	micro-CT	945:952	arg1	analyzes					954:961	micro-CT analyzes	945:961	micro-CT analyzes	945:961	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	6	41	dep	obtained	1237:1244	arg1	hardness					1324:1331	indentation hardness	1312:1331	indentation hardness of 19.4 kgf/mm2	1312:1347	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	41	dep	obtained	1237:1244	arg1	strength					1287:1294	flexural strength	1278:1294	flexural strength of 53.8 MPa	1278:1306	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	4	42	theme	produced	838:845	arg1	specimens					847:855	the produced specimens	834:855	the produced specimens	834:855	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	43	theme	brittle	692:698	arg1	behavior					700:707	the brittle behavior	688:707	the brittle behavior of most 3D chitosan-based structures	688:744	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	2	44	theme	limited	428:434	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	45	theme	tissue	487:492	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	45	theme	tissue	487:492	arg1	strength					461:468	strength	461:468	strength	461:468	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	45	theme	tissue	487:492	arg1	reactions					494:502	the adverse tissue reactions	475:502	the adverse tissue reactions generated	475:512	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	1	46	theme	implant	313:319	arg1	removal					321:327	implant removal	313:327	implant removal	313:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	0	47	theme	mechanical	24:33	arg1	properties					35:44	the mechanical properties	20:44	the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications	20:126	Strategy to improve the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications.
32090961	5	48	theme	hydroxyapatite	1099:1112	arg1	ceramics					1035:1042	ceramics	1035:1042	ceramics	1035:1042	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	48	theme	hydroxyapatite	1099:1112	arg1	powder					1067:1072	hydroxyapatite powder	1052:1072	hydroxyapatite powder	1052:1072	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	48	theme	hydroxyapatite	1099:1112	arg1	mixtures					1087:1094	biphasic mixtures	1078:1094	biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP)	1078:1155	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	7	49	theme	implant	1628:1634	arg1	performance					1636:1646	a good implant performance	1621:1646	a good implant performance	1621:1646	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	2	50	theme	adverse	479:485	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	50	theme	adverse	479:485	arg1	strength					461:468	strength	461:468	strength	461:468	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	50	theme	adverse	479:485	arg1	reactions					494:502	the adverse tissue reactions	475:502	the adverse tissue reactions generated	475:512	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	7	51	theme	fundamental	1595:1605	arg1	condition					1607:1615	dense and homogeneous, fundamental condition	1572:1615	condition	1607:1615	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	7	51	theme	fundamental	1595:1605	arg1	compositions					1554:1565	All the tested material compositions	1530:1565	All the tested material compositions	1530:1565	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	4	52	theme	electron	915:922	arg1	microscopy					924:933	scanning electron microscopy	906:933	scanning electron microscopy (SEM)	906:939	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	52	theme	electron	915:922	arg1	SEM					936:938	SEM	936:938	SEM	936:938	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	0	53	theme	materials	63:71	arg1	properties					35:44	the mechanical properties	20:44	the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications	20:126	Strategy to improve the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications.
32090961	8	54	theme	orthopedic	1752:1761	arg1	applications					1772:1783	orthopedic fixation applications	1752:1783	orthopedic fixation applications	1752:1783	These are encouraging results, which support the continued development of chitosan-based materials for orthopedic fixation applications.
32090961	6	55	theme	best	1383:1386	arg1	behavior					1399:1406	the best mechanical behavior	1379:1406	the best mechanical behavior	1379:1406	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	56	theme	mechanical	1388:1397	arg1	behavior					1399:1406	the best mechanical behavior	1379:1406	the best mechanical behavior	1379:1406	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	4	57	theme	scanning	906:913	arg1	microscopy					924:933	scanning electron microscopy	906:933	scanning electron microscopy (SEM)	906:939	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	4	57	theme	scanning	906:913	arg1	SEM					936:938	SEM	936:938	SEM	936:938	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	5	58	theme	hydroxyapatite	1052:1065	arg1	ceramics					1035:1042	ceramics	1035:1042	ceramics	1035:1042	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	58	theme	hydroxyapatite	1052:1065	arg1	powder					1067:1072	hydroxyapatite powder	1052:1072	hydroxyapatite powder	1052:1072	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	6	59	theme	properties	1222:1231	arg1	combination					1194:1204	the best combination	1185:1204	the best combination of the measured properties	1185:1231	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	60	dep	powder	1452:1457	arg1	kgf/mm2					1520:1526	indentation hardness 28.2 kgf/mm2	1494:1526	indentation hardness 28.2 kgf/mm2	1494:1526	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	60	dep	powder	1452:1457	arg1	strength					1469:1476	flexural strength	1460:1476	flexural strength of 67.5 MPa	1460:1488	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	1	61	theme	Bioabsorbable	129:141	arg1	alternative					191:201	an alternative	188:201	an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal	188:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	61	theme	Bioabsorbable	129:141	arg1	devices					162:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	4	62	theme	plasticizers	818:829	arg1	effect					802:807	the effect	798:807	the effect of these plasticizers in the produced specimens	798:855	To overcome the brittle behavior of most 3D chitosan-based structures, glycerol and sorbitol were blended to chitosan and the effect of these plasticizers in the produced specimens was analyzed by flexural tests, Berkovich tests, scanning electron microscopy (SEM) and micro-CT analyzes.
32090961	6	63	theme	53.8 MPa	1299:1306	arg1	hardness					1324:1331	indentation hardness	1312:1331	indentation hardness of 19.4 kgf/mm2	1312:1347	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	63	theme	53.8 MPa	1299:1306	arg1	strength					1287:1294	flexural strength	1278:1294	flexural strength of 53.8 MPa	1278:1306	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	6	64	theme	measured	1213:1220	arg1	properties					1222:1231	the measured properties	1209:1231	the measured properties	1209:1231	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	1	65	from	implants	215:222	arg1	orthopedics					227:237	orthopedics	227:237	orthopedics	227:237	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	66	theme	polymeric	143:151	arg1	alternative					191:201	an alternative	188:201	an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal	188:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	66	theme	polymeric	143:151	arg1	devices					162:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	7	67	theme	dense	1572:1576	arg1	condition					1607:1615	dense and homogeneous, fundamental condition	1572:1615	condition	1607:1615	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	7	67	theme	dense	1572:1576	arg1	compositions					1554:1565	All the tested material compositions	1530:1565	All the tested material compositions	1530:1565	All the tested material compositions were dense and homogeneous, fundamental condition for a good implant performance.
32090961	1	68	dep	orthopedics	227:237	arg1	preventing					240:249	preventing	240:249	preventing the stress shielding effect	240:277	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	68	dep	orthopedics	227:237	arg1	avoiding					283:290	avoiding	283:290	avoiding a second surgery for implant removal	283:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	69	theme	fixation	153:160	arg1	alternative					191:201	an alternative	188:201	an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal	188:327	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	1	69	theme	fixation	153:160	arg1	devices					162:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices	129:168	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
32090961	6	70	theme	flexural	1460:1467	arg1	strength					1469:1476	flexural strength	1460:1476	flexural strength of 67.5 MPa	1460:1488	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	2	71	theme	several	339:345	arg1	problems					347:354	several problems	339:354	several problems	339:354	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	5	72	theme	biphasic	1078:1085	arg1	ceramics					1035:1042	ceramics	1035:1042	ceramics	1035:1042	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	72	theme	biphasic	1078:1085	arg1	mixtures					1087:1094	biphasic mixtures	1078:1094	biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP)	1078:1155	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	3	73	theme	new	635:637	arg1	materials					647:655	new implant materials	635:655	new implant materials based on chitosan	635:673	To minimize or even eliminate the problems associated with these implants, strategies have been developed to synthesize new implant materials based on chitosan.
32090961	2	74	theme	current	382:388	arg1	implants					404:411	current bioabsorbable implants	382:411	current bioabsorbable implants	382:411	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	74	theme	current	382:388	arg1	strength					461:468	strength	461:468	strength	461:468	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	2	74	theme	current	382:388	arg1	stiffness					447:455	the limited mechanical stiffness	424:455	the limited mechanical stiffness	424:455	However, several problems are still associated with current bioabsorbable implants, including the limited mechanical stiffness and strength, and the adverse tissue reactions generated.
32090961	6	75	theme	indentation	1312:1322	arg1	hardness					1324:1331	indentation hardness	1312:1331	indentation hardness of 19.4 kgf/mm2	1312:1347	In the plasticizers group, the best combination of the measured properties was obtained for chitosan with 10% glycerol (flexural strength of 53.8 MPa and indentation hardness of 19.4 kgf/mm2), while in the ceramics group the best mechanical behavior was obtained for chitosan with 10% HA+β-TCP powder (flexural strength of 67.5 MPa and indentation hardness 28.2 kgf/mm2).
32090961	0	76	theme	bioabsorbable	49:61	arg1	materials					63:71	bioabsorbable materials	49:71	bioabsorbable materials based on chitosan for orthopedic fixation applications	49:126	Strategy to improve the mechanical properties of bioabsorbable materials based on chitosan for orthopedic fixation applications.
32090961	5	77	theme	phosphate	1139:1147	arg1	ceramics					1035:1042	ceramics	1035:1042	ceramics	1035:1042	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	77	theme	phosphate	1139:1147	arg1	powder					1067:1072	hydroxyapatite powder	1052:1072	hydroxyapatite powder	1052:1072	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	5	77	theme	phosphate	1139:1147	arg1	mixtures					1087:1094	biphasic mixtures	1078:1094	biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP)	1078:1155	The improvement of the mechanical properties was also tested by adding ceramics, namely hydroxyapatite powder and biphasic mixtures of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
32090961	3	78	theme	implant	639:645	arg1	materials					647:655	new implant materials	635:655	new implant materials based on chitosan	635:673	To minimize or even eliminate the problems associated with these implants, strategies have been developed to synthesize new implant materials based on chitosan.
32090961	1	79	theme	stress	255:260	arg1	effect					272:277	the stress shielding effect	251:277	the stress shielding effect	251:277	Bioabsorbable polymeric fixation devices have been used as an alternative to metallic implants in orthopedics, preventing the stress shielding effect and avoiding a second surgery for implant removal.
29477805	3	0	theme	Violet	976:981	arg1	staining					988:995	Crystal Violet (CV) staining	968:995	Crystal Violet (CV) staining	968:995	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	4	1	theme	increased	1289:1297	arg1	activity					1309:1316	increased autolysis activity	1289:1316	increased autolysis activity	1289:1316	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	5	2	theme	CV	1447:1448	arg1	staining					1450:1457	CV staining	1447:1457	CV staining	1447:1457	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	1	3	theme	processing	251:260	arg1	environments					262:273	food processing environments	246:273	food processing environments	246:273	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	2	4	theme	surface	632:638	arg1	proteins					640:647	27 predicted surface proteins	619:647	27 predicted surface proteins	619:647	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	5	theme	ΔsrtA	1025:1029	arg1	mutant					1031:1036	the ΔsrtA mutant	1021:1036	the ΔsrtA mutant	1021:1036	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	6	theme	capsular	731:738	arg1	CPS1-4					757:762	CPS1-4	757:762	CPS1-4	757:762	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	6	theme	capsular	731:738	arg1	polysaccharides					740:754	capsular polysaccharides	731:754	capsular polysaccharides (CPS1-4, Δcps1-4)	731:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	7	from	deficient	700:708	arg1	production					717:726	the production	713:726	the production of capsular polysaccharides (CPS1-4, Δcps1-4)	713:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	5	8	theme	biofilm	1417:1423	arg1	formation					1425:1433	reduced biofilm formation	1409:1433	reduced biofilm formation measured by CV staining	1409:1457	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	3	9	attach	attachment	900:909	arg1	polystyrene					923:933	polystyrene	923:933	polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain	923:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	3	9	attach	attachment	900:909	arg2	cells					914:918	cells	914:918	cells	914:918	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	1	10	theme	spoilage	192:199	arg1	organism					201:208	a food spoilage organism	185:208	a food spoilage organism	185:208	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	1	10	theme	spoilage	192:199	arg1	plantarum					158:166	Lactobacillus plantarum	144:166	Lactobacillus plantarum	144:166	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	3	11	theme	imposed	843:849	arg1	modifications					864:876	the imposed cell surface modifications	839:876	the imposed cell surface modifications	839:876	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	4	12	theme	higher	1202:1207	arg1	presence					1209:1216	a higher presence	1200:1216	a higher presence of extracellular DNA (eDNA) in cps1 mutants	1200:1260	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	2	13	theme	Sortase	570:576	arg1	A					578:578	Sortase A	570:578	Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	570:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	4	14	theme	samples	1181:1187	arg1	analysis					1161:1168	Fluorescence microscopy analysis	1137:1168	Fluorescence microscopy analysis of biofilm samples	1137:1187	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	2	15	theme	surface	500:506	arg1	composition					508:518	cell surface composition	495:518	cell surface composition	495:518	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	16	theme	Surface	775:781	arg1	adhesion					783:790	Surface adhesion	775:790	Surface adhesion	775:790	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	0	17	theme	static	46:51	arg1	formation					61:69	static biofilm formation	46:69	static biofilm formation by Lactobacillus plantarum WCFS1	46:102	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	3	18	dep	increased	1056:1064	arg1	compared					1103:1110	compared	1103:1110	compared to the wild-type strain	1103:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	4	19	theme	microscopy	1150:1159	arg1	analysis					1161:1168	Fluorescence microscopy analysis	1137:1168	Fluorescence microscopy analysis of biofilm samples	1137:1187	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	1	20	theme	products	339:346	arg1	contamination					298:310	contamination	298:310	contamination	298:310	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	1	20	theme	products	339:346	arg1	recontamination					315:329	recontamination	315:329	recontamination	315:329	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	3	21	theme	surface	856:862	arg1	modifications					864:876	the imposed cell surface modifications	839:876	the imposed cell surface modifications	839:876	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	0	22	from	Role	0:3	arg1	formation					61:69	static biofilm formation	46:69	static biofilm formation by Lactobacillus plantarum WCFS1	46:102	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	3	23	theme	formation	804:812	arg1	studies					814:820	Surface adhesion and biofilm formation studies	775:820	Surface adhesion and biofilm formation studies	775:820	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	0	24	theme	Lactobacillus	74:86	arg1	WCFS1					98:102	Lactobacillus plantarum WCFS1	74:102	Lactobacillus plantarum WCFS1	74:102	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	4	25	theme	DNA	1235:1237	arg1	presence					1209:1216	a higher presence	1200:1216	a higher presence of extracellular DNA (eDNA) in cps1 mutants	1200:1260	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	5	26	theme	Subsequent	1319:1328	arg1	studies					1330:1336	Subsequent studies	1319:1336	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour	1319:1398	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	1	27	from	applications	113:124	arg1	fermentations					129:141	fermentations	129:141	fermentations	129:141	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	2	28	theme	comparative	437:447	arg1	analysis					449:456	comparative analysis	437:456	comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4)	437:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	5	29	theme	matrix	1526:1531	arg1	build-up					1502:1509	the build-up	1498:1509	the build-up of the biofilm matrix with eDNA	1498:1541	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	2	30	theme	wall	661:664	arg1	peptidoglycan					666:678	the cell wall peptidoglycan	652:678	the cell wall peptidoglycan (ΔsrtA)	652:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	30	theme	wall	661:664	arg1	ΔsrtA					681:685	ΔsrtA	681:685	ΔsrtA	681:685	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	0	31	theme	cell	8:11	arg1	surface					13:19	cell surface composition and lysis	8:41	surface	13:19	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	3	32	theme	cells	914:918	arg1	attachment					900:909	the initial attachment	888:909	the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain	888:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	33	theme	mutants	475:481	arg1	analysis					449:456	comparative analysis	437:456	comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4)	437:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	34	theme	L.	410:411	arg1	WCFS1					423:427	L. plantarum WCFS1	410:427	L. plantarum WCFS1	410:427	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	35	theme	type	466:469	arg1	analysis					449:456	comparative analysis	437:456	comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4)	437:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	0	36	theme	lysis	37:41	arg1	Role					0:3	Role	0:3	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.	0:103	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	3	37	theme	biofilm	941:947	arg1	formation					949:957	biofilm formation	941:957	biofilm formation based on Crystal Violet (CV) staining	941:995	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	38	from	deficient	539:547	arg1	production					556:565	the production	552:565	the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	552:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	39	theme	new	369:371	arg1	insights					373:380	new insights	369:380	new insights into biofilm development	369:405	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	40	theme	Crystal	968:974	arg1	Violet					976:981	Crystal Violet	968:981	Crystal Violet (CV) staining	968:995	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	3	40	theme	Crystal	968:974	arg1	CV					984:985	CV	984:985	CV	984:985	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	41	attach	attachment	605:614	arg1	peptidoglycan					666:678	the cell wall peptidoglycan	652:678	the cell wall peptidoglycan (ΔsrtA)	652:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	41	attach	attachment	605:614	arg1	ΔsrtA					681:685	ΔsrtA	681:685	ΔsrtA	681:685	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	41	attach	attachment	605:614	arg2	proteins					640:647	27 predicted surface proteins	619:647	27 predicted surface proteins	619:647	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	42	theme	covalent	596:603	arg1	attachment					605:614	the covalent attachment	592:614	the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	592:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	1	43	theme	food	246:249	arg1	environments					262:273	food processing environments	246:273	food processing environments	246:273	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	2	44	theme	predicted	622:630	arg1	proteins					640:647	27 predicted surface proteins	619:647	27 predicted surface proteins	619:647	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	45	theme	polysaccharides	740:754	arg1	production					717:726	the production	713:726	the production of capsular polysaccharides (CPS1-4, Δcps1-4)	713:772	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	4	46	theme	autolysis	1299:1307	arg1	activity					1309:1316	increased autolysis activity	1289:1316	increased autolysis activity	1289:1316	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	5	47	theme	reduced	1409:1415	arg1	formation					1425:1433	reduced biofilm formation	1409:1433	reduced biofilm formation measured by CV staining	1409:1457	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	5	48	with	build-up	1502:1509	arg1	eDNA					1538:1541	eDNA	1538:1541	eDNA	1538:1541	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	4	49	theme	extracellular	1221:1233	arg1	eDNA					1240:1243	eDNA	1240:1243	eDNA	1240:1243	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	4	49	theme	extracellular	1221:1233	arg1	DNA					1235:1237	extracellular DNA	1221:1237	extracellular DNA (eDNA)	1221:1244	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	1	50	theme	food	187:190	arg1	spoilage					192:199	a food spoilage	185:199	a food spoilage organism	185:208	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	1	51	from	biofilms	234:241	arg1	dispersal					219:227	its dispersal	215:227	its dispersal from biofilms in food processing environments	215:273	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	1	51	from	biofilms	234:241	arg1	environments					262:273	food processing environments	246:273	food processing environments	246:273	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	2	52	theme	deficient	539:547	arg1	mutants					531:537	mutants	531:537	mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	531:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	53	theme	deficient	700:708	arg1	mutants					531:537	mutants	531:537	mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	531:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	54	from	production	717:726	arg1	deficient					700:708	deficient	700:708	deficient	700:708	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	4	55	attach	presence	1209:1216	arg2	eDNA					1240:1243	eDNA	1240:1243	eDNA	1240:1243	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	4	55	attach	presence	1209:1216	arg2	DNA					1235:1237	extracellular DNA	1221:1237	extracellular DNA (eDNA)	1221:1244	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	4	55	attach	presence	1209:1216	arg1	mutants					1254:1260	cps1 mutants	1249:1260	cps1 mutants	1249:1260	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	2	56	theme	A	578:578	arg1	production					556:565	the production	552:565	the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	552:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	57	theme	cell	851:854	arg1	modifications					864:876	the imposed cell surface modifications	839:876	the imposed cell surface modifications	839:876	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	4	58	theme	Fluorescence	1137:1148	arg1	microscopy					1150:1159	Fluorescence microscopy	1137:1159	Fluorescence microscopy analysis of biofilm samples	1137:1187	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	3	59	theme	biofilm	796:802	arg1	formation					804:812	biofilm formation	796:812	biofilm formation	796:812	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	0	60	theme	biofilm	53:59	arg1	formation					61:69	static biofilm formation	46:69	static biofilm formation by Lactobacillus plantarum WCFS1	46:102	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	2	61	theme	cell	495:498	arg1	composition					508:518	cell surface composition	495:518	cell surface composition	495:518	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	62	theme	adhesion	783:790	arg1	studies					814:820	Surface adhesion and biofilm formation studies	775:820	Surface adhesion and biofilm formation studies	775:820	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	1	63	theme	food	334:337	arg1	products					339:346	food products	334:346	food products	334:346	Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products.
29477805	5	64	theme	lysis	1488:1492	arg1	relevance					1475:1483	the relevance	1471:1483	the relevance of lysis for the build-up of the biofilm matrix with eDNA	1471:1541	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	2	65	theme	cell	656:659	arg1	peptidoglycan					666:678	the cell wall peptidoglycan	652:678	the cell wall peptidoglycan (ΔsrtA)	652:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	65	theme	cell	656:659	arg1	ΔsrtA					681:685	ΔsrtA	681:685	ΔsrtA	681:685	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	0	66	theme	plantarum	88:96	arg1	WCFS1					98:102	Lactobacillus plantarum WCFS1	74:102	Lactobacillus plantarum WCFS1	74:102	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	3	67	theme	modifications	864:876	arg1	none					831:834	none	831:834	none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain	831:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	68	theme	biofilm	387:393	arg1	development					395:405	biofilm development	387:405	biofilm development	387:405	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	4	69	from	presence	1209:1216	arg1	mutants					1254:1260	cps1 mutants	1249:1260	cps1 mutants	1249:1260	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	3	70	theme	initial	892:898	arg1	attachment					900:909	the initial attachment	888:909	the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain	888:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	0	71	theme	surface	13:19	arg1	Role					0:3	Role	0:3	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.	0:103	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	2	72	theme	proteins	640:647	arg1	attachment					605:614	the covalent attachment	592:614	the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA)	592:686	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	2	73	theme	plantarum	413:421	arg1	WCFS1					423:427	L. plantarum WCFS1	410:427	L. plantarum WCFS1	410:427	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	3	74	theme	cps1	1089:1092	arg1	cluster					1094:1100	the cps1 cluster	1085:1100	the cps1 cluster	1085:1100	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	3	75	theme	wild-type	1119:1127	arg1	strain					1129:1134	the wild-type strain	1115:1134	the wild-type strain	1115:1134	Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain.
29477805	2	76	theme	wild	461:464	arg1	type					466:469	wild type	461:469	wild type	461:469	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	5	77	theme	lytic	1384:1388	arg1	behaviour					1390:1398	lytic behaviour	1384:1398	lytic behaviour	1384:1398	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	0	78	dep	surface	13:19	arg1	composition					21:31	composition	21:31	composition	21:31	Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1.
29477805	2	79	dep	CPS1-4	757:762	arg1	Δcps1-4					765:771	Δcps1-4	765:771	Δcps1-4	765:771	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	5	80	theme	biofilm	1518:1524	arg1	matrix					1526:1531	the biofilm matrix	1514:1531	the biofilm matrix	1514:1531	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	4	81	theme	cps1	1249:1252	arg1	mutants					1254:1260	cps1 mutants	1249:1260	cps1 mutants	1249:1260	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	4	82	theme	biofilm	1173:1179	arg1	samples					1181:1187	biofilm samples	1173:1187	biofilm samples	1173:1187	Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity.
29477805	5	83	theme	Δacm2	1344:1348	arg1	derivatives					1360:1370	Δacm2 and ΔlytA derivatives	1344:1370	Δacm2 and ΔlytA derivatives affected in lytic behaviour	1344:1398	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29477805	2	84	from	production	556:565	arg1	deficient					539:547	deficient	539:547	deficient	539:547	This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1-4, Δcps1-4).
29477805	5	85	theme	ΔlytA	1354:1358	arg1	derivatives					1360:1370	Δacm2 and ΔlytA derivatives	1344:1370	Δacm2 and ΔlytA derivatives affected in lytic behaviour	1344:1398	Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
29753767	5	0	from	%	917:917	arg1	agreement					929:937	close agreement	923:937	close agreement with the predicted theoretical value	923:974	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	2	1	dep	yield	251:255	arg1	the					247:249	the	247:249	the	247:249	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	5	2	theme	theoretical	958:968	arg1	value					970:974	the predicted theoretical value	944:974	the predicted theoretical value	944:974	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	5	3	theme	theoretical	811:821	arg1	value					823:827	the predicted theoretical value	797:827	the predicted theoretical value despite the good fit of the model (R2 = 0.96)	797:873	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	2	4	theme	ponkan	300:305	arg1	peel					307:310	ponkan peel	300:310	ponkan peel	300:310	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	7	5	theme	acetylation	1177:1187	arg1	degree					1167:1172	a very low degree	1156:1172	a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol	1156:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	7	6	theme	average	1200:1206	arg1	mass					1214:1217	average molar mass	1200:1217	average molar mass of 80,650 g/mol	1200:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	6	7	theme	I	1095:1095	arg1	region					1097:1102	a rhamnogalacturonan I region	1074:1102	a rhamnogalacturonan I region mainly branched by galactans	1074:1131	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	3	8	theme	uronic	474:479	arg1	content					486:492	the uronic acid content	470:492	the uronic acid content	470:492	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	4	9	theme	liquid	674:679	arg1	liquid					674:679	liquid	674:679	liquid	674:679	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	9	theme	liquid	674:679	arg1	100 min					662:668	100 min	662:668	100 min	662:668	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	9	theme	liquid	674:679	arg1	pH 1.6					635:640	pH 1.6	635:640	pH 1.6	635:640	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	9	theme	liquid	674:679	arg1	time					654:657	extraction time	643:657	extraction time	643:657	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	9	theme	liquid	674:679	arg1	conditions					541:550	The conditions	537:550	The conditions that resulted in the highest yield and highest uronic acid content	537:617	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	2	10	theme	experimental	143:154	arg1	design					156:161	A central composite experimental design	123:161	A central composite experimental design	123:161	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	4	11	theme	solid	681:685	arg1	ratio					687:691	solid ratio	681:691	solid ratio of 36 mL/g	681:702	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	3	12	theme	acid	481:484	arg1	content					486:492	the uronic acid content	470:492	the uronic acid content	470:492	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	5	13	theme	experimental	762:773	arg1	yield					775:779	an experimental yield	759:779	an experimental yield of 25.6%	759:788	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	2	14	used	used	167:170	arg2	design					156:161	A central composite experimental design	123:161	A central composite experimental design	123:161	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	15	theme	composite	133:141	arg1	design					156:161	A central composite experimental design	123:161	A central composite experimental design	123:161	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	5	16	theme	good	841:844	arg1	fit					846:848	the good fit	837:848	the good fit of the model (R2 = 0.96)	837:873	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	7	17	theme	low	1163:1165	arg1	degree					1167:1172	a very low degree	1156:1172	a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol	1156:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	4	18	theme	36 mL/g	696:702	arg1	ratio					687:691	solid ratio	681:691	solid ratio of 36 mL/g	681:702	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	2	19	dep	liquid	225:230	arg1	solid					232:236	solid	232:236	solid	232:236	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	20	from	influence	188:196	arg1	yield					251:255	yield	251:255	yield	251:255	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	20	from	influence	188:196	arg1	content					273:279	uronic acid content	261:279	uronic acid content	261:279	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	21	theme	central	125:131	arg1	design					156:161	A central composite experimental design	123:161	A central composite experimental design	123:161	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	22	from	peel	307:310	arg1	yield					251:255	yield	251:255	yield	251:255	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	22	from	peel	307:310	arg1	content					273:279	uronic acid content	261:279	uronic acid content	261:279	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	23	theme	ratio	238:242	arg1	influence					188:196	the influence	184:196	the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel	184:310	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	3	24	theme	lower	396:400	arg1	pHs					402:404	lower pHs	396:404	lower pHs	396:404	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	5	25	contain	had	755:757	arg1	pectin					709:714	The pectin	705:714	The pectin obtained under these conditions (PPOP)	705:753	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	5	25	contain	had	755:757	arg2	yield					775:779	an experimental yield	759:779	an experimental yield of 25.6%	759:788	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	8	26	theme	citrus	1331:1336	arg1	pectin					1338:1343	citrus pectin	1331:1343	citrus pectin	1331:1343	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	6	27	theme	%	1068:1068	arg1	esterification					1046:1059	methyl esterification	1039:1059	methyl esterification of 85.7%	1039:1068	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	4	28	theme	uronic	599:604	arg1	content					611:617	highest uronic acid content	591:617	highest uronic acid content	591:617	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	2	29	theme	acid	268:271	arg1	content					273:279	uronic acid content	261:279	uronic acid content	261:279	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	4	30	theme	acid	606:609	arg1	content					611:617	highest uronic acid content	591:617	highest uronic acid content	591:617	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	3	31	theme	response	317:324	arg1	methodology					334:344	The response surface methodology	313:344	The response surface methodology	313:344	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	4	32	theme	100 min	662:668	arg1	liquid					674:679	liquid	674:679	liquid	674:679	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	32	theme	100 min	662:668	arg1	100 min					662:668	100 min	662:668	100 min	662:668	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	32	theme	100 min	662:668	arg1	pH 1.6					635:640	pH 1.6	635:640	pH 1.6	635:640	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	32	theme	100 min	662:668	arg1	time					654:657	extraction time	643:657	extraction time	643:657	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	32	theme	100 min	662:668	arg1	conditions					541:550	The conditions	537:550	The conditions that resulted in the highest yield and highest uronic acid content	537:617	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	6	33	theme	rhamnogalacturonan	1076:1093	arg1	region					1097:1102	a rhamnogalacturonan I region	1074:1102	a rhamnogalacturonan I region mainly branched by galactans	1074:1131	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	2	34	theme	uronic	261:266	arg1	content					273:279	uronic acid content	261:279	uronic acid content	261:279	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	5	35	theme	acid	896:899	arg1	content					901:907	the galacturonic acid content	879:907	the galacturonic acid content	879:907	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	5	35	theme	acid	896:899	arg1	%					917:917	84.5%	913:917	84.5%	913:917	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	3	36	theme	longer	407:412	arg1	times					425:429	longer extraction times	407:429	longer extraction times	407:429	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	0	37	theme	pectin	14:19	arg1	Extraction					0:9	Extraction	0:9	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.	0:61	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	4	38	dep	defined	624:630	arg1	ratio					687:691	solid ratio	681:691	solid ratio of 36 mL/g	681:702	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	3	39	theme	liquid	442:447	arg1	ratio					455:459	higher liquid:solid ratio	435:459	higher liquid:solid ratio	435:459	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	5	40	with	agreement	929:937	arg1	value					970:974	the predicted theoretical value	944:974	the predicted theoretical value	944:974	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	3	41	theme	extraction	414:423	arg1	times					425:429	longer extraction times	407:429	longer extraction times	407:429	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	2	42	theme	pectin	288:293	arg1	yield					251:255	yield	251:255	yield	251:255	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	2	42	theme	pectin	288:293	arg1	content					273:279	uronic acid content	261:279	uronic acid content	261:279	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	7	43	contain	had	1152:1154	arg2	degree					1167:1172	a very low degree	1156:1172	a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol	1156:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	7	43	contain	had	1152:1154	arg1	PPOP					1147:1150	PPOP	1147:1150	PPOP	1147:1150	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	2	44	theme	time	216:219	arg1	influence					188:196	the influence	184:196	the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel	184:310	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	0	45	theme	ponkan	26:31	arg1	cv					59:60	ponkan (Citrus reticulata Blanco cv	26:60	ponkan (Citrus reticulata Blanco cv	26:60	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	6	46	with	homogalacturonan	1007:1022	arg1	degree					1029:1034	degree	1029:1034	degree of methyl esterification of 85.7%	1029:1068	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	0	47	from	cv	59:60	arg1	Extraction					0:9	Extraction	0:9	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.	0:61	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	2	48	theme	extraction	205:214	arg1	time					216:219	extraction time	205:219	extraction time	205:219	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	7	49	theme	molar	1208:1212	arg1	mass					1214:1217	average molar mass	1200:1217	average molar mass of 80,650 g/mol	1200:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	0	50	theme	reticulata	41:50	arg1	cv					59:60	ponkan (Citrus reticulata Blanco cv	26:60	ponkan (Citrus reticulata Blanco cv	26:60	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	8	51	used	used	1311:1314	arg2	peel					1299:1302	ponkan peel	1292:1302	ponkan peel	1292:1302	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	8	51	used	used	1311:1314	arg2	source					1321:1326	a source	1319:1326	a source of citrus pectin in the regions where this species is cultivated	1319:1391	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	5	52	theme	predicted	948:956	arg1	value					970:974	the predicted theoretical value	944:974	the predicted theoretical value	944:974	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	3	53	theme	extraction	520:529	arg1	time					531:534	extraction time	520:534	extraction time	520:534	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	8	54	from	source	1321:1326	arg1	regions					1352:1358	the regions	1348:1358	the regions where this species is cultivated	1348:1391	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	4	55	theme	extraction	643:652	arg1	liquid					674:679	liquid	674:679	liquid	674:679	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	55	theme	extraction	643:652	arg1	100 min					662:668	100 min	662:668	100 min	662:668	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	55	theme	extraction	643:652	arg1	pH 1.6					635:640	pH 1.6	635:640	pH 1.6	635:640	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	4	55	theme	extraction	643:652	arg1	time					654:657	extraction time	643:657	extraction time	643:657	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	0	56	theme	Citrus	34:39	arg1	cv					59:60	ponkan (Citrus reticulata Blanco cv	26:60	ponkan (Citrus reticulata Blanco cv	26:60	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	6	57	theme	esterification	1046:1059	arg1	degree					1029:1034	degree	1029:1034	degree of methyl esterification of 85.7%	1029:1068	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	8	58	theme	pectin	1338:1343	arg1	source					1321:1326	a source	1319:1326	a source of citrus pectin in the regions where this species is cultivated	1319:1391	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	8	58	theme	pectin	1338:1343	arg1	peel					1299:1302	ponkan peel	1292:1302	ponkan peel	1292:1302	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	2	59	theme	pH	201:202	arg1	influence					188:196	the influence	184:196	the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel	184:310	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	1	60	theme	structural	94:103	arg1	characterization					105:120	structural characterization	94:120	structural characterization	94:120	Ponkan) peel: Optimization and structural characterization.
29753767	5	61	theme	galacturonic	883:894	arg1	content					901:907	the galacturonic acid content	879:907	the galacturonic acid content	879:907	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	5	61	theme	galacturonic	883:894	arg1	%					917:917	84.5%	913:917	84.5%	913:917	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	6	62	theme	methyl	1039:1044	arg1	esterification					1046:1059	methyl esterification	1039:1059	methyl esterification of 85.7%	1039:1068	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29753767	7	63	theme	light	1250:1254	arg1	scattering					1256:1265	light scattering	1250:1265	light scattering	1250:1265	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	7	64	theme	80,650 g/mol	1222:1233	arg1	mass					1214:1217	average molar mass	1200:1217	average molar mass of 80,650 g/mol	1200:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	7	64	theme	80,650 g/mol	1222:1233	arg1	acetylation					1177:1187	acetylation	1177:1187	acetylation (0.1%)	1177:1194	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	7	64	theme	80,650 g/mol	1222:1233	arg1	%					1193:1193	0.1%	1190:1193	0.1%	1190:1193	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	3	65	dep	liquid	442:447	arg1	higher					435:440	higher	435:440	higher	435:440	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	3	65	dep	liquid	442:447	arg1	solid					449:453	solid	449:453	solid	449:453	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	5	66	theme	predicted	801:809	arg1	value					823:827	the predicted theoretical value	797:827	the predicted theoretical value despite the good fit of the model (R2 = 0.96)	797:873	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	7	67	theme	mass	1214:1217	arg1	degree					1167:1172	a very low degree	1156:1172	a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol	1156:1233	In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering.
29753767	8	68	theme	ponkan	1292:1297	arg1	source					1321:1326	a source	1319:1326	a source of citrus pectin in the regions where this species is cultivated	1319:1391	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	8	68	theme	ponkan	1292:1297	arg1	peel					1299:1302	ponkan peel	1292:1302	ponkan peel	1292:1302	The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.
29753767	5	69	theme	close	923:927	arg1	agreement					929:937	close agreement	923:937	close agreement with the predicted theoretical value	923:974	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	2	70	theme	liquid	225:230	arg1	ratio					238:242	liquid:solid ratio	225:242	liquid:solid ratio	225:242	A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel.
29753767	4	71	theme	highest	573:579	arg1	yield					581:585	the highest yield	569:585	the highest yield	569:585	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	5	72	theme	%	788:788	arg1	yield					775:779	an experimental yield	759:779	an experimental yield of 25.6%	759:788	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	4	73	theme	highest	591:597	arg1	content					611:617	highest uronic acid content	591:617	highest uronic acid content	591:617	The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g.
29753767	0	74	theme	Blanco	52:57	arg1	cv					59:60	ponkan (Citrus reticulata Blanco cv	26:60	ponkan (Citrus reticulata Blanco cv	26:60	Extraction of pectin from ponkan (Citrus reticulata Blanco cv.
29753767	5	75	theme	model	857:861	arg1	fit					846:848	the good fit	837:848	the good fit of the model (R2 = 0.96)	837:873	The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value.
29753767	3	76	theme	surface	326:332	arg1	methodology					334:344	The response surface methodology	313:344	The response surface methodology	313:344	The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time.
29753767	6	77	with	region	1097:1102	arg1	degree					1029:1034	degree	1029:1034	degree of methyl esterification of 85.7%	1029:1068	PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans.
29561781	7	0	theme	evolutionary	1176:1187	arg1	context					1189:1195	their evolutionary context	1170:1195	their evolutionary context	1170:1195	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	7	1	theme	Chlamydomonas	1083:1095	arg1	reinhardtii					1097:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	5	2	theme	charophycean	683:694	arg1	algae					702:706	the charophycean green algae	679:706	the charophycean green algae (CGA)	679:712	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	2	theme	charophycean	683:694	arg1	ancestors					738:746	the immediate ancestors	724:746	the immediate ancestors of land plants	724:761	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	2	theme	charophycean	683:694	arg1	CGA					709:711	CGA	709:711	CGA	709:711	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	2	3	gly	N-glycosylated	292:305	arg1	proteins					247:254	Many proteins	242:254	Many proteins that are secreted by eukaryotes	242:286	Many proteins that are secreted by eukaryotes are N-glycosylated.
29561781	9	4	theme	other	1603:1607	arg1	processes					1623:1631	other extracellular processes	1603:1631	plant cell wall modification as well as other extracellular processes	1563:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	7	5	dep	thaliana	1147:1154	arg1	Arabidopsis					1135:1145	the model land plant, Arabidopsis thaliana	1113:1154	Arabidopsis	1135:1145	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	4	6	theme	green	525:529	arg1	algae					531:535	chlorophyte green algae	513:535	chlorophyte green algae	513:535	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	7	7	theme	green	1072:1076	arg1	reinhardtii					1097:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	9	8	theme	proteins	1538:1545	arg1	many					1526:1529	many	1526:1529	many	1526:1529	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	9	8	theme	proteins	1538:1545	arg1	proteins					1538:1545	the proteins	1534:1545	the proteins associated with plant cell wall modification as well as other extracellular processes	1534:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	7	9	theme	alga	1078:1081	arg1	reinhardtii					1097:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	7	10	theme	model	1117:1121	arg1	thaliana					1147:1154	the model land plant, Arabidopsis thaliana	1113:1154	thaliana	1147:1154	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	6	11	gly	N-glycoproteins	904:918	arg1	N-glycoproteins					904:918	secreted N-glycoproteins	895:918	secreted N-glycoproteins of Penium margaritaceum	895:942	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	6	12	theme	mass	837:840	arg1	spectrometry					842:853	mass spectrometry	837:853	mass spectrometry	837:853	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	5	13	theme	valuable	628:635	arg1	system					637:642	A potentially valuable system	614:642	A potentially valuable system to study this issue	614:662	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	0	14	theme	Secretome	4:12	arg1	Profiles					34:41	The Secretome and N-Glycosylation Profiles	0:41	Profiles	34:41	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	4	15	theme	land	497:500	arg1	plants					502:507	land plants	497:507	land plants	497:507	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	3	16	theme	N-glycosylation	385:399	arg1	patterns					401:408	N-glycosylation patterns	385:408	N-glycosylation patterns	385:408	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	0	17	theme	N-Glycosylation	18:32	arg1	Profiles					34:41	The Secretome and N-Glycosylation Profiles	0:41	Profiles	34:41	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	5	18	theme	green	696:700	arg1	algae					702:706	the charophycean green algae	679:706	the charophycean green algae (CGA)	679:712	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	18	theme	green	696:700	arg1	ancestors					738:746	the immediate ancestors	724:746	the immediate ancestors of land plants	724:761	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	18	theme	green	696:700	arg1	CGA					709:711	CGA	709:711	CGA	709:711	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	8	19	theme	margaritaceum	1369:1381	arg1	secretome					1383:1391	the P. margaritaceum secretome	1362:1391	the P. margaritaceum secretome	1362:1391	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	8	19	theme	margaritaceum	1369:1381	arg1	related					1409:1415	related	1409:1415	related	1409:1415	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	8	20	theme	P.	1366:1367	arg1	secretome					1383:1391	the P. margaritaceum secretome	1362:1391	the P. margaritaceum secretome	1362:1391	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	8	20	theme	P.	1366:1367	arg1	related					1409:1415	related	1409:1415	related	1409:1415	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	1	21	theme	proteins	179:186	arg1	population					165:174	the population	161:174	the population of proteins that are secreted into the extracellular environment	161:239	The secretome can be defined as the population of proteins that are secreted into the extracellular environment.
29561781	1	21	theme	proteins	179:186	arg1	secretome					133:141	The secretome	129:141	The secretome	129:141	The secretome can be defined as the population of proteins that are secreted into the extracellular environment.
29561781	3	22	theme	striking	327:334	arg1	differences					336:346	striking differences	327:346	striking differences in the diversity and conservation of N-glycosylation patterns between taxa	327:421	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	3	23	from	differences	336:346	arg1	conservation					369:380	conservation	369:380	conservation	369:380	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	3	23	from	differences	336:346	arg1	diversity					355:363	diversity	355:363	diversity	355:363	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	9	24	theme	study	1492:1496	arg1	results					1476:1482	The results	1472:1482	The results of this study	1472:1496	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	5	25	theme	land	751:754	arg1	plants					756:761	land plants	751:761	land plants	751:761	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	0	26	theme	Green	63:67	arg1	Alga					69:72	the Charophycean Green Alga	46:72	the Charophycean Green Alga	46:72	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	0	26	theme	Green	63:67	arg1	margaritaceum					82:94	Penium margaritaceum	75:94	Penium margaritaceum	75:94	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	9	27	theme	terrestrial	1670:1680	arg1	habitats					1682:1689	terrestrial habitats	1670:1689	terrestrial habitats	1670:1689	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	4	28	theme	secretome	441:449	arg1	structures					471:480	the secretome and N-glycosylation structures	437:480	structures	471:480	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	6	29	theme	margaritaceum	930:942	arg1	N-glycoproteins					904:918	secreted N-glycoproteins	895:918	secreted N-glycoproteins of Penium margaritaceum	895:942	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	8	30	theme	proteins	1251:1258	arg1	identification					1223:1236	the identification	1219:1236	the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes	1219:1469	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	5	31	theme	plants	756:761	arg1	algae					702:706	the charophycean green algae	679:706	the charophycean green algae (CGA)	679:712	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	31	theme	plants	756:761	arg1	ancestors					738:746	the immediate ancestors	724:746	the immediate ancestors of land plants	724:761	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	6	32	theme	Penium	923:928	arg1	margaritaceum					930:942	Penium margaritaceum	923:942	Penium margaritaceum	923:942	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	7	33	theme	land	1123:1126	arg1	thaliana					1147:1154	the model land plant, Arabidopsis thaliana	1113:1154	thaliana	1147:1154	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	8	34	theme	land	1429:1432	arg1	plants					1434:1439	land plants	1429:1439	land plants	1429:1439	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	7	35	theme	plant	1128:1132	arg1	thaliana					1147:1154	the model land plant, Arabidopsis thaliana	1113:1154	thaliana	1147:1154	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	8	36	theme	wall	1246:1249	arg1	proteins					1251:1258	cell wall proteins	1241:1258	cell wall proteins	1241:1258	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	3	37	theme	patterns	401:408	arg1	conservation					369:380	conservation	369:380	conservation	369:380	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	3	37	theme	patterns	401:408	arg1	diversity					355:363	diversity	355:363	diversity	355:363	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	4	38	theme	N-glycosylation	455:469	arg1	structures					471:480	the secretome and N-glycosylation structures	437:480	structures	471:480	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	9	39	theme	plant	1563:1567	arg1	modification					1579:1590	plant cell wall modification	1563:1590	plant cell wall modification as well as other extracellular processes	1563:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	7	40	theme	identified	979:988	arg1	proteins					999:1006	The identified secreted proteins	975:1006	The identified secreted proteins	975:1006	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	8	41	theme	proteins	1264:1271	arg1	identification					1223:1236	the identification	1219:1236	the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes	1219:1469	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	9	42	theme	habitats	1682:1689	arg1	colonization					1654:1665	the colonization	1650:1665	the colonization of terrestrial habitats	1650:1689	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	7	43	theme	chlorophyte	1060:1070	arg1	reinhardtii					1097:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	the chlorophyte green alga Chlamydomonas reinhardtii	1056:1107	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	9	44	theme	extracellular	1609:1621	arg1	processes					1623:1631	other extracellular processes	1603:1631	plant cell wall modification as well as other extracellular processes	1563:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	6	45	theme	secreted	895:902	arg1	N-glycoproteins					904:918	secreted N-glycoproteins	895:918	secreted N-glycoproteins of Penium margaritaceum	895:942	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	7	46	theme	secreted	990:997	arg1	proteins					999:1006	The identified secreted proteins	975:1006	The identified secreted proteins	975:1006	The identified secreted proteins and N-glycans were compared to those known from the chlorophyte green alga Chlamydomonas reinhardtii and the model land plant, Arabidopsis thaliana, to establish their evolutionary context.
29561781	6	47	theme	affinity	794:801	arg1	LAC					819:821	LAC	819:821	LAC	819:821	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	6	47	theme	affinity	794:801	arg1	chromatography					803:816	lectin affinity chromatography	787:816	lectin affinity chromatography (LAC) coupled with mass spectrometry	787:853	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	9	48	theme	wall	1574:1577	arg1	modification					1579:1590	plant cell wall modification	1563:1590	plant cell wall modification as well as other extracellular processes	1563:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	5	49	theme	immediate	728:736	arg1	algae					702:706	the charophycean green algae	679:706	the charophycean green algae (CGA)	679:712	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	5	49	theme	immediate	728:736	arg1	ancestors					738:746	the immediate ancestors	724:746	the immediate ancestors of land plants	724:761	A potentially valuable system to study this issue is provided by the charophycean green algae (CGA), which is the immediate ancestors of land plants.
29561781	3	50	dep	diversity	355:363	arg1	the					351:353	the	351:353	the	351:353	However, there are striking differences in the diversity and conservation of N-glycosylation patterns between taxa.
29561781	6	51	theme	lectin	787:792	arg1	LAC					819:821	LAC	819:821	LAC	819:821	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	6	51	theme	lectin	787:792	arg1	chromatography					803:816	lectin affinity chromatography	787:816	lectin affinity chromatography (LAC) coupled with mass spectrometry	787:853	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	4	52	theme	plant	597:601	arg1	evolution					603:611	plant evolution	597:611	plant evolution	597:611	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	0	53	theme	Alga	69:72	arg1	Profiles					34:41	The Secretome and N-Glycosylation Profiles	0:41	Profiles	34:41	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	2	54	theme	Many	242:245	arg1	proteins					247:254	Many proteins	242:254	Many proteins that are secreted by eukaryotes	242:286	Many proteins that are secreted by eukaryotes are N-glycosylated.
29561781	8	55	theme	cell	1241:1244	arg1	proteins					1251:1258	cell wall proteins	1241:1258	cell wall proteins	1241:1258	Our approach allowed the identification of cell wall proteins and proteins modified with N-glycans that are identical to those of embryophytes, which suggests that the P. margaritaceum secretome is more closely related to those of land plants than to those of chlorophytes.
29561781	1	56	theme	extracellular	215:227	arg1	environment					229:239	the extracellular environment	211:239	the extracellular environment	211:239	The secretome can be defined as the population of proteins that are secreted into the extracellular environment.
29561781	0	57	theme	Charophycean	50:61	arg1	Alga					69:72	the Charophycean Green Alga	46:72	the Charophycean Green Alga	46:72	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	0	57	theme	Charophycean	50:61	arg1	margaritaceum					82:94	Penium margaritaceum	75:94	Penium margaritaceum	75:94	The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.
29561781	4	58	theme	chlorophyte	513:523	arg1	algae					531:535	chlorophyte green algae	513:535	chlorophyte green algae	513:535	For example, the secretome and N-glycosylation structures differ between land plants and chlorophyte green algae, but it is not clear when this divergence took place during plant evolution.
29561781	6	59	used	used	782:785	arg2	we					779:780	we	779:780	we	779:780	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	9	60	theme	cell	1569:1572	arg1	modification					1579:1590	plant cell wall modification	1563:1590	plant cell wall modification as well as other extracellular processes	1563:1631	The results of this study support the hypothesis that many of the proteins associated with plant cell wall modification as well as other extracellular processes evolved prior to the colonization of terrestrial habitats.
29561781	6	61	theme	CGA	970:972	arg1	secretome					875:883	the secretome	871:883	the secretome	871:883	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
29561781	6	61	theme	CGA	970:972	arg1	member					956:961	a member	954:961	a member of the CGA	954:972	In this study, we used lectin affinity chromatography (LAC) coupled with mass spectrometry to characterize the secretome including secreted N-glycoproteins of Penium margaritaceum, which is a member of the CGA.
30698412	8	0	theme	virus	1444:1448	arg1	infection					1457:1465	Dengue virus (DENV) infection	1437:1465	Dengue virus (DENV) infection	1437:1465	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	3	1	theme	brain	624:628	arg1	cells					630:634	fetal brain cells	618:634	fetal brain cells	618:634	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	3	2	dep	mosquitoes	606:615	arg1	i.e.					600:603	i.e.	600:603	i.e.	600:603	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	11	3	theme	carbohydrate-based	1756:1773	arg1	antivirals					1775:1784	carbohydrate-based antivirals	1756:1784	carbohydrate-based antivirals against flaviviral infection	1756:1813	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	4	4	theme	albopictus	913:922	arg1	composition					874:884	the GAG composition	866:884	the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	866:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	7	5	theme	sulfate	1257:1263	arg1	amounts					1219:1225	the amounts	1215:1225	the amounts of hyaluronic acid or keratan sulfate	1215:1263	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	5	theme	sulfate	1257:1263	arg1	acid					1241:1244	hyaluronic acid	1230:1244	hyaluronic acid	1230:1244	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	5	theme	sulfate	1257:1263	arg1	sulfate					1257:1263	keratan sulfate	1249:1263	keratan sulfate	1249:1263	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	3	6	theme	eye	641:643	arg1	tissues					645:651	eye tissues	641:651	eye tissues	641:651	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	1	7	from	abnormalities	268:280	arg1	women					294:298	pregnant women	285:298	pregnant women	285:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	4	8	theme	unsulfated	797:806	arg1	0S					793:794	HS 0S	790:794	HS 0S (unsulfated HS)	790:810	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	8	theme	unsulfated	797:806	arg1	HS					808:809	unsulfated HS	797:809	unsulfated HS	797:809	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	7	9	theme	hyaluronic	1230:1239	arg1	acid					1241:1244	hyaluronic acid	1230:1244	hyaluronic acid	1230:1244	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	11	10	from	antivirals	1775:1784	arg1	pathogenesis					1739:1750	ZIKV pathogenesis	1734:1750	ZIKV pathogenesis	1734:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	3	11	theme	ZIKV	657:660	arg1	pathogenesis					662:673	ZIKV pathogenesis	657:673	ZIKV pathogenesis	657:673	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	9	12	theme	DENV	1518:1521	arg1	replication					1523:1533	DENV replication	1518:1533	DENV replication	1518:1533	All compounds effectively inhibited DENV replication; however, they surprisingly promoted ZIKV replication.
30698412	1	13	from	family	233:238	arg1	virus					207:211	an enveloped RNA virus	190:211	an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women	190:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	13	from	family	233:238	arg1	virus					174:178	Zika virus	169:178	Zika virus (ZIKV)	169:185	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	5	14	theme	human	939:943	arg1	progenitor					958:967	human fetal neural progenitor and differentiated cells	939:992	progenitor	958:967	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	3	15	theme	GAG	542:544	arg1	composition					559:569	the GAG disaccharide composition	538:569	the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis	538:673	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	1	16	theme	enveloped	193:201	arg1	virus					207:211	an enveloped RNA virus	190:211	an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women	190:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	16	theme	enveloped	193:201	arg1	virus					174:178	Zika virus	169:178	Zika virus (ZIKV)	169:185	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	17	theme	fetal	255:259	arg1	abnormalities					268:280	fetal neural abnormalities	255:280	fetal neural abnormalities in pregnant women	255:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	0	18	theme	Zika	147:150	arg1	Infection					158:166	Zika Virus Infection	147:166	Zika Virus Infection	147:166	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	10	19	theme	chain	1603:1607	arg1	lengths					1609:1615	longer chain lengths	1596:1615	longer chain lengths	1596:1615	HP of longer chain lengths more strongly promoted activity in ZIKV replication.
30698412	4	20	theme	0S	793:794	arg1	differences					851:861	the main differences	842:861	the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	842:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	20	theme	0S	793:794	arg1	levels					768:773	levels	768:773	levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides	768:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	6	21	theme	disaccharide	1052:1063	arg1	levels					1077:1082	disaccharide composition levels	1052:1082	disaccharide composition levels	1052:1082	A change in disaccharide composition levels was observed between undifferentiated and differentiated cells.
30698412	3	22	theme	other	574:578	arg1	tissues					591:597	other biological tissues	574:597	other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues)	574:652	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	2	23	theme	host	449:452	arg1	CS					427:428	CS	427:428	CS	427:428	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	23	theme	host	449:452	arg1	receptor					467:474	a potential host cell surface receptor	437:474	a potential host cell surface receptor	437:474	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	24	from	receptor	467:474	arg1	pathogenesis					484:495	ZIKV pathogenesis	479:495	ZIKV pathogenesis	479:495	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	11	25	from	role	1718:1721	arg1	pathogenesis					1739:1750	ZIKV pathogenesis	1734:1750	ZIKV pathogenesis	1734:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	3	26	theme	specific	709:716	arg1	GAGs					718:721	tissue specific GAGs	702:721	tissue specific GAGs	702:721	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	0	27	theme	Virus	69:73	arg1	Pathogenesis					75:86	Zika Virus Pathogenesis	64:86	Zika Virus Pathogenesis	64:86	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	4	28	theme	main	846:849	arg1	differences					851:861	the main differences	842:861	the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	842:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	28	theme	main	846:849	arg1	levels					768:773	levels	768:773	levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides	768:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	0	29	from	Analysis	32:39	arg1	Evaluation					101:110	in Vitro Evaluation	92:110	in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection	92:166	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	0	29	from	Analysis	32:39	arg1	Pathogenesis					75:86	Zika Virus Pathogenesis	64:86	Zika Virus Pathogenesis	64:86	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	5	30	theme	neural	951:956	arg1	progenitor					958:967	human fetal neural progenitor and differentiated cells	939:992	progenitor	958:967	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	2	31	theme	surface	459:465	arg1	CS					427:428	CS	427:428	CS	427:428	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	31	theme	surface	459:465	arg1	receptor					467:474	a potential host cell surface receptor	437:474	a potential host cell surface receptor	437:474	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	11	32	from	understanding	1701:1713	arg1	pathogenesis					1739:1750	ZIKV pathogenesis	1734:1750	ZIKV pathogenesis	1734:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	3	33	from	composition	559:569	arg1	pathogenesis					662:673	ZIKV pathogenesis	657:673	ZIKV pathogenesis	657:673	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	7	34	from	GAG	1206:1208	arg1	regions					1161:1167	different regions	1151:1167	different regions of the bovine eyes	1151:1186	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	0	35	theme	Glycosaminoglycan	0:16	arg1	Analysis					32:39	Glycosaminoglycan Compositional Analysis	0:39	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.	0:167	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	5	36	from	disaccharides	1025:1037	arg1	progenitor					958:967	human fetal neural progenitor and differentiated cells	939:992	progenitor	958:967	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	5	36	from	disaccharides	1025:1037	arg1	cells					988:992	human fetal neural progenitor and differentiated cells	939:992	cells	988:992	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	4	37	theme	aegypti	895:901	arg1	composition					874:884	the GAG composition	866:884	the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	866:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	8	38	theme	structures	1350:1359	arg1	heparin					1326:1332	heparin	1326:1332	heparin (HP) of various structures	1326:1359	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	8	38	theme	structures	1350:1359	arg1	HP					1335:1336	HP	1335:1336	HP	1335:1336	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	1	39	theme	neural	261:266	arg1	abnormalities					268:280	fetal neural abnormalities	255:280	fetal neural abnormalities in pregnant women	255:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	7	40	theme	bovine	1176:1181	arg1	eyes					1183:1186	the bovine eyes	1172:1186	the bovine eyes	1172:1186	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	2	41	theme	placental	375:383	arg1	CS					406:407	CS	406:407	CS	406:407	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	41	theme	placental	375:383	arg1	sulfate					397:403	human placental chondroitin sulfate	369:403	human placental chondroitin sulfate (CS)	369:408	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	0	42	theme	Relevant	44:51	arg1	Tissues					53:59	Relevant Tissues	44:59	Relevant Tissues	44:59	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	4	43	theme	GAG	870:872	arg1	composition					874:884	the GAG composition	866:884	the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	866:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	8	44	theme	potential	1382:1390	arg1	activity					1411:1418	their potential in vitro antiviral activity	1376:1418	their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells	1376:1479	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	3	45	theme	tissues	591:597	arg1	composition					559:569	the GAG disaccharide composition	538:569	the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis	538:673	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	7	46	theme	different	1151:1159	arg1	regions					1161:1167	different regions	1151:1167	different regions of the bovine eyes	1151:1186	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	3	47	theme	fetal	618:622	arg1	cells					630:634	fetal brain cells	618:634	fetal brain cells	618:634	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	1	48	theme	RNA	203:205	arg1	virus					207:211	an enveloped RNA virus	190:211	an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women	190:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	48	theme	RNA	203:205	arg1	virus					174:178	Zika virus	169:178	Zika virus (ZIKV)	169:185	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	11	49	theme	ZIKV	1734:1737	arg1	pathogenesis					1739:1750	ZIKV pathogenesis	1734:1750	ZIKV pathogenesis	1734:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	4	50	theme	Heparan	724:730	arg1	GAG					759:761	the major GAG	749:761	the major GAG	749:761	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	50	theme	Heparan	724:730	arg1	HS					741:742	HS	741:742	HS	741:742	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	50	theme	Heparan	724:730	arg1	sulfate					732:738	Heparan sulfate	724:738	Heparan sulfate (HS)	724:743	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	2	51	theme	envelope	342:349	arg1	ZIKV-EP					333:339	ZIKV-EP	333:339	ZIKV-EP (envelope protein)	333:358	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	51	theme	envelope	342:349	arg1	protein					351:357	envelope protein	342:357	envelope protein	342:357	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	9	52	theme	ZIKV	1572:1575	arg1	replication					1577:1587	ZIKV replication	1572:1587	ZIKV replication	1572:1587	All compounds effectively inhibited DENV replication; however, they surprisingly promoted ZIKV replication.
30698412	1	53	theme	flavivirus	222:231	arg1	family					233:238	the flavivirus family	218:238	the flavivirus family that can cause fetal neural abnormalities in pregnant women	218:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	7	54	theme	eye	1303:1305	arg1	region					1289:1294	the region	1285:1294	the region of the eye	1285:1305	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	8	55	theme	Dengue	1437:1442	arg1	virus					1444:1448	Dengue virus	1437:1448	Dengue virus (DENV) infection	1437:1465	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	8	55	theme	Dengue	1437:1442	arg1	DENV					1451:1454	DENV	1451:1454	DENV	1451:1454	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	4	56	dep	aegypti	895:901	arg1	mosquitoes					924:933	mosquitoes	924:933	mosquitoes	924:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	1	57	theme	Zika	169:172	arg1	ZIKV					181:184	ZIKV	181:184	ZIKV	181:184	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	57	theme	Zika	169:172	arg1	virus					174:178	Zika virus	169:178	Zika virus (ZIKV)	169:185	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	1	57	theme	Zika	169:172	arg1	virus					207:211	an enveloped RNA virus	190:211	an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women	190:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	6	58	theme	undifferentiated	1105:1120	arg1	cells					1141:1145	undifferentiated and differentiated cells	1105:1145	undifferentiated and differentiated cells	1105:1145	A change in disaccharide composition levels was observed between undifferentiated and differentiated cells.
30698412	10	59	theme	longer	1596:1601	arg1	lengths					1609:1615	longer chain lengths	1596:1615	longer chain lengths	1596:1615	HP of longer chain lengths more strongly promoted activity in ZIKV replication.
30698412	4	60	theme	major	753:757	arg1	GAG					759:761	the major GAG	749:761	the major GAG	749:761	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	60	theme	major	753:757	arg1	sulfate					732:738	Heparan sulfate	724:738	Heparan sulfate (HS)	724:743	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	7	61	theme	keratan	1249:1255	arg1	sulfate					1257:1263	keratan sulfate	1249:1263	keratan sulfate	1249:1263	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	62	theme	acid	1241:1244	arg1	amounts					1219:1225	the amounts	1215:1225	the amounts of hyaluronic acid or keratan sulfate	1215:1263	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	62	theme	acid	1241:1244	arg1	acid					1241:1244	hyaluronic acid	1230:1244	hyaluronic acid	1230:1244	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	62	theme	acid	1241:1244	arg1	sulfate					1257:1263	keratan sulfate	1249:1263	keratan sulfate	1249:1263	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	2	63	theme	ZIKV	479:482	arg1	pathogenesis					484:495	ZIKV pathogenesis	479:495	ZIKV pathogenesis	479:495	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	3	64	theme	tissue	702:707	arg1	GAGs					718:721	tissue specific GAGs	702:721	tissue specific GAGs	702:721	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	0	65	theme	Virus	152:156	arg1	Infection					158:166	Zika Virus Infection	147:166	Zika Virus Infection	147:166	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	5	66	theme	fetal	945:949	arg1	progenitor					958:967	human fetal neural progenitor and differentiated cells	939:992	progenitor	958:967	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	10	67	theme	lengths	1609:1615	arg1	HP					1590:1591	HP	1590:1591	HP of longer chain lengths	1590:1615	HP of longer chain lengths more strongly promoted activity in ZIKV replication.
30698412	4	68	theme	HS-6-sulfo	778:787	arg1	differences					851:861	the main differences	842:861	the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	842:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	68	theme	HS-6-sulfo	778:787	arg1	levels					768:773	levels	768:773	levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides	768:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	6	69	theme	composition	1065:1075	arg1	levels					1077:1082	disaccharide composition levels	1052:1082	disaccharide composition levels	1052:1082	A change in disaccharide composition levels was observed between undifferentiated and differentiated cells.
30698412	11	70	from	pathogenesis	1739:1750	arg1	antivirals					1775:1784	carbohydrate-based antivirals	1756:1784	carbohydrate-based antivirals against flaviviral infection	1756:1813	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	11	70	from	pathogenesis	1739:1750	arg1	understanding					1701:1713	our understanding	1697:1713	our understanding of role of GAGs in ZIKV pathogenesis	1697:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	10	71	theme	ZIKV	1652:1655	arg1	replication					1657:1667	ZIKV replication	1652:1667	ZIKV replication	1652:1667	HP of longer chain lengths more strongly promoted activity in ZIKV replication.
30698412	4	72	theme	HS	790:791	arg1	0S					793:794	HS 0S	790:794	HS 0S (unsulfated HS)	790:810	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	72	theme	HS	790:791	arg1	HS					808:809	unsulfated HS	797:809	unsulfated HS	797:809	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	3	73	theme	disaccharide	546:557	arg1	composition					559:569	the GAG disaccharide composition	538:569	the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis	538:673	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	2	74	theme	potential	439:447	arg1	CS					427:428	CS	427:428	CS	427:428	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	74	theme	potential	439:447	arg1	receptor					467:474	a potential host cell surface receptor	437:474	a potential host cell surface receptor	437:474	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	7	75	theme	major	1200:1204	arg1	GAG					1206:1208	the major GAG	1196:1208	the major GAG	1196:1208	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	7	75	theme	major	1200:1204	arg1	CS					1189:1190	CS	1189:1190	CS	1189:1190	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	4	76	theme	CS	817:818	arg1	disaccharides					823:835	CS 4S disaccharides	817:835	CS 4S disaccharides	817:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	6	77	from	change	1042:1047	arg1	levels					1077:1082	disaccharide composition levels	1052:1082	disaccharide composition levels	1052:1082	A change in disaccharide composition levels was observed between undifferentiated and differentiated cells.
30698412	3	78	theme	GAGs	718:721	arg1	role					694:697	the role	690:697	the role of tissue specific GAGs	690:721	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	0	79	theme	in	92:93	arg1	Evaluation					101:110	in Vitro Evaluation	92:110	in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection	92:166	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	4	80	from	GAG	759:761	arg1	composition					874:884	the GAG composition	866:884	the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	866:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	0	81	theme	Heparin	115:121	arg1	Evaluation					101:110	in Vitro Evaluation	92:110	in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection	92:166	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	0	81	theme	Heparin	115:121	arg1	Pathogenesis					75:86	Zika Virus Pathogenesis	64:86	Zika Virus Pathogenesis	64:86	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	2	82	theme	cell	454:457	arg1	CS					427:428	CS	427:428	CS	427:428	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	82	theme	cell	454:457	arg1	receptor					467:474	a potential host cell surface receptor	437:474	a potential host cell surface receptor	437:474	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	8	83	theme	various	1342:1348	arg1	structures					1350:1359	various structures	1342:1359	various structures	1342:1359	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	4	84	theme	4S	820:821	arg1	disaccharides					823:835	CS 4S disaccharides	817:835	CS 4S disaccharides	817:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	0	85	theme	Compositional	18:30	arg1	Analysis					32:39	Glycosaminoglycan Compositional Analysis	0:39	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.	0:167	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	5	86	theme	differentiated	973:986	arg1	cells					988:992	human fetal neural progenitor and differentiated cells	939:992	cells	988:992	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	0	87	dep	in	92:93	arg1	Vitro					95:99	Vitro	95:99	Vitro	95:99	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	7	88	theme	eyes	1183:1186	arg1	regions					1161:1167	different regions	1151:1167	different regions of the bovine eyes	1151:1186	In different regions of the bovine eyes, CS was the major GAG, and the amounts of hyaluronic acid or keratan sulfate varied depending on the region of the eye.
30698412	5	89	dep	HS	995:996	arg1	4S					1008:1009	4S	1008:1009	4S	1008:1009	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	5	89	dep	HS	995:996	arg1	0S					998:999	0S	998:999	0S	998:999	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	11	90	theme	role	1718:1721	arg1	antivirals					1775:1784	carbohydrate-based antivirals	1756:1784	carbohydrate-based antivirals against flaviviral infection	1756:1813	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	11	90	theme	role	1718:1721	arg1	understanding					1701:1713	our understanding	1697:1713	our understanding of role of GAGs in ZIKV pathogenesis	1697:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	5	91	theme	main	1020:1023	arg1	HS					995:996	HS 0S and CS 4S	995:1009	HS	995:996	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	5	91	theme	main	1020:1023	arg1	disaccharides					1025:1037	the main disaccharides	1016:1037	the main disaccharides	1016:1037	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	5	91	theme	main	1020:1023	arg1	CS					1005:1006	CS	1005:1006	CS	1005:1006	In human fetal neural progenitor and differentiated cells, HS 0S and CS 4S were the main disaccharides.
30698412	2	92	theme	chondroitin	385:395	arg1	CS					406:407	CS	406:407	CS	406:407	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	92	theme	chondroitin	385:395	arg1	sulfate					397:403	human placental chondroitin sulfate	369:403	human placental chondroitin sulfate (CS)	369:408	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	0	93	theme	Tissues	53:59	arg1	Analysis					32:39	Glycosaminoglycan Compositional Analysis	0:39	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.	0:167	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	8	94	theme	in	1392:1393	arg1	activity					1411:1418	their potential in vitro antiviral activity	1376:1418	their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells	1376:1479	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	3	95	theme	biological	580:589	arg1	tissues					591:597	other biological tissues	574:597	other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues)	574:652	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	2	96	theme	human	369:373	arg1	CS					406:407	CS	406:407	CS	406:407	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	2	96	theme	human	369:373	arg1	sulfate					397:403	human placental chondroitin sulfate	369:403	human placental chondroitin sulfate (CS)	369:408	Previously, we established that ZIKV-EP (envelope protein) binds to human placental chondroitin sulfate (CS), suggesting that CS may be a potential host cell surface receptor in ZIKV pathogenesis.
30698412	0	97	theme	Zika	64:67	arg1	Pathogenesis					75:86	Zika Virus Pathogenesis	64:86	Zika Virus Pathogenesis	64:86	Glycosaminoglycan Compositional Analysis of Relevant Tissues in Zika Virus Pathogenesis and in Vitro Evaluation of Heparin as an Antiviral against Zika Virus Infection.
30698412	1	98	theme	pregnant	285:292	arg1	women					294:298	pregnant women	285:298	pregnant women	285:298	Zika virus (ZIKV) is an enveloped RNA virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women.
30698412	8	99	from	activity	1411:1418	arg1	cells					1475:1479	Vero cells	1470:1479	Vero cells	1470:1479	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	8	100	theme	antiviral	1401:1409	arg1	activity					1411:1418	their potential in vitro antiviral activity	1376:1418	their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells	1376:1479	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	4	101	theme	disaccharides	823:835	arg1	differences					851:861	the main differences	842:861	the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	842:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	4	101	theme	disaccharides	823:835	arg1	levels					768:773	levels	768:773	levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides	768:835	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	8	102	dep	in	1392:1393	arg1	vitro					1395:1399	vitro	1395:1399	vitro	1395:1399	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	4	103	from	differences	851:861	arg1	composition					874:884	the GAG composition	866:884	the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes	866:933	Heparan sulfate (HS) was the major GAG, and levels of HS-6-sulfo, HS 0S (unsulfated HS), and CS 4S disaccharides were the main differences in the GAG composition of Aedes aegypti and Aedes albopictus mosquitoes.
30698412	11	104	theme	flaviviral	1794:1803	arg1	infection					1805:1813	flaviviral infection	1794:1813	flaviviral infection	1794:1813	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30698412	6	105	theme	differentiated	1126:1139	arg1	cells					1141:1145	undifferentiated and differentiated cells	1105:1145	undifferentiated and differentiated cells	1105:1145	A change in disaccharide composition levels was observed between undifferentiated and differentiated cells.
30698412	8	106	theme	Vero	1470:1473	arg1	cells					1475:1479	Vero cells	1470:1479	Vero cells	1470:1479	Next, we examined heparin (HP) of various structures to investigate their potential in vitro antiviral activity against ZIKV and Dengue virus (DENV) infection in Vero cells.
30698412	3	107	dep	tissues	591:597	arg1	mosquitoes					606:615	mosquitoes	606:615	mosquitoes	606:615	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	3	107	dep	tissues	591:597	arg1	cells					630:634	fetal brain cells	618:634	fetal brain cells	618:634	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	3	107	dep	tissues	591:597	arg1	tissues					645:651	eye tissues	641:651	eye tissues	641:651	In this study, we further characterized the GAG disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in ZIKV pathogenesis to investigate the role of tissue specific GAGs.
30698412	11	108	theme	GAGs	1726:1729	arg1	role					1718:1721	role	1718:1721	role of GAGs in ZIKV pathogenesis	1718:1750	This study further expands our understanding of role of GAGs in ZIKV pathogenesis and carbohydrate-based antivirals against flaviviral infection.
30021397	7	0	theme	wound	1198:1202	arg1	dressings					1204:1212	the wound dressings	1194:1212	the wound dressings	1194:1212	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	1	1	theme	evaporation	293:303	arg1	process					305:311	evaporation process	293:311	evaporation process	293:311	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	2	2	dep	Fourier	417:423	arg1	transform					425:433	transform	425:433	transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing	425:565	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	8	3	theme	potential	1419:1427	arg1	candidate					1429:1437	a potential candidate	1417:1437	a potential candidate as the wound dressing	1417:1459	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	8	4	theme	50	1318:1319	arg1	%					1320:1320	%	1320:1320	%	1320:1320	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	0	5	theme	composite	86:94	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	4	6	theme	SPI	746:748	arg1	content					750:756	the SPI content	742:756	the SPI content	742:756	The tensile strength was in a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa by adjusting the SPI content.
30021397	8	7	theme	healing	1350:1356	arg1	speed					1358:1362	healing speed	1350:1362	healing speed	1350:1362	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	1	8	theme	different	215:223	arg1	contents					229:236	different SPI contents	215:236	different SPI contents	215:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	2	9	theme	electron	495:502	arg1	microscopy					504:513	scanning electron microscopy	486:513	scanning electron microscopy	486:513	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	10	from	Effects	314:320	arg1	structure					348:356	structure	348:356	structure	348:356	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	10	from	Effects	314:320	arg1	properties					371:380	physical properties	362:380	physical properties	362:380	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	8	11	theme	SPI	1322:1324	arg1	content					1326:1332	50% SPI content	1318:1332	50% SPI content	1318:1332	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	5	12	theme	assays	851:856	arg1	series					832:837	a series	830:837	a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement	830:993	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	13	theme	morphology	902:911	arg1	observation					913:923	cell morphology observation	897:923	cell morphology observation	897:923	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	1	14	theme	SPI	225:227	arg1	contents					229:236	different SPI contents	215:236	different SPI contents	215:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	2	15	theme	scanning	486:493	arg1	microscopy					504:513	scanning electron microscopy	486:513	scanning electron microscopy	486:513	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	16	dep	transform	425:433	arg1	infrared					435:442	infrared	435:442	transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing	425:565	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	8	17	theme	%	1320:1320	arg1	content					1326:1332	50% SPI content	1318:1332	50% SPI content	1318:1332	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	6	18	theme	obvious	1082:1088	arg1	hemolysis					1090:1098	obvious hemolysis	1082:1098	obvious hemolysis	1082:1098	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	7	19	theme	resultant	1175:1183	arg1	HCSFs					1185:1189	resultant HCSFs	1175:1189	resultant HCSFs	1175:1189	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	5	20	theme	hemolysis	926:934	arg1	testing					942:948	hemolysis ratio testing	926:948	hemolysis ratio testing	926:948	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	8	21	theme	speed	1358:1362	arg1	efficiency					1395:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	3	22	from	increase	619:626	arg1	content					639:645	the SPI content	631:645	the SPI content	631:645	The HCSFs exhibited a lower swelling ratio with an increase in the SPI content.
30021397	5	23	theme	ratio	936:940	arg1	testing					942:948	hemolysis ratio testing	926:948	hemolysis ratio testing	926:948	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	1	24	theme	/soy	159:162	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	24	theme	/soy	159:162	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	0	25	theme	skin	12:15	arg1	healing					23:29	skin wound healing	12:29	skin wound healing	12:29	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	8	26	theme	regeneration	1382:1393	arg1	efficiency					1395:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	2	27	theme	diffraction	464:474	arg1	patterns					476:483	X-ray diffraction patterns	458:483	X-ray diffraction patterns	458:483	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	8	28	theme	fastest	1342:1348	arg1	efficiency					1395:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	1	29	theme	protein	164:170	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	29	theme	protein	164:170	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	30	theme	isolate	172:178	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	30	theme	isolate	172:178	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	2	31	theme	HCSFs	389:393	arg1	structure					348:356	structure	348:356	structure	348:356	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	31	theme	HCSFs	389:393	arg1	properties					371:380	physical properties	362:380	physical properties	362:380	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	32	theme	kinetics	525:532	arg1	analysis					534:541	swelling kinetics analysis	516:541	swelling kinetics analysis	516:541	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	5	33	theme	cell	897:900	arg1	observation					913:923	cell morphology observation	897:923	cell morphology observation	897:923	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	2	34	theme	SPI	329:331	arg1	content					333:339	the SPI content	325:339	the SPI content	325:339	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	0	35	theme	wound	17:21	arg1	healing					23:29	skin wound healing	12:29	skin wound healing	12:29	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	2	36	theme	swelling	516:523	arg1	analysis					534:541	swelling kinetics analysis	516:541	swelling kinetics analysis	516:541	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	4	37	theme	tensile	652:658	arg1	strength					660:667	The tensile strength	648:667	The tensile strength	648:667	The tensile strength was in a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa by adjusting the SPI content.
30021397	7	38	from	model	1265:1269	arg1	rats					1274:1277	rats	1274:1277	rats	1274:1277	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	1	39	theme	SPI	181:183	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	39	theme	SPI	181:183	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	0	40	theme	soy	34:36	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	7	41	theme	full-thickness	1239:1252	arg1	model					1265:1269	a full-thickness skin wound model	1237:1269	a full-thickness skin wound model in rats	1237:1277	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	5	42	theme	recalcification	962:976	arg1	measurement					983:993	plasma recalcification time measurement	955:993	plasma recalcification time measurement	955:993	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	2	43	theme	content	333:339	arg1	Effects					314:320	Effects	314:320	Effects of the SPI content on the structure and physical properties of the HCSFs	314:393	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	44	theme	physical	362:369	arg1	properties					371:380	physical properties	362:380	physical properties	362:380	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	5	45	theme	time	978:981	arg1	measurement					983:993	plasma recalcification time measurement	955:993	plasma recalcification time measurement	955:993	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	1	46	theme	composite	186:194	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	46	theme	composite	186:194	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	0	47	theme	isolate-modified	46:61	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	1	48	theme	solution	271:278	arg1	casting					280:286	solution casting	271:286	solution casting	271:286	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	2	49	theme	mechanical	548:557	arg1	testing					559:565	mechanical testing	548:565	mechanical testing	548:565	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	2	50	theme	X-ray	458:462	arg1	patterns					476:483	X-ray diffraction patterns	458:483	X-ray diffraction patterns	458:483	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	8	51	theme	skin	1377:1380	arg1	regeneration					1382:1393	the best skin regeneration	1368:1393	the best skin regeneration	1368:1393	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	5	52	theme	MTT	869:871	arg1	assay					873:877	MTT assay	869:877	MTT assay	869:877	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	0	53	theme	protein	38:44	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	7	54	theme	HCSFs	1185:1189	arg1	potential					1162:1170	The potential	1158:1170	The potential of resultant HCSFs as the wound dressings	1158:1212	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	3	55	theme	SPI	635:637	arg1	content					639:645	the SPI content	631:645	the SPI content	631:645	The HCSFs exhibited a lower swelling ratio with an increase in the SPI content.
30021397	8	56	theme	best	1372:1375	arg1	regeneration					1382:1393	the best skin regeneration	1368:1393	the best skin regeneration	1368:1393	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	7	57	theme	skin	1254:1257	arg1	model					1265:1269	a full-thickness skin wound model	1237:1269	a full-thickness skin wound model in rats	1237:1277	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	5	58	theme	in	842:843	arg1	assay					873:877	MTT assay	869:877	MTT assay	869:877	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	58	theme	in	842:843	arg1	observation					913:923	cell morphology observation	897:923	cell morphology observation	897:923	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	58	theme	in	842:843	arg1	assays					851:856	in vitro assays	842:856	in vitro assays	842:856	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	58	theme	in	842:843	arg1	assay					890:894	live/dead assay	880:894	live/dead assay	880:894	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	58	theme	in	842:843	arg1	measurement					983:993	plasma recalcification time measurement	955:993	plasma recalcification time measurement	955:993	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	58	theme	in	842:843	arg1	testing					942:948	hemolysis ratio testing	926:948	hemolysis ratio testing	926:948	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	3	59	theme	lower	590:594	arg1	ratio					605:609	a lower swelling ratio	588:609	a lower swelling ratio	588:609	The HCSFs exhibited a lower swelling ratio with an increase in the SPI content.
30021397	5	60	dep	in	842:843	arg1	vitro					845:849	vitro	845:849	vitro	845:849	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	4	61	theme	tunable	678:684	arg1	range					686:690	a tunable range	676:690	a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa	676:727	The tensile strength was in a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa by adjusting the SPI content.
30021397	4	62	from	7.88 ± 3.08	697:707	arg1	range					686:690	a tunable range	676:690	a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa	676:727	The tensile strength was in a tunable range from 7.88 ± 3.08 to 40.44 ± 2.31 MPa by adjusting the SPI content.
30021397	1	63	theme	films	196:200	arg1	series					120:125	a series	118:125	a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	118:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	6	64	dep	cells	1039:1043	arg1	proliferation					1060:1072	proliferation	1060:1072	proliferation	1060:1072	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	6	64	dep	cells	1039:1043	arg1	attachment					1045:1054	attachment	1045:1054	attachment	1045:1054	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	6	64	dep	cells	1039:1043	arg1	cells					1039:1043	L929 cells attachment and proliferation	1034:1072	L929 cells attachment and proliferation	1034:1072	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	8	65	with	HCSFs	1307:1311	arg1	content					1326:1332	50% SPI content	1318:1332	50% SPI content	1318:1332	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	3	66	theme	swelling	596:603	arg1	ratio					605:609	a lower swelling ratio	588:609	a lower swelling ratio	588:609	The HCSFs exhibited a lower swelling ratio with an increase in the SPI content.
30021397	5	67	theme	live/dead	880:888	arg1	assay					890:894	live/dead assay	880:894	live/dead assay	880:894	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	7	68	theme	wound	1259:1263	arg1	model					1265:1269	a full-thickness skin wound model	1237:1269	a full-thickness skin wound model in rats	1237:1277	The potential of resultant HCSFs as the wound dressings was investigated using a full-thickness skin wound model in rats.
30021397	0	69	theme	chitosan	77:84	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	8	70	theme	wound	1446:1450	arg1	dressing					1452:1459	the wound dressing	1442:1459	the wound dressing	1442:1459	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	2	71	dep	structure	348:356	arg1	the					344:346	the	344:346	the	344:346	Effects of the SPI content on the structure and physical properties of the HCSFs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction patterns, scanning electron microscopy, swelling kinetics analysis, and mechanical testing.
30021397	5	72	theme	HCSFs	806:810	arg1	hemocompatibility					781:797	hemocompatibility	781:797	hemocompatibility	781:797	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	5	72	theme	HCSFs	806:810	arg1	Cytocompatibility					759:775	Cytocompatibility	759:775	Cytocompatibility	759:775	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	1	73	theme	hydroxypropyl	130:142	arg1	chitosan					144:151	hydroxypropyl chitosan	130:151	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	73	theme	hydroxypropyl	130:142	arg1	HPCS					154:157	HPCS	154:157	HPCS	154:157	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	6	74	theme	L929	1034:1037	arg1	proliferation					1060:1072	proliferation	1060:1072	proliferation	1060:1072	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	6	74	theme	L929	1034:1037	arg1	attachment					1045:1054	attachment	1045:1054	attachment	1045:1054	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	6	74	theme	L929	1034:1037	arg1	cells					1039:1043	L929 cells attachment and proliferation	1034:1072	L929 cells attachment and proliferation	1034:1072	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	0	75	theme	hydroxypropyl	63:75	arg1	films					96:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	soy protein isolate-modified hydroxypropyl chitosan composite films	34:100	Accelerated skin wound healing by soy protein isolate-modified hydroxypropyl chitosan composite films.
30021397	6	76	theme	good	1112:1115	arg1	cytocompatibility					1117:1133	good cytocompatibility	1112:1133	good cytocompatibility	1112:1133	Results showed that the HCSFs support L929 cells attachment and proliferation without obvious hemolysis, indicating good cytocompatibility and hemocompatibility.
30021397	5	77	theme	plasma	955:960	arg1	measurement					983:993	plasma recalcification time measurement	955:993	plasma recalcification time measurement	955:993	Cytocompatibility and hemocompatibility of the HCSFs were evaluated by a series of in vitro assays, including MTT assay, live/dead assay, cell morphology observation, hemolysis ratio testing, and plasma recalcification time measurement.
30021397	1	78	with	films	196:200	arg1	contents					229:236	different SPI contents	215:236	different SPI contents	215:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	79	theme	chitosan	144:151	arg1	HCSFs					203:207	HCSFs	203:207	HCSFs	203:207	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	1	79	theme	chitosan	144:151	arg1	films					196:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films	130:200	hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents	130:236	In this study, a series of hydroxypropyl chitosan (HPCS)/soy protein isolate (SPI) composite films (HCSFs) with different SPI contents were developed via crosslinking, solution casting, and evaporation process.
30021397	8	80	contain	had	1334:1336	arg1	HCSFs					1307:1311	the HCSFs	1303:1311	the HCSFs with 50% SPI content	1303:1332	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
30021397	8	80	contain	had	1334:1336	arg2	efficiency					1395:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	the fastest healing speed and the best skin regeneration efficiency	1338:1404	Results exhibited that the HCSFs with 50% SPI content had the fastest healing speed and the best skin regeneration efficiency and may be a potential candidate as the wound dressing.
31491651	0	0	theme	orange	101:106	arg1	dye					111:113	reactive orange 16 dye	92:113	reactive orange 16 dye using a response surface methodology approach	92:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	3	1	dep	temperature	665:675	arg1	D					678:678	D	678:678	D: 30-50 °C	678:688	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	4	2	theme	Freundlich	758:767	arg1	model					769:773	Freundlich model	758:773	Freundlich model	758:773	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	1	3	theme	glycol	180:185	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	3	4	dep	dose	577:580	arg1	A					583:583	A	583:583	A: 0.02-0.08 g/L	583:598	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	0	5	theme	reactive	92:99	arg1	dye					111:113	reactive orange 16 dye	92:113	reactive orange 16 dye using a response surface methodology approach	92:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	6	6	from	surface	942:948	arg1	mechanism					913:921	The adsorption mechanism	898:921	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP	898:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	1	7	theme	diglycidyl	187:196	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	0	8	theme	dye	111:113	arg1	adsorption					78:87	adsorption	78:87	adsorption of reactive orange 16 dye using a response surface methodology approach	78:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	3	9	theme	solution	642:649	arg1	pH					651:652	solution pH	642:652	solution pH (C: 4-10)	642:662	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	9	theme	solution	642:649	arg1	C					655:655	C	655:655	C	655:655	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	6	10	from	mechanism	913:921	arg1	surface					942:948	the surface	938:948	the surface of CS-EGDE/TNP	938:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	5	11	theme	CS-EGDE/TNP	847:857	arg1	capacity					835:842	The adsorption capacity	820:842	The adsorption capacity of CS-EGDE/TNP for RO16 dye	820:870	The adsorption capacity of CS-EGDE/TNP for RO16 dye was 1407.4 mg/g at 40 °C.
31491651	3	12	theme	adsorption	533:542	arg1	time					704:707	contact time	696:707	contact time (E: 30-90 min)	696:722	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	12	theme	adsorption	533:542	arg1	concentration					611:623	RO16 dye concentration	602:623	RO16 dye concentration (B: 20-80 mg/L)	602:639	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	12	theme	adsorption	533:542	arg1	parameters					548:557	the adsorption key parameters	529:557	the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min)	529:722	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	12	theme	adsorption	533:542	arg1	pH					651:652	solution pH	642:652	solution pH (C: 4-10)	642:662	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	12	theme	adsorption	533:542	arg1	temperature					665:675	temperature	665:675	temperature (D: 30-50 °C)	665:689	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	12	theme	adsorption	533:542	arg1	dose					577:580	adsorbent dose	567:580	adsorbent dose (A: 0.02-0.08 g/L)	567:599	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	1	13	theme	reactive	299:306	arg1	dye					325:327	reactive orange 16 (RO16) dye	299:327	reactive orange 16 (RO16) dye	299:327	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	6	14	theme	CS-EGDE/TNP	953:963	arg1	surface					942:948	the surface	938:948	the surface of CS-EGDE/TNP	938:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	1	15	theme	ether/TiO2	198:207	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	3	16	dep	B	626:626	arg1	20-80 mg/L					629:638	20-80 mg/L	629:638	20-80 mg/L	629:638	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	7	17	theme	reactive	1192:1199	arg1	dye					1201:1203	acid reactive dye	1187:1203	acid reactive dye	1187:1203	Results supported the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye.
31491651	1	18	theme	nanoparticles	209:221	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	6	19	theme	n-π	1041:1043	arg1	interactions					994:1005	various interactions	986:1005	various interactions such as electrostatic attraction	986:1038	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	19	theme	n-π	1041:1043	arg1	Yoshida					1058:1064	Yoshida	1058:1064	Yoshida	1058:1064	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	19	theme	n-π	1041:1043	arg1	attraction					1029:1038	electrostatic attraction	1015:1038	electrostatic attraction	1015:1038	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	19	theme	n-π	1041:1043	arg1	interaction					1045:1055	n-π interaction	1041:1055	n-π interaction	1041:1055	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	4	20	theme	pseudo-second	779:791	arg1	model					813:817	pseudo-second order (PSO) kinetic model	779:817	pseudo-second order (PSO) kinetic model	779:817	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	7	21	theme	dye	1201:1203	arg1	treatment					1174:1182	the treatment	1170:1182	the treatment of acid reactive dye	1170:1203	Results supported the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye.
31491651	1	22	theme	orange	308:313	arg1	dye					325:327	reactive orange 16 (RO16) dye	299:327	reactive orange 16 (RO16) dye	299:327	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	4	23	theme	adsorption	729:738	arg1	isotherm					740:747	The adsorption isotherm	725:747	The adsorption isotherm	725:747	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	5	24	theme	adsorption	824:833	arg1	capacity					835:842	The adsorption capacity	820:842	The adsorption capacity of CS-EGDE/TNP for RO16 dye	820:870	The adsorption capacity of CS-EGDE/TNP for RO16 dye was 1407.4 mg/g at 40 °C.
31491651	1	25	theme	CS-EGDE/TNP	224:234	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	0	26	theme	glycol	31:36	arg1	ether/TiO2					49:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	0	27	theme	surface	132:138	arg1	approach					152:159	a response surface methodology approach	121:159	a response surface methodology approach	121:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	6	28	from	dye	931:933	arg1	surface					942:948	the surface	938:948	the surface of CS-EGDE/TNP	938:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	3	29	theme	key	544:546	arg1	time					704:707	contact time	696:707	contact time (E: 30-90 min)	696:722	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	29	theme	key	544:546	arg1	concentration					611:623	RO16 dye concentration	602:623	RO16 dye concentration (B: 20-80 mg/L)	602:639	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	29	theme	key	544:546	arg1	parameters					548:557	the adsorption key parameters	529:557	the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min)	529:722	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	29	theme	key	544:546	arg1	pH					651:652	solution pH	642:652	solution pH (C: 4-10)	642:662	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	29	theme	key	544:546	arg1	temperature					665:675	temperature	665:675	temperature (D: 30-50 °C)	665:689	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	29	theme	key	544:546	arg1	dose					577:580	adsorbent dose	567:580	adsorbent dose (A: 0.02-0.08 g/L)	567:599	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	2	30	theme	SEM-EDX	420:426	arg1	techniques					428:437	SEM-EDX techniques	420:437	SEM-EDX techniques	420:437	The CS-EGDE/TNP composite was characterized via BET, XRD, FTIR, and SEM-EDX techniques.
31491651	0	31	theme	chitosan-ethylene	13:29	arg1	ether/TiO2					49:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	0	32	theme	response	123:130	arg1	approach					152:159	a response surface methodology approach	121:159	a response surface methodology approach	121:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	6	33	theme	electrostatic	1015:1027	arg1	attraction					1029:1038	electrostatic attraction	1015:1038	electrostatic attraction	1015:1038	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	34	theme	dye	931:933	arg1	mechanism					913:921	The adsorption mechanism	898:921	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP	898:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	0	35	theme	ether/TiO2	49:58	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2	0:58	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	1	36	theme	RO16	319:322	arg1	dye					325:327	reactive orange 16 (RO16) dye	299:327	reactive orange 16 (RO16) dye	299:327	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	3	37	dep	A	583:583	arg1	0.02-0.08 g/L					586:598	0.02-0.08 g/L	586:598	A: 0.02-0.08 g/L	583:598	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	0	38	theme	diglycidyl	38:47	arg1	ether/TiO2					49:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	chitosan-ethylene glycol diglycidyl ether/TiO2	13:58	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	3	39	dep	C	655:655	arg1	4-10					658:661	4-10	658:661	4-10	658:661	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	0	40	theme	methodology	140:150	arg1	approach					152:159	a response surface methodology approach	121:159	a response surface methodology approach	121:159	Synthesis of chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles for adsorption of reactive orange 16 dye using a response surface methodology approach.
31491651	3	41	theme	RO16	602:605	arg1	concentration					611:623	RO16 dye concentration	602:623	RO16 dye concentration (B: 20-80 mg/L)	602:639	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	41	theme	RO16	602:605	arg1	B					626:626	B	626:626	B	626:626	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	1	42	from	solution	342:349	arg1	removal					288:294	the removal	284:294	the removal of reactive orange 16 (RO16) dye from aqueous solution	284:349	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	1	43	theme	dye	325:327	arg1	removal					288:294	the removal	284:294	the removal of reactive orange 16 (RO16) dye from aqueous solution	284:349	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	4	44	theme	order	793:797	arg1	model					813:817	pseudo-second order (PSO) kinetic model	779:817	pseudo-second order (PSO) kinetic model	779:817	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	3	45	theme	dye	607:609	arg1	concentration					611:623	RO16 dye concentration	602:623	RO16 dye concentration (B: 20-80 mg/L)	602:639	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	45	theme	dye	607:609	arg1	B					626:626	B	626:626	B	626:626	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	46	theme	adsorbent	567:575	arg1	dose					577:580	adsorbent dose	567:580	adsorbent dose (A: 0.02-0.08 g/L)	567:599	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	2	47	theme	CS-EGDE/TNP	356:366	arg1	composite					368:376	The CS-EGDE/TNP composite	352:376	The CS-EGDE/TNP composite	352:376	The CS-EGDE/TNP composite was characterized via BET, XRD, FTIR, and SEM-EDX techniques.
31491651	3	48	dep	D	678:678	arg1	30-50 °C					681:688	30-50 °C	681:688	30-50 °C	681:688	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	49	with	methodology	457:467	arg1	design					492:497	Box-Behnken design	480:497	Box-Behnken design (BBD)	480:503	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	49	with	methodology	457:467	arg1	BBD					500:502	BBD	500:502	BBD	500:502	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	50	dep	E	710:710	arg1	30-90 min					713:721	30-90 min	713:721	E: 30-90 min	710:721	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	51	dep	time	704:707	arg1	E					710:710	E	710:710	E: 30-90 min	710:721	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	52	theme	Response	440:447	arg1	RSM					470:472	RSM	470:472	RSM	470:472	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	52	theme	Response	440:447	arg1	methodology					457:467	Response surface methodology	440:467	Response surface methodology (RSM) with Box-Behnken design (BBD)	440:503	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	1	53	theme	aqueous	334:340	arg1	solution					342:349	aqueous solution	334:349	aqueous solution	334:349	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	4	54	theme	PSO	800:802	arg1	model					813:817	pseudo-second order (PSO) kinetic model	779:817	pseudo-second order (PSO) kinetic model	779:817	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	6	55	theme	various	986:992	arg1	interactions					994:1005	various interactions	986:1005	various interactions such as electrostatic attraction	986:1038	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	55	theme	various	986:992	arg1	attraction					1029:1038	electrostatic attraction	1015:1038	electrostatic attraction	1015:1038	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	6	55	theme	various	986:992	arg1	interaction					1045:1055	n-π interaction	1041:1055	n-π interaction	1041:1055	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	3	56	theme	Box-Behnken	480:490	arg1	design					492:497	Box-Behnken design	480:497	Box-Behnken design (BBD)	480:503	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	56	theme	Box-Behnken	480:490	arg1	BBD					500:502	BBD	500:502	BBD	500:502	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	6	57	theme	adsorption	902:911	arg1	mechanism					913:921	The adsorption mechanism	898:921	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP	898:963	The adsorption mechanism of RO16 dye on the surface of CS-EGDE/TNP can be attributed to various interactions such as electrostatic attraction, n-π interaction, Yoshida H-bonding, and H-bonding.
31491651	4	58	theme	kinetic	805:811	arg1	model					813:817	pseudo-second order (PSO) kinetic model	779:817	pseudo-second order (PSO) kinetic model	779:817	The adsorption isotherm followed Freundlich model and pseudo-second order (PSO) kinetic model.
31491651	7	59	theme	acid	1187:1190	arg1	dye					1201:1203	acid reactive dye	1187:1203	acid reactive dye	1187:1203	Results supported the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye.
31491651	7	60	theme	CS-EGDE/TNP	1131:1141	arg1	use					1124:1126	the potential use	1110:1126	the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye	1110:1203	Results supported the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye.
31491651	3	61	theme	surface	449:455	arg1	RSM					470:472	RSM	470:472	RSM	470:472	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	3	61	theme	surface	449:455	arg1	methodology					457:467	Response surface methodology	440:467	Response surface methodology (RSM) with Box-Behnken design (BBD)	440:503	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
31491651	7	62	theme	potential	1114:1122	arg1	use					1124:1126	the potential use	1110:1126	the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye	1110:1203	Results supported the potential use of CS-EGDE/TNP as effective adsorbent for the treatment of acid reactive dye.
31491651	1	63	theme	Chitosan-ethylene	162:178	arg1	composite					237:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite	162:245	Chitosan-ethylene glycol diglycidyl ether/TiO2 nanoparticles (CS-EGDE/TNP) composite was synthesized to be biosorbent for the removal of reactive orange 16 (RO16) dye from aqueous solution.
31491651	3	64	theme	contact	696:702	arg1	time					704:707	contact time	696:707	contact time (E: 30-90 min)	696:722	Response surface methodology (RSM) with Box-Behnken design (BBD) was applied to optimize the adsorption key parameters such as adsorbent dose (A: 0.02-0.08 g/L), RO16 dye concentration (B: 20-80 mg/L), solution pH (C: 4-10), temperature (D: 30-50 °C), and contact time (E: 30-90 min).
30658124	7	0	theme	potassium-channel	1253:1269	arg1	4-Aminopyridine					1196:1210	4-Aminopyridine	1196:1210	4-Aminopyridine (4AP, molecular weight 94.1146 g/mol)	1196:1248	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	7	0	theme	potassium-channel	1253:1269	arg1	blocker					1271:1277	a potassium-channel blocker	1251:1277	a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration	1251:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	3	1	theme	recovery	503:510	arg1	speeds					482:487	speeds	482:487	speeds of functional recovery	482:510	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	12	2	theme	attractive	2036:2045	arg1	strategy					2047:2054	an attractive strategy	2033:2054	an attractive strategy for nerve repair and regeneration	2033:2088	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	2	theme	attractive	2036:2045	arg1	conduits					1984:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	2	theme	attractive	2036:2045	arg1	delivery					2011:2018	4AP sustained delivery	1997:2018	4AP sustained delivery	1997:2018	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	8	3	from	4AP	1425:1427	arg1	7 days					1442:1447	the first 7 days	1432:1447	the first 7 days	1432:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	12	4	theme	4AP	1997:1999	arg1	strategy					2047:2054	an attractive strategy	2033:2054	an attractive strategy for nerve repair and regeneration	2033:2088	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	4	theme	4AP	1997:1999	arg1	conduits					1984:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	4	theme	4AP	1997:1999	arg1	delivery					2011:2018	4AP sustained delivery	1997:2018	4AP sustained delivery	1997:2018	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	2	5	theme	treatment	251:259	arg1	options					261:267	Current treatment options	243:267	Current treatment options	243:267	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	1	6	theme	patients	178:185	arg1	%					162:162	roughly 2.8%	151:162	roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone	151:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	1	6	theme	patients	178:185	arg1	patients					178:185	all trauma patients	167:185	all trauma patients with an annual cost of 7 billion USD in the U.S. alone	167:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	7	7	theme	4-Aminopyridine	1196:1210	arg1	delivery					1184:1191	the sustained delivery	1170:1191	the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration	1170:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	8	8	from	7 days	1442:1447	arg1	4AP					1425:1427	the encapsulated 4AP	1408:1427	the encapsulated 4AP in the first 7 days	1408:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	8	8	from	7 days	1442:1447	arg1	%					1403:1403	30 ± 2%	1397:1403	30 ± 2% of the encapsulated 4AP in the first 7 days	1397:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	4	9	theme	guidance	623:630	arg1	conduit					632:638	a nerve guidance conduit	615:638	a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction	615:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	4	9	theme	guidance	623:630	arg1	alternative					519:529	an alternative	516:529	an alternative to the conduction effects of electrical stimulation	516:581	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	2	10	theme	surgical	277:284	arg1	intervention					286:297	surgical intervention	277:297	surgical intervention with the use of an autograft	277:326	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	9	11	theme	elevated	1477:1484	arg1	expression					1486:1495	elevated expression	1477:1495	elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor	1477:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	10	12	theme	implantation	1814:1825	arg1	4 weeks					1797:1803	4 weeks	1797:1803	4 weeks of their implantation	1797:1825	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	12	13	theme	sustained	2001:2009	arg1	strategy					2047:2054	an attractive strategy	2033:2054	an attractive strategy for nerve repair and regeneration	2033:2088	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	13	theme	sustained	2001:2009	arg1	conduits					1984:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	13	theme	sustained	2001:2009	arg1	delivery					2011:2018	4AP sustained delivery	1997:2018	4AP sustained delivery	1997:2018	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	4	14	theme	molecule	704:711	arg1	drug					713:716	a small molecule drug	696:716	a small molecule drug that promotes nerve impulse conduction	696:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	11	15	theme	Histological	1828:1839	arg1	evaluations					1841:1851	Histological evaluations	1828:1851	Histological evaluations	1828:1851	Histological evaluations suggest conduit biocompatibility and Schwann cell infiltration and organization within the conduit and lumen.
30658124	9	16	theme	proteins	1504:1511	arg1	expression					1486:1495	elevated expression	1477:1495	elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor	1477:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	5	17	theme	59.3 ± 14.2 μm	963:976	arg1	size					955:958	an average pore size	939:958	an average pore size of 59.3 ± 14.2 μm	939:976	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	5	18	theme	pore	950:953	arg1	size					955:958	an average pore size	939:958	an average pore size of 59.3 ± 14.2 μm	939:976	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	5	19	dep	interconnected	889:902	arg1	longitudinally-aligned					905:926	longitudinally-aligned	905:926	longitudinally-aligned	905:926	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	9	20	dep	derived	1573:1579	arg1	brain					1567:1571	brain	1567:1571	brain	1567:1571	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	9	21	theme	growth	1527:1532	arg1	factor					1534:1539	nerve growth factor	1521:1539	nerve growth factor	1521:1539	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	0	22	theme	molecule	90:97	arg1	delivery					104:111	Small molecule drug delivery	84:111	Small molecule drug delivery	84:111	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	6	23	theme	anisotropic	1020:1030	arg1	behavior					1043:1050	anisotropic mechanical behavior	1020:1050	anisotropic mechanical behavior	1020:1050	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	7	24	dep	4-Aminopyridine	1196:1210	arg1	4AP					1213:1215	4AP	1213:1215	4AP	1213:1215	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	7	24	dep	4-Aminopyridine	1196:1210	arg1	94.1146 g/mol					1235:1247	molecular weight 94.1146 g/mol	1218:1247	molecular weight 94.1146 g/mol	1218:1247	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	9	25	theme	neurotrophic	1581:1592	arg1	factor					1594:1599	brain derived neurotrophic factor	1567:1599	brain derived neurotrophic factor	1567:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	7	26	theme	weight	1228:1233	arg1	4AP					1213:1215	4AP	1213:1215	4AP	1213:1215	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	7	26	theme	weight	1228:1233	arg1	94.1146 g/mol					1235:1247	molecular weight 94.1146 g/mol	1218:1247	molecular weight 94.1146 g/mol	1218:1247	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	12	27	theme	nerve	1969:1973	arg1	strategy					2047:2054	an attractive strategy	2033:2054	an attractive strategy for nerve repair and regeneration	2033:2088	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	27	theme	nerve	1969:1973	arg1	conduits					1984:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	27	theme	nerve	1969:1973	arg1	delivery					2011:2018	4AP sustained delivery	1997:2018	4AP sustained delivery	1997:2018	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	0	28	dep	delivery	104:111	arg1	composites					38:47	Aligned microchannel polymer-nanotube composites	0:47	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration	0:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	6	29	theme	similar	1099:1105	arg1	behavior					1043:1050	anisotropic mechanical behavior	1020:1050	anisotropic mechanical behavior	1020:1050	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	3	30	theme	nerve	373:377	arg1	conduits					388:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	4	31	theme	nerve	732:736	arg1	conduction					746:755	nerve impulse conduction	732:755	nerve impulse conduction	732:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	10	32	theme	in	1645:1646	arg1	studies					1653:1659	Preliminary in vivo studies	1633:1659	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats	1633:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	0	33	theme	Aligned	0:6	arg1	composites					38:47	Aligned microchannel polymer-nanotube composites	0:47	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration	0:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	7	34	theme	regeneration	1339:1350	arg1	rate					1325:1328	the rate	1321:1328	the rate of nerve regeneration	1321:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	4	35	theme	conduction	538:547	arg1	effects					549:555	the conduction effects	534:555	the conduction effects of electrical stimulation	534:581	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	8	36	theme	%	1403:1403	arg1	total					1388:1392	a total	1386:1392	a total of 30 ± 2% of the encapsulated 4AP in the first 7 days	1386:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	10	37	theme	sciatic	1681:1687	arg1	nerve					1689:1693	critical-sized sciatic nerve	1666:1693	a critical-sized sciatic nerve defect in Wistar rats	1664:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	0	38	theme	polymer-nanotube	21:36	arg1	composites					38:47	Aligned microchannel polymer-nanotube composites	0:47	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration	0:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	1	39	theme	nerve	125:129	arg1	injury					131:136	Peripheral nerve injury	114:136	Peripheral nerve injury	114:136	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	5	40	theme	interconnected	889:902	arg1	pores					928:932	interconnected, longitudinally-aligned pores	889:932	interconnected, longitudinally-aligned pores	889:932	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	3	41	theme	stem	398:401	arg1	therapies					408:416	stem cell therapies	398:416	stem cell therapies	398:416	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	3	42	theme	electrical	433:442	arg1	stimulation					444:454	transient electrical stimulation	423:454	transient electrical stimulation	423:454	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	7	43	theme	sustained	1174:1182	arg1	delivery					1184:1191	the sustained delivery	1170:1191	the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration	1170:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	11	44	dep	conduit	1944:1950	arg1	the					1940:1942	the	1940:1942	the	1940:1942	Histological evaluations suggest conduit biocompatibility and Schwann cell infiltration and organization within the conduit and lumen.
30658124	9	45	theme	Human	1450:1454	arg1	cells					1464:1468	Human Schwann cells	1450:1468	Human Schwann cells	1450:1468	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	5	46	with	conduit	876:882	arg1	size					955:958	an average pore size	939:958	an average pore size of 59.3 ± 14.2 μm	939:976	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	5	46	with	conduit	876:882	arg1	pores					928:932	interconnected, longitudinally-aligned pores	889:932	interconnected, longitudinally-aligned pores	889:932	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	8	47	theme	4AP	1425:1427	arg1	4AP					1425:1427	the encapsulated 4AP	1408:1427	the encapsulated 4AP in the first 7 days	1408:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	8	47	theme	4AP	1425:1427	arg1	%					1403:1403	30 ± 2%	1397:1403	30 ± 2% of the encapsulated 4AP in the first 7 days	1397:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	2	48	theme	autograft	318:326	arg1	use					308:310	the use	304:310	the use of an autograft	304:326	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	5	49	theme	drug-loaded	809:819	arg1	nanotubes					832:840	drug-loaded halloysite nanotubes	809:840	drug-loaded halloysite nanotubes (HNT)	809:846	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	5	49	theme	drug-loaded	809:819	arg1	HNT					843:845	HNT	843:845	HNT	843:845	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	10	50	from	studies	1653:1659	arg1	defect					1695:1700	a critical-sized sciatic nerve defect	1664:1700	a critical-sized sciatic nerve defect in Wistar rats	1664:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	4	51	with	conduit	632:638	arg1	microchannels					653:665	aligned microchannels	645:665	aligned microchannels	645:665	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	10	52	dep	in	1645:1646	arg1	vivo					1648:1651	vivo	1648:1651	vivo	1648:1651	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	1	53	theme	billion	212:218	arg1	USD					220:222	7 billion USD	210:222	7 billion USD	210:222	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	1	54	with	patients	178:185	arg1	cost					202:205	an annual cost	192:205	an annual cost of 7 billion USD in the U.S. alone	192:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	9	55	theme	dependent	1615:1623	arg1	manner					1625:1630	a 4AP dose dependent manner	1604:1630	a 4AP dose dependent manner	1604:1630	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	5	56	theme	foam-like	866:874	arg1	conduit					876:882	a foam-like conduit	864:882	a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm	864:976	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	12	57	theme	nerve	2060:2064	arg1	repair					2066:2071	nerve repair	2060:2071	nerve repair	2060:2071	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	6	58	theme	native	1118:1123	arg1	nerve					1136:1140	native peripheral nerve	1118:1140	native peripheral nerve	1118:1140	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	5	59	theme	biodegradable	760:772	arg1	structure					783:791	A biodegradable chitosan structure	758:791	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT)	758:846	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	3	60	theme	functional	492:501	arg1	recovery					503:510	functional recovery	492:510	functional recovery	492:510	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	10	61	from	defect	1695:1700	arg1	rats					1712:1715	Wistar rats	1705:1715	Wistar rats	1705:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	4	62	theme	stimulation	571:581	arg1	effects					549:555	the conduction effects	534:555	the conduction effects of electrical stimulation	534:581	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	10	63	theme	Wistar	1705:1710	arg1	rats					1712:1715	Wistar rats	1705:1715	Wistar rats	1705:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	1	64	theme	trauma	171:176	arg1	patients					178:185	all trauma patients	167:185	all trauma patients with an annual cost of 7 billion USD in the U.S. alone	167:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	0	65	theme	nerve	64:68	arg1	regeneration					70:81	peripheral nerve regeneration	53:81	peripheral nerve regeneration	53:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	6	66	theme	mechanical	1032:1041	arg1	behavior					1043:1050	anisotropic mechanical behavior	1020:1050	anisotropic mechanical behavior	1020:1050	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	6	67	with	composite	991:999	arg1	HNTs					1006:1009	HNTs	1006:1009	HNTs	1006:1009	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	4	68	theme	aligned	645:651	arg1	microchannels					653:665	aligned microchannels	645:665	aligned microchannels	645:665	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	5	69	theme	chitosan	774:781	arg1	structure					783:791	A biodegradable chitosan structure	758:791	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT)	758:846	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	9	70	theme	Schwann	1456:1462	arg1	cells					1464:1468	Human Schwann cells	1450:1468	Human Schwann cells	1450:1468	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	1	71	theme	annual	195:200	arg1	cost					202:205	an annual cost	192:205	an annual cost of 7 billion USD in the U.S. alone	192:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	6	72	theme	aligned	983:989	arg1	composite					991:999	The aligned composite	979:999	The aligned composite with HNTs	979:1009	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	4	73	theme	nerve	617:621	arg1	conduit					632:638	a nerve guidance conduit	615:638	a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction	615:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	4	73	theme	nerve	617:621	arg1	alternative					519:529	an alternative	516:529	an alternative to the conduction effects of electrical stimulation	516:581	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	11	74	theme	Schwann	1890:1896	arg1	cell					1898:1901	Schwann cell	1890:1901	Schwann cell infiltration	1890:1914	Histological evaluations suggest conduit biocompatibility and Schwann cell infiltration and organization within the conduit and lumen.
30658124	11	75	theme	conduit	1861:1867	arg1	biocompatibility					1869:1884	conduit biocompatibility	1861:1884	conduit biocompatibility	1861:1884	Histological evaluations suggest conduit biocompatibility and Schwann cell infiltration and organization within the conduit and lumen.
30658124	9	76	theme	key	1500:1502	arg1	factor					1594:1599	brain derived neurotrophic factor	1567:1599	brain derived neurotrophic factor	1567:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	9	76	theme	key	1500:1502	arg1	factor					1534:1539	nerve growth factor	1521:1539	nerve growth factor	1521:1539	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	9	76	theme	key	1500:1502	arg1	proteins					1504:1511	key proteins	1500:1511	key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor	1500:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	9	76	theme	key	1500:1502	arg1	protein					1549:1555	myelin protein zero	1542:1560	myelin protein zero	1542:1560	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	10	77	theme	conduit	1727:1733	arg1	suturability					1735:1746	conduit suturability	1727:1746	conduit suturability	1727:1746	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	4	78	theme	small	698:702	arg1	drug					713:716	a small molecule drug	696:716	a small molecule drug that promotes nerve impulse conduction	696:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	2	79	theme	Current	243:249	arg1	options					261:267	Current treatment options	243:267	Current treatment options	243:267	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	1	80	from	cost	202:205	arg1	U.S.					231:234	the U.S.	227:234	the U.S. alone	227:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	5	81	theme	average	942:948	arg1	size					955:958	an average pore size	939:958	an average pore size of 59.3 ± 14.2 μm	939:976	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	6	82	theme	Young	1059:1063	arg1	modulus					1067:1073	a Young's modulus	1057:1073	a Young's modulus of 0.33 ± 0.1 MPa	1057:1091	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	4	83	theme	drug	713:716	arg1	release					685:691	the sustained release	671:691	the sustained release of a small molecule drug that promotes nerve impulse conduction	671:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	0	84	theme	Small	84:88	arg1	delivery					104:111	Small molecule drug delivery	84:111	Small molecule drug delivery	84:111	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	9	85	theme	nerve	1521:1525	arg1	factor					1534:1539	nerve growth factor	1521:1539	nerve growth factor	1521:1539	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	9	86	theme	derived	1573:1579	arg1	factor					1594:1599	brain derived neurotrophic factor	1567:1599	brain derived neurotrophic factor	1567:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	4	87	theme	sustained	675:683	arg1	release					685:691	the sustained release	671:691	the sustained release of a small molecule drug that promotes nerve impulse conduction	671:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	0	88	theme	drug	99:102	arg1	delivery					104:111	Small molecule drug delivery	84:111	Small molecule drug delivery	84:111	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	2	89	with	intervention	286:297	arg1	use					308:310	the use	304:310	the use of an autograft	304:326	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	7	90	theme	molecular	1218:1226	arg1	4AP					1213:1215	4AP	1213:1215	4AP	1213:1215	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	7	90	theme	molecular	1218:1226	arg1	94.1146 g/mol					1235:1247	molecular weight 94.1146 g/mol	1218:1247	molecular weight 94.1146 g/mol	1218:1247	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	12	91	theme	guidance	1975:1982	arg1	strategy					2047:2054	an attractive strategy	2033:2054	an attractive strategy for nerve repair and regeneration	2033:2088	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	91	theme	guidance	1975:1982	arg1	conduits					1984:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits	1963:1991	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	12	91	theme	guidance	1975:1982	arg1	delivery					2011:2018	4AP sustained delivery	1997:2018	4AP sustained delivery	1997:2018	These nerve guidance conduits and 4AP sustained delivery may serve as an attractive strategy for nerve repair and regeneration.
30658124	9	92	theme	myelin	1542:1547	arg1	protein					1549:1555	myelin protein zero	1542:1560	myelin protein zero	1542:1560	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	10	93	theme	ambulatory	1774:1783	arg1	forces					1785:1790	ambulatory forces	1774:1790	ambulatory forces	1774:1790	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	8	94	theme	conduit	1357:1363	arg1	formulation					1365:1375	The conduit formulation	1353:1375	The conduit formulation	1353:1375	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	10	95	theme	nerve	1689:1693	arg1	defect					1695:1700	a critical-sized sciatic nerve defect	1664:1700	a critical-sized sciatic nerve defect in Wistar rats	1664:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	3	96	theme	transient	423:431	arg1	stimulation					444:454	transient electrical stimulation	423:454	transient electrical stimulation	423:454	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	5	97	theme	halloysite	821:830	arg1	nanotubes					832:840	drug-loaded halloysite nanotubes	809:840	drug-loaded halloysite nanotubes (HNT)	809:846	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	5	97	theme	halloysite	821:830	arg1	HNT					843:845	HNT	843:845	HNT	843:845	A biodegradable chitosan structure reinforced with drug-loaded halloysite nanotubes (HNT) was formed into a foam-like conduit with interconnected, longitudinally-aligned pores with an average pore size of 59.3 ± 14.2 μm.
30658124	7	98	theme	nerve	1333:1337	arg1	regeneration					1339:1350	nerve regeneration	1333:1350	nerve regeneration	1333:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	3	99	theme	guidance	379:386	arg1	conduits					388:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	10	100	theme	Preliminary	1633:1643	arg1	studies					1653:1659	Preliminary in vivo studies	1633:1659	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats	1633:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	1	101	theme	Peripheral	114:123	arg1	injury					131:136	Peripheral nerve injury	114:136	Peripheral nerve injury	114:136	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	6	102	theme	0.33 ± 0.1 MPa	1078:1091	arg1	modulus					1067:1073	a Young's modulus	1057:1073	a Young's modulus of 0.33 ± 0.1 MPa	1057:1091	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	3	103	theme	Engineered	362:371	arg1	conduits					388:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits	362:395	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	4	104	theme	electrical	560:569	arg1	stimulation					571:581	electrical stimulation	560:581	electrical stimulation	560:581	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	4	105	theme	impulse	738:744	arg1	conduction					746:755	nerve impulse conduction	732:755	nerve impulse conduction	732:755	As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction.
30658124	10	106	theme	critical-sized	1666:1679	arg1	nerve					1689:1693	critical-sized sciatic nerve	1666:1693	a critical-sized sciatic nerve defect in Wistar rats	1664:1715	Preliminary in vivo studies in a critical-sized sciatic nerve defect in Wistar rats confirmed conduit suturability and strength to withstand ambulatory forces over 4 weeks of their implantation.
30658124	0	107	theme	peripheral	53:62	arg1	regeneration					70:81	peripheral nerve regeneration	53:81	peripheral nerve regeneration	53:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	3	108	theme	cell	403:406	arg1	therapies					408:416	stem cell therapies	398:416	stem cell therapies	398:416	Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery.
30658124	7	109	theme	alternative	1298:1308	arg1	factor					1291:1296	a growth factor	1282:1296	a growth factor alternative to enhance the rate of nerve regeneration	1282:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	8	110	theme	encapsulated	1412:1423	arg1	4AP					1425:1427	the encapsulated 4AP	1408:1427	the encapsulated 4AP in the first 7 days	1408:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	11	111	theme	cell	1898:1901	arg1	infiltration					1903:1914	Schwann cell infiltration	1890:1914	Schwann cell infiltration	1890:1914	Histological evaluations suggest conduit biocompatibility and Schwann cell infiltration and organization within the conduit and lumen.
30658124	7	112	theme	growth	1284:1289	arg1	factor					1291:1296	a growth factor	1282:1296	a growth factor alternative to enhance the rate of nerve regeneration	1282:1350	This manuscript reports on the sustained delivery of 4-Aminopyridine (4AP, molecular weight 94.1146 g/mol), a potassium-channel blocker as a growth factor alternative to enhance the rate of nerve regeneration.
30658124	2	113	theme	associated	337:346	arg1	shortcomings					348:359	associated shortcomings	337:359	associated shortcomings	337:359	Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings.
30658124	1	114	theme	USD	220:222	arg1	cost					202:205	an annual cost	192:205	an annual cost of 7 billion USD in the U.S. alone	192:240	Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone.
30658124	9	115	link	derived	1573:1579	arg1	factor					1594:1599	brain derived neurotrophic factor	1567:1599	brain derived neurotrophic factor	1567:1599	Human Schwann cells showed elevated expression of key proteins such as nerve growth factor, myelin protein zero, and brain derived neurotrophic factor in a 4AP dose dependent manner.
30658124	6	116	theme	peripheral	1125:1134	arg1	nerve					1136:1140	native peripheral nerve	1118:1140	native peripheral nerve	1118:1140	The aligned composite with HNTs produced anisotropic mechanical behavior with a Young's modulus of 0.33 ± 0.1 MPa, very similar to that of native peripheral nerve.
30658124	8	117	theme	first	1436:1440	arg1	7 days					1442:1447	the first 7 days	1432:1447	the first 7 days	1432:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30658124	0	118	theme	microchannel	8:19	arg1	composites					38:47	Aligned microchannel polymer-nanotube composites	0:47	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration	0:81	Aligned microchannel polymer-nanotube composites for peripheral nerve regeneration: Small molecule drug delivery.
30658124	8	119	from	%	1403:1403	arg1	7 days					1442:1447	the first 7 days	1432:1447	the first 7 days	1432:1447	The conduit formulation released a total of 30 ± 2% of the encapsulated 4AP in the first 7 days.
30695726	0	0	theme	β-cyclodextrin/chitosan/hexamethylenetetramine	57:102	arg1	beads					112:116	β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads	57:116	β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components	57:158	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	8	1	theme	low-cost	1497:1504	arg1	regeneration					1512:1523	low-cost, easy regeneration	1497:1523	low-cost, easy regeneration	1497:1523	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	3	2	theme	capacity	566:573	arg1	removal					582:588	The maximum capacity Cr(VI) removal	554:588	The maximum capacity Cr(VI) removal	554:588	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	1	3	theme	non-toxic	266:274	arg1	beads					301:305	a novel and non-toxic chitosan-based composite beads	254:305	a novel and non-toxic chitosan-based composite beads	254:305	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	5	4	theme	non-toxic	910:918	arg1	III					923:925	III	923:925	III	923:925	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	5	4	theme	non-toxic	910:918	arg1	Cr					920:921	the non-toxic Cr	906:921	the non-toxic Cr(III)	906:926	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	3	5	theme	Cr	575:576	arg1	removal					582:588	The maximum capacity Cr(VI) removal	554:588	The maximum capacity Cr(VI) removal	554:588	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	1	6	theme	chitosan-based	276:289	arg1	beads					301:305	a novel and non-toxic chitosan-based composite beads	254:305	a novel and non-toxic chitosan-based composite beads	254:305	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	6	7	theme	column	1077:1082	arg1	testing					1093:1099	fixed-bed column sorption testing	1067:1099	fixed-bed column sorption testing	1067:1099	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	7	8	theme	concentration	1316:1328	arg1	500 mg/L					1330:1337	initial concentration 500 mg/L	1308:1337	initial concentration 500 mg/L	1308:1337	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	0	9	dep	task-specific	123:135	arg1	integrated					138:147	integrated	138:147	integrated	138:147	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	1	10	theme	composite	291:299	arg1	beads					301:305	a novel and non-toxic chitosan-based composite beads	254:305	a novel and non-toxic chitosan-based composite beads	254:305	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	0	11	theme	aerogel	104:110	arg1	beads					112:116	β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads	57:116	β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components	57:158	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	5	12	theme	β-CD	955:958	arg1	components					941:950	the two components	933:950	the two components of β-CD	933:958	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	5	12	theme	β-CD	955:958	arg1	HMTA					964:967	HMTA	964:967	HMTA with task-specific	964:986	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	7	13	theme	β-CD-CS	1221:1227	arg1	HMTA					1229:1232	the β-CD-CS@HMTA	1217:1232	the β-CD-CS@HMTA	1217:1232	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	0	14	theme	task-specific	123:135	arg1	components					149:158	task-specific, integrated components	123:158	task-specific, integrated components	123:158	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	1	15	theme	β-CD-CS	433:439	arg1	β-cyclodextrin/chitosan/hexamethylenetetramine					385:430	β-cyclodextrin/chitosan/hexamethylenetetramine	385:430	β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA)	385:445	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	1	15	theme	β-CD-CS	433:439	arg1	HMTA					441:444	β-CD-CS@HMTA	433:444	β-CD-CS@HMTA	433:444	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	5	16	dep	played	992:997	arg1	VI					829:830	VI	829:830	VI	829:830	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	5	16	dep	played	992:997	arg1	VI					899:900	VI	899:900	VI	899:900	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	7	17	theme	adsorption	1262:1271	arg1	capability					1273:1282	perfect adsorption capability	1254:1282	perfect adsorption capability towards anionic dyes	1254:1303	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	1	18	theme	adsorption	190:199	arg1	efficacy					201:208	the adsorption efficacy	186:208	the adsorption efficacy of hexavalent chromium from aqueous medium	186:251	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	1	19	theme	@	440:440	arg1	β-cyclodextrin/chitosan/hexamethylenetetramine					385:430	β-cyclodextrin/chitosan/hexamethylenetetramine	385:430	β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA)	385:445	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	1	19	theme	@	440:440	arg1	HMTA					441:444	β-CD-CS@HMTA	433:444	β-CD-CS@HMTA	433:444	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	5	20	theme	Cr	826:827	arg1	removal					815:821	efficient removal	805:821	efficient removal of Cr	805:827	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	7	21	theme	perfect	1254:1260	arg1	capability					1273:1282	perfect adsorption capability	1254:1282	perfect adsorption capability towards anionic dyes	1254:1303	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	5	22	theme	resultant	838:846	arg1	sample					848:853	resultant sample	838:853	resultant sample including chemisorption and reduction of Cr	838:897	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	6	23	theme	fixed-bed	1067:1075	arg1	testing					1093:1099	fixed-bed column sorption testing	1067:1099	fixed-bed column sorption testing	1067:1099	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	7	24	theme	anionic	1292:1298	arg1	dyes					1300:1303	anionic dyes	1292:1303	anionic dyes	1292:1303	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	8	25	dep	Cr	1443:1444	arg1	VI					1446:1447	VI	1446:1447	VI	1446:1447	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	2	26	used	used	513:516	arg2	kinetic					473:479	kinetic	473:479	kinetic	473:479	The pseudo- second-order kinetic and Langmuir isotherm model was used to describe the adsorption process.
30695726	2	26	used	used	513:516	arg2	model					503:507	The pseudo- second-order kinetic and Langmuir isotherm model	448:507	model	503:507	The pseudo- second-order kinetic and Langmuir isotherm model was used to describe the adsorption process.
30695726	6	27	theme	breakthrough	1106:1117	arg1	curves					1119:1124	the breakthrough curves	1102:1124	the breakthrough curves	1102:1124	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	0	28	theme	High-efficacy	0:12	arg1	adsorption					14:23	High-efficacy adsorption	0:23	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.	0:159	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	8	29	theme	regeneration	1512:1523	arg1	superiority					1482:1492	its superiority	1478:1492	its superiority of low-cost, easy regeneration and environmental friendly	1478:1550	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	0	30	with	beads	112:116	arg1	components					149:158	task-specific, integrated components	123:158	task-specific, integrated components	123:158	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	5	31	with	components	941:950	arg1	task-specific					974:986	task-specific	974:986	task-specific	974:986	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	8	32	theme	composite	1398:1406	arg1	beads					1408:1412	as-fabricated chitosan-based composite beads	1369:1412	as-fabricated chitosan-based composite beads	1369:1412	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	8	32	theme	composite	1398:1406	arg1	adsorbents					1428:1437	promising adsorbents	1418:1437	promising adsorbents for Cr(VI) and anionic dyes	1418:1465	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	1	33	theme	hexavalent	213:222	arg1	chromium					224:231	hexavalent chromium	213:231	hexavalent chromium	213:231	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	0	34	theme	Cr	28:29	arg1	adsorption					14:23	High-efficacy adsorption	0:23	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.	0:159	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	8	35	theme	promising	1418:1426	arg1	beads					1408:1412	as-fabricated chitosan-based composite beads	1369:1412	as-fabricated chitosan-based composite beads	1369:1412	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	8	35	theme	promising	1418:1426	arg1	adsorbents					1428:1437	promising adsorbents	1418:1437	promising adsorbents for Cr(VI) and anionic dyes	1418:1465	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	1	36	theme	chromium	224:231	arg1	efficacy					201:208	the adsorption efficacy	186:208	the adsorption efficacy of hexavalent chromium from aqueous medium	186:251	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	2	37	theme	isotherm	494:501	arg1	model					503:507	The pseudo- second-order kinetic and Langmuir isotherm model	448:507	model	503:507	The pseudo- second-order kinetic and Langmuir isotherm model was used to describe the adsorption process.
30695726	8	38	theme	environmental	1529:1541	arg1	friendly					1543:1550	environmental friendly	1529:1550	environmental friendly	1529:1550	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	4	39	theme	SEM-EDS	747:753	arg1	techniques					763:772	FT-IR, SEM-EDS and XPS techniques	740:772	FT-IR, SEM-EDS and XPS techniques	740:772	The sorption mechanism of composite was investigated by employing FT-IR, SEM-EDS and XPS techniques.
30695726	4	40	theme	sorption	678:685	arg1	mechanism					687:695	The sorption mechanism	674:695	The sorption mechanism of composite	674:708	The sorption mechanism of composite was investigated by employing FT-IR, SEM-EDS and XPS techniques.
30695726	2	41	theme	adsorption	534:543	arg1	process					545:551	the adsorption process	530:551	the adsorption process	530:551	The pseudo- second-order kinetic and Langmuir isotherm model was used to describe the adsorption process.
30695726	3	42	dep	removal	582:588	arg1	VI					578:579	VI	578:579	VI	578:579	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	4	43	theme	composite	700:708	arg1	mechanism					687:695	The sorption mechanism	674:695	The sorption mechanism of composite	674:708	The sorption mechanism of composite was investigated by employing FT-IR, SEM-EDS and XPS techniques.
30695726	3	44	theme	reported	639:646	arg1	adsorbents					662:671	reported CS derivative adsorbents	639:671	reported CS derivative adsorbents	639:671	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	1	45	theme	aqueous	238:244	arg1	medium					246:251	aqueous medium	238:251	aqueous medium	238:251	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	5	46	theme	Cr	896:897	arg1	reduction					883:891	reduction	883:891	reduction	883:891	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	5	46	theme	Cr	896:897	arg1	chemisorption					865:877	chemisorption	865:877	chemisorption	865:877	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	1	47	theme	task-specific	336:348	arg1	components					350:359	task-specific components	336:359	task-specific components	336:359	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	5	48	with	HMTA	964:967	arg1	task-specific					974:986	task-specific	974:986	task-specific	974:986	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	3	49	theme	CS	648:649	arg1	adsorbents					662:671	reported CS derivative adsorbents	639:671	reported CS derivative adsorbents	639:671	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	8	50	theme	friendly	1543:1550	arg1	superiority					1482:1492	its superiority	1478:1492	its superiority of low-cost, easy regeneration and environmental friendly	1478:1550	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	5	51	theme	efficient	805:813	arg1	removal					815:821	efficient removal	805:821	efficient removal of Cr	805:827	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	8	52	theme	chitosan-based	1383:1396	arg1	beads					1408:1412	as-fabricated chitosan-based composite beads	1369:1412	as-fabricated chitosan-based composite beads	1369:1412	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	8	52	theme	chitosan-based	1383:1396	arg1	adsorbents					1428:1437	promising adsorbents	1418:1437	promising adsorbents for Cr(VI) and anionic dyes	1418:1465	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	1	53	from	medium	246:251	arg1	efficacy					201:208	the adsorption efficacy	186:208	the adsorption efficacy of hexavalent chromium from aqueous medium	186:251	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	6	54	dep	rates	1180:1184	arg1	2					1190:1190	2	1190:1190	2	1190:1190	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	6	54	dep	rates	1180:1184	arg1	1					1187:1187	1	1187:1187	1	1187:1187	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	6	54	dep	rates	1180:1184	arg1	3 mL/min					1196:1203	3 mL/min	1196:1203	3 mL/min	1196:1203	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	8	55	theme	as-fabricated	1369:1381	arg1	beads					1408:1412	as-fabricated chitosan-based composite beads	1369:1412	as-fabricated chitosan-based composite beads	1369:1412	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	8	55	theme	as-fabricated	1369:1381	arg1	adsorbents					1428:1437	promising adsorbents	1418:1437	promising adsorbents for Cr(VI) and anionic dyes	1418:1465	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	7	56	theme	@	1228:1228	arg1	HMTA					1229:1232	the β-CD-CS@HMTA	1217:1232	the β-CD-CS@HMTA	1217:1232	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	3	57	theme	maximum	558:564	arg1	capacity					566:573	The maximum capacity	554:573	The maximum capacity Cr(VI) removal	554:588	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	5	58	theme	adsorption	1025:1034	arg1	process					1036:1042	the adsorption process	1021:1042	the adsorption process	1021:1042	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	0	59	dep	Cr	28:29	arg1	VI					31:32	VI	31:32	VI	31:32	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	5	60	theme	crucial	1001:1007	arg1	role					1009:1012	a crucial role	999:1012	a crucial role	999:1012	It showed that the reason for efficient removal of Cr(VI) onto resultant sample including chemisorption and reduction of Cr(VI) to the non-toxic Cr(III), and the two components of β-CD and HMTA with task-specific had played a crucial role during the adsorption process.
30695726	6	61	theme	Thomas	1146:1151	arg1	model					1153:1157	Thomas model	1146:1157	Thomas model under different flow rates (1, 2 and 3 mL/min)	1146:1204	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	8	62	theme	anionic	1454:1460	arg1	dyes					1462:1465	anionic dyes	1454:1465	anionic dyes	1454:1465	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	6	63	theme	different	1165:1173	arg1	rates					1180:1184	different flow rates	1165:1184	different flow rates (1, 2 and 3 mL/min)	1165:1204	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	6	64	theme	sorption	1084:1091	arg1	testing					1093:1099	fixed-bed column sorption testing	1067:1099	fixed-bed column sorption testing	1067:1099	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	8	65	dep	low-cost	1497:1504	arg1	easy					1507:1510	easy	1507:1510	easy	1507:1510	This research indicated that as-fabricated chitosan-based composite beads are promising adsorbents for Cr(VI) and anionic dyes because of its superiority of low-cost, easy regeneration and environmental friendly.
30695726	0	66	theme	dyes	47:50	arg1	adsorption					14:23	High-efficacy adsorption	0:23	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.	0:159	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	3	67	theme	derivative	651:660	arg1	adsorbents					662:671	reported CS derivative adsorbents	639:671	reported CS derivative adsorbents	639:671	The maximum capacity Cr(VI) removal reached 333.8 mg/g which was superior to most of reported CS derivative adsorbents.
30695726	2	68	theme	Langmuir	485:492	arg1	model					503:507	The pseudo- second-order kinetic and Langmuir isotherm model	448:507	model	503:507	The pseudo- second-order kinetic and Langmuir isotherm model was used to describe the adsorption process.
30695726	1	69	theme	novel	256:260	arg1	beads					301:305	a novel and non-toxic chitosan-based composite beads	254:305	a novel and non-toxic chitosan-based composite beads	254:305	To significantly enhance the adsorption efficacy of hexavalent chromium from aqueous medium, a novel and non-toxic chitosan-based composite beads were prepared by integrating task-specific components into one sample, namely β-cyclodextrin/chitosan/hexamethylenetetramine (β-CD-CS@HMTA).
30695726	0	70	theme	anionic	39:45	arg1	dyes					47:50	anionic dyes	39:50	anionic dyes	39:50	High-efficacy adsorption of Cr(VI) and anionic dyes onto β-cyclodextrin/chitosan/hexamethylenetetramine aerogel beads with task-specific, integrated components.
30695726	7	71	theme	initial	1308:1314	arg1	500 mg/L					1330:1337	initial concentration 500 mg/L	1308:1337	initial concentration 500 mg/L	1308:1337	Moreover, the β-CD-CS@HMTA had also manifested perfect adsorption capability towards anionic dyes in initial concentration 500 mg/L.
30695726	6	72	theme	flow	1175:1178	arg1	rates					1180:1184	different flow rates	1165:1184	different flow rates (1, 2 and 3 mL/min)	1165:1204	Most importantly, for fixed-bed column sorption testing, the breakthrough curves were well fitted by Thomas model under different flow rates (1, 2 and 3 mL/min).
30695726	4	73	theme	FT-IR	740:744	arg1	techniques					763:772	FT-IR, SEM-EDS and XPS techniques	740:772	FT-IR, SEM-EDS and XPS techniques	740:772	The sorption mechanism of composite was investigated by employing FT-IR, SEM-EDS and XPS techniques.
30695726	4	74	theme	XPS	759:761	arg1	techniques					763:772	FT-IR, SEM-EDS and XPS techniques	740:772	FT-IR, SEM-EDS and XPS techniques	740:772	The sorption mechanism of composite was investigated by employing FT-IR, SEM-EDS and XPS techniques.
28967050	1	0	theme	inputs	128:133	arg1	one					107:109	one	107:109	one	107:109	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	1	0	theme	inputs	128:133	arg1	inputs					128:133	the important inputs	114:133	the important inputs for the printing industry	114:159	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	4	1	from	important	514:522	arg1	industry					537:544	the paper industry	527:544	the paper industry	527:544	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	1	2	theme	printing	210:217	arg1	process					219:225	the printing process	206:225	the printing process	206:225	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	4	3	from	industry	537:544	arg1	important					514:522	important	514:522	important	514:522	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	7	4	theme	scales	865:870	arg1	amounts					849:855	different amounts	839:855	different amounts of fish scales	839:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	4	theme	scales	865:870	arg1	scales					865:870	fish scales	860:870	fish scales	860:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	3	5	theme	materials	450:458	arg1	sizing					436:441	sizing	436:441	sizing	436:441	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	3	5	theme	materials	450:458	arg1	coating					424:430	coating	424:430	coating	424:430	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	11	6	theme	coating	1354:1360	arg1	compositions					1362:1373	the initial coating compositions	1342:1373	the initial coating compositions	1342:1373	CONCLUSIONS The coatings obtained were brighter than the initial coating compositions.
28967050	12	7	theme	top	1380:1382	arg1	formulation					1392:1402	The top quality formulation	1376:1402	The top quality formulation	1376:1402	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	12	7	theme	top	1380:1382	arg1	coating					1412:1418	the coating	1408:1418	the coating with 5% medium-sized fish scale particles	1408:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	0	8	theme	paper	80:84	arg1	coating					58:64	surface coating	50:64	surface coating of cellulosic paper	50:84	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	6	9	theme	milling	793:799	arg1	process					801:807	a milling process	791:807	a milling process	791:807	METHODS Fish scales were brought to different sizes by a milling process.
28967050	3	10	dep	coating	424:430	arg1	the					420:422	the	420:422	the	420:422	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	4	11	theme	paper	531:535	arg1	industry					537:544	the paper industry	527:544	the paper industry	527:544	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	0	12	theme	cellulosic	69:78	arg1	paper					80:84	cellulosic paper	69:84	cellulosic paper	69:84	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	0	13	from	material	38:45	arg1	coating					58:64	surface coating	50:64	surface coating of cellulosic paper	50:84	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	7	14	theme	physical	996:1003	arg1	characteristics					1005:1019	physical characteristics	996:1019	physical characteristics	996:1019	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	8	15	theme	2.5	1165:1167	arg1	%					1168:1168	%	1168:1168	%	1168:1168	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	5	16	theme	cosmetic	659:666	arg1	material					668:675	cosmetic material	659:675	cosmetic material	659:675	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	16	theme	cosmetic	659:666	arg1	scales					612:617	Fish scales	607:617	Fish scales	607:617	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	8	17	theme	-10	1169:1171	arg1	%					1168:1168	%	1168:1168	%	1168:1168	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	7	18	theme	fish	860:863	arg1	scales					865:870	fish scales	860:870	fish scales	860:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	4	19	theme	carbon	589:594	arg1	footprint					596:604	a lower carbon footprint	581:604	a lower carbon footprint	581:604	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	3	20	theme	Desired	308:314	arg1	properties					324:333	Desired surface properties	308:333	Desired surface properties	308:333	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	12	21	theme	quality	1384:1390	arg1	formulation					1392:1402	The top quality formulation	1376:1402	The top quality formulation	1376:1402	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	12	21	theme	quality	1384:1390	arg1	coating					1412:1418	the coating	1408:1418	the coating with 5% medium-sized fish scale particles	1408:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	7	22	theme	different	839:847	arg1	amounts					849:855	different amounts	839:855	different amounts of fish scales	839:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	22	theme	different	839:847	arg1	scales					865:870	fish scales	860:870	fish scales	860:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	1	23	theme	printing	143:150	arg1	industry					152:159	the printing industry	139:159	the printing industry	139:159	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	6	24	theme	different	772:780	arg1	sizes					782:786	different sizes	772:786	different sizes	772:786	METHODS Fish scales were brought to different sizes by a milling process.
28967050	8	25	theme	yellowness	1141:1150	arg1	value					1132:1136	the value	1128:1136	the value of yellowness	1128:1150	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	9	26	theme	maximum	1191:1197	arg1	value					1199:1203	The maximum value	1187:1203	The maximum value of brightness	1187:1217	The maximum value of brightness was obtained from a mixture of 10%.
28967050	0	27	theme	filling	30:36	arg1	material					38:45	a filling material	28:45	a filling material in surface coating of cellulosic paper	28:84	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	4	28	theme	biomaterials	484:495	arg1	use					477:479	The use	473:479	The use of biomaterials	473:495	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	5	29	used	used	631:634	arg2	material					649:656	a filling material	639:656	a filling material	639:656	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	29	used	used	631:634	arg2	food					686:689	fish food	681:689	fish food	681:689	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	29	used	used	631:634	arg2	material					668:675	cosmetic material	659:675	cosmetic material	659:675	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	29	used	used	631:634	arg2	determining					707:717	determining	707:717	determining the age of fish	707:733	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	29	used	used	631:634	arg2	scales					612:617	Fish scales	607:617	Fish scales	607:617	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	30	theme	fish	730:733	arg1	age					723:725	the age	719:725	the age of fish	719:733	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	0	31	theme	fish	13:16	arg1	Potential					0:8	Potential	0:8	Potential of fish	0:16	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	1	32	theme	BACKGROUND	87:96	arg1	Paper					98:102	BACKGROUND Paper	87:102	BACKGROUND Paper	87:102	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	9	33	theme	brightness	1208:1217	arg1	value					1199:1203	The maximum value	1187:1203	The maximum value of brightness	1187:1217	The maximum value of brightness was obtained from a mixture of 10%.
28967050	12	34	with	coating	1412:1418	arg1	particles					1452:1460	5% medium-sized fish scale particles	1425:1460	5% medium-sized fish scale particles	1425:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	7	35	with	printings	953:961	arg1	formulations					979:990	formulations	979:990	formulations	979:990	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	35	with	printings	953:961	arg1	characteristics					1005:1019	physical characteristics	996:1019	physical characteristics	996:1019	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	12	36	theme	%	1426:1426	arg1	particles					1452:1460	5% medium-sized fish scale particles	1425:1460	5% medium-sized fish scale particles	1425:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	7	37	from	coatings	908:915	arg1	paper					924:928	raw paper	920:928	raw paper	920:928	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	12	38	theme	medium-sized	1428:1439	arg1	particles					1452:1460	5% medium-sized fish scale particles	1425:1460	5% medium-sized fish scale particles	1425:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	5	39	theme	filling	641:647	arg1	material					649:656	a filling material	639:656	a filling material	639:656	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	39	theme	filling	641:647	arg1	scales					612:617	Fish scales	607:617	Fish scales	607:617	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	7	40	from	printings	953:961	arg1	IGT-C1					966:971	IGT-C1	966:971	IGT-C1	966:971	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	1	41	theme	important	175:183	arg1	brightness					234:243	its brightness	230:243	its brightness	230:243	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	1	41	theme	important	175:183	arg1	parameter					193:201	the most important leading parameter	166:201	the most important leading parameter in the printing process	166:225	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	7	42	theme	coatings	1024:1031	arg1	formulations					979:990	formulations	979:990	formulations	979:990	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	42	theme	coatings	1024:1031	arg1	characteristics					1005:1019	physical characteristics	996:1019	physical characteristics	996:1019	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	3	43	theme	surface	316:322	arg1	properties					324:333	Desired surface properties	308:333	Desired surface properties	308:333	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	5	44	theme	Fish	607:610	arg1	material					649:656	a filling material	639:656	a filling material	639:656	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	44	theme	Fish	607:610	arg1	material					668:675	cosmetic material	659:675	cosmetic material	659:675	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	44	theme	Fish	607:610	arg1	food					686:689	fish food	681:689	fish food	681:689	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	44	theme	Fish	607:610	arg1	scales					612:617	Fish scales	607:617	Fish scales	607:617	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	44	theme	Fish	607:610	arg1	determining					707:717	determining	707:717	determining the age of fish	707:733	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	11	45	theme	initial	1346:1352	arg1	compositions					1362:1373	the initial coating compositions	1342:1373	the initial coating compositions	1342:1373	CONCLUSIONS The coatings obtained were brighter than the initial coating compositions.
28967050	3	46	theme	sizing	436:441	arg1	contents					408:415	the contents	404:415	the contents of the coating and sizing of the materials it contains	404:470	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	3	47	theme	surface	358:364	arg1	roughness					366:374	surface roughness	358:374	surface roughness	358:374	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	12	48	theme	scale	1446:1450	arg1	particles					1452:1460	5% medium-sized fish scale particles	1425:1460	5% medium-sized fish scale particles	1425:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	7	49	theme	adhesion	1078:1085	arg1	etc.					1087:1090	adhesion etc.	1078:1090	adhesion etc. being determined	1078:1107	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	49	theme	adhesion	1078:1085	arg1	brightness					1041:1050	brightness	1041:1050	brightness	1041:1050	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	6	50	theme	Fish	744:747	arg1	scales					749:754	METHODS Fish scales	736:754	METHODS Fish scales	736:754	METHODS Fish scales were brought to different sizes by a milling process.
28967050	12	51	theme	fish	1441:1444	arg1	particles					1452:1460	5% medium-sized fish scale particles	1425:1460	5% medium-sized fish scale particles	1425:1460	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	7	52	theme	raw	920:922	arg1	paper					924:928	raw paper	920:928	raw paper	920:928	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	6	53	theme	METHODS	736:742	arg1	scales					749:754	METHODS Fish scales	736:754	METHODS Fish scales	736:754	METHODS Fish scales were brought to different sizes by a milling process.
28967050	11	54	dep	CONCLUSIONS	1289:1299	arg1	compositions					1362:1373	the initial coating compositions	1342:1373	the initial coating compositions	1342:1373	CONCLUSIONS The coatings obtained were brighter than the initial coating compositions.
28967050	3	55	theme	coating	424:430	arg1	contents					408:415	the contents	404:415	the contents of the coating and sizing of the materials it contains	404:470	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	7	56	theme	Paper	810:814	arg1	formulations					816:827	Paper formulations	810:827	Paper formulations including different amounts of fish scales	810:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	56	theme	Paper	810:814	arg1	amounts					849:855	different amounts	839:855	different amounts of fish scales	839:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	7	56	theme	Paper	810:814	arg1	scales					865:870	fish scales	860:870	fish scales	860:870	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	1	57	theme	leading	185:191	arg1	brightness					234:243	its brightness	230:243	its brightness	230:243	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	1	57	theme	leading	185:191	arg1	parameter					193:201	the most important leading parameter	166:201	the most important leading parameter in the printing process	166:225	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	1	58	from	parameter	193:201	arg1	process					219:225	the printing process	206:225	the printing process	206:225	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	4	59	with	substances	565:574	arg1	footprint					596:604	a lower carbon footprint	581:604	a lower carbon footprint	581:604	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	3	60	dep	contents	408:415	arg1	contains					463:470	contains	463:470	contains	463:470	Desired surface properties and, most importantly, surface roughness can be achieved by changing the contents of the coating and sizing of the materials it contains.
28967050	7	61	theme	fish	891:894	arg1	scales					896:901	fish scales	891:901	fish scales	891:901	Paper formulations including different amounts of fish scales were prepared with fish scales, and coatings on raw paper were subjected to test printings in IGT-C1, with formulations and physical characteristics of coatings such as brightness, lightfastness, strength, adhesion etc. being determined.
28967050	8	62	theme	%	1172:1172	arg1	mixtures					1153:1160	mixtures	1153:1160	mixtures of 2.5%-10%	1153:1172	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	8	62	theme	%	1172:1172	arg1	RESULTS					1110:1116	RESULTS	1110:1116	RESULTS Regarding the value of yellowness	1110:1150	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	0	63	theme	surface	50:56	arg1	coating					58:64	surface coating	50:64	surface coating of cellulosic paper	50:84	Potential of fish scales as a filling material in surface coating of cellulosic paper.
28967050	4	64	theme	lower	583:587	arg1	footprint					596:604	a lower carbon footprint	581:604	a lower carbon footprint	581:604	The use of biomaterials is becoming more important in the paper industry, as they represent substances with a lower carbon footprint.
28967050	12	65	theme	5	1425:1425	arg1	%					1426:1426	%	1426:1426	%	1426:1426	The top quality formulation was the coating with 5% medium-sized fish scale particles.
28967050	5	66	theme	fish	681:684	arg1	food					686:689	fish food	681:689	fish food	681:689	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	5	66	theme	fish	681:684	arg1	scales					612:617	Fish scales	607:617	Fish scales	607:617	Fish scales are already used as a filling material, cosmetic material and fish food, as well as for determining the age of fish.
28967050	8	67	used	used	1181:1184	arg2	mixtures					1153:1160	mixtures	1153:1160	mixtures of 2.5%-10%	1153:1172	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	8	67	used	used	1181:1184	arg2	RESULTS					1110:1116	RESULTS	1110:1116	RESULTS Regarding the value of yellowness	1110:1150	RESULTS Regarding the value of yellowness, mixtures of 2.5%-10% can be used.
28967050	1	68	theme	important	118:126	arg1	inputs					128:133	the important inputs	114:133	the important inputs for the printing industry	114:159	BACKGROUND Paper is one of the important inputs for the printing industry, and the most important leading parameter in the printing process is its brightness.
28967050	9	69	theme	%	1252:1252	arg1	mixture					1239:1245	a mixture	1237:1245	a mixture of 10%	1237:1252	The maximum value of brightness was obtained from a mixture of 10%.
29458101	0	0	theme	full	82:85	arg1	CCD					97:99	24 full factorial CCD	79:99	24 full factorial CCD	79:99	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	3	1	theme	optimal	512:518	arg1	pH					528:529	the optimal working pH	508:529	the optimal working pH for the free pectinase from 5	508:559	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	8	2	theme	gel	1204:1206	arg1	formula					1208:1214	This gel formula	1199:1214	This gel formula	1199:1214	This gel formula may be convenient for immobilization of other industrial enzymes.
29458101	3	3	theme	60°C	671:674	arg1	range					574:578	a broader range	564:578	a broader range	564:578	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	7	4	theme	reusability	1091:1101	arg1	test					1103:1106	The reusability test	1087:1106	The reusability test	1087:1106	The reusability test revealed that 60% of pectinase's original activity was retained after 8 successive cycles.
29458101	2	5	theme	loading	371:377	arg1	time					379:382	loading time	371:382	loading time	371:382	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	6	6	theme	remarkable	998:1007	arg1	increase					1009:1016	a remarkable increase	996:1016	a remarkable increase in enthalpy and free energy	996:1044	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	0	7	from	Optimization	0:11	arg1	beads					70:74	grafted alginate-agar gel beads	44:74	grafted alginate-agar gel beads	44:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	6	8	theme	pectinase	981:989	arg1	immobilization					963:976	immobilization	963:976	immobilization of pectinase	963:989	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	0	9	theme	factorial	87:95	arg1	CCD					97:99	24 full factorial CCD	79:99	24 full factorial CCD	79:99	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	5	10	from	increase	875:882	arg1	half-lives					887:896	half-lives	887:896	half-lives	887:896	Moreover, an increase in half-lives and D-values was achieved.
29458101	5	10	from	increase	875:882	arg1	D-values					902:909	D-values	902:909	D-values	902:909	Moreover, an increase in half-lives and D-values was achieved.
29458101	8	11	theme	enzymes	1273:1279	arg1	immobilization					1238:1251	immobilization	1238:1251	immobilization of other industrial enzymes	1238:1279	This gel formula may be convenient for immobilization of other industrial enzymes.
29458101	0	12	theme	thermodynamic	105:117	arg1	profiling					119:127	thermodynamic profiling	105:127	thermodynamic profiling	105:127	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	8	13	theme	industrial	1262:1271	arg1	enzymes					1273:1279	other industrial enzymes	1256:1279	other industrial enzymes	1256:1279	This gel formula may be convenient for immobilization of other industrial enzymes.
29458101	7	14	theme	original	1141:1148	arg1	activity					1150:1157	pectinase's original activity	1129:1157	pectinase's original activity	1129:1157	The reusability test revealed that 60% of pectinase's original activity was retained after 8 successive cycles.
29458101	7	15	theme	activity	1150:1157	arg1	activity					1150:1157	pectinase's original activity	1129:1157	pectinase's original activity	1129:1157	The reusability test revealed that 60% of pectinase's original activity was retained after 8 successive cycles.
29458101	7	15	theme	activity	1150:1157	arg1	%					1124:1124	60%	1122:1124	60% of pectinase's original activity	1122:1157	The reusability test revealed that 60% of pectinase's original activity was retained after 8 successive cycles.
29458101	3	16	theme	free	539:542	arg1	pectinase					544:552	the free pectinase	535:552	the free pectinase	535:552	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	6	17	from	increase	1009:1016	arg1	enthalpy					1021:1028	enthalpy	1021:1028	enthalpy	1021:1028	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	6	17	from	increase	1009:1016	arg1	energy					1039:1044	free energy	1034:1044	free energy	1034:1044	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	3	18	theme	microbial	717:725	arg1	contamination					727:739	the enzyme's microbial contamination	704:739	the enzyme's microbial contamination	704:739	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	8	19	theme	other	1256:1260	arg1	enzymes					1273:1279	other industrial enzymes	1256:1279	other industrial enzymes	1256:1279	This gel formula may be convenient for immobilization of other industrial enzymes.
29458101	7	20	theme	successive	1180:1189	arg1	cycles					1191:1196	8 successive cycles	1178:1196	8 successive cycles	1178:1196	The reusability test revealed that 60% of pectinase's original activity was retained after 8 successive cycles.
29458101	1	21	theme	gel	299:301	arg1	beads					303:307	gel beads	299:307	gel beads made of alginate and agar	299:333	Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar.
29458101	4	22	theme	immobilized	841:851	arg1	formula					853:859	the immobilized formula	837:859	the immobilized formula	837:859	The thermodynamics studies showed a thermal stability enhancement against high temperature for the immobilized formula.
29458101	3	23	theme	broader	566:572	arg1	range					574:578	a broader range	564:578	a broader range	564:578	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	24	from	range	574:578	arg1	55°C					638:641	55°C	638:641	55°C	638:641	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	6	25	theme	stability	1064:1072	arg1	enhancement					1074:1084	enzyme stability enhancement	1057:1084	enzyme stability enhancement	1057:1084	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	2	26	theme	composite	447:455	arg1	design					457:462	24 full factorial central composite design	421:462	24 full factorial central composite design (CCD)	421:468	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	26	theme	composite	447:455	arg1	CCD					465:467	CCD	465:467	CCD	465:467	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	27	theme	factorial	429:437	arg1	design					457:462	24 full factorial central composite design	421:462	24 full factorial central composite design (CCD)	421:468	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	27	theme	factorial	429:437	arg1	CCD					465:467	CCD	465:467	CCD	465:467	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	28	theme	central	439:445	arg1	design					457:462	24 full factorial central composite design	421:462	24 full factorial central composite design (CCD)	421:468	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	28	theme	central	439:445	arg1	CCD					465:467	CCD	465:467	CCD	465:467	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	3	29	theme	working	520:526	arg1	pH					528:529	the optimal working pH	508:529	the optimal working pH for the free pectinase from 5	508:559	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	2	30	theme	full	424:427	arg1	design					457:462	24 full factorial central composite design	421:462	24 full factorial central composite design (CCD)	421:468	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	2	30	theme	full	424:427	arg1	CCD					465:467	CCD	465:467	CCD	465:467	Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD).
29458101	0	31	theme	grafted	44:50	arg1	beads					70:74	grafted alginate-agar gel beads	44:74	grafted alginate-agar gel beads	44:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	3	32	theme	pH4.5-5.5	583:591	arg1	range					574:578	a broader range	564:578	a broader range	564:578	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	0	33	theme	immobilization	26:39	arg1	Optimization					0:11	Optimization	0:11	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.	0:182	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	0	34	theme	gel	66:68	arg1	beads					70:74	grafted alginate-agar gel beads	44:74	grafted alginate-agar gel beads	44:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	0	35	theme	covalent	159:166	arg1	immobilization					168:181	operational covalent immobilization	147:181	operational covalent immobilization	147:181	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	0	36	from	beads	70:74	arg1	Optimization					0:11	Optimization	0:11	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.	0:182	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	0	37	theme	alginate-agar	52:64	arg1	beads					70:74	grafted alginate-agar gel beads	44:74	grafted alginate-agar gel beads	44:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	6	38	theme	thermodynamic	929:941	arg1	studies					943:949	The thermodynamic studies	925:949	The thermodynamic studies	925:949	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	0	39	theme	operational	147:157	arg1	immobilization					168:181	operational covalent immobilization	147:181	operational covalent immobilization	147:181	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	1	40	attach	derived	214:220	arg1	awamori					250:256	the fungus Aspergillus awamori KX943614	227:265	the fungus Aspergillus awamori KX943614	227:265	Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar.
29458101	1	40	attach	derived	214:220	arg2	honey					208:212	a honey	206:212	a honey derived from the fungus Aspergillus awamori KX943614	206:265	Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar.
29458101	4	41	theme	high	816:819	arg1	temperature					821:831	high temperature	816:831	high temperature for the immobilized formula	816:859	The thermodynamics studies showed a thermal stability enhancement against high temperature for the immobilized formula.
29458101	3	42	from	5	559:559	arg1	pH					528:529	the optimal working pH	508:529	the optimal working pH for the free pectinase from 5	508:559	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	43	theme	optimum	601:607	arg1	temperature					621:631	the optimum operational temperature	597:631	the optimum operational temperature from 55°C to a higher temperature	597:665	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	44	theme	operational	609:619	arg1	temperature					621:631	the optimum operational temperature	597:631	the optimum operational temperature from 55°C to a higher temperature	597:665	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	45	theme	immobilization	475:488	arg1	process					490:496	The immobilization process	471:496	The immobilization process	471:496	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	1	46	theme	fungus	231:236	arg1	awamori					250:256	the fungus Aspergillus awamori KX943614	227:265	the fungus Aspergillus awamori KX943614	227:265	Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar.
29458101	4	47	theme	thermodynamics	746:759	arg1	studies					761:767	The thermodynamics studies	742:767	The thermodynamics studies	742:767	The thermodynamics studies showed a thermal stability enhancement against high temperature for the immobilized formula.
29458101	3	48	theme	higher	648:653	arg1	temperature					655:665	a higher temperature	646:665	a higher temperature	646:665	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	49	theme	temperature	621:631	arg1	range					574:578	a broader range	564:578	a broader range	564:578	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	4	50	theme	thermal	778:784	arg1	enhancement					796:806	a thermal stability enhancement	776:806	a thermal stability enhancement	776:806	The thermodynamics studies showed a thermal stability enhancement against high temperature for the immobilized formula.
29458101	1	51	theme	Aspergillus	238:248	arg1	awamori					250:256	the fungus Aspergillus awamori KX943614	227:265	the fungus Aspergillus awamori KX943614	227:265	Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar.
29458101	4	52	theme	stability	786:794	arg1	enhancement					796:806	a thermal stability enhancement	776:806	a thermal stability enhancement	776:806	The thermodynamics studies showed a thermal stability enhancement against high temperature for the immobilized formula.
29458101	6	53	theme	enzyme	1057:1062	arg1	enhancement					1074:1084	enzyme stability enhancement	1057:1084	enzyme stability enhancement	1057:1084	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	0	54	from	immobilization	26:39	arg1	beads					70:74	grafted alginate-agar gel beads	44:74	grafted alginate-agar gel beads	44:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	0	55	theme	pectinase	16:24	arg1	immobilization					26:39	pectinase immobilization	16:39	pectinase immobilization on grafted alginate-agar gel beads	16:74	Optimization of pectinase immobilization on grafted alginate-agar gel beads by 24 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization.
29458101	6	56	theme	free	1034:1037	arg1	energy					1039:1044	free energy	1034:1044	free energy	1034:1044	The thermodynamic studies proved that immobilization of pectinase made a remarkable increase in enthalpy and free energy because of enzyme stability enhancement.
29458101	3	57	from	55°C	638:641	arg1	pH4.5-5.5					583:591	pH4.5-5.5	583:591	pH4.5-5.5	583:591	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	57	from	55°C	638:641	arg1	range					574:578	a broader range	564:578	a broader range	564:578	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
29458101	3	57	from	55°C	638:641	arg1	temperature					621:631	the optimum operational temperature	597:631	the optimum operational temperature from 55°C to a higher temperature	597:665	The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH4.5-5.5 and the optimum operational temperature from 55°C to a higher temperature, of 60°C, which is favored to reduce the enzyme's microbial contamination.
31823833	4	0	theme	pH	660:661	arg1	levels					601:606	different levels	591:606	different levels of lactose-hydrolysis, sucrose addition and initial pH	591:661	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	8	1	from	differences	1190:1200	arg1	composition					1205:1215	composition	1205:1215	composition	1205:1215	The products showed significant differences in composition but all the values were in agreement with the literature.
31823833	1	2	theme	Dulce	166:170	arg1	DL					182:183	DL	182:183	DL	182:183	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	1	2	theme	Dulce	166:170	arg1	leche					175:179	Dulce de leche	166:179	Dulce de leche (DL)	166:184	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	9	3	theme	direct	1347:1352	arg1	relation					1354:1361	a direct relation	1345:1361	a direct relation	1345:1361	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	7	4	theme	dissolved	1011:1019	arg1	solids					1021:1026	dissolved solids	1011:1026	dissolved solids	1011:1026	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	1	5	theme	de	172:173	arg1	DL					182:183	DL	182:183	DL	182:183	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	1	5	theme	de	172:173	arg1	leche					175:179	Dulce de leche	166:179	Dulce de leche (DL)	166:184	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	9	6	theme	lower	1430:1434	arg1	coloration					1418:1427	coloration	1418:1427	coloration (lower luminosity)	1418:1446	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	6	theme	lower	1430:1434	arg1	luminosity					1436:1445	lower luminosity	1430:1445	lower luminosity	1430:1445	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	5	7	used	used	807:810	arg2	simulator					764:772	A process simulator	754:772	A process simulator with multi-monitoring system	754:801	A process simulator with multi-monitoring system was used to produce 15 DL.
31823833	9	8	theme	free	1480:1483	arg1	MR					1387:1388	MR	1387:1388	MR	1387:1388	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	6	9	theme	sucrose	933:939	arg1	concentration					941:953	sucrose concentration	933:953	sucrose concentration	933:953	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	6	9	theme	sucrose	933:939	arg1	factors					891:897	the three factors	881:897	the three factors: pH, lactose-hydrolysis level and sucrose concentration	881:953	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	7	10	theme	MR	1121:1122	arg1	indicators					1124:1133	MR indicators	1121:1133	MR indicators	1121:1133	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	6	11	theme	experimental	845:856	arg1	design					858:863	Box-Behnken 33 experimental design	830:863	Box-Behnken 33 experimental design	830:863	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	9	12	theme	more	1452:1455	arg1	formation					1457:1465	more formation	1452:1465	more formation of HMF	1452:1472	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	2	13	theme	reaction	272:279	arg1	products					286:293	Maillard reaction (MR) products	263:293	Maillard reaction (MR) products	263:293	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	7	14	dep	Lipids	956:961	arg1	total					1069:1073	total	1069:1073	total	1069:1073	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	7	14	dep	Lipids	956:961	arg1	free					1060:1063	free	1060:1063	free	1060:1063	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	10	15	theme	food	1598:1601	arg1	industries					1603:1612	the food industries	1594:1612	the food industries	1594:1612	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	7	16	theme	colorimetric	1029:1040	arg1	analysis					1042:1049	colorimetric analysis	1029:1049	colorimetric analysis	1029:1049	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	2	17	theme	Maillard	263:270	arg1	reaction					272:279	Maillard reaction	263:279	Maillard reaction (MR) products	263:293	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	2	17	theme	Maillard	263:270	arg1	MR					282:283	MR	282:283	MR	282:283	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	8	18	theme	significant	1178:1188	arg1	differences					1190:1200	significant differences	1178:1200	significant differences in composition	1178:1215	The products showed significant differences in composition but all the values were in agreement with the literature.
31823833	1	19	theme	leche	175:179	arg1	product					209:215	a traditional product	195:215	a traditional product from South America obtained by concentration	195:260	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	1	19	theme	leche	175:179	arg1	production					152:161	the production	148:161	the production	148:161	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	0	20	theme	lactose-hydrolyzed	54:71	arg1	leche					82:86	lactose-hydrolyzed Dulce de leche	54:86	lactose-hydrolyzed Dulce de leche	54:86	5-Hydroxymethylfurfural formation and color change in lactose-hydrolyzed Dulce de leche.
31823833	6	21	theme	Box-Behnken	830:840	arg1	design					858:863	Box-Behnken 33 experimental design	830:863	Box-Behnken 33 experimental design	830:863	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	5	22	theme	process	756:762	arg1	simulator					764:772	A process simulator	754:772	A process simulator with multi-monitoring system	754:801	A process simulator with multi-monitoring system was used to produce 15 DL.
31823833	3	23	theme	supply	424:429	arg1	demand					440:445	supply consumer demand	424:445	supply consumer demand	424:445	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	7	24	theme	physicochemical	1090:1104	arg1	attributes					1106:1115	the physicochemical attributes	1086:1115	the physicochemical attributes	1086:1115	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	2	25	from	formation	326:334	arg1	product					364:370	this product	359:370	this product	359:370	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	5	26	with	simulator	764:772	arg1	system					796:801	multi-monitoring system	779:801	multi-monitoring system	779:801	A process simulator with multi-monitoring system was used to produce 15 DL.
31823833	4	27	theme	sucrose	631:637	arg1	addition					639:646	sucrose addition	631:646	sucrose addition	631:646	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	0	28	theme	5-Hydroxymethylfurfural	0:22	arg1	formation					24:32	5-Hydroxymethylfurfural formation	0:32	5-Hydroxymethylfurfural formation	0:32	5-Hydroxymethylfurfural formation and color change in lactose-hydrolyzed Dulce de leche.
31823833	10	29	theme	present	1500:1506	arg1	study					1508:1512	The present study	1496:1512	The present study	1496:1512	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	3	30	theme	nutritional	527:537	arg1	aspects					539:545	some nutritional aspects	522:545	some nutritional aspects of the product	522:560	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	2	31	theme	flavor	349:354	arg1	formation					326:334	the formation	322:334	the formation of color and flavor in this product	322:370	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	6	32	theme	lactose-hydrolysis	904:921	arg1	factors					891:897	the three factors	881:897	the three factors: pH, lactose-hydrolysis level and sucrose concentration	881:953	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	6	32	theme	lactose-hydrolysis	904:921	arg1	level					923:927	lactose-hydrolysis level	904:927	lactose-hydrolysis level	904:927	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	9	33	from	increase	1406:1413	arg1	formation					1457:1465	more formation	1452:1465	more formation of HMF	1452:1472	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	33	from	increase	1406:1413	arg1	coloration					1418:1427	coloration	1418:1427	coloration (lower luminosity)	1418:1446	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	33	from	increase	1406:1413	arg1	luminosity					1436:1445	lower luminosity	1430:1445	lower luminosity	1430:1445	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	10	34	theme	traditional	1702:1712	arg1	product					1714:1720	the traditional product	1698:1720	the traditional product	1698:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	5	35	theme	multi-monitoring	779:794	arg1	system					796:801	multi-monitoring system	779:801	multi-monitoring system	779:801	A process simulator with multi-monitoring system was used to produce 15 DL.
31823833	4	36	theme	MR	685:686	arg1	development					670:680	the development	666:680	the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF)	666:751	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	7	37	theme	water	995:999	arg1	activity					1001:1008	water activity	995:1008	water activity	995:1008	Lipids, protein, ashes, carbohydrates, water activity, dissolved solids, colorimetric analysis and HMF (free and total) are among the physicochemical attributes and MR indicators analyzed in this work.
31823833	4	38	theme	5-hydroxymethylfurfural	723:745	arg1	determination					706:718	the determination	702:718	the determination of 5-hydroxymethylfurfural (HMF)	702:751	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	4	39	theme	initial	652:658	arg1	pH					660:661	initial pH	652:661	initial pH	652:661	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	10	40	theme	lactose-hydrolyzed	1560:1577	arg1	milk					1579:1582	lactose-hydrolyzed milk	1560:1582	lactose-hydrolyzed milk	1560:1582	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	9	41	theme	HMF	1470:1472	arg1	formation					1457:1465	more formation	1452:1465	more formation of HMF	1452:1472	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	41	theme	HMF	1470:1472	arg1	coloration					1418:1427	coloration	1418:1427	coloration (lower luminosity)	1418:1446	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	41	theme	HMF	1470:1472	arg1	luminosity					1436:1445	lower luminosity	1430:1445	lower luminosity	1430:1445	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	1	42	from	America	228:234	arg1	product					209:215	a traditional product	195:215	a traditional product from South America obtained by concentration	195:260	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	1	42	from	America	228:234	arg1	production					152:161	the production	148:161	the production	148:161	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	4	43	theme	levels	601:606	arg1	influence					578:586	the influence	574:586	the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF)	574:751	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	10	44	theme	closer	1688:1693	arg1	characteristics					1672:1686	nutritional and sensory characteristics	1648:1686	nutritional and sensory characteristics closer to the traditional product	1648:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	4	45	from	influence	578:586	arg1	development					670:680	the development	666:680	the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF)	666:751	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	3	46	theme	consumer	431:438	arg1	demand					440:445	supply consumer demand	424:445	supply consumer demand	424:445	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	4	47	theme	lactose-hydrolysis	611:628	arg1	levels					601:606	different levels	591:606	different levels of lactose-hydrolysis, sucrose addition and initial pH	591:661	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	9	48	theme	higher	1285:1290	arg1	levels					1292:1297	higher levels	1285:1297	higher levels of lactose hydrolysis	1285:1319	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	3	49	theme	product	554:560	arg1	flavor					483:488	the flavor	479:488	the flavor	479:488	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	3	49	theme	product	554:560	arg1	aspects					539:545	some nutritional aspects	522:545	some nutritional aspects of the product	522:560	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	3	49	theme	product	554:560	arg1	texture					505:511	texture	505:511	texture	505:511	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	3	49	theme	product	554:560	arg1	taste					498:502	taste	498:502	taste	498:502	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	3	49	theme	product	554:560	arg1	color					491:495	color	491:495	color	491:495	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	4	50	theme	different	591:599	arg1	levels					601:606	different levels	591:606	different levels of lactose-hydrolysis, sucrose addition and initial pH	591:661	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	0	51	theme	Dulce	73:77	arg1	leche					82:86	lactose-hydrolyzed Dulce de leche	54:86	lactose-hydrolyzed Dulce de leche	54:86	5-Hydroxymethylfurfural formation and color change in lactose-hydrolyzed Dulce de leche.
31823833	6	52	dep	factors	891:897	arg1	concentration					941:953	sucrose concentration	933:953	sucrose concentration	933:953	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	6	52	dep	factors	891:897	arg1	factors					891:897	the three factors	881:897	the three factors: pH, lactose-hydrolysis level and sucrose concentration	881:953	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	6	52	dep	factors	891:897	arg1	pH					900:901	pH	900:901	pH	900:901	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	6	52	dep	factors	891:897	arg1	level					923:927	lactose-hydrolysis level	904:927	lactose-hydrolysis level	904:927	Box-Behnken 33 experimental design was applied for the three factors: pH, lactose-hydrolysis level and sucrose concentration.
31823833	1	53	theme	Research	116:123	arg1	Communication					125:137	this Research Communication	111:137	this Research Communication	111:137	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	3	54	theme	Lactose-hydrolyzed	373:390	arg1	products					392:399	Lactose-hydrolyzed products	373:399	Lactose-hydrolyzed products	373:399	Lactose-hydrolyzed products have been developed to supply consumer demand, but this hydrolysis may affect the flavor, color, taste, texture and even some nutritional aspects of the product.
31823833	10	55	theme	free	1635:1638	arg1	DL					1640:1641	a lactose free DL	1625:1641	a lactose free DL with nutritional and sensory characteristics closer to the traditional product	1625:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	10	56	theme	sensory	1664:1670	arg1	characteristics					1672:1686	nutritional and sensory characteristics	1648:1686	nutritional and sensory characteristics closer to the traditional product	1648:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	10	57	theme	nutritional	1648:1658	arg1	characteristics					1672:1686	nutritional and sensory characteristics	1648:1686	nutritional and sensory characteristics closer to the traditional product	1648:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	9	58	theme	lactose	1302:1308	arg1	hydrolysis					1310:1319	lactose hydrolysis	1302:1319	lactose hydrolysis	1302:1319	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	59	theme	total	1489:1493	arg1	MR					1387:1388	MR	1387:1388	MR	1387:1388	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	60	theme	hydrolysis	1310:1319	arg1	levels					1292:1297	higher levels	1285:1297	higher levels of lactose hydrolysis	1285:1319	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	9	60	theme	hydrolysis	1310:1319	arg1	pH					1332:1333	higher pH	1325:1333	higher pH	1325:1333	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	1	61	theme	traditional	197:207	arg1	product					209:215	a traditional product	195:215	a traditional product from South America obtained by concentration	195:260	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	1	61	theme	traditional	197:207	arg1	production					152:161	the production	148:161	the production	148:161	The work described in this Research Communication concerns the production of Dulce de leche (DL), that is a traditional product from South America obtained by concentration.
31823833	10	62	theme	lactose	1627:1633	arg1	DL					1640:1641	a lactose free DL	1625:1641	a lactose free DL with nutritional and sensory characteristics closer to the traditional product	1625:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	8	63	with	agreement	1244:1252	arg1	literature					1263:1272	the literature	1259:1272	the literature	1259:1272	The products showed significant differences in composition but all the values were in agreement with the literature.
31823833	4	64	theme	addition	639:646	arg1	levels					601:606	different levels	591:606	different levels of lactose-hydrolysis, sucrose addition and initial pH	591:661	We studied the influence of different levels of lactose-hydrolysis, sucrose addition and initial pH on the development of MR, appraised by the determination of 5-hydroxymethylfurfural (HMF).
31823833	9	65	theme	MR	1387:1388	arg1	development					1372:1382	the development	1368:1382	the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total	1368:1493	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	10	66	theme	DL	1542:1543	arg1	spread					1545:1550	DL spread	1542:1550	DL spread made of lactose-hydrolyzed milk	1542:1582	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
31823833	9	67	theme	higher	1325:1330	arg1	pH					1332:1333	higher pH	1325:1333	higher pH	1325:1333	Moreover, higher levels of lactose hydrolysis and higher pH presented a direct relation with the development of MR, observed by an increase in coloration (lower luminosity) and more formation of HMF, both free and total.
31823833	0	68	theme	de	79:80	arg1	leche					82:86	lactose-hydrolyzed Dulce de leche	54:86	lactose-hydrolyzed Dulce de leche	54:86	5-Hydroxymethylfurfural formation and color change in lactose-hydrolyzed Dulce de leche.
31823833	2	69	theme	color	339:343	arg1	formation					326:334	the formation	322:334	the formation of color and flavor in this product	322:370	Maillard reaction (MR) products are mainly responsible for the formation of color and flavor in this product.
31823833	10	70	with	DL	1640:1641	arg1	characteristics					1672:1686	nutritional and sensory characteristics	1648:1686	nutritional and sensory characteristics closer to the traditional product	1648:1720	The present study expands the knowledge about DL spread made of lactose-hydrolyzed milk, allowing the food industries to produce a lactose free DL with nutritional and sensory characteristics closer to the traditional product.
29638048	0	0	theme	ethylene-vinyl	74:87	arg1	acetate					89:95	ethylene-vinyl acetate	74:95	ethylene-vinyl acetate	74:95	Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
29638048	4	1	theme	composites	761:770	arg1	manufacturing					744:756	the manufacturing	740:756	the manufacturing of composites	740:770	The ultrasound applied during the manufacturing of composites does not affect these characteristics.
29638048	11	2	dep	Biomater	1558:1565	arg1	400-407					1574:1580	400-407	1574:1580	400-407	1574:1580	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	1	3	theme	porous	522:527	arg1	scaffold					529:536	a porous scaffold	520:536	a porous scaffold	520:536	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	11	4	dep	Res	1541:1543	arg1	107B					1568:1571	107B	1568:1571	107B	1568:1571	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	11	4	dep	Res	1541:1543	arg1	B					1550:1550	Part B	1545:1550	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.	1526:1587	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	11	4	dep	Res	1541:1543	arg1	Biomater					1558:1565	Biomater	1558:1565	Biomater	1558:1565	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	11	4	dep	Res	1541:1543	arg1	2019					1583:1586	2019	1583:1586	2019	1583:1586	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	9	5	contain	have	1365:1368	arg2	behavior					1384:1391	improved cell behavior	1370:1391	improved cell behavior	1370:1391	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	5	contain	have	1365:1368	arg1	use					1307:1309	the use	1303:1309	the use of ultrasound in the production method (EVA)	1303:1354	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	7	6	theme	similar	1130:1136	arg1	reaction					1121:1128	inflammatory reaction	1108:1128	inflammatory reaction similar to PTFE	1108:1144	The data shows inflammatory reaction similar to PTFE.
29638048	9	7	theme	equivalent	1471:1480	arg1	response					1462:1469	tissue response	1455:1469	tissue response equivalent to the PTFE	1455:1492	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	2	8	theme	microscopy	557:566	arg1	images					568:573	Scanning electron microscopy images	539:573	Scanning electron microscopy images	539:573	Scanning electron microscopy images showed the influence of starch, reducing the diameter of pores.
29638048	9	9	theme	cell	1379:1382	arg1	behavior					1384:1391	improved cell behavior	1370:1391	improved cell behavior	1370:1391	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	1	10	theme	different	227:235	arg1	compositions					237:248	4 different compositions	225:248	4 different compositions	225:248	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	9	11	theme	improved	1370:1377	arg1	behavior					1384:1391	improved cell behavior	1370:1391	improved cell behavior	1370:1391	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	2	12	theme	electron	548:555	arg1	microscopy					557:566	Scanning electron microscopy	539:566	Scanning electron microscopy images	539:573	Scanning electron microscopy images showed the influence of starch, reducing the diameter of pores.
29638048	5	13	theme	PTFE	880:883	arg1	reaction					854:861	the tissue reaction	843:861	the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material	843:940	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	2	14	theme	Scanning	539:546	arg1	microscopy					557:566	Scanning electron microscopy	539:566	Scanning electron microscopy images	539:573	Scanning electron microscopy images showed the influence of starch, reducing the diameter of pores.
29638048	5	15	theme	materials	866:874	arg1	reaction					854:861	the tissue reaction	843:861	the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material	843:940	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	3	16	theme	starch	702:707	arg1	addition					690:697	the addition	686:697	the addition of starch	686:707	The number of open pores was also reduced with the addition of starch.
29638048	3	17	theme	pores	658:662	arg1	number					643:648	The number	639:648	The number of open pores	639:662	The number of open pores was also reduced with the addition of starch.
29638048	6	18	theme	microscopic	1025:1035	arg1	evaluation					1037:1046	microscopic evaluation	1025:1046	microscopic evaluation	1025:1046	After 7, 15, and 60 days of surgery, the materials were removed and processed for microscopic evaluation and counting of the inflammatory infiltrate.
29638048	1	19	from	processing	254:263	arg1	compositions					237:248	4 different compositions	225:248	4 different compositions	225:248	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	8	20	from	days	1191:1194	arg1	analysis					1176:1183	the quantitative analysis	1159:1183	the quantitative analysis at 60 days	1159:1194	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	1	21	theme	high	470:473	arg1	pressure					475:482	high pressure	470:482	high pressure without ultrasound	470:501	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	0	22	theme	subcutaneous	22:33	arg1	implants					35:42	subcutaneous implants	22:42	subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial	22:138	Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
29638048	3	23	theme	open	653:656	arg1	pores					658:662	open pores	653:662	open pores	653:662	The number of open pores was also reduced with the addition of starch.
29638048	8	24	theme	mononuclear	1242:1252	arg1	p < 0.05					1261:1268	p < 0.05	1261:1268	p < 0.05	1261:1268	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	8	24	theme	mononuclear	1242:1252	arg1	cells					1254:1258	mononuclear cells	1242:1258	mononuclear cells (p < 0.05)	1242:1269	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	10	25	dep	©	1495:1495	arg1	Inc.					1521:1524	Inc.	1521:1524	Inc.	1521:1524	© 2018 Wiley Periodicals, Inc.
29638048	1	26	theme	tissue	174:179	arg1	reaction					181:188	the tissue reaction	170:188	the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions	170:248	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	0	27	theme	future	112:117	arg1	use					119:121	future use	112:121	future use as a biomaterial	112:138	Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
29638048	8	28	theme	cells	1254:1258	arg1	quantity					1230:1237	less quantity	1225:1237	less quantity of mononuclear cells (p < 0.05)	1225:1269	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	5	29	theme	tissue	847:852	arg1	reaction					854:861	the tissue reaction	843:861	the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material	843:940	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	1	30	with	EVA	389:391	arg1	starch					402:407	15% starch	398:407	15% starch	398:407	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	31	theme	%	330:330	arg1	starch					332:337	15% starch	328:337	15% starch	328:337	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	9	32	theme	infiltration	1420:1431	arg1	reduction					1407:1415	the reduction	1403:1415	the reduction of infiltration over the period	1403:1447	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	33	theme	ultrasound	1314:1323	arg1	use					1307:1309	the use	1303:1309	the use of ultrasound in the production method (EVA)	1303:1354	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	34	theme	tissue	1455:1460	arg1	response					1462:1469	tissue response	1455:1469	tissue response equivalent to the PTFE	1455:1492	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	5	35	theme	proven	912:917	arg1	materials					866:874	materials	866:874	materials	866:874	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	5	35	theme	proven	912:917	arg1	material					933:940	biocompatible material	919:940	proven biocompatible material	912:940	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	1	36	theme	%	400:400	arg1	starch					402:407	15% starch	398:407	15% starch	398:407	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	37	theme	ethylene-vinyl	193:206	arg1	EVA					217:219	EVA	217:219	EVA	217:219	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	37	theme	ethylene-vinyl	193:206	arg1	acetate					208:214	ethylene-vinyl acetate	193:214	ethylene-vinyl acetate (EVA)	193:220	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	0	38	theme	new	49:51	arg1	material					53:60	a new material	47:60	a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial	47:138	Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
29638048	7	39	theme	inflammatory	1108:1119	arg1	reaction					1121:1128	inflammatory reaction	1108:1128	inflammatory reaction similar to PTFE	1108:1144	The data shows inflammatory reaction similar to PTFE.
29638048	5	40	theme	biocompatible	919:931	arg1	materials					866:874	materials	866:874	materials	866:874	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	5	40	theme	biocompatible	919:931	arg1	material					933:940	biocompatible material	919:940	proven biocompatible material	912:940	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	8	41	theme	quantitative	1163:1174	arg1	analysis					1176:1183	the quantitative analysis	1159:1183	the quantitative analysis at 60 days	1159:1194	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	1	42	theme	high	280:283	arg1	pressure					285:292	high pressure	280:292	high pressure	280:292	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	11	43	theme	Part	1545:1548	arg1	B					1550:1550	Part B	1545:1550	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.	1526:1587	J Biomed Mater Res Part B: Appl Biomater, 107B: 400-407, 2019.
29638048	9	44	from	use	1307:1309	arg1	method					1343:1348	the production method	1328:1348	the production method (EVA)	1328:1354	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	44	from	use	1307:1309	arg1	EVA					1351:1353	EVA	1351:1353	EVA	1351:1353	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	45	theme	production	1332:1341	arg1	method					1343:1348	the production method	1328:1348	the production method (EVA)	1328:1354	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	9	45	theme	production	1332:1341	arg1	EVA					1351:1353	EVA	1351:1353	EVA	1351:1353	Thus, the results suggest that the use of ultrasound in the production method (EVA) seems to have improved cell behavior regarding the reduction of infiltration over the period, with tissue response equivalent to the PTFE.
29638048	1	46	with	EVA	319:321	arg1	starch					332:337	15% starch	328:337	15% starch	328:337	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	47	theme	high	349:352	arg1	pressure					354:361	high pressure	349:361	high pressure	349:361	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	5	48	used	used	830:833	arg2	rats					820:823	Eighteen rats	811:823	Eighteen rats	811:823	Eighteen rats were used to test the tissue reaction of materials and PTFE (polytetrafluoroethylene), proven biocompatible material.
29638048	1	49	from	reaction	181:188	arg1	compositions					237:248	4 different compositions	225:248	4 different compositions	225:248	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	50	theme	future	506:511	arg1	use					513:515	future use	506:515	future use as a porous scaffold	506:536	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	51	theme	acetate	208:214	arg1	reaction					181:188	the tissue reaction	170:188	the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions	170:248	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	1	51	theme	acetate	208:214	arg1	processing					254:263	processing	254:263	processing	254:263	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	2	52	theme	starch	599:604	arg1	influence					586:594	the influence	582:594	the influence of starch	582:604	Scanning electron microscopy images showed the influence of starch, reducing the diameter of pores.
29638048	6	53	theme	infiltrate	1081:1090	arg1	counting					1052:1059	counting	1052:1059	counting	1052:1059	After 7, 15, and 60 days of surgery, the materials were removed and processed for microscopic evaluation and counting of the inflammatory infiltrate.
29638048	6	53	theme	infiltrate	1081:1090	arg1	evaluation					1037:1046	microscopic evaluation	1025:1046	microscopic evaluation	1025:1046	After 7, 15, and 60 days of surgery, the materials were removed and processed for microscopic evaluation and counting of the inflammatory infiltrate.
29638048	1	54	theme	high	419:422	arg1	pressure					424:431	high pressure	419:431	high pressure	419:431	This study aimed to evaluate the tissue reaction of ethylene-vinyl acetate (EVA) in 4 different compositions and processing: EVA foamed at high pressure with ultrasound (EVACU); EVA with 15% starch foamed at high pressure with ultrasound (EVAMCU); EVA with 15% starch foamed at high pressure without ultrasound and EVA foamed at high pressure without ultrasound as future use as a porous scaffold.
29638048	8	55	dep	EVACU	1201:1205	arg1	the					1197:1199	the	1197:1199	the	1197:1199	However, in the quantitative analysis at 60 days, the EVACU and EVAMCU showed less quantity of mononuclear cells (p < 0.05).
29638048	6	56	theme	inflammatory	1068:1079	arg1	infiltrate					1081:1090	the inflammatory infiltrate	1064:1090	the inflammatory infiltrate	1064:1090	After 7, 15, and 60 days of surgery, the materials were removed and processed for microscopic evaluation and counting of the inflammatory infiltrate.
29638048	2	57	theme	pores	632:636	arg1	diameter					620:627	the diameter	616:627	the diameter of pores	616:636	Scanning electron microscopy images showed the influence of starch, reducing the diameter of pores.
29638048	0	58	theme	material	53:60	arg1	implants					35:42	subcutaneous implants	22:42	subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial	22:138	Tissue reaction after subcutaneous implants of a new material composed of ethylene-vinyl acetate and starch for future use as a biomaterial.
29638048	6	59	theme	surgery	971:977	arg1	days					963:966	7, 15, and 60 days	949:966	7, 15, and 60 days of surgery	949:977	After 7, 15, and 60 days of surgery, the materials were removed and processed for microscopic evaluation and counting of the inflammatory infiltrate.
30143171	0	0	theme	enhanced	95:102	arg1	properties					130:139	enhanced mechanical and biological properties	95:139	enhanced mechanical and biological properties	95:139	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	4	1	from	changes	641:647	arg1	electrospinnability					694:712	electrospinnability	694:712	electrospinnability	694:712	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	1	from	changes	641:647	arg1	solutions					656:664	the solutions viscosity, conductivity and electrospinnability	652:712	the solutions viscosity, conductivity and electrospinnability	652:712	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	1	from	changes	641:647	arg1	conductivity					677:688	conductivity	677:688	conductivity	677:688	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	1	from	changes	641:647	arg1	viscosity					666:674	viscosity	666:674	viscosity	666:674	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	9	2	theme	PCL/NC	982:987	arg1	scaffolds					989:997	PCL/NC scaffolds	982:997	PCL/NC scaffolds	982:997	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	7	3	theme	%	827:827	arg1	NC					829:830	5-10 % NC	822:830	5-10 % NC made them electrospinnable	822:857	% were not electrospinnable, adding 5-10 % NC made them electrospinnable.
30143171	11	4	theme	cells	1241:1245	arg1	adhesion					1229:1236	adhesion	1229:1236	adhesion	1229:1236	In addition, proliferation and adhesion of cells was enhanced when NC was incorporated to nanofibers.
30143171	11	4	theme	cells	1241:1245	arg1	proliferation					1211:1223	proliferation	1211:1223	proliferation	1211:1223	In addition, proliferation and adhesion of cells was enhanced when NC was incorporated to nanofibers.
30143171	1	5	theme	PCL-based	288:296	arg1	scaffolds					298:306	PCL-based scaffolds	288:306	PCL-based scaffolds	288:306	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	0	6	theme	mechanical	104:113	arg1	properties					130:139	enhanced mechanical and biological properties	95:139	enhanced mechanical and biological properties	95:139	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	0	7	with	scaffolds	80:88	arg1	properties					130:139	enhanced mechanical and biological properties	95:139	enhanced mechanical and biological properties	95:139	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	1	8	theme	scaffolds	298:306	arg1	wettability					223:233	wettability	223:233	wettability	223:233	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	8	theme	scaffolds	298:306	arg1	properties					247:256	mechanical properties	236:256	mechanical properties	236:256	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	8	theme	scaffolds	298:306	arg1	compatibility					271:283	cellular compatibility	262:283	cellular compatibility	262:283	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	9	9	theme	remarkable	1006:1015	arg1	enhancement					1017:1027	remarkable enhancement	1006:1027	remarkable enhancement in both tensile strength and Young's modulus	1006:1072	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	10	10	theme	scaffolds	1171:1179	arg1	wettability					1156:1166	wettability	1156:1166	wettability of scaffolds after adding NC	1156:1195	Contact angle measurements revealed improvement in wettability of scaffolds after adding NC.
30143171	3	11	theme	constant	548:555	arg1	parameters					573:582	constant electrospinning parameters	548:582	constant electrospinning parameters	548:582	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	8	12	theme	cellular	903:910	arg1	wettability					887:897	wettability	887:897	wettability	887:897	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	12	theme	cellular	903:910	arg1	compatibility					912:924	cellular compatibility	903:924	cellular compatibility	903:924	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	12	theme	cellular	903:910	arg1	properties					875:884	The mechanical properties	860:884	The mechanical properties	860:884	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	12	theme	cellular	903:910	arg1	well					976:979	well	976:979	well	976:979	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	12	13	theme	tissue	1352:1357	arg1	applications					1371:1382	tissue engineering applications	1352:1382	tissue engineering applications	1352:1382	The results suggest PCL/NC as a proper scaffold for tissue engineering applications.
30143171	0	14	theme	biological	119:128	arg1	properties					130:139	enhanced mechanical and biological properties	95:139	enhanced mechanical and biological properties	95:139	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	3	15	theme	electrospinning	557:571	arg1	parameters					573:582	constant electrospinning parameters	548:582	constant electrospinning parameters	548:582	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	8	16	theme	electrospun	929:939	arg1	composites					948:957	electrospun PCL/NC composites	929:957	electrospun PCL/NC composites	929:957	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	5	17	with	solutions	735:743	arg1	concentration					750:762	concentration	750:762	concentration of 8 wt	750:770	Whiles the pure PCL solutions with concentration of 8 wt.
30143171	12	18	theme	engineering	1359:1369	arg1	applications					1371:1382	tissue engineering applications	1352:1382	tissue engineering applications	1352:1382	The results suggest PCL/NC as a proper scaffold for tissue engineering applications.
30143171	1	19	theme	facile	196:201	arg1	approach					203:210	a facile approach	194:210	a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds	194:306	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	19	theme	facile	196:201	arg1	composite					177:185	Fabricating polycaprolactone (PCL) composite	142:185	Fabricating polycaprolactone (PCL) composite	142:185	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	8	20	theme	composites	948:957	arg1	wettability					887:897	wettability	887:897	wettability	887:897	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	20	theme	composites	948:957	arg1	compatibility					912:924	cellular compatibility	903:924	cellular compatibility	903:924	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	20	theme	composites	948:957	arg1	properties					875:884	The mechanical properties	860:884	The mechanical properties	860:884	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	20	theme	composites	948:957	arg1	well					976:979	well	976:979	well	976:979	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	2	21	theme	PCL	390:392	arg1	matrix					394:399	PCL matrix	390:399	PCL matrix	390:399	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	0	22	theme	chitosan	33:40	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers	0:72	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	2	23	used	utilized	358:365	arg2	NC					350:351	NC	350:351	NC	350:351	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	2	23	used	utilized	358:365	arg2	chitosan					340:347	nanofibrillated chitosan	324:347	nanofibrillated chitosan (NC)	324:352	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	2	24	theme	nanofibrillated	324:338	arg1	NC					350:351	NC	350:351	NC	350:351	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	2	24	theme	nanofibrillated	324:338	arg1	chitosan					340:347	nanofibrillated chitosan	324:347	nanofibrillated chitosan (NC)	324:352	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	8	25	theme	PCL/NC	941:946	arg1	composites					948:957	electrospun PCL/NC composites	929:957	electrospun PCL/NC composites	929:957	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	0	26	theme	nanofibrillated	17:31	arg1	chitosan					33:40	nanofibrillated chitosan	17:40	nanofibrillated chitosan	17:40	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	4	27	theme	PCL	598:600	arg1	solutions					602:610	PCL solutions	598:610	PCL solutions	598:610	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	2	28	theme	fibrous	438:444	arg1	scaffolds					446:454	electrospun nanocomposite fibrous scaffolds	412:454	electrospun nanocomposite fibrous scaffolds	412:454	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	0	29	theme	electrospun	47:57	arg1	nanofibers					63:72	electrospun PCL nanofibers	47:72	electrospun PCL nanofibers	47:72	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	3	30	theme	NC	476:477	arg1	amounts					465:471	Various amounts	457:471	Various amounts of NC	457:477	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	3	30	theme	NC	476:477	arg1	NC					476:477	NC	476:477	NC	476:477	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	3	31	theme	Various	457:463	arg1	amounts					465:471	Various amounts	457:471	Various amounts of NC	457:477	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	3	31	theme	Various	457:463	arg1	NC					476:477	NC	476:477	NC	476:477	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	2	32	theme	nanocomposite	424:436	arg1	scaffolds					446:454	electrospun nanocomposite fibrous scaffolds	412:454	electrospun nanocomposite fibrous scaffolds	412:454	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	9	33	theme	Young	1058:1062	arg1	modulus					1066:1072	Young's modulus	1058:1072	Young's modulus	1058:1072	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	5	34	theme	8 wt	767:770	arg1	concentration					750:762	concentration	750:762	concentration of 8 wt	750:770	Whiles the pure PCL solutions with concentration of 8 wt.
30143171	9	35	theme	tensile	1037:1043	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	2	36	theme	electrospun	412:422	arg1	scaffolds					446:454	electrospun nanocomposite fibrous scaffolds	412:454	electrospun nanocomposite fibrous scaffolds	412:454	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	7	37	theme	5-10	822:825	arg1	%					827:827	%	827:827	%	827:827	% were not electrospinnable, adding 5-10 % NC made them electrospinnable.
30143171	0	38	theme	PCL	59:61	arg1	nanofibers					63:72	electrospun PCL nanofibers	47:72	electrospun PCL nanofibers	47:72	Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties.
30143171	5	39	theme	pure	726:729	arg1	solutions					735:743	the pure PCL solutions	722:743	the pure PCL solutions with concentration of 8 wt	722:770	Whiles the pure PCL solutions with concentration of 8 wt.
30143171	10	40	theme	angle	1113:1117	arg1	measurements					1119:1130	Contact angle measurements	1105:1130	Contact angle measurements	1105:1130	Contact angle measurements revealed improvement in wettability of scaffolds after adding NC.
30143171	4	41	dep	solutions	656:664	arg1	electrospinnability					694:712	electrospinnability	694:712	electrospinnability	694:712	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	41	dep	solutions	656:664	arg1	solutions					656:664	the solutions viscosity, conductivity and electrospinnability	652:712	the solutions viscosity, conductivity and electrospinnability	652:712	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	41	dep	solutions	656:664	arg1	conductivity					677:688	conductivity	677:688	conductivity	677:688	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	4	41	dep	solutions	656:664	arg1	viscosity					666:674	viscosity	666:674	viscosity	666:674	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	1	42	theme	Fabricating	142:152	arg1	polycaprolactone					154:169	Fabricating polycaprolactone	142:169	Fabricating polycaprolactone (PCL) composite	142:185	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	42	theme	Fabricating	142:152	arg1	PCL					172:174	PCL	172:174	PCL	172:174	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	2	43	from	phase	381:385	arg1	matrix					394:399	PCL matrix	390:399	PCL matrix	390:399	In this study, nanofibrillated chitosan (NC) was utilized as dispersing phase in PCL matrix to acquire electrospun nanocomposite fibrous scaffolds.
30143171	5	44	theme	PCL	731:733	arg1	solutions					735:743	the pure PCL solutions	722:743	the pure PCL solutions with concentration of 8 wt	722:770	Whiles the pure PCL solutions with concentration of 8 wt.
30143171	10	45	theme	Contact	1105:1111	arg1	measurements					1119:1130	Contact angle measurements	1105:1130	Contact angle measurements	1105:1130	Contact angle measurements revealed improvement in wettability of scaffolds after adding NC.
30143171	9	46	from	enhancement	1017:1027	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	1	47	theme	polycaprolactone	154:169	arg1	approach					203:210	a facile approach	194:210	a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds	194:306	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	47	theme	polycaprolactone	154:169	arg1	composite					177:185	Fabricating polycaprolactone (PCL) composite	142:185	Fabricating polycaprolactone (PCL) composite	142:185	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	1	48	theme	mechanical	236:245	arg1	properties					247:256	mechanical properties	236:256	mechanical properties	236:256	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
30143171	9	49	dep	showed	999:1004	arg1	compared					1074:1081	compared	1074:1081	showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold	999:1102	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	9	50	theme	neat	1086:1089	arg1	scaffold					1095:1102	neat PCL scaffold	1086:1102	neat PCL scaffold	1086:1102	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	8	51	theme	mechanical	864:873	arg1	wettability					887:897	wettability	887:897	wettability	887:897	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	51	theme	mechanical	864:873	arg1	well					976:979	well	976:979	well	976:979	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	51	theme	mechanical	864:873	arg1	properties					875:884	The mechanical properties	860:884	The mechanical properties	860:884	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	8	51	theme	mechanical	864:873	arg1	compatibility					912:924	cellular compatibility	903:924	cellular compatibility	903:924	The mechanical properties, wettability and cellular compatibility of electrospun PCL/NC composites were improved as well.
30143171	3	52	theme	PCL	493:495	arg1	solutions					497:505	PCL solutions	493:505	PCL solutions	493:505	Various amounts of NC were added to PCL solutions and the solutions were electrospun under constant electrospinning parameters.
30143171	12	53	theme	proper	1332:1337	arg1	scaffold					1339:1346	a proper scaffold	1330:1346	a proper scaffold for tissue engineering applications	1330:1382	The results suggest PCL/NC as a proper scaffold for tissue engineering applications.
30143171	12	53	theme	proper	1332:1337	arg1	PCL/NC					1320:1325	PCL/NC	1320:1325	PCL/NC	1320:1325	The results suggest PCL/NC as a proper scaffold for tissue engineering applications.
30143171	9	54	theme	PCL	1091:1093	arg1	scaffold					1095:1102	neat PCL scaffold	1086:1102	neat PCL scaffold	1086:1102	PCL/NC scaffolds showed remarkable enhancement in both tensile strength and Young's modulus compared to neat PCL scaffold.
30143171	10	55	from	improvement	1141:1151	arg1	wettability					1156:1166	wettability	1156:1166	wettability of scaffolds after adding NC	1156:1195	Contact angle measurements revealed improvement in wettability of scaffolds after adding NC.
30143171	4	56	theme	notable	633:639	arg1	changes					641:647	notable changes	633:647	notable changes in the solutions viscosity, conductivity and electrospinnability	633:712	Adding NC to PCL solutions was accompanied with notable changes in the solutions viscosity, conductivity and electrospinnability.
30143171	1	57	theme	cellular	262:269	arg1	compatibility					271:283	cellular compatibility	262:283	cellular compatibility	262:283	Fabricating polycaprolactone (PCL) composite can be a facile approach to improve wettability, mechanical properties and cellular compatibility of PCL-based scaffolds.
31266859	13	0	dep	both	1790:1793	arg1	level					1802:1806	the level	1798:1806	the level of the uptake	1798:1820	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	15	1	theme	higher	2077:2082	arg1	production					2094:2103	higher cellulase production	2077:2103	higher cellulase production	2077:2103	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	9	2	theme	enzymes	1286:1292	arg1	production					1272:1281	the production	1268:1281	the production of enzymes for polysaccharide degradation	1268:1323	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	7	3	dep	identified	855:864	arg1	both					866:869	both	866:869	both	866:869	Crosstalk and competitive inhibition could be identified both during uptake by cellodextrin transporters and intracellularly.
31266859	7	3	dep	identified	855:864	arg1	intracellularly					918:932	intracellularly	918:932	intracellularly	918:932	Crosstalk and competitive inhibition could be identified both during uptake by cellodextrin transporters and intracellularly.
31266859	9	4	theme	polysaccharide	1298:1311	arg1	degradation					1313:1323	polysaccharide degradation	1298:1323	polysaccharide degradation	1298:1323	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	5	5	theme	crosstalk	502:510	arg1	evidence					490:497	evidence	490:497	evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression	490:684	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	11	6	theme	perception	1558:1567	arg1	pathways					1569:1576	two seemingly unrelated perception pathways	1534:1576	two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan	1534:1633	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	9	7	theme	fungi	1103:1107	arg1	networks					1073:1080	the regulatory networks	1058:1080	the regulatory networks of lignocellulolytic fungi	1058:1107	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	8	8	dep	the	948:950	arg1	overlap					952:958	overlap	952:958	overlap	952:958	Importantly, the overlap is independent of CRE-1-mediated catabolite repression.
31266859	7	9	theme	cellodextrin	888:899	arg1	transporters					901:912	cellodextrin transporters	888:912	cellodextrin transporters	888:912	Crosstalk and competitive inhibition could be identified both during uptake by cellodextrin transporters and intracellularly.
31266859	5	10	theme	cellulase	665:673	arg1	expression					675:684	cellulase expression	665:684	cellulase expression	665:684	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	9	11	theme	regulatory	1062:1071	arg1	networks					1073:1080	the regulatory networks	1058:1080	the regulatory networks of lignocellulolytic fungi	1058:1107	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	9	12	theme	signaling	1350:1358	arg1	networks					1360:1367	complex signaling networks	1342:1367	complex signaling networks	1342:1367	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	10	13	theme	single	1442:1447	arg1	polysaccharides					1449:1463	single polysaccharides	1442:1463	single polysaccharides	1442:1463	Previously, these networks were studied in response to simple sugars or single polysaccharides.
31266859	12	14	theme	signaling	1744:1752	arg1	molecules					1754:1762	signaling molecules	1744:1762	signaling molecules	1744:1762	We identified a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules.
31266859	13	15	theme	main	1859:1862	arg1	CLR-2					1890:1894	the main transcriptional regulator CLR-2	1855:1894	the main transcriptional regulator CLR-2	1855:1894	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	13	16	theme	regulator	1880:1888	arg1	CLR-2					1890:1894	the main transcriptional regulator CLR-2	1855:1894	the main transcriptional regulator CLR-2	1855:1894	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	2	17	theme	soluble	215:221	arg1	products					235:242	soluble degradation products	215:242	soluble degradation products of plant cell wall components	215:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	12	18	theme	degradation	1713:1723	arg1	products					1725:1732	the respective degradation products	1698:1732	the respective degradation products acting as signaling molecules	1698:1762	We identified a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules.
31266859	11	19	theme	gluco	1622:1626	arg1	mannan					1628:1633	the hemicellulose (gluco)mannan	1603:1633	the hemicellulose (gluco)mannan	1603:1633	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	2	20	theme	wall	258:261	arg1	components					263:272	plant cell wall components	247:272	plant cell wall components	247:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	9	21	theme	plant	1200:1204	arg1	depolymerization					1214:1229	efficient plant biomass depolymerization	1190:1229	efficient plant biomass depolymerization	1190:1229	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	12	22	theme	unknown	1659:1665	arg1	inhibition					1679:1688	a so far unknown competitive inhibition	1650:1688	a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules	1650:1762	We identified a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules.
31266859	3	23	theme	perception	339:348	arg1	pathways					350:357	Individual perception pathways	328:357	Individual perception pathways	328:357	Individual perception pathways have been well described.
31266859	11	24	theme	hemicellulose	1607:1619	arg1	mannan					1628:1633	the hemicellulose (gluco)mannan	1603:1633	the hemicellulose (gluco)mannan	1603:1633	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	2	25	theme	plant	247:251	arg1	components					263:272	plant cell wall components	247:272	plant cell wall components	247:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	9	26	theme	rational	1136:1143	arg1	optimization					1145:1156	the rational optimization	1132:1156	the rational optimization of fungal enzyme production for efficient plant biomass depolymerization	1132:1229	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	14	27	theme	perception	1974:1983	arg1	pathways					1985:1992	perception pathways	1974:1992	perception pathways	1974:1992	Our findings provide novel insights into the molecular communication between perception pathways.
31266859	0	28	theme	Production	87:96	arg1	Inhibition					63:72	Inhibition	63:72	Inhibition of Cellulase Production in Several Filamentous Fungi	63:125	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	6	29	theme	functional	699:708	arg1	tools					719:723	the functional genomics tools	695:723	the functional genomics tools available for Neurospora crassa	695:755	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	6	30	theme	Neurospora	739:748	arg1	crassa					750:755	Neurospora crassa	739:755	Neurospora crassa	739:755	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	0	31	theme	Several	101:107	arg1	Fungi					121:125	Several Filamentous Fungi	101:125	Several Filamentous Fungi	101:125	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	13	32	theme	uptake	1815:1820	arg1	level					1802:1806	the level	1798:1806	the level of the uptake	1798:1820	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	6	33	theme	available	725:733	arg1	tools					719:723	the functional genomics tools	695:723	the functional genomics tools available for Neurospora crassa	695:755	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	5	34	theme	several	606:612	arg1	fungi					626:630	several filamentous fungi	606:630	several filamentous fungi	606:630	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	6	35	theme	genomics	710:717	arg1	tools					719:723	the functional genomics tools	695:723	the functional genomics tools available for Neurospora crassa	695:755	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	0	36	theme	Cellulose	13:21	arg1	Pathways					45:52	Cellulose and Mannan Perception Pathways	13:52	Cellulose and Mannan Perception Pathways	13:52	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	14	37	theme	molecular	1942:1950	arg1	communication					1952:1964	the molecular communication	1938:1964	the molecular communication between perception pathways	1938:1992	Our findings provide novel insights into the molecular communication between perception pathways.
31266859	10	38	theme	simple	1425:1430	arg1	sugars					1432:1437	simple sugars	1425:1437	simple sugars	1425:1437	Previously, these networks were studied in response to simple sugars or single polysaccharides.
31266859	0	39	theme	Mannan	27:32	arg1	Pathways					45:52	Cellulose and Mannan Perception Pathways	13:52	Cellulose and Mannan Perception Pathways	13:52	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	8	40	theme	catabolite	993:1002	arg1	repression					1004:1013	CRE-1-mediated catabolite repression	978:1013	CRE-1-mediated catabolite repression	978:1013	Importantly, the overlap is independent of CRE-1-mediated catabolite repression.
31266859	9	41	theme	novel	1038:1042	arg1	insights					1044:1051	novel insights	1038:1051	novel insights into the regulatory networks of lignocellulolytic fungi	1038:1107	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	6	42	theme	molecular	792:800	arg1	level					802:806	the molecular level	788:806	the molecular level	788:806	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	0	43	theme	Pathways	45:52	arg1	Crosstalk					0:8	Crosstalk	0:8	Crosstalk of Cellulose and Mannan Perception Pathways	0:52	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	14	44	theme	novel	1918:1922	arg1	insights					1924:1931	novel insights	1918:1931	novel insights into the molecular communication between perception pathways	1918:1992	Our findings provide novel insights into the molecular communication between perception pathways.
31266859	15	45	theme	insensitivity	2139:2151	arg1	engineering					2117:2127	the engineering	2113:2127	the engineering of mannan insensitivity	2113:2151	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	5	46	theme	present	465:471	arg1	work					473:476	the present work	461:476	the present work	461:476	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	6	47	used	used	690:693	arg2	We					687:688	We	687:688	We	687:688	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	11	48	theme	first	1491:1495	arg1	time					1497:1500	the first time	1487:1500	the first time	1487:1500	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	5	49	theme	expression	675:684	arg1	inhibition					651:660	the inhibition	647:660	the inhibition of cellulase expression	647:684	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	2	50	theme	substrate	292:300	arg1	composition					302:312	the substrate composition	288:312	the substrate composition	288:312	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	13	51	dep	detected	1781:1788	arg1	intracellularly					1826:1840	intracellularly	1826:1840	intracellularly	1826:1840	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	13	51	dep	detected	1781:1788	arg1	both					1790:1793	both	1790:1793	both	1790:1793	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	15	52	theme	strains	2065:2071	arg1	improvement					2039:2049	the improvement	2035:2049	the improvement of industrial strains	2035:2071	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	5	53	theme	perception	524:533	arg1	pathways					535:542	the perception pathways	520:542	the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression	520:684	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	9	54	theme	complex	1342:1348	arg1	networks					1360:1367	complex signaling networks	1342:1367	complex signaling networks	1342:1367	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	12	55	theme	respective	1702:1711	arg1	products					1725:1732	the respective degradation products	1698:1732	the respective degradation products acting as signaling molecules	1698:1762	We identified a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules.
31266859	0	56	from	Inhibition	63:72	arg1	Fungi					121:125	Several Filamentous Fungi	101:125	Several Filamentous Fungi	101:125	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	9	57	theme	lignocellulolytic	1085:1101	arg1	fungi					1103:1107	lignocellulolytic fungi	1085:1107	lignocellulolytic fungi	1085:1107	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	2	58	theme	degradation	223:233	arg1	products					235:242	soluble degradation products	215:242	soluble degradation products of plant cell wall components	215:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	5	59	theme	hemicellulose	566:578	arg1	mannan					580:585	the hemicellulose mannan	562:585	the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression	562:684	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	11	60	dep	pathways	1569:1576	arg1	those					1579:1583	those	1579:1583	those	1579:1583	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	2	61	theme	components	263:272	arg1	products					235:242	soluble degradation products	215:242	soluble degradation products of plant cell wall components	215:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	13	62	theme	transcriptional	1864:1878	arg1	CLR-2					1890:1894	the main transcriptional regulator CLR-2	1855:1894	the main transcriptional regulator CLR-2	1855:1894	Competition was detected both at the level of the uptake and intracellularly, upstream of the main transcriptional regulator CLR-2.
31266859	12	63	theme	competitive	1667:1677	arg1	inhibition					1679:1688	a so far unknown competitive inhibition	1650:1688	a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules	1650:1762	We identified a so far unknown competitive inhibition between the respective degradation products acting as signaling molecules.
31266859	2	64	theme	cell	253:256	arg1	components					263:272	plant cell wall components	247:272	plant cell wall components	247:272	Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on.
31266859	15	65	theme	industrial	2054:2063	arg1	strains					2065:2071	industrial strains	2054:2071	industrial strains	2054:2071	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	0	66	theme	Cellulase	77:85	arg1	Production					87:96	Cellulase Production	77:96	Cellulase Production	77:96	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	3	67	theme	Individual	328:337	arg1	pathways					350:357	Individual perception pathways	328:357	Individual perception pathways	328:357	Individual perception pathways have been well described.
31266859	15	68	theme	mannan	2132:2137	arg1	insensitivity					2139:2151	mannan insensitivity	2132:2151	mannan insensitivity	2132:2151	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	9	69	theme	fungal	1161:1166	arg1	production					1175:1184	fungal enzyme production	1161:1184	fungal enzyme production	1161:1184	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	0	70	theme	Filamentous	109:119	arg1	Fungi					121:125	Several Filamentous Fungi	101:125	Several Filamentous Fungi	101:125	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	11	71	theme	unrelated	1548:1556	arg1	pathways					1569:1576	two seemingly unrelated perception pathways	1534:1576	two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan	1534:1633	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	15	72	theme	cellulase	2084:2092	arg1	production					2094:2103	higher cellulase production	2077:2103	higher cellulase production	2077:2103	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31266859	9	73	theme	efficient	1190:1198	arg1	depolymerization					1214:1229	efficient plant biomass depolymerization	1190:1229	efficient plant biomass depolymerization	1190:1229	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	5	74	theme	filamentous	614:624	arg1	fungi					626:630	several filamentous fungi	606:630	several filamentous fungi	606:630	In the present work, we provide evidence of crosstalk between the perception pathways for cellulose and the hemicellulose mannan being conserved in several filamentous fungi and leading to the inhibition of cellulase expression.
31266859	9	75	theme	biomass	1206:1212	arg1	depolymerization					1214:1229	efficient plant biomass depolymerization	1190:1229	efficient plant biomass depolymerization	1190:1229	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	0	76	theme	Perception	34:43	arg1	Pathways					45:52	Cellulose and Mannan Perception Pathways	13:52	Cellulose and Mannan Perception Pathways	13:52	Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi.
31266859	9	77	theme	enzyme	1168:1173	arg1	production					1175:1184	fungal enzyme production	1161:1184	fungal enzyme production	1161:1184	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	7	78	theme	competitive	823:833	arg1	inhibition					835:844	competitive inhibition	823:844	competitive inhibition	823:844	Crosstalk and competitive inhibition could be identified both during uptake by cellodextrin transporters and intracellularly.
31266859	11	79	theme	molecular	1506:1514	arg1	interplay					1516:1524	the molecular interplay	1502:1524	the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan	1502:1633	Here, we tackled for the first time the molecular interplay between two seemingly unrelated perception pathways: those for cellulose and the hemicellulose (gluco)mannan.
31266859	8	80	theme	CRE-1-mediated	978:991	arg1	repression					1004:1013	CRE-1-mediated catabolite repression	978:1013	CRE-1-mediated catabolite repression	978:1013	Importantly, the overlap is independent of CRE-1-mediated catabolite repression.
31266859	8	81	theme	repression	1004:1013	arg1	independent					963:973	independent	963:973	independent	963:973	Importantly, the overlap is independent of CRE-1-mediated catabolite repression.
31266859	7	82	dep	both	866:869	arg1	uptake					878:883	uptake	878:883	uptake by cellodextrin transporters	878:912	Crosstalk and competitive inhibition could be identified both during uptake by cellodextrin transporters and intracellularly.
31266859	9	83	theme	production	1175:1184	arg1	optimization					1145:1156	the rational optimization	1132:1156	the rational optimization of fungal enzyme production for efficient plant biomass depolymerization	1132:1229	These results provide novel insights into the regulatory networks of lignocellulolytic fungi and will contribute to the rational optimization of fungal enzyme production for efficient plant biomass depolymerization and utilization.IMPORTANCE In fungi, the production of enzymes for polysaccharide degradation is controlled by complex signaling networks.
31266859	6	84	dep	this	772:775	arg1	overlap					777:783	overlap	777:783	overlap at the molecular level	777:806	We used the functional genomics tools available for Neurospora crassa to investigate this overlap at the molecular level.
31266859	15	85	theme	possible	2014:2021	arg1	targets					2023:2029	possible targets	2014:2029	possible targets for the improvement of industrial strains for higher cellulase production	2014:2103	Also, they present possible targets for the improvement of industrial strains for higher cellulase production through the engineering of mannan insensitivity.
31864180	5	0	theme	short-branch	672:683	arg1	CAP					685:687	The very short-branch CAP	663:687	The very short-branch CAP	663:687	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	0	1	theme	branching	88:96	arg1	enzymes					98:104	glycogen branching enzymes	79:104	glycogen branching enzymes having different branching properties	79:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	8	2	theme	complicated	1276:1286	arg1	manner					1288:1293	complicated manner	1276:1293	complicated manner	1276:1293	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	3	3	theme	water	534:538	arg1	solubility					540:549	water solubility	534:549	water solubility	534:549	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	6	4	theme	larger	913:918	arg1	diameter					933:940	1.8-fold larger hydrodynamic diameter	904:940	1.8-fold larger hydrodynamic diameter	904:940	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	0	5	theme	different	113:121	arg1	properties					133:142	different branching properties	113:142	different branching properties	113:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	7	6	theme	12-day	1026:1031	arg1	feeding					1033:1039	12-day feeding	1026:1039	12-day feeding of the CAPs	1026:1051	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	3	7	from	effects	571:577	arg1	gut-microbiota					587:600	mice gut-microbiota	582:600	mice gut-microbiota	582:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	7	8	dep	clear	1092:1096	arg1	positive					1098:1105	positive	1098:1105	positive	1098:1105	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	6	9	theme	short	956:960	arg1	one					962:964	the very short one	947:964	the very short one	947:964	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	3	10	from	solubility	540:549	arg1	gut-microbiota					587:600	mice gut-microbiota	582:600	mice gut-microbiota	582:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	8	11	theme	CAP	1233:1235	arg1	structure					1237:1245	the CAP structure	1229:1245	the CAP structure	1229:1245	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	3	12	with	correlation	494:504	arg1	digestibility					552:564	digestibility	552:564	digestibility	552:564	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	3	12	with	correlation	494:504	arg1	effects					571:577	effects	571:577	effects on mice gut-microbiota	571:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	3	12	with	correlation	494:504	arg1	solubility					540:549	water solubility	534:549	water solubility	534:549	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	3	12	with	correlation	494:504	arg1	diameter					524:531	hydrodynamic diameter	511:531	hydrodynamic diameter	511:531	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	0	13	theme	branching	123:131	arg1	properties					133:142	different branching properties	113:142	different branching properties	113:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	5	14	theme	small	814:818	arg1	condition					840:848	small intestine-mimicking condition	814:848	small intestine-mimicking condition	814:848	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	5	15	dep	more	699:702	arg1	water-solubility					718:733	water-solubility	718:733	water-solubility	718:733	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	5	15	dep	more	699:702	arg1	efficiency					771:780	3.5-fold lower α-amylase catalytic efficiency	736:780	3.5-fold lower α-amylase catalytic efficiency	736:780	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	5	15	dep	more	699:702	arg1	%					789:789	27%	787:789	27%	787:789	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	8	16	theme	single	1213:1218	arg1	GBE					1220:1222	the single GBE	1209:1222	the single GBE	1209:1222	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	1	17	theme	different	202:210	arg1	characteristics					222:236	different branching characteristics	202:236	different branching characteristics	202:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	0	18	theme	Structural	0:9	arg1	characteristics					26:40	Structural and functional characteristics	0:40	Structural and functional characteristics of clustered amylopectin	0:65	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	1	19	theme	branching	212:220	arg1	characteristics					222:236	different branching characteristics	202:236	different branching characteristics	202:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	3	20	from	diameter	524:531	arg1	gut-microbiota					587:600	mice gut-microbiota	582:600	mice gut-microbiota	582:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	6	21	contain	had	900:902	arg1	CAP					896:898	medium branch CAP	882:898	medium branch CAP	882:898	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	6	21	contain	had	900:902	arg2	diameter					933:940	1.8-fold larger hydrodynamic diameter	904:940	1.8-fold larger hydrodynamic diameter	904:940	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	0	22	theme	functional	15:24	arg1	characteristics					26:40	Structural and functional characteristics	0:40	Structural and functional characteristics of clustered amylopectin	0:65	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	1	23	theme	functional	306:315	arg1	properties					317:326	functional properties	306:326	functional properties	306:326	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	5	24	theme	lower	745:749	arg1	efficiency					771:780	3.5-fold lower α-amylase catalytic efficiency	736:780	3.5-fold lower α-amylase catalytic efficiency	736:780	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	3	25	theme	mice	582:585	arg1	gut-microbiota					587:600	mice gut-microbiota	582:600	mice gut-microbiota	582:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	3	26	theme	profound	485:492	arg1	correlation					494:504	profound correlation	485:504	profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota	485:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	0	27	theme	clustered	45:53	arg1	amylopectin					55:65	clustered amylopectin	45:65	clustered amylopectin	45:65	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	5	28	theme	α-amylase	751:759	arg1	efficiency					771:780	3.5-fold lower α-amylase catalytic efficiency	736:780	3.5-fold lower α-amylase catalytic efficiency	736:780	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	3	29	theme	Branch	458:463	arg1	distribution					465:476	Branch distribution	458:476	Branch distribution	458:476	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	5	30	theme	intestine-mimicking	820:838	arg1	condition					840:848	small intestine-mimicking condition	814:848	small intestine-mimicking condition	814:848	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	6	31	dep	larger	913:918	arg1	hydrodynamic					920:931	hydrodynamic	920:931	hydrodynamic	920:931	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	5	32	theme	catalytic	761:769	arg1	efficiency					771:780	3.5-fold lower α-amylase catalytic efficiency	736:780	3.5-fold lower α-amylase catalytic efficiency	736:780	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	1	33	contain	having	195:200	arg1	enzymes					180:186	Three bacterial glycogen branching enzymes	145:186	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics	145:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	33	contain	having	195:200	arg2	characteristics					222:236	different branching characteristics	202:236	different branching characteristics	202:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	33	contain	having	195:200	arg1	GBEs					189:192	GBEs	189:192	GBEs	189:192	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	34	used	used	243:246	arg2	GBEs					189:192	GBEs	189:192	GBEs	189:192	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	34	used	used	243:246	arg2	enzymes					180:186	Three bacterial glycogen branching enzymes	145:186	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics	145:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	7	35	theme	chain	1074:1078	arg1	CAP					1080:1082	only the medium chain CAP	1058:1082	only the medium chain CAP	1058:1082	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	1	36	theme	CAPs	331:334	arg1	structure					292:300	structure	292:300	structure	292:300	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	36	theme	CAPs	331:334	arg1	properties					317:326	functional properties	306:326	functional properties	306:326	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	3	37	theme	hydrodynamic	511:522	arg1	diameter					524:531	hydrodynamic diameter	511:531	hydrodynamic diameter	511:531	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31864180	0	38	theme	amylopectin	55:65	arg1	characteristics					26:40	Structural and functional characteristics	0:40	Structural and functional characteristics of clustered amylopectin	0:65	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	8	39	dep	in	1255:1256	arg1	vivo					1258:1261	vivo	1258:1261	vivo	1258:1261	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	5	40	from	digestibility	797:809	arg1	condition					840:848	small intestine-mimicking condition	814:848	small intestine-mimicking condition	814:848	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	0	41	contain	having	106:111	arg2	properties					133:142	different branching properties	113:142	different branching properties	113:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	0	41	contain	having	106:111	arg1	enzymes					98:104	glycogen branching enzymes	79:104	glycogen branching enzymes having different branching properties	79:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	2	42	theme	Branch	363:368	arg1	distributions					370:382	Branch distributions	363:382	Branch distributions of three CAPs	363:396	Branch distributions of three CAPs varied from very short (DPn = 6.65) to medium (DPn = 14.1).
31864180	7	43	theme	Mice	967:970	arg1	composition					987:997	Mice gut-microbiota composition	967:997	Mice gut-microbiota composition statistically	967:1011	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	8	44	theme	digestible	1159:1168	arg1	starch					1170:1175	slowly digestible starch	1152:1175	slowly digestible starch	1152:1175	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	7	45	theme	medium	1067:1072	arg1	CAP					1080:1082	only the medium chain CAP	1058:1082	only the medium chain CAP	1058:1082	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	1	46	theme	bacterial	151:159	arg1	enzymes					180:186	Three bacterial glycogen branching enzymes	145:186	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics	145:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	46	theme	bacterial	151:159	arg1	GBEs					189:192	GBEs	189:192	GBEs	189:192	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	7	47	theme	gut-microbiota	972:985	arg1	composition					987:997	Mice gut-microbiota composition	967:997	Mice gut-microbiota composition statistically	967:1011	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	7	48	theme	clear	1092:1096	arg1	changes					1107:1113	clear positive changes	1092:1113	clear positive changes	1092:1113	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	6	49	theme	branch	889:894	arg1	CAP					896:898	medium branch CAP	882:898	medium branch CAP	882:898	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	1	50	theme	glycogen	161:168	arg1	enzymes					180:186	Three bacterial glycogen branching enzymes	145:186	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics	145:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	50	theme	glycogen	161:168	arg1	GBEs					189:192	GBEs	189:192	GBEs	189:192	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	51	theme	clustered	259:267	arg1	amylopectin					269:279	clustered amylopectin	259:279	clustered amylopectin (CAP)	259:285	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	51	theme	clustered	259:267	arg1	CAP					282:284	CAP	282:284	CAP	282:284	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	0	52	theme	glycogen	79:86	arg1	enzymes					98:104	glycogen branching enzymes	79:104	glycogen branching enzymes having different branching properties	79:142	Structural and functional characteristics of clustered amylopectin produced by glycogen branching enzymes having different branching properties.
31864180	6	53	theme	medium	882:887	arg1	CAP					896:898	medium branch CAP	882:898	medium branch CAP	882:898	Intriguingly, medium branch CAP had 1.8-fold larger hydrodynamic diameter than the very short one.
31864180	1	54	theme	branching	170:178	arg1	enzymes					180:186	Three bacterial glycogen branching enzymes	145:186	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics	145:236	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	1	54	theme	branching	170:178	arg1	GBEs					189:192	GBEs	189:192	GBEs	189:192	Three bacterial glycogen branching enzymes (GBEs) having different branching characteristics were used to produce clustered amylopectin (CAP), and structure and functional properties of CAPs were intensively analyzed.
31864180	5	55	theme	lower	791:795	arg1	digestibility					797:809	more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility	699:809	more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition	699:848	The very short-branch CAP exhibited more than 100-fold water-solubility, 3.5-fold lower α-amylase catalytic efficiency, and 27% lower digestibility in small intestine-mimicking condition than amylopectin.
31864180	8	56	theme	in	1255:1256	arg1	functions					1263:1271	in vivo functions	1255:1271	in vivo functions	1255:1271	Consequently, slowly digestible starch was successfully synthesized by the single GBE, but the CAP structure affects in vivo functions in complicated manner.
31864180	7	57	theme	CAPs	1048:1051	arg1	feeding					1033:1039	12-day feeding	1026:1039	12-day feeding of the CAPs	1026:1051	Mice gut-microbiota composition statistically varied after 12-day feeding of the CAPs, but only the medium chain CAP brought clear positive changes on the gut-microbiota.
31864180	2	58	theme	CAPs	393:396	arg1	distributions					370:382	Branch distributions	363:382	Branch distributions of three CAPs	363:396	Branch distributions of three CAPs varied from very short (DPn = 6.65) to medium (DPn = 14.1).
31864180	3	59	from	digestibility	552:564	arg1	gut-microbiota					587:600	mice gut-microbiota	582:600	mice gut-microbiota	582:600	Branch distribution showed profound correlation with hydrodynamic diameter, water solubility, digestibility, and effects on mice gut-microbiota.
31022490	7	0	theme	lines	1160:1164	arg1	inhibition					1126:1135	significant inhibition	1114:1135	significant inhibition of cancer cell (HepG2) lines	1114:1164	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	4	1	with	film	659:662	arg1	286.91 °C					676:684	3% SNC 286.91 °C	669:684	3% SNC 286.91 °C	669:684	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	4	1	with	film	659:662	arg1	film					690:693	film	690:693	film	690:693	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	3	2	theme	nanocrystals	460:471	arg1	incorporation					436:448	The incorporation	432:448	The incorporation of starch nanocrystals	432:471	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	8	3	theme	antioxidant	1284:1294	arg1	properties					1336:1345	the antibacterial and antioxidant (threefold increase compare to control) properties	1262:1345	the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films	1262:1373	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	4	4	theme	films	763:767	arg1	properties					735:744	the thermal properties	723:744	the thermal properties of the composite films	723:767	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	3	5	theme	FT-IR	495:499	arg1	spectra					501:507	FT-IR spectra	495:507	FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number	495:607	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	9	6	theme	Mucilage-SNC	1376:1387	arg1	films					1399:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	6	theme	Mucilage-SNC	1376:1387	arg1	gain					1433:1436	a good therapeutic gain	1414:1436	a good therapeutic gain for control and directed drug delivery, food packaging, food coating	1414:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	3	7	theme	OH	523:524	arg1	peak					526:529	broad OH peak	517:529	broad OH peak	517:529	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	8	8	theme	composite	1359:1367	arg1	films					1369:1373	mucilage composite films	1350:1373	mucilage composite films	1350:1373	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	9	9	theme	good	1416:1419	arg1	films					1399:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	9	theme	good	1416:1419	arg1	gain					1433:1436	a good therapeutic gain	1414:1436	a good therapeutic gain for control and directed drug delivery, food packaging, food coating	1414:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	4	10	theme	3	669:669	arg1	%					670:670	%	670:670	%	670:670	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	5	11	theme	due	866:868	arg1	nanocrystals					853:864	starch nanocrystals	846:864	starch nanocrystals due to the strong interaction between mucilage and nanocrystals	846:928	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	1	12	theme	current	144:150	arg1	study					152:156	the current study	140:156	the current study	140:156	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	6	13	theme	other	938:942	arg1	hand					944:947	the other hand	934:947	the other hand	934:947	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	3	14	theme	NH	562:563	arg1	vibrations					573:582	NH bending vibrations	562:582	NH bending vibrations	562:582	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	8	15	theme	Starch	1233:1238	arg1	nanocrystals					1240:1251	Starch nanocrystals	1233:1251	Starch nanocrystals	1233:1251	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	1	16	theme	mucilage	164:171	arg1	films					183:187	chia mucilage composite films	159:187	chia mucilage composite films with starch nanocrystals (3% and 6%)	159:224	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	0	17	theme	biological	93:102	arg1	perspective					124:134	An inclusive biological and physicochemical perspective	80:134	An inclusive biological and physicochemical perspective	80:134	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	5	18	theme	strong	877:882	arg1	interaction					884:894	the strong interaction	873:894	the strong interaction between mucilage and nanocrystals	873:928	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	4	19	theme	%	701:701	arg1	289.41 °C					712:720	6% mucilage 289.41 °C	700:720	6% mucilage 289.41 °C	700:720	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	2	20	theme	contact	342:348	arg1	angle					350:354	contact angle	342:354	contact angle	342:354	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	0	21	theme	physicochemical	108:122	arg1	perspective					124:134	An inclusive biological and physicochemical perspective	80:134	An inclusive biological and physicochemical perspective	80:134	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	0	22	theme	films	48:52	arg1	Production					0:9	Production	0:9	Production of novel chia-mucilage nanocomposite films with starch nanocrystals	0:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	9	23	theme	food	1478:1481	arg1	delivery					1468:1475	directed drug delivery	1454:1475	directed drug delivery	1454:1475	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	23	theme	food	1478:1481	arg1	packaging					1483:1491	food packaging	1478:1491	food packaging	1478:1491	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	6	24	theme	composite	989:997	arg1	film					999:1002	mucilage composite film	980:1002	mucilage composite film	980:1002	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	4	25	theme	SNC	672:674	arg1	286.91 °C					676:684	3% SNC 286.91 °C	669:684	3% SNC 286.91 °C	669:684	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	5	26	theme	nanocrystals	853:864	arg1	incorporation					829:841	the incorporation	825:841	the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals	825:928	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	8	27	theme	threefold	1297:1305	arg1	increase					1307:1314	threefold increase	1297:1314	threefold increase compare to control	1297:1333	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	3	28	from	stretching	538:547	arg1	vibrations					573:582	NH bending vibrations	562:582	NH bending vibrations	562:582	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	0	29	theme	novel	14:18	arg1	films					48:52	novel chia-mucilage nanocomposite films	14:52	novel chia-mucilage nanocomposite films with starch nanocrystals	14:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	6	30	theme	overall	954:960	arg1	hydrophobicity					962:975	the overall hydrophobicity	950:975	the overall hydrophobicity of mucilage composite film	950:1002	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	3	31	theme	wave	597:600	arg1	number					602:607	the lower wave number	587:607	the lower wave number	587:607	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	3	32	from	shift	553:557	arg1	vibrations					573:582	NH bending vibrations	562:582	NH bending vibrations	562:582	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	1	33	theme	composite	173:181	arg1	films					183:187	chia mucilage composite films	159:187	chia mucilage composite films with starch nanocrystals (3% and 6%)	159:224	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	0	34	theme	nanocomposite	34:46	arg1	films					48:52	novel chia-mucilage nanocomposite films	14:52	novel chia-mucilage nanocomposite films with starch nanocrystals	14:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	7	35	theme	cell	1215:1218	arg1	line					1220:1223	epithelial cell line	1204:1223	epithelial cell line (Vero)	1204:1230	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	7	35	theme	cell	1215:1218	arg1	Vero					1226:1229	Vero	1226:1229	Vero	1226:1229	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	1	36	with	films	183:187	arg1	nanocrystals					201:212	starch nanocrystals	194:212	starch nanocrystals (3% and 6%)	194:224	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	6	37	theme	hydrophilic	1025:1035	arg1	nature					1037:1042	the hydrophilic nature	1021:1042	the hydrophilic nature of cornstarch nanocrystals	1021:1069	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	8	38	theme	mucilage	1350:1357	arg1	films					1369:1373	mucilage composite films	1350:1373	mucilage composite films	1350:1373	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	7	39	theme	cell	1147:1150	arg1	lines					1160:1164	cancer cell (HepG2) lines	1140:1164	cancer cell (HepG2) lines	1140:1164	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	3	40	theme	starch	453:458	arg1	nanocrystals					460:471	starch nanocrystals	453:471	starch nanocrystals	453:471	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	2	41	dep	physicochemically	265:281	arg1	FT-IR					284:288	FT-IR	284:288	FT-IR	284:288	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	41	dep	physicochemically	265:281	arg1	DSC					301:303	DSC	301:303	DSC	301:303	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	41	dep	physicochemically	265:281	arg1	TGA					296:298	TGA	296:298	TGA	296:298	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	41	dep	physicochemically	265:281	arg1	AFM					291:293	AFM	291:293	AFM	291:293	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	4	42	dep	film	659:662	arg1	control					640:646	control 287.23 °C	640:656	control 287.23 °C	640:656	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	5	43	theme	Young	774:778	arg1	Modulus					780:786	The Young Modulus	770:786	The Young Modulus of the film	770:798	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	4	44	theme	composite	753:761	arg1	films					763:767	the composite films	749:767	the composite films	749:767	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	8	45	dep	antioxidant	1284:1294	arg1	increase					1307:1314	threefold increase	1297:1314	threefold increase compare to control	1297:1333	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	4	46	theme	Starch	610:615	arg1	nanocrystals					617:628	Starch nanocrystals	610:628	Starch nanocrystals	610:628	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	3	47	theme	broad	517:521	arg1	peak					526:529	broad OH peak	517:529	broad OH peak	517:529	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	9	48	theme	composite	1389:1397	arg1	films					1399:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	48	theme	composite	1389:1397	arg1	gain					1433:1436	a good therapeutic gain	1414:1436	a good therapeutic gain for control and directed drug delivery, food packaging, food coating	1414:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	8	49	theme	films	1369:1373	arg1	properties					1336:1345	the antibacterial and antioxidant (threefold increase compare to control) properties	1262:1345	the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films	1262:1373	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	6	50	theme	nanocrystals	1058:1069	arg1	nature					1037:1042	the hydrophilic nature	1021:1042	the hydrophilic nature of cornstarch nanocrystals	1021:1069	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	9	51	theme	directed	1454:1461	arg1	delivery					1468:1475	directed drug delivery	1454:1475	directed drug delivery	1454:1475	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	51	theme	directed	1454:1461	arg1	packaging					1483:1491	food packaging	1478:1491	food packaging	1478:1491	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	51	theme	directed	1454:1461	arg1	coating					1499:1505	food coating	1494:1505	food coating	1494:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	4	52	with	film	690:693	arg1	289.41 °C					712:720	6% mucilage 289.41 °C	700:720	6% mucilage 289.41 °C	700:720	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	1	53	theme	starch	194:199	arg1	nanocrystals					201:212	starch nanocrystals	194:212	starch nanocrystals (3% and 6%)	194:224	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	8	54	dep	increase	1307:1314	arg1	compare					1316:1322	compare	1316:1322	compare to control	1316:1333	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	4	55	with	286.91 °C	676:684	arg1	289.41 °C					712:720	6% mucilage 289.41 °C	700:720	6% mucilage 289.41 °C	700:720	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	3	56	theme	CO	535:536	arg1	stretching					538:547	CO stretching	535:547	CO stretching	535:547	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	3	57	theme	bending	565:571	arg1	vibrations					573:582	NH bending vibrations	562:582	NH bending vibrations	562:582	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	9	58	theme	therapeutic	1421:1431	arg1	films					1399:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films	1376:1403	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	58	theme	therapeutic	1421:1431	arg1	gain					1433:1436	a good therapeutic gain	1414:1436	a good therapeutic gain for control and directed drug delivery, food packaging, food coating	1414:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	59	theme	drug	1463:1466	arg1	delivery					1468:1475	directed drug delivery	1454:1475	directed drug delivery	1454:1475	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	59	theme	drug	1463:1466	arg1	packaging					1483:1491	food packaging	1478:1491	food packaging	1478:1491	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	59	theme	drug	1463:1466	arg1	coating					1499:1505	food coating	1494:1505	food coating	1494:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	3	60	from	peak	526:529	arg1	vibrations					573:582	NH bending vibrations	562:582	NH bending vibrations	562:582	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	7	61	theme	HepG2	1153:1157	arg1	lines					1160:1164	cancer cell (HepG2) lines	1140:1164	cancer cell (HepG2) lines	1140:1164	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	5	62	theme	film	795:798	arg1	Modulus					780:786	The Young Modulus	770:786	The Young Modulus of the film	770:798	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	7	63	theme	cancer	1140:1145	arg1	lines					1160:1164	cancer cell (HepG2) lines	1140:1164	cancer cell (HepG2) lines	1140:1164	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	0	64	theme	inclusive	83:91	arg1	perspective					124:134	An inclusive biological and physicochemical perspective	80:134	An inclusive biological and physicochemical perspective	80:134	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	1	65	theme	chia	159:162	arg1	films					183:187	chia mucilage composite films	159:187	chia mucilage composite films with starch nanocrystals (3% and 6%)	159:224	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	0	66	dep	perspective	124:134	arg1	Production					0:9	Production	0:9	Production of novel chia-mucilage nanocomposite films with starch nanocrystals	0:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	7	67	theme	low	1186:1188	arg1	inhibition					1190:1199	a very low inhibition	1179:1199	a very low inhibition of epithelial cell line (Vero)	1179:1230	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	7	68	theme	significant	1114:1124	arg1	inhibition					1126:1135	significant inhibition	1114:1135	significant inhibition of cancer cell (HepG2) lines	1114:1164	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	6	69	theme	film	999:1002	arg1	hydrophobicity					962:975	the overall hydrophobicity	950:975	the overall hydrophobicity of mucilage composite film	950:1002	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	4	70	theme	6	700:700	arg1	%					701:701	%	701:701	%	701:701	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	5	71	theme	starch	846:851	arg1	nanocrystals					853:864	starch nanocrystals	846:864	starch nanocrystals due to the strong interaction between mucilage and nanocrystals	846:928	The Young Modulus of the film showed an increase after the incorporation of starch nanocrystals due to the strong interaction between mucilage and nanocrystals.
31022490	6	72	theme	mucilage	980:987	arg1	film					999:1002	mucilage composite film	980:1002	mucilage composite film	980:1002	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	4	73	theme	%	670:670	arg1	286.91 °C					676:684	3% SNC 286.91 °C	669:684	3% SNC 286.91 °C	669:684	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	2	74	theme	Tensile	321:327	arg1	strength					329:336	Tensile strength	321:336	Tensile strength	321:336	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	0	75	theme	chia-mucilage	20:32	arg1	films					48:52	novel chia-mucilage nanocomposite films	14:52	novel chia-mucilage nanocomposite films with starch nanocrystals	14:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	1	76	dep	nanocrystals	201:212	arg1	%					223:223	6%	222:223	6%	222:223	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	1	76	dep	nanocrystals	201:212	arg1	%					216:216	3%	215:216	3%	215:216	In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced.
31022490	9	77	theme	food	1494:1497	arg1	delivery					1468:1475	directed drug delivery	1454:1475	directed drug delivery	1454:1475	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	9	77	theme	food	1494:1497	arg1	coating					1499:1505	food coating	1494:1505	food coating	1494:1505	Mucilage-SNC composite films could be a good therapeutic gain for control and directed drug delivery, food packaging, food coating.
31022490	7	78	theme	line	1220:1223	arg1	inhibition					1190:1199	a very low inhibition	1179:1199	a very low inhibition of epithelial cell line (Vero)	1179:1230	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	8	79	theme	antibacterial	1266:1278	arg1	properties					1336:1345	the antibacterial and antioxidant (threefold increase compare to control) properties	1262:1345	the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films	1262:1373	Starch nanocrystals enhanced the antibacterial and antioxidant (threefold increase compare to control) properties of mucilage composite films.
31022490	3	80	theme	lower	591:595	arg1	number					602:607	the lower wave number	587:607	the lower wave number	587:607	The incorporation of starch nanocrystals was confirmed through FT-IR spectra showing broad OH peak and CO stretching and shift in NH bending vibrations to the lower wave number.
31022490	7	81	theme	MTT	1072:1074	arg1	assay					1076:1080	MTT assay	1072:1080	MTT assay for cell proliferation	1072:1103	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	7	82	theme	epithelial	1204:1213	arg1	line					1220:1223	epithelial cell line	1204:1223	epithelial cell line (Vero)	1204:1230	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	7	82	theme	epithelial	1204:1213	arg1	Vero					1226:1229	Vero	1226:1229	Vero	1226:1229	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31022490	4	83	theme	thermal	727:733	arg1	properties					735:744	the thermal properties	723:744	the thermal properties of the composite films	723:767	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	0	84	theme	starch	59:64	arg1	nanocrystals					66:77	starch nanocrystals	59:77	starch nanocrystals	59:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	6	85	theme	cornstarch	1047:1056	arg1	nanocrystals					1058:1069	cornstarch nanocrystals	1047:1069	cornstarch nanocrystals	1047:1069	On the other hand, the overall hydrophobicity of mucilage composite film decreased due to the hydrophilic nature of cornstarch nanocrystals.
31022490	2	86	dep	and	357:359	arg1	cytotoxicity					406:417	cytotoxicity	406:417	cytotoxicity	406:417	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	86	dep	and	357:359	arg1	antioxidant					390:400	antioxidant	390:400	antioxidant	390:400	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	86	dep	and	357:359	arg1	strength					329:336	Tensile strength	321:336	Tensile strength	321:336	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	86	dep	and	357:359	arg1	angle					350:354	contact angle	342:354	contact angle	342:354	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	2	86	dep	and	357:359	arg1	antimicrobial					375:387	antimicrobial	375:387	antimicrobial	375:387	The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties.
31022490	4	87	theme	mucilage	703:710	arg1	289.41 °C					712:720	6% mucilage 289.41 °C	700:720	6% mucilage 289.41 °C	700:720	Starch nanocrystals enhanced (control 287.23 °C, film with 3% SNC 286.91 °C and film with 6% mucilage 289.41 °C) the thermal properties of the composite films.
31022490	0	88	with	films	48:52	arg1	nanocrystals					66:77	starch nanocrystals	59:77	starch nanocrystals	59:77	Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.
31022490	7	89	theme	cell	1086:1089	arg1	proliferation					1091:1103	cell proliferation	1086:1103	cell proliferation	1086:1103	MTT assay for cell proliferation revealed significant inhibition of cancer cell (HepG2) lines and exhibits a very low inhibition of epithelial cell line (Vero).
31593366	1	0	dep	calves	295:300	arg1	Black					312:316	Black	312:316	Black	312:316	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	5	1	located	observed	1152:1159	arg2	decrease					1112:1119	the decrease	1108:1119	the decrease in Fibrobacter and Archaea	1108:1146	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	5	1	located	observed	1152:1159	arg1	calves					1168:1173	CEL calves	1164:1173	CEL calves	1164:1173	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	5	1	located	observed	1152:1159	arg1	4 weeks					1184:1190	first 4 weeks	1178:1190	first 4 weeks in Experiment 1	1178:1206	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	4	2	theme	nutrients	905:913	arg1	replacement					873:883	10% replacement	869:883	10% replacement of total digestible nutrients with concentrate	869:930	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	5	3	theme	calves	1076:1081	arg1	composition					1057:1067	the rumen microbial community composition	1027:1067	the rumen microbial community composition of KRA calves in Experiment 2	1027:1097	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	5	4	theme	methanogenic	993:1004	arg1	Archaea					1006:1012	methanogenic Archaea	993:1012	methanogenic Archaea	993:1012	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	1	5	theme	pre-weaning	332:342	arg1	4 weeks					324:330	4 weeks	324:330	4 weeks pre-weaning to 12 to 16 weeks post-weaning	324:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	3	6	theme	daily	604:608	arg1	gain					617:620	Average daily weight gain	596:620	Average daily weight gain	596:620	Average daily weight gain tended to be greater in CEL than in CON, especially after 1 month of weaning.
31593366	1	7	theme	feeding	147:153	arg1	experiments					155:165	two feeding experiments	143:165	two feeding experiments	143:165	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	6	8	theme	modified	1342:1349	arg1	characteristics					1370:1384	modified rumen fermentation characteristics	1342:1384	modified rumen fermentation characteristics	1342:1384	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	9	theme	beta-glucan	1226:1236	arg1	supplementation					1248:1262	beta-glucan prebiotic supplementation	1226:1262	beta-glucan prebiotic supplementation to grazing calves at pre-weaning	1226:1295	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	1	10	theme	growth	260:265	arg1	performance					267:277	growth performance	260:277	growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	260:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	5	11	theme	bacteria	962:969	arg1	proportion					937:946	The proportion	933:946	The proportion of fibrolytic bacteria	933:969	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	6	12	theme	better	1400:1405	arg1	environment					1413:1423	a better rumen environment	1398:1423	a better rumen environment via different means	1398:1443	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	13	from	pre-weaning	1285:1295	arg1	supplementation					1248:1262	beta-glucan prebiotic supplementation	1226:1262	beta-glucan prebiotic supplementation to grazing calves at pre-weaning	1226:1295	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	4	14	theme	10	869:870	arg1	%					871:871	%	871:871	%	871:871	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	0	15	theme	post-weaning	98:109	arg1	period					111:116	post-weaning period	98:116	post-weaning period on pasture	98:127	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	4	16	with	replacement	873:883	arg1	concentrate					920:930	concentrate	920:930	concentrate	920:930	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	2	17	theme	control	474:480	arg1	group					482:486	a control group	472:486	a control group (CON)	472:492	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	17	theme	control	474:480	arg1	CON					489:491	CON	489:491	CON	489:491	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	18	theme	10 g/day	563:570	arg1	rate					555:558	a rate	553:558	a rate of 10 g/day mixed with concentrate	553:593	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	6	19	theme	microbial	1316:1324	arg1	composition					1326:1336	rumen microbial composition	1310:1336	rumen microbial composition	1310:1336	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	20	theme	different	1429:1437	arg1	means					1439:1443	different means	1429:1443	different means	1429:1443	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	21	theme	prebiotic	1238:1246	arg1	supplementation					1248:1262	beta-glucan prebiotic supplementation	1226:1262	beta-glucan prebiotic supplementation to grazing calves at pre-weaning	1226:1295	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	22	theme	rumen	1351:1355	arg1	characteristics					1370:1384	modified rumen fermentation characteristics	1342:1384	modified rumen fermentation characteristics	1342:1384	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	6	23	theme	grazing	1267:1273	arg1	calves					1275:1280	grazing calves	1267:1280	grazing calves	1267:1280	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	5	24	from	decrease	1112:1119	arg1	Archaea					1140:1146	Archaea	1140:1146	Archaea	1140:1146	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	5	24	from	decrease	1112:1119	arg1	Fibrobacter					1124:1134	Fibrobacter	1124:1134	Fibrobacter	1124:1134	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	4	25	theme	replacement	873:883	arg1	rate					861:864	a rate	859:864	a rate of 10% replacement of total digestible nutrients with concentrate	859:930	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	5	26	theme	community	1047:1055	arg1	composition					1057:1067	the rumen microbial community composition	1027:1067	the rumen microbial community composition of KRA calves in Experiment 2	1027:1097	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	4	27	theme	2.0-month-old	747:759	arg1	calves					739:744	10 calves	736:744	10 calves (2.0-month-old females)	736:768	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	4	27	theme	2.0-month-old	747:759	arg1	females					761:767	2.0-month-old females	747:767	2.0-month-old females	747:767	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	4	28	theme	%	871:871	arg1	replacement					873:883	10% replacement	869:883	10% replacement of total digestible nutrients with concentrate	869:930	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	3	29	theme	Average	596:602	arg1	gain					617:620	Average daily weight gain	596:620	Average daily weight gain	596:620	Average daily weight gain tended to be greater in CEL than in CON, especially after 1 month of weaning.
31593366	0	30	theme	prebiotics	25:34	arg1	Provision					0:8	Provision	0:8	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.	0:128	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	1	31	theme	grazing	282:288	arg1	calves					295:300	grazing beef calves	282:300	grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	282:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	32	with	supplementation	194:208	arg1	cellooligosaccharide					222:241	cellooligosaccharide	222:241	cellooligosaccharide	222:241	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	32	with	supplementation	194:208	arg1	pulp					252:255	kraft pulp	246:255	kraft pulp	246:255	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	6	33	theme	rumen	1407:1411	arg1	environment					1413:1423	a better rumen environment	1398:1423	a better rumen environment via different means	1398:1443	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	0	34	theme	beta-glucan	13:23	arg1	prebiotics					25:34	beta-glucan prebiotics	13:34	beta-glucan prebiotics (cellooligosaccharides and kraft pulp)	13:73	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	1	35	theme	beef	290:293	arg1	calves					295:300	grazing beef calves	282:300	grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	282:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	36	from	performance	267:277	arg1	calves					295:300	grazing beef calves	282:300	grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	282:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	36	from	performance	267:277	arg1	4 weeks					324:330	4 weeks	324:330	4 weeks pre-weaning to 12 to 16 weeks post-weaning	324:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	5	37	theme	first	1178:1182	arg1	4 weeks					1184:1190	first 4 weeks	1178:1190	first 4 weeks in Experiment 1	1178:1206	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	5	38	theme	KRA	1072:1074	arg1	calves					1076:1081	KRA calves	1072:1081	KRA calves	1072:1081	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	2	39	theme	20-week	393:399	arg1	Experiment					379:388	Experiment 1	379:390	Experiment 1 (20-week duration)	379:409	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	39	theme	20-week	393:399	arg1	duration					401:408	20-week duration	393:408	20-week duration	393:408	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	1	40	theme	supplementation	194:208	arg1	effects					183:189	the effects	179:189	the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	179:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	41	dep	16 weeks	353:360	arg1	to					350:351	to	350:351	to	350:351	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	4	42	theme	experimental	816:827	arg1	KRA					836:838	KRA	836:838	KRA	836:838	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	4	42	theme	experimental	816:827	arg1	group					829:833	an experimental group	813:833	an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate	813:930	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	4	43	theme	digestible	894:903	arg1	nutrients					905:913	total digestible nutrients	888:913	total digestible nutrients	888:913	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	5	44	theme	microbial	1037:1045	arg1	composition					1057:1067	the rumen microbial community composition	1027:1067	the rumen microbial community composition of KRA calves in Experiment 2	1027:1097	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	0	45	theme	kraft	63:67	arg1	pulp					69:72	kraft pulp	63:72	kraft pulp	63:72	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	5	46	theme	fibrolytic	951:960	arg1	bacteria					962:969	fibrolytic bacteria	951:969	fibrolytic bacteria	951:969	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	1	47	from	4 weeks	324:330	arg1	calves					295:300	grazing beef calves	282:300	grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	282:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	1	47	from	4 weeks	324:330	arg1	performance					267:277	growth performance	260:277	growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	260:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	5	48	theme	rumen	1031:1035	arg1	composition					1057:1067	the rumen microbial community composition	1027:1067	the rumen microbial community composition of KRA calves in Experiment 2	1027:1097	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	2	49	theme	experimental	500:511	arg1	CEL					520:522	CEL	520:522	CEL	520:522	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	49	theme	experimental	500:511	arg1	group					513:517	an experimental group	497:517	an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate	497:593	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	50	theme	2.9-month-old	425:437	arg1	calves					417:422	nine calves	412:422	nine calves (2.9-month-old females)	412:446	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	2	50	theme	2.9-month-old	425:437	arg1	females					439:445	2.9-month-old females	425:445	2.9-month-old females	425:445	In Experiment 1 (20-week duration), nine calves (2.9-month-old females) were assigned to either a control group (CON) or an experimental group (CEL) fed cellooligosaccharide at a rate of 10 g/day mixed with concentrate.
31593366	1	51	from	effects	183:189	arg1	performance					267:277	growth performance	260:277	growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning	260:373	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31593366	3	52	theme	weaning	691:697	arg1	1 month					680:686	1 month	680:686	1 month of weaning	680:697	Average daily weight gain tended to be greater in CEL than in CON, especially after 1 month of weaning.
31593366	4	53	theme	total	888:892	arg1	nutrients					905:913	total digestible nutrients	888:913	total digestible nutrients	888:913	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	0	54	dep	prebiotics	25:34	arg1	cellooligosaccharides					37:57	cellooligosaccharides	37:57	cellooligosaccharides	37:57	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	0	54	dep	prebiotics	25:34	arg1	pulp					69:72	kraft pulp	63:72	kraft pulp	63:72	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	5	55	from	4 weeks	1184:1190	arg1	Experiment					1195:1204	Experiment 1	1195:1206	Experiment 1	1195:1206	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	6	56	theme	rumen	1310:1314	arg1	composition					1326:1336	rumen microbial composition	1310:1336	rumen microbial composition	1310:1336	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	4	57	theme	control	796:802	arg1	group					804:808	a control group	794:808	a control group	794:808	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	0	58	from	pre-	90:93	arg1	calves					78:83	calves	78:83	calves from pre- to post-weaning period on pasture	78:127	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	5	59	from	composition	1057:1067	arg1	Experiment					1086:1095	Experiment 2	1086:1097	Experiment 2	1086:1097	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	3	60	theme	weight	610:615	arg1	gain					617:620	Average daily weight gain	596:620	Average daily weight gain	596:620	Average daily weight gain tended to be greater in CEL than in CON, especially after 1 month of weaning.
31593366	4	61	theme	16-week	717:723	arg1	Experiment					703:712	Experiment 2	703:714	Experiment 2 (16-week duration)	703:733	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	4	61	theme	16-week	717:723	arg1	duration					725:732	16-week duration	717:732	16-week duration	717:732	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	5	62	theme	CEL	1164:1166	arg1	calves					1168:1173	CEL calves	1164:1173	CEL calves	1164:1173	The proportion of fibrolytic bacteria increased and that of methanogenic Archaea decreased in the rumen microbial community composition of KRA calves in Experiment 2, whereas the decrease in Fibrobacter and Archaea was observed in CEL calves at first 4 weeks in Experiment 1.
31593366	4	63	theme	kraft	845:849	arg1	pulp					851:854	kraft pulp	845:854	kraft pulp	845:854	In Experiment 2 (16-week duration), 10 calves (2.0-month-old females) were assigned to either a control group or an experimental group (KRA) fed kraft pulp at a rate of 10% replacement of total digestible nutrients with concentrate.
31593366	0	64	from	period	111:116	arg1	pasture					121:127	pasture	121:127	pasture	121:127	Provision of beta-glucan prebiotics (cellooligosaccharides and kraft pulp) to calves from pre- to post-weaning period on pasture.
31593366	6	65	theme	fermentation	1357:1368	arg1	characteristics					1370:1384	modified rumen fermentation characteristics	1342:1384	modified rumen fermentation characteristics	1342:1384	We conclude that beta-glucan prebiotic supplementation to grazing calves at pre-weaning would affect rumen microbial composition and modified rumen fermentation characteristics, leading to a better rumen environment via different means.
31593366	1	66	theme	kraft	246:250	arg1	pulp					252:255	kraft pulp	246:255	kraft pulp	246:255	We conducted two feeding experiments to evaluate the effects of supplementation with either cellooligosaccharide or kraft pulp on growth performance in grazing beef calves (Japanese Black) from 4 weeks pre-weaning to 12 to 16 weeks post-weaning.
31558340	0	0	theme	nanofibrillation	90:105	arg1	efficiency					107:116	the cellulose nanofibrillation efficiency	76:116	the cellulose nanofibrillation efficiency	76:116	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	2	1	theme	cellulose	533:541	arg1	nanofibrillation					543:558	cellulose nanofibrillation	533:558	cellulose nanofibrillation	533:558	Compared to pure mechanical fibrillation, both mixed enzymatic pretreatment and post-treatment could efficiently facilitate cellulose nanofibrillation.
31558340	4	2	theme	new	754:756	arg1	insights					758:765	new insights	754:765	new insights into a more efficient biological method for the production and application of cellulose nanomaterials	754:867	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	0	3	theme	cellulose	80:88	arg1	efficiency					107:116	the cellulose nanofibrillation efficiency	76:116	the cellulose nanofibrillation efficiency	76:116	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	1	4	theme	chemical	280:287	arg1	composition					289:299	the chemical composition	276:299	the chemical composition	276:299	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	4	5	dep	production	815:824	arg1	the					811:813	the	811:813	the	811:813	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	1	6	theme	obtained	379:386	arg1	LCNF					388:391	the obtained LCNF	375:391	the obtained LCNF	375:391	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	7	theme	mechanical	182:191	arg1	fibrillation					193:204	mechanical fibrillation	182:204	mechanical fibrillation with mixed enzymatic pretreatment or post-treatment	182:256	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	4	8	theme	biological	789:798	arg1	method					800:805	a more efficient biological method	772:805	a more efficient biological method for the production and application of cellulose nanomaterials	772:867	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	2	9	theme	enzymatic	462:470	arg1	pretreatment					472:483	mixed enzymatic pretreatment	456:483	mixed enzymatic pretreatment	456:483	Compared to pure mechanical fibrillation, both mixed enzymatic pretreatment and post-treatment could efficiently facilitate cellulose nanofibrillation.
31558340	1	10	theme	water	302:306	arg1	value					318:322	water retention value	302:322	water retention value (WRV)	302:328	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	10	theme	water	302:306	arg1	WRV					325:327	WRV	325:327	WRV	325:327	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	4	11	theme	efficient	779:787	arg1	method					800:805	a more efficient biological method	772:805	a more efficient biological method for the production and application of cellulose nanomaterials	772:867	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	2	12	theme	mixed	456:460	arg1	pretreatment					472:483	mixed enzymatic pretreatment	456:483	mixed enzymatic pretreatment	456:483	Compared to pure mechanical fibrillation, both mixed enzymatic pretreatment and post-treatment could efficiently facilitate cellulose nanofibrillation.
31558340	1	13	theme	retention	308:316	arg1	value					318:322	water retention value	302:322	water retention value (WRV)	302:328	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	13	theme	retention	308:316	arg1	WRV					325:327	WRV	325:327	WRV	325:327	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	14	theme	mixed	211:215	arg1	pretreatment					227:238	mixed enzymatic pretreatment	211:238	mixed enzymatic pretreatment	211:238	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	0	15	theme	mixed	14:18	arg1	pretreatment					30:41	mixed enzymatic pretreatment	14:41	mixed enzymatic pretreatment	14:41	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	3	16	theme	smaller	688:694	arg1	height					711:716	smaller average-number height	688:716	smaller average-number height of 15 nm	688:725	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	3	17	theme	LCNF	622:625	arg1	production					627:636	LCNF production	622:636	LCNF production	622:636	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	1	18	theme	enzymatic	217:225	arg1	pretreatment					227:238	mixed enzymatic pretreatment	211:238	mixed enzymatic pretreatment	211:238	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	3	19	theme	average-number	696:709	arg1	height					711:716	smaller average-number height	688:716	smaller average-number height of 15 nm	688:725	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	2	20	theme	mechanical	426:435	arg1	fibrillation					437:448	pure mechanical fibrillation	421:448	pure mechanical fibrillation	421:448	Compared to pure mechanical fibrillation, both mixed enzymatic pretreatment and post-treatment could efficiently facilitate cellulose nanofibrillation.
31558340	0	21	theme	pretreatment	30:41	arg1	Comparison					0:9	Comparison	0:9	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.	0:117	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	3	22	theme	%	682:682	arg1	WRV					672:674	a relatively higher WRV	652:674	a relatively higher WRV of 909%	652:682	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	3	22	theme	%	682:682	arg1	height					711:716	smaller average-number height	688:716	smaller average-number height of 15 nm	688:725	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	0	23	theme	enzymatic	20:28	arg1	pretreatment					30:41	mixed enzymatic pretreatment	14:41	mixed enzymatic pretreatment	14:41	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	2	24	theme	pure	421:424	arg1	fibrillation					437:448	pure mechanical fibrillation	421:448	pure mechanical fibrillation	421:448	Compared to pure mechanical fibrillation, both mixed enzymatic pretreatment and post-treatment could efficiently facilitate cellulose nanofibrillation.
31558340	1	25	with	fibrillation	193:204	arg1	post-treatment					243:256	post-treatment	243:256	post-treatment	243:256	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	25	with	fibrillation	193:204	arg1	pretreatment					227:238	mixed enzymatic pretreatment	211:238	mixed enzymatic pretreatment	211:238	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	0	26	theme	post-treatment	47:60	arg1	Comparison					0:9	Comparison	0:9	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.	0:117	Comparison of mixed enzymatic pretreatment and post-treatment for enhancing the cellulose nanofibrillation efficiency.
31558340	3	27	theme	15 nm	721:725	arg1	WRV					672:674	a relatively higher WRV	652:674	a relatively higher WRV of 909%	652:682	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	3	27	theme	15 nm	721:725	arg1	height					711:716	smaller average-number height	688:716	smaller average-number height of 15 nm	688:725	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	1	28	theme	average-number	331:344	arg1	height					346:351	average-number height	331:351	average-number height	331:351	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	4	29	theme	cellulose	845:853	arg1	nanomaterials					855:867	cellulose nanomaterials	845:867	cellulose nanomaterials	845:867	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	1	30	theme	lignocellulosic	133:147	arg1	LCNF					162:165	LCNF	162:165	LCNF	162:165	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	1	30	theme	lignocellulosic	133:147	arg1	nanofibrils					149:159	lignocellulosic nanofibrils	133:159	lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment	133:256	In this work, lignocellulosic nanofibrils (LCNF) produced from mechanical fibrillation with mixed enzymatic pretreatment or post-treatment were compared and the chemical composition, water retention value (WRV), average-number height and crystallinity for the obtained LCNF were evaluated.
31558340	3	31	theme	higher	665:670	arg1	WRV					672:674	a relatively higher WRV	652:674	a relatively higher WRV of 909%	652:682	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	3	32	theme	mixed	571:575	arg1	pretreatment					587:598	mixed enzymatic pretreatment	571:598	mixed enzymatic pretreatment	571:598	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31558340	4	33	theme	nanomaterials	855:867	arg1	production					815:824	production	815:824	production	815:824	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	4	33	theme	nanomaterials	855:867	arg1	application					830:840	application	830:840	application	830:840	These discoveries provide new insights into a more efficient biological method for the production and application of cellulose nanomaterials.
31558340	3	34	theme	enzymatic	577:585	arg1	pretreatment					587:598	mixed enzymatic pretreatment	571:598	mixed enzymatic pretreatment	571:598	Moreover, mixed enzymatic pretreatment was more suitable for LCNF production, resulting in a relatively higher WRV of 909% and smaller average-number height of 15 nm.
31472860	3	0	theme	interacting	796:806	arg1	network					812:818	a well-dispersed and strongly interacting CNF network	766:818	a well-dispersed and strongly interacting CNF network	766:818	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	3	1	theme	rheological	651:661	arg1	analyses					663:670	rheological analyses	651:670	rheological analyses	651:670	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	0	2	theme	carrier	114:120	arg1	system					122:127	a sustainable polyethylene glycol-based carrier system	74:127	a sustainable polyethylene glycol-based carrier system	74:127	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	5	3	theme	thermal	1211:1217	arg1	stability					1219:1227	the PLA thermal stability	1203:1227	the PLA thermal stability	1203:1227	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	2	4	theme	drying	532:537	arg1	way					539:541	a gentle drying way	523:541	a gentle drying way	523:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	4	5	theme	CNF	964:966	arg1	network					968:974	the web-like CNF network	951:974	the web-like CNF network	951:974	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	5	6	theme	Thermogravimetric	1045:1061	arg1	analysis					1063:1070	Thermogravimetric analysis	1045:1070	Thermogravimetric analysis	1045:1070	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	6	7	theme	CNF	1349:1351	arg1	masterbatches					1353:1365	melt-processable CNF masterbatches	1332:1365	melt-processable CNF masterbatches	1332:1365	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	2	8	theme	gentle	525:530	arg1	way					539:541	a gentle drying way	523:541	a gentle drying way	523:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	4	9	theme	web-like	955:962	arg1	network					968:974	the web-like CNF network	951:974	the web-like CNF network	951:974	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	5	10	from	case	1094:1097	arg1	interface					1139:1147	the PLA-PEG interface	1127:1147	the PLA-PEG interface	1127:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	5	11	theme	PEG	1187:1189	arg1	addition					1191:1198	PEG addition	1187:1198	PEG addition	1187:1198	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	6	12	theme	scalable	1430:1437	arg1	techniques					1455:1464	conventional industrially scalable melt-processing techniques	1404:1464	conventional industrially scalable melt-processing techniques	1404:1464	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	5	13	theme	addition	1191:1198	arg1	impact					1177:1182	the negative impact	1164:1182	the negative impact of PEG addition on the PLA thermal stability	1164:1227	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	5	14	theme	PLA	1207:1209	arg1	stability					1219:1227	the PLA thermal stability	1203:1227	the PLA thermal stability	1203:1227	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	3	15	theme	well-dispersed	768:781	arg1	network					812:818	a well-dispersed and strongly interacting CNF network	766:818	a well-dispersed and strongly interacting CNF network	766:818	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	4	16	theme	network	968:974	arg1	capacity					939:946	the load-bearing capacity	922:946	the load-bearing capacity of the web-like CNF network	922:974	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	4	16	theme	network	968:974	arg1	apparent					990:997	apparent	990:997	apparent	990:997	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	3	17	theme	network	812:818	arg1	formation					753:761	the formation	749:761	the formation of a well-dispersed and strongly interacting CNF network	749:818	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	4	18	theme	load-bearing	926:937	arg1	capacity					939:946	the load-bearing capacity	922:946	the load-bearing capacity of the web-like CNF network	922:974	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	4	18	theme	load-bearing	926:937	arg1	apparent					990:997	apparent	990:997	apparent	990:997	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	5	19	theme	PLA-PEG	1131:1137	arg1	interface					1139:1147	the PLA-PEG interface	1127:1147	the PLA-PEG interface	1127:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	0	20	theme	nanofibril/polylactide	29:50	arg1	Melt-processing					0:14	Melt-processing	0:14	Melt-processing of cellulose nanofibril/polylactide	0:50	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	3	21	theme	Morphological	611:623	arg1	observations					625:636	Morphological observations	611:636	Morphological observations coupled with rheological analyses	611:670	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	5	22	from	interface	1139:1147	arg1	case					1094:1097	the case	1090:1097	the case of selective positioning at the PLA-PEG interface	1090:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	5	22	from	interface	1139:1147	arg1	positioning					1112:1122	selective positioning	1102:1122	selective positioning at the PLA-PEG interface	1102:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	5	23	theme	selective	1102:1110	arg1	positioning					1112:1122	selective positioning	1102:1122	selective positioning at the PLA-PEG interface	1102:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	2	24	theme	aqueous	437:443	arg1	suspension					455:464	an aqueous CNF-based suspension	434:464	an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way	434:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	2	25	theme	CNF-based	445:453	arg1	suspension					455:464	an aqueous CNF-based suspension	434:464	an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way	434:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	1	26	theme	cellulose	318:326	arg1	CNF					341:343	CNF	341:343	CNF	341:343	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	1	26	theme	cellulose	318:326	arg1	nanofibrils					328:338	cellulose nanofibrils	318:338	cellulose nanofibrils (CNF)	318:344	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	2	27	theme	high	385:388	arg1	propensity					390:399	their high propensity to self-aggregate on processing	379:431	their high propensity to self-aggregate on processing	379:431	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	1	28	theme	appealing	146:154	arg1	need					156:159	the appealing need	142:159	the appealing need for an industrially viable approach	142:195	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	1	29	theme	rapid	235:239	arg1	processing					255:264	the rapid and easy melt processing	231:264	the rapid and easy melt processing of Polylactide (PLA)	231:285	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	2	30	theme	glycol	498:503	arg1	presence					473:480	the presence	469:480	the presence of polyethylene glycol (PEG) followed by a gentle drying way	469:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	3	31	theme	CNF	808:810	arg1	network					812:818	a well-dispersed and strongly interacting CNF network	766:818	a well-dispersed and strongly interacting CNF network	766:818	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	4	32	theme	PEG	1020:1022	arg1	effect					1037:1042	the PEG plasticizing effect	1016:1042	the PEG plasticizing effect	1016:1042	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	4	33	theme	thermo-mechanical	875:891	arg1	characterization					893:908	thermo-mechanical characterization	875:908	thermo-mechanical characterization	875:908	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	6	34	theme	sustainable	1279:1289	arg1	approach					1312:1319	our sustainable organic solvent-free approach	1275:1319	our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches	1275:1365	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	2	35	theme	polyethylene	485:496	arg1	PEG					506:508	PEG	506:508	PEG	506:508	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	2	35	theme	polyethylene	485:496	arg1	glycol					498:503	polyethylene glycol	485:503	polyethylene glycol (PEG)	485:509	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	5	36	theme	negative	1168:1175	arg1	impact					1177:1182	the negative impact	1164:1182	the negative impact of PEG addition on the PLA thermal stability	1164:1227	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	5	37	theme	positioning	1112:1122	arg1	case					1094:1097	the case	1090:1097	the case of selective positioning at the PLA-PEG interface	1090:1147	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	1	38	theme	easy	245:248	arg1	processing					255:264	the rapid and easy melt processing	231:264	the rapid and easy melt processing of Polylactide (PLA)	231:285	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	0	39	theme	sustainable	76:86	arg1	system					122:127	a sustainable polyethylene glycol-based carrier system	74:127	a sustainable polyethylene glycol-based carrier system	74:127	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	4	40	theme	plasticizing	1024:1035	arg1	effect					1037:1042	the PEG plasticizing effect	1016:1042	the PEG plasticizing effect	1016:1042	At temperatures above Tg, thermo-mechanical characterization showed that the load-bearing capacity of the web-like CNF network was even more apparent and counteracted the PEG plasticizing effect.
31472860	6	41	theme	melt-processing	1439:1453	arg1	techniques					1455:1464	conventional industrially scalable melt-processing techniques	1404:1464	conventional industrially scalable melt-processing techniques	1404:1464	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	1	42	dep	works	203:207	arg1	aims					209:212	aims	209:212	works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF)	203:344	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	2	43	theme	CNF-based	586:594	arg1	masterbatches					596:608	melt-processable CNF-based masterbatches	569:608	melt-processable CNF-based masterbatches	569:608	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	3	44	theme	masterbatch	716:726	arg1	approach					728:735	the PEG-based masterbatch approach	702:735	the PEG-based masterbatch approach	702:735	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	3	45	theme	approach	728:735	arg1	strategy					690:697	the strategy	686:697	the strategy of the PEG-based masterbatch approach	686:735	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	3	46	theme	polymeric	831:839	arg1	matrix					841:846	the polymeric matrix	827:846	the polymeric matrix	827:846	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	2	47	theme	melt-processable	569:584	arg1	masterbatches					596:608	melt-processable CNF-based masterbatches	569:608	melt-processable CNF-based masterbatches	569:608	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	1	48	dep	bio-composites	287:300	arg1	reinforced					302:311	reinforced	302:311	bio-composites reinforced with cellulose nanofibrils (CNF)	287:344	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	6	49	theme	approach	1312:1319	arg1	successfulness					1257:1270	the successfulness	1253:1270	the successfulness	1253:1270	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	6	50	theme	melt-processable	1332:1347	arg1	masterbatches					1353:1365	melt-processable CNF masterbatches	1332:1365	melt-processable CNF masterbatches	1332:1365	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	1	51	theme	melt	250:253	arg1	processing					255:264	the rapid and easy melt processing	231:264	the rapid and easy melt processing of Polylactide (PLA)	231:285	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	6	52	theme	conventional	1404:1415	arg1	techniques					1455:1464	conventional industrially scalable melt-processing techniques	1404:1464	conventional industrially scalable melt-processing techniques	1404:1464	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	6	53	theme	solvent-free	1299:1310	arg1	approach					1312:1319	our sustainable organic solvent-free approach	1275:1319	our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches	1275:1365	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	5	54	from	impact	1177:1182	arg1	stability					1219:1227	the PLA thermal stability	1203:1227	the PLA thermal stability	1203:1227	Thermogravimetric analysis evidenced that in the case of selective positioning at the PLA-PEG interface, CNF mitigated the negative impact of PEG addition on the PLA thermal stability.
31472860	0	55	theme	glycol-based	101:112	arg1	system					122:127	a sustainable polyethylene glycol-based carrier system	74:127	a sustainable polyethylene glycol-based carrier system	74:127	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	3	56	theme	PEG-based	706:714	arg1	approach					728:735	the PEG-based masterbatch approach	702:735	the PEG-based masterbatch approach	702:735	Morphological observations coupled with rheological analyses confirmed how the strategy of the PEG-based masterbatch approach facilitated the formation of a well-dispersed and strongly interacting CNF network within the polymeric matrix.
31472860	6	57	theme	organic	1291:1297	arg1	approach					1312:1319	our sustainable organic solvent-free approach	1275:1319	our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches	1275:1365	These results revealed the successfulness of our sustainable organic solvent-free approach to prepare melt-processable CNF masterbatches, which can be readily converted into conventional industrially scalable melt-processing techniques.
31472860	1	58	theme	viable	181:186	arg1	approach					188:195	an industrially viable approach	165:195	an industrially viable approach	165:195	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
31472860	0	59	theme	polyethylene	88:99	arg1	system					122:127	a sustainable polyethylene glycol-based carrier system	74:127	a sustainable polyethylene glycol-based carrier system	74:127	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	2	60	from	suspension	455:464	arg1	presence					473:480	the presence	469:480	the presence of polyethylene glycol (PEG) followed by a gentle drying way	469:541	For this purpose and against to their high propensity to self-aggregate on processing, an aqueous CNF-based suspension in the presence of polyethylene glycol (PEG) followed by a gentle drying way were performed to provide melt-processable CNF-based masterbatches.
31472860	0	61	theme	cellulose	19:27	arg1	nanofibril/polylactide					29:50	cellulose nanofibril/polylactide	19:50	cellulose nanofibril/polylactide	19:50	Melt-processing of cellulose nanofibril/polylactide bionanocomposites via a sustainable polyethylene glycol-based carrier system.
31472860	1	62	theme	Polylactide	269:279	arg1	processing					255:264	the rapid and easy melt processing	231:264	the rapid and easy melt processing of Polylactide (PLA)	231:285	Considering the appealing need for an industrially viable approach, this works aims at demonstrating the rapid and easy melt processing of Polylactide (PLA) bio-composites reinforced with cellulose nanofibrils (CNF).
30599996	0	0	theme	enzymatic	83:91	arg1	treatment					93:101	enzymatic treatment	83:101	enzymatic treatment	83:101	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	9	1	theme	barrier	954:960	arg1	opacity					974:980	opacity	974:980	opacity	974:980	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	1	theme	barrier	954:960	arg1	properties					962:971	mechanical and water barrier properties	933:971	properties	962:971	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	1	theme	barrier	954:960	arg1	barrier					995:1001	UV light barrier	986:1001	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	2	theme	due	836:838	arg1	structure					826:834	a complex structure	816:834	a complex structure due to strong interactions between CNFs and starch matrix	816:892	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	10	3	theme	starch-based	1094:1105	arg1	films					1121:1125	starch-based nanocomposite films	1094:1125	starch-based nanocomposite films	1094:1125	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	1	4	theme	cellulose	159:167	arg1	CNFs					181:184	CNFs	181:184	CNFs	181:184	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	1	4	theme	cellulose	159:167	arg1	nanofibers					169:178	cellulose nanofibers	159:178	cellulose nanofibers (CNFs)	159:185	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	3	5	theme	Banana	351:356	arg1	films					385:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films were prepared with CNFs using the casting method.
30599996	9	6	from	improvement	918:928	arg1	opacity					974:980	opacity	974:980	opacity	974:980	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	6	from	improvement	918:928	arg1	properties					962:971	mechanical and water barrier properties	933:971	properties	962:971	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	6	from	improvement	918:928	arg1	barrier					995:1001	UV light barrier	986:1001	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	7	theme	strong	843:848	arg1	interactions					850:861	strong interactions	843:861	strong interactions between CNFs and starch matrix	843:892	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	3	8	theme	starch-based	358:369	arg1	films					385:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films were prepared with CNFs using the casting method.
30599996	8	9	theme	Topography	700:709	arg1	analysis					711:718	Topography analysis	700:718	Topography analysis	700:718	Topography analysis showed that the incorporation of CNFs modified the film structure.
30599996	0	10	with	nanocomposite	14:26	arg1	nanofibers					43:52	cellulose nanofibers	33:52	cellulose nanofibers isolated from banana peel by enzymatic treatment	33:101	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	0	11	dep	In	104:105	arg1	vitro					107:111	vitro	107:111	vitro	107:111	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	1	12	theme	nanofibers	169:178	arg1	use					152:154	The potential use	138:154	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films	138:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	0	13	theme	In	104:105	arg1	assessment					126:135	In vitro cytotoxicity assessment	104:135	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.	0:136	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	4	14	theme	films	492:496	arg1	properties					498:507	nanocomposite films properties	478:507	nanocomposite films properties'	478:508	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	5	15	theme	Caco-2	569:574	arg1	line					581:584	Caco-2 cell line	569:584	Caco-2 cell line	569:584	The cytotoxicity of CNFs was assessed on Caco-2 cell line.
30599996	10	16	theme	waste	1174:1178	arg1	material					1180:1187	a waste material	1172:1187	a waste material	1172:1187	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	0	17	theme	starch	7:12	arg1	nanocomposite					14:26	Banana starch nanocomposite	0:26	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.	0:136	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	4	18	theme	nanocomposite	478:490	arg1	properties					498:507	nanocomposite films properties	478:507	nanocomposite films properties'	478:508	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	5	19	theme	cell	576:579	arg1	line					581:584	Caco-2 cell line	569:584	Caco-2 cell line	569:584	The cytotoxicity of CNFs was assessed on Caco-2 cell line.
30599996	2	20	theme	Musa	308:311	arg1	peel					302:305	banana peel	295:305	banana peel (Musa paradisiaca)	295:324	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	2	20	theme	Musa	308:311	arg1	paradisiaca					313:323	Musa paradisiaca	308:323	Musa paradisiaca	308:323	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	3	21	theme	casting	425:431	arg1	method					433:438	the casting method	421:438	the casting method	421:438	Banana starch-based nanocomposite films were prepared with CNFs using the casting method.
30599996	0	22	theme	Banana	0:5	arg1	nanocomposite					14:26	Banana starch nanocomposite	0:26	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.	0:136	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	9	23	theme	starch	880:885	arg1	matrix					887:892	starch matrix	880:892	starch matrix	880:892	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	0	24	attach	isolated	54:61	arg2	nanofibers					43:52	cellulose nanofibers	33:52	cellulose nanofibers isolated from banana peel by enzymatic treatment	33:101	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	0	24	attach	isolated	54:61	arg1	peel					75:78	banana peel	68:78	banana peel	68:78	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	10	25	theme	reinforcing	1069:1079	arg1	material					1081:1088	reinforcing material	1069:1088	reinforcing material	1069:1088	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	3	26	theme	nanocomposite	371:383	arg1	films					385:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films	351:389	Banana starch-based nanocomposite films were prepared with CNFs using the casting method.
30599996	10	27	theme	food	1152:1155	arg1	packaging					1157:1165	a value-added food packaging	1138:1165	a value-added food packaging	1138:1165	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	0	28	theme	cytotoxicity	113:124	arg1	assessment					126:135	In vitro cytotoxicity assessment	104:135	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.	0:136	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	2	29	theme	banana	295:300	arg1	peel					302:305	banana peel	295:305	banana peel (Musa paradisiaca)	295:324	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	2	29	theme	banana	295:300	arg1	paradisiaca					313:323	Musa paradisiaca	308:323	Musa paradisiaca	308:323	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	10	30	theme	value-added	1140:1150	arg1	packaging					1157:1165	a value-added food packaging	1138:1165	a value-added food packaging	1138:1165	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	1	31	theme	reinforcing	192:202	arg1	agent					204:208	a reinforcing agent	190:208	a reinforcing agent in banana starch-based nanocomposite films	190:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	0	32	theme	cellulose	33:41	arg1	nanofibers					43:52	cellulose nanofibers	33:52	cellulose nanofibers isolated from banana peel by enzymatic treatment	33:101	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	2	33	attach	isolated	281:288	arg2	CNFs					271:274	CNFs	271:274	CNFs	271:274	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	2	33	attach	isolated	281:288	arg1	paradisiaca					313:323	Musa paradisiaca	308:323	Musa paradisiaca	308:323	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	2	33	attach	isolated	281:288	arg1	peel					302:305	banana peel	295:305	banana peel (Musa paradisiaca)	295:324	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	4	34	from	effect	446:451	arg1	properties					498:507	nanocomposite films properties	478:507	nanocomposite films properties'	478:508	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	4	34	from	effect	446:451	arg1	viability					461:469	cell viability	456:469	cell viability	456:469	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	5	35	theme	CNFs	548:551	arg1	cytotoxicity					532:543	The cytotoxicity	528:543	The cytotoxicity of CNFs	528:551	The cytotoxicity of CNFs was assessed on Caco-2 cell line.
30599996	9	36	theme	UV	986:987	arg1	light					989:993	UV light	986:993	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	8	37	theme	CNFs	753:756	arg1	incorporation					736:748	the incorporation	732:748	the incorporation of CNFs	732:756	Topography analysis showed that the incorporation of CNFs modified the film structure.
30599996	9	38	theme	remarkable	907:916	arg1	improvement					918:928	a remarkable improvement	905:928	a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film	905:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	4	39	theme	cell	456:459	arg1	viability					461:469	cell viability	456:469	cell viability	456:469	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	1	40	from	agent	204:208	arg1	films					247:251	banana starch-based nanocomposite films	213:251	banana starch-based nanocomposite films	213:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	2	41	theme	enzymatic	329:337	arg1	hydrolysis					339:348	enzymatic hydrolysis	329:348	enzymatic hydrolysis	329:348	CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis.
30599996	8	42	theme	film	771:774	arg1	structure					776:784	the film structure	767:784	the film structure	767:784	Topography analysis showed that the incorporation of CNFs modified the film structure.
30599996	7	43	theme	cell	684:687	arg1	viability					689:697	cell viability	684:697	cell viability	684:697	However, CNFs above 2000 μg/mL significantly decreased cell viability.
30599996	9	44	theme	control	1019:1025	arg1	film					1027:1030	the control film	1015:1030	the control film	1015:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	6	45	from	50-2000 μg/mL	614:626	arg1	cytotoxic					601:609	cytotoxic	601:609	cytotoxic	601:609	CNFs were not cytotoxic at 50-2000 μg/mL.
30599996	9	46	theme	mechanical	933:942	arg1	opacity					974:980	opacity	974:980	opacity	974:980	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	46	theme	mechanical	933:942	arg1	properties					962:971	mechanical and water barrier properties	933:971	properties	962:971	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	46	theme	mechanical	933:942	arg1	barrier					995:1001	UV light barrier	986:1001	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	4	47	theme	CNFs	441:444	arg1	effect					446:451	CNFs effect	441:451	CNFs effect on cell viability and on nanocomposite films properties'	441:508	CNFs effect on cell viability and on nanocomposite films properties' was investigated.
30599996	0	48	dep	nanocomposite	14:26	arg1	assessment					126:135	In vitro cytotoxicity assessment	104:135	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.	0:136	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	1	49	theme	banana	213:218	arg1	films					247:251	banana starch-based nanocomposite films	213:251	banana starch-based nanocomposite films	213:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	10	50	theme	great	1050:1054	arg1	potential					1056:1064	a great potential	1048:1064	a great potential as reinforcing material for starch-based nanocomposite films	1048:1125	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	9	51	theme	complex	818:824	arg1	structure					826:834	a complex structure	816:834	a complex structure due to strong interactions between CNFs and starch matrix	816:892	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	1	52	theme	potential	142:150	arg1	use					152:154	The potential use	138:154	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films	138:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	1	53	theme	starch-based	220:231	arg1	films					247:251	banana starch-based nanocomposite films	213:251	banana starch-based nanocomposite films	213:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30599996	0	54	theme	banana	68:73	arg1	peel					75:78	banana peel	68:78	banana peel	68:78	Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment.
30599996	10	55	theme	nanocomposite	1107:1119	arg1	films					1121:1125	starch-based nanocomposite films	1094:1125	starch-based nanocomposite films	1094:1125	CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.
30599996	9	56	theme	water	948:952	arg1	opacity					974:980	opacity	974:980	opacity	974:980	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	56	theme	water	948:952	arg1	properties					962:971	mechanical and water barrier properties	933:971	properties	962:971	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	56	theme	water	948:952	arg1	barrier					995:1001	UV light barrier	986:1001	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	57	theme	light	989:993	arg1	properties					962:971	mechanical and water barrier properties	933:971	properties	962:971	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	9	57	theme	light	989:993	arg1	barrier					995:1001	UV light barrier	986:1001	UV light barrier compared to the control film	986:1030	The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film.
30599996	1	58	theme	nanocomposite	233:245	arg1	films					247:251	banana starch-based nanocomposite films	213:251	banana starch-based nanocomposite films	213:251	The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated.
30007891	4	0	theme	hydrogen	842:849	arg1	rates					862:866	high hydrogen production rates	837:866	high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium,	837:989	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	1	1	theme	sugarcane	304:312	arg1	bagasse					314:320	sugarcane bagasse	304:320	sugarcane bagasse (SCB)	304:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	1	theme	sugarcane	304:312	arg1	SCB					323:325	SCB	323:325	SCB	323:325	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	2	theme	mutual	162:167	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	5	3	theme	Significant	1059:1069	arg1	changes					1071:1077	Significant changes	1059:1077	Significant changes	1059:1077	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	0	4	theme	allochthonous	78:90	arg1	bacteria					92:99	autochthonous and allochthonous bacteria	60:99	autochthonous and allochthonous bacteria from sugarcane	60:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	2	5	theme	acid	570:573	arg1	1.57 g/L					584:591	1.57 g/L	584:591	1.57 g/L	584:591	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	2	5	theme	acid	570:573	arg1	pathway					575:581	the acetic acid pathway	559:581	the acetic acid pathway (1.57 g/L)	559:592	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	0	6	from	sugarcane	106:114	arg1	bacteria					92:99	autochthonous and allochthonous bacteria	60:99	autochthonous and allochthonous bacteria from sugarcane	60:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	4	7	theme	Illumina	928:935	arg1	sequencing					937:946	Illumina sequencing	928:946	Illumina sequencing (16S RNAr)	928:957	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	4	7	theme	Illumina	928:935	arg1	RNAr					953:956	16S RNAr	949:956	16S RNAr	949:956	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	2	8	theme	23.10 mmoL	459:468	arg1	H2/L					470:473	23.10 mmoL H2/L	459:473	23.10 mmoL H2/L	459:473	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	1	9	from	7.4	246:248	arg1	concentration					190:202	substrate concentration	180:202	substrate concentration (from 0.8 to 9.2 g/L)	180:224	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	9	from	7.4	246:248	arg1	pH					230:231	pH	230:231	pH (from 4.6 to 7.4)	230:249	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	9	from	7.4	246:248	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	5	10	dep	condition	1213:1221	arg1	pH					1240:1241	pH 6.0	1240:1245	pH 6.0	1240:1245	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	10	dep	condition	1213:1221	arg1	SCB/L					1230:1234	5.0 g SCB/L	1224:1234	5.0 g SCB/L	1224:1234	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	3	11	theme	major	631:635	arg1	1.65 g/L					741:748	1.65 g/L	741:748	1.65 g/L	741:748	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	11	theme	major	631:635	arg1	VFA					659:661	VFA	659:661	VFA	659:661	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	11	theme	major	631:635	arg1	1.88 g/L					715:722	1.88 g/L	715:722	1.88 g/L	715:722	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	11	theme	major	631:635	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	11	theme	major	631:635	arg1	acids					616:620	Butyric and succinic acids	595:620	Butyric and succinic acids	595:620	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	2	12	theme	SCB/L	524:528	arg1	conditions					504:513	optimized conditions	494:513	optimized conditions of 7.0 g SCB/L and pH 7.2	494:539	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	2	13	theme	acetic	563:568	arg1	1.57 g/L					584:591	1.57 g/L	584:591	1.57 g/L	584:591	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	2	13	theme	acetic	563:568	arg1	pathway					575:581	the acetic acid pathway	559:581	the acetic acid pathway (1.57 g/L)	559:592	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	5	14	from	changes	1137:1143	arg1	variables					1164:1172	the independent variables	1148:1172	the independent variables	1148:1172	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	4	15	theme	production	851:860	arg1	rates					862:866	high hydrogen production rates	837:866	high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium,	837:989	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	3	16	theme	volatile	637:644	arg1	1.65 g/L					741:748	1.65 g/L	741:748	1.65 g/L	741:748	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	16	theme	volatile	637:644	arg1	VFA					659:661	VFA	659:661	VFA	659:661	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	16	theme	volatile	637:644	arg1	1.88 g/L					715:722	1.88 g/L	715:722	1.88 g/L	715:722	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	16	theme	volatile	637:644	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	16	theme	volatile	637:644	arg1	acids					616:620	Butyric and succinic acids	595:620	Butyric and succinic acids	595:620	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	17	dep	1.65 g/L	741:748	arg1	to					738:739	to	738:739	to	738:739	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	4	18	theme	useful	813:818	arg1	tools					820:824	useful tools	813:824	useful tools	813:824	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	4	18	theme	useful	813:818	arg1	RSM					796:798	RSM	796:798	RSM	796:798	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	1	19	from	bagasse	314:320	arg1	production					288:297	hydrogen and volatile fatty acids production	254:297	hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	254:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	4	20	theme	Clostridium	995:1005	arg1	bacteria					1040:1047	Clostridium and Paenibacillus, autochthonous bacteria	995:1047	bacteria	1040:1047	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	1	21	theme	substrate	180:188	arg1	concentration					190:202	substrate concentration	180:202	substrate concentration (from 0.8 to 9.2 g/L)	180:224	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	4	22	from	SCB	1054:1056	arg1	rates					862:866	high hydrogen production rates	837:866	high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium,	837:989	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	4	22	from	SCB	1054:1056	arg1	bacteria					1040:1047	Clostridium and Paenibacillus, autochthonous bacteria	995:1047	bacteria	1040:1047	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	4	23	theme	high	837:840	arg1	rates					862:866	high hydrogen production rates	837:866	high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium,	837:989	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	1	24	theme	central	412:418	arg1	CCD					438:440	CCD	438:440	CCD	438:440	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	24	theme	central	412:418	arg1	design					430:435	central composite design	412:435	central composite design (CCD)	412:441	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	5	25	from	genera	1185:1190	arg1	condition					1213:1221	the central point condition	1195:1221	the central point condition (5.0 g SCB/L and pH 6.0)	1195:1246	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	26	theme	point	1207:1211	arg1	condition					1213:1221	the central point condition	1195:1221	the central point condition (5.0 g SCB/L and pH 6.0)	1195:1246	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	1	27	theme	concentration	190:202	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	0	28	theme	Optimization	0:11	arg1	productions					43:53	Optimization of hydrogen and organic acids productions	0:53	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane	0:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	1	29	theme	composite	420:428	arg1	CCD					438:440	CCD	438:440	CCD	438:440	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	29	theme	composite	420:428	arg1	design					430:435	central composite design	412:435	central composite design (CCD)	412:441	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	2	30	theme	pH	534:535	arg1	conditions					504:513	optimized conditions	494:513	optimized conditions of 7.0 g SCB/L and pH 7.2	494:539	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	5	31	theme	7.0 g	1381:1385	arg1	SCB/L					1387:1391	7.0 g SCB/L	1381:1391	7.0 g SCB/L	1381:1391	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	0	32	theme	hydrogen	16:23	arg1	acids					37:41	organic acids	29:41	organic acids	29:41	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	0	32	theme	hydrogen	16:23	arg1	Optimization					0:11	Optimization	0:11	Optimization of hydrogen	0:23	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	1	33	theme	hydrogen	254:261	arg1	production					288:297	hydrogen and volatile fatty acids production	254:297	hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	254:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	0	34	theme	batch	127:131	arg1	reactors					133:140	batch reactors	127:140	batch reactors	127:140	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	4	35	theme	fermentative	966:977	arg1	consortium					979:988	the fermentative consortium	962:988	the fermentative consortium	962:988	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	0	36	theme	acids	37:41	arg1	productions					43:53	Optimization of hydrogen and organic acids productions	0:53	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane	0:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	1	37	theme	batch	349:353	arg1	reactors					355:362	batch reactors	349:362	batch reactors	349:362	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	0	38	theme	organic	29:35	arg1	acids					37:41	organic acids	29:41	organic acids	29:41	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	1	39	theme	volatile	267:274	arg1	acids					282:286	volatile fatty acids	267:286	volatile fatty acids	267:286	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	2	40	theme	H2/L	470:473	arg1	maximum					448:454	The maximum	444:454	The maximum of 23.10 mmoL H2/L	444:473	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	3	41	theme	fermentation	680:691	arg1	process					693:699	the fermentation process	676:699	the fermentation process	676:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	1	42	theme	fatty	276:280	arg1	acids					282:286	volatile fatty acids	267:286	volatile fatty acids	267:286	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	2	43	theme	7.0 g	518:522	arg1	SCB/L					524:528	7.0 g SCB/L	518:528	7.0 g SCB/L	518:528	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	3	44	dep	1.88 g/L	715:722	arg1	to					712:713	to	712:713	to	712:713	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	1	45	from	9.2 g/L	217:223	arg1	concentration					190:202	substrate concentration	180:202	substrate concentration (from 0.8 to 9.2 g/L)	180:224	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	45	from	9.2 g/L	217:223	arg1	pH					230:231	pH	230:231	pH (from 4.6 to 7.4)	230:249	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	45	from	9.2 g/L	217:223	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	46	theme	acids	282:286	arg1	production					288:297	hydrogen and volatile fatty acids production	254:297	hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	254:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	5	47	theme	optimized	1360:1368	arg1	condition					1370:1378	the optimized condition	1356:1378	the optimized condition (7.0 g SCB/L and pH 7.2)	1356:1403	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	3	48	dep	acids	652:656	arg1	1.65 g/L					741:748	1.65 g/L	741:748	1.65 g/L	741:748	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	48	dep	acids	652:656	arg1	1.88 g/L					715:722	1.88 g/L	715:722	1.88 g/L	715:722	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	48	dep	acids	652:656	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	49	theme	Butyric	595:601	arg1	acids					616:620	Butyric and succinic acids	595:620	Butyric and succinic acids	595:620	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	49	theme	Butyric	595:601	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	1	50	dep	7.4	246:248	arg1	to					243:244	to	243:244	to	243:244	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	51	from	effects	169:175	arg1	9.2 g/L					217:223	9.2 g/L	217:223	9.2 g/L	217:223	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	51	from	effects	169:175	arg1	production					288:297	hydrogen and volatile fatty acids production	254:297	hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	254:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	51	from	effects	169:175	arg1	7.4					246:248	7.4	246:248	7.4	246:248	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	3	52	theme	fatty	646:650	arg1	1.65 g/L					741:748	1.65 g/L	741:748	1.65 g/L	741:748	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	52	theme	fatty	646:650	arg1	VFA					659:661	VFA	659:661	VFA	659:661	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	52	theme	fatty	646:650	arg1	1.88 g/L					715:722	1.88 g/L	715:722	1.88 g/L	715:722	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	52	theme	fatty	646:650	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	52	theme	fatty	646:650	arg1	acids					616:620	Butyric and succinic acids	595:620	Butyric and succinic acids	595:620	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	5	53	theme	5.0 g	1224:1228	arg1	SCB/L					1230:1234	5.0 g SCB/L	1224:1234	5.0 g SCB/L	1224:1234	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	54	dep	condition	1370:1378	arg1	SCB/L					1387:1391	7.0 g SCB/L	1381:1391	7.0 g SCB/L	1381:1391	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	54	dep	condition	1370:1378	arg1	pH					1397:1398	pH 7.2	1397:1402	pH 7.2	1397:1402	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	2	55	theme	optimized	494:502	arg1	conditions					504:513	optimized conditions	494:513	optimized conditions of 7.0 g SCB/L and pH 7.2	494:539	The maximum of 23.10 mmoL H2/L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L).
30007891	1	56	theme	response	373:380	arg1	RSM					403:405	RSM	403:405	RSM	403:405	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	56	theme	response	373:380	arg1	methodology					390:400	a response surface methodology	371:400	a response surface methodology (RSM)	371:406	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	57	dep	9.2 g/L	217:223	arg1	to					214:215	to	214:215	to	214:215	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	4	58	theme	Paenibacillus	1011:1023	arg1	bacteria					1040:1047	Clostridium and Paenibacillus, autochthonous bacteria	995:1047	bacteria	1040:1047	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	5	59	theme	independent	1152:1162	arg1	variables					1164:1172	the independent variables	1148:1172	the independent variables	1148:1172	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	60	theme	central	1199:1205	arg1	condition					1213:1221	the central point condition	1195:1221	the central point condition (5.0 g SCB/L and pH 6.0)	1195:1246	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	1	61	theme	surface	382:388	arg1	RSM					403:405	RSM	403:405	RSM	403:405	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	61	theme	surface	382:388	arg1	methodology					390:400	a response surface methodology	371:400	a response surface methodology (RSM)	371:406	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	5	62	located	observed	1084:1091	arg2	changes					1071:1077	Significant changes	1059:1077	Significant changes	1059:1077	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	5	62	located	observed	1084:1091	arg1	community					1110:1118	the microbial community	1096:1118	the microbial community according to the changes in the independent variables	1096:1172	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30007891	0	63	with	productions	43:53	arg1	bacteria					92:99	autochthonous and allochthonous bacteria	60:99	autochthonous and allochthonous bacteria from sugarcane	60:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	1	64	theme	individual	147:156	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	1	65	theme	pH	230:231	arg1	effects					169:175	The individual and mutual effects	143:175	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB)	143:326	The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD).
30007891	0	66	theme	autochthonous	60:72	arg1	bacteria					92:99	autochthonous and allochthonous bacteria	60:99	autochthonous and allochthonous bacteria from sugarcane	60:114	Optimization of hydrogen and organic acids productions with autochthonous and allochthonous bacteria from sugarcane bagasse in batch reactors.
30007891	3	67	theme	succinic	607:614	arg1	acids					616:620	Butyric and succinic acids	595:620	Butyric and succinic acids	595:620	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	3	67	theme	succinic	607:614	arg1	acids					652:656	the major volatile fatty acids	627:656	the major volatile fatty acids (VFA) produced in the fermentation process	627:699	Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively).
30007891	4	68	theme	autochthonous	1026:1038	arg1	bacteria					1040:1047	Clostridium and Paenibacillus, autochthonous bacteria	995:1047	bacteria	1040:1047	According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB.
30007891	5	69	theme	microbial	1100:1108	arg1	community					1110:1118	the microbial community	1096:1118	the microbial community according to the changes in the independent variables	1096:1172	Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus, Escherichia and Clostridium, and Bacteroides and Enterobacter, which were identified in the optimized condition (7.0 g SCB/L and pH 7.2).
30821285	8	0	dep	in	1130:1131	arg1	vitro					1133:1137	vitro	1133:1137	vitro	1133:1137	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	10	1	theme	polymer	1289:1295	arg1	solution					1273:1280	solution	1273:1280	solution	1273:1280	solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C.
30821285	1	2	dep	Skin	81:84	arg1	substitutes					86:96	substitutes	86:96	substitutes	86:96	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	12	3	theme	polymer	1413:1419	arg1	solution					1397:1404	solution	1397:1404	solution of the polymer	1397:1419	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	13	4	theme	different	1526:1534	arg1	degree					1536:1541	a different degree	1524:1541	a different degree	1524:1541	All materials were biocompatible but to a different degree.
30821285	2	5	theme	good	321:324	arg1	matrix					326:331	a good matrix	319:331	a good matrix for cell adhesion and proliferation	319:367	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	2	5	theme	good	321:324	arg1	nanofibers					189:198	nanofibers	189:198	nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method	189:313	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	9	6	theme	stable	1183:1188	arg1	conditions					1190:1199	stable conditions	1183:1199	stable conditions for the formation of submicron PLA fibers	1183:1241	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	14	7	theme	layers	1686:1691	arg1	size					1673:1676	a maintained fiber size	1654:1676	a maintained fiber size of both layers	1654:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	1	8	dep	scaffolds	125:133	arg1	synthetic					147:155	synthetic	147:155	synthetic	147:155	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	8	dep	scaffolds	125:133	arg1	natural					136:142	natural	136:142	natural	136:142	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	14	9	theme	hydrophobic-hydrophilic	1610:1632	arg1	surface					1641:1647	a hydrophobic-hydrophilic domain surface	1608:1647	a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers	1608:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	10	10	theme	3:1	1313:1315	arg1	mixture					1317:1323	a 3:1 mixture	1311:1323	a 3:1 mixture of DCM	1311:1330	solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C.
30821285	14	11	theme	fiber	1667:1671	arg1	size					1673:1676	a maintained fiber size	1654:1676	a maintained fiber size of both layers	1654:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	8	12	theme	in	1130:1131	arg1	conditions					1139:1148	the in vitro conditions	1126:1148	the in vitro conditions	1126:1148	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	9	13	theme	13	1265:1266	arg1	%					1267:1267	%	1267:1267	%	1267:1267	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	3	14	dep	METHODS	370:376	arg1	determined					518:527	determined	518:527	were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions	513:676	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	14	15	theme	maintained	1656:1665	arg1	size					1673:1676	a maintained fiber size	1654:1676	a maintained fiber size of both layers	1654:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	2	16	theme	sodium	253:258	arg1	hyaluronate					260:270	sodium hyaluronate	253:270	sodium hyaluronate (HA)	253:275	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	2	16	theme	sodium	253:258	arg1	HA					273:274	HA	273:274	HA	273:274	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	9	17	theme	%	1267:1267	arg1	wt					1269:1270	a 13% wt	1263:1270	a 13% wt	1263:1270	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	3	18	theme	PLA	419:421	arg1	nanofibers					430:439	PLA and HA nanofibers	419:439	PLA and HA nanofibers	419:439	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	6	19	used	used	901:904	arg2	SEM					892:894	SEM	892:894	SEM	892:894	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	19	used	used	901:904	arg2	microscope					880:889	A scanning electron microscope	860:889	A scanning electron microscope (SEM)	860:895	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	20	theme	electron	871:878	arg1	microscope					880:889	A scanning electron microscope	860:889	A scanning electron microscope (SEM)	860:895	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	20	theme	electron	871:878	arg1	SEM					892:894	SEM	892:894	SEM	892:894	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	9	21	theme	PLA	1232:1234	arg1	fibers					1236:1241	submicron PLA fibers	1222:1241	submicron PLA fibers	1222:1241	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	1	22	theme	PURPOSE	73:79	arg1	scaffolds					125:133	scaffolds	125:133	scaffolds (natural or synthetic)	125:156	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	22	theme	PURPOSE	73:79	arg1	group					116:120	heterogeneous group	102:120	heterogeneous group of scaffolds (natural or synthetic) and cells	102:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	22	theme	PURPOSE	73:79	arg1	cells					162:166	cells	162:166	cells	162:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	22	theme	PURPOSE	73:79	arg1	Skin					81:84	PURPOSE Skin	73:84	PURPOSE Skin substitutes	73:96	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	23	theme	cells	162:166	arg1	scaffolds					125:133	scaffolds	125:133	scaffolds (natural or synthetic)	125:156	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	23	theme	cells	162:166	arg1	group					116:120	heterogeneous group	102:120	heterogeneous group of scaffolds (natural or synthetic) and cells	102:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	23	theme	cells	162:166	arg1	cells					162:166	cells	162:166	cells	162:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	23	theme	cells	162:166	arg1	Skin					81:84	PURPOSE Skin	73:84	PURPOSE Skin substitutes	73:96	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	6	24	theme	scanning	862:869	arg1	microscope					880:889	A scanning electron microscope	860:889	A scanning electron microscope (SEM)	860:895	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	24	theme	scanning	862:869	arg1	SEM					892:894	SEM	892:894	SEM	892:894	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	3	25	theme	electrospinning	400:414	arg1	conditions					386:395	Optimal conditions	378:395	Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens)	378:511	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	5	26	theme	spinning	790:797	arg1	solution					799:806	The spinning solution	786:806	The spinning solution	786:806	The spinning solution were characterized by surface tension and rheology.
30821285	10	27	from	°C	1342:1343	arg1	DMF					1332:1334	DMF	1332:1334	DMF at 45 °C	1332:1343	solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C.
30821285	6	28	dep	diameters	944:952	arg1	HA					963:964	HA	963:964	HA	963:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	28	dep	diameters	944:952	arg1	PLA					955:957	PLA	955:957	PLA	955:957	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	28	dep	diameters	944:952	arg1	diameters					944:952	the morphology and fiber diameters	919:952	the morphology and fiber diameters: PLA and HA	919:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	15	29	theme	biocompatibility	1728:1743	arg1	testing					1745:1751	biocompatibility testing	1728:1751	biocompatibility testing	1728:1751	The material positively underwent biocompatibility testing in contact with fibroblasts.
30821285	14	30	dep	CONCLUSIONS	1544:1554	arg1	laminate					1569:1576	laminate	1569:1576	laminate scaffold	1569:1585	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	0	31	theme	polylactide	18:28	arg1	fibers					45:50	polylactide and hyaluronic fibers	18:50	polylactide and hyaluronic fibers	18:50	The membrane with polylactide and hyaluronic fibers for skin substitute.
30821285	12	32	theme	alcohol	1475:1481	arg1	mixture					1440:1446	a 2:1 mixture	1434:1446	a 2:1 mixture of ammonia water and ethyl alcohol	1434:1481	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	10	33	theme	DCM	1328:1330	arg1	mixture					1317:1323	a 3:1 mixture	1311:1323	a 3:1 mixture of DCM	1311:1330	solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C.
30821285	14	34	with	surface	1641:1647	arg1	size					1673:1676	a maintained fiber size	1654:1676	a maintained fiber size of both layers	1654:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	6	35	theme	fiber	938:942	arg1	HA					963:964	HA	963:964	HA	963:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	35	theme	fiber	938:942	arg1	PLA					955:957	PLA	955:957	PLA	955:957	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	35	theme	fiber	938:942	arg1	diameters					944:952	the morphology and fiber diameters	919:952	the morphology and fiber diameters: PLA and HA	919:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	7	36	theme	nonwoven	991:998	arg1	Structure					967:975	Structure	967:975	Structure of the PLA/HA nonwoven	967:998	Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR).
30821285	3	37	theme	mixing	620:625	arg1	temperature					627:637	mixing temperature	620:637	mixing temperature	620:637	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	1	38	theme	heterogeneous	102:114	arg1	scaffolds					125:133	scaffolds	125:133	scaffolds (natural or synthetic)	125:156	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	38	theme	heterogeneous	102:114	arg1	group					116:120	heterogeneous group	102:120	heterogeneous group of scaffolds (natural or synthetic) and cells	102:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	38	theme	heterogeneous	102:114	arg1	cells					162:166	cells	162:166	cells	162:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	38	theme	heterogeneous	102:114	arg1	Skin					81:84	PURPOSE Skin	73:84	PURPOSE Skin substitutes	73:96	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	11	39	theme	hyaluronic	1350:1359	arg1	fibers					1361:1366	The hyaluronic fibers	1346:1366	The hyaluronic fibers	1346:1366	The hyaluronic fibers were prepared from a 12% wt.
30821285	0	40	theme	hyaluronic	34:43	arg1	fibers					45:50	polylactide and hyaluronic fibers	18:50	polylactide and hyaluronic fibers	18:50	The membrane with polylactide and hyaluronic fibers for skin substitute.
30821285	5	41	theme	surface	830:836	arg1	tension					838:844	surface tension	830:844	surface tension	830:844	The spinning solution were characterized by surface tension and rheology.
30821285	3	42	theme	appropriate	565:575	arg1	solvents					587:594	solvents	587:594	solvents	587:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	42	theme	appropriate	565:575	arg1	amount					577:582	the appropriate amount	561:582	the appropriate amount of solvents	561:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	43	theme	HA	427:428	arg1	nanofibers					430:439	PLA and HA nanofibers	419:439	PLA and HA nanofibers	419:439	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	6	44	theme	morphology	923:932	arg1	HA					963:964	HA	963:964	HA	963:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	44	theme	morphology	923:932	arg1	PLA					955:957	PLA	955:957	PLA	955:957	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	6	44	theme	morphology	923:932	arg1	diameters					944:952	the morphology and fiber diameters	919:952	the morphology and fiber diameters: PLA and HA	919:964	A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA.
30821285	14	45	theme	domain	1634:1639	arg1	surface					1641:1647	a hydrophobic-hydrophilic domain surface	1608:1647	a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers	1608:1691	CONCLUSIONS The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers.
30821285	3	46	theme	nanofibers	430:439	arg1	electrospinning					400:414	electrospinning	400:414	electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens)	400:511	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	12	47	theme	water	1459:1463	arg1	mixture					1440:1446	a 2:1 mixture	1434:1446	a 2:1 mixture of ammonia water and ethyl alcohol	1434:1481	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	12	48	theme	ammonia	1451:1457	arg1	water					1459:1463	ammonia water	1451:1463	ammonia water	1451:1463	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	11	49	theme	%	1391:1391	arg1	wt					1393:1394	a 12% wt	1387:1394	a 12% wt	1387:1394	The hyaluronic fibers were prepared from a 12% wt.
30821285	7	50	theme	PLA/HA	984:989	arg1	nonwoven					991:998	the PLA/HA nonwoven	980:998	the PLA/HA nonwoven	980:998	Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR).
30821285	3	51	theme	Optimal	378:384	arg1	conditions					386:395	Optimal conditions	378:395	Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens)	378:511	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	12	52	theme	ethyl	1469:1473	arg1	alcohol					1475:1481	ethyl alcohol	1469:1481	ethyl alcohol	1469:1481	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	8	53	theme	nonwoven	1068:1075	arg1	Biocompatibility					1044:1059	Biocompatibility	1044:1059	Biocompatibility of the nonwoven with fibroblasts (ECM producers)	1044:1108	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	3	54	theme	process	659:665	arg1	solvents					587:594	solvents	587:594	solvents	587:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	54	theme	process	659:665	arg1	amount					577:582	the appropriate amount	561:582	the appropriate amount of solvents	561:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	54	theme	process	659:665	arg1	conditions					667:676	electrospinning process conditions	643:676	electrospinning process conditions	643:676	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	2	55	theme	layer	205:209	arg1	composition					211:221	layer composition	205:221	layer composition made of polylactide (PLA) and sodium hyaluronate (HA)	205:275	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	0	56	with	membrane	4:11	arg1	fibers					45:50	polylactide and hyaluronic fibers	18:50	polylactide and hyaluronic fibers	18:50	The membrane with polylactide and hyaluronic fibers for skin substitute.
30821285	3	57	theme	PLA	473:475	arg1	compositions					459:470	layered compositions	451:470	layered compositions (PLA membrane covered with HA nonwovens)	451:511	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	57	theme	PLA	473:475	arg1	membrane					477:484	PLA membrane	473:484	PLA membrane covered with HA nonwovens	473:510	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	11	58	theme	12	1389:1390	arg1	%					1391:1391	%	1391:1391	%	1391:1391	The hyaluronic fibers were prepared from a 12% wt.
30821285	4	59	theme	fibers	778:783	arg1	properties					751:760	properties	751:760	properties	751:760	By changing the parameters, it was possible to control the diameter and properties of both polymer fibers.
30821285	4	59	theme	fibers	778:783	arg1	diameter					738:745	diameter	738:745	diameter	738:745	By changing the parameters, it was possible to control the diameter and properties of both polymer fibers.
30821285	4	60	dep	diameter	738:745	arg1	the					734:736	the	734:736	the	734:736	By changing the parameters, it was possible to control the diameter and properties of both polymer fibers.
30821285	15	61	with	contact	1756:1762	arg1	fibroblasts					1769:1779	fibroblasts	1769:1779	fibroblasts	1769:1779	The material positively underwent biocompatibility testing in contact with fibroblasts.
30821285	4	62	theme	polymer	770:776	arg1	fibers					778:783	both polymer fibers	765:783	both polymer fibers	765:783	By changing the parameters, it was possible to control the diameter and properties of both polymer fibers.
30821285	1	63	theme	scaffolds	125:133	arg1	scaffolds					125:133	scaffolds	125:133	scaffolds (natural or synthetic)	125:156	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	63	theme	scaffolds	125:133	arg1	group					116:120	heterogeneous group	102:120	heterogeneous group of scaffolds (natural or synthetic) and cells	102:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	63	theme	scaffolds	125:133	arg1	cells					162:166	cells	162:166	cells	162:166	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	1	63	theme	scaffolds	125:133	arg1	Skin					81:84	PURPOSE Skin	73:84	PURPOSE Skin substitutes	73:96	PURPOSE Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells.
30821285	9	64	dep	RESULTS	1151:1157	arg1	showed					1171:1176	showed	1171:1176	showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt	1171:1270	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	12	65	theme	2:1	1436:1438	arg1	mixture					1440:1446	a 2:1 mixture	1434:1446	a 2:1 mixture of ammonia water and ethyl alcohol	1434:1481	solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol.
30821285	9	66	theme	submicron	1222:1230	arg1	fibers					1236:1241	submicron PLA fibers	1222:1241	submicron PLA fibers	1222:1241	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	3	67	theme	polymer	597:603	arg1	concentration					605:617	polymer concentration	597:617	polymer concentration	597:617	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	2	68	theme	electrospinning	292:306	arg1	method					308:313	electrospinning method	292:313	electrospinning method	292:313	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	8	69	with	Biocompatibility	1044:1059	arg1	fibroblasts					1082:1092	fibroblasts	1082:1092	fibroblasts (ECM producers)	1082:1108	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	8	69	with	Biocompatibility	1044:1059	arg1	producers					1099:1107	ECM producers	1095:1107	ECM producers	1095:1107	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	3	70	theme	layered	451:457	arg1	compositions					459:470	layered compositions	451:470	layered compositions (PLA membrane covered with HA nonwovens)	451:511	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	70	theme	layered	451:457	arg1	membrane					477:484	PLA membrane	473:484	PLA membrane covered with HA nonwovens	473:510	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	71	theme	electrospinning	643:657	arg1	solvents					587:594	solvents	587:594	solvents	587:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	71	theme	electrospinning	643:657	arg1	amount					577:582	the appropriate amount	561:582	the appropriate amount of solvents	561:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	71	theme	electrospinning	643:657	arg1	conditions					667:676	electrospinning process conditions	643:676	electrospinning process conditions	643:676	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	2	72	theme	cell	337:340	arg1	adhesion					342:349	cell adhesion	337:349	cell adhesion	337:349	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	10	73	dep	DMF	1332:1334	arg1	solution					1273:1280	solution	1273:1280	solution	1273:1280	solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C.
30821285	2	74	with	nanofibers	189:198	arg1	composition					211:221	layer composition	205:221	layer composition made of polylactide (PLA) and sodium hyaluronate (HA)	205:275	We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation.
30821285	8	75	theme	ECM	1095:1097	arg1	fibroblasts					1082:1092	fibroblasts	1082:1092	fibroblasts (ECM producers)	1082:1108	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	8	75	theme	ECM	1095:1097	arg1	producers					1099:1107	ECM producers	1095:1107	ECM producers	1095:1107	Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions.
30821285	9	76	theme	fibers	1236:1241	arg1	formation					1209:1217	the formation	1205:1217	the formation of submicron PLA fibers	1205:1241	RESULTS The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt.
30821285	3	77	theme	solvents	587:594	arg1	solvents					587:594	solvents	587:594	solvents	587:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	77	theme	solvents	587:594	arg1	amount					577:582	the appropriate amount	561:582	the appropriate amount of solvents	561:594	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
30821285	3	78	theme	HA	499:500	arg1	nonwovens					502:510	HA nonwovens	499:510	HA nonwovens	499:510	METHODS Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions.
31374279	3	0	theme	Cellulose	339:347	arg1	CA					358:359	CA	358:359	CA	358:359	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	3	0	theme	Cellulose	339:347	arg1	acetate					349:355	Cellulose acetate	339:355	Cellulose acetate (CA) mats prepared by electrospinning technology	339:404	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	9	1	theme	considerable	1337:1348	arg1	prospects					1362:1370	considerable development prospects	1337:1370	considerable development prospects	1337:1370	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	7	2	theme	activity	1015:1022	arg1	analysis					989:996	The analysis	985:996	The analysis of antibacterial activity of the mats	985:1034	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	1	3	theme	products	191:198	arg1	research					117:124	research	117:124	research	117:124	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	1	3	theme	products	191:198	arg1	development					130:140	development	130:140	development	130:140	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	3	4	theme	acetate	349:355	arg1	mats					362:365	Cellulose acetate (CA) mats	339:365	Cellulose acetate (CA) mats prepared by electrospinning technology	339:404	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	6	5	theme	surface	918:924	arg1	characteristics					926:940	their surface characteristics	912:940	their surface characteristics	912:940	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	5	6	theme	three-dimensional	648:664	arg1	network					666:672	three-dimensional network	648:672	three-dimensional network	648:672	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	1	7	dep	research	117:124	arg1	The					113:115	The	113:115	The	113:115	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	8	8	theme	cellulose	1196:1204	arg1	mats					1206:1209	the LBL-structured cellulose mats	1177:1209	the LBL-structured cellulose mats	1177:1209	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	6	9	theme	angle	807:811	arg1	measurements					813:824	water contact angle measurements	793:824	water contact angle measurements	793:824	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	8	10	theme	LBL-structured	1181:1194	arg1	mats					1206:1209	the LBL-structured cellulose mats	1177:1209	the LBL-structured cellulose mats	1177:1209	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	8	11	contain	have	1211:1214	arg2	properties					1264:1273	mechanical properties	1253:1273	mechanical properties	1253:1273	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	8	11	contain	have	1211:1214	arg1	mats					1206:1209	the LBL-structured cellulose mats	1177:1209	the LBL-structured cellulose mats	1177:1209	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	8	11	contain	have	1211:1214	arg2	activity					1240:1247	excellent antibacterial activity	1216:1247	excellent antibacterial activity	1216:1247	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	5	12	theme	cellulose	599:607	arg1	mats					609:612	The cellulose mats	595:612	The cellulose mats	595:612	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	6	13	theme	properties	770:779	arg1	results					748:754	the results	744:754	the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats	744:853	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	4	14	theme	self-assembly	569:581	arg1	technology					583:592	layer-by-layer (LBL) self-assembly technology	548:592	layer-by-layer (LBL) self-assembly technology	548:592	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	15	theme	tannic	480:485	arg1	TA					493:494	TA	493:494	TA	493:494	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	15	theme	tannic	480:485	arg1	acid					487:490	tannic acid	480:490	tannic acid (TA)	480:495	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	16	used	used	502:505	arg2	TA					493:494	TA	493:494	TA	493:494	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	16	used	used	502:505	arg2	CS					472:473	CS	472:473	CS	472:473	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	16	used	used	502:505	arg2	Chitosan					462:469	Chitosan	462:469	Chitosan (CS)	462:474	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	4	16	used	used	502:505	arg2	acid					487:490	tannic acid	480:490	tannic acid (TA)	480:495	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	3	17	theme	alkali	443:448	arg1	hydrolysis					450:459	alkali hydrolysis	443:459	alkali hydrolysis	443:459	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	0	18	theme	nanofibrous	65:75	arg1	mats					77:80	cellulose nanofibrous mats	55:80	cellulose nanofibrous mats for antibacterial application	55:110	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	5	19	theme	good	624:627	arg1	structure					637:645	good fibrous structure	624:645	good fibrous structure	624:645	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	6	20	theme	water	793:797	arg1	measurements					813:824	water contact angle measurements	793:824	water contact angle measurements	793:824	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	8	21	theme	excellent	1216:1224	arg1	activity					1240:1247	excellent antibacterial activity	1216:1247	excellent antibacterial activity	1216:1247	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	0	22	theme	acid	16:19	arg1	bilayers					21:28	Chitosan/tannic acid bilayers	0:28	Chitosan/tannic acid bilayers	0:28	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	5	23	theme	fibrous	629:635	arg1	structure					637:645	good fibrous structure	624:645	good fibrous structure	624:645	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	2	24	used	used	290:293	arg2	materials					275:283	natural materials	267:283	natural materials	267:283	In this study, natural materials were used for producing a kind of antibacterial mats.
31374279	1	25	theme	important	214:222	arg1	topic					224:228	an important topic	211:228	an important topic of worldwide concern	211:249	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	0	26	theme	Chitosan/tannic	0:14	arg1	bilayers					21:28	Chitosan/tannic acid bilayers	0:28	Chitosan/tannic acid bilayers	0:28	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	9	27	theme	wound	1394:1398	arg1	dressing					1400:1407	wound dressing	1394:1407	wound dressing	1394:1407	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	2	28	theme	mats	333:336	arg1	kind					311:314	a kind	309:314	a kind of antibacterial mats	309:336	In this study, natural materials were used for producing a kind of antibacterial mats.
31374279	3	29	theme	electrospinning	379:393	arg1	technology					395:404	electrospinning technology	379:404	electrospinning technology	379:404	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	6	30	theme	contact	799:805	arg1	measurements					813:824	water contact angle measurements	793:824	water contact angle measurements	793:824	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	2	31	theme	antibacterial	319:331	arg1	mats					333:336	antibacterial mats	319:336	antibacterial mats	319:336	In this study, natural materials were used for producing a kind of antibacterial mats.
31374279	9	32	contain	have	1332:1335	arg1	mats					1308:1311	these nano-cellulose mats	1287:1311	these nano-cellulose mats	1287:1311	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	9	32	contain	have	1332:1335	arg2	prospects					1362:1370	considerable development prospects	1337:1370	considerable development prospects	1337:1370	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	1	33	theme	worldwide	233:241	arg1	concern					243:249	worldwide concern	233:249	worldwide concern	233:249	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	0	34	theme	cellulose	55:63	arg1	mats					77:80	cellulose nanofibrous mats	55:80	cellulose nanofibrous mats for antibacterial application	55:110	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	9	35	theme	food	1376:1379	arg1	packaging					1381:1389	food packaging	1376:1389	food packaging	1376:1389	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	6	36	theme	LBL	877:879	arg1	able					896:899	able	896:899	able	896:899	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	6	36	theme	LBL	877:879	arg1	technology					881:890	the LBL technology	873:890	the LBL technology	873:890	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	1	37	theme	concern	243:249	arg1	topic					224:228	an important topic	211:228	an important topic of worldwide concern	211:249	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	7	38	dep	99	1112:1113	arg1	to					1109:1110	to	1109:1110	to	1109:1110	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	8	39	theme	mechanical	1253:1262	arg1	properties					1264:1273	mechanical properties	1253:1273	mechanical properties	1253:1273	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	9	40	theme	nano-cellulose	1293:1306	arg1	mats					1308:1311	these nano-cellulose mats	1287:1311	these nano-cellulose mats	1287:1311	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	6	41	theme	mechanical	759:768	arg1	properties					770:779	mechanical properties	759:779	mechanical properties testing	759:787	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	8	42	theme	antibacterial	1226:1238	arg1	activity					1240:1247	excellent antibacterial activity	1216:1247	excellent antibacterial activity	1216:1247	Hence, the LBL-structured cellulose mats have excellent antibacterial activity and mechanical properties.
31374279	7	43	dep	%	1114:1114	arg1	99					1112:1113	99	1112:1113	99	1112:1113	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	6	44	theme	mechanical	962:971	arg1	properties					973:982	mechanical properties	962:982	mechanical properties	962:982	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	5	45	theme	small	678:682	arg1	diameter					698:705	small average fiber diameter	678:705	small average fiber diameter	678:705	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	5	46	dep	400 nm	727:732	arg1	to					724:725	to	724:725	to	724:725	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	3	47	theme	cellulose	424:432	arg1	mats					434:437	cellulose mats	424:437	cellulose mats	424:437	Cellulose acetate (CA) mats prepared by electrospinning technology were converted to cellulose mats via alkali hydrolysis.
31374279	5	48	theme	average	684:690	arg1	diameter					698:705	small average fiber diameter	678:705	small average fiber diameter	678:705	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	7	49	theme	antibacterial	1116:1128	arg1	activity					1130:1137	up to 99% antibacterial activity	1106:1137	up to 99% antibacterial activity against Staphylococcus aureus	1106:1167	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	7	50	theme	mats	1031:1034	arg1	activity					1015:1022	antibacterial activity	1001:1022	antibacterial activity of the mats	1001:1034	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	1	51	theme	environmentally	145:159	arg1	friendly					161:168	environmentally friendly	145:168	environmentally friendly	145:168	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	4	52	theme	composite	524:532	arg1	mats					534:537	the composite mats	520:537	the composite mats	520:537	Chitosan (CS) and tannic acid (TA) were used to fabricate the composite mats by using layer-by-layer (LBL) self-assembly technology.
31374279	5	53	theme	fiber	692:696	arg1	diameter					698:705	small average fiber diameter	678:705	small average fiber diameter	678:705	The cellulose mats exhibited good fibrous structure, three-dimensional network and small average fiber diameter ranging from 300 to 400 nm.
31374279	6	54	theme	mats	850:853	arg1	properties					770:779	mechanical properties	759:779	mechanical properties testing	759:787	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	6	54	theme	mats	850:853	arg1	measurements					813:824	water contact angle measurements	793:824	water contact angle measurements	793:824	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	0	55	dep	layer-by-layer	30:43	arg1	deposited					45:53	deposited	45:53	layer-by-layer deposited cellulose nanofibrous mats for antibacterial application	30:110	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	6	56	theme	measurements	813:824	arg1	results					748:754	the results	744:754	the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats	744:853	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	1	57	theme	friendly	161:168	arg1	research					117:124	research	117:124	research	117:124	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	1	57	theme	friendly	161:168	arg1	development					130:140	development	130:140	development	130:140	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	7	58	theme	antibacterial	1054:1066	arg1	activity					1068:1075	antibacterial activity	1054:1075	antibacterial activity against Escherichia coli	1054:1100	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	6	59	theme	LBL-structured	835:848	arg1	mats					850:853	these LBL-structured mats	829:853	these LBL-structured mats	829:853	Besides, the results of mechanical properties testing and water contact angle measurements of these LBL-structured mats demonstrated that the LBL technology was able to improve their surface characteristics, hydrophilicity and mechanical properties.
31374279	0	60	theme	antibacterial	86:98	arg1	application					100:110	antibacterial application	86:110	antibacterial application	86:110	Chitosan/tannic acid bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
31374279	1	61	theme	nontoxic	174:181	arg1	products					191:198	nontoxic biomass products	174:198	nontoxic biomass products	174:198	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	9	62	theme	development	1350:1360	arg1	prospects					1362:1370	considerable development prospects	1337:1370	considerable development prospects	1337:1370	Therefore, these nano-cellulose mats can be expected to have considerable development prospects for food packaging or wound dressing.
31374279	2	63	theme	natural	267:273	arg1	materials					275:283	natural materials	267:283	natural materials	267:283	In this study, natural materials were used for producing a kind of antibacterial mats.
31374279	7	64	theme	antibacterial	1001:1013	arg1	activity					1015:1022	antibacterial activity	1001:1022	antibacterial activity of the mats	1001:1034	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31374279	1	65	theme	biomass	183:189	arg1	products					191:198	nontoxic biomass products	174:198	nontoxic biomass products	174:198	The research and development of environmentally friendly and nontoxic biomass products has become an important topic of worldwide concern.
31374279	7	66	dep	activity	1130:1137	arg1	%					1114:1114	%	1114:1114	%	1114:1114	The analysis of antibacterial activity of the mats revealed over 86% antibacterial activity against Escherichia coli and up to 99% antibacterial activity against Staphylococcus aureus.
31581521	2	0	theme	attributes	523:532	arg1	low-abundance					478:490	low-abundance	478:490	low-abundance	478:490	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	3	1	theme	specific	786:793	arg1	variants					812:819	specific Fc glycosylation variants	786:819	specific Fc glycosylation variants produced by in vitro glyco-engineering technology	786:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	3	2	theme	conformational	760:773	arg1	changes					775:781	conformational changes	760:781	conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology	760:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	1	3	theme	exchange-mass	164:176	arg1	H/DX-MS					192:198	H/DX-MS	192:198	H/DX-MS	192:198	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	1	3	theme	exchange-mass	164:176	arg1	spectrometry					178:189	hydrogen/deuterium exchange-mass spectrometry	145:189	hydrogen/deuterium exchange-mass spectrometry (H/DX-MS)	145:199	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	1	4	theme	antibody	271:278	arg1	modifications					280:292	chemical and post-translational antibody modifications	239:292	chemical and post-translational antibody modifications	239:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	0	5	theme	H/D	65:67	arg1	Exchange					69:76	Backbone Amide H/D Exchange	50:76	Backbone Amide H/D Exchange	50:76	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	0	6	from	Impact	4:9	arg1	Exchange					69:76	Backbone Amide H/D Exchange	50:76	Backbone Amide H/D Exchange	50:76	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	2	7	from	assessment	368:377	arg1	development					431:441	development	431:441	development	431:441	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	2	7	from	assessment	368:377	arg1	research					418:425	biopharmaceutical research	400:425	biopharmaceutical research	400:425	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	5	8	theme	sensitive	1194:1202	arg1	levels					1204:1209	sensitive levels	1194:1209	sensitive levels	1194:1209	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	1	9	theme	modifications	280:292	arg1	analyses					227:234	the structural impact analyses	205:234	the structural impact analyses of chemical and post-translational antibody modifications	205:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	2	10	theme	modifications	563:575	arg1	effects					552:558	overlapping effects	540:558	overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation	540:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	3	11	theme	H/DX-MS	731:737	arg1	technique					739:747	the H/DX-MS technique	727:747	the H/DX-MS technique	727:747	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	4	12	theme	larger	941:946	arg1	structures					955:964	larger glycan structures	941:964	larger glycan structures	941:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	2	13	theme	acid	621:624	arg1	modifications					626:638	chemical amino acid modifications	606:638	chemical amino acid modifications; e.g., oxidation or deamidation	606:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	5	14	theme	corresponding	1044:1056	arg1	properties					1066:1075	corresponding binding properties	1044:1075	corresponding binding properties	1044:1075	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	2	15	theme	protein	383:389	arg1	drugs					391:395	protein drugs	383:395	protein drugs	383:395	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	4	16	theme	Fc	901:902	arg1	segments					915:922	Fc Cγ2 domain segments	901:922	Fc Cγ2 domain segments correlating with larger glycan structures	901:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	5	17	theme	binding	1058:1064	arg1	properties					1066:1075	corresponding binding properties	1044:1075	corresponding binding properties	1044:1075	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	2	18	theme	overlapping	540:550	arg1	effects					552:558	overlapping effects	540:558	overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation	540:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	3	19	dep	in	833:834	arg1	vitro					836:840	vitro	836:840	vitro	836:840	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	2	20	theme	critical	506:513	arg1	attributes					523:532	critical quality attributes	506:532	critical quality attributes	506:532	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	2	21	with	effects	552:558	arg1	modifications					626:638	chemical amino acid modifications	606:638	chemical amino acid modifications; e.g., oxidation or deamidation	606:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	2	22	dep	oxidation	647:655	arg1	e.g.					641:644	e.g.	641:644	e.g.	641:644	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	2	23	theme	quality	515:521	arg1	attributes					523:532	critical quality attributes	506:532	critical quality attributes	506:532	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	4	24	theme	domain	908:913	arg1	segments					915:922	Fc Cγ2 domain segments	901:922	Fc Cγ2 domain segments correlating with larger glycan structures	901:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	5	25	theme	significant	999:1009	arg1	differences					1028:1038	significant deuterium uptake differences	999:1038	significant deuterium uptake differences	999:1038	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	4	26	theme	glycan	948:953	arg1	structures					955:964	larger glycan structures	941:964	larger glycan structures	941:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	3	27	theme	glycosylation	798:810	arg1	variants					812:819	specific Fc glycosylation variants	786:819	specific Fc glycosylation variants produced by in vitro glyco-engineering technology	786:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	4	28	theme	Cγ2	904:906	arg1	segments					915:922	Fc Cγ2 domain segments	901:922	Fc Cγ2 domain segments correlating with larger glycan structures	901:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	5	29	theme	deuterium	1011:1019	arg1	differences					1028:1038	significant deuterium uptake differences	999:1038	significant deuterium uptake differences	999:1038	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	5	30	dep	monitored	1097:1105	arg1	as					1094:1095	as	1094:1095	as	1094:1095	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	0	31	theme	Immunoglobulin	14:27	arg1	Sialylation					35:45	Immunoglobulin G1 Fc Sialylation	14:45	Immunoglobulin G1 Fc Sialylation	14:45	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	2	32	theme	biopharmaceutical	400:416	arg1	research					418:425	biopharmaceutical research	400:425	biopharmaceutical research	400:425	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	5	33	theme	uptake	1021:1026	arg1	differences					1028:1038	significant deuterium uptake differences	999:1038	significant deuterium uptake differences	999:1038	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	5	34	dep	receptors	1083:1091	arg1	monitored					1097:1105	monitored	1097:1105	monitored by SPR	1097:1112	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	1	35	theme	various	319:325	arg1	studies					327:333	various studies	319:333	various studies	319:333	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	5	36	theme	α-2,6-sialylated	1134:1149	arg1	variants					1168:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	1	37	theme	structural	209:218	arg1	analyses					227:234	the structural impact analyses	205:234	the structural impact analyses of chemical and post-translational antibody modifications	205:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	0	38	theme	Fc	32:33	arg1	Sialylation					35:45	Immunoglobulin G1 Fc Sialylation	14:45	Immunoglobulin G1 Fc Sialylation	14:45	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	3	39	theme	Fc	795:796	arg1	variants					812:819	specific Fc glycosylation variants	786:819	specific Fc glycosylation variants produced by in vitro glyco-engineering technology	786:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	5	40	theme	Fc	1151:1152	arg1	variants					1168:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	1	41	theme	impact	220:225	arg1	analyses					227:234	the structural impact analyses	205:234	the structural impact analyses of chemical and post-translational antibody modifications	205:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	0	42	theme	G1	29:30	arg1	Sialylation					35:45	Immunoglobulin G1 Fc Sialylation	14:45	Immunoglobulin G1 Fc Sialylation	14:45	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	3	43	theme	in	833:834	arg1	technology					860:869	in vitro glyco-engineering technology	833:869	in vitro glyco-engineering technology	833:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	5	44	theme	glycosylation	1154:1166	arg1	variants					1168:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	2	45	theme	amino	615:619	arg1	modifications					626:638	chemical amino acid modifications	606:638	chemical amino acid modifications; e.g., oxidation or deamidation	606:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	3	46	theme	variants	812:819	arg1	changes					775:781	conformational changes	760:781	conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology	760:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	1	47	theme	higher-order	97:108	arg1	information					121:131	higher-order structural information	97:131	higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications	97:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	0	48	theme	Sialylation	35:45	arg1	Impact					4:9	The Impact	0:9	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.	0:77	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	2	49	theme	chemical	606:613	arg1	modifications					626:638	chemical amino acid modifications	606:638	chemical amino acid modifications; e.g., oxidation or deamidation	606:670	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	4	50	theme	less	888:891	arg1	H/DX					893:896	less H/DX	888:896	less H/DX	888:896	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	1	51	theme	structural	110:119	arg1	information					121:131	higher-order structural information	97:131	higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications	97:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	4	52	from	trend	874:878	arg1	segments					915:922	Fc Cγ2 domain segments	901:922	Fc Cγ2 domain segments correlating with larger glycan structures	901:964	A trend towards less H/DX in Fc Cγ2 domain segments correlating with larger glycan structures could be confirmed.
31581521	1	53	theme	information	121:131	arg1	usefulness					83:92	The usefulness	79:92	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications	79:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	5	54	gly	α-2,6-sialylated	1134:1149	arg1	variants					1168:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	5	55	theme	α-2,3-	1123:1128	arg1	variants					1168:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	α-2,3- and α-2,6-sialylated Fc glycosylation variants	1123:1175	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	5	56	theme	Fc	1080:1081	arg1	receptors					1083:1091	Fc receptors	1080:1091	Fc receptors (as monitored by SPR)	1080:1113	Furthermore, significant deuterium uptake differences and corresponding binding properties to Fc receptors (as monitored by SPR) between α-2,3- and α-2,6-sialylated Fc glycosylation variants were verified at sensitive levels.
31581521	2	57	theme	structure-function	349:366	arg1	assessment					368:377	the structure-function assessment	345:377	the structure-function assessment for protein drugs in biopharmaceutical research and development	345:441	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	2	57	theme	structure-function	349:366	arg1	impeded					452:458	impeded	452:458	impeded	452:458	However, the structure-function assessment for protein drugs in biopharmaceutical research and development is often impeded by the relatively low-abundance (below 5%) of critical quality attributes or by overlapping effects of modifications, such as glycosylation, with chemical amino acid modifications; e.g., oxidation or deamidation.
31581521	1	58	theme	chemical	239:246	arg1	modifications					280:292	chemical and post-translational antibody modifications	239:292	chemical and post-translational antibody modifications	239:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	0	59	theme	Amide	59:63	arg1	Exchange					69:76	Backbone Amide H/D Exchange	50:76	Backbone Amide H/D Exchange	50:76	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	3	60	theme	glyco-engineering	842:858	arg1	technology					860:869	in vitro glyco-engineering technology	833:869	in vitro glyco-engineering technology	833:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	0	61	theme	Backbone	50:57	arg1	Exchange					69:76	Backbone Amide H/D Exchange	50:76	Backbone Amide H/D Exchange	50:76	The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange.
31581521	3	62	theme	technique	739:747	arg1	applicability					710:722	the applicability	706:722	the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology	706:869	We present results demonstrating the applicability of the H/DX-MS technique to monitor conformational changes of specific Fc glycosylation variants produced by in vitro glyco-engineering technology.
31581521	1	63	theme	hydrogen/deuterium	145:162	arg1	H/DX-MS					192:198	H/DX-MS	192:198	H/DX-MS	192:198	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	1	63	theme	hydrogen/deuterium	145:162	arg1	spectrometry					178:189	hydrogen/deuterium exchange-mass spectrometry	145:189	hydrogen/deuterium exchange-mass spectrometry (H/DX-MS)	145:199	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31581521	1	64	theme	post-translational	252:269	arg1	modifications					280:292	chemical and post-translational antibody modifications	239:292	chemical and post-translational antibody modifications	239:292	The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in various studies.
31207987	5	0	theme	extraction	270:279	arg1	methods					318:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	8	1	theme	amylose	646:652	arg1	contents					669:676	more amylose and phosphorus contents	641:676	more amylose and phosphorus contents	641:676	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	13	2	theme	pasting	1300:1306	arg1	temperature					1308:1318	pasting temperature	1300:1318	pasting temperature	1300:1318	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	15	3	theme	potential	1419:1427	arg1	alternatives					1443:1454	potential technological alternatives	1419:1454	potential technological alternatives	1419:1454	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	7	4	theme	starch	466:471	arg1	oval					487:490	oval	487:490	oval	487:490	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	7	4	theme	starch	466:471	arg1	granules					473:480	The starch granules	462:480	The starch granules	462:480	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	8	5	theme	average	702:708	arg1	size					718:721	smaller average granule size	694:721	smaller average granule size	694:721	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	0	6	theme	opposita	127:134	arg1	Thunb					136:140	Dioscorea opposita Thunb	117:140	Dioscorea opposita Thunb	117:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	9	7	with	°C	843:844	arg1	enthalpy					854:861	an enthalpy	851:861	an enthalpy of 2.0 J/g	851:872	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	9	8	theme	scanning	737:744	arg1	DSC					759:761	DSC	759:761	DSC	759:761	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	9	8	theme	scanning	737:744	arg1	calorimetry					746:756	Differential scanning calorimetry	724:756	Differential scanning calorimetry (DSC)	724:762	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	0	9	from	Characteristics	67:81	arg1	Thunb					136:140	Dioscorea opposita Thunb	117:140	Dioscorea opposita Thunb	117:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	13	10	theme	lowest	1255:1260	arg1	trough					1262:1267	the lowest trough	1251:1267	the lowest trough	1251:1267	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	5	11	theme	alkaline	292:299	arg1	ABS					313:315	ABS	313:315	ABS	313:315	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	5	11	theme	alkaline	292:299	arg1	extraction					301:310	alkaline extraction	292:310	alkaline extraction (ABS)	292:316	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	12	12	with	comparison	1129:1138	arg1	EBS					1145:1147	EBS	1145:1147	EBS	1145:1147	Additionally, ABS presented greater clarity in comparison with EBS and SBS.
31207987	12	12	with	comparison	1129:1138	arg1	SBS					1153:1155	SBS	1153:1155	SBS	1153:1155	Additionally, ABS presented greater clarity in comparison with EBS and SBS.
31207987	8	13	contain	having	599:604	arg2	size					629:632	larger average granule size	606:632	larger average granule size	606:632	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	8	13	contain	having	599:604	arg1	starches					590:597	The starches	586:597	The starches having larger average granule size	586:632	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	8	14	theme	phosphorus	658:667	arg1	contents					669:676	more amylose and phosphorus contents	641:676	more amylose and phosphorus contents	641:676	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	13	15	theme	highest	1204:1210	arg1	viscosity					1217:1225	peak viscosity	1212:1225	peak viscosity	1212:1225	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	10	16	theme	°C	980:981	arg1	regions					929:935	the regions	925:935	the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively	925:995	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	0	17	from	Influences	0:9	arg1	Characteristics					67:81	Physicochemical and Functional Characteristics	36:81	Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb	36:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	0	18	theme	Bulbil	96:101	arg1	Starches					103:110	Three New Bulbil Starches	86:110	Three New Bulbil Starches from Dioscorea opposita Thunb	86:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	7	19	theme	B-type	569:574	arg1	patterns					576:583	A-type and B-type patterns	558:583	A-type and B-type patterns	558:583	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	10	20	dep	°C	956:957	arg1	to					948:949	to	948:949	to	948:949	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	7	21	theme	patterns	576:583	arg1	type					537:540	its crystal type	525:540	its crystal type	525:540	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	7	21	theme	patterns	576:583	arg1	mixture					547:553	a mixture	545:553	a mixture of A-type and B-type patterns	545:583	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	7	22	theme	A-type	558:563	arg1	patterns					576:583	A-type and B-type patterns	558:583	A-type and B-type patterns	558:583	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	14	23	theme	frozen	1379:1384	arg1	EBS					1340:1342	EBS	1340:1342	EBS	1340:1342	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	23	theme	frozen	1379:1384	arg1	ABS					1332:1334	ABS	1332:1334	ABS	1332:1334	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	23	theme	frozen	1379:1384	arg1	additives					1391:1399	frozen food additives	1379:1399	frozen food additives	1379:1399	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	9	24	theme	°C	832:833	arg1	°C					843:844	65.8 °C to 76.3 °C	827:844	65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g	827:872	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	3	25	theme	opposita	195:202	arg1	Thunb					204:208	Dioscoreae opposita Thunb	184:208	Dioscoreae opposita Thunb	184:208	Starches from the bulbils of Dioscoreae opposita Thunb.
31207987	0	26	theme	Extraction	14:23	arg1	Methods					25:31	Extraction Methods	14:31	Extraction Methods	14:31	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	10	27	theme	endothermic	879:889	arg1	transitions					891:901	The endothermic transitions	875:901	The endothermic transitions of ABS and EBS	875:916	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	5	28	theme	aqueous	239:245	arg1	SBS					257:259	SBS	257:259	SBS	257:259	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	5	28	theme	aqueous	239:245	arg1	steeping					247:254	aqueous steeping	239:254	aqueous steeping (SBS)	239:260	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	7	29	theme	crystal	529:535	arg1	type					537:540	its crystal type	525:540	its crystal type	525:540	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	7	29	theme	crystal	529:535	arg1	mixture					547:553	a mixture	545:553	a mixture of A-type and B-type patterns	545:583	The starch granules were oval, spherical and kidney-shaped and its crystal type is a mixture of A-type and B-type patterns.
31207987	15	30	theme	applications	1533:1544	arg1	preservation					1467:1478	quality preservation	1459:1478	quality preservation of frozen starch-based products and other industrial applications	1459:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	13	31	dep	viscosity	1217:1225	arg1	the					1200:1202	the	1200:1202	the	1200:1202	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	11	32	theme	ABS	1032:1034	arg1	J/g					1063:1065	13.8 and 11.5 J/g	1049:1065	13.8 and 11.5 J/g	1049:1065	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	11	32	theme	ABS	1032:1034	arg1	enthalpies					1018:1027	The gelationization enthalpies	998:1027	The gelationization enthalpies of ABS and EBS	998:1042	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	14	33	theme	food	1361:1364	arg1	EBS					1340:1342	EBS	1340:1342	EBS	1340:1342	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	33	theme	food	1361:1364	arg1	thickener					1366:1374	food thickener	1361:1374	food thickener	1361:1374	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	33	theme	food	1361:1364	arg1	ABS					1332:1334	ABS	1332:1334	ABS	1332:1334	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	13	34	theme	Pasting	1158:1164	arg1	properties					1166:1175	Pasting properties	1158:1175	Pasting properties	1158:1175	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	11	35	theme	EBS	1040:1042	arg1	J/g					1063:1065	13.8 and 11.5 J/g	1049:1065	13.8 and 11.5 J/g	1049:1065	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	11	35	theme	EBS	1040:1042	arg1	enthalpies					1018:1027	The gelationization enthalpies	998:1027	The gelationization enthalpies of ABS and EBS	998:1042	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	8	36	theme	average	613:619	arg1	size					629:632	larger average granule size	606:632	larger average granule size	606:632	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	15	37	theme	products	1503:1510	arg1	preservation					1467:1478	quality preservation	1459:1478	quality preservation of frozen starch-based products and other industrial applications	1459:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	9	38	dep	°C	843:844	arg1	to					835:836	to	835:836	to	835:836	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	5	39	theme	enzyme	263:268	arg1	extraction					270:279	enzyme extraction	263:279	enzyme extraction (EBS)	263:285	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	5	39	theme	enzyme	263:268	arg1	EBS					282:284	EBS	282:284	EBS	282:284	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	12	40	theme	greater	1110:1116	arg1	clarity					1118:1124	greater clarity	1110:1124	greater clarity	1110:1124	Additionally, ABS presented greater clarity in comparison with EBS and SBS.
31207987	15	41	theme	frozen	1483:1488	arg1	products					1503:1510	frozen starch-based products	1483:1510	frozen starch-based products	1483:1510	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	8	42	theme	more	641:644	arg1	contents					669:676	more amylose and phosphorus contents	641:676	more amylose and phosphorus contents	641:676	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	6	43	theme	physicochemical	345:359	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	15	44	theme	technological	1429:1441	arg1	alternatives					1443:1454	potential technological alternatives	1419:1454	potential technological alternatives	1419:1454	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	13	45	contain	had	1196:1198	arg1	ABS					1192:1194	ABS	1192:1194	ABS	1192:1194	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	viscosity					1276:1284	final viscosity	1270:1284	final viscosity	1270:1284	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	temperature					1308:1318	pasting temperature	1300:1318	pasting temperature	1300:1318	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	viscosity					1217:1225	peak viscosity	1212:1225	peak viscosity	1212:1225	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	breakdown					1228:1236	breakdown	1228:1236	breakdown	1228:1236	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	SBS					1243:1245	SBS	1243:1245	SBS had the lowest trough	1243:1267	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	13	45	contain	had	1196:1198	arg2	setback					1287:1293	setback	1287:1293	setback	1287:1293	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	9	46	contain	had	784:786	arg1	SBS					780:782	the SBS	776:782	the SBS	776:782	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	9	46	contain	had	784:786	arg2	transition					803:812	an endothermic transition	788:812	an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g	788:872	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	5	47	theme	extraction	301:310	arg1	methods					318:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	13	48	theme	final	1270:1274	arg1	viscosity					1276:1284	final viscosity	1270:1284	final viscosity	1270:1284	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	11	49	theme	gelationization	1002:1016	arg1	J/g					1063:1065	13.8 and 11.5 J/g	1049:1065	13.8 and 11.5 J/g	1049:1065	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	11	49	theme	gelationization	1002:1016	arg1	enthalpies					1018:1027	The gelationization enthalpies	998:1027	The gelationization enthalpies of ABS and EBS	998:1042	The gelationization enthalpies of ABS and EBS were 13.8 and 11.5 J/g, respectively.
31207987	9	50	theme	Differential	724:735	arg1	DSC					759:761	DSC	759:761	DSC	759:761	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	9	50	theme	Differential	724:735	arg1	calorimetry					746:756	Differential scanning calorimetry	724:756	Differential scanning calorimetry (DSC)	724:762	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	15	51	from	alternatives	1443:1454	arg1	preservation					1467:1478	quality preservation	1459:1478	quality preservation of frozen starch-based products and other industrial applications	1459:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	8	52	theme	smaller	694:700	arg1	size					718:721	smaller average granule size	694:721	smaller average granule size	694:721	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	10	53	theme	°C	956:957	arg1	regions					929:935	the regions	925:935	the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively	925:995	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	10	54	dep	°C	980:981	arg1	to					972:973	to	972:973	to	972:973	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	8	55	theme	granule	710:716	arg1	size					718:721	smaller average granule size	694:721	smaller average granule size	694:721	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	10	56	theme	°C	969:970	arg1	°C					980:981	66.8 °C to 82.0 °C	964:981	66.8 °C to 82.0 °C	964:981	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	15	57	theme	other	1516:1520	arg1	applications					1533:1544	other industrial applications	1516:1544	other industrial applications	1516:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	0	58	theme	New	92:94	arg1	Starches					103:110	Three New Bulbil Starches	86:110	Three New Bulbil Starches from Dioscorea opposita Thunb	86:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	6	59	theme	starches	434:441	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	3	60	from	bulbils	173:179	arg1	Starches					155:162	Starches	155:162	Starches from the bulbils of Dioscoreae opposita Thunb.	155:209	Starches from the bulbils of Dioscoreae opposita Thunb.
31207987	9	61	theme	endothermic	791:801	arg1	transition					803:812	an endothermic transition	788:812	an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g	788:872	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	13	62	theme	peak	1212:1215	arg1	viscosity					1217:1225	peak viscosity	1212:1225	peak viscosity	1212:1225	Pasting properties indicated that ABS had the highest peak viscosity, breakdown, but SBS had the lowest trough, final viscosity, setback, and pasting temperature.
31207987	0	63	theme	Starches	103:110	arg1	Characteristics					67:81	Physicochemical and Functional Characteristics	36:81	Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb	36:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	6	64	theme	morphological	395:407	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	0	65	theme	Dioscorea	117:125	arg1	Thunb					136:140	Dioscorea opposita Thunb	117:140	Dioscorea opposita Thunb	117:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	6	66	theme	thermal	383:389	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	3	67	theme	Thunb	204:208	arg1	bulbils					173:179	the bulbils	169:179	the bulbils of Dioscoreae opposita Thunb	169:208	Starches from the bulbils of Dioscoreae opposita Thunb.
31207987	10	68	theme	ABS	906:908	arg1	transitions					891:901	The endothermic transitions	875:901	The endothermic transitions of ABS and EBS	875:916	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	6	69	theme	composition	370:380	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	3	70	theme	Dioscoreae	184:193	arg1	Thunb					204:208	Dioscoreae opposita Thunb	184:208	Dioscoreae opposita Thunb	184:208	Starches from the bulbils of Dioscoreae opposita Thunb.
31207987	0	71	theme	Methods	25:31	arg1	Influences					0:9	Influences	0:9	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.	0:141	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	0	72	theme	Physicochemical	36:50	arg1	Characteristics					67:81	Physicochemical and Functional Characteristics	36:81	Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb	36:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	6	73	theme	mineral	362:368	arg1	characteristics					409:423	The physicochemical, mineral composition, thermal and morphological characteristics	341:423	characteristics	409:423	The physicochemical, mineral composition, thermal and morphological characteristics of these starches were investigated.
31207987	15	74	theme	industrial	1522:1531	arg1	applications					1533:1544	other industrial applications	1516:1544	other industrial applications	1516:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	0	75	theme	Functional	56:65	arg1	Characteristics					67:81	Physicochemical and Functional Characteristics	36:81	Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb	36:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	5	76	theme	steeping	247:254	arg1	methods					318:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods	239:324	Tiegun were isolated by aqueous steeping (SBS), enzyme extraction (EBS), and alkaline extraction (ABS) methods, respectively.
31207987	10	77	theme	EBS	914:916	arg1	transitions					891:901	The endothermic transitions	875:901	The endothermic transitions of ABS and EBS	875:916	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	8	78	theme	larger	606:611	arg1	size					629:632	larger average granule size	606:632	larger average granule size	606:632	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	14	79	theme	food	1386:1389	arg1	EBS					1340:1342	EBS	1340:1342	EBS	1340:1342	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	79	theme	food	1386:1389	arg1	ABS					1332:1334	ABS	1332:1334	ABS	1332:1334	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	79	theme	food	1386:1389	arg1	additives					1391:1399	frozen food additives	1379:1399	frozen food additives	1379:1399	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	10	80	theme	°C	945:946	arg1	°C					956:957	67.9 °C to 73.0 °C	940:957	67.9 °C to 73.0 °C	940:957	The endothermic transitions of ABS and EBS showed the regions of 67.9 °C to 73.0 °C, and 66.8 °C to 82.0 °C, respectively.
31207987	12	81	attach	presented	1100:1108	arg2	ABS					1096:1098	ABS	1096:1098	ABS	1096:1098	Additionally, ABS presented greater clarity in comparison with EBS and SBS.
31207987	12	81	attach	presented	1100:1108	arg1	comparison					1129:1138	comparison	1129:1138	comparison with EBS and SBS	1129:1155	Additionally, ABS presented greater clarity in comparison with EBS and SBS.
31207987	8	82	theme	granule	621:627	arg1	size					629:632	larger average granule size	606:632	larger average granule size	606:632	The starches having larger average granule size showed more amylose and phosphorus contents than those with smaller average granule size.
31207987	15	83	theme	starch-based	1490:1501	arg1	products					1503:1510	frozen starch-based products	1483:1510	frozen starch-based products	1483:1510	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	0	84	from	Thunb	136:140	arg1	Starches					103:110	Three New Bulbil Starches	86:110	Three New Bulbil Starches from Dioscorea opposita Thunb	86:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	0	84	from	Thunb	136:140	arg1	Characteristics					67:81	Physicochemical and Functional Characteristics	36:81	Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb	36:140	Influences of Extraction Methods on Physicochemical and Functional Characteristics of Three New Bulbil Starches from Dioscorea opposita Thunb.
31207987	9	85	theme	J/g	870:872	arg1	enthalpy					854:861	an enthalpy	851:861	an enthalpy of 2.0 J/g	851:872	Differential scanning calorimetry (DSC) showed that the SBS had an endothermic transition ranging from 65.8 °C to 76.3 °C with an enthalpy of 2.0 J/g.
31207987	15	86	theme	quality	1459:1465	arg1	preservation					1467:1478	quality preservation	1459:1478	quality preservation of frozen starch-based products and other industrial applications	1459:1544	SBS and EBS were potential technological alternatives in quality preservation of frozen starch-based products and other industrial applications.
31207987	14	87	used	used	1353:1356	arg2	ABS					1332:1334	ABS	1332:1334	ABS	1332:1334	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	87	used	used	1353:1356	arg2	thickener					1366:1374	food thickener	1361:1374	food thickener	1361:1374	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	87	used	used	1353:1356	arg2	additives					1391:1399	frozen food additives	1379:1399	frozen food additives	1379:1399	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31207987	14	87	used	used	1353:1356	arg2	EBS					1340:1342	EBS	1340:1342	EBS	1340:1342	Generally, ABS and EBS could be used as food thickener or frozen food additives.
31549696	0	0	theme	ion	104:106	arg1	delivery					108:115	dual and decoupled therapeutic ion delivery	73:115	dual and decoupled therapeutic ion delivery	73:115	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	1	1	theme	growth	348:353	arg1	factors					355:361	expensive and potentially problematic growth factors	310:361	expensive and potentially problematic growth factors	310:361	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	7	2	from	combination	1245:1255	arg1	conjunction					1291:1301	conjunction	1291:1301	conjunction with copper and strontium	1291:1327	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	5	3	theme	morphology	947:956	arg1	terms					938:942	terms	938:942	terms	938:942	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	2	4	theme	therapeutic	506:516	arg1	ions					518:521	therapeutic ions	506:521	therapeutic ions	506:521	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	1	5	theme	factors	355:361	arg1	use					303:305	the use	299:305	the use of expensive and potentially problematic growth factors and other sensitive biomolecules	299:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	8	6	theme	composite	1561:1569	arg1	components					1543:1552	both components	1538:1552	both components of the composite, leading to tailored biological effects	1538:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	6	7	theme	preliminary	1129:1139	arg1	characterization					1152:1167	a preliminary biological characterization	1127:1167	a preliminary biological characterization using cell line osteoblasts	1127:1195	In addition, a preliminary biological characterization using cell line osteoblasts was performed.
31549696	1	8	theme	bone	265:268	arg1	engineering					277:287	bone tissue engineering	265:287	bone tissue engineering	265:287	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	7	9	contain	has	1329:1331	arg2	potential					1339:1347	great potential	1333:1347	great potential	1333:1347	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	7	9	contain	has	1329:1331	arg1	combination					1245:1255	the combination	1241:1255	the combination of chitosan and hydroxyapatite in conjunction with copper and strontium	1241:1327	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	5	10	theme	calcium	1061:1067	arg1	phosphate					1069:1077	novel calcium phosphate	1055:1077	novel calcium phosphate	1055:1077	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	4	11	theme	freeze-dried	866:877	arg1	scaffolds					889:897	freeze-dried composite scaffolds	866:897	freeze-dried composite scaffolds	866:897	We combined copper(ii)-chitosan and strontium-hydroxyapatite into freeze-dried composite scaffolds.
31549696	8	12	theme	ideal	1416:1420	arg1	technology					1422:1431	an ideal technology	1413:1431	an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis	1413:1672	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	8	12	theme	ideal	1416:1420	arg1	composites					1395:1404	Chitosan/HA composites	1383:1404	Chitosan/HA composites	1383:1404	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	1	13	theme	tissue	270:275	arg1	engineering					277:287	bone tissue engineering	265:287	bone tissue engineering	265:287	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	5	14	with	contact	1082:1088	arg1	fluids					1106:1111	biological fluids	1095:1111	biological fluids	1095:1111	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	1	15	theme	other	367:371	arg1	biomolecules					383:394	other sensitive biomolecules	367:394	other sensitive biomolecules	367:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	6	16	theme	biological	1141:1150	arg1	characterization					1152:1167	a preliminary biological characterization	1127:1167	a preliminary biological characterization using cell line osteoblasts	1127:1195	In addition, a preliminary biological characterization using cell line osteoblasts was performed.
31549696	5	17	theme	bioactivity	985:995	arg1	terms					938:942	terms	938:942	terms	938:942	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	1	18	theme	sensitive	373:381	arg1	biomolecules					383:394	other sensitive biomolecules	367:394	other sensitive biomolecules	367:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	4	19	theme	copper	812:817	arg1	-chitosan					822:830	copper(ii)-chitosan	812:830	copper(ii)-chitosan	812:830	We combined copper(ii)-chitosan and strontium-hydroxyapatite into freeze-dried composite scaffolds.
31549696	8	20	from	ions	1530:1533	arg1	components					1543:1552	both components	1538:1552	both components of the composite, leading to tailored biological effects	1538:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	2	21	theme	copper	526:531	arg1	derivative					546:555	a copper(ii)-chitosan derivative	524:555	a copper(ii)-chitosan derivative	524:555	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	1	22	theme	biomolecules	383:394	arg1	use					303:305	the use	299:305	the use of expensive and potentially problematic growth factors and other sensitive biomolecules	299:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	2	23	dep	preparation	436:446	arg1	hydroxyapatite					585:598	a strontium-substituted hydroxyapatite	561:598	a strontium-substituted hydroxyapatite	561:598	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	2	23	dep	preparation	436:446	arg1	the					421:423	the	421:423	the	421:423	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	2	23	dep	preparation	436:446	arg1	derivative					546:555	a copper(ii)-chitosan derivative	524:555	a copper(ii)-chitosan derivative	524:555	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	8	24	theme	scaffolds	1475:1483	arg1	development					1441:1451	the development	1437:1451	the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis	1437:1672	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	1	25	theme	metal	179:183	arg1	ions					185:188	metal ions	179:188	metal ions	179:188	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	0	26	theme	composite	24:32	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	26	theme	composite	24:32	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	26	theme	composite	24:32	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	3	27	theme	strontium	746:754	arg1	release					735:741	a more sustained release	718:741	a more sustained release of strontium in order to achieve long-term osteogenesis	718:797	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	8	28	from	arrays	1508:1513	arg1	components					1543:1552	both components	1538:1552	both components of the composite, leading to tailored biological effects	1538:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	5	29	from	deposition	1041:1050	arg1	contact					1082:1088	contact	1082:1088	contact with biological fluids	1082:1111	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	1	30	theme	ions	185:188	arg1	ions					158:161	Therapeutic metal ions	140:161	Therapeutic metal ions	140:161	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	1	30	theme	ions	185:188	arg1	family					169:174	a family	167:174	a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules	167:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	0	31	theme	Chitosan/hydroxyapatite	0:22	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	31	theme	Chitosan/hydroxyapatite	0:22	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	31	theme	Chitosan/hydroxyapatite	0:22	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	3	32	theme	long-term	776:784	arg1	osteogenesis					786:797	long-term osteogenesis	776:797	long-term osteogenesis	776:797	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	8	33	from	components	1543:1552	arg1	arrays					1508:1513	a complex arrays	1498:1513	a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects	1498:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	8	34	theme	a	1498:1498	arg1	arrays					1508:1513	a complex arrays	1498:1513	a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects	1498:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	8	35	theme	ions	1530:1533	arg1	arrays					1508:1513	a complex arrays	1498:1513	a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects	1498:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	0	36	theme	tissue	39:44	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	36	theme	tissue	39:44	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	36	theme	tissue	39:44	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	8	37	theme	antibacterial	1617:1629	arg1	activity					1631:1638	antibacterial activity	1617:1638	antibacterial activity	1617:1638	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	2	38	contain	containing	495:504	arg1	platforms					485:493	two material platforms	472:493	two material platforms containing therapeutic ions	472:521	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	2	38	contain	containing	495:504	arg2	ions					518:521	therapeutic ions	506:521	therapeutic ions	506:521	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	8	39	theme	tissue	1456:1461	arg1	scaffolds					1475:1483	tissue engineering scaffolds	1456:1483	tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis	1456:1672	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	3	40	theme	ideal	627:631	arg1	profiles					645:652	ideal ion release profiles	627:652	ideal ion release profiles	627:652	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	2	41	theme	successful	425:434	arg1	preparation					436:446	preparation	436:446	preparation	436:446	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	0	42	theme	bone	34:37	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	42	theme	bone	34:37	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	42	theme	bone	34:37	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	8	43	theme	biological	1592:1601	arg1	effects					1603:1609	tailored biological effects	1583:1609	tailored biological effects	1583:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	8	44	theme	engineering	1463:1473	arg1	scaffolds					1475:1483	tissue engineering scaffolds	1456:1483	tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis	1456:1672	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	2	45	theme	platforms	485:493	arg1	characterization					452:467	characterization	452:467	characterization	452:467	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	2	45	theme	platforms	485:493	arg1	preparation					436:446	preparation	436:446	preparation	436:446	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	1	46	theme	specific	207:214	arg1	properties					227:236	specific biological properties	207:236	specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules	207:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	7	47	theme	novel	1366:1370	arg1	scaffolds					1372:1380	novel scaffolds	1366:1380	novel scaffolds	1366:1380	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	8	48	theme	therapeutic	1518:1528	arg1	ions					1530:1533	therapeutic ions	1518:1533	therapeutic ions in both components of the composite, leading to tailored biological effects	1518:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	0	49	dep	dual	73:76	arg1	therapeutic					92:102	therapeutic	92:102	therapeutic	92:102	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	1	50	theme	biological	216:225	arg1	properties					227:236	specific biological properties	207:236	specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules	207:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	0	51	theme	engineering	46:56	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	51	theme	engineering	46:56	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	51	theme	engineering	46:56	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	2	52	theme	-chitosan	536:544	arg1	derivative					546:555	a copper(ii)-chitosan derivative	524:555	a copper(ii)-chitosan derivative	524:555	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	3	53	theme	burst	664:668	arg1	release					670:676	burst release	664:676	burst release of an antibacterial agent	664:702	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	3	54	theme	ion	633:635	arg1	profiles					645:652	ideal ion release profiles	627:652	ideal ion release profiles	627:652	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	8	55	theme	Chitosan/HA	1383:1393	arg1	technology					1422:1431	an ideal technology	1413:1431	an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis	1413:1672	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	8	55	theme	Chitosan/HA	1383:1393	arg1	composites					1395:1404	Chitosan/HA composites	1383:1404	Chitosan/HA composites	1383:1404	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	7	56	theme	chitosan	1260:1267	arg1	combination					1245:1255	the combination	1241:1255	the combination of chitosan and hydroxyapatite in conjunction with copper and strontium	1241:1327	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	0	57	theme	dual	73:76	arg1	delivery					108:115	dual and decoupled therapeutic ion delivery	73:115	dual and decoupled therapeutic ion delivery	73:115	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	1	58	theme	expensive	310:318	arg1	factors					355:361	expensive and potentially problematic growth factors	310:361	expensive and potentially problematic growth factors	310:361	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	0	59	dep	scaffolds	58:66	arg1	scaffolds					58:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds	0:66	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.	0:138	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	59	dep	scaffolds	58:66	arg1	strontium					129:137	strontium	129:137	strontium	129:137	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	0	59	dep	scaffolds	58:66	arg1	copper					118:123	copper	118:123	copper	118:123	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	5	60	theme	mechanical	959:968	arg1	properties					970:979	mechanical properties	959:979	mechanical properties	959:979	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	3	61	theme	sustained	725:733	arg1	release					735:741	a more sustained release	718:741	a more sustained release of strontium in order to achieve long-term osteogenesis	718:797	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	4	62	theme	composite	879:887	arg1	scaffolds					889:897	freeze-dried composite scaffolds	866:897	freeze-dried composite scaffolds	866:897	We combined copper(ii)-chitosan and strontium-hydroxyapatite into freeze-dried composite scaffolds.
31549696	5	63	theme	properties	970:979	arg1	terms					938:942	terms	938:942	terms	938:942	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	5	64	theme	biological	1095:1104	arg1	fluids					1106:1111	biological fluids	1095:1111	biological fluids	1095:1111	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	8	65	theme	complex	1500:1506	arg1	arrays					1508:1513	a complex arrays	1498:1513	a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects	1498:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	5	66	theme	phosphate	1069:1077	arg1	deposition					1041:1050	the deposition	1037:1050	the deposition of novel calcium phosphate in contact with biological fluids	1037:1111	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	1	67	theme	Therapeutic	140:150	arg1	ions					158:161	Therapeutic metal ions	140:161	Therapeutic metal ions	140:161	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	1	67	theme	Therapeutic	140:150	arg1	family					169:174	a family	167:174	a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules	167:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	6	68	theme	cell	1175:1178	arg1	osteoblasts					1185:1195	cell line osteoblasts	1175:1195	cell line osteoblasts	1175:1195	In addition, a preliminary biological characterization using cell line osteoblasts was performed.
31549696	2	69	theme	strontium-substituted	563:583	arg1	hydroxyapatite					585:598	a strontium-substituted hydroxyapatite	561:598	a strontium-substituted hydroxyapatite	561:598	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	3	70	theme	antibacterial	684:696	arg1	agent					698:702	an antibacterial agent	681:702	an antibacterial agent	681:702	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	7	71	with	conjunction	1291:1301	arg1	strontium					1319:1327	strontium	1319:1327	strontium	1319:1327	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	7	71	with	conjunction	1291:1301	arg1	copper					1308:1313	copper	1308:1313	copper	1308:1313	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	1	72	theme	metal	152:156	arg1	ions					158:161	Therapeutic metal ions	140:161	Therapeutic metal ions	140:161	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	1	72	theme	metal	152:156	arg1	family					169:174	a family	167:174	a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules	167:394	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	6	73	theme	line	1180:1183	arg1	osteoblasts					1185:1195	cell line osteoblasts	1175:1195	cell line osteoblasts	1175:1195	In addition, a preliminary biological characterization using cell line osteoblasts was performed.
31549696	0	74	theme	decoupled	82:90	arg1	delivery					108:115	dual and decoupled therapeutic ion delivery	73:115	dual and decoupled therapeutic ion delivery	73:115	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	3	75	theme	agent	698:702	arg1	release					670:676	burst release	664:676	burst release of an antibacterial agent	664:702	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	3	76	theme	release	637:643	arg1	profiles					645:652	ideal ion release profiles	627:652	ideal ion release profiles	627:652	These biomaterials showed ideal ion release profiles, offering burst release of an antibacterial agent together with a more sustained release of strontium in order to achieve long-term osteogenesis.
31549696	7	77	theme	scaffolds	1372:1380	arg1	design					1356:1361	the design	1352:1361	the design of novel scaffolds	1352:1380	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	2	78	theme	material	476:483	arg1	platforms					485:493	two material platforms	472:493	two material platforms containing therapeutic ions	472:521	In this work, we report the successful preparation and characterization of two material platforms containing therapeutic ions: a copper(ii)-chitosan derivative and a strontium-substituted hydroxyapatite.
31549696	1	79	theme	problematic	336:346	arg1	factors					355:361	expensive and potentially problematic growth factors	310:361	expensive and potentially problematic growth factors	310:361	Therapeutic metal ions are a family of metal ions characterized by specific biological properties that could be exploited in bone tissue engineering, avoiding the use of expensive and potentially problematic growth factors and other sensitive biomolecules.
31549696	5	80	theme	novel	1055:1059	arg1	phosphate					1069:1077	novel calcium phosphate	1055:1077	novel calcium phosphate	1055:1077	These scaffolds were characterized in terms of morphology, mechanical properties and bioactivity, defined here as the ability to trigger the deposition of novel calcium phosphate in contact with biological fluids.
31549696	0	81	with	scaffolds	58:66	arg1	delivery					108:115	dual and decoupled therapeutic ion delivery	73:115	dual and decoupled therapeutic ion delivery	73:115	Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium.
31549696	7	82	theme	great	1333:1337	arg1	potential					1339:1347	great potential	1333:1347	great potential	1333:1347	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
31549696	8	83	theme	tailored	1583:1590	arg1	effects					1603:1609	tailored biological effects	1583:1609	tailored biological effects	1583:1609	Chitosan/HA composites can be an ideal technology for the development of tissue engineering scaffolds that deliver a complex arrays of therapeutic ions in both components of the composite, leading to tailored biological effects, from antibacterial activity, to osteogenesis and angiogenesis.
31549696	7	84	theme	hydroxyapatite	1273:1286	arg1	combination					1245:1255	the combination	1241:1255	the combination of chitosan and hydroxyapatite in conjunction with copper and strontium	1241:1327	Our results highlighted that the combination of chitosan and hydroxyapatite in conjunction with copper and strontium has great potential in the design of novel scaffolds.
30599989	1	0	theme	cellulose/chitosan-based	327:350	arg1	films					352:356	cellulose/chitosan-based films	327:356	cellulose/chitosan-based films	327:356	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	1	theme	films	352:356	arg1	physico-chemical					231:246	physico-chemical	231:246	physico-chemical	231:246	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	5	2	theme	nonrenewable	1008:1019	arg1	generation					1029:1038	toxic and nonrenewable e-waste generation	998:1038	toxic and nonrenewable e-waste generation	998:1038	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	1	3	theme	bio-based	150:158	arg1	films					165:169	bio-based thin films	150:169	bio-based thin films	150:169	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	4	4	theme	storage	847:853	arg1	capacity					855:862	the charge storage capacity	836:862	the charge storage capacity	836:862	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	0	5	theme	green	89:93	arg1	electronics					95:105	green electronics	89:105	green electronics	89:105	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	4	6	theme	charge	840:845	arg1	capacity					855:862	the charge storage capacity	836:862	the charge storage capacity	836:862	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	5	7	theme	thin	879:882	arg1	films					884:888	The bio-based thin films	865:888	The bio-based thin films	865:888	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	1	8	theme	thin	160:163	arg1	films					165:169	bio-based thin films	150:169	bio-based thin films	150:169	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	5	9	theme	bio-based	869:877	arg1	films					884:888	The bio-based thin films	865:888	The bio-based thin films	865:888	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	2	10	theme	constituents	446:457	arg1	dispersion					428:437	a suitable dispersion	417:437	a suitable dispersion of the constituents observed by SEM and FTIR	417:482	The films were prepared by solution casting and presented a suitable dispersion of the constituents observed by SEM and FTIR.
30599989	0	11	theme	thin	10:13	arg1	films					15:19	Bio-based thin films	0:19	Bio-based thin films of cellulose nanofibrils	0:44	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	4	12	theme	capacitance	721:731	arg1	measurements					705:716	measurements	705:716	measurements of capacitance	705:731	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	2	13	theme	solution	386:393	arg1	casting					395:401	solution casting	386:401	solution casting	386:401	The films were prepared by solution casting and presented a suitable dispersion of the constituents observed by SEM and FTIR.
30599989	0	14	theme	Bio-based	0:8	arg1	films					15:19	Bio-based thin films	0:19	Bio-based thin films of cellulose nanofibrils	0:44	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	5	15	theme	e-waste	1021:1027	arg1	generation					1029:1038	toxic and nonrenewable e-waste generation	998:1038	toxic and nonrenewable e-waste generation	998:1038	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	4	16	theme	glycerol	790:797	arg1	additions					763:771	the additions	759:771	the additions of magnetite and glycerol more than doubling the εr	759:823	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	3	17	theme	higher	540:545	arg1	flexibility					547:557	a higher flexibility	538:557	a higher flexibility	538:557	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	5	18	theme	electronics	971:981	arg1	production					951:960	the production	947:960	the production of green electronics	947:981	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	1	19	theme	barrier	278:284	arg1	properties					286:295	barrier properties	278:295	barrier properties	278:295	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	4	20	theme	composite	676:684	arg1	films					686:690	the composite films	672:690	the composite films	672:690	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	2	21	theme	suitable	419:426	arg1	dispersion					428:437	a suitable dispersion	417:437	a suitable dispersion of the constituents observed by SEM and FTIR	417:482	The films were prepared by solution casting and presented a suitable dispersion of the constituents observed by SEM and FTIR.
30599989	0	22	theme	nanofibrils	34:44	arg1	films					15:19	Bio-based thin films	0:19	Bio-based thin films of cellulose nanofibrils	0:44	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	0	23	from	application	74:84	arg1	electronics					95:105	green electronics	89:105	green electronics	89:105	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	3	24	theme	barrier	582:588	arg1	properties					590:599	lower barrier properties	576:599	lower barrier properties	576:599	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	4	25	theme	dielectric	643:652	arg1	εr					664:665	εr	664:665	εr	664:665	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	4	25	theme	dielectric	643:652	arg1	constant					654:661	The calculated dielectric constant	628:661	The calculated dielectric constant (εr) for the composite films	628:690	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	0	26	theme	cellulose	24:32	arg1	nanofibrils					34:44	cellulose nanofibrils	24:44	cellulose nanofibrils	24:44	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	4	27	theme	magnetite	776:784	arg1	additions					763:771	the additions	759:771	the additions of magnetite and glycerol more than doubling the εr	759:823	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	1	28	theme	magnetite	201:209	arg1	additions					188:196	the additions	184:196	the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films	184:356	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	5	29	contain	have	890:893	arg2	potential					895:903	potential	895:903	potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation	895:1038	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	5	29	contain	have	890:893	arg1	films					884:888	The bio-based thin films	865:888	The bio-based thin films	865:888	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	3	30	dep	150 °C	523:528	arg1	up					517:518	up	517:518	up	517:518	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	1	31	theme	dielectric	302:311	arg1	properties					313:322	the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties	227:322	properties	313:322	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	5	32	theme	toxic	998:1002	arg1	generation					1029:1038	toxic and nonrenewable e-waste generation	998:1038	toxic and nonrenewable e-waste generation	998:1038	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	3	33	theme	lower	576:580	arg1	properties					590:599	lower barrier properties	576:599	lower barrier properties	576:599	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	3	34	contain	had	534:536	arg1	films					489:493	The films	485:493	The films	485:493	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	3	34	contain	had	534:536	arg2	wettability					560:570	wettability	560:570	wettability	560:570	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	3	34	contain	had	534:536	arg2	flexibility					547:557	a higher flexibility	538:557	a higher flexibility	538:557	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	3	34	contain	had	534:536	arg2	properties					590:599	lower barrier properties	576:599	lower barrier properties	576:599	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	3	34	contain	had	534:536	arg1	stable					510:515	stable	510:515	stable	510:515	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	1	35	from	additions	188:196	arg1	physico-chemical					231:246	physico-chemical	231:246	physico-chemical	231:246	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	35	from	additions	188:196	arg1	properties					313:322	the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties	227:322	properties	313:322	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	36	dep	physico-chemical	231:246	arg1	wettability					262:272	wettability	262:272	wettability	262:272	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	36	dep	physico-chemical	231:246	arg1	properties					286:295	barrier properties	278:295	barrier properties	278:295	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	36	dep	physico-chemical	231:246	arg1	flexibility					249:259	flexibility	249:259	flexibility	249:259	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	3	37	theme	glycerol	618:625	arg1	addition					606:613	addition	606:613	addition of glycerol	606:625	The films were thermally stable up to 150 °C and had a higher flexibility, wettability and lower barrier properties upon addition of glycerol.
30599989	1	38	theme	work	130:133	arg1	objective					112:120	The objective	108:120	The objective of this work	108:133	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	1	39	theme	glycerol	215:222	arg1	additions					188:196	the additions	184:196	the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films	184:356	The objective of this work was to prepare bio-based thin films and evaluate the additions of magnetite and glycerol on the physico-chemical (flexibility, wettability and barrier properties) and dielectric properties of cellulose/chitosan-based films.
30599989	0	40	theme	potential	64:72	arg1	application					74:84	potential application	64:84	potential application in green electronics	64:105	Bio-based thin films of cellulose nanofibrils and magnetite for potential application in green electronics.
30599989	4	41	theme	calculated	632:641	arg1	εr					664:665	εr	664:665	εr	664:665	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	4	41	theme	calculated	632:641	arg1	constant					654:661	The calculated dielectric constant	628:661	The calculated dielectric constant (εr) for the composite films	628:690	The calculated dielectric constant (εr) for the composite films was based on measurements of capacitance, at 100 and 1000 Hz, with the additions of magnetite and glycerol more than doubling the εr increasing the charge storage capacity.
30599989	5	42	theme	green	965:969	arg1	electronics					971:981	green electronics	965:981	green electronics	965:981	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30599989	5	43	from	capacitors	933:942	arg1	production					951:960	the production	947:960	the production of green electronics	947:981	The bio-based thin films have potential to be used as insulators in capacitors on the production of green electronics thus, reducing toxic and nonrenewable e-waste generation.
30889667	4	0	theme	biological	1083:1092	arg1	evaluation					1094:1103	biological evaluation	1083:1103	biological evaluation	1083:1103	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	1	theme	several	1232:1238	arg1	probes					1263:1268	several independent analytical probes	1232:1268	several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies	1232:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	2	theme	soluble	627:633	arg1	network					649:655	the soluble atelocollagen network	623:655	the soluble atelocollagen network	623:655	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	3	theme	c-plane	672:678	arg1	growth					692:697	a peculiar c-plane orientation growth	661:697	a peculiar c-plane orientation growth	661:697	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	4	4	from	formation	1174:1182	arg1	it					1198:1199	it	1198:1199	it	1198:1199	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	1	5	theme	freeze-dry	189:198	arg1	gel					242:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	2	6	theme	osteointegrative	579:594	arg1	performance					596:606	a superior osteointegrative performance	568:606	a superior osteointegrative performance	568:606	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	1	7	theme	"	226:226	arg1	gel					242:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	4	8	theme	gel	1153:1155	arg1	scaffold					1157:1164	the FD-NanoCliP gel scaffold	1137:1164	the FD-NanoCliP gel scaffold	1137:1164	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	6	9	theme	polysaccharide	1660:1673	arg1	network					1675:1681	the polysaccharide network	1656:1681	the polysaccharide network	1656:1681	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	1	10	theme	FD-NanoCliP	229:239	arg1	gel					242:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	6	11	located	found	1647:1651	arg1	network					1675:1681	the polysaccharide network	1656:1681	the polysaccharide network	1656:1681	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	6	11	located	found	1647:1651	arg2	bioactivity					1635:1645	the superior bioactivity	1622:1645	the superior bioactivity found in the polysaccharide network	1622:1681	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	3	12	theme	gel	824:826	arg1	scaffold					828:835	the FD-NanoCliP gel scaffold	808:835	the FD-NanoCliP gel scaffold	808:835	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	4	13	from	chemistry	1124:1132	arg1	it					1198:1199	it	1198:1199	it	1198:1199	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	14	theme	crystallites	710:721	arg1	network					649:655	the soluble atelocollagen network	623:655	the soluble atelocollagen network	623:655	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	14	theme	crystallites	710:721	arg1	growth					692:697	a peculiar c-plane orientation growth	661:697	a peculiar c-plane orientation growth	661:697	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	1	15	theme	natural	140:146	arg1	scaffold					163:170	A natural polysaccharide scaffold	138:170	A natural polysaccharide scaffold	138:170	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	4	16	theme	apatite	1187:1193	arg1	formation					1174:1182	the formation	1170:1182	the formation of apatite on it	1170:1199	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	16	theme	apatite	1187:1193	arg1	chemistry					1124:1132	the peculiar chemistry	1111:1132	the peculiar chemistry of the FD-NanoCliP gel scaffold	1111:1164	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	3	17	theme	additional	841:850	arg1	purpose					852:858	an additional purpose	838:858	an additional purpose of this study	838:872	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	0	18	theme	polysaccharide	63:76	arg1	nanogel					78:84	natural polysaccharide nanogel	55:84	natural polysaccharide nanogel	55:84	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	2	19	theme	enamel	765:770	arg1	structure					744:752	the structure	740:752	the structure of natural enamel	740:770	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	1	20	theme	mesenchymal	349:359	arg1	line					377:380	the mouse mesenchymal progenitor cell line	339:380	the mouse mesenchymal progenitor cell line KUSA-A1	339:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	0	21	dep	study	131:135	arg1	response					11:18	Osteogenic response	0:18	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds	0:112	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	3	22	theme	study	868:872	arg1	purpose					852:858	an additional purpose	838:858	an additional purpose of this study	838:872	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	1	23	theme	cell	372:375	arg1	line					377:380	the mouse mesenchymal progenitor cell line	339:380	the mouse mesenchymal progenitor cell line KUSA-A1	339:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	2	24	theme	porous	547:552	arg1	gel					554:556	a physically crosslinked porous gel	522:556	a physically crosslinked porous gel	522:556	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	5	25	theme	polysaccharide	1515:1528	arg1	surface					1539:1545	the polysaccharide scaffold surface	1511:1545	the polysaccharide scaffold surface	1511:1545	This body of information consistently provided evidence for a peculiar chemistry developed in osteogenesis at the polysaccharide scaffold surface.
30889667	2	26	theme	orientation	680:690	arg1	growth					692:697	a peculiar c-plane orientation growth	661:697	a peculiar c-plane orientation growth	661:697	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	27	link	crosslinked	477:487	arg1	nanogels					489:496	chemically crosslinked nanogels	466:496	chemically crosslinked nanogels as building blocks	466:515	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	28	theme	amphiphilic	395:405	arg1	network					422:428	The amphiphilic polysaccharide network	391:428	The amphiphilic polysaccharide network	391:428	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	0	29	theme	Osteogenic	0:9	arg1	response					11:18	Osteogenic response	0:18	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds	0:112	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	6	30	from	available	1570:1578	arg1	atelocollagen					1591:1603	soluble atelocollagen	1583:1603	soluble atelocollagen	1583:1603	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	4	31	theme	analytical	1252:1261	arg1	probes					1263:1268	several independent analytical probes	1232:1268	several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies	1232:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	32	link	crosslinked	535:545	arg1	gel					554:556	a physically crosslinked porous gel	522:556	a physically crosslinked porous gel	522:556	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	33	theme	building	501:508	arg1	blocks					510:515	building blocks	501:515	building blocks	501:515	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	0	34	theme	progenitor	35:44	arg1	cells					46:50	mesenchymal progenitor cells	23:50	mesenchymal progenitor cells	23:50	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	3	35	theme	mesenchymal	998:1008	arg1	cells					1010:1014	mesenchymal cells	998:1014	mesenchymal cells	998:1014	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	4	36	theme	peculiar	1115:1122	arg1	chemistry					1124:1132	the peculiar chemistry	1111:1132	the peculiar chemistry of the FD-NanoCliP gel scaffold	1111:1164	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	1	37	dep	line	377:380	arg1	KUSA-A1					382:388	KUSA-A1	382:388	the mouse mesenchymal progenitor cell line KUSA-A1	339:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	1	38	with	comparison	261:270	arg1	scaffold					294:301	an atelocollagen scaffold	277:301	an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1	277:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	6	39	theme	Such	1548:1551	arg1	chemistry					1553:1561	Such chemistry	1548:1561	Such chemistry	1548:1561	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	4	40	theme	evaluation	1094:1103	arg1	kits					1105:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	41	theme	X-ray	1366:1370	arg1	spectroscopies					1385:1398	X-ray fluorescence spectroscopies	1366:1398	X-ray fluorescence spectroscopies	1366:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	5	42	theme	information	1414:1424	arg1	body					1406:1409	This body	1401:1409	This body of information	1401:1424	This body of information consistently provided evidence for a peculiar chemistry developed in osteogenesis at the polysaccharide scaffold surface.
30889667	2	43	theme	atelocollagen	635:647	arg1	network					649:655	the soluble atelocollagen network	623:655	the soluble atelocollagen network	623:655	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	4	44	theme	dispersive	1344:1353	arg1	X-ray					1355:1359	energy dispersive X-ray	1337:1359	energy dispersive X-ray	1337:1359	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	45	theme	crosslinked	477:487	arg1	nanogels					489:496	chemically crosslinked nanogels	466:496	chemically crosslinked nanogels as building blocks	466:515	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	2	46	theme	peculiar	663:670	arg1	growth					692:697	a peculiar c-plane orientation growth	661:697	a peculiar c-plane orientation growth	661:697	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	5	47	theme	peculiar	1463:1470	arg1	chemistry					1472:1480	a peculiar chemistry	1461:1480	a peculiar chemistry developed in osteogenesis at the polysaccharide scaffold surface	1461:1545	This body of information consistently provided evidence for a peculiar chemistry developed in osteogenesis at the polysaccharide scaffold surface.
30889667	4	48	theme	FD-NanoCliP	1141:1151	arg1	scaffold					1157:1164	the FD-NanoCliP gel scaffold	1137:1164	the FD-NanoCliP gel scaffold	1137:1164	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	3	49	theme	FD-NanoCliP	812:822	arg1	scaffold					828:835	the FD-NanoCliP gel scaffold	808:835	the FD-NanoCliP gel scaffold	808:835	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	6	50	theme	soluble	1583:1589	arg1	atelocollagen					1591:1603	soluble atelocollagen	1583:1603	soluble atelocollagen	1583:1603	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	1	51	theme	nanogel-crosslinked-porous	200:225	arg1	gel					242:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	"freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel	188:244	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	2	52	theme	superior	570:577	arg1	performance					596:606	a superior osteointegrative performance	568:606	a superior osteointegrative performance	568:606	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	4	53	theme	scaffold	1157:1164	arg1	formation					1174:1182	the formation	1170:1182	the formation of apatite on it	1170:1199	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	53	theme	scaffold	1157:1164	arg1	chemistry					1124:1132	the peculiar chemistry	1111:1132	the peculiar chemistry of the FD-NanoCliP gel scaffold	1111:1164	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	54	theme	natural	757:763	arg1	enamel					765:770	natural enamel	757:770	natural enamel	757:770	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	3	55	dep	assess	881:886	arg1	interpret					961:969	interpret	961:969	to interpret the osteogenic response of mesenchymal cells	958:1014	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	6	56	theme	superior	1626:1633	arg1	bioactivity					1635:1645	the superior bioactivity	1622:1645	the superior bioactivity found in the polysaccharide network	1622:1681	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	1	57	theme	polysaccharide	148:161	arg1	scaffold					163:170	A natural polysaccharide scaffold	138:170	A natural polysaccharide scaffold	138:170	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	3	58	theme	chemical	892:899	arg1	composition					901:911	its chemical composition	888:911	its chemical composition	888:911	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	0	59	theme	atelocollagen	90:102	arg1	scaffolds					104:112	atelocollagen scaffolds	90:112	atelocollagen scaffolds	90:112	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	1	60	theme	mouse	343:347	arg1	line					377:380	the mouse mesenchymal progenitor cell line	339:380	the mouse mesenchymal progenitor cell line KUSA-A1	339:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	6	61	from	bioactivity	1635:1645	arg1	key					1615:1617	key	1615:1617	key	1615:1617	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	2	62	theme	apatite	702:708	arg1	crystallites					710:721	apatite crystallites	702:721	apatite crystallites	702:721	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	1	63	theme	progenitor	361:370	arg1	line					377:380	the mouse mesenchymal progenitor cell line	339:380	the mouse mesenchymal progenitor cell line KUSA-A1	339:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	4	64	dep	kits	1105:1108	arg1	addition					1020:1027	addition	1020:1027	addition	1020:1027	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	65	from	scale	1287:1291	arg1	probes					1263:1268	several independent analytical probes	1232:1268	several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies	1232:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	0	66	theme	spectroscopic	117:129	arg1	study					131:135	A spectroscopic study	115:135	A spectroscopic study	115:135	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	6	67	from	key	1615:1617	arg1	bioactivity					1635:1645	the superior bioactivity	1622:1645	the superior bioactivity found in the polysaccharide network	1622:1681	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	2	68	theme	crosslinked	535:545	arg1	gel					554:556	a physically crosslinked porous gel	522:556	a physically crosslinked porous gel	522:556	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	5	69	theme	scaffold	1530:1537	arg1	surface					1539:1545	the polysaccharide scaffold surface	1511:1545	the polysaccharide scaffold surface	1511:1545	This body of information consistently provided evidence for a peculiar chemistry developed in osteogenesis at the polysaccharide scaffold surface.
30889667	4	70	theme	independent	1240:1250	arg1	probes					1263:1268	several independent analytical probes	1232:1268	several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies	1232:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	2	71	theme	polysaccharide	407:420	arg1	network					422:428	The amphiphilic polysaccharide network	391:428	The amphiphilic polysaccharide network	391:428	The amphiphilic polysaccharide network, engineered in its structure to fit chemically crosslinked nanogels as building blocks into a physically crosslinked porous gel, revealed a superior osteointegrative performance as compared to the soluble atelocollagen network and a peculiar c-plane orientation growth of apatite crystallites, which resembled the structure of natural enamel.
30889667	4	72	theme	electron	1058:1065	arg1	microscopy					1068:1077	conventional (optical and electron) microscopy	1032:1077	microscopy	1068:1077	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	0	73	theme	mesenchymal	23:33	arg1	cells					46:50	mesenchymal progenitor cells	23:50	mesenchymal progenitor cells	23:50	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	1	74	theme	atelocollagen	280:292	arg1	scaffold					294:301	an atelocollagen scaffold	277:301	an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1	277:388	A natural polysaccharide scaffold, referred to as "freeze-dry nanogel-crosslinked-porous" (FD-NanoCliP) gel, was tested in comparison with an atelocollagen scaffold with respect to osteogenesis versus the mouse mesenchymal progenitor cell line KUSA-A1.
30889667	4	75	theme	molecular	1277:1285	arg1	scale					1287:1291	the molecular scale	1273:1291	the molecular scale	1273:1291	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	6	76	from	atelocollagen	1591:1603	arg1	available					1570:1578	available	1570:1578	available	1570:1578	Such chemistry is not available in soluble atelocollagen and it is key in the superior bioactivity found in the polysaccharide network.
30889667	3	77	theme	osteogenic	975:984	arg1	response					986:993	the osteogenic response	971:993	the osteogenic response of mesenchymal cells	971:1014	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	4	78	theme	microscopy	1068:1077	arg1	kits					1105:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	0	79	theme	cells	46:50	arg1	response					11:18	Osteogenic response	0:18	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds	0:112	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	4	80	theme	conventional	1032:1043	arg1	optical					1046:1052	conventional (optical and electron) microscopy	1032:1077	optical	1046:1052	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	81	theme	energy	1337:1342	arg1	X-ray					1355:1359	energy dispersive X-ray	1337:1359	energy dispersive X-ray	1337:1359	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	0	82	theme	natural	55:61	arg1	nanogel					78:84	natural polysaccharide nanogel	55:84	natural polysaccharide nanogel	55:84	Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study.
30889667	3	83	theme	cells	1010:1014	arg1	response					986:993	the osteogenic response	971:993	the osteogenic response of mesenchymal cells	971:1014	Besides evaluating osteogenesis in the FD-NanoCliP gel scaffold, an additional purpose of this study was to assess its chemical composition at the nanoscale and, through its knowledge, to interpret the osteogenic response of mesenchymal cells.
30889667	4	84	theme	optical	1046:1052	arg1	kits					1105:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	conventional (optical and electron) microscopy and biological evaluation kits	1032:1108	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
30889667	4	85	theme	fluorescence	1372:1383	arg1	spectroscopies					1385:1398	X-ray fluorescence spectroscopies	1366:1398	X-ray fluorescence spectroscopies	1366:1398	In addition to conventional (optical and electron) microscopy and biological evaluation kits, the peculiar chemistry of the FD-NanoCliP gel scaffold and the formation of apatite on it were characterized by means of several independent analytical probes at the molecular scale, which included Raman, cathodoluminescence, energy dispersive X-ray, and X-ray fluorescence spectroscopies.
29752122	8	0	theme	cell-laden	1201:1210	arg1	constructs					1212:1221	the cell-laden constructs	1197:1221	the cell-laden constructs	1197:1221	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	6	1	theme	C/MMT	952:956	arg1	scaffolds					958:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr2+-modified and non-modified C/MMT scaffolds.
29752122	7	2	theme	MTT	1035:1037	arg1	assay					1102:1106	MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay	1035:1106	MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay	1035:1106	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	11	3	theme	bone	1600:1603	arg1	engineering					1612:1622	bone tissue engineering	1600:1622	bone tissue engineering	1600:1622	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	2	4	with	scaffold	334:341	arg1	structure					373:381	an interconnected porous structure	348:381	an interconnected porous structure	348:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	5	5	theme	composite	806:814	arg1	scaffolds					816:824	composite scaffolds	806:824	composite scaffolds	806:824	The biocompatibility of composite scaffolds was evaluated in cell cultures.
29752122	5	6	theme	scaffolds	816:824	arg1	biocompatibility					786:801	The biocompatibility	782:801	The biocompatibility of composite scaffolds	782:824	The biocompatibility of composite scaffolds was evaluated in cell cultures.
29752122	3	7	dep	fourier	416:422	arg1	transform					424:432	transform	424:432	transform infrared spectroscopy	424:454	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	6	8	theme	Human	858:862	arg1	hOBs					877:880	hOBs	877:880	hOBs	877:880	Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr2+-modified and non-modified C/MMT scaffolds.
29752122	6	8	theme	Human	858:862	arg1	osteoblasts					864:874	Human osteoblasts	858:874	Human osteoblasts (hOBs)	858:881	Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr2+-modified and non-modified C/MMT scaffolds.
29752122	4	9	theme	non-modified	716:727	arg1	scaffolds					742:750	the non-modified and modified scaffolds	712:750	the non-modified and modified scaffolds	712:750	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	11	10	theme	promising	1574:1582	arg1	biomaterial					1584:1594	a promising biomaterial	1572:1594	a promising biomaterial for bone tissue engineering	1572:1622	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	11	10	theme	promising	1574:1582	arg1	composite					1555:1563	Sr2+-modified C/MMT composite	1535:1563	Sr2+-modified C/MMT composite	1535:1563	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	3	11	theme	X-ray	397:401	arg1	diffraction					403:413	X-ray diffraction	397:413	X-ray diffraction	397:413	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	12	dep	transform	424:432	arg1	infrared					434:441	infrared	434:441	transform infrared spectroscopy	424:454	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	11	13	theme	tissue	1605:1610	arg1	engineering					1612:1622	bone tissue engineering	1600:1622	bone tissue engineering	1600:1622	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	2	14	theme	C/MMT	318:322	arg1	scaffold					334:341	strontium (Sr2+) modified C/MMT composite scaffold	292:341	strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure	292:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	10	15	theme	present	1314:1320	arg1	Ions					1309:1312	Ions	1309:1312	Ions present in the MMT	1309:1331	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	10	16	theme	scaffold	1420:1427	arg1	system					1429:1434	the C/MMT scaffold system	1410:1434	the C/MMT scaffold system	1410:1434	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	6	17	theme	Sr2+-modified	921:933	arg1	scaffolds					958:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr2+-modified and non-modified C/MMT scaffolds.
29752122	10	18	theme	osteoblast	1387:1396	arg1	activity					1398:1405	osteoblast activity	1387:1405	osteoblast activity	1387:1405	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	9	19	theme	C/MMT	1253:1257	arg1	scaffolds					1259:1267	C/MMT scaffolds	1253:1267	C/MMT scaffolds	1253:1267	In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts.
29752122	2	20	theme	modified	309:316	arg1	scaffold					334:341	strontium (Sr2+) modified C/MMT composite scaffold	292:341	strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure	292:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	11	21	theme	scaffold	1503:1510	arg1	properties					1512:1521	scaffold properties	1503:1521	scaffold properties	1503:1521	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	2	22	theme	interconnected	351:364	arg1	structure					373:381	an interconnected porous structure	348:381	an interconnected porous structure	348:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	4	23	theme	study	665:669	arg1	aims					652:655	the aims	648:655	the aims of this study	648:669	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	9	24	contain	had	1269:1271	arg2	effects					1285:1291	no negative effects	1273:1291	no negative effects	1273:1291	In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts.
29752122	9	24	contain	had	1269:1271	arg1	scaffolds					1259:1267	C/MMT scaffolds	1253:1267	C/MMT scaffolds	1253:1267	In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts.
29752122	3	25	theme	thermal	457:463	arg1	analysis					477:484	thermal gravimetric analysis	457:484	thermal gravimetric analysis	457:484	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	7	26	theme	cell	978:981	arg1	viability					983:991	In-vitro cell viability	969:991	In-vitro cell viability	969:991	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	2	27	theme	Sr2+	303:306	arg1	scaffold					334:341	strontium (Sr2+) modified C/MMT composite scaffold	292:341	strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure	292:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	11	28	theme	MMT-chitosan	1481:1492	arg1	modification					1465:1476	Sr2+ modification	1460:1476	Sr2+ modification of MMT-chitosan	1460:1492	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	1	29	theme	improved	196:203	arg1	properties					205:214	improved properties	196:214	improved properties for bone tissue engineering applications	196:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	5	30	theme	cell	843:846	arg1	cultures					848:855	cell cultures	843:855	cell cultures	843:855	The biocompatibility of composite scaffolds was evaluated in cell cultures.
29752122	4	31	theme	culture	766:772	arg1	medium					774:779	the cell culture medium	757:779	the cell culture medium	757:779	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	4	32	theme	Sr2+	702:705	arg1	release					691:697	the release	687:697	the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium	687:779	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	6	33	theme	non-modified	939:950	arg1	scaffolds					958:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Sr2+-modified and non-modified C/MMT scaffolds	921:966	Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr2+-modified and non-modified C/MMT scaffolds.
29752122	9	34	theme	negative	1276:1283	arg1	effects					1285:1291	no negative effects	1273:1291	no negative effects	1273:1291	In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts.
29752122	1	35	theme	study	114:118	arg1	objective					96:104	The objective	92:104	The objective of this study	92:118	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	11	36	theme	Sr2+-modified	1535:1547	arg1	biomaterial					1584:1594	a promising biomaterial	1572:1594	a promising biomaterial for bone tissue engineering	1572:1622	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	11	36	theme	Sr2+-modified	1535:1547	arg1	composite					1555:1563	Sr2+-modified C/MMT composite	1535:1563	Sr2+-modified C/MMT composite	1535:1563	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	4	37	theme	cell	761:764	arg1	medium					774:779	the cell culture medium	757:779	the cell culture medium	757:779	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	8	38	used	used	1177:1180	arg2	staining					1149:1156	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	7	39	theme	In-vitro	969:976	arg1	viability					983:991	In-vitro cell viability	969:991	In-vitro cell viability	969:991	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	10	40	from	MMT	1329:1331	arg1	present					1314:1320	present	1314:1320	present	1314:1320	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	11	41	theme	C/MMT	1549:1553	arg1	biomaterial					1584:1594	a promising biomaterial	1572:1594	a promising biomaterial for bone tissue engineering	1572:1622	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	11	41	theme	C/MMT	1549:1553	arg1	composite					1555:1563	Sr2+-modified C/MMT composite	1535:1563	Sr2+-modified C/MMT composite	1535:1563	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	1	42	theme	bone	220:223	arg1	applications					244:255	bone tissue engineering applications	220:255	bone tissue engineering applications	220:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	3	43	theme	composite	621:629	arg1	scaffold					631:638	the composite scaffold	617:638	the composite scaffold	617:638	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	8	44	theme	Live/dead	1134:1142	arg1	staining					1149:1156	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	2	45	theme	composite	324:332	arg1	scaffold					334:341	strontium (Sr2+) modified C/MMT composite scaffold	292:341	strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure	292:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	10	46	theme	culture	1361:1367	arg1	medium					1369:1374	the cell culture medium	1352:1374	the cell culture medium	1352:1374	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	1	47	theme	tissue	225:230	arg1	applications					244:255	bone tissue engineering applications	220:255	bone tissue engineering applications	220:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	0	48	theme	tissue	63:68	arg1	scaffold					82:89	bone tissue engineering scaffold	58:89	bone tissue engineering scaffold	58:89	Strontium-modified chitosan/montmorillonite composites as bone tissue engineering scaffold.
29752122	4	49	theme	modified	733:740	arg1	scaffolds					742:750	the non-modified and modified scaffolds	712:750	the non-modified and modified scaffolds	712:750	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	7	50	theme	DNA	1112:1114	arg1	analysis					1124:1131	DNA content analysis	1112:1131	DNA content analysis	1112:1131	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	3	51	theme	electron	500:507	arg1	microscopy					509:518	scanning electron microscopy	491:518	scanning electron microscopy (SEM)	491:524	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	51	theme	electron	500:507	arg1	SEM					521:523	SEM	521:523	SEM	521:523	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	2	52	theme	freeze-drying	267:279	arg1	technique					281:289	the freeze-drying technique	263:289	the freeze-drying technique	263:289	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	10	53	theme	cell	1356:1359	arg1	medium					1369:1374	the cell culture medium	1352:1374	the cell culture medium	1352:1374	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	1	54	theme	engineering	232:242	arg1	applications					244:255	bone tissue engineering applications	220:255	bone tissue engineering applications	220:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	0	55	theme	bone	58:61	arg1	scaffold					82:89	bone tissue engineering scaffold	58:89	bone tissue engineering scaffold	58:89	Strontium-modified chitosan/montmorillonite composites as bone tissue engineering scaffold.
29752122	3	56	theme	gravimetric	465:475	arg1	analysis					477:484	thermal gravimetric analysis	457:484	thermal gravimetric analysis	457:484	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	10	57	attach	present	1314:1320	arg2	Ions					1309:1312	Ions	1309:1312	Ions present in the MMT	1309:1331	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	10	57	attach	present	1314:1320	arg1	MMT					1329:1331	the MMT	1325:1331	the MMT	1325:1331	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	11	58	theme	Sr2+	1460:1463	arg1	modification					1465:1476	Sr2+ modification	1460:1476	Sr2+ modification of MMT-chitosan	1460:1492	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	10	59	from	present	1314:1320	arg1	MMT					1329:1331	the MMT	1325:1331	the MMT	1325:1331	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	3	60	theme	scaffold	631:638	arg1	morphology					590:599	surface morphology	582:599	surface morphology	582:599	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	60	theme	scaffold	631:638	arg1	porosity					605:612	porosity	605:612	porosity	605:612	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	60	theme	scaffold	631:638	arg1	properties					570:579	the structural properties	555:579	the structural properties	555:579	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	7	61	theme	‑2,5‑diphenyltetrazolium	1068:1091	arg1	bromide					1093:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	7	61	theme	‑2,5‑diphenyltetrazolium	1068:1091	arg1	MTT					1035:1037	MTT	1035:1037	MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay	1035:1106	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	7	62	theme	content	1116:1122	arg1	analysis					1124:1131	DNA content analysis	1112:1131	DNA content analysis	1112:1131	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	0	63	theme	engineering	70:80	arg1	scaffold					82:89	bone tissue engineering scaffold	58:89	bone tissue engineering scaffold	58:89	Strontium-modified chitosan/montmorillonite composites as bone tissue engineering scaffold.
29752122	8	64	theme	cell	1144:1147	arg1	staining					1149:1156	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM	1134:1170	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	1	65	theme	chitosan/montmorillonite	134:157	arg1	scaffolds					177:185	chitosan/montmorillonite (C/MMT) composite scaffolds	134:185	chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications	134:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	7	66	theme	4,5‑dimethylthiasol‑2‑yl	1043:1066	arg1	bromide					1093:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	7	66	theme	4,5‑dimethylthiasol‑2‑yl	1043:1066	arg1	MTT					1035:1037	MTT	1035:1037	MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay	1035:1106	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	2	67	theme	porous	366:371	arg1	structure					373:381	an interconnected porous structure	348:381	an interconnected porous structure	348:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	4	68	theme	aims	652:655	arg1	One					641:643	One	641:643	One	641:643	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	4	68	theme	aims	652:655	arg1	aims					652:655	the aims	648:655	the aims of this study	648:669	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	3	69	theme	surface	582:588	arg1	morphology					590:599	surface morphology	582:599	surface morphology	582:599	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	2	70	theme	strontium	292:300	arg1	scaffold					334:341	strontium (Sr2+) modified C/MMT composite scaffold	292:341	strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure	292:381	With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced.
29752122	8	71	dep	staining	1149:1156	arg1	SEM					1168:1170	SEM	1168:1170	SEM	1168:1170	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	8	71	dep	staining	1149:1156	arg1	assay					1158:1162	assay	1158:1162	assay	1158:1162	Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs.
29752122	1	72	theme	C/MMT	160:164	arg1	scaffolds					177:185	chitosan/montmorillonite (C/MMT) composite scaffolds	134:185	chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications	134:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	4	73	from	scaffolds	742:750	arg1	release					691:697	the release	687:697	the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium	687:779	One of the aims of this study was to document the release of Sr2+ from the non-modified and modified scaffolds into the cell culture medium.
29752122	10	74	theme	C/MMT	1414:1418	arg1	system					1429:1434	the C/MMT scaffold system	1410:1434	the C/MMT scaffold system	1410:1434	Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system.
29752122	7	75	theme	3‑	1040:1041	arg1	bromide					1093:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide	1040:1099	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	7	75	theme	3‑	1040:1041	arg1	MTT					1035:1037	MTT	1035:1037	MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay	1035:1106	In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis.
29752122	3	76	theme	structural	559:568	arg1	properties					570:579	the structural properties	555:579	the structural properties	555:579	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	77	theme	scanning	491:498	arg1	microscopy					509:518	scanning electron microscopy	491:518	scanning electron microscopy (SEM)	491:524	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	3	77	theme	scanning	491:498	arg1	SEM					521:523	SEM	521:523	SEM	521:523	X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold.
29752122	9	78	theme	In-vitro	1224:1231	arg1	studies					1233:1239	In-vitro studies	1224:1239	In-vitro studies	1224:1239	In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts.
29752122	1	79	theme	composite	167:175	arg1	scaffolds					177:185	chitosan/montmorillonite (C/MMT) composite scaffolds	134:185	chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications	134:255	The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications.
29752122	11	80	mod	modification	1465:1476	arg1	MMT-chitosan					1481:1492	MMT-chitosan	1481:1492	MMT-chitosan	1481:1492	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
29752122	11	80	mod	modification	1465:1476	arg3	Sr2+					1460:1463	Sr2+ modification	1460:1476	Sr2+ modification of MMT-chitosan	1460:1492	Findings indicate that Sr2+ modification of MMT-chitosan improves scaffold properties, suggesting Sr2+-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
31747567	2	0	theme	buffer	456:461	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	0	theme	buffer	456:461	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	1	1	theme	simple	293:298	arg1	method					313:318	a simple freeze-dried method	291:318	a simple freeze-dried method	291:318	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	2	theme	SA	137:138	arg1	PMs					159:161	PMs	159:161	PMs	159:161	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	2	theme	SA	137:138	arg1	materials					148:156	silk fibroin (SF)/sodium alginate (SA) porous materials	102:156	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	3	theme	1-ethyl-3-	213:222	arg1	EDC					261:263	EDC	261:263	EDC	261:263	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	3	theme	1-ethyl-3-	213:222	arg1	carbodiimide					247:258	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	213:258	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	213:264	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	2	4	theme	Phosphate	446:454	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	4	theme	Phosphate	446:454	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	1	5	theme	freeze-dried	300:311	arg1	method					313:318	a simple freeze-dried method	291:318	a simple freeze-dried method	291:318	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	4	6	theme	50/50	779:783	arg1	PMs					785:787	SF/SA 50/50 PMs	773:787	SF/SA 50/50 PMs	773:787	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	2	7	used	used	504:507	arg2	control					514:520	a control	512:520	a control	512:520	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	7	used	used	504:507	arg2	experiment					333:342	Degradation experiment	321:342	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme	321:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	4	8	theme	enzyme	840:845	arg1	degradation					847:857	enzyme degradation	840:857	enzyme degradation	840:857	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	4	9	theme	SF/SA	773:777	arg1	PMs					785:787	SF/SA 50/50 PMs	773:787	SF/SA 50/50 PMs	773:787	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	2	10	theme	Degradation	321:331	arg1	control					514:520	a control	512:520	a control	512:520	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	10	theme	Degradation	321:331	arg1	experiment					333:342	Degradation experiment	321:342	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme	321:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	4	11	contain	possessed	795:803	arg1	PMs					785:787	SF/SA 50/50 PMs	773:787	SF/SA 50/50 PMs	773:787	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	4	11	contain	possessed	795:803	arg2	integrity					805:813	integrity	805:813	integrity of pore structure	805:831	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	6	12	theme	degradation	1378:1388	arg1	behavior					1390:1397	desired degradation behavior	1370:1397	desired degradation behavior	1370:1397	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	6	13	theme	specific	1404:1411	arg1	applications					1413:1424	specific applications	1404:1424	specific applications for the SF/SA composite PMs	1404:1452	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	4	14	theme	pure	754:757	arg1	PMs					762:764	the pure SF PMs	750:764	the pure SF PMs	750:764	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	4	15	theme	Collagenase	692:702	arg1	IA					704:705	Collagenase IA	692:705	Collagenase IA	692:705	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	1	16	theme	porous	141:146	arg1	PMs					159:161	PMs	159:161	PMs	159:161	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	16	theme	porous	141:146	arg1	materials					148:156	silk fibroin (SF)/sodium alginate (SA) porous materials	102:156	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	2	17	theme	PBS	471:473	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	17	theme	PBS	471:473	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	5	18	dep	in	1022:1023	arg1	vivo					1025:1028	vivo	1025:1028	vivo	1025:1028	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	2	19	dep	18 days	401:407	arg1	up					395:396	up	395:396	up	395:396	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	20	theme	Collagenase	412:422	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	20	theme	Collagenase	412:422	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	3	21	dep	showed	535:540	arg1	exhibited					558:566	exhibited	558:566	showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution	535:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	0	22	theme	fibroin/sodium	5:18	arg1	porous					39:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	2	23	from	37 °C	439:443	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	23	from	37 °C	439:443	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	4	24	theme	PMs	762:764	arg1	collapse					738:745	collapse	738:745	collapse of the pure SF PMs	738:764	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	5	25	from	structure	906:914	arg1	PMs					939:941	the SF/SA PMs	929:941	the SF/SA PMs	929:941	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	2	26	theme	PMs	353:355	arg1	control					514:520	a control	512:520	a control	512:520	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	26	theme	PMs	353:355	arg1	experiment					333:342	Degradation experiment	321:342	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme	321:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	0	27	theme	Silk	0:3	arg1	porous					39:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	5	28	theme	subcutaneous	1045:1056	arg1	implantation					1058:1069	subcutaneous implantation	1045:1069	subcutaneous implantation in rats	1045:1077	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	4	29	theme	SF	759:760	arg1	PMs					762:764	the pure SF PMs	750:764	the pure SF PMs	750:764	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	6	30	theme	desired	1370:1376	arg1	behavior					1390:1397	desired degradation behavior	1370:1397	desired degradation behavior	1370:1397	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	2	31	theme	SF/SA	347:351	arg1	PMs					353:355	SF/SA PMs	347:355	SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme	347:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	0	32	theme	composite	29:37	arg1	porous					39:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	4	33	theme	pore	818:821	arg1	structure					823:831	pore structure	818:831	pore structure	818:831	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	5	34	theme	SF/SA	933:937	arg1	PMs					939:941	the SF/SA PMs	929:941	the SF/SA PMs	929:941	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	1	35	theme	silk	102:105	arg1	SF					116:117	SF	116:117	SF	116:117	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	35	theme	silk	102:105	arg1	fibroin					107:113	silk fibroin	102:113	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	5	36	theme	SF	923:924	arg1	structure					906:914	The crystalline structure	890:914	The crystalline structure of the SF in the SF/SA PMs	890:941	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	0	37	theme	alginate	20:27	arg1	porous					39:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous	0:44	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	3	38	theme	Collagenase	646:656	arg1	solution					661:668	Collagenase IA solution	646:668	Collagenase IA solution	646:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	39	theme	weight	585:590	arg1	loss					592:595	weight loss	585:595	weight loss	585:595	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	1	40	theme	fibroin	107:113	arg1	PMs					159:161	PMs	159:161	PMs	159:161	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	40	theme	fibroin	107:113	arg1	materials					148:156	silk fibroin (SF)/sodium alginate (SA) porous materials	102:156	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	5	41	theme	host	1194:1197	arg1	animals					1199:1205	the host animals	1190:1205	the host animals	1190:1205	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	6	42	theme	composite	1291:1299	arg1	PMs					1301:1303	SF/SA composite PMs	1285:1303	SF/SA composite PMs	1285:1303	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	3	43	theme	IA	658:659	arg1	solution					661:668	Collagenase IA solution	646:668	Collagenase IA solution	646:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	44	theme	loss	592:595	arg1	weight					615:620	the weight	611:620	the weight retained within 18 d in Collagenase IA solution	611:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	44	theme	loss	592:595	arg1	rate					577:580	a lowest rate	568:580	a lowest rate of weight loss	568:595	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	44	theme	loss	592:595	arg1	%					606:606	about 68%	598:606	about 68% of the weight retained within 18 d in Collagenase IA solution	598:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	45	theme	SF/SA	542:546	arg1	PMs					554:556	SF/SA 50/50 PMs	542:556	SF/SA 50/50 PMs	542:556	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	1	46	theme	different	169:177	arg1	ratios					185:190	different blend ratios	169:190	different blend ratios	169:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	6	47	theme	SF/SA	1285:1289	arg1	PMs					1301:1303	SF/SA composite PMs	1285:1303	SF/SA composite PMs	1285:1303	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	5	48	theme	in	1022:1023	arg1	degradation					1030:1040	the in vivo degradation	1018:1040	the in vivo degradation by subcutaneous implantation in rats	1018:1077	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	4	49	theme	structure	823:831	arg1	integrity					805:813	integrity	805:813	integrity of pore structure	805:831	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	4	50	theme	SEM	671:673	arg1	images					675:680	SEM images	671:680	SEM images	671:680	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	3	51	theme	50/50	548:552	arg1	PMs					554:556	SF/SA 50/50 PMs	542:556	SF/SA 50/50 PMs	542:556	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	1	52	theme	blend	179:183	arg1	ratios					185:190	different blend ratios	169:190	different blend ratios	169:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	0	53	theme	controllable	61:72	arg1	degradation					74:84	controllable degradation	61:84	controllable degradation	61:84	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	1	54	theme	crosslinking	269:280	arg1	agent					282:286	crosslinking agent	269:286	crosslinking agent	269:286	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	2	55	theme	saline	463:468	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	55	theme	saline	463:468	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	5	56	theme	degradation	1030:1040	arg1	results					1007:1013	the results	1003:1013	the results of the in vivo degradation by subcutaneous implantation in rats	1003:1077	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	6	57	from	ratios	1263:1268	arg1	series					1275:1280	a series	1273:1280	a series of SF/SA composite PMs	1273:1303	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	4	58	theme	exposure	875:882	arg1	time					884:887	exposure time	875:887	exposure time	875:887	SEM images indicated Collagenase IA can degrade fibroin leading to collapse of the pure SF PMs, while SF/SA 50/50 PMs still possessed integrity of pore structure during enzyme degradation with increasing exposure time.
31747567	5	59	dep	d.	987:988	arg1	showed					1079:1084	showed	1079:1084	showed that all PMs can be degraded at different levels	1079:1133	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	5	60	theme	crystalline	894:904	arg1	structure					906:914	The crystalline structure	890:914	The crystalline structure of the SF in the SF/SA PMs	890:941	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	2	61	dep	PMs	353:355	arg1	investigated					382:393	investigated	382:393	have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme	357:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	6	62	theme	composite	1440:1448	arg1	PMs					1450:1452	the SF/SA composite PMs	1430:1452	the SF/SA composite PMs	1430:1452	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	5	63	theme	good	1150:1153	arg1	histocompatibility					1168:1185	good subcutaneous histocompatibility	1150:1185	good subcutaneous histocompatibility to the host animals	1150:1205	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	1	64	with	materials	148:156	arg1	ratios					185:190	different blend ratios	169:190	different blend ratios	169:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	3	65	theme	lowest	570:575	arg1	weight					615:620	the weight	611:620	the weight retained within 18 d in Collagenase IA solution	611:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	65	theme	lowest	570:575	arg1	rate					577:580	a lowest rate	568:580	a lowest rate of weight loss	568:595	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	65	theme	lowest	570:575	arg1	%					606:606	about 68%	598:606	about 68% of the weight retained within 18 d in Collagenase IA solution	598:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	6	66	theme	SF/SA	1434:1438	arg1	PMs					1450:1452	the SF/SA composite PMs	1430:1452	the SF/SA composite PMs	1430:1452	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	1	67	theme	/sodium	119:125	arg1	PMs					159:161	PMs	159:161	PMs	159:161	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	67	theme	/sodium	119:125	arg1	materials					148:156	silk fibroin (SF)/sodium alginate (SA) porous materials	102:156	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	5	68	theme	subcutaneous	1155:1166	arg1	histocompatibility					1168:1185	good subcutaneous histocompatibility	1150:1185	good subcutaneous histocompatibility to the host animals	1150:1205	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	5	69	from	implantation	1058:1069	arg1	rats					1074:1077	rats	1074:1077	rats	1074:1077	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31747567	6	70	theme	blend	1257:1261	arg1	ratios					1263:1268	the blend ratios	1253:1268	the blend ratios in a series of SF/SA composite PMs	1253:1303	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	1	71	theme	alginate	127:134	arg1	PMs					159:161	PMs	159:161	PMs	159:161	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	71	theme	alginate	127:134	arg1	materials					148:156	silk fibroin (SF)/sodium alginate (SA) porous materials	102:156	silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios	102:190	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	72	theme	3-dimethylaminopropyl	224:244	arg1	EDC					261:263	EDC	261:263	EDC	261:263	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	1	72	theme	3-dimethylaminopropyl	224:244	arg1	carbodiimide					247:258	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	213:258	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)	213:264	In this study, silk fibroin (SF)/sodium alginate (SA) porous materials (PMs) with different blend ratios were generated using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as crosslinking agent by a simple freeze-dried method.
31747567	3	73	theme	weight	615:620	arg1	weight					615:620	the weight	611:620	the weight retained within 18 d in Collagenase IA solution	611:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	73	theme	weight	615:620	arg1	rate					577:580	a lowest rate	568:580	a lowest rate of weight loss	568:595	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	3	73	theme	weight	615:620	arg1	%					606:606	about 68%	598:606	about 68% of the weight retained within 18 d in Collagenase IA solution	598:668	The results showed SF/SA 50/50 PMs exhibited a lowest rate of weight loss, about 68% of the weight retained within 18 d in Collagenase IA solution.
31747567	6	74	theme	PMs	1301:1303	arg1	series					1275:1280	a series	1273:1280	a series of SF/SA composite PMs	1273:1303	The degradability was strongly correlated to the blend ratios in a series of SF/SA composite PMs, and insights gained in this study can serve as a guide to match desired degradation behavior with specific applications for the SF/SA composite PMs.
31747567	0	75	with	materials	46:54	arg1	degradation					74:84	controllable degradation	61:84	controllable degradation	61:84	Silk fibroin/sodium alginate composite porous materials with controllable degradation.
31747567	2	76	theme	IA	424:425	arg1	solution					476:483	Phosphate buffer saline (PBS) solution	446:483	Phosphate buffer saline (PBS) solution without enzyme	446:498	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	2	76	theme	IA	424:425	arg1	solution					427:434	Collagenase IA solution	412:434	Collagenase IA solution at 37 °C	412:443	Degradation experiment of SF/SA PMs have been systematically investigated up to 18 days in Collagenase IA solution at 37 °C, Phosphate buffer saline (PBS) solution without enzyme was used as a control.
31747567	5	77	theme	different	1118:1126	arg1	levels					1128:1133	different levels	1118:1133	different levels	1118:1133	The crystalline structure of the SF in the SF/SA PMs changed to silk II after degradation for 18 d. Furthermore, the results of the in vivo degradation by subcutaneous implantation in rats showed that all PMs can be degraded at different levels, and exhibited good subcutaneous histocompatibility to the host animals.
31359156	6	0	theme	tablet	856:861	arg1	size					863:866	tablet size	856:866	tablet size	856:866	RESULTS The minimum IBS depends on both layer composition and tablet size.
31359156	10	1	theme	inadequate	1328:1337	arg1	IBS					1339:1341	inadequate IBS	1328:1341	inadequate IBS	1328:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	4	2	theme	drops	670:674	arg1	number					660:665	the number	656:665	the number of drops	656:674	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	0	3	theme	Survival	76:83	arg1	Test					85:88	a Survival Test	74:88	a Survival Test	74:88	Minimum Interfacial Bonding Strength for Bilayer Tablets Determined Using a Survival Test.
31359156	9	4	theme	0.26 MPa	1066:1073	arg1	sufficient					1078:1087	sufficient	1078:1087	sufficient	1078:1087	An IBS of 0.26 MPa is sufficient for all bilayer tablet formulations and sizes to pass the survival test in this work.
31359156	9	4	theme	0.26 MPa	1066:1073	arg1	IBS					1059:1061	An IBS	1056:1061	An IBS of 0.26 MPa	1056:1073	An IBS of 0.26 MPa is sufficient for all bilayer tablet formulations and sizes to pass the survival test in this work.
31359156	10	5	theme	quality	1300:1306	arg1	issues					1308:1313	quality issues	1300:1313	quality issues arising from inadequate IBS	1300:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	6	theme	bilayer	1257:1263	arg1	tablets					1265:1271	bilayer tablets	1257:1271	bilayer tablets of most materials	1257:1289	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	7	7	theme	brittle	904:910	arg1	materials					912:920	more brittle materials	899:920	more brittle materials	899:920	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	3	8	theme	interface	522:530	arg1	integrity					505:513	integrity	505:513	integrity of the interface	505:530	Five bilayer tablets with the same IBS were repeatedly dropped at a fixed height in a friabilator and integrity of the interface was periodically examined.
31359156	4	9	theme	free	581:584	arg1	tablets					573:579	tablets	573:579	tablets free from observable defects at the interface	573:625	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	6	10	theme	layer	834:838	arg1	composition					840:850	layer composition	834:850	layer composition	834:850	RESULTS The minimum IBS depends on both layer composition and tablet size.
31359156	2	11	theme	Bilayer	283:289	arg1	tablets					291:297	Bilayer tablets	283:297	METHODS Bilayer tablets of a number of formulations with systematically varied IBS	275:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	2	12	theme	formulations	314:325	arg1	number					304:309	a number	302:309	a number of formulations with systematically varied IBS	302:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	5	13	theme	given	775:779	arg1	formulation					781:791	a given formulation	773:791	a given formulation	773:791	The IBS for all five tablets to remain intact after 1000 drops was taken as the minimum IBS for a given formulation.
31359156	2	14	theme	materials	377:385	arg1	macine					395:400	a materials testing macine	375:400	a materials testing macine	375:400	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	3	15	theme	same	433:436	arg1	IBS					438:440	the same IBS	429:440	the same IBS	429:440	Five bilayer tablets with the same IBS were repeatedly dropped at a fixed height in a friabilator and integrity of the interface was periodically examined.
31359156	4	16	theme	tablets	573:579	arg1	number					563:568	The number	559:568	The number of tablets free from observable defects at the interface	559:625	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	4	16	theme	tablets	573:579	arg1	function					644:651	a function	642:651	a function of the number of drops	642:674	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	0	17	theme	Bilayer	41:47	arg1	Tablets					49:55	Bilayer Tablets	41:55	Bilayer Tablets	41:55	Minimum Interfacial Bonding Strength for Bilayer Tablets Determined Using a Survival Test.
31359156	7	18	theme	bilayer	873:879	arg1	tablets					881:887	bilayer tablets	873:887	bilayer tablets made with more brittle materials	873:920	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	3	19	with	tablets	416:422	arg1	IBS					438:440	the same IBS	429:440	the same IBS	429:440	Five bilayer tablets with the same IBS were repeatedly dropped at a fixed height in a friabilator and integrity of the interface was periodically examined.
31359156	2	20	theme	testing	387:393	arg1	macine					395:400	a materials testing macine	375:400	a materials testing macine	375:400	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	0	21	theme	Interfacial	8:18	arg1	Strength					28:35	Minimum Interfacial Bonding Strength	0:35	Minimum Interfacial Bonding Strength for Bilayer Tablets	0:55	Minimum Interfacial Bonding Strength for Bilayer Tablets Determined Using a Survival Test.
31359156	8	22	theme	minimum	1043:1049	arg1	IBS					1051:1053	a lower minimum IBS	1035:1053	a lower minimum IBS	1035:1053	The incorporation of HPMC leads to a lower minimum IBS.
31359156	9	23	theme	bilayer	1097:1103	arg1	formulations					1112:1123	all bilayer tablet formulations	1093:1123	all bilayer tablet formulations	1093:1123	An IBS of 0.26 MPa is sufficient for all bilayer tablet formulations and sizes to pass the survival test in this work.
31359156	1	24	theme	bilayer	171:177	arg1	tablets					179:185	bilayer tablets	171:185	bilayer tablets	171:185	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	0	25	theme	Minimum	0:6	arg1	Strength					28:35	Minimum Interfacial Bonding Strength	0:35	Minimum Interfacial Bonding Strength for Bilayer Tablets	0:55	Minimum Interfacial Bonding Strength for Bilayer Tablets Determined Using a Survival Test.
31359156	9	26	theme	tablet	1105:1110	arg1	formulations					1112:1123	all bilayer tablet formulations	1093:1123	all bilayer tablet formulations	1093:1123	An IBS of 0.26 MPa is sufficient for all bilayer tablet formulations and sizes to pass the survival test in this work.
31359156	2	27	theme	number	304:309	arg1	tablets					291:297	Bilayer tablets	283:297	METHODS Bilayer tablets of a number of formulations with systematically varied IBS	275:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	6	28	theme	minimum	806:812	arg1	IBS					814:816	The minimum IBS	802:816	The minimum IBS	802:816	RESULTS The minimum IBS depends on both layer composition and tablet size.
31359156	5	29	theme	minimum	757:763	arg1	IBS					681:683	The IBS	677:683	The IBS for all five tablets to remain intact after 1000 drops	677:738	The IBS for all five tablets to remain intact after 1000 drops was taken as the minimum IBS for a given formulation.
31359156	5	29	theme	minimum	757:763	arg1	IBS					765:767	the minimum IBS	753:767	the minimum IBS for a given formulation	753:791	The IBS for all five tablets to remain intact after 1000 drops was taken as the minimum IBS for a given formulation.
31359156	10	30	theme	materials	1281:1289	arg1	tablets					1265:1271	bilayer tablets	1257:1271	bilayer tablets of most materials	1257:1289	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	31	theme	minimum	1189:1195	arg1	criterion					1243:1251	a tentative criterion	1231:1251	a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS	1231:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	31	theme	minimum	1189:1195	arg1	IBS					1197:1199	A minimum IBS	1187:1199	A minimum IBS of 0.26 MPa	1187:1211	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	32	theme	most	1276:1279	arg1	materials					1281:1289	most materials	1276:1289	most materials	1276:1289	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	0	33	theme	Bonding	20:26	arg1	Strength					28:35	Minimum Interfacial Bonding Strength	0:35	Minimum Interfacial Bonding Strength for Bilayer Tablets	0:55	Minimum Interfacial Bonding Strength for Bilayer Tablets Determined Using a Survival Test.
31359156	7	34	theme	survival	985:992	arg1	test					994:997	the survival test	981:997	the survival test	981:997	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	4	35	from	interface	617:625	arg1	defects					602:608	observable defects	591:608	observable defects at the interface	591:625	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	4	36	theme	number	660:665	arg1	number					563:568	The number	559:568	The number of tablets free from observable defects at the interface	559:625	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	4	36	theme	number	660:665	arg1	function					644:651	a function	642:651	a function of the number of drops	642:674	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	3	37	theme	bilayer	408:414	arg1	tablets					416:422	Five bilayer tablets	403:422	Five bilayer tablets with the same IBS	403:440	Five bilayer tablets with the same IBS were repeatedly dropped at a fixed height in a friabilator and integrity of the interface was periodically examined.
31359156	2	38	with	formulations	314:325	arg1	IBS					354:356	systematically varied IBS	332:356	systematically varied IBS	332:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	6	39	dep	RESULTS	794:800	arg1	depends					818:824	depends	818:824	depends on both layer composition and tablet size	818:866	RESULTS The minimum IBS depends on both layer composition and tablet size.
31359156	8	40	theme	HPMC	1021:1024	arg1	incorporation					1004:1016	The incorporation	1000:1016	The incorporation of HPMC	1000:1024	The incorporation of HPMC leads to a lower minimum IBS.
31359156	7	41	theme	higher	942:947	arg1	IBS					957:959	a higher minimum IBS	940:959	a higher minimum IBS	940:959	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	2	42	theme	varied	347:352	arg1	IBS					354:356	systematically varied IBS	332:356	systematically varied IBS	332:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	1	43	theme	minimum	115:121	arg1	IBS					153:155	IBS	153:155	IBS	153:155	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	1	43	theme	minimum	115:121	arg1	strength					143:150	the minimum interfacial bonding strength	111:150	the minimum interfacial bonding strength (IBS)	111:156	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	7	44	theme	minimum	949:955	arg1	IBS					957:959	a higher minimum IBS	940:959	a higher minimum IBS	940:959	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	9	45	theme	survival	1147:1154	arg1	test					1156:1159	the survival test	1143:1159	the survival test	1143:1159	An IBS of 0.26 MPa is sufficient for all bilayer tablet formulations and sizes to pass the survival test in this work.
31359156	10	46	theme	0.26 MPa	1204:1211	arg1	criterion					1243:1251	a tentative criterion	1231:1251	a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS	1231:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	46	theme	0.26 MPa	1204:1211	arg1	IBS					1197:1199	A minimum IBS	1187:1199	A minimum IBS of 0.26 MPa	1187:1211	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	1	47	theme	interfacial	123:133	arg1	IBS					153:155	IBS	153:155	IBS	153:155	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	1	47	theme	interfacial	123:133	arg1	strength					143:150	the minimum interfacial bonding strength	111:150	the minimum interfacial bonding strength (IBS)	111:156	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	10	48	dep	CONCLUSIONS	1175:1185	arg1	recommended					1216:1226	recommended	1216:1226	is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS	1213:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	4	49	theme	observable	591:600	arg1	defects					602:608	observable defects	591:608	observable defects at the interface	591:625	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	1	50	theme	bonding	135:141	arg1	IBS					153:155	IBS	153:155	IBS	153:155	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	1	50	theme	bonding	135:141	arg1	strength					143:150	the minimum interfacial bonding strength	111:150	the minimum interfacial bonding strength (IBS)	111:156	PURPOSE To identify the minimum interfacial bonding strength (IBS) required for bilayer tablets to sustain the stresses experienced during manufacturing, transportation, and handling.
31359156	7	51	theme	larger	927:932	arg1	size					934:937	a larger size	925:937	a larger size	925:937	For bilayer tablets made with more brittle materials or a larger size, a higher minimum IBS is required to pass the survival test.
31359156	2	52	dep	METHODS	275:281	arg1	tablets					291:297	Bilayer tablets	283:297	METHODS Bilayer tablets of a number of formulations with systematically varied IBS	275:356	METHODS Bilayer tablets of a number of formulations with systematically varied IBS were prepared on a materials testing macine.
31359156	3	53	theme	fixed	471:475	arg1	height					477:482	a fixed height	469:482	a fixed height	469:482	Five bilayer tablets with the same IBS were repeatedly dropped at a fixed height in a friabilator and integrity of the interface was periodically examined.
31359156	4	54	from	defects	602:608	arg1	free					581:584	free	581:584	free	581:584	The number of tablets free from observable defects at the interface was plotted as a function of the number of drops.
31359156	10	55	theme	tentative	1233:1241	arg1	IBS					1197:1199	A minimum IBS	1187:1199	A minimum IBS of 0.26 MPa	1187:1211	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31359156	10	55	theme	tentative	1233:1241	arg1	criterion					1243:1251	a tentative criterion	1231:1251	a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS	1231:1341	CONCLUSIONS A minimum IBS of 0.26 MPa is recommended as a tentative criterion for bilayer tablets of most materials to avoid quality issues arising from inadequate IBS.
31018777	4	0	theme	prepared	707:714	arg1	composite					726:734	The prepared CRC/AgNPs composite	703:734	The prepared CRC/AgNPs composite	703:734	The prepared CRC/AgNPs composite was characterized using XRD, FTIR and SEM techniques.
31018777	2	1	theme	CRC/AgNPs	396:404	arg1	Ag-nanocomposites					377:393	cellulosic Ag-nanocomposites	366:393	cellulosic Ag-nanocomposites (CRC/AgNPs composite)	366:415	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	2	1	theme	CRC/AgNPs	396:404	arg1	composite					406:414	CRC/AgNPs composite	396:414	CRC/AgNPs composite	396:414	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	8	2	theme	CRC/AgNPs	1118:1126	arg1	composite					1128:1136	CRC/AgNPs composite	1118:1136	CRC/AgNPs composite	1118:1136	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite was observed in comparison to CRC.
31018777	3	3	theme	composite	692:700	arg1	preparation					667:677	preparation	667:677	preparation of CRC/AgNPs composite	667:700	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	10	4	theme	materials	1413:1421	arg1	strength					1391:1398	the compression strength	1375:1398	the compression strength of polymeric materials	1375:1421	Moreover, the addition of CRC/AgNPs composite as a filler increased the compression strength of polymeric materials.
31018777	10	5	theme	polymeric	1403:1411	arg1	materials					1413:1421	polymeric materials	1403:1421	polymeric materials	1403:1421	Moreover, the addition of CRC/AgNPs composite as a filler increased the compression strength of polymeric materials.
31018777	4	6	theme	SEM	774:776	arg1	techniques					778:787	XRD, FTIR and SEM techniques	760:787	XRD, FTIR and SEM techniques	760:787	The prepared CRC/AgNPs composite was characterized using XRD, FTIR and SEM techniques.
31018777	1	7	theme	Cellulose-based	67:81	arg1	nanocomposites					83:96	Cellulose-based nanocomposites	67:96	Cellulose-based nanocomposites	67:96	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	3	8	theme	hemicellulose	528:540	arg1	components					542:551	removing lignin and hemicellulose components	508:551	components	542:551	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	9	theme	removing	508:515	arg1	lignin					517:522	removing lignin and hemicellulose components	508:551	lignin	517:522	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	2	10	theme	Neem	422:425	arg1	plant					427:431	Neem plant	422:431	Neem plant	422:431	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	5	11	theme	cellulosic	869:878	arg1	constituent					880:890	cellulosic constituent	869:890	cellulosic constituent	869:890	The XRD and FTIR spectral analysis showed the characteristic peaks assigned to cellulosic constituent and AgNPs.
31018777	6	12	theme	SEM	903:905	arg1	analysis					907:914	SEM analysis	903:914	SEM analysis	903:914	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	8	13	theme	antibacterial	1061:1073	arg1	activity					1075:1082	The antibacterial activity	1057:1082	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite	1057:1136	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite was observed in comparison to CRC.
31018777	2	14	theme	liquid	303:308	arg1	solvent					339:345	an efficient solvent	326:345	an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant	326:431	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	2	14	theme	liquid	303:308	arg1	1-h-3-methylimidazolium					256:278	1-h-3-methylimidazolium	256:278	1-h-3-methylimidazolium hydrogen sulfate ionic liquid	256:308	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	5	15	theme	spectral	807:814	arg1	analysis					816:823	The XRD and FTIR spectral analysis	790:823	analysis	816:823	The XRD and FTIR spectral analysis showed the characteristic peaks assigned to cellulosic constituent and AgNPs.
31018777	9	16	theme	MC3T3-E1	1221:1228	arg1	cells					1230:1234	MC3T3-E1 cells	1221:1234	MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite	1221:1304	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	4	17	theme	XRD	760:762	arg1	techniques					778:787	XRD, FTIR and SEM techniques	760:787	XRD, FTIR and SEM techniques	760:787	The prepared CRC/AgNPs composite was characterized using XRD, FTIR and SEM techniques.
31018777	3	18	theme	dual	455:458	arg1	role					460:463	a dual role	453:463	a dual role	453:463	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	1	19	theme	much	110:113	arg1	attention					115:123	much attention	110:123	much attention	110:123	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	0	20	theme	Ag-nanocomposites	26:42	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulosic Ag-nanocomposites using an ionic liquid.	0:65	Preparation of cellulosic Ag-nanocomposites using an ionic liquid.
31018777	6	21	from	nm	966:967	arg1	particles					929:937	the particles	925:937	the particles in the range from 26 to 56 nm	925:967	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	6	21	from	nm	966:967	arg1	range					946:950	the range	942:950	the range from 26 to 56 nm	942:967	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	3	22	theme	phenolic	575:582	arg1	tannins					614:620	tannins	614:620	tannins	614:620	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	22	theme	phenolic	575:582	arg1	compounds					584:592	phenolic compounds	575:592	phenolic compounds such as flavonoids	575:611	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	22	theme	phenolic	575:582	arg1	flavonoids					602:611	flavonoids	602:611	flavonoids	602:611	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	22	theme	phenolic	575:582	arg1	etc.					623:626	etc.	623:626	etc.	623:626	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	1	23	theme	low	219:221	arg1	toxicity					223:230	low toxicity	219:230	low toxicity	219:230	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	0	24	theme	cellulosic	15:24	arg1	Ag-nanocomposites					26:42	cellulosic Ag-nanocomposites	15:42	cellulosic Ag-nanocomposites	15:42	Preparation of cellulosic Ag-nanocomposites using an ionic liquid.
31018777	4	25	theme	CRC/AgNPs	716:724	arg1	composite					726:734	The prepared CRC/AgNPs composite	703:734	The prepared CRC/AgNPs composite	703:734	The prepared CRC/AgNPs composite was characterized using XRD, FTIR and SEM techniques.
31018777	3	26	theme	cellulose-rich	478:491	arg1	compound					493:500	cellulose-rich compound	478:500	cellulose-rich compound (CRC; removing lignin and hemicellulose components)	478:552	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	9	27	theme	as	1252:1253	arg1	toxicity					1260:1267	no as such toxicity	1249:1267	no as such toxicity	1249:1267	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	7	28	theme	antibacterial	1016:1028	arg1	properties					1045:1054	its antibacterial and mechanical properties	1012:1054	its antibacterial and mechanical properties	1012:1054	The CRC/AgNPs composite was evaluated for its antibacterial and mechanical properties.
31018777	4	29	theme	FTIR	765:768	arg1	techniques					778:787	XRD, FTIR and SEM techniques	760:787	XRD, FTIR and SEM techniques	760:787	The prepared CRC/AgNPs composite was characterized using XRD, FTIR and SEM techniques.
31018777	8	30	located	observed	1142:1149	arg1	comparison					1154:1163	comparison	1154:1163	comparison to CRC	1154:1170	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite was observed in comparison to CRC.
31018777	8	30	located	observed	1142:1149	arg2	activity					1075:1082	The antibacterial activity	1057:1082	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite	1057:1136	The antibacterial activity against S. aureus and E. coli for CRC/AgNPs composite was observed in comparison to CRC.
31018777	9	31	theme	such	1255:1258	arg1	toxicity					1260:1267	no as such toxicity	1249:1267	no as such toxicity	1249:1267	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	2	32	theme	research	241:248	arg1	work					250:253	this research work	236:253	this research work	236:253	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	2	33	theme	Ag-nanocomposites	377:393	arg1	preparation					351:361	preparation	351:361	preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant	351:431	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	5	34	theme	FTIR	802:805	arg1	analysis					816:823	The XRD and FTIR spectral analysis	790:823	analysis	816:823	The XRD and FTIR spectral analysis showed the characteristic peaks assigned to cellulosic constituent and AgNPs.
31018777	6	35	dep	56	963:964	arg1	to					960:961	to	960:961	to	960:961	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	2	36	theme	cellulosic	366:375	arg1	Ag-nanocomposites					377:393	cellulosic Ag-nanocomposites	366:393	cellulosic Ag-nanocomposites (CRC/AgNPs composite)	366:415	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	2	36	theme	cellulosic	366:375	arg1	composite					406:414	CRC/AgNPs composite	396:414	CRC/AgNPs composite	396:414	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	9	37	theme	Cell	1173:1176	arg1	viability					1178:1186	Cell viability	1173:1186	Cell viability	1173:1186	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	7	38	theme	mechanical	1034:1043	arg1	properties					1045:1054	its antibacterial and mechanical properties	1012:1054	its antibacterial and mechanical properties	1012:1054	The CRC/AgNPs composite was evaluated for its antibacterial and mechanical properties.
31018777	3	39	theme	CRC/AgNPs	682:690	arg1	composite					692:700	CRC/AgNPs composite	682:700	CRC/AgNPs composite	682:700	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	2	40	from	plant	427:431	arg1	preparation					351:361	preparation	351:361	preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant	351:431	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	1	41	theme	remarkable	138:147	arg1	toxicity					223:230	low toxicity	219:230	low toxicity	219:230	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	41	theme	remarkable	138:147	arg1	biocompatibility					197:212	biocompatibility	197:212	biocompatibility	197:212	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	41	theme	remarkable	138:147	arg1	properties					160:169	their remarkable biological properties	132:169	their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity	132:230	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	41	theme	remarkable	138:147	arg1	biodegradability					179:194	biodegradability	179:194	biodegradability	179:194	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	2	42	theme	efficient	329:337	arg1	solvent					339:345	an efficient solvent	326:345	an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant	326:431	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	2	42	theme	efficient	329:337	arg1	1-h-3-methylimidazolium					256:278	1-h-3-methylimidazolium	256:278	1-h-3-methylimidazolium hydrogen sulfate ionic liquid	256:308	In this research work, 1-h-3-methylimidazolium hydrogen sulfate ionic liquid was employed as an efficient solvent for preparation of cellulosic Ag-nanocomposites (CRC/AgNPs composite) from Neem plant.
31018777	10	43	theme	CRC/AgNPs	1333:1341	arg1	addition					1321:1328	the addition	1317:1328	the addition of CRC/AgNPs composite as a filler	1317:1363	Moreover, the addition of CRC/AgNPs composite as a filler increased the compression strength of polymeric materials.
31018777	3	44	dep	compound	493:500	arg1	CRC					503:505	CRC	503:505	CRC; removing lignin and hemicellulose components	503:551	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	10	45	theme	compression	1379:1389	arg1	strength					1391:1398	the compression strength	1375:1398	the compression strength of polymeric materials	1375:1421	Moreover, the addition of CRC/AgNPs composite as a filler increased the compression strength of polymeric materials.
31018777	1	46	theme	biological	149:158	arg1	toxicity					223:230	low toxicity	219:230	low toxicity	219:230	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	46	theme	biological	149:158	arg1	biocompatibility					197:212	biocompatibility	197:212	biocompatibility	197:212	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	46	theme	biological	149:158	arg1	properties					160:169	their remarkable biological properties	132:169	their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity	132:230	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	1	46	theme	biological	149:158	arg1	biodegradability					179:194	biodegradability	179:194	biodegradability	179:194	Cellulose-based nanocomposites have gained much attention due to their remarkable biological properties such as biodegradability, biocompatibility, and low toxicity.
31018777	3	47	dep	extract	566:572	arg1	tannins					614:620	tannins	614:620	tannins	614:620	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	47	dep	extract	566:572	arg1	compounds					584:592	phenolic compounds	575:592	phenolic compounds such as flavonoids	575:611	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	47	dep	extract	566:572	arg1	flavonoids					602:611	flavonoids	602:611	flavonoids	602:611	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	47	dep	extract	566:572	arg1	etc.					623:626	etc.	623:626	etc.	623:626	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	6	48	from	particles	929:937	arg1	nm					966:967	26 to 56 nm	957:967	26 to 56 nm	957:967	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	6	48	from	particles	929:937	arg1	range					946:950	the range	942:950	the range from 26 to 56 nm	942:967	SEM analysis revealed the particles in the range from 26 to 56 nm.
31018777	9	49	theme	prepared	1277:1284	arg1	composite					1296:1304	the prepared CRC/AgNPs composite	1273:1304	the prepared CRC/AgNPs composite	1273:1304	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	5	50	theme	characteristic	836:849	arg1	peaks					851:855	the characteristic peaks	832:855	the characteristic peaks assigned to cellulosic constituent and AgNPs	832:900	The XRD and FTIR spectral analysis showed the characteristic peaks assigned to cellulosic constituent and AgNPs.
31018777	9	51	theme	CRC/AgNPs	1286:1294	arg1	composite					1296:1304	the prepared CRC/AgNPs composite	1273:1304	the prepared CRC/AgNPs composite	1273:1304	Cell viability and morphology were performed on MC3T3-E1 cells which showed no as such toxicity for the prepared CRC/AgNPs composite.
31018777	3	52	dep	CRC	503:505	arg1	lignin					517:522	removing lignin and hemicellulose components	508:551	lignin	517:522	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	3	52	dep	CRC	503:505	arg1	components					542:551	removing lignin and hemicellulose components	508:551	components	542:551	Ionic liquid plays a dual role in obtaining cellulose-rich compound (CRC; removing lignin and hemicellulose components) and plant's extract (phenolic compounds such as flavonoids, tannins, etc.) that reduces the AgNO3 into AgNPs for preparation of CRC/AgNPs composite.
31018777	7	53	theme	CRC/AgNPs	974:982	arg1	composite					984:992	The CRC/AgNPs composite	970:992	The CRC/AgNPs composite	970:992	The CRC/AgNPs composite was evaluated for its antibacterial and mechanical properties.
29690771	2	0	theme	HWB	600:602	arg1	regions					604:610	LWB and HWB regions	592:610	LWB and HWB regions	592:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	9	1	theme	tissues	1630:1636	arg1	properties					1616:1625	The properties	1612:1625	The properties of tissues from LWB regions	1612:1653	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	4	2	theme	MIR	806:808	arg1	spectra					823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra	768:829	spectra	823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	2	3	theme	LWB	592:594	arg1	regions					604:610	LWB and HWB regions	592:610	LWB and HWB regions	592:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	3	4	theme	bovine	696:701	arg1	joints					710:715	juvenile bovine stifle joints	687:715	juvenile bovine stifle joints	687:715	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	6	5	theme	HWB	1203:1205	arg1	cartilage					1215:1223	HWB condyle cartilage	1203:1223	HWB condyle cartilage having significantly higher moduli	1203:1258	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	7	6	located	found	1311:1315	arg2	amount					1271:1276	A greater amount	1261:1276	A greater amount of proteoglycan content	1261:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	7	6	located	found	1311:1315	arg2	content					1294:1300	proteoglycan content	1281:1300	proteoglycan content	1281:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	7	6	located	found	1311:1315	arg1	tissues					1324:1330	HWB tissues	1320:1330	HWB tissues	1320:1330	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	10	7	theme	osteochondral	1834:1846	arg1	grafts					1848:1853	harvesting osteochondral grafts	1823:1853	harvesting osteochondral grafts	1823:1853	Clinicians may be able to use this modality to assess AC prior to harvesting osteochondral grafts for focal defect repair.
29690771	1	8	theme	repair	191:196	arg1	procedures					198:207	repair procedures	191:207	repair procedures of high weight bearing (HWB) regions of the femoral condyles	191:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	4	9	theme	mid-infrared	792:803	arg1	spectra					823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra	768:829	spectra	823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	3	10	theme	joints	710:715	arg1	groove					677:682	the patellofemoral groove	658:682	the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region	658:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	1	11	theme	weight	217:222	arg1	HWB					233:235	HWB	233:235	HWB	233:235	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	11	theme	weight	217:222	arg1	bearing					224:230	high weight bearing	212:230	high weight bearing (HWB) regions of the femoral condyles	212:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	12	theme	Autologous	141:150	arg1	AC					173:174	AC	173:174	AC	173:174	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	12	theme	Autologous	141:150	arg1	cartilage					162:170	OBJECTIVE Autologous articular cartilage	131:170	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles	131:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	7	13	theme	HWB	1320:1322	arg1	tissues					1324:1330	HWB tissues	1320:1330	HWB tissues	1320:1330	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	1	14	theme	femoral	253:259	arg1	condyles					261:268	the femoral condyles	249:268	the femoral condyles	249:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	15	theme	non-destructive	358:372	arg1	techniques					374:383	non-destructive techniques	358:383	non-destructive techniques for cartilage composition assessment	358:420	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	2	16	theme	AC	582:583	arg1	properties					568:577	compositional and mechanical properties	539:577	compositional and mechanical properties of AC across LWB and HWB regions	539:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	6	17	theme	patellofemoral	1159:1172	arg1	groove					1174:1179	the patellofemoral groove	1155:1179	the patellofemoral groove to the condyles	1155:1195	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	3	18	theme	region	724:729	arg1	groove					677:682	the patellofemoral groove	658:682	the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region	658:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	4	19	theme	fiber	811:815	arg1	spectra					823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra	768:829	spectra	823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	9	20	theme	LWB	1643:1645	arg1	regions					1647:1653	LWB regions	1643:1653	LWB regions	1643:1653	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	1	21	theme	composition	399:409	arg1	assessment					411:420	cartilage composition assessment	389:420	cartilage composition assessment	389:420	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	3	22	theme	AC	620:621	arg1	N					630:630	N = 72	630:635	N = 72	630:635	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	3	22	theme	AC	620:621	arg1	plugs					623:627	DESIGN AC plugs	613:627	DESIGN AC plugs (N = 72)	613:636	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	9	23	from	regions	1647:1653	arg1	properties					1616:1625	The properties	1612:1625	The properties of tissues from LWB regions	1612:1653	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	9	23	from	regions	1647:1653	arg1	tissues					1630:1636	tissues	1630:1636	tissues from LWB regions	1630:1653	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	7	24	theme	proteoglycan	1281:1292	arg1	content					1294:1300	proteoglycan content	1281:1300	proteoglycan content	1281:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	6	25	from	groove	1174:1179	arg1	tissues					1142:1148	tissues	1142:1148	tissues from the patellofemoral groove to the condyles	1142:1195	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	3	26	theme	condyles	744:751	arg1	groove					677:682	the patellofemoral groove	658:682	the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region	658:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	0	27	theme	Bearing	64:70	arg1	Cartilage					82:90	High and Low Weight Bearing Articular Cartilage	44:90	High and Low Weight Bearing Articular Cartilage	44:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	9	28	theme	fiber	1731:1735	arg1	spectroscopy					1743:1754	infrared fiber optic spectroscopy	1722:1754	infrared fiber optic spectroscopy	1722:1754	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	9	29	theme	HWB	1667:1669	arg1	tissues					1671:1677	HWB tissues	1667:1677	HWB tissues	1667:1677	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	0	30	theme	Cartilage	82:90	arg1	Assessment					30:39	Non-Destructive Spectroscopic Assessment	0:39	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage	0:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	8	31	theme	proteoglycan	1446:1457	arg1	content					1459:1465	proteoglycan content	1446:1465	proteoglycan content	1446:1465	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	3	32	theme	region	760:765	arg1	groove					677:682	the patellofemoral groove	658:682	the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region	658:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	5	33	theme	greater	1043:1049	arg1	water					1061:1065	water	1061:1065	water	1061:1065	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	5	33	theme	greater	1043:1049	arg1	amount					1051:1056	a significantly greater amount	1027:1056	a significantly greater amount of water determined by NIR and gravimetric assay	1027:1105	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	2	34	theme	fiber	458:462	arg1	spectroscopy					470:481	infrared fiber optic spectroscopy	449:481	infrared fiber optic spectroscopy	449:481	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	5	35	contain	had	1023:1025	arg2	amount					1051:1056	a significantly greater amount	1027:1056	a significantly greater amount of water determined by NIR and gravimetric assay	1027:1105	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	5	35	contain	had	1023:1025	arg2	water					1061:1065	water	1061:1065	water	1061:1065	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	5	35	contain	had	1023:1025	arg1	tissues					1015:1021	tissues	1015:1021	tissues	1015:1021	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	0	36	theme	Non-Destructive	0:14	arg1	Assessment					30:39	Non-Destructive Spectroscopic Assessment	0:39	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage	0:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	7	37	theme	greater	1263:1269	arg1	amount					1271:1276	A greater amount	1261:1276	A greater amount of proteoglycan content	1261:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	7	37	theme	greater	1263:1269	arg1	content					1294:1300	proteoglycan content	1281:1300	proteoglycan content	1281:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	9	38	theme	infrared	1722:1729	arg1	spectroscopy					1743:1754	infrared fiber optic spectroscopy	1722:1754	infrared fiber optic spectroscopy	1722:1754	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	4	39	theme	equilibrium	927:937	arg1	moduli					939:944	equilibrium moduli	927:944	equilibrium moduli	927:944	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	3	40	theme	=	632:632	arg1	N					630:630	N = 72	630:635	N = 72	630:635	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	3	40	theme	=	632:632	arg1	plugs					623:627	DESIGN AC plugs	613:627	DESIGN AC plugs (N = 72)	613:636	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	0	41	theme	High	44:47	arg1	Cartilage					82:90	High and Low Weight Bearing Articular Cartilage	44:90	High and Low Weight Bearing Articular Cartilage	44:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	1	42	theme	weight	301:306	arg1	bearing					308:314	low weight bearing	297:314	low weight bearing (LWB) regions	297:328	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	42	theme	weight	301:306	arg1	LWB					317:319	LWB	317:319	LWB	317:319	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	0	43	theme	Low	53:55	arg1	Cartilage					82:90	High and Low Weight Bearing Articular Cartilage	44:90	High and Low Weight Bearing Articular Cartilage	44:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	5	44	theme	gravimetric	1089:1099	arg1	assay					1101:1105	gravimetric assay	1089:1105	gravimetric assay	1089:1105	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	8	45	theme	multivariate	1510:1521	arg1	able					1547:1550	able	1547:1550	able	1547:1550	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	8	45	theme	multivariate	1510:1521	arg1	model					1523:1527	a multivariate model	1508:1527	a multivariate model with NIR data	1508:1541	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	1	46	theme	regions	238:244	arg1	procedures					198:207	repair procedures	191:207	repair procedures of high weight bearing (HWB) regions of the femoral condyles	191:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	4	47	theme	biochemical	981:991	arg1	analysis					993:1000	biochemical analysis	981:1000	biochemical analysis	981:1000	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	10	48	theme	harvesting	1823:1832	arg1	grafts					1848:1853	harvesting osteochondral grafts	1823:1853	harvesting osteochondral grafts	1823:1853	Clinicians may be able to use this modality to assess AC prior to harvesting osteochondral grafts for focal defect repair.
29690771	8	49	theme	15	1589:1590	arg1	%					1591:1591	%	1591:1591	%	1591:1591	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	6	50	theme	condyle	1207:1213	arg1	cartilage					1215:1223	HWB condyle cartilage	1203:1223	HWB condyle cartilage having significantly higher moduli	1203:1258	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	1	51	theme	condyles	261:268	arg1	regions					238:244	high weight bearing (HWB) regions	212:244	high weight bearing (HWB) regions of the femoral condyles	212:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	10	52	theme	defect	1865:1870	arg1	repair					1872:1877	focal defect repair	1859:1877	focal defect repair	1859:1877	Clinicians may be able to use this modality to assess AC prior to harvesting osteochondral grafts for focal defect repair.
29690771	3	53	theme	stifle	703:708	arg1	joints					710:715	juvenile bovine stifle joints	687:715	juvenile bovine stifle joints	687:715	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	8	54	with	model	1523:1527	arg1	data					1538:1541	NIR data	1534:1541	NIR data	1534:1541	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	7	55	theme	collagen	1355:1362	arg1	content					1364:1370	collagen content	1355:1370	collagen content	1355:1370	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	3	56	theme	juvenile	687:694	arg1	joints					710:715	juvenile bovine stifle joints	687:715	juvenile bovine stifle joints	687:715	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	3	57	theme	LWB	720:722	arg1	region					724:729	a LWB region	718:729	a LWB region	718:729	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	1	58	theme	high	212:215	arg1	HWB					233:235	HWB	233:235	HWB	233:235	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	58	theme	high	212:215	arg1	bearing					224:230	high weight bearing	212:230	high weight bearing (HWB) regions of the femoral condyles	212:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	2	59	theme	compositional	539:551	arg1	properties					568:577	compositional and mechanical properties	539:577	compositional and mechanical properties of AC across LWB and HWB regions	539:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	10	60	theme	focal	1859:1863	arg1	repair					1872:1877	focal defect repair	1859:1877	focal defect repair	1859:1877	Clinicians may be able to use this modality to assess AC prior to harvesting osteochondral grafts for focal defect repair.
29690771	1	61	theme	bearing	224:230	arg1	regions					238:244	high weight bearing (HWB) regions	212:244	high weight bearing (HWB) regions of the femoral condyles	212:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	8	62	theme	NIR-determined	1386:1399	arg1	water					1401:1405	NIR-determined water	1386:1405	NIR-determined water	1386:1405	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	3	63	theme	femoral	736:742	arg1	condyles					744:751	femoral condyles	736:751	femoral condyles	736:751	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	1	64	theme	techniques	374:383	arg1	lack					350:353	the lack	346:353	the lack of non-destructive techniques for cartilage composition assessment	346:420	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	65	theme	OBJECTIVE	131:139	arg1	AC					173:174	AC	173:174	AC	173:174	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	65	theme	OBJECTIVE	131:139	arg1	cartilage					162:170	OBJECTIVE Autologous articular cartilage	131:170	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles	131:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	4	66	theme	optic	817:821	arg1	spectra					823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra	768:829	spectra	823:829	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	6	67	theme	higher	1246:1251	arg1	moduli					1253:1258	significantly higher moduli	1232:1258	significantly higher moduli	1232:1258	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	1	68	theme	cartilage	389:397	arg1	assessment					411:420	cartilage composition assessment	389:420	cartilage composition assessment	389:420	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	3	69	theme	DESIGN	613:618	arg1	N					630:630	N = 72	630:635	N = 72	630:635	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	3	69	theme	DESIGN	613:618	arg1	plugs					623:627	DESIGN AC plugs	613:627	DESIGN AC plugs (N = 72)	613:636	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	1	70	theme	articular	152:160	arg1	AC					173:174	AC	173:174	AC	173:174	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	70	theme	articular	152:160	arg1	cartilage					162:170	OBJECTIVE Autologous articular cartilage	131:170	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles	131:268	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	2	71	theme	mechanical	557:566	arg1	properties					568:577	compositional and mechanical properties	539:577	compositional and mechanical properties of AC across LWB and HWB regions	539:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	8	72	theme	short-term	1423:1432	arg1	modulus					1434:1440	short-term modulus	1423:1440	short-term modulus	1423:1440	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	3	73	theme	HWB	756:758	arg1	region					760:765	a HWB region	754:765	a HWB region	754:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	0	74	theme	Articular	72:80	arg1	Cartilage					82:90	High and Low Weight Bearing Articular Cartilage	44:90	High and Low Weight Bearing Articular Cartilage	44:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	7	75	from	differences	1340:1350	arg1	content					1364:1370	collagen content	1355:1370	collagen content	1355:1370	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	7	76	theme	content	1294:1300	arg1	amount					1271:1276	A greater amount	1261:1276	A greater amount of proteoglycan content	1261:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	7	76	theme	content	1294:1300	arg1	content					1294:1300	proteoglycan content	1281:1300	proteoglycan content	1281:1300	A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content.
29690771	9	77	dep	CONCLUSIONS	1600:1610	arg1	differ					1655:1660	differ	1655:1660	differ from HWB tissues	1655:1677	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	9	77	dep	CONCLUSIONS	1600:1610	arg1	determined					1690:1699	determined	1690:1699	can be determined non-destructively by infrared fiber optic spectroscopy	1683:1754	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	5	78	theme	water	1061:1065	arg1	water					1061:1065	water	1061:1065	water	1061:1065	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	5	78	theme	water	1061:1065	arg1	amount					1051:1056	a significantly greater amount	1027:1056	a significantly greater amount of water determined by NIR and gravimetric assay	1027:1105	RESULTS LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay.
29690771	9	79	from	properties	1616:1625	arg1	regions					1647:1653	LWB regions	1643:1653	LWB regions	1643:1653	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	2	80	theme	optic	464:468	arg1	spectroscopy					470:481	infrared fiber optic spectroscopy	449:481	infrared fiber optic spectroscopy	449:481	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	0	81	theme	Mechanical	108:117	arg1	Properties					119:128	Mechanical Properties	108:128	Mechanical Properties	108:128	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	4	82	theme	indentation	862:872	arg1	tests					874:878	indentation tests	862:878	indentation tests	862:878	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	1	83	theme	due	339:341	arg1	part					334:337	part	334:337	part due to the lack of non-destructive techniques for cartilage composition assessment	334:420	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	2	84	from	variations	525:534	arg1	properties					568:577	compositional and mechanical properties	539:577	compositional and mechanical properties of AC across LWB and HWB regions	539:610	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	0	85	theme	Spectroscopic	16:28	arg1	Assessment					30:39	Non-Destructive Spectroscopic Assessment	0:39	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage	0:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	6	86	contain	having	1225:1230	arg1	cartilage					1215:1223	HWB condyle cartilage	1203:1223	HWB condyle cartilage having significantly higher moduli	1203:1258	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	6	86	contain	having	1225:1230	arg2	moduli					1253:1258	significantly higher moduli	1232:1258	significantly higher moduli	1232:1258	The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli.
29690771	4	87	theme	gravimetric	959:969	arg1	water					971:975	gravimetric water	959:975	gravimetric water	959:975	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	2	88	used	used	490:493	arg2	spectroscopy					470:481	infrared fiber optic spectroscopy	449:481	infrared fiber optic spectroscopy	449:481	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	1	89	theme	low	297:299	arg1	bearing					308:314	low weight bearing	297:314	low weight bearing (LWB) regions	297:328	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	1	89	theme	low	297:299	arg1	LWB					317:319	LWB	317:319	LWB	317:319	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	2	90	theme	infrared	449:456	arg1	spectroscopy					470:481	infrared fiber optic spectroscopy	449:481	infrared fiber optic spectroscopy	449:481	Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions.
29690771	0	91	theme	Weight	57:62	arg1	Bearing					64:70	Weight Bearing	57:70	High and Low Weight Bearing Articular Cartilage	44:90	Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.
29690771	1	92	theme	bearing	308:314	arg1	regions					322:328	low weight bearing (LWB) regions	297:328	low weight bearing (LWB) regions	297:328	OBJECTIVE Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment.
29690771	4	93	theme	Near-infrared	768:780	arg1	NIR					783:785	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra	768:829	NIR	783:785	Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis.
29690771	8	94	theme	short-term	1563:1572	arg1	modulus					1574:1580	short-term modulus	1563:1580	short-term modulus within 15% error	1563:1597	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	9	95	theme	optic	1737:1741	arg1	spectroscopy					1743:1754	infrared fiber optic spectroscopy	1722:1754	infrared fiber optic spectroscopy	1722:1754	CONCLUSIONS The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy.
29690771	8	96	theme	NIR	1534:1536	arg1	data					1538:1541	NIR data	1534:1541	NIR data	1534:1541	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29690771	3	97	theme	patellofemoral	662:675	arg1	groove					677:682	the patellofemoral groove	658:682	the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region	658:765	DESIGN AC plugs (N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region.
29690771	8	98	theme	%	1591:1591	arg1	error					1593:1597	15% error	1589:1597	15% error	1589:1597	In addition, NIR-determined water correlated with short-term modulus and proteoglycan content (R = -0.40 and -0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error.
29756993	1	0	theme	specific	169:176	arg1	enzyme					178:183	a specific enzyme	167:183	a specific enzyme	167:183	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	5	1	theme	plant	881:885	arg1	tissues					887:893	plant tissues	881:893	plant tissues	881:893	The spectral regions used to build the models were optimized for each sugar and were selected in such a manner that they did not overlap with strong protein and lipid bands that generally exist in plant tissues.
29756993	6	2	theme	conventional	1048:1059	arg1	method					1061:1066	a conventional method	1046:1066	a conventional method	1046:1066	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	3	3	theme	squares	503:509	arg1	analysis					529:536	partial least squares regression (PLSR) analysis	489:536	partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase	489:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	6	4	theme	sugars	963:968	arg1	composition					940:950	the composition	936:950	the composition of reduced sugars and starch in a reactor	936:992	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	6	5	theme	reduced	955:961	arg1	sugars					963:968	reduced sugars	955:968	reduced sugars	955:968	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	8	6	theme	Raman	1307:1311	arg1	reliable					1326:1333	reliable	1326:1333	reliable	1326:1333	The results suggest that the present Raman technique is reliable and useful for sugar analysis.
29756993	8	6	theme	Raman	1307:1311	arg1	technique					1313:1321	the present Raman technique	1295:1321	the present Raman technique	1295:1321	The results suggest that the present Raman technique is reliable and useful for sugar analysis.
29756993	3	7	theme	least	497:501	arg1	squares					503:509	partial least squares	489:509	partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase	489:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	4	8	theme	models	645:650	arg1	reliability					615:625	the reliability	611:625	the reliability of the prediction models built using seven test samples	611:681	We examined the reliability of the prediction models built using seven test samples.
29756993	2	9	theme	quantitative	284:295	arg1	technique					310:318	a microvolume, quantitative, and in situ technique	269:318	a microvolume, quantitative, and in situ technique	269:318	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	2	9	theme	quantitative	284:295	arg1	It					263:264	It	263:264	It	263:264	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	4	10	theme	test	670:673	arg1	samples					675:681	seven test samples	664:681	seven test samples	664:681	We examined the reliability of the prediction models built using seven test samples.
29756993	3	11	theme	maltose	444:450	arg1	analysis					432:439	quantitative analysis	419:439	quantitative analysis of maltose, glucose, and starch	419:471	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	7	12	theme	analysis	1090:1097	arg1	results					1073:1079	The results	1069:1079	The results of Raman analysis and the conventional method	1069:1125	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	7	13	with	reactions	1213:1221	arg1	enzyme					1240:1245	a different enzyme	1228:1245	a different enzyme	1228:1245	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	7	13	with	reactions	1213:1221	arg1	β-amylase					1259:1267	β-amylase	1259:1267	β-amylase	1259:1267	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	3	14	theme	saccharification	553:568	arg1	process					570:576	the saccharification process	549:576	the saccharification process caused by α-amylase	549:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	2	15	theme	plant	382:386	arg1	tissues					388:394	plant tissues	382:394	plant tissues	382:394	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	7	16	theme	good	1134:1137	arg1	agreement					1139:1147	good agreement	1134:1147	good agreement for the reaction with α-amylase	1134:1179	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	2	17	from	processes	369:377	arg1	tissues					388:394	plant tissues	382:394	plant tissues	382:394	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	8	18	theme	present	1299:1305	arg1	reliable					1326:1333	reliable	1326:1333	reliable	1326:1333	The results suggest that the present Raman technique is reliable and useful for sugar analysis.
29756993	8	18	theme	present	1299:1305	arg1	technique					1313:1321	the present Raman technique	1295:1321	the present Raman technique	1295:1321	The results suggest that the present Raman technique is reliable and useful for sugar analysis.
29756993	5	19	theme	lipid	845:849	arg1	bands					851:855	strong protein and lipid bands	826:855	bands	851:855	The spectral regions used to build the models were optimized for each sugar and were selected in such a manner that they did not overlap with strong protein and lipid bands that generally exist in plant tissues.
29756993	0	20	theme	Analysis	28:35	arg1	Techniques					37:46	Quantitative Analysis Techniques	15:46	Quantitative Analysis Techniques for Saccharification Reactions	15:77	Development of Quantitative Analysis Techniques for Saccharification Reactions Using Raman Spectroscopy.
29756993	9	21	theme	specific	1408:1415	arg1	enzyme					1417:1422	a specific enzyme	1406:1422	a specific enzyme	1406:1422	However, the prediction model built for a specific enzyme is valid only for that enzyme.
29756993	2	22	used	used	334:337	arg2	technique					310:318	a microvolume, quantitative, and in situ technique	269:318	a microvolume, quantitative, and in situ technique	269:318	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	2	22	used	used	334:337	arg2	It					263:264	It	263:264	It	263:264	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	0	23	theme	Quantitative	15:26	arg1	Techniques					37:46	Quantitative Analysis Techniques	15:46	Quantitative Analysis Techniques for Saccharification Reactions	15:77	Development of Quantitative Analysis Techniques for Saccharification Reactions Using Raman Spectroscopy.
29756993	3	24	theme	PLSR	523:526	arg1	analysis					529:536	partial least squares regression (PLSR) analysis	489:536	partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase	489:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	2	25	theme	in	302:303	arg1	technique					310:318	a microvolume, quantitative, and in situ technique	269:318	a microvolume, quantitative, and in situ technique	269:318	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	2	25	theme	in	302:303	arg1	It					263:264	It	263:264	It	263:264	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	3	26	theme	starch	466:471	arg1	analysis					432:439	quantitative analysis	419:439	quantitative analysis of maltose, glucose, and starch	419:471	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	7	27	theme	different	1230:1238	arg1	enzyme					1240:1245	a different enzyme	1228:1245	a different enzyme	1228:1245	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	7	27	theme	different	1230:1238	arg1	β-amylase					1259:1267	β-amylase	1259:1267	β-amylase	1259:1267	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	3	28	theme	partial	489:495	arg1	squares					503:509	partial least squares	489:509	partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase	489:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	6	29	from	composition	940:950	arg1	reactor					986:992	a reactor	984:992	a reactor	984:992	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	3	30	theme	glucose	453:459	arg1	analysis					432:439	quantitative analysis	419:439	quantitative analysis of maltose, glucose, and starch	419:471	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	7	31	with	agreement	1139:1147	arg1	α-amylase					1171:1179	α-amylase	1171:1179	α-amylase	1171:1179	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	1	32	theme	Raman	215:219	arg1	spectroscopy					221:232	Raman spectroscopy	215:232	Raman spectroscopy using multivariate analysis	215:260	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	0	33	theme	Techniques	37:46	arg1	Development					0:10	Development	0:10	Development of Quantitative Analysis Techniques for Saccharification Reactions	0:77	Development of Quantitative Analysis Techniques for Saccharification Reactions Using Raman Spectroscopy.
29756993	3	34	theme	quantitative	419:430	arg1	analysis					432:439	quantitative analysis	419:439	quantitative analysis of maltose, glucose, and starch	419:471	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	6	35	dep	validated	912:920	arg1	comparing					1001:1009	comparing	1001:1009	comparing the results with those obtained by a conventional method	1001:1066	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	6	35	dep	validated	912:920	arg1	monitoring					925:934	monitoring	925:934	monitoring the composition of reduced sugars and starch in a reactor	925:992	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
29756993	7	36	theme	conventional	1107:1118	arg1	method					1120:1125	the conventional method	1103:1125	the conventional method	1103:1125	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	1	37	theme	spectroscopy	221:232	arg1	basis					206:210	the basis	202:210	the basis of Raman spectroscopy using multivariate analysis	202:260	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	3	38	theme	Prediction	397:406	arg1	models					408:413	Prediction models	397:413	Prediction models for quantitative analysis of maltose, glucose, and starch	397:471	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	0	39	theme	Saccharification	52:67	arg1	Reactions					69:77	Saccharification Reactions	52:77	Saccharification Reactions	52:77	Development of Quantitative Analysis Techniques for Saccharification Reactions Using Raman Spectroscopy.
29756993	2	40	dep	in	302:303	arg1	situ					305:308	situ	305:308	situ	305:308	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	2	41	theme	microvolume	271:281	arg1	technique					310:318	a microvolume, quantitative, and in situ technique	269:318	a microvolume, quantitative, and in situ technique	269:318	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	2	41	theme	microvolume	271:281	arg1	It					263:264	It	263:264	It	263:264	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	1	42	theme	saccharification	137:152	arg1	reactions					154:162	saccharification reactions	137:162	saccharification reactions	137:162	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	5	43	theme	spectral	688:695	arg1	regions					697:703	The spectral regions	684:703	The spectral regions used to build the models	684:728	The spectral regions used to build the models were optimized for each sugar and were selected in such a manner that they did not overlap with strong protein and lipid bands that generally exist in plant tissues.
29756993	8	44	theme	sugar	1350:1354	arg1	analysis					1356:1363	sugar analysis	1350:1363	sugar analysis	1350:1363	The results suggest that the present Raman technique is reliable and useful for sugar analysis.
29756993	5	45	theme	strong	826:831	arg1	protein					833:839	strong protein and lipid bands	826:855	protein	833:839	The spectral regions used to build the models were optimized for each sugar and were selected in such a manner that they did not overlap with strong protein and lipid bands that generally exist in plant tissues.
29756993	1	46	theme	reactions	154:162	arg1	analysis					125:132	the analysis	121:132	the analysis of saccharification reactions by a specific enzyme	121:183	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	1	47	theme	multivariate	240:251	arg1	analysis					253:260	multivariate analysis	240:260	multivariate analysis	240:260	A technique for the analysis of saccharification reactions by a specific enzyme was developed on the basis of Raman spectroscopy using multivariate analysis.
29756993	0	48	theme	Raman	85:89	arg1	Spectroscopy					91:102	Raman Spectroscopy	85:102	Raman Spectroscopy	85:102	Development of Quantitative Analysis Techniques for Saccharification Reactions Using Raman Spectroscopy.
29756993	4	49	theme	prediction	634:643	arg1	models					645:650	the prediction models	630:650	the prediction models built using seven test samples	630:681	We examined the reliability of the prediction models built using seven test samples.
29756993	3	50	theme	regression	511:520	arg1	analysis					529:536	partial least squares regression (PLSR) analysis	489:536	partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase	489:596	Prediction models for quantitative analysis of maltose, glucose, and starch were built with partial least squares regression (PLSR) analysis to monitor the saccharification process caused by α-amylase.
29756993	7	51	theme	Raman	1084:1088	arg1	analysis					1090:1097	Raman analysis	1084:1097	Raman analysis	1084:1097	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	9	52	theme	prediction	1379:1388	arg1	valid					1427:1431	valid	1427:1431	valid	1427:1431	However, the prediction model built for a specific enzyme is valid only for that enzyme.
29756993	9	52	theme	prediction	1379:1388	arg1	model					1390:1394	the prediction model	1375:1394	the prediction model built for a specific enzyme	1375:1422	However, the prediction model built for a specific enzyme is valid only for that enzyme.
29756993	7	53	theme	method	1120:1125	arg1	results					1073:1079	The results	1069:1079	The results of Raman analysis and the conventional method	1069:1125	The results of Raman analysis and the conventional method showed good agreement for the reaction with α-amylase; however, it is not perfect for reactions with a different enzyme, especially β-amylase.
29756993	2	54	theme	saccharification	352:367	arg1	processes					369:377	saccharification processes	352:377	saccharification processes in plant tissues	352:394	It is a microvolume, quantitative, and in situ technique, which can be used for studying saccharification processes in plant tissues.
29756993	6	55	theme	starch	974:979	arg1	composition					940:950	the composition	936:950	the composition of reduced sugars and starch in a reactor	936:992	The models were validated by monitoring the composition of reduced sugars and starch in a reactor and by comparing the results with those obtained by a conventional method.
31236618	4	0	theme	β-GlcNAc	1015:1022	arg1	residue					1024:1030	the β-GlcNAc residue	1011:1030	the β-GlcNAc residue	1011:1030	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	1	1	theme	significant	415:425	arg1	attention					427:435	significant attention	415:435	significant attention	415:435	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	1	1	theme	significant	415:425	arg1	alternative					443:453	an alternative	440:453	an alternative to antibiotic treatment	440:477	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	7	2	theme	novel	1442:1446	arg1	strategy					1448:1455	a novel strategy	1440:1455	a novel strategy of S. aureus which protects the bacteria from infection of SP phages	1440:1524	Finally, we describe a novel strategy of S. aureus which protects the bacteria from infection of SP phages.
31236618	4	3	theme	reading	1076:1082	arg1	ORF					1091:1093	ORF	1091:1093	ORF	1091:1093	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	4	3	theme	reading	1076:1082	arg1	frame					1084:1088	open reading frame	1071:1088	open reading frame (ORF) 100 and ORF102	1071:1109	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	8	4	theme	TarM	1585:1588	arg1	TarS					1597:1600	TarM or/and TarS	1585:1600	TarM or/and TarS	1585:1600	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	3	5	theme	teichoic	793:800	arg1	WTA					808:810	WTA	808:810	WTA	808:810	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	3	5	theme	teichoic	793:800	arg1	acid					802:805	wall teichoic acid	788:805	wall teichoic acid (WTA)	788:811	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	2	6	theme	host	568:571	arg1	range					573:577	broad host range	562:577	broad host range	562:577	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	8	7	theme	SP	1637:1638	arg1	phages					1640:1645	SP phages	1637:1645	SP phages	1637:1645	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	3	8	theme	β-GlcNAc	884:891	arg1	residue					894:900	the β-N-acetylglucosamine (β-GlcNAc) residue	857:900	the β-N-acetylglucosamine (β-GlcNAc) residue in WTA	857:907	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	9	9	theme	interactions	1836:1847	arg1	biology					1814:1820	the biology	1810:1820	the biology of phage-host interactions	1810:1847	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	8	10	theme	or/and	1590:1595	arg1	TarS					1597:1600	TarM or/and TarS	1585:1600	TarM or/and TarS	1585:1600	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	6	11	theme	pseudintermedius	1344:1359	arg1	glycosylation					1317:1329	glycosylation	1317:1329	glycosylation in WTA of S. pseudintermedius	1317:1359	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	12	theme	pseudintermedius-specific	1253:1277	arg1	phage					1279:1283	newly isolated S. pseudintermedius-specific phage	1235:1283	newly isolated S. pseudintermedius-specific phage (SP phages)	1235:1295	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	12	theme	pseudintermedius-specific	1253:1277	arg1	phages					1289:1294	SP phages	1286:1294	SP phages	1286:1294	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	8	13	theme	aureus	1627:1632	arg1	infection					1611:1619	infection	1611:1619	infection of S. aureus	1611:1632	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	3	14	from	those	716:720	arg1	distinct					702:709	distinct	702:709	distinct	702:709	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	0	15	theme	ɸSA039	63:68	arg1	mechanism					26:34	Analysis host-recognition mechanism	0:34	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039	0:68	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	3	16	theme	acid	802:805	arg1	backbone					776:783	the backbone	772:783	the backbone of wall teichoic acid (WTA)	772:811	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	2	17	dep	known	529:533	arg1	previously					518:527	previously	518:527	previously	518:527	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	0	18	theme	Analysis	0:7	arg1	mechanism					26:34	Analysis host-recognition mechanism	0:34	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039	0:68	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	8	19	theme	phosphate	1561:1569	arg1	WTA					1578:1580	ribitol phosphate (RboP) WTA	1553:1580	ribitol phosphate (RboP) WTA	1553:1580	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	0	20	theme	host-recognition	9:24	arg1	mechanism					26:34	Analysis host-recognition mechanism	0:34	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039	0:68	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	1	21	theme	antibiotic	458:467	arg1	treatment					469:477	antibiotic treatment	458:477	antibiotic treatment	458:477	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	4	22	from	mutation	1059:1066	arg1	ORF					1091:1093	ORF	1091:1093	ORF	1091:1093	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	4	22	from	mutation	1059:1066	arg1	frame					1084:1088	open reading frame	1071:1088	open reading frame (ORF) 100 and ORF102	1071:1109	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	2	23	contain	have	557:560	arg1	Bacteriophages					480:493	Bacteriophages	480:493	Bacteriophages belonging to kayvirus (previously known as Twort-like phages)	480:555	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	2	23	contain	have	557:560	arg2	range					573:577	broad host range	562:577	broad host range	562:577	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	8	24	gly	glycosylation	1536:1548	arg1	WTA					1578:1580	ribitol phosphate (RboP) WTA	1553:1580	ribitol phosphate (RboP) WTA	1553:1580	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	5	25	contain	has	1169:1171	arg2	structure					1189:1197	a different WTA structure	1173:1197	a different WTA structure	1173:1197	Moreover, ɸSA039 could infect S. pseudintermedius, which has a different WTA structure than S. aureus.
31236618	5	25	contain	has	1169:1171	arg1	pseudintermedius					1145:1160	S. pseudintermedius	1142:1160	S. pseudintermedius	1142:1160	Moreover, ɸSA039 could infect S. pseudintermedius, which has a different WTA structure than S. aureus.
31236618	2	26	theme	Twort-like	538:547	arg1	phages					549:554	Twort-like phages	538:554	Twort-like phages	538:554	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	5	27	theme	different	1175:1183	arg1	structure					1189:1197	a different WTA structure	1173:1197	a different WTA structure	1173:1197	Moreover, ɸSA039 could infect S. pseudintermedius, which has a different WTA structure than S. aureus.
31236618	6	28	theme	isolated	1241:1248	arg1	phage					1279:1283	newly isolated S. pseudintermedius-specific phage	1235:1283	newly isolated S. pseudintermedius-specific phage (SP phages)	1235:1295	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	28	theme	isolated	1241:1248	arg1	phages					1289:1294	SP phages	1286:1294	SP phages	1286:1294	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	7	29	theme	aureus	1463:1468	arg1	strategy					1448:1455	a novel strategy	1440:1455	a novel strategy of S. aureus which protects the bacteria from infection of SP phages	1440:1524	Finally, we describe a novel strategy of S. aureus which protects the bacteria from infection of SP phages.
31236618	4	30	theme	spontaneous	1047:1057	arg1	mutation					1059:1066	a spontaneous mutation	1045:1066	a spontaneous mutation in open reading frame (ORF) 100 and ORF102	1045:1109	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	1	31	theme	antibiotic-resistant	232:251	arg1	pseudintermedius					362:377	Staphylococcus pseudintermedius	347:377	methicillin-resistant Staphylococcus pseudintermedius (MRSP)	325:384	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	1	31	theme	antibiotic-resistant	232:251	arg1	aureus					307:312	Staphylococcus aureus	292:312	methicillin-resistant Staphylococcus aureus (MRSA)	270:319	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	1	31	theme	antibiotic-resistant	232:251	arg1	bacteria					253:260	antibiotic-resistant bacteria	232:260	antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP)	232:384	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	4	32	theme	open	1071:1074	arg1	ORF					1091:1093	ORF	1091:1093	ORF	1091:1093	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	4	32	theme	open	1071:1074	arg1	frame					1084:1088	open reading frame	1071:1088	open reading frame (ORF) 100 and ORF102	1071:1109	In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102.
31236618	5	33	theme	WTA	1185:1187	arg1	structure					1189:1197	a different WTA structure	1173:1197	a different WTA structure	1173:1197	Moreover, ɸSA039 could infect S. pseudintermedius, which has a different WTA structure than S. aureus.
31236618	9	34	theme	pseudintermedius	1740:1755	arg1	infection					1757:1765	S. aureus and S. pseudintermedius infection	1723:1765	S. aureus and S. pseudintermedius infection	1723:1765	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	1	35	theme	bacteria	253:260	arg1	emergence					219:227	the emergence	215:227	the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP)	215:384	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	9	36	theme	infection	1757:1765	arg1	treatment					1710:1718	the treatment	1706:1718	the treatment of S. aureus and S. pseudintermedius infection	1706:1765	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	3	37	contain	has	669:671	arg1	kayvirus					653:660	kayvirus ɸSA039	653:667	kayvirus ɸSA039	653:667	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	3	37	contain	has	669:671	arg2	mechanism					692:700	a host-recognition mechanism	673:700	a host-recognition mechanism distinct from those of other known kayviruses	673:746	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	0	38	theme	kayvirus	54:61	arg1	ɸSA039					63:68	staphylococcal kayvirus ɸSA039	39:68	staphylococcal kayvirus ɸSA039	39:68	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	6	39	theme	SP	1392:1393	arg1	phages					1395:1400	SP phages	1392:1400	SP phages	1392:1400	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	0	40	theme	pseudintermedius	167:182	arg1	phages					197:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	7	41	theme	SP	1516:1517	arg1	phages					1519:1524	SP phages	1516:1524	SP phages	1516:1524	Finally, we describe a novel strategy of S. aureus which protects the bacteria from infection of SP phages.
31236618	2	42	dep	kayvirus	508:515	arg1	known					529:533	known	529:533	known as Twort-like phages	529:554	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	6	43	gly	glycosylation	1317:1329	arg1	phages					1395:1400	SP phages	1392:1400	SP phages	1392:1400	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	43	gly	glycosylation	1317:1329	arg1	WTA					1334:1336	WTA	1334:1336	WTA	1334:1336	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	43	gly	glycosylation	1317:1329	arg1	pseudintermedius					1344:1359	S. pseudintermedius	1341:1359	S. pseudintermedius	1341:1359	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	2	44	theme	broad	562:566	arg1	range					573:577	broad host range	562:577	broad host range	562:577	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	0	45	theme	staphylococcal	39:52	arg1	ɸSA039					63:68	staphylococcal kayvirus ɸSA039	39:68	staphylococcal kayvirus ɸSA039	39:68	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	3	46	theme	known	731:735	arg1	kayviruses					737:746	other known kayviruses	725:746	other known kayviruses	725:746	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	3	47	theme	distinct	702:709	arg1	mechanism					692:700	a host-recognition mechanism	673:700	a host-recognition mechanism distinct from those of other known kayviruses	673:746	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	9	48	theme	aureus	1726:1731	arg1	infection					1757:1765	S. aureus and S. pseudintermedius infection	1723:1765	S. aureus and S. pseudintermedius infection	1723:1765	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	1	49	theme	phage	387:391	arg1	therapy					393:399	phage therapy	387:399	phage therapy	387:399	Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment.
31236618	6	50	from	glycosylation	1317:1329	arg1	WTA					1334:1336	WTA	1334:1336	WTA	1334:1336	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	7	51	theme	phages	1519:1524	arg1	infection					1503:1511	infection	1503:1511	infection of SP phages	1503:1524	Finally, we describe a novel strategy of S. aureus which protects the bacteria from infection of SP phages.
31236618	0	52	theme	Staphylococcus	152:165	arg1	phages					197:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	3	53	from	residue	894:900	arg1	WTA					905:907	WTA	905:907	WTA	905:907	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	0	54	theme	Siphoviridae	184:195	arg1	phages					197:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Staphylococcus pseudintermedius Siphoviridae phages	152:202	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	3	55	theme	host-recognition	675:690	arg1	mechanism					692:700	a host-recognition mechanism	673:700	a host-recognition mechanism distinct from those of other known kayviruses	673:746	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	6	56	theme	SP	1286:1287	arg1	phage					1279:1283	newly isolated S. pseudintermedius-specific phage	1235:1283	newly isolated S. pseudintermedius-specific phage (SP phages)	1235:1295	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	6	56	theme	SP	1286:1287	arg1	phages					1289:1294	SP phages	1286:1294	SP phages	1286:1294	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	8	57	theme	ribitol	1553:1559	arg1	WTA					1578:1580	ribitol phosphate (RboP) WTA	1553:1580	ribitol phosphate (RboP) WTA	1553:1580	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	9	58	theme	new	1689:1691	arg1	strategy					1693:1700	a new strategy	1687:1700	a new strategy for the treatment of S. aureus and S. pseudintermedius infection	1687:1765	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	2	59	from	lytic	596:600	arg1	spp					620:622	Staphylococcus spp	605:622	Staphylococcus spp	605:622	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	9	60	theme	valuable	1787:1794	arg1	insights					1796:1803	valuable insights	1787:1803	valuable insights into the biology of phage-host interactions	1787:1847	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	3	61	theme	β-N-acetylglucosamine	861:881	arg1	residue					894:900	the β-N-acetylglucosamine (β-GlcNAc) residue	857:900	the β-N-acetylglucosamine (β-GlcNAc) residue in WTA	857:907	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	3	62	theme	Previous	625:632	arg1	work					634:637	Previous work	625:637	Previous work	625:637	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	2	63	from	spp	620:622	arg1	lytic					596:600	lytic	596:600	lytic	596:600	Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp.
31236618	3	64	theme	other	725:729	arg1	kayviruses					737:746	other known kayviruses	725:746	other known kayviruses	725:746	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	0	65	theme	novel	80:84	arg1	strategy					86:93	a novel strategy	78:93	a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages	78:202	Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
31236618	9	66	theme	phage-host	1825:1834	arg1	interactions					1836:1847	phage-host interactions	1825:1847	phage-host interactions	1825:1847	These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
31236618	6	67	theme	phages	1395:1400	arg1	adsorption					1378:1387	adsorption	1378:1387	adsorption of SP phages	1378:1400	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	8	68	theme	WTA	1578:1580	arg1	glycosylation					1536:1548	glycosylation	1536:1548	glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS	1536:1600	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
31236618	6	69	dep	isolated	1241:1248	arg1	S.					1250:1251	S.	1250:1251	S.	1250:1251	By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039.
31236618	3	70	theme	wall	788:791	arg1	WTA					808:810	WTA	808:810	WTA	808:810	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	3	70	theme	wall	788:791	arg1	acid					802:805	wall teichoic acid	788:805	wall teichoic acid (WTA)	788:811	Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA.
31236618	8	71	theme	RboP	1572:1575	arg1	WTA					1578:1580	ribitol phosphate (RboP) WTA	1553:1580	ribitol phosphate (RboP) WTA	1553:1580	Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages.
30839970	8	0	theme	scavenging	1140:1149	arg1	properties					1151:1160	strong scavenging properties	1133:1160	strong scavenging properties	1133:1160	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	8	1	from	radical	1215:1221	arg1	ion					1276:1278	ferrous ion	1268:1278	ferrous ion	1268:1278	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	11	2	theme	d-gal-induced	1684:1696	arg1	mice					1698:1701	d-gal-induced mice	1684:1701	d-gal-induced mice	1684:1701	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	11	3	theme	microbiota	1658:1667	arg1	composition					1669:1679	the gut microbiota composition	1650:1679	the gut microbiota composition in d-gal-induced mice	1650:1701	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	11	4	theme	rank	1559:1562	arg1	correlation					1564:1574	Spearman's rank correlation	1548:1574	Spearman's rank correlation	1548:1574	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	3	5	theme	high	509:512	arg1	capacity					526:533	high antioxidant capacity	509:533	high antioxidant capacity	509:533	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	12	6	theme	gut	1814:1816	arg1	composition					1829:1839	the gut microbiota composition	1810:1839	the gut microbiota composition	1810:1839	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	8	7	theme	superoxide	1204:1213	arg1	radical					1215:1221	superoxide radical	1204:1221	superoxide radical	1204:1221	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	6	8	theme	in	879:880	arg1	methods					888:894	in vitro methods	879:894	in vitro methods	879:894	Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods.
30839970	8	9	theme	ferrous	1268:1274	arg1	ion					1276:1278	ferrous ion	1268:1278	ferrous ion	1268:1278	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	1	10	theme	degenerative	194:205	arg1	diseases					207:214	degenerative diseases	194:214	degenerative diseases	194:214	Correlations between oxidative stress and degenerative diseases have been gaining increasing attention.
30839970	0	11	theme	alleviative	112:122	arg1	effect					124:129	the alleviative effect	108:129	the alleviative effect on oxidative stress	108:149	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	7	12	theme	gut	1039:1041	arg1	disorder					1054:1061	gut microbiota disorder	1039:1061	gut microbiota disorder	1039:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	1	13	theme	gaining	226:232	arg1	attention					245:253	gaining increasing attention	226:253	gaining increasing attention	226:253	Correlations between oxidative stress and degenerative diseases have been gaining increasing attention.
30839970	8	14	from	activity	1256:1263	arg1	ion					1276:1278	ferrous ion	1268:1278	ferrous ion	1268:1278	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	7	15	theme	-induced	1013:1020	arg1	damage					1028:1033	d-galactose (d-gal)-induced liver damage	994:1033	d-galactose (d-gal)-induced liver damage	994:1033	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	2	16	theme	studies	268:274	arg1	number					258:263	A number	256:263	A number of studies	256:274	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	12	17	theme	oxidative	1776:1784	arg1	stress					1786:1791	hepatic oxidative stress	1768:1791	hepatic oxidative stress	1768:1791	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	0	18	theme	exopolysaccharide	38:54	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of an exopolysaccharide	0:54	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	7	19	theme	protective	965:974	arg1	effects					976:982	the possible protective effects	952:982	the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder	952:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	3	20	contain	possess	501:507	arg2	capacity					526:533	high antioxidant capacity	509:533	high antioxidant capacity	509:533	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	3	20	contain	possess	501:507	arg1	helveticus					461:470	Lactobacillus helveticus KLDS1.8701	447:481	Lactobacillus helveticus KLDS1.8701	447:481	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	10	21	theme	microbiota	1466:1475	arg1	composition					1477:1487	the gut microbiota composition	1458:1487	the gut microbiota composition	1458:1487	EPS-1 supplementation shifted the gut microbiota composition to that of the control group.
30839970	10	22	theme	control	1504:1510	arg1	group					1512:1516	the control group	1500:1516	the control group	1500:1516	EPS-1 supplementation shifted the gut microbiota composition to that of the control group.
30839970	3	23	theme	previous	431:438	arg1	study					440:444	our previous study	427:444	our previous study	427:444	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	5	24	theme	helveticus	722:731	arg1	culture					708:714	the culture	704:714	the culture of L. helveticus KLDS1.8701	704:742	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	0	25	theme	In	0:1	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of an exopolysaccharide	0:54	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	9	26	theme	organic	1372:1378	arg1	index					1380:1384	decreased organic index	1362:1384	decreased organic index	1362:1384	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	9	27	dep	index	1380:1384	arg1	stress					1420:1425	oxidative stress	1410:1425	oxidative stress	1410:1425	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	2	28	theme	related	406:412	arg1	diseases					414:421	the related diseases	402:421	the related diseases	402:421	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	5	29	theme	Fast	778:781	arg1	chromatography					788:801	DEAE-Sepharose Fast Flow chromatography	763:801	DEAE-Sepharose Fast Flow chromatography	763:801	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	8	30	theme	activity	1085:1092	arg1	results					1094:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	8	31	from	radical	1195:1201	arg1	ion					1276:1278	ferrous ion	1268:1278	ferrous ion	1268:1278	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	7	32	theme	in	909:910	arg1	study					917:921	an in vivo study	906:921	an in vivo study	906:921	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	1	33	theme	increasing	234:243	arg1	attention					245:253	gaining increasing attention	226:253	gaining increasing attention	226:253	Correlations between oxidative stress and degenerative diseases have been gaining increasing attention.
30839970	6	34	dep	in	879:880	arg1	vitro					882:886	vitro	882:886	vitro	882:886	Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods.
30839970	11	35	theme	gut	1654:1656	arg1	composition					1669:1679	the gut microbiota composition	1650:1679	the gut microbiota composition in d-gal-induced mice	1650:1701	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	2	36	theme	oxidative	365:373	arg1	stress					375:380	oxidative stress	365:380	oxidative stress	365:380	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	8	37	theme	chelating	1246:1254	arg1	activity					1256:1263	chelating activity	1246:1263	chelating activity	1246:1263	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	0	38	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	4	39	from	effects	600:606	arg1	stress					665:670	oxidative stress	655:670	oxidative stress	655:670	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30839970	8	40	theme	strong	1133:1138	arg1	properties					1151:1160	strong scavenging properties	1133:1160	strong scavenging properties	1133:1160	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	12	41	theme	microbiota	1818:1827	arg1	composition					1829:1839	the gut microbiota composition	1810:1839	the gut microbiota composition	1810:1839	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	11	42	theme	correlation	1564:1574	arg1	analysis					1536:1543	the analysis	1532:1543	the analysis of Spearman's rank correlation	1532:1574	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	12	43	used	used	1848:1851	arg2	candidate					1868:1876	a potential candidate	1856:1876	a potential candidate to attenuate oxidative damage	1856:1906	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	12	43	used	used	1848:1851	arg2	supplementation					1737:1751	EPS-1 supplementation	1731:1751	EPS-1 supplementation	1731:1751	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	7	44	dep	in	909:910	arg1	vivo					912:915	vivo	912:915	vivo	912:915	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	1	45	theme	oxidative	173:181	arg1	stress					183:188	oxidative stress	173:188	oxidative stress	173:188	Correlations between oxidative stress and degenerative diseases have been gaining increasing attention.
30839970	8	46	theme	2,2-diphenyl-1-picrylhydrazyl	1165:1193	arg1	radical					1195:1201	2,2-diphenyl-1-picrylhydrazyl radical	1165:1201	2,2-diphenyl-1-picrylhydrazyl radical	1165:1201	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	8	47	from	radical	1233:1239	arg1	ion					1276:1278	ferrous ion	1268:1278	ferrous ion	1268:1278	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	7	48	from	effects	976:982	arg1	damage					1028:1033	d-galactose (d-gal)-induced liver damage	994:1033	d-galactose (d-gal)-induced liver damage	994:1033	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	7	48	from	effects	976:982	arg1	disorder					1054:1061	gut microbiota disorder	1039:1061	gut microbiota disorder	1039:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	11	49	theme	protective	1595:1604	arg1	effects					1606:1612	the protective effects	1591:1612	the protective effects of EPS	1591:1619	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	2	50	theme	lactic	324:329	arg1	LAB					346:348	LAB	346:348	LAB	346:348	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	2	50	theme	lactic	324:329	arg1	bacteria					336:343	lactic acid bacteria	324:343	lactic acid bacteria (LAB)	324:349	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	12	51	theme	oxidative	1891:1899	arg1	damage					1901:1906	oxidative damage	1891:1906	oxidative damage	1891:1906	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	6	52	theme	EPS	844:846	arg1	fractions					848:856	EPS fractions	844:856	EPS fractions	844:856	Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods.
30839970	4	53	theme	oxidative	655:663	arg1	stress					665:670	oxidative stress	655:670	oxidative stress	655:670	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30839970	7	54	theme	microbiota	1043:1052	arg1	disorder					1054:1061	gut microbiota disorder	1039:1061	gut microbiota disorder	1039:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	10	55	theme	EPS-1	1428:1432	arg1	supplementation					1434:1448	EPS-1 supplementation	1428:1448	EPS-1 supplementation	1428:1448	EPS-1 supplementation shifted the gut microbiota composition to that of the control group.
30839970	0	56	theme	oxidative	134:142	arg1	stress					144:149	oxidative stress	134:149	oxidative stress	134:149	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	8	57	theme	hydroxyl	1224:1231	arg1	radical					1233:1239	hydroxyl radical	1224:1239	hydroxyl radical	1224:1239	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	4	58	theme	L.	627:628	arg1	helveticus					630:639	L. helveticus KLDS1.8701	627:650	L. helveticus KLDS1.8701	627:650	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30839970	8	59	dep	In	1064:1065	arg1	vitro					1067:1071	vitro	1067:1071	vitro	1067:1071	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	7	60	theme	liver	1022:1026	arg1	damage					1028:1033	d-galactose (d-gal)-induced liver damage	994:1033	d-galactose (d-gal)-induced liver damage	994:1033	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	7	61	theme	EPS	987:989	arg1	effects					976:982	the possible protective effects	952:982	the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder	952:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	12	62	theme	hepatic	1768:1774	arg1	stress					1786:1791	hepatic oxidative stress	1768:1791	hepatic oxidative stress	1768:1791	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	0	63	theme	Lactobacillus	68:80	arg1	helveticus					82:91	Lactobacillus helveticus KLDS1.8701	68:102	Lactobacillus helveticus KLDS1.8701	68:102	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	9	64	theme	EPS-1	1290:1294	arg1	supplementation					1296:1310	EPS-1 supplementation	1290:1310	EPS-1 supplementation	1290:1310	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	7	65	theme	possible	956:963	arg1	effects					976:982	the possible protective effects	952:982	the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder	952:1061	Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder.
30839970	12	66	theme	EPS-1	1731:1735	arg1	candidate					1868:1876	a potential candidate	1856:1876	a potential candidate to attenuate oxidative damage	1856:1906	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	12	66	theme	EPS-1	1731:1735	arg1	supplementation					1737:1751	EPS-1 supplementation	1731:1751	EPS-1 supplementation	1731:1751	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	10	67	theme	gut	1462:1464	arg1	composition					1477:1487	the gut microbiota composition	1458:1487	the gut microbiota composition	1458:1487	EPS-1 supplementation shifted the gut microbiota composition to that of the control group.
30839970	5	68	attach	isolated	690:697	arg1	culture					708:714	the culture	704:714	the culture of L. helveticus KLDS1.8701	704:742	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	5	68	attach	isolated	690:697	arg2	EPS					682:684	EPS	682:684	EPS	682:684	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	5	69	theme	L.	719:720	arg1	helveticus					722:731	L. helveticus KLDS1.8701	719:742	L. helveticus KLDS1.8701	719:742	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	9	70	theme	oxidative	1337:1345	arg1	status					1347:1352	oxidative status	1337:1352	oxidative status such as decreased organic index, liver injury and liver oxidative stress	1337:1425	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	4	71	theme	ameliorative	587:598	arg1	effects					600:606	the ameliorative effects	583:606	the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress	583:670	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30839970	0	72	theme	in	13:14	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of an exopolysaccharide	0:54	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	9	73	theme	decreased	1362:1370	arg1	index					1380:1384	decreased organic index	1362:1384	decreased organic index	1362:1384	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	8	74	theme	In	1064:1065	arg1	results					1094:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	4	75	theme	study	561:565	arg1	aim					549:551	The aim	545:551	The aim of this study	545:565	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30839970	3	76	theme	antioxidant	514:524	arg1	capacity					526:533	high antioxidant capacity	509:533	high antioxidant capacity	509:533	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	5	77	theme	DEAE-Sepharose	763:776	arg1	chromatography					788:801	DEAE-Sepharose Fast Flow chromatography	763:801	DEAE-Sepharose Fast Flow chromatography	763:801	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	8	78	theme	antioxidant	1073:1083	arg1	results					1094:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results	1064:1100	In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion.
30839970	11	79	from	composition	1669:1679	arg1	mice					1698:1701	d-gal-induced mice	1684:1701	d-gal-induced mice	1684:1701	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	0	80	from	effect	124:129	arg1	stress					144:149	oxidative stress	134:149	oxidative stress	134:149	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	11	81	theme	EPS	1617:1619	arg1	effects					1606:1612	the protective effects	1591:1612	the protective effects of EPS	1591:1619	In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice.
30839970	3	82	theme	Lactobacillus	447:459	arg1	helveticus					461:470	Lactobacillus helveticus KLDS1.8701	447:481	Lactobacillus helveticus KLDS1.8701	447:481	In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro.
30839970	5	83	theme	Flow	783:786	arg1	chromatography					788:801	DEAE-Sepharose Fast Flow chromatography	763:801	DEAE-Sepharose Fast Flow chromatography	763:801	Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography.
30839970	9	84	theme	liver	1387:1391	arg1	injury					1393:1398	liver injury	1387:1398	liver injury	1387:1398	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	6	85	theme	fractions	848:856	arg1	capacities					830:839	the antioxidant capacities	814:839	the antioxidant capacities of EPS fractions	814:856	Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods.
30839970	12	86	theme	potential	1858:1866	arg1	candidate					1868:1876	a potential candidate	1856:1876	a potential candidate to attenuate oxidative damage	1856:1906	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	12	86	theme	potential	1858:1866	arg1	supplementation					1737:1751	EPS-1 supplementation	1731:1751	EPS-1 supplementation	1731:1751	These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
30839970	0	87	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress.
30839970	2	88	theme	acid	331:334	arg1	LAB					346:348	LAB	346:348	LAB	346:348	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	2	88	theme	acid	331:334	arg1	bacteria					336:343	lactic acid bacteria	324:343	lactic acid bacteria (LAB)	324:349	A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases.
30839970	9	89	theme	oxidative	1410:1418	arg1	stress					1420:1425	oxidative stress	1410:1425	oxidative stress	1410:1425	In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress.
30839970	6	90	theme	antioxidant	818:828	arg1	capacities					830:839	the antioxidant capacities	814:839	the antioxidant capacities of EPS fractions	814:856	Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods.
30839970	4	91	theme	EPS	611:613	arg1	effects					600:606	the ameliorative effects	583:606	the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress	583:670	The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress.
30393138	0	0	theme	chitosan	80:87	arg1	Composite					0:8	Composite	0:8	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	0	theme	chitosan	80:87	arg1	chitosan					80:87	chitosan	80:87	chitosan	80:87	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	0	theme	chitosan	80:87	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	0	theme	chitosan	80:87	arg1	oxide					70:74	graphene oxide	61:74	graphene oxide	61:74	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	3	1	theme	magnetic	647:654	arg1	nanoparticles					656:668	magnetic nanoparticles	647:668	magnetic nanoparticles	647:668	Moreover, IL itself could exert catalytic activity and improve anchoring Pd, while magnetic nanoparticles could render the catalyst easily separable.
30393138	7	2	theme	recyclability	1085:1097	arg1	investigation					1055:1067	The investigation	1051:1067	The investigation of the catalyst recyclability	1051:1097	The investigation of the catalyst recyclability confirmed that the catalyst could be recycled for three reaction runs.
30393138	4	3	from	groups	739:744	arg1	CS					749:750	CS	749:750	CS	749:750	Moreover, the functional groups on CS could contribute to Pd anchoring.
30393138	1	4	theme	liquid	203:208	arg1	CDNS-IL					245:251	CDNS-IL	245:251	CDNS-IL	245:251	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	4	theme	liquid	203:208	arg1	nanosponge					233:242	ionic liquid decorated cyclodextrin nanosponge	197:242	ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	197:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	2	5	theme	transfer	548:555	arg1	agent					557:561	phase transfer agent	542:561	phase transfer agent	542:561	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	5	6	from	hydrogenation	842:854	arg1	media					910:914	aqueous media	902:914	aqueous media	902:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	1	7	theme	decorated	210:218	arg1	CDNS-IL					245:251	CDNS-IL	245:251	CDNS-IL	245:251	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	7	theme	decorated	210:218	arg1	nanosponge					233:242	ionic liquid decorated cyclodextrin nanosponge	197:242	ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	197:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	8	theme	Pd	307:308	arg1	nanoparticles					320:332	Pd and Fe3O4 nanoparticles	307:332	Pd and Fe3O4 nanoparticles	307:332	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	2	9	theme	phase	542:546	arg1	agent					557:561	phase transfer agent	542:561	phase transfer agent	542:561	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	3	10	theme	anchoring	627:635	arg1	Pd					637:638	anchoring Pd	627:638	anchoring Pd	627:638	Moreover, IL itself could exert catalytic activity and improve anchoring Pd, while magnetic nanoparticles could render the catalyst easily separable.
30393138	1	11	theme	cyclodextrin	220:231	arg1	CDNS-IL					245:251	CDNS-IL	245:251	CDNS-IL	245:251	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	11	theme	cyclodextrin	220:231	arg1	nanosponge					233:242	ionic liquid decorated cyclodextrin nanosponge	197:242	ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	197:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	2	12	theme	CDNS	472:475	arg1	capability					458:467	the capability	454:467	the capability of CDNS for formation of inclusion complex with substrates	454:526	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	7	13	theme	catalyst	1076:1083	arg1	recyclability					1085:1097	the catalyst recyclability	1072:1097	the catalyst recyclability	1072:1097	The investigation of the catalyst recyclability confirmed that the catalyst could be recycled for three reaction runs.
30393138	0	14	theme	catalyst	98:105	arg1	support					107:113	A novel catalyst support	90:113	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	1	15	theme	Fe3O4	314:318	arg1	nanoparticles					320:332	Pd and Fe3O4 nanoparticles	307:332	Pd and Fe3O4 nanoparticles	307:332	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	4	16	theme	functional	728:737	arg1	groups					739:744	the functional groups	724:744	the functional groups on CS	724:750	Moreover, the functional groups on CS could contribute to Pd anchoring.
30393138	6	17	theme	mild	1026:1029	arg1	condition					1040:1048	mild reaction condition	1026:1048	mild reaction condition	1026:1048	The results established that the catalyst could efficiently promote all the reactions under mild reaction condition.
30393138	1	18	theme	graphene	158:165	arg1	oxide					167:171	graphene oxide	158:171	graphene oxide (GO)	158:176	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	0	19	theme	novel	92:96	arg1	support					107:113	A novel catalyst support	90:113	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	1	20	theme	nanoparticles	320:332	arg1	immobilization					289:302	the immobilization	285:302	the immobilization of Pd and Fe3O4 nanoparticles	285:332	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	5	21	theme	Fe3O4/Pd	812:819	arg1	Hybrid					821:826	Fe3O4/Pd@Hybrid	812:826	Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media	812:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	5	22	theme	coupling	880:887	arg1	reactions					889:897	two coupling reactions	876:897	two coupling reactions in aqueous media	876:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	5	23	theme	@	820:820	arg1	Hybrid					821:826	Fe3O4/Pd@Hybrid	812:826	Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media	812:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	3	24	theme	separable	703:711	arg1	catalyst					687:694	the catalyst	683:694	the catalyst easily separable	683:711	Moreover, IL itself could exert catalytic activity and improve anchoring Pd, while magnetic nanoparticles could render the catalyst easily separable.
30393138	5	25	theme	Hybrid	821:826	arg1	activity					800:807	The catalytic activity	786:807	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media	786:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	0	26	theme	liquid	19:24	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	6	27	theme	reaction	1031:1038	arg1	condition					1040:1048	mild reaction condition	1026:1048	mild reaction condition	1026:1048	The results established that the catalyst could efficiently promote all the reactions under mild reaction condition.
30393138	2	28	theme	complex	504:510	arg1	formation					481:489	formation	481:489	formation of inclusion complex with substrates	481:526	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	4	29	theme	Pd	772:773	arg1	anchoring					775:783	Pd anchoring	772:783	Pd anchoring	772:783	Moreover, the functional groups on CS could contribute to Pd anchoring.
30393138	0	30	theme	ionic	13:17	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	2	31	theme	inclusion	494:502	arg1	complex					504:510	inclusion complex	494:510	inclusion complex with substrates	494:526	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	5	32	theme	aqueous	902:908	arg1	media					910:914	aqueous media	902:914	aqueous media	902:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	0	33	theme	cyclodextrin	36:47	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	3	34	theme	catalytic	596:604	arg1	activity					606:613	catalytic activity	596:613	catalytic activity	596:613	Moreover, IL itself could exert catalytic activity and improve anchoring Pd, while magnetic nanoparticles could render the catalyst easily separable.
30393138	5	35	theme	catalytic	790:798	arg1	activity					800:807	The catalytic activity	786:807	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media	786:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	0	36	theme	decorated	26:34	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	2	37	theme	hybrid	420:425	arg1	system					427:432	this hybrid system	415:432	this hybrid system	415:432	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	1	38	theme	magnetic	347:354	arg1	catalyst					356:363	a magnetic catalyst	345:363	a magnetic catalyst	345:363	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	38	theme	magnetic	347:354	arg1	Hybrid					375:380	Fe3O4/Pd@Hybrid	366:380	Fe3O4/Pd@Hybrid	366:380	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	5	39	theme	nitro	859:863	arg1	arenes					865:870	nitro arenes	859:870	nitro arenes	859:870	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	0	40	theme	nanosponge	49:58	arg1	Composite					0:8	Composite	0:8	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	40	theme	nanosponge	49:58	arg1	chitosan					80:87	chitosan	80:87	chitosan	80:87	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	40	theme	nanosponge	49:58	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	40	theme	nanosponge	49:58	arg1	oxide					70:74	graphene oxide	61:74	graphene oxide	61:74	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	1	41	dep	oxide	167:171	arg1	GO					174:175	GO	174:175	GO	174:175	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	5	42	theme	arenes	865:870	arg1	hydrogenation					842:854	hydrogenation	842:854	hydrogenation of nitro arenes	842:870	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	5	42	theme	arenes	865:870	arg1	reactions					889:897	two coupling reactions	876:897	two coupling reactions in aqueous media	876:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	1	43	theme	Fe3O4/Pd	366:373	arg1	catalyst					356:363	a magnetic catalyst	345:363	a magnetic catalyst	345:363	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	43	theme	Fe3O4/Pd	366:373	arg1	Hybrid					375:380	Fe3O4/Pd@Hybrid	366:380	Fe3O4/Pd@Hybrid	366:380	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	44	theme	@	374:374	arg1	catalyst					356:363	a magnetic catalyst	345:363	a magnetic catalyst	345:363	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	44	theme	@	374:374	arg1	Hybrid					375:380	Fe3O4/Pd@Hybrid	366:380	Fe3O4/Pd@Hybrid	366:380	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	45	theme	biocompatible	118:130	arg1	system					139:144	A biocompatible hybrid system	116:144	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	116:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	2	46	with	complex	504:510	arg1	substrates					517:526	substrates	517:526	substrates	517:526	The justification for designing this hybrid system was benefiting from the capability of CDNS for formation of inclusion complex with substrates and acting as phase transfer agent.
30393138	0	47	theme	oxide	70:74	arg1	Composite					0:8	Composite	0:8	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	47	theme	oxide	70:74	arg1	chitosan					80:87	chitosan	80:87	chitosan	80:87	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	47	theme	oxide	70:74	arg1	nanosponge					49:58	ionic liquid decorated cyclodextrin nanosponge	13:58	ionic liquid decorated cyclodextrin nanosponge	13:58	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	0	47	theme	oxide	70:74	arg1	oxide					70:74	graphene oxide	61:74	graphene oxide	61:74	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	1	48	theme	hybrid	132:137	arg1	system					139:144	A biocompatible hybrid system	116:144	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	116:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	5	49	from	reactions	889:897	arg1	media					910:914	aqueous media	902:914	aqueous media	902:914	The catalytic activity of Fe3O4/Pd@Hybrid for promoting hydrogenation of nitro arenes and two coupling reactions in aqueous media was investigated.
30393138	0	50	theme	graphene	61:68	arg1	oxide					70:74	graphene oxide	61:74	graphene oxide	61:74	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	7	51	theme	reaction	1155:1162	arg1	runs					1164:1167	three reaction runs	1149:1167	three reaction runs	1149:1167	The investigation of the catalyst recyclability confirmed that the catalyst could be recycled for three reaction runs.
30393138	0	52	dep	Composite	0:8	arg1	support					107:113	A novel catalyst support	90:113	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.	0:114	Composite of ionic liquid decorated cyclodextrin nanosponge, graphene oxide and chitosan: A novel catalyst support.
30393138	1	53	theme	ionic	197:201	arg1	CDNS-IL					245:251	CDNS-IL	245:251	CDNS-IL	245:251	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
30393138	1	53	theme	ionic	197:201	arg1	nanosponge					233:242	ionic liquid decorated cyclodextrin nanosponge	197:242	ionic liquid decorated cyclodextrin nanosponge (CDNS-IL)	197:252	A biocompatible hybrid system composed of graphene oxide (GO), chitosan (CS) and ionic liquid decorated cyclodextrin nanosponge (CDNS-IL) is synthesized and applied for the immobilization of Pd and Fe3O4 nanoparticles to furnish a magnetic catalyst, Fe3O4/Pd@Hybrid.
29900735	8	0	theme	CMC	1110:1112	arg1	experiment					1122:1131	the low CMC loading experiment	1102:1131	the low CMC loading experiment	1102:1131	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	0	1	from	Mobility	12:19	arg1	Media					107:111	Fractured Media	97:111	Fractured Media	97:111	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	6	2	theme	conservative	918:929	arg1	tracer					931:936	a conservative tracer	916:936	a conservative tracer with the CIC-CMC in both cases	916:967	Iodide was injected as a conservative tracer with the CIC-CMC in both cases.
29900735	6	2	theme	conservative	918:929	arg1	Iodide					893:898	Iodide	893:898	Iodide	893:898	Iodide was injected as a conservative tracer with the CIC-CMC in both cases.
29900735	8	3	theme	%	1210:1210	arg1	recovery					1195:1202	an additional CIC recovery	1177:1202	an additional CIC recovery of 2.5%	1177:1210	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	0	4	theme	Iron	89:92	arg1	Mobility					12:19	Field Scale Mobility	0:19	Field Scale Mobility	0:19	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	0	4	theme	Iron	89:92	arg1	Manipulation					35:46	Transport Manipulation	25:46	Transport Manipulation	25:46	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	2	5	theme	Colloids	315:322	arg1	transport					291:299	the transport	287:299	the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC),	287:437	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	9	6	from	possibility	1309:1319	arg1	environments					1288:1299	fractured environments	1278:1299	fractured environments	1278:1299	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	2	7	theme	field	456:460	arg1	conditions					462:471	field conditions	456:471	field conditions	456:471	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	8	8	theme	CIC	1191:1193	arg1	recovery					1195:1202	an additional CIC recovery	1177:1202	an additional CIC recovery of 2.5%	1177:1210	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	7	9	dep	CIC-CMC	992:998	arg1	the					988:990	the	988:990	the	988:990	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	7	9	dep	CIC-CMC	992:998	arg1	recovery					1011:1018	recovery	1011:1018	recovery	1011:1018	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	4	10	theme	borehole	734:741	arg1	up-gradient					748:758	an injection borehole 47 m up-gradient	721:758	an injection borehole 47 m up-gradient	721:758	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	1	11	theme	few	244:246	arg1	meters					248:253	a few meters	242:253	a few meters	242:253	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	0	12	theme	Fractured	97:105	arg1	Media					107:111	Fractured Media	97:111	Fractured Media	97:111	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	3	13	theme	field	478:482	arg1	site					484:487	The field site	474:487	The field site	474:487	The field site lies within a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater.
29900735	5	14	theme	%	825:825	arg1	CMC					830:832	high 100% wt CMC	817:832	high 100% wt CMC (40 g/L)	817:841	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	14	theme	%	825:825	arg1	g/L					838:840	40 g/L	835:840	40 g/L	835:840	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	2	15	theme	Carbo-Iron	304:313	arg1	material					343:350	a composite material	331:350	a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC)	331:436	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	2	15	theme	Carbo-Iron	304:313	arg1	CIC					325:327	CIC	325:327	CIC	325:327	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	2	15	theme	Carbo-Iron	304:313	arg1	Colloids					315:322	Carbo-Iron Colloids	304:322	Carbo-Iron Colloids (CIC)	304:328	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	4	16	theme	m	746:746	arg1	up-gradient					748:758	an injection borehole 47 m up-gradient	721:758	an injection borehole 47 m up-gradient	721:758	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	2	17	theme	carbon	365:370	arg1	material					343:350	a composite material	331:350	a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC)	331:436	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	2	17	theme	carbon	365:370	arg1	Colloids					315:322	Carbo-Iron Colloids	304:322	Carbo-Iron Colloids (CIC)	304:328	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	8	18	theme	pumping	1138:1144	arg1	rate					1146:1149	the pumping rate	1134:1149	the pumping rate	1134:1149	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	4	19	theme	gradient	594:601	arg1	test					610:613	A forced gradient tracer test	585:613	A forced gradient tracer test	585:613	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	8	20	theme	loading	1114:1120	arg1	experiment					1122:1131	the low CMC loading experiment	1102:1131	the low CMC loading experiment	1102:1131	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	0	21	from	Manipulation	35:46	arg1	Media					107:111	Fractured Media	97:111	Fractured Media	97:111	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	9	22	theme	fractured	1278:1286	arg1	environments					1288:1299	fractured environments	1278:1299	fractured environments	1278:1299	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	4	23	theme	L/min	674:678	arg1	rate					664:667	a rate	662:667	a rate of 8 L/min	662:678	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	2	24	theme	composite	333:341	arg1	material					343:350	a composite material	331:350	a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC)	331:436	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	2	24	theme	composite	333:341	arg1	Colloids					315:322	Carbo-Iron Colloids	304:322	Carbo-Iron Colloids (CIC)	304:328	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	0	25	theme	Scale	6:10	arg1	Mobility					12:19	Field Scale Mobility	0:19	Field Scale Mobility	0:19	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	6	26	with	tracer	931:936	arg1	CIC-CMC					947:953	the CIC-CMC	943:953	the CIC-CMC in both cases	943:967	Iodide was injected as a conservative tracer with the CIC-CMC in both cases.
29900735	5	27	theme	wt	827:828	arg1	CMC					830:832	high 100% wt CMC	817:832	high 100% wt CMC (40 g/L)	817:841	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	27	theme	wt	827:828	arg1	g/L					838:840	40 g/L	835:840	40 g/L	835:840	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	7	28	theme	CMC	1056:1058	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	1	29	theme	stabilized	174:183	arg1	nZVI					217:220	nZVI	217:220	nZVI	217:220	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	1	29	theme	stabilized	174:183	arg1	particles					206:214	stabilized nano zerovalent iron particles	174:214	stabilized nano zerovalent iron particles (nZVI)	174:221	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	0	30	theme	Field	0:4	arg1	Mobility					12:19	Field Scale Mobility	0:19	Field Scale Mobility	0:19	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	8	31	theme	low	1106:1108	arg1	experiment					1122:1131	the low CMC loading experiment	1102:1131	the low CMC loading experiment	1102:1131	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	9	32	from	mobility	1258:1265	arg1	environments					1288:1299	fractured environments	1278:1299	fractured environments	1278:1299	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	1	33	theme	nano	185:188	arg1	nZVI					217:220	nZVI	217:220	nZVI	217:220	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	1	33	theme	nano	185:188	arg1	particles					206:214	stabilized nano zerovalent iron particles	174:214	stabilized nano zerovalent iron particles (nZVI)	174:221	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	1	34	from	applications	123:134	arg1	media					154:158	porous media	147:158	porous media	147:158	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	2	35	theme	present	272:278	arg1	study					280:284	the present study	268:284	the present study	268:284	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	8	36	theme	additional	1180:1189	arg1	recovery					1195:1202	an additional CIC recovery	1177:1202	an additional CIC recovery of 2.5%	1177:1210	During the low CMC loading experiment, the pumping rate was increased, leading to an additional CIC recovery of 2.5%.
29900735	1	37	theme	zerovalent	190:199	arg1	nZVI					217:220	nZVI	217:220	nZVI	217:220	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	1	37	theme	zerovalent	190:199	arg1	particles					206:214	stabilized nano zerovalent iron particles	174:214	stabilized nano zerovalent iron particles (nZVI)	174:221	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	9	38	theme	transport	1325:1333	arg1	manipulation					1335:1346	transport manipulation	1325:1346	transport manipulation through the adjustment of stabilizer concentration and transport velocity	1325:1420	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	5	39	dep	conducted	797:805	arg1	second					849:854	second	849:854	second	849:854	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	39	dep	conducted	797:805	arg1	one					808:810	one	808:810	one	808:810	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	1	40	theme	iron	201:204	arg1	nZVI					217:220	nZVI	217:220	nZVI	217:220	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	1	40	theme	iron	201:204	arg1	particles					206:214	stabilized nano zerovalent iron particles	174:214	stabilized nano zerovalent iron particles (nZVI)	174:221	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	2	41	theme	activated	355:363	arg1	carbon					365:370	activated carbon	355:370	activated carbon	355:370	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	5	42	theme	%	868:868	arg1	loading					873:879	9% wt loading	867:879	lower 9% wt loading (∼2.7 g/L)	861:890	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	42	theme	%	868:868	arg1	g/L					887:889	∼2.7 g/L	882:889	∼2.7 g/L	882:889	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	4	43	theme	forced	587:592	arg1	test					610:613	A forced gradient tracer test	585:613	A forced gradient tracer test	585:613	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	1	44	theme	particles	206:214	arg1	transport					161:169	transport	161:169	transport of stabilized nano zerovalent iron particles (nZVI)	161:221	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	0	45	theme	Transport	25:33	arg1	Manipulation					35:46	Transport Manipulation	25:46	Transport Manipulation	25:46	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	2	46	theme	carboxymethyl	408:420	arg1	CMC					433:435	CMC	433:435	CMC	433:435	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	2	46	theme	carboxymethyl	408:420	arg1	cellulose					422:430	carboxymethyl cellulose	408:430	carboxymethyl cellulose (CMC)	408:436	In the present study, the transport of Carbo-Iron Colloids (CIC), a composite material of activated carbon as a carrier for nZVI stabilized by carboxymethyl cellulose (CMC), was tested under field conditions.
29900735	5	47	theme	wt	870:871	arg1	loading					873:879	9% wt loading	867:879	lower 9% wt loading (∼2.7 g/L)	861:890	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	47	theme	wt	870:871	arg1	g/L					887:889	∼2.7 g/L	882:889	∼2.7 g/L	882:889	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	6	48	from	CIC-CMC	947:953	arg1	cases					963:967	both cases	958:967	both cases	958:967	Iodide was injected as a conservative tracer with the CIC-CMC in both cases.
29900735	9	49	theme	high	1253:1256	arg1	mobility					1258:1265	the potentially high mobility	1237:1265	the potentially high mobility of nZVI in fractured environments	1237:1299	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	0	50	theme	Carbon-Supported	51:66	arg1	Iron					89:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	5	51	theme	field	773:777	arg1	applications					779:790	Two CIC-CMC field applications	761:790	Two CIC-CMC field applications	761:790	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	4	52	theme	injection	724:732	arg1	up-gradient					748:758	an injection borehole 47 m up-gradient	721:758	an injection borehole 47 m up-gradient	721:758	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	3	53	theme	fractured	503:511	arg1	aquitard					519:526	a fractured chalk aquitard	501:526	a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater	501:582	The field site lies within a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater.
29900735	1	54	theme	field	117:121	arg1	applications					123:134	field applications	117:134	field applications	117:134	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	3	55	dep	saline	556:561	arg1	mS					568:569	∼13 mS	564:569	∼13 mS	564:569	The field site lies within a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater.
29900735	9	56	theme	nZVI	1270:1273	arg1	mobility					1258:1265	the potentially high mobility	1237:1265	the potentially high mobility of nZVI in fractured environments	1237:1299	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	9	56	theme	nZVI	1270:1273	arg1	possibility					1309:1319	the possibility	1305:1319	the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity	1305:1420	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	7	57	from	%	1026:1026	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	3	58	theme	chalk	513:517	arg1	aquitard					519:526	a fractured chalk aquitard	501:526	a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater	501:582	The field site lies within a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater.
29900735	7	59	theme	loading	1060:1066	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	5	60	theme	high	817:820	arg1	CMC					830:832	high 100% wt CMC	817:832	high 100% wt CMC (40 g/L)	817:841	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	60	theme	high	817:820	arg1	g/L					838:840	40 g/L	835:840	40 g/L	835:840	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	9	61	theme	velocity	1413:1420	arg1	adjustment					1360:1369	the adjustment	1356:1369	the adjustment of stabilizer concentration and transport velocity	1356:1420	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	3	62	theme	saline	556:561	arg1	groundwater					572:582	moderately saline (∼13 mS) groundwater	545:582	moderately saline (∼13 mS) groundwater	545:582	The field site lies within a fractured chalk aquitard characterized by moderately saline (∼13 mS) groundwater.
29900735	9	63	theme	concentration	1385:1397	arg1	adjustment					1360:1369	the adjustment	1356:1369	the adjustment of stabilizer concentration and transport velocity	1356:1420	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	5	64	theme	100	822:824	arg1	%					825:825	%	825:825	%	825:825	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	4	65	theme	CMC-stabilized	684:697	arg1	CIC					699:701	CMC-stabilized CIC	684:701	CMC-stabilized CIC	684:701	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	4	66	theme	tracer	603:608	arg1	test					610:613	A forced gradient tracer test	585:613	A forced gradient tracer test	585:613	A forced gradient tracer test was conducted where one borehole was pumped at a rate of 8 L/min and CMC-stabilized CIC was introduced at an injection borehole 47 m up-gradient.
29900735	7	67	theme	low	1052:1054	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	0	68	theme	Zerovalent	78:87	arg1	Iron					89:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	7	69	from	%	1034:1034	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	5	70	dep	lower	861:865	arg1	loading					873:879	9% wt loading	867:879	lower 9% wt loading (∼2.7 g/L)	861:890	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	5	70	dep	lower	861:865	arg1	g/L					887:889	∼2.7 g/L	882:889	∼2.7 g/L	882:889	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	7	71	theme	high	1043:1046	arg1	experiments					1068:1078	the high and low CMC loading experiments	1039:1078	the high and low CMC loading experiments	1039:1078	The ratio between the CIC-CMC and iodide recovery was 76% and 45% in the high and low CMC loading experiments, respectively.
29900735	5	72	theme	CIC-CMC	765:771	arg1	applications					779:790	Two CIC-CMC field applications	761:790	Two CIC-CMC field applications	761:790	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	9	73	theme	stabilizer	1374:1383	arg1	concentration					1385:1397	stabilizer concentration	1374:1397	stabilizer concentration	1374:1397	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
29900735	0	74	theme	Nanoscale	68:76	arg1	Iron					89:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Carbon-Supported Nanoscale Zerovalent Iron	51:92	Field Scale Mobility and Transport Manipulation of Carbon-Supported Nanoscale Zerovalent Iron in Fractured Media.
29900735	5	75	theme	9	867:867	arg1	%					868:868	%	868:868	%	868:868	Two CIC-CMC field applications were conducted: one used high 100% wt CMC (40 g/L) and a second used lower 9% wt loading (∼2.7 g/L).
29900735	1	76	theme	porous	147:152	arg1	media					154:158	porous media	147:158	porous media	147:158	In field applications, mostly in porous media, transport of stabilized nano zerovalent iron particles (nZVI) has never exceeded a few meters in range.
29900735	9	77	theme	transport	1403:1411	arg1	velocity					1413:1420	transport velocity	1403:1420	transport velocity	1403:1420	The results demonstrate the potentially high mobility of nZVI in fractured environments and the possibility for transport manipulation through the adjustment of stabilizer concentration and transport velocity.
31730962	0	0	theme	drug	106:109	arg1	carrier					111:117	potential drug carrier	96:117	potential drug carrier	96:117	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	7	1	theme	obvious	1225:1231	arg1	toxicity					1233:1240	obvious toxicity	1225:1240	obvious toxicity against HepG2 cells	1225:1260	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	7	2	theme	in	1132:1133	arg1	cytotoxicity					1141:1152	The in vitro cytotoxicity	1128:1152	The in vitro cytotoxicity	1128:1152	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	3	3	theme	CMC	542:544	arg1	ratio					533:537	different blending ratio	514:537	different blending ratio of CMC	514:544	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	7	4	dep	loaded	1197:1202	arg1	drug					1192:1195	drug	1192:1195	drug	1192:1195	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	1	5	theme	composite	283:291	arg1	film					302:305	cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film	183:305	cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film	183:305	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	0	6	theme	potential	96:104	arg1	carrier					111:117	potential drug carrier	96:117	potential drug carrier	96:117	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	4	7	with	properties	742:751	arg1	elongation					800:809	2.3% elongation	795:809	2.3% elongation at break	795:818	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	7	with	properties	742:751	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	6	8	theme	well	1105:1108	arg1	biocompatibility					1110:1125	well biocompatibility	1105:1125	well biocompatibility	1105:1125	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	9	9	theme	drug	1531:1534	arg1	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	9	theme	drug	1531:1534	arg1	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	1	10	theme	hydrogel	293:300	arg1	film					302:305	cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film	183:305	cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film	183:305	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	3	11	theme	rheology	571:578	arg1	measurement					580:590	rheology measurement	571:590	rheology measurement	571:590	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	4	12	theme	%	772:772	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	7	13	theme	composite	1204:1212	arg1	films					1214:1218	the drug loaded composite films	1188:1218	the drug loaded composite films	1188:1218	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	1	14	link	crosslinked	215:225	arg1	chitosan					263:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	4	15	dep	showed	714:719	arg1	compared					882:889	compared	882:889	showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film	714:906	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	16	theme	excellent	721:729	arg1	properties					742:751	excellent mechanical properties	721:751	excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break)	721:819	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	7	17	theme	loaded	1197:1202	arg1	films					1214:1218	the drug loaded composite films	1188:1218	the drug loaded composite films	1188:1218	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	7	18	theme	GSH	1292:1294	arg1	presence					1280:1287	the presence	1276:1287	the presence of GSH	1276:1294	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	4	19	theme	ratio	860:864	arg1	swellability					825:836	swellability	825:836	swellability (equilibrium swelling ratio increased 270%)	825:880	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	19	theme	ratio	860:864	arg1	%					879:879	equilibrium swelling ratio increased 270%	839:879	equilibrium swelling ratio increased 270%	839:879	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	6	20	theme	pH	1053:1054	arg1	responsive					1056:1065	redox and pH responsive	1043:1065	responsive	1056:1065	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	4	21	theme	%	798:798	arg1	elongation					800:809	2.3% elongation	795:809	2.3% elongation at break	795:818	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	5	22	contain	has	960:962	arg1	5-Fluorouracil					909:922	5-Fluorouracil	909:922	5-Fluorouracil (5-FU)	909:929	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	5	22	contain	has	960:962	arg2	properties					990:999	broad-spectrum anticancer properties	964:999	broad-spectrum anticancer properties	964:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	5	22	contain	has	960:962	arg1	model					948:952	drug model	943:952	drug model which has broad-spectrum anticancer properties	943:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	6	23	theme	properties	1083:1092	arg1	responsive					1056:1065	redox and pH responsive	1043:1065	responsive	1056:1065	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	6	24	theme	redox	1043:1047	arg1	responsive					1056:1065	redox and pH responsive	1043:1065	responsive	1056:1065	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	4	25	theme	swelling	851:858	arg1	swellability					825:836	swellability	825:836	swellability (equilibrium swelling ratio increased 270%)	825:880	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	25	theme	swelling	851:858	arg1	%					879:879	equilibrium swelling ratio increased 270%	839:879	equilibrium swelling ratio increased 270%	839:879	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	26	theme	2.3	795:797	arg1	%					798:798	%	798:798	%	798:798	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	6	27	theme	release	1075:1081	arg1	properties					1083:1092	drug release properties	1070:1092	drug release properties	1070:1092	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	8	28	theme	CMC/HACC	1310:1317	arg1	films					1329:1333	CMC/HACC composite films	1310:1333	CMC/HACC composite films	1310:1333	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	8	29	contain	had	1412:1414	arg2	activities					1433:1442	no antimicrobial activities	1416:1442	no antimicrobial activities	1416:1442	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	8	29	contain	had	1412:1414	arg1	film					1407:1410	pure CMC film	1398:1410	pure CMC film	1398:1410	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	9	30	theme	targeted	1522:1529	arg1	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	30	theme	targeted	1522:1529	arg1	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	4	31	theme	93.3	768:771	arg1	%					772:772	%	772:772	%	772:772	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	6	32	theme	drug	1070:1073	arg1	properties					1083:1092	drug release properties	1070:1092	drug release properties	1070:1092	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	5	33	used	used	935:938	arg2	5-FU					925:928	5-FU	925:928	5-FU	925:928	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	5	33	used	used	935:938	arg2	5-Fluorouracil					909:922	5-Fluorouracil	909:922	5-Fluorouracil (5-FU)	909:929	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	5	33	used	used	935:938	arg2	model					948:952	drug model	943:952	drug model which has broad-spectrum anticancer properties	943:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	7	34	theme	apoptosis	1163:1171	arg1	studies					1173:1179	The in vitro cytotoxicity and cell apoptosis studies	1128:1179	The in vitro cytotoxicity and cell apoptosis studies	1128:1179	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	4	35	theme	increased	866:874	arg1	swellability					825:836	swellability	825:836	swellability (equilibrium swelling ratio increased 270%)	825:880	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	35	theme	increased	866:874	arg1	%					879:879	equilibrium swelling ratio increased 270%	839:879	equilibrium swelling ratio increased 270%	839:879	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	2	36	theme	chitosan	356:363	arg1	HACC					377:380	HACC	377:380	HACC	377:380	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	2	36	theme	chitosan	356:363	arg1	derivative					365:374	chitosan derivative	356:374	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	3	37	theme	1HNMR	473:477	arg1	spectra					479:485	1HNMR spectra	473:485	1HNMR spectra	473:485	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	4	38	theme	composite	633:641	arg1	films					643:647	the composite films	629:647	the composite films	629:647	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	39	theme	improved	759:766	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	40	theme	tensile	774:780	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	5	41	theme	drug	943:946	arg1	5-Fluorouracil					909:922	5-Fluorouracil	909:922	5-Fluorouracil (5-FU)	909:929	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	5	41	theme	drug	943:946	arg1	model					948:952	drug model	943:952	drug model which has broad-spectrum anticancer properties	943:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	4	42	theme	optimized	669:677	arg1	conditions					679:688	optimized conditions	669:688	optimized conditions (CMC: HACC = 7:1)	669:706	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	42	theme	optimized	669:677	arg1	CMC					691:693	CMC	691:693	CMC: HACC = 7:1	691:705	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	3	43	theme	swelling	608:615	arg1	tests					617:621	swelling tests	608:621	swelling tests	608:621	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	9	44	theme	CMC/HACC	1470:1477	arg1	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	44	theme	CMC/HACC	1470:1477	arg1	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	2	45	theme	quaternary	336:345	arg1	ammonium					347:354	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	1	46	theme	crosslinked	215:225	arg1	chitosan					263:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	0	47	theme	cellulose	50:58	arg1	containing					60:69	carboxymethyl cellulose containing	36:69	carboxymethyl cellulose containing	36:69	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	7	48	dep	in	1132:1133	arg1	vitro					1135:1139	vitro	1135:1139	vitro	1135:1139	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	8	49	theme	pure	1398:1401	arg1	film					1407:1410	pure CMC film	1398:1410	pure CMC film	1398:1410	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	1	50	theme	carboxymethyl	227:239	arg1	chitosan					263:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	0	51	theme	carboxymethyl	36:48	arg1	containing					60:69	carboxymethyl cellulose containing	36:69	carboxymethyl cellulose containing	36:69	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	3	52	theme	FTIR	467:470	arg1	spectra					479:485	1HNMR spectra	473:485	1HNMR spectra	473:485	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	8	53	theme	composite	1319:1327	arg1	films					1329:1333	CMC/HACC composite films	1310:1333	CMC/HACC composite films	1310:1333	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	6	54	theme	composite	1020:1028	arg1	films					1030:1034	The 5-FU/CMC/HACC composite films	1002:1034	The 5-FU/CMC/HACC composite films	1002:1034	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31730962	1	55	theme	cellulose/quaternized	241:261	arg1	chitosan					263:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	7	56	theme	cytotoxicity	1141:1152	arg1	studies					1173:1179	The in vitro cytotoxicity and cell apoptosis studies	1128:1179	The in vitro cytotoxicity and cell apoptosis studies	1128:1179	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	4	57	theme	CMC	899:901	arg1	film					903:906	pure CMC film	894:906	pure CMC film	894:906	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	58	theme	equilibrium	839:849	arg1	swellability					825:836	swellability	825:836	swellability (equilibrium swelling ratio increased 270%)	825:880	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	58	theme	equilibrium	839:849	arg1	%					879:879	equilibrium swelling ratio increased 270%	839:879	equilibrium swelling ratio increased 270%	839:879	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	2	59	dep	ammonium	347:354	arg1	HACC					377:380	HACC	377:380	HACC	377:380	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	2	59	dep	ammonium	347:354	arg1	derivative					365:374	chitosan derivative	356:374	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	0	60	theme	containing	60:69	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	0	60	theme	containing	60:69	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of carboxymethyl cellulose containing quaternized chitosan for potential drug carrier.
31730962	4	61	theme	pure	894:897	arg1	film					903:906	pure CMC film	894:906	pure CMC film	894:906	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	9	62	theme	delivery	1536:1543	arg1	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	62	theme	delivery	1536:1543	arg1	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	8	63	theme	CMC	1403:1405	arg1	film					1407:1410	pure CMC film	1398:1410	pure CMC film	1398:1410	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	4	64	theme	mechanical	731:740	arg1	properties					742:751	excellent mechanical properties	721:751	excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break)	721:819	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	8	65	theme	antimicrobial	1419:1431	arg1	activities					1433:1442	no antimicrobial activities	1416:1442	no antimicrobial activities	1416:1442	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	5	66	theme	anticancer	979:988	arg1	properties					990:999	broad-spectrum anticancer properties	964:999	broad-spectrum anticancer properties	964:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	1	67	theme	study	142:146	arg1	objective					124:132	The objective	120:132	The objective of this study	120:146	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	5	68	theme	broad-spectrum	964:977	arg1	properties					990:999	broad-spectrum anticancer properties	964:999	broad-spectrum anticancer properties	964:999	5-Fluorouracil (5-FU) was used as drug model which has broad-spectrum anticancer properties.
31730962	1	69	dep	composite	283:291	arg1	chitosan					263:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	crosslinked carboxymethyl cellulose/quaternized chitosan	215:270	The objective of this study was to synthesize and characterize cystamine dihydrochloride (CYS) crosslinked carboxymethyl cellulose/quaternized chitosan (CMC/HACC) composite hydrogel film.
31730962	2	70	theme	glycidyl-based	321:334	arg1	ammonium					347:354	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	the glycidyl-based quaternary ammonium chitosan derivative (HACC)	317:381	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	7	71	theme	cell	1158:1161	arg1	apoptosis					1163:1171	cell apoptosis	1158:1171	cell apoptosis	1158:1171	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	3	72	theme	blending	524:531	arg1	ratio					533:537	different blending ratio	514:537	different blending ratio of CMC	514:544	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	8	73	theme	good	1342:1345	arg1	antibacterial					1347:1359	good antibacterial	1342:1359	good antibacterial	1342:1359	In addition, CMC/HACC composite films showed good antibacterial against S. aureus and E. coli, while pure CMC film had no antimicrobial activities.
31730962	3	74	theme	different	514:522	arg1	ratio					533:537	different blending ratio	514:537	different blending ratio of CMC	514:544	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	9	75	theme	composite	1479:1487	arg1	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	75	theme	composite	1479:1487	arg1	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	3	76	theme	composite	495:503	arg1	films					505:509	the composite films	491:509	the composite films	491:509	Then HACC was analyzed by FTIR, 1HNMR spectra and the composite films at different blending ratio of CMC and HACC were studied by rheology measurement, mechanical and swelling tests.
31730962	4	77	from	break	814:818	arg1	elongation					800:809	2.3% elongation	795:809	2.3% elongation at break	795:818	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	77	from	break	814:818	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	2	78	theme	substitution	418:429	arg1	reaction					431:438	nucleophilic substitution reaction	405:438	nucleophilic substitution reaction	405:438	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	9	79	used	used	1514:1517	arg2	films					1489:1493	CMC/HACC composite films	1470:1493	CMC/HACC composite films	1470:1493	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	9	79	used	used	1514:1517	arg2	system					1545:1550	targeted drug delivery system	1522:1550	targeted drug delivery system	1522:1550	It can be concluded that CMC/HACC composite films can be potentially used as targeted drug delivery system.
31730962	4	80	with	swellability	825:836	arg1	elongation					800:809	2.3% elongation	795:809	2.3% elongation at break	795:818	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	4	80	with	swellability	825:836	arg1	strength					782:789	improved 93.3% tensile strength	759:789	improved 93.3% tensile strength	759:789	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	7	81	theme	HepG2	1250:1254	arg1	cells					1256:1260	HepG2 cells	1250:1260	HepG2 cells	1250:1260	The in vitro cytotoxicity and cell apoptosis studies showed the drug loaded composite films with obvious toxicity against HepG2 cells especially in the presence of GSH.
31730962	4	82	dep	CMC	691:693	arg1	HACC = 7:1					696:705	HACC = 7:1	696:705	CMC: HACC = 7:1	691:705	When the composite films were prepared under optimized conditions (CMC: HACC = 7:1), they showed excellent mechanical properties (with improved 93.3% tensile strength and 2.3% elongation at break) and swellability (equilibrium swelling ratio increased 270%) compared to pure CMC film.
31730962	2	83	theme	nucleophilic	405:416	arg1	reaction					431:438	nucleophilic substitution reaction	405:438	nucleophilic substitution reaction	405:438	Firstly, the glycidyl-based quaternary ammonium chitosan derivative (HACC) was synthesized using nucleophilic substitution reaction.
31730962	6	84	theme	5-FU/CMC/HACC	1006:1018	arg1	films					1030:1034	The 5-FU/CMC/HACC composite films	1002:1034	The 5-FU/CMC/HACC composite films	1002:1034	The 5-FU/CMC/HACC composite films showed redox and pH responsive of drug release properties along with well biocompatibility.
31260774	1	0	theme	purified	146:153	arg1	form					155:158	the highly purified form	135:158	the highly purified form of cellulose	135:171	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	1	theme	plant	254:258	arg1	cellulose					260:268	plant cellulose	254:268	plant cellulose (PC)	254:273	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	1	theme	plant	254:258	arg1	PC					271:272	PC	271:272	PC	271:272	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	0	2	theme	dissolved	78:86	arg1	states					88:93	dissolved states	78:93	dissolved states	78:93	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	5	3	theme	liquid-holding	1088:1101	arg1	capabilities					1103:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	8	4	theme	physiological	1378:1390	arg1	better					1413:1418	better	1413:1418	better	1413:1418	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	8	4	theme	physiological	1378:1390	arg1	features					1392:1399	The overall mechanical, thermal, and physiological features	1341:1399	The overall mechanical, thermal, and physiological features of RBC	1341:1406	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	4	5	from	BC	687:688	arg1	NMMO					728:731	NMMO	728:731	NMMO	728:731	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	5	from	BC	687:688	arg1	N-oxide					719:725	N-methylmorpholine N-oxide	700:725	N-methylmorpholine N-oxide (NMMO)	700:732	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	8	6	theme	RBC	1404:1406	arg1	better					1413:1418	better	1413:1418	better	1413:1418	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	8	6	theme	RBC	1404:1406	arg1	features					1392:1399	The overall mechanical, thermal, and physiological features	1341:1399	The overall mechanical, thermal, and physiological features of RBC	1341:1406	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	6	7	theme	crystalline	1170:1180	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	5	8	theme	X-ray	999:1003	arg1	microscopy					987:996	field-emission scanning electron microscopy	954:996	field-emission scanning electron microscopy	954:996	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	8	theme	X-ray	999:1003	arg1	diffraction					1005:1015	X-ray diffraction	999:1015	X-ray diffraction	999:1015	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	4	9	theme	regenerated	757:767	arg1	cellulose					842:850	regenerated plant cellulose	824:850	regenerated plant cellulose (RPC)	824:856	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	9	theme	regenerated	757:767	arg1	cellulose					804:812	bacterial cellulose	794:812	bacterial cellulose (RBC)	794:818	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	9	theme	regenerated	757:767	arg1	gels					769:772	their regenerated gels	751:772	their regenerated gels	751:772	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	3	10	from	structures	552:561	arg1	degree					524:529	the degree	520:529	the degree of variations in the structures and properties of both PC and BC	520:594	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	5	11	theme	RPC	917:919	arg1	features					888:895	The structural features	873:895	The structural features of BC, PC, RBC, and RPC	873:919	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	9	12	from	use	1473:1475	arg1	synthesis					1497:1505	composite synthesis	1487:1505	composite synthesis for various applications	1487:1530	These findings may facilitate the use of RBC in composite synthesis for various applications.
31260774	3	13	from	degree	524:529	arg1	properties					567:576	properties	567:576	properties	567:576	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	3	13	from	degree	524:529	arg1	structures					552:561	structures	552:561	structures	552:561	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	5	14	theme	structural	877:886	arg1	features					888:895	The structural features	873:895	The structural features of BC, PC, RBC, and RPC	873:919	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	15	theme	PC	904:905	arg1	features					888:895	The structural features	873:895	The structural features of BC, PC, RBC, and RPC	873:919	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	16	theme	mechanical	1063:1072	arg1	capabilities					1103:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	4	17	theme	plant	836:840	arg1	cellulose					842:850	regenerated plant cellulose	824:850	regenerated plant cellulose (RPC)	824:856	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	17	theme	plant	836:840	arg1	gels					769:772	their regenerated gels	751:772	their regenerated gels	751:772	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	17	theme	plant	836:840	arg1	RPC					853:855	RPC	853:855	RPC	853:855	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	3	18	theme	variations	534:543	arg1	degree					524:529	the degree	520:529	the degree of variations in the structures and properties of both PC and BC	520:594	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	2	19	theme	native	399:404	arg1	cellulose					406:414	native cellulose	399:414	native cellulose	399:414	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	4	20	theme	regenerated	824:834	arg1	cellulose					842:850	regenerated plant cellulose	824:850	regenerated plant cellulose (RPC)	824:856	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	20	theme	regenerated	824:834	arg1	gels					769:772	their regenerated gels	751:772	their regenerated gels	751:772	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	20	theme	regenerated	824:834	arg1	RPC					853:855	RPC	853:855	RPC	853:855	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	6	21	theme	inferior	1136:1143	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	8	22	theme	mechanical	1353:1362	arg1	better					1413:1418	better	1413:1418	better	1413:1418	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	8	22	theme	mechanical	1353:1362	arg1	features					1392:1399	The overall mechanical, thermal, and physiological features	1341:1399	The overall mechanical, thermal, and physiological features of RBC	1341:1406	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	1	23	theme	cellulose	163:171	arg1	form					155:158	the highly purified form	135:158	the highly purified form of cellulose	135:171	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	2	24	theme	physico-mechanical	452:469	arg1	features					471:478	varied structural and physico-mechanical features	430:478	varied structural and physico-mechanical features	430:478	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	5	25	theme	thermal	1075:1081	arg1	capabilities					1103:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	their mechanical, thermal, and liquid-holding capabilities	1057:1114	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	7	26	theme	water	1277:1281	arg1	capability					1294:1303	water absorption capability	1277:1303	water absorption capability	1277:1303	However, RBC showed better porosity, water absorption capability, and water retention time than RPC.
31260774	0	27	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of plant and bacterial cellulose	0:49	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	1	28	theme	Bacterial	96:104	arg1	BC					117:118	BC	117:118	BC	117:118	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	28	theme	Bacterial	96:104	arg1	cellulose					106:114	Bacterial cellulose	96:114	Bacterial cellulose (BC)	96:119	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	2	29	theme	original	376:383	arg1	properties					385:394	the original properties	372:394	the original properties of native cellulose	372:414	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	0	30	theme	plant	21:25	arg1	study					12:16	Comparative study	0:16	Comparative study of plant and bacterial cellulose	0:49	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	7	31	theme	water	1310:1314	arg1	time					1326:1329	water retention time	1310:1329	water retention time	1310:1329	However, RBC showed better porosity, water absorption capability, and water retention time than RPC.
31260774	2	32	theme	regeneration	289:300	arg1	process					302:308	the regeneration process	285:308	the regeneration process	285:308	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	3	33	theme	BC	593:594	arg1	properties					567:576	properties	567:576	properties	567:576	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	3	33	theme	BC	593:594	arg1	structures					552:561	structures	552:561	structures	552:561	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	1	34	theme	better	184:189	arg1	morphology					203:212	better nanofibrous morphology	184:212	better nanofibrous morphology	184:212	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	2	35	theme	varied	430:435	arg1	features					471:478	varied structural and physico-mechanical features	430:478	varied structural and physico-mechanical features	430:478	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	6	36	theme	thermal	1157:1163	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	5	37	theme	RBC	908:910	arg1	features					888:895	The structural features	873:895	The structural features of BC, PC, RBC, and RPC	873:919	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	1	38	contain	possesses	174:182	arg1	BC					117:118	BC	117:118	BC	117:118	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	38	contain	possesses	174:182	arg2	morphology					203:212	better nanofibrous morphology	184:212	better nanofibrous morphology	184:212	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	38	contain	possesses	174:182	arg1	cellulose					106:114	Bacterial cellulose	96:114	Bacterial cellulose (BC)	96:119	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	1	38	contain	possesses	174:182	arg2	properties					238:247	superior mechanical properties	218:247	superior mechanical properties	218:247	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	9	39	theme	various	1511:1517	arg1	applications					1519:1530	various applications	1511:1530	various applications	1511:1530	These findings may facilitate the use of RBC in composite synthesis for various applications.
31260774	1	40	theme	nanofibrous	191:201	arg1	morphology					203:212	better nanofibrous morphology	184:212	better nanofibrous morphology	184:212	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	0	41	theme	bacterial	31:39	arg1	cellulose					41:49	bacterial cellulose	31:49	bacterial cellulose	31:49	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	5	42	theme	BC	900:901	arg1	features					888:895	The structural features	873:895	The structural features of BC, PC, RBC, and RPC	873:919	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	6	43	theme	RBC	1194:1196	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	0	44	dep	pellicles	51:59	arg1	regenerated					61:71	regenerated	61:71	pellicles regenerated from dissolved states	51:93	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	7	45	theme	absorption	1283:1292	arg1	capability					1294:1303	water absorption capability	1277:1303	water absorption capability	1277:1303	However, RBC showed better porosity, water absorption capability, and water retention time than RPC.
31260774	6	46	theme	RPC	1202:1204	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	4	47	theme	comparative	655:665	arg1	study					667:671	a detailed comparative study	644:671	a detailed comparative study	644:671	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	2	48	theme	structural	437:446	arg1	features					471:478	varied structural and physico-mechanical features	430:478	varied structural and physico-mechanical features	430:478	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	2	49	theme	cellulose	406:414	arg1	properties					385:394	the original properties	372:394	the original properties of native cellulose	372:414	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	3	50	theme	PC	586:587	arg1	properties					567:576	properties	567:576	properties	567:576	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	3	50	theme	PC	586:587	arg1	structures					552:561	structures	552:561	structures	552:561	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	4	51	theme	N-methylmorpholine	700:717	arg1	NMMO					728:731	NMMO	728:731	NMMO	728:731	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	51	theme	N-methylmorpholine	700:717	arg1	N-oxide					719:725	N-methylmorpholine N-oxide	700:725	N-methylmorpholine N-oxide (NMMO)	700:732	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	0	52	theme	cellulose	41:49	arg1	study					12:16	Comparative study	0:16	Comparative study of plant and bacterial cellulose	0:49	Comparative study of plant and bacterial cellulose pellicles regenerated from dissolved states.
31260774	8	53	theme	thermal	1365:1371	arg1	better					1413:1418	better	1413:1418	better	1413:1418	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	8	53	theme	thermal	1365:1371	arg1	features					1392:1399	The overall mechanical, thermal, and physiological features	1341:1399	The overall mechanical, thermal, and physiological features of RBC	1341:1406	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	9	54	theme	RBC	1480:1482	arg1	use					1473:1475	the use	1469:1475	the use of RBC in composite synthesis for various applications	1469:1530	These findings may facilitate the use of RBC in composite synthesis for various applications.
31260774	6	55	theme	respective	1215:1224	arg1	counterparts					1226:1237	their respective counterparts	1209:1237	their respective counterparts	1209:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	2	56	theme	intermediate	326:337	arg1	structures					339:348	intermediate structures	326:348	intermediate structures	326:348	However, the regeneration process, which produces intermediate structures, significantly alters the original properties of native cellulose and result in varied structural and physico-mechanical features.
31260774	5	57	theme	electron	978:985	arg1	analyses					1027:1034	porosity analyses	1018:1034	porosity analyses	1018:1034	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	57	theme	electron	978:985	arg1	microscopy					987:996	field-emission scanning electron microscopy	954:996	field-emission scanning electron microscopy	954:996	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	57	theme	electron	978:985	arg1	diffraction					1005:1015	X-ray diffraction	999:1015	X-ray diffraction	999:1015	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	58	theme	porosity	1018:1025	arg1	analyses					1027:1034	porosity analyses	1018:1034	porosity analyses	1018:1034	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	58	theme	porosity	1018:1025	arg1	microscopy					987:996	field-emission scanning electron microscopy	954:996	field-emission scanning electron microscopy	954:996	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	4	59	theme	regenerated	782:792	arg1	RBC					815:817	RBC	815:817	RBC	815:817	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	59	theme	regenerated	782:792	arg1	cellulose					804:812	bacterial cellulose	794:812	bacterial cellulose (RBC)	794:818	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	59	theme	regenerated	782:792	arg1	gels					769:772	their regenerated gels	751:772	their regenerated gels	751:772	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	8	60	theme	overall	1345:1351	arg1	better					1413:1418	better	1413:1418	better	1413:1418	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	8	60	theme	overall	1345:1351	arg1	features					1392:1399	The overall mechanical, thermal, and physiological features	1341:1399	The overall mechanical, thermal, and physiological features of RBC	1341:1406	The overall mechanical, thermal, and physiological features of RBC were better than those of RPC.
31260774	3	61	from	properties	567:576	arg1	degree					524:529	the degree	520:529	the degree of variations in the structures and properties of both PC and BC	520:594	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	1	62	theme	superior	218:225	arg1	properties					238:247	superior mechanical properties	218:247	superior mechanical properties	218:247	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	5	63	theme	field-emission	954:967	arg1	analyses					1027:1034	porosity analyses	1018:1034	porosity analyses	1018:1034	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	63	theme	field-emission	954:967	arg1	microscopy					987:996	field-emission scanning electron microscopy	954:996	field-emission scanning electron microscopy	954:996	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	63	theme	field-emission	954:967	arg1	diffraction					1005:1015	X-ray diffraction	999:1015	X-ray diffraction	999:1015	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	6	64	theme	mechanical	1145:1154	arg1	features					1182:1189	inferior mechanical, thermal, and crystalline features	1136:1189	inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts	1136:1237	The results showed inferior mechanical, thermal, and crystalline features of RBC and RPC to their respective counterparts.
31260774	3	65	dep	structures	552:561	arg1	the					548:550	the	548:550	the	548:550	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	1	66	theme	mechanical	227:236	arg1	properties					238:247	superior mechanical properties	218:247	superior mechanical properties	218:247	Bacterial cellulose (BC), representing the highly purified form of cellulose, possesses better nanofibrous morphology and superior mechanical properties than plant cellulose (PC).
31260774	7	67	theme	better	1260:1265	arg1	porosity					1267:1274	better porosity	1260:1274	better porosity	1260:1274	However, RBC showed better porosity, water absorption capability, and water retention time than RPC.
31260774	5	68	theme	scanning	969:976	arg1	analyses					1027:1034	porosity analyses	1018:1034	porosity analyses	1018:1034	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	68	theme	scanning	969:976	arg1	microscopy					987:996	field-emission scanning electron microscopy	954:996	field-emission scanning electron microscopy	954:996	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	5	68	theme	scanning	969:976	arg1	diffraction					1005:1015	X-ray diffraction	999:1015	X-ray diffraction	999:1015	The structural features of BC, PC, RBC, and RPC were comparatively evaluated via field-emission scanning electron microscopy, X-ray diffraction, porosity analyses, as well as analyzed their mechanical, thermal, and liquid-holding capabilities.
31260774	4	69	from	PC	694:695	arg1	NMMO					728:731	NMMO	728:731	NMMO	728:731	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	69	from	PC	694:695	arg1	N-oxide					719:725	N-methylmorpholine N-oxide	700:725	N-methylmorpholine N-oxide (NMMO)	700:732	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	70	theme	detailed	646:653	arg1	study					667:671	a detailed comparative study	644:671	a detailed comparative study	644:671	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	7	71	theme	retention	1316:1324	arg1	time					1326:1329	water retention time	1310:1329	water retention time	1310:1329	However, RBC showed better porosity, water absorption capability, and water retention time than RPC.
31260774	4	72	theme	bacterial	794:802	arg1	RBC					815:817	RBC	815:817	RBC	815:817	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	72	theme	bacterial	794:802	arg1	cellulose					804:812	bacterial cellulose	794:812	bacterial cellulose (RBC)	794:818	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	4	72	theme	bacterial	794:802	arg1	gels					769:772	their regenerated gels	751:772	their regenerated gels	751:772	Herein, we conducted a detailed comparative study by dissolving BC and PC in N-methylmorpholine N-oxide (NMMO) and synthesizing their regenerated gels, namely regenerated bacterial cellulose (RBC) and regenerated plant cellulose (RPC), respectively.
31260774	9	73	theme	composite	1487:1495	arg1	synthesis					1497:1505	composite synthesis	1487:1505	composite synthesis for various applications	1487:1530	These findings may facilitate the use of RBC in composite synthesis for various applications.
31260774	3	74	from	variations	534:543	arg1	properties					567:576	properties	567:576	properties	567:576	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
31260774	3	74	from	variations	534:543	arg1	structures					552:561	structures	552:561	structures	552:561	Therefore, it is important to estimate the degree of variations in the structures and properties of both PC and BC during their regeneration.
29625218	2	0	theme	sensory	755:761	arg1	attributes					763:772	sensory attributes	755:772	sensory attributes	755:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	1	dep	18days	842:847	arg1	18					873:874	18	873:874	18	873:874	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	1	dep	18days	842:847	arg1	15					866:867	15	866:867	15	866:867	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	4	2	theme	high	983:986	arg1	LRSM					973:976	LRSM	973:976	LRSM	973:976	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	4	2	theme	high	983:986	arg1	polysaccharide					1018:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	2	3	from	information	292:302	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	4	theme	total	791:795	arg1	acceptability					797:809	total acceptability	791:809	total acceptability	791:809	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	5	theme	total	586:590	arg1	pathogens					575:583	microbial pathogens	565:583	microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi)	565:686	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	5	theme	total	586:590	arg1	TVC					606:608	TVC	606:608	TVC	606:608	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	5	theme	total	586:590	arg1	count					599:603	total viable count	586:603	total viable count (TVC)	586:609	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	6	theme	chemical	689:696	arg1	changes					698:704	chemical changes	689:704	chemical changes (thiobarbituric acid, peroxide value and pH)	689:749	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	6	theme	chemical	689:696	arg1	acid					722:725	thiobarbituric acid	707:725	thiobarbituric acid	707:725	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	6	theme	chemical	689:696	arg1	value					737:741	peroxide value	728:741	peroxide value	728:741	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	6	theme	chemical	689:696	arg1	pH					747:748	pH	747:748	pH	747:748	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	aureus					670:675	Staphylococcus aureus	655:675	Staphylococcus aureus	655:675	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	count					599:603	total viable count	586:603	total viable count (TVC)	586:609	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	fungi					681:685	fungi	681:685	fungi	681:685	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	TVC					606:608	TVC	606:608	TVC	606:608	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	coli					649:652	Escherichia coli	637:652	Escherichia coli	637:652	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	pathogens					575:583	microbial pathogens	565:583	microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi)	565:686	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	7	dep	pathogens	575:583	arg1	bacteria					627:634	psychrotrophic bacteria	612:634	psychrotrophic bacteria	612:634	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	8	theme	pathogens	575:583	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	7	9	theme	slight	1553:1558	arg1	changes					1560:1566	slight changes	1553:1566	slight changes	1553:1566	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	2	10	from	color	775:779	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	11	from	odor	782:785	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	12	theme	LRSM+1	1358:1363	arg1	AHEO					1366:1369	LRSM+1% AHEO	1358:1369	LRSM+1% AHEO	1358:1369	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	12	theme	LRSM+1	1358:1363	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	2	13	theme	edible	533:538	arg1	coating					540:546	effect LRSM edible coating	521:546	effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	521:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	14	from	coating	540:546	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	3	15	theme	%	932:932	arg1	protein					934:940	3.86% protein	928:940	3.86% protein	928:940	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	7	16	from	TVC	1532:1534	arg1	one					1545:1547	first one	1539:1547	first one	1539:1547	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	2	17	theme	effect	521:526	arg1	coating					540:546	effect LRSM edible coating	521:546	effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	521:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	5	18	theme	antioxidant	1177:1187	arg1	IC50					1199:1202	IC50	1199:1202	IC50	1199:1202	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	5	18	theme	antioxidant	1177:1187	arg1	activity					1189:1196	antioxidant activity	1177:1196	antioxidant activity (IC50)	1177:1203	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	4	19	theme	1.19×106Da	1006:1015	arg1	LRSM					973:976	LRSM	973:976	LRSM	973:976	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	4	19	theme	1.19×106Da	1006:1015	arg1	polysaccharide					1018:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	0	20	theme	molecular	76:84	arg1	weight					86:91	molecular weight	76:91	molecular weight	76:91	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	2	21	theme	total	470:474	arg1	content					483:489	total phenol content	470:489	total phenol content	470:489	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	22	theme	LRSM+1.5	1372:1379	arg1	%					1380:1380	%	1380:1380	%	1380:1380	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	2	23	theme	viable	592:597	arg1	pathogens					575:583	microbial pathogens	565:583	microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi)	565:686	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	23	theme	viable	592:597	arg1	TVC					606:608	TVC	606:608	TVC	606:608	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	23	theme	viable	592:597	arg1	count					599:603	total viable count	586:603	total viable count (TVC)	586:609	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	24	theme	shelf	1307:1311	arg1	6					1410:1410	6	1410:1410	6	1410:1410	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	24	theme	shelf	1307:1311	arg1	life					1313:1316	the beef shelf life	1298:1316	the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1298:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	5	25	theme	total	1150:1154	arg1	equal					1210:1214	equal	1210:1214	equal	1210:1214	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	5	25	theme	total	1150:1154	arg1	content					1165:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	2	26	theme	psychrotrophic	612:625	arg1	bacteria					627:634	psychrotrophic bacteria	612:634	psychrotrophic bacteria	612:634	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	1	27	theme	whole	237:241	arg1	seeds					243:247	whole seeds	237:247	whole seeds	237:247	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	3	28	theme	76.74	907:911	arg1	%					912:912	%	912:912	%	912:912	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	2	29	from	4°C	834:836	arg1	composition					374:384	chemical composition	365:384	chemical composition (moisture, protein, ash, fat and carbohydrate)	365:431	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	color					775:779	color	775:779	color	775:779	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	odor					782:785	odor	782:785	odor	782:785	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	activity					445:452	biological activity	434:452	biological activity (antimicrobial, total phenol content and antioxidant activity)	434:515	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	information					292:302	The structural information	277:302	The structural information (monosaccharide compositions and molecular weight analysis)	277:362	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	coating					540:546	effect LRSM edible coating	521:546	effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	521:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	29	from	4°C	834:836	arg1	acceptability					797:809	total acceptability	791:809	total acceptability	791:809	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	8	30	theme	microbial	1630:1638	arg1	life					1646:1649	the microbial shelf life	1626:1649	the microbial shelf life	1626:1649	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	2	31	theme	beef	819:822	arg1	slices					824:829	the beef slices	815:829	the beef slices	815:829	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	4	32	theme	weight	998:1003	arg1	LRSM					973:976	LRSM	973:976	LRSM	973:976	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	4	32	theme	weight	998:1003	arg1	polysaccharide					1018:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	7	33	theme	%	1499:1499	arg1	AHEO					1501:1504	LRSM+1% AHEO	1493:1504	LRSM+1% AHEO	1493:1504	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	2	34	dep	moisture	387:394	arg1	carbohydrate					419:430	carbohydrate	419:430	carbohydrate	419:430	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	34	dep	moisture	387:394	arg1	fat					411:413	fat	411:413	fat	411:413	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	34	dep	moisture	387:394	arg1	ash					406:408	ash	406:408	ash	406:408	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	34	dep	moisture	387:394	arg1	protein					397:403	protein	397:403	protein	397:403	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	8	35	theme	control	1595:1601	arg1	samples					1603:1609	the control samples	1591:1609	the control samples	1591:1609	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	3	36	theme	3.86	928:931	arg1	%					932:932	%	932:932	%	932:932	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	8	37	theme	oxidative	1652:1660	arg1	stability					1662:1670	oxidative stability	1652:1670	oxidative stability	1652:1670	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	7	38	theme	significant	1462:1472	arg1	difference					1474:1483	no significant difference	1459:1483	no significant difference between LRSM+1% AHEO and LRSM samples	1459:1521	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	2	39	from	composition	374:384	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	40	theme	%	1398:1398	arg1	AHEO					1400:1403	LRSM+2% AHEO	1392:1403	LRSM+2% AHEO	1392:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	40	theme	%	1398:1398	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	1	41	dep	Lallemantia	176:186	arg1	royleana					188:195	royleana	188:195	royleana	188:195	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	1	42	theme	seed	197:200	arg1	LRSM					212:215	LRSM	212:215	LRSM	212:215	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	1	42	theme	seed	197:200	arg1	mucilage					202:209	Lallemantia royleana seed mucilage	176:209	Lallemantia royleana seed mucilage (LRSM)	176:216	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	3	43	theme	9.92	943:946	arg1	%					947:947	%	947:947	%	947:947	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	2	44	dep	composition	374:384	arg1	moisture					387:394	moisture	387:394	moisture	387:394	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	45	dep	changes	698:704	arg1	pH					747:748	pH	747:748	pH	747:748	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	45	dep	changes	698:704	arg1	acid					722:725	thiobarbituric acid	707:725	thiobarbituric acid	707:725	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	45	dep	changes	698:704	arg1	value					737:741	peroxide value	728:741	peroxide value	728:741	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	45	dep	changes	698:704	arg1	changes					698:704	chemical changes	689:704	chemical changes (thiobarbituric acid, peroxide value and pH)	689:749	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	8	46	theme	beef	1703:1706	arg1	acceptability					1686:1698	sensorial acceptability	1676:1698	sensorial acceptability	1676:1698	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	8	46	theme	beef	1703:1706	arg1	life					1646:1649	the microbial shelf life	1626:1649	the microbial shelf life	1626:1649	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	8	46	theme	beef	1703:1706	arg1	stability					1662:1670	oxidative stability	1652:1670	oxidative stability	1652:1670	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	2	47	theme	attributes	763:772	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	0	48	from	activity	105:112	arg1	beefs					154:158	beefs	154:158	beefs	154:158	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	2	49	theme	molecular	337:345	arg1	analysis					354:361	molecular weight analysis	337:361	molecular weight analysis	337:361	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	3	50	theme	%	947:947	arg1	ash					949:951	9.92% ash	943:951	9.92% ash	943:951	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	7	51	theme	LRSM	1510:1513	arg1	samples					1515:1521	LRSM samples	1510:1521	LRSM samples	1510:1521	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	3	52	theme	9.48	957:960	arg1	%					961:961	%	961:961	%	961:961	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	2	53	theme	changes	698:704	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	54	theme	structural	281:290	arg1	information					292:302	The structural information	277:302	The structural information (monosaccharide compositions and molecular weight analysis)	277:362	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	55	dep	samples	1335:1341	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	55	dep	samples	1335:1341	arg1	control					1343:1349	control	1343:1349	control	1343:1349	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	55	dep	samples	1335:1341	arg1	AHEO					1366:1369	LRSM+1% AHEO	1358:1369	LRSM+1% AHEO	1358:1369	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	55	dep	samples	1335:1341	arg1	AHEO					1400:1403	LRSM+2% AHEO	1392:1403	LRSM+2% AHEO	1392:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	55	dep	samples	1335:1341	arg1	LRSM					1352:1355	LRSM	1352:1355	LRSM	1352:1355	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	55	dep	samples	1335:1341	arg1	AHEO					1382:1385	LRSM+1.5% AHEO	1372:1385	LRSM+1.5% AHEO	1372:1385	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	2	56	theme	microbial	565:573	arg1	pathogens					575:583	microbial pathogens	565:583	microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi)	565:686	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	56	theme	microbial	565:573	arg1	count					599:603	total viable count	586:603	total viable count (TVC)	586:609	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	56	theme	microbial	565:573	arg1	fungi					681:685	fungi	681:685	fungi	681:685	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	57	theme	%	1364:1364	arg1	AHEO					1366:1369	LRSM+1% AHEO	1358:1369	LRSM+1% AHEO	1358:1369	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	57	theme	%	1364:1364	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	2	58	theme	thiobarbituric	707:720	arg1	changes					698:704	chemical changes	689:704	chemical changes (thiobarbituric acid, peroxide value and pH)	689:749	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	58	theme	thiobarbituric	707:720	arg1	acid					722:725	thiobarbituric acid	707:725	thiobarbituric acid	707:725	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	1	59	theme	Lallemantia	176:186	arg1	LRSM					212:215	LRSM	212:215	LRSM	212:215	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	1	59	theme	Lallemantia	176:186	arg1	mucilage					202:209	Lallemantia royleana seed mucilage	176:209	Lallemantia royleana seed mucilage (LRSM)	176:216	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	0	60	theme	edible	136:141	arg1	coating					143:149	edible coating	136:149	edible coating	136:149	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	7	61	theme	first	1539:1543	arg1	one					1545:1547	first one	1539:1547	first one	1539:1547	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	2	62	theme	LRSM	528:531	arg1	coating					540:546	effect LRSM edible coating	521:546	effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	521:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	63	from	acceptability	797:809	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	0	64	from	weight	86:91	arg1	beefs					154:158	beefs	154:158	beefs	154:158	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	0	65	theme	Chemical	54:61	arg1	composition					63:73	Chemical composition	54:73	Chemical composition	54:73	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	6	66	theme	LRSM+2	1392:1397	arg1	AHEO					1400:1403	LRSM+2% AHEO	1392:1403	LRSM+2% AHEO	1392:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	66	theme	LRSM+2	1392:1397	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	67	theme	%	1380:1380	arg1	samples					1335:1341	samples	1335:1341	samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1335:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	67	theme	%	1380:1380	arg1	AHEO					1382:1385	LRSM+1.5% AHEO	1372:1385	LRSM+1.5% AHEO	1372:1385	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	2	68	theme	biological	434:443	arg1	activity					445:452	biological activity	434:452	biological activity (antimicrobial, total phenol content and antioxidant activity)	434:515	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	69	from	activity	445:452	arg1	population					551:560	population	551:560	population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	551:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	70	theme	phenol	476:481	arg1	content					483:489	total phenol content	470:489	total phenol content	470:489	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	0	71	from	mucilage	44:51	arg1	beefs					154:158	beefs	154:158	beefs	154:158	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	7	72	contain	had	1549:1551	arg2	changes					1560:1566	slight changes	1553:1566	slight changes	1553:1566	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	7	72	contain	had	1549:1551	arg1	TVC					1532:1534	the TVC	1528:1534	the TVC in first one	1528:1547	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	0	73	theme	biological	94:103	arg1	activity					105:112	biological activity	94:112	biological activity	94:112	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	3	74	theme	%	912:912	arg1	carbohydrate					914:925	76.74% carbohydrate	907:925	76.74% carbohydrate	907:925	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	5	75	theme	LRSM	1145:1148	arg1	equal					1210:1214	equal	1210:1214	equal	1210:1214	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	5	75	theme	LRSM	1145:1148	arg1	content					1165:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	4	76	theme	molecular	988:996	arg1	LRSM					973:976	LRSM	973:976	LRSM	973:976	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	4	76	theme	molecular	988:996	arg1	polysaccharide					1018:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	a high molecular weight (1.19×106Da) polysaccharide	981:1031	LRSM is a high molecular weight (1.19×106Da) polysaccharide, composed of galactose (36.28%), arabinose (35.96%), rhamnose (15.18%), xylose (7.38%) and glucose (5.20%).
29625218	2	77	theme	chemical	365:372	arg1	composition					374:384	chemical composition	365:384	chemical composition (moisture, protein, ash, fat and carbohydrate)	365:431	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	6	78	theme	beef	1302:1305	arg1	6					1410:1410	6	1410:1410	6	1410:1410	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	6	78	theme	beef	1302:1305	arg1	life					1313:1316	the beef shelf life	1298:1316	the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO	1298:1403	The results showed that, the beef shelf life based on TVC for samples control, LRSM, LRSM+1% AHEO, LRSM+1.5% AHEO, and LRSM+2% AHEO were 6, 9, 9, 12, and 15days, respectively.
29625218	8	79	theme	shelf	1640:1644	arg1	life					1646:1649	the microbial shelf life	1626:1649	the microbial shelf life	1626:1649	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	2	80	theme	antioxidant	495:505	arg1	activity					507:514	antioxidant activity	495:514	antioxidant activity	495:514	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	composition					374:384	chemical composition	365:384	chemical composition (moisture, protein, ash, fat and carbohydrate)	365:431	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	color					775:779	color	775:779	color	775:779	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	odor					782:785	odor	782:785	odor	782:785	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	activity					445:452	biological activity	434:452	biological activity (antimicrobial, total phenol content and antioxidant activity)	434:515	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	information					292:302	The structural information	277:302	The structural information (monosaccharide compositions and molecular weight analysis)	277:362	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	coating					540:546	effect LRSM edible coating	521:546	effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes	521:772	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	81	theme	slices	824:829	arg1	acceptability					797:809	total acceptability	791:809	total acceptability	791:809	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	5	82	theme	phenolic	1156:1163	arg1	equal					1210:1214	equal	1210:1214	equal	1210:1214	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	5	82	theme	phenolic	1156:1163	arg1	content					1165:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content	1141:1171	The LRSM total phenolic content and antioxidant activity (IC50) were equal to 82.56±1.6μgGAE/mg and 528.54±0.35μg/ml, respectively.
29625218	2	83	dep	information	292:302	arg1	compositions					320:331	monosaccharide compositions	305:331	monosaccharide compositions	305:331	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	83	dep	information	292:302	arg1	analysis					354:361	molecular weight analysis	337:361	molecular weight analysis	337:361	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	0	84	from	composition	63:73	arg1	beefs					154:158	beefs	154:158	beefs	154:158	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	7	85	theme	LRSM+1	1493:1498	arg1	%					1499:1499	%	1499:1499	%	1499:1499	There was no significant difference between LRSM+1% AHEO and LRSM samples, but the TVC in first one had slight changes than LRSM.
29625218	1	86	theme	hot-water	255:263	arg1	extraction					265:274	hot-water extraction	255:274	hot-water extraction	255:274	In this study, Lallemantia royleana seed mucilage (LRSM) was extracted from whole seeds using hot-water extraction.
29625218	2	87	dep	activity	445:452	arg1	antimicrobial					455:467	antimicrobial	455:467	antimicrobial	455:467	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	8	88	theme	sensorial	1676:1684	arg1	acceptability					1686:1698	sensorial acceptability	1676:1698	sensorial acceptability	1676:1698	Compared to the control samples, LRSM extended the microbial shelf life, oxidative stability and sensorial acceptability of beef by 3, 6 and 6days, respectively.
29625218	2	89	theme	monosaccharide	305:318	arg1	compositions					320:331	monosaccharide compositions	305:331	monosaccharide compositions	305:331	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	90	dep	antimicrobial	455:467	arg1	activity					507:514	antioxidant activity	495:514	antioxidant activity	495:514	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	90	dep	antimicrobial	455:467	arg1	content					483:489	total phenol content	470:489	total phenol content	470:489	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	3	91	contain	had	903:905	arg2	carbohydrate					914:925	76.74% carbohydrate	907:925	76.74% carbohydrate	907:925	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	3	91	contain	had	903:905	arg2	protein					934:940	3.86% protein	928:940	3.86% protein	928:940	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	3	91	contain	had	903:905	arg1	LRSM					898:901	The LRSM	894:901	The LRSM	894:901	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	3	91	contain	had	903:905	arg2	moisture					963:970	9.48% moisture	957:970	9.48% moisture	957:970	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	3	91	contain	had	903:905	arg2	ash					949:951	9.92% ash	943:951	9.92% ash	943:951	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	0	92	from	evaluation	122:131	arg1	beefs					154:158	beefs	154:158	beefs	154:158	Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs.
29625218	3	93	theme	%	961:961	arg1	moisture					963:970	9.48% moisture	957:970	9.48% moisture	957:970	The LRSM had 76.74% carbohydrate, 3.86% protein, 9.92% ash and 9.48% moisture.
29625218	2	94	theme	peroxide	728:735	arg1	changes					698:704	chemical changes	689:704	chemical changes (thiobarbituric acid, peroxide value and pH)	689:749	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	94	theme	peroxide	728:735	arg1	value					737:741	peroxide value	728:741	peroxide value	728:741	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
29625218	2	95	theme	weight	347:352	arg1	analysis					354:361	molecular weight analysis	337:361	molecular weight analysis	337:361	The structural information (monosaccharide compositions and molecular weight analysis), chemical composition (moisture, protein, ash, fat and carbohydrate), biological activity (antimicrobial, total phenol content and antioxidant activity) and effect LRSM edible coating on population of microbial pathogens (total viable count (TVC), psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus and fungi), chemical changes (thiobarbituric acid, peroxide value and pH) and sensory attributes (color, odor and total acceptability) of the beef slices at 4°C for 18days (0, 3, 6, 9, 12, 15 and 18) were determined.
30223737	0	0	theme	tissue	121:126	arg1	engineering					128:138	bone tissue engineering	116:138	bone tissue engineering	116:138	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	4	1	theme	mechanical	637:646	arg1	strength					648:655	The mechanical strength	633:655	The mechanical strength of the scaffolds	633:672	The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds.
30223737	5	2	theme	MPa	901:903	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	3	dep	scaffolds	753:761	arg1	PU1					764:766	PU1	764:766	PU1	764:766	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	3	dep	scaffolds	753:761	arg1	scaffolds					753:761	Synthesized scaffolds	741:761	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2)	741:790	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	3	dep	scaffolds	753:761	arg1	PU2					776:778	PU2	776:778	PU2	776:778	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	3	dep	scaffolds	753:761	arg1	PUHA2					785:789	PUHA2	785:789	PUHA2	785:789	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	3	dep	scaffolds	753:761	arg1	PUHA1					769:773	PUHA1	769:773	PUHA1	769:773	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	0	4	theme	bone	116:119	arg1	engineering					128:138	bone tissue engineering	116:138	bone tissue engineering	116:138	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	8	5	theme	well-spread	1197:1207	arg1	morphology					1209:1218	well-spread morphology	1197:1218	well-spread morphology	1197:1218	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	8	6	theme	hydroxyapatite	1119:1132	arg1	addition					1107:1114	The addition	1103:1114	The addition of hydroxyapatite into CD-based polyurethane scaffolds	1103:1169	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	5	7	theme	compressive	802:812	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	8	8	theme	polyurethane	1148:1159	arg1	scaffolds					1161:1169	CD-based polyurethane scaffolds	1139:1169	CD-based polyurethane scaffolds	1139:1169	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	2	9	theme	mechanical	421:430	arg1	properties					432:441	The physicochemical and mechanical properties	397:441	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds	397:490	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated.
30223737	5	10	theme	MPa	879:881	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	8	11	theme	higher	1225:1230	arg1	proliferation					1232:1244	higher proliferation	1225:1244	higher proliferation	1225:1244	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	7	12	theme	scaffolds	1034:1042	arg1	Cytocompatibility					1003:1019	Cytocompatibility	1003:1019	Cytocompatibility of composite scaffolds	1003:1042	Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells.
30223737	9	13	theme	hydroxyapatite-polyurethane	1251:1277	arg1	scaffolds					1279:1287	The hydroxyapatite-polyurethane scaffolds	1247:1287	The hydroxyapatite-polyurethane scaffolds	1247:1287	The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
30223737	1	14	theme	β-cyclodextrin-based	209:228	arg1	polyurethane					230:241	β-cyclodextrin-based polyurethane	209:241	β-cyclodextrin-based polyurethane	209:241	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	1	15	contain	containing	179:188	arg2	hydroxyapatite					190:203	hydroxyapatite	190:203	hydroxyapatite	190:203	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	1	15	contain	containing	179:188	arg2	polyurethane					230:241	β-cyclodextrin-based polyurethane	209:241	β-cyclodextrin-based polyurethane	209:241	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	1	15	contain	containing	179:188	arg1	scaffolds					169:177	novel porous scaffolds	156:177	novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane	156:241	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	6	16	theme	pore	924:927	arg1	suitable					971:978	suitable	971:978	suitable	971:978	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	6	16	theme	pore	924:927	arg1	size					929:932	pore size	924:932	pore size	924:932	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	3	17	theme	hydroxyapatite	564:577	arg1	composites					579:588	hydroxyapatite composites	564:588	hydroxyapatite composites	564:588	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	7	18	with	interactions	1069:1080	arg1	cells					1096:1100	MC3T3-E1 cells	1087:1100	MC3T3-E1 cells	1087:1100	Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells.
30223737	4	19	from	content	719:725	arg1	scaffolds					730:738	scaffolds	730:738	scaffolds	730:738	The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds.
30223737	4	20	theme	hydroxyapatite	704:717	arg1	content					719:725	the hydroxyapatite content	700:725	the hydroxyapatite content in scaffolds	700:738	The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds.
30223737	5	21	theme	Synthesized	741:751	arg1	PU1					764:766	PU1	764:766	PU1	764:766	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	21	theme	Synthesized	741:751	arg1	scaffolds					753:761	Synthesized scaffolds	741:761	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2)	741:790	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	21	theme	Synthesized	741:751	arg1	PU2					776:778	PU2	776:778	PU2	776:778	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	21	theme	Synthesized	741:751	arg1	PUHA2					785:789	PUHA2	785:789	PUHA2	785:789	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	5	21	theme	Synthesized	741:751	arg1	PUHA1					769:773	PUHA1	769:773	PUHA1	769:773	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	2	22	theme	physicochemical	401:415	arg1	properties					432:441	The physicochemical and mechanical properties	397:441	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds	397:490	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated.
30223737	2	23	theme	scaffolds	482:490	arg1	cytocompatibility					454:470	cytocompatibility	454:470	cytocompatibility of porous scaffolds	454:490	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated.
30223737	1	24	theme	bone	372:375	arg1	engineering					384:394	bone tissue engineering	372:394	bone tissue engineering	372:394	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	5	25	theme	MPa	845:847	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	8	26	theme	cell	1180:1183	arg1	attachment					1185:1194	cell attachment	1180:1194	cell attachment	1180:1194	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	3	27	theme	cancellous	594:603	arg1	structure					622:630	cancellous bone-like porous structure	594:630	cancellous bone-like porous structure	594:630	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	1	28	theme	tissue	377:382	arg1	engineering					384:394	bone tissue engineering	372:394	bone tissue engineering	372:394	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	3	29	contain	had	590:592	arg1	polyurethane					535:546	polyurethane	535:546	polyurethane reinforced with hydroxyapatite composites	535:588	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	3	29	contain	had	590:592	arg2	structure					622:630	cancellous bone-like porous structure	594:630	cancellous bone-like porous structure	594:630	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	3	30	theme	bone-like	605:613	arg1	structure					622:630	cancellous bone-like porous structure	594:630	cancellous bone-like porous structure	594:630	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	0	31	theme	hydroxyapatite/β-cyclodextrin-based	43:77	arg1	composite					92:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	0	32	theme	porous	36:41	arg1	composite					92:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	5	33	theme	MPa	862:864	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	6	34	theme	bone	984:987	arg1	regeneration					989:1000	bone regeneration	984:1000	bone regeneration	984:1000	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	1	35	theme	polymerizing	281:292	arg1	β-cyclodextrin					294:307	polymerizing β-cyclodextrin	281:307	polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite	281:358	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	0	36	theme	composite	92:100	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	0	36	theme	composite	92:100	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	4	37	theme	scaffolds	664:672	arg1	strength					648:655	The mechanical strength	633:655	The mechanical strength of the scaffolds	633:672	The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds.
30223737	5	38	theme	strength	814:821	arg1	values					823:828	compressive strength values	802:828	compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa	802:903	Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively.
30223737	0	39	theme	polyurethane	79:90	arg1	composite					92:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	porous hydroxyapatite/β-cyclodextrin-based polyurethane composite	36:100	Preparation and characterization of porous hydroxyapatite/β-cyclodextrin-based polyurethane composite scaffolds for bone tissue engineering.
30223737	9	40	theme	bone	1325:1328	arg1	repair					1330:1335	bone repair	1325:1335	bone repair	1325:1335	The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
30223737	7	41	theme	MC3T3-E1	1087:1094	arg1	cells					1096:1100	MC3T3-E1 cells	1087:1100	MC3T3-E1 cells	1087:1100	Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells.
30223737	1	42	theme	novel	156:160	arg1	scaffolds					169:177	novel porous scaffolds	156:177	novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane	156:241	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	7	43	theme	favorable	1059:1067	arg1	interactions					1069:1080	favorable interactions	1059:1080	favorable interactions with MC3T3-E1 cells	1059:1100	Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells.
30223737	6	44	theme	scaffolds	956:964	arg1	suitable					971:978	suitable	971:978	suitable	971:978	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	6	44	theme	scaffolds	956:964	arg1	porosity					938:945	porosity	938:945	porosity	938:945	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	6	44	theme	scaffolds	956:964	arg1	size					929:932	pore size	924:932	pore size	924:932	The pore size and porosity of these scaffolds were suitable for bone regeneration.
30223737	1	45	theme	porous	162:167	arg1	scaffolds					169:177	novel porous scaffolds	156:177	novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane	156:241	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	3	46	theme	porous	615:620	arg1	structure					622:630	cancellous bone-like porous structure	594:630	cancellous bone-like porous structure	594:630	The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure.
30223737	2	47	theme	porous	475:480	arg1	scaffolds					482:490	porous scaffolds	475:490	porous scaffolds	475:490	The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated.
30223737	8	48	theme	CD-based	1139:1146	arg1	scaffolds					1161:1169	CD-based polyurethane scaffolds	1139:1169	CD-based polyurethane scaffolds	1139:1169	The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation.
30223737	1	49	theme	hexamethylene	314:326	arg1	diisocyanate					328:339	hexamethylene diisocyanate	314:339	hexamethylene diisocyanate	314:339	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	9	50	contain	have	1289:1292	arg1	scaffolds					1279:1287	The hydroxyapatite-polyurethane scaffolds	1247:1287	The hydroxyapatite-polyurethane scaffolds	1247:1287	The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
30223737	9	50	contain	have	1289:1292	arg2	potential					1298:1306	the potential to be applied in bone repair and regeneration	1294:1352	the potential to be applied in bone repair and regeneration	1294:1352	The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
30223737	1	51	with	β-cyclodextrin	294:307	arg1	hydroxyapatite					345:358	hydroxyapatite	345:358	hydroxyapatite	345:358	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	1	51	with	β-cyclodextrin	294:307	arg1	diisocyanate					328:339	hexamethylene diisocyanate	314:339	hexamethylene diisocyanate	314:339	In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering.
30223737	7	52	theme	composite	1024:1032	arg1	scaffolds					1034:1042	composite scaffolds	1024:1042	composite scaffolds	1024:1042	Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells.
29733648	8	0	theme	looser	1724:1729	arg1	material					1753:1760	looser and less birefringent material	1724:1760	looser and less birefringent material	1724:1760	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	1	1	theme	storage	277:283	arg1	modulus					285:291	a reversible and enhanced rheological storage modulus	239:291	a reversible and enhanced rheological storage modulus	239:291	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	10	2	theme	liquid	1936:1941	arg1	properties					1955:1964	liquid crystalline properties	1936:1964	liquid crystalline properties	1936:1964	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	3	3	theme	aq	710:711	arg1	concentration					713:725	the critical aq concentration	697:725	the critical aq concentration	697:725	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	6	4	theme	ITC	1139:1141	arg1	results					1143:1149	at 20 °C. ITC results	1129:1149	at 20 °C. ITC results	1129:1149	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	3	5	dep	%	657:657	arg1	wt					654:655	wt	654:655	wt	654:655	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	1	6	theme	nanocrystal	198:208	arg1	rods					226:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	1	7	theme	optical	297:303	arg1	birefringence					305:317	optical birefringence	297:317	optical birefringence	297:317	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	6	8	theme	composite	1055:1063	arg1	gels					1065:1068	the composite gels	1051:1068	the composite gels	1051:1068	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	10	9	theme	low	1969:1971	arg1	concentrations					1977:1990	low CNC concentrations	1969:1990	low CNC concentrations	1969:1990	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	8	10	with	MC	1635:1636	arg1	latter					1647:1652	latter	1647:1652	latter	1647:1652	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	8	11	theme	enthalpic	1600:1608	arg1	binding					1610:1616	enthalpic binding	1600:1616	enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material	1600:1760	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	9	12	theme	CNC	1789:1791	arg1	fragments					1800:1808	CNC chiral fragments	1789:1808	CNC chiral fragments	1789:1808	TEM, SEM, and CD indicate CNC chiral fragments within a MC/CNC composite gel.
29733648	8	13	with	CNCs	1626:1629	arg1	latter					1647:1652	latter	1647:1652	latter	1647:1652	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	6	14	theme	CNC	1181:1183	arg1	rods					1185:1188	the CNC rods	1177:1188	the CNC rods	1177:1188	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	15	theme	hollow	1362:1367	arg1	cross-sections					1369:1382	hollow cross-sections	1362:1382	hollow cross-sections	1362:1382	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	1	16	theme	colloidal	216:224	arg1	rods					226:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	7	17	theme	CNC	1495:1497	arg1	aggregates					1506:1515	the CNC chiral aggregates	1491:1515	the CNC chiral aggregates	1491:1515	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	3	18	theme	MC	750:751	arg1	concentration					756:768	the MC aq concentration	746:768	the MC aq concentration	746:768	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	8	19	theme	birefringent	1740:1751	arg1	material					1753:1760	looser and less birefringent material	1724:1760	looser and less birefringent material	1724:1760	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	4	20	theme	%	864:864	arg1	concentration					840:852	the CNC concentration	832:852	the CNC concentration of 1.5 wt %	832:864	At 20 °C, MC/CNC underwent gelation upon passing the CNC concentration of 1.5 wt %.
29733648	9	21	theme	composite	1826:1834	arg1	gel					1836:1838	a MC/CNC composite gel	1817:1838	a MC/CNC composite gel	1817:1838	TEM, SEM, and CD indicate CNC chiral fragments within a MC/CNC composite gel.
29733648	1	22	theme	cellulose	169:177	arg1	rods					226:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	162:229	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	2	23	theme	electron	524:531	arg1	microscopy					533:542	scanning and transmission electron microscopy	498:542	microscopy	533:542	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	6	24	theme	magnitude	1086:1094	arg1	order					1077:1081	an order	1074:1081	an order of magnitude	1074:1094	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	10	25	theme	hybrid	1854:1859	arg1	networks					1861:1868	MC/CNC hybrid networks	1847:1868	MC/CNC hybrid networks	1847:1868	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	6	26	theme	MC	1232:1233	arg1	chains					1235:1240	MC chains	1232:1240	MC chains	1232:1240	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	8	27	theme	aqueous	1665:1671	arg1	chains					1704:1709	molecularly dispersed polymer chains	1674:1709	molecularly dispersed polymer chains	1674:1709	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	10	28	theme	rheological	1899:1909	arg1	properties					1911:1920	tunable rheological properties	1891:1920	tunable rheological properties	1891:1920	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	8	29	theme	polymer	1696:1702	arg1	chains					1704:1709	molecularly dispersed polymer chains	1674:1709	molecularly dispersed polymer chains	1674:1709	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	3	30	from	media	627:631	arg1	concentration					594:606	The concentration	590:606	The concentration of CNCs in aqueous media	590:631	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	2	31	theme	titration	440:448	arg1	ITC					463:465	ITC	463:465	ITC	463:465	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	31	theme	titration	440:448	arg1	calorimetry					450:460	isothermal titration calorimetry	429:460	isothermal titration calorimetry (ITC)	429:466	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	0	32	theme	Inverse	0:6	arg1	Thermoreversible					8:23	Inverse Thermoreversible	0:23	Inverse Thermoreversible	0:23	Inverse Thermoreversible Mechanical Stiffening and Birefringence in a Methylcellulose/Cellulose Nanocrystal Hydrogel.
29733648	3	33	dep	keeping	665:671	arg1	i.e					660:662	i.e	660:662	i.e	660:662	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	7	34	theme	storage	1534:1540	arg1	modulus					1542:1548	enhanced storage modulus	1525:1548	enhanced storage modulus	1525:1548	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	2	35	theme	circular	469:476	arg1	dichroism					478:486	circular dichroism	469:486	circular dichroism (CD)	469:491	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	35	theme	circular	469:476	arg1	CD					489:490	CD	489:490	CD	489:490	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	6	36	theme	recent	1262:1267	arg1	studies					1269:1275	recent studies	1262:1275	recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections	1262:1382	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	2	37	dep	microscopy	533:542	arg1	TEM					553:555	TEM	553:555	TEM	553:555	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	37	dep	microscopy	533:542	arg1	SEM					545:547	SEM	545:547	SEM	545:547	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	1	38	theme	composite	131:139	arg1	hydrogels					141:149	composite hydrogels	131:149	composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods	131:229	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	0	39	theme	Methylcellulose/Cellulose	70:94	arg1	Hydrogel					108:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	Inverse Thermoreversible Mechanical Stiffening and Birefringence in a Methylcellulose/Cellulose Nanocrystal Hydrogel.
29733648	6	40	dep	ring-like	1311:1319	arg1	colloidal-scale					1322:1336	colloidal-scale	1322:1336	colloidal-scale	1322:1336	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	41	theme	persistent	1338:1347	arg1	fibrils					1349:1355	ring-like, colloidal-scale persistent fibrils	1311:1355	ring-like, colloidal-scale persistent fibrils with hollow cross-sections	1311:1382	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	2	42	theme	optical	409:415	arg1	microscopy					417:426	quantitative polarized optical microscopy	386:426	quantitative polarized optical microscopy	386:426	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	10	43	theme	MC/CNC	1847:1852	arg1	networks					1861:1868	MC/CNC hybrid networks	1847:1868	MC/CNC hybrid networks	1847:1868	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	2	44	theme	quantitative	386:397	arg1	microscopy					417:426	quantitative polarized optical microscopy	386:426	quantitative polarized optical microscopy	386:426	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	1	45	theme	rheological	265:275	arg1	modulus					285:291	a reversible and enhanced rheological storage modulus	239:291	a reversible and enhanced rheological storage modulus	239:291	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	10	46	theme	crystalline	1943:1953	arg1	properties					1955:1964	liquid crystalline properties	1936:1964	liquid crystalline properties	1936:1964	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	6	47	with	fibrils	1349:1355	arg1	cross-sections					1369:1382	hollow cross-sections	1362:1382	hollow cross-sections	1362:1382	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	4	48	theme	CNC	836:838	arg1	concentration					840:852	the CNC concentration	832:852	the CNC concentration of 1.5 wt %	832:864	At 20 °C, MC/CNC underwent gelation upon passing the CNC concentration of 1.5 wt %.
29733648	6	49	theme	°C.	1135:1137	arg1	results					1143:1149	at 20 °C. ITC results	1129:1149	at 20 °C. ITC results	1129:1149	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	8	50	theme	room	1572:1575	arg1	temperature					1577:1587	room temperature	1572:1587	room temperature	1572:1587	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	1	51	theme	cellulose	188:196	arg1	CNC					211:213	CNC	211:213	CNC	211:213	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	1	51	theme	cellulose	188:196	arg1	nanocrystal					198:208	cellulose nanocrystal	188:208	cellulose nanocrystal (CNC)	188:214	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	6	52	theme	gels	1065:1068	arg1	higher					1096:1101	higher	1096:1101	higher	1096:1101	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	52	theme	gels	1065:1068	arg1	G					1044:1044	G'	1044:1045	G'	1044:1045	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	52	theme	gels	1065:1068	arg1	modulus					1034:1040	The storage modulus	1022:1040	The storage modulus ( G') of the composite gels	1022:1068	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	10	53	theme	CNC	1973:1975	arg1	concentrations					1977:1990	low CNC concentrations	1969:1990	low CNC concentrations	1969:1990	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	3	54	theme	critical	701:708	arg1	concentration					713:725	the critical aq concentration	697:725	the critical aq concentration	697:725	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	7	55	theme	chiral	1499:1504	arg1	aggregates					1506:1515	the CNC chiral aggregates	1491:1515	the CNC chiral aggregates	1491:1515	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	10	56	with	materials	1876:1884	arg1	access					1926:1931	access	1926:1931	access to liquid crystalline properties at low CNC concentrations	1926:1990	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	10	56	with	materials	1876:1884	arg1	properties					1911:1920	tunable rheological properties	1891:1920	tunable rheological properties	1891:1920	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	9	57	theme	chiral	1793:1798	arg1	fragments					1800:1808	CNC chiral fragments	1789:1808	CNC chiral fragments	1789:1808	TEM, SEM, and CD indicate CNC chiral fragments within a MC/CNC composite gel.
29733648	3	58	theme	CNCs	611:614	arg1	concentration					594:606	The concentration	590:606	The concentration of CNCs in aqueous media	590:631	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	5	59	theme	percolative	998:1008	arg1	phenomenon					1010:1019	a percolative phenomenon	996:1019	a percolative phenomenon	996:1019	At this point, the storage modulus ( G') reached a plateau, and the birefringence underwent a stepwise increase, thus suggesting a percolative phenomenon.
29733648	9	60	theme	MC/CNC	1819:1824	arg1	gel					1836:1838	a MC/CNC composite gel	1817:1838	a MC/CNC composite gel	1817:1838	TEM, SEM, and CD indicate CNC chiral fragments within a MC/CNC composite gel.
29733648	3	61	theme	aq	753:754	arg1	concentration					756:768	the MC aq concentration	746:768	the MC aq concentration	746:768	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	5	62	theme	stepwise	961:968	arg1	increase					970:977	a stepwise increase	959:977	a stepwise increase	959:977	At this point, the storage modulus ( G') reached a plateau, and the birefringence underwent a stepwise increase, thus suggesting a percolative phenomenon.
29733648	1	63	theme	methyl	162:167	arg1	MC					180:181	MC	180:181	MC	180:181	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	1	63	theme	methyl	162:167	arg1	cellulose					169:177	methyl cellulose	162:177	methyl cellulose (MC)	162:182	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	7	64	theme	persistent	1435:1444	arg1	aggregates					1446:1455	more persistent aggregates	1430:1455	more persistent aggregates	1430:1455	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	4	65	theme	wt	861:862	arg1	%					864:864	1.5 wt %	857:864	1.5 wt %	857:864	At 20 °C, MC/CNC underwent gelation upon passing the CNC concentration of 1.5 wt %.
29733648	0	66	theme	Nanocrystal	96:106	arg1	Hydrogel					108:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	Inverse Thermoreversible Mechanical Stiffening and Birefringence in a Methylcellulose/Cellulose Nanocrystal Hydrogel.
29733648	3	67	theme	aqueous	619:625	arg1	media					627:631	aqueous media	619:631	aqueous media	619:631	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	2	68	theme	transmission	511:522	arg1	microscopy					533:542	scanning and transmission electron microscopy	498:542	microscopy	533:542	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	0	69	from	Stiffening	36:45	arg1	Hydrogel					108:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	Inverse Thermoreversible Mechanical Stiffening and Birefringence in a Methylcellulose/Cellulose Nanocrystal Hydrogel.
29733648	3	70	theme	wt	777:778	arg1	%					780:780	1.0 wt %	773:780	1.0 wt %	773:780	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	7	71	theme	MC	1419:1420	arg1	tendency					1403:1410	the tendency	1399:1410	the tendency of the MC to form more persistent aggregates	1399:1455	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	2	72	theme	scanning	498:505	arg1	microscopy					533:542	scanning and transmission electron microscopy	498:542	microscopy	533:542	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	10	73	theme	tunable	1891:1897	arg1	properties					1911:1920	tunable rheological properties	1891:1920	tunable rheological properties	1891:1920	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	2	74	used	used	563:566	arg2	ITC					463:465	ITC	463:465	ITC	463:465	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	CD					489:490	CD	489:490	CD	489:490	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	rheology					376:383	Dynamic rheology	368:383	Dynamic rheology	368:383	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	microscopy					533:542	scanning and transmission electron microscopy	498:542	microscopy	533:542	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	microscopy					417:426	quantitative polarized optical microscopy	386:426	quantitative polarized optical microscopy	386:426	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	calorimetry					450:460	isothermal titration calorimetry	429:460	isothermal titration calorimetry (ITC)	429:466	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	74	used	used	563:566	arg2	dichroism					478:486	circular dichroism	469:486	circular dichroism (CD)	469:491	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	8	75	theme	dispersed	1686:1694	arg1	chains					1704:1709	molecularly dispersed polymer chains	1674:1709	molecularly dispersed polymer chains	1674:1709	At room temperature, ITC shows enthalpic binding between CNCs and MC with the latter comprising aqueous, molecularly dispersed polymer chains that lead to looser and less birefringent material.
29733648	0	76	from	Birefringence	51:63	arg1	Hydrogel					108:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	a Methylcellulose/Cellulose Nanocrystal Hydrogel	68:115	Inverse Thermoreversible Mechanical Stiffening and Birefringence in a Methylcellulose/Cellulose Nanocrystal Hydrogel.
29733648	2	77	theme	isothermal	429:438	arg1	ITC					463:465	ITC	463:465	ITC	463:465	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	2	77	theme	isothermal	429:438	arg1	calorimetry					450:460	isothermal titration calorimetry	429:460	isothermal titration calorimetry (ITC)	429:466	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	3	78	from	concentration	594:606	arg1	media					627:631	aqueous media	619:631	aqueous media	619:631	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	5	79	theme	storage	886:892	arg1	G					904:904	G'	904:905	G'	904:905	At this point, the storage modulus ( G') reached a plateau, and the birefringence underwent a stepwise increase, thus suggesting a percolative phenomenon.
29733648	5	79	theme	storage	886:892	arg1	modulus					894:900	the storage modulus	882:900	the storage modulus ( G')	882:906	At this point, the storage modulus ( G') reached a plateau, and the birefringence underwent a stepwise increase, thus suggesting a percolative phenomenon.
29733648	1	80	dep	thermoreversibility	347:365	arg1	i.e.					333:336	i.e.	333:336	i.e.	333:336	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	3	81	theme	3.5	650:652	arg1	wt					654:655	wt	654:655	wt	654:655	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	3	82	from	CNCs	611:614	arg1	media					627:631	aqueous media	619:631	aqueous media	619:631	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29733648	7	83	theme	enhanced	1525:1532	arg1	modulus					1542:1548	enhanced storage modulus	1525:1548	enhanced storage modulus	1525:1548	Consequently, the tendency of the MC to form more persistent aggregates promotes the interactions between the CNC chiral aggregates towards enhanced storage modulus and birefringence.
29733648	6	84	dep	studies	1269:1275	arg1	collapse					1277:1284	collapse	1277:1284	collapse	1277:1284	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	10	85	from	concentrations	1977:1990	arg1	access					1926:1931	access	1926:1931	access to liquid crystalline properties at low CNC concentrations	1926:1990	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	10	85	from	concentrations	1977:1990	arg1	properties					1911:1920	tunable rheological properties	1891:1920	tunable rheological properties	1891:1920	Thus, MC/CNC hybrid networks offer materials with tunable rheological properties and access to liquid crystalline properties at low CNC concentrations.
29733648	2	86	theme	Dynamic	368:374	arg1	rheology					376:383	Dynamic rheology	368:383	Dynamic rheology	368:383	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	1	87	theme	inverse	339:345	arg1	thermoreversibility					347:365	inverse thermoreversibility	339:365	inverse thermoreversibility	339:365	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	6	88	from	temperature	1294:1304	arg1	collapse					1277:1284	collapse	1277:1284	collapse	1277:1284	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	1	89	theme	reversible	241:250	arg1	modulus					285:291	a reversible and enhanced rheological storage modulus	239:291	a reversible and enhanced rheological storage modulus	239:291	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	6	90	theme	storage	1026:1032	arg1	higher					1096:1101	higher	1096:1101	higher	1096:1101	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	90	theme	storage	1026:1032	arg1	G					1044:1044	G'	1044:1045	G'	1044:1045	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	6	90	theme	storage	1026:1032	arg1	modulus					1034:1040	The storage modulus	1022:1040	The storage modulus ( G') of the composite gels	1022:1068	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	1	91	theme	enhanced	256:263	arg1	modulus					285:291	a reversible and enhanced rheological storage modulus	239:291	a reversible and enhanced rheological storage modulus	239:291	We show that composite hydrogels comprising methyl cellulose (MC) and cellulose nanocrystal (CNC) colloidal rods display a reversible and enhanced rheological storage modulus and optical birefringence upon heating, i.e., inverse thermoreversibility.
29733648	2	92	theme	polarized	399:407	arg1	microscopy					417:426	quantitative polarized optical microscopy	386:426	quantitative polarized optical microscopy	386:426	Dynamic rheology, quantitative polarized optical microscopy, isothermal titration calorimetry (ITC), circular dichroism (CD), and scanning and transmission electron microscopy (SEM and TEM) were used for characterization.
29733648	6	93	theme	ring-like	1311:1319	arg1	fibrils					1349:1355	ring-like, colloidal-scale persistent fibrils	1311:1355	ring-like, colloidal-scale persistent fibrils with hollow cross-sections	1311:1382	The storage modulus ( G') of the composite gels was an order of magnitude higher at 60 °C compared to that at 20 °C. ITC results suggested that, at 60 °C, the CNC rods were entropically driven to interact with MC chains, which according to recent studies collapse at this temperature into ring-like, colloidal-scale persistent fibrils with hollow cross-sections.
29733648	3	94	dep	wt	654:655	arg1	to					647:648	to	647:648	to	647:648	The concentration of CNCs in aqueous media was varied up to 3.5 wt % (i.e, keeping the concentration below the critical aq concentration) while maintaining the MC aq concentration at 1.0 wt %.
29377254	8	0	theme	collagen	1094:1101	arg1	Meanwhile					1083:1091	Meanwhile	1083:1091	Meanwhile	1083:1091	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	8	0	theme	collagen	1094:1101	arg1	cross-linking					1103:1115	collagen cross-linking	1094:1115	collagen cross-linking in the hypertrophied left ventricle	1094:1151	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	13	1	theme	ventricle	1792:1800	arg1	hypertrophy					1772:1782	the compensated hypertrophy	1756:1782	the compensated hypertrophy of left ventricle	1756:1800	Therefore, the compensated hypertrophy of left ventricle gradually transited to congestive heart failure.
29377254	5	2	theme	left	630:633	arg1	structure					647:655	The left ventricular structure	626:655	The left ventricular structure	626:655	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	5	3	from	sign	706:709	arg1	hypertrophy					742:752	cardiac hypertrophy	734:752	cardiac hypertrophy	734:752	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	6	4	theme	key	862:864	arg1	enzyme					866:871	a key enzyme	860:871	a key enzyme for CD147 glycosylation	860:895	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	6	4	theme	key	862:864	arg1	V					849:849	N-acetylglucosamine transferase V	817:849	N-acetylglucosamine transferase V (GnT-V)	817:857	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	8	5	theme	hypertrophied	1124:1136	arg1	ventricle					1143:1151	the hypertrophied left ventricle	1120:1151	the hypertrophied left ventricle	1120:1151	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	12	6	theme	cross-linking	1730:1742	arg1	limitation					1707:1716	the limitation	1703:1716	the limitation of collagen cross-linking	1703:1742	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	13	7	theme	compensated	1760:1770	arg1	hypertrophy					1772:1782	the compensated hypertrophy	1756:1782	the compensated hypertrophy of left ventricle	1756:1800	Therefore, the compensated hypertrophy of left ventricle gradually transited to congestive heart failure.
29377254	7	8	theme	left	1055:1058	arg1	ventricle					1060:1068	the hypertrophied left ventricle	1037:1068	the hypertrophied left ventricle of 2K1C rat	1037:1080	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	0	9	theme	Glycosylated	0:11	arg1	CD147					13:17	Glycosylated CD147	0:17	Glycosylated CD147	0:17	Glycosylated CD147 reduces myocardial collagen cross-linking in cardiac hypertrophy.
29377254	9	10	theme	2K1C	1386:1389	arg1	hearts					1395:1400	the 2K1C rat hearts	1382:1400	the 2K1C rat hearts	1382:1400	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	10	11	gly	glycosylated	1453:1464	arg1	CD147					1466:1470	glycosylated CD147	1453:1470	glycosylated CD147 which activated MMP-2 and MMP-9	1453:1502	The above results suggested that Ang II increased glycosylated CD147 which activated MMP-2 and MMP-9.
29377254	2	12	theme	cardiac	307:313	arg1	hypertrophy					315:325	cardiac hypertrophy	307:325	cardiac hypertrophy	307:325	Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy.
29377254	7	13	theme	rat	1078:1080	arg1	ventricle					1060:1068	the hypertrophied left ventricle	1037:1068	the hypertrophied left ventricle of 2K1C rat	1037:1080	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	4	14	theme	2K1C	615:618	arg1	rats					620:623	2K1C rats	615:623	2K1C rats	615:623	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	4	15	dep	expression	574:583	arg1	the					570:572	the	570:572	the	570:572	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	8	16	theme	2K1C	1178:1181	arg1	rats					1183:1186	2K1C rats	1178:1186	2K1C rats	1178:1186	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	12	17	theme	pressure	1671:1678	arg1	overload					1680:1687	chronic pressure overload	1663:1687	chronic pressure overload	1663:1687	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	9	18	theme	collagen	1356:1363	arg1	cross-linking					1365:1377	collagen cross-linking	1356:1377	collagen cross-linking	1356:1377	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	5	19	theme	rats	791:794	arg1	weeks					777:781	16 weeks	774:781	16 weeks of 2K1C rats	774:794	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	7	20	theme	glycosylated	952:963	arg1	CD147					965:969	glycosylated CD147	952:969	glycosylated CD147	952:969	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	1	21	theme	overload-induced	135:150	arg1	hypertrophy					160:170	chronic pressure overload-induced cardiac hypertrophy	118:170	chronic pressure overload-induced cardiac hypertrophy	118:170	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	2	22	from	transition	276:285	arg1	end-stage					294:302	the end-stage	290:302	the end-stage of cardiac hypertrophy	290:325	Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy.
29377254	7	23	theme	matrix	986:991	arg1	metalloproteinase-2/-9					993:1014	matrix metalloproteinase-2/-9	986:1014	matrix metalloproteinase-2/-9 (MMP-2 and MMP-9)	986:1032	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	9	24	gly	glycosylation	1268:1280	arg1	contrary					1196:1203	the contrary	1192:1203	the contrary	1192:1203	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	24	gly	glycosylation	1268:1280	arg1	MMP-9					1317:1321	MMP-9	1317:1321	MMP-9	1317:1321	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	24	gly	glycosylation	1268:1280	arg1	MMP-2					1307:1311	MMP-2	1307:1311	MMP-2	1307:1311	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	24	gly	glycosylation	1268:1280	arg1	CD147					1285:1289	CD147	1285:1289	CD147	1285:1289	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	2	25	theme	important	241:249	arg1	factor					251:256	an important factor	238:256	an important factor that mediates the transition in the end-stage of cardiac hypertrophy	238:325	Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy.
29377254	2	25	theme	important	241:249	arg1	Angiotensin					207:217	Angiotensin II	207:220	Angiotensin II (Ang II)	207:229	Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy.
29377254	7	26	theme	GnT-V	927:931	arg1	acid					907:910	Retinoic acid	898:910	Retinoic acid	898:910	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	7	26	theme	GnT-V	927:931	arg1	agonist					916:922	an agonist	913:922	an agonist of GnT-V	913:931	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	1	27	theme	heart	175:179	arg1	failure					181:187	heart failure	175:187	heart failure	175:187	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	5	28	theme	heart	757:761	arg1	failure					763:769	heart failure	757:769	heart failure	757:769	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	9	29	theme	biosynthesis	1244:1255	arg1	tunicamycin					1206:1216	tunicamycin	1206:1216	tunicamycin	1206:1216	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	29	theme	biosynthesis	1244:1255	arg1	inhibitor					1222:1230	an inhibitor	1219:1230	an inhibitor of N-glycan biosynthesis	1219:1255	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	3	30	theme	Goldblatt	350:358	arg1	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	4	31	theme	concentric	488:497	arg1	hypertrophy					499:509	the concentric hypertrophy	484:509	the concentric hypertrophy of left ventricle	484:527	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	12	32	theme	hypertrophied	1605:1617	arg1	ventricle					1624:1632	the hypertrophied left ventricle	1601:1632	the hypertrophied left ventricle	1601:1632	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	3	33	theme	Ang	418:420	arg1	hypertension					433:444	Ang II-induced hypertension	418:444	Ang II-induced hypertension	418:444	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	7	34	theme	Retinoic	898:905	arg1	acid					907:910	Retinoic acid	898:910	Retinoic acid	898:910	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	7	34	theme	Retinoic	898:905	arg1	agonist					916:922	an agonist	913:922	an agonist of GnT-V	913:931	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	3	35	theme	2K1C	381:384	arg1	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	5	36	theme	2K1C	786:789	arg1	rats					791:794	2K1C rats	786:794	2K1C rats	786:794	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	1	37	theme	transition	102:111	arg1	mechanism					89:97	The mechanism	85:97	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure	85:187	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	1	37	theme	transition	102:111	arg1	unclear					198:204	unclear	198:204	unclear	198:204	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	3	38	theme	one-clip	371:378	arg1	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	4	39	theme	cardiac	533:539	arg1	fibrosis					541:548	cardiac fibrosis	533:548	cardiac fibrosis	533:548	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	1	40	theme	chronic	118:124	arg1	hypertrophy					160:170	chronic pressure overload-induced cardiac hypertrophy	118:170	chronic pressure overload-induced cardiac hypertrophy	118:170	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	13	41	theme	congestive	1825:1834	arg1	failure					1842:1848	congestive heart failure	1825:1848	congestive heart failure	1825:1848	Therefore, the compensated hypertrophy of left ventricle gradually transited to congestive heart failure.
29377254	0	42	theme	collagen	38:45	arg1	cross-linking					47:59	myocardial collagen cross-linking	27:59	myocardial collagen cross-linking	27:59	Glycosylated CD147 reduces myocardial collagen cross-linking in cardiac hypertrophy.
29377254	6	43	theme	transferase	837:847	arg1	enzyme					866:871	a key enzyme	860:871	a key enzyme for CD147 glycosylation	860:895	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	6	43	theme	transferase	837:847	arg1	GnT-V					852:856	GnT-V	852:856	GnT-V	852:856	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	6	43	theme	transferase	837:847	arg1	V					849:849	N-acetylglucosamine transferase V	817:849	N-acetylglucosamine transferase V (GnT-V)	817:857	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	3	44	theme	rat	387:389	arg1	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	4	45	theme	ventricle	519:527	arg1	fibrosis					541:548	cardiac fibrosis	533:548	cardiac fibrosis	533:548	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	4	45	theme	ventricle	519:527	arg1	hypertrophy					499:509	the concentric hypertrophy	484:509	the concentric hypertrophy of left ventricle	484:527	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	10	46	theme	glycosylated	1453:1464	arg1	CD147					1466:1470	glycosylated CD147	1453:1470	glycosylated CD147 which activated MMP-2 and MMP-9	1453:1502	The above results suggested that Ang II increased glycosylated CD147 which activated MMP-2 and MMP-9.
29377254	11	47	theme	collagen	1568:1575	arg1	cross-linking					1577:1589	collagen cross-linking	1568:1589	collagen cross-linking	1568:1589	Collagens were degraded by the activated MMPs and then reduced collagen cross-linking.
29377254	6	48	theme	CD147	877:881	arg1	glycosylation					883:895	CD147 glycosylation	877:895	CD147 glycosylation	877:895	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	13	49	theme	left	1787:1790	arg1	ventricle					1792:1800	left ventricle	1787:1800	left ventricle	1787:1800	Therefore, the compensated hypertrophy of left ventricle gradually transited to congestive heart failure.
29377254	7	50	theme	2K1C	1073:1076	arg1	rat					1078:1080	2K1C rat	1073:1080	2K1C rat	1073:1080	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	1	51	from	mechanism	89:97	arg1	hypertrophy					160:170	chronic pressure overload-induced cardiac hypertrophy	118:170	chronic pressure overload-induced cardiac hypertrophy	118:170	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	12	52	theme	collagen	1721:1728	arg1	cross-linking					1730:1742	collagen cross-linking	1721:1742	collagen cross-linking	1721:1742	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	5	53	theme	ventricular	635:645	arg1	structure					647:655	The left ventricular structure	626:655	The left ventricular structure	626:655	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	9	54	theme	cross-linking	1365:1377	arg1	stable					1346:1351	stable	1346:1351	stable	1346:1351	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	7	55	dep	metalloproteinase-2/-9	993:1014	arg1	MMP-9					1027:1031	MMP-9	1027:1031	MMP-9	1027:1031	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	7	55	dep	metalloproteinase-2/-9	993:1014	arg1	MMP-2					1017:1021	MMP-2	1017:1021	MMP-2	1017:1021	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	7	56	theme	hypertrophied	1041:1053	arg1	ventricle					1060:1068	the hypertrophied left ventricle	1037:1068	the hypertrophied left ventricle of 2K1C rat	1037:1080	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	2	57	theme	hypertrophy	315:325	arg1	end-stage					294:302	the end-stage	290:302	the end-stage of cardiac hypertrophy	290:325	Angiotensin II (Ang II) may be an important factor that mediates the transition in the end-stage of cardiac hypertrophy.
29377254	9	58	theme	rat	1391:1393	arg1	hearts					1395:1400	the 2K1C rat hearts	1382:1400	the 2K1C rat hearts	1382:1400	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	8	59	theme	left	1138:1141	arg1	ventricle					1143:1151	the hypertrophied left ventricle	1120:1151	the hypertrophied left ventricle	1120:1151	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	4	60	theme	elevated	451:458	arg1	Ang					460:462	The elevated Ang II	447:465	The elevated Ang II	447:465	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	7	61	gly	glycosylated	952:963	arg1	CD147					965:969	glycosylated CD147	952:969	glycosylated CD147	952:969	Retinoic acid, an agonist of GnT-V, further increased glycosylated CD147, and activated matrix metalloproteinase-2/-9 (MMP-2 and MMP-9) in the hypertrophied left ventricle of 2K1C rat.
29377254	1	62	theme	pressure	126:133	arg1	hypertrophy					160:170	chronic pressure overload-induced cardiac hypertrophy	118:170	chronic pressure overload-induced cardiac hypertrophy	118:170	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	1	63	theme	cardiac	152:158	arg1	hypertrophy					160:170	chronic pressure overload-induced cardiac hypertrophy	118:170	chronic pressure overload-induced cardiac hypertrophy	118:170	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	12	64	theme	chronic	1663:1669	arg1	overload					1680:1687	chronic pressure overload	1663:1687	chronic pressure overload	1663:1687	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	5	65	theme	cardiac	734:740	arg1	hypertrophy					742:752	cardiac hypertrophy	734:752	cardiac hypertrophy	734:752	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	5	66	from	hypertrophy	742:752	arg1	transition					718:727	the transition	714:727	the transition from cardiac hypertrophy to heart failure	714:769	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	5	66	from	hypertrophy	742:752	arg1	sign					706:709	a sign	704:709	a sign of the transition from cardiac hypertrophy to heart failure	704:769	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	3	67	theme	present	335:341	arg1	study					343:347	the present study	331:347	the present study	331:347	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	4	68	from	glycosylation	589:601	arg1	rats					620:623	2K1C rats	615:623	2K1C rats	615:623	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	9	69	theme	N-glycan	1235:1242	arg1	biosynthesis					1244:1255	N-glycan biosynthesis	1235:1255	N-glycan biosynthesis	1235:1255	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	4	70	from	expression	574:583	arg1	rats					620:623	2K1C rats	615:623	2K1C rats	615:623	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	4	71	gly	glycosylation	589:601	arg1	rats					620:623	2K1C rats	615:623	2K1C rats	615:623	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	4	71	gly	glycosylation	589:601	arg1	CD147					606:610	CD147	606:610	CD147	606:610	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	3	72	theme	two-kidney	360:369	arg1	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	13	73	theme	heart	1836:1840	arg1	failure					1842:1848	congestive heart failure	1825:1848	congestive heart failure	1825:1848	Therefore, the compensated hypertrophy of left ventricle gradually transited to congestive heart failure.
29377254	5	74	theme	transition	718:727	arg1	sign					706:709	a sign	704:709	a sign of the transition from cardiac hypertrophy to heart failure	704:769	The left ventricular structure and function detected by echocardiogram showed a sign of the transition from cardiac hypertrophy to heart failure in 16 weeks of 2K1C rats.
29377254	10	75	theme	above	1407:1411	arg1	results					1413:1419	The above results	1403:1419	The above results	1403:1419	The above results suggested that Ang II increased glycosylated CD147 which activated MMP-2 and MMP-9.
29377254	3	76	theme	II-induced	422:431	arg1	hypertension					433:444	Ang II-induced hypertension	418:444	Ang II-induced hypertension	418:444	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	9	77	theme	CD147	1285:1289	arg1	glycosylation					1268:1280	glycosylation	1268:1280	glycosylation of CD147	1268:1289	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	77	theme	CD147	1285:1289	arg1	activity					1295:1302	activity	1295:1302	activity of MMP-2 and MMP-9	1295:1321	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	1	78	from	hypertrophy	160:170	arg1	transition					102:111	transition	102:111	transition from chronic pressure overload-induced cardiac hypertrophy to heart failure	102:187	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	1	78	from	hypertrophy	160:170	arg1	mechanism					89:97	The mechanism	85:97	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure	85:187	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	1	78	from	hypertrophy	160:170	arg1	unclear					198:204	unclear	198:204	unclear	198:204	The mechanism of transition from chronic pressure overload-induced cardiac hypertrophy to heart failure is still unclear.
29377254	12	79	theme	left	1619:1622	arg1	ventricle					1624:1632	the hypertrophied left ventricle	1601:1632	the hypertrophied left ventricle	1601:1632	Finally, the hypertrophied left ventricle was progressively dilated in chronic pressure overload due to losing the limitation of collagen cross-linking.
29377254	0	80	theme	myocardial	27:36	arg1	cross-linking					47:59	myocardial collagen cross-linking	27:59	myocardial collagen cross-linking	27:59	Glycosylated CD147 reduces myocardial collagen cross-linking in cardiac hypertrophy.
29377254	9	81	theme	MMP-2	1307:1311	arg1	glycosylation					1268:1280	glycosylation	1268:1280	glycosylation of CD147	1268:1289	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	81	theme	MMP-2	1307:1311	arg1	activity					1295:1302	activity	1295:1302	activity of MMP-2 and MMP-9	1295:1321	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	4	82	theme	left	514:517	arg1	ventricle					519:527	left ventricle	514:527	left ventricle	514:527	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	11	83	theme	activated	1536:1544	arg1	MMPs					1546:1549	the activated MMPs	1532:1549	the activated MMPs	1532:1549	Collagens were degraded by the activated MMPs and then reduced collagen cross-linking.
29377254	0	84	theme	cardiac	64:70	arg1	hypertrophy					72:82	cardiac hypertrophy	64:82	cardiac hypertrophy	64:82	Glycosylated CD147 reduces myocardial collagen cross-linking in cardiac hypertrophy.
29377254	9	85	theme	MMP-9	1317:1321	arg1	glycosylation					1268:1280	glycosylation	1268:1280	glycosylation of CD147	1268:1289	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	9	85	theme	MMP-9	1317:1321	arg1	activity					1295:1302	activity	1295:1302	activity of MMP-2 and MMP-9	1295:1321	On the contrary, tunicamycin, an inhibitor of N-glycan biosynthesis, inhibited glycosylation of CD147 and activity of MMP-2 and MMP-9, and then maintained a stable of collagen cross-linking in the 2K1C rat hearts.
29377254	6	86	theme	N-acetylglucosamine	817:835	arg1	enzyme					866:871	a key enzyme	860:871	a key enzyme for CD147 glycosylation	860:895	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	6	86	theme	N-acetylglucosamine	817:835	arg1	GnT-V					852:856	GnT-V	852:856	GnT-V	852:856	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	6	86	theme	N-acetylglucosamine	817:835	arg1	V					849:849	N-acetylglucosamine transferase V	817:849	N-acetylglucosamine transferase V (GnT-V)	817:857	Ang II can activate N-acetylglucosamine transferase V (GnT-V), a key enzyme for CD147 glycosylation.
29377254	3	87	used	used	401:404	arg2	model					391:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	Goldblatt two-kidney one-clip (2K1C) rat model	350:395	In the present study, Goldblatt two-kidney one-clip (2K1C) rat model was used to simulate Ang II-induced hypertension.
29377254	8	88	from	cross-linking	1103:1115	arg1	ventricle					1143:1151	the hypertrophied left ventricle	1120:1151	the hypertrophied left ventricle	1120:1151	Meanwhile, collagen cross-linking in the hypertrophied left ventricle significantly reduced in 2K1C rats.
29377254	4	89	theme	CD147	606:610	arg1	glycosylation					589:601	glycosylation	589:601	glycosylation	589:601	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
29377254	4	89	theme	CD147	606:610	arg1	expression					574:583	expression	574:583	expression	574:583	The elevated Ang II not only induced the concentric hypertrophy of left ventricle and cardiac fibrosis, but also increased the expression and glycosylation of CD147 in 2K1C rats.
31022500	0	0	theme	naproxen	87:94	arg1	nanoparticles					65:77	crystalline nanoparticles	53:77	crystalline nanoparticles of pure naproxen	53:94	Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
31022500	3	1	from	powders	416:422	arg1	present					397:403	present	397:403	present	397:403	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	5	2	from	freezing	744:751	arg1	LN2					784:786	LN2	784:786	LN2	784:786	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	0	3	theme	pure	82:85	arg1	naproxen					87:94	pure naproxen	82:94	pure naproxen	82:94	Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
31022500	1	4	theme	re-dispersible	143:156	arg1	powders					158:164	re-dispersible powders	143:164	re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose	143:229	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	1	5	theme	hydroxypropyl	251:263	arg1	cellulose					265:273	hydroxypropyl cellulose	251:273	hydroxypropyl cellulose	251:273	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	5	6	dep	does	830:833	arg1	than					825:828	than	825:828	than	825:828	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	3	7	theme	SFD	412:414	arg1	powders					416:422	the SFD powders	408:422	the SFD powders	408:422	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	5	8	theme	large	760:764	arg1	droplets					772:779	the large spray droplets	756:779	the large spray droplets in LN2	756:786	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	5	9	theme	moderately-rapid	727:742	arg1	freezing					744:751	the moderately-rapid freezing	723:751	the moderately-rapid freezing of the large spray droplets in LN2 during SFD	723:797	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	5	10	theme	spray	766:770	arg1	droplets					772:779	the large spray droplets	756:779	the large spray droplets in LN2	756:786	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	3	11	from	present	397:403	arg1	powders					416:422	the SFD powders	408:422	the SFD powders	408:422	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	8	12	theme	dissolved	1171:1179	arg1	fraction					1190:1197	the dissolved naproxen fraction	1167:1197	the dissolved naproxen fraction	1167:1197	After SFD, the dissolved naproxen fraction formed an amorphous solid which re-dissolves on re-hydration whereas the crystalline nanoparticles disaggregate.
31022500	2	13	theme	particle	280:287	arg1	size					289:292	The particle size	276:292	The particle size of the rehydrated powders	276:318	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	4	14	theme	composition	694:704	arg1	nanoparticles					668:680	spray dried nanoparticles	656:680	spray dried nanoparticles of the same composition	656:704	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	5	15	theme	droplets	772:779	arg1	freezing					744:751	the moderately-rapid freezing	723:751	the moderately-rapid freezing of the large spray droplets in LN2 during SFD	723:797	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	7	16	theme	drug	1106:1109	arg1	fraction					1094:1101	a dissolved fraction	1082:1101	a dissolved fraction of drug	1082:1109	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	7	17	theme	media	1141:1145	arg1	milling					1147:1153	media milling	1141:1153	media milling	1141:1153	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	1	18	used	used	127:130	arg2	drying					110:115	Spray freeze drying	97:115	Spray freeze drying (SFD)	97:121	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	1	18	used	used	127:130	arg2	SFD					118:120	SFD	118:120	SFD	118:120	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	5	19	theme	spray	890:894	arg1	drying					896:901	spray drying	890:901	spray drying	890:901	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	0	20	theme	spray	4:8	arg1	freeze-drying					10:22	spray freeze-drying	4:22	spray freeze-drying	4:22	Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
31022500	6	21	theme	original	969:976	arg1	nanodispersion					978:991	the original nanodispersion	965:991	the original nanodispersion	965:991	Re-dispersion was also found to depend strongly on the pH of the original nanodispersion.
31022500	7	22	theme	dissolved	1084:1092	arg1	fraction					1094:1101	a dissolved fraction	1082:1101	a dissolved fraction of drug	1082:1109	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	1	23	theme	crystalline	169:179	arg1	nanodispersions					192:206	crystalline, pure-drug nanodispersions	169:206	crystalline, pure-drug nanodispersions of naproxen	169:218	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	5	24	theme	evaporative	835:845	arg1	drying					847:852	evaporative drying	835:852	evaporative drying of the much smaller droplets during spray drying	835:901	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	5	25	from	LN2	784:786	arg1	freezing					744:751	the moderately-rapid freezing	723:751	the moderately-rapid freezing of the large spray droplets in LN2 during SFD	723:797	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	1	26	theme	Spray	97:101	arg1	SFD					118:120	SFD	118:120	SFD	118:120	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	1	26	theme	Spray	97:101	arg1	drying					110:115	Spray freeze drying	97:115	Spray freeze drying (SFD)	97:121	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	4	27	theme	same	689:692	arg1	composition					694:704	the same composition	685:704	the same composition	685:704	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	6	28	theme	nanodispersion	978:991	arg1	pH					959:960	the pH	955:960	the pH of the original nanodispersion	955:991	Re-dispersion was also found to depend strongly on the pH of the original nanodispersion.
31022500	1	29	theme	freeze	103:108	arg1	SFD					118:120	SFD	118:120	SFD	118:120	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	1	29	theme	freeze	103:108	arg1	drying					110:115	Spray freeze drying	97:115	Spray freeze drying (SFD)	97:121	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	4	30	theme	spray	656:660	arg1	nanoparticles					668:680	spray dried nanoparticles	656:680	spray dried nanoparticles of the same composition	656:704	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	2	31	theme	powders	312:318	arg1	size					289:292	The particle size	276:292	The particle size of the rehydrated powders	276:318	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	4	32	theme	nanoparticles	590:602	arg1	disaggregation					564:577	The disaggregation	560:577	The disaggregation of the SFD nanoparticles	560:602	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	4	32	theme	nanoparticles	590:602	arg1	superior					608:615	superior	608:615	superior	608:615	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	3	33	with	re-dispersion	466:478	arg1	with					511:514	with	511:514	with	511:514	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	3	33	with	re-dispersion	466:478	arg1	stirring					495:502	stirring	495:502	stirring	495:502	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	3	33	with	re-dispersion	466:478	arg1	water					485:489	water	485:489	water	485:489	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	2	34	theme	scattering/Mie	354:367	arg1	analysis					369:376	static light scattering/Mie analysis	341:376	static light scattering/Mie analysis	341:376	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	1	35	theme	nanodispersions	192:206	arg1	powders					158:164	re-dispersible powders	143:164	re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose	143:229	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	4	36	theme	SFD	586:588	arg1	nanoparticles					590:602	the SFD nanoparticles	582:602	the SFD nanoparticles	582:602	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	5	37	from	droplets	772:779	arg1	LN2					784:786	LN2	784:786	LN2	784:786	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	1	38	dep	crystalline	169:179	arg1	pure-drug					182:190	pure-drug	182:190	pure-drug	182:190	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	5	39	theme	smaller	866:872	arg1	droplets					874:881	the much smaller droplets	857:881	the much smaller droplets	857:881	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	5	40	theme	droplets	874:881	arg1	drying					847:852	evaporative drying	835:852	evaporative drying of the much smaller droplets during spray drying	835:901	It appears that the moderately-rapid freezing of the large spray droplets in LN2 during SFD produces less aggregation than does evaporative drying of the much smaller droplets during spray drying.
31022500	1	41	theme	naproxen	211:218	arg1	nanodispersions					192:206	crystalline, pure-drug nanodispersions	169:206	crystalline, pure-drug nanodispersions of naproxen	169:218	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	0	42	theme	crystalline	53:63	arg1	nanoparticles					65:77	crystalline nanoparticles	53:77	crystalline nanoparticles of pure naproxen	53:94	Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
31022500	2	43	theme	light	348:352	arg1	analysis					369:376	static light scattering/Mie analysis	341:376	static light scattering/Mie analysis	341:376	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	7	44	theme	acid	1022:1025	arg1	solubility					998:1007	The solubility	994:1007	The solubility of this weak acid	994:1025	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	7	44	theme	acid	1022:1025	arg1	greater					1030:1036	greater	1030:1036	greater	1030:1036	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	2	45	theme	static	341:346	arg1	analysis					369:376	static light scattering/Mie analysis	341:376	static light scattering/Mie analysis	341:376	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	3	46	dep	water	485:489	arg1	either					504:509	either	504:509	either	504:509	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	7	47	theme	higher	1041:1046	arg1	pH					1048:1049	higher pH	1041:1049	higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling	1041:1153	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	2	48	theme	rehydrated	301:310	arg1	powders					312:318	the rehydrated powders	297:318	the rehydrated powders	297:318	The particle size of the rehydrated powders was determined using static light scattering/Mie analysis.
31022500	3	49	attach	present	397:403	arg2	nanoparticles					383:395	The nanoparticles	379:395	The nanoparticles present in the SFD powders	379:422	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	3	49	attach	present	397:403	arg1	powders					416:422	the SFD powders	408:422	the SFD powders	408:422	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	8	50	theme	naproxen	1181:1188	arg1	fraction					1190:1197	the dissolved naproxen fraction	1167:1197	the dissolved naproxen fraction	1167:1197	After SFD, the dissolved naproxen fraction formed an amorphous solid which re-dissolves on re-hydration whereas the crystalline nanoparticles disaggregate.
31022500	7	51	theme	fraction	1094:1101	arg1	formation					1069:1077	formation	1069:1077	formation of a dissolved fraction of drug	1069:1109	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	4	52	theme	dried	662:666	arg1	nanoparticles					668:680	spray dried nanoparticles	656:680	spray dried nanoparticles of the same composition	656:704	The disaggregation of the SFD nanoparticles was superior to that reported in the literature for spray dried nanoparticles of the same composition.
31022500	7	53	theme	weak	1017:1020	arg1	acid					1022:1025	this weak acid	1012:1025	this weak acid	1012:1025	The solubility of this weak acid is greater at higher pH which resulted in formation of a dissolved fraction of drug in the nanodispersions during media milling.
31022500	3	54	theme	additional	527:536	arg1	treatment					549:557	additional ultrasonic treatment	527:557	additional ultrasonic treatment	527:557	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	0	55	theme	nanoparticles	65:77	arg1	re-dispersion					36:48	the re-dispersion	32:48	the re-dispersion of crystalline nanoparticles of pure naproxen	32:94	Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
31022500	3	56	theme	present	397:403	arg1	nanoparticles					383:395	The nanoparticles	379:395	The nanoparticles present in the SFD powders	379:422	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31022500	1	57	from	powders	158:164	arg1	lactose					223:229	lactose	223:229	lactose	223:229	Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose.
31022500	3	58	theme	ultrasonic	538:547	arg1	treatment					549:557	additional ultrasonic treatment	527:557	additional ultrasonic treatment	527:557	The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment.
31760002	0	0	theme	complex	82:88	arg1	properties					42:51	the properties	38:51	the properties of potato starch-lauric acid complex and potato starch-based film	38:117	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	6	1	theme	vapour	1298:1303	arg1	permeability					1305:1316	water vapour permeability	1292:1316	water vapour permeability	1292:1316	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	1	2	theme	time	157:160	arg1	effects					124:130	The effects	120:130	The effects of different debranching time on the properties of potato starch and its films	120:209	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	0	3	theme	acid	77:80	arg1	complex					82:88	potato starch-lauric acid complex	56:88	potato starch-lauric acid complex	56:88	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	6	4	theme	water	1292:1296	arg1	permeability					1305:1316	water vapour permeability	1292:1316	water vapour permeability	1292:1316	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	4	5	theme	higher	703:708	arg1	content					722:728	higher lauric acid content	703:728	higher lauric acid content	703:728	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	0	6	theme	potato	94:99	arg1	film					114:117	potato starch-based film	94:117	potato starch-based film	94:117	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	0	7	from	Effects	0:6	arg1	properties					42:51	the properties	38:51	the properties of potato starch-lauric acid complex and potato starch-based film	38:117	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	6	8	theme	lowest	1285:1290	arg1	permeability					1305:1316	water vapour permeability	1292:1316	water vapour permeability	1292:1316	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	6	9	theme	debranching	1230:1240	arg1	treatment					1242:1250	the debranching treatment	1226:1250	the debranching treatment	1226:1250	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	10	theme	debranching	515:525	arg1	treatment					527:535	the shorter debranching treatment	503:535	the shorter debranching treatment	503:535	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	4	11	theme	untreated	747:755	arg1	sample					757:762	the untreated sample	743:762	the untreated sample	743:762	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	5	12	theme	relative	969:976	arg1	crystallinity					978:990	relative crystallinity	969:990	relative crystallinity	969:990	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	4	13	theme	lauric	710:715	arg1	content					722:728	higher lauric acid content	703:728	higher lauric acid content	703:728	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	0	14	theme	film	114:117	arg1	properties					42:51	the properties	38:51	the properties of potato starch-lauric acid complex and potato starch-based film	38:117	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	6	15	theme	debranching	1092:1102	arg1	treatment					1104:1112	the pullulanase debranching treatment	1076:1112	the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1076:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	4	16	theme	starch-lipid	673:684	arg1	complexes					686:694	The debranched starch-lipid complexes	658:694	The debranched starch-lipid complexes	658:694	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	6	17	theme	electron	1134:1141	arg1	microscope					1143:1152	1.5 h. Scanning electron microscope	1118:1152	1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1118:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	0	18	theme	starch-based	101:112	arg1	film					114:117	potato starch-based film	94:117	potato starch-based film	94:117	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	1	19	from	effects	124:130	arg1	properties					169:178	the properties	165:178	the properties of potato starch and its films	165:209	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	3	20	theme	shorter	507:513	arg1	treatment					527:535	the shorter debranching treatment	503:535	the shorter debranching treatment	503:535	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	21	theme	pullulanase	1080:1090	arg1	treatment					1104:1112	the pullulanase debranching treatment	1076:1112	the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1076:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	22	theme	treatment	353:361	arg1	effects					318:324	The effects	314:324	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin	314:410	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	23	theme	high	1043:1046	arg1	enthalpy					1056:1063	a high melting enthalpy	1041:1063	a high melting enthalpy (ΔH)	1041:1068	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	6	23	theme	high	1043:1046	arg1	ΔH					1066:1067	ΔH	1066:1067	ΔH	1066:1067	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	24	theme	linear	552:557	arg1	chains					559:564	more linear chains	547:564	more linear chains	547:564	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	25	theme	amylopectin	400:410	arg1	distributions					383:395	the chain-length distributions	366:395	the chain-length distributions of amylopectin	366:410	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	5	26	theme	complexes	999:1007	arg1	intensity					955:963	diffraction intensity	943:963	diffraction intensity	943:963	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	5	26	theme	complexes	999:1007	arg1	crystallinity					978:990	relative crystallinity	969:990	relative crystallinity	969:990	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	1	27	theme	potato	183:188	arg1	starch					190:195	potato starch	183:195	potato starch	183:195	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	3	28	theme	pullulanase	329:339	arg1	treatment					353:361	pullulanase debranching treatment	329:361	pullulanase debranching treatment	329:361	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	29	theme	treatment	1357:1365	arg1	time					1367:1370	the treatment time	1353:1370	the treatment time for 1.5 h	1353:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	1	30	dep	different	135:143	arg1	debranching					145:155	debranching	145:155	debranching	145:155	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	1	31	theme	starch	190:195	arg1	properties					169:178	the properties	165:178	the properties of potato starch and its films	165:209	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	3	32	theme	debranching	341:351	arg1	treatment					353:361	pullulanase debranching treatment	329:361	pullulanase debranching treatment	329:361	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	33	theme	composite	1193:1201	arg1	film					1203:1206	the composite film	1189:1206	the composite film	1189:1206	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	34	theme	acid	642:645	arg1	complexes					647:655	potato starch-lauric acid complexes	621:655	potato starch-lauric acid complexes	621:655	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	35	theme	more	547:550	arg1	chains					559:564	more linear chains	547:564	more linear chains	547:564	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	36	theme	exchange	452:459	arg1	HPAEC					477:481	HPAEC	477:481	HPAEC	477:481	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	36	theme	exchange	452:459	arg1	chromatography					461:474	high performance anion exchange chromatography	429:474	high performance anion exchange chromatography (HPAEC)	429:482	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	4	37	theme	acid	717:720	arg1	content					722:728	higher lauric acid content	703:728	higher lauric acid content	703:728	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	3	38	theme	complexes	647:655	arg1	formation					608:616	the formation	604:616	the formation of potato starch-lauric acid complexes	604:655	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	2	39	theme	Potato	227:232	arg1	granules					241:248	Potato starch granules	227:248	Potato starch granules	227:248	Potato starch granules were debranched with pullulanase for 0, 0.5, 1.0, 1.5 or 2.0 h.
31760002	4	40	theme	debranched	662:671	arg1	complexes					686:694	The debranched starch-lipid complexes	658:694	The debranched starch-lipid complexes	658:694	The debranched starch-lipid complexes showed higher lauric acid content than that of the untreated sample.
31760002	3	41	theme	high	429:432	arg1	HPAEC					477:481	HPAEC	477:481	HPAEC	477:481	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	41	theme	high	429:432	arg1	chromatography					461:474	high performance anion exchange chromatography	429:474	high performance anion exchange chromatography (HPAEC)	429:482	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	0	42	theme	pullulanase	11:21	arg1	debranching					23:33	pullulanase debranching	11:33	pullulanase debranching	11:33	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	5	43	theme	undebranched	876:887	arg1	sample					889:894	the undebranched sample	872:894	the undebranched sample	872:894	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	3	44	theme	potato	621:626	arg1	complexes					647:655	potato starch-lauric acid complexes	621:655	potato starch-lauric acid complexes	621:655	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	0	45	theme	debranching	23:33	arg1	Effects					0:6	Effects	0:6	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.	0:118	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	3	46	theme	performance	434:444	arg1	HPAEC					477:481	HPAEC	477:481	HPAEC	477:481	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	46	theme	performance	434:444	arg1	chromatography					461:474	high performance anion exchange chromatography	429:474	high performance anion exchange chromatography (HPAEC)	429:482	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	5	47	theme	diffraction	799:809	arg1	intensity					811:819	the diffraction intensity	795:819	the diffraction intensity of the debranched sample	795:844	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	5	47	theme	diffraction	799:809	arg1	stronger					850:857	stronger	850:857	stronger	850:857	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	6	48	theme	tensile	1330:1336	arg1	strength					1338:1345	highest tensile strength	1322:1345	highest tensile strength	1322:1345	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	49	theme	starch-lauric	628:640	arg1	complexes					647:655	potato starch-lauric acid complexes	621:655	potato starch-lauric acid complexes	621:655	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	5	50	theme	debranched	828:837	arg1	sample					839:844	the debranched sample	824:844	the debranched sample	824:844	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	5	51	theme	debranching	910:920	arg1	time					922:925	the debranching time	906:925	the debranching time	906:925	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	3	52	theme	chain-length	370:381	arg1	distributions					383:395	the chain-length distributions	366:395	the chain-length distributions of amylopectin	366:410	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	53	theme	highest	1322:1328	arg1	strength					1338:1345	highest tensile strength	1322:1345	highest tensile strength	1322:1345	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	54	theme	favourable	571:580	arg1	lengths					582:588	favourable lengths	571:588	favourable lengths	571:588	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	55	from	effects	318:324	arg1	distributions					383:395	the chain-length distributions	366:395	the chain-length distributions of amylopectin	366:410	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	6	56	theme	film	1203:1206	arg1	flatter					1212:1218	flatter	1212:1218	flatter	1212:1218	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	6	56	theme	film	1203:1206	arg1	surface					1178:1184	the surface	1174:1184	the surface of the composite film	1174:1206	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	5	57	theme	X-ray	765:769	arg1	diffraction					771:781	X-ray diffraction	765:781	X-ray diffraction	765:781	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	6	58	theme	Scanning	1125:1132	arg1	microscope					1143:1152	1.5 h. Scanning electron microscope	1118:1152	1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1118:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	5	59	theme	diffraction	943:953	arg1	intensity					955:963	diffraction intensity	943:963	diffraction intensity	943:963	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	5	60	theme	sample	839:844	arg1	intensity					811:819	the diffraction intensity	795:819	the diffraction intensity of the debranched sample	795:844	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	5	60	theme	sample	839:844	arg1	stronger					850:857	stronger	850:857	stronger	850:857	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	6	61	theme	1.5 h.	1118:1123	arg1	microscope					1143:1152	1.5 h. Scanning electron microscope	1118:1152	1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1118:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	0	62	theme	starch-lauric	63:75	arg1	complex					82:88	potato starch-lauric acid complex	56:88	potato starch-lauric acid complex	56:88	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	1	63	theme	films	205:209	arg1	properties					169:178	the properties	165:178	the properties of potato starch and its films	165:209	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	6	64	dep	microscope	1143:1152	arg1	indicated					1159:1167	indicated	1159:1167	indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h	1159:1380	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	3	65	theme	anion	446:450	arg1	HPAEC					477:481	HPAEC	477:481	HPAEC	477:481	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	3	65	theme	anion	446:450	arg1	chromatography					461:474	high performance anion exchange chromatography	429:474	high performance anion exchange chromatography (HPAEC)	429:482	The effects of pullulanase debranching treatment on the chain-length distributions of amylopectin were analysed by high performance anion exchange chromatography (HPAEC), which proved that the shorter debranching treatment generated more linear chains with favourable lengths to facilitate the formation of potato starch-lauric acid complexes.
31760002	1	66	theme	different	135:143	arg1	time					157:160	different debranching time	135:160	different debranching time	135:160	The effects of different debranching time on the properties of potato starch and its films were evaluated.
31760002	0	67	theme	potato	56:61	arg1	complex					82:88	potato starch-lauric acid complex	56:88	potato starch-lauric acid complex	56:88	Effects of pullulanase debranching on the properties of potato starch-lauric acid complex and potato starch-based film.
31760002	2	68	theme	starch	234:239	arg1	granules					241:248	Potato starch granules	227:248	Potato starch granules	227:248	Potato starch granules were debranched with pullulanase for 0, 0.5, 1.0, 1.5 or 2.0 h.
31760002	5	69	dep	intensity	955:963	arg1	the					939:941	the	939:941	the	939:941	X-ray diffraction showed that the diffraction intensity of the debranched sample was stronger than that of the undebranched sample, and when the debranching time was >1.5 h, the diffraction intensity and relative crystallinity of the complexes decreased.
31760002	6	70	theme	melting	1048:1054	arg1	enthalpy					1056:1063	a high melting enthalpy	1041:1063	a high melting enthalpy (ΔH)	1041:1068	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
31760002	6	70	theme	melting	1048:1054	arg1	ΔH					1066:1067	ΔH	1066:1067	ΔH	1066:1067	The sample exhibited a high melting enthalpy (ΔH) under the pullulanase debranching treatment for 1.5 h. Scanning electron microscope data indicated that the surface of the composite film was flatter after the debranching treatment and that the films exhibited the lowest water vapour permeability and highest tensile strength after the treatment time for 1.5 h.
30342153	0	0	theme	diameter	81:88	arg1	distribution					90:101	fiber diameter distribution	75:101	fiber diameter distribution	75:101	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	1	1	theme	CNFs	340:343	arg1	dispersions					312:322	dispersions	312:322	dispersions of the prepared CNFs	312:343	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	6	2	theme	initial	1259:1265	arg1	modulus					1267:1273	the initial modulus	1255:1273	the initial modulus	1255:1273	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	4	3	theme	mechanical	988:997	arg1	analysis					999:1006	dynamic mechanical analysis	980:1006	dynamic mechanical analysis	980:1006	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	0	4	theme	fiber	75:79	arg1	distribution					90:101	fiber diameter distribution	75:101	fiber diameter distribution	75:101	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	5	5	theme	mechanical	1085:1094	arg1	properties					1096:1105	these characteristic mechanical properties	1064:1105	these characteristic mechanical properties of the NR-CNFs	1064:1120	It was concluded that these characteristic mechanical properties of the NR-CNFs were determined by the morphologies of the CNFs.
30342153	1	6	theme	diameter	433:440	arg1	distributions					442:454	the diameter distributions	429:454	the diameter distributions of the CNFs	429:466	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	7	theme	natural	556:562	arg1	NR					572:573	NR	572:573	NR	572:573	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	7	theme	natural	556:562	arg1	rubber					564:569	natural rubber	556:569	natural rubber (NR)	556:574	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	8	dep	d	612:612	arg1	0.1-10 μm					615:623	0.1-10 μm	615:623	0.1-10 μm	615:623	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	1	9	theme	kraft	273:277	arg1	pulp					279:282	kraft pulp	273:282	kraft pulp using wet disk milling	273:305	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	7	10	theme	diameter	1461:1468	arg1	distribution					1470:1481	their diameter distribution	1455:1481	their diameter distribution	1455:1481	Understanding the effect of their diameter distribution can potentially reduce the production cost of CNFs and thus expand their applicability.
30342153	6	11	theme	tensile	1364:1370	arg1	strength					1372:1379	the tensile strength	1360:1379	the tensile strength without compromising viscoelastic properties	1360:1424	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	1	12	theme	pulp	279:282	arg1	fibrillation					257:268	the mechanical fibrillation	242:268	the mechanical fibrillation of kraft pulp using wet disk milling	242:305	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	1	13	with	nanofibers	169:178	arg1	degrees					202:208	different degrees	192:208	different degrees of fibrillation	192:224	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	6	14	theme	high	1235:1238	arg1	improvement					1240:1250	high improvement	1235:1250	high improvement of the initial modulus	1235:1273	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	7	15	theme	distribution	1470:1481	arg1	effect					1445:1450	the effect	1441:1450	the effect of their diameter distribution	1441:1481	Understanding the effect of their diameter distribution can potentially reduce the production cost of CNFs and thus expand their applicability.
30342153	3	16	theme	tensile	757:763	arg1	strength					765:772	the tensile strength	753:772	the tensile strength	753:772	The high-fibrillated CNFs (d: <0.1 μm) enhanced the tensile strength even further while maintaining the elongation at break.
30342153	6	17	theme	high-fibrillated	1306:1321	arg1	CNFs					1323:1326	the high-fibrillated CNFs	1302:1326	the high-fibrillated CNFs	1302:1326	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	1	18	theme	differential	363:374	arg1	DCS					403:405	DCS	403:405	DCS	403:405	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	1	18	theme	differential	363:374	arg1	sedimentation					388:400	differential centrifugal sedimentation	363:400	differential centrifugal sedimentation (DCS)	363:406	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	19	theme	medium-fibrillated	587:604	arg1	CNFs					606:609	the medium-fibrillated CNFs	583:609	the medium-fibrillated CNFs (d: 0.1-10 μm)	583:624	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	4	20	theme	scanning	942:949	arg1	microscopy					960:969	field-emission scanning electron microscopy	927:969	field-emission scanning electron microscopy imaging	927:977	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	1	21	theme	centrifugal	376:386	arg1	DCS					403:405	DCS	403:405	DCS	403:405	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	1	21	theme	centrifugal	376:386	arg1	sedimentation					388:400	differential centrifugal sedimentation	363:400	differential centrifugal sedimentation (DCS)	363:406	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	4	22	theme	field-emission	927:940	arg1	microscopy					960:969	field-emission scanning electron microscopy	927:969	field-emission scanning electron microscopy imaging	927:977	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	1	23	theme	CNFs	463:466	arg1	distributions					442:454	the diameter distributions	429:454	the diameter distributions of the CNFs	429:466	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	24	contain	had	523:525	arg1	CNFs					489:492	The low-fibrillated CNFs	469:492	The low-fibrillated CNFs (fiber diameter (d): >10 μm)	469:521	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	24	contain	had	523:525	arg2	effect					546:551	a weak reinforcing effect	527:551	a weak reinforcing effect	527:551	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	5	25	theme	NR-CNFs	1114:1120	arg1	properties					1096:1105	these characteristic mechanical properties	1064:1105	these characteristic mechanical properties of the NR-CNFs	1064:1120	It was concluded that these characteristic mechanical properties of the NR-CNFs were determined by the morphologies of the CNFs.
30342153	3	26	dep	d	732:732	arg1	<0.1 μm					735:741	<0.1 μm	735:741	d: <0.1 μm	732:741	The high-fibrillated CNFs (d: <0.1 μm) enhanced the tensile strength even further while maintaining the elongation at break.
30342153	6	27	theme	medium-fibrillated	1202:1219	arg1	CNFs					1221:1224	the medium-fibrillated CNFs	1198:1224	the medium-fibrillated CNFs	1198:1224	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	3	28	dep	CNFs	726:729	arg1	d					732:732	d	732:732	d: <0.1 μm	732:741	The high-fibrillated CNFs (d: <0.1 μm) enhanced the tensile strength even further while maintaining the elongation at break.
30342153	1	29	theme	different	192:200	arg1	degrees					202:208	different degrees	192:208	different degrees of fibrillation	192:224	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	4	30	theme	microscopy	960:969	arg1	imaging					971:977	field-emission scanning electron microscopy imaging	927:977	field-emission scanning electron microscopy imaging	927:977	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	1	31	theme	wet	290:292	arg1	milling					299:305	wet disk milling	290:305	wet disk milling	290:305	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	0	32	theme	rubber	22:27	arg1	Properties					0:9	Properties	0:9	Properties of natural rubber	0:27	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	1	33	theme	disk	294:297	arg1	milling					299:305	wet disk milling	290:305	wet disk milling	290:305	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	0	34	theme	centrifugal	132:142	arg1	sedimentation					144:156	differential centrifugal sedimentation	119:156	differential centrifugal sedimentation	119:156	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	2	35	dep	diameter	501:508	arg1	>10 μm					515:520	>10 μm	515:520	fiber diameter (d): >10 μm	495:520	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	0	36	theme	natural	14:20	arg1	rubber					22:27	natural rubber	14:27	natural rubber	14:27	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	5	37	theme	characteristic	1070:1083	arg1	properties					1096:1105	these characteristic mechanical properties	1064:1105	these characteristic mechanical properties of the NR-CNFs	1064:1120	It was concluded that these characteristic mechanical properties of the NR-CNFs were determined by the morphologies of the CNFs.
30342153	0	38	theme	differential	119:130	arg1	sedimentation					144:156	differential centrifugal sedimentation	119:156	differential centrifugal sedimentation	119:156	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	7	39	theme	CNFs	1529:1532	arg1	cost					1521:1524	the production cost	1506:1524	the production cost of CNFs	1506:1532	Understanding the effect of their diameter distribution can potentially reduce the production cost of CNFs and thus expand their applicability.
30342153	6	40	theme	modulus	1267:1273	arg1	improvement					1240:1250	high improvement	1235:1250	high improvement of the initial modulus	1235:1273	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	1	41	theme	fibrillation	213:224	arg1	degrees					202:208	different degrees	192:208	different degrees of fibrillation	192:224	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	42	theme	low-fibrillated	473:487	arg1	CNFs					489:492	The low-fibrillated CNFs	469:492	The low-fibrillated CNFs (fiber diameter (d): >10 μm)	469:521	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	43	from	break	698:702	arg1	elongation					684:693	the elongation	680:693	the elongation at break	680:702	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	6	44	theme	strength	1372:1379	arg1	enhancement					1345:1355	enhancement	1345:1355	enhancement of the tensile strength without compromising viscoelastic properties	1345:1424	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	4	45	theme	composites	866:875	arg1	mechanism					846:854	The reinforcing mechanism	830:854	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs)	830:908	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	4	46	theme	dynamic	980:986	arg1	analysis					999:1006	dynamic mechanical analysis	980:1006	dynamic mechanical analysis	980:1006	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	4	47	theme	electron	951:958	arg1	microscopy					960:969	field-emission scanning electron microscopy	927:969	field-emission scanning electron microscopy imaging	927:977	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	4	48	theme	reinforcing	834:844	arg1	mechanism					846:854	The reinforcing mechanism	830:854	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs)	830:908	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	6	49	theme	branching	1175:1183	arg1	structure					1185:1193	The branching structure	1171:1193	The branching structure of the medium-fibrillated CNFs	1171:1224	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	2	50	theme	initial	651:657	arg1	modulus					659:665	the initial modulus	647:665	the initial modulus	647:665	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	0	51	theme	cellulose	45:53	arg1	nanofibers					55:64	cellulose nanofibers	45:64	cellulose nanofibers based on fiber diameter distribution	45:101	Properties of natural rubber reinforced with cellulose nanofibers based on fiber diameter distribution as estimated by differential centrifugal sedimentation.
30342153	3	52	theme	high-fibrillated	709:724	arg1	CNFs					726:729	The high-fibrillated CNFs	705:729	The high-fibrillated CNFs (d: <0.1 μm)	705:742	The high-fibrillated CNFs (d: <0.1 μm) enhanced the tensile strength even further while maintaining the elongation at break.
30342153	2	53	dep	CNFs	489:492	arg1	d					511:511	d	511:511	d	511:511	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	53	dep	CNFs	489:492	arg1	diameter					501:508	fiber diameter	495:508	fiber diameter (d): >10 μm	495:520	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	6	54	theme	CNFs	1221:1224	arg1	structure					1185:1193	The branching structure	1171:1193	The branching structure of the medium-fibrillated CNFs	1171:1224	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	4	55	theme	uptake	1021:1026	arg1	measurements					1028:1039	toluene uptake measurements	1013:1039	toluene uptake measurements	1013:1039	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	2	56	theme	reinforcing	534:544	arg1	effect					546:551	a weak reinforcing effect	527:551	a weak reinforcing effect	527:551	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	7	57	theme	production	1510:1519	arg1	cost					1521:1524	the production cost	1506:1524	the production cost of CNFs	1506:1532	Understanding the effect of their diameter distribution can potentially reduce the production cost of CNFs and thus expand their applicability.
30342153	5	58	theme	CNFs	1165:1168	arg1	morphologies					1145:1156	the morphologies	1141:1156	the morphologies of the CNFs	1141:1168	It was concluded that these characteristic mechanical properties of the NR-CNFs were determined by the morphologies of the CNFs.
30342153	4	59	theme	toluene	1013:1019	arg1	measurements					1028:1039	toluene uptake measurements	1013:1039	toluene uptake measurements	1013:1039	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	6	60	theme	viscoelastic	1402:1413	arg1	properties					1415:1424	viscoelastic properties	1402:1424	viscoelastic properties	1402:1424	The branching structure of the medium-fibrillated CNFs affected high improvement of the initial modulus, and the network formed by the high-fibrillated CNFs were involved in enhancement of the tensile strength without compromising viscoelastic properties.
30342153	2	61	theme	weak	529:532	arg1	effect					546:551	a weak reinforcing effect	527:551	a weak reinforcing effect	527:551	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	4	62	theme	NR	863:864	arg1	composites					866:875	the NR composites	859:875	the NR composites reinforced by the CNFs (NR-CNFs)	859:908	The reinforcing mechanism of the NR composites reinforced by the CNFs (NR-CNFs) was confirmed by field-emission scanning electron microscopy imaging, dynamic mechanical analysis, and toluene uptake measurements.
30342153	1	63	theme	Cellulose	159:167	arg1	nanofibers					169:178	Cellulose nanofibers	159:178	Cellulose nanofibers (CNFs) with different degrees of fibrillation	159:224	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	1	63	theme	Cellulose	159:167	arg1	CNFs					181:184	CNFs	181:184	CNFs	181:184	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	1	64	theme	mechanical	246:255	arg1	fibrillation					257:268	the mechanical fibrillation	242:268	the mechanical fibrillation of kraft pulp using wet disk milling	242:305	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	65	theme	fiber	495:499	arg1	d					511:511	d	511:511	d	511:511	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	2	65	theme	fiber	495:499	arg1	diameter					501:508	fiber diameter	495:508	fiber diameter (d): >10 μm	495:520	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30342153	1	66	theme	prepared	331:338	arg1	CNFs					340:343	the prepared CNFs	327:343	the prepared CNFs	327:343	Cellulose nanofibers (CNFs) with different degrees of fibrillation are prepared by the mechanical fibrillation of kraft pulp using wet disk milling, and dispersions of the prepared CNFs were subjected to differential centrifugal sedimentation (DCS) in order to estimate the diameter distributions of the CNFs.
30342153	2	67	dep	CNFs	606:609	arg1	d					612:612	d	612:612	d: 0.1-10 μm	612:623	The low-fibrillated CNFs (fiber diameter (d): >10 μm) had a weak reinforcing effect on natural rubber (NR), while the medium-fibrillated CNFs (d: 0.1-10 μm) dramatically improve the initial modulus and decrease the elongation at break.
30341312	5	0	theme	MCC	687:689	arg1	defibrillation					669:682	An enhanced defibrillation	657:682	An enhanced defibrillation of MCC	657:689	An enhanced defibrillation of MCC has been observed following the application of SLP.
30341312	7	1	theme	better	1076:1081	arg1	stability					1091:1099	better thermal stability	1076:1099	better thermal stability	1076:1099	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	3	2	theme	ultrasonication	462:476	arg1	treatments					478:487	ultrasonication treatments	462:487	ultrasonication treatments	462:487	In this work we demonstrate the modification of microcrystalline cellulose (MCC) by applying the SLP combined with ultrasonication treatments.
30341312	7	3	theme	thermal	1083:1089	arg1	stability					1091:1099	better thermal stability	1076:1099	better thermal stability	1076:1099	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	0	4	from	Cellulose	0:8	arg1	treatment					67:75	liquid treatment	60:75	liquid treatment	60:75	Cellulose defibrillation and functionalization by plasma in liquid treatment.
30341312	6	5	dep	Fourier	793:799	arg1	transform					801:809	transform	801:809	transform infrared spectroscopy	801:831	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	8	6	theme	reinforcing	1319:1329	arg1	ability					1331:1337	an improved reinforcing ability	1307:1337	an improved reinforcing ability of biopolymers	1307:1352	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	4	7	theme	water	641:645	arg1	mixtures					647:654	acetonitrile:water mixtures	628:654	acetonitrile:water mixtures	628:654	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	4	8	theme	reactive	541:548	arg1	gas					584:586	an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas	521:586	an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas	521:586	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	2	9	theme	environmental-friendly	296:317	arg1	potential					268:276	great potential	262:276	great potential	262:276	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	2	9	theme	environmental-friendly	296:317	arg1	method					319:324	a low-cost and environmental-friendly method	281:324	a low-cost and environmental-friendly method to modify cellulose	281:344	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	7	10	theme	modified	1043:1050	arg1	fibers					1062:1067	the plasma modified cellulose fibers	1032:1067	the plasma modified cellulose fibers	1032:1067	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	1	11	theme	Submerged	78:86	arg1	method					131:136	a new and promising method	111:136	a new and promising method to modify powder materials	111:163	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	11	theme	Submerged	78:86	arg1	SLP					103:105	SLP	103:105	SLP	103:105	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	11	theme	Submerged	78:86	arg1	plasma					95:100	Submerged liquid plasma	78:100	Submerged liquid plasma (SLP)	78:106	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	8	12	theme	liquid	1161:1166	arg1	method					1186:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	1	13	theme	powder	148:153	arg1	materials					155:163	powder materials	148:163	powder materials	148:163	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	5	14	theme	enhanced	660:667	arg1	defibrillation					669:682	An enhanced defibrillation	657:682	An enhanced defibrillation of MCC	657:689	An enhanced defibrillation of MCC has been observed following the application of SLP.
30341312	4	15	dep	inert	524:528	arg1	argon					531:535	argon	531:535	argon	531:535	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	7	16	theme	cellulose	1052:1060	arg1	fibers					1062:1067	the plasma modified cellulose fibers	1032:1067	the plasma modified cellulose fibers	1032:1067	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	1	17	theme	liquid	88:93	arg1	method					131:136	a new and promising method	111:136	a new and promising method to modify powder materials	111:163	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	17	theme	liquid	88:93	arg1	SLP					103:105	SLP	103:105	SLP	103:105	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	17	theme	liquid	88:93	arg1	plasma					95:100	Submerged liquid plasma	78:100	Submerged liquid plasma (SLP)	78:106	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	8	18	theme	unique	1202:1207	arg1	approach					1209:1216	a unique approach	1200:1216	a unique approach for the activation of cellulose for defibrillation and functionalization	1200:1289	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	8	19	theme	submerged	1151:1159	arg1	method					1186:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	6	20	theme	nitrogen	918:925	arg1	moieties					927:934	oxygen or nitrogen moieties	908:934	oxygen or nitrogen moieties	908:934	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	6	21	with	functionalization	878:894	arg1	moieties					927:934	oxygen or nitrogen moieties	908:934	oxygen or nitrogen moieties	908:934	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	4	22	theme	inert	524:528	arg1	gas					584:586	an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas	521:586	an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas	521:586	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	7	23	theme	plasma	1036:1041	arg1	fibers					1062:1067	the plasma modified cellulose fibers	1032:1067	the plasma modified cellulose fibers	1032:1067	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	3	24	theme	microcrystalline	395:410	arg1	cellulose					412:420	microcrystalline cellulose	395:420	microcrystalline cellulose (MCC)	395:426	In this work we demonstrate the modification of microcrystalline cellulose (MCC) by applying the SLP combined with ultrasonication treatments.
30341312	3	24	theme	microcrystalline	395:410	arg1	MCC					423:425	MCC	423:425	MCC	423:425	In this work we demonstrate the modification of microcrystalline cellulose (MCC) by applying the SLP combined with ultrasonication treatments.
30341312	5	25	theme	SLP	738:740	arg1	application					723:733	the application	719:733	the application of SLP	719:740	An enhanced defibrillation of MCC has been observed following the application of SLP.
30341312	2	26	theme	great	262:266	arg1	potential					268:276	great potential	262:276	great potential	262:276	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	2	26	theme	great	262:266	arg1	method					319:324	a low-cost and environmental-friendly method	281:324	a low-cost and environmental-friendly method to modify cellulose	281:344	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	7	27	theme	pristine	1132:1139	arg1	PHB					1141:1143	pristine PHB	1132:1143	pristine PHB	1132:1143	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	6	28	theme	oxygen	908:913	arg1	moieties					927:934	oxygen or nitrogen moieties	908:934	oxygen or nitrogen moieties	908:934	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	8	29	theme	biopolymers	1342:1352	arg1	ability					1331:1337	an improved reinforcing ability	1307:1337	an improved reinforcing ability of biopolymers	1307:1352	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	4	30	theme	argon	568:572	arg1	nitrogen					574:581	argon:nitrogen	568:581	argon:nitrogen	568:581	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	7	31	theme	mechanical	1105:1114	arg1	properties					1116:1125	mechanical properties	1105:1125	mechanical properties	1105:1125	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	4	32	dep	reactive	541:548	arg1	argon					551:555	argon	551:555	argon	551:555	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	3	33	theme	cellulose	412:420	arg1	modification					379:390	the modification	375:390	the modification of microcrystalline cellulose (MCC)	375:426	In this work we demonstrate the modification of microcrystalline cellulose (MCC) by applying the SLP combined with ultrasonication treatments.
30341312	6	34	theme	MCC	899:901	arg1	functionalization					878:894	the surface functionalization	866:894	the surface functionalization of MCC with oxygen or nitrogen moieties	866:934	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	8	35	theme	processing	1175:1184	arg1	method					1186:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	8	36	theme	plasma	1168:1173	arg1	method					1186:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method	1146:1191	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	4	37	used	used	592:595	arg2	plasma					494:499	The plasma	490:499	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas	490:586	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	6	38	theme	surface	870:876	arg1	functionalization					878:894	the surface functionalization	866:894	the surface functionalization of MCC with oxygen or nitrogen moieties	866:934	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	0	39	theme	liquid	60:65	arg1	treatment					67:75	liquid treatment	60:75	liquid treatment	60:75	Cellulose defibrillation and functionalization by plasma in liquid treatment.
30341312	4	40	theme	acetonitrile	628:639	arg1	mixtures					647:654	acetonitrile:water mixtures	628:654	acetonitrile:water mixtures	628:654	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	6	41	dep	transform	801:809	arg1	infrared					811:818	infrared	811:818	transform infrared spectroscopy	801:831	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	6	42	theme	photoelectron	762:774	arg1	spectroscopy					776:787	X-ray photoelectron spectroscopy	756:787	X-ray photoelectron spectroscopy	756:787	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	4	43	dep	gas	584:586	arg1	either					511:516	either	511:516	either	511:516	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	6	44	theme	X-ray	756:760	arg1	spectroscopy					776:787	X-ray photoelectron spectroscopy	756:787	X-ray photoelectron spectroscopy	756:787	Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties.
30341312	7	45	theme	plasma	954:959	arg1	treatment					961:969	the plasma treatment	950:969	the plasma treatment applied	950:977	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	1	46	theme	new	113:115	arg1	method					131:136	a new and promising method	111:136	a new and promising method to modify powder materials	111:163	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	46	theme	new	113:115	arg1	plasma					95:100	Submerged liquid plasma	78:100	Submerged liquid plasma (SLP)	78:106	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	2	47	dep	now	172:174	arg1	Up					166:167	Up	166:167	Up	166:167	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	7	48	theme	poly	980:983	arg1	composites					1005:1014	poly (3-hydroxybutyrate) composites	980:1014	poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers	980:1067	Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB.
30341312	2	49	theme	carbonaceous	219:230	arg1	materials					232:240	carbonaceous materials	219:240	carbonaceous materials	219:240	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	8	50	theme	improved	1310:1317	arg1	ability					1331:1337	an improved reinforcing ability	1307:1337	an improved reinforcing ability of biopolymers	1307:1352	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	2	51	theme	low-cost	283:290	arg1	potential					268:276	great potential	262:276	great potential	262:276	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	2	51	theme	low-cost	283:290	arg1	method					319:324	a low-cost and environmental-friendly method	281:324	a low-cost and environmental-friendly method to modify cellulose	281:344	Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose.
30341312	4	52	dep	argon	551:555	arg1	nitrogen					574:581	argon:nitrogen	568:581	argon:nitrogen	568:581	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	4	52	dep	argon	551:555	arg1	oxygen					558:563	oxygen	558:563	oxygen	558:563	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
30341312	8	53	theme	cellulose	1240:1248	arg1	activation					1226:1235	the activation	1222:1235	the activation of cellulose	1222:1248	This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
30341312	1	54	theme	promising	121:129	arg1	method					131:136	a new and promising method	111:136	a new and promising method to modify powder materials	111:163	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	1	54	theme	promising	121:129	arg1	plasma					95:100	Submerged liquid plasma	78:100	Submerged liquid plasma (SLP)	78:106	Submerged liquid plasma (SLP) is a new and promising method to modify powder materials.
30341312	0	55	from	functionalization	29:45	arg1	treatment					67:75	liquid treatment	60:75	liquid treatment	60:75	Cellulose defibrillation and functionalization by plasma in liquid treatment.
30341312	0	56	dep	Cellulose	0:8	arg1	defibrillation					10:23	defibrillation	10:23	defibrillation	10:23	Cellulose defibrillation and functionalization by plasma in liquid treatment.
30341312	4	57	theme	MCC	600:602	arg1	dispersions					604:614	MCC dispersions	600:614	MCC dispersions	600:614	The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures.
29741364	5	0	theme	order	949:953	arg1	cross-linking					916:928	cross-linking	916:928	cross-linking	916:928	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	5	0	theme	order	949:953	arg1	degree					939:944	high degree	934:944	high degree	934:944	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	6	1	theme	multiscale	1336:1345	arg1	self-organization					1347:1363	multiscale self-organization	1336:1363	multiscale self-organization	1336:1363	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	3	2	theme	cellulose	667:675	arg1	composites					677:686	natural or artificial cellulose composites	645:686	natural or artificial cellulose composites	645:686	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	2	theme	cellulose	667:675	arg1	times					697:701	3-15 times	692:701	3-15 times weaker than the CNFs	692:722	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	1	3	theme	extraordinary	171:183	arg1	properties					196:205	extraordinary mechanical properties	171:205	extraordinary mechanical properties	171:205	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	6	4	with	potential	1267:1275	arg1	self-organization					1347:1363	multiscale self-organization	1336:1363	multiscale self-organization	1336:1363	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	1	5	theme	mechanical	185:194	arg1	properties					196:205	extraordinary mechanical properties	171:205	extraordinary mechanical properties	171:205	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	5	6	theme	synthetic	1099:1107	arg1	materials					1122:1130	known natural or synthetic biopolymeric materials	1082:1130	known natural or synthetic biopolymeric materials	1082:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	6	7	theme	specific	1137:1144	arg1	strength					1146:1153	The specific strength	1133:1153	The specific strength of our CNF fibers engineered at multiscale	1133:1196	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	6	8	theme	materials	1321:1329	arg1	potential					1267:1275	the potential	1263:1275	the potential of sustainable lightweight high-performance materials with multiscale self-organization	1263:1363	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	3	9	from	element	568:574	arg1	systems					586:592	living systems	579:592	living systems	579:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	2	10	theme	difficult	339:347	arg1	challenge					371:379	a difficult materials engineering challenge	337:379	a difficult materials engineering challenge	337:379	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	2	11	theme	materials	349:357	arg1	challenge					371:379	a difficult materials engineering challenge	337:379	a difficult materials engineering challenge	337:379	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	0	12	theme	Macroscale	100:109	arg1	Fibers					111:116	Macroscale Fibers	100:116	Macroscale Fibers	100:116	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	3	13	theme	high	612:615	arg1	strength					617:624	impressively high strength	599:624	impressively high strength	599:624	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	6	14	theme	high-performance	1304:1319	arg1	materials					1321:1329	sustainable lightweight high-performance materials	1280:1329	sustainable lightweight high-performance materials	1280:1329	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	3	15	theme	structural	557:566	arg1	element					568:574	the most abundant structural element	539:574	the most abundant structural element in living systems	539:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	15	theme	structural	557:566	arg1	CNFs					532:535	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	6	16	theme	lightweight	1292:1302	arg1	materials					1321:1329	sustainable lightweight high-performance materials	1280:1329	sustainable lightweight high-performance materials	1280:1329	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	5	17	theme	tensile	1013:1019	arg1	strength					1021:1028	a tensile strength	1011:1028	a tensile strength of 1.57 GPa	1011:1040	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	6	18	theme	fibers	1166:1171	arg1	strength					1146:1153	The specific strength	1133:1153	The specific strength of our CNF fibers engineered at multiscale	1133:1196	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	5	19	theme	Young	969:973	arg1	modulus					977:983	their Young's modulus	963:983	their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa	963:1040	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	6	20	theme	sustainable	1280:1290	arg1	materials					1321:1329	sustainable lightweight high-performance materials	1280:1329	sustainable lightweight high-performance materials	1280:1329	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	5	21	dep	86	1000:1001	arg1	to					997:998	to	997:998	to	997:998	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	4	22	theme	unidirectional	823:836	arg1	alignment					838:846	nearly perfect unidirectional alignment	808:846	nearly perfect unidirectional alignment	808:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	3	23	theme	Cellulose	509:517	arg1	element					568:574	the most abundant structural element	539:574	the most abundant structural element in living systems	539:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	23	theme	Cellulose	509:517	arg1	CNFs					532:535	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	24	theme	living	579:584	arg1	systems					586:592	living systems	579:592	living systems	579:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	5	25	theme	GPa	1038:1040	arg1	GPa					1003:1005	up to 86 GPa	994:1005	up to 86 GPa	994:1005	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	5	25	theme	GPa	1038:1040	arg1	strength					1021:1028	a tensile strength	1011:1028	a tensile strength of 1.57 GPa	1011:1040	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	4	26	theme	macroscale	785:794	arg1	fibers					796:801	macroscale fibers	785:801	macroscale fibers with nearly perfect unidirectional alignment	785:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	2	27	theme	multiscale	441:450	arg1	patterns					452:459	multiscale patterns	441:459	multiscale patterns	441:459	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	0	28	theme	Multiscale	0:9	arg1	Control					11:17	Multiscale Control	0:17	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	0	29	theme	Nanocellulose	22:34	arg1	Assembly					36:43	Nanocellulose Assembly	22:43	Nanocellulose Assembly	22:43	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	0	30	dep	Control	11:17	arg1	Mechanics					87:95	Transferring Remarkable Nanoscale Fibril Mechanics	46:95	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	2	31	theme	engineering	359:369	arg1	challenge					371:379	a difficult materials engineering challenge	337:379	a difficult materials engineering challenge	337:379	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	3	32	theme	natural	645:651	arg1	composites					677:686	natural or artificial cellulose composites	645:686	natural or artificial cellulose composites	645:686	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	32	theme	natural	645:651	arg1	times					697:701	3-15 times	692:701	3-15 times weaker than the CNFs	692:722	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	1	33	theme	defect-free	220:230	arg1	structure					242:250	their defect-free molecular structure	214:250	their defect-free molecular structure	214:250	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	2	34	theme	larger	494:499	arg1	scales					501:506	larger scales	494:506	larger scales	494:506	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	3	35	theme	weaker	703:708	arg1	composites					677:686	natural or artificial cellulose composites	645:686	natural or artificial cellulose composites	645:686	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	35	theme	weaker	703:708	arg1	times					697:701	3-15 times	692:701	3-15 times weaker than the CNFs	692:722	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	1	36	theme	Nanoscale	119:127	arg1	blocks					138:143	Nanoscale building blocks	119:143	Nanoscale building blocks of many materials	119:161	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	1	37	theme	molecular	232:240	arg1	structure					242:250	their defect-free molecular structure	214:250	their defect-free molecular structure	214:250	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	4	38	theme	flow-assisted	745:757	arg1	organization					759:770	the flow-assisted organization	741:770	the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment	741:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	5	39	theme	biopolymeric	1109:1120	arg1	materials					1122:1130	known natural or synthetic biopolymeric materials	1082:1130	known natural or synthetic biopolymeric materials	1082:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	2	40	theme	high	274:277	arg1	properties					290:299	these high mechanical properties	268:299	these high mechanical properties	268:299	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	3	41	theme	nanofibrils	519:529	arg1	element					568:574	the most abundant structural element	539:574	the most abundant structural element in living systems	539:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	41	theme	nanofibrils	519:529	arg1	CNFs					532:535	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	0	42	theme	Assembly	36:43	arg1	Control					11:17	Multiscale Control	0:17	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	5	43	theme	Efficient	849:857	arg1	transfer					866:873	Efficient stress transfer	849:873	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order	849:953	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	5	44	theme	materials	1122:1130	arg1	properties					1068:1077	the mechanical properties	1053:1077	the mechanical properties of known natural or synthetic biopolymeric materials	1053:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	0	45	theme	Remarkable	59:68	arg1	Mechanics					87:95	Transferring Remarkable Nanoscale Fibril Mechanics	46:95	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	4	46	with	fibers	796:801	arg1	alignment					838:846	nearly perfect unidirectional alignment	808:846	nearly perfect unidirectional alignment	808:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	5	47	theme	individual	894:903	arg1	CNF					905:907	individual CNF	894:907	individual CNF	894:907	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	5	48	theme	mechanical	1057:1066	arg1	properties					1068:1077	the mechanical properties	1053:1077	the mechanical properties of known natural or synthetic biopolymeric materials	1053:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	6	49	theme	glass	1239:1243	arg1	fibers					1245:1250	glass fibers	1239:1250	glass fibers	1239:1250	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	1	50	theme	building	129:136	arg1	blocks					138:143	Nanoscale building blocks	119:143	Nanoscale building blocks of many materials	119:161	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	0	51	theme	Transferring	46:57	arg1	Mechanics					87:95	Transferring Remarkable Nanoscale Fibril Mechanics	46:95	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	5	52	theme	known	1082:1086	arg1	materials					1122:1130	known natural or synthetic biopolymeric materials	1082:1130	known natural or synthetic biopolymeric materials	1082:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	5	53	from	macroscale	880:889	arg1	transfer					866:873	Efficient stress transfer	849:873	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order	849:953	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	3	54	theme	abundant	548:555	arg1	element					568:574	the most abundant structural element	539:574	the most abundant structural element in living systems	539:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	54	theme	abundant	548:555	arg1	CNFs					532:535	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	55	contain	has	595:597	arg2	stiffness					630:638	stiffness	630:638	stiffness	630:638	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	55	contain	has	595:597	arg1	element					568:574	the most abundant structural element	539:574	the most abundant structural element in living systems	539:592	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	55	contain	has	595:597	arg2	strength					617:624	impressively high strength	599:624	impressively high strength	599:624	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	55	contain	has	595:597	arg1	CNFs					532:535	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs)	509:536	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	5	56	theme	stress	859:864	arg1	transfer					866:873	Efficient stress transfer	849:873	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order	849:953	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	2	57	theme	macroscopic	304:314	arg1	materials					316:324	macroscopic materials	304:324	macroscopic materials	304:324	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	4	58	theme	CNFs	775:778	arg1	organization					759:770	the flow-assisted organization	741:770	the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment	741:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	3	59	theme	artificial	656:665	arg1	composites					677:686	natural or artificial cellulose composites	645:686	natural or artificial cellulose composites	645:686	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	3	59	theme	artificial	656:665	arg1	times					697:701	3-15 times	692:701	3-15 times weaker than the CNFs	692:722	Cellulose nanofibrils (CNFs), the most abundant structural element in living systems, has impressively high strength and stiffness, but natural or artificial cellulose composites are 3-15 times weaker than the CNFs.
29741364	5	60	theme	natural	1088:1094	arg1	materials					1122:1130	known natural or synthetic biopolymeric materials	1082:1130	known natural or synthetic biopolymeric materials	1082:1130	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	4	61	theme	perfect	815:821	arg1	alignment					838:846	nearly perfect unidirectional alignment	808:846	nearly perfect unidirectional alignment	808:846	Here, we report the flow-assisted organization of CNFs into macroscale fibers with nearly perfect unidirectional alignment.
29741364	1	62	theme	many	148:151	arg1	materials					153:161	many materials	148:161	many materials	148:161	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	6	63	theme	CNF	1162:1164	arg1	fibers					1166:1171	our CNF fibers	1158:1171	our CNF fibers engineered at multiscale	1158:1196	The specific strength of our CNF fibers engineered at multiscale also exceeds that of metals, alloys, and glass fibers, enhancing the potential of sustainable lightweight high-performance materials with multiscale self-organization.
29741364	0	64	theme	Fibril	80:85	arg1	Mechanics					87:95	Transferring Remarkable Nanoscale Fibril Mechanics	46:95	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	2	65	theme	properties	290:299	arg1	Translation					253:263	Translation	253:263	Translation of these high mechanical properties to macroscopic materials	253:324	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	1	66	theme	materials	153:161	arg1	blocks					138:143	Nanoscale building blocks	119:143	Nanoscale building blocks of many materials	119:161	Nanoscale building blocks of many materials exhibit extraordinary mechanical properties due to their defect-free molecular structure.
29741364	5	67	theme	high	934:937	arg1	degree					939:944	high degree	934:944	high degree	934:944	Efficient stress transfer from macroscale to individual CNF due to cross-linking and high degree of order enables their Young's modulus to reach up to 86 GPa and a tensile strength of 1.57 GPa, exceeding the mechanical properties of known natural or synthetic biopolymeric materials.
29741364	0	68	theme	Nanoscale	70:78	arg1	Mechanics					87:95	Transferring Remarkable Nanoscale Fibril Mechanics	46:95	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.	0:117	Multiscale Control of Nanocellulose Assembly: Transferring Remarkable Nanoscale Fibril Mechanics to Macroscale Fibers.
29741364	2	69	theme	mechanical	279:288	arg1	properties					290:299	these high mechanical properties	268:299	these high mechanical properties	268:299	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
29741364	2	70	theme	building	420:427	arg1	blocks					429:434	these building blocks	414:434	these building blocks	414:434	Translation of these high mechanical properties to macroscopic materials represents a difficult materials engineering challenge due to the necessity to organize these building blocks into multiscale patterns and mitigate defects emerging at larger scales.
30925991	6	0	theme	good	1152:1155	arg1	behaviors					1177:1185	good resistive switching behaviors	1152:1185	good resistive switching behaviors	1152:1185	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	7	1	theme	artificial	1582:1591	arg1	intelligence					1593:1604	artificial intelligence	1582:1604	artificial intelligence	1582:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	2	theme	electronic	1544:1553	arg1	skins					1555:1559	electronic skins	1544:1559	electronic skins in the coming age of artificial intelligence	1544:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	3	3	theme	oxide	627:631	arg1	nanocomposite					642:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	1	4	theme	great	315:319	arg1	interest					321:328	great interest	315:328	great interest	315:328	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	3	5	theme	carboxymethyl	594:606	arg1	nanocomposite					642:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	5	6	theme	write-once-read-many-times	929:954	arg1	characteristics					973:987	brilliant write-once-read-many-times (WORM) switching characteristics	919:987	brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability	919:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	6	theme	write-once-read-many-times	929:954	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	6	7	theme	Al/CMC-GO/Al/PET	1217:1232	arg1	substrate					1206:1214	soft PET substrate	1197:1214	soft PET substrate (Al/CMC-GO/Al/PET structure)	1197:1243	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	6	7	theme	Al/CMC-GO/Al/PET	1217:1232	arg1	structure					1234:1242	Al/CMC-GO/Al/PET structure	1217:1242	Al/CMC-GO/Al/PET structure	1217:1242	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	4	8	theme	CMC	743:745	arg1	gelation					731:738	the gelation	727:738	the gelation of CMC and GO molecules	727:762	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	2	9	theme	natural	350:356	arg1	potential					407:415	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	2	9	theme	natural	350:356	arg1	polymers					358:365	the most common natural polymers	334:365	the most common natural polymers	334:365	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	1	10	theme	flexible	222:229	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	3	11	theme	CMC-GO	634:639	arg1	nanocomposite					642:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	5	12	theme	Al/CMC-GO/Al/SiO2	882:898	arg1	structure					900:908	the Al/CMC-GO/Al/SiO2 structure	878:908	the Al/CMC-GO/Al/SiO2 structure	878:908	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	1	13	theme	biocompatible	247:259	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	5	14	from	base	837:840	arg1	CMC					857:859	CMC	857:859	CMC	857:859	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	14	from	base	837:840	arg1	GO					850:851	GO	850:851	GO	850:851	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	15	theme	high	997:1000	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	15	theme	high	997:1000	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	16	from	stretchable	1497:1507	arg1	age					1575:1577	the coming age	1564:1577	the coming age of artificial intelligence	1564:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	17	theme	data	1454:1457	arg1	stretchable					1497:1507	portable stretchable displays	1488:1516	portable stretchable displays	1488:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	17	theme	data	1454:1457	arg1	electronics					1528:1538	wearable electronics	1519:1538	wearable electronics	1519:1538	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	17	theme	data	1454:1457	arg1	applications					1467:1478	future biodegradable data storage applications	1433:1478	future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence	1433:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	17	theme	data	1454:1457	arg1	skins					1555:1559	electronic skins	1544:1559	electronic skins in the coming age of artificial intelligence	1544:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	4	18	theme	memory	792:797	arg1	behaviors					799:807	the excellent memory behaviors	778:807	the excellent memory behaviors	778:807	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	5	19	theme	current	1009:1015	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	19	theme	current	1009:1015	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	20	theme	future	1433:1438	arg1	stretchable					1497:1507	portable stretchable displays	1488:1516	portable stretchable displays	1488:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	20	theme	future	1433:1438	arg1	electronics					1528:1538	wearable electronics	1519:1538	wearable electronics	1519:1538	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	20	theme	future	1433:1438	arg1	applications					1467:1478	future biodegradable data storage applications	1433:1478	future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence	1433:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	20	theme	future	1433:1438	arg1	skins					1555:1559	electronic skins	1544:1559	electronic skins in the coming age of artificial intelligence	1544:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	4	21	theme	GO	751:752	arg1	gelation					731:738	the gelation	727:738	the gelation of CMC and GO molecules	727:762	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	5	22	theme	devices	829:835	arg1	base					837:840	devices base	829:840	devices base on pure GO and CMC	829:859	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	23	theme	switching	963:971	arg1	characteristics					973:987	brilliant write-once-read-many-times (WORM) switching characteristics	919:987	brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability	919:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	23	theme	switching	963:971	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	24	from	electronics	1528:1538	arg1	age					1575:1577	the coming age	1564:1577	the coming age of artificial intelligence	1564:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	0	25	theme	nonvolatile	90:100	arg1	devices					129:135	flexible nonvolatile resistive switching memory devices	81:135	flexible nonvolatile resistive switching memory devices	81:135	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	1	26	theme	promising	190:198	arg1	polymers					209:216	promising building polymers	190:216	promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices	190:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	7	27	theme	oxide-based	1285:1295	arg1	nanocomposites					1305:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	5	28	theme	switching	1036:1044	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	28	theme	switching	1036:1044	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	1	29	dep	Nowadays	138:145	arg1	interest					321:328	great interest	315:328	great interest	315:328	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	7	30	theme	designed	1257:1264	arg1	nanocomposites					1305:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	6	31	theme	high	1131:1134	arg1	flexibility					1136:1146	high flexibility	1131:1146	high flexibility	1131:1146	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	2	32	theme	easy	459:462	arg1	processing					464:473	the easy processing	455:473	the easy processing	455:473	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	0	33	theme	carboxymethyl	15:27	arg1	cellulose					29:37	carboxymethyl cellulose	15:37	carboxymethyl cellulose	15:37	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	7	34	dep	stretchable	1497:1507	arg1	displays					1509:1516	displays	1509:1516	displays	1509:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	5	35	theme	brilliant	919:927	arg1	characteristics					973:987	brilliant write-once-read-many-times (WORM) switching characteristics	919:987	brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability	919:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	35	theme	brilliant	919:927	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	36	theme	durability	1089:1098	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	36	theme	durability	1089:1098	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	37	theme	memory	1388:1393	arg1	devices					1395:1401	memory devices	1388:1401	memory devices	1388:1401	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	3	38	theme	write-once-read-many-times	516:541	arg1	devices					563:569	write-once-read-many-times resistive switching devices	516:569	write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	516:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	0	39	theme	oxide	52:56	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of carboxymethyl cellulose and graphene oxide	0:56	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	7	40	theme	large	1359:1363	arg1	manufacturing					1371:1383	large scale manufacturing	1359:1383	large scale manufacturing of memory devices	1359:1401	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	1	41	theme	friendly	281:288	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	7	42	theme	intelligence	1593:1604	arg1	age					1575:1577	the coming age	1564:1577	the coming age of artificial intelligence	1564:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	5	43	theme	stability	1075:1083	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	43	theme	stability	1075:1083	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	6	44	theme	resistive	1157:1165	arg1	behaviors					1177:1185	good resistive switching behaviors	1152:1185	good resistive switching behaviors	1152:1185	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	7	45	theme	polymer	1297:1303	arg1	nanocomposites					1305:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	3	46	theme	switching	553:561	arg1	devices					563:569	write-once-read-many-times resistive switching devices	516:569	write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	516:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	3	47	theme	cellulose-graphene	608:625	arg1	nanocomposite					642:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	5	48	with	device	866:871	arg1	structure					900:908	the Al/CMC-GO/Al/SiO2 structure	878:908	the Al/CMC-GO/Al/SiO2 structure	878:908	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	49	theme	coming	1568:1573	arg1	age					1575:1577	the coming age	1564:1577	the coming age of artificial intelligence	1564:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	6	50	theme	soft	1197:1200	arg1	substrate					1206:1214	soft PET substrate	1197:1214	soft PET substrate (Al/CMC-GO/Al/PET structure)	1197:1243	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	6	50	theme	soft	1197:1200	arg1	structure					1234:1242	Al/CMC-GO/Al/PET structure	1217:1242	Al/CMC-GO/Al/PET structure	1217:1242	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	3	51	theme	biodegradable	580:592	arg1	nanocomposite					642:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	580:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	1	52	theme	building	200:207	arg1	polymers					209:216	promising building polymers	190:216	promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices	190:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	7	53	theme	wearable	1519:1526	arg1	electronics					1528:1538	wearable electronics	1519:1538	wearable electronics	1519:1538	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	2	54	theme	common	343:348	arg1	potential					407:415	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	2	54	theme	common	343:348	arg1	polymers					358:365	the most common natural polymers	334:365	the most common natural polymers	334:365	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	6	55	theme	PET	1202:1204	arg1	substrate					1206:1214	soft PET substrate	1197:1214	soft PET substrate (Al/CMC-GO/Al/PET structure)	1197:1243	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	6	55	theme	PET	1202:1204	arg1	structure					1234:1242	Al/CMC-GO/Al/PET structure	1217:1242	Al/CMC-GO/Al/PET structure	1217:1242	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	4	56	theme	hybridization	697:709	arg1	sites					711:715	The hybridization sites	693:715	The hybridization sites formed by the gelation of CMC and GO molecules	693:762	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	5	57	theme	WORM	957:960	arg1	characteristics					973:987	brilliant write-once-read-many-times (WORM) switching characteristics	919:987	brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability	919:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	57	theme	WORM	957:960	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	58	theme	portable	1488:1495	arg1	stretchable					1497:1507	portable stretchable displays	1488:1516	portable stretchable displays	1488:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	59	theme	cellulose-graphene	1266:1283	arg1	nanocomposites					1305:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites	1246:1318	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	4	60	dep	CMC	743:745	arg1	molecules					754:762	molecules	754:762	molecules	754:762	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	5	61	theme	ON/OFF	1002:1007	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	61	theme	ON/OFF	1002:1007	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	62	theme	storage	1459:1465	arg1	stretchable					1497:1507	portable stretchable displays	1488:1516	portable stretchable displays	1488:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	62	theme	storage	1459:1465	arg1	electronics					1528:1538	wearable electronics	1519:1538	wearable electronics	1519:1538	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	62	theme	storage	1459:1465	arg1	applications					1467:1478	future biodegradable data storage applications	1433:1478	future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence	1433:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	62	theme	storage	1459:1465	arg1	skins					1555:1559	electronic skins	1544:1559	electronic skins in the coming age of artificial intelligence	1544:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	4	63	theme	excellent	782:790	arg1	behaviors					799:807	the excellent memory behaviors	778:807	the excellent memory behaviors	778:807	The hybridization sites formed by the gelation of CMC and GO molecules contribute to the excellent memory behaviors.
30925991	0	64	theme	flexible	81:88	arg1	devices					129:135	flexible nonvolatile resistive switching memory devices	81:135	flexible nonvolatile resistive switching memory devices	81:135	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	3	65	theme	first	681:685	arg1	time					687:690	the first time	677:690	the first time	677:690	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	1	66	theme	natural	166:172	arg1	biomaterials					174:185	natural biomaterials	166:185	natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices	166:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	7	67	theme	biodegradable	1440:1452	arg1	stretchable					1497:1507	portable stretchable displays	1488:1516	portable stretchable displays	1488:1516	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	67	theme	biodegradable	1440:1452	arg1	electronics					1528:1538	wearable electronics	1519:1538	wearable electronics	1519:1538	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	67	theme	biodegradable	1440:1452	arg1	applications					1467:1478	future biodegradable data storage applications	1433:1478	future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence	1433:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	7	67	theme	biodegradable	1440:1452	arg1	skins					1555:1559	electronic skins	1544:1559	electronic skins in the coming age of artificial intelligence	1544:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	0	68	theme	switching	112:120	arg1	devices					129:135	flexible nonvolatile resistive switching memory devices	81:135	flexible nonvolatile resistive switching memory devices	81:135	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	0	69	theme	resistive	102:110	arg1	devices					129:135	flexible nonvolatile resistive switching memory devices	81:135	flexible nonvolatile resistive switching memory devices	81:135	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	0	70	theme	memory	122:127	arg1	devices					129:135	flexible nonvolatile resistive switching memory devices	81:135	flexible nonvolatile resistive switching memory devices	81:135	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	5	71	theme	low	1032:1034	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	71	theme	low	1032:1034	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	72	from	skins	1555:1559	arg1	age					1575:1577	the coming age	1564:1577	the coming age of artificial intelligence	1564:1604	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	2	73	contain	have	398:401	arg2	potential					407:415	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability	403:507	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	2	73	contain	have	398:401	arg2	polymers					358:365	the most common natural polymers	334:365	the most common natural polymers	334:365	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	2	73	contain	have	398:401	arg1	cellulose					368:376	cellulose	368:376	cellulose	368:376	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	2	73	contain	have	398:401	arg1	derivatives					386:396	its derivatives	382:396	its derivatives	382:396	As the most common natural polymers, cellulose and its derivatives have the potential to be applied in the devices owing to the easy processing, nontoxicity and biodegradability.
30925991	1	74	theme	biodegradable	232:244	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	0	75	theme	cellulose	29:37	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of carboxymethyl cellulose and graphene oxide	0:56	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	5	76	theme	˜105	1026:1029	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	76	theme	˜105	1026:1029	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	7	77	theme	devices	1395:1401	arg1	manufacturing					1371:1383	large scale manufacturing	1359:1383	large scale manufacturing of memory devices	1359:1401	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	0	78	theme	graphene	43:50	arg1	oxide					52:56	graphene oxide	43:56	graphene oxide	43:56	Fabrication of carboxymethyl cellulose and graphene oxide bio-nanocomposites for flexible nonvolatile resistive switching memory devices.
30925991	7	79	theme	scale	1365:1369	arg1	manufacturing					1371:1383	large scale manufacturing	1359:1383	large scale manufacturing of memory devices	1359:1401	This newly designed cellulose-graphene oxide-based polymer nanocomposites are quite cheap and easy processed for large scale manufacturing of memory devices and can further contribute to future biodegradable data storage applications such as portable stretchable displays, wearable electronics and electronic skins in the coming age of artificial intelligence.
30925991	1	80	theme	biomaterials	174:185	arg1	development					151:161	the development	147:161	the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices	147:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	3	81	theme	resistive	543:551	arg1	devices					563:569	write-once-read-many-times resistive switching devices	516:569	write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite	516:654	Here, write-once-read-many-times resistive switching devices based on biodegradable carboxymethyl cellulose-graphene oxide (CMC-GO) nanocomposite are demonstrated for the first time.
30925991	5	82	theme	2.22 V	1057:1062	arg1	ratio					1017:1021	high ON/OFF current ratio	997:1021	high ON/OFF current ratio of ˜105	997:1029	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	5	82	theme	2.22 V	1057:1062	arg1	voltage					1046:1052	low switching voltage	1032:1052	low switching voltage of 2.22 V, excellent stability and durability	1032:1098	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	1	83	theme	environmentally	265:279	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
30925991	5	84	theme	excellent	1065:1073	arg1	stability					1075:1083	excellent stability	1065:1083	excellent stability	1065:1083	When compared with devices base on pure GO and CMC, the device with the Al/CMC-GO/Al/SiO2 structure exhibits brilliant write-once-read-many-times (WORM) switching characteristics such as high ON/OFF current ratio of ˜105, low switching voltage of 2.22 V, excellent stability and durability.
30925991	6	85	theme	switching	1167:1175	arg1	behaviors					1177:1185	good resistive switching behaviors	1152:1185	good resistive switching behaviors	1152:1185	What's more, the device shows high flexibility and good resistive switching behaviors even with soft PET substrate (Al/CMC-GO/Al/PET structure).
30925991	1	86	theme	electronic	290:299	arg1	devices					301:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	flexible, biodegradable, biocompatible and environmentally friendly electronic devices	222:307	Nowadays the development of natural biomaterials as promising building polymers for flexible, biodegradable, biocompatible and environmentally friendly electronic devices is of great interest.
29801844	2	0	theme	liquid-polymer	337:350	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	0	theme	liquid-polymer	337:350	arg1	ratio					352:356	liquid-polymer ratio	337:356	liquid-polymer ratio	337:356	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	6	1	theme	FTIR	859:862	arg1	Results					848:854	Results	848:854	Results of FTIR, XRD and TGA	848:875	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	0	2	theme	release	79:85	arg1	studies					87:93	in-vitro drug release studies	65:93	in-vitro drug release studies	65:93	Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.
29801844	7	3	theme	5-FU	972:975	arg1	drug					977:980	5-FU drug	972:980	5-FU drug	972:980	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	1	4	theme	PB	176:177	arg1	design					180:185	Plackett-Burman (PB) design	159:185	Plackett-Burman (PB) design	159:185	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	7	5	theme	Faster	954:959	arg1	release					961:967	Faster release	954:967	Faster release of 5-FU drug	954:980	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	4	6	theme	CAA	731:733	arg1	concentration					735:747	CAA concentration	731:747	CAA concentration (8.5%)	731:754	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	4	6	theme	CAA	731:733	arg1	%					753:753	8.5%	750:753	8.5%	750:753	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	3	7	theme	concentration	505:517	arg1	effects					486:492	the interaction effects	470:492	the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP)	470:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	4	8	theme	ethanol	676:682	arg1	concentration					684:696	ethanol concentration	676:696	ethanol concentration (80%)	676:702	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	4	8	theme	ethanol	676:682	arg1	%					701:701	80%	699:701	80%	699:701	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	7	9	theme	order	1107:1111	arg1	kinetics					1113:1120	zero order kinetics	1102:1120	zero order kinetics model	1102:1126	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	3	10	from	degree	570:575	arg1	CMP					627:629	CMP	627:629	CMP	627:629	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	10	from	degree	570:575	arg1	pectin					619:624	carboxymethylated pectin	601:624	carboxymethylated pectin (CMP)	601:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	4	11	theme	DS	641:642	arg1	value					644:648	Maximum DS value	633:648	Maximum DS value of 0.496	633:657	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	5	12	theme	synthesized	779:789	arg1	CMP					791:793	The synthesized CMP	775:793	The synthesized CMP	775:793	The synthesized CMP was characterized by FT-IR, XRD, TGA and viscometer.
29801844	3	13	from	effects	486:492	arg1	degree					570:575	degree	570:575	degree of substitution (DS) in carboxymethylated pectin (CMP)	570:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	2	14	theme	CAA	379:381	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	14	theme	CAA	379:381	arg1	concentration					383:395	CAA concentration	379:395	CAA concentration	379:395	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	7	15	theme	pectin-chitosan	1042:1056	arg1	nanoparticles					1058:1070	pectin-chitosan nanoparticles	1042:1070	pectin-chitosan nanoparticles	1042:1070	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	3	16	theme	ethanol	497:503	arg1	concentration					505:517	ethanol concentration	497:517	ethanol concentration	497:517	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	17	theme	Central	421:427	arg1	design					439:444	Central composite design	421:444	Central composite design (CCD)	421:450	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	17	theme	Central	421:427	arg1	CCD					447:449	CCD	447:449	CCD	447:449	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	7	18	theme	drug	1080:1083	arg1	release					1085:1091	the drug release	1076:1091	the drug release	1076:1091	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	7	19	theme	kinetics	1113:1120	arg1	model					1122:1126	zero order kinetics model	1102:1126	zero order kinetics model	1102:1126	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	2	20	theme	process	295:301	arg1	time					414:417	time	414:417	time	414:417	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	ratio					352:356	liquid-polymer ratio	337:356	liquid-polymer ratio	337:356	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	concentration					383:395	CAA concentration	379:395	CAA concentration	379:395	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	temperature					398:408	temperature	398:408	temperature	398:408	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	concentration					322:334	ethanol concentration	314:334	ethanol concentration	314:334	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	20	theme	process	295:301	arg1	concentration					364:376	NaOH concentration	359:376	NaOH concentration	359:376	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	time					414:417	time	414:417	time	414:417	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	ratio					352:356	liquid-polymer ratio	337:356	liquid-polymer ratio	337:356	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	concentration					383:395	CAA concentration	379:395	CAA concentration	379:395	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	temperature					398:408	temperature	398:408	temperature	398:408	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	concentration					322:334	ethanol concentration	314:334	ethanol concentration	314:334	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	21	dep	variables	303:311	arg1	concentration					364:376	NaOH concentration	359:376	NaOH concentration	359:376	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	22	theme	NaOH	359:362	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	22	theme	NaOH	359:362	arg1	concentration					364:376	NaOH concentration	359:376	NaOH concentration	359:376	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	7	23	theme	CMP-chitosan	1000:1011	arg1	nanoparticles					1013:1025	CMP-chitosan nanoparticles	1000:1025	CMP-chitosan nanoparticles	1000:1025	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	0	24	dep	Optimization	30:41	arg1	Carboxymethylation					0:17	Carboxymethylation	0:17	Carboxymethylation of pectin	0:27	Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.
29801844	1	25	theme	sequential	100:109	arg1	optimization					111:122	The sequential optimization	96:122	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM)	96:224	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	1	26	theme	surface	200:206	arg1	methodology					208:218	response surface methodology	191:218	response surface methodology (RSM)	191:224	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	1	26	theme	surface	200:206	arg1	RSM					221:223	RSM	221:223	RSM	221:223	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	0	27	theme	pectin	22:27	arg1	Carboxymethylation					0:17	Carboxymethylation	0:17	Carboxymethylation of pectin	0:27	Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.
29801844	3	28	theme	concentration	525:537	arg1	effects					486:492	the interaction effects	470:492	the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP)	470:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	4	29	theme	Maximum	633:639	arg1	value					644:648	Maximum DS value	633:648	Maximum DS value of 0.496	633:657	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	2	30	theme	ethanol	314:320	arg1	variables					303:311	the six process variables	287:311	the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time)	287:418	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	2	30	theme	ethanol	314:320	arg1	concentration					322:334	ethanol concentration	314:334	ethanol concentration	314:334	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	3	31	theme	carboxymethylated	601:617	arg1	CMP					627:629	CMP	627:629	CMP	627:629	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	31	theme	carboxymethylated	601:617	arg1	pectin					619:624	carboxymethylated pectin	601:624	carboxymethylated pectin (CMP)	601:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	32	theme	time	562:565	arg1	effects					486:492	the interaction effects	470:492	the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP)	470:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	1	33	theme	response	191:198	arg1	methodology					208:218	response surface methodology	191:218	response surface methodology (RSM)	191:224	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	1	33	theme	response	191:198	arg1	RSM					221:223	RSM	221:223	RSM	221:223	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	3	34	theme	CAA	540:542	arg1	concentration					544:556	CAA concentration	540:556	CAA concentration	540:556	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	7	35	theme	drug	977:980	arg1	release					961:967	Faster release	954:967	Faster release of 5-FU drug	954:980	Faster release of 5-FU drug was observed with CMP-chitosan nanoparticles as compared to pectin-chitosan nanoparticles and the drug release followed zero order kinetics model.
29801844	3	36	theme	concentration	544:556	arg1	effects					486:492	the interaction effects	470:492	the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP)	470:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	4	37	theme	0.496	653:657	arg1	value					644:648	Maximum DS value	633:648	Maximum DS value of 0.496	633:657	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	3	38	used	used	456:459	arg2	CCD					447:449	CCD	447:449	CCD	447:449	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	38	used	used	456:459	arg2	design					439:444	Central composite design	421:444	Central composite design (CCD)	421:450	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	1	39	theme	carboxymethylation	127:144	arg1	optimization					111:122	The sequential optimization	96:122	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM)	96:224	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	3	40	theme	substitution	580:591	arg1	degree					570:575	degree	570:575	degree of substitution (DS) in carboxymethylated pectin (CMP)	570:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	2	41	theme	PB	254:255	arg1	design					257:262	PB design	254:262	PB design	254:262	PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time).
29801844	3	42	theme	NaOH	520:523	arg1	concentration					525:537	NaOH concentration	520:537	NaOH concentration	520:537	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	6	43	theme	TGA	873:875	arg1	Results					848:854	Results	848:854	Results of FTIR, XRD and TGA	848:875	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	6	44	dep	modification	891:902	arg1	methylated					942:951	methylated	942:951	highly methylated	935:951	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	6	44	dep	modification	891:902	arg1	made					904:907	made	904:907	made in the pectin polymer	904:929	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	4	45	theme	NaOH	705:708	arg1	concentration					710:722	NaOH concentration	705:722	NaOH concentration (38%)	705:728	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	4	45	theme	NaOH	705:708	arg1	%					727:727	38%	725:727	38%	725:727	Maximum DS value of 0.496 was predicted at ethanol concentration (80%), NaOH concentration (38%), CAA concentration (8.5%) and time (60 min).
29801844	1	46	theme	pectin	149:154	arg1	carboxymethylation					127:144	carboxymethylation	127:144	carboxymethylation of pectin	127:154	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
29801844	0	47	theme	drug	74:77	arg1	studies					87:93	in-vitro drug release studies	65:93	in-vitro drug release studies	65:93	Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.
29801844	3	48	theme	composite	429:437	arg1	design					439:444	Central composite design	421:444	Central composite design (CCD)	421:450	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	48	theme	composite	429:437	arg1	CCD					447:449	CCD	447:449	CCD	447:449	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	3	49	theme	interaction	474:484	arg1	effects					486:492	the interaction effects	470:492	the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP)	470:630	Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).
29801844	6	50	theme	pectin	916:921	arg1	polymer					923:929	the pectin polymer	912:929	the pectin polymer	912:929	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	6	51	theme	XRD	865:867	arg1	Results					848:854	Results	848:854	Results of FTIR, XRD and TGA	848:875	Results of FTIR, XRD and TGA confirmed the modification made in the pectin polymer and highly methylated.
29801844	0	52	theme	in-vitro	65:72	arg1	studies					87:93	in-vitro drug release studies	65:93	in-vitro drug release studies	65:93	Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.
29801844	1	53	theme	Plackett-Burman	159:173	arg1	design					180:185	Plackett-Burman (PB) design	159:185	Plackett-Burman (PB) design	159:185	The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study.
31362023	7	0	theme	soil	1053:1056	arg1	test					1065:1068	indoor soil burial test	1046:1068	indoor soil burial test	1046:1068	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	4	1	theme	water	748:752	arg1	sensitivity					754:764	decreased water sensitivity	738:764	decreased water sensitivity	738:764	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	8	2	theme	PVA/BRS	1164:1170	arg1	films					1191:1195	both the PVA/BRS and PVA/BRS/sAgNPs films	1155:1195	films	1191:1195	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	8	2	theme	PVA/BRS	1164:1170	arg1	biodegradable					1210:1222	biodegradable	1210:1222	biodegradable	1210:1222	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	1	3	theme	film	272:275	arg1	preparation					214:224	a one-step preparation	203:224	a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	203:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	6	4	theme	environmental	963:975	arg1	microorganisms					977:990	environmental microorganisms	963:990	environmental microorganisms	963:990	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	8	5	theme	packaging	1284:1292	arg1	material					1294:1301	cost effective food packaging material	1264:1301	cost effective food packaging material	1264:1301	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	4	6	theme	decreased	738:746	arg1	sensitivity					754:764	decreased water sensitivity	738:764	decreased water sensitivity	738:764	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	5	7	theme	boiled	779:784	arg1	starch					791:796	boiled rice starch	779:796	boiled rice starch	779:796	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	5	8	theme	rice	786:789	arg1	starch					791:796	boiled rice starch	779:796	boiled rice starch	779:796	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	1	9	theme	sunlight	375:382	arg1	irradiation					384:394	sunlight irradiation	375:394	sunlight irradiation	375:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	7	10	theme	films	1025:1029	arg1	Biodegradation					993:1006	Biodegradation	993:1006	Biodegradation of the composite films	993:1029	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	8	11	contain	have	1234:1237	arg1	films					1191:1195	both the PVA/BRS and PVA/BRS/sAgNPs films	1155:1195	films	1191:1195	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	8	11	contain	have	1234:1237	arg1	biodegradable					1210:1222	biodegradable	1210:1222	biodegradable	1210:1222	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	8	11	contain	have	1234:1237	arg2	application					1249:1259	promising application	1239:1259	promising application	1239:1259	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	8	12	theme	antimicrobial	1333:1345	arg1	property					1347:1354	marked antimicrobial property	1326:1354	marked antimicrobial property	1326:1354	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	5	13	theme	starch	791:796	arg1	Presence					767:774	Presence	767:774	Presence of boiled rice starch and sAgNPs	767:807	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	1	14	theme	irradiation	384:394	arg1	presence					363:370	the presence	359:370	the presence of sunlight irradiation	359:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	8	15	theme	marked	1326:1331	arg1	property					1347:1354	marked antimicrobial property	1326:1354	marked antimicrobial property	1326:1354	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	3	16	theme	physical	571:578	arg1	interactions					580:591	physical interactions	571:591	physical interactions among the components PVA, BRS and sAgNPs	571:632	Further characterization has confirmed the presence of physical interactions among the components PVA, BRS and sAgNPs.
31362023	4	17	theme	mechanical	699:708	arg1	properties					722:731	improved mechanical and optical properties	690:731	improved mechanical and optical properties	690:731	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	2	18	theme	resonance	490:498	arg1	peak					500:503	a characteristic surface plasmon resonance peak	457:503	a characteristic surface plasmon resonance peak at 439 nm	457:513	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	2	19	from	439 nm	508:513	arg1	peak					500:503	a characteristic surface plasmon resonance peak	457:503	a characteristic surface plasmon resonance peak at 439 nm	457:513	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	8	20	theme	food	1279:1282	arg1	material					1294:1301	cost effective food packaging material	1264:1301	cost effective food packaging material	1264:1301	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	2	21	theme	surface	474:480	arg1	resonance					490:498	surface plasmon resonance	474:498	a characteristic surface plasmon resonance peak at 439 nm	457:513	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	4	22	theme	improved	690:697	arg1	properties					722:731	improved mechanical and optical properties	690:731	improved mechanical and optical properties	690:731	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	2	23	theme	plasmon	482:488	arg1	resonance					490:498	surface plasmon resonance	474:498	a characteristic surface plasmon resonance peak at 439 nm	457:513	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	7	24	theme	weight	1109:1114	arg1	loss					1116:1119	weight loss	1109:1119	weight loss	1109:1119	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	7	25	theme	composite	1015:1023	arg1	films					1025:1029	the composite films	1011:1029	the composite films	1011:1029	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	6	26	theme	barrier	938:944	arg1	property					946:953	superior barrier property	929:953	superior barrier property	929:953	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	4	27	theme	optical	714:720	arg1	properties					722:731	improved mechanical and optical properties	690:731	improved mechanical and optical properties	690:731	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	0	28	theme	One-step	0:7	arg1	synthesis					9:17	One-step synthesis	0:17	One-step synthesis of eco-friendly boiled rice starch	0:52	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	0	29	theme	eco-friendly	22:33	arg1	starch					47:52	eco-friendly boiled rice starch	22:52	eco-friendly boiled rice starch	22:52	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	1	30	theme	generated	301:309	arg1	PVA/BRS/sAgNPs					333:346	PVA/BRS/sAgNPs	333:346	PVA/BRS/sAgNPs	333:346	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	1	30	theme	generated	301:309	arg1	nanoparticles					318:330	in situ generated silver nanoparticles	293:330	in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	293:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	0	31	theme	silver	136:141	arg1	nanoparticles					143:155	in situ generated silver nanoparticles	118:155	in situ generated silver nanoparticles for food packaging purpose	118:182	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	1	32	theme	one-step	205:212	arg1	preparation					214:224	a one-step preparation	203:224	a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	203:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	5	33	theme	film	875:878	arg1	transmittance					848:860	the light transmittance	838:860	the light transmittance of composite film	838:878	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	1	34	theme	silver	311:316	arg1	PVA/BRS/sAgNPs					333:346	PVA/BRS/sAgNPs	333:346	PVA/BRS/sAgNPs	333:346	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	1	34	theme	silver	311:316	arg1	nanoparticles					318:330	in situ generated silver nanoparticles	293:330	in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	293:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	0	35	theme	generated	126:134	arg1	nanoparticles					143:155	in situ generated silver nanoparticles	118:155	in situ generated silver nanoparticles for food packaging purpose	118:182	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	5	36	theme	light	842:846	arg1	transmittance					848:860	the light transmittance	838:860	the light transmittance of composite film	838:878	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	5	37	theme	composite	865:873	arg1	film					875:878	composite film	865:878	composite film	865:878	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	0	38	theme	rice	42:45	arg1	starch					47:52	eco-friendly boiled rice starch	22:52	eco-friendly boiled rice starch	22:52	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	0	39	dep	blended	54:60	arg1	bionanocomposite					80:95	bionanocomposite	80:95	blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose	54:182	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	2	40	theme	brown	440:444	arg1	color					446:450	dark brown color	435:450	dark brown color	435:450	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	0	41	theme	boiled	35:40	arg1	starch					47:52	eco-friendly boiled rice starch	22:52	eco-friendly boiled rice starch	22:52	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	8	42	theme	effective	1269:1277	arg1	material					1294:1301	cost effective food packaging material	1264:1301	cost effective food packaging material	1264:1301	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	2	43	theme	characteristic	459:472	arg1	peak					500:503	a characteristic surface plasmon resonance peak	457:503	a characteristic surface plasmon resonance peak at 439 nm	457:513	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	1	44	theme	polyvinyl	229:237	arg1	film					272:275	polyvinyl alcohol/boiled rice starch blend film	229:275	polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	229:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	7	45	theme	FTIR	1125:1128	arg1	analysis					1130:1137	FTIR analysis	1125:1137	FTIR analysis	1125:1137	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	0	46	theme	packaging	166:174	arg1	purpose					176:182	food packaging purpose	161:182	food packaging purpose	161:182	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	2	47	contain	have	430:433	arg1	bionanocomposite					401:416	The bionanocomposite	397:416	The bionanocomposite	397:416	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	2	47	contain	have	430:433	arg2	color					446:450	dark brown color	435:450	dark brown color	435:450	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	6	48	theme	superior	929:936	arg1	property					946:953	superior barrier property	929:953	superior barrier property	929:953	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	0	49	theme	starch	47:52	arg1	synthesis					9:17	One-step synthesis	0:17	One-step synthesis of eco-friendly boiled rice starch	0:52	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	0	50	theme	food	161:164	arg1	purpose					176:182	food packaging purpose	161:182	food packaging purpose	161:182	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	6	51	theme	PVA/BRS/sAgNPs	891:904	arg1	film					906:909	PVA/BRS/sAgNPs film	891:909	PVA/BRS/sAgNPs film	891:909	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	7	52	theme	visual	1090:1095	arg1	appearance					1097:1106	visual appearance	1090:1106	visual appearance	1090:1106	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	8	53	theme	PVA/BRS/sAgNPs	1176:1189	arg1	films					1191:1195	both the PVA/BRS and PVA/BRS/sAgNPs films	1155:1195	films	1191:1195	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	8	53	theme	PVA/BRS/sAgNPs	1176:1189	arg1	biodegradable					1210:1222	biodegradable	1210:1222	biodegradable	1210:1222	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	1	54	theme	alcohol/boiled	239:252	arg1	film					272:275	polyvinyl alcohol/boiled rice starch blend film	229:275	polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	229:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	3	55	theme	interactions	580:591	arg1	presence					559:566	the presence	555:566	the presence of physical interactions among the components PVA, BRS and sAgNPs	555:632	Further characterization has confirmed the presence of physical interactions among the components PVA, BRS and sAgNPs.
31362023	8	56	theme	promising	1239:1247	arg1	application					1249:1259	promising application	1239:1259	promising application	1239:1259	Interestingly, both the PVA/BRS and PVA/BRS/sAgNPs films proved to be biodegradable and hence have promising application as cost effective food packaging material with the latter having marked antimicrobial property.
31362023	7	57	theme	burial	1058:1063	arg1	test					1065:1068	indoor soil burial test	1046:1068	indoor soil burial test	1046:1068	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	3	58	theme	Further	516:522	arg1	characterization					524:539	Further characterization	516:539	Further characterization	516:539	Further characterization has confirmed the presence of physical interactions among the components PVA, BRS and sAgNPs.
31362023	1	59	theme	rice	254:257	arg1	film					272:275	polyvinyl alcohol/boiled rice starch blend film	229:275	polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	229:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	5	60	theme	sAgNPs	802:807	arg1	Presence					767:774	Presence	767:774	Presence of boiled rice starch and sAgNPs	767:807	Presence of boiled rice starch and sAgNPs were also found to influence the light transmittance of composite film.
31362023	2	61	theme	dark	435:438	arg1	color					446:450	dark brown color	435:450	dark brown color	435:450	The bionanocomposite appeared to have dark brown color with a characteristic surface plasmon resonance peak at 439 nm.
31362023	6	62	contain	have	924:927	arg2	property					946:953	superior barrier property	929:953	superior barrier property	929:953	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	6	62	contain	have	924:927	arg1	film					906:909	PVA/BRS/sAgNPs film	891:909	PVA/BRS/sAgNPs film	891:909	Moreover, PVA/BRS/sAgNPs film was found to have superior barrier property against environmental microorganisms.
31362023	3	63	dep	components	603:612	arg1	PVA					614:616	PVA	614:616	the components PVA	599:616	Further characterization has confirmed the presence of physical interactions among the components PVA, BRS and sAgNPs.
31362023	1	64	theme	starch	259:264	arg1	film					272:275	polyvinyl alcohol/boiled rice starch blend film	229:275	polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	229:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
31362023	0	65	theme	polyvinyl	62:70	arg1	alcohol					72:78	polyvinyl alcohol	62:78	polyvinyl alcohol	62:78	One-step synthesis of eco-friendly boiled rice starch blended polyvinyl alcohol bionanocomposite films decorated with in situ generated silver nanoparticles for food packaging purpose.
31362023	4	66	theme	control	651:657	arg1	PVA					659:661	the control PVA	647:661	the control PVA	647:661	Compared to the control PVA, the nanocomposites showed improved mechanical and optical properties with decreased water sensitivity.
31362023	7	67	theme	indoor	1046:1051	arg1	test					1065:1068	indoor soil burial test	1046:1068	indoor soil burial test	1046:1068	Biodegradation of the composite films was studied by indoor soil burial test and was assessed by visual appearance, weight loss and FTIR analysis.
31362023	1	68	theme	blend	266:270	arg1	film					272:275	polyvinyl alcohol/boiled rice starch blend film	229:275	polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation	229:394	The study reports a one-step preparation of polyvinyl alcohol/boiled rice starch blend film fabricated with in situ generated silver nanoparticles (PVA/BRS/sAgNPs) formed in the presence of sunlight irradiation.
30218734	4	0	theme	poly	709:712	arg1	grafting					697:704	grafting	697:704	grafting of poly(methyl methacrylate) (PMMA)	697:740	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	6	1	from	15days	1083:1088	arg1	intervals					1068:1076	different time intervals	1053:1076	different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability	1053:1165	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	2	2	theme	biopolymer-based	325:340	arg1	material					363:370	a novel biopolymer-based hybrid nanocomposite material	317:370	a novel biopolymer-based hybrid nanocomposite material	317:370	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	4	3	from	atmosphere	856:865	arg1	strength					814:821	the mechanical strength	799:821	the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator	799:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	2	4	dep	assess	468:473	arg1	proposed					478:485	proposed	478:485	proposed	478:485	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	8	5	located	found	1383:1387	arg1	pH-4					1392:1395	pH-4	1392:1395	pH-4	1392:1395	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	8	5	located	found	1383:1387	arg2	result					1351:1356	The optimum result	1339:1356	The optimum result for chromium removal	1339:1377	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	2	6	theme	novel	319:323	arg1	material					363:370	a novel biopolymer-based hybrid nanocomposite material	317:370	a novel biopolymer-based hybrid nanocomposite material	317:370	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	1	7	theme	water	250:254	arg1	bodies					256:261	the water bodies	246:261	the water bodies leading to water remediation	246:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	4	8	theme	ammonium	874:881	arg1	persulphate					883:893	ammonium persulphate	874:893	ammonium persulphate	874:893	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	3	9	theme	biodegradable	521:533	arg1	material					549:556	a biocompatible, biodegradable and non-toxic material	504:556	a biocompatible, biodegradable and non-toxic material	504:556	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	3	9	theme	biodegradable	521:533	arg1	Chitosan					492:499	Chitosan	492:499	Chitosan	492:499	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	4	10	theme	silica	758:763	arg1	gel					765:767	crosslinker silica gel	746:767	crosslinker silica gel	746:767	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	6	11	theme	good	1145:1148	arg1	biodegradability					1150:1165	good biodegradability	1145:1165	good biodegradability	1145:1165	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	8	12	theme	wastewater	1461:1470	arg1	Cr					1483:1484	wastewater containing Cr	1461:1484	wastewater containing Cr (VI)	1461:1489	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	4	13	theme	crosslinker	746:756	arg1	gel					765:767	crosslinker silica gel	746:767	crosslinker silica gel	746:767	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	7	14	theme	contact	1228:1234	arg1	time					1236:1239	the contact time	1224:1239	the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite	1224:1336	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	3	15	theme	biocompatible	506:518	arg1	material					549:556	a biocompatible, biodegradable and non-toxic material	504:556	a biocompatible, biodegradable and non-toxic material	504:556	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	3	15	theme	biocompatible	506:518	arg1	Chitosan					492:499	Chitosan	492:499	Chitosan	492:499	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	0	16	theme	emulsifier-free	90:104	arg1	polymerisation					115:128	emulsifier-free emulsion polymerisation	90:128	emulsifier-free emulsion polymerisation	90:128	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	4	17	from	nanocomposite	830:842	arg1	atmosphere					856:865	nitrogen atmosphere	847:865	nitrogen atmosphere taking ammonium persulphate as initiator	847:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	6	18	theme	samples	1030:1036	arg1	biodegradability					1006:1021	the biodegradability	1002:1021	the biodegradability of the samples	1002:1036	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	3	19	theme	non-toxic	539:547	arg1	material					549:556	a biocompatible, biodegradable and non-toxic material	504:556	a biocompatible, biodegradable and non-toxic material	504:556	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	3	19	theme	non-toxic	539:547	arg1	Chitosan					492:499	Chitosan	492:499	Chitosan	492:499	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	6	20	theme	time	1063:1066	arg1	intervals					1068:1076	different time intervals	1053:1076	different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability	1053:1165	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	2	21	theme	assess	468:473	arg1	use					487:489	its assess as proposed use	464:489	its assess as proposed use	464:489	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	8	22	theme	Cr	1483:1484	arg1	treatment					1448:1456	the treatment	1444:1456	the treatment of wastewater containing Cr (VI)	1444:1489	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	0	23	dep	bionanocomposite	60:75	arg1	prepared					77:84	prepared	77:84	bionanocomposite prepared via emulsifier-free emulsion polymerisation	60:128	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	4	24	theme	chitosan	666:673	arg1	modification					635:646	The chemical modification	622:646	The chemical modification of the biopolymer chitosan	622:673	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	0	25	theme	toxic	7:11	arg1	/silica					52:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	8	26	theme	containing	1472:1481	arg1	Cr					1483:1484	wastewater containing Cr	1461:1484	wastewater containing Cr (VI)	1461:1489	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	0	27	dep	/silica	52:58	arg1	VI					17:18	VI	17:18	VI	17:18	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	4	28	theme	nanocomposite	830:842	arg1	strength					814:821	the mechanical strength	799:821	the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator	799:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	2	29	theme	Cr	307:308	arg1	Adsorption					293:302	Adsorption	293:302	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material	293:370	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	1	30	theme	desirable	182:190	arg1	properties					192:201	desirable properties	182:201	desirable properties	182:201	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	1	31	theme	water	274:278	arg1	remediation					280:290	water remediation	274:290	water remediation	274:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	1	32	from	bodies	256:261	arg1	adsorption					230:239	heavy metal ion adsorption	214:239	heavy metal ion adsorption from the water bodies leading to water remediation	214:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	4	33	theme	nitrogen	847:854	arg1	atmosphere					856:865	nitrogen atmosphere	847:865	nitrogen atmosphere taking ammonium persulphate as initiator	847:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	8	34	theme	optimum	1343:1349	arg1	result					1351:1356	The optimum result	1339:1356	The optimum result for chromium removal	1339:1377	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	2	35	theme	polymerisation	422:435	arg1	technique					437:445	emulsifier-free emulsion polymerisation technique	397:445	emulsifier-free emulsion polymerisation technique	397:445	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	0	36	theme	Cr	13:14	arg1	/silica					52:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	0	37	theme	emulsion	106:113	arg1	polymerisation					115:128	emulsifier-free emulsion polymerisation	90:128	emulsifier-free emulsion polymerisation	90:128	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	6	38	theme	produced	1112:1119	arg1	nanocomposite					1121:1133	the so produced nanocomposite	1105:1133	the so produced nanocomposite exhibited good biodegradability	1105:1165	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	2	39	theme	emulsion	413:420	arg1	technique					437:445	emulsifier-free emulsion polymerisation technique	397:445	emulsifier-free emulsion polymerisation technique	397:445	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	4	40	theme	biopolymer	655:664	arg1	chitosan					666:673	the biopolymer chitosan	651:673	the biopolymer chitosan	651:673	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	7	41	theme	composite	1328:1336	arg1	pH					1288:1289	pH	1288:1289	pH of the solution	1288:1305	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	composite					1328:1336	the composite	1324:1336	the composite	1324:1336	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	solution					1298:1305	the solution	1294:1305	the solution	1294:1305	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	nanocomposite					1273:1285	the nanocomposite	1269:1285	the nanocomposite	1269:1285	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	adsorbate					1253:1261	the adsorbate	1249:1261	the adsorbate (Cr)	1249:1266	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	doses					1315:1319	the doses	1311:1319	the doses of the composite	1311:1336	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	41	theme	composite	1328:1336	arg1	Cr					1264:1265	Cr	1264:1265	Cr	1264:1265	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	2	42	theme	emulsifier-free	397:411	arg1	emulsion					413:420	emulsifier-free emulsion	397:420	emulsifier-free emulsion polymerisation technique	397:445	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	3	43	theme	research	607:614	arg1	work					616:619	this research work	602:619	this research work	602:619	Chitosan is a biocompatible, biodegradable and non-toxic material, hence this biopolymer has been selected in this research work.
30218734	4	44	from	strength	814:821	arg1	atmosphere					856:865	nitrogen atmosphere	847:865	nitrogen atmosphere taking ammonium persulphate as initiator	847:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	1	45	theme	heavy	214:218	arg1	adsorption					230:239	heavy metal ion adsorption	214:239	heavy metal ion adsorption from the water bodies leading to water remediation	214:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	0	46	theme	by	32:33	arg1	/silica					52:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	4	47	theme	methyl	714:719	arg1	poly					709:712	poly	709:712	poly(methyl methacrylate) (PMMA)	709:740	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	4	47	theme	methyl	714:719	arg1	methacrylate					721:732	methyl methacrylate	714:732	methyl methacrylate	714:732	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	0	48	theme	adsorption	21:30	arg1	/silica					52:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	4	49	theme	mechanical	803:812	arg1	strength					814:821	the mechanical strength	799:821	the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator	799:906	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	5	50	theme	resulting	913:921	arg1	samples					933:939	The resulting composite samples	909:939	The resulting composite samples	909:939	The resulting composite samples were characterized by using XRD, FTIR, SEM and TEM.
30218734	5	51	theme	composite	923:931	arg1	samples					933:939	The resulting composite samples	909:939	The resulting composite samples	909:939	The resulting composite samples were characterized by using XRD, FTIR, SEM and TEM.
30218734	1	52	theme	metal	220:224	arg1	adsorption					230:239	heavy metal ion adsorption	214:239	heavy metal ion adsorption from the water bodies leading to water remediation	214:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	8	53	theme	chromium	1362:1369	arg1	removal					1371:1377	chromium removal	1362:1377	chromium removal	1362:1377	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	2	54	dep	Cr	307:308	arg1	VI					310:311	VI	310:311	VI	310:311	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	1	55	theme	ion	226:228	arg1	adsorption					230:239	heavy metal ion adsorption	214:239	heavy metal ion adsorption from the water bodies leading to water remediation	214:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	0	56	theme	chitosan-g-PMMA	36:50	arg1	/silica					52:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica	0:58	Highly toxic Cr (VI) adsorption by (chitosan-g-PMMA)/silica bionanocomposite prepared via emulsifier-free emulsion polymerisation.
30218734	7	57	theme	solution	1298:1305	arg1	pH					1288:1289	pH	1288:1289	pH of the solution	1288:1305	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	composite					1328:1336	the composite	1324:1336	the composite	1324:1336	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	solution					1298:1305	the solution	1294:1305	the solution	1294:1305	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	nanocomposite					1273:1285	the nanocomposite	1269:1285	the nanocomposite	1269:1285	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	adsorbate					1253:1261	the adsorbate	1249:1261	the adsorbate (Cr)	1249:1266	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	doses					1315:1319	the doses	1311:1319	the doses of the composite	1311:1336	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	7	57	theme	solution	1298:1305	arg1	Cr					1264:1265	Cr	1264:1265	Cr	1264:1265	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	1	58	theme	properties	192:201	arg1	composites					168:177	some composites	163:177	some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation	163:290	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	1	58	theme	properties	192:201	arg1	properties					192:201	desirable properties	182:201	desirable properties	182:201	This work focuses on developing some composites of desirable properties mainly for heavy metal ion adsorption from the water bodies leading to water remediation.
30218734	2	59	theme	nanocomposite	349:361	arg1	material					363:370	a novel biopolymer-based hybrid nanocomposite material	317:370	a novel biopolymer-based hybrid nanocomposite material	317:370	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
30218734	6	60	theme	different	1053:1061	arg1	intervals					1068:1076	different time intervals	1053:1076	different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability	1053:1165	Further, the biodegradability of the samples was studied at different time intervals from 15days to 6months and the so produced nanocomposite exhibited good biodegradability.
30218734	4	61	theme	chemical	626:633	arg1	modification					635:646	The chemical modification	622:646	The chemical modification of the biopolymer chitosan	622:673	The chemical modification of the biopolymer chitosan has been achieved via grafting of poly(methyl methacrylate) (PMMA) and crosslinker silica gel was added in order to improve the mechanical strength of the nanocomposite in nitrogen atmosphere taking ammonium persulphate as initiator.
30218734	8	62	dep	Cr	1483:1484	arg1	VI					1487:1488	VI	1487:1488	VI	1487:1488	The optimum result for chromium removal was found at pH-4 and this result showed its use efficiently for the treatment of wastewater containing Cr (VI).
30218734	7	63	theme	Chromium	1182:1189	arg1	Adsorption					1168:1177	Adsorption	1168:1177	Adsorption of Chromium (Cr)	1168:1194	Adsorption of Chromium (Cr) was investigated by varying the contact time between the adsorbate (Cr), the nanocomposite, pH of the solution and the doses of the composite.
30218734	2	64	theme	hybrid	342:347	arg1	material					363:370	a novel biopolymer-based hybrid nanocomposite material	317:370	a novel biopolymer-based hybrid nanocomposite material	317:370	Adsorption of Cr(VI) by a novel biopolymer-based hybrid nanocomposite material has been synthesized via emulsifier-free emulsion polymerisation technique and investigated its assess as proposed use.
31883897	0	0	theme	Coleoptera	78:87	arg1	species					104:110	four Coleoptera and Orthoptera species	73:110	four Coleoptera and Orthoptera species	73:110	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	1	1	theme	order	284:288	arg1	Orthoptera					290:299	order Orthoptera	284:299	order Orthoptera	284:299	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	4	2	theme	Thermogravimetric	680:696	arg1	Analysis					698:705	Thermogravimetric Analysis	680:705	Thermogravimetric Analysis (TGA)	680:711	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	4	2	theme	Thermogravimetric	680:696	arg1	TGA					708:710	TGA	708:710	TGA	708:710	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	1	3	dep	Linnaeus	390:397	arg1	1758					400:403	1758	400:403	1758	400:403	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	3	4	theme	chitins	551:557	arg1	Yields					541:546	Yields	541:546	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	541:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	3	4	theme	chitins	551:557	arg1	%					641:641	11.3%	637:641	11.3%	637:641	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	0	5	theme	Orthoptera	93:102	arg1	species					104:110	four Coleoptera and Orthoptera species	73:110	four Coleoptera and Orthoptera species	73:110	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	1	6	dep	Linnaeus	249:256	arg1	1758					259:262	1758	259:262	1758	259:262	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	7	theme	Gryllotalpa	365:375	arg1	gryllotalpa					377:387	Gryllotalpa gryllotalpa	365:387	Gryllotalpa gryllotalpa (Linnaeus, 1758)	365:404	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	7	theme	Gryllotalpa	365:375	arg1	Linnaeus					390:397	Linnaeus	390:397	Linnaeus	390:397	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Lucanus					183:189	Lucanus	183:189	Lucanus	183:189	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Scarabaeidae					266:277	Scarabaeidae	266:277	Scarabaeidae	266:277	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Lucanidae					216:224	Lucanidae	216:224	Lucanidae	216:224	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Gryllotalpidae					407:420	Gryllotalpidae	407:420	Gryllotalpidae	407:420	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Linnaeus					249:256	Linnaeus	249:256	Linnaeus	249:256	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Tettigonidae					347:358	Tettigonidae	347:358	Tettigonidae	347:358	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	Linnaeus					199:206	Linnaeus	199:206	Linnaeus	199:206	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	8	dep	extracted	126:134	arg1	fullo					242:246	fullo	242:246	fullo	242:246	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	4	9	theme	chitin	754:759	arg1	samples					761:767	chitin samples	754:767	chitin samples from 614 °C to 748 °C	754:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	9	10	theme	promising	1399:1407	arg1	sources					1428:1434	promising alternative chitin sources	1399:1434	promising alternative chitin sources without negative effects on biodiversity	1399:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	9	10	theme	promising	1399:1407	arg1	adults					1363:1368	adults	1363:1368	adults of them	1363:1376	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	9	11	from	effects	1453:1459	arg1	biodiversity					1464:1475	biodiversity	1464:1475	biodiversity	1464:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	3	12	from	chitins	551:557	arg1	basis					566:570	dry basis	562:570	dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	562:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	5	13	theme	80.6	891:894	arg1	%					895:895	%	895:895	%	895:895	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	9	14	theme	alternative	1409:1419	arg1	sources					1428:1434	promising alternative chitin sources	1399:1434	promising alternative chitin sources without negative effects on biodiversity	1399:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	9	14	theme	alternative	1409:1419	arg1	adults					1363:1368	adults	1363:1368	adults of them	1363:1376	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	2	15	dep	Transform	452:460	arg1	Infrared					462:469	Infrared	462:469	Transform Infrared Spectrometry (FT-IR)	452:490	Fourier Transform Infrared Spectrometry (FT-IR) confirms that isolation of chitin is successful.
31883897	8	16	theme	FT-IR	1302:1306	arg1	data					1308:1311	FT-IR data	1302:1311	FT-IR data	1302:1311	It is detected that examined insects have α-chitin form from XRD and FT-IR data.
31883897	9	17	theme	chitin	1421:1426	arg1	sources					1428:1434	promising alternative chitin sources	1399:1434	promising alternative chitin sources without negative effects on biodiversity	1399:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	9	17	theme	chitin	1421:1426	arg1	adults					1363:1368	adults	1363:1368	adults of them	1363:1376	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	1	18	theme	Coleoptera	171:180	arg1	species					154:160	large insect species	141:160	large insect species of order Coleoptera	141:180	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	4	19	theme	samples	761:767	arg1	stability					741:749	thermal stability	733:749	thermal stability of chitin samples from 614 °C to 748 °C	733:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	3	20	from	G.	598:599	arg1	basis					566:570	dry basis	562:570	dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	562:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	2	21	theme	chitin	519:524	arg1	isolation					506:514	isolation	506:514	isolation of chitin	506:524	Fourier Transform Infrared Spectrometry (FT-IR) confirms that isolation of chitin is successful.
31883897	5	22	theme	crystallinity	858:870	arg1	percent					878:884	a crystallinity index percent	856:884	a crystallinity index percent	856:884	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	8	23	theme	examined	1253:1260	arg1	insects					1262:1268	examined insects	1253:1268	examined insects	1253:1268	It is detected that examined insects have α-chitin form from XRD and FT-IR data.
31883897	4	24	theme	thermal	733:739	arg1	stability					741:749	thermal stability	733:749	thermal stability of chitin samples from 614 °C to 748 °C	733:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	7	25	theme	nitrogen	1164:1171	arg1	percent					1173:1179	nitrogen percent	1164:1179	nitrogen percent	1164:1179	Elemental analysis has been applied to determine the elemental composition of chitin and nitrogen percent was relatively low for all specimens than expected.
31883897	1	26	theme	sureyai	326:332	arg1	Ünal					334:337	sureyai Ünal	326:337	Bradyporus (Callimenus) sureyai Ünal	302:337	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	6	27	theme	Electron	916:923	arg1	SEM					937:939	SEM	937:939	SEM	937:939	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	6	27	theme	Electron	916:923	arg1	Microscope					925:934	Scanning Electron Microscope	907:934	Scanning Electron Microscope (SEM)	907:940	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	7	28	theme	Elemental	1075:1083	arg1	analysis					1085:1092	Elemental analysis	1075:1092	Elemental analysis	1075:1092	Elemental analysis has been applied to determine the elemental composition of chitin and nitrogen percent was relatively low for all specimens than expected.
31883897	6	29	theme	porous	1011:1016	arg1	scales					1054:1059	fibrous and porous for all species and changes from nm scales	999:1059	fibrous and porous for all species and changes from nm scales to μm scales	999:1072	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	4	30	from	stability	741:749	arg1	748 °C					784:789	748 °C	784:789	748 °C	784:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	7	31	theme	chitin	1153:1158	arg1	composition					1138:1148	the elemental composition	1124:1148	the elemental composition of chitin	1124:1158	Elemental analysis has been applied to determine the elemental composition of chitin and nitrogen percent was relatively low for all specimens than expected.
31883897	8	32	contain	have	1270:1273	arg1	insects					1262:1268	examined insects	1253:1268	examined insects	1253:1268	It is detected that examined insects have α-chitin form from XRD and FT-IR data.
31883897	8	32	contain	have	1270:1273	arg2	form					1284:1287	α-chitin form	1275:1287	α-chitin form	1275:1287	It is detected that examined insects have α-chitin form from XRD and FT-IR data.
31883897	4	33	theme	small	798:802	arg1	percent					804:810	a small percent	796:810	a small percent of ash	796:817	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	5	34	dep	%	895:895	arg1	to					897:898	to	897:898	to	897:898	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	4	35	theme	stability	741:749	arg1	variety					722:728	a variety	720:728	a variety of thermal stability of chitin samples from 614 °C to 748 °C	720:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	4	35	theme	stability	741:749	arg1	stability					741:749	thermal stability	733:749	thermal stability of chitin samples from 614 °C to 748 °C	733:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	5	36	theme	index	872:876	arg1	percent					878:884	a crystallinity index percent	856:884	a crystallinity index percent	856:884	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	1	37	dep	Lucanus	183:189	arg1	cervus					191:196	Lucanus cervus	183:196	Lucanus cervus (Linnaeus, 1758) (Lucanidae)	183:225	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	4	38	theme	ash	815:817	arg1	percent					804:810	a small percent	796:810	a small percent of ash	796:817	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	2	39	dep	Fourier	444:450	arg1	Transform					452:460	Transform	452:460	Transform Infrared Spectrometry (FT-IR)	452:490	Fourier Transform Infrared Spectrometry (FT-IR) confirms that isolation of chitin is successful.
31883897	0	40	from	large	60:64	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	0	40	from	large	60:64	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	0	40	from	large	60:64	arg1	comparison					33:42	comparison	33:42	comparison	33:42	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	5	41	theme	diffraction	826:836	arg1	data					844:847	X-ray diffraction (XRD) data	820:847	X-ray diffraction (XRD) data	820:847	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	6	42	theme	Scanning	907:914	arg1	SEM					937:939	SEM	937:939	SEM	937:939	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	6	42	theme	Scanning	907:914	arg1	Microscope					925:934	Scanning Electron Microscope	907:934	Scanning Electron Microscope (SEM)	907:940	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	0	43	theme	chitins	47:53	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	0	43	theme	chitins	47:53	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	0	43	theme	chitins	47:53	arg1	comparison					33:42	comparison	33:42	comparison	33:42	Extraction, characterization and comparison of chitins from large bodied four Coleoptera and Orthoptera species.
31883897	1	44	dep	Bradyporus	302:311	arg1	Ünal					334:337	sureyai Ünal	326:337	Bradyporus (Callimenus) sureyai Ünal	302:337	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	9	45	theme	negative	1444:1451	arg1	effects					1453:1459	negative effects	1444:1459	negative effects on biodiversity	1444:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	6	46	theme	fibrous	999:1005	arg1	scales					1054:1059	fibrous and porous for all species and changes from nm scales	999:1059	fibrous and porous for all species and changes from nm scales to μm scales	999:1072	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	3	47	from	Yields	541:546	arg1	basis					566:570	dry basis	562:570	dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	562:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	4	48	dep	748 °C	784:789	arg1	to					781:782	to	781:782	to	781:782	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	1	49	dep	fullo	242:246	arg1	2011					340:343	2011	340:343	2011	340:343	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	49	dep	fullo	242:246	arg1	Callimenus					314:323	Callimenus	314:323	Callimenus	314:323	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	49	dep	fullo	242:246	arg1	Bradyporus					302:311	Bradyporus	302:311	Bradyporus	302:311	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	50	dep	Linnaeus	199:206	arg1	1758					209:212	1758	209:212	1758	209:212	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	5	51	theme	X-ray	820:824	arg1	XRD					839:841	XRD	839:841	XRD	839:841	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	5	51	theme	X-ray	820:824	arg1	diffraction					826:836	X-ray diffraction	820:836	X-ray diffraction (XRD) data	820:847	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	6	52	from	nm	1051:1052	arg1	changes					1038:1044	changes	1038:1044	changes	1038:1044	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	6	52	from	nm	1051:1052	arg1	species					1026:1032	all species	1022:1032	all species	1022:1032	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	6	53	theme	μm	1064:1065	arg1	scales					1067:1072	μm scales	1064:1072	μm scales	1064:1072	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	3	54	theme	dry	562:564	arg1	basis					566:570	dry basis	562:570	dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	562:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	7	55	theme	elemental	1128:1136	arg1	composition					1138:1148	the elemental composition	1124:1148	the elemental composition of chitin	1124:1158	Elemental analysis has been applied to determine the elemental composition of chitin and nitrogen percent was relatively low for all specimens than expected.
31883897	8	56	theme	α-chitin	1275:1282	arg1	form					1284:1287	α-chitin form	1275:1287	α-chitin form	1275:1287	It is detected that examined insects have α-chitin form from XRD and FT-IR data.
31883897	9	57	theme	them	1373:1376	arg1	sources					1428:1434	promising alternative chitin sources	1399:1434	promising alternative chitin sources without negative effects on biodiversity	1399:1475	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	9	57	theme	them	1373:1376	arg1	adults					1363:1368	adults	1363:1368	adults of them	1363:1376	If these species can be grown in the laboratory, adults of them could be accepted as promising alternative chitin sources without negative effects on biodiversity.
31883897	1	58	theme	large	141:145	arg1	species					154:160	large insect species	141:160	large insect species of order Coleoptera	141:180	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	4	59	from	748 °C	784:789	arg1	stability					741:749	thermal stability	733:749	thermal stability of chitin samples from 614 °C to 748 °C	733:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	4	59	from	748 °C	784:789	arg1	samples					761:767	chitin samples	754:767	chitin samples from 614 °C to 748 °C	754:789	Thermogravimetric Analysis (TGA) showed a variety of thermal stability of chitin samples from 614 °C to 748 °C with a small percent of ash.
31883897	3	60	from	basis	566:570	arg1	Yields					541:546	Yields	541:546	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai	541:631	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	3	60	from	basis	566:570	arg1	%					641:641	11.3%	637:641	11.3%	637:641	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	5	61	theme	85.2	900:903	arg1	%					895:895	%	895:895	%	895:895	X-ray diffraction (XRD) data showed a crystallinity index percent from 80.6% to 85.2%.
31883897	6	62	theme	surface	959:965	arg1	characterization					967:982	surface characterization	959:982	surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales	959:1072	Scanning Electron Microscope (SEM) was examined for surface characterization determining as fibrous and porous for all species and changes from nm scales to μm scales.
31883897	1	63	theme	first	432:436	arg1	time					438:441	the first time	428:441	the first time	428:441	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	1	64	theme	insect	147:152	arg1	species					154:160	large insect species	141:160	large insect species of order Coleoptera	141:180	Chitins were extracted from large insect species of order Coleoptera (Lucanus cervus (Linnaeus, 1758) (Lucanidae) and Polyphylla fullo (Linnaeus, 1758) (Scarabaeidae) and order Orthoptera (Bradyporus (Callimenus) sureyai Ünal, 2011) (Tettigonidae) and Gryllotalpa gryllotalpa (Linnaeus, 1758) (Gryllotalpidae)) for the first time.
31883897	3	65	dep	G.	598:599	arg1	B					617:617	B	617:617	B	617:617	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
31883897	3	65	dep	G.	598:599	arg1	gryllotalpa					601:611	gryllotalpa	601:611	gryllotalpa	601:611	Yields of chitins on dry basis from P. fullo, L. cervus, G. gryllotalpa and B. (C.) sureyai are 11.3%, 10.9%, 10.1% and 9.8% respectively.
29456010	0	0	from	characterization	15:30	arg1	films					108:112	edible films	101:112	edible films	101:112	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	5	1	theme	"	589:589	arg1	structures					608:617	"C" type crystalline structures	587:617	"C" type crystalline structures	587:617	The X-ray diffraction showed "C" type crystalline structures, while thermogram showed Tg around of 118 and 120 °C.
29456010	0	2	theme	starch	71:76	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	0	2	theme	starch	71:76	arg1	application					86:96	its application	82:96	its application in edible films	82:112	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	0	2	theme	starch	71:76	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	5	3	theme	type	591:594	arg1	structures					608:617	"C" type crystalline structures	587:617	"C" type crystalline structures	587:617	The X-ray diffraction showed "C" type crystalline structures, while thermogram showed Tg around of 118 and 120 °C.
29456010	4	4	theme	swelling	521:528	arg1	power					530:534	swelling power	521:534	swelling power	521:534	Low values were found for the swelling power and solubility index.
29456010	0	5	from	Extraction	0:9	arg1	films					108:112	edible films	101:112	edible films	101:112	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	5	6	theme	crystalline	596:606	arg1	structures					608:617	"C" type crystalline structures	587:617	"C" type crystalline structures	587:617	The X-ray diffraction showed "C" type crystalline structures, while thermogram showed Tg around of 118 and 120 °C.
29456010	6	7	theme	starch	736:741	arg1	loss					728:731	mass loss	723:731	mass loss of starch	723:741	The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C.
29456010	4	8	theme	solubility	540:549	arg1	index					551:555	solubility index	540:555	solubility index	540:555	Low values were found for the swelling power and solubility index.
29456010	9	9	theme	promising	1046:1054	arg1	arrowroot					1026:1034	arrowroot	1026:1034	arrowroot	1026:1034	Therefore, arrowroot is a very promising starch source for application in films.
29456010	9	9	theme	promising	1046:1054	arg1	source					1063:1068	a very promising starch source	1039:1068	a very promising starch source for application in films	1039:1093	Therefore, arrowroot is a very promising starch source for application in films.
29456010	0	10	theme	edible	101:106	arg1	films					108:112	edible films	101:112	edible films	101:112	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	3	11	theme	high	463:466	arg1	content					476:482	high amylose content	463:482	high amylose content (35%)	463:488	Arrowroot starch showed high amylose content (35%).
29456010	3	11	theme	high	463:466	arg1	%					487:487	35%	485:487	35%	485:487	Arrowroot starch showed high amylose content (35%).
29456010	8	12	theme	vapor	974:978	arg1	permeability					980:991	water vapor permeability	968:991	water vapor permeability	968:991	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	5	13	theme	X-ray	562:566	arg1	diffraction					568:578	The X-ray diffraction	558:578	The X-ray diffraction	558:578	The X-ray diffraction showed "C" type crystalline structures, while thermogram showed Tg around of 118 and 120 °C.
29456010	2	14	from	effects	206:212	arg1	properties					355:364	films properties	349:364	films properties	349:364	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	8	15	theme	water	968:972	arg1	permeability					980:991	water vapor permeability	968:991	water vapor permeability	968:991	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	6	16	theme	mass	723:726	arg1	loss					728:731	mass loss	723:731	mass loss of starch	723:741	The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C.
29456010	2	17	theme	films	349:353	arg1	properties					355:364	films properties	349:364	films properties	349:364	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	18	theme	glycerol	298:305	arg1	concentrations					280:293	concentrations	280:293	concentrations of glycerol (9.95-24.08%, mass versus starch mass)	280:344	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	18	theme	glycerol	298:305	arg1	concentrations					227:240	different concentrations	217:240	different concentrations of starch (2.59-5.41%, mass/mass)	217:274	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	1	19	theme	arrowroot	175:183	arg1	starch					185:190	arrowroot starch	175:190	arrowroot starch	175:190	This research work aimed extraction and characterization of arrowroot starch.
29456010	2	20	theme	composite	405:413	arg1	design					431:436	a rotational central composite 22 experimental design	384:436	a rotational central composite 22 experimental design	384:436	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	1	21	theme	starch	185:190	arg1	characterization					155:170	characterization	155:170	characterization	155:170	This research work aimed extraction and characterization of arrowroot starch.
29456010	1	21	theme	starch	185:190	arg1	extraction					140:149	extraction	140:149	extraction	140:149	This research work aimed extraction and characterization of arrowroot starch.
29456010	2	22	theme	central	397:403	arg1	design					431:436	a rotational central composite 22 experimental design	384:436	a rotational central composite 22 experimental design	384:436	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	6	23	theme	loss	728:731	arg1	%					718:718	40%	716:718	40% of mass loss of starch	716:741	The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C.
29456010	6	23	theme	loss	728:731	arg1	loss					728:731	mass loss	723:731	mass loss of starch	723:741	The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C.
29456010	2	24	theme	concentrations	280:293	arg1	effects					206:212	the effects	202:212	the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties	202:364	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	8	25	from	thickness	896:904	arg1	water					924:928	water	924:928	water of films	924:937	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	2	26	theme	rotational	386:395	arg1	design					431:436	a rotational central composite 22 experimental design	384:436	a rotational central composite 22 experimental design	384:436	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	8	27	theme	tensile	997:1003	arg1	strength					1005:1012	tensile strength	997:1012	tensile strength	997:1012	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	3	28	theme	Arrowroot	439:447	arg1	starch					449:454	Arrowroot starch	439:454	Arrowroot starch	439:454	Arrowroot starch showed high amylose content (35%).
29456010	2	29	theme	concentrations	227:240	arg1	effects					206:212	the effects	202:212	the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties	202:364	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	9	30	from	application	1074:1084	arg1	films					1089:1093	films	1089:1093	films	1089:1093	Therefore, arrowroot is a very promising starch source for application in films.
29456010	2	31	dep	concentrations	227:240	arg1	mass/mass					265:273	mass/mass	265:273	mass/mass	265:273	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	31	dep	concentrations	227:240	arg1	%					262:262	2.59-5.41%	253:262	2.59-5.41%	253:262	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	0	32	theme	arrowroot	35:43	arg1	starch					71:76	arrowroot (Maranta arundinaceae L.) starch	35:76	arrowroot (Maranta arundinaceae L.) starch	35:76	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	0	32	theme	arrowroot	35:43	arg1	Maranta					46:52	Maranta	46:52	Maranta	46:52	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	4	33	dep	power	530:534	arg1	the					517:519	the	517:519	the	517:519	Low values were found for the swelling power and solubility index.
29456010	0	34	dep	Maranta	46:52	arg1	L.					67:68	Maranta arundinaceae L.	46:68	Maranta arundinaceae L.	46:68	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	8	35	theme	glycerol	843:850	arg1	concentrations					852:865	glycerol concentrations	843:865	glycerol concentrations	843:865	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	2	36	dep	concentrations	280:293	arg1	%					318:318	9.95-24.08%	308:318	9.95-24.08%	308:318	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	36	dep	concentrations	280:293	arg1	mass					321:324	mass	321:324	mass	321:324	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	36	dep	concentrations	280:293	arg1	mass					340:343	starch mass	333:343	starch mass	333:343	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	37	theme	experimental	418:429	arg1	design					431:436	a rotational central composite 22 experimental design	384:436	a rotational central composite 22 experimental design	384:436	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	9	38	theme	starch	1056:1061	arg1	arrowroot					1026:1034	arrowroot	1026:1034	arrowroot	1026:1034	Therefore, arrowroot is a very promising starch source for application in films.
29456010	9	38	theme	starch	1056:1061	arg1	source					1063:1068	a very promising starch source	1039:1068	a very promising starch source for application in films	1039:1093	Therefore, arrowroot is a very promising starch source for application in films.
29456010	3	39	theme	amylose	468:474	arg1	content					476:482	high amylose content	463:482	high amylose content (35%)	463:488	Arrowroot starch showed high amylose content (35%).
29456010	3	39	theme	amylose	468:474	arg1	%					487:487	35%	485:487	35%	485:487	Arrowroot starch showed high amylose content (35%).
29456010	4	40	theme	Low	491:493	arg1	values					495:500	Low values	491:500	Low values	491:500	Low values were found for the swelling power and solubility index.
29456010	8	41	theme	films	933:937	arg1	water					924:928	water	924:928	water of films	924:937	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	2	42	theme	different	217:225	arg1	concentrations					227:240	different concentrations	217:240	different concentrations of starch (2.59-5.41%, mass/mass)	217:274	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	8	43	from	solubility	910:919	arg1	water					924:928	water	924:928	water of films	924:937	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	6	44	theme	thermogravimetric	677:693	arg1	analysis					695:702	The thermogravimetric analysis	673:702	The thermogravimetric analysis	673:702	The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C.
29456010	0	45	from	application	86:96	arg1	films					108:112	edible films	101:112	edible films	101:112	Extraction and characterization of arrowroot (Maranta arundinaceae L.) starch and its application in edible films.
29456010	1	46	theme	research	120:127	arg1	work					129:132	This research work	115:132	This research work	115:132	This research work aimed extraction and characterization of arrowroot starch.
29456010	2	47	theme	starch	245:250	arg1	concentrations					280:293	concentrations	280:293	concentrations of glycerol (9.95-24.08%, mass versus starch mass)	280:344	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	47	theme	starch	245:250	arg1	concentrations					227:240	different concentrations	217:240	different concentrations of starch (2.59-5.41%, mass/mass)	217:274	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	8	48	theme	significant	876:886	arg1	role					888:891	a significant role	874:891	a significant role	874:891	Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength.
29456010	2	49	theme	starch	333:338	arg1	%					318:318	9.95-24.08%	308:318	9.95-24.08%	308:318	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
29456010	2	49	theme	starch	333:338	arg1	mass					340:343	starch mass	333:343	starch mass	333:343	Besides, the effects of different concentrations of starch (2.59-5.41%, mass/mass) and concentrations of glycerol (9.95-24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design.
31622700	0	0	theme	stearic	86:92	arg1	acid					94:97	stearic acid	86:97	stearic acid	86:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	1	1	theme	strength	323:330	arg1	agent					332:336	strength agent	323:336	strength agent	323:336	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	4	2	theme	hydrophobic	817:827	arg1	properties					841:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	6	3	theme	enhancement	1414:1424	arg1	better					1446:1451	better	1446:1451	better	1446:1451	Overall, the enhancement effect of M-MCC was better than M-NCC.
31622700	6	3	theme	enhancement	1414:1424	arg1	effect					1426:1431	the enhancement effect	1410:1431	the enhancement effect of M-MCC	1410:1440	Overall, the enhancement effect of M-MCC was better than M-NCC.
31622700	5	4	theme	tensile	1178:1184	arg1	strength					1186:1193	the tensile strength	1174:1193	the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively	1174:1260	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	1	5	theme	stearic	241:247	arg1	acid					249:252	stearic acid	241:252	stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC)	241:318	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	5	6	contain	had	1105:1107	arg1	M-NCC					1099:1103	1.5% M-NCC	1094:1103	1.5% M-NCC	1094:1103	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	6	contain	had	1105:1107	arg1	M-MCC					1084:1088	0.5% M-MCC	1079:1088	0.5% M-MCC	1079:1088	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	6	contain	had	1105:1107	arg2	effect					1133:1138	the highest enhancement effect	1109:1138	the highest enhancement effect on mechanical properties	1109:1163	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	3	7	theme	stearic	647:653	arg1	acid					655:658	stearic acid	647:658	stearic acid	647:658	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	0	8	theme	strength	164:171	arg1	agent					173:177	strength agent	164:177	strength agent	164:177	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	1	9	theme	food	417:420	arg1	package					422:428	food package	417:428	food package in this work	417:441	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	1	10	from	package	422:428	arg1	work					438:441	this work	433:441	this work	433:441	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	5	11	theme	enhancement	1121:1131	arg1	effect					1133:1138	the highest enhancement effect	1109:1138	the highest enhancement effect on mechanical properties	1109:1163	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	2	12	theme	%	497:497	arg1	hydrolysis					515:524	98% phosphoric acid hydrolysis	495:524	98% phosphoric acid hydrolysis	495:524	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	4	13	theme	thermal	833:839	arg1	properties					841:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	2	14	theme	%	607:607	arg1	acid					618:621	65% sulfuric acid	605:621	65% sulfuric acid hydrolysis	605:632	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	1	15	theme	modified	254:261	arg1	cellulose					280:288	modified microcrystalline cellulose	254:288	modified microcrystalline cellulose	254:288	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	2	16	theme	98	495:496	arg1	%					497:497	%	497:497	%	497:497	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	0	17	with	films	75:79	arg1	acid					94:97	stearic acid	86:97	stearic acid	86:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	1	18	theme	microcrystalline	263:278	arg1	cellulose					280:288	modified microcrystalline cellulose	254:288	modified microcrystalline cellulose	254:288	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	4	19	theme	contact	920:926	arg1	meter					934:938	contact angle meter	920:938	contact angle meter	920:938	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	5	20	theme	hydrophobic	1036:1046	arg1	properties					1048:1057	mechanical and hydrophobic properties	1021:1057	mechanical and hydrophobic properties of composite films	1021:1076	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	4	21	theme	Physical	795:802	arg1	properties					841:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	2	22	theme	65	605:606	arg1	%					607:607	%	607:607	%	607:607	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	5	23	theme	mechanical	1021:1030	arg1	properties					1048:1057	mechanical and hydrophobic properties	1021:1057	mechanical and hydrophobic properties of composite films	1021:1076	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	0	24	theme	MCC	108:110	arg1	nanocellulose					146:158	nanocellulose	146:158	nanocellulose	146:158	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	0	24	theme	MCC	108:110	arg1	/NCC					140:143	MCC (microcrystalline cellulose)/NCC	108:143	MCC (microcrystalline cellulose)/NCC (nanocellulose)	108:159	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	1	25	theme	good	351:354	arg1	properties					383:392	good mechanical and hydrophobic properties	351:392	good mechanical and hydrophobic properties	351:392	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	3	26	theme	starch	781:786	arg1	films					788:792	M-NCC/cassava starch films	767:792	M-NCC/cassava starch films	767:792	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	5	27	theme	%	1297:1297	arg1	M-MCC					1299:1303	2% M-MCC	1296:1303	2% M-MCC	1296:1303	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	4	28	theme	universal	893:901	arg1	machine					911:917	electronic universal testing machine	882:917	electronic universal testing machine	882:917	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	0	29	theme	mechanical	9:18	arg1	properties					36:45	Enhanced mechanical and hydrophobic properties	0:45	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid	0:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	1	30	theme	mechanical	356:365	arg1	properties					383:392	good mechanical and hydrophobic properties	351:392	good mechanical and hydrophobic properties	351:392	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	4	31	theme	mechanical	805:814	arg1	properties					841:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties	795:850	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	5	32	theme	%	1097:1097	arg1	M-NCC					1099:1103	1.5% M-NCC	1094:1103	1.5% M-NCC	1094:1103	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	6	33	theme	M-MCC	1436:1440	arg1	better					1446:1451	better	1446:1451	better	1446:1451	Overall, the enhancement effect of M-MCC was better than M-NCC.
31622700	6	33	theme	M-MCC	1436:1440	arg1	effect					1426:1431	the enhancement effect	1410:1431	the enhancement effect of M-MCC	1410:1440	Overall, the enhancement effect of M-MCC was better than M-NCC.
31622700	0	34	theme	Enhanced	0:7	arg1	properties					36:45	Enhanced mechanical and hydrophobic properties	0:45	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid	0:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	2	35	from	nanocellulose	567:579	arg1	stirring					541:548	mechanical stirring	530:548	mechanical stirring at 600 r/min and nanocellulose (NCC)	530:585	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	5	36	theme	film	1353:1356	arg1	hydrophobicity					1331:1344	the hydrophobicity	1327:1344	the hydrophobicity of the film	1327:1356	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	37	theme	0.5	1309:1311	arg1	%					1312:1312	%	1312:1312	%	1312:1312	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	1	38	dep	using	235:239	arg1	shows					345:349	shows	345:349	shows good mechanical and hydrophobic properties	345:392	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	1	38	dep	using	235:239	arg1	candidate					403:411	a candidate	401:411	a candidate for food package in this work	401:441	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	0	39	theme	hydrophobic	24:34	arg1	properties					36:45	Enhanced mechanical and hydrophobic properties	0:45	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid	0:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	7	40	theme	films	1522:1526	arg1	stability					1509:1517	the thermal stability	1497:1517	the thermal stability of films	1497:1526	But when M-MCC/M-NCC was added, the thermal stability of films reduced.
31622700	5	41	from	effect	1133:1138	arg1	properties					1154:1163	mechanical properties	1143:1163	mechanical properties	1143:1163	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	42	theme	%	1312:1312	arg1	M-NCC					1314:1318	0.5% M-NCC	1309:1318	0.5% M-NCC	1309:1318	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	3	43	theme	M-NCC/cassava	767:779	arg1	films					788:792	M-NCC/cassava starch films	767:792	M-NCC/cassava starch films	767:792	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	2	44	theme	acid	618:621	arg1	hydrolysis					623:632	65% sulfuric acid hydrolysis	605:632	65% sulfuric acid hydrolysis	605:632	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	5	45	theme	films	1072:1076	arg1	properties					1048:1057	mechanical and hydrophobic properties	1021:1057	mechanical and hydrophobic properties of composite films	1021:1076	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	0	46	theme	microcrystalline	113:128	arg1	MCC					108:110	MCC	108:110	MCC (microcrystalline cellulose)/NCC (nanocellulose)	108:159	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	0	46	theme	microcrystalline	113:128	arg1	cellulose					130:138	microcrystalline cellulose	113:138	microcrystalline cellulose	113:138	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	4	47	theme	electronic	882:891	arg1	machine					911:917	electronic universal testing machine	882:917	electronic universal testing machine	882:917	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	5	48	theme	properties	1048:1057	arg1	enhancement					1006:1016	the enhancement	1002:1016	the enhancement of mechanical and hydrophobic properties of composite films	1002:1076	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	7	49	theme	thermal	1501:1507	arg1	stability					1509:1517	the thermal stability	1497:1517	the thermal stability of films	1497:1526	But when M-MCC/M-NCC was added, the thermal stability of films reduced.
31622700	1	50	theme	composite	190:198	arg1	films					215:219	The green composite cassava starch films	180:219	The green composite cassava starch films	180:219	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	3	51	theme	casting	687:693	arg1	method					695:700	the casting method	683:700	the casting method	683:700	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	2	52	theme	acid	510:513	arg1	hydrolysis					515:524	98% phosphoric acid hydrolysis	495:524	98% phosphoric acid hydrolysis	495:524	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	1	53	theme	hydrophobic	371:381	arg1	properties					383:392	good mechanical and hydrophobic properties	351:392	good mechanical and hydrophobic properties	351:392	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	1	54	theme	cassava	200:206	arg1	films					215:219	The green composite cassava starch films	180:219	The green composite cassava starch films	180:219	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	2	55	theme	Microcrystalline	444:459	arg1	MCC					472:474	MCC	472:474	MCC	472:474	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	2	55	theme	Microcrystalline	444:459	arg1	cellulose					461:469	Microcrystalline cellulose	444:469	Microcrystalline cellulose (MCC)	444:475	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	1	56	theme	/nanocellulose	297:310	arg1	acid					249:252	stearic acid	241:252	stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC)	241:318	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	2	57	theme	phosphoric	499:508	arg1	hydrolysis					515:524	98% phosphoric acid hydrolysis	495:524	98% phosphoric acid hydrolysis	495:524	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	1	58	theme	starch	208:213	arg1	films					215:219	The green composite cassava starch films	180:219	The green composite cassava starch films	180:219	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	0	59	theme	cassava	60:66	arg1	films					75:79	composite cassava starch films	50:79	composite cassava starch films with stearic acid	50:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	5	60	theme	%	1082:1082	arg1	M-MCC					1084:1088	0.5% M-MCC	1079:1088	0.5% M-MCC	1079:1088	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	4	61	theme	angle	928:932	arg1	meter					934:938	contact angle meter	920:938	contact angle meter	920:938	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	1	62	dep	/nanocellulose	297:310	arg1	cellulose					280:288	modified microcrystalline cellulose	254:288	modified microcrystalline cellulose	254:288	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	1	62	dep	/nanocellulose	297:310	arg1	M-NCC					313:317	M-NCC	313:317	M-NCC	313:317	The green composite cassava starch films were prepared using stearic acid modified microcrystalline cellulose (M-MCC)/nanocellulose (M-NCC) as strength agent, which shows good mechanical and hydrophobic properties and is a candidate for food package in this work.
31622700	0	63	theme	composite	50:58	arg1	films					75:79	composite cassava starch films	50:79	composite cassava starch films with stearic acid	50:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	5	64	theme	2	1296:1296	arg1	%					1297:1297	%	1297:1297	%	1297:1297	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	65	theme	cassava	1198:1204	arg1	film					1213:1216	cassava starch film	1198:1216	cassava starch film increased by 484.5% and 327.7% respectively	1198:1260	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	66	theme	1.5	1094:1096	arg1	%					1097:1097	%	1097:1097	%	1097:1097	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	3	67	used	used	706:709	arg2	method					695:700	the casting method	683:700	the casting method	683:700	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	2	68	theme	sulfuric	609:616	arg1	acid					618:621	65% sulfuric acid	605:621	65% sulfuric acid hydrolysis	605:632	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	5	69	theme	starch	1206:1211	arg1	film					1213:1216	cassava starch film	1198:1216	cassava starch film increased by 484.5% and 327.7% respectively	1198:1260	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	3	70	theme	composite	747:755	arg1	films					757:761	the M-MCC/cassava starch composite films	722:761	the M-MCC/cassava starch composite films	722:761	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	2	71	theme	mechanical	530:539	arg1	stirring					541:548	mechanical stirring	530:548	mechanical stirring at 600 r/min and nanocellulose (NCC)	530:585	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	5	72	theme	highest	1113:1119	arg1	effect					1133:1138	the highest enhancement effect	1109:1138	the highest enhancement effect on mechanical properties	1109:1163	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	4	73	theme	testing	903:909	arg1	machine					911:917	electronic universal testing machine	882:917	electronic universal testing machine	882:917	Physical, mechanical, hydrophobic and thermal properties were characterized using SEM, electronic universal testing machine, contact angle meter and TGA.
31622700	5	74	theme	film	1213:1216	arg1	strength					1186:1193	the tensile strength	1174:1193	the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively	1174:1260	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	0	75	theme	films	75:79	arg1	properties					36:45	Enhanced mechanical and hydrophobic properties	0:45	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid	0:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	3	76	theme	M-MCC/cassava	726:738	arg1	films					757:761	the M-MCC/cassava starch composite films	722:761	the M-MCC/cassava starch composite films	722:761	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31622700	0	77	theme	starch	68:73	arg1	films					75:79	composite cassava starch films	50:79	composite cassava starch films with stearic acid	50:97	Enhanced mechanical and hydrophobic properties of composite cassava starch films with stearic acid modified MCC (microcrystalline cellulose)/NCC (nanocellulose) as strength agent.
31622700	5	78	theme	composite	1062:1070	arg1	films					1072:1076	composite films	1062:1076	composite films	1062:1076	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	79	theme	hydrophobic	1269:1279	arg1	property					1281:1288	hydrophobic property	1269:1288	hydrophobic property	1269:1288	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	5	80	theme	mechanical	1143:1152	arg1	properties					1154:1163	mechanical properties	1143:1163	mechanical properties	1143:1163	The results showed that: M-MCC and M-NCC can lead to the enhancement of mechanical and hydrophobic properties of composite films; 0.5% M-MCC and 1.5% M-NCC had the highest enhancement effect on mechanical properties, leading the tensile strength of cassava starch film increased by 484.5% and 327.7% respectively; As to hydrophobic property, when 2% M-MCC and 0.5% M-NCC added, the hydrophobicity of the film increased by 65.0% and 30.3% respectively.
31622700	2	81	from	600 r/min	553:561	arg1	stirring					541:548	mechanical stirring	530:548	mechanical stirring at 600 r/min and nanocellulose (NCC)	530:585	Microcrystalline cellulose (MCC) was prepared with 98% phosphoric acid hydrolysis and mechanical stirring at 600 r/min and nanocellulose (NCC) was prepared with 65% sulfuric acid hydrolysis.
31622700	3	82	theme	starch	740:745	arg1	films					757:761	the M-MCC/cassava starch composite films	722:761	the M-MCC/cassava starch composite films	722:761	After using stearic acid to modify MCC and NCC, the casting method was used to prepare the M-MCC/cassava starch composite films and M-NCC/cassava starch films.
31385250	0	0	theme	Cr	106:107	arg1	adsorption					92:101	adsorption	92:101	adsorption of Cr(VI) and AYR	92:119	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	3	1	theme	area	693:696	arg1	BET					711:713	BET	711:713	BET	711:713	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	1	theme	area	693:696	arg1	measurement					698:708	Brunauer-Emmett-Teller surface area measurement	662:708	Brunauer-Emmett-Teller surface area measurement (BET)	662:714	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	1	2	with	oxide	147:151	arg1	polymers					169:176	polymers	169:176	polymers	169:176	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	1	2	with	oxide	147:151	arg1	biomass					158:164	biomass	158:164	biomass	158:164	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	1	3	theme	oxide	147:151	arg1	assembly					126:133	The assembly	122:133	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties	122:234	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	2	4	theme	graphene	326:333	arg1	composite					347:355	/reduced graphene oxide (rGO) composite	317:355	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	3	5	theme	Brunauer-Emmett-Teller	662:683	arg1	BET					711:713	BET	711:713	BET	711:713	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	5	theme	Brunauer-Emmett-Teller	662:683	arg1	measurement					698:708	Brunauer-Emmett-Teller surface area measurement	662:708	Brunauer-Emmett-Teller surface area measurement (BET)	662:714	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	4	6	theme	ionic	823:827	arg1	strength					829:836	ionic strength	823:836	ionic strength	823:836	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	3	7	theme	pore	491:494	arg1	size					496:499	pore size	491:499	pore size	491:499	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	8	theme	materials	516:524	arg1	composition					454:464	composition	454:464	composition	454:464	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	8	theme	materials	516:524	arg1	properties					475:484	surface properties	467:484	surface properties	467:484	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	8	theme	materials	516:524	arg1	morphology					442:451	The morphology	438:451	The morphology	438:451	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	8	theme	materials	516:524	arg1	size					496:499	pore size	491:499	pore size	491:499	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	1	9	theme	research	249:256	arg1	hotspot					258:264	a research hotspot	247:264	a research hotspot	247:264	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	6	10	theme	excellent	1065:1073	arg1	flexibility					1075:1085	excellent flexibility	1065:1085	excellent flexibility	1065:1085	Besides, composite hydrogel also exhibits excellent flexibility and good repeatability, which confirms its great potential as an adsorbent to remove pollutants in the water environment.
31385250	2	11	theme	oxide	335:339	arg1	composite					347:355	/reduced graphene oxide (rGO) composite	317:355	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	3	12	theme	surface	467:473	arg1	properties					475:484	surface properties	467:484	surface properties	467:484	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	6	13	theme	good	1091:1094	arg1	repeatability					1096:1108	good repeatability	1091:1108	good repeatability	1091:1108	Besides, composite hydrogel also exhibits excellent flexibility and good repeatability, which confirms its great potential as an adsorbent to remove pollutants in the water environment.
31385250	4	14	theme	contact	792:798	arg1	time					800:803	contact time	792:803	contact time	792:803	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	3	15	theme	as-prepared	504:514	arg1	materials					516:524	as-prepared materials	504:524	as-prepared materials	504:524	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	16	dep	Fourier	548:554	arg1	transform					556:564	transform	556:564	transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM)	556:754	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	0	17	dep	Cr	106:107	arg1	VI					109:110	VI	109:110	VI	109:110	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	2	18	theme	cross-linking	417:429	arg1	agent					431:435	cross-linking agent	417:435	cross-linking agent	417:435	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	3	19	theme	scanning	721:728	arg1	SEM					751:753	SEM	751:753	SEM	751:753	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	19	theme	scanning	721:728	arg1	microscopy					739:748	scanning electron microscopy	721:748	scanning electron microscopy (SEM)	721:754	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	2	20	theme	sponge-like	285:295	arg1	CS					314:315	CS	314:315	CS	314:315	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	2	20	theme	sponge-like	285:295	arg1	kaolin/chitosan					297:311	the sponge-like kaolin/chitosan	281:311	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	0	21	theme	kaolin/chitosan/reduced	29:51	arg1	composite					68:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	3	22	theme	electron	730:737	arg1	SEM					751:753	SEM	751:753	SEM	751:753	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	22	theme	electron	730:737	arg1	microscopy					739:748	scanning electron microscopy	721:748	scanning electron microscopy (SEM)	721:754	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	5	23	theme	good	958:961	arg1	capacity					974:981	good absorption capacity	958:981	good absorption capacity for Cr(VI) and alizarin yellow R (AYR)	958:1020	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	2	24	theme	simple	388:393	arg1	self-assembly					395:407	simple self-assembly	388:407	simple self-assembly without cross-linking agent	388:435	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	0	25	theme	sponge-like	17:27	arg1	composite					68:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	0	26	theme	AYR	117:119	arg1	adsorption					92:101	adsorption	92:101	adsorption of Cr(VI) and AYR	92:119	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	1	27	theme	3D	186:187	arg1	hydrogels					189:197	3D hydrogels	186:197	3D hydrogels	186:197	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	0	28	theme	oxide	62:66	arg1	composite					68:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	6	29	theme	composite	1032:1040	arg1	hydrogel					1042:1049	composite hydrogel	1032:1049	composite hydrogel	1032:1049	Besides, composite hydrogel also exhibits excellent flexibility and good repeatability, which confirms its great potential as an adsorbent to remove pollutants in the water environment.
31385250	5	30	theme	alizarin	998:1005	arg1	AYR					1017:1019	AYR	1017:1019	AYR	1017:1019	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	5	30	theme	alizarin	998:1005	arg1	R					1014:1014	alizarin yellow R	998:1014	alizarin yellow R (AYR)	998:1020	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	4	31	theme	material	776:783	arg1	effects					761:767	The effects	757:767	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance	757:883	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	time					800:803	contact time	792:803	contact time	792:803	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	strength					829:836	ionic strength	823:836	ionic strength	823:836	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	temperature					806:816	temperature	806:816	temperature	806:816	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	ratio					785:789	ratio	785:789	ratio	785:789	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	pH					819:820	pH	819:820	pH	819:820	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	4	32	dep	material	776:783	arg1	times					853:857	recycling times	843:857	recycling times	843:857	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	0	33	theme	graphene	53:60	arg1	composite					68:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	sponge-like kaolin/chitosan/reduced graphene oxide composite	17:76	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	4	34	theme	adsorption	862:871	arg1	performance					873:883	adsorption performance	862:883	adsorption performance	862:883	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	5	35	theme	yellow	1007:1012	arg1	AYR					1017:1019	AYR	1017:1019	AYR	1017:1019	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	5	35	theme	yellow	1007:1012	arg1	R					1014:1014	alizarin yellow R	998:1014	alizarin yellow R (AYR)	998:1020	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	4	36	theme	raw	772:774	arg1	material					776:783	raw material ratio, contact time, temperature, pH, ionic strength, and recycling times	772:857	raw material ratio, contact time, temperature, pH, ionic strength, and recycling times	772:857	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	3	37	theme	infrared	566:573	arg1	FT-IR					589:593	FT-IR	589:593	FT-IR	589:593	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	37	theme	infrared	566:573	arg1	spectroscopy					575:586	infrared spectroscopy	566:586	infrared spectroscopy (FT-IR)	566:594	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	2	38	theme	/reduced	317:324	arg1	composite					347:355	/reduced graphene oxide (rGO) composite	317:355	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	0	39	theme	composite	68:76	arg1	Self-assembly					0:12	Self-assembly	0:12	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite	0:76	Self-assembly of sponge-like kaolin/chitosan/reduced graphene oxide composite hydrogels for adsorption of Cr(VI) and AYR.
31385250	3	40	theme	potential	642:650	arg1	analyzer					652:659	zeta potential analyzer	637:659	zeta potential analyzer	637:659	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	6	41	theme	water	1190:1194	arg1	environment					1196:1206	the water environment	1186:1206	the water environment	1186:1206	Besides, composite hydrogel also exhibits excellent flexibility and good repeatability, which confirms its great potential as an adsorbent to remove pollutants in the water environment.
31385250	4	42	theme	recycling	843:851	arg1	times					853:857	recycling times	843:857	recycling times	843:857	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	6	43	theme	great	1130:1134	arg1	potential					1136:1144	its great potential	1126:1144	its great potential as an adsorbent to remove pollutants in the water environment	1126:1206	Besides, composite hydrogel also exhibits excellent flexibility and good repeatability, which confirms its great potential as an adsorbent to remove pollutants in the water environment.
31385250	3	44	theme	X-ray	597:601	arg1	XPS					631:633	XPS	631:633	XPS	631:633	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	44	theme	X-ray	597:601	arg1	spectroscopy					617:628	X-ray photoelectron spectroscopy	597:628	X-ray photoelectron spectroscopy (XPS)	597:634	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	5	45	theme	absorption	963:972	arg1	capacity					974:981	good absorption capacity	958:981	good absorption capacity for Cr(VI) and alizarin yellow R (AYR)	958:1020	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	2	46	dep	kaolin/chitosan	297:311	arg1	composite					347:355	/reduced graphene oxide (rGO) composite	317:355	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	1	47	theme	excellent	204:212	arg1	properties					225:234	excellent mechanical properties	204:234	excellent mechanical properties	204:234	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	3	48	theme	photoelectron	603:615	arg1	XPS					631:633	XPS	631:633	XPS	631:633	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	48	theme	photoelectron	603:615	arg1	spectroscopy					617:628	X-ray photoelectron spectroscopy	597:628	X-ray photoelectron spectroscopy (XPS)	597:634	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	1	49	theme	mechanical	214:223	arg1	properties					225:234	excellent mechanical properties	204:234	excellent mechanical properties	204:234	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	3	50	theme	zeta	637:640	arg1	analyzer					652:659	zeta potential analyzer	637:659	zeta potential analyzer	637:659	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	4	51	from	effects	761:767	arg1	performance					873:883	adsorption performance	862:883	adsorption performance	862:883	The effects of raw material ratio, contact time, temperature, pH, ionic strength, and recycling times on adsorption performance were investigated in detail.
31385250	2	52	theme	rGO	342:344	arg1	composite					347:355	/reduced graphene oxide (rGO) composite	317:355	the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite	281:355	In this work, the sponge-like kaolin/chitosan (CS)/reduced graphene oxide (rGO) composite was prepared for adsorption by simple self-assembly without cross-linking agent.
31385250	5	53	dep	Cr	987:988	arg1	VI					990:991	VI	990:991	VI	990:991	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	3	54	theme	surface	685:691	arg1	BET					711:713	BET	711:713	BET	711:713	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	3	54	theme	surface	685:691	arg1	measurement					698:708	Brunauer-Emmett-Teller surface area measurement	662:708	Brunauer-Emmett-Teller surface area measurement (BET)	662:714	The morphology, composition, surface properties, and pore size of as-prepared materials were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, Brunauer-Emmett-Teller surface area measurement (BET), and scanning electron microscopy (SEM).
31385250	1	55	theme	graphene	138:145	arg1	oxide					147:151	graphene oxide	138:151	graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties	138:234	The assembly of graphene oxide with biomass or polymers to form 3D hydrogels with excellent mechanical properties has become a research hotspot.
31385250	5	56	contain	has	954:956	arg1	composite					944:952	the composite	940:952	the composite	940:952	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
31385250	5	56	contain	has	954:956	arg2	capacity					974:981	good absorption capacity	958:981	good absorption capacity for Cr(VI) and alizarin yellow R (AYR)	958:1020	The results indicate that the composite has good absorption capacity for Cr(VI) and alizarin yellow R (AYR).
30652296	1	0	theme	analytical	145:154	arg1	tools					156:160	the analytical tools	141:160	the analytical tools	141:160	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	2	1	theme	starch	298:303	arg1	steps					271:275	several steps	263:275	several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis	263:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	1	theme	starch	298:303	arg1	determination					305:317	starch determination	298:317	starch determination	298:317	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	4	2	theme	specific	676:683	arg1	consideration					685:697	specific consideration	676:697	specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition	676:804	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	5	3	dep	extraction	840:849	arg1	the					836:838	the	836:838	the	836:838	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	1	4	theme	NREL	61:64	arg1	suite					66:70	The publicly available NREL suite	38:70	The publicly available NREL suite of laboratory analytical procedures (LAPs)	38:113	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	5	5	from	cellulose	914:922	arg1	glucan					902:907	glucan	902:907	glucan	902:907	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	5	from	cellulose	914:922	arg1	starch					891:896	starch	891:896	starch	891:896	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	4	6	theme	types	720:724	arg1	consideration					685:697	specific consideration	676:697	specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition	676:804	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	3	7	theme	compounds	444:452	arg1	classes					433:439	classes	433:439	classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin	433:568	These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin.
30652296	3	7	theme	compounds	444:452	arg1	information					395:405	information	395:405	information on specific compounds	395:427	These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin.
30652296	5	8	with	hybrids	931:937	arg1	amounts					956:962	significant amounts	944:962	significant amounts of nonstructural carbohydrates	944:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	8	with	hybrids	931:937	arg1	carbohydrates					981:993	nonstructural carbohydrates	967:993	nonstructural carbohydrates	967:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	1	9	with	researchers	124:134	arg1	tools					156:160	the analytical tools	141:160	the analytical tools	141:160	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	2	10	theme	sample	278:283	arg1	steps					271:275	several steps	263:275	several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis	263:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	10	theme	sample	278:283	arg1	preparation					285:295	sample preparation	278:295	sample preparation	278:295	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	5	11	from	glucan	902:907	arg1	differentiation					859:873	the differentiation	855:873	the differentiation of glucose	855:884	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	11	from	glucan	902:907	arg1	hybrids					931:937	the hybrids	927:937	the hybrids with significant amounts of nonstructural carbohydrates	927:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	11	from	glucan	902:907	arg1	extraction					840:849	extraction	840:849	extraction	840:849	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	3	12	from	information	395:405	arg1	compounds					419:427	specific compounds	410:427	specific compounds	410:427	These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin.
30652296	1	13	theme	laboratory	75:84	arg1	LAPs					109:112	LAPs	109:112	LAPs	109:112	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	1	13	theme	laboratory	75:84	arg1	procedures					97:106	laboratory analytical procedures	75:106	laboratory analytical procedures (LAPs)	75:113	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	2	14	theme	moisture	320:327	arg1	steps					271:275	several steps	263:275	several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis	263:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	14	theme	moisture	320:327	arg1	analysis					329:336	moisture analysis	320:336	moisture analysis	320:336	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	4	15	theme	main	707:710	arg1	sweet					742:746	sweet	742:746	sweet	742:746	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	15	theme	main	707:710	arg1	biomass					753:759	biomass	753:759	biomass	753:759	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	15	theme	main	707:710	arg1	grain					727:731	grain	727:731	grain	727:731	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	15	theme	main	707:710	arg1	types					720:724	four main sorghum types	702:724	four main sorghum types	702:724	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	2	16	dep	steps	271:275	arg1	analysis					329:336	moisture analysis	320:336	moisture analysis	320:336	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	hydrolysis					363:372	hydrolysis	363:372	hydrolysis	363:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	preparation					285:295	sample preparation	278:295	sample preparation	278:295	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	ashing					339:344	ashing	339:344	ashing	339:344	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	extraction					347:356	extraction	347:356	extraction	347:356	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	steps					271:275	several steps	263:275	several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis	263:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	16	dep	steps	271:275	arg1	determination					305:317	starch determination	298:317	starch determination	298:317	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	0	17	theme	Fuel	21:24	arg1	Production					26:35	Fuel Production	21:35	Fuel Production	21:35	Assaying Sorghum for Fuel Production.
30652296	1	18	theme	procedures	97:106	arg1	suite					66:70	The publicly available NREL suite	38:70	The publicly available NREL suite of laboratory analytical procedures (LAPs)	38:113	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	5	19	theme	glucose	878:884	arg1	differentiation					859:873	the differentiation	855:873	the differentiation of glucose	855:884	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	19	theme	glucose	878:884	arg1	extraction					840:849	extraction	840:849	extraction	840:849	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	1	20	theme	analytical	86:95	arg1	LAPs					109:112	LAPs	109:112	LAPs	109:112	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	1	20	theme	analytical	86:95	arg1	procedures					97:106	laboratory analytical procedures	75:106	laboratory analytical procedures (LAPs)	75:113	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	1	21	theme	woody	194:198	arg1	feedstocks					215:224	woody and herbaceous feedstocks	194:224	woody and herbaceous feedstocks	194:224	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	4	22	dep	types	720:724	arg1	sweet					742:746	sweet	742:746	sweet	742:746	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	22	dep	types	720:724	arg1	biomass					753:759	biomass	753:759	biomass	753:759	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	22	dep	types	720:724	arg1	forage					734:739	forage	734:739	forage	734:739	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	22	dep	types	720:724	arg1	grain					727:731	grain	727:731	grain	727:731	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	22	dep	types	720:724	arg1	types					720:724	four main sorghum types	702:724	four main sorghum types	702:724	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	23	theme	procedures	606:615	arg1	use					593:595	the use	589:595	the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition	589:804	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	2	24	theme	Feedstock	227:235	arg1	characterization					237:252	Feedstock characterization	227:252	Feedstock characterization	227:252	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	1	25	theme	herbaceous	204:213	arg1	feedstocks					215:224	woody and herbaceous feedstocks	194:224	woody and herbaceous feedstocks	194:224	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	3	26	theme	specific	410:417	arg1	compounds					419:427	specific compounds	410:427	specific compounds	410:427	These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin.
30652296	5	27	theme	significant	944:954	arg1	amounts					956:962	significant amounts	944:962	significant amounts of nonstructural carbohydrates	944:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	27	theme	significant	944:954	arg1	carbohydrates					981:993	nonstructural carbohydrates	967:993	nonstructural carbohydrates	967:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	4	28	theme	L.	659:660	arg1	bicolor					650:656	sorghum [Sorghum bicolor	633:656	sorghum [Sorghum bicolor (L.) Moench]	633:669	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	28	theme	L.	659:660	arg1	Moench					663:668	L.) Moench	659:668	L.) Moench	659:668	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	1	29	theme	feedstocks	215:224	arg1	composition					179:189	the composition	175:189	the composition of woody and herbaceous feedstocks	175:224	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	4	30	theme	sorghum	712:718	arg1	sweet					742:746	sweet	742:746	sweet	742:746	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	30	theme	sorghum	712:718	arg1	biomass					753:759	biomass	753:759	biomass	753:759	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	30	theme	sorghum	712:718	arg1	grain					727:731	grain	727:731	grain	727:731	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	30	theme	sorghum	712:718	arg1	types					720:724	four main sorghum types	702:724	four main sorghum types	702:724	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	5	31	theme	nonstructural	967:979	arg1	carbohydrates					981:993	nonstructural carbohydrates	967:993	nonstructural carbohydrates	967:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	1	32	theme	available	51:59	arg1	suite					66:70	The publicly available NREL suite	38:70	The publicly available NREL suite of laboratory analytical procedures (LAPs)	38:113	The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks.
30652296	3	33	from	classes	433:439	arg1	compounds					419:427	specific compounds	410:427	specific compounds	410:427	These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin.
30652296	5	34	theme	Special	807:813	arg1	attention					815:823	Special attention	807:823	Special attention	807:823	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	35	theme	carbohydrates	981:993	arg1	amounts					956:962	significant amounts	944:962	significant amounts of nonstructural carbohydrates	944:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	35	theme	carbohydrates	981:993	arg1	carbohydrates					981:993	nonstructural carbohydrates	967:993	nonstructural carbohydrates	967:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	2	36	theme	several	263:269	arg1	analysis					329:336	moisture analysis	320:336	moisture analysis	320:336	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	hydrolysis					363:372	hydrolysis	363:372	hydrolysis	363:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	preparation					285:295	sample preparation	278:295	sample preparation	278:295	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	ashing					339:344	ashing	339:344	ashing	339:344	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	extraction					347:356	extraction	347:356	extraction	347:356	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	steps					271:275	several steps	263:275	several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis	263:372	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	2	36	theme	several	263:269	arg1	determination					305:317	starch determination	298:317	starch determination	298:317	Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis.
30652296	4	37	theme	[Sorghum	641:648	arg1	bicolor					650:656	sorghum [Sorghum bicolor	633:656	sorghum [Sorghum bicolor (L.) Moench]	633:669	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	37	theme	[Sorghum	641:648	arg1	Moench					663:668	L.) Moench	659:668	L.) Moench	659:668	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	38	theme	sorghum	633:639	arg1	bicolor					650:656	sorghum [Sorghum bicolor	633:656	sorghum [Sorghum bicolor (L.) Moench]	633:669	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	4	38	theme	sorghum	633:639	arg1	Moench					663:668	L.) Moench	659:668	L.) Moench	659:668	Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition.
30652296	5	39	from	starch	891:896	arg1	differentiation					859:873	the differentiation	855:873	the differentiation of glucose	855:884	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	39	from	starch	891:896	arg1	hybrids					931:937	the hybrids	927:937	the hybrids with significant amounts of nonstructural carbohydrates	927:993	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
30652296	5	39	from	starch	891:896	arg1	extraction					840:849	extraction	840:849	extraction	840:849	Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
29872975	6	0	theme	Good	1292:1295	arg1	Practices					1311:1319	Current Good Manufacturing Practices	1284:1319	Current Good Manufacturing Practices for dietary supplements	1284:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	2	1	dep	specific	481:488	arg1	needs					490:494	needs	490:494	needs	490:494	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	6	2	theme	pharmaceutical-grade	1091:1110	arg1	polymers					1112:1119	pharmaceutical-grade polymers	1091:1119	pharmaceutical-grade polymers	1091:1119	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	0	3	from	Development	11:21	arg1	Form					78:81	the Form	74:81	the Form of a Multicompartment HPC Capsule	74:115	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	6	4	theme	Current	1284:1290	arg1	Practices					1311:1319	Current Good Manufacturing Practices	1284:1319	Current Good Manufacturing Practices for dietary supplements	1284:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	5	5	theme	work	893:896	arg1	aim					881:883	the aim	877:883	the aim of this work	877:896	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	7	6	with	compliance	1488:1497	arg1	specifications					1587:1600	internal specifications	1578:1600	internal specifications	1578:1600	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	0	7	theme	Multicompartment	88:103	arg1	Capsule					109:115	a Multicompartment HPC Capsule	86:115	a Multicompartment HPC Capsule	86:115	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	3	8	theme	dietary	691:697	arg1	supplements					699:709	dietary supplements	691:709	dietary supplements	691:709	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	5	9	theme	oral	1016:1019	arg1	delivery					1031:1038	oral pulsatile delivery	1016:1038	oral pulsatile delivery	1016:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	8	10	theme	operating	1625:1633	arg1	charts					1635:1640	operating charts	1625:1640	operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality	1625:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	5	11	theme	processes	952:960	arg1	development					933:943	early-stage industrial development	910:943	early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery	910:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	5	12	theme	nutraceutical	989:1001	arg1	capsules					1003:1010	nutraceutical capsules	989:1010	nutraceutical capsules for oral pulsatile delivery	989:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	6	13	theme	3D	1241:1242	arg1	printing					1244:1251	3D printing	1241:1251	3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements	1241:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	5	14	theme	industrial	922:931	arg1	development					933:943	early-stage industrial development	910:943	early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery	910:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	1	15	with	combination	242:252	arg1	treatments					264:273	drug treatments	259:273	drug treatments	259:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	8	16	theme	critical	1655:1662	arg1	parameters					1686:1695	parameters	1686:1695	parameters	1686:1695	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	16	theme	critical	1655:1662	arg1	variables					1672:1680	critical process variables	1655:1680	critical process variables	1655:1680	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	16	theme	critical	1655:1662	arg1	indices					1717:1723	indices	1717:1723	indices of both intermediate and final product quality	1717:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	4	17	theme	separate	730:737	arg1	compartments					745:756	separate inner compartments	730:756	separate inner compartments	730:756	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	1	18	theme	drug	259:262	arg1	treatments					264:273	drug treatments	259:273	drug treatments	259:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	0	19	theme	Capsule	109:115	arg1	Form					78:81	the Form	74:81	the Form of a Multicompartment HPC Capsule	74:115	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	4	20	theme	release	844:850	arg1	kinetics					852:859	release kinetics	844:859	release kinetics	844:859	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	7	21	theme	by-products	1560:1570	arg1	compliance					1488:1497	compliance	1488:1497	compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications	1488:1600	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	8	22	theme	final	1750:1754	arg1	quality					1764:1770	both intermediate and final product quality	1728:1770	both intermediate and final product quality	1728:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	0	23	theme	HPC	105:107	arg1	Capsule					109:115	a Multicompartment HPC Capsule	86:115	a Multicompartment HPC Capsule	86:115	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	1	24	theme	release	354:360	arg1	performance					362:372	release performance	354:372	release performance	354:372	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	6	25	theme	capsule	1256:1262	arg1	shells					1264:1269	capsule shells	1256:1269	capsule shells according to Current Good Manufacturing Practices for dietary supplements	1256:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	6	26	theme	filaments	1072:1080	arg1	extrusion					1059:1067	extrusion	1059:1067	extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing	1059:1148	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	2	27	theme	products	455:462	arg1	possibility					428:438	the possibility	424:438	the possibility of having such products tailored to meet specific needs	424:494	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	7	28	theme	items	1460:1464	arg1	stability					1383:1391	the stability	1379:1391	the stability of the starting material after hot processing and of the resulting items	1379:1464	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	1	29	from	advances	135:142	arg1	nutrigenomics					147:159	nutrigenomics	147:159	nutrigenomics	147:159	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	29	from	advances	135:142	arg1	nutrigenetics					165:177	nutrigenetics	165:177	nutrigenetics	165:177	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	3	30	theme	market	555:560	arg1	trends					562:567	these emerging market trends	540:567	these emerging market trends	540:567	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	8	31	theme	intermediate	1733:1744	arg1	quality					1764:1770	both intermediate and final product quality	1728:1770	both intermediate and final product quality	1728:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	7	32	theme	microbiological	1516:1530	arg1	contaminants					1532:1543	elemental and microbiological contaminants	1502:1543	elemental and microbiological contaminants	1502:1543	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	0	33	theme	Industrial	0:9	arg1	Development					11:21	Industrial Development	0:21	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.	0:116	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	6	34	theme	shells	1264:1269	arg1	printing					1244:1251	3D printing	1241:1251	3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements	1241:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	4	35	theme	kinetics	852:859	arg1	combinations					796:807	customized combinations	785:807	customized combinations of substances, relevant doses, and release kinetics	785:859	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	4	36	theme	inner	739:743	arg1	compartments					745:756	separate inner compartments	730:756	separate inner compartments	730:756	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	8	37	theme	product	1756:1762	arg1	quality					1764:1770	both intermediate and final product quality	1728:1770	both intermediate and final product quality	1728:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	3	38	theme	drugs	655:659	arg1	administration					637:650	administration	637:650	administration	637:650	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	3	38	theme	drugs	655:659	arg1	conveyance					622:631	conveyance	622:631	conveyance	622:631	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	5	39	theme	capsules	1003:1010	arg1	fabrication					974:984	fabrication	974:984	fabrication of nutraceutical capsules for oral pulsatile delivery	974:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	4	40	theme	doses	833:837	arg1	combinations					796:807	customized combinations	785:807	customized combinations of substances, relevant doses, and release kinetics	785:859	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	7	41	theme	starting	1400:1407	arg1	material					1409:1416	the starting material	1396:1416	the starting material after hot processing	1396:1437	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	4	42	theme	relevant	824:831	arg1	doses					833:837	relevant doses	824:837	relevant doses	824:837	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	7	43	theme	hot	1424:1426	arg1	processing					1428:1437	hot processing	1424:1437	hot processing	1424:1437	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	1	44	theme	considerable	276:287	arg1	attention					289:297	considerable attention	276:297	considerable attention	276:297	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	3	45	theme	emerging	546:553	arg1	trends					562:567	these emerging market trends	540:567	these emerging market trends	540:567	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	0	46	theme	Nutraceutical	39:51	arg1	Platform					62:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	1	47	theme	dietary	377:383	arg1	supplements					385:395	dietary supplements	377:395	dietary supplements	377:395	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	3	48	theme	capsular	581:588	arg1	devices					590:596	3D-printed capsular devices	570:596	3D-printed capsular devices originally intended for conveyance and administration of drugs	570:659	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	8	49	theme	quality	1764:1770	arg1	parameters					1686:1695	parameters	1686:1695	parameters	1686:1695	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	49	theme	quality	1764:1770	arg1	variables					1672:1680	critical process variables	1655:1680	critical process variables	1655:1680	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	49	theme	quality	1764:1770	arg1	indices					1717:1723	indices	1717:1723	indices of both intermediate and final product quality	1717:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	6	50	theme	pilot	1043:1047	arg1	plant					1049:1053	A pilot plant	1041:1053	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing	1041:1148	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	0	51	theme	3D-Printed	28:37	arg1	Platform					62:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	1	52	from	combination	242:252	arg1	view					194:197	view	194:197	view of the increasing use of nutraceuticals in combination with drug treatments	194:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	53	theme	supplements	385:395	arg1	bioefficacy					337:347	bioefficacy	337:347	bioefficacy	337:347	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	53	theme	supplements	385:395	arg1	composition					324:334	the composition	320:334	the composition	320:334	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	53	theme	supplements	385:395	arg1	performance					362:372	release performance	354:372	release performance	354:372	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	5	54	theme	pulsatile	1021:1029	arg1	delivery					1031:1038	oral pulsatile delivery	1016:1038	oral pulsatile delivery	1016:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	6	55	theme	Manufacturing	1297:1309	arg1	Practices					1311:1319	Current Good Manufacturing Practices	1284:1319	Current Good Manufacturing Practices for dietary supplements	1284:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	0	56	theme	Platform	62:69	arg1	Development					11:21	Industrial Development	0:21	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.	0:116	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	6	57	theme	dietary	1325:1331	arg1	supplements					1333:1343	dietary supplements	1325:1343	dietary supplements	1325:1343	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	6	58	theme	modeling	1229:1236	arg1	feasibility					1197:1207	feasibility	1197:1207	feasibility of fused deposition modeling	1197:1236	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	7	59	theme	resulting	1450:1458	arg1	items					1460:1464	the resulting items	1446:1464	the resulting items	1446:1464	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	0	60	theme	Delivery	53:60	arg1	Platform					62:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	a 3D-Printed Nutraceutical Delivery Platform	26:69	Industrial Development of a 3D-Printed Nutraceutical Delivery Platform in the Form of a Multicompartment HPC Capsule.
29872975	8	61	theme	process	1664:1670	arg1	parameters					1686:1695	parameters	1686:1695	parameters	1686:1695	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	61	theme	process	1664:1670	arg1	variables					1672:1680	critical process variables	1655:1680	critical process variables	1655:1680	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	8	61	theme	process	1664:1670	arg1	indices					1717:1723	indices	1717:1723	indices of both intermediate and final product quality	1717:1770	Finally, operating charts highlighting critical process variables and parameters that would serve as indices of both intermediate and final product quality were developed.
29872975	2	62	theme	having	443:448	arg1	products					455:462	having such products	443:462	having such products	443:462	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	3	63	theme	3D-printed	570:579	arg1	devices					590:596	3D-printed capsular devices	570:596	3D-printed capsular devices originally intended for conveyance and administration of drugs	570:659	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	6	64	theme	3D	1138:1139	arg1	printing					1141:1148	3D printing	1138:1148	3D printing	1138:1148	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	6	65	theme	deposition	1218:1227	arg1	modeling					1229:1236	fused deposition modeling	1212:1236	fused deposition modeling	1212:1236	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	7	66	theme	contaminants	1532:1543	arg1	compliance					1488:1497	compliance	1488:1497	compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications	1488:1600	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	6	67	theme	fused	1212:1216	arg1	modeling					1229:1236	fused deposition modeling	1212:1236	fused deposition modeling	1212:1236	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	1	68	from	view	194:197	arg1	combination					242:252	combination	242:252	combination with drug treatments	242:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	69	theme	recent	128:133	arg1	advances					135:142	recent advances	128:142	recent advances in nutrigenomics and nutrigenetics	128:177	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	70	theme	increasing	206:215	arg1	use					217:219	the increasing use	202:219	the increasing use of nutraceuticals in combination with drug treatments	202:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	1	71	from	use	217:219	arg1	combination					242:252	combination	242:252	combination with drug treatments	242:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	7	72	theme	elemental	1502:1510	arg1	contaminants					1532:1543	elemental and microbiological contaminants	1502:1543	elemental and microbiological contaminants	1502:1543	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	4	73	theme	customized	785:794	arg1	combinations					796:807	customized combinations	785:807	customized combinations of substances, relevant doses, and release kinetics	785:859	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	2	74	dep	growing	504:510	arg1	fast					499:502	fast	499:502	fast	499:502	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	5	75	theme	early-stage	910:920	arg1	development					933:943	early-stage industrial development	910:943	early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery	910:1038	In particular, the aim of this work was to face early-stage industrial development of the processes involved in fabrication of nutraceutical capsules for oral pulsatile delivery.
29872975	1	76	theme	use	217:219	arg1	view					194:197	view	194:197	view of the increasing use of nutraceuticals in combination with drug treatments	194:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
29872975	6	77	dep	filaments	1072:1080	arg1	intended					1125:1132	intended	1125:1132	intended for 3D printing	1125:1148	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	6	77	dep	filaments	1072:1080	arg1	based					1082:1086	based	1082:1086	based on pharmaceutical-grade polymers	1082:1119	A pilot plant for extrusion of filaments based on pharmaceutical-grade polymers and intended for 3D printing was set up, and studies aimed at demonstrating feasibility of fused deposition modeling in 3D printing of capsule shells according to Current Good Manufacturing Practices for dietary supplements were undertaken.
29872975	4	78	theme	substances	812:821	arg1	combinations					796:807	customized combinations	785:807	customized combinations of substances, relevant doses, and release kinetics	785:859	Being composed of separate inner compartments, such a device could yield customized combinations of substances, relevant doses, and release kinetics.
29872975	7	79	theme	internal	1578:1585	arg1	specifications					1587:1600	internal specifications	1578:1600	internal specifications	1578:1600	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	2	80	theme	such	450:453	arg1	products					455:462	having such products	443:462	having such products	443:462	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	3	81	theme	supplements	699:709	arg1	delivery					679:686	delivery	679:686	delivery of dietary supplements	679:709	To fulfill these emerging market trends, 3D-printed capsular devices originally intended for conveyance and administration of drugs were proposed for delivery of dietary supplements.
29872975	2	82	from	interest	412:419	arg1	possibility					428:438	the possibility	424:438	the possibility of having such products tailored to meet specific needs	424:494	Moreover, the interest in the possibility of having such products tailored to meet specific needs is fast growing among costumers.
29872975	7	83	theme	material	1409:1416	arg1	stability					1383:1391	the stability	1379:1391	the stability of the starting material after hot processing and of the resulting items	1379:1464	In this respect, the stability of the starting material after hot processing and of the resulting items was investigated, and compliance of elemental and microbiological contaminants, as well as of by-products, with internal specifications was assessed.
29872975	1	84	theme	nutraceuticals	224:237	arg1	use					217:219	the increasing use	202:219	the increasing use of nutraceuticals in combination with drug treatments	202:273	Following recent advances in nutrigenomics and nutrigenetics, as well as in view of the increasing use of nutraceuticals in combination with drug treatments, considerable attention is being directed to the composition, bioefficacy, and release performance of dietary supplements.
31816372	2	0	theme	casting	481:487	arg1	technique					489:497	the casting technique	477:497	the casting technique	477:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	1	1	theme	food	204:207	arg1	industry					219:226	food packaging industry	204:226	food packaging industry instead of petroleum-based packaging materials	204:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	4	2	from	ratio	746:750	arg1	matrix					759:764	the matrix	755:764	the matrix	755:764	All physicochemical properties and antioxidant activities were significantly affected by the chitosan ascorbate/methylcellulose ratio in the matrix.
31816372	6	3	theme	composite	1043:1051	arg1	films					1053:1057	the composite films	1039:1057	the composite films containing a greater proportion of chitosan ascorbate	1039:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	5	4	theme	break	819:823	arg1	values					825:830	break values	819:830	break values	819:830	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	compactness					884:894	compactness	884:894	compactness	884:894	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	elongation					805:814	elongation	805:814	elongation	805:814	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	reduction					921:929	a reduction	919:929	a reduction in water vapor permeability	919:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	temperatures					855:866	maximum decomposition temperatures	833:866	maximum decomposition temperatures	833:866	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	increases					771:779	The increases	767:779	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability	767:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	content					906:912	moisture content	897:912	moisture content	897:912	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	index					877:881	whitish index	869:881	whitish index	869:881	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	5	from	values	825:830	arg1	strength					792:799	tensile strength	784:799	tensile strength	784:799	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	6	theme	water	934:938	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	1	7	theme	packaging	209:217	arg1	industry					219:226	food packaging industry	204:226	food packaging industry instead of petroleum-based packaging materials	204:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	7	8	theme	composite	1281:1289	arg1	films					1291:1295	The chitosan ascorbate/methylcellulose composite films	1242:1295	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties	1242:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	7	9	theme	food	1443:1446	arg1	packaging					1448:1456	food packaging	1443:1456	food packaging	1443:1456	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	2	10	theme	chitosan	337:344	arg1	ascorbate					346:354	chitosan ascorbate	337:354	chitosan ascorbate	337:354	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	5	11	theme	methylcellulose	994:1008	arg1	proportion					980:989	the proportion	976:989	the proportion of methylcellulose	976:1008	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	7	12	theme	ascorbate/methylcellulose	1255:1279	arg1	films					1291:1295	The chitosan ascorbate/methylcellulose composite films	1242:1295	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties	1242:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	6	13	contain	containing	1059:1068	arg2	proportion					1080:1089	a greater proportion	1070:1089	a greater proportion of chitosan ascorbate	1070:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	6	13	contain	containing	1059:1068	arg1	films					1053:1057	the composite films	1039:1057	the composite films containing a greater proportion of chitosan ascorbate	1039:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	5	14	from	reduction	921:929	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	3	15	theme	DPPH	548:551	arg1	ability					572:578	the DPPH radical scavenging ability	544:578	the DPPH radical scavenging ability	544:578	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power were investigated.
31816372	5	16	theme	tensile	784:790	arg1	strength					792:799	tensile strength	784:799	tensile strength	784:799	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	17	from	index	877:881	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	6	18	theme	chitosan	1094:1101	arg1	ascorbate					1103:1111	chitosan ascorbate	1094:1111	chitosan ascorbate	1094:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	6	19	theme	barrier	1134:1140	arg1	properties					1142:1151	the better barrier properties	1123:1151	the better barrier properties	1123:1151	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	5	20	theme	decomposition	841:853	arg1	temperatures					855:866	maximum decomposition temperatures	833:866	maximum decomposition temperatures	833:866	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	21	theme	vapor	940:944	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	7	22	theme	edible	1419:1424	arg1	biomaterials					1426:1437	biodegradable and edible biomaterials	1401:1437	biodegradable and edible biomaterials	1401:1437	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	1	23	theme	Polysaccharide-based	110:129	arg1	films					145:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	1	23	theme	Polysaccharide-based	110:129	arg1	candidates					189:198	the promising candidates	175:198	the promising candidates for food packaging industry instead of petroleum-based packaging materials	175:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	6	24	theme	better	1127:1132	arg1	properties					1142:1151	the better barrier properties	1123:1151	the better barrier properties	1123:1151	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	7	25	theme	interesting	1302:1312	arg1	properties					1330:1339	interesting physicochemical properties	1302:1339	interesting physicochemical properties	1302:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	1	26	theme	biodegradable	131:143	arg1	films					145:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	1	26	theme	biodegradable	131:143	arg1	candidates					189:198	the promising candidates	175:198	the promising candidates for food packaging industry instead of petroleum-based packaging materials	175:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	6	27	theme	ascorbate	1103:1111	arg1	proportion					1080:1089	a greater proportion	1070:1089	a greater proportion of chitosan ascorbate	1070:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	1	28	theme	petroleum-based	239:253	arg1	materials					265:273	petroleum-based packaging materials	239:273	petroleum-based packaging materials	239:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	5	29	theme	whitish	869:875	arg1	index					877:881	whitish index	869:881	whitish index	869:881	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	7	30	theme	antioxidant	1352:1362	arg1	action					1364:1369	strong antioxidant action	1345:1369	strong antioxidant action	1345:1369	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	3	31	theme	radical	553:559	arg1	ability					572:578	the DPPH radical scavenging ability	544:578	the DPPH radical scavenging ability	544:578	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power were investigated.
31816372	1	32	theme	packaging	255:263	arg1	materials					265:273	petroleum-based packaging materials	239:273	petroleum-based packaging materials	239:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	0	33	theme	physicochemical	16:30	arg1	properties					32:41	physicochemical properties	16:41	physicochemical properties	16:41	Preparation and physicochemical properties of antioxidant chitosan ascorbate/methylcellulose composite films.
31816372	6	34	theme	UV-vis	1161:1166	arg1	light					1168:1172	UV-vis light	1161:1172	UV-vis light	1161:1172	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	3	35	theme	scavenging	561:570	arg1	ability					572:578	the DPPH radical scavenging ability	544:578	the DPPH radical scavenging ability	544:578	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power were investigated.
31816372	1	36	theme	materials	265:273	arg1	industry					219:226	food packaging industry	204:226	food packaging industry instead of petroleum-based packaging materials	204:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	0	37	theme	antioxidant	46:56	arg1	chitosan					58:65	antioxidant chitosan	46:65	antioxidant chitosan	46:65	Preparation and physicochemical properties of antioxidant chitosan ascorbate/methylcellulose composite films.
31816372	6	38	theme	stronger	1182:1189	arg1	effect					1215:1220	the stronger DPPH radical scavenging effect	1178:1220	the stronger DPPH radical scavenging effect	1178:1220	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	7	39	theme	chitosan	1246:1253	arg1	films					1291:1295	The chitosan ascorbate/methylcellulose composite films	1242:1295	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties	1242:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	5	40	from	strength	792:799	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	7	41	with	action	1364:1369	arg1	properties					1330:1339	interesting physicochemical properties	1302:1339	interesting physicochemical properties	1302:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	7	42	theme	biodegradable	1401:1413	arg1	biomaterials					1426:1437	biodegradable and edible biomaterials	1401:1437	biodegradable and edible biomaterials	1401:1437	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	2	43	theme	biopolymers	459:469	arg1	ratios					405:410	different ratios	395:410	different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique	395:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	5	44	from	increases	771:779	arg1	compactness					884:894	compactness	884:894	compactness	884:894	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	elongation					805:814	elongation	805:814	elongation	805:814	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	reduction					921:929	a reduction	919:929	a reduction in water vapor permeability	919:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	temperatures					855:866	maximum decomposition temperatures	833:866	maximum decomposition temperatures	833:866	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	content					906:912	moisture content	897:912	moisture content	897:912	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	index					877:881	whitish index	869:881	whitish index	869:881	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	values					825:830	break values	819:830	break values	819:830	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	44	from	increases	771:779	arg1	strength					792:799	tensile strength	784:799	tensile strength	784:799	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	45	theme	moisture	897:904	arg1	content					906:912	moisture content	897:912	moisture content	897:912	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	5	46	theme	maximum	833:839	arg1	temperatures					855:866	maximum decomposition temperatures	833:866	maximum decomposition temperatures	833:866	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	7	47	theme	potential	1382:1390	arg1	value					1392:1396	the potential value	1378:1396	the potential value as biodegradable and edible biomaterials for food packaging	1378:1456	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	3	48	theme	reducing	584:591	arg1	power					593:597	reducing power	584:597	reducing power	584:597	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power were investigated.
31816372	5	49	from	temperatures	855:866	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	6	50	theme	DPPH	1191:1194	arg1	effect					1215:1220	the stronger DPPH radical scavenging effect	1178:1220	the stronger DPPH radical scavenging effect	1178:1220	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	0	51	theme	chitosan	58:65	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and physicochemical properties of antioxidant chitosan ascorbate/methylcellulose composite films.
31816372	0	51	theme	chitosan	58:65	arg1	properties					32:41	physicochemical properties	16:41	physicochemical properties	16:41	Preparation and physicochemical properties of antioxidant chitosan ascorbate/methylcellulose composite films.
31816372	7	52	theme	strong	1345:1350	arg1	action					1364:1369	strong antioxidant action	1345:1369	strong antioxidant action	1345:1369	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	6	53	theme	scavenging	1204:1213	arg1	effect					1215:1220	the stronger DPPH radical scavenging effect	1178:1220	the stronger DPPH radical scavenging effect	1178:1220	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	3	54	theme	physicochemical	506:520	arg1	properties					522:531	Their physicochemical properties	500:531	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power	500:597	Their physicochemical properties as well as the DPPH radical scavenging ability and reducing power were investigated.
31816372	2	55	theme	different	395:403	arg1	ratios					405:410	different ratios	395:410	different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique	395:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	7	56	with	films	1291:1295	arg1	properties					1330:1339	interesting physicochemical properties	1302:1339	interesting physicochemical properties	1302:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	2	57	theme	composite	312:320	arg1	films					322:326	edible composite films	305:326	edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique	305:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	5	58	from	compactness	884:894	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	2	59	dep	ratios	405:410	arg1	1:1					428:430	1:1	428:430	1:1	428:430	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	0:1					447:449	0:1	447:449	0:1	447:449	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	1,0					413:415	1,0	413:415	1,0	413:415	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	4:1					418:420	4:1	418:420	4:1	418:420	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	1:2					433:435	1:2	433:435	1:2	433:435	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	1:4					438:440	1:4	438:440	1:4	438:440	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	59	dep	ratios	405:410	arg1	2:1					423:425	2:1	423:425	2:1	423:425	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	6	60	theme	greater	1072:1078	arg1	proportion					1080:1089	a greater proportion	1070:1089	a greater proportion of chitosan ascorbate	1070:1111	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	2	61	theme	films	322:326	arg1	class					296:300	a class	294:300	a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique	294:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	2	62	theme	edible	305:310	arg1	films					322:326	edible composite films	305:326	edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique	305:497	Here, we reported a class of edible composite films based on chitosan ascorbate and methylcellulose prepared by mixing different ratios (1,0, 4:1, 2:1, 1:1, 1:2, 1:4, and 0:1) of the biopolymers using the casting technique.
31816372	1	63	theme	promising	179:187	arg1	films					145:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films	110:149	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	1	63	theme	promising	179:187	arg1	candidates					189:198	the promising candidates	175:198	the promising candidates for food packaging industry instead of petroleum-based packaging materials	175:273	Polysaccharide-based biodegradable films have been considered as the promising candidates for food packaging industry instead of petroleum-based packaging materials.
31816372	7	64	theme	physicochemical	1314:1328	arg1	properties					1330:1339	interesting physicochemical properties	1302:1339	interesting physicochemical properties	1302:1339	The chitosan ascorbate/methylcellulose composite films with interesting physicochemical properties and strong antioxidant action showed the potential value as biodegradable and edible biomaterials for food packaging.
31816372	5	65	from	elongation	805:814	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	4	66	theme	antioxidant	653:663	arg1	activities					665:674	antioxidant activities	653:674	antioxidant activities	653:674	All physicochemical properties and antioxidant activities were significantly affected by the chitosan ascorbate/methylcellulose ratio in the matrix.
31816372	4	67	theme	ascorbate/methylcellulose	720:744	arg1	ratio					746:750	the chitosan ascorbate/methylcellulose ratio	707:750	the chitosan ascorbate/methylcellulose ratio in the matrix	707:764	All physicochemical properties and antioxidant activities were significantly affected by the chitosan ascorbate/methylcellulose ratio in the matrix.
31816372	0	68	theme	composite	93:101	arg1	films					103:107	composite films	93:107	composite films	93:107	Preparation and physicochemical properties of antioxidant chitosan ascorbate/methylcellulose composite films.
31816372	6	69	theme	radical	1196:1202	arg1	effect					1215:1220	the stronger DPPH radical scavenging effect	1178:1220	the stronger DPPH radical scavenging effect	1178:1220	But the composite films containing a greater proportion of chitosan ascorbate exhibited the better barrier properties against UV-vis light and the stronger DPPH radical scavenging effect and reducing power.
31816372	5	70	from	content	906:912	arg1	permeability					946:957	water vapor permeability	934:957	water vapor permeability	934:957	The increases in tensile strength and elongation at break values, maximum decomposition temperatures, whitish index, compactness, moisture content, and a reduction in water vapor permeability were observed as the proportion of methylcellulose increased in the matrix.
31816372	4	71	theme	chitosan	711:718	arg1	ratio					746:750	the chitosan ascorbate/methylcellulose ratio	707:750	the chitosan ascorbate/methylcellulose ratio in the matrix	707:764	All physicochemical properties and antioxidant activities were significantly affected by the chitosan ascorbate/methylcellulose ratio in the matrix.
31816372	4	72	theme	physicochemical	622:636	arg1	properties					638:647	All physicochemical properties	618:647	All physicochemical properties	618:647	All physicochemical properties and antioxidant activities were significantly affected by the chitosan ascorbate/methylcellulose ratio in the matrix.
29628263	0	0	theme	marine	95:100	arg1	sinuosae					120:127	the marine fungus Hansfordia sinuosae	91:127	the marine fungus Hansfordia sinuosae	91:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	6	1	theme	carcinoma	785:793	arg1	cells					800:804	human cervical carcinoma HeLa cells	770:804	human cervical carcinoma HeLa cells	770:804	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	4	2	theme	2D	491:492	arg1	NMR					494:496	2D NMR	491:496	2D NMR	491:496	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	6	3	theme	remarkable	738:747	arg1	effect					760:765	remarkable inhibitory effect	738:765	remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells	738:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	9	4	theme	antitumor	1229:1237	arg1	agent					1239:1243	a potential antitumor agent	1217:1243	a potential antitumor agent	1217:1243	The results suggested that HPA could be explored as a potential antitumor agent.
29628263	9	4	theme	antitumor	1229:1237	arg1	HPA					1192:1194	HPA	1192:1194	HPA	1192:1194	The results suggested that HPA could be explored as a potential antitumor agent.
29628263	0	5	from	characterization	10:25	arg1	sinuosae					120:127	the marine fungus Hansfordia sinuosae	91:127	the marine fungus Hansfordia sinuosae	91:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	4	6	with	-α-d-Manp	595:603	arg1	1→					624:625	1→	624:625	1→	624:625	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	6	with	-α-d-Manp	595:603	arg1	[α-d-Manp					614:622	[α-d-Manp	614:622	[α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→]	614:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	0	7	theme	Hansfordia	109:118	arg1	sinuosae					120:127	the marine fungus Hansfordia sinuosae	91:127	the marine fungus Hansfordia sinuosae	91:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	6	8	theme	human	770:774	arg1	cells					800:804	human cervical carcinoma HeLa cells	770:804	human cervical carcinoma HeLa cells	770:804	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	0	9	theme	fungus	102:107	arg1	sinuosae					120:127	the marine fungus Hansfordia sinuosae	91:127	the marine fungus Hansfordia sinuosae	91:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	8	10	theme	caspase-3	1154:1162	arg1	expression					1140:1149	the expression	1136:1149	the expression of caspase-3	1136:1162	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	2	11	theme	galactose	340:348	arg1	glucose					354:360	glucose	354:360	glucose	354:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	11	theme	galactose	340:348	arg1	galactose					340:348	galactose	340:348	galactose	340:348	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	11	theme	galactose	340:348	arg1	amounts					329:335	minor amounts	323:335	minor amounts of galactose and glucose	323:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	8	12	theme	HeLa	1000:1003	arg1	cells					1005:1009	HeLa cells	1000:1009	HeLa cells	1000:1009	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	4	13	theme	-α-d-Manp	660:668	arg1	1→					670:671	[→2,6)-α-d-Manp(1→]	654:672	[→2,6)-α-d-Manp(1→]	654:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	3	14	theme	molecular	367:375	arg1	22.5 kDa					409:416	22.5 kDa	409:416	22.5 kDa	409:416	The molecular weight of HPA was approximately 22.5 kDa as analyzed by HPGPC.
29628263	3	14	theme	molecular	367:375	arg1	weight					377:382	The molecular weight	363:382	The molecular weight of HPA	363:389	The molecular weight of HPA was approximately 22.5 kDa as analyzed by HPGPC.
29628263	6	15	theme	inhibitory	749:758	arg1	effect					760:765	remarkable inhibitory effect	738:765	remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells	738:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	5	16	theme	antitumor	679:687	arg1	effect					689:694	The antitumor effect	675:694	The antitumor effect of HPA	675:701	The antitumor effect of HPA was evaluated in vitro.
29628263	0	17	from	activity	41:48	arg1	sinuosae					120:127	the marine fungus Hansfordia sinuosae	91:127	the marine fungus Hansfordia sinuosae	91:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	6	18	theme	cervical	776:783	arg1	cells					800:804	human cervical carcinoma HeLa cells	770:804	human cervical carcinoma HeLa cells	770:804	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	0	19	theme	Structure	0:8	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	0	20	theme	antitumor	31:39	arg1	activity					41:48	antitumor activity	31:48	antitumor activity	31:48	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	4	21	with	[α-d-Manp	533:541	arg1	1→					624:625	1→	624:625	1→	624:625	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	21	with	[α-d-Manp	533:541	arg1	[α-d-Manp					614:622	[α-d-Manp	614:622	[α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→]	614:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	22	with	HPA	462:464	arg1	1D					487:488	1D	487:488	1D	487:488	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	22	with	HPA	462:464	arg1	NMR					494:496	2D NMR	491:496	2D NMR	491:496	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	22	with	HPA	462:464	arg1	methylation					471:481	methylation	471:481	methylation	471:481	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	3	23	theme	HPA	387:389	arg1	22.5 kDa					409:416	22.5 kDa	409:416	22.5 kDa	409:416	The molecular weight of HPA was approximately 22.5 kDa as analyzed by HPGPC.
29628263	3	23	theme	HPA	387:389	arg1	weight					377:382	The molecular weight	363:382	The molecular weight of HPA	363:389	The molecular weight of HPA was approximately 22.5 kDa as analyzed by HPGPC.
29628263	2	24	theme	minor	323:327	arg1	glucose					354:360	glucose	354:360	glucose	354:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	24	theme	minor	323:327	arg1	galactose					340:348	galactose	340:348	galactose	340:348	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	24	theme	minor	323:327	arg1	amounts					329:335	minor amounts	323:335	minor amounts of galactose and glucose	323:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	1	25	theme	marine	197:202	arg1	sinuosae					222:229	the marine fungus Hansfordia sinuosae	193:229	the marine fungus Hansfordia sinuosae	193:229	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	6	26	theme	MCF-7	833:837	arg1	cells					839:843	human breast carcinoma MCF-7 cells	810:843	human breast carcinoma MCF-7 cells	810:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	7	27	theme	HeLa	927:930	arg1	cells					942:946	HeLa and MCF-7 cells	927:946	HeLa and MCF-7 cells	927:946	When cells were incubated with HPA at 400 μg/mL for 48 h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively.
29628263	1	28	attach	isolated	179:186	arg2	HPA					170:172	HPA	170:172	HPA	170:172	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	1	28	attach	isolated	179:186	arg2	polysaccharide					154:167	A neutral water soluble polysaccharide	130:167	A neutral water soluble polysaccharide (HPA)	130:173	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	1	28	attach	isolated	179:186	arg1	sinuosae					222:229	the marine fungus Hansfordia sinuosae	193:229	the marine fungus Hansfordia sinuosae	193:229	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	4	29	theme	C-6	638:640	arg1	position					642:649	C-6 position	638:649	C-6 position of [→2,6)-α-d-Manp(1→]	638:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	1	30	theme	fungus	204:209	arg1	sinuosae					222:229	the marine fungus Hansfordia sinuosae	193:229	the marine fungus Hansfordia sinuosae	193:229	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	8	31	theme	intracellular	1031:1043	arg1	levels					1049:1054	intracellular ROS levels	1031:1054	intracellular ROS levels	1031:1054	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	2	32	theme	glucose	354:360	arg1	glucose					354:360	glucose	354:360	glucose	354:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	32	theme	glucose	354:360	arg1	galactose					340:348	galactose	340:348	galactose	340:348	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	2	32	theme	glucose	354:360	arg1	amounts					329:335	minor amounts	323:335	minor amounts of galactose and glucose	323:360	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	7	33	theme	inhibition	908:917	arg1	rate					919:922	the inhibition rate	904:922	the inhibition rate on HeLa and MCF-7 cells	904:946	When cells were incubated with HPA at 400 μg/mL for 48 h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively.
29628263	7	33	theme	inhibition	908:917	arg1	%					956:956	79.5%	952:956	79.5%	952:956	When cells were incubated with HPA at 400 μg/mL for 48 h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively.
29628263	4	34	theme	HPA	462:464	arg1	analysis					450:457	Structure analysis	440:457	Structure analysis of HPA with methylation and 1D, 2D NMR	440:496	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	6	35	theme	carcinoma	823:831	arg1	cells					839:843	human breast carcinoma MCF-7 cells	810:843	human breast carcinoma MCF-7 cells	810:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	8	36	theme	cells	1064:1068	arg1	apoptosis					1070:1078	cells apoptosis	1064:1078	cells apoptosis	1064:1078	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	1	37	theme	Hansfordia	211:220	arg1	sinuosae					222:229	the marine fungus Hansfordia sinuosae	193:229	the marine fungus Hansfordia sinuosae	193:229	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	4	38	attach	linked	628:633	arg2	1→					624:625	1→	624:625	1→	624:625	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	38	attach	linked	628:633	arg1	position					642:649	C-6 position	638:649	C-6 position of [→2,6)-α-d-Manp(1→]	638:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	38	attach	linked	628:633	arg2	[α-d-Manp					614:622	[α-d-Manp	614:622	[α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→]	614:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	7	39	from	rate	919:922	arg1	cells					942:946	HeLa and MCF-7 cells	927:946	HeLa and MCF-7 cells	927:946	When cells were incubated with HPA at 400 μg/mL for 48 h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively.
29628263	8	40	theme	membrane	1104:1111	arg1	potential					1113:1121	mitochondrial membrane potential	1090:1121	mitochondrial membrane potential	1090:1121	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	2	41	theme	composition	247:257	arg1	analysis					259:266	Monosaccharide composition analysis	232:266	Monosaccharide composition analysis	232:266	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	6	42	from	effect	760:765	arg1	cells					800:804	human cervical carcinoma HeLa cells	770:804	human cervical carcinoma HeLa cells	770:804	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	6	42	from	effect	760:765	arg1	cells					839:843	human breast carcinoma MCF-7 cells	810:843	human breast carcinoma MCF-7 cells	810:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	0	43	theme	extracellular	57:69	arg1	polysaccharide					71:84	the extracellular polysaccharide	53:84	the extracellular polysaccharide from the marine fungus Hansfordia sinuosae	53:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	6	44	theme	breast	816:821	arg1	cells					839:843	human breast carcinoma MCF-7 cells	810:843	human breast carcinoma MCF-7 cells	810:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	2	45	theme	Monosaccharide	232:245	arg1	analysis					259:266	Monosaccharide composition analysis	232:266	Monosaccharide composition analysis	232:266	Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose.
29628263	8	46	theme	ROS	1045:1047	arg1	levels					1049:1054	intracellular ROS levels	1031:1054	intracellular ROS levels	1031:1054	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	4	47	theme	Structure	440:448	arg1	analysis					450:457	Structure analysis	440:457	Structure analysis of HPA with methylation and 1D, 2D NMR	440:496	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	1	48	theme	neutral	132:138	arg1	HPA					170:172	HPA	170:172	HPA	170:172	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	1	48	theme	neutral	132:138	arg1	polysaccharide					154:167	A neutral water soluble polysaccharide	130:167	A neutral water soluble polysaccharide (HPA)	130:173	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	0	49	from	sinuosae	120:127	arg1	polysaccharide					71:84	the extracellular polysaccharide	53:84	the extracellular polysaccharide from the marine fungus Hansfordia sinuosae	53:127	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	0	49	from	sinuosae	120:127	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	0	49	from	sinuosae	120:127	arg1	activity					41:48	antitumor activity	31:48	antitumor activity	31:48	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	4	50	theme	1→	670:671	arg1	position					642:649	C-6 position	638:649	C-6 position of [→2,6)-α-d-Manp(1→]	638:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	8	51	theme	mitochondrial	1090:1102	arg1	potential					1113:1121	mitochondrial membrane potential	1090:1121	mitochondrial membrane potential	1090:1121	Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3.
29628263	1	52	theme	soluble	146:152	arg1	HPA					170:172	HPA	170:172	HPA	170:172	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	1	52	theme	soluble	146:152	arg1	polysaccharide					154:167	A neutral water soluble polysaccharide	130:167	A neutral water soluble polysaccharide (HPA)	130:173	A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae.
29628263	7	53	theme	MCF-7	936:940	arg1	cells					942:946	HeLa and MCF-7 cells	927:946	HeLa and MCF-7 cells	927:946	When cells were incubated with HPA at 400 μg/mL for 48 h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively.
29628263	4	54	with	-α-d-Manp	571:579	arg1	1→					624:625	1→	624:625	1→	624:625	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	54	with	-α-d-Manp	571:579	arg1	[α-d-Manp					614:622	[α-d-Manp	614:622	[α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→]	614:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	55	with	-α-d-Manp	552:560	arg1	1→					624:625	1→	624:625	1→	624:625	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	4	55	with	-α-d-Manp	552:560	arg1	[α-d-Manp					614:622	[α-d-Manp	614:622	[α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→]	614:672	Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1→], [→2)-α-d-Manp(1→], [→3)-α-d-Manp(1→] and [→2,6)-α-d-Manp(1→] with [α-d-Manp(1→] linked to C-6 position of [→2,6)-α-d-Manp(1→].
29628263	5	56	theme	HPA	699:701	arg1	effect					689:694	The antitumor effect	675:694	The antitumor effect of HPA	675:701	The antitumor effect of HPA was evaluated in vitro.
29628263	0	57	theme	polysaccharide	71:84	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	0	57	theme	polysaccharide	71:84	arg1	activity					41:48	antitumor activity	31:48	antitumor activity	31:48	Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.
29628263	6	58	theme	HeLa	795:798	arg1	cells					800:804	human cervical carcinoma HeLa cells	770:804	human cervical carcinoma HeLa cells	770:804	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29628263	9	59	theme	potential	1219:1227	arg1	agent					1239:1243	a potential antitumor agent	1217:1243	a potential antitumor agent	1217:1243	The results suggested that HPA could be explored as a potential antitumor agent.
29628263	9	59	theme	potential	1219:1227	arg1	HPA					1192:1194	HPA	1192:1194	HPA	1192:1194	The results suggested that HPA could be explored as a potential antitumor agent.
29628263	6	60	theme	human	810:814	arg1	cells					839:843	human breast carcinoma MCF-7 cells	810:843	human breast carcinoma MCF-7 cells	810:843	HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells.
29751142	0	0	theme	chitosan	75:82	arg1	oligosaccharide					84:98	parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	38:98	parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	38:98	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	5	1	theme	structural	863:872	arg1	integrity					874:882	The structural integrity	859:882	The structural integrity of coprecipitated PTH(1-34)	859:910	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	1	2	theme	supercritical	267:279	arg1	atomization					296:306	supercritical fluid assisted atomization	267:306	supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM)	267:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	0	3	theme	-loaded	67:73	arg1	oligosaccharide					84:98	parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	38:98	parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	38:98	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	2	4	theme	chitosan	373:380	arg1	oligosaccharide					382:396	chitosan oligosaccharide	373:396	chitosan oligosaccharide (CSO)	373:402	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	2	4	theme	chitosan	373:380	arg1	carrier					422:428	a carrier	420:428	a carrier in an effort to enhance transmucosal absorption of the drug	420:488	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	2	4	theme	chitosan	373:380	arg1	CSO					399:401	CSO	399:401	CSO	399:401	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	1	5	theme	fluid	281:285	arg1	atomization					296:306	supercritical fluid assisted atomization	267:306	supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM)	267:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	7	6	theme	SAA-HCM	1141:1147	arg1	process					1149:1155	The SAA-HCM process	1137:1155	The SAA-HCM process	1137:1155	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	7	6	theme	SAA-HCM	1141:1147	arg1	technique					1180:1188	a green technique	1172:1188	a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents	1172:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	7	7	theme	organic	1276:1282	arg1	solvents					1284:1291	any organic solvents	1272:1291	any organic solvents	1272:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	5	8	theme	high	971:974	arg1	efficiency					984:993	a high loading efficiency	969:993	a high loading efficiency up to 92.8%	969:1005	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	3	9	theme	/CSO	538:541	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	4	10	theme	mass	742:745	arg1	diameter					766:773	the mass median aerodynamic diameter	738:773	the mass median aerodynamic diameter (MMAD)	738:780	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	10	theme	mass	742:745	arg1	MMAD					776:779	MMAD	776:779	MMAD	776:779	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	1	11	theme	assisted	287:294	arg1	atomization					296:306	supercritical fluid assisted atomization	267:306	supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM)	267:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	0	12	theme	oligosaccharide	84:98	arg1	powders					27:33	micrometric powders	15:33	micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	15:98	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	4	13	theme	aerodynamic	754:764	arg1	diameter					766:773	the mass median aerodynamic diameter	738:773	the mass median aerodynamic diameter (MMAD)	738:780	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	13	theme	aerodynamic	754:764	arg1	MMAD					776:779	MMAD	776:779	MMAD	776:779	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	3	14	theme	composite	543:551	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	3	15	theme	Well-defined	491:502	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	0	16	theme	fluid	117:121	arg1	atomization					132:142	supercritical fluid assisted atomization	103:142	supercritical fluid assisted atomization	103:142	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	1	17	theme	-loaded	174:180	arg1	powders					186:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	0	18	theme	supercritical	103:115	arg1	atomization					132:142	supercritical fluid assisted atomization	103:142	supercritical fluid assisted atomization	103:142	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	2	19	theme	drug	485:488	arg1	absorption					467:476	transmucosal absorption	454:476	transmucosal absorption of the drug	454:488	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	6	20	theme	amorphous	1103:1111	arg1	structure					1113:1121	amorphous structure	1103:1121	amorphous structure of particles	1103:1134	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	6	21	theme	TGA	1021:1023	arg1	analyses					1025:1032	TGA analyses	1021:1032	TGA analyses	1021:1032	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	1	22	theme	dry	182:184	arg1	powders					186:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	2	23	theme	transmucosal	454:465	arg1	absorption					467:476	transmucosal absorption	454:476	transmucosal absorption of the drug	454:488	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	6	24	dep	revealed	1034:1041	arg1	preserved					1069:1077	preserved	1069:1077	revealed its thermal stability was preserved	1034:1077	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	7	25	theme	inhalable	1209:1217	arg1	powders					1249:1255	inhalable protein/polymer composite dry powders	1209:1255	inhalable protein/polymer composite dry powders without use of any organic solvents	1209:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	0	26	dep	-loaded	67:73	arg1	hormone					50:56	parathyroid hormone	38:56	parathyroid hormone	38:56	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	4	27	theme	generation	707:716	arg1	NGI					728:730	NGI	728:730	NGI	728:730	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	27	theme	generation	707:716	arg1	impactor					718:725	next generation impactor	702:725	next generation impactor (NGI)	702:731	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	0	28	theme	powders	27:33	arg1	Preparation					0:10	Preparation	0:10	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.	0:143	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	5	29	theme	loading	976:982	arg1	efficiency					984:993	a high loading efficiency	969:993	a high loading efficiency up to 92.8%	969:1005	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	4	30	theme	median	747:752	arg1	diameter					766:773	the mass median aerodynamic diameter	738:773	the mass median aerodynamic diameter (MMAD)	738:780	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	30	theme	median	747:752	arg1	MMAD					776:779	MMAD	776:779	MMAD	776:779	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	0	31	theme	micrometric	15:25	arg1	powders					27:33	micrometric powders	15:33	micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide	15:98	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	0	32	theme	assisted	123:130	arg1	atomization					132:142	supercritical fluid assisted atomization	103:142	supercritical fluid assisted atomization	103:142	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	6	33	theme	particles	1126:1134	arg1	structure					1113:1121	amorphous structure	1103:1121	amorphous structure of particles	1103:1134	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	3	34	theme	spherical	519:527	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	3	35	theme	PTH	529:531	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	0	36	theme	parathyroid	38:48	arg1	hormone					50:56	parathyroid hormone	38:56	parathyroid hormone	38:56	Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization.
29751142	1	37	theme	hydrodynamic	324:335	arg1	mixer					348:352	a hydrodynamic cavitation mixer	322:352	a hydrodynamic cavitation mixer (SAA-HCM)	322:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	1	37	theme	hydrodynamic	324:335	arg1	SAA-HCM					355:361	SAA-HCM	355:361	SAA-HCM	355:361	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	4	38	dep	%	856:856	arg1	63.51					851:855	63.51	851:855	63.51	851:855	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	7	39	theme	dry	1245:1247	arg1	powders					1249:1255	inhalable protein/polymer composite dry powders	1209:1255	inhalable protein/polymer composite dry powders without use of any organic solvents	1209:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	3	40	theme	particles	591:599	arg1	size					601:604	the particles size	587:604	the particles size	587:604	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	1	41	theme	aqueous	215:221	arg1	solution					223:230	aqueous solution	215:230	aqueous solution	215:230	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	4	42	theme	next	702:705	arg1	NGI					728:730	NGI	728:730	NGI	728:730	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	42	theme	next	702:705	arg1	impactor					718:725	next generation impactor	702:725	next generation impactor (NGI)	702:731	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	1	43	theme	cavitation	337:346	arg1	mixer					348:352	a hydrodynamic cavitation mixer	322:352	a hydrodynamic cavitation mixer (SAA-HCM)	322:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	1	43	theme	cavitation	337:346	arg1	SAA-HCM					355:361	SAA-HCM	355:361	SAA-HCM	355:361	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	4	44	dep	63.51	851:855	arg1	to					848:849	to	848:849	to	848:849	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	5	45	theme	coprecipitated	887:900	arg1	1-34					906:909	1-34	906:909	1-34	906:909	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	5	45	theme	coprecipitated	887:900	arg1	PTH					902:904	PTH	902:904	coprecipitated PTH(1-34)	887:910	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	7	46	theme	powders	1249:1255	arg1	preparation					1194:1204	preparation	1194:1204	preparation of inhalable protein/polymer composite dry powders without use of any organic solvents	1194:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	3	47	theme	narrow	636:641	arg1	distribution					643:654	narrow distribution	636:654	narrow distribution	636:654	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	4	48	theme	particle	821:828	arg1	FPF					840:842	FPF	840:842	FPF	840:842	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	48	theme	particle	821:828	arg1	fraction					830:837	fine particle fraction	816:837	fine particle fraction (FPF) up to 63.51%	816:856	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	7	49	theme	protein/polymer	1219:1233	arg1	powders					1249:1255	inhalable protein/polymer composite dry powders	1209:1255	inhalable protein/polymer composite dry powders without use of any organic solvents	1209:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	4	50	theme	fine	816:819	arg1	FPF					840:842	FPF	840:842	FPF	840:842	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	4	50	theme	fine	816:819	arg1	fraction					830:837	fine particle fraction	816:837	fine particle fraction (FPF) up to 63.51%	816:856	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	7	51	theme	solvents	1284:1291	arg1	use					1265:1267	use	1265:1267	use of any organic solvents	1265:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	3	52	theme	separated	505:513	arg1	microparticles					553:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles	491:566	Well-defined, separated and spherical PTH(1-34)/CSO composite microparticles were obtained, and the particles size could be well controlled with narrow distribution.
29751142	5	53	dep	92.8	1001:1004	arg1	to					998:999	to	998:999	to	998:999	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	5	54	theme	PTH	902:904	arg1	integrity					874:882	The structural integrity	859:882	The structural integrity of coprecipitated PTH(1-34)	859:910	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	6	55	theme	XRD	1083:1085	arg1	patterns					1087:1094	XRD patterns	1083:1094	XRD patterns	1083:1094	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	6	56	theme	thermal	1047:1053	arg1	stability					1055:1063	its thermal stability	1043:1063	its thermal stability	1043:1063	TGA analyses revealed its thermal stability was preserved and XRD patterns showed amorphous structure of particles.
29751142	7	57	theme	composite	1235:1243	arg1	powders					1249:1255	inhalable protein/polymer composite dry powders	1209:1255	inhalable protein/polymer composite dry powders without use of any organic solvents	1209:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	5	58	theme	circular	945:952	arg1	dichroism					954:962	circular dichroism	945:962	circular dichroism	945:962	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	2	59	from	carrier	422:428	arg1	effort					436:441	an effort to enhance transmucosal absorption of the drug	433:488	an effort to enhance transmucosal absorption of the drug	433:488	Herein, chitosan oligosaccharide (CSO) was selected as a carrier in an effort to enhance transmucosal absorption of the drug.
29751142	1	60	theme	Parathyroid	145:155	arg1	PTH1-34					166:172	PTH1-34	166:172	PTH1-34	166:172	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	1	60	theme	Parathyroid	145:155	arg1	hormone					157:163	Parathyroid hormone	145:163	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	1	61	theme	pulmonary	236:244	arg1	administration					246:259	pulmonary administration	236:259	pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM)	236:362	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	4	62	theme	1-5 μm	798:803	arg1	range					805:809	strictly 1-5 μm range	789:809	strictly 1-5 μm range	789:809	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	1	63	theme	hormone	157:163	arg1	powders					186:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders	145:192	Parathyroid hormone (PTH1-34)-loaded dry powders were fabricated from aqueous solution for pulmonary administration using supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM).
29751142	4	64	theme	Aerodynamic	657:667	arg1	performance					669:679	Aerodynamic performance	657:679	Aerodynamic performance	657:679	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	5	65	dep	%	1005:1005	arg1	92.8					1001:1004	92.8	1001:1004	92.8	1001:1004	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	5	66	dep	efficiency	984:993	arg1	%					1005:1005	%	1005:1005	%	1005:1005	The structural integrity of coprecipitated PTH(1-34) was validated by HPLC, FT-IR and circular dichroism, and a high loading efficiency up to 92.8% was obtained.
29751142	4	67	dep	fraction	830:837	arg1	%					856:856	%	856:856	%	856:856	Aerodynamic performance was determined using next generation impactor (NGI), and the mass median aerodynamic diameter (MMAD) ranged strictly 1-5 μm range with fine particle fraction (FPF) up to 63.51%.
29751142	7	68	theme	green	1174:1178	arg1	process					1149:1155	The SAA-HCM process	1137:1155	The SAA-HCM process	1137:1155	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
29751142	7	68	theme	green	1174:1178	arg1	technique					1180:1188	a green technique	1172:1188	a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents	1172:1291	The SAA-HCM process is proposed as a green technique for preparation of inhalable protein/polymer composite dry powders without use of any organic solvents.
30615421	6	0	theme	fire	1211:1214	arg1	tests					1229:1233	fire breakthrough tests	1211:1233	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests	1073:1233	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	0	theme	fire	1211:1214	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	0	theme	fire	1211:1214	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	8	1	theme	barrier	1822:1828	arg1	properties					1830:1839	extraordinary flame-retardant and fire barrier properties	1783:1839	extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1783:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	8	2	theme	room	1898:1901	arg1	temperature					1903:1913	benign water-based room temperature	1879:1913	benign water-based room temperature processing	1879:1924	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	1	3	theme	mechanical	224:233	arg1	performance					235:245	high mechanical performance	219:245	high mechanical performance	219:245	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	1	3	theme	mechanical	224:233	arg1	properties					256:265	barrier properties	248:265	barrier properties	248:265	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	4	4	theme	flame-retardant	957:971	arg1	materials					973:981	metal-free flame-retardant materials	946:981	metal-free flame-retardant materials	946:981	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	7	5	theme	chemical	1575:1582	arg1	modifications					1584:1596	significant chemical modifications	1563:1596	significant chemical modifications	1563:1596	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	5	6	theme	fire	989:992	arg1	nanopapers					1002:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers can even be obtained by hybridizing the ChNF with nanoclay.
30615421	6	7	theme	fire	1087:1090	arg1	tests					1103:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	7	theme	fire	1087:1090	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	7	theme	fire	1087:1090	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	8	theme	high	1286:1289	arg1	integrity					1302:1310	high structural integrity	1286:1310	high structural integrity	1286:1310	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	5	9	with	ChNF	1053:1056	arg1	nanoclay					1063:1070	nanoclay	1063:1070	nanoclay	1063:1070	Full fire barrier nanopapers can even be obtained by hybridizing the ChNF with nanoclay.
30615421	3	10	theme	phosphate	643:651	arg1	type					653:656	the phosphate type	639:656	the phosphate type	639:656	We show that the flammability can be critically reduced by exchanging the counterions, e.g. to the phosphate type, using the respective acid providing electrostatic stabilization in the preparation of the ChNFs.
30615421	8	11	theme	halogen	1960:1966	arg1	atoms					1968:1972	halogen atoms	1960:1972	halogen atoms	1960:1972	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	7	12	theme	nanocellulose	1520:1532	arg1	materials					1534:1542	related, but flammable nanocellulose materials	1497:1542	related, but flammable nanocellulose materials	1497:1542	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	4	13	theme	high	815:818	arg1	contents					820:827	high contents	815:827	high contents of N/P (nitrogen/phosphorus)	815:856	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	4	14	from	performance	912:922	arg1	heavy					940:944	heavy	940:944	heavy	940:944	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	4	14	from	performance	912:922	arg1	halogen-					927:934	halogen-	927:934	halogen-	927:934	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	6	15	theme	flame	1144:1148	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	8	16	theme	food	1661:1664	arg1	waste					1666:1670	a global food waste	1652:1670	a global food waste	1652:1670	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	7	17	theme	related	1497:1503	arg1	materials					1534:1542	related, but flammable nanocellulose materials	1497:1542	related, but flammable nanocellulose materials	1497:1542	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	4	18	theme	beneficial	778:787	arg1	combinations					799:810	beneficial elemental combinations	778:810	beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus)	778:856	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	3	19	theme	electrostatic	695:707	arg1	stabilization					709:721	electrostatic stabilization	695:721	electrostatic stabilization	695:721	We show that the flammability can be critically reduced by exchanging the counterions, e.g. to the phosphate type, using the respective acid providing electrostatic stabilization in the preparation of the ChNFs.
30615421	1	20	theme	advanced	190:197	arg1	materials					199:207	advanced materials	190:207	advanced materials requiring high mechanical performance, barrier properties, for bioactive materials	190:290	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	7	21	theme	chitin	1370:1375	arg1	modification					1414:1425	the simple modification	1403:1425	the simple modification of the counterions to include phosphorus	1403:1466	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	7	21	theme	chitin	1370:1375	arg1	composition					1355:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	1	22	theme	polysaccharide	91:104	arg1	nanofibrils					106:116	Sustainable polysaccharide nanofibrils	79:116	Sustainable polysaccharide nanofibrils formed from chitin or cellulose	79:148	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	7	23	theme	intrinsic	1335:1343	arg1	composition					1355:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	0	24	theme	Sustainable	0:10	arg1	Nanofibrils					19:29	Sustainable Chitin Nanofibrils	0:29	Sustainable Chitin Nanofibrils	0:29	Sustainable Chitin Nanofibrils Provide Outstanding Flame-Retardant Nanopapers.
30615421	7	25	theme	key	1477:1479	arg1	advantages					1481:1490	key advantages	1477:1490	key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant	1477:1635	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	8	26	theme	bioinspired	1716:1726	arg1	materials					1768:1776	bioinspired, green, and mechanical high-performance materials	1716:1776	bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1716:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	2	27	dep	flame-retardant	400:414	arg1	mechanical					440:449	mechanical	440:449	mechanical	440:449	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	6	28	theme	cone	1171:1174	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	8	29	theme	green	1729:1733	arg1	materials					1768:1776	bioinspired, green, and mechanical high-performance materials	1716:1776	bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1716:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	6	30	theme	microscale	1160:1169	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	2	31	theme	surface-deacetylated	495:514	arg1	ChNFs					536:540	ChNFs	536:540	ChNFs	536:540	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	2	31	theme	surface-deacetylated	495:514	arg1	nanofibrils					523:533	surface-deacetylated chitin nanofibrils	495:533	surface-deacetylated chitin nanofibrils (ChNFs)	495:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	0	32	theme	Outstanding	39:49	arg1	Nanopapers					67:76	Outstanding Flame-Retardant Nanopapers	39:76	Outstanding Flame-Retardant Nanopapers	39:76	Sustainable Chitin Nanofibrils Provide Outstanding Flame-Retardant Nanopapers.
30615421	2	33	theme	flame-retardant	400:414	arg1	nanopapers					468:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers	386:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs)	386:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	6	34	theme	flame-retardant	1255:1269	arg1	properties					1271:1280	excellent flame-retardant properties	1245:1280	excellent flame-retardant properties	1245:1280	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	8	35	theme	high-performance	1751:1766	arg1	materials					1768:1776	bioinspired, green, and mechanical high-performance materials	1716:1776	bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1716:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	1	36	theme	bioactive	272:280	arg1	materials					282:290	bioactive materials	272:290	bioactive materials	272:290	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	8	37	dep	critical	1695:1702	arg1	advance					1704:1710	advance	1704:1710	advance	1704:1710	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	6	38	theme	breakthrough	1216:1227	arg1	tests					1229:1233	fire breakthrough tests	1211:1233	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests	1073:1233	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	38	theme	breakthrough	1216:1227	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	38	theme	breakthrough	1216:1227	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	8	39	theme	flame-retardant	1797:1811	arg1	properties					1830:1839	extraordinary flame-retardant and fire barrier properties	1783:1839	extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1783:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	2	40	theme	self-extinguishing	420:437	arg1	nanopapers					468:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers	386:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs)	386:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	1	41	theme	other	296:300	arg1	functionalities					302:316	other functionalities	296:316	other functionalities	296:316	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	8	42	theme	fire	1817:1820	arg1	properties					1830:1839	extraordinary flame-retardant and fire barrier properties	1783:1839	extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1783:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	8	43	theme	water-based	1886:1896	arg1	temperature					1903:1913	benign water-based room temperature	1879:1913	benign water-based room temperature processing	1879:1924	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	8	44	theme	temperature	1903:1913	arg1	processing					1915:1924	benign water-based room temperature processing	1879:1924	benign water-based room temperature processing	1879:1924	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	1	45	theme	high	219:222	arg1	performance					235:245	high mechanical performance	219:245	high mechanical performance	219:245	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	1	45	theme	high	219:222	arg1	properties					256:265	barrier properties	248:265	barrier properties	248:265	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	5	46	theme	Full	984:987	arg1	nanopapers					1002:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers can even be obtained by hybridizing the ChNF with nanoclay.
30615421	6	47	theme	retardancy	1092:1101	arg1	tests					1103:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	47	theme	retardancy	1092:1101	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	47	theme	retardancy	1092:1101	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	4	48	theme	metal-free	946:955	arg1	materials					973:981	metal-free flame-retardant materials	946:981	metal-free flame-retardant materials	946:981	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	6	49	theme	structural	1291:1300	arg1	integrity					1302:1310	high structural integrity	1286:1310	high structural integrity	1286:1310	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	1	50	theme	barrier	248:254	arg1	performance					235:245	high mechanical performance	219:245	high mechanical performance	219:245	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	1	50	theme	barrier	248:254	arg1	properties					256:265	barrier properties	248:265	barrier properties	248:265	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	6	51	theme	Comprehensive	1073:1085	arg1	tests					1103:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests	1073:1107	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	51	theme	Comprehensive	1073:1085	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	51	theme	Comprehensive	1073:1085	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	7	52	theme	significant	1563:1573	arg1	modifications					1584:1596	significant chemical modifications	1563:1596	significant chemical modifications	1563:1596	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	3	53	theme	respective	669:678	arg1	acid					680:683	the respective acid	665:683	the respective acid providing electrostatic stabilization in the preparation of the ChNFs	665:753	We show that the flammability can be critically reduced by exchanging the counterions, e.g. to the phosphate type, using the respective acid providing electrostatic stabilization in the preparation of the ChNFs.
30615421	8	54	theme	heavy	1943:1947	arg1	metals					1949:1954	heavy metals	1943:1954	heavy metals	1943:1954	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	8	55	theme	global	1654:1659	arg1	waste					1666:1670	a global food waste	1652:1670	a global food waste	1652:1670	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	2	56	dep	single-step	342:352	arg1	waterborne					355:364	waterborne	355:364	waterborne	355:364	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	8	57	with	materials	1768:1776	arg1	properties					1830:1839	extraordinary flame-retardant and fire barrier properties	1783:1839	extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1783:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	1	58	theme	biobased	163:170	arg1	nanomaterials					172:184	biobased nanomaterials	163:184	biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials	163:290	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	7	59	theme	flammable	1510:1518	arg1	materials					1534:1542	related, but flammable nanocellulose materials	1497:1542	related, but flammable nanocellulose materials	1497:1542	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	4	60	theme	contents	820:827	arg1	combinations					799:810	beneficial elemental combinations	778:810	beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus)	778:856	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	6	61	theme	excellent	1245:1253	arg1	properties					1271:1280	excellent flame-retardant properties	1245:1280	excellent flame-retardant properties	1245:1280	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	4	62	dep	halogen-	927:934	arg1	materials					973:981	metal-free flame-retardant materials	946:981	metal-free flame-retardant materials	946:981	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	5	63	theme	barrier	994:1000	arg1	nanopapers					1002:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers	984:1011	Full fire barrier nanopapers can even be obtained by hybridizing the ChNF with nanoclay.
30615421	6	64	theme	horizontal	1133:1142	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	6	65	theme	vertical	1120:1127	arg1	tests					1150:1154	vertical and horizontal flame tests	1120:1154	vertical and horizontal flame tests	1120:1154	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	4	66	theme	elemental	789:797	arg1	combinations					799:810	beneficial elemental combinations	778:810	beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus)	778:856	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	7	67	theme	elemental	1345:1353	arg1	composition					1355:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	1	68	theme	Sustainable	79:89	arg1	nanofibrils					106:116	Sustainable polysaccharide nanofibrils	79:116	Sustainable polysaccharide nanofibrils formed from chitin or cellulose	79:148	Sustainable polysaccharide nanofibrils formed from chitin or cellulose are emerging biobased nanomaterials for advanced materials requiring high mechanical performance, barrier properties, for bioactive materials, or other functionalities.
30615421	2	69	theme	single-step	342:352	arg1	approach					366:373	a single-step, waterborne approach	340:373	a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs)	340:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	4	70	theme	final	865:869	arg1	nanopapers					871:880	the final nanopapers	861:880	the final nanopapers	861:880	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	0	71	theme	Chitin	12:17	arg1	Nanofibrils					19:29	Sustainable Chitin Nanofibrils	0:29	Sustainable Chitin Nanofibrils	0:29	Sustainable Chitin Nanofibrils Provide Outstanding Flame-Retardant Nanopapers.
30615421	4	72	theme	N/P	832:834	arg1	contents					820:827	high contents	815:827	high contents of N/P (nitrogen/phosphorus)	815:856	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	2	73	theme	high-performance	451:466	arg1	nanopapers					468:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers	386:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs)	386:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	6	74	theme	combustion	1176:1185	arg1	calorimetry					1187:1197	microscale cone combustion calorimetry	1160:1197	microscale cone combustion calorimetry	1160:1197	Comprehensive fire retardancy tests, including vertical and horizontal flame tests and microscale cone combustion calorimetry, as well as fire breakthrough tests elucidate excellent flame-retardant properties and high structural integrity when being burned.
30615421	2	75	theme	chitin	516:521	arg1	ChNFs					536:540	ChNFs	536:540	ChNFs	536:540	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	2	75	theme	chitin	516:521	arg1	nanofibrils					523:533	surface-deacetylated chitin nanofibrils	495:533	surface-deacetylated chitin nanofibrils (ChNFs)	495:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	0	76	theme	Flame-Retardant	51:65	arg1	Nanopapers					67:76	Outstanding Flame-Retardant Nanopapers	39:76	Outstanding Flame-Retardant Nanopapers	39:76	Sustainable Chitin Nanofibrils Provide Outstanding Flame-Retardant Nanopapers.
30615421	7	77	theme	counterions	1434:1444	arg1	modification					1414:1425	the simple modification	1403:1425	the simple modification of the counterions to include phosphorus	1403:1466	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	7	77	theme	counterions	1434:1444	arg1	composition					1355:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	8	78	theme	mechanical	1740:1749	arg1	materials					1768:1776	bioinspired, green, and mechanical high-performance materials	1716:1776	bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1716:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	4	79	theme	outstanding	900:910	arg1	performance					912:922	outstanding performance	900:922	outstanding performance in halogen- and heavy metal-free flame-retardant materials	900:981	This exchange renders beneficial elemental combinations of high contents of N/P (nitrogen/phosphorus) in the final nanopapers, known to provide outstanding performance in halogen- and heavy metal-free flame-retardant materials.
30615421	2	80	theme	additive-free	386:398	arg1	nanopapers					468:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers	386:477	additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs)	386:541	Here, we demonstrate a single-step, waterborne approach to prepare additive-free flame-retardant and self-extinguishing, mechanical high-performance nanopapers based purely on surface-deacetylated chitin nanofibrils (ChNFs).
30615421	8	81	theme	sustainable	1850:1860	arg1	feedstock					1862:1870	sustainable feedstock	1850:1870	sustainable feedstock	1850:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	7	82	theme	simple	1407:1412	arg1	modification					1414:1425	the simple modification	1403:1425	the simple modification of the counterions to include phosphorus	1403:1466	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	8	83	theme	extraordinary	1783:1795	arg1	properties					1830:1839	extraordinary flame-retardant and fire barrier properties	1783:1839	extraordinary flame-retardant and fire barrier properties based on sustainable feedstock	1783:1870	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
30615421	3	84	theme	ChNFs	749:753	arg1	preparation					730:740	the preparation	726:740	the preparation of the ChNFs	726:753	We show that the flammability can be critically reduced by exchanging the counterions, e.g. to the phosphate type, using the respective acid providing electrostatic stabilization in the preparation of the ChNFs.
30615421	7	85	contain	containing	1378:1387	arg2	nitrogen					1389:1396	nitrogen	1389:1396	nitrogen	1389:1396	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	7	85	contain	containing	1378:1387	arg1	composition					1355:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition	1331:1365	The intrinsic elemental composition of chitin, containing nitrogen, and the simple modification of the counterions to include phosphorus provides key advantages over related, but flammable nanocellulose materials that often require significant chemical modifications and additives to become fire-retardant.
30615421	8	86	theme	benign	1879:1884	arg1	temperature					1903:1913	benign water-based room temperature	1879:1913	benign water-based room temperature processing	1879:1924	By activating a global food waste, this study presents a critical advance for bioinspired, green, and mechanical high-performance materials with extraordinary flame-retardant and fire barrier properties based on sustainable feedstock, using benign water-based room temperature processing, and by avoiding heavy metals and halogen atoms in their composition.
31437077	3	0	theme	enhanced	530:537	arg1	localization					539:550	enhanced localization	530:550	enhanced localization	530:550	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	1	1	theme	gel	257:259	arg1	applicability					219:231	the buccal applicability	208:231	the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	208:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	8	2	theme	in	1169:1170	arg1	gel					1187:1189	the prepared in situ composite gel	1156:1189	the prepared in situ composite gel	1156:1189	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	9	3	dep	in	1270:1271	arg1	vitro					1273:1277	vitro	1273:1277	vitro	1273:1277	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	11	4	theme	AC	1550:1551	arg1	delivery					1560:1567	AC buccal delivery	1550:1567	AC buccal delivery	1550:1567	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	1	5	theme	aceclofenac	272:282	arg1	MPs					334:336	MPs	334:336	MPs	334:336	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	5	theme	aceclofenac	272:282	arg1	microparticles					318:331	aceclofenac (AC) amino methacrylate copolymer microparticles	272:331	aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	272:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	8	6	theme	composite	1177:1185	arg1	gel					1187:1189	the prepared in situ composite gel	1156:1189	the prepared in situ composite gel	1156:1189	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	4	7	theme	gel	679:681	arg1	gelation					659:666	gelation	659:666	gelation	659:666	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	4	7	theme	gel	679:681	arg1	crosslinking					642:653	crosslinking	642:653	crosslinking	642:653	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	1	8	theme	investigation	178:190	arg1	aim					159:161	The aim	155:161	The aim of the current investigation	155:190	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	9	9	theme	Sustained	1226:1234	arg1	features					1247:1254	Sustained AC release features	1226:1254	Sustained AC release features	1226:1254	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	3	10	theme	in	600:601	arg1	gel					633:635	in situ ion-activated gellan gum gel	600:635	in situ ion-activated gellan gum gel	600:635	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	10	11	dep	in	1362:1363	arg1	vivo					1365:1368	vivo	1365:1368	vivo	1365:1368	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	8	12	theme	buccal	1206:1211	arg1	suitability					1213:1223	its buccal suitability	1202:1223	its buccal suitability	1202:1223	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	7	13	theme	system	1077:1082	arg1	network					1052:1058	the gel network	1044:1058	the gel network of the composite system	1044:1082	MPs were successfully entrapped inside the gel network of the composite system.
31437077	1	14	theme	conventional	381:392	arg1	therapy					394:400	oral existing conventional therapy	367:400	oral existing conventional therapy	367:400	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	5	15	dep	in	789:790	arg1	vivo					792:795	vivo	792:795	vivo	792:795	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	4	16	theme	in	671:672	arg1	gel					679:681	in situ gel	671:681	in situ gel	671:681	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	5	17	from	Suitability	753:763	arg1	model					834:838	inflammation model	821:838	inflammation model of rats	821:846	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	3	18	theme	gum	629:631	arg1	gel					633:635	in situ ion-activated gellan gum gel	600:635	in situ ion-activated gellan gum gel	600:635	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	6	19	theme	entrapment	966:975	arg1	efficiency					977:986	high entrapment efficiency	961:986	high entrapment efficiency (EE%) of 94.73%	961:1002	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	19	theme	entrapment	966:975	arg1	%					991:991	EE%	989:991	EE%	989:991	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	9	20	theme	in	1270:1271	arg1	response					1294:1301	promising in vitro anti-arthritic response	1260:1301	promising in vitro anti-arthritic response	1260:1301	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	3	21	theme	ion-activated	608:620	arg1	gel					633:635	in situ ion-activated gellan gum gel	600:635	in situ ion-activated gellan gum gel	600:635	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	9	22	theme	anti-arthritic	1279:1292	arg1	response					1294:1301	promising in vitro anti-arthritic response	1260:1301	promising in vitro anti-arthritic response	1260:1301	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	1	23	contain	containing	261:270	arg1	gel					257:259	an in situ composite gel	236:259	an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	236:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	23	contain	containing	261:270	arg2	microparticles					318:331	aceclofenac (AC) amino methacrylate copolymer microparticles	272:331	aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	272:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	23	contain	containing	261:270	arg2	MPs					334:336	MPs	334:336	MPs	334:336	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	4	24	theme	morphological	704:716	arg1	characterizations					734:750	morphological and solid state characterizations	704:750	morphological and solid state characterizations	704:750	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	11	25	theme	gel	1503:1505	arg1	potential					1472:1480	the potential	1468:1480	the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery	1468:1567	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	0	26	theme	amino	61:65	arg1	microparticles					90:103	amino methacrylate copolymer microparticles	61:103	amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability	61:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	4	27	theme	solid	722:726	arg1	characterizations					734:750	morphological and solid state characterizations	704:750	morphological and solid state characterizations	704:750	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	0	28	theme	copolymer	80:88	arg1	microparticles					90:103	amino methacrylate copolymer microparticles	61:103	amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability	61:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	3	29	dep	in	600:601	arg1	situ					603:606	situ	603:606	situ	603:606	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	6	30	theme	%	1002:1002	arg1	size					935:938	particle size	926:938	particle size (PS) of 51.00 µm	926:955	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	30	theme	%	1002:1002	arg1	efficiency					977:986	high entrapment efficiency	961:986	high entrapment efficiency (EE%) of 94.73%	961:1002	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	30	theme	%	1002:1002	arg1	%					991:991	EE%	989:991	EE%	989:991	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	30	theme	%	1002:1002	arg1	PS					941:942	PS	941:942	PS	941:942	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	8	31	dep	in	1169:1170	arg1	situ					1172:1175	situ	1172:1175	situ	1172:1175	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	11	32	theme	therapeutic	1525:1535	arg1	proposal					1537:1544	an appropriate therapeutic proposal	1510:1544	an appropriate therapeutic proposal for AC buccal delivery	1510:1567	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	5	33	theme	buccal	769:774	arg1	delivery					776:783	buccal delivery	769:783	buccal delivery	769:783	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	2	34	theme	AC	403:404	arg1	MPs					421:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs were fabricated and statistically optimized using 2241 factorial design.
31437077	0	35	theme	therapeutic	108:118	arg1	modality					120:127	a therapeutic modality	106:127	amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability	61:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	1	36	theme	oral	367:370	arg1	therapy					394:400	oral existing conventional therapy	367:400	oral existing conventional therapy	367:400	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	0	37	theme	methacrylate	67:78	arg1	microparticles					90:103	amino methacrylate copolymer microparticles	61:103	amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability	61:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	0	38	theme	In	0:1	arg1	gel					43:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	10	39	theme	consistent	1337:1346	arg1	effect					1388:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	0	40	theme	composite	8:16	arg1	gel					43:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	2	41	theme	RL100	415:419	arg1	MPs					421:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs were fabricated and statistically optimized using 2241 factorial design.
31437077	0	42	theme	gellan	32:37	arg1	gel					43:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	1	43	theme	methacrylate	295:306	arg1	MPs					334:336	MPs	334:336	MPs	334:336	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	43	theme	methacrylate	295:306	arg1	microparticles					318:331	aceclofenac (AC) amino methacrylate copolymer microparticles	272:331	aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	272:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	5	44	theme	inflammation	821:832	arg1	model					834:838	inflammation model	821:838	inflammation model of rats	821:846	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	2	45	theme	2241	475:478	arg1	design					490:495	2241 factorial design	475:495	2241 factorial design	475:495	AC Eudragit RL100 MPs were fabricated and statistically optimized using 2241 factorial design.
31437077	3	46	theme	buccal	505:510	arg1	applicability					512:524	Better buccal applicability	498:524	Better buccal applicability	498:524	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	10	47	theme	prolonged	1352:1360	arg1	effect					1388:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	8	48	theme	shear-thinning	1108:1121	arg1	properties					1123:1132	shear-thinning properties	1108:1132	shear-thinning properties	1108:1132	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	1	49	theme	in	239:240	arg1	gel					257:259	an in situ composite gel	236:259	an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	236:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	6	50	theme	51.00 µm	948:955	arg1	size					935:938	particle size	926:938	particle size (PS) of 51.00 µm	926:955	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	50	theme	51.00 µm	948:955	arg1	efficiency					977:986	high entrapment efficiency	961:986	high entrapment efficiency (EE%) of 94.73%	961:1002	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	50	theme	51.00 µm	948:955	arg1	%					991:991	EE%	989:991	EE%	989:991	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	50	theme	51.00 µm	948:955	arg1	PS					941:942	PS	941:942	PS	941:942	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	0	51	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	4	52	dep	crosslinking	642:653	arg1	The					638:640	The	638:640	The	638:640	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	1	53	theme	composite	247:255	arg1	gel					257:259	an in situ composite gel	236:259	an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	236:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	10	54	theme	standard	1421:1428	arg1	AC					1430:1431	standard AC	1421:1431	standard AC	1421:1431	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	8	55	theme	prepared	1160:1167	arg1	gel					1187:1189	the prepared in situ composite gel	1156:1189	the prepared in situ composite gel	1156:1189	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	11	56	theme	buccal	1553:1558	arg1	delivery					1560:1567	AC buccal delivery	1550:1567	AC buccal delivery	1550:1567	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	1	57	theme	current	170:176	arg1	investigation					178:190	the current investigation	166:190	the current investigation	166:190	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	6	58	theme	particle	926:933	arg1	size					935:938	particle size	926:938	particle size (PS) of 51.00 µm	926:955	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	58	theme	particle	926:933	arg1	PS					941:942	PS	941:942	PS	941:942	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	8	59	theme	gel	1187:1189	arg1	tendency					1094:1101	Gelation tendency	1085:1101	Gelation tendency	1085:1101	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	8	59	theme	gel	1187:1189	arg1	mucoadhesivity					1138:1151	mucoadhesivity	1138:1151	mucoadhesivity	1138:1151	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	8	59	theme	gel	1187:1189	arg1	pH					1104:1105	pH	1104:1105	pH	1104:1105	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	8	59	theme	gel	1187:1189	arg1	properties					1123:1132	shear-thinning properties	1108:1132	shear-thinning properties	1108:1132	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	0	60	theme	buccal	133:138	arg1	applicability					140:152	buccal applicability	133:152	buccal applicability	133:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	9	61	theme	AC	1236:1237	arg1	features					1247:1254	Sustained AC release features	1226:1254	Sustained AC release features	1226:1254	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	3	62	theme	optimum	583:589	arg1	MPs					591:593	the optimum MPs	579:593	the optimum MPs	579:593	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	6	63	theme	EE	989:990	arg1	efficiency					977:986	high entrapment efficiency	961:986	high entrapment efficiency (EE%) of 94.73%	961:1002	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	63	theme	EE	989:990	arg1	%					991:991	EE%	989:991	EE%	989:991	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	1	64	dep	in	239:240	arg1	situ					242:245	situ	242:245	situ	242:245	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	65	theme	therapy	394:400	arg1	limitations					352:362	limitations	352:362	limitations of oral existing conventional therapy	352:400	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	3	66	theme	gellan	622:627	arg1	gel					633:635	in situ ion-activated gellan gum gel	600:635	in situ ion-activated gellan gum gel	600:635	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
31437077	1	67	theme	buccal	212:217	arg1	applicability					219:231	the buccal applicability	208:231	the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	208:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	7	68	theme	gel	1048:1050	arg1	network					1052:1058	the gel network	1044:1058	the gel network of the composite system	1044:1082	MPs were successfully entrapped inside the gel network of the composite system.
31437077	9	69	theme	promising	1260:1268	arg1	response					1294:1301	promising in vitro anti-arthritic response	1260:1301	promising in vitro anti-arthritic response	1260:1301	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	6	70	theme	high	961:964	arg1	efficiency					977:986	high entrapment efficiency	961:986	high entrapment efficiency (EE%) of 94.73%	961:1002	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	6	70	theme	high	961:964	arg1	%					991:991	EE%	989:991	EE%	989:991	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	7	71	theme	composite	1067:1075	arg1	system					1077:1082	the composite system	1063:1082	the composite system	1063:1082	MPs were successfully entrapped inside the gel network of the composite system.
31437077	4	72	theme	state	728:732	arg1	characterizations					734:750	morphological and solid state characterizations	704:750	morphological and solid state characterizations	704:750	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	11	73	theme	composite	1493:1501	arg1	gel					1503:1505	in situ composite gel	1485:1505	in situ composite gel	1485:1505	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	5	74	theme	in	789:790	arg1	efficacy					809:816	in vivo therapeutic efficacy	789:816	in vivo therapeutic efficacy	789:816	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	11	75	dep	in	1485:1486	arg1	situ					1488:1491	situ	1488:1491	situ	1488:1491	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	5	76	theme	therapeutic	797:807	arg1	efficacy					809:816	in vivo therapeutic efficacy	789:816	in vivo therapeutic efficacy	789:816	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	11	77	theme	appropriate	1513:1523	arg1	proposal					1537:1544	an appropriate therapeutic proposal	1510:1544	an appropriate therapeutic proposal for AC buccal delivery	1510:1567	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	9	78	theme	release	1239:1245	arg1	features					1247:1254	Sustained AC release features	1226:1254	Sustained AC release features	1226:1254	Sustained AC release features and promising in vitro anti-arthritic response were also demonstrated.
31437077	5	79	theme	rats	843:846	arg1	model					834:838	inflammation model	821:838	inflammation model of rats	821:846	Suitability for buccal delivery and in vivo therapeutic efficacy in inflammation model of rats were also assessed.
31437077	1	80	theme	existing	372:379	arg1	therapy					394:400	oral existing conventional therapy	367:400	oral existing conventional therapy	367:400	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	0	81	dep	microparticles	90:103	arg1	modality					120:127	a therapeutic modality	106:127	amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability	61:152	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	11	82	theme	in	1485:1486	arg1	gel					1503:1505	in situ composite gel	1485:1505	in situ composite gel	1485:1505	Taken together; this study proves the potential of in situ composite gel as an appropriate therapeutic proposal for AC buccal delivery.
31437077	0	83	theme	ion-triggered	18:30	arg1	gel					43:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	8	84	theme	Gelation	1085:1092	arg1	tendency					1094:1101	Gelation tendency	1085:1101	Gelation tendency	1085:1101	Gelation tendency, pH, shear-thinning properties and mucoadhesivity of the prepared in situ composite gel guaranteed its buccal suitability.
31437077	0	85	theme	gum	39:41	arg1	gel					43:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel	0:45	In situ composite ion-triggered gellan gum gel incorporating amino methacrylate copolymer microparticles: a therapeutic modality for buccal applicability.
31437077	1	86	theme	amino	289:293	arg1	MPs					334:336	MPs	334:336	MPs	334:336	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	86	theme	amino	289:293	arg1	microparticles					318:331	aceclofenac (AC) amino methacrylate copolymer microparticles	272:331	aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	272:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	6	87	theme	performing	897:906	arg1	formula					908:914	the best performing formula	888:914	the best performing formula	888:914	Results showed that the best performing formula displayed particle size (PS) of 51.00 µm and high entrapment efficiency (EE%) of 94.73%.
31437077	4	88	dep	in	671:672	arg1	situ					674:677	situ	674:677	situ	674:677	The crosslinking and gelation of in situ gel were investigated by morphological and solid state characterizations.
31437077	2	89	theme	factorial	480:488	arg1	design					490:495	2241 factorial design	475:495	2241 factorial design	475:495	AC Eudragit RL100 MPs were fabricated and statistically optimized using 2241 factorial design.
31437077	10	90	theme	anti-inflammatory	1370:1386	arg1	effect					1388:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	2	91	theme	Eudragit	406:413	arg1	MPs					421:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs	403:423	AC Eudragit RL100 MPs were fabricated and statistically optimized using 2241 factorial design.
31437077	1	92	theme	copolymer	308:316	arg1	MPs					334:336	MPs	334:336	MPs	334:336	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	1	92	theme	copolymer	308:316	arg1	microparticles					318:331	aceclofenac (AC) amino methacrylate copolymer microparticles	272:331	aceclofenac (AC) amino methacrylate copolymer microparticles (MPs)	272:337	The aim of the current investigation is to delineate the buccal applicability of an in situ composite gel containing aceclofenac (AC) amino methacrylate copolymer microparticles (MPs), surmounting limitations of oral existing conventional therapy.
31437077	10	93	theme	in	1362:1363	arg1	effect					1388:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	consistent and prolonged in vivo anti-inflammatory effect	1337:1393	Moreover, consistent and prolonged in vivo anti-inflammatory effect was achieved, relative to standard AC.
31437077	3	94	theme	Better	498:503	arg1	applicability					512:524	Better buccal applicability	498:524	Better buccal applicability	498:524	Better buccal applicability and enhanced localization were achieved by combining the optimum MPs with in situ ion-activated gellan gum gel.
29508159	8	0	theme	eyelid	1099:1104	arg1	blinking					1083:1090	blinking	1083:1090	blinking of the eyelid	1083:1104	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	0	1	theme	drug	79:82	arg1	delivery					84:91	ocular drug delivery	72:91	ocular drug delivery	72:91	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	9	2	from	experiments	1278:1288	arg1	efficacy					1237:1244	the efficacy	1233:1244	the efficacy of our formulation in whole-eye experiments	1233:1288	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	9	3	with	loading	1293:1299	arg1	CBGA					1345:1348	CBGA	1345:1348	CBGA	1345:1348	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	9	3	with	loading	1293:1299	arg1	acid					1339:1342	cannabigerolic acid	1324:1342	cannabigerolic acid (CBGA)	1324:1349	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	4	4	theme	bioavailable	447:458	arg1	drugs					460:464	poorly bioavailable drugs	440:464	poorly bioavailable drugs	440:464	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	5	5	theme	methylcellulose	571:585	arg1	composite					533:541	a composite	531:541	a composite of hyaluronic acid (HA) and methylcellulose (MC)	531:590	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	5	theme	methylcellulose	571:585	arg1	hydrogel					505:512	The hydrogel	501:512	The hydrogel	501:512	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	5	theme	methylcellulose	571:585	arg1	acid					557:560	hyaluronic acid	546:560	hyaluronic acid (HA)	546:565	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	5	theme	methylcellulose	571:585	arg1	methylcellulose					571:585	methylcellulose	571:585	methylcellulose (MC)	571:590	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	4	6	dep	situ-forming	372:383	arg1	in					369:370	in	369:370	in	369:370	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	4	7	theme	aqueous	475:481	arg1	humor					483:487	the aqueous humor	471:487	the aqueous humor of the eye	471:498	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	9	8	theme	formulation	1253:1263	arg1	efficacy					1237:1244	the efficacy	1233:1244	the efficacy of our formulation in whole-eye experiments	1233:1288	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	3	9	theme	clear	250:254	arg1	need					263:266	a clear, unmet need	248:266	need	263:266	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	10	10	theme	transcorneal	1401:1412	arg1	penetration					1414:1424	transcorneal penetration	1401:1424	transcorneal penetration over control formulations	1401:1450	Our formulation exhibits over a 300% increase in transcorneal penetration over control formulations.
29508159	4	11	theme	stimulus-responsive	348:366	arg1	hydrogel					405:412	a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel	346:412	a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye	346:498	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	7	12	theme	content	781:787	arg1	identification					725:738	the identification	721:738	the identification of hydrogel composition and nanoparticle content in the formulation	721:806	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	0	13	theme	stimulus-responsive	2:20	arg1	hydrogel					59:66	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel	0:66	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.	0:92	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	9	14	theme	whole-eye	1268:1276	arg1	experiments					1278:1288	whole-eye experiments	1268:1288	whole-eye experiments	1268:1288	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	7	15	from	identification	725:738	arg1	formulation					796:806	the formulation	792:806	the formulation	792:806	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	11	16	theme	ocular	1526:1531	arg1	diseases					1533:1540	ocular diseases	1526:1540	ocular diseases	1526:1540	This work paves the way for the introduction of novel products targeting ocular diseases to the market.
29508159	11	17	theme	novel	1501:1505	arg1	products					1507:1514	novel products	1501:1514	novel products targeting ocular diseases to the market	1501:1554	This work paves the way for the introduction of novel products targeting ocular diseases to the market.
29508159	1	18	theme	Most	94:97	arg1	drops					164:168	eye drops	160:168	eye drops	160:168	Most medications targeting optic neuropathies are administered as eye drops.
29508159	1	18	theme	Most	94:97	arg1	medications					99:109	Most medications	94:109	Most medications targeting optic neuropathies	94:138	Most medications targeting optic neuropathies are administered as eye drops.
29508159	10	19	theme	%	1387:1387	arg1	increase					1389:1396	a 300% increase	1382:1396	a 300% increase in transcorneal penetration over control formulations	1382:1450	Our formulation exhibits over a 300% increase in transcorneal penetration over control formulations.
29508159	7	20	theme	temperature-dependent	870:890	arg1	rheopexy					892:899	temperature-dependent rheopexy	870:899	temperature-dependent rheopexy	870:899	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	6	21	theme	ethylene	644:651	arg1	poly					639:642	poly	639:642	poly(ethylene oxide) (PEO)	639:664	The amphiphilic nanoparticles are composed of poly(ethylene oxide) (PEO) and poly(lactic acid) (PLA).
29508159	6	21	theme	ethylene	644:651	arg1	oxide					653:657	ethylene oxide	644:657	ethylene oxide	644:657	The amphiphilic nanoparticles are composed of poly(ethylene oxide) (PEO) and poly(lactic acid) (PLA).
29508159	9	22	from	formulation	1253:1263	arg1	experiments					1278:1288	whole-eye experiments	1268:1288	whole-eye experiments	1268:1288	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	10	23	from	increase	1389:1396	arg1	penetration					1414:1424	transcorneal penetration	1401:1424	transcorneal penetration over control formulations	1401:1450	Our formulation exhibits over a 300% increase in transcorneal penetration over control formulations.
29508159	3	24	theme	novel	272:276	arg1	vehicles					305:312	novel transcorneal drug delivery vehicles	272:312	novel transcorneal drug delivery vehicles	272:312	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	2	25	theme	corneal	186:192	arg1	efficiencies					206:217	their corneal penetration efficiencies	180:217	their corneal penetration efficiencies	180:217	However, their corneal penetration efficiencies are typically < 5%.
29508159	7	26	theme	hydrogel	743:750	arg1	composition					752:762	hydrogel composition	743:762	hydrogel composition	743:762	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	9	27	from	efficacy	1237:1244	arg1	experiments					1278:1288	whole-eye experiments	1268:1288	whole-eye experiments	1268:1288	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	3	28	theme	unmet	257:261	arg1	need					263:266	a clear, unmet need	248:266	need	263:266	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	7	29	theme	composition	752:762	arg1	identification					725:738	the identification	721:738	the identification of hydrogel composition and nanoparticle content in the formulation	721:806	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	8	30	theme	eye	1157:1159	arg1	drop					1161:1164	an eye drop	1154:1164	an eye drop immediately prior to the patient's bedtime	1154:1207	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	8	30	theme	eye	1157:1159	arg1	application					1121:1131	application	1121:1131	application of the medication	1121:1149	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	7	31	theme	Experimental	695:706	arg1	design					708:713	Experimental design	695:713	Experimental design	695:713	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	10	32	theme	control	1431:1437	arg1	formulations					1439:1450	control formulations	1431:1450	control formulations	1431:1450	Our formulation exhibits over a 300% increase in transcorneal penetration over control formulations.
29508159	8	33	theme	medication	1140:1149	arg1	drop					1161:1164	an eye drop	1154:1164	an eye drop immediately prior to the patient's bedtime	1154:1207	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	8	33	theme	medication	1140:1149	arg1	application					1121:1131	application	1121:1131	application of the medication	1121:1149	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	8	34	theme	prior	1178:1182	arg1	drop					1161:1164	an eye drop	1154:1164	an eye drop immediately prior to the patient's bedtime	1154:1207	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	8	34	theme	prior	1178:1182	arg1	application					1121:1131	application	1121:1131	application of the medication	1121:1149	These properties should ensure that the formulation coats the cornea through blinking of the eyelid and facilitate application of the medication as an eye drop immediately prior to the patient's bedtime.
29508159	3	35	theme	delivery	296:303	arg1	vehicles					305:312	novel transcorneal drug delivery vehicles	272:312	novel transcorneal drug delivery vehicles	272:312	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	4	36	theme	drugs	460:464	arg1	release					429:435	controlled release	418:435	controlled release of poorly bioavailable drugs into the aqueous humor of the eye	418:498	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	7	37	theme	ocular	970:975	arg1	surface					977:983	the ocular surface	966:983	the ocular surface	966:983	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	1	38	theme	optic	121:125	arg1	neuropathies					127:138	optic neuropathies	121:138	optic neuropathies	121:138	Most medications targeting optic neuropathies are administered as eye drops.
29508159	9	39	theme	cannabigerolic	1324:1337	arg1	CBGA					1345:1348	CBGA	1345:1348	CBGA	1345:1348	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	9	39	theme	cannabigerolic	1324:1337	arg1	acid					1339:1342	cannabigerolic acid	1324:1342	cannabigerolic acid (CBGA)	1324:1349	We subsequently tested the efficacy of our formulation in whole-eye experiments by loading the nanoparticles with cannabigerolic acid (CBGA).
29508159	4	40	theme	controlled	418:427	arg1	release					429:435	controlled release	418:435	controlled release of poorly bioavailable drugs into the aqueous humor of the eye	418:498	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	0	41	dep	situ-forming	26:37	arg1	in					23:24	in	23:24	in	23:24	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	11	42	theme	products	1507:1514	arg1	introduction					1485:1496	the introduction	1481:1496	the introduction of novel products targeting ocular diseases to the market	1481:1554	This work paves the way for the introduction of novel products targeting ocular diseases to the market.
29508159	3	43	theme	transcorneal	278:289	arg1	vehicles					305:312	novel transcorneal drug delivery vehicles	272:312	novel transcorneal drug delivery vehicles	272:312	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	5	44	theme	acid	557:560	arg1	composite					533:541	a composite	531:541	a composite of hyaluronic acid (HA) and methylcellulose (MC)	531:590	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	44	theme	acid	557:560	arg1	hydrogel					505:512	The hydrogel	501:512	The hydrogel	501:512	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	44	theme	acid	557:560	arg1	acid					557:560	hyaluronic acid	546:560	hyaluronic acid (HA)	546:565	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	44	theme	acid	557:560	arg1	methylcellulose					571:585	methylcellulose	571:585	methylcellulose (MC)	571:590	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	45	theme	hyaluronic	546:555	arg1	HA					563:564	HA	563:564	HA	563:564	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	5	45	theme	hyaluronic	546:555	arg1	acid					557:560	hyaluronic acid	546:560	hyaluronic acid (HA)	546:565	The hydrogel is formulated as a composite of hyaluronic acid (HA) and methylcellulose (MC).
29508159	3	46	theme	drug	291:294	arg1	vehicles					305:312	novel transcorneal drug delivery vehicles	272:312	novel transcorneal drug delivery vehicles	272:312	There is a clear, unmet need for novel transcorneal drug delivery vehicles.
29508159	6	47	theme	lactic	675:680	arg1	poly					670:673	poly	670:673	poly(lactic acid) (PLA)	670:692	The amphiphilic nanoparticles are composed of poly(ethylene oxide) (PEO) and poly(lactic acid) (PLA).
29508159	6	47	theme	lactic	675:680	arg1	acid					682:685	lactic acid	675:685	lactic acid	675:685	The amphiphilic nanoparticles are composed of poly(ethylene oxide) (PEO) and poly(lactic acid) (PLA).
29508159	7	48	theme	nanoparticle	768:779	arg1	content					781:787	nanoparticle content	768:787	nanoparticle content	768:787	Experimental design aided the identification of hydrogel composition and nanoparticle content in the formulation, and the formulation reliably switched between thixotropy and temperature-dependent rheopexy when it was tested in a rheometer under conditions that simulate the ocular surface, including blinking.
29508159	4	49	dep	stimulus-responsive	348:366	arg1	nanoparticle-laden					386:403	nanoparticle-laden	386:403	nanoparticle-laden	386:403	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	4	49	dep	stimulus-responsive	348:366	arg1	situ-forming					372:383	situ-forming	372:383	situ-forming	372:383	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	0	50	theme	ocular	72:77	arg1	delivery					84:91	ocular drug delivery	72:91	ocular drug delivery	72:91	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	2	51	theme	penetration	194:204	arg1	efficiencies					206:217	their corneal penetration efficiencies	180:217	their corneal penetration efficiencies	180:217	However, their corneal penetration efficiencies are typically < 5%.
29508159	0	52	dep	stimulus-responsive	2:20	arg1	nanoparticle-laden					40:57	nanoparticle-laden	40:57	nanoparticle-laden	40:57	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	0	52	dep	stimulus-responsive	2:20	arg1	situ-forming					26:37	situ-forming	26:37	situ-forming	26:37	A stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for ocular drug delivery.
29508159	4	53	theme	eye	496:498	arg1	humor					483:487	the aqueous humor	471:487	the aqueous humor of the eye	471:498	To this end, we have developed a stimulus-responsive, in situ-forming, nanoparticle-laden hydrogel for controlled release of poorly bioavailable drugs into the aqueous humor of the eye.
29508159	1	54	theme	eye	160:162	arg1	medications					99:109	Most medications	94:109	Most medications targeting optic neuropathies	94:138	Most medications targeting optic neuropathies are administered as eye drops.
29508159	1	54	theme	eye	160:162	arg1	drops					164:168	eye drops	160:168	eye drops	160:168	Most medications targeting optic neuropathies are administered as eye drops.
29508159	6	55	theme	amphiphilic	597:607	arg1	nanoparticles					609:621	The amphiphilic nanoparticles	593:621	The amphiphilic nanoparticles	593:621	The amphiphilic nanoparticles are composed of poly(ethylene oxide) (PEO) and poly(lactic acid) (PLA).
30684268	19	0	from	absorption	2268:2277	arg1	tract					2299:2303	gastrointestinal tract	2282:2303	gastrointestinal tract	2282:2303	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	3	1	with	chitin	462:467	arg1	lowest					478:483	lowest	478:483	lowest	478:483	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	4	2	theme	traditional	624:634	arg1	method					636:641	traditional method	624:641	traditional method (5 hr)	624:648	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	4	2	theme	traditional	624:634	arg1	hr					646:647	5 hr	644:647	5 hr	644:647	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	2	3	theme	central	277:283	arg1	design					295:300	central composite design	277:300	central composite design (CCD) of response surface methodology (RSM)	277:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	2	3	theme	central	277:283	arg1	CCD					303:305	CCD	303:305	CCD	303:305	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	18	4	theme	squid	2145:2149	arg1	pen					2151:2153	squid pen	2145:2153	squid pen	2145:2153	Chitin from squid pen could be further converted to chitosan with high DDA.
30684268	15	5	theme	various	1865:1871	arg1	biomolecules					1873:1884	various biomolecules	1865:1884	various biomolecules	1865:1884	Ultrasonication has been used for extraction of various biomolecules from different sources.
30684268	19	6	theme	dietary	2239:2245	arg1	Chitosan					2209:2216	Chitosan	2209:2216	Chitosan	2209:2216	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	19	6	theme	dietary	2239:2245	arg1	fiber					2247:2251	a dietary fiber	2237:2251	a dietary fiber	2237:2251	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	19	6	theme	dietary	2239:2245	arg1	absorption					2268:2277	fat absorption	2264:2277	fat absorption in gastrointestinal tract	2264:2303	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	4	7	from	pens	604:607	arg1	production					582:591	chitin production	575:591	chitin production from squid pens	575:607	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	1	8	theme	squid	126:130	arg1	pens					151:154	squid (Loligo formosana) pens	126:154	squid (Loligo formosana) pens	126:154	Chitosan from squid (Loligo formosana) pens were prepared and characterized.
30684268	11	9	theme	simulated	1513:1521	arg1	conditions					1540:1549	the simulated gastrointestinal conditions	1509:1549	the simulated gastrointestinal conditions	1509:1549	CH130-2 stabilized the emulsion under the simulated gastrointestinal conditions.
30684268	5	10	theme	deacetylation	765:777	arg1	yield					745:749	yield	745:749	yield	745:749	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	5	10	theme	deacetylation	765:777	arg1	degree					755:760	degree	755:760	degree	755:760	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	5	11	theme	chitosan	788:795	arg1	DDA					780:782	DDA	780:782	DDA	780:782	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	5	11	theme	chitosan	788:795	arg1	deacetylation					765:777	deacetylation	765:777	deacetylation (DDA) of chitosan	765:795	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	12	12	theme	human	1641:1645	arg1	tract					1657:1661	the human digestive tract	1637:1661	the human digestive tract	1637:1661	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	5	13	dep	%	848:848	arg1	to					843:844	to	843:844	to	843:844	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	19	14	theme	fat	2264:2266	arg1	Chitosan					2209:2216	Chitosan	2209:2216	Chitosan	2209:2216	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	19	14	theme	fat	2264:2266	arg1	fiber					2247:2251	a dietary fiber	2237:2251	a dietary fiber	2237:2251	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	19	14	theme	fat	2264:2266	arg1	absorption					2268:2277	fat absorption	2264:2277	fat absorption in gastrointestinal tract	2264:2303	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	5	15	theme	%	827:827	arg1	range					809:813	the range	805:813	the range of 50% to 65% (w/w) and 78% to 90%	805:848	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	2	16	theme	pens	266:269	arg1	deproteinization					240:255	deproteinization	240:255	deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM)	240:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	1	17	theme	Loligo	133:138	arg1	pens					151:154	squid (Loligo formosana) pens	126:154	squid (Loligo formosana) pens	126:154	Chitosan from squid (Loligo formosana) pens were prepared and characterized.
30684268	12	18	from	adsorption	1612:1621	arg1	tract					1657:1661	the human digestive tract	1637:1661	the human digestive tract	1637:1661	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	5	19	theme	different	711:719	arg1	temperatures					721:732	different temperatures	711:732	different temperatures	711:732	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	1	20	from	pens	151:154	arg1	Chitosan					112:119	Chitosan	112:119	Chitosan from squid (Loligo formosana) pens	112:154	Chitosan from squid (Loligo formosana) pens were prepared and characterized.
30684268	3	21	theme	Squid	347:351	arg1	pens					353:356	Squid pens	347:356	Squid pens	347:356	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	15	22	from	sources	1901:1907	arg1	extraction					1851:1860	extraction	1851:1860	extraction of various biomolecules from different sources	1851:1907	Ultrasonication has been used for extraction of various biomolecules from different sources.
30684268	0	23	theme	Binding	94:100	arg1	Capacity					102:109	Fat Binding Capacity	90:109	Fat Binding Capacity	90:109	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	6	24	theme	Intrinsic	851:859	arg1	viscosity					861:869	Intrinsic viscosity	851:869	Intrinsic viscosity	851:869	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	0	25	from	Pen	54:56	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Chitosan from Squid Pen	0:56	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	13	26	theme	crustacean	1749:1758	arg1	shells					1760:1765	crustacean shells	1749:1765	crustacean shells	1749:1765	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	20	27	theme	squid	2384:2388	arg1	pen					2390:2392	squid pen	2384:2392	squid pen	2384:2392	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	20	27	theme	squid	2384:2388	arg1	byproduct					2408:2416	a processing byproduct	2395:2416	a processing byproduct	2395:2416	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	9	28	theme	CH130-2	1255:1261	arg1	DDA					1248:1250	DDA	1248:1250	DDA of CH130-2 determined using 1 H-NMR	1248:1286	DDA of CH130-2 determined using 1 H-NMR was 89%, which was similar to that (87%) obtained from FTIR.
30684268	14	29	theme	chitin	1791:1796	arg1	extraction					1777:1786	extraction	1777:1786	extraction of chitin	1777:1796	However, extraction of chitin is time consuming.
30684268	0	30	theme	Ultrasound-Assisted	0:18	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Chitosan from Squid Pen	0:56	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	17	31	with	ultrasonication	2009:2023	arg1	condition					2040:2048	optimized condition	2030:2048	optimized condition using RSM	2030:2058	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	3	32	theme	1:18	439:442	arg1	ratio					430:434	the solid/solvent ratio	412:434	the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein	412:501	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	10	33	theme	%	1441:1441	arg1	%					1448:1448	55% to 27%	1439:1448	55% to 27% after deacetylation	1439:1468	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	5	34	theme	%	841:841	arg1	%					848:848	78% to 90%	839:848	78% to 90%	839:848	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	13	35	dep	APPLICATION	1674:1684	arg1	Chitin					1687:1692	Chitin	1687:1692	Chitin	1687:1692	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	13	35	dep	APPLICATION	1674:1684	arg1	chitosan					1698:1705	chitosan	1698:1705	chitosan	1698:1705	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	12	36	used	used	1575:1578	arg2	it					1563:1564	it	1563:1564	it	1563:1564	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	12	36	used	used	1575:1578	arg2	fiber					1591:1595	dietary fiber	1583:1595	dietary fiber	1583:1595	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	6	37	dep	6.52	937:940	arg1	to					934:935	to	934:935	to	934:935	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	38	dep	×	968:968	arg1	to					961:962	to	961:962	to	961:962	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	7	39	theme	gastrointestinal	1095:1110	arg1	tract					1112:1116	gastrointestinal tract	1095:1116	gastrointestinal tract	1095:1116	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	0	40	theme	Molecular	59:67	arg1	Characterization					69:84	Molecular Characterization	59:84	Molecular Characterization	59:84	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	8	41	theme	optimum	1194:1200	arg1	yield					1202:1206	the optimum yield	1190:1206	the optimum yield (54%)	1190:1212	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	8	41	theme	optimum	1194:1200	arg1	%					1211:1211	54%	1209:1211	54%	1209:1211	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	6	42	theme	105	957:959	arg1	×					968:968	×	968:968	×	968:968	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	2	43	theme	ultrasonication	196:210	arg1	condition					212:220	ultrasonication condition	196:220	ultrasonication condition	196:220	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	6	44	dep	×	955:955	arg1	Da					974:975	105 to 3.2 × 105 Da	957:975	1.2 × 105 to 3.2 × 105 Da	951:975	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	2	45	theme	methodology	328:338	arg1	design					295:300	central composite design	277:300	central composite design (CCD) of response surface methodology (RSM)	277:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	2	45	theme	methodology	328:338	arg1	CCD					303:305	CCD	303:305	CCD	303:305	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	7	46	theme	mimicked	1070:1077	arg1	condition					1082:1090	the mimicked pH condition	1066:1090	the mimicked pH condition of gastrointestinal tract	1066:1116	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	6	47	theme	dL/g	942:945	arg1	range					921:925	the range	917:925	the range of 3.2 to 6.52 dL/g	917:945	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	47	theme	dL/g	942:945	arg1	×					955:955	1.2 ×	951:955	1.2 × 105 to 3.2 × 105 Da	951:975	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	8	48	contain	had	1218:1220	arg2	Da					1243:1244	1.5 × 105 Da	1233:1244	1.5 × 105 Da	1233:1244	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	8	48	contain	had	1218:1220	arg2	MW					1229:1230	medium MW	1222:1230	medium MW (1.5 × 105 Da)	1222:1245	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	8	48	contain	had	1218:1220	arg1	Chitosan					1119:1126	Chitosan	1119:1126	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2)	1119:1181	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	10	49	theme	crystallinity	1414:1426	arg1	index					1428:1432	crystallinity index	1414:1432	crystallinity index	1414:1432	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	7	50	theme	oil	1047:1049	arg1	droplets					1051:1058	oil droplets	1047:1058	oil droplets	1047:1058	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	17	51	theme	ultrasonication	2009:2023	arg1	application					1994:2004	application	1994:2004	application of ultrasonication with optimized condition using RSM	1994:2058	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	15	52	used	used	1842:1845	arg2	Ultrasonication					1817:1831	Ultrasonication	1817:1831	Ultrasonication	1817:1831	Ultrasonication has been used for extraction of various biomolecules from different sources.
30684268	15	53	theme	different	1891:1899	arg1	sources					1901:1907	different sources	1891:1907	different sources	1891:1907	Ultrasonication has been used for extraction of various biomolecules from different sources.
30684268	4	54	theme	extraction	555:564	arg1	time					566:569	the extraction time	551:569	the extraction time for chitin production from squid pens	551:607	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	16	55	theme	processing	1936:1945	arg1	time					1947:1950	the processing time	1932:1950	the processing time	1932:1950	It effectively lowers the processing time and enhances extraction yield.
30684268	13	56	theme	PRACTICAL	1664:1672	arg1	APPLICATION					1674:1684	PRACTICAL APPLICATION	1664:1684	PRACTICAL APPLICATION: Chitin and chitosan	1664:1705	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	13	56	theme	PRACTICAL	1664:1672	arg1	products					1722:1729	marketable products	1711:1729	marketable products manufactured from crustacean shells	1711:1765	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	2	57	theme	composite	285:293	arg1	design					295:300	central composite design	277:300	central composite design (CCD) of response surface methodology (RSM)	277:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	2	57	theme	composite	285:293	arg1	CCD					303:305	CCD	303:305	CCD	303:305	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	15	58	theme	biomolecules	1873:1884	arg1	extraction					1851:1860	extraction	1851:1860	extraction of various biomolecules from different sources	1851:1907	Ultrasonication has been used for extraction of various biomolecules from different sources.
30684268	2	59	theme	response	311:318	arg1	RSM					341:343	RSM	341:343	RSM	341:343	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	2	59	theme	response	311:318	arg1	methodology					328:338	response surface methodology	311:338	response surface methodology (RSM)	311:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	12	60	theme	digestive	1647:1655	arg1	tract					1657:1661	the human digestive tract	1637:1661	the human digestive tract	1637:1661	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	6	61	theme	105	970:972	arg1	×					968:968	×	968:968	×	968:968	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	62	theme	3.2	964:966	arg1	×					968:968	×	968:968	×	968:968	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	4	63	theme	squid	598:602	arg1	pens					604:607	squid pens	598:607	squid pens	598:607	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	5	64	theme	resultant	660:668	arg1	chitin					670:675	the resultant chitin	656:675	the resultant chitin	656:675	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	12	65	theme	fat/oil	1626:1632	arg1	adsorption					1612:1621	the adsorption	1608:1621	the adsorption of fat/oil in the human digestive tract	1608:1661	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	8	66	theme	medium	1222:1227	arg1	Da					1243:1244	1.5 × 105 Da	1233:1244	1.5 × 105 Da	1233:1244	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	8	66	theme	medium	1222:1227	arg1	MW					1229:1230	medium MW	1222:1230	medium MW (1.5 × 105 Da)	1222:1245	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	7	67	with	chitosans	1000:1008	arg1	DDA					1025:1027	different DDA	1015:1027	different DDA	1015:1027	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	11	68	theme	gastrointestinal	1523:1538	arg1	conditions					1540:1549	the simulated gastrointestinal conditions	1509:1549	the simulated gastrointestinal conditions	1509:1549	CH130-2 stabilized the emulsion under the simulated gastrointestinal conditions.
30684268	17	69	theme	optimized	2030:2038	arg1	condition					2040:2048	optimized condition	2030:2048	optimized condition using RSM	2030:2058	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	10	70	dep	%	1448:1448	arg1	to					1443:1444	to	1443:1444	to	1443:1444	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	8	71	theme	×	1237:1237	arg1	Da					1243:1244	1.5 × 105 Da	1233:1244	1.5 × 105 Da	1233:1244	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	8	71	theme	×	1237:1237	arg1	MW					1229:1230	medium MW	1222:1230	medium MW (1.5 × 105 Da)	1222:1245	Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da).
30684268	10	72	theme	chitin	1383:1388	arg1	structure					1390:1398	chitin structure	1383:1398	chitin structure	1383:1398	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	20	73	theme	processing	2353:2362	arg1	industry					2364:2371	squid processing industry	2347:2371	squid processing industry	2347:2371	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	2	74	theme	squid	260:264	arg1	pens					266:269	squid pens	260:269	squid pens using central composite design (CCD) of response surface methodology (RSM)	260:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	1	75	theme	formosana	140:148	arg1	pens					151:154	squid (Loligo formosana) pens	126:154	squid (Loligo formosana) pens	126:154	Chitosan from squid (Loligo formosana) pens were prepared and characterized.
30684268	7	76	theme	different	1015:1023	arg1	DDA					1025:1027	different DDA	1015:1027	different DDA	1015:1027	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	0	77	theme	Fat	90:92	arg1	Capacity					102:109	Fat Binding Capacity	90:109	Fat Binding Capacity	90:109	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	5	78	theme	%	820:820	arg1	%					827:827	50% to 65%	818:827	50% to 65% (w/w)	818:833	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	5	78	theme	%	820:820	arg1	w/w					830:832	w/w	830:832	w/w	830:832	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	6	79	theme	molecular	875:883	arg1	MW					893:894	MW	893:894	MW	893:894	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	79	theme	molecular	875:883	arg1	weight					885:890	molecular weight	875:890	molecular weight (MW)	875:895	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	19	80	theme	gastrointestinal	2282:2297	arg1	tract					2299:2303	gastrointestinal tract	2282:2303	gastrointestinal tract	2282:2303	Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
30684268	18	81	theme	high	2199:2202	arg1	DDA					2204:2206	high DDA	2199:2206	high DDA	2199:2206	Chitin from squid pen could be further converted to chitosan with high DDA.
30684268	12	82	theme	dietary	1583:1589	arg1	fiber					1591:1595	dietary fiber	1583:1595	dietary fiber	1583:1595	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	12	82	theme	dietary	1583:1589	arg1	it					1563:1564	it	1563:1564	it	1563:1564	Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.
30684268	5	83	theme	%	848:848	arg1	range					809:813	the range	805:813	the range of 50% to 65% (w/w) and 78% to 90%	805:848	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	17	84	from	pen	2128:2130	arg1	yield					2101:2105	yield	2101:2105	yield of chitin from squid pen	2101:2130	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	5	85	dep	%	827:827	arg1	to					822:823	to	822:823	to	822:823	When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%.
30684268	16	86	theme	extraction	1965:1974	arg1	yield					1976:1980	extraction yield	1965:1980	extraction yield	1965:1980	It effectively lowers the processing time and enhances extraction yield.
30684268	3	87	theme	solid/solvent	416:428	arg1	ratio					430:434	the solid/solvent ratio	412:434	the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein	412:501	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	20	88	theme	processing	2397:2406	arg1	pen					2390:2392	squid pen	2384:2392	squid pen	2384:2392	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	20	88	theme	processing	2397:2406	arg1	byproduct					2408:2416	a processing byproduct	2395:2416	a processing byproduct	2395:2416	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	3	89	theme	amplitude	381:389	arg1	%					393:393	amplitude 69%	381:393	amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein	381:501	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	17	90	theme	chitin	2110:2115	arg1	yield					2101:2105	yield	2101:2105	yield of chitin from squid pen	2101:2130	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	0	91	theme	Chitosan	34:41	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Chitosan from Squid Pen	0:56	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	0	92	dep	Characterization	69:84	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Chitosan from Squid Pen	0:56	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	7	93	theme	tract	1112:1116	arg1	condition					1082:1090	the mimicked pH condition	1066:1090	the mimicked pH condition of gastrointestinal tract	1066:1116	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	17	94	theme	squid	2122:2126	arg1	pen					2128:2130	squid pen	2122:2130	squid pen	2122:2130	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
30684268	0	95	theme	Squid	48:52	arg1	Pen					54:56	Squid Pen	48:56	Squid Pen	48:56	Ultrasound-Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity.
30684268	6	96	theme	chitosan	900:907	arg1	MW					893:894	MW	893:894	MW	893:894	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	96	theme	chitosan	900:907	arg1	viscosity					861:869	Intrinsic viscosity	851:869	Intrinsic viscosity	851:869	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	6	96	theme	chitosan	900:907	arg1	weight					885:890	molecular weight	875:890	molecular weight (MW)	875:895	Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively.
30684268	3	97	from	ratio	430:434	arg1	min					405:407	41.46 min	399:407	41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein	399:501	Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein.
30684268	10	98	theme	XRD	1349:1351	arg1	results					1353:1359	XRD results	1349:1359	XRD results	1349:1359	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	7	99	theme	pH	1079:1080	arg1	condition					1082:1090	the mimicked pH condition	1066:1090	the mimicked pH condition of gastrointestinal tract	1066:1116	All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract.
30684268	13	100	theme	marketable	1711:1720	arg1	APPLICATION					1674:1684	PRACTICAL APPLICATION	1664:1684	PRACTICAL APPLICATION: Chitin and chitosan	1664:1705	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	13	100	theme	marketable	1711:1720	arg1	products					1722:1729	marketable products	1711:1729	marketable products manufactured from crustacean shells	1711:1765	PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells.
30684268	10	101	theme	structure	1390:1398	arg1	destruction					1368:1378	destruction	1368:1378	destruction of chitin structure	1368:1398	XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation.
30684268	20	102	theme	squid	2347:2351	arg1	industry					2364:2371	squid processing industry	2347:2371	squid processing industry	2347:2371	Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
30684268	4	103	theme	chitin	575:580	arg1	production					582:591	chitin production	575:591	chitin production from squid pens	575:607	Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr).
30684268	2	104	theme	surface	320:326	arg1	RSM					341:343	RSM	341:343	RSM	341:343	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	2	104	theme	surface	320:326	arg1	methodology					328:338	response surface methodology	311:338	response surface methodology (RSM)	311:344	First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM).
30684268	18	105	from	pen	2151:2153	arg1	Chitin					2133:2138	Chitin	2133:2138	Chitin from squid pen	2133:2153	Chitin from squid pen could be further converted to chitosan with high DDA.
30684268	17	106	theme	extraction	2073:2082	arg1	time					2084:2087	extraction time	2073:2087	extraction time	2073:2087	Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen.
31202295	0	0	theme	potato	60:65	arg1	structure					24:32	molecular structure	14:32	molecular structure of oca (Oxalis tuberosa), potato, and maize starches	14:85	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	4	1	theme	amylose	415:421	arg1	content					423:429	The amylose content	411:429	The amylose content of oca starch (∼21%)	411:450	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	1	theme	amylose	415:421	arg1	lower					456:460	lower	456:460	lower	456:460	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	8	2	theme	B-type	905:910	arg1	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	8	3	theme	starch	844:849	arg1	granules					851:858	Oca starch granules	840:858	Oca starch granules	840:858	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	9	4	contain	has	970:972	arg1	starch					963:968	oca starch	959:968	oca starch	959:968	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31202295	9	4	contain	has	970:972	arg2	potential					978:986	the potential to be developed as a novel starch source	974:1027	the potential to be developed as a novel starch source	974:1027	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31202295	4	5	theme	oca	434:436	arg1	starch					438:443	oca starch	434:443	oca starch (∼21%)	434:450	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	5	theme	oca	434:436	arg1	%					449:449	∼21%	446:449	∼21%	446:449	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	6	6	theme	shorter	687:693	arg1	length					710:715	a shorter internal chain length	685:715	a shorter internal chain length	685:715	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	5	7	theme	fine	544:547	arg1	similar					592:598	similar	592:598	similar	592:598	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	5	7	theme	fine	544:547	arg1	structure					549:557	The fine structure	540:557	The fine structure of oca amylopectin	540:576	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	2	8	theme	oca	244:246	arg1	varieties					248:256	two commercial oca varieties	229:256	two commercial oca varieties grown in New Zealand	229:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	8	9	contain	had	860:862	arg2	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	8	9	contain	had	860:862	arg1	granules					851:858	Oca starch granules	840:858	Oca starch granules	840:858	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	9	10	theme	Comparative	923:933	arg1	analysis					935:942	Comparative analysis	923:942	Comparative analysis	923:942	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31202295	8	11	theme	moment	873:878	arg1	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	2	12	theme	commercial	233:242	arg1	varieties					248:256	two commercial oca varieties	229:256	two commercial oca varieties grown in New Zealand	229:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	13	from	Composition	176:186	arg1	tubers					219:224	tubers	219:224	tubers of two commercial oca varieties grown in New Zealand	219:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	0	14	theme	starches	78:85	arg1	structure					24:32	molecular structure	14:32	molecular structure of oca (Oxalis tuberosa), potato, and maize starches	14:85	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	8	15	dep	34.5 μm	893:899	arg1	polymorph					912:920	polymorph	912:920	polymorph	912:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	4	16	theme	A	515:515	arg1	method					531:536	concanavalin A precipitation method	502:536	concanavalin A precipitation method	502:536	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	16	theme	A	515:515	arg1	maize					475:479	maize	475:479	maize	475:479	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	3	17	theme	oca	365:367	arg1	starch					369:374	oca starch	365:374	oca starch	365:374	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	1	18	theme	underutilized	116:128	arg1	species					130:136	an underutilized species	113:136	an underutilized species	113:136	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	1	18	theme	underutilized	116:128	arg1	Oca					88:90	Oca	88:90	Oca (Oxalis tuberosa)	88:108	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	0	19	theme	maize	72:76	arg1	starches					78:85	maize starches	72:85	maize starches	72:85	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	5	20	theme	maize	646:650	arg1	amylopectin					652:662	maize amylopectin	646:662	maize amylopectin	646:662	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	6	21	theme	Oca	665:667	arg1	amylopectin					669:679	Oca amylopectin	665:679	Oca amylopectin	665:679	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	2	22	from	structure	192:200	arg1	tubers					219:224	tubers	219:224	tubers of two commercial oca varieties grown in New Zealand	219:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	5	23	theme	potato	611:616	arg1	amylopectin					618:628	potato amylopectin	611:628	potato amylopectin	611:628	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	6	24	theme	potato	752:757	arg1	amylopectin					759:769	potato amylopectin	752:769	potato amylopectin	752:769	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	8	25	theme	mean	880:883	arg1	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	2	26	theme	New	267:269	arg1	Zealand					271:277	New Zealand	267:277	New Zealand	267:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	27	theme	potato	322:327	arg1	starches					329:336	normal maize and potato starches	305:336	normal maize and potato starches	305:336	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	8	28	theme	34.5 μm	893:899	arg1	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	3	29	theme	starch	369:374	arg1	that					388:391	that	388:391	that	388:391	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	29	theme	starch	369:374	arg1	content					354:360	The phosphorus content	339:360	The phosphorus content of oca starch	339:374	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	29	theme	starch	369:374	arg1	%					383:383	∼60%	380:383	∼60% of that of potato starch	380:408	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	0	30	theme	structure	24:32	arg1	Comparison					0:9	Comparison	0:9	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.	0:86	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	8	31	theme	volume	866:871	arg1	size					885:888	a volume moment mean size	864:888	a volume moment mean size of 34.5 μm and B-type polymorph	864:920	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	3	32	theme	that	388:391	arg1	content					354:360	The phosphorus content	339:360	The phosphorus content of oca starch	339:374	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	32	theme	that	388:391	arg1	that					388:391	that	388:391	that	388:391	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	32	theme	that	388:391	arg1	%					383:383	∼60%	380:383	∼60% of that of potato starch	380:408	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	0	33	theme	molecular	14:22	arg1	structure					24:32	molecular structure	14:32	molecular structure of oca (Oxalis tuberosa), potato, and maize starches	14:85	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	2	34	theme	varieties	248:256	arg1	tubers					219:224	tubers	219:224	tubers of two commercial oca varieties grown in New Zealand	219:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	35	from	tubers	219:224	arg1	starches					205:212	starches	205:212	starches from tubers of two commercial oca varieties grown in New Zealand	205:277	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	35	from	tubers	219:224	arg1	structure					192:200	structure	192:200	structure	192:200	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	35	from	tubers	219:224	arg1	Composition					176:186	Composition	176:186	Composition	176:186	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	1	36	theme	novel	155:159	arg1	source					168:173	a novel starch source	153:173	a novel starch source	153:173	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	0	37	theme	oca	37:39	arg1	structure					24:32	molecular structure	14:32	molecular structure of oca (Oxalis tuberosa), potato, and maize starches	14:85	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	1	38	theme	starch	161:166	arg1	source					168:173	a novel starch source	153:173	a novel starch source	153:173	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	6	39	theme	fingerprint	726:736	arg1	B-chains					738:745	less fingerprint B-chains	721:745	less fingerprint B-chains	721:745	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	0	40	theme	Oxalis	42:47	arg1	oca					37:39	oca	37:39	oca (Oxalis tuberosa)	37:57	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	0	40	theme	Oxalis	42:47	arg1	tuberosa					49:56	Oxalis tuberosa	42:56	Oxalis tuberosa	42:56	Comparison of molecular structure of oca (Oxalis tuberosa), potato, and maize starches.
31202295	4	41	theme	starch	438:443	arg1	content					423:429	The amylose content	411:429	The amylose content of oca starch (∼21%)	411:450	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	41	theme	starch	438:443	arg1	lower					456:460	lower	456:460	lower	456:460	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	3	42	theme	phosphorus	343:352	arg1	that					388:391	that	388:391	that	388:391	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	42	theme	phosphorus	343:352	arg1	content					354:360	The phosphorus content	339:360	The phosphorus content of oca starch	339:374	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	3	42	theme	phosphorus	343:352	arg1	%					383:383	∼60%	380:383	∼60% of that of potato starch	380:408	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	5	43	theme	oca	562:564	arg1	amylopectin					566:576	oca amylopectin	562:576	oca amylopectin	562:576	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	9	44	theme	novel	1009:1013	arg1	source					1022:1027	a novel starch source	1007:1027	a novel starch source	1007:1027	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31202295	5	45	theme	amylopectin	566:576	arg1	similar					592:598	similar	592:598	similar	592:598	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	5	45	theme	amylopectin	566:576	arg1	structure					549:557	The fine structure	540:557	The fine structure of oca amylopectin	540:576	The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin.
31202295	9	46	theme	starch	1015:1020	arg1	source					1022:1027	a novel starch source	1007:1027	a novel starch source	1007:1027	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31202295	3	47	theme	potato	396:401	arg1	starch					403:408	potato starch	396:408	potato starch	396:408	The phosphorus content of oca starch was ∼60% of that of potato starch.
31202295	6	48	theme	chain	704:708	arg1	length					710:715	a shorter internal chain length	685:715	a shorter internal chain length	685:715	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	8	49	theme	Oca	840:842	arg1	granules					851:858	Oca starch granules	840:858	Oca starch granules	840:858	Oca starch granules had a volume moment mean size of 34.5 μm and B-type polymorph.
31202295	1	50	theme	Oxalis	93:98	arg1	Oca					88:90	Oca	88:90	Oca (Oxalis tuberosa)	88:108	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	1	50	theme	Oxalis	93:98	arg1	tuberosa					100:107	Oxalis tuberosa	93:107	Oxalis tuberosa	93:107	Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source.
31202295	4	51	theme	potato	485:490	arg1	starches					492:499	potato starches	485:499	potato starches	485:499	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	6	52	theme	internal	695:702	arg1	length					710:715	a shorter internal chain length	685:715	a shorter internal chain length	685:715	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	2	53	theme	starches	205:212	arg1	structure					192:200	structure	192:200	structure	192:200	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	2	53	theme	starches	205:212	arg1	Composition					176:186	Composition	176:186	Composition	176:186	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	4	54	theme	precipitation	517:529	arg1	method					531:536	concanavalin A precipitation method	502:536	concanavalin A precipitation method	502:536	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	54	theme	precipitation	517:529	arg1	maize					475:479	maize	475:479	maize	475:479	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	2	55	theme	maize	312:316	arg1	starches					329:336	normal maize and potato starches	305:336	normal maize and potato starches	305:336	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	7	56	theme	oca	780:782	arg1	similar					831:837	similar	831:837	similar	831:837	The two oca starches were structurally and compositionally similar.
31202295	7	56	theme	oca	780:782	arg1	starches					784:791	The two oca starches	772:791	The two oca starches	772:791	The two oca starches were structurally and compositionally similar.
31202295	4	57	theme	concanavalin	502:513	arg1	method					531:536	concanavalin A precipitation method	502:536	concanavalin A precipitation method	502:536	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	4	57	theme	concanavalin	502:513	arg1	maize					475:479	maize	475:479	maize	475:479	The amylose content of oca starch (∼21%) was lower than that of maize and potato starches (concanavalin A precipitation method).
31202295	6	58	contain	had	681:683	arg2	B-chains					738:745	less fingerprint B-chains	721:745	less fingerprint B-chains	721:745	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	6	58	contain	had	681:683	arg1	amylopectin					669:679	Oca amylopectin	665:679	Oca amylopectin	665:679	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	6	58	contain	had	681:683	arg2	length					710:715	a shorter internal chain length	685:715	a shorter internal chain length	685:715	Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin.
31202295	2	59	theme	normal	305:310	arg1	starches					329:336	normal maize and potato starches	305:336	normal maize and potato starches	305:336	Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches.
31202295	9	60	theme	oca	959:961	arg1	starch					963:968	oca starch	959:968	oca starch	959:968	Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
31132052	8	0	theme	porous	1740:1745	arg1	structure					1747:1755	metallized palladium porous structure	1719:1755	metallized palladium porous structure	1719:1755	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	0	theme	porous	1740:1745	arg1	area					1713:1716	a high surface area	1698:1716	a high surface area	1698:1716	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	5	1	theme	CO2	967:969	arg1	drying					985:990	CO2 supercritical drying	967:990	CO2 supercritical drying	967:990	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	6	2	theme	other	1247:1251	arg1	techniques					1261:1270	other sol-gel techniques	1247:1270	other sol-gel techniques	1247:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	4	3	theme	ethylenediamine	712:726	arg1	presence					700:707	the presence	696:707	the presence of ethylenediamine	696:726	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	5	4	theme	ethanol	937:943	arg1	exchange					953:960	ethanol solvent exchange	937:960	ethanol solvent exchange	937:960	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	5	with	reduction	869:877	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	5	with	reduction	869:877	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	2	6	with	aerogels	251:258	arg1	control					276:282	poor shape control	265:282	poor shape control	265:282	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	8	7	theme	electron	1527:1534	arg1	microscopy					1536:1545	scanning electron microscopy	1518:1545	scanning electron microscopy	1518:1545	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	5	8	theme	high	884:887	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	9	theme	rinsing	919:925	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	6	10	theme	precursor	1005:1013	arg1	concentration					1029:1041	the precursor palladium ion concentration	1001:1041	the precursor palladium ion concentration	1001:1041	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	7	11	theme	thin	1428:1431	arg1	films					1433:1437	thin films	1428:1437	thin films	1428:1437	With diffusion as the basis to introduce and remove chemical species into and out of the hydrogel, this method is suitable for smaller bulk geometries and thin films.
31132052	5	12	theme	CNF	733:735	arg1	hydrogels					737:745	The CNF hydrogels	729:745	The CNF hydrogels	729:745	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	8	13	with	aerogels	1504:1511	arg1	microscopy					1536:1545	scanning electron microscopy	1518:1545	scanning electron microscopy	1518:1545	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	13	with	aerogels	1504:1511	arg1	voltammetry					1676:1686	cyclic voltammetry	1669:1686	cyclic voltammetry	1669:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	13	with	aerogels	1504:1511	arg1	analysis					1590:1597	thermal gravimetric analysis	1570:1597	thermal gravimetric analysis	1570:1597	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	13	with	aerogels	1504:1511	arg1	spectroscopy					1651:1662	electrochemical impedance spectroscopy	1625:1662	electrochemical impedance spectroscopy	1625:1662	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	13	with	aerogels	1504:1511	arg1	diffractometry					1554:1567	X-ray diffractometry	1548:1567	X-ray diffractometry	1548:1567	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	13	with	aerogels	1504:1511	arg1	adsorption					1613:1622	nitrogen gas adsorption	1600:1622	nitrogen gas adsorption	1600:1622	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	5	14	theme	supercritical	971:983	arg1	drying					985:990	CO2 supercritical drying	967:990	CO2 supercritical drying	967:990	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	3	15	theme	cellulose	314:322	arg1	nanofibers					324:333	carboxymethylated cellulose nanofibers	296:333	carboxymethylated cellulose nanofibers (CNFs)	296:340	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	3	15	theme	cellulose	314:322	arg1	CNFs					336:339	CNFs	336:339	CNFs	336:339	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	5	16	with	equilibration	828:840	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	16	with	equilibration	828:840	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	3	17	with	CNFs	443:446	arg1	control					453:459	control	453:459	control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying	453:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	1	18	theme	palladium	146:154	arg1	aerogels					166:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	8	19	theme	thermal	1570:1576	arg1	analysis					1590:1597	thermal gravimetric analysis	1570:1597	thermal gravimetric analysis	1570:1597	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	7	20	theme	smaller	1400:1406	arg1	geometries					1413:1422	smaller bulk geometries	1400:1422	smaller bulk geometries	1400:1422	With diffusion as the basis to introduce and remove chemical species into and out of the hydrogel, this method is suitable for smaller bulk geometries and thin films.
31132052	2	21	theme	poor	265:268	arg1	control					276:282	poor shape control	265:282	poor shape control	265:282	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	8	22	theme	nitrogen	1600:1607	arg1	adsorption					1613:1622	nitrogen gas adsorption	1600:1622	nitrogen gas adsorption	1600:1622	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	5	23	with	drying	985:990	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	23	with	drying	985:990	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	24	theme	covalent	805:812	arg1	crosslinking					814:825	covalent crosslinking	805:825	covalent crosslinking	805:825	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	0	25	theme	Biotemplated	41:52	arg1	Aerogels					74:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	6	26	theme	chemical	1137:1144	arg1	reduction					1146:1154	a direct ion chemical reduction	1124:1154	a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques	1124:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	8	27	theme	electrochemical	1625:1639	arg1	spectroscopy					1651:1662	electrochemical impedance spectroscopy	1625:1662	electrochemical impedance spectroscopy	1625:1662	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	0	28	theme	Synthesis	0:8	arg1	Method					10:15	Synthesis Method	0:15	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.	0:82	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	6	29	theme	direct	1126:1131	arg1	reduction					1146:1154	a direct ion chemical reduction	1124:1154	a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques	1124:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	4	30	theme	carboxymethylated	569:585	arg1	nanofibers					597:606	the carboxymethylated cellulose nanofibers	565:606	Crosslinking the carboxymethylated cellulose nanofibers	552:606	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	5	31	theme	concentration	889:901	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	1	32	theme	composite	156:164	arg1	aerogels					166:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	2	33	theme	metal	195:199	arg1	methods					219:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	0	34	theme	Nanofiber	31:39	arg1	Aerogels					74:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	6	35	theme	aerogel	1098:1104	arg1	composite					1106:1114	the final aerogel composite	1088:1114	the final aerogel composite	1088:1114	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	3	36	theme	metal	407:411	arg1	ions					413:416	metal ions	407:416	metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying	407:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	3	36	theme	metal	407:411	arg1	palladium					426:434	palladium	426:434	palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying	426:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	37	theme	nanofiber-palladium	1474:1492	arg1	aerogels					1504:1511	the cellulose nanofiber-palladium composite aerogels	1460:1511	the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry	1460:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	3	38	theme	macroscopic	489:499	arg1	shape					518:522	macroscopic aerogel monolith shape	489:522	macroscopic aerogel monolith shape	489:522	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	5	39	theme	metal	863:867	arg1	reduction					869:877	metal reduction	863:877	metal reduction with high concentration reducing agent, rinsing in water	863:934	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	6	40	theme	slow	1194:1197	arg1	coalescence					1199:1209	the relatively slow coalescence	1179:1209	the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques	1179:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	8	41	theme	aerogels	1504:1511	arg1	Characterization					1440:1455	Characterization	1440:1455	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry	1440:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	42	theme	surface	1705:1711	arg1	structure					1747:1755	metallized palladium porous structure	1719:1755	metallized palladium porous structure	1719:1755	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	42	theme	surface	1705:1711	arg1	area					1713:1716	a high surface area	1698:1716	a high surface area	1698:1716	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	2	43	theme	synthesis	209:217	arg1	methods					219:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	8	44	theme	scanning	1518:1525	arg1	microscopy					1536:1545	scanning electron microscopy	1518:1545	scanning electron microscopy	1518:1545	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	4	45	theme	carbodiimide	660:671	arg1	EDC					688:690	EDC	688:690	EDC	688:690	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	4	45	theme	carbodiimide	660:671	arg1	hydrochloride					673:685	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride	626:685	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)	626:691	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	3	46	theme	monolith	509:516	arg1	shape					518:522	macroscopic aerogel monolith shape	489:522	macroscopic aerogel monolith shape	489:522	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	47	theme	palladium	1730:1738	arg1	structure					1747:1755	metallized palladium porous structure	1719:1755	metallized palladium porous structure	1719:1755	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	47	theme	palladium	1730:1738	arg1	area					1713:1716	a high surface area	1698:1716	a high surface area	1698:1716	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	6	48	theme	sol-gel	1253:1259	arg1	techniques					1261:1270	other sol-gel techniques	1247:1270	other sol-gel techniques	1247:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	4	49	theme	1-ethyl-3-	626:635	arg1	carbodiimide					660:671	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	626:671	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)	626:691	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	0	50	theme	Palladium	54:62	arg1	Aerogels					74:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	6	51	theme	nanoparticles	1225:1237	arg1	coalescence					1199:1209	the relatively slow coalescence	1179:1209	the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques	1179:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	5	52	theme	reducing	903:910	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	3	53	theme	carboxymethylated	296:312	arg1	nanofibers					324:333	carboxymethylated cellulose nanofibers	296:333	carboxymethylated cellulose nanofibers (CNFs)	296:340	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	3	53	theme	carboxymethylated	296:312	arg1	CNFs					336:339	CNFs	336:339	CNFs	336:339	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	6	54	theme	palladium	1015:1023	arg1	concentration					1029:1041	the precursor palladium ion concentration	1001:1041	the precursor palladium ion concentration	1001:1041	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	8	55	theme	X-ray	1548:1552	arg1	diffractometry					1554:1567	X-ray diffractometry	1548:1567	X-ray diffractometry	1548:1567	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	2	56	theme	fragile	243:249	arg1	aerogels					251:258	fragile aerogels	243:258	fragile aerogels with poor shape control	243:282	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	5	57	from	rinsing	919:925	arg1	water					930:934	water	930:934	water	930:934	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	1	58	theme	cellulose	113:121	arg1	aerogels					166:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	7	59	with	suitable	1387:1394	arg1	diffusion					1278:1286	diffusion	1278:1286	diffusion as the basis to introduce and remove chemical species into and out of the hydrogel	1278:1369	With diffusion as the basis to introduce and remove chemical species into and out of the hydrogel, this method is suitable for smaller bulk geometries and thin films.
31132052	1	60	theme	biotemplated	133:144	arg1	aerogels					166:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	6	61	theme	pre-formed	1214:1223	arg1	nanoparticles					1225:1237	pre-formed nanoparticles	1214:1237	pre-formed nanoparticles used in other sol-gel techniques	1214:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	6	62	theme	final	1092:1096	arg1	composite					1106:1114	the final aerogel composite	1088:1114	the final aerogel composite	1088:1114	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	3	63	theme	nanofibers	324:333	arg1	use					289:291	The use	285:291	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel	285:377	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	64	theme	gravimetric	1578:1588	arg1	analysis					1590:1597	thermal gravimetric analysis	1570:1597	thermal gravimetric analysis	1570:1597	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	5	65	with	crosslinking	814:825	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	65	with	crosslinking	814:825	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	2	66	theme	shape	270:274	arg1	control					276:282	poor shape control	265:282	poor shape control	265:282	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	5	67	theme	solvent	945:951	arg1	exchange					953:960	ethanol solvent exchange	937:960	ethanol solvent exchange	937:960	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	1	68	theme	nanofiber	123:131	arg1	aerogels					166:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	cellulose nanofiber biotemplated palladium composite aerogels	113:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	7	69	theme	bulk	1408:1411	arg1	geometries					1413:1422	smaller bulk geometries	1400:1422	smaller bulk geometries	1400:1422	With diffusion as the basis to introduce and remove chemical species into and out of the hydrogel, this method is suitable for smaller bulk geometries and thin films.
31132052	4	70	dep	Crosslinking	552:563	arg1	nanofibers					597:606	the carboxymethylated cellulose nanofibers	565:606	Crosslinking the carboxymethylated cellulose nanofibers	552:606	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	5	71	from	water	930:934	arg1	rinsing					919:925	rinsing	919:925	rinsing	919:925	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	6	72	theme	metal	1071:1075	arg1	content					1077:1083	the metal content	1067:1083	the metal content in the final aerogel composite	1067:1114	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	8	73	theme	gas	1609:1611	arg1	adsorption					1613:1622	nitrogen gas adsorption	1600:1622	nitrogen gas adsorption	1600:1622	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	6	74	from	content	1077:1083	arg1	composite					1106:1114	the final aerogel composite	1088:1114	the final aerogel composite	1088:1114	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	1	75	dep	method	92:97	arg1	synthesize					102:111	synthesize	102:111	to synthesize cellulose nanofiber biotemplated palladium composite aerogels	99:173	Here, a method to synthesize cellulose nanofiber biotemplated palladium composite aerogels is presented.
31132052	5	76	theme	synthesis	779:787	arg1	drying					985:990	CO2 supercritical drying	967:990	CO2 supercritical drying	967:990	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	76	theme	synthesis	779:787	arg1	crosslinking					814:825	covalent crosslinking	805:825	covalent crosslinking	805:825	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	76	theme	synthesis	779:787	arg1	equilibration					828:840	equilibration	828:840	equilibration with precursor ions	828:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	76	theme	synthesis	779:787	arg1	exchange					953:960	ethanol solvent exchange	937:960	ethanol solvent exchange	937:960	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	76	theme	synthesis	779:787	arg1	reduction					869:877	metal reduction	863:877	metal reduction with high concentration reducing agent, rinsing in water	863:934	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	76	theme	synthesis	779:787	arg1	steps					789:793	synthesis steps	779:793	synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying	779:990	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	77	with	exchange	953:960	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	5	77	with	exchange	953:960	arg1	agent					912:916	high concentration reducing agent	884:916	high concentration reducing agent	884:916	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	3	78	theme	bonded	363:368	arg1	hydrogel					370:377	a covalently bonded hydrogel	350:377	a covalently bonded hydrogel	350:377	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	79	theme	impedance	1641:1649	arg1	spectroscopy					1651:1662	electrochemical impedance spectroscopy	1625:1662	electrochemical impedance spectroscopy	1625:1662	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	4	80	theme	3-dimethylaminopropyl	637:657	arg1	carbodiimide					660:671	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	626:671	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)	626:691	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	6	81	theme	ion	1025:1027	arg1	concentration					1029:1041	the precursor palladium ion concentration	1001:1041	the precursor palladium ion concentration	1001:1041	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	6	82	theme	ion	1133:1135	arg1	reduction					1146:1154	a direct ion chemical reduction	1124:1154	a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques	1124:1270	Varying the precursor palladium ion concentration allows for control over the metal content in the final aerogel composite through a direct ion chemical reduction rather than relying on the relatively slow coalescence of pre-formed nanoparticles used in other sol-gel techniques.
31132052	4	83	theme	cellulose	587:595	arg1	nanofibers					597:606	the carboxymethylated cellulose nanofibers	565:606	Crosslinking the carboxymethylated cellulose nanofibers	552:606	Crosslinking the carboxymethylated cellulose nanofibers is achieved using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of ethylenediamine.
31132052	0	84	theme	Cellulose	21:29	arg1	Aerogels					74:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	8	85	theme	cyclic	1669:1674	arg1	voltammetry					1676:1686	cyclic voltammetry	1669:1686	cyclic voltammetry	1669:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	2	86	theme	aerogel	201:207	arg1	methods					219:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	5	87	theme	precursor	847:855	arg1	ions					857:860	precursor ions	847:860	precursor ions	847:860	The CNF hydrogels maintain their shape throughout synthesis steps including covalent crosslinking, equilibration with precursor ions, metal reduction with high concentration reducing agent, rinsing in water, ethanol solvent exchange, and CO2 supercritical drying.
31132052	2	88	theme	Noble	189:193	arg1	methods					219:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods	189:225	Noble metal aerogel synthesis methods often result in fragile aerogels with poor shape control.
31132052	0	89	theme	Composite	64:72	arg1	Aerogels					74:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Cellulose Nanofiber Biotemplated Palladium Composite Aerogels	21:81	Synthesis Method for Cellulose Nanofiber Biotemplated Palladium Composite Aerogels.
31132052	3	90	theme	ions	413:416	arg1	reduction					394:402	the reduction	390:402	the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying	390:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	91	theme	high	1700:1703	arg1	structure					1747:1755	metallized palladium porous structure	1719:1755	metallized palladium porous structure	1719:1755	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	91	theme	high	1700:1703	arg1	area					1713:1716	a high surface area	1698:1716	a high surface area	1698:1716	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	92	theme	cellulose	1464:1472	arg1	aerogels					1504:1511	the cellulose nanofiber-palladium composite aerogels	1460:1511	the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry	1460:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	3	93	theme	aerogel	501:507	arg1	shape					518:522	macroscopic aerogel monolith shape	489:522	macroscopic aerogel monolith shape	489:522	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	8	94	theme	composite	1494:1502	arg1	aerogels					1504:1511	the cellulose nanofiber-palladium composite aerogels	1460:1511	the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry	1460:1686	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	3	95	from	palladium	426:434	arg1	CNFs					443:446	the CNFs	439:446	the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying	439:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31132052	7	96	theme	chemical	1325:1332	arg1	species					1334:1340	chemical species	1325:1340	chemical species	1325:1340	With diffusion as the basis to introduce and remove chemical species into and out of the hydrogel, this method is suitable for smaller bulk geometries and thin films.
31132052	8	97	theme	metallized	1719:1728	arg1	structure					1747:1755	metallized palladium porous structure	1719:1755	metallized palladium porous structure	1719:1755	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	8	97	theme	metallized	1719:1728	arg1	area					1713:1716	a high surface area	1698:1716	a high surface area	1698:1716	Characterization of the cellulose nanofiber-palladium composite aerogels with scanning electron microscopy, X-ray diffractometry, thermal gravimetric analysis, nitrogen gas adsorption, electrochemical impedance spectroscopy, and cyclic voltammetry indicates a high surface area, metallized palladium porous structure.
31132052	3	98	theme	supercritical	530:542	arg1	drying					544:549	supercritical drying	530:549	supercritical drying	530:549	The use of carboxymethylated cellulose nanofibers (CNFs) to form a covalently bonded hydrogel allows for the reduction of metal ions such as palladium on the CNFs with control over both nanostructure and macroscopic aerogel monolith shape after supercritical drying.
31002933	0	0	theme	antibacterial	22:34	arg1	potential					36:44	antibacterial potential	22:44	antibacterial potential	22:44	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	4	1	theme	prepared	637:644	arg1	films					646:650	The prepared films	633:650	The prepared films	633:650	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	2	2	theme	nanoparticles	371:383	arg1	synthesis					351:359	the in-situ synthesis	339:359	the in-situ synthesis of silver nanoparticles	339:383	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	5	3	dep	structural	812:821	arg1	XRD					824:826	XRD	824:826	XRD	824:826	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	5	4	theme	morphological	756:768	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	7	5	theme	composite	1098:1106	arg1	films					1108:1112	the composite films	1094:1112	the composite films	1094:1112	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	8	6	theme	good	1222:1225	arg1	profile					1236:1242	good swelling profile	1222:1242	good swelling profile	1222:1242	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	7	theme	prepared	1197:1204	arg1	films					1206:1210	The prepared films	1193:1210	RESULTS The prepared films	1185:1210	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	10	8	theme	collagen	1693:1700	arg1	reorganization					1702:1715	collagen reorganization	1693:1715	collagen reorganization	1693:1715	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	7	9	theme	healing	1074:1080	arg1	activity					1082:1089	The potential wound healing activity	1054:1089	The potential wound healing activity of the composite films	1054:1112	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	10	10	theme	mild	1742:1745	arg1	regeneration					1757:1768	mild epidermal regeneration	1742:1768	mild epidermal regeneration after 7 days of treatment with no silver ions	1742:1814	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	1	11	theme	chitosan-sericin-silver	202:224	arg1	films					247:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	6	12	theme	films	945:949	arg1	activity					924:931	The antibacterial activity	906:931	The antibacterial activity of the CSSN films	906:949	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	11	13	theme	prepared	1910:1917	arg1	dressing					1935:1942	the prepared novel composite dressing	1906:1942	the prepared novel composite dressing	1906:1942	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	7	14	theme	potential	1058:1066	arg1	healing					1074:1080	potential wound healing	1058:1080	The potential wound healing activity of the composite films	1054:1112	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	3	15	theme	zeta-size	609:617	arg1	spectrometer					619:630	zeta-size spectrometer	609:630	zeta-size spectrometer	609:630	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	9	16	theme	prepared	1401:1408	arg1	films					1410:1414	The prepared films	1397:1414	The prepared films	1397:1414	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	9	16	theme	prepared	1401:1408	arg1	films					1450:1454	the Mox-loaded CSSN films	1430:1454	the Mox-loaded CSSN films	1430:1454	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	11	17	theme	obtained	1860:1867	arg1	findings					1869:1876	The obtained findings	1856:1876	The obtained findings	1856:1876	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	8	18	theme	in-vitro	1261:1268	arg1	release					1274:1280	a sustained in-vitro Mox release	1249:1280	a sustained in-vitro Mox release	1249:1280	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	11	19	theme	composite	1925:1933	arg1	dressing					1935:1942	the prepared novel composite dressing	1906:1942	the prepared novel composite dressing	1906:1942	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	10	20	with	treatment	1786:1794	arg1	ions					1811:1814	no silver ions	1801:1814	no silver ions	1801:1814	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	5	21	dep	elemental	777:785	arg1	EDX					788:790	EDX	788:790	EDX	788:790	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	10	22	theme	regeneration	1757:1768	arg1	detection					1816:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection	1672:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood	1672:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	11	23	theme	wound	1948:1952	arg1	applications					1959:1970	wound care applications	1948:1970	wound care applications	1948:1970	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	8	24	theme	permeation	1286:1295	arg1	profile					1297:1303	permeation profile	1286:1303	permeation profile	1286:1303	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	10	25	theme	neovascularization	1718:1735	arg1	detection					1816:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection	1672:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood	1672:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	9	26	theme	CSSN	1445:1448	arg1	films					1410:1414	The prepared films	1397:1414	The prepared films	1397:1414	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	9	26	theme	CSSN	1445:1448	arg1	films					1450:1454	the Mox-loaded CSSN films	1430:1454	the Mox-loaded CSSN films	1430:1454	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	4	27	theme	permeation	731:740	arg1	analysis					742:749	permeation analysis	731:749	permeation analysis	731:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	27	theme	permeation	731:740	arg1	contents					684:691	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	0	28	theme	nanocomposite	66:78	arg1	films					106:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	10	29	theme	treatment	1786:1794	arg1	7 days					1776:1781	7 days	1776:1781	7 days of treatment with no silver ions	1776:1814	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	6	30	theme	bacterial	990:998	arg1	strains					1000:1006	seven pathogenic bacterial strains	973:1006	seven pathogenic bacterial strains including five ATCC and two clinical strains	973:1051	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	6	30	theme	bacterial	990:998	arg1	ATCC					1023:1026	five ATCC	1018:1026	five ATCC	1018:1026	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	6	30	theme	bacterial	990:998	arg1	strains					1045:1051	two clinical strains	1032:1051	two clinical strains	1032:1051	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	0	31	theme	chitosan-silver-sericin	81:103	arg1	films					106:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	5	32	theme	composites	874:883	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	11	33	theme	care	1954:1957	arg1	applications					1959:1970	wound care applications	1948:1970	wound care applications	1948:1970	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	0	34	theme	films	106:110	arg1	healing					10:16	wound healing	4:16	wound healing	4:16	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	0	34	theme	films	106:110	arg1	potential					36:44	antibacterial potential	22:44	antibacterial potential	22:44	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	1	35	theme	current	146:152	arg1	study					154:158	The current study	142:158	The current study	142:158	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	7	36	theme	male	1174:1177	arg1	rats					1179:1182	Sprague Dawley male rats	1159:1182	Sprague Dawley male rats	1159:1182	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	9	37	theme	tested	1517:1522	arg1	strains					1524:1530	all the tested strains	1509:1530	all the tested strains	1509:1530	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	2	38	theme	solvent	472:478	arg1	technique					488:496	solvent casting technique	472:496	solvent casting technique	472:496	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	11	39	dep	CONCLUSION	1845:1854	arg1	suggest					1887:1893	suggest	1887:1893	suggest the use of the prepared novel composite dressing for wound care applications	1887:1970	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	2	40	dep	METHODOLOGY	290:300	arg1	method					323:328	The film preparation method	302:328	METHODOLOGY The film preparation method	290:328	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	4	41	theme	Mox	714:716	arg1	release					718:724	in-vitro Mox release	705:724	in-vitro Mox release	705:724	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	41	theme	Mox	714:716	arg1	contents					684:691	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	7	42	theme	wound	1136:1140	arg1	model					1142:1146	burn wound model	1131:1146	burn wound model induced in Sprague Dawley male rats	1131:1182	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	3	43	theme	size	534:537	arg1	analysis					539:546	the particle size analysis	521:546	the particle size analysis of the silver nanoparticles	521:574	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	5	44	dep	thermal	834:840	arg1	DSC					851:853	DSC	851:853	DSC	851:853	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	5	44	dep	thermal	834:840	arg1	TGA					843:845	TGA	843:845	TGA	843:845	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	10	45	theme	wound	1640:1644	arg1	healing					1646:1652	a remarkable wound healing	1627:1652	a remarkable wound healing applications	1627:1665	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	1	46	theme	films	247:251	arg1	evaluation					188:197	evaluation	188:197	evaluation	188:197	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	1	46	theme	films	247:251	arg1	development					172:182	development	172:182	development	172:182	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	0	47	dep	healing	10:16	arg1	The					0:2	The	0:2	The	0:2	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	10	48	theme	prepared	1602:1609	arg1	films					1611:1615	The prepared films	1598:1615	The prepared films	1598:1615	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	3	49	theme	silver	555:560	arg1	nanoparticles					562:574	the silver nanoparticles	551:574	the silver nanoparticles	551:574	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	9	50	dep	highest	1556:1562	arg1	isolates					1587:1594	clinical isolates	1578:1594	clinical isolates	1578:1594	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	9	51	theme	clinical	1578:1585	arg1	isolates					1587:1594	clinical isolates	1578:1594	clinical isolates	1578:1594	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	7	52	theme	films	1108:1112	arg1	activity					1082:1089	The potential wound healing activity	1054:1089	The potential wound healing activity of the composite films	1054:1112	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	10	53	theme	successful	1672:1681	arg1	detection					1816:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection	1672:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood	1672:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	2	54	theme	film	306:309	arg1	method					323:328	The film preparation method	302:328	METHODOLOGY The film preparation method	290:328	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	2	55	theme	silver	364:369	arg1	nanoparticles					371:383	silver nanoparticles	364:383	silver nanoparticles	364:383	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	10	56	theme	reorganization	1702:1715	arg1	detection					1816:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection	1672:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood	1672:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	4	57	theme	in-vitro	705:712	arg1	release					718:724	in-vitro Mox release	705:724	in-vitro Mox release	705:724	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	57	theme	in-vitro	705:712	arg1	contents					684:691	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	5	58	theme	spectral	794:801	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	7	59	theme	wound	1068:1072	arg1	healing					1074:1080	potential wound healing	1058:1080	The potential wound healing activity of the composite films	1054:1112	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	10	60	theme	epidermal	1747:1755	arg1	regeneration					1757:1768	mild epidermal regeneration	1742:1768	mild epidermal regeneration after 7 days of treatment with no silver ions	1742:1814	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	10	61	theme	remarkable	1629:1638	arg1	healing					1646:1652	a remarkable wound healing	1627:1652	a remarkable wound healing applications	1627:1665	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	8	62	theme	swelling	1227:1234	arg1	profile					1236:1242	good swelling profile	1222:1242	good swelling profile	1222:1242	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	1	63	theme	nanocomposite	226:238	arg1	films					247:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	6	64	theme	CSSN	940:943	arg1	films					945:949	the CSSN films	936:949	the CSSN films	936:949	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	5	65	dep	spectral	794:801	arg1	FT-IR					804:808	FT-IR	804:808	FT-IR	804:808	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	9	66	theme	promising	1468:1476	arg1	activity					1492:1499	a promising antibacterial activity	1466:1499	a promising antibacterial activity against all the tested strains	1466:1530	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	11	67	theme	novel	1919:1923	arg1	dressing					1935:1942	the prepared novel composite dressing	1906:1942	the prepared novel composite dressing	1906:1942	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	5	68	theme	thermal	834:840	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	8	69	theme	sustained	1251:1259	arg1	release					1274:1280	a sustained in-vitro Mox release	1249:1280	a sustained in-vitro Mox release	1249:1280	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	10	70	theme	fibrosis	1683:1690	arg1	detection					1816:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection	1672:1824	successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood	1672:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	6	71	theme	antibacterial	910:922	arg1	activity					924:931	The antibacterial activity	906:931	The antibacterial activity of the CSSN films	906:949	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	8	72	theme	Mox	1270:1272	arg1	release					1274:1280	a sustained in-vitro Mox release	1249:1280	a sustained in-vitro Mox release	1249:1280	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	10	73	theme	silver	1804:1809	arg1	ions					1811:1814	no silver ions	1801:1814	no silver ions	1801:1814	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	9	74	theme	Mox-loaded	1434:1443	arg1	films					1410:1414	The prepared films	1397:1414	The prepared films	1397:1414	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	9	74	theme	Mox-loaded	1434:1443	arg1	films					1450:1454	the Mox-loaded CSSN films	1430:1454	the Mox-loaded CSSN films	1430:1454	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	2	75	dep	involved	330:337	arg1	followed					433:440	followed	433:440	followed by preparation into a film by solvent casting technique	433:496	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	11	76	theme	dressing	1935:1942	arg1	use					1899:1901	the use	1895:1901	the use of the prepared novel composite dressing for wound care applications	1895:1970	CONCLUSION The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.
31002933	3	77	theme	particle	525:532	arg1	analysis					539:546	the particle size analysis	521:546	the particle size analysis of the silver nanoparticles	521:574	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	6	78	theme	pathogenic	979:988	arg1	strains					1000:1006	seven pathogenic bacterial strains	973:1006	seven pathogenic bacterial strains including five ATCC and two clinical strains	973:1051	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	6	78	theme	pathogenic	979:988	arg1	ATCC					1023:1026	five ATCC	1018:1026	five ATCC	1018:1026	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	6	78	theme	pathogenic	979:988	arg1	strains					1045:1051	two clinical strains	1032:1051	two clinical strains	1032:1051	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	5	79	dep	morphological	756:768	arg1	SEM					771:773	SEM	771:773	SEM	771:773	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	9	80	theme	antibacterial	1478:1490	arg1	activity					1492:1499	a promising antibacterial activity	1466:1499	a promising antibacterial activity against all the tested strains	1466:1530	The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates).
31002933	2	81	theme	preparation	311:321	arg1	method					323:328	The film preparation method	302:328	METHODOLOGY The film preparation method	290:328	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	2	82	theme	in-situ	343:349	arg1	synthesis					351:359	the in-situ synthesis	339:359	the in-situ synthesis of silver nanoparticles	339:383	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	5	83	theme	elemental	777:785	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	1	84	dep	AIM	138:140	arg1	reports					160:166	reports	160:166	reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox)	160:287	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	7	85	theme	Dawley	1167:1172	arg1	rats					1179:1182	Sprague Dawley male rats	1159:1182	Sprague Dawley male rats	1159:1182	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	5	86	theme	structural	812:821	arg1	properties					856:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties	752:865	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites	752:883	The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected.
31002933	2	87	theme	casting	480:486	arg1	technique					488:496	solvent casting technique	472:496	solvent casting technique	472:496	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	1	88	dep	development	172:182	arg1	the					168:170	the	168:170	the	168:170	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	2	89	theme	colloidal	413:421	arg1	composite					423:431	the chitosan-sericin colloidal composite	392:431	the chitosan-sericin colloidal composite	392:431	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	6	90	theme	clinical	1036:1043	arg1	strains					1045:1051	two clinical strains	1032:1051	two clinical strains	1032:1051	The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains.
31002933	8	91	theme	release	1342:1348	arg1	CSSM1					1362:1366	CSSM1	1362:1366	CSSM1	1362:1366	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	91	theme	release	1342:1348	arg1	release					1342:1348	78.57% (CSSM3) release	1327:1348	78.57% (CSSM3) release	1327:1348	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	91	theme	release	1342:1348	arg1	%					1359:1359	55.05%	1354:1359	55.05% (CSSM1)	1354:1367	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	91	theme	release	1342:1348	arg1	maximum					1316:1322	maximum	1316:1322	maximum of 78.57% (CSSM3) release	1316:1348	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	2	92	theme	chitosan-sericin	396:411	arg1	composite					423:431	the chitosan-sericin colloidal composite	392:431	the chitosan-sericin colloidal composite	392:431	METHODOLOGY The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique.
31002933	7	93	theme	burn	1131:1134	arg1	model					1142:1146	burn wound model	1131:1146	burn wound model induced in Sprague Dawley male rats	1131:1182	The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats.
31002933	1	94	theme	CSSN	241:244	arg1	films					247:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	chitosan-sericin-silver nanocomposite (CSSN) films	202:251	AIM The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox).
31002933	8	95	dep	maximum	1316:1322	arg1	permeation					1369:1378	permeation	1369:1378	permeation (CSSM1)	1369:1386	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	95	dep	maximum	1316:1322	arg1	CSSM1					1381:1385	CSSM1	1381:1385	CSSM1	1381:1385	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	8	96	dep	RESULTS	1185:1191	arg1	films					1206:1210	The prepared films	1193:1210	RESULTS The prepared films	1185:1210	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	3	97	theme	In-situ	499:505	arg1	formation					507:515	In-situ formation	499:515	In-situ formation	499:515	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	0	98	theme	triple-component	49:64	arg1	films					106:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	triple-component nanocomposite (chitosan-silver-sericin) films	49:110	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
31002933	4	99	dep	contents	684:691	arg1	analysis					742:749	permeation analysis	731:749	permeation analysis	731:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	99	dep	contents	684:691	arg1	uniformity					693:702	uniformity	693:702	uniformity	693:702	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	99	dep	contents	684:691	arg1	release					718:724	in-vitro Mox release	705:724	in-vitro Mox release	705:724	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	4	99	dep	contents	684:691	arg1	contents					684:691	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	contents uniformity, in-vitro Mox release, and permeation analysis	684:749	The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis.
31002933	10	100	theme	healing	1646:1652	arg1	applications					1654:1665	a remarkable wound healing applications	1627:1665	a remarkable wound healing applications	1627:1665	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	8	101	theme	%	1332:1332	arg1	release					1342:1348	78.57% (CSSM3) release	1327:1348	78.57% (CSSM3) release	1327:1348	RESULTS The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h.
31002933	3	102	theme	nanoparticles	562:574	arg1	analysis					539:546	the particle size analysis	521:546	the particle size analysis of the silver nanoparticles	521:574	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	3	102	theme	nanoparticles	562:574	arg1	formation					507:515	In-situ formation	499:515	In-situ formation	499:515	In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer.
31002933	10	103	from	detection	1816:1824	arg1	blood					1838:1842	animal's blood	1829:1842	animal's blood	1829:1842	The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood.
31002933	0	104	theme	wound	4:8	arg1	healing					10:16	wound healing	4:16	wound healing	4:16	The wound healing and antibacterial potential of triple-component nanocomposite (chitosan-silver-sericin) films loaded with moxifloxacin.
30448748	2	0	theme	nZVI	541:544	arg1	size					533:536	the smaller particle size	512:536	the smaller particle size of nZVI on the surface of biochar	512:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	9	1	theme	adsorption	1457:1466	arg1	model					1468:1472	the Langmuir isothermal adsorption model	1433:1472	the Langmuir isothermal adsorption model	1433:1472	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	3	2	theme	surface	610:616	arg1	11.1 m2/g					644:652	11.1 m2/g	644:652	11.1 m2/g	644:652	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	3	2	theme	surface	610:616	arg1	area					618:621	the specific surface area	597:621	the specific surface area of the composite	597:638	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	4	3	theme	oxygen-containing	762:778	arg1	OH					806:807	OH	806:807	OH	806:807	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	3	theme	oxygen-containing	762:778	arg1	OCO					814:816	OCO	814:816	OCO	814:816	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	3	theme	oxygen-containing	762:778	arg1	-COOH					799:803	-COOH	799:803	-COOH	799:803	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	3	theme	oxygen-containing	762:778	arg1	groups					791:796	oxygen-containing functional groups	762:796	oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	762:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	3	4	theme	composite	630:638	arg1	11.1 m2/g					644:652	11.1 m2/g	644:652	11.1 m2/g	644:652	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	3	4	theme	composite	630:638	arg1	area					618:621	the specific surface area	597:621	the specific surface area of the composite	597:638	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	1	5	theme	zero-valent	167:177	arg1	iron					179:182	nano-scale zero-valent iron	156:182	nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC)	156:245	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	5	theme	zero-valent	167:177	arg1	biochar-CMC-nZVI					185:200	biochar-CMC-nZVI	185:200	biochar-CMC-nZVI	185:200	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	6	used	used	265:268	arg2	biochar					138:144	novel biochar	132:144	novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC)	132:245	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	9	7	theme	kinetic	1415:1421	arg1	model					1423:1427	The pseudo-second-order kinetic model	1391:1427	lower pH. The pseudo-second-order kinetic model	1381:1427	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	8	dep	Cr	1331:1332	arg1	VI					1334:1335	VI	1334:1335	VI	1334:1335	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	8	dep	Cr	1331:1332	arg1	removal					1338:1344	removal	1338:1344	Cr(VI) removal by biochar-CMC-nZVI	1331:1364	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	6	9	with	reaction	966:973	arg1	Cr					980:981	Cr	980:981	Cr(VI)	980:985	Upon reaction with Cr(VI), CrxFe1-x(OH)3 and FexCryO4 were deposited on the surface of biochar-CMC-nZVI composite.
30448748	10	10	theme	low-cost	1697:1704	arg1	sorbent					1730:1736	a low-cost, "green", and effective sorbent	1695:1736	a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment	1695:1777	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	5	11	theme	composite	881:889	arg1	material					891:898	the composite material	877:898	the composite material	877:898	Cr(VI) was reduced to Cr(III) by the composite material, mainly due to the reduction of nZVI on the biochar surface.
30448748	9	12	theme	Langmuir	1437:1444	arg1	adsorption					1457:1466	the Langmuir isothermal adsorption	1433:1466	the Langmuir isothermal adsorption model	1433:1472	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	7	13	theme	removal	1156:1162	arg1	complexation					1125:1136	surface complexation	1117:1136	surface complexation	1117:1136	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	13	theme	removal	1156:1162	arg1	reduction					1102:1110	reduction	1102:1110	reduction	1102:1110	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	13	theme	removal	1156:1162	arg1	attraction					1090:1099	Electrostatic attraction	1076:1099	Electrostatic attraction	1076:1099	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	13	theme	removal	1156:1162	arg1	mechanisms					1164:1173	the dominant removal mechanisms	1143:1173	the dominant removal mechanisms	1143:1173	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	4	14	theme	groups	791:796	arg1	number					752:757	a large number	744:757	a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	744:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	10	15	theme	"	1713:1713	arg1	sorbent					1730:1736	a low-cost, "green", and effective sorbent	1695:1736	a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment	1695:1777	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	6	16	theme	CrxFe1-x	988:995	arg1	OH					997:998	CrxFe1-x(OH)3	988:1000	CrxFe1-x(OH)3	988:1000	Upon reaction with Cr(VI), CrxFe1-x(OH)3 and FexCryO4 were deposited on the surface of biochar-CMC-nZVI composite.
30448748	3	17	theme	lower	655:659	arg1	11.1 m2/g					644:652	11.1 m2/g	644:652	11.1 m2/g	644:652	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	3	17	theme	lower	655:659	arg1	area					618:621	the specific surface area	597:621	the specific surface area of the composite	597:638	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	2	18	theme	biochar	447:453	arg1	surface					436:442	the surface	432:442	the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar	432:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	0	19	theme	iron	58:61	arg1	composite					63:71	nano zero-valent iron composite	41:71	nano zero-valent iron composite	41:71	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	1	20	theme	Cr	310:311	arg1	removal					278:284	the removal	274:284	the removal of hexavalent chromium (Cr(VI) from aqueous solution	274:337	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	21	theme	novel	132:136	arg1	biochar					138:144	novel biochar	132:144	novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC)	132:245	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	22	dep	Cr	310:311	arg1	VI					313:314	VI	313:314	VI	313:314	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	4	23	with	Fe	736:737	arg1	number					752:757	a large number	744:757	a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	744:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	10	24	theme	Cr	1753:1754	arg1	removal					1742:1748	removal	1742:1748	removal of Cr(VI)	1742:1758	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	0	25	theme	chromium	88:95	arg1	removal					97:103	hexavalent chromium removal	77:103	hexavalent chromium removal from water	77:114	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	2	26	theme	biochar	564:570	arg1	surface					553:559	the surface	549:559	the surface of biochar	549:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	9	27	theme	indicated	1531:1539	arg1	mechanism					1559:1567	adsorption mechanism	1548:1567	adsorption mechanism	1548:1567	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	27	theme	indicated	1531:1539	arg1	Cr					1541:1542	indicated Cr	1531:1542	indicated Cr	1531:1542	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	27	theme	indicated	1531:1539	arg1	adsorption					1607:1616	a chemisorption based multi-layer adsorption	1573:1616	a chemisorption based multi-layer adsorption	1573:1616	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	5	28	from	reduction	919:927	arg1	surface					952:958	the biochar surface	940:958	the biochar surface	940:958	Cr(VI) was reduced to Cr(III) by the composite material, mainly due to the reduction of nZVI on the biochar surface.
30448748	9	29	dep	adsorption	1607:1616	arg1	VI					1544:1545	VI	1544:1545	VI	1544:1545	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	8	30	theme	1.25 g/L	1296:1303	arg1	dosage					1286:1291	a dosage	1284:1291	a dosage of 1.25 g/L and an initial pH of 5.6	1284:1328	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	0	31	theme	biochar	8:14	arg1	CMC					26:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	7	32	theme	Electrostatic	1076:1088	arg1	complexation					1125:1136	surface complexation	1117:1136	surface complexation	1117:1136	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	32	theme	Electrostatic	1076:1088	arg1	reduction					1102:1110	reduction	1102:1110	reduction	1102:1110	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	32	theme	Electrostatic	1076:1088	arg1	attraction					1090:1099	Electrostatic attraction	1076:1099	Electrostatic attraction	1076:1099	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	32	theme	Electrostatic	1076:1088	arg1	mechanisms					1164:1173	the dominant removal mechanisms	1143:1173	the dominant removal mechanisms	1143:1173	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	5	33	theme	biochar	944:950	arg1	surface					952:958	the biochar surface	940:958	the biochar surface	940:958	Cr(VI) was reduced to Cr(III) by the composite material, mainly due to the reduction of nZVI on the biochar surface.
30448748	3	34	theme	pristine	674:681	arg1	biochar					683:689	pristine biochar	674:689	pristine biochar	674:689	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	8	35	theme	pH	1320:1321	arg1	dosage					1286:1291	a dosage	1284:1291	a dosage of 1.25 g/L and an initial pH of 5.6	1284:1328	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	2	36	dep	particles	376:384	arg1	80 nm					393:397	80 nm	393:397	80 nm	393:397	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	10	37	theme	biochar-CMC-nZVI	1665:1680	arg1	promise					1654:1660	the promise	1650:1660	the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment	1650:1777	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	0	38	theme	nano	41:44	arg1	composite					63:71	nano zero-valent iron composite	41:71	nano zero-valent iron composite	41:71	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	1	39	theme	chromium	300:307	arg1	Cr					310:311	hexavalent chromium (Cr(VI)	289:315	hexavalent chromium (Cr(VI)	289:315	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	8	40	theme	5.6	1326:1328	arg1	1.25 g/L					1296:1303	1.25 g/L	1296:1303	1.25 g/L	1296:1303	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	8	40	theme	5.6	1326:1328	arg1	pH					1320:1321	an initial pH	1309:1321	an initial pH of 5.6	1309:1328	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	3	41	theme	specific	601:608	arg1	11.1 m2/g					644:652	11.1 m2/g	644:652	11.1 m2/g	644:652	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	3	41	theme	specific	601:608	arg1	area					618:621	the specific surface area	597:621	the specific surface area of the composite	597:638	The results showed that the specific surface area of the composite was 11.1 m2/g, lower than that of pristine biochar.
30448748	4	42	theme	element	702:708	arg1	composition					710:720	The basic element composition	692:720	The basic element composition	692:720	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	42	theme	element	702:708	arg1	C					726:726	C	726:726	C	726:726	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	1	43	theme	carboxymethyl	217:229	arg1	CMC					242:244	CMC	242:244	CMC	242:244	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	43	theme	carboxymethyl	217:229	arg1	cellulose					231:239	carboxymethyl cellulose	217:239	carboxymethyl cellulose (CMC)	217:245	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	9	44	theme	based	1589:1593	arg1	mechanism					1559:1567	adsorption mechanism	1548:1567	adsorption mechanism	1548:1567	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	44	theme	based	1589:1593	arg1	Cr					1541:1542	indicated Cr	1531:1542	indicated Cr	1531:1542	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	44	theme	based	1589:1593	arg1	adsorption					1607:1616	a chemisorption based multi-layer adsorption	1573:1616	a chemisorption based multi-layer adsorption	1573:1616	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	4	45	with	C	726:726	arg1	number					752:757	a large number	744:757	a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	744:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	0	46	theme	novel	2:6	arg1	CMC					26:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	4	47	dep	groups	791:796	arg1	OH					806:807	OH	806:807	OH	806:807	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	47	dep	groups	791:796	arg1	OCO					814:816	OCO	814:816	OCO	814:816	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	47	dep	groups	791:796	arg1	-COOH					799:803	-COOH	799:803	-COOH	799:803	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	47	dep	groups	791:796	arg1	groups					791:796	oxygen-containing functional groups	762:796	oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	762:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	48	theme	large	746:750	arg1	number					752:757	a large number	744:757	a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	744:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	2	49	theme	particle	524:531	arg1	size					533:536	the smaller particle size	512:536	the smaller particle size of nZVI on the surface of biochar	512:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	5	50	dep	reduced	855:861	arg1	VI					847:848	VI	847:848	VI	847:848	Cr(VI) was reduced to Cr(III) by the composite material, mainly due to the reduction of nZVI on the biochar surface.
30448748	1	51	theme	nano-scale	156:165	arg1	iron					179:182	nano-scale zero-valent iron	156:182	nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC)	156:245	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	1	51	theme	nano-scale	156:165	arg1	biochar-CMC-nZVI					185:200	biochar-CMC-nZVI	185:200	biochar-CMC-nZVI	185:200	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	9	52	theme	pseudo-second-order	1395:1413	arg1	model					1423:1427	The pseudo-second-order kinetic model	1391:1427	lower pH. The pseudo-second-order kinetic model	1381:1427	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	2	53	theme	nZVI	491:494	arg1	aggregation					476:486	the aggregation	472:486	the aggregation of nZVI	472:494	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	10	54	dep	Cr	1753:1754	arg1	VI					1756:1757	VI	1756:1757	VI	1756:1757	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	8	55	theme	initial	1312:1318	arg1	pH					1320:1321	an initial pH	1309:1321	an initial pH of 5.6	1309:1328	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	9	56	theme	isothermal	1446:1455	arg1	adsorption					1457:1466	the Langmuir isothermal adsorption	1433:1466	the Langmuir isothermal adsorption model	1433:1472	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	2	57	from	surface	553:559	arg1	size					533:536	the smaller particle size	512:536	the smaller particle size of nZVI on the surface of biochar	512:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	8	58	dep	Cr	1213:1214	arg1	VI					1216:1217	VI	1216:1217	VI	1216:1217	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	4	59	theme	functional	780:789	arg1	OH					806:807	OH	806:807	OH	806:807	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	59	theme	functional	780:789	arg1	OCO					814:816	OCO	814:816	OCO	814:816	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	59	theme	functional	780:789	arg1	-COOH					799:803	-COOH	799:803	-COOH	799:803	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	59	theme	functional	780:789	arg1	groups					791:796	oxygen-containing functional groups	762:796	oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	762:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	0	60	from	water	110:114	arg1	removal					97:103	hexavalent chromium removal	77:103	hexavalent chromium removal from water	77:114	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	7	61	theme	dominant	1147:1154	arg1	complexation					1125:1136	surface complexation	1117:1136	surface complexation	1117:1136	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	61	theme	dominant	1147:1154	arg1	reduction					1102:1110	reduction	1102:1110	reduction	1102:1110	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	61	theme	dominant	1147:1154	arg1	attraction					1090:1099	Electrostatic attraction	1076:1099	Electrostatic attraction	1076:1099	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	61	theme	dominant	1147:1154	arg1	mechanisms					1164:1173	the dominant removal mechanisms	1143:1173	the dominant removal mechanisms	1143:1173	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	6	62	theme	composite	1065:1073	arg1	surface					1037:1043	the surface	1033:1043	the surface of biochar-CMC-nZVI composite	1033:1073	Upon reaction with Cr(VI), CrxFe1-x(OH)3 and FexCryO4 were deposited on the surface of biochar-CMC-nZVI composite.
30448748	2	63	theme	smaller	516:522	arg1	size					533:536	the smaller particle size	512:536	the smaller particle size of nZVI on the surface of biochar	512:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	4	64	with	O	729:729	arg1	number					752:757	a large number	744:757	a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	744:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	7	65	theme	surface	1117:1123	arg1	complexation					1125:1136	surface complexation	1117:1136	surface complexation	1117:1136	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	65	theme	surface	1117:1123	arg1	reduction					1102:1110	reduction	1102:1110	reduction	1102:1110	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	65	theme	surface	1117:1123	arg1	attraction					1090:1099	Electrostatic attraction	1076:1099	Electrostatic attraction	1076:1099	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	7	65	theme	surface	1117:1123	arg1	mechanisms					1164:1173	the dominant removal mechanisms	1143:1173	the dominant removal mechanisms	1143:1173	Electrostatic attraction, reduction, and surface complexation were the dominant removal mechanisms.
30448748	4	66	located	observed	819:826	arg1	surface					835:841	the surface	831:841	the surface	831:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	66	located	observed	819:826	arg2	OH					806:807	OH	806:807	OH	806:807	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	66	located	observed	819:826	arg2	-COOH					799:803	-COOH	799:803	-COOH	799:803	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	66	located	observed	819:826	arg2	groups					791:796	oxygen-containing functional groups	762:796	oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface	762:841	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	66	located	observed	819:826	arg2	OCO					814:816	OCO	814:816	OCO	814:816	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	10	67	theme	effective	1720:1728	arg1	sorbent					1730:1736	a low-cost, "green", and effective sorbent	1695:1736	a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment	1695:1777	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	0	68	theme	hexavalent	77:86	arg1	removal					97:103	hexavalent chromium removal	77:103	hexavalent chromium removal from water	77:114	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	5	69	theme	nZVI	932:935	arg1	reduction					919:927	the reduction	915:927	the reduction of nZVI on the biochar surface	915:958	Cr(VI) was reduced to Cr(III) by the composite material, mainly due to the reduction of nZVI on the biochar surface.
30448748	9	70	theme	isotherm	1516:1523	arg1	data					1525:1528	the sorption kinetic and isotherm data	1491:1528	data	1525:1528	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	1	71	theme	aqueous	322:328	arg1	solution					330:337	aqueous solution	322:337	aqueous solution	322:337	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	9	72	dep	lower	1381:1385	arg1	pH.					1387:1389	lower pH. The pseudo-second-order kinetic model	1381:1427	lower pH. The pseudo-second-order kinetic model	1381:1427	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	72	dep	lower	1381:1385	arg1	model					1423:1427	The pseudo-second-order kinetic model	1391:1427	lower pH. The pseudo-second-order kinetic model	1381:1427	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	2	73	from	size	533:536	arg1	surface					553:559	the surface	549:559	the surface of biochar	549:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	0	74	theme	supported	16:24	arg1	CMC					26:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC	0:28	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	10	75	from	sorbent	1730:1736	arg1	environment					1767:1777	the environment	1763:1777	the environment	1763:1777	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	2	76	from	nZVI	541:544	arg1	surface					553:559	the surface	549:559	the surface of biochar	549:570	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	2	77	theme	nZVI	371:374	arg1	particles					376:384	nZVI particles	371:384	nZVI particles (about 80 nm)	371:398	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	9	78	theme	adsorption	1548:1557	arg1	mechanism					1559:1567	adsorption mechanism	1548:1567	adsorption mechanism	1548:1567	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	78	theme	adsorption	1548:1557	arg1	Cr					1541:1542	indicated Cr	1531:1542	indicated Cr	1531:1542	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	78	theme	adsorption	1548:1557	arg1	adsorption					1607:1616	a chemisorption based multi-layer adsorption	1573:1616	a chemisorption based multi-layer adsorption	1573:1616	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	2	79	theme	CMC	366:368	arg1	stabilization					349:361	the stabilization	345:361	the stabilization of CMC	345:368	With the stabilization of CMC, nZVI particles (about 80 nm) were effectively dispersed onto the surface of biochar, which inhibited the aggregation of nZVI and resulted in the smaller particle size of nZVI on the surface of biochar.
30448748	0	80	theme	zero-valent	46:56	arg1	composite					63:71	nano zero-valent iron composite	41:71	nano zero-valent iron composite	41:71	A novel biochar supported CMC stabilized nano zero-valent iron composite for hexavalent chromium removal from water.
30448748	1	81	theme	hexavalent	289:298	arg1	Cr					310:311	hexavalent chromium (Cr(VI)	289:315	hexavalent chromium (Cr(VI)	289:315	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
30448748	10	82	theme	present	1623:1629	arg1	study					1631:1635	The present study	1619:1635	The present study	1619:1635	The present study demonstrated the promise of biochar-CMC-nZVI composite as a low-cost, "green", and effective sorbent for removal of Cr(VI) in the environment.
30448748	9	83	theme	chemisorption	1575:1587	arg1	mechanism					1559:1567	adsorption mechanism	1548:1567	adsorption mechanism	1548:1567	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	83	theme	chemisorption	1575:1587	arg1	Cr					1541:1542	indicated Cr	1531:1542	indicated Cr	1531:1542	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	83	theme	chemisorption	1575:1587	arg1	adsorption					1607:1616	a chemisorption based multi-layer adsorption	1573:1616	a chemisorption based multi-layer adsorption	1573:1616	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	4	84	theme	basic	696:700	arg1	composition					710:720	The basic element composition	692:720	The basic element composition	692:720	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	4	84	theme	basic	696:700	arg1	C					726:726	C	726:726	C	726:726	The basic element composition was C, O, and Fe with a large number of oxygen-containing functional groups (-COOH, OH, and OCO) observed on the surface.
30448748	6	85	dep	Cr	980:981	arg1	VI					983:984	VI	983:984	VI	983:984	Upon reaction with Cr(VI), CrxFe1-x(OH)3 and FexCryO4 were deposited on the surface of biochar-CMC-nZVI composite.
30448748	9	86	theme	multi-layer	1595:1605	arg1	mechanism					1559:1567	adsorption mechanism	1548:1567	adsorption mechanism	1548:1567	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	86	theme	multi-layer	1595:1605	arg1	Cr					1541:1542	indicated Cr	1531:1542	indicated Cr	1531:1542	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	9	86	theme	multi-layer	1595:1605	arg1	adsorption					1607:1616	a chemisorption based multi-layer adsorption	1573:1616	a chemisorption based multi-layer adsorption	1573:1616	Cr(VI) removal by biochar-CMC-nZVI was favored by lower pH. The pseudo-second-order kinetic model and the Langmuir isothermal adsorption model fitted well with the sorption kinetic and isotherm data, indicated Cr(VI) adsorption mechanism was a chemisorption based multi-layer adsorption.
30448748	8	87	theme	100 mg/L	1204:1211	arg1	Cr					1213:1214	the 100 mg/L Cr	1200:1214	the 100 mg/L Cr(VI)	1200:1218	The results showed that the 100 mg/L Cr(VI) could be removed completely by biochar-CMC-nZVI within 18 h, at a dosage of 1.25 g/L and an initial pH of 5.6.
30448748	1	88	from	solution	330:337	arg1	removal					278:284	the removal	274:284	the removal of hexavalent chromium (Cr(VI) from aqueous solution	274:337	In this study, novel biochar supported nano-scale zero-valent iron (biochar-CMC-nZVI) stabilized by carboxymethyl cellulose (CMC) was developed and used for the removal of hexavalent chromium (Cr(VI) from aqueous solution.
29577296	9	0	theme	oral	1722:1725	arg1	diseases					1727:1734	oral diseases	1722:1734	oral diseases	1722:1734	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	6	1	dep	RESULTS	973:979	arg1	acid					1051:1054	succinic acid	1042:1054	succinic acid	1042:1054	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	1	dep	RESULTS	973:979	arg1	substrates					1007:1016	Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	981:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	973:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	1	dep	RESULTS	973:979	arg1	Met-Pro					1060:1066	Met-Pro	1060:1066	Met-Pro	1060:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	1	dep	RESULTS	973:979	arg1	N-acetyl-D-mannosamine					1018:1039	N-acetyl-D-mannosamine	1018:1039	N-acetyl-D-mannosamine	1018:1039	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	4	2	theme	model	622:626	arg1	species					643:649	14 model oral bacterial species	619:649	14 model oral bacterial species	619:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	7	3	theme	solution	1153:1160	arg1	pH					1133:1134	the pH	1129:1134	the pH of the prebiotic solution	1129:1160	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	9	4	theme	oral	1748:1751	arg1	health					1753:1758	oral health	1748:1758	oral health	1748:1758	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	2	5	theme	beneficial	286:295	arg1	bacteria					297:304	beneficial bacteria	286:304	beneficial bacteria	286:304	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	6	6	theme	potential	987:995	arg1	acid					1051:1054	succinic acid	1042:1054	succinic acid	1042:1054	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	6	theme	potential	987:995	arg1	substrates					1007:1016	Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	981:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	973:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	6	theme	potential	987:995	arg1	Met-Pro					1060:1066	Met-Pro	1060:1066	Met-Pro	1060:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	6	theme	potential	987:995	arg1	N-acetyl-D-mannosamine					1018:1039	N-acetyl-D-mannosamine	1018:1039	N-acetyl-D-mannosamine	1018:1039	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	8	7	theme	1.0	1525:1527	arg1	N-acetyl-D-mannosamine					1499:1520	N-acetyl-D-mannosamine	1499:1520	N-acetyl-D-mannosamine of 1.0 and 1.5 M	1499:1537	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	2	8	theme	dual	221:224	arg1	assays					246:251	dual species competition assays	221:251	dual species competition assays	221:251	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	5	9	theme	oxygen	871:876	arg1	concentration					878:890	oxygen concentration	871:890	oxygen concentration	871:890	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	8	10	theme	1.5 M	1533:1537	arg1	N-acetyl-D-mannosamine					1499:1520	N-acetyl-D-mannosamine	1499:1520	N-acetyl-D-mannosamine of 1.0 and 1.5 M	1499:1537	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	4	11	contain	containing	608:617	arg1	culture					600:606	A chemostat culture	588:606	A chemostat culture containing 14 model oral bacterial species	588:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	4	11	contain	containing	608:617	arg2	species					643:649	14 model oral bacterial species	619:649	14 model oral bacterial species	619:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	5	12	theme	prebiotic	934:942	arg1	substances					944:953	the prebiotic substances	930:953	the prebiotic substances	930:953	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	7	13	theme	prebiotic	1421:1429	arg1	substrate					1431:1439	the most promising oral prebiotic substrate	1397:1439	the most promising oral prebiotic substrate	1397:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	13	theme	prebiotic	1421:1429	arg1	N-acetyl-D-mannosamine					1371:1392	N-acetyl-D-mannosamine	1371:1392	N-acetyl-D-mannosamine	1371:1392	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	8	14	theme	clear	1452:1456	arg1	effect					1473:1478	a clear dose dependent effect	1450:1478	a clear dose dependent effect	1450:1478	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	8	15	theme	beneficial	1580:1589	arg1	species					1591:1597	97% beneficial species	1576:1597	97% beneficial species	1576:1597	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	8	16	theme	biofilm	1553:1559	arg1	composition					1561:1571	a biofilm composition	1551:1571	a biofilm composition of 97% beneficial species	1551:1597	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	7	17	theme	promising	1406:1414	arg1	substrate					1431:1439	the most promising oral prebiotic substrate	1397:1439	the most promising oral prebiotic substrate	1397:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	17	theme	promising	1406:1414	arg1	N-acetyl-D-mannosamine					1371:1392	N-acetyl-D-mannosamine	1371:1392	N-acetyl-D-mannosamine	1371:1392	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	18	theme	concentration	1260:1272	arg1	interplay					1217:1225	the interplay	1213:1225	the interplay of nutrient availability, oxygen concentration and prebiotic treatment	1213:1296	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	2	19	theme	competition	234:244	arg1	assays					246:251	dual species competition assays	221:251	dual species competition assays	221:251	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	4	20	used	used	655:658	arg2	culture					600:606	A chemostat culture	588:606	A chemostat culture containing 14 model oral bacterial species	588:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	9	21	from	concept	1637:1643	arg1	health					1653:1658	oral health	1648:1658	oral health	1648:1658	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	4	22	theme	prebiotic	727:735	arg1	solutions					737:745	prebiotic solutions	727:745	prebiotic solutions	727:745	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	3	23	from	effect	400:405	arg1	cultures					479:486	multispecies cultures	466:486	multispecies cultures	466:486	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	8	24	theme	%	1578:1578	arg1	species					1591:1597	97% beneficial species	1576:1597	97% beneficial species	1576:1597	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	7	25	theme	nutrient	1230:1237	arg1	availability					1239:1250	nutrient availability	1230:1250	nutrient availability	1230:1250	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	9	26	theme	prebiotic	1627:1635	arg1	concept					1637:1643	the prebiotic concept	1623:1643	the prebiotic concept in oral health	1623:1658	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	8	27	theme	species	1591:1597	arg1	composition					1561:1571	a biofilm composition	1551:1571	a biofilm composition of 97% beneficial species	1551:1597	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	3	28	theme	potential	432:440	arg1	substrates					452:461	previously identified potential prebiotic substrates	410:461	previously identified potential prebiotic substrates	410:461	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	4	29	dep	METHODS	580:586	arg1	used					655:658	used	655:658	was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days	651:786	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	0	30	from	influence	24:32	arg1	vitro					65:69	vitro	65:69	vitro	65:69	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	0	31	theme	Oral	0:3	arg1	prebiotics					5:14	Oral prebiotics	0:14	Oral prebiotics	0:14	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	6	32	dep	substrates	1007:1016	arg1	acid					1051:1054	succinic acid	1042:1054	succinic acid	1042:1054	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	32	dep	substrates	1007:1016	arg1	substrates					1007:1016	Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	981:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	973:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	32	dep	substrates	1007:1016	arg1	Met-Pro					1060:1066	Met-Pro	1060:1066	Met-Pro	1060:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	32	dep	substrates	1007:1016	arg1	N-acetyl-D-mannosamine					1018:1039	N-acetyl-D-mannosamine	1018:1039	N-acetyl-D-mannosamine	1018:1039	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	4	33	dep	times	753:757	arg1	day					761:763	a day	759:763	three times a day for 3 consecutive days	747:786	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	6	34	theme	to > 95	1113:1119	arg1	%					1120:1120	%	1120:1120	%	1120:1120	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	4	35	theme	consecutive	771:781	arg1	days					783:786	3 consecutive days	769:786	3 consecutive days	769:786	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	3	36	theme	multispecies	466:477	arg1	cultures					479:486	multispecies cultures	466:486	multispecies cultures	466:486	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	7	37	theme	prebiotic	1195:1203	arg1	effect					1205:1210	the prebiotic effect	1191:1210	the prebiotic effect	1191:1210	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	3	38	theme	beneficial	539:548	arg1	species					550:556	a more beneficial species	532:556	a more beneficial species	532:556	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	6	39	theme	beneficial	1091:1100	arg1	proportion					1102:1111	the beneficial proportion	1087:1111	the beneficial proportion to > 95%	1087:1120	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	2	40	theme	potential	163:171	arg1	prebiotics					178:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	8	41	theme	dependent	1463:1471	arg1	effect					1473:1478	a clear dose dependent effect	1450:1478	a clear dose dependent effect	1450:1478	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	2	42	from	effect	276:281	arg1	bacteria					297:304	beneficial bacteria	286:304	beneficial bacteria	286:304	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	2	42	from	effect	276:281	arg1	suppressing					318:328	suppressing	318:328	suppressing the outgrowth of pathogenic species	318:364	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	3	43	theme	substrates	452:461	arg1	effect					400:405	the effect	396:405	the effect of previously identified potential prebiotic substrates on multispecies cultures	396:486	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	9	44	theme	valid	1675:1679	arg1	approach					1681:1688	a valid approach	1673:1688	a valid approach for treatment and prevention of oral diseases	1673:1734	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	7	45	theme	microbial	1337:1345	arg1	composition					1347:1357	the microbial composition	1333:1357	the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate	1333:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	5	46	theme	factors	828:834	arg1	influence					801:809	Further the influence	789:809	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances	789:953	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	7	47	theme	significant	1310:1320	arg1	changes					1322:1328	significant changes	1310:1328	significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate	1310:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	4	48	theme	oral	628:631	arg1	species					643:649	14 model oral bacterial species	619:649	14 model oral bacterial species	619:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	5	49	theme	environmental	814:826	arg1	factors					828:834	environmental factors	814:834	environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose	814:909	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	5	49	theme	environmental	814:826	arg1	pH					844:845	pH	844:845	pH	844:845	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	5	49	theme	environmental	814:826	arg1	availability					857:868	nutrient availability	848:868	nutrient availability	848:868	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	5	49	theme	environmental	814:826	arg1	concentration					878:890	oxygen concentration	871:890	oxygen concentration	871:890	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	5	49	theme	environmental	814:826	arg1	dose					906:909	prebiotic dose	896:909	prebiotic dose	896:909	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	2	50	theme	pathogenic	347:356	arg1	species					358:364	pathogenic species	347:364	pathogenic species	347:364	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	9	51	theme	diseases	1727:1734	arg1	prevention					1708:1717	prevention	1708:1717	prevention	1708:1717	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	9	51	theme	diseases	1727:1734	arg1	treatment					1694:1702	treatment	1694:1702	treatment	1694:1702	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	7	52	theme	prebiotic	1278:1286	arg1	treatment					1288:1296	prebiotic treatment	1278:1296	prebiotic treatment	1278:1296	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	53	theme	prebiotic	1143:1151	arg1	solution					1153:1160	the prebiotic solution	1139:1160	the prebiotic solution	1139:1160	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	54	contain	have	1170:1173	arg1	pH					1133:1134	the pH	1129:1134	the pH of the prebiotic solution	1129:1160	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	54	contain	have	1170:1173	arg2	influence					1178:1186	an influence	1175:1186	an influence on the prebiotic effect	1175:1210	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	2	55	theme	stimulatory	264:274	arg1	effect					276:281	a stimulatory effect	262:281	a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species	262:364	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	0	56	theme	environmental	37:49	arg1	conditions					51:60	environmental conditions	37:60	environmental conditions	37:60	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	5	57	theme	prebiotic	896:904	arg1	dose					906:909	prebiotic dose	896:909	prebiotic dose	896:909	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	7	58	from	influence	1178:1186	arg1	effect					1205:1210	the prebiotic effect	1191:1210	the prebiotic effect	1191:1210	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	5	59	theme	nutrient	848:855	arg1	availability					857:868	nutrient availability	848:868	nutrient availability	848:868	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	6	60	theme	prebiotic	997:1005	arg1	acid					1051:1054	succinic acid	1042:1054	succinic acid	1042:1054	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	60	theme	prebiotic	997:1005	arg1	substrates					1007:1016	Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	981:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	973:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	60	theme	prebiotic	997:1005	arg1	Met-Pro					1060:1066	Met-Pro	1060:1066	Met-Pro	1060:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	60	theme	prebiotic	997:1005	arg1	N-acetyl-D-mannosamine					1018:1039	N-acetyl-D-mannosamine	1018:1039	N-acetyl-D-mannosamine	1018:1039	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	2	61	theme	species	226:232	arg1	assays					246:251	dual species competition assays	221:251	dual species competition assays	221:251	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	5	62	theme	substances	944:953	arg1	efficacy					918:925	the efficacy	914:925	the efficacy of the prebiotic substances	914:953	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	7	63	theme	oxygen	1253:1258	arg1	concentration					1260:1272	oxygen concentration	1253:1272	oxygen concentration	1253:1272	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	1	64	theme	prebiotics	112:121	arg1	concept					101:107	the concept	97:107	the concept of prebiotics	97:121	BACKGROUND Only recently the concept of prebiotics has been introduced in oral health.
29577296	8	65	theme	97	1576:1577	arg1	%					1578:1578	%	1578:1578	%	1578:1578	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	7	66	theme	oral	1416:1419	arg1	substrate					1431:1439	the most promising oral prebiotic substrate	1397:1439	the most promising oral prebiotic substrate	1397:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	66	theme	oral	1416:1419	arg1	N-acetyl-D-mannosamine					1371:1392	N-acetyl-D-mannosamine	1371:1392	N-acetyl-D-mannosamine	1371:1392	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	7	67	theme	availability	1239:1250	arg1	interplay					1217:1225	the interplay	1213:1225	the interplay of nutrient availability, oxygen concentration and prebiotic treatment	1213:1296	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	1	68	theme	oral	146:149	arg1	health					151:156	oral health	146:156	oral health	146:156	BACKGROUND Only recently the concept of prebiotics has been introduced in oral health.
29577296	0	69	from	prebiotics	5:14	arg1	vitro					65:69	vitro	65:69	vitro	65:69	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	6	70	theme	succinic	1042:1049	arg1	acid					1051:1054	succinic acid	1042:1054	succinic acid	1042:1054	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	6	70	theme	succinic	1042:1049	arg1	substrates					1007:1016	Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	981:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro	973:1066	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	3	71	theme	prebiotic	442:450	arg1	substrates					452:461	previously identified potential prebiotic substrates	410:461	previously identified potential prebiotic substrates	410:461	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	9	72	theme	oral	1648:1651	arg1	health					1653:1658	oral health	1648:1658	oral health	1648:1658	CONCLUSION Introducing the prebiotic concept in oral health might reveal a valid approach for treatment and prevention of oral diseases and promote oral health.
29577296	6	73	theme	%	1120:1120	arg1	proportion					1102:1111	the beneficial proportion	1087:1111	the beneficial proportion to > 95%	1087:1120	RESULTS Three potential prebiotic substrates N-acetyl-D-mannosamine, succinic acid and Met-Pro were able to bring the beneficial proportion to > 95%.
29577296	3	74	theme	identified	421:430	arg1	substrates					452:461	previously identified potential prebiotic substrates	410:461	previously identified potential prebiotic substrates	410:461	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	7	75	theme	composition	1347:1357	arg1	changes					1322:1328	significant changes	1310:1328	significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate	1310:1439	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	4	76	theme	chemostat	590:598	arg1	culture					600:606	A chemostat culture	588:606	A chemostat culture containing 14 model oral bacterial species	588:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	2	77	theme	oral	173:176	arg1	prebiotics					178:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	2	78	theme	Few	159:161	arg1	prebiotics					178:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics	159:187	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
29577296	0	79	theme	conditions	51:60	arg1	prebiotics					5:14	Oral prebiotics	0:14	Oral prebiotics	0:14	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	0	79	theme	conditions	51:60	arg1	influence					24:32	the influence	20:32	the influence of environmental conditions in vitro	20:69	Oral prebiotics and the influence of environmental conditions in vitro.
29577296	3	80	theme	biofilm	504:510	arg1	composition					512:522	the biofilm composition	500:522	the biofilm composition towards a more beneficial species dominated microbiota	500:577	This study aimed to validate the effect of previously identified potential prebiotic substrates on multispecies cultures by shifting the biofilm composition towards a more beneficial species dominated microbiota.
29577296	4	81	theme	bacterial	633:641	arg1	species					643:649	14 model oral bacterial species	619:649	14 model oral bacterial species	619:649	METHODS A chemostat culture containing 14 model oral bacterial species was used to grow biofilms for 24 hours which subsequently were treated with prebiotic solutions three times a day for 3 consecutive days.
29577296	7	82	theme	treatment	1288:1296	arg1	interplay					1217:1225	the interplay	1213:1225	the interplay of nutrient availability, oxygen concentration and prebiotic treatment	1213:1296	While the pH of the prebiotic solution did not have an influence on the prebiotic effect, the interplay of nutrient availability, oxygen concentration and prebiotic treatment resulted in significant changes of the microbial composition identifying N-acetyl-D-mannosamine as the most promising oral prebiotic substrate.
29577296	5	83	from	influence	801:809	arg1	efficacy					918:925	the efficacy	914:925	the efficacy of the prebiotic substances	914:953	Further the influence of environmental factors such as pH, nutrient availability, oxygen concentration and prebiotic dose on the efficacy of the prebiotic substances was investigated.
29577296	8	84	theme	N-acetyl-D-mannosamine	1499:1520	arg1	concentrations					1481:1494	concentrations	1481:1494	concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M	1481:1537	Showing a clear dose dependent effect, concentrations of N-acetyl-D-mannosamine of 1.0 and 1.5 M resulted in a biofilm composition of 97% beneficial species.
29577296	2	85	theme	species	358:364	arg1	outgrowth					334:342	the outgrowth	330:342	the outgrowth of pathogenic species	330:364	Few potential oral prebiotics have already been identified in dual species competition assays, showing a stimulatory effect on beneficial bacteria and by this suppressing the outgrowth of pathogenic species.
30114423	0	0	theme	films	80:84	arg1	microstructure					30:43	microstructure	30:43	microstructure	30:43	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	0	0	theme	films	80:84	arg1	properties					57:66	thermal properties	49:66	thermal properties	49:66	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	2	1	theme	chitosan	447:454	arg1	molecules					456:464	chitosan molecules	447:464	chitosan molecules	447:464	It showed that the addition of sorbitol weakened the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films.
30114423	0	2	theme	chitosan	71:78	arg1	films					80:84	chitosan films	71:84	chitosan films	71:84	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	3	3	theme	chitosan	646:653	arg1	crystallization					627:641	the crystallization	623:641	the crystallization of chitosan	623:653	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	2	4	theme	electrostatic	481:493	arg1	interactions					495:506	the electrostatic interactions	477:506	the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films	418:532	It showed that the addition of sorbitol weakened the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films.
30114423	0	5	from	Effect	0:5	arg1	microstructure					30:43	microstructure	30:43	microstructure	30:43	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	0	5	from	Effect	0:5	arg1	properties					57:66	thermal properties	49:66	thermal properties	49:66	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	5	6	theme	glass	944:948	arg1	transitions					950:960	two glass transitions	940:960	two glass transitions	940:960	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	7	7	theme	films	1248:1252	arg1	microstructure					1183:1196	the microstructure	1179:1196	the microstructure as well as properties of the plasticized chitosan films	1179:1252	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	7	7	theme	films	1248:1252	arg1	properties					1209:1218	properties	1209:1218	the microstructure as well as properties of the plasticized chitosan films	1179:1252	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	5	8	theme	sorbitol	870:877	arg1	content					879:885	the sorbitol content	866:885	the sorbitol content	866:885	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	7	9	theme	packaging	1330:1338	arg1	materials					1340:1348	packaging materials	1330:1348	packaging materials	1330:1348	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	7	9	theme	packaging	1330:1338	arg1	sorbitol					1282:1289	sorbitol	1282:1289	sorbitol	1282:1289	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	5	10	attach	presented	962:970	arg2	transitions					950:960	two glass transitions	940:960	two glass transitions	940:960	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	5	10	attach	presented	962:970	arg1	films					985:989	composite films	975:989	composite films	975:989	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	6	11	theme	temperature	1002:1012	arg1	transition					1014:1023	The lower temperature transition	992:1023	The lower temperature transition	992:1023	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	1	12	theme	Differential	328:339	arg1	DSC					363:365	DSC	363:365	DSC	363:365	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	12	theme	Differential	328:339	arg1	calorimetry					350:360	Differential scanning calorimetry	328:360	Differential scanning calorimetry (DSC)	328:366	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	13	from	influence	105:113	arg1	microstructure					142:155	microstructure	142:155	microstructure	142:155	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	13	from	influence	105:113	arg1	properties					169:178	thermal properties	161:178	thermal properties	161:178	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	6	14	theme	lower	996:1000	arg1	transition					1014:1023	The lower temperature transition	992:1023	The lower temperature transition	992:1023	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	2	15	theme	sorbitol	400:407	arg1	addition					388:395	the addition	384:395	the addition of sorbitol	384:407	It showed that the addition of sorbitol weakened the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films.
30114423	1	16	theme	scanning	341:348	arg1	DSC					363:365	DSC	363:365	DSC	363:365	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	16	theme	scanning	341:348	arg1	calorimetry					350:360	Differential scanning calorimetry	328:360	Differential scanning calorimetry (DSC)	328:366	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	3	17	theme	Structural	535:544	arg1	analysis					546:553	Structural analysis	535:553	Structural analysis	535:553	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	1	18	theme	thermal	161:167	arg1	properties					169:178	thermal properties	161:178	thermal properties	161:178	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	4	19	theme	sorbitol	788:795	arg1	addition					797:804	the sorbitol addition	784:804	the sorbitol addition	784:804	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	0	20	theme	content	19:25	arg1	Effect					0:5	Effect	0:5	Effect of sorbitol content on microstructure and thermal properties of chitosan films.	0:85	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	3	21	theme	sorbitol	709:716	arg1	content					698:704	high content	693:704	high content of sorbitol (~50 wt%)	693:726	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	2	22	theme	chitosan	519:526	arg1	films					528:532	chitosan films	519:532	chitosan films	519:532	It showed that the addition of sorbitol weakened the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films.
30114423	6	23	theme	phase	1090:1094	arg1	transition					1062:1071	the glass-rubber transition	1045:1071	the glass-rubber transition of sorbitol-rich phase	1045:1094	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	7	24	theme	chitosan	1239:1246	arg1	films					1248:1252	the plasticized chitosan films	1223:1252	the plasticized chitosan films	1223:1252	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	0	25	theme	sorbitol	10:17	arg1	content					19:25	sorbitol content	10:25	sorbitol content	10:25	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	1	26	theme	X-ray	266:270	arg1	XRD					285:287	XRD	285:287	XRD	285:287	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	26	theme	X-ray	266:270	arg1	diffraction					272:282	X-ray diffraction	266:282	X-ray diffraction (XRD)	266:288	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	4	27	theme	films	854:858	arg1	stability					833:841	the thermal stability	821:841	the thermal stability of polymer films	821:858	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	1	28	dep	Fourier	218:224	arg1	transform					226:234	transform	226:234	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC)	226:366	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	6	29	theme	sorbitol-rich	1076:1088	arg1	phase					1090:1094	sorbitol-rich phase	1076:1094	sorbitol-rich phase	1076:1094	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	1	30	theme	chitosan	183:190	arg1	films					192:196	chitosan films	183:196	chitosan films	183:196	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	31	dep	transform	226:234	arg1	infrared					236:243	infrared	236:243	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC)	226:366	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	4	32	theme	composite	753:761	arg1	films					763:767	composite films	753:767	composite films	753:767	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	1	33	theme	films	192:196	arg1	microstructure					142:155	microstructure	142:155	microstructure	142:155	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	33	theme	films	192:196	arg1	properties					169:178	thermal properties	161:178	thermal properties	161:178	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	2	34	theme	hydrogen	422:429	arg1	bonding					431:437	the hydrogen bonding	418:437	the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films	418:532	It showed that the addition of sorbitol weakened the hydrogen bonding between chitosan molecules as well as the electrostatic interactions existed in chitosan films.
30114423	3	35	theme	small	571:575	arg1	sorbitol					587:594	sorbitol	587:594	sorbitol (~10 wt%)	587:604	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	3	35	theme	small	571:575	arg1	%					603:603	~10 wt%	597:603	~10 wt%	597:603	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	3	35	theme	small	571:575	arg1	amount					577:582	a small amount	569:582	a small amount of sorbitol (~10 wt%)	569:604	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	7	36	theme	plasticized	1227:1237	arg1	films					1248:1252	the plasticized chitosan films	1223:1252	the plasticized chitosan films	1223:1252	These results implied that the microstructure as well as properties of the plasticized chitosan films can be controlled by adding sorbitol, which is important for their usage as packaging materials.
30114423	0	37	theme	thermal	49:55	arg1	properties					57:66	thermal properties	49:66	thermal properties	49:66	Effect of sorbitol content on microstructure and thermal properties of chitosan films.
30114423	1	38	dep	microstructure	142:155	arg1	the					138:140	the	138:140	the	138:140	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	4	39	theme	thermal	825:831	arg1	stability					833:841	the thermal stability	821:841	the thermal stability of polymer films	821:858	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	4	40	theme	films	763:767	arg1	behavior					741:748	The thermal behavior	729:748	The thermal behavior of composite films	729:767	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	4	41	theme	polymer	846:852	arg1	films					854:858	polymer films	846:858	polymer films	846:858	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	5	42	theme	phase	909:913	arg1	separation					915:924	phase separation	909:924	phase separation	909:924	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	3	43	theme	sorbitol	587:594	arg1	sorbitol					587:594	sorbitol	587:594	sorbitol (~10 wt%)	587:604	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	3	43	theme	sorbitol	587:594	arg1	%					603:603	~10 wt%	597:603	~10 wt%	597:603	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	3	43	theme	sorbitol	587:594	arg1	amount					577:582	a small amount	569:582	a small amount of sorbitol (~10 wt%)	569:604	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	3	44	theme	high	693:696	arg1	content					698:704	high content	693:704	high content of sorbitol (~50 wt%)	693:726	Structural analysis revealed that a small amount of sorbitol (~10 wt%) could facilitate the crystallization of chitosan, whereas it was seriously hindered at high content of sorbitol (~50 wt%).
30114423	5	45	theme	composite	975:983	arg1	films					985:989	composite films	975:989	composite films	975:989	When the sorbitol content rose to above 30 wt%, phase separation may occur and two glass transitions presented in composite films.
30114423	6	46	theme	chitosan-rich	1131:1143	arg1	phase					1145:1149	chitosan-rich phase	1131:1149	chitosan-rich phase	1131:1149	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	6	47	theme	glass-rubber	1049:1060	arg1	transition					1062:1071	the glass-rubber transition	1045:1071	the glass-rubber transition of sorbitol-rich phase	1045:1094	The lower temperature transition was associated with the glass-rubber transition of sorbitol-rich phase, and the higher one was related to chitosan-rich phase.
30114423	1	48	theme	sorbitol	118:125	arg1	content					127:133	sorbitol content	118:133	sorbitol content	118:133	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	49	theme	Thermogravimetric	291:307	arg1	TGA					319:321	TGA	319:321	TGA	319:321	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	1	49	theme	Thermogravimetric	291:307	arg1	analysis					309:316	Thermogravimetric analysis	291:316	Thermogravimetric analysis (TGA)	291:322	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
30114423	4	50	theme	thermal	733:739	arg1	behavior					741:748	The thermal behavior	729:748	The thermal behavior of composite films	729:767	The thermal behavior of composite films indicated that the sorbitol addition did not reduce the thermal stability of polymer films.
30114423	1	51	theme	content	127:133	arg1	influence					105:113	the influence	101:113	the influence of sorbitol content on the microstructure and thermal properties of chitosan films	101:196	In this work, the influence of sorbitol content on the microstructure and thermal properties of chitosan films was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC).
31108781	10	0	from	sugars	954:959	arg1	rich					939:942	rich	939:942	rich	939:942	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	17	1	from	industry	2124:2131	arg1	useful					2105:2110	useful	2105:2110	useful	2105:2110	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	11	2	theme	synthesis	1044:1052	arg1	FOS					1059:1061	FOS	1059:1061	FOS	1059:1061	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	11	2	theme	synthesis	1044:1052	arg1	products					1032:1039	The main products	1023:1039	The main products of synthesis	1023:1052	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	17	3	theme	lactic	1931:1936	arg1	bacteria					1943:1950	lactic acid bacteria	1931:1950	lactic acid bacteria	1931:1950	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	11	4	with	comparable	1138:1147	arg1	those					1154:1158	those	1154:1158	those	1154:1158	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	2	5	used	used	233:236	arg2	crop					223:226	a sucrose-rich crop	208:226	a sucrose-rich crop	208:226	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	2	5	used	used	233:236	arg2	nigra					201:205	Prosopis nigra	192:205	Prosopis nigra	192:205	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	17	6	theme	bacteria	1943:1950	arg1	presence					1869:1876	The concomitant presence	1853:1876	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices	1853:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	7	from	useful	2105:2110	arg1	industry					2124:2131	the food industry	2115:2131	the food industry	2115:2131	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	8	from	presence	1869:1876	arg1	matrices					1959:1966	the matrices	1955:1966	the matrices	1955:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	0	9	theme	dehydrated	136:145	arg1	subsp					173:177	dehydrated Lactobacillus delbrueckii subsp	136:177	dehydrated Lactobacillus delbrueckii subsp	136:177	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	10	10	theme	dietary	991:997	arg1	fiber					999:1003	total dietary fiber	985:1003	total dietary fiber	985:1003	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	12	11	dep	obtained	1203:1210	arg1	product					1195:1201	the first product	1185:1201	the first product	1185:1201	DP3 was the first product obtained, attaining the maximal production after 1.29 hours of synthesis.
31108781	17	12	theme	prebiotics	1881:1890	arg1	presence					1869:1876	The concomitant presence	1853:1876	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices	1853:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	13	13	theme	total	1303:1307	arg1	DP3 + DP4 + DP5					1314:1328	DP3 + DP4 + DP5	1314:1328	DP3 + DP4 + DP5	1314:1328	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	13	13	theme	total	1303:1307	arg1	FOS					1309:1311	total FOS	1303:1311	total FOS (DP3 + DP4 + DP5)	1303:1329	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	0	14	theme	delbrueckii	161:171	arg1	subsp					173:177	dehydrated Lactobacillus delbrueckii subsp	136:177	dehydrated Lactobacillus delbrueckii subsp	136:177	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	16	15	theme	CIDCA	1693:1697	arg1	strain					1703:1708	bulgaricus CIDCA 333 strain	1682:1708	bulgaricus CIDCA 333 strain during freeze-drying and storage for up	1682:1748	bulgaricus CIDCA 333 strain during freeze-drying and storage for up to 140 days at 4 °C, and were significantly better protectants than the controls of sucrose (p <0.05).
31108781	7	16	theme	pure	705:708	arg1	sucrose					710:716	pure sucrose	705:716	pure sucrose	705:716	The progress of the enzymatic reaction was monitored by HPLC and compared with a reaction carried out using equivalent concentrations of pure sucrose as substrate (control).
31108781	10	17	from	rich	939:942	arg1	fiber					999:1003	total dietary fiber	985:1003	total dietary fiber	985:1003	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	17	from	rich	939:942	arg1	polyphenols					1010:1020	polyphenols	1010:1020	polyphenols	1010:1020	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	17	from	rich	939:942	arg1	sucrose					962:968	sucrose	962:968	sucrose	962:968	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	17	from	rich	939:942	arg1	sugars					954:959	simple sugars	947:959	simple sugars (sucrose and fructose)	947:982	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	17	from	rich	939:942	arg1	fructose					974:981	fructose	974:981	fructose	974:981	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	13	18	dep	FOS	1385:1387	arg1	DP4					1390:1392	DP4	1390:1392	DP4	1390:1392	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	13	18	dep	FOS	1385:1387	arg1	DP5					1395:1397	DP5	1395:1397	DP5	1395:1397	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	15	19	theme	nigra	1613:1617	arg1	matrices					1619:1626	Both P. nigra + FOS or P. nigra matrices	1587:1626	Both P. nigra + FOS or P. nigra matrices	1587:1626	Both P. nigra + FOS or P. nigra matrices stabilized the highly sensitive L. delbrueckii subsp.
31108781	3	20	theme	stabilizing	332:342	arg1	matrices					344:351	stabilizing matrices	332:351	stabilizing matrices	332:351	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	3	20	theme	stabilizing	332:342	arg1	products					310:317	The obtained products	297:317	The obtained products	297:317	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	10	21	from	fiber	999:1003	arg1	rich					939:942	rich	939:942	rich	939:942	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	22	theme	P.	920:921	arg1	flour					929:933	P. nigra flour	920:933	P. nigra flour	920:933	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	17	23	theme	useful	2105:2110	arg1	ingredient					2082:2091	an interesting ingredient	2067:2091	an interesting ingredient potentially useful in the food industry	2067:2131	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	12	24	theme	first	1189:1193	arg1	product					1195:1201	the first product	1185:1201	the first product	1185:1201	DP3 was the first product obtained, attaining the maximal production after 1.29 hours of synthesis.
31108781	17	25	theme	underutilized	2024:2036	arg1	crop					2047:2050	this underutilized regional crop	2019:2050	this underutilized regional crop	2019:2050	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	0	26	theme	fructo-oligosaccharides	91:113	arg1	synthesis					68:76	the synthesis	64:76	the synthesis of prebiotic fructo-oligosaccharides	64:113	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	8	27	theme	delbrueckii	879:889	arg1	subsp					891:895	L. delbrueckii subsp	876:895	L. delbrueckii subsp	876:895	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	0	28	from	nigra	27:31	arg1	Flour					0:4	Flour	0:4	Flour from mature Prosopis nigra	0:31	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	17	29	theme	crop	2047:2050	arg1	value					2010:2014	the value	2006:2014	the value of this underutilized regional crop	2006:2050	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	12	30	theme	maximal	1227:1233	arg1	production					1235:1244	the maximal production	1223:1244	the maximal production	1223:1244	DP3 was the first product obtained, attaining the maximal production after 1.29 hours of synthesis.
31108781	3	31	theme	delbrueckii	403:413	arg1	subsp					415:419	Lactobacillus delbrueckii subsp	389:419	Lactobacillus delbrueckii subsp	389:419	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	15	32	theme	L.	1660:1661	arg1	subsp					1675:1679	the highly sensitive L. delbrueckii subsp	1639:1679	the highly sensitive L. delbrueckii subsp	1639:1679	Both P. nigra + FOS or P. nigra matrices stabilized the highly sensitive L. delbrueckii subsp.
31108781	0	33	theme	mature	11:16	arg1	nigra					27:31	mature Prosopis nigra	11:31	mature Prosopis nigra	11:31	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	11	34	with	FOS	1059:1061	arg1	degrees					1068:1074	degrees	1068:1074	degrees of polymerization (DP)	1068:1097	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	16	35	dep	140 days	1753:1760	arg1	to					1750:1751	to	1750:1751	to	1750:1751	bulgaricus CIDCA 333 strain during freeze-drying and storage for up to 140 days at 4 °C, and were significantly better protectants than the controls of sucrose (p <0.05).
31108781	7	36	theme	enzymatic	588:596	arg1	reaction					598:605	the enzymatic reaction	584:605	the enzymatic reaction	584:605	The progress of the enzymatic reaction was monitored by HPLC and compared with a reaction carried out using equivalent concentrations of pure sucrose as substrate (control).
31108781	17	37	theme	smart	1979:1983	arg1	strategy					1985:1992	a smart strategy	1977:1992	a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry	1977:2131	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	16	38	theme	bulgaricus	1682:1691	arg1	strain					1703:1708	bulgaricus CIDCA 333 strain	1682:1708	bulgaricus CIDCA 333 strain during freeze-drying and storage for up	1682:1748	bulgaricus CIDCA 333 strain during freeze-drying and storage for up to 140 days at 4 °C, and were significantly better protectants than the controls of sucrose (p <0.05).
31108781	11	39	theme	polymerization	1079:1092	arg1	degrees					1068:1074	degrees	1068:1074	degrees of polymerization (DP)	1068:1097	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	8	40	contain	containing	757:766	arg1	nigra					751:755	P. nigra	748:755	P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra)	748:819	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	8	40	contain	containing	757:766	arg1	matrices					834:841	matrices	834:841	matrices for freeze-drying and storage of L. delbrueckii subsp	834:895	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	8	40	contain	containing	757:766	arg2	FOS					788:790	FOS	788:790	FOS (P. nigra + FOS or P. nigra)	788:819	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	5	41	theme	flour	483:487	arg1	composition					459:469	The centesimal composition	444:469	The centesimal composition of P. nigra flour	444:487	The centesimal composition of P. nigra flour was firstly determined.
31108781	14	42	dep	Vmax	1496:1499	arg1	velocity					1517:1524	maximal velocity	1509:1524	maximal velocity for the production and constant for the formation	1509:1574	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	5	43	theme	P.	474:475	arg1	flour					483:487	P. nigra flour	474:487	P. nigra flour	474:487	The centesimal composition of P. nigra flour was firstly determined.
31108781	17	44	theme	concomitant	1857:1867	arg1	presence					1869:1876	The concomitant presence	1853:1876	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices	1853:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	8	45	dep	FOS	788:790	arg1	nigra + FOS					796:806	P. nigra + FOS	793:806	P. nigra + FOS	793:806	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	8	45	dep	FOS	788:790	arg1	nigra					814:818	P. nigra	811:818	P. nigra	811:818	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	14	46	dep	production	1534:1543	arg1	the					1530:1532	the	1530:1532	the	1530:1532	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	11	47	theme	main	1027:1030	arg1	FOS					1059:1061	FOS	1059:1061	FOS	1059:1061	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	11	47	theme	main	1027:1030	arg1	products					1032:1039	The main products	1023:1039	The main products of synthesis	1023:1052	The main products of synthesis were FOS with degrees of polymerization (DP) within 3 and 5, and these results were comparable with those of the controls.
31108781	17	48	theme	acid	1938:1941	arg1	bacteria					1943:1950	lactic acid bacteria	1931:1950	lactic acid bacteria	1931:1950	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	10	49	theme	simple	947:952	arg1	fructose					974:981	fructose	974:981	fructose	974:981	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	49	theme	simple	947:952	arg1	sucrose					962:968	sucrose	962:968	sucrose	962:968	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	49	theme	simple	947:952	arg1	sugars					954:959	simple sugars	947:959	simple sugars (sucrose and fructose)	947:982	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	3	50	theme	obtained	301:308	arg1	matrices					344:351	stabilizing matrices	332:351	stabilizing matrices	332:351	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	3	50	theme	obtained	301:308	arg1	products					310:317	The obtained products	297:317	The obtained products	297:317	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	17	51	theme	food	2119:2122	arg1	industry					2124:2131	the food industry	2115:2131	the food industry	2115:2131	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	0	52	theme	Lactobacillus	147:159	arg1	subsp					173:177	dehydrated Lactobacillus delbrueckii subsp	136:177	dehydrated Lactobacillus delbrueckii subsp	136:177	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	12	53	theme	synthesis	1266:1274	arg1	hours					1257:1261	1.29 hours	1252:1261	1.29 hours of synthesis	1252:1274	DP3 was the first product obtained, attaining the maximal production after 1.29 hours of synthesis.
31108781	8	54	used	used	826:829	arg2	nigra					751:755	P. nigra	748:755	P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra)	748:819	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	8	54	used	used	826:829	arg2	matrices					834:841	matrices	834:841	matrices for freeze-drying and storage of L. delbrueckii subsp	834:895	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	10	55	from	polyphenols	1010:1020	arg1	rich					939:942	rich	939:942	rich	939:942	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	0	56	theme	subsp	173:177	arg1	substrate					50:58	suitable substrate	41:58	suitable substrate for the synthesis of prebiotic fructo-oligosaccharides	41:113	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	0	56	theme	subsp	173:177	arg1	stabilization					119:131	stabilization	119:131	stabilization of dehydrated Lactobacillus delbrueckii subsp	119:177	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	10	57	theme	total	985:989	arg1	fiber					999:1003	total dietary fiber	985:1003	total dietary fiber	985:1003	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	16	58	theme	333	1699:1701	arg1	strain					1703:1708	bulgaricus CIDCA 333 strain	1682:1708	bulgaricus CIDCA 333 strain during freeze-drying and storage for up	1682:1748	bulgaricus CIDCA 333 strain during freeze-drying and storage for up to 140 days at 4 °C, and were significantly better protectants than the controls of sucrose (p <0.05).
31108781	3	59	used	used	324:327	arg2	products					310:317	The obtained products	297:317	The obtained products	297:317	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	3	59	used	used	324:327	arg2	matrices					344:351	stabilizing matrices	332:351	stabilizing matrices	332:351	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	14	60	theme	lower	1490:1494	arg1	Vmax					1496:1499	significantly lower Vmax	1476:1499	significantly lower Vmax	1476:1499	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	13	61	theme	FOS	1309:1311	arg1	production					1289:1298	The maximal production	1277:1298	The maximal production of total FOS (DP3 + DP4 + DP5)	1277:1329	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	8	62	theme	obtained	779:786	arg1	FOS					788:790	FOS	788:790	FOS (P. nigra + FOS or P. nigra)	788:819	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	7	63	theme	sucrose	710:716	arg1	concentrations					687:700	equivalent concentrations	676:700	equivalent concentrations of pure sucrose	676:716	The progress of the enzymatic reaction was monitored by HPLC and compared with a reaction carried out using equivalent concentrations of pure sucrose as substrate (control).
31108781	17	64	theme	antioxidants	1899:1910	arg1	presence					1869:1876	The concomitant presence	1853:1876	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices	1853:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	15	65	theme	nigra + FOS	1595:1605	arg1	matrices					1619:1626	Both P. nigra + FOS or P. nigra matrices	1587:1626	Both P. nigra + FOS or P. nigra matrices	1587:1626	Both P. nigra + FOS or P. nigra matrices stabilized the highly sensitive L. delbrueckii subsp.
31108781	13	66	theme	maximal	1281:1287	arg1	production					1289:1298	The maximal production	1277:1298	The maximal production of total FOS (DP3 + DP4 + DP5)	1277:1329	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	17	67	attach	presence	1869:1876	arg2	antioxidants					1899:1910	antioxidants	1899:1910	antioxidants (polypyhenols)	1899:1925	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	67	attach	presence	1869:1876	arg1	matrices					1959:1966	the matrices	1955:1966	the matrices	1955:1966	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	67	attach	presence	1869:1876	arg2	polypyhenols					1913:1924	polypyhenols	1913:1924	polypyhenols	1913:1924	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	67	attach	presence	1869:1876	arg2	bacteria					1943:1950	lactic acid bacteria	1931:1950	lactic acid bacteria	1931:1950	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	67	attach	presence	1869:1876	arg2	prebiotics					1881:1890	prebiotics	1881:1890	prebiotics (FOS)	1881:1896	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	67	attach	presence	1869:1876	arg2	FOS					1893:1895	FOS	1893:1895	FOS	1893:1895	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	17	68	theme	interesting	2070:2080	arg1	ingredient					2082:2091	an interesting ingredient	2067:2091	an interesting ingredient potentially useful in the food industry	2067:2131	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	14	69	theme	secondary	1448:1456	arg1	product					1458:1464	secondary product	1448:1464	secondary product	1448:1464	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	14	69	theme	secondary	1448:1456	arg1	Glucose					1424:1430	Glucose	1424:1430	Glucose	1424:1430	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	0	70	theme	prebiotic	81:89	arg1	fructo-oligosaccharides					91:113	prebiotic fructo-oligosaccharides	81:113	prebiotic fructo-oligosaccharides	81:113	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	7	71	theme	equivalent	676:685	arg1	concentrations					687:700	equivalent concentrations	676:700	equivalent concentrations of pure sucrose	676:716	The progress of the enzymatic reaction was monitored by HPLC and compared with a reaction carried out using equivalent concentrations of pure sucrose as substrate (control).
31108781	10	72	theme	nigra	923:927	arg1	flour					929:933	P. nigra flour	920:933	P. nigra flour	920:933	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	3	73	theme	Lactobacillus	389:401	arg1	subsp					415:419	Lactobacillus delbrueckii subsp	389:419	Lactobacillus delbrueckii subsp	389:419	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	14	74	theme	maximal	1509:1515	arg1	velocity					1517:1524	maximal velocity	1509:1524	maximal velocity for the production and constant for the formation	1509:1574	Glucose was obtained as secondary product, but with significantly lower Vmax and Kf (maximal velocity for the production and constant for the formation) than DP3.
31108781	17	75	theme	regional	2038:2045	arg1	crop					2047:2050	this underutilized regional crop	2019:2050	this underutilized regional crop	2019:2050	The concomitant presence of prebiotics (FOS), antioxidants (polypyhenols) and lactic acid bacteria in the matrices provides a smart strategy to increase the value of this underutilized regional crop, turning it in an interesting ingredient potentially useful in the food industry.
31108781	15	76	theme	delbrueckii	1663:1673	arg1	subsp					1675:1679	the highly sensitive L. delbrueckii subsp	1639:1679	the highly sensitive L. delbrueckii subsp	1639:1679	Both P. nigra + FOS or P. nigra matrices stabilized the highly sensitive L. delbrueckii subsp.
31108781	8	77	theme	L.	876:877	arg1	subsp					891:895	L. delbrueckii subsp	876:895	L. delbrueckii subsp	876:895	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	15	78	theme	sensitive	1650:1658	arg1	subsp					1675:1679	the highly sensitive L. delbrueckii subsp	1639:1679	the highly sensitive L. delbrueckii subsp	1639:1679	Both P. nigra + FOS or P. nigra matrices stabilized the highly sensitive L. delbrueckii subsp.
31108781	0	79	theme	Prosopis	18:25	arg1	nigra					27:31	mature Prosopis nigra	11:31	mature Prosopis nigra	11:31	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	7	80	theme	reaction	598:605	arg1	progress					572:579	The progress	568:579	The progress of the enzymatic reaction	568:605	The progress of the enzymatic reaction was monitored by HPLC and compared with a reaction carried out using equivalent concentrations of pure sucrose as substrate (control).
31108781	8	81	theme	subsp	891:895	arg1	storage					865:871	storage	865:871	storage	865:871	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	8	81	theme	subsp	891:895	arg1	freeze-drying					847:859	freeze-drying	847:859	freeze-drying	847:859	Then, P. nigra containing or not the obtained FOS (P. nigra + FOS or P. nigra) were used as matrices for freeze-drying and storage of L. delbrueckii subsp.
31108781	10	82	dep	sugars	954:959	arg1	fructose					974:981	fructose	974:981	fructose	974:981	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	82	dep	sugars	954:959	arg1	sucrose					962:968	sucrose	962:968	sucrose	962:968	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	10	82	dep	sugars	954:959	arg1	sugars					954:959	simple sugars	947:959	simple sugars (sucrose and fructose)	947:982	P. nigra flour was rich in simple sugars (sucrose and fructose), total dietary fiber, and polyphenols.
31108781	3	83	theme	subsp	415:419	arg1	storage					378:384	storage	378:384	storage	378:384	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	3	83	theme	subsp	415:419	arg1	freeze-drying					360:372	freeze-drying	360:372	freeze-drying	360:372	The obtained products were used as stabilizing matrices during freeze-drying and storage of Lactobacillus delbrueckii subsp.
31108781	6	84	theme	Viscozyme	540:548	arg1	L					550:550	Viscozyme L	540:550	Viscozyme L	540:550	FOS were synthesized using Viscozyme L as biocatalyst.
31108781	2	85	theme	Prosopis	192:199	arg1	nigra					201:205	Prosopis nigra	192:205	Prosopis nigra	192:205	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	2	85	theme	Prosopis	192:199	arg1	crop					223:226	a sucrose-rich crop	208:226	a sucrose-rich crop	208:226	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	0	86	theme	suitable	41:48	arg1	substrate					50:58	suitable substrate	41:58	suitable substrate for the synthesis of prebiotic fructo-oligosaccharides	41:113	Flour from mature Prosopis nigra pods as suitable substrate for the synthesis of prebiotic fructo-oligosaccharides and stabilization of dehydrated Lactobacillus delbrueckii subsp.
31108781	5	87	theme	nigra	477:481	arg1	flour					483:487	P. nigra flour	474:487	P. nigra flour	474:487	The centesimal composition of P. nigra flour was firstly determined.
31108781	2	88	theme	sucrose-rich	210:221	arg1	nigra					201:205	Prosopis nigra	192:205	Prosopis nigra	192:205	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	2	88	theme	sucrose-rich	210:221	arg1	crop					223:226	a sucrose-rich crop	208:226	a sucrose-rich crop	208:226	Prosopis nigra, a sucrose-rich crop, was used to enzymatically synthesize fructo-oligosaccharides (FOS).
31108781	13	89	theme	larger	1378:1383	arg1	FOS					1385:1387	larger FOS	1378:1387	larger FOS (DP4, DP5)	1378:1398	The maximal production of total FOS (DP3 + DP4 + DP5) was achieved after 2.57 hours, indicating that larger FOS (DP4, DP5) were produced from DP3.
31108781	16	90	theme	sucrose	1834:1840	arg1	controls					1822:1829	the controls	1818:1829	the controls of sucrose (p <0.05)	1818:1850	bulgaricus CIDCA 333 strain during freeze-drying and storage for up to 140 days at 4 °C, and were significantly better protectants than the controls of sucrose (p <0.05).
31108781	5	91	theme	centesimal	448:457	arg1	composition					459:469	The centesimal composition	444:469	The centesimal composition of P. nigra flour	444:487	The centesimal composition of P. nigra flour was firstly determined.
31207008	8	0	theme	efficacy	1269:1276	arg1	report					1255:1260	the first report	1245:1260	the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation	1245:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	8	0	theme	efficacy	1269:1276	arg1	This					1237:1240	This	1237:1240	This	1237:1240	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	5	1	theme	produced	840:847	arg1	mAb					849:851	WT produced mAb	837:851	WT produced mAb	837:851	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	1	2	theme	debilitating	252:263	arg1	arthritis					265:273	long-term debilitating arthritis	242:273	long-term debilitating arthritis in humans	242:283	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	0	3	theme	antibodies	67:76	arg1	efficacy					21:28	In vitro and in vivo efficacy	0:28	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies	0:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	8	4	theme	in	1465:1466	arg1	activity					1473:1480	in vivo activity	1465:1480	in vivo activity	1465:1480	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	7	5	dep	in	1142:1143	arg1	vivo					1145:1148	vivo	1145:1148	vivo	1145:1148	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	6	6	theme	neutralization	1067:1080	arg1	activity					1082:1089	potent in vitro neutralization activity	1051:1089	potent in vitro neutralization activity against CHIKV	1051:1103	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	3	7	theme	wild-type	490:498	arg1	plants					554:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	0	8	theme	wild-type	90:98	arg1	plants					142:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	3	9	theme	glycoengineered	509:523	arg1	plants					554:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	5	10	theme	typical	900:906	arg1	glycans					922:928	the typical plant GnGnXF3 glycans	896:928	the typical plant GnGnXF3 glycans	896:928	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	11	theme	N-glycans	876:884	arg1	mixture					865:871	a mixture	863:871	a mixture of N-glycans including the typical plant GnGnXF3 glycans	863:928	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	8	12	theme	activity	1473:1480	arg1	optimization					1449:1460	optimization	1449:1460	optimization of in vivo activity by controlling Fc glycosylation	1449:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	5	13	theme	GnGnXF3	914:920	arg1	glycans					922:928	the typical plant GnGnXF3 glycans	896:928	the typical plant GnGnXF3 glycans	896:928	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	4	14	dep	plant	660:664	arg1	leaves					666:671	leaves	666:671	leaves	666:671	CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme.
31207008	8	15	theme	first	1249:1253	arg1	report					1255:1260	the first report	1245:1260	the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation	1245:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	8	15	theme	first	1249:1253	arg1	This					1237:1240	This	1237:1240	This	1237:1240	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	0	16	theme	glycoengineered	104:118	arg1	plants					142:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	7	17	theme	in	1142:1143	arg1	efficacy					1150:1157	in vivo efficacy	1142:1157	in vivo efficacy	1142:1157	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	8	18	theme	CHIKV	1426:1430	arg1	mAbs					1432:1435	functionally active CHIKV mAbs	1406:1435	functionally active CHIKV mAbs	1406:1435	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	6	19	theme	potent	1051:1056	arg1	activity					1082:1089	potent in vitro neutralization activity	1051:1089	potent in vitro neutralization activity against CHIKV	1051:1103	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	8	20	dep	in	1465:1466	arg1	vivo					1468:1471	vivo	1468:1471	vivo	1468:1471	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	5	21	theme	single	771:776	arg1	structures					825:834	GnGn structures	820:834	namely GnGn structures	813:834	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	21	theme	single	771:776	arg1	species					787:793	a single N-glycan species	769:793	a single N-glycan species at the Fc domain	769:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	6	22	theme	in	1058:1059	arg1	activity					1082:1089	potent in vitro neutralization activity	1051:1089	potent in vitro neutralization activity against CHIKV	1051:1103	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	3	23	theme	Nicotiana	532:540	arg1	plants					554:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	5	24	theme	GnGn	820:823	arg1	species					787:793	a single N-glycan species	769:793	a single N-glycan species at the Fc domain	769:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	24	theme	GnGn	820:823	arg1	structures					825:834	GnGn structures	820:834	namely GnGn structures	813:834	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	0	25	theme	In	0:1	arg1	efficacy					21:28	In vitro and in vivo efficacy	0:28	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies	0:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	8	26	theme	mAbs	1432:1435	arg1	production					1392:1401	production	1392:1401	production of functionally active CHIKV mAbs	1392:1435	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	2	27	theme	licensed	310:317	arg1	vaccines					319:326	no licensed vaccines	307:326	no licensed vaccines	307:326	Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections.
31207008	1	28	theme	mosquito-transmitted	181:200	arg1	virus					162:166	Chikungunya virus	150:166	Chikungunya virus (CHIKV)	150:174	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	1	28	theme	mosquito-transmitted	181:200	arg1	alphavirus					202:211	a mosquito-transmitted alphavirus	179:211	a mosquito-transmitted alphavirus	179:211	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	0	29	theme	in	13:14	arg1	efficacy					21:28	In vitro and in vivo efficacy	0:28	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies	0:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	8	30	theme	Fc	1497:1498	arg1	glycosylation					1500:1512	Fc glycosylation	1497:1512	Fc glycosylation	1497:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	0	31	theme	benthamiana	130:140	arg1	plants					142:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	4	32	theme	purification	713:724	arg1	scheme					726:731	a simple purification scheme	704:731	a simple purification scheme	704:731	CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme.
31207008	3	33	theme	monoclonal	452:461	arg1	mAb					473:475	mAb	473:475	mAb	473:475	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	3	33	theme	monoclonal	452:461	arg1	antibody					463:470	an anti-CHIKV monoclonal antibody	438:470	an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model	438:604	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	3	34	theme	benthamiana	542:552	arg1	plants					554:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants	490:559	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	0	35	theme	Nicotiana	120:128	arg1	plants					142:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	wild-type and glycoengineered Nicotiana benthamiana plants	90:147	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	4	36	theme	simple	706:711	arg1	scheme					726:731	a simple purification scheme	704:731	a simple purification scheme	704:731	CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme.
31207008	1	37	theme	Chikungunya	150:160	arg1	virus					162:166	Chikungunya virus	150:166	Chikungunya virus (CHIKV)	150:174	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	1	37	theme	Chikungunya	150:160	arg1	alphavirus					202:211	a mosquito-transmitted alphavirus	179:211	a mosquito-transmitted alphavirus	179:211	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	1	37	theme	Chikungunya	150:160	arg1	CHIKV					169:173	CHIKV	169:173	CHIKV	169:173	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	7	38	theme	mAb	1120:1122	arg1	glycoforms					1124:1133	both mAb glycoforms	1115:1133	both mAb glycoforms	1115:1133	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	5	39	theme	plant	908:912	arg1	glycans					922:928	the typical plant GnGnXF3 glycans	896:928	the typical plant GnGnXF3 glycans	896:928	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	6	40	theme	WT	1006:1007	arg1	mAbs					1033:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	7	41	gly	glycoforms	1124:1133	arg1	mAb					1120:1122	both mAb glycoforms	1115:1133	both mAb glycoforms	1115:1133	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	6	42	theme	plant-produced	1018:1031	arg1	mAbs					1033:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	5	43	theme	Fc	802:803	arg1	domain					805:810	the Fc domain	798:810	the Fc domain	798:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	1	44	from	arthritis	265:273	arg1	humans					278:283	humans	278:283	humans	278:283	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
31207008	3	45	theme	CHIKV	573:577	arg1	infection					579:587	CHIKV infection	573:587	CHIKV infection	573:587	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	3	46	theme	anti-CHIKV	441:450	arg1	mAb					473:475	mAb	473:475	mAb	473:475	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	3	46	theme	anti-CHIKV	441:450	arg1	antibody					463:470	an anti-CHIKV monoclonal antibody	438:470	an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model	438:604	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	0	47	theme	anti-chikungunya	33:48	arg1	antibodies					67:76	anti-chikungunya virus monoclonal antibodies	33:76	anti-chikungunya virus monoclonal antibodies	33:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	7	48	theme	mouse	1164:1168	arg1	model					1170:1174	a mouse model	1162:1174	a mouse model	1162:1174	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	8	49	theme	mAbs	1296:1299	arg1	efficacy					1269:1276	the efficacy	1265:1276	the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation	1265:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	7	50	theme	slight	1184:1189	arg1	efficacy					1201:1208	a slight increased efficacy	1182:1208	a slight increased efficacy by the ∆XFT-produced mAbs	1182:1234	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	5	51	from	domain	805:810	arg1	structures					825:834	GnGn structures	820:834	namely GnGn structures	813:834	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	51	from	domain	805:810	arg1	species					787:793	a single N-glycan species	769:793	a single N-glycan species at the Fc domain	769:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	2	52	theme	human	348:352	arg1	use					354:356	human use	348:356	human use to combat CHIKV infections	348:383	Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections.
31207008	8	53	theme	effective	1369:1377	arg1	plants					1356:1361	plants	1356:1361	plants	1356:1361	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	8	53	theme	effective	1369:1377	arg1	platform					1379:1386	an effective platform	1366:1386	an effective platform for production of functionally active CHIKV mAbs	1366:1435	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	5	54	contain	carried	761:767	arg2	species					787:793	a single N-glycan species	769:793	a single N-glycan species at the Fc domain	769:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	54	contain	carried	761:767	arg2	structures					825:834	GnGn structures	820:834	namely GnGn structures	813:834	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	54	contain	carried	761:767	arg1	mAb					740:742	mAb	740:742	mAb produced in ∆XFT	740:759	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	55	theme	N-glycan	778:785	arg1	structures					825:834	GnGn structures	820:834	namely GnGn structures	813:834	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	5	55	theme	N-glycan	778:785	arg1	species					787:793	a single N-glycan species	769:793	a single N-glycan species at the Fc domain	769:810	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	3	56	dep	wild-type	490:498	arg1	WT					501:502	WT	501:502	WT	501:502	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	5	57	theme	processed	959:967	arg1	structures					989:998	incompletely processed and oligomannosidic structures	946:998	incompletely processed and oligomannosidic structures	946:998	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	8	58	theme	active	1419:1424	arg1	mAbs					1432:1435	functionally active CHIKV mAbs	1406:1435	functionally active CHIKV mAbs	1406:1435	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	0	59	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	5	60	theme	oligomannosidic	973:987	arg1	structures					989:998	incompletely processed and oligomannosidic structures	946:998	incompletely processed and oligomannosidic structures	946:998	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	3	61	dep	glycoengineered	509:523	arg1	∆XFT					526:529	∆XFT	526:529	∆XFT	526:529	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	3	62	theme	mouse	594:598	arg1	model					600:604	a mouse model	592:604	a mouse model	592:604	In this study, we explored the feasibility of using an anti-CHIKV monoclonal antibody (mAb) produced in wild-type (WT) and glycoengineered (∆XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model.
31207008	5	63	theme	WT	837:838	arg1	mAb					849:851	WT produced mAb	837:851	WT produced mAb	837:851	While mAb produced in ∆XFT carried a single N-glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N-glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures.
31207008	8	64	theme	plant-produced	1281:1294	arg1	mAbs					1296:1299	plant-produced mAbs	1281:1299	plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation	1281:1512	This is the first report of the efficacy of plant-produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation.
31207008	7	65	theme	∆XFT-produced	1217:1229	arg1	mAbs					1231:1234	the ∆XFT-produced mAbs	1213:1234	the ∆XFT-produced mAbs	1213:1234	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	0	66	theme	monoclonal	56:65	arg1	antibodies					67:76	anti-chikungunya virus monoclonal antibodies	33:76	anti-chikungunya virus monoclonal antibodies	33:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	4	67	theme	CHIKV	607:611	arg1	mAb					613:615	CHIKV mAb	607:615	CHIKV mAb	607:615	CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme.
31207008	6	68	theme	∆XFT	1013:1016	arg1	mAbs					1033:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs	1001:1036	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	2	69	theme	CHIKV	368:372	arg1	infections					374:383	combat CHIKV infections	361:383	combat CHIKV infections	361:383	Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections.
31207008	6	70	dep	in	1058:1059	arg1	vitro					1061:1065	vitro	1061:1065	vitro	1061:1065	Both WT and ∆XFT plant-produced mAbs demonstrated potent in vitro neutralization activity against CHIKV.
31207008	0	71	theme	virus	50:54	arg1	antibodies					67:76	anti-chikungunya virus monoclonal antibodies	33:76	anti-chikungunya virus monoclonal antibodies	33:76	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	0	72	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants.
31207008	2	73	theme	combat	361:366	arg1	infections					374:383	combat CHIKV infections	361:383	combat CHIKV infections	361:383	Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections.
31207008	7	74	theme	increased	1191:1199	arg1	efficacy					1201:1208	a slight increased efficacy	1182:1208	a slight increased efficacy by the ∆XFT-produced mAbs	1182:1234	Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ∆XFT-produced mAbs.
31207008	1	75	theme	long-term	242:250	arg1	arthritis					265:273	long-term debilitating arthritis	242:273	long-term debilitating arthritis in humans	242:283	Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, and its infection can cause long-term debilitating arthritis in humans.
29898432	3	0	dep	in	664:665	arg1	silico					667:672	silico	667:672	silico	667:672	First, in silico approach was employed to study the scope of any interaction between the CBG and BA.
29898432	2	1	from	-glucans	383:390	arg1	water					528:532	water	528:532	water	528:532	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	5	2	theme	thermogravimetric	999:1015	arg1	TGA					1027:1029	TGA	1027:1029	TGA	1027:1029	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	5	2	theme	thermogravimetric	999:1015	arg1	analysis					1017:1024	thermogravimetric analysis	999:1024	thermogravimetric analysis (TGA)	999:1030	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	2	3	theme	betulinic	584:592	arg1	acid					594:597	betulinic acid	584:597	betulinic acid	584:597	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	4	4	theme	cyclic	768:773	arg1	complexes					797:805	the cyclic glucan-betulinic acid complexes	764:805	the cyclic glucan-betulinic acid complexes	764:805	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	0	5	theme	drug	52:55	arg1	carriers					57:64	potential drug carriers	42:64	potential drug carriers	42:64	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	4	6	theme	glucan-betulinic	775:790	arg1	complexes					797:805	the cyclic glucan-betulinic acid complexes	764:805	the cyclic glucan-betulinic acid complexes	764:805	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	3	7	theme	interaction	722:732	arg1	scope					709:713	the scope	705:713	the scope of any interaction between the CBG and BA	705:755	First, in silico approach was employed to study the scope of any interaction between the CBG and BA.
29898432	0	8	theme	potential	42:50	arg1	carriers					57:64	potential drug carriers	42:64	potential drug carriers	42:64	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	1	9	theme	low	301:303	arg1	solubility					311:320	low water solubility	301:320	low water solubility	301:320	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	0	10	theme	betulinic	155:163	arg1	acid					165:168	betulinic acid	155:168	betulinic acid	155:168	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	1	11	with	drug	252:255	arg1	properties					279:288	varied medicinal properties	262:288	varied medicinal properties	262:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	5	12	theme	encapsulation	1090:1102	arg1	%					1135:1135	9.53%	1131:1135	9.53%	1131:1135	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	5	12	theme	encapsulation	1090:1102	arg1	efficiency					1104:1113	consequently the encapsulation efficiency	1073:1113	consequently the encapsulation efficiency	1073:1113	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	1	13	theme	water	305:309	arg1	solubility					311:320	low water solubility	301:320	low water solubility	301:320	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	5	14	theme	differential	955:966	arg1	DSC					990:992	DSC	990:992	DSC	990:992	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	5	14	theme	differential	955:966	arg1	calorimetry					977:987	differential scanning calorimetry	955:987	differential scanning calorimetry (DSC)	955:993	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	4	15	theme	1:2	851:853	arg1	CBG					863:865	1:2 and 1:3 CBG	851:865	1:2 and 1:3 CBG:BA	851:868	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	4	15	theme	1:2	851:853	arg1	compositions					830:841	three compositions	824:841	three compositions of 1:1	824:848	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	2	16	contain	having	479:484	arg2	structure					495:503	a cavity structure	486:503	a cavity structure	486:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	16	contain	having	479:484	arg1	10324					473:477	Bradyrhizobium japonicum ATCC 10324	443:477	Bradyrhizobium japonicum ATCC 10324 having a cavity structure	443:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	0	17	theme	cyclic	10:15	arg1	β-					17:18	Microbial cyclic β-	0:18	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.	0:169	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	17	theme	cyclic	10:15	arg1	1→6					26:28	1→6	26:28	1→6	26:28	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	17	theme	cyclic	10:15	arg1	1→3					20:22	1→3	20:22	1→3	20:22	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	3	18	theme	in	664:665	arg1	approach					674:681	in silico approach	664:681	in silico approach	664:681	First, in silico approach was employed to study the scope of any interaction between the CBG and BA.
29898432	5	19	theme	computational	1047:1059	arg1	results					1061:1067	the computational results	1043:1067	the computational results	1043:1067	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	2	20	theme	drug-binding	603:614	arg1	interactions					616:627	drug-binding interactions	603:627	drug-binding interactions of CBG and BA	603:641	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	0	21	theme	Microbial	0:8	arg1	β-					17:18	Microbial cyclic β-	0:18	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.	0:169	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	21	theme	Microbial	0:8	arg1	1→6					26:28	1→6	26:28	1→6	26:28	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	21	theme	Microbial	0:8	arg1	1→3					20:22	1→3	20:22	1→3	20:22	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	22	dep	-glucans	30:37	arg1	studies					79:85	Interaction studies	67:85	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.	0:169	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	2	23	from	solubility	514:523	arg1	water					528:532	water	528:532	water	528:532	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	0	24	attach	isolated	112:119	arg1	japonicum					141:149	Bradyrhizobium japonicum	126:149	Bradyrhizobium japonicum	126:149	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	24	attach	isolated	112:119	arg1	acid					165:168	betulinic acid	155:168	betulinic acid	155:168	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	24	attach	isolated	112:119	arg2	β-glucans					102:110	cyclic β-glucans	95:110	cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid	95:168	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	0	25	theme	β-	17:18	arg1	-glucans					30:37	Microbial cyclic β-(1→3),(1→6)-glucans	0:37	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.	0:169	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	1	26	theme	promising	230:238	arg1	acid					181:184	Betulinic acid	171:184	Betulinic acid (BA)	171:189	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	26	theme	promising	230:238	arg1	drug					252:255	a very promising therapeutic drug	223:255	a very promising therapeutic drug with varied medicinal properties	223:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	0	27	theme	Interaction	67:77	arg1	studies					79:85	Interaction studies	67:85	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.	0:169	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	5	28	theme	powder	943:948	arg1	XRD					950:952	powder XRD	943:952	powder XRD	943:952	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	1	29	theme	Betulinic	171:179	arg1	triterpenoid					206:217	triterpenoid	206:217	triterpenoid	206:217	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	29	theme	Betulinic	171:179	arg1	BA					187:188	BA	187:188	BA	187:188	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	29	theme	Betulinic	171:179	arg1	acid					181:184	Betulinic acid	171:184	Betulinic acid (BA)	171:189	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	29	theme	Betulinic	171:179	arg1	drug					252:255	a very promising therapeutic drug	223:255	a very promising therapeutic drug with varied medicinal properties	223:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	30	theme	therapeutic	240:250	arg1	acid					181:184	Betulinic acid	171:184	Betulinic acid (BA)	171:189	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	30	theme	therapeutic	240:250	arg1	drug					252:255	a very promising therapeutic drug	223:255	a very promising therapeutic drug with varied medicinal properties	223:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	0	31	theme	cyclic	95:100	arg1	β-glucans					102:110	cyclic β-glucans	95:110	cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid	95:168	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	1	32	theme	low	342:344	arg1	bioavailability					346:360	consequentially low bioavailability	326:360	consequentially low bioavailability	326:360	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	2	33	theme	ATCC	468:471	arg1	10324					473:477	Bradyrhizobium japonicum ATCC 10324	443:477	Bradyrhizobium japonicum ATCC 10324 having a cavity structure	443:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	34	from	cyclooligosaccharides	409:429	arg1	water					528:532	water	528:532	water	528:532	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	35	theme	BA	640:641	arg1	interactions					616:627	drug-binding interactions	603:627	drug-binding interactions of CBG and BA	603:641	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	36	theme	Cyclic	363:368	arg1	β-					370:371	Cyclic β-	363:371	Cyclic β-(1→3)	363:376	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	36	theme	Cyclic	363:368	arg1	1→3					373:375	1→3	373:375	1→3	373:375	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	37	theme	japonicum	458:466	arg1	10324					473:477	Bradyrhizobium japonicum ATCC 10324	443:477	Bradyrhizobium japonicum ATCC 10324 having a cavity structure	443:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	38	theme	good	509:512	arg1	solubility					514:523	good solubility	509:523	good solubility in water	509:532	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	4	39	theme	1:3	859:861	arg1	CBG					863:865	1:2 and 1:3 CBG	851:865	1:2 and 1:3 CBG:BA	851:868	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	4	39	theme	1:3	859:861	arg1	compositions					830:841	three compositions	824:841	three compositions of 1:1	824:848	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	5	40	theme	UV-VIS	905:910	arg1	spectroscopy					912:923	UV-VIS spectroscopy	905:923	UV-VIS spectroscopy	905:923	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	4	41	dep	CBG	863:865	arg1	BA					867:868	BA	867:868	1:2 and 1:3 CBG:BA	851:868	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	1	42	contain	has	297:299	arg1	it					294:295	it	294:295	it	294:295	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	42	contain	has	297:299	arg2	solubility					311:320	low water solubility	301:320	low water solubility	301:320	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	42	contain	has	297:299	arg2	bioavailability					346:360	consequentially low bioavailability	326:360	consequentially low bioavailability	326:360	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	1	43	theme	varied	262:267	arg1	properties					279:288	varied medicinal properties	262:288	varied medicinal properties	262:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	4	44	theme	acid	792:795	arg1	complexes					797:805	the cyclic glucan-betulinic acid complexes	764:805	the cyclic glucan-betulinic acid complexes	764:805	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	5	45	theme	scanning	968:975	arg1	DSC					990:992	DSC	990:992	DSC	990:992	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	5	45	theme	scanning	968:975	arg1	calorimetry					977:987	differential scanning calorimetry	955:987	differential scanning calorimetry (DSC)	955:993	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	2	46	theme	CBG	632:634	arg1	interactions					616:627	drug-binding interactions	603:627	drug-binding interactions of CBG and BA	603:641	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	1	47	theme	medicinal	269:277	arg1	properties					279:288	varied medicinal properties	262:288	varied medicinal properties	262:288	Betulinic acid (BA), a pentacyclic triterpenoid, is a very promising therapeutic drug with varied medicinal properties but it has low water solubility and consequentially low bioavailability.
29898432	0	48	theme	Bradyrhizobium	126:139	arg1	japonicum					141:149	Bradyrhizobium japonicum	126:149	Bradyrhizobium japonicum	126:149	Microbial cyclic β-(1→3),(1→6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid.
29898432	5	49	theme	IR	926:927	arg1	spectroscopy					929:940	IR spectroscopy	926:940	IR spectroscopy	926:940	The complexes were analysed using UV-VIS spectroscopy, IR spectroscopy, powder XRD, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to confirm the computational results and consequently the encapsulation efficiency was found to be 9.53%.
29898432	2	50	theme	cavity	488:493	arg1	structure					495:503	a cavity structure	486:503	a cavity structure	486:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	51	from	β-	370:371	arg1	water					528:532	water	528:532	water	528:532	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	4	52	theme	1:1	846:848	arg1	CBG					863:865	1:2 and 1:3 CBG	851:865	1:2 and 1:3 CBG:BA	851:868	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	4	52	theme	1:1	846:848	arg1	compositions					830:841	three compositions	824:841	three compositions of 1:1	824:848	Then, the cyclic glucan-betulinic acid complexes were prepared in three compositions of 1:1, 1:2 and 1:3 CBG:BA.
29898432	3	53	dep	CBG	746:748	arg1	the					742:744	the	742:744	the	742:744	First, in silico approach was employed to study the scope of any interaction between the CBG and BA.
29898432	2	54	theme	microbial	399:407	arg1	cyclooligosaccharides					409:429	microbial cyclooligosaccharides	399:429	microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure	399:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	55	theme	1→6	379:381	arg1	-glucans					383:390	(1→6)-glucans	378:390	(1→6)-glucans (CBG)	378:396	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	55	theme	1→6	379:381	arg1	CBG					393:395	CBG	393:395	CBG	393:395	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29898432	2	56	theme	Bradyrhizobium	443:456	arg1	japonicum					458:466	Bradyrhizobium japonicum	443:466	Bradyrhizobium japonicum ATCC 10324 having a cavity structure	443:503	Cyclic β-(1→3),(1→6)-glucans (CBG), microbial cyclooligosaccharides produced by Bradyrhizobium japonicum ATCC 10324 having a cavity structure and good solubility in water have been tested for their ability to encapsulate betulinic acid and drug-binding interactions of CBG and BA were studied.
29368511	4	0	theme	necessary	1025:1033	arg1	isolation					989:997	cell wall isolation	979:997	cell wall isolation	979:997	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	3	1	theme	bacterial	661:669	arg1	cultures					671:678	bacterial cultures	661:678	bacterial cultures with incomplete amino acid labeling	661:714	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	2	2	theme	external	338:345	arg1	stimuli					347:353	external stimuli	338:353	external stimuli	338:353	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	5	3	theme	Lys	1215:1217	arg1	%					1252:1252	98.33 ± 0.05%	1240:1252	98.33 ± 0.05%	1240:1252	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	3	theme	Lys	1215:1217	arg1	enrichment					1228:1237	incomplete heavy Lys isotopic enrichment	1198:1237	incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%)	1198:1253	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	4	4	theme	planktonic	855:864	arg1	bacteria					866:873	planktonic bacteria	855:873	planktonic bacteria	855:873	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	5	theme	liquid	1039:1044	arg1	chromatography					1046:1059	liquid chromatography	1039:1059	liquid chromatography	1039:1059	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	3	6	theme	amino	696:700	arg1	labeling					707:714	incomplete amino acid labeling	685:714	incomplete amino acid labeling	685:714	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	0	7	theme	Peptidoglycan	0:12	arg1	Analysis					28:35	Peptidoglycan Compositional Analysis	0:35	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.	0:134	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	1	8	theme	major	160:164	arg1	component					166:174	a major component	158:174	a major component of the cell wall in Enterococcus faecalis	158:216	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	1	8	theme	major	160:164	arg1	Peptidoglycan					136:148	Peptidoglycan	136:148	Peptidoglycan (PG)	136:153	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	6	9	theme	PG	1311:1312	arg1	ions					1323:1326	PG fragment ions	1311:1326	PG fragment ions from isolated cell walls of planktonic and biofilm samples	1311:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	1	10	theme	cell	183:186	arg1	wall					188:191	the cell wall	179:191	the cell wall	179:191	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	7	11	from	PG	1439:1440	arg1	biofilm					1445:1451	biofilm	1445:1451	biofilm	1445:1451	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	12	theme	MurNAc	1587:1592	arg1	O-acetylation					1570:1582	O-acetylation	1570:1582	O-acetylation of MurNAc	1570:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	13	theme	O-acetylation	1570:1582	arg1	level					1473:1477	an increased level	1460:1477	an increased level of PG cross-linking	1460:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	13	theme	O-acetylation	1570:1582	arg1	number					1612:1617	an increased number	1599:1617	an increased number of stem modifications	1599:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	13	theme	O-acetylation	1570:1582	arg1	level					1561:1565	a decreased level	1549:1565	a decreased level of O-acetylation of MurNAc	1549:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	13	theme	O-acetylation	1570:1582	arg1	level					1513:1517	an increased level	1500:1517	an increased level of N-deacetylation of GlcNAc	1500:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	3	14	theme	isotope	584:590	arg1	labeling					592:599	"stable isotope labeling	576:599	"stable isotope labeling by amino acids in bacterial culture" (SILAB)	576:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	4	15	theme	abundance	888:896	arg1	l-Lys					898:902	natural abundance l-Lys	880:902	natural abundance l-Lys	880:902	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	3	16	theme	bacterial	619:627	arg1	culture					629:635	bacterial culture"	619:636	bacterial culture" (SILAB)	619:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	3	16	theme	bacterial	619:627	arg1	SILAB					639:643	SILAB	639:643	SILAB	639:643	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	1	17	from	component	166:174	arg1	faecalis					209:216	Enterococcus faecalis	196:216	Enterococcus faecalis	196:216	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	4	18	with	biofilm	805:811	arg1	l-[13C6,2D9,15N2					829:844	l-[13C6,2D9,15N2	829:844	l-[13C6,2D9,15N2	829:844	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	18	with	biofilm	805:811	arg1	Lys					824:826	heavy Lys	818:826	heavy Lys (l-[13C6,2D9,15N2]Lys)	818:849	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	6	19	theme	planktonic	1356:1365	arg1	samples					1379:1385	planktonic and biofilm samples	1356:1385	planktonic and biofilm samples	1356:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	7	20	theme	GlcNAc	1541:1546	arg1	N-deacetylation					1522:1536	N-deacetylation	1522:1536	N-deacetylation of GlcNAc	1522:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	21	theme	N-deacetylation	1522:1536	arg1	level					1473:1477	an increased level	1460:1477	an increased level of PG cross-linking	1460:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	21	theme	N-deacetylation	1522:1536	arg1	number					1612:1617	an increased number	1599:1617	an increased number of stem modifications	1599:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	21	theme	N-deacetylation	1522:1536	arg1	level					1561:1565	a decreased level	1549:1565	a decreased level of O-acetylation of MurNAc	1549:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	21	theme	N-deacetylation	1522:1536	arg1	level					1513:1517	an increased level	1500:1517	an increased level of N-deacetylation of GlcNAc	1500:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	2	22	theme	PG	240:241	arg1	composition					243:253	PG composition	240:253	PG composition	240:253	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	0	23	theme	Amino	100:104	arg1	Acids					106:110	Amino Acids	100:110	Amino Acids in a Bacterial Culture	100:133	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	4	24	theme	bacteria	934:941	arg1	bacteria					934:941	bacteria	934:941	bacteria	934:941	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	24	theme	bacteria	934:941	arg1	amounts					923:929	equal amounts	917:929	equal amounts of bacteria	917:941	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	3	25	from	acids	610:614	arg1	culture					629:635	bacterial culture"	619:636	bacterial culture" (SILAB)	619:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	3	25	from	acids	610:614	arg1	SILAB					639:643	SILAB	639:643	SILAB	639:643	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	6	26	theme	ions	1323:1326	arg1	pairs					1302:1306	Forty-seven pairs	1290:1306	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples	1290:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	7	27	theme	stem	1622:1625	arg1	modifications					1627:1639	stem modifications	1622:1639	stem modifications	1622:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	4	28	theme	comparative	722:732	arg1	analysis					734:741	This comparative analysis	717:741	This comparative analysis by mass spectrometry	717:762	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	29	dep	l-[13C6,2D9,15N2	829:844	arg1	Lys					846:848	Lys	846:848	Lys	846:848	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	2	30	theme	chemical	413:420	arg1	modifications					422:434	various chemical modifications	405:434	various chemical modifications to PG-repeat subunits	405:456	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	0	31	theme	Enterococcus	40:51	arg1	Biofilm					62:68	Enterococcus faecalis Biofilm	40:68	Enterococcus faecalis Biofilm	40:68	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	7	32	theme	PG	1482:1483	arg1	cross-linking					1485:1497	PG cross-linking	1482:1497	PG cross-linking	1482:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	4	33	theme	wall	984:987	arg1	isolation					989:997	cell wall isolation	979:997	cell wall isolation	979:997	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	0	34	theme	Stable	73:78	arg1	Labeling					88:95	Stable Isotope Labeling	73:95	Stable Isotope Labeling by Amino Acids in a Bacterial Culture	73:133	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	4	35	theme	heavy	818:822	arg1	l-[13C6,2D9,15N2					829:844	l-[13C6,2D9,15N2	829:844	l-[13C6,2D9,15N2	829:844	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	35	theme	heavy	818:822	arg1	Lys					824:826	heavy Lys	818:826	heavy Lys (l-[13C6,2D9,15N2]Lys)	818:849	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	3	36	theme	PG	491:492	arg1	composition					494:504	the PG composition	487:504	the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB)	487:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	0	37	theme	Biofilm	62:68	arg1	Analysis					28:35	Peptidoglycan Compositional Analysis	0:35	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.	0:134	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	5	38	theme	correction	1161:1170	arg1	factors					1172:1178	correction factors	1161:1178	correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%)	1161:1287	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	4	39	theme	mass	1065:1068	arg1	spectrometry					1070:1081	mass spectrometry	1065:1081	mass spectrometry	1065:1081	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	1	40	theme	Enterococcus	196:207	arg1	faecalis					209:216	Enterococcus faecalis	196:216	Enterococcus faecalis	196:216	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	3	41	theme	planktonic	530:539	arg1	bacteria					541:548	planktonic bacteria	530:548	planktonic bacteria	530:548	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	3	42	theme	faecalis	512:519	arg1	composition					494:504	the PG composition	487:504	the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB)	487:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	5	43	theme	±	1246:1246	arg1	%					1252:1252	98.33 ± 0.05%	1240:1252	98.33 ± 0.05%	1240:1252	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	43	theme	±	1246:1246	arg1	enrichment					1228:1237	incomplete heavy Lys isotopic enrichment	1198:1237	incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%)	1198:1253	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	3	44	theme	incomplete	685:694	arg1	labeling					707:714	incomplete amino acid labeling	685:714	incomplete amino acid labeling	685:714	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	3	45	with	cultures	671:678	arg1	labeling					707:714	incomplete amino acid labeling	685:714	incomplete amino acid labeling	685:714	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	5	46	theme	heavy	1209:1213	arg1	%					1252:1252	98.33 ± 0.05%	1240:1252	98.33 ± 0.05%	1240:1252	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	46	theme	heavy	1209:1213	arg1	enrichment					1228:1237	incomplete heavy Lys isotopic enrichment	1198:1237	incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%)	1198:1253	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	47	theme	isotopic	1219:1226	arg1	%					1252:1252	98.33 ± 0.05%	1240:1252	98.33 ± 0.05%	1240:1252	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	47	theme	isotopic	1219:1226	arg1	enrichment					1228:1237	incomplete heavy Lys isotopic enrichment	1198:1237	incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%)	1198:1253	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	2	48	theme	crucial	264:270	arg1	insights					272:279	crucial insights	264:279	crucial insights into the bacterium's cellular functions	264:319	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	6	49	theme	biofilm	1371:1377	arg1	samples					1379:1385	planktonic and biofilm samples	1356:1385	planktonic and biofilm samples	1356:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	3	50	theme	acid	702:705	arg1	labeling					707:714	incomplete amino acid labeling	685:714	incomplete amino acid labeling	685:714	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	2	51	theme	cellular	302:309	arg1	functions					311:319	the bacterium's cellular functions	286:319	the bacterium's cellular functions	286:319	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	6	52	theme	fragment	1314:1321	arg1	ions					1323:1326	PG fragment ions	1311:1326	PG fragment ions from isolated cell walls of planktonic and biofilm samples	1311:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	2	53	theme	Accurate	219:226	arg1	analysis					228:235	Accurate analysis	219:235	Accurate analysis of PG composition	219:253	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	1	54	theme	wall	188:191	arg1	component					166:174	a major component	158:174	a major component of the cell wall in Enterococcus faecalis	158:216	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	1	54	theme	wall	188:191	arg1	Peptidoglycan					136:148	Peptidoglycan	136:148	Peptidoglycan (PG)	136:153	Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis.
29368511	6	55	theme	Forty-seven	1290:1300	arg1	pairs					1302:1306	Forty-seven pairs	1290:1306	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples	1290:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	3	56	theme	stable	577:582	arg1	labeling					592:599	"stable isotope labeling	576:599	"stable isotope labeling by amino acids in bacterial culture" (SILAB)	576:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	7	57	theme	decreased	1551:1559	arg1	level					1561:1565	a decreased level	1549:1565	a decreased level of O-acetylation of MurNAc	1549:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	2	58	theme	composition	243:253	arg1	analysis					228:235	Accurate analysis	219:235	Accurate analysis of PG composition	219:253	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	4	59	theme	natural	880:886	arg1	l-Lys					898:902	natural abundance l-Lys	880:902	natural abundance l-Lys	880:902	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	0	60	from	Acids	106:110	arg1	Culture					127:133	a Bacterial Culture	115:133	a Bacterial Culture	115:133	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	5	61	theme	±	1280:1280	arg1	%					1286:1286	83.23 ± 1.16%	1274:1286	83.23 ± 1.16%	1274:1286	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	61	theme	±	1280:1280	arg1	incorporation					1259:1271	incorporation	1259:1271	incorporation (83.23 ± 1.16%)	1259:1287	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	0	62	theme	Isotope	80:86	arg1	Labeling					88:95	Stable Isotope Labeling	73:95	Stable Isotope Labeling by Amino Acids in a Bacterial Culture	73:133	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	3	63	theme	amino	604:608	arg1	acids					610:614	amino acids	604:614	amino acids in bacterial culture" (SILAB)	604:644	We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing "stable isotope labeling by amino acids in bacterial culture" (SILAB), optimized for bacterial cultures with incomplete amino acid labeling.
29368511	6	64	theme	isolated	1333:1340	arg1	walls					1347:1351	isolated cell walls	1333:1351	isolated cell walls of planktonic and biofilm samples	1333:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	6	65	from	walls	1347:1351	arg1	ions					1323:1326	PG fragment ions	1311:1326	PG fragment ions from isolated cell walls of planktonic and biofilm samples	1311:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	6	65	from	walls	1347:1351	arg1	pairs					1302:1306	Forty-seven pairs	1290:1306	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples	1290:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	0	66	theme	Bacterial	117:125	arg1	Culture					127:133	a Bacterial Culture	115:133	a Bacterial Culture	115:133	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	6	67	theme	cell	1342:1345	arg1	walls					1347:1351	isolated cell walls	1333:1351	isolated cell walls of planktonic and biofilm samples	1333:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	5	68	theme	muropeptide	1132:1142	arg1	abundances					1144:1153	muropeptide abundances	1132:1153	muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%)	1132:1287	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	7	69	theme	increased	1503:1511	arg1	level					1513:1517	an increased level	1500:1517	an increased level of N-deacetylation of GlcNAc	1500:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	4	70	theme	equal	917:921	arg1	bacteria					934:941	bacteria	934:941	bacteria	934:941	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	70	theme	equal	917:921	arg1	amounts					923:929	equal amounts	917:929	equal amounts of bacteria	917:941	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	4	71	theme	mass	746:749	arg1	spectrometry					751:762	mass spectrometry	746:762	mass spectrometry	746:762	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	5	72	theme	analytical	1087:1096	arg1	method					1098:1103	An analytical method	1084:1103	An analytical method	1084:1103	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	0	73	theme	Compositional	14:26	arg1	Analysis					28:35	Peptidoglycan Compositional Analysis	0:35	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.	0:134	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	7	74	theme	modifications	1627:1639	arg1	level					1473:1477	an increased level	1460:1477	an increased level of PG cross-linking	1460:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	74	theme	modifications	1627:1639	arg1	number					1612:1617	an increased number	1599:1617	an increased number of stem modifications	1599:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	74	theme	modifications	1627:1639	arg1	level					1561:1565	a decreased level	1549:1565	a decreased level of O-acetylation of MurNAc	1549:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	74	theme	modifications	1627:1639	arg1	level					1513:1517	an increased level	1500:1517	an increased level of N-deacetylation of GlcNAc	1500:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	2	75	theme	PG-repeat	439:447	arg1	subunits					449:456	PG-repeat subunits	439:456	PG-repeat subunits	439:456	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	6	76	theme	SILAB	1405:1409	arg1	analysis					1411:1418	SILAB analysis	1405:1418	SILAB analysis	1405:1418	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	6	77	from	pairs	1302:1306	arg1	walls					1347:1351	isolated cell walls	1333:1351	isolated cell walls of planktonic and biofilm samples	1333:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	4	78	theme	mutanolysin	1003:1013	arg1	digestion					1015:1023	mutanolysin digestion	1003:1023	mutanolysin digestion	1003:1023	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	2	79	theme	various	405:411	arg1	modifications					422:434	various chemical modifications	405:434	various chemical modifications to PG-repeat subunits	405:456	Accurate analysis of PG composition provides crucial insights into the bacterium's cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits.
29368511	0	80	theme	faecalis	53:60	arg1	Biofilm					62:68	Enterococcus faecalis Biofilm	40:68	Enterococcus faecalis Biofilm	40:68	Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.
29368511	5	81	theme	incomplete	1198:1207	arg1	%					1252:1252	98.33 ± 0.05%	1240:1252	98.33 ± 0.05%	1240:1252	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	5	81	theme	incomplete	1198:1207	arg1	enrichment					1228:1237	incomplete heavy Lys isotopic enrichment	1198:1237	incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%)	1198:1253	An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%).
29368511	7	82	theme	increased	1602:1610	arg1	number					1612:1617	an increased number	1599:1617	an increased number of stem modifications	1599:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	6	83	theme	samples	1379:1385	arg1	walls					1347:1351	isolated cell walls	1333:1351	isolated cell walls of planktonic and biofilm samples	1333:1385	Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis.
29368511	7	84	theme	cross-linking	1485:1497	arg1	level					1473:1477	an increased level	1460:1477	an increased level of PG cross-linking	1460:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	84	theme	cross-linking	1485:1497	arg1	number					1612:1617	an increased number	1599:1617	an increased number of stem modifications	1599:1639	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	84	theme	cross-linking	1485:1497	arg1	level					1561:1565	a decreased level	1549:1565	a decreased level of O-acetylation of MurNAc	1549:1592	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	7	84	theme	cross-linking	1485:1497	arg1	level					1513:1517	an increased level	1500:1517	an increased level of N-deacetylation of GlcNAc	1500:1546	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29368511	4	85	theme	cell	979:982	arg1	isolation					989:997	cell wall isolation	979:997	cell wall isolation	979:997	This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry.
29368511	7	86	theme	increased	1463:1471	arg1	level					1473:1477	an increased level	1460:1477	an increased level of PG cross-linking	1460:1497	We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases.
29377599	1	0	theme	Dynamic	315:321	arg1	Atomization					323:333	Electro Dynamic Atomization	307:333	Electro Dynamic Atomization (EDA)	307:339	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	1	0	theme	Dynamic	315:321	arg1	EDA					336:338	EDA	336:338	EDA	336:338	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	2	1	theme	massive	555:561	arg1	release					563:569	a massive release	553:569	a massive release along the intestine	553:589	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	1	2	theme	structure-inner	156:170	arg1	core					172:175	core/shell structure-inner core	145:175	core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	145:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	1	3	theme	chitosan	236:243	arg1	layer					227:231	a micrometric layer	213:231	a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	213:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	3	4	theme	different	662:670	arg1	conditions					675:684	different pH conditions	662:684	different pH conditions	662:684	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	3	5	theme	water	640:644	arg1	retention					646:654	water retention	640:654	water retention	640:654	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	5	6	theme	delayed	1111:1117	arg1	administration					1124:1137	a delayed oral administration	1109:1137	a delayed oral administration of anti-inflammatory drugs	1109:1164	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	5	7	theme	strong	1065:1070	arg1	potential					1072:1080	strong potential	1065:1080	strong potential	1065:1080	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	7	8	theme	Part	1217:1220	arg1	B					1222:1222	Part B	1217:1222	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.	1198:1261	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	4	9	theme	physiological	835:847	arg1	enzymes					849:855	physiological enzymes	835:855	physiological enzymes	835:855	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	4	10	with	fluids	812:817	arg1	enzymes					849:855	physiological enzymes	835:855	physiological enzymes	835:855	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	5	11	theme	obtained	1004:1011	arg1	results					1013:1019	The obtained results	1000:1019	The obtained results	1000:1019	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	2	12	theme	Ketoprofen	487:496	arg1	drugs-that					472:481	the drugs-that	468:481	the drugs-that is	468:484	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	2	12	theme	Ketoprofen	487:496	arg1	KL					508:509	KL	508:509	KL	508:509	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	2	12	theme	Ketoprofen	487:496	arg1	Lysinate					498:505	Ketoprofen Lysinate	487:505	Ketoprofen Lysinate (KL)-along the gastric tract	487:534	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	4	13	dep	%	917:917	arg1	ca.					911:913	ca.	911:913	ca.	911:913	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	2	14	contain	possess	419:425	arg1	microspheres					406:417	CA based microspheres	397:417	CA based microspheres	397:417	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	2	14	contain	possess	419:425	arg2	capability					436:445	a unique capability	427:445	a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract	427:534	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	4	15	from	pH	993:994	arg1	%					988:988	80%	986:988	80%	986:988	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	4	16	theme	first	900:904	arg1	h					908:908	the first 2 h	896:908	the first 2 h (ca. 20% at pH 2)	896:926	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	3	17	theme	pH	672:673	arg1	conditions					675:684	different pH conditions	662:684	different pH conditions	662:684	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	1	18	with	carrier	132:138	arg1	core					172:175	core/shell structure-inner core	145:175	core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	145:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	1	19	theme	cellulose	180:188	arg1	acetate					190:196	cellulose acetate	180:196	cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	180:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	1	19	theme	cellulose	180:188	arg1	CA					199:200	CA	199:200	CA	199:200	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	6	20	dep	©	1167:1167	arg1	Inc.					1193:1196	Inc.	1193:1196	Inc.	1193:1196	© 2018 Wiley Periodicals, Inc.
29377599	1	21	theme	layer-by-layer	345:358	arg1	technique					366:374	layer-by-layer (LbL) technique	345:374	layer-by-layer (LbL) technique	345:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	5	22	theme	CA-based	1038:1045	arg1	microspheres					1047:1058	CA-based microspheres	1038:1058	CA-based microspheres	1038:1058	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	1	23	theme	acetate	190:196	arg1	core					172:175	core/shell structure-inner core	145:175	core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	145:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	24	dep	In	760:761	arg1	vitro					763:767	vitro	763:767	vitro	763:767	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	2	25	theme	based	400:404	arg1	microspheres					406:417	CA based microspheres	397:417	CA based microspheres	397:417	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	2	26	theme	CA	397:398	arg1	microspheres					406:417	CA based microspheres	397:417	CA based microspheres	397:417	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	3	27	theme	drug	697:700	arg1	encapsulation					702:714	drug encapsulation	697:714	drug encapsulation	697:714	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	4	28	theme	moderate	865:872	arg1	release					874:880	a moderate release	863:880	a moderate release of LSK	863:887	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	2	29	theme	unique	429:434	arg1	capability					436:445	a unique capability	427:445	a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract	427:534	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	1	30	theme	integrated	272:281	arg1	process					283:289	an integrated process	269:289	an integrated process	269:289	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	31	theme	LSK	885:887	arg1	release					874:880	a moderate release	863:880	a moderate release of LSK	863:887	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	1	32	theme	LbL	361:363	arg1	technique					366:374	layer-by-layer (LbL) technique	345:374	layer-by-layer (LbL) technique	345:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	33	theme	gastric	790:796	arg1	fluids					812:817	simulated gastric and intestine fluids	780:817	simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes	780:855	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	7	34	dep	Biomater	1230:1237	arg1	2636-2644					1246:1254	2636-2644	1246:1254	2636-2644	1246:1254	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	7	35	dep	Res	1213:1215	arg1	2018					1257:1260	2018	1257:1260	2018	1257:1260	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	7	35	dep	Res	1213:1215	arg1	106B					1240:1243	106B	1240:1243	106B	1240:1243	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	7	35	dep	Res	1213:1215	arg1	B					1222:1222	Part B	1217:1222	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.	1198:1261	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	7	35	dep	Res	1213:1215	arg1	Biomater					1230:1237	Biomater	1230:1237	Biomater	1230:1237	J Biomed Mater Res Part B: Appl Biomater, 106B: 2636-2644, 2018.
29377599	0	36	theme	oral	44:47	arg1	administration					49:62	oral administration	44:62	oral administration of Ketoprofen Lysinate	44:85	Core/shell cellulose-based microspheres for oral administration of Ketoprofen Lysinate.
29377599	4	37	dep	%	988:988	arg1	ca.					982:984	ca.	982:984	ca.	982:984	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	0	38	theme	Ketoprofen	67:76	arg1	Lysinate					78:85	Ketoprofen Lysinate	67:85	Ketoprofen Lysinate	67:85	Core/shell cellulose-based microspheres for oral administration of Ketoprofen Lysinate.
29377599	4	39	from	pH	922:923	arg1	%					917:917	20%	915:917	20%	915:917	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	3	40	theme	CS	592:593	arg1	shell					595:599	CS shell	592:599	CS shell	592:599	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	4	41	theme	intestine	802:810	arg1	fluids					812:817	simulated gastric and intestine fluids	780:817	simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes	780:855	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	2	42	dep	drugs-that	472:481	arg1	is					483:484	is	483:484	is	483:484	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	3	43	theme	drug	737:740	arg1	kinetics					750:757	drug release kinetics	737:757	drug release kinetics	737:757	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	4	44	dep	followed	929:936	arg1	%					988:988	80%	986:988	80%	986:988	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	4	45	theme	next	972:975	arg1	h					979:979	the next 6 h	968:979	the next 6 h	968:979	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	3	46	theme	release	742:748	arg1	kinetics					750:757	drug release kinetics	737:757	drug release kinetics	737:757	CS shell slightly influences the morphology and water retention under different pH conditions, improving drug encapsulation without compromising drug release kinetics.
29377599	2	47	theme	gastric	522:528	arg1	tract					530:534	the gastric tract	518:534	the gastric tract	518:534	We demonstrate that CA based microspheres possess a unique capability to relevantly retain the drugs-that is, Ketoprofen Lysinate (KL)-along the gastric tract, while providing a massive release along the intestine.
29377599	1	48	theme	new	128:130	arg1	carrier					132:138	a new carrier	126:138	a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	126:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	49	theme	In	760:761	arg1	studies					769:775	In vitro studies	760:775	In vitro studies	760:775	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	1	50	theme	carrier	132:138	arg1	fabrication					111:121	the fabrication	107:121	the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	107:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	5	51	theme	anti-inflammatory	1142:1158	arg1	drugs					1160:1164	anti-inflammatory drugs	1142:1164	anti-inflammatory drugs	1142:1164	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	1	52	theme	micrometric	215:225	arg1	layer					227:231	a micrometric layer	213:231	a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	213:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	5	53	theme	oral	1119:1122	arg1	administration					1124:1137	a delayed oral administration	1109:1137	a delayed oral administration of anti-inflammatory drugs	1109:1164	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	4	54	from	studies	769:775	arg1	fluids					812:817	simulated gastric and intestine fluids	780:817	simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes	780:855	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	5	55	theme	drugs	1160:1164	arg1	administration					1124:1137	a delayed oral administration	1109:1137	a delayed oral administration of anti-inflammatory drugs	1109:1164	The obtained results demonstrate that CA-based microspheres hold strong potential to be used as carriers for a delayed oral administration of anti-inflammatory drugs.
29377599	0	56	theme	Lysinate	78:85	arg1	administration					49:62	oral administration	44:62	oral administration of Ketoprofen Lysinate	44:85	Core/shell cellulose-based microspheres for oral administration of Ketoprofen Lysinate.
29377599	1	57	theme	Electro	307:313	arg1	Atomization					323:333	Electro Dynamic Atomization	307:333	Electro Dynamic Atomization (EDA)	307:339	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	1	57	theme	Electro	307:313	arg1	EDA					336:338	EDA	336:338	EDA	336:338	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	58	dep	fluids	812:817	arg1	SGF					820:822	SGF	820:822	SGF	820:822	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	4	58	dep	fluids	812:817	arg1	SIF					825:827	SIF	825:827	SIF	825:827	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
29377599	1	59	theme	core/shell	145:154	arg1	core					172:175	core/shell structure-inner core	145:175	core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique	145:374	Herein, we propose the fabrication of a new carrier with core/shell structure-inner core of cellulose acetate (CA) coated by a micrometric layer of chitosan (CS)-fabricated through an integrated process, which combines Electro Dynamic Atomization (EDA) and layer-by-layer (LbL) technique.
29377599	4	60	theme	sustained	943:951	arg1	release					953:959	a sustained release	941:959	a sustained release during the next 6 h	941:979	In vitro studies in simulated gastric and intestine fluids (SGF, SIF) with physiological enzymes, show a moderate release of LSK during the first 2 h (ca. 20% at pH 2), followed by a sustained release during the next 6 h (ca. 80% at pH 7).
31234073	2	0	theme	characterization	342:357	arg1	series					332:337	a series	330:337	a series of characterization	330:357	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	0	1	theme	bacterial	80:88	arg1	cellulose					90:98	bacterial cellulose	80:98	bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell	80:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	3	2	theme	P	493:493	arg1	doping					476:481	the doping	472:481	the doping of Cu and P	472:493	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	2	3	with	network	412:418	arg1	area					444:447	a specific surface area	425:447	a specific surface area of 580.09 m2/g	425:462	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	5	4	theme	alternative	915:925	arg1	catalysts					941:949	alternative commercial Pt catalysts	915:949	alternative commercial Pt catalysts	915:949	This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
31234073	4	5	theme	current	719:725	arg1	density					727:733	current density	719:733	current density of 1177.31 mW/m2 and 6.73 A/m2	719:764	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	1	6	theme	high-temperature	254:269	arg1	pyrolysis					271:279	high-temperature pyrolysis	254:279	high-temperature pyrolysis	254:279	In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts.
31234073	1	7	used	used	285:288	arg2	cellulose					189:197	bacterial cellulose	179:197	bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis	179:279	In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts.
31234073	3	8	theme	active	501:506	arg1	sites					508:512	more active sites	496:512	more active sites	496:512	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	2	9	theme	580.09 m2/g	452:462	arg1	area					444:447	a specific surface area	425:447	a specific surface area of 580.09 m2/g	425:462	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	5	10	theme	Pt	938:939	arg1	catalysts					941:949	alternative commercial Pt catalysts	915:949	alternative commercial Pt catalysts	915:949	This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
31234073	0	11	theme	excellent	3:11	arg1	modifier					35:42	An excellent alternative composite modifier	0:42	An excellent alternative composite modifier for cathode catalysts	0:64	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	4	12	theme	output	702:707	arg1	power					709:713	the maximum output power	690:713	the maximum output power	690:713	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	4	13	theme	air	649:651	arg1	surface					661:667	the air cathode surface	645:667	the air cathode surface of the MFC	645:678	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	4	14	theme	maximum	694:700	arg1	power					709:713	the maximum output power	690:713	the maximum output power	690:713	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	2	15	theme	synthesized	364:374	arg1	catalyst					376:383	the synthesized catalyst	360:383	the synthesized catalyst	360:383	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	2	16	theme	surface	436:442	arg1	area					444:447	a specific surface area	425:447	a specific surface area of 580.09 m2/g	425:462	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	5	17	theme	bioelectrical	874:886	arg1	generation					888:897	bioelectrical generation	874:897	bioelectrical generation in MFC	874:904	This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
31234073	4	18	theme	prepared	613:620	arg1	catalyst					622:629	The prepared catalyst	609:629	The prepared catalyst	609:629	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	2	19	theme	specific	427:434	arg1	area					444:447	a specific surface area	425:447	a specific surface area of 580.09 m2/g	425:462	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	4	20	theme	1177.31 mW/m2	738:750	arg1	power					709:713	the maximum output power	690:713	the maximum output power	690:713	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	4	20	theme	1177.31 mW/m2	738:750	arg1	density					727:733	current density	719:733	current density of 1177.31 mW/m2 and 6.73 A/m2	719:764	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	1	21	theme	bacterial	179:187	arg1	cellulose					189:197	bacterial cellulose	179:197	bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis	179:279	In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts.
31234073	4	22	theme	MFC	676:678	arg1	surface					661:667	the air cathode surface	645:667	the air cathode surface of the MFC	645:678	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	0	23	theme	composite	25:33	arg1	modifier					35:42	An excellent alternative composite modifier	0:42	An excellent alternative composite modifier for cathode catalysts	0:64	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	2	24	theme	three-dimensional	394:410	arg1	network					412:418	a three-dimensional network	392:418	a three-dimensional network with a specific surface area of 580.09 m2/g	392:462	After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m2/g.
31234073	1	25	theme	MFC	301:303	arg1	catalysts					313:321	MFC cathode catalysts	301:321	MFC cathode catalysts	301:321	In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts.
31234073	0	26	theme	microbial	143:151	arg1	cell					158:161	microbial fuel cell	143:161	microbial fuel cell	143:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	3	27	theme	bacterial	543:551	arg1	cellulose					553:561	bacterial cellulose	543:561	bacterial cellulose	543:561	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	0	28	theme	cathode	48:54	arg1	catalysts					56:64	cathode catalysts	48:64	cathode catalysts	48:64	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	1	29	theme	cathode	305:311	arg1	catalysts					313:321	MFC cathode catalysts	301:321	MFC cathode catalysts	301:321	In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts.
31234073	3	30	theme	cellulose	553:561	arg1	pores					534:538	the pores	530:538	the pores of bacterial cellulose	530:561	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	3	31	theme	Cu	486:487	arg1	doping					476:481	the doping	472:481	the doping of Cu and P	472:493	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	3	32	theme	catalytic	589:597	arg1	activity					599:606	catalytic activity	589:606	catalytic activity	589:606	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	0	33	from	Cu	111:112	arg1	cell					158:161	microbial fuel cell	143:161	microbial fuel cell	143:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	4	34	theme	6.73 A/m2	756:764	arg1	power					709:713	the maximum output power	690:713	the maximum output power	690:713	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	4	34	theme	6.73 A/m2	756:764	arg1	density					727:733	current density	719:733	current density of 1177.31 mW/m2 and 6.73 A/m2	719:764	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	0	35	from	utilization	128:138	arg1	cell					158:161	microbial fuel cell	143:161	microbial fuel cell	143:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	0	36	theme	fuel	153:156	arg1	cell					158:161	microbial fuel cell	143:161	microbial fuel cell	143:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	4	37	theme	cathode	653:659	arg1	surface					661:667	the air cathode surface	645:667	the air cathode surface of the MFC	645:678	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	5	38	from	generation	888:897	arg1	MFC					902:904	MFC	902:904	MFC	902:904	This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
31234073	5	39	theme	commercial	927:936	arg1	catalysts					941:949	alternative commercial Pt catalysts	915:949	alternative commercial Pt catalysts	915:949	This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
31234073	4	40	dep	Pt	813:814	arg1	1044.93 mW/m2					817:829	1044.93 mW/m2	817:829	1044.93 mW/m2	817:829	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	4	40	dep	Pt	813:814	arg1	6.02 A/m2					835:843	6.02 A/m2	835:843	6.02 A/m2	835:843	The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m2 and 6.73 A/m2, respectively, which were higher than those of Pt (1044.93 mW/m2 and 6.02 A/m2).
31234073	0	41	theme	alternative	13:23	arg1	modifier					35:42	An excellent alternative composite modifier	0:42	An excellent alternative composite modifier for cathode catalysts	0:64	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31234073	3	42	theme	more	496:499	arg1	sites					508:512	more active sites	496:512	more active sites	496:512	Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity.
31234073	0	43	from	P	118:118	arg1	cell					158:161	microbial fuel cell	143:161	microbial fuel cell	143:161	An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.
31771849	4	0	from	self-reduction	596:609	arg1	process					707:713	the calcination process	691:713	the calcination process	691:713	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	1	1	theme	different	279:287	arg1	temperatures					289:300	different temperatures	279:300	different temperatures under nitrogen atmosphere	279:326	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	6	2	theme	carbon	1228:1233	arg1	synergistic					1280:1290	synergistic	1280:1290	synergistic	1280:1290	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	2	theme	carbon	1228:1233	arg1	adsorption					1214:1223	the adsorption	1210:1223	the adsorption of carbon and reduction of Fe0/Fe3O4 toward U	1210:1269	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	2	theme	carbon	1228:1233	arg1	VI					1271:1272	VI	1271:1272	VI	1271:1272	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	7	3	theme	U	1426:1426	arg1	adsorption					1432:1441	U(VI) adsorption	1426:1441	U(VI) adsorption	1426:1441	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	1	4	theme	Novel	137:141	arg1	composites					155:164	Novel iron/carbon composites	137:164	Novel iron/carbon composites	137:164	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	8	5	theme	iron	1632:1635	arg1	resource					1637:1644	a promising iron resource	1620:1644	a promising iron resource for the preparation of iron/carbon composites	1620:1690	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	8	5	theme	iron	1632:1635	arg1	Fe2O3					1610:1614	the Fe2O3	1606:1614	the Fe2O3	1606:1614	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	8	6	theme	valuable	1557:1564	arg1	adsorbents					1566:1575	valuable adsorbents	1557:1575	valuable adsorbents in environmental cleanup	1557:1600	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	8	6	theme	valuable	1557:1564	arg1	composites					1525:1534	the iron/carbon composites	1509:1534	the iron/carbon composites	1509:1534	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	4	7	theme	strong	728:733	arg1	magnetism					735:743	the strong magnetism	724:743	the strong magnetism of the composites	724:761	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	8	8	theme	environmental	1580:1592	arg1	cleanup					1594:1600	environmental cleanup	1580:1600	environmental cleanup	1580:1600	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	1	9	theme	nitrogen	308:315	arg1	atmosphere					317:326	nitrogen atmosphere	308:326	nitrogen atmosphere	308:326	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	6	10	dep	synergistic	1280:1290	arg1	synergistic					1280:1290	synergistic	1280:1290	synergistic	1280:1290	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	10	dep	synergistic	1280:1290	arg1	adsorption					1214:1223	the adsorption	1210:1223	the adsorption of carbon and reduction of Fe0/Fe3O4 toward U	1210:1269	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	10	dep	synergistic	1280:1290	arg1	VI					1271:1272	VI	1271:1272	VI	1271:1272	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	4	11	from	interaction	647:657	arg1	process					707:713	the calcination process	691:713	the calcination process	691:713	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	5	12	theme	MHC-7	861:865	arg1	capacity					849:856	the maximum adsorption capacity	826:856	the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g)	826:888	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	12	theme	MHC-7	861:865	arg1	higher					908:913	higher	908:913	higher	908:913	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	7	13	theme	iron/carbon	1338:1348	arg1	composites					1350:1359	the iron/carbon composites	1334:1359	the iron/carbon composites	1334:1359	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	1	14	theme	iron/carbon	143:153	arg1	composites					155:164	Novel iron/carbon composites	137:164	Novel iron/carbon composites	137:164	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	0	15	theme	cellulose	82:90	arg1	coupling					70:77	the coupling	66:77	the coupling of cellulose with iron oxides	66:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	0	16	from	adsorption	16:25	arg1	composites					42:51	iron/carbon composites	30:51	iron/carbon composites derived from the coupling of cellulose with iron oxides	30:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	5	17	theme	Batch	764:768	arg1	experiments					770:780	Batch experiments	764:780	Batch experiments	764:780	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	3	18	theme	iron	531:534	arg1	precursors					536:545	iron precursors	531:545	iron precursors	531:545	The site of interaction between cellulose and iron precursors was illustrated (mainly combined with COO-).
31771849	0	19	theme	U	10:10	arg1	adsorption					16:25	Efficient U(VI) adsorption	0:25	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.	0:135	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	2	20	theme	various	349:355	arg1	techniques					357:366	various techniques	349:366	various techniques	349:366	Characterization by various techniques implied that chemical interaction between cellulose and Fe3O4/Fe0 existed in the as-prepared iron/carbon composites.
31771849	0	21	theme	Efficient	0:8	arg1	adsorption					16:25	Efficient U(VI) adsorption	0:25	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.	0:135	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	6	22	theme	iron/carbon	1182:1192	arg1	composites					1194:1203	iron/carbon composites	1182:1203	iron/carbon composites	1182:1203	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	23	theme	oxygen-containing	1101:1117	arg1	responsible					1131:1141	responsible	1131:1141	responsible	1131:1141	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	23	theme	oxygen-containing	1101:1117	arg1	groups					1119:1124	the oxygen-containing groups	1097:1124	the oxygen-containing groups	1097:1124	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	8	24	theme	iron/carbon	1669:1679	arg1	composites					1681:1690	iron/carbon composites	1669:1690	iron/carbon composites	1669:1690	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	7	25	theme	good	1373:1376	arg1	recyclability					1378:1390	a good recyclability	1371:1390	a good recyclability	1371:1390	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	8	26	from	adsorbents	1566:1575	arg1	cleanup					1594:1600	environmental cleanup	1580:1600	environmental cleanup	1580:1600	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	1	27	with	coupling	197:204	arg1	oxides					229:234	iron oxides	224:234	iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O)	224:274	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	0	28	with	coupling	70:77	arg1	oxides					102:107	iron oxides	97:107	iron oxides	97:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	5	29	theme	maximum	830:836	arg1	capacity					849:856	the maximum adsorption capacity	826:856	the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g)	826:888	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	29	theme	maximum	830:836	arg1	higher					908:913	higher	908:913	higher	908:913	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	7	30	from	recoverability	1393:1406	arg1	experiments					1463:1473	the regeneration experiments	1446:1473	the regeneration experiments	1446:1473	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	0	31	theme	iron	97:100	arg1	oxides					102:107	iron oxides	97:107	iron oxides	97:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	7	32	theme	regeneration	1450:1461	arg1	experiments					1463:1473	the regeneration experiments	1446:1473	the regeneration experiments	1446:1473	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	3	33	theme	interaction	497:507	arg1	interaction					497:507	interaction	497:507	interaction between cellulose and iron precursors	497:545	The site of interaction between cellulose and iron precursors was illustrated (mainly combined with COO-).
31771849	3	33	theme	interaction	497:507	arg1	site					489:492	The site	485:492	The site of interaction between cellulose and iron precursors	485:545	The site of interaction between cellulose and iron precursors was illustrated (mainly combined with COO-).
31771849	3	34	dep	combined	571:578	arg1	mainly					564:569	mainly	564:569	mainly	564:569	The site of interaction between cellulose and iron precursors was illustrated (mainly combined with COO-).
31771849	5	35	theme	adsorption	838:847	arg1	capacity					849:856	the maximum adsorption capacity	826:856	the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g)	826:888	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	35	theme	adsorption	838:847	arg1	higher					908:913	higher	908:913	higher	908:913	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	2	36	theme	iron/carbon	461:471	arg1	composites					473:482	the as-prepared iron/carbon composites	445:482	the as-prepared iron/carbon composites	445:482	Characterization by various techniques implied that chemical interaction between cellulose and Fe3O4/Fe0 existed in the as-prepared iron/carbon composites.
31771849	5	37	theme	remarkable	1014:1023	arg1	Fe2O3					985:989	Fe2O3	985:989	Fe2O3	985:989	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	37	theme	remarkable	1014:1023	arg1	resource					1030:1037	the remarkable iron resource	1010:1037	the remarkable iron resource for the iron/carbon composites	1010:1068	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	38	dep	U	871:871	arg1	VI					873:874	VI	873:874	VI	873:874	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	8	39	theme	composites	1681:1690	arg1	preparation					1654:1664	the preparation	1650:1664	the preparation of iron/carbon composites	1650:1690	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	2	40	theme	chemical	381:388	arg1	interaction					390:400	chemical interaction	381:400	chemical interaction between cellulose and Fe3O4/Fe0	381:432	Characterization by various techniques implied that chemical interaction between cellulose and Fe3O4/Fe0 existed in the as-prepared iron/carbon composites.
31771849	2	41	theme	as-prepared	449:459	arg1	composites					473:482	the as-prepared iron/carbon composites	445:482	the as-prepared iron/carbon composites	445:482	Characterization by various techniques implied that chemical interaction between cellulose and Fe3O4/Fe0 existed in the as-prepared iron/carbon composites.
31771849	7	42	dep	adsorption	1432:1441	arg1	VI					1428:1429	VI	1428:1429	VI	1428:1429	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	7	43	from	stability	1412:1420	arg1	experiments					1463:1473	the regeneration experiments	1446:1473	the regeneration experiments	1446:1473	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31771849	5	44	theme	iron	1025:1028	arg1	Fe2O3					985:989	Fe2O3	985:989	Fe2O3	985:989	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	5	44	theme	iron	1025:1028	arg1	resource					1030:1037	the remarkable iron resource	1010:1037	the remarkable iron resource for the iron/carbon composites	1010:1068	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	4	45	theme	Fe3+	614:617	arg1	self-reduction					596:609	The self-reduction	592:609	The self-reduction of Fe3+ to Fe2+ or even Fe0	592:637	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	4	45	theme	Fe3+	614:617	arg1	interaction					647:657	the interaction	643:657	the interaction between carbon and Fe3O4/Fe0 in the calcination process	643:713	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	8	46	theme	iron/carbon	1513:1523	arg1	adsorbents					1566:1575	valuable adsorbents	1557:1575	valuable adsorbents in environmental cleanup	1557:1600	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	8	46	theme	iron/carbon	1513:1523	arg1	composites					1525:1534	the iron/carbon composites	1509:1534	the iron/carbon composites	1509:1534	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	0	47	theme	iron/carbon	30:40	arg1	composites					42:51	iron/carbon composites	30:51	iron/carbon composites derived from the coupling of cellulose with iron oxides	30:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	6	48	theme	Fe0/Fe3O4	1252:1260	arg1	reduction					1239:1247	reduction	1239:1247	reduction	1239:1247	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	48	theme	Fe0/Fe3O4	1252:1260	arg1	carbon					1228:1233	carbon	1228:1233	carbon	1228:1233	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	0	49	dep	adsorption	16:25	arg1	mechanism					126:134	mechanism	126:134	mechanism	126:134	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	0	49	dep	adsorption	16:25	arg1	VI					12:13	VI	12:13	VI	12:13	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	0	49	dep	adsorption	16:25	arg1	Performance					110:120	Performance	110:120	Performance	110:120	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	0	50	attach	derived	53:59	arg2	composites					42:51	iron/carbon composites	30:51	iron/carbon composites derived from the coupling of cellulose with iron oxides	30:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	0	50	attach	derived	53:59	arg1	coupling					70:77	the coupling	66:77	the coupling of cellulose with iron oxides	66:107	Efficient U(VI) adsorption on iron/carbon composites derived from the coupling of cellulose with iron oxides: Performance and mechanism.
31771849	5	51	theme	spectroscopic	786:798	arg1	techniques					800:809	spectroscopic techniques	786:809	spectroscopic techniques	786:809	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	1	52	dep	α-FeOOH	242:248	arg1	e.g.					237:240	e.g.	237:240	e.g.	237:240	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	1	52	dep	α-FeOOH	242:248	arg1	3·9H2O					268:273	3·9H2O	268:273	3·9H2O	268:273	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	6	53	theme	reaction	1303:1310	arg1	process					1312:1318	the reaction process	1299:1318	the reaction process	1299:1318	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	54	theme	reduction	1239:1247	arg1	synergistic					1280:1290	synergistic	1280:1290	synergistic	1280:1290	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	54	theme	reduction	1239:1247	arg1	adsorption					1214:1223	the adsorption	1210:1223	the adsorption of carbon and reduction of Fe0/Fe3O4 toward U	1210:1269	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	54	theme	reduction	1239:1247	arg1	VI					1271:1272	VI	1271:1272	VI	1271:1272	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	55	theme	U	1173:1173	arg1	process					1162:1168	the adsorption process	1147:1168	the adsorption process of U	1147:1173	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	6	56	theme	XPS	1071:1073	arg1	results					1075:1081	XPS results	1071:1081	XPS results	1071:1081	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	1	57	theme	cellulose	209:217	arg1	coupling					197:204	coupling	197:204	coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O)	197:274	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	4	58	theme	composites	752:761	arg1	magnetism					735:743	the strong magnetism	724:743	the strong magnetism of the composites	724:761	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	4	59	theme	calcination	695:705	arg1	process					707:713	the calcination process	691:713	the calcination process	691:713	The self-reduction of Fe3+ to Fe2+ or even Fe0 and the interaction between carbon and Fe3O4/Fe0 in the calcination process realized the strong magnetism of the composites.
31771849	6	60	dep	composites	1194:1203	arg1	VI					1175:1176	VI	1175:1176	VI	1175:1176	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	8	61	theme	promising	1622:1630	arg1	resource					1637:1644	a promising iron resource	1620:1644	a promising iron resource for the preparation of iron/carbon composites	1620:1690	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	8	61	theme	promising	1622:1630	arg1	Fe2O3					1610:1614	the Fe2O3	1606:1614	the Fe2O3	1606:1614	These findings demonstrated that the iron/carbon composites can be considered as valuable adsorbents in environmental cleanup and the Fe2O3 was a promising iron resource for the preparation of iron/carbon composites.
31771849	3	62	dep	illustrated	551:561	arg1	combined					571:578	combined	571:578	combined with COO-	571:588	The site of interaction between cellulose and iron precursors was illustrated (mainly combined with COO-).
31771849	1	63	theme	iron	224:227	arg1	oxides					229:234	iron oxides	224:234	iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O)	224:274	Novel iron/carbon composites were successfully prepared via coupling of cellulose with iron oxides (e.g. α-FeOOH, Fe2O3 and Fe(NO3)3·9H2O) at different temperatures under nitrogen atmosphere.
31771849	6	64	theme	adsorption	1151:1160	arg1	process					1162:1168	the adsorption process	1147:1168	the adsorption process of U	1147:1173	XPS results revealed that the oxygen-containing groups were responsible for the adsorption process of U(VI) on iron/carbon composites, and the adsorption of carbon and reduction of Fe0/Fe3O4 toward U(VI) were synergistic during the reaction process.
31771849	5	65	theme	iron/carbon	1047:1057	arg1	composites					1059:1068	the iron/carbon composites	1043:1068	the iron/carbon composites	1043:1068	Batch experiments and spectroscopic techniques indicated that the maximum adsorption capacity of MHC-7 for U(VI) (105.3 mg/g) was significantly higher than that of MGC-7 (86.0 mg/g) and MFC-7 (79.0 mg/g), indicating that Fe2O3 can be regarded as the remarkable iron resource for the iron/carbon composites.
31771849	7	66	from	recyclability	1378:1390	arg1	experiments					1463:1473	the regeneration experiments	1446:1473	the regeneration experiments	1446:1473	In addition, the iron/carbon composites exhibited a good recyclability, recoverability and stability for U(VI) adsorption in the regeneration experiments.
31514047	1	0	theme	physicochemical	141:155	arg1	properties					157:166	The physicochemical properties	137:166	The physicochemical properties of wheat bran	137:180	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	0	1	theme	physicochemical	72:86	arg1	properties					88:97	the physicochemical properties	68:97	the physicochemical properties of wheat (Triticum aestivum L.) bran	68:134	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	5	2	theme	increased	909:917	arg1	extractability					919:932	an increased extractability	906:932	an increased extractability of starch and arabinoxylan	906:959	This coincided with an increased extractability of starch and arabinoxylan.
31514047	0	3	theme	wheat	102:106	arg1	bran					131:134	wheat (Triticum aestivum L.) bran	102:134	wheat (Triticum aestivum L.) bran	102:134	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	0	3	theme	wheat	102:106	arg1	Triticum					109:116	Triticum	109:116	Triticum	109:116	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	4	4	theme	median	791:796	arg1	size					807:810	wheat bran median particle size	780:810	wheat bran median particle size	780:810	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	6	5	theme	2	1230:1230	arg1	%					1231:1231	%	1231:1231	%	1231:1231	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	0	6	dep	Triticum	109:116	arg1	L.					127:128	Triticum aestivum L.	109:128	Triticum aestivum L.	109:128	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	6	7	theme	higher	1153:1158	arg1	WRC					1160:1162	higher WRC	1153:1162	higher WRC	1153:1162	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	6	8	theme	minor	1001:1005	arg1	importance					1007:1016	minor importance	1001:1016	minor importance	1001:1016	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	9	theme	wheat	571:575	arg1	bran					577:580	wheat bran	571:580	wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation)	571:711	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	10	theme	smaller	1130:1136	arg1	size					1147:1150	a smaller particle size	1128:1150	a smaller particle size	1128:1150	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	11	theme	I-optimal	361:369	arg1	design					384:389	An I-optimal experimental design	358:389	An I-optimal experimental design	358:389	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	2	12	theme	physicochemical	272:286	arg1	modifications					288:300	physicochemical modifications	272:300	physicochemical modifications	272:300	The possibility to induce physicochemical modifications in wheat bran using microfluidisation was investigated.
31514047	3	13	theme	parameters	458:467	arg1	effect					419:424	the effect	415:424	the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation)	415:711	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	14	theme	experimental	371:382	arg1	design					384:389	An I-optimal experimental design	358:389	An I-optimal experimental design	358:389	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	15	theme	microfluidisation	429:445	arg1	pressure					470:477	pressure	470:477	pressure	470:477	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	15	theme	microfluidisation	429:445	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	15	theme	microfluidisation	429:445	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	15	theme	microfluidisation	429:445	arg1	concentration					503:515	bran concentration	498:515	bran concentration	498:515	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	15	theme	microfluidisation	429:445	arg1	number					480:485	number	480:485	number of passes	480:495	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	4	16	theme	wheat	780:784	arg1	bran					786:789	wheat bran	780:789	wheat bran median particle size	780:810	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	1	17	theme	wheat	171:175	arg1	bran					177:180	wheat bran	171:180	wheat bran	171:180	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	6	18	theme	particle	980:987	arg1	size					989:992	the initial particle size	968:992	the initial particle size	968:992	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	4	19	theme	attached	866:873	arg1	endosperm					875:883	the attached endosperm	862:883	the attached endosperm	862:883	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	3	20	theme	particle	583:590	arg1	size					592:595	particle size	583:595	particle size	583:595	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	21	theme	important	547:555	arg1	properties					557:566	important properties	547:566	important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation)	547:711	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	1	22	theme	bran	177:180	arg1	properties					157:166	The physicochemical properties	137:166	The physicochemical properties of wheat bran	137:180	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	6	23	theme	bran	1239:1242	arg1	suspension					1244:1253	a 2% wheat bran suspension	1228:1253	a 2% wheat bran suspension	1228:1253	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	6	24	theme	lower	1066:1070	arg1	concentration					1077:1089	lower bran concentration	1066:1089	lower bran concentration during microfluidisation	1066:1114	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	25	theme	bran	577:580	arg1	properties					557:566	important properties	547:566	important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation)	547:711	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	26	theme	wheat	1233:1237	arg1	bran					1239:1242	a 2% wheat bran	1228:1242	a 2% wheat bran suspension	1228:1253	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	27	theme	passes	490:495	arg1	pressure					470:477	pressure	470:477	pressure	470:477	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	27	theme	passes	490:495	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	27	theme	passes	490:495	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	27	theme	passes	490:495	arg1	concentration					503:515	bran concentration	498:515	bran concentration	498:515	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	27	theme	passes	490:495	arg1	number					480:485	number	480:485	number of passes	480:495	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	28	theme	increased	1191:1199	arg1	viscosity					1201:1209	an increased viscosity	1188:1209	an increased viscosity	1188:1209	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	29	used	used	395:398	arg2	design					384:389	An I-optimal experimental design	358:389	An I-optimal experimental design	358:389	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	1	30	contain	have	182:185	arg2	effect					190:195	an effect	187:195	an effect	187:195	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	1	30	contain	have	182:185	arg1	properties					157:166	The physicochemical properties	137:166	The physicochemical properties of wheat bran	137:180	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	2	31	theme	wheat	305:309	arg1	bran					311:314	wheat bran	305:314	wheat bran using microfluidisation	305:338	The possibility to induce physicochemical modifications in wheat bran using microfluidisation was investigated.
31514047	6	32	theme	passes	1055:1060	arg1	pressure					1028:1035	a higher pressure	1019:1035	a higher pressure	1019:1035	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	6	32	theme	passes	1055:1060	arg1	number					1045:1050	larger number	1038:1050	larger number of passes	1038:1060	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	6	32	theme	passes	1055:1060	arg1	concentration					1077:1089	lower bran concentration	1066:1089	lower bran concentration during microfluidisation	1066:1114	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	0	33	theme	microfluidisation	25:41	arg1	parameters					54:63	microfluidisation processing parameters	25:63	microfluidisation processing parameters	25:63	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	5	34	theme	starch	937:942	arg1	extractability					919:932	an increased extractability	906:932	an increased extractability of starch and arabinoxylan	906:959	This coincided with an increased extractability of starch and arabinoxylan.
31514047	0	35	theme	bran	131:134	arg1	properties					88:97	the physicochemical properties	68:97	the physicochemical properties of wheat (Triticum aestivum L.) bran	68:134	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	3	36	dep	parameters	458:467	arg1	pressure					470:477	pressure	470:477	pressure	470:477	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	36	dep	parameters	458:467	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	36	dep	parameters	458:467	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	36	dep	parameters	458:467	arg1	concentration					503:515	bran concentration	498:515	bran concentration	498:515	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	36	dep	parameters	458:467	arg1	number					480:485	number	480:485	number of passes	480:495	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	37	theme	processing	447:456	arg1	pressure					470:477	pressure	470:477	pressure	470:477	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	37	theme	processing	447:456	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	37	theme	processing	447:456	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	37	theme	processing	447:456	arg1	concentration					503:515	bran concentration	498:515	bran concentration	498:515	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	37	theme	processing	447:456	arg1	number					480:485	number	480:485	number of passes	480:495	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	38	theme	initial	972:978	arg1	size					989:992	the initial particle size	968:992	the initial particle size	968:992	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	39	theme	chemical	614:621	arg1	composition					623:633	chemical composition	614:633	chemical composition	614:633	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	40	theme	bran	1072:1075	arg1	concentration					1077:1089	lower bran concentration	1066:1089	lower bran concentration during microfluidisation	1066:1114	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	0	41	theme	parameters	54:63	arg1	effect					15:20	the effect	11:20	the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran	11:134	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	3	42	theme	particle	529:536	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	42	theme	particle	529:536	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	4	43	theme	starch	841:846	arg1	granules					848:855	starch granules	841:855	starch granules	841:855	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	0	44	theme	processing	43:52	arg1	parameters					54:63	microfluidisation processing parameters	25:63	microfluidisation processing parameters	25:63	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	3	45	from	effect	419:424	arg1	properties					557:566	important properties	547:566	important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation)	547:711	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	46	theme	initial	521:527	arg1	size					538:541	initial particle size	521:541	initial particle size	521:541	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	46	theme	initial	521:527	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	4	47	theme	particle	798:805	arg1	size					807:810	wheat bran median particle size	780:810	wheat bran median particle size	780:810	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	3	48	theme	water	636:640	arg1	WRC					662:664	WRC	662:664	WRC	662:664	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	48	theme	water	636:640	arg1	capacity					652:659	water retention capacity	636:659	water retention capacity (WRC)	636:665	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	6	49	theme	%	1231:1231	arg1	bran					1239:1242	a 2% wheat bran	1228:1242	a 2% wheat bran suspension	1228:1253	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	50	theme	bran	498:501	arg1	parameters					458:467	microfluidisation processing parameters	429:467	microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size)	429:542	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	50	theme	bran	498:501	arg1	concentration					503:515	bran concentration	498:515	bran concentration	498:515	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	1	51	theme	technofunctional	204:219	arg1	profile					237:243	its technofunctional and nutritional profile	200:243	its technofunctional and nutritional profile	200:243	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	4	52	theme	bran	786:789	arg1	size					807:810	wheat bran median particle size	780:810	wheat bran median particle size	780:810	With the parameters used in this study, microfluidisation reduced wheat bran median particle size to 14.8 μm and disintegrated starch granules from the attached endosperm.
31514047	3	53	theme	retention	642:650	arg1	WRC					662:664	WRC	662:664	WRC	662:664	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	53	theme	retention	642:650	arg1	capacity					652:659	water retention capacity	636:659	water retention capacity (WRC)	636:665	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	5	54	theme	arabinoxylan	948:959	arg1	extractability					919:932	an increased extractability	906:932	an increased extractability of starch and arabinoxylan	906:959	This coincided with an increased extractability of starch and arabinoxylan.
31514047	6	55	theme	particle	1138:1145	arg1	size					1147:1150	a smaller particle size	1128:1150	a smaller particle size	1128:1150	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	3	56	dep	bran	577:580	arg1	capacity					652:659	water retention capacity	636:659	water retention capacity (WRC)	636:665	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	WRC					662:664	WRC	662:664	WRC	662:664	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	sedimentation					698:710	sedimentation	698:710	sedimentation	698:710	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	composition					623:633	chemical composition	614:633	chemical composition	614:633	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	size					592:595	particle size	583:595	particle size	583:595	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	extractability					668:681	extractability	668:681	extractability	668:681	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	microstructure					598:611	microstructure	598:611	microstructure	598:611	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	3	56	dep	bran	577:580	arg1	viscosity					684:692	viscosity	684:692	viscosity	684:692	An I-optimal experimental design was used to investigate the effect of microfluidisation processing parameters (pressure, number of passes, bran concentration and initial particle size) on important properties of wheat bran (particle size, microstructure, chemical composition, water retention capacity (WRC), extractability, viscosity and sedimentation).
31514047	1	57	theme	nutritional	225:235	arg1	profile					237:243	its technofunctional and nutritional profile	200:243	its technofunctional and nutritional profile	200:243	The physicochemical properties of wheat bran have an effect on its technofunctional and nutritional profile.
31514047	0	58	from	effect	15:20	arg1	properties					88:97	the physicochemical properties	68:97	the physicochemical properties of wheat (Triticum aestivum L.) bran	68:134	Study into the effect of microfluidisation processing parameters on the physicochemical properties of wheat (Triticum aestivum L.) bran.
31514047	6	59	theme	larger	1038:1043	arg1	number					1045:1050	larger number	1038:1050	larger number of passes	1038:1060	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31514047	6	60	theme	higher	1021:1026	arg1	pressure					1028:1035	a higher pressure	1019:1035	a higher pressure	1019:1035	While the initial particle size was of minor importance, a higher pressure, larger number of passes and lower bran concentration during microfluidisation resulted in a smaller particle size, higher WRC and extractability, and an increased viscosity and stability in a 2% wheat bran suspension.
31070898	2	0	from	growth	428:433	arg1	patterns					503:510	these patterns	497:510	these patterns	497:510	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	1	theme	fibrin	372:377	arg1	time					388:391	the fibrin clotting time	368:391	the fibrin clotting time of human blood plasma	368:413	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	6	2	attach	present	1116:1122	arg2	cellulose					1106:1114	cellulose	1106:1114	cellulose present in the films	1106:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	2	attach	present	1116:1122	arg1	films					1131:1135	the films	1127:1135	the films	1127:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	3	3	theme	polycaprolactone	544:559	arg1	blends					534:539	blends	534:539	blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose	534:604	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	1	4	theme	micro-	275:280	arg1	interfaces					308:317	micro- and nanopatterned polymer interfaces	275:317	micro- and nanopatterned polymer interfaces	275:317	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	9	5	theme	composite	1566:1574	arg1	surfaces					1576:1583	presented patterned polymer composite surfaces	1538:1583	presented patterned polymer composite surfaces	1538:1583	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	4	6	theme	hydrophobic	880:890	arg1	PCL					892:894	hydrophobic PCL	880:894	hydrophobic PCL	880:894	Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL.
31070898	3	7	theme	spinning	710:717	arg1	solutions					719:727	the spinning solutions	706:727	the spinning solutions	706:727	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	7	8	theme	fibrin	1225:1230	arg1	times					1241:1245	significantly increased fibrin clotting times	1201:1245	significantly increased fibrin clotting times	1201:1245	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	5	9	theme	blood	901:905	arg1	proteins					914:921	The blood plasma proteins	897:921	The blood plasma proteins	897:921	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	2	10	theme	adsorption	328:337	arg1	studies					339:345	Protein adsorption studies	320:345	Protein adsorption studies	320:345	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	10	11	theme	vascular	1799:1806	arg1	grafts					1808:1813	artificial vascular grafts	1788:1813	artificial vascular grafts	1788:1813	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	1	12	theme	blood	211:215	arg1	fibrinogen					242:251	fibrinogen	242:251	fibrinogen	242:251	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	12	theme	blood	211:215	arg1	γ-globulins					258:268	γ-globulins	258:268	γ-globulins	258:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	12	theme	blood	211:215	arg1	albumin					233:239	albumin	233:239	albumin	233:239	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	12	theme	blood	211:215	arg1	proteins					224:231	the human blood plasma proteins	201:231	the human blood plasma proteins albumin, fibrinogen, and γ-globulins	201:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	7	13	from	times	1241:1245	arg1	films					1307:1311	cellulose-containing films	1286:1311	cellulose-containing films	1286:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	0	14	theme	Cellular	143:150	arg1	Response					152:159	Endothelial Cellular Response	131:159	Endothelial Cellular Response	131:159	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	3	15	theme	various	611:617	arg1	patterns					629:636	various thin-film patterns	611:636	various thin-film patterns	611:636	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	1	16	theme	proteins	224:231	arg1	interaction					186:196	the interaction	182:196	the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces	182:317	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	3	17	theme	cellulose	596:604	arg1	blends					534:539	blends	534:539	blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose	534:604	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	4	18	theme	Vapor-phase	730:740	arg1	deprotection					757:768	Vapor-phase acid-catalyzed deprotection	730:768	Vapor-phase acid-catalyzed deprotection	730:768	Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL.
31070898	10	19	used	utilized	1734:1741	arg2	knowledge					1717:1725	this knowledge	1712:1725	this knowledge	1712:1725	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	3	20	theme	spin	645:648	arg1	coating					650:656	spin coating	645:656	spin coating	645:656	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	7	21	from	clots	1277:1281	arg1	films					1307:1311	cellulose-containing films	1286:1311	cellulose-containing films	1286:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	8	22	theme	Cell	1387:1390	arg1	viability					1392:1400	Cell viability	1387:1400	Cell viability of hECs grown on these surfaces	1387:1432	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
31070898	7	23	theme	clots	1277:1281	arg1	times					1241:1245	significantly increased fibrin clotting times	1201:1245	significantly increased fibrin clotting times	1201:1245	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	7	23	theme	clots	1277:1281	arg1	masses					1257:1262	lower masses	1251:1262	lower masses of deposited clots on cellulose-containing films	1251:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	9	24	theme	controllable	1597:1608	arg1	coagulation					1631:1641	a controllable blood plasma protein coagulation	1595:1641	a controllable blood plasma protein coagulation	1595:1641	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	6	25	theme	inverse	1039:1045	arg1	correlation					1060:1070	An inverse proportional correlation	1036:1070	An inverse proportional correlation	1036:1070	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	9	26	theme	plasma	1616:1621	arg1	coagulation					1631:1641	a controllable blood plasma protein coagulation	1595:1641	a controllable blood plasma protein coagulation	1595:1641	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	0	27	theme	Fibrin	104:109	arg1	Formation					116:124	Fibrin Clot Formation	104:124	Fibrin Clot Formation	104:124	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	6	28	theme	present	1116:1122	arg1	cellulose					1106:1114	cellulose	1106:1114	cellulose present in the films	1106:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	2	29	theme	artery	462:467	arg1	cells					481:485	primary human pulmonary artery endothelial cells	438:485	primary human pulmonary artery endothelial cells (hECs)	438:492	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	29	theme	artery	462:467	arg1	hECs					488:491	hECs	488:491	hECs	488:491	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	0	30	theme	Protein	84:90	arg1	Adsorption					92:101	Tunable Protein Adsorption	76:101	Tunable Protein Adsorption	76:101	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	2	31	theme	blood	402:406	arg1	plasma					408:413	human blood plasma	396:413	human blood plasma	396:413	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	0	32	theme	Nano-	0:4	arg1	Surfaces					62:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces	0:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.	0:160	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	9	33	theme	biological	1661:1670	arg1	response					1672:1679	a significant biological response	1647:1679	a significant biological response from hECs	1647:1689	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	2	34	theme	human	446:450	arg1	cells					481:485	primary human pulmonary artery endothelial cells	438:485	primary human pulmonary artery endothelial cells (hECs)	438:492	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	34	theme	human	446:450	arg1	hECs					488:491	hECs	488:491	hECs	488:491	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	8	35	theme	higher	1463:1468	arg1	adsorption					1478:1487	higher protein adsorption	1463:1487	higher protein adsorption	1463:1487	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
31070898	10	36	theme	cell	1769:1772	arg1	cultures					1774:1781	cell cultures	1769:1781	cell cultures	1769:1781	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	0	37	theme	Micropatterned	10:23	arg1	Surfaces					62:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces	0:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.	0:160	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	1	38	theme	polymer	300:306	arg1	interfaces					308:317	micro- and nanopatterned polymer interfaces	275:317	micro- and nanopatterned polymer interfaces	275:317	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	5	39	theme	selective	987:995	arg1	deposition					1005:1014	a suggested selective protein deposition	975:1014	a suggested selective protein deposition on the PCL domains	975:1033	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	9	40	from	hECs	1686:1689	arg1	response					1672:1679	a significant biological response	1647:1679	a significant biological response from hECs	1647:1689	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	9	40	from	hECs	1686:1689	arg1	coagulation					1631:1641	a controllable blood plasma protein coagulation	1595:1641	a controllable blood plasma protein coagulation	1595:1641	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	4	41	theme	cellulose	850:858	arg1	domains					860:866	hydrophilic cellulose domains	838:866	hydrophilic cellulose domains enclosed by hydrophobic PCL	838:894	Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL.
31070898	0	42	theme	Cellulose	42:50	arg1	Surfaces					62:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces	0:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.	0:160	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	2	43	theme	cells	481:485	arg1	growth					428:433	the growth	424:433	the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns	424:510	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	0	44	theme	Tunable	76:82	arg1	Adsorption					92:101	Tunable Protein Adsorption	76:101	Tunable Protein Adsorption	76:101	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	10	45	theme	artificial	1788:1797	arg1	grafts					1808:1813	artificial vascular grafts	1788:1813	artificial vascular grafts	1788:1813	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	7	46	theme	crystal	1335:1341	arg1	microbalance					1343:1354	quartz crystal microbalance	1328:1354	quartz crystal microbalance with dissipation measurements	1328:1384	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	8	47	theme	faster	1493:1498	arg1	formation					1505:1513	faster clot formation	1493:1513	faster clot formation	1493:1513	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
31070898	1	48	with	interaction	186:196	arg1	interfaces					308:317	micro- and nanopatterned polymer interfaces	275:317	micro- and nanopatterned polymer interfaces	275:317	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	3	49	theme	mass	676:679	arg1	ratio					681:685	the mass ratio	672:685	the mass ratio of the polymers in the spinning solutions	672:727	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	0	50	with	Surfaces	62:69	arg1	Adsorption					92:101	Tunable Protein Adsorption	76:101	Tunable Protein Adsorption	76:101	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	0	50	with	Surfaces	62:69	arg1	Response					152:159	Endothelial Cellular Response	131:159	Endothelial Cellular Response	131:159	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	0	50	with	Surfaces	62:69	arg1	Formation					116:124	Fibrin Clot Formation	104:124	Fibrin Clot Formation	104:124	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	10	51	theme	careful	1820:1826	arg1	choice					1828:1833	a careful choice	1818:1833	a careful choice of polymers and patterns	1818:1858	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	3	52	from	ratio	681:685	arg1	solutions					719:727	the spinning solutions	706:727	the spinning solutions	706:727	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	2	53	theme	clotting	379:386	arg1	time					388:391	the fibrin clotting time	368:391	the fibrin clotting time of human blood plasma	368:413	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	7	54	theme	cellulose-containing	1286:1305	arg1	films					1307:1311	cellulose-containing films	1286:1311	cellulose-containing films	1286:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	3	55	theme	polymers	694:701	arg1	ratio					681:685	the mass ratio	672:685	the mass ratio of the polymers in the spinning solutions	672:727	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	10	56	theme	polymers	1838:1845	arg1	choice					1828:1833	a careful choice	1818:1833	a careful choice of polymers and patterns	1818:1858	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	5	57	from	deposition	1005:1014	arg1	domains					1027:1033	the PCL domains	1019:1033	the PCL domains	1019:1033	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	7	58	from	films	1307:1311	arg1	times					1241:1245	significantly increased fibrin clotting times	1201:1245	significantly increased fibrin clotting times	1201:1245	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	7	58	from	films	1307:1311	arg1	masses					1257:1262	lower masses	1251:1262	lower masses of deposited clots on cellulose-containing films	1251:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	6	59	theme	adsorbed	1166:1173	arg1	proteins					1175:1182	irreversibly adsorbed proteins	1153:1182	irreversibly adsorbed proteins	1153:1182	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	60	from	present	1116:1122	arg1	films					1131:1135	the films	1127:1135	the films	1127:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	5	61	theme	plasma	907:912	arg1	proteins					914:921	The blood plasma proteins	897:921	The blood plasma proteins	897:921	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	0	62	theme	Endothelial	131:141	arg1	Response					152:159	Endothelial Cellular Response	131:159	Endothelial Cellular Response	131:159	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	2	63	theme	Protein	320:326	arg1	studies					339:345	Protein adsorption studies	320:345	Protein adsorption studies	320:345	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	1	64	theme	human	205:209	arg1	fibrinogen					242:251	fibrinogen	242:251	fibrinogen	242:251	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	64	theme	human	205:209	arg1	γ-globulins					258:268	γ-globulins	258:268	γ-globulins	258:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	64	theme	human	205:209	arg1	albumin					233:239	albumin	233:239	albumin	233:239	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	64	theme	human	205:209	arg1	proteins					224:231	the human blood plasma proteins	201:231	the human blood plasma proteins albumin, fibrinogen, and γ-globulins	201:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	6	65	theme	proteins	1175:1182	arg1	cellulose					1106:1114	cellulose	1106:1114	cellulose present in the films	1106:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	65	theme	proteins	1175:1182	arg1	proteins					1175:1182	irreversibly adsorbed proteins	1153:1182	irreversibly adsorbed proteins	1153:1182	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	65	theme	proteins	1175:1182	arg1	amount					1096:1101	the amount	1092:1101	the amount of cellulose present in the films	1092:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	65	theme	proteins	1175:1182	arg1	mass					1145:1148	the mass	1141:1148	the mass of irreversibly adsorbed proteins	1141:1182	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	1	66	theme	plasma	217:222	arg1	fibrinogen					242:251	fibrinogen	242:251	fibrinogen	242:251	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	66	theme	plasma	217:222	arg1	γ-globulins					258:268	γ-globulins	258:268	γ-globulins	258:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	66	theme	plasma	217:222	arg1	albumin					233:239	albumin	233:239	albumin	233:239	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	66	theme	plasma	217:222	arg1	proteins					224:231	the human blood plasma proteins	201:231	the human blood plasma proteins albumin, fibrinogen, and γ-globulins	201:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	3	67	theme	trimethylsilyl-protected	571:594	arg1	cellulose					596:604	trimethylsilyl-protected cellulose	571:604	trimethylsilyl-protected cellulose	571:604	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	5	68	theme	cellulose	943:951	arg1	domains					953:959	the cellulose domains	939:959	the cellulose domains	939:959	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	7	69	with	microbalance	1343:1354	arg1	measurements					1373:1384	dissipation measurements	1361:1384	dissipation measurements	1361:1384	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	7	70	from	masses	1257:1262	arg1	films					1307:1311	cellulose-containing films	1286:1311	cellulose-containing films	1286:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	10	71	theme	patterns	1851:1858	arg1	choice					1828:1833	a careful choice	1818:1833	a careful choice of polymers and patterns	1818:1858	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	9	72	theme	protein	1623:1629	arg1	coagulation					1631:1641	a controllable blood plasma protein coagulation	1595:1641	a controllable blood plasma protein coagulation	1595:1641	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	7	73	theme	deposited	1267:1275	arg1	clots					1277:1281	deposited clots	1267:1281	deposited clots on cellulose-containing films	1267:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	3	74	theme	thin-film	619:627	arg1	patterns					629:636	various thin-film patterns	611:636	various thin-film patterns	611:636	It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions.
31070898	6	75	theme	proportional	1047:1058	arg1	correlation					1060:1070	An inverse proportional correlation	1036:1070	An inverse proportional correlation	1036:1070	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	7	76	theme	lower	1251:1255	arg1	masses					1257:1262	lower masses	1251:1262	lower masses of deposited clots on cellulose-containing films	1251:1311	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	9	77	theme	blood	1610:1614	arg1	coagulation					1631:1641	a controllable blood plasma protein coagulation	1595:1641	a controllable blood plasma protein coagulation	1595:1641	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	0	78	theme	Clot	111:114	arg1	Formation					116:124	Fibrin Clot Formation	104:124	Fibrin Clot Formation	104:124	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	5	79	theme	protein	997:1003	arg1	deposition					1005:1014	a suggested selective protein deposition	975:1014	a suggested selective protein deposition on the PCL domains	975:1033	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	8	80	theme	hECs	1405:1408	arg1	viability					1392:1400	Cell viability	1387:1400	Cell viability of hECs grown on these surfaces	1387:1432	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
31070898	7	81	theme	clotting	1232:1239	arg1	times					1241:1245	significantly increased fibrin clotting times	1201:1245	significantly increased fibrin clotting times	1201:1245	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	9	82	theme	presented	1538:1546	arg1	surfaces					1576:1583	presented patterned polymer composite surfaces	1538:1583	presented patterned polymer composite surfaces	1538:1583	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	2	83	theme	plasma	408:413	arg1	time					388:391	the fibrin clotting time	368:391	the fibrin clotting time of human blood plasma	368:413	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	7	84	theme	increased	1215:1223	arg1	times					1241:1245	significantly increased fibrin clotting times	1201:1245	significantly increased fibrin clotting times	1201:1245	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	9	85	theme	significant	1649:1659	arg1	response					1672:1679	a significant biological response	1647:1679	a significant biological response from hECs	1647:1689	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	6	86	theme	cellulose	1106:1114	arg1	cellulose					1106:1114	cellulose	1106:1114	cellulose present in the films	1106:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	86	theme	cellulose	1106:1114	arg1	proteins					1175:1182	irreversibly adsorbed proteins	1153:1182	irreversibly adsorbed proteins	1153:1182	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	86	theme	cellulose	1106:1114	arg1	amount					1096:1101	the amount	1092:1101	the amount of cellulose present in the films	1092:1135	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	6	86	theme	cellulose	1106:1114	arg1	mass					1145:1148	the mass	1141:1148	the mass of irreversibly adsorbed proteins	1141:1182	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	2	87	theme	pulmonary	452:460	arg1	cells					481:485	primary human pulmonary artery endothelial cells	438:485	primary human pulmonary artery endothelial cells (hECs)	438:492	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	87	theme	pulmonary	452:460	arg1	hECs					488:491	hECs	488:491	hECs	488:491	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	4	88	theme	acid-catalyzed	742:755	arg1	deprotection					757:768	Vapor-phase acid-catalyzed deprotection	730:768	Vapor-phase acid-catalyzed deprotection	730:768	Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL.
31070898	2	89	theme	human	396:400	arg1	plasma					408:413	human blood plasma	396:413	human blood plasma	396:413	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	0	90	theme	Polycaprolactone	25:40	arg1	Surfaces					62:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces	0:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.	0:160	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	1	91	theme	nanopatterned	286:298	arg1	interfaces					308:317	micro- and nanopatterned polymer interfaces	275:317	micro- and nanopatterned polymer interfaces	275:317	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	5	92	theme	suggested	977:985	arg1	deposition					1005:1014	a suggested selective protein deposition	975:1014	a suggested selective protein deposition on the PCL domains	975:1033	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	7	93	theme	dissipation	1361:1371	arg1	measurements					1373:1384	dissipation measurements	1361:1384	dissipation measurements	1361:1384	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	2	94	theme	primary	438:444	arg1	cells					481:485	primary human pulmonary artery endothelial cells	438:485	primary human pulmonary artery endothelial cells (hECs)	438:492	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	94	theme	primary	438:444	arg1	hECs					488:491	hECs	488:491	hECs	488:491	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	0	95	theme	Composite	52:60	arg1	Surfaces					62:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces	0:69	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.	0:160	Nano- and Micropatterned Polycaprolactone Cellulose Composite Surfaces with Tunable Protein Adsorption, Fibrin Clot Formation, and Endothelial Cellular Response.
31070898	9	96	theme	patterned	1548:1556	arg1	surfaces					1576:1583	presented patterned polymer composite surfaces	1538:1583	presented patterned polymer composite surfaces	1538:1583	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	4	97	theme	hydrophilic	838:848	arg1	domains					860:866	hydrophilic cellulose domains	838:866	hydrophilic cellulose domains enclosed by hydrophobic PCL	838:894	Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL.
31070898	10	98	theme	regenerative	1746:1757	arg1	medicine					1759:1766	regenerative medicine	1746:1766	regenerative medicine	1746:1766	It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns.
31070898	7	99	theme	quartz	1328:1333	arg1	microbalance					1343:1354	quartz crystal microbalance	1328:1354	quartz crystal microbalance with dissipation measurements	1328:1384	This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements.
31070898	2	100	theme	endothelial	469:479	arg1	cells					481:485	primary human pulmonary artery endothelial cells	438:485	primary human pulmonary artery endothelial cells (hECs)	438:492	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	2	100	theme	endothelial	469:479	arg1	hECs					488:491	hECs	488:491	hECs	488:491	Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns.
31070898	8	101	theme	protein	1470:1476	arg1	adsorption					1478:1487	higher protein adsorption	1463:1487	higher protein adsorption	1463:1487	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
31070898	5	102	theme	PCL	1023:1025	arg1	domains					1027:1033	the PCL domains	1019:1033	the PCL domains	1019:1033	The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains.
31070898	1	103	dep	proteins	224:231	arg1	fibrinogen					242:251	fibrinogen	242:251	fibrinogen	242:251	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	103	dep	proteins	224:231	arg1	γ-globulins					258:268	γ-globulins	258:268	γ-globulins	258:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	103	dep	proteins	224:231	arg1	albumin					233:239	albumin	233:239	albumin	233:239	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	1	103	dep	proteins	224:231	arg1	proteins					224:231	the human blood plasma proteins	201:231	the human blood plasma proteins albumin, fibrinogen, and γ-globulins	201:268	This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces.
31070898	6	104	from	films	1131:1135	arg1	present					1116:1122	present	1116:1122	present	1116:1122	An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins.
31070898	9	105	theme	polymer	1558:1564	arg1	surfaces					1576:1583	presented patterned polymer composite surfaces	1538:1583	presented patterned polymer composite surfaces	1538:1583	The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs.
31070898	8	106	theme	clot	1500:1503	arg1	formation					1505:1513	faster clot formation	1493:1513	faster clot formation	1493:1513	Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation.
29571131	3	0	theme	ions	722:725	arg1	analysis					696:703	simultaneous analysis	683:703	simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content	683:791	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	4	1	theme	parameters	815:824	arg1	control					798:804	NMR control	794:804	NMR control of these parameters	794:824	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	5	2	theme	Validation	1020:1029	arg1	results					1031:1037	Validation results	1020:1037	Validation results in terms of precision, reproducibility, limit of detection and recovery	1020:1109	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	6	3	theme	representative	1232:1245	arg1	Na-					1272:1274	more than hundred Na-	1254:1274	more than hundred Na-	1254:1274	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	3	theme	representative	1232:1245	arg1	set					1247:1249	a representative set	1230:1249	a representative set of more than hundred Na-	1230:1274	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	3	4	theme	water	779:783	arg1	content					785:791	water content	779:791	water content	779:791	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	2	5	theme	simultaneous	461:472	arg1	quantification					474:487	the simultaneous quantification	457:487	the simultaneous quantification of inorganic compounds (water, anions, cations)	457:535	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	2	6	from	lack	437:440	arg1	matrix					545:550	this matrix	540:550	this matrix	540:550	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	7	7	theme	analytical	1447:1456	arg1	method					1458:1463	a unique analytical method	1438:1463	a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin	1438:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	5	8	theme	authentic	1177:1185	arg1	samples					1195:1201	the authentic heparin samples	1173:1201	the authentic heparin samples	1173:1201	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	3	9	theme	content	785:791	arg1	analysis					696:703	simultaneous analysis	683:703	simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content	683:791	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	6	10	theme	Ca-	1280:1282	arg1	heparin					1284:1290	Ca- heparin	1280:1290	Ca- heparin	1280:1290	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	4	11	dep	1H	948:949	arg1	23Na					956:959	23Na	956:959	23Na	956:959	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	4	11	dep	1H	948:949	arg1	35Cl					966:969	35Cl	966:969	35Cl	966:969	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	4	11	dep	1H	948:949	arg1	2D					952:953	2D	952:953	2D	952:953	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	5	12	theme	heparin	1187:1193	arg1	samples					1195:1201	the authentic heparin samples	1173:1201	the authentic heparin samples	1173:1201	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	1	13	theme	disaccharide	228:239	arg1	units					241:245	disaccharide units	228:245	disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine	228:309	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	0	14	theme	water	83:87	arg1	analysis					71:78	the quantitative analysis	54:78	the quantitative analysis of water	54:87	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	4	15	theme	sequential	920:929	arg1	experiments					935:945	just four sequential NMR experiments	910:945	just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min	910:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	7	16	theme	quantitative	1495:1506	arg1	control					1508:1514	the simultaneous quantitative control	1478:1514	the simultaneous quantitative control of organic and inorganic composition of heparin	1478:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	6	17	theme	Na-	1272:1274	arg1	Na-					1272:1274	more than hundred Na-	1254:1274	more than hundred Na-	1254:1274	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	17	theme	Na-	1272:1274	arg1	set					1247:1249	a representative set	1230:1249	a representative set of more than hundred Na-	1230:1274	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	0	18	from	tool	45:48	arg1	pharmaceuticals					101:115	pharmaceuticals	101:115	pharmaceuticals	101:115	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	4	19	theme	NMR	794:796	arg1	control					798:804	NMR control	794:804	NMR control of these parameters	794:824	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	6	20	theme	quantitative	1208:1219	arg1	data					1221:1224	The quantitative data	1204:1224	The quantitative data for a representative set of more than hundred Na-	1204:1274	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	2	21	theme	composition	389:399	arg1	characterization					361:376	the characterization	357:376	the characterization of organic composition of heparin preparations	357:423	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	2	22	theme	compounds	502:510	arg1	quantification					474:487	the simultaneous quantification	457:487	the simultaneous quantification of inorganic compounds (water, anions, cations)	457:535	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	5	23	theme	recovery	1102:1109	arg1	precision					1051:1059	precision	1051:1059	precision	1051:1059	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	5	23	theme	recovery	1102:1109	arg1	limit					1079:1083	limit	1079:1083	limit of detection and recovery	1079:1109	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	7	24	theme	simultaneous	1482:1493	arg1	control					1508:1514	the simultaneous quantitative control	1478:1514	the simultaneous quantitative control of organic and inorganic composition of heparin	1478:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	1	25	theme	linear	151:156	arg1	glycosaminoglycan					175:191	highly sulfated glycosaminoglycan	159:191	highly sulfated glycosaminoglycan (GAG)	159:197	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	1	25	theme	linear	151:156	arg1	Heparin					138:144	Heparin	138:144	Heparin	138:144	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	1	25	theme	linear	151:156	arg1	GAG					194:196	GAG	194:196	GAG	194:196	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	0	26	theme	magnetic	8:15	arg1	resonance					17:25	Nuclear magnetic resonance	0:25	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.	0:136	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	4	27	theme	NMR	931:933	arg1	experiments					935:945	just four sequential NMR experiments	910:945	just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min	910:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	5	28	theme	detection	1088:1096	arg1	precision					1051:1059	precision	1051:1059	precision	1051:1059	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	5	28	theme	detection	1088:1096	arg1	limit					1079:1083	limit	1079:1083	limit of detection and recovery	1079:1109	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	2	29	theme	inorganic	492:500	arg1	water					513:517	water	513:517	water	513:517	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	2	29	theme	inorganic	492:500	arg1	compounds					502:510	inorganic compounds	492:510	inorganic compounds (water, anions, cations)	492:535	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	3	30	theme	inorganic	712:720	arg1	sodium					728:733	sodium	728:733	sodium	728:733	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	30	theme	inorganic	712:720	arg1	ions					722:725	the inorganic ions	708:725	the inorganic ions (sodium, calcium, and chloride)	708:757	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	30	theme	inorganic	712:720	arg1	calcium					736:742	calcium	736:742	calcium	736:742	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	30	theme	inorganic	712:720	arg1	chloride					749:756	chloride	749:756	chloride	749:756	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	0	31	theme	Nuclear	0:6	arg1	resonance					17:25	Nuclear magnetic resonance	0:25	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.	0:136	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	6	32	theme	anticoagulant	1395:1407	arg1	origin					1372:1377	animal origin	1365:1377	animal origin	1365:1377	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	32	theme	anticoagulant	1395:1407	arg1	type					1387:1390	the type	1383:1390	the type of anticoagulant	1383:1407	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	1	33	theme	β-d-glucuronic	250:263	arg1	acid					265:268	β-d-glucuronic acid	250:268	β-d-glucuronic acid	250:268	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	6	34	theme	heparin	1284:1290	arg1	samples					1332:1338	Ca- heparin and low-molecular weight heparin (LMWH) samples	1280:1338	Ca- heparin and low-molecular weight heparin (LMWH) samples	1280:1338	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	7	35	theme	organic	1519:1525	arg1	composition					1541:1551	organic and inorganic composition	1519:1551	organic and inorganic composition of heparin	1519:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	1	36	theme	sulfated	166:173	arg1	glycosaminoglycan					175:191	highly sulfated glycosaminoglycan	159:191	highly sulfated glycosaminoglycan (GAG)	159:197	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	1	36	theme	sulfated	166:173	arg1	Heparin					138:144	Heparin	138:144	Heparin	138:144	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	1	36	theme	sulfated	166:173	arg1	GAG					194:196	GAG	194:196	GAG	194:196	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	0	37	theme	resonance	17:25	arg1	spectroscopy					27:38	Nuclear magnetic resonance spectroscopy	0:38	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.	0:136	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	1	38	theme	acid	265:268	arg1	units					241:245	disaccharide units	228:245	disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine	228:309	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	3	39	theme	heparin	613:619	arg1	characterization					621:636	heparin characterization	613:636	heparin characterization	613:636	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	2	40	theme	preparations	412:423	arg1	composition					389:399	organic composition	381:399	organic composition of heparin preparations	381:423	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	6	41	theme	weight	1310:1315	arg1	LMWH					1326:1329	LMWH	1326:1329	LMWH	1326:1329	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	41	theme	weight	1310:1315	arg1	heparin					1317:1323	low-molecular weight heparin	1296:1323	low-molecular weight heparin (LMWH)	1296:1330	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	7	42	theme	suitable	1465:1472	arg1	method					1458:1463	a unique analytical method	1438:1463	a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin	1438:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	3	43	theme	magnetic	563:570	arg1	NMR					583:585	NMR	583:585	NMR	583:585	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	43	theme	magnetic	563:570	arg1	resonance					572:580	A nuclear magnetic resonance	553:580	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia	553:665	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	2	44	theme	heparin	404:410	arg1	preparations					412:423	heparin preparations	404:423	heparin preparations	404:423	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	4	45	with	possible	829:836	arg1	preparation					859:869	only one sample preparation	843:869	only one sample preparation	843:869	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	1	46	theme	α-l-iduronic	273:284	arg1	acid					286:289	α-l-iduronic acid	273:289	α-l-iduronic acid	273:289	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	2	47	dep	water	513:517	arg1	cations					528:534	cations	528:534	cations	528:534	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	2	47	dep	water	513:517	arg1	anions					520:525	anions	520:525	anions	520:525	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	7	48	theme	unique	1440:1445	arg1	method					1458:1463	a unique analytical method	1438:1463	a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin	1438:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	3	49	theme	resonance	572:580	arg1	method					602:607	A nuclear magnetic resonance (NMR) spectrometric method	553:607	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia	553:665	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	4	50	with	experiments	935:945	arg1	time					997:1000	a total measurement time	977:1000	a total measurement time less than 20 min	977:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	2	51	theme	methods	445:451	arg1	lack					437:440	a lack	435:440	a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix	435:550	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	1	52	theme	acid	286:289	arg1	units					241:245	disaccharide units	228:245	disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine	228:309	Heparin is a linear, highly sulfated glycosaminoglycan (GAG), which consists of repeating disaccharide units of β-d-glucuronic acid or α-l-iduronic acid and α-d-glucosamine.
29571131	4	53	dep	experiments	935:945	arg1	1H					948:949	1H	948:949	1H	948:949	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	6	54	theme	low-molecular	1296:1308	arg1	LMWH					1326:1329	LMWH	1326:1329	LMWH	1326:1329	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	54	theme	low-molecular	1296:1308	arg1	heparin					1317:1323	low-molecular weight heparin	1296:1323	low-molecular weight heparin (LMWH)	1296:1330	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	2	55	theme	analytical	325:334	arg1	approaches					336:345	robust analytical approaches	318:345	robust analytical approaches	318:345	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	4	56	dep	US	888:889	arg1	Pharmacopeia					891:902	Pharmacopeia	891:902	Pharmacopeia	891:902	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	7	57	theme	inorganic	1531:1539	arg1	composition					1541:1551	organic and inorganic composition	1519:1551	organic and inorganic composition of heparin	1519:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	0	58	theme	heparin	129:135	arg1	Example					118:124	Example	118:124	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.	0:136	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	3	59	theme	nuclear	555:561	arg1	NMR					583:585	NMR	583:585	NMR	583:585	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	59	theme	nuclear	555:561	arg1	resonance					572:580	A nuclear magnetic resonance	553:580	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia	553:665	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	5	60	theme	precision	1051:1059	arg1	terms					1042:1046	terms	1042:1046	terms of precision, reproducibility, limit of detection and recovery	1042:1109	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	2	61	theme	robust	318:323	arg1	approaches					336:345	robust analytical approaches	318:345	robust analytical approaches	318:345	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	4	62	theme	measurement	985:995	arg1	time					997:1000	a total measurement time	977:1000	a total measurement time less than 20 min	977:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	7	63	theme	composition	1541:1551	arg1	control					1508:1514	the simultaneous quantitative control	1478:1514	the simultaneous quantitative control of organic and inorganic composition of heparin	1478:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	4	64	theme	sample	852:857	arg1	preparation					859:869	only one sample preparation	843:869	only one sample preparation	843:869	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	0	65	from	ions	93:96	arg1	pharmaceuticals					101:115	pharmaceuticals	101:115	pharmaceuticals	101:115	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	0	66	dep	spectroscopy	27:38	arg1	Example					118:124	Example	118:124	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.	0:136	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	4	67	theme	total	979:983	arg1	time					997:1000	a total measurement time	977:1000	a total measurement time less than 20 min	977:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	3	68	theme	acetate	760:766	arg1	analysis					696:703	simultaneous analysis	683:703	simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content	683:791	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	7	69	theme	NMR	1410:1412	arg1	spectrometry					1414:1425	NMR spectrometry	1410:1425	NMR spectrometry	1410:1425	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
29571131	3	70	theme	spectrometric	588:600	arg1	method					602:607	A nuclear magnetic resonance (NMR) spectrometric method	553:607	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia	553:665	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	4	71	theme	less	1002:1005	arg1	time					997:1000	a total measurement time	977:1000	a total measurement time less than 20 min	977:1017	NMR control of these parameters is possible with only one sample preparation according to the US Pharmacopeia using just four sequential NMR experiments (1H, 2D, 23Na, and 35Cl) with a total measurement time less than 20 min.
29571131	0	72	theme	quantitative	58:69	arg1	analysis					71:78	the quantitative analysis	54:78	the quantitative analysis of water	54:87	Nuclear magnetic resonance spectroscopy as a tool for the quantitative analysis of water and ions in pharmaceuticals: Example of heparin.
29571131	6	73	theme	animal	1365:1370	arg1	origin					1372:1377	animal origin	1365:1377	animal origin	1365:1377	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	6	74	theme	heparin	1317:1323	arg1	samples					1332:1338	Ca- heparin and low-molecular weight heparin (LMWH) samples	1280:1338	Ca- heparin and low-molecular weight heparin (LMWH) samples	1280:1338	The quantitative data for a representative set of more than hundred Na- and Ca- heparin and low-molecular weight heparin (LMWH) samples were discussed regarding animal origin and the type of anticoagulant.
29571131	2	75	theme	organic	381:387	arg1	composition					389:399	organic composition	381:399	organic composition of heparin preparations	381:423	While robust analytical approaches exist for the characterization of organic composition of heparin preparations, there is a lack of methods for the simultaneous quantification of inorganic compounds (water, anions, cations) in this matrix.
29571131	5	76	from	results	1031:1037	arg1	terms					1042:1046	terms	1042:1046	terms of precision, reproducibility, limit of detection and recovery	1042:1109	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	3	77	dep	ions	722:725	arg1	sodium					728:733	sodium	728:733	sodium	728:733	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	77	dep	ions	722:725	arg1	ions					722:725	the inorganic ions	708:725	the inorganic ions (sodium, calcium, and chloride)	708:757	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	77	dep	ions	722:725	arg1	calcium					736:742	calcium	736:742	calcium	736:742	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	77	dep	ions	722:725	arg1	chloride					749:756	chloride	749:756	chloride	749:756	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	3	78	theme	simultaneous	683:694	arg1	analysis					696:703	simultaneous analysis	683:703	simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content	683:791	A nuclear magnetic resonance (NMR) spectrometric method for heparin characterization described in US Pharmacopeia was extended to simultaneous analysis of the inorganic ions (sodium, calcium, and chloride), acetate as well as water content.
29571131	5	79	theme	developed	1133:1141	arg1	method					1143:1148	the developed method	1129:1148	the developed method	1129:1148	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	5	79	theme	developed	1133:1141	arg1	fit-for-purpose					1153:1167	fit-for-purpose	1153:1167	fit-for-purpose	1153:1167	Validation results in terms of precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit-for-purpose for the authentic heparin samples.
29571131	7	80	theme	heparin	1556:1562	arg1	composition					1541:1551	organic and inorganic composition	1519:1551	organic and inorganic composition of heparin	1519:1562	NMR spectrometry represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of heparin.
31730972	2	0	mod	modified	323:330	arg1	nanofiber					309:317	cellulose nanofiber	299:317	cellulose nanofiber	299:317	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	2	0	mod	modified	323:330	arg3	groups					346:351	carboxyl groups	337:351	carboxyl groups	337:351	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	1	1	theme	cellulose	190:198	arg1	nanofiber					200:208	oxidized cellulose nanofiber	181:208	oxidized cellulose nanofiber	181:208	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	6	2	theme	red	1153:1155	arg1	cells					1163:1167	red blood cells	1153:1167	red blood cells	1153:1167	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	0	3	theme	noncompressible	92:106	arg1	hemorrhage					108:117	noncompressible hemorrhage	92:117	noncompressible hemorrhage	92:117	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	7	4	theme	good	1240:1243	arg1	biocompatibility					1245:1260	a good biocompatibility	1238:1260	a good biocompatibility for the composite aerogel	1238:1286	Hemolysis and cytotoxicity analysis results indicated a good biocompatibility for the composite aerogel.
31730972	3	5	theme	nanofiber/nanosheet	602:620	arg1	structure					633:641	a nanofiber/nanosheet interlaced structure	600:641	a nanofiber/nanosheet interlaced structure	600:641	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	6	6	theme	ability	1053:1059	arg1	measurement					1061:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	7	7	theme	composite	1270:1278	arg1	aerogel					1280:1286	the composite aerogel	1266:1286	the composite aerogel	1266:1286	Hemolysis and cytotoxicity analysis results indicated a good biocompatibility for the composite aerogel.
31730972	3	8	theme	mechanical	676:685	arg1	strength					687:694	the compressive mechanical strength	660:694	the compressive mechanical strength up to 75.4 kPa	660:709	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	6	9	theme	coagulation	1041:1051	arg1	measurement					1061:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	4	10	theme	antibacterial	841:853	arg1	ability					855:861	good antibacterial ability	836:861	good antibacterial ability	836:861	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	4	11	theme	composite	752:760	arg1	aerogel					762:768	the nanocellulose/chitosan composite aerogel	725:768	the nanocellulose/chitosan composite aerogel	725:768	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	2	12	theme	carboxyl	337:344	arg1	groups					346:351	carboxyl groups	337:351	carboxyl groups	337:351	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	4	13	theme	absorption	790:799	arg1	capacity					801:808	high water absorption capacity	779:808	high water absorption capacity	779:808	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	0	14	theme	rapid	67:71	arg1	recovery					79:86	rapid shape recovery	67:86	rapid shape recovery for noncompressible hemorrhage	67:117	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	6	15	theme	in	1032:1033	arg1	measurement					1061:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement	1028:1071	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	5	16	theme	nanocellulose5/chitosan5	939:962	arg1	aerogel					975:981	the nanocellulose5/chitosan5 compressed aerogel	935:981	the nanocellulose5/chitosan5 compressed aerogel	935:981	Once absorbing water, the nanocellulose5/chitosan5 compressed aerogel could rapidly recover its shape within 30 s.
31730972	4	17	theme	water	784:788	arg1	capacity					801:808	high water absorption capacity	779:808	high water absorption capacity	779:808	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	0	18	theme	antibacterial	11:23	arg1	nanofiber/chitosan					35:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	3	19	with	aerogel	587:593	arg1	structure					633:641	a nanofiber/nanosheet interlaced structure	600:641	a nanofiber/nanosheet interlaced structure	600:641	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	4	20	theme	high	779:782	arg1	capacity					801:808	high water absorption capacity	779:808	high water absorption capacity	779:808	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	0	21	theme	Injectable	0:9	arg1	nanofiber/chitosan					35:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	1	22	theme	rapid	227:231	arg1	hemostasis					233:242	rapid hemostasis	227:242	rapid hemostasis of noncompressible hemorrhage application	227:284	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	3	23	theme	realized	470:477	arg1	group					488:492	the realized carboxyl group	466:492	the realized carboxyl group of cellulose nanofiber	466:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	23	theme	realized	470:477	arg1	nanofiber					507:515	cellulose nanofiber	497:515	cellulose nanofiber	497:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	24	theme	compressive	664:674	arg1	strength					687:694	the compressive mechanical strength	660:694	the compressive mechanical strength up to 75.4 kPa	660:709	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	25	theme	carboxyl	479:486	arg1	group					488:492	the realized carboxyl group	466:492	the realized carboxyl group of cellulose nanofiber	466:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	25	theme	carboxyl	479:486	arg1	nanofiber					507:515	cellulose nanofiber	497:515	cellulose nanofiber	497:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	6	26	theme	good	1113:1116	arg1	adhesion					1118:1125	a good adhesion	1111:1125	a good adhesion	1111:1125	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	1	27	theme	injectable	128:137	arg1	aerogel					153:159	an injectable antibacterial aerogel	125:159	an injectable antibacterial aerogel	125:159	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	4	28	theme	shape	817:821	arg1	recovery					823:830	rapid shape recovery	811:830	rapid shape recovery	811:830	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	4	29	theme	good	836:839	arg1	ability					855:861	good antibacterial ability	836:861	good antibacterial ability	836:861	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	7	30	theme	analysis	1211:1218	arg1	results					1220:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results indicated a good biocompatibility for the composite aerogel.
31730972	3	31	theme	chitosan	550:557	arg1	amidogen					538:545	the amidogen	534:545	the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa	534:709	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	1	32	theme	noncompressible	247:261	arg1	application					274:284	noncompressible hemorrhage application	247:284	noncompressible hemorrhage application	247:284	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	0	33	theme	cellulose	25:33	arg1	nanofiber/chitosan					35:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan	0:52	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	6	34	dep	in	1032:1033	arg1	vitro					1035:1039	vitro	1035:1039	vitro	1035:1039	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	5	35	theme	compressed	964:973	arg1	aerogel					975:981	the nanocellulose5/chitosan5 compressed aerogel	935:981	the nanocellulose5/chitosan5 compressed aerogel	935:981	Once absorbing water, the nanocellulose5/chitosan5 compressed aerogel could rapidly recover its shape within 30 s.
31730972	1	36	theme	hemorrhage	263:272	arg1	application					274:284	noncompressible hemorrhage application	247:284	noncompressible hemorrhage application	247:284	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	0	37	theme	shape	73:77	arg1	recovery					79:86	rapid shape recovery	67:86	rapid shape recovery for noncompressible hemorrhage	67:117	Injectable antibacterial cellulose nanofiber/chitosan aerogel with rapid shape recovery for noncompressible hemorrhage.
31730972	1	38	theme	antibacterial	139:151	arg1	aerogel					153:159	an injectable antibacterial aerogel	125:159	an injectable antibacterial aerogel	125:159	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	3	39	theme	nanofiber	507:515	arg1	group					488:492	the realized carboxyl group	466:492	the realized carboxyl group of cellulose nanofiber	466:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	39	theme	nanofiber	507:515	arg1	nanofiber					507:515	cellulose nanofiber	497:515	cellulose nanofiber	497:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	6	40	theme	composite	1089:1097	arg1	aerogel					1099:1105	the composite aerogel	1085:1105	the composite aerogel	1085:1105	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	7	41	theme	cytotoxicity	1198:1209	arg1	results					1220:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results indicated a good biocompatibility for the composite aerogel.
31730972	2	42	theme	pressure	429:436	arg1	homogenization					438:451	high pressure homogenization	424:451	high pressure homogenization	424:451	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	6	43	contain	has	1107:1109	arg1	aerogel					1099:1105	the composite aerogel	1085:1105	the composite aerogel	1085:1105	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	6	43	contain	has	1107:1109	arg2	aggregation					1131:1141	aggregation	1131:1141	aggregation	1131:1141	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	6	43	contain	has	1107:1109	arg2	adhesion					1118:1125	a good adhesion	1111:1125	a good adhesion	1111:1125	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	4	44	theme	rapid	811:815	arg1	recovery					823:830	rapid shape recovery	811:830	rapid shape recovery	811:830	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	2	45	theme	high	424:427	arg1	homogenization					438:451	high pressure homogenization	424:451	high pressure homogenization	424:451	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	1	46	theme	application	274:284	arg1	hemostasis					233:242	rapid hemostasis	227:242	rapid hemostasis of noncompressible hemorrhage application	227:284	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	7	47	theme	Hemolysis	1184:1192	arg1	results					1220:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results	1184:1226	Hemolysis and cytotoxicity analysis results indicated a good biocompatibility for the composite aerogel.
31730972	3	48	theme	composite	577:585	arg1	aerogel					587:593	a strong composite aerogel	568:593	a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa	568:709	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	3	49	theme	strong	570:575	arg1	aerogel					587:593	a strong composite aerogel	568:593	a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa	568:709	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	6	50	dep	adhesion	1118:1125	arg1	effect					1143:1148	effect	1143:1148	a good adhesion and aggregation effect	1111:1148	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
31730972	3	51	theme	interlaced	622:631	arg1	structure					633:641	a nanofiber/nanosheet interlaced structure	600:641	a nanofiber/nanosheet interlaced structure	600:641	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	4	52	theme	nanocellulose/chitosan	729:750	arg1	aerogel					762:768	the nanocellulose/chitosan composite aerogel	725:768	the nanocellulose/chitosan composite aerogel	725:768	In addition, the nanocellulose/chitosan composite aerogel exhibits high water absorption capacity, rapid shape recovery and good antibacterial ability (via Escherichia coli and Staphylococcus aureus).
31730972	3	53	theme	cellulose	497:505	arg1	nanofiber					507:515	cellulose nanofiber	497:515	cellulose nanofiber	497:515	Whereafter, the realized carboxyl group of cellulose nanofiber was reacted with the amidogen of chitosan to yield a strong composite aerogel with a nanofiber/nanosheet interlaced structure, which increased the compressive mechanical strength up to 75.4 kPa.
31730972	2	54	theme	cellulose	299:307	arg1	nanofiber					309:317	cellulose nanofiber	299:317	cellulose nanofiber	299:317	Especially, cellulose nanofiber was modified with carboxyl groups by pre-oxidizing in 2,2,6,6-tetramethylpiperidine-1-oxyl combined with high pressure homogenization.
31730972	1	55	theme	oxidized	181:188	arg1	nanofiber					200:208	oxidized cellulose nanofiber	181:208	oxidized cellulose nanofiber	181:208	Here an injectable antibacterial aerogel was fabricated with oxidized cellulose nanofiber and chitosan for rapid hemostasis of noncompressible hemorrhage application.
31730972	6	56	theme	blood	1157:1161	arg1	cells					1163:1167	red blood cells	1153:1167	red blood cells	1153:1167	The in vitro coagulation ability measurement showed that the composite aerogel has a good adhesion and aggregation effect to red blood cells and platelets.
30791055	3	0	from	incidents	694:702	arg1	Brazil					707:712	Brazil	707:712	Brazil	707:712	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	4	1	theme	protamine	1134:1142	arg1	neutralization					1144:1157	protamine neutralization	1134:1157	protamine neutralization	1134:1157	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	3	2	theme	Brazilian	807:815	arg1	Pharmacopoeia					817:829	the Brazilian Pharmacopoeia	803:829	the Brazilian Pharmacopoeia	803:829	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	3	3	theme	first	757:761	arg1	monographs					763:772	the first monographs	753:772	the first monographs exclusive for HBI and HPI	753:798	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	4	4	theme	HBI	929:931	arg1	derivative					933:942	a novel HBI derivative	921:942	a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI	921:1205	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	5	theme	heparin-induced	1085:1099	arg1	potentials					1119:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	1	6	theme	low-molecular-weight	144:163	arg1	derivatives					165:175	their low-molecular-weight derivatives	138:175	their low-molecular-weight derivatives	138:175	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	3	7	theme	monographs	763:772	arg1	publication					738:748	the publication	734:748	the publication of the first monographs exclusive for HBI and HPI	734:798	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	0	8	theme	Anticoagulant	85:97	arg1	Drug					99:102	a Single Anticoagulant Drug	76:102	a Single Anticoagulant Drug	76:102	Converting the Distinct Heparins Sourced from Bovine or Porcine Mucosa into a Single Anticoagulant Drug.
30791055	2	9	contain	has	405:407	arg2	half					450:453	about half	444:453	about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively)	444:546	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	9	contain	has	405:407	arg1	HBI					401:403	HBI	401:403	HBI	401:403	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	9	contain	has	405:407	arg2	composition					428:438	different chemical composition	409:438	different chemical composition	409:438	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	0	10	theme	Single	78:83	arg1	Drug					99:102	a Single Anticoagulant Drug	76:102	a Single Anticoagulant Drug	76:102	Converting the Distinct Heparins Sourced from Bovine or Porcine Mucosa into a Single Anticoagulant Drug.
30791055	1	11	from	mucosa	288:293	arg1	introduction					256:267	a worldwide introduction	244:267	a worldwide introduction of UFH from bovine mucosa (HBI)	244:299	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	4	12	dep	composition	997:1007	arg1	enriched					1010:1017	enriched	1010:1017	enriched in N,6-disulfated α-glucosamine	1010:1049	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	5	13	theme	pharmacological	1279:1293	arg1	characteristics					1295:1309	pharmacological characteristics	1279:1309	pharmacological characteristics	1279:1309	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	1	14	theme	worldwide	246:254	arg1	introduction					256:267	a worldwide introduction	244:267	a worldwide introduction of UFH from bovine mucosa (HBI)	244:299	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	2	15	theme	HPI	487:489	arg1	potency					476:482	the anticoagulant potency	458:482	the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively)	458:546	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	16	from	UFHs	592:595	arg1	countries					605:613	some countries	600:613	some countries since the 1990s	600:629	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	17	theme	interchangeable	576:590	arg1	they					549:552	they	549:552	they	549:552	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	17	theme	interchangeable	576:590	arg1	UFHs					592:595	interchangeable UFHs	576:595	interchangeable UFHs in some countries since the 1990s	576:629	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	4	18	theme	bleeding	1072:1079	arg1	potentials					1119:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	19	theme	anion-exchange	891:904	arg1	chromatography					906:919	high-resolution anion-exchange chromatography	875:919	high-resolution anion-exchange chromatography	875:919	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	20	theme	thrombocytopaenia	1101:1117	arg1	potentials					1119:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	2	21	theme	chemical	419:426	arg1	composition					428:438	different chemical composition	409:438	different chemical composition	409:438	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	4	22	theme	novel	923:927	arg1	derivative					933:942	a novel HBI derivative	921:942	a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI	921:1205	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	23	theme	high-resolution	875:889	arg1	chromatography					906:919	high-resolution anion-exchange chromatography	875:919	high-resolution anion-exchange chromatography	875:919	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	3	24	theme	single	656:661	arg1	drug					663:666	a single drug	654:666	a single drug	654:666	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	2	25	theme	different	409:417	arg1	composition					428:438	different chemical composition	409:438	different chemical composition	409:438	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	3	26	theme	several	677:683	arg1	incidents					694:702	several bleeding incidents	677:702	several bleeding incidents	677:702	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	5	27	theme	HBI	1390:1392	arg1	manufacturing					1394:1406	the HBI manufacturing	1386:1406	the HBI manufacturing	1386:1406	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	4	28	theme	safety	1056:1061	arg1	profile					1063:1069	safety profile	1056:1069	safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization)	1056:1158	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	3	29	theme	bleeding	685:692	arg1	incidents					694:702	several bleeding incidents	677:702	several bleeding incidents	677:702	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	2	30	theme	unit	520:523	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	0	31	theme	Distinct	15:22	arg1	Heparins					24:31	the Distinct Heparins	11:31	the Distinct Heparins Sourced from Bovine or Porcine Mucosa	11:69	Converting the Distinct Heparins Sourced from Bovine or Porcine Mucosa into a Single Anticoagulant Drug.
30791055	1	32	theme	UFH	272:274	arg1	introduction					256:267	a worldwide introduction	244:267	a worldwide introduction of UFH from bovine mucosa (HBI)	244:299	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	2	33	theme	international	506:518	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	1	34	theme	unsteady	349:356	arg1	chain					365:369	the currently unsteady supply chain	335:369	the currently unsteady supply chain of heparin products	335:389	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	5	35	from	improvement	1371:1381	arg1	manufacturing					1394:1406	the HBI manufacturing	1386:1406	the HBI manufacturing	1386:1406	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	4	36	theme	anticoagulant	949:961	arg1	potency					963:969	anticoagulant potency	949:969	anticoagulant potency (200 IU/mg)	949:981	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	36	theme	anticoagulant	949:961	arg1	IU/mg					976:980	200 IU/mg	972:980	200 IU/mg	972:980	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	2	37	theme	∼180	501:504	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	1	38	theme	supply	358:363	arg1	chain					365:369	the currently unsteady supply chain	335:369	the currently unsteady supply chain of heparin products	335:389	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	1	39	dep	sourced	181:187	arg1	HPI					229:231	HPI	229:231	HPI	229:231	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	1	40	theme	porcine	213:219	arg1	mucosa					221:226	porcine mucosa	213:226	porcine mucosa	213:226	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	1	41	theme	bovine	281:286	arg1	mucosa					288:293	bovine mucosa	281:293	bovine mucosa (HBI)	281:299	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	1	41	theme	bovine	281:286	arg1	HBI					296:298	HBI	296:298	HBI	296:298	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	0	42	dep	Bovine	46:51	arg1	Mucosa					64:69	Mucosa	64:69	Mucosa	64:69	Converting the Distinct Heparins Sourced from Bovine or Porcine Mucosa into a Single Anticoagulant Drug.
30791055	4	43	theme	similar	1160:1166	arg1	derivative					933:942	a novel HBI derivative	921:942	a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI	921:1205	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	2	44	theme	∼100	492:495	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	4	45	dep	profile	1063:1069	arg1	potentials					1119:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	bleeding and heparin-induced thrombocytopaenia potentials	1072:1128	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	45	dep	profile	1063:1069	arg1	neutralization					1144:1157	protamine neutralization	1134:1157	protamine neutralization	1134:1157	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	1	46	theme	Unfractionated	105:118	arg1	UFH					129:131	UFH	129:131	UFH	129:131	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	1	46	theme	Unfractionated	105:118	arg1	heparin					120:126	Unfractionated heparin	105:126	Unfractionated heparin (UFH)	105:132	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	5	47	dep	composition	1263:1273	arg1	the					1259:1261	the	1259:1261	the	1259:1261	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	2	48	dep	HPI	487:489	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	1	49	theme	heparin	374:380	arg1	products					382:389	heparin products	374:389	heparin products	374:389	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	5	50	theme	implementable	1357:1369	arg1	improvement					1371:1381	an easily implementable improvement	1347:1381	an easily implementable improvement in the HBI manufacturing	1347:1406	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	4	51	theme	N,6-disulfated	1022:1035	arg1	α-glucosamine					1037:1049	N,6-disulfated α-glucosamine	1022:1049	N,6-disulfated α-glucosamine	1022:1049	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	1	52	theme	products	382:389	arg1	chain					365:369	the currently unsteady supply chain	335:369	the currently unsteady supply chain of heparin products	335:389	Unfractionated heparin (UFH) and their low-molecular-weight derivatives are sourced almost exclusively from porcine mucosa (HPI); however, a worldwide introduction of UFH from bovine mucosa (HBI) has been recommended to reinforce the currently unsteady supply chain of heparin products.
30791055	4	53	theme	standard	1194:1201	arg1	HPI					1203:1205	the gold standard HPI	1185:1205	the gold standard HPI	1185:1205	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	2	54	theme	[IU	525:527	arg1	/mg					529:531	∼100 and ∼180 international unit [IU]/mg	492:531	/mg	529:531	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	4	55	theme	disaccharide	984:995	arg1	composition					997:1007	disaccharide composition	984:1007	disaccharide composition (enriched in N,6-disulfated α-glucosamine)	984:1050	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	56	with	derivative	933:942	arg1	potency					963:969	anticoagulant potency	949:969	anticoagulant potency (200 IU/mg)	949:981	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	56	with	derivative	933:942	arg1	profile					1063:1069	safety profile	1056:1069	safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization)	1056:1158	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	56	with	derivative	933:942	arg1	composition					997:1007	disaccharide composition	984:1007	disaccharide composition (enriched in N,6-disulfated α-glucosamine)	984:1050	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	4	56	with	derivative	933:942	arg1	IU/mg					976:980	200 IU/mg	972:980	200 IU/mg	972:980	Nevertheless, we succeed in producing with high-resolution anion-exchange chromatography a novel HBI derivative with anticoagulant potency (200 IU/mg), disaccharide composition (enriched in N,6-disulfated α-glucosamine) and safety profile (bleeding and heparin-induced thrombocytopaenia potentials and protamine neutralization) similar to those seen in the gold standard HPI.
30791055	5	57	theme	distinct	1320:1327	arg1	UFHs					1329:1332	these distinct UFHs	1314:1332	these distinct UFHs	1314:1332	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	3	58	theme	exclusive	774:782	arg1	monographs					763:772	the first monographs	753:772	the first monographs exclusive for HBI and HPI	753:798	However, their use as a single drug provoked several bleeding incidents in Brazil, which precipitated the publication of the first monographs exclusive for HBI and HPI by the Brazilian Pharmacopoeia.
30791055	2	59	theme	potency	476:482	arg1	composition					428:438	different chemical composition	409:438	different chemical composition	409:438	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	2	59	theme	potency	476:482	arg1	half					450:453	about half	444:453	about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively)	444:546	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
30791055	5	60	theme	UFHs	1329:1332	arg1	composition					1263:1273	composition	1263:1273	composition	1263:1273	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	5	60	theme	UFHs	1329:1332	arg1	characteristics					1295:1309	pharmacological characteristics	1279:1309	pharmacological characteristics	1279:1309	Therefore, we show that it is possible to equalize the composition and pharmacological characteristics of these distinct UFHs by employing an easily implementable improvement in the HBI manufacturing.
30791055	2	61	theme	anticoagulant	462:474	arg1	potency					476:482	the anticoagulant potency	458:482	the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively)	458:546	Although HBI has different chemical composition and about half of the anticoagulant potency of HPI (∼100 and ∼180 international unit [IU]/mg, respectively), they have been employed as interchangeable UFHs in some countries since the 1990s.
31403146	5	0	theme	μg	705:706	arg1	kg-1					708:711	804 μg kg-1	701:711	804 μg kg-1	701:711	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	8	1	theme	improved	1028:1035	arg1	quality					1045:1051	improved sensory quality	1028:1051	improved sensory quality along with a favourable healthy composition of barley products	1028:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	6	2	theme	protein	784:790	arg1	contents					767:774	the contents	763:774	the contents of fat, protein, starch and β-glucan	763:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	1	3	theme	barley	170:175	arg1	consumption					141:151	The consumption	137:151	The consumption of β-glucan-rich barley	137:175	The consumption of β-glucan-rich barley can contribute to a healthy diet.
31403146	3	4	theme	present	324:330	arg1	study					332:336	the present study	320:336	the present study	320:336	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	8	5	dep	along	1053:1057	arg1	with					1059:1062	with	1059:1062	with	1059:1062	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	5	6	theme	Acrylamide	583:592	arg1	levels					594:599	Acrylamide levels	583:599	Acrylamide levels	583:599	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	3	7	theme	barley	502:507	arg1	kernels					509:515	β-glucan-rich barley kernels	488:515	β-glucan-rich barley kernels	488:515	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	0	8	from	Impact	0:5	arg1	quality					73:79	sensory quality	65:79	sensory quality	65:79	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	0	8	from	Impact	0:5	arg1	properties					101:110	physicochemical properties	85:110	physicochemical properties	85:110	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	0	8	from	Impact	0:5	arg1	composition					52:62	chemical composition	43:62	chemical composition	43:62	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	6	9	theme	relevant	732:739	arg1	impact					741:746	No relevant impact	729:746	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan	729:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	3	10	theme	thick	527:531	arg1	quality					437:443	sensory quality	429:443	sensory quality	429:443	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	10	theme	thick	527:531	arg1	flakes					533:538	thin and thick flakes	518:538	thin and thick flakes	518:538	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	0	11	theme	physicochemical	85:99	arg1	properties					101:110	physicochemical properties	85:110	physicochemical properties	85:110	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	5	12	theme	μg	652:653	arg1	kernels					661:667	kernels	661:667	kernels	661:667	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	5	12	theme	μg	652:653	arg1	flakes					720:725	thick flakes	714:725	thick flakes	714:725	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	5	12	theme	μg	652:653	arg1	kg-1					655:658	322 μg kg-1	648:658	322 μg kg-1 (kernels)	648:668	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	3	13	theme	β-glucan-rich	488:500	arg1	kernels					509:515	β-glucan-rich barley kernels	488:515	β-glucan-rich barley kernels	488:515	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	7	14	dep	1.9-fold	929:936	arg1	flakes					992:997	thick flakes	986:997	thick flakes	986:997	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	7	14	dep	1.9-fold	929:936	arg1	kernels					939:945	kernels	939:945	kernels	939:945	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	7	14	dep	1.9-fold	929:936	arg1	2.4-fold					949:956	2.4-fold	949:956	2.4-fold	949:956	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	7	14	dep	1.9-fold	929:936	arg1	flakes					964:969	thin flakes	959:969	thin flakes	959:969	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	6	15	dep	observed	817:824	arg1	whereas					827:833	whereas	827:833	whereas	827:833	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	0	16	theme	waxy-barley	115:125	arg1	products					127:134	waxy-barley products	115:134	waxy-barley products	115:134	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	6	17	theme	starch	793:798	arg1	contents					767:774	the contents	763:774	the contents of fat, protein, starch and β-glucan	763:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	3	18	theme	thin	518:521	arg1	quality					437:443	sensory quality	429:443	sensory quality	429:443	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	18	theme	thin	518:521	arg1	flakes					533:538	thin and thick flakes	518:538	thin and thick flakes	518:538	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	8	19	theme	healthy	1077:1083	arg1	composition					1085:1095	a favourable healthy composition	1064:1095	improved sensory quality along with a favourable healthy composition of barley products	1028:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	5	20	theme	thick	714:718	arg1	flakes					720:725	thick flakes	714:725	thick flakes	714:725	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	5	20	theme	thick	714:718	arg1	kg-1					655:658	322 μg kg-1	648:658	322 μg kg-1 (kernels)	648:668	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	8	21	theme	products	1107:1114	arg1	composition					1085:1095	a favourable healthy composition	1064:1095	improved sensory quality along with a favourable healthy composition of barley products	1028:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	3	22	from	compounds	461:469	arg1	kernels					509:515	β-glucan-rich barley kernels	488:515	β-glucan-rich barley kernels	488:515	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	23	theme	roasting	381:388	arg1	conditions					390:399	different roasting conditions	371:399	different roasting conditions (160-200 °C, 20 minutes)	371:424	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	23	theme	roasting	381:388	arg1	°C					410:411	160-200 °C	402:411	160-200 °C	402:411	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	5	24	theme	kg-1	708:711	arg1	values					638:643	maximum values	630:643	maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes)	630:726	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	3	25	dep	°C	410:411	arg1	minutes					417:423	20 minutes	414:423	20 minutes	414:423	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	26	theme	study	332:336	arg1	aim					313:315	The aim	309:315	The aim of the present study	309:336	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	6	27	from	impact	741:746	arg1	contents					767:774	the contents	763:774	the contents of fat, protein, starch and β-glucan	763:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	3	28	theme	conditions	390:399	arg1	impact					361:366	the impact	357:366	the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes	357:538	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	4	29	theme	Sensory	541:547	arg1	quality					549:555	Sensory quality	541:555	Sensory quality	541:555	Sensory quality was improved by roasting.
31403146	5	30	theme	maximum	630:636	arg1	values					638:643	maximum values	630:643	maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes)	630:726	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	3	31	from	viscosity	475:483	arg1	kernels					509:515	β-glucan-rich barley kernels	488:515	β-glucan-rich barley kernels	488:515	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	1	32	theme	healthy	197:203	arg1	diet					205:208	a healthy diet	195:208	a healthy diet	195:208	The consumption of β-glucan-rich barley can contribute to a healthy diet.
31403146	0	33	theme	roasting	20:27	arg1	conditions					29:38	different roasting conditions	10:38	different roasting conditions	10:38	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	3	34	theme	different	371:379	arg1	conditions					390:399	different roasting conditions	371:399	different roasting conditions (160-200 °C, 20 minutes)	371:424	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	34	theme	different	371:379	arg1	°C					410:411	160-200 °C	402:411	160-200 °C	402:411	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	8	35	theme	sensory	1037:1043	arg1	quality					1045:1051	improved sensory quality	1028:1051	improved sensory quality along with a favourable healthy composition of barley products	1028:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	0	36	theme	products	127:134	arg1	quality					73:79	sensory quality	65:79	sensory quality	65:79	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	0	36	theme	products	127:134	arg1	properties					101:110	physicochemical properties	85:110	physicochemical properties	85:110	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	0	36	theme	products	127:134	arg1	composition					52:62	chemical composition	43:62	chemical composition	43:62	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	3	37	theme	health-related	446:459	arg1	compounds					461:469	health-related compounds	446:469	health-related compounds	446:469	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	8	38	theme	barley	1100:1105	arg1	products					1107:1114	barley products	1100:1114	barley products	1100:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	5	39	theme	μg	675:676	arg1	flakes					689:694	thin flakes	684:694	thin flakes	684:694	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	5	39	theme	μg	675:676	arg1	kg-1					678:681	586 μg kg-1	671:681	586 μg kg-1 (thin flakes)	671:695	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	6	40	theme	fat	779:781	arg1	contents					767:774	the contents	763:774	the contents of fat, protein, starch and β-glucan	763:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	3	41	theme	sensory	429:435	arg1	quality					437:443	sensory quality	429:443	sensory quality	429:443	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	41	theme	sensory	429:435	arg1	flakes					533:538	thin and thick flakes	518:538	thin and thick flakes	518:538	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	0	42	theme	conditions	29:38	arg1	Impact					0:5	Impact	0:5	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.	0:135	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	5	43	theme	kg-1	678:681	arg1	values					638:643	maximum values	630:643	maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes)	630:726	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	8	44	theme	low	1151:1153	arg1	range					1177:1181	a low to medium temperature range	1149:1181	a low to medium temperature range	1149:1181	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	6	45	theme	fibre	843:847	arg1	fractions					849:857	dietary fibre fractions	835:857	dietary fibre fractions	835:857	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	5	46	theme	kg-1	655:658	arg1	values					638:643	maximum values	630:643	maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes)	630:726	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	3	47	from	impact	361:366	arg1	viscosity					475:483	viscosity	475:483	viscosity	475:483	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	47	from	impact	361:366	arg1	quality					437:443	sensory quality	429:443	sensory quality	429:443	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	47	from	impact	361:366	arg1	compounds					461:469	health-related compounds	446:469	health-related compounds	446:469	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	47	from	impact	361:366	arg1	flakes					533:538	thin and thick flakes	518:538	thin and thick flakes	518:538	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	3	48	from	quality	437:443	arg1	kernels					509:515	β-glucan-rich barley kernels	488:515	β-glucan-rich barley kernels	488:515	The aim of the present study was to investigate the impact of different roasting conditions (160-200 °C, 20 minutes) on sensory quality, health-related compounds and viscosity in β-glucan-rich barley kernels, thin and thick flakes.
31403146	8	49	theme	temperature	1165:1175	arg1	range					1177:1181	a low to medium temperature range	1149:1181	a low to medium temperature range	1149:1181	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	0	50	theme	chemical	43:50	arg1	composition					52:62	chemical composition	43:62	chemical composition	43:62	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	6	51	theme	dietary	835:841	arg1	fractions					849:857	dietary fibre fractions	835:857	dietary fibre fractions	835:857	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	7	52	theme	thin	959:962	arg1	flakes					964:969	thin flakes	959:969	thin flakes	959:969	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	8	53	theme	favourable	1066:1075	arg1	composition					1085:1095	a favourable healthy composition	1064:1095	improved sensory quality along with a favourable healthy composition of barley products	1028:1114	In conclusion, improved sensory quality along with a favourable healthy composition of barley products may be achieved by roasting over a low to medium temperature range.
31403146	2	54	theme	Sensory	211:217	arg1	properties					219:228	Sensory properties	211:228	Sensory properties	211:228	Sensory properties may be improved by roasting whereby the nutritional value should be preserved.
31403146	2	55	theme	nutritional	270:280	arg1	value					282:286	the nutritional value	266:286	the nutritional value	266:286	Sensory properties may be improved by roasting whereby the nutritional value should be preserved.
31403146	6	56	theme	roasting	751:758	arg1	impact					741:746	No relevant impact	729:746	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan	729:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	7	57	theme	thick	986:990	arg1	flakes					992:997	thick flakes	986:997	thick flakes	986:997	Roasting significantly decreased viscosity 1.9-fold (kernels), 2.4-fold (thin flakes) and 2.7-fold (thick flakes), on average.
31403146	0	58	theme	sensory	65:71	arg1	quality					73:79	sensory quality	65:79	sensory quality	65:79	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	6	59	theme	β-glucan	804:811	arg1	contents					767:774	the contents	763:774	the contents of fat, protein, starch and β-glucan	763:811	No relevant impact of roasting on the contents of fat, protein, starch and β-glucan was observed, whereas dietary fibre fractions were marginally modulated.
31403146	5	60	theme	804	701:703	arg1	μg					705:706	μg	705:706	μg	705:706	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	0	61	theme	different	10:18	arg1	conditions					29:38	different roasting conditions	10:38	different roasting conditions	10:38	Impact of different roasting conditions on chemical composition, sensory quality and physicochemical properties of waxy-barley products.
31403146	1	62	theme	β-glucan-rich	156:168	arg1	barley					170:175	β-glucan-rich barley	156:175	β-glucan-rich barley	156:175	The consumption of β-glucan-rich barley can contribute to a healthy diet.
31403146	5	63	theme	thin	684:687	arg1	flakes					689:694	thin flakes	684:694	thin flakes	684:694	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
31403146	5	63	theme	thin	684:687	arg1	kg-1					678:681	586 μg kg-1	671:681	586 μg kg-1 (thin flakes)	671:695	Acrylamide levels increased due to roasting to maximum values of 322 μg kg-1 (kernels), 586 μg kg-1 (thin flakes) and 804 μg kg-1 (thick flakes).
30870530	11	0	theme	supporting	2188:2197	arg1	division					2199:2206	supporting division	2188:2206	supporting division	2188:2206	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	9	1	theme	cycle	1810:1814	arg1	inhibition					1816:1825	TCA cycle inhibition	1806:1825	TCA cycle inhibition	1806:1825	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	3	2	theme	S.	720:721	arg1	protein-					735:742	S. epidermidis protein- and PIA-biofilm matrix producers	720:775	protein-	735:742	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	3	theme	RNA	589:591	arg1	dRNA-seq					605:612	dRNA-seq	605:612	dRNA-seq	605:612	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	3	theme	RNA	589:591	arg1	sequencing					593:602	differential RNA sequencing	576:602	differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM)	576:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	1	4	theme	metabolic	265:273	arg1	pathways					275:282	other metabolic pathways	259:282	other metabolic pathways	259:282	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	9	5	theme	icaADBC	1743:1749	arg1	expression					1758:1767	icaADBC operon expression	1743:1767	icaADBC operon expression	1743:1767	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	3	6	theme	PIA-biofilm	748:758	arg1	producers					767:775	S. epidermidis protein- and PIA-biofilm matrix producers	720:775	producers	767:775	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	9	7	theme	expression	1758:1767	arg1	activation					1729:1738	activation	1729:1738	activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis	1729:1920	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	10	8	theme	eDNA	1997:2000	arg1	release					1986:1992	the release	1982:1992	the release of eDNA	1982:2000	Additionally, RsaE supports biofilm formation by mediating the release of eDNA as stabilizing biofilm matrix component.
30870530	11	9	dep	S.	2220:2221	arg1	epidermidis					2223:2233	epidermidis	2223:2233	epidermidis	2223:2233	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	4	10	dep	S.	1002:1003	arg1	epidermidis					1005:1015	epidermidis	1005:1015	epidermidis	1005:1015	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	9	11	theme	icaR	1787:1790	arg1	repression					1773:1782	repression	1773:1782	repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis	1773:1920	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	8	12	theme	sucCD	1510:1514	arg1	mRNAs					1516:1520	icaR and sucCD mRNAs	1501:1520	mRNAs	1516:1520	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	6	13	theme	spectrum	1307:1314	arg1	expansion					1278:1286	expansion	1278:1286	expansion of the mRNA target spectrum	1278:1314	RsaE processing results in expansion of the mRNA target spectrum.
30870530	3	14	theme	gene	819:822	arg1	expression					824:833	metabolic gene expression	809:833	metabolic gene expression	809:833	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	5	15	theme	full-length	1139:1149	arg1	transcript					1158:1167	a major full-length 100-nt transcript	1131:1167	a major full-length 100-nt transcript	1131:1167	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	3	16	theme	confocal	635:642	arg1	CLSM					671:674	CLSM	671:674	CLSM	671:674	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	16	theme	confocal	635:642	arg1	microscopy					659:668	confocal laser scanning microscopy	635:668	confocal laser scanning microscopy (CLSM)	635:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	4	17	theme	biofilm	954:960	arg1	phenotype					962:970	a PIA biofilm phenotype	948:970	a PIA biofilm phenotype as well as eDNA release	948:994	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	0	18	from	composition	62:72	arg1	communities					112:122	Staphylococcus epidermidis biofilm communities	77:122	Staphylococcus epidermidis biofilm communities	77:122	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	6	19	theme	mRNA	1295:1298	arg1	spectrum					1307:1314	the mRNA target spectrum	1291:1314	the mRNA target spectrum	1291:1314	RsaE processing results in expansion of the mRNA target spectrum.
30870530	11	20	from	heterogeneity	2170:2182	arg1	communities					2243:2253	S. epidermidis biofilm communities	2220:2253	S. epidermidis biofilm communities	2220:2253	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	1	21	theme	small	145:149	arg1	RNA					162:164	small regulatory RNA	145:164	a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis	133:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	21	theme	small	145:149	arg1	RsaE					125:128	RsaE	125:128	RsaE	125:128	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	21	theme	small	145:149	arg1	sRNA					167:170	sRNA	167:170	sRNA	167:170	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	9	22	theme	metabolism	1879:1888	arg1	re-programming					1831:1844	re-programming	1831:1844	re-programming	1831:1844	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	9	22	theme	metabolism	1879:1888	arg1	inhibition					1816:1825	TCA cycle inhibition	1806:1825	TCA cycle inhibition	1806:1825	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	4	23	theme	biofilm	1025:1031	arg1	background					1057:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	8	24	theme	biofilm	1544:1550	arg1	repressor					1559:1567	the icaADBC biofilm operon repressor	1532:1567	the icaADBC biofilm operon repressor IcaR	1532:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	8	24	theme	biofilm	1544:1550	arg1	IcaR					1569:1572	IcaR	1569:1572	IcaR	1569:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	5	25	theme	processed	1181:1189	arg1	species					1191:1197	a minor processed species	1173:1197	a minor processed species lacking approximately 20 nucleotides at the 5'-end	1173:1248	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	3	26	theme	scanning	650:657	arg1	CLSM					671:674	CLSM	671:674	CLSM	671:674	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	26	theme	scanning	650:657	arg1	microscopy					659:668	confocal laser scanning microscopy	635:668	confocal laser scanning microscopy (CLSM)	635:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	4	27	theme	strain	1050:1055	arg1	background					1057:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	0	28	theme	Staphylococcus	77:90	arg1	communities					112:122	Staphylococcus epidermidis biofilm communities	77:122	Staphylococcus epidermidis biofilm communities	77:122	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	0	29	theme	biofilm	104:110	arg1	communities					112:122	Staphylococcus epidermidis biofilm communities	77:122	Staphylococcus epidermidis biofilm communities	77:122	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	9	30	theme	central	1864:1870	arg1	metabolism					1879:1888	staphylococcal central carbon metabolism	1849:1888	staphylococcal central carbon metabolism	1849:1888	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	2	31	theme	DNA	387:389	arg1	release					391:397	extracellular (e)DNA release	370:397	extracellular (e)DNA release	370:397	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	2	32	theme	adhesin	465:471	arg1	production					479:488	polysaccharide intercellular adhesin (PIA) production	436:488	polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate	436:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	2	33	theme	Staphylococcus	509:522	arg1	isolate					536:542	a hypervariable Staphylococcus epidermidis isolate	493:542	a hypervariable Staphylococcus epidermidis isolate	493:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	2	34	theme	e	385:385	arg1	release					391:397	extracellular (e)DNA release	370:397	extracellular (e)DNA release	370:397	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	1	35	theme	pathways	275:282	arg1	riboregulator					218:230	a riboregulator	216:230	a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis	216:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	0	36	dep	Staphylococcus	77:90	arg1	epidermidis					92:102	epidermidis	92:102	epidermidis	92:102	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	10	37	theme	biofilm	1951:1957	arg1	formation					1959:1967	biofilm formation	1951:1967	biofilm formation	1951:1967	Additionally, RsaE supports biofilm formation by mediating the release of eDNA as stabilizing biofilm matrix component.
30870530	2	38	theme	polysaccharide	436:449	arg1	production					479:488	polysaccharide intercellular adhesin (PIA) production	436:488	polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate	436:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	8	39	theme	acid	1623:1626	arg1	cycle					1634:1638	the tricarboxylic acid (TCA) cycle	1605:1638	the tricarboxylic acid (TCA) cycle	1605:1638	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	9	40	theme	PIA	1898:1900	arg1	synthesis					1912:1920	PIA precursor synthesis	1898:1920	PIA precursor synthesis	1898:1920	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	0	41	theme	non-coding	10:19	arg1	RsaE					25:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	8	42	theme	TCA	1629:1631	arg1	cycle					1634:1638	the tricarboxylic acid (TCA) cycle	1605:1638	the tricarboxylic acid (TCA) cycle	1605:1638	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	3	43	theme	reporter	681:688	arg1	fusions					695:701	reporter gene fusions	681:701	reporter gene fusions	681:701	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	5	44	theme	blot	1091:1094	arg1	analyses					1096:1103	dRNA-seq and Northern blot analyses	1069:1103	analyses	1096:1103	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	2	45	theme	PIA	474:476	arg1	production					479:488	polysaccharide intercellular adhesin (PIA) production	436:488	polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate	436:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	8	46	theme	cycle	1634:1638	arg1	repressor					1559:1567	the icaADBC biofilm operon repressor	1532:1567	the icaADBC biofilm operon repressor IcaR	1532:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	8	46	theme	cycle	1634:1638	arg1	IcaR					1569:1572	IcaR	1569:1572	IcaR	1569:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	8	46	theme	cycle	1634:1638	arg1	synthetase					1591:1600	succinyl-CoA synthetase	1578:1600	succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle	1578:1638	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	4	47	theme	protein	1017:1023	arg1	background					1057:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	4	48	theme	epidermidis	881:891	arg1	biofilms					906:913	S. epidermidis PIA-mediated biofilms	878:913	S. epidermidis PIA-mediated biofilms	878:913	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	10	49	theme	matrix	2025:2030	arg1	component					2032:2040	stabilizing biofilm matrix component	2005:2040	stabilizing biofilm matrix component	2005:2040	Additionally, RsaE supports biofilm formation by mediating the release of eDNA as stabilizing biofilm matrix component.
30870530	0	50	theme	extracellular	41:53	arg1	composition					62:72	extracellular matrix composition	41:72	extracellular matrix composition in Staphylococcus epidermidis biofilm communities	41:122	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	9	51	theme	operon	1751:1756	arg1	expression					1758:1767	icaADBC operon expression	1743:1767	icaADBC operon expression	1743:1767	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	2	52	theme	extracellular	370:382	arg1	release					391:397	extracellular (e)DNA release	370:397	extracellular (e)DNA release	370:397	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	1	53	theme	central	235:241	arg1	flow					250:253	central carbon flow	235:253	central carbon flow	235:253	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	11	54	from	division	2199:2206	arg1	communities					2243:2253	S. epidermidis biofilm communities	2220:2253	S. epidermidis biofilm communities	2220:2253	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	7	55	theme	full-length	1323:1333	arg1	RsaE					1335:1338	full-length RsaE	1323:1338	full-length RsaE	1323:1338	Thus, full-length RsaE interacts with S. epidermidis antiholin-encoding lrgA mRNA, facilitating bacterial lysis and eDNA release.
30870530	10	56	theme	biofilm	2017:2023	arg1	component					2032:2040	stabilizing biofilm matrix component	2005:2040	stabilizing biofilm matrix component	2005:2040	Additionally, RsaE supports biofilm formation by mediating the release of eDNA as stabilizing biofilm matrix component.
30870530	1	57	theme	flow	250:253	arg1	riboregulator					218:230	a riboregulator	216:230	a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis	216:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	11	58	theme	S.	2220:2221	arg1	communities					2243:2253	S. epidermidis biofilm communities	2220:2253	S. epidermidis biofilm communities	2220:2253	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	3	59	theme	epidermidis	723:733	arg1	protein-					735:742	S. epidermidis protein- and PIA-biofilm matrix producers	720:775	protein-	735:742	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	60	theme	differential	576:587	arg1	dRNA-seq					605:612	dRNA-seq	605:612	dRNA-seq	605:612	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	60	theme	differential	576:587	arg1	sequencing					593:602	differential RNA sequencing	576:602	differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM)	576:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	1	61	theme	other	259:263	arg1	pathways					275:282	other metabolic pathways	259:282	other metabolic pathways	259:282	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	11	62	theme	labor	2211:2215	arg1	heterogeneity					2170:2182	phenotypic heterogeneity	2159:2182	phenotypic heterogeneity	2159:2182	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	11	62	theme	labor	2211:2215	arg1	division					2199:2206	supporting division	2188:2206	supporting division	2188:2206	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	6	63	theme	RsaE	1251:1254	arg1	processing					1256:1265	RsaE processing	1251:1265	RsaE processing	1251:1265	RsaE processing results in expansion of the mRNA target spectrum.
30870530	4	64	theme	PIA-mediated	893:904	arg1	biofilms					906:913	S. epidermidis PIA-mediated biofilms	878:913	S. epidermidis PIA-mediated biofilms	878:913	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	3	65	theme	matrix	760:765	arg1	producers					767:775	S. epidermidis protein- and PIA-biofilm matrix producers	720:775	producers	767:775	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	5	66	from	5'-end	1243:1248	arg1	nucleotides					1224:1234	approximately 20 nucleotides	1207:1234	approximately 20 nucleotides at the 5'-end	1207:1248	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	11	67	theme	biofilm	2235:2241	arg1	communities					2243:2253	S. epidermidis biofilm communities	2220:2253	S. epidermidis biofilm communities	2220:2253	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	9	68	theme	biofilm	1682:1688	arg1	production					1697:1706	PIA-mediated biofilm matrix production	1669:1706	PIA-mediated biofilm matrix production	1669:1706	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	11	69	theme	phenotypic	2159:2168	arg1	heterogeneity					2170:2182	phenotypic heterogeneity	2159:2182	phenotypic heterogeneity	2159:2182	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	8	70	theme	mRNAs	1516:1520	arg1	5'-UTR					1491:1496	the 5'-UTR	1487:1496	the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively	1487:1652	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	5	71	theme	major	1133:1137	arg1	transcript					1158:1167	a major full-length 100-nt transcript	1131:1167	a major full-length 100-nt transcript	1131:1167	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	4	72	theme	eDNA	983:986	arg1	release					988:994	eDNA release	983:994	a PIA biofilm phenotype as well as eDNA release	948:994	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	6	73	theme	target	1300:1305	arg1	spectrum					1307:1314	the mRNA target spectrum	1291:1314	the mRNA target spectrum	1291:1314	RsaE processing results in expansion of the mRNA target spectrum.
30870530	5	74	theme	100-nt	1151:1156	arg1	transcript					1158:1167	a major full-length 100-nt transcript	1131:1167	a major full-length 100-nt transcript	1131:1167	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	4	75	theme	PIA	950:952	arg1	phenotype					962:970	a PIA biofilm phenotype	948:970	a PIA biofilm phenotype as well as eDNA release	948:994	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	8	76	theme	icaADBC	1536:1542	arg1	repressor					1559:1567	the icaADBC biofilm operon repressor	1532:1567	the icaADBC biofilm operon repressor IcaR	1532:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	8	76	theme	icaADBC	1536:1542	arg1	IcaR					1569:1572	IcaR	1569:1572	IcaR	1569:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	3	77	from	sequencing	593:602	arg1	combination					618:628	combination	618:628	combination with confocal laser scanning microscopy (CLSM)	618:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	78	theme	metabolic	809:817	arg1	expression					824:833	metabolic gene expression	809:833	metabolic gene expression	809:833	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	1	79	from	riboregulator	218:230	arg1	aureus					302:307	Staphylococcus aureus	287:307	Staphylococcus aureus	287:307	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	79	from	riboregulator	218:230	arg1	subtilis					322:329	Bacillus subtilis	313:329	Bacillus subtilis	313:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	80	theme	conserved	135:143	arg1	RNA					162:164	small regulatory RNA	145:164	a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis	133:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	80	theme	conserved	135:143	arg1	RsaE					125:128	RsaE	125:128	RsaE	125:128	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	80	theme	conserved	135:143	arg1	sRNA					167:170	sRNA	167:170	sRNA	167:170	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	9	81	theme	TCA	1806:1808	arg1	inhibition					1816:1825	TCA cycle inhibition	1806:1825	TCA cycle inhibition	1806:1825	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	8	82	theme	operon	1552:1557	arg1	repressor					1559:1567	the icaADBC biofilm operon repressor	1532:1567	the icaADBC biofilm operon repressor IcaR	1532:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	8	82	theme	operon	1552:1557	arg1	IcaR					1569:1572	IcaR	1569:1572	IcaR	1569:1572	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	3	83	theme	laser	644:648	arg1	CLSM					671:674	CLSM	671:674	CLSM	671:674	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	83	theme	laser	644:648	arg1	microscopy					659:668	confocal laser scanning microscopy	635:668	confocal laser scanning microscopy (CLSM)	635:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	1	84	theme	regulatory	151:160	arg1	RNA					162:164	small regulatory RNA	145:164	a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis	133:329	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	84	theme	regulatory	151:160	arg1	RsaE					125:128	RsaE	125:128	RsaE	125:128	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	1	84	theme	regulatory	151:160	arg1	sRNA					167:170	sRNA	167:170	sRNA	167:170	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
30870530	11	85	gly	heterogeneity	2170:2182	arg1	labor					2211:2215	labor	2211:2215	labor	2211:2215	As RsaE itself is heterogeneously expressed within biofilms, we consider this sRNA to function as a factor favoring phenotypic heterogeneity and supporting division of labor in S. epidermidis biofilm communities.
30870530	2	86	theme	hypervariable	495:507	arg1	isolate					536:542	a hypervariable Staphylococcus epidermidis isolate	493:542	a hypervariable Staphylococcus epidermidis isolate	493:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	7	87	theme	eDNA	1433:1436	arg1	release					1438:1444	eDNA release	1433:1444	eDNA release	1433:1444	Thus, full-length RsaE interacts with S. epidermidis antiholin-encoding lrgA mRNA, facilitating bacterial lysis and eDNA release.
30870530	3	88	with	combination	618:628	arg1	CLSM					671:674	CLSM	671:674	CLSM	671:674	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	3	88	with	combination	618:628	arg1	microscopy					659:668	confocal laser scanning microscopy	635:668	confocal laser scanning microscopy (CLSM)	635:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	4	89	theme	matrix-producing	1033:1048	arg1	background					1057:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	8	90	theme	succinyl-CoA	1578:1589	arg1	synthetase					1591:1600	succinyl-CoA synthetase	1578:1600	succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle	1578:1638	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	9	91	theme	staphylococcal	1849:1862	arg1	metabolism					1879:1888	staphylococcal central carbon metabolism	1849:1888	staphylococcal central carbon metabolism	1849:1888	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	7	92	theme	bacterial	1413:1421	arg1	lysis					1423:1427	bacterial lysis	1413:1427	bacterial lysis	1413:1427	Thus, full-length RsaE interacts with S. epidermidis antiholin-encoding lrgA mRNA, facilitating bacterial lysis and eDNA release.
30870530	4	93	theme	S.	1002:1003	arg1	background					1057:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	an S. epidermidis protein biofilm matrix-producing strain background	999:1066	RsaE is spatiotemporally expressed within S. epidermidis PIA-mediated biofilms, and its overexpression triggers a PIA biofilm phenotype as well as eDNA release in an S. epidermidis protein biofilm matrix-producing strain background.
30870530	2	94	from	production	479:488	arg1	isolate					536:542	a hypervariable Staphylococcus epidermidis isolate	493:542	a hypervariable Staphylococcus epidermidis isolate	493:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	0	95	theme	small	4:8	arg1	RsaE					25:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	5	96	theme	minor	1175:1179	arg1	species					1191:1197	a minor processed species	1173:1197	a minor processed species lacking approximately 20 nucleotides at the 5'-end	1173:1248	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	2	97	theme	intercellular	451:463	arg1	production					479:488	polysaccharide intercellular adhesin (PIA) production	436:488	polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate	436:542	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	8	98	theme	Processed	1447:1455	arg1	RsaE					1457:1460	Processed RsaE	1447:1460	Processed RsaE	1447:1460	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	0	99	theme	RNA	21:23	arg1	RsaE					25:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE	0:28	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	7	100	theme	lrgA	1389:1392	arg1	mRNA					1394:1397	lrgA mRNA	1389:1397	lrgA mRNA	1389:1397	Thus, full-length RsaE interacts with S. epidermidis antiholin-encoding lrgA mRNA, facilitating bacterial lysis and eDNA release.
30870530	9	101	theme	precursor	1902:1910	arg1	synthesis					1912:1920	PIA precursor synthesis	1898:1920	PIA precursor synthesis	1898:1920	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	2	102	theme	biofilm-matrix	403:416	arg1	switching					418:426	biofilm-matrix switching	403:426	biofilm-matrix switching	403:426	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	5	103	theme	Northern	1082:1089	arg1	analyses					1096:1103	dRNA-seq and Northern blot analyses	1069:1103	analyses	1096:1103	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	9	104	theme	carbon	1872:1877	arg1	metabolism					1879:1888	staphylococcal central carbon metabolism	1849:1888	staphylococcal central carbon metabolism	1849:1888	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	0	105	theme	matrix	55:60	arg1	composition					62:72	extracellular matrix composition	41:72	extracellular matrix composition in Staphylococcus epidermidis biofilm communities	41:122	The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.
30870530	9	106	theme	PIA-mediated	1669:1680	arg1	production					1697:1706	PIA-mediated biofilm matrix production	1669:1706	PIA-mediated biofilm matrix production	1669:1706	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	8	107	theme	tricarboxylic	1609:1621	arg1	cycle					1634:1638	the tricarboxylic acid (TCA) cycle	1605:1638	the tricarboxylic acid (TCA) cycle	1605:1638	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	2	108	dep	Staphylococcus	509:522	arg1	epidermidis					524:534	epidermidis	524:534	epidermidis	524:534	Here we show that RsaE contributes to extracellular (e)DNA release and biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production in a hypervariable Staphylococcus epidermidis isolate.
30870530	9	109	theme	matrix	1690:1695	arg1	production					1697:1706	PIA-mediated biofilm matrix production	1669:1706	PIA-mediated biofilm matrix production	1669:1706	RsaE augments PIA-mediated biofilm matrix production, most likely through activation of icaADBC operon expression via repression of icaR as well as by TCA cycle inhibition and re-programming of staphylococcal central carbon metabolism towards PIA precursor synthesis.
30870530	3	110	theme	gene	690:693	arg1	fusions					695:701	reporter gene fusions	681:701	reporter gene fusions	681:701	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	5	111	theme	dRNA-seq	1069:1076	arg1	analyses					1096:1103	dRNA-seq and Northern blot analyses	1069:1103	analyses	1096:1103	dRNA-seq and Northern blot analyses revealed RsaE to exist as a major full-length 100-nt transcript and a minor processed species lacking approximately 20 nucleotides at the 5'-end.
30870530	10	112	theme	stabilizing	2005:2015	arg1	component					2032:2040	stabilizing biofilm matrix component	2005:2040	stabilizing biofilm matrix component	2005:2040	Additionally, RsaE supports biofilm formation by mediating the release of eDNA as stabilizing biofilm matrix component.
30870530	8	113	theme	icaR	1501:1504	arg1	mRNAs					1516:1520	icaR and sucCD mRNAs	1501:1520	mRNAs	1516:1520	Processed RsaE, however, interacts with the 5'-UTR of icaR and sucCD mRNAs, encoding the icaADBC biofilm operon repressor IcaR and succinyl-CoA synthetase of the tricarboxylic acid (TCA) cycle, respectively.
30870530	3	114	theme	Transcriptome	545:557	arg1	analysis					559:566	Transcriptome analysis	545:566	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM)	545:675	Transcriptome analysis through differential RNA sequencing (dRNA-seq) in combination with confocal laser scanning microscopy (CLSM) and reporter gene fusions demonstrate that S. epidermidis protein- and PIA-biofilm matrix producers differ with respect to RsaE and metabolic gene expression.
30870530	1	115	theme	carbon	243:248	arg1	flow					250:253	central carbon flow	235:253	central carbon flow	235:253	RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent a riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus and Bacillus subtilis.
31071405	1	0	theme	electrospraying	393:407	arg1	technique					409:417	electrospraying technique	393:417	electrospraying technique	393:417	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	1	1	theme	fibrous	179:185	arg1	membranes					187:195	fibrous membranes	179:195	fibrous membranes	179:195	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	5	2	with	Modification	928:939	arg1	nanoparticles					973:985	the hydrogel nanoparticles	960:985	the hydrogel nanoparticles	960:985	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	6	3	with	culturing	1150:1158	arg1	cells					1193:1197	L929 fibroblast cells	1177:1197	L929 fibroblast cells	1177:1197	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	1	4	theme	membranes	187:195	arg1	properties					149:158	physicomechanical properties	131:158	physicomechanical properties	131:158	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	1	4	theme	membranes	187:195	arg1	bioactivity					164:174	bioactivity	164:174	bioactivity of fibrous membranes for wound dressing and tissue engineering applications	164:250	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	1	5	theme	fibrous	293:299	arg1	mats					301:304	fibrous mats	293:304	fibrous mats	293:304	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	6	6	theme	composite	1246:1254	arg1	biocompatibility					1214:1229	the biocompatibility	1210:1229	the biocompatibility of the fibrous composite	1210:1254	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	6	7	theme	improved	1267:1274	arg1	adhesion					1281:1288	improved cell adhesion	1267:1288	improved cell adhesion	1267:1288	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	0	8	theme	wound	105:109	arg1	healing					111:117	wound healing	105:117	wound healing	105:117	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	5	9	theme	scaffold	1131:1138	arg1	%					1033:1033	~23%	1030:1033	~23%	1030:1033	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	ductility					1095:1103	ductility	1095:1103	ductility (~23%) of the electrospun scaffold	1095:1138	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	%					1109:1109	~23%	1106:1109	~23%	1106:1109	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	%					1087:1087	~35%	1084:1087	~35%	1084:1087	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	hydrophilicity					1014:1027	the hydrophilicity	1010:1027	the hydrophilicity (~23%)	1010:1034	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	module					1037:1042	module	1037:1042	module of elasticity (~31%)	1037:1063	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	9	theme	scaffold	1131:1138	arg1	strength					1074:1081	tensile strength	1066:1081	tensile strength (~35%)	1066:1088	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	2	10	theme	composite	424:432	arg1	scaffolds					434:442	The composite scaffolds	420:442	The composite scaffolds	420:442	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	11	theme	nonionic	581:588	arg1	copolymers					599:608	nonionic triblock copolymers	581:608	nonionic triblock copolymers conjugated with Tragacanth gum (TG)	581:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	4	12	from	junctions	856:864	arg1	particles					792:800	The hydrogel particles	779:800	The hydrogel particles	779:800	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	4	12	from	junctions	856:864	arg1	embedded					817:824	embedded	817:824	embedded	817:824	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	2	13	theme	cellulose	479:487	arg1	150 ± 43 nm					497:507	150 ± 43 nm	497:507	150 ± 43 nm	497:507	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	13	theme	cellulose	479:487	arg1	fibers					489:494	keratin/bacterial cellulose fibers	461:494	keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG)	461:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	1	14	theme	wound	201:205	arg1	dressing					207:214	wound dressing	201:214	wound dressing	201:214	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	1	15	theme	dressing	207:214	arg1	applications					239:250	wound dressing and tissue engineering applications	201:250	wound dressing and tissue engineering applications	201:250	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	1	16	theme	thermosensitive	310:324	arg1	particles					335:343	thermosensitive hydrogel particles	310:343	thermosensitive hydrogel particles	310:343	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	4	17	theme	fibrous	831:837	arg1	network					839:845	fibrous network	831:845	fibrous network	831:845	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	0	18	theme	cellulose/keratin	26:42	arg1	nanofibrous					44:54	bacterial cellulose/keratin nanofibrous	16:54	bacterial cellulose/keratin nanofibrous	16:54	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	1	19	theme	hydrogel	326:333	arg1	particles					335:343	thermosensitive hydrogel particles	310:343	thermosensitive hydrogel particles	310:343	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	6	20	dep	In	1141:1142	arg1	vitro					1144:1148	vitro	1144:1148	vitro	1144:1148	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	4	21	theme	fiber	850:854	arg1	junctions					856:864	fiber junctions	850:864	fiber junctions	850:864	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	1	22	theme	tissue	220:225	arg1	engineering					227:237	tissue engineering	220:237	tissue engineering	220:237	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	0	23	theme	bacterial	16:24	arg1	nanofibrous					44:54	bacterial cellulose/keratin nanofibrous	16:54	bacterial cellulose/keratin nanofibrous	16:54	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	2	24	theme	Tragacanth	626:635	arg1	TG					642:643	TG	642:643	TG	642:643	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	24	theme	Tragacanth	626:635	arg1	gum					637:639	Tragacanth gum	626:639	Tragacanth gum (TG)	626:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	4	25	theme	hydrogel	783:790	arg1	particles					792:800	The hydrogel particles	779:800	The hydrogel particles	779:800	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	4	25	theme	hydrogel	783:790	arg1	embedded					817:824	embedded	817:824	embedded	817:824	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	1	26	theme	engineering	227:237	arg1	applications					239:250	wound dressing and tissue engineering applications	201:250	wound dressing and tissue engineering applications	201:250	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	2	27	theme	500 nm	556:561	arg1	particles					545:553	hydrogel particles	536:553	hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG)	536:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	27	theme	500 nm	556:561	arg1	2 μm					566:569	500 nm to 2 μm	556:569	500 nm to 2 μm	556:569	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	3	28	theme	H-NMR	656:660	arg1	studies					662:668	FTIR and H-NMR studies	647:668	studies	662:668	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	5	29	theme	fibers	948:953	arg1	Modification					928:939	Modification	928:939	Modification of the fibers with the hydrogel nanoparticles	928:985	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	0	30	theme	nanofibrous	44:54	arg1	Modification					0:11	Modification	0:11	Modification of bacterial cellulose/keratin nanofibrous	0:54	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	5	31	theme	hydrogel	964:971	arg1	nanoparticles					973:985	the hydrogel nanoparticles	960:985	the hydrogel nanoparticles	960:985	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	3	32	theme	carboxylated	704:715	arg1	copolymers					717:726	carboxylated copolymers	704:726	carboxylated copolymers	704:726	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	4	33	theme	fiber	912:916	arg1	diameter					918:925	the fiber diameter	908:925	the fiber diameter	908:925	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	2	34	dep	2 μm	566:569	arg1	to					563:564	to	563:564	to	563:564	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	5	35	theme	electrospun	1119:1129	arg1	scaffold					1131:1138	the electrospun scaffold	1115:1138	the electrospun scaffold	1115:1138	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	3	36	theme	FTIR	647:650	arg1	studies					662:668	FTIR and H-NMR studies	647:668	studies	662:668	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	6	37	theme	mats	1167:1170	arg1	culturing					1150:1158	In vitro culturing	1141:1158	In vitro culturing of the mats with L929 fibroblast cells	1141:1197	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	2	38	theme	hydrogel	536:543	arg1	particles					545:553	hydrogel particles	536:553	hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG)	536:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	38	theme	hydrogel	536:543	arg1	2 μm					566:569	500 nm to 2 μm	556:569	500 nm to 2 μm	556:569	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	1	39	theme	concurrent	362:371	arg1	electrospinning					373:387	concurrent electrospinning	362:387	concurrent electrospinning	362:387	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	3	40	theme	crosslinker	756:766	arg1	chemistry					768:776	carbodiimide crosslinker chemistry	743:776	carbodiimide crosslinker chemistry	743:776	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	6	41	theme	L929	1177:1180	arg1	cells					1193:1197	L929 fibroblast cells	1177:1197	L929 fibroblast cells	1177:1197	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	1	42	theme	physicomechanical	131:147	arg1	properties					149:158	physicomechanical properties	131:158	physicomechanical properties	131:158	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	5	43	theme	tensile	1066:1072	arg1	strength					1074:1081	tensile strength	1066:1081	tensile strength (~35%)	1066:1088	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	43	theme	tensile	1066:1072	arg1	%					1087:1087	~35%	1084:1087	~35%	1084:1087	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	6	44	theme	cell	1276:1279	arg1	adhesion					1281:1288	improved cell adhesion	1267:1288	improved cell adhesion	1267:1288	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	5	45	theme	elasticity	1047:1056	arg1	%					1033:1033	~23%	1030:1033	~23%	1030:1033	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	ductility					1095:1103	ductility	1095:1103	ductility (~23%) of the electrospun scaffold	1095:1138	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	%					1109:1109	~23%	1106:1109	~23%	1106:1109	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	%					1087:1087	~35%	1084:1087	~35%	1084:1087	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	hydrophilicity					1014:1027	the hydrophilicity	1010:1027	the hydrophilicity (~23%)	1010:1034	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	module					1037:1042	module	1037:1042	module of elasticity (~31%)	1037:1063	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	5	45	theme	elasticity	1047:1056	arg1	strength					1074:1081	tensile strength	1066:1081	tensile strength (~35%)	1066:1088	Modification of the fibers with the hydrogel nanoparticles significantly improved the hydrophilicity (~23%), module of elasticity (~31%), tensile strength (~35%), and ductility (~23%) of the electrospun scaffold.
31071405	1	46	theme	novel	253:257	arg1	scaffolds					269:277	novel composite scaffolds	253:277	novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles	253:343	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	4	47	theme	porous	887:892	arg1	structure					894:902	its porous structure	883:902	its porous structure	883:902	The hydrogel particles were uniformly embedded into fibrous network at fiber junctions without changing its porous structure and the fiber diameter.
31071405	1	48	theme	composite	259:267	arg1	scaffolds					269:277	novel composite scaffolds	253:277	novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles	253:343	To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique.
31071405	0	49	theme	gum-conjugated	77:90	arg1	hydrogel					92:99	a tragacanth gum-conjugated hydrogel	64:99	a tragacanth gum-conjugated hydrogel for wound healing	64:117	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	3	50	theme	carbodiimide	743:754	arg1	chemistry					768:776	carbodiimide crosslinker chemistry	743:776	carbodiimide crosslinker chemistry	743:776	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	6	51	theme	fibrous	1238:1244	arg1	composite					1246:1254	the fibrous composite	1234:1254	the fibrous composite	1234:1254	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	2	52	theme	keratin/bacterial	461:477	arg1	150 ± 43 nm					497:507	150 ± 43 nm	497:507	150 ± 43 nm	497:507	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	2	52	theme	keratin/bacterial	461:477	arg1	fibers					489:494	keratin/bacterial cellulose fibers	461:494	keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG)	461:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
31071405	6	53	theme	fibroblast	1182:1191	arg1	cells					1193:1197	L929 fibroblast cells	1177:1197	L929 fibroblast cells	1177:1197	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	0	54	theme	tragacanth	66:75	arg1	hydrogel					92:99	a tragacanth gum-conjugated hydrogel	64:99	a tragacanth gum-conjugated hydrogel for wound healing	64:117	Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
31071405	3	55	theme	ester	680:684	arg1	reactions					686:694	ester reactions	680:694	ester reactions between carboxylated copolymers and TG	680:733	FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry.
31071405	6	56	theme	In	1141:1142	arg1	culturing					1150:1158	In vitro culturing	1141:1158	In vitro culturing of the mats with L929 fibroblast cells	1141:1197	In vitro culturing of the mats with L929 fibroblast cells determined the biocompatibility of the fibrous composite along with improved cell adhesion and proliferation.
31071405	2	57	theme	triblock	590:597	arg1	copolymers					599:608	nonionic triblock copolymers	581:608	nonionic triblock copolymers conjugated with Tragacanth gum (TG)	581:644	The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG).
30227334	0	0	theme	real	81:84	arg1	soil					86:89	real soil	81:89	real soil	81:89	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	7	1	from	heterogeneity	890:902	arg1	soil					950:953	soil	950:953	soil	950:953	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	8	2	theme	composite	1100:1108	arg1	degradation					1074:1084	the degradation	1070:1084	the degradation of starch/PLA composite	1070:1108	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	8	2	theme	composite	1100:1108	arg1	degradation					1119:1129	bulk degradation	1114:1129	bulk degradation	1114:1129	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	1	3	theme	waste	151:155	arg1	disposal					157:164	the waste disposal	147:164	the waste disposal	147:164	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	1	3	theme	waste	151:155	arg1	challenge					171:179	a challenge	169:179	a challenge in this case	169:192	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	7	4	gly	heterogeneity	890:902	arg1	biodegradation					907:920	biodegradation	907:920	biodegradation for starch/PLA composite	907:945	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	8	5	theme	starch/PLA	1089:1098	arg1	composite					1100:1108	starch/PLA composite	1089:1108	starch/PLA composite	1089:1108	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	7	6	theme	percent	868:874	arg1	distribution					809:820	The distribution	805:820	The distribution of carboxyl group intensity and carbon atomic percent	805:874	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	6	7	theme	starch/PLA	734:743	arg1	composite					745:753	the starch/PLA composite	730:753	the starch/PLA composite	730:753	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	8	8	theme	atomic	999:1004	arg1	percent					1006:1012	internal carbon atomic percent	983:1012	internal carbon atomic percent	983:1012	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	9	9	theme	interested	1308:1317	arg1	people					1301:1306	people	1301:1306	people interested in studying the biodegradation of PLA	1301:1355	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	1	10	from	problem	135:141	arg1	case					189:192	this case	184:192	this case	184:192	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	9	11	theme	biodegradation	1407:1420	arg1	rate					1422:1425	the biodegradation rate	1403:1425	the biodegradation rate of PLA	1403:1432	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	2	12	theme	attractive	269:278	arg1	polymers					280:287	the most attractive polymers	260:287	the most attractive polymers which can fulfill the current demand	260:324	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	5	13	theme	starch	670:675	arg1	presence					658:665	the presence	654:665	the presence of starch	654:675	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	8	14	theme	carbon	992:997	arg1	percent					1006:1012	internal carbon atomic percent	983:1012	internal carbon atomic percent	983:1012	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	5	15	dep	Fourier	567:573	arg1	transform					575:583	transform	575:583	transform infrared (FTIR) results	575:607	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	9	16	from	content	1456:1462	arg1	composite					1481:1489	the composite	1477:1489	the composite	1477:1489	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	8	17	theme	internal	983:990	arg1	percent					1006:1012	internal carbon atomic percent	983:1012	internal carbon atomic percent	983:1012	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	7	18	theme	atomic	861:866	arg1	percent					868:874	carbon atomic percent	854:874	carbon atomic percent	854:874	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	8	19	theme	bulk	1114:1117	arg1	degradation					1074:1084	the degradation	1070:1084	the degradation of starch/PLA composite	1070:1108	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	8	19	theme	bulk	1114:1117	arg1	degradation					1119:1129	bulk degradation	1114:1129	bulk degradation	1114:1129	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	4	20	theme	weight	428:433	arg1	loss					435:438	The weight loss	424:438	The weight loss	424:438	The weight loss results demonstrated that the degradation rate of PLA could be accelerated by starch.
30227334	8	21	theme	percent	1006:1012	arg1	variation					970:978	the variation	966:978	the variation of internal carbon atomic percent	966:1012	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	8	21	theme	percent	1006:1012	arg1	higher					1018:1023	higher	1018:1023	higher	1018:1023	Moreover, the variation of internal carbon atomic percent was higher than that on the surface, demonstrating that the degradation of starch/PLA composite was bulk degradation.
30227334	7	22	theme	carbon	854:859	arg1	percent					868:874	carbon atomic percent	854:874	carbon atomic percent	854:874	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	3	23	theme	starch/PLA	360:369	arg1	composite					371:379	starch/PLA composite	360:379	starch/PLA composite	360:379	In this work, the degradation of starch/PLA composite was investigated in real soil environment.
30227334	0	24	theme	Physicochemical	0:14	arg1	evolutions					16:25	Physicochemical evolutions	0:25	Physicochemical evolutions of starch/poly (lactic acid) composite	0:64	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	5	25	theme	infrared	585:592	arg1	results					601:607	infrared (FTIR) results	585:607	infrared (FTIR) results	585:607	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	5	26	theme	Scanning	526:533	arg1	SEM					558:560	SEM	558:560	SEM	558:560	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	5	26	theme	Scanning	526:533	arg1	microscopy					546:555	Scanning electrical microscopy	526:555	Scanning electrical microscopy (SEM)	526:561	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	3	27	theme	composite	371:379	arg1	degradation					345:355	the degradation	341:355	the degradation of starch/PLA composite	341:379	In this work, the degradation of starch/PLA composite was investigated in real soil environment.
30227334	0	28	theme	starch/poly	30:40	arg1	composite					56:64	starch/poly (lactic acid) composite	30:64	starch/poly (lactic acid) composite	30:64	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	6	29	theme	abrupt	710:715	arg1	decrease					717:724	an abrupt decrease	707:724	an abrupt decrease for the starch/PLA composite	707:753	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	5	30	theme	electrical	535:544	arg1	SEM					558:560	SEM	558:560	SEM	558:560	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	5	30	theme	electrical	535:544	arg1	microscopy					546:555	Scanning electrical microscopy	526:555	Scanning electrical microscopy (SEM)	526:561	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	1	31	theme	Plastic	92:98	arg1	problem					135:141	a major environmental problem	113:141	a major environmental problem	113:141	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	1	31	theme	Plastic	92:98	arg1	pollution					100:108	Plastic pollution	92:108	Plastic pollution	92:108	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	3	32	theme	soil	406:409	arg1	environment					411:421	real soil environment	401:421	real soil environment	401:421	In this work, the degradation of starch/PLA composite was investigated in real soil environment.
30227334	6	33	contain	had	703:705	arg1	strengths					693:701	The mechanical strengths	678:701	The mechanical strengths	678:701	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	6	33	contain	had	703:705	arg2	decrease					717:724	an abrupt decrease	707:724	an abrupt decrease for the starch/PLA composite	707:753	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	6	34	theme	low	793:795	arg1	degree					797:802	a low degree	791:802	a low degree	791:802	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	0	35	theme	lactic	43:48	arg1	acid					50:53	lactic acid	43:53	lactic acid	43:53	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	9	36	theme	scientific	1280:1289	arg1	basis					1291:1295	a scientific basis	1278:1295	a scientific basis for people interested in studying the biodegradation of PLA	1278:1355	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	5	37	dep	infrared	585:592	arg1	FTIR					595:598	FTIR	595:598	FTIR	595:598	Scanning electrical microscopy (SEM) and Fourier transform infrared (FTIR) results showed that the samples degraded faster with the presence of starch.
30227334	2	38	theme	lactic	201:206	arg1	Poly					195:198	Poly	195:198	Poly (lactic acid) (PLA) based biodegradable materials	195:248	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	2	38	theme	lactic	201:206	arg1	acid					208:211	lactic acid	201:211	lactic acid	201:211	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	3	39	theme	real	401:404	arg1	environment					411:421	real soil environment	401:421	real soil environment	401:421	In this work, the degradation of starch/PLA composite was investigated in real soil environment.
30227334	9	40	theme	physicochemical	1199:1213	arg1	evolutions					1227:1236	the physicochemical performance evolutions	1195:1236	the physicochemical performance evolutions	1195:1236	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	4	41	theme	degradation	470:480	arg1	rate					482:485	the degradation rate	466:485	the degradation rate of PLA	466:492	The weight loss results demonstrated that the degradation rate of PLA could be accelerated by starch.
30227334	9	42	theme	starch/PLA	1170:1179	arg1	composite					1181:1189	starch/PLA composite	1170:1189	starch/PLA composite	1170:1189	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	2	43	theme	current	311:317	arg1	demand					319:324	the current demand	307:324	the current demand	307:324	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	4	44	theme	PLA	490:492	arg1	rate					482:485	the degradation rate	466:485	the degradation rate of PLA	466:492	The weight loss results demonstrated that the degradation rate of PLA could be accelerated by starch.
30227334	6	45	theme	mechanical	682:691	arg1	strengths					693:701	The mechanical strengths	678:701	The mechanical strengths	678:701	The mechanical strengths had an abrupt decrease for the starch/PLA composite while that of PLA only decreased in a low degree.
30227334	7	46	theme	group	834:838	arg1	intensity					840:848	carboxyl group intensity	825:848	carboxyl group intensity	825:848	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	4	47	dep	results	440:446	arg1	demonstrated					448:459	demonstrated	448:459	results demonstrated that the degradation rate of PLA could be accelerated by starch	440:523	The weight loss results demonstrated that the degradation rate of PLA could be accelerated by starch.
30227334	0	48	dep	starch/poly	30:40	arg1	acid					50:53	lactic acid	43:53	lactic acid	43:53	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	9	49	theme	starch	1467:1472	arg1	content					1456:1462	the content	1452:1462	the content of starch in the composite	1452:1489	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	1	50	theme	major	115:119	arg1	problem					135:141	a major environmental problem	113:141	a major environmental problem	113:141	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	1	50	theme	major	115:119	arg1	pollution					100:108	Plastic pollution	92:108	Plastic pollution	92:108	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	7	51	theme	intensity	840:848	arg1	distribution					809:820	The distribution	805:820	The distribution of carboxyl group intensity and carbon atomic percent	805:874	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	7	52	theme	starch/PLA	926:935	arg1	composite					937:945	starch/PLA composite	926:945	starch/PLA composite	926:945	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	1	53	theme	environmental	121:133	arg1	problem					135:141	a major environmental problem	113:141	a major environmental problem	113:141	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	1	53	theme	environmental	121:133	arg1	pollution					100:108	Plastic pollution	92:108	Plastic pollution	92:108	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	2	54	theme	polymers	280:287	arg1	polymers					280:287	the most attractive polymers	260:287	the most attractive polymers which can fulfill the current demand	260:324	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	2	54	theme	polymers	280:287	arg1	one					253:255	one	253:255	one	253:255	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	9	55	theme	PLA	1430:1432	arg1	rate					1422:1425	the biodegradation rate	1403:1425	the biodegradation rate of PLA	1403:1432	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	9	56	theme	starch	1153:1158	arg1	role					1145:1148	the role	1141:1148	the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation	1141:1258	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	1	57	from	challenge	171:179	arg1	case					189:192	this case	184:192	this case	184:192	Plastic pollution is a major environmental problem and the waste disposal is a challenge in this case.
30227334	7	58	theme	carboxyl	825:832	arg1	intensity					840:848	carboxyl group intensity	825:848	carboxyl group intensity	825:848	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	0	59	theme	composite	56:64	arg1	evolutions					16:25	Physicochemical evolutions	0:25	Physicochemical evolutions of starch/poly (lactic acid) composite	0:64	Physicochemical evolutions of starch/poly (lactic acid) composite biodegraded in real soil.
30227334	9	60	theme	performance	1215:1225	arg1	evolutions					1227:1236	the physicochemical performance evolutions	1195:1236	the physicochemical performance evolutions	1195:1236	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30227334	7	61	theme	biodegradation	907:920	arg1	heterogeneity					890:902	the heterogeneity	886:902	the heterogeneity of biodegradation for starch/PLA composite in soil	886:953	The distribution of carboxyl group intensity and carbon atomic percent reflected the heterogeneity of biodegradation for starch/PLA composite in soil.
30227334	2	62	dep	based	220:224	arg1	materials					240:248	materials	240:248	materials	240:248	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	2	62	dep	based	220:224	arg1	Poly					195:198	Poly	195:198	Poly (lactic acid) (PLA) based biodegradable materials	195:248	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	2	62	dep	based	220:224	arg1	biodegradable					226:238	biodegradable	226:238	biodegradable	226:238	Poly (lactic acid) (PLA) based biodegradable materials is one of the most attractive polymers which can fulfill the current demand.
30227334	9	63	theme	PLA	1353:1355	arg1	biodegradation					1335:1348	the biodegradation	1331:1348	the biodegradation of PLA	1331:1355	Based on the role of starch played in starch/PLA composite and the physicochemical performance evolutions during biodegradation, it should create a scientific basis for people interested in studying the biodegradation of PLA, and provide some knowledge about controlling the biodegradation rate of PLA through adjusting the content of starch in the composite.
30184743	4	0	theme	study	582:586	arg1	Findings					566:573	Findings	566:573	Findings of the study	566:586	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	9	1	theme	predicted	1200:1208	arg1	conditions					1235:1244	The predicted appropriate drug release conditions	1196:1244	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH	1196:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	1	2	theme	modified	163:170	arg1	hydrogel					189:196	modified acrylamide-based hydrogel	163:196	modified acrylamide-based hydrogel	163:196	This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites.
30184743	10	3	theme	desired	1423:1429	arg1	value					1431:1435	the desired value	1419:1435	the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g)	1419:1479	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	8	4	theme	drug	1143:1146	arg1	release					1148:1154	the drug release	1139:1154	the drug release	1139:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	0	5	theme	surface	101:107	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.
30184743	9	6	theme	temperature	1383:1393	arg1	38 °C					1395:1399	temperature 38 °C	1383:1399	temperature 38 °C	1383:1399	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	10	7	theme	MgO	1440:1442	arg1	value					1431:1435	the desired value	1419:1435	the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g)	1419:1479	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	0	8	theme	response	92:99	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.
30184743	9	9	theme	drug	1222:1225	arg1	conditions					1235:1244	The predicted appropriate drug release conditions	1196:1244	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH	1196:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	9	10	from	rate	1278:1281	arg1	hydrogel					1254:1261	the hydrogel	1250:1261	the hydrogel at the highest rate of temperature (37.50 °C) and pH	1250:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	9	11	theme	release	1227:1233	arg1	conditions					1235:1244	The predicted appropriate drug release conditions	1196:1244	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH	1196:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	7	12	theme	variables	1040:1048	arg1	effects					1025:1031	the effects	1021:1031	the effects of two variables, i.e. MgO and NaCMC	1021:1068	With the swelling ratio for acrylamide-based hydrogel as the response, the effects of two variables, i.e. MgO and NaCMC were investigated.
30184743	6	13	theme	surface	786:792	arg1	methodology					794:804	Response surface methodology	777:804	Response surface methodology (RSM) based on a central composite design (CCD Design)	777:859	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	6	13	theme	surface	786:792	arg1	RSM					807:809	RSM	807:809	RSM	807:809	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	1	14	theme	acrylamide-based	172:187	arg1	hydrogel					189:196	modified acrylamide-based hydrogel	163:196	modified acrylamide-based hydrogel	163:196	This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites.
30184743	8	15	theme	pH	1104:1105	arg1	effects					1093:1099	The effects	1089:1099	The effects of pH, temperature, MgO, and NaCMC on the drug release	1089:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	8	16	theme	NaCMC	1130:1134	arg1	effects					1093:1099	The effects	1089:1099	The effects of pH, temperature, MgO, and NaCMC on the drug release	1089:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	6	17	theme	Response	777:784	arg1	methodology					794:804	Response surface methodology	777:804	Response surface methodology (RSM) based on a central composite design (CCD Design)	777:859	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	6	17	theme	Response	777:784	arg1	RSM					807:809	RSM	807:809	RSM	807:809	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	6	18	dep	CCD	849:851	arg1	Design					853:858	Design	853:858	CCD Design	849:858	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	1	19	theme	hydrogel	189:196	arg1	structure					150:158	the structure	146:158	the structure of modified acrylamide-based hydrogel	146:196	This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites.
30184743	2	20	theme	sodium	332:337	arg1	NaCMC					364:368	NaCMC	364:368	NaCMC	364:368	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	20	theme	sodium	332:337	arg1	cellulose					353:361	sodium carboxymethyl cellulose	332:361	sodium carboxymethyl cellulose (NaCMC)	332:369	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	7	21	dep	MgO	1056:1058	arg1	i.e.					1051:1054	i.e.	1051:1054	i.e.	1051:1054	With the swelling ratio for acrylamide-based hydrogel as the response, the effects of two variables, i.e. MgO and NaCMC were investigated.
30184743	10	22	theme	amount	1457:1462	arg1	value					1431:1435	the desired value	1419:1435	the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g)	1419:1479	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	9	23	theme	highest	1333:1339	arg1	value					1341:1345	its highest value	1329:1345	its highest value	1329:1345	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	0	24	theme	acrylamide-based	20:35	arg1	hydrogels					37:45	acrylamide-based hydrogels	20:45	acrylamide-based hydrogels	20:45	From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.
30184743	3	25	theme	polymer	557:563	arg1	structure					540:548	the structure	536:548	the structure of the polymer	536:563	N,N,N',N'-tetramethylethylenediamine and ammonium persulfate as the initiator was applied in the structure of the polymer.
30184743	9	26	theme	temperature	1286:1296	arg1	rate					1278:1281	the highest rate	1266:1281	the highest rate of temperature (37.50 °C) and pH	1266:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	8	27	theme	MgO	1121:1123	arg1	effects					1093:1099	The effects	1089:1099	The effects of pH, temperature, MgO, and NaCMC on the drug release	1089:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	2	28	theme	oxide	385:389	arg1	nanoparticles					397:409	magnesium oxide (MgO) nanoparticles	375:409	magnesium oxide (MgO) nanoparticles	375:409	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	29	theme	acrylamide	311:320	arg1	monomers					322:329	acrylamide monomers	311:329	acrylamide monomers	311:329	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	1	30	theme	nano	218:221	arg1	composites					223:232	the nano composites	214:232	the nano composites	214:232	This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites.
30184743	5	31	theme	nano	760:763	arg1	composites					765:774	the nano composites	756:774	the nano composites	756:774	Thus, MgO is an appropriate nanoparticle to be used in the nano composites.
30184743	6	32	theme	hydrogel	916:923	arg1	variables					901:909	the preparation variables	885:909	the preparation variables of a hydrogel consisted of MgO, NaCMC	885:947	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	4	33	theme	highest	646:652	arg1	ratio					663:667	the highest swelling ratio	642:667	the highest swelling ratio	642:667	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	3	34	dep	N	443:443	arg1	persulfate					493:502	persulfate	493:502	persulfate	493:502	N,N,N',N'-tetramethylethylenediamine and ammonium persulfate as the initiator was applied in the structure of the polymer.
30184743	2	35	theme	crosslinking	414:425	arg1	polymerization					427:440	crosslinking polymerization	414:440	crosslinking polymerization	414:440	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	36	theme	nanoparticles	397:409	arg1	combination					296:306	a combination	294:306	a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles	294:409	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	9	37	theme	highest	1270:1276	arg1	rate					1278:1281	the highest rate	1266:1281	the highest rate of temperature (37.50 °C) and pH	1266:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	0	38	theme	hydrogels	37:45	arg1	formulation					5:15	formulation	5:15	formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology	5:119	From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.
30184743	6	39	theme	composite	831:839	arg1	CCD					849:851	CCD	849:851	CCD Design	849:858	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	6	39	theme	composite	831:839	arg1	design					841:846	a central composite design	821:846	a central composite design (CCD Design)	821:859	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	9	40	theme	pH	1313:1314	arg1	rate					1278:1281	the highest rate	1266:1281	the highest rate of temperature (37.50 °C) and pH	1266:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	8	41	theme	CCD	1184:1186	arg1	design					1188:1193	the CCD design	1180:1193	the CCD design	1180:1193	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	9	42	dep	conditions	1235:1244	arg1	4.10					1317:1320	4.10	1317:1320	4.10	1317:1320	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	6	43	theme	central	823:829	arg1	CCD					849:851	CCD	849:851	CCD Design	849:858	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	6	43	theme	central	823:829	arg1	design					841:846	a central composite design	821:846	a central composite design (CCD Design)	821:859	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	9	44	theme	lower	1358:1362	arg1	release					1369:1375	the lower drug release	1354:1375	the lower drug release	1354:1375	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	9	45	theme	appropriate	1210:1220	arg1	conditions					1235:1244	The predicted appropriate drug release conditions	1196:1244	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH	1196:1314	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	4	46	dep	MgO	633:635	arg1	have					637:640	have	637:640	have the highest swelling ratio compared to pure Aam hydrogels	637:698	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	9	47	theme	drug	1364:1367	arg1	release					1369:1375	the lower drug release	1354:1375	the lower drug release	1354:1375	The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50.
30184743	6	48	theme	preparation	889:899	arg1	variables					901:909	the preparation variables	885:909	the preparation variables of a hydrogel consisted of MgO, NaCMC	885:947	Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC.
30184743	7	49	theme	acrylamide-based	978:993	arg1	hydrogel					995:1002	acrylamide-based hydrogel	978:1002	acrylamide-based hydrogel as the response	978:1018	With the swelling ratio for acrylamide-based hydrogel as the response, the effects of two variables, i.e. MgO and NaCMC were investigated.
30184743	4	50	theme	pure	681:684	arg1	hydrogels					690:698	pure Aam hydrogels	681:698	pure Aam hydrogels	681:698	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	2	51	theme	cellulose	353:361	arg1	combination					296:306	a combination	294:306	a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles	294:409	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	52	theme	monomers	322:329	arg1	combination					296:306	a combination	294:306	a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles	294:409	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	7	53	theme	swelling	959:966	arg1	ratio					968:972	the swelling ratio	955:972	the swelling ratio for acrylamide-based hydrogel as the response	955:1018	With the swelling ratio for acrylamide-based hydrogel as the response, the effects of two variables, i.e. MgO and NaCMC were investigated.
30184743	8	54	theme	temperature	1108:1118	arg1	effects					1093:1099	The effects	1089:1099	The effects of pH, temperature, MgO, and NaCMC on the drug release	1089:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	2	55	theme	carboxymethyl	339:351	arg1	NaCMC					364:368	NaCMC	364:368	NaCMC	364:368	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	55	theme	carboxymethyl	339:351	arg1	cellulose					353:361	sodium carboxymethyl cellulose	332:361	sodium carboxymethyl cellulose (NaCMC)	332:369	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	4	56	theme	nano	603:606	arg1	composites					608:617	the nano composites	599:617	the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels	599:698	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	0	57	theme	drug	73:76	arg1	release					78:84	drug release	73:84	drug release using response surface methodology	73:119	From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.
30184743	8	58	from	effects	1093:1099	arg1	release					1148:1154	the drug release	1139:1154	the drug release	1139:1154	The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design.
30184743	4	59	theme	Aam	686:688	arg1	hydrogels					690:698	pure Aam hydrogels	681:698	pure Aam hydrogels	681:698	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	4	60	theme	swelling	654:661	arg1	ratio					663:667	the highest swelling ratio	642:667	the highest swelling ratio	642:667	Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels.
30184743	2	61	theme	magnesium	375:383	arg1	MgO					392:394	MgO	392:394	MgO	392:394	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	2	61	theme	magnesium	375:383	arg1	oxide					385:389	magnesium oxide	375:389	magnesium oxide (MgO) nanoparticles	375:409	The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization.
30184743	10	62	theme	NaCMC	1467:1471	arg1	0.1 g					1474:1478	0.1 g	1474:1478	0.1 g	1474:1478	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	10	62	theme	NaCMC	1467:1471	arg1	NaCMC					1467:1471	NaCMC	1467:1471	NaCMC (0.1 g)	1467:1479	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	10	62	theme	NaCMC	1467:1471	arg1	0.01 g					1445:1450	0.01 g	1445:1450	0.01 g	1445:1450	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	10	62	theme	NaCMC	1467:1471	arg1	amount					1457:1462	amount	1457:1462	amount of NaCMC (0.1 g)	1457:1479	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	10	62	theme	NaCMC	1467:1471	arg1	MgO					1440:1442	MgO	1440:1442	MgO (0.01 g)	1440:1451	With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).
30184743	5	63	theme	appropriate	717:727	arg1	nanoparticle					729:740	an appropriate nanoparticle	714:740	an appropriate nanoparticle to be used in the nano composites	714:774	Thus, MgO is an appropriate nanoparticle to be used in the nano composites.
30184743	5	63	theme	appropriate	717:727	arg1	MgO					707:709	MgO	707:709	MgO	707:709	Thus, MgO is an appropriate nanoparticle to be used in the nano composites.
31404917	4	0	theme	cell-laden	627:636	arg1	bioink					638:643	a cell-laden bioink	625:643	a cell-laden bioink to build 3D bioprinted in vitro breast tumor models	625:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	4	0	theme	cell-laden	627:636	arg1	gels					600:603	The composite gels	586:603	The composite gels	586:603	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	6	1	theme	cancer	894:899	arg1	cells					901:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	2	2	theme	bioink	384:389	arg1	properties					366:375	the material properties	353:375	the material properties of the bioink such that it achieves both bioprintability and biomimicry	353:447	Tailoring the material properties of the bioink such that it achieves both bioprintability and biomimicry remains a key challenge.
31404917	6	3	theme	multicellular	988:1000	arg1	MCTS					1019:1022	MCTS	1019:1022	MCTS	1019:1022	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	6	3	theme	multicellular	988:1000	arg1	spheroids					1008:1016	multicellular tumor spheroids	988:1016	multicellular tumor spheroids (MCTS)	988:1023	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	5	4	theme	mechanical	710:719	arg1	characteristics					721:735	The initial mechanical characteristics	698:735	The initial mechanical characteristics of each composite hydrogel	698:762	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	6	5	theme	MDA-MB-231	876:885	arg1	cells					901:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	6	6	theme	self-assembly	969:981	arg1	frequency					956:964	frequency	956:964	frequency	956:964	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	6	6	theme	self-assembly	969:981	arg1	rates					946:950	rates	946:950	rates	946:950	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	1	7	theme	broad	215:219	arg1	spectrum					221:228	a broad spectrum	213:228	a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions	213:340	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	4	8	theme	breast	677:682	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	0	9	theme	growth	142:147	arg1	kinetics					149:156	tumor spheroid growth kinetics	127:156	tumor spheroid growth kinetics	127:156	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	1	10	dep	in	276:277	arg1	vitro					279:283	vitro	279:283	vitro	279:283	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	7	11	theme	MCTS	1266:1269	arg1	development					1245:1255	the development	1241:1255	the development of large MCTS	1241:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	4	12	dep	in	668:669	arg1	vitro					671:675	vitro	671:675	vitro	671:675	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	7	13	with	stiffer	1375:1381	arg1	moieties					1409:1416	fewer cell-adhesion moieties	1389:1416	fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS	1389:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	14	contain	contain	1182:1188	arg1	gels					1146:1149	gels	1146:1149	gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS	1146:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	14	contain	contain	1182:1188	arg2	number					1200:1205	a greater number	1190:1205	a greater number of cell-adhesion moieties driving the development of large MCTS	1190:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	5	15	theme	composite	745:753	arg1	hydrogel					755:762	each composite hydrogel	740:762	each composite hydrogel	740:762	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	1	16	theme	Tunable	159:165	arg1	materials					179:187	Tunable bioprinting materials	159:187	Tunable bioprinting materials	159:187	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	8	17	theme	mechanical	1595:1604	arg1	properties					1622:1631	both the mechanical and biochemical properties	1586:1631	properties	1622:1631	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	5	18	theme	spheroid	816:823	arg1	morphology					825:834	cell spheroid morphology	811:834	cell spheroid morphology	811:834	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	7	19	theme	alginate	1072:1079	arg1	concentrations					1081:1094	alginate concentrations	1072:1094	alginate concentrations	1072:1094	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	8	20	theme	biochemical	1610:1620	arg1	properties					1622:1631	both the mechanical and biochemical properties	1586:1631	properties	1622:1631	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	0	21	theme	adhesive	95:102	arg1	properties					104:113	tunable mechanical and cell adhesive properties	67:113	tunable mechanical and cell adhesive properties	67:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	7	22	theme	cell-adhesion	1210:1222	arg1	moieties					1224:1231	cell-adhesion moieties	1210:1231	cell-adhesion moieties driving the development of large MCTS	1210:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	5	23	theme	proliferation	787:799	arg1	rates					801:805	cell proliferation rates	782:805	cell proliferation rates	782:805	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	8	24	theme	native	1640:1645	arg1	stroma					1653:1658	the native tumor stroma	1636:1658	the native tumor stroma	1636:1658	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	0	25	theme	tumor	127:131	arg1	kinetics					149:156	tumor spheroid growth kinetics	127:156	tumor spheroid growth kinetics	127:156	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	8	26	theme	stroma	1653:1658	arg1	properties					1622:1631	both the mechanical and biochemical properties	1586:1631	properties	1622:1631	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	5	27	theme	cell	811:814	arg1	morphology					825:834	cell spheroid morphology	811:834	cell spheroid morphology	811:834	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	8	28	theme	composite	1493:1501	arg1	hydrogels					1503:1511	These composite hydrogels	1487:1511	These composite hydrogels	1487:1511	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	2	29	theme	key	459:461	arg1	challenge					463:471	a key challenge	457:471	a key challenge	457:471	Tailoring the material properties of the bioink such that it achieves both bioprintability and biomimicry remains a key challenge.
31404917	0	30	theme	alginate/gelatin	25:40	arg1	hydrogels					52:60	bioprintable alginate/gelatin composite hydrogels	12:60	bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties	12:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	1	31	theme	in	323:324	arg1	conditions					331:340	in vivo conditions	323:340	in vivo conditions	323:340	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	7	32	theme	composite	1433:1441	arg1	gels					1443:1446	the composite gels	1429:1446	the composite gels yielding smaller and less viable MCTS	1429:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	5	33	theme	long	851:854	arg1	conditions					864:873	month long culture conditions	845:873	month long culture conditions	845:873	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	7	34	theme	large	1260:1264	arg1	MCTS					1266:1269	large MCTS	1260:1269	large MCTS	1260:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	35	from	present	1418:1424	arg1	gels					1443:1446	the composite gels	1429:1446	the composite gels yielding smaller and less viable MCTS	1429:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	36	attach	present	1418:1424	arg2	moieties					1409:1416	fewer cell-adhesion moieties	1389:1416	fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS	1389:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	36	attach	present	1418:1424	arg1	gels					1443:1446	the composite gels	1429:1446	the composite gels yielding smaller and less viable MCTS	1429:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	3	37	theme	hydrogels	529:537	arg1	development					493:503	the development	489:503	the development of engineered composite hydrogels consisting of gelatin and alginate components	489:583	Here we report the development of engineered composite hydrogels consisting of gelatin and alginate components.
31404917	0	38	theme	tunable	67:73	arg1	properties					104:113	tunable mechanical and cell adhesive properties	67:113	tunable mechanical and cell adhesive properties	67:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	6	39	dep	rates	946:950	arg1	the					942:944	the	942:944	the	942:944	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	3	40	theme	engineered	508:517	arg1	hydrogels					529:537	engineered composite hydrogels	508:537	engineered composite hydrogels consisting of gelatin and alginate components	508:583	Here we report the development of engineered composite hydrogels consisting of gelatin and alginate components.
31404917	1	41	theme	3D	257:258	arg1	models					260:265	3D models	257:265	3D models enabling in vitro studies that more accurately resemble in vivo conditions	257:340	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	7	42	from	gels	1443:1446	arg1	present					1418:1424	present	1418:1424	present	1418:1424	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	43	theme	fewer	1389:1393	arg1	moieties					1409:1416	fewer cell-adhesion moieties	1389:1416	fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS	1389:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	4	44	theme	build	648:652	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	8	45	theme	in	1558:1559	arg1	systems					1567:1573	tunable in vitro systems	1550:1573	tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma	1550:1658	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	5	46	theme	culture	856:862	arg1	conditions					864:873	month long culture conditions	845:873	month long culture conditions	845:873	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	4	47	theme	bioprinted	657:666	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	7	48	theme	moieties	1224:1231	arg1	number					1200:1205	a greater number	1190:1205	a greater number of cell-adhesion moieties driving the development of large MCTS	1190:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	0	49	with	hydrogels	52:60	arg1	properties					104:113	tunable mechanical and cell adhesive properties	67:113	tunable mechanical and cell adhesive properties	67:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	6	50	theme	tumor	1002:1006	arg1	MCTS					1019:1022	MCTS	1019:1022	MCTS	1019:1022	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	6	50	theme	tumor	1002:1006	arg1	spheroids					1008:1016	multicellular tumor spheroids	988:1016	multicellular tumor spheroids (MCTS)	988:1023	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	5	51	theme	initial	702:708	arg1	characteristics					721:735	The initial mechanical characteristics	698:735	The initial mechanical characteristics of each composite hydrogel	698:762	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	6	52	theme	breast	887:892	arg1	cells					901:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells	876:905	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	8	53	dep	properties	1622:1631	arg1	both					1586:1589	both	1586:1589	both	1586:1589	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	7	54	theme	greater	1192:1198	arg1	number					1200:1205	a greater number	1190:1205	a greater number of cell-adhesion moieties driving the development of large MCTS	1190:1269	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	5	55	theme	hydrogel	755:762	arg1	characteristics					721:735	The initial mechanical characteristics	698:735	The initial mechanical characteristics of each composite hydrogel	698:762	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	8	56	theme	systems	1567:1573	arg1	biofabrication					1532:1545	the biofabrication	1528:1545	the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma	1528:1658	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	1	57	theme	physiological	233:245	arg1	spectrum					221:228	a broad spectrum	213:228	a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions	213:340	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	4	58	theme	tumor	684:688	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	6	59	from	formulation-dependency	916:937	arg1	frequency					956:964	frequency	956:964	frequency	956:964	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	6	59	from	formulation-dependency	916:937	arg1	rates					946:950	rates	946:950	rates	946:950	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	8	60	dep	in	1558:1559	arg1	vitro					1561:1565	vitro	1561:1565	vitro	1561:1565	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	7	61	dep	compositions	1035:1046	arg1	comprised					1048:1056	comprised	1048:1056	comprised of decreasing alginate concentrations	1048:1094	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	61	dep	compositions	1035:1046	arg1	increasing					1101:1110	increasing	1101:1110	increasing gelatin concentrations	1101:1133	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	62	theme	gelatin	1112:1118	arg1	concentrations					1120:1133	gelatin concentrations	1112:1133	gelatin concentrations	1112:1133	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	7	63	theme	gelatin	1335:1341	arg1	concentrations					1343:1356	gelatin concentrations	1335:1356	gelatin concentrations	1335:1356	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	1	64	theme	bioprinting	167:177	arg1	materials					179:187	Tunable bioprinting materials	159:187	Tunable bioprinting materials	159:187	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	3	65	theme	composite	519:527	arg1	hydrogels					529:537	engineered composite hydrogels	508:537	engineered composite hydrogels consisting of gelatin and alginate components	508:583	Here we report the development of engineered composite hydrogels consisting of gelatin and alginate components.
31404917	1	66	dep	in	323:324	arg1	vivo					326:329	vivo	326:329	vivo	326:329	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
31404917	7	67	theme	viable	1474:1479	arg1	MCTS					1481:1484	smaller and less viable MCTS	1457:1484	smaller and less viable MCTS	1457:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	5	68	theme	cell	782:785	arg1	rates					801:805	cell proliferation rates	782:805	cell proliferation rates	782:805	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	8	69	theme	tumor	1647:1651	arg1	stroma					1653:1658	the native tumor stroma	1636:1658	the native tumor stroma	1636:1658	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	2	70	theme	material	357:364	arg1	properties					366:375	the material properties	353:375	the material properties of the bioink such that it achieves both bioprintability and biomimicry	353:447	Tailoring the material properties of the bioink such that it achieves both bioprintability and biomimicry remains a key challenge.
31404917	0	71	theme	spheroid	133:140	arg1	kinetics					149:156	tumor spheroid growth kinetics	127:156	tumor spheroid growth kinetics	127:156	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	7	72	theme	smaller	1457:1463	arg1	MCTS					1481:1484	smaller and less viable MCTS	1457:1484	smaller and less viable MCTS	1457:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	0	73	theme	bioprintable	12:23	arg1	hydrogels					52:60	bioprintable alginate/gelatin composite hydrogels	12:60	bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties	12:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	7	74	theme	Hydrogel	1026:1033	arg1	compositions					1035:1046	Hydrogel compositions	1026:1046	Hydrogel compositions	1026:1046	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	0	75	theme	composite	42:50	arg1	hydrogels					52:60	bioprintable alginate/gelatin composite hydrogels	12:60	bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties	12:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	5	76	theme	month	845:849	arg1	conditions					864:873	month long culture conditions	845:873	month long culture conditions	845:873	The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions.
31404917	7	77	theme	present	1418:1424	arg1	moieties					1409:1416	fewer cell-adhesion moieties	1389:1416	fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS	1389:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	0	78	theme	mechanical	75:84	arg1	properties					104:113	tunable mechanical and cell adhesive properties	67:113	tunable mechanical and cell adhesive properties	67:113	Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.
31404917	7	79	theme	cell-adhesion	1395:1407	arg1	moieties					1409:1416	fewer cell-adhesion moieties	1389:1416	fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS	1389:1484	Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS.
31404917	4	80	theme	composite	590:598	arg1	bioink					638:643	a cell-laden bioink	625:643	a cell-laden bioink to build 3D bioprinted in vitro breast tumor models	625:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	4	80	theme	composite	590:598	arg1	gels					600:603	The composite gels	586:603	The composite gels	586:603	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	6	81	theme	gel	912:914	arg1	formulation-dependency					916:937	gel formulation-dependency	912:937	gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS)	912:1023	MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS).
31404917	4	82	theme	3D	654:655	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	8	83	used	used	1520:1523	arg2	hydrogels					1503:1511	These composite hydrogels	1487:1511	These composite hydrogels	1487:1511	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	4	84	theme	in	668:669	arg1	models					690:695	build 3D bioprinted in vitro breast tumor models	648:695	build 3D bioprinted in vitro breast tumor models	648:695	The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models.
31404917	8	85	theme	tunable	1550:1556	arg1	systems					1567:1573	tunable in vitro systems	1550:1573	tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma	1550:1658	These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma.
31404917	3	86	theme	alginate	565:572	arg1	components					574:583	alginate components	565:583	alginate components	565:583	Here we report the development of engineered composite hydrogels consisting of gelatin and alginate components.
31404917	1	87	theme	in	276:277	arg1	studies					285:291	in vitro studies	276:291	in vitro studies that more accurately resemble in vivo conditions	276:340	Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions.
29476992	2	0	dep	-trifluoroacetamide	489:507	arg1	MTSTFA					510:515	MTSTFA	510:515	MTSTFA	510:515	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	1	1	theme	accurate	256:263	arg1	study					281:285	accurate pharmacological study	256:285	accurate pharmacological study	256:285	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	1	2	theme	biological	173:182	arg1	activity					184:191	chondroitin sulfate (CS) biological activity	148:191	chondroitin sulfate (CS) biological activity	148:191	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	1	3	theme	pharmacological	265:279	arg1	study					281:285	accurate pharmacological study	256:285	accurate pharmacological study	256:285	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	2	4	attach	derived	373:379	arg2	CS					370:371	regioselective 4-O/6-O-desulfated CS	336:371	regioselective 4-O/6-O-desulfated CS derived from porcine	336:392	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	2	4	attach	derived	373:379	arg1	porcine					386:392	porcine	386:392	porcine	386:392	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	2	5	theme	CS	370:371	arg1	preparation					321:331	the preparation	317:331	the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine	317:392	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	5	6	theme	4-O/6-O-desulfated	920:937	arg1	CS					939:940	regioselective 4-O/6-O-desulfated CS	905:940	regioselective 4-O/6-O-desulfated CS	905:940	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	5	7	theme	regioselective	905:918	arg1	CS					939:940	regioselective 4-O/6-O-desulfated CS	905:940	regioselective 4-O/6-O-desulfated CS	905:940	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	2	8	theme	DMSO-MeOH	436:444	arg1	method					447:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	2	9	theme	dimethyl	407:414	arg1	method					447:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	3	10	theme	C6	700:701	arg1	position					703:710	C6 position	700:710	C6 position (6-O-S) of GalNAc	700:728	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	10	theme	C6	700:701	arg1	6-O-S					713:717	6-O-S	713:717	6-O-S	713:717	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	2	11	theme	-trifluoroacetamide	489:507	arg1	method					518:523	an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method	458:523	an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method	458:523	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	1	12	theme	sulfation	106:114	arg1	pattern					116:122	The sulfation pattern	102:122	The sulfation pattern	102:122	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	3	13	theme	C4	578:579	arg1	4-O-S					591:595	4-O-S	591:595	4-O-S	591:595	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	13	theme	C4	578:579	arg1	position					581:588	C4 position	578:588	C4 position (4-O-S) of N-acetylgalactosamine (GalNAc)	578:630	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	4	14	theme	disaccharide	850:861	arg1	analysis					875:882	disaccharide composition analysis	850:882	disaccharide composition analysis	850:882	Structures of desulfated CS were characterized by means of FT-IR, NMR and disaccharide composition analysis.
29476992	3	15	from	position	703:710	arg1	sulfate					689:695	the sulfate	685:695	the sulfate at C6 position (6-O-S) of GalNAc	685:728	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	16	theme	GalNAc	723:728	arg1	position					703:710	C6 position	700:710	C6 position (6-O-S) of GalNAc	700:728	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	16	theme	GalNAc	723:728	arg1	6-O-S					713:717	6-O-S	713:717	6-O-S	713:717	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	17	theme	DMSO-MeOH	663:671	arg1	method					673:678	the DMSO-MeOH method	659:678	the DMSO-MeOH method	659:678	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	5	18	theme	CS	939:940	arg1	preparations					889:900	The preparations	885:900	The preparations of regioselective 4-O/6-O-desulfated CS	885:940	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	5	18	theme	CS	939:940	arg1	powerful					946:953	powerful	946:953	powerful	946:953	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	4	19	theme	CS	801:802	arg1	Structures					776:785	Structures	776:785	Structures of desulfated CS	776:802	Structures of desulfated CS were characterized by means of FT-IR, NMR and disaccharide composition analysis.
29476992	0	20	theme	structural	16:25	arg1	characterization					27:42	structural characterization	16:42	structural characterization	16:42	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	1	21	theme	crucial	132:138	arg1	role					140:143	a crucial role	130:143	a crucial role	130:143	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	1	22	theme	CS	213:214	arg1	preparation					198:208	preparation	198:208	preparation of CS with defined structure	198:237	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	0	23	theme	regioselective	47:60	arg1	sulfate					93:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	4	24	theme	desulfated	790:799	arg1	CS					801:802	desulfated CS	790:802	desulfated CS	790:802	Structures of desulfated CS were characterized by means of FT-IR, NMR and disaccharide composition analysis.
29476992	1	25	theme	defined	221:227	arg1	structure					229:237	defined structure	221:237	defined structure	221:237	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	0	26	theme	chondroitin	81:91	arg1	sulfate					93:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	4	27	theme	composition	863:873	arg1	analysis					875:882	disaccharide composition analysis	850:882	disaccharide composition analysis	850:882	Structures of desulfated CS were characterized by means of FT-IR, NMR and disaccharide composition analysis.
29476992	2	28	theme	4-O/6-O-desulfated	351:368	arg1	CS					370:371	regioselective 4-O/6-O-desulfated CS	336:371	regioselective 4-O/6-O-desulfated CS derived from porcine	336:392	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	0	29	theme	4-O/6-O-desulfated	62:79	arg1	sulfate					93:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	regioselective 4-O/6-O-desulfated chondroitin sulfate	47:99	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	1	30	with	preparation	198:208	arg1	structure					229:237	defined structure	221:237	defined structure	221:237	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	2	31	dep	method	447:452	arg1	CS					525:526	CS	525:526	CS	525:526	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	2	32	theme	regioselective	336:349	arg1	CS					370:371	regioselective 4-O/6-O-desulfated CS	336:371	regioselective 4-O/6-O-desulfated CS derived from porcine	336:392	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	5	33	theme	structure-activity	972:989	arg1	relationship					991:1002	structure-activity relationship	972:1002	structure-activity relationship of CS	972:1008	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	1	34	theme	chondroitin	148:158	arg1	CS					169:170	CS	169:170	CS	169:170	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	1	34	theme	chondroitin	148:158	arg1	sulfate					160:166	chondroitin sulfate	148:166	chondroitin sulfate (CS) biological activity	148:191	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	5	35	theme	relationship	991:1002	arg1	study					963:967	the study	959:967	the study of structure-activity relationship of CS	959:1008	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
29476992	1	36	theme	sulfate	160:166	arg1	activity					184:191	chondroitin sulfate (CS) biological activity	148:191	chondroitin sulfate (CS) biological activity	148:191	The sulfation pattern plays a crucial role in chondroitin sulfate (CS) biological activity, and preparation of CS with defined structure is essential for accurate pharmacological study.
29476992	2	37	theme	sulfoxide-methanol	416:433	arg1	method					447:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	a dimethyl sulfoxide-methanol (DMSO-MeOH) method	405:452	In this study, we focused on the preparation of regioselective 4-O/6-O-desulfated CS derived from porcine, employing a dimethyl sulfoxide-methanol (DMSO-MeOH) method and an N-methyl-N-(trimethylsilyl) -trifluoroacetamide (MTSTFA) method CS, respectively.
29476992	3	38	theme	N-acetylgalactosamine	601:621	arg1	4-O-S					591:595	4-O-S	591:595	4-O-S	591:595	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	38	theme	N-acetylgalactosamine	601:621	arg1	position					581:588	C4 position	578:588	C4 position (4-O-S) of N-acetylgalactosamine (GalNAc)	578:630	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	39	from	position	581:588	arg1	sulfate					567:573	the sulfate	563:573	the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc)	563:630	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	3	40	theme	MTSTFA	761:766	arg1	method					768:773	the MTSTFA method	757:773	the MTSTFA method	757:773	Results showed that the sulfate at C4 position (4-O-S) of N-acetylgalactosamine (GalNAc) was selectively removed by the DMSO-MeOH method, and the sulfate at C6 position (6-O-S) of GalNAc was selectively removed by the MTSTFA method.
29476992	0	41	theme	sulfate	93:99	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	0	41	theme	sulfate	93:99	arg1	characterization					27:42	structural characterization	16:42	structural characterization	16:42	Preparation and structural characterization of regioselective 4-O/6-O-desulfated chondroitin sulfate.
29476992	5	42	theme	CS	1007:1008	arg1	relationship					991:1002	structure-activity relationship	972:1002	structure-activity relationship of CS	972:1008	The preparations of regioselective 4-O/6-O-desulfated CS are powerful for the study of structure-activity relationship of CS.
31724692	5	0	theme	actuator	1026:1033	arg1	modelling					1009:1017	a fine mechanic modelling	993:1017	a fine mechanic modelling of the actuator	993:1033	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	5	0	theme	actuator	1026:1033	arg1	profiles					980:987	relative humidity-curvature profiles	952:987	relative humidity-curvature profiles	952:987	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	5	0	theme	actuator	1026:1033	arg1	power-curvature					935:949	The well-established light power-curvature	908:949	The well-established light power-curvature	908:949	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	6	1	theme	low	1223:1225	arg1	weight					1232:1237	a low body weight	1221:1237	a low body weight	1221:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	2	2	theme	single	330:335	arg1	stimulus					337:344	a single stimulus	328:344	a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications	328:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	1	3	theme	multiple	234:241	arg1	forms					243:247	multiple forms	234:247	multiple forms of movements	234:260	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	4	4	theme	actuation	823:831	arg1	curvature					833:841	an ultra-large actuation curvature	808:841	an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation)	808:905	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	3	5	theme	bidirectional	466:478	arg1	material					490:497	bidirectional actuating material	466:497	bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films	466:557	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	0	6	theme	chitosan	98:105	arg1	film					107:110	a chitosan film	96:110	a chitosan film	96:110	An ultra-large deformation bidirectional actuator based on a carbon nanotube/PDMS composite and a chitosan film.
31724692	5	7	theme	relative	952:959	arg1	profiles					980:987	relative humidity-curvature profiles	952:987	relative humidity-curvature profiles	952:987	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	3	8	theme	actuating	480:488	arg1	material					490:497	bidirectional actuating material	466:497	bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films	466:557	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	3	9	theme	carbon	508:513	arg1	composites					529:538	carbon nanotube/PDMS composites	508:538	carbon nanotube/PDMS composites	508:538	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	3	10	theme	chitosan	544:551	arg1	films					553:557	chitosan films	544:557	chitosan films	544:557	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	1	11	dep	convert	137:143	arg1	etc.					193:196	etc.	193:196	etc.	193:196	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	1	11	dep	convert	137:143	arg1	electricity					180:190	electricity	180:190	electricity	180:190	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	1	11	dep	convert	137:143	arg1	light					173:177	light	173:177	light	173:177	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	1	11	dep	convert	137:143	arg1	humidity					163:170	humidity	163:170	humidity	163:170	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	3	12	theme	nanotube/PDMS	515:527	arg1	composites					529:538	carbon nanotube/PDMS composites	508:538	carbon nanotube/PDMS composites	508:538	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	1	13	theme	movements	252:260	arg1	forms					243:247	multiple forms	234:247	multiple forms of movements	234:260	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	3	14	theme	new	454:456	arg1	type					458:461	a new type	452:461	a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films	452:557	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	4	15	theme	light	711:715	arg1	absorption					717:726	strong near-infrared light absorption	690:726	strong near-infrared light absorption by carbon nanotube layers	690:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	5	16	theme	light	929:933	arg1	power-curvature					935:949	The well-established light power-curvature	908:949	The well-established light power-curvature	908:949	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	4	17	theme	ultra-large	632:642	arg1	capacity					650:657	the ultra-large water capacity	628:657	the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers	628:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	0	18	theme	ultra-large	3:13	arg1	actuator					41:48	An ultra-large deformation bidirectional actuator	0:48	An ultra-large deformation bidirectional actuator based on a carbon	0:66	An ultra-large deformation bidirectional actuator based on a carbon nanotube/PDMS composite and a chitosan film.
31724692	6	19	theme	cargo-moving	1118:1129	arg1	device					1131:1136	a cargo-moving device	1116:1136	a cargo-moving device	1116:1136	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	4	20	theme	ultra-large	811:821	arg1	curvature					833:841	an ultra-large actuation curvature	808:841	an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation)	808:905	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	6	21	with	actuator	1207:1214	arg1	weight					1232:1237	a low body weight	1221:1237	a low body weight	1221:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	2	22	theme	actuating	294:302	arg1	materials					304:312	current actuating materials	286:312	current actuating materials	286:312	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	2	23	theme	small	353:357	arg1	degree					359:364	a small degree	351:364	a small degree of actuation and rough control which is unfavorable for practical applications	351:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	2	23	theme	small	353:357	arg1	unfavorable					406:416	unfavorable	406:416	unfavorable	406:416	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	6	24	theme	mechanical	1174:1183	arg1	functionality					1185:1197	a robust mechanical functionality	1165:1197	a robust mechanical functionality of this actuator with a low body weight	1165:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	4	25	from	cm-1	849:852	arg1	actuation					866:874	humidity actuation	857:874	humidity actuation	857:874	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	2	26	theme	current	286:292	arg1	materials					304:312	current actuating materials	286:312	current actuating materials	286:312	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	0	27	theme	bidirectional	27:39	arg1	actuator					41:48	An ultra-large deformation bidirectional actuator	0:48	An ultra-large deformation bidirectional actuator based on a carbon	0:66	An ultra-large deformation bidirectional actuator based on a carbon nanotube/PDMS composite and a chitosan film.
31724692	4	28	theme	near-infrared	697:709	arg1	absorption					717:726	strong near-infrared light absorption	690:726	strong near-infrared light absorption by carbon nanotube layers	690:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	2	29	theme	control	389:395	arg1	degree					359:364	a small degree	351:364	a small degree of actuation and rough control which is unfavorable for practical applications	351:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	2	29	theme	control	389:395	arg1	unfavorable					406:416	unfavorable	406:416	unfavorable	406:416	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	0	30	theme	deformation	15:25	arg1	actuator					41:48	An ultra-large deformation bidirectional actuator	0:48	An ultra-large deformation bidirectional actuator based on a carbon	0:66	An ultra-large deformation bidirectional actuator based on a carbon nanotube/PDMS composite and a chitosan film.
31724692	5	31	theme	fine	995:998	arg1	modelling					1009:1017	a fine mechanic modelling	993:1017	a fine mechanic modelling of the actuator	993:1033	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	2	32	theme	rough	383:387	arg1	control					389:395	rough control	383:395	rough control	383:395	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	6	33	theme	actuator	1207:1214	arg1	functionality					1185:1197	a robust mechanical functionality	1165:1197	a robust mechanical functionality of this actuator with a low body weight	1165:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	4	34	from	cm-1	882:885	arg1	actuation					896:904	light actuation	890:904	light actuation	890:904	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	6	35	theme	body	1227:1230	arg1	weight					1232:1237	a low body weight	1221:1237	a low body weight	1221:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	4	36	theme	carbon	731:736	arg1	layers					747:752	carbon nanotube layers	731:752	carbon nanotube layers	731:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	2	37	theme	actuation	369:377	arg1	degree					359:364	a small degree	351:364	a small degree of actuation and rough control which is unfavorable for practical applications	351:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	2	37	theme	actuation	369:377	arg1	unfavorable					406:416	unfavorable	406:416	unfavorable	406:416	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	4	38	dep	curvature	833:841	arg1	cm-1					849:852	3.91 cm-1	844:852	3.91 cm-1 in humidity actuation	844:874	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	4	38	dep	curvature	833:841	arg1	cm-1					882:885	3.84 cm-1	877:885	3.84 cm-1 in light actuation	877:904	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	5	39	theme	well-established	912:927	arg1	power-curvature					935:949	The well-established light power-curvature	908:949	The well-established light power-curvature	908:949	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	1	40	theme	Actuating	113:121	arg1	materials					123:131	Actuating materials	113:131	Actuating materials	113:131	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	2	41	theme	practical	422:430	arg1	applications					432:443	practical applications	422:443	practical applications	422:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	5	42	theme	mechanic	1000:1007	arg1	modelling					1009:1017	a fine mechanic modelling	993:1017	a fine mechanic modelling of the actuator	993:1033	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	4	43	theme	chitosan	670:677	arg1	chains					679:684	chitosan chains	670:684	chitosan chains	670:684	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	6	44	theme	robust	1167:1172	arg1	functionality					1185:1197	a robust mechanical functionality	1165:1197	a robust mechanical functionality of this actuator with a low body weight	1165:1237	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	4	45	theme	water	644:648	arg1	capacity					650:657	the ultra-large water capacity	628:657	the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers	628:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	4	46	theme	strong	690:695	arg1	absorption					717:726	strong near-infrared light absorption	690:726	strong near-infrared light absorption by carbon nanotube layers	690:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	1	47	theme	mechanical	204:213	arg1	energy					215:220	mechanical energy	204:220	mechanical energy	204:220	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	4	48	theme	humidity	857:864	arg1	actuation					866:874	humidity actuation	857:874	humidity actuation	857:874	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	4	49	theme	nanotube	738:745	arg1	layers					747:752	carbon nanotube layers	731:752	carbon nanotube layers	731:752	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	2	50	theme	materials	304:312	arg1	majority					274:281	a majority	272:281	a majority of current actuating materials	272:312	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	6	51	theme	lab	1097:1099	arg1	application					1101:1111	A lab application	1095:1111	A lab application as a cargo-moving device	1095:1136	A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.
31724692	2	52	with	stimulus	337:344	arg1	degree					359:364	a small degree	351:364	a small degree of actuation and rough control which is unfavorable for practical applications	351:443	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	2	52	with	stimulus	337:344	arg1	unfavorable					406:416	unfavorable	406:416	unfavorable	406:416	However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications.
31724692	5	53	theme	humidity-curvature	961:978	arg1	profiles					980:987	relative humidity-curvature profiles	952:987	relative humidity-curvature profiles	952:987	The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending.
31724692	1	54	theme	external	145:152	arg1	stimuli					154:160	external stimuli	145:160	external stimuli	145:160	Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements.
31724692	4	55	theme	mechanical	593:602	arg1	support					604:610	the robust mechanical support	582:610	the robust mechanical support by PDMS	582:618	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	4	56	theme	robust	586:591	arg1	support					604:610	the robust mechanical support	582:610	the robust mechanical support by PDMS	582:618	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
31724692	3	57	theme	material	490:497	arg1	type					458:461	a new type	452:461	a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films	452:557	Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed.
31724692	4	58	theme	light	890:894	arg1	actuation					896:904	light actuation	890:904	light actuation	890:904	Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation).
30395875	6	0	theme	No	996:997	arg1	formation					1011:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	10	1	theme	caries	1396:1401	arg1	level					1412:1416	the caries activity level	1392:1416	the caries activity level of the patient	1392:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	4	2	theme	mineral	796:802	arg1	loss					804:807	mineral loss	796:807	mineral loss	796:807	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	10	3	from	model	1541:1545	arg1	related					1477:1483	related	1477:1483	related	1477:1483	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	3	4	theme	sucrose	651:657	arg1	x					679:679	a 20% sucrose solution (10 min) 8 x	645:679	a 20% sucrose solution (10 min) 8 x per day	645:687	The splint was dipped in a 20% sucrose solution (10 min) 8 x per day.
30395875	7	5	theme	independent	1118:1128	arg1	progression					1106:1116	SC lesion depth progression	1090:1116	SC lesion depth progression independent of caries activity level of volunteers	1090:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	1	6	theme	activity	238:245	arg1	level					247:251	the caries activity level	227:251	the caries activity level from volunteers	227:267	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	3	7	theme	20	647:648	arg1	%					649:649	%	649:649	%	649:649	The splint was dipped in a 20% sucrose solution (10 min) 8 x per day.
30395875	10	8	from	related	1477:1483	arg1	model					1541:1545	this standardized in situ model	1515:1545	this standardized in situ model	1515:1545	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	11	9	theme	caries	1653:1658	arg1	lesion					1665:1670	secondary caries wall lesion	1643:1670	secondary caries wall lesion	1643:1670	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	10	10	theme	wall	1440:1443	arg1	progression					1452:1462	SC wall lesion progression	1437:1462	SC wall lesion progression	1437:1462	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	5	11	theme	caries	964:969	arg1	volunteers					919:928	the volunteers	915:928	the volunteers	915:928	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	11	theme	caries	964:969	arg1	levels					980:985	high, mid and low caries activity levels	946:985	high, mid and low caries activity levels	946:985	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	2	12	dep	90 μm	564:568	arg1	gap					614:616	intentional gap	602:616	intentional gap	602:616	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	12	dep	90 μm	564:568	arg1	procedure					583:591	no adhesive procedure	571:591	no adhesive procedure	571:591	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	6	13	theme	lesion	1004:1009	arg1	formation					1011:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	8	14	theme	substantial	1173:1183	arg1	lesions					1190:1196	No substantial wall lesions	1170:1196	No substantial wall lesions	1170:1196	No substantial wall lesions were found for two volunteers.
30395875	5	15	theme	wall	834:837	arg1	depth					846:850	Average wall lesion depth	826:850	Average wall lesion depth from each volunteer	826:870	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	11	16	theme	patient	1607:1613	arg1	size					1634:1637	the threshold gap size	1616:1637	the threshold gap size	1616:1637	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	11	16	theme	patient	1607:1613	arg1	level					1594:1598	caries activity level	1578:1598	caries activity level of the patient	1578:1613	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	1	17	theme	caries	231:236	arg1	level					247:251	the caries activity level	227:251	the caries activity level from volunteers	227:267	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	1	18	theme	gaps	133:136	arg1	influence					109:117	the influence	105:117	the influence of very small gaps in secondary caries (SC) development	105:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	1	19	dep	OBJECTIVES	70:79	arg1	linked					192:197	linked	192:197	additionally linked the threshold gap size with the caries activity level from volunteers	179:267	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	1	19	dep	OBJECTIVES	70:79	arg1	investigated					92:103	investigated	92:103	investigated the influence of very small gaps in secondary caries (SC) development	92:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	11	20	theme	gap	1630:1632	arg1	level					1594:1598	caries activity level	1578:1598	caries activity level of the patient	1578:1613	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	11	20	theme	gap	1630:1632	arg1	size					1634:1637	the threshold gap size	1616:1637	the threshold gap size	1616:1637	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	7	21	theme	lesion	1093:1098	arg1	progression					1106:1116	SC lesion depth progression	1090:1116	SC lesion depth progression independent of caries activity level of volunteers	1090:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	1	22	theme	secondary	141:149	arg1	development					163:173	secondary caries (SC) development	141:173	secondary caries (SC) development	141:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	2	23	theme	composite	504:512	arg1	resin					514:518	composite resin	504:518	composite resin	504:518	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	24	theme	intentional	602:612	arg1	gap					614:616	intentional gap	602:616	intentional gap	602:616	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	25	theme	different	388:396	arg1	interfaces					398:407	different interfaces	388:407	different interfaces	388:407	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	9	26	theme	activity	1296:1303	arg1	group					1305:1309	the high activity group	1287:1309	the high activity group	1287:1309	A trend for deeper lesion in larger gaps was observed for the high activity group.
30395875	0	27	theme	lesion	50:55	arg1	development					57:67	in situ secondary caries lesion development	25:67	in situ secondary caries lesion development	25:67	A threshold gap size for in situ secondary caries lesion development.
30395875	0	28	theme	gap	12:14	arg1	size					16:19	A threshold gap size	0:19	A threshold gap size for in situ secondary caries lesion development	0:67	A threshold gap size for in situ secondary caries lesion development.
30395875	2	29	theme	adhesive	453:460	arg1	procedure					462:470	complete adhesive procedure	444:470	complete adhesive procedure	444:470	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	10	30	theme	than	1355:1358	arg1	30 μm					1360:1364	wider than 30 μm	1349:1364	wider than 30 μm develop SC independent of the caries activity level of the patient	1349:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	4	31	theme	wavelength	727:736	arg1	T-WIM					768:772	T-WIM	768:772	T-WIM	768:772	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	4	31	theme	wavelength	727:736	arg1	microradiography					750:765	transversal wavelength independent microradiography	715:765	transversal wavelength independent microradiography (T-WIM)	715:773	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	10	32	theme	standardized	1520:1531	arg1	model					1541:1545	this standardized in situ model	1515:1545	this standardized in situ model	1515:1545	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	0	33	theme	caries	43:48	arg1	development					57:67	in situ secondary caries lesion development	25:67	in situ secondary caries lesion development	25:67	A threshold gap size for in situ secondary caries lesion development.
30395875	10	34	dep	CONCLUSION	1312:1321	arg1	seemed					1464:1469	seemed	1464:1469	seemed to be related to individual factors even in this standardized in situ model	1464:1545	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	5	35	theme	high	946:949	arg1	volunteers					919:928	the volunteers	915:928	the volunteers	915:928	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	35	theme	high	946:949	arg1	levels					980:985	high, mid and low caries activity levels	946:985	high, mid and low caries activity levels	946:985	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	11	36	theme	level	1594:1598	arg1	SIGNIFICANCE					1548:1559	SIGNIFICANCE	1548:1559	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion	1548:1670	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	9	37	theme	larger	1258:1263	arg1	gaps					1265:1268	larger gaps	1258:1268	larger gaps	1258:1268	A trend for deeper lesion in larger gaps was observed for the high activity group.
30395875	11	38	theme	caries	1578:1583	arg1	size					1634:1637	the threshold gap size	1616:1637	the threshold gap size	1616:1637	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	11	38	theme	caries	1578:1583	arg1	level					1594:1598	caries activity level	1578:1598	caries activity level of the patient	1578:1613	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	1	39	theme	gap	213:215	arg1	size					217:220	the threshold gap size	199:220	the threshold gap size	199:220	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	6	40	theme	wall	999:1002	arg1	formation					1011:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	10	41	theme	activity	1403:1410	arg1	level					1412:1416	the caries activity level	1392:1416	the caries activity level of the patient	1392:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	2	42	theme	modified	312:319	arg1	splint					330:335	a modified occlusal splint	310:335	a modified occlusal splint loaded with dentin-composite samples restored with different interfaces	310:407	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	4	43	theme	lesion	779:784	arg1	depth					786:790	lesion depth	779:790	lesion depth	779:790	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	6	44	theme	RESULTS	988:994	arg1	formation					1011:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	10	45	theme	SC	1437:1438	arg1	progression					1452:1462	SC wall lesion progression	1437:1462	SC wall lesion progression	1437:1462	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	9	46	theme	high	1291:1294	arg1	group					1305:1309	the high activity group	1287:1309	the high activity group	1287:1309	A trend for deeper lesion in larger gaps was observed for the high activity group.
30395875	10	47	theme	patient	1425:1431	arg1	level					1412:1416	the caries activity level	1392:1416	the caries activity level of the patient	1392:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	3	48	theme	solution	659:666	arg1	x					679:679	a 20% sucrose solution (10 min) 8 x	645:679	a 20% sucrose solution (10 min) 8 x per day	645:687	The splint was dipped in a 20% sucrose solution (10 min) 8 x per day.
30395875	1	49	from	volunteers	258:267	arg1	level					247:251	the caries activity level	227:251	the caries activity level from volunteers	227:267	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	1	50	theme	caries	151:156	arg1	development					163:173	secondary caries (SC) development	141:173	secondary caries (SC) development	141:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	2	51	theme	adhesive	574:581	arg1	procedure					583:591	no adhesive procedure	571:591	no adhesive procedure	571:591	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	3	52	theme	%	649:649	arg1	x					679:679	a 20% sucrose solution (10 min) 8 x	645:679	a 20% sucrose solution (10 min) 8 x per day	645:687	The splint was dipped in a 20% sucrose solution (10 min) 8 x per day.
30395875	1	53	theme	SC	159:160	arg1	development					163:173	secondary caries (SC) development	141:173	secondary caries (SC) development	141:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	6	54	theme	NB	1043:1044	arg1	groups					1046:1051	B and NB groups	1037:1051	B and NB groups	1037:1051	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	10	55	theme	lesion	1445:1450	arg1	progression					1452:1462	SC wall lesion progression	1437:1462	SC wall lesion progression	1437:1462	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	11	56	theme	wall	1660:1663	arg1	lesion					1665:1670	secondary caries wall lesion	1643:1670	secondary caries wall lesion	1643:1670	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	10	57	dep	in	1533:1534	arg1	situ					1536:1539	situ	1536:1539	situ	1536:1539	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	2	58	theme	adhesive	528:535	arg1	procedure					537:545	adhesive procedure	528:545	adhesive procedure	528:545	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	6	59	theme	B	1037:1037	arg1	groups					1046:1051	B and NB groups	1037:1051	B and NB groups	1037:1051	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	3	60	theme	10 min	669:674	arg1	x					679:679	a 20% sucrose solution (10 min) 8 x	645:679	a 20% sucrose solution (10 min) 8 x per day	645:687	The splint was dipped in a 20% sucrose solution (10 min) 8 x per day.
30395875	5	61	theme	activity	971:978	arg1	volunteers					919:928	the volunteers	915:928	the volunteers	915:928	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	61	theme	activity	971:978	arg1	levels					980:985	high, mid and low caries activity levels	946:985	high, mid and low caries activity levels	946:985	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	62	theme	Average	826:832	arg1	depth					846:850	Average wall lesion depth	826:850	Average wall lesion depth from each volunteer	826:870	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	6	63	located	observed	1025:1032	arg1	groups					1046:1051	B and NB groups	1037:1051	B and NB groups	1037:1051	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	6	63	located	observed	1025:1032	arg2	formation					1011:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation	988:1019	RESULTS No wall lesion formation was observed in B and NB groups.
30395875	2	64	dep	METHODS	270:276	arg1	no-bonded					474:482	no-bonded	474:482	no-bonded	474:482	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	64	dep	METHODS	270:276	arg1	bonded					410:415	bonded	410:415	bonded	410:415	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	64	dep	METHODS	270:276	arg1	30					553:554	30	553:554	30	553:554	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	5	65	theme	mid	952:954	arg1	volunteers					919:928	the volunteers	915:928	the volunteers	915:928	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	65	theme	mid	952:954	arg1	levels					980:985	high, mid and low caries activity levels	946:985	high, mid and low caries activity levels	946:985	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	66	theme	lesion	839:844	arg1	depth					846:850	Average wall lesion depth	826:850	Average wall lesion depth from each volunteer	826:870	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	8	67	theme	wall	1185:1188	arg1	lesions					1190:1196	No substantial wall lesions	1170:1196	No substantial wall lesions	1170:1196	No substantial wall lesions were found for two volunteers.
30395875	7	68	theme	depth	1100:1104	arg1	progression					1106:1116	SC lesion depth progression	1090:1116	SC lesion depth progression independent of caries activity level of volunteers	1090:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	5	69	theme	low	960:962	arg1	volunteers					919:928	the volunteers	915:928	the volunteers	915:928	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	5	69	theme	low	960:962	arg1	levels					980:985	high, mid and low caries activity levels	946:985	high, mid and low caries activity levels	946:985	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	1	70	theme	small	127:131	arg1	gaps					133:136	very small gaps	122:136	very small gaps	122:136	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	7	71	theme	SC	1090:1091	arg1	progression					1106:1116	SC lesion depth progression	1090:1116	SC lesion depth progression independent of caries activity level of volunteers	1090:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	9	72	from	lesion	1248:1253	arg1	gaps					1265:1268	larger gaps	1258:1268	larger gaps	1258:1268	A trend for deeper lesion in larger gaps was observed for the high activity group.
30395875	11	73	theme	threshold	1620:1628	arg1	level					1594:1598	caries activity level	1578:1598	caries activity level of the patient	1578:1613	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	11	73	theme	threshold	1620:1628	arg1	size					1634:1637	the threshold gap size	1616:1637	the threshold gap size	1616:1637	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	7	74	theme	intentional	1066:1076	arg1	gaps					1078:1081	intentional gaps	1066:1081	intentional gaps	1066:1081	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	2	75	dep	bonded	410:415	arg1	B = samples					418:428	B = samples	418:428	B = samples	418:428	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	2	76	theme	dentin-composite	349:364	arg1	samples					366:372	dentin-composite samples	349:372	dentin-composite samples restored with different interfaces	349:407	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	0	77	theme	threshold	2:10	arg1	size					16:19	A threshold gap size	0:19	A threshold gap size for in situ secondary caries lesion development	0:67	A threshold gap size for in situ secondary caries lesion development.
30395875	11	78	theme	secondary	1643:1651	arg1	lesion					1665:1670	secondary caries wall lesion	1643:1670	secondary caries wall lesion	1643:1670	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	10	79	theme	small	1328:1332	arg1	gaps					1334:1337	Very small gaps	1323:1337	Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression	1323:1462	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	10	80	theme	individual	1488:1497	arg1	factors					1499:1505	individual factors	1488:1505	individual factors	1488:1505	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	1	81	from	influence	109:117	arg1	development					163:173	secondary caries (SC) development	141:173	secondary caries (SC) development	141:173	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	2	82	dep	volunteers	294:303	arg1	wore					305:308	wore	305:308	wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces	305:407	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	10	83	theme	in	1533:1534	arg1	model					1541:1545	this standardized in situ model	1515:1545	this standardized in situ model	1515:1545	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	0	84	theme	in	25:26	arg1	development					57:67	in situ secondary caries lesion development	25:67	in situ secondary caries lesion development	25:67	A threshold gap size for in situ secondary caries lesion development.
30395875	2	85	theme	complete	444:451	arg1	procedure					462:470	complete adhesive procedure	444:470	complete adhesive procedure	444:470	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	10	86	theme	wider	1349:1353	arg1	30 μm					1360:1364	wider than 30 μm	1349:1364	wider than 30 μm develop SC independent of the caries activity level of the patient	1349:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	4	87	theme	transversal	715:725	arg1	T-WIM					768:772	T-WIM	768:772	T-WIM	768:772	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	4	87	theme	transversal	715:725	arg1	microradiography					750:765	transversal wavelength independent microradiography	715:765	transversal wavelength independent microradiography (T-WIM)	715:773	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	10	88	dep	30 μm	1360:1364	arg1	develop					1366:1372	develop	1366:1372	develop SC independent of the caries activity level of the patient	1366:1431	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	0	89	theme	secondary	33:41	arg1	development					57:67	in situ secondary caries lesion development	25:67	in situ secondary caries lesion development	25:67	A threshold gap size for in situ secondary caries lesion development.
30395875	9	90	theme	deeper	1241:1246	arg1	lesion					1248:1253	deeper lesion	1241:1253	deeper lesion in larger gaps	1241:1268	A trend for deeper lesion in larger gaps was observed for the high activity group.
30395875	0	91	dep	in	25:26	arg1	situ					28:31	situ	28:31	situ	28:31	A threshold gap size for in situ secondary caries lesion development.
30395875	7	92	theme	volunteers	1158:1167	arg1	level					1149:1153	caries activity level	1133:1153	caries activity level of volunteers	1133:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	11	93	theme	activity	1585:1592	arg1	size					1634:1637	the threshold gap size	1616:1637	the threshold gap size	1616:1637	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	11	93	theme	activity	1585:1592	arg1	level					1594:1598	caries activity level	1578:1598	caries activity level of the patient	1578:1613	SIGNIFICANCE Independently of caries activity level of the patient, the threshold gap size for secondary caries wall lesion seems to be 30 μm at most.
30395875	4	94	theme	independent	738:748	arg1	T-WIM					768:772	T-WIM	768:772	T-WIM	768:772	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	4	94	theme	independent	738:748	arg1	microradiography					750:765	transversal wavelength independent microradiography	715:765	transversal wavelength independent microradiography (T-WIM)	715:773	Samples were imaged with transversal wavelength independent microradiography (T-WIM) and lesion depth and mineral loss were calculated.
30395875	5	95	from	volunteer	862:870	arg1	depth					846:850	Average wall lesion depth	826:850	Average wall lesion depth from each volunteer	826:870	Average wall lesion depth from each volunteer was determined and according to the values the volunteers were grouped as high, mid and low caries activity levels.
30395875	2	96	dep	no-bonded	474:482	arg1	NB = restored					485:497	NB = restored	485:497	NB = restored with composite resin without adhesive procedure	485:545	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
30395875	7	97	theme	level	1149:1153	arg1	independent					1118:1128	independent	1118:1128	independent	1118:1128	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	7	98	theme	activity	1140:1147	arg1	level					1149:1153	caries activity level	1133:1153	caries activity level of volunteers	1133:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	1	99	theme	threshold	203:211	arg1	size					217:220	the threshold gap size	199:220	the threshold gap size	199:220	OBJECTIVES This study investigated the influence of very small gaps in secondary caries (SC) development and additionally linked the threshold gap size with the caries activity level from volunteers.
30395875	7	100	theme	caries	1133:1138	arg1	level					1149:1153	caries activity level	1133:1153	caries activity level of volunteers	1133:1167	In general, intentional gaps led to SC lesion depth progression independent of caries activity level of volunteers.
30395875	10	101	theme	level	1412:1416	arg1	independent					1377:1387	independent	1377:1387	independent	1377:1387	CONCLUSION Very small gaps around or wider than 30 μm develop SC independent of the caries activity level of the patient and SC wall lesion progression seemed to be related to individual factors even in this standardized in situ model.
30395875	2	102	theme	occlusal	321:328	arg1	splint					330:335	a modified occlusal splint	310:335	a modified occlusal splint loaded with dentin-composite samples restored with different interfaces	310:407	METHODS For 21 days, 15 volunteers wore a modified occlusal splint loaded with dentin-composite samples restored with different interfaces: bonded (B = samples restored with complete adhesive procedure), no-bonded (NB = restored with composite resin without adhesive procedure), and 30, 60 and 90 μm (no adhesive procedure and with intentional gap).
31041762	9	0	theme	pathogen	1689:1696	arg1	growth					1698:1703	pathogen growth	1689:1703	pathogen growth	1689:1703	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	8	1	theme	mechanical	1415:1424	arg1	properties					1426:1435	mechanical properties	1415:1435	mechanical properties of pectin	1415:1445	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	6	2	dep	Mohnen	1095:1100	arg1	Biol					1119:1122	Biol	1119:1122	Biol	1119:1122	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	1	3	theme	Plant	95:99	arg1	source					161:166	a source	159:166	a source of signaling molecules that can modulate plant immunity	159:222	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	1	3	theme	Plant	95:99	arg1	barrier					126:132	a barrier	124:132	a barrier to pathogen entry	124:150	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	1	3	theme	Plant	95:99	arg1	walls					106:110	Plant cell walls	95:110	Plant cell walls	95:110	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	2	4	theme	polymeric	260:268	arg1	hemicellulose					301:313	hemicellulose	301:313	hemicellulose	301:313	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	4	theme	polymeric	260:268	arg1	cellulose					278:286	cellulose	278:286	cellulose	278:286	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	4	theme	polymeric	260:268	arg1	sugars					270:275	three polymeric sugars	254:275	three polymeric sugars: cellulose, pectin, and hemicellulose	254:313	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	4	theme	polymeric	260:268	arg1	pectin					289:294	pectin	289:294	pectin	289:294	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	9	5	dep	Lionetti	1756:1763	arg1	Bethke					1810:1815	Bethke	1810:1815	Bethke	1810:1815	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	5	dep	Lionetti	1756:1763	arg1	Physiol					1781:1787	Physiol	1781:1787	Physiol	1781:1787	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	5	dep	Lionetti	1756:1763	arg1	al.					1768:1770	Lionetti et al., J Plant Physiol 169:1623-1630	1756:1801	al.	1768:1770	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	5	dep	Lionetti	1756:1763	arg1	Raiola					1854:1859	Raiola	1854:1859	Raiola	1854:1859	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	7	6	theme	methylesterification	1222:1241	arg1	amount					1188:1193	the amount	1184:1193	the amount	1184:1193	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	6	theme	methylesterification	1222:1241	arg1	patterns					1203:1210	the patterns	1199:1210	the patterns of pectin methylesterification in the wall	1199:1253	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	6	theme	methylesterification	1222:1241	arg1	methylesterification					1222:1241	pectin methylesterification	1215:1241	pectin methylesterification	1215:1241	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	10	7	theme	total	2169:2173	arg1	content					2182:2188	total pectin content	2169:2188	total pectin content	2169:2188	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	9	8	theme	Physiol	1727:1733	arg1	24:432-440					1896:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	4	9	theme	various	540:546	arg1	types					548:552	various types	540:552	various types of pectin	540:562	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	1	10	theme	molecules	181:189	arg1	source					161:166	a source	159:166	a source of signaling molecules that can modulate plant immunity	159:222	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	1	10	theme	molecules	181:189	arg1	walls					106:110	Plant cell walls	95:110	Plant cell walls	95:110	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	10	11	theme	pectin	2221:2226	arg1	composition					2228:2238	pectin composition	2221:2238	pectin composition	2221:2238	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	9	12	theme	Plant	1928:1932	arg1	Bethke					1706:1711	Bethke	1706:1711	Bethke	1706:1711	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	12	theme	Plant	1928:1932	arg1	Cell					1934:1937	Plant Cell 14:2095-2106	1928:1950	Plant Cell 14:2095-2106	1928:1950	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	13	dep	Bethke	1706:1711	arg1	al.					1716:1718	Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011	1706:1911	al.	1716:1718	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	13	dep	Bethke	1706:1711	arg1	Vogel					1914:1918	Vogel	1914:1918	Vogel	1914:1918	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	13	dep	Bethke	1706:1711	arg1	Wietholter					1999:2008	Wietholter	1999:2008	Wietholter	1999:2008	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	13	dep	Bethke	1706:1711	arg1	24:432-440					1896:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	13	dep	Bethke	1706:1711	arg1	Vogel					1959:1963	Vogel	1959:1963	Vogel	1959:1963	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	14	dep	Wietholter	1999:2008	arg1	al.					2013:2015	Wietholter et al.	1999:2015	Wietholter et al.	1999:2015	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	6	15	dep	Harholt	1046:1052	arg1	al.					1057:1059	Harholt et al., Plant Physiol 153:384-395	1046:1086	al.	1057:1059	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	6	15	dep	Harholt	1046:1052	arg1	Physiol					1068:1074	Physiol	1068:1074	Physiol	1068:1074	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	6	15	dep	Harholt	1046:1052	arg1	Curr					1103:1106	Curr	1103:1106	Curr	1103:1106	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	6	15	dep	Harholt	1046:1052	arg1	Mohnen					1095:1100	Mohnen	1095:1100	Mohnen	1095:1100	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	6	15	dep	Harholt	1046:1052	arg1	2008					1136:1139	2008	1136:1139	2008	1136:1139	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	5	16	theme	infection	797:805	arg1	strategy					807:814	their infection strategy	791:814	their infection strategy	791:814	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	5	17	dep	Espino	817:822	arg1	ten					863:865	ten	863:865	ten	863:865	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	5	17	dep	Espino	817:822	arg1	2010					857:860	2010	857:860	2010	857:860	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	5	17	dep	Espino	817:822	arg1	al.					827:829	Espino et al.	817:829	Espino et al.	817:829	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	5	17	dep	Espino	817:822	arg1	Plant-Microbe					884:896	Plant-Microbe	884:896	Plant-Microbe	884:896	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	2	18	theme	Cell	225:228	arg1	walls					230:234	Cell walls	225:234	Cell walls	225:234	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	5	19	theme	pectin-degrading	755:770	arg1	enzymes					772:778	pectin-degrading enzymes	755:778	pectin-degrading enzymes	755:778	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	4	20	theme	acid	602:605	arg1	subunits					607:614	galacturonic acid subunits	589:614	galacturonic acid subunits	589:614	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	9	21	dep	Raiola	1854:1859	arg1	al.					1864:1866	Raiola et al.	1854:1866	Raiola et al.	1854:1866	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	10	22	theme	specific	2272:2279	arg1	components					2288:2297	specific pectin components	2272:2297	specific pectin components	2272:2297	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	2	23	theme	deconstructing	354:367	arg1	wall					384:387	the plant cell wall	369:387	deconstructing the plant cell wall for bioenergy	354:401	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	7	24	dep	Bethke	1271:1276	arg1	Lionetti					1321:1328	Lionetti	1321:1328	Lionetti	1321:1328	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	24	dep	Bethke	1271:1276	arg1	al.					1281:1283	Bethke et al., Plant Physiol 164:1093-1107	1271:1312	al.	1281:1283	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	24	dep	Bethke	1271:1276	arg1	Physiol					1292:1298	Physiol	1292:1298	Physiol	1292:1298	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	24	dep	Bethke	1271:1276	arg1	2014					1315:1318	2014	1315:1318	2014	1315:1318	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	24	dep	Bethke	1271:1276	arg1	2012					1369:1372	2012	1369:1372	2012	1369:1372	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	9	25	dep	affect	1682:1687	arg1	Bethke					1706:1711	Bethke	1706:1711	Bethke	1706:1711	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	25	dep	affect	1682:1687	arg1	2011					1908:1911	2011	1908:1911	2011	1908:1911	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	25	dep	affect	1682:1687	arg1	Interact					2036:2043	Mol Plant-Microbe Interact 16:945-952	2018:2054	Mol Plant-Microbe Interact 16:945-952	2018:2054	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	25	dep	affect	1682:1687	arg1	Cell					1934:1937	Plant Cell 14:2095-2106	1928:1950	Plant Cell 14:2095-2106	1928:1950	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	1	26	theme	cell	101:104	arg1	source					161:166	a source	159:166	a source of signaling molecules that can modulate plant immunity	159:222	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	1	26	theme	cell	101:104	arg1	barrier					126:132	a barrier	124:132	a barrier to pathogen entry	124:150	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	1	26	theme	cell	101:104	arg1	walls					106:110	Plant cell walls	95:110	Plant cell walls	95:110	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	5	27	dep	secrete	747:753	arg1	Espino					817:822	Espino	817:822	Espino	817:822	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	5	27	dep	secrete	747:753	arg1	Proteomics					832:841	Proteomics	832:841	Proteomics	832:841	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	6	28	theme	cell	1009:1012	arg1	wall					1014:1017	the cell wall	1005:1017	the cell wall	1005:1017	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	7	29	from	amount	1188:1193	arg1	wall					1250:1253	the wall	1246:1253	the wall	1246:1253	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	10	30	theme	biology	2138:2144	arg1	laboratory					2146:2155	any molecular biology laboratory	2124:2155	any molecular biology laboratory	2124:2155	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	9	31	theme	Plant	1825:1829	arg1	Cell					1831:1834	Plant Cell 28:537-556	1825:1845	Plant Cell 28:537-556	1825:1845	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	2	32	theme	plant	373:377	arg1	wall					384:387	the plant cell wall	369:387	deconstructing the plant cell wall for bioenergy	354:401	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	0	33	theme	Track	31:35	arg1	Alterations					47:57	Track Cell Wall Alterations	31:57	Track Cell Wall Alterations	31:57	Measuring Pectin Properties to Track Cell Wall Alterations During Plant-Pathogen Interactions.
31041762	10	34	theme	molecular	2128:2136	arg1	laboratory					2146:2155	any molecular biology laboratory	2124:2155	any molecular biology laboratory	2124:2155	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	0	35	theme	Wall	42:45	arg1	Alterations					47:57	Track Cell Wall Alterations	31:57	Track Cell Wall Alterations	31:57	Measuring Pectin Properties to Track Cell Wall Alterations During Plant-Pathogen Interactions.
31041762	7	36	dep	altered	1262:1268	arg1	Bethke					1271:1276	Bethke	1271:1276	Bethke	1271:1276	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	6	37	theme	esterified	962:971	arg1	fashion					973:979	a highly esterified fashion	953:979	a highly esterified fashion	953:979	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	9	38	theme	methylesterification	1643:1662	arg1	alterations					1621:1631	alterations	1621:1631	alterations of pectin methylesterification or pectin content	1621:1680	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	6	39	theme	pectin	1022:1027	arg1	methylesterases					1029:1043	pectin methylesterases	1022:1043	pectin methylesterases	1022:1043	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	9	40	theme	Mol	2018:2020	arg1	Bethke					1706:1711	Bethke	1706:1711	Bethke	1706:1711	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	40	theme	Mol	2018:2020	arg1	Interact					2036:2043	Mol Plant-Microbe Interact 16:945-952	2018:2054	Mol Plant-Microbe Interact 16:945-952	2018:2054	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	8	41	theme	pectin	1440:1445	arg1	properties					1426:1435	mechanical properties	1415:1435	mechanical properties of pectin	1415:1445	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	9	42	dep	Vogel	1959:1963	arg1	al.					1968:1970	Vogel et al., Plant J 40:968-978, 2004	1959:1996	al.	1968:1970	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	42	dep	Vogel	1959:1963	arg1	J					1979:1979	Plant J 40:968-978	1973:1990	Plant J 40:968-978	1973:1990	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	1	43	theme	pathogen	137:144	arg1	entry					146:150	pathogen entry	137:150	pathogen entry	137:150	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	9	44	theme	pectin	1667:1672	arg1	content					1674:1680	pectin content	1667:1680	pectin content	1667:1680	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	3	45	theme	primary	447:453	arg1	wall					460:463	the primary cell wall	443:463	the primary cell wall	443:463	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	7	46	dep	Lionetti	1321:1328	arg1	al.					1333:1335	Lionetti et al., J Plant Physiol 169:1623-1630	1321:1366	al.	1333:1335	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	7	46	dep	Lionetti	1321:1328	arg1	Physiol					1346:1352	Physiol	1346:1352	Physiol	1346:1352	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	9	47	dep	Vogel	1914:1918	arg1	al.					1923:1925	Vogel et al.	1914:1925	Vogel et al.	1914:1925	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	8	48	theme	Pectin	1376:1381	arg1	methylesterifications					1383:1403	Pectin methylesterifications	1376:1403	Pectin methylesterifications	1376:1403	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	8	49	dep	Levesque-Tremblay	1554:1570	arg1	Planta					1580:1585	Planta 242:791-811	1580:1597	Planta 242:791-811	1580:1597	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	8	49	dep	Levesque-Tremblay	1554:1570	arg1	al.					1575:1577	Levesque-Tremblay et al., Planta 242:791-811, 2015	1554:1603	al.	1575:1577	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	4	50	contain	contain	581:587	arg2	pectins					573:579	all pectins	569:579	all pectins	569:579	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	50	contain	contain	581:587	arg1	pectins					573:579	all pectins	569:579	all pectins	569:579	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	50	contain	contain	581:587	arg2	subunits					607:614	galacturonic acid subunits	589:614	galacturonic acid subunits	589:614	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	50	contain	contain	581:587	arg1	backbone					625:632	their backbone	619:632	their backbone	619:632	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	9	51	dep	Bethke	1810:1815	arg1	Cell					1831:1834	Plant Cell 28:537-556	1825:1845	Plant Cell 28:537-556	1825:1845	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	51	dep	Bethke	1810:1815	arg1	al.					1820:1822	Bethke et al., Plant Cell 28:537-556	1810:1845	al.	1820:1822	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	2	52	dep	sugars	270:275	arg1	hemicellulose					301:313	hemicellulose	301:313	hemicellulose	301:313	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	52	dep	sugars	270:275	arg1	cellulose					278:286	cellulose	278:286	cellulose	278:286	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	52	dep	sugars	270:275	arg1	sugars					270:275	three polymeric sugars	254:275	three polymeric sugars: cellulose, pectin, and hemicellulose	254:313	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	52	dep	sugars	270:275	arg1	pectin					289:294	pectin	289:294	pectin	289:294	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	5	53	dep	Plant-Microbe	884:896	arg1	Interact					898:905	Interact	898:905	Interact 11:1009-1016	898:918	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	9	54	theme	Interact	1887:1894	arg1	24:432-440					1896:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	3	55	theme	wall	460:463	arg1	wall					460:463	the primary cell wall	443:463	the primary cell wall	443:463	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	3	55	theme	wall	460:463	arg1	%					438:438	more than 50%	426:438	more than 50% of the primary cell wall	426:463	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	4	56	dep	Mohnen	684:689	arg1	Biol					708:711	Biol	708:711	Biol	708:711	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	57	theme	pectin	557:562	arg1	types					548:552	various types	540:552	various types of pectin	540:562	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	5	58	theme	Many	732:735	arg1	pathogens					737:745	Many pathogens	732:745	Many pathogens	732:745	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	7	59	theme	pectin	1215:1220	arg1	methylesterification					1222:1241	pectin methylesterification	1215:1241	pectin methylesterification	1215:1241	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	9	60	theme	Plant	1721:1725	arg1	24:432-440					1896:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440	1721:1905	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	2	61	dep	consist	236:242	arg1	Recalcitrance					339:351	Biomass Recalcitrance	331:351	Biomass Recalcitrance	331:351	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	61	dep	consist	236:242	arg1	Mohnen					316:321	Mohnen	316:321	Mohnen	316:321	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	1	62	theme	signaling	171:179	arg1	molecules					181:189	signaling molecules	171:189	signaling molecules that can modulate plant immunity	171:222	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	5	63	dep	ten	863:865	arg1	Have					867:870	Have	867:870	Have et al.	867:877	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	8	64	theme	pectin-degrading	1528:1543	arg1	Levesque-Tremblay					1554:1570	Levesque-Tremblay	1554:1570	Levesque-Tremblay	1554:1570	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	8	64	theme	pectin-degrading	1528:1543	arg1	enzymes					1545:1551	pectin-degrading enzymes	1528:1551	pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015)	1528:1604	Pectin methylesterifications influence mechanical properties of pectin, and pectins must be at least partially de-methylesterified to be substrates for pectin-degrading enzymes (Levesque-Tremblay et al., Planta 242:791-811, 2015).
31041762	6	65	dep	de-esterified	988:1000	arg1	Harholt					1046:1052	Harholt	1046:1052	Harholt	1046:1052	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	6	65	dep	de-esterified	988:1000	arg1	2010					1089:1092	2010	1089:1092	2010	1089:1092	Pectin is synthesized in a highly esterified fashion and is de-esterified in the cell wall by pectin methylesterases (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	10	66	theme	pectin	2175:2180	arg1	content					2182:2188	total pectin content	2169:2188	total pectin content	2169:2188	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	3	67	theme	Plant	494:498	arg1	Physiol					500:506	Plant Physiol 107:1129-1138	494:520	Plant Physiol 107:1129-1138	494:520	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	4	68	dep	Harholt	635:641	arg1	Curr					692:695	Curr	692:695	Curr	692:695	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	68	dep	Harholt	635:641	arg1	al.					646:648	Harholt et al.	635:648	Harholt et al.	635:648	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	68	dep	Harholt	635:641	arg1	Physiol					657:663	Physiol	657:663	Physiol	657:663	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	68	dep	Harholt	635:641	arg1	2010					678:681	2010	678:681	2010	678:681	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	4	68	dep	Harholt	635:641	arg1	Mohnen					684:689	Mohnen	684:689	Mohnen	684:689	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	7	69	from	patterns	1203:1210	arg1	wall					1250:1253	the wall	1246:1253	the wall	1246:1253	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	10	70	theme	colorimetric	2198:2209	arg1	assay					2211:2215	a colorimetric assay	2196:2215	a colorimetric assay	2196:2215	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	0	71	theme	Plant-Pathogen	66:79	arg1	Interactions					81:92	Plant-Pathogen Interactions	66:92	Plant-Pathogen Interactions	66:92	Measuring Pectin Properties to Track Cell Wall Alterations During Plant-Pathogen Interactions.
31041762	3	72	dep	Zablackis	476:484	arg1	al.					489:491	Zablackis et al., Plant Physiol 107:1129-1138, 1995	476:526	al.	489:491	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	3	72	dep	Zablackis	476:484	arg1	Physiol					500:506	Plant Physiol 107:1129-1138	494:520	Plant Physiol 107:1129-1138	494:520	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	7	73	theme	plant-pathogen	1150:1163	arg1	interactions					1165:1176	plant-pathogen interactions	1150:1176	plant-pathogen interactions	1150:1176	During plant-pathogen interactions, both the amount and the patterns of pectin methylesterification in the wall can be altered (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012).
31041762	9	74	dep	Physiol	1727:1733	arg1	Lionetti					1756:1763	Lionetti	1756:1763	Lionetti	1756:1763	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	74	dep	Physiol	1727:1733	arg1	Plant-Microbe					1873:1885	Plant-Microbe	1873:1885	Plant-Microbe	1873:1885	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	4	75	theme	galacturonic	589:600	arg1	subunits					607:614	galacturonic acid subunits	589:614	galacturonic acid subunits	589:614	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	3	76	from	pectin	468:473	arg1	Arabidopsis					414:424	Arabidopsis	414:424	Arabidopsis	414:424	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
31041762	10	77	theme	simple	2088:2093	arg1	protocol					2095:2102	a simple protocol	2086:2102	a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components	2086:2297	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	10	78	theme	pectin	2281:2286	arg1	components					2288:2297	specific pectin components	2272:2297	specific pectin components	2272:2297	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	2	79	dep	Mohnen	316:321	arg1	2008					404:407	2008	404:407	2008	404:407	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	79	dep	Mohnen	316:321	arg1	al.					326:328	Mohnen et al.	316:328	Mohnen et al.	316:328	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	79	dep	Mohnen	316:321	arg1	wall					384:387	the plant cell wall	369:387	deconstructing the plant cell wall for bioenergy	354:401	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	4	80	dep	contain	581:587	arg1	Harholt					635:641	Harholt	635:641	Harholt	635:641	There are various types of pectin, but all pectins contain galacturonic acid subunits in their backbone (Harholt et al., Plant Physiol 153:384-395, 2010; Mohnen, Curr Opin Plant Biol 11:266-277, 2008).
31041762	0	81	theme	Pectin	10:15	arg1	Properties					17:26	Pectin Properties	10:26	Pectin Properties	10:26	Measuring Pectin Properties to Track Cell Wall Alterations During Plant-Pathogen Interactions.
31041762	5	82	theme	strategy	807:814	arg1	part					783:786	part	783:786	part of their infection strategy	783:814	Many pathogens secrete pectin-degrading enzymes as part of their infection strategy (Espino et al., Proteomics 10:3020-3034, 2010; ten Have et al., Mol Plant-Microbe Interact 11:1009-1016, 1998).
31041762	2	83	theme	Biomass	331:337	arg1	Recalcitrance					339:351	Biomass Recalcitrance	331:351	Biomass Recalcitrance	331:351	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	83	theme	Biomass	331:337	arg1	Mohnen					316:321	Mohnen	316:321	Mohnen	316:321	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	2	84	theme	cell	379:382	arg1	wall					384:387	the plant cell wall	369:387	deconstructing the plant cell wall for bioenergy	354:401	Cell walls consist mainly of three polymeric sugars: cellulose, pectin, and hemicellulose (Mohnen et al., Biomass Recalcitrance: deconstructing the plant cell wall for bioenergy, 2008).
31041762	0	85	theme	Cell	37:40	arg1	Alterations					47:57	Track Cell Wall Alterations	31:57	Track Cell Wall Alterations	31:57	Measuring Pectin Properties to Track Cell Wall Alterations During Plant-Pathogen Interactions.
31041762	9	86	theme	Plant	1973:1977	arg1	J					1979:1979	Plant J 40:968-978	1973:1990	Plant J 40:968-978	1973:1990	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	87	theme	pectin	1636:1641	arg1	methylesterification					1643:1662	pectin methylesterification	1636:1662	pectin methylesterification	1636:1662	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	10	88	used	used	2116:2119	arg2	protocol					2095:2102	a simple protocol	2086:2102	a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components	2086:2297	This chapter explains a simple protocol that can be used in any molecular biology laboratory to estimate total pectin content using a colorimetric assay and pectin composition using antibodies raised against specific pectin components.
31041762	9	89	theme	Plant-Microbe	2022:2034	arg1	Bethke					1706:1711	Bethke	1706:1711	Bethke	1706:1711	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	9	89	theme	Plant-Microbe	2022:2034	arg1	Interact					2036:2043	Mol Plant-Microbe Interact 16:945-952	2018:2054	Mol Plant-Microbe Interact 16:945-952	2018:2054	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	1	90	theme	plant	209:213	arg1	immunity					215:222	plant immunity	209:222	plant immunity	209:222	Plant cell walls act both as a barrier to pathogen entry and as a source of signaling molecules that can modulate plant immunity.
31041762	9	91	theme	content	1674:1680	arg1	alterations					1621:1631	alterations	1621:1631	alterations of pectin methylesterification or pectin content	1621:1680	Additionally, alterations of pectin methylesterification or pectin content affect pathogen growth (Bethke et al., Plant Physiol 164:1093-1107, 2014; Lionetti et al., J Plant Physiol 169:1623-1630, 2012; Bethke et al., Plant Cell 28:537-556, 2016; Raiola et al., Mol Plant-Microbe Interact 24:432-440, 2011; Vogel et al., Plant Cell 14:2095-2106, 2002; Vogel et al., Plant J 40:968-978, 2004; Wietholter et al., Mol Plant-Microbe Interact 16:945-952, 2003).
31041762	3	92	theme	cell	455:458	arg1	wall					460:463	the primary cell wall	443:463	the primary cell wall	443:463	In Arabidopsis more than 50% of the primary cell wall is pectin (Zablackis et al., Plant Physiol 107:1129-1138, 1995).
29288461	4	0	theme	carbohydrate	872:883	arg1	fingerprinting					885:898	carbohydrate fingerprinting	872:898	carbohydrate fingerprinting	872:898	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	1	theme	modified	917:924	arg1	variants					926:933	modified variants	917:933	modified variants	917:933	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	2	theme	fingerprinting	885:898	arg1	identification					823:836	the identification	819:836	the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions	819:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	0	3	from	Screening	0:8	arg1	Bacteria					71:78	Host Interacting Bacteria	54:78	Host Interacting Bacteria	54:78	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.
29288461	3	4	from	transition	515:524	arg1	association					569:579	an intimate association	557:579	an intimate association with the eukaryotic host	557:604	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	2	5	theme	matrix	295:300	arg1	They					244:247	They	244:247	They	244:247	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	2	5	theme	matrix	295:300	arg1	components					263:272	main components	258:272	main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation	258:374	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	3	6	theme	growth	547:552	arg1	mode					539:542	a biofilm mode	529:542	a biofilm mode of growth	529:552	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	4	7	theme	intracellular	704:716	arg1	level					718:722	the intracellular level	700:722	the intracellular level of c-di-GMP in virtually any gram-negative bacteria	700:774	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	2	8	theme	extracellular	281:293	arg1	matrix					295:300	the extracellular matrix	277:300	the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation	277:374	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	1	9	theme	abiotic	181:187	arg1	factors					235:241	host defensive factors	220:241	host defensive factors	220:241	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	1	9	theme	abiotic	181:187	arg1	stresses					200:207	abiotic and biotic stresses	181:207	abiotic and biotic stresses	181:207	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	3	10	theme	number	403:408	arg1	Biosynthesis					377:388	Biosynthesis	377:388	Biosynthesis of a growing number of EPS	377:415	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	4	11	theme	composition	856:866	arg1	identification					823:836	the identification	819:836	the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions	819:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	3	12	theme	intimate	560:567	arg1	association					569:579	an intimate association	557:579	an intimate association with the eukaryotic host	557:604	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	1	13	theme	biotic	193:198	arg1	factors					235:241	host defensive factors	220:241	host defensive factors	220:241	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	1	13	theme	biotic	193:198	arg1	stresses					200:207	abiotic and biotic stresses	181:207	abiotic and biotic stresses	181:207	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	4	14	theme	monosaccharide	841:854	arg1	composition					856:866	monosaccharide composition	841:866	monosaccharide composition	841:866	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	15	theme	c-di-GMP	727:734	arg1	level					718:722	the intracellular level	700:722	the intracellular level of c-di-GMP in virtually any gram-negative bacteria	700:774	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	0	16	theme	Exopolysaccharides	32:49	arg1	Screening					0:8	Screening	0:8	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.	0:79	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.
29288461	4	17	theme	variants	926:933	arg1	composition					856:866	monosaccharide composition	841:866	monosaccharide composition	841:866	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	17	theme	variants	926:933	arg1	fingerprinting					885:898	carbohydrate fingerprinting	872:898	carbohydrate fingerprinting	872:898	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	2	18	theme	cell-cell	314:322	arg1	recognition					324:334	cell-cell recognition	314:334	cell-cell recognition	314:334	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	3	19	theme	second	469:474	arg1	c-di-GMP					486:493	the ubiquitous second messenger c-di-GMP	454:493	the ubiquitous second messenger c-di-GMP	454:493	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	3	20	theme	growing	395:401	arg1	number					403:408	a growing number	393:408	a growing number of EPS	393:415	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	3	21	theme	biofilm	531:537	arg1	mode					539:542	a biofilm mode	529:542	a biofilm mode of growth	529:552	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	3	22	theme	messenger	476:484	arg1	c-di-GMP					486:493	the ubiquitous second messenger c-di-GMP	454:493	the ubiquitous second messenger c-di-GMP	454:493	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	0	23	theme	Host	54:57	arg1	Bacteria					71:78	Host Interacting Bacteria	54:78	Host Interacting Bacteria	54:78	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.
29288461	1	24	theme	Bacterial	81:89	arg1	exopolysaccharides					91:108	Bacterial exopolysaccharides	81:108	Bacterial exopolysaccharides (EPS)	81:114	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	1	24	theme	Bacterial	81:89	arg1	EPS					111:113	EPS	111:113	EPS	111:113	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	3	25	theme	eukaryotic	590:599	arg1	host					601:604	the eukaryotic host	586:604	the eukaryotic host	586:604	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	1	26	theme	host	220:223	arg1	factors					235:241	host defensive factors	220:241	host defensive factors	220:241	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	4	27	theme	gram-negative	753:765	arg1	bacteria					767:774	virtually any gram-negative bacteria	739:774	virtually any gram-negative bacteria	739:774	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	1	28	theme	defensive	225:233	arg1	factors					235:241	host defensive factors	220:241	host defensive factors	220:241	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	3	29	theme	EPS	413:415	arg1	number					403:408	a growing number	393:408	a growing number of EPS	393:415	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	4	30	theme	EPS	909:911	arg1	composition					856:866	monosaccharide composition	841:866	monosaccharide composition	841:866	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	30	theme	EPS	909:911	arg1	fingerprinting					885:898	carbohydrate fingerprinting	872:898	carbohydrate fingerprinting	872:898	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	2	31	theme	biofilm	358:364	arg1	formation					366:374	biofilm formation	358:374	biofilm formation	358:374	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	0	32	theme	Interacting	59:69	arg1	Bacteria					71:78	Host Interacting Bacteria	54:78	Host Interacting Bacteria	54:78	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.
29288461	4	33	theme	novel	903:907	arg1	EPS					909:911	novel EPS	903:911	novel EPS	903:911	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	1	34	theme	survival	131:138	arg1	advantage					140:148	a survival advantage	129:148	a survival advantage	129:148	Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors.
29288461	4	35	theme	throughput	788:797	arg1	screening					799:807	a high throughput screening	781:807	a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions	781:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	35	theme	throughput	788:797	arg1	HTS					810:812	HTS	810:812	HTS	810:812	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	36	theme	approach	666:673	arg1	combination					648:658	the combination	644:658	the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions	644:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	37	from	level	718:722	arg1	bacteria					767:774	virtually any gram-negative bacteria	739:774	virtually any gram-negative bacteria	739:774	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	38	theme	high	783:786	arg1	screening					799:807	a high throughput screening	781:807	a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions	781:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	4	38	theme	high	783:786	arg1	HTS					810:812	HTS	810:812	HTS	810:812	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	2	39	theme	surface	337:343	arg1	adhesion					345:352	surface adhesion	337:352	surface adhesion	337:352	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	3	40	theme	ubiquitous	458:467	arg1	c-di-GMP					486:493	the ubiquitous second messenger c-di-GMP	454:493	the ubiquitous second messenger c-di-GMP	454:493	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
29288461	2	41	theme	main	258:261	arg1	They					244:247	They	244:247	They	244:247	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	2	41	theme	main	258:261	arg1	components					263:272	main components	258:272	main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation	258:374	They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation.
29288461	4	42	theme	host-bacteria	960:972	arg1	interactions					974:985	host-bacteria interactions	960:985	host-bacteria interactions	960:985	Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions.
29288461	0	43	theme	c-di-GMP-Regulated	13:30	arg1	Exopolysaccharides					32:49	c-di-GMP-Regulated Exopolysaccharides	13:49	c-di-GMP-Regulated Exopolysaccharides	13:49	Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.
29288461	3	44	with	association	569:579	arg1	host					601:604	the eukaryotic host	586:604	the eukaryotic host	586:604	Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host.
30936014	8	0	theme	sponges	1046:1052	arg1	construct					1022:1030	The inside construct	1011:1030	The inside construct of the C/HNTs sponges	1011:1052	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	8	0	theme	sponges	1046:1052	arg1	full					1059:1062	full	1059:1062	full	1059:1062	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	4	1	theme	bromohexadecane	538:552	arg1	solution					562:569	bromohexadecane ethanol solution	538:569	bromohexadecane ethanol solution	538:569	The C/HNTs sponge was modified to be hydrophobic via immersing into bromohexadecane ethanol solution.
30936014	6	2	theme	chitin	795:800	arg1	sponge					802:807	chitin sponge	795:807	chitin sponge	795:807	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	6	3	from	influence	817:825	arg1	structure					837:845	porous structure	830:845	porous structure	830:845	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	12	4	theme	great	1702:1706	arg1	potential					1708:1716	a great potential	1700:1716	a great potential	1700:1716	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	10	5	theme	weight	1479:1484	arg1	times					1387:1391	~11.23 times	1380:1391	~11.23 times of the sponge's weight	1380:1414	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	5	theme	weight	1479:1484	arg1	chloroform					1337:1346	chloroform	1337:1346	chloroform	1337:1346	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	5	theme	weight	1479:1484	arg1	times					1457:1461	~3.94 times	1451:1461	~3.94 times of the sponge's weight	1451:1484	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	5	theme	weight	1479:1484	arg1	absorption					1425:1434	the absorption	1421:1434	the absorption for hexane	1421:1445	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	5	theme	weight	1479:1484	arg1	amount					1327:1332	the total amount	1317:1332	the total amount of chloroform absorbed by a C/HNTs sponge	1317:1374	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	5	6	theme	oil-water	653:661	arg1	ability					674:680	oil-water separation ability	653:680	oil-water separation ability	653:680	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	11	7	theme	excellent	1516:1524	arg1	ability					1547:1553	an excellent oil-water separation ability	1513:1553	an excellent oil-water separation ability	1513:1553	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	7	8	dep	hydrophobicity	959:972	arg1	the					955:957	the	955:957	the	955:957	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	5	9	theme	separation	663:672	arg1	ability					674:680	oil-water separation ability	653:680	oil-water separation ability	653:680	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	2	10	theme	chitin/halloysite	327:343	arg1	composites					364:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	2	11	theme	nanotubes	345:353	arg1	composites					364:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	10	12	theme	weight	1409:1414	arg1	times					1387:1391	~11.23 times	1380:1391	~11.23 times of the sponge's weight	1380:1414	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	12	theme	weight	1409:1414	arg1	chloroform					1337:1346	chloroform	1337:1346	chloroform	1337:1346	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	12	theme	weight	1409:1414	arg1	times					1457:1461	~3.94 times	1451:1461	~3.94 times of the sponge's weight	1451:1484	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	12	theme	weight	1409:1414	arg1	absorption					1425:1434	the absorption	1421:1434	the absorption for hexane	1421:1445	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	12	theme	weight	1409:1414	arg1	amount					1327:1332	the total amount	1317:1332	the total amount of chloroform absorbed by a C/HNTs sponge	1317:1374	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	12	13	contain	have	1695:1698	arg1	sponges					1647:1653	the chitin/HNTs sponges	1631:1653	the chitin/HNTs sponges with improved mechanical property	1631:1687	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	12	13	contain	have	1695:1698	arg2	potential					1708:1716	a great potential	1700:1716	a great potential	1700:1716	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	8	14	theme	pores	1082:1086	arg1	construct					1022:1030	The inside construct	1011:1030	The inside construct of the C/HNTs sponges	1011:1052	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	8	14	theme	pores	1082:1086	arg1	full					1059:1062	full	1059:1062	full	1059:1062	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	12	15	theme	mechanical	1669:1678	arg1	property					1680:1687	improved mechanical property	1660:1687	improved mechanical property	1660:1687	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	9	16	theme	absorption	1138:1147	arg1	capability					1149:1158	The absorption capability	1134:1158	The absorption capability for various oil and grease	1134:1185	The absorption capability for various oil and grease were measured, including methylbenzene, sunflower seed oil, carbon dichloride, n-hexane, chloroform and acetone.
30936014	8	17	theme	interconnected	1067:1080	arg1	pores					1082:1086	interconnected pores	1067:1086	interconnected pores	1067:1086	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	12	18	theme	chitin/HNTs	1635:1645	arg1	sponges					1647:1653	the chitin/HNTs sponges	1631:1653	the chitin/HNTs sponges with improved mechanical property	1631:1687	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	10	19	theme	chloroform	1337:1346	arg1	chloroform					1337:1346	chloroform	1337:1346	chloroform	1337:1346	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	19	theme	chloroform	1337:1346	arg1	times					1387:1391	~11.23 times	1380:1391	~11.23 times of the sponge's weight	1380:1414	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	19	theme	chloroform	1337:1346	arg1	amount					1327:1332	the total amount	1317:1332	the total amount of chloroform absorbed by a C/HNTs sponge	1317:1374	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	1	20	theme	promising	210:218	arg1	device					153:158	A high-performance oil-water separation device	113:158	A high-performance oil-water separation device that built by environment-friendly materials	113:203	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	1	20	theme	promising	210:218	arg1	strategy					220:227	a promising strategy	208:227	a promising strategy to solve water pollution problem	208:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	5	21	theme	C/HNTs	689:694	arg1	sponge					696:701	the C/HNTs sponge	685:701	the C/HNTs sponge	685:701	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	11	22	theme	separation	1577:1586	arg1	efficiency					1588:1597	98.7% separation efficiency	1571:1597	98.7% separation efficiency	1571:1597	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	2	23	theme	separation	304:313	arg1	system					315:320	an oil-water separation system	291:320	an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites	291:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	6	24	theme	compressive	771:781	arg1	strength					783:790	the compressive strength	767:790	the compressive strength of chitin sponge	767:807	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	0	25	theme	modified	16:23	arg1	chitin/halloysite					25:41	Hydrophobically modified chitin/halloysite	0:41	Hydrophobically modified chitin/halloysite	0:41	Hydrophobically modified chitin/halloysite nanotubes composite sponges for high efficiency oil-water separation.
30936014	5	26	theme	sponge	696:701	arg1	structure					584:592	surface structure	576:592	surface structure	576:592	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	5	26	theme	sponge	696:701	arg1	absorption					638:647	oil absorption	634:647	oil absorption	634:647	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	5	26	theme	sponge	696:701	arg1	properties					606:615	mechanical properties	595:615	mechanical properties	595:615	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	5	26	theme	sponge	696:701	arg1	ability					674:680	oil-water separation ability	653:680	oil-water separation ability	653:680	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	5	26	theme	sponge	696:701	arg1	microstructure					618:631	microstructure	618:631	microstructure	618:631	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	5	27	theme	oil	634:636	arg1	absorption					638:647	oil absorption	634:647	oil absorption	634:647	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	9	28	theme	various	1164:1170	arg1	oil					1172:1174	various oil	1164:1174	various oil	1164:1174	The absorption capability for various oil and grease were measured, including methylbenzene, sunflower seed oil, carbon dichloride, n-hexane, chloroform and acetone.
30936014	8	29	theme	pore	1097:1100	arg1	size					1102:1105	the pore size	1093:1105	the pore size	1093:1105	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	1	30	theme	high-performance	115:130	arg1	device					153:158	A high-performance oil-water separation device	113:158	A high-performance oil-water separation device that built by environment-friendly materials	113:203	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	1	30	theme	high-performance	115:130	arg1	strategy					220:227	a promising strategy	208:227	a promising strategy to solve water pollution problem	208:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	5	31	dep	structure	584:592	arg1	The					572:574	The	572:574	The	572:574	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	3	32	theme	freeze-dried	423:434	arg1	sponge					451:456	freeze-dried, then a porous sponge	423:456	sponge	451:456	C/HNTs were crosslinked by epichlorohydrin and freeze-dried, then a porous sponge was formed.
30936014	7	33	theme	bromohexadecane	895:909	arg1	coating					884:890	the successful coating	869:890	the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct	869:1008	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	7	34	theme	C/HNTs	918:923	arg1	surface					932:938	the C/HNTs sponge surface	914:938	the C/HNTs sponge surface	914:938	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	1	35	theme	oil-water	132:140	arg1	device					153:158	A high-performance oil-water separation device	113:158	A high-performance oil-water separation device that built by environment-friendly materials	113:203	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	1	35	theme	oil-water	132:140	arg1	strategy					220:227	a promising strategy	208:227	a promising strategy to solve water pollution problem	208:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	7	36	theme	sponge	925:930	arg1	surface					932:938	the C/HNTs sponge surface	914:938	the C/HNTs sponge surface	914:938	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	1	37	theme	water	238:242	arg1	problem					254:260	water pollution problem	238:260	water pollution problem	238:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	2	38	theme	C/HNTs	356:361	arg1	composites					364:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	7	39	theme	successful	873:882	arg1	coating					884:890	the successful coating	869:890	the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct	869:1008	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	1	40	theme	separation	142:151	arg1	device					153:158	A high-performance oil-water separation device	113:158	A high-performance oil-water separation device that built by environment-friendly materials	113:203	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	1	40	theme	separation	142:151	arg1	strategy					220:227	a promising strategy	208:227	a promising strategy to solve water pollution problem	208:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	6	41	theme	HNTs	738:741	arg1	addition					726:733	The addition	722:733	The addition of HNTs	722:741	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	1	42	theme	pollution	244:252	arg1	problem					254:260	water pollution problem	238:260	water pollution problem	238:260	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	12	43	theme	oil-water	1721:1729	arg1	separation					1731:1740	oil-water separation	1721:1740	oil-water separation	1721:1740	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	12	44	theme	improved	1660:1667	arg1	property					1680:1687	improved mechanical property	1660:1687	improved mechanical property	1660:1687	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	0	45	theme	composite	53:61	arg1	sponges					63:69	composite sponges	53:69	composite sponges	53:69	Hydrophobically modified chitin/halloysite nanotubes composite sponges for high efficiency oil-water separation.
30936014	8	46	dep	500 μm	1126:1131	arg1	to					1123:1124	to	1123:1124	to	1123:1124	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	11	47	theme	98.7	1571:1574	arg1	%					1575:1575	%	1575:1575	%	1575:1575	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	5	48	theme	mechanical	595:604	arg1	properties					606:615	mechanical properties	595:615	mechanical properties	595:615	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	9	49	theme	sunflower	1227:1235	arg1	oil					1242:1244	sunflower seed oil	1227:1244	sunflower seed oil	1227:1244	The absorption capability for various oil and grease were measured, including methylbenzene, sunflower seed oil, carbon dichloride, n-hexane, chloroform and acetone.
30936014	3	50	theme	porous	444:449	arg1	sponge					451:456	freeze-dried, then a porous sponge	423:456	sponge	451:456	C/HNTs were crosslinked by epichlorohydrin and freeze-dried, then a porous sponge was formed.
30936014	0	51	theme	efficiency	80:89	arg1	separation					101:110	high efficiency oil-water separation	75:110	high efficiency oil-water separation	75:110	Hydrophobically modified chitin/halloysite nanotubes composite sponges for high efficiency oil-water separation.
30936014	7	52	theme	IR	848:849	arg1	spectra					851:857	IR spectra	848:857	IR spectra	848:857	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	7	53	from	coating	884:890	arg1	surface					932:938	the C/HNTs sponge surface	914:938	the C/HNTs sponge surface	914:938	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	6	54	theme	porous	830:835	arg1	structure					837:845	porous structure	830:845	porous structure	830:845	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	7	55	theme	construct	1000:1008	arg1	lipophilicity					978:990	lipophilicity	978:990	lipophilicity	978:990	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	7	55	theme	construct	1000:1008	arg1	hydrophobicity					959:972	hydrophobicity	959:972	hydrophobicity	959:972	IR spectra indicated the successful coating of bromohexadecane on the C/HNTs sponge surface, which enabled the hydrophobicity and lipophilicity of this construct.
30936014	11	56	theme	%	1575:1575	arg1	efficiency					1588:1597	98.7% separation efficiency	1571:1597	98.7% separation efficiency	1571:1597	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	2	57	theme	oil-water	294:302	arg1	system					315:320	an oil-water separation system	291:320	an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites	291:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	9	58	theme	carbon	1247:1252	arg1	dichloride					1254:1263	carbon dichloride	1247:1263	carbon dichloride	1247:1263	The absorption capability for various oil and grease were measured, including methylbenzene, sunflower seed oil, carbon dichloride, n-hexane, chloroform and acetone.
30936014	9	59	theme	seed	1237:1240	arg1	oil					1242:1244	sunflower seed oil	1227:1244	sunflower seed oil	1227:1244	The absorption capability for various oil and grease were measured, including methylbenzene, sunflower seed oil, carbon dichloride, n-hexane, chloroform and acetone.
30936014	2	60	with	system	315:320	arg1	composites					364:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	chitin/halloysite nanotubes (C/HNTs) composites	327:373	In this study, we developed an oil-water separation system with chitin/halloysite nanotubes (C/HNTs) composites.
30936014	8	61	theme	inside	1015:1020	arg1	construct					1022:1030	The inside construct	1011:1030	The inside construct of the C/HNTs sponges	1011:1052	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	8	61	theme	inside	1015:1020	arg1	full					1059:1062	full	1059:1062	full	1059:1062	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	8	62	theme	C/HNTs	1039:1044	arg1	sponges					1046:1052	the C/HNTs sponges	1035:1052	the C/HNTs sponges	1035:1052	The inside construct of the C/HNTs sponges were full of interconnected pores, and the pore size ranged from 250 to 500 μm.
30936014	10	63	theme	C/HNTs	1362:1367	arg1	sponge					1369:1374	a C/HNTs sponge	1360:1374	a C/HNTs sponge	1360:1374	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	11	64	theme	separation	1536:1545	arg1	ability					1547:1553	an excellent oil-water separation ability	1513:1553	an excellent oil-water separation ability	1513:1553	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	0	65	theme	oil-water	91:99	arg1	separation					101:110	high efficiency oil-water separation	75:110	high efficiency oil-water separation	75:110	Hydrophobically modified chitin/halloysite nanotubes composite sponges for high efficiency oil-water separation.
30936014	4	66	theme	C/HNTs	474:479	arg1	hydrophobic					507:517	hydrophobic	507:517	hydrophobic	507:517	The C/HNTs sponge was modified to be hydrophobic via immersing into bromohexadecane ethanol solution.
30936014	4	66	theme	C/HNTs	474:479	arg1	sponge					481:486	The C/HNTs sponge	470:486	The C/HNTs sponge	470:486	The C/HNTs sponge was modified to be hydrophobic via immersing into bromohexadecane ethanol solution.
30936014	11	67	theme	oil-water	1526:1534	arg1	ability					1547:1553	an excellent oil-water separation ability	1513:1553	an excellent oil-water separation ability	1513:1553	The sponge also exhibited an excellent oil-water separation ability with as high as 98.7% separation efficiency.
30936014	5	68	theme	surface	576:582	arg1	structure					584:592	surface structure	576:592	surface structure	576:592	The surface structure, mechanical properties, microstructure, oil absorption and oil-water separation ability of the C/HNTs sponge were investigated.
30936014	1	69	theme	environment-friendly	174:193	arg1	materials					195:203	environment-friendly materials	174:203	environment-friendly materials	174:203	A high-performance oil-water separation device that built by environment-friendly materials is a promising strategy to solve water pollution problem.
30936014	0	70	theme	high	75:78	arg1	efficiency					80:89	high efficiency	75:89	high efficiency oil-water separation	75:110	Hydrophobically modified chitin/halloysite nanotubes composite sponges for high efficiency oil-water separation.
30936014	4	71	theme	ethanol	554:560	arg1	solution					562:569	bromohexadecane ethanol solution	538:569	bromohexadecane ethanol solution	538:569	The C/HNTs sponge was modified to be hydrophobic via immersing into bromohexadecane ethanol solution.
30936014	12	72	with	sponges	1647:1653	arg1	property					1680:1687	improved mechanical property	1660:1687	improved mechanical property	1660:1687	All the results suggested that the chitin/HNTs sponges with improved mechanical property would have a great potential in oil-water separation.
30936014	6	73	theme	sponge	802:807	arg1	strength					783:790	the compressive strength	767:790	the compressive strength of chitin sponge	767:807	The addition of HNTs significantly increased the compressive strength of chitin sponge without influence on porous structure.
30936014	10	74	theme	total	1321:1325	arg1	chloroform					1337:1346	chloroform	1337:1346	chloroform	1337:1346	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	74	theme	total	1321:1325	arg1	times					1387:1391	~11.23 times	1380:1391	~11.23 times of the sponge's weight	1380:1414	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
30936014	10	74	theme	total	1321:1325	arg1	amount					1327:1332	the total amount	1317:1332	the total amount of chloroform absorbed by a C/HNTs sponge	1317:1374	It is found that the total amount of chloroform absorbed by a C/HNTs sponge was ~11.23 times of the sponge's weight, and the absorption for hexane was ~3.94 times of the sponge's weight.
29580487	1	0	theme	isolate	139:145	arg1	SPI					148:150	Concentrated soy protein isolate (SPI) - pectin blends	114:167	SPI	148:150	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	4	1	theme	G	556:556	arg1	*					557:557	both a lower G*	543:557	*	557:557	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	5	2	theme	viscoelastic	642:653	arg1	richer					683:688	richer	683:688	richer	683:688	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	5	2	theme	viscoelastic	642:653	arg1	properties					655:664	the viscoelastic properties	638:664	the viscoelastic properties of the blend	638:677	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	2	3	theme	blends	338:343	arg1	properties					300:309	the viscoelastic properties	283:309	the viscoelastic properties of concentrated SPI-pectin blends	283:343	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	4	4	theme	lower	550:554	arg1	*					557:557	both a lower G*	543:557	*	557:557	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	7	5	theme	blend	1005:1009	arg1	properties					987:996	the viscoelastic properties	970:996	the viscoelastic properties of the blend	970:1009	The results can be explained by considering that the viscoelastic properties of the blend are influenced by thermal degradation of the pectin phase.
29580487	5	6	with	material	723:730	arg1	properties					759:768	stable physical phase properties	737:768	stable physical phase properties	737:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	4	7	contain	had	539:541	arg1	SPI					524:526	SPI	524:526	SPI	524:526	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	4	7	contain	had	539:541	arg1	pectin					532:537	pectin	532:537	pectin	532:537	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	4	7	contain	had	539:541	arg2	*					557:557	both a lower G*	543:557	*	557:557	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	5	8	theme	blend	673:677	arg1	richer					683:688	richer	683:688	richer	683:688	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	5	8	theme	blend	673:677	arg1	properties					655:664	the viscoelastic properties	638:664	the viscoelastic properties of the blend	638:677	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	2	9	theme	study	260:264	arg1	objective					242:250	The objective	238:250	The objective of this study	238:264	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	1	10	theme	Concentrated	114:125	arg1	SPI					148:150	Concentrated soy protein isolate (SPI) - pectin blends	114:167	SPI	148:150	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	0	11	theme	simple	87:92	arg1	material					104:111	a simple composite material	85:111	a simple composite material	85:111	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	8	12	dep	lowering	1134:1141	arg1	ii					1130:1131	ii	1130:1131	ii	1130:1131	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	4	13	from	behavior	613:620	arg1	time					625:628	time	625:628	time	625:628	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	3	14	theme	closed	428:433	arg1	rheometer					442:450	A closed cavity rheometer	426:450	A closed cavity rheometer	426:450	A closed cavity rheometer was used to measure these properties under these conditions.
29580487	7	15	theme	thermal	1029:1035	arg1	degradation					1037:1047	thermal degradation	1029:1047	thermal degradation of the pectin phase	1029:1067	The results can be explained by considering that the viscoelastic properties of the blend are influenced by thermal degradation of the pectin phase.
29580487	5	16	theme	simple	706:711	arg1	material					723:730	a simple composite material	704:730	a simple composite material with stable physical phase properties	704:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	5	17	theme	physical	744:751	arg1	properties					759:768	stable physical phase properties	737:768	stable physical phase properties	737:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	2	18	theme	shear-induced	380:392	arg1	structuring					394:404	shear-induced structuring	380:404	shear-induced structuring	380:404	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	5	19	theme	stable	737:742	arg1	properties					759:768	stable physical phase properties	737:768	stable physical phase properties	737:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	7	20	theme	viscoelastic	974:985	arg1	properties					987:996	the viscoelastic properties	970:996	the viscoelastic properties of the blend	970:1009	The results can be explained by considering that the viscoelastic properties of the blend are influenced by thermal degradation of the pectin phase.
29580487	2	21	theme	viscoelastic	287:298	arg1	properties					300:309	the viscoelastic properties	283:309	the viscoelastic properties of concentrated SPI-pectin blends	283:343	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	0	22	theme	Viscoelastic	0:11	arg1	properties					13:22	Viscoelastic properties	0:22	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.	0:112	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	8	23	theme	acid	1124:1127	arg1	lowering					1134:1141	lowering	1134:1141	ii) lowering of the pH	1130:1151	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	23	theme	acid	1124:1127	arg1	redistribution					1169:1182	water redistribution	1163:1182	iii) water redistribution from the SPI towards the pectin phase	1158:1220	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	23	theme	acid	1124:1127	arg1	release					1100:1106	release	1100:1106	: i) release of galacturonic acid	1095:1127	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	4	24	theme	different	603:611	arg1	behavior					613:620	a different behavior	601:620	a different behavior in time	601:628	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	8	25	theme	pectin	1209:1214	arg1	phase					1216:1220	the pectin phase	1205:1220	the pectin phase	1205:1220	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	6	26	theme	weak	899:902	arg1	phase					914:918	a weak dispersed phase	897:918	a weak dispersed phase	897:918	In addition, the G'pectin was much lower compared with the G'SPI and G'SPI-pectin upon cooling, confirming that pectin formed a weak dispersed phase.
29580487	1	27	theme	pectin	155:160	arg1	blends					162:167	Concentrated soy protein isolate (SPI) - pectin blends	114:167	blends	162:167	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	0	28	theme	soy	27:29	arg1	isolate					39:45	soy protein isolate - pectin blends	27:61	isolate	39:45	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	7	29	theme	phase	1063:1067	arg1	degradation					1037:1047	thermal degradation	1029:1047	thermal degradation of the pectin phase	1029:1067	The results can be explained by considering that the viscoelastic properties of the blend are influenced by thermal degradation of the pectin phase.
29580487	0	30	theme	composite	94:102	arg1	material					104:111	a simple composite material	85:111	a simple composite material	85:111	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	3	31	used	used	456:459	arg2	rheometer					442:450	A closed cavity rheometer	426:450	A closed cavity rheometer	426:450	A closed cavity rheometer was used to measure these properties under these conditions.
29580487	4	32	theme	two	581:583	arg1	blend					568:572	the blend	564:572	the blend of the two	564:583	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	0	33	theme	isolate	39:45	arg1	properties					13:22	Viscoelastic properties	0:22	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.	0:112	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	8	34	theme	pH	1150:1151	arg1	lowering					1134:1141	lowering	1134:1141	ii) lowering of the pH	1130:1151	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	34	theme	pH	1150:1151	arg1	redistribution					1169:1182	water redistribution	1163:1182	iii) water redistribution from the SPI towards the pectin phase	1158:1220	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	34	theme	pH	1150:1151	arg1	release					1100:1106	release	1100:1106	: i) release of galacturonic acid	1095:1127	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	4	35	dep	*	557:557	arg1	both					543:546	both	543:546	both	543:546	At 140 °C, SPI and pectin had both a lower G* than the blend of the two and also showed a different behavior in time.
29580487	1	36	theme	fibrous	177:183	arg1	textures					185:192	fibrous textures	177:192	fibrous textures	177:192	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	0	37	theme	protein	31:37	arg1	isolate					39:45	soy protein isolate - pectin blends	27:61	isolate	39:45	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	5	38	theme	composite	713:721	arg1	material					723:730	a simple composite material	704:730	a simple composite material with stable physical phase properties	704:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	5	39	theme	phase	753:757	arg1	properties					759:768	stable physical phase properties	737:768	stable physical phase properties	737:768	Hence, the viscoelastic properties of the blend are richer than those of a simple composite material with stable physical phase properties.
29580487	9	40	theme	effects	1256:1262	arg1	importance					1236:1245	The relative importance	1223:1245	The relative importance of those effects	1223:1262	The relative importance of those effects are evaluated.
29580487	0	41	theme	pectin	49:54	arg1	blends					56:61	soy protein isolate - pectin blends	27:61	blends	56:61	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	6	42	dep	SPI	832:834	arg1	the					826:828	the	826:828	the	826:828	In addition, the G'pectin was much lower compared with the G'SPI and G'SPI-pectin upon cooling, confirming that pectin formed a weak dispersed phase.
29580487	2	43	theme	SPI-pectin	327:336	arg1	blends					338:343	concentrated SPI-pectin blends	314:343	concentrated SPI-pectin blends	314:343	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	2	44	theme	concentrated	314:325	arg1	blends					338:343	concentrated SPI-pectin blends	314:343	concentrated SPI-pectin blends	314:343	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	8	45	dep	release	1100:1106	arg1	i					1097:1097	i	1097:1097	i	1097:1097	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	7	46	theme	pectin	1056:1061	arg1	phase					1063:1067	the pectin phase	1052:1067	the pectin phase	1052:1067	The results can be explained by considering that the viscoelastic properties of the blend are influenced by thermal degradation of the pectin phase.
29580487	8	47	dep	redistribution	1169:1182	arg1	iii					1158:1160	iii	1158:1160	iii	1158:1160	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	9	48	theme	relative	1227:1234	arg1	importance					1236:1245	The relative importance	1223:1245	The relative importance of those effects	1223:1262	The relative importance of those effects are evaluated.
29580487	8	49	theme	water	1163:1167	arg1	redistribution					1169:1182	water redistribution	1163:1182	iii) water redistribution from the SPI towards the pectin phase	1158:1220	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	1	50	theme	protein	131:137	arg1	SPI					148:150	Concentrated soy protein isolate (SPI) - pectin blends	114:167	SPI	148:150	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	8	51	from	SPI	1193:1195	arg1	lowering					1134:1141	lowering	1134:1141	ii) lowering of the pH	1130:1151	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	51	from	SPI	1193:1195	arg1	redistribution					1169:1182	water redistribution	1163:1182	iii) water redistribution from the SPI towards the pectin phase	1158:1220	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	8	51	from	SPI	1193:1195	arg1	release					1100:1106	release	1100:1106	: i) release of galacturonic acid	1095:1127	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	1	52	theme	shear-induced	197:209	arg1	structuring					211:221	shear-induced structuring	197:221	shear-induced structuring while heating	197:235	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	0	53	dep	properties	13:22	arg1	Richer					64:69	Richer	64:69	Richer	64:69	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	1	54	theme	soy	127:129	arg1	SPI					148:150	Concentrated soy protein isolate (SPI) - pectin blends	114:167	SPI	148:150	Concentrated soy protein isolate (SPI) - pectin blends acquire fibrous textures by shear-induced structuring while heating.
29580487	3	55	theme	cavity	435:440	arg1	rheometer					442:450	A closed cavity rheometer	426:450	A closed cavity rheometer	426:450	A closed cavity rheometer was used to measure these properties under these conditions.
29580487	0	56	theme	blends	56:61	arg1	properties					13:22	Viscoelastic properties	0:22	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.	0:112	Viscoelastic properties of soy protein isolate - pectin blends: Richer than those of a simple composite material.
29580487	8	57	theme	galacturonic	1111:1122	arg1	acid					1124:1127	galacturonic acid	1111:1127	galacturonic acid	1111:1127	This degradation leads to: i) release of galacturonic acid, ii) lowering of the pH, and iii) water redistribution from the SPI towards the pectin phase.
29580487	2	58	theme	similar	351:357	arg1	conditions					359:368	similar conditions	351:368	similar conditions as during shear-induced structuring, and after cooling	351:423	The objective of this study was to determine the viscoelastic properties of concentrated SPI-pectin blends under similar conditions as during shear-induced structuring, and after cooling.
29580487	6	59	theme	dispersed	904:912	arg1	phase					914:918	a weak dispersed phase	897:918	a weak dispersed phase	897:918	In addition, the G'pectin was much lower compared with the G'SPI and G'SPI-pectin upon cooling, confirming that pectin formed a weak dispersed phase.
30412759	3	0	with	extraction	545:554	arg1	NaOH					567:570	2.0 M NaOH	561:570	2.0 M NaOH	561:570	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	0	1	with	separation	88:97	arg1	activity					193:200	proliferative activity	179:200	proliferative activity	179:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	1	2	theme	crude	270:274	arg1	β‑glucan					276:283	the crude β‑glucan	266:283	the crude β‑glucan from Saccharomyces cerevisiae	266:313	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	9	3	with	fractions	1585:1593	arg1	polydispersity					1636:1649	low polydispersity	1632:1649	low polydispersity	1632:1649	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	9	3	with	fractions	1585:1593	arg1	weights					1620:1626	different molecular weights	1600:1626	different molecular weights	1600:1626	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	0	4	with	Extraction	73:82	arg1	activity					193:200	proliferative activity	179:200	proliferative activity	179:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	4	5	theme	index	888:892	arg1	detector					894:901	differential refractive index detector	864:901	differential refractive index detector (SEC-MALLS-RI)	864:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	4	5	theme	index	888:892	arg1	SEC-MALLS-RI					904:915	SEC-MALLS-RI	904:915	SEC-MALLS-RI	904:915	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	2	6	theme	spectroscopy	399:410	arg1	analysis					443:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	1	7	theme	yeast	208:212	arg1	β‑glucan					214:221	Pure yeast β‑glucan	203:221	Pure yeast β‑glucan (YG1)	203:227	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	1	7	theme	yeast	208:212	arg1	YG1					224:226	YG1	224:226	YG1	224:226	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	1	8	from	cerevisiae	304:313	arg1	β‑glucan					276:283	the crude β‑glucan	266:283	the crude β‑glucan from Saccharomyces cerevisiae	266:313	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	3	9	with	fractionation	665:677	arg1	NaOH					567:570	2.0 M NaOH	561:570	2.0 M NaOH	561:570	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	9	10	theme	theoretical	1668:1678	arg1	basis					1680:1684	a theoretical basis	1666:1684	a theoretical basis for interpreting the relationship between molecular size and bioactivity	1666:1757	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	3	11	theme	insoluble	592:600	arg1	residue					602:608	the insoluble residue	588:608	the insoluble residue with 1.0 M HCl based on single-factor experiments	588:658	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	7	12	from	31.61 kDa	1182:1190	arg1	weight					1157:1162	molecular weight	1147:1162	molecular weight (Mw) from 4590 to 31.61 kDa	1147:1190	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	7	12	from	31.61 kDa	1182:1190	arg1	index					1215:1219	low polydispersity index	1196:1219	low polydispersity index (Mw/Mn of ~1)	1196:1233	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	7	12	from	31.61 kDa	1182:1190	arg1	Mw					1165:1166	Mw	1165:1166	Mw	1165:1166	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	3	13	theme	weight	473:478	arg1	fractions					480:488	Different molecular weight fractions	453:488	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG)	453:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	5	14	theme	high	952:955	arg1	purity					957:962	a high purity	950:962	a high purity	950:962	Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose.
30412759	8	15	theme	cell	1449:1452	arg1	viability					1454:1462	the highest cell viability	1437:1462	the highest cell viability of 145.8 ± 4.3%	1437:1478	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	4	16	theme	molecular	749:757	arg1	sizes					759:763	The molecular sizes	745:763	The molecular sizes of as-obtained fractions	745:788	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	6	17	theme	WYG	1038:1040	arg1	%					1095:1095	12.41%	1090:1095	12.41%	1090:1095	The WYG yields by alkali extraction and acidolysis were 12.41% and 42.85%, respectively.
30412759	6	17	theme	WYG	1038:1040	arg1	yields					1042:1047	The WYG yields	1034:1047	The WYG yields by alkali extraction and acidolysis	1034:1083	The WYG yields by alkali extraction and acidolysis were 12.41% and 42.85%, respectively.
30412759	5	18	dep	composed	979:986	arg1	%					1009:1009	97.71%	1004:1009	97.71%	1004:1009	Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose.
30412759	8	19	theme	%	1478:1478	arg1	viability					1454:1462	the highest cell viability	1437:1462	the highest cell viability of 145.8 ± 4.3%	1437:1478	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	7	20	theme	low	1196:1198	arg1	index					1215:1219	low polydispersity index	1196:1219	low polydispersity index (Mw/Mn of ~1)	1196:1233	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	4	21	theme	multi-angle	807:817	arg1	scattering					831:840	multi-angle laser light scattering	807:840	multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI)	807:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	2	22	theme	monosaccharide	416:429	arg1	composition					431:441	monosaccharide composition	416:441	monosaccharide composition	416:441	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	0	23	theme	assisted-size	113:125	arg1	chromatography					137:150	acidolysis assisted-size exclusion chromatography	102:150	acidolysis assisted-size exclusion chromatography	102:150	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	7	24	with	fractions	1132:1140	arg1	weight					1157:1162	molecular weight	1147:1162	molecular weight (Mw) from 4590 to 31.61 kDa	1147:1190	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	7	24	with	fractions	1132:1140	arg1	index					1215:1219	low polydispersity index	1196:1219	low polydispersity index (Mw/Mn of ~1)	1196:1233	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	7	24	with	fractions	1132:1140	arg1	Mw					1165:1166	Mw	1165:1166	Mw	1165:1166	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	8	25	theme	1.56 μg/mL	1506:1515	arg1	concentration					1489:1501	a low concentration	1483:1501	a low concentration of 1.56 μg/mL	1483:1515	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	3	26	theme	molecular	463:471	arg1	fractions					480:488	Different molecular weight fractions	453:488	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG)	453:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	0	27	theme	Water-soluble	0:12	arg1	separation					88:97	separation	88:97	separation	88:97	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	27	theme	Water-soluble	0:12	arg1	fractions					29:37	Water-soluble yeast β‑glucan fractions	0:37	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.	0:201	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	27	theme	Water-soluble	0:12	arg1	Extraction					73:82	Extraction	73:82	Extraction	73:82	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	27	theme	Water-soluble	0:12	arg1	association					162:172	their association	156:172	their association with proliferative activity	156:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	1	28	theme	Saccharomyces	290:302	arg1	cerevisiae					304:313	Saccharomyces cerevisiae	290:313	Saccharomyces cerevisiae	290:313	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	7	29	theme	%	1304:1304	arg1	rates					1286:1290	high recovery rates	1272:1290	high recovery rates of 61.9-92.5%	1272:1304	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	4	30	theme	fractions	780:788	arg1	sizes					759:763	The molecular sizes	745:763	The molecular sizes of as-obtained fractions	745:788	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	3	31	theme	β‑glucan	513:520	arg1	fractions					480:488	Different molecular weight fractions	453:488	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG)	453:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	0	32	theme	β‑glucan	20:27	arg1	separation					88:97	separation	88:97	separation	88:97	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	32	theme	β‑glucan	20:27	arg1	fractions					29:37	Water-soluble yeast β‑glucan fractions	0:37	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.	0:201	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	32	theme	β‑glucan	20:27	arg1	Extraction					73:82	Extraction	73:82	Extraction	73:82	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	32	theme	β‑glucan	20:27	arg1	association					162:172	their association	156:172	their association with proliferative activity	156:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	3	33	theme	size	684:687	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	8	34	theme	macrophages	1381:1391	arg1	proliferation					1355:1367	the proliferation	1351:1367	the proliferation of RAW264.7 macrophages	1351:1391	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	3	35	theme	water-soluble	493:505	arg1	WYG					523:525	WYG	523:525	WYG	523:525	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	35	theme	water-soluble	493:505	arg1	β‑glucan					513:520	water-soluble yeast β‑glucan	493:520	water-soluble yeast β‑glucan (WYG)	493:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	7	36	theme	recovery	1277:1284	arg1	rates					1286:1290	high recovery rates	1272:1290	high recovery rates of 61.9-92.5%	1272:1304	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	4	37	theme	differential	864:875	arg1	detector					894:901	differential refractive index detector	864:901	differential refractive index detector (SEC-MALLS-RI)	864:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	4	37	theme	differential	864:875	arg1	SEC-MALLS-RI					904:915	SEC-MALLS-RI	904:915	SEC-MALLS-RI	904:915	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	2	38	theme	content	367:373	arg1	analysis					443:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	0	39	theme	molecular	54:62	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	4	40	theme	light	825:829	arg1	scattering					831:840	multi-angle laser light scattering	807:840	multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI)	807:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	2	41	theme	total	355:359	arg1	content					367:373	total sugar content	355:373	total sugar content	355:373	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	9	42	theme	different	1600:1608	arg1	weights					1620:1626	different molecular weights	1600:1626	different molecular weights	1600:1626	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	3	43	theme	yeast	507:511	arg1	WYG					523:525	WYG	523:525	WYG	523:525	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	43	theme	yeast	507:511	arg1	β‑glucan					513:520	water-soluble yeast β‑glucan	493:520	water-soluble yeast β‑glucan (WYG)	493:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	6	44	theme	alkali	1052:1057	arg1	extraction					1059:1068	alkali extraction	1052:1068	alkali extraction	1052:1068	The WYG yields by alkali extraction and acidolysis were 12.41% and 42.85%, respectively.
30412759	2	45	theme	protein	376:382	arg1	content					384:390	protein content	376:390	protein content	376:390	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	9	46	theme	low	1632:1634	arg1	polydispersity					1636:1649	low polydispersity	1632:1649	low polydispersity	1632:1649	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	7	47	theme	~1	1231:1232	arg1	Mw/Mn					1222:1226	Mw/Mn	1222:1226	Mw/Mn of ~1	1222:1232	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	3	48	from	fractionation	665:677	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	49	from	extraction	545:554	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	50	dep	column	714:719	arg1	SEC					722:724	SEC	722:724	SEC	722:724	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	50	dep	column	714:719	arg1	S-400					737:741	Sephacryl S-400	727:741	Sephacryl S-400	727:741	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	1	51	theme	Pure	203:206	arg1	β‑glucan					214:221	Pure yeast β‑glucan	203:221	Pure yeast β‑glucan (YG1)	203:227	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	1	51	theme	Pure	203:206	arg1	YG1					224:226	YG1	224:226	YG1	224:226	Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae.
30412759	7	52	theme	molecular	1147:1155	arg1	weight					1157:1162	molecular weight	1147:1162	molecular weight (Mw) from 4590 to 31.61 kDa	1147:1190	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	7	52	theme	molecular	1147:1155	arg1	Mw					1165:1166	Mw	1165:1166	Mw	1165:1166	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	4	53	theme	refractive	877:886	arg1	detector					894:901	differential refractive index detector	864:901	differential refractive index detector (SEC-MALLS-RI)	864:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	4	53	theme	refractive	877:886	arg1	SEC-MALLS-RI					904:915	SEC-MALLS-RI	904:915	SEC-MALLS-RI	904:915	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	3	54	theme	residue	602:608	arg1	degradation					573:583	degradation	573:583	degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments	573:658	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	54	theme	residue	602:608	arg1	extraction					545:554	extraction	545:554	extraction with 2.0 M NaOH	545:570	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	54	theme	residue	602:608	arg1	fractionation					665:677	fractionation	665:677	fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400)	665:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	8	55	theme	highest	1441:1447	arg1	viability					1454:1462	the highest cell viability	1437:1462	the highest cell viability of 145.8 ± 4.3%	1437:1478	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	0	56	theme	proliferative	179:191	arg1	activity					193:200	proliferative activity	179:200	proliferative activity	179:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	57	with	association	162:172	arg1	activity					193:200	proliferative activity	179:200	proliferative activity	179:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	9	58	theme	molecular	1728:1736	arg1	size					1738:1741	molecular size	1728:1741	molecular size	1728:1741	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	5	59	contain	had	946:948	arg1	YG1					942:944	YG1	942:944	YG1	942:944	Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose.
30412759	5	59	contain	had	946:948	arg2	purity					957:962	a high purity	950:962	a high purity	950:962	Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose.
30412759	3	60	theme	single-factor	634:646	arg1	experiments					648:658	single-factor experiments	634:658	single-factor experiments	634:658	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	7	61	theme	polydispersity	1200:1213	arg1	index					1215:1219	low polydispersity index	1196:1219	low polydispersity index (Mw/Mn of ~1)	1196:1233	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	8	62	theme	low	1485:1487	arg1	concentration					1489:1501	a low concentration	1483:1501	a low concentration of 1.56 μg/mL	1483:1515	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	3	63	from	degradation	573:583	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	0	64	theme	acidolysis	102:111	arg1	chromatography					137:150	acidolysis assisted-size exclusion chromatography	102:150	acidolysis assisted-size exclusion chromatography	102:150	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	2	65	theme	YG1	320:322	arg1	structure					324:332	The YG1 structure	316:332	The YG1 structure	316:332	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	0	66	theme	exclusion	127:135	arg1	chromatography					137:150	acidolysis assisted-size exclusion chromatography	102:150	acidolysis assisted-size exclusion chromatography	102:150	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	67	theme	yeast	14:18	arg1	separation					88:97	separation	88:97	separation	88:97	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	67	theme	yeast	14:18	arg1	fractions					29:37	Water-soluble yeast β‑glucan fractions	0:37	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.	0:201	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	67	theme	yeast	14:18	arg1	Extraction					73:82	Extraction	73:82	Extraction	73:82	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	67	theme	yeast	14:18	arg1	association					162:172	their association	156:172	their association with proliferative activity	156:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	3	68	theme	chromatography	699:712	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	2	69	theme	FT-IR	393:397	arg1	spectroscopy					399:410	FT-IR spectroscopy	393:410	FT-IR spectroscopy	393:410	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	3	70	with	residue	602:608	arg1	HCl					621:623	1.0 M HCl	615:623	1.0 M HCl based on single-factor experiments	615:658	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	9	71	theme	molecular	1610:1618	arg1	weights					1620:1626	different molecular weights	1600:1626	different molecular weights	1600:1626	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	9	72	theme	efficient	1549:1557	arg1	method					1559:1564	an efficient method	1546:1564	an efficient method for separating WYG fractions with different molecular weights and low polydispersity	1546:1649	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	7	73	dep	index	1215:1219	arg1	Mw/Mn					1222:1226	Mw/Mn	1222:1226	Mw/Mn of ~1	1222:1232	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	4	74	theme	as-obtained	768:778	arg1	fractions					780:788	as-obtained fractions	768:788	as-obtained fractions	768:788	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	0	75	theme	different	44:52	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	7	76	theme	high	1272:1275	arg1	rates					1286:1290	high recovery rates	1272:1290	high recovery rates of 61.9-92.5%	1272:1304	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	3	77	theme	Different	453:461	arg1	fractions					480:488	Different molecular weight fractions	453:488	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG)	453:526	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	8	78	theme	RAW264.7	1372:1379	arg1	macrophages					1381:1391	RAW264.7 macrophages	1372:1391	RAW264.7 macrophages	1372:1391	Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (Mw = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL.
30412759	0	79	with	fractions	29:37	arg1	weights					64:70	different molecular weights	44:70	different molecular weights	44:70	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	2	80	theme	composition	431:441	arg1	analysis					443:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	3	81	with	degradation	573:583	arg1	NaOH					567:570	2.0 M NaOH	561:570	2.0 M NaOH	561:570	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	9	82	theme	WYG	1581:1583	arg1	fractions					1585:1593	WYG fractions	1581:1593	WYG fractions with different molecular weights and low polydispersity	1581:1649	This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
30412759	7	83	dep	31.61 kDa	1182:1190	arg1	to					1179:1180	to	1179:1180	to	1179:1180	Fourteen fractions with molecular weight (Mw) from 4590 to 31.61 kDa and low polydispersity index (Mw/Mn of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%.
30412759	2	84	theme	sugar	361:365	arg1	content					367:373	total sugar content	355:373	total sugar content	355:373	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	0	85	dep	fractions	29:37	arg1	association					162:172	their association	156:172	their association with proliferative activity	156:200	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	85	dep	fractions	29:37	arg1	Extraction					73:82	Extraction	73:82	Extraction	73:82	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	85	dep	fractions	29:37	arg1	fractions					29:37	Water-soluble yeast β‑glucan fractions	0:37	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.	0:201	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	0	85	dep	fractions	29:37	arg1	separation					88:97	separation	88:97	separation	88:97	Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.
30412759	3	86	theme	exclusion	689:697	arg1	column					714:719	a size exclusion chromatography column	682:719	a size exclusion chromatography column (SEC, Sephacryl S-400)	682:742	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	4	87	theme	laser	819:823	arg1	scattering					831:840	multi-angle laser light scattering	807:840	multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI)	807:916	The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI).
30412759	2	88	theme	content	384:390	arg1	analysis					443:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis	355:450	The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis.
30412759	5	89	theme	trace	1019:1023	arg1	mannose					1025:1031	trace mannose	1019:1031	trace mannose	1019:1031	Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose.
30412759	3	90	theme	Sephacryl	727:735	arg1	SEC					722:724	SEC	722:724	SEC	722:724	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	90	theme	Sephacryl	727:735	arg1	S-400					737:741	Sephacryl S-400	727:741	Sephacryl S-400	727:741	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
30412759	3	91	theme	2.0 M	561:565	arg1	NaOH					567:570	2.0 M NaOH	561:570	2.0 M NaOH	561:570	Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400).
29782809	0	0	theme	Chondroitin	71:81	arg1	Sulfate					83:89	Chondroitin Sulfate	71:89	Chondroitin Sulfate	71:89	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	6	1	theme	neuronal	820:827	arg1	density					829:835	neuronal density	820:835	neuronal density	820:835	There were no detectable differences in neuronal density.
29782809	3	2	theme	glial	472:476	arg1	markers					478:484	neuronal and glial markers	459:484	neuronal and glial markers	459:484	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	6	3	theme	detectable	794:803	arg1	differences					805:815	no detectable differences	791:815	no detectable differences	791:815	There were no detectable differences in neuronal density.
29782809	8	4	theme	other	1020:1024	arg1	regions					1026:1032	other regions	1020:1032	other regions examined	1020:1041	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	0	5	from	Effects	4:10	arg1	Accumulation					40:51	Regional Accumulation	31:51	Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain	31:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	2	6	theme	HA-associated	309:321	arg1	members					323:329	HA-associated members	309:329	HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs)	309:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	11	7	theme	increased	1347:1355	arg1	activation					1363:1372	increased glial activation	1347:1372	increased glial activation	1347:1372	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	8	theme	ECM	1266:1268	arg1	accumulation					1250:1261	accumulation	1250:1261	accumulation of ECM	1250:1268	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	8	9	theme	brain	940:944	arg1	relative					946:953	aged brain relative	935:953	aged brain relative to young brain	935:968	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	11	10	theme	age-related	1395:1405	arg1	differences					1416:1426	age-related regional differences	1395:1426	age-related regional differences in vulnerability to injury and ischemia	1395:1466	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	3	11	theme	HA	507:508	arg1	markers					478:484	neuronal and glial markers	459:484	neuronal and glial markers	459:484	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	11	theme	HA	507:508	arg1	density					450:456	microvascular density	436:456	microvascular density	436:456	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	11	theme	HA	507:508	arg1	accumulation					491:502	accumulation	491:502	accumulation of HA and CSPGs	491:518	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	11	12	theme	glial	1357:1361	arg1	activation					1363:1372	increased glial activation	1347:1372	increased glial activation	1347:1372	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	0	13	theme	Sulfate	83:89	arg1	Accumulation					40:51	Regional Accumulation	31:51	Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain	31:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	0	14	from	Accumulation	40:51	arg1	Brain					118:122	the Mouse Brain	108:122	the Mouse Brain	108:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	8	15	theme	aged	935:938	arg1	relative					946:953	aged brain relative	935:953	aged brain relative to young brain	935:968	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	4	16	theme	aged	662:665	arg1	brains					682:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	4	17	dep	middle-aged	633:643	arg1	months					649:654	14 months	646:654	14 months	646:654	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	4	18	theme	middle-aged	633:643	arg1	brains					682:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	5	19	theme	cerebral	739:746	arg1	cortex					748:753	cerebral cortex	739:753	cerebral cortex	739:753	Microvascular density decreased in cerebral cortex and cerebellum with age.
29782809	4	20	theme	young	615:619	arg1	brains					682:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	young (4 months), middle-aged (14 months), and aged (24-26 months) brains	615:687	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	2	21	theme	chondroitin	357:367	arg1	proteoglycans					377:389	chondroitin sulfate proteoglycans	357:389	chondroitin sulfate proteoglycans (CSPGs)	357:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	21	theme	chondroitin	357:367	arg1	CSPGs					392:396	CSPGs	392:396	CSPGs	392:396	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	8	22	theme	HA	905:906	arg1	accumulation					908:919	HA accumulation	905:919	HA accumulation	905:919	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	7	23	with	hippocampus	881:891	arg1	aging					898:902	aging	898:902	aging	898:902	There was an increase in astrocytes in the hippocampus with aging.
29782809	3	24	theme	mouse	523:527	arg1	brains					529:534	mouse brains	523:534	mouse brains	523:534	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	11	25	theme	aging	1277:1281	arg1	brain					1283:1287	the aging brain	1273:1287	the aging brain	1273:1287	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	2	26	theme	family	347:352	arg1	members					323:329	HA-associated members	309:329	HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs)	309:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	26	theme	family	347:352	arg1	HA					301:302	HA	301:302	HA	301:302	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	26	theme	family	347:352	arg1	hyaluronan					289:298	hyaluronan	289:298	hyaluronan (HA)	289:303	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	5	27	theme	Microvascular	704:716	arg1	density					718:724	Microvascular density	704:724	Microvascular density	704:724	Microvascular density decreased in cerebral cortex and cerebellum with age.
29782809	8	28	theme	young	958:962	arg1	brain					964:968	young brain	958:968	young brain	958:968	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	11	29	theme	regional	1407:1414	arg1	differences					1416:1426	age-related regional differences	1395:1426	age-related regional differences in vulnerability to injury and ischemia	1395:1466	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	10	30	theme	neuronal	1169:1176	arg1	bodies					1183:1188	neuronal cell bodies	1169:1188	neuronal cell bodies	1169:1188	HA and CSPGs colocalized with a subset of neuronal cell bodies and astrocytes, and at the microvasculature.
29782809	2	31	theme	lectican	338:345	arg1	family					347:352	the lectican family	334:352	the lectican family of chondroitin sulfate proteoglycans (CSPGs)	334:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	0	32	theme	Normal	15:20	arg1	Aging					22:26	Normal Aging	15:26	Normal Aging	15:26	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	4	33	dep	young	615:619	arg1	months					624:629	4 months	622:629	4 months	622:629	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	0	34	dep	Hyaluronan	56:65	arg1	Proteoglycans					91:103	Proteoglycans	91:103	Proteoglycans	91:103	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	4	35	theme	dentate	582:588	arg1	gyrus					590:594	dentate gyrus	582:594	dentate gyrus	582:594	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	0	36	theme	Mouse	112:116	arg1	Brain					118:122	the Mouse Brain	108:122	the Mouse Brain	108:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	4	37	dep	aged	662:665	arg1	months					674:679	24-26 months	668:679	24-26 months	668:679	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	3	38	theme	microvascular	436:448	arg1	density					450:456	microvascular density	436:456	microvascular density	436:456	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	39	theme	regional	412:419	arg1	differences					421:431	regional differences	412:431	regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains	412:534	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	1	40	theme	normal	174:179	arg1	aging					181:185	normal aging	174:185	normal aging	174:185	The brain changes in volume and composition with normal aging.
29782809	2	41	theme	proteoglycans	377:389	arg1	family					347:352	the lectican family	334:352	the lectican family of chondroitin sulfate proteoglycans (CSPGs)	334:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	0	42	theme	Aging	22:26	arg1	Effects					4:10	The Effects	0:10	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain	0:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	8	43	theme	cerebral	977:984	arg1	cortex					986:991	the cerebral cortex	973:991	the cerebral cortex	973:991	HA accumulation was higher in aged brain relative to young brain in the cerebral cortex and cerebellum, but not in other regions examined.
29782809	2	44	theme	brain	215:219	arg1	components					197:206	Cellular components	188:206	Cellular components of the brain	188:219	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	10	45	theme	astrocytes	1194:1203	arg1	subset					1159:1164	a subset	1157:1164	a subset of neuronal cell bodies and astrocytes	1157:1203	HA and CSPGs colocalized with a subset of neuronal cell bodies and astrocytes, and at the microvasculature.
29782809	2	46	theme	sulfate	369:375	arg1	proteoglycans					377:389	chondroitin sulfate proteoglycans	357:389	chondroitin sulfate proteoglycans (CSPGs)	357:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	46	theme	sulfate	369:375	arg1	CSPGs					392:396	CSPGs	392:396	CSPGs	392:396	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	9	47	theme	brain	1103:1107	arg1	regions					1109:1115	the brain regions	1099:1115	the brain regions examined	1099:1124	In contrast, CSPGs did not change with aging in any of the brain regions examined.
29782809	3	48	from	differences	421:431	arg1	markers					478:484	neuronal and glial markers	459:484	neuronal and glial markers	459:484	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	48	from	differences	421:431	arg1	density					450:456	microvascular density	436:456	microvascular density	436:456	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	48	from	differences	421:431	arg1	accumulation					491:502	accumulation	491:502	accumulation of HA and CSPGs	491:518	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	48	from	differences	421:431	arg1	brains					529:534	mouse brains	523:534	mouse brains	523:534	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	49	theme	neuronal	459:466	arg1	markers					478:484	neuronal and glial markers	459:484	neuronal and glial markers	459:484	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	0	50	theme	Regional	31:38	arg1	Accumulation					40:51	Regional Accumulation	31:51	Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain	31:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	3	51	theme	normal	543:548	arg1	aging					550:554	normal aging	543:554	normal aging	543:554	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	10	52	theme	bodies	1183:1188	arg1	subset					1159:1164	a subset	1157:1164	a subset of neuronal cell bodies and astrocytes	1157:1203	HA and CSPGs colocalized with a subset of neuronal cell bodies and astrocytes, and at the microvasculature.
29782809	11	53	from	differences	1416:1426	arg1	vulnerability					1431:1443	vulnerability	1431:1443	vulnerability to injury and ischemia	1431:1466	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	54	theme	density	1332:1338	arg1	setting					1297:1303	the setting	1293:1303	the setting of decreased microvascular density and/or increased glial activation	1293:1372	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	2	55	theme	Cellular	188:195	arg1	components					197:206	Cellular components	188:206	Cellular components of the brain	188:219	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	56	theme	extracellular	241:253	arg1	ECM					263:265	ECM	263:265	ECM	263:265	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	2	56	theme	extracellular	241:253	arg1	matrix					255:260	an extracellular matrix	238:260	an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs)	238:397	Cellular components of the brain are supported by an extracellular matrix (ECM) comprised largely of hyaluronan (HA) and HA-associated members of the lectican family of chondroitin sulfate proteoglycans (CSPGs).
29782809	10	57	theme	cell	1178:1181	arg1	bodies					1183:1188	neuronal cell bodies	1169:1188	neuronal cell bodies	1169:1188	HA and CSPGs colocalized with a subset of neuronal cell bodies and astrocytes, and at the microvasculature.
29782809	3	58	theme	CSPGs	514:518	arg1	markers					478:484	neuronal and glial markers	459:484	neuronal and glial markers	459:484	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	58	theme	CSPGs	514:518	arg1	density					450:456	microvascular density	436:456	microvascular density	436:456	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	3	58	theme	CSPGs	514:518	arg1	accumulation					491:502	accumulation	491:502	accumulation of HA and CSPGs	491:518	We examined regional differences in microvascular density, neuronal and glial markers, and accumulation of HA and CSPGs in mouse brains during normal aging.
29782809	11	59	from	Differences	1235:1245	arg1	brain					1283:1287	the aging brain	1273:1287	the aging brain	1273:1287	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	59	from	Differences	1235:1245	arg1	setting					1297:1303	the setting	1293:1303	the setting of decreased microvascular density and/or increased glial activation	1293:1372	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	59	from	Differences	1235:1245	arg1	accumulation					1250:1261	accumulation	1250:1261	accumulation of ECM	1250:1268	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	60	theme	decreased	1308:1316	arg1	density					1332:1338	decreased microvascular density	1308:1338	decreased microvascular density	1308:1338	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	4	61	theme	brains	682:687	arg1	gyrus					590:594	dentate gyrus	582:594	dentate gyrus	582:594	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	4	61	theme	brains	682:687	arg1	cortex					561:566	The cortex	557:566	The cortex	557:566	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	4	61	theme	brains	682:687	arg1	hippocampus					569:579	hippocampus	569:579	hippocampus	569:579	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	4	61	theme	brains	682:687	arg1	cerebellum					601:610	cerebellum	601:610	cerebellum	601:610	The cortex, hippocampus, dentate gyrus, and cerebellum of young (4 months), middle-aged (14 months), and aged (24-26 months) brains were analyzed.
29782809	0	62	theme	Hyaluronan	56:65	arg1	Accumulation					40:51	Regional Accumulation	31:51	Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain	31:122	The Effects of Normal Aging on Regional Accumulation of Hyaluronan and Chondroitin Sulfate Proteoglycans in the Mouse Brain.
29782809	7	63	from	increase	851:858	arg1	astrocytes					863:872	astrocytes	863:872	astrocytes	863:872	There was an increase in astrocytes in the hippocampus with aging.
29782809	11	64	theme	microvascular	1318:1330	arg1	density					1332:1338	decreased microvascular density	1308:1338	decreased microvascular density	1308:1338	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
29782809	11	65	theme	activation	1363:1372	arg1	setting					1297:1303	the setting	1293:1303	the setting of decreased microvascular density and/or increased glial activation	1293:1372	Differences in accumulation of ECM in the aging brain, in the setting of decreased microvascular density and/or increased glial activation, might contribute to age-related regional differences in vulnerability to injury and ischemia.
31500063	0	0	theme	localized	81:89	arg1	therapy					98:104	localized cancer therapy	81:104	localized cancer therapy	81:104	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	1	1	theme	cancer	344:349	arg1	inhibition					317:326	the inhibition	313:326	the inhibition	313:326	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	1	1	theme	cancer	344:349	arg1	apoptosis					331:339	apoptosis	331:339	apoptosis	331:339	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	6	2	theme	sustaining	1333:1342	arg1	release					1349:1355	the sustaining drug release	1329:1355	the sustaining drug release for a month	1329:1367	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	3	3	theme	X-ray	1002:1006	arg1	XRD					1024:1026	XRD	1024:1026	XRD	1024:1026	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	3	theme	X-ray	1002:1006	arg1	diffractometry					1008:1021	X-ray diffractometry	1002:1021	X-ray diffractometry (XRD)	1002:1027	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	4	theme	infrared	933:940	arg1	spectroscopy					949:960	infrared (FTIR) spectroscopy	933:960	infrared (FTIR) spectroscopy for chemical bonding of composites	933:995	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	5	theme	dynamic	477:483	arg1	erosion					485:491	the dynamic erosion	473:491	the dynamic erosion of blood flow	473:505	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	6	theme	deposition	707:716	arg1	mechanism					718:726	the deposition mechanism	703:726	the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure	703:1049	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	1	7	theme	Ti	180:181	arg1	alloy					183:187	the Ti alloy	176:187	the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer	176:349	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	3	8	theme	surface	895:901	arg1	morphology					903:912	surface morphology	895:912	surface morphology	895:912	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	1	9	theme	drugs	271:275	arg1	release					260:266	release	260:266	release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer	260:349	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	3	10	theme	crystal	1033:1039	arg1	structure					1041:1049	crystal structure	1033:1049	crystal structure	1033:1049	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	6	11	theme	medication	1594:1603	arg1	medication					1594:1603	medication	1594:1603	medication	1594:1603	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	6	11	theme	medication	1594:1603	arg1	amount					1584:1589	the amount	1580:1589	the amount of medication needed for future clinical applications	1580:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	3	12	theme	scanning	847:854	arg1	loading					811:817	doxorubicin loading	799:817	doxorubicin loading	799:817	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	12	theme	scanning	847:854	arg1	FESEM					877:881	FESEM	877:881	FESEM	877:881	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	12	theme	scanning	847:854	arg1	microscope					865:874	field emission scanning electron microscope	832:874	field emission scanning electron microscope (FESEM)	832:882	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	0	13	theme	cancer	91:96	arg1	therapy					98:104	localized cancer therapy	81:104	localized cancer therapy	81:104	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	2	14	theme	deposition	589:598	arg1	way					600:602	the electrochemical deposition way	569:602	the electrochemical deposition way	569:602	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	15	theme	electron	856:863	arg1	loading					811:817	doxorubicin loading	799:817	doxorubicin loading	799:817	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	15	theme	electron	856:863	arg1	FESEM					877:881	FESEM	877:881	FESEM	877:881	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	15	theme	electron	856:863	arg1	microscope					865:874	field emission scanning electron microscope	832:874	field emission scanning electron microscope (FESEM)	832:882	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	6	16	theme	high	1389:1392	arg1	porosity					1394:1401	high porosity	1389:1401	high porosity	1389:1401	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	0	17	from	coatings	50:57	arg1	alloy					71:75	titanium alloy	62:75	titanium alloy for localized cancer therapy	62:104	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	5	18	theme	hydroxyapatite-coated	1236:1256	arg1	alloy					1276:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	6	19	theme	doxorubicin	1514:1524	arg1	drug					1533:1536	load doxorubicin cancer drug	1509:1536	load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications	1509:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	2	20	theme	flow	502:505	arg1	erosion					485:491	the dynamic erosion	473:491	the dynamic erosion of blood flow	473:505	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	5	21	theme	specimen	1267:1274	arg1	alloy					1276:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	6	22	with	latter	1377:1382	arg1	porosity					1394:1401	high porosity	1389:1401	high porosity	1389:1401	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	2	23	theme	alloy	452:456	arg1	surface					458:464	the alloy surface	448:464	the alloy surface	448:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	6	24	theme	practical	1492:1500	arg1	way					1502:1504	a practical way	1490:1504	a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications	1490:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	6	24	theme	practical	1492:1500	arg1	method					1480:1485	this electrochemical method	1459:1485	this electrochemical method	1459:1485	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	2	25	from	surface	458:464	arg1	exfoliation					389:399	exfoliation	389:399	exfoliation of medicine composite and HA agglomerates from the alloy surface	389:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	2	25	from	surface	458:464	arg1	agglomerates					430:441	medicine composite and HA agglomerates	404:441	medicine composite and HA agglomerates from the alloy surface	404:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	4	26	theme	cell	1056:1059	arg1	culture					1061:1067	The cell culture	1052:1067	The cell culture	1052:1067	The cell culture was carried out to analyze the drug efficacy on cell viability.
31500063	2	27	theme	surface	545:551	arg1	modification					553:564	the additional surface modification	530:564	the additional surface modification by the electrochemical deposition way	530:602	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	28	dep	Fourier	915:921	arg1	transform					923:931	transform	923:931	transform infrared (FTIR) spectroscopy for chemical bonding of composites	923:995	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	29	theme	chemical	966:973	arg1	bonding					975:981	chemical bonding	966:981	chemical bonding of composites	966:995	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	30	theme	HA	427:428	arg1	agglomerates					430:441	medicine composite and HA agglomerates	404:441	medicine composite and HA agglomerates from the alloy surface	404:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	2	31	theme	agglomerates	430:441	arg1	exfoliation					389:399	exfoliation	389:399	exfoliation of medicine composite and HA agglomerates from the alloy surface	389:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	32	theme	visible	766:772	arg1	spectrophotometer					744:760	spectrophotometer	744:760	spectrophotometer (UV visible spectrometer)	744:786	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	32	theme	visible	766:772	arg1	spectrometer					774:785	UV visible spectrometer	763:785	UV visible spectrometer	763:785	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	33	theme	additional	534:543	arg1	modification					553:564	the additional surface modification	530:564	the additional surface modification by the electrochemical deposition way	530:602	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	0	34	theme	composite	40:48	arg1	coatings					50:57	hydroxyapatite composite coatings	25:57	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.	0:105	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	5	35	theme	titanium	1258:1265	arg1	alloy					1276:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	4	36	theme	cell	1117:1120	arg1	viability					1122:1130	cell viability	1117:1130	cell viability	1117:1130	The cell culture was carried out to analyze the drug efficacy on cell viability.
31500063	2	37	theme	composite	413:421	arg1	agglomerates					430:441	medicine composite and HA agglomerates	404:441	medicine composite and HA agglomerates from the alloy surface	404:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	0	38	theme	hydroxyapatite	25:38	arg1	coatings					50:57	hydroxyapatite composite coatings	25:57	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.	0:105	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	6	39	theme	drug	1419:1422	arg1	loading					1424:1430	the drug loading	1415:1430	the drug loading	1415:1430	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	5	40	theme	uncoated	1223:1230	arg1	alloy					1276:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	the uncoated and hydroxyapatite-coated titanium specimen alloy	1219:1280	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	6	41	theme	clinical	1623:1630	arg1	applications					1632:1643	future clinical applications	1616:1643	future clinical applications	1616:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	6	42	theme	electrochemical	1464:1478	arg1	way					1502:1504	a practical way	1490:1504	a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications	1490:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	6	42	theme	electrochemical	1464:1478	arg1	method					1480:1485	this electrochemical method	1459:1485	this electrochemical method	1459:1485	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	3	43	theme	composites	986:995	arg1	bonding					975:981	chemical bonding	966:981	chemical bonding of composites	966:995	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	1	44	theme	side	233:236	arg1	effects					238:244	the side effects	229:244	the side effects	229:244	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	5	45	theme	doxorubicin-chitosan	1154:1173	arg1	composites					1175:1184	doxorubicin-chitosan composites	1154:1184	doxorubicin-chitosan composites	1154:1184	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	2	46	theme	electrochemical	573:587	arg1	way					600:602	the electrochemical deposition way	569:602	the electrochemical deposition way	569:602	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	47	theme	electrochemical	650:664	arg1	reactions					666:674	electrochemical reactions	650:674	electrochemical reactions	650:674	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	48	dep	infrared	933:940	arg1	FTIR					943:946	FTIR	943:946	FTIR	943:946	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	1	49	theme	doxorubicin-chitosan	111:130	arg1	composite					132:140	The doxorubicin-chitosan composite	107:140	The doxorubicin-chitosan composite	107:140	The doxorubicin-chitosan composite is deposited electrochemically on the Ti alloy post hydroxyapatite coated for reducing the side effects by sustaining release of drugs localized near the tumor to achieve the inhibition or apoptosis of cancer.
31500063	3	50	theme	doxorubicin	799:809	arg1	microscope					865:874	field emission scanning electron microscope	832:874	field emission scanning electron microscope (FESEM)	832:882	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	50	theme	doxorubicin	799:809	arg1	loading					811:817	doxorubicin loading	799:817	doxorubicin loading	799:817	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	51	theme	UV	763:764	arg1	spectrophotometer					744:760	spectrophotometer	744:760	spectrophotometer (UV visible spectrometer)	744:786	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	51	theme	UV	763:764	arg1	spectrometer					774:785	UV visible spectrometer	763:785	UV visible spectrometer	763:785	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	52	from	exfoliation	389:399	arg1	surface					458:464	the alloy surface	448:464	the alloy surface	448:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	6	53	theme	cancer	1526:1531	arg1	drug					1533:1536	load doxorubicin cancer drug	1509:1536	load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications	1509:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	3	54	theme	field	832:836	arg1	loading					811:817	doxorubicin loading	799:817	doxorubicin loading	799:817	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	54	theme	field	832:836	arg1	FESEM					877:881	FESEM	877:881	FESEM	877:881	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	54	theme	field	832:836	arg1	microscope					865:874	field emission scanning electron microscope	832:874	field emission scanning electron microscope (FESEM)	832:882	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	6	55	theme	drug	1344:1347	arg1	release					1349:1355	the sustaining drug release	1329:1355	the sustaining drug release for a month	1329:1367	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	6	56	theme	future	1616:1621	arg1	applications					1632:1643	future clinical applications	1616:1643	future clinical applications	1616:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	4	57	theme	drug	1100:1103	arg1	efficacy					1105:1112	the drug efficacy	1096:1112	the drug efficacy on cell viability	1096:1130	The cell culture was carried out to analyze the drug efficacy on cell viability.
31500063	3	58	theme	emission	838:845	arg1	loading					811:817	doxorubicin loading	799:817	doxorubicin loading	799:817	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	58	theme	emission	838:845	arg1	FESEM					877:881	FESEM	877:881	FESEM	877:881	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	3	58	theme	emission	838:845	arg1	microscope					865:874	field emission scanning electron microscope	832:874	field emission scanning electron microscope (FESEM)	832:882	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	59	theme	blood	496:500	arg1	flow					502:505	blood flow	496:505	blood flow	496:505	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	2	60	theme	medicine	404:411	arg1	agglomerates					430:441	medicine composite and HA agglomerates	404:441	medicine composite and HA agglomerates from the alloy surface	404:464	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	3	61	theme	cathodic	609:616	arg1	tests					631:635	The cathodic polarization tests	605:635	The cathodic polarization tests coupled with electrochemical reactions	605:674	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	5	62	theme	electrochemical	1285:1299	arg1	methods					1301:1307	electrochemical methods	1285:1307	electrochemical methods	1285:1307	It is concluded that doxorubicin-chitosan composites can be successfully deposited on the uncoated and hydroxyapatite-coated titanium specimen alloy by electrochemical methods.
31500063	6	63	theme	load	1509:1512	arg1	drug					1533:1536	load doxorubicin cancer drug	1509:1536	load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications	1509:1643	Both have revealed the sustaining drug release for a month and the latter with high porosity can enhance the drug loading to 37.46 μg/cm2, revealing this electrochemical method is a practical way to load doxorubicin cancer drug releasing locally to significantly reduce the amount of medication needed for future clinical applications.
31500063	3	64	theme	polarization	618:629	arg1	tests					631:635	The cathodic polarization tests	605:635	The cathodic polarization tests coupled with electrochemical reactions	605:674	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	0	65	theme	titanium	62:69	arg1	alloy					71:75	titanium alloy	62:75	titanium alloy for localized cancer therapy	62:104	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	0	66	dep	Doxorubicin	0:10	arg1	coatings					50:57	hydroxyapatite composite coatings	25:57	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.	0:105	Doxorubicin - chitosan - hydroxyapatite composite coatings on titanium alloy for localized cancer therapy.
31500063	3	67	theme	doxorubicin	731:741	arg1	mechanism					718:726	the deposition mechanism	703:726	the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure	703:1049	The cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, spectrophotometer (UV visible spectrometer) to measure doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe surface morphology, Fourier transform infrared (FTIR) spectroscopy for chemical bonding of composites, and X-ray diffractometry (XRD) for crystal structure.
31500063	2	68	theme	danger	371:376	arg1	possibility					356:366	The possibility	352:366	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow	352:505	The possibility of danger in case of exfoliation of medicine composite and HA agglomerates from the alloy surface due to the dynamic erosion of blood flow could be overcome with the additional surface modification by the electrochemical deposition way.
31500063	4	69	from	efficacy	1105:1112	arg1	viability					1122:1130	cell viability	1117:1130	cell viability	1117:1130	The cell culture was carried out to analyze the drug efficacy on cell viability.
29380749	7	0	theme	native	1131:1136	arg1	glycosaminoglycans					1174:1191	glycosaminoglycans	1174:1191	glycosaminoglycans	1174:1191	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	7	0	theme	native	1131:1136	arg1	cues					1147:1150	native chemical cues	1131:1150	native chemical cues such as collagens and glycosaminoglycans	1131:1191	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	7	0	theme	native	1131:1136	arg1	collagens					1160:1168	collagens	1160:1168	collagens	1160:1168	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	9	1	theme	hydrogel	1425:1432	arg1	injection					1434:1442	hydrogel injection	1425:1442	hydrogel injection into a rat cervical contusion model	1425:1478	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	6	2	theme	compositional	910:922	arg1	properties					924:933	multiple physical and compositional properties	888:933	multiple physical and compositional properties of this novel material	888:956	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	9	3	with	compatible	1375:1384	arg1	cells					1403:1407	rat Schwann cells	1391:1407	rat Schwann cells in culture	1391:1418	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	2	4	theme	acellular	248:256	arg1	grafts					275:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	11	5	theme	simultaneous	1994:2005	arg1	therapy					2007:2013	a simultaneous therapy	1992:2013	a simultaneous therapy	1992:2013	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	6	6	theme	physical	897:904	arg1	properties					924:933	multiple physical and compositional properties	888:933	multiple physical and compositional properties of this novel material	888:956	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	10	7	theme	locomotor	1642:1650	arg1	recovery					1667:1674	locomotor or respiratory recovery	1642:1674	locomotor or respiratory recovery over an eight week period	1642:1700	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	8	8	theme	compressive	1260:1270	arg1	modulus					1272:1278	compressive modulus	1260:1278	compressive modulus	1260:1278	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	2	9	theme	fresh	238:242	arg1	grafts					275:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	9	10	theme	rat	1451:1453	arg1	model					1474:1478	a rat cervical contusion model	1449:1478	a rat cervical contusion model	1449:1478	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	5	11	theme	optimized	648:656	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	9	12	theme	contusion	1464:1472	arg1	model					1474:1478	a rat cervical contusion model	1449:1478	a rat cervical contusion model	1449:1478	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	2	13	from	recovery	341:348	arg1	models					373:378	experimental injury models	353:378	experimental injury models	353:378	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	7	14	theme	optimized	1085:1093	arg1	graft					1117:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	2	15	theme	nerve	269:273	arg1	grafts					275:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	5	16	theme	thermal	825:831	arg1	gelation					833:840	thermal gelation	825:840	thermal gelation	825:840	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	11	17	theme	inflammatory	2041:2052	arg1	milieu					2054:2059	the inflammatory milieu	2037:2059	the inflammatory milieu	2037:2059	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	11	18	theme	injectable	1894:1903	arg1	form					1905:1908	this novel injectable form	1883:1908	this novel injectable form of acellular nerve grafts	1883:1934	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	5	19	theme	decellularized	681:694	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	9	20	theme	iOA	1358:1360	arg1	solution					1362:1369	iOA solution	1358:1369	iOA solution	1358:1369	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	10	21	theme	iOA	1613:1615	arg1	treatment					1617:1625	iOA treatment	1613:1625	iOA treatment	1613:1625	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	7	22	theme	MAIN	1056:1059	arg1	RESULTS					1061:1067	MAIN RESULTS	1056:1067	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.	1056:1192	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	6	23	theme	novel	943:947	arg1	material					949:956	this novel material	938:956	this novel material	938:956	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	11	24	theme	axonal	2076:2081	arg1	growth					2083:2088	axonal growth	2076:2088	axonal growth	2076:2088	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	10	25	theme	distal	1754:1759	arg1	interface					1768:1776	the distal tissue interface	1750:1776	the distal tissue interface (p < 0.05)	1750:1787	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	10	25	theme	distal	1754:1759	arg1	p < 0.05					1779:1786	p < 0.05	1779:1786	p < 0.05	1779:1786	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	3	26	theme	spinal	433:438	arg1	contusion					440:448	a spinal contusion	431:448	a spinal contusion	431:448	Nonetheless, a scaffold that can be injected into a spinal contusion would be far less invasive to apply.
29380749	8	27	theme	central	1334:1340	arg1	tissue					1350:1355	rat central nervous tissue	1330:1355	rat central nervous tissue	1330:1355	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	10	28	theme	axonal	1810:1815	arg1	extension					1817:1825	enhanced axonal extension	1801:1825	enhanced axonal extension	1801:1825	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	9	29	theme	macrophages	1521:1531	arg1	ratio					1506:1510	the ratio	1502:1510	the ratio of M1:M2 macrophages	1502:1531	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	6	30	theme	time	1010:1013	arg1	points					1015:1020	acute and chronic time points	992:1020	acute and chronic time points following cervical contusion SCI	992:1053	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	0	31	theme	injured	71:77	arg1	cord					86:89	the injured spinal cord	67:89	the injured spinal cord	67:89	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	1	32	theme	quarter	137:143	arg1	individuals					153:163	a quarter million individuals	135:163	a quarter million individuals in the United States	135:184	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	5	33	theme	nerve	707:711	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	10	34	theme	respiratory	1655:1665	arg1	recovery					1667:1674	locomotor or respiratory recovery	1642:1674	locomotor or respiratory recovery over an eight week period	1642:1700	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	9	35	theme	regenerative	1558:1569	arg1	p < 0.05					1583:1590	p < 0.05	1583:1590	p < 0.05	1583:1590	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	9	35	theme	regenerative	1558:1569	arg1	phenotypes					1571:1580	regenerative phenotypes	1558:1580	regenerative phenotypes (p < 0.05)	1558:1591	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	2	36	theme	injury	366:371	arg1	models					373:378	experimental injury models	353:378	experimental injury models	353:378	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	0	37	theme	Injectable	0:9	arg1	hydrogels					11:19	Injectable hydrogels	0:19	Injectable hydrogels of optimized acellular nerve for injection	0:62	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	8	38	dep	properties	1245:1254	arg1	the					1229:1231	the	1229:1231	the	1229:1231	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	10	39	theme	eight	1684:1688	arg1	week					1690:1693	week	1690:1693	week	1690:1693	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	1	40	theme	Spinal	102:107	arg1	SCI					122:124	SCI	122:124	SCI	122:124	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	1	40	theme	Spinal	102:107	arg1	injury					114:119	OBJECTIVE Spinal cord injury	92:119	OBJECTIVE Spinal cord injury (SCI)	92:125	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	4	41	theme	injectable	517:526	arg1	graft					544:548	the first injectable acellular nerve graft	507:548	the first injectable acellular nerve graft for promoting repair after contusion SCI	507:589	We aimed to develop the first injectable acellular nerve graft for promoting repair after contusion SCI.
29380749	11	42	theme	acellular	1913:1921	arg1	grafts					1929:1934	acellular nerve grafts	1913:1934	acellular nerve grafts	1913:1934	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	1	43	theme	million	145:151	arg1	individuals					153:163	a quarter million individuals	135:163	a quarter million individuals in the United States	135:184	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	0	44	theme	acellular	34:42	arg1	nerve					44:48	optimized acellular nerve	24:48	optimized acellular nerve	24:48	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	11	45	theme	SIGNIFICANCE	1847:1858	arg1	data					1864:1867	SIGNIFICANCE Our data	1847:1867	SIGNIFICANCE Our data	1847:1867	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	9	46	theme	rat	1391:1393	arg1	cells					1403:1407	rat Schwann cells	1391:1407	rat Schwann cells in culture	1391:1418	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	11	47	theme	grafts	1929:1934	arg1	form					1905:1908	this novel injectable form	1883:1908	this novel injectable form of acellular nerve grafts	1883:1934	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	2	48	theme	axon	300:303	arg1	regeneration					305:316	spinal axon regeneration	293:316	spinal axon regeneration	293:316	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	7	49	dep	RESULTS	1061:1067	arg1	retains					1123:1129	retains	1123:1129	retains native chemical cues such as collagens and glycosaminoglycans	1123:1191	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	10	50	theme	coverage	1728:1735	arg1	percentage					1707:1716	the percentage	1703:1716	the percentage of axonal coverage	1703:1735	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	2	51	theme	functional	330:339	arg1	recovery					341:348	functional recovery	330:348	functional recovery in experimental injury models	330:378	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	1	52	theme	clinical	213:220	arg1	treatment					222:230	no clinical treatment	210:230	no clinical treatment	210:230	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	4	53	theme	nerve	538:542	arg1	graft					544:548	the first injectable acellular nerve graft	507:548	the first injectable acellular nerve graft for promoting repair after contusion SCI	507:589	We aimed to develop the first injectable acellular nerve graft for promoting repair after contusion SCI.
29380749	7	54	theme	nerve	1111:1115	arg1	graft					1117:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	2	55	theme	peripheral	258:267	arg1	grafts					275:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts	233:280	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	7	56	theme	iOA	1106:1108	arg1	graft					1117:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	5	57	theme	physiological	848:860	arg1	conditions					862:871	physiological conditions	848:871	physiological conditions	848:871	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	6	58	theme	multiple	888:895	arg1	properties					924:933	multiple physical and compositional properties	888:933	multiple physical and compositional properties of this novel material	888:956	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	7	59	theme	acellular	1095:1103	arg1	graft					1117:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	9	60	theme	cervical	1455:1462	arg1	model					1474:1478	a rat cervical contusion model	1449:1478	a rat cervical contusion model	1449:1478	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	5	61	dep	used	761:764	arg1	obtain					780:785	obtain	780:785	used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions	761:871	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	5	62	theme	OA	669:670	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	1	63	theme	United	172:177	arg1	States					179:184	the United States	168:184	the United States	168:184	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	8	64	theme	hydrogel	1205:1212	arg1	concentration					1214:1226	hydrogel concentration	1205:1226	hydrogel concentration	1205:1226	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	8	65	theme	iOA	1283:1285	arg1	modulus					1272:1278	compressive modulus	1260:1278	compressive modulus	1260:1278	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	8	65	theme	iOA	1283:1285	arg1	properties					1245:1254	rheological properties	1233:1254	rheological properties	1233:1254	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	5	66	theme	nerve-a	673:679	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	10	67	theme	enhanced	1801:1808	arg1	extension					1817:1825	enhanced axonal extension	1801:1825	enhanced axonal extension	1801:1825	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	1	68	theme	cord	109:112	arg1	SCI					122:124	SCI	122:124	SCI	122:124	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	1	68	theme	cord	109:112	arg1	injury					114:119	OBJECTIVE Spinal cord injury	92:119	OBJECTIVE Spinal cord injury (SCI)	92:125	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	8	69	theme	rheological	1233:1243	arg1	properties					1245:1254	rheological properties	1233:1254	rheological properties	1233:1254	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	6	70	theme	material	949:956	arg1	properties					924:933	multiple physical and compositional properties	888:933	multiple physical and compositional properties of this novel material	888:956	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	7	71	theme	injectable	1074:1083	arg1	graft					1117:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	This injectable optimized acellular (iOA) nerve graft	1069:1121	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	5	72	theme	acellular	658:666	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	9	73	theme	M1	1515:1516	arg1	macrophages					1521:1531	M1:M2 macrophages	1515:1531	M1:M2 macrophages	1515:1531	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	6	74	theme	cervical	1032:1039	arg1	contusion					1041:1049	cervical contusion	1032:1049	cervical contusion SCI	1032:1053	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	6	74	theme	cervical	1032:1039	arg1	SCI					1051:1053	SCI	1051:1053	SCI	1051:1053	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	9	75	theme	M2	1518:1519	arg1	macrophages					1521:1531	M1:M2 macrophages	1515:1531	M1:M2 macrophages	1515:1531	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	8	76	theme	rat	1330:1332	arg1	tissue					1350:1355	rat central nervous tissue	1330:1355	rat central nervous tissue	1330:1355	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	0	77	theme	spinal	79:84	arg1	cord					86:89	the injured spinal cord	67:89	the injured spinal cord	67:89	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	1	78	from	individuals	153:163	arg1	States					179:184	the United States	168:184	the United States	168:184	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	6	79	theme	chronic	1002:1008	arg1	points					1015:1020	acute and chronic time points	992:1020	acute and chronic time points following cervical contusion SCI	992:1053	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	8	80	theme	nervous	1342:1348	arg1	tissue					1350:1355	rat central nervous tissue	1330:1355	rat central nervous tissue	1330:1355	By varying hydrogel concentration, the rheological properties and compressive modulus of iOA were similar to that previous reported for rat central nervous tissue.
29380749	5	81	theme	peripheral	696:705	arg1	graft					713:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	optimized acellular (OA) nerve-a decellularized peripheral nerve graft	648:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	10	82	theme	tissue	1761:1766	arg1	interface					1768:1776	the distal tissue interface	1750:1776	the distal tissue interface (p < 0.05)	1750:1787	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	10	82	theme	tissue	1761:1766	arg1	p < 0.05					1779:1786	p < 0.05	1779:1786	p < 0.05	1779:1786	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	6	83	theme	acute	992:996	arg1	points					1015:1020	acute and chronic time points	992:1020	acute and chronic time points following cervical contusion SCI	992:1053	We quantified multiple physical and compositional properties of this novel material as well as tested its efficacy at acute and chronic time points following cervical contusion SCI.
29380749	10	84	theme	week	1690:1693	arg1	period					1695:1700	an eight week period	1681:1700	an eight week period	1681:1700	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
29380749	11	85	theme	novel	1888:1892	arg1	form					1905:1908	this novel injectable form	1883:1908	this novel injectable form of acellular nerve grafts	1883:1934	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	4	86	theme	first	511:515	arg1	graft					544:548	the first injectable acellular nerve graft	507:548	the first injectable acellular nerve graft for promoting repair after contusion SCI	507:589	We aimed to develop the first injectable acellular nerve graft for promoting repair after contusion SCI.
29380749	2	87	theme	experimental	353:364	arg1	models					373:378	experimental injury models	353:378	experimental injury models	353:378	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	0	88	theme	optimized	24:32	arg1	nerve					44:48	optimized acellular nerve	24:48	optimized acellular nerve	24:48	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	5	89	theme	injectable	790:799	arg1	solution					801:808	an injectable solution	787:808	an injectable solution that undergoes thermal gelation under physiological conditions	787:871	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	1	90	theme	OBJECTIVE	92:100	arg1	SCI					122:124	SCI	122:124	SCI	122:124	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	1	90	theme	OBJECTIVE	92:100	arg1	injury					114:119	OBJECTIVE Spinal cord injury	92:119	OBJECTIVE Spinal cord injury (SCI)	92:125	OBJECTIVE Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment.
29380749	4	91	theme	acellular	528:536	arg1	graft					544:548	the first injectable acellular nerve graft	507:548	the first injectable acellular nerve graft for promoting repair after contusion SCI	507:589	We aimed to develop the first injectable acellular nerve graft for promoting repair after contusion SCI.
29380749	0	92	theme	nerve	44:48	arg1	hydrogels					11:19	Injectable hydrogels	0:19	Injectable hydrogels of optimized acellular nerve for injection	0:62	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	5	93	used	used	761:764	arg2	APPROACH					592:599	APPROACH	592:599	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft	592:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	11	94	theme	nerve	1923:1927	arg1	grafts					1929:1934	acellular nerve grafts	1913:1934	acellular nerve grafts	1913:1934	SIGNIFICANCE Our data indicate that this novel injectable form of acellular nerve grafts is amenable for use after contusion SCI and may bolster a simultaneous therapy by acutely modulating the inflammatory milieu and supporting axonal growth.
29380749	7	95	theme	chemical	1138:1145	arg1	glycosaminoglycans					1174:1191	glycosaminoglycans	1174:1191	glycosaminoglycans	1174:1191	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	7	95	theme	chemical	1138:1145	arg1	cues					1147:1150	native chemical cues	1131:1150	native chemical cues such as collagens and glycosaminoglycans	1131:1191	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	7	95	theme	chemical	1138:1145	arg1	collagens					1160:1168	collagens	1160:1168	collagens	1160:1168	MAIN RESULTS This injectable optimized acellular (iOA) nerve graft retains native chemical cues such as collagens and glycosaminoglycans.
29380749	5	96	dep	APPROACH	592:599	arg1	report					604:609	report	604:609	report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft	604:717	APPROACH We report a method to enzymatically solubilize optimized acellular (OA) nerve-a decellularized peripheral nerve graft developed in our laboratory and currently used clinically-to obtain an injectable solution that undergoes thermal gelation under physiological conditions.
29380749	9	97	theme	Schwann	1395:1401	arg1	cells					1403:1407	rat Schwann cells	1391:1407	rat Schwann cells in culture	1391:1418	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	9	98	from	cells	1403:1407	arg1	culture					1412:1418	culture	1412:1418	culture	1412:1418	iOA solution was compatible with rat Schwann cells in culture, and hydrogel injection into a rat cervical contusion model significantly reduced the ratio of M1:M2 macrophages after one week, favoring regenerative phenotypes (p < 0.05).
29380749	2	99	theme	spinal	293:298	arg1	regeneration					305:316	spinal axon regeneration	293:316	spinal axon regeneration	293:316	Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models.
29380749	0	100	dep	hydrogels	11:19	arg1	cord					86:89	the injured spinal cord	67:89	the injured spinal cord	67:89	Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord.
29380749	10	101	theme	axonal	1721:1726	arg1	coverage					1728:1735	axonal coverage	1721:1735	axonal coverage	1721:1735	Furthermore, while iOA treatment did not affect locomotor or respiratory recovery over an eight week period, the percentage of axonal coverage increased at the distal tissue interface (p < 0.05), suggesting enhanced axonal extension within this region.
30556771	0	0	theme	composite	79:87	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	0	theme	composite	79:87	arg1	hydrogel					89:96	hyaluronic acid composite hydrogel	63:96	hyaluronic acid composite hydrogel as wound dressing	63:114	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	1	1	theme	hyaluronic	131:140	arg1	nanocrystals					166:177	cellulose nanocrystals	156:177	cellulose nanocrystals (CNC)	156:183	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	1	theme	hyaluronic	131:140	arg1	materials					199:207	promising materials	189:207	promising materials for skin wound care	189:227	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	1	theme	hyaluronic	131:140	arg1	HA					148:149	HA	148:149	HA	148:149	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	1	theme	hyaluronic	131:140	arg1	Gelatin					117:123	Gelatin	117:123	Gelatin (GA)	117:128	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	1	theme	hyaluronic	131:140	arg1	acid					142:145	hyaluronic acid	131:145	hyaluronic acid (HA)	131:150	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	6	2	theme	different	591:599	arg1	days					601:604	different days	591:604	different days	591:604	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	0	3	theme	acid	74:77	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	3	theme	acid	74:77	arg1	hydrogel					89:96	hyaluronic acid composite hydrogel	63:96	hyaluronic acid composite hydrogel as wound dressing	63:114	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	9	4	theme	hydrogels	1000:1008	arg1	property					984:991	the property	980:991	the property of the hydrogels	980:1008	CNC significantly enhanced the property of the hydrogels and play a vital role according to the rheology and swelling results.
30556771	1	5	theme	skin	213:216	arg1	care					224:227	skin wound care	213:227	skin wound care	213:227	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	6	theme	wound	218:222	arg1	care					224:227	skin wound care	213:227	skin wound care	213:227	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	6	7	theme	staining	722:729	arg1	kits					731:734	staining kits	722:734	staining kits	722:734	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	6	8	theme	CCK-8	670:674	arg1	methods					676:682	CCK-8 methods	670:682	CCK-8 methods	670:682	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	6	9	theme	prepared	648:655	arg1	hydrogels					657:665	the prepared hydrogels	644:665	the prepared hydrogels	644:665	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	11	10	theme	natural	1223:1229	arg1	hydrogel					1241:1248	the natural GA-HA-CNC hydrogel	1219:1248	the natural GA-HA-CNC hydrogel	1219:1248	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	8	11	theme	pore	922:925	arg1	diameter					927:934	the pore diameter	918:934	the pore diameter about 80-120 µm	918:950	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	7	12	theme	amide	812:816	arg1	bond					818:821	the amide bond	808:821	the amide bond	808:821	FTIR revealed the combination between GA, HA and CNC was attributed to the amide bond and hydrogen bonding.
30556771	0	13	theme	wound	101:105	arg1	dressing					107:114	wound dressing	101:114	wound dressing	101:114	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	11	14	theme	skin	1278:1281	arg1	repair					1289:1294	the skin wound repair	1274:1294	the skin wound repair	1274:1294	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	10	15	theme	NIH-3T3	1118:1124	arg1	cells					1126:1130	NIH-3T3 cells	1118:1130	NIH-3T3 cells	1118:1130	The cells culture results showed that NIH-3T3 cells can attached to, grow, and proliferate well on the GA-HA-CNC hydrogels.
30556771	6	16	theme	hydrogels	657:665	arg1	cytotoxicity					628:639	the cytotoxicity	624:639	the cytotoxicity of the prepared hydrogels	624:665	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	5	17	from	results	497:503	arg1	property					471:478	the physical property	458:478	the physical property from rheological results and swelling ratio	458:522	We accessed the physical property from rheological results and swelling ratio.
30556771	11	18	theme	great	1254:1258	arg1	potential					1260:1268	great potential	1254:1268	great potential for the skin wound repair	1254:1294	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	5	19	theme	physical	462:469	arg1	property					471:478	the physical property	458:478	the physical property from rheological results and swelling ratio	458:522	We accessed the physical property from rheological results and swelling ratio.
30556771	3	20	theme	GA-HA-CNC	351:359	arg1	hydrogels					361:369	GA-HA-CNC hydrogels	351:369	GA-HA-CNC hydrogels	351:369	The composition and mechanism of GA-HA-CNC hydrogels were confirmed by FTIR.
30556771	10	21	theme	GA-HA-CNC	1183:1191	arg1	hydrogels					1193:1201	the GA-HA-CNC hydrogels	1179:1201	the GA-HA-CNC hydrogels	1179:1201	The cells culture results showed that NIH-3T3 cells can attached to, grow, and proliferate well on the GA-HA-CNC hydrogels.
30556771	8	22	theme	SEM	845:847	arg1	results					849:855	SEM results	845:855	SEM results	845:855	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	2	23	theme	GA-HA-CNC	248:256	arg1	hydrogels					258:266	the GA-HA-CNC hydrogels	244:266	the GA-HA-CNC hydrogels	244:266	In this study the GA-HA-CNC hydrogels were prepared by cross-linking and freeze-drying.
30556771	5	24	from	ratio	518:522	arg1	property					471:478	the physical property	458:478	the physical property from rheological results and swelling ratio	458:522	We accessed the physical property from rheological results and swelling ratio.
30556771	6	25	theme	live/dead	688:696	arg1	diagram					708:714	live/dead pictorial diagram	688:714	live/dead pictorial diagram	688:714	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	4	26	theme	pore	414:417	arg1	size					419:422	pore size	414:422	pore size	414:422	The morphology and pore size were obtained by SEM.
30556771	0	27	theme	nanocrystals	40:51	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	27	theme	nanocrystals	40:51	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	27	theme	nanocrystals	40:51	arg1	hydrogel					89:96	hyaluronic acid composite hydrogel	63:96	hyaluronic acid composite hydrogel as wound dressing	63:114	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	27	theme	nanocrystals	40:51	arg1	gelatin					54:60	gelatin	54:60	gelatin	54:60	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	9	28	theme	rheology	1049:1056	arg1	results					1071:1077	the rheology and swelling results	1045:1077	the rheology and swelling results	1045:1077	CNC significantly enhanced the property of the hydrogels and play a vital role according to the rheology and swelling results.
30556771	0	29	theme	cellulose	30:38	arg1	nanocrystals					40:51	cellulose nanocrystals	30:51	cellulose nanocrystals	30:51	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	8	30	with	spongy	905:910	arg1	diameter					927:934	the pore diameter	918:934	the pore diameter about 80-120 µm	918:950	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	3	31	theme	hydrogels	361:369	arg1	mechanism					338:346	mechanism	338:346	mechanism	338:346	The composition and mechanism of GA-HA-CNC hydrogels were confirmed by FTIR.
30556771	3	31	theme	hydrogels	361:369	arg1	composition					322:332	composition	322:332	composition	322:332	The composition and mechanism of GA-HA-CNC hydrogels were confirmed by FTIR.
30556771	10	32	theme	culture	1090:1096	arg1	results					1098:1104	The cells culture results	1080:1104	The cells culture results	1080:1104	The cells culture results showed that NIH-3T3 cells can attached to, grow, and proliferate well on the GA-HA-CNC hydrogels.
30556771	5	33	theme	swelling	509:516	arg1	ratio					518:522	swelling ratio	509:522	swelling ratio	509:522	We accessed the physical property from rheological results and swelling ratio.
30556771	6	34	theme	NIH-3T3	525:531	arg1	cells					533:537	NIH-3T3 cells	525:537	NIH-3T3 cells	525:537	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	10	35	theme	cells	1084:1088	arg1	results					1098:1104	The cells culture results	1080:1104	The cells culture results	1080:1104	The cells culture results showed that NIH-3T3 cells can attached to, grow, and proliferate well on the GA-HA-CNC hydrogels.
30556771	11	36	theme	GA-HA-CNC	1231:1239	arg1	hydrogel					1241:1248	the natural GA-HA-CNC hydrogel	1219:1248	the natural GA-HA-CNC hydrogel	1219:1248	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	8	37	theme	GA-HA-CNC	880:888	arg1	spongy					905:910	spongy	905:910	spongy	905:910	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	8	37	theme	GA-HA-CNC	880:888	arg1	hydrogels					890:898	the drying GA-HA-CNC hydrogels	869:898	the drying GA-HA-CNC hydrogels	869:898	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	11	38	contain	has	1250:1252	arg1	hydrogel					1241:1248	the natural GA-HA-CNC hydrogel	1219:1248	the natural GA-HA-CNC hydrogel	1219:1248	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	11	38	contain	has	1250:1252	arg2	potential					1260:1268	great potential	1254:1268	great potential for the skin wound repair	1254:1294	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	8	39	theme	drying	873:878	arg1	spongy					905:910	spongy	905:910	spongy	905:910	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	8	39	theme	drying	873:878	arg1	hydrogels					890:898	the drying GA-HA-CNC hydrogels	869:898	the drying GA-HA-CNC hydrogels	869:898	SEM results showed that the drying GA-HA-CNC hydrogels were spongy, with the pore diameter about 80-120 µm.
30556771	9	40	theme	swelling	1062:1069	arg1	results					1071:1077	the rheology and swelling results	1045:1077	the rheology and swelling results	1045:1077	CNC significantly enhanced the property of the hydrogels and play a vital role according to the rheology and swelling results.
30556771	3	41	dep	composition	322:332	arg1	The					318:320	The	318:320	The	318:320	The composition and mechanism of GA-HA-CNC hydrogels were confirmed by FTIR.
30556771	6	42	theme	pictorial	698:706	arg1	diagram					708:714	live/dead pictorial diagram	688:714	live/dead pictorial diagram	688:714	NIH-3T3 cells were inoculated into the hydrogels and cultured for different days, then we analyzed the cytotoxicity of the prepared hydrogels by CCK-8 methods and live/dead pictorial diagram using staining kits.
30556771	9	43	theme	vital	1021:1025	arg1	role					1027:1030	a vital role	1019:1030	a vital role	1019:1030	CNC significantly enhanced the property of the hydrogels and play a vital role according to the rheology and swelling results.
30556771	0	44	theme	hyaluronic	63:72	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	0	44	theme	hyaluronic	63:72	arg1	hydrogel					89:96	hyaluronic acid composite hydrogel	63:96	hyaluronic acid composite hydrogel as wound dressing	63:114	Preparation and properties of cellulose nanocrystals, gelatin, hyaluronic acid composite hydrogel as wound dressing.
30556771	1	45	theme	cellulose	156:164	arg1	nanocrystals					166:177	cellulose nanocrystals	156:177	cellulose nanocrystals (CNC)	156:183	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	45	theme	cellulose	156:164	arg1	materials					199:207	promising materials	189:207	promising materials for skin wound care	189:227	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	45	theme	cellulose	156:164	arg1	acid					142:145	hyaluronic acid	131:145	hyaluronic acid (HA)	131:150	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	45	theme	cellulose	156:164	arg1	Gelatin					117:123	Gelatin	117:123	Gelatin (GA)	117:128	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	45	theme	cellulose	156:164	arg1	CNC					180:182	CNC	180:182	CNC	180:182	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	46	theme	promising	189:197	arg1	nanocrystals					166:177	cellulose nanocrystals	156:177	cellulose nanocrystals (CNC)	156:183	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	46	theme	promising	189:197	arg1	materials					199:207	promising materials	189:207	promising materials for skin wound care	189:227	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	46	theme	promising	189:197	arg1	Gelatin					117:123	Gelatin	117:123	Gelatin (GA)	117:128	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	1	46	theme	promising	189:197	arg1	acid					142:145	hyaluronic acid	131:145	hyaluronic acid (HA)	131:150	Gelatin (GA), hyaluronic acid (HA) and cellulose nanocrystals (CNC) are promising materials for skin wound care.
30556771	11	47	theme	wound	1283:1287	arg1	repair					1289:1294	the skin wound repair	1274:1294	the skin wound repair	1274:1294	In conclusion, the natural GA-HA-CNC hydrogel has great potential for the skin wound repair.
30556771	5	48	theme	rheological	485:495	arg1	results					497:503	rheological results	485:503	rheological results	485:503	We accessed the physical property from rheological results and swelling ratio.
30556771	7	49	theme	hydrogen	827:834	arg1	bonding					836:842	hydrogen bonding	827:842	hydrogen bonding	827:842	FTIR revealed the combination between GA, HA and CNC was attributed to the amide bond and hydrogen bonding.
31313242	6	0	theme	nutrients	1155:1163	arg1	encapsulation					1165:1177	nutrients encapsulation	1155:1177	nutrients encapsulation in the food industry	1155:1198	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	5	1	theme	molecular	1018:1026	arg1	bonds					1028:1032	molecular bonds	1018:1032	molecular bonds	1018:1032	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	1	2	theme	simulated	267:275	arg1	juice					285:289	simulated gastric juice	267:289	simulated gastric juice	267:289	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	4	3	dep	%	810:810	arg1	v/v					813:815	v/v	813:815	v/v	813:815	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	6	4	theme	food	1186:1189	arg1	industry					1191:1198	the food industry	1182:1198	the food industry	1182:1198	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	3	5	theme	mean	523:526	arg1	MD					538:539	MD	538:539	MD	538:539	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	5	theme	mean	523:526	arg1	diameter					528:535	mean diameter	523:535	mean diameter (MD) of the microcapsules	523:561	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	1	6	theme	gastric	277:283	arg1	juice					285:289	simulated gastric juice	267:289	simulated gastric juice	267:289	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	0	7	theme	Bacteria	112:119	arg1	Behavior					90:97	Release Behavior	82:97	Release Behavior	82:97	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	0	7	theme	Bacteria	112:119	arg1	Survivability					64:76	Survivability	64:76	Survivability	64:76	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	3	8	theme	viable	670:675	arg1	cells					677:681	viable cells	670:681	viable cells	670:681	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	4	9	theme	10	808:809	arg1	%					810:810	%	810:810	%	810:810	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	4	10	theme	Optimal	742:748	arg1	composition					750:760	Optimal composition	742:760	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase	742:848	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	6	11	from	encapsulation	1165:1177	arg1	industry					1191:1198	the food industry	1182:1198	the food industry	1182:1198	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	0	12	theme	Probiotic	102:110	arg1	Bacteria					112:119	Probiotic Bacteria	102:119	Probiotic Bacteria	102:119	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	5	13	theme	surface	998:1004	arg1	morphology					1006:1015	surface morphology	998:1015	surface morphology	998:1015	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	14	theme	Physicochemical	880:894	arg1	characteristics					896:910	Physicochemical characteristics	880:910	Physicochemical characteristics of the optimized matrix	880:934	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	4	15	theme	%	791:791	arg1	WPI					799:801	10% (w/v) WPI	789:801	10% (w/v) WPI	789:801	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	1	16	theme	acidophilus	181:191	arg1	survivability					161:173	the survivability	157:173	the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ)	157:332	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	3	17	theme	Box-Behnken	709:719	arg1	design					734:739	Box-Behnken experimental design	709:739	Box-Behnken experimental design	709:739	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	4	18	theme	%	810:810	arg1	gum					825:827	10% (v/v) AL-WPI gum	808:827	10% (v/v) AL-WPI gum	808:827	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	3	19	theme	WPI	447:449	arg1	compositions					424:435	Optimal compositions	416:435	Optimal compositions of AL and WPI and their ratio in the aqueous phase	416:486	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	20	theme	Optimal	416:422	arg1	compositions					424:435	Optimal compositions	416:435	Optimal compositions of AL and WPI and their ratio in the aqueous phase	416:486	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	21	theme	experimental	721:732	arg1	design					734:739	Box-Behnken experimental design	709:739	Box-Behnken experimental design	709:739	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	22	theme	encapsulation	578:590	arg1	EE					604:605	EE	604:605	EE	604:605	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	22	theme	encapsulation	578:590	arg1	efficiency					592:601	encapsulation efficiency	578:601	encapsulation efficiency (EE)	578:606	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	2	23	theme	gelation	396:403	arg1	technique					405:413	emulsification/internal gelation technique	372:413	emulsification/internal gelation technique	372:413	Microcapsules were prepared based on emulsification/internal gelation technique.
31313242	0	24	theme	Protein	30:36	arg1	Isolate					38:44	Alginate-Whey Protein Isolate	16:44	Alginate-Whey Protein Isolate	16:44	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	3	25	theme	microcapsules	549:561	arg1	MD					538:539	MD	538:539	MD	538:539	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	25	theme	microcapsules	549:561	arg1	diameter					528:535	mean diameter	523:535	mean diameter (MD) of the microcapsules	523:561	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	6	26	theme	good	1138:1141	arg1	promise					1143:1149	a good promise	1136:1149	a good promise for nutrients encapsulation in the food industry	1136:1198	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	6	26	theme	good	1138:1141	arg1	biocomposite					1120:1131	the proposed biocomposite	1107:1131	the proposed biocomposite	1107:1131	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	1	27	theme	alginate-whey	209:221	arg1	biocomposite					248:259	alginate-whey protein isolate (AL-WPI) biocomposite	209:259	alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ)	209:295	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	27	theme	alginate-whey	209:221	arg1	SGJ					292:294	SGJ	292:294	SGJ	292:294	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	0	28	theme	Alginate-Whey	16:28	arg1	Isolate					38:44	Alginate-Whey Protein Isolate	16:44	Alginate-Whey Protein Isolate	16:44	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	4	29	theme	AL-WPI	818:823	arg1	gum					825:827	10% (v/v) AL-WPI gum	808:827	10% (v/v) AL-WPI gum	808:827	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	5	30	theme	XRD	972:974	arg1	analysis					976:983	SEM, FTIR, and XRD analysis	957:983	SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid	957:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	1	31	theme	protein	223:229	arg1	biocomposite					248:259	alginate-whey protein isolate (AL-WPI) biocomposite	209:259	alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ)	209:295	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	31	theme	protein	223:229	arg1	SGJ					292:294	SGJ	292:294	SGJ	292:294	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	3	32	theme	AL	440:441	arg1	compositions					424:435	Optimal compositions	416:435	Optimal compositions of AL and WPI and their ratio in the aqueous phase	416:486	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	1	33	theme	simulated	301:309	arg1	juice					322:326	simulated intestinal juice	301:326	simulated intestinal juice (SIJ)	301:332	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	33	theme	simulated	301:309	arg1	SIJ					329:331	SIJ	329:331	SIJ	329:331	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	5	34	theme	matrix	929:934	arg1	characteristics					896:910	Physicochemical characteristics	880:910	Physicochemical characteristics of the optimized matrix	880:934	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	1	35	theme	isolate	231:237	arg1	biocomposite					248:259	alginate-whey protein isolate (AL-WPI) biocomposite	209:259	alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ)	209:295	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	35	theme	isolate	231:237	arg1	SGJ					292:294	SGJ	292:294	SGJ	292:294	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	0	36	theme	Isolate	38:44	arg1	Optimization					0:11	Optimization	0:11	Optimization of Alginate-Whey Protein Isolate	0:44	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	1	37	theme	intestinal	311:320	arg1	juice					322:326	simulated intestinal juice	301:326	simulated intestinal juice (SIJ)	301:332	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	37	theme	intestinal	311:320	arg1	SIJ					329:331	SIJ	329:331	SIJ	329:331	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	3	38	theme	SGJ	638:640	arg1	Viability					643:651	SGJ (Viability)	638:652	SGJ (Viability)	638:652	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	4	39	theme	10	789:790	arg1	%					791:791	%	791:791	%	791:791	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	1	40	theme	present	126:132	arg1	study					134:138	The present study	122:138	The present study	122:138	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	5	41	theme	crystalline	1039:1049	arg1	nature					1051:1056	crystalline nature	1039:1056	crystalline nature of such hydrocolloid	1039:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	42	theme	such	1061:1064	arg1	hydrocolloid					1066:1077	such hydrocolloid	1061:1077	such hydrocolloid	1061:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	3	43	from	compositions	424:435	arg1	phase					482:486	the aqueous phase	470:486	the aqueous phase	470:486	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	5	44	theme	SEM	957:959	arg1	analysis					976:983	SEM, FTIR, and XRD analysis	957:983	SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid	957:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	45	theme	hydrocolloid	1066:1077	arg1	nature					1051:1056	crystalline nature	1039:1056	crystalline nature of such hydrocolloid	1039:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	45	theme	hydrocolloid	1066:1077	arg1	morphology					1006:1015	surface morphology	998:1015	surface morphology	998:1015	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	45	theme	hydrocolloid	1066:1077	arg1	bonds					1028:1032	molecular bonds	1018:1032	molecular bonds	1018:1032	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	5	46	theme	FTIR	962:965	arg1	analysis					976:983	SEM, FTIR, and XRD analysis	957:983	SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid	957:1077	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	6	47	theme	proposed	1111:1118	arg1	promise					1143:1149	a good promise	1136:1149	a good promise for nutrients encapsulation in the food industry	1136:1198	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	6	47	theme	proposed	1111:1118	arg1	biocomposite					1120:1131	the proposed biocomposite	1107:1131	the proposed biocomposite	1107:1131	It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry.
31313242	1	48	theme	AL-WPI	240:245	arg1	biocomposite					248:259	alginate-whey protein isolate (AL-WPI) biocomposite	209:259	alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ)	209:295	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	1	48	theme	AL-WPI	240:245	arg1	SGJ					292:294	SGJ	292:294	SGJ	292:294	The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ).
31313242	3	49	theme	cells	626:630	arg1	EE					604:605	EE	604:605	EE	604:605	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	49	theme	cells	626:630	arg1	survivability					609:621	survivability	609:621	survivability of cells under SGJ (Viability)	609:652	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	49	theme	cells	626:630	arg1	efficiency					592:601	encapsulation efficiency	578:601	encapsulation efficiency (EE)	578:606	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	49	theme	cells	626:630	arg1	release					659:665	release	659:665	release of viable cells under SIJ (Release) using Box-Behnken experimental design	659:739	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	0	50	theme	Release	82:88	arg1	Behavior					90:97	Release Behavior	82:97	Release Behavior	82:97	Optimization of Alginate-Whey Protein Isolate Microcapsules for Survivability and Release Behavior of Probiotic Bacteria.
31313242	5	51	theme	optimized	919:927	arg1	matrix					929:934	the optimized matrix	915:934	the optimized matrix	915:934	Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid.
31313242	3	52	theme	aqueous	474:480	arg1	phase					482:486	the aqueous phase	470:486	the aqueous phase	470:486	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	2	53	theme	emulsification/internal	372:394	arg1	technique					405:413	emulsification/internal gelation technique	372:413	emulsification/internal gelation technique	372:413	Microcapsules were prepared based on emulsification/internal gelation technique.
31313242	3	54	theme	cells	677:681	arg1	EE					604:605	EE	604:605	EE	604:605	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	54	theme	cells	677:681	arg1	survivability					609:621	survivability	609:621	survivability of cells under SGJ (Viability)	609:652	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	54	theme	cells	677:681	arg1	efficiency					592:601	encapsulation efficiency	578:601	encapsulation efficiency (EE)	578:606	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	3	54	theme	cells	677:681	arg1	release					659:665	release	659:665	release of viable cells under SIJ (Release) using Box-Behnken experimental design	659:739	Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design.
31313242	4	55	dep	%	791:791	arg1	w/v					794:796	w/v	794:796	w/v	794:796	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
31313242	4	56	theme	aqueous	836:842	arg1	phase					844:848	the aqueous phase	832:848	the aqueous phase	832:848	Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically.
30214024	0	0	theme	birth	81:85	arg1	effects					60:66	the effects	56:66	the effects of caesarean birth on infant gut microbiota	56:110	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	6	1	theme	microbiota	859:868	arg1	composition					870:880	microbiota composition	859:880	microbiota composition	859:880	In the vaginally born infants, maternal secretor status was not associated with microbiota composition.
30214024	10	2	theme	microbiota	1467:1476	arg1	development					1478:1488	compromised microbiota development	1455:1488	compromised microbiota development	1455:1488	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	6	3	theme	secretor	819:826	arg1	status					828:833	maternal secretor status	810:833	maternal secretor status	810:833	In the vaginally born infants, maternal secretor status was not associated with microbiota composition.
30214024	1	4	theme	components	138:147	arg1	components					138:147	the most abundant components	120:147	the most abundant components in human milk	120:161	One of the most abundant components in human milk is formed by oligosaccharides, which are poorly digested by the infant.
30214024	1	4	theme	components	138:147	arg1	One					113:115	One	113:115	One	113:115	One of the most abundant components in human milk is formed by oligosaccharides, which are poorly digested by the infant.
30214024	0	5	theme	caesarean	71:79	arg1	birth					81:85	caesarean birth	71:85	caesarean birth	71:85	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	0	6	from	oligosaccharides	12:27	arg1	milk					41:44	mother's milk	32:44	mother's milk	32:44	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	10	7	theme	compromised	1455:1465	arg1	development					1478:1488	compromised microbiota development	1455:1488	compromised microbiota development	1455:1488	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	1	8	from	components	138:147	arg1	milk					158:161	human milk	152:161	human milk	152:161	One of the most abundant components in human milk is formed by oligosaccharides, which are poorly digested by the infant.
30214024	6	9	theme	born	796:799	arg1	infants					801:807	the vaginally born infants	782:807	the vaginally born infants	782:807	In the vaginally born infants, maternal secretor status was not associated with microbiota composition.
30214024	0	10	theme	infant	90:95	arg1	microbiota					101:110	infant gut microbiota	90:110	infant gut microbiota	90:110	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	2	11	theme	breast	270:275	arg1	milk					277:280	breast milk	270:280	breast milk	270:280	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	10	12	theme	secretor	1391:1398	arg1	status					1400:1405	maternal secretor status	1382:1405	maternal secretor status	1382:1405	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	2	13	theme	maternal	326:333	arg1	genotype					351:358	maternal secretor (FUT2) genotype	326:358	maternal secretor (FUT2) genotype	326:358	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	7	14	from	caesarean-born	890:903	arg1	pronounced					978:987	pronounced	978:987	pronounced	978:987	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	5	15	theme	N = 15	730:735	arg1	mothers					738:744	secretor (N = 76) and non-secretor (N = 15) mothers	694:744	mothers	738:744	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	7	16	theme	secretor	1052:1059	arg1	mothers					1061:1067	secretor mothers	1052:1067	secretor mothers	1052:1067	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	10	17	theme	maternal	1382:1389	arg1	status					1400:1405	maternal secretor status	1382:1405	maternal secretor status	1382:1405	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	8	18	theme	non-secretor	1183:1194	arg1	mothers					1196:1202	non-secretor mothers	1183:1202	non-secretor mothers	1183:1202	Particularly bifidobacteria were strongly depleted and enterococci increased among the caesarean-born infants of non-secretor mothers.
30214024	1	19	theme	human	152:156	arg1	milk					158:161	human milk	152:161	human milk	152:161	One of the most abundant components in human milk is formed by oligosaccharides, which are poorly digested by the infant.
30214024	0	20	theme	gut	97:99	arg1	microbiota					101:110	infant gut microbiota	90:110	infant gut microbiota	90:110	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	10	21	from	infants	1442:1448	arg1	influential					1427:1437	influential	1427:1437	influential	1427:1437	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	2	22	theme	oligosaccharide	239:253	arg1	dependent					313:321	dependent	313:321	dependent	313:321	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	2	22	theme	oligosaccharide	239:253	arg1	composition					255:265	The oligosaccharide composition	235:265	The oligosaccharide composition of breast milk	235:280	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	3	23	theme	human	419:423	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	3	24	theme	mothers	493:499	arg1	milk					472:475	the milk	468:475	the milk of non-secretor mothers	468:499	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	3	25	from	absent	458:463	arg1	milk					472:475	the milk	468:475	the milk of non-secretor mothers	468:499	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	3	26	theme	containing	391:400	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	0	27	theme	Fucosylated	0:10	arg1	oligosaccharides					12:27	Fucosylated oligosaccharides	0:27	Fucosylated oligosaccharides in mother's milk	0:44	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	9	28	theme	secretor	1275:1282	arg1	mothers					1284:1290	secretor mothers	1275:1290	secretor mothers	1275:1290	Furthermore, Akkermansia was increased in the section-born infants of secretor mothers, supporting the suggestion that this organism may degrade HMOs.
30214024	5	29	theme	account	759:765	arg1	mode					773:776	account birth mode	759:776	account birth mode	759:776	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	3	30	theme	α1-2	402:405	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	5	31	theme	birth	767:771	arg1	mode					773:776	account birth mode	759:776	account birth mode	759:776	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	4	32	theme	bacteria	521:528	arg1	strains					510:516	Several strains	502:516	Several strains of bacteria in the infant gut	502:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	5	33	theme	gut	659:661	arg1	composition					674:684	infant gut microbiota composition	652:684	infant gut microbiota composition	652:684	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	3	34	from	milk	472:475	arg1	absent					458:463	absent	458:463	absent	458:463	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	4	35	from	bacteria	521:528	arg1	gut					544:546	the infant gut	533:546	the infant gut	533:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	4	36	theme	infant	537:542	arg1	gut					544:546	the infant gut	533:546	the infant gut	533:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	5	37	theme	microbiota	663:672	arg1	composition					674:684	infant gut microbiota composition	652:684	infant gut microbiota composition	652:684	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	6	38	theme	maternal	810:817	arg1	status					828:833	maternal secretor status	810:833	maternal secretor status	810:833	In the vaginally born infants, maternal secretor status was not associated with microbiota composition.
30214024	2	39	theme	milk	277:280	arg1	dependent					313:321	dependent	313:321	dependent	313:321	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	2	39	theme	milk	277:280	arg1	composition					255:265	The oligosaccharide composition	235:265	The oligosaccharide composition of breast milk	235:280	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	10	40	with	infants	1442:1448	arg1	development					1478:1488	compromised microbiota development	1455:1488	compromised microbiota development	1455:1488	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	7	41	theme	non-secretor	1010:1021	arg1	mothers					1023:1029	non-secretor mothers	1010:1029	non-secretor mothers	1010:1029	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	2	42	theme	FUT2	345:348	arg1	genotype					351:358	maternal secretor (FUT2) genotype	326:358	maternal secretor (FUT2) genotype	326:358	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	3	43	theme	milk	425:428	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	3	44	theme	milk	386:389	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	4	45	theme	Several	502:508	arg1	strains					510:516	Several strains	502:516	Several strains of bacteria in the infant gut	502:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	4	46	from	strains	510:516	arg1	gut					544:546	the infant gut	533:546	the infant gut	533:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	8	47	theme	mothers	1196:1202	arg1	infants					1172:1178	the caesarean-born infants	1153:1178	the caesarean-born infants of non-secretor mothers	1153:1202	Particularly bifidobacteria were strongly depleted and enterococci increased among the caesarean-born infants of non-secretor mothers.
30214024	4	48	from	gut	544:546	arg1	strains					510:516	Several strains	502:516	Several strains of bacteria in the infant gut	502:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	3	49	theme	Secretor	361:368	arg1	mothers					370:376	Secretor mothers	361:376	Secretor mothers	361:376	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	5	50	theme	secretor	694:701	arg1	N = 76					704:709	secretor (N = 76) and non-secretor (N = 15) mothers	694:744	N = 76	704:709	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	0	51	from	effects	60:66	arg1	microbiota					101:110	infant gut microbiota	90:110	infant gut microbiota	90:110	Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.
30214024	7	52	theme	mothers	1023:1029	arg1	infants					999:1005	the infants	995:1005	the infants of non-secretor mothers	995:1029	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	9	53	theme	section-born	1251:1262	arg1	infants					1264:1270	the section-born infants	1247:1270	the section-born infants of secretor mothers	1247:1290	Furthermore, Akkermansia was increased in the section-born infants of secretor mothers, supporting the suggestion that this organism may degrade HMOs.
30214024	4	54	contain	have	548:551	arg1	strains					510:516	Several strains	502:516	Several strains of bacteria in the infant gut	502:546	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	4	54	contain	have	548:551	arg2	capacity					557:564	the capacity to utilise human milk oligosaccharides (HMOs)	553:610	the capacity to utilise human milk oligosaccharides (HMOs)	553:610	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	7	55	theme	patterns	959:966	arg1	patterns					959:966	the caesarean-associated microbiota patterns	923:966	the caesarean-associated microbiota patterns	923:966	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	7	55	theme	patterns	959:966	arg1	many					915:918	many	915:918	many	915:918	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	3	56	theme	fucosylated	407:417	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	10	57	theme	corrective	1533:1542	arg1	supplementation					1544:1558	corrective supplementation	1533:1558	corrective supplementation	1533:1558	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	5	58	theme	infant	652:657	arg1	composition					674:684	infant gut microbiota composition	652:684	infant gut microbiota composition	652:684	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	8	59	theme	caesarean-born	1157:1170	arg1	infants					1172:1178	the caesarean-born infants	1153:1178	the caesarean-born infants of non-secretor mothers	1153:1202	Particularly bifidobacteria were strongly depleted and enterococci increased among the caesarean-born infants of non-secretor mothers.
30214024	3	60	gly	fucosylated	407:417	arg1	oligosaccharides					430:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	milk containing α1-2 fucosylated human milk oligosaccharides	386:445	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	7	61	theme	caesarean-associated	927:946	arg1	patterns					959:966	the caesarean-associated microbiota patterns	923:966	the caesarean-associated microbiota patterns	923:966	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	1	62	theme	abundant	129:136	arg1	components					138:147	the most abundant components	120:147	the most abundant components in human milk	120:161	One of the most abundant components in human milk is formed by oligosaccharides, which are poorly digested by the infant.
30214024	7	63	from	pronounced	978:987	arg1	caesarean-born					890:903	the caesarean-born	886:903	the caesarean-born	886:903	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	10	64	from	influential	1427:1437	arg1	infants					1442:1448	infants	1442:1448	infants with compromised microbiota development	1442:1488	The results indicate that maternal secretor status may be particularly influential in infants with compromised microbiota development, and that these infants could benefit from corrective supplementation.
30214024	7	65	theme	microbiota	948:957	arg1	patterns					959:966	the caesarean-associated microbiota patterns	923:966	the caesarean-associated microbiota patterns	923:966	In the caesarean-born, however, many of the caesarean-associated microbiota patterns were more pronounced among the infants of non-secretor mothers compared to those of secretor mothers.
30214024	3	66	theme	non-secretor	480:491	arg1	mothers					493:499	non-secretor mothers	480:499	non-secretor mothers	480:499	Secretor mothers produce milk containing α1-2 fucosylated human milk oligosaccharides, which are absent in the milk of non-secretor mothers.
30214024	4	67	theme	milk	583:586	arg1	HMOs					606:609	HMOs	606:609	HMOs	606:609	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	4	67	theme	milk	583:586	arg1	oligosaccharides					588:603	human milk oligosaccharides	577:603	human milk oligosaccharides (HMOs)	577:610	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	5	68	theme	non-secretor	716:727	arg1	mothers					738:744	secretor (N = 76) and non-secretor (N = 15) mothers	694:744	mothers	738:744	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	9	69	theme	mothers	1284:1290	arg1	infants					1264:1270	the section-born infants	1247:1270	the section-born infants of secretor mothers	1247:1290	Furthermore, Akkermansia was increased in the section-born infants of secretor mothers, supporting the suggestion that this organism may degrade HMOs.
30214024	5	70	from	differences	637:647	arg1	composition					674:684	infant gut microbiota composition	652:684	infant gut microbiota composition	652:684	Here we investigate the differences in infant gut microbiota composition between secretor (N = 76) and non-secretor (N = 15) mothers, taking into account birth mode.
30214024	2	71	theme	secretor	335:342	arg1	genotype					351:358	maternal secretor (FUT2) genotype	326:358	maternal secretor (FUT2) genotype	326:358	The oligosaccharide composition of breast milk varies between mothers, and is dependent on maternal secretor (FUT2) genotype.
30214024	4	72	theme	human	577:581	arg1	HMOs					606:609	HMOs	606:609	HMOs	606:609	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30214024	4	72	theme	human	577:581	arg1	oligosaccharides					588:603	human milk oligosaccharides	577:603	human milk oligosaccharides (HMOs)	577:610	Several strains of bacteria in the infant gut have the capacity to utilise human milk oligosaccharides (HMOs).
30529207	1	0	theme	oxidative	381:389	arg1	polymerization					391:404	oxidative polymerization	381:404	oxidative polymerization of dopamine (DA)	381:421	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	1	1	theme	acrylic	183:189	arg1	superabsorbent					211:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	1	2	theme	catecholamine	293:305	arg1	groups					318:323	then the catecholamine functional groups	284:323	then the catecholamine functional groups	284:323	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	1	3	theme	acid‑co‑acrylamide	191:208	arg1	superabsorbent					211:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	1	4	theme	functional	307:316	arg1	groups					318:323	then the catecholamine functional groups	284:323	then the catecholamine functional groups	284:323	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	4	5	with	adsorbent	734:742	arg1	ratio					767:771	5914.66% swelling ratio	749:771	5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	749:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	1	6	theme	dopamine	409:416	arg1	polymerization					391:404	oxidative polymerization	381:404	oxidative polymerization of dopamine (DA)	381:421	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	5	7	theme	adsorption	933:942	arg1	capacity					944:951	The maximum adsorption capacity	921:951	The maximum adsorption capacity	921:951	The maximum adsorption capacity was obtained 2276 mg g-1 at pH 9 and within 100 min.
30529207	2	8	theme	characterization	546:561	arg1	techniques					563:572	different characterization techniques	536:572	different characterization techniques	536:572	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	4	9	theme	methylene	869:877	arg1	adsorption					855:864	the adsorption	851:864	the adsorption of methylene blue (MB)	851:887	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	10	theme	surface	808:814	arg1	area					816:819	a specific surface area	797:819	a specific surface area of 2.8031 m2 g-1	797:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	5	11	theme	maximum	925:931	arg1	capacity					944:951	The maximum adsorption capacity	921:951	The maximum adsorption capacity	921:951	The maximum adsorption capacity was obtained 2276 mg g-1 at pH 9 and within 100 min.
30529207	6	12	theme	environmental	1104:1116	arg1	points					1130:1135	different environmental remediation points	1094:1135	different environmental remediation points of view	1094:1143	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	2	13	theme	different	536:544	arg1	techniques					563:572	different characterization techniques	536:572	different characterization techniques	536:572	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	4	14	theme	specific	799:806	arg1	area					816:819	a specific surface area	797:819	a specific surface area of 2.8031 m2 g-1	797:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	15	used	used	842:845	arg2	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	15	used	used	842:845	arg2	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	6	16	theme	different	1094:1102	arg1	points					1130:1135	different environmental remediation points	1094:1135	different environmental remediation points of view	1094:1143	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	0	17	theme	catecholamine/starch	12:31	arg1	composites					33:42	catecholamine/starch composites	12:42	catecholamine/starch composites	12:42	Bioinspired catecholamine/starch composites as superadsorbent for the environmental remediation.
30529207	6	18	theme	remediation	1118:1128	arg1	points					1130:1135	different environmental remediation points	1094:1135	different environmental remediation points of view	1094:1143	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	4	19	theme	blue	879:882	arg1	methylene					869:877	methylene blue	869:882	methylene blue (MB)	869:887	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	19	theme	blue	879:882	arg1	MB					885:886	MB	885:886	MB	885:886	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	1	20	theme	encouraging	113:123	arg1	properties					125:134	the encouraging properties	109:134	the encouraging properties of starch-based composite materials	109:170	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	4	21	theme	bimodal	715:721	arg1	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	21	theme	bimodal	715:721	arg1	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	6	22	with	adsorbent	1010:1018	arg1	capacity					1061:1068	unprecedented super high adsorption capacity	1025:1068	unprecedented super high adsorption capacity	1025:1068	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	4	23	theme	swelling	758:765	arg1	ratio					767:771	5914.66% swelling ratio	749:771	5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	749:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	3	24	theme	coating	598:604	arg1	thickness					606:614	The polydopamine (PDA) coating thickness	575:614	The polydopamine (PDA) coating thickness	575:614	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	1	25	theme	solution	248:255	arg1	polymerization					257:270	solution polymerization	248:270	solution polymerization method	248:277	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	2	26	theme	conditions	503:512	arg1	terms					455:459	terms	455:459	terms of the monomers' mass ratio and synthesis conditions	455:512	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	1	27	theme	pore	347:350	arg1	surface					352:358	pore surface	347:358	pore surface of the absorbent via oxidative polymerization of dopamine (DA)	347:421	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	3	28	theme	transmission	636:647	arg1	TEM					670:672	TEM	670:672	TEM	670:672	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	3	28	theme	transmission	636:647	arg1	microscopy					658:667	transmission electron microscopy	636:667	transmission electron microscopy (TEM) image	636:679	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	1	29	theme	polymerization	257:270	arg1	method					272:277	solution polymerization method	248:277	solution polymerization method	248:277	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	2	30	theme	synthesis	493:501	arg1	conditions					503:512	synthesis conditions	493:512	synthesis conditions	493:512	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	4	31	with	micropores	781:790	arg1	area					816:819	a specific surface area	797:819	a specific surface area of 2.8031 m2 g-1	797:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	3	32	theme	electron	649:656	arg1	TEM					670:672	TEM	670:672	TEM	670:672	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	3	32	theme	electron	649:656	arg1	microscopy					658:667	transmission electron microscopy	636:667	transmission electron microscopy (TEM) image	636:679	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	6	33	theme	adsorption	1050:1059	arg1	capacity					1061:1068	unprecedented super high adsorption capacity	1025:1068	unprecedented super high adsorption capacity	1025:1068	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	0	34	theme	environmental	70:82	arg1	remediation					84:94	the environmental remediation	66:94	the environmental remediation	66:94	Bioinspired catecholamine/starch composites as superadsorbent for the environmental remediation.
30529207	4	35	theme	water	900:904	arg1	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	35	theme	water	900:904	arg1	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	6	36	theme	view	1140:1143	arg1	points					1130:1135	different environmental remediation points	1094:1135	different environmental remediation points of view	1094:1143	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	1	37	theme	starch-based	139:150	arg1	materials					162:170	starch-based composite materials	139:170	starch-based composite materials	139:170	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	2	38	theme	ratio	483:487	arg1	terms					455:459	terms	455:459	terms of the monomers' mass ratio and synthesis conditions	455:512	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	4	39	theme	model	894:898	arg1	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	39	theme	model	894:898	arg1	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	1	40	theme	composite	152:160	arg1	materials					162:170	starch-based composite materials	139:170	starch-based composite materials	139:170	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	1	41	theme	absorbent	367:375	arg1	surface					352:358	pore surface	347:358	pore surface of the absorbent via oxidative polymerization of dopamine (DA)	347:421	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	6	42	theme	super	1039:1043	arg1	capacity					1061:1068	unprecedented super high adsorption capacity	1025:1068	unprecedented super high adsorption capacity	1025:1068	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	1	43	theme	materials	162:170	arg1	properties					125:134	the encouraging properties	109:134	the encouraging properties of starch-based composite materials	109:170	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	6	44	theme	high	1045:1048	arg1	capacity					1061:1068	unprecedented super high adsorption capacity	1025:1068	unprecedented super high adsorption capacity	1025:1068	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	4	45	theme	pollutant	906:914	arg1	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	45	theme	pollutant	906:914	arg1	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	6	46	from	points	1130:1135	arg1	adsorbent					1010:1018	adsorbent	1010:1018	adsorbent	1010:1018	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	6	46	from	points	1130:1135	arg1	encouraging					1077:1087	encouraging	1077:1087	encouraging	1077:1087	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	4	47	theme	5914.66	749:755	arg1	%					756:756	%	756:756	%	756:756	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	48	theme	%	756:756	arg1	swelling					758:765	5914.66% swelling	749:765	5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	749:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	3	49	theme	microscopy	658:667	arg1	image					675:679	transmission electron microscopy (TEM) image	636:679	transmission electron microscopy (TEM) image	636:679	The polydopamine (PDA) coating thickness was estimated using transmission electron microscopy (TEM) image and it was found to be 83 nm.
30529207	1	50	theme	starch‑g‑	173:181	arg1	superabsorbent					211:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent	173:224	Focusing on the encouraging properties of starch-based composite materials, starch‑g‑(acrylic acid‑co‑acrylamide) superabsorbent was synthesized using solution polymerization method, and then the catecholamine functional groups were introduced on to pore surface of the absorbent via oxidative polymerization of dopamine (DA).
30529207	6	51	theme	unprecedented	1025:1037	arg1	capacity					1061:1068	unprecedented super high adsorption capacity	1025:1068	unprecedented super high adsorption capacity	1025:1068	The adsorbent with unprecedented super high adsorption capacity can be encouraging from different environmental remediation points of view.
30529207	4	52	theme	2.8031 m2 g-1	824:836	arg1	area					816:819	a specific surface area	797:819	a specific surface area of 2.8031 m2 g-1	797:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	2	53	theme	mass	478:481	arg1	ratio					483:487	the monomers' mass ratio	464:487	the monomers' mass ratio	464:487	The adsorbent was optimized in terms of the monomers' mass ratio and synthesis conditions, and characterized by different characterization techniques.
30529207	4	54	theme	adsorbent	734:742	arg1	dye					916:918	a model water pollutant dye	892:918	a model water pollutant dye	892:918	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
30529207	4	54	theme	adsorbent	734:742	arg1	mesoporous					723:732	The bimodal mesoporous	711:732	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1	711:836	The bimodal mesoporous adsorbent with 5914.66% swelling ratio bearing micropores with a specific surface area of 2.8031 m2 g-1 was used for the adsorption of methylene blue (MB) as a model water pollutant dye.
29915316	2	0	theme	direct	472:477	arg1	incorporation					487:499	direct further incorporation	472:499	direct further incorporation during the following impregnation procedure	472:543	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	4	1	theme	potential	861:869	arg1	applications					871:882	potential applications	861:882	potential applications	861:882	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	1	2	theme	hollow	146:151	arg1	nanospheres					175:185	hollow noble metal composite nanospheres	146:185	hollow noble metal composite nanospheres within the ordered array nanostructures	146:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	1	3	theme	noble	153:157	arg1	nanospheres					175:185	hollow noble metal composite nanospheres	146:185	hollow noble metal composite nanospheres within the ordered array nanostructures	146:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	4	4	contain	have	856:859	arg1	which					844:848	which	844:848	which	844:848	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	4	contain	have	856:859	arg2	applications					871:882	potential applications	861:882	potential applications	861:882	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	2	5	theme	impregnation	522:533	arg1	procedure					535:543	the following impregnation procedure	508:543	the following impregnation procedure	508:543	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	3	6	theme	Butterfly	546:554	arg1	wings					556:560	Butterfly wings	546:560	Butterfly wings	546:560	Butterfly wings could function as bio-substrate to provide an ordered array and regulate the synthesis process by providing active reaction sites (e.g. -CONH- and -OH).
29915316	1	7	theme	convenient	266:275	arg1	procedure					287:295	some convenient technique procedure	261:295	some convenient technique procedure	261:295	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	2	8	theme	following	512:520	arg1	procedure					535:543	the following impregnation procedure	508:543	the following impregnation procedure	508:543	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	2	9	theme	Butterfly	298:306	arg1	wings					308:312	Butterfly wings	298:312	Butterfly wings	298:312	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	1	10	theme	technique	277:285	arg1	procedure					287:295	some convenient technique procedure	261:295	some convenient technique procedure	261:295	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	4	11	dep	layer	779:783	arg1	nanostructures					814:827	the ordered array nanostructures	796:827	the ordered array nanostructures	796:827	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	1	12	theme	metal	159:163	arg1	nanospheres					175:185	hollow noble metal composite nanospheres	146:185	hollow noble metal composite nanospheres within the ordered array nanostructures	146:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	1	13	theme	composite	165:173	arg1	nanospheres					175:185	hollow noble metal composite nanospheres	146:185	hollow noble metal composite nanospheres within the ordered array nanostructures	146:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	2	14	from	sites	372:376	arg1	component					392:400	the chitin component	381:400	the chitin component	381:400	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	1	15	theme	nanospheres	175:185	arg1	fabrication					131:141	the fabrication	127:141	the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures	127:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	3	16	dep	-CONH-	698:703	arg1	e.g.					693:696	e.g.	693:696	e.g.	693:696	Butterfly wings could function as bio-substrate to provide an ordered array and regulate the synthesis process by providing active reaction sites (e.g. -CONH- and -OH).
29915316	1	17	theme	biological	79:88	arg1	strategy					99:106	A biological template strategy	77:106	A biological template strategy	77:106	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	0	18	theme	Ag-Au	19:23	arg1	Nanospheres					25:35	Hollow Ag-Au Nanospheres	12:35	Hollow Ag-Au Nanospheres	12:35	Ordering of Hollow Ag-Au Nanospheres with Butterfly Wings as a Bio-template.
29915316	4	19	theme	Ag-Au	728:732	arg1	nanospheres					734:744	hollow Ag-Au nanospheres	721:744	hollow Ag-Au nanospheres	721:744	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	1	20	theme	template	90:97	arg1	strategy					99:106	A biological template strategy	77:106	A biological template strategy	77:106	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	0	21	theme	Hollow	12:17	arg1	Nanospheres					25:35	Hollow Ag-Au Nanospheres	12:35	Hollow Ag-Au Nanospheres	12:35	Ordering of Hollow Ag-Au Nanospheres with Butterfly Wings as a Bio-template.
29915316	1	22	theme	ordered	198:204	arg1	nanostructures					212:225	the ordered array nanostructures	194:225	the ordered array nanostructures	194:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	4	23	theme	ordered	800:806	arg1	nanostructures					814:827	the ordered array nanostructures	796:827	the ordered array nanostructures	796:827	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	1	24	theme	array	206:210	arg1	nanostructures					212:225	the ordered array nanostructures	194:225	the ordered array nanostructures	194:225	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	0	25	theme	Nanospheres	25:35	arg1	Ordering					0:7	Ordering	0:7	Ordering of Hollow Ag-Au Nanospheres with Butterfly	0:50	Ordering of Hollow Ag-Au Nanospheres with Butterfly Wings as a Bio-template.
29915316	2	26	theme	seeds	462:466	arg1	"					467:467	"seeds"	461:467	"seeds" to direct further incorporation during the following impregnation procedure	461:543	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	3	27	theme	synthesis	639:647	arg1	process					649:655	the synthesis process	635:655	the synthesis process	635:655	Butterfly wings could function as bio-substrate to provide an ordered array and regulate the synthesis process by providing active reaction sites (e.g. -CONH- and -OH).
29915316	3	28	theme	ordered	608:614	arg1	array					616:620	an ordered array	605:620	an ordered array	605:620	Butterfly wings could function as bio-substrate to provide an ordered array and regulate the synthesis process by providing active reaction sites (e.g. -CONH- and -OH).
29915316	2	29	theme	Ag	412:413	arg1	nanoparticles					415:427	Ag nanoparticles	412:427	Ag nanoparticles	412:427	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	4	30	theme	enhanced	895:902	arg1	SERS					922:925	SERS	922:925	SERS	922:925	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	30	theme	enhanced	895:902	arg1	scattering					910:919	surface enhanced Raman scattering	887:919	surface enhanced Raman scattering (SERS)	887:926	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	2	31	theme	reactive	363:370	arg1	sites					372:376	the reactive sites	359:376	the reactive sites	359:376	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	4	32	theme	surface	887:893	arg1	SERS					922:925	SERS	922:925	SERS	922:925	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	32	theme	surface	887:893	arg1	scattering					910:919	surface enhanced Raman scattering	887:919	surface enhanced Raman scattering (SERS)	887:926	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	33	theme	array	808:812	arg1	nanostructures					814:827	the ordered array nanostructures	796:827	the ordered array nanostructures	796:827	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	0	34	with	Ordering	0:7	arg1	Butterfly					42:50	Butterfly	42:50	Butterfly	42:50	Ordering of Hollow Ag-Au Nanospheres with Butterfly Wings as a Bio-template.
29915316	4	35	theme	hollow	721:726	arg1	nanospheres					734:744	hollow Ag-Au nanospheres	721:744	hollow Ag-Au nanospheres	721:744	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	36	theme	Raman	904:908	arg1	SERS					922:925	SERS	922:925	SERS	922:925	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	4	36	theme	Raman	904:908	arg1	scattering					910:919	surface enhanced Raman scattering	887:919	surface enhanced Raman scattering (SERS)	887:926	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	3	37	theme	active	670:675	arg1	reaction sites					677:690	active reaction sites	670:690	active reaction sites (e.g. -CONH- and -OH)	670:712	Butterfly wings could function as bio-substrate to provide an ordered array and regulate the synthesis process by providing active reaction sites (e.g. -CONH- and -OH).
29915316	4	38	theme	surface	771:777	arg1	layer					779:783	the wings' surface layer	760:783	the wings' surface layer	760:783	Thus, hollow Ag-Au nanospheres are loaded on the wings' surface layer and inside the ordered array nanostructures homogeneously, which would have potential applications in surface enhanced Raman scattering (SERS).
29915316	2	39	theme	chitin	385:390	arg1	component					392:400	the chitin component	381:400	the chitin component	381:400	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
29915316	1	40	theme	butterfly	242:250	arg1	wings					252:256	butterfly wings	242:256	butterfly wings	242:256	A biological template strategy is implemented for the fabrication of hollow noble metal composite nanospheres within the ordered array nanostructures by introducing butterfly wings to some convenient technique procedure.
29915316	2	41	theme	further	479:485	arg1	incorporation					487:499	direct further incorporation	472:499	direct further incorporation during the following impregnation procedure	472:543	Butterfly wings are activated by ethylenediamine to increase the reactive sites on the chitin component, on which Ag nanoparticles are in situ formed and serve as "seeds" to direct further incorporation during the following impregnation procedure.
31726161	0	0	theme	composite	98:106	arg1	film					108:111	essential oil composite film	84:111	essential oil composite film	84:111	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	2	1	theme	SC/GG	458:462	arg1	film					464:467	the SC/GG film	454:467	the SC/GG film either individually or in combination	454:505	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	1	2	theme	cumin	256:260	arg1	oil					272:274	cumin essential oil	256:274	cumin essential oil	256:274	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	2	3	dep	film	464:467	arg1	combination					495:505	combination	495:505	combination	495:505	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	3	dep	film	464:467	arg1	either					469:474	either	469:474	either	469:474	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	4	4	dep	Gram-negative	890:902	arg1	enteritidis					929:939	S. enteritidis	926:939	S. enteritidis	926:939	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	4	dep	Gram-negative	890:902	arg1	O157					913:916	O157	913:916	E. coli O157: H7	905:920	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	1	5	theme	essential	262:270	arg1	oil					272:274	cumin essential oil	256:274	cumin essential oil	256:274	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	2	6	theme	composite	355:363	arg1	film					365:368	SC/GG composite film	349:368	SC/GG composite film	349:368	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	3	7	theme	composite	712:720	arg1	films					722:726	the composite films	708:726	the composite films	708:726	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	TS					663:664	TS	663:664	TS	663:664	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	flexibility					688:698	flexibility	688:698	flexibility (EB)	688:703	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	stiffness					668:676	stiffness	668:676	stiffness (YM)	668:681	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	strength					653:660	the strength	649:660	the strength (TS)	649:665	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	YM					679:680	YM	679:680	YM	679:680	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	8	theme	films	722:726	arg1	EB					701:702	EB	701:702	EB	701:702	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	2	9	theme	film	365:368	arg1	preparation					334:344	the preparation	330:344	the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC)	330:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	1	10	theme	guar	207:210	arg1	matrix					236:241	the polymer matrix	224:241	the polymer matrix	224:241	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	10	theme	guar	207:210	arg1	GG					217:218	GG	217:218	GG	217:218	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	10	theme	guar	207:210	arg1	gum					212:214	guar gum	207:214	guar gum (GG)	207:219	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	10	theme	guar	207:210	arg1	fillers					296:302	functional fillers	285:302	functional fillers	285:302	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	10	theme	guar	207:210	arg1	caseinate					188:196	sodium caseinate	181:196	sodium caseinate (SC)	181:201	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	4	11	dep	Gram-positive	839:851	arg1	monocytogenes					857:869	L. monocytogenes	854:869	L. monocytogenes	854:869	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	11	dep	Gram-positive	839:851	arg1	aureus					878:883	S. aureus	875:883	S. aureus	875:883	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	11	dep	Gram-positive	839:851	arg1	bacteria					942:949	bacteria	942:949	bacteria	942:949	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	12	theme	SC/GG	739:743	arg1	films					745:749	the SC/GG films	735:749	the SC/GG films incorporated with TiO2 and CEO	735:780	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	1	13	theme	Eco-friendly	114:125	arg1	films					155:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	2	14	theme	SC/GG	349:353	arg1	film					365:368	SC/GG composite film	349:368	SC/GG composite film	349:368	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	15	theme	CEO	401:403	arg1	film					365:368	SC/GG composite film	349:368	SC/GG composite film	349:368	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	15	theme	CEO	401:403	arg1	amount					382:387	various amount	374:387	various amount of TiO2 and CEO (1 and 2 wt% based on SC)	374:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	15	theme	CEO	401:403	arg1	CEO					401:403	CEO	401:403	CEO	401:403	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	15	theme	CEO	401:403	arg1	TiO2					392:395	TiO2	392:395	TiO2	392:395	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	1	16	theme	functional	127:136	arg1	films					155:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	2	17	theme	TiO2	392:395	arg1	film					365:368	SC/GG composite film	349:368	SC/GG composite film	349:368	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	17	theme	TiO2	392:395	arg1	amount					382:387	various amount	374:387	various amount of TiO2 and CEO (1 and 2 wt% based on SC)	374:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	17	theme	TiO2	392:395	arg1	CEO					401:403	CEO	401:403	CEO	401:403	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	17	theme	TiO2	392:395	arg1	TiO2					392:395	TiO2	392:395	TiO2	392:395	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	5	18	dep	only	992:995	arg1	not					988:990	not	988:990	not	988:990	All the film properties were varied not only on the concentration of TiO2 and CEO, but also increased synergistically when they were added together.
31726161	1	19	theme	bionanocomposite	138:153	arg1	films					155:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films	114:159	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	0	20	theme	sodium	47:52	arg1	caseinate/guar					54:67	functional sodium caseinate/guar	36:67	functional sodium caseinate/guar	36:67	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	3	21	theme	sensitivity	587:597	arg1	stiffness					668:676	stiffness	668:676	stiffness (YM)	668:681	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	21	theme	sensitivity	587:597	arg1	strength					653:660	the strength	649:660	the strength (TS)	649:665	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	21	theme	sensitivity	587:597	arg1	flexibility					688:698	flexibility	688:698	flexibility (EB)	688:703	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	21	theme	sensitivity	587:597	arg1	properties					599:608	sensitivity properties	587:608	sensitivity properties	587:608	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	22	theme	TiO2	524:527	arg1	addition					512:519	The addition	508:519	The addition of TiO2 and CEO	508:535	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	2	23	theme	amount	382:387	arg1	preparation					334:344	the preparation	330:344	the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC)	330:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	0	24	theme	functional	36:45	arg1	caseinate/guar					54:67	functional sodium caseinate/guar	36:67	functional sodium caseinate/guar	36:67	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	4	25	theme	E.	905:906	arg1	O157					913:916	O157	913:916	E. coli O157: H7	905:920	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	26	dep	O157	913:916	arg1	coli					908:911	E. coli O157: H7	905:920	E. coli O157: H7	905:920	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	4	26	dep	O157	913:916	arg1	H7					919:920	H7	919:920	E. coli O157: H7	905:920	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	3	27	theme	water	564:568	arg1	permeability					570:581	the water permeability	560:581	the water permeability	560:581	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	2	28	theme	various	374:380	arg1	amount					382:387	various amount	374:387	various amount of TiO2 and CEO (1 and 2 wt% based on SC)	374:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	28	theme	various	374:380	arg1	CEO					401:403	CEO	401:403	CEO	401:403	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	28	theme	various	374:380	arg1	TiO2					392:395	TiO2	392:395	TiO2	392:395	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	1	29	theme	functional	285:294	arg1	caseinate					188:196	sodium caseinate	181:196	sodium caseinate (SC)	181:201	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	29	theme	functional	285:294	arg1	gum					212:214	guar gum	207:214	guar gum (GG)	207:219	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	29	theme	functional	285:294	arg1	fillers					296:302	functional fillers	285:302	functional fillers	285:302	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	5	30	theme	film	960:963	arg1	properties					965:974	All the film properties	952:974	All the film properties	952:974	All the film properties were varied not only on the concentration of TiO2 and CEO, but also increased synergistically when they were added together.
31726161	2	31	dep	TiO2	392:395	arg1	%					416:416	1 and 2 wt%	406:416	1 and 2 wt% based on SC	406:428	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	2	32	dep	either	469:474	arg1	individually					476:487	individually	476:487	individually	476:487	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	0	33	theme	caseinate/guar	54:67	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	0	33	theme	caseinate/guar	54:67	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	3	34	theme	mechanical	614:623	arg1	stiffness					668:676	stiffness	668:676	stiffness (YM)	668:681	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	34	theme	mechanical	614:623	arg1	strength					653:660	the strength	649:660	the strength (TS)	649:665	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	34	theme	mechanical	614:623	arg1	flexibility					688:698	flexibility	688:698	flexibility (EB)	688:703	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	3	34	theme	mechanical	614:623	arg1	characteristics					625:639	mechanical characteristics	614:639	mechanical characteristics	614:639	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	5	35	theme	CEO	1030:1032	arg1	concentration					1004:1016	the concentration	1000:1016	the concentration of TiO2 and CEO	1000:1032	All the film properties were varied not only on the concentration of TiO2 and CEO, but also increased synergistically when they were added together.
31726161	4	36	theme	antibacterial	803:815	arg1	activity					817:824	remarkable antibacterial activity	792:824	remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria	792:949	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	2	37	theme	%	312:312	arg1	GG					314:315	0.2 vol% GG	305:315	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC)	305:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	4	38	theme	remarkable	792:801	arg1	activity					817:824	remarkable antibacterial activity	792:824	remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria	792:949	Also, the SC/GG films incorporated with TiO2 and CEO exhibited remarkable antibacterial activity against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli O157: H7 and S. enteritidis) bacteria.
31726161	2	39	theme	0.2 vol	305:311	arg1	GG					314:315	0.2 vol% GG	305:315	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC)	305:429	0.2 vol% GG selected for the preparation of SC/GG composite film and various amount of TiO2 and CEO (1 and 2 wt% based on SC) were incorporated into the SC/GG film either individually or in combination.
31726161	1	40	theme	polymer	228:234	arg1	matrix					236:241	the polymer matrix	224:241	the polymer matrix	224:241	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	40	theme	polymer	228:234	arg1	caseinate					188:196	sodium caseinate	181:196	sodium caseinate (SC)	181:201	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	40	theme	polymer	228:234	arg1	gum					212:214	guar gum	207:214	guar gum (GG)	207:219	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	0	41	theme	oil	94:96	arg1	film					108:111	essential oil composite film	84:111	essential oil composite film	84:111	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	3	42	theme	CEO	533:535	arg1	addition					512:519	The addition	508:519	The addition of TiO2 and CEO	508:535	The addition of TiO2 and CEO significantly improved the water permeability and sensitivity properties and mechanical characteristics such as the strength (TS), stiffness (YM), and flexibility (EB) of the composite films.
31726161	1	43	theme	sodium	181:186	arg1	matrix					236:241	the polymer matrix	224:241	the polymer matrix	224:241	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	43	theme	sodium	181:186	arg1	caseinate					188:196	sodium caseinate	181:196	sodium caseinate (SC)	181:201	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	43	theme	sodium	181:186	arg1	gum					212:214	guar gum	207:214	guar gum (GG)	207:219	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	43	theme	sodium	181:186	arg1	fillers					296:302	functional fillers	285:302	functional fillers	285:302	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	1	43	theme	sodium	181:186	arg1	SC					199:200	SC	199:200	SC	199:200	Eco-friendly functional bionanocomposite films were prepared using sodium caseinate (SC) and guar gum (GG) as the polymer matrix and TiO2 and cumin essential oil (CEO) as functional fillers.
31726161	0	44	theme	essential	84:92	arg1	film					108:111	essential oil composite film	84:111	essential oil composite film	84:111	Preparation and characterization of functional sodium caseinate/guar gum/TiO2/cumin essential oil composite film.
31726161	5	45	theme	TiO2	1021:1024	arg1	concentration					1004:1016	the concentration	1000:1016	the concentration of TiO2 and CEO	1000:1032	All the film properties were varied not only on the concentration of TiO2 and CEO, but also increased synergistically when they were added together.
30867744	10	0	from	tumors	1700:1705	arg1	assessment					1638:1647	The assessment	1634:1647	The assessment of its expression in cancer cell lines and patient tumors	1634:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	0	1	theme	glycans	40:46	arg1	Expression					0:9	Expression	0:9	Expression of bladder cancer-associated glycans in murine tumor cell lines.	0:74	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	12	2	theme	available	2158:2166	arg1	lines					2152:2156	the two murine cell lines	2132:2156	the two murine cell lines available for the development of preclinical studies in bladder cancer	2132:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	8	3	theme	NGcGM3	1495:1500	arg1	expression					1481:1490	the expression	1477:1490	the expression of NGcGM3 in human or murine bladder cancer	1477:1534	There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer.
30867744	12	4	from	studies	2203:2209	arg1	cancer					2222:2227	bladder cancer	2214:2227	bladder cancer	2214:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	4	5	from	prognosis	618:626	arg1	cancer					645:650	human bladder cancer	631:650	human bladder cancer	631:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	5	6	theme	MB49-I	1073:1078	arg1	cells					1080:1084	MB49-I cells	1073:1084	MB49-I cells	1073:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	8	7	theme	bladder	1521:1527	arg1	cancer					1529:1534	human or murine bladder cancer	1505:1534	human or murine bladder cancer	1505:1534	There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer.
30867744	1	8	theme	preclinical	162:172	arg1	models					174:179	preclinical models	162:179	preclinical models that could resemble human diseases	162:214	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	6	9	located	detected	1263:1270	arg1	line					1287:1290	either cell line	1275:1290	line	1287:1290	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	6	9	located	detected	1263:1270	arg2	expression					1237:1246	The expression	1233:1246	The expression of LeX	1233:1253	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	6	10	theme	lines	1380:1384	arg1	monolayers					1323:1332	monolayers	1323:1332	monolayers	1323:1332	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	6	10	theme	lines	1380:1384	arg1	spheroids					1335:1343	spheroids	1335:1343	spheroids	1335:1343	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	6	10	theme	lines	1380:1384	arg1	tumors					1360:1365	orthotopic tumors	1349:1365	orthotopic tumors	1349:1365	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	10	11	from	relevance	1747:1755	arg1	biology					1786:1792	tumor biology	1780:1792	tumor biology	1780:1792	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	2	12	theme	glycans	292:298	arg1	expression					278:287	excessive or novel expression	259:287	excessive or novel expression of glycans	259:298	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	2	12	theme	glycans	292:298	arg1	loss					253:256	the loss	249:256	the loss	249:256	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	2	12	theme	glycans	292:298	arg1	appearance					308:317	the appearance	304:317	the appearance of truncated structures	304:341	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	5	13	from	biology	907:913	arg1	relevance					877:885	the relevance	873:885	the relevance of glycans in tumor biology	873:913	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	12	14	theme	cell	2147:2150	arg1	lines					2152:2156	the two murine cell lines	2132:2156	the two murine cell lines available for the development of preclinical studies in bladder cancer	2132:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	5	15	theme	therapies	963:971	arg1	they					936:939	they	936:939	they	936:939	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	15	theme	therapies	963:971	arg1	targets					952:958	targets	952:958	targets of therapies and biomarkers	952:986	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	16	theme	STn	1046:1048	arg1	expression					1021:1030	the expression	1017:1030	the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells	1017:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	6	17	theme	cell	1375:1378	arg1	lines					1380:1384	both cell lines	1370:1384	both cell lines	1370:1384	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	10	18	theme	cancer	1670:1675	arg1	lines					1682:1686	cancer cell lines	1670:1686	cancer cell lines	1670:1686	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	1	19	theme	great	124:128	arg1	importance					130:139	great importance	124:139	great importance	124:139	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	4	20	theme	human	631:635	arg1	cancer					645:650	human bladder cancer	631:650	human bladder cancer	631:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	5	21	theme	three-dimensional	1146:1162	arg1	spheroids					1178:1186	three-dimensional multicellular spheroids	1146:1186	three-dimensional multicellular spheroids	1146:1186	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	0	22	theme	cell	64:67	arg1	lines					69:73	murine tumor cell lines	51:73	murine tumor cell lines	51:73	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	10	23	theme	expression	1656:1665	arg1	assessment					1638:1647	The assessment	1634:1647	The assessment of its expression in cancer cell lines and patient tumors	1634:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	6	24	theme	LeX	1251:1253	arg1	expression					1237:1246	The expression	1233:1246	The expression of LeX	1233:1253	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	4	25	theme	cancer	845:850	arg1	type					837:840	this type	832:840	this type of cancer	832:850	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	0	26	from	Expression	0:9	arg1	lines					69:73	murine tumor cell lines	51:73	murine tumor cell lines	51:73	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	5	27	theme	biomarkers	977:986	arg1	they					936:939	they	936:939	they	936:939	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	27	theme	biomarkers	977:986	arg1	targets					952:958	targets	952:958	targets of therapies and biomarkers	952:986	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	10	28	from	assessment	1638:1647	arg1	lines					1682:1686	cancer cell lines	1670:1686	cancer cell lines	1670:1686	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	10	28	from	assessment	1638:1647	arg1	tumors					1700:1705	patient tumors	1692:1705	patient tumors	1692:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	3	29	theme	only	378:381	arg1	MB49-I					353:358	MB49-I	353:358	MB49-I	353:358	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	29	theme	only	378:381	arg1	lines					399:403	the only two murine cell lines	374:403	the only two murine cell lines available for the development of preclinical bladder cancer models	374:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	29	theme	only	378:381	arg1	MB49					344:347	MB49	344:347	MB49	344:347	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	11	30	theme	cancer	1997:2002	arg1	models					2004:2009	murine cancer models	1990:2009	murine cancer models	1990:2009	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	12	31	theme	preclinical	2191:2201	arg1	studies					2203:2209	preclinical studies	2191:2209	preclinical studies in bladder cancer	2191:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	12	32	theme	present	2046:2052	arg1	study					2054:2058	the present study	2042:2058	the present study	2042:2058	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	12	32	theme	present	2046:2052	arg1	first					2067:2071	first	2067:2071	first	2067:2071	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	3	33	theme	cancer	458:463	arg1	models					465:470	preclinical bladder cancer models	438:470	preclinical bladder cancer models	438:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	0	34	theme	bladder	14:20	arg1	glycans					40:46	bladder cancer-associated glycans	14:46	bladder cancer-associated glycans	14:46	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	11	35	theme	present	1820:1826	arg1	study					1828:1832	the present study	1816:1832	the present study	1816:1832	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	1	36	theme	cell	107:110	arg1	lines					112:116	murine cell lines	100:116	murine cell lines	100:116	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	10	37	theme	patient	1692:1698	arg1	tumors					1700:1705	patient tumors	1692:1705	patient tumors	1692:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	5	38	theme	mouse	1192:1196	arg1	tumors					1225:1230	mouse heterotopic and orthotopic tumors	1192:1230	mouse heterotopic and orthotopic tumors	1192:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	3	39	theme	preclinical	438:448	arg1	models					465:470	preclinical bladder cancer models	438:470	preclinical bladder cancer models	438:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	5	40	from	expression	1021:1030	arg1	MB49					1064:1067	MB49	1064:1067	MB49	1064:1067	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	40	from	expression	1021:1030	arg1	cells					1080:1084	MB49-I cells	1073:1084	MB49-I cells	1073:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	6	41	theme	cell	1282:1285	arg1	line					1287:1290	either cell line	1275:1290	line	1287:1290	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	3	42	theme	murine	387:392	arg1	MB49-I					353:358	MB49-I	353:358	MB49-I	353:358	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	42	theme	murine	387:392	arg1	lines					399:403	the only two murine cell lines	374:403	the only two murine cell lines available for the development of preclinical bladder cancer models	374:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	42	theme	murine	387:392	arg1	MB49					344:347	MB49	344:347	MB49	344:347	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	0	43	theme	murine	51:56	arg1	lines					69:73	murine tumor cell lines	51:73	murine tumor cell lines	51:73	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	4	44	dep	associated	563:572	arg1	neo-antigen					695:705	a neo-antigen	693:705	a neo-antigen expressed in many types of tumors	693:739	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	44	dep	associated	563:572	arg1	GM3					677:679	N-glycolyl GM3	666:679	N-glycolyl GM3 (NGcGM3)	666:688	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	45	theme	lewis	507:511	arg1	glycans					477:483	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	45	theme	lewis	507:511	arg1	SLeX					516:519	SLeX	516:519	SLeX	516:519	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	45	theme	lewis	507:511	arg1	X					513:513	Sialyl lewis X	500:513	Sialyl lewis X (SLeX)	500:520	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	10	46	theme	great	1713:1717	arg1	importance					1719:1728	great importance	1713:1728	great importance	1713:1728	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	2	47	theme	truncated	322:330	arg1	structures					332:341	truncated structures	322:341	truncated structures	322:341	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	6	48	theme	orthotopic	1349:1358	arg1	tumors					1360:1365	orthotopic tumors	1349:1365	orthotopic tumors	1349:1365	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	5	49	theme	glycans	890:896	arg1	relevance					877:885	the relevance	873:885	the relevance of glycans in tumor biology	873:913	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	12	50	theme	studies	2203:2209	arg1	development					2176:2186	the development	2172:2186	the development of preclinical studies in bladder cancer	2172:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	4	51	theme	tumors	734:739	arg1	types					725:729	many types	720:729	many types of tumors	720:739	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	10	52	from	lines	1682:1686	arg1	assessment					1638:1647	The assessment	1634:1647	The assessment of its expression in cancer cell lines and patient tumors	1634:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	5	53	theme	tumor	901:905	arg1	biology					907:913	tumor biology	901:913	tumor biology	901:913	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	12	54	theme	bladder	2214:2220	arg1	cancer					2222:2227	bladder cancer	2214:2227	bladder cancer	2214:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	12	55	from	cancer	2222:2227	arg1	development					2176:2186	the development	2172:2186	the development of preclinical studies in bladder cancer	2172:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	5	56	from	relevance	877:885	arg1	biology					907:913	tumor biology	901:913	tumor biology	901:913	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	4	57	theme	Sialyl	526:531	arg1	glycans					477:483	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	57	theme	Sialyl	526:531	arg1	STn					537:539	STn	537:539	STn	537:539	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	57	theme	Sialyl	526:531	arg1	Tn					533:534	Sialyl Tn	526:534	Sialyl Tn (STn)	526:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	5	58	from	glycans	890:896	arg1	biology					907:913	tumor biology	901:913	tumor biology	901:913	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	10	59	theme	ganglioside	1765:1775	arg1	relevance					1747:1755	the relevance	1743:1755	the relevance of this ganglioside in tumor biology	1743:1792	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	2	60	theme	novel	272:276	arg1	expression					278:287	excessive or novel expression	259:287	excessive or novel expression of glycans	259:298	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	11	61	theme	murine	1990:1995	arg1	models					2004:2009	murine cancer models	1990:2009	murine cancer models	1990:2009	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	12	62	from	development	2176:2186	arg1	cancer					2222:2227	bladder cancer	2214:2227	bladder cancer	2214:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	4	63	from	dissemination	595:607	arg1	cancer					645:650	human bladder cancer	631:650	human bladder cancer	631:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	11	64	theme	characterization	1970:1985	arg1	importance					1952:1961	the importance	1948:1961	the importance of the characterization of murine cancer models	1948:2009	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	4	65	theme	poor	613:616	arg1	prognosis					618:626	poor prognosis	613:626	poor prognosis in human bladder cancer	613:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	11	66	theme	glycan	1852:1857	arg1	expression					1859:1868	glycan expression	1852:1868	glycan expression	1852:1868	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	4	67	from	aggressiveness	579:592	arg1	cancer					645:650	human bladder cancer	631:650	human bladder cancer	631:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	2	68	theme	excessive	259:267	arg1	expression					278:287	excessive or novel expression	259:287	excessive or novel expression of glycans	259:298	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	10	69	theme	tumor	1780:1784	arg1	biology					1786:1792	tumor biology	1780:1792	tumor biology	1780:1792	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	1	70	theme	human	201:205	arg1	diseases					207:214	human diseases	201:214	human diseases	201:214	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	9	71	theme	growth	1605:1610	arg1	conditions					1612:1621	all the growth conditions	1597:1621	all the growth conditions evaluated	1597:1631	In our hands, MB49 and MB49-I expressed this ganglioside in all the growth conditions evaluated.
30867744	2	72	theme	Aberrant	217:224	arg1	glycosylation					226:238	Aberrant glycosylation	217:238	Aberrant glycosylation	217:238	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	1	73	theme	lines	112:116	arg1	characterization					80:95	The characterization	76:95	The characterization of murine cell lines	76:116	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	12	74	theme	glycans	2120:2126	arg1	expression					2088:2097	the expression	2084:2097	the expression of cancer-associated glycans	2084:2126	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	5	75	theme	monolayer	1126:1134	arg1	cultures					1136:1143	monolayer cultures	1126:1143	monolayer cultures	1126:1143	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	8	76	from	expression	1481:1490	arg1	cancer					1529:1534	human or murine bladder cancer	1505:1534	human or murine bladder cancer	1505:1534	There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer.
30867744	10	77	theme	cell	1677:1680	arg1	lines					1682:1686	cancer cell lines	1670:1686	cancer cell lines	1670:1686	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	11	78	theme	growth	1916:1921	arg1	conditions					1923:1932	the growth conditions	1912:1932	the growth conditions	1912:1932	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	12	79	theme	murine	2140:2145	arg1	lines					2152:2156	the two murine cell lines	2132:2156	the two murine cell lines available for the development of preclinical studies in bladder cancer	2132:2227	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	4	80	theme	N-glycolyl	666:675	arg1	neo-antigen					695:705	a neo-antigen	693:705	a neo-antigen expressed in many types of tumors	693:739	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	80	theme	N-glycolyl	666:675	arg1	NGcGM3					682:687	NGcGM3	682:687	NGcGM3	682:687	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	80	theme	N-glycolyl	666:675	arg1	GM3					677:679	N-glycolyl GM3	666:679	N-glycolyl GM3 (NGcGM3)	666:688	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	12	81	theme	cancer-associated	2102:2118	arg1	glycans					2120:2126	cancer-associated glycans	2102:2126	cancer-associated glycans	2102:2126	To the best of our knowledge, the present study is the first to examine the expression of cancer-associated glycans, in the two murine cell lines available for the development of preclinical studies in bladder cancer.
30867744	5	82	theme	growth	1100:1105	arg1	cultures					1136:1143	monolayer cultures	1126:1143	monolayer cultures	1126:1143	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	82	theme	growth	1100:1105	arg1	tumors					1225:1230	mouse heterotopic and orthotopic tumors	1192:1230	mouse heterotopic and orthotopic tumors	1192:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	82	theme	growth	1100:1105	arg1	conditions					1107:1116	different growth conditions	1090:1116	different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors	1090:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	82	theme	growth	1100:1105	arg1	spheroids					1178:1186	three-dimensional multicellular spheroids	1146:1186	three-dimensional multicellular spheroids	1146:1186	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	7	83	theme	MB49	1414:1417	arg1	monolayers					1419:1428	MB49 monolayers	1414:1428	MB49 monolayers	1414:1428	STn was only identified in MB49 monolayers and spheroids.
30867744	4	84	theme	bladder	637:643	arg1	cancer					645:650	human bladder cancer	631:650	human bladder cancer	631:650	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	5	85	theme	heterotopic	1198:1208	arg1	tumors					1225:1230	mouse heterotopic and orthotopic tumors	1192:1230	mouse heterotopic and orthotopic tumors	1192:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	4	86	theme	Lewis	485:489	arg1	glycans					477:483	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	86	theme	Lewis	485:489	arg1	LeX					494:496	LeX	494:496	LeX	494:496	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	86	theme	Lewis	485:489	arg1	X					491:491	Lewis X	485:491	Lewis X (LeX)	485:497	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	5	87	theme	orthotopic	1214:1223	arg1	tumors					1225:1230	mouse heterotopic and orthotopic tumors	1192:1230	mouse heterotopic and orthotopic tumors	1192:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	88	theme	present	993:999	arg1	study					1001:1005	the present study	989:1005	the present study	989:1005	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	89	theme	multicellular	1164:1176	arg1	spheroids					1178:1186	three-dimensional multicellular spheroids	1146:1186	three-dimensional multicellular spheroids	1146:1186	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	0	90	theme	cancer-associated	22:38	arg1	glycans					40:46	bladder cancer-associated glycans	14:46	bladder cancer-associated glycans	14:46	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	4	91	dep	glycans	477:483	arg1	STn					537:539	STn	537:539	STn	537:539	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	LeX					494:496	LeX	494:496	LeX	494:496	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	SLeX					516:519	SLeX	516:519	SLeX	516:519	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	glycans					477:483	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	X					513:513	Sialyl lewis X	500:513	Sialyl lewis X (SLeX)	500:520	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	Tn					533:534	Sialyl Tn	526:534	Sialyl Tn (STn)	526:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	91	dep	glycans	477:483	arg1	X					491:491	Lewis X	485:491	Lewis X (LeX)	485:497	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	1	92	theme	murine	100:105	arg1	lines					112:116	murine cell lines	100:116	murine cell lines	100:116	The characterization of murine cell lines is of great importance in order to identify preclinical models that could resemble human diseases.
30867744	10	93	from	expression	1656:1665	arg1	lines					1682:1686	cancer cell lines	1670:1686	cancer cell lines	1670:1686	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	10	93	from	expression	1656:1665	arg1	tumors					1700:1705	patient tumors	1692:1705	patient tumors	1692:1705	The assessment of its expression in cancer cell lines and patient tumors is of great importance, considering the relevance of this ganglioside in tumor biology.
30867744	11	94	theme	models	2004:2009	arg1	characterization					1970:1985	the characterization	1966:1985	the characterization of murine cancer models	1966:2009	The data obtained by the present study demonstrates that glycan expression may be substantially altered depending on the growth conditions, highlighting the importance of the characterization of murine cancer models.
30867744	3	95	theme	models	465:470	arg1	development					423:433	the development	419:433	the development of preclinical bladder cancer models	419:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	5	96	theme	NGcGM3	1054:1059	arg1	expression					1021:1030	the expression	1017:1030	the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells	1017:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	6	97	dep	line	1287:1290	arg1	either					1275:1280	either	1275:1280	either	1275:1280	The expression of LeX was not detected in either cell line, whereas SLeX was expressed in monolayers, spheroids and orthotopic tumors of both cell lines.
30867744	3	98	theme	available	405:413	arg1	MB49-I					353:358	MB49-I	353:358	MB49-I	353:358	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	98	theme	available	405:413	arg1	lines					399:403	the only two murine cell lines	374:403	the only two murine cell lines available for the development of preclinical bladder cancer models	374:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	98	theme	available	405:413	arg1	MB49					344:347	MB49	344:347	MB49	344:347	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	4	99	theme	Sialyl	500:505	arg1	glycans					477:483	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn)	473:540	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	99	theme	Sialyl	500:505	arg1	SLeX					516:519	SLeX	516:519	SLeX	516:519	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	4	99	theme	Sialyl	500:505	arg1	X					513:513	Sialyl lewis X	500:513	Sialyl lewis X (SLeX)	500:520	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	3	100	theme	bladder	450:456	arg1	models					465:470	preclinical bladder cancer models	438:470	preclinical bladder cancer models	438:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	0	101	theme	tumor	58:62	arg1	lines					69:73	murine tumor cell lines	51:73	murine tumor cell lines	51:73	Expression of bladder cancer-associated glycans in murine tumor cell lines.
30867744	5	102	theme	LeX	1035:1037	arg1	expression					1021:1030	the expression	1017:1030	the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells	1017:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	3	103	theme	cell	394:397	arg1	MB49-I					353:358	MB49-I	353:358	MB49-I	353:358	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	103	theme	cell	394:397	arg1	lines					399:403	the only two murine cell lines	374:403	the only two murine cell lines available for the development of preclinical bladder cancer models	374:470	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	3	103	theme	cell	394:397	arg1	MB49					344:347	MB49	344:347	MB49	344:347	MB49 and MB49-I are currently the only two murine cell lines available for the development of preclinical bladder cancer models.
30867744	2	104	theme	structures	332:341	arg1	expression					278:287	excessive or novel expression	259:287	excessive or novel expression of glycans	259:298	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	2	104	theme	structures	332:341	arg1	loss					253:256	the loss	249:256	the loss	249:256	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	2	104	theme	structures	332:341	arg1	appearance					308:317	the appearance	304:317	the appearance of truncated structures	304:341	Aberrant glycosylation includes the loss, excessive or novel expression of glycans and the appearance of truncated structures.
30867744	8	105	theme	human	1505:1509	arg1	cancer					1529:1534	human or murine bladder cancer	1505:1534	human or murine bladder cancer	1505:1534	There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer.
30867744	5	106	theme	SLeX	1040:1043	arg1	expression					1021:1030	the expression	1017:1030	the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells	1017:1084	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	4	107	theme	many	720:723	arg1	types					725:729	many types	720:729	many types of tumors	720:739	The glycans Lewis X (LeX), Sialyl lewis X (SLeX) and Sialyl Tn (STn) have previously been associated with aggressiveness, dissemination and poor prognosis in human bladder cancer, additionally N-glycolyl GM3 (NGcGM3) is a neo-antigen expressed in many types of tumors; however, to the best of our knowledge, its expression has not previously been assessed in this type of cancer.
30867744	8	108	theme	murine	1514:1519	arg1	cancer					1529:1534	human or murine bladder cancer	1505:1534	human or murine bladder cancer	1505:1534	There are no reports concerning the expression of NGcGM3 in human or murine bladder cancer.
30867744	5	109	theme	different	1090:1098	arg1	cultures					1136:1143	monolayer cultures	1126:1143	monolayer cultures	1126:1143	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	109	theme	different	1090:1098	arg1	tumors					1225:1230	mouse heterotopic and orthotopic tumors	1192:1230	mouse heterotopic and orthotopic tumors	1192:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	109	theme	different	1090:1098	arg1	conditions					1107:1116	different growth conditions	1090:1116	different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors	1090:1230	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
30867744	5	109	theme	different	1090:1098	arg1	spheroids					1178:1186	three-dimensional multicellular spheroids	1146:1186	three-dimensional multicellular spheroids	1146:1186	Taking into account the relevance of glycans in tumor biology and considering that they can act as targets of therapies and biomarkers, the present study evaluated the expression of LeX, SLeX, STn and NGcGM3 in MB49 and MB49-I cells, in different growth conditions such as monolayer cultures, three-dimensional multicellular spheroids and mouse heterotopic and orthotopic tumors.
29384543	12	0	theme	microbial	1668:1676	arg1	composition					1678:1688	microbial composition	1668:1688	microbial composition	1668:1688	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	13	1	theme	intestinal	1853:1862	arg1	function					1872:1879	the intestinal barrier function	1849:1879	the intestinal barrier function	1849:1879	In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function.
29384543	3	2	theme	model	481:485	arg1	group					487:491	the model group	477:491	the model group	477:491	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	12	3	theme	intestinal	1623:1632	arg1	microbiota					1634:1643	the intestinal microbiota	1619:1643	the intestinal microbiota	1619:1643	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	2	4	from	function	315:322	arg1	rats					327:330	rats	327:330	rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	327:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	11	5	theme	expression	1414:1423	arg1	levels					1425:1430	the expression levels	1410:1430	the expression levels of phosphorylated p38 MAPK and ERK1/2	1410:1468	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	5	6	theme	d-lactic	796:803	arg1	d-LA					811:814	d-LA	811:814	d-LA	811:814	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	5	6	theme	d-lactic	796:803	arg1	acid					805:808	d-lactic acid	796:808	d-lactic acid (d-LA)	796:815	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	14	7	theme	breast	1983:1988	arg1	cancer					1990:1995	breast cancer	1983:1995	breast cancer	1983:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	7	8	theme	intestinal	1047:1056	arg1	wall					1058:1061	the intestinal wall	1043:1061	the intestinal wall of the model group	1043:1080	Our results suggested that the intestinal wall of the model group was damaged.
29384543	4	9	theme	histopathological	630:646	arg1	analysis					648:655	Intestinal histopathological analysis	619:655	Intestinal histopathological analysis	619:655	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	1	10	theme	Recent	103:108	arg1	studies					119:125	Recent research studies	103:125	Recent research studies	103:125	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	2	11	from	effect	253:258	arg1	function					315:322	intestinal barrier function	296:322	intestinal barrier function	296:322	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	2	11	from	effect	253:258	arg1	flora					286:290	intestinal flora	275:290	intestinal flora	275:290	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	3	12	theme	F1	502:503	arg1	groups					512:517	the F1 and F2 groups	498:517	the F1 and F2 groups	498:517	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	6	13	theme	junction	919:926	arg1	proteins					933:940	the tight junction (TJ) proteins	909:940	the tight junction (TJ) proteins	909:940	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	6	13	theme	junction	919:926	arg1	ERK1/2					971:976	ERK1/2	971:976	ERK1/2	971:976	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	6	13	theme	junction	919:926	arg1	MAPK					962:965	phosphorylated p38 MAPK	943:965	phosphorylated p38 MAPK	943:965	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	13	14	theme	microbiota	1815:1824	arg1	composition					1826:1836	the fecal microbiota composition	1805:1836	the fecal microbiota composition	1805:1836	In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function.
29384543	0	15	theme	intestinal	47:56	arg1	barrier					58:64	intestinal barrier	47:64	intestinal barrier	47:64	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	12	16	theme	rDNA	1536:1539	arg1	sequencing					1557:1566	16S rDNA high-throughput sequencing	1532:1566	16S rDNA high-throughput sequencing	1532:1566	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	5	17	theme	diamine	818:824	arg1	DAO					835:837	DAO	835:837	DAO	835:837	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	5	17	theme	diamine	818:824	arg1	oxidase					826:832	diamine oxidase	818:832	diamine oxidase (DAO)	818:838	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	12	18	theme	sequencing	1557:1566	arg1	results					1521:1527	The results	1517:1527	The results of 16S rDNA high-throughput sequencing	1517:1566	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	14	19	theme	fucoidan	1913:1920	arg1	use					1906:1908	the use	1902:1908	the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer	1902:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	3	20	theme	Sprague-Dawley	412:425	arg1	rats					427:430	Sixty female Sprague-Dawley rats	399:430	Sixty female Sprague-Dawley rats	399:430	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	7	21	theme	group	1076:1080	arg1	wall					1058:1061	the intestinal wall	1043:1061	the intestinal wall of the model group	1043:1080	Our results suggested that the intestinal wall of the model group was damaged.
29384543	14	22	theme	cancer	1990:1995	arg1	prevention					1969:1978	potential prevention	1959:1978	potential prevention of breast cancer	1959:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	9	23	theme	endotoxin	1207:1215	arg1	levels					1190:1195	the levels	1186:1195	the levels of plasma endotoxin, d-LA and DAO	1186:1229	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	5	24	theme	endotoxin	844:852	arg1	contents					784:791	The contents	780:791	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma	780:862	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	6	25	theme	phosphorylated	943:956	arg1	proteins					933:940	the tight junction (TJ) proteins	909:940	the tight junction (TJ) proteins	909:940	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	6	25	theme	phosphorylated	943:956	arg1	MAPK					962:965	phosphorylated p38 MAPK	943:965	phosphorylated p38 MAPK	943:965	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	10	26	theme	ZO-1	1356:1359	arg1	expressions					1341:1351	the expressions	1337:1351	the expressions of ZO-1, occludin, claudin-1 and claudin-8	1337:1394	Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8.
29384543	4	27	theme	rDNA	679:682	arg1	sequencing					700:709	16S rDNA high-throughput sequencing	675:709	16S rDNA high-throughput sequencing	675:709	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	9	28	theme	d-LA	1218:1221	arg1	levels					1190:1195	the levels	1186:1195	the levels of plasma endotoxin, d-LA and DAO	1186:1229	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	6	29	theme	proteins	933:940	arg1	levels					899:904	Expression levels	888:904	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2	888:976	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	14	30	theme	potential	1959:1967	arg1	prevention					1969:1978	potential prevention	1959:1978	potential prevention of breast cancer	1959:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	2	31	theme	breast	383:388	arg1	cancers					390:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	9	32	theme	DAO	1227:1229	arg1	levels					1190:1195	the levels	1186:1195	the levels of plasma endotoxin, d-LA and DAO	1186:1229	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	10	33	theme	claudin-1	1372:1380	arg1	expressions					1341:1351	the expressions	1337:1351	the expressions of ZO-1, occludin, claudin-1 and claudin-8	1337:1394	Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8.
29384543	3	34	theme	mg	577:578	arg1	concentrations					547:560	concentrations	547:560	concentrations of 200 and 400 mg per kg bw (body weight), respectively	547:616	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	4	35	theme	flora	761:765	arg1	composition					767:777	the intestinal flora composition	746:777	the intestinal flora composition	746:777	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	11	36	theme	MAPK	1454:1457	arg1	levels					1425:1430	the expression levels	1410:1430	the expression levels of phosphorylated p38 MAPK and ERK1/2	1410:1468	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	12	37	theme	Bacteroidetes/Firmicutes	1710:1733	arg1	ratio					1742:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	12	38	theme	increased	1700:1708	arg1	ratio					1742:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	10	39	theme	occludin	1362:1369	arg1	expressions					1341:1351	the expressions	1337:1351	the expressions of ZO-1, occludin, claudin-1 and claudin-8	1337:1394	Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8.
29384543	1	40	dep	occurrence	183:192	arg1	the					179:181	the	179:181	the	179:181	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	11	41	theme	phosphorylated	1435:1448	arg1	MAPK					1454:1457	phosphorylated p38 MAPK	1435:1457	phosphorylated p38 MAPK	1435:1457	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	2	42	theme	barrier	307:313	arg1	function					315:322	intestinal barrier function	296:322	intestinal barrier function	296:322	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	4	43	used	used	715:718	arg2	sequencing					700:709	16S rDNA high-throughput sequencing	675:709	16S rDNA high-throughput sequencing	675:709	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	9	44	theme	F1	1248:1249	arg1	groups					1258:1263	the F1 and F2 groups	1244:1263	the F1 and F2 groups	1244:1263	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	6	45	theme	western	998:1004	arg1	blotting					1006:1013	western blotting	998:1013	western blotting	998:1013	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	2	46	theme	-induced	374:381	arg1	cancers					390:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	9	47	theme	model	1290:1294	arg1	group					1296:1300	the model group	1286:1300	the model group	1286:1300	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	9	48	theme	F2	1255:1256	arg1	groups					1258:1263	the F1 and F2 groups	1244:1263	the F1 and F2 groups	1244:1263	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	10	49	theme	claudin-8	1386:1394	arg1	expressions					1341:1351	the expressions	1337:1351	the expressions of ZO-1, occludin, claudin-1 and claudin-8	1337:1394	Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8.
29384543	11	50	theme	ERK1/2	1463:1468	arg1	levels					1425:1430	the expression levels	1410:1430	the expression levels of phosphorylated p38 MAPK and ERK1/2	1410:1468	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	14	51	theme	intestinal	1928:1937	arg1	modulator					1945:1953	an intestinal flora modulator	1925:1953	an intestinal flora modulator for potential prevention of breast cancer	1925:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	12	52	theme	microbiota	1634:1643	arg1	diversity					1606:1614	the diversity	1602:1614	the diversity of the intestinal microbiota	1602:1643	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	12	53	from	changes	1657:1663	arg1	composition					1678:1688	microbial composition	1668:1688	microbial composition	1668:1688	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	11	54	theme	fucoidan	1490:1497	arg1	groups					1509:1514	fucoidan treatment groups	1490:1514	fucoidan treatment groups	1490:1514	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	13	55	theme	barrier	1864:1870	arg1	function					1872:1879	the intestinal barrier function	1849:1879	the intestinal barrier function	1849:1879	In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function.
29384543	2	56	theme	fucoidan	263:270	arg1	effect					253:258	the effect	249:258	the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	249:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	1	57	theme	intestinal	147:156	arg1	related					168:174	related	168:174	related	168:174	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	1	57	theme	intestinal	147:156	arg1	flora					158:162	the intestinal flora	143:162	the intestinal flora	143:162	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	4	58	theme	composition	767:777	arg1	overview					734:741	an overview	731:741	an overview of the intestinal flora composition	731:777	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	8	59	theme	fucoidan	1110:1117	arg1	intervention					1119:1130	fucoidan intervention	1110:1130	fucoidan intervention	1110:1130	However, after fucoidan intervention, the villi were gradually restored.
29384543	5	60	theme	acid	805:808	arg1	contents					784:791	The contents	780:791	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma	780:862	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	0	61	with	rats	78:81	arg1	cancer					95:100	breast cancer	88:100	breast cancer	88:100	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	2	62	theme	intestinal	275:284	arg1	flora					286:290	intestinal flora	275:290	intestinal flora	275:290	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	5	63	from	contents	784:791	arg1	plasma					857:862	plasma	857:862	plasma	857:862	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	5	64	theme	oxidase	826:832	arg1	contents					784:791	The contents	780:791	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma	780:862	The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA.
29384543	4	65	theme	Intestinal	619:628	arg1	analysis					648:655	Intestinal histopathological analysis	619:655	Intestinal histopathological analysis	619:655	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	3	66	theme	200	565:567	arg1	mg					577:578	200 and 400 mg	565:578	mg	577:578	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	1	67	theme	research	110:117	arg1	studies					119:125	Recent research studies	103:125	Recent research studies	103:125	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	3	68	theme	F2	509:510	arg1	groups					512:517	the F1 and F2 groups	498:517	the F1 and F2 groups	498:517	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	13	69	theme	fecal	1809:1813	arg1	composition					1826:1836	the fecal microbiota composition	1805:1836	the fecal microbiota composition	1805:1836	In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function.
29384543	12	70	theme	high-throughput	1541:1555	arg1	sequencing					1557:1566	16S rDNA high-throughput sequencing	1532:1566	16S rDNA high-throughput sequencing	1532:1566	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	6	71	theme	tight	913:917	arg1	TJ					929:930	TJ	929:930	TJ	929:930	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	6	71	theme	tight	913:917	arg1	junction					919:926	tight junction	913:926	the tight junction (TJ) proteins	909:940	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	12	72	theme	16S	1532:1534	arg1	sequencing					1557:1566	16S rDNA high-throughput sequencing	1532:1566	16S rDNA high-throughput sequencing	1532:1566	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	0	73	from	effect	4:9	arg1	flora					37:41	intestinal flora	26:41	intestinal flora	26:41	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	0	73	from	effect	4:9	arg1	barrier					58:64	intestinal barrier	47:64	intestinal barrier	47:64	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	13	74	theme	fucoidan	1782:1789	arg1	supplement					1768:1777	the supplement	1764:1777	the supplement of fucoidan	1764:1789	In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function.
29384543	2	75	from	flora	286:290	arg1	rats					327:330	rats	327:330	rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	327:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	6	76	theme	Expression	888:897	arg1	levels					899:904	Expression levels	888:904	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2	888:976	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	4	77	theme	high-throughput	684:698	arg1	sequencing					700:709	16S rDNA high-throughput sequencing	675:709	16S rDNA high-throughput sequencing	675:709	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	2	78	theme	intestinal	296:305	arg1	function					315:322	intestinal barrier function	296:322	intestinal barrier function	296:322	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	7	79	theme	model	1070:1074	arg1	group					1076:1080	the model group	1066:1080	the model group	1066:1080	Our results suggested that the intestinal wall of the model group was damaged.
29384543	9	80	theme	plasma	1200:1205	arg1	endotoxin					1207:1215	plasma endotoxin	1200:1215	plasma endotoxin	1200:1215	ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group.
29384543	6	81	theme	p38	958:960	arg1	proteins					933:940	the tight junction (TJ) proteins	909:940	the tight junction (TJ) proteins	909:940	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	6	81	theme	p38	958:960	arg1	MAPK					962:965	phosphorylated p38 MAPK	943:965	phosphorylated p38 MAPK	943:965	Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting.
29384543	10	82	theme	Fucoidan	1303:1310	arg1	treatment					1312:1320	Fucoidan treatment	1303:1320	Fucoidan treatment	1303:1320	Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8.
29384543	3	83	theme	female	405:410	arg1	rats					427:430	Sixty female Sprague-Dawley rats	399:430	Sixty female Sprague-Dawley rats	399:430	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	0	84	theme	fucoidan	14:21	arg1	effect					4:9	The effect	0:9	The effect of fucoidan on intestinal flora and intestinal barrier	0:64	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	4	85	theme	16S	675:677	arg1	sequencing					700:709	16S rDNA high-throughput sequencing	675:709	16S rDNA high-throughput sequencing	675:709	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	0	86	theme	intestinal	26:35	arg1	flora					37:41	intestinal flora	26:41	intestinal flora	26:41	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	3	87	theme	400	573:575	arg1	mg					577:578	200 and 400 mg	565:578	mg	577:578	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	1	88	theme	breast	210:215	arg1	cancer					217:222	breast cancer	210:222	breast cancer	210:222	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	11	89	theme	p38	1450:1452	arg1	MAPK					1454:1457	phosphorylated p38 MAPK	1435:1457	phosphorylated p38 MAPK	1435:1457	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	0	90	theme	breast	88:93	arg1	cancer					95:100	breast cancer	88:100	breast cancer	88:100	The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer.
29384543	12	91	theme	phylum	1735:1740	arg1	ratio					1742:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	the increased Bacteroidetes/Firmicutes phylum ratio	1696:1746	The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio.
29384543	14	92	theme	flora	1939:1943	arg1	modulator					1945:1953	an intestinal flora modulator	1925:1953	an intestinal flora modulator for potential prevention of breast cancer	1925:1995	The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.
29384543	3	93	theme	body	591:594	arg1	bw					587:588	kg bw	584:588	kg bw (body weight)	584:602	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	3	93	theme	body	591:594	arg1	weight					596:601	body weight	591:601	body weight	591:601	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	4	94	theme	intestinal	750:759	arg1	composition					767:777	the intestinal flora composition	746:777	the intestinal flora composition	746:777	Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition.
29384543	2	95	with	rats	327:330	arg1	cancers					390:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers	337:396	This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers.
29384543	3	96	theme	control	462:468	arg1	group					470:474	the control group	458:474	the control group	458:474	Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively.
29384543	11	97	theme	treatment	1499:1507	arg1	groups					1509:1514	fucoidan treatment groups	1490:1514	fucoidan treatment groups	1490:1514	Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups.
29384543	1	98	theme	cancer	217:222	arg1	progress					198:205	progress	198:205	progress	198:205	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
29384543	1	98	theme	cancer	217:222	arg1	occurrence					183:192	occurrence	183:192	occurrence	183:192	Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer.
30365990	1	0	theme	hydroxyethyl	159:170	arg1	HEC					183:185	HEC	183:185	HEC	183:185	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	1	0	theme	hydroxyethyl	159:170	arg1	cellulose					172:180	hydroxyethyl cellulose	159:180	hydroxyethyl cellulose (HEC)	159:186	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	7	1	theme	TGA	771:773	arg1	results					775:781	The FTIR and TGA results	758:781	The FTIR and TGA results	758:781	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	10	2	theme	human	1095:1099	arg1	cells					1118:1122	human fibroblast (hFB) cells	1095:1122	human fibroblast (hFB) cells	1095:1122	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	11	3	theme	HEC/PVA	1267:1273	arg1	scaffolds					1275:1283	HEC/PVA scaffolds	1267:1283	HEC/PVA scaffolds fabricated by freeze-drying method	1267:1318	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	6	4	theme	degradation	702:712	arg1	rate					714:717	low degradation rate	698:717	low degradation rate	698:717	After 7 days, all blended scaffolds showed low degradation rate with the increased of PVA composition.
30365990	1	5	theme	improved	224:231	arg1	profiles					251:258	improved cellular adhesion profiles	224:258	improved cellular adhesion profiles	224:258	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	5	6	theme	PVA	616:618	arg1	cooperation					620:630	PVA cooperation	616:630	PVA cooperation in the polymer matrix	616:652	The water uptake of HEC was controlled by PVA cooperation in the polymer matrix.
30365990	8	7	theme	HEC	913:915	arg1	range					904:908	range	904:908	range of HEC and PVA	904:923	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	6	8	theme	low	698:700	arg1	rate					714:717	low degradation rate	698:717	low degradation rate	698:717	After 7 days, all blended scaffolds showed low degradation rate with the increased of PVA composition.
30365990	7	9	theme	FTIR	762:765	arg1	results					775:781	The FTIR and TGA results	758:781	The FTIR and TGA results	758:781	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	1	10	theme	cellular	233:240	arg1	profiles					251:258	improved cellular adhesion profiles	224:258	improved cellular adhesion profiles	224:258	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	3	11	from	diameter	459:466	arg1	range					473:477	a range	471:477	a range of both pure HEC (2-20 μm)	471:504	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	3	12	theme	scaffolds	442:450	arg1	size					416:419	Pore size	411:419	Pore size of HEC/PVA (2-40 μm) scaffolds	411:450	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	10	13	theme	cell	1164:1167	arg1	proliferation					1169:1181	better cell proliferation	1157:1181	better cell proliferation observed after 7 days of cultivation	1157:1218	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	1	14	theme	adhesion	242:249	arg1	profiles					251:258	improved cellular adhesion profiles	224:258	improved cellular adhesion profiles	224:258	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	9	15	theme	Higher	980:985	arg1	modulus					995:1001	Higher Young's modulus	980:1001	Higher Young's modulus	980:1001	Higher Young's modulus was obtained with the increasing of HEC value.
30365990	8	16	theme	PVA	921:923	arg1	range					904:908	range	904:908	range of HEC and PVA	904:923	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	7	17	theme	blended	840:846	arg1	scaffolds					848:856	blended scaffolds	840:856	blended scaffolds	840:856	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	10	18	theme	better	1157:1162	arg1	proliferation					1169:1181	better cell proliferation	1157:1181	better cell proliferation observed after 7 days of cultivation	1157:1218	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	9	19	theme	HEC	1039:1041	arg1	value					1043:1047	HEC value	1039:1047	HEC value	1039:1047	Higher Young's modulus was obtained with the increasing of HEC value.
30365990	11	20	theme	freeze-drying	1299:1311	arg1	method					1313:1318	freeze-drying method	1299:1318	freeze-drying method	1299:1318	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	6	21	theme	blended	673:679	arg1	scaffolds					681:689	all blended scaffolds	669:689	all blended scaffolds	669:689	After 7 days, all blended scaffolds showed low degradation rate with the increased of PVA composition.
30365990	11	22	theme	tissue	1347:1352	arg1	applications					1366:1377	skin tissue engineering applications	1342:1377	skin tissue engineering applications	1342:1377	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	11	23	theme	scaffolds	1275:1283	arg1	biocompatible					1250:1262	biocompatible	1250:1262	biocompatible	1250:1262	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	2	24	theme	HEC	294:296	arg1	combination					279:289	The combination	275:289	The combination of HEC and PVA	275:304	The combination of HEC and PVA were synthesized using freeze-drying technique and characterized using SEM, ATR-FTIR, TGA, DSC, and UTM.
30365990	5	25	theme	water	578:582	arg1	uptake					584:589	The water uptake	574:589	The water uptake of HEC	574:596	The water uptake of HEC was controlled by PVA cooperation in the polymer matrix.
30365990	8	26	theme	polymers	970:977	arg1	miscibility					941:951	the miscibility	937:951	the miscibility of HEC/PVA blend polymers	937:977	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	0	27	theme	hydroxyethyl	17:28	arg1	cellulose					30:38	hydroxyethyl cellulose	17:38	hydroxyethyl cellulose	17:38	Highly porous of hydroxyethyl cellulose biocomposite scaffolds for tissue engineering.
30365990	5	28	theme	HEC	594:596	arg1	uptake					584:589	The water uptake	574:589	The water uptake of HEC	574:596	The water uptake of HEC was controlled by PVA cooperation in the polymer matrix.
30365990	8	29	theme	DSC	890:892	arg1	values					880:885	The Tg values	873:885	The Tg values of DSC curved in range of HEC and PVA	873:923	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	7	30	theme	scaffolds	848:856	arg1	interactions					810:821	possible chemical interactions	792:821	possible chemical interactions	792:821	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	7	30	theme	scaffolds	848:856	arg1	loss					832:835	mass loss	827:835	mass loss of blended scaffolds	827:856	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	3	31	theme	HEC	492:494	arg1	range					473:477	a range	471:477	a range of both pure HEC (2-20 μm)	471:504	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	3	32	from	PVA	510:512	arg1	range					473:477	a range	471:477	a range of both pure HEC (2-20 μm)	471:504	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	11	33	theme	engineering	1354:1364	arg1	applications					1366:1377	skin tissue engineering applications	1342:1377	skin tissue engineering applications	1342:1377	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	11	34	theme	skin	1342:1345	arg1	applications					1366:1377	skin tissue engineering applications	1342:1377	skin tissue engineering applications	1342:1377	These results suggested that biocompatible of HEC/PVA scaffolds fabricated by freeze-drying method might be suitable for skin tissue engineering applications.
30365990	8	35	theme	blend	964:968	arg1	polymers					970:977	HEC/PVA blend polymers	956:977	HEC/PVA blend polymers	956:977	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	9	36	theme	Young	987:991	arg1	modulus					995:1001	Higher Young's modulus	980:1001	Higher Young's modulus	980:1001	Higher Young's modulus was obtained with the increasing of HEC value.
30365990	1	37	theme	vinyl	198:202	arg1	poly					192:195	poly	192:195	poly (vinyl alcohol) (PVA)	192:217	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	1	37	theme	vinyl	198:202	arg1	alcohol					204:210	vinyl alcohol	198:210	vinyl alcohol	198:210	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	0	38	theme	cellulose	30:38	arg1	porous					7:12	Highly porous	0:12	Highly porous of hydroxyethyl cellulose	0:38	Highly porous of hydroxyethyl cellulose biocomposite scaffolds for tissue engineering.
30365990	8	39	theme	Tg	877:878	arg1	values					880:885	The Tg values	873:885	The Tg values of DSC curved in range of HEC and PVA	873:923	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	8	40	theme	HEC/PVA	956:962	arg1	polymers					970:977	HEC/PVA blend polymers	956:977	HEC/PVA blend polymers	956:977	The Tg values of DSC curved in range of HEC and PVA represented the miscibility of HEC/PVA blend polymers.
30365990	6	41	theme	PVA	741:743	arg1	composition					745:755	PVA composition	741:755	PVA composition	741:755	After 7 days, all blended scaffolds showed low degradation rate with the increased of PVA composition.
30365990	4	42	theme	high	549:552	arg1	porosity					554:561	high porosity	549:561	high porosity above 85%	549:571	All scaffolds revealed high porosity above 85%.
30365990	10	43	with	adhered	1124:1130	arg1	proliferation					1169:1181	better cell proliferation	1157:1181	better cell proliferation observed after 7 days of cultivation	1157:1218	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	5	44	from	cooperation	620:630	arg1	matrix					647:652	the polymer matrix	635:652	the polymer matrix	635:652	The water uptake of HEC was controlled by PVA cooperation in the polymer matrix.
30365990	3	45	theme	2-40 μm	433:439	arg1	scaffolds					442:450	HEC/PVA (2-40 μm) scaffolds	424:450	HEC/PVA (2-40 μm) scaffolds	424:450	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	3	46	theme	HEC/PVA	424:430	arg1	scaffolds					442:450	HEC/PVA (2-40 μm) scaffolds	424:450	HEC/PVA (2-40 μm) scaffolds	424:450	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	10	47	theme	cultivation	1208:1218	arg1	7 days					1198:1203	7 days	1198:1203	7 days of cultivation	1198:1218	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	7	48	theme	possible	792:799	arg1	interactions					810:821	possible chemical interactions	792:821	possible chemical interactions	792:821	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	7	49	theme	mass	827:830	arg1	loss					832:835	mass loss	827:835	mass loss of blended scaffolds	827:856	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	10	50	theme	hFB	1113:1115	arg1	cells					1118:1122	human fibroblast (hFB) cells	1095:1122	human fibroblast (hFB) cells	1095:1122	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	1	51	theme	porous	123:128	arg1	scaffold					144:151	a porous biocompatible scaffold	121:151	a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability	121:272	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	7	52	theme	chemical	801:808	arg1	interactions					810:821	possible chemical interactions	792:821	possible chemical interactions	792:821	The FTIR and TGA results explicit possible chemical interactions and mass loss of blended scaffolds, respectively.
30365990	10	53	theme	Cell-scaffolds	1050:1063	arg1	interaction					1065:1075	Cell-scaffolds interaction	1050:1075	Cell-scaffolds interaction	1050:1075	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	1	54	theme	biocompatible	130:142	arg1	scaffold					144:151	a porous biocompatible scaffold	121:151	a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability	121:272	This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability.
30365990	2	55	theme	PVA	302:304	arg1	combination					279:289	The combination	275:289	The combination of HEC and PVA	275:304	The combination of HEC and PVA were synthesized using freeze-drying technique and characterized using SEM, ATR-FTIR, TGA, DSC, and UTM.
30365990	5	56	theme	polymer	639:645	arg1	matrix					647:652	the polymer matrix	635:652	the polymer matrix	635:652	The water uptake of HEC was controlled by PVA cooperation in the polymer matrix.
30365990	3	57	theme	Pore	411:414	arg1	size					416:419	Pore size	411:419	Pore size of HEC/PVA (2-40 μm) scaffolds	411:450	Pore size of HEC/PVA (2-40 μm) scaffolds showed diameter in a range of both pure HEC (2-20 μm) and PVA (14-70 μm).
30365990	2	58	theme	freeze-drying	329:341	arg1	technique					343:351	freeze-drying technique	329:351	freeze-drying technique	329:351	The combination of HEC and PVA were synthesized using freeze-drying technique and characterized using SEM, ATR-FTIR, TGA, DSC, and UTM.
30365990	10	59	theme	fibroblast	1101:1110	arg1	cells					1118:1122	human fibroblast (hFB) cells	1095:1122	human fibroblast (hFB) cells	1095:1122	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
30365990	0	60	theme	tissue	67:72	arg1	engineering					74:84	tissue engineering	67:84	tissue engineering	67:84	Highly porous of hydroxyethyl cellulose biocomposite scaffolds for tissue engineering.
30365990	10	61	theme	polymer	1135:1141	arg1	matrices					1143:1150	polymer matrices	1135:1150	polymer matrices	1135:1150	Cell-scaffolds interaction demonstrated that human fibroblast (hFB) cells adhered to polymer matrices with better cell proliferation observed after 7 days of cultivation.
31881739	5	0	theme	nanocomposite	816:828	arg1	properties					798:807	the properties	794:807	the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP	794:890	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	9	1	theme	chitosan	1662:1669	arg1	fibers					1671:1676	the chitosan fibers	1658:1676	the chitosan fibers	1658:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	3	2	theme	CS/silica	514:522	arg1	nanofibers					524:533	the CS/silica nanofibers	510:533	the CS/silica nanofibers	510:533	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	9	3	from	size	1585:1588	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	6	4	dep	aureus	976:981	arg1	aureus					987:992	S. aureus	984:992	S. aureus	984:992	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	6	4	dep	aureus	976:981	arg1	coli					1030:1033	E. coli	1027:1033	E. coli	1027:1033	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	9	5	from	characteristics	1639:1653	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	6	6	theme	aureus	976:981	arg1	ATCC25922					1036:1044	Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	961:1044	Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	961:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	9	7	theme	fibers	1671:1676	arg1	characteristics					1639:1653	the characteristics	1635:1653	the characteristics of the chitosan fibers	1635:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	7	theme	fibers	1671:1676	arg1	properties					1555:1564	the properties	1551:1564	the properties of composites	1551:1578	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	7	theme	fibers	1671:1676	arg1	size					1585:1588	size	1585:1588	size	1585:1588	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	7	theme	fibers	1671:1676	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	7	theme	fibers	1671:1676	arg1	morphology					1472:1481	The morphology	1468:1481	The morphology of the AgNP in solution, both initial and extracted from composite	1468:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	8	from	properties	1555:1564	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	2	9	theme	nanofiber	356:364	arg1	scaffolds					366:374	highly porous 3D nanofiber scaffolds	339:374	highly porous 3D nanofiber scaffolds	339:374	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	9	10	dep	size	1585:1588	arg1	the					1581:1583	the	1581:1583	the	1581:1583	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	3	11	theme	chitosan	563:570	arg1	nanofibers					572:581	chitosan nanofibers	563:581	chitosan nanofibers	563:581	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	3	12	theme	silica	588:593	arg1	phase					595:599	silica phase	588:599	silica phase decorated by silver nanoparticles (Ag/CS/silica)	588:648	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	3	13	theme	Silver	377:382	arg1	nanoparticles					384:396	Silver nanoparticles	377:396	Silver nanoparticles in the form of a well-dispersed metallic phase	377:443	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	3	14	from	nanoparticles	384:396	arg1	form					405:408	the form	401:408	the form of a well-dispersed metallic phase	401:443	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	1	15	with	materials	154:162	arg1	dispersion					202:211	even silver nanoparticles (AgNP) dispersion	169:211	even silver nanoparticles (AgNP) dispersion	169:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	9	16	dep	AgNP	1490:1493	arg1	both					1508:1511	both	1508:1511	both	1508:1511	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	16	dep	AgNP	1490:1493	arg1	extracted					1525:1533	extracted	1525:1533	extracted from composite	1525:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	8	17	theme	X-ray	1408:1412	arg1	XRD					1427:1429	XRD	1427:1429	XRD	1427:1429	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	17	theme	X-ray	1408:1412	arg1	diffraction					1414:1424	X-ray diffraction	1408:1424	X-ray diffraction (XRD)	1408:1430	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	7	18	from	techniques	1151:1160	arg1	characterization					1219:1234	the characterization	1215:1234	the characterization of this type of nanocomposite	1215:1264	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	4	19	theme	Gram-negative	739:751	arg1	bacteria					753:760	Gram-positive and Gram-negative bacteria	721:760	Gram-positive and Gram-negative bacteria	721:760	The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria.
31881739	6	20	theme	AgNP	948:951	arg1	MIC					931:933	MIC	931:933	MIC	931:933	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	6	20	theme	AgNP	948:951	arg1	concentration					916:928	The minimum inhibitory concentration	893:928	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	893:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	8	21	theme	small	1371:1375	arg1	SAXS					1401:1404	SAXS	1401:1404	SAXS	1401:1404	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	21	theme	small	1371:1375	arg1	scattering					1389:1398	small angle X-ray scattering	1371:1398	small angle X-ray scattering (SAXS)	1371:1405	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	22	theme	atomic	1437:1442	arg1	AFM					1462:1464	AFM	1462:1464	AFM	1462:1464	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	22	theme	atomic	1437:1442	arg1	microscopy					1450:1459	atomic force microscopy	1437:1459	atomic force microscopy (AFM)	1437:1465	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	1	23	theme	silver	174:179	arg1	AgNP					196:199	AgNP	196:199	AgNP	196:199	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	1	23	theme	silver	174:179	arg1	nanoparticles					181:193	even silver nanoparticles	169:193	even silver nanoparticles (AgNP) dispersion	169:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	6	24	theme	obtained	939:946	arg1	AgNP					948:951	obtained AgNP	939:951	obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	939:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	5	25	theme	silver	838:843	arg1	nanoparticles					845:857	silver nanoparticles	838:857	silver nanoparticles	838:857	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	8	26	theme	angle	1377:1381	arg1	SAXS					1401:1404	SAXS	1401:1404	SAXS	1401:1404	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	26	theme	angle	1377:1381	arg1	scattering					1389:1398	small angle X-ray scattering	1371:1398	small angle X-ray scattering (SAXS)	1371:1405	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	9	27	theme	electron	1697:1704	arg1	microscopy					1706:1715	electron microscopy	1697:1715	electron microscopy (SEM and TEM)	1697:1729	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	1	28	theme	nanoparticles	181:193	arg1	dispersion					202:211	even silver nanoparticles (AgNP) dispersion	169:211	even silver nanoparticles (AgNP) dispersion	169:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	5	29	theme	colloidal	865:873	arg1	solution					875:882	a colloidal solution	863:882	a colloidal solution of AgNP	863:890	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	1	30	theme	simple	99:104	arg1	method					134:139	A simple, low-cost, and reproducible method	97:139	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion	97:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	7	31	theme	type	1244:1247	arg1	characterization					1219:1234	the characterization	1215:1234	the characterization of this type of nanocomposite	1215:1264	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	3	32	theme	phase	439:443	arg1	form					405:408	the form	401:408	the form of a well-dispersed metallic phase	401:443	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	5	33	theme	solution	875:882	arg1	properties					798:807	the properties	794:807	the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP	794:890	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	8	34	theme	Ag/CS/silica	1324:1335	arg1	nanocomposites					1308:1321	Ag/CS/silica nanocomposites	1295:1321	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers)	1281:1347	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	34	theme	Ag/CS/silica	1324:1335	arg1	nanofibers					1337:1346	Ag/CS/silica nanofibers	1324:1346	Ag/CS/silica nanofibers	1324:1346	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	7	35	theme	analytical	1140:1149	arg1	techniques					1151:1160	various analytical techniques	1132:1160	various analytical techniques	1132:1160	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	3	36	theme	silver	614:619	arg1	nanoparticles					621:633	silver nanoparticles	614:633	silver nanoparticles (Ag/CS/silica)	614:648	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	3	36	theme	silver	614:619	arg1	Ag/CS/silica					636:647	Ag/CS/silica	636:647	Ag/CS/silica	636:647	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	2	37	theme	electrospinning	303:317	arg1	technique					319:327	an electrospinning technique	300:327	an electrospinning technique to obtain highly porous 3D nanofiber scaffolds	300:374	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	9	38	theme	AgNP	1490:1493	arg1	characteristics					1639:1653	the characteristics	1635:1653	the characteristics of the chitosan fibers	1635:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	38	theme	AgNP	1490:1493	arg1	properties					1555:1564	the properties	1551:1564	the properties of composites	1551:1578	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	38	theme	AgNP	1490:1493	arg1	size					1585:1588	size	1585:1588	size	1585:1588	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	38	theme	AgNP	1490:1493	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	38	theme	AgNP	1490:1493	arg1	morphology					1472:1481	The morphology	1468:1481	The morphology of the AgNP in solution, both initial and extracted from composite	1468:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	3	39	theme	preparation	477:487	arg1	step					489:492	an external preparation step	465:492	an external preparation step	465:492	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	5	40	theme	AgNP	887:890	arg1	solution					875:882	a colloidal solution	863:882	a colloidal solution of AgNP	863:890	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	5	40	theme	AgNP	887:890	arg1	nanocomposite					816:828	the nanocomposite	812:828	the nanocomposite without silver nanoparticles	812:857	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	5	40	theme	AgNP	887:890	arg1	AgNP					887:890	AgNP	887:890	AgNP	887:890	The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP.
31881739	6	41	theme	coli	1021:1024	arg1	ATCC25922					1036:1044	Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	961:1044	Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	961:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	9	42	from	morphology	1472:1481	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	1	43	theme	even	169:172	arg1	AgNP					196:199	AgNP	196:199	AgNP	196:199	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	1	43	theme	even	169:172	arg1	nanoparticles					181:193	even silver nanoparticles	169:193	even silver nanoparticles (AgNP) dispersion	169:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	4	44	theme	antibacterial	655:667	arg1	activity					669:676	The antibacterial activity	651:676	The antibacterial activity of investigated materials	651:702	The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria.
31881739	3	45	theme	well-dispersed	415:428	arg1	phase					439:443	a well-dispersed metallic phase	413:443	a well-dispersed metallic phase	413:443	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	2	46	with	nanofibers	239:248	arg1	phase					262:266	silica phase	255:266	silica phase (CS/silica)	255:278	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	2	46	with	nanofibers	239:248	arg1	CS/silica					269:277	CS/silica	269:277	CS/silica	269:277	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	9	47	dep	microscopy	1706:1715	arg1	TEM					1726:1728	TEM	1726:1728	TEM	1726:1728	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	47	dep	microscopy	1706:1715	arg1	SEM					1718:1720	SEM	1718:1720	SEM	1718:1720	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	48	theme	nanoparticles	1616:1628	arg1	characteristics					1639:1653	the characteristics	1635:1653	the characteristics of the chitosan fibers	1635:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	48	theme	nanoparticles	1616:1628	arg1	properties					1555:1564	the properties	1551:1564	the properties of composites	1551:1578	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	48	theme	nanoparticles	1616:1628	arg1	size					1585:1588	size	1585:1588	size	1585:1588	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	48	theme	nanoparticles	1616:1628	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	48	theme	nanoparticles	1616:1628	arg1	morphology					1472:1481	The morphology	1468:1481	The morphology of the AgNP in solution, both initial and extracted from composite	1468:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	4	49	theme	investigated	681:692	arg1	materials					694:702	investigated materials	681:702	investigated materials	681:702	The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria.
31881739	3	50	theme	metallic	430:437	arg1	phase					439:443	a well-dispersed metallic phase	413:443	a well-dispersed metallic phase	413:443	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	7	51	theme	various	1132:1138	arg1	techniques					1151:1160	various analytical techniques	1132:1160	various analytical techniques	1132:1160	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	8	52	theme	force	1444:1448	arg1	AFM					1462:1464	AFM	1462:1464	AFM	1462:1464	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	52	theme	force	1444:1448	arg1	microscopy					1450:1459	atomic force microscopy	1437:1459	atomic force microscopy (AFM)	1437:1465	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	3	53	theme	external	468:475	arg1	step					489:492	an external preparation step	465:492	an external preparation step	465:492	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	7	54	theme	techniques	1201:1210	arg1	applicability					1178:1190	the applicability	1174:1190	the applicability of these techniques in the characterization of this type of nanocomposite	1174:1264	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	7	54	theme	techniques	1201:1210	arg1	techniques					1151:1160	various analytical techniques	1132:1160	various analytical techniques	1132:1160	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	7	55	from	applicability	1178:1190	arg1	characterization					1219:1234	the characterization	1215:1234	the characterization of this type of nanocomposite	1215:1264	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	7	56	theme	Ag/CS/silica	1102:1113	arg1	nanofibers					1115:1124	Ag/CS/silica nanofibers	1102:1124	Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite,	1102:1265	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	2	57	theme	Chitosan	230:237	arg1	nanofibers					239:248	Chitosan nanofibers	230:248	Chitosan nanofibers with silica phase (CS/silica)	230:278	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	7	58	theme	physicochemical	1066:1080	arg1	characterization					1082:1097	The physicochemical characterization	1062:1097	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite,	1062:1265	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	2	59	theme	3D	353:354	arg1	scaffolds					366:374	highly porous 3D nanofiber scaffolds	339:374	highly porous 3D nanofiber scaffolds	339:374	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	8	60	theme	resulting	1285:1293	arg1	nanocomposites					1308:1321	Ag/CS/silica nanocomposites	1295:1321	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers)	1281:1347	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	60	theme	resulting	1285:1293	arg1	nanofibers					1337:1346	Ag/CS/silica nanofibers	1324:1346	Ag/CS/silica nanofibers	1324:1346	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	7	61	theme	nanofibers	1115:1124	arg1	characterization					1082:1097	The physicochemical characterization	1062:1097	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite,	1062:1265	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	6	62	theme	inhibitory	905:914	arg1	MIC					931:933	MIC	931:933	MIC	931:933	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	6	62	theme	inhibitory	905:914	arg1	concentration					916:928	The minimum inhibitory concentration	893:928	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	893:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	0	63	dep	Nanofibers	40:49	arg1	Characterization					52:67	Characterization	52:67	Characterization	52:67	Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity.
31881739	0	63	dep	Nanofibers	40:49	arg1	Activity					87:94	Antibacterial Activity	73:94	Antibacterial Activity	73:94	Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity.
31881739	9	64	from	solution	1498:1505	arg1	characteristics					1639:1653	the characteristics	1635:1653	the characteristics of the chitosan fibers	1635:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	64	from	solution	1498:1505	arg1	properties					1555:1564	the properties	1551:1564	the properties of composites	1551:1578	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	64	from	solution	1498:1505	arg1	size					1585:1588	size	1585:1588	size	1585:1588	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	64	from	solution	1498:1505	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	64	from	solution	1498:1505	arg1	morphology					1472:1481	The morphology	1468:1481	The morphology of the AgNP in solution, both initial and extracted from composite	1468:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	1	65	theme	reproducible	121:132	arg1	method					134:139	A simple, low-cost, and reproducible method	97:139	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion	97:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	9	66	from	crystallinity	1595:1607	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	6	67	theme	minimum	897:903	arg1	MIC					931:933	MIC	931:933	MIC	931:933	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	6	67	theme	minimum	897:903	arg1	concentration					916:928	The minimum inhibitory concentration	893:928	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922	893:1044	The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined.
31881739	9	68	dep	both	1508:1511	arg1	initial					1513:1519	initial	1513:1519	initial	1513:1519	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	3	69	from	step	489:492	arg1	nanofibers					524:533	the CS/silica nanofibers	510:533	the CS/silica nanofibers	510:533	Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica).
31881739	1	70	theme	low-cost	107:114	arg1	method					134:139	A simple, low-cost, and reproducible method	97:139	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion	97:211	A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established.
31881739	9	71	theme	composites	1569:1578	arg1	characteristics					1639:1653	the characteristics	1635:1653	the characteristics of the chitosan fibers	1635:1676	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	71	theme	composites	1569:1578	arg1	properties					1555:1564	the properties	1551:1564	the properties of composites	1551:1578	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	71	theme	composites	1569:1578	arg1	size					1585:1588	size	1585:1588	size	1585:1588	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	71	theme	composites	1569:1578	arg1	crystallinity					1595:1607	crystallinity	1595:1607	crystallinity	1595:1607	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	71	theme	composites	1569:1578	arg1	morphology					1472:1481	The morphology	1468:1481	The morphology of the AgNP in solution, both initial and extracted from composite	1468:1548	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	9	72	from	AgNP	1490:1493	arg1	solution					1498:1505	solution	1498:1505	solution	1498:1505	The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).
31881739	8	73	theme	X-ray	1383:1387	arg1	SAXS					1401:1404	SAXS	1401:1404	SAXS	1401:1404	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	73	theme	X-ray	1383:1387	arg1	scattering					1389:1398	small angle X-ray scattering	1371:1398	small angle X-ray scattering (SAXS)	1371:1405	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	74	theme	Ag/CS/silica	1295:1306	arg1	nanocomposites					1308:1321	Ag/CS/silica nanocomposites	1295:1321	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers)	1281:1347	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	8	74	theme	Ag/CS/silica	1295:1306	arg1	nanofibers					1337:1346	Ag/CS/silica nanofibers	1324:1346	Ag/CS/silica nanofibers	1324:1346	The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM).
31881739	2	75	theme	porous	346:351	arg1	scaffolds					366:374	highly porous 3D nanofiber scaffolds	339:374	highly porous 3D nanofiber scaffolds	339:374	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	4	76	theme	materials	694:702	arg1	activity					669:676	The antibacterial activity	651:676	The antibacterial activity of investigated materials	651:702	The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria.
31881739	0	77	theme	Antibacterial	73:85	arg1	Activity					87:94	Antibacterial Activity	73:94	Antibacterial Activity	73:94	Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity.
31881739	7	78	theme	nanocomposite	1252:1264	arg1	type					1244:1247	this type	1239:1247	this type of nanocomposite	1239:1264	The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented.
31881739	2	79	theme	silica	255:260	arg1	phase					262:266	silica phase	255:266	silica phase (CS/silica)	255:278	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	2	79	theme	silica	255:260	arg1	CS/silica					269:277	CS/silica	269:277	CS/silica	269:277	Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds.
31881739	4	80	theme	Gram-positive	721:733	arg1	bacteria					753:760	Gram-positive and Gram-negative bacteria	721:760	Gram-positive and Gram-negative bacteria	721:760	The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria.
30155554	5	0	theme	immune	1086:1091	arg1	cells					1093:1097	the immune cells	1082:1097	the immune cells	1082:1097	The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties.
30155554	3	1	theme	tumor	738:742	arg1	TNF-α					763:767	TNF-α	763:767	TNF-α	763:767	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	1	theme	tumor	738:742	arg1	α					760:760	tumor necrosis factor α	738:760	tumor necrosis factor α (TNF-α)	738:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	2	theme	oxygen	548:553	arg1	species					555:561	reactive oxygen species	539:561	reactive oxygen species	539:561	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	3	theme	species	555:561	arg1	generation					525:534	the generation	521:534	the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase)	521:615	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	4	dep	cytokines	702:710	arg1	[interleukin-1					712:725	[interleukin-1	712:725	[interleukin-1 (IL-1)	712:732	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	4	dep	cytokines	702:710	arg1	TNF-α					763:767	TNF-α	763:767	TNF-α	763:767	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	4	dep	cytokines	702:710	arg1	α					760:760	tumor necrosis factor α	738:760	tumor necrosis factor α (TNF-α)	738:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	4	dep	cytokines	702:710	arg1	IL-1					728:731	IL-1	728:731	IL-1	728:731	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	5	theme	different	442:450	arg1	sources					459:465	different carbon sources	442:465	different carbon sources	442:465	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	1	6	from	animals	193:199	arg1	polysaccharides					114:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	6	from	animals	193:199	arg1	stimulants					144:153	promising stimulants	134:153	promising stimulants of protective functions in humans and animals	134:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	3	7	theme	acid	579:582	arg1	enzymes					570:576	enzymes	570:576	enzymes (acid phosphatase and myeloperoxidase)	570:615	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	7	theme	acid	579:582	arg1	phosphatase					584:594	acid phosphatase	579:594	acid phosphatase	579:594	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	8	theme	carbon	452:457	arg1	sources					459:465	different carbon sources	442:465	different carbon sources	442:465	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	2	9	from	ability	222:228	arg1	1465					302:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	1	10	from	humans	182:187	arg1	polysaccharides					114:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	10	from	humans	182:187	arg1	stimulants					144:153	promising stimulants	134:153	promising stimulants of protective functions in humans and animals	134:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	2	11	theme	resistance	329:338	arg1	factors					340:346	nonspecific resistance factors	317:346	nonspecific resistance factors	317:346	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	5	12	from	effect	1072:1077	arg1	cells					1093:1097	the immune cells	1082:1097	the immune cells	1082:1097	The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties.
30155554	4	13	theme	IL-1β	981:985	arg1	production					957:966	the production	953:966	the production of TNF-α and IL-1β	953:985	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	3	14	theme	necrosis	744:751	arg1	TNF-α					763:767	TNF-α	763:767	TNF-α	763:767	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	14	theme	necrosis	744:751	arg1	α					760:760	tumor necrosis factor α	738:760	tumor necrosis factor α (TNF-α)	738:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	2	15	theme	nonspecific	317:327	arg1	factors					340:346	nonspecific resistance factors	317:346	nonspecific resistance factors	317:346	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	3	16	theme	factor	753:758	arg1	TNF-α					763:767	TNF-α	763:767	TNF-α	763:767	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	16	theme	factor	753:758	arg1	α					760:760	tumor necrosis factor α	738:760	tumor necrosis factor α (TNF-α)	738:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	1	17	from	functions	169:177	arg1	humans					182:187	humans	182:187	humans	182:187	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	17	from	functions	169:177	arg1	animals					193:199	animals	193:199	animals	193:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	18	theme	Bacterial	104:112	arg1	polysaccharides					114:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	18	theme	Bacterial	104:112	arg1	stimulants					144:153	promising stimulants	134:153	promising stimulants of protective functions in humans and animals	134:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	7	19	theme	immunomodulatory	1347:1362	arg1	substance					1364:1372	a biologically active immunomodulatory substance	1325:1372	a biologically active immunomodulatory substance	1325:1372	Therefore, it can find application as a biologically active immunomodulatory substance.
30155554	7	19	theme	immunomodulatory	1347:1362	arg1	application					1310:1320	application	1310:1320	application	1310:1320	Therefore, it can find application as a biologically active immunomodulatory substance.
30155554	0	20	theme	Immunomodulatory	0:15	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.	0:102	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	4	21	theme	metabolic	877:885	arg1	processes					887:895	metabolic processes	877:895	metabolic processes	877:895	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	1	22	from	stimulants	144:153	arg1	humans					182:187	humans	182:187	humans	182:187	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	22	from	stimulants	144:153	arg1	animals					193:199	animals	193:199	animals	193:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	2	23	from	1465	302:305	arg1	exopolysaccharide					233:249	exopolysaccharide	233:249	exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465	233:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	2	23	from	1465	302:305	arg1	ability					222:228	the ability	218:228	the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism	218:367	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	3	24	theme	phagocytic	475:484	arg1	activity					486:493	the phagocytic activity	471:493	the phagocytic activity of murine macrophages	471:515	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	0	25	theme	exopolysaccharide	29:45	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.	0:102	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	6	26	theme	CCM	1247:1249	arg1	1465					1251:1254	P. polymyxa CCM 1465	1235:1254	P. polymyxa CCM 1465	1235:1254	The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity.
30155554	4	27	theme	cells	860:864	arg1	phagocytosis					834:845	the phagocytosis	830:845	the phagocytosis of bacterial cells	830:864	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	6	28	theme	nonspecific	1265:1275	arg1	immunity					1277:1284	nonspecific immunity	1265:1284	nonspecific immunity	1265:1284	The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity.
30155554	3	29	theme	enzymes	570:576	arg1	generation					525:534	the generation	521:534	the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase)	521:615	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	1	30	theme	promising	134:142	arg1	polysaccharides					114:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	30	theme	promising	134:142	arg1	stimulants					144:153	promising stimulants	134:153	promising stimulants of protective functions in humans and animals	134:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	7	31	theme	active	1340:1345	arg1	substance					1364:1372	a biologically active immunomodulatory substance	1325:1372	a biologically active immunomodulatory substance	1325:1372	Therefore, it can find application as a biologically active immunomodulatory substance.
30155554	7	31	theme	active	1340:1345	arg1	application					1310:1320	application	1310:1320	application	1310:1320	Therefore, it can find application as a biologically active immunomodulatory substance.
30155554	4	32	theme	TNF-α	971:975	arg1	production					957:966	the production	953:966	the production of TNF-α and IL-1β	953:985	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	0	33	from	1465	98:101	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.	0:102	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	2	34	theme	polymyxa	289:296	arg1	1465					302:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	3	35	theme	murine	498:503	arg1	macrophages					505:515	murine macrophages	498:515	murine macrophages	498:515	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	2	36	theme	Paenibacillus	275:287	arg1	1465					302:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	3	37	theme	macrophages	505:515	arg1	activity					486:493	the phagocytic activity	471:493	the phagocytic activity of murine macrophages	471:515	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	0	38	theme	Paenibacillus	71:83	arg1	1465					98:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	3	39	dep	effect	395:400	arg1	vitro					385:389	vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)]	385:769	vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)]	385:769	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	2	40	theme	rhizobacterium	260:273	arg1	1465					302:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	5	41	theme	different	1124:1132	arg1	compositions					1134:1145	their different compositions	1118:1145	their different compositions	1118:1145	The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties.
30155554	3	42	dep	enzymes	570:576	arg1	myeloperoxidase					600:614	myeloperoxidase	600:614	myeloperoxidase	600:614	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	42	dep	enzymes	570:576	arg1	enzymes					570:576	enzymes	570:576	enzymes (acid phosphatase and myeloperoxidase)	570:615	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	42	dep	enzymes	570:576	arg1	phosphatase					584:594	acid phosphatase	579:594	acid phosphatase	579:594	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	0	43	theme	rhizobacterium	56:69	arg1	1465					98:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	3	44	theme	human	774:778	arg1	cells					792:796	human mononuclear cells	774:796	human mononuclear cells	774:796	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	4	45	theme	bacterial	850:858	arg1	cells					860:864	bacterial cells	850:864	bacterial cells	850:864	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	3	46	theme	reactive	539:546	arg1	species					555:561	reactive oxygen species	539:561	reactive oxygen species	539:561	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	47	theme	splenocytes	649:659	arg1	proliferation					625:637	the proliferation	621:637	the proliferation of murine splenocytes	621:659	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	2	48	theme	exopolysaccharide	233:249	arg1	ability					222:228	the ability	218:228	the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism	218:367	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	6	49	theme	polymyxa	1238:1245	arg1	1465					1251:1254	P. polymyxa CCM 1465	1235:1254	P. polymyxa CCM 1465	1235:1254	The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity.
30155554	1	50	theme	protective	158:167	arg1	functions					169:177	protective functions	158:177	protective functions in humans and animals	158:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	3	51	theme	mononuclear	780:790	arg1	cells					792:796	human mononuclear cells	774:796	human mononuclear cells	774:796	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	52	theme	proinflammatory	686:700	arg1	cytokines					702:710	proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)	686:768	proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)	686:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	6	53	theme	P.	1235:1236	arg1	1465					1251:1254	P. polymyxa CCM 1465	1235:1254	P. polymyxa CCM 1465	1235:1254	The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity.
30155554	1	54	theme	functions	169:177	arg1	polysaccharides					114:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides	104:128	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	1	54	theme	functions	169:177	arg1	stimulants					144:153	promising stimulants	134:153	promising stimulants of protective functions in humans and animals	134:199	Bacterial polysaccharides are promising stimulants of protective functions in humans and animals.
30155554	0	55	theme	CCM	94:96	arg1	1465					98:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	3	56	theme	cytokines	702:710	arg1	synthesis					673:681	the synthesis	669:681	the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)	669:768	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	3	57	theme	murine	642:647	arg1	splenocytes					649:659	murine splenocytes	642:659	murine splenocytes	642:659	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
30155554	6	58	theme	1465	1251:1254	arg1	exopolysaccharide					1214:1230	the exopolysaccharide	1210:1230	the exopolysaccharide of P. polymyxa CCM 1465	1210:1254	The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity.
30155554	0	59	theme	polymyxa	85:92	arg1	1465					98:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	the rhizobacterium Paenibacillus polymyxa CCM 1465	52:101	Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465.
30155554	2	60	theme	CCM	298:300	arg1	1465					302:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	the rhizobacterium Paenibacillus polymyxa CCM 1465	256:305	We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism.
30155554	4	61	dep	human	900:904	arg1	leukocytes					917:926	leukocytes	917:926	leukocytes	917:926	The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β.
30155554	5	62	with	media	1023:1027	arg1	glucose					1034:1040	glucose	1034:1040	glucose	1034:1040	The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties.
30155554	5	62	with	media	1023:1027	arg1	sucrose					1046:1052	sucrose	1046:1052	sucrose	1046:1052	The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties.
30155554	3	63	theme	exopolysaccharide	409:425	arg1	effect					395:400	the effect	391:400	the effect	391:400	We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells.
29853102	0	0	theme	calcium	100:106	arg1	composites					133:142	calcium phosphate-chitosan AZ91D composites	100:142	calcium phosphate-chitosan AZ91D composites	100:142	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	1	1	theme	-chitosan	337:345	arg1	composite					371:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	4	2	theme	early	894:898	arg1	stage					900:904	the early stage	890:904	the early stage (16 days and before)	890:925	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	5	3	theme	GNS-CaP-CS/AZ91D	1131:1146	arg1	composites					1148:1157	GNS-CaP-CS/AZ91D composites	1131:1157	GNS-CaP-CS/AZ91D composites	1131:1157	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	3	4	theme	electrochemical	511:525	arg1	test					527:530	electrochemical test	511:530	electrochemical test	511:530	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	3	5	theme	immersion	806:814	arg1	process					816:822	the whole immersion process	796:822	the whole immersion process of GNS-CaP-CS/AZ91D	796:842	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	4	6	theme	calcium	992:998	arg1	deposition					1008:1017	calcium mineral deposition	992:1017	calcium mineral deposition	992:1017	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	7	theme	test	527:530	arg1	results					558:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	0	8	theme	AZ91D	127:131	arg1	composites					133:142	calcium phosphate-chitosan AZ91D composites	100:142	calcium phosphate-chitosan AZ91D composites	100:142	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	5	9	theme	PI3K/Akt	1292:1299	arg1	signaling					1301:1309	PI3K/Akt signaling	1292:1309	PI3K/Akt signaling	1292:1309	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	3	10	theme	SEM	554:556	arg1	results					558:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	4	11	theme	stem	1092:1095	arg1	hBMSCs					1104:1109	hBMSCs	1104:1109	hBMSCs	1104:1109	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	11	theme	stem	1092:1095	arg1	cells					1097:1101	mesenchymal stem cells	1080:1101	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	1	12	theme	micro-arc	271:279	arg1	composite					371:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	0	13	theme	phosphate-chitosan	108:125	arg1	composites					133:142	calcium phosphate-chitosan AZ91D composites	100:142	calcium phosphate-chitosan AZ91D composites	100:142	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	1	14	theme	oxidized-AZ91D	281:294	arg1	composite					371:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	4	15	theme	marrow	1073:1078	arg1	deposition					1008:1017	calcium mineral deposition	992:1017	calcium mineral deposition	992:1017	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	15	theme	marrow	1073:1078	arg1	expression					1048:1057	osteoblast-related genes expression	1023:1057	osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs)	1023:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	15	theme	marrow	1073:1078	arg1	activity					982:989	alkaline phosphatase (ALP) activity	955:989	alkaline phosphatase (ALP) activity	955:989	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	16	theme	concentration	536:548	arg1	results					558:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results	507:564	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	1	17	theme	magnesium	210:218	arg1	alloy					225:229	magnesium (Mg) alloy	210:229	magnesium (Mg) alloy	210:229	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	3	18	theme	GNS-CaP-CS/AZ91D	827:842	arg1	process					816:822	the whole immersion process	796:822	the whole immersion process of GNS-CaP-CS/AZ91D	796:842	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	1	19	theme	GNS-CaP-CS/AZ91D	353:368	arg1	composite					371:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	6	20	theme	osteogenic	1385:1394	arg1	differentiation					1396:1410	osteogenic differentiation	1385:1410	osteogenic differentiation	1385:1410	Moreover, Mg element could promote osteogenic differentiation through ERK/MAPK pathway and the maximum concentration was about the range between 200 and 500 ppm.
29853102	5	21	dep	signaling	1301:1309	arg1	stage					1343:1347	the early stage	1333:1347	the early stage	1333:1347	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	5	22	theme	immersion	1266:1274	arg1	process					1276:1282	the long-term immersion process	1252:1282	the long-term immersion process	1252:1282	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	3	23	theme	Good	732:735	arg1	biocompatibility					737:752	16 d. Good biocompatibility	726:752	16 d. Good biocompatibility	726:752	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	6	24	theme	maximum	1445:1451	arg1	concentration					1453:1465	the maximum concentration	1441:1465	the maximum concentration	1441:1465	Moreover, Mg element could promote osteogenic differentiation through ERK/MAPK pathway and the maximum concentration was about the range between 200 and 500 ppm.
29853102	4	25	theme	alkaline	955:962	arg1	ALP					977:979	ALP	977:979	ALP	977:979	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	25	theme	alkaline	955:962	arg1	phosphatase					964:974	alkaline phosphatase	955:974	alkaline phosphatase (ALP) activity	955:989	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	5	26	theme	osteogenic	1197:1206	arg1	differentiation					1208:1222	osteogenic differentiation	1197:1222	osteogenic differentiation	1197:1222	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	4	27	theme	genes	1042:1046	arg1	expression					1048:1057	osteoblast-related genes expression	1023:1057	osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs)	1023:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	28	theme	corrosion	582:590	arg1	rate					592:595	the corrosion rate	578:595	the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity	578:773	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	0	29	theme	biocompatibility	24:39	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet	0:92	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	1	30	theme	MAO-AZ91D	297:305	arg1	composite					371:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	4	31	dep	marrow	1073:1078	arg1	hBMSCs					1104:1109	hBMSCs	1104:1109	hBMSCs	1104:1109	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	31	dep	marrow	1073:1078	arg1	cells					1097:1101	mesenchymal stem cells	1080:1101	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	1	32	theme	alloy	225:229	arg1	osteoinductivity					190:205	osteoinductivity	190:205	osteoinductivity of magnesium (Mg) alloy	190:229	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	32	theme	alloy	225:229	arg1	performance					174:184	the biodegradable performance	156:184	the biodegradable performance	156:184	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	0	33	theme	long-term	14:22	arg1	biocompatibility					24:39	long-term biocompatibility	14:39	long-term biocompatibility	14:39	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	3	34	theme	d.	729:730	arg1	biocompatibility					737:752	16 d. Good biocompatibility	726:752	16 d. Good biocompatibility	726:752	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	2	35	theme	in	413:414	arg1	biocompatibility					422:437	Their long-term in vitro biocompatibility	397:437	Their long-term in vitro biocompatibility	397:437	Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated.
29853102	0	36	theme	osteogenic	45:54	arg1	differentiation					56:70	osteogenic differentiation	45:70	osteogenic differentiation	45:70	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	1	37	theme	based	308:312	arg1	CS					348:349	CS	348:349	CS	348:349	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	37	theme	based	308:312	arg1	-chitosan					337:345	based calcium phosphate (CaP)-chitosan	308:345	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	4	38	theme	phosphatase	964:974	arg1	activity					982:989	alkaline phosphatase (ALP) activity	955:989	alkaline phosphatase (ALP) activity	955:989	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	39	theme	immersion	665:673	arg1	time					675:678	immersion time	665:678	immersion time	665:678	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	3	40	theme	GNS-CaP-CS/AZ91D	600:615	arg1	rate					592:595	the corrosion rate	578:595	the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity	578:773	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	2	41	theme	long-term	403:411	arg1	biocompatibility					422:437	Their long-term in vitro biocompatibility	397:437	Their long-term in vitro biocompatibility	397:437	Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated.
29853102	2	42	theme	signaling	473:481	arg1	pathway					483:489	the related signaling pathway	461:489	the related signaling pathway	461:489	Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated.
29853102	1	43	theme	calcium	314:320	arg1	CS					348:349	CS	348:349	CS	348:349	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	43	theme	calcium	314:320	arg1	-chitosan					337:345	based calcium phosphate (CaP)-chitosan	308:345	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	4	44	dep	stage	900:904	arg1	16 days					907:913	16 days	907:913	16 days and before	907:924	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	45	theme	corrosion	643:651	arg1	degree					653:658	degree	653:658	degree	653:658	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	4	46	theme	bone	1068:1071	arg1	marrow					1073:1078	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	5	47	theme	ERK/MAPK	1232:1239	arg1	pathway					1241:1247	ERK/MAPK pathway	1232:1247	ERK/MAPK pathway	1232:1247	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	1	48	theme	novel	234:238	arg1	GNS					260:262	GNS	260:262	GNS	260:262	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	48	theme	novel	234:238	arg1	nanosheet					249:257	a novel graphene nanosheet	232:257	a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	232:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	2	49	theme	related	465:471	arg1	pathway					483:489	the related signaling pathway	461:489	the related signaling pathway	461:489	Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated.
29853102	1	50	theme	phosphate	322:330	arg1	CS					348:349	CS	348:349	CS	348:349	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	50	theme	phosphate	322:330	arg1	-chitosan					337:345	based calcium phosphate (CaP)-chitosan	308:345	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	6	51	theme	ERK/MAPK	1420:1427	arg1	pathway					1429:1435	ERK/MAPK pathway	1420:1435	ERK/MAPK pathway	1420:1435	Moreover, Mg element could promote osteogenic differentiation through ERK/MAPK pathway and the maximum concentration was about the range between 200 and 500 ppm.
29853102	4	52	from	GNS-CaP-CS/AZ91D	859:874	arg1	stage					900:904	the early stage	890:904	the early stage (16 days and before)	890:925	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	52	from	GNS-CaP-CS/AZ91D	859:874	arg1	Extracts					845:852	Extracts	845:852	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before)	845:925	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	0	53	theme	differentiation	56:70	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet	0:92	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	4	54	dep	16 days	907:913	arg1	before					919:924	before	919:924	before	919:924	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	2	55	dep	in	413:414	arg1	vitro					416:420	vitro	416:420	vitro	416:420	Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated.
29853102	5	56	theme	critical	1173:1180	arg1	role					1182:1185	a critical role	1171:1185	a critical role	1171:1185	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	4	57	theme	osteoblast-related	1023:1040	arg1	expression					1048:1057	osteoblast-related genes expression	1023:1057	osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs)	1023:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	1	58	theme	CaP	333:335	arg1	CS					348:349	CS	348:349	CS	348:349	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	58	theme	CaP	333:335	arg1	-chitosan					337:345	based calcium phosphate (CaP)-chitosan	308:345	micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	271:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	5	59	theme	long-term	1256:1264	arg1	process					1276:1282	the long-term immersion process	1252:1282	the long-term immersion process	1252:1282	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	6	60	theme	Mg	1360:1361	arg1	element					1363:1369	Mg element	1360:1369	Mg element	1360:1369	Moreover, Mg element could promote osteogenic differentiation through ERK/MAPK pathway and the maximum concentration was about the range between 200 and 500 ppm.
29853102	1	61	theme	graphene	240:247	arg1	GNS					260:262	GNS	260:262	GNS	260:262	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	61	theme	graphene	240:247	arg1	nanosheet					249:257	a novel graphene nanosheet	232:257	a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite	232:379	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	1	62	theme	biodegradable	160:172	arg1	performance					174:184	the biodegradable performance	156:184	the biodegradable performance	156:184	To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated.
29853102	0	63	theme	nanosheet	84:92	arg1	biocompatibility					24:39	long-term biocompatibility	14:39	long-term biocompatibility	14:39	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	0	63	theme	nanosheet	84:92	arg1	differentiation					56:70	osteogenic differentiation	45:70	osteogenic differentiation	45:70	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	5	64	attach	released	1117:1124	arg2	GNS					1113:1115	GNS	1113:1115	GNS released from GNS-CaP-CS/AZ91D composites	1113:1157	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	5	64	attach	released	1117:1124	arg1	composites					1148:1157	GNS-CaP-CS/AZ91D composites	1131:1157	GNS-CaP-CS/AZ91D composites	1131:1157	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	5	65	theme	early	1337:1341	arg1	stage					1343:1347	the early stage	1333:1347	the early stage	1333:1347	GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage.
29853102	0	66	theme	graphene	75:82	arg1	nanosheet					84:92	graphene nanosheet	75:92	graphene nanosheet	75:92	Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
29853102	4	67	theme	mineral	1000:1006	arg1	deposition					1008:1017	calcium mineral deposition	992:1017	calcium mineral deposition	992:1017	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	68	theme	human	1062:1066	arg1	marrow					1073:1078	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	69	theme	Mg	533:534	arg1	concentration					536:548	Mg concentration	533:548	Mg concentration	533:548	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
29853102	4	70	theme	mesenchymal	1080:1090	arg1	hBMSCs					1104:1109	hBMSCs	1104:1109	hBMSCs	1104:1109	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	4	70	theme	mesenchymal	1080:1090	arg1	cells					1097:1101	mesenchymal stem cells	1080:1101	human bone marrow mesenchymal stem cells (hBMSCs)	1062:1110	Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs).
29853102	3	71	theme	whole	800:804	arg1	process					816:822	the whole immersion process	796:822	the whole immersion process of GNS-CaP-CS/AZ91D	796:842	The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D.
31284002	0	0	theme	biomedical	112:121	arg1	applications					123:134	biomedical applications	112:134	biomedical applications	112:134	Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
31284002	8	1	dep	composed	1513:1520	arg1	hydrogel					1522:1529	hydrogel	1522:1529	hydrogel	1522:1529	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	8	1	dep	composed	1513:1520	arg1	PVA-Cs-Ag					1503:1511	PVA-Cs-Ag	1503:1511	PVA-Cs-Ag composed hydrogel membranes	1503:1539	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	8	1	dep	composed	1513:1520	arg1	membranes					1531:1539	membranes	1531:1539	membranes	1531:1539	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	2	2	theme	hydrogel	409:416	arg1	membranes					418:426	the prepared composite hydrogel membranes	386:426	the prepared composite hydrogel membranes	386:426	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	10	3	theme	composite	1759:1767	arg1	membranes					1769:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	10	4	theme	satisfactory	1786:1797	arg1	properties					1799:1808	satisfactory properties	1786:1808	satisfactory properties for use as wound dressing materials	1786:1844	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	2	5	theme	composite	399:407	arg1	membranes					418:426	the prepared composite hydrogel membranes	386:426	the prepared composite hydrogel membranes	386:426	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	6	6	dep	%	1185:1185	arg1	20					1183:1184	20	1183:1184	20	1183:1184	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	10	7	theme	dressing	1827:1834	arg1	materials					1836:1844	wound dressing materials	1821:1844	wound dressing materials	1821:1844	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	2	8	theme	blend	330:334	arg1	Chitosan					290:297	Chitosan	290:297	Chitosan	290:297	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	2	8	theme	blend	330:334	arg1	materials					336:344	antimicrobial blend materials	316:344	antimicrobial blend materials	316:344	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	4	9	theme	hydrogel	668:675	arg1	membranes					677:685	hydrogel membranes	668:685	hydrogel membranes	668:685	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	4	10	theme	membranes	813:821	arg1	concentration					716:728	copolymer concentration	706:728	copolymer concentration	706:728	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	4	10	theme	membranes	813:821	arg1	dose					743:746	irradiation dose	731:746	irradiation dose	731:746	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	4	10	theme	membranes	813:821	arg1	concentration					755:767	AgNO3 concentration	749:767	AgNO3 concentration	749:767	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	4	10	theme	membranes	813:821	arg1	E					795:795	vitamin E	787:795	vitamin E	787:795	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	4	10	theme	membranes	813:821	arg1	plasticizer					770:780	plasticizer	770:780	plasticizer	770:780	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	2	11	theme	antimicrobial	316:328	arg1	Chitosan					290:297	Chitosan	290:297	Chitosan	290:297	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	2	11	theme	antimicrobial	316:328	arg1	materials					336:344	antimicrobial blend materials	316:344	antimicrobial blend materials	316:344	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	10	12	theme	wound	1821:1825	arg1	materials					1836:1844	wound dressing materials	1821:1844	wound dressing materials	1821:1844	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	7	13	theme	membranes	1340:1348	arg1	ratio					1313:1317	The swelling ratio	1300:1317	The swelling ratio of composed hydrogel membranes	1300:1348	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	8	14	from	activity	1574:1581	arg1	particular					1586:1595	particular	1586:1595	particular	1586:1595	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	2	15	theme	prepared	390:397	arg1	membranes					418:426	the prepared composite hydrogel membranes	386:426	the prepared composite hydrogel membranes	386:426	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	7	16	theme	radiation	1388:1396	arg1	dose					1398:1401	radiation dose	1388:1401	radiation dose	1388:1401	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	3	17	theme	composite	589:597	arg1	membranes					599:607	hydrogel composite membranes	580:607	hydrogel composite membranes	580:607	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	4	18	theme	AgNO3	749:753	arg1	concentration					755:767	AgNO3 concentration	749:767	AgNO3 concentration	749:767	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	5	19	theme	membranes	937:945	arg1	properties					904:913	the mechanical and thermal properties	877:913	the mechanical and thermal properties of hydrogel composite membranes	877:945	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	3	20	theme	membranes	599:607	arg1	morphology					566:575	the morphology	562:575	the morphology of hydrogel composite membranes	562:607	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	4	21	theme	irradiation	731:741	arg1	dose					743:746	irradiation dose	731:746	irradiation dose	731:746	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	0	22	theme	radiation-induced	6:22	arg1	Gamma					0:4	Gamma radiation-induced	0:22	Gamma radiation-induced	0:22	Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
31284002	6	23	theme	AgNP	1215:1218	arg1	incorporation					1220:1232	AgNP incorporation	1215:1232	AgNP incorporation due to the viscosity of copolymer composition	1215:1278	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	2	24	theme	further	357:363	arg1	biocompatibility					365:380	further biocompatibility	357:380	further biocompatibility for the prepared composite hydrogel membranes	357:426	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	1	25	theme	chitosan-loaded	215:229	arg1	AgNO3					231:235	chitosan-loaded AgNO3	215:235	chitosan-loaded AgNO3	215:235	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	5	26	theme	mechanical	881:890	arg1	properties					904:913	the mechanical and thermal properties	877:913	the mechanical and thermal properties of hydrogel composite membranes	877:945	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	7	27	theme	swelling	1304:1311	arg1	ratio					1313:1317	The swelling ratio	1300:1317	The swelling ratio of composed hydrogel membranes	1300:1348	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	4	28	theme	PVA/chitosan	800:811	arg1	membranes					813:821	PVA/chitosan membranes	800:821	PVA/chitosan membranes	800:821	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	3	29	theme	gamma	489:493	arg1	irradiation					495:505	gamma irradiation	489:505	gamma irradiation	489:505	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	0	30	theme	composite	36:44	arg1	membranes					46:54	composite membranes	36:54	composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications	36:134	Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
31284002	7	31	theme	hydrogel	1483:1490	arg1	membranes					1492:1500	formed hydrogel membranes	1476:1500	formed hydrogel membranes	1476:1500	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	10	32	theme	E	1748:1748	arg1	membranes					1769:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	1	33	theme	Crosslinked	137:147	arg1	membranes					168:176	Crosslinked hydrogel composite membranes	137:176	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E	137:249	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	4	34	theme	copolymer	706:714	arg1	concentration					716:728	copolymer concentration	706:728	copolymer concentration	706:728	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	3	35	theme	crosslinking	433:444	arg1	reaction					446:453	The crosslinking reaction	429:453	The crosslinking reaction between PVA and chitosan owing to gamma irradiation	429:505	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	4	36	theme	Important	634:642	arg1	parameters					644:653	Important parameters	634:653	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes	634:821	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	7	37	theme	membranes	1492:1500	arg1	degree					1466:1471	the crosslinking degree	1449:1471	the crosslinking degree of formed hydrogel membranes	1449:1500	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	1	38	theme	hydrogel	149:156	arg1	membranes					168:176	Crosslinked hydrogel composite membranes	137:176	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E	137:249	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	8	39	attach	presence	1637:1644	arg1	membranes					1657:1665	membranes	1657:1665	membranes	1657:1665	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	8	39	attach	presence	1637:1644	arg2	AgNP					1649:1652	AgNP	1649:1652	AgNP	1649:1652	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	1	40	theme	vitamin	241:247	arg1	E					249:249	vitamin E	241:249	vitamin E	241:249	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	8	41	theme	other	1680:1684	arg1	microbes					1705:1712	other bacteria and fungi microbes	1680:1712	other bacteria and fungi microbes	1680:1712	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	6	42	theme	PVA	1122:1124	arg1	composition					1126:1136	PVA composition	1122:1136	PVA composition	1122:1136	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	7	43	dep	dose	1398:1401	arg1	the					1384:1386	the	1384:1386	the	1384:1386	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	6	44	theme	irradiation	1139:1149	arg1	dose					1151:1154	irradiation dose	1139:1154	irradiation dose	1139:1154	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	5	45	theme	hydrogel	918:925	arg1	membranes					937:945	hydrogel composite membranes	918:945	hydrogel composite membranes	918:945	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	6	46	theme	glycerol	1160:1167	arg1	content					1169:1175	glycerol content	1160:1175	glycerol content	1160:1175	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	8	47	from	presence	1637:1644	arg1	membranes					1657:1665	membranes	1657:1665	membranes	1657:1665	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	8	48	theme	significant	1548:1558	arg1	activity					1574:1581	significant antimicrobial activity	1548:1581	significant antimicrobial activity in particular against Streptococcus mutans	1548:1624	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	6	49	theme	membranes	1084:1092	arg1	%					1069:1069	%	1069:1069	%	1069:1069	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	6	49	theme	membranes	1084:1092	arg1	gelation					1059:1066	the gelation	1055:1066	the gelation (%) of hydrogel membranes	1055:1092	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	5	50	theme	composite	927:935	arg1	membranes					937:945	hydrogel composite membranes	918:945	hydrogel composite membranes	918:945	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	0	51	theme	polyvinyl	65:73	arg1	E					106:106	polyvinyl alcohol/chitosan/AgNO3/vitamin E	65:106	polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications	65:134	Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
31284002	8	52	theme	AgNP	1649:1652	arg1	presence					1637:1644	the presence	1633:1644	the presence of AgNP in membranes	1633:1665	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	6	53	dep	20	1183:1184	arg1	to					1180:1181	to	1180:1181	to	1180:1181	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	1	54	theme	composite	158:166	arg1	membranes					168:176	Crosslinked hydrogel composite membranes	137:176	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E	137:249	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	7	55	theme	formed	1476:1481	arg1	membranes					1492:1500	formed hydrogel membranes	1476:1500	formed hydrogel membranes	1476:1500	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	8	56	theme	bacteria	1686:1693	arg1	microbes					1705:1712	other bacteria and fungi microbes	1680:1712	other bacteria and fungi microbes	1680:1712	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	8	57	theme	antimicrobial	1560:1572	arg1	activity					1574:1581	significant antimicrobial activity	1548:1581	significant antimicrobial activity in particular against Streptococcus mutans	1548:1624	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	5	58	theme	application	996:1006	arg1	possibility					977:987	the possibility	973:987	the possibility of its application for wound dressings	973:1026	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	7	59	theme	composed	1322:1329	arg1	membranes					1340:1348	composed hydrogel membranes	1322:1348	composed hydrogel membranes	1322:1348	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	4	60	theme	vitamin	787:793	arg1	E					795:795	vitamin E	787:795	vitamin E	787:795	Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, AgNO3 concentration, plasticizer, and vitamin E of PVA/chitosan membranes were evaluated and discussed in details.
31284002	7	61	theme	due	1430:1432	arg1	AgNP					1424:1427	AgNP	1424:1427	AgNP	1424:1427	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	6	62	theme	composition	1268:1278	arg1	viscosity					1245:1253	the viscosity	1241:1253	the viscosity of copolymer composition	1241:1278	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	7	63	theme	crosslinking	1453:1464	arg1	degree					1466:1471	the crosslinking degree	1449:1471	the crosslinking degree of formed hydrogel membranes	1449:1500	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	3	64	theme	FTIR	541:544	arg1	analysis					546:553	FTIR analysis	541:553	FTIR analysis	541:553	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	7	65	theme	degree	1466:1471	arg1	reducing					1437:1444	reducing	1437:1444	reducing of the crosslinking degree of formed hydrogel membranes	1437:1500	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	8	66	theme	fungi	1699:1703	arg1	microbes					1705:1712	other bacteria and fungi microbes	1680:1712	other bacteria and fungi microbes	1680:1712	PVA-Cs-Ag composed hydrogel membranes showed significant antimicrobial activity in particular against Streptococcus mutans due to the presence of AgNP in membranes, compared to other bacteria and fungi microbes.
31284002	6	67	dep	composition	1126:1136	arg1	%					1185:1185	%	1185:1185	%	1185:1185	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	6	68	theme	copolymer	1258:1266	arg1	composition					1268:1278	copolymer composition	1258:1278	copolymer composition	1258:1278	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
31284002	5	69	theme	wound	1012:1016	arg1	dressings					1018:1026	wound dressings	1012:1026	wound dressings	1012:1026	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	7	70	theme	hydrogel	1331:1338	arg1	membranes					1340:1348	composed hydrogel membranes	1322:1348	composed hydrogel membranes	1322:1348	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	1	71	link	Crosslinked	137:147	arg1	membranes					168:176	Crosslinked hydrogel composite membranes	137:176	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E	137:249	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	7	72	theme	AgNP	1424:1427	arg1	incorporation					1407:1419	incorporation	1407:1419	incorporation	1407:1419	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	7	72	theme	AgNP	1424:1427	arg1	dose					1398:1401	radiation dose	1388:1401	radiation dose	1388:1401	The swelling ratio of composed hydrogel membranes decreased notably with increasing the radiation dose and incorporation of AgNP, due to reducing of the crosslinking degree of formed hydrogel membranes.
31284002	1	73	theme	polyvinyl	187:195	arg1	alcohol					197:203	polyvinyl alcohol	187:203	polyvinyl alcohol (PVA)	187:209	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	1	73	theme	polyvinyl	187:195	arg1	PVA					206:208	PVA	206:208	PVA	206:208	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	10	74	theme	hydrogel	1750:1757	arg1	membranes					1769:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes	1748:1777	E hydrogel composite membranes showed satisfactory properties for use as wound dressing materials.
31284002	1	75	theme	gamma	271:275	arg1	irradiation					277:287	gamma irradiation	271:287	gamma irradiation	271:287	Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation.
31284002	0	76	theme	alcohol/chitosan/AgNO3/vitamin	75:104	arg1	E					106:106	polyvinyl alcohol/chitosan/AgNO3/vitamin E	65:106	polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications	65:134	Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
31284002	5	77	theme	thermal	896:902	arg1	properties					904:913	the mechanical and thermal properties	877:913	the mechanical and thermal properties of hydrogel composite membranes	877:945	In addition, the mechanical and thermal properties of hydrogel composite membranes were examined to evaluate the possibility of its application for wound dressings.
31284002	3	78	theme	hydrogel	580:587	arg1	membranes					599:607	hydrogel composite membranes	580:607	hydrogel composite membranes	580:607	The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM.
31284002	2	79	used	used	308:311	arg2	Chitosan					290:297	Chitosan	290:297	Chitosan	290:297	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	2	79	used	used	308:311	arg2	materials					336:344	antimicrobial blend materials	316:344	antimicrobial blend materials	316:344	Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes.
31284002	6	80	theme	hydrogel	1075:1082	arg1	membranes					1084:1092	hydrogel membranes	1075:1092	hydrogel membranes	1075:1092	The results revealed that the gelation (%) of hydrogel membranes increased dramatically with PVA composition, irradiation dose and glycerol content up to 20%; however, it decreased with AgNP incorporation due to the viscosity of copolymer composition is hyper-increased.
29542910	9	0	theme	gels	1635:1638	arg1	cytocompatibility					1610:1626	The cytocompatibility	1606:1626	The cytocompatibility of the gels	1606:1638	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	1	1	theme	physiological	257:269	arg1	pH					271:272	physiological pH	257:272	physiological pH	257:272	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	6	2	theme	final	1286:1290	arg1	gel					1292:1294	the final gel	1282:1294	the final gel	1282:1294	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	3	theme	amorphous/crystalline	841:861	arg1	morphology					863:872	a moderately amorphous/crystalline morphology	828:872	a moderately amorphous/crystalline morphology	828:872	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	9	4	theme	cell	1672:1675	arg1	WI-38					1689:1693	WI-38	1689:1693	WI-38	1689:1693	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	9	4	theme	cell	1672:1675	arg1	line					1677:1680	the fibroblast cell line	1657:1680	the fibroblast cell line (i.e., WI-38)	1657:1694	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	7	5	theme	elastic	1401:1407	arg1	behavior					1409:1416	the enhanced elastic behavior	1388:1416	the enhanced elastic behavior	1388:1416	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	5	6	theme	acid	886:889	arg1	grafting					891:898	amino acid grafting	880:898	amino acid grafting	880:898	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	5	7	theme	scanning	726:733	arg1	calorimetry-					735:746	Differential scanning calorimetry-	713:746	Differential scanning calorimetry-	713:746	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	8	8	theme	month	1470:1474	arg1	period					1487:1492	a 1 month incubation period	1466:1492	a 1 month incubation period	1466:1492	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	6	9	theme	total	1259:1263	arg1	content					1271:1277	total solid content	1259:1277	total solid content in the final gel	1259:1294	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	2	10	theme	carbodiimide	493:504	arg1	reaction					515:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	9	11	theme	good	1710:1713	arg1	viability					1720:1728	good cell viability	1710:1728	good cell viability	1710:1728	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	9	12	dep	WI-38	1689:1693	arg1	i.e.					1683:1686	i.e.	1683:1686	i.e.	1683:1686	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	7	13	theme	total	1360:1364	arg1	content					1372:1378	total solid content	1360:1378	the lowest cross-linker as well as total solid content	1325:1378	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	0	14	theme	Fast	60:63	arg1	Gelation					65:72	in Situ Fast Gelation	52:72	in Situ Fast Gelation	52:72	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	4	15	theme	reagent	697:703	arg1	ratios					705:710	the feeding reagent ratios	685:710	the feeding reagent ratios	685:710	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	5	16	theme	pH.	958:960	arg1	hydrogels					972:980	physiological pH. Composite hydrogels	944:980	physiological pH. Composite hydrogels gelated within 60 s	944:1000	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	8	17	theme	remaining	1535:1543	arg1	weight					1528:1533	weight remaining	1528:1543	weight remaining of 60 ± 1.5	1528:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	0	18	theme	Future	102:107	arg1	Biomaterial					120:130	Future Injectable Biomaterial	102:130	Future Injectable Biomaterial	102:130	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	7	19	from	content	1372:1378	arg1	injectable					1311:1320	injectable	1311:1320	injectable	1311:1320	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	7	19	from	content	1372:1378	arg1	gels					1301:1304	The gels	1297:1304	The gels	1297:1304	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	2	20	theme	coupling	506:513	arg1	reaction					515:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	0	21	theme	Composite	0:8	arg1	Hydrogels					10:18	Composite Hydrogels	0:18	Composite Hydrogels	0:18	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	8	22	theme	phosphate-buffered	1497:1514	arg1	saline					1516:1521	phosphate-buffered saline	1497:1521	phosphate-buffered saline with weight remaining of 60 ± 1.5	1497:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	2	23	theme	amino	411:415	arg1	acid					417:420	a small anionic amino acid	395:420	a small anionic amino acid	395:420	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	2	23	theme	amino	411:415	arg1	acid					434:437	l-glutamic acid	423:437	l-glutamic acid	423:437	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	7	24	theme	lowest	1329:1334	arg1	cross-linker					1336:1347	the lowest cross-linker	1325:1347	the lowest cross-linker as well as total solid content	1325:1378	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	2	25	theme	single-step	448:458	arg1	reaction					515:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	2	26	theme	small	397:401	arg1	acid					417:420	a small anionic amino acid	395:420	a small anionic amino acid	395:420	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	2	26	theme	small	397:401	arg1	acid					434:437	l-glutamic acid	423:437	l-glutamic acid	423:437	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	0	27	dep	in	52:53	arg1	Situ					55:58	Situ	55:58	Situ	55:58	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	10	28	theme	injectable	1799:1808	arg1	hydrogels					1767:1775	these hydrogels	1761:1775	these hydrogels	1761:1775	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	10	28	theme	injectable	1799:1808	arg1	biomaterial					1810:1820	an important injectable biomaterial	1786:1820	an important injectable biomaterial for delivery purpose in the future	1786:1855	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	0	29	theme	in	52:53	arg1	Gelation					65:72	in Situ Fast Gelation	52:72	in Situ Fast Gelation	52:72	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	6	30	theme	±	1182:1182	arg1	kPa					1201:1203	4.0 ± 1.0 and 31 ± 2.5 kPa	1178:1203	4.0 ± 1.0 and 31 ± 2.5 kPa	1178:1203	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	31	theme	4-arm	1060:1064	arg1	poly					1066:1069	benzaldehyde-terminated 4-arm poly	1036:1069	benzaldehyde-terminated 4-arm poly(ethylene glycol)	1036:1086	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	5	31	theme	4-arm	1060:1064	arg1	glycol					1080:1085	ethylene glycol	1071:1085	ethylene glycol	1071:1085	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	7	32	from	cross-linker	1336:1347	arg1	injectable					1311:1320	injectable	1311:1320	injectable	1311:1320	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	7	32	from	cross-linker	1336:1347	arg1	gels					1301:1304	The gels	1297:1304	The gels	1297:1304	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	4	33	theme	substitution	615:626	arg1	range					628:632	substitution range	615:632	substitution range	615:632	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	8	34	with	saline	1516:1521	arg1	weight					1528:1533	weight remaining	1528:1543	weight remaining of 60 ± 1.5	1528:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	8	35	from	%	1570:1570	arg1	saline					1516:1521	phosphate-buffered saline	1497:1521	phosphate-buffered saline with weight remaining of 60 ± 1.5	1497:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	0	36	with	Approach	38:45	arg1	Gelation					65:72	in Situ Fast Gelation	52:72	in Situ Fast Gelation	52:72	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	0	36	with	Approach	38:45	arg1	Ability					91:97	Self-Healing Ability	78:97	Self-Healing Ability	78:97	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	1	37	theme	low	217:219	arg1	solubility					227:236	the low water solubility	213:236	the low water solubility of most of them at physiological pH	213:272	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	3	38	theme	zwitterion-tethered	544:562	arg1	structure					564:572	a zwitterion-tethered structure	542:572	a zwitterion-tethered structure onto the polymer backbone	542:598	This resulted in a zwitterion-tethered structure onto the polymer backbone.
29542910	2	39	theme	water-soluble	320:332	arg1	derivative					334:343	a water-soluble derivative	318:343	a water-soluble derivative of chitosan (WSC) biopolymer	318:372	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	1	40	theme	biocompatible	184:196	arg1	hydrogels					198:206	biocompatible hydrogels	184:206	biocompatible hydrogels	184:206	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	9	41	theme	fibroblast	1661:1670	arg1	WI-38					1689:1693	WI-38	1689:1693	WI-38	1689:1693	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	9	41	theme	fibroblast	1661:1670	arg1	line					1677:1680	the fibroblast cell line	1657:1680	the fibroblast cell line (i.e., WI-38)	1657:1694	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	8	42	from	pHs	1575:1577	arg1	%					1570:1570	44 ± 1.45%	1561:1570	44 ± 1.45% at pHs 7.4 and 6.5	1561:1589	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	8	42	from	pHs	1575:1577	arg1	biodegradability					1442:1457	biodegradability	1442:1457	biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5	1442:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	3	43	theme	polymer	583:589	arg1	backbone					591:598	the polymer backbone	579:598	the polymer backbone	579:598	This resulted in a zwitterion-tethered structure onto the polymer backbone.
29542910	1	44	from	pH	271:272	arg1	solubility					227:236	the low water solubility	213:236	the low water solubility of most of them at physiological pH	213:272	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	8	45	from	biodegradability	1442:1457	arg1	saline					1516:1521	phosphate-buffered saline	1497:1521	phosphate-buffered saline with weight remaining of 60 ± 1.5	1497:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	5	46	theme	amino	880:884	arg1	grafting					891:898	amino acid grafting	880:898	amino acid grafting	880:898	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	8	47	theme	1	1468:1468	arg1	month					1470:1474	month	1470:1474	month	1470:1474	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	6	48	dep	kPa	1201:1203	arg1	either					1206:1211	either	1206:1211	either	1206:1211	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	49	theme	Differential	713:724	arg1	calorimetry-					735:746	Differential scanning calorimetry-	713:746	Differential scanning calorimetry-	713:746	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	1	50	theme	water	221:225	arg1	solubility					227:236	the low water solubility	213:236	the low water solubility of most of them at physiological pH	213:272	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	8	51	theme	incubation	1476:1485	arg1	period					1487:1492	a 1 month incubation period	1466:1492	a 1 month incubation period	1466:1492	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	6	52	theme	solid	1265:1269	arg1	content					1271:1277	total solid content	1259:1277	total solid content in the final gel	1259:1294	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	53	theme	Composite	962:970	arg1	hydrogels					972:980	physiological pH. Composite hydrogels	944:980	physiological pH. Composite hydrogels gelated within 60 s	944:1000	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	10	54	theme	important	1789:1797	arg1	hydrogels					1767:1775	these hydrogels	1761:1775	these hydrogels	1761:1775	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	10	54	theme	important	1789:1797	arg1	biomaterial					1810:1820	an important injectable biomaterial	1786:1820	an important injectable biomaterial for delivery purpose in the future	1786:1855	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	6	55	theme	cross-linker	1229:1240	arg1	concentration					1242:1254	the cross-linker concentration	1225:1254	the cross-linker concentration	1225:1254	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	6	56	from	content	1271:1277	arg1	gel					1292:1294	the final gel	1282:1294	the final gel	1282:1294	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	1	57	theme	attractive	149:158	arg1	Biopolymers					133:143	Biopolymers	133:143	Biopolymers	133:143	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	1	57	theme	attractive	149:158	arg1	candidates					160:169	attractive candidates	149:169	attractive candidates	149:169	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	9	58	theme	cell	1715:1718	arg1	viability					1720:1728	good cell viability	1710:1728	good cell viability	1710:1728	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	7	59	theme	solid	1366:1370	arg1	content					1372:1378	total solid content	1360:1378	the lowest cross-linker as well as total solid content	1325:1378	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	0	60	theme	Self-Healing	78:89	arg1	Ability					91:97	Self-Healing Ability	78:97	Self-Healing Ability	78:97	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	5	61	theme	physiological	944:956	arg1	hydrogels					972:980	physiological pH. Composite hydrogels	944:980	physiological pH. Composite hydrogels gelated within 60 s	944:1000	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	2	62	theme	1-ethyl-3-[3-dimethylaminopropyl	460:491	arg1	reaction					515:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction	446:522	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	4	63	theme	feeding	689:695	arg1	ratios					705:710	the feeding reagent ratios	685:710	the feeding reagent ratios	685:710	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	6	64	from	concentration	1242:1254	arg1	gel					1292:1294	the final gel	1282:1294	the final gel	1282:1294	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	65	from	amorphous	813:821	arg1	transition					797:806	a transition	795:806	a transition from amorphous into a moderately amorphous/crystalline morphology	795:872	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	9	66	theme	gel	1737:1739	arg1	surface					1741:1747	the gel surface	1733:1747	the gel surface	1733:1747	The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface.
29542910	8	67	theme	±	1551:1551	arg1	weight					1528:1533	weight remaining	1528:1543	weight remaining of 60 ± 1.5	1528:1555	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	0	68	theme	Injectable	109:118	arg1	Biomaterial					120:130	Future Injectable Biomaterial	102:130	Future Injectable Biomaterial	102:130	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	4	69	theme	±	644:644	arg1	%					650:650	13-16 ± 1.25%	638:650	13-16 ± 1.25%	638:650	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	4	69	theme	±	644:644	arg1	degree					605:610	The degree	601:610	The degree of substitution range	601:632	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	6	70	theme	hydrogels	1138:1146	arg1	modulus					1121:1127	The compressive modulus	1105:1127	The compressive modulus of these hydrogels	1105:1146	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	5	71	theme	X-ray	752:756	arg1	analysis					776:783	X-ray diffraction-based analysis	752:783	X-ray diffraction-based analysis	752:783	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	2	72	theme	l-glutamic	423:432	arg1	acid					417:420	a small anionic amino acid	395:420	a small anionic amino acid	395:420	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	2	72	theme	l-glutamic	423:432	arg1	acid					434:437	l-glutamic acid	423:437	l-glutamic acid	423:437	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	8	73	theme	±	1564:1564	arg1	%					1570:1570	44 ± 1.45%	1561:1570	44 ± 1.45% at pHs 7.4 and 6.5	1561:1589	These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively.
29542910	0	74	theme	Bioinspired	26:36	arg1	Approach					38:45	Bioinspired Approach	26:45	Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability	26:97	Composite Hydrogels Using Bioinspired Approach with in Situ Fast Gelation and Self-Healing Ability as Future Injectable Biomaterial.
29542910	6	75	theme	compressive	1109:1119	arg1	modulus					1121:1127	The compressive modulus	1105:1127	The compressive modulus of these hydrogels	1105:1146	The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel.
29542910	10	76	theme	delivery	1826:1833	arg1	purpose					1835:1841	delivery purpose	1826:1841	delivery purpose	1826:1841	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	2	77	theme	anionic	403:409	arg1	acid					417:420	a small anionic amino acid	395:420	a small anionic amino acid	395:420	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	2	77	theme	anionic	403:409	arg1	acid					434:437	l-glutamic acid	423:437	l-glutamic acid	423:437	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	5	78	theme	ethylene	1071:1078	arg1	poly					1066:1069	benzaldehyde-terminated 4-arm poly	1036:1069	benzaldehyde-terminated 4-arm poly(ethylene glycol)	1036:1086	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	5	78	theme	ethylene	1071:1078	arg1	glycol					1080:1085	ethylene glycol	1071:1085	ethylene glycol	1071:1085	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	5	79	theme	diffraction-based	758:774	arg1	analysis					776:783	X-ray diffraction-based analysis	752:783	X-ray diffraction-based analysis	752:783	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	2	80	theme	chitosan	348:355	arg1	biopolymer					363:372	chitosan (WSC) biopolymer	348:372	chitosan (WSC) biopolymer	348:372	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
29542910	5	81	theme	benzaldehyde-terminated	1036:1058	arg1	poly					1066:1069	benzaldehyde-terminated 4-arm poly	1036:1069	benzaldehyde-terminated 4-arm poly(ethylene glycol)	1036:1086	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	5	81	theme	benzaldehyde-terminated	1036:1058	arg1	glycol					1080:1085	ethylene glycol	1071:1085	ethylene glycol	1071:1085	Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker.
29542910	7	82	theme	enhanced	1392:1399	arg1	behavior					1409:1416	the enhanced elastic behavior	1388:1416	the enhanced elastic behavior	1388:1416	The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior.
29542910	4	83	theme	range	628:632	arg1	degree					605:610	The degree	601:610	The degree of substitution range	601:632	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	4	83	theme	range	628:632	arg1	%					650:650	13-16 ± 1.25%	638:650	13-16 ± 1.25%	638:650	The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios.
29542910	1	84	theme	most	241:244	arg1	solubility					227:236	the low water solubility	213:236	the low water solubility of most of them at physiological pH	213:272	Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications.
29542910	10	85	from	biomaterial	1810:1820	arg1	future					1850:1855	future	1850:1855	future	1850:1855	Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future.
29542910	2	86	theme	biopolymer	363:372	arg1	derivative					334:343	a water-soluble derivative	318:343	a water-soluble derivative of chitosan (WSC) biopolymer	318:372	To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction.
30231646	0	0	theme	2-N,6-O-sulphated	90:106	arg1	chitosan					108:115	2-N,6-O-sulphated chitosan	90:115	2-N,6-O-sulphated chitosan	90:115	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	2	1	dep	rhBMP-2	469:475	arg1	the					454:456	the	454:456	the	454:456	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	1	2	theme	bone	174:177	arg1	generation					179:188	bone generation	174:188	bone generation	174:188	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	8	3	theme	angiogenic	1408:1417	arg1	expression					1424:1433	angiogenic gene expression	1408:1433	angiogenic gene expression	1408:1433	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	0	4	from	effects	12:18	arg1	hydrogels					66:74	composite hydrogels	56:74	composite hydrogels	56:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	5	5	dep	showed	814:819	arg1	whereas					867:873	whereas	867:873	whereas	867:873	RhBMP-2 loaded in PLGA microspheres showed a sustained release over a period of 2 weeks, whereas rhVEGF165 loaded in hydrogel eluted almost completely from the hydrogel over the first 16 days.
30231646	2	6	theme	poly	362:365	arg1	microspheres					396:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	6	7	theme	growth	976:981	arg1	factors					983:989	Both growth factors	971:989	Both growth factors	971:989	Both growth factors retained their efficacy, as quantified with relevant in vitro assays.
30231646	10	8	from	worthy	1696:1701	arg1	research					1734:1741	subsequent research	1723:1741	subsequent research	1723:1741	From our findings, we could also conclude that rhBMP-2 can promote angiogenesis and that the mechanism is worthy of further study in subsequent research.
30231646	7	9	theme	cell	1083:1086	arg1	response					1088:1095	an enhanced cell response	1071:1095	an enhanced cell response	1071:1095	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	8	10	dep	26SCS	1232:1236	arg1	presence					1220:1227	presence	1220:1227	presence	1220:1227	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	8	10	dep	26SCS	1232:1236	arg1	the					1216:1218	the	1216:1218	the	1216:1218	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	8	11	theme	cell	1349:1352	arg1	proliferation					1354:1366	human umbilical vein endothelial cell proliferation	1316:1366	human umbilical vein endothelial cell proliferation	1316:1366	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	2	12	from	rhBMP-2	469:475	arg1	manner					518:523	the required manner	505:523	the required manner	505:523	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	4	13	theme	factors	739:745	arg1	behaviour					711:719	a similar release behaviour	693:719	a similar release behaviour of the two growth factors	693:745	The system showed a similar release behaviour of the two growth factors regardless of 26SCS inclusion.
30231646	1	14	theme	controlled	274:283	arg1	mode					315:318	a controlled spatial and temporal delivery mode	272:318	a controlled spatial and temporal delivery mode	272:318	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	4	15	theme	26SCS	761:765	arg1	inclusion					767:775	26SCS inclusion	761:775	26SCS inclusion	761:775	The system showed a similar release behaviour of the two growth factors regardless of 26SCS inclusion.
30231646	8	16	theme	phosphatase	1294:1304	arg1	activity					1306:1313	significantly upregulated alkaline phosphatase activity	1259:1313	significantly upregulated alkaline phosphatase activity	1259:1313	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	0	17	theme	bone	120:123	arg1	regeneration					125:136	bone regeneration	120:136	bone regeneration	120:136	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	2	18	theme	angiogenic	481:490	arg1	rhVEGF165					492:500	angiogenic rhVEGF165	481:500	angiogenic rhVEGF165	481:500	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	3	19	theme	2-N,6-O-sulphated	539:555	arg1	chitosan					557:564	2-N,6-O-sulphated chitosan	539:564	2-N,6-O-sulphated chitosan (26SCS)	539:572	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	3	19	theme	2-N,6-O-sulphated	539:555	arg1	factor					597:602	a synergetic factor	584:602	a synergetic factor of growth factors	584:620	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	3	19	theme	2-N,6-O-sulphated	539:555	arg1	26SCS					567:571	26SCS	567:571	26SCS	567:571	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	3	19	theme	2-N,6-O-sulphated	539:555	arg1	well					669:672	well	669:672	well	669:672	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	7	20	theme	factors	1143:1149	arg1	delivery					1119:1126	the delivery	1115:1126	the delivery of dual growth factors	1115:1149	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	10	21	theme	subsequent	1723:1732	arg1	research					1734:1741	subsequent research	1723:1741	subsequent research	1723:1741	From our findings, we could also conclude that rhBMP-2 can promote angiogenesis and that the mechanism is worthy of further study in subsequent research.
30231646	10	22	theme	study	1714:1718	arg1	mechanism					1683:1691	the mechanism	1679:1691	the mechanism	1679:1691	From our findings, we could also conclude that rhBMP-2 can promote angiogenesis and that the mechanism is worthy of further study in subsequent research.
30231646	10	22	theme	study	1714:1718	arg1	worthy					1696:1701	worthy	1696:1701	worthy of further study in subsequent research	1696:1741	From our findings, we could also conclude that rhBMP-2 can promote angiogenesis and that the mechanism is worthy of further study in subsequent research.
30231646	7	23	theme	single	1185:1190	arg1	factor					1192:1197	a single factor	1183:1197	a single factor	1183:1197	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	8	24	theme	endothelial	1337:1347	arg1	proliferation					1354:1366	human umbilical vein endothelial cell proliferation	1316:1366	human umbilical vein endothelial cell proliferation	1316:1366	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	2	25	theme	PLGA	390:393	arg1	microspheres					396:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	0	26	theme	Synergistic	0:10	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of dual growth factor delivery from composite hydrogels	0:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	1	27	theme	spatial	285:291	arg1	mode					315:318	a controlled spatial and temporal delivery mode	272:318	a controlled spatial and temporal delivery mode	272:318	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	6	28	dep	in	1044:1045	arg1	vitro					1047:1051	vitro	1047:1051	vitro	1047:1051	Both growth factors retained their efficacy, as quantified with relevant in vitro assays.
30231646	8	29	theme	umbilical	1322:1330	arg1	proliferation					1354:1366	human umbilical vein endothelial cell proliferation	1316:1366	human umbilical vein endothelial cell proliferation	1316:1366	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	5	30	theme	2 weeks	858:864	arg1	period					848:853	a period	846:853	a period of 2 weeks	846:864	RhBMP-2 loaded in PLGA microspheres showed a sustained release over a period of 2 weeks, whereas rhVEGF165 loaded in hydrogel eluted almost completely from the hydrogel over the first 16 days.
30231646	0	31	theme	dual	23:26	arg1	factor					35:40	dual growth factor	23:40	dual growth factor delivery from composite hydrogels	23:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	5	32	theme	PLGA	796:799	arg1	microspheres					801:812	PLGA microspheres	796:812	PLGA microspheres	796:812	RhBMP-2 loaded in PLGA microspheres showed a sustained release over a period of 2 weeks, whereas rhVEGF165 loaded in hydrogel eluted almost completely from the hydrogel over the first 16 days.
30231646	4	33	theme	growth	732:737	arg1	factors					739:745	the two growth factors	724:745	the two growth factors	724:745	The system showed a similar release behaviour of the two growth factors regardless of 26SCS inclusion.
30231646	6	34	theme	relevant	1035:1042	arg1	assays					1053:1058	relevant in vitro assays	1035:1058	relevant in vitro assays	1035:1058	Both growth factors retained their efficacy, as quantified with relevant in vitro assays.
30231646	7	35	theme	growth	1136:1141	arg1	factors					1143:1149	dual growth factors	1131:1149	dual growth factors	1131:1149	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	1	36	theme	temporal	297:304	arg1	mode					315:318	a controlled spatial and temporal delivery mode	272:318	a controlled spatial and temporal delivery mode	272:318	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	5	37	theme	first	956:960	arg1	16 days					962:968	the first 16 days	952:968	the first 16 days	952:968	RhBMP-2 loaded in PLGA microspheres showed a sustained release over a period of 2 weeks, whereas rhVEGF165 loaded in hydrogel eluted almost completely from the hydrogel over the first 16 days.
30231646	8	38	theme	gene	1419:1422	arg1	expression					1424:1433	angiogenic gene expression	1408:1433	angiogenic gene expression	1408:1433	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	8	39	theme	alkaline	1285:1292	arg1	phosphatase					1294:1304	alkaline phosphatase	1285:1304	significantly upregulated alkaline phosphatase activity	1259:1313	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	5	40	theme	sustained	823:831	arg1	release					833:839	a sustained release	821:839	a sustained release	821:839	RhBMP-2 loaded in PLGA microspheres showed a sustained release over a period of 2 weeks, whereas rhVEGF165 loaded in hydrogel eluted almost completely from the hydrogel over the first 16 days.
30231646	0	41	theme	factor	35:40	arg1	delivery					42:49	dual growth factor delivery	23:49	dual growth factor delivery from composite hydrogels	23:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	1	42	theme	delivery	306:313	arg1	mode					315:318	a controlled spatial and temporal delivery mode	272:318	a controlled spatial and temporal delivery mode	272:318	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	2	43	from	rhVEGF165	492:500	arg1	manner					518:523	the required manner	505:523	the required manner	505:523	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	3	44	theme	synergetic	586:595	arg1	chitosan					557:564	2-N,6-O-sulphated chitosan	539:564	2-N,6-O-sulphated chitosan (26SCS)	539:572	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	3	44	theme	synergetic	586:595	arg1	factor					597:602	a synergetic factor	584:602	a synergetic factor of growth factors	584:620	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	6	45	theme	in	1044:1045	arg1	assays					1053:1058	relevant in vitro assays	1035:1058	relevant in vitro assays	1035:1058	Both growth factors retained their efficacy, as quantified with relevant in vitro assays.
30231646	1	46	theme	certain	221:227	arg1	factors					236:242	certain growth factors	221:242	certain growth factors	221:242	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	0	47	theme	growth	28:33	arg1	factor					35:40	dual growth factor	23:40	dual growth factor delivery from composite hydrogels	23:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	0	48	from	hydrogels	66:74	arg1	delivery					42:49	dual growth factor delivery	23:49	dual growth factor delivery from composite hydrogels	23:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	0	48	from	hydrogels	66:74	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of dual growth factor delivery from composite hydrogels	0:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	7	49	theme	dual	1131:1134	arg1	factors					1143:1149	dual growth factors	1131:1149	dual growth factors	1131:1149	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	1	50	theme	growth	229:234	arg1	factors					236:242	certain growth factors	221:242	certain growth factors	221:242	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	10	51	theme	further	1706:1712	arg1	study					1714:1718	further study	1706:1718	further study	1706:1718	From our findings, we could also conclude that rhBMP-2 can promote angiogenesis and that the mechanism is worthy of further study in subsequent research.
30231646	8	52	theme	nitric	1380:1385	arg1	oxide					1387:1391	nitric oxide	1380:1391	nitric oxide secretion	1380:1401	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	8	53	theme	vein	1332:1335	arg1	proliferation					1354:1366	human umbilical vein endothelial cell proliferation	1316:1366	human umbilical vein endothelial cell proliferation	1316:1366	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	4	54	theme	similar	695:701	arg1	behaviour					711:719	a similar release behaviour	693:719	a similar release behaviour of the two growth factors	693:745	The system showed a similar release behaviour of the two growth factors regardless of 26SCS inclusion.
30231646	0	55	theme	delivery	42:49	arg1	effects					12:18	Synergistic effects	0:18	Synergistic effects of dual growth factor delivery from composite hydrogels	0:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	2	56	theme	osteogenic	458:467	arg1	rhBMP-2					469:475	osteogenic rhBMP-2	458:475	osteogenic rhBMP-2	458:475	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	7	57	theme	enhanced	1074:1081	arg1	response					1088:1095	an enhanced cell response	1071:1095	an enhanced cell response	1071:1095	Moreover, an enhanced cell response was achieved upon the delivery of dual growth factors, compared to that obtained with a single factor.
30231646	9	58	theme	composite	1468:1476	arg1	hydrogel					1478:1485	the composite hydrogel	1464:1485	the composite hydrogel incorporated with 26SCS	1464:1509	This study highlighted that the composite hydrogel incorporated with 26SCS appears to constitute a promising approach to deliver multiple growth factors.
30231646	3	59	theme	factors	614:620	arg1	chitosan					557:564	2-N,6-O-sulphated chitosan	539:564	2-N,6-O-sulphated chitosan (26SCS)	539:572	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	3	59	theme	factors	614:620	arg1	factor					597:602	a synergetic factor	584:602	a synergetic factor of growth factors	584:620	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	1	60	theme	promising	141:149	arg1	strategy					151:158	A promising strategy	139:158	A promising strategy to accelerate bone generation	139:188	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	9	61	theme	multiple	1565:1572	arg1	factors					1581:1587	multiple growth factors	1565:1587	multiple growth factors	1565:1587	This study highlighted that the composite hydrogel incorporated with 26SCS appears to constitute a promising approach to deliver multiple growth factors.
30231646	1	62	theme	factors	236:242	arg1	combination					206:216	a combination	204:216	a combination of certain growth factors	204:242	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
30231646	8	63	theme	human	1316:1320	arg1	proliferation					1354:1366	human umbilical vein endothelial cell proliferation	1316:1366	human umbilical vein endothelial cell proliferation	1316:1366	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	3	64	theme	composite	647:655	arg1	hydrogel					657:664	the composite hydrogel	643:664	the composite hydrogel	643:664	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	8	65	theme	oxide	1387:1391	arg1	secretion					1393:1401	nitric oxide secretion	1380:1401	nitric oxide secretion	1380:1401	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	4	66	theme	release	703:709	arg1	behaviour					711:719	a similar release behaviour	693:719	a similar release behaviour of the two growth factors	693:745	The system showed a similar release behaviour of the two growth factors regardless of 26SCS inclusion.
30231646	2	67	theme	composite	329:337	arg1	hydrogel					339:346	a composite hydrogel	327:346	a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres	327:407	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	2	68	theme	required	509:516	arg1	manner					518:523	the required manner	505:523	the required manner	505:523	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	8	69	theme	upregulated	1273:1283	arg1	activity					1306:1313	significantly upregulated alkaline phosphatase activity	1259:1313	significantly upregulated alkaline phosphatase activity	1259:1313	Furthermore, in the presence of 26SCS, the system revealed significantly upregulated alkaline phosphatase activity, human umbilical vein endothelial cell proliferation, sprouting, nitric oxide secretion, and angiogenic gene expression.
30231646	9	70	theme	growth	1574:1579	arg1	factors					1581:1587	multiple growth factors	1565:1587	multiple growth factors	1565:1587	This study highlighted that the composite hydrogel incorporated with 26SCS appears to constitute a promising approach to deliver multiple growth factors.
30231646	0	71	theme	composite	56:64	arg1	hydrogels					66:74	composite hydrogels	56:74	composite hydrogels	56:74	Synergistic effects of dual growth factor delivery from composite hydrogels incorporating 2-N,6-O-sulphated chitosan on bone regeneration.
30231646	3	72	theme	growth	607:612	arg1	factors					614:620	growth factors	607:620	growth factors	607:620	In addition, 2-N,6-O-sulphated chitosan (26SCS), which is a synergetic factor of growth factors, was incorporated in the composite hydrogel as well.
30231646	9	73	theme	promising	1535:1543	arg1	approach					1545:1552	a promising approach	1533:1552	a promising approach to deliver multiple growth factors	1533:1587	This study highlighted that the composite hydrogel incorporated with 26SCS appears to constitute a promising approach to deliver multiple growth factors.
30231646	2	74	theme	lactide-co-glycolide	367:386	arg1	microspheres					396:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	poly(lactide-co-glycolide) (PLGA) microspheres	362:407	Here, a composite hydrogel incorporating poly(lactide-co-glycolide) (PLGA) microspheres was accordingly prepared to load and deliver the osteogenic rhBMP-2 and angiogenic rhVEGF165 in the required manner.
30231646	1	75	theme	integration	251:261	arg1	site					263:266	the integration site	247:266	the integration site	247:266	A promising strategy to accelerate bone generation is to deliver a combination of certain growth factors to the integration site via a controlled spatial and temporal delivery mode.
31610423	2	0	dep	only	187:190	arg1	Not					183:185	Not	183:185	Not	183:185	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	5	1	theme	oxidative	753:761	arg1	stability					763:771	oxidative stability	753:771	oxidative stability	753:771	As for the measurement of oxidative stability in general, p-AnV was found suitable in depicting oxidation of EC oleogels.
31610423	2	2	from	changes	318:324	arg1	relation					255:262	relation	255:262	relation to textural attributes	255:285	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	1	3	theme	thermo-oxidative	78:93	arg1	behavior					95:102	The thermo-oxidative behavior	74:102	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels	74:163	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels is characterized.
31610423	3	4	theme	gel	516:518	arg1	network					520:526	the gel network	512:526	the gel network	512:526	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	4	5	theme	food	691:694	arg1	systems					696:702	food systems	691:702	food systems	691:702	Processing temperature ≤ 120 °C for <2 h was recommended when incorporated in food systems to minimize oxidation.
31610423	2	6	from	composition	221:231	arg1	relation					255:262	relation	255:262	relation to textural attributes	255:285	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	2	7	theme	gels	381:384	arg1	heating					353:359	heating	353:359	heating	353:359	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	2	7	theme	gels	381:384	arg1	holding					366:372	holding	366:372	holding	366:372	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	2	7	theme	gels	381:384	arg1	formation					342:350	formation	342:350	formation	342:350	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	3	8	theme	oleogel	390:396	arg1	stability					408:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	1	9	theme	crucial	104:110	arg1	behavior					95:102	The thermo-oxidative behavior	74:102	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels	74:163	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels is characterized.
31610423	4	10	theme	Processing	613:622	arg1	temperature ≤ 120 °C					624:643	Processing temperature ≤ 120 °C	613:643	Processing temperature ≤ 120 °C for <2 h	613:652	Processing temperature ≤ 120 °C for <2 h was recommended when incorporated in food systems to minimize oxidation.
31610423	2	11	theme	chemical	309:316	arg1	changes					318:324	the dynamic chemical changes	297:324	the dynamic chemical changes occurred during formation, heating, and holding of the gels	297:384	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	5	12	theme	oleogels	839:846	arg1	oxidation					823:831	oxidation	823:831	oxidation of EC oleogels	823:846	As for the measurement of oxidative stability in general, p-AnV was found suitable in depicting oxidation of EC oleogels.
31610423	6	13	theme	oxidative	930:938	arg1	indicators					940:949	reliable oxidative indicators	921:949	reliable oxidative indicators	921:949	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	6	13	theme	oxidative	930:938	arg1	Meanwhile					849:857	Meanwhile	849:857	Meanwhile	849:857	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	6	14	theme	1H	891:892	arg1	spectra					898:904	1H NMR spectra	891:904	1H NMR spectra	891:904	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	2	15	theme	dynamic	301:307	arg1	changes					318:324	the dynamic chemical changes	297:324	the dynamic chemical changes occurred during formation, heating, and holding of the gels	297:384	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	6	16	theme	total	960:964	arg1	oxidation					966:974	total oxidation	960:974	total oxidation of EC oleogels	960:989	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	0	17	theme	behavior	37:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of thermo-oxidative behavior of ethylcellulose	0:62	Characterization of thermo-oxidative behavior of ethylcellulose oleogels.
31610423	6	18	theme	reliable	921:928	arg1	indicators					940:949	reliable oxidative indicators	921:949	reliable oxidative indicators	921:949	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	6	18	theme	reliable	921:928	arg1	Meanwhile					849:857	Meanwhile	849:857	Meanwhile	849:857	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	3	19	theme	EC100	438:442	arg1	concentration					444:456	6.0% EC100 concentration	433:456	6.0% EC100 concentration in the oleogels	433:472	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	0	20	theme	thermo-oxidative	20:35	arg1	behavior					37:44	thermo-oxidative behavior	20:44	thermo-oxidative behavior of ethylcellulose	20:62	Characterization of thermo-oxidative behavior of ethylcellulose oleogels.
31610423	3	21	theme	liquid	490:495	arg1	oil					497:499	liquid oil	490:499	liquid oil trapped in the gel network	490:526	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	0	22	theme	ethylcellulose	49:62	arg1	behavior					37:44	thermo-oxidative behavior	20:44	thermo-oxidative behavior of ethylcellulose	20:62	Characterization of thermo-oxidative behavior of ethylcellulose oleogels.
31610423	2	23	theme	gel	240:242	arg1	network					244:250	the gel network	236:250	the gel network	236:250	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	6	24	dep	Meanwhile	849:857	arg1	both					860:863	both	860:863	both	860:863	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	3	25	theme	6.0	433:435	arg1	%					436:436	%	436:436	%	436:436	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	3	26	theme	oxidative	398:406	arg1	stability					408:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	5	27	theme	stability	763:771	arg1	measurement					738:748	the measurement	734:748	the measurement of oxidative stability in general	734:782	As for the measurement of oxidative stability in general, p-AnV was found suitable in depicting oxidation of EC oleogels.
31610423	3	28	theme	%	436:436	arg1	concentration					444:456	6.0% EC100 concentration	433:456	6.0% EC100 concentration in the oleogels	433:472	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	3	29	theme	retarding	592:600	arg1	oxidation					602:610	retarding oxidation	592:610	retarding oxidation	592:610	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	1	30	theme	ethylcellulose	136:149	arg1	oleogels					156:163	ethylcellulose (EC) oleogels	136:163	ethylcellulose (EC) oleogels	136:163	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels is characterized.
31610423	3	31	theme	EC	387:388	arg1	stability					408:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability	387:416	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	6	32	theme	NMR	894:896	arg1	spectra					898:904	1H NMR spectra	891:904	1H NMR spectra	891:904	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	5	33	from	measurement	738:748	arg1	general					776:782	general	776:782	general	776:782	As for the measurement of oxidative stability in general, p-AnV was found suitable in depicting oxidation of EC oleogels.
31610423	3	34	theme	high	548:551	arg1	viscosity					553:561	its high viscosity	544:561	its high viscosity	544:561	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	2	35	theme	network	244:250	arg1	composition					221:231	the composition	217:231	the composition of the gel network in relation to textural attributes	217:285	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	2	35	theme	network	244:250	arg1	changes					318:324	the dynamic chemical changes	297:324	the dynamic chemical changes occurred during formation, heating, and holding of the gels	297:384	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	3	36	from	concentration	444:456	arg1	oleogels					465:472	the oleogels	461:472	the oleogels	461:472	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	1	37	theme	EC	152:153	arg1	oleogels					156:163	ethylcellulose (EC) oleogels	136:163	ethylcellulose (EC) oleogels	136:163	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels is characterized.
31610423	6	38	theme	oleogels	982:989	arg1	oxidation					966:974	total oxidation	960:974	total oxidation of EC oleogels	960:989	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	2	39	theme	textural	267:274	arg1	attributes					276:285	textural attributes	267:285	textural attributes	267:285	Not only did we take into account the composition of the gel network in relation to textural attributes, but also the dynamic chemical changes occurred during formation, heating, and holding of the gels.
31610423	6	40	theme	EC	979:980	arg1	oleogels					982:989	EC oleogels	979:989	EC oleogels	979:989	Meanwhile, both Rao and Rad acquired from 1H NMR spectra could serve as reliable oxidative indicators to gauge total oxidation of EC oleogels during storage.
31610423	3	41	theme	stable	567:572	arg1	network					578:584	stable gel network	567:584	stable gel network	567:584	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	1	42	theme	oleogels	156:163	arg1	applicability					119:131	the applicability	115:131	the applicability of ethylcellulose (EC) oleogels	115:163	The thermo-oxidative behavior crucial to the applicability of ethylcellulose (EC) oleogels is characterized.
31610423	5	43	theme	EC	836:837	arg1	oleogels					839:846	EC oleogels	836:846	EC oleogels	836:846	As for the measurement of oxidative stability in general, p-AnV was found suitable in depicting oxidation of EC oleogels.
31610423	3	44	theme	gel	574:576	arg1	network					578:584	stable gel network	567:584	stable gel network	567:584	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31610423	3	45	theme	oil	497:499	arg1	movement					478:485	the movement	474:485	the movement of liquid oil trapped in the gel network	474:526	EC oleogel oxidative stability showed that at 6.0% EC100 concentration in the oleogels the movement of liquid oil trapped in the gel network was hindered by its high viscosity and stable gel network, thus retarding oxidation.
31951998	0	0	theme	electrochemical	94:108	arg1	sensor					110:115	high-performance electrochemical sensor	77:115	high-performance electrochemical sensor	77:115	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	8	1	theme	low	1213:1215	arg1	limit					1227:1231	a low detection limit	1211:1231	a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM)	1211:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	7	2	theme	blood	1089:1093	arg1	samples					1101:1107	blood serum samples	1089:1107	blood serum samples with high specificity	1089:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	4	3	theme	CuFe2O4	735:741	arg1	nanomaterial					743:754	CuFe2O4 nanomaterial	735:754	CuFe2O4 nanomaterial	735:754	In this work, ultrasonic bath was used to synthesis of CuFe2O4 nanomaterial at 40 kHz with 1 h. CuFe2O4/chitosan was characterized by FESEM, EDX, XRD and electrochemical methods.
31951998	8	4	theme	CuFe2O4/chitosan	1162:1177	arg1	electrode					1188:1196	The CuFe2O4/chitosan modified electrode is	1158:1199	The CuFe2O4/chitosan modified electrode is	1158:1199	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	0	5	theme	high-performance	77:92	arg1	sensor					110:115	high-performance electrochemical sensor	77:115	high-performance electrochemical sensor	77:115	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	7	6	theme	of	1134:1135	arg1	importance					1146:1155	of greatest importance	1134:1155	of greatest importance	1134:1155	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	7	6	theme	of	1134:1135	arg1	measurement					1054:1064	the measurement	1050:1064	the measurement of 8-hydroxyguanine in blood serum samples with high specificity	1050:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	1	7	theme	CuFe2O4	173:179	arg1	nanoparticles					158:170	copper ferrite nanoparticles	143:170	copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	143:193	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	1	7	theme	CuFe2O4	173:179	arg1	NPs					181:183	CuFe2O4 NPs	173:183	CuFe2O4 NPs	173:183	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	6	8	theme	8-hydroxyguanine	952:967	arg1	concentrations					934:947	The concentrations	930:947	The concentrations of 8-hydroxyguanine within a cell	930:981	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	6	8	theme	8-hydroxyguanine	952:967	arg1	measurement					989:999	a measurement	987:999	a measurement of oxidative stress in human body	987:1033	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	2	9	theme	high	292:295	arg1	sensor					343:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	3	10	dep	synthesis	560:568	arg1	structure					587:595	structure	587:595	structure	587:595	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	8	11	theme	detection	1217:1225	arg1	limit					1227:1231	a low detection limit	1211:1231	a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM)	1211:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	7	12	theme	8-hydroxyguanine	1069:1084	arg1	importance					1146:1155	of greatest importance	1134:1155	of greatest importance	1134:1155	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	7	12	theme	8-hydroxyguanine	1069:1084	arg1	measurement					1054:1064	the measurement	1050:1064	the measurement of 8-hydroxyguanine in blood serum samples with high specificity	1050:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	4	13	dep	FESEM	814:818	arg1	methods					850:856	methods	850:856	methods	850:856	In this work, ultrasonic bath was used to synthesis of CuFe2O4 nanomaterial at 40 kHz with 1 h. CuFe2O4/chitosan was characterized by FESEM, EDX, XRD and electrochemical methods.
31951998	2	14	theme	electrochemical	327:341	arg1	sensor					343:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	8	15	theme	8.6 nM	1236:1241	arg1	limit					1227:1231	a low detection limit	1211:1231	a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM)	1211:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	7	16	from	measurement	1054:1064	arg1	samples					1101:1107	blood serum samples	1089:1107	blood serum samples with high specificity	1089:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	1	17	theme	/chitosan	185:193	arg1	synthesis					130:138	the synthesis	126:138	the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	126:193	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	5	18	theme	biomarker	899:907	arg1	one					892:894	one	892:894	one	892:894	Furthermore, 8-hydroxyguanine is one of biomarker by oxidative stress.
31951998	5	18	theme	biomarker	899:907	arg1	biomarker					899:907	biomarker	899:907	biomarker	899:907	Furthermore, 8-hydroxyguanine is one of biomarker by oxidative stress.
31951998	2	19	theme	nanoparticles	402:414	arg1	composition					372:382	a composition	370:382	a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach	370:479	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	8	20	theme	long	1247:1250	arg1	0.025-697.175 µM					1266:1281	0.025-697.175 µM	1266:1281	0.025-697.175 µM	1266:1281	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	8	20	theme	long	1247:1250	arg1	range					1259:1263	long linear range	1247:1263	long linear range (0.025-697.175 µM)	1247:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	1	21	from	50 W	284:287	arg1	bath					265:268	ultrasonic irradiation bath	242:268	ultrasonic irradiation bath at 40 kHz and 50 W	242:287	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	2	22	theme	modified	318:325	arg1	sensor					343:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	2	23	theme	ferrite	394:400	arg1	nanoparticles					402:414	copper ferrite nanoparticles	387:414	copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach	387:479	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	0	24	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of copper ferrite	0:33	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	6	25	theme	human	1024:1028	arg1	body					1030:1033	human body	1024:1033	human body	1024:1033	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	7	26	theme	greatest	1137:1144	arg1	importance					1146:1155	of greatest importance	1134:1155	of greatest importance	1134:1155	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	7	26	theme	greatest	1137:1144	arg1	measurement					1054:1064	the measurement	1050:1064	the measurement of 8-hydroxyguanine in blood serum samples with high specificity	1050:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	2	27	theme	stable	311:316	arg1	sensor					343:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	2	28	theme	copper	387:392	arg1	nanoparticles					402:414	copper ferrite nanoparticles	387:414	copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach	387:479	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	0	29	theme	copper	20:25	arg1	ferrite					27:33	copper ferrite	20:33	copper ferrite	20:33	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	3	30	dep	power	491:495	arg1	parameters					511:520	parameters	511:520	parameters	511:520	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	1	31	from	40 kHz	273:278	arg1	bath					265:268	ultrasonic irradiation bath	242:268	ultrasonic irradiation bath at 40 kHz and 50 W	242:287	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	8	32	theme	range	1259:1263	arg1	limit					1227:1231	a low detection limit	1211:1231	a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM)	1211:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	2	33	theme	facile	454:459	arg1	approach					472:479	a facile ultrasound approach	452:479	a facile ultrasound approach	452:479	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	6	34	theme	stress	1014:1019	arg1	measurement					989:999	a measurement	987:999	a measurement of oxidative stress in human body	987:1033	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	6	34	theme	stress	1014:1019	arg1	concentrations					934:947	The concentrations	930:947	The concentrations of 8-hydroxyguanine within a cell	930:981	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	2	35	theme	sensitive	297:305	arg1	sensor					343:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor	290:348	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	3	36	theme	sonochemical	547:558	arg1	synthesis					560:568	synthesis	560:568	synthesis	560:568	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	6	37	theme	oxidative	1004:1012	arg1	stress					1014:1019	oxidative stress	1004:1019	oxidative stress	1004:1019	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	7	38	with	samples	1101:1107	arg1	specificity					1119:1129	high specificity	1114:1129	high specificity	1114:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	0	39	theme	ferrite	27:33	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of copper ferrite	0:33	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	5	40	theme	oxidative	912:920	arg1	stress					922:927	oxidative stress	912:927	oxidative stress	912:927	Furthermore, 8-hydroxyguanine is one of biomarker by oxidative stress.
31951998	2	41	theme	ultrasound	461:470	arg1	approach					472:479	a facile ultrasound approach	452:479	a facile ultrasound approach	452:479	A high sensitive and stable modified electrochemical sensor was developed using a composition of copper ferrite nanoparticles coordinated with biopolymer through a facile ultrasound approach.
31951998	1	42	theme	sonochemical	217:228	arg1	route					230:234	sonochemical route	217:234	sonochemical route	217:234	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	7	43	theme	serum	1095:1099	arg1	samples					1101:1107	blood serum samples	1089:1107	blood serum samples with high specificity	1089:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	3	44	theme	nanomaterials	614:626	arg1	development					628:638	the nanomaterials development	610:638	the nanomaterials development	610:638	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	4	45	used	used	714:717	arg2	bath					705:708	ultrasonic bath	694:708	ultrasonic bath	694:708	In this work, ultrasonic bath was used to synthesis of CuFe2O4 nanomaterial at 40 kHz with 1 h. CuFe2O4/chitosan was characterized by FESEM, EDX, XRD and electrochemical methods.
31951998	7	46	theme	high	1114:1117	arg1	specificity					1119:1129	high specificity	1114:1129	high specificity	1114:1129	Consequently, the measurement of 8-hydroxyguanine in blood serum samples with high specificity is of greatest importance.
31951998	3	47	theme	development	628:638	arg1	size					602:605	size	602:605	size	602:605	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	3	47	theme	development	628:638	arg1	synthesis					560:568	synthesis	560:568	synthesis	560:568	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	4	48	theme	nanomaterial	743:754	arg1	synthesis					722:730	synthesis	722:730	synthesis of CuFe2O4 nanomaterial	722:754	In this work, ultrasonic bath was used to synthesis of CuFe2O4 nanomaterial at 40 kHz with 1 h. CuFe2O4/chitosan was characterized by FESEM, EDX, XRD and electrochemical methods.
31951998	8	49	theme	modified	1179:1186	arg1	electrode					1188:1196	The CuFe2O4/chitosan modified electrode is	1158:1199	The CuFe2O4/chitosan modified electrode is	1158:1199	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	8	50	theme	linear	1252:1257	arg1	0.025-697.175 µM					1266:1281	0.025-697.175 µM	1266:1281	0.025-697.175 µM	1266:1281	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	8	50	theme	linear	1252:1257	arg1	range					1259:1263	long linear range	1247:1263	long linear range (0.025-697.175 µM)	1247:1282	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	6	51	from	measurement	989:999	arg1	body					1030:1033	human body	1024:1033	human body	1024:1033	The concentrations of 8-hydroxyguanine within a cell are a measurement of oxidative stress in human body.
31951998	1	52	theme	copper	143:148	arg1	nanoparticles					158:170	copper ferrite nanoparticles	143:170	copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	143:193	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	1	52	theme	copper	143:148	arg1	NPs					181:183	CuFe2O4 NPs	173:183	CuFe2O4 NPs	173:183	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	3	53	theme	ultrasonic	651:660	arg1	time					674:677	the ultrasonic irradiation time	647:677	the ultrasonic irradiation time	647:677	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	1	54	theme	ferrite	150:156	arg1	nanoparticles					158:170	copper ferrite nanoparticles	143:170	copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	143:193	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	1	54	theme	ferrite	150:156	arg1	NPs					181:183	CuFe2O4 NPs	173:183	CuFe2O4 NPs	173:183	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	4	55	theme	ultrasonic	694:703	arg1	bath					705:708	ultrasonic bath	694:708	ultrasonic bath	694:708	In this work, ultrasonic bath was used to synthesis of CuFe2O4 nanomaterial at 40 kHz with 1 h. CuFe2O4/chitosan was characterized by FESEM, EDX, XRD and electrochemical methods.
31951998	1	56	theme	ultrasonic	242:251	arg1	bath					265:268	ultrasonic irradiation bath	242:268	ultrasonic irradiation bath at 40 kHz and 50 W	242:287	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	0	57	theme	composite	63:71	arg1	chitosan					54:61	chitosan composite	54:71	chitosan composite for high-performance electrochemical sensor	54:115	Facile synthesis of copper ferrite nanoparticles with chitosan composite for high-performance electrochemical sensor.
31951998	3	58	theme	irradiation	662:672	arg1	time					674:677	the ultrasonic irradiation time	647:677	the ultrasonic irradiation time	647:677	Besides, power and frequency parameters are highly important for sonochemical synthesis and specifically structure, and size of the nanomaterials development during the ultrasonic irradiation time.
31951998	1	59	theme	nanoparticles	158:170	arg1	/chitosan					185:193	copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	143:193	copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan	143:193	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
31951998	8	60	dep	electrode	1188:1196	arg1	is					1198:1199	is	1198:1199	is	1198:1199	The CuFe2O4/chitosan modified electrode is displayed a low detection limit of 8.6 nM and long linear range (0.025-697.175 µM).
31951998	1	61	theme	irradiation	253:263	arg1	bath					265:268	ultrasonic irradiation bath	242:268	ultrasonic irradiation bath at 40 kHz and 50 W	242:287	Herein, the synthesis of copper ferrite nanoparticles (CuFe2O4 NPs)/chitosan have been prepared by sonochemical route under ultrasonic irradiation bath at 40 kHz and 50 W.
29499264	0	0	theme	response	70:77	arg1	methodology					79:89	surface response methodology	62:89	surface response methodology	62:89	Optimization of pectin extraction from Ubá mango peel through surface response methodology.
29499264	1	1	theme	pH	152:153	arg1	effects					141:147	effects	141:147	effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted	141:308	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	6	2	theme	desirability	873:884	arg1	function					886:893	the desirability function	869:893	the desirability function	869:893	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	6	3	dep	85.4°C<T<97°C	951:963	arg1	1.66<pH<1.79					1012:1023	1.66<pH<1.79	1012:1023	1.66<pH<1.79	1012:1023	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	6	3	dep	85.4°C<T<97°C	951:963	arg1	94.3°C<T<97°C					997:1009	94.3°C<T<97°C	997:1009	94.3°C<T<97°C	997:1009	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	6	3	dep	85.4°C<T<97°C	951:963	arg1	80min<t<85min					982:994	80min<t<85min	982:994	80min<t<85min	982:994	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	6	3	dep	85.4°C<T<97°C	951:963	arg1	1.66<pH<2.4					966:976	1.66<pH<2.4	966:976	1.66<pH<2.4	966:976	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	2	4	theme	ethanol	440:446	arg1	solution					448:455	an ethanol solution	437:455	an ethanol solution obtaining the alcohol insoluble residue (AIR)	437:501	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	1	5	theme	pectins	292:298	arg1	degree					235:240	degree	235:240	degree of degree of esterification (DE)	235:273	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	5	theme	pectins	292:298	arg1	yield					228:232	the yield	224:232	the yield	224:232	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	5	theme	pectins	292:298	arg1	viscosity					279:287	viscosity	279:287	viscosity	279:287	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	2	6	theme	sanitized	349:357	arg1	dried					377:381	dried	377:381	dried	377:381	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	2	6	theme	sanitized	349:357	arg1	shells					365:370	the previously sanitized mango shells	334:370	the previously sanitized mango shells	334:370	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	4	7	theme	esterification	673:686	arg1	degree					663:668	the degree	659:668	the degree of esterification (DE) and viscosity values	659:712	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	4	8	dep	45.85mPa·s	757:766	arg1	to					754:755	to	754:755	to	754:755	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	4	9	theme	AIR	646:648	arg1	AIR					646:648	AIR	646:648	AIR	646:648	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	4	9	theme	AIR	646:648	arg1	32.1g/100g					632:641	32.1g/100g	632:641	32.1g/100g	632:641	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	0	10	from	mango	43:47	arg1	Optimization					0:11	Optimization	0:11	Optimization of pectin extraction from Ubá mango	0:47	Optimization of pectin extraction from Ubá mango peel through surface response methodology.
29499264	2	11	theme	pectin	315:320	arg1	extraction					322:331	pectin extraction	315:331	pectin extraction	315:331	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	4	12	dep	86.2	734:737	arg1	to					731:732	to	731:732	to	731:732	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	1	13	used	used	123:126	arg2	design					112:117	A central composite design	92:117	A central composite design	92:117	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	0	14	theme	extraction	23:32	arg1	Optimization					0:11	Optimization	0:11	Optimization of pectin extraction from Ubá mango	0:47	Optimization of pectin extraction from Ubá mango peel through surface response methodology.
29499264	2	15	theme	insoluble	479:487	arg1	AIR					498:500	AIR	498:500	AIR	498:500	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	2	15	theme	insoluble	479:487	arg1	residue					489:495	the alcohol insoluble residue	467:495	the alcohol insoluble residue (AIR)	467:501	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	1	16	from	effects	141:147	arg1	degree					235:240	degree	235:240	degree of degree of esterification (DE)	235:273	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	16	from	effects	141:147	arg1	yield					228:232	the yield	224:232	the yield	224:232	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	16	from	effects	141:147	arg1	viscosity					279:287	viscosity	279:287	viscosity	279:287	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	17	theme	central	94:100	arg1	design					112:117	A central composite design	92:117	A central composite design	92:117	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	2	18	theme	mango	359:363	arg1	dried					377:381	dried	377:381	dried	377:381	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	2	18	theme	mango	359:363	arg1	shells					365:370	the previously sanitized mango shells	334:370	the previously sanitized mango shells	334:370	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	0	19	theme	pectin	16:21	arg1	extraction					23:32	pectin extraction	16:32	pectin extraction	16:32	Optimization of pectin extraction from Ubá mango peel through surface response methodology.
29499264	2	20	theme	alcohol	471:477	arg1	AIR					498:500	AIR	498:500	AIR	498:500	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	2	20	theme	alcohol	471:477	arg1	residue					489:495	the alcohol insoluble residue	467:495	the alcohol insoluble residue (AIR)	467:501	For pectin extraction, the previously sanitized mango shells were dried and crushed to obtain the flour that was treated with an ethanol solution obtaining the alcohol insoluble residue (AIR).
29499264	7	21	from	AIR	1071:1073	arg1	%					1060:1060	>30%	1057:1060	>30% from the AIR	1057:1073	Under these conditions, yields >30% from the AIR, DE≥70.0% and η≥20.0mPa·s.
29499264	7	21	from	AIR	1071:1073	arg1	%					1083:1083	DE≥70.0%	1076:1083	DE≥70.0%	1076:1083	Under these conditions, yields >30% from the AIR, DE≥70.0% and η≥20.0mPa·s.
29499264	7	21	from	AIR	1071:1073	arg1	η≥20.0mPa·s					1089:1099	η≥20.0mPa·s	1089:1099	η≥20.0mPa·s	1089:1099	Under these conditions, yields >30% from the AIR, DE≥70.0% and η≥20.0mPa·s.
29499264	1	22	theme	composite	102:110	arg1	design					112:117	A central composite design	92:117	A central composite design	92:117	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	23	theme	temperature	179:189	arg1	effects					141:147	effects	141:147	effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted	141:308	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	3	24	theme	extraction	556:565	arg1	solution					567:574	the extraction solution	552:574	the extraction solution of hydrochloric acid	552:595	Subsequently, the AIR was mixed in ethanol with the extraction solution of hydrochloric acid.
29499264	4	25	theme	values	707:712	arg1	degree					663:668	the degree	659:668	the degree of esterification (DE) and viscosity values	659:712	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	1	26	theme	degree	245:250	arg1	degree					235:240	degree	235:240	degree of degree of esterification (DE)	235:273	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	26	theme	degree	245:250	arg1	yield					228:232	the yield	224:232	the yield	224:232	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	26	theme	degree	245:250	arg1	viscosity					279:287	viscosity	279:287	viscosity	279:287	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	4	27	theme	viscosity	697:705	arg1	values					707:712	viscosity values	697:712	viscosity values	697:712	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	1	28	theme	esterification	255:268	arg1	degree					245:250	degree	245:250	degree of esterification (DE)	245:273	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	3	29	theme	hydrochloric	579:590	arg1	acid					592:595	hydrochloric acid	579:595	hydrochloric acid	579:595	Subsequently, the AIR was mixed in ethanol with the extraction solution of hydrochloric acid.
29499264	5	30	theme	inverse	786:792	arg1	correlation					794:804	An inverse correlation	783:804	An inverse correlation	783:804	An inverse correlation was found between extraction yield and viscosity.
29499264	3	31	theme	acid	592:595	arg1	solution					567:574	the extraction solution	552:574	the extraction solution of hydrochloric acid	552:595	Subsequently, the AIR was mixed in ethanol with the extraction solution of hydrochloric acid.
29499264	4	32	dep	32.1g/100g	632:641	arg1	to					629:630	to	629:630	to	629:630	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	5	33	theme	extraction	824:833	arg1	yield					835:839	extraction yield	824:839	extraction yield	824:839	An inverse correlation was found between extraction yield and viscosity.
29499264	6	34	theme	optimum	900:906	arg1	conditions					908:917	two optimum conditions	896:917	two optimum conditions	896:917	Relying upon the desirability function, two optimum conditions were determined: 35min<t<48min; 85.4°C<T<97°C; 1.66<pH<2.4 and 80min<t<85min; 94.3°C<T<97°C; 1.66<pH<1.79.
29499264	0	35	theme	surface	62:68	arg1	methodology					79:89	surface response methodology	62:89	surface response methodology	62:89	Optimization of pectin extraction from Ubá mango peel through surface response methodology.
29499264	1	36	theme	extraction	168:177	arg1	temperature					179:189	extraction temperature	168:189	extraction temperature (63-97°C)	168:199	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	1	36	theme	extraction	168:177	arg1	63-97°C					192:198	63-97°C	192:198	63-97°C	192:198	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29499264	4	37	theme	Pectin	598:603	arg1	yields					605:610	Pectin yields	598:610	Pectin yields	598:610	Pectin yields ranged from 18.8 to 32.1g/100g of AIR, whereas the degree of esterification (DE) and viscosity values ranged from 62.2 to 86.2% and from 1.58 to 45.85mPa·s, respectively.
29499264	1	38	theme	time	205:208	arg1	effects					141:147	effects	141:147	effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted	141:308	A central composite design was used to determine effects of pH (1.16-2.84), extraction temperature (63-97°C) and time (35-85min) on the yield, degree of degree of esterification (DE) and viscosity of pectins extracted.
29677563	3	0	from	colitis	611:617	arg1	mice					630:633	C57BL/6 mice	622:633	C57BL/6 mice	622:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	6	1	theme	alpha	1197:1201	arg1	phosphorylation					1131:1145	phosphorylation	1131:1145	phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt)	1131:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	0	2	theme	microbiota	190:199	arg1	composition					167:177	the composition	163:177	the composition of the gut microbiota	163:199	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	2	3	theme	Chinese	342:348	arg1	erinaceus					312:320	Hericium erinaceus	303:320	Hericium erinaceus (H. erinaceus)	303:335	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	3	theme	Chinese	342:348	arg1	fungus					360:365	a Chinese medicinal fungus	340:365	a Chinese medicinal fungus	340:365	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	6	4	theme	kinase	1265:1270	arg1	Akt					1275:1277	Akt	1275:1277	Akt	1275:1277	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	4	theme	kinase	1265:1270	arg1	B					1272:1272	Protein kinase B	1257:1272	Protein kinase B (Akt)	1257:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	5	5	theme	nitric	1032:1037	arg1	synthase					1045:1052	inducible nitric oxide synthase	1022:1052	inducible nitric oxide synthase (iNOS)	1022:1059	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	5	theme	nitric	1032:1037	arg1	cyclooxygenase-2					996:1011	cyclooxygenase-2	996:1011	cyclooxygenase-2 (COX-2)	996:1019	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	5	theme	nitric	1032:1037	arg1	iNOS					1055:1058	iNOS	1055:1058	iNOS	1055:1058	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	6	6	theme	inhibitor	1187:1195	arg1	alpha					1197:1201	NF-κB inhibitor alpha	1181:1201	NF-κB inhibitor alpha (IκB-α)	1181:1209	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	6	theme	inhibitor	1187:1195	arg1	IκB-α					1204:1208	IκB-α	1204:1208	IκB-α	1204:1208	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	3	7	theme	C57BL/6	622:628	arg1	mice					630:633	C57BL/6 mice	622:633	C57BL/6 mice	622:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	0	8	theme	gut	186:188	arg1	microbiota					190:199	the gut microbiota	182:199	the gut microbiota	182:199	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	6	9	theme	Protein	1257:1263	arg1	Akt					1275:1277	Akt	1275:1277	Akt	1275:1277	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	9	theme	Protein	1257:1263	arg1	B					1272:1272	Protein kinase B	1257:1272	Protein kinase B (Akt)	1257:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	5	10	theme	oxide	1039:1043	arg1	synthase					1045:1052	inducible nitric oxide synthase	1022:1052	inducible nitric oxide synthase (iNOS)	1022:1059	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	10	theme	oxide	1039:1043	arg1	cyclooxygenase-2					996:1011	cyclooxygenase-2	996:1011	cyclooxygenase-2 (COX-2)	996:1019	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	10	theme	oxide	1039:1043	arg1	iNOS					1055:1058	iNOS	1055:1058	iNOS	1055:1058	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	0	11	theme	stress	100:105	arg1	regulation					76:85	regulation	76:85	regulation of oxidative stress	76:105	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	3	12	theme	erinaceus	526:534	arg1	polysaccharide					505:518	polysaccharide	505:518	polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa)	505:556	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	5	13	theme	related	1093:1099	arg1	mRNA					1101:1104	related mRNA	1093:1104	related mRNA	1093:1104	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	7	14	theme	gut	1337:1339	arg1	dysbiosis					1341:1349	DSS-induced gut dysbiosis	1325:1349	DSS-induced gut dysbiosis	1325:1349	Moreover, HECP reversed DSS-induced gut dysbiosis and maintained intestinal barrier integrity.
29677563	0	15	theme	oxidative	90:98	arg1	stress					100:105	oxidative stress	90:105	oxidative stress	90:105	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	5	16	theme	necrosis	950:957	arg1	TNF					967:969	TNF	967:969	TNF	967:969	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	16	theme	necrosis	950:957	arg1	interleukin					903:913	interleukin (IL)-6	903:920	interleukin (IL)-6	903:920	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	16	theme	necrosis	950:957	arg1	factor					959:964	tumor necrosis factor (TNF)-α	944:972	tumor necrosis factor (TNF)-α	944:972	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	3	17	theme	anti-inflammatory	477:493	arg1	effect					495:500	the anti-inflammatory effect	473:500	the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa)	473:556	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	8	18	theme	intestinal	1440:1449	arg1	injury					1451:1456	DSS-induced intestinal injury	1428:1456	DSS-induced intestinal injury	1428:1456	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	0	19	theme	inflammation-related	108:127	arg1	pathways					139:146	inflammation-related signaling pathways	108:146	inflammation-related signaling pathways	108:146	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	3	20	from	model	565:569	arg1	mice					630:633	C57BL/6 mice	622:633	C57BL/6 mice	622:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	0	21	from	colitis	48:54	arg1	mice					67:70	C57BL/6 mice	59:70	C57BL/6 mice	59:70	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	8	22	theme	DSS-induced	1428:1438	arg1	injury					1451:1456	DSS-induced intestinal injury	1428:1456	DSS-induced intestinal injury	1428:1456	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	3	23	theme	H.	523:524	arg1	erinaceus					526:534	H. erinaceus	523:534	H. erinaceus (HECP, Mw: 86.67 kDa)	523:556	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	3	23	theme	H.	523:524	arg1	HECP					537:540	HECP	537:540	HECP	537:540	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	8	24	theme	protective	1507:1516	arg1	nutrient					1526:1533	a protective dietary nutrient	1505:1533	a protective dietary nutrient against IBD	1505:1545	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	8	24	theme	protective	1507:1516	arg1	HECP					1487:1490	HECP	1487:1490	HECP	1487:1490	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	6	25	theme	p65	1176:1178	arg1	phosphorylation					1131:1145	phosphorylation	1131:1145	phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt)	1131:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	3	26	theme	polysaccharide	505:518	arg1	effect					495:500	the anti-inflammatory effect	473:500	the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa)	473:556	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	2	27	theme	disorders	432:440	arg1	prevention					387:396	prevention	387:396	prevention	387:396	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	27	theme	disorders	432:440	arg1	treatment					402:410	treatment	402:410	treatment	402:410	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	1	28	with	immune	273:278	arg1	etiology					293:300	unknown etiology	285:300	unknown etiology	285:300	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	1	29	theme	Inflammatory	202:213	arg1	disease					221:227	Inflammatory bowel disease	202:227	Inflammatory bowel disease (IBD)	202:233	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	1	29	theme	Inflammatory	202:213	arg1	disease					240:246	a disease	238:246	a disease caused by a dysregulated immune with unknown etiology	238:300	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	1	29	theme	Inflammatory	202:213	arg1	IBD					230:232	IBD	230:232	IBD	230:232	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	0	30	theme	erinaceus	27:35	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide of Hericium erinaceus	0:35	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	6	31	theme	NF-κB	1169:1173	arg1	p65					1176:1178	nuclear factor-κB (NF-κB) p65	1150:1178	nuclear factor-κB (NF-κB) p65	1150:1178	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	2	32	theme	Hericium	303:310	arg1	erinaceus					312:320	Hericium erinaceus	303:320	Hericium erinaceus (H. erinaceus)	303:335	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	32	theme	Hericium	303:310	arg1	erinaceus					326:334	H. erinaceus	323:334	H. erinaceus	323:334	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	32	theme	Hericium	303:310	arg1	fungus					360:365	a Chinese medicinal fungus	340:365	a Chinese medicinal fungus	340:365	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	8	33	theme	dietary	1518:1524	arg1	nutrient					1526:1533	a protective dietary nutrient	1505:1533	a protective dietary nutrient against IBD	1505:1545	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	8	33	theme	dietary	1518:1524	arg1	HECP					1487:1490	HECP	1487:1490	HECP	1487:1490	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	2	34	theme	treatment	402:410	arg1	effect					377:382	the effect	373:382	the effect of prevention and treatment of gastrointestinal disorders	373:440	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	0	35	theme	Hericium	18:25	arg1	erinaceus					27:35	Hericium erinaceus	18:35	Hericium erinaceus	18:35	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	1	36	theme	unknown	285:291	arg1	etiology					293:300	unknown etiology	285:300	unknown etiology	285:300	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	0	37	theme	signaling	129:137	arg1	pathways					139:146	inflammation-related signaling pathways	108:146	inflammation-related signaling pathways	108:146	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	6	38	theme	mitogen-activated	1212:1228	arg1	MAPK					1247:1250	MAPK	1247:1250	MAPK	1247:1250	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	38	theme	mitogen-activated	1212:1228	arg1	kinases					1238:1244	mitogen-activated protein kinases	1212:1244	mitogen-activated protein kinases (MAPK)	1212:1251	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	4	39	theme	clinical	679:686	arg1	symptoms					688:695	clinical symptoms	679:695	clinical symptoms	679:695	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	6	40	theme	protein	1230:1236	arg1	MAPK					1247:1250	MAPK	1247:1250	MAPK	1247:1250	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	40	theme	protein	1230:1236	arg1	kinases					1238:1244	mitogen-activated protein kinases	1212:1244	mitogen-activated protein kinases (MAPK)	1212:1251	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	5	41	theme	inducible	1022:1030	arg1	synthase					1045:1052	inducible nitric oxide synthase	1022:1052	inducible nitric oxide synthase (iNOS)	1022:1059	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	41	theme	inducible	1022:1030	arg1	cyclooxygenase-2					996:1011	cyclooxygenase-2	996:1011	cyclooxygenase-2 (COX-2)	996:1019	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	41	theme	inducible	1022:1030	arg1	iNOS					1055:1058	iNOS	1055:1058	iNOS	1055:1058	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	6	42	theme	factor-κB	1158:1166	arg1	p65					1176:1178	nuclear factor-κB (NF-κB) p65	1150:1178	nuclear factor-κB (NF-κB) p65	1150:1178	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	7	43	theme	DSS-induced	1325:1335	arg1	dysbiosis					1341:1349	DSS-induced gut dysbiosis	1325:1349	DSS-induced gut dysbiosis	1325:1349	Moreover, HECP reversed DSS-induced gut dysbiosis and maintained intestinal barrier integrity.
29677563	4	44	theme	nitric	760:765	arg1	NO					774:775	NO	774:775	NO	774:775	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	44	theme	nitric	760:765	arg1	oxide					767:771	nitric oxide	760:771	nitric oxide (NO)	760:776	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	6	45	theme	DSS-treated	1283:1293	arg1	mice					1295:1298	DSS-treated mice	1283:1298	DSS-treated mice	1283:1298	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	2	46	theme	prevention	387:396	arg1	effect					377:382	the effect	373:382	the effect of prevention and treatment of gastrointestinal disorders	373:440	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	3	47	from	mice	630:633	arg1	model					565:569	the model	561:569	the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice	561:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	6	48	theme	nuclear	1150:1156	arg1	p65					1176:1178	nuclear factor-κB (NF-κB) p65	1150:1178	nuclear factor-κB (NF-κB) p65	1150:1178	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	2	49	theme	gastrointestinal	415:430	arg1	disorders					432:440	gastrointestinal disorders	415:440	gastrointestinal disorders	415:440	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	0	50	theme	C57BL/6	59:65	arg1	mice					67:70	C57BL/6 mice	59:70	C57BL/6 mice	59:70	Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota.
29677563	6	51	theme	NF-κB	1181:1185	arg1	alpha					1197:1201	NF-κB inhibitor alpha	1181:1201	NF-κB inhibitor alpha (IκB-α)	1181:1209	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	6	51	theme	NF-κB	1181:1185	arg1	IκB-α					1204:1208	IκB-α	1204:1208	IκB-α	1204:1208	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	4	52	theme	superoxide	808:817	arg1	dismutase					819:827	total superoxide dismutase	802:827	total superoxide dismutase (T-SOD)	802:835	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	52	theme	superoxide	808:817	arg1	T-SOD					830:834	T-SOD	830:834	T-SOD	830:834	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	5	53	theme	tumor	944:948	arg1	TNF					967:969	TNF	967:969	TNF	967:969	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	53	theme	tumor	944:948	arg1	interleukin					903:913	interleukin (IL)-6	903:920	interleukin (IL)-6	903:920	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	53	theme	tumor	944:948	arg1	factor					959:964	tumor necrosis factor (TNF)-α	944:972	tumor necrosis factor (TNF)-α	944:972	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	54	theme	cyclooxygenase-2	996:1011	arg1	expression					982:991	the expression	978:991	the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS)	978:1059	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	54	theme	cyclooxygenase-2	996:1011	arg1	secretion					890:898	the secretion	886:898	the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α	886:972	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	4	55	theme	total	802:806	arg1	dismutase					819:827	total superoxide dismutase	802:827	total superoxide dismutase (T-SOD)	802:835	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	55	theme	total	802:806	arg1	T-SOD					830:834	T-SOD	830:834	T-SOD	830:834	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	3	56	theme	colitis	611:617	arg1	model					565:569	the model	561:569	the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice	561:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	5	57	theme	mRNA	1101:1104	arg1	expression					1079:1088	the expression	1075:1088	the expression of related mRNA	1075:1104	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	58	theme	interleukin	903:913	arg1	expression					982:991	the expression	978:991	the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS)	978:1059	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	5	58	theme	interleukin	903:913	arg1	secretion					890:898	the secretion	886:898	the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α	886:972	HECP also suppressed the secretion of interleukin (IL)-6, interleukin (IL)-1β, tumor necrosis factor (TNF)-α and the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and decreased the expression of related mRNA.
29677563	4	59	theme	oxidative	730:738	arg1	stresses					740:747	oxidative stresses	730:747	oxidative stresses	730:747	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	59	theme	oxidative	730:738	arg1	dismutase					819:827	total superoxide dismutase	802:827	total superoxide dismutase (T-SOD)	802:835	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	59	theme	oxidative	730:738	arg1	myeloperoxidase					842:856	myeloperoxidase	842:856	myeloperoxidase (MPO)	842:862	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	59	theme	oxidative	730:738	arg1	malondialdehyde					779:793	malondialdehyde	779:793	malondialdehyde (MDA)	779:799	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	59	theme	oxidative	730:738	arg1	oxide					767:771	nitric oxide	760:771	nitric oxide (NO)	760:776	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	4	60	theme	key	715:717	arg1	markers					719:725	key markers	715:725	key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO)	715:862	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	2	61	with	fungus	360:365	arg1	effect					377:382	the effect	373:382	the effect of prevention and treatment of gastrointestinal disorders	373:440	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	62	theme	H.	323:324	arg1	erinaceus					312:320	Hericium erinaceus	303:320	Hericium erinaceus (H. erinaceus)	303:335	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	62	theme	H.	323:324	arg1	erinaceus					326:334	H. erinaceus	323:334	H. erinaceus	323:334	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	6	63	theme	kinases	1238:1244	arg1	phosphorylation					1131:1145	phosphorylation	1131:1145	phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt)	1131:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	3	64	theme	-induced	602:609	arg1	colitis					611:617	dextran sulfate sodium (DSS)-induced colitis	574:617	dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice	574:633	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	1	65	theme	bowel	215:219	arg1	disease					221:227	Inflammatory bowel disease	202:227	Inflammatory bowel disease (IBD)	202:233	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	1	65	theme	bowel	215:219	arg1	disease					240:246	a disease	238:246	a disease caused by a dysregulated immune with unknown etiology	238:300	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	1	65	theme	bowel	215:219	arg1	IBD					230:232	IBD	230:232	IBD	230:232	Inflammatory bowel disease (IBD) is a disease caused by a dysregulated immune with unknown etiology.
29677563	3	66	dep	HECP	537:540	arg1	Mw					543:544	Mw	543:544	Mw	543:544	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	3	66	dep	HECP	537:540	arg1	kDa					553:555	86.67 kDa	547:555	86.67 kDa	547:555	In this study, we have tested the anti-inflammatory effect of polysaccharide of H. erinaceus (HECP, Mw: 86.67 kDa) in the model of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
29677563	7	67	theme	intestinal	1366:1375	arg1	integrity					1385:1393	intestinal barrier integrity	1366:1393	intestinal barrier integrity	1366:1393	Moreover, HECP reversed DSS-induced gut dysbiosis and maintained intestinal barrier integrity.
29677563	4	68	theme	stresses	740:747	arg1	markers					719:725	key markers	715:725	key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO)	715:862	Our data indicated that HECP could improve clinical symptoms and down-regulate key markers of oxidative stresses, including nitric oxide (NO), malondialdehyde (MDA), total superoxide dismutase (T-SOD), and myeloperoxidase (MPO).
29677563	8	69	from	injury	1451:1456	arg1	mice					1461:1464	mice	1461:1464	mice	1461:1464	In conclusion, HECP ameliorates DSS-induced intestinal injury in mice, which suggests that HECP can serve as a protective dietary nutrient against IBD.
29677563	2	70	theme	medicinal	350:358	arg1	erinaceus					312:320	Hericium erinaceus	303:320	Hericium erinaceus (H. erinaceus)	303:335	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	2	70	theme	medicinal	350:358	arg1	fungus					360:365	a Chinese medicinal fungus	340:365	a Chinese medicinal fungus	340:365	Hericium erinaceus (H. erinaceus) is a Chinese medicinal fungus, with the effect of prevention and treatment of gastrointestinal disorders.
29677563	6	71	theme	B	1272:1272	arg1	phosphorylation					1131:1145	phosphorylation	1131:1145	phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt)	1131:1278	Meanwhile, HECP blocked phosphorylation of nuclear factor-κB (NF-κB) p65, NF-κB inhibitor alpha (IκB-α), mitogen-activated protein kinases (MAPK) and Protein kinase B (Akt) in DSS-treated mice.
29677563	7	72	theme	barrier	1377:1383	arg1	integrity					1385:1393	intestinal barrier integrity	1366:1393	intestinal barrier integrity	1366:1393	Moreover, HECP reversed DSS-induced gut dysbiosis and maintained intestinal barrier integrity.
31393892	8	0	theme	bacteria	1311:1318	arg1	abundance					1287:1295	the relative abundance	1274:1295	the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1274:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	1	1	theme	gastrointestinal	260:275	arg1	tract					277:281	the equine gastrointestinal tract	249:281	the equine gastrointestinal tract	249:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	12	2	theme	bacterial	1832:1840	arg1	community					1842:1850	the bacterial community	1828:1850	the bacterial community in the foregut (especially the stomach)	1828:1890	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	11	3	theme	gastrointestinal	1777:1792	arg1	tract					1794:1798	the entire gastrointestinal tract	1766:1798	the entire gastrointestinal tract	1766:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	3	4	theme	bwt	654:656	arg1	quantity					632:639	a quantity	630:639	a quantity of 0.15 g/kg bwt x d-1	630:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	3	5	theme	oat	484:486	arg1	grains					488:493	1.5 kg hay/100 kg bwt x d-1 and oat grains	452:493	1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	452:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	1	6	theme	study	122:126	arg1	objective					104:112	The objective	100:112	The objective of this study	100:126	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	3	7	theme	0.15	644:647	arg1	bwt					654:656	0.15 g/kg bwt	644:656	0.15 g/kg bwt	644:656	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	3	8	theme	d-1	476:478	arg1	grains					488:493	1.5 kg hay/100 kg bwt x d-1 and oat grains	452:493	1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	452:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	1	9	theme	active	179:184	arg1	compounds					186:194	natural prebiotic active compounds	161:194	natural prebiotic active compounds	161:194	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	10	10	theme	tract	1647:1651	arg1	parts					1617:1621	nearly all parts	1606:1621	nearly all parts of the gastrointestinal tract (P < 0.05)	1606:1662	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	2	11	theme	[bwt	317:320	arg1	horses					297:302	Twelve adult horses	284:302	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years)	284:356	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	2	11	theme	[bwt	317:320	arg1	kg					334:335	body weight [bwt] 534 ± 64.5 kg	305:335	body weight [bwt] 534 ± 64.5 kg	305:335	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	6	12	theme	gastrointestinal	852:867	arg1	tract					869:873	the gastrointestinal tract	848:873	the gastrointestinal tract	848:873	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	10	13	theme	P	1654:1654	arg1	tract					1647:1651	the gastrointestinal tract	1626:1651	the gastrointestinal tract (P < 0.05)	1626:1662	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	10	13	theme	P	1654:1654	arg1	<					1656:1656	P < 0.05	1654:1661	P < 0.05	1654:1661	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	3	14	theme	d-1	660:662	arg1	quantity					632:639	a quantity	630:639	a quantity of 0.15 g/kg bwt x d-1	630:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	3	15	theme	equal	495:499	arg1	grains					488:493	1.5 kg hay/100 kg bwt x d-1 and oat grains	452:493	1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	452:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	1	16	theme	microbial	203:211	arg1	composition					213:223	the microbial composition	199:223	the microbial composition in different regions of the equine gastrointestinal tract	199:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	8	17	dep	families	1366:1373	arg1	Ruminococcaceae					1406:1420	Ruminococcaceae	1406:1420	Ruminococcaceae	1406:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	17	dep	families	1366:1373	arg1	families					1366:1373	the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1362:1420	the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1362:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	17	dep	families	1366:1373	arg1	Lachnospiraceae					1375:1389	Lachnospiraceae	1375:1389	Lachnospiraceae (P < 0.05)	1375:1400	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	17	dep	families	1366:1373	arg1	<					1394:1394	P < 0.05	1392:1399	P < 0.05	1392:1399	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	3	18	theme	x	525:525	arg1	d-1					527:529	1.19 g starch/kg bwt x d-1	504:529	1.19 g starch/kg bwt x d-1	504:529	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	1	19	from	composition	213:223	arg1	regions					238:244	different regions	228:244	different regions of the equine gastrointestinal tract	228:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	6	20	theme	different	829:837	arg1	parts					839:843	different parts	829:843	different parts of the gastrointestinal tract	829:873	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	7	21	from	reduction	1069:1077	arg1	stomach					1136:1142	the stomach	1132:1142	the stomach (P < 0.05)	1132:1153	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	7	21	from	reduction	1069:1077	arg1	<					1147:1147	P < 0.05	1145:1152	P < 0.05	1145:1152	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	3	22	theme	starch/kg	511:519	arg1	d-1					527:529	1.19 g starch/kg bwt x d-1	504:529	1.19 g starch/kg bwt x d-1	504:529	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	12	23	from	pronounced	1901:1910	arg1	comparison					1915:1924	comparison	1915:1924	comparison to the effect in the hindgut	1915:1953	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	5	24	theme	period	800:805	arg1	end					781:783	the end	777:783	the end of the feeding period	777:805	The horses were fed for 21 d and euthanized at the end of the feeding period.
31393892	2	25	dep	kg	334:335	arg1	years					351:355	age 14 ± 7.5 years	338:355	age 14 ± 7.5 years	338:355	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	8	26	theme	unclassified	1338:1349	arg1	bacteria					1311:1318	fibrolytic bacteria	1300:1318	fibrolytic bacteria	1300:1318	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	26	theme	unclassified	1338:1349	arg1	members					1351:1357	the unclassified members	1334:1357	the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1334:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	7	27	theme	abundance	1095:1103	arg1	reduction					1069:1077	a concurrent reduction	1056:1077	a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05)	1056:1153	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	1	28	theme	natural	161:167	arg1	compounds					186:194	natural prebiotic active compounds	161:194	natural prebiotic active compounds	161:194	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	8	29	theme	families	1366:1373	arg1	bacteria					1311:1318	fibrolytic bacteria	1300:1318	fibrolytic bacteria	1300:1318	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	29	theme	families	1366:1373	arg1	members					1351:1357	the unclassified members	1334:1357	the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1334:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	30	theme	supplemental	1176:1187	arg1	prebiotic					1189:1197	the supplemental prebiotic	1172:1197	the supplemental prebiotic	1172:1197	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	11	31	theme	entire	1770:1775	arg1	tract					1794:1798	the entire gastrointestinal tract	1766:1798	the entire gastrointestinal tract	1766:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	10	32	theme	all	1613:1615	arg1	parts					1617:1621	nearly all parts	1606:1621	nearly all parts of the gastrointestinal tract (P < 0.05)	1606:1662	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	0	33	theme	meal	78:81	arg1	supplementation					83:97	meal supplementation	78:97	meal supplementation	78:97	Modification of the equine gastrointestinal microbiota by Jerusalem artichoke meal supplementation.
31393892	12	34	from	community	1842:1850	arg1	foregut					1859:1865	the foregut	1855:1865	the foregut (especially the stomach)	1855:1890	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	9	35	theme	relative	1427:1434	arg1	abundance					1436:1444	The relative abundance	1423:1444	The relative abundance of the genus Ruminococcus	1423:1470	The relative abundance of the genus Ruminococcus increased solely in the caecum and colon transversum.
31393892	6	36	theme	rRNA	933:936	arg1	gene					938:941	the 16S rRNA gene	925:941	the 16S rRNA gene	925:941	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	13	37	from	impact	1971:1976	arg1	health					1989:1994	stomach health	1981:1994	stomach health	1981:1994	Therefore, the impact on stomach health should be carefully considered.
31393892	4	38	theme	remaining	669:677	arg1	horses					679:684	The remaining horses	665:684	The remaining horses	665:684	The remaining horses received a placebo added to the basal diet.
31393892	3	39	theme	x	474:474	arg1	d-1					476:478	1.5 kg hay/100 kg bwt x d-1	452:478	1.5 kg hay/100 kg bwt x d-1	452:478	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	8	40	theme	Lactobacillus	1240:1252	arg1	abundance					1227:1235	the relative abundance	1214:1235	the relative abundance of Lactobacillus	1214:1252	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	0	41	theme	gastrointestinal	27:42	arg1	microbiota					44:53	the equine gastrointestinal microbiota	16:53	the equine gastrointestinal microbiota	16:53	Modification of the equine gastrointestinal microbiota by Jerusalem artichoke meal supplementation.
31393892	11	42	contain	had	1722:1724	arg2	impact					1729:1734	an impact	1726:1734	an impact on the microbial community in the entire gastrointestinal tract	1726:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	11	42	contain	had	1722:1724	arg1	feeding					1669:1675	The feeding	1665:1675	The feeding of this natural prebiotic compound to horses	1665:1720	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	7	43	theme	P	1040:1040	arg1	Lactobacillus					1025:1037	Lactobacillus	1025:1037	Lactobacillus (P < 0.05)	1025:1048	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	7	43	theme	P	1040:1040	arg1	<					1042:1042	P < 0.05	1040:1047	P < 0.05	1040:1047	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	9	44	theme	genus	1453:1457	arg1	Ruminococcus					1459:1470	the genus Ruminococcus	1449:1470	the genus Ruminococcus	1449:1470	The relative abundance of the genus Ruminococcus increased solely in the caecum and colon transversum.
31393892	3	45	theme	artichoke	560:568	arg1	meal					570:573	Jerusalem artichoke meal	550:573	Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	550:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	11	46	theme	prebiotic	1693:1701	arg1	compound					1703:1710	this natural prebiotic compound	1680:1710	this natural prebiotic compound	1680:1710	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	7	47	theme	Lactobacillus	1025:1037	arg1	abundance					1012:1020	the relative abundance	999:1020	the relative abundance of Lactobacillus (P < 0.05)	999:1048	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	7	48	theme	P	1145:1145	arg1	stomach					1136:1142	the stomach	1132:1142	the stomach (P < 0.05)	1132:1153	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	7	48	theme	P	1145:1145	arg1	<					1147:1147	P < 0.05	1145:1152	P < 0.05	1145:1152	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	6	49	from	parts	839:843	arg1	samples					816:822	Digesta samples	808:822	Digesta samples from different parts of the gastrointestinal tract	808:873	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	2	50	theme	±	327:327	arg1	horses					297:302	Twelve adult horses	284:302	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years)	284:356	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	2	50	theme	±	327:327	arg1	kg					334:335	body weight [bwt] 534 ± 64.5 kg	305:335	body weight [bwt] 534 ± 64.5 kg	305:335	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	1	51	theme	different	228:236	arg1	regions					238:244	different regions	228:244	different regions of the equine gastrointestinal tract	228:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	4	52	theme	basal	718:722	arg1	diet					724:727	the basal diet	714:727	the basal diet	714:727	The remaining horses received a placebo added to the basal diet.
31393892	3	53	theme	kg	467:468	arg1	d-1					476:478	1.5 kg hay/100 kg bwt x d-1	452:478	1.5 kg hay/100 kg bwt x d-1	452:478	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	6	54	theme	Digesta	808:814	arg1	samples					816:822	Digesta samples	808:822	Digesta samples from different parts of the gastrointestinal tract	808:873	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	7	55	theme	relative	1003:1010	arg1	abundance					1012:1020	the relative abundance	999:1020	the relative abundance of Lactobacillus (P < 0.05)	999:1048	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	2	56	theme	age	338:340	arg1	years					351:355	age 14 ± 7.5 years	338:355	age 14 ± 7.5 years	338:355	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	1	57	theme	equine	253:258	arg1	tract					277:281	the equine gastrointestinal tract	249:281	the equine gastrointestinal tract	249:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	9	58	theme	colon	1507:1511	arg1	transversum					1513:1523	colon transversum	1507:1523	colon transversum	1507:1523	The relative abundance of the genus Ruminococcus increased solely in the caecum and colon transversum.
31393892	7	59	theme	Streptococcus	1108:1120	arg1	abundance					1095:1103	the relative abundance	1082:1103	the relative abundance of Streptococcus	1082:1120	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	1	60	theme	tract	277:281	arg1	regions					238:244	different regions	228:244	different regions of the equine gastrointestinal tract	228:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	2	61	theme	adult	291:295	arg1	horses					297:302	Twelve adult horses	284:302	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years)	284:356	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	2	61	theme	adult	291:295	arg1	kg					334:335	body weight [bwt] 534 ± 64.5 kg	305:335	body weight [bwt] 534 ± 64.5 kg	305:335	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	1	62	theme	prebiotic	169:177	arg1	compounds					186:194	natural prebiotic active compounds	161:194	natural prebiotic active compounds	161:194	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	3	63	theme	g/kg	649:652	arg1	bwt					654:656	0.15 g/kg bwt	644:656	0.15 g/kg bwt	644:656	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	1	64	theme	compounds	186:194	arg1	impact					151:156	the impact	147:156	the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract	147:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	6	65	theme	tract	869:873	arg1	parts					839:843	different parts	829:843	different parts of the gastrointestinal tract	829:873	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	2	66	theme	weight	310:315	arg1	horses					297:302	Twelve adult horses	284:302	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years)	284:356	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	2	66	theme	weight	310:315	arg1	kg					334:335	body weight [bwt] 534 ± 64.5 kg	305:335	body weight [bwt] 534 ± 64.5 kg	305:335	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	3	67	theme	1.19	504:507	arg1	d-1					527:529	1.19 g starch/kg bwt x d-1	504:529	1.19 g starch/kg bwt x d-1	504:529	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	2	68	theme	±	345:345	arg1	years					351:355	age 14 ± 7.5 years	338:355	age 14 ± 7.5 years	338:355	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	8	69	theme	fibrolytic	1300:1309	arg1	bacteria					1311:1318	fibrolytic bacteria	1300:1318	fibrolytic bacteria	1300:1318	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	69	theme	fibrolytic	1300:1309	arg1	members					1351:1357	the unclassified members	1334:1357	the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1334:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	9	70	dep	caecum	1496:1501	arg1	the					1492:1494	the	1492:1494	the	1492:1494	The relative abundance of the genus Ruminococcus increased solely in the caecum and colon transversum.
31393892	3	71	theme	kg	456:457	arg1	d-1					476:478	1.5 kg hay/100 kg bwt x d-1	452:478	1.5 kg hay/100 kg bwt x d-1	452:478	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	10	72	theme	prebiotic	1555:1563	arg1	addition					1539:1546	the addition	1535:1546	the addition of the prebiotic	1535:1563	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	3	73	theme	bwt	521:523	arg1	d-1					527:529	1.19 g starch/kg bwt x d-1	504:529	1.19 g starch/kg bwt x d-1	504:529	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	11	74	from	community	1753:1761	arg1	tract					1794:1798	the entire gastrointestinal tract	1766:1798	the entire gastrointestinal tract	1766:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	3	75	theme	g	509:509	arg1	d-1					527:529	1.19 g starch/kg bwt x d-1	504:529	1.19 g starch/kg bwt x d-1	504:529	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	12	76	dep	foregut	1859:1865	arg1	stomach					1883:1889	the stomach	1879:1889	especially the stomach	1868:1889	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	5	77	theme	feeding	792:798	arg1	period					800:805	the feeding period	788:805	the feeding period	788:805	The horses were fed for 21 d and euthanized at the end of the feeding period.
31393892	10	78	from	diversity	1593:1601	arg1	parts					1617:1621	nearly all parts	1606:1621	nearly all parts of the gastrointestinal tract (P < 0.05)	1606:1662	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	10	79	theme	gastrointestinal	1630:1645	arg1	tract					1647:1651	the gastrointestinal tract	1626:1651	the gastrointestinal tract (P < 0.05)	1626:1662	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	10	79	theme	gastrointestinal	1630:1645	arg1	<					1656:1656	P < 0.05	1654:1661	P < 0.05	1654:1661	Overall, the addition of the prebiotic significantly increased the diversity in nearly all parts of the gastrointestinal tract (P < 0.05).
31393892	6	80	theme	V1-V2	909:913	arg1	region					915:920	the V1-V2 region	905:920	the V1-V2 region of the 16S rRNA gene	905:941	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	11	81	from	impact	1729:1734	arg1	community					1753:1761	the microbial community	1739:1761	the microbial community in the entire gastrointestinal tract	1739:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	7	82	theme	relative	1086:1093	arg1	abundance					1095:1103	the relative abundance	1082:1103	the relative abundance of Streptococcus	1082:1120	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	13	83	theme	stomach	1981:1987	arg1	health					1989:1994	stomach health	1981:1994	stomach health	1981:1994	Therefore, the impact on stomach health should be carefully considered.
31393892	7	84	theme	concurrent	1058:1067	arg1	reduction					1069:1077	a concurrent reduction	1056:1077	a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05)	1056:1153	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	2	85	theme	body	305:308	arg1	horses					297:302	Twelve adult horses	284:302	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years)	284:356	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	2	85	theme	body	305:308	arg1	kg					334:335	body weight [bwt] 534 ± 64.5 kg	305:335	body weight [bwt] 534 ± 64.5 kg	305:335	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	8	86	theme	P	1392:1392	arg1	Lachnospiraceae					1375:1389	Lachnospiraceae	1375:1389	Lachnospiraceae (P < 0.05)	1375:1400	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	8	86	theme	P	1392:1392	arg1	<					1394:1394	P < 0.05	1392:1399	P < 0.05	1392:1399	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	1	87	from	impact	151:156	arg1	composition					213:223	the microbial composition	199:223	the microbial composition in different regions of the equine gastrointestinal tract	199:281	The objective of this study was to investigate the impact of natural prebiotic active compounds on the microbial composition in different regions of the equine gastrointestinal tract.
31393892	11	88	theme	microbial	1743:1751	arg1	community					1753:1761	the microbial community	1739:1761	the microbial community in the entire gastrointestinal tract	1739:1798	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	12	89	from	effect	1933:1938	arg1	hindgut					1947:1953	the hindgut	1943:1953	the hindgut	1943:1953	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	6	90	theme	gene	938:941	arg1	region					915:920	the V1-V2 region	905:920	the V1-V2 region of the 16S rRNA gene	905:941	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	0	91	theme	equine	20:25	arg1	microbiota					44:53	the equine gastrointestinal microbiota	16:53	the equine gastrointestinal microbiota	16:53	Modification of the equine gastrointestinal microbiota by Jerusalem artichoke meal supplementation.
31393892	6	92	theme	16S	929:931	arg1	gene					938:941	the 16S rRNA gene	925:941	the 16S rRNA gene	925:941	Digesta samples from different parts of the gastrointestinal tract were taken, DNA extracted and the V1-V2 region of the 16S rRNA gene amplified.
31393892	2	93	theme	feeding	389:395	arg1	groups					397:402	two feeding groups	385:402	two feeding groups	385:402	Twelve adult horses (body weight [bwt] 534 ± 64.5 kg; age 14 ± 7.5 years) were randomly divided into two feeding groups.
31393892	8	94	theme	relative	1218:1225	arg1	abundance					1227:1235	the relative abundance	1214:1235	the relative abundance of Lactobacillus	1214:1252	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	3	95	theme	basal	427:431	arg1	diet					433:436	a basal diet	425:436	a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	425:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	0	96	theme	microbiota	44:53	arg1	Modification					0:11	Modification	0:11	Modification of the equine gastrointestinal microbiota by Jerusalem	0:66	Modification of the equine gastrointestinal microbiota by Jerusalem artichoke meal supplementation.
31393892	12	97	from	effect	1818:1823	arg1	community					1842:1850	the bacterial community	1828:1850	the bacterial community in the foregut (especially the stomach)	1828:1890	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	3	98	theme	Jerusalem	550:558	arg1	meal					570:573	Jerusalem artichoke meal	550:573	Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1	550:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	9	99	theme	Ruminococcus	1459:1470	arg1	abundance					1436:1444	The relative abundance	1423:1444	The relative abundance of the genus Ruminococcus	1423:1470	The relative abundance of the genus Ruminococcus increased solely in the caecum and colon transversum.
31393892	7	100	with	Supplementation	954:968	arg1	prebiotic					979:987	the prebiotic	975:987	the prebiotic	975:987	Supplementation with the prebiotic increased the relative abundance of Lactobacillus (P < 0.05), with a concurrent reduction of the relative abundance of Streptococcus mainly in the stomach (P < 0.05).
31393892	3	101	theme	hay/100	459:465	arg1	d-1					476:478	1.5 kg hay/100 kg bwt x d-1	452:478	1.5 kg hay/100 kg bwt x d-1	452:478	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	3	102	theme	1.5	452:454	arg1	kg					456:457	kg	456:457	kg	456:457	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	11	103	theme	compound	1703:1710	arg1	feeding					1669:1675	The feeding	1665:1675	The feeding of this natural prebiotic compound to horses	1665:1720	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	3	104	theme	prebiotic	585:593	arg1	fructooligosaccharides					595:616	prebiotic fructooligosaccharides	585:616	prebiotic fructooligosaccharides	585:616	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	12	105	from	comparison	1915:1924	arg1	pronounced					1901:1910	pronounced	1901:1910	pronounced	1901:1910	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	12	105	from	comparison	1915:1924	arg1	effect					1818:1823	the effect	1814:1823	the effect on the bacterial community in the foregut (especially the stomach)	1814:1890	Furthermore, the effect on the bacterial community in the foregut (especially the stomach) was more pronounced in comparison to the effect in the hindgut.
31393892	11	106	theme	natural	1685:1691	arg1	compound					1703:1710	this natural prebiotic compound	1680:1710	this natural prebiotic compound	1680:1710	The feeding of this natural prebiotic compound to horses had an impact on the microbial community in the entire gastrointestinal tract.
31393892	3	107	from	fructooligosaccharides	595:616	arg1	quantity					632:639	a quantity	630:639	a quantity of 0.15 g/kg bwt x d-1	630:662	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31393892	8	108	theme	relative	1278:1285	arg1	abundance					1287:1295	the relative abundance	1274:1295	the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae	1274:1420	In the hindgut, the supplemental prebiotic also increased the relative abundance of Lactobacillus but further reduced the relative abundance of fibrolytic bacteria, specifically the unclassified members of the families Lachnospiraceae (P < 0.05) and Ruminococcaceae.
31393892	3	109	theme	bwt	470:472	arg1	d-1					476:478	1.5 kg hay/100 kg bwt x d-1	452:478	1.5 kg hay/100 kg bwt x d-1	452:478	Six horses received a basal diet consisting of 1.5 kg hay/100 kg bwt x d-1 and oat grains equal to 1.19 g starch/kg bwt x d-1, supplemented with Jerusalem artichoke meal providing prebiotic fructooligosaccharides + inulin in a quantity of 0.15 g/kg bwt x d-1.
31260767	0	0	theme	essential	89:97	arg1	oils					99:102	natural essential oils	81:102	natural essential oils	81:102	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	1	1	theme	starch	295:300	arg1	polymers					302:309	starch polymers	295:309	starch polymers (0.75:1.5:0.75 w/w)	295:329	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	1	1	theme	starch	295:300	arg1	w/w					326:328	0.75:1.5:0.75 w/w	312:328	0.75:1.5:0.75 w/w	312:328	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	5	2	theme	REO	724:726	arg1	Incorporation					707:719	Incorporation	707:719	Incorporation of REO and nisin	707:736	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	7	3	from	incorporation	1128:1140	arg1	films					1186:1190	active biocomposite films	1166:1190	active biocomposite films	1166:1190	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	1	4	theme	mint	146:149	arg1	MEO					166:168	MEO	166:168	MEO	166:168	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	1	4	theme	mint	146:149	arg1	oil					161:163	mint essential oil	146:163	mint essential oil (MEO)	146:169	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	6	5	theme	metal	970:974	arg1	chelation					976:984	metal chelation	970:984	metal chelation	970:984	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	6	theme	natural	81:87	arg1	oils					99:102	natural essential oils	81:102	natural essential oils	81:102	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	2	7	theme	tensile	438:444	arg1	strength					446:453	tensile strength	438:453	tensile strength (25.95 MPa)	438:465	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	2	7	theme	tensile	438:444	arg1	25.95 MPa					456:464	25.95 MPa	456:464	25.95 MPa	456:464	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	1	8	theme	essential	151:159	arg1	MEO					166:168	MEO	166:168	MEO	166:168	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	1	8	theme	essential	151:159	arg1	oil					161:163	mint essential oil	146:163	mint essential oil (MEO)	146:169	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	2	9	theme	thermal	471:477	arg1	stability					479:487	thermal stability	471:487	thermal stability	471:487	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	4	10	dep	increased	646:654	arg1	2.78					700:703	2.78	700:703	2.78	700:703	Addition of REO of and nisin increased microsctructural heterogeneity and opacity (2.78).
31260767	6	11	dep	enhanced	1034:1041	arg1	p ≤ 0.05					1058:1065	p ≤ 0.05	1058:1065	p ≤ 0.05	1058:1065	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	6	12	theme	power	963:967	arg1	assays					1020:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	13	theme	oils	99:102	arg1	Incorporation					64:76	Incorporation	64:76	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.	0:128	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	7	14	theme	functional	1209:1218	arg1	ingredients					1220:1230	promising functional ingredients	1199:1230	promising functional ingredients for packaging materials for various food applications	1199:1284	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	7	15	theme	promising	1199:1207	arg1	ingredients					1220:1230	promising functional ingredients	1199:1230	promising functional ingredients for packaging materials for various food applications	1199:1284	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	6	16	theme	reducing	954:961	arg1	power					963:967	reducing power	954:967	reducing power	954:967	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	17	theme	agents	122:127	arg1	Incorporation					64:76	Incorporation	64:76	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.	0:128	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	6	18	theme	scavenging	1009:1018	arg1	assays					1020:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	19	theme	antimicrobial	108:120	arg1	agents					122:127	antimicrobial agents	108:127	antimicrobial agents	108:127	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	5	20	theme	Listeria	860:867	arg1	monocytogenes					869:881	Listeria monocytogenes	860:881	Listeria monocytogenes	860:881	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	5	21	theme	nisin	732:736	arg1	Incorporation					707:719	Incorporation	707:719	Incorporation of REO and nisin	707:736	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	2	22	theme	water	393:397	arg1	0.014 g.mm/m224h					419:434	0.014 g.mm/m224h	419:434	0.014 g.mm/m224h	419:434	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	2	22	theme	water	393:397	arg1	properties					407:416	water barrier properties	393:416	water barrier properties (0.014 g.mm/m224h)	393:435	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	2	23	theme	REO	345:347	arg1	Inclusion					332:340	Inclusion	332:340	Inclusion of REO and nisin	332:357	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	2	24	theme	barrier	399:405	arg1	0.014 g.mm/m224h					419:434	0.014 g.mm/m224h	419:434	0.014 g.mm/m224h	419:434	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	2	24	theme	barrier	399:405	arg1	properties					407:416	water barrier properties	393:416	water barrier properties (0.014 g.mm/m224h)	393:435	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	6	25	theme	ABTS	996:999	arg1	scavenging					1009:1018	ABTS radical scavenging	996:1018	ABTS radical scavenging	996:1018	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	7	26	theme	biocomposite	1173:1184	arg1	films					1186:1190	active biocomposite films	1166:1190	active biocomposite films	1166:1190	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	3	27	theme	ATR-FTIR	513:520	arg1	spectra					522:528	ATR-FTIR spectra	513:528	ATR-FTIR spectra	513:528	ATR-FTIR spectra and XRD pattern elucidated structural interaction between EO's and biocomposite films.
31260767	4	28	theme	REO	629:631	arg1	Addition					617:624	Addition	617:624	Addition of REO of and nisin	617:644	Addition of REO of and nisin increased microsctructural heterogeneity and opacity (2.78).
31260767	0	29	theme	Functional	0:9	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.	0:128	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	6	30	theme	films	928:932	arg1	properties					901:910	The antioxidant properties	885:910	The antioxidant properties of biocomposite films	885:932	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	31	theme	biopolymer	31:40	arg1	film					58:61	biopolymer based composite film	31:61	biopolymer based composite film	31:61	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	1	32	theme	novel	233:237	arg1	film					260:263	a novel functional packaging film	231:263	a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w)	231:329	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	6	33	theme	REO	1097:1099	arg1	addition					1077:1084	the addition	1073:1084	the addition of MEO and REO	1073:1099	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	5	34	theme	tested	792:797	arg1	strains					810:816	all tested pathogenic strains	788:816	all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes)	788:882	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	1	35	theme	functional	239:248	arg1	film					260:263	a novel functional packaging film	231:263	a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w)	231:329	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	3	36	theme	biocomposite	597:608	arg1	films					610:614	biocomposite films	597:614	biocomposite films	597:614	ATR-FTIR spectra and XRD pattern elucidated structural interaction between EO's and biocomposite films.
31260767	4	37	theme	nisin	640:644	arg1	REO					629:631	REO	629:631	REO of and nisin	629:644	Addition of REO of and nisin increased microsctructural heterogeneity and opacity (2.78).
31260767	5	38	theme	pathogenic	799:808	arg1	strains					810:816	all tested pathogenic strains	788:816	all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes)	788:882	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	0	39	theme	composite	48:56	arg1	film					58:61	biopolymer based composite film	31:61	biopolymer based composite film	31:61	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	1	40	theme	packaging	250:258	arg1	film					260:263	a novel functional packaging film	231:263	a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w)	231:329	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	7	41	theme	food	1268:1271	arg1	applications					1273:1284	various food applications	1260:1284	various food applications	1260:1284	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	2	42	dep	significantly	359:371	arg1	p ≤ 0.05					374:381	p ≤ 0.05	374:381	p ≤ 0.05	374:381	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	6	43	theme	radical	1001:1007	arg1	scavenging					1009:1018	ABTS radical scavenging	996:1018	ABTS radical scavenging	996:1018	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	44	theme	based	42:46	arg1	film					58:61	biopolymer based composite film	31:61	biopolymer based composite film	31:61	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	6	45	theme	biocomposite	915:926	arg1	films					928:932	biocomposite films	915:932	biocomposite films	915:932	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	1	46	theme	lactic	182:187	arg1	acid					189:192	lactic acid	182:192	lactic acid	182:192	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	7	47	theme	additives	1153:1161	arg1	incorporation					1128:1140	incorporation	1128:1140	incorporation of natural additives in active biocomposite films	1128:1190	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	5	48	theme	highest	752:758	arg1	activity					771:778	the highest inhibitory activity	748:778	the highest inhibitory activity	748:778	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	6	49	theme	MEO	1089:1091	arg1	addition					1077:1084	the addition	1073:1084	the addition of MEO and REO	1073:1099	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	0	50	theme	film	58:61	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.	0:128	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	1	51	theme	chitosan	275:282	arg1	pectin					284:289	chitosan pectin	275:289	chitosan pectin	275:289	Rosemary (REO), mint essential oil (MEO), nisin and lactic acid were incorporated at 0.5% to develop a novel functional packaging film including chitosan pectin and starch polymers (0.75:1.5:0.75 w/w).
31260767	7	52	theme	natural	1145:1151	arg1	additives					1153:1161	natural additives	1145:1161	natural additives	1145:1161	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	3	53	theme	XRD	534:536	arg1	pattern					538:544	XRD pattern	534:544	XRD pattern	534:544	ATR-FTIR spectra and XRD pattern elucidated structural interaction between EO's and biocomposite films.
31260767	4	54	theme	microsctructural	656:671	arg1	heterogeneity					673:685	microsctructural heterogeneity	656:685	microsctructural heterogeneity	656:685	Addition of REO of and nisin increased microsctructural heterogeneity and opacity (2.78).
31260767	6	55	theme	antioxidant	889:899	arg1	properties					901:910	The antioxidant properties	885:910	The antioxidant properties of biocomposite films	885:932	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	5	56	dep	strains	810:816	arg1	subtilis					828:835	Bacillus subtilis	819:835	Bacillus subtilis	819:835	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	5	56	dep	strains	810:816	arg1	coli					850:853	Escherichia coli	838:853	Escherichia coli	838:853	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	5	56	dep	strains	810:816	arg1	monocytogenes					869:881	Listeria monocytogenes	860:881	Listeria monocytogenes	860:881	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	7	57	theme	packaging	1236:1244	arg1	materials					1246:1254	packaging materials	1236:1254	packaging materials	1236:1254	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	3	58	theme	structural	557:566	arg1	interaction					568:578	structural interaction	557:578	structural interaction between EO's and biocomposite films	557:614	ATR-FTIR spectra and XRD pattern elucidated structural interaction between EO's and biocomposite films.
31260767	7	59	theme	active	1166:1171	arg1	films					1186:1190	active biocomposite films	1166:1190	active biocomposite films	1166:1190	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	6	60	theme	DPPH	987:990	arg1	assays					1020:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31260767	2	61	theme	nisin	353:357	arg1	Inclusion					332:340	Inclusion	332:340	Inclusion of REO and nisin	332:357	Inclusion of REO and nisin significantly (p ≤ 0.05) improved water barrier properties (0.014 g.mm/m224h), tensile strength (25.95 MPa) and thermal stability as compared to control.
31260767	0	62	dep	characterization	11:26	arg1	Incorporation					64:76	Incorporation	64:76	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.	0:128	Functional characterization of biopolymer based composite film: Incorporation of natural essential oils and antimicrobial agents.
31260767	7	63	theme	various	1260:1266	arg1	applications					1273:1284	various food applications	1260:1284	various food applications	1260:1284	This study validated that incorporation of natural additives in active biocomposite films offers promising functional ingredients for packaging materials for various food applications.
31260767	5	64	theme	inhibitory	760:769	arg1	activity					771:778	the highest inhibitory activity	748:778	the highest inhibitory activity	748:778	Incorporation of REO and nisin exhibited the highest inhibitory activity against all tested pathogenic strains (Bacillus subtilis, Escherichia coli, and Listeria monocytogenes).
31260767	6	65	theme	chelation	976:984	arg1	assays					1020:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	reducing power, metal chelation, DPPH and ABTS radical scavenging assays	954:1025	The antioxidant properties of biocomposite films were analyzed using reducing power, metal chelation, DPPH and ABTS radical scavenging assays, which enhanced significantly (p ≤ 0.05) with the addition of MEO and REO.
31027001	0	0	theme	Low-Temperature	75:89	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	1	1	theme	electrolyte	161:171	arg1	membrane					173:180	A successful polymer electrolyte membrane	140:180	A successful polymer electrolyte membrane for fuel cell application	140:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	1	2	theme	good	259:262	arg1	capability					280:289	good water retention capability	259:289	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	2	3	theme	CS	543:544	arg1	characteristics					524:538	the water uptake characteristics	507:538	the water uptake characteristics of CS	507:544	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	3	theme	CS	543:544	arg1	hydrophilicity					488:501	hydrophilicity	488:501	hydrophilicity	488:501	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	4	theme	deacetylated	360:371	arg1	chitosan					373:380	85% deacetylated chitosan	356:380	85% deacetylated chitosan (CS)	356:385	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	4	theme	deacetylated	360:371	arg1	CS					383:384	CS	383:384	CS	383:384	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	1	5	theme	water	264:268	arg1	capability					280:289	good water retention capability	259:289	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	0	6	theme	Exchange	98:105	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	7	7	theme	membranes	1163:1171	arg1	cost-effectiveness					1103:1120	cost-effectiveness	1103:1120	cost-effectiveness	1103:1120	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	7	7	theme	membranes	1163:1171	arg1	fabrication					1133:1143	simple fabrication	1126:1143	simple fabrication	1126:1143	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	4	8	theme	membranes	806:814	arg1	interactions					786:797	the interactions	782:797	the interactions of the membranes with water	782:825	Absorption studies were carried out to evaluate the interactions of the membranes with water.
31027001	1	9	theme	retention	270:278	arg1	capability					280:289	good water retention capability	259:289	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	0	10	theme	Proton	91:96	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	6	11	theme	proton	1031:1036	arg1	conductivity					1038:1049	its relatively high proton conductivity	1011:1049	its relatively high proton conductivity	1011:1049	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	0	12	theme	Fuel	116:119	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	7	13	theme	such	1148:1151	arg1	membranes					1163:1171	such composite membranes	1148:1171	such composite membranes	1148:1171	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	5	14	theme	capacity	853:860	arg1	analysis					862:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	0	15	theme	Membrane	107:114	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	6	16	theme	CS/Nafion®	935:944	arg1	blend					946:950	The CS/Nafion® blend	931:950	The CS/Nafion® blend	931:950	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	6	16	theme	CS/Nafion®	935:944	arg1	suitable					968:975	suitable	968:975	suitable	968:975	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	7	17	theme	composite	1153:1161	arg1	membranes					1163:1171	such composite membranes	1148:1171	such composite membranes	1148:1171	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	7	18	theme	simple	1126:1131	arg1	fabrication					1133:1143	simple fabrication	1126:1143	simple fabrication	1126:1143	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	1	19	theme	fuel	186:189	arg1	application					196:206	fuel cell application	186:206	fuel cell application	186:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	0	20	theme	High	10:13	arg1	Conductivity					22:33	High Proton Conductivity	10:33	High Proton Conductivity	10:33	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	7	21	theme	economical	1234:1243	arg1	PEMFCs					1207:1212	low-temperature PEMFCs	1191:1212	low-temperature PEMFCs very attractive and economical	1191:1243	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	1	22	theme	cell	191:194	arg1	application					196:206	fuel cell application	186:206	fuel cell application	186:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	6	23	theme	high	1026:1029	arg1	conductivity					1038:1049	its relatively high proton conductivity	1011:1049	its relatively high proton conductivity	1011:1049	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	3	24	theme	intermolecular	676:689	arg1	interactions					691:702	their intermolecular interactions	670:702	their intermolecular interactions	670:702	These membranes were characterized by infrared spectroscopy and field-emission scanning electron microscopy to investigate their intermolecular interactions and morphology, respectively.
31027001	5	25	theme	active	901:906	arg1	sites					908:912	active sites	901:912	active sites in the membrane	901:928	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	2	26	theme	composite	315:323	arg1	membranes					325:333	composite membranes	315:333	composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs)	315:445	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	27	theme	fuel	427:430	arg1	PEMFCs					439:444	PEMFCs	439:444	PEMFCs	439:444	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	27	theme	fuel	427:430	arg1	cells					432:436	proton exchange membrane fuel cells	402:436	proton exchange membrane fuel cells (PEMFCs)	402:445	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	0	28	theme	Proton	15:20	arg1	Conductivity					22:33	High Proton Conductivity	10:33	High Proton Conductivity	10:33	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	0	29	theme	Cell	121:124	arg1	Applications					126:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Low-Temperature Proton Exchange Membrane Fuel Cell Applications	75:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	4	30	theme	Absorption	734:743	arg1	studies					745:751	Absorption studies	734:751	Absorption studies	734:751	Absorption studies were carried out to evaluate the interactions of the membranes with water.
31027001	2	31	theme	membrane	418:425	arg1	fuel					427:430	proton exchange membrane fuel	402:430	proton exchange membrane fuel cells (PEMFCs)	402:445	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	4	32	with	interactions	786:797	arg1	water					821:825	water	821:825	water	821:825	Absorption studies were carried out to evaluate the interactions of the membranes with water.
31027001	2	33	theme	exchange	409:416	arg1	fuel					427:430	proton exchange membrane fuel	402:430	proton exchange membrane fuel cells (PEMFCs)	402:445	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	1	34	theme	efficient	218:226	arg1	conductivity					235:246	efficient proton conductivity	218:246	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	5	35	theme	Titrimetric	828:838	arg1	analysis					862:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	2	36	theme	characteristics	524:538	arg1	concept					477:483	the concept	473:483	the concept of hydrophilicity and the water uptake characteristics of CS	473:544	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	7	37	theme	attractive	1219:1228	arg1	PEMFCs					1207:1212	low-temperature PEMFCs	1191:1212	low-temperature PEMFCs very attractive and economical	1191:1243	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	5	38	theme	sites	908:912	arg1	availability					885:896	the availability	881:896	the availability of active sites in the membrane	881:928	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	1	39	theme	proton	228:233	arg1	conductivity					235:246	efficient proton conductivity	218:246	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	5	40	theme	ion	840:842	arg1	analysis					862:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	2	41	theme	uptake	517:522	arg1	characteristics					524:538	the water uptake characteristics	507:538	the water uptake characteristics of CS	507:544	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	7	42	dep	cost-effectiveness	1103:1120	arg1	the					1099:1101	the	1099:1101	the	1099:1101	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	5	43	from	sites	908:912	arg1	membrane					921:928	the membrane	917:928	the membrane	917:928	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	2	44	theme	%	358:358	arg1	chitosan					373:380	85% deacetylated chitosan	356:380	85% deacetylated chitosan (CS)	356:385	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	44	theme	%	358:358	arg1	CS					383:384	CS	383:384	CS	383:384	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	5	45	theme	exchange	844:851	arg1	analysis					862:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis	828:869	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	6	46	theme	PEMFC	981:985	arg1	applications					987:998	PEMFC applications	981:998	PEMFC applications	981:998	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	3	47	theme	infrared	585:592	arg1	spectroscopy					594:605	infrared spectroscopy	585:605	infrared spectroscopy	585:605	These membranes were characterized by infrared spectroscopy and field-emission scanning electron microscopy to investigate their intermolecular interactions and morphology, respectively.
31027001	5	48	from	availability	885:896	arg1	membrane					921:928	the membrane	917:928	the membrane	917:928	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	2	49	theme	85	356:357	arg1	%					358:358	%	358:358	%	358:358	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	7	50	from	use	1184:1186	arg1	PEMFCs					1207:1212	low-temperature PEMFCs	1191:1212	low-temperature PEMFCs very attractive and economical	1191:1243	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	7	51	theme	low-temperature	1191:1205	arg1	PEMFCs					1207:1212	low-temperature PEMFCs	1191:1212	low-temperature PEMFCs very attractive and economical	1191:1243	Above all, the cost-effectiveness and simple fabrication of such composite membranes make their use in low-temperature PEMFCs very attractive and economical.
31027001	3	52	theme	electron	635:642	arg1	microscopy					644:653	field-emission scanning electron microscopy	611:653	field-emission scanning electron microscopy	611:653	These membranes were characterized by infrared spectroscopy and field-emission scanning electron microscopy to investigate their intermolecular interactions and morphology, respectively.
31027001	1	53	contain	have	213:216	arg2	capability					280:289	good water retention capability	259:289	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	1	53	contain	have	213:216	arg2	conductivity					235:246	efficient proton conductivity	218:246	efficient proton conductivity as well as good water retention capability	218:289	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	1	53	contain	have	213:216	arg1	membrane					173:180	A successful polymer electrolyte membrane	140:180	A successful polymer electrolyte membrane for fuel cell application	140:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	5	54	from	membrane	921:928	arg1	availability					885:896	the availability	881:896	the availability of active sites in the membrane	881:928	Titrimetric ion exchange capacity analysis indicated the availability of active sites in the membrane.
31027001	6	55	theme	regular	1071:1077	arg1	Nafion®					1079:1085	regular Nafion®	1071:1085	regular Nafion®	1071:1085	The CS/Nafion® blend was found to be suitable for PEMFC applications because of its relatively high proton conductivity compared to that of regular Nafion®.
31027001	2	56	theme	hydrophilicity	488:501	arg1	concept					477:483	the concept	473:483	the concept of hydrophilicity and the water uptake characteristics of CS	473:544	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	2	57	theme	proton	402:407	arg1	fuel					427:430	proton exchange membrane fuel	402:430	proton exchange membrane fuel cells (PEMFCs)	402:445	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
31027001	3	58	theme	field-emission	611:624	arg1	microscopy					644:653	field-emission scanning electron microscopy	611:653	field-emission scanning electron microscopy	611:653	These membranes were characterized by infrared spectroscopy and field-emission scanning electron microscopy to investigate their intermolecular interactions and morphology, respectively.
31027001	1	59	theme	successful	142:151	arg1	membrane					173:180	A successful polymer electrolyte membrane	140:180	A successful polymer electrolyte membrane for fuel cell application	140:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	0	60	theme	Acidic	55:60	arg1	Chitosan					62:69	Acidic Chitosan	55:69	Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications	55:137	Imparting High Proton Conductivity to Nafion® Tuned by Acidic Chitosan for Low-Temperature Proton Exchange Membrane Fuel Cell Applications.
31027001	3	61	theme	scanning	626:633	arg1	microscopy					644:653	field-emission scanning electron microscopy	611:653	field-emission scanning electron microscopy	611:653	These membranes were characterized by infrared spectroscopy and field-emission scanning electron microscopy to investigate their intermolecular interactions and morphology, respectively.
31027001	1	62	theme	polymer	153:159	arg1	membrane					173:180	A successful polymer electrolyte membrane	140:180	A successful polymer electrolyte membrane for fuel cell application	140:206	A successful polymer electrolyte membrane for fuel cell application must have efficient proton conductivity as well as good water retention capability.
31027001	2	63	theme	water	511:515	arg1	characteristics					524:538	the water uptake characteristics	507:538	the water uptake characteristics of CS	507:544	The viability of using composite membranes prepared by blending 85% deacetylated chitosan (CS) and Nafion® in proton exchange membrane fuel cells (PEMFCs) was investigated based on the concept of hydrophilicity and the water uptake characteristics of CS.
30892147	6	0	theme	removed	1267:1273	arg1	fractions					1275:1283	the removed fractions	1263:1283	the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+)	1263:1338	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	4	1	theme	K	844:844	arg1	rate					838:841	the diffusion rate	824:841	the diffusion rate (K d)	824:847	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	4	1	theme	K	844:844	arg1	d					846:846	K d	844:846	K d	844:846	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	2	2	theme	Fourier-transform	352:368	arg1	spectroscopy					379:390	Fourier-transform infrared spectroscopy	352:390	Fourier-transform infrared spectroscopy	352:390	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	6	3	theme	ion	1236:1238	arg1	Pb2+					1240:1243	the post-transition metal ion Pb2+	1210:1243	the post-transition metal ion Pb2+	1210:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	4	theme	solution	1031:1038	arg1	treatment					1001:1009	The treatment	997:1009	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode	997:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	1	5	theme	hydrogel	265:272	arg1	beads					299:303	STNTs-Ch beads	290:303	STNTs-Ch beads	290:303	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	1	5	theme	hydrogel	265:272	arg1	beads					283:287	hydrogel chitosan beads	265:287	hydrogel chitosan beads (STNTs-Ch beads)	265:304	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	0	6	theme	hydrogel	103:110	arg1	beads					121:125	hydrogel chitosan beads	103:125	hydrogel chitosan beads	103:125	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate nanotubes embedded in hydrogel chitosan beads.
30892147	5	7	theme	2.3	954:956	arg1	times					958:962	times	958:962	times	958:962	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	5	7	theme	2.3	954:956	arg1	capacity					909:916	the maximum capacity	897:916	the maximum capacity of adsorption of STNTs-Ch beads	897:948	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	5	8	theme	adsorption	921:930	arg1	capacity					909:916	the maximum capacity	897:916	the maximum capacity of adsorption of STNTs-Ch beads	897:948	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	5	8	theme	adsorption	921:930	arg1	times					958:962	times	958:962	times	958:962	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	1	9	theme	chitosan	274:281	arg1	beads					299:303	STNTs-Ch beads	290:303	STNTs-Ch beads	290:303	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	1	9	theme	chitosan	274:281	arg1	beads					283:287	hydrogel chitosan beads	265:287	hydrogel chitosan beads (STNTs-Ch beads)	265:304	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	8	10	theme	wastewater	1584:1593	arg1	treatment					1595:1603	wastewater treatment	1584:1603	wastewater treatment	1584:1603	This composite was produced in a simple way and shows the potential for wastewater treatment.
30892147	8	11	theme	simple	1545:1550	arg1	way					1552:1554	a simple way	1543:1554	a simple way	1543:1554	This composite was produced in a simple way and shows the potential for wastewater treatment.
30892147	6	12	theme	Cd2+	1026:1029	arg1	solution					1031:1038	a synthetic Cd2+ solution	1014:1038	a synthetic Cd2+ solution	1014:1038	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	4	13	theme	kinetic	779:785	arg1	k					793:793	k 2	793:795	k 2	793:795	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	4	13	theme	kinetic	779:785	arg1	rate					787:790	the kinetic rate	775:790	the kinetic rate (k 2)	775:796	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	2	14	theme	scanning	410:417	arg1	microscopy					428:437	scanning electron microscopy	410:437	scanning electron microscopy	410:437	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	0	15	theme	chitosan	112:119	arg1	beads					121:125	hydrogel chitosan beads	103:125	hydrogel chitosan beads	103:125	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate nanotubes embedded in hydrogel chitosan beads.
30892147	5	16	theme	beads	944:948	arg1	adsorption					921:930	adsorption	921:930	adsorption of STNTs-Ch beads	921:948	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	1	17	theme	mesoporous	177:186	arg1	composite					188:196	a nanoparticle-based mesoporous composite	156:196	a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption	156:340	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	7	18	theme	saving	1411:1416	arg1	times					1424:1428	saving three times	1411:1428	saving three times the needed amounts of TiO2	1411:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	2	19	theme	X-ray	471:475	arg1	spectrometer					477:488	an energy-dispersive X-ray spectrometer	450:488	an energy-dispersive X-ray spectrometer	450:488	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	4	20	theme	diffusion	828:836	arg1	rate					838:841	the diffusion rate	824:841	the diffusion rate (K d)	824:847	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	4	20	theme	diffusion	828:836	arg1	d					846:846	K d	844:846	K d	844:846	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	6	21	from	treatment	1001:1009	arg1	mode					1074:1077	continuous mode	1063:1077	continuous mode	1063:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	4	22	theme	adsorption	736:745	arg1	studies					747:753	The adsorption studies	732:753	The adsorption studies of Cd2+	732:761	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	2	23	theme	energy-dispersive	453:469	arg1	spectrometer					477:488	an energy-dispersive X-ray spectrometer	450:488	an energy-dispersive X-ray spectrometer	450:488	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	1	24	theme	STNTs-Ch	290:297	arg1	beads					299:303	STNTs-Ch beads	290:303	STNTs-Ch beads	290:303	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	1	24	theme	STNTs-Ch	290:297	arg1	beads					283:287	hydrogel chitosan beads	265:287	hydrogel chitosan beads (STNTs-Ch beads)	265:304	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	6	25	theme	Pb2+	1240:1243	arg1	fractions					1137:1145	higher removed fractions	1122:1145	higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+	1122:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	4	26	theme	Cd2+	758:761	arg1	studies					747:753	The adsorption studies	732:753	The adsorption studies of Cd2+	732:761	The adsorption studies of Cd2+ showed that the kinetic rate (k 2) increased 3-fold and that the diffusion rate (K d) increased 2-fold after the embedment.
30892147	5	27	dep	times	958:962	arg1	higher					964:969	higher	964:969	higher	964:969	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	0	28	theme	cadmium	14:20	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate	0:79	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate nanotubes embedded in hydrogel chitosan beads.
30892147	5	29	theme	maximum	901:907	arg1	capacity					909:916	the maximum capacity	897:916	the maximum capacity of adsorption of STNTs-Ch beads	897:948	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	5	29	theme	maximum	901:907	arg1	times					958:962	times	958:962	times	958:962	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	0	30	from	embedded	91:98	arg1	beads					121:125	hydrogel chitosan beads	103:125	hydrogel chitosan beads	103:125	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate nanotubes embedded in hydrogel chitosan beads.
30892147	6	31	dep	ions	1167:1170	arg1	Cd2+					1173:1176	Cd2+	1173:1176	Cd2+	1173:1176	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	31	dep	ions	1167:1170	arg1	Co2+					1179:1182	Co2+	1179:1182	Co2+	1179:1182	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	31	dep	ions	1167:1170	arg1	Cu2+					1200:1203	Cu2+	1200:1203	Cu2+	1200:1203	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	31	dep	ions	1167:1170	arg1	ions					1167:1170	transition metal ions	1150:1170	transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+)	1150:1204	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	31	dep	ions	1167:1170	arg1	Ni2+					1185:1188	Ni2+	1185:1188	Ni2+	1185:1188	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	31	dep	ions	1167:1170	arg1	Zn2+					1191:1194	Zn2+	1191:1194	Zn2+	1191:1194	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	32	theme	metal	1312:1316	arg1	ions					1318:1321	alkali and alkali-earth metal ions	1288:1321	ions	1318:1321	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	33	theme	metal	1230:1234	arg1	Pb2+					1240:1243	the post-transition metal ion Pb2+	1210:1243	the post-transition metal ion Pb2+	1210:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	7	34	theme	material	1502:1509	arg1	production					1484:1493	the production	1480:1493	the production of the material	1480:1509	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	1	35	theme	silicate-titanate	216:232	arg1	STNTs					245:249	STNTs	245:249	STNTs	245:249	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	1	35	theme	silicate-titanate	216:232	arg1	nanotubes					234:242	silicate-titanate nanotubes	216:242	silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads)	216:304	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	1	36	theme	nanoparticle-based	158:175	arg1	composite					188:196	a nanoparticle-based mesoporous composite	156:196	a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption	156:340	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	6	37	located	observed	1113:1120	arg1	phases					1090:1095	two phases	1086:1095	two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+)	1086:1338	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	37	located	observed	1113:1120	arg1	comparison					1249:1258	comparison	1249:1258	comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+)	1249:1338	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	37	located	observed	1113:1120	arg2	it					1106:1107	it	1106:1107	it	1106:1107	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	38	theme	ions	1167:1170	arg1	fractions					1137:1145	higher removed fractions	1122:1145	higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+	1122:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	2	39	theme	electron	419:426	arg1	microscopy					428:437	scanning electron microscopy	410:437	scanning electron microscopy	410:437	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	6	40	theme	metal	1161:1165	arg1	Cd2+					1173:1176	Cd2+	1173:1176	Cd2+	1173:1176	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	40	theme	metal	1161:1165	arg1	Co2+					1179:1182	Co2+	1179:1182	Co2+	1179:1182	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	40	theme	metal	1161:1165	arg1	Cu2+					1200:1203	Cu2+	1200:1203	Cu2+	1200:1203	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	40	theme	metal	1161:1165	arg1	ions					1167:1170	transition metal ions	1150:1170	transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+)	1150:1204	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	40	theme	metal	1161:1165	arg1	Ni2+					1185:1188	Ni2+	1185:1188	Ni2+	1185:1188	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	40	theme	metal	1161:1165	arg1	Zn2+					1191:1194	Zn2+	1191:1194	Zn2+	1191:1194	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	41	from	leachate	1051:1058	arg1	mode					1074:1077	continuous mode	1063:1077	continuous mode	1063:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	42	dep	removed	1129:1135	arg1	higher					1122:1127	higher	1122:1127	higher	1122:1127	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	43	theme	alkali	1288:1293	arg1	ions					1318:1321	alkali and alkali-earth metal ions	1288:1321	ions	1318:1321	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	44	dep	ions	1318:1321	arg1	Mg2+					1334:1337	Mg2+	1334:1337	Mg2+	1334:1337	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	44	dep	ions	1318:1321	arg1	K+					1330:1331	K+	1330:1331	K+	1330:1331	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	44	dep	ions	1318:1321	arg1	Ca2+					1324:1327	Ca2+	1324:1327	Ca2+	1324:1327	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	0	45	from	beads	121:125	arg1	embedded					91:98	embedded	91:98	embedded	91:98	Adsorption of cadmium by a high-capacity adsorbent composed of silicate-titanate nanotubes embedded in hydrogel chitosan beads.
30892147	6	46	theme	transition	1150:1159	arg1	Cd2+					1173:1176	Cd2+	1173:1176	Cd2+	1173:1176	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	46	theme	transition	1150:1159	arg1	Co2+					1179:1182	Co2+	1179:1182	Co2+	1179:1182	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	46	theme	transition	1150:1159	arg1	Cu2+					1200:1203	Cu2+	1200:1203	Cu2+	1200:1203	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	46	theme	transition	1150:1159	arg1	ions					1167:1170	transition metal ions	1150:1170	transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+)	1150:1204	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	46	theme	transition	1150:1159	arg1	Ni2+					1185:1188	Ni2+	1185:1188	Ni2+	1185:1188	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	46	theme	transition	1150:1159	arg1	Zn2+					1191:1194	Zn2+	1191:1194	Zn2+	1191:1194	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	47	from	mode	1074:1077	arg1	treatment					1001:1009	The treatment	997:1009	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode	997:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	48	theme	continuous	1063:1072	arg1	mode					1074:1077	continuous mode	1063:1077	continuous mode	1063:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	49	theme	alkali-earth	1299:1310	arg1	ions					1318:1321	alkali and alkali-earth metal ions	1288:1321	ions	1318:1321	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	2	50	theme	beads	581:585	arg1	walls					561:565	the walls	557:565	the walls of the hollow beads	557:585	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	6	51	theme	post-transition	1214:1228	arg1	Pb2+					1240:1243	the post-transition metal ion Pb2+	1210:1243	the post-transition metal ion Pb2+	1210:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	2	52	theme	hollow	574:579	arg1	beads					581:585	the hollow beads	570:585	the hollow beads	570:585	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	7	53	dep	times	1424:1428	arg1	amounts					1441:1447	the needed amounts	1430:1447	saving three times the needed amounts of TiO2	1411:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	7	53	dep	times	1424:1428	arg1	TiO2					1452:1455	TiO2	1452:1455	TiO2	1452:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	3	54	theme	pH	635:636	arg1	effect					625:630	the effect	621:630	the effect of pH	621:636	The dispersion was attributed to the effect of pH when the composite was prepared and we observed a non-interaction between STNTs and chitosan.
30892147	6	55	theme	leachate	1051:1058	arg1	treatment					1001:1009	The treatment	997:1009	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode	997:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	7	56	theme	TiO2	1452:1455	arg1	amounts					1441:1447	the needed amounts	1430:1447	saving three times the needed amounts of TiO2	1411:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	7	56	theme	TiO2	1452:1455	arg1	TiO2					1452:1455	TiO2	1452:1455	TiO2	1452:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	5	57	theme	STNTs-Ch	935:942	arg1	beads					944:948	STNTs-Ch beads	935:948	STNTs-Ch beads	935:948	Moreover, the maximum capacity of adsorption of STNTs-Ch beads was 2.3 times higher than that of STNTs alone.
30892147	6	58	theme	removed	1129:1135	arg1	fractions					1137:1145	higher removed fractions	1122:1145	higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+	1122:1243	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	59	from	solution	1031:1038	arg1	mode					1074:1077	continuous mode	1063:1077	continuous mode	1063:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	60	theme	real	1046:1049	arg1	leachate					1051:1058	a real leachate	1044:1058	a real leachate in continuous mode	1044:1077	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	6	61	theme	synthetic	1016:1024	arg1	solution					1031:1038	a synthetic Cd2+ solution	1014:1038	a synthetic Cd2+ solution	1014:1038	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	7	62	theme	needed	1434:1439	arg1	amounts					1441:1447	the needed amounts	1430:1447	saving three times the needed amounts of TiO2	1411:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	7	62	theme	needed	1434:1439	arg1	TiO2					1452:1455	TiO2	1452:1455	TiO2	1452:1455	The composite was successfully reused four times when adsorbing Cd2+, saving three times the needed amounts of TiO2, SiO2 and chitosan for the production of the material.
30892147	2	63	theme	hollow	519:524	arg1	STNTs					526:530	the hollow STNTs	515:530	the hollow STNTs	515:530	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
30892147	1	64	theme	Cd2+	326:329	arg1	adsorption					331:340	Cd2+ adsorption	326:340	Cd2+ adsorption	326:340	In this study, we developed a nanoparticle-based mesoporous composite that consisted of silicate-titanate nanotubes (STNTs) supported in hydrogel chitosan beads (STNTs-Ch beads) and was studied for Cd2+ adsorption.
30892147	6	65	theme	ions	1318:1321	arg1	fractions					1275:1283	the removed fractions	1263:1283	the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+)	1263:1338	The treatment of a synthetic Cd2+ solution and a real leachate in continuous mode showed two phases in which it was observed higher removed fractions of transition metal ions (Cd2+, Co2+, Ni2+, Zn2+ and Cu2+) and the post-transition metal ion Pb2+, in comparison to the removed fractions of alkali and alkali-earth metal ions (Ca2+, K+, Mg2+).
30892147	2	66	theme	infrared	370:377	arg1	spectroscopy					379:390	Fourier-transform infrared spectroscopy	352:390	Fourier-transform infrared spectroscopy	352:390	By using Fourier-transform infrared spectroscopy, transmission and scanning electron microscopy coupled to an energy-dispersive X-ray spectrometer, we could determine that the hollow STNTs were highly dispersed in the walls of the hollow beads.
31728643	9	0	theme	fibrous	1247:1253	arg1	mesh					1255:1258	The fibrous mesh	1243:1258	The fibrous mesh	1243:1258	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	10	1	theme	suitable	1450:1457	arg1	stability					1459:1467	a suitable stability	1448:1467	a suitable stability in aqueous environment	1448:1490	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	4	2	theme	biomimetic	699:708	arg1	patch					710:714	a suturable biomimetic patch	687:714	a suturable biomimetic patch	687:714	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	12	3	theme	alginate/gelatin	1800:1815	arg1	sponges					1817:1823	the alginate/gelatin sponges	1796:1823	the alginate/gelatin sponges	1796:1823	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	9	4	theme	material	1352:1359	arg1	hydrophilicity					1330:1343	hydrophilicity	1330:1343	hydrophilicity of the material	1330:1359	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	11	5	theme	Polycaprolactone	1599:1614	arg1	mesh					1616:1619	Polycaprolactone mesh	1599:1619	Polycaprolactone mesh	1599:1619	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	6	6	from	place	1039:1043	arg1	center					1077:1082	the center	1073:1082	the center of the sponge	1073:1096	Reinforced scaffolds were obtained through the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge.
31728643	4	7	theme	suturable	689:697	arg1	patch					710:714	a suturable biomimetic patch	687:714	a suturable biomimetic patch	687:714	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	11	8	dep	cardiomyocytes	1687:1700	arg1	cardiomyocytes					1687:1700	cardiomyocytes adhesion and proliferation	1687:1727	cardiomyocytes adhesion and proliferation	1687:1727	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	11	8	dep	cardiomyocytes	1687:1700	arg1	proliferation					1715:1727	proliferation	1715:1727	proliferation	1715:1727	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	11	8	dep	cardiomyocytes	1687:1700	arg1	adhesion					1702:1709	adhesion	1702:1709	adhesion	1702:1709	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	12	9	theme	potential	1928:1936	arg1	use					1938:1940	their potential use	1922:1940	their potential use in myocardial tissue engineering	1922:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	5	10	theme	microfibers	884:894	arg1	layers					857:862	eight superimposed layers	838:862	eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one	838:952	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	4	11	theme	synthetic	738:746	arg1	mesh					748:751	a synthetic mesh	736:751	a synthetic mesh	736:751	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	2	12	theme	biomimetic	310:319	arg1	sponges					321:327	new biomimetic sponges	306:327	new biomimetic sponges	306:327	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	6	13	theme	sponge	1091:1096	arg1	center					1077:1082	the center	1073:1082	the center of the sponge	1073:1096	Reinforced scaffolds were obtained through the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge.
31728643	12	14	theme	reinforcement	1865:1877	arg1	system					1879:1884	an appropriate reinforcement system	1850:1884	an appropriate reinforcement system	1850:1884	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	3	15	theme	cardiac	529:535	arg1	matrix					551:556	a promising cardiac extracellular matrix	517:556	a promising cardiac extracellular matrix substitute	517:567	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	3	15	theme	cardiac	529:535	arg1	scaffolds					479:487	these scaffolds	473:487	these scaffolds	473:487	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	6	16	theme	fibrous	1049:1055	arg1	mesh					1057:1060	the fibrous mesh	1045:1060	the fibrous mesh	1045:1060	Reinforced scaffolds were obtained through the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge.
31728643	12	17	theme	obtained	1743:1750	arg1	results					1752:1758	the obtained results	1739:1758	the obtained results	1739:1758	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	8	18	theme	perfect	1177:1183	arg1	integration					1185:1195	A perfect integration	1175:1195	A perfect integration between the mesh and the sponge	1175:1227	A perfect integration between the mesh and the sponge was observed.
31728643	3	19	theme	extracellular	537:549	arg1	matrix					551:556	a promising cardiac extracellular matrix	517:556	a promising cardiac extracellular matrix substitute	517:567	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	3	19	theme	extracellular	537:549	arg1	scaffolds					479:487	these scaffolds	473:487	these scaffolds	473:487	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	4	20	theme	work	659:662	arg1	Aim					640:642	Aim	640:642	Aim of the present work	640:662	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	4	20	theme	work	659:662	arg1	development					672:682	the development	668:682	the development of a suturable biomimetic patch	668:714	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	10	21	theme	reinforced	1549:1558	arg1	scaffold					1560:1567	not reinforced scaffold	1545:1567	not reinforced scaffold	1545:1567	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	10	22	from	stability	1459:1467	arg1	environment					1480:1490	aqueous environment	1472:1490	aqueous environment	1472:1490	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	9	23	theme	sponge	1288:1293	arg1	capacity					1272:1279	the capacity	1268:1279	the capacity of the sponge to absorb water	1268:1309	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	1	24	theme	3D	91:92	arg1	scaffolds					94:102	3D scaffolds	91:102	3D scaffolds used to repair damaged tissues	91:133	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	7	25	theme	reinforced	1135:1144	arg1	scaffold					1146:1153	the reinforced scaffold	1131:1153	the reinforced scaffold	1131:1153	Both the reinforcement mesh and the reinforced scaffold were characterized.
31728643	6	26	theme	Reinforced	955:964	arg1	scaffolds					966:974	Reinforced scaffolds	955:974	Reinforced scaffolds	955:974	Reinforced scaffolds were obtained through the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge.
31728643	12	27	theme	system	1879:1884	arg1	insertion					1837:1845	the insertion	1833:1845	the insertion of an appropriate reinforcement system	1833:1884	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	2	28	theme	new	306:308	arg1	sponges					321:327	new biomimetic sponges	306:327	new biomimetic sponges	306:327	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	9	29	theme	natural	1395:1401	arg1	tissue					1411:1416	natural cardiac tissue	1395:1416	natural cardiac tissue	1395:1416	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	2	30	theme	engineering	426:436	arg1	applications					438:449	myocardial tissue engineering applications	408:449	myocardial tissue engineering applications	408:449	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	12	31	theme	myocardial	1945:1954	arg1	engineering					1963:1973	myocardial tissue engineering	1945:1973	myocardial tissue engineering	1945:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	4	32	theme	mesh	748:751	arg1	inclusion					723:731	the inclusion	719:731	the inclusion of a synthetic mesh within an alginate/gelatin scaffold	719:787	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	2	33	theme	myocardial	408:417	arg1	applications					438:449	myocardial tissue engineering applications	408:449	myocardial tissue engineering applications	408:449	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	2	34	theme	tissue	419:424	arg1	applications					438:449	myocardial tissue engineering applications	408:449	myocardial tissue engineering applications	408:449	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	11	35	theme	reinforced	1647:1656	arg1	able					1671:1674	able	1671:1674	able	1671:1674	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	11	35	theme	reinforced	1647:1656	arg1	scaffold					1658:1665	the reinforced scaffold	1643:1665	the reinforced scaffold	1643:1665	Polycaprolactone mesh was not cytotoxic and the reinforced scaffold was able to support cardiomyocytes adhesion and proliferation.
31728643	2	36	theme	previous	288:295	arg1	research					297:304	our previous research	284:304	our previous research	284:304	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	1	37	theme	natural	193:199	arg1	matrix					215:220	natural extracellular matrix	193:220	natural extracellular matrix	193:220	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	0	38	theme	suturable	38:46	arg1	patch					59:63	a suturable biomimetic patch	36:63	a suturable biomimetic patch for cardiac applications	36:88	Development and characterization of a suturable biomimetic patch for cardiac applications.
31728643	4	39	theme	patch	710:714	arg1	Aim					640:642	Aim	640:642	Aim of the present work	640:662	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	4	39	theme	patch	710:714	arg1	development					672:682	the development	668:682	the development of a suturable biomimetic patch	668:714	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	4	40	theme	present	651:657	arg1	work					659:662	the present work	647:662	the present work	647:662	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	10	41	theme	human	1583:1587	arg1	arteries					1589:1596	human arteries	1583:1596	human arteries	1583:1596	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	1	42	theme	extracellular	201:213	arg1	matrix					215:220	natural extracellular matrix	193:220	natural extracellular matrix	193:220	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	7	43	theme	reinforcement	1108:1120	arg1	mesh					1122:1125	the reinforcement mesh	1104:1125	the reinforcement mesh	1104:1125	Both the reinforcement mesh and the reinforced scaffold were characterized.
31728643	10	44	theme	reinforced	1423:1432	arg1	system					1434:1439	The reinforced system	1419:1439	The reinforced system	1419:1439	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	12	45	from	use	1938:1940	arg1	engineering					1963:1973	myocardial tissue engineering	1945:1973	myocardial tissue engineering	1945:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	5	46	theme	dry	812:814	arg1	spinning					816:823	dry spinning	812:823	dry spinning	812:823	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	1	47	theme	matrix	215:220	arg1	functions					180:188	functions	180:188	functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins	180:278	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	1	47	theme	matrix	215:220	arg1	composition					164:174	composition	164:174	composition	164:174	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	0	48	theme	patch	59:63	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of a suturable biomimetic patch for cardiac applications.
31728643	0	48	theme	patch	59:63	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of a suturable biomimetic patch for cardiac applications.
31728643	10	49	theme	aqueous	1472:1478	arg1	environment					1480:1490	aqueous environment	1472:1490	aqueous environment	1472:1490	The reinforced system showed a suitable stability in aqueous environment and it resulted much more resistant to suturing than not reinforced scaffold and even than human arteries.
31728643	9	50	with	comparable	1371:1380	arg1	that					1387:1390	that	1387:1390	that	1387:1390	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	1	51	theme	repair	112:117	arg1	tissues					127:133	repair damaged tissues	112:133	repair damaged tissues	112:133	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	0	52	theme	biomimetic	48:57	arg1	patch					59:63	a suturable biomimetic patch	36:63	a suturable biomimetic patch for cardiac applications	36:88	Development and characterization of a suturable biomimetic patch for cardiac applications.
31728643	12	53	theme	appropriate	1853:1863	arg1	system					1879:1884	an appropriate reinforcement system	1850:1884	an appropriate reinforcement system	1850:1884	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	1	54	theme	damaged	119:125	arg1	tissues					127:133	repair damaged tissues	112:133	repair damaged tissues	112:133	3D scaffolds used to repair damaged tissues should be able to mimic both composition and functions of natural extracellular matrix, which is mainly composed of polysaccharides and proteins.
31728643	12	55	theme	use	1938:1940	arg1	view					1914:1917	view	1914:1917	view of their potential use in myocardial tissue engineering	1914:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	2	56	with	blends	339:344	arg1	gelatin					363:369	gelatin	363:369	gelatin	363:369	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	3	57	dep	matrix	551:556	arg1	substitute					558:567	substitute	558:567	substitute	558:567	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	12	58	from	correct	1903:1909	arg1	view					1914:1917	view	1914:1917	view of their potential use in myocardial tissue engineering	1914:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	4	59	theme	alginate/gelatin	763:778	arg1	scaffold					780:787	an alginate/gelatin scaffold	760:787	an alginate/gelatin scaffold	760:787	Aim of the present work was the development of a suturable biomimetic patch by the inclusion of a synthetic mesh within an alginate/gelatin scaffold.
31728643	12	60	from	view	1914:1917	arg1	choice					1779:1784	the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system	1775:1884	the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system	1775:1884	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	12	60	from	view	1914:1917	arg1	engineering					1963:1973	myocardial tissue engineering	1945:1973	myocardial tissue engineering	1945:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	12	60	from	view	1914:1917	arg1	correct					1903:1909	correct	1903:1909	correct	1903:1909	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	5	61	theme	adjacent	941:948	arg1	one					950:952	the adjacent one	937:952	the adjacent one	937:952	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	2	62	theme	alginate	349:356	arg1	blends					339:344	blends	339:344	blends of alginate with gelatin	339:369	In our previous research new biomimetic sponges, based on blends of alginate with gelatin, were produced and characterized for myocardial tissue engineering applications.
31728643	9	63	theme	hydrophilicity	1330:1343	arg1	comparable					1371:1380	comparable	1371:1380	comparable	1371:1380	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	9	63	theme	hydrophilicity	1330:1343	arg1	degree					1320:1325	the degree	1316:1325	the degree of hydrophilicity of the material	1316:1359	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	0	64	theme	cardiac	69:75	arg1	applications					77:88	cardiac applications	69:88	cardiac applications	69:88	Development and characterization of a suturable biomimetic patch for cardiac applications.
31728643	6	65	theme	mold	1011:1014	arg1	use					1002:1004	the use	998:1004	the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge	998:1096	Reinforced scaffolds were obtained through the use of a mold, specially designed to place the fibrous mesh exactly in the center of the sponge.
31728643	12	66	from	engineering	1963:1973	arg1	view					1914:1917	view	1914:1917	view of their potential use in myocardial tissue engineering	1914:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31728643	9	67	theme	cardiac	1403:1409	arg1	tissue					1411:1416	natural cardiac tissue	1395:1416	natural cardiac tissue	1395:1416	The fibrous mesh reduced the capacity of the sponge to absorb water, but the degree of hydrophilicity of the material was still comparable with that of natural cardiac tissue.
31728643	5	68	theme	polycaprolactone	867:882	arg1	microfibers					884:894	polycaprolactone microfibers	867:894	polycaprolactone microfibers	867:894	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	5	68	theme	polycaprolactone	867:882	arg1	one					902:904	one	902:904	one	902:904	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	5	69	theme	superimposed	844:855	arg1	layers					857:862	eight superimposed layers	838:862	eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one	838:952	The mesh, produced by dry spinning, was made of eight superimposed layers of polycaprolactone microfibers, each one rotated of 45° with respect to the adjacent one.
31728643	3	70	theme	suturing	619:626	arg1	properties					628:637	their suturing properties	613:637	their suturing properties	613:637	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	3	71	theme	promising	519:527	arg1	matrix					551:556	a promising cardiac extracellular matrix	517:556	a promising cardiac extracellular matrix substitute	517:567	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	3	71	theme	promising	519:527	arg1	scaffolds					479:487	these scaffolds	473:487	these scaffolds	473:487	It was observed that these scaffolds can potentially function as a promising cardiac extracellular matrix substitute, but a reinforcement is required to improve their suturing properties.
31728643	12	72	theme	tissue	1956:1961	arg1	engineering					1963:1973	myocardial tissue engineering	1945:1973	myocardial tissue engineering	1945:1973	Overall, the obtained results confirmed that the choice to modify the alginate/gelatin sponges through the insertion of an appropriate reinforcement system turned out to be correct in view of their potential use in myocardial tissue engineering.
31454076	3	0	link	cross-linked	422:433	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	3	1	theme	ABGN-CSMA	507:515	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	1	2	theme	vessel	235:240	arg1	formation					242:250	blood vessel formation	229:250	blood vessel formation	229:250	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	7	3	theme	local	1033:1037	arg1	stability					1042:1050	local pH stability	1033:1050	local pH stability	1033:1050	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	5	4	theme	Surface	667:673	arg1	modification					685:696	Surface amination modification	667:696	Surface amination modification of BGNs	667:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	1	5	theme	formation	242:250	arg1	challenge					177:185	a critical challenge	166:185	a critical challenge of bone defect repair	166:207	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	1	5	theme	formation	242:250	arg1	promotion					216:224	the promotion	212:224	the promotion of blood vessel formation	212:250	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	5	6	theme	BGNs	742:745	arg1	dispersion					728:737	the dispersion	724:737	the dispersion of BGNs	724:745	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	5	7	theme	amination	675:683	arg1	modification					685:696	Surface amination modification	667:696	Surface amination modification of BGNs	667:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	3	8	theme	angiogenic	560:569	arg1	properties					571:580	angiogenic properties	560:580	angiogenic properties	560:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	4	9	theme	carboxyl	642:649	arg1	groups					651:656	the carboxyl groups	638:656	the carboxyl groups on CSMA	638:664	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	7	10	theme	hydrogel	1095:1102	arg1	stability					1042:1050	local pH stability	1033:1050	local pH stability	1033:1050	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	7	10	theme	hydrogel	1095:1102	arg1	release					1070:1076	sustained ion release	1056:1076	sustained ion release	1056:1076	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	2	11	theme	current	265:271	arg1	methods					273:279	the current methods	261:279	the current methods	261:279	Most of the current methods promote vascularization by adding specific growth factors, which are costly and easy to inactivate.
31454076	3	12	theme	sulfate	485:491	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	3	13	theme	glass	454:458	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	1	14	theme	Blood	91:95	arg1	vessels					97:103	Blood vessels	91:103	Blood vessels	91:103	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	4	15	theme	ABGNs	607:611	arg1	ABGNs					607:611	the ABGNs	603:611	the ABGNs	603:611	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	4	15	theme	ABGNs	607:611	arg1	groups					593:598	The amino groups	583:598	The amino groups of the ABGNs	583:611	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	6	16	theme	loss	891:894	arg1	modulus					896:902	the largest loss modulus	879:902	the largest loss modulus (560 Pa)	879:911	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	6	16	theme	loss	891:894	arg1	560 Pa					905:910	560 Pa	905:910	560 Pa	905:910	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	5	17	theme	mechanical	791:800	arg1	properties					802:811	the mechanical properties	787:811	the mechanical properties of the composite hydrogel	787:837	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	4	18	theme	covalent	618:625	arg1	bonds					627:631	covalent bonds	618:631	covalent bonds	618:631	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	1	19	theme	critical	168:175	arg1	challenge					177:185	a critical challenge	166:185	a critical challenge of bone defect repair	166:207	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	1	19	theme	critical	168:175	arg1	promotion					216:224	the promotion	212:224	the promotion of blood vessel formation	212:250	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	5	20	theme	composite	820:828	arg1	hydrogel					830:837	the composite hydrogel	816:837	the composite hydrogel	816:837	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	6	21	theme	largest	883:889	arg1	modulus					896:902	the largest loss modulus	879:902	the largest loss modulus (560 Pa)	879:911	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	6	21	theme	largest	883:889	arg1	560 Pa					905:910	560 Pa	905:910	560 Pa	905:910	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	7	22	theme	ion	1066:1068	arg1	release					1070:1076	sustained ion release	1056:1076	sustained ion release	1056:1076	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	5	23	theme	BGNs	701:704	arg1	modification					685:696	Surface amination modification	667:696	Surface amination modification of BGNs	667:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	8	24	theme	organic-inorganic	1244:1260	arg1	hydrogels					1272:1280	covalently cross-linked organic-inorganic composite hydrogels	1220:1280	covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties	1220:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	0	25	theme	Bioactive	0:8	arg1	glass					10:14	Bioactive glass	0:14	Bioactive glass	0:14	Bioactive glass functionalized chondroitin sulfate hydrogel with proangiogenic properties.
31454076	6	26	theme	%	987:987	arg1	concentration					989:1001	10% concentration	985:1001	10% concentration of ABGNs	985:1010	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	8	27	link	cross-linked	1231:1242	arg1	hydrogels					1272:1280	covalently cross-linked organic-inorganic composite hydrogels	1220:1280	covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties	1220:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	0	28	theme	chondroitin	31:41	arg1	hydrogel					51:58	chondroitin sulfate hydrogel	31:58	chondroitin sulfate hydrogel	31:58	Bioactive glass functionalized chondroitin sulfate hydrogel with proangiogenic properties.
31454076	5	29	theme	hydrogel	830:837	arg1	properties					802:811	the mechanical properties	787:811	the mechanical properties of the composite hydrogel	787:837	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	1	30	theme	important	113:121	arg1	role					123:126	an important role	110:126	an important role	110:126	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	1	31	theme	bone	190:193	arg1	repair					202:207	bone defect repair	190:207	bone defect repair	190:207	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	7	32	theme	sustained	1056:1064	arg1	release					1070:1076	sustained ion release	1056:1076	sustained ion release	1056:1076	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	5	33	mod	modification	685:696	arg1	BGNs					701:704	BGNs	701:704	BGNs	701:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	5	33	mod	modification	685:696	arg3	amination					675:683	Surface amination modification	667:696	Surface amination modification of BGNs	667:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	5	33	mod	modification	685:696	arg3	Surface					667:673	Surface amination modification	667:696	Surface amination modification of BGNs	667:704	Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel.
31454076	4	34	from	groups	651:656	arg1	CSMA					661:664	CSMA	661:664	CSMA	661:664	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	1	35	theme	defect	195:200	arg1	repair					202:207	bone defect repair	190:207	bone defect repair	190:207	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	3	36	with	hydrogel	546:553	arg1	properties					571:580	angiogenic properties	560:580	angiogenic properties	560:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	4	37	theme	amino	587:591	arg1	ABGNs					607:611	the ABGNs	603:611	the ABGNs	603:611	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	4	37	theme	amino	587:591	arg1	groups					593:598	The amino groups	583:598	The amino groups of the ABGNs	583:611	The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA.
31454076	7	38	theme	pH	1039:1040	arg1	stability					1042:1050	local pH stability	1033:1050	local pH stability	1033:1050	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	3	39	theme	cross-linked	422:433	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	8	40	theme	composite	1262:1270	arg1	hydrogels					1272:1280	covalently cross-linked organic-inorganic composite hydrogels	1220:1280	covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties	1220:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	8	41	theme	cross-linked	1231:1242	arg1	hydrogels					1272:1280	covalently cross-linked organic-inorganic composite hydrogels	1220:1280	covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties	1220:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	1	42	theme	repair	202:207	arg1	challenge					177:185	a critical challenge	166:185	a critical challenge of bone defect repair	166:207	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	1	42	theme	repair	202:207	arg1	promotion					216:224	the promotion	212:224	the promotion of blood vessel formation	212:250	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	0	43	theme	sulfate	43:49	arg1	hydrogel					51:58	chondroitin sulfate hydrogel	31:58	chondroitin sulfate hydrogel	31:58	Bioactive glass functionalized chondroitin sulfate hydrogel with proangiogenic properties.
31454076	6	44	theme	10	985:986	arg1	%					987:987	%	987:987	%	987:987	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	8	45	theme	angiogenic	1287:1296	arg1	properties					1298:1307	angiogenic properties	1287:1307	angiogenic properties	1287:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	3	46	theme	aminated	435:442	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	0	47	theme	proangiogenic	65:77	arg1	properties					79:88	proangiogenic properties	65:88	proangiogenic properties	65:88	Bioactive glass functionalized chondroitin sulfate hydrogel with proangiogenic properties.
31454076	8	48	theme	hydrogels	1272:1280	arg1	development					1205:1215	the development	1201:1215	the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties	1201:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	6	49	theme	hydrogel	964:971	arg1	deformation					945:955	deformation	945:955	deformation of the hydrogel	945:971	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	8	50	with	hydrogels	1272:1280	arg1	properties					1298:1307	angiogenic properties	1287:1307	angiogenic properties	1287:1307	This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
31454076	3	51	theme	methacrylate	493:504	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	7	52	theme	cell	1121:1124	arg1	adhesion					1126:1133	cell adhesion	1121:1133	cell adhesion	1121:1133	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	3	53	theme	nanoparticle-chondroitin	460:483	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	1	54	theme	bone	131:134	arg1	repair					143:148	bone defect repair	131:148	bone defect repair	131:148	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	6	55	theme	strongest	921:929	arg1	resistance					931:940	the strongest resistance	917:940	the strongest resistance to deformation of the hydrogel	917:971	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	6	56	theme	ABGNs	1006:1010	arg1	concentration					989:1001	10% concentration	985:1001	10% concentration of ABGNs	985:1010	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	1	57	theme	defect	136:141	arg1	repair					143:148	bone defect repair	131:148	bone defect repair	131:148	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
31454076	6	58	theme	storage	852:858	arg1	modulus					860:866	The largest storage modulus	840:866	The largest storage modulus (1200 Pa)	840:876	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	6	58	theme	storage	852:858	arg1	1200 Pa					869:875	1200 Pa	869:875	1200 Pa	869:875	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	7	59	theme	composite	1085:1093	arg1	hydrogel					1095:1102	the composite hydrogel	1081:1102	the composite hydrogel	1081:1102	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	2	60	theme	specific	315:322	arg1	factors					331:337	specific growth factors	315:337	specific growth factors	315:337	Most of the current methods promote vascularization by adding specific growth factors, which are costly and easy to inactivate.
31454076	2	61	theme	growth	324:329	arg1	factors					331:337	specific growth factors	315:337	specific growth factors	315:337	Most of the current methods promote vascularization by adding specific growth factors, which are costly and easy to inactivate.
31454076	6	62	theme	largest	844:850	arg1	modulus					860:866	The largest storage modulus	840:866	The largest storage modulus (1200 Pa)	840:876	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	6	62	theme	largest	844:850	arg1	1200 Pa					869:875	1200 Pa	869:875	1200 Pa	869:875	The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs.
31454076	3	63	theme	bioactive	444:452	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	3	64	theme	organic-inorganic	518:534	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	7	65	dep	stability	1042:1050	arg1	the					1029:1031	the	1029:1031	the	1029:1031	Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis.
31454076	3	66	theme	composite	536:544	arg1	hydrogel					546:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel	409:553	a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties	409:580	In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties.
31454076	1	67	theme	blood	229:233	arg1	formation					242:250	blood vessel formation	229:250	blood vessel formation	229:250	Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation.
30426288	0	0	theme	LPS-induced	98:108	arg1	over-activation					120:134	LPS-induced microglia over-activation	98:134	LPS-induced microglia over-activation	98:134	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	8	1	theme	activities	1104:1113	arg1	evaluation					1061:1070	The evaluation	1057:1070	The evaluation of their anti-neuroinflammatory activities	1057:1113	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	1	2	theme	cochinchinensis	186:200	arg1	resin					168:172	resin	168:172	resin of Dracaena cochinchinensis	168:200	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	1	2	theme	cochinchinensis	186:200	arg1	candidate					207:215	a candidate	205:215	a candidate for therapy of neurodegenerative diseases	205:257	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	7	3	theme	extract	905:911	arg1	separation					877:886	The bioassay-directed separation	855:886	The bioassay-directed separation of the effective extract of D. cochinchinensis	855:933	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	4	4	theme	microglial	555:564	arg1	cells					566:570	BV-2 microglial cells	550:570	BV-2 microglial cells stimulated with lipopolysaccharide (LPS)	550:611	The production of NO was determined using nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS).
30426288	2	5	theme	components	370:379	arg1	mechanism					343:351	the primary mechanism	331:351	the primary mechanism of the effective components	331:379	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	2	5	theme	components	370:379	arg1	basis					295:299	the material basis	282:299	the material basis of Chinese Dragon's blood	282:325	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	6	6	theme	mRNA	661:664	arg1	level					666:670	The mRNA level	657:670	The mRNA level of inducible nitric oxide synthase (iNOS)	657:712	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	11	7	theme	TNF-α	1782:1786	arg1	expressions					1752:1762	the expressions	1748:1762	the expressions of iNOS, IL-6 and TNF-α in over-activated microglial	1748:1815	These could inhibit neuroinflammation by reducing the expressions of iNOS, IL-6 and TNF-α in over-activated microglial.
30426288	4	8	theme	BV-2	550:553	arg1	cells					566:570	BV-2 microglial cells	550:570	BV-2 microglial cells stimulated with lipopolysaccharide (LPS)	550:611	The production of NO was determined using nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS).
30426288	5	9	theme	MTT	646:648	arg1	assay					650:654	MTT assay	646:654	MTT assay	646:654	Cell viability was tested using MTT assay.
30426288	9	10	theme	microglial	1534:1543	arg1	cells					1545:1549	LPS- induced BV2 microglial cells	1517:1549	LPS- induced BV2 microglial cells	1517:1549	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	0	11	theme	microglia	110:118	arg1	over-activation					120:134	LPS-induced microglia over-activation	98:134	LPS-induced microglia over-activation	98:134	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	4	12	from	assay	541:545	arg1	cells					566:570	BV-2 microglial cells	550:570	BV-2 microglial cells stimulated with lipopolysaccharide (LPS)	550:611	The production of NO was determined using nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS).
30426288	9	13	theme	induced	1522:1528	arg1	cells					1545:1549	LPS- induced BV2 microglial cells	1517:1549	LPS- induced BV2 microglial cells	1517:1549	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	6	14	theme	real-time	747:755	arg1	qRT-PCR					762:768	qRT-PCR	762:768	qRT-PCR	762:768	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	14	theme	real-time	747:755	arg1	PCR					757:759	quantitative real-time PCR	734:759	quantitative real-time PCR (qRT-PCR)	734:769	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	9	15	theme	microglial	1418:1427	arg1	over-activation					1399:1413	over-activation	1399:1413	over-activation of microglial	1399:1427	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	2	16	theme	effective	360:368	arg1	components					370:379	the effective components	356:379	the effective components	356:379	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	7	17	theme	stilbene-flavane	965:980	arg1	compounds					952:960	two new compounds	944:960	two new compounds	944:960	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	17	theme	stilbene-flavane	965:980	arg1	2					989:989	2	989:989	2	989:989	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	17	theme	stilbene-flavane	965:980	arg1	dimer					982:986	a stilbene-flavane dimer	963:986	a stilbene-flavane dimer (2)	963:990	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	2	18	theme	Chinese	304:310	arg1	Dragon					312:317	Chinese Dragon's	304:319	Chinese Dragon's blood	304:325	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	6	19	theme	quantitative	734:745	arg1	qRT-PCR					762:768	qRT-PCR	762:768	qRT-PCR	762:768	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	19	theme	quantitative	734:745	arg1	PCR					757:759	quantitative real-time PCR	734:759	quantitative real-time PCR (qRT-PCR)	734:769	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	11	20	theme	IL-6	1773:1776	arg1	expressions					1752:1762	the expressions	1748:1762	the expressions of iNOS, IL-6 and TNF-α in over-activated microglial	1748:1815	These could inhibit neuroinflammation by reducing the expressions of iNOS, IL-6 and TNF-α in over-activated microglial.
30426288	4	21	theme	nitrite	533:539	arg1	assay					541:545	nitrite assay	533:545	nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS)	533:611	The production of NO was determined using nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS).
30426288	5	22	theme	Cell	614:617	arg1	viability					619:627	Cell viability	614:627	Cell viability	614:627	Cell viability was tested using MTT assay.
30426288	0	23	theme	Preventive	0:9	arg1	agents					11:16	Preventive agents	0:16	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis	0:86	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	10	24	theme	anti-neuroinflammatory	1640:1661	arg1	effects					1663:1669	the anti-neuroinflammatory effects	1636:1669	the anti-neuroinflammatory effects of Chinese Dragon's blood	1636:1695	Chalcone 9, homoisoflavane 5 and flavone 12-14 are considered to be responsible for the anti-neuroinflammatory effects of Chinese Dragon's blood.
30426288	3	25	theme	Multiple	396:403	arg1	chromatography					405:418	Multiple chromatography	396:418	Multiple chromatography	396:418	Multiple chromatography and spectra analysis were utilized to identify effective constituents.
30426288	2	26	theme	primary	335:341	arg1	mechanism					343:351	the primary mechanism	331:351	the primary mechanism of the effective components	331:379	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	0	27	from	resin	54:58	arg1	diseases					40:47	neurodegenerative diseases	22:47	neurodegenerative diseases from resin of Dracaena cochinchinensis	22:86	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	3	28	theme	spectra	424:430	arg1	analysis					432:439	spectra analysis	424:439	spectra analysis	424:439	Multiple chromatography and spectra analysis were utilized to identify effective constituents.
30426288	6	29	theme	IL-6	794:797	arg1	production					780:789	the production	776:789	the production of IL-6 and TNF-α in the cell supernatants	776:832	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	8	30	theme	tested	1233:1238	arg1	concentration					1240:1252	the tested concentration	1229:1252	the tested concentration	1229:1252	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	3	31	theme	effective	467:475	arg1	constituents					477:488	effective constituents	467:488	effective constituents	467:488	Multiple chromatography and spectra analysis were utilized to identify effective constituents.
30426288	0	32	theme	neurodegenerative	22:38	arg1	diseases					40:47	neurodegenerative diseases	22:47	neurodegenerative diseases from resin of Dracaena cochinchinensis	22:86	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	7	33	theme	known	1040:1044	arg1	compounds					1046:1054	25 known compounds	1037:1054	25 known compounds	1037:1054	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	34	theme	new	948:950	arg1	compounds					952:960	two new compounds	944:960	two new compounds	944:960	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	34	theme	new	948:950	arg1	flavonoid					1006:1014	a quinoid flavonoid	996:1014	a quinoid flavonoid (11)	996:1019	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	34	theme	new	948:950	arg1	dimer					982:986	a stilbene-flavane dimer	963:986	a stilbene-flavane dimer (2)	963:990	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	9	35	theme	iNOS	1467:1470	arg1	cytokines					1489:1497	proinflammatory cytokines IL-6 and TNF-α	1473:1512	proinflammatory cytokines IL-6 and TNF-α	1473:1512	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	35	theme	iNOS	1467:1470	arg1	expressions					1452:1462	the expressions	1448:1462	the expressions of iNOS	1448:1470	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	11	36	theme	iNOS	1767:1770	arg1	expressions					1752:1762	the expressions	1748:1762	the expressions of iNOS, IL-6 and TNF-α in over-activated microglial	1748:1815	These could inhibit neuroinflammation by reducing the expressions of iNOS, IL-6 and TNF-α in over-activated microglial.
30426288	9	37	dep	cytokines	1489:1497	arg1	cytokines					1489:1497	proinflammatory cytokines IL-6 and TNF-α	1473:1512	proinflammatory cytokines IL-6 and TNF-α	1473:1512	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	37	dep	cytokines	1489:1497	arg1	TNF-α					1508:1512	TNF-α	1508:1512	TNF-α	1508:1512	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	37	dep	cytokines	1489:1497	arg1	IL-6					1499:1502	IL-6	1499:1502	IL-6	1499:1502	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	2	38	theme	blood	321:325	arg1	mechanism					343:351	the primary mechanism	331:351	the primary mechanism of the effective components	331:379	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	2	38	theme	blood	321:325	arg1	basis					295:299	the material basis	282:299	the material basis of Chinese Dragon's blood	282:325	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	9	39	theme	BV2	1530:1532	arg1	cells					1545:1549	LPS- induced BV2 microglial cells	1517:1549	LPS- induced BV2 microglial cells	1517:1549	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	1	40	theme	neurodegenerative	232:248	arg1	diseases					250:257	neurodegenerative diseases	232:257	neurodegenerative diseases	232:257	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	8	41	theme	significant	1162:1172	arg1	effects					1197:1203	significant anti-neuroinflammatory effects	1162:1203	significant anti-neuroinflammatory effects	1162:1203	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	9	42	theme	primary	1320:1326	arg1	study					1340:1344	A primary mechanistic study	1318:1344	A primary mechanistic study	1318:1344	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	10	43	theme	Chinese	1674:1680	arg1	Dragon					1682:1687	Chinese Dragon's	1674:1689	Chinese Dragon's blood	1674:1695	Chalcone 9, homoisoflavane 5 and flavone 12-14 are considered to be responsible for the anti-neuroinflammatory effects of Chinese Dragon's blood.
30426288	7	44	theme	D.	916:917	arg1	extract					905:911	the effective extract	891:911	the effective extract of D. cochinchinensis	891:933	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	1	45	theme	diseases	250:257	arg1	therapy					221:227	therapy	221:227	therapy of neurodegenerative diseases	221:257	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	9	46	theme	mechanistic	1328:1338	arg1	study					1340:1344	A primary mechanistic study	1318:1344	A primary mechanistic study	1318:1344	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	4	47	theme	NO	509:510	arg1	production					495:504	The production	491:504	The production of NO	491:510	The production of NO was determined using nitrite assay in BV-2 microglial cells stimulated with lipopolysaccharide (LPS).
30426288	2	48	theme	present	267:273	arg1	study					275:279	the present study	263:279	the present study	263:279	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	7	49	theme	bioassay-directed	859:875	arg1	separation					877:886	The bioassay-directed separation	855:886	The bioassay-directed separation of the effective extract of D. cochinchinensis	855:933	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	9	50	theme	proinflammatory	1473:1487	arg1	expressions					1452:1462	the expressions	1448:1462	the expressions of iNOS	1448:1470	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	50	theme	proinflammatory	1473:1487	arg1	cytokines					1489:1497	proinflammatory cytokines IL-6 and TNF-α	1473:1512	proinflammatory cytokines IL-6 and TNF-α	1473:1512	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	50	theme	proinflammatory	1473:1487	arg1	TNF-α					1508:1512	TNF-α	1508:1512	TNF-α	1508:1512	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	9	50	theme	proinflammatory	1473:1487	arg1	IL-6					1499:1502	IL-6	1499:1502	IL-6	1499:1502	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	8	51	theme	anti-neuroinflammatory	1081:1102	arg1	activities					1104:1113	their anti-neuroinflammatory activities	1075:1113	their anti-neuroinflammatory activities	1075:1113	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	1	52	theme	previous	141:148	arg1	research					150:157	Our previous research	137:157	Our previous research	137:157	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	8	53	theme	anti-neuroinflammatory	1174:1195	arg1	effects					1197:1203	significant anti-neuroinflammatory effects	1162:1203	significant anti-neuroinflammatory effects	1162:1203	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	11	54	from	expressions	1752:1762	arg1	microglial					1806:1815	microglial	1806:1815	microglial	1806:1815	These could inhibit neuroinflammation by reducing the expressions of iNOS, IL-6 and TNF-α in over-activated microglial.
30426288	7	55	theme	quinoid	998:1004	arg1	compounds					952:960	two new compounds	944:960	two new compounds	944:960	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	55	theme	quinoid	998:1004	arg1	flavonoid					1006:1014	a quinoid flavonoid	996:1014	a quinoid flavonoid (11)	996:1019	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	7	55	theme	quinoid	998:1004	arg1	11					1017:1018	11	1017:1018	11	1017:1018	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	6	56	theme	synthase	698:705	arg1	level					666:670	The mRNA level	657:670	The mRNA level of inducible nitric oxide synthase (iNOS)	657:712	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	57	theme	TNF-α	803:807	arg1	production					780:789	the production	776:789	the production of IL-6 and TNF-α in the cell supernatants	776:832	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	10	58	theme	blood	1691:1695	arg1	effects					1663:1669	the anti-neuroinflammatory effects	1636:1669	the anti-neuroinflammatory effects of Chinese Dragon's blood	1636:1695	Chalcone 9, homoisoflavane 5 and flavone 12-14 are considered to be responsible for the anti-neuroinflammatory effects of Chinese Dragon's blood.
30426288	6	59	theme	cell	816:819	arg1	supernatants					821:832	the cell supernatants	812:832	the cell supernatants	812:832	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	60	from	production	780:789	arg1	supernatants					821:832	the cell supernatants	812:832	the cell supernatants	812:832	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	61	theme	oxide	692:696	arg1	iNOS					708:711	iNOS	708:711	iNOS	708:711	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	61	theme	oxide	692:696	arg1	synthase					698:705	inducible nitric oxide synthase	675:705	inducible nitric oxide synthase (iNOS)	675:712	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	3	62	used	utilized	446:453	arg2	analysis					432:439	spectra analysis	424:439	spectra analysis	424:439	Multiple chromatography and spectra analysis were utilized to identify effective constituents.
30426288	3	62	used	utilized	446:453	arg2	chromatography					405:418	Multiple chromatography	396:418	Multiple chromatography	396:418	Multiple chromatography and spectra analysis were utilized to identify effective constituents.
30426288	7	63	dep	D.	916:917	arg1	cochinchinensis					919:933	D. cochinchinensis	916:933	D. cochinchinensis	916:933	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
30426288	2	64	theme	material	286:293	arg1	basis					295:299	the material basis	282:299	the material basis of Chinese Dragon's blood	282:325	In the present study, the material basis of Chinese Dragon's blood and the primary mechanism of the effective components are discussed.
30426288	8	65	theme	positive	1269:1276	arg1	minocycline					1287:1297	minocycline	1287:1297	minocycline (21.87 ± 2.36 µM)	1287:1315	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	8	65	theme	positive	1269:1276	arg1	control					1278:1284	a positive control	1267:1284	a positive control	1267:1284	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	6	66	theme	nitric	685:690	arg1	iNOS					708:711	iNOS	708:711	iNOS	708:711	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	66	theme	nitric	685:690	arg1	synthase					698:705	inducible nitric oxide synthase	675:705	inducible nitric oxide synthase (iNOS)	675:712	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	9	67	theme	effective	1364:1372	arg1	components					1374:1383	the effective components	1360:1383	the effective components	1360:1383	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	0	68	theme	cochinchinensis	72:86	arg1	resin					54:58	resin	54:58	resin of Dracaena cochinchinensis	54:86	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	6	69	theme	inducible	675:683	arg1	iNOS					708:711	iNOS	708:711	iNOS	708:711	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	6	69	theme	inducible	675:683	arg1	synthase					698:705	inducible nitric oxide synthase	675:705	inducible nitric oxide synthase (iNOS)	675:712	The mRNA level of inducible nitric oxide synthase (iNOS) was investigated by quantitative real-time PCR (qRT-PCR), and the production of IL-6 and TNF-α in the cell supernatants was tested by ELISA.
30426288	0	70	theme	Dracaena	63:70	arg1	cochinchinensis					72:86	Dracaena cochinchinensis	63:86	Dracaena cochinchinensis	63:86	Preventive agents for neurodegenerative diseases from resin of Dracaena cochinchinensis attenuate LPS-induced microglia over-activation.
30426288	9	71	dep	induced	1522:1528	arg1	LPS-					1517:1520	LPS-	1517:1520	LPS-	1517:1520	A primary mechanistic study revealed that the effective components could inhibit over-activation of microglial through decreasing the expressions of iNOS, proinflammatory cytokines IL-6 and TNF-α in LPS- induced BV2 microglial cells.
30426288	8	72	from	concentration	1240:1252	arg1	cytotoxities					1213:1224	cytotoxities	1213:1224	cytotoxities at the tested concentration	1213:1252	The evaluation of their anti-neuroinflammatory activities showed that 5, 9, 12, 13, and 14 could exhibit significant anti-neuroinflammatory effects without cytotoxities at the tested concentration, compared to a positive control, minocycline (21.87 ± 2.36 µM).
30426288	1	73	theme	Dracaena	177:184	arg1	cochinchinensis					186:200	Dracaena cochinchinensis	177:200	Dracaena cochinchinensis	177:200	Our previous research revealed resin of Dracaena cochinchinensis as a candidate for therapy of neurodegenerative diseases.
30426288	7	74	theme	effective	895:903	arg1	extract					905:911	the effective extract	891:911	the effective extract of D. cochinchinensis	891:933	The bioassay-directed separation of the effective extract of D. cochinchinensis afforded two new compounds, a stilbene-flavane dimer (2) and a quinoid flavonoid (11), in addition to 25 known compounds.
31593729	5	0	theme	superior	785:792	arg1	efficiency					807:816	the superior transfection efficiency	781:816	the superior transfection efficiency of modified BSA/ plasmid polyplex	781:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	11	1	theme	cationic	1428:1435	arg1	BSA					1437:1439	cationic BSA	1428:1439	cationic BSA	1428:1439	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	5	2	theme	transfection	794:805	arg1	efficiency					807:816	the superior transfection efficiency	781:816	the superior transfection efficiency of modified BSA/ plasmid polyplex	781:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	8	3	theme	polyplex	1066:1073	arg1	colocation					1052:1061	a colocation	1050:1061	a colocation of polyplex with lysosome	1050:1087	Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy.
31593729	10	4	dep	efficiency	1305:1314	arg1	%					1290:1290	%	1290:1290	%	1290:1290	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	3	5	theme	transfection	513:524	arg1	efficiency					526:535	transfection efficiency	513:535	transfection efficiency	513:535	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	11	6	theme	cell	1467:1470	arg1	disruption					1481:1490	no detectable cell membrane disruption	1453:1490	no detectable cell membrane disruption	1453:1490	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	5	7	theme	internalization	753:767	arg1	mechanism					740:748	the mechanism	736:748	the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex	736:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	0	8	dep	in	101:102	arg1	vivo					104:107	vivo	104:107	vivo	104:107	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	10	9	theme	transfection	1292:1303	arg1	efficiency					1305:1314	above than 60% transfection efficiency	1277:1314	above than 60% transfection efficiency	1277:1314	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	10	10	theme	cell	1259:1262	arg1	lines					1264:1268	Four non-adherent cell lines	1241:1268	Four non-adherent cell lines	1241:1268	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	0	11	theme	polyplex	86:93	arg1	fate					95:98	polyplex fate	86:98	polyplex fate	86:98	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	11	theme	polyplex	86:93	arg1	vector					55:60	plasmid DNA delivery vector	34:60	plasmid DNA delivery vector	34:60	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	7	12	theme	significant	994:1004	arg1	role					1006:1009	a significant role	992:1009	a significant role	992:1009	The caveolae-mediated and lipid-mediated pathways play a significant role in the polyplex internalization.
31593729	1	13	theme	Cationic	124:131	arg1	macromolecules					133:146	Cationic macromolecules	124:146	Cationic macromolecules	124:146	Cationic macromolecules condense DNA into small nanoparticles and form polyplex.
31593729	0	14	theme	in	101:102	arg1	compatibility					109:121	in vivo compatibility	101:121	in vivo compatibility	101:121	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	14	theme	in	101:102	arg1	vector					55:60	plasmid DNA delivery vector	34:60	plasmid DNA delivery vector	34:60	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	6	15	theme	cell	925:928	arg1	lines					930:934	two adherent cell lines	912:934	two adherent cell lines	912:934	The effect of specific endocytic inhibitors was studied in two adherent cell lines.
31593729	11	16	theme	hemolytic	1379:1387	arg1	effect					1389:1394	no significant hemolytic effect	1364:1394	no significant hemolytic effect	1364:1394	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	2	17	theme	intracellular	275:287	arg1	routing					289:295	the intracellular routing	271:295	the intracellular routing	271:295	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	1	18	theme	small	166:170	arg1	nanoparticles					172:184	small nanoparticles	166:184	small nanoparticles	166:184	Cationic macromolecules condense DNA into small nanoparticles and form polyplex.
31593729	0	19	theme	delivery	46:53	arg1	fate					95:98	polyplex fate	86:98	polyplex fate	86:98	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	19	theme	delivery	46:53	arg1	compatibility					109:121	in vivo compatibility	101:121	in vivo compatibility	101:121	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	19	theme	delivery	46:53	arg1	vector					55:60	plasmid DNA delivery vector	34:60	plasmid DNA delivery vector	34:60	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	6	20	theme	adherent	916:923	arg1	lines					930:934	two adherent cell lines	912:934	two adherent cell lines	912:934	The effect of specific endocytic inhibitors was studied in two adherent cell lines.
31593729	0	21	theme	DNA	42:44	arg1	fate					95:98	polyplex fate	86:98	polyplex fate	86:98	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	21	theme	DNA	42:44	arg1	compatibility					109:121	in vivo compatibility	101:121	in vivo compatibility	101:121	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	21	theme	DNA	42:44	arg1	vector					55:60	plasmid DNA delivery vector	34:60	plasmid DNA delivery vector	34:60	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	9	22	dep	70	1170:1171	arg1	to					1167:1168	to	1167:1168	to	1167:1168	Up to 70% of polyplex successfully escaped the lysosome without degradation.
31593729	3	23	theme	oligochitosan-modified	339:360	arg1	vectors					362:368	oligochitosan-modified vectors	339:368	oligochitosan-modified vectors with different protein moieties	339:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	3	23	theme	oligochitosan-modified	339:360	arg1	vector					428:433	gene delivery vector	414:433	gene delivery vector	414:433	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	5	24	theme	plasmid	835:841	arg1	polyplex					843:850	modified BSA/ plasmid polyplex	821:850	modified BSA/ plasmid polyplex	821:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	11	25	theme	BSA	1437:1439	arg1	500 μg/mL					1415:1423	500 μg/mL	1415:1423	500 μg/mL of cationic BSA	1415:1439	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	11	26	dep	500 μg/mL	1415:1423	arg1	up					1409:1410	up	1409:1410	up	1409:1410	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	4	27	theme	modified	667:674	arg1	zein					676:679	zein	676:679	zein	676:679	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	10	28	theme	optimized	1322:1330	arg1	ratio					1347:1351	an optimized vector/plasmid ratio	1319:1351	an optimized vector/plasmid ratio	1319:1351	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	5	29	theme	polyplex	843:850	arg1	efficiency					807:816	the superior transfection efficiency	781:816	the superior transfection efficiency of modified BSA/ plasmid polyplex	781:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	4	30	theme	oligochitosan-modified	566:587	arg1	BSA					611:613	BSA	611:613	BSA	611:613	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	4	30	theme	oligochitosan-modified	566:587	arg1	albumin					602:608	oligochitosan-modified bovine serum albumin	566:608	oligochitosan-modified bovine serum albumin (BSA)	566:614	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	11	31	theme	detectable	1456:1465	arg1	disruption					1481:1490	no detectable cell membrane disruption	1453:1490	no detectable cell membrane disruption	1453:1490	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	7	32	theme	lipid-mediated	963:976	arg1	pathways					978:985	The caveolae-mediated and lipid-mediated pathways	937:985	The caveolae-mediated and lipid-mediated pathways	937:985	The caveolae-mediated and lipid-mediated pathways play a significant role in the polyplex internalization.
31593729	12	33	theme	intracellular	1542:1554	arg1	delivery					1556:1563	good intracellular delivery	1537:1563	good intracellular delivery	1537:1563	Overall, the hybrid biomacromolecule showed good intracellular delivery and safety in a mice model.
31593729	3	34	theme	protein	385:391	arg1	moieties					393:400	different protein moieties	375:400	different protein moieties	375:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	10	35	dep	%	1290:1290	arg1	60					1288:1289	60	1288:1289	60	1288:1289	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	4	36	theme	serum	596:600	arg1	BSA					611:613	BSA	611:613	BSA	611:613	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	4	36	theme	serum	596:600	arg1	albumin					602:608	oligochitosan-modified bovine serum albumin	566:608	oligochitosan-modified bovine serum albumin (BSA)	566:614	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	3	37	theme	delivery	419:426	arg1	vectors					362:368	oligochitosan-modified vectors	339:368	oligochitosan-modified vectors with different protein moieties	339:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	3	37	theme	delivery	419:426	arg1	vector					428:433	gene delivery vector	414:433	gene delivery vector	414:433	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	4	38	theme	bovine	589:594	arg1	BSA					611:613	BSA	611:613	BSA	611:613	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	4	38	theme	bovine	589:594	arg1	albumin					602:608	oligochitosan-modified bovine serum albumin	566:608	oligochitosan-modified bovine serum albumin (BSA)	566:614	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	3	39	used	used	406:409	arg2	vector					428:433	gene delivery vector	414:433	gene delivery vector	414:433	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	3	39	used	used	406:409	arg2	vectors					362:368	oligochitosan-modified vectors	339:368	oligochitosan-modified vectors with different protein moieties	339:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	0	40	dep	vector	55:60	arg1	trafficking					73:83	Endocytic trafficking	63:83	Endocytic trafficking	63:83	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	12	41	theme	good	1537:1540	arg1	delivery					1556:1563	good intracellular delivery	1537:1563	good intracellular delivery	1537:1563	Overall, the hybrid biomacromolecule showed good intracellular delivery and safety in a mice model.
31593729	3	42	theme	protein	452:458	arg1	moiety					460:465	protein moiety	452:465	protein moiety	452:465	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	0	43	theme	plasmid	34:40	arg1	fate					95:98	polyplex fate	86:98	polyplex fate	86:98	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	43	theme	plasmid	34:40	arg1	compatibility					109:121	in vivo compatibility	101:121	in vivo compatibility	101:121	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	0	43	theme	plasmid	34:40	arg1	vector					55:60	plasmid DNA delivery vector	34:60	plasmid DNA delivery vector	34:60	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	12	44	theme	mice	1581:1584	arg1	model					1586:1590	a mice model	1579:1590	a mice model	1579:1590	Overall, the hybrid biomacromolecule showed good intracellular delivery and safety in a mice model.
31593729	2	45	theme	polyplex	228:235	arg1	composition					209:219	The composition	205:219	The composition of the polyplex	205:235	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	3	46	theme	moiety	460:465	arg1	types					443:447	the types	439:447	the types of protein moiety	439:465	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	5	47	theme	modified	821:828	arg1	polyplex					843:850	modified BSA/ plasmid polyplex	821:850	modified BSA/ plasmid polyplex	821:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	5	48	theme	BSA/	830:833	arg1	polyplex					843:850	modified BSA/ plasmid polyplex	821:850	modified BSA/ plasmid polyplex	821:850	These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex.
31593729	6	49	theme	inhibitors	886:895	arg1	effect					857:862	The effect	853:862	The effect of specific endocytic inhibitors	853:895	The effect of specific endocytic inhibitors was studied in two adherent cell lines.
31593729	8	50	with	colocation	1052:1061	arg1	lysosome					1080:1087	lysosome	1080:1087	lysosome	1080:1087	Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy.
31593729	3	51	theme	escape	494:499	arg1	ability					501:507	the endosome escape ability	481:507	the endosome escape ability	481:507	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	3	52	theme	gene	414:417	arg1	vectors					362:368	oligochitosan-modified vectors	339:368	oligochitosan-modified vectors with different protein moieties	339:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	3	52	theme	gene	414:417	arg1	vector					428:433	gene delivery vector	414:433	gene delivery vector	414:433	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	6	53	theme	endocytic	876:884	arg1	inhibitors					886:895	specific endocytic inhibitors	867:895	specific endocytic inhibitors	867:895	The effect of specific endocytic inhibitors was studied in two adherent cell lines.
31593729	11	54	theme	significant	1367:1377	arg1	effect					1389:1394	no significant hemolytic effect	1364:1394	no significant hemolytic effect	1364:1394	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	7	55	theme	caveolae-mediated	941:957	arg1	pathways					978:985	The caveolae-mediated and lipid-mediated pathways	937:985	The caveolae-mediated and lipid-mediated pathways	937:985	The caveolae-mediated and lipid-mediated pathways play a significant role in the polyplex internalization.
31593729	4	56	theme	modified	548:555	arg1	vectors					557:563	the modified vectors	544:563	the modified vectors	544:563	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	8	57	theme	confocal	1143:1150	arg1	microscopy					1152:1161	confocal microscopy	1143:1161	confocal microscopy	1143:1161	Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy.
31593729	6	58	theme	specific	867:874	arg1	inhibitors					886:895	specific endocytic inhibitors	867:895	specific endocytic inhibitors	867:895	The effect of specific endocytic inhibitors was studied in two adherent cell lines.
31593729	3	59	with	vectors	362:368	arg1	moieties					393:400	different protein moieties	375:400	different protein moieties	375:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	10	60	theme	non-adherent	1246:1257	arg1	lines					1264:1268	Four non-adherent cell lines	1241:1268	Four non-adherent cell lines	1241:1268	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	4	61	theme	90	623:624	arg1	%					625:625	%	625:625	%	625:625	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	9	62	theme	polyplex	1177:1184	arg1	polyplex					1177:1184	polyplex	1177:1184	polyplex	1177:1184	Up to 70% of polyplex successfully escaped the lysosome without degradation.
31593729	9	62	theme	polyplex	1177:1184	arg1	%					1172:1172	Up to 70%	1164:1172	Up to 70% of polyplex	1164:1184	Up to 70% of polyplex successfully escaped the lysosome without degradation.
31593729	11	63	theme	membrane	1472:1479	arg1	disruption					1481:1490	no detectable cell membrane disruption	1453:1490	no detectable cell membrane disruption	1453:1490	Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption.
31593729	2	64	theme	polyplex	317:324	arg1	routing					289:295	the intracellular routing	271:295	the intracellular routing	271:295	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	2	64	theme	polyplex	317:324	arg1	fate					305:308	the fate	301:308	the fate of the polyplex	301:324	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	2	64	theme	polyplex	317:324	arg1	process					262:268	the endocytic process	248:268	the endocytic process	248:268	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	10	65	theme	vector/plasmid	1332:1345	arg1	ratio					1347:1351	an optimized vector/plasmid ratio	1319:1351	an optimized vector/plasmid ratio	1319:1351	Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio.
31593729	0	66	theme	Endocytic	63:71	arg1	trafficking					73:83	Endocytic trafficking	63:83	Endocytic trafficking	63:83	Oligochitosan modified albumin as plasmid DNA delivery vector: Endocytic trafficking, polyplex fate, in vivo compatibility.
31593729	4	67	dep	showed	616:621	arg1	compared					651:658	compared	651:658	showed 90% transfection efficeincy compared to the modified zein and ovalbumin	616:693	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	2	68	theme	endocytic	252:260	arg1	process					262:268	the endocytic process	248:268	the endocytic process	248:268	The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex.
31593729	3	69	theme	endosome	485:492	arg1	ability					501:507	the endosome escape ability	481:507	the endosome escape ability	481:507	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31593729	4	70	theme	transfection	627:638	arg1	efficeincy					640:649	90% transfection efficeincy	623:649	90% transfection efficeincy	623:649	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	8	71	dep	LysoTracker	1125:1135	arg1	the					1109:1111	the	1109:1111	the	1109:1111	Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy.
31593729	8	71	dep	LysoTracker	1125:1135	arg1	presence					1113:1120	presence	1113:1120	presence	1113:1120	Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy.
31593729	12	72	theme	hybrid	1506:1511	arg1	biomacromolecule					1513:1528	the hybrid biomacromolecule	1502:1528	the hybrid biomacromolecule	1502:1528	Overall, the hybrid biomacromolecule showed good intracellular delivery and safety in a mice model.
31593729	4	73	theme	%	625:625	arg1	efficeincy					640:649	90% transfection efficeincy	623:649	90% transfection efficeincy	623:649	Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin.
31593729	7	74	theme	polyplex	1018:1025	arg1	internalization					1027:1041	the polyplex internalization	1014:1041	the polyplex internalization	1014:1041	The caveolae-mediated and lipid-mediated pathways play a significant role in the polyplex internalization.
31593729	3	75	theme	different	375:383	arg1	moieties					393:400	different protein moieties	375:400	different protein moieties	375:400	Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency.
31857176	5	0	theme	CHn-KAm	801:807	arg1	films					809:813	CHn-KAm films	801:813	CHn-KAm films	801:813	The mechanical properties of CHn-KAm films increased significantly with kojic acid addition into chitosan.
31857176	3	1	theme	water	527:531	arg1	permeability					539:550	water vapor permeability	527:550	water vapor permeability	527:550	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	1	theme	water	527:531	arg1	viscosity					501:509	apparent viscosity	492:509	apparent viscosity	492:509	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	2	2	with	chitosan	302:309	arg1	weights					333:339	higher molecular weights	316:339	higher molecular weights	316:339	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	0	3	theme	biodegradable	68:80	arg1	films					82:86	composite biodegradable films	58:86	composite biodegradable films	58:86	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	2	4	theme	viscosity	358:366	arg1	permeability					413:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	1	5	theme	different	246:254	arg1	ratios					256:261	different ratios	246:261	different ratios	246:261	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	0	6	theme	active	92:97	arg1	materials					109:117	active packaging materials	92:117	active packaging materials	92:117	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	6	7	theme	spectral	884:891	arg1	analyses					893:900	FTIR spectral analyses	879:900	FTIR spectral analyses	879:900	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	4	8	theme	*	688:688	arg1	value					734:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	9	theme	decreased	721:729	arg1	*					732:732	a decreased L*	719:732	a decreased L*	719:732	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	6	10	theme	FTIR	879:882	arg1	analyses					893:900	FTIR spectral analyses	879:900	FTIR spectral analyses	879:900	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	4	11	theme	value	734:738	arg1	levels					677:682	increased levels	667:682	increased levels of a*, b*, ΔE*, opacity values and a decreased L* value	667:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	12	theme	*	697:697	arg1	value					734:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	1	13	theme	kojic	133:137	arg1	acid					139:142	kojic acid	133:142	kojic acid	133:142	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	4	14	theme	ΔE	695:696	arg1	*					697:697	ΔE*	695:697	ΔE*	695:697	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	15	theme	opacity	700:706	arg1	values					708:713	opacity values	700:713	opacity values	700:713	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	5	16	theme	films	809:813	arg1	properties					787:796	The mechanical properties	772:796	The mechanical properties of CHn-KAm films	772:813	The mechanical properties of CHn-KAm films increased significantly with kojic acid addition into chitosan.
31857176	3	17	theme	kojic	440:444	arg1	acid					446:449	kojic acid	440:449	kojic acid addition	440:458	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	2	18	theme	vapor	407:411	arg1	permeability					413:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	0	19	theme	packaging	99:107	arg1	materials					109:117	active packaging materials	92:117	active packaging materials	92:117	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	6	20	with	consistent	951:960	arg1	mechanical					982:991	mechanical	982:991	mechanical	982:991	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	6	20	with	consistent	951:960	arg1	properties					1006:1015	composite film mechanical and physical properties	967:1015	properties	1006:1015	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	2	21	theme	water	401:405	arg1	vapor					407:411	lower water vapor	395:411	lower water vapor	395:411	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	6	22	theme	film	939:942	arg1	matrix					944:949	the film matrix	935:949	the film matrix	935:949	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	8	23	theme	higher	1138:1143	arg1	weight					1155:1160	a higher molecular weight	1136:1160	a higher molecular weight as CHn-KAm films	1136:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	2	24	theme	higher	316:321	arg1	weights					333:339	higher molecular weights	316:339	higher molecular weights	316:339	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	2	25	theme	lower	395:399	arg1	vapor					407:411	lower water vapor	395:411	lower water vapor	395:411	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	5	26	theme	mechanical	776:785	arg1	properties					787:796	The mechanical properties	772:796	The mechanical properties of CHn-KAm films	772:813	The mechanical properties of CHn-KAm films increased significantly with kojic acid addition into chitosan.
31857176	4	27	theme	values	708:713	arg1	value					734:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	5	28	theme	kojic	844:848	arg1	addition					855:862	kojic acid addition	844:862	kojic acid addition into chitosan	844:876	The mechanical properties of CHn-KAm films increased significantly with kojic acid addition into chitosan.
31857176	3	29	theme	acid	446:449	arg1	addition					451:458	kojic acid addition	440:458	kojic acid addition	440:458	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	2	30	theme	mechanical	369:378	arg1	properties					380:389	mechanical properties	369:389	mechanical properties	369:389	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	3	31	theme	antibacterial	578:590	arg1	activity					592:599	the antibacterial activity	574:599	the antibacterial activity of the films	574:612	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	32	theme	apparent	492:499	arg1	content					518:524	water content	512:524	water content	512:524	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	32	theme	apparent	492:499	arg1	permeability					539:550	water vapor permeability	527:550	water vapor permeability	527:550	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	32	theme	apparent	492:499	arg1	viscosity					501:509	apparent viscosity	492:509	apparent viscosity	492:509	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	33	theme	vapor	533:537	arg1	permeability					539:550	water vapor permeability	527:550	water vapor permeability	527:550	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	33	theme	vapor	533:537	arg1	viscosity					501:509	apparent viscosity	492:509	apparent viscosity	492:509	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	1	34	theme	acid	139:142	arg1	CHn-KAm					161:167	CHn-KAm	161:167	CHn-KAm	161:167	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	1	34	theme	acid	139:142	arg1	films					154:158	Chitosan and kojic acid composite films	120:158	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios	120:261	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	0	35	theme	kojic	34:38	arg1	acid					40:43	new kojic acid	30:43	new kojic acid	30:43	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	4	36	theme	b	691:691	arg1	*					692:692	b*	691:692	b*	691:692	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	7	37	theme	antibacterial	1055:1067	arg1	effectiveness					1069:1081	significant antibacterial effectiveness	1043:1081	significant antibacterial effectiveness	1043:1081	The CHn-KAm films showed significant antibacterial effectiveness.
31857176	0	38	theme	new	30:32	arg1	acid					40:43	new kojic acid	30:43	new kojic acid	30:43	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	5	39	theme	acid	850:853	arg1	addition					855:862	kojic acid addition	844:862	kojic acid addition into chitosan	844:876	The mechanical properties of CHn-KAm films increased significantly with kojic acid addition into chitosan.
31857176	8	40	theme	molecular	1145:1153	arg1	weight					1155:1160	a higher molecular weight	1136:1160	a higher molecular weight as CHn-KAm films	1136:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	0	41	dep	acid	40:43	arg1	films					82:86	composite biodegradable films	58:86	composite biodegradable films	58:86	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	1	42	theme	molecular	210:218	arg1	weights					220:226	molecular weights	210:226	molecular weights	210:226	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	8	43	theme	food	1222:1225	arg1	packaging					1227:1235	food packaging	1222:1235	food packaging	1222:1235	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	8	44	theme	CHn-KAm	1165:1171	arg1	films					1173:1177	CHn-KAm films	1165:1177	CHn-KAm films	1165:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	6	45	from	interaction	920:930	arg1	matrix					944:949	the film matrix	935:949	the film matrix	935:949	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	3	46	theme	films	608:612	arg1	activity					592:599	the antibacterial activity	574:599	the antibacterial activity of the films	574:612	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	2	47	theme	higher	351:356	arg1	permeability					413:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	0	48	theme	acid	40:43	arg1	properties					16:25	properties	16:25	properties	16:25	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	0	48	theme	acid	40:43	arg1	Development					0:10	Development	0:10	Development	0:10	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	7	49	theme	significant	1043:1053	arg1	effectiveness					1069:1081	significant antibacterial effectiveness	1043:1081	significant antibacterial effectiveness	1043:1081	The CHn-KAm films showed significant antibacterial effectiveness.
31857176	8	50	theme	promising	1199:1207	arg1	material					1209:1216	a promising material	1197:1216	a promising material for food packaging	1197:1235	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	8	50	theme	promising	1199:1207	arg1	use					1115:1117	the use	1111:1117	the use of chitosan with a higher molecular weight as CHn-KAm films	1111:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	1	51	theme	Chitosan	120:127	arg1	CHn-KAm					161:167	CHn-KAm	161:167	CHn-KAm	161:167	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	1	51	theme	Chitosan	120:127	arg1	films					154:158	Chitosan and kojic acid composite films	120:158	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios	120:261	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	6	52	theme	strong	913:918	arg1	interaction					920:930	a strong interaction	911:930	a strong interaction in the film matrix	911:949	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
31857176	7	53	theme	CHn-KAm	1022:1028	arg1	films					1030:1034	The CHn-KAm films	1018:1034	The CHn-KAm films	1018:1034	The CHn-KAm films showed significant antibacterial effectiveness.
31857176	4	54	theme	chitosan	756:763	arg1	films					765:769	chitosan films	756:769	chitosan films	756:769	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	55	theme	*	692:692	arg1	value					734:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	56	theme	*	732:732	arg1	value					734:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	a*, b*, ΔE*, opacity values and a decreased L* value	687:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	4	57	theme	acid	636:639	arg1	concentration					641:653	the kojic acid concentration	626:653	the kojic acid concentration	626:653	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	8	58	theme	chitosan	1122:1129	arg1	material					1209:1216	a promising material	1197:1216	a promising material for food packaging	1197:1235	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	8	58	theme	chitosan	1122:1129	arg1	use					1115:1117	the use	1111:1117	the use of chitosan with a higher molecular weight as CHn-KAm films	1111:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	4	59	theme	L	731:731	arg1	*					732:732	a decreased L*	719:732	a decreased L*	719:732	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	2	60	theme	molecular	323:331	arg1	weights					333:339	higher molecular weights	316:339	higher molecular weights	316:339	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	4	61	theme	kojic	630:634	arg1	concentration					641:653	the kojic acid concentration	626:653	the kojic acid concentration	626:653	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	0	62	theme	composite	58:66	arg1	films					82:86	composite biodegradable films	58:86	composite biodegradable films	58:86	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	8	63	with	use	1115:1117	arg1	weight					1155:1160	a higher molecular weight	1136:1160	a higher molecular weight as CHn-KAm films	1136:1177	The results suggested that the use of chitosan with a higher molecular weight as CHn-KAm films is expected to be a promising material for food packaging.
31857176	4	64	theme	increased	667:675	arg1	levels					677:682	increased levels	667:682	increased levels of a*, b*, ΔE*, opacity values and a decreased L* value	667:738	Increasing the kojic acid concentration resulted in increased levels of a*, b*, ΔE*, opacity values and a decreased L* value, as compared to chitosan films.
31857176	2	65	theme	properties	380:389	arg1	permeability					413:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	higher viscosity, mechanical properties and lower water vapor permeability	351:424	Results showed that chitosan with higher molecular weights presented higher viscosity, mechanical properties and lower water vapor permeability.
31857176	3	66	theme	water	512:516	arg1	content					518:524	water content	512:524	water content	512:524	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	3	66	theme	water	512:516	arg1	viscosity					501:509	apparent viscosity	492:509	apparent viscosity	492:509	Furthermore, kojic acid addition further significantly decreased apparent viscosity, water content, water vapor permeability as well as increasing the antibacterial activity of the films.
31857176	1	67	theme	kojic	232:236	arg1	acid					238:241	kojic acid	232:241	kojic acid	232:241	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	0	68	theme	chitosan	49:56	arg1	properties					16:25	properties	16:25	properties	16:25	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	0	68	theme	chitosan	49:56	arg1	Development					0:10	Development	0:10	Development	0:10	Development and properties of new kojic acid and chitosan composite biodegradable films for active packaging materials.
31857176	1	69	theme	composite	144:152	arg1	CHn-KAm					161:167	CHn-KAm	161:167	CHn-KAm	161:167	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	1	69	theme	composite	144:152	arg1	films					154:158	Chitosan and kojic acid composite films	120:158	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios	120:261	Chitosan and kojic acid composite films (CHn-KAm) utilizing three chitosan with differing molecular weights and kojic acid in different ratios were investigated.
31857176	6	70	theme	physical	997:1004	arg1	properties					1006:1015	composite film mechanical and physical properties	967:1015	properties	1006:1015	FTIR spectral analyses revealed a strong interaction in the film matrix consistent with composite film mechanical and physical properties.
30267815	3	0	from	effect	507:512	arg1	stability					690:698	the stability	686:698	the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	686:763	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	2	1	dep	formation	329:337	arg1	the					325:327	the	325:327	the	325:327	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	5	2	theme	phase	961:965	arg1	separation					967:976	no phase separation	958:976	no phase separation	958:976	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	1	3	theme	environmental	214:226	arg1	pH					250:251	low pH	246:251	low pH	246:251	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	3	theme	environmental	214:226	arg1	stresses					228:235	certain environmental stresses	206:235	certain environmental stresses	206:235	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	3	theme	environmental	214:226	arg1	strength					272:279	moderate ionic strength	257:279	moderate ionic strength (>100 mmol/L NaCl)	257:298	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	3	4	dep	stresses	634:641	arg1	etc.					677:680	etc.	677:680	etc.	677:680	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	4	dep	stresses	634:641	arg1	temperature					648:658	temperature	648:658	temperature	648:658	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	4	dep	stresses	634:641	arg1	strength					667:674	ionic strength	661:674	ionic strength	661:674	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	4	dep	stresses	634:641	arg1	pH					644:645	pH	644:645	pH	644:645	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	5	theme	emulsifier	584:593	arg1	ratio					560:564	OSAS/phosphatidylcholine weight ratio	528:564	OSAS/phosphatidylcholine weight ratio	528:564	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	5	theme	emulsifier	584:593	arg1	concentration					595:607	emulsifier concentration	584:607	emulsifier concentration	584:607	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	4	6	theme	weight	831:836	arg1	ratio = 3:7					838:848	OSAS/phosphatidylcholine weight ratio = 3:7	806:848	OSAS/phosphatidylcholine weight ratio = 3:7	806:848	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	4	6	theme	weight	831:836	arg1	OSAS					800:803	OSAS	800:803	OSAS (OSAS/phosphatidylcholine weight ratio = 3:7)	800:849	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	2	7	theme	octenyl	356:362	arg1	starch					392:397	octenyl succinic anhydride-modified starch	356:397	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil	356:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	2	7	theme	octenyl	356:362	arg1	OSAS					400:403	OSAS	400:403	OSAS	400:403	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	5	8	theme	certain	1023:1029	arg1	degree					1031:1036	a certain degree	1021:1036	a certain degree of steric repulsion that protects the nanoemulsion from oiling-off	1021:1103	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	7	9	theme	electron	1335:1342	arg1	microscopy					1344:1353	Transmission electron microscopy images	1322:1360	Transmission electron microscopy images	1322:1360	Transmission electron microscopy images showed that OSAS and phosphatidylcholine were both adsorbed around the surface of the emulsion.
30267815	1	10	theme	certain	206:212	arg1	pH					250:251	low pH	246:251	low pH	246:251	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	10	theme	certain	206:212	arg1	stresses					228:235	certain environmental stresses	206:235	certain environmental stresses	206:235	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	10	theme	certain	206:212	arg1	strength					272:279	moderate ionic strength	257:279	moderate ionic strength (>100 mmol/L NaCl)	257:298	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	6	11	from	90 °C	1254:1258	arg1	≤800 mmol/L					1213:1223	≤800 mmol/L	1213:1223	≤800 mmol/L	1213:1223	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	11	from	90 °C	1254:1258	arg1	temperatures					1230:1241	temperatures	1230:1241	temperatures from 30 to 90 °C	1230:1258	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	11	from	90 °C	1254:1258	arg1	concentrations					1197:1210	high NaCl concentrations	1187:1210	high NaCl concentrations (≤800 mmol/L)	1187:1224	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	1	12	theme	phase	183:187	arg1	separation					189:198	phase separation	183:198	phase separation	183:198	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	4	13	theme	OSAS/phosphatidylcholine	806:829	arg1	ratio = 3:7					838:848	OSAS/phosphatidylcholine weight ratio = 3:7	806:848	OSAS/phosphatidylcholine weight ratio = 3:7	806:848	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	4	13	theme	OSAS/phosphatidylcholine	806:829	arg1	OSAS					800:803	OSAS	800:803	OSAS (OSAS/phosphatidylcholine weight ratio = 3:7)	800:849	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	6	14	theme	phosphatidylcholine-enhanced	1110:1137	arg1	stable					1177:1182	stable	1177:1182	stable	1177:1182	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	14	theme	phosphatidylcholine-enhanced	1110:1137	arg1	nanoemulsions					1139:1151	The phosphatidylcholine-enhanced nanoemulsions	1106:1151	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	1106:1170	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	15	theme	high	1187:1190	arg1	≤800 mmol/L					1213:1223	≤800 mmol/L	1213:1223	≤800 mmol/L	1213:1223	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	15	theme	high	1187:1190	arg1	concentrations					1197:1210	high NaCl concentrations	1187:1210	high NaCl concentrations (≤800 mmol/L)	1187:1224	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	5	16	dep	OSAS	1012:1015	arg1	has					1017:1019	has	1017:1019	has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off	1017:1103	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	5	17	theme	repulsion	1048:1056	arg1	degree					1031:1036	a certain degree	1021:1036	a certain degree of steric repulsion that protects the nanoemulsion from oiling-off	1021:1103	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	1	18	theme	Phosphatidylcholine-coated	120:145	arg1	emulsions					147:155	Phosphatidylcholine-coated emulsions	120:155	Phosphatidylcholine-coated emulsions	120:155	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	3	19	theme	nanoemulsions	732:744	arg1	stability					690:698	the stability	686:698	the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	686:763	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	6	20	from	temperatures	1230:1241	arg1	stable					1177:1182	stable	1177:1182	stable	1177:1182	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	20	from	temperatures	1230:1241	arg1	nanoemulsions					1139:1151	The phosphatidylcholine-enhanced nanoemulsions	1106:1151	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	1106:1170	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	8	21	dep	formation	1514:1522	arg1	the					1510:1512	the	1510:1512	the	1510:1512	These results showed that adding OSAS could improve the formation and stability of phosphatidylcholine-stabilized emulsions.
30267815	3	22	theme	environmental	620:632	arg1	stresses					634:641	environmental stresses	620:641	environmental stresses (pH, temperature, ionic strength, etc.)	620:681	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	2	23	theme	soybean	490:496	arg1	oil					498:500	soybean oil	490:500	soybean oil	490:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	2	24	theme	oil	498:500	arg1	encapsulation					473:485	the encapsulation	469:485	the encapsulation of soybean oil	469:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	8	25	theme	emulsions	1572:1580	arg1	stability					1528:1536	stability	1528:1536	stability	1528:1536	These results showed that adding OSAS could improve the formation and stability of phosphatidylcholine-stabilized emulsions.
30267815	8	25	theme	emulsions	1572:1580	arg1	formation					1514:1522	formation	1514:1522	formation	1514:1522	These results showed that adding OSAS could improve the formation and stability of phosphatidylcholine-stabilized emulsions.
30267815	3	26	theme	stresses	634:641	arg1	effect					507:512	The effect	503:512	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	503:763	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	27	theme	OSAS/phosphatidylcholine	528:551	arg1	concentration					595:607	emulsifier concentration	584:607	emulsifier concentration	584:607	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	27	theme	OSAS/phosphatidylcholine	528:551	arg1	ratio					560:564	OSAS/phosphatidylcholine weight ratio	528:564	OSAS/phosphatidylcholine weight ratio	528:564	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	27	theme	OSAS/phosphatidylcholine	528:551	arg1	composition					571:581	oil composition	567:581	oil composition	567:581	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	27	theme	OSAS/phosphatidylcholine	528:551	arg1	etc.					610:613	etc.	610:613	etc.	610:613	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	0	28	theme	nanoemulsions	56:68	arg1	stability					14:22	the stability	10:22	the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch	10:117	Improving the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch.
30267815	3	29	theme	oil	567:569	arg1	ratio					560:564	OSAS/phosphatidylcholine weight ratio	528:564	OSAS/phosphatidylcholine weight ratio	528:564	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	29	theme	oil	567:569	arg1	composition					571:581	oil composition	567:581	oil composition	567:581	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	8	30	theme	phosphatidylcholine-stabilized	1541:1570	arg1	emulsions					1572:1580	phosphatidylcholine-stabilized emulsions	1541:1580	phosphatidylcholine-stabilized emulsions	1541:1580	These results showed that adding OSAS could improve the formation and stability of phosphatidylcholine-stabilized emulsions.
30267815	4	31	theme	nanoemulsion	889:900	arg1	formation					862:870	the formation	858:870	the formation of a more stable nanoemulsion	858:900	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	0	32	theme	phosphatidylcholine-enhanced	27:54	arg1	nanoemulsions					56:68	phosphatidylcholine-enhanced nanoemulsions	27:68	phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch	27:117	Improving the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch.
30267815	4	33	theme	Adequate	779:786	arg1	addition					788:795	Adequate addition	779:795	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7)	779:849	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	3	34	theme	ionic	661:665	arg1	strength					667:674	ionic strength	661:674	ionic strength	661:674	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	34	theme	ionic	661:665	arg1	pH					644:645	pH	644:645	pH	644:645	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	5	35	from	pH 2	918:921	arg1	emulsion					906:913	An emulsion	903:913	An emulsion at pH 2	903:921	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	5	35	from	pH 2	918:921	arg1	unstable					927:934	unstable	927:934	unstable	927:934	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	1	36	theme	moderate	257:264	arg1	strength					272:279	moderate ionic strength	257:279	moderate ionic strength (>100 mmol/L NaCl)	257:298	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	36	theme	moderate	257:264	arg1	NaCl					294:297	>100 mmol/L NaCl	282:297	>100 mmol/L NaCl	282:297	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	0	37	theme	octenyl	76:82	arg1	starch					112:117	octenyl succinic anhydride-modified starch	76:117	octenyl succinic anhydride-modified starch	76:117	Improving the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch.
30267815	6	38	theme	electrostatic	1296:1308	arg1	repulsions					1310:1319	steric and electrostatic repulsions	1285:1319	steric and electrostatic repulsions	1285:1319	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	39	theme	NaCl	1192:1195	arg1	≤800 mmol/L					1213:1223	≤800 mmol/L	1213:1223	≤800 mmol/L	1213:1223	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	39	theme	NaCl	1192:1195	arg1	concentrations					1197:1210	high NaCl concentrations	1187:1210	high NaCl concentrations (≤800 mmol/L)	1187:1224	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	2	40	theme	starch	392:397	arg1	nanoemulsions					417:429	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions	356:429	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil	356:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	4	41	theme	OSAS	800:803	arg1	addition					788:795	Adequate addition	779:795	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7)	779:849	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	6	42	from	concentrations	1197:1210	arg1	stable					1177:1182	stable	1177:1182	stable	1177:1182	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	42	from	concentrations	1197:1210	arg1	nanoemulsions					1139:1151	The phosphatidylcholine-enhanced nanoemulsions	1106:1151	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	1106:1170	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	6	43	theme	steric	1285:1290	arg1	repulsions					1310:1319	steric and electrostatic repulsions	1285:1319	steric and electrostatic repulsions	1285:1319	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	5	44	theme	steric	1041:1046	arg1	repulsion					1048:1056	steric repulsion	1041:1056	steric repulsion that protects the nanoemulsion from oiling-off	1041:1103	An emulsion at pH 2 was unstable against creaming, and no phase separation occurred after 130 h; presumably, OSAS has a certain degree of steric repulsion that protects the nanoemulsion from oiling-off.
30267815	4	45	theme	stable	882:887	arg1	nanoemulsion					889:900	a more stable nanoemulsion	875:900	a more stable nanoemulsion	875:900	Adequate addition of OSAS (OSAS/phosphatidylcholine weight ratio = 3:7) led to the formation of a more stable nanoemulsion.
30267815	2	46	theme	anhydride-modified	373:390	arg1	starch					392:397	octenyl succinic anhydride-modified starch	356:397	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil	356:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	2	46	theme	anhydride-modified	373:390	arg1	OSAS					400:403	OSAS	400:403	OSAS	400:403	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	7	47	dep	microscopy	1344:1353	arg1	images					1355:1360	images	1355:1360	images	1355:1360	Transmission electron microscopy images showed that OSAS and phosphatidylcholine were both adsorbed around the surface of the emulsion.
30267815	1	48	theme	ionic	266:270	arg1	strength					272:279	moderate ionic strength	257:279	moderate ionic strength (>100 mmol/L NaCl)	257:298	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	48	theme	ionic	266:270	arg1	NaCl					294:297	>100 mmol/L NaCl	282:297	>100 mmol/L NaCl	282:297	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	6	49	dep	90 °C	1254:1258	arg1	to					1251:1252	to	1251:1252	to	1251:1252	The phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS were stable at high NaCl concentrations (≤800 mmol/L) and temperatures from 30 to 90 °C, which was attributed to steric and electrostatic repulsions.
30267815	3	50	theme	phosphatidylcholine-enhanced	703:730	arg1	nanoemulsions					732:744	phosphatidylcholine-enhanced nanoemulsions	703:744	phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	703:763	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	51	dep	variables	517:525	arg1	concentration					595:607	emulsifier concentration	584:607	emulsifier concentration	584:607	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	51	dep	variables	517:525	arg1	ratio					560:564	OSAS/phosphatidylcholine weight ratio	528:564	OSAS/phosphatidylcholine weight ratio	528:564	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	51	dep	variables	517:525	arg1	composition					571:581	oil composition	567:581	oil composition	567:581	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	51	dep	variables	517:525	arg1	etc.					610:613	etc.	610:613	etc.	610:613	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	2	52	theme	succinic	364:371	arg1	starch					392:397	octenyl succinic anhydride-modified starch	356:397	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil	356:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	2	52	theme	succinic	364:371	arg1	OSAS					400:403	OSAS	400:403	OSAS	400:403	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	3	53	theme	weight	553:558	arg1	concentration					595:607	emulsifier concentration	584:607	emulsifier concentration	584:607	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	53	theme	weight	553:558	arg1	ratio					560:564	OSAS/phosphatidylcholine weight ratio	528:564	OSAS/phosphatidylcholine weight ratio	528:564	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	53	theme	weight	553:558	arg1	composition					571:581	oil composition	567:581	oil composition	567:581	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	3	53	theme	weight	553:558	arg1	etc.					610:613	etc.	610:613	etc.	610:613	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	0	54	theme	anhydride-modified	93:110	arg1	starch					112:117	octenyl succinic anhydride-modified starch	76:117	octenyl succinic anhydride-modified starch	76:117	Improving the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch.
30267815	3	55	theme	variables	517:525	arg1	effect					507:512	The effect	503:512	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS	503:763	The effect of variables (OSAS/phosphatidylcholine weight ratio, oil composition, emulsifier concentration, etc.) and environmental stresses (pH, temperature, ionic strength, etc.) on the stability of phosphatidylcholine-enhanced nanoemulsions stabilized by OSAS was examined.
30267815	1	56	theme	low	246:248	arg1	pH					250:251	low pH	246:251	low pH	246:251	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	2	57	theme	nanoemulsions	417:429	arg1	stability					343:351	stability	343:351	stability	343:351	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	2	57	theme	nanoemulsions	417:429	arg1	formation					329:337	formation	329:337	formation	329:337	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	8	58	theme	adding	1484:1489	arg1	OSAS					1491:1494	adding OSAS	1484:1494	adding OSAS	1484:1494	These results showed that adding OSAS could improve the formation and stability of phosphatidylcholine-stabilized emulsions.
30267815	0	59	theme	succinic	84:91	arg1	starch					112:117	octenyl succinic anhydride-modified starch	76:117	octenyl succinic anhydride-modified starch	76:117	Improving the stability of phosphatidylcholine-enhanced nanoemulsions using octenyl succinic anhydride-modified starch.
30267815	7	60	theme	emulsion	1448:1455	arg1	surface					1433:1439	the surface	1429:1439	the surface of the emulsion	1429:1455	Transmission electron microscopy images showed that OSAS and phosphatidylcholine were both adsorbed around the surface of the emulsion.
30267815	2	61	theme	-stabilized	405:415	arg1	nanoemulsions					417:429	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions	356:429	octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil	356:500	This study investigated the formation and stability of octenyl succinic anhydride-modified starch (OSAS)-stabilized nanoemulsions enhanced with phosphatidylcholine for the encapsulation of soybean oil.
30267815	7	62	theme	Transmission	1322:1333	arg1	microscopy					1344:1353	Transmission electron microscopy images	1322:1360	Transmission electron microscopy images	1322:1360	Transmission electron microscopy images showed that OSAS and phosphatidylcholine were both adsorbed around the surface of the emulsion.
30267815	1	63	theme	>100 mmol/L	282:292	arg1	strength					272:279	moderate ionic strength	257:279	moderate ionic strength (>100 mmol/L NaCl)	257:298	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
30267815	1	63	theme	>100 mmol/L	282:292	arg1	NaCl					294:297	>100 mmol/L NaCl	282:297	>100 mmol/L NaCl	282:297	Phosphatidylcholine-coated emulsions are unstable and prone to phase separation under certain environmental stresses, such as low pH and moderate ionic strength (>100 mmol/L NaCl).
31261855	6	0	theme	MALDI-TOF	935:943	arg1	spectrometry					921:932	mass spectrometry	916:932	mass spectrometry (MALDI-TOF MS)	916:947	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	6	0	theme	MALDI-TOF	935:943	arg1	MS					945:946	MALDI-TOF MS	935:946	MALDI-TOF MS	935:946	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	2	1	theme	fucosyl-oligosaccharides	353:376	arg1	synthesis					340:348	the synthesis	336:348	the synthesis of fucosyl-oligosaccharides	336:376	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	7	2	theme	milk	1066:1069	arg1	oligosaccharides					1071:1086	human milk oligosaccharides	1060:1086	human milk oligosaccharides	1060:1086	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	4	3	theme	Enzyme	543:548	arg1	activity					550:557	Enzyme activity	543:557	Enzyme activity associated with the cell	543:582	Enzyme activity associated with the cell was recovered at 78% of its total activity.
31261855	5	4	theme	acceptor	764:771	arg1	pNP-Fuc					704:710	pNP-Fuc	704:710	pNP-Fuc	704:710	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	4	theme	acceptor	764:771	arg1	substrates					773:782	acceptor substrates	764:782	acceptor substrates	764:782	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	4	theme	acceptor	764:771	arg1	extract					692:698	α-l-fucosidase extract	677:698	α-l-fucosidase extract	677:698	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	7	5	contain	has	1024:1026	arg2	functions					1039:1047	biological functions	1028:1047	biological functions similar to human milk oligosaccharides	1028:1086	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	7	5	contain	has	1024:1026	arg1	fucosyl-oligosaccharide					974:996	the fucosyl-oligosaccharide	970:996	the fucosyl-oligosaccharide synthesized in this study	970:1022	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	6	6	theme	mass	916:919	arg1	spectrometry					921:932	mass spectrometry	916:932	mass spectrometry (MALDI-TOF MS)	916:947	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	6	6	theme	mass	916:919	arg1	MS					945:946	MALDI-TOF MS	935:946	MALDI-TOF MS	935:946	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	1	7	theme	probiotics	167:176	arg1	growth					157:162	the growth	153:162	the growth of probiotics in human gut	153:189	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	1	8	theme	innate	209:214	arg1	system					223:228	the innate immune system	205:228	the innate immune system	205:228	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	5	9	dep	25	808:809	arg1	to					805:806	to	805:806	to	805:806	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	1	10	theme	natural	121:127	arg1	Fucosyl-oligosaccharides					92:115	Fucosyl-oligosaccharides	92:115	Fucosyl-oligosaccharides	92:115	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	1	10	theme	natural	121:127	arg1	prebiotics					129:138	natural prebiotics	121:138	natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system	121:228	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	2	11	dep	Lactobacillus	277:289	arg1	GG					301:302	Lactobacillus rhamnosus GG	277:302	Lactobacillus rhamnosus GG	277:302	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	5	12	theme	α-l-fucosidase	677:690	arg1	pNP-Fuc					704:710	pNP-Fuc	704:710	pNP-Fuc	704:710	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	12	theme	α-l-fucosidase	677:690	arg1	substrates					773:782	acceptor substrates	764:782	acceptor substrates	764:782	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	12	theme	α-l-fucosidase	677:690	arg1	extract					692:698	α-l-fucosidase extract	677:698	α-l-fucosidase extract	677:698	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	1	13	theme	immune	216:221	arg1	system					223:228	the innate immune system	205:228	the innate immune system	205:228	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	1	14	from	growth	157:162	arg1	gut					187:189	human gut	181:189	human gut	181:189	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
31261855	6	15	with	product	855:861	arg1	D-lactose					868:876	D-lactose	868:876	D-lactose	868:876	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	0	16	theme	Fucosyl-Oligosaccharides	13:36	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Fucosyl-Oligosaccharides Using α-l-Fucosidase from Lactobacillus rhamnosus GG.	0:90	Synthesis of Fucosyl-Oligosaccharides Using α-l-Fucosidase from Lactobacillus rhamnosus GG.
31261855	5	17	dep	yield	796:800	arg1	%					810:810	%	810:810	%	810:810	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	0	18	dep	Lactobacillus	64:76	arg1	GG					88:89	Lactobacillus rhamnosus GG	64:89	Lactobacillus rhamnosus GG	64:89	Synthesis of Fucosyl-Oligosaccharides Using α-l-Fucosidase from Lactobacillus rhamnosus GG.
31261855	3	19	from	cells	466:470	arg1	release					449:455	its release	445:455	its release from the cells	445:470	Since α-lfucosidase is a membrane-bound enzyme, its release from the cells was induced by addition of 4-nitrophenyl-α-l-fucopyranoside (pNP-Fuc).
31261855	5	20	theme	donor	715:719	arg1	D-lactulose					749:759	D-lactulose	749:759	D-lactulose	749:759	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	20	theme	donor	715:719	arg1	substrate					721:729	donor substrate	715:729	donor substrate	715:729	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	5	20	theme	donor	715:719	arg1	D-lactose					736:744	D-lactose	736:744	D-lactose	736:744	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	7	21	theme	similar	1049:1055	arg1	functions					1039:1047	biological functions	1028:1047	biological functions similar to human milk oligosaccharides	1028:1086	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	3	22	theme	4-nitrophenyl-α-l-fucopyranoside	499:530	arg1	addition					487:494	addition	487:494	addition of 4-nitrophenyl-α-l-fucopyranoside (pNP-Fuc)	487:540	Since α-lfucosidase is a membrane-bound enzyme, its release from the cells was induced by addition of 4-nitrophenyl-α-l-fucopyranoside (pNP-Fuc).
31261855	3	23	theme	membrane-bound	422:435	arg1	α-lfucosidase					403:415	α-lfucosidase	403:415	α-lfucosidase	403:415	Since α-lfucosidase is a membrane-bound enzyme, its release from the cells was induced by addition of 4-nitrophenyl-α-l-fucopyranoside (pNP-Fuc).
31261855	3	23	theme	membrane-bound	422:435	arg1	enzyme					437:442	a membrane-bound enzyme	420:442	a membrane-bound enzyme	420:442	Since α-lfucosidase is a membrane-bound enzyme, its release from the cells was induced by addition of 4-nitrophenyl-α-l-fucopyranoside (pNP-Fuc).
31261855	7	24	theme	biological	1028:1037	arg1	functions					1039:1047	biological functions	1028:1047	biological functions similar to human milk oligosaccharides	1028:1086	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	4	25	theme	activity	618:625	arg1	activity					618:625	its total activity	608:625	its total activity	608:625	Enzyme activity associated with the cell was recovered at 78% of its total activity.
31261855	4	25	theme	activity	618:625	arg1	%					603:603	78%	601:603	78% of its total activity	601:625	Enzyme activity associated with the cell was recovered at 78% of its total activity.
31261855	7	26	theme	human	1060:1064	arg1	oligosaccharides					1071:1086	human milk oligosaccharides	1060:1086	human milk oligosaccharides	1060:1086	It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
31261855	2	27	theme	enzyme	325:330	arg1	use					313:315	the use	309:315	the use of this enzyme for the synthesis of fucosyl-oligosaccharides	309:376	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	2	27	theme	enzyme	325:330	arg1	release					249:255	the release	245:255	the release of α-lfucosidase by Lactobacillus rhamnosus GG	245:302	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	4	28	theme	total	612:616	arg1	activity					618:625	its total activity	608:625	its total activity	608:625	Enzyme activity associated with the cell was recovered at 78% of its total activity.
31261855	6	29	theme	reaction	846:853	arg1	Fucosyllactose					813:826	Fucosyllactose	813:826	Fucosyllactose	813:826	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	6	29	theme	reaction	846:853	arg1	product					855:861	a reaction product	844:861	a reaction product with D-lactose	844:876	Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS).
31261855	2	30	theme	α-lfucosidase	260:272	arg1	use					313:315	the use	309:315	the use of this enzyme for the synthesis of fucosyl-oligosaccharides	309:376	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	2	30	theme	α-lfucosidase	260:272	arg1	release					249:255	the release	245:255	the release of α-lfucosidase by Lactobacillus rhamnosus GG	245:302	In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated.
31261855	5	31	dep	%	810:810	arg1	25					808:809	25	808:809	25	808:809	Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and pNP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%.
31261855	1	32	theme	human	181:185	arg1	gut					187:189	human gut	181:189	human gut	181:189	Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system.
30600072	1	0	with	waste	164:168	arg1	content					182:188	a high content	175:188	a high content of carbohydrates	175:205	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	3	1	theme	carbon	500:505	arg1	source					507:512	carbon source	500:512	carbon source	500:512	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	0	2	theme	carbon	84:89	arg1	source					91:96	carbon source	84:96	carbon source	84:96	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	5	3	theme	cellulose	637:645	arg1	yield					647:651	The cellulose yield	633:651	The cellulose yield	633:651	The cellulose yield ranged around 2.816 ± 0.040 g/L for 28 days.
30600072	9	4	contain	have	1056:1059	arg1	BC					1047:1048	The BC	1043:1048	The BC	1043:1048	The BC could have potential use in chemical functionalization and in the preparation of biocomposites.
30600072	9	4	contain	have	1056:1059	arg2	use					1071:1073	potential use	1061:1073	potential use	1061:1073	The BC could have potential use in chemical functionalization and in the preparation of biocomposites.
30600072	8	5	theme	BC	964:965	arg1	production					950:959	The production	946:959	The production of BC with G. entanii using pecan nutshell as carbon source,	946:1020	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	8	5	theme	BC	964:965	arg1	report					1035:1040	the first report	1025:1040	the first report	1025:1040	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	8	6	theme	first	1029:1033	arg1	production					950:959	The production	946:959	The production of BC with G. entanii using pecan nutshell as carbon source,	946:1020	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	8	6	theme	first	1029:1033	arg1	report					1035:1040	the first report	1025:1040	the first report	1025:1040	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	2	7	theme	carbon	279:284	arg1	nutshell					253:260	pecan nutshell	247:260	pecan nutshell	247:260	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	2	7	theme	carbon	279:284	arg1	source					286:291	carbon source	279:291	carbon source	279:291	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	6	8	theme	morphological	702:714	arg1	similar					783:789	similar	783:789	similar	783:789	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	6	8	theme	morphological	702:714	arg1	properties					741:750	The morphological, structural and chemical properties	698:750	The morphological, structural and chemical properties of the cellulose produced	698:776	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	8	9	theme	pecan	989:993	arg1	nutshell					995:1002	pecan nutshell	989:1002	pecan nutshell	989:1002	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	7	10	theme	chemical	873:880	arg1	functionalization					882:898	the chemical functionalization	869:898	the chemical functionalization of BC	869:904	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	0	11	theme	chemical	106:113	arg1	functionalization					115:131	its chemical functionalization	102:131	its chemical functionalization	102:131	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	8	12	theme	carbon	1007:1012	arg1	source					1014:1019	carbon source	1007:1019	carbon source	1007:1019	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	8	13	with	production	950:959	arg1	G.					972:973	G.	972:973	G.	972:973	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	1	14	theme	high	177:180	arg1	content					182:188	a high content	175:188	a high content of carbohydrates	175:205	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	0	15	theme	cellulose	10:18	arg1	production					20:29	Bacterial cellulose production	0:29	Bacterial cellulose production by Gluconacetobacter entanii	0:58	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	2	16	theme	nanometric	381:390	arg1	characteristics					392:406	nanometric characteristics	381:406	nanometric characteristics	381:406	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	0	17	theme	Bacterial	0:8	arg1	production					20:29	Bacterial cellulose production	0:29	Bacterial cellulose production by Gluconacetobacter entanii	0:58	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	7	18	theme	crystallinity	931:943	arg1	reduction					914:922	the reduction	910:922	the reduction of its crystallinity	910:943	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	7	18	theme	crystallinity	931:943	arg1	functionalization					882:898	the chemical functionalization	869:898	the chemical functionalization of BC	869:904	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	9	19	theme	chemical	1078:1085	arg1	functionalization					1087:1103	chemical functionalization	1078:1103	chemical functionalization	1078:1103	The BC could have potential use in chemical functionalization and in the preparation of biocomposites.
30600072	2	20	theme	Gluconacetobacter	297:313	arg1	entanii					315:321	Gluconacetobacter entanii	297:321	Gluconacetobacter entanii	297:321	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	2	20	theme	Gluconacetobacter	297:313	arg1	bacterium					326:334	a bacterium	324:334	a bacterium that produces cellulose with high purity and nanometric characteristics	324:406	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	7	21	theme	spectroscopic	828:840	arg1	characterization					842:857	The spectroscopic characterization	824:857	The spectroscopic characterization	824:857	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	1	22	theme	carbohydrates	193:205	arg1	content					182:188	a high content	175:188	a high content of carbohydrates	175:205	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	4	23	theme	pecan	565:569	arg1	nutshell					571:578	the pecan nutshell	561:578	the pecan nutshell	561:578	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	4	23	theme	pecan	565:569	arg1	source					604:609	carbon source	597:609	carbon source	597:609	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	9	24	theme	potential	1061:1069	arg1	use					1071:1073	potential use	1061:1073	potential use	1061:1073	The BC could have potential use in chemical functionalization and in the preparation of biocomposites.
30600072	0	25	theme	Gluconacetobacter	34:50	arg1	entanii					52:58	Gluconacetobacter entanii	34:58	Gluconacetobacter entanii	34:58	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	6	26	theme	cellulose	759:767	arg1	similar					783:789	similar	783:789	similar	783:789	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	6	26	theme	cellulose	759:767	arg1	properties					741:750	The morphological, structural and chemical properties	698:750	The morphological, structural and chemical properties of the cellulose produced	698:776	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	3	27	theme	static	454:459	arg1	medium					469:474	a static culture medium	452:474	a static culture medium using pecan nutshell as carbon source and saccharose as control	452:538	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	0	28	theme	pecan	66:70	arg1	nutshell					72:79	pecan nutshell	66:79	pecan nutshell	66:79	Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization.
30600072	2	29	theme	chemical	225:232	arg1	composition					234:244	its chemical composition	221:244	its chemical composition	221:244	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	4	30	used	used	589:592	arg2	nutshell					571:578	the pecan nutshell	561:578	the pecan nutshell	561:578	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	4	30	used	used	589:592	arg2	source					604:609	carbon source	597:609	carbon source	597:609	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	1	31	theme	abundant	155:162	arg1	nutshell					140:147	Pecan nutshell	134:147	Pecan nutshell	134:147	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	1	31	theme	abundant	155:162	arg1	waste					164:168	an abundant waste	152:168	an abundant waste with a high content of carbohydrates	152:205	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	1	32	theme	Pecan	134:138	arg1	nutshell					140:147	Pecan nutshell	134:147	Pecan nutshell	134:147	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	1	32	theme	Pecan	134:138	arg1	waste					164:168	an abundant waste	152:168	an abundant waste with a high content of carbohydrates	152:205	Pecan nutshell is an abundant waste with a high content of carbohydrates.
30600072	8	33	dep	G.	972:973	arg1	entanii					975:981	G. entanii using pecan nutshell as carbon source,	972:1020	entanii	975:981	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	8	33	dep	G.	972:973	arg1	using					983:987	using	983:987	using pecan nutshell as carbon source	983:1019	The production of BC with G. entanii using pecan nutshell as carbon source, is the first report.
30600072	9	34	theme	biocomposites	1131:1143	arg1	preparation					1116:1126	the preparation	1112:1126	the preparation of biocomposites	1112:1143	The BC could have potential use in chemical functionalization and in the preparation of biocomposites.
30600072	7	35	theme	BC	903:904	arg1	reduction					914:922	the reduction	910:922	the reduction of its crystallinity	910:943	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	7	35	theme	BC	903:904	arg1	functionalization					882:898	the chemical functionalization	869:898	the chemical functionalization of BC	869:904	The spectroscopic characterization indicated the chemical functionalization of BC and the reduction of its crystallinity.
30600072	4	36	theme	carbon	597:602	arg1	nutshell					571:578	the pecan nutshell	561:578	the pecan nutshell	561:578	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	4	36	theme	carbon	597:602	arg1	source					604:609	carbon source	597:609	carbon source	597:609	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	6	37	theme	chemical	732:739	arg1	similar					783:789	similar	783:789	similar	783:789	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	6	37	theme	chemical	732:739	arg1	properties					741:750	The morphological, structural and chemical properties	698:750	The morphological, structural and chemical properties of the cellulose produced	698:776	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	3	38	theme	pecan	482:486	arg1	nutshell					488:495	pecan nutshell	482:495	pecan nutshell	482:495	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	4	39	theme	BC	629:630	arg1	production					615:624	production	615:624	production of BC	615:630	Results showed that the pecan nutshell could be used as carbon source for production of BC.
30600072	3	40	theme	culture	461:467	arg1	medium					469:474	a static culture medium	452:474	a static culture medium using pecan nutshell as carbon source and saccharose as control	452:538	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	3	41	theme	Bacterial	409:417	arg1	cellulose					419:427	Bacterial cellulose	409:427	Bacterial cellulose (BC)	409:432	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	3	41	theme	Bacterial	409:417	arg1	BC					430:431	BC	430:431	BC	430:431	Bacterial cellulose (BC) was obtained from a static culture medium using pecan nutshell as carbon source and saccharose as control.
30600072	2	42	theme	pecan	247:251	arg1	nutshell					253:260	pecan nutshell	247:260	pecan nutshell	247:260	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	2	42	theme	pecan	247:251	arg1	source					286:291	carbon source	279:291	carbon source	279:291	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	6	43	theme	structural	717:726	arg1	similar					783:789	similar	783:789	similar	783:789	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	6	43	theme	structural	717:726	arg1	properties					741:750	The morphological, structural and chemical properties	698:750	The morphological, structural and chemical properties of the cellulose produced	698:776	The morphological, structural and chemical properties of the cellulose produced were similar to those reported for others BC.
30600072	2	44	theme	high	365:368	arg1	purity					370:375	high purity	365:375	high purity	365:375	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	2	45	used	used	271:274	arg2	nutshell					253:260	pecan nutshell	247:260	pecan nutshell	247:260	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30600072	2	45	used	used	271:274	arg2	source					286:291	carbon source	279:291	carbon source	279:291	According to its chemical composition, pecan nutshell could be used as carbon source for Gluconacetobacter entanii, a bacterium that produces cellulose with high purity and nanometric characteristics.
30052146	4	0	contain	contained	1005:1013	arg1	RESULTS					968:974	RESULTS	968:974	RESULTS The bagasse and residue oils	968:1003	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	0	contain	contained	1005:1013	arg2	%					1050:1050	1.13%-2.38%	1040:1050	1.13%-2.38%	1040:1050	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	0	contain	contained	1005:1013	arg2	1.89-9.20 mg/KOH/g					1070:1087	1.89-9.20 mg/KOH/g	1070:1087	1.89-9.20 mg/KOH/g	1070:1087	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	0	contain	contained	1005:1013	arg2	contents					1030:1037	lower moisture contents	1015:1037	lower moisture contents (1.13%-2.38%)	1015:1051	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	0	contain	contained	1005:1013	arg2	values					1062:1067	acid values	1057:1067	acid values (1.89-9.20 mg/KOH/g)	1057:1088	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	1	theme	oil	1173:1175	arg1	yield					1177:1181	oil yield	1173:1181	oil yield	1173:1181	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	11	2	theme	non-food	2081:2088	arg1	purposes					2101:2108	non-food industrial purposes	2081:2108	non-food industrial purposes	2081:2108	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	1	3	theme	radical-scavenging	233:250	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	10	4	theme	scavenging	1891:1900	arg1	potentials					1902:1911	the scavenging potentials	1887:1911	the scavenging potentials of the standards used	1887:1933	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	3	5	theme	gas	825:827	arg1	detection					867:875	gas chromatography-fluorescent ionization detection	825:875	gas chromatography-fluorescent ionization detection	825:875	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	1	6	dep	residues	362:369	arg1	GH					423:424	GH	423:424	GH	423:424	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	CC					383:384	CC	383:384	CC	383:384	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	leaves					470:475	leaves	470:475	leaves	470:475	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	leaves					498:503	leaves	498:503	leaves	498:503	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	hulls					416:420	peanut hulls	409:420	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	plants					438:443	medicinal plants	428:443	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	cobs					377:380	corn cobs	372:380	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	chaff					397:401	tigernut chaff	388:401	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	Sphenocentrum					446:458	Sphenocentrum	446:458	Sphenocentrum	446:458	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	6	dep	residues	362:369	arg1	TB					404:405	TB	404:405	TB	404:405	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	9	7	theme	oxalate	1787:1793	arg1	amounts					1750:1756	the highest amounts	1738:1756	the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml)	1738:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	7	theme	oxalate	1787:1793	arg1	39.47 µg/ml					1796:1806	39.47 µg/ml	1796:1806	39.47 µg/ml	1796:1806	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	7	theme	oxalate	1787:1793	arg1	53.81 µg/ml					1770:1780	53.81 µg/ml	1770:1780	53.81 µg/ml	1770:1780	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	7	theme	oxalate	1787:1793	arg1	oxalate					1787:1793	oxalate	1787:1793	oxalate (39.47 µg/ml)	1787:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	7	theme	oxalate	1787:1793	arg1	phytate					1761:1767	phytate	1761:1767	phytate (53.81 µg/ml)	1761:1781	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	5	8	theme	73.65	1243:1247	arg1	%					1248:1248	%	1248:1248	%	1248:1248	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	4	9	theme	bagasse	980:986	arg1	oils					1000:1003	The bagasse and residue oils	976:1003	RESULTS The bagasse and residue oils	968:1003	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	10	theme	saponification	1147:1160	arg1	value					1162:1166	saponification value	1147:1166	saponification value	1147:1166	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	1	11	from	bagasses	280:287	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	11	from	bagasses	280:287	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	11	from	bagasses	280:287	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	11	from	bagasses	280:287	arg1	oils					266:269	oils	266:269	oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	266:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	11	from	bagasses	280:287	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	12	theme	tigernut	388:395	arg1	chaff					397:401	tigernut chaff	388:401	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	12	theme	tigernut	388:395	arg1	TB					404:405	TB	404:405	TB	404:405	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	10	13	theme	standards	1920:1928	arg1	potentials					1902:1911	the scavenging potentials	1887:1911	the scavenging potentials of the standards used	1887:1933	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	4	14	theme	residue	992:998	arg1	oils					1000:1003	The bagasse and residue oils	976:1003	RESULTS The bagasse and residue oils	968:1003	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	11	15	theme	acids	2009:2013	arg1	stability					1967:1975	the thermal stability	1955:1975	the thermal stability	1955:1975	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	amount					1981:1986	amount	1981:1986	amount	1981:1986	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	SFAs					2016:2019	SFAs	2016:2019	SFAs	2016:2019	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	CA					2033:2034	CA	2033:2034	CA	2033:2034	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	15	theme	acids	2009:2013	arg1	CC					2037:2038	CC	2037:2038	CC	2037:2038	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	3	16	theme	ionization	856:865	arg1	detection					867:875	gas chromatography-fluorescent ionization detection	825:875	gas chromatography-fluorescent ionization detection	825:875	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	0	17	from	Residues	72:79	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	0	17	from	Residues	72:79	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	6	18	contain	contained	1342:1350	arg2	%					1378:1378	19.65%	1373:1378	19.65%	1373:1378	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	6	18	contain	contained	1342:1350	arg2	acid					1367:1370	behenic acid	1359:1370	behenic acid (19.65%)	1359:1379	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	6	18	contain	contained	1342:1350	arg1	oil					1338:1340	CC oil	1335:1340	CC oil	1335:1340	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	8	19	from	oil	1644:1646	arg1	abundant					1629:1636	abundant	1629:1636	abundant	1629:1636	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	11	20	theme	oil	2132:2134	arg1	properties					2136:2145	CS oil properties	2129:2145	CS oil properties	2129:2145	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	4	21	theme	%	1044:1044	arg1	%					1050:1050	1.13%-2.38%	1040:1050	1.13%-2.38%	1040:1050	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	21	theme	%	1044:1044	arg1	contents					1030:1037	lower moisture contents	1015:1037	lower moisture contents (1.13%-2.38%)	1015:1051	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	11	22	theme	oxidative	2216:2224	arg1	stress					2226:2231	oxidative stress	2216:2231	oxidative stress	2216:2231	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	1	23	theme	corn	372:375	arg1	CC					383:384	CC	383:384	CC	383:384	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	23	theme	corn	372:375	arg1	cobs					377:380	corn cobs	372:380	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	6	24	theme	%	1407:1407	arg1	FA					1419:1420	87.04% saturated FA	1402:1420	87.04% saturated FA	1402:1420	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	10	25	from	concentrations	1863:1876	arg1	oil					1813:1815	TB oil	1810:1815	TB oil	1810:1815	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	10	25	from	concentrations	1863:1876	arg1	oils					1821:1824	oils	1821:1824	oils	1821:1824	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	2	26	theme	isopropyl	681:689	arg1	3:2					700:702	3:2	700:702	3:2	700:702	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	2	26	theme	isopropyl	681:689	arg1	alcohol					691:697	isopropyl alcohol	681:697	isopropyl alcohol (3:2)	681:703	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	1	27	from	properties	164:173	arg1	PM					568:569	PM	568:569	PM	568:569	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	CG					600:601	CG	600:601	CG	600:601	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	bagasses					280:287	the bagasses	276:287	the bagasses	276:287	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	CA					308:309	Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	290:543	CA	308:309	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	stalks					592:597	Chloris gayana stalks	577:597	Chloris gayana stalks (CG)	577:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	maximum					559:565	Panicum maximum	551:565	Panicum maximum (PM)	551:570	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	27	from	properties	164:173	arg1	PP					546:547	PP	546:547	PP	546:547	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	4	28	theme	moisture	1021:1028	arg1	%					1050:1050	1.13%-2.38%	1040:1050	1.13%-2.38%	1040:1050	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	28	theme	moisture	1021:1028	arg1	contents					1030:1037	lower moisture contents	1015:1037	lower moisture contents (1.13%-2.38%)	1015:1051	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	1	29	theme	Chloris	577:583	arg1	CG					600:601	CG	600:601	CG	600:601	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	29	theme	Chloris	577:583	arg1	stalks					592:597	Chloris gayana stalks	577:597	Chloris gayana stalks (CG)	577:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	6	30	theme	saturated	1409:1417	arg1	FA					1419:1420	87.04% saturated FA	1402:1420	87.04% saturated FA	1402:1420	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	6	31	theme	87.04	1402:1406	arg1	%					1407:1407	%	1407:1407	%	1407:1407	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	1	32	theme	physicochemical	148:162	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	32	theme	physicochemical	148:162	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	32	theme	physicochemical	148:162	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	33	from	stalks	592:597	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	33	from	stalks	592:597	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	33	from	stalks	592:597	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	33	from	stalks	592:597	arg1	oils					266:269	oils	266:269	oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	266:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	33	from	stalks	592:597	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	11	34	theme	GH	2045:2046	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	34	theme	GH	2045:2046	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	5	35	theme	lignoceric	1283:1292	arg1	acid					1329:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	11	36	theme	fatty	2003:2007	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	36	theme	fatty	2003:2007	arg1	SFAs					2016:2019	SFAs	2016:2019	SFAs	2016:2019	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	36	theme	fatty	2003:2007	arg1	CC					2037:2038	CC	2037:2038	CC	2037:2038	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	36	theme	fatty	2003:2007	arg1	CA					2033:2034	CA	2033:2034	CA	2033:2034	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	36	theme	fatty	2003:2007	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	1	37	dep	OBJECTIVE	113:121	arg1	evaluated					134:142	evaluated	134:142	evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	134:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	0	38	theme	Medicinal	82:90	arg1	Plants					92:97	Medicinal Plants	82:97	Medicinal Plants	82:97	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	1	39	theme	Saccharum	316:324	arg1	SB					344:345	Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders	316:521	SB	344:345	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	3	40	theme	free	882:885	arg1	potentials					906:915	free radical-scavenging potentials	882:915	free radical-scavenging potentials using spectrophotometric methods of determination	882:965	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	6	41	theme	unsaturated	1449:1459	arg1	FA					1461:1462	56% unsaturated FA	1445:1462	56% unsaturated FA	1445:1462	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	5	42	theme	saturated	1250:1258	arg1	FA					1260:1261	73.65% saturated FA	1243:1261	73.65% saturated FA	1243:1261	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	11	43	theme	short	1991:1995	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	43	theme	short	1991:1995	arg1	SFAs					2016:2019	SFAs	2016:2019	SFAs	2016:2019	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	43	theme	short	1991:1995	arg1	CC					2037:2038	CC	2037:2038	CC	2037:2038	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	43	theme	short	1991:1995	arg1	CA					2033:2034	CA	2033:2034	CA	2033:2034	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	43	theme	short	1991:1995	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	1	44	theme	stem	338:341	arg1	SB					344:345	Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders	316:521	SB	344:345	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	8	45	theme	SJ	1714:1715	arg1	oil					1717:1719	SJ oil	1714:1719	SJ oil	1714:1719	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	6	46	theme	56	1445:1446	arg1	%					1447:1447	%	1447:1447	%	1447:1447	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	5	47	contain	contained	1233:1241	arg2	FA					1260:1261	73.65% saturated FA	1243:1261	73.65% saturated FA	1243:1261	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	47	contain	contained	1233:1241	arg1	oils					1228:1231	SB oils	1225:1231	SB oils	1225:1231	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	47	contain	contained	1233:1241	arg2	abundance					1270:1278	an abundance	1267:1278	an abundance of lignoceric, palmitoleic, oleic, and arachidic acid	1267:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	48	theme	acid	1329:1332	arg1	FA					1260:1261	73.65% saturated FA	1243:1261	73.65% saturated FA	1243:1261	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	48	theme	acid	1329:1332	arg1	abundance					1270:1278	an abundance	1267:1278	an abundance of lignoceric, palmitoleic, oleic, and arachidic acid	1267:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	49	theme	oils	266:269	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	49	theme	oils	266:269	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	49	theme	oils	266:269	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	49	theme	oils	266:269	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	3	50	theme	spectrophotometric	923:940	arg1	methods					942:948	spectrophotometric methods	923:948	spectrophotometric methods of determination	923:965	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	10	51	theme	higher	1856:1861	arg1	concentrations					1863:1876	higher concentrations	1856:1876	higher concentrations	1856:1876	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	0	52	theme	Antioxidant	13:23	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	11	53	theme	therapeutic	2169:2179	arg1	purposes					2181:2188	therapeutic purposes	2169:2188	therapeutic purposes	2169:2188	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	1	54	theme	gayana	585:590	arg1	CG					600:601	CG	600:601	CG	600:601	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	54	theme	gayana	585:590	arg1	stalks					592:597	Chloris gayana stalks	577:597	Chloris gayana stalks (CG)	577:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	5	55	theme	palmitoleic	1295:1305	arg1	acid					1329:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	56	theme	78	1201:1202	arg1	%					1203:1203	%	1203:1203	%	1203:1203	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	57	from	CA	308:309	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	57	from	CA	308:309	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	57	from	CA	308:309	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	57	from	CA	308:309	arg1	oils					266:269	oils	266:269	oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	266:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	57	from	CA	308:309	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	58	theme	afer	297:300	arg1	CA					308:309	Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	290:543	CA	308:309	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	58	theme	afer	297:300	arg1	PP					546:547	PP	546:547	PP	546:547	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	8	59	theme	phytochemical	1554:1566	arg1	composition					1568:1578	The phytochemical composition	1550:1578	The phytochemical composition	1550:1578	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	3	60	theme	physicochemical	733:747	arg1	properties					749:758	physicochemical properties	733:758	physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination	733:965	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	7	61	from	CS	1479:1480	arg1	Oils					1465:1468	Oils	1465:1468	Oils from SJ, CS, PP, PM, and CG	1465:1496	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	5	62	theme	oleic	1308:1312	arg1	acid					1329:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	63	theme	saturated	1205:1213	arg1	FA					1215:1216	78% saturated FA	1201:1216	78% saturated FA	1201:1216	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	64	theme	phytochemical	196:208	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	64	theme	phytochemical	196:208	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	7	65	from	PP	1483:1484	arg1	Oils					1465:1468	Oils	1465:1468	Oils from SJ, CS, PP, PM, and CG	1465:1496	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	11	66	theme	CS	2129:2130	arg1	properties					2136:2145	CS oil properties	2129:2145	CS oil properties	2129:2145	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	9	67	theme	phytate	1761:1767	arg1	amounts					1750:1756	the highest amounts	1738:1756	the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml)	1738:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	67	theme	phytate	1761:1767	arg1	39.47 µg/ml					1796:1806	39.47 µg/ml	1796:1806	39.47 µg/ml	1796:1806	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	67	theme	phytate	1761:1767	arg1	53.81 µg/ml					1770:1780	53.81 µg/ml	1770:1780	53.81 µg/ml	1770:1780	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	67	theme	phytate	1761:1767	arg1	oxalate					1787:1793	oxalate	1787:1793	oxalate (39.47 µg/ml)	1787:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	67	theme	phytate	1761:1767	arg1	phytate					1761:1767	phytate	1761:1767	phytate (53.81 µg/ml)	1761:1781	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	7	68	from	PM	1487:1488	arg1	Oils					1465:1468	Oils	1465:1468	Oils from SJ, CS, PP, PM, and CG	1465:1496	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	0	69	from	Fodders	104:110	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	0	69	from	Fodders	104:110	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	1	70	theme	Panicum	551:557	arg1	PM					568:569	PM	568:569	PM	568:569	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	70	theme	Panicum	551:557	arg1	maximum					559:565	Panicum maximum	551:565	Panicum maximum (PM)	551:570	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	71	theme	free	228:231	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	11	72	theme	thermal	1959:1965	arg1	stability					1967:1975	the thermal stability	1955:1975	the thermal stability	1955:1975	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	2	73	theme	alcohol	691:697	arg1	mixture					657:663	a mixture	655:663	a mixture of n-hexane and isopropyl alcohol (3:2)	655:703	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	1	74	dep	Sphenocentrum	446:458	arg1	jollyanum					460:468	Sphenocentrum jollyanum	446:468	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	3	75	theme	FAs	806:808	arg1	contents					810:817	FAs contents	806:817	FAs contents	806:817	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	7	76	dep	%	1521:1521	arg1	FA					1546:1547	unsaturated FA	1534:1547	unsaturated FA	1534:1547	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	4	77	theme	refractive	1127:1136	arg1	indices					1138:1144	the least refractive indices	1117:1144	the least refractive indices	1117:1144	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	5	78	theme	%	1248:1248	arg1	FA					1260:1261	73.65% saturated FA	1243:1261	73.65% saturated FA	1243:1261	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	79	theme	fatty	176:180	arg1	FA					188:189	FA	188:189	FA	188:189	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	79	theme	fatty	176:180	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	79	theme	fatty	176:180	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	3	80	theme	chromatography-fluorescent	829:854	arg1	detection					867:875	gas chromatography-fluorescent ionization detection	825:875	gas chromatography-fluorescent ionization detection	825:875	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	1	81	from	potentials	252:261	arg1	PM					568:569	PM	568:569	PM	568:569	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	CG					600:601	CG	600:601	CG	600:601	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	bagasses					280:287	the bagasses	276:287	the bagasses	276:287	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	CA					308:309	Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	290:543	CA	308:309	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	stalks					592:597	Chloris gayana stalks	577:597	Chloris gayana stalks (CG)	577:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	maximum					559:565	Panicum maximum	551:565	Panicum maximum (PM)	551:570	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	81	from	potentials	252:261	arg1	PP					546:547	PP	546:547	PP	546:547	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	5	82	theme	SB	1225:1226	arg1	oils					1228:1231	SB oils	1225:1231	SB oils	1225:1231	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	83	theme	peanut	409:414	arg1	GH					423:424	GH	423:424	GH	423:424	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	83	theme	peanut	409:414	arg1	hulls					416:420	peanut hulls	409:420	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	11	84	theme	industrial	2090:2099	arg1	purposes					2101:2108	non-food industrial purposes	2081:2108	non-food industrial purposes	2081:2108	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	7	85	from	SJ	1475:1476	arg1	Oils					1465:1468	Oils	1465:1468	Oils from SJ, CS, PP, PM, and CG	1465:1496	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	8	86	from	abundant	1629:1636	arg1	oil					1644:1646	PM oil	1641:1646	PM oil	1641:1646	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	6	87	theme	TB	1429:1430	arg1	oil					1432:1434	TB oil	1429:1434	TB oil	1429:1434	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	11	88	theme	SB	2029:2030	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	88	theme	SB	2029:2030	arg1	SFAs					2016:2019	SFAs	2016:2019	SFAs	2016:2019	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	88	theme	SB	2029:2030	arg1	CC					2037:2038	CC	2037:2038	CC	2037:2038	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	88	theme	SB	2029:2030	arg1	CA					2033:2034	CA	2033:2034	CA	2033:2034	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	88	theme	SB	2029:2030	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	10	89	from	SJ	1831:1832	arg1	oil					1813:1815	TB oil	1810:1815	TB oil	1810:1815	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	10	89	from	SJ	1831:1832	arg1	oils					1821:1824	oils	1821:1824	oils	1821:1824	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	1	90	theme	agricultural	349:360	arg1	CS					506:507	CS	506:507	CS	506:507	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	90	theme	agricultural	349:360	arg1	residues					362:369	agricultural residues	349:369	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	90	theme	agricultural	349:360	arg1	SJ					478:479	SJ	478:479	SJ	478:479	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	4	91	dep	RESULTS	968:974	arg1	oils					1000:1003	The bagasse and residue oils	976:1003	RESULTS The bagasse and residue oils	968:1003	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	10	92	theme	TB	1810:1811	arg1	oil					1813:1815	TB oil	1810:1815	TB oil	1810:1815	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	10	93	from	CS	1838:1839	arg1	oil					1813:1815	TB oil	1810:1815	TB oil	1810:1815	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	10	93	from	CS	1838:1839	arg1	oils					1821:1824	oils	1821:1824	oils	1821:1824	TB oil and oils from SJ and CS, especially at higher concentrations, matched the scavenging potentials of the standards used.
30052146	8	94	theme	PM	1641:1642	arg1	oil					1644:1646	PM oil	1641:1646	PM oil	1641:1646	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	6	95	theme	behenic	1359:1365	arg1	acid					1367:1370	behenic acid	1359:1370	behenic acid (19.65%)	1359:1379	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	6	95	theme	behenic	1359:1365	arg1	%					1378:1378	19.65%	1373:1378	19.65%	1373:1378	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	0	96	from	Plants	92:97	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	0	96	from	Plants	92:97	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	4	97	theme	lower	1015:1019	arg1	%					1050:1050	1.13%-2.38%	1040:1050	1.13%-2.38%	1040:1050	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	97	theme	lower	1015:1019	arg1	contents					1030:1037	lower moisture contents	1015:1037	lower moisture contents (1.13%-2.38%)	1015:1051	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	11	98	theme	stress	2226:2231	arg1	relief					2206:2211	relief	2206:2211	relief of oxidative stress	2206:2231	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	0	99	theme	Agricultural	59:70	arg1	Residues					72:79	Agricultural Residues	59:79	Agricultural Residues	59:79	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	8	100	from	abundant	1702:1709	arg1	oil					1717:1719	SJ oil	1714:1719	SJ oil	1714:1719	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	3	101	theme	radical-scavenging	887:904	arg1	potentials					906:915	free radical-scavenging potentials	882:915	free radical-scavenging potentials using spectrophotometric methods of determination	882:965	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	6	102	theme	CC	1335:1336	arg1	oil					1338:1340	CC oil	1335:1340	CC oil	1335:1340	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	11	103	theme	chain	1997:2001	arg1	oils					2048:2051	GH oils	2045:2051	GH oils	2045:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	103	theme	chain	1997:2001	arg1	SFAs					2016:2019	SFAs	2016:2019	SFAs	2016:2019	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	103	theme	chain	1997:2001	arg1	CC					2037:2038	CC	2037:2038	CC	2037:2038	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	103	theme	chain	1997:2001	arg1	CA					2033:2034	CA	2033:2034	CA	2033:2034	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	11	103	theme	chain	1997:2001	arg1	acids					2009:2013	short chain fatty acids	1991:2013	short chain fatty acids (SFAs) of the SB	1991:2030	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	0	104	from	Bagasses	49:56	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	0	104	from	Bagasses	49:56	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	1	105	theme	medicinal	428:436	arg1	plants					438:443	medicinal plants	428:443	agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ)	349:480	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	106	theme	officinarum	326:336	arg1	SB					344:345	Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders	316:521	SB	344:345	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	6	107	theme	GH	1386:1387	arg1	oil					1389:1391	GH oil	1386:1391	GH oil	1386:1391	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	7	108	from	CG	1495:1496	arg1	Oils					1465:1468	Oils	1465:1468	Oils from SJ, CS, PP, PM, and CG	1465:1496	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	6	109	theme	%	1447:1447	arg1	FA					1461:1462	56% unsaturated FA	1445:1462	56% unsaturated FA	1445:1462	CC oil contained mostly behenic acid (19.65%), and GH oil produced 87.04% saturated FA, while TB oil produced 56% unsaturated FA.
30052146	8	110	from	oil	1717:1719	arg1	abundant					1702:1709	abundant	1702:1709	abundant	1702:1709	The phytochemical composition showed that ribalinidine and sapogenin were most abundant in PM oil, while lunamarine, kaempferol, and catechin were most abundant in SJ oil.
30052146	4	111	theme	acid	1057:1060	arg1	1.89-9.20 mg/KOH/g					1070:1087	1.89-9.20 mg/KOH/g	1070:1087	1.89-9.20 mg/KOH/g	1070:1087	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	4	111	theme	acid	1057:1060	arg1	values					1062:1067	acid values	1057:1067	acid values (1.89-9.20 mg/KOH/g)	1057:1088	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	2	112	from	samples	626:632	arg1	Oils					612:615	METHOD Oils	605:615	METHOD Oils from the samples	605:632	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	5	113	theme	arachidic	1319:1327	arg1	acid					1329:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	lignoceric, palmitoleic, oleic, and arachidic acid	1283:1332	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	9	114	theme	GH	1722:1723	arg1	oil					1725:1727	GH oil	1722:1727	GH oil	1722:1727	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	0	115	theme	Oils	39:42	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	0	115	theme	Oils	39:42	arg1	Properties					25:34	Antioxidant Properties	13:34	Antioxidant Properties	13:34	Nutrient and Antioxidant Properties of Oils from Bagasses, Agricultural Residues, Medicinal Plants, and Fodders.
30052146	3	116	theme	determination	953:965	arg1	methods					942:948	spectrophotometric methods	923:948	spectrophotometric methods of determination	923:965	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	11	117	theme	Due	1948:1950	arg1	CONCLUSIONS					1936:1946	CONCLUSIONS	1936:1946	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils	1936:2051	CONCLUSIONS Due to the thermal stability and amount of short chain fatty acids (SFAs) of the SB, CA, CC, and GH oils, they are more suitable for non-food industrial purposes, while TB, SJ, and CS oil properties are recommendable for therapeutic purposes, especially for relief of oxidative stress.
30052146	4	118	theme	least	1121:1125	arg1	indices					1138:1144	the least refractive indices	1117:1144	the least refractive indices	1117:1144	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	1	119	theme	Costus	290:295	arg1	CA					308:309	Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	290:543	CA	308:309	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	119	theme	Costus	290:295	arg1	PP					546:547	PP	546:547	PP	546:547	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	3	120	theme	standard	766:773	arg1	procedures					775:784	standard procedures	766:784	standard procedures	766:784	The oils were analyzed for physicochemical properties using standard procedures, phytochemicals and FAs contents using gas chromatography-fluorescent ionization detection, and free radical-scavenging potentials using spectrophotometric methods of determination.
30052146	9	121	theme	highest	1742:1748	arg1	amounts					1750:1756	the highest amounts	1738:1756	the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml)	1738:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	121	theme	highest	1742:1748	arg1	39.47 µg/ml					1796:1806	39.47 µg/ml	1796:1806	39.47 µg/ml	1796:1806	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	121	theme	highest	1742:1748	arg1	53.81 µg/ml					1770:1780	53.81 µg/ml	1770:1780	53.81 µg/ml	1770:1780	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	121	theme	highest	1742:1748	arg1	oxalate					1787:1793	oxalate	1787:1793	oxalate (39.47 µg/ml)	1787:1807	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	9	121	theme	highest	1742:1748	arg1	phytate					1761:1767	phytate	1761:1767	phytate (53.81 µg/ml)	1761:1781	GH oil produced the highest amounts of phytate (53.81 µg/ml) and oxalate (39.47 µg/ml).
30052146	5	122	theme	%	1203:1203	arg1	FA					1215:1216	78% saturated FA	1201:1216	78% saturated FA	1201:1216	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	1	123	theme	stem	302:305	arg1	CA					308:309	Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	290:543	CA	308:309	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	123	theme	stem	302:305	arg1	PP					546:547	PP	546:547	PP	546:547	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	7	124	theme	unsaturated	1534:1544	arg1	FA					1546:1547	unsaturated FA	1534:1547	unsaturated FA	1534:1547	Oils from SJ, CS, PP, PM, and CG contained between 48.34% and 57.09% unsaturated FA.
30052146	1	125	from	maximum	559:565	arg1	properties					164:173	the physicochemical properties	144:173	the physicochemical properties	144:173	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	125	from	maximum	559:565	arg1	acid					182:185	fatty acid	176:185	fatty acid (FA)	176:190	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	125	from	maximum	559:565	arg1	potentials					252:261	free radical-scavenging potentials	228:261	free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	228:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	125	from	maximum	559:565	arg1	oils					266:269	oils	266:269	oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG)	266:602	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	1	125	from	maximum	559:565	arg1	compositions					210:221	phytochemical compositions	196:221	phytochemical compositions	196:221	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	2	126	theme	n-hexane	668:675	arg1	mixture					657:663	a mixture	655:663	a mixture of n-hexane and isopropyl alcohol (3:2)	655:703	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	4	127	theme	GH	1101:1102	arg1	oil					1104:1106	the GH oil	1097:1106	the GH oil	1097:1106	RESULTS The bagasse and residue oils contained lower moisture contents (1.13%-2.38%) and acid values (1.89-9.20 mg/KOH/g), while the GH oil produced the least refractive indices, saponification value, and oil yield.
30052146	5	128	contain	contained	1191:1199	arg2	FA					1215:1216	78% saturated FA	1201:1216	78% saturated FA	1201:1216	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	5	128	contain	contained	1191:1199	arg1	oil					1187:1189	CA oil	1184:1189	CA oil	1184:1189	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30052146	2	129	theme	METHOD	605:610	arg1	Oils					612:615	METHOD Oils	605:615	METHOD Oils from the samples	605:632	METHOD Oils from the samples were extracted using a mixture of n-hexane and isopropyl alcohol (3:2).
30052146	1	130	dep	SB	344:345	arg1	purpureum					535:543	Pennisetum purpureum	524:543	Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum	316:543	OBJECTIVE This study evaluated the physicochemical properties, fatty acid (FA) and phytochemical compositions, and free radical-scavenging potentials of oils from the bagasses: Costus afer stem (CA) and Saccharum officinarum stem (SB); agricultural residues; corn cobs (CC); tigernut chaff (TB); peanut hulls (GH); medicinal plants: Sphenocentrum jollyanum leaves (SJ) and Senna alata leaves (CS); and fodders: Pennisetum purpureum (PP), Panicum maximum (PM), and Chloris gayana stalks (CG).
30052146	5	131	theme	CA	1184:1185	arg1	oil					1187:1189	CA oil	1184:1189	CA oil	1184:1189	CA oil contained 78% saturated FA, while SB oils contained 73.65% saturated FA and an abundance of lignoceric, palmitoleic, oleic, and arachidic acid.
30312748	7	0	theme	higher	1377:1382	arg1	concentration					1398:1410	higher total polymer concentration	1377:1410	higher total polymer concentration	1377:1410	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	4	1	theme	molecular	821:829	arg1	weight					831:836	low molecular weight	817:836	low molecular weight chitosan (CS) in different weight ratios in water	817:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	10	2	theme	polymer	1841:1847	arg1	matrix					1849:1854	the polymer matrix	1837:1854	the polymer matrix of the BLK wafers	1837:1872	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	11	3	theme	swelling	2079:2086	arg1	capacity					2088:2095	higher swelling capacity	2072:2095	higher swelling capacity	2072:2095	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	10	4	with	matrix	1924:1929	arg1	aspirin					1936:1942	aspirin	1936:1942	aspirin dispersed over the surface	1936:1969	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	3	5	theme	wafers	550:555	arg1	optimization					524:535	optimization	524:535	optimization	524:535	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	3	5	theme	wafers	550:555	arg1	characterization					503:518	characterization	503:518	characterization	503:518	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	3	5	theme	wafers	550:555	arg1	development					490:500	development	490:500	development	490:500	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	7	6	with	Wafers	1365:1370	arg1	concentration					1398:1410	higher total polymer concentration	1377:1410	higher total polymer concentration	1377:1410	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	10	7	theme	BLK	1863:1865	arg1	wafers					1867:1872	the BLK wafers	1859:1872	the BLK wafers	1859:1872	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	1	8	theme	demographic	169:179	arg1	ageing					181:186	demographic ageing	169:186	demographic ageing	169:186	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	13	9	theme	sample	2532:2537	arg1	3:1					2527:2529	CAR 3:1	2523:2529	CAR 3:1 (sample DL1)	2523:2542	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	9	theme	sample	2532:2537	arg1	DL1					2539:2541	sample DL1	2532:2541	sample DL1	2532:2541	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	4	10	theme	Blank	674:678	arg1	wafers					686:691	Blank (BLK) wafers	674:691	Blank (BLK) wafers (no loaded drug)	674:708	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	4	10	theme	Blank	674:678	arg1	drug					704:707	no loaded drug	694:707	no loaded drug	694:707	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	8	11	theme	ideal	1658:1662	arg1	characteristics					1664:1678	the most ideal characteristics	1649:1678	the most ideal characteristics	1649:1678	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	6	12	dep	reflectance	1214:1224	arg1	transform					1236:1244	transform	1236:1244	transform infrared (ATR-FTIR)	1236:1264	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	4	13	theme	weight	865:870	arg1	ratios					872:877	different weight ratios	855:877	different weight ratios in water	855:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	10	14	theme	DL	1882:1883	arg1	wafers					1885:1890	DL wafers	1882:1890	DL wafers	1882:1890	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	5	15	theme	%	986:986	arg1	v/v					988:990	45% v/v	984:990	45% v/v ethanol	984:998	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	3	16	theme	potential	561:569	arg1	delivery					587:594	potential oral and buccal delivery	561:594	potential oral and buccal delivery of low dose aspirin	561:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	7	17	theme	CS	1484:1485	arg1	1:1					1487:1489	CS 1:1	1484:1489	CS 1:1 (sample E2)	1484:1501	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	17	theme	CS	1484:1485	arg1	E2					1499:1500	sample E2	1492:1500	sample E2	1492:1500	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	12	18	theme	dissolution	2190:2200	arg1	studies					2202:2208	Drug dissolution studies	2185:2208	Drug dissolution studies	2185:2208	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	6	19	dep	hardness	1108:1115	arg1	mucoadhesion					1118:1129	mucoadhesion	1118:1129	mucoadhesion	1118:1129	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	3	20	theme	low	599:601	arg1	aspirin					608:614	low dose aspirin	599:614	low dose aspirin	599:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	6	21	dep	infrared	1246:1253	arg1	ATR-FTIR					1256:1263	ATR-FTIR	1256:1263	ATR-FTIR	1256:1263	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	11	22	with	values	2110:2115	arg1	saline					2139:2144	phosphate buffer saline	2122:2144	phosphate buffer saline (PBS)	2122:2150	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	11	22	with	values	2110:2115	arg1	PBS					2147:2149	PBS	2147:2149	PBS	2147:2149	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	6	23	theme	electron	1142:1149	arg1	SEM					1163:1165	SEM	1163:1165	SEM	1163:1165	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	23	theme	electron	1142:1149	arg1	microscopy					1151:1160	scanning electron microscopy	1133:1160	scanning electron microscopy (SEM)	1133:1166	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	11	24	theme	simulated	2162:2170	arg1	SS					2180:2181	SS	2180:2181	SS	2180:2181	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	11	24	theme	simulated	2162:2170	arg1	saliva					2172:2177	simulated saliva	2162:2177	simulated saliva (SS)	2162:2182	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	6	25	theme	total	1208:1212	arg1	reflectance					1214:1224	attenuated total reflectance	1197:1224	attenuated total reflectance - Fourier transform infrared (ATR-FTIR)	1197:1264	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	12	26	theme	peak	2376:2379	arg1	shifts					2381:2386	peak shifts	2376:2386	peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1	2376:2449	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	0	27	with	people	132:137	arg1	dysphagia					144:152	dysphagia	144:152	dysphagia	144:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	1	28	from	significance	213:224	arg1	decades					240:246	the coming decades	229:246	the coming decades due to low birth rates and higher life expectancy	229:296	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	13	29	with	patients	2639:2646	arg1	dysphagia					2653:2661	dysphagia	2653:2661	dysphagia	2653:2661	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	4	30	theme	polymer	909:915	arg1	combinations					917:928	optimum polymer combinations	901:928	optimum polymer combinations	901:928	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	12	31	theme	aspirin	2337:2343	arg1	interaction					2322:2332	the interaction	2318:2332	the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1	2318:2449	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	4	32	theme	BLK	681:683	arg1	wafers					686:691	Blank (BLK) wafers	674:691	Blank (BLK) wafers (no loaded drug)	674:708	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	4	32	theme	BLK	681:683	arg1	drug					704:707	no loaded drug	694:707	no loaded drug	694:707	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	4	33	from	chitosan	838:845	arg1	ratios					872:877	different weight ratios	855:877	different weight ratios in water	855:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	3	34	theme	aspirin	608:614	arg1	delivery					587:594	potential oral and buccal delivery	561:594	potential oral and buccal delivery of low dose aspirin	561:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	13	35	theme	sample	2503:2508	arg1	1:3					2498:2500	CS 1:3	2495:2500	CS 1:3 (sample DL8)	2495:2513	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	35	theme	sample	2503:2508	arg1	DL8					2510:2512	sample DL8	2503:2512	sample DL8	2503:2512	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	3	36	dep	potential	561:569	arg1	oral					571:574	oral	571:574	oral	571:574	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	7	37	theme	samples	1460:1466	arg1	B2					1468:1469	CAR 1:1 samples B2	1452:1469	CAR 1:1 samples B2	1452:1469	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	10	38	theme	polymeric	1914:1922	arg1	matrix					1924:1929	a more compact polymeric matrix	1899:1929	a more compact polymeric matrix with aspirin dispersed over the surface	1899:1969	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	0	39	theme	potential	81:89	arg1	delivery					91:98	potential delivery	81:98	potential delivery of low dose aspirin for elderly people with dysphagia	81:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	7	40	theme	CAR	1452:1454	arg1	B2					1468:1469	CAR 1:1 samples B2	1452:1469	CAR 1:1 samples B2	1452:1469	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	11	41	theme	phosphate	2122:2130	arg1	saline					2139:2144	phosphate buffer saline	2122:2144	phosphate buffer saline (PBS)	2122:2150	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	11	41	theme	phosphate	2122:2130	arg1	PBS					2147:2149	PBS	2147:2149	PBS	2147:2149	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	7	42	theme	CS	1511:1512	arg1	F2					1526:1527	sample F2	1519:1527	sample F2	1519:1527	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	42	theme	CS	1511:1512	arg1	3:1					1514:1516	CS 3:1	1511:1516	MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2)	1480:1528	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	13	43	theme	dose	2616:2619	arg1	aspirin					2621:2627	low dose aspirin	2612:2627	low dose aspirin	2612:2627	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	11	44	theme	good	1995:1998	arg1	flexibility					2000:2010	a good flexibility	1993:2010	a good flexibility required for transportation and patient handling	1993:2059	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	0	45	theme	dose	107:110	arg1	aspirin					112:118	low dose aspirin	103:118	low dose aspirin for elderly people with dysphagia	103:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	0	46	theme	composite	47:55	arg1	Development					0:10	Development	0:10	Development	0:10	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	0	46	theme	composite	47:55	arg1	characterization					27:42	functional characterization	16:42	functional characterization	16:42	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	1	47	theme	low	255:257	arg1	rates					265:269	low birth rates	255:269	low birth rates	255:269	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	0	48	theme	functional	16:25	arg1	characterization					27:42	functional characterization	16:42	functional characterization	16:42	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	13	49	theme	promising	2569:2577	arg1	system					2579:2584	a very promising system	2562:2584	a very promising system for the administration of low dose aspirin for older patients with dysphagia	2562:2661	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	7	50	theme	sample	1492:1497	arg1	1:1					1487:1489	CS 1:1	1484:1489	CS 1:1 (sample E2)	1484:1501	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	50	theme	sample	1492:1497	arg1	E2					1499:1500	sample E2	1492:1500	sample E2	1492:1500	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	2	51	theme	conditions	388:397	arg1	presence					367:374	the presence	363:374	the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging	363:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	1	52	theme	higher	275:280	arg1	expectancy					287:296	higher life expectancy	275:296	higher life expectancy	275:296	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	13	53	theme	older	2633:2637	arg1	patients					2639:2646	older patients	2633:2646	older patients with dysphagia	2633:2661	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	12	54	with	interaction	2322:2332	arg1	polymers					2354:2361	the polymers	2350:2361	the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1	2350:2449	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	10	55	theme	pores	1824:1828	arg1	presence					1812:1819	the presence	1808:1819	the presence of pores	1808:1828	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	1	56	theme	major	207:211	arg1	significance					213:224	major significance	207:224	major significance	207:224	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	7	57	theme	total	1384:1388	arg1	concentration					1398:1410	higher total polymer concentration	1377:1410	higher total polymer concentration	1377:1410	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	11	58	theme	patient	2044:2050	arg1	handling					2052:2059	patient handling	2044:2059	patient handling	2044:2059	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	2	59	theme	due	356:358	arg1	medicines					346:354	more prescribed medicines	330:354	more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging	330:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	5	60	theme	loaded	945:950	arg1	DL					953:954	DL	953:954	DL	953:954	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	5	60	theme	loaded	945:950	arg1	wafers					957:962	drug loaded (DL) wafers	940:962	drug loaded (DL) wafers	940:962	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	2	61	theme	prescribed	335:344	arg1	medicines					346:354	more prescribed medicines	330:354	more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging	330:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	6	62	theme	swelling	1346:1353	arg1	capacity					1355:1362	swelling capacity	1346:1362	swelling capacity	1346:1362	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	1	63	theme	coming	233:238	arg1	decades					240:246	the coming decades	229:246	the coming decades due to low birth rates and higher life expectancy	229:296	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	11	64	with	capacity	2088:2095	arg1	saline					2139:2144	phosphate buffer saline	2122:2144	phosphate buffer saline (PBS)	2122:2150	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	11	64	with	capacity	2088:2095	arg1	PBS					2147:2149	PBS	2147:2149	PBS	2147:2149	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	4	65	theme	low	817:819	arg1	weight					831:836	low molecular weight	817:836	low molecular weight chitosan (CS) in different weight ratios in water	817:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	11	66	theme	higher	2072:2077	arg1	capacity					2088:2095	higher swelling capacity	2072:2095	higher swelling capacity	2072:2095	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	3	67	from	thrombosis	627:636	arg1	patients					649:656	elderly patients	641:656	elderly patients with dysphagia	641:671	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	3	68	theme	composite	540:548	arg1	wafers					550:555	composite wafers	540:555	composite wafers for potential oral and buccal delivery of low dose aspirin	540:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	13	69	theme	Lyophilized	2452:2462	arg1	CAR					2464:2466	Lyophilized CAR	2452:2466	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.	2452:2662	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	1	70	theme	due	248:250	arg1	decades					240:246	the coming decades	229:246	the coming decades due to low birth rates and higher life expectancy	229:296	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	13	71	theme	CAR	2523:2525	arg1	3:1					2527:2529	CAR 3:1	2523:2529	CAR 3:1 (sample DL1)	2523:2542	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	71	theme	CAR	2523:2525	arg1	DL1					2539:2541	sample DL1	2532:2541	sample DL1	2532:2541	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	4	72	theme	weight	831:836	arg1	CS					848:849	CS	848:849	CS	848:849	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	4	72	theme	weight	831:836	arg1	chitosan					838:845	low molecular weight chitosan	817:845	low molecular weight chitosan (CS) in different weight ratios in water	817:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	10	73	theme	wafers	1867:1872	arg1	matrix					1849:1854	the polymer matrix	1837:1854	the polymer matrix of the BLK wafers	1837:1872	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	6	74	theme	X-ray	1169:1173	arg1	XRD					1191:1193	XRD	1191:1193	XRD	1191:1193	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	74	theme	X-ray	1169:1173	arg1	diffractometry					1175:1188	X-ray diffractometry	1169:1188	X-ray diffractometry (XRD)	1169:1194	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	75	theme	texture	1090:1096	arg1	analyzer					1098:1105	texture analyzer	1090:1105	texture analyzer (hardness, mucoadhesion)	1090:1130	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	75	theme	texture	1090:1096	arg1	hardness					1108:1115	hardness	1108:1115	hardness	1108:1115	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	76	theme	differential	1267:1278	arg1	DSC					1302:1304	DSC	1302:1304	DSC	1302:1304	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	76	theme	differential	1267:1278	arg1	calorimetry					1289:1299	differential scanning calorimetry	1267:1299	differential scanning calorimetry (DSC)	1267:1305	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	1	77	theme	ageing	181:186	arg1	likely					191:196	likely	191:196	likely	191:196	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	1	77	theme	ageing	181:186	arg1	impact					159:164	The impact	155:164	The impact of demographic ageing	155:186	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	8	78	dep	samples	1616:1622	arg1	F2					1639:1640	F2	1639:1640	F2	1639:1640	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	8	78	dep	samples	1616:1622	arg1	E2					1632:1633	E2	1632:1633	E2	1632:1633	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	8	78	dep	samples	1616:1622	arg1	samples					1616:1622	samples C2, B2, E2 and F2	1616:1640	samples C2, B2, E2 and F2	1616:1640	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	8	78	dep	samples	1616:1622	arg1	B2					1628:1629	B2	1628:1629	B2	1628:1629	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	8	78	dep	samples	1616:1622	arg1	C2					1624:1625	C2	1624:1625	C2	1624:1625	From the characterization, samples C2, B2, E2 and F2 showed the most ideal characteristics.
30312748	0	79	theme	elderly	124:130	arg1	people					132:137	elderly people	124:137	elderly people with dysphagia	124:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	4	80	theme	different	855:863	arg1	ratios					872:877	different weight ratios	855:877	different weight ratios in water	855:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	9	81	theme	BLK	1697:1699	arg1	wafers					1701:1706	BLK wafers	1697:1706	BLK wafers	1697:1706	XRD showed that BLK wafers were amorphous, whilst the DL wafers were crystalline due to the presence of aspirin.
30312748	5	82	theme	v/v	988:990	arg1	ethanol					992:998	45% v/v ethanol	984:998	45% v/v ethanol	984:998	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	3	83	with	patients	649:656	arg1	dysphagia					663:671	dysphagia	663:671	dysphagia	663:671	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	10	84	theme	compact	1906:1912	arg1	matrix					1924:1929	a more compact polymeric matrix	1899:1929	a more compact polymeric matrix with aspirin dispersed over the surface	1899:1969	SEM confirmed the presence of pores within the polymer matrix of the BLK wafers, whilst DL wafers showed a more compact polymeric matrix with aspirin dispersed over the surface.
30312748	3	85	theme	buccal	580:585	arg1	delivery					587:594	potential oral and buccal delivery	561:594	potential oral and buccal delivery of low dose aspirin	561:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	6	86	dep	transform	1236:1244	arg1	infrared					1246:1253	infrared	1246:1253	transform infrared (ATR-FTIR)	1236:1264	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	87	theme	scanning	1133:1140	arg1	SEM					1163:1165	SEM	1163:1165	SEM	1163:1165	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	87	theme	scanning	1133:1140	arg1	microscopy					1151:1160	scanning electron microscopy	1133:1160	scanning electron microscopy (SEM)	1133:1166	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	88	theme	attenuated	1197:1206	arg1	reflectance					1214:1224	attenuated total reflectance	1197:1224	attenuated total reflectance - Fourier transform infrared (ATR-FTIR)	1197:1264	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	7	89	dep	MET	1448:1450	arg1	B2					1468:1469	CAR 1:1 samples B2	1452:1469	CAR 1:1 samples B2	1452:1469	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	13	90	dep	1:3	2472:2474	arg1	1:3					2498:2500	CS 1:3	2495:2500	CS 1:3 (sample DL8)	2495:2513	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	90	dep	1:3	2472:2474	arg1	DL8					2510:2512	sample DL8	2503:2512	sample DL8	2503:2512	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	90	dep	1:3	2472:2474	arg1	MET					2519:2521	MET	2519:2521	MET	2519:2521	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	90	dep	1:3	2472:2474	arg1	3:1					2527:2529	CAR 3:1	2523:2529	CAR 3:1 (sample DL1)	2523:2542	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	90	dep	1:3	2472:2474	arg1	DL1					2539:2541	sample DL1	2532:2541	sample DL1	2532:2541	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	91	theme	aspirin	2621:2627	arg1	administration					2594:2607	the administration	2590:2607	the administration of low dose aspirin	2590:2627	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	4	92	theme	loaded	697:702	arg1	wafers					686:691	Blank (BLK) wafers	674:691	Blank (BLK) wafers (no loaded drug)	674:708	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	4	92	theme	loaded	697:702	arg1	drug					704:707	no loaded drug	694:707	no loaded drug	694:707	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	5	93	theme	45	984:985	arg1	%					986:986	%	986:986	%	986:986	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	3	94	theme	dose	603:606	arg1	aspirin					608:614	low dose aspirin	599:614	low dose aspirin	599:614	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	7	95	theme	1:1	1456:1458	arg1	B2					1468:1469	CAR 1:1 samples B2	1452:1469	CAR 1:1 samples B2	1452:1469	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	4	96	theme	optimum	901:907	arg1	combinations					917:928	optimum polymer combinations	901:928	optimum polymer combinations	901:928	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	6	97	theme	scanning	1280:1287	arg1	DSC					1302:1304	DSC	1302:1304	DSC	1302:1304	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	97	theme	scanning	1280:1287	arg1	calorimetry					1289:1299	differential scanning calorimetry	1267:1299	differential scanning calorimetry (DSC)	1267:1305	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	5	98	theme	aspirin	1039:1045	arg1	solubilization					1017:1030	complete solubilization	1008:1030	complete solubilization of the aspirin	1008:1045	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	12	99	theme	Drug	2185:2188	arg1	studies					2202:2208	Drug dissolution studies	2185:2208	Drug dissolution studies	2185:2208	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	12	100	theme	broad	2413:2417	arg1	peak					2419:2422	the broad peak	2409:2422	the broad peak between 2500 and 3300 cm-1	2409:2449	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	0	101	theme	low	103:105	arg1	aspirin					112:118	low dose aspirin	103:118	low dose aspirin for elderly people with dysphagia	103:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	13	102	dep	CAR	2464:2466	arg1	wafers					2544:2549	wafers	2544:2549	wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia	2544:2661	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	7	103	theme	sample	1519:1524	arg1	F2					1526:1527	sample F2	1519:1527	sample F2	1519:1527	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	103	theme	sample	1519:1524	arg1	3:1					1514:1516	CS 3:1	1511:1516	MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2)	1480:1528	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	13	104	theme	low	2612:2614	arg1	aspirin					2621:2627	low dose aspirin	2612:2627	low dose aspirin	2612:2627	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	11	105	theme	adhesion	2101:2108	arg1	values					2110:2115	adhesion values	2101:2115	adhesion values with phosphate buffer saline (PBS)	2101:2150	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	0	106	theme	aspirin	112:118	arg1	delivery					91:98	potential delivery	81:98	potential delivery of low dose aspirin for elderly people with dysphagia	81:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	5	107	theme	complete	1008:1015	arg1	solubilization					1017:1030	complete solubilization	1008:1030	complete solubilization of the aspirin	1008:1045	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	13	108	theme	CS	2495:2496	arg1	1:3					2498:2500	CS 1:3	2495:2500	CS 1:3 (sample DL8)	2495:2513	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	108	theme	CS	2495:2496	arg1	DL8					2510:2512	sample DL8	2503:2512	sample DL8	2503:2512	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	9	109	theme	aspirin	1785:1791	arg1	presence					1773:1780	the presence	1769:1780	the presence of aspirin	1769:1791	XRD showed that BLK wafers were amorphous, whilst the DL wafers were crystalline due to the presence of aspirin.
30312748	7	110	dep	MET	1480:1482	arg1	F2					1526:1527	sample F2	1519:1527	sample F2	1519:1527	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	110	dep	MET	1480:1482	arg1	1:1					1487:1489	CS 1:1	1484:1489	CS 1:1 (sample E2)	1484:1501	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	110	dep	MET	1480:1482	arg1	E2					1499:1500	sample E2	1492:1500	sample E2	1492:1500	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	110	dep	MET	1480:1482	arg1	MET					1507:1509	MET	1507:1509	MET	1507:1509	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	110	dep	MET	1480:1482	arg1	3:1					1514:1516	CS 3:1	1511:1516	MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2)	1480:1528	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	13	111	theme	sample	2477:2482	arg1	DL13					2484:2487	sample DL13	2477:2487	sample DL13	2477:2487	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	111	theme	sample	2477:2482	arg1	1:3					2472:2474	CS 1:3	2469:2474	CS 1:3 (sample DL13)	2469:2488	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	3	112	dep	development	490:500	arg1	the					486:488	the	486:488	the	486:488	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	11	113	theme	buffer	2132:2137	arg1	saline					2139:2144	phosphate buffer saline	2122:2144	phosphate buffer saline (PBS)	2122:2150	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	11	113	theme	buffer	2132:2137	arg1	PBS					2147:2149	PBS	2147:2149	PBS	2147:2149	The DL wafers showed a good flexibility required for transportation and patient handling and showed higher swelling capacity and adhesion values with phosphate buffer saline (PBS) than with simulated saliva (SS).
30312748	4	114	from	ratios	872:877	arg1	water					882:886	water	882:886	water	882:886	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	1	115	theme	birth	259:263	arg1	rates					265:269	low birth rates	255:269	low birth rates	255:269	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	3	116	theme	elderly	641:647	arg1	patients					649:656	elderly patients	641:656	elderly patients with dysphagia	641:671	This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia.
30312748	6	117	theme	thermogravimetric	1308:1324	arg1	TGA					1336:1338	TGA	1336:1338	TGA	1336:1338	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	6	117	theme	thermogravimetric	1308:1324	arg1	analyzer					1326:1333	thermogravimetric analyzer	1308:1333	thermogravimetric analyzer (TGA)	1308:1339	The formulations were characterized using texture analyzer (hardness, mucoadhesion), scanning electron microscopy (SEM), X-ray diffractometry (XRD), attenuated total reflectance - Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetric analyzer (TGA), and swelling capacity.
30312748	2	118	theme	challenging	453:463	arg1	medicines					443:451	swallowing medicines	432:451	swallowing medicines challenging	432:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	2	119	theme	multiple	379:386	arg1	conditions					388:397	multiple conditions	379:397	multiple conditions such as dysphagia which can make swallowing medicines challenging	379:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	2	119	theme	multiple	379:386	arg1	dysphagia					407:415	dysphagia	407:415	dysphagia which can make swallowing medicines challenging	407:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	0	120	theme	dried	64:68	arg1	wafers					70:75	dried wafers	64:75	dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia	64:152	Development and functional characterization of composite freeze dried wafers for potential delivery of low dose aspirin for elderly people with dysphagia.
30312748	1	121	theme	life	282:285	arg1	expectancy					287:296	higher life expectancy	275:296	higher life expectancy	275:296	The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy.
30312748	2	122	theme	swallowing	432:441	arg1	medicines					443:451	swallowing medicines	432:451	swallowing medicines challenging	432:463	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	12	123	theme	first	2258:2262	arg1	20 min					2264:2269	the first 20 min	2254:2269	the first 20 min	2254:2269	Drug dissolution studies showed that aspirin was rapidly released in the first 20 min and then continuously over 1 h. FTIR confirmed the interaction of aspirin with the polymers evidenced by peak shifts around 1750 cm-1 and the broad peak between 2500 and 3300 cm-1.
30312748	4	124	theme	metolose	766:773	arg1	combinations					750:761	combinations	750:761	combinations of metolose (MET)	750:779	Blank (BLK) wafers (no loaded drug) were initially formulated by dissolving combinations of metolose (MET) with carrageenan (CAR) and MET with low molecular weight chitosan (CS) in different weight ratios in water, to identify optimum polymer combinations.
30312748	2	125	theme	Older	299:303	arg1	people					305:310	Older people	299:310	Older people generally	299:320	Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging.
30312748	13	126	theme	CS	2469:2470	arg1	DL13					2484:2487	sample DL13	2477:2487	sample DL13	2477:2487	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	126	theme	CS	2469:2470	arg1	MET					2491:2493	MET	2491:2493	MET	2491:2493	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	13	126	theme	CS	2469:2470	arg1	1:3					2472:2474	CS 1:3	2469:2474	CS 1:3 (sample DL13)	2469:2488	Lyophilized CAR: CS 1:3 (sample DL13), MET:CS 1:3 (sample DL8) and MET:CAR 3:1 (sample DL1) wafers seem to be a very promising system for the administration of low dose aspirin for older patients with dysphagia.
30312748	7	127	theme	polymer	1390:1396	arg1	concentration					1398:1410	higher total polymer concentration	1377:1410	higher total polymer concentration	1377:1410	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	5	128	theme	drug	940:943	arg1	DL					953:954	DL	953:954	DL	953:954	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	5	128	theme	drug	940:943	arg1	wafers					957:962	drug loaded (DL) wafers	940:962	drug loaded (DL) wafers	940:962	However, drug loaded (DL) wafers were prepared using 45% v/v ethanol to help complete solubilization of the aspirin.
30312748	7	129	dep	penetration	1435:1445	arg1	C2					1472:1473	C2	1472:1473	C2	1472:1473	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
30312748	7	129	dep	penetration	1435:1445	arg1	MET					1448:1450	MET	1448:1450	MET	1448:1450	Wafers with higher total polymer concentration were more resistant to penetration (MET:CAR 1:1 samples B2, C2) and MET:CS 1:1 (sample E2) and MET:CS 3:1 (sample F2) and also depended on the ratios between the polymers used.
31336278	8	0	theme	bacterial	1563:1571	arg1	resistance					1573:1582	bacterial resistance	1563:1582	bacterial resistance	1563:1582	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	4	1	theme	antimicrobial	702:714	arg1	peptides					716:723	antimicrobial peptides	702:723	antimicrobial peptides	702:723	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	6	2	theme	enhanced	1061:1068	arg1	property					1084:1091	The enhanced antimicrobial property	1057:1091	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs	1057:1142	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	2	3	theme	antimicrobial	482:494	arg1	properties					496:505	the cellular and antimicrobial properties	465:505	the cellular and antimicrobial properties of the probiotics	465:523	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	2	4	from	effects	454:460	arg1	properties					496:505	the cellular and antimicrobial properties	465:505	the cellular and antimicrobial properties of the probiotics	465:523	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	6	5	theme	pathogens	1173:1181	arg1	number					1163:1168	the number	1159:1168	the number of pathogens	1159:1181	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	2	6	theme	probiotics	306:315	arg1	activity					294:301	the activity	290:301	the activity of probiotics	290:315	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	3	7	theme	PDNs	570:573	arg1	dependent					593:601	dependent	593:601	dependent	593:601	First, we found that the internalization of PDNs by probiotics was dependent on temperature, time, and glucose transporters.
31336278	3	7	theme	PDNs	570:573	arg1	internalization					551:565	the internalization	547:565	the internalization of PDNs by probiotics	547:587	First, we found that the internalization of PDNs by probiotics was dependent on temperature, time, and glucose transporters.
31336278	5	8	theme	gut	1033:1035	arg1	microbiome					1037:1046	gut microbiome	1033:1046	gut microbiome	1033:1046	Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo.
31336278	6	9	theme	beneficial	1196:1205	arg1	species					1216:1222	beneficial bacteria species	1196:1222	beneficial bacteria species in mice	1196:1230	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	7	10	theme	microbiome	1269:1278	arg1	composition					1250:1260	the composition	1246:1260	the composition of gut microbiome	1246:1278	Furthermore, the composition of gut microbiome was changed, and resulted in preventing reduction of the diversity of the microflora.
31336278	7	11	theme	microflora	1354:1363	arg1	diversity					1337:1345	the diversity	1333:1345	the diversity of the microflora	1333:1363	Furthermore, the composition of gut microbiome was changed, and resulted in preventing reduction of the diversity of the microflora.
31336278	5	12	theme	microbiome	1037:1046	arg1	population					1019:1028	the population	1015:1028	the population of gut microbiome	1015:1046	Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo.
31336278	1	13	theme	overuse	189:195	arg1	use					197:199	overuse use	189:199	overuse use of antibiotics	189:214	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	1	14	theme	antibiotics	169:179	arg1	attention					138:146	much attention	133:146	much attention	133:146	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	1	14	theme	antibiotics	169:179	arg1	alternative					154:164	an alternative	151:164	an alternative of antibiotics	151:179	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	2	15	theme	probiotics	514:523	arg1	properties					496:505	the cellular and antimicrobial properties	465:505	the cellular and antimicrobial properties of the probiotics	465:523	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	6	16	theme	bacteria	1207:1214	arg1	species					1216:1222	beneficial bacteria species	1196:1222	beneficial bacteria species in mice	1196:1230	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	4	17	theme	antimicrobial	793:805	arg1	activities					807:816	higher antimicrobial activities	786:816	higher antimicrobial activities against Gram-positive and -negative pathogens	786:862	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	2	18	theme	phthalyl	330:337	arg1	PDNs					362:365	PDNs	362:365	PDNs	362:365	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	2	18	theme	phthalyl	330:337	arg1	nanoparticles					347:359	phthalyl dextran nanoparticles	330:359	phthalyl dextran nanoparticles (PDNs)	330:366	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	2	19	theme	anhydride	395:403	arg1	conjugation					371:381	conjugation	371:381	conjugation of phthalic anhydride with dextran	371:416	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	0	20	theme	Novel	0:4	arg1	production					6:15	Novel production	0:15	Novel production of natural bacteriocin via internalization of dextran	0:69	Novel production of natural bacteriocin via internalization of dextran nanoparticles into probiotics.
31336278	4	21	theme	self-defense	747:758	arg1	mechanism					760:768	self-defense mechanism	747:768	self-defense mechanism	747:768	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	2	22	theme	phthalic	386:393	arg1	anhydride					395:403	phthalic anhydride	386:403	phthalic anhydride	386:403	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	0	23	theme	natural	20:26	arg1	bacteriocin					28:38	natural bacteriocin	20:38	natural bacteriocin	20:38	Novel production of natural bacteriocin via internalization of dextran nanoparticles into probiotics.
31336278	8	24	theme	probiotic	1437:1445	arg1	metabolism					1457:1466	probiotic bacterial metabolism	1437:1466	probiotic bacterial metabolism	1437:1466	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	8	25	theme	bacterial	1447:1455	arg1	metabolism					1457:1466	probiotic bacterial metabolism	1437:1466	probiotic bacterial metabolism	1437:1466	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	1	26	theme	antibiotics	204:214	arg1	use					197:199	overuse use	189:199	overuse use of antibiotics	189:214	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	8	27	theme	prebiotic	1414:1422	arg1	type					1406:1409	a new type	1400:1409	a new type of prebiotic	1400:1422	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	5	28	theme	pathogenic	979:988	arg1	infections					994:1003	pathogenic gut infections	979:1003	pathogenic gut infections	979:1003	Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo.
31336278	7	29	theme	gut	1265:1267	arg1	microbiome					1269:1278	gut microbiome	1265:1278	gut microbiome	1265:1278	Furthermore, the composition of gut microbiome was changed, and resulted in preventing reduction of the diversity of the microflora.
31336278	4	30	theme	PDNs	670:673	arg1	Internalization					651:665	Internalization	651:665	Internalization of PDNs	651:673	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	0	31	theme	bacteriocin	28:38	arg1	production					6:15	Novel production	0:15	Novel production of natural bacteriocin via internalization of dextran	0:69	Novel production of natural bacteriocin via internalization of dextran nanoparticles into probiotics.
31336278	2	32	theme	cellular	469:476	arg1	properties					496:505	the cellular and antimicrobial properties	465:505	the cellular and antimicrobial properties of the probiotics	465:523	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	6	33	theme	acidilactidi	1108:1119	arg1	property					1084:1091	The enhanced antimicrobial property	1057:1091	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs	1057:1142	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	3	34	theme	glucose	629:635	arg1	transporters					637:648	glucose transporters	629:648	glucose transporters	629:648	First, we found that the internalization of PDNs by probiotics was dependent on temperature, time, and glucose transporters.
31336278	8	35	theme	new	1482:1484	arg1	avenue					1486:1491	a new avenue	1480:1491	a new avenue for probiotic modulation	1480:1516	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	4	36	dep	resulted	774:781	arg1	compared					864:871	compared	864:871	compared to probiotics themselves	864:896	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	4	37	theme	peptides	716:723	arg1	production					688:697	the production	684:697	the production of antimicrobial peptides by probiotics	684:737	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	7	38	theme	diversity	1337:1345	arg1	reduction					1320:1328	reduction	1320:1328	reduction of the diversity of the microflora	1320:1363	Furthermore, the composition of gut microbiome was changed, and resulted in preventing reduction of the diversity of the microflora.
31336278	4	39	theme	Gram-positive	826:838	arg1	pathogens					854:862	Gram-positive and -negative pathogens	826:862	Gram-positive and -negative pathogens	826:862	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	6	40	from	species	1216:1222	arg1	mice					1227:1230	mice	1227:1230	mice	1227:1230	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	1	41	theme	much	133:136	arg1	attention					138:146	much attention	133:146	much attention	133:146	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	1	41	theme	much	133:136	arg1	alternative					154:164	an alternative	151:164	an alternative of antibiotics	151:179	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	5	42	theme	PDN-internalized	930:945	arg1	probiotics					947:956	PDN-internalized probiotics	930:956	PDN-internalized probiotics	930:956	Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo.
31336278	1	43	theme	widespread	227:236	arg1	resistance					248:257	widespread bacterial resistance	227:257	widespread bacterial resistance	227:257	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	5	44	theme	gut	990:992	arg1	infections					994:1003	pathogenic gut infections	979:1003	pathogenic gut infections	979:1003	Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo.
31336278	4	45	theme	higher	786:791	arg1	activities					807:816	higher antimicrobial activities	786:816	higher antimicrobial activities against Gram-positive and -negative pathogens	786:862	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	2	46	theme	dextran	339:345	arg1	PDNs					362:365	PDNs	362:365	PDNs	362:365	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	2	46	theme	dextran	339:345	arg1	nanoparticles					347:359	phthalyl dextran nanoparticles	330:359	phthalyl dextran nanoparticles (PDNs)	330:366	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	8	47	theme	new	1402:1404	arg1	type					1406:1409	a new type	1400:1409	a new type of prebiotic	1400:1422	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	1	48	theme	bacterial	238:246	arg1	resistance					248:257	widespread bacterial resistance	227:257	widespread bacterial resistance	227:257	Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance.
31336278	2	49	with	conjugation	371:381	arg1	dextran					410:416	dextran	410:416	dextran	410:416	Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics.
31336278	4	50	theme	-negative	844:852	arg1	pathogens					854:862	Gram-positive and -negative pathogens	826:862	Gram-positive and -negative pathogens	826:862	Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves.
31336278	0	51	theme	dextran	63:69	arg1	internalization					44:58	internalization	44:58	internalization of dextran	44:69	Novel production of natural bacteriocin via internalization of dextran nanoparticles into probiotics.
31336278	8	52	theme	resistance	1573:1582	arg1	challenge					1550:1558	the challenge	1546:1558	the challenge of bacterial resistance	1546:1582	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	8	53	theme	probiotic	1497:1505	arg1	modulation					1507:1516	probiotic modulation	1497:1516	probiotic modulation	1497:1516	Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.
31336278	6	54	theme	Pediococcus	1096:1106	arg1	acidilactidi					1108:1119	Pediococcus acidilactidi	1096:1119	Pediococcus acidilactidi internalized with PDNs	1096:1142	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
31336278	6	55	theme	antimicrobial	1070:1082	arg1	property					1084:1091	The enhanced antimicrobial property	1057:1091	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs	1057:1142	The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice.
30177171	2	0	theme	KC	466:467	arg1	presence					454:461	the presence	450:461	the presence of KC in water	450:476	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	3	1	from	water	616:620	arg1	separation					526:535	separation	526:535	separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water	526:620	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	4	2	theme	process	641:647	arg1	parameters					649:658	The synthesis and process parameters	623:658	The synthesis and process parameters for adsorption	623:673	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	3	3	dep	T	550:550	arg1	ST					553:554	ST	553:554	ST	553:554	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	3	3	dep	T	550:550	arg1	mixtures					602:609	mixtures	602:609	mixtures	602:609	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	3	4	theme	cationic	589:596	arg1	dye					598:600	brilliant cresyl blue (BCB) cationic dye	561:600	brilliant cresyl blue (BCB) cationic dye	561:600	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	5	5	theme	MBA	871:873	arg1	1.3:1					902:906	1.3:1	902:906	1.3:1	902:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	5	theme	MBA	871:873	arg1	KC					856:857	KC	856:857	KC	856:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	5	theme	MBA	871:873	arg1	NVP					879:881	NVP	879:881	NVP	879:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	5	theme	MBA	871:873	arg1	%					866:866	1.5 wt%	860:866	1.5 wt% of MBA and NVP	860:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	5	theme	MBA	871:873	arg1	MBA					871:873	MBA	871:873	MBA	871:873	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	5	theme	MBA	871:873	arg1	%					851:851	2.5 wt%	845:851	2.5 wt% of KC	845:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	6	theme	BCB	979:981	arg1	dye					983:985	BCB dye	979:985	BCB dye	979:985	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	4	7	theme	synthesis	627:635	arg1	parameters					649:658	The synthesis and process parameters	623:658	The synthesis and process parameters for adsorption	623:673	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	5	8	theme	NVP	879:881	arg1	1.3:1					902:906	1.3:1	902:906	1.3:1	902:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	8	theme	NVP	879:881	arg1	KC					856:857	KC	856:857	KC	856:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	8	theme	NVP	879:881	arg1	NVP					879:881	NVP	879:881	NVP	879:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	8	theme	NVP	879:881	arg1	%					866:866	1.5 wt%	860:866	1.5 wt% of MBA and NVP	860:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	8	theme	NVP	879:881	arg1	MBA					871:873	MBA	871:873	MBA	871:873	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	8	theme	NVP	879:881	arg1	%					851:851	2.5 wt%	845:851	2.5 wt% of KC	845:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	9	theme	adsorbent	1070:1078	arg1	dose					1080:1083	adsorbent dose	1070:1083	adsorbent dose of 0.25 g/L	1070:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	0	10	theme	surface	110:116	arg1	methodology					118:128	response surface methodology	101:128	response surface methodology	101:128	Efficient removal of cationic dye mixtures from water using a bio-composite adsorbent optimized with response surface methodology.
30177171	5	11	theme	dye	983:985	arg1	398 mg/g					967:974	398 mg/g	967:974	398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	967:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	2	12	from	monomers	337:344	arg1	free					313:316	free	313:316	free	313:316	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	5	13	dep	%	851:851	arg1	ratio					893:897	AA molar ratio	884:897	AA molar ratio	884:897	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	1.3:1					902:906	1.3:1	902:906	1.3:1	902:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	KC					856:857	KC	856:857	KC	856:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	NVP					879:881	NVP	879:881	NVP	879:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	%					866:866	1.5 wt%	860:866	1.5 wt% of MBA and NVP	860:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	MBA					871:873	MBA	871:873	MBA	871:873	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	14	theme	KC	856:857	arg1	%					851:851	2.5 wt%	845:851	2.5 wt% of KC	845:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	15	theme	CPKC	786:789	arg1	bio-composite					791:803	the CPKC bio-composite adsorbent	782:813	the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1	782:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	0	16	theme	response	101:108	arg1	methodology					118:128	response surface methodology	101:128	response surface methodology	101:128	Efficient removal of cationic dye mixtures from water using a bio-composite adsorbent optimized with response surface methodology.
30177171	2	17	from	presence	454:461	arg1	water					472:476	water	472:476	water	472:476	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	5	18	theme	AA	884:885	arg1	ratio					893:897	AA molar ratio	884:897	AA molar ratio	884:897	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	2	19	theme	crosslinker	425:435	arg1	comonomer					437:445	crosslinker comonomer	425:445	crosslinker comonomer	425:445	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	5	20	theme	2.5 wt	845:850	arg1	1.3:1					902:906	1.3:1	902:906	1.3:1	902:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	20	theme	2.5 wt	845:850	arg1	KC					856:857	KC	856:857	KC	856:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	20	theme	2.5 wt	845:850	arg1	NVP					879:881	NVP	879:881	NVP	879:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	20	theme	2.5 wt	845:850	arg1	%					866:866	1.5 wt%	860:866	1.5 wt% of MBA and NVP	860:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	20	theme	2.5 wt	845:850	arg1	MBA					871:873	MBA	871:873	MBA	871:873	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	20	theme	2.5 wt	845:850	arg1	%					851:851	2.5 wt%	845:851	2.5 wt% of KC	845:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	3	21	theme	Safranine	540:548	arg1	T					550:550	Safranine T	540:550	Safranine T (ST)	540:555	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	5	22	theme	adsorbent	805:813	arg1	bio-composite					791:803	the CPKC bio-composite adsorbent	782:813	the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1	782:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	0	23	theme	Efficient	0:8	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of cationic dye	0:32	Efficient removal of cationic dye mixtures from water using a bio-composite adsorbent optimized with response surface methodology.
30177171	2	24	theme	free	313:316	arg1	NVP					366:368	NVP	366:368	NVP	366:368	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	2	24	theme	free	313:316	arg1	N-vinylpyrrolidone					346:363	free radically from its monomers N-vinylpyrrolidone	313:363	free radically from its monomers N-vinylpyrrolidone (NVP)	313:369	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	3	25	used	used	517:520	arg2	adsorbents					483:492	The adsorbents	479:492	The adsorbents	479:492	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	0	26	theme	cationic	21:28	arg1	dye					30:32	cationic dye	21:32	cationic dye	21:32	Efficient removal of cationic dye mixtures from water using a bio-composite adsorbent optimized with response surface methodology.
30177171	4	27	theme	surface	742:748	arg1	RSM					763:765	RSM	763:765	RSM	763:765	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	4	27	theme	surface	742:748	arg1	methodology					750:760	the response surface methodology	729:760	the response surface methodology (RSM)	729:766	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	2	28	dep	N-vinylpyrrolidone	346:363	arg1	comonomer					437:445	crosslinker comonomer	425:445	crosslinker comonomer	425:445	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	3	29	theme	T	550:550	arg1	separation					526:535	separation	526:535	separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water	526:620	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	5	30	theme	solution	1049:1056	arg1	pH					1058:1059	a solution pH	1047:1059	a solution pH of 7	1047:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	2	31	from	KC	466:467	arg1	water					472:476	water	472:476	water	472:476	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	4	32	theme	central	695:701	arg1	CCD					721:723	CCD	721:723	CCD	721:723	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	4	32	theme	central	695:701	arg1	design					713:718	central composite design	695:718	central composite design (CCD) of the response surface methodology (RSM)	695:766	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	5	33	from	100 mg/L	1019:1026	arg1	pH					1058:1059	a solution pH	1047:1059	a solution pH of 7	1047:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	2	34	theme	methylene	394:402	arg1	MBA					420:422	MBA	420:422	MBA	420:422	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	2	34	theme	methylene	394:402	arg1	bis-acrylamide					404:417	methylene bis-acrylamide	394:417	methylene bis-acrylamide (MBA)	394:423	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	5	35	theme	1.3:1	902:906	arg1	1.3:1					902:906	1.3:1	902:906	1.3:1	902:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	35	theme	1.3:1	902:906	arg1	KC					856:857	KC	856:857	KC	856:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	35	theme	1.3:1	902:906	arg1	NVP					879:881	NVP	879:881	NVP	879:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	35	theme	1.3:1	902:906	arg1	%					866:866	1.5 wt%	860:866	1.5 wt% of MBA and NVP	860:881	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	35	theme	1.3:1	902:906	arg1	MBA					871:873	MBA	871:873	MBA	871:873	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	35	theme	1.3:1	902:906	arg1	%					851:851	2.5 wt%	845:851	2.5 wt% of KC	845:857	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	3	36	theme	dye	598:600	arg1	separation					526:535	separation	526:535	separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water	526:620	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	2	37	theme	acrylic	372:378	arg1	AA					386:387	AA	386:387	AA	386:387	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	2	37	theme	acrylic	372:378	arg1	acid					380:383	acrylic acid	372:383	acrylic acid (AA)	372:388	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	0	38	theme	dye	30:32	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of cationic dye	0:32	Efficient removal of cationic dye mixtures from water using a bio-composite adsorbent optimized with response surface methodology.
30177171	5	39	theme	7	1064:1064	arg1	pH					1058:1059	a solution pH	1047:1059	a solution pH of 7	1047:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	2	40	from	water	472:476	arg1	presence					454:461	the presence	450:461	the presence of KC in water	450:476	The CP was synthesized free radically from its monomers N-vinylpyrrolidone (NVP), acrylic acid (AA) and methylene bis-acrylamide (MBA) crosslinker comonomer in the presence of KC in water.
30177171	4	41	theme	methodology	750:760	arg1	CCD					721:723	CCD	721:723	CCD	721:723	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	4	41	theme	methodology	750:760	arg1	design					713:718	central composite design	695:718	central composite design (CCD) of the response surface methodology (RSM)	695:766	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	5	42	theme	dye	1040:1042	arg1	dose					1080:1083	adsorbent dose	1070:1083	adsorbent dose of 0.25 g/L	1070:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	42	theme	dye	1040:1042	arg1	100 mg/L					1019:1026	100 mg/L	1019:1026	100 mg/L each of the dye in a solution pH of 7	1019:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	43	theme	optimized	820:828	arg1	composition					830:840	optimized composition	820:840	optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1	820:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	44	dep	100 mg/L	1019:1026	arg1	each					1028:1031	each	1028:1031	each	1028:1031	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	45	theme	high	917:920	arg1	capacity					933:940	a high adsorption capacity	915:940	a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	915:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	46	theme	%	851:851	arg1	composition					830:840	optimized composition	820:840	optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1	820:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	47	theme	adsorption	922:931	arg1	capacity					933:940	a high adsorption capacity	915:940	a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	915:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	3	48	theme	BCB	584:586	arg1	dye					598:600	brilliant cresyl blue (BCB) cationic dye	561:600	brilliant cresyl blue (BCB) cationic dye	561:600	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	4	49	theme	response	733:740	arg1	RSM					763:765	RSM	763:765	RSM	763:765	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	4	49	theme	response	733:740	arg1	methodology					750:760	the response surface methodology	729:760	the response surface methodology (RSM)	729:766	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	5	50	from	dose	1080:1083	arg1	pH					1058:1059	a solution pH	1047:1059	a solution pH of 7	1047:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	51	theme	molar	887:891	arg1	ratio					893:897	AA molar ratio	884:897	AA molar ratio	884:897	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	3	52	theme	blue	578:581	arg1	dye					598:600	brilliant cresyl blue (BCB) cationic dye	561:600	brilliant cresyl blue (BCB) cationic dye	561:600	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	1	53	theme	Bio-composite	131:143	arg1	adsorbents					161:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	4	54	theme	composite	703:711	arg1	CCD					721:723	CCD	721:723	CCD	721:723	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	4	54	theme	composite	703:711	arg1	design					713:718	central composite design	695:718	central composite design (CCD) of the response surface methodology (RSM)	695:766	The synthesis and process parameters for adsorption were optimized with central composite design (CCD) of the response surface methodology (RSM).
30177171	5	55	with	bio-composite	791:803	arg1	composition					830:840	optimized composition	820:840	optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1	820:906	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	56	theme	362.5 mg/g	945:954	arg1	capacity					933:940	a high adsorption capacity	915:940	a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	915:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	1	57	theme	type	145:148	arg1	adsorbents					161:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	1	58	theme	poly	242:245	arg1	kappa-carrageenan					201:217	kappa-carrageenan	201:217	kappa-carrageenan (KC)	201:222	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	1	58	theme	poly	242:245	arg1	KC					220:221	KC	220:221	KC	220:221	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	1	58	theme	poly	242:245	arg1	copolymers					228:237	copolymers	228:237	copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP)	228:287	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	3	59	theme	brilliant	561:569	arg1	dye					598:600	brilliant cresyl blue (BCB) cationic dye	561:600	brilliant cresyl blue (BCB) cationic dye	561:600	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	1	60	theme	functional	150:159	arg1	adsorbents					161:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents	131:170	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	5	61	theme	0.25 g/L	1088:1095	arg1	dose					1080:1083	adsorbent dose	1070:1083	adsorbent dose of 0.25 g/L	1070:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	61	theme	0.25 g/L	1088:1095	arg1	100 mg/L					1019:1026	100 mg/L	1019:1026	100 mg/L each of the dye in a solution pH of 7	1019:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	62	contain	containing	1008:1017	arg2	100 mg/L					1019:1026	100 mg/L	1019:1026	100 mg/L each of the dye in a solution pH of 7	1019:1064	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	62	contain	containing	1008:1017	arg1	mixture					1000:1006	a binary mixture	991:1006	a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	991:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	62	contain	containing	1008:1017	arg2	dose					1080:1083	adsorbent dose	1070:1083	adsorbent dose of 0.25 g/L	1070:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	5	63	theme	binary	993:998	arg1	mixture					1000:1006	a binary mixture	991:1006	a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L	991:1095	Accordingly, the CPKC bio-composite adsorbent with optimized composition of 2.5 wt% of KC, 1.5 wt% of MBA and NVP: AA molar ratio of 1.3:1 showed a high adsorption capacity of 362.5 mg/g for ST and 398 mg/g of BCB dye for a binary mixture containing 100 mg/L each of the dye in a solution pH of 7 and adsorbent dose of 0.25 g/L.
30177171	3	64	theme	cresyl	571:576	arg1	dye					598:600	brilliant cresyl blue (BCB) cationic dye	561:600	brilliant cresyl blue (BCB) cationic dye	561:600	The adsorbents were characterized and used for separation of Safranine T (ST) and brilliant cresyl blue (BCB) cationic dye mixtures from water.
30177171	1	65	theme	N-vinylpyrrolidone-co-acrylic	248:276	arg1	poly					242:245	poly	242:245	poly (N-vinylpyrrolidone-co-acrylic acid) (CP)	242:287	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30177171	1	65	theme	N-vinylpyrrolidone-co-acrylic	248:276	arg1	acid					278:281	N-vinylpyrrolidone-co-acrylic acid	248:281	N-vinylpyrrolidone-co-acrylic acid	248:281	Bio-composite type functional adsorbents were prepared by integrating kappa-carrageenan (KC) and copolymers of poly (N-vinylpyrrolidone-co-acrylic acid) (CP).
30009765	4	0	dep	%	645:645	arg1	CS					668:669	CS	668:669	CS	668:669	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	0	dep	%	645:645	arg1	sulfate					659:665	chondroitin sulfate	647:665	67.2% chondroitin sulfate (CS)	641:670	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	0	1	from	structure	16:24	arg1	DBS					51:53	DBS	51:53	DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	51:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	0	1	from	structure	16:24	arg1	newborns					72:79	2-3-day-old newborns	60:79	2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	60:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	4	2	theme	2.35	747:750	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	6	3	theme	MPS	1145:1147	arg1	defect					1159:1164	the MPS enzymatic defect	1141:1164	the MPS enzymatic defect	1141:1164	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	4	4	dep	%	677:677	arg1	HS					696:697	HS	696:697	HS	696:697	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	4	dep	%	677:677	arg1	sulfate					687:693	heparan sulfate	679:693	28.6% heparan sulfate (HS)	673:698	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	5	5	dep	CS	818:819	arg1	structure					828:836	structure	828:836	structure	828:836	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	4	6	with	%	645:645	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	6	7	theme	different	955:963	arg1	types					969:973	four different MPS types	950:973	four different MPS types	950:973	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	0	8	from	Composition	0:10	arg1	DBS					51:53	DBS	51:53	DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	51:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	0	8	from	Composition	0:10	arg1	newborns					72:79	2-3-day-old newborns	60:79	2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	60:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	6	9	theme	UA-GAGs	1016:1022	arg1	content					1024:1030	total or single UA-GAGs content	1000:1030	content	1024:1030	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	5	10	theme	CS	818:819	arg1	evaluation					804:813	A chemical evaluation	793:813	A chemical evaluation of CS and HS structure	793:836	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	6	11	theme	single	1009:1014	arg1	content					1024:1030	total or single UA-GAGs content	1000:1030	content	1024:1030	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	7	12	theme	early	1307:1311	arg1	diagnosis					1313:1321	a possible early diagnosis	1296:1321	a possible early diagnosis of various MPS types	1296:1342	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	6	13	dep	CS	1060:1061	arg1	composition					1083:1093	disaccharide composition	1070:1093	disaccharide composition	1070:1093	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	5	14	theme	HS	825:826	arg1	evaluation					804:813	A chemical evaluation	793:813	A chemical evaluation of CS and HS structure	793:836	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	3	15	theme	uronic	457:462	arg1	acid					464:467	uronic acid	457:467	uronic acid (UA)-GAGs determination	457:491	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	15	theme	uronic	457:462	arg1	UA					470:471	UA	470:471	UA	470:471	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	16	theme	acid	464:467	arg1	determination					479:491	uronic acid (UA)-GAGs determination	457:491	uronic acid (UA)-GAGs determination	457:491	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	6	17	theme	content	1024:1030	arg1	signature					1115:1123	chemical signature	1106:1123	chemical signature	1106:1123	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	6	17	theme	content	1024:1030	arg1	increase					988:995	an increase	985:995	an increase of total or single UA-GAGs content	985:1030	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	3	18	theme	MPS	427:429	arg1	diagnosis					414:422	a possible early diagnosis	397:422	a possible early diagnosis of MPS	397:429	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	0	19	theme	mucopolysaccharidosis	102:122	arg1	diagnosis					89:97	the diagnosis	85:97	the diagnosis of mucopolysaccharidosis	85:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	6	20	theme	chemical	1106:1113	arg1	signature					1115:1123	chemical signature	1106:1123	chemical signature	1106:1123	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	6	21	theme	total	1000:1004	arg1	content					1024:1030	total or single UA-GAGs content	1000:1030	content	1024:1030	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	4	22	theme	heparan	679:685	arg1	HS					696:697	HS	696:697	HS	696:697	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	22	theme	heparan	679:685	arg1	sulfate					687:693	heparan sulfate	679:693	28.6% heparan sulfate (HS)	673:698	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	1	23	theme	cheap	164:168	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	23	theme	cheap	164:168	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	7	24	theme	possible	1298:1305	arg1	diagnosis					1313:1321	a possible early diagnosis	1296:1321	a possible early diagnosis of various MPS types	1296:1342	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	5	25	theme	chemical	795:802	arg1	evaluation					804:813	A chemical evaluation	793:813	A chemical evaluation of CS and HS structure	793:836	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	4	26	theme	normal	609:614	arg1	newborns					616:623	the normal newborns	605:623	the normal newborns	605:623	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	27	theme	chondroitin	647:657	arg1	CS					668:669	CS	668:669	CS	668:669	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	27	theme	chondroitin	647:657	arg1	sulfate					659:665	chondroitin sulfate	647:665	67.2% chondroitin sulfate (CS)	641:670	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	7	28	theme	types	1338:1342	arg1	diagnosis					1313:1321	a possible early diagnosis	1296:1321	a possible early diagnosis of various MPS types	1296:1342	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	29	theme	parameters	1213:1222	arg1	modifications					1171:1183	The modifications	1167:1183	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS	1167:1274	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	29	theme	parameters	1213:1222	arg1	useful					1285:1290	useful	1285:1290	useful	1285:1290	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	1	30	theme	easy	174:177	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	30	theme	easy	174:177	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	7	31	theme	newborns	1249:1256	arg1	structure					1228:1236	structure	1228:1236	structure	1228:1236	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	31	theme	newborns	1249:1256	arg1	parameters					1213:1222	parameters	1213:1222	parameters	1213:1222	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	31	theme	newborns	1249:1256	arg1	composition					1200:1210	UA-GAGs composition	1192:1210	UA-GAGs composition	1192:1210	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	32	theme	UA-GAGs	1192:1198	arg1	composition					1200:1210	UA-GAGs composition	1192:1210	UA-GAGs composition	1192:1210	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	4	33	theme	μg/DBS	785:790	arg1	content					769:775	a total GAGs content	756:775	a total GAGs content of 0.43 μg/DBS	756:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	33	theme	μg/DBS	785:790	arg1	2.35					747:750	2.35	747:750	2.35	747:750	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	34	theme	hyaluronic	709:718	arg1	acid					720:723	hyaluronic acid	709:723	4.4% hyaluronic acid	704:723	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	2	35	theme	enzymes	288:294	arg1	deficit					277:283	the deficit	273:283	the deficit of enzymes that degrade glycosaminoglycans (GAGs) characterized by progressive worsening of the conditions	273:390	Mucopolysaccharidoses (MPS) are characterized by the deficit of enzymes that degrade glycosaminoglycans (GAGs) characterized by progressive worsening of the conditions.
30009765	6	36	theme	types	969:973	arg1	DBS					943:945	The DBS	939:945	The DBS of four different MPS types	939:973	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	4	37	with	%	677:677	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	38	theme	0.43	780:783	arg1	μg/DBS					785:790	0.43 μg/DBS	780:790	0.43 μg/DBS	780:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	3	39	theme	newborns	515:522	arg1	DBS					496:498	DBS	496:498	DBS of 600 healthy newborns	496:522	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	1	40	theme	DBS	143:145	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	40	theme	DBS	143:145	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	3	41	theme	possible	399:406	arg1	diagnosis					414:422	a possible early diagnosis	397:422	a possible early diagnosis of MPS	397:429	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	6	42	theme	MPS	965:967	arg1	types					969:973	four different MPS types	950:973	four different MPS types	950:973	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	4	43	dep	%	707:707	arg1	acid					720:723	hyaluronic acid	709:723	4.4% hyaluronic acid	704:723	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	5	44	theme	disaccharide	871:882	arg1	composition					884:894	their disaccharide composition	865:894	their disaccharide composition	865:894	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	4	45	theme	blood	588:592	arg1	UA-GAGs					594:600	Spotted blood UA-GAGs	580:600	Spotted blood UA-GAGs of the normal newborns	580:623	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	0	46	theme	glycosaminoglycans	29:46	arg1	structure					16:24	structure	16:24	structure	16:24	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	0	46	theme	glycosaminoglycans	29:46	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	3	47	theme	early	408:412	arg1	diagnosis					414:422	a possible early diagnosis	397:422	a possible early diagnosis of MPS	397:429	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	0	48	from	newborns	72:79	arg1	structure					16:24	structure	16:24	structure	16:24	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	0	48	from	newborns	72:79	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	0	48	from	newborns	72:79	arg1	DBS					51:53	DBS	51:53	DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	51:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	7	49	theme	healthy	1241:1247	arg1	newborns					1249:1256	healthy newborns	1241:1256	healthy newborns determined in DBS	1241:1274	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	50	theme	structure	1228:1236	arg1	modifications					1171:1183	The modifications	1167:1183	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS	1167:1274	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	50	theme	structure	1228:1236	arg1	useful					1285:1290	useful	1285:1290	useful	1285:1290	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	4	51	theme	Spotted	580:586	arg1	UA-GAGs					594:600	Spotted blood UA-GAGs	580:600	Spotted blood UA-GAGs of the normal newborns	580:623	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	4	52	theme	GAGs	764:767	arg1	content					769:775	a total GAGs content	756:775	a total GAGs content of 0.43 μg/DBS	756:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	6	53	theme	disaccharide	1070:1081	arg1	composition					1083:1093	disaccharide composition	1070:1093	disaccharide composition	1070:1093	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	4	54	theme	CS/HS	732:736	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	1	55	theme	Dried	125:129	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	55	theme	Dried	125:129	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	7	56	theme	various	1326:1332	arg1	types					1338:1342	various MPS types	1326:1342	various MPS types	1326:1342	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	4	57	theme	total	758:762	arg1	content					769:775	a total GAGs content	756:775	a total GAGs content of 0.43 μg/DBS	756:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	3	58	theme	small	535:539	arg1	group					541:545	a small group	533:545	a small group of MPS subjects matched for age	533:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	58	theme	small	535:539	arg1	subjects					554:561	MPS subjects	550:561	MPS subjects matched for age	550:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	1	59	theme	blood	131:135	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	59	theme	blood	131:135	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	6	60	theme	HS	1067:1068	arg1	modifications					1039:1051	modifications	1039:1051	modifications of the CS and HS disaccharide composition	1039:1093	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	7	61	theme	composition	1200:1210	arg1	modifications					1171:1183	The modifications	1167:1183	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS	1167:1274	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	7	61	theme	composition	1200:1210	arg1	useful					1285:1290	useful	1285:1290	useful	1285:1290	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	6	62	theme	enzymatic	1149:1157	arg1	defect					1159:1164	the MPS enzymatic defect	1141:1164	the MPS enzymatic defect	1141:1164	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	3	63	theme	matched	563:569	arg1	subjects					554:561	MPS subjects	550:561	MPS subjects matched for age	550:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	4	64	theme	newborns	616:623	arg1	UA-GAGs					594:600	Spotted blood UA-GAGs	580:600	Spotted blood UA-GAGs of the normal newborns	580:623	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	1	65	theme	spot	137:140	arg1	technology					148:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology	125:157	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	1	65	theme	spot	137:140	arg1	method					179:184	a cheap and easy method	162:184	a cheap and easy method largely applied in newborn screening	162:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	3	66	theme	healthy	507:513	arg1	newborns					515:522	600 healthy newborns	503:522	600 healthy newborns	503:522	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	1	67	theme	newborn	205:211	arg1	screening					213:221	newborn screening	205:221	newborn screening	205:221	Dried blood spot (DBS) technology is a cheap and easy method largely applied in newborn screening.
30009765	4	68	theme	content	769:775	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	5	69	theme	overall	915:921	arg1	density					930:936	the overall charge density	911:936	the overall charge density	911:936	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	6	70	theme	CS	1060:1061	arg1	modifications					1039:1051	modifications	1039:1051	modifications of the CS and HS disaccharide composition	1039:1093	The DBS of four different MPS types presented an increase of total or single UA-GAGs content and/or modifications of the CS and HS disaccharide composition as well as chemical signature also related to the MPS enzymatic defect.
30009765	2	71	theme	progressive	352:362	arg1	worsening					364:372	progressive worsening	352:372	progressive worsening of the conditions	352:390	Mucopolysaccharidoses (MPS) are characterized by the deficit of enzymes that degrade glycosaminoglycans (GAGs) characterized by progressive worsening of the conditions.
30009765	5	72	theme	charge	923:928	arg1	density					930:936	the overall charge density	911:936	the overall charge density	911:936	A chemical evaluation of CS and HS structure was performed by measuring their disaccharide composition, sulfation and the overall charge density.
30009765	3	73	theme	MPS	550:552	arg1	subjects					554:561	MPS subjects	550:561	MPS subjects matched for age	550:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	74	theme	-GAGs	473:477	arg1	determination					479:491	uronic acid (UA)-GAGs determination	457:491	uronic acid (UA)-GAGs determination	457:491	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	2	75	theme	conditions	381:390	arg1	worsening					364:372	progressive worsening	352:372	progressive worsening of the conditions	352:390	Mucopolysaccharidoses (MPS) are characterized by the deficit of enzymes that degrade glycosaminoglycans (GAGs) characterized by progressive worsening of the conditions.
30009765	4	76	with	%	707:707	arg1	ratio					738:742	a CS/HS ratio	730:742	a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS	730:790	Spotted blood UA-GAGs of the normal newborns are composed of 67.2% chondroitin sulfate (CS), 28.6% heparan sulfate (HS) and 4.4% hyaluronic acid with a CS/HS ratio of 2.35 and a total GAGs content of 0.43 μg/DBS.
30009765	0	77	theme	2-3-day-old	60:70	arg1	newborns					72:79	2-3-day-old newborns	60:79	2-3-day-old newborns for the diagnosis of mucopolysaccharidosis	60:122	Composition and structure of glycosaminoglycans in DBS from 2-3-day-old newborns for the diagnosis of mucopolysaccharidosis.
30009765	7	78	theme	MPS	1334:1336	arg1	types					1338:1342	various MPS types	1326:1342	various MPS types	1326:1342	The modifications of the UA-GAGs composition, parameters and structure of healthy newborns determined in DBS would be useful for a possible early diagnosis of various MPS types.
30009765	3	79	theme	subjects	554:561	arg1	group					541:545	a small group	533:545	a small group of MPS subjects matched for age	533:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	79	theme	subjects	554:561	arg1	subjects					554:561	MPS subjects	550:561	MPS subjects matched for age	550:577	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30009765	3	80	theme	determination	479:491	arg1	method					447:452	a method	445:452	a method of uronic acid (UA)-GAGs determination	445:491	For a possible early diagnosis of MPS, we developed a method of uronic acid (UA)-GAGs determination in DBS of 600 healthy newborns and from a small group of MPS subjects matched for age.
30768248	0	0	theme	Bone	122:125	arg1	Regeneration					127:138	Bone Regeneration	122:138	Bone Regeneration	122:138	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	10	1	contain	has	1406:1408	arg2	potential					1419:1427	enormous potential	1410:1427	enormous potential for the treatment of bone diseases and prevention of locomotive syndrome	1410:1500	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	10	1	contain	has	1406:1408	arg1	bone					1337:1340	This injectable bone substitute	1321:1351	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity	1321:1404	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	7	2	theme	osteoclast	1072:1081	arg1	formation					1083:1091	osteoclast formation	1072:1091	osteoclast formation	1072:1091	pNC suppresses osteoclast formation and pit formation.
30768248	1	3	theme	invasive	185:192	arg1	bone					155:158	An injectable bone	141:158	An injectable bone	141:158	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	1	3	theme	invasive	185:192	arg1	therapy					194:200	a minimally invasive therapy	173:200	a minimally invasive therapy for large orthopedic defects and osteoporosis	173:246	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	5	4	with	responsiveness	911:924	arg1	β-TCP					852:856	β-TCP	852:856	β-TCP	852:856	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	1	5	theme	surgical	287:294	arg1	treatment					296:304	surgical treatment	287:304	surgical treatment	287:304	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	6	6	theme	pH	997:998	arg1	decrease					1000:1007	an osteoclast-induced pH decrease	975:1007	an osteoclast-induced pH decrease	975:1007	When the pH drops to ∼4.5, which corresponds to an osteoclast-induced pH decrease, pNC-β-TCP composite degrades and releases pNC.
30768248	8	7	theme	osteoclast-responsive	1117:1137	arg1	property					1139:1146	This osteoclast-responsive property	1112:1146	This osteoclast-responsive property	1112:1146	This osteoclast-responsive property allows for controlling the degradation rate of the composite.
30768248	6	8	theme	osteoclast-induced	978:995	arg1	decrease					1000:1007	an osteoclast-induced pH decrease	975:1007	an osteoclast-induced pH decrease	975:1007	When the pH drops to ∼4.5, which corresponds to an osteoclast-induced pH decrease, pNC-β-TCP composite degrades and releases pNC.
30768248	10	9	theme	pNC	1356:1358	arg1	bone					1337:1340	This injectable bone substitute	1321:1351	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity	1321:1404	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	9	10	theme	β-TCP	1278:1282	arg1	β-TCP					1278:1282	β-TCP	1278:1282	β-TCP	1278:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	10	theme	β-TCP	1278:1282	arg1	pNC					1237:1239	pNC	1237:1239	pNC	1237:1239	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	10	theme	β-TCP	1278:1282	arg1	phosphate					1255:1263	α-tricalcium phosphate	1242:1263	α-tricalcium phosphate (α-TCP)	1242:1271	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	10	theme	β-TCP	1278:1282	arg1	composite					1224:1232	the composite	1220:1232	the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP	1220:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	4	11	theme	β-tricalcium	666:677	arg1	β-TCP					690:694	β-TCP	690:694	β-TCP	690:694	Composites composed of nanofibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils.
30768248	4	11	theme	β-tricalcium	666:677	arg1	phosphate					679:687	β-tricalcium phosphate	666:687	β-tricalcium phosphate (β-TCP)	666:695	Composites composed of nanofibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils.
30768248	3	12	theme	bisphosphonate-modified	525:547	arg1	pNC					564:566	pNC	564:566	pNC	564:566	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	3	12	theme	bisphosphonate-modified	525:547	arg1	nanocellulose					549:561	bisphosphonate-modified nanocellulose	525:561	bisphosphonate-modified nanocellulose (pNC)	525:567	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	2	13	theme	conventional	316:327	arg1	bone					329:332	conventional bone	316:332	conventional bone	316:332	However, conventional bone substitutes lack the desirable biodegradability, bioresponsibility, and functionality to regulate the bone regeneration process.
30768248	1	14	theme	orthopedic	212:221	arg1	defects					223:229	large orthopedic defects	206:229	large orthopedic defects	206:229	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	5	15	theme	binding	878:884	arg1	property					886:893	binding property	878:893	binding property to bone	878:901	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	9	16	theme	osteoblast	1293:1302	arg1	differentiation					1304:1318	osteoblast differentiation	1293:1318	osteoblast differentiation	1293:1318	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	3	17	theme	bone	594:597	arg1	regeneration					599:610	bone regeneration	594:610	bone regeneration	594:610	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	1	18	theme	large	206:210	arg1	defects					223:229	large orthopedic defects	206:229	large orthopedic defects	206:229	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	2	19	theme	regeneration	441:452	arg1	process					454:460	the bone regeneration process	432:460	the bone regeneration process	432:460	However, conventional bone substitutes lack the desirable biodegradability, bioresponsibility, and functionality to regulate the bone regeneration process.
30768248	8	20	theme	degradation	1175:1185	arg1	rate					1187:1190	the degradation rate	1171:1190	the degradation rate of the composite	1171:1207	This osteoclast-responsive property allows for controlling the degradation rate of the composite.
30768248	2	21	theme	desirable	355:363	arg1	biodegradability					365:380	the desirable biodegradability	351:380	the desirable biodegradability	351:380	However, conventional bone substitutes lack the desirable biodegradability, bioresponsibility, and functionality to regulate the bone regeneration process.
30768248	3	22	theme	injectable	482:491	arg1	bone					508:511	an injectable, bioresponsive bone	479:511	an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration	479:610	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	5	23	theme	pH	908:909	arg1	responsiveness					911:924	pH responsiveness	908:924	pH responsiveness	908:924	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	3	24	dep	bone	574:577	arg1	substitute					579:588	substitute	579:588	substitute for bone regeneration	579:610	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	0	25	theme	Injectable	23:32	arg1	Osteoclast-Responsive					0:20	Osteoclast-Responsive	0:20	Osteoclast-Responsive	0:20	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	0	25	theme	Injectable	23:32	arg1	Bone					34:37	Injectable Bone	23:37	Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration	23:138	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	4	26	theme	nanofibrillated	636:650	arg1	cellulose					652:660	nanofibrillated cellulose	636:660	nanofibrillated cellulose	636:660	Composites composed of nanofibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils.
30768248	10	27	theme	diseases	1455:1462	arg1	prevention					1468:1477	prevention	1468:1477	prevention of locomotive syndrome	1468:1500	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	10	27	theme	diseases	1455:1462	arg1	treatment					1437:1445	the treatment	1433:1445	the treatment of bone diseases	1433:1462	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	1	28	theme	injectable	144:153	arg1	bone					155:158	An injectable bone	141:158	An injectable bone	141:158	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	1	28	theme	injectable	144:153	arg1	alternative					255:265	an alternative	252:265	an alternative to allografting and surgical treatment	252:304	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	1	28	theme	injectable	144:153	arg1	therapy					194:200	a minimally invasive therapy	173:200	a minimally invasive therapy for large orthopedic defects and osteoporosis	173:246	An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment.
30768248	4	29	theme	bone	703:706	arg1	structures					708:717	bone structures	703:717	bone structures in which apatite reinforces collagen fibrils	703:762	Composites composed of nanofibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils.
30768248	0	30	theme	Bisphosphonated-Nanocellulose	42:70	arg1	Osteoclast-Responsive					0:20	Osteoclast-Responsive	0:20	Osteoclast-Responsive	0:20	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	0	30	theme	Bisphosphonated-Nanocellulose	42:70	arg1	Bone					34:37	Injectable Bone	23:37	Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration	23:138	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	10	31	theme	enormous	1410:1417	arg1	potential					1419:1427	enormous potential	1410:1427	enormous potential for the treatment of bone diseases and prevention of locomotive syndrome	1410:1500	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	5	32	theme	reversible	812:821	arg1	cross-linking					833:845	reversible, physical cross-linking	812:845	reversible, physical cross-linking with β-TCP	812:856	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	2	33	theme	bone	436:439	arg1	process					454:460	the bone regeneration process	432:460	the bone regeneration process	432:460	However, conventional bone substitutes lack the desirable biodegradability, bioresponsibility, and functionality to regulate the bone regeneration process.
30768248	8	34	theme	composite	1199:1207	arg1	rate					1187:1190	the degradation rate	1171:1190	the degradation rate of the composite	1171:1207	This osteoclast-responsive property allows for controlling the degradation rate of the composite.
30768248	3	35	dep	injectable	482:491	arg1	bioresponsive					494:506	bioresponsive	494:506	bioresponsive	494:506	Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration.
30768248	5	36	theme	apatite	859:865	arg1	formation					867:875	apatite formation	859:875	apatite formation	859:875	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	9	37	theme	pNC	1237:1239	arg1	β-TCP					1278:1282	β-TCP	1278:1282	β-TCP	1278:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	37	theme	pNC	1237:1239	arg1	pNC					1237:1239	pNC	1237:1239	pNC	1237:1239	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	37	theme	pNC	1237:1239	arg1	phosphate					1255:1263	α-tricalcium phosphate	1242:1263	α-tricalcium phosphate (α-TCP)	1242:1271	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	37	theme	pNC	1237:1239	arg1	composite					1224:1232	the composite	1220:1232	the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP	1220:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	5	38	theme	Bisphosphonate	765:778	arg1	groups					780:785	Bisphosphonate groups	765:785	Bisphosphonate groups on nanocellulose	765:802	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	6	39	theme	pH	936:937	arg1	drops					939:943	the pH drops	932:943	the pH drops	932:943	When the pH drops to ∼4.5, which corresponds to an osteoclast-induced pH decrease, pNC-β-TCP composite degrades and releases pNC.
30768248	5	40	with	cross-linking	833:845	arg1	β-TCP					852:856	β-TCP	852:856	β-TCP	852:856	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	7	41	theme	pit	1097:1099	arg1	formation					1101:1109	pit formation	1097:1109	pit formation	1097:1109	pNC suppresses osteoclast formation and pit formation.
30768248	4	42	theme	collagen	747:754	arg1	fibrils					756:762	collagen fibrils	747:762	collagen fibrils	747:762	Composites composed of nanofibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils.
30768248	6	43	theme	pNC-β-TCP	1010:1018	arg1	composite					1020:1028	pNC-β-TCP composite	1010:1028	pNC-β-TCP composite	1010:1028	When the pH drops to ∼4.5, which corresponds to an osteoclast-induced pH decrease, pNC-β-TCP composite degrades and releases pNC.
30768248	10	44	dep	bone	1337:1340	arg1	substitute					1342:1351	substitute	1342:1351	substitute	1342:1351	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	5	45	with	formation	867:875	arg1	β-TCP					852:856	β-TCP	852:856	β-TCP	852:856	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	9	46	theme	α-tricalcium	1242:1253	arg1	α-TCP					1266:1270	α-TCP	1266:1270	α-TCP	1266:1270	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	46	theme	α-tricalcium	1242:1253	arg1	phosphate					1255:1263	α-tricalcium phosphate	1242:1263	α-tricalcium phosphate (α-TCP)	1242:1271	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	5	47	from	groups	780:785	arg1	nanocellulose					790:802	nanocellulose	790:802	nanocellulose	790:802	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	5	48	with	property	886:893	arg1	β-TCP					852:856	β-TCP	852:856	β-TCP	852:856	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	9	49	theme	phosphate	1255:1263	arg1	β-TCP					1278:1282	β-TCP	1278:1282	β-TCP	1278:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	49	theme	phosphate	1255:1263	arg1	pNC					1237:1239	pNC	1237:1239	pNC	1237:1239	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	49	theme	phosphate	1255:1263	arg1	phosphate					1255:1263	α-tricalcium phosphate	1242:1263	α-tricalcium phosphate (α-TCP)	1242:1271	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	9	49	theme	phosphate	1255:1263	arg1	composite					1224:1232	the composite	1220:1232	the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP	1220:1282	Moreover, the composite of pNC, α-tricalcium phosphate (α-TCP), and β-TCP enhances osteoblast differentiation.
30768248	10	50	theme	bone	1450:1453	arg1	diseases					1455:1462	bone diseases	1450:1462	bone diseases	1450:1462	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	5	51	dep	reversible	812:821	arg1	physical					824:831	physical	824:831	physical	824:831	Bisphosphonate groups on nanocellulose provide reversible, physical cross-linking with β-TCP, apatite formation, binding property to bone, and pH responsiveness.
30768248	10	52	theme	syndrome	1493:1500	arg1	prevention					1468:1477	prevention	1468:1477	prevention of locomotive syndrome	1468:1500	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	10	52	theme	syndrome	1493:1500	arg1	treatment					1437:1445	the treatment	1433:1445	the treatment of bone diseases	1433:1462	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	10	53	theme	injectable	1326:1335	arg1	bone					1337:1340	This injectable bone substitute	1321:1351	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity	1321:1404	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	0	54	theme	Osteoclast/Osteoblast	87:107	arg1	Activity					109:116	Osteoclast/Osteoblast Activity	87:116	Osteoclast/Osteoblast Activity for Bone Regeneration	87:138	Osteoclast-Responsive, Injectable Bone of Bisphosphonated-Nanocellulose that Regulates Osteoclast/Osteoblast Activity for Bone Regeneration.
30768248	10	55	theme	locomotive	1482:1491	arg1	syndrome					1493:1500	locomotive syndrome	1482:1500	locomotive syndrome	1482:1500	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
30768248	10	56	theme	osteoclast/osteoblast	1375:1395	arg1	activity					1397:1404	osteoclast/osteoblast activity	1375:1404	osteoclast/osteoblast activity	1375:1404	This injectable bone substitute of pNC that regulates osteoclast/osteoblast activity has enormous potential for the treatment of bone diseases and prevention of locomotive syndrome.
31426953	2	0	theme	Ca-alginate/cryoprotectants/cellulose	351:387	arg1	capsules					406:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	0	1	theme	release	85:91	arg1	behavior					93:100	controlled release behavior	74:100	controlled release behavior	74:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	4	2	theme	ACFP	748:751	arg1	capsules					753:760	The dry ACFP capsules	740:760	The dry ACFP capsules	740:760	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	6	3	theme	probiotic	1071:1079	arg1	cells					1081:1085	encapsulating probiotic cells	1057:1085	encapsulating probiotic cells	1057:1085	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	6	4	theme	cells	1081:1085	arg1	strategy					1045:1052	a strategy	1043:1052	a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime	1043:1211	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	0	5	from	Encapsulation	0:12	arg1	microgel					60:67	cellulose based microgel	44:67	cellulose based microgel with controlled release behavior	44:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	3	6	theme	protein	553:559	arg1	ingredients					575:585	whey protein isolate (WPI) ingredients	548:585	whey protein isolate (WPI) ingredients	548:585	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	3	7	dep	trehalose	534:542	arg1	The					530:532	The	530:532	The	530:532	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	6	8	theme	intestinal	1180:1189	arg1	fluid					1191:1195	simulated intestinal fluid	1170:1195	simulated intestinal fluid	1170:1195	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	3	9	theme	isolate	561:567	arg1	ingredients					575:585	whey protein isolate (WPI) ingredients	548:585	whey protein isolate (WPI) ingredients	548:585	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	1	10	theme	probiotic	268:276	arg1	products					278:285	the probiotic products	264:285	the probiotic products making function	264:301	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	0	11	theme	storage	126:132	arg1	stability					134:142	long-term storage stability	116:142	long-term storage stability	116:142	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	3	12	from	trehalose	534:542	arg1	cryoprotectants					594:608	the cryoprotectants	590:608	the cryoprotectants	590:608	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	5	13	theme	3.3 × 106 cfu/mL	956:971	arg1	3.3 × 106 cfu/mL					956:971	3.3 × 106 cfu/mL	956:971	3.3 × 106 cfu/mL	956:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	5	13	theme	3.3 × 106 cfu/mL	956:971	arg1	amount					946:951	the viable release amount	927:951	the viable release amount of 3.3 × 106 cfu/mL	927:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	6	14	theme	encapsulating	1057:1069	arg1	cells					1081:1085	encapsulating probiotic cells	1057:1085	encapsulating probiotic cells	1057:1085	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	0	15	theme	long-term	116:124	arg1	stability					134:142	long-term storage stability	116:142	long-term storage stability	116:142	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	2	16	theme	viability	479:487	arg1	loss					471:474	the minimal loss	459:474	the minimal loss of viability	459:487	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	3	17	theme	whey	548:551	arg1	ingredients					575:585	whey protein isolate (WPI) ingredients	548:585	whey protein isolate (WPI) ingredients	548:585	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	1	18	theme	human	192:196	arg1	health					198:203	human health	192:203	human health	192:203	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	3	19	theme	desired	724:730	arg1	region					732:737	the desired region	720:737	the desired region	720:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	0	20	theme	controlled	74:83	arg1	behavior					93:100	controlled release behavior	74:100	controlled release behavior	74:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	4	21	theme	dry	744:746	arg1	capsules					753:760	The dry ACFP capsules	740:760	The dry ACFP capsules	740:760	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	5	22	from	storage	983:989	arg1	4 °C					1006:1009	4 °C	1006:1009	4 °C	1006:1009	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	4	23	from	210 min	867:873	arg1	2.6 × 106 cfu/mL					847:862	2.6 × 106 cfu/mL	847:862	2.6 × 106 cfu/mL at 210 min	847:873	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	23	from	210 min	867:873	arg1	fluid					834:838	simulated intestinal fluid	813:838	simulated intestinal fluid (SIF)	813:844	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	3	24	from	release	700:706	arg1	region					732:737	the desired region	720:737	the desired region	720:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	6	25	theme	release	1150:1156	arg1	behavior					1158:1165	enhanced controlled release behavior	1130:1165	enhanced controlled release behavior	1130:1165	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	4	26	theme	gradual	772:778	arg1	release					780:786	gradual release	772:786	gradual release of L. plantarum cells	772:808	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	2	27	theme	ACFP	400:403	arg1	capsules					406:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	0	28	theme	plantarum	31:39	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior	0:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	6	29	theme	enhanced	1130:1137	arg1	behavior					1158:1165	enhanced controlled release behavior	1130:1165	enhanced controlled release behavior	1130:1165	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	5	30	theme	viable	931:936	arg1	3.3 × 106 cfu/mL					956:971	3.3 × 106 cfu/mL	956:971	3.3 × 106 cfu/mL	956:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	5	30	theme	viable	931:936	arg1	amount					946:951	the viable release amount	927:951	the viable release amount of 3.3 × 106 cfu/mL	927:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	5	31	theme	days	998:1001	arg1	storage					983:989	the storage	979:989	the storage of 160 days at 4 °C	979:1009	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	6	32	theme	long-term	1098:1106	arg1	stability					1116:1124	long-term storage stability	1098:1124	long-term storage stability	1098:1124	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	6	33	theme	simulated	1170:1178	arg1	fluid					1191:1195	simulated intestinal fluid	1170:1195	simulated intestinal fluid	1170:1195	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	3	34	theme	WPI	570:572	arg1	ingredients					575:585	whey protein isolate (WPI) ingredients	548:585	whey protein isolate (WPI) ingredients	548:585	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	2	35	theme	plantarum	429:437	arg1	cells					439:443	L. plantarum cells	426:443	L. plantarum cells	426:443	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	0	36	theme	cellulose	44:52	arg1	microgel					60:67	cellulose based microgel	44:67	cellulose based microgel with controlled release behavior	44:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	6	37	theme	promising	1016:1024	arg1	results					1026:1032	The promising results	1012:1032	The promising results	1012:1032	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	4	38	theme	intestinal	823:832	arg1	SIF					841:843	SIF	841:843	SIF	841:843	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	38	theme	intestinal	823:832	arg1	2.6 × 106 cfu/mL					847:862	2.6 × 106 cfu/mL	847:862	2.6 × 106 cfu/mL at 210 min	847:873	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	38	theme	intestinal	823:832	arg1	fluid					834:838	simulated intestinal fluid	813:838	simulated intestinal fluid (SIF)	813:844	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	5	39	from	4 °C	1006:1009	arg1	storage					983:989	the storage	979:989	the storage of 160 days at 4 °C	979:1009	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	5	39	from	4 °C	1006:1009	arg1	days					998:1001	160 days	994:1001	160 days at 4 °C	994:1009	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	2	40	theme	L.	426:427	arg1	cells					439:443	L. plantarum cells	426:443	L. plantarum cells	426:443	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	3	41	theme	Ca-alginate	644:654	arg1	shell					656:660	Ca-alginate shell	644:660	Ca-alginate shell	644:660	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	3	42	from	region	732:737	arg1	release					700:706	the release	696:706	the release of cells in the desired region	696:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	1	43	theme	good	217:220	arg1	stability					230:238	a good storage stability	215:238	a good storage stability	215:238	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	1	43	theme	good	217:220	arg1	prerequisite					247:258	the prerequisite	243:258	the prerequisite for the probiotic products making function	243:301	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	3	44	from	cells	711:715	arg1	region					732:737	the desired region	720:737	the desired region	720:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	3	45	theme	shell	656:660	arg1	time					636:639	the dissolution time	620:639	the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region	620:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	0	46	theme	based	54:58	arg1	microgel					60:67	cellulose based microgel	44:67	cellulose based microgel with controlled release behavior	44:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	2	47	theme	minimal	463:469	arg1	loss					471:474	the minimal loss	459:474	the minimal loss of viability	459:487	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	5	48	theme	dry	902:904	arg1	capsules					911:918	the dry ACFP capsules	898:918	the dry ACFP capsules	898:918	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	4	49	theme	cells	804:808	arg1	release					780:786	gradual release	772:786	gradual release of L. plantarum cells	772:808	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	50	theme	plantarum	794:802	arg1	cells					804:808	L. plantarum cells	791:808	L. plantarum cells	791:808	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	51	theme	simulated	813:821	arg1	SIF					841:843	SIF	841:843	SIF	841:843	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	51	theme	simulated	813:821	arg1	2.6 × 106 cfu/mL					847:862	2.6 × 106 cfu/mL	847:862	2.6 × 106 cfu/mL at 210 min	847:873	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	4	51	theme	simulated	813:821	arg1	fluid					834:838	simulated intestinal fluid	813:838	simulated intestinal fluid (SIF)	813:844	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	5	52	theme	ACFP	906:909	arg1	capsules					911:918	the dry ACFP capsules	898:918	the dry ACFP capsules	898:918	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	6	53	theme	controlled	1139:1148	arg1	behavior					1158:1165	enhanced controlled release behavior	1130:1165	enhanced controlled release behavior	1130:1165	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	1	54	theme	storage	222:228	arg1	stability					230:238	a good storage stability	215:238	a good storage stability	215:238	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	1	54	theme	storage	222:228	arg1	prerequisite					247:258	the prerequisite	243:258	the prerequisite for the probiotic products making function	243:301	Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function.
31426953	4	55	theme	L.	791:792	arg1	cells					804:808	L. plantarum cells	791:808	L. plantarum cells	791:808	The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 106 cfu/mL at 210 min.
31426953	0	56	with	microgel	60:67	arg1	behavior					93:100	controlled release behavior	74:100	controlled release behavior	74:100	Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.
31426953	5	57	theme	capsules	911:918	arg1	0.1 g					889:893	0.1 g	889:893	0.1 g of the dry ACFP capsules	889:918	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	6	58	theme	storage	1108:1114	arg1	stability					1116:1124	long-term storage stability	1098:1124	long-term storage stability	1098:1124	The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
31426953	2	59	theme	freeze-drying	507:519	arg1	process					521:527	the vacuum freeze-drying process	496:527	the vacuum freeze-drying process	496:527	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	5	60	theme	release	938:944	arg1	3.3 × 106 cfu/mL					956:971	3.3 × 106 cfu/mL	956:971	3.3 × 106 cfu/mL	956:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	5	60	theme	release	938:944	arg1	amount					946:951	the viable release amount	927:951	the viable release amount of 3.3 × 106 cfu/mL	927:971	In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 106 cfu/mL after the storage of 160 days at 4 °C.
31426953	3	61	from	ingredients	575:585	arg1	cryoprotectants					594:608	the cryoprotectants	590:608	the cryoprotectants	590:608	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	2	62	theme	vacuum	500:505	arg1	process					521:527	the vacuum freeze-drying process	496:527	the vacuum freeze-drying process	496:527	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31426953	3	63	theme	cells	711:715	arg1	release					700:706	the release	696:706	the release of cells in the desired region	696:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	3	64	theme	dissolution	624:634	arg1	time					636:639	the dissolution time	620:639	the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region	620:737	The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region.
31426953	2	65	theme	composite	389:397	arg1	capsules					406:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules	347:413	Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process.
31233794	8	0	theme	structural	1385:1394	arg1	changes					1396:1402	starch structural changes	1378:1402	starch structural changes of barley throughout malting and roasting	1378:1444	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	7	1	theme	starch	1255:1260	arg1	change					1271:1276	extremely low starch enthalpy change	1241:1276	extremely low starch enthalpy change	1241:1276	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	5	2	theme	amylose	1017:1023	arg1	chains					1025:1030	amylose chains	1017:1030	amylose chains followed by the reduction of gelatinization temperatures	1017:1087	After malting, significant degradation of amylose chains followed by the reduction of gelatinization temperatures was seen.
31233794	6	3	theme	malts	1116:1120	arg1	Roasting					1099:1106	Roasting	1099:1106	Roasting of pale malts	1099:1120	Roasting of pale malts was found to degrade starch and protein whilst completely stopping enzyme activities.
31233794	3	4	theme	gelatinization	789:802	arg1	temperatures					804:815	increased gelatinization temperatures	779:815	increased gelatinization temperatures (onset, peak, conclusion)	779:841	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	7	5	theme	enthalpy	1262:1269	arg1	change					1271:1276	extremely low starch enthalpy change	1241:1276	extremely low starch enthalpy change	1241:1276	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	3	6	theme	amylopectin	594:604	arg1	chains					606:611	Both amylose and amylopectin chains	577:611	Both amylose and amylopectin chains	577:611	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	2	7	theme	Starch	314:319	arg1	transitions					329:339	Starch thermal transitions	314:339	Starch thermal transitions	314:339	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	2	8	theme	thermal	321:327	arg1	transitions					329:339	Starch thermal transitions	314:339	Starch thermal transitions	314:339	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	1	9	theme	starch	286:291	arg1	structure					227:235	molecular structure	217:235	molecular structure	217:235	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	9	theme	starch	286:291	arg1	compositions					247:258	chemical compositions	238:258	chemical compositions	238:258	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	9	theme	starch	286:291	arg1	properties					272:281	thermal properties	264:281	thermal properties	264:281	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	7	10	theme	low	1251:1253	arg1	change					1271:1276	extremely low starch enthalpy change	1241:1276	extremely low starch enthalpy change	1241:1276	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	8	11	theme	fermentable	1481:1491	arg1	production					1499:1508	fermentable sugar production	1481:1508	fermentable sugar production during mashing	1481:1523	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	6	12	theme	pale	1111:1114	arg1	malts					1116:1120	pale malts	1111:1120	pale malts	1111:1120	Roasting of pale malts was found to degrade starch and protein whilst completely stopping enzyme activities.
31233794	4	13	theme	starch	959:964	arg1	content					966:972	decreased starch content	949:972	decreased starch content	949:972	Amylolytic enzyme activities increased during germination, resulting in decreased starch content.
31233794	4	14	theme	Amylolytic	877:886	arg1	activities					895:904	Amylolytic enzyme activities	877:904	Amylolytic enzyme activities	877:904	Amylolytic enzyme activities increased during germination, resulting in decreased starch content.
31233794	1	15	theme	underlying	171:180	arg1	mechanisms					182:191	the underlying mechanisms	167:191	the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process	167:311	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	7	16	theme	high	1326:1329	arg1	temperature					1331:1341	high temperature	1326:1341	high temperature	1326:1341	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	5	17	theme	chains	1025:1030	arg1	degradation					1002:1012	significant degradation	990:1012	significant degradation of amylose chains followed by the reduction of gelatinization temperatures	990:1087	After malting, significant degradation of amylose chains followed by the reduction of gelatinization temperatures was seen.
31233794	4	18	theme	decreased	949:957	arg1	content					966:972	decreased starch content	949:972	decreased starch content	949:972	Amylolytic enzyme activities increased during germination, resulting in decreased starch content.
31233794	7	19	theme	crystallinity	1309:1321	arg1	loss					1289:1292	the loss	1285:1292	the loss of amylopectin crystallinity at high temperature	1285:1341	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	7	20	theme	amylopectin	1297:1307	arg1	crystallinity					1309:1321	amylopectin crystallinity	1297:1321	amylopectin crystallinity	1297:1321	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	3	21	from	reduction	847:855	arg1	change					869:874	enthalpy change	860:874	enthalpy change	860:874	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	22	theme	preferential	655:666	arg1	attack					668:673	a preferential attack	653:673	a preferential attack of amylopectin chains	653:695	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	0	23	theme	Starch	0:5	arg1	structure-property					7:24	Starch structure-property	0:24	Starch structure-property	0:24	Starch structure-property relations as a function of barley germination times.
31233794	8	24	theme	determining	1457:1467	arg1	factors					1469:1475	determining factors	1457:1475	determining factors for fermentable sugar production during mashing	1457:1523	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	8	24	theme	determining	1457:1467	arg1	insights					1364:1371	insights	1364:1371	insights	1364:1371	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	7	25	theme	coloured	1222:1229	arg1	malts					1231:1235	The resulting coloured malts	1208:1235	The resulting coloured malts	1208:1235	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	5	26	theme	gelatinization	1061:1074	arg1	temperatures					1076:1087	gelatinization temperatures	1061:1087	gelatinization temperatures	1061:1087	After malting, significant degradation of amylose chains followed by the reduction of gelatinization temperatures was seen.
31233794	2	27	theme	fine	414:417	arg1	structure					419:427	the molecular fine structure	400:427	the molecular fine structure of amylose and amylopectin	400:454	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	2	28	theme	size-exclusion	478:491	arg1	chromatography					493:506	size-exclusion chromatography	478:506	size-exclusion chromatography	478:506	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	3	29	dep	temperatures	804:815	arg1	peak					825:828	peak	825:828	peak	825:828	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	29	dep	temperatures	804:815	arg1	onset					818:822	onset	818:822	onset	818:822	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	29	dep	temperatures	804:815	arg1	conclusion					831:840	conclusion	831:840	conclusion	831:840	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	1	30	theme	barley	96:101	arg1	samples					103:109	barley samples	96:109	barley samples	96:109	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	8	31	theme	barley	1407:1412	arg1	changes					1396:1402	starch structural changes	1378:1402	starch structural changes of barley throughout malting and roasting	1378:1444	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	3	32	theme	amylose	749:755	arg1	content					757:763	relative amylose content	740:763	relative amylose content	740:763	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	1	33	theme	samples	103:109	arg1	varieties					83:91	Two varieties	79:91	Two varieties of barley samples	79:109	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	33	theme	samples	103:109	arg1	samples					103:109	barley samples	96:109	barley samples	96:109	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	34	theme	molecular	217:225	arg1	structure					227:235	molecular structure	217:235	molecular structure	217:235	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	3	35	theme	amylose	582:588	arg1	chains					606:611	Both amylose and amylopectin chains	577:611	Both amylose and amylopectin chains	577:611	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	36	theme	content	757:763	arg1	increases					727:735	concomitant increases	715:735	concomitant increases of relative amylose content	715:763	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	37	theme	chains	690:695	arg1	attack					668:673	a preferential attack	653:673	a preferential attack of amylopectin chains	653:695	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	5	38	theme	significant	990:1000	arg1	degradation					1002:1012	significant degradation	990:1012	significant degradation of amylose chains followed by the reduction of gelatinization temperatures	990:1087	After malting, significant degradation of amylose chains followed by the reduction of gelatinization temperatures was seen.
31233794	2	39	theme	amylopectin	444:454	arg1	structure					419:427	the molecular fine structure	400:427	the molecular fine structure of amylose and amylopectin	400:454	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	2	40	theme	amylose	432:438	arg1	structure					419:427	the molecular fine structure	400:427	the molecular fine structure of amylose and amylopectin	400:454	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	3	41	from	temperatures	804:815	arg1	change					869:874	enthalpy change	860:874	enthalpy change	860:874	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	7	42	from	temperature	1331:1341	arg1	loss					1289:1292	the loss	1285:1292	the loss of amylopectin crystallinity at high temperature	1285:1341	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	2	43	theme	carbohydrate	533:544	arg1	electrophoresis					546:560	fluorophore-assisted carbohydrate electrophoresis	512:560	fluorophore-assisted carbohydrate electrophoresis	512:560	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	8	44	theme	sugar	1493:1497	arg1	production					1499:1508	fermentable sugar production	1481:1508	fermentable sugar production during mashing	1481:1523	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	0	45	theme	germination	60:70	arg1	times					72:76	barley germination times	53:76	barley germination times	53:76	Starch structure-property relations as a function of barley germination times.
31233794	2	46	theme	fluorophore-assisted	512:531	arg1	electrophoresis					546:560	fluorophore-assisted carbohydrate electrophoresis	512:560	fluorophore-assisted carbohydrate electrophoresis	512:560	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	3	47	theme	enthalpy	860:867	arg1	change					869:874	enthalpy change	860:874	enthalpy change	860:874	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	6	48	theme	enzyme	1189:1194	arg1	activities					1196:1205	enzyme activities	1189:1205	enzyme activities	1189:1205	Roasting of pale malts was found to degrade starch and protein whilst completely stopping enzyme activities.
31233794	3	49	theme	relative	740:747	arg1	content					757:763	relative amylose content	740:763	relative amylose content	740:763	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	50	theme	amylopectin	678:688	arg1	chains					690:695	amylopectin chains	678:695	amylopectin chains	678:695	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	2	51	theme	scanning	374:381	arg1	calorimetry					383:393	differential scanning calorimetry	361:393	differential scanning calorimetry	361:393	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	3	52	theme	increased	779:787	arg1	temperatures					804:815	increased gelatinization temperatures	779:815	increased gelatinization temperatures (onset, peak, conclusion)	779:841	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	3	53	theme	concomitant	715:725	arg1	increases					727:735	concomitant increases	715:735	concomitant increases of relative amylose content	715:763	Both amylose and amylopectin chains were hydrolyzed during germination, but a preferential attack of amylopectin chains was observed with concomitant increases of relative amylose content, resulting in increased gelatinization temperatures (onset, peak, conclusion) and reduction in enthalpy change.
31233794	2	54	theme	differential	361:372	arg1	calorimetry					383:393	differential scanning calorimetry	361:393	differential scanning calorimetry	361:393	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	5	55	theme	temperatures	1076:1087	arg1	reduction					1048:1056	the reduction	1044:1056	the reduction of gelatinization temperatures	1044:1087	After malting, significant degradation of amylose chains followed by the reduction of gelatinization temperatures was seen.
31233794	1	56	theme	chemical	238:245	arg1	compositions					247:258	chemical compositions	238:258	chemical compositions	238:258	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	4	57	theme	enzyme	888:893	arg1	activities					895:904	Amylolytic enzyme activities	877:904	Amylolytic enzyme activities	877:904	Amylolytic enzyme activities increased during germination, resulting in decreased starch content.
31233794	1	58	theme	germination	129:139	arg1	conditions					141:150	germination conditions	129:150	germination conditions	129:150	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	7	59	contain	had	1237:1239	arg1	malts					1231:1235	The resulting coloured malts	1208:1235	The resulting coloured malts	1208:1235	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	7	59	contain	had	1237:1239	arg2	change					1271:1276	extremely low starch enthalpy change	1241:1276	extremely low starch enthalpy change	1241:1276	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	7	60	theme	resulting	1212:1220	arg1	malts					1231:1235	The resulting coloured malts	1208:1235	The resulting coloured malts	1208:1235	The resulting coloured malts had extremely low starch enthalpy change due to the loss of amylopectin crystallinity at high temperature.
31233794	0	61	theme	times	72:76	arg1	function					41:48	a function	39:48	a function of barley germination times	39:76	Starch structure-property relations as a function of barley germination times.
31233794	1	62	from	changes	206:212	arg1	structure					227:235	molecular structure	217:235	molecular structure	217:235	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	62	from	changes	206:212	arg1	compositions					247:258	chemical compositions	238:258	chemical compositions	238:258	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	1	62	from	changes	206:212	arg1	properties					272:281	thermal properties	264:281	thermal properties	264:281	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31233794	2	63	theme	molecular	404:412	arg1	structure					419:427	the molecular fine structure	400:427	the molecular fine structure of amylose and amylopectin	400:454	Starch thermal transitions were examined using differential scanning calorimetry, and the molecular fine structure of amylose and amylopectin were determined using size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively.
31233794	0	64	theme	barley	53:58	arg1	times					72:76	barley germination times	53:76	barley germination times	53:76	Starch structure-property relations as a function of barley germination times.
31233794	8	65	theme	starch	1378:1383	arg1	changes					1396:1402	starch structural changes	1378:1402	starch structural changes of barley throughout malting and roasting	1378:1444	This study provides insights into starch structural changes of barley throughout malting and roasting, which are determining factors for fermentable sugar production during mashing.
31233794	1	66	theme	thermal	264:270	arg1	properties					272:281	thermal properties	264:281	thermal properties	264:281	Two varieties of barley samples were subjected to germination conditions to investigate the underlying mechanisms underpinning changes in molecular structure, chemical compositions and thermal properties of starch during this process.
31732053	9	0	theme	PP	1330:1331	arg1	Oryza					1431:1435	Oryza	1431:1435	Oryza	1431:1435	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	9	0	theme	PP	1330:1331	arg1	identity					1304:1311	the identity	1300:1311	the identity of 24 and 23 kDa PP, respectively	1300:1345	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	9	0	theme	PP	1330:1331	arg1	protein					1387:1393	pathogenesis-related protein 1	1366:1395	pathogenesis-related protein 1 (Oryza sativa Japonica Group)	1366:1425	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	0	1	theme	composition	116:126	arg1	role					89:92	the role	85:92	the role of starch and protein composition to head rice recovery	85:148	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	0	2	theme	rice	136:139	arg1	recovery					141:148	head rice recovery	131:148	head rice recovery	131:148	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	4	3	from	decrease	564:571	arg1	protein					576:582	protein	576:582	protein	576:582	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	1	4	theme	variable	219:226	arg1	degree					228:233	variable degree	219:233	variable degree of milling (DoM)	219:250	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	11	5	theme	protein	1786:1792	arg1	filling					1794:1800	abnormal protein filling	1777:1800	abnormal protein filling	1777:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	10	6	theme	inter-cultivar	1618:1631	arg1	differences					1633:1643	the inter-cultivar differences	1614:1643	the inter-cultivar differences in the starch structure and packaging	1614:1681	However, the inter-cultivar differences in the starch structure and packaging were also observed.
31732053	11	7	theme	cultivars	1876:1884	arg1	BR					1860:1861	BR	1860:1861	BR of different cultivars	1860:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	9	8	theme	proteins	1588:1595	arg1	matrix					1597:1602	the proteins matrix	1584:1602	the proteins matrix	1584:1602	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	4	9	theme	extended	542:549	arg1	DoM					551:553	The extended DoM	538:553	The extended DoM	538:553	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	3	10	theme	Extended	438:445	arg1	DoM					447:449	Extended DoM	438:449	Extended DoM of brown rice	438:463	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	9	11	theme	pathogenesis-related	1366:1385	arg1	identity					1304:1311	the identity	1300:1311	the identity of 24 and 23 kDa PP, respectively	1300:1345	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	9	11	theme	pathogenesis-related	1366:1385	arg1	protein					1387:1393	pathogenesis-related protein 1	1366:1395	pathogenesis-related protein 1 (Oryza sativa Japonica Group)	1366:1425	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	1	12	theme	milling	238:244	arg1	degree					228:233	variable degree	219:233	variable degree of milling (DoM)	219:250	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	10	13	theme	starch	1652:1657	arg1	structure					1659:1667	the starch structure	1648:1667	the starch structure	1648:1667	However, the inter-cultivar differences in the starch structure and packaging were also observed.
31732053	1	14	from	difference	263:272	arg1	head					318:321	head	318:321	head (HR)	318:326	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	14	from	difference	263:272	arg1	rice					339:342	broken rice	332:342	broken rice (BR)	332:347	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	14	from	difference	263:272	arg1	BR					345:346	BR	345:346	BR	345:346	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	14	from	difference	263:272	arg1	proteins					277:284	proteins	277:284	proteins	277:284	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	14	from	difference	263:272	arg1	HR					324:325	HR	324:325	HR	324:325	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	14	from	difference	263:272	arg1	starches					290:297	starches	290:297	starches	290:297	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	7	15	theme	quantitative	1102:1113	arg1	changes					1115:1121	The major quantitative changes	1092:1121	The major quantitative changes	1092:1121	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	11	16	from	cracks	1827:1832	arg1	granules					1848:1855	the starch granules	1837:1855	the starch granules of BR of different cultivars	1837:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	3	17	theme	rice	460:463	arg1	DoM					447:449	Extended DoM	438:449	Extended DoM of brown rice	438:463	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	0	18	theme	starch	97:102	arg1	role					89:92	the role	85:92	the role of starch and protein composition to head rice recovery	85:148	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	1	19	theme	long-grain	175:184	arg1	cultivars					193:201	different long-grain Indica cultivars	165:201	different long-grain Indica cultivars	165:201	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	6	20	theme	12 kDa	983:988	arg1	PPs					990:992	12 kDa PPs	983:992	12 kDa PPs	983:992	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	4	21	theme	fat	588:590	arg1	content					592:598	fat content	588:598	fat content	588:598	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	0	22	theme	protein	108:114	arg1	composition					116:126	protein composition	108:126	protein composition	108:126	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	3	23	theme	progressive	479:489	arg1	decrease					491:498	a progressive decrease	477:498	a progressive decrease in HR yield	477:510	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	11	24	theme	starch	1755:1760	arg1	particles					1762:1770	starch particles	1755:1770	starch particles with abnormal protein filling	1755:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	5	25	theme	rice	757:760	arg1	cultivars					762:770	different rice cultivars	747:770	different rice cultivars	747:770	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	0	26	theme	grain	43:47	arg1	cultivars					61:69	long grain Indica rice cultivars	38:69	long grain Indica rice cultivars	38:69	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	11	27	theme	starch	1841:1846	arg1	granules					1848:1855	the starch granules	1837:1855	the starch granules of BR of different cultivars	1837:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	6	28	theme	different	835:843	arg1	cultivars					845:853	different cultivars	835:853	different cultivars	835:853	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	9	29	theme	1-Cys	1444:1448	arg1	HR					1467:1468	1-Cys peroxiredoxin A. HR	1444:1468	1-Cys peroxiredoxin A. HR	1444:1468	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	6	30	from	cultivars	845:853	arg1	rice					825:828	Milled rice	818:828	Milled rice from different cultivars	818:853	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	0	31	theme	rice	56:59	arg1	cultivars					61:69	long grain Indica rice cultivars	38:69	long grain Indica rice cultivars	38:69	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	10	32	from	differences	1633:1643	arg1	packaging					1673:1681	packaging	1673:1681	packaging	1673:1681	However, the inter-cultivar differences in the starch structure and packaging were also observed.
31732053	10	32	from	differences	1633:1643	arg1	structure					1659:1667	the starch structure	1648:1667	the starch structure	1648:1667	However, the inter-cultivar differences in the starch structure and packaging were also observed.
31732053	9	33	theme	A.	1464:1465	arg1	HR					1467:1468	1-Cys peroxiredoxin A. HR	1444:1468	1-Cys peroxiredoxin A. HR	1444:1468	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	11	34	from	accumulation	1739:1750	arg1	granules					1848:1855	the starch granules	1837:1855	the starch granules of BR of different cultivars	1837:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	2	35	from	accumulation	378:389	arg1	HR					427:428	HR	427:428	HR	427:428	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
31732053	2	35	from	accumulation	378:389	arg1	BR					434:435	BR	434:435	BR	434:435	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
31732053	6	36	dep	showed	872:877	arg1	least					1063:1067	least	1063:1067	least	1063:1067	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	11	37	theme	different	1866:1874	arg1	cultivars					1876:1884	different cultivars	1866:1884	different cultivars	1866:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	11	38	with	particles	1762:1770	arg1	filling					1794:1800	abnormal protein filling	1777:1800	abnormal protein filling	1777:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	0	39	theme	broken	23:28	arg1	rice					30:33	broken rice	23:33	broken rice	23:33	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	8	40	theme	60S	1232:1234	arg1	protein					1246:1252	60S ribosomal protein	1232:1252	60S ribosomal protein L10a	1232:1257	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	9	41	dep	Oryza	1398:1402	arg1	Group					1420:1424	Group	1420:1424	Oryza sativa Japonica Group	1398:1424	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	9	41	dep	Oryza	1398:1402	arg1	Japonica					1411:1418	Oryza sativa Japonica	1398:1418	Oryza sativa Japonica Group	1398:1424	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	3	42	from	increase	516:523	arg1	yield					531:535	BR yield	528:535	BR yield	528:535	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	3	42	from	increase	516:523	arg1	yield					506:510	HR yield	503:510	HR yield	503:510	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	7	43	located	observed	1128:1135	arg1	28 kDa					1140:1145	28 kDa	1140:1145	28 kDa	1140:1145	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	7	43	located	observed	1128:1135	arg1	PPs					1167:1169	23 kDa PPs	1160:1169	23 kDa PPs	1160:1169	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	7	43	located	observed	1128:1135	arg2	changes					1115:1121	The major quantitative changes	1092:1121	The major quantitative changes	1092:1121	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	7	43	located	observed	1128:1135	arg1	24 kDa					1148:1153	24 kDa	1148:1153	24 kDa	1148:1153	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	6	44	theme	higher	879:884	arg1	levels					886:891	higher levels	879:891	higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs	879:1053	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	8	45	theme	protein	1246:1252	arg1	L10a					1254:1257	60S ribosomal protein L10a	1232:1257	60S ribosomal protein L10a	1232:1257	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	0	46	theme	head	14:17	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.	0:149	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	9	47	theme	packaged	1510:1517	arg1	granules					1526:1533	highly condensed packaged starch granules	1493:1533	highly condensed packaged starch granules	1493:1533	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	1	48	dep	proteins	277:284	arg1	characteristics					299:313	characteristics	299:313	characteristics	299:313	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	4	49	theme	final	662:666	arg1	viscosity					668:676	final viscosity	662:676	final viscosity	662:676	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	9	50	dep	protein	1387:1393	arg1	Oryza					1398:1402	Oryza	1398:1402	Oryza	1398:1402	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	2	51	theme	starch	394:399	arg1	accumulation					378:389	differential accumulation	365:389	differential accumulation of starch, fat and proteins in both HR and BR	365:435	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
31732053	9	52	theme	granules	1526:1533	arg1	presence					1481:1488	the presence	1477:1488	the presence of highly condensed packaged starch granules	1477:1533	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	8	53	theme	type-D	1272:1277	arg1	proteins					1281:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	8	53	theme	type-D	1272:1277	arg1	identity					1207:1214	the identity	1203:1214	the identity of 28 kDa PP	1203:1227	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	3	54	theme	BR	528:529	arg1	yield					531:535	BR yield	528:535	BR yield	528:535	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	9	55	theme	smooth	1540:1545	arg1	edges					1547:1551	smooth edges	1540:1551	smooth edges	1540:1551	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	4	56	from	content	592:598	arg1	protein					576:582	protein	576:582	protein	576:582	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	1	57	theme	Indica	186:191	arg1	cultivars					193:201	different long-grain Indica cultivars	165:201	different long-grain Indica cultivars	165:201	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	11	58	theme	BR	1860:1861	arg1	granules					1848:1855	the starch granules	1837:1855	the starch granules of BR of different cultivars	1837:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	0	59	dep	Evaluation	0:9	arg1	Evidence					72:79	Evidence	72:79	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.	0:149	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	11	60	theme	several	1806:1812	arg1	fissures					1814:1821	several fissures	1806:1821	several fissures	1806:1821	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	7	61	theme	23 kDa	1160:1165	arg1	PPs					1167:1169	23 kDa PPs	1160:1169	23 kDa PPs	1160:1169	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	2	62	theme	fat	402:404	arg1	accumulation					378:389	differential accumulation	365:389	differential accumulation of starch, fat and proteins in both HR and BR	365:435	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
31732053	11	63	from	fissures	1814:1821	arg1	granules					1848:1855	the starch granules	1837:1855	the starch granules of BR of different cultivars	1837:1884	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	3	64	theme	brown	454:458	arg1	rice					460:463	brown rice	454:463	brown rice	454:463	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	9	65	dep	Oryza	1431:1435	arg1	showed					1470:1475	showed	1470:1475	showed the presence of highly condensed packaged starch granules with smooth edges	1470:1551	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	9	65	dep	Oryza	1431:1435	arg1	sativa					1437:1442	Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges	1431:1551	Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges	1431:1551	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	1	66	theme	Brown	151:155	arg1	rice					157:160	Brown rice	151:160	Brown rice of different long-grain Indica cultivars	151:201	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	67	theme	different	165:173	arg1	cultivars					193:201	different long-grain Indica cultivars	165:201	different long-grain Indica cultivars	165:201	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	11	68	theme	lesser	1732:1737	arg1	accumulation					1739:1750	lesser accumulation	1732:1750	lesser accumulation of starch particles with abnormal protein filling	1732:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	7	69	theme	major	1096:1100	arg1	changes					1115:1121	The major quantitative changes	1092:1121	The major quantitative changes	1092:1121	The major quantitative changes were observed in 28 kDa, 24 kDa, and 23 kDa PPs.
31732053	6	70	theme	12 kDa	1044:1049	arg1	PPs					1051:1053	12 kDa PPs	1044:1053	12 kDa PPs	1044:1053	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	1	71	theme	broken	332:337	arg1	BR					345:346	BR	345:346	BR	345:346	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	1	71	theme	broken	332:337	arg1	rice					339:342	broken rice	332:342	broken rice (BR)	332:347	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	5	72	theme	BR	741:742	arg1	viscosity					721:729	the setback viscosity	709:729	the setback viscosity of HR and BR of different rice cultivars	709:770	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	11	73	theme	abnormal	1777:1784	arg1	filling					1794:1800	abnormal protein filling	1777:1800	abnormal protein filling	1777:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	8	74	theme	MALDI-ToF/MS	1172:1183	arg1	analysis					1185:1192	MALDI-ToF/MS analysis	1172:1192	MALDI-ToF/MS analysis	1172:1192	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	0	75	theme	head	131:134	arg1	recovery					141:148	head rice recovery	131:148	head rice recovery	131:148	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	5	76	theme	different	747:755	arg1	cultivars					762:770	different rice cultivars	747:770	different rice cultivars	747:770	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	5	77	theme	setback	713:719	arg1	viscosity					721:729	the setback viscosity	709:729	the setback viscosity of HR and BR of different rice cultivars	709:770	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	11	78	theme	particles	1762:1770	arg1	accumulation					1739:1750	lesser accumulation	1732:1750	lesser accumulation of starch particles with abnormal protein filling	1732:1800	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	11	78	theme	particles	1762:1770	arg1	cracks					1827:1832	cracks	1827:1832	cracks	1827:1832	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	11	78	theme	particles	1762:1770	arg1	fissures					1814:1821	several fissures	1806:1821	several fissures	1806:1821	On the contrary, BR revealed lesser accumulation of starch particles with abnormal protein filling and several fissures and cracks in the starch granules of BR of different cultivars.
31732053	0	79	theme	Indica	49:54	arg1	cultivars					61:69	long grain Indica rice cultivars	38:69	long grain Indica rice cultivars	38:69	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	3	80	theme	HR	503:504	arg1	yield					506:510	HR yield	503:510	HR yield	503:510	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	2	81	theme	proteins	410:417	arg1	accumulation					378:389	differential accumulation	365:389	differential accumulation of starch, fat and proteins in both HR and BR	365:435	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
31732053	0	82	theme	cultivars	61:69	arg1	head					14:17	head	14:17	head	14:17	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	0	82	theme	cultivars	61:69	arg1	rice					30:33	broken rice	23:33	broken rice	23:33	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	4	83	from	increase	631:638	arg1	viscosity					648:656	peak viscosity	643:656	peak viscosity	643:656	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	4	83	from	increase	631:638	arg1	viscosity					668:676	final viscosity	662:676	final viscosity	662:676	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	5	84	theme	HR	734:735	arg1	viscosity					721:729	the setback viscosity	709:729	the setback viscosity of HR and BR of different rice cultivars	709:770	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	9	85	theme	peroxiredoxin	1450:1462	arg1	HR					1467:1468	1-Cys peroxiredoxin A. HR	1444:1468	1-Cys peroxiredoxin A. HR	1444:1468	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	8	86	theme	28 kDa	1219:1224	arg1	PP					1226:1227	28 kDa PP	1219:1227	28 kDa PP	1219:1227	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	6	87	theme	Milled	818:823	arg1	rice					825:828	Milled rice	818:828	Milled rice from different cultivars	818:853	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	6	88	theme	59 kDa	896:901	arg1	levels					886:891	higher levels	879:891	higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs	879:1053	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	8	89	theme	ribosomal	1236:1244	arg1	protein					1246:1252	60S ribosomal protein	1232:1252	60S ribosomal protein L10a	1232:1257	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	5	90	theme	cultivars	762:770	arg1	HR					734:735	HR	734:735	HR	734:735	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	5	90	theme	cultivars	762:770	arg1	BR					741:742	BR	741:742	BR	741:742	On the contrary, the setback viscosity of HR and BR of different rice cultivars was influenced by cultivars and extended DoM.
31732053	8	91	theme	PP	1226:1227	arg1	proteins					1281:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	8	91	theme	PP	1226:1227	arg1	identity					1207:1214	the identity	1203:1214	the identity of 28 kDa PP	1203:1227	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	8	92	theme	L10a	1254:1257	arg1	proteins					1281:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	60S ribosomal protein L10a and glutelin type-D 1 proteins	1232:1288	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	8	92	theme	L10a	1254:1257	arg1	identity					1207:1214	the identity	1203:1214	the identity of 28 kDa PP	1203:1227	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	9	93	theme	condensed	1500:1508	arg1	granules					1526:1533	highly condensed packaged starch granules	1493:1533	highly condensed packaged starch granules	1493:1533	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	8	94	theme	glutelin	1263:1270	arg1	type-D					1272:1277	glutelin type-D 1	1263:1279	glutelin type-D 1	1263:1279	MALDI-ToF/MS analysis revealed the identity of 28 kDa PP as 60S ribosomal protein L10a and glutelin type-D 1 proteins.
31732053	3	95	from	decrease	491:498	arg1	yield					531:535	BR yield	528:535	BR yield	528:535	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	3	95	from	decrease	491:498	arg1	yield					506:510	HR yield	503:510	HR yield	503:510	Extended DoM of brown rice resulted in a progressive decrease in HR yield and increase in BR yield.
31732053	0	96	theme	rice	30:33	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.	0:149	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	6	97	theme	%	866:866	arg1	DoM					868:870	6% DoM	865:870	6% DoM	865:870	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	9	98	theme	starch	1519:1524	arg1	granules					1526:1533	highly condensed packaged starch granules	1493:1533	highly condensed packaged starch granules	1493:1533	Whereas, the identity of 24 and 23 kDa PP, respectively was established as pathogenesis-related protein 1 (Oryza sativa Japonica Group) and Oryza sativa 1-Cys peroxiredoxin A. HR showed the presence of highly condensed packaged starch granules with smooth edges, which were tightly imbibed in the proteins matrix.
31732053	0	99	theme	long	38:41	arg1	cultivars					61:69	long grain Indica rice cultivars	38:69	long grain Indica rice cultivars	38:69	Evaluation of head and broken rice of long grain Indica rice cultivars: Evidence for the role of starch and protein composition to head rice recovery.
31732053	6	100	theme	PPs	1051:1053	arg1	levels					886:891	higher levels	879:891	higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs	879:1053	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	1	101	theme	cultivars	193:201	arg1	rice					157:160	Brown rice	151:160	Brown rice of different long-grain Indica cultivars	151:201	Brown rice of different long-grain Indica cultivars was polished to variable degree of milling (DoM) to see the difference in proteins and starches characteristics in head (HR) and broken rice (BR).
31732053	6	102	theme	51 kDa	912:917	arg1	levels					886:891	higher levels	879:891	higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs	879:1053	Milled rice from different cultivars milled to 6% DoM showed higher levels of 59 kDa, 54 kDa, 51 kDa, 32 kDa, 31 kDa, 30 kDa, 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 and 12 kDa PPs, while 28 kDa, 24 kDa, 23 kDa, 15 kDa, 13 kDa and 12 kDa PPs was the least or not observed in BR.
31732053	4	103	theme	peak	643:646	arg1	viscosity					648:656	peak viscosity	643:656	peak viscosity	643:656	The extended DoM caused a decrease in protein and fat content in both HR and BR, whereas; an increase in peak viscosity and final viscosity was observed.
31732053	2	104	theme	differential	365:376	arg1	accumulation					378:389	differential accumulation	365:389	differential accumulation of starch, fat and proteins in both HR and BR	365:435	Study revealed differential accumulation of starch, fat and proteins in both HR and BR.
30256608	4	0	theme	drugs	486:490	arg1	forms					470:474	various forms	462:474	various forms of active drugs, including fibroblast growth factor	462:526	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	7	1	theme	granulation-tissue	1149:1166	arg1	formation					1168:1176	granulation-tissue formation	1149:1176	granulation-tissue formation	1149:1176	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	8	2	theme	particles	1346:1354	arg1	value					1300:1304	the potential value	1286:1304	the potential value of multifunctional biomass inverse opal particles in biomedicine	1286:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	7	3	theme	biomass-composited	1056:1073	arg1	able					1094:1097	able	1094:1097	able	1094:1097	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	7	3	theme	biomass-composited	1056:1073	arg1	contributed					1232:1242	contributed	1232:1242	contributed to wound healing	1232:1259	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	7	3	theme	biomass-composited	1056:1073	arg1	microcarriers					1075:1087	the biomass-composited microcarriers	1052:1087	the biomass-composited microcarriers	1052:1087	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	4	4	theme	fibroblast	503:512	arg1	factor					521:526	fibroblast growth factor	503:526	fibroblast growth factor	503:526	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	8	5	from	biomedicine	1359:1369	arg1	value					1300:1304	the potential value	1286:1304	the potential value of multifunctional biomass inverse opal particles in biomedicine	1286:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	1	6	theme	medical	140:146	arg1	community					148:156	the medical community	136:156	the medical community	136:156	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	6	7	theme	real	1016:1019	arg1	time					1021:1024	real time	1016:1024	real time	1016:1024	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	7	8	theme	wound	1247:1251	arg1	healing					1253:1259	wound healing	1247:1259	wound healing	1247:1259	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	2	9	theme	drug	266:269	arg1	delivery					271:278	drug delivery	266:278	drug delivery	266:278	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	4	10	theme	inverse	572:578	arg1	particles					585:593	the inverse opal particles	568:593	the inverse opal particles	568:593	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	3	11	theme	chitosan	335:342	arg1	biomass					344:350	chitosan biomass	335:350	chitosan biomass	335:350	The particles were derived by using chitosan biomass to negatively replicate spherical colloid crystal templates.
30256608	6	12	theme	particles	923:931	arg1	peaks					886:890	characteristic reflection peaks	860:890	characteristic reflection peaks	860:890	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	6	12	theme	particles	923:931	arg1	colors					849:854	structural colors	838:854	structural colors	838:854	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	5	13	theme	composited	665:674	arg1	particles					676:684	the composited particles	661:684	the composited particles	661:684	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	8	14	from	particles	1346:1354	arg1	biomedicine					1359:1369	biomedicine	1359:1369	biomedicine	1359:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	1	15	theme	Wound	67:71	arg1	healing					73:79	Wound healing	67:79	Wound healing	67:79	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	4	16	theme	various	462:468	arg1	forms					470:474	various forms	462:474	various forms of active drugs, including fibroblast growth factor	462:526	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	7	17	theme	collagen	1124:1131	arg1	deposition					1133:1142	collagen deposition	1124:1142	collagen deposition	1124:1142	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	2	18	theme	features	253:260	arg1	series					234:239	a series	232:239	a series of advanced features for drug delivery and wound healing	232:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	0	19	theme	Wound	52:56	arg1	Healing					58:64	Wound Healing	52:64	Wound Healing	52:64	Multifunctional Chitosan Inverse Opal Particles for Wound Healing.
30256608	4	20	theme	void	553:556	arg1	spaces					558:563	the void spaces	549:563	the void spaces of the inverse opal particles	549:593	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	5	21	theme	inflammation	788:799	arg1	reaction					801:808	the inflammation reaction	784:808	the inflammation reaction at wound sites	784:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	2	22	theme	advanced	244:251	arg1	features					253:260	advanced features	244:260	advanced features for drug delivery and wound healing	244:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	8	23	theme	biomass	1325:1331	arg1	particles					1346:1354	multifunctional biomass inverse opal particles	1309:1354	multifunctional biomass inverse opal particles in biomedicine	1309:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	2	24	attach	present	177:183	arg2	we					174:175	we	174:175	we	174:175	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	2	24	attach	present	177:183	arg1	paper					167:171	this paper	162:171	this paper	162:171	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	6	25	theme	composited	899:908	arg1	particles					923:931	the composited inverse opal particles	895:931	the composited inverse opal particles	895:931	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	6	26	theme	opal	918:921	arg1	particles					923:931	the composited inverse opal particles	895:931	the composited inverse opal particles	895:931	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	0	27	theme	Multifunctional	0:14	arg1	Chitosan					16:23	Multifunctional Chitosan	0:23	Multifunctional Chitosan	0:23	Multifunctional Chitosan Inverse Opal Particles for Wound Healing.
30256608	6	28	dep	colors	849:854	arg1	the					834:836	the	834:836	the	834:836	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	6	29	theme	release	961:967	arg1	process					969:975	the release process	957:975	the release process	957:975	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	2	30	theme	wound	284:288	arg1	healing					290:296	wound healing	284:296	wound healing	284:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	8	31	theme	potential	1290:1298	arg1	value					1300:1304	the potential value	1286:1304	the potential value of multifunctional biomass inverse opal particles in biomedicine	1286:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	0	32	theme	Opal	33:36	arg1	Particles					38:46	Inverse Opal Particles	25:46	Inverse Opal Particles	25:46	Multifunctional Chitosan Inverse Opal Particles for Wound Healing.
30256608	4	33	theme	temperature-responsive	615:636	arg1	hydrogel					638:645	temperature-responsive hydrogel	615:645	temperature-responsive hydrogel	615:645	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	0	34	theme	Inverse	25:31	arg1	Particles					38:46	Inverse Opal Particles	25:46	Inverse Opal Particles	25:46	Multifunctional Chitosan Inverse Opal Particles for Wound Healing.
30256608	2	35	with	particles	217:225	arg1	series					234:239	a series	232:239	a series of advanced features for drug delivery and wound healing	232:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	5	36	theme	intelligent	709:719	arg1	release					726:732	intelligent drug release	709:732	intelligent drug release	709:732	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	4	37	theme	porous	443:448	arg1	structures					450:459	the interconnected porous structures	424:459	the interconnected porous structures	424:459	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	5	38	with	endowed	653:659	arg1	capability					695:704	the capability	691:704	the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites	691:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	3	39	theme	crystal	394:400	arg1	templates					402:410	spherical colloid crystal templates	376:410	spherical colloid crystal templates	376:410	The particles were derived by using chitosan biomass to negatively replicate spherical colloid crystal templates.
30256608	6	40	theme	drug	982:985	arg1	delivery					987:994	the drug delivery	978:994	the drug delivery	978:994	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	5	41	theme	drug	721:724	arg1	release					726:732	intelligent drug release	709:732	intelligent drug release	709:732	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	4	42	theme	interconnected	428:441	arg1	structures					450:459	the interconnected porous structures	424:459	the interconnected porous structures	424:459	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	8	43	theme	inverse	1333:1339	arg1	particles					1346:1354	multifunctional biomass inverse opal particles	1309:1354	multifunctional biomass inverse opal particles in biomedicine	1309:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	5	44	theme	release	726:732	arg1	capability					695:704	the capability	691:704	the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites	691:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	2	45	theme	opal	212:215	arg1	particles					217:225	biomass-composited inverse opal particles	185:225	biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing	185:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	8	46	from	value	1300:1304	arg1	biomedicine					1359:1369	biomedicine	1359:1369	biomedicine	1359:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	5	47	theme	high	757:760	arg1	temperature					762:772	the relatively high temperature	742:772	the relatively high temperature caused by the inflammation reaction at wound sites	742:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	3	48	theme	spherical	376:384	arg1	templates					402:410	spherical colloid crystal templates	376:410	spherical colloid crystal templates	376:410	The particles were derived by using chitosan biomass to negatively replicate spherical colloid crystal templates.
30256608	4	49	theme	growth	514:519	arg1	factor					521:526	fibroblast growth factor	503:526	fibroblast growth factor	503:526	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	5	50	theme	wound	813:817	arg1	sites					819:823	wound sites	813:823	wound sites	813:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	6	51	theme	reflection	875:884	arg1	peaks					886:890	characteristic reflection peaks	860:890	characteristic reflection peaks	860:890	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	2	52	theme	inverse	204:210	arg1	particles					217:225	biomass-composited inverse opal particles	185:225	biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing	185:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	4	53	theme	active	479:484	arg1	factor					521:526	fibroblast growth factor	503:526	fibroblast growth factor	503:526	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	4	53	theme	active	479:484	arg1	drugs					486:490	active drugs	479:490	active drugs	479:490	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	1	54	theme	important	100:108	arg1	issues					120:125	the most important and basic issues	91:125	the most important and basic issues faced by the medical community	91:156	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	3	55	theme	colloid	386:392	arg1	templates					402:410	spherical colloid crystal templates	376:410	spherical colloid crystal templates	376:410	The particles were derived by using chitosan biomass to negatively replicate spherical colloid crystal templates.
30256608	6	56	theme	characteristic	860:873	arg1	peaks					886:890	characteristic reflection peaks	860:890	characteristic reflection peaks	860:890	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	2	57	theme	biomass-composited	185:202	arg1	particles					217:225	biomass-composited inverse opal particles	185:225	biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing	185:296	In this paper, we present biomass-composited inverse opal particles with a series of advanced features for drug delivery and wound healing.
30256608	8	58	theme	opal	1341:1344	arg1	particles					1346:1354	multifunctional biomass inverse opal particles	1309:1354	multifunctional biomass inverse opal particles in biomedicine	1309:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	5	59	from	sites	819:823	arg1	reaction					801:808	the inflammation reaction	784:808	the inflammation reaction at wound sites	784:823	This endowed the composited particles with the capability of intelligent drug release through the relatively high temperature caused by the inflammation reaction at wound sites.
30256608	1	60	theme	basic	114:118	arg1	issues					120:125	the most important and basic issues	91:125	the most important and basic issues faced by the medical community	91:156	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	4	61	theme	particles	585:593	arg1	spaces					558:563	the void spaces	549:563	the void spaces of the inverse opal particles	549:593	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	1	62	theme	issues	120:125	arg1	issues					120:125	the most important and basic issues	91:125	the most important and basic issues faced by the medical community	91:156	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	1	62	theme	issues	120:125	arg1	one					84:86	one	84:86	one	84:86	Wound healing is one of the most important and basic issues faced by the medical community.
30256608	4	63	theme	opal	580:583	arg1	particles					585:593	the inverse opal particles	568:593	the inverse opal particles	568:593	Because of the interconnected porous structures, various forms of active drugs, including fibroblast growth factor could be loaded into the void spaces of the inverse opal particles and encapsulated by temperature-responsive hydrogel.
30256608	6	64	theme	structural	838:847	arg1	colors					849:854	structural colors	838:854	structural colors	838:854	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	6	65	theme	inverse	910:916	arg1	particles					923:931	the composited inverse opal particles	895:931	the composited inverse opal particles	895:931	Because the structural colors and characteristic reflection peaks of the composited inverse opal particles are blue-shifted during the release process, the drug delivery can be monitored in real time.
30256608	8	66	theme	multifunctional	1309:1323	arg1	particles					1346:1354	multifunctional biomass inverse opal particles	1309:1354	multifunctional biomass inverse opal particles in biomedicine	1309:1369	These features point to the potential value of multifunctional biomass inverse opal particles in biomedicine.
30256608	7	67	dep	able	1094:1097	arg1	able					1094:1097	able	1094:1097	able	1094:1097	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	7	67	dep	able	1094:1097	arg1	contributed					1232:1242	contributed	1232:1242	contributed to wound healing	1232:1259	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
30256608	7	67	dep	able	1094:1097	arg1	microcarriers					1075:1087	the biomass-composited microcarriers	1052:1087	the biomass-composited microcarriers	1052:1087	It was demonstrated that the biomass-composited microcarriers were able to promote angiogenesis, collagen deposition, and granulation-tissue formation as well as reduce inflammation and thus significantly contributed to wound healing.
31557651	5	0	theme	electrostatic	616:628	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	0	theme	electrostatic	616:628	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	2	1	dep	similarities	260:271	arg1	the					256:258	the	256:258	the	256:258	To investigate the similarities and differences in adsorption behaviors between Biochar and BCFe/S, characterization, kinetics, isotherms and thermodynamics were discussed.
31557651	8	2	theme	work	1070:1073	arg1	Findings					1053:1060	Findings	1053:1060	Findings of this work	1053:1073	Findings of this work could offer valuable information in designing adsorbents and investigating adsorption mechanisms.
31557651	7	3	theme	higher	1033:1038	arg1	temperature					1040:1050	higher temperature	1033:1050	higher temperature	1033:1050	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	0	4	theme	energy	90:95	arg1	distribution					97:108	site energy distribution	85:108	site energy distribution	85:108	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	6	5	theme	above	779:783	arg1	mechanisms					785:794	above mechanisms	779:794	above mechanisms	779:794	Besides above mechanisms, chelating and ion exchange were also accounted for adsorption mechanisms for TC uptake by BCFe/S-4.
31557651	0	6	theme	site	85:88	arg1	distribution					97:108	site energy distribution	85:108	site energy distribution	85:108	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	5	7	theme	π-π	642:644	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	7	theme	π-π	642:644	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	8	8	theme	valuable	1087:1094	arg1	information					1096:1106	valuable information	1087:1106	valuable information	1087:1106	Findings of this work could offer valuable information in designing adsorbents and investigating adsorption mechanisms.
31557651	8	9	theme	adsorption	1150:1159	arg1	mechanisms					1161:1170	adsorption mechanisms	1150:1170	adsorption mechanisms	1150:1170	Findings of this work could offer valuable information in designing adsorbents and investigating adsorption mechanisms.
31557651	6	10	theme	adsorption	848:857	arg1	mechanisms					859:868	adsorption mechanisms	848:868	adsorption mechanisms	848:868	Besides above mechanisms, chelating and ion exchange were also accounted for adsorption mechanisms for TC uptake by BCFe/S-4.
31557651	3	11	theme	site	429:432	arg1	distribution					441:452	site energy distribution	429:452	site energy distribution (SED)	429:458	The studies on site energy distribution (SED) were also presented.
31557651	3	11	theme	site	429:432	arg1	SED					455:457	SED	455:457	SED	455:457	The studies on site energy distribution (SED) were also presented.
31557651	0	12	theme	novel	2:6	arg1	Biochar					8:14	A novel Biochar	0:14	A novel Biochar	0:14	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	7	13	theme	SED	907:909	arg1	results					911:917	SED results	907:917	SED results	907:917	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	2	14	theme	adsorption	292:301	arg1	behaviors					303:311	adsorption behaviors	292:311	adsorption behaviors	292:311	To investigate the similarities and differences in adsorption behaviors between Biochar and BCFe/S, characterization, kinetics, isotherms and thermodynamics were discussed.
31557651	7	15	from	temperature	1040:1050	arg1	heterogeneous					1016:1028	heterogeneous	1016:1028	heterogeneous	1016:1028	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	7	15	from	temperature	1040:1050	arg1	surface					987:993	the surface	983:993	the surface of BCFe/S-4	983:1005	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	4	16	theme	adsorption	520:529	arg1	amount					531:536	the maximum TC adsorption amount	505:536	the maximum TC adsorption amount	505:536	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	4	16	theme	adsorption	520:529	arg1	51.78 mg/g					542:551	51.78 mg/g	542:551	51.78 mg/g for Biochar	542:563	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	1	17	from	water	234:238	arg1	removal					221:227	tetracycline (TC) removal	203:227	tetracycline (TC) removal from water	203:238	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S) was prepared for tetracycline (TC) removal from water.
31557651	5	18	theme	silicate	670:677	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	18	theme	silicate	670:677	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	19	theme	TC	748:749	arg1	removal					751:757	TC removal	748:757	TC removal by Biochar	748:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	0	20	from	studies	74:80	arg1	distribution					97:108	site energy distribution	85:108	site energy distribution	85:108	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	3	21	theme	energy	434:439	arg1	distribution					441:452	site energy distribution	429:452	site energy distribution (SED)	429:458	The studies on site energy distribution (SED) were also presented.
31557651	3	21	theme	energy	434:439	arg1	SED					455:457	SED	455:457	SED	455:457	The studies on site energy distribution (SED) were also presented.
31557651	0	22	theme	tetracycline	46:57	arg1	adsorption					59:68	tetracycline adsorption	46:68	tetracycline adsorption	46:68	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	5	23	theme	hydrogen	691:698	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	23	theme	hydrogen	691:698	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	7	24	theme	BCFe/S-4	998:1005	arg1	heterogeneous					1016:1028	heterogeneous	1016:1028	heterogeneous	1016:1028	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	7	24	theme	BCFe/S-4	998:1005	arg1	surface					987:993	the surface	983:993	the surface of BCFe/S-4	983:1005	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	0	25	from	adsorption	59:68	arg1	distribution					97:108	site energy distribution	85:108	site energy distribution	85:108	A novel Biochar modified by Chitosan-Fe/S for tetracycline adsorption and studies on site energy distribution.
31557651	4	26	theme	TC	517:518	arg1	amount					531:536	the maximum TC adsorption amount	505:536	the maximum TC adsorption amount	505:536	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	4	26	theme	TC	517:518	arg1	51.78 mg/g					542:551	51.78 mg/g	542:551	51.78 mg/g for Biochar	542:563	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	6	27	theme	ion	811:813	arg1	exchange					815:822	ion exchange	811:822	ion exchange	811:822	Besides above mechanisms, chelating and ion exchange were also accounted for adsorption mechanisms for TC uptake by BCFe/S-4.
31557651	1	28	theme	novel	113:117	arg1	Biochar					139:145	A novel wasted sludge-based Biochar	111:145	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S)	111:184	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S) was prepared for tetracycline (TC) removal from water.
31557651	4	29	theme	maximum	509:515	arg1	amount					531:536	the maximum TC adsorption amount	505:536	the maximum TC adsorption amount	505:536	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	4	29	theme	maximum	509:515	arg1	51.78 mg/g					542:551	51.78 mg/g	542:551	51.78 mg/g for Biochar	542:563	The results showed that the maximum TC adsorption amount was 51.78 mg/g for Biochar, while it was 183.01 mg/g for BCFe/S-4.
31557651	5	30	theme	pore	656:659	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	30	theme	pore	656:659	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	1	31	theme	wasted	119:124	arg1	Biochar					139:145	A novel wasted sludge-based Biochar	111:145	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S)	111:184	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S) was prepared for tetracycline (TC) removal from water.
31557651	1	32	theme	tetracycline	203:214	arg1	removal					221:227	tetracycline (TC) removal	203:227	tetracycline (TC) removal from water	203:238	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S) was prepared for tetracycline (TC) removal from water.
31557651	7	33	theme	Biochar	948:954	arg1	homogeneous					965:975	homogeneous	965:975	homogeneous	965:975	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	7	33	theme	Biochar	948:954	arg1	surface					937:943	the surface	933:943	the surface of Biochar	933:954	Moreover, SED results revealed that the surface of Biochar was more homogeneous while the surface of BCFe/S-4 was more heterogeneous at higher temperature.
31557651	1	34	theme	sludge-based	126:137	arg1	Biochar					139:145	A novel wasted sludge-based Biochar	111:145	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S)	111:184	A novel wasted sludge-based Biochar modified by Chitosan and Fe/S (BCFe/S) was prepared for tetracycline (TC) removal from water.
31557651	2	35	from	similarities	260:271	arg1	behaviors					303:311	adsorption behaviors	292:311	adsorption behaviors	292:311	To investigate the similarities and differences in adsorption behaviors between Biochar and BCFe/S, characterization, kinetics, isotherms and thermodynamics were discussed.
31557651	5	36	theme	main	717:720	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	36	theme	main	717:720	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	3	37	from	studies	418:424	arg1	distribution					441:452	site energy distribution	429:452	site energy distribution (SED)	429:458	The studies on site energy distribution (SED) were also presented.
31557651	3	37	from	studies	418:424	arg1	SED					455:457	SED	455:457	SED	455:457	The studies on site energy distribution (SED) were also presented.
31557651	5	38	theme	adsorption	722:731	arg1	bonding					679:685	silicate bonding	670:685	silicate bonding	670:685	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	Meanwhile					605:613	Meanwhile	605:613	Meanwhile	605:613	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	stacking					646:653	π-π stacking	642:653	π-π stacking	642:653	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	mechanisms					733:742	the main adsorption mechanisms	713:742	the main adsorption mechanisms for TC removal by Biochar	713:768	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	filling					661:667	pore filling	656:667	pore filling	656:667	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	bonding					700:706	hydrogen bonding	691:706	hydrogen bonding	691:706	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	5	38	theme	adsorption	722:731	arg1	attraction					630:639	electrostatic attraction	616:639	electrostatic attraction	616:639	Meanwhile, electrostatic attraction, π-π stacking, pore filling, silicate bonding and hydrogen bonding were the main adsorption mechanisms for TC removal by Biochar.
31557651	2	39	from	differences	277:287	arg1	behaviors					303:311	adsorption behaviors	292:311	adsorption behaviors	292:311	To investigate the similarities and differences in adsorption behaviors between Biochar and BCFe/S, characterization, kinetics, isotherms and thermodynamics were discussed.
31306677	8	0	with	interaction	1268:1278	arg1	cells					1296:1300	the immune cells	1285:1300	the immune cells	1285:1300	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	8	0	with	interaction	1268:1278	arg1	profile					1322:1328	biodistribution profile	1306:1328	biodistribution profile	1306:1328	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	2	1	theme	inflammatory	374:385	arg1	diseases					387:394	chronic inflammatory diseases	366:394	chronic inflammatory diseases	366:394	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	8	2	theme	immune	1289:1294	arg1	cells					1296:1300	the immune cells	1285:1300	the immune cells	1285:1300	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	5	3	dep	in	781:782	arg1	vitro					784:788	vitro	784:788	vitro	784:788	The in vitro results showed that small NCs interacted more efficiently with macrophages than their larger counterparts.
31306677	2	4	theme	chronic	366:372	arg1	diseases					387:394	chronic inflammatory diseases	366:394	chronic inflammatory diseases	366:394	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	2	5	from	interest	342:349	arg1	case					358:361	the case	354:361	the case of chronic inflammatory diseases	354:394	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	2	5	from	interest	342:349	arg1	cancer					397:402	cancer	397:402	cancer	397:402	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	0	6	with	interaction	65:75	arg1	macrophages					82:92	macrophages	82:92	macrophages	82:92	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	2	7	dep	response	317:324	arg1	interest					342:349	especial interest	333:349	especial interest	333:349	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	3	8	theme	composition	490:500	arg1	role					467:470	the role	463:470	the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages	463:570	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	4	9	theme	NCs	682:684	arg1	sizes					653:657	two different sizes	639:657	two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm)	639:774	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	8	10	from	changes	1201:1207	arg1	range					1227:1231	the nanometric range	1212:1231	the nanometric range	1212:1231	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	8	11	theme	different	1258:1266	arg1	interaction					1268:1278	a remarkably different interaction	1245:1278	a remarkably different interaction with the immune cells and biodistribution profile	1245:1328	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	7	12	dep	in	976:977	arg1	vivo					979:982	vivo	979:982	vivo	979:982	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	1	13	theme	immune	172:177	arg1	system					179:184	the innate immune system	161:184	the innate immune system specialized in the phagocytosis of foreign elements	161:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	8	14	theme	small	1195:1199	arg1	changes					1201:1207	small changes	1195:1207	small changes in the nanometric range	1195:1231	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	1	15	theme	system	179:184	arg1	Macrophages					128:138	Macrophages	128:138	Macrophages	128:138	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	1	15	theme	system	179:184	arg1	cells					152:156	pivotal cells	144:156	pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements	144:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	7	16	theme	size	1148:1151	arg1	counterparts					1153:1164	their medium size counterparts	1135:1164	their medium size counterparts	1135:1164	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	4	17	theme	inulin	662:667	arg1	NCs					682:684	inulin and chitosan NCs	662:684	NCs	682:684	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	17	theme	inulin	662:667	arg1	small					736:740	small	736:740	small	736:740	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	17	theme	inulin	662:667	arg1	medium					756:761	medium	756:761	medium (170-250 nm)	756:774	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	6	18	theme	chitosan	943:950	arg1	NCs					952:954	chitosan NCs	943:954	chitosan NCs	943:954	Inulin NCs were significantly less toxic than chitosan NCs.
31306677	8	19	theme	nanometric	1216:1225	arg1	range					1227:1231	the nanometric range	1212:1231	the nanometric range	1212:1231	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	0	20	with	biodistribution	98:112	arg1	macrophages					82:92	macrophages	82:92	macrophages	82:92	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	2	21	theme	especial	333:340	arg1	interest					342:349	especial interest	333:349	especial interest	333:349	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	7	22	theme	small	1041:1045	arg1	NCs					1047:1049	small NCs	1041:1049	small NCs	1041:1049	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	3	23	theme	size	475:478	arg1	role					467:470	the role	463:470	the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages	463:570	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	5	24	theme	in	781:782	arg1	results					790:796	The in vitro results	777:796	The in vitro results	777:796	The in vitro results showed that small NCs interacted more efficiently with macrophages than their larger counterparts.
31306677	1	25	theme	innate	165:170	arg1	system					179:184	the innate immune system	161:184	the innate immune system specialized in the phagocytosis of foreign elements	161:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	0	26	theme	polymeric	28:36	arg1	nanocapsules					38:49	polymeric nanocapsules	28:49	polymeric nanocapsules	28:49	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	5	27	theme	larger	876:881	arg1	counterparts					883:894	their larger counterparts	870:894	their larger counterparts	870:894	The in vitro results showed that small NCs interacted more efficiently with macrophages than their larger counterparts.
31306677	2	28	theme	vaccine	412:418	arg1	development					420:430	vaccine development	412:430	vaccine development	412:430	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	0	29	from	interaction	65:75	arg1	zebrafish					117:125	zebrafish	117:125	zebrafish	117:125	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	3	30	from	role	467:470	arg1	interaction					543:553	their interaction	537:553	their interaction with macrophages	537:570	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	1	31	theme	foreign	221:227	arg1	elements					229:236	foreign elements	221:236	foreign elements	221:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	3	32	with	interaction	543:553	arg1	macrophages					560:570	macrophages	560:570	macrophages	560:570	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	3	33	theme	nanocapsules	515:526	arg1	size					475:478	size	475:478	size	475:478	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	3	33	theme	nanocapsules	515:526	arg1	composition					490:500	shell composition	484:500	shell composition	484:500	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	0	34	theme	nanocapsules	38:49	arg1	composition					13:23	composition	13:23	composition	13:23	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	0	34	theme	nanocapsules	38:49	arg1	size					4:7	size	4:7	size	4:7	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	3	35	theme	polymeric	505:513	arg1	NCs					529:531	NCs	529:531	NCs	529:531	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	3	35	theme	polymeric	505:513	arg1	nanocapsules					515:526	polymeric nanocapsules	505:526	polymeric nanocapsules (NCs)	505:532	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	6	36	theme	Inulin	897:902	arg1	NCs					904:906	Inulin NCs	897:906	Inulin NCs	897:906	Inulin NCs were significantly less toxic than chitosan NCs.
31306677	7	37	theme	in	976:977	arg1	intravenous/intramuscular					1000:1024	intravenous/intramuscular	1000:1024	intravenous/intramuscular	1000:1024	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	7	37	theme	in	976:977	arg1	administration					984:997	in vivo administration	976:997	in vivo administration (intravenous/intramuscular) to zebrafish	976:1038	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	3	38	theme	shell	484:488	arg1	composition					490:500	shell composition	484:500	shell composition	484:500	This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo.
31306677	1	39	theme	elements	229:236	arg1	phagocytosis					205:216	the phagocytosis	201:216	the phagocytosis of foreign elements	201:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	0	40	from	biodistribution	98:112	arg1	zebrafish					117:125	zebrafish	117:125	zebrafish	117:125	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	5	41	theme	small	810:814	arg1	NCs					816:818	small NCs	810:818	small NCs	810:818	The in vitro results showed that small NCs interacted more efficiently with macrophages than their larger counterparts.
31306677	1	42	theme	pivotal	144:150	arg1	Macrophages					128:138	Macrophages	128:138	Macrophages	128:138	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	1	42	theme	pivotal	144:150	arg1	cells					152:156	pivotal cells	144:156	pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements	144:236	Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements.
31306677	8	43	theme	biodistribution	1306:1320	arg1	profile					1322:1328	biodistribution profile	1306:1328	biodistribution profile	1306:1328	These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
31306677	4	44	theme	charged	713:719	arg1	NCs					682:684	inulin and chitosan NCs	662:684	NCs	682:684	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	44	theme	charged	713:719	arg1	small					736:740	small	736:740	small	736:740	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	44	theme	charged	713:719	arg1	medium					756:761	medium	756:761	medium (170-250 nm)	756:774	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	0	45	dep	size	4:7	arg1	The					0:2	The	0:2	The	0:2	The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish.
31306677	4	46	theme	chitosan	673:680	arg1	NCs					682:684	inulin and chitosan NCs	662:684	NCs	682:684	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	46	theme	chitosan	673:680	arg1	small					736:740	small	736:740	small	736:740	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	46	theme	chitosan	673:680	arg1	medium					756:761	medium	756:761	medium (170-250 nm)	756:774	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	4	47	theme	different	643:651	arg1	sizes					653:657	two different sizes	639:657	two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm)	639:774	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31306677	7	48	theme	medium	1141:1146	arg1	counterparts					1153:1164	their medium size counterparts	1135:1164	their medium size counterparts	1135:1164	Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts.
31306677	2	49	theme	diseases	387:394	arg1	case					358:361	the case	354:361	the case of chronic inflammatory diseases	354:394	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	2	49	theme	diseases	387:394	arg1	cancer					397:402	cancer	397:402	cancer	397:402	Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development.
31306677	4	50	theme	systematic	602:611	arg1	study					613:617	A systematic study	600:617	A systematic study	600:617	A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm).
31466644	6	0	theme	polyhedral	946:955	arg1	shape					957:961	polyhedral shape	946:961	polyhedral shape	946:961	The starches presented polyhedral shape and small size with an average diameter of 5.4 μm.
31466644	14	1	theme	physicochemical	1961:1975	arg1	similar					2125:2131	similar	2125:2131	similar	2125:2131	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	14	1	theme	physicochemical	1961:1975	arg1	characteristics					1989:2003	The physicochemical and thermal characteristics	1957:2003	The physicochemical and thermal characteristics of the obtained starches	1957:2028	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	3	2	theme	starches	588:595	arg1	characteristics					547:561	Morphological and physicochemical characteristics	513:561	Morphological and physicochemical characteristics of the young bamboo culm starches	513:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	11	3	theme	short	1601:1605	arg1	chains					1607:1612	short chains	1601:1612	short chains	1601:1612	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	12	4	theme	high	1734:1737	arg1	>78 °C					1767:1772	>78 °C	1767:1772	>78 °C	1767:1772	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	12	4	theme	high	1734:1737	arg1	temperature					1754:1764	high gelatinization temperature	1734:1764	high gelatinization temperature (>78 °C)	1734:1773	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	4	5	theme	high	771:774	arg1	luminosity					776:785	high luminosity	771:785	high luminosity (L* > 89)	771:795	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	4	5	theme	high	771:774	arg1	*					789:789	L* > 89	788:794	L* > 89	788:794	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	13	6	theme	starches	1915:1922	arg1	lower					1929:1933	lower	1929:1933	lower	1929:1933	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	13	6	theme	starches	1915:1922	arg1	temperatures					1887:1898	The gelatinization temperatures	1868:1898	The gelatinization temperatures of gelatinized starches	1868:1922	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	1	7	with	composition	284:294	arg1	starch					309:314	starch	309:314	starch	309:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	1	7	with	composition	284:294	arg1	%					304:304	>10%	301:304	>10% of starch	301:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	14	8	theme	non-food	2185:2192	arg1	products					2194:2201	non-food products	2185:2201	non-food products	2185:2201	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	4	9	theme	yellow	747:752	arg1	coloration					754:763	pale yellow coloration	742:763	pale yellow coloration	742:763	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	6	10	theme	5.4 μm	1006:1011	arg1	diameter					994:1001	an average diameter	983:1001	an average diameter of 5.4 μm	983:1011	The starches presented polyhedral shape and small size with an average diameter of 5.4 μm.
31466644	3	11	theme	obtained	649:656	arg1	data					658:661	the obtained data	645:661	the obtained data	645:661	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	14	12	theme	native	2108:2113	arg1	granule					2100:2106	the starch granule	2089:2106	the starch granule native	2089:2113	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	1	13	theme	new	86:88	arg1	ingredients					102:112	new and natural ingredients	86:112	new and natural ingredients	86:112	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	9	14	theme	size	1275:1278	arg1	distribution					1280:1291	molecular size distribution	1265:1291	molecular size distribution of starch chains	1265:1308	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	2	15	theme	culm	465:468	arg1	parts					414:418	three different parts	398:418	three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively)	398:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	15	theme	culm	465:468	arg1	top					440:442	top	440:442	top	440:442	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	15	theme	culm	465:468	arg1	middle					429:434	middle	429:434	middle	429:434	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	15	theme	culm	465:468	arg1	bottom					421:426	bottom	421:426	bottom	421:426	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	12	16	theme	molecular	1844:1852	arg1	organization					1854:1865	the greater molecular organization	1832:1865	the greater molecular organization	1832:1865	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	1	17	theme	bamboo	191:196	arg1	culm					198:201	the fresh young bamboo culm	175:201	the fresh young bamboo culm of Dendrocalamus asper	175:224	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	0	18	from	asper	64:68	arg1	starch					38:43	young bamboo culm starch	20:43	young bamboo culm starch from Dendrocalamus asper	20:68	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	0	18	from	asper	64:68	arg1	Characterization					0:15	Characterization	0:15	Characterization of young bamboo culm starch from Dendrocalamus asper.	0:69	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	9	19	theme	chains	1303:1308	arg1	distribution					1280:1291	molecular size distribution	1265:1291	molecular size distribution of starch chains	1265:1308	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	3	20	theme	Morphological	513:525	arg1	characteristics					547:561	Morphological and physicochemical characteristics	513:561	Morphological and physicochemical characteristics of the young bamboo culm starches	513:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	12	21	theme	enthalpies	1783:1792	arg1	values					1794:1799	low enthalpies values	1779:1799	low enthalpies values (<6.35 J·g-1)	1779:1813	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	12	21	theme	enthalpies	1783:1792	arg1	<6.35 J·g-1					1802:1812	<6.35 J·g-1	1802:1812	<6.35 J·g-1	1802:1812	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	13	22	theme	gelatinized	1903:1913	arg1	starches					1915:1922	gelatinized starches	1903:1922	gelatinized starches	1903:1922	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	2	23	theme	asper	476:480	arg1	culm					465:468	the young bamboo culm	448:468	the young bamboo culm of D. asper (SB, SM and ST, respectively)	448:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	14	24	theme	extraction	2066:2075	arg1	success					2051:2057	the success	2047:2057	the success of the extraction, which keep the starch granule native,	2047:2114	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	1	25	theme	starch	309:314	arg1	starch					309:314	starch	309:314	starch	309:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	1	25	theme	starch	309:314	arg1	%					304:304	>10%	301:304	>10% of starch	301:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	2	26	theme	young	452:456	arg1	culm					465:468	the young bamboo culm	448:468	the young bamboo culm of D. asper (SB, SM and ST, respectively)	448:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	0	27	from	Characterization	0:15	arg1	asper					64:68	asper	64:68	asper	64:68	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	8	28	theme	apparent	1160:1167	arg1	content					1177:1183	apparent amylose content	1160:1183	apparent amylose content similar to starches from cereals	1160:1216	ST and SB showed apparent amylose content similar to starches from cereals and isolated from bamboo seeds.
31466644	7	29	theme	low	1040:1042	arg1	moisture					1044:1051	low moisture	1040:1051	low moisture (7.0 g/100 g)	1040:1065	All the samples presented low moisture (7.0 g/100 g), protein (2.0 g/100 g), lipid (0.3 g/100 g) and ash (1.0 g/100 g) contents.
31466644	7	29	theme	low	1040:1042	arg1	7.0 g/100 g					1054:1064	7.0 g/100 g	1054:1064	7.0 g/100 g	1054:1064	All the samples presented low moisture (7.0 g/100 g), protein (2.0 g/100 g), lipid (0.3 g/100 g) and ash (1.0 g/100 g) contents.
31466644	5	30	theme	SEM	833:835	arg1	images					837:842	SEM images	833:842	SEM images	833:842	SEM images showed compound granules, which under polarized light exhibit a Maltese cross.
31466644	1	31	theme	industrial	251:260	arg1	production					262:271	industrial production	251:271	industrial production due to its composition with >10% of starch	251:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	4	32	from	luminosity	776:785	arg1	region					825:830	the red region	817:830	the red region	817:830	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	9	33	theme	higher	1364:1369	arg1	levels					1357:1362	amylopectin levels	1345:1362	amylopectin levels higher than those of amylose	1345:1391	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	14	34	theme	obtained	2012:2019	arg1	starches					2021:2028	the obtained starches	2008:2028	the obtained starches	2008:2028	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	2	35	theme	aqueous	375:381	arg1	solution					383:390	aqueous solution	375:390	aqueous solution	375:390	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	0	36	theme	young	20:24	arg1	starch					38:43	young bamboo culm starch	20:43	young bamboo culm starch from Dendrocalamus asper	20:68	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	1	37	theme	due	273:275	arg1	production					262:271	industrial production	251:271	industrial production due to its composition with >10% of starch	251:314	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	1	38	theme	natural	94:100	arg1	ingredients					102:112	new and natural ingredients	86:112	new and natural ingredients	86:112	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	11	39	theme	A-type	1643:1648	arg1	polymorph					1650:1658	the A-type polymorph	1639:1658	the A-type polymorph presented	1639:1668	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	10	40	theme	amylopectin	1442:1452	arg1	length					1432:1437	The chain length	1422:1437	The chain length of amylopectin	1422:1452	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	0	41	theme	culm	33:36	arg1	starch					38:43	young bamboo culm starch	20:43	young bamboo culm starch from Dendrocalamus asper	20:68	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	13	42	theme	native	1944:1949	arg1	ones					1951:1954	the native ones	1940:1954	the native ones	1940:1954	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	3	43	theme	physicochemical	531:545	arg1	characteristics					547:561	Morphological and physicochemical characteristics	513:561	Morphological and physicochemical characteristics of the young bamboo culm starches	513:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	11	44	theme	higher	1580:1585	arg1	proportion					1587:1596	higher proportion	1580:1596	higher proportion	1580:1596	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	5	45	theme	polarized	882:890	arg1	light					892:896	polarized light	882:896	polarized light	882:896	SEM images showed compound granules, which under polarized light exhibit a Maltese cross.
31466644	7	46	dep	moisture	1044:1051	arg1	contents					1133:1140	contents	1133:1140	contents	1133:1140	All the samples presented low moisture (7.0 g/100 g), protein (2.0 g/100 g), lipid (0.3 g/100 g) and ash (1.0 g/100 g) contents.
31466644	3	47	theme	culm	583:586	arg1	starches					588:595	the young bamboo culm starches	566:595	the young bamboo culm starches	566:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	6	48	theme	small	967:971	arg1	size					973:976	small size	967:976	small size	967:976	The starches presented polyhedral shape and small size with an average diameter of 5.4 μm.
31466644	3	49	theme	young	570:574	arg1	starches					588:595	the young bamboo culm starches	566:595	the young bamboo culm starches	566:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	11	50	theme	chains	1607:1612	arg1	proportion					1587:1596	higher proportion	1580:1596	higher proportion	1580:1596	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	1	51	theme	Dendrocalamus	206:218	arg1	asper					220:224	Dendrocalamus asper	206:224	Dendrocalamus asper	206:224	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	2	52	dep	asper	476:480	arg1	ST					494:495	ST	494:495	ST	494:495	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	52	dep	asper	476:480	arg1	SM					487:488	SM	487:488	SM	487:488	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	52	dep	asper	476:480	arg1	SB					483:484	SB	483:484	SB	483:484	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	4	53	from	index	808:812	arg1	region					825:830	the red region	817:830	the red region	817:830	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	10	54	from	DP	1481:1482	arg1	peak					1473:1476	the main peak	1464:1476	the main peak at DP 12-13	1464:1488	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	10	55	theme	at	1508:1509	arg1	DP					1511:1512	at DP 43	1508:1515	at DP 43	1508:1515	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	8	56	from	cereals	1210:1216	arg1	starches					1196:1203	starches	1196:1203	starches from cereals	1196:1216	ST and SB showed apparent amylose content similar to starches from cereals and isolated from bamboo seeds.
31466644	1	57	theme	non-food	150:157	arg1	industries					159:168	the food and non-food industries	137:168	the food and non-food industries	137:168	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	12	58	theme	gelatinization	1739:1752	arg1	>78 °C					1767:1772	>78 °C	1767:1772	>78 °C	1767:1772	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	12	58	theme	gelatinization	1739:1752	arg1	temperature					1754:1764	high gelatinization temperature	1734:1764	high gelatinization temperature (>78 °C)	1734:1773	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	3	59	theme	thermal	621:627	arg1	properties					629:638	thermal properties	621:638	thermal properties	621:638	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	4	60	theme	pale	742:745	arg1	coloration					754:763	pale yellow coloration	742:763	pale yellow coloration	742:763	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	10	61	theme	similar	1518:1524	arg1	DP					1511:1512	at DP 43	1508:1515	at DP 43	1508:1515	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	9	62	theme	molecular	1265:1273	arg1	distribution					1280:1291	molecular size distribution	1265:1291	molecular size distribution of starch chains	1265:1308	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	6	63	theme	average	986:992	arg1	diameter					994:1001	an average diameter	983:1001	an average diameter of 5.4 μm	983:1011	The starches presented polyhedral shape and small size with an average diameter of 5.4 μm.
31466644	1	64	theme	young	185:189	arg1	culm					198:201	the fresh young bamboo culm	175:201	the fresh young bamboo culm of Dendrocalamus asper	175:224	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	2	65	theme	bamboo	458:463	arg1	culm					465:468	the young bamboo culm	448:468	the young bamboo culm of D. asper (SB, SM and ST, respectively)	448:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	12	66	theme	greater	1836:1842	arg1	organization					1854:1865	the greater molecular organization	1832:1865	the greater molecular organization	1832:1865	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	13	67	theme	gelatinization	1872:1885	arg1	lower					1929:1933	lower	1929:1933	lower	1929:1933	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	13	67	theme	gelatinization	1872:1885	arg1	temperatures					1887:1898	The gelatinization temperatures	1868:1898	The gelatinization temperatures of gelatinized starches	1868:1922	The gelatinization temperatures of gelatinized starches were lower than the native ones.
31466644	9	68	theme	starch	1296:1301	arg1	chains					1303:1308	starch chains	1296:1308	starch chains	1296:1308	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	12	69	theme	thermal	1688:1694	arg1	properties					1696:1705	about thermal properties	1682:1705	about thermal properties	1682:1705	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	14	70	theme	starch	2093:2098	arg1	granule					2100:2106	the starch granule	2089:2106	the starch granule native	2089:2113	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	4	71	theme	L	788:788	arg1	luminosity					776:785	high luminosity	771:785	high luminosity (L* > 89)	771:795	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	4	71	theme	L	788:788	arg1	*					789:789	L* > 89	788:794	L* > 89	788:794	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	5	72	theme	Maltese	908:914	arg1	cross					916:920	a Maltese cross	906:920	a Maltese cross	906:920	SEM images showed compound granules, which under polarized light exhibit a Maltese cross.
31466644	10	73	from	DP	1511:1512	arg1	at					1508:1509	at DP 43	1508:1515	at DP 43	1508:1515	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	4	74	theme	red	821:823	arg1	region					825:830	the red region	817:830	the red region	817:830	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	12	75	theme	low	1779:1781	arg1	values					1794:1799	low enthalpies values	1779:1799	low enthalpies values (<6.35 J·g-1)	1779:1813	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	12	75	theme	low	1779:1781	arg1	<6.35 J·g-1					1802:1812	<6.35 J·g-1	1802:1812	<6.35 J·g-1	1802:1812	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	3	76	theme	Scott-Knot	691:700	arg1	test					702:705	Scott-Knot test	691:705	Scott-Knot test (p < 0.05)	691:716	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	3	76	theme	Scott-Knot	691:700	arg1	p < 0.05					708:715	p < 0.05	708:715	p < 0.05	708:715	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	2	77	dep	parts	414:418	arg1	parts					414:418	three different parts	398:418	three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively)	398:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	77	dep	parts	414:418	arg1	top					440:442	top	440:442	top	440:442	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	77	dep	parts	414:418	arg1	middle					429:434	middle	429:434	middle	429:434	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	77	dep	parts	414:418	arg1	bottom					421:426	bottom	421:426	bottom	421:426	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	1	78	theme	food	141:144	arg1	industries					159:168	the food and non-food industries	137:168	the food and non-food industries	137:168	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	4	79	theme	lower	802:806	arg1	index					808:812	lower index	802:812	lower index in the red region	802:830	The starches presented pale yellow coloration, with high luminosity (L* > 89), and lower index in the red region.
31466644	1	80	theme	fresh	179:183	arg1	culm					198:201	the fresh young bamboo culm	175:201	the fresh young bamboo culm of Dendrocalamus asper	175:224	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
31466644	10	81	theme	chain	1426:1430	arg1	length					1432:1437	The chain length	1422:1437	The chain length of amylopectin	1422:1452	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	8	82	theme	amylose	1169:1175	arg1	content					1177:1183	apparent amylose content	1160:1183	apparent amylose content similar to starches from cereals	1160:1216	ST and SB showed apparent amylose content similar to starches from cereals and isolated from bamboo seeds.
31466644	14	83	theme	starches	2021:2028	arg1	similar					2125:2131	similar	2125:2131	similar	2125:2131	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	14	83	theme	starches	2021:2028	arg1	characteristics					1989:2003	The physicochemical and thermal characteristics	1957:2003	The physicochemical and thermal characteristics of the obtained starches	1957:2028	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	0	84	theme	bamboo	26:31	arg1	starch					38:43	young bamboo culm starch	20:43	young bamboo culm starch from Dendrocalamus asper	20:68	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	5	85	theme	compound	851:858	arg1	granules					860:867	compound granules	851:867	compound granules	851:867	SEM images showed compound granules, which under polarized light exhibit a Maltese cross.
31466644	8	86	theme	similar	1185:1191	arg1	content					1177:1183	apparent amylose content	1160:1183	apparent amylose content similar to starches from cereals	1160:1216	ST and SB showed apparent amylose content similar to starches from cereals and isolated from bamboo seeds.
31466644	0	87	theme	starch	38:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of young bamboo culm starch from Dendrocalamus asper.	0:69	Characterization of young bamboo culm starch from Dendrocalamus asper.
31466644	12	88	dep	presented	1724:1732	arg1	indicates					1822:1830	indicates	1822:1830	indicates the greater molecular organization	1822:1865	Concerning about thermal properties, all the samples presented high gelatinization temperature (>78 °C) and low enthalpies values (<6.35 J·g-1), which indicates the greater molecular organization.
31466644	11	89	contain	has	1576:1578	arg1	chain					1570:1574	Its chain	1566:1574	Its chain	1566:1574	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	11	89	contain	has	1576:1578	arg2	proportion					1587:1596	higher proportion	1580:1596	higher proportion	1580:1596	Its chain has higher proportion of short chains, which corroborates with the A-type polymorph presented.
31466644	2	90	theme	different	404:412	arg1	parts					414:418	three different parts	398:418	three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively)	398:510	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	90	theme	different	404:412	arg1	top					440:442	top	440:442	top	440:442	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	90	theme	different	404:412	arg1	middle					429:434	middle	429:434	middle	429:434	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	2	90	theme	different	404:412	arg1	bottom					421:426	bottom	421:426	bottom	421:426	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	3	91	theme	bamboo	576:581	arg1	starches					588:595	the young bamboo culm starches	566:595	the young bamboo culm starches	566:595	Morphological and physicochemical characteristics of the young bamboo culm starches were evaluated, besides thermal properties, and the obtained data were evaluated by ANOVA and Scott-Knot test (p < 0.05).
31466644	2	92	dep	characterize	341:352	arg1	extracted					362:370	extracted	362:370	extracted	362:370	So, this study aimed to characterize starch, extracted in aqueous solution, from three different parts (bottom, middle and top) of the young bamboo culm of D. asper (SB, SM and ST, respectively).
31466644	9	93	theme	amylopectin	1345:1355	arg1	levels					1357:1362	amylopectin levels	1345:1362	amylopectin levels higher than those of amylose	1345:1391	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	10	94	theme	main	1468:1471	arg1	peak					1473:1476	the main peak	1464:1476	the main peak at DP 12-13	1464:1488	The chain length of amylopectin presented the main peak at DP 12-13 and the second on at DP 43, similar to cereals like wheat, rice and barley.
31466644	9	95	theme	normal	1405:1410	arg1	starches					1412:1419	normal starches	1405:1419	normal starches	1405:1419	This agress to molecular size distribution of starch chains, since the SB, SM and ST presented amylopectin levels higher than those of amylose, as well as normal starches.
31466644	14	96	theme	thermal	1981:1987	arg1	similar					2125:2131	similar	2125:2131	similar	2125:2131	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	14	96	theme	thermal	1981:1987	arg1	characteristics					1989:2003	The physicochemical and thermal characteristics	1957:2003	The physicochemical and thermal characteristics of the obtained starches	1957:2028	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	8	97	theme	bamboo	1236:1241	arg1	seeds					1243:1247	bamboo seeds	1236:1247	bamboo seeds	1236:1247	ST and SB showed apparent amylose content similar to starches from cereals and isolated from bamboo seeds.
31466644	14	98	theme	other	2145:2149	arg1	starches					2151:2158	other starches	2145:2158	other starches already used in food and non-food products	2145:2201	The physicochemical and thermal characteristics of the obtained starches corroborate with the success of the extraction, which keep the starch granule native, and were similar to those of other starches already used in food and non-food products.
31466644	1	99	theme	asper	220:224	arg1	culm					198:201	the fresh young bamboo culm	175:201	the fresh young bamboo culm of Dendrocalamus asper	175:224	The search for new and natural ingredients has been stimulated by the food and non-food industries, and the fresh young bamboo culm of Dendrocalamus asper emerges as promising for industrial production due to its composition with >10% of starch.
30942566	6	0	theme	best	922:925	arg1	results					935:941	the best current results	918:941	the best current results	918:941	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	9	1	theme	ligand-protein	1242:1255	arg1	interactions					1257:1268	ligand-protein interactions	1242:1268	ligand-protein interactions	1242:1268	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	8	2	from	sensor	1133:1138	arg1	detection					1143:1151	detection	1143:1151	detection of human serum and cancer cells	1143:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	4	3	theme	carbon	420:425	arg1	composites					436:445	carbon nanotube composites	420:445	carbon nanotube composites	420:445	We assembled carbon nanotube composites on the electrode to enhance electronic conductivity by 6.2-fold and reduce overpotential with a shift of 77 mV.
30942566	8	4	theme	sensor	1133:1138	arg1	applications					1113:1124	applications	1113:1124	applications of the sensor in detection of human serum and cancer cells	1113:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	6	5	theme	ng/mL	820:824	arg1	range					802:806	a dynamic range	792:806	a dynamic range of 0.01-100 ng/mL	792:824	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	6	5	theme	ng/mL	820:824	arg1	limit					840:844	detection limit	830:844	detection limit of 5.94 pg/mL	830:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	1	6	from	Detection	74:82	arg1	biosystems					101:110	biosystems	101:110	biosystems	101:110	Detection of biomarkers in biosystems plays a key role in advanced biodiagnostics for research and clinical use.
30942566	9	7	theme	sensor	1226:1231	arg1	types					1233:1237	more sensor types	1221:1237	more sensor types	1221:1237	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	6	8	theme	comparable	904:913	arg1	amplification					889:901	amplification	889:901	amplification	889:901	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	5	9	theme	carbon	612:617	arg1	nanotubes					619:627	carbon nanotubes	612:627	carbon nanotubes	612:627	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	2	10	theme	subsequent	285:294	arg1	quantitation					296:307	subsequent quantitation	285:307	subsequent quantitation	285:307	Design of new analytical platforms is challenging and in demand, addressing molecular capture and subsequent quantitation.
30942566	7	11	with	stability	1074:1082	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	7	12	dep	=	1045:1045	arg1	RSD					1038:1040	RSD	1038:1040	RSD	1038:1040	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	7	13	theme	long-term	1064:1072	arg1	stability					1074:1082	long-term stability	1064:1082	long-term stability	1064:1082	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	4	14	theme	nanotube	427:434	arg1	composites					436:445	carbon nanotube composites	420:445	carbon nanotube composites	420:445	We assembled carbon nanotube composites on the electrode to enhance electronic conductivity by 6.2-fold and reduce overpotential with a shift of 77 mV.
30942566	7	15	dep	deviation	1027:1035	arg1	=					1045:1045	=	1045:1045	=	1045:1045	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	1	16	theme	clinical	173:180	arg1	use					182:184	clinical use	173:184	clinical use	173:184	Detection of biomarkers in biosystems plays a key role in advanced biodiagnostics for research and clinical use.
30942566	5	17	theme	electrostatic	633:645	arg1	interaction					647:657	electrostatic interaction	633:657	electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA)	633:718	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	1	18	theme	biomarkers	87:96	arg1	Detection					74:82	Detection	74:82	Detection of biomarkers in biosystems	74:110	Detection of biomarkers in biosystems plays a key role in advanced biodiagnostics for research and clinical use.
30942566	0	19	theme	Electrochemical	11:25	arg1	Sensor					27:32	Label-Free Electrochemical Sensor	0:32	Label-Free Electrochemical Sensor for CD44 by Ligand-Protein Interaction.	0:72	Label-Free Electrochemical Sensor for CD44 by Ligand-Protein Interaction.
30942566	9	20	from	interfaces	1299:1308	arg1	biosystems					1319:1328	given biosystems	1313:1328	given biosystems for diagnosis	1313:1342	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	6	21	theme	dynamic	794:800	arg1	range					802:806	a dynamic range	792:806	a dynamic range of 0.01-100 ng/mL	792:824	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	0	22	theme	Label-Free	0:9	arg1	Sensor					27:32	Label-Free Electrochemical Sensor	0:32	Label-Free Electrochemical Sensor for CD44 by Ligand-Protein Interaction.	0:72	Label-Free Electrochemical Sensor for CD44 by Ligand-Protein Interaction.
30942566	9	23	theme	interfaces	1299:1308	arg1	design					1289:1294	design	1289:1294	design of interfaces in given biosystems for diagnosis	1289:1342	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	9	24	theme	types	1233:1237	arg1	development					1206:1216	the development	1202:1216	the development of more sensor types	1202:1237	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	8	25	from	detection	1143:1151	arg1	applications					1113:1124	applications	1113:1124	applications of the sensor in detection of human serum and cancer cells	1113:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	3	26	theme	ligand-protein	379:392	arg1	interaction					394:404	ligand-protein interaction	379:404	ligand-protein interaction	379:404	Herein, we developed a label-free electrochemical sensor for CD44 by ligand-protein interaction.
30942566	5	27	theme	nanotubes	619:627	arg1	surface					601:607	the surface	597:607	the surface of carbon nanotubes	597:627	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	7	28	theme	high	970:973	arg1	selectivity					975:985	high selectivity	970:985	high selectivity	970:985	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	9	29	from	biosystems	1319:1328	arg1	design					1289:1294	design	1289:1294	design of interfaces in given biosystems for diagnosis	1289:1342	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	8	30	theme	serum	1162:1166	arg1	detection					1143:1151	detection	1143:1151	detection of human serum and cancer cells	1143:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	9	31	theme	given	1313:1317	arg1	biosystems					1319:1328	given biosystems	1313:1328	given biosystems for diagnosis	1313:1342	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	7	32	theme	%	1057:1057	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	4	33	theme	electronic	475:484	arg1	conductivity					486:497	electronic conductivity	475:497	electronic conductivity	475:497	We assembled carbon nanotube composites on the electrode to enhance electronic conductivity by 6.2-fold and reduce overpotential with a shift of 77 mV.
30942566	8	34	theme	cells	1179:1183	arg1	detection					1143:1151	detection	1143:1151	detection of human serum and cancer cells	1143:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	8	35	from	applications	1113:1124	arg1	detection					1143:1151	detection	1143:1151	detection of human serum and cancer cells	1143:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	8	36	theme	cancer	1172:1177	arg1	cells					1179:1183	cancer cells	1172:1183	cancer cells	1172:1183	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	4	37	theme	mV	555:556	arg1	shift					543:547	a shift	541:547	a shift of 77 mV	541:556	We assembled carbon nanotube composites on the electrode to enhance electronic conductivity by 6.2-fold and reduce overpotential with a shift of 77 mV.
30942566	6	38	theme	CD44	782:785	arg1	sensing					771:777	direct electrochemical sensing	748:777	direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL	748:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	5	39	theme	diallyldimethylammonium	679:701	arg1	poly					674:677	poly	674:677	poly(diallyldimethylammonium chloride) (PDDA)	674:718	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	5	39	theme	diallyldimethylammonium	679:701	arg1	chloride					703:710	diallyldimethylammonium chloride	679:710	diallyldimethylammonium chloride	679:710	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	6	40	theme	pg/mL	854:858	arg1	range					802:806	a dynamic range	792:806	a dynamic range of 0.01-100 ng/mL	792:824	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	6	40	theme	pg/mL	854:858	arg1	limit					840:844	detection limit	830:844	detection limit of 5.94 pg/mL	830:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	5	41	theme	hyaluronic	573:582	arg1	HA					590:591	HA	590:591	HA	590:591	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	5	41	theme	hyaluronic	573:582	arg1	acid					584:587	hyaluronic acid	573:587	hyaluronic acid (HA)	573:592	We conjugated hyaluronic acid (HA) to the surface of carbon nanotubes via electrostatic interaction between HA and poly(diallyldimethylammonium chloride) (PDDA).
30942566	1	42	theme	key	120:122	arg1	role					124:127	a key role	118:127	a key role	118:127	Detection of biomarkers in biosystems plays a key role in advanced biodiagnostics for research and clinical use.
30942566	0	43	theme	Ligand-Protein	46:59	arg1	Interaction					61:71	Ligand-Protein Interaction	46:71	Ligand-Protein Interaction	46:71	Label-Free Electrochemical Sensor for CD44 by Ligand-Protein Interaction.
30942566	2	44	theme	platforms	212:220	arg1	Design					187:192	Design	187:192	Design of new analytical platforms	187:220	Design of new analytical platforms is challenging and in demand, addressing molecular capture and subsequent quantitation.
30942566	9	45	theme	more	1221:1224	arg1	types					1233:1237	more sensor types	1221:1237	more sensor types	1221:1237	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30942566	7	46	theme	standard	1018:1025	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	7	47	with	selectivity	975:985	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	2	48	theme	analytical	201:210	arg1	platforms					212:220	new analytical platforms	197:220	new analytical platforms	197:220	Design of new analytical platforms is challenging and in demand, addressing molecular capture and subsequent quantitation.
30942566	2	49	theme	molecular	263:271	arg1	capture					273:279	molecular capture	263:279	molecular capture	263:279	Design of new analytical platforms is challenging and in demand, addressing molecular capture and subsequent quantitation.
30942566	6	50	theme	electrochemical	755:769	arg1	sensing					771:777	direct electrochemical sensing	748:777	direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL	748:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	6	51	with	sensing	771:777	arg1	range					802:806	a dynamic range	792:806	a dynamic range of 0.01-100 ng/mL	792:824	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	6	51	with	sensing	771:777	arg1	limit					840:844	detection limit	830:844	detection limit of 5.94 pg/mL	830:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	2	52	theme	new	197:199	arg1	platforms					212:220	new analytical platforms	197:220	new analytical platforms	197:220	Design of new analytical platforms is challenging and in demand, addressing molecular capture and subsequent quantitation.
30942566	6	53	theme	direct	748:753	arg1	sensing					771:777	direct electrochemical sensing	748:777	direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL	748:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	1	54	theme	advanced	132:139	arg1	biodiagnostics					141:154	advanced biodiagnostics	132:154	advanced biodiagnostics for research and clinical use	132:184	Detection of biomarkers in biosystems plays a key role in advanced biodiagnostics for research and clinical use.
30942566	7	55	with	reproducibility	988:1002	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	3	56	theme	label-free	333:342	arg1	sensor					360:365	a label-free electrochemical sensor	331:365	a label-free electrochemical sensor for CD44	331:374	Herein, we developed a label-free electrochemical sensor for CD44 by ligand-protein interaction.
30942566	6	57	theme	detection	830:838	arg1	limit					840:844	detection limit	830:844	detection limit of 5.94 pg/mL	830:858	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	7	58	theme	relative	1009:1016	arg1	deviation					1027:1035	relative standard deviation	1009:1035	relative standard deviation (RSD, n = 5) of 2.57%	1009:1057	The sensor also displayed high selectivity, reproducibility with relative standard deviation (RSD, n = 5) of 2.57%, and long-term stability for 14 days.
30942566	3	59	theme	electrochemical	344:358	arg1	sensor					360:365	a label-free electrochemical sensor	331:365	a label-free electrochemical sensor for CD44	331:374	Herein, we developed a label-free electrochemical sensor for CD44 by ligand-protein interaction.
30942566	6	60	theme	current	927:933	arg1	results					935:941	the best current results	918:941	the best current results	918:941	Consequently, we performed direct electrochemical sensing of CD44 with a dynamic range of 0.01-100 ng/mL and detection limit of 5.94 pg/mL without any postlabeling for amplification, comparable to the best current results.
30942566	8	61	theme	human	1156:1160	arg1	serum					1162:1166	human serum	1156:1166	human serum	1156:1166	We demonstrated applications of the sensor in detection of human serum and cancer cells.
30942566	9	62	from	design	1289:1294	arg1	biosystems					1319:1328	given biosystems	1313:1328	given biosystems for diagnosis	1313:1342	Our work guides the development of more sensor types by ligand-protein interactions and contributes to design of interfaces in given biosystems for diagnosis.
30107380	8	0	theme	35S-sulfate	1207:1217	arg1	glycosaminoglycans					1227:1244	35S-sulfate labeled glycosaminoglycans	1207:1244	35S-sulfate labeled glycosaminoglycans	1207:1244	35S-sulfate labeled glycosaminoglycans were characterized by anion exchange and size-exclusion HPLC.
30107380	0	1	theme	Factor	147:152	arg1	Signaling					162:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	0	2	from	Signaling	162:170	arg1	Defective					86:94	Defective	86:94	Defective	86:94	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	11	3	theme	CHO-Positive	1492:1503	arg1	sulfate					1547:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate	1484:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1	1484:1598	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	4	4	theme	surface	608:614	arg1	sulfate					624:630	cell surface heparan sulfate	603:630	cell surface heparan sulfate	603:630	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	11	5	theme	heparan	1539:1545	arg1	sulfate					1547:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate	1484:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1	1484:1598	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	11	6	theme	cell	1526:1529	arg1	sulfate					1547:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate	1484:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1	1484:1598	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	7	7	theme	cell	1146:1149	arg1	glycosaminoglycans					1163:1180	cell association glycosaminoglycans	1146:1180	cell association glycosaminoglycans	1146:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	12	8	contain	had	1711:1713	arg2	length					1764:1769	shorter chain length	1750:1769	shorter chain length	1750:1769	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	8	contain	had	1711:1713	arg1	cells					1705:1709	the CHO-Negative cells	1688:1709	the CHO-Negative cells	1688:1709	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	8	contain	had	1711:1713	arg2	sulfate					1741:1747	heparan sulfate	1733:1747	heparan sulfate	1733:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	8	contain	had	1711:1713	arg2	disaccharides					1794:1806	less 6-O-sulfated disaccharides	1776:1806	less 6-O-sulfated disaccharides	1776:1806	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	8	contain	had	1711:1713	arg2	amount					1723:1728	reduced amount	1715:1728	reduced amount of heparan sulfate	1715:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	1	9	theme	BACKGROUND/AIMS	173:187	arg1	up-regulation					193:205	BACKGROUND/AIMS The up-regulation	173:205	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction	173:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	8	10	theme	anion	1268:1272	arg1	exchange					1274:1281	anion exchange	1268:1281	anion exchange	1268:1281	35S-sulfate labeled glycosaminoglycans were characterized by anion exchange and size-exclusion HPLC.
30107380	16	11	theme	current	2370:2376	arg1	study					2378:2382	current study	2370:2382	current study	2370:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	17	12	theme	HGF/Met	2594:2600	arg1	signaling					2602:2610	HGF/Met signaling	2594:2610	HGF/Met signaling	2594:2610	Such knowledge should be useful in developing glycosaminoglycan-based compounds that target HGF/Met signaling.
30107380	15	13	from	signaling	2265:2273	arg1	cells					2295:2299	the CHO-Negative cells	2278:2299	the CHO-Negative cells	2278:2299	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	4	14	theme	cell	689:692	arg1	sulfate					710:716	specific cell surface heparan sulfate	680:716	specific cell surface heparan sulfate structures	680:727	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	14	15	dep	CONCLUSIONS	1930:1940	arg1	demonstrated					1953:1964	demonstrated	1953:1964	demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.	1953:2141	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	2	16	theme	heparan	321:327	arg1	sulfate					329:335	Specific heparan sulfate	312:335	Specific heparan sulfate structures	312:346	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	5	17	theme	CHO	914:916	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	16	18	theme	unique	2306:2311	arg1	tools					2404:2408	effective tools	2394:2408	effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling	2394:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	16	18	theme	unique	2306:2311	arg1	clones					2348:2353	The unique CHO-Positive and CHO-Negative cell clones	2302:2353	The unique CHO-Positive and CHO-Negative cell clones established in current study	2302:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	7	19	theme	cell	1127:1130	arg1	surface-					1132:1139	cell surface-	1127:1139	cell surface-	1127:1139	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	9	20	theme	sulfate	1316:1322	arg1	compositions					1337:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions were determined by HPLC-MS analysis.
30107380	5	21	theme	Met	861:863	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	6	22	theme	surface	994:1000	arg1	bindings					1002:1009	The cell surface bindings	985:1009	The cell surface bindings	985:1009	The cell surface bindings were imaged by confocal microscopy and flow cytometry analysis.
30107380	5	23	theme	protein-binding	877:891	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	16	24	theme	cell	2343:2346	arg1	tools					2404:2408	effective tools	2394:2408	effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling	2394:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	16	24	theme	cell	2343:2346	arg1	clones					2348:2353	The unique CHO-Positive and CHO-Negative cell clones	2302:2353	The unique CHO-Positive and CHO-Negative cell clones established in current study	2302:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	0	25	theme	Growth	140:145	arg1	Signaling					162:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	5	26	theme	specific	948:955	arg1	sulfate					965:971	specific heparan sulfate	948:971	specific heparan sulfate structures	948:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	12	27	theme	heparan	1733:1739	arg1	sulfate					1741:1747	heparan sulfate	1733:1747	heparan sulfate	1733:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	17	28	theme	Such	2502:2505	arg1	knowledge					2507:2515	Such knowledge	2502:2515	Such knowledge	2502:2515	Such knowledge should be useful in developing glycosaminoglycan-based compounds that target HGF/Met signaling.
30107380	4	29	theme	effective	746:754	arg1	HGF/Met					756:762	effective HGF/Met	746:762	effective HGF/Met	746:762	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	0	30	theme	NK1/Met	154:160	arg1	Signaling					162:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	9	31	theme	HPLC-MS	1369:1375	arg1	analysis					1377:1384	HPLC-MS analysis	1369:1384	HPLC-MS analysis	1369:1384	Heparan sulfate disaccharide compositions were determined by HPLC-MS analysis.
30107380	5	32	theme	sulfate	965:971	arg1	structures					973:982	specific heparan sulfate structures	948:982	specific heparan sulfate structures	948:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	12	33	theme	CHO-Negative	1692:1703	arg1	cells					1705:1709	the CHO-Negative cells	1688:1709	the CHO-Negative cells	1688:1709	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	0	34	from	Defective	86:94	arg1	Binding					117:123	Met Receptor Binding	104:123	Met Receptor Binding	104:123	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	0	34	from	Defective	86:94	arg1	Signaling					162:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	4	35	theme	heparan	702:708	arg1	sulfate					710:716	specific cell surface heparan sulfate	680:716	specific cell surface heparan sulfate structures	680:727	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	16	36	theme	Met	2487:2489	arg1	signaling					2491:2499	Met signaling	2487:2499	Met signaling	2487:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	0	37	theme	Heparan	58:64	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	12	38	theme	CHO-Positive	1828:1839	arg1	cells					1841:1845	CHO-Positive cells	1828:1845	CHO-Positive cells	1828:1845	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	6	39	theme	flow	1050:1053	arg1	analysis					1065:1072	flow cytometry analysis	1050:1072	flow cytometry analysis	1050:1072	The cell surface bindings were imaged by confocal microscopy and flow cytometry analysis.
30107380	0	40	theme	Cell	74:77	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	5	41	from	different	930:938	arg1	structures					973:982	specific heparan sulfate structures	948:982	specific heparan sulfate structures	948:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	14	42	theme	recombinant	1983:1993	arg1	protein					1995:2001	soluble Met recombinant protein	1971:2001	soluble Met recombinant protein	1971:2001	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	13	43	theme	CHO-Negative	1858:1869	arg1	cells					1871:1875	CHO-Negative cells	1858:1875	CHO-Negative cells	1858:1875	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	0	44	theme	Defective	86:94	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	11	45	theme	recombinant	1568:1578	arg1	protein					1580:1586	Met recombinant protein	1564:1586	Met recombinant protein	1564:1586	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	14	46	theme	cell	2012:2015	arg1	HS					2025:2026	cell surface HS	2012:2026	cell surface HS	2012:2026	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	10	47	used	used	1429:1432	arg2	analyses					1400:1407	Western blot analyses	1387:1407	Western blot analyses of MAPK-p42/44	1387:1422	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	10	48	theme	blot	1395:1398	arg1	analyses					1400:1407	Western blot analyses	1387:1407	Western blot analyses of MAPK-p42/44	1387:1422	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	14	49	theme	physiological	2031:2043	arg1	conditions					2045:2054	physiological conditions	2031:2054	physiological conditions	2031:2054	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	0	50	theme	Chinese	36:42	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	15	51	theme	Met	2220:2222	arg1	binding					2224:2230	reduced Met binding	2212:2230	reduced Met binding	2212:2230	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	16	52	theme	structures	2462:2471	arg1	role					2427:2430	the role	2423:2430	the role of specific glycosaminoglycan structures in regulating Met signaling	2423:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	2	53	theme	animal-based	395:406	arg1	systems					414:420	both cell and animal-based model systems	381:420	systems	414:420	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	14	54	theme	Met	1979:1981	arg1	protein					1995:2001	soluble Met recombinant protein	1971:2001	soluble Met recombinant protein	1971:2001	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	15	55	theme	reduced	2165:2171	arg1	6-O-sulfation					2173:2185	reduced 6-O-sulfation	2165:2185	reduced 6-O-sulfation	2165:2185	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	15	56	theme	CHO-Negative	2282:2293	arg1	cells					2295:2299	the CHO-Negative cells	2278:2299	the CHO-Negative cells	2278:2299	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	1	57	theme	human	297:301	arg1	cancers					303:309	human cancers	297:309	human cancers	297:309	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	12	58	theme	Overall	1630:1636	arg1	results					1665:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	59	theme	6-O-sulfated	1781:1792	arg1	disaccharides					1794:1806	less 6-O-sulfated disaccharides	1776:1806	less 6-O-sulfated disaccharides	1776:1806	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	15	60	theme	diminished	2240:2249	arg1	signaling					2265:2273	the diminished NK1-initiated signaling	2236:2273	the diminished NK1-initiated signaling	2236:2273	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	14	61	theme	surface	2017:2023	arg1	HS					2025:2026	cell surface HS	2012:2026	cell surface HS	2012:2026	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	16	62	theme	glycosaminoglycan	2444:2460	arg1	structures					2462:2471	specific glycosaminoglycan structures	2435:2471	specific glycosaminoglycan structures	2435:2471	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	5	63	theme	sorting	814:820	arg1	strategy					822:829	An established flow sorting strategy	794:829	An established flow sorting strategy	794:829	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	12	64	theme	analysis	1656:1663	arg1	results					1665:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	65	theme	reduced	1715:1721	arg1	amount					1723:1728	reduced amount	1715:1728	reduced amount of heparan sulfate	1715:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	65	theme	reduced	1715:1721	arg1	sulfate					1741:1747	heparan sulfate	1733:1747	heparan sulfate	1733:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	16	66	theme	effective	2394:2402	arg1	clones					2348:2353	The unique CHO-Positive and CHO-Negative cell clones	2302:2353	The unique CHO-Positive and CHO-Negative cell clones established in current study	2302:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	16	66	theme	effective	2394:2402	arg1	tools					2404:2408	effective tools	2394:2408	effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling	2394:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	7	67	from	glycosaminoglycans	1163:1180	arg1	contents					1105:1112	Glucosamine vs. galactosamine contents	1075:1112	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans	1075:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	15	68	theme	Shorter	2143:2149	arg1	chains					2154:2159	Shorter HS chains	2143:2159	Shorter HS chains	2143:2159	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	10	69	theme	HGF-	1445:1448	arg1	signaling					1473:1481	HGF- and NK1-facillated Met signaling	1445:1481	HGF- and NK1-facillated Met signaling	1445:1481	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	3	70	theme	splicing	516:523	arg1	NK1					533:535	a natural occurring splicing variant NK1	496:535	a natural occurring splicing variant NK1 of HGF	496:542	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	0	71	theme	Hamster	44:50	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	17	72	theme	glycosaminoglycan-based	2548:2570	arg1	compounds					2572:2580	glycosaminoglycan-based compounds	2548:2580	glycosaminoglycan-based compounds that target HGF/Met signaling	2548:2610	Such knowledge should be useful in developing glycosaminoglycan-based compounds that target HGF/Met signaling.
30107380	3	73	theme	natural	498:504	arg1	NK1					533:535	a natural occurring splicing variant NK1	496:535	a natural occurring splicing variant NK1 of HGF	496:542	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	5	74	used	used	835:838	arg2	strategy					822:829	An established flow sorting strategy	794:829	An established flow sorting strategy	794:829	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	4	75	theme	heparan	616:622	arg1	sulfate					624:630	cell surface heparan sulfate	603:630	cell surface heparan sulfate	603:630	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	3	76	theme	HGF	540:542	arg1	HGF					488:490	HGF	488:490	HGF	488:490	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	3	76	theme	HGF	540:542	arg1	NK1					533:535	a natural occurring splicing variant NK1	496:535	a natural occurring splicing variant NK1 of HGF	496:542	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	1	77	located	observed	277:284	arg1	most					289:292	most	289:292	most	289:292	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	77	located	observed	277:284	arg1	cancers					303:309	human cancers	297:309	human cancers	297:309	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	77	located	observed	277:284	arg2	up-regulation					193:205	BACKGROUND/AIMS The up-regulation	173:205	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction	173:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	10	78	theme	NK1-facillated	1454:1467	arg1	signaling					1473:1481	HGF- and NK1-facillated Met signaling	1445:1481	HGF- and NK1-facillated Met signaling	1445:1481	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	15	79	from	binding	2224:2230	arg1	cells					2295:2299	the CHO-Negative cells	2278:2299	the CHO-Negative cells	2278:2299	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	7	80	from	media-	1119:1124	arg1	contents					1105:1112	Glucosamine vs. galactosamine contents	1075:1112	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans	1075:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	11	81	theme	surface	1531:1537	arg1	sulfate					1547:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate	1484:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1	1484:1598	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	7	82	theme	association	1151:1161	arg1	glycosaminoglycans					1163:1180	cell association glycosaminoglycans	1146:1180	cell association glycosaminoglycans	1146:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	8	83	theme	labeled	1219:1225	arg1	glycosaminoglycans					1227:1244	35S-sulfate labeled glycosaminoglycans	1207:1244	35S-sulfate labeled glycosaminoglycans	1207:1244	35S-sulfate labeled glycosaminoglycans were characterized by anion exchange and size-exclusion HPLC.
30107380	4	84	theme	cell	603:606	arg1	sulfate					624:630	cell surface heparan sulfate	603:630	cell surface heparan sulfate	603:630	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	11	85	theme	CHO-Negative	1513:1524	arg1	sulfate					1547:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate	1484:1553	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1	1484:1598	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	2	86	theme	HGF/Met	360:366	arg1	signaling					368:376	the HGF/Met signaling	356:376	the HGF/Met signaling	356:376	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	3	87	theme	similar	549:555	arg1	affinity					557:564	similar affinity	549:564	similar affinity	549:564	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	5	88	theme	positive	893:900	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	5	89	theme	established	797:807	arg1	strategy					822:829	An established flow sorting strategy	794:829	An established flow sorting strategy	794:829	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	2	90	theme	sulfate	329:335	arg1	structures					337:346	Specific heparan sulfate structures	312:346	Specific heparan sulfate structures	312:346	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	5	91	theme	negative	905:912	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	12	92	theme	shorter	1750:1756	arg1	length					1764:1769	shorter chain length	1750:1769	shorter chain length	1750:1769	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	4	93	theme	specific	680:687	arg1	sulfate					710:716	specific cell surface heparan sulfate	680:716	specific cell surface heparan sulfate structures	680:727	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	13	94	theme	HGF/Met	1911:1917	arg1	signaling					1919:1927	HGF/Met signaling	1911:1927	NK1/Met but not HGF/Met signaling	1895:1927	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	9	95	theme	Heparan	1308:1314	arg1	compositions					1337:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions were determined by HPLC-MS analysis.
30107380	2	96	theme	Specific	312:319	arg1	sulfate					329:335	Specific heparan sulfate	312:335	Specific heparan sulfate structures	312:346	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	5	97	theme	soluble	853:859	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	11	98	dep	CHO-Positive	1492:1503	arg1	RESULTS					1484:1490	RESULTS	1484:1490	RESULTS	1484:1490	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	16	99	theme	CHO-Negative	2330:2341	arg1	tools					2404:2408	effective tools	2394:2408	effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling	2394:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	16	99	theme	CHO-Negative	2330:2341	arg1	clones					2348:2353	The unique CHO-Positive and CHO-Negative cell clones	2302:2353	The unique CHO-Positive and CHO-Negative cell clones established in current study	2302:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	0	100	theme	Hepatocyte	129:138	arg1	Signaling					162:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Hepatocyte Growth Factor NK1/Met Signaling	129:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	9	101	theme	disaccharide	1324:1335	arg1	compositions					1337:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions	1308:1348	Heparan sulfate disaccharide compositions were determined by HPLC-MS analysis.
30107380	14	102	theme	Met	2062:2064	arg1	/HGF					2066:2069	a Met /HGF	2060:2069	a Met /HGF	2060:2069	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	5	103	theme	recombinant	865:875	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	8	104	theme	size-exclusion	1287:1300	arg1	HPLC					1302:1305	size-exclusion HPLC	1287:1305	size-exclusion HPLC	1287:1305	35S-sulfate labeled glycosaminoglycans were characterized by anion exchange and size-exclusion HPLC.
30107380	6	105	theme	cell	989:992	arg1	bindings					1002:1009	The cell surface bindings	985:1009	The cell surface bindings	985:1009	The cell surface bindings were imaged by confocal microscopy and flow cytometry analysis.
30107380	5	106	dep	METHODS	786:792	arg1	used					835:838	used	835:838	was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	831:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	16	107	theme	CHO-Positive	2313:2324	arg1	tools					2404:2408	effective tools	2394:2408	effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling	2394:2499	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	16	107	theme	CHO-Positive	2313:2324	arg1	clones					2348:2353	The unique CHO-Positive and CHO-Negative cell clones	2302:2353	The unique CHO-Positive and CHO-Negative cell clones established in current study	2302:2382	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	4	108	theme	physiological	648:660	arg1	conditions					662:671	physiological conditions	648:671	physiological conditions	648:671	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	7	109	theme	galactosamine	1091:1103	arg1	contents					1105:1112	Glucosamine vs. galactosamine contents	1075:1112	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans	1075:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	12	110	theme	sulfate	1741:1747	arg1	amount					1723:1728	reduced amount	1715:1728	reduced amount of heparan sulfate	1715:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	110	theme	sulfate	1741:1747	arg1	sulfate					1741:1747	heparan sulfate	1733:1747	heparan sulfate	1733:1747	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	110	theme	sulfate	1741:1747	arg1	disaccharides					1794:1806	less 6-O-sulfated disaccharides	1776:1806	less 6-O-sulfated disaccharides	1776:1806	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	12	110	theme	sulfate	1741:1747	arg1	length					1764:1769	shorter chain length	1750:1769	shorter chain length	1750:1769	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	7	111	theme	Glucosamine	1075:1085	arg1	contents					1105:1112	Glucosamine vs. galactosamine contents	1075:1112	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans	1075:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	5	112	theme	heparan	957:963	arg1	sulfate					965:971	specific heparan sulfate	948:971	specific heparan sulfate structures	948:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	0	113	theme	Sulfate	66:72	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	5	114	theme	cell	918:921	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	6	115	theme	cytometry	1055:1063	arg1	analysis					1065:1072	flow cytometry analysis	1050:1072	flow cytometry analysis	1050:1072	The cell surface bindings were imaged by confocal microscopy and flow cytometry analysis.
30107380	4	116	theme	surface	694:700	arg1	sulfate					710:716	specific cell surface heparan sulfate	680:716	specific cell surface heparan sulfate structures	680:727	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	0	117	theme	Mutant	79:84	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	0	117	theme	Mutant	79:84	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	13	118	from	NK1/Met	1895:1901	arg1	defective					1882:1890	defective	1882:1890	defective	1882:1890	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	5	119	theme	different	930:938	arg1	clones					923:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones	851:928	a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures	851:982	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	0	120	theme	Receptor	108:115	arg1	Binding					117:123	Met Receptor Binding	104:123	Met Receptor Binding	104:123	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	4	121	theme	sulfate	710:716	arg1	structures					718:727	specific cell surface heparan sulfate structures	680:727	specific cell surface heparan sulfate structures	680:727	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	13	122	from	defective	1882:1890	arg1	NK1/Met					1895:1901	NK1/Met	1895:1901	NK1/Met but not HGF/Met signaling	1895:1927	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	13	122	from	defective	1882:1890	arg1	signaling					1919:1927	HGF/Met signaling	1911:1927	NK1/Met but not HGF/Met signaling	1895:1927	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	6	123	theme	confocal	1026:1033	arg1	microscopy					1035:1044	confocal microscopy	1026:1044	confocal microscopy	1026:1044	The cell surface bindings were imaged by confocal microscopy and flow cytometry analysis.
30107380	0	124	theme	Met	104:106	arg1	Binding					117:123	Met Receptor Binding	104:123	Met Receptor Binding	104:123	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	13	125	from	signaling	1919:1927	arg1	defective					1882:1890	defective	1882:1890	defective	1882:1890	Moreover, CHO-Negative cells were defective in NK1/Met but not HGF/Met signaling.
30107380	1	126	theme	hepatocyte	210:219	arg1	HGF/Met					245:251	HGF/Met	245:251	HGF/Met	245:251	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	126	theme	hepatocyte	210:219	arg1	transduction					261:272	signal transduction	254:272	signal transduction	254:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	126	theme	hepatocyte	210:219	arg1	factor/receptor					228:242	hepatocyte growth factor/receptor	210:242	hepatocyte growth factor/receptor	210:242	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	15	127	theme	reduced	2212:2218	arg1	binding					2224:2230	reduced Met binding	2212:2230	reduced Met binding	2212:2230	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	10	128	theme	Western	1387:1393	arg1	analyses					1400:1407	Western blot analyses	1387:1407	Western blot analyses of MAPK-p42/44	1387:1422	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	2	129	theme	model	408:412	arg1	systems					414:420	both cell and animal-based model systems	381:420	systems	414:420	Specific heparan sulfate structures enhance the HGF/Met signaling at both cell and animal-based model systems.
30107380	12	130	theme	chain	1758:1762	arg1	length					1764:1769	shorter chain length	1750:1769	shorter chain length	1750:1769	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	7	131	from	surface-	1132:1139	arg1	contents					1105:1112	Glucosamine vs. galactosamine contents	1075:1112	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans	1075:1180	Glucosamine vs. galactosamine contents from media-, cell surface-, and cell association glycosaminoglycans were quantified by HPLC.
30107380	16	132	theme	specific	2435:2442	arg1	structures					2462:2471	specific glycosaminoglycan structures	2435:2471	specific glycosaminoglycan structures	2435:2471	The unique CHO-Positive and CHO-Negative cell clones established in current study should be effective tools for studying the role of specific glycosaminoglycan structures in regulating Met signaling.
30107380	3	133	theme	heparan	457:463	arg1	sulfate					465:471	heparan sulfate	457:471	heparan sulfate	457:471	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	14	134	theme	soluble	1971:1977	arg1	protein					1995:2001	soluble Met recombinant protein	1971:2001	soluble Met recombinant protein	1971:2001	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	4	135	theme	NK1/Met	767:773	arg1	signaling					775:783	NK1/Met signaling	767:783	NK1/Met signaling	767:783	However, it is currently unknown if cell surface heparan sulfate binds to Met at physiological conditions and if specific cell surface heparan sulfate structures are required for effective HGF/Met or NK1/Met signaling.
30107380	10	136	theme	MAPK-p42/44	1412:1422	arg1	analyses					1400:1407	Western blot analyses	1387:1407	Western blot analyses of MAPK-p42/44	1387:1422	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	5	137	theme	flow	809:812	arg1	strategy					822:829	An established flow sorting strategy	794:829	An established flow sorting strategy	794:829	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	11	138	theme	Met	1564:1566	arg1	protein					1580:1586	Met recombinant protein	1564:1586	Met recombinant protein	1564:1586	RESULTS CHO-Positive but not CHO-Negative cell surface heparan sulfate bound to Met recombinant protein and HGF/NK1 further promoted the binding.
30107380	1	139	theme	growth	221:226	arg1	HGF/Met					245:251	HGF/Met	245:251	HGF/Met	245:251	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	139	theme	growth	221:226	arg1	transduction					261:272	signal transduction	254:272	signal transduction	254:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	139	theme	growth	221:226	arg1	factor/receptor					228:242	hepatocyte growth factor/receptor	210:242	hepatocyte growth factor/receptor	210:242	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	14	140	theme	Met	2128:2130	arg1	signaling					2132:2140	Met signaling	2128:2140	Met signaling	2128:2140	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	0	141	theme	Ovary	52:56	arg1	Mutant					79:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant	34:84	a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling	34:170	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	12	142	theme	glycosaminoglycan	1638:1654	arg1	results					1665:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results	1630:1671	Overall glycosaminoglycan analysis results indicated that the CHO-Negative cells had reduced amount of heparan sulfate, shorter chain length, and less 6-O-sulfated disaccharides compared to that of CHO-Positive cells.
30107380	3	143	theme	Biochemical	423:433	arg1	studies					435:441	Biochemical studies	423:441	Biochemical studies	423:441	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	15	144	theme	HS	2151:2152	arg1	chains					2154:2159	Shorter HS chains	2143:2159	Shorter HS chains	2143:2159	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	14	145	theme	NK1/HS	2074:2079	arg1	complex					2099:2105	NK1/HS ternary signaling complex	2074:2105	NK1/HS ternary signaling complex	2074:2105	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	3	146	theme	variant	525:531	arg1	NK1					533:535	a natural occurring splicing variant NK1	496:535	a natural occurring splicing variant NK1 of HGF	496:542	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	14	147	theme	ternary	2081:2087	arg1	complex					2099:2105	NK1/HS ternary signaling complex	2074:2105	NK1/HS ternary signaling complex	2074:2105	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	15	148	theme	NK1-initiated	2251:2263	arg1	signaling					2265:2273	the diminished NK1-initiated signaling	2236:2273	the diminished NK1-initiated signaling	2236:2273	Shorter HS chains and reduced 6-O-sulfation might be responsible for reduced Met binding and the diminished NK1-initiated signaling in the CHO-Negative cells.
30107380	3	149	theme	occurring	506:514	arg1	NK1					533:535	a natural occurring splicing variant NK1	496:535	a natural occurring splicing variant NK1 of HGF	496:542	Biochemical studies indicate that heparan sulfate interacts with HGF and a natural occurring splicing variant NK1 of HGF with similar affinity.
30107380	5	150	from	structures	973:982	arg1	different					930:938	different	930:938	different	930:938	METHODS An established flow sorting strategy was used to isolate a soluble Met recombinant protein-binding positive or negative CHO cell clones different only in specific heparan sulfate structures.
30107380	1	151	theme	factor/receptor	228:242	arg1	up-regulation					193:205	BACKGROUND/AIMS The up-regulation	173:205	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction	173:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	14	152	theme	signaling	2089:2097	arg1	complex					2099:2105	NK1/HS ternary signaling complex	2074:2105	NK1/HS ternary signaling complex	2074:2105	CONCLUSIONS This study demonstrated that soluble Met recombinant protein bound to cell surface HS at physiological conditions and a Met /HGF or NK1/HS ternary signaling complex might be involved in Met signaling.
30107380	0	153	from	Binding	117:123	arg1	Defective					86:94	Defective	86:94	Defective	86:94	Isolation and Characterization of a Chinese Hamster Ovary Heparan Sulfate Cell Mutant Defective in Both Met Receptor Binding and Hepatocyte Growth Factor NK1/Met Signaling.
30107380	10	154	theme	Met	1469:1471	arg1	signaling					1473:1481	HGF- and NK1-facillated Met signaling	1445:1481	HGF- and NK1-facillated Met signaling	1445:1481	Western blot analyses of MAPK-p42/44 were used to monitor HGF- and NK1-facillated Met signaling.
30107380	1	155	theme	signal	254:259	arg1	transduction					261:272	signal transduction	254:272	signal transduction	254:272	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.
30107380	1	155	theme	signal	254:259	arg1	factor/receptor					228:242	hepatocyte growth factor/receptor	210:242	hepatocyte growth factor/receptor	210:242	BACKGROUND/AIMS The up-regulation of hepatocyte growth factor/receptor, HGF/Met, signal transduction is observed in most of human cancers.